LOCUS       DASRZF010000001         2967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152 k141_1704,
            whole genome shotgun sequence.
ACCESSION   DASRZF010000001 DASRZF010000000
VERSION     DASRZF010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1704"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..2775)
                     /locus_tag="VFG22_00005"
     CDS             complement(115..2775)
                     /locus_tag="VFG22_00005"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HET6414643.1"
                     /translation="MNVVNLRSREARSRTLCGGKGAGLAALIDAAMPVPEGFVVTTAA
                     FTEFLAHNGLESACSELDHVELQRRVELGERLRQGISTGDFPRSIAEGIQGMLAAIEE
                     RIGQRPLWAVRSSAIAEDTDRASFAGQYDTSLGVAASQITEAVRHSWRSSFSEHAIRY
                     RAQQRTDENRMAVLVQVLVHAETAGVCFTIDPLTGEPRIVINANYGFGESVVGGQVTP
                     DTFILDRLDTKSILEIAGHKHTQTIVGANGVEAIEVSADKQDSLCLSPAEATEIARLA
                     LSVEKLHGGPVDVEWAYQGAQLFLLQARSITSERASSSPPPKDWIPASNTQIDPKYPL
                     YSNGNISEVLPGCVTPLSWDHTGQLIEHAFQTQLEALGAIDPSDEAPSVLGFFYHRPY
                     INVSLLLEAATRTPGMTPDTVHEELIGKPESRTPALRAADFLPHRFGRLWRVLRVVLV
                     HARTLSSRIEACRHSAESDETRVSDAWLTSASDELLLEQVRMNADLAAPSVVHVWAST
                     LASVAFTQLRRFTARWLGDEDGALASELVTGIEHLPSIAPALALQGLCEKIARSEDLA
                     MLFRSDADDTAILEALRSDPLNQELEDFLSRYGHRGVSEAELQRSCWREDPAQVVSLM
                     RNNFRPGAATPIDVFARQRRAHEAAMGKLDVLSAWRRFWLRALIRRARSGLLNRETMK
                     DLVIRRLDRSRRVYRELNRRLVARHLIVSPDDMFFLRWSELRDLVTGTWSVREAAQLM
                     GERQRDYRWSRQVDVPKLQRGTPRVLDPEELKYDLELAGMGVSPGRITGTARVVTDPR
                     HGAYIEPGEILVAPVTDVAWTPLFAQAGGLVVEVGGLLSHGSIVAREYGVPTVVGVAG
                     ATRSIRTGDRISVDGSSGRVRKLEAVEIRR"
     gene            complement(2772..>2967)
                     /locus_tag="VFG22_00010"
     CDS             complement(2772..>2967)
                     /locus_tag="VFG22_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414644.1"
                     /translation="PDAVGLVLTAKSGYQFALEAEVVTPLPVIAKTEREDVLVTAARA
                     KYFWGERVAQGMVEFMEQLS"
BASE COUNT          549 a          944 c          928 g          546 t
ORIGIN      
        1 ggcaagcatt tgcgtacttc gcatcctgga cggtgggcat cggcaccatc ttgcagaggc
       61 tgcagcgggt gcacttctgc atctccgttc gcaaggactc gaggcgccgc ttcgtcaccg
      121 tcgaatctcc acggcctcga gtttgcgcac gcgtccgcta gagccgtcca cgctgatcct
      181 atcgcccgtc cgaatcgagc gcgtcgctcc agcgacgcca accacggtcg gaaccccata
      241 ttcgcgcgcg actatcgaac cgtgcgagag caacccgccg acttcgacga ccagcccacc
      301 ggcctgcgcg aaaaggggag tccaggctac gtcggtgacg ggcgccacca gaatctcacc
      361 aggctcgatg taggcaccgt gccgtgggtc ggtgaccacg cgggcggttc ctgtgattcg
      421 acccgggctc acccccatcc cggcgagctc gaggtcgtac ttcagctcct cgggatcgag
      481 aacgcgaggc gtcccacgct gtagcttcgg gacatcgact tgccgcgacc atcggtagtc
      541 gcgttgccgt tctcccatga gctgggcagc ctctctcaca ctccaagtcc cggtcacgag
      601 atctcgcagc tcagaccagc gcaagaaaaa catatcatcg ggagagacga tcaagtgacg
      661 cgcgacgagt cggcggttga gctcgcggta cacccgtctc gagcgatcga gtctacgaat
      721 taccaagtcc ttcatcgttt cacggttcaa caaacccgac cgggcccttc gaatcagggc
      781 tctcaaccag aagcggcgcc atgcgctcag gacgtcgagc tttcccatcg cggcttcgtg
      841 cgcgcgccgc tgccgcgcga agacatcgat cggcgtggcc gcaccggggc ggaagttgtt
      901 gcgcatgaga gagacgactt gcgctgggtc ttcacgccag cacgacctct ggagctcggc
      961 ttctgaaacg cctcgatggc cgtaccggga aaggaagtcc tcgagctcct ggttcaaagg
     1021 gtcagaccga agcgcctcga ggattgccgt gtcgtccgcg tcacttcgaa aaagcatcgc
     1081 gagatcttcg gagcgagcga tcttttcgca gagtccttgc agggcgagag ctggtgcgat
     1141 gctcggcaga tgctcgattc cagtgacgag ctcggacgcc aatgccccat cctcatcgcc
     1201 gagccatcgc gcagtgaatc gacggagctg agtgaaggca accgacgcca gcgtcgatgc
     1261 ccagacatga acgaccgagg gggcagccag atcggcgttc attcgaacct gctccaggag
     1321 caattcgtca gacgcgctcg tcaaccaggc gtccgagacc cgggtctcat cggattctgc
     1381 gctgtggcgg caggcttcga ttcgactcga aagcgtgcgc gcgtgaacca gcacgacacg
     1441 gagcacccgc caaagacggc cgaagcggtg cggcaagaaa tccgccgcgc gcaaagccgg
     1501 cgtccggctc tccggtttgc cgatcaattc ctcgtgaact gtgtcgggcg tcatgcccgg
     1561 cgtacgcgtg gcggcctcca ggagcagcga gacgttgatg tagggtcgat gatagaagaa
     1621 cccgagcacg ctcggcgcct cgtccgaggg atcgatcgcg cccagcgctt cgagttgtgt
     1681 ttggaacgcg tgctcgatca gttggcccgt gtgatcccag gacaagggtg tgacgcagcc
     1741 cgggaggact tcgctgatgt ttccgttcga gtagagcgga tacttcgggt cgatttgggt
     1801 gttgctggcg ggaatccagt ccttgggtgg ggggctagag ctcgctcgct cggaagtgat
     1861 cgagcgtgcc tgaagcagga agagttgagc gccttggtac gcccattcga cgtcgaccgg
     1921 gccgccgtgc agcttctcca cggagagcgc aagccgtgcg atctcggttg cctcagcggg
     1981 actgaggcac agcgagtctt gcttgtcggc ggagacctcg atggcctcaa cgccgttggc
     2041 tcccacgatc gtttgggtgt gcttgtgacc cgcaatttcc agaatggact tcgtgtcgag
     2101 cctgtccaag atgaacgtat ccggggtcac ctgacctccg acgacggact caccgaatcc
     2161 gtagttcgcg ttgatgacga tgcgaggctc tccggtcagc ggatcgatgg tgaagcacac
     2221 gcctgcggtt tcggcgtgaa caaggacctg gacgagcacc gccatgcgat tttcgtcggt
     2281 tcgctgctgc gcacggtagc gaatggcgtg ctcgctgaac gatgagcgcc aggaatggcg
     2341 cacggcctcc gtgatctggc tcgcggccac gccaagactc gtgtcgtact gacccgcgaa
     2401 gctcgcgcgg tccgtgtctt ccgcgatcgc cgacgagcgc accgcccaga gtgggcgttg
     2461 cccgatgcgc tcctcgattg ccgcaagcat cccttgaatc ccttcggcga tcgagcgagg
     2521 gaagtcgccg gtcgagattc cctgccgaag ccgttccccg agctccacgc gtcgctggag
     2581 ctccacatga tcaagctcgg agcacgcgga ttcgaggcca ttgtgcgcta gaaactcggt
     2641 gaatgcggcc gtcgtgacga cgaatccttc cggaaccggc attgcggcat cgatcagcgc
     2701 ggcgagtccc gctcccttgc caccgcaaag cgtccgcgat ctggcctccc gcgagcgaag
     2761 gttcacgaca ttcacgagag ctgctccatg aactccacca ttccctgggc gacccgttct
     2821 ccccagaaat atttcgcgcg ggcggcggtc accagaacgt cttcgcgttc ggtctttgcg
     2881 atgaccggca gtggtgtgac gacctcagct tcgagagcaa actggtaccc agactttgcg
     2941 gtgagcacga gccccacggc gtcgggc
//
LOCUS       DASRZF010000002        26272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3441737, whole genome shotgun sequence.
ACCESSION   DASRZF010000002 DASRZF010000000
VERSION     DASRZF010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 26272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26272
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3441737"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VFG22_00015"
     CDS             complement(<1..328)
                     /locus_tag="VFG22_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HET6414645.1"
                     /translation="MSRNRLGELLVREKRISLEQLREAQKQQKDDNVSLGYALARLGA
                     ISDQEITDFLSEQYRVQSIDLSEYEIDREILKLISPQVCERHKIIPVSRAGSSLIVAM
                     ADPSNLN"
     gene            579..1475
                     /locus_tag="VFG22_00020"
     CDS             579..1475
                     /locus_tag="VFG22_00020"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6414646.1"
                     /translation="MAKPKKQITAAPKDTAEALEQIEQPKMNWKVLGLIGLALAVLWV
                     IAGILQPTIGYVGLIVAGVLTLVALGFLIWVWRLTRKSADIAAILKGATDKEGRLAAL
                     EQLRARQLPGGKDALNAIAQAQLVAQEDPAEAIRILEGIEINKAPAMIQDDVRANLAM
                     FYLMTGRARDARELANEIRLDRQPQAKAKAMYASVVAESYARTGDAEEARKLLETYDP
                     KDKEFGQVSGMLLRAQVYTFSATKKRGRAREAMEQLLEVDPNMVAAFVQKGTRPELSR
                     LAKQVLANAGALPKPKMRVRTR"
     gene            1482..2339
                     /gene="prmC"
                     /locus_tag="VFG22_00025"
     CDS             1482..2339
                     /gene="prmC"
                     /locus_tag="VFG22_00025"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HET6414647.1"
                     /translation="MTSRTWTIREVLEWTAKDFASRGITSARLDAELLVAKALEMDRV
                     GLYLDLNRPLDEIERGEIRLLVARRREREPVAYILGHRDFYGRRFVVTADVLIPRPDT
                     ETLAERALECIPKVLGCRVLDVGTGSGALAITIAAERPLADVTATDISTAALEVASKN
                     ARRLEVADRIQFQCTDLLESGHQYDVIVSNPPYITRRDMETLPADVRQYEPRLALEAG
                     EDGLDVVRALLAACEPAAQQGSQLLIEIGAGQAASVLDIAKSRSSWELVAVYPDLNRI
                     ERVVHLRRI"
     gene            complement(2321..4276)
                     /locus_tag="VFG22_00030"
     CDS             complement(2321..4276)
                     /locus_tag="VFG22_00030"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HET6414648.1"
                     /translation="MGFGVPWALAGLIAVGIPILAHLLRRQKLPRQTLPTIAFLHRIH
                     ATRAARLRVVDMLLLIVRTLLVALLALVAAAPYRVVHSSLLDRQHALAIVLDDSLSMA
                     AIHEGETLIEQAAALAAEQVRSLPEGSEVSVVLSGKPARVWLRKRSELEMVETVFGEI
                     DTRSTRGNALCDAVQAAVELLLESELPDRRLLVLSDFTPGARADECEWPTRGIRVGFE
                     TVSTPNPENRRIGSVDVLGENDAAEGLRLEVEIVGPPSAVQQRVRLRQKGQETQKATV
                     ERTEAGGEAVLFIKAGQTDAVVELQLDPDDVLPADNVRGVLLGSGTVHVLLVDGDPHP
                     DPRKRETAFLNHALSVAPRVGRSFAVETLDAQDLSESAIIRSDVLVLANATLDEKWLP
                     LIQRFVSGGGSLWITAGDRINPRAYGSVLRELLPGRYTATRMEESPLSLESESLPELD
                     QAKVMGRTPIAEASLDAVVTAQFSDGSPAMLEHRYRSGRVAMFATTVDDAWTDLPYQP
                     VFVPFVLDTLARLAGRSDHSVEPYAPVSLPGSTSTQRIQVVTPSGRTLDLAEADRVAF
                     RDTGEIGAYRVLVNGVNGARFAFVVDAPVGESDLVSKGPPDAESSIEAVDSEASAGVE
                     QPLAPWLLLLAALLVFTELWLRQIRRR"
     gene            complement(4280..5155)
                     /locus_tag="VFG22_00035"
     CDS             complement(4280..5155)
                     /locus_tag="VFG22_00035"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET6414649.1"
                     /translation="MTHVLEPELAVRLAGLTLRAKRTVDGLLAGMHRSPHRGASAIFK
                     EHRAYRPGDDPRLIDWRAYARSDRYSIKHFEHETQLRGTLALDISRSMRWGSGDVSKL
                     DHAATLLAALAYVLTRQGDAVSLITFDDAIRDRLPARSGPAHLDYLMQTLAAPSPSTG
                     ATALHASIDKLIEHAGRMGLVVIASDLLDLEQDALDPVARIQAKGHQTVVLHVMDPAE
                     LELPYEQPMHFEGLEGELPVEVDPKGIRRRYKESIDAFVASCQHRVVAAGGRYLLART
                     DTPPEHTLLQLLRDG"
     gene            complement(5152..6183)
                     /locus_tag="VFG22_00040"
     CDS             complement(5152..6183)
                     /locus_tag="VFG22_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET6414650.1"
                     /translation="MSEPVRSSPPAAPNGATSDVLRVQQLSDARDEIISRLKQRIVGQ
                     EDVIELLLIALFARGHGLFVGVPGLAKTLLITTLADALSLHSNRIQFTPDLMPSDVTG
                     TDVLEEDATSGKRHFRFIPGPVFTNILLADEINRAPPKTQAALLQSMAEGQVTASGNT
                     YALEQPYLVFATQNPIEQEGTYPLPEAQLDRFLFQIDVRYPSQHEEIDIVKRTTAPMP
                     TSITPVLSAQDIVGFQDLVPRVPAADHVIQHAVTLVRASRPEDPRASELVRKNVAFGA
                     GPRASQGLILGAKARAILHGRYAAEIQDVRALVEPILTHRLVMNFRAEAEGVTSADVI
                     ESIKQSIEP"
     gene            complement(6221..7774)
                     /locus_tag="VFG22_00045"
     CDS             complement(6221..7774)
                     /locus_tag="VFG22_00045"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6414651.1"
                     /translation="MNRLPLFACCLLLGSLATSAPAENATSSEVLDLLEASLAAGEGV
                     PAQILVRKYVEQFPRTSRVMELEVLAEFYSGDYEAAANGIAELRKDPRYGTELGDLVD
                     VIVDTYETTKNYETVVYENFEVRYAPGDDEILVPYAIDALKAATEALEAELGVKMVSP
                     VRLEIYPDAESLARVSTLSVDAIRNTGTIALCKWGRLMIASPGALMHGYPWLDTIVHE
                     YVHLLLTKASLDRAPVWLQEGLAKFLETRWRQDEASLRHDPAATQLLHEAARADQLLD
                     FDQMHPSIALLPTQKVAALAFAQVSSFMAMYYREFGRDGVQKALERVGAGEDARKALA
                     RVAGVSWKTLETRWRSSLSSDPDSPPARLMPLYLSGEATEQDEIAGVELDRARNYVRL
                     GDLLWTRSRPAAASVEYAKAHEAAPADPVVASRYARSAIAGGRPQNAIAPLKRTLERY
                     PTHAPALSSLATALFRMSNFGGARDAANEAIAHNPFDPQPHCILSKLPLPQAEHEATL
                     CSRLGGMRE"
     gene            complement(7771..10698)
                     /locus_tag="VFG22_00050"
     CDS             complement(7771..10698)
                     /locus_tag="VFG22_00050"
                     /inference="COORDINATES: protein motif:HMM:NF025152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HET6414652.1"
                     /translation="MSSTTGRLAEYRRNLRLAAATVMALRISAAAAAVGLASFVIVAW
                     ALGPMTPVVWLCIGWSVVFGMTTGAVAWSIQPLVKLRGHGALGLVATGQPSLLSPLQS
                     AYELQDEQAFSRELVIAQRLGLLRALDASPARKFIPWRWLVQPALAAALILAAAAWWS
                     LDFDRPTAGRYALLHADLGEVEGVRTSHVVAHTGADLFFPEYMQRVNERIEEADRLIV
                     PRGTAIEYAITPIGDAARVVVELPGRHVEAKRAGEQFVASFVADTDGPLEIYVESTRG
                     ERRVDHRARSISVQVDQPPRVTLIAPEDDLVVEERQPVLLEHSATDDYGVQELTLIIK
                     LPNGEHLRRAILAPSQETKNDFYGETALRLEEFSLSPADAVTIWLEAKDANRVDGPGI
                     GRSAVRTLTLASEITRRRDRIVELEQLLNTVLSALAVRLEQPLPKRLDEARRRRDASM
                     VPYRGVMSLLANVGDHRGPRTVPLLLDMRKRLERAGRREDSAYLSAAQVSNRHRFDKA
                     IVSELEKDTLIVADLISEARLNDAAEIAREMQQLRQEIASLIGELRRGQTPELQRALM
                     AALDRAERRMGALRDQLRQAMQYVPGEFVNRQAQQANKSTDALRSVREALAAGDLDAA
                     EAALTKLDQTIDAMTQALAQSEDSLVEARFGPRERALMKATDTIRDLEVEQKRMADKA
                     RRAGNKAVDRVSGDKKSMASEIRGELEKIAKEVSELLGGLNDEALGPYESSLQGRASE
                     RLSDFQKALEAADLGEALSMANRLAQAADQLAKDLELSAMMFRGRGGQTAEAAAQARQ
                     VAARAQDLRDAAQGALPDIANALTMGERQELAKQAAQQGDAKEATHQLARRLREEVGG
                     VPVSEEAAEQLESIEQPMERAARALEEGDPLEAHKQQEAAADQLRRLRQHLESQRRNS
                     GGGGSEDPGKGTRSNERVEIPGARSKADELAWRRRVLDSMNGAPPEGYQQAVDDYYER
                     LLR"
     gene            complement(10850..12490)
                     /gene="groL"
                     /locus_tag="VFG22_00055"
     CDS             complement(10850..12490)
                     /gene="groL"
                     /locus_tag="VFG22_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211302.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HET6414653.1"
                     /translation="MAAKEIIYDTAARDRILKGVNALANAVKVTLGPKGRNVVIEKSF
                     GAPTVTKDGVTVAKEIELADRFENMGAQMVREVASKTSDIAGDGTTTATVLAQAIIRE
                     GTKMVAAGHNPMEIKRGIDAGVEQVVKELKELSKATKDPKEIAQVGTISANGDRTIGE
                     IIADAMEKVGKEGVITVEEAKGLETELDVVEGMQFDRGYLSPYFVTDSERMVAEIEDP
                     YILIVEKKISNMKDLLPVLEAIARQQKPLIILSEDVEGEALATLVVNKLRGTLQTAAV
                     KAPGFGDRRKAMLQDIAVLTGGTVVSEDLGIKLENVTLNDLGRAKRVTIDKDTTTIVD
                     GAGKKKDISGRIETIRKQIEDTSSDYDREKLQERLAKLVGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGIVPGGGVALIRAQKGLEKLDLSAEQKIGIGILIRAIEEPL
                     RQIAANAGFEGSIVVNEVRNGKGAYGFNAATEEYTDLVKAGVIDPTKVVRSALQNAGS
                     VAGLMLTTEALIAELPKEETPMPGGGHDHGGGMGGMGF"
     gene            complement(12504..12791)
                     /gene="groES"
                     /locus_tag="VFG22_00060"
     CDS             complement(12504..12791)
                     /gene="groES"
                     /locus_tag="VFG22_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HET6414654.1"
                     /translation="MAIRPLHDRILVKRVKEEETTKGGIIIPDTAKEKPIEAKVVAVG
                     TGRILDSGEVRKLAVKKGDLVLFGKYGGTEVKIDGEDHLILREDDILGIIE"
     gene            12977..13222
                     /gene="rpoZ"
                     /locus_tag="VFG22_00065"
     CDS             12977..13222
                     /gene="rpoZ"
                     /locus_tag="VFG22_00065"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HET6414655.1"
                     /translation="MARVTVEDCLEHEENRFALVILAARRTRQLIKGAPALVYSRNRP
                     VVTALREIADKKVYFARPVRSAVEEFIIETKANESTL"
     gene            13287..14912
                     /locus_tag="VFG22_00070"
     CDS             13287..14912
                     /locus_tag="VFG22_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET6414656.1"
                     /translation="MARISIMGPSGRQERQLFRHNSLGRHPRNTHQVLDRVVSKEHCH
                     IELVEDRYVLKDLGSLNGTYVNGERVDLHPLEPGDEITIGSTRIVFEPEERRGGVSLD
                     GLWKDEPREATNRFKIGDTDAGPLIRAKVSILAEKTFFAEELIQDDASLRRDYEKLRA
                     SYEVTRAIELGTSITALCNKILEVAFRLVPADRGVVLLHNEQEILEPVSVRTAEGVDP
                     DQEVIVSKTVLDTVVREKEAVLSHDASADARFQSAQSVIMQGIRSTMCVPLLHNETVL
                     GVMMIDSRLATHAFTEKDLALLETVASQAAVAVQNTLFARKLEQEAVTRERFRRLLSP
                     AIAEQVISGKLEVKKGGELRETTVLFADIRGFTSISESCEPQIVVDALNEYFEKMVEI
                     VFRYEGTLDKFIGDEMMVLFGSPVAHHDDPIRAVRAALEMQAALLDLNARHEAHGLPP
                     FEIGIGINTGEVVAGYIGSSQALEYTVIGDPVNTGSRLCSLAKPGQTLISEGTVSKLG
                     SQFDLEELPAAQVKGKAQPIRVFELLGRSASGK"
     gene            14959..15915
                     /locus_tag="VFG22_00075"
     CDS             14959..15915
                     /locus_tag="VFG22_00075"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HET6414657.1"
                     /translation="MAHLDFEAPLVELEERIEALEALTDSGTPESKAIEAQIDSLRAQ
                     ANALQRELYEDLDVWTKVQLSRHPDRPYFRDYLSFLFEDFVELHGDRGFRDDPAIICG
                     FARFEGEIVAVIGHQKGRTAKDKALRNFGMAHPEGYRKAVRVMGLAERAGRPILTFID
                     TPGAYPGLEAEERGQAEAIGRAIEVMSALRVPVVCTVIGEGGSGGALAIGVANRVLML
                     EYATYSVITPEGCASILWRDGDRSADAAEQLRLLASDAKRLGVVDEVVIEPPGGAHRK
                     PEAAAEHLRERLRHHLDELRPLSADELVNQRYQRFRALGTWR"
     gene            16031..16789
                     /gene="ybgF"
                     /locus_tag="VFG22_00080"
     CDS             16031..16789
                     /gene="ybgF"
                     /locus_tag="VFG22_00080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HET6414658.1"
                     /translation="MGAQSHVSDASDEATLDAELTALRLKVDEQEQELRTVRGQLALA
                     RSEVQELKSEDTFRAQFSREDSGGEALPWAKNERGDPEMDRVLRLDGEVVEERLPTTS
                     DVPDLPPFVAATPAIAATGEAKATVEAEEVSVEGYRRGLSLIREQKFSEALVELDAFS
                     SAHPSHPYADNALYWCGEIHFLRHEYASALRYFERIEKLHPWANKAPDALYRMGQIYL
                     RRGDTARAQAYFEKVREQFPDTAAARLALREDAS"
     gene            16786..17847
                     /locus_tag="VFG22_00085"
     CDS             16786..17847
                     /locus_tag="VFG22_00085"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET6414659.1"
                     /translation="MKLVGWTSMVAVLIAGTAIAQVGDDPGTEKAKVEVPADMPETHA
                     VKEGDTLWDITETYYGDPYRWPQVWSYNPDITNPHWIYPDATVRLRPGDDEVVKTTPT
                     GFKRTKHQGASTQAVYQAEYGFLDSNALGDAGYIIAANEEHMMMADTDIVYVRFGEGF
                     EPEVGHVYTIFRTIKKSEREASEKGTLVRIMGEVKLQDYDMARRVGHAEIVSTLEPIE
                     RGFRLAPLERHFYWVDVVPAEQSLRATIVASLYPRKLPSTNQVVFVDIGAEQGVVIGN
                     RFYVVRQGDEWRRSAEGKIGREIETTVALPKAPEHYPWEVVAIGRAVNVQPKTTAILL
                     ERANRTVRMGDRAELRKGE"
     gene            17905..19719
                     /locus_tag="VFG22_00090"
     CDS             17905..19719
                     /locus_tag="VFG22_00090"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HET6414660.1"
                     /translation="MASDGDRDGQEALRLQNVVDRERAKLRALQDISAALGSTLDLGE
                     LLEMILDRISEVMEADRSTLYLLDEDTDELWSKVVQGEEVYEIRLKPGEGLAGAVAST
                     GKPLNIKDAYQDVRFDAAWDRATGYRTRSTLCVPMKNQHGRTIGVVQVLNKHEGYFTV
                     ADEALLTALASHAAVSIENSKLFLSVVGKNIELLETKEKLEKKIRELDVLFEITQVAA
                     SAMELDDLLAGVLARTMRATDAEAASILLADEYTGDLTFRAAVGGEPDRVKKMKIKAG
                     QGISGWVAENGRPQIVNRVDDDPRHSAHISDEVGYHPKSVLCVPLRWDEGAGALQLLN
                     KSGGRAPFIDDDLKMATVIAGHLSTAIAQAQSRAQKARQERLSTLGRFLSSMLHDLKT
                     PLTVISGYTQMLVQEPDEGKRREAAASIRRQVQLLKTMMGETLAFAKGERRILIRRVY
                     LHSFFDDLVEQLQPELAERGIRLELVLNDRGVAHFDEEKMLRVFHNLARNAAEAIGDK
                     GGVCRLEVDRTEDGNLVLAFSDDGPGVSDEIRGRLFESFTTHGKQGGTGLGLAIVQSI
                     VEDHGGSVSVDSEPGRTAFRIVLPQPQLRTSTRPAAAE"
     gene            19808..21094
                     /locus_tag="VFG22_00095"
     CDS             19808..21094
                     /locus_tag="VFG22_00095"
                     /inference="COORDINATES: protein motif:HMM:NF023330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 domain-containing protein"
                     /protein_id="HET6414661.1"
                     /translation="MTTENVLDVHPRREELYEELHSRPSPLVETPCSISHIAVQLGQA
                     ERAEECSHLAELCARFEVDPPSDGASCFYKTFDGFEVRWEKHTEFSTYTFIRRTLGDP
                     LRQPSALSLIPEDWLRQIPGKATTALHLAFSCADGALDPSDLDRYFEKQSLRGGLVRQ
                     GTAYVYSSFRLHSDGFGRIVLQSQGLSALQAGRLVQRVLEVETYRVMALLGLPVARKV
                     TPEVTAMEAELAGINQELAQEQDEPGQRALLDRLSNLAARVERHRSDTTYRFAATNAY
                     YDLVRERLGELGEEKVPGLQPVKVFIERRLAPGIRTCNAVRDRLEGLSSRIGHASDLL
                     RTRVELAIQGQNRELLSSMNRRSEIQLRLQQAVEGISVVAITYYLMGLLGYVYEGVAY
                     TGLPLNKKVGFAIAVPLVMFLVWRVIHRVKRRIQQE"
     gene            complement(21104..21466)
                     /locus_tag="VFG22_00100"
     CDS             complement(21104..21466)
                     /locus_tag="VFG22_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HET6414662.1"
                     /translation="MKKTELKRFRAILEEKRDGVIRRARETMDQDMTLDAAELPDEMD
                     LASSEYMQSFTFRLRGRERVFLQKIEKAIKKLDEGVFGICEDCEEPIGSKRLEARPET
                     ELCIRCKEDQERQERDFA"
     gene            complement(21562..22809)
                     /gene="glp"
                     /locus_tag="VFG22_00105"
     CDS             complement(21562..22809)
                     /gene="glp"
                     /locus_tag="VFG22_00105"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HET6414663.1"
                     /translation="MRSIAEALASMLPAFSPLGEEEVHLTESSDRYLSRDLEARLDSP
                     PFDNSAMDGYAVRASDVASAEPDAPVRLPVQGESRAGGPLPGYLEPGAAYRIFTGAPM
                     PAGATAVVIQEDTDSDADGVLIREASHQGKHVRARGSDVRAGTVLLRAGARIGPGEIG
                     LLASQNIARVQVYRRPKVALLSTGDELRALGDPLEPGAIVNSNLYVLTDMLHRLGAIP
                     VALPAARDTLPEIESALRTALEADVVITMGGVSVGAYDFVQEAFKNVGIEPRFWKVRI
                     KPGKPLAFAQHAGKPVVGLPGNPISAMVTFEILVAPCLRKMLGDPQPHPLPVLARLRD
                     AYTRRPGRVEIARAVATREGDEIIVALHGQQGSGSVPSFVGINALVILPSDATELAPG
                     TQVEAILWGSGLMGSGSPFDTIA"
     gene            complement(22847..23734)
                     /locus_tag="VFG22_00110"
     CDS             complement(22847..23734)
                     /locus_tag="VFG22_00110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET6414664.1"
                     /translation="MESEIKTRVAPFSAPHIATGAGQDCLVVIYAPGASQLGQRFSLV
                     DGLARIGRGMENDVVLLSDSVSRRHARIEIRAGSYHLIDTCSTNGTYVNDELVRDRQL
                     RRGDQIKVGDTILKFLSGADLESQYHETIYRMTIVDGLTGIYNKRYLVEQLDRELSRA
                     TRHQRPLTLVICDIDHFKRVNDEFGHLAGDHVLKEVAQLAKSRIRPDDVIARYGGEEL
                     ALILPETDLAGGVRIAGELRQMIASQTFVFEDEDIDVTISCGVAQLEPTWRSHDFVRA
                     ADARLYEAKRAGRNRVAPD"
     gene            complement(23771..24328)
                     /locus_tag="VFG22_00115"
     CDS             complement(23771..24328)
                     /locus_tag="VFG22_00115"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET6414665.1"
                     /translation="MPSPTRITRLALLVYAPLTVGALLWAWGSDRRLAWALETPWMGG
                     SYALRLLVSLGLGLVLAVAVVAMTPVFVERARWARALHEELAEMIHVLSPSEIMVLAL
                     SSGIAEELFFRGAMQPVLGLLLSSAIFGLVHVGPRKVFLAWIAWAFVMGLLFGLIFEL
                     TGVLWGPVLAHVWINQRNMTYIRRR"
     gene            24432..25961
                     /locus_tag="VFG22_00120"
     CDS             24432..25961
                     /locus_tag="VFG22_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET6414666.1"
                     /translation="MSSPPADDVAKILVVDDEEVIREILADFLTMEGFSVRTAKDGGD
                     ALVELSRDRFDLVLSDLKMPNMGGIELLGAIRKHTPHVVTIIMTGFGTVETAIEAMKQ
                     GAYDYVLKPFKVEEVVHTIRRALETQRLEAENFRLKEALSLYKVSEAIAASLSLEQVV
                     HTLSRAAIDEVDSDLVTVLLSDAAGSFYRRSTELNPNFRLRREPDELSIPALTEHFVH
                     APALLEHTQRCWRYFESVAEDPAPQSLAVARLTARGETIGFLCALSYTQGKRFDEGQR
                     KLLHATADRASAAIENARLYEDLQATFKQTIRGLASAIDKMDRYTAGHSARVAAYAQI
                     LAIKLGLGDAEIEVVRQSALMHDIGKIGCVMNLNKPGKLSQEEYEIFKEHPEHGRDIL
                     QPIEFLHPLIPGVHLHHERWDGLGYPLGLKGNDVPLVARIIAVADTYDAMTSDRAYRK
                     AMSHDIAISEIDRCGGSQFDPELADGFLRSIEDFRVEGRSLEEFPDVRETRSGLMAPR
                     R"
     gene            complement(25942..>26272)
                     /locus_tag="VFG22_00125"
     CDS             complement(25942..>26272)
                     /locus_tag="VFG22_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414667.1"
                     /translation="YDSEHGRLVTTTRSFRNENTIRRLVSEGLGDLDYADPAIGPNQT
                     LTLVSVTHLPIREMRLGSLTLVTIFKVGDQPPIVRRWSATPRSRHWNAAFAMPEPPSG
                     AVTSVAP"
BASE COUNT         5034 a         8186 c         8171 g         4881 t
ORIGIN      
        1 cattgaggtt cgatggatcg gccatcgcaa cgatcagcga gctgcccgcg cgggacacgg
       61 gaatgatctt gtgacgttcg caaacctggg gactgatcag cttgagaatt tcccggtcga
      121 tctcgtattc cgaaagatcg atcgactgga cgcgatattg ctcgctcagg aagtcggtga
      181 tctcctgatc ggaaatcgcg cctagacggg cgagggcgta cccgagactg acgttgtcgt
      241 ccttctgctg cttctgcgcc tcgcggagct gctcgaggct gattctcttc tcgcgtacga
      301 ggagctcacc cagtctgttt ctgctcatac ttgttacttc cccaacaaga tccagtctta
      361 gtatatttat tcacaaaata cgagaaagct caagtcccgg accgaggcaa gccgtcggcg
      421 aggcggcgaa tgcgcatcag gtgattatgc gaggtagctc acgttcggaa aaggccattt
      481 ccgaaattgc caatttcctt tgagtttatg gggagttctc cggggccgcg agaccctctt
      541 ggcttgccgc agagcggccg tcgcggtacc gagcgggagt ggcgaagcca aaaaagcaaa
      601 tcactgcggc gccgaaagac accgcagagg ccctcgagca gatagaacag cccaagatga
      661 actggaaggt gctcggcctc ataggcctgg cgctggcggt cctctgggtc atcgcaggca
      721 ttcttcagcc caccatcggc tacgtgggcc tgatcgtggc gggcgtgctt accctggtcg
      781 cgctggggtt cttgatttgg gtttggcgcc tcacgcggaa atcggcggat atcgccgcga
      841 tcctgaaggg cgcaaccgac aaagagggcc gattggccgc gctcgagcaa cttcgggcac
      901 gacagttgcc agggggcaaa gacgcgctca acgccatcgc gcaggcacag ctggtagcgc
      961 aggaagatcc agcggaggcc attcggattc tcgaagggat cgagatcaac aaggcgccgg
     1021 caatgatcca agatgacgtc cgagcgaacc tcgcgatgtt ctacttgatg acgggtcgtg
     1081 cgcgcgacgc ccgcgagctc gcgaacgaaa tccgcctcga ccgccaaccg caagcgaagg
     1141 caaaagccat gtacgcctcg gtcgtagcgg agtcgtacgc gcgaaccgga gacgcggagg
     1201 aagcacgcaa actactcgag acctacgatc cgaaggacaa ggagttcggc caagtctccg
     1261 ggatgctctt gcgcgcgcag gtctacacgt tctccgcaac gaagaagcga ggccgcgcgc
     1321 gcgaggcgat ggagcagctt ctcgaggtgg acccgaacat ggtcgctgcg ttcgtgcaga
     1381 agggaacacg accggagctc agccgcttgg cgaagcaagt gctggcgaat gccggtgcac
     1441 taccaaaacc aaagatgcgc gtgcgcacgc gatagatctc ggtgacctca cgaacttgga
     1501 ccatccgcga agtcctcgag tggaccgcga aggacttcgc ctcgcgaggg atcacgagcg
     1561 cgcgtctcga tgcggagtta ctcgtcgcga aggcgctcga aatggaccga gtggggctgt
     1621 acctcgactt gaaccgcccc ctcgacgaga tcgagcgagg cgagattcgc ctcctggttg
     1681 cacgacgccg agagagggag ccggtcgcct acattctggg ccatcgagat ttctacggac
     1741 gtcgattcgt cgtgactgcg gacgtgctga ttcccaggcc cgacaccgaa accctggcag
     1801 agcgcgcgct cgaatgcatt ccgaaggttc tcgggtgccg agtgctagac gtgggaactg
     1861 gcagcggcgc gctcgccatc acgattgcag ccgagcggcc cttggcggac gtgaccgcca
     1921 ccgacatctc tacggccgcc ttggaagtcg cctccaagaa cgcccgtcga ctcgaggtcg
     1981 ctgaccgaat tcaattccaa tgcactgatc tcctcgaaag cggtcatcag tatgacgtca
     2041 tcgtctcgaa cccaccgtac atcacccgtc gagacatgga gactctgccg gccgacgtcc
     2101 ggcaatacga gcctaggctt gccctggaag cgggcgaaga tgggctggat gtcgtcagag
     2161 cgctgctcgc cgcttgcgag cctgcggcgc agcagggttc gcagttgctg attgaaattg
     2221 gggcagggca ggctgccagc gtgctcgaca tcgcgaaatc gcggagctcg tgggagctcg
     2281 ttgccgtcta cccggacctc aaccgcatcg agcgcgtggt tcacctgcgc cggatctgac
     2341 gcagccagag ctccgtgaac acgagcagag cggcgagcag aagcagccag ggcgctagtg
     2401 gctgctcgac tcccgcggat gcctcggaat cgaccgcctc gatgctgctc tcagcgtcgg
     2461 gcggtccttt cgagaccaag tcactctcgc ccacaggagc atcgacgacg aacgcgaacc
     2521 ttgccccgtt gaccccgttc acgagcacgc ggtacgcgcc gatttcgccc gtgtctcgga
     2581 acgccacgcg gtcggcttcg gccagatcca gcgtccgccc gctcggagtc acgacctgaa
     2641 tgcgttgggt cgatgtcgat cccggcaggg acacgggcgc atagggctcc acggaatgat
     2701 cggatcggcc agcgagccgc gccaaggtgt cgaggacgaa cgggacgaag accggctgat
     2761 acgggagatc ggtccacgcg tcgtcgacgg tcgtcgcgaa catggcaacg cggccactgc
     2821 gataccggtg ctccaacatc gctggcgagc catcgctgaa ctgggccgtg accaccgcgt
     2881 cgagcgaggc ctccgcgatc ggggtgcgac ccatgacctt tgcctggtcg agctccggca
     2941 aggactcgga ctcgagcgac aaaggactct cctccattcg ggtggcggtg taccgccctg
     3001 gaaggagctc tcggagcacg ctcccgtacg cgcgaggatt gatccggtca cccgcggtga
     3061 tccacaggct ccctccaccg ctcacgaacc gctgaatcag tggcagccac ttctcgtcga
     3121 gtgtggcatt ggccaacacg aggacgtccg agcgaatgat cgcgctttcc gaaaggtcct
     3181 gagcgtcgag tgtttcgact gcgaacgagc ggccaacgcg gggtgcaacg ctgagcgcgt
     3241 gattgagaaa cgccgtttcc cgcttgcgcg gatcggggtg aggatcgccg tcgacgagca
     3301 agacatgcac ggtgccggag ccgagaagaa cgcctcgtac gttgtcagca ggcaagacgt
     3361 cgtcaggatc cagctgcaat tcgactactg cgtctgtctg gccagctttg atgaagagca
     3421 cggcttctcc gccagcctcg gtccgttcga cggttgcctt ttgcgtttct tgccctttct
     3481 gccgcaagcg cacccgttgc tgcaccgcgc tcggtggacc cacgatctcc acttcgaggc
     3541 gcaggccttc cgcggcatcg ttctcgccga gcacatcgac ggatccgatg cggcgatttt
     3601 ccggattggg agtagaaacc gtttcgaatc cgacgcgaat gccacgcgtc ggccattcgc
     3661 actcgtccgc acgcgcgccg ggcgtgaagt ccgagagcac gagcaacctt cgatccggca
     3721 gctcgctttc gagcagcagc tcgacggcgg cctgtacagc gtcacagagc gcgttgcctc
     3781 gggtcgagcg cgtgtcgatt tcgccaaaga cggtctccac catctcgagc tcggaacgct
     3841 tgcgcagcca gacccgcgct ggtttgcccg agagcaccac agagacttcc gagccctcgg
     3901 gtaggcttcg aacttgctca gccgccaggg cggctgcttg ctcgatcagg gtctcgccct
     3961 cgtgaatggc cgccatcgac agggagtcat cgaggacgat ggcgagcgca tgctgccggt
     4021 ccaacaagct cgagtgaacg acccggtagg gcgccgccgc gacgagcgca aggagcgcca
     4081 ccagcaaggt ccgcacgatc agcaggagca tgtccaccac gcgcaagcgc gccgcccgcg
     4141 tagcgtggat tcgatgcagg aatgcgatcg ttggaagagt ctgcctcggc agcttttgac
     4201 gcctcagcag gtgcgcgagg atcggaatcc cgaccgcgat gagaccagcg agtgcccacg
     4261 gaaccccgaa ccccatggct cagccatccc tcagcagctg aagcagagtg tgctcgggag
     4321 gtgtgtctgt ccttgcaaga agataccgcc cgcccgccgc cacgacccta tgttggcacg
     4381 aggccacgaa ggcatcgatc gactccttgt agcggcgccg gatccctttc gggtccacct
     4441 ccaccggcaa ttcaccttcg agtccctcga agtgcatcgg ttgctcatac ggaagctcga
     4501 gctcggccgg gtccatgacg tgtagaacga cggtttggtg acccttggcc tgaatcctag
     4561 ccaccggatc gagggcgtcc tgctcgaggt cgagcaaatc acttgcgatc accacgagcc
     4621 ccattcggcc cgcatgctcg atcagcttgt cgatcgacgc gtgtagcgca gtggcgcccg
     4681 tagacggcga gggggccgcc aaggtctgca tcaggtagtc gaggtgcgca ggccccgagc
     4741 gagccggcag ccggtctcgg atggcgtcgt cgaaggtgat gagggacacc gcatcgcctt
     4801 gtcgcgtcag gacgtaggca agggcggcga gcaaggtcgc tgcatggtcc agcttgctga
     4861 cgtctccgct tccccagcgc atcgagcgtg aaatgtcgag cgcaagcgtt ccacggagct
     4921 gagtctcgtg ctcgaagtgt ttgatgctgt agcgatccga gcgggcgtag gcgcgccagt
     4981 cgatgagacg gggatcgtcg ccgggtcgat acgcccgatg ttctttgaaa atcgcgctcg
     5041 caccacgatg tggactccgg tgcatacccg caaggagccc gtcgaccgta cgctttgctc
     5101 gaagcgtgag cccggccaag cgtaccgcga gctcgggttc gagcacgtgg gtcacggctc
     5161 gatgctttgc ttgatgcttt cgattacgtc tgcggaagtc acgccttccg cctccgcacg
     5221 gaagttcatg acgagacgat gcgtcaggat gggctctacc agcgcgcgca cgtcttggat
     5281 ctccgctgca tagcgaccgt gtagaattgc acgagccttc gcacccagaa tcagcccctg
     5341 gctcgcccgg ggacccgctc caaaggcgac gttcttccgg acgagctcgg aagctctcgg
     5401 gtcttccggg cgcgaggccc gcaccaacgt aaccgcgtgc tgaatcacgt gatccgcggc
     5461 aggaacccgc ggaacgaggt cctgaaagcc gacgatgtcc tgcgcagaga ggacgggggt
     5521 gatcgacgtg ggcatcggcg cggtagtgcg cttcacgatg tcgatctcct cgtgttgact
     5581 cgggtaccga acgtcgatct gaaagaggaa ccgatccagc tgagcctcgg gcagagggta
     5641 ggtcccttct tgctcgatcg gattctgcgt cgcgaatacg aggtacggtt gctcgagagc
     5701 gtaggtgttg ccgctggccg tgacctgccc ctcagccatg gactgcaaga gcgcggcttg
     5761 cgtcttcggt ggcgcgcggt tgatctcgtc ggccaggaga atgttcgtga acaccggacc
     5821 cgggatgaaa cggaagtggc gctttcccga cgtggcatcc tcttcgagca cgtcggttcc
     5881 ggtcacatcg ctcggcatca ggtcgggcgt gaactggatg cgattcgaat gaagcgagag
     5941 ggcatcggca agagtcgtga tgagaagcgt cttggccaaa ccgggcacac cgacgaacaa
     6001 gccatggccc cgcgcgaaca gggcgatcag gaggagctcg atcacgtctt cttggccgac
     6061 gatgcgctgc ttgaggcgcg agatgatctc gtcgcgggca tcggaaagct gctgcacgcg
     6121 cagaacgtcg gatgtggcac catttggtgc ggcgggcgga gaactgcgga cgggttcgct
     6181 cacgaggacc tcttagcgcc tgctcgaccg aattgccaca ttactcccgc atgcctccga
     6241 gcctcgagca caaagtcgct tcgtgctcgg cttggggtag tgggagcttt gaaaggatgc
     6301 agtgaggctg cggatcgaac ggattgtgag caatagcctc gttggcggca tccctcgcgc
     6361 ccccaaaatt cgacatgcgg aaaagcgcgg tcgcgaggct cgacaatgct ggtgcgtgtg
     6421 ttgggtaccg ttctagggtt cgcttgagcg gcgcgatcgc gttttggggt cgccctccgg
     6481 cgatcgctga acgcgcgtag cgtgaagcaa cgactggatc cgctggagcg gcctcgtgtg
     6541 ctttcgcata ctcgaccgac gccgctgcgg gccgagaacg agtccacaag agatcaccca
     6601 agcgaacata gttgcgggcg cgatccagct cgaccccggc aatctcgtct tgttcggttg
     6661 cttcgccgct gaggtaaagg ggcattagtc tcgcaggcgg agagtccggg tcgctcgaca
     6721 agctgctcct ccagcgcgtc tctagggtct tccacgaaac gcctgccacg cgagccaacg
     6781 ccttgcgtgc gtcttcgccg gcaccgacgc gctcgagcgc tttctgaact ccatctcgtc
     6841 cgaactcccg gtagtacatc gccatgaacg aagagacctg tgcgaaggcg agggcggcca
     6901 ccttctgtgt cggcaggagc gcgatggacg gatgcatttg atcgaagtcg agcagctggt
     6961 ccgcacgggc cgcttcatga agcaactggg tcgccgcggg gtcatgcctg aggcttgcct
     7021 cgtcctgacg ccagcgggtc tcgagaaact tggctagccc ttcctgaagc cagacgggtg
     7081 cacggtcgag agaagccttc gtcaggagca ggtgcacgta ctcgtgtacg atggtgtcca
     7141 gccagggata accgtgcatg agcgcgcctg gcgaagcgat catcaatcga ccccacttgc
     7201 acaaggcgat cgtgccggtg tttcgaatgg catcgacaga caaggtcgac acgcgcgcca
     7261 aggactccgc gtccgggtaa atctcgaggc ggactgggct gaccatcttg accccgagct
     7321 ctgcctctag cgcctccgtg gccgctttga gcgcgtcgat ggcgtacgga accaggatct
     7381 cgtcgtctcc gggcgcgtaa cgcacttcga agttctcgta cacgacggtc tcgtagttct
     7441 tcgtcgtctc gtatgtgtcg acgatgacgt cgacgagatc ccccagttcg gtgccgtagc
     7501 gcggatcctt gcgcagctcc gcgattccgt tggctgcggc ctcgtagtcc ccggaataga
     7561 actccgcaag aacttcgagc tccatcacgc gcgaggtcct aggaaactgt tctacatact
     7621 tccgcaccaa aatctgggcg ggcacacctt cgccggcagc aagagaggct tccaagaggt
     7681 cgaggacctc cgagctcgtg gcgttctcgg ctggcgcgga cgtcgctagc gagccgagca
     7741 acaaacagca cgcaaacaag ggcagccgat tcatcggaga agcctttcgt agtagtcgtc
     7801 taccgcttgt tggtagccct cgggaggagc gccgttcatc gagtctagga ctcgccgacg
     7861 ccaagcgagc tcgtccgctt ttgaacgcgc gcctgggatc tcgacccttt cgttagaccg
     7921 agtgcccttg cccgggtcct cacttccgcc cccgccagag tttctccgct gcgattcgag
     7981 atgctgccgc aatcggcgca attgatccgc agcagcctcc tgctgcttgt gcgcttcgag
     8041 gggatctcct tcttccaatg cgcgcgcggc tcgctccatt ggctgctcga tcgactccag
     8101 ttgctcggcc gcttcctcgg aaacgggtac accgccgact tcttcgcgaa gccggcgagc
     8161 cagctgatgg gtcgcctctt tcgcgtctcc ctgctgggcc gcctgctttg cgagctcctg
     8221 ccgctctccc atcgtcagtg cattcgcgat gtcgggaagc gcaccctgtg cggcgtcccg
     8281 caggtcctgg gcgcgagcag caacctgccg tgcctgcgca gccgcctcgg ccgtctgccc
     8341 gccgcggccg cgaaacatca tggcggagag ctcgagatct ttcgcgagtt ggtcggccgc
     8401 ttgtgccaaa cggtttgcca tcgacagggc ttcgccgagg tcggctgctt cgagtgcctt
     8461 ctggaaatcg ctcagacgtt ccgatgcccg tccctggagg ctggactcgt agggcccgag
     8521 cgcctcgtcg ttgagcccgc cgagtagctc cgagacctct tttgcgatct tctcgagctc
     8581 accgcggatc tcggacgcca ttgacttctt gtcgcccgag acacgatcca ccgccttgtt
     8641 gcctgctcgg cgcgctttgt cggccatccg cttctgctcg acctcgaggt cccgaatcgt
     8701 atctgtcgcc ttcatcagcg cccgttcgcg tgggccgaag cgggcctcga cgagggagtc
     8761 ctcgctctgc gcgagcgcct gcgtcatcgc gtcgatcgtt tgatccagct tggtcaatgc
     8821 ggcctcggcg gcgtcgagat ctcccgcagc cagtgcttcg cgaacacttc gcagcgcatc
     8881 cgtcgacttg ttcgcctgct gcgcttgtcg attgacgaac tcgccgggaa cgtactgcat
     8941 cgcctgccga agctggtctc gcaaagctcc catgcgccgc tcggcccgat ccagcgcagc
     9001 catgagcgca cgctgcagct ctggagtctg accgcggcgc agctcgccga tgagcgatgc
     9061 aatttcttgt cgcagttgct gcatctctcg ggcgatctcc gctgcgtcgt tgagacgggc
     9121 ttcactgatc aggtccgcta cgatcaaagt gtccttctcg agctcgctca caatcgcctt
     9181 gtcgaagcga tgccggttgc tgacctgtgc tgccgaaagg tacgctgagt cctcgcgtct
     9241 gcctgcgcgc tcgagacgct ttctcatgtc gagcaaaagc ggaaccgttc gtggtccgcg
     9301 atgatctcct acgttggcga gcaacgacat gacaccccgg tacggaacca tgctcgcatc
     9361 ccggcggcgc cgcgcctcgt cgagcctctt gggcagaggt tgttccagac gcactgccag
     9421 tgccgagagg accgtgttga gcagctgttc gagctcgact attcgatccc ggcgtcgcgt
     9481 gatctccgaa gcgagagtga gtgttcgcac ggcggaacgc ccgattcctg gaccgtcgac
     9541 tcgattggca tccttggctt ccaaccagat ggtcaccgca tctgcgggcg aaagagagaa
     9601 ctcctccaag cgtaacgccg tttctccgta gaaatcgttc ttcgtctctt gcgatggcgc
     9661 gaggatcgcc ctgcgcaaat gctcgccatt ggggagcttg atgatcagcg tgagctcctg
     9721 aaccccgtaa tcgtcggtgg cactgtgttc gagaagaacg ggttgcctct cctcgacgac
     9781 caggtcgtct tctggagcga tgagggtcac ccgcggcggc tggtcaactt gaaccgatat
     9841 ggagcgagcg cgatggtcca ctcggcgttc gccgcgcgtg gattcgacgt aaatctcgag
     9901 aggcccgtcg gtatcggcga cgaacgatgc gacgaattgc tcgccggcgc gctttgcttc
     9961 tacatggcgt cccggaagct ccacgaccac gcgcgcggca tcgccgatcg gagtgatggc
    10021 gtattcgatc gctgttcccc gaggtacgat gaggcgatct gcctcttcga tacgctcgtt
    10081 gacccgttgc atgtactccg ggaaaaacag atccgcgccc gtgtgcgcca ctacatggct
    10141 ggttcggaca ccttcgactt ctccgaggtc cgcgtgcaga agagcatagc ggccggcagt
    10201 tggacgatcg aagtcgaggg accaccaggc agccgcagcg agtatcagtg ccgccgccaa
    10261 cgccggttga actagccaac gccatgggat gaatttgcgc gcaggggagg cgtcgagcgc
    10321 acgcaagagc ccaagtcgct gagcgatgac cagctcccgg gaaaacgcct gctcatcctg
    10381 aagctcgtag gcgctctgca ggggagacaa gagggaaggt tgccccgtgg ccacaagccc
    10441 gagcgcgcca tgcccgcgaa gcttgaccag cggctgaatc gaccatgcca ccgcccccgt
    10501 ggtcatgccg aaaaccacgc tccagccgat gcagagccaa acgactgggg tcatgggtcc
    10561 cagcgcccat gcgacgatga cgaacgaggc gaggcccacc gcggctgcgg ctgctgagat
    10621 tctgagagcc atcacggtag ccgccgcgag ccgaaggttt cgccggtact cggccagtct
    10681 gcccgtggtg gagctcacgc agcgatcgta gcgcccagcc ccccgtcggc gccaccccct
    10741 tcactcgcac tgtcaacctc agcgccagtg aacggcgatc gcgcttggct taaacggaaa
    10801 agccggagca caaatgctcc ggctcttcag tgttgaggcg agcgctggct tagaagccca
    10861 tgccgcccat gccgccgccg tggtcgtgac cgccgccggg cattggggtc tcttccttcg
    10921 gtagctccgc gatcaaggcc tcggtggtga gcatgaggcc cgcaaccgaa cctgcgttct
    10981 gaagcgcgct acgcacgacc ttggtcgggt cgatgacgcc agccttgacg agatcggtgt
    11041 actcctcggt cgcagcattg aacccatagg cgcccttgcc gttgcggacc tcgttcacga
    11101 cgatcgagcc ttcgaatcct gcgttggcag cgatctgccg aagcggctct tcgatggcgc
    11161 ggatcagaat gccgatgccg atcttctgct cggcgctcag atcgagcttc tcgaggccct
    11221 tctgcgcgcg aatcaacgca acaccaccgc cgggcacgat gccctcttcg acggcagcgc
    11281 gcgtggcgtg gagcgcatct tcgacgcgcg ctttcttttc tttcatctcg acctcggtgg
    11341 ctgcgccaac cttgatcacc gcgacgccgc ccacgagctt ggcgaggcgt tcctgcagct
    11401 tctcacgatc gtaatcgctg ctggtgtcct cgatctgctt gcggatggtc tcgatgcggc
    11461 ccgagatgtc cttcttcttg ccagcgccgt cgacgatggt ggtggtgtcc ttgtcgatcg
    11521 tgacccgctt ggcccgaccg aggtcgttca gcgtgacgtt ctcgagcttg atgccgaggt
    11581 cctcgctcac gaccgttcca cccgtgagga cggcgatgtc ctggagcatg gccttgcggc
    11641 gatcaccaaa gcccggagcc ttgacggcgg cggtctgcag tgtgccacgc agcttgttca
    11701 cgacgagcgt ggcaagtgcc tcgccttcga cgtcctccga gaggatgatg agcggcttct
    11761 gctgacgggc aatcgcctcg agaaccggca gaaggtcctt catgttcgag atcttcttct
    11821 cgacgatgag gatgtatgga tcttcgatct cggcaaccat gcgctccgaa tcggtcacga
    11881 agtagggcga caggtaaccg cggtcgaact gcataccctc gacgacgtcg agctccgtct
    11941 cgagaccctt ggcctcttcg acggtgatga cgccttcctt gccgaccttt tccatcgcat
    12001 cggcgatgat ttcaccaatg gtgcggtcgc cgttggcgct gatcgttcca acctgtgcga
    12061 tctctttcgg gtccttcgtg gccttcgaaa gctccttgag ctctttgacg acctgttcga
    12121 cgcccgcatc gatcccacgc ttgatctcca tcgggttgtg ccccgcggcc accatcttgg
    12181 tgccttcgcg gatgatcgcc tgggcgagca cggtggcggt ggtggttcca tcgcccgcga
    12241 tgtcggaggt tttcgaggcc acctcgcgca ccatctgtgc gcccatgttc tcgaacctgt
    12301 cggcaagctc gatttccttg gcgaccgtga caccgtcctt ggtgacggtc ggtgcgccaa
    12361 aacttttctc gatcacgacg ttgcggccct tgggaccgag cgtgactttc acggcattcg
    12421 ccagcgcatt gacgcctttc agaatgcggt cgcgagcagc ggtatcgtaa atgatttctt
    12481 tcgcagccat gtttctcagt ctcctattcg atgattccga ggatgtcgtc ttcgcggagg
    12541 atcaggtggt cttcgccatc gattttgacc tccgtgccgc cgtacttgcc gaacaggacg
    12601 aggtcgccct tcttcacggc aagcttccgg acctctccgc tgtcgaggat gcgcccggta
    12661 cccaccgcga ccaccttggc ctcgatcggc ttctctttgg cggtatccgg aatgatgatc
    12721 ccgcctttag ttgtctcttc ttccttgact cgcttgacca gtatccggtc atgcaatggt
    12781 cgaatggcca tttatccctc cgtgttcggg cagctactcg gcccgctagc ttgattagca
    12841 ctcgatgatg gagagtgctg atgggccgcc aacctaatcc tgaattgtag cgagtcaagc
    12901 catggttccc gggtttctta gaccgacggg tgactgattt gccgcgccct cagacccctg
    12961 ctagcgtccg ccccctatgg cacgcgtaac ggtcgaagac tgcttggaac acgaagaaaa
    13021 ccgctttgcg ctggtcattt tggccgcacg gcggacccga cagctcatca agggcgcgcc
    13081 ggccttggtt tacagccgaa accgccctgt cgtgaccgcg ctgcgtgaaa tcgccgataa
    13141 gaaggtgtat ttcgcccgcc cggtgagaag cgccgtcgaa gagtttatca tcgagaccaa
    13201 ggcgaacgaa tccacgctct gacgcggcgc tgagctccct caactttgca gcctgatgag
    13261 gccgcaggta gcctgatggt ggggtcatgg ctcgcatcag catcatgggg ccaagcggcc
    13321 ggcaggagcg tcagctcttt cggcacaact cgcttgggcg tcatccacgg aacactcacc
    13381 aggttctcga ccgggtggtg tcgaaggagc actgccatat cgagctcgtg gaggaccgct
    13441 atgtcctcaa ggatctcgga tcgctgaacg gcacctacgt gaacggcgag cgggtcgacc
    13501 tgcacccgct cgagccaggg gatgagatca ccatcggctc gacccgtatc gtcttcgagc
    13561 ccgaggagcg gcgcggcggc gtctcactcg atgggctctg gaaggacgag ccccgggaag
    13621 cgaccaatcg gttcaagatc ggcgacacag acgcgggacc cttgatccgt gcgaaggttt
    13681 cgatactcgc cgaaaagacc ttcttcgccg aagagctgat ccaggacgac gcatcgctgc
    13741 gacgtgatta cgagaagctt cgagcaagct acgaggtcac gcgcgcgatc gagctcggca
    13801 cgagcatcac agcactctgc aacaagatcc tcgaggtcgc tttccgtctc gtgcccgccg
    13861 atcgcggcgt cgtgcttctg cacaacgagc aggagattct cgaacccgtg tcggtgcgca
    13921 ccgcggaggg tgtcgatccc gatcaggagg tcatcgtctc gaagaccgtg ctcgacacgg
    13981 tcgtgcgtga aaaagaggcc gttctttcac atgacgcctc ggcggacgcg cggtttcaga
    14041 gcgctcagtc ggtcatcatg cagggcattc gttcgacgat gtgcgtaccg ctcctccaca
    14101 acgaaaccgt tttgggagtg atgatgatcg attcgaggct tgccacacac gcgttcacgg
    14161 aaaaggacct ggctctcttg gaaacggtcg cgagccaggc cgctgttgcc gttcagaata
    14221 cgcttttcgc acgcaagctc gaacaggaag ccgtgacccg ggagcggttc cgacgattac
    14281 tgtctccggc gatcgccgag caggtgattt caggaaagct cgaagtgaag aagggtggcg
    14341 agcttcgcga gacgacggtc ctattcgccg acatccgggg cttcacgagc atcagcgaat
    14401 cctgcgaacc acagatcgtc gtcgacgcgc tcaacgaata cttcgagaag atggtggaaa
    14461 tcgtgttccg ctacgaagga actctcgaca agttcatcgg tgacgaaatg atggtgctct
    14521 tcgggtcgcc cgtcgcgcat cacgatgacc ccattcgcgc ggttcgcgcg gcgctcgaga
    14581 tgcaagcagc gctcctcgac ctgaacgcac ggcatgaagc ccatggctta ccacctttcg
    14641 agatcggaat cgggatcaac accggcgaag tcgtcgcggg ctacattgga agcagccagg
    14701 cgctcgagta caccgtcatc ggggacccgg tgaatacggg ctcccgattg tgctcgctcg
    14761 cgaagccggg acaaaccttg atcagcgagg gaaccgtgag caagcttggc agccagttcg
    14821 atctggagga gcttcccgca gcgcaggtga agggcaaagc ccagccgatt cgggttttcg
    14881 agctgctcgg ccgttccgct tcagggaaat gacccatgcg cttgcccggc aaggcagata
    14941 tgcgaagcat ctcattcgat ggcgcatctg gactttgaag cccccttggt cgagctcgag
    15001 gagcgaatcg aagctctaga ggcgcttacc gactccggca ccccggaatc caaagccatc
    15061 gaggcccaga tcgacagctt gcgcgcccag gccaacgcgc tgcaacgcga gctctacgag
    15121 gatctcgacg tatggaccaa ggtacagttg agccgccacc cggaccgtcc ttatttccga
    15181 gactatttgt cgttcctctt cgaggacttc gtggagctgc acggtgaccg agggtttcgg
    15241 gacgatcctg cgatcatctg tggcttcgcg cgctttgagg gcgaaatcgt tgcggtcata
    15301 ggtcatcaaa agggtagaac cgcgaaggat aaggccttgc ggaacttcgg catggcgcac
    15361 ccggaagggt atcgcaaagc cgtgcgcgtc atggggcttg cggaacgggc cggccgaccg
    15421 atcctcacgt tcatcgacac gcccggcgca tacccagggc tcgaggcgga agagcggggt
    15481 caggccgagg ccataggccg ggccatcgag gtcatgagcg ctctcagggt gccggtcgtc
    15541 tgcaccgtga ttggggaagg cggttccggc ggggcgctag cgattggcgt cgccaaccgg
    15601 gtcttgatgc tggagtacgc cacctatagc gtgatcacgc ccgagggctg tgcctcgatc
    15661 ctttggcgtg acggcgaccg gagcgcggac gccgccgagc agctccggct gctcgccagc
    15721 gatgcaaagc ggctcggggt cgtcgatgaa gtggtgatcg agccgccagg aggcgcgcac
    15781 cggaagcccg aggcggcggc cgagcacctc agggagcgcc tccgccacca tctcgatgag
    15841 cttcgacccc tctccgcaga cgagctggtc aatcagcgat atcagcgatt tcgggcactt
    15901 gggacctggc gataggccac gccttttttg acgtgagtcg gctgggctac ctacgattca
    15961 ggtgcggggc tatgccgtga aatcgctctt cgtagtggct ggtgtgtcaa cactgatcgg
    16021 gtgcagctcg atgggagcgc agtctcacgt cagcgacgcg tccgacgagg cgacgctaga
    16081 tgctgagctc acggcgcttc gactgaaggt cgatgagcaa gagcaggagc tccgaacggt
    16141 gcgcggtcaa ctcgccttgg ctcgctcaga ggtacaggag ctcaagagtg aggacacctt
    16201 ccgcgctcaa tttagtcggg aggactccgg cggtgaggcg ctgccctggg cgaagaacga
    16261 gcggggagac cccgagatgg atcgggtgct tcgtctcgac ggcgaggtgg tcgaggaacg
    16321 actgccgacc acttccgacg tgccggactt gccgccattc gtagccgcga ctccggccat
    16381 agctgcaacg ggcgaagcca aagcgaccgt cgaggctgag gaagtcagtg ttgaaggcta
    16441 tcggcgcggc ttgagcttga ttcgagagca gaagttctcg gaggcgctcg tcgagctcga
    16501 tgccttctca tctgcgcacc cgagccaccc gtacgcggac aatgcccttt attggtgcgg
    16561 cgagattcac tttctgcgcc acgagtacgc ttcggcgctt cgctacttcg agcgaatcga
    16621 gaagctccat ccctgggcaa acaaggcgcc cgacgctctt tatcgcatgg gccagatcta
    16681 tctgaggcgc ggtgatactg ctcgtgctca ggcatacttc gagaaggtta gggagcagtt
    16741 cccggatacc gcggcggcgc gattggcgct gcgggaggac gcatcatgaa gctggtggga
    16801 tggacgagca tggtggccgt gctgatagcg ggcacggcga tcgcacaggt cggcgacgac
    16861 ccagggaccg aaaaggccaa ggtggaggtt cctgcggaca tgccggaaac gcacgcggtg
    16921 aaagaggggg acacgctctg ggacatcacc gagacgtact acggagaccc ctatcgctgg
    16981 ccgcaggtct ggtcatacaa ccccgacatc accaaccctc attggatcta ccccgacgcg
    17041 accgtgcgac tccgcccagg cgatgacgaa gtggtcaaaa ccacaccgac agggttcaag
    17101 cgcacgaagc accaaggcgc atcgacgcaa gcggtgtacc aagccgagta cggtttcctc
    17161 gattcgaacg cgctcggcga tgctggctac atcatcgctg ccaacgaaga gcacatgatg
    17221 atggccgaca ccgacatcgt gtacgtgcgc ttcggcgaag gcttcgagcc cgaggtcggc
    17281 cacgtctaca cgatcttccg aaccatcaag aaatcggaac gcgaagcctc cgagaaggga
    17341 actctcgtgc ggatcatggg ggaggtaaag ctccaggact acgacatggc tcgacgggtc
    17401 ggtcacgccg agatcgtcag caccctcgag cccatcgagc gcggattccg cctcgcaccc
    17461 ctcgagcgcc atttctactg ggtcgacgtc gttccggccg agcagtcgct gcgcgcgacc
    17521 attgtcgcca gcttgtatcc ccgaaagctt ccctccacca atcaggtcgt gttcgtcgac
    17581 attggcgccg aacagggcgt ggtcatcgga aaccgtttct acgtcgtgcg ccaaggcgat
    17641 gaatggcgcc gcagcgccga gggcaagata ggtcgcgaga tcgaaacgac cgtggccctg
    17701 ccaaaggcgc cggagcacta tccctgggaa gtcgtggcga tcggccgagc ggtcaacgtc
    17761 cagcccaaga ccaccgccat tctgctagag cgcgccaacc ggaccgttcg aatgggcgat
    17821 cgggcagaat tgcgcaaagg ggaatagcgc cccctcgaag gtcgttgctt ccaagcccag
    17881 gtttggggga gtatcgaccg gaagatggct tcagacggtg accgtgacgg ccaggaagcg
    17941 ctgcgacttc agaacgtcgt agaccgcgaa agggcgaagc tacgggcgct gcaagacatc
    18001 agcgctgccc tcgggtcgac tctcgacctg ggcgagttgc tcgaaatgat cctcgatcgg
    18061 atctcggaag tcatggaggc ggaccgctcg acgctctact tgctcgacga ggacacagat
    18121 gagctctggt cgaaagtcgt ccagggcgag gaggtctacg agattcggct caagccaggc
    18181 gagggtctgg caggagccgt ggcgagcacc ggcaaacctc tcaacatcaa ggacgcctac
    18241 caagacgtcc gcttcgacgc ggcttgggac cgggcgaccg gttaccggac ccgctcgacc
    18301 ctctgcgtcc cgatgaagaa tcaacacggc cgcaccatcg gagtggttca ggtcctcaac
    18361 aagcacgagg gctacttcac cgtggccgat gaagccctgc tgacagcgct tgcaagccat
    18421 gccgccgtgt ccatcgagaa ttcgaagctg ttcctttcag tggtcggcaa gaacatcgag
    18481 ttgctcgaga ccaaggagaa gctcgaaaag aagattcgcg agctcgatgt tctcttcgag
    18541 atcacccagg tcgcggccag cgcgatggag ctggacgacc ttctggccgg cgtgctcgcg
    18601 cgaaccatgc gcgcgaccga tgcggaggct gcctcgatcc tgcttgcgga cgagtacacg
    18661 ggagacctca cgtttcgcgc agcggtaggc ggagagccgg accgcgtgaa gaagatgaag
    18721 atcaaagccg ggcagggcat ctccggctgg gttgcggaga atgggcgtcc gcagatcgta
    18781 aatcgggtcg acgacgaccc taggcattcg gcccacatct ccgacgaggt gggctaccac
    18841 ccgaagtccg ttctctgtgt gccgctgcgt tgggacgaag gcgccggcgc gctgcaatta
    18901 ctgaacaaat caggcggtcg cgcgccgttc atcgacgacg acctcaagat ggcaacggtg
    18961 atcgccggcc atctttcgac cgcaatcgct caggcgcagt cccgcgccca gaaggcgcgg
    19021 caggaacggc tgtcgacgct cggccggttc ttgtccagca tgctccacga cctcaagacc
    19081 cccctcacgg tcatttcggg ctacacgcag atgctggtcc aagaacccga cgaggggaag
    19141 cggcgcgagg ccgcggcttc gattcgccgt caggtccagc tgctcaagac catgatgggc
    19201 gaaacgctcg cgttcgcgaa gggagagcgg cgaattttaa ttcggcgcgt gtatctgcac
    19261 agctttttcg atgatctcgt cgagcagctc cagcccgagc tggccgagcg cgggattcgc
    19321 ctcgagctgg tcctgaacga ccgcggcgtg gcgcacttcg acgaagagaa gatgctacgc
    19381 gtcttccaca acctcgcacg caatgccgca gaggccatcg gggacaaagg cggggtttgt
    19441 cggctggagg tcgaccgaac cgaagacggc aacctggtgc tggccttctc cgacgatggc
    19501 cctggggttt ccgacgagat tcgcggtcgc ctgttcgagt ccttcacgac ccatggcaag
    19561 caaggtggta cgggattggg actcgcgatc gttcagagca tcgtcgagga tcatggcggg
    19621 agtgtgtcgg tagacagcga gccggggcga acggcgttca gaatcgtctt gccccagccc
    19681 cagctgagga cgagcaccag acccgctgct gccgagtgag ggtttcgact gaggggcggg
    19741 gcgcagacac ttgcgttggc cctcgcccca gttgacccgg ggagccaccc ctccaaagat
    19801 agacccggtg accaccgaga atgttctgga cgtgcacccc aggcgcgaag agctctacga
    19861 ggagcttcac agtcgtcctt ctcccctggt cgagacccct tgctccatca gccacattgc
    19921 ggttcagctc ggccaggctg aacgcgccga agagtgctcc cacctggccg agctctgcgc
    19981 ccggttcgaa gtggacccac cttctgacgg tgcatcctgt ttctacaaaa ccttcgatgg
    20041 tttcgaggtt cgatgggaaa agcacactga attttcgact tataccttca ttcgaaggac
    20101 cctcggcgac cctctaagac agccaagtgc actgtcgctc atcccggagg actggctccg
    20161 ccagattcca ggaaaggcca ccacggcgct gcatctggcc ttcagctgtg cggatggtgc
    20221 cctagaccct tctgacctcg accgctattt cgagaagcag tcgcttcgcg gcgggctggt
    20281 gcggcagggg acggcatacg tctacagcag ctttcggctc cactccgatg gctttggacg
    20341 catcgttctc cagtcccagg gtctcagcgc ccttcaggcc gggcgtttgg tgcagcgtgt
    20401 gctggaagtc gagacctacc gtgtgatggc cctgctcggg ctgcccgtcg cccggaaggt
    20461 gacgccagaa gtcaccgcga tggaggcaga gctggcaggg atcaaccagg agctggccca
    20521 ggagcaagac gagcccggac agcgtgcgct gctggaccgg ctttcaaacc ttgcggcccg
    20581 cgtcgagcga catcgctccg atacgactta ccggtttgcg gccaccaatg cctactacga
    20641 cctggtgcgc gaacgcctcg gtgagctggg cgaggaaaag gtccccggcc tacaacccgt
    20701 gaaggttttc atcgagcgcc gcttggcccc aggaattcgc acctgcaacg cggtccgtga
    20761 ccgtctcgaa ggtctttcct cgcgaattgg ccacgccagc gacctgctcc gtacacgcgt
    20821 cgagctcgcc atccagggcc agaaccgcga gctgctcagc tcgatgaacc gccgaagcga
    20881 aatccagctg cgccttcaac aagcggtgga ggggatttcg gtcgtcgcga tcacctatta
    20941 cttgatgggc ctgcttggct acgtctacga aggggtggcg tacacgggcc taccactaaa
    21001 caagaaagtt ggctttgcga tcgcggtacc gctcgtcatg ttcctcgtct ggcgagtcat
    21061 tcaccgcgtc aagcgccgca tccagcagga atagaggggc cgactacgcg aagtcgcgct
    21121 cttggcgttc ctggtcttct ttgcagcgga tacagagctc ggtctcgggc cgcgcctcga
    21181 gccgcttgct gccgatcggc tcttcgcaat cctcgcagat cccaaaaacg ccctcgtcga
    21241 gcttcttgat ggctttttcg atcttctgaa ggaacacgcg ctcgcggcct cgaagtcgaa
    21301 atgtgaagga ctgcatgtac tcgcttgatg ccaggtccat ctcgtccgga agctcggccg
    21361 catcgagggt catgtcctga tccatggtct cgcgcgcccg gcggatcaca ccgtctcgtt
    21421 tttcttctag gatggcgcga aaccgcttga gctcggtctt cttcatgctt gtgaacccct
    21481 ttgagggagc gcgactatat tgatcaagtc cgactagtca agaagtcaac ggtcaaggag
    21541 ctgaaatcac gaaagaatcc gctaagcaat agtatcaaat ggggagccgc tgcccatcag
    21601 ccctgaaccc cagaggatcg cctccacctg tgtgccgggg gccagctcgg tggcgtcgga
    21661 agggaggatg acgagggcat tgatacccac aaacgagggc acagagcccg agccttgctg
    21721 tccgtgcaag gcgacgatga tctcgtcccc ctcgcgggtc gcgaccgcac gcgcgatttc
    21781 cactcttcca ggacggcgcg tgtacgcgtc ccgcagccgg gcaagcaccg gcagcggatg
    21841 gggctgcggg tctccaagca tcttccggag gcagggcgcg accaagatct cgaaggtcac
    21901 catcgcgctg atgggattgc ccggaaggcc gacgacgggc ttacccgcgt gctgcgcaaa
    21961 agccaagggc ttgcctggct tgattcggac tttccaaaac ctcggctcga tgccgacgtt
    22021 cttgaaggct tcctgaacga aatcgtaggc gccgaccgag actccgccca tggtgatgac
    22081 gacgtctgct tcgagtgcgg tgcgaagggc cgattcgatc tcgggcaggg tgtcgcgcgc
    22141 agcgggaagg gcaacgggga tagctcccag gcgatgcagc atgtccgtca atacatagag
    22201 gttcgagttc acgattgcgc ccggctcgag cggatcgccg agtgcacgga gctcatcacc
    22261 tgtcgaaaga agggcgacct tgggtcgcct gtagacctgc acgcgagcga tgttctggct
    22321 ggcgagcagt ccgatctcac caggcccgat ccgagcccca gcccgcaaga ggacggtgcc
    22381 cgctcgcaca tcgctcccgc gcgcccggac gtgcttcccc tggtgcgagg cctctcgaat
    22441 cagaacacca tcggcgtcgc tgtccgtgtc ctcttggatg acgaccgcag tggcgccggc
    22501 gggcatcggc gctccggtga agatccggta cgccgcgccg ggctccaggt acccgggaag
    22561 cgggccgccc gcacgcgact ctccctgaac cggaagacga accggcgcat ccggctctgc
    22621 cgacgccaca tcgctcgcgc gaacggcgta tccatccatc gcgctgttgt cgaaaggcgg
    22681 cgagtccagc cgcgcctcga gatcgcgcga caggtaccgg tccgagcttt cggtcagatg
    22741 cacctcctcc tcgcccagag gcgaaaacgc cggcagcatc gaagccaaag cctctgcgat
    22801 cgatcgcatg cgccgggaac cctagcacgg ctttgatgcc ggctgactag tcaggtgcga
    22861 cgcgattgcg gccggcacgc ttggcttcat aaagtcgcgc atcggccgcc cggacgaagt
    22921 catgagaccg ccaggtcggc tcgagctgcg caacaccgca ggaaatcgtg acgtcgatgt
    22981 cctcgtcctc gaagacgaaa gtctggctcg cgatcatttg gcgaagctct cctgcaatgc
    23041 gtacccctcc agcaaggtcg gtctcgggca ggatgagggc gagctcttca cccccgtatc
    23101 gcgcaatcac gtcgtccggt cggattctac tcttggcgag ttgggcaacc tctttgagga
    23161 cgtggtcacc cgcgagatgt ccgaactcgt cattgacccg cttgaaatgg tcgatgtcgc
    23221 aaatgaccaa ggtcaatggc cgttgatggc gtgtcgcgcg ggagagctca cgatccaatt
    23281 gttcgacgag ataacgcttg ttgtagatgc cggtcagtcc gtcgacgatc gtcatccgat
    23341 agatggtctc gtggtattgc gactccagat ccgcgccact cagaaacttc aagatcgtat
    23401 cgccgacttt gatctgatca ccgcggcgaa gttgacggtc gcgcacgagc tcatcgttta
    23461 cgtatgtgcc gttggtcgag cacgtgtcga tcagatggta gctgcctgcg cggatctcga
    23521 ttcgtgcatg tcggcgtgaa acgctgtcgc tcagaaggac gacatcgttc tccatgccac
    23581 ggccaattct cgccagcccg tcgacgagag agaaacgctg ccccagctga ctcgcgccag
    23641 gagcgtagat caccacgagg cagtcctggc ccgccccagt ggcgatgtgc ggcgcggaaa
    23701 agggcgcgac cctcgtcttg atttcgctct ccacaggttc cagtcagggt agaaagcacc
    23761 gaatcgagcg tcaacgtcgc cgaatgtagg tcatgtttct ctggttgatc cagacgtggg
    23821 ccaaaaccgg cccccaaaga acaccggtga gctcgaagat cagcccgaaa agaaggccca
    23881 tgacgaatgc ccaggcgatc caagctaaaa agactttcct aggccctaca tggaccagcc
    23941 cgaagatcgc cgagctcagc agcagtccca ggaccggctg catcgcccct cgaaaaaaga
    24001 gctcctcggc gatgccggag ctcaaggcga gaaccatgat ctcggacggc gacagcacgt
    24061 ggatcatctc ggcaagctct tcgtgaagag cgcgcgccca tcgagcacgt tcgacgaata
    24121 ccggagtcat cgccactacg gcgactgcga gcacgaggcc caacccgagc gaaaccagga
    24181 ggcgcaacgc ataggagcca cccatccagg gggtctcgag agcccaagcg aggcgccgat
    24241 cgcttcccca tgcccaaagc agcgcaccca cggtcagcgg ggcgtagact agcagagcga
    24301 gccgcgtgat ccgggtaggc gatggcaaga gcggttcgca tgtatcaaag tgaccgccta
    24361 accgcaatgc cagccagacg cagaattgac acgagagcgc agggcagagt agtagccagg
    24421 atcatacgcg catgagcagc ccgcctgccg atgacgtcgc gaagatcctc gtggtcgacg
    24481 acgaggaagt catccgtgag attctcgcgg acttccttac tatggagggc ttctccgtgc
    24541 gtaccgccaa agacgggggc gatgcgctgg tcgagctctc gcgggatcgc ttcgacctgg
    24601 tcttgagcga cttgaagatg ccgaacatgg gcggcatcga gctcctcggc gccatccgga
    24661 agcacactcc tcacgtcgtg acgatcatca tgaccggatt cggcaccgtc gaaaccgcga
    24721 tcgaggcgat gaagcagggc gcatacgact acgttctcaa gcccttcaaa gtggaagagg
    24781 tcgtgcatac gatccgtcgc gccctcgaaa cgcaacggct cgaagcggag aactttcgcc
    24841 tcaaggaggc gctctccctc tacaaagtga gcgaggcgat cgcggccagc ctctcgctgg
    24901 agcaggtcgt ccacactctc agccgtgccg cgatcgacga agtcgactcc gacctggtga
    24961 ccgtactgct cagcgacgcg gcgggaagct tctatcgacg tagcaccgag ctcaacccga
    25021 acttccgctt gaggcgcgaa cccgacgagt tgagcatccc ggcgctgacg gagcatttcg
    25081 ttcatgcgcc ggccctgctc gagcatacgc agcgatgttg gcgttacttc gagtccgtcg
    25141 cagaagaccc cgcgccgcag tctctggcgg tcgcgcgtct gactgcccgc ggggaaacga
    25201 tcggctttct ctgcgcactc agctataccc agggcaagcg cttcgacgag ggccagcgaa
    25261 agcttctgca tgcgacggcc gaccgcgcgt cagcggcaat cgaaaacgcc cgcctctacg
    25321 aagaccttca ggcaaccttc aaacaaacca tcaggggcct ggcgagcgcg atcgacaaga
    25381 tggatcgata tacggcgggg cactccgcga gggttgcggc ttatgcgcaa atcctcgcca
    25441 tcaagctggg cctcggcgac gccgagattg aagtcgttcg ccaatctgcc ttgatgcacg
    25501 acatcgggaa gatcggttgc gtgatgaatc tgaacaagcc cggcaagcta tcccaggaag
    25561 aatacgagat cttcaaggag caccctgagc atggccggga catactgcag ccgatcgagt
    25621 ttctccatcc gctcattccc ggggtgcatc tgcatcacga gcgatgggat gggctcgggt
    25681 atccgctagg gctcaaggga aacgacgttc ccctcgtcgc acggatcatc gcggtcgccg
    25741 atacctacga cgccatgacg agcgaccgcg cctatcgaaa agcgatgagc cacgacatcg
    25801 cgatctccga gatcgatcga tgcggcggaa gccagttcga cccggagctc gcggacggct
    25861 tccttcgctc gatcgaggat ttccgggtcg aaggacgatc gctcgaagag tttcccgacg
    25921 tgcgcgagac ccgatccggc ctcatggcgc cacggaggtg acggcgccgc tcggcggctc
    25981 tggcatcgca aacgcagcat tccagtgccg cgagcggggc gttgcagacc agcggcgaac
    26041 gatcggaggc tggtccccaa ctttgaaaat cgtaacgagc gttagactgc cgagacgcat
    26101 ctcccggatg ggcaaatgcg tcaccgatac gagcgtcagc gtttgattgg gtcctatggc
    26161 cgggtcagcg tagtccaagt cgcccaggcc ttcggagacc aaacgccgaa tcgtattctc
    26221 gtttcgaaac gagcgagtcg tggtcacaag gcgcccgtgc tcgctgtcgt ac
//
LOCUS       DASRZF010000003         2207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2409988, whole genome shotgun sequence.
ACCESSION   DASRZF010000003 DASRZF010000000
VERSION     DASRZF010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2409988"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VFG22_00130"
     CDS             <1..501
                     /locus_tag="VFG22_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414668.1"
                     /translation="LVAIPGQEGLHPAAAESIRELSGIDLFSEFSDPKPGEAWLVRLD
                     SPPRAERFKLGRRFVQHVRHWHKYAEARLPPARVFRFRSFSGPTGAQADNLSAFRREL
                     LLCDSKVLQHHAANHDFSHWLEHAISDETLARVAHELEKHGAVDGSFEDLRRGLVEAI
                     EDRYLG"
     gene            512..1672
                     /locus_tag="VFG22_00135"
     CDS             512..1672
                     /locus_tag="VFG22_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414669.1"
                     /translation="MSMEPLEEAAEIMMRFAERTGLGADRSPRRYLWTDAFAVCNFIG
                     LARATGDDRYTELALRSVDQVHEVLGSYRADDRRVGRLGGLSDEEGEVHPTRGGLRIG
                     KALPERAASEPFDERLEWERDGQYFHYLTKWMHALDQVARWTRQAQFNTWARELAEVA
                     HAAFSYSSQNGRRMVWKMSTDLQRPLVASMGHHDPLDGFITALQLQTTRSALGTAASE
                     PTLEREAADFAKMIQDQDWATADPLGLGGLLTDASRVAQLMQLGAFAGGELLDDLLSS
                     ALPGLSYWAGQNDSGLPASRRLAFRELGLAIGLSGIELIDEAVRREHLALSAKTNEGL
                     EALTPYAHLGSEIRSFWLDPEHREARTWSEHRDINEVMLATSLVPAGFLRIS"
     gene            1739..>2207
                     /locus_tag="VFG22_00140"
     CDS             1739..>2207
                     /locus_tag="VFG22_00140"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET6414670.1"
                     /translation="MFSERGAFDLGPNVIARAVEATPPRWDLTHSNPTRAGIAFGDEA
                     MRAMQTASLDLYDPQPRGLASARQALGEHLDWNPDHLVLTATTSEAYSSLIKLFCDPG
                     DQVLIPEPSYPLLPMLARLDGVVALPYSFRYDGQWHIDRTAFAEGVGPEVKLIL"
BASE COUNT          387 a          665 c          732 g          423 t
ORIGIN      
        1 ttggttgcca tccctgggca agaagggttg catccggcag ccgcagaaag tatccgtgag
       61 ctatctggca tcgacctgtt cagcgagttc tcagacccaa aaccgggcga ggcgtggctc
      121 gtgcgactcg acagtcctcc acgcgcagag cgcttcaaac tgggccggcg cttcgtgcaa
      181 cacgtgcgcc actggcacaa atatgccgag gctcgccttc ctcccgccag ggtgttccgc
      241 tttcgctcgt tctccgggcc cacgggcgct caagccgaca atctttctgc atttcgccgg
      301 gagctactct tgtgcgattc gaaggttctg cagcaccacg ccgccaacca cgatttctcg
      361 cactggctgg agcatgcaat aagcgacgaa acgctggcac gcgtcgcgca tgagctcgag
      421 aaacacggcg cggtggacgg gtcgttcgaa gaccttcgtc gtgggctggt cgaggcgatc
      481 gaggatcgct atctgggata gggtctgcgc gatgtcgatg gaaccgctcg aagaggctgc
      541 tgagatcatg atgcgttttg cggagcgcac gggtctcggc gcggatcggt ctccgcgtcg
      601 ctacctatgg acggacgcct ttgcggtctg caacttcatc ggtctggcgc gagctactgg
      661 cgacgaccgg tacacggagc ttgcgctgcg ttcggtcgat caggttcacg aggtgctcgg
      721 ctcgtatcgg gccgatgatc ggcgcgtcgg gcggctcgga ggactcagcg atgaagaagg
      781 tgaggttcac ccgacgcggg gtggcctccg aatcggcaag gccctccccg agcgggcggc
      841 ctccgagccg ttcgacgagc gactggagtg ggaacgggac gggcagtact tccactacct
      901 gaccaagtgg atgcacgcgc tcgatcaggt ggcgaggtgg acccggcagg cgcagttcaa
      961 cacttgggcg cgcgagctcg cggaggtggc gcacgcagcg tttagctact cgagtcagaa
     1021 cggccgacgg atggtctgga agatgagcac cgaccttcag cgcccgttgg tcgcctcgat
     1081 ggggcaccac gatccgctgg atggattcat tacagcgctt cagcttcaga cgaccaggtc
     1141 ggcccttggg accgcggcga gtgaacctac gttggaaagg gaggcggccg acttcgcgaa
     1201 gatgatccaa gaccaggact gggccacggc ggacccattg gggttgggag ggttgctgac
     1261 ggatgcgtct cgcgtcgcac agctcatgca gctcggggcc tttgccggag gtgagctact
     1321 cgacgatcta ctttcctccg ccctgccggg cctttcgtac tgggctgggc agaacgactc
     1381 cggactccca gcgtcgcggc gcttggcctt tcgtgagctt gggctcgcga tcgggctttc
     1441 cgggatcgag ctcatcgacg aggcagttag gcgggagcac ttggcactga gcgcgaaaac
     1501 aaacgaaggg ctcgaagcgc tgacgcctta cgcccacctc ggatcggaga ttcggtcgtt
     1561 ctggctcgat ccggagcatc gcgaagcccg aacctggtcg gagcaccgtg acatcaacga
     1621 agtgatgctg gcgaccagcc tcgttccggc cgggtttctg cggatttcat gaccggacga
     1681 agacgggacg gtcgaaggcg acctggcgct tgggtctggc gacggggata ctcgcgcgat
     1741 gttttcggaa aggggcgcgt tcgacctcgg ccccaatgtc atcgctcggg cagtggaggc
     1801 gacgccgccc agatgggatc tgacccattc gaatccgact cgcgctggca tcgcttttgg
     1861 agacgaggcg atgcgggcca tgcaaaccgc ctcgctcgat ctctacgacc cgcagccgcg
     1921 ggggcttgcg tcggctcgcc aagcgctggg tgagcatctc gactggaatc ccgatcacct
     1981 ggtgctcacc gctacgacga gcgaagcgta ttcgtctctg atcaaactct tctgcgaccc
     2041 gggggaccag gttctaattc cggagccgag ctatccgctc cttccaatgc tcgcacgtct
     2101 cgacggcgtc gtcgctttac cgtattcgtt tcgctacgac gggcagtggc acatcgatcg
     2161 cacggctttc gccgagggtg tgggtcctga ggtcaagctg attctag
//
LOCUS       DASRZF010000004         7350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1722555, whole genome shotgun sequence.
ACCESSION   DASRZF010000004 DASRZF010000000
VERSION     DASRZF010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 7350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7350
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1722555"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="VFG22_00145"
     CDS             <1..422
                     /locus_tag="VFG22_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414671.1"
                     /translation="SGLWMVGCGDDGACPGGQVSCDDVCIDAIEPTLAGADGIQERVF
                     QGSCAFTNCHGAEGGVQADLELSSASISADNLIDVQSTEVDGLRVAPGNTGASYLVDK
                     LLGQNLAPGTAQMPNVGTALCDAKIQAVEAWIEAGAN"
     gene            444..1400
                     /locus_tag="VFG22_00150"
     CDS             444..1400
                     /locus_tag="VFG22_00150"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6414672.1"
                     /translation="MEYRRLGSSGLKLSVISFGSWVTFHNQLGHETALRCMKAAWEAG
                     VNFFDNAEVYAGGKSEIIMGHVARELGWPRESFVISTKLFWGLTAGPNTKNTLNRKYL
                     MHGIDGSLERLGLEHVDLLFCHRADPETSVEEVVWAMSDIIGAGKAHYWGTSEWPAEA
                     IEEAYEIAERYRLRKPVMEQPQYSLLSRDRVEREYAPLYQKHQMGTTVWSPLASGALT
                     GKYLDGVPADSRAALPGYEWLREIVTSPDNHAQIRRAIEVAEELGCTPAQLAIAWCAK
                     NPRVSTVITGASRVEQVHENLGALDVIPRLTDEVMARLEGRA"
     gene            complement(1397..3388)
                     /locus_tag="VFG22_00155"
     CDS             complement(1397..3388)
                     /locus_tag="VFG22_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414673.1"
                     /translation="MRVNPLLLLLVLLVTVSCSSGGASSTGYQPTTDTSDLCTTPLAS
                     DEPLCNTSFGDSVWSGAHRASYAQGSSPLAGPSDSAGTSSAHLILAGAGVPVITSFTK
                     PYADGGRAIWSTATGADAAVVKIDHETFGIIDWYVPAEREDSPPTFALGLSGAYTAID
                     SQNRFIVGRTRFVGFYGDSVEGDRSSPIELKKRIFIPDEAFCNGSDVIAGMSLTYDGH
                     LALATELGNLFVISLDADPENLGDVPVLSANDDCVSTDEDDLEIVSNSIAVDENGGVY
                     LLTSAAMYRFDWNGSVLQRIWRAEYESADEVPSPIRLGPGSGSTPSLMGTRADDDRFV
                     VITDGQALMNLVLFWRDEIPGDWQPIAPGRDPRIACEVPVDFGTGAIEAISEQSVAVR
                     GYSAVIVNDLLTNPTINPPTMLALGALAQNLVSALEGGIPEKAPFGLERIDWDPETRT
                     CSTVWANATVSVPNGIPSISGASGLVYGAGQRDGQWGLEGLDFDTGESRVWVEAGSGV
                     CAGESSNLASILPGVSEVLEQVPDSCENSVYAATTVGPDGTVYQGTLNGMTRYVPDAV
                     EASPSASQAEAGVEQALDLLSRSQSDAPSNVSERDFLRRALVQLMATQSVAREAGLGE
                     LSVAVAGAMQALDAAVAALDAGEPYDAYVETAREALVAF"
     gene            3445..3834
                     /locus_tag="VFG22_00160"
     CDS             3445..3834
                     /locus_tag="VFG22_00160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HET6414674.1"
                     /translation="MTAFDALSKARYVNLETFRKNGTGVQTPVWGAPNGDEIVIFTNG
                     DSYKVKRLRRNPRVRIAECGVRGALKGPWHDGTGRLVSDEAGKQSALRALRKKYGWQM
                     LLADWGGRIRGSKKNWQIIAVRLGADS"
     gene            complement(3838..4572)
                     /locus_tag="VFG22_00165"
     CDS             complement(3838..4572)
                     /locus_tag="VFG22_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414675.1"
                     /translation="MKKLRLGFLTVIVMGCLPASFAAAQWDASPEEGPYTTAPTPPPQ
                     QMVSTTPQNFVEQPQEKKGLDVSFTWSLGIGVPIVLDVPHDIVRPGANLFFFGGADFG
                     WFIIGGDVGLQWTPINLDNAPPVNGVTFSGRHPLTRIYLTLPEVRFQVPDLKVVLPYI
                     SGAFDMNFWNFAEQAIGCGYWYCSAYNVYRFTPGFTGKAGLGFEVKNGVYIDTGFQFS
                     FTGKGNFFEDSGWWLAPYVGVLVRRR"
     gene            4725..6455
                     /locus_tag="VFG22_00170"
     CDS             4725..6455
                     /locus_tag="VFG22_00170"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HET6414676.1"
                     /translation="MTSAKPGRALEPTAIFVFTLAIASLLPLVGLGQEDQAKQEKTVT
                     EQVAELIADLQTREADLEEMRLKIAKNPDNAAAKILEKELSERREKYRRDSSKLVKLV
                     LNGEDAGADVATGRAAVTEILEKDAQSMLEKSRELDQKIVELMNIVDGGTGEEAQIAR
                     AKMIDSIPISDRLISYIDTNLHEQEQLGLDVQAESTNLLKRLETRADIVSGLLRNTKS
                     EVDAITRNPGAGQDAEAQKQLAELKRQRDALAESQRSTVRILSERGVDTAMLRQEIIT
                     ATGKLSEDILDKDVAAGLIEQWFANTAEWFRSNGASLLFQVLVFFLILFGFWIVSRIV
                     RGAVRRGVERSKLNISSLAKDFFIKMTSRMVMLLGVIIAIAQIGVEVAPLLAGLGIAG
                     FVVGFALQDTLSNFASGLMILVYRPFDVGDAIEAGGVTGKVDQMNLVATMVLTFDNQL
                     LIVPNKQIWGGVIRNITHQATRRIDLTFGIGYSDDIPKAEKVLAEIVAAHEKVLKDPE
                     PVIRLHELGDSSVNFVVRPWSKTSDYWDVYWDVTREVKRRFDEEGISIPFPQRDVHIY
                     RENGDQPKEA"
     gene            complement(6452..>7350)
                     /locus_tag="VFG22_00175"
     CDS             complement(6452..>7350)
                     /locus_tag="VFG22_00175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6414677.1"
                     /translation="FHAADARRIRARFPVPVEAPLALRPGCFKEAGMKTAVVIGAGKG
                     LGREIAKGLAKKGFAVLVTDIDGEAARLTAETIGAGAWSIQQDVRDPASHQAIARAAN
                     ERGVLGLWVNNAGVLHTGNVWDHSDEVVRQMIDVNLLGMIWGSRAAVDAMRAQGGHLI
                     NIASLSSITPVPGLAIYGATKQAVLGFTISLAGDLERAALPIKVSAVCPDGIDTDMVR
                     NVRGSKQAGIVFAAPKLLTVEEVTNVVLELVDKPKLAVSVPRSRAALAHALRPFPALG
                     LKALQPFIRYGERRRRKINDEA"
BASE COUNT         1502 a         2234 c         2295 g         1319 t
ORIGIN      
        1 gctcaggctt gtggatggtg ggttgtggcg acgatggagc ttgcccgggc gggcaagtga
       61 gctgcgacga cgtgtgcatc gacgcgatcg agccgacact tgccggcgcc gacggcattc
      121 aggaacgcgt ctttcagggc agctgcgcgt tcaccaactg ccacggagcc gaaggtggtg
      181 tgcaagcgga cctcgagctc agctcggctt ccatatcggc tgacaacctg atcgatgtgc
      241 agtccacgga agtcgacggg ctcagggtcg ctcctgggaa cacgggcgcc tcctacctgg
      301 tcgataaact gctcggccaa aacctcgctc cagggacagc gcaaatgccc aacgtcggca
      361 cagccctatg cgacgcgaag atccaagccg tcgaagcatg gattgaggcc ggagcgaact
      421 agcaccgggc caagcagatg gagatggaat accgacgatt gggtagctcg ggcctcaagc
      481 tcagcgtgat ttcgttcggg tcctgggtga cgtttcacaa tcagctcggc cacgaaaccg
      541 ccttgcggtg catgaaagcc gcctgggagg cgggggtgaa tttcttcgac aacgccgagg
      601 tctacgcggg gggaaagtcg gagatcatca tgggtcacgt cgcccgcgag ctcggatggc
      661 ctcgagagtc gttcgtgatc tcgaccaagc tcttttgggg cttgacggcc gggcccaaca
      721 cgaagaacac cctcaatcgc aaatacctga tgcacgggat cgatggctcg ctcgagcgtc
      781 tgggcctcga gcacgtcgat ctcttgtttt gccaccgcgc agaccctgag acgagcgtcg
      841 aagaggtcgt ctgggcgatg agcgacatca tcggcgccgg caaggcgcac tactggggca
      901 cctcggaatg gcctgcggag gcgatcgagg aggcctacga gatcgccgag cggtatcgac
      961 ttcgaaagcc tgtgatggag cagccgcagt acagcctgct ctcgcgcgac cgggtcgaac
     1021 gagaatacgc gccgctctac cagaagcacc aaatgggaac gacggtttgg agcccgctcg
     1081 cctcgggtgc gctgacaggc aagtacctcg atggcgtgcc tgccgatagc cgcgccgcat
     1141 tgccaggcta cgagtggttg cgcgagatcg tcacgtcacc ggacaaccac gcccagattc
     1201 gacgggcgat cgaagttgcg gaggagctcg gctgcacacc cgcgcaactc gccatcgcct
     1261 ggtgcgccaa gaatcctcgt gtgtcgacgg tcattaccgg cgcgagccgc gttgagcagg
     1321 tacatgaaaa cctaggtgcc ctggacgtga tcccgcggct caccgacgag gtgatggcgc
     1381 gacttgaagg ccgcgcctag aaggcaacca aggcttcgcg cgccgtctcg acgtaggcgt
     1441 cgtaaggctc acctgcatcg agggctgcta cggcagcgtc gagggcttgc atggccccgg
     1501 ccactgccac gctcagctct ccaagccctg cttcccgtgc aaccgactgc gtggccatga
     1561 gctgcacgag ggcccgcctc aggaaatccc gctcgctcac gttcgaaggg gcgtccgatt
     1621 gcgagcgcga caggagatcg agagcctgct caacgcccgc ctccgcttgt gaggccgacg
     1681 ggctggcctc gaccgcgtcc ggaacgtagc gtgtcatccc gttcaatgtg ccttggtaca
     1741 cggtcccgtc cggaccgacc gtcgtcgcgg cgtagaccga gttctcgcag gaatctggga
     1801 cctgttcgag tacctccgac acgcccggaa gaatcgacgc gaggttcgag gattcgccgg
     1861 cgcaaacccc agagccagcc tcaacccaaa ctcgcgactc gcccgtgtcg aagtccagtc
     1921 cttcgagacc ccattgaccg tcgcgctgac cggcgccgta gaccaaaccg gaggctccgc
     1981 taatgctcgg gatgccattc ggaacactga cggtcgcgtt ggcccagacc gtggagcaag
     2041 ttcgcgtttc cggatcccag tcgatgcgtt cgagaccaaa cggcgctttc tccggaatac
     2101 cgccttcgag cgcggagacg aggttctgcg cgagtgcacc gagcgccaac atcgtgggcg
     2161 gattgatcgt cgggttagtg agaaggtcgt tgacgatgac cgccgagtag ccccggacgg
     2221 cgacggactg ttcggagatc gcctcgatcg ctccggtgcc gaagtcgacc ggaacctcgc
     2281 acgcgatacg aggatctctg cccggcgcga tcggctgcca gtcgccgggg atctcgtcgc
     2341 gccagaagag aaccaggttc atcaaggctt ggccgtccgt gatcacgacg aagcgatcgt
     2401 cgtcggcgcg cgtgcccatg aggcttggtg tcgatccgga accgggtcct aaacggatag
     2461 ggctcgggac ttcgtctgca gattcgtact ccgcacgcca gattcgctgc aacacgctgc
     2521 cgttccagtc gaaccggtac atcgctgcgc tggtgagcag gtagacgcca ccgttttcgt
     2581 ctacggcgat gctattggag acgatctcga gatcgtcttc atccgtgctg acgcagtcgt
     2641 cgttcgcgga cagaaccggc acatcgccta ggttttctgg atcggcatcc agcgaaatga
     2701 cgaagaggtt cccgagctcg gtcgcaagcg ccaaatgacc gtcgtaggtg agtgacatcc
     2761 ctgcaatgac gtcgctccca ttgcagaacg cttcgtcggg aatgaagatt cgcttcttga
     2821 gctcgatcgg cgatgagcga tcgccctcca cgctgtcgcc atagaaaccg acaaatcgag
     2881 tgcgacccac gatgaatcgg ttctgcgaat cgatcgccgt ataggcgccg ctcaacccga
     2941 gcgcgaatgt aggagggctg tcctcgcgct cggctggaac gtaccagtcg atgatcccga
     3001 aggtctcgtg gtcgattttg acgaccgctg catcagcccc ggttgcggtc gaccaaatcg
     3061 cgcgaccgcc gtctgcatac ggcttggtga atgaagtgat caccggaacg ccagcgcctg
     3121 cgagtatgag atgcgcgctg ctcgttcccg cactgtcgct cggcccagca agcggcgaag
     3181 acccttgcgc gtagctcgcc cggtgcgcac ccgaccagac cgaatctccg aacgaggtgt
     3241 tgcagagggg ttcgtcgctc gcgagcggtg tcgtgcagag atccgacgta tcggtcgtgg
     3301 gctggtatcc cgtggaactg gcgccgcccg agctgcagct gaccgtcacg agcagaacca
     3361 agagcaaaag caagggattg acgcgcacta tagatgcctt ctagcaggag atgcgccgag
     3421 gccaaacggc gttatgatcg ctccatgacc gccttcgacg ctctatccaa agcccgctat
     3481 gtgaatctcg aaacgttccg aaagaacggg accggtgttc agactccggt ctggggcgcc
     3541 cccaacgggg acgaaatcgt cattttcacc aatggcgact catacaaggt caagcgattg
     3601 cggcgcaatc ccagagttcg aatcgcggag tgcggggtcc gcggcgcgct caaggggccc
     3661 tggcatgacg ggaccggtcg actggtgagc gacgaggccg gtaagcagtc cgcgctccgg
     3721 gcactgcgca aaaagtacgg ttggcagatg ctgctcgccg attggggcgg tcgaatccga
     3781 ggttcgaaga agaactggca gatcatcgcc gtgcgcttgg gcgccgattc gtagccgcta
     3841 tctacgtcga actagaacgc cgacgtaagg tgctagccac cagccgctgt cttcgaagaa
     3901 gttacccttg ccggtaaacg agaactggaa gcccgtgtcg atgtagacgc cgttcttgac
     3961 ctcgaaaccg agaccggcct tgccagtgaa gcctggcgtg aatcgataca cgttgtaggc
     4021 gctgcaatac caatagccgc aaccgatcgc ttgctcggcg aagttccaga agttcatgtc
     4081 gaacgcaccg ctgatgtacg ggaggacgac tttgagatct gggacctgga agcggacctc
     4141 cggaagcgtg aggtagatgc gcgtcagtgg atgccgaccg gaaaacgtga cgccgttgac
     4201 tgggggggcg ttgtccaggt tgatgggagt ccactgaaga ccgacgtcgc cgccgatgat
     4261 gaaccatccg aaatcggcgc ctccgaagaa gaaaaggttc gcgcccggtc gcactatgtc
     4321 gtgaggcacg tccagaacga tcggcacgcc gataccgagg gaccaggtga agctcacatc
     4381 gaggcccttc ttttcttggg gctgctcaac gaagttttgg ggcgtcgtgg acaccatctg
     4441 ctgaggcgga ggagtcgggg ccgtggtgta gggcccctct tccggagacg catcccattg
     4501 agcagccgca aagctcgccg gcaggcaacc catgacgatg actgtcagga atccaaggcg
     4561 cagtttcttc atcggaacct cccagaatgc gctcagctat acccgagcag aactttcgat
     4621 tcaatgtttc tggcgacttg agccgtgcca agacttcgac tagcactccg gctctttcca
     4681 gggtcggtcc cacggccggg caaccacttt taggggtact caacatgacc agcgcgaaac
     4741 caggtcgggc gctcgagccg accgcaatct tcgtattcac cctcgccatc gcctctttgc
     4801 ttccactcgt ggggctcggg caggaagacc aggccaagca agaaaagacc gtgacggagc
     4861 aagtcgcgga gctcattgcc gacctacaga cacgggaggc ggacctcgaa gagatgcgtc
     4921 tcaagatcgc gaagaacccc gacaacgcgg ccgccaagat tctggagaag gagctgagcg
     4981 agcggcggga gaagtatcgc cgcgactcgt caaagcttgt gaagctcgtc ctgaacggcg
     5041 aggacgcagg ggccgacgtg gccactggca gggccgctgt gacggaaatc ctcgagaaag
     5101 acgcgcagtc gatgctcgag aaatcccgcg agctcgacca gaagatcgtc gagctaatga
     5161 acatcgtcga cggtggaacc ggcgaggaag cgcaaatcgc gcgtgcgaag atgatcgact
     5221 cgatcccgat atccgaccgc ctgatcagct acatcgatac gaaccttcac gagcaggaac
     5281 agctcggtct ggacgtccaa gcagagagca ccaacctgct caagcgcctc gaaacccgcg
     5341 cggacatcgt ctcgggcttg ttgcggaaca ccaagtccga agtcgatgcc atcacccgga
     5401 accccggtgc aggccaggat gccgaggctc aaaagcagct cgccgagctc aagcgccagc
     5461 gagacgccct cgccgaaagc caacgtagca ccgtccgcat cctgagcgaa cgcggtgtcg
     5521 acactgcgat gctccggcag gaaatcatca ctgcaacggg caagctctct gaggacatcc
     5581 tcgacaagga cgtcgcggcc gggctgatcg agcaatggtt tgcgaacacc gctgaatggt
     5641 ttcggagcaa cggcgcgtcg ctgctgttcc aggtgctggt tttctttctg atcctattcg
     5701 gtttctggat cgtgtcgcgg atcgtgagag gcgcggttcg tcgcggagtc gagcgctcga
     5761 agctcaacat ctcgagcctc gctaaggact ttttcatcaa gatgacgagc cggatggtca
     5821 tgctcctcgg cgtgatcatc gcgatcgccc aaatcggcgt cgaggttgca cccttgctcg
     5881 ccggcctcgg aatcgcgggc ttcgtcgtcg gcttcgcgct gcaggatact ctttcgaact
     5941 tcgcatccgg actgatgatc ctcgtctatc ggccattcga tgtgggcgac gcgatcgaag
     6001 caggcggtgt cacgggcaaa gtcgaccaga tgaacctcgt ggcgacgatg gtgctcacct
     6061 tcgacaacca actcctgatc gtgcccaaca aacagatctg gggtggagtc attcgcaaca
     6121 tcacccatca ggcgacccgg cgcatcgact tgacgttcgg cattgggtac tccgacgaca
     6181 ttcccaaggc ggagaaggtg ctagccgaaa tcgtcgctgc gcacgagaag gttctgaaag
     6241 acccagagcc ggtcattcgt ctgcacgagc tcggagactc ttccgtgaac ttcgtggtgc
     6301 gtccttggag caagacctcc gactactggg acgtgtactg ggacgtcact cgcgaagtga
     6361 agcgccgctt cgacgaagaa gggatttcaa tccccttccc gcaacgcgac gtgcacatct
     6421 atcgagaaaa tggagaccag ccgaaggagg cctaggcctc gtcgttgatc ttgcggcgcc
     6481 tgcgctcgcc gtatcgaatg aacggctgaa gcgccttgag gcccaacgcg ggaaagggcc
     6541 tgagtgcgtg cgccaaggcg gcacgcgaac gcggcacgct caccgcgagc tttggtttgt
     6601 ccacgagctc gagcacgacg ttcgtgactt cctcgaccgt gagaagcttg ggagcggcaa
     6661 acacgatccc cgcttgcttc gagccgcgga cgtttcggac catgtcggta tcgattccat
     6721 cggggcagac cgcgctgact ttgatgggaa gcgcggcacg ctcgaggtcg ccggccagcg
     6781 agatcgtgaa cccgagaacc gcctgtttgg ttgcaccgta gatcgccaat ccaggaacgg
     6841 gcgtaatcga cgatagggag gcgatgttga tcaagtggcc accctgggcg cgcatggcgt
     6901 cgaccgcagc gcgtgaaccc cagatcattc cgagcaggtt gacatcgatc atctggcgaa
     6961 cgacttcatc gctgtggtcc catacgtttc cggtgtgaag cacgccagcg ttgttgaccc
     7021 agaggccgag gacacctcgc tcgttcgccg cgcgtgcgat cgcctgatgg gaggcagggt
     7081 ctctcacgtc ctgttgtatg gaccaagctc cagcgccgat ggtctcggcc gtcaggcggg
     7141 cggcctcccc gtcgatgtcc gtcacgagga cggcgaatcc cttcttggcg agacccttgg
     7201 cgatttcccg cccgagaccc ttgccggctc ctatcacgac cgctgttttc atgcctgcct
     7261 ctttgaagca tcccgggcga agagcaagag gtgcctcgac cggcactgga aagcgggcgc
     7321 gaatccgtct agcatcggcc gcatgaaata
//
LOCUS       DASRZF010000005        25425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2753673, whole genome shotgun sequence.
ACCESSION   DASRZF010000005 DASRZF010000000
VERSION     DASRZF010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 25425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25425
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2753673"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /gene="argE"
                     /locus_tag="VFG22_00180"
     CDS             <1..965
                     /gene="argE"
                     /locus_tag="VFG22_00180"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HET6414678.1"
                     /translation="KLGEGEDGLVLSGHTDTVPYDADRWSDDPFVLTEHDGKLHGLGT
                     ADMKGFFAAALHAIARFEPGALRRPITILGTADEESTMNGARALAKKGERLGRYAIIG
                     EPTGLAPIRKHKGVFYVQVTVSGKSGHASNPALGVNAIEGLQLVLAALQSWRDTIGAR
                     YHDEDFEVPGPTLNFGRVSGGDSPNRICAESKLTLDVRLLPGMQRSEVTAELRDVVAN
                     ALDGGPWGFDVKGVGGYADAFGGPSDAQFCTAVETLCGCSARTALFGTEAPFLTELGL
                     QTLVLGAGDIAVAHQPDEYVTMAAIERATDVYAKLIKRFCIEPA"
     gene            962..2281
                     /gene="argA"
                     /locus_tag="VFG22_00185"
     CDS             962..2281
                     /gene="argA"
                     /locus_tag="VFG22_00185"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF003641.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HET6414679.1"
                     /translation="MSPQVDPFVQHFRASAPYIHAHRGRTFVIMFGGEAVASKELRTL
                     LYDVALLHSLGVRIVLVAGARPQIDEALARRGEKPMYEGGLRVTDEVALECVKEAVGA
                     TRVELESLLSMGLPNSPMAGARLRVAAGNFVMAQPVGVVDGVDYQYTGKPRRIDAEAI
                     GQRLDDGAIVVLNPIGYSSTGEAFNISSHDVAAATAIALRADKLIGLLEGPGVLDAEG
                     LAQSQLSPNEAERLLQSGADLGHDVERHLGAAITACRGGVPRAHLVSRQSDGSLLREL
                     FTRDGIGTLVTSEPFEGVRHAAQSDIGGILALIEPLEEQGILVRRSQEMLENDIDRFT
                     VIERDGMVVACAALYGYPDDSIAELACLVVHPDYRTSGRGDQLLQYLERQCAAQGLKR
                     LFVLTTQTSHWFRERGFDNCELGELPEARRSLYDLGRRSKILLKPLD"
     gene            2397..4781
                     /locus_tag="VFG22_00190"
     CDS             2397..4781
                     /locus_tag="VFG22_00190"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HET6414680.1"
                     /translation="MKQAELNPRPEADAPPADAEMDEIVAQEEKVLARVHRTVAARRP
                     SHRGQLIDYDAELIALRDQIREARLEDIPPLVEEMERLQQVAQRRAKVTEGSVDRGSP
                     YFGRMILEEEGRKREILIGRSTYLDPKTGVRIVDWRDAPVSRVYYRYEEGDDYDEIFG
                     DREVEGEVLVRRNLSITTGKLRRIGAPQGTFRRSRDDIWRRVAVLETKLSGGQGAAMR
                     PESHHRPGELGIGEDDIREDKHLSEITALIDPVQFELISRPTSGLVVIQGGAGSGKTT
                     IGLHRLAFLAFQDPDRFRSELMLVIVFNDALVRYISRVLPALGVEDVPVQTFQRWAEQ
                     QRRRHFPKLTTRYVEDTPTHVVRMKKHPAMLRAIDQYVAELSEKAEAELLQATDQSDD
                     SARVSRIWRGTAAQPLGVRLEVLSQWLQDRTGDAQQLSAGTRHALGRICNRWTSRARD
                     VVTAWADLLTDLQRLTGVFNRWAPDALTDKEIEWAHSWCSRHSTGVLAAYEESIEDDD
                     SQTRSTGVDGLDEEEPAELDVEDDALLLRLFQTLRGPLSGRRGSLRYEHIFVDEAQDM
                     SPVELAVVLDTATEQQSVTLAGDIAQKLHLDNGFSDWRHVLEELKLDHIEIEPLKLSY
                     RSTHEILDFATDVLGPLRNEVSGVATRYGAPVELFQFGGSGEAVGFLSEALRALVSSE
                     PMSSVAVIARYPEQADLYYKGLRHGEVPNLRRIAEQDFPFRPGVDVTDVRQVKGLEFD
                     YVVIVECNRGEFPADDESRHLLHIAATRAAHQLWVFSTGRPSLLLPESLVRPQD"
     gene            4990..8403
                     /locus_tag="VFG22_00195"
     CDS             4990..8403
                     /locus_tag="VFG22_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HET6414681.1"
                     /translation="MADTALQDTLENEASPELNPDALADDNAPTWREPTTEVAKKLAS
                     MSDRAIQRVTGGNAFLRGRLYARRKAVESLKEDGHSARGEISVRTADEPYRTAISYKE
                     ESDSWESSCTCPGWRGPTGHCKHVAAVMVALRDEVRPPRSKGQPQQTKKKKSEPLHVP
                     GTVSVGGKRRRSRRRRRGATGGEGGGLEILSPREMQAKRGEARGPMDAWLPDEALDKP
                     LDFEYRMTVRPASITVTPVLAGSRSAVPIADALGAFNMVSIDERPLFRALARNSNRGQ
                     TTTAELRGEDAAEVLEMLSDRRVLLEPASMELRFANEALKPRIELDPANGDSLRVRVV
                     FYLESNKRRFALSSGAWFEGTPGWQIDTTEGVARPVSELVTPAWLQRLYRSPAMVQSH
                     DDLPALLGEYIPRISASLGADLPDLSSVADLVDQKPRFELKANGDIIDARVRLYVRYE
                     NQEFEVPPDDFPSPLAFLPPKRGESRPRVVRRDVGAEMAGIQALLNQNLEVSESGDEL
                     VATGDDAIAFWTNGIGELPEEWDRFIPDDLVDVTIRDESVSPQMRVSSGVDWLNLDMK
                     FESGGAVVREEELRACLEHGRRLVRLEDGTFAPVDPEKVGDVLTRMAEIYATAGKSEK
                     LPLSQAGRVQDLLKMVENAKVSATAKTLFKKIEHIDEIPSIAKPRTLKADLRPYQKDG
                     FSWLVFLHELNSGGILADDMGLGKTIQTIALLLWAKSKSKRKLNLVIAPTSVVPNWER
                     EISKFAPGLKTVVWQGPNRNQQASDLEKADVMITSYALLRRDEELLQALDLGYVILDE
                     AQHIKNPMSQTARSAKKIRSERRLALTGTPIENRLSELWSIFDFVSPGLLGPLKAFEE
                     RIARPIDRGDMETAQRLRATIKPFVMRRLKRDVAADLPDKIEQEMIVPLAEEQAKLYK
                     QVLGQVRKSVLSEVEKKGVSKAQIQILAALTRLRQVACDPRLMKLPDTDFSADDSGKL
                     GALLEIIDEAVDGDHRVLVFSQFVEMLTHIRTALDSAGVKYEYLDGSTKNRLEKVDHF
                     NDDSSVPVFLISLKAGGTGLNLTGADTVVHFDPWWNPAVEDQATDRAHRIGQTKNVNV
                     YKLIAAGTVEEKILELSAKKRALVSNVLSTEGSPLKGLTKADVENLFSD"
     gene            complement(8400..9116)
                     /locus_tag="VFG22_00200"
     CDS             complement(8400..9116)
                     /locus_tag="VFG22_00200"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET6414682.1"
                     /translation="MIESITIPVTGTRGHEAALSLPTAGDAPFPGVVVIHDIYGFSAD
                     LKRHCQRFADAGYVAVGPDLYNGGQPGCVVKTLTSMATGKGFAYEVIDAARELLAARD
                     DVDPEQIAVAGFCMGGGFALIAAADQSFAVAAPFYGAVPRKASRLKNLCPTIAQFGAE
                     DRVFLPHAKRLVRHLEELGIEHEVHIYEGVGHSFMNQHEGRLATLGRHTPIHAFYEPE
                     TEAKAWGKLLDFFERHIGTA"
     gene            complement(9170..9559)
                     /locus_tag="VFG22_00205"
     CDS             complement(9170..9559)
                     /locus_tag="VFG22_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414683.1"
                     /translation="MKRLVATLGILLMTQSAAADSYEEHDYILNCSGCHRMDGSGSRT
                     VPPLDAMPELGGKPGAREYWIRVPGAAQAPLSDARLAALMNWLVERFTGNPVEPAYTA
                     KEVGELRRNPLRDPIQGRKAILRLPAR"
     gene            complement(9556..10095)
                     /locus_tag="VFG22_00210"
     CDS             complement(9556..10095)
                     /locus_tag="VFG22_00210"
                     /inference="COORDINATES: protein motif:HMM:NF014969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0009308 - amine metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylamine dehydrogenase light chain"
                     /protein_id="HET6414684.1"
                     /translation="MSRLDHWFEGMARQSARRVSRRHWFSRFGAVLVGGAALPLLPVA
                     RAAEKHTLGEDGVKGPEGDPTRCEYWRHCAIDGFLCSCCGGSHTACPPGTEMSPVTWI
                     GTCRNPADGKDYIISYNDCCGKTYCGRCMCNRNEGERPLYHWYRNNDINWCGGAKAQT
                     YSCSTAVVVKTAKEAQPEK"
     gene            complement(10092..10691)
                     /gene="mauD"
                     /locus_tag="VFG22_00215"
     CDS             complement(10092..10691)
                     /gene="mauD"
                     /locus_tag="VFG22_00215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylamine dehydrogenase accessory protein
                     MauD"
                     /protein_id="HET6414685.1"
                     /translation="MIDALVISNGVLWLLVIGLALTVFALTRQIGLLHERISPVGALS
                     PEAKVRVGDKAPELALTDIHSRPVQIGGASADGSRTLLFFLSPTCPVCDTVLPTVERV
                     ARTEVPRVRVVLASDGELGEHQAFAAEKRVDHLPYVISAPLGMSYGVAKLPYAVLIDE
                     HGIVVAQGLVNTREHLESLFEAQRHGVASIQDYLEEKAR"
     gene            complement(10688..11227)
                     /locus_tag="VFG22_00220"
     CDS             complement(10688..11227)
                     /locus_tag="VFG22_00220"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HET6414686.1"
                     /translation="MIDPVISLTIRLALALLFTAAAWHKLSDHVRFVATVRAYRLLPE
                     WLLSLAAWLFPAVELGIAIGLLYPPATEATVFAAVLLLSLYSFAIWQNLTPDGRQIDC
                     GCFASSAKVPPSGWLVARNVVLMLATCVLVMPNRARALIWVDGLTVFAALFTLWVLWN
                     AGQRLASTGPALRRRGAAR"
     gene            complement(11224..12459)
                     /locus_tag="VFG22_00225"
     CDS             complement(11224..12459)
                     /locus_tag="VFG22_00225"
                     /inference="COORDINATES: protein motif:HMM:NF018172.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030058 - amine dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amine dehydrogenase large subunit"
                     /protein_id="HET6414687.1"
                     /translation="MIHGAKLWSQLCLAGCLLALSADAGADLPPEEIGQVARLPELVG
                     DHWVWVPDRLFRHSPLFDGDSGKMLGAIDSGMQITPKSPLWSRSRHEIYNIDTVYSRG
                     HRGERHDYVVIYDSHSLEVKGEVEIPPKTMDTGTAIALVGMLDGERFLVALNQSPGSS
                     VSVVDLVARRLVAEVQTAGCAAVFPAGPTRFGMLCGNGTAITVHLKENGELDRIARSA
                     PFFDVVADPVSEKGVRDGATWWFPSFEGQLYEVDFSDDAPMPKEPWSLFSESERSDQW
                     RVGGAQHLTYHRATKRLYSVVHQGGPGSHKDPGTEIWVYDLPAKKRVDVFEAPNLIPP
                     FLGPQAGFDAKGMIGKILNFVLPNLGVHSIAVTQDSAPLLFVRHVDFGAVGVLDAQSG
                     EHLRDISEAGLSGVMLVLP"
     gene            complement(12502..12813)
                     /locus_tag="VFG22_00230"
     CDS             complement(12502..12813)
                     /locus_tag="VFG22_00230"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HET6414688.1"
                     /translation="MPETLDCLTCGACCRTGADGRILIPPEDLLRWRKMGRQDIALAV
                     QPGHFGMLGFATSEEGACVHLGTEDSPHACQIYEIRGTTCREFEKGSPQCLEFRRDAG
                     H"
     gene            12891..13385
                     /locus_tag="VFG22_00235"
     CDS             12891..13385
                     /locus_tag="VFG22_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414689.1"
                     /translation="MWKSLLFASVILCACQGKEQAAEVALPKVAPDTVVVSWSVISRT
                     ADRDSIHVALDASRKLHVTNKAPNGTMMSIARTISDDDYAKLVGSLRALDCCSLRSTT
                     EERASPSEAKPQLEIDFGDVSCEVALWDREWREGLARECGFTVARLHGAGFVPDPPVD
                     EHSP"
     gene            13403..14968
                     /locus_tag="VFG22_00240"
     CDS             13403..14968
                     /locus_tag="VFG22_00240"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6414690.1"
                     /translation="MPSKYFCPNCNAEFVLQEATEEPRCPRCMRHGLPEPMGDPRAEN
                     RLRSPWLLLIVLVIVSAGIGLGLYLSARTVLEETPPLRPLEPRELSAYLERDQIASGP
                     YESMLVLSGETGTWPSAPAEIASAMHAQSASWSLERPLTREVLTADQTLALLDAHDEH
                     VPVYPLELATAMTSLLREHGMKAMVAEVWELEGARAPADPSGVLGYFVTAVYDDPDAS
                     EPLAYFDPWGGRGEVELSSVRVLRDTEVLAAALGTEAARIFARSGDAPRALAMAEAAL
                     LLDPVSPMLRAVNATILADSGGLAEAIKEFEAAAQLRSDGPRQLSLAQLHLAQAGILE
                     MNAEPAAAEAQFGEANRIVTDVIERWPRFGRAHVVLATIYLGMDQLERAQVELEAAES
                     LAPDAPILWAVWAQYDFQRDDMIGAAAKIQRAVGLDPENWQLRLQAGRVLRNAGQDEA
                     AKENLAKAMEMVQPAKRDEVRQFIERMMGAGANPPSAPPQAVPEDPALKLGDPSNLRL
                     RDPNQSLELDLGE"
     gene            complement(14973..15626)
                     /gene="yihA"
                     /locus_tag="VFG22_00245"
     CDS             complement(14973..15626)
                     /gene="yihA"
                     /locus_tag="VFG22_00245"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HET6414691.1"
                     /translation="MSEQGRYNAGMDVLDAEFVAGATGLAQLPAPTFAEVAFAGRSNV
                     GKSSLINSLVRRKKLVRTSGAPGCTRAINIFRVRLRTDEEEAHLDLVDLPGYGYAQRS
                     KAERRSWGPLIEGFLRERPGLRGAVVIVDIRRGVQDDDVQLLDFLESIEVTPILVATK
                     LDKLPASKRKLAIAALRRELGRPVLAYSSVTGDGWEALWERLLSVSSIERQEDAAEG"
     gene            complement(15623..17215)
                     /gene="murJ"
                     /locus_tag="VFG22_00250"
     CDS             complement(15623..17215)
                     /gene="murJ"
                     /locus_tag="VFG22_00250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HET6414692.1"
                     /translation="MSEKPVRTVGGDIVRRAGVVAAGTLSSRILGAVRDAVVAAVFAL
                     AATDAFWLAFTIPNALRVLLGEGAVSAAFVPVFTEVRERESMARAREFFANLVGAMAL
                     VLLAVTVLGVLFAPWIVKGYAWGFQRDEALFDTTVALTRLLFPYIFLMGISALMMGAL
                     YASKRFVASSFAPAFLNICLIAAAFLLAPRLRELGWPGIFALAVGALLGGILQIAAQV
                     PSLRREGLLVRPKIGFSDIYVRKCIRLMGPLLAGLGVYQLNVLLSRLFASFLPTGSVS
                     YLYYGQRLAEIPQGMFALAIASAALPSLSHAVAHGDEEEAKRLFRHALRLSLFVAVPA
                     AVALVVLAEPTATVFFGRGRYDALAIEETTRSLVWQAGGIWAVASVRTIVPMFHAHSD
                     TRTPVIASAFNLVAFIGLSLLLMEPMQHAGLALATTAAAVLQLFVLLWLLRKRTGHLG
                     LVEVSGSLLRVVVASTAMGGVVWAGARAGNWAKGGNDPLNLLVFTATAIVGAVTYFAV
                     AAALGSPELSDLKAALVRRVRA"
     gene            complement(17267..18127)
                     /locus_tag="VFG22_00255"
     CDS             complement(17267..18127)
                     /locus_tag="VFG22_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HET6414693.1"
                     /translation="MSETDSVMRAMTNDGAFRIVAARTTDTVRGVIQAQKLSGRIAAD
                     LADLVTAAVLYRETMAPSLRVQCIVRFDEDAGQLVADSHPDGWTRGLLQRGVGDAEPD
                     VRRTRATLQMMRTLPNSDLHRGVVEVPGNGNLSEAFMRYMQLSEQIVSMISLGSAVAG
                     ETPAAGGYVVQLLPEAAEADAAMETMTRRLEDFVEIRERLQQSDGSPARLIEQIFEGM
                     PFTWLHSSEIRFGCQCSAIRVMTTLSILDRSDIQELVDEGEPLSVGCDYCGASYTVEI
                     AQLQGLLSAS"
     gene            18191..19222
                     /locus_tag="VFG22_00260"
     CDS             18191..19222
                     /locus_tag="VFG22_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HET6414694.1"
                     /translation="MTTHQIVLIPGDGIGPEVAEATTRVLEAAGAPLAWAEHHAGVAA
                     LERHGEVLPEATLDAVRDRGIALKGPCTTPVGKGFTSVNVQLRKRLDLYAAVRPVRNL
                     PGITTRYEGVDLVVIRENTEGLYSGIENEVTKGVVTSLKVATETASQRIARWAFQYAR
                     DRGRQKVTVMHKANIMKLTDGMFIRCARQIHDDEGFSDIEYEEVIIDAGCMKLVQDPT
                     QFDVLLLENLYGDVLSDLCAGFVGGLGVIPGANIGNDYAVFEAVHGSAPDIAGKGLAN
                     PLALLMSAVMMLNHIADTRGDESCRTSAGRIREAYNRALSDGKKTRDLGGNLGTEDFA
                     SAVIERIAG"
     gene            complement(19219..21807)
                     /locus_tag="VFG22_00265"
     CDS             complement(19219..21807)
                     /locus_tag="VFG22_00265"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET6414695.1"
                     /translation="MSRRRQILNWLARLVTERRRLVVWVSVLVTLAFTTQIPKLTADP
                     APESLLASFEGEEYALIQKRFEQWFGKRREAVLILVEADDVLSRESLQQIHDISLYFA
                     DQPWVEKVAGITTLNIPRRVKAAPDADESDLDNLDGLKEVGKPDGEGDLDDLSDLDDL
                     DTLDDTEEVGDAQGDLEDEAFNALLDIVESDPGRFPDGIAKVGPALSAELKTDPIVKG
                     TEVTEEEAAELGAAIANNPLLVGRLIDEDHTVAGIALQLHDLDPKQMRRAMDELREGL
                     ERPEFANIDVHVGGLPYLRSVIVSKMRADNLRLIPLTVLVCMALLMLSFRWVPGVLLP
                     LGAVAITALVVVGGMGLFGEPMNILNNIIVPLLIIIGISDATHLIQRYRDEVHADEDS
                     DRIAAGRRTVRAIAVACMLTSATTAVGLASLVVSKTVMLRHFGLTSGIGVMLSYLVII
                     TFVPAVLTWVAPPKREQVRRERTSMFTTVTGSLVAKLLKWRWAVLGLTAVFTLGAGYV
                     GSQITVDHALLDQFDETDPVYTTTLLIEEKLAGVRPLEVILVSTDPEHFTEPEMIQKI
                     QRIRNWASLRPEVIRTMSYGDMLRESYALLADDPSVRSENFKSAKQVRGLVTMMSQRN
                     PSPLGEWLTEDGTKARVQIKLRDVGAQATMRFLDTLEKMIESELGDDETVSVSFTGEA
                     YTGSRGQAAVVSDLLGSLLVAVLVIFLLLAFFFRSIRLGLLSIPPNLIPLVATMAYMV
                     WREIPLNVSTVIIFSISLGLAVNGTIHVLARFREEMHKGIGREAALVRAARGTGRAIV
                     ISCVTLMAGFSVLLVSSFVPVRNFGELIAITIGGCLVATLIVLPPLLRVAGGDGGVRA
                     GQEDDG"
     gene            21914..22180
                     /gene="rpsT"
                     /locus_tag="VFG22_00270"
     CDS             21914..22180
                     /gene="rpsT"
                     /locus_tag="VFG22_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HET6414696.1"
                     /translation="MANHASAKKRVRQTIKRTARNRNLRTNVRTSVKRVRAAIEAGDA
                     NAAKTALVAAVSRIDGAVSKGIFHRKTGSRYIARLTHQVNTLQS"
     gene            complement(22195..23196)
                     /gene="holA"
                     /locus_tag="VFG22_00275"
     CDS             complement(22195..23196)
                     /gene="holA"
                     /locus_tag="VFG22_00275"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET6414697.1"
                     /translation="MPSIHDVTKRASKGSLDPVYVLVGAERLLIERTVDAIRRAVDSM
                     GAPGFNVEIFDGKGLEAGRVTSAARTLPMMADMRLVLLRNVDAMTPTEQTKLAEYLDD
                     PSESTCFVATASKLDGRAKLSKTAKKKGYLIEASSLRGASLRKFVREEATVREHAIAP
                     QAIEALLDAVGDDLAAIDDAMERVSLFVGAGQRIDADAVMRCVTRIRVETIWTLVDAI
                     GLKDRRKGIAAAQSLLDDREPPLRLLAMVARQLRIVARMREALSEGLRPQDAAKRAGA
                     PPFKAGDLTESARRFTSDSLGQAFTLIARTDRALKSSKRPPDVILQDAVLELCANPQ"
     gene            complement(23200..23736)
                     /locus_tag="VFG22_00280"
     CDS             complement(23200..23736)
                     /locus_tag="VFG22_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414698.1"
                     /translation="MNLRLALAASALAAVVSSVTPWQGADAASGLRAKIFLTQKGVPG
                     SLSESGVIRFAQSNKATRLVESNDAPVEDRHWRATLVANFNQPVGDSEFEVLFYDIQT
                     AERKFIAPTMTVFVNDRSQRTIVHKMRLKRPQFEPNRRIEMVVTVRRQEVGRYRFQIL
                     GDRVEHSGEVTFSDDEAR"
     gene            23906..>25425
                     /locus_tag="VFG22_00285"
     CDS             23906..>25425
                     /locus_tag="VFG22_00285"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6414699.1"
                     /translation="MTTVSGGVKGLVARAQREAQRRAQSPSTAYLLLVMLQTGGPMGK
                     LLSELGVKESDLLSAIRVVDSEPTSALELAVERAYRLAKTTGARQVLPAHLLMAIARD
                     SRSAAHRSLEVIGAGSRRVQEAVQVTLEPAEARSEPSSTQIRPTASFAAQTQSPAKPL
                     RPSARRSVDPAPLIELHVESPEDEIVIEAPIRGPANAFEDQDELVVPSSSPFMLDAGK
                     YPTLAKLGRNLTEMASLGRIDEVIGRDDEIERLLDVLARRRSNNPILVGPPGVGKTAI
                     VEGLARRLAQGGDGVRGLEGTILVELSAGGLVSGTGVRGALSERMRRLQFEVKRANGK
                     IILFIDEVHTLFIGGDSPDDLAHELKASLARGELPCIGATTDVEYRKYVERDAALARR
                     FSPIHVSEPSAEDAIEILHGIAPRYEVHHGVAYEPSAVECAVELSVRYMSEQTLPDKA
                     IGILDLAGARARRRGGAVVDREAIAQVVAEKVRVPVERILVTDSQKLLELEQHLGE"
BASE COUNT         4838 a         7809 c         8219 g         4559 t
ORIGIN      
        1 cgaagctcgg cgagggcgaa gacggactgg tgctttcggg gcacaccgac acggttccct
       61 acgatgccga tcgctggagc gacgatccgt tcgtcctcac cgagcatgac ggaaagctgc
      121 atggtctcgg cacagcggac atgaagggct tctttgccgc cgcccttcat gcgatcgcgc
      181 gcttcgaacc cggggcgctg cgccggccga tcaccatcct gggcacggcc gatgaagaga
      241 gcaccatgaa tggggcgcgc gcgctcgcaa agaagggcga gcgcctcggg cgctatgcga
      301 tcatcggaga gccgaccgga ctcgcaccga ttcgcaagca caagggagtg ttctacgttc
      361 aggtcactgt gtcgggaaag tccggacacg cgagcaaccc cgcgctaggg gtgaacgcca
      421 tcgaaggact gcagctcgtt ctcgccgctt tgcagagctg gcgggatacg attggcgcgc
      481 gctaccacga cgaggacttc gaggtcccag gacccaccct caatttcggg cgtgtgagcg
      541 gtggagacag ccccaatcgg atctgtgcgg agtctaagct cactctggac gttcgtctgc
      601 tgcccgggat gcagcgatcc gaagtgaccg ccgagcttcg cgacgtcgtt gccaacgccc
      661 tcgacggagg tccatggggt ttcgacgtga agggggtggg cggctacgca gacgcgttcg
      721 gaggtccgag cgacgcgcag ttctgcaccg cggtcgagac cttgtgcgga tgttcagcca
      781 ggaccgcgct cttcggaacg gaagcgccgt ttctcacgga attggggctg cagacgctgg
      841 tgctcggcgc cggggacatc gcggttgcac atcaacccga tgaatatgtc accatggcgg
      901 ctatcgagcg tgccaccgac gtgtacgcga aactgatcaa acgcttctgc atcgagccgg
      961 catgagcccc caagtcgacc cattcgtcca acacttccga gcgagcgcgc cctacatcca
     1021 cgcgcaccgc gggcgtacgt tcgtgatcat gtttggcggc gaggcagtcg cttcgaagga
     1081 actgcgcacg ctgctctacg atgtggcgct gttgcactcg ctgggcgtgc ggatcgtgct
     1141 ggtcgcaggg gcgcgtcctc agatcgacga agcgctggcg cggcgcggtg agaagcccat
     1201 gtacgagggc ggcctgcgag tgaccgatga agtcgcgctc gagtgcgtca aagaagccgt
     1261 gggcgcgacc cgcgtcgagc tcgagtcctt gctttcgatg gggttgccga actcgccgat
     1321 ggccggggca cgactgcggg tcgctgcggg caacttcgtg atggcccagc cggtgggcgt
     1381 cgtcgacggc gtcgactatc aatacacggg caagccgagg cgcatcgatg cggaggcaat
     1441 tggtcagcgg ctcgacgatg gtgcgatcgt cgtcctcaat ccgatcgggt attcgtcgac
     1501 cggagaggcc ttcaacatca gcagccacga tgtggcagcc gcgaccgcga tcgcgcttcg
     1561 cgccgacaaa ttgatcggtc ttctcgaagg gccgggcgtg ctcgatgccg aggggttggc
     1621 tcagtcccag ctcagtccga acgaggccga acgtttgctc cagtcaggag ccgatctcgg
     1681 tcacgacgtg gaacgacatc tcggtgcagc catcacggcg tgccgaggtg gagtgccgcg
     1741 cgcgcatctc gtctcccgtc agagcgacgg ctccttgctg cgcgagctct tcacccgcga
     1801 tggcattgga acgctagtga cgtcggagcc cttcgaaggg gtgcgccatg cggcccaaag
     1861 cgacattggt gggatcctcg ccctgatcga gccactcgaa gagcagggca tcctggtgcg
     1921 gcgttcgcag gagatgctcg agaacgacat cgatcggttc accgtgatcg agcgcgacgg
     1981 catggtggtc gcctgcgcgg ccctctacgg ctatccagac gattcgatcg ccgagctcgc
     2041 ctgcctggtg gtccatccgg actatcgaac ttccggccgt ggcgatcagc ttctgcagta
     2101 cctcgagagg caatgtgccg ctcagggtct caagcgtcta ttcgtgctca cgacgcagac
     2161 ttcgcactgg ttcagggagc gtgggttcga caattgcgag ctgggcgagc tccccgaggc
     2221 ccgtcgctcg ttgtacgact tggggcggcg ttcgaagatc cttctcaagc cgctcgattg
     2281 acgcatctgc cccaaactac gggcgttttc gttcatgctt ggatgagtcc gcgaatcgtg
     2341 ctactcgtcc ccgaattcgc ctaaacggga cctgattcgt ccttgatacg cgcaaaatga
     2401 agcaggccga gctcaatcca cgccctgaag cggacgcgcc acccgcagac gcggaaatgg
     2461 acgaaatcgt cgctcaggaa gaaaaggtcc tagcgcgggt gcaccgaacc gttgcggcga
     2521 ggcgccccag ccatcgcggg cagctgatcg actacgacgc cgagttgatc gcgctccgcg
     2581 atcagattcg agaggcgcgc ctcgaagaca ttccgcccct ggtcgaggag atggagcgac
     2641 tgcagcaggt cgcgcagcgc agggcgaagg tgaccgaagg ctcggtcgac cgtggttcgc
     2701 cgtacttcgg acgcatgatc ctcgaggaag agggtcgcaa gcgcgagatc ctcataggcc
     2761 gcagcaccta cctggacccg aagaccggcg tccgcatcgt cgactggcgg gacgcacccg
     2821 tgagccgtgt ctactaccga tacgaagagg gcgacgacta cgacgagatc ttcggtgacc
     2881 gcgaagtcga aggcgaggtg ctggttcggc gcaatctgtc gattacgacg ggcaagcttc
     2941 gacgcatcgg cgctccgcag ggaacgtttc ggcgctcgcg cgacgacata tggcggcggg
     3001 tcgcggtgct ggagacgaag ctctcgggcg gccaaggcgc cgccatgcgt cctgagtcgc
     3061 accatcgtcc cggggagctc ggaatcggtg aggacgacat cagggaggac aagcaccttt
     3121 ccgagatcac cgcgctcatc gacccggtgc agttcgagct gatcagccgg ccgacctccg
     3181 ggctcgtcgt gattcaaggt ggcgcaggta gcggcaagac cacgattggg ctccatcgac
     3241 tcgccttcct cgcattccag gatccggatc gctttcgttc cgagctcatg ctcgtcatcg
     3301 tcttcaacga cgcgctcgtg cgttacattt cgcgcgtcct gccggcgctc ggggtggagg
     3361 acgtgcccgt tcagaccttc cagcgctggg ccgagcagca gcggcggcga cactttccca
     3421 agctgacgac gagatacgtg gaagacacgc cgacgcacgt cgttcgaatg aagaagcacc
     3481 ctgcgatgct gcgcgcgatc gatcagtacg tcgccgagct cagcgagaag gcagaggccg
     3541 agctgcttca ggccaccgat caatccgacg atagcgctcg agtgagcaga atttggcgcg
     3601 gaaccgccgc ccagcctcta ggggtgcgac tcgaggtgct ttctcagtgg cttcaagatc
     3661 ggaccggaga cgctcagcag ctctcggcgg gcacccgaca tgcgttgggg cgcatttgca
     3721 atcgctggac ttcacgggca cgtgacgtcg tcacggcctg ggccgacttg ttgaccgacc
     3781 tgcagcggct gacgggtgtg ttcaaccgat gggccccgga tgctctcacg gacaaagaga
     3841 tcgaatgggc gcactcctgg tgcagccgtc actcgacggg cgtgttggcg gcgtacgaag
     3901 aatcgatcga agacgacgat tcgcaaaccc ggagcacggg cgtggacggt ctcgacgaag
     3961 aagagcctgc ggagctcgac gtcgaagatg acgccctctt gcttcggctc ttccagacgc
     4021 ttcggggtcc gctttcgggc cggcggggtt ctctccgcta cgagcacatc ttcgtcgatg
     4081 aagcccaaga catgagccca gtcgagctcg cggtcgtgct cgacacggcg acggagcagc
     4141 aatcggtcac gctcgcaggc gacatcgccc agaagctcca tttggacaat ggcttcagcg
     4201 attggcgaca cgtgctcgag gagctgaagc tcgaccacat cgaaatcgag ccgctgaagc
     4261 tgagctaccg gtccacgcac gagatcctcg acttcgctac cgatgtgctc ggaccccttc
     4321 gtaacgaggt gtccggagtc gcgacccgct acggcgcccc ggtcgagctg ttccagttcg
     4381 gaggcagcgg cgaggcggtg ggattcctct ccgaagccct ccgggcgctc gtgagctcgg
     4441 agccgatgtc ctcggtcgcg gtcatcgccc ggtaccccga gcaggccgac ctctattaca
     4501 aggggcttcg ccacggggag gtgcccaacc ttcgacgcat cgccgagcag gactttccgt
     4561 tccgcccggg tgtcgacgta acggacgtga ggcaggtcaa gggcctggag ttcgactacg
     4621 tggtcatcgt ggagtgcaat aggggcgagt tcccggccga tgacgagtcg cgccacctgc
     4681 ttcacatcgc cgcaacccgg gctgcgcacc agctctgggt gttctccacg ggacggccct
     4741 cccttctgct gcccgaatcg ctcgttcgtc cgcaggacta gcgctttgcc ccaaggggct
     4801 gtatccagtt gcagtctgcg atggggcgtg taagatgccc cacgtagcgg cggttgcggc
     4861 ctacaccggg gattcacggt agaacgagca acgacgccgc taggggaaaa gacgcaacgc
     4921 gcctagttcc gtgtaggtcc tactagcctc caaccacaac ccactcaggc tgatctcagc
     4981 ctccatgcga tggcagatac tgctcttcaa gatacgctcg agaacgaagc cagcccggag
     5041 ctcaatccgg atgcgcttgc ggacgacaat gcgccgacct ggcgtgaacc cacgaccgaa
     5101 gtcgccaaga agcttgcttc aatgagcgac cgcgcgattc agcgggtcac cgggggcaac
     5161 gcgttcctgc gtggtcgtct ctacgcgcgg cgaaaagcgg tcgaaagtct caaagaagac
     5221 gggcattctg cgcgcggtga gatttcggta cgcacggccg acgagccgta ccgaacggcg
     5281 atcagctaca aggaggagag cgacagctgg gaatcgagct gcacttgccc cgggtggaga
     5341 gggccgaccg ggcattgcaa gcacgtcgcc gcggtgatgg tcgccttgcg tgacgaggtt
     5401 cgccctccgc gctccaaggg ccagccgcag caaaccaaga agaagaagtc cgagcccttg
     5461 catgtaccgg ggacggtttc cgttggcggc aagaggcggc gctcccgccg cagacggcgt
     5521 ggcgcaaccg gcggtgaagg gggcgggctg gagatcctgt cgccacgcga gatgcaggcc
     5581 aagcgcggcg aggcccgcgg gcccatggat gcctggctgc ccgatgaggc cttggataag
     5641 cctctcgatt tcgagtaccg catgacggtt cgcccggctt ccatcacggt gacgccggtt
     5701 ttggcgggct cgcgaagcgc agtgcccatt gctgatgctc taggcgcgtt caacatggtg
     5761 tccatcgacg agcgaccgct gtttcgcgcg ctcgcccgga attccaatcg cggccagacc
     5821 accacggccg agctgcgcgg tgaagatgcc gcggaggttc tcgagatgct gagcgaccgg
     5881 cgtgtgctcc tcgagcctgc atcgatggag ctccggtttg cgaacgaggc gttgaaaccg
     5941 cgcatcgagc tcgatccagc aaacggcgac agcctgcgcg tgcgcgtcgt gttctatttg
     6001 gagagcaaca agcgtcgttt cgccttgtcg agtggggcct ggttcgaagg gacgcccggt
     6061 tggcagatcg acaccaccga aggggtcgcc cggcccgtga gtgagctggt gactccggcc
     6121 tggcttcagc ggctctaccg ctcgccagcc atggtgcagt ctcacgacga tcttcccgcc
     6181 ttgctgggcg aatacattcc gcgaatctcg gcatcgctcg gagcagatct tcccgacttg
     6241 agctcggtgg ccgatctggt cgatcagaag ccgcgcttcg agctcaaggc gaatggcgac
     6301 atcatcgacg cgcgcgttcg gctgtacgtc cgctacgaga accaggagtt cgaggttcct
     6361 cccgacgatt tcccttcacc gctggcgttc cttccgccca agcggggcga gagccgaccg
     6421 cgtgtcgtgc gccgtgacgt cggggcggag atggcgggga tccaggcgct cctcaaccag
     6481 aacctggagg tttccgagtc tggcgatgag ctcgtggcca ccggtgatga tgcgatcgcg
     6541 ttttggacca acgggatcgg tgagcttccg gaggaatggg atcgcttcat ccccgacgac
     6601 ttggtcgacg tgaccattcg agacgagtcg gtgtccccgc agatgcgcgt ttcgagtgga
     6661 gtcgattggc tcaatctcga catgaagttc gaatcgggtg gcgccgtggt gcgcgaagaa
     6721 gagctccgag cgtgtctcga gcacgggcgt cggctcgtac ggctcgaaga tggcaccttc
     6781 gcgcccgtcg acccggagaa ggtcggcgac gtgctcacgc gaatggcgga gatctacgcc
     6841 acggcaggca agagcgaaaa gttaccgctc tcgcaggcag gacgggtgca agacctcttg
     6901 aagatggtcg aaaacgccaa ggtctcggcc accgcgaaga ccttgttcaa gaagatcgag
     6961 cacatcgacg agatcccgag catcgcgaag ccgcgcacgc tcaaggcgga ccttcgtccc
     7021 tatcagaagg acgggttctc ctggctcgtc tttcttcacg agctcaacag tggcggcatt
     7081 ctggccgacg acatgggcct cggtaagacg attcaaacga ttgccctcct tctctgggcg
     7141 aagagcaagt ccaagcgcaa gctcaacctg gtcatcgcgc ccacctcggt cgtgccgaac
     7201 tgggagcgtg agatcagcaa gttcgcgccc ggcctgaaga ccgtcgtttg gcaaggaccc
     7261 aaccgaaatc agcaagcctc cgacctcgag aaagccgacg tgatgatcac gagctacgcg
     7321 ctgcttcggc gcgacgagga gctcttgcag gctctcgacc tcggctatgt gatcctcgac
     7381 gaggctcagc acatcaagaa ccccatgagt cagaccgcac ggtcggcgaa gaagatccgc
     7441 agtgagcgac gcctggcgct gacgggcaca ccgatcgaga atcgtctgag cgagctctgg
     7501 agcatcttcg atttcgtatc accggggctg ctcgggccgc tcaaggcgtt tgaagagcgg
     7561 attgcgaggc cgatcgaccg aggggacatg gaaacggcgc agcgactgcg ggcgaccatc
     7621 aagcctttcg tcatgcggcg actcaagcgt gatgtcgccg cggacctgcc ggacaagatc
     7681 gagcaggaga tgatcgtgcc gctcgccgaa gagcaggcca agctctacaa gcaagttctg
     7741 ggtcaggtgc gcaagagcgt gttgagcgaa gtggagaaga agggcgtcag caaggcgcag
     7801 attcaaatcc tcgctgcgct gacccgcttg cgacaagtgg cctgcgaccc gcgcctgatg
     7861 aagctgccgg ataccgattt cagcgcggac gatagtggca agctcggcgc gttgctcgag
     7921 atcatcgacg aagcggtgga tggcgaccac cgcgttctgg tcttcagtca gttcgtggag
     7981 atgctcaccc acatccgaac agcgctcgac agcgccggcg tgaaatacga gtacctcgac
     8041 gggtcgacga agaatcgcct cgagaaggtg gaccacttca acgacgattc gagcgttccg
     8101 gtcttcctga tttcgctcaa ggcgggcggc accgggctca acctcacggg cgccgacacc
     8161 gtcgtgcatt tcgacccttg gtggaaccct gcagtcgaag accaggccac tgaccgagcg
     8221 caccgcatcg gccaaaccaa gaacgtcaac gtctacaagc tcatcgcggc tggcaccgtc
     8281 gaggagaaga tcctcgagct ctcggccaag aaacgtgcct tggtcagcaa tgtcctctcg
     8341 accgagggca gcccgctcaa gggcctcacc aaggctgacg tcgagaacct cttcagcgac
     8401 taagccgtcc cgatgtgtcg ttcgaagaag tcgaggagct tgccccaggc cttggcttcg
     8461 gtttctggct cgtagaacgc gtggatcggg gtgtggcggc ccagggtagc gagcctgcct
     8521 tcgtgctggt tcatgaacga gtggccgacg ccttcgtaaa tgtggacttc gtgctcgatg
     8581 ccgagctctt cgaggtggcg cacgaggcgt ttcgcatggg ggaggaagac gcggtcctcg
     8641 gcgccgaact gggcgatggt ggggcagagg ttctttagac gcgatgcttt gcgaggaacg
     8701 gcgccgtaga agggggcggc caccgcgaag gactggtcag cagccgcgat cagggcgaag
     8761 ccgccgccca tgcagaagcc ggcgacggcg atctgctcgg gatcgacgtc gtctctggcg
     8821 gcgagcaatt cgcgtgcggc gtcgatcact tcgtatgcaa aacctttccc cgtcgccatg
     8881 gacgtcagtg tcttgacgac gcatcctggt tggcctccat tgtagaggtc ggggccaacc
     8941 gcgacatagc cagcgtcggc gaagcgctgg cagtgacgct tcagatccgc actgaagccg
     9001 tagatgtcat gaatgacgac gacgcccgga aagggcgcgt cgcctgcggt cggtagcgac
     9061 agtgcggcct catgtccgcg cgttccggtg acggggattg tgattgactc gatcatccgg
     9121 gagagagcat gtgccagtgc cagtgccagt gccagtgcca gtgccagcgt cagcgggcgg
     9181 ggagacggag gatggccttg cgcccttgga ttggatctcg cagagggttg cggcgcaatt
     9241 cgccgacttc ctttgccgtg tacgcgggct cgaccgggtt tccggtaaac cgctcgacca
     9301 gccagttcat gagcgccgcc aagcgagcgt cggaaagcgg agcctgagcc gcccctggga
     9361 ctcgaatcca gtattcgcgc gcgccgggct taccgccgag ctccggcatg gcatcaagag
     9421 gaggcacggt tctcgatcct gagccgtcca tccgatgaca tcccgagcaa ttcaggatgt
     9481 agtcgtgttc ctcatatgag tcggcggctg ccgattgcgt catgagcaag atgcccagcg
     9541 tagcgacgag gcgcttcatt tctctggctg ggcttctttg gcggtcttga cgaccactgc
     9601 ggtcgagcag ctgtaggttt gcgccttggc accgccgcac cagttgatgt cgttgttgcg
     9661 gtaccagtgg tagagcgggc gttcaccttc gttgcggttg cacatgcacc ggccgcagta
     9721 ggtcttcccg cagcaatcgt tgtacgaaat gatgtaatcc ttgccgtctg cgggatttcg
     9781 gcaggttccg atccaggtaa cgggggacat ctcggtgcca ggcgggcagg cggtgtgcga
     9841 gccgccgcag caactgcaga ggaagccgtc gatggcgcag tgacgccagt actcacagcg
     9901 ggtcggatcg ccctccggcc ctttgacacc gtcttcgcca agcgtgtgtt tctcggccgc
     9961 gcgtgcaacg ggaagcaagg gaagagctgc gccgcctacg agaacggcgc caaagcgcga
    10021 gaaccagtgt cgacgggata cccgccgagc ggactgcctc gccatcccct cgaaccaatg
    10081 atcgagtcga ctcatctcgc cttctcctcg agatagtcct gaatcgacgc aacgccgtgt
    10141 cgctgcgctt cgaacaggct ctccaaatgc tcgcgcgtgt tgaccaagcc ctgcgcaacc
    10201 acgattccgt gttcgtcgat gagcaccgcg taggggagct tggcgacgcc gtagctcatt
    10261 ccgagtggcg ccgagatgac ataggggagg tggtccacgc gcttttcggc ggcgaatgcc
    10321 tggtgctcgc cgagctcccc gtcgctggcc agaacgacgc gcacgcgcgg cacctccgtt
    10381 cgtgcgactc gctcgacggt gggcagcacg gtgtcgcaaa cggggcaggt cggtgaaagg
    10441 aagaaaagca gcgtccggct cccatctgcc gatgcgccgc cgatctgcac cgggcggctg
    10501 tggatgtcgg tcaacgctaa ctcgggcgct ttgtcgccga ccctgacctt tgcttcggga
    10561 gagagcgcgc cgactggaga gatccgttcg tgaagaagcc ctatctggcg agtgagcgcg
    10621 aagacggtca gtgccagccc gatgaccagg agccacaaga cgccattgga gatgacgagg
    10681 gcgtcgatca tcgcgcggcc cctcgtcgac gcagtgcggg tccagtgctc gcgaggcgtt
    10741 gtcctgcgtt ccagaggacc catagtgtaa agagcgctgc aaagacggtc agaccgtcga
    10801 cccaaatgag cgcacgcgct cgatttggca tgacgagaac gcaggtggcg agcatcaaca
    10861 cgacgttgcg cgcgacgagc cagccgctcg gcggtacctt cgccgagctt gcaaagcagc
    10921 cgcagtcgat ctggcgtccg tccggtgtga ggttttgcca gattgcgaag ctgtagagag
    10981 agagcagcag gacggcggca aacacggtgg cctcggtcgc gggtgggtaa agaagtccaa
    11041 tggcgatccc gagctcgacc gccgggaaga gccacgctgc cagggacagc aaccactccg
    11101 gcaacaaacg ataggcacgg accgttgcga cgaagcgaac gtgatccgag agcttgtgcc
    11161 aagcggccgc ggtgaacagg agcgcaagcg ccaggcgaat cgtgagcgag atgaccgggt
    11221 cgatcatggc agcaccaaca tcacgcctga gaggcctgct tcgctgatgt cacggaggtg
    11281 ctcaccgctc tgtgcatcga gcacaccgac cgcgccaaaa tcgacatgcc gaacgaagag
    11341 cagcggcgcg gagtcttgcg tgacggcgat gctgtgcacg cccaaattgg gcaagacgaa
    11401 gttgaggatc ttcccgatca tgcctttggc gtcgaagccc gcttgagggc cgagaaaagg
    11461 gggaatgagg tttggcgcct cgaatacgtc gactctcttc tttgcgggaa ggtcgtagac
    11521 ccagatttcg gtgcccgggt ccttgtgtga gcccggcccg ccctgatgga cgacggagta
    11581 gaggcgcttg gtggcgcgat ggtaggtcag gtgttgcgcg ccgccgactc gccactggtc
    11641 gcttcgctcc gattcgctga agagcgacca gggctctttg ggcatgggtg cgtcatcgct
    11701 gaaatcgact tcgtagagtt gcccttcgaa cgacggaaac caccaggtcg cgccgtcgcg
    11761 cacgcctttt tcggagaccg gatcagcgac cacgtcgaaa aatggagcgc ttcgcgcgat
    11821 tcggtcgagc tcgccattct ctttgaggtg cacggtgatc gccgtcccgt tgccgcagag
    11881 cattccgaag cgtgtcggac cagccggaaa caccgccgcg catcccgccg tctggacctc
    11941 cgcgaccaaa cgccgggcaa ctagatcgac gaccgaaacc gatgatcctg gcgattggtt
    12001 gagcgcgacc aggaagcgct cgccgtcgag catgccgacg agcgcgatcg ccgttcccgt
    12061 atccatcgtc ttgggaggga tctcgacctc gcccttcacc tcgaggctat gcgagtcata
    12121 gatgacgacg tagtcatgcc gttcgccgcg atgccctcga gagtaaacgg tgtcgatgtt
    12181 gtagatctcg tgtcggctgc gagaccatag cggtgacttg ggcgtgatct gcatgccgct
    12241 atcgatcgca ccgagcatct ttccgctgtc tccgtcgaag agcggcgaat gacgaaacaa
    12301 gcggtcgggc acccagaccc agtgatcgcc gaccagctcg ggtaagcggg caacctggcc
    12361 gatctcttcg ggcggcagat ccgcgcccgc gtctgccgaa agagcgagta ggcaacccgc
    12421 taggcaaagc tgagaccaga gcttcgcccc gtggatcatg aaagactagg tacccgatct
    12481 ctcaaccatc tggcaatacc ctcagtgccc ggcgtcgcgg cggaactcca ggcattgggg
    12541 actgcctttt tcgaactcgc ggcaggtcgt cccgcggatt tcgtagatct ggcaggcgtg
    12601 ggggctgtct tcggttccca ggtggacgca ggcgccttct tcactcgttg caaagccgag
    12661 catgccgaag tgacccggtt ggaccgccag cgcgatgtcc tgccgcccca tcttgcgcca
    12721 gcgcagcaga tcctcgggag ggatcaggat tcggccatcg gccccagtgc ggcagcatgc
    12781 accgcaggtc aggcaatcga gcgtctcggg catcactcgg agattatggc tcgggcgagg
    12841 ccaggcgcac gcgctagact tcgcgcagga gtcgtgatct agtgcgcgcg atgtggaagt
    12901 cccttctctt tgcttctgtg attctctgcg cctgccaagg aaaagaacag gccgcagaag
    12961 tcgcgttgcc caaagttgcg cctgacacgg tcgtggtgtc gtggagcgtc atcagccgaa
    13021 ccgcggacag agacagcatt cacgtcgcac tcgacgcgag tcgaaagctc cacgtgacca
    13081 acaaggcacc caacggcacg atgatgagca tcgctcgtac catttcagac gacgactatg
    13141 cgaagctcgt aggaagcctt cgcgcgctcg actgctgctc actgcgctcg acgacggaag
    13201 aacgggcaag cccgagcgaa gcaaagccgc agctcgagat cgacttcggc gacgtcagtt
    13261 gcgaggtcgc gctctgggat cgagagtggc gggaaggtct tgcgcgcgag tgcggattca
    13321 ccgtcgcgcg cttgcacggc gcaggtttcg ttcccgaccc gccggtcgac gagcactcgc
    13381 catagacgta tgctccggcc cgatgccgag caagtacttc tgccccaact gcaacgcgga
    13441 gttcgttctt caggaagcga ccgaagagcc ccggtgccca cgatgcatgc ggcacggcct
    13501 gcccgagccg atgggtgacc cgcgcgccga gaaccgtctg cggagtccat ggcttctctt
    13561 gatcgtgctg gtgatcgtct cggctgggat cggactcggc ctgtacttga gtgcgcggac
    13621 cgtgctcgag gaaacgccgc cgctgcgtcc actcgagccg cgtgagctgt cggcctactt
    13681 ggagcgcgac cagatcgcgt cgggccccta cgagtcgatg ctcgtgcttt ccggggagac
    13741 cgggacttgg ccctcggcac ctgccgagat cgcctctgcc atgcacgcgc agtctgcgtc
    13801 ttggtcgctc gagcgccctc tcacacgcga ggttctcacc gccgaccaaa cgctcgcgct
    13861 gctcgatgcg cacgatgagc acgtaccggt ttatccgctc gagttggcga cagcgatgac
    13921 ctcattgctt cgcgagcatg ggatgaaggc gatggtggcg gaggtatggg agctcgaagg
    13981 cgcgcgcgcg ccagccgatc cgtccggggt tctgggctac ttcgtgacgg ccgtatacga
    14041 cgaccctgat gcgtccgagc cgctggcgta cttcgacccc tggggcggtc gaggagaggt
    14101 cgagctgagc tccgttcggg tgcttcgcga taccgaggtc ctggccgcgg ccctcggaac
    14161 cgaggccgca cggatctttg cgcggtccgg ggatgcgccg agagcgcttg cgatggcgga
    14221 ggcggcgctt ctgctcgatc cggtgtcgcc gatgcttcgt gcagtgaacg ccaccatcct
    14281 ggccgattcg ggcggccttg ccgaagcgat caaagagttc gaggcggcag cgcagctccg
    14341 ctcagatggc ccgaggcagc tcagtttggc gcagctccat cttgctcagg ctgggatact
    14401 ggagatgaat gctgagccgg ctgctgcgga ggcccagttc ggtgaagcca atcgaatcgt
    14461 caccgacgtg atcgagcgtt ggccgcggtt cggtcgcgcg catgtggtcc tggcgacgat
    14521 ctatctcggc atggaccaac tggagcgtgc tcaggtggag ctcgaagctg cggaatccct
    14581 cgcaccagac gctcccattc tctgggccgt ttgggcccag tacgactttc aacgggacga
    14641 catgatcggt gcggctgcga agattcaacg cgcggtcggt cttgatccgg agaactggca
    14701 gctacgcctt caggccggcc gcgtcctgcg aaacgcaggg caagacgaag cggcaaagga
    14761 gaatctcgcg aaggccatgg agatggtcca gcccgcgaag cgcgacgagg tgaggcagtt
    14821 catcgaacgc atgatgggag cgggggccaa tcccccgtca gcgcctccgc aggcggttcc
    14881 cgaggatccc gcactgaaac tcggagatcc gtcgaacctg cgcctgcgcg atccgaatca
    14941 atcgctcgag ctcgatctcg gcgaatagaa agtcagcctt ccgctgcgtc ttcctggcgt
    15001 tcgatgctgc tcaccgacaa gagccgctcc cagagggctt cccagccgtc acccgtgacc
    15061 gagctgtaag cgagcaccgg ccggccgagc tcgcgccgca gcgcagcgat ggctagcttt
    15121 cgcttgctcg caggaagctt gtcgagcttg gtcgctacca ggatgggtgt gacctcgatg
    15181 ctctccaaga aatcgagcag ctgaacgtcg tcgtcctgga cgccgcgccg gatgtccacg
    15241 atgacgacgg ccccacgtag ccccgggcgc tccctcaaaa agccttcgat cagcggaccc
    15301 caggaacggc gctccgcctt gcttcgttgg gcgtatccgt atccgggaag gtcgacgaga
    15361 tcgagatgcg cttcctcttc gtcggttctc aggcgcacgc gaaagatgtt gatcgcgcgc
    15421 gtacagccag gcgcgccact ggtgcgtacg agcttctttc ggcgcaccaa gctgttgatc
    15481 aggctggatt tgcccacgtt cgagcgtcca gcaaaggcga cttcggcaaa ggtcggcgcc
    15541 ggtagctgag cgagtccggt cgctcctgca acgaattcgg cgtcgagcac atccatcccc
    15601 gcgttgtagc gcccctgctc gctcacgcac gcacgcggcg taccaatgcc gccttgaggt
    15661 cgctaagctc cggggagccc agagccgccg ccactgcaaa ataggtcacg gcaccgacga
    15721 tcgccgtcgc ggtgaagacc agcaagttga gcgggtcgtt ccctcccttc gcccagtttc
    15781 ctgcacgggc cccggcccaa acgacgcctc ccatcgcggt actggccacc acgacgcgca
    15841 gcaagcttcc actcacctcc accaagccga gatggccggt cctcttgcgg agcaaccaaa
    15901 gcagcacgaa gagctgtagg accgcggcag cggtggtcgc cagagcgagc ccggcgtgct
    15961 gcattggttc catcaggagc aggctcagac cgatgaacgc gacgagattg aaggcgctcg
    16021 cgatcaccgg cgtgcgcgta tcgctgtgag cgtgaaacat cggcacgatg gtacgtacgg
    16081 atgccacggc ccagatgccc cccgcctgcc agacgaggga acgcgtcgtt tcttcgatgg
    16141 cgagcgcatc gtaacggccg cgtccaaaga aaaccgtcgc ggttggctcg gcgaggacca
    16201 ccagggccac tgccgctggg accgccacga agagcgatag acgcaaggcg tggcggaaga
    16261 gacgcttggc ttcttcttcg tcaccgtgcg ccacggcatg gctgagcgag gggagggcag
    16321 ccgacgcgat cgcgagtgca aacatcccct gcgggatctc cgccaagcgt tgaccgtagt
    16381 agaggtagct gacgcttccg gtcggcaaaa aggaagcaaa caatcgcgaa agcagcacgt
    16441 tgagttggta aacgcctaga cctgcgagca agggccccat caagcgtatg cacttgcgca
    16501 cgtagatgtc gctgaagccg atctttggcc ggaccagcaa accctctcgt cggagggacg
    16561 ggacttgcgc ggcgatctgt aggataccgc cgaggagggc gcctaccgcg agggcgaaga
    16621 tccccggcca gccgagctcg cgcagccgag gagcgagcag gaaagctgcg gcaatcaagc
    16681 agatgttgag aaacgccgga gcgaacgacg aggcgacgaa tcgcttgctc gcgtagaggg
    16741 cccccatcat cagcgccgag atccccatca gaaagatgta ggggaagagc aatcgtgtga
    16801 gtgccaccgt ggtatcgaac agggcctcgt cgcgttggaa gccccaggca tagcccttga
    16861 cgatccatgg cgcgaagagg acgcccaaga cggtcaccgc gagaagcacc agagccattg
    16921 cgccgacgag atttgcgaag aattccctcg cccgggccat gctctcgcgc tccctgacct
    16981 cggtgaagac tgggacgaac gcggccgaca cggcgccttc tccgagcaac acgcggaggg
    17041 catttgggat cgtgaaggcc agccagaacg cgtcggtggc ggctagggcg aagactgcag
    17101 cgacgacggc gtcccggacg gcccccaaga ttctcgacga gagtgtgccg gccgcgacga
    17161 cgccagctcg acgtacgata tccccgccta cggttctaac tggcttctcc gacatcggcg
    17221 gaaggatgcg catggggggc gggtcccggc aagaaacccg gtccgctcag ctcgcgctca
    17281 gaagcccttg aagctgtgcg atttccacgg tgtacgacgc gccgcagtag tcgcatccaa
    17341 cgctcagagg ctccccctcg tccactaact cttggatgtc ggaacgatcg aggatgctga
    17401 gtgtcgtcat cacgcgaatt gcgctgcatt ggcagccaaa gcgaatctcg ctgctgtgca
    17461 gccaggtgaa gggcatgcct tcgaaaatct gctcgattag gcgcgcgggc gatccatccg
    17521 actgctgcaa gcgctcgcgg atctccacga aatcctcgag gcgacgagtc atggtttcca
    17581 tcgccgcgtc cgcctcggcc gcttcgggga gcagctgcac gacgtaccct cccgccgcag
    17641 gcgtctcccc cgcaactgct gagcccaagg aaatcatcga gacgatttgc tccgatagct
    17701 gcatgtaacg catgaacgcc tcggaaagat tcccgttccc gggcacctcg acgacgccac
    17761 ggtgcaggtc tgagttgggc agcgttcgca tcatttgcag cgttgcgcgc gtccttcgga
    17821 cgtccggctc ggcatcgccc acgccacgct gcagcaggcc gcgggtccag ccgtccggat
    17881 gggagtcagc gaccagctgc cctgcatcct cgtcgaagcg cacgatgcac tgcacccgaa
    17941 gcgagggtgc catggtctcg cggtagagga cggcagccgt caccaggtcc gccaggtccg
    18001 cagcgatgcg tccggaaagc ttttgggcct gaatcacccc cctgacggtg tccgtggtac
    18061 gcgcggccac gatccgaaat gcgccatcgt tggtcatggc gcgcattacc gagtcagttt
    18121 cggacatgtt ttcctttgaa gcgcattggc tatcgcttgg caagcgctac tcttaggccc
    18181 ggagtagccc atgacaacgc atcagatcgt tctcatccca ggtgacggaa ttggccctga
    18241 ggtcgctgag gccacgactc gcgtcctcga ggcggcggga gcgccgttgg cttgggccga
    18301 gcatcacgcg ggtgtcgctg ctctagagcg gcatggagag gtgcttcccg aggcgactct
    18361 cgatgcagtt cgagacagag ggattgcgct caaaggcccg tgcacgacgc ccgtcgggaa
    18421 gggtttcacc tcggtcaacg tgcaattgcg caagcggctc gacctctacg cggccgtgcg
    18481 tccggttcgg aaccttccgg gcatcactac acggtacgag ggggtcgatc tcgtggtcat
    18541 ccgtgagaac acggaaggtc tctacagcgg aatcgaaaac gaggttacca agggcgtcgt
    18601 gaccagcctc aaggtggcga ccgagacggc gtcgcaacgt atcgcgcgat gggcgtttca
    18661 gtacgctcgc gaccgcggcc ggcaaaaagt taccgtgatg cacaaagcca acatcatgaa
    18721 gttgaccgac ggcatgttca ttcggtgcgc acggcaaatt cacgacgacg aaggattcag
    18781 tgacatcgag tatgaagaag tgatcatcga tgcgggttgt atgaagctcg tgcaggaccc
    18841 gacgcaattc gacgtgcttc ttctcgagaa tctctatggc gacgtgctga gcgacctttg
    18901 cgcagggttc gtcggcgggc tcggtgtgat tccaggcgcg aacatcggca atgactacgc
    18961 ggtcttcgag gcggttcatg gatcggcgcc cgacatcgcg ggcaaggggc tggcgaatcc
    19021 cctcgctctc ctgatgtcgg cggtcatgat gctgaatcac atcgccgata cgcgtggcga
    19081 cgaaagctgc cggacctcgg caggccgaat ccgcgaggcc tacaaccgcg ctctatccga
    19141 cgggaaaaag acccgagacc tgggcggcaa tctcggcacc gaggatttcg cttccgcagt
    19201 gatcgagcgg atcgctggct agccgtcgtc ttcctggcct gcccggacac cgccgtcccc
    19261 acccgcaacc ctcagtagag gtggaaggac gatcaaagtc gcgaccaaac agccgccgat
    19321 ggtgatcgcg atcaattcgc cgaaattccg gaccgggacg aaggagctca ccagaaggac
    19381 ggagaagccg gccatcaagg tgacgcagga gatcacgatg gcacgtcccg tcccgcgggc
    19441 ggcgcgaacc agagccgctt cgcgaccgat ccctttgtgc atttcctctc gaaaacgggc
    19501 gagcacgtgg atcgttccgt tcaccgctag acccaagctg atagagaaaa tgatcacggt
    19561 cgagacgttc agcggaattt cgcgccagac catgtacgcc atggtcgcaa cgagcgggat
    19621 caagttgggc gggatgctca gaaggcccag ccggatgctt cggaagaaga aagccaagag
    19681 caggaagatc accagtaccg cgactagaag gcttccgagc aggtcgctca cgacggccgc
    19741 ttgcccgcgg gagcccgtgt atgcctcccc ggtgaacgag acggagaccg tttcatcgtc
    19801 tccaagctcc gactcgatca tcttttcgag cgtgtccagg aagcgcatcg tcgcctgggc
    19861 ccccacgtct cgcagcttga tttgaacccg agccttggtt ccgtcttccg tgagccattc
    19921 gccgagcggg cttgggttgc gctggctcat catcgtcacc aggccgcgga cttgcttggc
    19981 gctcttgaag ttttcggatc ggacgctcgg atcgtcggcc aacagagcgt acgactctcg
    20041 cagcatgtcg ccgtagctca tcgttcggat gacctcgggg cgcagagatg cccagtttcg
    20101 aatcctctgg atcttttgga tcatctcggg ttcggtgaag tgctctgggt ccgtgctcac
    20161 cagaatgact tccagaggcc tcacgcccgc gagcttttct tcgatgagga gggtcgtggt
    20221 gtagaccggg tcggtttcgt cgaactgatc gagcagagca tggtcgaccg tgatctgact
    20281 gccgacgtag cccgcaccga gcgtaaatac cgccgtgagc cccaagaccg cccagcgcca
    20341 cttcagcagc ttcgcgacga ggctgcccgt cacggtggtg aacatgctcg tgcgctctcg
    20401 gcgcacctgt tcgcgctttg ggggagcgac ccaggtgagg actgccggaa cgaaagtgat
    20461 gatgactagg tacgagagca tcacgccgat acctgaagtc aggccgaagt gtcgcagcat
    20521 cacggtcttg gaaacgacca gtgacgcgag tcccaccgcg gtcgtagccg acgtcagcat
    20581 gcaggctacc gcgatcgctc gcaccgttct gcgtccggca gcgatgcgat cactgtcttc
    20641 atcggcatgg acctcgtcgc gatagcgttg gatcaagtgc gtcgcgtcac ttatcccgat
    20701 gatgatcagc agcggcacga tgatgttgtt gaggatgttc atgggctcgc caaagagccc
    20761 catgccaccg acgaccacca atgccgtgat cgcaaccgcg cctagcggca gcaaaacccc
    20821 gggcacccaa cggaaggaca gcatcaggag ggccatgcag accaagaccg tgagtggaat
    20881 gagccgcaga ttgtcggctc tcatcttgct cacgatcacg ctgcgcagat acgggagtcc
    20941 accgacatga acgtcgatat tcgcgaactc gggccgctcc agtccttcgc gaagctcgtc
    21001 catggctcgc cgcatctgct tcgggtcgag gtcgtgcagc tgaagcgcga tgcccgcgac
    21061 cgtatgatct tcgtcgatca agcggccgac cagcagcggg ttgttcgcga ttgccgcacc
    21121 tagctcggca gcttcttctt cggtgacctc ggtacccttg acgatggggt cggtcttgag
    21181 ctcggcggac agggcagggc ctaccttcgc aatgccgtct gggaaacgac ccggatcgct
    21241 ctcgacgatg tcgagcagtg cattgaaggc ttcgtcttcg aggtcgccct gggcgtcccc
    21301 gacctcctcg gtgtcgtcca aggtgtcgag atcatccagg tcgctcagat catcgagatc
    21361 gccttctccg tccggctttc cgacctcttt aagcccatcg agattgtcga gatcgctctc
    21421 gtcagcgtcc ggcgctgcct tcactcgccg cgggatgttc agcgtagtga tcccggcgac
    21481 cttctccacc cagggctgat cggcgaagta gagggaaatg tcgtggatct gctgaagaga
    21541 ttcccgggag aggacatcgt cggcctcgac gagaatcaga acggcttcgc gtcgcttgcc
    21601 gaaccactgc tcgaagcgct tctgaatgag ggcgtattcc tcgccttcga acgaggcaag
    21661 gaggctttca ggggccggat ccgctgttaa tttggggatt tgggtcgtaa aagcaagcgt
    21721 gaccagaacg gacacccaaa ccacgagccg gcgccgctcg gtcacgagtc gggcaagcca
    21781 attgaggatt tgtcggcgtc gggacacgag cgtgtatgtc tgttactggc gggggtcgca
    21841 aggattctgt gaccggcttg acagccctcc tgggcgaggc taccacgcgc gtccctacgg
    21901 ggaggatatc agagtggcca accacgcctc agcaaaaaaa cgagtccgtc agacgatcaa
    21961 gcgcacagcg cgcaatcgta accttcgcac caacgtgcgc acctcggtga agcgcgtgcg
    22021 tgctgcaatc gaggcgggtg acgcaaatgc cgcgaagaca gctctcgtcg cggccgtgag
    22081 ccggatcgat ggcgccgttt ccaagggcat tttccatcgc aagacgggct cgcgttacat
    22141 cgcgagactg acccatcaag tcaacacgct tcagagctga gctctgctgt cgcgtcactg
    22201 agggtttgcg cagagctcga gcactgcgtc ttgaaggatc acgtccgggg gccgtttgct
    22261 gctcttgagc gcgcgatcgg ttcgcgcaat cagggtgaac gcctgcccga ggctgtccga
    22321 ggtgaaccgc ctcgccgact cggtgagatc cccggccttg aacggcgggg cgcctgcgcg
    22381 ctttgccgcg tcctgcggac gaaggccctc ggacagggcc tctctcatgc gggcgacgat
    22441 tcgcagctga cgcgccacca tcgcgagcag acggaggggc ggctcgcgat cgtcgagcaa
    22501 cgactgagct gcggcgatac ccttgcgacg gtccttaagt ccgatcgcat ccaccagggt
    22561 ccagatggtt tcgacccgaa tgcgcgtcac gcagcgcatg accgcgtcag cgtcgatgcg
    22621 ctgcccagca ccgacgaaca gcgatacgcg ttccattgca tcgtcgatag cggcgaggtc
    22681 gtcgcccacc gcatcgagca gcgcttcgat cgcttggggt gcaatggcgt gctcgcgcac
    22741 cgtggcttcc tcgcgaacga acttccgaag cgacgcgcct cgaagggagc tggcttcgat
    22801 gagatagccc ttcttcttgg cggttttcga aagcttggca cgtccatcga gcttgcttgc
    22861 agtggcgacg aaacaggtgg attcgcttgg atcgtccagg tattcagcca acttcgtctg
    22921 ctccgtgggt gtcatcgcgt ccacgttgcg caggagcacc aggcgcatgt cggccatcat
    22981 tgggagcgtg cgtgcagcgg atgttacgcg ccctgcttcg aggccctttc cgtcgaagat
    23041 ctcgacgttg aatccaggtg cgcccatcga atcgaccgca cgccggatgg cgtccacagt
    23101 gcgctcgatc agcaggcgct cggccccgac gaggacgtaa accggatcga gagagccctt
    23161 ggaggctctc ttggtgacat catggatgct tggcacggat cagcgagctt cgtcgtccga
    23221 gaaagtgact tcgccgctgt gttctactct gtctccgaga atttgaaagc ggtaacgtcc
    23281 gacctcttgc cggcgcaccg tgaccaccat ttcgattcgc cgattgggct cgaattgagg
    23341 gcgtttcaag cgcatcttgt gaacgatcgt gcgctgactt cgatcgttga cgaagaccgt
    23401 catcgttggc gcgatgaact tccgttccgc ggtctgaata tcgtagaaca ggacttcgaa
    23461 ttcggagtcg ccgaccggct ggttgaagtt cgcaaccaag gtggcgcgcc agtggcggtc
    23521 ctcgacgggt gcatcgttgc tttcgactag tcgtgtggct ttgttcgact gtgcaaaacg
    23581 gatcacgccg gattctgaga gcgagcctgg cacccctttt tgcgtcagga agatcttcgc
    23641 gcgaaggccc gaggcggcgt cggcaccctg ccacggggtt accgacgaga cgacggcggc
    23701 cagtgcgctt gcggccaatg cgaggcgtag attcacagct tgctcctggt tcgggatcca
    23761 ggatcaacct aacaactgga actttacagt gacagggtga taacgtgttc aaatgtaggt
    23821 tttaggcctt catgcttgat acccccggcc gaaaagttgt gccccagggc ggtgacagct
    23881 aactccgacc caggaacgga gctctatgac gacggtcagt gggggtgtga agggattggt
    23941 cgcccgcgcg cagcgcgaag cgcagaggcg cgcacagagc ccgtctacgg catatctcct
    24001 tctcgtgatg ctgcagacgg gaggccccat ggggaagctt ctgtccgagc ttggcgtgaa
    24061 ggagagcgac ctgctcagcg cgattcgcgt agtcgattct gagccgacga gcgcgctcga
    24121 gcttgcggtg gagcgcgcgt atcgtctcgc caagacgacg ggcgcgcgcc aggtgctgcc
    24181 cgcgcatctg ctcatggcca tcgctcgcga ctcccggagt gcggcgcacc gaagcctgga
    24241 ggtcatcggt gccggttcgc ggcgggtgca ggaagcggtt caagtcacgc tcgagccggc
    24301 agaggcacga agcgagccga gctctacgca gatccgaccg accgcctcat tcgcggcgca
    24361 gactcagtct ccggcgaagc ctttgcggcc ctccgcacgc cgatcggtgg acccggcgcc
    24421 gttgatcgag ctgcacgtcg aatcgcccga ggacgagatt gtcatcgagg caccgatccg
    24481 tggtccggcc aacgccttcg aagaccaaga cgagctggtc gtaccttcgt catcgccctt
    24541 catgttggat gcgggcaagt accctacgct cgcgaagctt gggcgcaatt tgacggagat
    24601 ggcatcgctc ggacgcatcg acgaggtcat cggtcgcgat gacgaaatcg agagacttct
    24661 cgacgttctg gcgcgcaggc gatcgaacaa tccgattctg gtggggccac ccggcgtggg
    24721 gaagaccgcc atcgtagaag gtctcgcgag gaggttggcg caaggcggcg atggcgtgcg
    24781 cggtctcgaa ggcaccatcc tggtcgagct ttccgcgggt ggtttggtgt ccggaacggg
    24841 agtgcggggc gcgctgtcag agcgcatgcg ccgacttcag ttcgaagtga agcgagcgaa
    24901 cggaaagatc attctcttca tcgacgaagt ccacacgctc ttcatcggag gcgattcgcc
    24961 ggacgatctg gcacacgagc tgaaggcatc ccttgcgagg ggtgagcttc cctgcatcgg
    25021 cgccacgact gacgtcgagt accgcaagta cgtggagcgg gacgccgcgc ttgcgcgtcg
    25081 gttctcaccg attcacgtgt cggaaccaag cgcggaagac gcgatcgaga ttcttcacgg
    25141 tattgctccc cggtacgagg tgcatcacgg agtggcgtac gagccatctg cagtcgagtg
    25201 tgcggtcgag ctctcggttc gctacatgtc ggagcaaaca ctcccggaca aggcgattgg
    25261 aattctagat ctcgctggcg cacgcgctcg ccgccggggg ggggcggtcg tcgatcgcga
    25321 agccatcgcg caggtcgttg ccgagaaggt gcgcgttcca gtcgaacgaa ttctggtgac
    25381 ggactcacag aagctgctcg agctcgagca gcatctcggg gagca
//
LOCUS       DASRZF010000006         6343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3097959, whole genome shotgun sequence.
ACCESSION   DASRZF010000006 DASRZF010000000
VERSION     DASRZF010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 6343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6343
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3097959"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(270..857)
                     /locus_tag="VFG22_00290"
     CDS             complement(270..857)
                     /locus_tag="VFG22_00290"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HET6414700.1"
                     /translation="MGDKKQRKIRNYLIDRRFQLKYTGMVLLVTVSVASGLGYVAYRF
                     SKGQTEALTAHIASQPDLDADTANDLEEFAQQEDRKIRNAIVFGVLLLTLALGITGII
                     VTHRVVGPAYRMKRLFAHVGSGRFEVTTGIRKGDELQELYHSFAEMVESLRDQRAGEI
                     EQLEETLRKMDEAGIQSEYITQLRAVLERIRKTVA"
     gene            complement(896..1663)
                     /locus_tag="VFG22_00295"
     CDS             complement(896..1663)
                     /locus_tag="VFG22_00295"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET6414701.1"
                     /translation="MFAGTAQADARQDYLLRLLRESDAFRVRAQAALSLGQLESTRKT
                     LTALEQAVSDRHPAVRISAISSLRHLGAKESLPMVEQATRDKNKAVRRAALEAAAALS
                     AIGERERGPGKTEYLVAIGMPGAKAERVSRQMLFDARIFLSEQLLSLQGIELAPDDLT
                     PADAKREIVQRRLDGYYLESSIVSLEPKGPGVRAVVSIIVLSYPGRDMRGMLRGAATA
                     SGHYGADTAEQAIQGAYVGALRRLPTVLQSAKGKGSL"
     gene            1870..3114
                     /gene="ftsY"
                     /locus_tag="VFG22_00300"
     CDS             1870..3114
                     /gene="ftsY"
                     /locus_tag="VFG22_00300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HET6414702.1"
                     /translation="MESLDLNNPTILGAVAAVAALIVIGIIIGLVRGRRRTQLPKAPP
                     PPPPEAFAEERPKIAAEPSGKAEPEPTQPIQLEDLVSRAPAPRAAPEPRPTPPPPPPT
                     DLSAFRSGLKSTRGNLISRLANVFRRGRELDASILDEVEEVLITADVGVHTTQRVLEA
                     LRSKMNGGQLSSADEAWEAIRAESLEILASSGGGLTVADAPTVILVVGVNGVGKTTTI
                     GKLASRLSRQGKSVLLAAGDTFRAAAVTQLEVWGRRVGCEVVKGKDRADPGSVIFDAI
                     QKAKDTGADVVIADTAGRLHTKTPLMDELKKLGRTVEKALGRPADQVLLVLDATTGQN
                     AIQQAQLFKEALPLTGIVLTKLDGTAKGGVVLGIVDQHHVPVQFVGVGEKVEDLKPFD
                     AEAFVQALFESPDAVEAAQDPG"
     gene            3282..4181
                     /locus_tag="VFG22_00305"
     CDS             3282..4181
                     /locus_tag="VFG22_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel domain-containing
                     protein"
                     /protein_id="HET6414703.1"
                     /translation="MRDFRGIALLMVALWMAIPTAAEAQMEFSLDEVEEDTKPAGKPK
                     DQGDLIEELAAADDAADGPAELTPQREEIAEEIYAVQQIYALRLKRLELAPSVAFTLN
                     DPYIKRYGLGFAMNYWFTNVLAIGINFNWYDWGGNPLERGTNLEFRVRNQFYLAAPIN
                     DWQFGLWANFTYVPFYGKFKVRKKIFQWDSYLIGGVGIMRTQPIPVFDPEIRNFDWQT
                     NVAFNVGAGLRIFLTRYLTFFTEFRAYMWPDQFENEQPVPPSDRDDQSMWLQSGSTFV
                     ANTSIQIGFTLFLPVKFEYRLPK"
     gene            4184..5068
                     /locus_tag="VFG22_00310"
     CDS             4184..5068
                     /locus_tag="VFG22_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414704.1"
                     /translation="MLARQTLLRIGLLAALALCLILGDGMRDGVGSASAQDVQVSGPL
                     AGAPAVRKLRLWREMRLHVEPFFAFTVGDEYSRSLIVGGEARFHFLDWLGIGGWGGYS
                     VAQLDTNLTQEIQAKGSTRPLNVLDLPSSENFPDQVGRLNWWSGVELHFVPFRGKLAM
                     FQKVFFDADLDFFVGAAFVGVEERADAVGQPAAGDVSFCEIAGNPDDQGCLESQLTRS
                     KRTAIAPSFGVSFNAFFQDFLGIALRWRGIPFKYNTGGTDNNNDGVINSSDRIKQFNN
                     MFTVGLIFVLPPKTKTTD"
     gene            complement(5081..5797)
                     /locus_tag="VFG22_00315"
     CDS             complement(5081..5797)
                     /locus_tag="VFG22_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET6414705.1"
                     /translation="MGLFDVFKGGGGGLKKHVDRVGNKRAQKHERWESIQVLANDGSD
                     EAIRALLTRFTIRVDPSITDGEEKNAAFHGVVQNGEAALEPVREFLSSSETLAWPLKI
                     LKELQTEEEVTTTLLDLLSKMDTEYERDPQKKIDLIASFEESRDPRIVEAVIRFLDDM
                     NETVRFHAAGAILAQDEADAAREAMTAAFIAEESVRVRMRMLDGYVERGWTLDDVKEE
                     ASKRMPTGYTLGKKGEVRKK"
     gene            complement(5868..>6343)
                     /locus_tag="VFG22_00320"
     CDS             complement(5868..>6343)
                     /locus_tag="VFG22_00320"
                     /inference="COORDINATES: protein motif:HMM:NF041621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MXAN_5187 C-terminal domain-containing protein"
                     /protein_id="HET6414706.1"
                     /translation="ILAVINGRGDVRLEVETPELGGLSYRVNQLINLFTGVAEEDDEG
                     RAVTSSGAWEAVSITGPDSALRQSSVGSSQSDDPEALALAALSEGEYYAQLFREYVAA
                     KQSVGEDVSSIPEERFVERMKGNAAHLAKKHGSRTVRFKVETIGGQVNLRPVVIR"
BASE COUNT         1206 a         2017 c         1884 g         1236 t
ORIGIN      
        1 gacgtacgac caggctcgac ccaatcgctg cgctgcccat cccgagccgt cctcctcggt
       61 atctgttgcc gtacggaggg gcgcggggga acgcgatgcg gggttccggc gacgttgcga
      121 gtgacaatcg gtcagggagc aaccaccccc gattggcgcg agcgtcgagg cgaggcttgc
      181 ggggtccaag cccccaagtg catccgacca caattgcaag ccgagccgtt cgacggaacc
      241 ccgtgtcacg ttccgctgcg gcccggtggt taagctaccg tcttgcggat tcgttctaga
      301 acggctcgga gctgagtgat gtattcggac tggatgcctg cttcgtccat cttgcggagc
      361 gtttcctcca gctgctcgat ttccccggct cgctggtcgc gaagggattc aaccatctcg
      421 gcaaaggaat gatagagctc ctgcagctcg tcgccttttc ggattccagt cgtgacttcg
      481 aaccttccgc ttcctacgtg cgcgaacagc cgcttcattc gataggccgg cccgaccacg
      541 cggtgagtga cgatgattcc ggtgatgccc aacgcgaggg tcagcaggag cacgccgaag
      601 acaattgcgt ttcgaatctt tcgatcctct tgctgcgcga actcttcgag atcattggct
      661 gtgtccgcat cgagatcggg ctgggacgcg atgtgcgcgg tgagagcctc ggtctgccct
      721 ttggagaagc gataggccac gtagccgagc ccgctggcga cggacacggt gacgagcagg
      781 accatgcctg tgtacttcag ctgaaaacgc cgatcgatca ggtagtttcg aatctttcgt
      841 tgtttcttat cgcccatcac gtttctcact tcctacgccc accgttctcc ggaggtcaaa
      901 ggcttccttt tcccttggcg gactgaagca ccgtggggag acgacgaaga gcgcccacgt
      961 aggcgccttg aatggcttgt tcggccgtgt ctgcgccgta gtgtcccgag gcggtggccg
     1021 cgccccgaag cattccgcgc atgtcgcgac ccggatacga gagaacgatg atcgaaacga
     1081 ctgctcgtac tccagggccc ttcggctcca agctcacaat cgagctctcg aggtagtatc
     1141 catcaagccg tcgttgcacg atctcgcgct tggcatcggc gggcgttaaa tcgtcaggcg
     1201 ccaactcgat gccttgaagc gacagcaact gctccgacag gaagatccga gcatcaaaca
     1261 gcatctgccg ggaaacccgc tcggccttgg cgccaggcat gccgattgca actagatatt
     1321 cggtcttgcc cgggccccgt tcgcgctctc cgatcgcgct gagagccgca gccgcctcga
     1381 gggccgcacg ccgcaccgcc ttgtttttgt cccgagtcgc ctgctccacc ataggcagcg
     1441 actccttggc tcccaggtga cggagcgagg aaatcgccga tatgcggacc gcggggtgcc
     1501 ggtcgctcac agcctgttcg agcgccgtta gggtcttacg tgtagattcc agctggccga
     1561 gcgaaagcgc agcttgagcc cggacgcgaa acgcgtcgga ctcacgaagc aagcgcaaga
     1621 gatagtcctg tcgcgcgtcc gcctgagcgg tgcctgcgaa catgagcaca accgcgaccg
     1681 aaagtcgcca ccagtgccat cggccgatgt gccgcctcaa caactcccgc ccttcccctg
     1741 cgttttcagt atcacagctc gcgaggcggg tcggcaaacg attcggtccc gccgccgcgt
     1801 tgactgccca gtgctcagat ccctatattg cggcggtccc aacgaccatc gggggatgca
     1861 agaggtcgaa tggagtctct agatttaaac aacccaacca tccttggggc ggtcgctgcc
     1921 gtcgccgcac tcatcgtcat cggcatcatc atcggattgg tccgaggccg acgaaggacg
     1981 cagcttccga aggcaccgcc cccgccaccg cctgaagcat tcgccgagga acggccgaag
     2041 atcgcggccg agccatcggg caaagcggag cccgagccga cccagcccat tcagctggaa
     2101 gacctcgtat ctcgggcgcc cgcgccccgg gcggcgccgg agccgagacc gacgcctcca
     2161 cctcctccgc cgacagacct cagcgcgttt cgtagcgggc tgaagtccac gcgtggcaac
     2221 ttgatttcac gcctcgccaa tgtcttcagg cggggccgag agctcgatgc atcgatcctc
     2281 gatgaggtcg aagaagtcct gatcacggcg gacgtcggcg tgcacacgac tcagcgggta
     2341 ctcgaagcac tgcgcagcaa gatgaacggc ggccagctct cctctgcgga tgaagcatgg
     2401 gaggcgatcc gtgcagagtc gcttgaaatc ctcgcgtcct caggtggcgg gctcacggtt
     2461 gcggacgcac ccacggtgat cttggtagtc ggtgtgaacg gcgtcggtaa gacgaccacc
     2521 atcgggaagc tggcaagtcg tctgagtcgt cagggcaaat ccgtgctctt ggccgcgggc
     2581 gatacgtttc gcgccgccgc ggtgactcag ctcgaagtct ggggcagacg ggtcggatgc
     2641 gaagtcgtca aaggcaagga tcgcgccgat cctggctccg tgatcttcga tgcgattcag
     2701 aaagcaaagg acaccggagc cgacgtagtg atcgcagaca ccgcggggcg ccttcacacc
     2761 aagaccccgc tcatggatga gctcaagaag ctcgggcgca cggtggaaaa ggcactcggc
     2821 cgtcccgccg atcaggtgct gctcgtgctc gacgccacca ccggccagaa cgcgatccag
     2881 caagcccagc tcttcaagga agcccttccg ctcactggga tcgtcttgac caagctcgac
     2941 ggaacggcca agggcggtgt ggtcctgggc atcgtcgacc agcatcatgt gcccgttcag
     3001 ttcgttggag ttggagaaaa ggtcgaggat ttgaagccat tcgacgcaga ggcgttcgtg
     3061 caggcccttt tcgagtctcc agacgcagtc gaggcggcac aagacccggg ctagcagccc
     3121 gcggctgccc ccgaatcagt caaaaatcgc gaccagattt ggtagtgcga tattcgccat
     3181 ggtatagtct tgtggtccga gtgaaatcgg acagttgcta cgtgatttca gagagttgcc
     3241 acggtggggg ccggggcgct cggggggaag gccaaaggcc gatgcgagat tttcggggga
     3301 ttgcgctctt gatggtggcg ctatggatgg cgattccgac cgcggccgag gcccagatgg
     3361 agttctccct cgacgaggtc gaggaggaca cgaagcctgc cggaaagccg aaggaccagg
     3421 gcgacctcat cgaagagctg gccgcggccg acgacgccgc agacggtcca gcggagctca
     3481 cgccgcagcg tgaggagatc gccgaggaga tctatgcggt ccagcagatc tacgcgcttc
     3541 gcctgaagcg cttggagctc gcgccttccg tcgcgttcac actgaatgat ccgtacatca
     3601 agcgctacgg actcggcttt gcgatgaact actggttcac caacgtcttg gcgatcggta
     3661 tcaatttcaa ctggtacgac tggggaggaa acccactcga acgtggaacc aacctcgaat
     3721 tcagggtgcg caaccaattc tacctcgctg cgccaatcaa cgattggcag ttcgggctct
     3781 gggcaaactt cacctacgtt cccttctatg ggaagttcaa ggtccgcaag aagatctttc
     3841 aatgggactc atatctcatc ggcggcgtcg ggatcatgcg gacccagccg attccggtgt
     3901 tcgatcccga gattcgcaac ttcgattggc agaccaacgt cgcattcaac gtcggcgcag
     3961 gtcttcgtat cttcctcact cgctatctga cgttcttcac cgagttccga gcctacatgt
     4021 ggccggacca atttgagaat gagcagcccg ttccgccctc agaccgagac gaccaatcga
     4081 tgtggctcca gtccggatcg accttcgtcg ccaacacctc gattcagatt ggattcaccc
     4141 ttttccttcc ggtcaagttc gaataccggc taccgaaatg atgatgctcg cacgacagac
     4201 gcttttgcga atcggcttgc tggcagcgtt ggcgctttgc ctgatcctcg gagacggcat
     4261 gcgtgatggc gttggctcag cgagcgcaca agacgtacag gtgagcgggc cgctggctgg
     4321 cgctccggcc gtgaggaagc tgcgcctctg gcgtgagatg cgcctccacg tcgagccttt
     4381 ctttgcgttc acggttggtg acgagtactc gcgatccctc atcgtcggcg gcgaggcccg
     4441 gttccacttc ctcgattggc tcggaatcgg cggctggggc ggctactccg tcgctcagtt
     4501 ggatacgaac ctcacccagg agatccaggc gaagggctcc acccgcccac tgaacgtctt
     4561 ggatctgcca agcagtgaga acttccccga tcaggtcggg cgcttgaatt ggtggtccgg
     4621 cgtcgagctg cacttcgtgc ccttccgagg aaagctcgcg atgttccaga aggtcttctt
     4681 cgatgctgat ctggacttct tcgtcggtgc ggccttcgtc ggcgtggaag aacgcgccga
     4741 cgccgtcgga caacccgcag cgggagacgt ctctttctgc gaaatcgcgg gaaatccaga
     4801 cgatcaaggc tgccttgaga gccagctcac tcgctcgaag cgcacggcta tcgccccttc
     4861 attcggcgtc tcgttcaacg ccttcttcca agactttctc ggcatcgccc tgcgatggcg
     4921 cggcattccg ttcaagtaca acacgggcgg caccgacaac aacaacgacg gtgtgatcaa
     4981 cagcagcgac cgcatcaagc agttcaacaa catgttcacg gtcggtctga tcttcgtcct
     5041 tccgccaaag acgaagacca cggactaggc cagcaggcgg ctacttcttt cggacttcgc
     5101 ccttcttgcc gagcgtgtac ccggtgggca tgcgcttgct cgcctcttcc ttgacgtcat
     5161 ccagcgtcca gccgcgctcg acgtagccgt cgagcatgcg cattcgaact cgaacgctct
     5221 cttcggcgat aaaagccgcc gtcattgcct cacgcgccgc gtccgcttcg tcttgggcga
     5281 ggatggcgcc cgctgcatga aaccgaaccg tttcgttcat gtcgtctaga aagcgaatca
     5341 cggcttcgac gatgcgcgga tccctggatt cctcgaagct ggcgatcagg tcgatcttct
     5401 tttgcggatc tcgttcgtac tcggtgtcca tcttggagag aagatcgagg agagtggtgg
     5461 tgacttcctc ttccgtctgg agctccttga gaatcttcaa gggccaagcc aaggtctccg
     5521 acgaggacag gaactcgcgc acgggctcga gcgcagcctc accgttctgc accacgccat
     5581 gaaaagctgc gttcttctcc tcgccatcgg tgatgctggg gtcgacacga atcgtgaacc
     5641 gggtcagcag cgcccggata gcctcgtccg agccgtcgtt cgcgagcacc tgaatcgact
     5701 cccagcgctc gtgcttctgg gcacgcttgt tcccaacccg gtccacatgc ttcttcagcc
     5761 cgccgccgcc acccttgaac acatcgaaaa gccccatgac ggctcaagca ctaggcccaa
     5821 cggggcgccg gcgcaacgag atgctgcttg ccacttggag cgcgccgcta acggatgacc
     5881 accggcctca gattcacctg gcccccgatg gtctccacct tgaagcgcac cgtccgcgag
     5941 ccgtgctttt tggcgaggtg tgcggcattg cccttcatcc gctcgacgaa acgctcttcg
     6001 ggaatgctcg aaacgtcctc gcccacagat tgcttggcgg cgacgtactc acggaagagc
     6061 tgcgcgtagt actcgccctc ggaaagcgcg gcgagcgcca gcgcctccgg gtcatcgctt
     6121 tgactcgagc ccacggagct ctgccgcaac gccgaatcgg gaccggtgat gctaaccgct
     6181 tcccaggcgc cgctgctcgt caccgcccgc ccctcgtcat cctcctcggc gacaccggtg
     6241 aacaaattga tcagctgatt cacccgatac gagaggcctc cgagctccgg agtctccacc
     6301 tccaggcgaa catcgccacg tccgttgatt acggcgagga tgt
//
LOCUS       DASRZF010000007         4107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3098476, whole genome shotgun sequence.
ACCESSION   DASRZF010000007 DASRZF010000000
VERSION     DASRZF010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3098476"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..1428)
                     /locus_tag="VFG22_00325"
     CDS             complement(136..1428)
                     /locus_tag="VFG22_00325"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6414707.1"
                     /translation="MSTEARVSPYRWVVLFVFMLITMVIQLQWLAHAAVARPAAVFYD
                     GQFDPSSFINIDFLAMVYMLVFLVMSFPASYVIDTYGIRTGISLGAGLAGVCGLTKGF
                     FADDFQTVVVSQIGLAVAQPFVLNAVTAMSVRWFPLRERGTAAGLSALAQYLGILVAM
                     LATPFLVGSDPSKPGYGTGFEGMLLAYGWLTFGAAVLTLLLLKDRPATPPEVETKEKQ
                     SFGKGILHILRLRDMQITLYLFLVGLGIFNAVSSMTDAIAASAGVQDSDGLIGGLMLV
                     GGILGASILPVLSDKFRKRKPFLVACVVGMVPGILGLSFAGYLSADPGTVYAISLVSS
                     FVLGFFIMSAGPLGFQYAAEVSHPAPESTSQGMLLWVGQITGMIFVAGMSIESKRYLP
                     AFMLAFSILAIISAFVVSFLKESPMIITEQDRVADRPS"
     gene            complement(1425..2744)
                     /locus_tag="VFG22_00330"
     CDS             complement(1425..2744)
                     /locus_tag="VFG22_00330"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6414708.1"
                     /translation="MTEHSTLGRVLKRKPVVAWALYDWANSAFATTVMAGFFPVFYSA
                     ITRDLSTEDSQFWFNVTLAVASILIAASAPILGAVADRGGARKKFLVFFASIGILMTG
                     GLAWVHAGHWWMGLLLYGLGTVGFSGANVFYDSMLVDVAEDSELDLVSALGYSMGYIG
                     GGLLFGLNVLMVQKPEWFGLESAGSAVKASFLSVSVWWALFTIPLLRFVEETPTQYKA
                     APLEALGAGLRQLLATLKEIQSFKVLMLFLVAYWVYIDGVNTVIKTAVFFGDRVLGLE
                     QSALITALLLTQFVAFPAALAFGALGQRIGPRPAILIGIAVYLAALVYAWRFLQDEGD
                     FYVLAVAIGLVQGGVQSLSRSLYARMVPSSKAAEFFGFFNMVGKFATIFGPLLIAFTP
                     KLIPGATDRDGILSLAILFLVGGFLLSRVRVAEGIEAAQRFDQDGTP"
     gene            complement(2851..3264)
                     /locus_tag="VFG22_00335"
     CDS             complement(2851..3264)
                     /locus_tag="VFG22_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414709.1"
                     /translation="MTAVIWFVQLVQYPSFAKVGAAGFADFHAHHSARITLIVAPLMI
                     AEAVSAIALLWRPPSGIVQWEIWVGLGLLLIIWASTFLLQVPMHTKLSSAFDADAWRF
                     LVRSNWIRTLAWSLRAVLVTVWLHRVLAQASRLGA"
     gene            3437..3994
                     /locus_tag="VFG22_00340"
     CDS             3437..3994
                     /locus_tag="VFG22_00340"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HET6414710.1"
                     /translation="MKLEISASQRPSHDTSHDRLSVLRDAHRTLIALADGADSSPGAQ
                     TAAARTVNELAKCFRSGRLPEDQAEWVMVLEAIDHVVLGDPDATETSALVMLVQQGTV
                     TGAFVGESLAYIIPPVGEAQLLTPGGRRAPGIGTGFASPRGFGPVKLDGKLITRRAAR
                     FEPDDLPAAIASRRESAASASSALW"
BASE COUNT          934 a         1208 c         1288 g          677 t
ORIGIN      
        1 ttcggcacat ccacagtagc gaaggtttcg gcatcgatcc tgccgatgtg cacgaacgct
       61 tcgcgggcta ctgcgagcgc ttcgacctgc tggcctaact gccccgcttc atgtccgggc
      121 gaagtttctg ggcgatcaag atggccggtc ggcgactcgg tcttgctccg tgatgatcat
      181 cggagactcc ttcaaaaaag agacgacgaa cgcagaaatg atcgcgagaa tggagaacgc
      241 aagcatgaac gccggcaggt accgtttgct ctcgatgctc atgcccgcga cgaagatcat
      301 gcccgtaatc tggcctaccc agagcagcat gccttgcgag gtcgactccg gagcggggtg
      361 actgacttcg gccgcgtact gaaagccgag cggaccggcg ctcatgatga agaagcctag
      421 aacgaacgag gagacaagac tgatcgcgta aacggtgccg ggatcggctg agaggtatcc
      481 cgcgaacgag agcccgagga ttcccggcac cattccaacg acgcacgcga cgaggaatgg
      541 ctttcgcttc ctaaatttgt cggaaagcac cggcaagatc gacgcaccga ggattccacc
      601 gaccagcatc agaccgccaa tgagcccgtc cgaatcctgg acgccagcgc tggctgcgat
      661 tgcatcggtc atcgaactca ccgcgttgaa gatgccgagg ccgaccagaa acaagtagag
      721 cgtgatctgc atgtcccgca gtctcagaat gtgcaggatg cctttgccga acgactgctt
      781 ctctttggtc tcgacctcgg gcggtgtcgc aggtcgatcc ttcagcaaga gcaaggtcag
      841 gaccgcggcg ccgaaagtaa gccatccgta cgccaagagc atcccttcga atcccgttcc
      901 gtatcctggc ttgctggggt cggagccgac gaggaacggg gtcgcaagca tggctacgag
      961 gatgccgaga tactgcgcga gagctgaaag gccggcggcc gtgcctcttt cccgaagcgg
     1021 aaaccaacga acggacatcg ctgtgaccgc attgagtacg aacggctgcg ccactgcaag
     1081 cccgatctgc gagacgacga cggtttggaa gtcgtccgcg aagaagccct tcgtcaaacc
     1141 gcagacgccc gcgagaccgg cgccaagcga gatcccggtt cgaatcccgt atgtatcgat
     1201 cacgtaggat gcggggaagc tcatcaccag gaagacgagc atgtagacca ttgcgaggaa
     1261 gtcgatgttg atgaacgacg agggatcgaa ctgtccgtcg tagaacaccg cagcgggtct
     1321 cgcgacggcg gcgtgcgcga gccattgaag ctggatgacc atcgtgatca gcatgaacac
     1381 gaataggacg acccagcggt agggagagac tcgggcttcg gtgctcacgg cgtgccgtct
     1441 tggtcgaagc gctgagcggc ctcgatgcct tccgcgaccc gaactcggct cagaaggaag
     1501 ccgcctacga ggaagaggat ggcaagcgag aggatgccgt cacgatcggt agcgccgggg
     1561 atcaatttgg gtgtgaacgc gatcaagagc ggtccgaaga tcgtggcaaa ttttcccacc
     1621 atgttgaaga atccgaagaa ctccgccgcc ttcgacgacg gaaccatgcg agcgtagagc
     1681 gagcgtgaaa ggctttggac gccaccttgc acgaggccga tcgcgaccgc gagtacgtag
     1741 aagtcgccct cgtcttggag gaagcgccag gcatagacca aagccgcgag atagaccgcg
     1801 atgccgatga ggatggcggg acgcggacct attcgctgac ctaaggctcc aaaggccagc
     1861 gcagccggga aggcaacgaa ctgggtgagg agcaaagccg tgatcagcgc cgattgctcg
     1921 agaccgagaa cgcggtcgcc gaagaacacc gccgtcttga tcacggtgtt cactccatcg
     1981 atgtagaccc agtacgcgac caggaagagc atcaggacct taaagctctg gatctccttg
     2041 agggtggcca gcaattgccg gaggcccgca ccgagcgctt cgagtggcgc ggccttgtat
     2101 tgcgtaggag tttcttcgac gaagcgcaga agcgggatgg tgaagagcgc ccaccaaacc
     2161 gaaacgctca aaaaggaagc tttgacggcg ctgcctgcgc tctccagccc gaaccattcc
     2221 ggtttttgca ccatcagcac gttgagtccg aacaagaggc cgccgccgat gtagcccatc
     2281 gagtagccga gcgccgaaac cagatctagc tctgagtcct cggcgacgtc gacgagcatc
     2341 gagtcgtaga agacgttggc gcccgagaag ccgaccgtac cgagtccgta gagcagcaaa
     2401 cccatccacc aatggcccgc atgcacccag gcaagcccgc cggtcatgag aatgccgatg
     2461 ctcgcgaaga agacgagaaa cttcttccgg gcacctcccc gatcggcgac ggctccgaga
     2521 atcggcgcag atgcggcgat caaaatactg gccacggcga gcgtgacgtt gaaccagaac
     2581 tgagagtctt cggtgcttag atcccgagtg atcgcgctgt agaagacggg aaagaagccg
     2641 gccatcacgg tcgtggcaaa cgcggagttc gcccaatcgt agagcgccca tgcgacgacg
     2701 ggcttgcgct tgaggacgcg ccctagagtg gagtgttcgg tcatcttccg agctccaaac
     2761 gagactagcg accgcgaagg actgtgtccc cttttcggac gaagatcgac gccatggcga
     2821 aggtcctgtc aggcgctcgg cctgcgcagc tcaagcgcca aggcgggaag cttgcgcgag
     2881 aacgcggtgg agccagacgg tgacgagaac cgcccggagc gaccaggcca gcgttcgaat
     2941 ccaattgctg cgcacgagaa accgccacgc gtctgcatcg aaagcagagc tcagcttcgt
     3001 gtgcatcggg acctgcagaa gaaacgtgga cgcccagatg atcagcaaga gacctagtcc
     3061 cacccagatc tcccattgca cgatgccgct cggcggtcgc cagagaagcg cgatcgcgga
     3121 aacggcttcg gcgatcatca gaggcgcgac gatcaaggtg atacgcgcgg aatggtgcgc
     3181 gtgaaaatcc gcaaagcctg cggcaccgac cttggcaaac gatggatact gaaccagttg
     3241 cacaaaccag atcaccgctg tcatcgccca agttgtaatg atttgtatca gtagggggat
     3301 catgagcgtc tcgattggcg cgtcatcagt cgtgccgcgg atttgccaat atggcaacga
     3361 ctgcgtgcca atggattttg gaatagtgcg taacgcgcgg tggttaggca cagtaggcat
     3421 agcaacgggg gcattcatga aactcgagat cagcgcgtcg caacgtccaa gtcacgacac
     3481 gagccacgac cggctctcgg tgctgcgcga tgcacatcga accctcatcg ccttggcaga
     3541 cggcgcagat tcgagtcccg gtgcacagac cgcggccgcg cgcactgtga acgagctcgc
     3601 aaagtgcttc cggagcggca ggcttcccga agatcaggct gagtgggtca tggtgctgga
     3661 agcgatcgac catgtcgtgc ttggcgaccc cgatgccacc gaaacgagcg cactcgtgat
     3721 gctggtacag cagggaacgg tcacgggtgc attcgtcggg gagagcctcg cctacatcat
     3781 cccgcccgtt ggggaggccc agctgctcac gcccggggga cggcgagcac cggggatcgg
     3841 gaccggtttc gcgagccccc gtggcttcgg gcccgtgaag ctcgatggca agctcatcac
     3901 gcgcagggcc gctcgattcg agccggacga cctgccggcc gccatcgcca gtcgtcgcga
     3961 atccgcagcc agcgcatcat cggccctctg gtagtcgccg ccgttggtgg ccgccgcgtc
     4021 ggcgcccgga gtctgaatcg ggttgccaag gttagtttac aagtcgtata gtaagagaat
     4081 gcaccaagtt acggagcttc cttacga
//
LOCUS       DASRZF010000008         8694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4475003, whole genome shotgun sequence.
ACCESSION   DASRZF010000008 DASRZF010000000
VERSION     DASRZF010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 8694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8694
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4475003"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1213)
                     /locus_tag="VFG22_00345"
     CDS             complement(<1..1213)
                     /locus_tag="VFG22_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414711.1"
                     /translation="MAAPLESDAKPRPTSARGAATQPEVLAVPVPFWKDGRAEGEVDA
                     AKADADGHLLVDLGEDWTPYILTESSSPDEEPLPNQYRETYLALARGEFPDDRHGYRA
                     EKDEYLELYGILPTLSLLRQRFAEVRALDCAEALDLEPLEQFEGFLAYEKGRRPARRL
                     ARYQLLKPKMAALVKRAGVENLDALTRAQVPDDDTWERVEEYRTIAPEIEAIIAAQAR
                     LKCEGFFEGRGVYTPGLFDWRTHEALAEFERRHRVYGWGFIGNDTLAVLQTTPEATEQ
                     EAVIRMLTERAMHAAEVIEDGSTSTLGDGEPRTYKTEDGRTLPIPNFEAELRERVITA
                     FGLQTPESTYAWLESLGEIPAKEIVAVRGIELPPYYGDNMDLSVLIDRGDVWYEFPFD
                     EEGKARSQPVNR"
     gene            1387..2793
                     /locus_tag="VFG22_00350"
     CDS             1387..2793
                     /locus_tag="VFG22_00350"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HET6414712.1"
                     /translation="MGVFERRTVGLVLTPALFLLAWFAPFNLEPSAQRLAAVFAAVMV
                     AWVTEVLPISVTALLIAPAMVVVGVTDSRSAFAPYADPLIFLFIGGFFIARAMMRHGL
                     DRRIARSLMQFRLVRGSPVRMRMAFMLTAVLLSMWISNTATAAILIPILLGTLPEDDE
                     RSSGSVLAIAYAASVGGMGTLIGSPPNFITVRFLQEQTNVQFDFVQWMGVGAPVALVL
                     AVMIGFVLQWLAPPPPTESAVTLVASPWSRGEKVTALCFGLAVIGWTVPGIMRAAGAP
                     YAEEVATALPIGAVAILAAAPLFLILDGSGKTPVLPWHDAVKIDWGLILLFGGGLSLG
                     AQMFQTGLAAAISQWFLGVTGISSLWGLTAALIVFTVFFTEACSNTASSNMIVPLAIA
                     AAVELGVSPLPPALAVGLAASCAFMLPIATGPNAVAYGTGLLDLPQMMRIGFVLNIVT
                     ALSLLAVLYALSMLYGWT"
     gene            2866..5154
                     /locus_tag="VFG22_00355"
     CDS             2866..5154
                     /locus_tag="VFG22_00355"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET6414713.1"
                     /translation="MVTMGTVPFLLNQLRPPGKGGAALTQAAASIAFAQGQPQTIVDV
                     LHGRAFRYYAEGLRQYLAIRTGSTERAEQCLSKLRAYVAKTETDELIQAPGVRARLYR
                     AARGYMHNQSRPHEPPSRSALEELPWRALPSAHLSVSPLNALRFDLSAEDSELLELLH
                     ARELSIEELAFVYGKTATEMEERVDAAMAQAQIILDEHGIRDAERFGRVIIEAFALEA
                     APRARSDAEEGIEPLPNGTIVGGRYAIEARVGLGAFGDVYRAKDTEVPGHVVALKLLH
                     QAAHSKSAKQSSLRELRLIASVFHPSIVQFKDHGWYEERLWFVMPWYDGETLESRLQR
                     EPLTRAEALAIFQPLARALAAMHAAGVRHQDVKPENIFLARIPGFGEDEVTPVLLDLG
                     VAATEAEMVVAGTPTYFAPEVAAQFASVEDKPSVNNKADVFALALSLRNALDPATQED
                     VAAGAVDTFIEYRSRETPKPPTLKALKYLTPFFARWLSRDPDQRPSADEFATELSALA
                     LPEERRERVKTILRWTIPMALALLGASGAAVTVFYQKAETQREEAHQAKLTALDLRRD
                     LADTKEIAEQLELRYEQSRLTRTDLARRLARTDGEVVSLKTQLKDEERRLMRTREKLT
                     QTRQEKANVDQSLSETERELAATERTLSATNQRLATTSQKLSAEEDRVRRLERKLERE
                     GERVIELYAQIAALDARLTAEASKTDELQDAVARAVAARSRAELELEFAQDEIKALRN
                     RPTPHAPAPEEEPPTKQSSPDS"
     gene            complement(5157..5333)
                     /locus_tag="VFG22_00360"
     CDS             complement(5157..5333)
                     /locus_tag="VFG22_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414714.1"
                     /translation="MPECRSCKDEVDELVSVKAAGRLIKVCEDCAERLAEEAEIAELG
                     ESAMQDMMGYKGRR"
     gene            complement(5338..6024)
                     /locus_tag="VFG22_00365"
     CDS             complement(5338..6024)
                     /locus_tag="VFG22_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414715.1"
                     /translation="MPTPQETVDAVRSYLRTHPEELGRAVRSALGLRFGVPLVALRWL
                     GSQAERSGKVEDFQIDSVPPGLRLSGNLDLMNTPIRAGAVIYVERVVFNDEELTVALR
                     LEDVMLKLNGESESPIAALIKSGALDLSNPGTLVNFMPGRSPVLAEANGNRIVLDMMR
                     DPKIGKNPLVRRAVSLLTSFVTLHGVETDKKHLDIAFRALPGGLGGAARSVRQNVVLP
                     SMSRFLPRAR"
     gene            complement(6066..6683)
                     /locus_tag="VFG22_00370"
     CDS             complement(6066..6683)
                     /locus_tag="VFG22_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414716.1"
                     /translation="MSARVPMFCLALASASFAACSGPTNDGYPCYESSECRGGSVCAE
                     TVYGNYCLETCSVETVFCENGQSCLQTEPLSDGAGGSGGDAGAGGMGGVGGMGGVGGE
                     GGVGGGAEEPLWVCMPGDVQDPDFVPRIIGQICSYSIECEVGAVCVCIPGATCEGEGK
                     NGPTCQRLCNSAVINQCPQVLDIQPECTDLGDNRGFCDPTTLVNN"
     gene            complement(6732..>8694)
                     /locus_tag="VFG22_00375"
     CDS             complement(6732..>8694)
                     /locus_tag="VFG22_00375"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET6414717.1"
                     /translation="GNQAMSFTWPTVASWVRWREGLGPRPSVLDAPPVLQEPEEADFD
                     EVDFDVELFYRTIVGALGNWWQRIGRSATDFGEQLDSLRWQLPRLSVLRQMRPDTRVS
                     LGRALAEKATSNPDGTFFLWEGRAFSYAQADRRVNHVVRGLIRSGVRRGEPVAVLMGP
                     RPSYLSVAAALSRLGAVPILLSPDRPRETLEEAIHASAPRFLIADPESAALGKQLFDE
                     VLVLGGAREERKLPPSVVDMELIDPGAVELPAWYEPNPGCARDLALIILTAGRGKKPR
                     AAKITNQRWAFSAYGTAAACTLSPSDTVYCCLPLHHPAGMLVAVGGGLVGGSRLALAT
                     RFEPTTFLDDVRRYGATVVFYAGEMLRELVRTEPSVADNENPIRLFAGSGLRRDVWRQ
                     VVDRFGPVGILEFYAATEGNAVLANASGEKVGALGRPLPGSAEVELVRYDFEDKEFVR
                     GEHGLLVRCEDDEPGILLARLDAEHPLASFTGGDEAGRRLLQGVFEPKDTWFITWDVL
                     RRDADGDYWFVDRASRMLRTGAGVVATRSIEDALYGFEALRQTVVFGVEEDGADRAVA
                     VVATVHDRGLDLEAWNAFSEGLRPSERPAWLKRVESIPMTDGFRPDKSALESDPLDAG
                     AELFVYDQKAQRYVVWQGNASSLPRAGML"
BASE COUNT         1604 a         2860 c         2669 g         1561 t
ORIGIN      
        1 gccggttgac gggctgcgag cgcgccttgc cctcttcgtc gaacgggaac tcgtaccaaa
       61 cgtcgccccg gtcgatcagg acgctgaggt ccatgttatc gccgtaatag ggcggaagct
      121 cgataccccg aaccgcaacg atttccttgg cggggatctc gccgagagac tcgagccaag
      181 cgtaggtcga ctcgggcgtc tggagcccaa acgccgtgat cacacgctcg cgaagctcgg
      241 cctcgaagtt gggaatcgga agcgttcggc cgtcctcggt cttgtaagtg cgtggctcgc
      301 cgtcgcccaa ggtcgacgtg ctgccatctt cgatgacctc tgccgcgtgc atcgcgcgct
      361 ccgtgagcat gcgaatgacc gcttcctgct cagtggcctc aggcgtcgtc tgcagcaccg
      421 cgagggtgtc attgccgatg aagccccaac cgtagactcg atgccgtcgc tcgaactcgg
      481 cgagcgcttc gtgagtgcgc cagtcgaaca ggcctggtgt gtacacgcca cgaccttcga
      541 agaacccttc gcacttcagt cgcgcctgcg ctgcgatgat cgcttcgatt tccggcgcaa
      601 tcgttcggta ctcttccacc cgctcccagg tgtcatcgtc gggaacctgc gctcgggtga
      661 gcgcgtcgag gttctcgacg ccggcgcgct tgacgagagc cgccatcttt ggctttagca
      721 gctggtagcg ggccaaacgg cgagcagggc ggcgcccctt ttcgtaggcg agaaaccctt
      781 cgaactgctc caagggctcg aggtcgaggg cctccgcgca gtcgagcgct cggacttccg
      841 cgaagcgctg cctcagaaga ctgagcgtcg gcagaatccc atagagctcg aggtattcgt
      901 ccttttcggc ccgataccca tgccggtcgt cggggaactc accacgagcc agtgccaagt
      961 acgtctcgcg atactggttg ggcagcggct cctcgtccgg ggaggaagac tcggtgagaa
     1021 tgtaaggcgt ccagtcctca ccgagatcga cgagcaggtg gccgtcggca tccgccttcg
     1081 ccgcgtccac ctcgccctcg gctcgcccgt ccttccagaa cggcacgggc accgcgagaa
     1141 cttcaggctg ggtcgccgcc ccgcgggcgg aggtaggcct cggcttggca tcgctctcga
     1201 gtggagcggc cacaggatcc tgctttggtg tacagccaca gaagaccgca cacgccagaa
     1261 ttgtcagtaa tcccagacgc aagtgagcca cgacccgctc accttggggc tgaacgcgat
     1321 gtagcgcaag tttcgggcgg accgggctgg acgcgcccat ggggggacgt gctagatggc
     1381 cggtggatgg gcgtttttga gcggcgcacg gtaggtctcg tccttacccc ggcactgttt
     1441 ctgctggcct ggtttgcgcc gttcaacctc gaaccgagcg cccagcggct ggcggccgtg
     1501 tttgcggcag tcatggtcgc ttgggtcact gaagtgcttc cgatttcggt gaccgcgctt
     1561 ctcatcgcgc cggcgatggt cgtagtcggc gtgactgaca gtcgttcggc ctttgcgcct
     1621 tatgccgacc cgctgatctt cctcttcatc ggcggcttct tcattgcccg ggccatgatg
     1681 cggcacggcc tcgaccgacg gatcgcccgt tctctcatgc agtttcgact cgttcggggc
     1741 tcgcccgttc gcatgcggat ggccttcatg ctgacggcgg tgctgctgtc gatgtggatc
     1801 agcaacaccg cgacggccgc catcctgatc cccattcttc tcggcactct ccccgaagac
     1861 gacgagagga gctccgggag tgtcctcgcc atcgcgtacg ccgcctccgt gggaggcatg
     1921 ggcacgctca tcggcagtcc tccgaacttc atcacagtgc gtttccttca ggaacaaacc
     1981 aacgtgcagt tcgacttcgt ccagtggatg ggcgtcgggg cgccggtcgc gctcgtcctg
     2041 gcggtcatga tcgggttcgt actgcagtgg cttgcaccgc ctccacccac cgagagcgcc
     2101 gtgacgctcg ttgcctcgcc ctggtcgcgc ggcgaaaagg tcaccgcttt gtgcttcggt
     2161 ttggcggtca tcggctggac cgtgcccggc atcatgcgag ctgcgggcgc gccttacgct
     2221 gaagaggtcg ctaccgcgct tcccatcggc gcagtggcga ttctagcggc agcaccgctt
     2281 tttctgattc tcgacgggag cggcaagacc cctgtgctgc cctggcacga tgccgtgaag
     2341 atcgactggg ggctcattct actcttcggt ggagggctct cgctcggagc tcagatgttc
     2401 cagaccggcc ttgcagcggc gatcagccaa tggttcttgg gcgtcaccgg aatcagctct
     2461 ttgtgggggc tcaccgccgc gttgatcgta ttcaccgtct tcttcaccga agcctgttcg
     2521 aacacggcgt cgtccaacat gatcgtccca ctcgcaatcg ccgcagcggt ggagctcggc
     2581 gtctctcccc ttccccctgc gctcgcggtc ggcctcgcgg cgagttgcgc cttcatgctc
     2641 ccgatcgcga cggggcccaa cgcagtggcg tatggaacgg gtctcctcga cctgccacag
     2701 atgatgcgca ttggcttcgt gctgaacatc gtaaccgcac tgagcctact cgcagtgctg
     2761 tacgcattga gcatgttgta cggctggact tagccttgcc gttttgttga gagacgcatc
     2821 gtgcgtgact catccttaca gttcacgact ttgtgagagg caaccatggt tactatgggc
     2881 actgtccctt ttcttcttaa tcaactgagg ccgcccggca aaggcggcgc ggcgctgacg
     2941 caggcagccg catcgatcgc gtttgcacaa ggtcagcccc aaacgatcgt ggacgtcttg
     3001 cacggccgcg cgtttcgcta ctacgcggaa ggtctgcgac aatatctcgc gattcgtacc
     3061 ggctctacgg agcgcgccga gcaatgcctc tccaagcttc gcgcctacgt ggcgaagacc
     3121 gaaaccgacg agctgattca agccccgggt gttcgtgcga gactctaccg cgccgcgcgc
     3181 ggatacatgc acaaccagag ccgcccgcac gagccgccca gcaggagcgc tctcgaagag
     3241 ctgccttggc gcgccctgcc ctcggcacac ctctcggtga gtcctttgaa cgcgcttcgc
     3301 ttcgacctca gcgcggaaga ctccgagctc ctcgagcttc ttcatgcgcg cgagctcagc
     3361 atcgaagagc tcgcgttcgt atacggcaag acggccaccg agatggaaga acgcgtcgac
     3421 gccgcaatgg cgcaggcgca gatcatcctc gacgagcatg gaatccgcga cgccgagcga
     3481 ttcgggcgag tcatcatcga agcgtttgcc ctcgaggccg ctccgcgcgc ccgaagcgac
     3541 gcagaggagg gtatcgaacc tctcccgaac ggaaccatcg tcggcggccg ctatgcaatc
     3601 gaagcccgcg tgggtctcgg cgcattcggt gacgtctacc gcgccaagga caccgaggtc
     3661 ccgggtcacg tcgtggcgct gaaattgctc caccaggcgg cgcattcgaa gtccgccaag
     3721 cagtcttcgc tgcgcgagtt gcggctgatt gcgtccgttt tccacccatc gatcgtgcag
     3781 ttcaaggacc atggctggta cgaagagcgg ctctggttcg tcatgccctg gtacgacggc
     3841 gagacgctcg agtcgcgcct tcaacgcgaa cctctgaccc gtgccgaggc gcttgcgatc
     3901 ttccagccgc tcgctcgcgc actggctgcc atgcacgccg cgggcgtgcg tcatcaggac
     3961 gtgaagccag agaacatttt tctcgcgcgc attcccggtt tcggtgaaga cgaagtcaca
     4021 cccgtgctgc tggatctcgg cgtcgcggcg acggaagcgg agatggtggt cgccggcacg
     4081 ccgacctatt tcgcgcccga ggtggctgcg cagttcgctt cggtggaaga caagcccagc
     4141 gtcaacaaca aggccgacgt gtttgcgctc gccctttcgc ttcgaaacgc gctcgatccg
     4201 gcgacgcaag aagacgtcgc cgcgggagct gtcgacacct tcatcgaata ccgttcgaga
     4261 gagacgccaa agccgcctac gctcaaagcg ctcaagtatc tcaccccgtt cttcgcgcgc
     4321 tggttgagcc gggaccccga ccagcgtccg agcgcagacg agtttgcgac cgagctctcc
     4381 gccttggccc ttcccgaaga gcgtcgtgaa cgcgtgaaga cgattctgcg ttggaccata
     4441 ccgatggcgc ttgcgctctt aggagcgtcc ggggctgcgg tgaccgtgtt ctatcagaaa
     4501 gccgaaacgc agcgtgagga ggcacatcag gccaagctga ccgcgttgga tctccgccgt
     4561 gatctggccg acacgaaaga gatcgcagag caactggagc ttcgctacga gcaaagccgt
     4621 ttgactcgaa cggacctggc gagacgactc gcgcgtacgg acggcgaggt cgtctccctc
     4681 aagacgcagc tcaaagacga agagcgccgc ttgatgcgca cgcgcgaaaa gctcacgcaa
     4741 accaggcagg agaaagccaa cgtagaccag agcctctccg aaaccgaacg cgagctcgcc
     4801 gccacggagc gaacgctctc tgcaaccaac cagaggctcg ccacgacctc tcagaagctc
     4861 tccgccgagg aggaccgtgt acggcggctg gaacgcaagc tcgagcgcga aggcgaacga
     4921 gtcatcgagc tctacgctca gatcgcggct ctggacgccc gtctgacagc cgaggcctcc
     4981 aagaccgacg agctacagga cgcagtcgcc cgtgcggtgg cggcgcgaag ccgggccgag
     5041 ctcgagctcg agttcgccca ggacgagatc aaggctttgc gtaatcggcc gacacctcat
     5101 gcaccggcgc ccgaagaaga gccacccacc aagcagagca gtcccgatag ctgaggctat
     5161 cggcggccct tgtagcccat catgtcttgc atcgcggact cgcccagctc ggcgatttct
     5221 gcttcctcag caaggcgctc agcgcagtcc tcgcacactt tgatgaggcg cccagccgct
     5281 ttgacggaaa cgagctcgtc tacctcgtcc ttgcacgatc ggcattccgg catgggtcta
     5341 tctcgcgcgc gggagaaaac ggctcatcga cggaagcacg acgttctggc gcacggagcg
     5401 tgccgcccca ccgagcccgc ctggaagtgc gcgaaatgcg atatctagat gcttcttgtc
     5461 ggtttcgact ccgtgcagag ttacgaacga agtgagcaag gacaccgcgc gtcggaccag
     5521 cgggttcttt ccgatcttcg gatcacgcat catgtcgagc acgattcggt tgccattggc
     5581 ctccgccaga acaggcgagc ggcccggcat gaaattcacc agggtgcccg gattcgaaag
     5641 gtccaaggcg ccagacttga tcagggccgc gatgggcgac tccgattcac cgttgagctt
     5701 gagcatcacg tcctcgagcc gtagagccac ggtcagctct tcgtcgttga agacgacgcg
     5761 ctcgacgtag atgaccgcgc ccgcgcgaat cggcgtattc atcaaatcga ggttgccgga
     5821 aaggcgcaag cctggaggga ccgaatcgat ttggaagtcc tcgaccttgc cgctgcgctc
     5881 tgcctgcgag cccagccagc gaagagcgac aagcggaacg ccgaaacgga ggcccaatgc
     5941 gctgcggaca gcgcgtccga gctcttcggg atgggtgcgc aaataggaac gaacggcgtc
     6001 gacggtttct tggggtgtgg gcatgaccgc aggagtctag tgttctgacg ggctcccgta
     6061 aacggtcaat tgttcacgag cgtcgtcggg tcgcagaaac cgcggttgtc gcctaagtcg
     6121 gtgcactcgg gctggatgtc caatacctgc gggcattggt tgatcacggc ggaattgcaa
     6181 agcctctggc aggtggggcc gttcttcccc tccccctcgc aagtcgctcc gggaatgcac
     6241 acacagacgg cacccacttc gcactcgatg ctgtaactgc aaatctggcc gatgatcctg
     6301 ggtacgaagt cagggtcctg gacgtctccg ggcatgcaga cccagagcgg ttcctccgcg
     6361 ccgccgccca caccaccctc accgccaacg ccgcccatgc cgccaacgcc gcccatgccg
     6421 cctgcgcctg cgtcaccacc ggaccctccg gccccatcgg aaagtggctc ggtctgcaga
     6481 caggattggc cgttctcgca gaaaaccgtt tcgacgctgc aagtctcgag gcagtagttc
     6541 ccgtagacgg tttcggcgca gaccgagccg ccgcgacatt cggacgactc gtagcacggg
     6601 tagccgtcat tggtcggacc cgaacaggcg gcgaagctcg ccgacgccaa ggcgagacaa
     6661 aacatgggga ctcgagcgct catcgagacc gtatgtagca agagagcccc aagcgggcga
     6721 tttttgtaca gtcagagcat cccggcccgt gggagcgacg aggcgttccc ttgccaaacg
     6781 acgtaccgct gggctttctg gtcataaacg aagagctccg ctccggcgtc gagcgggtcg
     6841 ctttcgagcg ccgacttgtc cggccgaaat ccgtccgtca tcggaatcga ttcgactcgt
     6901 ttgagccagg ccgggcgttc gctcgggcga agcccctcgg aaaacgcgtt ccatgcttcg
     6961 agatccaagc cccgatcgtg gacggtcgcc accactgcaa cggcgcggtc ggccccgtct
     7021 tcctcgaccc caaatacgac cgtctgacga agggcctcga acccgtagag ggcatcctcg
     7081 atcgaacggg tcgcgaccac gcctgctccg gtccgcaaca tgcgactcgc tcgatcgacg
     7141 aaccagtagt caccgtctgc atcgcggcgg agcacgtccc aggtgatgaa ccaagtgtcc
     7201 ttgggctcga acaccccttg gagtagccgc cggccggcct catcgccccc tgtgaaactc
     7261 gcgagtggat gctcggcatc gagtcgcgcg agcaaaatac cgggctcgtc atcttcacag
     7321 cgaaccagca gcccgtgctc accgcgaacg aactccttgt cctcgaagtc gtagcgcacg
     7381 agctccacct ccgcgcttcc gggaagcgga cgaccgaggg cgccgacttt ttcgccagac
     7441 gcgttggcga ggacggcgtt gccctccgtc gctgcataga actcgaggat gccgaccgga
     7501 ccaaaacgat cgacgacctg ccgccaaacg tcccggcgga gaccgcttcc cgcgaaaaga
     7561 cgaatcgggt tctcgttgtc ggcaaccgaa ggctcggtcc gcacgagctc tcgcaacatc
     7621 tcaccggcat aaaagaccac cgtcgcaccg tagcggcgga cgtcgtccaa gaacgttgtg
     7681 ggttcgaaac gggtcgcgag cgccaagcga gagccgccca ccaaaccgcc cccgaccgcg
     7741 acgagcatcc ccgcggggtg atgaagaggc aaacagcaat acactgtgtc gctaggcgaa
     7801 agcgtgcacg cagccgcggt cccatacgcg gaaaacgccc agcgctggtt ggtgatcttc
     7861 gccgcgcggg gcttcttccc gcgtcctgca gtcaggatga tgagcgccaa atcacgtgcg
     7921 caaccagggt ttggctcgta ccaagcgggg agctcgacgg cgcccgggtc gatcaactcc
     7981 atatcgacga cactgggcgg aagtttccgc tcctcgcgag cgccgccgag aacgagcact
     8041 tcgtcgaaga gctgttttcc gagcgccgcg ctttctgggt ccgcaatgag gaagcgcggg
     8101 gccgaggcat gaatggcctc ctcgagggtc tcgcgcggcc ggtctggact gagcaagatc
     8161 ggcacggcac cgagccggct cagggccgca gcaaccgaca gatagctcgg acgcggaccc
     8221 atcaagacgg caacgggctc acctcgtcga acgccgctcc gaatcagtcc gcgaacgacg
     8281 tgattgacgc gcctgtccgc ttgcgcgtag gaaaacgccc gcccttccca aagaaagaac
     8341 gtgccatcgg ggttcgacgt cgctttttcc gcaagggcgc gccccaggct cacgcgcgtg
     8401 tccggccgca tctgccggag cacgctcagc cgtggaagct gccagcgaag actgtcgagt
     8461 tgctcgccga agtcggtggc agaacgcccg atcctctgcc accagtttcc aagcgcgccg
     8521 acgatcgtcc ggtagaagag ctcgacgtcg aaatcgactt cgtcgaagtc cgcttcctct
     8581 ggctcttgca gtaccggcgg cgcgtcgagc accgagggtc gaggtccgag gccctctctc
     8641 cagcgaaccc aactcgccac ggtaggccaa gtgaacgaca tggcctgatt accg
//
LOCUS       DASRZF010000009         2126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_348354, whole genome shotgun sequence.
ACCESSION   DASRZF010000009 DASRZF010000000
VERSION     DASRZF010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348354"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VFG22_00380"
     CDS             complement(<1..223)
                     /locus_tag="VFG22_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414718.1"
                     /translation="MTLIADSDARLAPAKPVPRHKGHWLWGGTFDFQKNPAEFLLSAH
                     ERYGDVFVTRVLTGQSYFIRDPEVVNAINV"
     gene            complement(313..1272)
                     /locus_tag="VFG22_00385"
     CDS             complement(313..1272)
                     /locus_tag="VFG22_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414719.1"
                     /translation="MAVFDIFNGDADGICALTQLRNAEPLESTLITGVKRDIELVAKA
                     DLSEGDRATVLDISFDKNRAAVSKALDAGAEVFYVDHHFAGAIPEHEKLSTIIDPDPN
                     VCTSLLVNGYLKGRYVEWAVTGAFGDNLKASALRIAAPLDLTEQQLTLLESLGVFMNY
                     NGYGASLEDLHFAPAELFELVRHHPTPFAFVEEARDSFERLKSGYHHDMSLAASIKPT
                     RESESAAVYIFPNEPWARRVSGVYSNGLANDFPRRAHAVLTERPDGTYLVSVRAPLEN
                     KQGADDLCRQFPTGGGRAAAAGINALPQDQLDAFVDALARTYP"
     gene            complement(1281..2009)
                     /locus_tag="VFG22_00390"
     CDS             complement(1281..2009)
                     /locus_tag="VFG22_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET6414720.1"
                     /translation="MLDGRTIAVVVPAYNEAELIRDTLGSLPEFVDQIIVVDDASTDA
                     TGELVQVFFPSVVLLSHDDNRGVGAAIATGCRRALALGADFTAIMAGDGQMHPDDLPA
                     LLQPLIAEEVDFTKGNRLAWPSVRSEMPWHRWIGNQLFSSLTRQALGIDVHDSQCGYA
                     AMTRRASLAIEWDRLWRGYGYPNDLLSCLSLRGLRVRDVPVRPIYGAERSGIRLRHVA
                     CIVPFVIIRGWLRRTAPWLTSLVL"
BASE COUNT          430 a          640 c          651 g          405 t
ORIGIN      
        1 tgacgttgat cgcgttgacg acctccgggt cgcggatgaa gtaggactga ccggtgagca
       61 cgcgcgtcac gaagacgtcc ccgtatcgct catgtgcgga gagcaggaac tccgccggat
      121 tcttctggaa gtcgaaagtt ccgccccaga gccagtgccc cttgtggcgt gggaccggct
      181 tcgcgggcgc aaggcgggca tcagaatcgg cgatcaaagt catttactta acgtaaagta
      241 aacttatctg atgcgacccg caagttaaca agttgttttc ttaggccctc ggcgagcgtt
      301 gacactcgag cgctaaggat aagtgcgagc cagcgcatcg acgaaggcgt cgagctgatc
      361 ctgaggcagc gcgttgatgc cagccgctgc ggccctcccg ccaccggtag ggaactgcct
      421 gcagagatcg tcagcgccct gcttgttctc gagcggagcg cgaacgctca ccaagtacgt
      481 gccatcgggc cgttcggtga gcaccgcatg cgcccgtctc gggaaatcgt tggctagacc
      541 gttgctgtag acgcccgaaa cacgacgcgc ccagggctca ttcggaaaga tgtaaaccgc
      601 ggcgctttcc gattcccggg tcggtttgat cgatgcagcc aaggacatgt cgtgatggta
      661 cccagacttc aatcgctcga acgaatcgcg cgcttcctct acgaaggcaa aaggcgtcgg
      721 atgatgtcgc accagctcga agagctcggc tggagcgaag tgcaagtctt cgagcgaggc
      781 gccgtatccg ttgtagttca tgaagacgcc caggctctcg agcagcgtca gttgctgttc
      841 ggtgagatcc aggggcgcgg caatcctgag agcgctcgcc ttgaggttgt ctccgaaggc
      901 accggtcacc gcccactcga cgtaccggcc cttcaggtag ccgttgacga gaaggctcgt
      961 gcagacgttt ggatcggggt cgatgatcgt gctgagcttt tcatgctctg gaatggcgcc
     1021 ggcaaagtgg tggtcgacgt agaagacctc cgcgccggca tccaatgcct tcgacacagc
     1081 tgcccgattt ttgtcgaagc tgatgtcgag aacggtcgcg cgatcgccct cgctcaggtc
     1141 ggccttggcg accaattcga tgtctcgctt cacgccggtg atgagcgtgc tctccaaggg
     1201 ctctgcgttg cgaagctggg taagcgcaca gattccatcg gcgtcgccat tgaagatgtc
     1261 gaacacggcc atggacgcgt ctatagcacc aatgacgtta gccaaggcgc agtcctcctc
     1321 aaccagccgc gaatgatcac gaacggaacg atgcacgcca cgtgacgaag gcgaattccg
     1381 ctgcgctccg ctccgtagat cggccgtacc ggcacgtccc ggacgcgcaa gccacggagc
     1441 gacaagcagc tgagtagatc gttcggatac ccgtagcccc gccagagtcg atcccattcg
     1501 atcgcgagcg atgcgcgtcg ggtcatggcg gcgtagccgc actgggagtc gtggacatcg
     1561 atgccaagcg cctggcgcgt caaggaagag aagagctggt tcccaatcca gcgatgccag
     1621 ggcatttcgc ttctgacgga cggccaggca agccggttcc cttttgtaaa atctacctct
     1681 tcggcgatga ggggctgcaa caaggccggt agatcgtcgg ggtgcatctg cccgtcgccg
     1741 gccatgatgg cggtgaaatc cgcgccaagc gcgagcgcac gcctgcagcc cgtcgcgatc
     1801 gcggccccga ccccccgatt gtcatcgtgc gagaggagca cgaccgacgg aaagaacact
     1861 tgcaccagct cccccgtggc atcggtgctg gcgtcatcca cgacgatgat ttgatccacg
     1921 aattctggaa ggctccctag ggtatcgcga atcagctcgg cttcgttgta ggcgggcacg
     1981 acgaccgcga tggttcttcc gtccaacact ccggaacact caatcacttg ctccgagtgg
     2041 tcgcaaggcg tacctcaatt ggcaatggct caagttctct tcgtttcgaa gccggtcgtc
     2101 ccgccttgga acgacagcag caagaa
//
LOCUS       DASRZF010000010         8156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_348428, whole genome shotgun sequence.
ACCESSION   DASRZF010000010 DASRZF010000000
VERSION     DASRZF010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 8156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8156
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348428"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /gene="tgt"
                     /locus_tag="VFG22_00395"
     CDS             complement(<1..1136)
                     /gene="tgt"
                     /locus_tag="VFG22_00395"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012270584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HET6414721.1"
                     /translation="MTLPTPGFSFRVDAVDGNARSGELQTPRARIETPAFMPVGTQAT
                     VKGLTPEEVASTGARIILANTYHLWLRPGAESIAAHGGVVGFMNWPHALLTDSGGYQV
                     FSLAKHRTIDDDGITFRSHIDGSLQRLTPEEAMRVQALLGSDIAMVLDECPPGGAPRA
                     EVERAIERTTAWAERCLQTKPADGQARFGIVQGGTELDLRLSHLETVAALDFDGVALG
                     GFSVGEPSAQMYALLDRIAPQMPKDRPRYLMGVGTPSDLLQALSTGVDLFDCVLPTRN
                     ARNGQALTWGGRVNLKQARHREDESPLDEHCDCSVCTGYSRAYLRHLFKADEMLGPRL
                     LSLHNLHFYAALMRKAREMIRLGELGSWVASTLSEMKERDEVGDP"
     gene            complement(1133..2176)
                     /gene="queA"
                     /locus_tag="VFG22_00400"
     CDS             complement(1133..2176)
                     /gene="queA"
                     /locus_tag="VFG22_00400"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HET6414722.1"
                     /translation="MRITDLDYVLPDELIAQEPLAKRDASRLLLLDVPTGSIEDHRFT
                     ELPQLLAPSLFIFNDTRVFPARLIGNKASGGRVELLLLRKATHGQDRWLAMGRSSKGL
                     REGMDLTLCEGRLNARVLRVLEHGQLEVDLSSDAEVDRVIEEAGEVPLPPYIRRAASE
                     SDRGRYQTVYARNPGAVAAPTAGLHFTETTLSALEGAGHWSAYVTLHVGPGTFRPVQA
                     DTLDEHEMHEETYEVPASTAATIKRARDEGRPVVAVGTTVVRTLESCVGEDGMPSAGV
                     GATRLFIRPPYRFRIVDHLVTNFHLPRSTLLALVMAFAGTELTRSGYRHAAEQRYRFY
                     SYGDAMLVRRAPR"
     gene            complement(2179..3990)
                     /locus_tag="VFG22_00405"
     CDS             complement(2179..3990)
                     /locus_tag="VFG22_00405"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6414723.1"
                     /translation="MSSLPRPGDIIAERYRVIAAIGRGAMGTVYRAEHVTISKVMAIK
                     LLHQELQQDPENVARFHREAESASRLNHPNTVHVFDFGRTKSGALYLVMEYVEGDDLA
                     KIIDKDGPMAFGRVAFLLAQVAGSVADAHAAGIVHRDLKPENIVVTEGRDGETAKVLD
                     FGLAKLFEGTVEAQVTSSGTIVGTPFYMSPEQIQGQDLDGRSDVYAMGAIMYECVVGR
                     PPFEAPNPVGVLSKHLSEEPIRPSARSPLSVPAEADEIIMRCLAKDRNERYQTAEDLR
                     QELIDYLGTVGSGDWRSSAVGKPPFGNGARRVDAIDPALKPKRRLWWRLLLLVLLGGA
                     VAAWQLATRGPSEREPNHSEKNANPLLENSEMKAYLGQRLSEKSGDVDSFEIEYSGAE
                     RRAVELDVSSIPNMDVLIELLKPEQERPMIVGDSKGLGQGERLPNVPIEPGRYLIRVR
                     ERAIDGRMPTENVSDPYFIRWRLLDPDLGYEREPNDSLELAESIGLGTERRGWIGWSG
                     DVDTYCLSENAERVVAQVSALAEVDLVLRVVDQRTDRSTKYDDKSVGRGETSKPWRSA
                     EPQTLCVEVSADPREEDGRPAQPDTTYGVRFITAPGP"
     gene            complement(4082..4690)
                     /locus_tag="VFG22_00410"
     CDS             complement(4082..4690)
                     /locus_tag="VFG22_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET6414724.1"
                     /translation="MDCPQCGAVVPSDDLFCGKCGYAMRDEGPERLDRSRIRMHEEPE
                     WPAGDSQSRSSSQQRVRKHTVLGMPSVTPPRGSRPPTPAPAPVVPSPPTSITSARAGG
                     SRTRTPQKTMLGIPRHEIPEPPSDPSSAESAVDEPAHASEESAPSEATTPSHRVRARV
                     RYDSANEPFPVTQRRKKALLALAILVLFASAWLGYRYLTLNG"
     gene            complement(4753..5559)
                     /gene="dapB"
                     /locus_tag="VFG22_00415"
     CDS             complement(4753..5559)
                     /gene="dapB"
                     /locus_tag="VFG22_00415"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HET6414725.1"
                     /translation="MVQVAIHGAAGRMGQSIAAVLNEDRDTNLVAAMDHEGCGLVGKD
                     MGALLGAARSGVTITSDVEEFLSNVEVVIDFSIPSATAKLLPLCAGQHIPMVIGTTGL
                     DAATRAALERAAERVPVVFAPNYSQGVNALYFLAARATELLGPGFDAEIVEIHHRHKV
                     DAPSGTAVRLGEVVARAKGLDPERAVSHGRSGQVGARPLEEVGVMALRGGDAVGEHTL
                     YLVGEGERIELTHRATDRSIFARGAVRAAHWIVTRPAGLYDMADVMGIPR"
     gene            complement(5603..6763)
                     /gene="tyrA"
                     /locus_tag="VFG22_00420"
     CDS             complement(5603..6763)
                     /gene="tyrA"
                     /locus_tag="VFG22_00420"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF008400.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HET6414726.1"
                     /translation="MTDENPDKGPLLELRGALDALDHELLELLVRRMDIVAEIAVRKR
                     SHRVRIRDLARERRVLEDRCARAQELGLPTGGIESVWRQLMLMSRERQAALRAEVPLD
                     VESKSIAIIGGEGGMGRSLNTLFSDLGHEVLSADLETPLRPEQVAATADVVIISVPIR
                     DTRSVIEQLGPLVREDALLMDVTSIKTDPMEAMLASTDASVLGTHPMFGPGVNTYQGQ
                     RVVICPGRGDVWLDWARQMFTARGLVITETTPSEHDSMMAIVQVLHHFKTQVLGLALS
                     RAGAPLEETLRFTSPAYLLEAYVTGRHFAQSPDLYGPIEMLNPDSERIIKGFQEAAAH
                     LAGVLATGDQKAFVQVFAEVREFFGDFTDEALEQSGFLIDRLVELSAGRSSS"
     gene            6809..8071
                     /locus_tag="VFG22_00425"
     CDS             6809..8071
                     /locus_tag="VFG22_00425"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HET6414727.1"
                     /translation="MKRLQLHHWILIAMSLGAVLGLALNWAGGQGMVDASIVGNVALV
                     GKAIGDLFLRLLQMLVVPLIISSLITGVTGMGDLRTLGSMGGRAIAFYISTSFLAIVT
                     GIVMVNLIRPGDGAELALLEEGAATGALLAEAPKGIGLVLWEQLEGLIPKNPLAAATE
                     GDMLPVIFFALLIGIFISVAEHREGSKDASIVRRFFSGLFDVMMQMTLAVVKFAPIGV
                     FGFTLYAAAGKGPAAFQVLGWYVLTVFIALVIHATITLPLILKLRTGRSPLAYARTLT
                     TALVTAFSTASSNGTLPLTMENVERAGVRPQVTAFVLPLGATINMDGTALYEAVAVLF
                     IAQVYGIDLSLTQQGLVAFTALLASIGAAGIPHAGMVMMVVVLNAVGLPTSAVALILA
                     VDRILDMCRTTVNVWSDSIAAAVVDAGE"
BASE COUNT         1442 a         2656 c         2541 g         1517 t
ORIGIN      
        1 ggatcgccga cttcgtcccg ctctttcatc tcgctcaggg tcgacgcgac ccaggaacca
       61 agctcgccga gtcgaatcat ttcgcgcgct ttgcgcatga gcgcagcata gaagtgcagg
      121 ttgtgaagcg agagcagtct cggcccgagc atttcatcag ccttgaaaag atgccgcaaa
      181 taggcacgtg aatagcccgt gcagaccgag caatcgcagt gctcgtcgag cgggctctcg
      241 tcctcgcggt gcctggcctg cttgagattg acgcggccac cccaggtcag agcctgaccg
      301 ttacgcgcgt tgcgcgtcgg aaggacgcag tcgaaaagat cgacaccagt cgaaagcgcc
      361 tgcagcagat cgctcggagt gcccacgccc atgaggtacc ggggtcggtc cttcggcatc
      421 tgcggcgcga tgcgatcgag gagggcgtac atctgcgccg aaggctcgcc gactgagaag
      481 ccaccgagcg cgacaccgtc gaagtcgaga gcggcgaccg tctccaaatg agagagccgt
      541 agatcgagct ccgttcctcc ttgcacgatg ccgaatcgag cctggccgtc cgccggcttc
      601 gtttggaggc agcgttcggc ccaggccgtg gttcgctcga tcgcgcgctc gacctccgct
      661 cgcggcgccc ctcctggagg acattcgtcg agcaccatgg cgatgtcaga gcccaggagc
      721 gcctggacgc gcatggcttc ttcgggcgtc agacgctgga ggcttccatc gatgtgagag
      781 cgaaacgtga ttccatcgtc atcgatggtg cgatgcttgg cgagcgaaaa cacctgataa
      841 ccgccgctgt ccgtcagcag cgcgtgcggc cagttcatga aaccgacgac tccgccatga
      901 gctgcaatgg actcggcgcc gggtcgcagc cagaggtgat aggtgttggc caagatgatg
      961 cgcgcaccgg tggacgcgac ctcctcgggg gtgagcccct tgaccgtggc ctgggttccg
     1021 acaggcatga aggccggagt ttcgattcgt gcccgaggcg tctgcagctc tccagagcgc
     1081 gcgttcccat ccacggcatc gacgcgaaac gagaagccag gcgtggggag cgtcatcgcg
     1141 gcgcccttcg aaccaacatg gcatctccgt agctgtagaa gcgatagcgc tgctcggcag
     1201 cgtgacgata gccgctgcga gtgagctcgg ttccggcaaa cgccatcacc agcgcgagga
     1261 gagtcgagcg tggaagatga aagttggtga ccaagtggtc gacgatgcga aagcgatatg
     1321 gcggacgaat gaagagccga gtcgcgccca cacctgcgga cggcatgccg tcctcgccaa
     1381 cgcacgattc gagcgtccgg acgaccgtag tgcccactgc aacgacgggt cgcccctcgt
     1441 ccctggcacg cttgatggtg gccgccgtgc ttgcggggac ttcgtatgtc tcttcatgca
     1501 tctcgtgctc atccagcgta tcggcttgaa ctggacggaa tgtgccgggt ccaacgtgca
     1561 gtgtcacata ggccgaccaa tgcccagctc cctcgagcgc gctcagcgtg gtctcggtga
     1621 agtgcagccc tgccgtgggc gccgccacag cgcccggatt tcgagcgtag accgtctggt
     1681 agcgtccccg atccgattcg cttgcggcac gccgaatgta gggaggcaag ggaacttcgc
     1741 cggcctcttc gatgactcgg tccacctcgg catcactgct gaggtcgacc tctagctgtc
     1801 catgttcgag tacgcgaagc actcgagcat tcaatcggcc ctcgcagagc gtgagatcca
     1861 tcccttcgcg caagcccttc gaggagcggc ccatcgcaag ccagcggtct tggccgtggg
     1921 tggctttgcg cagcaggagc agctcgacgc gacctccgct tgctttgttg ccgatgagcc
     1981 gcgcgggaaa gacgcgtgtg tcgttgaaga tgaagaggct cggagcgagc aactgcggta
     2041 gctcggtgaa tcgatggtct tcgatcgaac ccgtgggcac gtcgagcagg agcagacgcg
     2101 atgcgtctct cttcgccagc ggctcctgcg cgatgagctc gtcgggcaag acgtagtcga
     2161 ggtcggtgat tcgcattcct aaggcccagg ggctgtgatg aagcgcactc cgtaggtcgt
     2221 gtcgggctgc gcggggcgac cgtcttcctc gcgcggatcg gccgagacct cgacacagag
     2281 cgtctgcggt tccgcgctgc gccagggttt gctcgtctca ccacgtccca cgctcttatc
     2341 gtcgtacttc gtgctgcgat cggtgcgctg atcgacgact cgaagaacga gatcgacctc
     2401 ggcgagcgca ctcacctgcg cgaccacccg ctcggcgttc tcactcaggc aataggtgtc
     2461 gacgtcgccc gaccatccaa tccagccgcg gcgctccgta ccgagtccaa tagactccgc
     2521 gagctcgagc gaatcgtttg gctcgcgttc gtagcccagg tccggatcga gaaggcgcca
     2581 acggatgaaa tacgggtccg agacgttctc ggtaggcata cgcccgtcga tggcgcgctc
     2641 tcgcacgcga atcaagtacc gaccgggctc gatgggtacg ttgggcagtc gctccccctg
     2701 ccccagccct ttggagtcgc cgacgatcat gggccgctcc tgctccggct tcaacagctc
     2761 gatcagtacg tccatgttgg gtatcgagct gacgtcgagc tccacggcac ggcgctcggc
     2821 cccggagtac tcgatctcga atgaatcgac gtcacccgac ttctcgctga ggcgctgacc
     2881 gagataggcc ttcatctcgc tgttctcgag cagcggattc gcgttcttct cgctgtgatt
     2941 gggctcccgt tccgacggcc cgcgagtcgc aagctgccag gcggcgacgg cgccgccgag
     3001 gagaaccagc agcaagagcc tccaccaaag ccttcgcttc ggtttgaggg ccgggtcgat
     3061 cgcatcgacg cgccgagcac cgttgccgaa tggaggtttt ccgactgcac tcgagcgcca
     3121 atctcccgag ccgaccgtgc cgaggtagtc gatcagttcc tgcctgagat cctccgccgt
     3181 ctggtagcgc tcgttccggt ccttggcgag acagcgcatg atgatctcgt cggcttccgc
     3241 cgggacggag agcggtgagc gcgccgaggg acgaatcggc tcctcggaga gatgcttcga
     3301 aagaactcca acgggattgg gcgcctcgaa ggggggtctt ccgacgacgc attcatacat
     3361 gatcgcgccc atcgcataga cgtcacttcg cccatcgagg tcctgaccct gaatctgttc
     3421 gggggacatg tagaatggcg tgcccacgat ggtcccgctg ctggtcacct gagcttcgac
     3481 cgttccttcg aacagcttgg cgagcccgaa atccagtacc ttcgccgtct ccccatcgcg
     3541 gccttccgtg acgacgatgt tttcgggctt tagatcgcga tgaacgatgc ctgcggcgtg
     3601 ggcgtcggcc accgatcccg cgacctgagc caagagaaat gcgacgcgcc caaacgccat
     3661 gggcccgtcc ttgtcgatga ttttggccag atcatcgcct tccacgtact ccatcacgag
     3721 gtagagcgcg ccggacttgg tgcgcccgaa gtcgaacaca tgaaccgtgt tcggatgatt
     3781 gaggcgcgac gccgactcgg cttctcggtg aaagcgcgcg acgttctctg ggtcttgctg
     3841 aagctcctgg tgaagcagct tgatcgccat caccttgctg atcgtaacgt gctcggcccg
     3901 atacacggtc cccattgcgc cgcgcccgat ggctgcgatc acccgatagc gctccgcgat
     3961 gatgtcgccg ggcctgggca acgaagacac gatcggtgtt ccgcacttgc cacagaactt
     4021 cgactccgga gggttgccct ccccgcatgc atgacaatgc gactggctgc ctggcgctgt
     4081 gctacccatt caatgtcaga tagcggtagc ccagccacgc tgaggcgaac agaacgagga
     4141 tcgcgagagc tagaagggcc ttctttcttc gctgggtcac cgggaacggc tcgttcgcgc
     4201 tgtcgtaacg gacgcgcgcg cgcacgcgat gacttggcgt ggttgcttca ctcggcgcgc
     4261 tctcttcgct ggcatgggcg ggttcatcga cggctgactc cgccgatgat ggatcgctcg
     4321 gtggctccgg gatctcgtgg cgcgggatgc ctagcatcgt cttttgcggc gttcgcgttc
     4381 gcgaacctcc ggcacgtgcc gatgtgatcg aagtcggcgg cgacggcacc acgggtgccg
     4441 gtgctggtgt cggcggacga cttccacgcg gcggtgtgac cgagggcatt ccaagcacgg
     4501 tgtgcttgcg aacgcgttgc tgcgaagagg agcggctctg cgaatcccca gccggccatt
     4561 cgggctcctc gtgcatgcga attcgcgacc ggtccagccg ttccggcccc tcatcgcgca
     4621 tggcgtaccc acacttgccg cagaacaagt catcggacgg aactacggct ccacattgag
     4681 gacaatccat aacgcgcccc taccgtatca gaattcgtaa gagctggctt cgtacttgct
     4741 tcgcgcttgg cactagcggg ggatccccat gacgtccgcc atgtcgtaga gcccggcagg
     4801 ccgagtcacg atccagtggg cggcacgcac ggcgcccctc gcaaagatcg agcggtctgt
     4861 tgcgcggtgg gtgagctcga tgcgctcacc ttcgccgacg aggtagaggg tgtgctcgcc
     4921 caccgcgtcg cctccgcgaa gggccatgac gccgacctct tcgaggggcc ttgctcccac
     4981 ctggccgctt cgcccgtgac tcaccgcccg ttcaggatcg agccccttgg cccgcgcgac
     5041 gacttcgccg agacgaactg cagtgccgct cggagcatcg accttgtgcc gatgatgaat
     5101 ttccacgatc tccgcatcga agcccggacc gaggagctcg gtcgcgcgcg ccgcgaggaa
     5161 gtaaagagca ttgacgccct ggctgtagtt gggggcgaac acgaccggca cgcgctcggc
     5221 agcccgctcg agcgccgcac gagtcgcagc atcgagcccg gtcgtcccga tcaccattgg
     5281 tatgtgctgt ccggcacaga gcggaagcag ctttgcggtc gcggacggga tgctgaaatc
     5341 gatcacgacc tcgacgttgc tgaggaactc ctcgacatcc gaagtgatcg tgacgccgct
     5401 ccgcgccgcg cccagcaggg cgcccatgtc cttaccaacg agcccgcagc cttcgtggtc
     5461 catcgccgca acgaggttcg tatcgcggtc ttcgttcaga acggcagcga tgctctgtcc
     5521 catccggccc gcggccccat gtatcgcaac ctgcaccatg ccgatggcat cgcagatgcg
     5581 gcgacgagcg tcaatcgagc cgctagctcg aggagcggcc cgcgctcagc tcgacgagac
     5641 ggtcgatcag aaaaccgctc tgctcgagcg cttcatccgt gaagtccccg aagaattcgc
     5701 gcacctcggc aaagacctgg acgaatgcct tctggtcacc ggtcgcaagg accccggcga
     5761 gatgcgcggc agcctcttga aatcccttga tgattcgttc ggagtcggga ttgagcatct
     5821 cgatcggccc gtagaggtct ggggactgcg cgaagtgacg gccggtgacg taggcctcga
     5881 gcaggtaagc cggcgaggtg aatcggagcg tttcctcgag cggtgcgcca gcacggctca
     5941 gcgccagccc gagcacctgg gtcttgaaat gatggagcac ctgcacgatg gccatcatcg
     6001 aatcgtgctc cgaaggggtc gtttcggtga tgaccaggcc gcgcgccgtg aacatctgcc
     6061 gcgcccagtc gagccacaca tcgccgcgac ccggacagat caccacgcgc tgaccttggt
     6121 acgtattgac ccccgggccg aacatcggat gggtgccgag cacgctcgcg tccgtggagg
     6181 caagcatggc ttccatggga tcggtcttga tcgaggtcac gtccatcaga agtgcgtcct
     6241 cgcggaccaa gggtccgagc tgctcgatga ccgatcgtgt gtcgcgaatc ggcacactga
     6301 tgatgactac gtccgcagtc gccgcgactt gctctggccg aagcggtgtc tcgaggtcag
     6361 cgctcaacac ctcatgaccg aggtcggaaa agagagtgtt gagcgagcgt cccatgccgc
     6421 cttcaccgcc gatgatcgcg atgctcttcg actcgacgtc gagcggcacc tcggcgcgaa
     6481 gcgcggcctg ccgctcccgg ctcatcaaca tcaactgacg ccagaccgat tcgatgcccc
     6541 cggtcggaag accgagctcc tgggcgcggg cgcagcgatc ctcaagaaca cggcgttccc
     6601 gggcgaggtc tcgaatccga acgcgatgcg atcgcttccg aaccgcaatc tccgccacga
     6661 tgtccatccg gcggaccagc agctccaaga gctcatggtc gagggcgtcg agcgcgcctc
     6721 tgagctccaa aaggggccct ttgtctggat tctcgtcggt catgggttgg ttttacatcg
     6781 aacctgggcg tccgcataat gcgcgcccgt gaaacgactg caacttcatc attggatcct
     6841 gatcgccatg agtctaggcg ccgtcctcgg gctcgcgctc aactgggcag gcggccaggg
     6901 catggtcgac gcgagcatcg tcggaaatgt agcgctggtc gggaaggcga tcggggacct
     6961 cttccttcgg cttctgcaga tgctggtcgt ccccttgatc atctcctcgc tgatcaccgg
     7021 cgtgaccggc atgggcgacc ttcggactct cggctccatg ggcggaagag cgatcgcttt
     7081 ttatatctcg accagcttcc tcgccatcgt gaccggcatc gtgatggtga acctcatccg
     7141 tcccggcgac ggggccgagc tcgcccttct cgaagagggg gcggcgacgg gagcgcttct
     7201 ggcagaggcg ccgaagggga tcggtctggt cctatgggaa caactcgagg gcttgatccc
     7261 aaagaacccc ctcgcagcgg cgaccgaagg tgacatgctg ccggtgatct tcttcgcact
     7321 tctgatcggc atcttcatca gcgtcgccga gcaccgcgaa ggctcgaagg acgcaagcat
     7381 cgtgcgacgt ttcttctcgg gtctcttcga cgtgatgatg cagatgaccc tcgctgtcgt
     7441 gaagttcgcg ccgatcggtg tctttggctt cacgctctat gccgccgccg gcaagggccc
     7501 tgccgcgttt caggtccttg gctggtatgt gctcaccgtc ttcatcgctc tcgtgattca
     7561 cgccaccatc acgctgcccc tgatcctcaa gcttcgcacc ggccgctcgc cactcgcgta
     7621 cgcgcgcaca ctcaccaccg cgctcgtaac cgccttcagc accgcttcga gcaacggcac
     7681 cctccccctc accatggaaa acgtcgagcg ggcgggcgtc aggcctcagg tcactgcctt
     7741 cgtgcttcct ctcggcgcga cgatcaacat ggacggcacc gcactttacg aagccgtcgc
     7801 ggtcttattc atcgcccagg tttacggcat cgacctaagc ctcacccagc aaggcctcgt
     7861 cgccttcacg gccttgctcg ccagcattgg tgcggcagga atcccgcacg ccggcatggt
     7921 catgatggtc gtggtcctca acgcggtggg cctaccgacc agcgcggtag cgctgattct
     7981 cgccgtcgac cgcatcctcg acatgtgccg aaccaccgtc aacgtttggt ccgactcgat
     8041 cgcggccgcc gtcgtcgacg ccggcgagta gcgcagacgc tctcaacgtc cggcgagatc
     8101 ggaaacaccg gcggccaacc gcttcgggaa ccaggggccc tcgtagacta gcgccg
//
LOCUS       DASRZF010000011         6700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152 k141_4164,
            whole genome shotgun sequence.
ACCESSION   DASRZF010000011 DASRZF010000000
VERSION     DASRZF010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 6700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6700
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4164"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1810)
                     /locus_tag="VFG22_00430"
     CDS             complement(<1..1810)
                     /locus_tag="VFG22_00430"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HET6414728.1"
                     /translation="MTLQGTMLSRIEGWAKSSGSEAAIHDRNPDGTWATSTWADYWAS
                     ARRVGKGLMALGLEPKDCVAIVGANRRDWVICQHGINAAQGIPAPLYTTLLPEQMAYI
                     IENAQSKIVICDDQTQLDKYLSIAKGDTPIEHIVTMDDLGSTDPRVITLDALMQKGVS
                     VSDAELDARLDAVKMDDVALLIYTSGTTGLPKGAMLTHAGIDLIAHAVAEVFPSLLNT
                     KTRSVSYLPLCHAAEQGMTNFAGLITKAETYYCGDLTQIKDYLIEVHPTIFLAVPRVW
                     EKFESALRGKLAEATGIKAKLASWARATELAAFKEEAATGRPVTSFSRNLANKLVISK
                     IKDALGLDQLQVALSGAAPISVSTLEFFASIGLPIHEGYGMTETTAFASVQPYGHLRF
                     GTIGKPLPGVQAKIADDGEIMLRGINMVKGYLRLPDKTAELYDEEGWMHTGDLGAIDE
                     DGYISITGRKKDLIITAGGKNVAPAEMEGYLQSIPGVGQAVVVGDRQPYLSALIILDA
                     EALPELEVAAQISGLTDVATAARNPDVKRFIEEEMQQVCNAKVARYQTIKKIKILPTT
                     FSVDGGELTPTLKVKRNIVNEKYAKEIAAFYAESAPA"
     gene            complement(1934..2023)
                     /locus_tag="VFG22_00435"
     tRNA            complement(1934..2023)
                     /locus_tag="VFG22_00435"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1987..1989),aa:Ser,seq:tga)
     gene            2139..3929
                     /locus_tag="VFG22_00440"
     CDS             2139..3929
                     /locus_tag="VFG22_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HET6414729.1"
                     /translation="MATDTIPHRLLQRAKKTPNAPAYYVRDKGSWKPTNWGTYSDQVT
                     QAGRALIALGFEPGQTVCILGFNRPEWAILDIAAMGAGGAPAGIYTTCSPVEVRYIVD
                     HAEAPLILVENEEQWHKVRAERANMPKLKYVVTMKGAPAIADDMVMSWDEFLAKGNEV
                     DKEAYVTRVESLEPTGLATLIYTSGTTGPPKGVMLSNHNLAWTSNAAIQITHIDATDC
                     SLSYLPLSHIAEQMFTLHIPITTGARVYFAESIEAVPENLKEVQPTIFFGVPRIWEKF
                     HAGIASKLKAATGAKAKLVQWAMKVGWEANKTPASHKGLQYRLADKLIFSKLKPAIGM
                     GNARVCVTGAAPIAREILEFFASLDLVILEVYGQSEDSGPTSFNTPTQYRFGSVGPAI
                     PGVEIKIADDDEILVKGPNVFMGYYKDPETTDETLSNGWLHSGDLGAFDDDGFLNITG
                     RKKDIIITAGGKNITPKNLEAAIKNHPLVDEAVVIGDRRKYLSALVTIDAEAGPAWAK
                     ENGEDPTALDKSAKLRASIQAHIDEMNAHFARVEQIKKFKILHRNLTVEDGELTPTLK
                     VKRAKVNDHFAEDIESMYVEDAGTQPQARA"
     gene            complement(3926..4342)
                     /locus_tag="VFG22_00445"
     CDS             complement(3926..4342)
                     /locus_tag="VFG22_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HET6414730.1"
                     /translation="MSARSKTIAIETRGRGTTEITEQIHAAVAESGIREGLCTVFIHH
                     TSASLIVNENADRDVQRDLEAFLTRLVPDGDSLYVHTMEGPDDMPAHVKTALTQTSLS
                     IPVMDGRCALGTWQGVFLWEHRRRGHQRRVTVTVVG"
     gene            complement(4339..4812)
                     /locus_tag="VFG22_00450"
     CDS             complement(4339..4812)
                     /locus_tag="VFG22_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414731.1"
                     /translation="MPLDPEFVADCPYGPGAICIDELVEVDSEAGIVRARMSTHPDLP
                     LTRDQRAHPIRHPRHVSGGFMVHLTGILGFMHTYYVLGLRHADGWIGYGGRIYDARFA
                     ALAKMGPPLDLELRATKIRQRENQVFGRYEFRFTQDDILIYQGDQAAMWMRVSAP"
     gene            complement(4816..5472)
                     /locus_tag="VFG22_00455"
     CDS             complement(4816..5472)
                     /locus_tag="VFG22_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET6414732.1"
                     /translation="MAALDQIRIHEIYESIQGESTFAGLPCTFVRLSRCNLRCRWCDT
                     PQAFEGGTEMPRAEVLRKALSFGTPLVELTGGEPLLQPAALPLLAELCDSGRTVLLET
                     SGERDISEVDPRVHRIMDLKAPGSDESHRNRWENIEQLTQRDEVKVVLADRSDYDWAK
                     GVIAEHDLAERVHAVLLSCVWGELDPKELVRWVLADRLPVRVQVQMHKVIWDANAQGV
                     "
     gene            complement(5475..>6700)
                     /locus_tag="VFG22_00460"
     CDS             complement(5475..>6700)
                     /locus_tag="VFG22_00460"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET6414733.1"
                     /translation="AGPAQAAPFELDAPVAEHVLEIRAGNPDLRDDVFSKMGGSSVES
                     KAFIHCFASPYVDLGEHEALSGTLDYFNAGELNSFERESHAAGVSWNLRYVLGGRPAK
                     FHRELEETQARWALVLFGGNDAQNENERIYVGRLVYLIEQLEGMGVVPVLGSALPRRN
                     PNRDRWISRFNAITEAVAEHWSLPYIDYHAALDALPRKGLARDGVHPNVLGEGGVRAA
                     CQLTKKGLRYGNNVRNLLTLEMLDALRRVVAGTGAGTDADAGTDAGTGTGTDAGTGTG
                     ADTDAGTDAGTDTVAVPDPFPVSALVSKSSLPLAGSLPERCGAPGPGSRVYRFRVELE
                     ERSRLRASAFDLDGHRFPVYWVRVDPDGERCVRRRLETLEVDAQPGMWDFIVEVPERA
                     AQEGQMLVLITRNPR"
BASE COUNT         1310 a         2124 c         2041 g         1225 t
ORIGIN      
        1 ccgccggggc gctctctgcg tagaacgctg caatctcctt cgcgtacttc tcattgacga
       61 tgttgcgctt cactttgagg gtgggcgtga gctcacctcc atcgacgctg aacgtggtcg
      121 ggagaatctt gatcttcttg atcgtctggt agcgcgccac tttggcgttg caaacctgtt
      181 gcatctcctc ttcgatgaag cgcttcacat cggggtttcg ggctgcggtg gccacgtcgg
      241 tgaggcccga aatctgggct gcgacttcga gctctggcag cgcctccgcg tcgagaatga
      301 tcagagcgga gagatacggc tgcctgtcgc cgaccaccac tgcctgaccg acgccgggaa
      361 tcgactgcaa gtaaccttcc atctcggccg gcgcgacgtt cttgccaccc gctgtgatga
      421 tcaggtcttt ctttcgaccg gtgatcgaga tgtagccgtc ctcgtcgatg gcgccgaggt
      481 cgcctgtgtg catccagccc tcctcgtcgt agagctctgc ggtcttgtcg ggcaagcgga
      541 ggtaaccctt caccatgttg atgccacgga gcatgatctc gccgtcgtcg gcgatcttcg
      601 cttggactcc cggaaggggc tttccaatgg tgccgaatcg aagatgccca tagggttgca
      661 ccgaggcgaa cgccgtggtc tcggtcatac cgtacccctc gtgaatcggg agcccgatcg
      721 acgcaaagaa ctcgagggtc gagaccgaga tcggcgccgc gccactcagg gccacttgga
      781 gctgatccag accgagcgcg tctttgatct tcgaaatgac gagcttgttg gcgagattgc
      841 gcgagaagct cgtcaccgga cgccccgtcg cggcttcttc cttgaatgcc gccaactcgg
      901 tggcgcgagc ccagctcgcg agcttcgctt tgatcccggt ggcctcggcc agctttccgc
      961 gaagcgccga ctcgaacttc tcccagacgc gcggcaccgc gaggaagatc gtcgggtgca
     1021 cctcgatcag gtagtccttg atctgcgtga ggtcgccgca gtagtacgtc tcggccttgg
     1081 tgatcaggcc tgcgaagttg gtcatgccct gctcggctgc gtggcagagc ggaaggtagc
     1141 tcacggaacg cgttttcgtg ttcaggagac tcggaaacac ctccgccacg gcgtgggcga
     1201 tgaggtctat cccagcgtgg gtgagcatcg cccctttcgg aagaccggtc gttccggagg
     1261 tgtagatcag gagcgcaacg tcatccatct tcaccgcgtc gagccgggca tcgagctctg
     1321 cgtcgctgac cgaaacgccc ttctgcatca gagcgtcgag ggtgatgacg cgcggatcgg
     1381 tggatccgag atcgtccatc gtgacgatgt gctcgatggg agtgtcgccc ttcgctatgg
     1441 acaagtactt gtcgagctgc gtctggtcgt cgcagatgac gatcttcgat tgcgcatttt
     1501 cgatgatgta ggccatctgc tcgggcagaa gggtcgtgta gagcggcgcc ggaattccct
     1561 gcgccgcatt gatgccatgc tggcagatca cccagtcgcg ccgattggcc ccaacgatgg
     1621 ccacacagtc cttgggctcg agcccgagcg ccatgagccc cttgccaacg cggcgtgcgc
     1681 tcgcccagta atcggcccaa gtcgaagtcg cccaggttcc atccgggttc cggtcatgaa
     1741 tcgcggcttc gctgccactg gacttcgccc aaccttcgat ccgcgagagc atcgtgcctt
     1801 gcaacgtcat agagcctccc aaagagagcc cacgctaccc gatccgatgg gatgatcaat
     1861 agacgccggc actgacgctg gcactgacgc tgacgctggc actgacgctg gcactggcac
     1921 tgacgctgaa aactggcgga ggaggaggga ttcgaacccc cggtagagtc gcccctacaa
     1981 cggatttcaa atccgccgcc ttcgaccgct cggccactcc tccgtggatt gagcgattta
     2041 gcacaaggtt ttgggggtgc ccttcacatt tgaggtgatt tcggcctata ttgcccggtc
     2101 ccggccggcg gacggctgga aactcaagag gtgacgctgt ggctactgac acgattcccc
     2161 atcgattgct gcaaagagcg aagaagacgc cgaacgcacc ggcgtactac gtccgagaca
     2221 aagggagctg gaagcccacg aactggggca cctactcgga ccaagtgacg caggcagggc
     2281 gcgccttgat cgcgctgggt ttcgaaccgg gacagacggt ctgcatcctc ggtttcaatc
     2341 gtccggagtg ggccatcctc gacattgccg caatgggcgc agggggcgca ccggccggca
     2401 tctacacgac ctgctctcct gtcgaggttc gctacatcgt cgatcacgcc gaagccccgc
     2461 tcatcctcgt cgagaacgag gaacagtggc acaaggttcg agccgagcgc gccaacatgc
     2521 ccaagctcaa gtacgtcgtc acgatgaaag gcgcccccgc catcgcagac gacatggtga
     2581 tgagctggga cgagttcctg gccaagggca acgaggtcga taaagaagcg tacgtcacgc
     2641 gcgtcgaaag cctcgaaccg accggtctgg caacgctgat ctacacctcg ggcacgaccg
     2701 gcccgcccaa gggcgtaatg ctaagcaatc ataacctcgc ctggacctcg aacgccgcca
     2761 tccagatcac tcacatcgac gcgacggatt gctcactctc gtatcttccg ctctcacaca
     2821 tcgccgagca gatgttcacg ctgcacatcc cgatcacgac cggcgcccgt gtctacttcg
     2881 ccgagtcgat cgaggccgtc cccgagaacc tcaaggaggt tcagccgacg atcttcttcg
     2941 gcgtgccgcg tatctgggag aaattccacg ccggaatcgc ctcgaagctc aaggccgcca
     3001 ccggcgccaa ggccaagctg gttcagtggg cgatgaaggt cggctgggag gccaacaaga
     3061 cgcccgcaag ccacaaagga cttcaatacc gactggcaga caagctcatc ttctcgaagc
     3121 tgaagccggc gatcggcatg gggaacgccc gtgtctgcgt caccggcgcg gcaccgattg
     3181 cgcgcgagat cctcgagttc tttgcgagct tggacctggt gattctcgag gtctacggac
     3241 aatcagagga ctctggcccc acgagcttca acacgccgac ccagtaccgc ttcggcagcg
     3301 tcggcccggc catcccggga gtcgagatca agatcgccga cgacgacgag atcttggtca
     3361 aaggtccgaa cgtgttcatg ggctactaca aagaccccga gaccaccgat gagacgctca
     3421 gcaatggctg gctgcactcc ggcgatttgg gcgccttcga tgacgacggt ttcctcaaca
     3481 tcacgggccg caagaaggac atcatcatca ccgccggcgg caagaacatc acgccgaaga
     3541 atctcgaagc cgcgatcaag aaccacccgc tagtcgacga ggccgtggtc atcggtgacc
     3601 ggcgcaaata cctcagcgcc ctcgtgacga tcgacgccga ggcaggtcct gcatgggcaa
     3661 aggaaaacgg tgaggacccg acggctctcg acaagagcgc caagctgcga gcgagcattc
     3721 aagcgcacat cgacgagatg aacgcgcatt tcgcgcgggt cgagcagatc aagaagttca
     3781 agatccttca ccgcaatctc accgtcgaag acggcgagct cactccgaca ttgaaggtga
     3841 aacgggcgaa ggtgaacgac cacttcgccg aggacatcga gtcgatgtac gtcgaggatg
     3901 cgggaacgca gccacaggcg cgagcctaac caacgaccgt cacggtcacc cgacgctggt
     3961 gaccgcggcg ccgatgctcc caaaggaaga caccctgcca ggtgccgagg gcgcagcgtc
     4021 catccatgac cgggatgctc agcgaagtct gcgtgagtgc ggtcttgacg tgcgcgggca
     4081 tgtcgtccgg accctccatc gtgtggacgt agagcgagtc tccgtcgggg accaagcgcg
     4141 tgagaaacgc ctcgaggtcc cgctgcacgt cccggtcggc gttctcgttg acgatcagcg
     4201 aggcgctggt gtgatgaatg aacaccgtgc agaggccttc gcggatgccg gactccgcaa
     4261 cggcggcgtg tatctgttcc gtgatctcgg tcgttccgcg tccccgcgtc tcgatcgcaa
     4321 tggttttcga tcgcgcgctc atggcgccga taccctcatc cacatggctg cttgatcgcc
     4381 ctggtagatc agaatgtcgt cctgggtaaa acggaactcg tatcgtccga acacctgatt
     4441 ttctcgctgt cggatcttgg tggcgcgtag ctccaagtcg agcggcggcc ccattttcgc
     4501 gagagccgca aagcgcgcgt cgtaaatgcg cccgccgtac ccgatccagc cgtccgcatg
     4561 gcgcaagccg agcacatagt acgtgtgcat gaagccgagg atgcccgtga ggtgcaccat
     4621 gaaacctccg ctgacgtgcc gcgggtgacg gatcgggtgc gctcgctggt cgcgcgtgag
     4681 cggcaagtcg gggtgtgtag acatccgcgc ccgcacgatg cctgcctccg aatcgacctc
     4741 gacgagctcg tcgatgcaaa tcgcaccggg tccatacggg cagtcggcta cgaattccgg
     4801 gtccaatggc atcgtttaca ctccttgggc attcgcatcc cagatcacct tgtgcatttg
     4861 aacctgcacc cgaacgggca agcggtccgc gagaacccat cgcacgagct ccttgggatc
     4921 gagctcgccc caaacgcagc tgagcaggac tgcgtggacg cgttccgcga gatcgtgctc
     4981 ggcgatgacc cctttcgccc aatcgtagtc gcttcgatcg gcgaggacga ccttgacctc
     5041 gtcacgctgc gtaagctgct cgatgttctc ccagcggttg cgatgcgact catcgctgcc
     5101 gggcgccttg agatccatga tgcgatgcac gcgtggatcg acctcagaga tatcgcgctc
     5161 cccgctggtc tcgagaagaa cggtgcgccc cgagtcacag agctctgcaa gcaaggggag
     5221 tgcagcaggc tgaagaagcg gctcaccgcc cgtgagctcg accagcggtg tgccgaacga
     5281 cagcgccttt cggagaacct ctgcccttgg catttcggtt ccaccttcga aggcctgcgg
     5341 cgtatcgcac cagcggcaac gaagattgca gcgggacaag cgaacgaagg tgcaaggaag
     5401 ccccgcgaaa gtcgactcgc cttgaatcga ttcgtagatc tcgtgaatcc gaatctggtc
     5461 gagagcagcc atgttcagcg tgggttgcga gtgatgagga cgagcatctg cccctcttgc
     5521 gccgcgcgct cggggacttc cacgatgaaa tcccacattc ccggctgcgc atccacctcc
     5581 aaggtctcca agcgccgtct aacgcagcgc tccccgtctg gatccacccg aacccagtac
     5641 acgggaaacc tatgaccatc gagatcgaag gcggaagccc ggagcctgct gcgctcctcc
     5701 aactcgaccc gaaagcgata aacccgcgac cccggacccg gagcaccgca acgctcggga
     5761 agagagcccg cgagaggcag tgaggatttg gagacgaggg ccgagaccgg aaagggatcg
     5821 gggaccgcaa cagtgtcagt gccagcgtca gtgccagcgt cagtgtcagc gccagtgcca
     5881 gtgccagcgt cagtgccagt gccagtgcca gcgtcagtgc cagcgtcagc gtcagtgcca
     5941 gcgccagtgc ccgcgaccac ccgccggagc gcgtccagca tctctagcgt gagcaggttc
     6001 ctcacgttgt ttccgtacct gagacctttt ttcgtcaact ggcacgccgc gcgtacgccg
     6061 ccctcgccga gcacgttggg gtgtaccccg tcgcgggcca agcccttgcg gggaagcgcg
     6121 tccagcgccg catgatagtc gatgtaagga agtgaccagt gctcggcgac cgcttccgtg
     6181 atcgcgttga atcggctgat ccagcggtcc ctgttcggat tcctgcgtgg cagggccgag
     6241 ccgagaacgg ggacgacccc catgccttcc agttgctcga tgaggtatac gaggcggcca
     6301 acgtatattc gctcgttttc gttctgtgcg tcgttcccgc cgaatagaac cagggcccag
     6361 cgcgcctgcg tctcttcgag ctccctatgg aatttggcgg ggcggccgcc cagaacgtag
     6421 cgaaggttcc agctcacgcc cgcggcgtga ctctcccgct cgaacgaatt gagctcgccg
     6481 gcattgaagt agtccagggt ccccgaaagc gcttcatgct cgccgagatc gacgtacgga
     6541 gacgcgaagc agtgaatgaa cgctttgctc tccacgctcg agcctcccat cttggagaac
     6601 acgtcatcgc ggagatctgg gttgccagct cggatctcca ggacgtgctc ggccacaggc
     6661 gcatcgagct cgaagggcgc ggcctgagcg ggcccggcga
//
LOCUS       DASRZF010000012        32310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4819433, whole genome shotgun sequence.
ACCESSION   DASRZF010000012 DASRZF010000000
VERSION     DASRZF010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 32310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32310
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4819433"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /gene="purH"
                     /locus_tag="VFG22_00465"
     CDS             <1..679
                     /gene="purH"
                     /locus_tag="VFG22_00465"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235781.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HET6414734.1"
                     /translation="AYRIAREADAQSAFGGIVALNREVDDATAEAVAETFIECIIAPS
                     FAPAALERLQKKKNLRILATGEWLPADYAALQFKRVGGGLVVQDRDATGPSEVRAGKV
                     VTKRQPTDDEMRALDFAWRVCKHVKSNAIIFAKPDRTVGVGAGQMARVTAVGIAAQKA
                     GELSKGAVMASDAYFPFPDGIEAAAKVGISAVVHPGGSKGDDKVIAAADAANMAMVFT
                     GVRHFRH"
     gene            682..1950
                     /gene="purD"
                     /locus_tag="VFG22_00470"
     CDS             682..1950
                     /gene="purD"
                     /locus_tag="VFG22_00470"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HET6414735.1"
                     /translation="MRVLIVGGGAREHALAWRLSQDEGVEVVGAPGNAGLAAIGRTVP
                     LDPTDADRVVALARQESADLVLVGPEAPLVAGVVDALRENGIDAFGPDREAAQLEGSK
                     IFSKEFMARHEIPTAGFAVFDDPDAATRYIEEANRPLVVKADGLAAGKGVIVASDAAE
                     AIAGIDLIMRERAFGSAGDRVLIEECLVGQEVSYHVVSDGKRFIPLAPAQDHKRAFDG
                     DKGPNTGGMGAYSPPPIVTAAVERKIIERVIEPTLAGMAAEGRPFRGALFVGLMIVDG
                     EPLVLEYNTRFGDPECQTLMTRWKGSIMPLIRGSARGDFGGLSPQWEAPASLCVVLAA
                     GGYPGSYEKGKPIRGLEQASALPGVTVFHAGTALEGDQVVTSGGRVLSVTAIGETIDE
                     AAERAYAAAAMIEFEGKQFRRDIGWRARSR"
     gene            1950..2528
                     /locus_tag="VFG22_00475"
     CDS             1950..2528
                     /locus_tag="VFG22_00475"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HET6414736.1"
                     /translation="MEELAPFVKVLEEGGVVACPTETWLGLLADARNPGAVRRVSELK
                     GRPREMPIALLLPNASAVSEVALIPSAGANALMEAHWPGPLTILLLAKPGLSPRLVRD
                     GKVGVRVPGSSPAAELVRAFGHPVTATSANLSGEPPLRSIDELLPSMVLGLGGLVPGV
                     ALGGAPSTLIDSTTTPMQVLRPGAIEIDTAAL"
     gene            2572..2835
                     /locus_tag="VFG22_00480"
     CDS             2572..2835
                     /locus_tag="VFG22_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414737.1"
                     /translation="MALIETEEAARRLARAISSDLALYNEDKIADGIRNDNLFDVMED
                     EIQEGRDLYQSRVAPELIGRNLYDRAIIDLLVRSKAHVESPIW"
     gene            2849..3550
                     /locus_tag="VFG22_00485"
     CDS             2849..3550
                     /locus_tag="VFG22_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414738.1"
                     /translation="MIDTSTGRLALAGCLFLLLAGCGDANTGSAGSAGSGGVAGAGGA
                     SPAPNAGLWYGEDNGFRVCLFVASDGEKLEASPNCDLGNTAQAGEGAYAFDLSVDGVG
                     VDQNGANCSFVARYGGDVPIQALTGVFRVDAAPGTDGSTLSFSGEIVGDQVSGVARST
                     RDASYCEVGWAAVQTAECDDAAINACLDLQDCCRAILINPIFFQSCNSVVLQCDKTAC
                     EQVLAGYPQCAPAEE"
     gene            complement(3559..4503)
                     /locus_tag="VFG22_00490"
     CDS             complement(3559..4503)
                     /locus_tag="VFG22_00490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET6414739.1"
                     /translation="MSDSAPTPTVIILSEEDAGERIDRVLARRCPEYSRSALQRWIEQ
                     GRVEQAGEVVSRKTKAEAGAEVTIDPAPPEPMSAEPQEIPLNVLFEDEHLLVLDKAAG
                     LVVHPAPGHPDGTLVNALLHHAALQGGSDPLRPGIVHRLDKDTSGVMVVAKTASAHER
                     LVEMFQRHELERAYLAIALGRPPASCTFDTFHGRHSASRKKFTSRGDRGRRAVTHLET
                     LELLHGSALVRCRLETGRTHQIRVHLAEHGHPVLGDPLYGKSIADPELRRVSNELGRQ
                     ALHAAVLAFVHPITGEPLRFETEPPDDFQRALSALHLD"
     gene            complement(4507..5124)
                     /locus_tag="VFG22_00495"
     CDS             complement(4507..5124)
                     /locus_tag="VFG22_00495"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HET6414740.1"
                     /translation="MPVSRYWREFDAVEISDTEIAIPGAGTVRRWTRESPEGFVFTVV
                     APKSVSDSGFRRTKENKATCEAVAALAKKLKAKAVVFNADENFKHGKANRSALRSFLG
                     FLPSDFPPVVFDLDAWKPSDIAAACGDRPAVAAYDPLLEDPPPKADFVYIRLPGPAGH
                     RSRYDEASIDKIADHCNSLDPAMGICVFQNIDMQTNAQQLTKRLE"
     gene            5254..6594
                     /locus_tag="VFG22_00500"
     CDS             5254..6594
                     /locus_tag="VFG22_00500"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6414741.1"
                     /translation="MADFGFDAHALDEADTAESPHPLEIARDLIARGKARAALEVLSG
                     HHEEMAHDPDYLLVCSEAWCAEGDELRAQQALLGAARVAPEDPRPLLWLGELLSELGE
                     HEKAERVMAKARALSERPSWTAVSEDHEAPAAEEDLIAFAERQERRAQSALSPKHLAL
                     GVAALTFVGLTVAGIASITSRPEDALVPAEPVAAAPTSALARDATPAIEIDTAVEQSD
                     VEPVLPTPIREAPVELEPAPPPSIGPPAETAATAPEAPPSAPRKAASAKPPKPNPPSR
                     DPGPSASVVQAELASMNPQQLTSRADALYAQGHSGLAASYYRRALELDPDYAPALVGI
                     GRNTLRAKKYSEAMRNATRALELARGVDARPGLEAAALYQMGRVHFEQGERESARRLF
                     RQSISLPGTPPQAWFYLGEALASDNSPVAREAYQRYLELVPRGHLADRARRAIQ"
     gene            6607..6924
                     /locus_tag="VFG22_00505"
     CDS             6607..6924
                     /locus_tag="VFG22_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414742.1"
                     /translation="MVKKPNERRQARKERAREAKEAKREAEERAQQRRRMQLRLSAAV
                     AVLTLLIAGICYWVLENRQLMGITLLIGGVLFLVVALGTLASGVQPRDRTRAGSIDYG
                     NRD"
     gene            6917..7453
                     /locus_tag="VFG22_00510"
     CDS             6917..7453
                     /locus_tag="VFG22_00510"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6414743.1"
                     /translation="MTEEAPLDEFESERWEAAEEGIELLHSGETDRAIDELLRVTRED
                     PTNEYAFHFLGHAYFQKEAFPESLKSYVEALRLAPEYVGAMIGAGQTLRMMGEYDRAI
                     RMGRRVLQKDENDGDGLFLVGAAHFQKGENQAAKRFLERFLQTNPELEIALEVEGMLQ
                     VIRGEVLPFPGAEDTVEN"
     gene            7466..9178
                     /locus_tag="VFG22_00515"
     CDS             7466..9178
                     /locus_tag="VFG22_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HET6414744.1"
                     /translation="MTADPAKKRKVEGSPSSPGDDLRDRARRWREEDVKGAAARHSLR
                     KQRFTTLSDLEVPDLLTPADFDEDYQESLGFPGQFPYTRGVQPTMYRGRLWTMRMFAG
                     FGSPRQTNERFRYLLEQGQTGLSTAFDFPTLMGYDSDSPRSLGEVGMVGVAVDTLHDM
                     EILFDQIPLDKVTTSMTINGPAAVLLGFYVALADKRGVPRDRIGGTVQNDCLKEFIAQ
                     HAWVVPPRPAMRIVTDSIEFCAKEVPRWNSVSISGYHIREAGSTAAQELAFTIADGLE
                     YVNWGVERGLDVDAFAPRLSFFFDVHSDFFEEVAKFRAARRMWARLMKERFNPKDPRS
                     MMLRTHAQTAGVSLTAQQPYNNVARVALQALAAVLGGTQSLHTNSLDETFALPTEEAV
                     TVALRTQQIIAEESGVTNTIDPLAGSYFVEWMTNRMEEEAMGYIRKIDELGGMVEAVE
                     RGYPQREIAASAYRMQQQIESGEKTLVGVNKYVQDEQPDIPTLRVDESIQKEQVESLA
                     RVKAQRDAKAVSKALAAIAEDCRADRNVMPGIVEAAKVYCTEQEICDVFREVFGQHQD
                     RPEF"
     gene            complement(9181..9594)
                     /locus_tag="VFG22_00520"
     CDS             complement(9181..9594)
                     /locus_tag="VFG22_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414745.1"
                     /translation="MTHPDEDPELPGKPRRRPSHFRKKRLDTTTKVVRVLRERSATPS
                     DPGHVVQSRRSRQEQWRDVINPAPALPDPLLQRPLPPPAPLPSEKSSLPDLAAPGWLD
                     DRHQRKRVEDLIGTVGLALMVAAMLVAMWLRMTGN"
     gene            9706..10416
                     /locus_tag="VFG22_00525"
     CDS             9706..10416
                     /locus_tag="VFG22_00525"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HET6414746.1"
                     /translation="MDTQQAAEEIDGDFWGRLVFTSEHASAELPEPWKWPADDRWLID
                     THWASDLGAAAFTRRVARLMNAPAVLSRFSRLLIDPNRPLDSETLFRENADGKTVHLN
                     EGLLEAERQRRIDQFYHPYHAAVRSMVKKSRGDTVFSIHTFTDNYEGEARTLEVGVLF
                     DHDEEPAQRLVRHLRGAGFHAAPNEPWSGKLGLAYSPVLHAREYGRCALEIEARQDLI
                     VQESFAQRLAEALAGFFA"
     gene            10427..11281
                     /locus_tag="VFG22_00530"
     CDS             10427..11281
                     /locus_tag="VFG22_00530"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6414747.1"
                     /translation="MPHMRSERTGMSYLSSGDEGAPLVLCLHGFPDIPRTWAPLTDRL
                     VPAGYRVIRPWLPGYAPSSLEGPFDVPSLARKMLSLIDELSPSEPIRIVGHDWGAILT
                     QLVTAQWPERFRASAALAVPHMLAFEANVERYPRQLLKSAYMGFFQIPGLSDRALKLR
                     NYRLIERLWASWSPGFDPGDAHFEELKACLRSSMPAPLRYYRALKSPKAIREIRAIVG
                     AGPIVVPTLYLHGERDGCIGPELADGQEQHYSALFETVKLAEAGHCLHLERPAEVNAA
                     ILRWFEAH"
     gene            complement(11305..12756)
                     /gene="purF"
                     /locus_tag="VFG22_00535"
     CDS             complement(11305..12756)
                     /gene="purF"
                     /locus_tag="VFG22_00535"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HET6414748.1"
                     /translation="MDAWVLRRYENPVWDPDDDHFHDQCGVFGVFGADEASNLAYLGL
                     HSLQHRGQEAAGIVSSNGAQLFVHRGMGLVQDVFTADQIDKLPGSSAIGHVRYTTAGG
                     SHIKNAQPLAVDCGHGSIAVAHNGNLTNGLALRHQLEKDGSIFSSDSDTEVLVHLIAR
                     SKAETMIDRVADALHQVEGAYSLVFLSPDELVAVRDPYGFRPLCLGKLDGAYVIASEP
                     PAFQLIGAKFIRDINPGEMVVINETGLRSLRPFRTDAERRYMCIFEYVYFARPDASFD
                     GISVYDARKRMGRALWEDQPVEADLVVPVPDSGVAAALGYAEAAGLPFELGLIRSHYV
                     GRTFIEPAQSIRHFGVRLKLHPVQSLLEGKRVVVIDDSIVRGTTSRKIVKMIRDSGAK
                     EVHLRISSPPTIWPCFYGIDTPTREELIAAHHSVAEVNDYVTADSLGYLSLEGLKRAV
                     GGEGFCSACFSGRYPIPVTDDINLRRQLPLVGV"
     gene            12807..15446
                     /locus_tag="VFG22_00540"
     CDS             12807..15446
                     /locus_tag="VFG22_00540"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HET6414749.1"
                     /translation="MPRILKRIESVVPRRASSYGGKARGLAALARAGFPVPAAYAMPG
                     WVGDSFFSSVLEISDRPRALLRTPHVPDARLQTIAERVRAATLPQDVVRSVRDAVLAM
                     QDEGATSFAVRSSAMHEDEEGASAAGMHLTMLNLIREDEILDAIKACWASTFRPRVLS
                     YLRALGEDVPVSVGIVIQAMVPSEISGVLFTANPLTGDAGEVVINAAYGLGSSVVDGR
                     VTPDTFRVDKATGQLRDRIIGDKAQQTLLERSGGVREVAVDPADRERASLSEPQLLHL
                     SELADRIERHFGDARDVEWAIAAQQVYVLQARPIVVSEVRSRRGASRERPVDRRKIVW
                     SNANIGEALPGVATPFTWSVLSQFSDLGFRRAFGAMGCTVPRDAELVGDFRGRIYLNL
                     TEFSAIMAQIPWIHPSTLMRLGGGQYASELDEVIADRSSTGFFLRLPQTVSRYTRENF
                     RFKARVEDFEAYFSDERMRMSGTDPRLLEPSGLDRMLGDVEHLLDSTGSIMLTAYANL
                     LTTVLLLIGLLRAFAQDLGPGFYRELLSGLQDVASARPGFVLRQIAQLAREEPELVAL
                     IRDADLSELQISNLPAGRTRDALRRFLDDYGYRGVREAEIAAPRWSEDPTLMFASIRS
                     HLANTVDLDVRERDLQQRRADAERRLEKNVPFVIRPAVNKLLDLVRRYTRMREHLRAH
                     VVEVLGMFRRVALEASRRIEAREPEAGPDSAFFLTLTEVRRVLYDENESVAIRVQRRR
                     LEYARNQALPEPPTTFVGFPPDDLGVATATKRLRGLAASRGIVEGRVRLLSKPSQAAD
                     FRTGEILVVAAADTGWAPLFLAASGVITELGGPLSHAAIILREYGVPAVVNVTNATRS
                     LCDGDLVRLDGDAGTVEILSGSS"
     gene            15433..16152
                     /locus_tag="VFG22_00545"
     CDS             15433..16152
                     /locus_tag="VFG22_00545"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET6414750.1"
                     /translation="MARAEAVEPEIIHHDADLLVLHKPPGLPTSAPVASDPSLVRWVS
                     ERFPELRAHATSRLDSQVSGVVTFALNREANQHLLDARKSGTYERIYLGITVHELADD
                     AGRWDWPISIDPRDPKHRRAGAGRGERHAMTRYEVAARRSGATLLRLMPATGRTHQLR
                     VHAAEAGAPLFGDPAYGGERRAVLPDGAVVTARRVMLHCARVSFPSLSGPERIVIEAA
                     VRPDMQRVWQAFGGAASELQP"
     gene            complement(16143..17177)
                     /locus_tag="VFG22_00550"
     CDS             complement(16143..17177)
                     /locus_tag="VFG22_00550"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6414751.1"
                     /translation="MSEQNEGFMDRLSAHLDRGWELVTQGDLNGAMASAEQTLELDGD
                     SPDAHNLIGYIHAANGNLDLALEHYRQAIDLDEHYLEAMLNAAELLIHPLGSFAEAIR
                     VLDQALESCQTADDIADASVLKVDALLQQGDHAAAKELLLGLPEGPFENQQLDFLIGR
                     AHFELDQIEEAAALIERAAEPSDASPDILYYLGLLRDRQERPDEASLAFLKTRHLDGL
                     LPRAPWSPSHARFEKIVQSALRELPEELAQRLEGNLIMVTDLPGLEVVAEGVDPRTPL
                     LLDEMATNAGGEEQRRLFVYQRNIERLIRHPLGLQEDVQEILHRELEAHFNRPVDVPP
                     GTKFTTKRQG"
     gene            17246..18130
                     /locus_tag="VFG22_00555"
     CDS             17246..18130
                     /locus_tag="VFG22_00555"
                     /inference="COORDINATES: protein motif:HMM:NF015376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HET6414752.1"
                     /translation="MVRFFTALGIVAICSCVNEPLPVGQAISVGTAEHGYLHDAVRLE
                     DRGDGYRRIRPGEGSRYGTPTLVGAIERAAQEVARAFPGGNPLRVGDLSNPHGGQHSR
                     HRTHRAGRDADLLFFAEDGGGVAAPSDGWPEFDRFGLSALGGRVFGFDEARNWHLVRT
                     LVMDERARVKWLFCSNELKARLLRYAARHEHSPRAILRATWILHQPSAGDSHRDHFHL
                     RVGCGEFERHFGCEEQAPHWPWLSDPARKEDIRANPAASDEHLVRWLFAEEHVDERRD
                     VIWRRLDAAAQPSTVARR"
     gene            complement(18038..18811)
                     /locus_tag="VFG22_00560"
     CDS             complement(18038..18811)
                     /locus_tag="VFG22_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414753.1"
                     /translation="MQRETPMAQMVREAIETVATPEVRLRILHRALHMAREHEIPTTG
                     EPLHRFIDKHLRTAMAFYVGDEASEAVMQNLAPMLTMADMVNSQPPRQTVRADATRKL
                     GRDRQDSGVTRRAPGPAPETDTSKYPTMQPQGSALPMVFVATRSRERCARIAKQVGSS
                     AAVQQIEDVVAFLDNLKATASLSPLVVIDCVEASVQPTTIATLAHELPKSSAVLIWGA
                     TERHRDLTDLATSDRGWLRCGVEATPDDVASLIHMLLGE"
     gene            18930..19862
                     /locus_tag="VFG22_00565"
     CDS             18930..19862
                     /locus_tag="VFG22_00565"
                     /inference="COORDINATES: protein motif:HMM:NF013514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414754.1"
                     /translation="MADMLVMGVDENGLGPRLGPMVATALTLETPRYARAFLTKRGLA
                     LGLTDSKQTGGFGRLGFTESAALGVLRLAGLGTPGSADELLERVSPGTSEHIRCRCPD
                     DSSAAQCWAVDFPLPGFGGDASFGVDLLERLVGRSRLRIVDVQSRIICAGLLNERLAA
                     GQNKLAVDLELFEQLIASVQSRHGSPLLAVCGMIGGIRDYQSRFSRFEAGRVKELRRR
                     RGQRRYSIDRLGEVRFEVDADARHLPVALASIVGKYLREICMRRIGEFYRRDDPALEL
                     SSGYHDPVTTRFIDATEPARRRLQIAPDCFRRQA"
     gene            19884..21029
                     /locus_tag="VFG22_00570"
     CDS             19884..21029
                     /locus_tag="VFG22_00570"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET6414755.1"
                     /translation="MSERDRIPRWALWLGALLLFGWLLYTLRGVLGPVFFAFLIAYML
                     DPLVDRIEQSRLLRGRAIGRGVGIAVLLAGLFAASAFAILVVAPMVYEEVSSFVLRLP
                     GLVARSRAEWEPALAELGLRVPASVGEAFEELHLDMQSVLTKGYTPATAFAKSLLGGT
                     ASALAAIIASLMVPVFAFYLLYDFDGLVAKAGELIPPRLRPQTNSFFADVDAVLGQFF
                     RGQFTVMAILSALYSLGYGVIGVPLALPIGLMAGFLSFIPYVGSSLALATALLMTALD
                     WQGWTQIFWVLGVHAIIQGLEGFVITPKIMGDTVGISAIAVLLSLLVGAELLGFTGVL
                     LAVPAAAVTKILVTRIDQRYRQSEFFAGQKPDANEVTDHSKDESRRA"
     gene            complement(20992..21927)
                     /locus_tag="VFG22_00575"
     CDS             complement(20992..21927)
                     /locus_tag="VFG22_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414756.1"
                     /translation="MSRSLKVLALVSIMMAALLFAANRHGRSLRARRSLEPFPNTARA
                     LLRIDMRALERTEAARILLKAFMPDDALSEIETACGFDPLASLSDATVWVRGPDEEPF
                     QAIGLMLQGSSADGEALAACHRLLVERRGGSVLRLDSPAGPLISSRDGQSAIALLDER
                     TIVTGSVRTVTEIMEVRRGTASSLLERATIARLWPRLSIGAAIAAIVEPPAHWKSALA
                     GAARFGSDASPLDDAQAIAFSVKSGSDDTLELYIEVSGEVQAQKNAELIEAWIATPPD
                     TFEPPWLALLQSARVQRRAATIMVKLDVTHLSSDR"
     gene            21999..22679
                     /locus_tag="VFG22_00580"
     CDS             21999..22679
                     /locus_tag="VFG22_00580"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H"
                     /protein_id="HET6414757.1"
                     /translation="MRLRNAEVLARCDESGDLVANGGRVEVRYKPNDGRAYFAAASNL
                     TPPAGALQIEPDSYCGPGESVQKQSKPSKKSAASASAPESPQGDELLVYADGACSGNP
                     GPAGVGVVALWGDQRRELSEYIGEATNNIAELTGILRAAELAQELGRPLRLYTDSKYS
                     IGVLTQGWKAKANKELVAAVRAALQAQRDAKLFHVRGHQGVRLNEHADELAVLAVQNR
                     KSTGWVDR"
     gene            complement(22657..23592)
                     /locus_tag="VFG22_00585"
     CDS             complement(22657..23592)
                     /locus_tag="VFG22_00585"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET6414758.1"
                     /translation="MTLKLASISIDLDETPRYAEIHGLEQLRGPAAHAVYDAAVPRLE
                     AWLEEESIPATFFVIGEDLERDENRASVARLHRAGHEIGNHSYHHHYDLTRRAAVEVR
                     EEIERGAAIIERACGTRPSGFRAPGYTVSDALFSALDVAGVEYDSSVFPCPSYYAAKA
                     LALSAFRAIGRSSASVLDTPAVMRAPRQPYRVGRPYWRRGDGILELPIGVTPLQLPFI
                     GTSLVLGGKRMAERLSKQMLGKELINLELHGLDASDAIEDGLETLALHRADLKRGATE
                     KLGVIRAAVRVIRDAGYELVTLAEAARRFSDRPSP"
     gene            complement(23589..24311)
                     /locus_tag="VFG22_00590"
     CDS             complement(23589..24311)
                     /locus_tag="VFG22_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET6414759.1"
                     /translation="MTESNAPPRISIVIPVYNEEAILRAALIDLLDRLSALPWPYELI
                     VAENGSTDRTFEEALALAERFEQLVCRHTGEPNYGSALRDGILHAKGEIVICDEIDLC
                     DTDFYQRAMALLEDRAVDFVVGSKVMEGAMDQRPRFRRLGTIVINGMLRLALGFKGTD
                     THGLKAFRRKPVDPIARACLVDRDLFASELVIRAERAGLCVREIPIRVLEKRPPTVGL
                     VKRVPNVLKNLAKLFIAIRIKG"
     gene            complement(24345..25415)
                     /locus_tag="VFG22_00595"
     CDS             complement(24345..25415)
                     /locus_tag="VFG22_00595"
                     /inference="COORDINATES: protein motif:HMM:NF042587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HET6414760.1"
                     /translation="MWDKVGPKNWRRPLLLQPDNFTPPSRTPWGGRRITGRLKANAGL
                     QDRGPVGEAWELSVEPDFPSRLVDGPTLDEVLRADPALLGVEAELGSTALLVKLVDAA
                     DDLSVQIHPRDDDPKLAADESGKPEAWYIIEADAGAGLYLGFRDGVSRQEVETAIDEE
                     GAVDALMSFVPVSPGDVFLIEPGTPHAIGKGVLLLEPQRVAPGRRGLTYRYWDWNRKY
                     DSAGRLDPEGKPRALHRERALDVTRWDRPRGAALLSEVRHRSGPARTRDTARLEILAS
                     KTGPARSEQFSLRRLTGTGHLVLIAENRLRSLTVLAGRARLDDGATQTILSSGQTAAL
                     PACLGDLQIDLEAAHGVLAALA"
     gene            25461..25778
                     /locus_tag="VFG22_00600"
     CDS             25461..25778
                     /locus_tag="VFG22_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414761.1"
                     /translation="MGYLGKERRIHRIFVTRNSEYHVRRNVCVGVRDRRSGEWLGGHL
                     AVRSTVSGGLKFHDNGAISASEGLPTVGESLFFIAAGRDLITSPVLNVERPAREIVHN
                     YPI"
     gene            25812..26213
                     /locus_tag="VFG22_00605"
     CDS             25812..26213
                     /locus_tag="VFG22_00605"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HET6414762.1"
                     /translation="MSSEMKTIRVVAALVERDGRYLITQRRESAVLPSLWDFPGGRVE
                     DGETDEAALAREVSERLGATVEVGQLISFVNHPYEKYAVDLYLYQCELLSDRLHCRAV
                     KDYAWVTSKEMESYAFTPVDEASMSKLLGEG"
     gene            complement(26223..27278)
                     /locus_tag="VFG22_00610"
     CDS             complement(26223..27278)
                     /locus_tag="VFG22_00610"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6414763.1"
                     /translation="MAYSSAAEARQARELLGKALEALQKDSEVPEEVLGLTQNVAKSI
                     GALFEAEYASTEVDGKTCVRNALGTLSQTLALLQDVKSEHAGVETATESIADVISILF
                     PLTNKPSVRPELTPLASTAPQSRPPQSTRPRSAPPQSTPPQSTRPLPGSPQSAPPQSV
                     PPSSRPPASAPPVRVAPSILPPASVPPSIRPPSSRPPSSRPPSSRPPLSHRPSERPQS
                     GRPRSGAPQPIPTSLLPPPPPIPKGERQAVEANLGATTQSNFYVGFSGEIAHGGVFLA
                     TYEALPKDTSVRMLVTLPGGFEFECEGYVRFVRDPMDFMSESEPGMGIQFENLSDEAR
                     NLVLRFIRQRPPIFYDV"
     gene            complement(27334..27771)
                     /locus_tag="VFG22_00615"
     CDS             complement(27334..27771)
                     /locus_tag="VFG22_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6414764.1"
                     /translation="MEIRGITKVDYDYIISVLDQWWGGPAGKRADPMFFYEFGDHALM
                     AERDGQLIGFLFGVMVPAPSATGYVHLVGIHPDHRRRSVGKHLYEHFSERCRAAGMKR
                     IKSIASAGHEGPVRFHQSMGFESNEVADYAGPGRSRVVFVKEL"
     gene            27831..28412
                     /locus_tag="VFG22_00620"
     CDS             27831..28412
                     /locus_tag="VFG22_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414765.1"
                     /translation="MPPSPASSERVEILFCPFCGDGFEDRSDCPDHDLALLPLDELPR
                     SGQRQLQGVTFFADPRLGRGPVLLGISLALLGFLVPMVRYGDVVESALEVAIEGAGNL
                     WLAPGAGLAVLMILWLRRSRSAMSGARAAVFGLALGGILPLLYTGRRIGMLAESFSTS
                     LEWQWGLWLMIAGFVAAALGSVRLGGPRLRPES"
     gene            complement(28390..29364)
                     /locus_tag="VFG22_00625"
     CDS             complement(28390..29364)
                     /locus_tag="VFG22_00625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6414766.1"
                     /translation="MGTAGPSSKAVVVVADDDRATRDHISQVLQTRGFAVVLTSSGQK
                     AIDAVRAQPVDLVLLDVDMLGLSGVDACRVIKSITKDRFVPVVLLTAPGDLSARVQGL
                     RTGADDHIAKPLDDEELLARIENMLRVKRTRDDVQFAKRELNYASLHDQLRSMPDHRY
                     FHERLESEFDEAQRHLDPLACCIVAVDDFRALVTERGADLAARMLDEVSERLQRTIRA
                     TDIAARFRTAEFGLLLPNTRPARALTLADRIVSEVAFRPFELNGATFEVTVAIGVGLY
                     PSGSIRSHNELLDAASIAVARARVAGQNRVCVVQQQGYIFRPTIPGAA"
     gene            29481..30902
                     /gene="rpoN"
                     /locus_tag="VFG22_00630"
     CDS             29481..30902
                     /gene="rpoN"
                     /locus_tag="VFG22_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HET6414767.1"
                     /translation="MELKQQLKLSQQLIMTPQLQQAIKLLQMSRMELTELVQEELLEN
                     PILEEGMEARDSTSAGTDEAPSAEVKAHEQKKDEIDWERYLENQSNEAPMPSIRRGTD
                     EEMPGIEATVSSAENLDDHLGWQVRMGDFVEDERRFASLVIGNLDDNGYLKLEGLAPE
                     EVVPRLAAEADLDPEDAEEVLAMIQQFDPIGVASRSLEECLLIQAKHYGMDELVLRII
                     GDHLGDLEKRHYAAIAKVCGCALDEVYDVAQIIAELEPRPARRFQTEEPRYIIPDVYV
                     HRVGDEYYVVANDDGMPKLKISGFYRSAMADNPKAKEYIQDKLRSAQWLIRSIDQRRK
                     TIVKVTECIVEKQHDFFDKGIEHLKPMILRDVAEAVGMHESTISRVTSNKYVHTHRGT
                     FELKYFFNSAIRRDHQNDIASESVKQAIKGIIGGEDPKAPLSDQRIVEILAQDDIKIA
                     RRTVAKYREMLGILSSSKRKKYF"
     gene            30917..31243
                     /gene="raiA"
                     /locus_tag="VFG22_00635"
     CDS             30917..31243
                     /gene="raiA"
                     /locus_tag="VFG22_00635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HET6414768.1"
                     /translation="MRISYTFRNMESSDAIKSHASEKIGKIQKYMRAPLHAEFTFSME
                     RHLHRVDLNLTGDGHQYSGHGESEDMYTTIDQVVDKIDRQVRDTKASESDRRRHGGET
                     LRRSED"
     gene            31267..31734
                     /locus_tag="VFG22_00640"
     CDS             31267..31734
                     /locus_tag="VFG22_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET6414769.1"
                     /translation="MGYRLAFMLADLLHPERVRTGLILPDKEAALRAVASLLTMPEGE
                     VNEEVVYDVLVDRERLASTGIGSAVAIPHGRYDGMEELRAAVAICPGGVDFDAVDQLP
                     VKILVGIIGPKSMPQKHLAALAGVSRLLRMDAKREQLLRAVDRTEAYRALTES"
     gene            31774..>32310
                     /locus_tag="VFG22_00645"
     CDS             31774..>32310
                     /locus_tag="VFG22_00645"
                     /inference="COORDINATES: protein motif:HMM:NF014642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HET6414770.1"
                     /translation="MTESSSSRQGGLASMRVDRGITVARLLAEPRLKPILRLVAGQRG
                     EERAIDHPRVQKPGLALAGHTHGVVPTRVQILGETEITYLDTLSPDEQLKRVTQLFDL
                     GLSLVVVTRGVEPPAALIQAAQETGTPLAVAAPRSSRAIQAIHLVLDGILAPSQTRHG
                     VLMDVHGIGTLLLGPSGIG"
BASE COUNT         5721 a        10022 c        10481 g         6086 t
ORIGIN      
        1 agcgtaccgg atcgctcgcg aggcggacgc gcagagcgcc ttcggcggaa tcgtggccct
       61 caaccgagag gtcgacgatg cgacggcaga ggcagtcgcc gagaccttca tcgagtgcat
      121 catcgctccg agcttcgcgc cggccgctct cgagcggctc caaaagaaga agaacctccg
      181 gattctcgcg accggggaat ggcttccggc ggactacgcg gcgctgcagt tcaagcgcgt
      241 cggcggcggt ctcgtcgtgc aggatcgcga tgcgaccggc ccgagcgagg ttcgcgcggg
      301 caaagtcgtg accaagcgtc agccgaccga cgatgagatg cgcgcgctcg atttcgcctg
      361 gcgcgtttgc aagcacgtga agtcgaacgc gatcatcttc gccaagcccg accgcactgt
      421 gggtgtcgga gccgggcaga tggcacgagt cactgccgtc ggaattgcgg cgcagaaggc
      481 gggcgagctg tcgaagggtg ccgtcatggc gtcggatgcg tacttcccgt tccccgatgg
      541 catcgaagcc gcagccaagg tgggcatcag cgcggtcgtc catcccggtg gctccaaggg
      601 cgacgacaag gtcatcgccg ctgccgatgc agccaacatg gcgatggtct tcaccggcgt
      661 tcgccacttc cggcactgag gatgcgcgtt ctcatcgttg gaggcggggc ccgcgagcat
      721 gcactggcct ggcggctctc gcaggacgag ggcgtcgagg tcgtcggagc gccggggaac
      781 gccggcctcg cggcgattgg acgcacggtt cctctcgacc ctaccgacgc cgatcgtgtg
      841 gtggcgctcg cgcgtcaaga atcagctgac ctggtgctgg tcggcccgga ggcgccgctg
      901 gtcgctggcg tcgtcgatgc gctccgcgaa aatggtatcg acgccttcgg cccggaccgg
      961 gaggccgccc agctcgaggg ctccaagatc ttctcgaagg agttcatggc gcgccacgag
     1021 atcccgactg cgggatttgc cgtcttcgac gatccggacg cggcgacccg ctacatcgag
     1081 gaagcgaatc gtccactggt ggtgaaagcc gacggtctcg cggcgggcaa gggcgtcatc
     1141 gtggcgagcg atgccgccga agcgatcgcc gggatcgacc tcatcatgcg cgaacgcgca
     1201 tttggcagcg caggcgatcg agttctcatc gaggagtgcc tcgtgggtca ggaggtgagt
     1261 tatcacgtcg tttccgacgg gaagcgcttc atcccactgg ctcctgcgca ggaccacaag
     1321 cgtgcctttg acggtgacaa gggtccgaac accggaggca tgggcgcgta ctctccacct
     1381 ccgatcgtca ctgctgccgt cgagcgaaag atcatcgagc gcgtgatcga gccgactctc
     1441 gcgggtatgg cggctgaagg gcgcccgttc cgaggcgcgc ttttcgtcgg cttgatgatc
     1501 gtggacggtg agcccctggt gctcgagtac aacacccgat ttggtgatcc cgagtgccag
     1561 accttgatga cgcgctggaa ggggtcgatc atgcctttga ttcgcggctc tgcgcggggc
     1621 gatttcgggg gattgagccc tcagtgggag gctcccgcct cgctttgcgt cgtcctggcc
     1681 gctggcggct accccggcag ctatgaaaaa ggcaagccga tccggggcct cgagcaagcg
     1741 tctgctctcc cgggcgtgac cgtgtttcac gcgggcaccg cgctcgaagg agaccaggtc
     1801 gtcacctcgg ggggaagggt cctttcggtg acggcgatcg gcgaaacgat cgacgaggcc
     1861 gccgagcgag cgtacgcagc cgcggccatg atcgagttcg aaggcaagca attccgccgt
     1921 gacatcggct ggcgggcgcg aagccgctga tggaagagct cgctcccttc gtgaaagttc
     1981 tcgaggaggg cggggtcgtg gcctgtccga cggagacctg gctgggcctc ctggccgatg
     2041 cccgcaaccc cggggccgtc cgacgggtct ccgagctcaa gggccggccg cgcgagatgc
     2101 ccatcgcctt gctccttcca aacgcttcgg cggtttcgga ggtcgctttg atcccctcgg
     2161 ccggcgccaa tgccttaatg gaggcgcact ggcccgggcc tttgacgatc cttttgctgg
     2221 ccaagcccgg tctgagccct cggctcgtga gagacggcaa ggtcggtgtc cgcgttcctg
     2281 gttcgagccc ggcggccgag ctggtgcgcg cgtttgggca tccggtcacc gccacgagcg
     2341 ccaatctgag cggggagccg ccactccgga gcatcgacga gctgcttccg agtatggtcc
     2401 ttgggctggg cggattggtt ccgggggtcg cactgggggg cgcgccttcg accttgatcg
     2461 actccaccac gacgccgatg caggtccttc ggcccggagc gatcgagatc gacaccgccg
     2521 ctctttgaca gaggcggtgc tgctgcgtca ctctgcgcct acggaggacc gatggcgctg
     2581 atcgaaaccg aagaagccgc acgtcgcttg gcacgcgcaa tctcgagtga ccttgctctc
     2641 tacaacgagg acaagatcgc cgatggcatt agaaacgaca atcttttcga cgtgatggag
     2701 gacgaaattc aagaaggtcg tgatctttat cagagccgag tcgcgcccga gctcatcggc
     2761 cgcaacctct acgaccgagc gatcatcgac ctgctcgttc gctccaaggc gcacgtcgaa
     2821 tctccgatct ggtaagcctg ggttcgcgat gatcgacact tcgaccgggc ggctggcgtt
     2881 ggctggctgc ctttttctcc ttcttgctgg gtgcggggat gcgaacactg gctcggcggg
     2941 cagcgctggc agcgggggcg tcgccggcgc tggtggcgcg agcccggcgc ccaacgcggg
     3001 tctttggtat ggagaggaca acgggtttcg cgtctgtctc ttcgtcgcct ccgacggcga
     3061 gaagctcgag gcgagcccca attgcgacct cgggaacaca gcgcaggccg gcgaaggcgc
     3121 ctacgccttc gatctcagtg tcgatggggt cggagtcgat cagaacggcg cgaattgcag
     3181 cttcgtcgcg cgctacggcg gcgacgtgcc gatccaggcg ctgacggggg tgtttcgcgt
     3241 ggatgcagct cctggaacgg acggctccac gctctccttc tcgggcgaaa tcgtggggga
     3301 tcaggtctcg ggcgttgcgc gctccactcg ggatgcttcc tattgcgagg tcggatgggc
     3361 ggccgtgcag accgcggaat gcgacgatgc ggcgatcaat gcgtgcctcg acctccagga
     3421 ctgctgcagg gcgattctca tcaatccgat cttcttccag tcctgcaatt ccgtcgtgct
     3481 tcaatgtgac aagactgcgt gcgagcaggt gctggccggg tacccgcagt gcgcgccggc
     3541 cgaagaatag tcagcgctct aatcgaggtg caacgcgctc agcgcgcgct gaaaatcgtc
     3601 cgggggctcc gtttcgaagc gcagaggctc gcctgtgatc gggtgcacga aggcgagcac
     3661 cgcggcatgc agcgcttgcc gacctagctc gttcgaaacg cgccgaagct cggggtcagc
     3721 gatcgacttc ccatagagcg gatccccgag aacaggatgg ccgtgctctg ccaggtgcac
     3781 gcggatctga tgcgtgcggc ccgtctctag acgacaacga accaaagcac ttccgtgcaa
     3841 aagctcgaga gtttctagat gtgtgaccgc ccgccggccc cggtcaccgc gtgaggtgaa
     3901 cttttttcga ctcgccgagt gtcgcccatg gaaggtgtcg aaggtgcacg acgccggtgg
     3961 gcggccgagc gcgatcgcga ggtacgcgcg ctcgagctcg tgcctctgaa acatctctac
     4021 caggcgctca tgggcgctcg ccgtcttggc cacgaccatc actccgctgg tgtccttgtc
     4081 gagccgatgc acgatgcctg ggcggagcgg gtcagagccc ccctggagcg ccgcgtgatg
     4141 caatagcgcg ttcaccagcg tgccatcggg gtggccgggt gcggggtgga caaccaggcc
     4201 tgcggccttg tcgagcacga gcagatgctc gtcttcgaaa agcacgttca gcgggatctc
     4261 ttgcggctcc gcgctcatgg gctcgggcgg ggcggggtcg atcgtcacct ctgcgccggc
     4321 ctcggccttg gtctttcgtg aaacgacctc gccagcctgc tcgacgcggc cttgttcgat
     4381 ccatcgctga agggcgctcc tcgaatactc cggacaacgg cgtgccagga cccggtcgat
     4441 gcgctctccg gcatcctctt cagagaggat gatcaccgtc ggcgtcggcg ccgagtccga
     4501 caccgcctac tcgagtctct tggtgagctg ctgggcgttg gtctgcatgt cgatattctg
     4561 aaacacgcag atccccatcg ccgggtcgag actgttgcag tggtcggcga tcttgtcgat
     4621 tgaagcctcg tcgtagcggg accggtggcc tgccggcccg gggagacgga tgtagacgaa
     4681 gtccgccttt ggcggaggat cctcgaggag tggatcgtag gctgcgacag ccggccggtc
     4741 tccgcaggcc gccgcaatgt cacttggctt ccaggcgtcg agatcgaaga cgaccggtgg
     4801 aaagtccgat ggcagaaaac ctagaaacga tcgaagagcg ctccggttgg ccttgccatg
     4861 cttgaagttt tcgtccgcat tgaagacgac cgctttggcc ttgagcttct tggccagggc
     4921 cgcgacggcc tcgcaggtcg ccttgttttc cttggttcgc cggaaccccg agtcgctcac
     4981 cgatttgggt gccacgaccg tgaatacgaa gccttcgggg gattcccgcg tccagcgccg
     5041 gaccgtgccc gcacctggaa tggcgatctc ggtatccgag atctccaccg catcaaattc
     5101 ccgccagtat cggctgaccg gcacggggaa gccggagcac gcgaccacaa acattgcgcg
     5161 agagtataca agctttggcg cagaaggccc gaggtgactc ctcgattggc gggaattcct
     5221 cggttccgga tcggctctga taggatcgtg gccgtggctg atttcggatt cgacgcccat
     5281 gcgctcgacg aggcggatac cgccgagagc ccgcatccgt tggagatcgc tcgcgatctg
     5341 atcgcgcgcg gaaaggcccg cgccgcgctc gaggtgctga gtgggcatca cgaggaaatg
     5401 gcgcacgatc cggactacct gctggtctgt tccgaggcct ggtgtgccga aggcgatgag
     5461 cttcgggcgc agcaagcgct cttgggcgcc gcgagggtgg cgccagagga tccgcgaccc
     5521 ttgctatggc tgggcgagct cttgagcgag ctcggggagc acgaaaaggc cgaacgcgtc
     5581 atggcgaaag cgcgcgcctt gagcgagagg ccgagctgga cggcggtttc cgaggaccat
     5641 gaggcgcccg ctgcggaaga ggatctgatc gcgttcgcag agaggcagga gcgcagggct
     5701 caatctgccc tcagcccgaa gcatctcgct cttggcgtcg ccgcattgac cttcgtcggc
     5761 ctgacggtgg ctggcatcgc gagcatcacc tcgcgtccag aagacgcctt ggtccccgca
     5821 gagcccgtcg cggccgctcc gacgagcgcg ctcgcgcgag atgcaacccc agcgatcgag
     5881 atcgacaccg ccgtcgagca gagcgacgtc gagcccgtgc tgccgacacc gatccgagag
     5941 gcgccggtgg agctcgagcc ggcaccccct ccgtcgatcg gtccaccggc cgagaccgcc
     6001 gcaaccgccc ccgaggctcc gccgtcggcg ccacgcaagg ccgcgtccgc gaagccaccc
     6061 aagcccaatc caccttcgag agacccgggg cccagcgcga gcgtcgtgca agccgagttg
     6121 gcatccatga atccgcagca gctcacgtcg cgcgccgatg cgctctacgc ccaaggacac
     6181 tcgggccttg ccgcgagcta ctaccggcgc gcgctcgagc tcgaccctga ctacgcgccc
     6241 gctctggttg gaatcggccg aaacaccctg cgcgcaaaga agtactccga ggccatgcgc
     6301 aacgcgacgc gggcactcga gctcgctcgt ggggtggacg cgcgtccggg cctcgaagct
     6361 gcagccctct atcaaatggg ccgcgtccac ttcgagcagg gcgaacgcga gtcggcgcgg
     6421 cgcttgttta ggcagtcgat ctcgcttccc ggcacgcctc cacaggcctg gttctacctc
     6481 ggcgaagcgc ttgcgagcga caactcgccc gtggccagag aggcctacca acggtatctc
     6541 gagctcgtcc ctcgggggca tttggcagat cgggcccgca gagcgattca gtagtatgct
     6601 ccgacaatgg tgaagaaacc gaacgagcgg cgccaagctc gaaaagagcg cgcgcgggaa
     6661 gccaaggaag ccaagcgcga agctgaggaa cgcgcgcagc agcggcgccg catgcagcta
     6721 cggctgtccg cagcggtcgc cgtcctcact ttgctgatcg ctgggatttg ctactgggtg
     6781 ctcgagaacc gccagttgat gggcatcacg cttctcatcg gcggtgtcct tttcctggtg
     6841 gtcgcgctcg gaaccttggc ctctggtgtg cagccgcgcg atcgaacgcg tgctgggtcc
     6901 atcgattacg gcaatcgtga ctgaggaagc tcccttggat gagttcgagt ccgagcgatg
     6961 ggaagccgcc gaagagggga tcgagcttct gcactccggc gagaccgatc gcgcgatcga
     7021 cgagctcctt cgggtgacac gcgaagatcc gacgaacgaa tatgcctttc acttcctcgg
     7081 gcacgcttac tttcagaagg aggcgttccc ggagtctctc aagagttacg tcgaagcgct
     7141 caggctcgct cccgaatacg tcggcgcgat gatcggcgcg gggcagacgc ttcgcatgat
     7201 gggcgagtac gaccgcgcga ttcgcatggg tcggcgggtg cttcagaagg atgagaatga
     7261 cggcgacggc ctctttctcg tgggcgccgc acactttcaa aaaggagaaa accaggcggc
     7321 gaagcggttt ctcgagcggt tcttacagac caacccggag ctcgagattg cattggaagt
     7381 cgagggcatg ctgcaggtca ttcgcggcga ggtcttgcct ttcccaggcg ccgaggacac
     7441 ggtagagaac taacaggagg cagcggtgac ggcggacccg gcaaagaaac ggaaagtcga
     7501 gggctctccg agcagtcccg gagacgatct tcgagaccgg gccaggcgct ggcgcgaaga
     7561 ggacgtgaag ggcgcggctg cgcgccactc cttacgcaag cagcgcttca ccacactcag
     7621 cgacctcgag gttccggacc tgctcacgcc agccgacttc gatgaggatt atcaggagag
     7681 cctcggtttt cctggccagt ttccgtacac gcgtggggtg cagccgacga tgtaccgcgg
     7741 tcggctctgg acgatgcgga tgtttgcggg tttcgggtcc ccgcgacaga ccaacgagcg
     7801 ttttcgctac ctgctcgaac aggggcagac cgggctttct accgccttcg attttccgac
     7861 tttgatgggc tacgacagcg actctcctcg ctcgctcggc gaggtgggca tggtgggagt
     7921 tgccgtcgac accctccacg acatggagat cctcttcgac cagatcccgc tagacaaggt
     7981 cacgacgtcg atgacgatca acggccccgc cgccgttctc ctcggttttt acgtcgccct
     8041 ggcggacaaa cgcggcgtcc cgcgagaccg aatcggcggc acggttcaaa acgactgcct
     8101 caaagagttc atcgcgcagc acgcctgggt ggtgccgccc cgtccggcga tgcgcatcgt
     8161 caccgattcg atcgagttct gtgcaaagga agttccacgc tggaacagcg tcagcatcag
     8221 cggctatcac atccgtgagg caggctcgac ggcggcgcaa gagcttgcgt tcacgatcgc
     8281 ggatggactc gagtacgtga actggggcgt ggagcgcggc ttggatgtcg atgcgttcgc
     8341 gccgcgactc tcgttctttt tcgacgttca cagcgacttc ttcgaggaag tcgcgaagtt
     8401 ccgcgctgcc cgtcgcatgt gggcccggct gatgaaagag cggttcaatc ccaaggaccc
     8461 gcgctcgatg atgctgcgca cgcacgccca gacagccggc gtttcgctaa ccgcgcagca
     8521 gccgtacaac aacgtcgcgc gcgtcgcact ccaggccctc gctgcggtct tgggtggaac
     8581 tcagtccctt cacaccaaca gcctcgacga gaccttcgcg ctcccaactg aggaagccgt
     8641 gacggtcgcg cttcgcacgc agcagatcat cgccgaggag tcgggcgtga ccaacacgat
     8701 cgacccgctg gcgggcagtt acttcgtcga atggatgacc aatcgcatgg aagaagaggc
     8761 catgggttac attcgcaaga tcgacgagct cggcgggatg gtggaagcgg tcgagcgcgg
     8821 ctatccgcag cgcgagatcg ccgcttcggc gtacaggatg cagcagcaga tcgagagcgg
     8881 cgagaagacg ctggtgggcg tgaacaagta tgttcaggac gagcagcccg acattcctac
     8941 cctccgcgtc gacgagtcga tccagaagga gcaggtcgaa agcctggcca gggtcaaggc
     9001 ccagcgcgat gcgaaggccg tgtcgaaggc gctagcggcg atcgccgaag actgccgcgc
     9061 cgatcgcaac gtcatgcccg gcatcgtcga agccgccaag gtctactgca ccgaacaaga
     9121 gatctgcgac gtcttccgcg aagtattcgg ccagcaccag gaccgccccg agttctaggc
     9181 ctagttgccc gtcatgcgca gccacatggc gaccagcatg gcggcgacca tgagcgcgag
     9241 cccgaccgtg ccgatgaggt cctccacgcg cttgcgctga tggcggtcgt ccaaccagcc
     9301 gggggcagcg agatccggga gtgagctttt ttcagacggc agcggcgcag gaggcggtaa
     9361 gggcctttgc aggagcggat cggggagagc gggagcggga ttgatcacgt cgcgccattg
     9421 ttcttgtcgc gaccgtcgac tttgcacaac gtgccccgga tcgctcgggg tagcggatcg
     9481 ttcccgcagc actcgaacca cctttgtggt cgtgtccaag cgcttctttc ggaaatgact
     9541 cggccgtcgc cttggctttc cggggagctc cgggtcttcg tcggggtgcg tcacgggtga
     9601 tcctctttcc accgtccccc ggcggcttct tttcgagtat accgctcaat cgttcagtgt
     9661 catcgaacag ctcgactttt cgagagttcc catacaattc gaaagatgga tacacagcaa
     9721 gcggcggaag agatcgacgg cgacttctgg ggccgactgg tgttcacctc agagcatgcg
     9781 tcggctgaat tgcccgagcc gtggaaatgg ccggcagacg accgctggtt gatcgacacg
     9841 cattgggcgt ccgacctcgg cgccgctgca ttcacgcgaa gggtcgcgcg tctcatgaat
     9901 gcgcccgcgg tgctttcacg cttttcgcgc ttgctgatcg atccgaatcg cccgctcgat
     9961 tcggaaacgc tctttcgaga gaacgccgat ggcaaaaccg tgcatctgaa cgaaggactt
    10021 ctcgaagccg agcgccagcg acgaatcgat caattctatc atccgtacca tgcggcggtc
    10081 cgctcgatgg tcaaaaagag tcgcggtgac accgtattct ccattcacac gttcaccgac
    10141 aactacgaag gcgaggcgcg taccttggag gttggggtcc tcttcgacca tgacgaggag
    10201 ccggcgcagc ggctcgtacg ccatctccga ggggccggct tccacgctgc gccgaacgag
    10261 ccctggtcgg gcaagttggg tctcgcgtat tcaccagtcc ttcacgcgcg cgagtacggc
    10321 cgatgcgccc tcgagatcga ggcacggcag gacctgatcg tccaggaatc gtttgcgcaa
    10381 cgtcttgcgg aagcgctcgc gggcttcttc gcctaggctt cattcgatgc cacacatgcg
    10441 ctccgaacga accgggatgt cgtacctctc gtcgggtgac gagggcgctc cgctggttct
    10501 ctgtctgcac gggtttccgg atattccccg cacctgggcg cctctcaccg atcgcctcgt
    10561 cccggccggg taccgtgtga tccgtccttg gctgcctgga tacgcacctt cctcgctcga
    10621 agggccattc gatgtccctt cgctggctcg gaagatgctc tcgctcatcg acgagctttc
    10681 cccttcggaa ccaatccgca tcgtcgggca cgattggggc gccatcctca cccagttggt
    10741 gactgcgcaa tggccggagc gatttcgcgc ttcggcagcg cttgccgttc ctcacatgct
    10801 cgcgttcgag gcaaacgtgg agcgatatcc gcgacagctt ctaaagagcg cctacatggg
    10861 attcttccaa atcccggggc tgtccgatcg ggcgctgaag cttcggaact accgtctcat
    10921 cgagcgtctc tgggcgagct ggtcgccggg tttcgatccg ggagacgcgc acttcgagga
    10981 gctcaaggcc tgcttgaggt cgagcatgcc cgctcccctg cggtactacc gcgcgctgaa
    11041 atcacccaaa gcgatccgcg agattcgtgc gatcgtcgga gcggggccca tcgtggtgcc
    11101 gacactttat ctgcatggag agcgcgacgg ctgcatcggc cccgagctcg ccgatggcca
    11161 agagcagcac tacagcgccc tctttgaaac ggtgaagctc gctgaggcag ggcactgctt
    11221 gcacctcgag cgacccgcgg aagtgaacgc ggcgattctc cgctggttcg aagcgcacta
    11281 gccgcctctg gtgcctcgat gcggtcagac gccgacgagc ggaagctggc gccgaagatt
    11341 gatgtcgtcg gtgacgggga tcgggtagcg tccgctgaaa caggcactgc aaaaaccttc
    11401 gccgccgacc gcacgcttca atccttcgag cgagagatag ccgaggctgt ctgcggtcac
    11461 gtagtcgttc acctcggcca cggagtggtg cgcagcgatg agctcttcgc gtgtcggtgt
    11521 gtcgatgccg tagaagcagg gccagatcgt gggcggcgag ctgattcgca gatgaacttc
    11581 tttggccccg gagtcccgga tcatcttgac gatcttgcgg ctcgtggtgc cgcgcacgat
    11641 cgaatcgtcg atgacgacta cgcgctttcc ctcgagcagg ctttggacgg ggtgcagctt
    11701 gaggcgaacg ccgaagtggc ggatcgactg ggccggctcg atgaaggtgc ggccgacgta
    11761 gtgactccga atgagaccca gctcgaaagg aagccctgcg gcttcggcgt atccgagcgc
    11821 ggccgcgacg ccgctatcgg gaacggggac gaccagatcg gcctcgaccg gctggtcttc
    11881 ccagagggcg cgtcccatgc gtttgcgggc gtcgtagacg ctgattccgt cgaacgaggc
    11941 atcggggcgg gcaaagtaga cgtactcgaa gatgcacatg tatcgacgct cggcgtcggt
    12001 gcgaaacggc cgaaggctgc gaaggcccgt ctcgttgatc acgaccatct cccccgggtt
    12061 gatgtcgcga atgaacttgg caccgatcag ttgaaacgcc ggcggctccg acgcgatcac
    12121 gtacgcgccg tcgagctttc cgaggcagag agggcgaaat ccgtacgggt cccgcacggc
    12181 gacgagctcg tcaggcgaga gaaagaccag agagtacgcg ccctcgacct ggtgcaacgc
    12241 gtcggccacg cggtcgatca tcgtttcggc tttggagcgt gcgatgagat gaaccagcac
    12301 ctccgtgtcg ctgtccgacg agaagatcga accgtccttt tcgagttggt gccgaagtgc
    12361 aagaccgttg gtcaggttgc cgttgtgtgc gacggcgatc gagccatgac cgcaatcgac
    12421 cgcaagcggt tgtgcgttct tgatgtgact tccgccggcg gtcgtgtagc ggacatgtcc
    12481 gatggcgctg cttcccggca acttgtcgat ctggtcggcg gtgaagacat cttgcacgag
    12541 gcccatccct cggtgcacga agagctgcgc gccgttgctc gaaacgatgc ccgcggcctc
    12601 ttggcctcgg tgctgcaacg aatgcaggcc gagataagct agattcgagg cctcatcggc
    12661 tccaaacacg ccaaacacgc cgcattgatc atgaaaatga tcgtcgtccg ggtcccacac
    12721 cgggttttca tagcgccgca gcacccatgc gtccatgact cccgcatgcc cctggtccgt
    12781 gcctgcgcta gacctcgcgg gttgggatgc cgcggatact caagcgaatc gaatcggtcg
    12841 ttccccggag ggcttcgtcc tacggtggca aagcccgcgg cctggcagcc cttgctcgag
    12901 cggggtttcc ggtgcctgcg gcatatgcga tgcccggatg ggtaggcgac tcgttctttt
    12961 cgagcgtatt ggagatctcc gatcggccgc gagcgcttct gcgcacgccg cacgtgcccg
    13021 atgcacgact gcagacgatc gccgagcgcg tgcgcgcagc cacgcttccc caagacgtgg
    13081 ttcgcagtgt gcgcgatgcg gtcctcgcca tgcaggacga aggagcgacc agctttgccg
    13141 ttcgctcctc cgcgatgcac gaagacgagg aaggcgcgtc cgctgcgggc atgcatttga
    13201 ccatgctcaa cttgatccgc gaggacgaga tcctcgacgc gatcaaggct tgctgggcca
    13261 gcaccttccg gccccgagtg ctctcgtatt tgcgcgcgct cggggaggac gttccggtgt
    13321 cggtggggat cgtcattcag gccatggttc catcggagat ctcgggcgtt ctttttacgg
    13381 ccaatcccct caccggggat gccggcgagg tcgtgatcaa cgcagcgtac ggtctcggct
    13441 cttcggtcgt cgatggtcga gtgacgcccg acaccttccg cgtcgacaag gcgaccggtc
    13501 agctccgtga tcgaatcatc ggagacaagg cgcagcaaac tctgctcgag cgttcgggtg
    13561 gcgtgcgtga ggtcgccgtt gatccggccg accgtgaacg tgcgtcgctg agcgagcctc
    13621 agctcttgca cctcagcgag ctcgccgaca ggatcgagcg ccactttggc gatgcccgtg
    13681 atgtggagtg ggcgatcgcc gcacagcaag tgtatgtgct tcaagcgcgt cctatcgtcg
    13741 tctccgaagt gcgaagccgt cgcggggcta gccgggagag gccggtcgac cgtcgcaaga
    13801 tcgtctggag caacgcaaac atcggcgagg cgctgccagg ggtcgccacg cctttcacct
    13861 ggtccgtgct cagccagttc agtgacctcg gtttccgcag agcctttggc gcgatgggct
    13921 gtacggtgcc gcgtgatgcg gagctggtgg gcgatttccg ggggcgaatc tatctcaatc
    13981 tcaccgagtt cagtgccatc atggcgcaga tcccgtggat acatccctcg acgctcatgc
    14041 gactcggtgg tgggcagtat gcctcggagc tcgacgaggt cattgcggac cgtagctcga
    14101 ccgggttctt cctgcgtctt ccccagacgg tgtcgcgata cactagggag aactttcggt
    14161 tcaaggcccg cgtcgaagat ttcgaggctt acttctccga cgagcgcatg cggatgagcg
    14221 gaacggatcc gagacttctc gagccgagcg gtctcgaccg gatgctgggt gacgtggagc
    14281 atctactcga ctccaccggc tcgatcatgc tgaccgctta cgcgaacctg ctgacgacgg
    14341 tcttgttgtt gatcgggcta ctgcgagcgt ttgcgcaaga cctcggtcct ggtttctacc
    14401 gcgagctcct ttccggtctt caggacgtgg cgagcgcgcg gcccggtttc gtccttcgtc
    14461 agatcgctca gctcgccagg gaagaacccg agctggtggc gctcattcgt gacgccgatc
    14521 tttcggagct ccagatctcg aatctccccg cgggacgcac ccgggatgcg ctacgtcgct
    14581 tcctagacga ctacggctat cgcggcgtgc gggaagccga gattgcagcg ccgcgatggt
    14641 ccgaagaccc aacactcatg tttgccagca ttcgttcgca tcttgcgaac accgtcgacc
    14701 tcgacgtgcg tgagcgggac ctccaacaga ggcgcgccga cgcagaacgc aggctcgaga
    14761 agaacgtgcc tttcgtgatc agaccggcgg tgaacaagtt gctcgacctg gttcgccgct
    14821 atacgaggat gcgcgagcat ctgcgcgccc atgtggtcga ggtgctcgga atgtttcgtc
    14881 gcgtcgcgct cgaggcctcg cgtcgcatcg aagcgcgcga acccgaagcg ggccccgact
    14941 cggcgttctt cctcacgctg acggaggttc gccgtgtgct ctacgacgag aacgagagcg
    15001 tagcgattcg agtccagcgg agacggctag aatacgcacg aaaccaggcc ttgcccgagc
    15061 cgccgaccac gttcgtcgga tttccaccgg acgacctcgg cgttgcgacg gccacgaagc
    15121 gcctgcgcgg cctcgcggcg agccgtggga tcgtagaggg cagggtgcgc cttctcagca
    15181 agccgtccca ggccgccgat ttccggacgg gggagattct cgtcgtggcc gcagccgaca
    15241 ccggctgggc accgttgttc ttggcggcct cgggcgtgat caccgagctc gggggaccgc
    15301 tatcccacgc ggcgatcatc ctgcgtgagt acggcgttcc ggccgtggtc aacgtaacca
    15361 atgccactcg ctccctttgc gatggtgacc tcgttcgcct cgacggggac gccggaacgg
    15421 tggagatcct cagtggctcg agctgaagcc gtcgagcctg agatcatcca tcacgacgcc
    15481 gatctgctcg tgctgcacaa gcctccgggc cttccgacca gcgcaccggt ggcgagcgat
    15541 ccgtcgctcg ttcgctgggt ctccgaaaga ttcccggagc ttcgagcaca tgccacgtct
    15601 cggctggact ctcaggtgag cggtgtcgtg actttcgcgc tgaaccgcga agcgaaccag
    15661 catttgctcg atgcacgcaa atctgggacc tacgagcgga tctatctcgg catcacggtt
    15721 cacgagctcg ccgacgatgc gggacgatgg gattggccga tctccatcga ccctcgtgac
    15781 cccaagcatc gccgtgccgg ggcgggtcgt ggcgagcgtc acgccatgac ccggtacgaa
    15841 gtggcagcgc ggagatcggg agcgactctg cttcgtttga tgccagcgac ggggcggaca
    15901 caccaactga gggtccatgc ggccgaggcc ggggcgccgc tctttggaga tcccgcgtat
    15961 ggaggcgagc gacgggccgt gttgccagat ggcgccgtcg tcaccgcgcg ccgagtgatg
    16021 cttcactgtg ctcgcgtgag ctttccgtcg ctgtccggcc ctgaacgcat cgtgatcgag
    16081 gcggcagtgc gacccgacat gcagcgtgtt tggcaggctt tcggtggggc cgcgtcggag
    16141 cttcagccct gacgcttcgt cgtgaatttc gttcctgggg gaacgtctac cggtctgttg
    16201 aaatgagctt cgagctcgcg atgcaggatt tcctgcacgt cttcttgcag tccgagcggg
    16261 tggcgaatca atcgctcgat gtttcgctgg tatacgaaga ggcgtcgctg ttcttcgcct
    16321 ccggcgttgg tcgccatttc gtcgagtagc agaggtgtgc gtgggtccac gccttcggcc
    16381 acgacttcga ggccgggcag gtcggtgacc atgatgaggt tgccttcgag ccgttgtgca
    16441 agctcctcgg gcagctcccg cagggcggac tgcacgattt tttcgaaacg tgcgtgcgac
    16501 ggcgaccatg gagctcttgg cagtaggccg tcgagatggc gggtcttcaa gaacgccagc
    16561 gaggcctcat cggggcgctc ctgacgatcg cgcagaagtc cgagatagta caagatatcg
    16621 ggcgatgcgt ccgagggctc ggcagcccgt tcgatgagag ctgccgcctc ttcgatttgg
    16681 tcgagctcga agtgcgcccg cccgatcaag aagtcgagct gctgattttc gaagggcccc
    16741 tcgggaagcc ccagcaagag ctccttggcc gcggcgtggt cgccctgttg gagcaacgcg
    16801 tcgaccttga gaaccgaagc atccgcaatg tcgtccgccg tctggcagct ttcgagcgcc
    16861 tggtcgagaa cgcgtatcgc ttcggcgaag cttccaagtg gatgaatcaa gagctcagcg
    16921 gcgttgagca tggcctcgag gtagtgctcg tcgaggtcga tggcttgccg gtagtgttcg
    16981 agcgccaagt cgaggttgcc gttggctgca tggatgtagc cgatgaggtt gtgcgcgtcg
    17041 ggcgagtcgc cgtcgagctc caaggtctgc tcggccgacg ccattgcgcc gttcaaatca
    17101 ccttgcgtaa ccagctccca gccgcggtcg aggtgcgcgc tcaagcggtc catgaatcct
    17161 tcgttctgtt cggacatcgt caattcgcaa agttacgtgg ccggtatgcg acgtcaagcg
    17221 gaaccgtgag ctatcatgct ttggaatggt taggtttttc accgctttag gcatcgtggc
    17281 gatctgttcc tgtgtcaacg agccattgcc ggtgggccag gcgatctccg ttggcaccgc
    17341 tgagcatgga tacctccacg atgcggtccg cttggaggat cgaggcgatg ggtatcgacg
    17401 catacgcccg ggtgaaggca gccggtacgg aaccccgacc ctggtcggcg cgatcgagcg
    17461 tgctgcgcag gaagtcgccc gagcctttcc tggaggaaac ccgcttcgtg tcggcgacct
    17521 atcgaatccc catggaggac aacattcgcg ccaccgtacc catcgcgcgg ggcgtgatgc
    17581 cgaccttctg ttctttgcag aggacggcgg cggggtggcg gcgccgagcg acggatggcc
    17641 ggagttcgac cggttcggcc tctcggcgct cggcggtcgc gtctttggtt tcgatgaagc
    17701 gcgcaactgg cacctcgttc gaacgctggt catggacgag cgcgcccggg tgaaatggct
    17761 tttctgttcc aacgagctca aggcgaggct tctgcgctat gcagcacggc atgaacactc
    17821 gccgcgggcg atccttcgag cgacctggat tcttcatcaa ccgtcggcgg gcgattccca
    17881 tcgcgaccac ttccatcttc gtgttggttg cggggaattt gagcggcact tcggctgcga
    17941 ggaacaagcc ccgcattggc cctggctgag cgatcccgcg cgcaaggaag acatacgggc
    18001 gaacccggct gcgagcgacg agcacttggt tcgctggtta ttcgccgagg agcatgtgga
    18061 tgagagacgc gacgtcatct ggcgtcgcct cgacgccgca gcgcagccat ccacggtcgc
    18121 tcgtcgctag gtcggtgaga tccctgtgcc gttcggtggc accccagatc aggacggccg
    18181 agctcttcgg cagctcatgg gcgagagtcg caatcgtcgt tggttgcacg gatgcttcga
    18241 cacaatcgat caccacgagc gggctcaagg acgcggtcgc cttcagattg tccagaaacg
    18301 ccaccacgtc ttcgatctgc tgaaccgcgg cggagcttcc aacctgcttg gcgattctgg
    18361 cgcagcgctc gcgactgcgc gtcgccacga agaccatggg cagggccgag ccctgcggct
    18421 gcatcgtcgg gtacttcgac gtgtcggtct ccggtgcggg accgggtgcg cgccgtgtca
    18481 ctcccgagtc ttgacgatcg cggccgagct tgcgcgttgc atctgcgcgc actgtttggc
    18541 gtggtggttg actgttcacc atgtcagcca tcgtcagcat gggcgcgaga ttctgcatca
    18601 ccgcttcgct cgcctcgtcg cctacgtaga acgccatggc cgttcgtagg tgcttgtcga
    18661 tgaagcgatg gagcggctca ccggtggtgg ggatctcgtg ctctcgggcc atgtgcaggg
    18721 cgcggtgaag gattcggagc cgaacctccg gggtcgcgac cgtttcaatc gcctctcgca
    18781 ccatttgggc catcggggtt tccctctgca tgtactcctc atccccagaa ccagcgtcag
    18841 ttcgagatac cactacaccg taatattacc gtgctttggc ctctcagcca accctccacc
    18901 cctggtatcg cattcggcgg ccgtggtaca tggctgacat gctggtcatg ggggtcgatg
    18961 agaacgggct gggacccagg ctagggccca tggtggccac cgccctcacg ctggaaacgc
    19021 cccgatacgc gagggccttc ctcacgaagc gaggcctggc cctcggtctg accgacagca
    19081 aacagaccgg cggattcggc cgcctggggt tcaccgaatc ggctgccctg ggcgtattga
    19141 ggcttgccgg cctggggacc ccaggatcgg cggacgagct cctggaaaga gtgtcgccag
    19201 ggacctcgga gcacattcgg tgccgctgtc ccgatgactc gagcgctgcg caatgctggg
    19261 ccgtcgattt tccgctgccc ggtttcgggg gcgacgcatc cttcggcgta gacttgctcg
    19321 aacgcttggt cggtcgatcc aggcttcgca tcgtggatgt gcagagccgc atcatctgtg
    19381 ccgggctgct caacgaaagg ctcgcagcag gtcagaacaa gctcgccgtg gacctcgagc
    19441 tcttcgagca gctgatcgcc tcggtgcaaa gccgccatgg ctcgccgcta ctggccgttt
    19501 gcggaatgat cggcggcatt cgtgattacc agtcccgctt ctcccggttt gaagccggcc
    19561 gggtcaagga gctcaggcgc cgccgagggc agcgaaggta ttcgatcgac cgcttgggcg
    19621 aagttcggtt cgaggtcgat gccgacgcgc gccaccttcc ggtcgcgctc gcttcgatcg
    19681 ttgggaagta ccttcgcgag atttgcatgc gccggatcgg tgagttctat cgacgggacg
    19741 acccggcctt ggagctctcg agcggctacc acgatccggt gacaacgcgt ttcatcgacg
    19801 ccaccgagcc cgcgcgccgg cgtctgcaga tcgcacctga ctgcttcagg cggcaagcat
    19861 gaaccctcag ctgagagtca gccatgtcgg agcgtgacag gattcctcgc tgggcgcttt
    19921 ggctcggagc tttgctgctc ttcggatggc tgctctacac gctgcgcggc gttttgggac
    19981 cggtgttctt cgcgtttctg atcgcttaca tgctcgatcc tctggtcgac cgaatcgagc
    20041 aaagccgtct gctccgcggc agagccatcg gtcgcggcgt gggcattgcg gtgctcctcg
    20101 ccggcctctt cgcggcctcg gcgtttgcca tcttggtcgt cgctccgatg gtctacgagg
    20161 aggtctcgag cttcgtgcta cgcctccctg ggctcgtcgc gcggagccgc gccgagtggg
    20221 agccagccct cgcggaactt ggattgcgtg ttcccgcctc cgtgggcgag gcgttcgaag
    20281 agcttcacct cgacatgcag tcggtgctca ccaagggcta cacccctgcg accgcgtttg
    20341 cgaaatccct cctcggcgga acggcttccg cactcgcggc gatcatcgcc tcgctgatgg
    20401 tgccggtgtt cgcgttctat ctcctctatg atttcgatgg gctcgtcgcc aaagctggag
    20461 aactgattcc tccgcgcctt cgaccgcaga cgaacagctt ctttgccgat gtagatgccg
    20521 ttctcggaca gttcttccgc ggtcagttca ccgtcatggc cattctctcc gctctctatt
    20581 cgctgggcta cggcgtcatt ggagtgccgc tggccctgcc gatcggactc atggccgggt
    20641 tcctttcgtt cattccctac gtggggagct cgcttgcgct agcgactgcc ttgctgatga
    20701 ccgcgctcga ttggcagggt tggacgcaaa tattctgggt gctcggggtg cacgcgatca
    20761 ttcaaggctt ggagggcttc gtcatcaccc cgaagatcat gggtgacacc gtcgggatct
    20821 ccgcgatcgc agtcctgctt tccctcctgg tcggcgccga gctcctgggt ttcacggggg
    20881 tgctgctcgc ggtgcctgcc gcggccgtca ccaagattct cgtcacgcga atcgaccagc
    20941 gttaccgtca atccgagttc ttcgcaggac agaagcccga tgcgaacgaa gtcaccgatc
    21001 actcgaaaga tgagtcacgt cgagcttgac catgattgtc gcggcgcggc gctgaacgcg
    21061 tgccgattgc agcaatgcca gccagggcgg ctcgaacgta tcggggggcg ttgcgatcca
    21121 ggcctcgatg agctcggcgt tcttctgggc ctgcacctcg cccgagacct cgatgtagag
    21181 ctcgagcgtg tcatcgctgc ctgacttcac cgagaacgcg atcgcctgcg catcgtcgag
    21241 cggggatgcg tcactgccga atctcgccgc tcctgccagc gccgacttcc agtgcgctgg
    21301 tggttcgacg atcgccgcga tcgccgctcc gatgctcagc ctcggccaga gtcgcgctat
    21361 cgtggctcgc tcgagaaggc tcgacgccgt ccctcgtcgc acttccatga tttcggtcac
    21421 ggttctgacg gacccggtca cgatcgtgcg ctcatcgagt agggcgatgg cgctctgacc
    21481 gtcgcgactc gagatcaatg gaccagcggg actgtcgaga cggagcaccg acccgccccg
    21541 tctctcgacg agcaagcgat ggcatgccgc gagcgcctct ccatcggcgc tgcttccttg
    21601 caacatcaat ccgatcgctt gaaacggttc ctcgtcgggt ccgcggaccc agacggtagc
    21661 gtcgctcaac gaggccagcg gatcgaagcc gcaggccgtc tcgatctcgc tgagcgcgtc
    21721 gtcaggcatg aaggctttca agaggattct agccgcctcg gtgcgttcca aggcgcgcat
    21781 gtcgatccgc aagagcgctc gcgccgtgtt cggaaatggt tccagcgatc ggcgggcgcg
    21841 cagggaccgt ccatgtcgat tcgccgcgaa tagcagggcg gccatcatga tcgagacgag
    21901 agccagcact ttgagcgagc gagacatcgg tcgagggtag gaagttcttt tccccgggca
    21961 agccatcgtg ctaacgcctg cccatgccgt ggcgccgaat gcgattacgc aatgccgaag
    22021 ttctcgcccg ctgcgacgag agcggggact tggttgccaa tggcggccgg gtcgaagtcc
    22081 gatacaaacc caacgacgga cgcgcctact ttgccgcggc ttcgaacctg acgcccccgg
    22141 ctggcgcgct tcaaatcgag ccggactcgt attgcggccc gggcgaatcc gtccagaagc
    22201 agtcgaaacc ctcgaagaaa tcggccgcct ccgcctccgc gcccgaatca ccccagggtg
    22261 acgagctgct cgtctacgcg gatggagcat gcagcgggaa tccgggtcct gccggagttg
    22321 gcgtcgtcgc gctctggggg gatcagcggc gtgagctgag tgagtacatc ggcgaggcga
    22381 cgaacaacat cgccgagctc accggcatac tccgcgccgc cgagcttgcc caagagctgg
    22441 gcaggccgct gcggctctac accgattcga agtattcgat tggcgtcttg actcagggtt
    22501 ggaaggccaa ggcgaacaaa gagctcgttg ctgccgtgcg cgctgcattg caggcgcagc
    22561 gagacgccaa gctttttcac gtgcgcggcc atcaaggggt ccgcctcaac gagcacgccg
    22621 acgagcttgc cgttctcgcg gtccagaaca ggaaatctac gggctgggtc gatcgctaaa
    22681 tcgccgcgcg gcttctgcga gcgtgaccag ctcgtagccc gcgtcgcgaa tcacgcggac
    22741 tgcagcccga atcacaccga gcttctcggt ggcgcctcgt ttcagatccg cgcggtgaag
    22801 cgccagtgtt tcgagaccat cctcaattgc atcggacgcg tcgaggccat ggagctcgag
    22861 gttgatcagc tcctttccga gcatttgctt gctgagccgt tcggccattc gctttccgcc
    22921 gagcacgagg gacgtgccga tgaaaggcag ctgcagtggc gtgacgccga tcggaagctc
    22981 gagaattcca tcgccccttc gccagtacgg gcggccaacg cggtatggtt gtcgcggtgc
    23041 tcgcatgacc gccggcgtat cgagaacgct cgcgctactg cgcccgatcg ctctgaacgc
    23101 gctgagggcc agagcctttg cggcgtagta gctcgggcac ggaaagacgc tcgaatcgta
    23161 ttcgacgcct gccacatcga gcgcgctgaa gagcgcatcg ctgaccgtgt atccgggggc
    23221 gcgaaaaccc gagggccgag tgccgcaggc ccgctcgatg atcgctgctc cgcgctcgat
    23281 ttcctcgcgg acctccactg ctgcgcgccg cgttagatca tagtggtggt gatacgagtg
    23341 gtttccgatc tcgtgtcctg cgcgatggag tcgggcgacg ctggccctgt tctcgtcgcg
    23401 ttcgagatcc tccccgatca cgaagaacgt ggctgggatc gactcctcct cgagccaggc
    23461 ttccagccta ggtactgcag cgtcgtagac cgcatgggct gccgggcctc ttagctgctc
    23521 gaggccatgg atctccgcgt aacgaggcgt ttcgtccaag tcgatcgata tcgatgcgag
    23581 tttgagggtc accctttgat ccggattgcg atgaagagct tggccaggtt cttcaagacg
    23641 ttgggaacgc gcttcaccaa accgacggtc ggaggtctct tctcaagaac acggatgggg
    23701 atctcgcgga cgcaaaggcc agcccgctcc gcccggatca ccagctcgga ggcgaacaga
    23761 tcgcggtcga ccaggcatgc tcgagcgatc ggatcgaccg gtttccgtcg gaacgccttc
    23821 agcccgtggg tgtccgtgcc cttgaatccg agcgcgagcc gcagcattcc gttgatcacg
    23881 atcgtgccga gtcttcggaa gcgaggtcgt tggtccatcg ctccttccat gaccttcgat
    23941 ccgaccacga agtcgacggc ccgatcctcc agcagcgcca tcgcgcgctg gtagaaatcc
    24001 gtgtcgcata gatcgatctc gtcacaaatc acgatctcgc ccttcgcatg gaggattcca
    24061 tcacgaagag cgctgccgta gtttggctcg ccggtgtggc gacagacgag ctgctcgaaa
    24121 cgctcggcga gagcgagcgc ctcctcgaag gtgcggtccg tcgaaccatt ctccgcgacg
    24181 atgagctcat agggccacgg aagagcgctc agccggtcga gcaggtcgat cagggcggcg
    24241 cgcaaaatcg cctcctcgtt gtacacgggg atgacgatcg agatccgggg cggcgcgttc
    24301 gactcagtca acgggcggca ctctctaacg cgtgcattcc acggtcaagc caatgctgcg
    24361 agcaccccat gcgccgcctc caagtcgatc tgcaggtctc ctaggcatgc tgggagcgcg
    24421 gcggtttgac cgctggataa aatggtttgc gtcgcgccgt catcgaggcg agctcgtccg
    24481 gcgagaaccg tgagcgagcg gagccggttc tctgcgatga gaacgagatg tccggtgccg
    24541 gtgagacgtc gcaacgagaa ctgttccgag cgtgcggggc cagtcttgct ggcgagaatc
    24601 tcgagacgcg ccgtatcccg ggttcgtgcc ggtccggaac ggtggcgcac ctctgacaga
    24661 agcgctgcgc ctcggggacg gtcccatcgc gtcacatcga gcgcgcgttc tcggtgaagc
    24721 gcgcgcggtt ttccttcggg gtcgaggcgg ccggcactgt cgtactttcg attccaatcc
    24781 cagtagcggt aggtgagacc acgacgacct ggagcaacgc gctgaggctc caggagcagc
    24841 acgcccttac cgatcgcgtg gggcgttcca ggttcgatca gaaaaacgtc acccggcgac
    24901 accggcacga atgacatcag cgcgtcgacc gctccttcct catcgatcgc ggtttcgacc
    24961 tcctgcctgg acactccgtc acgaaagccc aggtagagac ccgcgcccgc gtccgcttcg
    25021 atgatgtacc aggcctcggg cttcccgctc tcgtcggccg caagcttcgg gtcgtcgtcg
    25081 cgcggatgga tctgcacgga gagatcgtcc gcagcgtcga ccagcttgac caggagcgcg
    25141 gtcgagccga gctccgcttc gaccccgagc aaggcggggt ccgcgcgaag gacttcatcg
    25201 agcgtcggtc catcgaccag tctgctcggg aagtctggct ccaccgaaag ctcccaggct
    25261 tcgcccacag ggccacgatc ctgcaagccc gcgttcgcct tcagccgtcc ggtgatgcgg
    25321 cggccccccc agggggtccg cgacgggggc gtgaaattgt ccggctgaag gaggagaggg
    25381 cgccgccaat tttttggtcc tactttatcc cacatgtaag gtgacaccga atcctatggt
    25441 agcgccccgg gaggtttctg atgggttatt tgggcaaaga acgacgtatt catcgcattt
    25501 tcgtgactcg caacagtgaa taccacgtcc gccggaacgt gtgcgtcggc gttcgagatc
    25561 gccggagcgg cgagtggctc ggcggccacc tcgcggtgcg cagcacggtt tcgggcggcc
    25621 tgaagttcca cgacaacgga gcgatcagcg cttcggaggg cctccccacg gtcggcgagt
    25681 cgctgttctt catcgcggca ggccgagatc tgatcaccag cccggtgctc aacgtcgagc
    25741 gccccgcccg agagatcgtc cacaactacc caatctaagc actggccaga cgggatgcta
    25801 tatcctgccc cgtgtcgtcg gagatgaaga ccattcgagt ggttgccgcc ctcgtcgagc
    25861 gtgacgggcg gtatctgatc acgcagcgtc gcgagagcgc ggttcttccc tcgctttggg
    25921 acttccccgg cggtcgagtc gaagacggtg aaaccgatga agccgctttg gcacgcgaag
    25981 tgagcgaacg actcggcgca acggtggagg tcgggcaact gatatcgttc gtcaaccatc
    26041 cgtacgagaa gtacgcggtc gacttgtatc tctatcagtg tgagttgctc agcgatcggc
    26101 ttcactgccg cgccgtcaaa gactacgcat gggtgacctc caaggaaatg gagtcttacg
    26161 cttttacccc cgtggatgaa gcctcgatga gcaagcttct cggcgagggc tgagcgccat
    26221 ccctagacgt cgtagaaaat cgggggtcgc tggcggatga aacgcaagac caagttgcgg
    26281 gcctcgtcgg acagattttc gaactgtatg cccatcccgg gctcggactc ggacatgaaa
    26341 tccatcgggt cgcgaacgaa gcgaacgtaa ccctcgcact cgaactcgaa gccaccgggc
    26401 agggtcacca gcattctcac cgaggtgtct ttcggaaggg cctcgtaagt ggcgagaaac
    26461 accccgccgt gggcgatttc accgctgaac cctacataga agttcgactg agtagtggcg
    26521 ccgaggttgg cttcgaccgc ttgccgctcg cccttgggaa tcggcggagg aggcggcagg
    26581 agcgatgtgg gaatcggctg aggtgcaccg gaacgcggcc tgccggattg gggccgctcg
    26641 gaaggtcgat ggctgagtgg cggtcgactc gaaggcggtc gactcgaagg cggtcggctc
    26701 gagggcggtc ggatcgacgg tgggacgctc gccggaggca ggatcgaggg agctacgcgg
    26761 accgggggcg cgcttgcagg cggtcgactc gaagggggaa cggactgcgg tggcgcggac
    26821 tgcggtgacc ccggcaaggg gcgcgtggat tggggtggcg tggattgcgg cggtgccgat
    26881 cgaggccggg tggactgcgg cggcctggat tgcggtgctg tgctcgccag gggagtcagc
    26941 tccggtcgaa cgctcggctt gttcgtgagc gggaataaga tactgatgac gtcggcgatg
    27001 ctctcggttg cggtttcaac gccggcgtgc tcgctcttca cgtcttgaag aagtgccagt
    27061 gtttggctga gcgtgccgag cgcgttcctc acgcaggtct ttccgtcgac ctcggtcgat
    27121 gcgtactcgg cttcgaagag ggcgccgatg gacttcgcga cattctgcgt cagtccaagt
    27181 acctcctcgg gaacctccga gtctttctgt agcgcttcga gtgctttgcc gagcaattca
    27241 cgagcctgtc gtgcctcggc cgctgatgaa taagccatgc ctgatttccc cgcctgcttg
    27301 cttcgtttca gaataccggg actcgccgtc gggtcaaagc tccttgacga ataccacgcg
    27361 tgagcggcct ggacctgcgt agtcggcgac ttcgttactc tcgaaaccca tcgattgatg
    27421 aaagcgaacg gggccctcgt gccccgctga tgcaatggat ttgatgcgct tcatgccggc
    27481 agcccggcag cgctctgaga aatgctcgta aagatgcttc ccgacgctgc gacgtcggtg
    27541 gtctggatga attccgacca ggtgtacgta gccggtggcc gatggggcag gcaccatgac
    27601 gccgaaaagg aacccgatca gctgtccatc gcgctcggcc atgagagcgt gatcgccgaa
    27661 ttcgtagaag aacatcggat cggcgcgctt tccagcgggt ccgccccacc actggtcgag
    27721 gaccgagatg atatagtcgt aatcgacctt cgtgattccc cggatctcca tgtccccaaa
    27781 cagcgaataa cacgaacacc acattaaagc acggcgaccc caatcgaagc atgcctccgt
    27841 ccccagcctc atctgaacgc gtcgaaattc tgttctgtcc tttctgtggt gacggcttcg
    27901 aggaccggag cgattgcccg gatcacgatc tcgcgctgct tccgctcgac gagcttccgc
    27961 ggagcggcca gcgtcagctc cagggcgtca cgttcttcgc ggacccgcga ctcgggcgag
    28021 gtcccgtgct gctggggatc tctttggcgc tactgggctt tctcgtgccg atggttcgct
    28081 acggagacgt cgtcgagtcg gcgctggagg tcgcgatcga gggcgcgggc aatctatggc
    28141 tcgcgcccgg cgcgggcctg gctgtgctca tgattctatg gcttcggcgg agcaggtcgg
    28201 cgatgagcgg tgcgcgcgcg gcggtgtttg gcctggccct cggcggcatt ttgccattgc
    28261 tctacaccgg tcgccgaatc gggatgctgg cggagtcgtt cagcacgagc ctcgagtggc
    28321 aatggggcct ctggttgatg atcgctgggt tcgtagcggc cgccttgggg agcgtccggc
    28381 tcggaggccc taggctgcgc ccggaatcgt aggccggaag atatatccct gctgctgcac
    28441 cacgcagacc cgattctggc ctgcgacccg ggctcgagcg acggcgatac tggctgcgtc
    28501 caggagctcg ttgtgggatc gaatgctccc gctcggatac aagccaaccc cgatagcaac
    28561 cgtgacctcg aaagtggcgc cgttcagctc gaacggccga aacgccacct ctgaaacgat
    28621 gcgatcggcc agggtgagcg cccgagcagg acgggtgttg ggtagcaaaa gaccgaactc
    28681 ggcggtgcga aatcgtgccg cgatgtcggt cgcgcggatc gttcgttgca agcgctccga
    28741 aacctcatcg agcattcgtg ccgcaagatc agccccgcgc tcggtcacga gcgctcgaaa
    28801 gtcgtcgacc gccacgatgc agcaagcgag tggatccaaa tgacgctgcg cttcgtcgaa
    28861 ctcgctttcc aggcgttcgt ggaaatagcg gtgatcgggc atcgaacgga gttggtcgtg
    28921 cagcgacgcg tagttgagct ccctctttgc gaactgaacg tcgtcgcgcg tccgtttgac
    28981 gcgaagcatg ttttcaatcc gagcgaggag ctcttcgtca tcgagcggct tggcgatgtg
    29041 atcgtccgcg ccggttcgca gaccctggac tcgcgcactg agatctccag gagccgtgag
    29101 aaggaccacg gggacgaagc ggtccttggt gatgcttttg atgactctgc aagcgtcaac
    29161 gccgctgaga cccagcatgt cgacgtcgag gaggacgaga tcgaccggct gggcacggac
    29221 ggcgtcgatc gccttctgac cgctgctggt cagaacgacg gcgaatcctc tcgtctggag
    29281 gacctgggag atgtggtcgc gcgttgcgcg atcgtcatcg gcaacgacta cgaccgcctt
    29341 tgaactaggc ccagcagtcc ccacccggac agagtacccg aggcgaaagg gtttcgcgac
    29401 ccttcgtcgc cgagtttccc ttggcgcccg ggccgtctat actccagatc gacgcaccct
    29461 tctgggggcg cagggcctgg atggagctca agcagcaact caaattatcg caacagctca
    29521 tcatgacgcc gcaacttcag caggcgatca agctgttgca aatgtcgcgc atggagctga
    29581 ccgagctcgt gcaggaggag cttctcgaga acccaatcct cgaagagggg atggaggcgc
    29641 gcgactcgac cagtgcgggc actgacgagg cgccatcggc ggaggtcaag gctcacgaac
    29701 aaaagaagga cgagatcgac tgggagcgct acctcgagaa ccagtcgaac gaggcgccca
    29761 tgccctcgat tcgacgcggg acggacgagg agatgcccgg catcgaagcc acggtttcga
    29821 gcgccgagaa tctcgatgac catctcgggt ggcaggttcg catgggcgac ttcgtcgagg
    29881 acgaacgccg ctttgcctcc ctggtgatcg gaaacctcga cgacaacggg tatctcaagc
    29941 tcgagggact ggctcccgag gaggtggtac cgcgtctcgc ggccgaagcc gatctcgatc
    30001 cggaggacgc cgaagaagtg ctggcgatga tccagcagtt cgatccaatc ggtgtcgcct
    30061 cgaggtcact cgaggagtgt ttgctgattc aagccaagca ctacggcatg gacgagctcg
    30121 tgcttcggat catcggagac catctcggtg atctcgagaa acggcactac gccgccatcg
    30181 ccaaggtctg cggctgtgcc ctcgacgaag tatacgacgt cgcgcagatc atcgccgagc
    30241 tcgaaccgcg tcccgctcga cgtttccaga ccgaggaacc ccggtacatc atcccggacg
    30301 tctacgttca ccgcgtgggg gacgaatact acgtcgtggc gaatgacgac gggatgccca
    30361 agctaaaaat cagcggcttc taccgctcgg cgatggcgga caacccgaag gccaaggaat
    30421 acatccagga caagcttcgc agtgcgcagt ggctgattcg cagcatcgat cagcgccgca
    30481 agaccatcgt caaagtcacc gagtgcatcg tcgaaaaaca gcacgatttt ttcgacaagg
    30541 gcatcgagca cctgaagccc atgattctcc gggacgtggc cgaagcggtc ggcatgcacg
    30601 aaagcacgat ttcccgcgtg accagcaaca agtacgtcca cacgcaccgt ggcacttttg
    30661 agttgaagta cttcttcaac agcgccataa ggcgtgatca tcagaacgac atcgcctcgg
    30721 aaagcgtcaa acaagcgatc aaaggcatca ttgggggcga ggacccgaaa gccccgttgt
    30781 cggatcagcg aatcgttgaa atacttgcgc aagacgatat caagattgcc cgccgaacgg
    30841 tcgcgaagta tcgcgagatg ctcgggatcc tcagctcatc aaaacggaag aagtactttt
    30901 agcctctgga ggttcgatgc gtattagcta cacatttcgc aacatggagt cctcggacgc
    30961 catcaagagc cacgcatccg agaagatcgg caagatccag aagtacatgc gggcgcctct
    31021 gcatgccgag ttcacctttt cgatggaacg gcacctgcac cgggtcgact tgaacctgac
    31081 cggtgacggg caccagtact ctggtcacgg cgagtccgag gacatgtaca cgaccatcga
    31141 ccaggtcgtt gacaagatcg accggcaggt acgtgacacc aaggcatcgg agagcgaccg
    31201 tcgcaggcac ggcggcgaaa ccctccggcg ctctgaagac tgatccacat tgcgcgggag
    31261 cagcgggtgg ggtatcgcct ggccttcatg ctcgccgacc ttctacatcc cgagcgcgtg
    31321 cgtaccgggc tcatccttcc ggacaaagag gctgcgttgc gagccgtggc ctccttgctg
    31381 acgatgccgg aaggagaggt caacgaagag gtcgtctatg acgttctggt cgaccgcgag
    31441 cgtttggcga gcaccggcat cggtagcgca gtggcgattc ctcacggtcg ctacgatggg
    31501 atggaggagc tgcgggccgc ggtggcgatc tgcccaggcg gtgtcgactt cgacgccgtg
    31561 gaccagcttc cggtgaagat tctcgttggc atcatagggc cgaagtcgat gccgcagaag
    31621 cacctggccg ccttggctgg agtatctcgt cttcttcgaa tggacgccaa gcgcgagcag
    31681 ctcttgagag cggtcgaccg caccgaagcg tatcgcgcat tgacggagtc gtgatgggca
    31741 gacagtcgcc gatcgctggc gtatgatggg gcagtgactg agtcctcctc ttctcggcaa
    31801 ggcggccttg ccagcatgcg ggtcgatcgt ggaatcaccg ttgcgcgtct tctggccgag
    31861 ccccgcctca agcccatcct gagattggtt gccggtcagc ggggagaaga gcgcgccatc
    31921 gaccatccgc gcgtgcagaa gcccggactc gctctcgctg ggcataccca cggtgtggtg
    31981 ccgacccgag tgcagattct cggtgaaacc gagatcacgt acctcgacac cctctcgccc
    32041 gacgagcaac tcaagcgcgt gactcagctc ttcgacctag ggctctcgtt ggtcgtcgtc
    32101 acccgaggag tggaacctcc ggcagcgctc atccaggctg cgcaggaaac aggaacgcct
    32161 ctggcagtgg cagcaccccg gtccagccgt gcgattcagg cgatccatct cgtgctcgat
    32221 ggaattctcg cgccttccca aacccggcac ggggtgctga tggacgtgca tggaatcggg
    32281 accttgcttc tcgggccgag cggcatcggg
//
LOCUS       DASRZF010000013         7847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2757313, whole genome shotgun sequence.
ACCESSION   DASRZF010000013 DASRZF010000000
VERSION     DASRZF010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 7847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7847
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2757313"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VFG22_00650"
     CDS             <1..296
                     /locus_tag="VFG22_00650"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6414771.1"
                     /translation="RELEHLLINAGVFASGGTIEAEDLAIESGRVTQSPSAGASNYVA
                     FKETERDRIIATLNAHGWNRAKAARSLGMARRTFYRRLKEHAIELPTSTGTRD"
     gene            complement(293..1222)
                     /locus_tag="VFG22_00655"
     CDS             complement(293..1222)
                     /locus_tag="VFG22_00655"
                     /inference="COORDINATES: protein motif:HMM:NF017736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET6414772.1"
                     /translation="MSSTGVWRGVAVVFLVLFLSAQAHAQRKHTVQTGQTLGAIAQRY
                     RVSVTNLAAANSLKKTSNLCVGQVLRIPPKGVIYVYPGQTLTGIAQLNKVSKEALARA
                     NGLKPESTLRVGQRLELPGYSANAKASKAQNTVLHGLVTLERPASNETLRVHLFDERG
                     KLDAKARARLGRFLRDRETDDVKLPNVRLMRALTYLADHFNGRTIVVVSAYRADKNSD
                     AGSSRHGTGEALDIRVEGVPNEVLRDYCLTLSKVGVGYYPRSSFVHIDVRSKAVYWVD
                     WSRPGEAPLYLPPGETPEKYDAGVSSSVQPASL"
     gene            1547..1870
                     /locus_tag="VFG22_00660"
     CDS             1547..1870
                     /locus_tag="VFG22_00660"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET6414773.1"
                     /translation="MTYDPEANEAMQKPDTMGASASEANDQQRASKPVANKKSGNRPN
                     NVRKIRIERMMSKAELARRANLSVLTIDRVEKGFGCRMDTKRKILEALGLRLADRVRV
                     FGEEE"
     gene            1880..2929
                     /gene="pilM"
                     /locus_tag="VFG22_00665"
     CDS             1880..2929
                     /gene="pilM"
                     /locus_tag="VFG22_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HET6414774.1"
                     /translation="MPSEGKNLIGVDIGSSSIKVCEIKEGRRGARTITRFGYHPLPAE
                     TIVDGHIINSGAVVEGLEKLFARSKRRNVALRASGHSVIIKKIAMPLMTAAELQEQIN
                     WEAEQHIPFDLDEVHVDYEVLHERTEHGQMDVLLVAAKKEEITDLTNLAMEARLRPIV
                     VDLDAFTVQNVFEAGYGAAASDETTVLVHCGASTTTVNILSDGTTAFTRDIANGGNSI
                     TEEIQRQLGIGRDEAESYKCGGEGRGIVPREVPEIVNQAVDQLAGEIQRSLDFYLATS
                     GDRDLSRICISGGTANIQALLDILQDRSRVPVEVLNPARVADVDEKAVGDLQGRAAQA
                     VVSIGLALRKERERN"
     gene            2929..3600
                     /locus_tag="VFG22_00670"
     CDS             2929..3600
                     /locus_tag="VFG22_00670"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HET6414775.1"
                     /translation="MIRINLLPTDKKKRARRMAPSPLPQGDFSLGTWGAIYGGAIAVW
                     LLVLGIFYVTQSGKIEVIAQENKTLEARRDELKSKTKGLAEVEESLEKSRRLEKVVEE
                     LERARRGPTRAVMELSKVLSSPGGPTINPAELERMREENPLAGYNESWDVRRLWLTRF
                     EEVDRECKIIGMGRSNEDVAEFLRRLTLSEIYESVTLQRTRAANDPDTGLQMVGFDIT
                     CKVKY"
     gene            3600..4181
                     /gene="pilO"
                     /locus_tag="VFG22_00675"
     CDS             3600..4181
                     /gene="pilO"
                     /locus_tag="VFG22_00675"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HET6414776.1"
                     /translation="MASLEDTFVGLPGWAKALGLVGVLALLSGAYYAVVHSSVSTDLE
                     AAQQQQKQLQDQIRDAERREKQYLELTQELANREVVDRQNRRTLPEDAEIAAFLQDLN
                     RVAELSGLTIRLVEPRPEQRQELYSKIPVVLALTGRFHQMTKFFYNVSKLDRAISMEN
                     IRMTQPKLLPSGEMLIDVDVRATTYRRPPAKGK"
     gene            4188..4811
                     /locus_tag="VFG22_00680"
     CDS             4188..4811
                     /locus_tag="VFG22_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414777.1"
                     /translation="MSSKIRNFACLLLVASIGFAGCGGDDEEFGGGLGLGSDQSGDSS
                     KKDSKKDTAEKSSSILESSPVVYADDDFVELDIQNRDPFRSYARAFKAQAAAPAQRRV
                     LLPSTSLDEMKLIAIVTGIATPRAMLTDTARVGHVIRAGDYIGRPEVVQSGGSEGMPV
                     TLNWRVDRIRPGSVVLTREDPSAPDKPPLTRVMPLHEGGEAAQLTAP"
     gene            4893..7061
                     /gene="pilQ"
                     /locus_tag="VFG22_00685"
     CDS             4893..7061
                     /gene="pilQ"
                     /locus_tag="VFG22_00685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HET6414778.1"
                     /translation="MRIHWGLAIAVGATFAWVNLAQAIGPKIDSVRINGERDEAQVSI
                     DGAFSDPQYTVQTRNDGRLIVIDVDNAALPANGIHTSGTNSLVSGTVASNTARGAHIE
                     LTLTGKATYHARASDAGIRIKIRRGSTPEHSRSKKASAPSSPKVEDIRIEQKDGRDRI
                     IVSLSGNPEFRVSTRAGALPRLEVLGAKIGENATRHVEAPHGALIGSIELQQKGDRAI
                     VEVHDSEGAAGTAIRSGDHIVWMFSPTTIDTRPQTRTIAREKDYAAEVDGPEVAGFLS
                     KLPMQAGQKNDRRYSGRRIDLDFKDADIHNILRLLSEVGGVNVVTSDNVGGTVTIRMR
                     DVPWDQALDVVLQAKSLGMVRQGNLLRVAPLAQLEQEREAAIARQKQQQQLAPLETRL
                     VPVSYATATNLQPRVRELLTERGTVSVDTRTNMLIVRDIVGQLDDVEDLVRNLDTQTP
                     QVLIESRIVEAGSTYSRDIGIQWGGAAVMSSATGNPTGLRFPSDIGVAGGVPVDGAQT
                     NGLSPFNGTVNNPNFAVNLPAVTGSGAGGALGLTMGSLSGAVNLNVRLSAAEAAGSVR
                     IVSSPRILTLDNNEASISQGTLIPFSRVSAQGVNTAFQEAKLELNVTPHVTSDGAVAM
                     DVKITRNEPDFGRTGANGDPTILEREAVTQLLVDDGDTAVIGGIYVRNTGRNIDQVPF
                     FGDIPVLGVFFKRRRFREDRNELLIFLTPRIVNRALALPE"
     gene            7175..>7847
                     /locus_tag="VFG22_00690"
     CDS             7175..>7847
                     /locus_tag="VFG22_00690"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HET6414779.1"
                     /translation="MGAGKSTVAQALAEQLGTRALDLDAQVEKLAGRSIPEIFAERGE
                     SAFRGLEKEALMDLPSEVGVVSLGGGTVVDDETRQALLRDGILITLTADPAVLASRVG
                     KGEGRPLLGDDPRGNLGRILRARAAAYAEAHAVLDTGSLEPQEIAAEIAGIRNLAPIV
                     VPLGLRTYRVEVGRGTRHRIGIRASEHADGDAIVVFDGGADRPWPAEAIRDLTLAGKP
                     PIEVKL"
BASE COUNT         1619 a         2328 c         2438 g         1462 t
ORIGIN      
        1 tccgggagct cgaacatctc ttgataaacg ccggcgtgtt cgcttcgggc ggtacgatcg
       61 aggcggagga tctcgcgatc gagagtggaa gagtgacgca atcgccgagc gcgggagcga
      121 gcaactacgt cgctttcaaa gagaccgaac gggatcggat tatcgctacg ctcaatgcgc
      181 acggctggaa tcgcgcgaag gctgctcgct ctctcggaat ggctcgccgg acgttctatc
      241 gccgcttgaa ggagcacgca atcgagctcc ctacatcgac tggcacgcgc gactaaaggc
      301 ttgctggctg gaccgagctc gatacgccag cgtcgtactt ttcgggcgtc tcgccgggcg
      361 gaagatagag tggcgcctca cccgggcgtg accaatccac ccagtagacc gccttcgacc
      421 ggacgtcgat gtggacgaag ctgctgcgtg ggtagtagcc cactcccacc ttggaaagcg
      481 tgaggcagta atcgcgcaga acttcattgg gcacgccttc gacccggatg tcgagcgcct
      541 cgcccgtccc gtgccgactc gacccagcgt cgctattctt gtcagcgcga tacgcactca
      601 ccacgacgat tgtgcgaccg ttgaagtggt cggccaggta tgtcagcgca cgcatcaggc
      661 gcacattggg gagcttcaca tcgtcggtct cgcgatcgcg cagaaatcgc ccgagacgcg
      721 cacgagcctt ggcgtccaat tttccccgct catcgaaaag atggacgcga agggtctcgt
      781 tgcttgccgg ccgttctagc gtcaccaggc cgtggagcac ggtgttttgc gctttactgg
      841 ccttggcgtt cgcactgtac cccggaagct ccagtcgctg tccgacgcga agggtggact
      901 ccggtttgag accgttggcc cttgcgagcg cttccttgct caccttgttg agttgagcga
      961 ttccggtcag ggtttggccc ggatacacgt agatcacgcc tttcggaggg atcctcagaa
     1021 cctgacccac gcacaggttg gaggtcttct tcaagctgtt cgcagccgca agattggtca
     1081 cggagacccg atatcgctga gcaatggcgc ccaaagtctg gcccgtctgc acggtgtgtt
     1141 tgcgctgggc atgagcctgt gcgctcagaa agagcacgag aaaaaccacg gcgaccccac
     1201 gccagacccc agttgagctc atcacgaccc gacgagtatt gatgagtccc ggggcagggc
     1261 aactaagcgg cgtggatccc ggtttttttg ccatcggctt ccagcattgc gaccatgaat
     1321 tcagacacct tcgttcgttg acaatcggtg ggttcgcgcg ttgagcacaa ggagtgccta
     1381 acgatcgcgg acgtctgtgt tccgcttgct aggttcactt aacttatata taattagcac
     1441 atgatttagt gctaaatttt cctaagttgc ttgaccctag tgtggttcgg tcatagcctt
     1501 gttgaggaac gacggagagt gcgttcctcc tccagagagc caagtcatga cctacgatcc
     1561 agaggcaaat gaagcgatgc aaaagcccga caccatgggt gcatcggcct ctgaagcgaa
     1621 cgatcagcaa agggcttcaa agcccgttgc gaacaagaag agtggcaaca ggcccaacaa
     1681 tgttcgcaag attcggatcg aacgcatgat gtccaaggcc gagttggctc gacgcgcgaa
     1741 cctatcggtg ttgacgattg atcgtgtcga gaagggtttt ggatgtcgga tggacacaaa
     1801 acgaaagatt ctcgaagctt tgggtctgag gctcgccgac agggtacgtg tgttcggcga
     1861 ggaggagtag cgtgtctaca tgccgtccga ggggaagaac ttaatcggcg tcgacatcgg
     1921 ttcgagctcg attaaagtct gtgaaatcaa agaagggcgc cggggggcac gaaccatcac
     1981 caggttcggc taccaccctc tgcccgccga aaccatcgtc gatggtcaca tcatcaactc
     2041 cggcgctgtc gtcgaggggc tcgagaagct ctttgcgcgt tcgaagcgac gcaatgtcgc
     2101 gcttcgcgcc agcggtcaca gcgtgatcat caagaagatt gcgatgccct tgatgacggc
     2161 tgcagagctc caggagcaga tcaactggga ggccgaacag cacatcccat tcgatctcga
     2221 tgaggtgcac gtggactacg aggtcctcca cgagcgtacc gaacacggtc agatggatgt
     2281 gcttctggta gccgcgaaga aggaagagat taccgatctc accaacctag cgatggaagc
     2341 tcgactgcgg cccatcgtcg tcgatctcga cgctttcacg gtccagaacg tgtttgaagc
     2401 cggttacggg gcagccgcaa gcgatgagac gacggtcctg gtccattgcg gagcgtctac
     2461 cacgaccgtc aacatcctct ccgacggaac tacggcgttc actcgtgaca tcgccaacgg
     2521 cggaaactcg atcaccgagg agattcaacg ccaactcggc atcggccggg atgaggcgga
     2581 atcgtacaag tgcggcgggg aagggcgcgg catcgttccg cgtgaggttc ctgaaatcgt
     2641 caatcaggct gtcgaccaac tcgccggtga gattcaacgc tcgctagact tctatttggc
     2701 caccagcggc gaccgcgact tgagccgcat ctgcatttcc ggcgggaccg ccaacatcca
     2761 agcactcctc gacatactcc aagaccgatc ccgggtgccc gtcgaggttc tgaacccggc
     2821 gcgggtcgct gacgtggacg agaaggcggt tggagacttg caaggccggg cggcgcaagc
     2881 ggtggtcagc atcggactgg ctctgcgcaa agagcgggag cgcaactaat gattcgcatc
     2941 aacctgctgc cgactgacaa gaagaagcgc gcacgccgaa tggcgccttc accgcttcct
     3001 cagggggact tcagtctcgg gacctggggt gcaatctacg gcggcgcgat cgccgtctgg
     3061 cttctggtgc tcgggatctt ttacgtcacc cagagcggca agattgaggt gatcgcccag
     3121 gagaacaaga ccctcgaggc gcgtcgcgac gagctcaaga gcaagaccaa gggacttgcg
     3181 gaagtcgaag agagtctcga gaagagccgg cgcctcgaaa aggtcgtcga agagctcgag
     3241 cgagcgcggc gaggtccaac tcgggccgtc atggagctct ccaaggtgct cagcagcccc
     3301 gggggtccca caatcaaccc cgcggagctc gagcggatgc gggaggaaaa ccctcttgct
     3361 ggctacaacg agtcctggga cgtgcggcgg ctttggctga cgcgcttcga agaggtcgac
     3421 cgtgaatgca agatcatcgg gatggggcgg tccaacgaag atgttgccga gttcttgcgt
     3481 cggctgactc tcagcgagat ctatgaaagc gtgacgctcc aacgaacgcg cgccgccaat
     3541 gacccggaca ccgggcttca gatggttggc ttcgatatca cttgcaaggt gaagtactga
     3601 tggcgagtct cgaagataca ttcgttggac tgccgggctg ggccaaggcg ctcggtctag
     3661 tcggagttct ggcgctgctg agcggtgcgt actatgcggt cgtgcacagc agtgtctcga
     3721 ccgacctcga ggcagcgcag cagcagcaga aacagctcca ggatcagatt cgcgacgccg
     3781 agcgtcgtga gaagcagtac ctggagctca cgcaggagct tgccaatcgc gaggtcgttg
     3841 atcggcagaa ccgtcggacc ctccctgagg acgccgaaat cgcagccttc ttgcaggacc
     3901 tcaacagggt cgccgagctc agtggtctga cgattcgctt ggtcgaaccg cgacccgagc
     3961 aacgccagga gctgtactcg aaaatcccgg tcgtgctggc gctgaccggg cgtttccacc
     4021 agatgaccaa gtttttctac aacgtgagca agctggaccg ggcgatcagc atggaaaaca
     4081 ttcggatgac ccagcccaag ctcctgccat caggagagat gctcatcgat gtcgacgttc
     4141 gcgctacgac gtaccggcgc ccgccagcca aagggaaata gccagccatg agctcgaaga
     4201 ttcgaaattt tgcctgcctg ctactcgttg ccagcattgg tttcgccggc tgcggtggag
     4261 atgacgagga gttcggcggc ggcctcgggc tcggttcgga tcaaagcggc gactcttcaa
     4321 agaaagattc gaagaaggac actgctgaaa aaagcagttc cattctcgaa tccagtccgg
     4381 tcgtgtatgc cgacgacgat ttcgtggagc tcgacattca aaatcgagat ccattccgtt
     4441 catacgcgag agcgttcaag gcgcaggcgg cggctcctgc acagcgtcgc gttctattgc
     4501 cgagcacgag tctcgacgag atgaagctca tcgcgatcgt caccggcatc gctacgccgc
     4561 gcgcaatgct caccgataca gcgcgcgtgg gccacgtgat ccgtgccggc gactacatcg
     4621 ggcgtcccga agtggtacag tccggaggtt cagagggaat gccagtcaca ctgaactggc
     4681 gtgtggatcg catccgtcct ggttcggtcg tgttgacccg cgaagaccct agtgcaccgg
     4741 acaaaccacc gttgacccgt gtcatgccgc ttcacgaagg cggcgaggca gcgcagctca
     4801 cggctccctg agtctcaaag ttaatcgacc gttttcttgc gccggcacgt cccggcgttc
     4861 gatgattaac gcagctggca gaggagtcag gaatgcgtat tcattggggt cttgcaatcg
     4921 ccgttggggc aacgttcgcg tgggtcaatc tggctcaggc gatcgggccg aaaatcgaca
     4981 gcgtccgaat caacggcgaa cgtgacgagg cacaagtctc tatcgacggt gcgtttagcg
     5041 atccgcaata cacagtgcaa acgcgaaacg atggtcgact gatcgtgatc gacgtcgata
     5101 acgcggcgct gccagcaaac ggcatccaca ccagcggcac caactcactc gtctcgggta
     5161 ccgtagccag caacacggct cggggcgcgc acatcgagct gacgctcacc ggcaaagcga
     5221 cgtatcatgc tcgcgcatcc gacgcgggca tcaggatcaa gattcgccga ggttccaccc
     5281 ctgagcattc ccgctcgaaa aaggcatccg ctccgagctc cccgaaggtc gaggacatcc
     5341 gtatcgagca gaaggacggc cgagaccgca tcatcgtttc tctttcaggc aaccctgagt
     5401 ttcgtgtgtc cacccgtgcg ggggcgctgc ctcggctcga ggttcttggc gcaaagatcg
     5461 gcgagaacgc cacgcgacac gtcgaagcgc cgcatggggc cttgatcggc tcgatcgagc
     5521 tccaacaaaa gggcgaccga gcgatagtcg aagttcacga ttccgaggga gccgcgggaa
     5581 cggcgattcg ctcgggagat cacatcgtct ggatgttctc gccgacgacc atcgacacac
     5641 gtccgcagac caggacgatt gcgcgcgaga aggactacgc ggccgaagtc gacggccctg
     5701 aggtcgcagg cttcctcagc aagcttccga tgcaggccgg gcagaagaat gatcgtcggt
     5761 actcgggccg gcgcatcgac ctcgacttca aggacgcgga cattcacaac atcttgcgcc
     5821 tcctttccga agtgggtggc gtcaacgtcg tgacctcgga caacgtcgga ggtacggtca
     5881 cgattcgcat gcgtgacgtc ccctgggacc aggccctgga cgtggtgctt caggccaaat
     5941 cactcggcat ggtgcggcaa ggaaaccttc ttcgtgttgc gccgctggct cagctcgaac
     6001 aagagcgtga agccgcgatc gcgcgtcaaa agcaacagca gcagcttgcg ccgctcgaga
     6061 cccgcctggt gccggtgagc tacgcgaccg ccacgaatct tcagccgcgc gttcgagagc
     6121 tcctgaccga gcgcggcacc gtatcggtcg atacgcgcac gaacatgttg atcgtccgcg
     6181 acatcgtcgg gcagctcgac gacgtcgagg acctcgtccg aaatctagat acgcagacgc
     6241 cgcaggtgct gatcgaatcg cggatcgtgg aggcgggtag cacctactcg cgcgacatcg
     6301 gtattcagtg gggtggtgca gctgtcatga gcagtgcgac cggcaatccg accgggcttc
     6361 gcttcccttc ggacatcggc gttgctggtg gcgtccctgt tgatggagcg cagacgaatg
     6421 gcctttcgcc cttcaatggg accgtcaaca atccgaattt cgccgtgaat ctccccgcag
     6481 tgacgggcag cggcgccggt ggcgcacttg gattgacgat gggaagcctg agcggcgccg
     6541 tgaacctcaa cgttcgtttg agcgcggcag aagcagctgg ctccgtgcgc atcgtcagct
     6601 cgccgcgcat tctgacactc gacaacaacg aggcatcgat ctctcagggc accttgattc
     6661 ctttctcgcg agtcagcgcg cagggcgtca atacggcctt ccaggaagcc aagctcgagc
     6721 tgaacgtcac gccacacgtc acgagcgacg gcgcggtcgc gatggatgtg aagatcacgc
     6781 gtaacgagcc ggatttcggc cggacgggcg ccaacggcga ccccacgatt ctcgagcgtg
     6841 aagccgtcac acagctactc gtcgacgacg gcgataccgc ggtcatcggc ggcatctacg
     6901 tccgaaacac gggccgcaac atcgatcagg tgccgttctt cggtgacatt ccggttcttg
     6961 gcgtcttctt caagcgacgc cgattccgcg aggaccggaa cgagctcctc atcttcctca
     7021 cgccgaggat cgtgaaccga gcgctcgcac ttcccgagta ggtccccgta gaagggatac
     7081 tcagcgtcct ggggcgggtc gcgccgtcgg gttcgtgact gcggccctga cggcccgtat
     7141 gcttccgatc gtgcgtgttt acctctcagg gcccatgggg gctggaaagt cgaccgtcgc
     7201 tcaggcgctg gcggaacagc taggaacgcg ggcgctcgat ttggacgcgc aggtcgagaa
     7261 gcttgccgga cgctcaatcc cggagatctt cgccgaacga ggcgaaagcg cgtttcgggg
     7321 tctcgaaaag gaagccctga tggatctccc ttcggaagtt ggcgtggtct ccctcggagg
     7381 tggaacggtc gtcgacgacg aaacccggca ggcgcttcta cgcgatggaa ttctgatcac
     7441 cctcacggcc gatccggcgg tcctggcgtc acgcgtcggc aagggtgaag ggcgtccgct
     7501 cctcggagac gatccacgtg gcaacctcgg gcgcattcta cgcgctcgcg ccgccgccta
     7561 tgccgaagcg catgcggtgc tcgataccgg ctcgctcgag ccccaagaaa tcgctgcgga
     7621 gatcgctggc attcggaatc tcgcgcccat cgtagttccg ctcggcctgc gcacgtacag
     7681 ggtggaagtg gggcgcggca ctcggcatcg cattggtatt cgcgcgagcg agcatgctga
     7741 tggcgatgcg attgtggtgt tcgatggggg cgccgatcgc ccatggcccg ccgaggcgat
     7801 tcgggatctg acgctggcgg gcaagcctcc gatcgaggtg aagctta
//
LOCUS       DASRZF010000014        29634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_694455, whole genome shotgun sequence.
ACCESSION   DASRZF010000014 DASRZF010000000
VERSION     DASRZF010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 29634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29634
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694455"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..534)
                     /locus_tag="VFG22_00695"
     CDS             complement(178..534)
                     /locus_tag="VFG22_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414780.1"
                     /translation="MSLDAAARRRYARQLLLGEIGEAGQERLLASGFRSRAGGAADAF
                     AVAADYLERAGCFCDSEGAEVRIPRASEVMRFAGNAALREPAALVLGAYSAVEYLKEV
                     LGIAAASEVPTDLSLS"
     gene            complement(531..812)
                     /locus_tag="VFG22_00700"
     CDS             complement(531..812)
                     /locus_tag="VFG22_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HET6414781.1"
                     /translation="MAVTVRIPTPLRTLTGGADEVSVAGGTVAEVIEHLESNHPGMKD
                     RLCDEKGVRRFVNIYANDEDIRFLDNLETTLKEGDTLSIVPAIAGGAPR"
     gene            complement(852..1559)
                     /locus_tag="VFG22_00705"
     CDS             complement(852..1559)
                     /locus_tag="VFG22_00705"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HET6414782.1"
                     /translation="MSNELSVLVVGAGGLGSPVLRLLAQAGVAQITVIDDDIVDESNL
                     HRQTLYTSDDVGVSKIERAIRRLRDLSPGLHVRGIEGRFVPGTAMDLLARQDLVVEGA
                     DNLATKFLVTDAARLAGVPAVQAGAVRWAGWAFCALPDSACLRCLFEDIPTEQVETCA
                     QAGVAGPVVGVIGGLQAALALRFLRGERPGGELWHFDALRGSLRKSFVRRRSDCLLCA
                     GEIKDLRMERYTAACAA"
     gene            complement(1556..2482)
                     /gene="cysK"
                     /locus_tag="VFG22_00710"
     CDS             complement(1556..2482)
                     /gene="cysK"
                     /locus_tag="VFG22_00710"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HET6414783.1"
                     /translation="MSAAVVDSVLDLIGETPVVRLRSLSPKGGATIWGKCEHLNPGGS
                     VKDRICLAMIEAAEAEGRLEPGDTIVEPTSGNTGIGLALVCARKGYKLVLTMPASMSL
                     ERRALLEAYGVEIVLTAPDRVMEGAMEAARRIVKERGAYMPEQFSNPANPGIHRRTTA
                     SEILRAFEGETIDAFVAAVGTGGTVSGVGEALKRVNPATRVLAVEPSASPVLLGGRPG
                     PTKIQGLNAGFIPQNYHGEIVDEVIGVSDRDAWDTKLALAKQEGLLLGISAGANVWAA
                     IEVAKRLEPSQIVVTILCDTGERYFSLGEYFQ"
     gene            complement(2482..2934)
                     /locus_tag="VFG22_00715"
     CDS             complement(2482..2934)
                     /locus_tag="VFG22_00715"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET6414784.1"
                     /translation="MKLSNKGRYGVRAIFDIAFHSGGKATQIKDISRRQAIPPRFLEQ
                     IFQDLKRAGLVTSKRGPRGGYALAIDADDIRLGDIVRALEGPIMLANSDGNGDSDETS
                     RLVTENVFSELSENIEACFDAITIQNLCDRGDAHGVRRAPPRRYVYSI"
     gene            complement(2979..3974)
                     /locus_tag="VFG22_00720"
     CDS             complement(2979..3974)
                     /locus_tag="VFG22_00720"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HET6414785.1"
                     /translation="MKDKEIGILLGGVSAERDISLKTGEAMFEALKARGYKVQKVFVD
                     ADIDRVLRQVSIDVAVIALHGTYGEDGCIQGMLETMGIPYTGSGVLSSALAMDKLKSK
                     ELFRLYNVPTPSYYVVRADDLDRLEQIHSSFGFPSFVKPRSGGSSVGAGAARSLTELR
                     QRAEDAARFDQWILVERLIRGREVAVGLLDGNALGAIEIEPKGGFYDYKSKYQKGQSE
                     YHFPARLSPTRYQGVLNLAERAVQAVGASGATRVDLLVTSDENEYVLEVNTLPGMTPT
                     SLLPKIAAGAGYDFGDLCEAILERAGLQNGEGLPSVSAAERAAFGAPGIPITAAE"
     gene            4501..6252
                     /locus_tag="VFG22_00725"
     CDS             4501..6252
                     /locus_tag="VFG22_00725"
                     /inference="COORDINATES: protein motif:HMM:NF016924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET6414786.1"
                     /translation="MTFTEAAVEVLRSVGKPLHYKKITEIAIERNLLSHVGKTPETTM
                     SSRLATMVKKDRGDAPILKVKPGVFALREFPEDVVEDAEGVLADSDAPEALERDGADE
                     EPASFEEPAETEEESSLVVQDLPGSDVFPEEDDDDELILANLDDDSKDARKPRKRRPR
                     RRRDRDSDNGSSAESRDSRDSRDSRDSRDSREARDSRESRPDRRPPNLRGRPQREPVA
                     PATRAAEPGEPVGRDLSDAIETALRGGSRQARTLVQVAEALIDTGRLAGAPQLLAATL
                     GAAIRGDNAKRRASGGRPRFRELSGLVSLLEWDLPGDVVNAERDAVRAADRQRQSVRR
                     AFNKRLRDLPNGALLELLATWLNAVGVHSLHAVRANAGDFNLAGTLRRGPEETPLAIS
                     IYRGNQPIGNEAIVALRGGLHHFDHARIGWVITLGQARDGTLGEAHAEGAAPCAVFDG
                     DALAISMEEVGVGIQRASLPLAVLDLELLDSLAGGSARSSAPRSAPNNEESVDSEGNK
                     RRRGRRRGRRRGGRDENGEEARRDDFAEASETKESSDALDSERPEVEAVSDSSPEPAE
                     DKDKKGEEPQLAPELEA"
     gene            6461..7051
                     /locus_tag="VFG22_00730"
     CDS             6461..7051
                     /locus_tag="VFG22_00730"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HET6414787.1"
                     /translation="MIDYRKAVGDHPTSLSLARAIDARLTVRESREGDPIHVQHCRES
                     LGGCRARIATLSRLITEASNRAGVDPFLTAAIAVRESGLNPLAVSPVGARGVVQLNPK
                     YRGKTVPFVYNERYREKCSLRPGACQREVLEAGLGLYGWAVSRCGGDVAKGLAWYNSG
                     HCDKQNGYAASVLRERRRLLRLAKDTDPRLEAVFPN"
     gene            complement(7048..8211)
                     /locus_tag="VFG22_00735"
     CDS             complement(7048..8211)
                     /locus_tag="VFG22_00735"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET6414788.1"
                     /translation="MGVGLVEQLVSAGLVTAAQASASHAGDPLVPSGRVLRNLVTAGL
                     DERVLAGFFISRGFGPMLQEQDLRRADQELVRRLPASDAHDLCAMPLRPSPAGAIVAM
                     ADPTDAHAVARLGEALGGSILPTVAKLSDLLASIDRAYPPDRPTLVSDPLALARSRIP
                     SDAFALTHEKSEVGSQPTLSEFDPAFSGLASTASPVWDRAWHHSTTEREVPLPSISHS
                     PQRPATPGPAPASESNIELRLHELTQATSRDEVARLACEACLTAAQGAAFLALRKGVF
                     RGWDGAGDEVTSASIRSLWVPASNPSVLNEVLHTGRPFRGAYGQTAADHLFRAALGSR
                     GRDVVVVPVLVGTRMVGVLCANDPAPDAAPVERVAEAMGEALERLIVSQKSGI"
     gene            8276..9493
                     /locus_tag="VFG22_00740"
     CDS             8276..9493
                     /locus_tag="VFG22_00740"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HET6414789.1"
                     /translation="MARVLHIEDDPNNRRLVQKLLGAAGHEVIEAEGGVQGIQLAREI
                     LPDLVLVDINIPDLDGYEVTLRLRGTPALQDVPIVAITAEGDRDSTLAVGCDGFIAKP
                     IDAAHFAETIAEFLGGHRESADDGSDRLLRARTQKIVERLEKKIVELSETNRRLEDIA
                     RLRREFLQNVSHELATPMTPVVGYLRLLINEELGPLSDLQRKCLSSIESSTQRLRTVV
                     DTLLDVSSLETGRMHYYTRPYDFREVAMKALDQIRPKLVERDVTLTERIPGEPMPAQG
                     DPEKLLRAMVHVLDNASKFTPTGGEVAVEVRPEGEDHLLFAAADSGPGVRPEHIARIM
                     EPFYQVDGSVTRDHGGVGLGLAFARRVTEALGGGIEISSPPAGEVAKRQLSGTLVELR
                     VGRIPERPEIPSQ"
     gene            9490..10599
                     /locus_tag="VFG22_00745"
     CDS             9490..10599
                     /locus_tag="VFG22_00745"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HET6414790.1"
                     /translation="MIYLDHHAAAPTPEAVRRAMDAAAPAAWANPSSTHAAGRAARRL
                     LEAAREDVAKAVGAAPADLVLTSGGTAACNLGIRGIAAGCKRVLSTEVEHPAVAESVR
                     RLGDEGRERIVIAVREGQPPSGKELARHLGPGTLVAVQWVNHETGTVFPVHEYAEVCR
                     ERQALLFVDASQALGKLPIDLGTLGADAVALGAQKIGGPPGAGACWIRRGLDVLPVLV
                     GGSQERGRWPGTPDTLSMVGFGAACRLTGKRLEDQSRVTALRDRLEASLLGLGGVLNG
                     GCPRTGTVSNISFAGWEGPLLVAALDLEGICVSTGAACSSGMQEPSAIIRSMYPDEQW
                     RAGSAVRISLGIETSEQDIETALLVFRRVLGRKLA"
     gene            10764..11825
                     /gene="mnmA"
                     /locus_tag="VFG22_00750"
     CDS             10764..11825
                     /gene="mnmA"
                     /locus_tag="VFG22_00750"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HET6414791.1"
                     /translation="MGEVKRVLVAMSGGVDSSVAAALLRESGHELVGVTLHLWDADGE
                     AQVGRCCAPEDRDDARRTCEHLGIPHYVLDERAAFRKHVVDPFIAEYQAGRTPIPCVS
                     CNRTVKLSYLAEVARRFDATHIATGHYARLERGGDGQLRLLRGRDGDKDQSYFLFGLA
                     QSTLERLIFPLGDLSKSEVREAGRRLGVPNADKAESQELCFVPDGRVGDFINRQGNPS
                     PPGAIVDSEGGLLGGHQGVANFTRGQRRGLGLGGGRARYVLRIVPDTAAVVVGDESEL
                     YDDALSATDAHWVAGRPAEPFQGEVRIRYRHRGARADVIPTSDGFEVHFDEPQRAITP
                     GQAAVVYRGDEVIGGGFIA"
     gene            11852..13108
                     /gene="rlmD"
                     /locus_tag="VFG22_00755"
     CDS             11852..13108
                     /gene="rlmD"
                     /locus_tag="VFG22_00755"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HET6414792.1"
                     /translation="MPAKHLALGEVRAVGRGGDAVVETADGIVLVPGALPTERVEIAL
                     GSSKRGAARGILTRILRAAPGRREPACADALRCGGCPLMIANAEVQRQIKLGFLRDAC
                     RGLPGSDTTEIDWITSPAELGYRRRARLAWHGRNIGYRQLHSKRINDIAECIVLAKPL
                     REAWTELRACLAPSLQGEGEIHLQLTDTDRVLVDLRAEHDQLPNLFEACEALSHSPEI
                     AGVSLRTGETGHAASWGETQVRHLRAAGGSLEGPAGAFSQANDAVNAKLVEAVIELAE
                     PEGRRVLELHSGIGNFTIGLAELAKTLVAVEQDPQAVQACQSNLERRGLQARVTVGDA
                     NQPPKGKYEVIVLDPPRQGAKALFERSDLLPGPKRIVYVSCDTATLARDLRIATAKGY
                     RIERAIGFDMFPQTAHLESLVLLVHA"
     gene            13162..13611
                     /gene="nrdR"
                     /locus_tag="VFG22_00760"
     CDS             13162..13611
                     /gene="nrdR"
                     /locus_tag="VFG22_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HET6414793.1"
                     /translation="MRCPYCGTLENRVIDSRLSQAGEVTRRRRECEHCGRRYTTYERV
                     EQTLPLIIKTDGRRQPYDRDKLLAGLRRACVKRPVSAEALERFLHQVERWLVDTGEQE
                     VTSARLGEHVLHELRGLDPVAYVRFASVYRDFQNVGEFLDELAKLKD"
     gene            13613..14716
                     /gene="ribD"
                     /locus_tag="VFG22_00765"
     CDS             13613..14716
                     /gene="ribD"
                     /locus_tag="VFG22_00765"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HET6414794.1"
                     /translation="MWSDFEREVMARAIAEARRGRTSPNPRVGAAIVRNGKLVSLGHH
                     AKAGEAHAEVDAIRNARGSVEGAALYVTLEPCNHHGRTGPCTEAILKAGIAKVVVGCR
                     DPAPHVAGALDRLRSAGLEVELGLMERECTALIADFAKHIRTGLPFVTLKAAVTLDGK
                     IATRSGDSKWITGDEARAEAHRMRDESDAILVGVGTVLADDPKLTVRHVEGRNPVRVV
                     LDANLRTPKTAAALKQDKASKAEVLIFHAHDANVERRESLRGPGIELIPVARHARGVD
                     LVEVLKLLGERGFVRLLVEGGAHVHGTFLDLHLADRAAIFIAPRIVGDANALSFALGD
                     GVDTMEQASRLVRTEIQTFGTDWLISGDFERTH"
     gene            14717..15322
                     /locus_tag="VFG22_00770"
     CDS             14717..15322
                     /locus_tag="VFG22_00770"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HET6414795.1"
                     /translation="MFTGLIEQLGTIEDLDQREDGLSIRIGCALHPFELGESIAVNGT
                     CLTVKSFSERHFDADASNETLDKTNLGHLSVGDKVHLERALALGERLGGHLVTGHVDG
                     VGTLSGKTPDGDYLRASFEVPTRLAPFLAPKGSITVDGVSLTVNTVSGSRFDVMLVPY
                     TLDETTFGEMPMGAKVNLEVDILAKYVASLMGKPGVDAIED"
     gene            15379..16533
                     /gene="ribB"
                     /locus_tag="VFG22_00775"
     CDS             15379..16533
                     /gene="ribB"
                     /locus_tag="VFG22_00775"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HET6414796.1"
                     /translation="MTSAIERVVAALDDIRKGKMVILVDDEDRENEGDVCMAAEMASP
                     EAINFMARHARGLICLSLTEDRIRQLDLPMMVDDNRSSRSTAFTISIEARRGVTTGIS
                     AGDRAHTILTAVADDAGAMDLVSPGHVFPLKAVPGGVLQRTGHTEGSVDLSRLAGLKP
                     ASVICEIMKDDGSMARYPDLVAFAEAHQLRLLTIADLIQYRLARERLVTRIRQTPVKM
                     PTGRTWTASVYQVQVGGQAQFMALSLGEMSAEPTLVRVHRGSVLGDAFQVRMGARVHI
                     ADCVAAIERKGNGAILFLPGHPDPETDLAFYLGEPIPRPPEEPGVVLREYGFGAQVLS
                     DLGLEQLLLLTNRPRRIPSLDAYGLTVVDQLMVAPGGELSSASEQTRDET"
     gene            16530..17036
                     /gene="ribH"
                     /locus_tag="VFG22_00780"
     CDS             16530..17036
                     /gene="ribH"
                     /locus_tag="VFG22_00780"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HET6414797.1"
                     /translation="MSDDRNQPLTEVEAPANARFAIAWSRFNHHVVSRLLDGAKACFS
                     EHGLSESSIYVVEVPGAWELPHAAQKLAESGCFDAVLVLGAIIRGGTPHFDYVSQGTA
                     LGIGRVALDTGIPVVFGVLTTDDETQALERAGGAHGNKGRDAALTAIEMAAFARKLVN
                     DGIKTKRA"
     gene            17011..17430
                     /gene="nusB"
                     /locus_tag="VFG22_00785"
     CDS             17011..17430
                     /gene="nusB"
                     /locus_tag="VFG22_00785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HET6414798.1"
                     /translation="MGSRRKGREAALRILYFMDVSGVSGTQAIKSYWGHLASEEERTE
                     DVDFANRLVRAYADRQDEVNDLIRSSSHHWRLERMPIVDRNVLRVAIVELRELNDIPK
                     RVTLNEAVELAKRFGSEGSGSFVNGVLDRIATELGKP"
     gene            17427..17954
                     /locus_tag="VFG22_00790"
     CDS             17427..17954
                     /locus_tag="VFG22_00790"
                     /inference="COORDINATES: protein motif:HMM:NF014808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HET6414799.1"
                     /translation="MIAYDFTPASARGLDELQTQLLVLPFFSDERPLRGPAGLVDWRL
                     CGALSRKLMSGYIKGSFGEKALLTTPAKLQADGLLLFGLGESSDFSTRIAEKACLLIG
                     DALTEAKVSTAALALPGRSMRLLSALEAMQIWLDCSDTARELEELSVIEEGPAHRSLD
                     SLLDGLRRQAESPLD"
     gene            complement(17951..19333)
                     /locus_tag="VFG22_00795"
     CDS             complement(17951..19333)
                     /locus_tag="VFG22_00795"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET6414800.1"
                     /translation="MDTEEFTVSSVIPASPNRIYSAWMDERHHSAFTGARATVDPWVG
                     GRVVTDDGFIDATHVYLDTGSRIAMTWRTRDFPKGSNDSQVDITFEPVAGGTKVEVTH
                     SKIPAGLGKACEEIWNKLYLKPMARYFGEPDAMRAAYTAAKKAGHLPLSGIKASRPAP
                     RRPFIKAPPPPIEDSEDEEEEEKPKKTRARFESKTIDLRELALAQAEARKEAARRKAE
                     TKQKAEAARRQAEAAVKKAEAKKKAELMRIKKAEAKRKAAEKKKAELERKAAAAQKKA
                     EAKKKAEEIKQKKAAAKKVADELKRKKAAAKKKAEEIKRKNAAAKKKAAAKKKAAAKK
                     KAAAKKKAAAKKKAAAKKKTSAKGRGTPTKRKATQQKRGAKKKRTSAKKKVAPKKRSG
                     TQKRSAAKKKVAPKKRSVTKKRSAAKKKSAPKKRSTAAKRRAVPAKRRPTKKRSKTSA
                     KKAKRRSSRR"
     gene            19533..23072
                     /locus_tag="VFG22_00800"
     CDS             19533..23072
                     /locus_tag="VFG22_00800"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HET6414801.1"
                     /translation="MEEPTPRHQFWIDRGGTFTDCIHRDLESGALRVTKVLSSDQAPL
                     EGIRALLGLGPSDSIPASEVRMGTTLATNALLERGGRPTVLIADQGLGDLIRIGDQTR
                     PDLFALEIEKPEPLPAWVIEIGARMNPDGSIFRELDESELRNALEEARNSGAESVAIA
                     LMHAYRDGTLEGRVGAIAKEVGFAHVSTSNEISNEIGLLGRADTSTANAYLTPLLTQY
                     MAALEKELGSSRLRMMQSNGGLVDASGFIGRNAVLSGPAAGVVATGAIAEELGLSEVI
                     GFDMGGTSTDVSRFAGQVSRVFESQIGGIRIRAPMMELHTVASGGGSICKLEGERMIV
                     GPQSAGSDPGPLCYGNSQAEELTLTDVSLCLGRLSADRFPFPLDVERAKKRLAAVAAS
                     LRATGVERSAVEAAHGFLRVAVENMATAIQKISVGRGHDVRNHAMVVFGGAGGQYACL
                     IARRLGIRDLVFHPYAGALSAFGMGVANFEWHGERDAGRADLAAPLPTGWERAFEDLE
                     ASGRAALARDAGAQARWNVRRRVALHYRGTETTLTVDWTDAHTMRADFEVLHRREFGY
                     VRPQHAVHAATLRVQVELSSPKPDLPRVEAGSGAALRTTMLWSDGALVEAPVYLREDF
                     PLQTEVRGPAMILDATGTVVVDSGFVAIRDERDYLTMRDVESRIEREMADTRVDPVLL
                     EVFNSQFQSIAEQMGAVLQRTAMSTNIRDRLDFSCAVFDVTGGLVANAPHIPVHLGAM
                     SESVRAVLHAHPDMKPGDVFVTNDPFAGGSHLPDITVVTPQHDEKGVLRFVVASRGHH
                     ADVGGLTPGSMPPASTRLDQEGVVLRTLRIVEDGAFREQLLREALGQGRYPARNPDEN
                     VADIQAEIAANEKGLQLLDDLLARYGLEVVIAYMQHIQDNAAALTSRAIADLPDGEYR
                     FEDQLDDGARILVCVTVDGGEMSIDFEGTDRALDNNLNAPRAVTMAAVLYVLRVLVGK
                     PIPLNGGCMRPVSVRIPEGSLLCPEPGHAVAAGNVETSQRVVDVLFGALGLAAASQGT
                     MNNITFGDDRFAYYETLGGGAGATARRPGADGVHTHMTNSKCTDPEILETRFPVRIRR
                     FSLRHGSGGRGRLRGGDGLIRELEFLAPMRVSVISERRLVAPYGMRGGGSGATGMNLL
                     NGKQIAHRVTVQVAPGDILRVETPGGGAWGESE"
     gene            23069..23653
                     /locus_tag="VFG22_00805"
     CDS             23069..23653
                     /locus_tag="VFG22_00805"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HET6414802.1"
                     /translation="MNLKDLWRRLQQIVVHNILHLDDTPHRIAWGVFIGAIIAFTPTL
                     GLQIVIYLPLAALLRANKVSGIPILFISNPFTAVPLYYATWSVGAAVLRPEKEVTRAT
                     IKTWLGNMGRALKDGGVRRLLDTEFWSDAGRLLATTGGELWIGGLVCGLAVALPAYFL
                     TRWGINAVRHLREGRHLPHGPIDRKSNPKLKSEG"
     gene            complement(23691..23942)
                     /locus_tag="VFG22_00810"
     CDS             complement(23691..23942)
                     /locus_tag="VFG22_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414803.1"
                     /translation="MTEQQKMQSAVEENVNAAVGQVTKTAEDALSMGRKVTALWLGVT
                     RSAIEAAAKTLSATAEVISTLAESMGELAKRVEGSAKNV"
     gene            complement(23935..24474)
                     /locus_tag="VFG22_00815"
     CDS             complement(23935..24474)
                     /locus_tag="VFG22_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414804.1"
                     /translation="MGQDEESKSRRAKKERVLHTRVPAVLEQELKRVANAWRVPVSNV
                     VRALLEDALDTLDVVGAKAEGELREVAELLASERHRFRKKSAEQLSRIPGASDRTEPP
                     EGPYGATAERQADPLEGAVGVTPIILVHDAVCGVTGATLAAGSEAYMVLFDDPRRKLI
                     TGREALPQAPTSNEEKAHD"
     gene            24579..25196
                     /gene="lspA"
                     /locus_tag="VFG22_00820"
     CDS             24579..25196
                     /gene="lspA"
                     /locus_tag="VFG22_00820"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HET6414805.1"
                     /translation="MTRKHRSSKAFVLCLVAAALLAAMDLGSKHWALDRLSQPSAQGA
                     EPVCEPNEYGRTEPQRAQRSPIVLIDGFLEFRYAENCGAAFGFMRDMPSLVRKGVFYV
                     AATGAIVLLLWMFVTGRGGALFAISVPLIVAGAIGNLVDRVRLGYVVDFVRFHIHDRW
                     AYPTFNIADAWITIGVALILIEGFVDGRREKQERAQNTEGSAGET"
     gene            complement(25209..28406)
                     /gene="ileS"
                     /locus_tag="VFG22_00825"
     CDS             complement(25209..28406)
                     /gene="ileS"
                     /locus_tag="VFG22_00825"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HET6414806.1"
                     /translation="MFDPVSPQVSFPELDATQLAFWESARIFEKASAGGKDKQPFIFY
                     EGPPTANGMPHPGHVLTRVMKDVFLRYRTMCGYEVPRRAGWDTHGLPVEVEVEKELGI
                     SGREAIEAYGVEAFSRKCIDSVFKYIGEWQKMTERIGFWVDFDSAYVTFHKSYVESVW
                     WALSEMFNQGLLYQGYKVVWWWPQGGTALSAGEVGQGYQTIDDPSVMIRFPVLGETRT
                     SFLAWTTTPWTLPSNIALAVDAKTDYATVELDDGERLVLAEALVSKVLGDDAKIVATR
                     KGAELVGMRYEPPFRYADPEGGPAWQVVDADFVSLDSGTGLVHLAPAFGEDDFRICKE
                     KGMGFLQLLKPDGTFPEEVTDFAGRFCKDADRDIIRNLRARDVLFKEEAYRHDYPFCW
                     RAMQDPLIQYARRSWFIRTTQEIGRVKENNQRVHWEPEHIQNGRMGQFLEGNVDWALS
                     RERFWGTPLPIWMNDETGAVEAIGSVAEILQRNPDAFAHFEKAKAADPSLQDHLMVHK
                     PWVDEVTWARDGEPGVYRRVPEVIDCWFDSGCMPFAQWGFPHQNSEGFERSFPADFIT
                     EAVDQTRGWFYSLMTISSLMFPERALPHPFKNCVVLGLMTDEKGKKLSKRDKNYTDPL
                     VLMDRVGADAVRWALYSGTVPGQNTRFSDDAATDAVREFLLKIWNVYSFFVTYANIDG
                     WSREDERPMLAERPDMDRWVLAELDATVRAVRQELDEYKSHMAVRHLLELVDGVSNWY
                     VRRSRARFWASEDSFDKRAAFGTLYEVLVDLTKLAAPFIPFMTETLFQNLVRQGDATA
                     AESVHLAAFPEPQEARVDDELRQTMRRVRNLVTLGQRVRNENKLKVRQPLAEAIAVVA
                     DDAERLAIDRFSSAIREELNVRELGFTLEPHKYVEFELVPNFRVLGPKLGKQVPACKK
                     ALSEADGSALYVEMEQNSKIVIELPDGPIELTPDEVEVRLSAREDFAAASGAGQVVVI
                     DTRVDESLRREGLAREVINRIQRARKAMDLAYEARIEVSWNAEGELAAAIEENASRIA
                     TETLATAFGRTATPEHSEHDTEIEGAPLSLAITAA"
     gene            28493..29344
                     /gene="folD"
                     /locus_tag="VFG22_00830"
     CDS             28493..29344
                     /gene="folD"
                     /locus_tag="VFG22_00830"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HET6414807.1"
                     /translation="MTELIDGKALAKEVRAEVKARAEAFKATHNRAPGLDVVLVGEDP
                     ASEVYVRNKERAAAKAGIQGRVHRLPAQTSEADLLALIFELNGDDEIDGILVQLPLPD
                     HLHDQTVIDAIDPAKDVDGLHPFNAGLLVVGRKGLRPCTPSGCMRMLERVGCDPNGKR
                     ALVLGRSTLVGKPIALMLLEKHATVTIAHSRTKDLADRVRESDIVVAAVGRPNLVQGD
                     WIKEGAVVIDVGINRLEDGTLTGDVEFEGAKQRASYITPVPGGVGPMTIAMLLSNTVD
                     AAEARIL"
BASE COUNT         5406 a         9328 c         9322 g         5578 t
ORIGIN      
        1 cgagcatcat ttggcgagcc ggccggtctt tgaccgagcc tccggggttg gcgtattcga
       61 gcttcagata gatcttcgtg ccgggcgcat ccttttcgag cgatcgcagc cggacgagtg
      121 gtgtattacc cacggcctcg acgatcgatt ccatcttgcg aaagcgcatc tgatctctca
      181 gctcaagctc aagtcggtcg gcacctcact cgcagctgcg atgccgagaa cctccttgag
      241 atactcgacc gcggagtagg cgccgagcac cagggccgca ggctccctga gggcagcgtt
      301 accggcaaag cgcatcacct cgctcgcccg aggaatacga acctcggcac cttcggaatc
      361 gcaaaagcat cccgctcgtt cgagataatc cgctgccacg gcgaatgcgt ccgctgcgcc
      421 gccagccctg ctgcgaaacc ccgaagcgag caggcgctcc tgtcctgcct ctccgatctc
      481 accgagcaga agctgacgcg cgtatcgacg ccgtgccgcg gcgtctaagc tcaacgtggg
      541 gctccgcccg caatggcagg aacgatgctc aatgtgtcgc cctctttgag tgtggtctcg
      601 agattgtcga gaaatcgaat gtcctcgtcg ttcgcgtaga tgttcacgaa tcggcgcaca
      661 cccttttcgt cgcagaggcg atctttcatg cccgggtgat tggattcgag atgctcgatc
      721 acctcggcca ccgtaccgcc ggccacggag acttcgtcgg ctccgccggt cagagttcgg
      781 agtggggttg gaatgcgtac cgtaacagcc atgttcgttc ctttctgagt tccaccaaat
      841 gcgagcttgg gtcaggcagc gcaagccgcc gtgtacctct ccattcgtag gtccttaatc
      901 tctcccgcac agagcagaca atcgctccgg cggcgcacga atgacttgcg aagcgaaccg
      961 cgcagcgcgt caaagtgcca aagctccccg cccggtcgct cgcctcggag aaagcgaagc
     1021 gccagagcgg cttgcagccc accgatcacg ccgacgacgg gtccggccac tccggcctgc
     1081 gcacaggtct ccacctgttc ggtcggaatg tcttcgaaga gacatcgaag gcacgcggag
     1141 tcgggtaggg cgcaaaaagc ccatccggcc cagcgaaccg cgcccgcctg aaccgccggg
     1201 acgcccgcaa gacgtgcagc atcggtgacg aggaacttcg tggcgaggtt gtcggcgcct
     1261 tccacgacga ggtcctgccg cgccagcaga tccatcgccg tacccgggac gaatctgccc
     1321 tcgattccgc gcacgtgaag tccgggcgac aggtctcgga gcctgcggat agcccgctcg
     1381 atcttggaca cgccgacatc gtcgctggtg tacaaggtct gccgatgtag gttgctttcg
     1441 tcgacgatat cgtcgtcgat gaccgtgatc tgagcgacac ctgcttgggc aagcaggcgg
     1501 agcaccgggg atccgagccc gccagcgcca accaccaaga ccgagagctc gttgctcact
     1561 ggaagtactc ccccaagctg aagtaacgct ccccggtgtc gcagagtatg gtcaccacga
     1621 tctggctggg ttcgagtcgt ttcgcgacct cgatcgcggc ccagacattt gcgcccgcgc
     1681 tgatgcccag aagcaagcct tcttgtttgg cgagggcgag cttggtgtcc caggcgtcgc
     1741 ggtcgctcac gccgatgact tcgtcgacga tctcgccatg gtagttctgt ggaatgaatc
     1801 ccgcgttgag cccttgaatt ttagttggtc cgggccgtcc gcccagcagc acggggctcg
     1861 cggaaggctc cacggcgagc acgcgcgtcg caggattgac ccgcttcagt gcctcaccta
     1921 cgccgctcac ggttccaccc gttcccaccg ctgccacgaa cgcatcgatc gtttcacctt
     1981 cgaaggcgcg cagtatctcc gacgcggtcg ttcgcctgtg gatgccggga ttggcgggat
     2041 tcgaaaactg ctcgggcatg taggcgccac gctctttcac gatccgtcga gcggcttcca
     2101 tcgcaccctc catgactcgg tcgggcgcag tgagaacgat ctcgacgccg tacgcctcga
     2161 gcagagcccg gcgctcgagc gacatgctcg ccggcatcgt gagcacgagc ttgtagccct
     2221 tgcgcgcaca gaccagcgcc aagccgattc cggtattgcc gctcgttggc tctacgatcg
     2281 tatcgcccgg ctcgaggcgt ccttcggcct cggccgcctc gatcatcgca aggcagatgc
     2341 ggtccttgac ggagccgccc gggttgagat gctcgcactt gccccaaatc gtcgcgccac
     2401 ctttgggcga aagcgatcgc agacgaacga caggcgtttc gccgatgaga tcgaggacac
     2461 tatcgacaac cgctgcggac atcagattga atatacgtaa cgtcgaggag gcgcgcggcg
     2521 aactccgtgg gcgtcccctc gatcacagag attctggatc gtgatggcgt caaagcacgc
     2581 ctcgatgttt tcggatagct cggagaacac gttttcggtc acgaggcggc tggtctcgtc
     2641 agaatcaccg tttccgtcgc tgttcgccag catgatcggc ccctcgagag cgcgcacgat
     2701 gtcacccagc cggatgtcgt cggcgtcgat ggcaagcgcg tagccgcccc ggggaccgcg
     2761 tttggacgtg accagacccg ccctcttgag atcctgaaaa atttgctcga ggaagcgagg
     2821 gggaatggcc tgtcggcgcg aaatgtcctt gatctgcgtt gccttgccac cgctgtgaaa
     2881 cgcgatgtcg aaaatggcgc ggacgccata tcggccttta ttcgagagct tcaccgagtg
     2941 ggatctatgg tttcccacat cggaattgtc aacaatgact attccgctgc tgtgatcggt
     3001 atgccggggg caccgaatgc cgcacgctcg gctgcggaga cgctcggcag tccctcgccg
     3061 ttctgaaggc cagcgcgctc gagaatggcc tcgcaaaggt cgccgaagtc gtatccagcc
     3121 cctgctgcga ttttcgggag caacgaagtg ggggtcatgc cgggcagggt gtttacctcg
     3181 agtacgtact cgttctcatc ggaggtcacg agcaggtcca cccgcgtcgc tccgctcgcg
     3241 cctacggcct gcaccgcgcg ttcggcaaga ttgagaacac cttggtagcg ggtcggagat
     3301 aggcgggcgg ggaagtgata ctcgctctgc cccttctggt acttcgactt gtagtcgtag
     3361 aatccgcctt tcggctcgat ttcgatggct ccgagtgcgt tgccatcgag caacccgaca
     3421 gcgacttcgc ggcctcggat caagcgctcc acgagaatcc actgatcgaa gcgcgcggca
     3481 tcctcagcgc gttgcctcag ctctgtgaga cttcttgccg cacccgctcc aacgctcgag
     3541 ccaccgctcc gaggttttac gaagctcggg aagccgaagg agctatggat ctgttcgagt
     3601 cgatccaagt catctgcccg caccacgtag taggatggtg tggggacatt gtagagacga
     3661 aaaagctcct tgctcttgag cttgtccatt gcgagcgcgc tcgaaagcac accggagccg
     3721 gtatacggaa tgcccatcgt ttcgagcatc ccttggatgc atccatcttc gccgtaggtg
     3781 ccgtgaaggg cgatgacagc cacatcgatc gagacctgac gaagcacccg gtcgatatcg
     3841 gcgtcgacga agaccttctg gaccttgtag ccacgagcct tgagggcctc gaacatagct
     3901 tcgccggtct tcagagagat atctcgctcc gcgctgacgc ccccaagcag tattccgatc
     3961 tctttgtcct tcattcgaat ctcctcgacg cgttacctat gcgaagagcg ggccaacccc
     4021 gcagcaccct aaattccgcg gccggcggtg ccgggctcgt gtctcgcgtg ccacagtcgc
     4081 gacgctctcc tgacacgaaa tgcccctgcc catgcctttt cggctgtaac agcggcgatc
     4141 ggtcagagcc aattaccggc aaggcggccg tatggtcctc ggcccgatca aagtcaaatc
     4201 catggggatt aaggcactag gcgggcgctc agctccgcga cgagggctgt ggaggtcgat
     4261 ttgtgacctt ttgaggcgtc gtttcgttgc ttcggcccaa gccggatggt accgaggaga
     4321 gacgaaaggg tcgttcgacc cactaaccca tattcaacaa ccgcttttgt ccggtcggag
     4381 tgcgagcgag cgtggctgat agccctcagg gcagcacggc aatgcgcggg tggagcgaat
     4441 caacgctccc cgtcggaacc gctcatatcg cccaccggag tgacaggaac gaacacaaca
     4501 atgacattca cggaagcagc cgtggaggtt ctccgatcgg tcggaaagcc cctccactac
     4561 aagaaaatca ccgaaatcgc gatcgagcgt aatctcctgt cgcacgtcgg taaaacccca
     4621 gaaaccacga tgagctcgag actcgccacc atggtcaaga aggaccgcgg cgatgctccg
     4681 atcctcaagg tgaagcccgg cgtattcgca ctgagggagt tccccgaaga tgtggtggaa
     4741 gacgcagaag gtgtgctcgc agacagcgat gcgcctgagg cgcttgaacg cgatggcgcg
     4801 gacgaagagc ccgcgtcgtt cgaggaaccc gcagagaccg aggaggagtc gagcctcgtg
     4861 gtgcaagatc tcccaggttc tgacgtgttc ccggaagaag atgacgacga cgagctgatc
     4921 ctcgccaatc tcgacgacga ctcgaaggat gcgcgaaagc ctcgcaagcg tcgcccacgc
     4981 cgtcggcggg acagggattc agacaacggt tcaagcgctg agtcacgcga ctcgcgggac
     5041 tcacgagact cgcgggactc acgagactcg cgagaagcgc gggactcgcg agaatcccgt
     5101 ccagatcgac gtccaccgaa tcttcgcggc cgaccgcagc gcgagccagt tgcgccggcg
     5161 acgcgcgcgg ccgagccggg tgaacccgtc ggcagggacc tttcggatgc catcgaaacg
     5221 gccctgcgtg gaggtagccg gcaggcacgg accctggttc aggtggccga agcgttgatc
     5281 gataccggcc gtctggccgg agcgcctcag ctcctggccg cgaccctcgg tgcagccatt
     5341 cggggcgaca acgccaagcg gcgcgcatcg ggcggccgac cgaggttccg ggagctcagc
     5401 ggactcgttt cgctactcga gtgggacctc cccggtgacg tcgtcaacgc cgagcgcgac
     5461 gccgtccgtg cggctgaccg tcagcggcaa agcgtccgcc gcgcgttcaa caagcgattg
     5521 cgggacctgc ccaatggcgc cttgttggag ctgctcgcca cctggctcaa cgccgtcggc
     5581 gtacactcgc ttcatgccgt ccgcgctaat gcaggcgact tcaaccttgc cgggacgctt
     5641 cgccgcggcc ctgaggaaac cccgctcgcg atcagcatct atcgtggaaa tcagccgatc
     5701 ggcaacgaag ccatcgtggc gctccgcggt gggctgcatc acttcgacca tgcgcgaatc
     5761 ggctgggtca tcaccctagg acaagcgcgc gatggaacac ttggagaagc gcacgccgaa
     5821 ggggcggcgc cgtgcgccgt gttcgatgga gatgcattgg ccatctcgat ggaggaggtc
     5881 ggcgtcggca ttcagcgcgc ttcacttccg cttgcggtcc tcgacctcga gcttctcgat
     5941 tcacttgccg gaggaagcgc gcgcagctcg gcgccgagga gcgcgcccaa caacgaggag
     6001 agcgtcgact cagagggcaa caagcgtcgg cgaggccggc ggcgcggccg gcggcgcggg
     6061 ggtcgagacg agaacggcga agaggcgcgc cgcgacgatt tcgctgaggc cagcgaaacc
     6121 aaagagagct ccgatgcgct cgacagcgag cgacccgagg ttgaagccgt ttcggattcc
     6181 agcccggaac ccgctgaaga taaagataaa aagggcgaag agccgcagtt ggcgccggag
     6241 ctcgaagcct gaggactgtg gtcaaataga cacacttgcg ctgcctgcgg gtggcgcaag
     6301 gtgagcgcat gccctaccgt cggggcgcct tgatggttat caggactacg gcatgcgaat
     6361 cgtactattg gtcataagcg ttgtgtttct cagtgcctgg cactcgcgta gtgaagcggg
     6421 tcccgcgttc attgaagttg cgcgtgcatc ggctaacgac atgatcgact accgcaaggc
     6481 cgtcggcgat catccgacct cgctttcgct cgcgcgcgcc atcgacgcac ggctcacggt
     6541 tcgcgaaagc cgcgaaggcg atcccattca cgtacagcac tgccgagaga gtcttggcgg
     6601 atgccgcgcg cgtatcgcga cgctgtcgcg cctgatcacc gaggcgagca accgagcagg
     6661 cgtcgacccc ttcctcaccg ccgccatcgc cgtgcgcgag tccggcctga acccattggc
     6721 ggtgagcccg gtcggcgcac gtggcgtcgt acagttgaac ccgaagtacc gaggcaagac
     6781 cgtgccgttc gtctacaacg agcgatacag ggagaaatgc agccttcgcc ccggtgcttg
     6841 ccagcgcgag gtgctcgagg ccgggctcgg cctgtatgga tgggcggtct cccgctgcgg
     6901 gggagatgtc gcgaagggcc tcgcctggta caattcagga cactgcgaca agcagaatgg
     6961 atacgcggcc tcggttctgc gggagcggcg ccgtcttctt cggctggcaa aagacaccga
     7021 tccgcgtcta gaagccgtct ttccgaacta aatccctgat ttctgggaca cgatgagccg
     7081 ctccaaggcc tcgcccatgg cctcggcgac tcgttctacg ggagcggcat cgggggcggg
     7141 atcgttggca cagaggacac ccaccatgcg agtcccaacc agaacgggca cgacgaccac
     7201 atcccgccca cgactgccga gagctgcccg aaagaggtga tcggcagcgg tctgtccgta
     7261 ggcgccccga aagggacgcc cagtgtgcaa gacctcattg agcaccgaag gattgctcgc
     7321 cggaacccaa aggcttcgaa tgctcgcgct ggtaacctca tcgccggccc cgtcccaacc
     7381 ccggaacaca cctttcctga gagcgaggaa cgctgcgccc tgagcggccg tcaagcaggc
     7441 ctcacacgcg agccgcgcca cctcatcgcg cgaggtcgcc tgcgtgagct catgcagacg
     7501 caattcgatg ttcgattcgg acgctggcgc aggaccaggc gtcgccggcc gctgcggcga
     7561 atgagagatc gaagggagtg ggacctctcg ctcggtagtg gagtggtgcc aggcgcgatc
     7621 ccatacggga ctggcagtcg aagcgagccc ggagaacgca ggatcgaact cggacagcgt
     7681 gggttgactg ccgacctcgg acttctcgtg agtgagcgcg aaggcatcgc tcggaattcg
     7741 cgagcgagcc agcgctagag gatcgctgac caaagtcgga cggtccggag ggtacgcccg
     7801 gtcgatcgaa gcgagaagat ccgagagctt tgcaaccgtc ggcaagatgc tcccgccaag
     7861 cgcttcaccg agccgcgcga ccgcatgcgc atcggtggga tcggccatcg cgacgattgc
     7921 gccggccggg ctcgggcgca gcggcatcgc gcaaaggtcg tgcgcgtcgg aggcaggcaa
     7981 gcggcgaacg agctcctgat cggcgcggcg aagatcctgc tcttgcagca tcgggccgaa
     8041 gccccgcgag atgaagaagc ctgccaagac gcgttcgtcg agtccggcgg tcaccagatt
     8101 tcgcaagacg cgtccagacg gcaccagcgg gtcgccagcg tggcttgcac tcgcctgagc
     8161 cgccgtgacg agtccggcgc tcacgagttg ttcgaccaat ccgactccca tggcggatcc
     8221 ctagcgaggg tctctgcgtg cggcaagccc gacgggctga tagcatccgg ctcgaatggc
     8281 tcgggtgctg cacatcgagg acgatcccaa caatcggcgc ctcgtgcaga agctgctcgg
     8341 agcggccggt cacgaggtca tcgaggccga gggcggggtc caaggaatcc agctcgcgcg
     8401 cgagatcctc cccgatttgg tcctggtgga catcaatatc ccggatctcg atggttacga
     8461 ggtcacgctg cggcttcgcg gaacgccggc gctccaggac gtgccgatcg tagcgatcac
     8521 cgccgaaggc gaccgagact cgactcttgc cgtgggttgc gatggattca ttgccaagcc
     8581 gatcgacgcg gcgcacttcg ccgaaacaat cgctgagttc ctcggagggc accgcgagtc
     8641 ggcggatgac gggagcgacc ggcttctaag agcgcgcacc cagaagatcg tcgagcggct
     8701 cgagaagaag atcgtcgagc tctcggaaac caatcggcgg ctcgaagaca tcgcgcgctt
     8761 gcgccgcgag ttcctgcaga acgtgagtca cgagcttgcg actccgatga cgccggtcgt
     8821 cggctacctg cgcctgctga tcaacgaaga gctcggaccg ctcagcgact tgcaacgaaa
     8881 atgcctgagc tcgatcgaat catccaccca gcgccttcga acggtcgtcg atacattgct
     8941 cgacgtgagc tctctcgaaa ccggacgcat gcactactac acgcgtccct acgacttccg
     9001 tgaagtcgcc atgaaggcgc tcgatcagat ccgcccgaag ctcgtcgaac gcgacgtcac
     9061 gctgaccgag cggattcccg gcgagccgat gcccgcgcaa ggggacccgg agaagctctt
     9121 gcgagccatg gttcacgtgc tcgacaacgc gtcgaagttc acgccgaccg gtggtgaggt
     9181 cgccgtggag gtccgccccg aaggggagga tcaccttctt tttgcggcag cagacagcgg
     9241 ccccggtgtc cggccggagc acatcgcccg catcatggag cccttctatc aagtcgacgg
     9301 atcggtgact cgagatcacg ggggcgtcgg cctcggcctg gctttcgcgc ggcgcgtaac
     9361 cgaagcgctc ggcggcggca tcgagatctc cagcccgccc gcgggcgaag ttgcgaagcg
     9421 acagctttcc gggacgctcg tcgagctccg cgtgggccgc atccccgaac ggcctgagat
     9481 tccttcgcag tgatttatct agaccatcac gcggcggcgc ccacacccga ggccgtccgg
     9541 cgcgccatgg acgctgccgc acccgcggcg tgggcgaatc catcgagcac gcacgccgcg
     9601 ggtcgcgccg cgcgccggct cctcgaagcc gcacgcgaag atgttgcgaa agccgtcggc
     9661 gctgcgcccg ctgacctcgt gctcaccagc ggagggacgg cggcatgcaa tctagggatc
     9721 cggggcatcg ccgcgggctg caaacgggtg ctcagcaccg aggtcgagca ccccgctgtg
     9781 gccgaatcgg ttcggaggct cggcgacgag gggcgcgaac gaatcgtgat cgccgtgcgc
     9841 gaggggcagc ccccgagcgg caaggagctc gcccgccatc tcggaccggg aaccctcgtc
     9901 gcggtccaat gggtgaatca tgagaccgga accgtcttcc cagttcatga atacgcagag
     9961 gtttgccgtg agcggcaagc gctcctgttc gtcgacgcaa gccaggccct ggggaagctg
    10021 cccatcgatc tcggtacgct tggagcggac gcggtcgcgc ttggcgctca gaagatcgga
    10081 ggaccgccgg gtgcaggcgc ctgctggata cggcgcggct tggacgtgct tccggtcctc
    10141 gtgggaggct cacaggaacg cgggcgatgg ccgggaactc cagacacact cagcatggtg
    10201 ggttttgggg ctgcatgtcg tctcacgggg aagcgcttgg aggaccaatc gcgcgtgacg
    10261 gctctgcgcg accgactcga agcctcgctc ctcgggctag gcggcgtcct gaatgggggc
    10321 tgcccaagga ccggaacggt ctccaacatc agcttcgcgg gctgggaagg gccgctgctg
    10381 gtcgctgcac tcgacctcga gggtatctgt gtgtcgaccg gagcggcctg ctcatctggc
    10441 atgcaggagc catccgccat aattcgctct atgtatccgg atgagcagtg gcgcgctggc
    10501 tccgcggttc gaatcagtct aggtatcgag acgagcgagc aagatatcga gacggcattg
    10561 cttgtgtttc ggcgcgtgct tggccgaaag ctcgcctaga acctcgagca aattttaact
    10621 cttcggagcc tcactttaga tgatttctcg ttttatgttc caagtaactg aaaatagaga
    10681 gctttctaga ggctattttt cgaggagcct ggtttccatt aagtgtaaca aaccacggtt
    10741 gatttgttca gtatggaact tcgatgggtg aagtgaagcg agtgctagtg gcgatgagcg
    10801 gcggggtgga ctcctcggtg gccgcggcgc tcttgcgtga aagcggacac gagctggtcg
    10861 gcgtaaccct tcatctctgg gatgcggacg gcgaggctca ggtcggtcga tgctgtgctc
    10921 ccgaggatcg agacgacgcg cgtcgcacct gcgagcacct cggcattccc cactacgtcc
    10981 tcgatgagcg cgcggcgttt cgcaagcatg tggtggaccc attcatcgcc gaataccagg
    11041 cgggacgcac gccgatcccc tgcgtgagct gcaatcgtac tgtgaagctc agctatttgg
    11101 ccgaggttgc tcgccgcttc gacgcgaccc atatcgcgac cgggcactac gcccgtctcg
    11161 aacgcggagg cgacgggcag cttcgactcc ttcgcggccg cgacggcgac aaggaccaga
    11221 gctacttcct ctttggcttg gcccaatcca ccttggaacg cctgatcttt ccgcttggcg
    11281 acctgagcaa atcggaggtc cgggaagcag ggaggcgttt gggcgttccg aatgcagaca
    11341 aagccgagtc gcaggagctc tgcttcgtcc cggacggccg cgtgggcgac ttcatcaatc
    11401 ggcaggggaa cccatccccg cctggtgcaa tcgtcgactc cgagggcgga ttgctcggag
    11461 ggcaccaggg cgtagccaac ttcacgcgcg gacagcggcg cggccttggc ctcgggggcg
    11521 gccgtgcccg gtatgttctt cggatcgtgc ccgatacagc ggcggtcgtc gtgggcgacg
    11581 agtcggagct ctacgacgac gcactcagcg cgacggatgc acactgggtc gcgggacgcc
    11641 ccgccgagcc ttttcaaggt gaggttcgca ttcgctaccg gcaccgaggc gcccgtgccg
    11701 acgtcatacc gaccagtgac gggttcgaag tgcatttcga cgagcctcag cgcgcgatta
    11761 cgccgggcca ggccgcagtg gtctaccgtg gagacgaggt gatcggtggc ggcttcatcg
    11821 cgtagagtcg gcacgcgggc ttttggattc gatgcccgct aagcacttgg cgttgggcga
    11881 agttcgcgcg gtggggcgcg gaggcgatgc cgtcgtggag accgctgacg gaatcgtctt
    11941 ggttccaggc gcattgccaa cggaacgcgt ggagatcgcg ctgggctcct cgaagcgcgg
    12001 cgccgcgcgg ggtattctga cgcgcatcct gcgagctgct ccagggcggc gcgagcccgc
    12061 ctgcgcggac gctctgaggt gcggcggctg cccgctcatg atcgccaacg cagaggttca
    12121 gcggcagatc aaactcggat tcctgcgtga cgcctgccgc gggctgcccg gttccgatac
    12181 gacggagatc gactggatca catcgccggc tgagctcgga taccgccgcc gcgccagact
    12241 tgcatggcac ggtcgcaaca tcggctatcg tcaacttcac tctaagcgaa tcaacgacat
    12301 tgccgaatgc atcgtcttgg cgaagccttt gcgtgaagct tggaccgagc tgcgcgcatg
    12361 tcttgcgcca tcactccaag gggagggcga gattcatctt caactgactg acaccgatcg
    12421 tgtgttggtc gatctgcgag ctgagcacga ccagctaccc aacctgtttg aagcatgcga
    12481 agcgctcagc cattcgccag aaatcgcggg cgtctcgctc cgaaccggcg agaccggaca
    12541 tgccgcgagt tggggagaga cccaggtccg gcacctacga gcagcgggcg ggtccttgga
    12601 aggtccagct ggcgcgttct cacaagcgaa cgacgccgtg aatgcgaagc tcgtcgaagc
    12661 cgtcatcgag ctcgccgagc ccgaaggtcg gcgtgttctc gagctccact ctggaattgg
    12721 aaatttcacc atcggactcg cggagctcgc caagactttg gtggcggtag agcaagaccc
    12781 gcaggctgtc caagcctgcc aatccaactt ggagcgccgc ggtctccaag cgcgcgttac
    12841 cgtcggcgac gcgaaccaac ctcccaaggg aaagtacgag gtcatcgttc tcgacccccc
    12901 tcgccaaggc gccaaggccc ttttcgagcg tagcgacctg ctgccagggc ccaagcgaat
    12961 cgtctatgtg tcttgcgaca ccgccaccct cgcgcgagat ctccgcattg ccactgcgaa
    13021 aggttatcgc atcgagcggg ccatcggctt cgacatgttt ccgcagaccg ctcacctcga
    13081 gtcactcgtt cttctcgtcc acgcttgagc ggcgccgctg cgttgcccgc tctccctgcc
    13141 ggagctagct tgtgaccgga tatgcgctgc ccctactgtg gaacgctcga aaaccgggtg
    13201 atcgactcgc gcctctcgca ggctggagag gtcacgcggc gtcggcgcga gtgtgagcat
    13261 tgcgggcgtc gctacaccac ctacgagcga gtggagcaaa cacttccgct catcatcaag
    13321 acggacggtc gtcggcaacc ctatgatcgc gacaagctgc tggccggcct gcggcgggcc
    13381 tgcgtaaaac gaccggtctc tgccgaagcc ctcgagcgct tcttgcatca ggtggagcgc
    13441 tggttggtgg acaccggcga gcaggaagtc acctccgcgc gtctcggaga gcacgtcttg
    13501 cacgagctgc gcggcctgga tccggttgcg tacgtgcgct tcgcgagcgt ctatcgagat
    13561 ttccagaacg tcggcgagtt cctcgacgag ctcgccaagc tcaaggattg agatgtggtc
    13621 ggacttcgag cgggaggtga tggcccgagc gattgcagaa gcccggcgcg ggcggacgag
    13681 cccaaatccg cgcgtcggcg ccgcaatcgt cagaaacggg aagctggtct cgctgggcca
    13741 tcacgcgaag gcgggcgagg cacacgccga ggtcgatgcg attcgaaacg cccggggatc
    13801 cgtggagggc gcggccttgt acgtgacctt ggagccttgc aaccaccacg ggagaaccgg
    13861 accttgcacc gaggcgattt tgaaggcggg gatcgcgaaa gtcgtcgtgg gctgccgtga
    13921 cccggcgccg cacgtggcgg gagcgctcga tcgtttgagg agcgccggtt tggaagtcga
    13981 gctcggcttg atggagcgcg agtgcacggc gctgatcgcg gatttcgcga agcacatccg
    14041 aaccggtctg ccgttcgtga cgctgaaggc ggcggtgacc ttggatggaa agatcgccac
    14101 ccgaagcggc gactcgaagt ggattacagg cgacgaggcg agggccgaag ctcaccgcat
    14161 gcgcgatgag agcgacgcga tactggtcgg cgtgggcacg gtgctagccg atgatccaaa
    14221 gctcaccgtc cgtcacgtcg aaggacgaaa ccccgttcgc gtagtgctgg atgccaacct
    14281 tcgcacgccg aaaaccgctg cggcgctgaa gcaagacaaa gcctcgaagg ccgaggtttt
    14341 gatattccac gctcacgatg ccaacgtcga gcggcgcgaa tctctgcgag gaccgggcat
    14401 cgagctgatt cctgtggctc gtcacgcgcg tggtgtggac ctggtcgagg tcttgaagct
    14461 actgggtgaa cgcggtttcg tgcggctctt ggtggagggc ggcgcgcacg tgcatggcac
    14521 gtttctggat ctccacctcg ccgaccgcgc tgccatcttc atcgcacccc ggatcgtagg
    14581 cgatgccaat gcgctttcat tcgcgctagg ggacggagtg gacacgatgg agcaagcgtc
    14641 ccgtttggtt cgaaccgaga tccagacttt cggaaccgac tggttgattt cgggggactt
    14701 cgagaggacg cattgaatgt ttaccggact gatagaacag ctaggcacga ttgaagatct
    14761 cgaccagcgc gaagacgggc tcagcattcg catcggctgc gcacttcacc cgtttgagct
    14821 cggggaatcg atcgccgtca atggaacgtg cctcacggtg aaatcgtttt ccgagcggca
    14881 cttcgatgcc gacgcgtcca acgagacgct ggacaagaca aacctcgggc atctgagcgt
    14941 cggcgacaaa gtgcatctcg aacgcgcgct agctctcggc gagcgcttgg gtgggcatct
    15001 ggtcactggc catgtcgatg gggtggggac gctcagcggc aagactccgg acggtgacta
    15061 cctccgcgca agctttgaag tccccacacg gctggctccg ttcctcgccc cgaaggggtc
    15121 gattaccgtc gatggcgtga gcctcacggt gaacaccgtc tcgggctcgc gcttcgatgt
    15181 catgctcgtg ccatacaccc tcgacgagac cacttttggg gagatgccca tgggcgccaa
    15241 ggtcaacctc gaggtcgaca tcctggccaa gtacgtcgca agcctgatgg ggaagcccgg
    15301 cgtagacgcc atcgaagact gaggatttgc agtgttccgc tggtatgagg gacgctttcg
    15361 cgtcatggtg gcggccatat gacgagcgcg atcgagcggg tcgtcgcggc actcgatgac
    15421 atccgcaagg gtaagatggt catcctggtc gacgacgaag accgcgaaaa cgagggcgac
    15481 gtgtgcatgg cggccgaaat ggcaagccct gaggcgatca atttcatggc aaggcatgcg
    15541 cgcgggctga tatgcctcag cctcaccgaa gaccggatcc gtcagctcga tcttccgatg
    15601 atggtcgacg acaaccgctc gtcccgctcc accgcgttca ccatttcgat cgaggcacgc
    15661 agaggggtca ccaccggcat cagcgcgggt gatcgagccc ataccatcct caccgcggtc
    15721 gcagacgacg ccggcgccat ggacttggtg agcccagggc acgtctttcc gctgaaggcc
    15781 gtgcccggag gggtgctcca gcgaacgggt cacaccgagg gatcggtcga cctctcacgg
    15841 ctcgcaggct tgaagccagc gagcgtgatt tgcgagatca tgaaggacga tgggtcgatg
    15901 gcgcggtacc cggacctggt cgccttcgcc gaagcccacc agttgcggct cctcaccatc
    15961 gcggacctca ttcagtaccg gctcgcgcgc gagcgcctgg tcacgaggat ccggcagacg
    16021 cccgtcaaaa tgcccaccgg gcgcacctgg accgcgtcgg tctatcaggt ccaagttggc
    16081 ggccaggcgc agttcatggc actgagcctc ggggagatga gcgcggaacc gacgctcgtt
    16141 cgtgtccacc gcggcagcgt gcttggcgat gcctttcagg tccgcatggg tgcgcgggtg
    16201 cacatcgcag actgcgtcgc ggcgatcgaa cggaagggca acggcgcaat cctgttcttg
    16261 ccgggtcacc ccgaccccga aaccgatctc gcattttatc tcggcgagcc catcccgcgt
    16321 ccgcccgaag agcccggcgt ggtgcttcgc gaatacggat ttggagcgca ggtactctcc
    16381 gatctgggtc tcgagcaact ccttctgctg acgaaccggc cgcggcggat cccgagcctc
    16441 gacgcttacg gcctcacggt cgtcgatcag ctcatggtgg cgccaggcgg ggagctgagc
    16501 tcggcgtccg agcaaacgcg agacgaaacg tgagcgacga tcgcaaccaa cctctcaccg
    16561 aggtcgaggc tcccgcaaat gctcgctttg cgatcgcctg gtctcgcttc aatcaccacg
    16621 tcgtcagtag gcttctcgac ggcgcaaagg cgtgtttttc ggagcacggc ctttcggagt
    16681 cctcgatcta cgtcgtcgaa gtgccgggtg cttgggagct cccccatgcc gcgcagaaac
    16741 tcgccgaaag cggctgcttc gacgcagtgc tcgtcctcgg cgcgatcatt cgcgggggca
    16801 cgcctcattt cgattatgtc tctcaaggca ccgccctcgg cattgggcgc gtcgccttgg
    16861 acacaggtat tcccgtggtc tttggtgtat tgacgacgga tgacgagact caggcgttgg
    16921 agcgcgcggg cggcgcacac ggcaacaaag gccgggacgc agcactgacc gcgatcgaga
    16981 tggcggcatt cgctcggaag cttgtgaacg atgggatcaa gacgaaaagg gcgtgaagcc
    17041 gcgctgcgca tcctgtactt catggatgtg tcgggggtga gcggaacgca ggcgatcaaa
    17101 tcgtactggg ggcatctcgc ttcggaagag gagcggaccg aggatgtcga cttcgccaac
    17161 cggctcgttc gcgcgtatgc cgaccgacag gatgaggtga acgatctcat tcgctcctcg
    17221 agtcatcact ggcgcctcga acggatgcca atcgtcgacc gcaacgtcct gcgcgtcgcc
    17281 atcgtcgagc ttcgtgaatt gaacgacatt cccaagcgcg tcacactcaa cgaagccgtg
    17341 gagctcgcca agcgatttgg atccgaggga agcggctcgt tcgtaaacgg tgtgctcgat
    17401 cgaatcgcca ccgagctcgg aaaaccgtga tcgcctacga cttcacgccg gcttcggcgc
    17461 gaggcctcga tgagctgcaa acccaattgc tcgtgctccc gttcttctcc gatgaacgcc
    17521 ctctccgggg ccccgcaggg ctcgtcgatt ggcgcctttg tggagcgcta tcccgcaagc
    17581 tgatgtcggg gtacatcaaa ggaagctttg gcgagaaggc cctcctgacg acgcctgcga
    17641 agctccaagc ggacggtcta ttgctttttg gccttggcga gagcagcgac ttcagcactc
    17701 ggatcgccga gaaggcctgt ctgttgatcg gagatgcttt gactgaggcc aaagtgtcga
    17761 ccgcggcgct tgccttgccg gggcggagca tgcgtcttct cagtgcgctc gaggcgatgc
    17821 agatctggct cgattgtagc gataccgctc gggagctcga agagctgagc gtcatcgagg
    17881 agggaccggc acaccgctcg ctcgactcac tgctcgacgg cctgcggcgc caagcagagt
    17941 caccactcga ctagcggcgc gaggaccggc gcttggcttt cttggccgac gtcttgctgc
    18001 gtttcttcgt tggtctgcgc ttggctggca cggctcttcg tttggccgcc gtgcttcgtt
    18061 ttttcggcgc gctcttcttc ttggctgcgc tgcgcttttt ggtgacgctt cgcttcttcg
    18121 gcgcgacctt cttcttggcc gcactgcgct tttgggtgcc gcttcgcttc ttcggcgcga
    18181 ccttcttctt ggcggaggtc cgcttcttct tcgcgccgcg cttctgctgc gtcgccttgc
    18241 gcttggtcgg cgtcccacgg cccttggcgc tcgtcttctt cttggcagct gccttcttct
    18301 tggcagctgc cttcttcttg gcagccgcct tcttcttggc agccgccttc ttcttggcag
    18361 ccgccttctt cttggcggcc gcgttcttcc gcttgatctc ctcggccttc ttcttcgcgg
    18421 ctgctttctt acgcttgagc tcgtcggcga ccttcttggc ggccgccttc ttctgcttga
    18481 tctcctcggc cttcttcttg gcttcggcct tcttctgggc agccgccgcc ttgcgttcga
    18541 gctcggcttt cttcttttcg gcagccttac gcttcgcctc ggccttcttg attctcatca
    18601 gctcggcctt cttcttggct tcggccttct tcacagccgc ttcggcctgc cgccgagctg
    18661 cctcggcctt ctgcttggtt tcagccttcc tccgagcggc ttcctttcgg gcctctgcct
    18721 gggcgagtgc aagctctcga agatcgatcg tcttcgattc gaaacgcgca cgcgtcttct
    18781 ttggcttttc ctcttcctct tcgtcttcgg aatcctcaat gggaggcggc ggagccttga
    18841 tgaagggccg ccgaggtgcg ggccggctcg ccttgatccc agaaagcggt aaatggccgg
    18901 ctttcttggc ggcggtgtag gccgctcgca tcgcgtccgg ctccccgaag tacctggcca
    18961 tgggcttcag atagagtttg ttccagatct cctcgcaggc tttcccgagg ccggcgggga
    19021 tcttggagtg ggttacctcc accttcgtcc caccggccac gggctcgaag gtgatatcca
    19081 cctgagagtc attcgagccc ttggggaaat cgcgagtccg ccaggtcatg gcgatacgac
    19141 tgccggtatc gagatagacg tgcgtcgcgt cgatgaaacc atcgtccgta acgactcgac
    19201 cgcctaccca ggggtccacc gtggcccgag cgcccgtaaa cgcggagtga tggcgctcgt
    19261 ccatccatgc ggaataaata cggttagggc tcgcgggtat cacggacgag acagtgaatt
    19321 cttccgtgtc catcttcctt agcttccttg acttgctggc ctgtataagt ccaggaaact
    19381 catttgaaaa ccagaaaatt gcggcgggtc ggattccgaa gggtgcattt atgacctcct
    19441 tgtaagctat ctgggcgatt tctgcaccca aacagtgaga tcgcgctgga actcttgacc
    19501 cgctctgggg ccgtgcttcg atgcaggcct acatggaaga gccgaccccc cggcaccagt
    19561 tctggatcga ccgcggcggc accttcaccg actgtatcca tcgcgatctg gagagcggcg
    19621 cgttgcgggt cacgaaggtt ctctcctcgg atcaggctcc cctcgaaggc atccgagcat
    19681 tactgggtct cgggccaagc gattcgatcc cggcgagcga ggttcgcatg ggaacgacgc
    19741 tggccaccaa tgcgctactc gagcgcggcg gccggccgac tgttctgatt gcggatcaag
    19801 ggctgggtga cctcattcgt atcggcgacc agacccggcc cgatctgttt gctctcgaga
    19861 tcgaaaaacc cgagccgcta cccgcttggg tcatcgagat tggcgcacgg atgaatccag
    19921 acggttctat ttttcgcgag ctcgacgagt cggagctgcg aaacgcgctc gaggaagccc
    19981 gaaatagcgg cgccgaatcg gtagcgatcg ctttgatgca cgcgtaccgt gacggaacgc
    20041 tcgaaggccg ggtcggcgcg atcgcgaaag aggtcggatt cgcccatgtc tcgacgtcga
    20101 acgagatatc caacgagatc ggcctccttg gccgcgccga tacgagcacc gcaaacgcat
    20161 acctcacgcc attgctcact cagtacatgg ccgcgctcga aaaggagctc ggatccagcc
    20221 gcttgcggat gatgcagagc aacggaggct tggtcgacgc atccggcttc atcggtcgca
    20281 acgcggtcct ctcaggccct gcggctggag tggtggcgac cggcgcaatc gccgaagagc
    20341 tcgggctctc ggaggtcatt ggcttcgaca tgggaggcac ttcaaccgac gtgtcccggt
    20401 tcgcaggaca ggtttcgcgg gtattcgaga gccaaatcgg cggcatccga attcgcgctc
    20461 caatgatgga gctccacacc gtcgcatctg gaggaggctc gatctgcaag ctcgaaggcg
    20521 agcgcatgat cgtcgggccg caaagtgcgg gttcggaccc gggaccgctc tgctacggca
    20581 actctcaggc cgaggagctc actctgactg atgtctcgct ttgcttaggc cgcttgagcg
    20641 ccgaccggtt cccgtttccg ctagacgtgg agagagcaaa gaagaggctt gctgcggtcg
    20701 ccgcatcgct tcgtgcaacg ggtgtcgaac gaagcgcagt cgaggcggct catggttttc
    20761 tgcgcgtggc cgtagagaac atggcgaccg ccatacagaa gatttctgtc ggacgcggcc
    20821 acgacgtgcg caatcacgcc atggtggtct tcggcggcgc tggcggacag tatgcttgcc
    20881 tgatcgcgcg tcgcctcggt atccgcgacc tggtctttca tccgtatgca ggggcgcttt
    20941 ccgcattcgg tatgggggtt gccaatttcg agtggcacgg agagcgtgac gcagggcgcg
    21001 ccgacttggc cgcacctttg ccgaccgggt gggaaagggc gttcgaggac ttggaggcct
    21061 caggccgcgc tgccttggct cgagatgctg gcgctcaggc gcgttggaac gttcgtagaa
    21121 gggtggcgct gcactaccgc ggaaccgaaa cgacgctcac ggtggattgg acggacgctc
    21181 atacgatgcg cgctgatttc gaagtcttgc accgccgtga gtttgggtac gttcggccgc
    21241 agcatgcggt ccacgcggcc acgctgcggg tccaggtcga attgagcagc cccaagccgg
    21301 atcttccgcg ggtcgaggct ggatccgggg cggcgctgcg cacgacgatg ctctggtccg
    21361 acggcgcact ggtcgaagct cccgtctacc tccgcgaaga tttcccgctc caaacagagg
    21421 tacgtggtcc cgcgatgatt ctggacgcca cgggcaccgt cgtcgtcgat tcgggcttcg
    21481 tcgccatccg agacgagcgc gactacctga caatgcgaga cgttgagagc cggatcgagc
    21541 gagaaatggc tgatactcga gtggatccgg tgctgctcga ggtcttcaac agccagtttc
    21601 aatcgatcgc ggagcagatg ggtgccgtgc ttcaacgcac ggcgatgagc acgaacatcc
    21661 gagatcgact cgacttctcg tgcgcggtgt tcgacgtgac gggggggctc gttgcgaacg
    21721 cgccccatat ccccgtgcat ctgggcgcca tgagcgagtc ggtgcgggcg gtcctgcatg
    21781 cgcatcccga catgaaaccc ggcgacgtct tcgtcaccaa cgaccccttc gccggtggct
    21841 ctcaccttcc cgacatcacc gtggtcactc cgcagcatga cgagaagggc gtgcttcgat
    21901 tcgtcgttgc gtcgcgcgga catcacgccg acgttggcgg tctgacgcca gggtcgatgc
    21961 ctcctgcgtc gacacggcta gaccaagaag gggtcgtcct acgcactctc cgaatcgtgg
    22021 aagacggagc gtttcgagag cagcttctcc gggaagctct cggccagggc aggtaccccg
    22081 cgcgcaaccc cgacgagaac gtggccgaca tccaagctga gattgcggcc aacgagaagg
    22141 gccttcagct gctcgacgat ctcttggcac gctacggcct cgaagtcgtg atcgcgtaca
    22201 tgcagcacat acaggacaac gcagccgcac tcacgagccg cgccatcgcc gatctgcccg
    22261 acggagagta tcgattcgag gatcaacttg acgatggagc ccgaatactg gtttgcgtca
    22321 ccgtcgatgg cggcgagatg tcgatcgatt tcgaagggac cgatagggcg ctcgacaaca
    22381 atctcaacgc gccaagggcc gtcaccatgg cggcggtgct ctacgtgctt cgcgtgctgg
    22441 tgggcaaacc cataccgctc aacggtgggt gtatgagacc ggtctccgtt cgcatcccag
    22501 agggctccct gctttgtcct gagcccggcc acgcggtagc ggccggaaac gtagaaacat
    22561 cgcagcgcgt ggtggatgtc ttgttcggag cgctcggcct tgctgcagct agccagggaa
    22621 cgatgaacaa catcaccttc ggcgacgatc gctttgcgta ctacgagacc ctcggagggg
    22681 gagcgggcgc cactgcgcgg cgaccgggcg cagatggcgt tcatacccac atgacgaact
    22741 ccaagtgcac cgacccggaa atcctcgaaa cacggtttcc ggttcggatt cggcggttct
    22801 cgctccgtca cggctccggg ggcagaggcc ggcttcgagg gggcgacggg ttgattcggg
    22861 agctggagtt cctcgcgcca atgcgagttt cggtgatctc cgagcggcgt ctcgtcgcac
    22921 cctatggtat gcgcggcggc ggttcaggtg caacgggcat gaatctactc aacggaaagc
    22981 aaatcgctca ccgcgtcacc gttcaggtgg cgcccggcga catcctccga gtcgaaacgc
    23041 ccggcggcgg cgcgtggggc gaatccgaat gaacctcaag gacctctggc gacgccttca
    23101 gcagattgtc gttcacaata tcctgcacct cgatgatacg ccgcatcgca tcgcctgggg
    23161 tgtattcatc ggcgcaatca tcgctttcac gccgacgctc gggttgcaga tcgtgatcta
    23221 cctgcctctt gctgccttgc ttcgcgcgaa caaggtgtcg ggaatcccga tcctgttcat
    23281 cagcaaccca tttaccgccg taccgctgta ctacgcgact tggtcggtcg gagcagccgt
    23341 gcttcgtccc gaaaaggaag tcacccgagc cacgatcaag acctggcttg gcaacatggg
    23401 gcgcgcgctg aaggatggcg gagtccgtcg attgctcgac acggagttct ggagcgacgc
    23461 gggacgcttg ctcgcgacca ccggcggtga gctttggatc ggtggactcg tctgcggctt
    23521 ggcggtggcg cttcctgcgt acttcctgac gcgctggggc atcaacgccg ttcgtcatct
    23581 gcgtgaaggc cggcacctgc cgcacggccc gatagaccgc aaatcgaacc cgaagctgaa
    23641 gagcgaaggt tagccggcag gccgctctca ccaggcccac cggctcggca tcagacgttc
    23701 ttcgcggagc cttcgacgcg cttcgctagc tcgcccatcg actccgcaag ggtgctgatc
    23761 acctcggcgg tggcggagag ggtcttggca gcagcctcga tcgcgctgcg cgtgacccca
    23821 agccaaagcg cggtgacctt acgtcccatg ctcagtgcgt cctcggctgt cttggtgact
    23881 tgaccgacgg ccgcattgac gttctcttcg acagcagatt gcatcttttg ttgttcagtc
    23941 atgggctttc tcctcgttgg atgtcggtgc ctggggcagg gcctccctgc ccgttattag
    24001 ttttcgacgc ggatcgtcga aaaggaccat gtaggcctcg gaaccggccg ccaatgttgc
    24061 gccagtcacg ccgcaaaccg cgtcatggac caaaatgatc ggcgtcacgc cgacggcgcc
    24121 ctcgagtgga tcggcctggc gttctgccgt tgcgccgtat gggccctcag gcggttccgt
    24181 tcgatcggat gctcccggga ttctgctcag ctgttcggcc gacttctttc gaaatcggtg
    24241 gcgttcagat gccagcaact cggctacttc gcggagctca ccttcggctt ttgcgcccac
    24301 gacgtcgagc gtgtccaacg cgtcctccag cagggcgcga acaacattcg acacgggaac
    24361 gcgccaagcg ttggcgaccc tcttgagctc ctgctcgaga acggccggca cgcgggtatg
    24421 gagcacccgt tccttcttgg cccgtcggct cttgctctcc tcgtcttggc ccatgcccat
    24481 catgtaacac tttgtgacac aaaggcaagg atctctgtgt cacacaggct ccaagcaccg
    24541 atttgaccca gcaaattcaa ctgttacagt cgccggccgt gactcgaaag caccgctcct
    24601 caaaggcatt tgttctctgc ctggtcgccg cggcgctcct cgcggccatg gacctcggca
    24661 gcaagcactg ggccctcgac cgactctctc agccctcggc gcaaggggcg gaaccggtct
    24721 gcgagcccaa cgagtacggg cgaaccgagc ctcagcgggc ccagcgctcg cccatcgtgc
    24781 tcatcgatgg atttctcgag tttcgatatg ccgagaactg cggtgccgcc ttcggattca
    24841 tgcgggacat gccgagcctg gttcggaagg gcgtctttta cgttgcggct acgggcgcga
    24901 tcgtcctgct tctctggatg ttcgtcaccg gtcgcggagg cgcgctcttt gcgatcagtg
    24961 ttccgctcat cgtggccggg gcgatcggaa atctcgtcga tcgggtccgc cttggctacg
    25021 tcgtcgactt cgtgcgattc cacatccacg atcgctgggc gtaccccacg ttcaacatcg
    25081 ccgacgcatg gatcaccatc ggagtcgcgc tgatcttgat cgaaggcttc gtcgatggac
    25141 ggcgcgaaaa gcaggagcgc gcccaaaaca ccgaaggctc ggccggggaa acctgaccct
    25201 tttccgattc aggccgcagt gatcgcgagg ctcagaggcg cgccttcgat ttcggtatcg
    25261 tgttcgctgt gctcgggcgt tgcggttcgc ccgaatgcgg tggccagggt ctccgtggcg
    25321 atccgggatg cgttttcttc gatggcagcc gcaagctcac cttcggcgtt ccaactgacc
    25381 tcgattcgag cttcgtaggc cagatccatg gccttgcggg cgcgttgaat gcgattgatc
    25441 acctcgcggg ccaggccttc gcgccgcagg ctttcgtcta cgcgagtgtc gatcacgacg
    25501 acctgacctg cacccgaggc cgcagcgaag tcttctcgcg cgctcaggcg aacttcgacc
    25561 tcgtcggggg tgagctcgat tgggccgtcc ggaagctcga tcacgatctt ggagttctgc
    25621 tccatttcga cgtaaagcgc ggagccatcc gcctccgaaa gcgctttctt gcaggccgga
    25681 acctgtttgc cgagctttgg cccgagcacc cggaagttcg gaacgagctc gaactcgaca
    25741 tacttgtgcg gttccagagt gaagccgagc tctcgcacgt tgagctcctc gcggatcgcg
    25801 ctcgagaagc ggtcgatcgc tagacgctca gcatcgtcgg ccacgaccgc gatggcctcg
    25861 gcgagcggct gacgaacttt gagcttgttt tcgtttcgca cacgctgacc cagcgtgacc
    25921 aaattgcgaa cccgccgcat ggtctgtcgc agttcgtcat cgacgcgcgc ttcttgtggt
    25981 tcggggaacg ccgccaaatg cacggactcg gcggcagtgg cgtccccttg gcgcaccaga
    26041 ttctggaaca gggtttcagt catgaatgga atgaacggtg cagcgagctt ggtcagatca
    26101 accagcacct cgtagagcgt cccgaaggcc gcacgcttgt cgaacgaatc ctcggacgcc
    26161 cagaatcgag cacggcttcg tcgcacgtac caattcgaaa cgccatcgac cagctcgagg
    26221 agatgccgaa ccgccatgtg gctcttgtac tcatcgagct cctggcgaac ggcacgaacg
    26281 gtcgcgtcga gctcagcgag cacccagcga tccatgtcag gccgttcggc aagcataggc
    26341 cgctcgtcct cgcggctcca accgtcgata ttggcgtatg tgacgaagaa gctgtagacg
    26401 ttccaaatct tcaggaggaa ttcgcgcacc gcgtcggtgg ccgcatcgtc ggaaaagcgc
    26461 gtattctggc cgggaacggt gcctgagtag agcgcccagc gcacggcatc ggcacccacg
    26521 cggtccatga gcaccagcgg gtccgtgtag ttcttgtcgc gcttgctgag cttcttcccc
    26581 ttctcgtccg tcatcagccc gagcacgacg caattcttga aagggtgagg aagtgcacgc
    26641 tcgggaaaca tgagcgagct gatcgtcatc aaggaataga accatcctcg cgtctgatcg
    26701 accgcttccg tgatgaagtc ggcgggaaag ctccgctcga agccttccga gttttggtgt
    26761 ggaaaccccc attgggcgaa cggcatgcag cctgaatcaa accagcagtc gatgacctcc
    26821 ggcacgcgtc gatacacgcc cggctcgcca tcgcgggccc aggtgacctc atcgacccag
    26881 ggcttgtgga ccatcaagtg gtcttgcaag ctggggtctg ccgccttggc tttttcgaaa
    26941 tgcgcgaagg cgtcgggatt gcgctgcaga atctcggcaa cggagccgat cgcttccacc
    27001 gcacccgtct cgtcgttcat ccaaatcgga agtggtgtgc cccaaaatct ctcgcgcgaa
    27061 agcgcccagt cgacgttccc ctcgaggaac tggcccatgc gaccgttctg gatgtgctcc
    27121 ggctcccagt gcacgcgttg attgttctct ttgacgcggc cgatttcttg ggtggtgcgg
    27181 atgaaccagg aacgtcgtgc atattggatc aacggatcct gcatcgcccg ccagcagaac
    27241 gggtagtcgt gccggtaggc ctcctctttg aagagcacat cacgggcgcg gaggttccga
    27301 atgatgtcgc gatctgcgtc tttgcagaat cggccggcaa aatcggtcac ttcctctgga
    27361 aacgtgccat cgggcttcaa gagctgaaga aaacccatgc ccttttcttt gcagatccgg
    27421 aaatcgtcct cgccgaacgc cggtgcgagg tgcaccaggc cggtgccact gtcgagcgaa
    27481 acgaaatcgg catcgacgac ctgccaagcg gggccgcctt cggggtcggc atatcggaac
    27541 ggcggctcgt aacgcattcc gaccagctcg gctcctttgc gcgtcgcgac gattttggca
    27601 tcgtctccga gcaccttgct gaccagggcc tcggcgagaa ccaatcgctc gccgtcgtcc
    27661 aactcgacgg tcgcgtagtc cgtcttggcg tcgaccgcga gcgcgatgtt ggatggaagc
    27721 gtccatggcg tggtcgtcca agccagaaac gaggtccgcg tttcgccgag cacggggaaa
    27781 cgaatcatca ccgatggatc atcgatggtt tgatagcctt ggccgacttc gccggccgag
    27841 agcgcggtgc ctccctgcgg ccaccaccaa acgactttgt aaccttggta gagtaggcct
    27901 tggttgaaca tctccgaaag cgcccaccaa acgctctcga catacgactt gtggaaggtc
    27961 acgtatgcgg aatcgaaatc gacccagaag ccgatacgct ccgtcatctt ttgccactcc
    28021 ccgatgtact tgaacaccga gtcgatacac ttgcggctga atgcttcgac cccgtacgct
    28081 tcgatcgcct ctcgcccgct gataccaagc tctttctcga cctcgacttc gacgggcaga
    28141 ccgtgcgtgt cccaaccagc gcggcgcggc acctcgtagc cgcacatcgt cctgtaacgc
    28201 agaaaaacgt ctttcatgac ccgcgtgagc acatgccccg ggtggggcat tccgttggcc
    28261 gtaggcggac cctcgtagaa aatgaacggc tgcttgtcct ttccgcccgc cgaggccttt
    28321 tcgaagatcc gggcgctttc ccaaaacgcg agttgggtcg catcgagctc tgggaaggaa
    28381 acctgcgggg agacggggtc gaacatggcc acgcggcata ccacgacatg ccgtgatttc
    28441 caccggtcct gctggtttga gaactggcga ggctcctgat agaccctcgc ccatgactga
    28501 actgatcgat gggaaggcgc tcgccaaaga ggtgagggcg gaggtcaagg cgagggcgga
    28561 ggccttcaaa gcgacgcaca accgggcgcc gggtttggac gtggtgctcg ttggggaaga
    28621 cccggcctcc gaggtgtacg tccgcaacaa ggagcgggcc gccgccaagg ccggaatcca
    28681 gggacgcgtc catcgactcc cagcgcagac ctcggaggcc gatctcctcg cgctgatctt
    28741 cgagctcaac ggcgacgatg agatcgacgg catcctcgtt cagctgccgc tccccgatca
    28801 tctgcacgat caaacagtca tcgatgcgat cgatccagcg aaagacgtgg atggcctgca
    28861 tccgttcaac gcgggtcttc tcgtggtggg tcgcaagggc ctccgcccct gcacaccgag
    28921 cggctgcatg cgcatgctcg aacgcgtcgg ctgcgatccc aacggcaagc gcgctctagt
    28981 cctcgggcga agcactttgg tcggcaagcc gatcgcactc atgcttctcg agaagcacgc
    29041 caccgtaacg atcgcgcact cgcgcaccaa ggatctcgca gaccgcgtgc gcgagtcgga
    29101 catcgtcgtt gccgcggtcg gccgacccaa cctggtccaa ggtgattgga tcaaggaggg
    29161 tgccgtcgtg atcgacgttg gcatcaatcg actggaggac ggcacgctca cgggcgacgt
    29221 cgagttcgag ggcgctaagc agcgcgcgtc ctacatcacg ccagtcccgg gcggagtcgg
    29281 ccccatgacc atcgccatgc tcttgagcaa caccgtcgac gcggccgaag cccgcatcct
    29341 ctgaggagcc cctgacgacc acactggcgc tggcaccggt actggcgctg acactgacgc
    29401 tgacgctgac gctgacactg gcgctgacac tggcactggc actggcactg gcaccttgac
    29461 gatcggaggg cggctcgcga cgcacgtgct cgcgattggg acccgctcgg ccgtctctcc
    29521 aagattccct gcgtctcgcc tcccgcccaa tcgctgtgcc gcgcatcccg agccgccctc
    29581 cgctgtacgc ggcgacgcac gcgcactgac accggcactg ggggtgtacg gagg
//
LOCUS       DASRZF010000015         6230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3793556, whole genome shotgun sequence.
ACCESSION   DASRZF010000015 DASRZF010000000
VERSION     DASRZF010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 6230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6230
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3793556"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1446
                     /locus_tag="VFG22_00835"
     CDS             <1..1446
                     /locus_tag="VFG22_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HET6414808.1"
                     /translation="FPIDDLRILLGKTIIPVAAAEETIDDAINRVYERKDETALAEAK
                     EGEEVEELRDLIDMTDEAPVIRWVNNLFYTAVKERASDIHIEPTDEKVIVRYRVDGRL
                     IPRKEANRGFLSSIVSRIKIEAGLNIAEKRLPQDGRITKKIAGKVVDVRVSTIPTAKG
                     ERVVMRLLDKEQVLLNLIDLGFARRELDQLEHLIGRPNGIILVTGPTGSGKTTTLYGC
                     LNKINTPEKNILTAEDPVEYELRGIGQMQVQPKIGLTFAAGLRSFLRQDPDVIMVGEI
                     RDHETAEIAIHASLTGHLVLSTLHTNDAPSAITRLVDMDIQAYQISSSVLAVLAQRLV
                     RKLCDHCKTPYAPTPQDLRELGIDADSAKEKLAELRRVAATTVIGHGTPSTLPNPANA
                     SSDELTFFHHHGCDACSNTGYRGRIGIFELMIIDEPVRREILNNSDAKTIQRVAQHQG
                     MRLLREDGARQVVAGVTSVEEVLAATQAAEV"
     gene            1450..2673
                     /gene="gspF"
                     /locus_tag="VFG22_00840"
     CDS             1450..2673
                     /gene="gspF"
                     /locus_tag="VFG22_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HET6414809.1"
                     /translation="MAVYAYKGVNARGKSVKGIRDADSAKALRTVLKRDGVLATEILE
                     QSEAARKASRDIDFGRLFRRVSPMDVAVATRQLSVLIRSGVPLVEALSALIEQLDQPE
                     LKAAFTDTRNQVNEGTNFADALKAHPKIFLTLYVNMVAAGEASGTLEEVLARLAEFLD
                     EQTRLQSKVRGALAYPVVMAVVVVLILFLMMSVVVPKVTSIFENFNQTLPWYTSLLIW
                     VSNVFSNYWWLLGALIGGGVYWFRRWKATTEGRRKWDLFVLEMPLFGPLLIMVAVSRF
                     ARTLATLLASGVPVLTAMDITRNVLGNTELMRVVEEARASVSEGEGIAKPLRQAGRFP
                     PIVTHMIAIGERTGQLEEMLVHVAEAYDQQIEVRVGAMTSILEPLLIVVMGVVVGGIA
                     FAILMPLLQLNEMIQ"
     gene            2683..3105
                     /locus_tag="VFG22_00845"
     CDS             2683..3105
                     /locus_tag="VFG22_00845"
                     /inference="COORDINATES: protein motif:HMM:NF019934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HET6414810.1"
                     /translation="MARRGRRQRVALPWERRGGRWRGAFRNIRWQALLGGVLLLMLAF
                     GFTKYVRHRVRVRNTDVAVAQVKQAIDRFRADVGRCPSSAAELLHPPLSQKHYLASMP
                     KDGWGGPLTIRCPGLFEEEAEVISAGPSGSLLKDDNIQ"
     gene            3123..3518
                     /locus_tag="VFG22_00850"
     CDS             3123..3518
                     /locus_tag="VFG22_00850"
                     /inference="COORDINATES: protein motif:HMM:NF019934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HET6414811.1"
                     /translation="MPNTTDKLQHRRLARQTGREGFTLIEIMIVITIFAMMAGGVAVA
                     LLPQLEKARIKTTKTDAEALRSAVMLYKADNPRACPTVEDLVTERYLDGTRRTTDAWD
                     TPFQISCEDGDISVISAGEDLEFNTEDDI"
     gene            3525..4214
                     /locus_tag="VFG22_00855"
     CDS             3525..4214
                     /locus_tag="VFG22_00855"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET6414812.1"
                     /translation="MLLRVPNRTKGGFTLIEVILVIVIIAIVITGATFGLGAITRTRL
                     RSSAFKVASAAKFAYNRSLTQGTTTRLQFDFEKNTMAVEETETPVTLATEEQLEDEPG
                     EAVDPWDLARTRLETPLSKVKPTSPFQPISNRDGEPLKQYTAQPLGDGIVIHGLITPH
                     ETEPRTDGEGAVYFFPGGVTEHAAIQLSDSSETVYSVEIHPLTGNARIHNFAYEPIDE
                     LDDDGEVRDSL"
     gene            4211..4828
                     /locus_tag="VFG22_00860"
     CDS             4211..4828
                     /locus_tag="VFG22_00860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET6414813.1"
                     /translation="MTRRGFTLLEVMIAVAILGLSLTAIFSSEVGAANVAARARRQNV
                     AATLARCKMGEIEQLIQIEGLPAIEKKDSDNCCEHAPQEGFECEWIVERIILPEFGTQ
                     DDEDDGTSEQDTPNQRLTEAYDEVKSEGGTPQQVIAGQAGNLTMMALQIGFPILKPFL
                     EEQVRRATVTVRWKEGPSERGFDVIQYLVSEQPAPVDADALQGEP"
     gene            4825..5508
                     /locus_tag="VFG22_00865"
     CDS             4825..5508
                     /locus_tag="VFG22_00865"
                     /inference="COORDINATES: protein motif:HMM:NF023044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspJ"
                     /protein_id="HET6414814.1"
                     /translation="MNRGFTLLEIILAVTVLALVGTMVYGGFSQTALNKARVEEDVDQ
                     SRIVHMALDRMVRELTMAFVSTHVNPSLDLRVVETAFIGKDNGKDDRIDFTSFSHRRL
                     YRNARESDQNEISYFVTEHPDEPGVQVLARREQNRIDEDPRRGGKSQILVENVEEFNL
                     DYFDPLLSDWVQTWDTEDILAQPNRLPSQVRIRLSIKDPRHRGKTQTFGTRVTIPLTY
                     ALNHANYNP"
     gene            5505..>6230
                     /locus_tag="VFG22_00870"
     CDS             5505..>6230
                     /locus_tag="VFG22_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414815.1"
                     /translation="MNDASRKRRSKRSEEGIALIIAIATVAILSVMLTDMHETTGTAF
                     AVSTSQRDALQAEYMAKSAINLTRLLIAKEPEVRSFVNPLYKAATGRPAPQLPVWNFI
                     DELLSPFCTPEDQRSTLVELGIDFGNTVGFDDLPGECHVVAVSENGKINVNDPLFLDG
                     ELARNSVATQLFSLTGGHLPESPYDALFNKQDENGTLTTRIDLITAVIDWWDQDIQRT
                     DFDPGGGITRTGGTGTEDDSFYQM"
BASE COUNT         1410 a         1866 c         1816 g         1138 t
ORIGIN      
        1 tttccgatcg atgacctgag aatcttgctc ggaaagacga tcattcccgt agcggcggct
       61 gaagaaacca tcgacgacgc gatcaaccga gtctacgagc gcaaagacga gaccgcactc
      121 gccgaagcga aagaaggcga agaggtcgaa gagctgcgcg acctaatcga catgaccgac
      181 gaggcgccgg tgattcgctg ggtcaataat ctcttttaca ccgcagttaa ggagcgagcg
      241 tccgacattc acatcgaacc caccgacgag aaggtgatcg tgcgatatcg agtcgatggc
      301 aggctcatcc ctcgcaagga agcgaatcgt ggattcctct cgtcgattgt gagccgcatc
      361 aagatcgaag ccggtctcaa cattgcggaa aagcggctgc cgcaagatgg tcgcatcacc
      421 aagaagatcg cgggcaaggt cgtcgatgtt cgtgtctcaa ctattcccac tgcaaagggt
      481 gagcgcgtcg tcatgcgtct gctcgacaaa gagcaagtgc tactgaactt gatcgacctc
      541 gggttcgcac gtcgtgagct cgatcagctc gagcatctga tcgggcgtcc caatggaatc
      601 atcctggtta ccgggcccac cggctctggc aagacgacca cgctgtacgg ttgcctcaac
      661 aagatcaaca cacccgagaa gaacatactg accgcggaag atcccgtgga gtacgagttg
      721 cggggcattg gtcagatgca agtgcaaccg aagatcggtc tcaccttcgc ggcgggtctg
      781 agaagctttc ttcgtcagga tccggacgtc atcatggtcg gcgagatacg agaccacgaa
      841 acagccgaga tcgcgattca tgcatcgctc accggacacc tcgtgctttc caccttgcac
      901 acgaacgacg cgccaagcgc catcacgcga ctggtcgaca tggacataca ggcgtatcag
      961 atctcatcgt ccgtactcgc agtcctggcg cagcgcctgg tccgaaagct ctgcgatcat
     1021 tgcaagactc cctacgcgcc gactccacag gaccttcgcg agctcggcat cgacgcggac
     1081 agcgcgaagg agaagttggc agagcttcgg agggtggcgg cgacgacggt cattgggcac
     1141 ggcactccat cgacgttgcc gaaccccgcc aacgccagct ccgatgagct aaccttcttt
     1201 caccaccatg gttgcgatgc gtgcagcaat acgggctatc gcggacgaat cggcatcttc
     1261 gagctcatga tcatcgacga accggtccgg cgcgagatcc tcaacaacag cgatgcaaaa
     1321 acgattcaac gtgtcgcgca gcaccaggga atgcggcttc tgcgcgaaga cggcgcgcgt
     1381 caagtcgtgg caggcgtaac gagcgtagaa gaggtactcg cggccaccca agctgcagag
     1441 gtctaggcaa tggctgtcta tgcgtacaaa ggagtcaatg cgcgcggaaa atccgtcaaa
     1501 ggtatccgag acgccgatag cgccaaggct ctgcgcaccg ttctcaaacg tgacggagtc
     1561 ctcgcgacgg agatcctcga gcagtccgag gctgcgcgca aggcctcacg cgacatcgat
     1621 ttcggccgat tatttcgacg cgtctcgccc atggacgtcg ccgttgccac tcgacagctc
     1681 tccgtcttga tccgttctgg agttcccctc gtcgaagccc tgagtgcgct gatcgagcag
     1741 ctcgaccaac cagagctcaa agccgcgttt acggacacac gcaaccaggt caatgagggc
     1801 accaacttcg ccgatgcgct caaggcgcat cccaagatct tccttacgct ctacgtcaac
     1861 atggtagccg cgggtgaggc gtccggtacg ctcgaagagg tcctcgcacg gctcgccgag
     1921 ttcttggacg agcagacccg cctccagagc aaagttcgcg gcgcgctcgc atacccagtc
     1981 gtcatggcgg tcgtagtggt cctcatcctg ttcctgatga tgtcggtcgt ggtgccgaaa
     2041 gtcacttcga tcttcgagaa cttcaaccag acactcccct ggtacaccag cctgctgatc
     2101 tgggtgagta acgtcttcag caactactgg tggctcctcg gtgccctcat cggcggtggc
     2161 gtgtactggt tccgacgctg gaaggccacg accgaagggc gcagaaagtg ggatctgttt
     2221 gtcctcgaaa tgccactttt cggcccgctg ctcatcatgg tcgcggtgtc gcgctttgcg
     2281 agaactttgg caacgcttct cgccagcggt gttccggttc tcactgccat ggacatcacc
     2341 cgcaacgtcc tgggcaacac ggagctcatg cgagtcgtcg aggaagcgcg ggcgagcgtg
     2401 agcgagggag aaggaatcgc caaaccgctc cgacaggcgg gaaggtttcc gccgatcgtg
     2461 actcacatga ttgcaatagg tgaacgcacc ggtcagttgg aggagatgct ggtgcatgtg
     2521 gcagaagcct acgaccaaca gatcgaagtg cgcgtcggag cgatgacttc cattctcgaa
     2581 cctttgctga tcgtggtcat gggcgtggtg gttggtggca tcgcgttcgc cattctcatg
     2641 ccactgctac aactcaacga gatgattcaa tgaaagctga gcatggcgcg aagaggacga
     2701 agacaaagag ttgcgctgcc ctgggaacgt cgcggtggtc gttggcgagg cgcgttccgc
     2761 aacatcaggt ggcaagccct gctcggcgga gtgctgctcc tcatgcttgc tttcggattc
     2821 acgaagtacg tgaggcatcg agtccgagta cggaacaccg atgtcgcggt agcgcaggtg
     2881 aaacaggcga tcgatcgatt ccgcgccgac gtgggccgtt gtccaagctc ggcggcggag
     2941 cttctgcacc ctcccctttc gcagaaacac tacctcgcgt cgatgccgaa ggacggatgg
     3001 ggggggccgc tcacgattcg ttgcccaggc ctcttcgagg aagaagccga agtgatttcc
     3061 gcgggcccaa gcggctcgtt actgaaggac gacaacattc aatgatgcca actggaggct
     3121 ccatgcccaa cacgactgac aaactccaac accgacgact ggcccgccag accggccgag
     3181 aaggtttcac gctcatcgag atcatgatcg tcatcacgat cttcgcgatg atggctggcg
     3241 gggttgccgt cgcgctattg ccacagctgg agaaggcgcg aatcaagacg accaagacgg
     3301 acgctgaggc gctgcgctcc gccgtcatgc tctacaaggc cgacaacccg agagcttgtc
     3361 ccacggtcga agatctcgtg accgagcgat atctcgacgg aactcgcaga acgaccgacg
     3421 catgggatac gccgttccaa atcagctgtg aggacggcga catctccgtg atttctgcag
     3481 gagaggacct cgaattcaac actgaagacg acatctaagg atccatgctg ctacgcgtcc
     3541 ccaaccgaac caaaggcgga ttcactctca tcgaggtgat tctggtaatc gtgatcatcg
     3601 cgatcgtgat tacgggggcg actttcggcc taggcgcgat cacacgaacg cggctgaggt
     3661 cgtcagcatt caaggtcgcg agcgccgcca agtttgcata caaccgttcc ctcacgcagg
     3721 ggacgaccac gcgcctacag ttcgacttcg agaagaatac gatggcagtc gaggaaaccg
     3781 aaacaccggt gacgctcgca accgaagagc agttggaaga cgagcccggg gaagcggtcg
     3841 acccatggga cttggcccga acccgactcg aaacacctct gagcaaggtg aaaccgacat
     3901 cgccgttcca gcccatctcc aaccgggacg gcgagccact caagcagtac acggcgcagc
     3961 cgctcgggga cgggattgtg attcatggct tgatcactcc gcatgagacc gaacctcgaa
     4021 ccgatggcga gggcgcggtc tatttctttc ccggcggtgt cacagagcac gcggccatcc
     4081 agctcagtga ctcgagcgaa acggtctaca gcgtcgagat tcaccctctc acgggtaatg
     4141 cgcggattca caattttgct tacgagccaa tcgatgagct ggacgacgac ggcgaagtga
     4201 gggattcgct gtgactcggc gcggattcac gctcctggag gtgatgattg ccgtcgcgat
     4261 cctcggactt tcgctgacgg cgatcttctc gagcgaggtc ggggccgcaa acgtggcggc
     4321 tcgcgcccgg cgacagaacg tcgccgcaac cctcgctcgc tgcaagatgg gcgaaatcga
     4381 acagctcatc caaatcgagg gtctcccagc gatcgagaag aaggacagcg acaactgctg
     4441 cgagcatgca cctcaggagg gtttcgaatg cgaatggatc gtcgagcgaa tcatacttcc
     4501 ggagttcggt actcaagatg acgaggacga tggtacaagt gagcaggaca cgccgaacca
     4561 gcgccttacc gaggcatacg acgaggtcaa aagcgagggg gggacccctc agcaggtgat
     4621 cgcaggtcaa gcaggaaacc tgacgatgat ggcgctgcag atcggcttcc cgattctgaa
     4681 gccctttctg gaagagcagg tgcgcagggc cacagtgacc gtccgctgga aagaggggcc
     4741 gtcggagcgc ggcttcgacg tcatccagta cctggtttcg gagcagcccg ccccggtcga
     4801 tgctgacgca ctgcaggggg agccatgaat cgtggtttta cgctcttgga gatcatcttg
     4861 gcggtcacgg ttctcgcgct cgtgggcacg atggtctacg gcgggttctc tcagactgcg
     4921 ctcaacaaag cgcgcgtcga ggaagatgta gatcagtccc gaatcgttca catggcgctc
     4981 gaccgaatgg tacgcgagct caccatggca ttcgtttcaa ctcacgtgaa tccatccttg
     5041 gatctccgag tggtcgagac cgccttcatc ggaaaagaca acggcaagga cgatcgcatt
     5101 gatttcacct cgttctcaca tcggcgtctc tatcgaaacg cgcgggagtc tgaccagaac
     5161 gagattagct acttcgtaac cgagcacccc gacgagcccg gagtgcaagt cctcgcacgc
     5221 cgcgaacaga atcgaatcga cgaagacccc cgtcgaggtg gcaagtcaca gatcctcgtc
     5281 gagaacgtcg aggaattcaa tctcgactac ttcgacccac ttctcagtga ttgggttcag
     5341 acttgggaca ccgaagacat cctcgcgcag ccgaatcgcc tcccctcgca ggttcgaatt
     5401 cgtctgtcta tcaaggaccc gcggcaccgt ggaaagacgc agaccttcgg cacccgagtg
     5461 accattccac tcacctacgc cctcaaccac gcgaactaca atccatgaac gacgcgagca
     5521 gaaaacggcg cagcaagcgc tccgaggaag gcatcgccct catcatcgca atcgcgacgg
     5581 tggcgattct atcggtaatg ctcaccgaca tgcacgaaac gaccggtact gcgttcgccg
     5641 tttcgacgtc acaaagggac gcgcttcagg ccgaatacat ggccaagagc gccatcaatc
     5701 tcacgcggct gctgatcgcg aaggaaccag aggttcgaag cttcgtcaat ccgctctaca
     5761 aagccgcaac cggtcggccg gcgcctcaac tgcccgtgtg gaacttcatc gacgagctct
     5821 tgtccccgtt ctgcacacct gaagaccagc gcagcacgct ggttgaactc gggatcgact
     5881 tcggaaacac ggtcgggttc gacgaccttc cgggtgaatg ccacgtcgtg gcagtcagcg
     5941 aaaacggcaa gatcaacgtc aacgaccctc tgttcctcga tggtgagctc gcacgaaaca
     6001 gcgtggccac ccagctcttt tctctgacgg gagggcacct tcccgagagt ccgtacgacg
     6061 cgcttttcaa caagcaggac gagaacggca cgctcaccac ccgcatcgac ctcatcacgg
     6121 cggtcatcga ctggtgggat caggacatcc agcgcactga tttcgaccct ggcggtggca
     6181 taacccgaac cgggggcacc ggaacggagg acgattcgtt ctaccagatg
//
LOCUS       DASRZF010000016         4383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1043753, whole genome shotgun sequence.
ACCESSION   DASRZF010000016 DASRZF010000000
VERSION     DASRZF010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1043753"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VFG22_00875"
     CDS             complement(<1..482)
                     /locus_tag="VFG22_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414816.1"
                     /translation="MNYQEFLSSKTERPSHSPLSRTSGFGESLFPFQRDIVEWAARLG
                     RAAIFGDCGIGKTLIQLEWASADDGITLIAAPLAVSDQTIAEGERFGIEVKRARDIDD
                     VHESCGIWITNYERLGKFGALGDTFSSIVLDESSILKSFAGKTRNAIIAQSLGVPHRL
                     A"
     gene            complement(830..1210)
                     /locus_tag="VFG22_00880"
     CDS             complement(830..1210)
                     /locus_tag="VFG22_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET6414817.1"
                     /translation="MKAKRLDPWERILIQRRRDGVSQREAADACGAKSLYQFRLWESG
                     TEAPPAEVLQNADPRDLKIHERCYLERVRRGISLKALADELGVSRWWLCQMERGKVSS
                     RQLEHRWRRELRPWRGAPVEMARG"
     gene            complement(1269..>4383)
                     /locus_tag="VFG22_00885"
     CDS             complement(1269..>4383)
                     /locus_tag="VFG22_00885"
                     /inference="COORDINATES: protein motif:HMM:NF024939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HET6414818.1"
                     /translation="WLRKYTRRKRIYYGWLVLLSCAWLWYLTMCSGGSAPASDLQGLP
                     GDLSSAPVHQAIWVQLILFVVSIVLSELLRPKPNLEDARPAGLGDFQFTTATEGRPVP
                     IMCGTNLMKGPNVVWYGDLKQSAIKETVSTGLWSSETFIKGFRYHIGVQMVLCRGSDD
                     APCTLLRVFVGEEEIYSGTVSGPEGRFDIDRPFFLGGDEFGSGGIQATVDFYSGTSTQ
                     PISAYLDDPDRQQIATAATPTAPRYSKSCHVVIRKLTGAAPSASNSGAYIGNSTTIGH
                     WSFEMQRFQSRFTGQALGENIIGTYDANPINFAYELLTDTEWGFGFDPNTIDIGVGST
                     FLKASDNMIAEGNGMSWVIDRQIPAQQVLTEIERQIEGKVFLSPFSGKWEIQLIRGPD
                     DTNFGYDVNTVPQVTDDELESIKDYTRGSWEDTTNNIQLKYDKRDDDYKESYAIAQDP
                     ANAILLAAGSYLDPVSQSGIVAFPGVKSSALAANLVWRELRGQCRPLARGTFIVNRKL
                     YTLTIGGVFAWTSAKFGYTKLAMRATRIDYGTLTDNKMTITAVEDAFQFAAASYGTPP
                     ATGWTPPSVGLAAYPADEQVAFEAPRGIITREPSYQGNPDVSKVWCGARQQGSEVGFT
                     IGQRNASGVPGGSYADAGSSISFLYIGQLDADLVAGVANPTSSILINSTPDDQTTLEA
                     LFDDTATAEDLGVGLVQLCLVNDEFFLVSSASTSGADVALENVYRGVLGTGQANHSAG
                     DDVFLIFAGGNLTTTVFPDTNNVDVELRSRSSQAVYAGATTAVSLTMAQRAMRPYPPN
                     AALYNGGSTPFTAPDLEGDGSGLNGVGFDIDWRRSRYTAGDEVAELGADNTPDASTEH
                     RVRVYVAPAGANDLAFDSGWVSGTGPETPTQAELVTFAAAGTEIEVQIDSRHDIGTLT
                     NLESWTPLLHRVTPTSTRSSQFYIGGNLSASTASTAYVAVSATVHNVTIGAAYSTSDV
                     EAQINGGGWSVVIAASGTTGALNGGGALSISDSVELRHTVNETPDPQFVEIDDGTNPV
                     AYGAFSA"
BASE COUNT          823 a         1410 c         1325 g          825 t
ORIGIN      
        1 gcaagtcgat gtggaacgcc taaagattgc gcaatgatcg cgtttcttgt ctttccagcg
       61 aatgacttca ggatcgaact ttcatccaga acgatagatg agaacgtgtc tcccagcgcg
      121 ccaaactttc caagtcgctc atagttcgta atccagatgc cgcacgactc gtgaacgtcg
      181 tcgatatcgc gtgcgcgctt gacctcaatt ccgaatcgct cgccctccgc gatggtctgg
      241 tcggacactg cgagcggcgc agcgatgagc gtgatgccat cgtccgcgct tgcccactcg
      301 agttgaatga gcgtctttcc gatcccgcag tcaccgaaga tcgcggccct gccaaggcgc
      361 gcggcccatt ccacgatatc gcgctgaaac ggaaaaagag actcgccgaa tccagacgtg
      421 cgcgacaatg ggctgtgtga tggacgctct gtcttggagg aaagaaactc ctgatagttc
      481 acatcaagtc cccaaaaaaa agaagcgccc gggagggaag aacggcttgg tggagaggtt
      541 ttacggcaga catgccaacc cgagcgctgt ggagattttg cgaacaggca aggcgccttt
      601 agtttgccgc cggtgacgcc ccccttatcg cccgtccgca tcggtcctgc gtgtgtctgt
      661 ttcagggtga cgccggaccc agcatcggag ggaagtgaga gtccacggcg actggctcgc
      721 caaaggtgcg ccgtggactc tctgagagca ggagccctca accagatcat gcgtcgacgt
      781 ggaagatagc ccggatcgaa aaaaggacaa ccgggaaagt gccgcggaat tatccgcgcg
      841 ccatctccac gggagcacca cgccacgggc gcagctcgcg tcgccagcga tgctcaagtt
      901 gtctcgagct caccttgccg cgctccatct gacagagcca ccagcgcgac actccgagtt
      961 cgtcggcgag cgccttgaga gagatcccgc gtcggacgcg ctcgaggtag caccgctcgt
     1021 ggatcttgag gtcgcgcggg tccgcatttt ggagaacttc cgccggcgga gcctccgtgc
     1081 ccgactccca gagccggaac tggtagaggg acttcgcgcc gcacgcatcc gccgcctcgc
     1141 gctgggagac cccatcgcgc cgacgctgga tcaggatccg ctcccaggga tcgagtcgct
     1201 ttgctttcat gcgaccatga tagcgtgcgc aagctgtgat atatgttgca tttatgctga
     1261 taccgaacct aggctgaaaa cgccccatag gcgaccgggt tcgtgccgtc gtcgatctcc
     1321 acaaactgcg gatccggtgt ctcattgacc gtgtgccgaa gctccaccga atcgctgatg
     1381 ctgagcgcac ccccgccgtt gagcgccccc gtcgtgccgc tggcggcgat cacgaccgac
     1441 cagccgccgc cgttgatctg cgcctcgaca tccgaggtcg aataggcggc gccgatggtc
     1501 acgttgtgta ccgtcgcaga gaccgcgacg taggccgtgg acgcggtcga cgcactcagg
     1561 ttgccaccga tgtagaactg actcgatcgt gtcgaggtgg gcgtcacacg atgcaggagc
     1621 ggcgtccagc tctccaggtt cgtcagggtg ccgatatcgt gccgcgagtc gatctggacc
     1681 tcgatctcag tccccgccgc cgcgaatgtc acgagttccg cctgagtcgg cgtctccggc
     1741 cccgtgcctg aaacccagcc ggagtcgaag gccagatcgt tcgctccagc aggcgcgaca
     1801 taaacccgca cccgatgctc ggtagatgcg tccggagtgt tgtccgcacc cagctcggcg
     1861 acctcgtccc ccgcggtgta gcggcttcgg cgccagtcga tgtcgaaccc gacgccgttg
     1921 agccccgagc cgtcgccctc gagatccggc gccgtgaaag gcgtgctgcc cccgttgtag
     1981 agcgccgcat tcggcggata gggacgcatg gcccgctgcg ccatcgtcag ggaaaccgcc
     2041 gtcgtggcgc cagcgtagac cgcctgagaa gaacgagatc gcagctcgac atcgacgttg
     2101 ttggtgtcgg ggaagacggt agtggtgagg ttcccgcccg cgaagatcag gaagacgtcg
     2161 tcgcccgccg agtggttcgc ctgccccgtc cccaggaccc cacggtacac gttctccagc
     2221 gcgacgtccg caccgctcgt gctcgcgctc gacaccagga agaattcatc gttcactagg
     2281 cagagttgca cgagccccac gccgaggtcc tccgcggtcg cagtgtcatc gaacaacgcc
     2341 tcgagcgtcg tctgatcgtc gggcgtcgag ttgatgagga tactcgatgt cggatttgcc
     2401 acgccggcca caagatccgc gtcaagctgc ccgatgtaga ggaacgagat cgaactccct
     2461 gcgtcagcgt aggacccgcc cggcacacca gaggcgttgc gctgtccgat cgtgaatcca
     2521 acctcggagc cctgttggcg cgcgccgcac cagaccttcg acacgtcggg gttgccctga
     2581 tagctcggct cccgcgtgat gatcccacgc ggcgcctcga acgcaacctg ctcgtctgct
     2641 ggatacgctg caagccccac gctcggcgga gtccatcccg tggccggggg cgtaccgtag
     2701 gatgctgcgg caaactggaa cgcatcctcg accgccgtga tggtcatctt gttgtccgtc
     2761 agcgtcccgt agtcgatccg tgtcgcacgc atcgccagct tcgtgtaacc gaacttcgcg
     2821 ctcgtccatg cgaagacacc gccgatcgtg agcgtgtaca gcttgcggtt cacgatgaag
     2881 gttccgcgcg caagtggtcg gcactggccg cgcagctcac gccagaccag attcgcagca
     2941 agcgcagaac tcttgacccc ggggaacgcc acgatgccag attgggacac gggatcgaga
     3001 taggaccccg ctgccagcag gatcgcattc gccgggtcct gcgcgatggc gtagctctcc
     3061 ttgtagtcgt cgtcgcgctt gtcgtacttg agctggatgt tgttcgtcgt gtcttcccac
     3121 gatccgcgcg tgtagtcctt gatcgactcc agctcatcgt ccgtcacttg cggcacggtg
     3181 ttcacgtcgt agccgaagtt cgtgtcgtcc ggcccacgga tgagctggat ctcccacttg
     3241 ccggagaacg gactcaagaa aaccttcccc tcgatctgcc gctcgatctc cgtgagcacc
     3301 tgttgagccg ggatctgtcg gtcgatcacc caggacatgc cgttcccttc ggcgatcatg
     3361 ttgtccgacg ccttcaggaa cgtggagccg acgccgatgt cgatggtatt cggatcgaag
     3421 ccgaatcccc actcagtatc cgtcagcagc tcataggcaa agttgatggg gttcgcgtca
     3481 taggtgccga tgatgttctc gccgagagcc tgtccggtga atcgagactg gaaacgctgc
     3541 atctcaaacg accagtgtcc gatcgtcgtc gagttgccga tgtaggctcc tgaattactc
     3601 gcgctcggcg ccgcgcccgt gagcttccgg atcacgacgt gacaggattt cgagtacctt
     3661 ggtgctgtgg gagtcgctgc cgtcgcgatt tgctgccggt ctggatcatc cagataggcg
     3721 ctgatcggct gcgtcgatgt gcctgagtag aagtcgaccg ttgcttggat gccgccggac
     3781 ccgaactcat cgccgcccag gaagaacggg cgatcgatgt cgaagcgccc ttcggggccg
     3841 ctcactgtcc ccgagtaaat ctcctcctca cccacgaaca cgcgcagcag cgtgcatggc
     3901 gcgtcgtcac tgccgcgaca cagcaccatc tgcacgccaa tgtgatagcg gaagcccttg
     3961 atgaacgtct cggacgacca gagcccggtc gagacggttt ccttgatcgc cgattgcttg
     4021 aggtcaccgt accagactac attcggaccc ttcatgaggt tcgtgccgca catgatcggc
     4081 accgggcgcc cctcggtcgc ggttgtgaac tggaagtcac cgagccccgc gggacgcgcg
     4141 tcttccaagt tcggcttcgg acgcagcagc tccgagagca cgatcgatac gacgaacagg
     4201 atgagctgca cccagatcgc ttgatgcacg ggagcgctgg ataggtctcc gggcaaccct
     4261 tgcagatcgc tcgcaggagc cgagcccccg ctacacatcg tcagatacca gagccacgcg
     4321 cacgatagca gcacgagcca gccgtagtag atgcgcttgc ggcgcgtgta cttgcgcagc
     4381 cat
//
LOCUS       DASRZF010000017         3166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4139467, whole genome shotgun sequence.
ACCESSION   DASRZF010000017 DASRZF010000000
VERSION     DASRZF010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4139467"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VFG22_00890"
     CDS             <1..465
                     /locus_tag="VFG22_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414819.1"
                     /translation="TYEASGTVSGFVNSFASSIINSALISQITQIVINNYWDTPLDIP
                     GVISRSTASAVSLIRQRISNALSAAIATLGLMDLTNAYMNSVVGVPNDGLVPRNSTEL
                     AGAIHRELSSGDHASPVMDVDPHKNFWTQDHRNTVTSGLIEEVRDLAGVGGE"
     gene            complement(486..1547)
                     /locus_tag="VFG22_00895"
     CDS             complement(486..1547)
                     /locus_tag="VFG22_00895"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HET6414820.1"
                     /translation="MGGRSKRGHGAGGDKTLDASHLRPSIHPTYARLLVAELKRRGFA
                     DADILEGTAQAPAHLLEEERFLNFEQFRRLALRGTALTGQPWLGLDVGRSTQISSHGP
                     LGYAMVASANVSQTLDVVARYSALRLQITEFSVERDDGRVQLRIEDTLGWGDLDEYIA
                     SHIVGALCLLLETVSGMPLPETRVGFTYPAPDWASEYATRLRGVTLEFDAPICVIDMP
                     ESFAATPCITADGGAFRQALRLCERAEAQHRSDGDVAQRVLDRLLDQEGEYPSLSEVA
                     KAVYMSERTLNRKLKAQGTSYQMLLDDVRQELAVWYLRETEMPVEAIAERLGYRDTSN
                     FSRTCKRWFGVTPREIRGG"
     gene            complement(1618..2835)
                     /locus_tag="VFG22_00900"
     CDS             complement(1618..2835)
                     /locus_tag="VFG22_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HET6414821.1"
                     /translation="MSPLNPVFSVENVRYQDGKRYAWLVSLLVPAALGCAPLLYALTG
                     SVASLWIPALTIYGTIPLLDLLLGEDRNNPPEEVVPLLEADSYYRWATYALVPVLWAT
                     FFANAYFVGTHSLPWYGMLAMVVNTGILGGFAINLGHELGHKKSKLERNLATAALSMS
                     AYGHFAIEHNRGHHRHVATPEDCTSSRMGETLYRFAARELPGAFRRAWTLEAGRLERH
                     DQKVWSLQNEIVRAGLITLGVTVALVVAFGPVMIPYLLASYFVGGFHLTMANYIEHYG
                     LLRQKRANGLYERCQPHHSWNSNHICSNWALYHLQRHSDHHANPGRRYQSLRHFEGLP
                     TLPNGYFGMFLLSLVPSLWFRVMDPILLAHADRDPARIHFDPKRREELCARYGLEDRL
                     PAEEAPATARAAA"
     gene            complement(2950..>3166)
                     /locus_tag="VFG22_00905"
     CDS             complement(2950..>3166)
                     /locus_tag="VFG22_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414822.1"
                     /translation="GSFYSIDMLPPFWQKVTLLNPVVYLISAFRWSFYGLADVSVTLS
                     AGMILLFLGVCLLLVRWMFKSGYRLRA"
BASE COUNT          632 a         1046 c          959 g          529 t
ORIGIN      
        1 acctatgaag ccagcggcac cgtctccggc ttcgtgaaca gcttcgcgag cagcatcatc
       61 aactccgcgc tcatctcgca aatcacccaa atcgtcatca acaactactg ggacacgcca
      121 ctcgacatcc caggcgtcat cagccgctcc accgcatcag ccgtcagtct cattcggcaa
      181 cgcatcagca acgcgctttc cgcggccatc gcgaccctcg gcctcatgga cctgacgaac
      241 gcgtacatga acagcgtcgt cggtgtcccc aacgacggcc tggttccgcg caacagcacg
      301 gagctcgcgg gggccattca ccgggagctc agctccggtg accacgccag cccggtcatg
      361 gacgtcgacc cacacaagaa cttctggact caagaccatc gaaacaccgt cacttcgggg
      421 ctcatcgaag aagtgcggga tcttgcgggc gtcggcgggg agtagcgagc gcgctcgacg
      481 cagcctcagc ctccgcgaat ctcccgcgga gtcaccccga accagcgctt gcaggtccgg
      541 ctgaagttcg acgtgtcccg atacccaagg cgttctgcaa tcgcctccac cggcatttcg
      601 gtctcacgca ggtaccaaac tgcgagctcc tgacgcacgt catcgagaag catctgatac
      661 gaggttccct gggccttgag ctttcggttc agcgtgcgct ctgacatgta caccgctttc
      721 gccacctcgc tcaggctcgg gtactcacct tcttggtcga gcagacggtc gagcacccgc
      781 tgggccacgt cgccatcgct tcgatgctgc gcctccgctc tctcgcagag ccgcagtgct
      841 tgccgaaacg cgcccccgtc cgccgtgatg cagggcgttg ctgcaaacga ctccggcatg
      901 tcgatgacgc agatcggtgc atcgaactcg agcgtcaccc cgcgaagccg cgtcgcatac
      961 tcgctcgccc agtcgggcgc agggtaggtg aaccccaccc gcgtctcggg cagcggcatc
     1021 ccgctcaccg tctcgagcaa gaggcagagc gcgccgacga tgtgcgaggc aatgtactca
     1081 tcgagatccc cccagccgag cgtgtcttcg atgcgcaact gcacgcggcc atcgtctcgc
     1141 tcgaccgaga actccgtgat ctggagccga agcgccgagt accgcgccac cacgtcgagc
     1201 gtctggctga cgttcgcgct ggcgaccatt gcgtatccga gcggcccgtg agaggagatt
     1261 tgcgtcgaac gcccgacatc gaggccgagc caaggttggc cggtcagcgc cgtgcctcgc
     1321 agggccaagc ggcgaaactg ttcgaagtta aggaatcgtt cctcttcgag aagatgcgcg
     1381 ggcgcctgtg ccgtgccttc gaggatgtcc gcgtcagcaa acccgcgtcg cttgagctcc
     1441 gcgacgagca gccttgcgta ggtcggatgg atgctcggtc gaaggtgcga cgcatcgagc
     1501 gtcttgtccc cgcccgcgcc gtgccctcgt ttgcttcgac cgcccatctt cgtagcccac
     1561 gaagataggg cggccgttca aacgaatcaa cgcccgtgcg agcaccctgg gctcgactca
     1621 agccgccgcg cgagccgtcg ccggggcttc ttcggccggc aatcggtctt cgagcccgta
     1681 tcgagcacaa agctcctcac gccgtttcgg atcgaagtgg atgcgcgccg ggtcccgatc
     1741 ggcgtgcgcc agcaggatcg gatccatcac gcggaaccaa agcgagggca ccagtgagag
     1801 caagaacatc ccgaagtacc cgttcggcaa ggtcggcagt ccctcgaagt gtcgaagcga
     1861 ctgatacctg cgtccggggt tcgcgtggtg gtccgagtga cgctgcaagt gataaagcgc
     1921 ccagttcgaa cagatgtggt tgctgttcca ggagtggtgc ggctgacagc gttcgtacag
     1981 accgttcgcg cgcttctggc gcaggagccc atagtgctcg atgtagttcg ccatcgtaag
     2041 gtgaaacccg ccaacgaagt acgaggcgag cagataagga atcatcacag gtccgaacgc
     2101 cacgacgagc gccaccgtaa cgccaagcgt aatgagcccc gcccgaacga tttcgttctg
     2161 gagcgaccag actttctggt cgtgccgttc gagccgcccc gcttcgagtg tccaggcccg
     2221 gcgaaacgca cccggaagct cgcgcgccgc aaagcggtag agcgtctcgc ccatgcgcga
     2281 cgaggtacag tcttcaggcg tcgcgacgtg ccggtggtgg ccccggttgt gctcgatggc
     2341 gaagtgcccg tacgcgctca tggagagcgc cgcggtcgcc aggttccgct cgagcttcga
     2401 cttcttgtgc ccgagctcgt ggccgaggtt gatggcaaac ccacccagaa tcccggtgtt
     2461 gaccaccatc gcgagcatgc cgtaccaggg cagcgagtgc gtccccacga agtacgcgtt
     2521 tgcgaaaaag gtggcccaga ggacgggcac cagcgcatag gttgcccagc ggtagtacga
     2581 atcggcctcg aggagcggca ccacctcttc gggggggttg ttccggtctt ccccgaggag
     2641 caggtcgaga agggggatgg tgccgtaaat cgtcagcgct ggaatccaca gcgacgccac
     2701 cgagcccgtc agcgcataga ggagcggcgc gcagccaagc gccgccggca cgagaagcga
     2761 aacaagccag gcgtagcgct tgccatcctg gtagcgcaca ttctcaacgg aaaatacagg
     2821 gttcagtggg gacatcgatc aacctccggg gcgcagggcc tcaaaatctg tcccgaagat
     2881 gggcgatttt ggcctcttcc aacaccgagg ggcgcgacaa taaatcgtca attattgcca
     2941 gattgcggct caagcgcgaa gccgataccc gctcttgaac atccagcgca ccagcagcag
     3001 gcacaccccg agaaagagca gaatcatgcc ggcgctcaag gtcacgctca catccgcgag
     3061 cccgtaaaag ctccagcgga acgcgctgat gagatagacc accgggttga gcagcgtcac
     3121 cttctgccaa aacggcggca gcatgtcgat ggagtagaag ctcccg
//
LOCUS       DASRZF010000018         2869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4484338, whole genome shotgun sequence.
ACCESSION   DASRZF010000018 DASRZF010000000
VERSION     DASRZF010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4484338"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VFG22_00910"
     CDS             <1..389
                     /locus_tag="VFG22_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414823.1"
                     /translation="PITRIAIKYFLVPHLERQKALVHWLNPDAWRLSQVLQSNPQTTE
                     IYKKYNVWPMRLWALVSLAPHSYLMAICAMFNRLDVYLFIRVFVMNGIFLVAAIWQRH
                     ATCETIDELVGVDAIQGRDTGHPVAT"
     gene            complement(465..1778)
                     /locus_tag="VFG22_00915"
     CDS             complement(465..1778)
                     /locus_tag="VFG22_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HET6414824.1"
                     /translation="MAPPIVKPPKGKLGVLLPGIGAVGTTFIAGALLARKGLGLPVGS
                     LTQMGTLRLGKRTDNRSPLIRDFVPLADMNDLVFGGWDLFPGNAYDAAVTAEVLTAEH
                     LNAVKEELCAIKPMQSVFFPEFVKRLHGENVKPSQDKMELAEAVREDIRRFKKDNGLD
                     RCVAVWCGSTEVYREPAKAHSSLHEFEEGLRRSDPAISPSQVYAYACLREEVSYSNGA
                     PHLTVGEVPALLELAKETQTPVSGKDFKTGQTLMKTILAPGLGSRMLGIKGWFSTNIL
                     GNRDGEVLDDPESFKSKEVSKLGVLDTILQPELHPELYGDIYHKVRINYYPPRGDQKE
                     GWDNIDIFGWLGYPMQIKVDFLCRDSILAAPLVLDLALFMDLSHRAGLSGIQEWLSFY
                     YKSPITPKGLYPENNLFVQLGKLKNTLRWMQGEDLITHLGREYYD"
     gene            1863..2624
                     /locus_tag="VFG22_00920"
     CDS             1863..2624
                     /locus_tag="VFG22_00920"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HET6414825.1"
                     /translation="MTTPQIRNRAVILAAGLGSRLASGDLAPKPLRDVAGVPLIVRII
                     RALERGGVGEIGIVIGYLGEELKNGLSEHELRVKLSFFDNDEYEKPNGTSLLKARDFI
                     TGPTLLLMSDHLWSPGLLKSVGSYPVGPGESVLGVDYRIDDCFDLDDATKVQVQGDRI
                     ERIGKSLDTYNALDTGLFLITPALIEALDAVNGPEGCSLSEGVAALASKGRMKAADVG
                     DATWIDVDTPEAHAEAERLLAELGEDLTGAAPVTQ"
BASE COUNT          572 a          856 c          871 g          570 t
ORIGIN      
        1 gtccgattac gcggatcgcg atcaagtact tcctcgtgcc ccatctggag cggcagaaag
       61 cactggttca ctggctcaac ccggacgcct ggcgccttag ccaggtgctc caaagcaacc
      121 cacaaaccac cgaaatttat aagaaataca acgtttggcc gatgcgcctc tgggctctgg
      181 tctccctcgc cccgcatagc tacttgatgg cgatttgcgc gatgttcaat cgactcgacg
      241 tgtacctttt catccgggtc ttcgtgatga acggcatatt cttggtcgcc gccatctggc
      301 agcgccatgc aacatgcgag accatcgacg agctggtcgg agtcgacgcg attcaaggca
      361 gagacactgg gcaccctgtc gctacgtgaa tgttttgcaa cagtaccgtg aacaccgttc
      421 acagttgtga atgctgaacc acggcgtcgc tacctcccaa agggtcagtc gtagtattcg
      481 cgacccagat gggtgatcag gtcttcgccc tgcatccagc ggagcgtgtt cttgagcttt
      541 ccgagctgaa cgaagaggtt gttctcggga tagaggccct tcggtgtgat cgggctcttg
      601 tagtagaagg acagccactc ctgaatgccg ctcaaccccg ctcgatgcga gaggtccatg
      661 aagagggcca ggtcgagcac caagggcgcc gcaaggatcg aatcacggca gaggaaatcg
      721 accttgatct gcatcggata gccgagccac ccgaagatgt cgatgttgtc ccagccctct
      781 ttctggtctc cacgaggcgg ataataattg atgcgcacct tgtggtagat gtcgccgtag
      841 agttcggggt ggagttccgg ttgcaagatg gtgtcgagga caccgagctt gctgacttcc
      901 ttcgacttga agctctcggg atcgtcgaga acctcgccgt cgcgattccc gaggatgttg
      961 gtggagaacc atcccttgat gcctagcatt ctcgaaccga gacctggggc caagatagtc
     1021 ttcatcaggg tttgtccggt cttgaagtcc ttgccagaaa caggcgtttg agtctccttc
     1081 gcgagctcca acagggcggg aacctcgccc acggtgagat gcggcgcacc gttcgagtag
     1141 ctgacctctt cgcgcagaca agcgtatgca taaacttggg atgggctgat cgcgggatca
     1201 gaacggcgca aaccttcctc gaactcgtgc agagacgaat gtgctttggc gggctcgcgg
     1261 tacacctcgg tcgagccgca ccagacagca acgcagcgat cgagcccgtt gtccttcttg
     1321 aatcgtcgga tgtcttcgcg aaccgcctcc gcgagctcca tcttgtcctg actgggcttc
     1381 acgttttcac cgtgcaggcg cttgacgaac tcgggaaaaa acaccgactg catgggcttg
     1441 atcgcgcaga gctcttcctt gacggcattg agatgctcgg ccgtgagaac ctctgcggtg
     1501 acggccgcgt cgtaagcatt gcccggaaag agatcccacc ctccgaagac cagatcgttc
     1561 atgtcggcaa gcggcacgaa atcgcggatc agaggtgagc gattgtcggt tcgctttcca
     1621 agtcggagcg tgcccatctg cgtcaacgag ccaacgggca ggcccaggcc tttgcgagcc
     1681 agcagagcgc cagcgatgaa tgtcgtgccg accgcgccga ttcctggcag caacacgccg
     1741 agcttaccct tagggggctt tacgattggt ggggccatag tggcccaacg tatgccccac
     1801 tccggcgatg gatacaagaa gtggacgcgt tttctgcctc acgtgctagc gtcgagcgcc
     1861 cgatgacgac accccagatt cgaaaccgcg cggtcatcct cgctgcgggc ctcggcagta
     1921 ggctggcaag cggtgatctg gcgccgaagc ctctgcgcga cgtcgcgggc gttccgctga
     1981 tcgtgcgcat catccgcgcc ctcgagcggg gcggagttgg tgaaattggc atcgtcatcg
     2041 gatacctcgg cgaggaactg aagaatgggc tttcggagca cgagttgcga gtgaagctct
     2101 cgttcttcga caacgacgaa tacgagaagc ccaatgggac gtccctactt aaggcacggg
     2161 acttcatcac agggccgaca ctgctcctga tgagcgacca cctctggtcg ccgggtcttc
     2221 tgaaatcggt gggatcgtac ccggtcgggc caggggagtc cgtgctcggc gtggactatc
     2281 gaatcgacga ctgcttcgac ctcgacgacg ccacgaaggt tcaagtgcaa ggcgaccgga
     2341 tcgagcgaat tggcaagtcg ctcgacacgt acaatgcgct cgacacgggc ctattcttga
     2401 tcacgcctgc cctgatcgag gcgctcgacg cagtcaacgg tcccgagggc tgctcgcttt
     2461 ccgagggcgt ggcggctctg gcgtcaaagg gtcgaatgaa agccgccgac gtcggcgacg
     2521 cgacctggat cgacgtcgac acgcccgaag cccacgccga ggcggagcga ctgcttgcgg
     2581 agctcggtga ggacttaacc ggcgcggcgc cggtcacgca atagcgccgc tcgtctccgc
     2641 cgtcggacgc acgaacgctc acgatccgga cctcgcggcc gccgcgctgg cgcgtgagcg
     2701 gtcggaccag cattgccctg gacctgcgaa ttgatatact gttaaaatgc agtataagta
     2761 tatttccgct ctcttcctga cctcggtact ggccctctgc gctaccggat gcggcggaga
     2821 ctcgtcgggc gaaatccgag gcggcggcgg gacctcggga acgggctcc
//
LOCUS       DASRZF010000019         3857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3452830, whole genome shotgun sequence.
ACCESSION   DASRZF010000019 DASRZF010000000
VERSION     DASRZF010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3452830"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VFG22_00925"
     CDS             complement(<1..296)
                     /locus_tag="VFG22_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414826.1"
                     /translation="MLRNVFLISLSAALCGCNASSEGAASIDDEIFSAELFADPPSEF
                     RPQTRWWWPGGAVDDATLRDQLRSFVEVGYGAVEIQPFMSAITYADLREDALVRS"
     gene            complement(420..1418)
                     /locus_tag="VFG22_00930"
     CDS             complement(420..1418)
                     /locus_tag="VFG22_00930"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET6414827.1"
                     /translation="MGKTAFVTGGTGFIGINLVELLVREGWEVTALHRASSDLTYLER
                     FPVALAEGSIIDRESLERAIPDGTDVVFHVAGDTNFWSKRNARQTTNNVDGTRNMVEA
                     AAAKGVATFVHTSSNSAWGRVEGIVTEETPLRGKDSWINYERSKHLGELEALEGTKRG
                     LKVVVLNPGDVAGPYDVNSWGRIFTLLRDGALPAIPPGIISVTHVHDVVRAHLDAVTR
                     GRSGERYLLGGEDCSVADFVSEIARVSGTKAPRTAPAWVLKLYGRASVMVAAITGNEP
                     DVTPEIAAIGSRTGTTFRSDKAIRELGYRIRPMAAGVADNYAWLLKEGLLDAGMRS"
     gene            1574..2089
                     /locus_tag="VFG22_00935"
     CDS             1574..2089
                     /locus_tag="VFG22_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414828.1"
                     /translation="MGLLLLGSELATQAQNGKVPTPAELAGSYTYAGDRAKDEAAITA
                     KVNAATAEMSRMVLKRAKPKLVSSTGIPGQMDITPQAADLVFKMDDYVVAAPSNGSSA
                     KVTTPAGESADASFDSMSATLLQSVAKTGGAKAITFAFNEAGQLLAHVRITNDRLAGP
                     VTYTLLYARSK"
     gene            complement(2071..3225)
                     /locus_tag="VFG22_00940"
     CDS             complement(2071..3225)
                     /locus_tag="VFG22_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET6414829.1"
                     /translation="MFSRRGAFDLSPNVITRAAEATPPRWDLTHSNPTSAGIAFGDEA
                     MRAMQTASLDLYHPQPRGLASARQALGEHLDWNPDHLVLTATTSEAYSSLIKLFCDPG
                     DQVLIPEPSYPLLPMLARLDGVVALPYSFRYDGQWHIDRTAFAEGVGPEVKLILAVSP
                     NNPTGAYLSEEDLAFLSSQGVPLVVDEVFAPYDLRQAQPQPLRHSEARSGLLFALGGL
                     SKAAALPQLKLSWMAVSGEERLVKECLTQLDLITDTYLSVNEITQRALPDLLDASDGR
                     RRLVFERLQRNRRSLRELLRDAPETSALPPEGGWYQVLRLPNVLSEEEWVLRFLERGV
                     LVQPGWFYDFPEEAWVIVSLLTEPRVLSEGMRQVVEAQIEVLDSGYLERA"
     gene            complement(3292..>3857)
                     /locus_tag="VFG22_00945"
     CDS             complement(3292..>3857)
                     /locus_tag="VFG22_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414830.1"
                     /translation="ITAVQLQTTRSALGTAASEPTLEREAADFAKMIRGQDWATADPL
                     GLGGLLMDASRVAQLMQLGAFARGELLDDLLSSALPGLSYWNRQNDSGLPASQRLAFR
                     ELGLAIGLSGIELIDEAVGREDLALSAKTSEGLEALTPYAYLGSEIRSFWLDPEHREA
                     RTWSEHRDINEVMLATSLVPSGFLWIS"
BASE COUNT          752 a         1236 c         1168 g          701 t
ORIGIN      
        1 gaccgaacga gcgcgtcctc gcgcaggtct gcgtaggtga tggcagacat gaatggttgg
       61 atctcgacag ccccgtaccc gacctcgaca aagctgcgga gttgatcgcg aagggtcgca
      121 tcgtcgactg cacctcccgg ccaccaccaa cgtgtttgag gcctgaattc ggacggcgga
      181 tcagcgaaca attcggctga gaagatctcg tcgtcgatcg aagcggcccc ttcgctcgag
      241 gcgttgcacc cgcatagtgc ggcgctcaaa gagatcaaga atacgttccg gagcatcacc
      301 gttctttagc acggtccgga cggcggaagg ctctcggatt ttacgggcga ccgtccctct
      361 ttcgctcgag gttggcagcg ctcagctcga cgcgctgtcc gttcatgccg accccagact
      421 caagaacgca tgcctgcgtc gaggagacct tccttcagca gccaagcata gttgtcagcg
      481 acgcccgcgg ccatcggccg gattcgatag ccgagctcac gaatcgcctt gtcgcttcga
      541 aatgtcgtgc cggttcgact tcctatcgct gcgatctcgg gtgtcacgtc cggttcgttg
      601 ccggtgatcg ctgcgaccat caccgatgcc cggccgtaga gcttcaagac ccaagcaggc
      661 gccgttcgcg gcgccttcgt gcccgacact cgggcaattt cggacacgaa gtcggctacc
      721 gagcaatcct ctccgcccaa gaggtagcgc tcgccgcttc gacctctggt caccgcgtcg
      781 aggtgcgcgc gaaccacgtc atgaacgtgc gtcaccgaga tgatacccgg tggaatcgcg
      841 gggagggcgc cgtcgcgcag cagagtgaag attcggcccc agctgttgac gtcgtaggga
      901 ccggccacgt cgccgggatt gagaacgacg accttcagtc cgcgcttcgt gccttcgagt
      961 gcttcgagct cgccgagatg cttgcttcgc tcgtagttga tccacgagtc cttgcctcga
     1021 agcggggtct cttcggtgac gatcccctcc actctgcccc aggcggagtt gctcgaggta
     1081 tgcacgaagg tggcgacgcc tttcgccgct gccgcctcga ccatgttccg cgtgccgtcg
     1141 acgttgttcg tcgtttgccg ggcgtttcgc ttcgaccaga aattggtgtc cccagcaacg
     1201 tggaagacga catccgtgcc atcggggatt gcgcgctcga gagattcccg atcgatgatc
     1261 gacccctccg ccaacgccac cgggaagcgc tcgaggtacg tcaggtccga gctcgcgcga
     1321 tgcagggctg tgacctccca gccctcccgg acgagaagct cgaccagatt gataccgatg
     1381 aatcccgtgc caccggtcac gaacgcggtt ttgcccatcg gacccagggc taactcatcg
     1441 agggatcctc gtcgagcgcg ggcacaaccc gagcctcggc tcgcaccccc ccatcgccca
     1501 gttgcggggc caccggcgct cagtgtagac cgatccaacc atgcgctttt cagttttcat
     1561 agtcgttgga gcaatggggt tgcttctctt ggggtccgag ctcgcgaccc aggcccagaa
     1621 cggcaaggtc ccgacgccgg ccgagcttgc tggcagctac acgtacgcag gcgaccgcgc
     1681 gaaagatgaa gctgcgatca cggcgaaggt aaatgccgcc actgcggaga tgagccggat
     1741 ggttctgaaa cgggcaaagc caaagctcgt gtcgagtacc gggattccgg gacagatgga
     1801 catcacgccg caagcggctg atctggtatt caagatggac gattacgtcg tcgcggcccc
     1861 ctcgaacgga agctccgcga aagtaacgac tcctgcgggc gaatccgccg atgcttcctt
     1921 cgactccatg agcgccacgc tcttgcagtc ggtggctaag accggggggg caaaggcgat
     1981 cacgtttgcc ttcaacgagg ccggacagct gctcgcgcac gttcgaatca caaacgaccg
     2041 tttggccggg ccggtcacat acacgctgct ctacgcgcgc tccaagtagc cggagtcgag
     2101 cacctcgatc tgtgcctcga ccacttggcg cattccctcg gacaagacgc gcggctccgt
     2161 gagaagactg acgatgaccc aggcctcctc gggaaaatcg taaaaccagc cgggctgcac
     2221 gagcacaccc cgctcgagaa agcgcagcac ccactcttct tcggagagca cgttgggcaa
     2281 tctcaacact tgataccagc ctccttccgg tgggagcgcc gatgtctccg gcgcgtcacg
     2341 aaggagctcg cgcaggcttc gtcgattgcg ctgcaggcgc tcgaagacga ggcgtcgacg
     2401 cccgtcggat gcgtcgagta gatctggaag tgcacgctgt gtgatctcgt tgaccgaaag
     2461 atacgtgtcc gtgatgagat cgagctgtgt gaggcactct ttgacgagtc gctcctctcc
     2521 cgaaacggcc atccaactca gcttcaactg cggcagcgca gccgccttcg acaagccacc
     2581 cagcgcgaag agcaaaccag agcgggcctc gctgtggcgc agtggctgag gttgtgcttg
     2641 gcgaaggtcg tagggtgcaa agacttcatc gacgacgagc ggcacgccct ggctcgagag
     2701 aaatgcgagg tcctcctcgg aaagatacgc accggtcggg ttgttcgggc tcacggctag
     2761 aatcagcttg acctcaggac ccacaccctc ggcgaaagcc gtgcgatcga tgtgccactg
     2821 cccgtcgtag cgaaacgaat acggtaaagc gacgacgccg tcgagacgtg cgagcattgg
     2881 aaggagggga tagctcggct ccggaatcag aacctggtcc cccgggtcgc agaagagttt
     2941 gatcagagac gaatacgcct cactcgtcgt agcggtgagt accaggtgat cgggattcca
     3001 gtcgagatgc tcacccaacg cttggcgagc cgacgcaagc ccccgcggct gagggtggta
     3061 gagatcgagc gaggcggttt gcatggcccg catggcctcg tctccgaaag cgatgccagc
     3121 gctagtcgga ttcgaatggg tcaaatccca tctgggcggc gtcgcctccg ctgcccgagt
     3181 gatgacattg gggctgaggt cgaacgcgcc ccttctcgag aacatcgcgc gagtatcccc
     3241 gtcgccagac ccaagcgcca ggtcgccttc gaccgtcccg tcttcgtccg gtcatgaaat
     3301 ccacagaaaa ccggatggaa cgaggctggt cgccagcatc acttcgttga tgtcacggtg
     3361 ctccgaccag gttcgggctt cgcgatgctc cggatcgagc cagaacgacc gaatctccga
     3421 tccgaggtag gcgtaaggtg tcagcgcttc gagcccttcg cttgttttcg cgctcagtgc
     3481 caagtcctcc cgcccaactg cctcgtcgat gagctcgatc ccggaaagcc cgatcgcgag
     3541 cccaagctca cgaaaagcca agcgctgcga cgctgggagt ccggaatcgt tctgcctatt
     3601 ccagtacgac aggcccggca gggcggagga aagcagatcg tcgagtagct cacctcgggc
     3661 aaaggccccg agctgcatga gctgcgcgac gcgagacgca tccatcagca gccctcccaa
     3721 acccaatggg tccgccgtgg cccagtcctg gcctcggatc atcttcgcga agtcggccgc
     3781 ctccctttcc aacgtaggtt cactcgccgc ggtcccaagg gccgacctcg tcgtctgaag
     3841 ctgaaccgct gtaatga
//
LOCUS       DASRZF010000020         5277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_358137, whole genome shotgun sequence.
ACCESSION   DASRZF010000020 DASRZF010000000
VERSION     DASRZF010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 5277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5277
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358137"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..548
                     /locus_tag="VFG22_00950"
     CDS             165..548
                     /locus_tag="VFG22_00950"
                     /inference="COORDINATES: protein motif:HMM:NF039194.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5681 domain-containing protein"
                     /protein_id="HET6414831.1"
                     /translation="MDDDRRKLASQADRLKDYRWKPGQSGNPNGQPKRPTFEVVVARI
                     LDEEVPGSDITKREALARVFVAAMLQKNGQMIREYLARSWPVVQKHEVELPGVGTNAL
                     ETALDRFRPNGSAGVSVKPNGNGAA"
     gene            616..1806
                     /locus_tag="VFG22_00955"
     CDS             616..1806
                     /locus_tag="VFG22_00955"
                     /inference="COORDINATES: protein motif:HMM:NF015211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HET6414832.1"
                     /translation="MQWGAGSGKTYTGAQMVRQWTDSGEARTVNIAGPTWVDTMRTMV
                     HGSADAPGLMGVWPEHQRPQIRMAKDDPYLTTHNGAKIQLFAAQKSERFRGAAGGKAW
                     FDEVDAWKPEGMTLEDAFALAEQRIRQGMNPQIICTTTPKRKRLVAQLVKRSDCVVTR
                     ASMYDNESNLPQKYINSQTNRYEGTRLGRQELLGELLPDVAGAIVNAEMIDSQRVVTA
                     PDLIRVVVGVDPFGGGGDACGIGAAAKGIDGEGYVLADRTCRLGPDGWARRAIELAIE
                     VNADCIVYEANFGGDMVPTVLKHAMTAMGASIRVKRVWASKGKAARFEPVGGKYERGE
                     IHHVGTFPELEDEIVQFTPDGYDGEKSPNRADAMVFQLAELFPDLPAVGWDRANELNE
                     RMQA"
     gene            1803..3719
                     /locus_tag="VFG22_00960"
     CDS             1803..3719
                     /locus_tag="VFG22_00960"
                     /inference="COORDINATES: protein motif:HMM:NF016732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HET6414833.1"
                     /translation="MIATAARQKLAAWISPKARADNPIDFGPGYTSKFFGSGLGSNQP
                     DADTLLRESLGVSAMATRAIANRIGSLDPQVKISMRDTSGTLNDEVLDDHILKQLLDR
                     PHPNYSRFQMMWLTANHIVTTGEAYWLKVRNRLRLPMMLQPMPPSKVSPQVEGGVISG
                     YVVQDGKGNSTPLALDDVVRFWFPDPETMYSAEGYLAPNGTVADSQKFSGQHLRSHYQ
                     NDATPPIILQAGAEATDPTDAQLERFQAAWIRKYGNRAGSHRGVPAFLPNGWQAIFAA
                     VASGADVTPLLEHWQSNQLMNFGVPASVLGRVISGDRSAAETNQFVMDLHTITPIAVM
                     ISDALTLQLAPDFDKQLWVEFDEFVSADKRFELEREAQDLTLKIKTVKQVLEQRGDDP
                     EPATWGELPVGTLADTPYTGEERDFGGGFGGFGGGGGESTSDLEEPDDDEPAPDDLPD
                     APRTRSSREMAALHWSKPVSAVRILQREKKFTPVFARALRGVFKKQHEATKKNLSAYR
                     ARTLPSDSELLFDPRGWGELFDLATQAIRERAYLEAASESLNLLGILDEFFFSDAVAQ
                     DLRLQGADMVKLVNQTTAARIRKELKTALAENKTEGETLKQLEKAIDEIFKGRRKNSA
                     TIARTEMHRSSQAG"
     gene            3744..3965
                     /locus_tag="VFG22_00965"
     CDS             3744..3965
                     /locus_tag="VFG22_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414834.1"
                     /translation="MPFKTWLDARDSNVRETHFQEGILPVPREGLFTLPSGHRCNYPG
                     DSMLPAGESINCRCDAAPVYGIGDEVAGG"
     gene            3984..4961
                     /locus_tag="VFG22_00970"
     CDS             3984..4961
                     /locus_tag="VFG22_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414835.1"
                     /translation="MTYERTTAIERGAADAPGRISGILVSDGEASDGHILNIDGAQFP
                     EQAPLLFGHDDSTGTGNLGSWEKFEKVDLPKGRRAIRGVAQIDLDGPKSAQQDWRSDI
                     NYMVDRGHIGQFSVRWDEIGEPARRVNLPSDHPAYVDSAKSQGREAWGYYFDKWKLLE
                     GSVVTLGADPAAMVGRMQQSRGDVRGYWRRAINHALTECSEVAGLVAVSLPSGEFAYI
                     ERAAYDAMLELANERYSLALDLYEDMGTDHFALGAEQRTQEPGKELVPAKEEPSGPIR
                     SALELDAREVVFPNSIEALVAAFSEALEKSEERSRAREDDLFRTATGRI"
     gene            4962..>5277
                     /locus_tag="VFG22_00975"
     CDS             4962..>5277
                     /locus_tag="VFG22_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414836.1"
                     /translation="MQDQDTTTTETTVDEKETATIASLFNAVLEKREKARMASLERQL
                     ALLAATPEDKPEVVEAQRAADNRNERIISRHQPMDSVYRMYHRTNPLYAGVRTPDLDH
                     WNI"
BASE COUNT         1139 a         1650 c         1663 g          825 t
ORIGIN      
        1 tcgcccgagt actgcgacgt gatcctgcaa cgctgggagc ggttcaccga gcgcaaggcg
       61 gagcgcgccg atgcgtagat ggatcgtctg gcacctgccc gaactcgcga ccgcgatgat
      121 cgcgatggcg ctcggattcc ccgtcgtgct aggtgggcga acccgtggat gatgaccgca
      181 ggaaacttgc ctcgcaagcc gatcgactga aggattacag atggaagccg ggacagagcg
      241 ggaacccaaa cggacaaccc aagcgtccaa ccttcgaggt cgtcgtcgcg aggatactcg
      301 acgaggaagt ccccggctcg gacatcacga agcgggaagc actcgctcgc gtgttcgtag
      361 cggcgatgct ccagaagaac gggcagatga tccgcgagta cctcgcgcgg tcgtggcccg
      421 tcgtgcagaa acatgaagtt gagctaccgg gagtcggaac caatgcgctc gagactgcgc
      481 tcgatcgctt ccgacccaac ggctcggcag gagtttctgt caagcctaac ggcaacggcg
      541 cagcatgaac tcgccgcgct ctggagcccg ctcgaccacc aactcgagcc cgagggcgac
      601 tggttcatct gggtcatgca atggggcgcg ggctccggca aaacctacac gggcgcgcag
      661 atggttcgcc agtggaccga cagcggggag gcgcgaaccg tgaatatcgc cgggccgacg
      721 tgggtcgata cgatgcggac gatggttcac ggcagcgcag acgcgccggg gctcatgggc
      781 gtgtggcccg aacaccaacg cccgcagatc cgcatggcga aggacgaccc ctatctcacc
      841 acccacaacg gcgcgaagat ccaactcttc gccgcccaga agtccgaacg gttccgaggc
      901 gcagccggcg ggaaagcttg gttcgatgaa gtcgatgcgt ggaagcctga aggcatgacg
      961 cttgaggacg ccttcgcgct cgcagagcag cgcatccggc agggcatgaa ccctcagatc
     1021 atctgcacca ccacgcccaa gcgaaagcgc ctcgtcgcgc agctcgtcaa acgttccgat
     1081 tgcgttgtaa cgcgcgcttc gatgtacgac aacgaatcca accttccgca gaagtacatc
     1141 aacagccaga cgaaccgata cgagggcacg cggctcgggc gtcaagagct gctcggcgag
     1201 ctgctccccg atgtcgcggg agccatcgtc aacgcggaga tgatcgactc gcagagggtc
     1261 gtcaccgctc ctgatctgat ccgggtcgtc gtcggcgtcg atcccttcgg cgggggcggt
     1321 gacgcctgcg ggataggtgc ggcggcgaaa ggcatagacg gggagggata tgtcctcgcc
     1381 gaccgtactt gcagactggg accggatggg tgggcgcggc gtgcgataga gctcgccatc
     1441 gaggtcaatg ccgattgcat cgtctacgaa gcgaacttcg gcggcgatat ggtgccgacc
     1501 gtcctcaagc acgcgatgac cgcgatgggc gcatccatcc gggtcaagcg cgtgtgggcg
     1561 agcaaaggca aggccgcgcg gttcgagccc gttggcggca agtacgaacg cggggagatt
     1621 caccacgtcg ggacgttccc cgaactcgag gatgagatcg tccagttcac cccggacgga
     1681 tacgacggcg agaagtcgcc caaccgggcg gatgcgatgg tgttccagct cgcagagctg
     1741 ttccccgatc tgcccgctgt gggatgggac cgcgccaacg aactcaacga gaggatgcag
     1801 gcatgatcgc aaccgccgcc cgacagaaac tcgcggcgtg gatctcgccg aaagcgcgag
     1861 ccgacaaccc gatcgacttc ggacccggct acacctctaa gttcttcggc tcgggactcg
     1921 ggagcaacca gcccgatgcc gatacgctcc tgcgcgaaag cctcggcgtg tctgcgatgg
     1981 ccacccgcgc aatcgcgaac cggatcggat cgctcgatcc acaggtcaaa atctcgatgc
     2041 gcgacacgtc gggaacactc aacgacgagg tgctagacga ccacattcta aagcaactcc
     2101 ttgaccgacc gcaccccaat tacagccgct tccagatgat gtggctcacc gcgaaccata
     2161 tcgtgacgac cggagaagcc tactggctca aggtccgaaa ccggctgcgg ctgccgatga
     2221 tgctccaacc gatgccgccg tcgaaggtgt cgccccaagt cgagggcggc gtgatctccg
     2281 gctatgtcgt tcaagacggg aagggcaaca gcaccccgct tgcactcgat gacgtggtcc
     2341 gcttttggtt ccccgacccg gagacgatgt actccgccga aggatacctt gcgcccaacg
     2401 gcacggttgc cgactcgcag aagttctccg ggcagcacct ccggtcgcac taccaaaacg
     2461 atgcgacgcc cccgatcatc ctccaagcgg gagccgaagc aaccgacccg accgacgctc
     2521 agctcgagcg gttccaagcg gcgtggattc gcaagtatgg caaccgcgca ggatcgcacc
     2581 ggggcgtgcc cgcgtttctc ccaaacggtt ggcaagcgat cttcgcagcc gtcgcgtcgg
     2641 gcgcagacgt gacgcccctg ctcgaacatt ggcagagcaa ccaactcatg aacttcggcg
     2701 tgcccgcatc ggtccttggt cgcgtgatct ccggcgaccg atccgcagcc gaaaccaacc
     2761 aattcgtcat ggatctccac acgatcacgc ccatcgcggt catgatctcc gacgcgctca
     2821 cgctgcaact cgcgcccgac ttcgacaaac agctctgggt cgagttcgac gagttcgtga
     2881 gtgccgacaa gcggttcgag ctggagcgcg aggcgcaaga ccttacgctc aagatcaaga
     2941 ccgtcaagca ggttctcgag cagcgcggcg acgatcccga accggcgacg tggggcgagc
     3001 tgccggtggg gacgctcgca gatacgccat acacgggcga agagcgcgac ttcggcggtg
     3061 ggttcggagg cttcggcggc ggtggcggcg agagtacctc ggacctcgaa gagcccgacg
     3121 acgacgagcc cgcaccggac gatctgccgg acgcgccgcg aacccgatcc agccgggaga
     3181 tggctgcgct gcactggagc aagccggtat cggcggtccg aatcctgcaa cgggagaaga
     3241 agttcacgcc cgttttcgcg cgtgcgctgc ggggcgtgtt caagaaacaa cacgaggcca
     3301 cgaagaaaaa cctctccgcg taccgggcgc gcaccctccc gtccgattcc gaactcctgt
     3361 tcgacccgag aggatggggc gagctgttcg acctcgcgac gcaggccata cgggagcggg
     3421 cttacctcga ggccgccagc gaatcgctca accttctcgg catcctcgac gagttcttct
     3481 tttccgatgc cgtggcgcag gatctccggc tacagggcgc ggacatggtg aaactcgtca
     3541 accaaacgac tgccgcccgc atccgaaagg agctgaagac cgcactcgcc gagaacaaga
     3601 cggaaggcga aaccctgaag caactggaga aggcaatcga cgagatcttc aagggccgac
     3661 gcaagaactc cgcgacaatc gcgcggacag agatgcaccg atcctcgcag gcgggatagc
     3721 ttgaggcgtt caaacaggcg caggtgccct tcaagacgtg gctagatgcg cgtgactcga
     3781 atgtaagaga aacgcacttc caagagggga ttcttcccgt tccgcgagag ggtcttttca
     3841 cgcttccaag cggacaccgt tgcaactacc ccggcgactc gatgctccca gcgggcgagt
     3901 cgatcaactg ccgatgtgac gcggctcccg tttacgggat cggcgacgag gtagccggtg
     3961 gataatcgaa cacagggggt cgcatgactt acgaacggac gacggcaatc gagcgcggag
     4021 cggcagacgc tcccggtcgc atctcaggga ttctcgtatc ggacggcgaa gcgtctgacg
     4081 ggcacatcct caacatcgac ggcgcgcagt tcccggagca agcgccgctt ctgttcggac
     4141 acgacgactc taccggcacc gggaacctcg ggagctggga gaagttcgag aaggtcgatc
     4201 taccgaaagg ccgtcgcgcc atccgtggcg tcgcgcagat cgacctcgac gggccgaaaa
     4261 gcgcacaaca ggactggcgt tcggatatca actacatggt ggatcgaggc cacatcgggc
     4321 agttttcggt gcgctgggat gagatcggcg agccggcgcg ccgggtgaat cttccttccg
     4381 atcaccccgc ttatgtggac tccgccaagt cgcagggccg cgaggcgtgg ggatactact
     4441 tcgacaagtg gaaacttctc gaaggctccg tcgtcacgct gggagcggac ccggcggcga
     4501 tggttggccg gatgcaacaa tcacgcggtg acgttcgagg ctactggcgg cgagcgatca
     4561 accacgctct gaccgaatgc agcgaagtcg cgggactcgt cgcggtttcg cttccgtctg
     4621 gggagttcgc ttacatcgag cgcgccgctt acgatgcgat gctcgaactc gcaaacgagc
     4681 ggtactcgct tgcgctcgat ctctacgagg acatgggaac cgatcacttc gcgctcggag
     4741 cagagcagcg aacgcaggaa ccgggaaagg aacttgtacc ggcgaaggaa gagccgtctg
     4801 ggccaattcg gtcggctttg gaactcgatg ctcgggaggt cgtattccca aacagcatcg
     4861 aagcgctcgt cgcggccttt tcagaagcac tggaaaagtc agaagagcga tcccgcgccc
     4921 gtgaggatga tctattccgc acggccacag ggagaatctg aatgcaagac caagacacca
     4981 ccacgaccga aacaaccgtt gacgaaaagg aaacggcgac gatcgcttcg cttttcaatg
     5041 ccgtactcga gaagcgggaa aaggcacgca tggcaagcct cgaacgacag ctcgctttgc
     5101 tcgccgccac accggaggac aagccggaag tcgtcgaagc gcagcgtgca gcggacaacc
     5161 gaaacgagcg gatcatcagt cggcatcaac cgatggattc ggtttacagg atgtatcatc
     5221 ggaccaaccc gctctatgcg ggagtccgca cgcccgacct cgaccactgg aatatac
//
LOCUS       DASRZF010000021        20623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_703708, whole genome shotgun sequence.
ACCESSION   DASRZF010000021 DASRZF010000000
VERSION     DASRZF010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 20623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20623
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703708"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1525
                     /gene="pgi"
                     /locus_tag="VFG22_00980"
     CDS             <1..1525
                     /gene="pgi"
                     /locus_tag="VFG22_00980"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HET6414837.1"
                     /translation="LGELLFDFSKNRITEQTLGFLIELCEECGVPDWIARMFSGEPIN
                     ETEKRAVLHTALRNRGDRPMMVEGADVMPDVRRVLSQMREFVGAVRTGSWLGHSGRSI
                     TDVVNIGIGGSDLGPVMVTEALRPYWKEGLRAHFVSNVDGTHLTEVLRGLQPETTLFI
                     VASKTFTTQETLSNARSARQWLVEQLGAEDAVAKHFVALSTNRKAVAEFGIDPANMFE
                     FWDWVGGRYSLWSAIGLSIATVIGMDNFEALLEGAYEVDEHFRTAPFDENVPVLMAVL
                     GIWYHNFFGAASHAVLPYDQYLHRFPAYLQQADMESNGKSASRDGAIIEQYTTGPIVW
                     GEPGTNGQHAFFQLLHQGTHLVPADFIVAAQSQNPLGQHQDILVANCIAQAEALMRGK
                     TADEARAELEASGMPADQIDFLVPHKTFAGNRPSNTLMLEKLTPRALGRLIALYEHKI
                     FVQGIIWNIYSFDQWGVELGKQLAKVILPELTGETGDHDGSTRQLIDYYRKLKDSDQ"
     gene            1522..2214
                     /locus_tag="VFG22_00985"
     CDS             1522..2214
                     /locus_tag="VFG22_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET6414838.1"
                     /translation="MSTLAVDIGGGSIKALALDDDGVPITEPVARTTPAPATPEAVFR
                     LIRDMAGTLPPHRRVSLGFPGVVKRGVAQNAPNLGNELWHGVSLNQQLGQLLQRPVRA
                     INDADLQGLGVVRGEGVELVLTLGTGMGAALFTEGVLVPNLELGHHPLRDGMTYEDLV
                     RDTELKRLGPARWTARVLEAIGQIEPLFNYDTLHLGGGNVRNLQSTLPANVRVFTLAE
                     ALRGALKLWDDA"
     gene            complement(2211..2852)
                     /locus_tag="VFG22_00990"
     CDS             complement(2211..2852)
                     /locus_tag="VFG22_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6414839.1"
                     /translation="MIIEHFSVAPLGCNCVILGDEERGTAIVVDPGGEAPRILARLHE
                     LGLKCEAIVHTHTHFDHIGATKQVQDATGAPTMIHEEDLFLYEGVQMQLDSFRAPFIA
                     PAVAEIDRFLSDGDTVRAGSLEADILHTPGHSPGSICLTIQGDRPVLIAGDTLFARSV
                     GRSDLWGGDGVQLVASIRRKLFALPDETLVICGHGPNTTIGEERSQNPFVRSA"
     gene            2995..3276
                     /locus_tag="VFG22_00995"
     CDS             2995..3276
                     /locus_tag="VFG22_00995"
                     /inference="COORDINATES: protein motif:HMM:NF018563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HET6414840.1"
                     /translation="MLDVNEYFDGNVKSIGFQTETLRATVGVMAPGEYEFGTSQKETM
                     TVVSGAFTVRLPGSEEWVRFDAGSSFVVDAKRKFQLRVTVDTAYLCTYE"
     gene            3347..4471
                     /locus_tag="VFG22_01000"
     CDS             3347..4471
                     /locus_tag="VFG22_01000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6414841.1"
                     /translation="MRLLFVSHSFPPVGAPLSNMGGMQRVATELHAALEARDDIEIRT
                     LVLRSSWKWVHVKAVPFLISLAARLGREATAQRADTILFTSMTTALPLLIAGPALKKK
                     GIRLAAIVHGLDVTDPNPAYQIAVRRLCGMLDVAMPVSRATGEELVARGLPRDRVQVV
                     PNAVDLERFDGVAASRDQLGSPKIAGAPPLPRDAFLLVTVGRQVRRKGFAWFIENVMP
                     RLPERVVFWLAGDGPERQNIGEAIERTGLRRRVRCLGKISEEDLVELYRRGQLFVMPN
                     IVVPGDMEGFGIVMLEAGACGLPTLAADLEGIGDVIEDGVNGWFAPSEDADAFVQRIE
                     SLLADPEALRAASARTAEYVSSTFRWDRTAERYLEVLRGL"
     gene            5141..5770
                     /locus_tag="VFG22_01005"
     CDS             5141..5770
                     /locus_tag="VFG22_01005"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HET6414842.1"
                     /translation="MQLRSDSFKDGDFLPTRYAFGKTSAEGQIDLSDNRNPHLTWSQL
                     PEGTKSVAVICVDSDVPTKPDDVNQVGRVVPSDLPRTEFSHWVLIDLAPDSSIAEGAF
                     SDGITPKGKEGPRGPGGTRQGVNDYTGWFAGDPDMAGEYYGYDGPCPPFNDSIVHHYR
                     FTAYALDVERLDVDGEFTLSDARAAMDGHVLAEASVAVKYTINPDAKEL"
     gene            5781..6434
                     /gene="nth"
                     /locus_tag="VFG22_01010"
     CDS             5781..6434
                     /gene="nth"
                     /locus_tag="VFG22_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HET6414843.1"
                     /translation="MNRREKADRIGAVLDRLYPEPPIPLVHRDPFTLLVSVMLSAQTT
                     DKKVNEVTPALFAKAPDPASMAALPVDQILAFIRTVGLAPTKAKNLKEMARLLIERHR
                     GVVPDDIDALEALPGVGHKTASVVICQAFGRPAFPVDTHIHRLAARWGLSNGKNVVKT
                     EADLKSLFPEDTWNRRHLQIIYFGREHCPARGHDLGQCEICGWAATKKRIAEESARR"
     gene            6481..7503
                     /locus_tag="VFG22_01015"
     CDS             6481..7503
                     /locus_tag="VFG22_01015"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET6414844.1"
                     /translation="MSIQFRLFRIPIRIHLWFWLMALWLWTLRSDEGWPGLLIWVLVV
                     FQGILMHELGHALVGRAFGRQPRIELVALGGLTWWEQREPMSPGRSLLVSAAGPVVGI
                     FIGSFAFVLMDVLQVPDPSLARYAFRSVIWVNLGWGLLNLLPVLPLDGGNIVASIMEF
                     VAPARGRLLACYVSFGVIGLLFAVTVTFKQYPMTILLLLLGFSTYQAFRMERQRLDPT
                     PEQGAPPPSLLNQAFEALERGDGQALVRVASELVAKAQSTEELDEAFHLLAWGRLING
                     EPGEAQQALTSMSGERSADPALEGAVLVELGKPADAVPFLEEACERGGDFALNYYQRA
                     LREKTA"
     gene            7575..8567
                     /locus_tag="VFG22_01020"
     CDS             7575..8567
                     /locus_tag="VFG22_01020"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET6414845.1"
                     /translation="MPPSAAEAPRWELYRLLGEPRRLRLLGLTSEDELSIGELAELTR
                     EAQPNVSKHLKLLREAGLVAVRRQGTRAFARLSPDLNGDPVLADAIRSGRALCAEDGS
                     LERVPSVIQSRDDEARAFFEQAANESALRPVAEWSAYLTAVRSLLPHRSLAVDLGTGD
                     GALVELLAPLFERVIAVDRSEEQLKAAERRLVSRGYDHVELLQAGYDDPELATRVREL
                     GGADVVFAVRLLHHAPKPQQVVSLLGSLVRPGGQVVVIDYCSHDDELMRATQADLWLG
                     FERDELIDFATSAGLVNPEVFLIPAARYGPGPDAHLKWQVLAAQREPKEETRQS"
     gene            8564..9892
                     /gene="ahcY"
                     /locus_tag="VFG22_01025"
     CDS             8564..9892
                     /gene="ahcY"
                     /locus_tag="VFG22_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HET6414846.1"
                     /translation="MSPNPELKHKVADLSLAAWGRKEIAIAEHEMPGLMALRREYGAQ
                     KPLAGARIAGCLHMTIQTAVLIETLRELGAEVTWTSCNIYSTQDHAAAAIAATGVPVF
                     AWKGETEEEYDWCIHQQIRAFEGGKAPNLLLDDGGDLTAIVHKQYPELFEGPDAIIGL
                     SEETTTGVHRLYDMERDKSLKCPAINVNDSVTKSKFDNLYGCRESLVDGIKRATDVMI
                     AGKVVVVAGYGDVGKGCVQAVARAGARVLVTEIDPICALQAAMEGFQVVTMDEAAPIA
                     DIFVTATGCCDVVTGEHMKQMKNEAILCNIGHFDSEIQMSWLTDASNGVTEEEIKPQV
                     DHFIFPDGKRIIVLARGRLVNLGCATGHPSFVMSNSFSNQVIAQLSLWTERDRYQPGQ
                     VYVLSKELDEKVAHLHLGQLGVHLTKLTATQAEYLGVPAQGPFKPSYYRY"
     gene            9899..10801
                     /locus_tag="VFG22_01030"
     CDS             9899..10801
                     /locus_tag="VFG22_01030"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET6414847.1"
                     /translation="MVSLLIEAWRYFASHDGRLLSGAIAFYASLAIAPLGVVALLIAS
                     LVMPEAEARAELVGQVGSFIGSDLATFLVEAIVQLGDKTTSWAAPVLGLLFMLYISTR
                     LFSMLRRALNHMWGIRPKLLVHSSRSEMFHFAQRRLLAFAMVLIFGSSLLVLVLLHSA
                     AAAAARVFGDGGGTVTLVEFGSAFAVLAPLIALVYRWLPDAIISWRDVWVGAAVTCLF
                     VLAGSFLIGIYLGYASPASIYGAAGSVIVLLLWIYYTTQIFLMGAVFTRTWARRHGRA
                     IEPLSYAEVVETRDYSASVSASET"
     gene            complement(10768..11811)
                     /locus_tag="VFG22_01035"
     CDS             complement(10768..11811)
                     /locus_tag="VFG22_01035"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6414848.1"
                     /translation="MARARESLMPFVRNGVDKAANRLSRLGYGVLPWLIQSWRQVHVE
                     HNVPYRNTGRRSHLLDIYRRKDALGLLPTIVYVHGGAFSMMSKDTHRIMAYVLAAQGY
                     QVFNINYRLGPVHTYPKPLKDAMAALEWVLDNGAKYGADTKRLSIIGESAGANLTSAL
                     AYCATHPRPEPFAKRIFDRNMKISCVAPLYGLLDMHDVRRFWLDPDKNRRMPGWIKGE
                     IKGTAYSYLGSRVKRALQFPLASPLRLFEQPACPGSRPLPPFFTTVGTADPLLADTIR
                     LSDALDRRNTECELHVFRGEIHAFNVLLWRAAAREQWGALFDFLETHMHGTVEEAPKW
                     APHYVSLAETLAE"
     gene            complement(11840..13381)
                     /locus_tag="VFG22_01040"
     CDS             complement(11840..13381)
                     /locus_tag="VFG22_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET6414849.1"
                     /translation="MAIVEPLSPTSEGRRRLGIRSPATRESVGEIVVSSPDDVALAIA
                     KARDAQEAWARVPIAERARIVRGAIDVLVEKRAEVAKTIIDETGKTKLESLIMEVLPG
                     CDYINFWCGRALKELSDEKRKLHGYLRPYKKLLMHYRPLGVVGIITPWNGPFSLAINP
                     SVQAILAGNAVILKPSEVAPYSGDWAVKLLREAGVPEGVVQTVHGDGETGAALVNGGV
                     QKISFTGSVQTGKKIAAACAAQLIPCTLELGGKDAMIVCEDADIERAAGGAVFNSMLN
                     SGHVCMGVERVYVVESVADEFEERVEKIVRELRYGTGDDVDVGSVFWDRQLPIIERHI
                     ADAKAKGAEIVVGGEADTTRGIFFKPTFVRKLDHSMDLMREETFGPIVSVMRVADEEE
                     AIRLANDSQYGLSGSVWTRNVQKGIEIAKRLETGSVVINDASMAYGAPEAPFGGMKDS
                     GVGQVNGLGGLRGYTHPQPILIDRWAAKKERVWYPHTPKSVEEIDGMIRFIYGSALKK
                     LGFFS"
     gene            13509..16193
                     /locus_tag="VFG22_01045"
     CDS             13509..16193
                     /locus_tag="VFG22_01045"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HET6414850.1"
                     /translation="MRGWSWATLLLFVVVGCGNASPPGPPRLGAGGIDFSCDCPFGEE
                     CASDGLCVSICGEVAACVFGGTAQCCGAGTACQAGSCVTDCGQAVDCKGLCCDEGQVC
                     LGEACVARCEDPARLCGADDELCCAIDEACIGGACVPLRNPCSFGEDCELDELCEPSL
                     GVCIPRDAVEVCEFRPPVGNFAPDIACRWTPPAAPMGASSQELEIARMSEVVMTPSVA
                     NLTDDNGDGVTDLRDIPDVVFVSFDYAQDGCCTRRGVVRVVSGACNVDGTMTTHATLK
                     GLSSDDWIGNSTGIALGNLHPDDLSEERVPEIVATFKNGGTIAWRRTASDGSAWEVMW
                     QNDDLPSNQHTRGGAQPSIADLNADGRPEIIIGNVVLDGLTGKGPNDPDLPANALAWD
                     GRELEATVPGANLGIGNNAFLGPVSTVADLDRDGLQEVIAGNTVYNYDGSERWTYEYT
                     TTNSSCGGSLDCDGYNAVGNFDDDEEAEVVIIRLGELFILNHDGSPVPGIPLPIRIPG
                     APGDVAAPTYSPSSYVPTEPLFNADGDLLPPERILCGGALQLLAYDAAGDPVMSESSQ
                     VSVRTAGANESGPPTVADFDGDGFAEVGTASSTAYVVFDFQCTGNPLPPDCDREWVRW
                     MVENDDCSSRATGSSVFDFEGDGSAEVVYADETTFRIFRGSDGAILYEDTTHSSNTRV
                     EMPIVVDVDNDGKSEIVIPEPNTDPSRGGIEIWEDADNNWVRTRRIWNQHAYSVTNVT
                     EDGQIPRVPETNWTRSRLNNFRQNVQPGGLFDAPDFVVREIFRIDCSDTEFELALVLG
                     NDGSLSVPPGILTHVWVTTNDDRVFDLGTVATRDWLLPGQSEQLEFLFEIPTGPEVTA
                     IVVTASADDDGMDGQQYNECDEENNASRSNPISCPSVQ"
     gene            16275..16799
                     /locus_tag="VFG22_01050"
     CDS             16275..16799
                     /locus_tag="VFG22_01050"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6414851.1"
                     /translation="MLRTSTVPPASRLLIRQGDLSDLSAVVRICETHAEYWDGRLPSE
                     ILLHQFFDRALRSQDDIFQFWVAENGHGTMIGWEMLTAASADPGNRRLTAEATTCLGQ
                     QSASLGRTLATHAIRHASRTPLQYVFARVPTRDTLMVGVLEAVGYQEVGLIPEAQKPP
                     AHPEQLEFVYVVER"
     gene            17168..17332
                     /locus_tag="VFG22_01055"
     CDS             17168..17332
                     /locus_tag="VFG22_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414852.1"
                     /translation="MTERPPSYLEGVPRDHAELVSGLLQVVRGAAKDLKTLITQAIAM
                     SNQKNDRFDR"
     gene            17329..18534
                     /locus_tag="VFG22_01060"
     CDS             17329..18534
                     /locus_tag="VFG22_01060"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6414853.1"
                     /translation="MSAISRIAVVGASLAGLRAVEFVRRANFAGELVLIGDEPHLPYD
                     RPPLSKQLLRGEWDQERLALRRKNYDELNAELILGQRALSLDTAAREVCLEGGHTVGF
                     DGLVIATGAEVRKLPRQPKLDGIFALRTLEDALAIRDALASQPRVAVVGAGFIGAEVA
                     ASARQLGLEVTLIEALETPLAESLGTRLGGRLQSIHERHGVRVLSGRRVDGFGGSDRV
                     TSLHLDDGGEVPCDLVVVGIGVSPSVSWLEGSGLELDDGVRCDATLATSVPGIVAAGD
                     VASWHNPLFDERMRVEHWTNAVEQARHAVSTLLAAPGEARPFESVPIFWSDQFDIKIQ
                     GVGRPKPSDDLIVVEGAADGEKLVALYGRAGRLVGAVTFNQAPKLVQLRMLIAKRGGL
                     DEAMKIAES"
     gene            18538..19440
                     /locus_tag="VFG22_01065"
     CDS             18538..19440
                     /locus_tag="VFG22_01065"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6414854.1"
                     /translation="MSIRETDVAFESGDDACKGWFMLPELADASLPCIVMAHGFGLTR
                     RCSLRELAVAFVGAGYAVLLFDYRGFGDSGGQPRQLVSFRRQQEDWNAAIDFARAQRE
                     VNSSRIATWGFSLGAGHALSVAADDDAVAAVVAVAPMFDGLSSTLAAARRWSPLNFLR
                     IVGRALRDFVGAGFGRPPVTIRIAGNPGELALLTSPDAYPGYRAIVPSDFNFDIAARI
                     GLLFWTYWPGLRLRQFSRPILVLPSGIDKINPPGPTMRRARRCESASIVELDCEHMEV
                     VIEPHRSFVIRATLEFLGRSLPSR"
     gene            complement(19432..20157)
                     /locus_tag="VFG22_01070"
     CDS             complement(19432..20157)
                     /locus_tag="VFG22_01070"
                     /inference="COORDINATES: protein motif:HMM:NF037435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HET6414855.1"
                     /translation="MGDSRRHRGPHPADEEIFGEDALPRLRDAVQDLSWLRSRGYAEA
                     SALKLVGDRYQLRRRQRVAVGRCACSDAAKARRASKLCEPGAVMGQRLLIDGLNVITT
                     IEVALAGGVLLLGRDGCMRDMASFHGSYRIVHESERAVAILVDVVEAMKPSEARVYID
                     RPVSNSGRLAELVRRTAAARGSCLEATTADRVDERLQASTAVVASADSAILERCGPWL
                     NLARIAVEPLKPELDLFWLLDLS"
     gene            20334..>20623
                     /locus_tag="VFG22_01075"
     CDS             20334..>20623
                     /locus_tag="VFG22_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414856.1"
                     /translation="MNPVSPTILAVTDLTSASYKLVGFAAFIAGSSDGEVILVSTYGG
                     GGRSGPSEPPPAWAGNGDSWRDCIELRLRQEGQLLERQRQWCSDRGVRCRAEL"
BASE COUNT         3723 a         6415 c         6526 g         3959 t
ORIGIN      
        1 actcggcgag ctgctattcg acttttccaa gaatcgcatc accgagcaga ccctcggctt
       61 cctaatcgag ctgtgcgagg agtgcggcgt cccggattgg atcgcgcgga tgttctccgg
      121 cgaaccgatc aacgaaaccg aaaagagggc ggtccttcac acggccctgc gcaatcgtgg
      181 cgatcgccca atgatggtcg aaggcgcgga tgtgatgccc gacgttcggc gtgtgctctc
      241 acagatgaga gagttcgttg gcgccgtccg aacgggaagc tggctcggac acagcgggcg
      301 aagcatcacc gatgtcgtca acattggaat cggaggctcg gatctcggcc cagtgatggt
      361 gaccgaggca ctgcgtcctt attggaaaga gggacttcgc gcgcacttcg tgtccaacgt
      421 cgatggaaca catttgaccg aggtcctgcg tgggcttcag cccgaaacca cgctcttcat
      481 cgtcgcctcc aagaccttca ccacacagga gacactcagc aacgcgcgct ccgcgcggca
      541 atggctcgtc gagcaactgg gtgccgagga tgcggtcgcg aaacacttcg ttgcgctgtc
      601 tacgaaccgc aaggccgtgg cggagttcgg aatcgacccc gcgaacatgt tcgagttctg
      661 ggactgggtc ggcgggcgct actcactctg gtcggccatt ggcctctcga tcgcgaccgt
      721 catcggcatg gacaacttcg aggccttgtt ggaaggcgcc tacgaggtag atgagcactt
      781 ccggaccgcc cctttcgacg agaacgtgcc tgtgctcatg gccgttctgg gcatctggta
      841 ccacaatttc tttggcgccg cgtcccatgc ggtgctgccg tacgaccagt acctccaccg
      901 cttcccggcg tatctccagc aggccgacat ggagagcaat ggcaagagcg catctcgtga
      961 tggcgcgatc atcgagcagt atacgacagg ccccatcgtc tggggggagc ccggaaccaa
     1021 cggccagcac gctttcttcc aactcttgca ccaaggaaca cacttggttc cggcggactt
     1081 catcgtagcg gcccaaagcc agaacccact cggccagcac caggatatcc tcgtcgccaa
     1141 ctgcatcgct caagccgagg ccttgatgcg cggaaagacg gcggacgaag ctcgggcgga
     1201 gctcgaagcc agcggtatgc ccgcggacca gatcgacttc ctcgtgccgc acaagacctt
     1261 cgccggcaat cgaccgagca ataccttgat gctcgagaaa ctcaccccgc gagcgctcgg
     1321 ccgtttgatc gcgctctacg aacacaagat cttcgtccag ggcatcatct ggaacatcta
     1381 cagcttcgat cagtggggcg tagagctcgg taagcagctc gccaaagtga tcctcccgga
     1441 gctgacgggt gagaccggcg accacgacgg ctccacgcgc cagctgatcg actactatcg
     1501 caagctgaag gattcagacc aatgagcacg ctggcggtcg atattggtgg tggtagcatc
     1561 aaggcgctcg cgctcgacga tgacggggtg cccatcaccg aaccggttgc tcggacgaca
     1621 ccggcacctg caacccccga ggccgtgttc aggttgatcc gcgacatggc tggcaccctg
     1681 ccaccgcatc ggcgtgtctc gctcggcttc ccgggtgtcg tcaagcgcgg cgtcgcgcag
     1741 aacgcaccca atctcggaaa tgagctctgg cacggggtgt ctctgaatca gcagctcggc
     1801 cagctgctcc aacgccccgt gcgggccatc aacgatgccg acctccaggg tctaggggtc
     1861 gtgcgtggag agggcgtcga gctcgtgctg accttgggca ccggcatggg tgcagcgctc
     1921 tttacggagg gagtattggt tccgaatctc gagcttggtc accatccact gcgcgacggg
     1981 atgacatacg aggatttggt ccgcgacacc gagctcaagc gcctcggccc tgctcgatgg
     2041 accgcgcgcg tcctcgaagc catcgggcag atcgagcctc tcttcaacta cgacacgctc
     2101 cacttgggcg gtggtaacgt tcgcaacctg cagtcgactc tccctgccaa cgtgcgtgtg
     2161 ttcacgctcg cggaggcgct ccgcggcgcg ctcaagctct gggacgacgc ctaggcactg
     2221 cgcacgaacg ggttctggct gcgctcttca ccgatggtgg tgttcgggcc atgtccgcag
     2281 atcacgagag tctcgtcggg aagcgcaaag agcttgcgcc gaatcgaagc gaccagctgc
     2341 acaccgtctc caccccagag gtcggagcgg ccgacgctgc gcgcgaagag agtatcccct
     2401 gcgatgagta ctggccgatc gccttgtatc gtgaggcaga tgcttcctgg tgagtgtccg
     2461 ggcgtatgca ggatgtcggc ctcgagggag ccggcccgca ccgtgtcgcc atccgagagg
     2521 aagcggtcga tctcggcaac cgcgggtgcg atgaatggtg ctcgaaagct atcgagctgc
     2581 atctgcacgc cttcatagag gaacaagtcc tcttcgtgga tcatcgtcgg cgcgccggtg
     2641 gcgtcctgca cctgcttggt ggcaccaatg tgatcgaaat gcgtgtgcgt atgcacgatc
     2701 gcttcgcact tcaatccgag ctcgtggagg cgcgccagaa ttcgcggcgc ttcaccaccg
     2761 gggtcgacca cgatggccgt gccgcgctcc tcgtcgccca ggatcacgca gttgcagccg
     2821 aggggcgcta ccgagaagtg ttcgatgatc atggcgcctt tctagcaccg ctcaccctgg
     2881 ctgacaccag caggcgcccg tgttatctcg acgttgaacg ttttcggccg cgccccgcgc
     2941 caacccatgg gggcacgccg ataccgtcga gaagatccgt cgcggaggat gagcatgctg
     3001 gacgtcaacg agtactttga tgggaacgtg aagtcgattg gatttcaaac cgagacgctt
     3061 cgcgcgaccg tgggtgtcat ggcccccggc gagtacgagt tcggtacgag tcaaaaagag
     3121 accatgacag tcgtgagcgg cgcatttacc gtcagactac ccggctccga agagtgggta
     3181 cgcttcgatg cgggctcgag cttcgtggtc gacgccaagc ggaagttcca gcttcgggtg
     3241 accgtcgata ccgcgtatct ctgtacctac gagtagcgcg gcgctggaag tcccggcgcg
     3301 ctcagtccta gcgcgcttcc agctcctcct gtatcaagca tccctgatgc gtcttctctt
     3361 cgtctcgcac tcgtttcccc ccgtgggtgc tccgttgagc aacatgggtg ggatgcagag
     3421 ggtggccacc gagctccacg ctgctctcga ggcgcgtgac gacatcgaga tccggacgct
     3481 cgttctacgg tcgagctgga aatgggtcca tgtcaaagcg gtaccgtttc tgatctcgct
     3541 tgcggctcgg ctcggtcgcg aggcgacagc ccagcgggcg gacacgatct tgttcacgag
     3601 catgaccacg gctcttccgc tgctgattgc gggacctgcg ttgaaaaaga aggggatacg
     3661 cctcgccgca atcgtgcatg gactcgatgt gaccgatccg aatccggcgt accagatcgc
     3721 ggtgcggcgc ctctgcggta tgctcgatgt cgccatgccg gtgagtcgcg cgactggcga
     3781 agagctggtc gcgcgtggtc taccgcgcga tcgggttcag gtcgttccga acgccgtcga
     3841 cctcgaacgc tttgatggag ttgctgcgtc acgcgaccag cttggttctc ccaagatcgc
     3901 gggggccccg ccgcttccga gggatgcctt cctgctggtg accgtgggtc gccaagtccg
     3961 gcgaaagggg ttcgcgtggt tcatcgagaa cgtgatgccg agactacccg agcgcgtggt
     4021 cttctggttg gctggagatg gccccgagcg ccagaacatc ggggaggcga tcgaacgcac
     4081 cggcctacgg cgccgcgttc gctgcctcgg aaaaatctcg gaagaggatc tcgtcgagct
     4141 gtatcgacga ggccagctct tcgtgatgcc caacatcgtg gttccgggcg acatggaggg
     4201 tttcggtatc gtgatgctcg aagccggagc ctgcggactg cccacgctgg cggcagactt
     4261 ggaaggcatt ggcgacgtga tcgaggacgg cgtcaacggc tggtttgcac ccagtgagga
     4321 cgctgacgct ttcgtccagc ggatcgaatc cttgttggcg gatcccgaag ccctccgcgc
     4381 ggcaagcgcg cgcacggccg agtacgtcag ctcgacattt cgctgggacc gcacagccga
     4441 acgctacctc gaagtccttc gcgggctctg atcgggcact ggcatgaacg gggcgcgggc
     4501 gaacacgaca cggggttccg gtcgaacggc tcggcttgca attgtggtcg gatagactgg
     4561 cggggtttga ccacgcaagc ctcgcctcga cgctcgcgcc aatcggaggt ggttgctgtc
     4621 tgaccgattg tcactcgcga cgtcgccgga accccgcatc gcgttcgccc gcgcccctcc
     4681 gcgactcagt gtcagtgaga gagacagaag cagcggatgg ttcacactga cgctggcact
     4741 gacgacgtgg ctcaggtggg agcggacgga gggggacggc tcgggatggg cagcgcagcg
     4801 attgggtcga gcctagccgt aggtcgtctt agggaactgg gggcgagtgg gtcccaatcg
     4861 cgagcacgcc cgtcgcgagc cgttccccgg ttgccggcac tgacgctggc gctgacgctg
     4921 acgctggcgc tgagatacgg aggaggacgg ctcgggatgg gcagcggagc gattgggtcg
     4981 agcctagccg taggtcgtct tagggaattg ggggcgagtg ggtcccaatc gcgagcacgc
     5041 ccgtcgcgag ccgtcctccg gtgacccagt cgcgcacacc gttgctggca ctgacgctga
     5101 ccaattgaca gtcgctggtg ccggtgctga tctctcccgc atgcagcttc gcagtgatag
     5161 tttcaaagac ggtgactttc tgccgacccg gtacgccttc ggcaagacca gcgcagaggg
     5221 tcagatcgac ctttccgaca acaggaatcc tcatctgacc tggtcgcaat tgcccgaggg
     5281 cacgaagtcg gtcgcggtta tttgcgtcga cagcgacgtg ccaaccaagc cagatgacgt
     5341 gaatcaagtt ggccgcgtcg tgccatccga tctaccgcgc acggagttca gccactgggt
     5401 cttgatcgat ctggcacccg attccagcat cgccgagggc gcgttttccg acgggatcac
     5461 gcctaaaggc aaggaagggc ctcggggtcc cgggggcacg cggcagggcg tgaacgatta
     5521 taccggctgg ttcgcgggcg atccagacat ggccggcgag tactacggct acgacggacc
     5581 ctgtccgccc ttcaacgata gcatcgtgca ccactaccga ttcaccgctt acgcgctcga
     5641 cgtcgagcgg ctcgatgtcg atggtgagtt cacgctcagc gacgcgcgcg ccgcgatgga
     5701 cggccacgtg cttgcagaag cgagtgtcgc cgtgaagtac acgatcaatc ccgacgcgaa
     5761 agagctctag tgcgcagccg atgaatcgtc gggaaaaagc cgaccgcatc ggggcggttc
     5821 tcgacaggct ctaccccgag cctcccatac cgctcgttca ccgcgatccc ttcacgctct
     5881 tggtgtcggt gatgctcagc gcgcaaacca ccgacaagaa ggtcaacgag gtgaccccgg
     5941 ctttgttcgc aaaggcgcct gatcccgcat cgatggccgc tctgccggtg gatcagattc
     6001 tcgcgttcat tcgcaccgtc ggactcgctc caaccaaggc caagaatcta aaagagatgg
     6061 cgcgcttgct gatcgaacgg caccgagggg tcgtccccga cgacatcgat gcgctcgagg
     6121 cgctcccggg agtcggtcac aagaccgcgt cggtggtgat ctgccaggcg ttcggccgcc
     6181 ctgcctttcc cgtcgacacg catattcacc ggctcgccgc gcgctggggt ctctccaatg
     6241 gcaagaacgt cgtcaaaacg gaggccgacc tcaagagctt gtttcccgag gacacctgga
     6301 atcgccgtca cctccaaatc atctacttcg gtcgtgagca ctgcccggct cgcggtcacg
     6361 atctcggcca gtgcgagatc tgcggctggg cggcgacgaa gaagcgaatc gccgaggaat
     6421 ccgctcgacg atgagcctcg gcactggtcg cgcgggcagg gccgacccat attgagcgca
     6481 atgagcattc agttcaggct tttccgaatc cccatccgca ttcacctctg gttctggctc
     6541 atggcgctct ggctatggac actccgaagc gacgagggat ggcccggctt gctcatctgg
     6601 gtgctcgtgg tctttcaggg cattctgatg cacgagctcg gccatgcatt ggtcggacgc
     6661 gcgtttggcc gacaacctcg aatcgagctg gtcgcgctcg gcggcctgac ttggtgggag
     6721 cagcgagagc cgatgagccc tgggcgaagt ctcctcgtca gcgcagcggg tcccgtcgtc
     6781 ggtatcttca tcggctcttt cgccttcgtg ctgatggacg tgctccaagt tcccgatcca
     6841 tcgcttgccc gctacgcgtt tcgctcggtg atttgggtca atctcggttg ggggctcctg
     6901 aatctcctgc cggttctccc gctcgacggc ggcaacatcg tggcctcgat catggagttc
     6961 gtcgctccag cgcgagggcg tttgctcgcc tgctacgtgt cgttcggcgt catcggtttg
     7021 ctcttcgcag tcactgtcac cttcaagcag taccccatga cgattctctt gctcttgttg
     7081 ggcttcagca cctatcaggc gtttcgcatg gaacggcagc gactcgatcc gacgccggag
     7141 cagggcgcgc caccgccgag cctcctcaat caagctttcg aagcgctcga gcggggggat
     7201 ggccaagctc tagtccgcgt ggcgtccgag ctggtcgcca aggcgcagtc gaccgaggag
     7261 ctcgacgagg cgttccatct tctagcctgg ggccggctga tcaatggcga gcctggagag
     7321 gcacagcagg ccctcacctc gatgtcgggt gagcgcagtg cagaccccgc gctcgaaggc
     7381 gccgtgctgg tggagcttgg aaagcccgcc gacgcagtgc ccttcttgga ggaggcttgt
     7441 gagcggggcg gagatttcgc gctgaactac tatcagcggg cattgcgcga aaaaacagcg
     7501 taattacaga aagttgaaag aattgtcggc cgctgagctt gcatccatgc gaatgggggc
     7561 atatatcaaa ggccgtgcct ccgtccgccg ccgaagcgcc tcgctgggag ctctaccgtc
     7621 tactcggaga gccgcggagg ctccgcttgc tcgggctcac ctccgaggac gagctctcga
     7681 tcggcgagct ggcggagctg acgcgcgagg cccagcccaa cgtctcgaag cacttgaagc
     7741 tgcttcgcga ggccgggctg gtggccgtgc gacggcaggg gacgcgcgcc ttcgcgcggc
     7801 tttctccgga cctcaacggc gaccctgttc tggcggacgc gattcgttca ggaagggcac
     7861 tctgcgccga ggacggcagc ctcgagcgcg ttccttcggt catccaatcc cgtgatgacg
     7921 aagcgcgcgc cttcttcgag caagctgcga acgagtccgc cctgcggccc gtggcagagt
     7981 ggtccgccta tctcaccgca gtgcggagct tgttgcctca ccgcagcctc gccgtcgacc
     8041 tcggcaccgg ggatggtgcc ctggtggagt tgctcgcgcc gctgttcgaa cgtgtcatcg
     8101 ccgtcgaccg ctcggaagag cagctcaagg cggccgaacg taggctggtg agccgtggct
     8161 acgatcacgt cgagcttctc caagccggct acgatgatcc ggagcttgca acccgcgtgc
     8221 gagagctcgg aggcgccgac gtcgtttttg ccgttcgctt gctccaccat gcgccgaaac
     8281 cccagcaagt ggtttcgctg ctcgggtcgc tggtgcgacc gggcggacag gtcgtggtca
     8341 tcgactactg ttcccacgat gacgagctca tgcgcgcgac gcaggccgac ctttggctcg
     8401 gcttcgaacg cgacgagctc atcgatttcg ccacttcagc cgggttggtc aaccccgaag
     8461 tatttctaat tcccgcggcg cgctacgggc ctggccccga tgcccatttg aagtggcagg
     8521 tgcttgcagc gcaacgcgaa cccaaagagg agacaagaca atcatgagcc cgaatccaga
     8581 actgaagcac aaggtggccg acctttcact ggccgcatgg ggtcgaaaag aaatcgcgat
     8641 cgccgagcat gagatgcccg gcttgatggc gcttcgaaga gaatacggcg cccagaagcc
     8701 gctcgccggt gcgcgcatcg ccgggtgcct ccacatgacg atccagacgg cggtcctcat
     8761 cgagaccctg cgcgagctcg gtgccgaggt cacctggacg agttgcaaca tctactcgac
     8821 tcaggaccac gccgcggctg ccattgccgc caccggcgtc cccgtttttg cgtggaaggg
     8881 tgagaccgag gaggagtacg actggtgtat ccaccaacag atccgtgcct tcgaaggcgg
     8941 aaaggccccc aacctgcttc tcgatgacgg cggtgacctc accgcgatcg tgcacaagca
     9001 gtatcccgag ctcttcgagg gaccggacgc aatcatcggt ctctccgagg agaccaccac
     9061 cggcgttcac cggctctacg acatggagcg cgacaagtcg ctcaagtgtc ccgcgatcaa
     9121 tgtgaacgac tccgtcacca agtcgaagtt cgacaaccta tatggctgcc gcgagtccct
     9181 ggtcgatggc atcaagcgcg ccacggacgt gatgatcgct ggcaaggtcg tcgtcgtcgc
     9241 tggctacggc gacgtcggca agggttgtgt gcaggcggta gcgcgtgccg gcgctcgtgt
     9301 tctcgtcacc gagatcgatc caatctgcgc gcttcaggcg gcgatggaag gttttcaagt
     9361 cgtcacgatg gacgaggcgg ctccaatcgc cgacatcttc gtgacagcca ccggctgttg
     9421 cgacgtggtc accggcgagc acatgaagca gatgaagaat gaggccatcc tctgcaacat
     9481 cggacacttc gattcggaga ttcagatgtc ctggctgacg gacgcttcga atggggtcac
     9541 cgaagaagag atcaagccgc aggtcgacca cttcatcttt cccgatggca agcggatcat
     9601 cgtgctcgca cgtggccgcc tggtgaacct cggctgcgcc acgggccacc cgagcttcgt
     9661 catgagcaac tccttcagca accaggtgat tgcgcagctt tcgctctgga ccgagcgcga
     9721 tcgttaccaa ccaggtcagg tctacgtgct ttccaaggag ctcgacgaaa aggttgcaca
     9781 cctgcacctt ggtcagctgg gcgtgcatct caccaagctc acggctaccc aggccgaata
     9841 cctcggcgtt ccagctcagg gtcccttcaa gccgagctac tatcgctact gatcgcgtat
     9901 ggtctcgctg ctgatcgagg cttggcgcta cttcgcgtcg catgatgggc ggttgctctc
     9961 cggagcaatc gccttctatg cgtcgctggc cattgcgccg ctcggcgtgg tcgcactgct
    10021 catcgcatcg ctggtcatgc cggaggcgga agctcgagcg gagctcgtcg gtcaggtcgg
    10081 gtccttcatc ggctctgact tggcgacctt cctcgtcgag gcgatcgtgc agctcggcga
    10141 caagaccacg agctgggcag ctcccgtcct cggactcctt ttcatgctgt acatctcgac
    10201 gcgcttgttc tcgatgctac ggcgcgcgct caatcacatg tggggcattc gtccgaagct
    10261 tctggttcac tccagccgct ccgagatgtt tcacttcgcc cagcggaggc tcctggcttt
    10321 cgcaatggtc ttgatcttcg ggagctcact gctggtcctc gtgttgctgc actccgctgc
    10381 tgccgcggcc gcgcgtgtct tcggagacgg cggaggcacg gtcactctcg tcgagtttgg
    10441 cagcgcattc gcggtcctgg cgccgttgat cgccctcgtc taccgctggc ttcccgacgc
    10501 gatcatctcc tggcgggacg tctgggtcgg agcggcggtc acctgcctgt tcgtgctcgc
    10561 cgggtccttt ctcatcggga tctacctcgg atatgccagc cccgcgtcca tctatggggc
    10621 ggccggttcg gtgatcgtgc ttctcctttg gatctactac accacgcaga tcttcctcat
    10681 gggtgcggtg ttcacgcgga cctgggcccg ccggcacggc agagccatcg agccgctttc
    10741 ttacgcggag gtcgtcgaga cgcgcgatta ctcggcaagc gtttccgcaa gcgagacgta
    10801 gtgcggcgcc catttgggcg cttcctcgac ggtgccgtgc atgtgcgtct cgaggaaatc
    10861 gaaaagagca ccccactgct ctcgtgcagc cgcgcgccag agcaagacat tgaaggcgtg
    10921 gatctcgccg cgaaagacgt gtagctcgca ttcggtattt cgccggtcga gcgcgtcgct
    10981 cagtcgaatc gtgtccgcca agagggggtc ggccgtgcct accgtggtga agaacggcgg
    11041 aagaggccgg cttccaggac aggctggctg ctcgaataga cgcaaggggc tcgcgagcgg
    11101 aaactgaagc gctcgtttga ctcggcttcc cagatacgaa tacgcggtgc ccttgatctc
    11161 gcctttgatc cagccgggca tgcggcggtt cttgtcgggg tcgagccaaa agcgtctcac
    11221 gtcgtgcata tcgagtagac cgtagagagg cgcgacgcag gagatcttca tgttgcgatc
    11281 gaagatccgc tttgcaaaag gctccggtcg aggatgcgtg gcgcagtacg cgagcgcaga
    11341 tgtgaggttt gcacccgccg actcgccgat gatcgagagg cgcttcgtgt cggcaccata
    11401 cttggcccca ttgtcgagca cccactcgag ggcagccatc gcgtccttca ggggcttcgg
    11461 ataggtgtgc acggggccga gccggtagtt gatgttgaac acctggtagc cctgtgccgc
    11521 gaggacgtag gccatgattc gatgcgtgtc cttcgacatc atcgaaaagg cccctccgtg
    11581 aacgtagaca atcgtgggga gaagcccgag cgcgtccttc cgacggtaga tgtccaagag
    11641 gtgagagcgt ctgccggtat tccgatacgg aacgttgtgc tcgacgtgta cctggcgcca
    11701 gctttggatc agccagggga gcactccgta gcccagccgc gaaagacggt tcgctgcttt
    11761 gtccacgccg ttccgcacga acggcatgag cgattcacgg gcacgggcca tagcgattca
    11821 gtataagcga tgcgctgagc tacgaaaaga agccgagctt cttgagggcg cttccgtaga
    11881 tgaaccgaat cattccgtcg atttcttcga cggatttggg ggtatgcgga taccagacgc
    11941 gctccttctt cgctgcccag cgatcgatca ggatgggctg cgggtgggtg tacccgcgaa
    12001 ggcccccgag accgttcacc tggccgaccc cgctgtcttt catcccaccg aaaggtgcct
    12061 ctggcgcgcc gtatgccatc gatgcgtcgt tgattacgac agagcctgtc tcgaggcgct
    12121 ttgcgatctc gattcccttc tggacgttcc tggtccaaac acttccgctc aatccgtact
    12181 gtgagtcgtt ggcgagacga atcgcctctt cctcatcggc cacgcgcatc accgagacga
    12241 taggtccgaa cgtttcctcc cgcatcaagt ccatgctgtg gtcgagcttg cgcacgaacg
    12301 taggcttgaa gaagatcccg cgcgtcgtgt cggcttcgcc accgaccaca atctcagcgc
    12361 ccttggcttt ggcgtccgct atgtggcgct cgatgatcgg aagctgccgg tcccagaaca
    12421 cagatcctac gtccacgtcg tctccggtgc cgtatcggag ctctcttacg atcttttcca
    12481 cgcgctcttc gaactcgtcg gcgacgcttt cgaccacata gacccgctcc acacccatgc
    12541 aaacatggcc tgaattgagc atcgagttga agacagcacc acctgctgcg cgctcgatgt
    12601 cggcgtcttc acagacgatc atggcgtcct tgccgccgag ctccagcgtg caggggatca
    12661 actgcgcggc acaagcagcc gcgatcttct tgccggtttg aacacttcct gtgaacgaga
    12721 tcttctgcac accaccgttc accagagccg ccccggtttc gccgtcgcca tgcaccgttt
    12781 gcacgacgcc ttccgggacc cccgcttctc tcagcaactt caccgcccag tctccggagt
    12841 agggcgcgac ctcggacggc ttcaggatga ctgcgttgcc agcgaggatg gcttgcacgc
    12901 tgggattgat ggcgagcgag aacggaccgt tccaaggggt gatgatccca acgacgccga
    12961 gcggccggta gtgcatcagc agcttcttat atgggcgcag gtaaccatga agctttcgct
    13021 tctcgtccga gagctccttg agcgcgcggc cacaccagaa gttgatgtaa tcgcagcctg
    13081 ggagaacttc catgatcagc gactcgagct tggtcttgcc ggtctcgtcg atgatcgtct
    13141 tcgccacctc cgcgcgcttc tcgaccagga cgtcgattgc gccccgaacg attcgggcac
    13201 gctctgcgat cgggacacgc gcccaggcct cctgagcgtc ccgcgccttg gcaatcgcca
    13261 gagccacgtc atcggggctg ctgacgacga tctcgcccac tgactcgcga gtggccgggc
    13321 tccgaattcc gagccggcgg cgtccctcag aggtcgggga aagtggttcg acgatggcca
    13381 tccgttcgct cctcgtgcag ggaggtccca gggaccccga atgaaacacg ttctactgac
    13441 tcgtctggca aacgcaagca gcctcgactt ggtggcatag gtgaaatatt acacttatat
    13501 ttcaaatcat gcgtggatgg tcctgggcga cgctgctctt gttcgtcgtg gtcggttgcg
    13561 gaaatgcgtc tcctcccggg ccgcctcgtc tcggagcggg cggaatcgac ttttcctgtg
    13621 actgtccgtt cggtgaggag tgcgcctcag acgggctctg cgtctcgatc tgtggtgagg
    13681 tcgcggcctg tgtgtttggc gggaccgctc agtgctgtgg cgcggggact gcatgtcagg
    13741 ctgggagctg cgtcaccgat tgcggccaag ccgtcgactg caaaggcctc tgctgtgacg
    13801 agggtcaggt ctgcctcggg gaagcctgtg tggctcgatg cgaggacccg gcgcgcttgt
    13861 gcggtgcgga cgacgagctc tgctgtgcga tcgacgaagc ctgtatcgga ggcgcttgcg
    13921 tcccgctccg gaacccttgt tcctttggcg aagactgcga gctcgacgag ctctgtgaac
    13981 ctagcctggg cgtgtgcatt ccacgggacg cggtcgaggt ctgcgagttc cgcccgccgg
    14041 tcggcaactt tgcgcccgat atcgcatgca ggtggactcc gcctgcggcg ccgatgggcg
    14101 cgagcagcca ggagctcgag atcgctcgaa tgagcgaggt cgtgatgacg ccctccgttg
    14161 cgaatctgac ggacgacaac ggcgacggcg tgaccgacct gcgtgacatt cccgacgtgg
    14221 ttttcgtgag cttcgactac gcgcaggatg gctgttgtac gagaaggggt gtcgtgcgcg
    14281 tcgtttcggg cgcttgcaac gtcgacggaa ccatgaccac gcacgctacg ctcaagggcc
    14341 tttcttccga tgattggata ggcaattcca cgggaatcgc gcttggcaat ctccacccag
    14401 acgatctgag cgaagaacgg gttccggaaa tcgtcgcgac gttcaagaac ggaggaacca
    14461 tcgcctggag acgcaccgcg agtgacggat ccgcttggga ggtcatgtgg cagaacgatg
    14521 atcttccgag caaccaacac acgcgcggcg gcgcgcagcc gtcgattgcc gacttgaatg
    14581 ccgacgggag acccgaaatc atcatcggca acgtcgtcct cgatggtctc accggcaagg
    14641 ggccgaacga ccccgacttg ccggcgaatg ctctagcctg ggacggacga gagctcgagg
    14701 cgacggtgcc cggagcgaat ctcggcattg gcaacaacgc gttcctcggc ccggtctcga
    14761 cggtggcgga cctcgaccgc gacggtctcc aagaggtcat cgccgggaac accgtctaca
    14821 actacgacgg cagcgagcgc tggacgtacg aatacaccac gacgaactcg agttgcgggg
    14881 gttcgctcga ctgcgatggc tacaatgcgg tcggcaattt cgacgatgac gaagaggctg
    14941 aggtcgtgat cattcgactc ggtgagctct tcattctcaa tcacgatggt tcgcccgtac
    15001 ccggcattcc gcttcccatt cgaatcccgg gtgctcccgg cgacgtggcc gcgccaacgt
    15061 attcgccgtc ttcgtacgtg cccacggagc ctctcttcaa cgccgacggc gacctcttgc
    15121 ctcccgagcg aattctctgc ggcggagcct tgcagcttct ggcctatgat gcggccggcg
    15181 acccggtcat gagcgaaagc tctcaagtca gtgttcgtac cgccggcgcg aacgagagcg
    15241 ggccaccgac ggttgccgac ttcgatggcg acggattcgc ggaagtaggc acggcgagct
    15301 cgaccgccta tgtggtgttc gactttcagt gcacgggaaa ccctctgcct ccggattgcg
    15361 accgcgagtg ggtccgctgg atggtggaga acgatgactg ctccagccgc gcaaccggat
    15421 cgagcgtctt cgacttcgaa ggtgacggtt ctgccgaggt ggtctacgcg gacgaaacga
    15481 cgttcaggat ctttcgaggt tcggacggtg cgattctcta tgaggacacg acccatagca
    15541 gcaacacacg cgttgagatg ccgatcgtcg tcgacgtcga caatgacggg aaatcggaga
    15601 tcgtgatccc agagccgaat acggatccaa gccggggggg aatcgagatt tgggaagacg
    15661 ccgacaacaa ctgggtgcgc acgcggcgaa tttggaatca gcatgcgtac agcgttacga
    15721 acgtgaccga ggacggacag attcctcgag tccccgagac caactggacg cgttctcgac
    15781 tgaacaactt ccggcagaac gtccagccgg gcggactgtt cgatgcgccg gatttcgtgg
    15841 tgcgagagat cttcaggatt gattgcagcg acacggagtt cgagttggcg ttggtgctgg
    15901 gcaacgatgg ctctttgagc gtgcctccgg ggatcctcac ccacgtttgg gtcacgacca
    15961 acgacgacag ggtcttcgat ctcggcaccg tggctactcg cgactggttg cttcccgggc
    16021 aatccgagca actcgagttc ctgttcgaga ttcccactgg gcccgaggtt acggcgatcg
    16081 tggttaccgc aagcgcggac gacgacggga tggatgggca gcagtacaac gagtgcgacg
    16141 aagagaacaa cgcgtctcgc tcgaatccga tcagctgccc ctcggttcaa taagccgcag
    16201 aatcgcaact taatcgcggc ctccaaacga gggattgcgg atctcgagcg tggcgggata
    16261 taccagttaa tccggtgctt cgtacctcga cggttccgcc ggctagccgg cttctcattc
    16321 gccagggaga tctctcagac ctgagcgcgg tggttcggat ctgcgagacg cacgcggagt
    16381 actgggacgg tcggcttccg agcgagatcc tgctccacca gttcttcgac cgcgcgctgc
    16441 gttcgcaaga cgatatattt cagttctggg tcgccgagaa cggccatgga acgatgattg
    16501 gctgggagat gctcacggct gccagcgccg atccagggaa ccgaaggttg acggccgagg
    16561 ccaccacctg ccttggacag caaagcgcta gtctgggacg cacactggcg acccatgcga
    16621 ttcggcatgc gagccgaacc ccgcttcagt acgtgttcgc gcgggtgccc acacgggaca
    16681 cactcatggt cggtgtgctc gaggcagtcg gttaccaaga agtcggtttg attccggaag
    16741 cgcagaagcc gcccgcgcat ccggagcagc tcgagttcgt ctacgtggtc gaacgctagc
    16801 gagtcgggac gcacttgctc tccgcgaggt ttccgataag gtgcctgaac ccggaggcgc
    16861 aatggcgacg aagaaaacgc ccgaaaaaac gactcggacc gcaaagaagg cacctaggaa
    16921 ggcgcccaaa aaggcaccaa agaagcgcac gagcaaggct cccagaaaag cgacggcgaa
    16981 gaaacgtccg tcgaggagca gtgggcctcc aaagacgggg agcaaggcgc cttcgaggcc
    17041 gaaccgtccg acgattccgg gaggacccgt ggcgaagaag gctgcccagg tctcagcgac
    17101 ggcggcagtg tcagcaccga ggggcgcccc agtgcccctg gcgagctcag cgccaagccc
    17161 aaaaccagtg acggagcgac ccccttcgta tctcgagggc gttcctcgtg accatgccga
    17221 gttggtgtct ggcctgctcc aggtcgttcg cggcgccgcg aaggatctca agacactcat
    17281 cacgcaagcg atcgcgatga gcaaccagaa gaacgaccgc ttcgatcgat gagcgcgatc
    17341 tcgcggattg ccgtcgttgg ggcgtctctc gccggccttc gcgcggtcga gttcgttcgg
    17401 cgcgcaaact tcgccggcga gctcgtcctg atcggcgatg agccccatct tccgtacgac
    17461 cgaccgccgc tttccaagca gttgcttcga ggtgaatggg accaggaacg gctcgcgctc
    17521 cgcaggaaga actacgacga actgaacgcg gagctaattc tcggccagcg tgcgctttcg
    17581 ctcgacaccg cagcgcgtga ggtctgcctc gagggcgggc acacggtcgg cttcgatggg
    17641 ctggtcatcg cgacgggcgc cgaggttcgc aagcttcccc ggcaaccgaa gctcgatggc
    17701 atcttcgcgc ttcgaacctt ggaagacgct cttgcaattc gcgatgctct cgcgagccag
    17761 cctcgcgttg cggtcgttgg tgcaggcttc atcggcgcgg aagtggccgc ctcggcccgg
    17821 cagctcgggc tcgaggtgac cctgatcgaa gccttggaga ctcccctcgc ggagagcctg
    17881 ggcacgcgtc tcggaggcag gctccagtcc atacacgagc gtcacggcgt gcgggttctc
    17941 tccggccggc gcgtcgacgg cttcggcggc tccgaccgag tcaccagcct gcacctggat
    18001 gacggcggcg aggtcccttg cgatctcgtg gtcgttggaa tcggagtcag tccatcggtg
    18061 tcctggctcg agggttctgg cctcgagctc gacgacggag tccgatgcga tgcgacgctc
    18121 gccaccagcg tccccgggat cgtcgccgcc ggtgacgtcg ccagttggca caacccattg
    18181 ttcgacgagc gcatgcgcgt cgagcactgg accaacgcgg tggagcaggc gcgccacgcg
    18241 gtgtccacct tgctggcggc gccgggtgag gcaaggccgt tcgagtcagt gccgatattc
    18301 tggtcggatc aattcgacat caagatacaa ggagtcgggc ggcccaagcc gagcgacgat
    18361 ctcatcgtcg tggagggtgc tgcagacggc gagaagctgg tcgccctcta cggtcgcgct
    18421 gggcgactgg tcggtgccgt gacattcaat caggctccca agctcgttca gcttcgcatg
    18481 ctcatcgcca agcggggtgg acttgatgag gcgatgaaga tcgcggagtc ttagcgcttg
    18541 agcattcgag aaaccgacgt ggcgttcgag agcggcgacg atgcctgcaa gggctggttc
    18601 atgctacccg agctggcgga cgcttcgttg ccttgtatcg tcatggcaca cgggttcggc
    18661 ctgacccggc gttgcagcct tcgtgagctt gctgtcgcat tcgtgggtgc gggctatgcc
    18721 gttcttctgt tcgactaccg gggcttcgga gatagtggag ggcaaccgcg acagctcgtt
    18781 tcgttccgca ggcagcaaga ggactggaat gccgccatcg atttcgcgcg agcgcagcgc
    18841 gaggtgaatt cgagccgaat tgcgacttgg ggcttttcgc taggcgccgg acacgcgctc
    18901 agtgttgcag ccgacgacga cgcggtggcg gcggtcgttg cagtggcgcc catgttcgat
    18961 ggcctcagca gtactctggc tgcggctcgt cggtggagtc cactcaactt cctgcggatc
    19021 gtgggacgag cgctccggga tttcgtagga gccggcttcg gccgtccccc ggtcacgatt
    19081 cgaatcgctg gaaacccagg cgagctcgcg ctactcacct cgcccgacgc gtatcccggg
    19141 tacagggcca tcgtgccgag cgacttcaac ttcgatatcg cggcgcgaat cggcctgctt
    19201 ttctggacct attggcctgg ccttcgtttg cgacagttca gtcgcccgat cctggtgctt
    19261 ccctcgggaa tcgacaagat caatccgccc ggtcccacca tgcgcagagc tcggcgctgc
    19321 gagtcggcca gcatcgtcga gctcgactgt gagcacatgg aagtcgtcat cgagccacac
    19381 cggagcttcg tcattcgcgc cacgctcgag ttcctcggcc gttcgctgcc ttcacgatag
    19441 atccagaagc cagaacaagt cgagctcggg tttcagcggt tcgactgcga tgcgggccag
    19501 gttcagccag gggccgcaac gctcgagtat cgcgctgtca gcgctggcga ccaccgcagt
    19561 ggaagcttgc aggcgctcgt ccacgcggtc ggctgtcgtc gcttcgaggc aggagcctcg
    19621 cgccgcagcg gtccttcgaa cgagctccgc gaggcggccg gagttcgaaa ctggccggtc
    19681 gatgtagacc ctggcttccg agggcttcat cgcttccacg acgtcgacca agatcgccac
    19741 ggcacgctcg ctttcatgca cgatccgata gcttccgtgg aagctcgcca tgtcgcgcat
    19801 gcacccgtcc cgtccgagca ggagaacccc gccggcgagc gcaacctcga tcgtggtgat
    19861 cacgttgaga ccgtcgatca gcaggcgctg acccatcacc gcaccgggct cgcagagctt
    19921 cgatgctcga cgagctttcg cggcatctga gcaagcgcag cgcccaacgg caacgcgctg
    19981 ccgcctccga agctgatatc ggtctcccac gagcttgaga gccgatgcct ctgcatagcc
    20041 gcgactgcgg agccagctca agtcctgaac cgcgtctcgc aagcggggaa gcgcatcctc
    20101 gccgaatatt tcctcatcgg cgggatgcgg cccgcgatgc cgtctggaat cacccatgcc
    20161 gaatcctagc cgttccgagc agcccatcgc gccttcccaa tccgggcttc ggcgcagaaa
    20221 atgcgtatcg ctagattctc ccgcaagcct tgctgcgggt cgagggacga agaggcattt
    20281 cgccgggctt tcttgactgg cacgtgattt gctctgttgg ttttcaagct tcgatgaatc
    20341 ccgtgtcacc caccattctc gccgtcacgg acctgacctc tgcgagctac aagctcgtgg
    20401 ggttcgccgc gttcatcgcg gggtcgtcgg atggtgaggt cattctggtc tcgacgtacg
    20461 gcgggggggg ccgcagcggt ccatcggagc ctccgccggc ttgggctggc aacggcgata
    20521 gctggcgtga ctgcatcgag ctccggctgc gacaagaggg gcagctcctc gagcgccagc
    20581 ggcaatggtg ttccgaccgt ggggttcgtt gcagagcaga gct
//
LOCUS       DASRZF010000022        11020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1736697, whole genome shotgun sequence.
ACCESSION   DASRZF010000022 DASRZF010000000
VERSION     DASRZF010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 11020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11020
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1736697"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..126)
                     /locus_tag="VFG22_01080"
     CDS             complement(<1..126)
                     /locus_tag="VFG22_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013983884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HET6414857.1"
                     /translation="MADTDFYAVLGVSRSSSPEEIKKAYRQLAKKLHPDRNPDNKA"
     gene            318..1094
                     /gene="uppS"
                     /locus_tag="VFG22_01085"
     CDS             318..1094
                     /gene="uppS"
                     /locus_tag="VFG22_01085"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HET6414858.1"
                     /translation="MALVDVSRLPKHVAIIMDGNGRWAQARGQARTAGHERGADAVRA
                     TMRAARRLGIPALTLYAFSEQNWDRPEGEINALVDLLCECLVSERNELVDNEIRLSAI
                     GRLHKLPKHAQSRLSALTSETQSNHGMTMTLALSYGGREELVDATRELARRASAGEIK
                     PNEIDESLIDELLPSMEVGPVDLLIRTGGEKRISNFLLWGSAYAELYFSDELWPDFDA
                     NSLYLAIHDYQARERRFGLVPEAESATACSKAGKELERAV"
     gene            1091..1936
                     /locus_tag="VFG22_01090"
     CDS             1091..1936
                     /locus_tag="VFG22_01090"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HET6414859.1"
                     /translation="MNEAIEQPPQKKRLSGTALRVATVVPLIPIIMWMMFAGPRWLWQ
                     AFILLAIAIGAYELMSMKVPSSRGLSIWGSLTSVVFAYSIIAVDTMPAMYGALLLIVL
                     GAMTWSLLQDDPLHDASVRVGWLLGTPVYVGGCMASLALVRDFNPTGAWVFLTMALAW
                     GSDTAAYFVGRKFGKTKLAPRISPKKTLEGSAGGLAASVLGALAMSFFLPGLSPMDAI
                     ALGILAGAAGQAGDLLVSVLKRSSGVKDSGGILPGHGGILDRVDALAFTAPATWAYGH
                     FIAGF"
     gene            complement(2024..2851)
                     /locus_tag="VFG22_01095"
     CDS             complement(2024..2851)
                     /locus_tag="VFG22_01095"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HET6414860.1"
                     /translation="MNLKDKWLPVFAMTLFTGGLSGCTQVDAGNLGVVTKWGEIQDIA
                     LQEGIHFRTPLKTQIINISTRVHKMEASASASSKDLQVVSTKIALNYRIDAAQVVEVF
                     RNVGTRVTVESTIIDPALQESLKKATAQYTAEELITKRQEVKETLTESIRATLAKNDL
                     LVTELSITDFQFDPQYQQAVEAKQVAEQRALTARNDLARIKVEAEQEEAKARGVANAM
                     LARAEAEAKAQELLKKTVSPEIVHLRAVEKWDGKQPMVVGGSGMILDFGAISKAAER"
     gene            complement(2903..3556)
                     /locus_tag="VFG22_01100"
     CDS             complement(2903..3556)
                     /locus_tag="VFG22_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414861.1"
                     /translation="MSTTSLFASLAIGAISLGSLHSLAPDHWLPIAAVARARNWPRAR
                     AMRVAFICGFGHVTVSVLLGLLALMFGAQMFQSLGERMVSVAGLLLIGFGVAYAIWGL
                     RGAFAHRLHGHHHHHYDHVHDASGASTWSLFLIYCADPCVAVIPIMFAAAPLTTAETV
                     TIVVAYEIATVGAMVVLVGLAHSGAQLFKGKWIERYGDITAGALIVATGVMVAILDW"
     gene            complement(3543..4277)
                     /gene="hypB"
                     /locus_tag="VFG22_01105"
     CDS             complement(3543..4277)
                     /gene="hypB"
                     /locus_tag="VFG22_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HET6414862.1"
                     /translation="MCETCGCGDPEIVPVEVQERILADNDTQAAHNRSHFEGLGLLAI
                     NLMGSPGAGKTALLEATAKAAGKDLKLGAVSGDLATERDADRLNASGIVSATITTGSA
                     CHLDARLVHHALHHFPSSDRDIFFIENVGNLVCPAIYDLGQAANVVALSVTEGEDKPL
                     KYPVMFQRADLVLLTKIDLIPHLDFDVSAVEDALARVMPNAAMLKVSAKTGEGIDAWL
                     TWLAEHRPSPVEGRTMQKHEHSHEHH"
     gene            complement(4270..4617)
                     /gene="hypA"
                     /locus_tag="VFG22_01110"
     CDS             complement(4270..4617)
                     /gene="hypA"
                     /locus_tag="VFG22_01110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HET6414863.1"
                     /translation="MHEYSIVASLIDRVQQEAEARGSSRVHRLHVKIGELAGVELDLL
                     KTAFDTFRERTICDGAELLIHAVSATWACPSCKRTVERGAILRCDTCGRPARMVQGDE
                     IILERIEMEAPDV"
     gene            complement(4621..5454)
                     /locus_tag="VFG22_01115"
     CDS             complement(4621..5454)
                     /locus_tag="VFG22_01115"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET6414864.1"
                     /translation="MKHIRVERSGAVGILTIDRRERLNSLDVETAQDFRRAGLQLARD
                     RDVRAVLVRAEGGVFCSGADLKYIRDGGDDADLGYLQPGTRQPNEGYGEIFKQILEYI
                     HSTISEIRRAPKPFIAVVDGPAAAGGFGIAMSCDLVLASDRAWFEWAYFKTGLTGAES
                     STFFLPRLLGLRKALELVLLNPRLSAVEALDWGLINRVFPHESLDSEALRIATQLADA
                     PTAAVGVAKNLLNQAAGMDRLDVHLDAELTELARIADGANFAEGISAFFEKRAPEFES
                     E"
     gene            complement(5467..6507)
                     /gene="hypE"
                     /locus_tag="VFG22_01120"
     CDS             complement(5467..6507)
                     /gene="hypE"
                     /locus_tag="VFG22_01120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HET6414865.1"
                     /translation="MTRNARFIDERITLKHGTGGGAMRALIEQVFAKGLGDVGPDGIG
                     LAAMDDGAAIRIGDQYLVIATDSHVVQPIEFPGGDIGRLAVCGTVNDLAMMGATEPLA
                     LTCAAIIEDGFPRDTLKRIWESIRSACEEAGTTIVTGDTKVMGHGELDGLVLNTTGVA
                     LTKRLVSDAGLRPGDKILVTGTMGDHGLAIMATRNELALETSLESDVAPVNTLVRHAL
                     DVGGNGVVALKDPTRGGLSSALHEMAEKSGVGIIIDETSVPLRDDVRSVGELLGIDPL
                     VSANEGKAVFGLRPEQAEAVLDAIRSHPLGRNAAIIGECVAERPGFVILDTGFGSRLL
                     AEAEGELLPRIC"
     gene            complement(6504..7652)
                     /gene="hypD"
                     /locus_tag="VFG22_01125"
     CDS             complement(6504..7652)
                     /gene="hypD"
                     /locus_tag="VFG22_01125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HET6414866.1"
                     /translation="MNPSEAFAQLKFRDPARARGLRDALERLVQGADHAPVSVMHVCG
                     SHEQAIAKFGLRAAFPQELQVIMGPGCPVCVTDVPEVDEAVALAKQGVRIASYGDMLR
                     VPGTSQSLADAKAEGAKVDVVYSVTQAVDLAREHDEDIVFFASGFETTAVATAAVLLD
                     DPPDNFSVLSAHKYIPPVMEIVAEMPGAKIEGFLAAGHAATITGSAVFEKFVERHGLP
                     VVIAGFEPLDVLAGLVKLLEVINSGEPRVENAFPRCVTREGNVQAQKMLWRVFRPTGG
                     RWRGIAHVPNGNLRLRDEFAKFDTRKRFTIDASALWSYAPPKLVQQCQCGDIMAGIAS
                     PSDCQLFGKECTPDTPVGACMVSAEGTCKIWHQYGGRPDLRSIKRGAA"
     gene            complement(7639..7962)
                     /locus_tag="VFG22_01130"
     CDS             complement(7639..7962)
                     /locus_tag="VFG22_01130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HET6414867.1"
                     /translation="MCLGVPGKVIEVDREMQIATVDFWGVKKQVRLEVCDAPVAPGDY
                     ILNHVGFAIRRIPAEEAAETLALYDELLRLADDQDLMTADIRAEIEAAPDATPTRDGA
                     TDESK"
     gene            complement(7982..10261)
                     /gene="hypF"
                     /locus_tag="VFG22_01135"
     CDS             complement(7982..10261)
                     /gene="hypF"
                     /locus_tag="VFG22_01135"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HET6414868.1"
                     /translation="MNTGRRIEIRGTVQGVGFRPWVYRMARRSGVVGRVRNDARGVTI
                     EAFAPEEILERFVQELKSNPPPSARIVELRAAPIPVQDLDGFAIESSGASGDKALSIP
                     PDLTTCVDCRAEVMDPEDRRFGYAFTNCTVCGPRFTIATGIPYDRSATTMRTFPMCDA
                     CRREYDDIEDRRFHAQPNACPQCGPSLDLVRSDGTPCSTDNPLAEAAELLMSGSIVAV
                     KGLGGFHLSCDAWNEAAVLRLRQRKHRDEKPFAVMVADLDAAHAIAEIDPEEEALLSS
                     AERPIVLVRARADSSLAAAVNPDTPLLGLFLPYTPLHHLLLAAAGRPLVMTSANVAEE
                     PICRDNDEAQDRLEGIADAVLLHDRDIAMRCDDSVVRVVAGVPTILRRSRGFVPRPFR
                     LAKPVSRPVLACGGHLKNTFCIATGDSAYFGQHIGDLESLAALEFFEESIDRMETILS
                     VRPEVVAHDLHPGYISTRYAGTRTGATRVGVQHHHAHVASLIAESGIEGPVLGVAYDG
                     TGFGEDGTSWGGEFLLVFRGRYERLASFRPLRLAGGNVAMREVWRIAYALVHDAFDGA
                     PPIEALAVFEGIAPEHLRIVSQMLERDLNSPKARGLGRYFDGFGALGLCRPQSAYEGQ
                     VAIAWDQAANANVRDAYPFDIDRSATPIEVDLRAAVRAAVADLLSGVAAGDVSARFHN
                     TVVTVTVAVVEALLAECGAHPVALTGGVFQNARIAGGIEEKLAHKNRVLRHREVPPGD
                     GGIALGQALIADAILGDKG"
     gene            complement(10258..10656)
                     /locus_tag="VFG22_01140"
     CDS             complement(10258..10656)
                     /locus_tag="VFG22_01140"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET6414869.1"
                     /translation="MKLMGALLTSLALLACSARDDSSPSDYSEVQVPIERLASDDARA
                     RGRALYRKKCALCHGERADGNGVRSKGLSGKPINFQNKEWLASTSPLALFEVLSEGKR
                     GTSMPAWPTLGDEEKWDLIAYVLSVAEDGP"
     gene            complement(10607..>11020)
                     /locus_tag="VFG22_01145"
     CDS             complement(10607..>11020)
                     /locus_tag="VFG22_01145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HET6414870.1"
                     /translation="GTLGLSLLPLVDSADQVILVDAIRADGPPGTQVRIEGDEVAPAV
                     YERLSPHQIGVADLLAGASLLDRYPDRVLVLGVVPESIELGLERTPAVQASIPELVQR
                     VVEELTAMGYPPVVRSENRDEAYGSAAHVARTLGV"
BASE COUNT         2076 a         3524 c         3295 g         2125 t
ORIGIN      
        1 cgccttgttg tcagggttcc gatcaggatg cagctttttg gcgagttgcc ggtaggcctt
       61 cttgatctcc tcagggctcg agctccggga aacccccaag acagcgtaga aatcggtgtc
      121 agccatgacc actcaaccta accaccccgg atacgctttc aacggcgatt tcatcgcgcg
      181 gagctgcttg agcgactgcg tcgaggccaa gtgcgacaat tttgtctctt atgaatatat
      241 tttgtgacaa gcttgtccct tcatgctctg agatagccgt tcttgctgcc tttggacctg
      301 catgatactc gctcgccatg gccttggtcg atgttagccg cctgcctaag catgtcgcga
      361 tcattatgga tggcaatggg cgatgggctc aggctcgggg tcaggcacgc acagccggac
      421 acgagcgcgg tgccgatgcc gttcgcgcaa cgatgcgcgc cgctcgtcgc ctcggtattc
      481 cggcgctcac cttgtacgcg ttcagcgagc agaactggga tcgtccagag ggggagatca
      541 acgcgctcgt cgatctgctt tgcgagtgcc tcgtatcgga acgcaacgag ctcgtcgaca
      601 acgagattcg tttgtctgcg atcggccgac ttcacaagct tcccaaacat gcacagtcgc
      661 ggttgagcgc gctcacatcc gagacgcaat caaatcacgg aatgacaatg acgctggcgc
      721 tgtcgtacgg cggacgcgag gagctcgtcg acgcaacgcg cgagttggcg agaagagcca
      781 gtgcaggtga aatcaaaccc aacgagatcg atgaatcatt aatcgacgag ttgctgccat
      841 ccatggaagt cggccctgtg gatctactga ttcggaccgg tggtgagaag aggatcagca
      901 acttcctcct ctgggggtcc gcctatgccg agctttactt cagcgatgag ctttggccgg
      961 atttcgacgc gaattcgctc tatctagcga tccatgacta ccaggcgaga gaacgccgat
     1021 ttggtctggt tccggaggcg gaatccgcta ccgcctgctc caaagcaggc aaagagttgg
     1081 agcgtgcggt atgaacgaag cgatcgagca acctcctcag aagaagcggc tctcgggaac
     1141 ggcgcttcgc gttgccactg tcgtcccgct gattccaatc atcatgtgga tgatgttcgc
     1201 gggtccgcgc tggctctggc aggcgttcat cctgttggcg atcgcgatcg gcgcatacga
     1261 gctgatgtcg atgaaggttc cttcttcacg cgggctcagc atctggggct cactcacctc
     1321 agtcgtgttt gcgtactcga tcatcgccgt cgacacgatg cccgcgatgt acggagcgct
     1381 gctgctgatc gttctcggtg caatgacctg gagcctcttg caggacgacc cacttcacga
     1441 cgcaagcgtc cgcgttggct ggctcttggg gacccccgtg tacgtcgggg gatgcatggc
     1501 gtcgcttgcg ctagtccgcg atttcaaccc caccggggca tgggtgttct tgacgatggc
     1561 gctcgcctgg ggcagcgaca ccgcggcgta cttcgtcgga cggaagttcg gcaaaacgaa
     1621 gctggcacct cgcatctcgc cgaagaaaac tttggaaggc tccgcgggcg ggctcgcggc
     1681 ctcggtactc ggcgccctag ccatgagctt cttcctgccc ggcctcagtc cgatggacgc
     1741 gatcgcgctc ggcattcttg cgggcgcggc cggacaagcg ggcgacctct tggtgtccgt
     1801 gctcaagcga agcagcggcg tgaaggatag tggcgggatc ctccccgggc atggcggcat
     1861 tctcgatcga gtcgatgcac tcgccttcac cgcgcctgcg acctgggcct atgggcactt
     1921 catcgccggc ttctgataga cccggccccg tgctggtcga gctcgtcggg ctgaccagcc
     1981 gggctcgtca cgccttcgtt ggcggcaacg ggccgggcgc tgatcagcgt tcggcagcct
     2041 tgctgatggc cccgaaatcg aggatcattc cactgcctcc gacgaccatc ggctgcttcc
     2101 catcccactt ctcaacagca cgcaagtgca cgatttcagg gctaactgtc ttcttgagga
     2161 gctcctgggc cttggcttct gcctcggctc gcgcgagcat cgcgttggcg acaccccgcg
     2221 ccttggcttc ttcttgctcc gcttcgacct tgatgcgtgc gagatcgttc ctcgcagtca
     2281 aagcccgctg ctctgccacc tgcttcgctt cgaccgcttg ctggtactga gggtcgaatt
     2341 ggaagtcggt gatcgagagc tccgtcacga gaaggtcgtt cttcgccaga gtcgcgcgaa
     2401 tgctctcggt gagtgtttct ttcacctctt gacgcttcgt gatcagctcc tccgcggtgt
     2461 actgcgccgt cgctttcttg agagactctt ggagagcggg atcgatgatg gtgctttcta
     2521 ccgtcacccg agtcccgaca tttcgaaaga cctcgacgac ttgcgctgca tcgattcggt
     2581 agttcaaagc gatcttcgtc gacacgactt gaaggtcttt cgaagacgcg gatgcgctcg
     2641 cctccatctt gtgcacgcgc gtcgagatgt tgatgatctg tgtcttcagg ggcgtccgga
     2701 agtggatccc ctcctggagt gcgatgtcct ggatctcgcc ccacttggtg accactccga
     2761 ggttgccggc gtcgacctgg gtgcagcccg ataagccccc ggtgaaaaga gtcatcgcga
     2821 aaacaggtag ccatttgtct ttcaaattca tgtacataaa cccttctcta gagccacaag
     2881 cggaaatgtc cccccgagcc gctcaccaat ccagaatcgc caccatgaca ccggtcgcaa
     2941 cgatcagggc gcccgcggtg atgtccccat agcgctcgat ccacttgccc ttgaagagct
     3001 gtgcgcctga atgcgccaga ccgacgagaa ccaccatggc gcccactgtc gcgatctcgt
     3061 atgcgaccac gatggtaacg gtctccgccg tagtgagtgg ggccgctgca aacatgattg
     3121 ggatcacggc gacacagggg tctgcgcagt agatcaaaaa gagtgaccaa gtcgatgcgc
     3181 ccgaggcatc gtgtacgtga tcgtagtgat gatggtgatg accgtgaagt cgatgtgcaa
     3241 aggcgccgcg caatccccat atcgcatacg ccacaccgaa gccgatcaag agaagacccg
     3301 ccaccgagac catgcgctcg ccgagggact gaaacatctg cgcaccaaac atgagtgcga
     3361 gcagtccgag caacacggac acagttacgt gaccgaagcc acagatgaat gccacgcgca
     3421 tggctcgcgc cctcggccag ttgcgcgcgc gcgcgaccgc cgcgatcgga agccaatggt
     3481 ctggggccaa agagtgtaat gatcccaggc tgatagctcc gatcgcgagg gaagcgaaga
     3541 ggctagtggt gctcatggga atgctcgtgc ttttgcattg ttcggccctc gaccggcgac
     3601 ggccggtgct ccgccaacca ggtcaaccag gcgtcgatcc cttctcctgt ctttgcggaa
     3661 accttgagca tcgccgcgtt gggcatcacg cgcgcgagcg cgtcttcgac cgccgagacg
     3721 tcgaagtcga gatgcggaat caagtcgatc ttcgtgagca gaaccaagtc ggctcgttga
     3781 aacatcaccg ggtacttgag cggcttatct tcgccctcgg tcactgaaag tgccacgacg
     3841 ttcgcggctt gcccgagatc gtagatggcg ggacagacca ggttgccgac attctcgatg
     3901 aagaagatgt cgcgatcgct cgaggggaag tgatgcagcg cgtgatggac gagtcgcgca
     3961 tcgaggtgac aggcagaacc agtggtgatc gtggccgaca cgattcccga tgcattcaat
     4021 cgatcggcgt ctcgttcggt cgcgaggtct ccgctcaccg cgccgagttt caggtccttc
     4081 cccgcggcct tggccgtggc ctcgagcaag gctgtcttgc ctgctccggg cgagcccatg
     4141 agattgatcg ccaacaaccc taagccttcg aaatgacttc ggttatgggc agcctgggta
     4201 tcgttgtccg cgaggattcg ctcctgcacc tcgacgggga cgatttccgg atcaccacag
     4261 ccgcaggttt cacacatcag gggcctccat ttcgattcgt tccaggatga tctcgtcacc
     4321 ctgaaccatg cgggcggggc gcccgcaggt gtcacacctc aagatcgcgc cgcgctcgac
     4381 cgtgcgctta cagctcgggc aagcccaggt cgctgagaca gcatggatca aaagctcggc
     4441 gccgtcacag atcgttcgct cgcgaaacgt gtcgaacgcg gttttcaaca aatcgagctc
     4501 gactccggcg agctccccaa tcttgacgtg caagcggtgc acacgcgagc ttccacgagc
     4561 ttctgcttcc tgctgcacgc ggtcgatcaa agaggcgacg atgctgtact cgtgcacgcc
     4621 ctactcgctt tcgaattctg gtgcgcgttt ttcgaagaag gcagaaatgc cctcggcaaa
     4681 gttggcgcca tctgcgatgc gagccagctc ggtgagctcg gcgtccaagt gaacgtcgag
     4741 ccggtccatt ccagcggcct ggttgagaag attctttgcg acgcccaccg ccgcagtcgg
     4801 ggcgtcggct agctgggtcg cgattcgaag cgcctcggaa tccagtgact catggggaaa
     4861 aacgcggttg ataagccccc aatcgagcgc ctcaactgcg ctcaggcggg ggttcaggag
     4921 caccagctcg agcgcttttc gaagacccag aagccgtggc agaaagaagg tcgagctctc
     4981 cgccccggtg aggcccgttt tgaaataagc ccattcaaac caggcgcgat cggatgccaa
     5041 gacgagatcg cagctcatcg caatgccaaa gccgccagcc gccgcaggcc catcgacgac
     5101 tgcgatgaac ggtttcggtg cgcgccggat ctcggagatc gtgctgtgaa tgtattcgag
     5161 gatctgcttg aagatctccc cgtagccttc atttggttgc cgcgttcccg gctgcaggta
     5221 gccgaggtct gcgtcgtcgc cgccgtcgcg gatgtacttc aagtctgccc cgctgcagaa
     5281 gacgccgccc tccgctcgaa cgagtaccgc ccgcacgtcg cgatcacgcg cgagttgcag
     5341 acccgcgcga cggaagtcct gcgcggtctc tacgtcgagc gagttgagtc gctcgcggcg
     5401 gtcgatcgtg agaatgccca cggcgccgct tcgctcgact ctgatgtgct tcatcgctct
     5461 catcgctcag cagatcctcg gaaggagctc cccttcggcc tccgccagca atcggcttcc
     5521 aaagccggtg tcgaggatca cgaaaccggg cctttcggcg acgcattcac ctatgattgc
     5581 agcgtttcgt cccaaaggat gggaccgaat cgcgtcgaga accgcctccg cctgctcggg
     5641 ccggagccca aagacagctt ttccctcgtt ggccgacacc aatggatcga tgccgagcaa
     5701 ctcacccacg gagcgcacgt cgtcgcgaag cgggaccgaa gtctcgtcga tgatgattcc
     5761 gaccccgctc ttctcggcca tctcgtgaag tgcgctcgag aggccgccgc gcgttgggtc
     5821 cttgagcgcc acgacgccgt tgccgccgac gtcgagcgca tgccgcacca gcgtgttgac
     5881 gggcgcaacg tccgattcga gactcgtctc caaggcaagc tcgttgcgtg tcgccatgat
     5941 cgcgagccca tggtctccca tcgttcccgt cactagaatc ttgtccccag ggcgaaggcc
     6001 agcatccgaa accaatcgct tggtgagcgc gacgccggtc gtgttcagca ccagcccgtc
     6061 gagctcgcca tgacccatga ccttggtatc gccggttacg atcgtcgtcc cggcctcctc
     6121 gcaggcggat cgaatcgatt cccaaatccg tttgagcgta tcgcgcggga agccgtcctc
     6181 gatgatcgcc gcgcaggtca acgcaagcgg ctccgtagca cccatcatag cgagatcatt
     6241 gacggttccg caaactgcca ggcggccgat gtcaccgccg ggaaactcga tcggctggac
     6301 cacgtgcgag tccgtcgcga tgacgaggta ctggtcgccg atccggatcg cagcgccatc
     6361 atccatcgcg gcaaggccga tgccgtcggg ccccacgtca cccaaaccct ttgcgaagac
     6421 ctgctcgatc agtgcccgca tcgcgccgcc gccggttccg tgtttgagcg tgatccgctc
     6481 gtcgatgaac cgcgcgttgc gtgtcatgcc gcgcctcgct tgatcgaacg gagatcaggg
     6541 cgcccgccgt actgatgcca gatcttgcac gtgccttcgg cgctgaccat gcaagcgccc
     6601 actggcgtgt ccggcgtgca ctctttcccg aagagctggc agtccgacgg cgacgcgatg
     6661 ccggccatga tgtcgccaca ctggcactgc tgcacgagct ttggcggcgc gtagctccaa
     6721 agcgcagatg cgtcgatggt gaaccgcttc cgcgtatcga acttcgcgaa ctcgtcgcgc
     6781 agacggaggt tgccattggg cacgtgcgcg atccctcgcc agcgcccccc ggtgggccga
     6841 aaaacacgcc agagcatctt ctgcgcctga acgttccctt cccgcgttac acaacgggga
     6901 aacgcgtttt cgacgcgtgg ttcaccgcta ttgatcacct cgagcaactt cacgagaccc
     6961 gccaggacat cgagtggttc gaagcctgcg atgacgaccg gcaacccgtg acgctcgacg
     7021 aacttctcga acaccgcgga gcccgtgatg gtcgctgcgt gaccggctgc aaggaagcct
     7081 tcgatcttgg ctcccggcat ctctgccacg atctccatca ccggagggat gtacttgtgc
     7141 gccgagagca cggagaagtt atccggggga tcgtccagca agactgcggc tgtcgcgacc
     7201 gctgtcgttt cgaagcccga tgcgaagaag acgatgtcct cgtcgtgctc gcgcgcgaga
     7261 tccacagcct gggttacgct gtacaccaca tcgactttcg caccctccgc tttcgcgtcg
     7321 gcgagcgatt gtgaggtgcc cggtacgcgc agcatgtcgc cgtacgaagc gatccgaacg
     7381 ccctgctttg cgagtgccac ggcttcgtcg acttcgggaa catccgtcac gcagactgga
     7441 cagcccgggc ccatgatcac ctgcaattcc tgggggaaag ctgccctcaa gccaaacttc
     7501 gcgatcgcct gctcatggct gccgcagacg tgcatcaccg agaccggcgc gtgatccgct
     7561 ccttgtacga ggcgctcgag cgcatcccga agtccgcgcg ctcgcgcggg gtcccggaac
     7621 ttcagctgag cgaacgcctc acttggattc atcggtcgct ccgtcccgcg tcggtgtggc
     7681 atctggcgcg gcttcgatct cagcgcgaat gtcggccgtc atcagatcct gatcgtccgc
     7741 gagccggaga agctcgtcgt acagagcaag agtctcagcc gcctcttcgg caggaatgcg
     7801 gcgaatcgcg aatccgacat gattcaggat gtagtctcca ggagcgacag gcgcatcgca
     7861 gacctccaga cggacctgct tcttcacacc ccagaaatcc accgtcgcga tctgcatctc
     7921 gcggtcgact tcgatcacct tgcctggaac gcctaagcac atggaaaagc tcctctgagc
     7981 atcaaccttt gtctcccaga attgcgtccg cgatcaaggc ctgaccgagg gcgattcctc
     8041 cgtctcctgg tggaacctcg cgatgccgca acactctgtt tttatgagct aatttctcct
     8101 cgattccgcc agcaatccgt gcgttctgaa aaacaccacc cgtcagcgcc acaggatgcg
     8161 ctccgcactc tgccagaaga gcctccacga ccgctaccgt gaccgtcacg acggtattgt
     8221 ggaaacgggc cgacacgtct cccgccgcga ccccggagag cagatcagcc accgccgcgc
     8281 gcacagcggc tcgcaggtct acctcgattg gggttgcgct ccggtcgatg tcgaagggat
     8341 acgcatcgcg cacgttcgcg ttggcggcct gatcccaagc gatggctacc tgaccctcgt
     8401 atgcagactg aggacggcaa aggccaagcg ctccaaatcc gtcgaagtag cgcccgaggc
     8461 cgcgtgcttt cggtgaattc aaatcgcgtt cgagcatttg ggaaacgatt cgaagatgtt
     8521 ccggggcgat accttcgaag accgcgagcg cctcgatcgg aggcgcgccg tcgaaggcgt
     8581 cgtgcacgag cgcgtaagcg atgcgccata cttcccgcat cgccacgttg ccgcctgcca
     8641 agcgcagcgg ccggaagctc gccagtcgct cgtagcgtcc acgaaaaaca aggagaaact
     8701 caccgcccca ggatgttcca tcctcaccaa acccggtgcc gtcataggcc acgccaagca
     8761 cggggccttc gattccggac tccgcaatga gacttgctac gtgtgcgtga tggtgctgaa
     8821 caccgactcg cgtcgctcca gttctcgttc ctgcgtatcg agtcgagatg tagccgggat
     8881 gaagatcatg ggcgacgacc tctggacgca ccgagagaat cgtttccatt ctgtctatgg
     8941 actcctcgaa gaactcgagg gcggcgagcg attcgagatc tccgatatgc tgtccaaagt
     9001 acgcgctgtc tccagtcgcg atgcagaagg tgttcttgag gtgccctccg caagcgagaa
     9061 caggacgcga aaccggcttg gccaaacgga agggtcgcgg gacgaaaccc cgtgaacggc
     9121 gcaggatcgt cggcacgccg gcgacgactc tcacgaccga gtcgtcgcaa cgcatcgcga
     9181 tatcgcgatc gtgcaggaga accgcgtcgg cgattccctc cagtcggtcc tgggcttcgt
     9241 cgttgtcgcg gcagatcggc tcctccgcta cgttcgccga agtcatcacc agggggcggc
     9301 ccgcggccgc gaggaggagg tgatgcagtg gcgtgtaggg caaaaacagg cccaaaagag
     9361 gtgtgtccgg gttcacagca gcagccaagc tcgaatcggc gcgcgctcga acgagaacga
     9421 tcggcctctc ggctgagctg agtagggctt cttcctcggg atcgatttcc gcaatcgcgt
     9481 gggccgcatc gagatccgcg accatgacgg caaacggttt ctcgtctcga tgcttgcgct
     9541 gacgcaagcg aaggacggcc gcttcgttcc aggcatcgca ggaaaggtga aagccgccta
     9601 ggcccttaac cgccacgatc gagcccgaca tcagaagctc agctgcctcg gccaagggat
     9661 tgtcggtgga gcacggcgtc ccgtccgagc gtaccaagtc gagagagggg ccgcactgag
     9721 ggcatgcgtt gggttgcgcg tggaaacgcc ggtcttcgat gtcgtcgtac tcgcgtcgac
     9781 atgcgtcgca catcgggaaa gttctcatcg tcgtcgcgga gcggtcgtag ggaatgccag
     9841 tcgcgatcgt gaagcgcgga ccgcagaccg tgcagttggt gaacgcgtag ccgaatcgcc
     9901 ggtcctccgg gtccatcacc tcggcgcggc aatcgacgca ggtcgtgagg tcgggcggga
     9961 tcgacagcgc cttgtctccc gaggctccgc tcgattcgat cgcgaaaccg tcgagatcct
    10021 ggaccgggat cggtgccgcg cgcagctcga cgatgcgcgc cgaaggcggc gggttcgact
    10081 tcaactcctg cacgaagcgc tcgagaattt cctccggtgc gaacgcttca atcgtcactc
    10141 cacgtgcatc attacgcacg cgtcctacga cgccggacct ccgtgccatg cgatacaccc
    10201 agggccgaaa ccccacgcct tgcaccgttc cgcgaatctc gatgcggcgc ccggtgttca
    10261 cggcccgtcc tcagccacgc tgagaacgta cgcgatgagg tcccacttct cctcgtcgcc
    10321 gagcgtcggc caggcgggca tcgaggttcc ccgcttgccc tcgctcagga cctcgaagag
    10381 cgcaagcggc gaggtgctcg caagccattc cttgttctgg aagttgatcg gcttgcccga
    10441 gagccccttg cttcggacgc cgttcccatc cgctcgctcc ccgtggcaaa gcgcacactt
    10501 ctttcgatag agcgccctcc cgcgtgcccg ggcatcgtcg gaggcgaggc gttcaatcgg
    10561 aacttggact tcgctgtagt cgctgggcga cgaatcatcc ctggcgctac acgccaagag
    10621 tgcgagcgac gtgagcagcg ctcccataag cttcatcgcg attctccgat cggaccaccg
    10681 gtgggtatcc catcgcggtc agctcttcga cgacgcgctg gaccagctcg ggaatgcttg
    10741 cctgcaccgc cggcgtccgt tcgagcccca gctcgatcga ctccggaacg acacccagca
    10801 ccaaaacgcg gtccgggtac cggtcgagca ggctcgcgcc cgcgagcagg tcggcgacac
    10861 caatctgatg aggcgaaaga cgctcgtaca cggcgggagc gacctcgtcc ccttcgattc
    10921 gaacctgggt gcccggcggc ccgtccgccc tgatcgcgtc caccagaatc acctgatcgg
    10981 cgctgtcgac cagaggcaga agcgagagcc cgagggttcc
//
LOCUS       DASRZF010000023         6337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4488428, whole genome shotgun sequence.
ACCESSION   DASRZF010000023 DASRZF010000000
VERSION     DASRZF010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 6337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6337
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4488428"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VFG22_01150"
     CDS             complement(<1..501)
                     /locus_tag="VFG22_01150"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET6414871.1"
                     /translation="MSSDSMILTVANRLSRHPPFDRLDSELLDRLARGIRIRYLEADE
                     VVFEEGGQAKPEFYLVVKGEVMISQQVEGQTVLIDVCDDGDIFGVRALLADRPYGGRA
                     QAKDDTLLYVLSKAQLDELIRDAPRVALFFAADFAADVPQRLETNRLLAVRAARRHEK
                     VRDAADD"
     gene            complement(507..3122)
                     /gene="pepN"
                     /locus_tag="VFG22_01155"
     CDS             complement(507..3122)
                     /gene="pepN"
                     /locus_tag="VFG22_01155"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HET6414872.1"
                     /translation="MTGRNHDRIYRRDYQPSDFVIDTVDLSFELDPKDTVVRAKMTLR
                     RNPDSSASAGPLVLHGDELELLHIRIDGVALDSQQFEADSQSLRIHEPPAQFLLETEV
                     RIRPADNTKLMGLYTSSGAFCTQCEAEGFRRITYYLDRPDVMARFTTRIEADRAKYPV
                     LLSNGNRVGQGELPNGRHWVRWEDPFRKPAYLFALVAGDLVCHRGEFTTASGRKVVLE
                     IWVEPENADKCEHALASLRAAMKWDEDTYGLEYDLDIYMIVAVNDFNMGAMENKGLNI
                     FNSKFVLAKPSTATDDDYEGIESVVAHEYFHNWTGNRVTCRDWFQLTLKEGLTVFRDQ
                     EFSADMTSRAVQRIKDVTRLRTAQFAEDAGPLAHPIRPESYIEMSNFYTATVYEKGAE
                     VVRLYQTLFGKEGFRKGLRLYLETHDGRAVTCDDFRQAMADANQADLSQLERWYSQAG
                     TPEVHARGAWDESAQTFALTLTQTQRRLQGQSDPLPLPIPLRVGLLGSDGRDLPGAAR
                     TLQLTERAQTFTFEQVTERPAASIARGFSAPIKLEVERNAEELAFLMAYDSDPFNRWE
                     AGQDLAKHVLLGLVARVAEGSPLELDAGLAHAFQNVLSDPRLDGSIKALTLTLPSEEI
                     LAQALEVVDPEAVHVARRFLIRELARSLRHELTEIYEENQSGTDAFSKTEVDRRRIKN
                     RALRYLVALEEPETIAMAVAQFDEATGMTDYEAAFASLVDIVSDETEDAIEAFHERWQ
                     SEPLVLDKWFRMQAMASTASAFDRVVALFRHPDFTLNNPNRARSLLYAFAAGNPIGFH
                     RADGAAYRFIADRVLELDAINPQVAARIVGSFNQWKRYEPGRSILMKAELERIGSHSK
                     VSKDVAEIVERALEN"
     gene            complement(3167..3580)
                     /locus_tag="VFG22_01160"
     CDS             complement(3167..3580)
                     /locus_tag="VFG22_01160"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HET6414873.1"
                     /translation="MNPNLILWPVVVQIALTAYVYIRLKNVKVQAVKTEGVDRQKRAL
                     HPDAWPDSVIQVNNNIRNQFELPVLFYVVTLIIWELGAVNPVTLGLAWLFALSRIVHA
                     YIHTGPNIVKYRLRAFTVGFLALLGMLGAIIHALV"
     gene            3621..4472
                     /locus_tag="VFG22_01165"
     CDS             3621..4472
                     /locus_tag="VFG22_01165"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6414874.1"
                     /translation="MENDVEMKNFQDKVAAITGAGSGIGRALALDLAGRGCHLALSDI
                     NEVGLAETVRQIGTPGVRVTAQVVDVADRRAVYDWADQVVAEHGKVNLIFNNAGVALG
                     STIEGMSYDDIEWLMNINFWGVVYGTKAFLPHLKASGEGHIANVSSVFGLAGIPTQGA
                     YNAAKFAVRGFTECLRQELDMTDSGVSATSIHPGGIKTAIARSARMNSNIADLGLDVD
                     GSTAKLERGFITSPEKAARIILRAVERNQRRVLVGPDAYVFDWMVRLLPSRYQDIATR
                     LSKLTMR"
     gene            complement(4486..5436)
                     /locus_tag="VFG22_01170"
     CDS             complement(4486..5436)
                     /locus_tag="VFG22_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6414875.1"
                     /translation="MQTLELLDGTKMPALGLGTWKSEPEAVYGAVRSALEVGYRHIDC
                     AAIYQNEEEVGRALGDAFQAGDAKREEVWITSKLWNDSHAPEQVRPALETSLRKLQLD
                     YLDLYLIHWPVALKPGTMLPRGPQDFVPLSEIPLEATWGAMSKVSEQGLARQVGVSNF
                     SQRMIARVSDATGVTPAVNQIELHPYLQQNARVAVCKERGIGVTAYSPLGSPDSAAMM
                     GRKDEVLLTRHPTIVEIAESHGATPGQVLIAWALVRGTSVIPKSVNPGRIAENWASQR
                     LALTPEDMRAIAALDRHERMVDGRFWFIGETYSAETLWDE"
     gene            complement(5450..6238)
                     /locus_tag="VFG22_01175"
     CDS             complement(5450..6238)
                     /locus_tag="VFG22_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414876.1"
                     /translation="MTLPLLVAIAGFFAVLGTSVFVAWLAFSAGRWLGSREALRDDRE
                     HEGPVGSVVGATLGLLAFTLAITFNMAHTKYNDRKQLVREDVRAIETAYDRALLLPED
                     ASHAAHEKLVEYARIRAFDTFANPEVPIAAIIARSEKIQQELWMQALDHRDHPQIRFY
                     MDALSVMRNIHAERVTVGIHDHVPIPVWIALIFVTALGMVTMGYQASLVGSKRARAMI
                     PLIVAFCVILTMALDLDRPQQGILRVDQGPMIRLDASLEHRGDR"
BASE COUNT         1235 a         1908 c         2002 g         1192 t
ORIGIN      
        1 atcatcggcg gcgtctcgca ccttttcgtg acgccgcgcg gcccgcaccg ccaggagtcg
       61 attggtttcg agacgctgtg ggacgtcggc agcgaagtcg gccgcaaaga acagcgcgac
      121 cctcggcgcg tctcgtatca gctcgtcgag ctgggctttc gaaagcacgt agaggagagt
      181 gtcgtccttg gcctgcgcac gcccgccata cggacgatcg gcgagaagcg cgcggactcc
      241 gaagatgtct ccatcgtcgc aaacgtcgat gagcacagtc tgcccctcga cttgctgact
      301 gatcatgacc tcgcccttga ccaccaagta gaactccggc ttggcttgtc cgccttcctc
      361 gaagacgacc tcatcggctt cgaggtagcg aatccgaatc ccgcgagcga gtcggtcgag
      421 cagctccgag tcgagccggt cgaagggcgg atgcctcgac aacctgttcg cgacggtcaa
      481 gatcatcgaa tcggacgaca tgttcgctag ttctccaggg cgcgctcgac gatttcggcc
      541 acgtctttcg agactttcga atgggagccg atacgctcga gctcggcttt catcaagatg
      601 ctccttccgg gttcgtagcg tttccactgg ttgaagcttc cgacgatgcg agcagcaacc
      661 tgcgggttga tcgcgtcgag ctcgagaacc cgatctgcga tgaatcggta agcggcaccg
      721 tctgcccggt gaaagcctat cggattgccg gccgcgaacg cgtagagaag cgagcgagcg
      781 cgattcggat tgtttaaggt gaagtcggga tgccggaaca gcgccaccac ccggtcgaat
      841 gctgacgcag tgctcgccat cgcttgcatg cgaaaccatt tgtccaacac cagcggctcg
      901 ctctgccacc gctcgtgaaa tgcctcgatt gcgtcctcgg tttcatccga cacgatgtcg
      961 accagagatg cgaacgcggc ttcgtaatcc gtcattcccg tagcttcatc gaactgggcc
     1021 acggccatgg cgatcgtctc gggctcctcc aaagcgacca ggtagcgaag cgcacggttc
     1081 ttgattcggc gacggtcgac ctcggttttc gagaacgcgt cggtgccact ttggttctct
     1141 tcgtagatct cggtgagctc gtggcgcaac gatctcgcaa gctcgcggat caaaaaacgc
     1201 cgcgcgacgt gaacggcttc cgggtcgacc acctccaggg cttgagccag aatttcctca
     1261 gagggcagcg taagggtcag cgccttgatc gaaccatcga ggcgcgggtc ggagaggacg
     1321 ttctgaaacg cgtgggcgag gcctgcgtcg agctcgagcg gactgccttc ggcgacgcgt
     1381 gcgactagac ccagcaagac gtgtttcgcc aagtcctgac cggcctccca gcgattgaag
     1441 ggatcggaat cgtaggccat caagaaggcc agttcttccg cgttgcgttc gacttcgagc
     1501 ttgatcggcg cagagaagcc tcgcgcgatg gaggcggcag ggcgctcggt cacttgctcg
     1561 aatgtgaagg tctgcgcgcg ctccgtgagc tggagcgtac gggctgcgcc gggaagatct
     1621 cgaccatcgg aaccgagcaa gccgacccgg aggggaatgg gtaagggcaa gggatctgac
     1681 tgcccttgga ggcgtcgctg cgtttgcgtc aacgtgagcg caaaggtttg ggccgactcg
     1741 tcccaagctc ctcgagcgtg aacttcgggt gtgccggcct gcgaatacca gcgctcgagc
     1801 tggctcagat cggcttggtt ggcgtctgcc atcgcttgcc gaaagtcgtc gcaggtgacc
     1861 gcccggccgt catgcgtctc gagatagagc cggagaccct ttcggaatcc ttccttgccg
     1921 aagagcgtct ggtagaggcg cacgacctct gcgcccttct cgtacacggt cgccgtgtag
     1981 aagttactca tctcgatgta cgactccggc cgaattggat gcgcgagcgg ccccgcatcc
     2041 tcggcgaact gagcggtccg aagccgagtg acgtccttga tgcgctgcac ggcgcgggac
     2101 gtcatgtcgg ccgagaactc ctggtcccga aacacggtca gaccttcttt gagggtgagt
     2161 tggaaccagt cgcggcaggt cacgcgattc ccagtccagt tgtgaaagta ctcgtgtgcg
     2221 accacgcttt cgatgccctc gtagtcgtcg tcggttgcgg tcgaaggttt ggccagtacg
     2281 aacttcgagt tgaagatgtt gaggcccttg ttctccatcg cgcccatgtt gaaatcgttg
     2341 acggccacaa tcatgtagat atcgagatcg tactcgagcc cgtaagtatc ctcgtcccac
     2401 ttcatggccg ctcgaaggga cgcaagcgcg tgctcgcact tgtccgcgtt ctctggctcg
     2461 acccaaatct cgagcacaac cttgcggcct gatgcggtcg tgaactcgcc ccggtggcaa
     2521 accaaatccc ctgccaccaa ggcgaacaga taggcgggtt tgcgaaaggg atcttcccat
     2581 cgaacccagt ggcgtccgtt cggtagctcc ccctgcccga cccgattgcc attggagagc
     2641 aggacgggat actttgctcg atcggcctcg atgcgcgtcg tgaaccgggc catcacatcc
     2701 ggcctatcca gatagtacgt gatccgccga aagccctcgg cttcgcattg ggtacagaag
     2761 gcgccactcg aagtgtagag gcccatcagc ttcgtattgt ccgccggacg aatccgcact
     2821 tcggtctcga gcaggaactg cgcgggcggc tcgtgtatcc gcaagctctg cgagtcggcc
     2881 tcgaactgct gcgaatccaa cgcgacaccg tcgatccgaa tgtgaagaag ctcgagctcg
     2941 tctccgtgca agaccagcgg cccggcgctc gcggacgaat cgggattccg ccggagggtc
     3001 atcttggcac ggacgacggt gtcctttgga tcgagctcga acgagaggtc gacagtgtcg
     3061 attacgaagt cggaaggttg gtagtcccta cgataaattc ggtcgtggtt gcggccggtc
     3121 atggtcggag cttagtctag accgagcccc ggcgaaccgc attctctcag acgagcgcgt
     3181 gaatgatggc gccaagcatg ccgagcaggg cgaggaatcc gacggtgaac gcccgaagac
     3241 ggtatttgac gatgttgggg cccgtgtgga tgtacgcgtg tacgatacgg ctgagggcaa
     3301 acagccaggc caggccgagg gttaccggat ttacggcccc gagctcccaa atgatcaggg
     3361 tcaccacgta gaacaacacc ggcaactcga actggttgcg tatgttgttg ttcacctgga
     3421 tcaccgagtc gggccatgcg tccggatgaa gcgccctctt ttgtcgatcg acgccttctg
     3481 tcttgaccgc ttggaccttc acgttcttga ggcggatata gacgtacgcg gtcagagcga
     3541 tttgaaccac gacgggccag aggatgagat tgggattcat ggctggcgaa gaataccggg
     3601 ttgacccgag ggtccgtcca atggaaaacg acgtcgagat gaaaaacttc caagacaaag
     3661 tggccgccat cacgggtgct ggctctggga tcggtcgtgc cctagccctc gatctggcgg
     3721 gacgcggctg ccacctggcg ctctcggaca tcaatgaagt cggcttggca gagaccgtgc
     3781 ggcaaatcgg aacgcccggc gtacgggtga ccgctcaggt ggtcgacgtg gccgatcgtc
     3841 gggcagtgta cgactgggcc gaccaggtcg tggccgagca cggaaaggtc aacctgatct
     3901 tcaacaacgc gggtgtggcg ctcggcagca cgatcgaggg gatgagctac gacgacatcg
     3961 aatggttgat gaacatcaac ttctggggag tcgtctacgg caccaaggca ttcttgccgc
     4021 acctgaaggc gagcggcgag gggcacatcg ccaacgtttc gagcgtgttc ggattggcgg
     4081 ggatcccgac ccagggcgcg tacaacgccg cgaagtttgc agtccgaggg ttcacagagt
     4141 gcctgcgtca ggagctcgac atgacggact ctggagtcag cgccaccagc atccatccag
     4201 gcggaatcaa gacagccatt gctcgctctg ctcgaatgaa cagcaacatc gcggatctgg
     4261 gattggatgt cgacgggagc accgcaaagc tcgagcgagg attcataacg agccccgaga
     4321 aggccgctcg aatcatcctc cgcgcagtcg aaaggaacca gcgccgggtg ctggtcgggc
     4381 cggacgccta cgtcttcgac tggatggtcc gacttctgcc gtcgcgctac caggacatcg
     4441 cgacacgtct gtcgaagctc accatgcgct gaggctcggc cggcctcact cgtcccagag
     4501 ggtctcggct gaataggtct ccccgatgaa ccagaagcgt ccgtccacca tgcgctcatg
     4561 cctatcgagg gcggcaatgg cgcgcatgtc ctcgggagtc agagccagcc tctgtgacgc
     4621 ccagttctct gcgatccgcc ccgggtttac ggatttcggg atgacgctcg tgccacggac
     4681 cagagcccag gcgatcagaa cctgccccgg tgtcgctccg tgcgactcgg ctatctcgac
     4741 gatggtcggg tgccgggtga gcaagacctc gtccttgcgc cccatcatcg ccgcggagtc
     4801 gggagacccg agcggagagt aggcggtgac cccaatcccg cgctccttgc agacagccac
     4861 acgagcgttc tgctgaagat agggatgaag ctcgatctgg ttgaccgccg gggtgacgcc
     4921 ggttgcatca ctgacgcgag caatcatgcg ctggctgaaa ttggaaaccc caacctgccg
     4981 cgcgagccct tgctcggaga ccttcgacat cgctccccac gtggcctcga gcggaatctc
     5041 cgagagcggg acgaagtctt gcgggccgcg tggaagcatc gtgccgggct tgagggctac
     5101 cggccaatga atgagataga gatccagata gtcgagctga agctttcgga ggctcgtctc
     5161 gagcgcgggt cgcacctgct cgggcgcatg cgagtcgttc cagagcttgc tggtgatcca
     5221 aacctcttcg cgcttcgcgt cgccggcctg aaaggcatcg cccagggcac gcccgacctc
     5281 ttcctcgttt tggtaaatgg cggcgcagtc gatgtgtcga tacccgacct cgagggcgga
     5341 gcgaaccgcg ccgtagacgg cctccggttc ggatttccag gtgccgaggc ccagcgcggg
     5401 catcttcgtt ccgtcgagta gctccaaggt ctgcatggtt ccctccgaat cagcgatcgc
     5461 ctcgatgctc gagcgaggcg tcgagccgaa tcatgggacc ctgatccaca cgcaggatgc
     5521 cctgctgggg gcggtccaag tcgagagcca tggtgaggat gacgcaaaac gcgacgatca
     5581 acgggatcat cgcgcgcgct ctcttcgaac ccaccagcga ggcctgatag cccatggtga
     5641 ccatcccgag ggcggtcacg aagatgagcg cgatccagac cggaatcgga acgtggtcgt
     5701 gaattccgac cgtgacacgc tcggcgtgga tgttcctcat gacgctcagc gcgtccatgt
     5761 agaaccggat ctgagggtga tcacggtgat cgagcgcctg catccacagc tcctgctgga
     5821 tcttctcgga ccgggcgatg atcgccgcaa tcggcacctc ggggttggcg aacgtgtcga
     5881 aggcccgaat ccgagcgtac tcgaccagtt tctcgtgcgc cgcgtgggag gcgtcttccg
     5941 gcagaagcaa ggcgcggtcg taggcggtct cgatcgccct cacgtcctcg cgaaccaact
     6001 gcttgcggtc gttgtacttg gtgtgagcca tgttgaaggt gatcgccaag gtgaatgcga
     6061 ggagcccgag ggtggcgcca acgaccgagc caacggggcc ctcgtgctcg cgatcgtctc
     6121 gcagcgcctc gcgcgaaccc agccatcgtc cggcggagaa cgccagccag gccacgaaaa
     6181 ccgaggtacc tagaaccgcg aagaacccgg ctatcgcgac tagcagcgga agggtcatga
     6241 caaacgatcc atgcttggac tcaggcgtat atcgcagtcg ggaggcgcgc tagcaacttg
     6301 cctggaagga ggctgggaat tcagccgagc atcgcat
//
LOCUS       DASRZF010000024         4913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4488635, whole genome shotgun sequence.
ACCESSION   DASRZF010000024 DASRZF010000000
VERSION     DASRZF010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 4913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4913
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4488635"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="VFG22_01180"
     CDS             <1..695
                     /locus_tag="VFG22_01180"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF003916.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HET6414877.1"
                     /translation="LRKDLLEANGAIFTGQGKALSDVASRDVKVLVVGNPANTNSYIA
                     MKNAPDLDPSCFTAMVRLDQNRAMSQVASRTGRAVTDVKRVTIWGNHSATQYPDLFNA
                     QMDGKSVAALINDQAWIENDFIPTVQERGKAIIEARGLSSAASAANAAIDHIRDWHLG
                     TADGDWASMAVPSDGSYGIPEGLMFGFPCTCSGGNWSIVQGLEINEFSRAKLDATAAE
                     LQEEAAAVAHLV"
     gene            728..1627
                     /locus_tag="VFG22_01185"
     CDS             728..1627
                     /locus_tag="VFG22_01185"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6414878.1"
                     /translation="MQKPSLVEEHEAAGKRIVVDGHHIFVREEGEGEPVLLLHGVPSS
                     SFLFRKMLPELAGHGLRAVSFDFPGVGLSDKPKGIDYDWHALAKWVGRVVDALELQPV
                     HLVLHDIAGPIGSEWAIQNPVKVRSITVTNTLMDVAHFAPPFPMWTFRVPVMRHLAFS
                     TMNTSVMLKLFRHSGVKNPEAVDESVIDSYIYLLKNNGGHHSFLEIMQGFDLSEKHRD
                     YLRDGLLAIDKPMQLVWGEQEIAIPKAQLHYIKQVFPLRAEHMVDARHFLQEEQPAVI
                     AAHIAAFAADIAQPSKRATNAPA"
     gene            1687..2106
                     /gene="ndk"
                     /locus_tag="VFG22_01190"
     CDS             1687..2106
                     /gene="ndk"
                     /locus_tag="VFG22_01190"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HET6414879.1"
                     /translation="MAVERTLSIIKPDAVEQNNIGNILAMIQGAGLEILGLRMLHLSR
                     AQAEGFYEVHTERPFFGELVEFMTRGPVVVTALQADNAVARYRKLMGSTNPAEADEGT
                     IRKAYGTDIGENACHGSDSVENGRIEVSYFFPEYLLK"
     gene            2110..2943
                     /locus_tag="VFG22_01195"
     CDS             2110..2943
                     /locus_tag="VFG22_01195"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HET6414880.1"
                     /translation="MSESHESELRALVEQAFGDRTKLEGARYRSAVEETIELLDKGKL
                     RVASRGADGEWTVHAWTKQAILLYFGIRGMERVQVGAYEYHDKIPLKKNLDQQGIRVV
                     PPGTIRYGAHMEPGAILMPGYVNIGAYVGAGSMVDTWATVGSCAQVGKGVHLSGGVGL
                     GGVLEPPGAKPVIIEDGAFLGSRSIVVEGSRIGEEAVLGANVVITSSTKIIDVTGSEP
                     VELQGYIPPRSVVIPGTRNKKFPAGEYQVPVALIIGKRKTSTDKKVTLNEALRDFGVS
                     A"
     gene            3068..4060
                     /gene="dctP"
                     /locus_tag="VFG22_01200"
     CDS             3068..4060
                     /gene="dctP"
                     /locus_tag="VFG22_01200"
                     /inference="COORDINATES: protein motif:HMM:NF037995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HET6414881.1"
                     /translation="MRNILLLLVLGLLISGDSFTTQARAEASHQLRIATLAPRDSPLG
                     DVYQQLKTGMKERTGGQVNMKMYYGGVAGDEITVVRKMRVGQLDGALITSTGLSALVR
                     QVLVMEAPGLIRSYPELDAVREDLAPQLEALFDQAGYKLIAWGDAGKTRIFSTHKIYG
                     PSDLQNVRPWVWRDSATMRAFIKAAGANGVLLNLPEVYSGLQTGIIDTVIASSVGVLA
                     FQWHTKLKTMAKQAGGIVTGAFVIKKDHLDKLPKEARDYLDEVSVKTEEIFRVEGRKI
                     DDEAAITLSKKLKVINLFRAQRQWEEVQFTARESLVGRMYSRALLNRVQEILHK"
     gene            complement(4064..4486)
                     /locus_tag="VFG22_01205"
     CDS             complement(4064..4486)
                     /locus_tag="VFG22_01205"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HET6414882.1"
                     /translation="MRTIASIAIILALGSCSRTTPSAKPILDAASNTAEQNKEIARYL
                     IRNEYSQWDDARQFQCLDKLWQAESRWNHRARNKRTGACGIPQSYPCNKMSESGKTYG
                     VDYRKNPWPQIAWGLRYIEGRYGTPCAAWKRFQRGGGY"
     gene            complement(4501..4734)
                     /locus_tag="VFG22_01210"
     CDS             complement(4501..4734)
                     /locus_tag="VFG22_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414883.1"
                     /translation="MIRALFVSAGMAALVLACHASMPPEGAIRLQAVPTVCGELELAS
                     AGQGVDSRATLWTADNVSEMQASTRSFRAMSSS"
BASE COUNT         1010 a         1525 c         1484 g          894 t
ORIGIN      
        1 tgctccgcaa agaccttctg gaggcgaatg gcgccatctt cacgggccag gggaaggcct
       61 tgagtgacgt cgcaagtcga gacgtcaaag tgctggtcgt cggtaacccg gcgaacacca
      121 acagctacat cgccatgaag aacgcgccgg acctggaccc gtcgtgcttc accgcaatgg
      181 tccggctcga tcagaaccgc gcgatgtcac aggtggcgtc gaggaccggg cgagccgtga
      241 ctgacgtcaa gagagtcact atctggggca accattcagc gacgcagtac ccggacctct
      301 tcaacgctca gatggacgga aagagcgtcg cagccctcat caacgaccag gcctggatcg
      361 aaaacgactt catccccacc gtgcaggaac gcggaaaggc catcatcgag gctcgggggc
      421 tgtcgagcgc tgcttctgcc gccaatgccg caatcgatca cattcgcgat tggcatctcg
      481 gtaccgccga tggcgattgg gcctcgatgg cagttccctc cgacggaagc tacggcatcc
      541 ccgaagggct catgttcggt ttcccctgca cctgcagtgg cgggaactgg tcgatcgtcc
      601 aggggctcga aatcaacgag ttcagccgtg cgaagttgga cgccacggcc gcagagctcc
      661 aagaagaggc tgctgccgtc gcgcatctgg tttaggattc gaccgagcac gttccggggg
      721 tccccgcatg cagaagccca gcctggtcga agagcacgaa gctgcaggaa aacggatcgt
      781 cgtggacggc catcacatct tcgtacgcga agaaggtgag ggcgaaccgg tgctcttgct
      841 gcacggcgtt ccttcgagct cctttctttt tagaaagatg cttcccgagc tcgccgggca
      901 tggacttcga gcagtcagct tcgactttcc aggtgtgggc ctgagcgaca agcccaaggg
      961 catcgactac gactggcatg ccctcgcgaa atgggtggga cgggtcgtcg acgcgctgga
     1021 gctccagccc gttcatctgg tccttcatga catcgcaggg ccgataggct cggagtgggc
     1081 cattcagaac ccggtgaagg ttcggtcgat caccgtaacg aacacgctca tggatgtggc
     1141 gcatttcgcg ccgccgttcc cgatgtggac gtttcgagtc cctgtgatgc ggcatctggc
     1201 cttctcgacc atgaacacgt ctgtgatgct gaagctcttc cgtcatagcg gcgtgaagaa
     1261 tcccgaggcg gtcgacgaga gcgtgatcga ttcctacatc tacttgctca agaacaacgg
     1321 cggacaccac agcttcctcg agatcatgca gggcttcgac ctcagcgaga agcaccgcga
     1381 ctacctgcgt gacggccttc tcgcgatcga caaacccatg cagctcgtct ggggagagca
     1441 ggagatcgcg attccaaaag ctcagctcca ctacatcaag caggtgtttc cactgcgggc
     1501 cgagcacatg gtcgacgcac ggcacttctt gcaggaagag caaccggcag tcatcgccgc
     1561 gcatattgcc gctttcgccg cggacattgc ccagccatca aaacgcgcta caaacgcgcc
     1621 cgcgtagagt gccattggca ccgcggggct tcgccccact gaactgcacc caaaagagga
     1681 gcccacatgg ccgtcgaacg gacactcagc atcatcaagc ccgacgctgt cgagcagaac
     1741 aacatcggga acattctcgc catgattcaa ggcgcgggac tcgagattct cggtctgcgc
     1801 atgctgcatc tcagccgagc tcaggccgag ggattctacg aagtgcacac agaacgaccg
     1861 ttctttggcg agctggtcga gttcatgacg cgcgggccgg tcgtcgtgac ggcgctccag
     1921 gccgacaacg ccgtggcacg ctaccgaaag ctcatgggct ccaccaatcc ggccgaagcg
     1981 gacgaaggca ccattcgaaa ggcctacgga accgacatcg gcgagaacgc ttgccacggc
     2041 tccgacagcg tcgagaatgg ccgcatcgaa gtgtcttact tctttcccga gtacctgctc
     2101 aagtagccga tgagcgaatc acacgagtcc gagttgcgcg cactcgtcga gcaggcgttc
     2161 ggtgatcgaa cgaagctcga gggcgcccgg taccgcagcg ccgtcgagga gacgatcgag
     2221 ctcctcgaca agggcaagct ccgagtggcc agccgtggcg ccgatggcga gtggaccgtt
     2281 cacgcctgga ccaagcaggc gatcttgctc tactttggaa tccgaggaat ggagcgcgtg
     2341 caggtcggcg cgtacgagta tcacgacaag atccctctca agaagaacct agaccagcaa
     2401 ggcatccggg tcgtgccgcc cggcaccatt cgatacggtg cacacatgga gcccggtgcc
     2461 atcttgatgc ctggctatgt gaacatcggc gcctacgtcg gcgcgggatc gatggtcgat
     2521 acctgggcca cggtcggctc ctgcgcgcag gtgggcaagg gcgttcacct ctctggtggg
     2581 gtcggcctcg gcggggttct cgagccgcct ggcgccaagc cggtcatcat cgaggacggc
     2641 gcattcctcg gttcccgctc gatcgtggtc gaaggctccc gaatcggcga ggaagcggtt
     2701 ctcggcgcga acgtggtgat cacatcgagc acgaagatca tcgatgtcac cggttccgag
     2761 ccggtcgaac tgcagggcta cattccgcct cgctcggtcg tcatccccgg cacgcgcaac
     2821 aagaagttcc ctgctgggga gtatcaggtt ccagtcgcgc tgatcatcgg caagcgcaag
     2881 acgagtaccg acaagaaggt gacgctcaac gaagccctga gagacttcgg cgtctcggcg
     2941 tagcccgagg tcaacggccg tatcggtgac gccgcgtcac ggtacgactc catggcgaat
     3001 gacctgtttc ctcaaacgag ggcattctgg cgttgaagtt ggcggtgcat tcatgtttag
     3061 atagcggatg cgcaacattc tgctcttgct cgttctcggt ctgctgatca gcggcgattc
     3121 cttcacgacg caagcccgcg ccgaggcatc gcaccagttg cgaatcgcga cgcttgcacc
     3181 gcgtgattcg ccgctaggtg acgtctacca gcagctcaag accggaatga aggagcgcac
     3241 cggcggccaa gtgaacatga agatgtacta tggcggagtc gcaggggatg agatcacggt
     3301 cgtccgcaag atgcgcgtcg gtcagctcga cggcgcgctg atcacctcga ccggcctctc
     3361 ggcgctcgtt cgtcaggtgc tcgtgatgga ggcgcccggt ctcatccgga gctatcccga
     3421 gctcgacgcg gtccgtgaag atctcgctcc gcaactcgag gctctgttcg accaggccgg
     3481 gtacaagctc atcgcctggg gcgatgctgg caagacacgc atcttctcga cacacaaaat
     3541 ctacggccct tccgatttgc aaaacgtccg cccgtgggtc tggcgtgact cggcgacgat
     3601 gcgggcgttc atcaaggcag ccggcgcgaa cggcgttctg ctcaaccttc ccgaggtgta
     3661 ctccggactg cagaccggca tcatcgacac cgtcattgcg tcctcggtcg gcgtgctggc
     3721 cttccagtgg cacaccaaac tcaagaccat ggccaagcag gcaggcggca tcgtcaccgg
     3781 cgccttcgtc atcaagaaag accacttgga taagcttccg aaggaggcgc gcgactatct
     3841 cgatgaggtt tccgtcaaaa ccgaagagat cttccgagtc gaaggtcgca agatcgacga
     3901 cgaggccgcg atcacgctct ccaagaagct caaggtcatc aatctattcc gggcgcaacg
     3961 tcagtgggaa gaggttcagt tcaccgcgcg cgagtcgctc gtcggccgca tgtattcccg
     4021 tgccctcctg aaccgcgttc aggaaatcct tcacaaatag cggctagtag ccaccgccgc
     4081 gttggaagcg tttccatgcg gcgcatggcg tcccatatcg cccctcgatg tatcgcaagc
     4141 cccaggcgat ttgcggccag gggttctttc ggtagtcgac gccgtaggtc tttcccgatt
     4201 ccgacatctt gttgcaggga taggactgcg ggatgccaca tgcgccggtt ctcttgtttc
     4261 gcgcacgatg gttccaacgg ctctccgcct gccacaattt atcgaggcac tgaaactgcc
     4321 gcgcatcatc ccattgggaa tactcgtttc gaatgagata gcgggcgatt tccttgttct
     4381 gctccgcggt gttgctcgcc gcatcgagga tcggcttcgc actcggggtc gtccgcgaac
     4441 agctaccgag cgccagaatg atagcaatgg aggcgatggt tctcatggat cctcactact
     4501 ctacgacgac gacatcgcac ggaaagaccg cgtcgaggcc tgcatctccg agacgttgtc
     4561 ggcggtccaa agcgtcgcac gcgaatcgac gccttggccg gcgctggcga gctcgagctc
     4621 cccgcagaca gtggggacag cctggaggcg aatggcgccc tccggcggca tgctcgcgtg
     4681 gcaagcgagt accagagcgg ccattcccgc cgaaacgaaa agcgcacgaa tcatcgagca
     4741 tccccgcgga aagggtaaca ggcaggcgag acccgacaac tcgaggggtt aatgggcgac
     4801 tctcgtaaga cgaaacgcaa gcccgaacgc ccagaccgcc tggattgcgc ccggacccaa
     4861 actctggcac cgctcagggc cgtgaagctc ccatccgaca cgttgattgc gcc
//
LOCUS       DASRZF010000025         4310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4144318, whole genome shotgun sequence.
ACCESSION   DASRZF010000025 DASRZF010000000
VERSION     DASRZF010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4144318"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="VFG22_01215"
     CDS             <1..936
                     /locus_tag="VFG22_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414884.1"
                     /translation="VLQRSANWIMPRNRKFYSDAQRRRFRRFPWLIDLTRRIQGFLMG
                     QVQYAATIGHARMGQFEWFALRYLERAIEDPVLRAQLTPGSRYACKRGLVSDDFYPAL
                     TRDNVELVPSALKEVTEDGLVTADGRQIEIDVIIYCTGYRILDFDRIDVVGSSGLSLA
                     EAMGDTPKAYKGIATPGFPNYFFVIGPNGLVLHVPYFTTAERNVKTIVGLLKEMQRAG
                     LIAIDVRQSVTDEYFDWMTTQFGRFSWGSADCNSYYTNAAGHAPFLFPGNFTQHVQFH
                     EQIGLEDFKVVARTETRAHRSSKDRSWMTSQAKQR"
     gene            906..1754
                     /locus_tag="VFG22_01220"
     CDS             906..1754
                     /locus_tag="VFG22_01220"
                     /inference="COORDINATES: protein motif:HMM:NF004843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6414885.1"
                     /translation="MDDFSGKTAVITGAASGIGFAMAERFASEGMRVVLADIEQAALD
                     AAVARIVATGAEALGVLTDVAKAEDVAALAERARSEFGGIHIACNNAGVFAGGLLWEE
                     SLADYRWLMDVNVWGVIHGIRTFIPIMLEQDTECHLVNTASMAAVTVMPFAGIYHMTK
                     HAVLALSEALFHELAMSSEKVKVSVLCPELIKTGIATCERNRPAEYSKAGDLIESDAR
                     QLVMDAISDGVESGVEPSVMADRVVHAIRSGAFYILSEDGWRETANQRMEDIRTGSNP
                     VLSPPV"
     gene            1780..4242
                     /locus_tag="VFG22_01225"
     CDS             1780..4242
                     /locus_tag="VFG22_01225"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HET6414886.1"
                     /translation="MRSFQPRARLSCESPPSVPSAHLRKRQATVAALLLLAFTSGASA
                     QSVLPHPDPIFDGKIQRTLEGSKAGDAAPLRPPAGAPNVLIVLVDDAGFGNPSTFGGP
                     VQTPSLDALAEGGLRYNRFHVTGLCSPTRAALLSGRNHHSVGFGMVSEVHSDFPGYNA
                     HWPKSAASLARILRDNGYSTAAIGKWHLTPDDEQGAAGPFDRWPNAMGFDYFWGFLGG
                     EDSHYDSMIVENNSIVGVPTEKDFYLTTAMADQAIDWLRNQKTQSPERPFFLYFATGA
                     SHAPHHVAKHWADKYEGQFDEGWDALREATFARQKKLGVIPEDAKLTPRPDAIPAWDS
                     LSPEEQKLFARQMEVYAGFQECTDHEIGRVLGSLDAMGLSDDTLVIYIFGDNGASLEG
                     SLSGSFNEMTVLNGIPLTMEQQFEAIKAYGGIEVWGGPRTAPHYAAGWAWAGDTPFQW
                     GKQVASHLGGTRNPMVVSWPGRIKELGGLRSQFTHATDIAPTILEVAGLPVPDVVDGT
                     PQTPMHGVSFANSFSEPDAPEHHRQQYFETVGNRAMYKDGWIWSCRVPRIPWQLDPET
                     IARFAPGRWNPEDDPCELYDLENDFSQAEDLSSEHPDKMRELEALFWKEAESNQVLPL
                     FGGLATLWGMAPPGNPGPKRATFYPGVQNVAQGMIPQLYNRSFTITADLEIERNWCAF
                     SLCYGADGVIVANASFLGGFSLYVQGGKLRYTYSFLGLKVDDLIASERMPTGKVQVRY
                     EFTADKPGEMATGGTHRLFVAGKQVAEGKLEHTVPVRFSGHAGLDIGRDNGLPVSPNK
                     IYYLRAPFPFEGTIEQVVFEVQ"
BASE COUNT          842 a         1328 c         1359 g          781 t
ORIGIN      
        1 gtcctgcagc gctccgcaaa ctggatcatg ccgcgcaaca ggaagttcta cagcgatgcg
       61 cagcgacggc gatttcgaag gttcccctgg ttgatcgacc tcacgcggcg catccaaggg
      121 ttcctgatgg ggcaggtgca gtatgcggcg acgatcggac atgcgcggat gggccagttc
      181 gaatggtttg cgctgaggta cctcgagcgc gccatcgaag accccgtgct ccgggcgcaa
      241 ctcacgcccg gcagccgcta cgcgtgcaaa cgcggcctgg tctcagacga tttctacccc
      301 gcgctgactc gggacaacgt cgagctcgtg ccgtcggcac tcaaagaagt caccgaagac
      361 ggcctcgtca ctgcggatgg gcggcagatc gagatcgacg tcatcatcta ctgcacgggt
      421 tatcgaattc tcgattttga tcgcatcgac gtcgtaggtt cgagcggact gtctctcgcc
      481 gaggccatgg gagatacgcc caaagcctac aagggcatcg cgacaccggg cttccccaac
      541 tacttcttcg tgataggtcc caatgggctc gtgctccacg tgccttactt cacgacggcg
      601 gagcgcaacg tgaagacgat tgtcggcttg ctgaaggaga tgcagcgggc gggtttgata
      661 gcgatcgacg tccgccagag tgtgacagac gaatacttcg actggatgac aacgcagttc
      721 ggacggttct cctgggggtc ggcagactgc aacagttact acacgaatgc agcaggccat
      781 gcgcccttcc tgtttccggg caacttcact caacatgttc agtttcacga gcaaataggg
      841 ctagaggatt tcaaggtcgt cgcgaggacc gagacccgag ctcatcgatc aagtaaggac
      901 cgatcatgga tgacttctca ggcaaaacag cggtaatcac tggtgcagcg agcggcatcg
      961 ggtttgcgat ggccgagcgc ttcgcctccg agggcatgcg cgtggtcctc gccgacatcg
     1021 aacaggcagc tctcgacgcg gctgtggcgc gcatcgtcgc gacaggcgca gaggcgcttg
     1081 gagtcctgac cgacgtggcc aaagccgagg atgtcgctgc gctggcagag cgcgcccgat
     1141 cggagttcgg gggcatccac atcgcctgca acaatgccgg agtctttgcg ggcggcctgc
     1201 tctgggagga aagcctcgct gactatcgct ggttgatgga tgtcaacgtc tggggtgtga
     1261 tccatggtat cagaacgttc atcccgatca tgctcgagca ggacaccgag tgtcatctcg
     1321 tgaacaccgc gtcgatggct gccgtcacgg tgatgccgtt cgccgggatt tatcacatga
     1381 cgaagcacgc cgttttggcg ctcagcgaag cgctttttca cgagctcgcg atgagctccg
     1441 agaaggtcaa ggtgtcggtt ctctgcccgg agctgatcaa gaccggcatt gcgacctgcg
     1501 agcgaaaccg gccggccgaa tattcaaagg caggggatct catcgagagc gacgcccggc
     1561 agctggtgat ggacgccatc agcgacggtg tcgaaagcgg ggtagaaccc tcggtcatgg
     1621 ccgatcgagt cgtccacgcg atacgctctg gcgcgttcta catcctctcc gaggatggtt
     1681 ggcgcgagac cgcgaaccag cggatggaag acattcgcac cggaagcaac ccagtccttt
     1741 cgccgcccgt ttgaccgtct atctctccgg agccctccaa tgcgatcttt ccagccacga
     1801 gcaagacttt cttgcgaatc cccgccttcg gtcccgagcg cgcatctgag gaaacggcag
     1861 gcgaccgttg cggcgctgct gctcctcgca ttcacctcgg gagccagcgc gcagagcgta
     1921 ctgcctcatc cggatcccat atttgacggt aagatccagc gcaccctcga agggtcgaag
     1981 gctggtgacg cagcgccgct gcgacctccg gctggcgcac cgaacgtgct catcgtgctc
     2041 gtcgacgacg ctggcttcgg caacccctcg accttcggag ggccagttca gacaccaagc
     2101 ctcgacgccc tcgccgaggg cggcctgcgg tacaatcgct tccacgtcac ggggctctgc
     2161 tcccccacgc gagcggccct gctctcgggc cgcaaccatc acagcgtcgg tttcggaatg
     2221 gtctctgagg tgcacagtga cttccccggc tacaacgcgc attggccgaa gagcgcggcg
     2281 tcactcgcaa ggatcctgcg cgacaacggc tacagcaccg cggcgattgg aaagtggcac
     2341 ctcacccctg acgacgaaca gggcgccgcc ggacccttcg accgctggcc aaacgcgatg
     2401 gggtttgatt acttctgggg ttttctcggc ggtgaggatt cgcactacga ctcgatgatc
     2461 gtcgagaaca actcgatcgt gggtgtcccg acggagaagg atttctacct caccacggcg
     2521 atggccgacc aggcgatcga ctggttgcgg aatcaaaaga cgcagtctcc cgagcgaccg
     2581 ttctttctct acttcgcgac tggcgcgagc cacgcgccgc accacgtggc caagcattgg
     2641 gccgacaagt acgagggcca gttcgacgag ggctgggacg cgctgcgcga ggccactttc
     2701 gcacgccaga agaaactcgg cgtgatcccc gaagacgcaa agctgacgcc gcgccccgat
     2761 gcgattcccg catgggattc gctgtcacct gaagagcaga agctctttgc ccggcagatg
     2821 gaggtctacg cggggtttca ggagtgcacg gaccacgaaa tcggtcgcgt cctcgggagc
     2881 ctcgatgcga tggggctctc ggacgacacg ctggtgattt acatcttcgg tgacaatggg
     2941 gcgagcttgg aaggtagtct cagcggctcg ttcaacgaga tgaccgtgct caacggcatc
     3001 cctctcacca tggagcagca gttcgaggcg ataaaggctt atggcggcat cgaggtttgg
     3061 ggcggtccgc gaaccgctcc ccattatgcg gcgggttggg cctgggcggg cgacaccccg
     3121 ttccaatggg gcaagcaggt agcgtcccat cttgggggaa cgaggaaccc gatggtcgtg
     3181 tcctggccgg ggcgcatcaa ggagctcggc ggcctgcgga gccagttcac ccacgcgacg
     3241 gatatcgctc ctacgatcct cgaagtggcc gggctcccgg taccggacgt cgtcgacggc
     3301 acgccgcaga cgcccatgca cggcgtgagc ttcgcgaaca gcttttcgga gccggacgct
     3361 cccgaacatc acaggcagca gtacttcgag acggtgggca accgcgcgat gtacaaagac
     3421 ggttggatct ggagctgccg cgtcccgcgc atcccgtggc agttggaccc ggagaccatc
     3481 gcgcgcttcg cgcccggacg ctggaacccg gaggacgatc cttgcgagct ctacgatctc
     3541 gagaacgact tctcgcaggc cgaagacctt tcgtcggagc atccggacaa gatgcgagag
     3601 ttggaagcgc tgttctggaa ggaagcggag agcaaccagg tgctgccgct gtttggcggt
     3661 ctcgcaacgc tctggggcat ggcgccgccc ggaaacccgg gaccgaagcg agccacgttc
     3721 taccctggcg tgcagaacgt cgcgcagggc atgattccac agctctacaa tcgttccttc
     3781 acgattaccg ctgacctcga gatcgaacgc aactggtgtg cgttctcgct ctgctacggc
     3841 gccgacgggg tcatcgtggc gaacgcgagc ttcctcggcg ggttttcact ctacgtccaa
     3901 ggcgggaagc tccgatacac gtacagcttt ctcggactga aggtcgacga cctgatcgca
     3961 tccgagcgca tgcccacggg caaggtgcag gtgcggtacg aattcactgc agacaagccc
     4021 ggcgagatgg cgacgggcgg gacgcaccga ctgttcgtcg ccggcaaaca agtcgcagag
     4081 ggcaagctcg aacacaccgt tccggttcgc ttctcggggc atgccgggct cgacatcgga
     4141 cgcgacaacg gattgccggt ctcgcccaac aagatctact acctccgcgc gcccttcccg
     4201 ttcgagggga ctatcgagca ggtggtgttc gaggtgcaat gaaccggctt ttcgaacgca
     4261 cgctggatgg cagggcgagc gtcgagtcgc tcgaaccagg cttgcagatg
//
LOCUS       DASRZF010000026         7912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3801340, whole genome shotgun sequence.
ACCESSION   DASRZF010000026 DASRZF010000000
VERSION     DASRZF010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 7912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7912
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3801340"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1545
                     /locus_tag="VFG22_01230"
     CDS             <1..1545
                     /locus_tag="VFG22_01230"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HET6414887.1"
                     /translation="QVPVAEDLSSFLGFLSKPDFSLWANPALYSAALTVAIVASLETL
                     LNLEAVDNLDPKQRHSPPSRELIAQGVGNIACGFLGGLPVTSVIVRSSVNIGAKVETK
                     LSTIFHGVLLLVSVMLLPGWLNMIPLSCLAAILLVTGFKLASPTLFKRMWSEGRTQFA
                     PFAVTVAAIVLTDLLVGVLIGLASAIGFILHSNFRRPLRKIVEKHLGGEVVHIELADQ
                     VSFLNRGALSRELDAVQRGSHVLLDARTTDYIDPDVLDLIHDYKDKAAPARGVELSLV
                     GFHSKYSELQDRTQYVDYSTRELQDAATPEQVLQILKDGHERFRSGKRLTRDLARQVG
                     ATAEGQHPLAVVLSCIDSRTPAELVFDLGMGDIFSARVAGNVAGRKVLGSIEYACAVA
                     GAKLILVMGHTRCGAVTAAVRLTESGDEPEQATGCQHIGHIVRDIEASVDIERVKGLG
                     ELPKGAQEALIDEVARRHVARVVQTLPIESRTLGALLRDGEIAIVGAMYDVVTGDMEF
                     LEDSEQ"
     gene            1545..3053
                     /locus_tag="VFG22_01235"
     CDS             1545..3053
                     /locus_tag="VFG22_01235"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET6414888.1"
                     /translation="MAANASTLQLAGLLTLGALCLWPQSGHTQDQPSIASSVVSFNVL
                     SDAPDLTIPWQTTGIENFGGSGVIIDGRRILTNAHVVESAVSVEVKRADGSEPFPAKV
                     AFISHDADLALVEVEDPRFFDGANPLPIGEMPKLQQPVLVYGFPIGGLTLSITSGIVS
                     RVEIDIYAQSNRRLLSVQIDAALNEGNSGGPVVTDGAIVGIAMQGFDGAENVGYMVPS
                     PVIRHFLADVADGRYDGFPQLGIDVQDMESQSQRRATGMTANQSGALVTRVDFDGPAY
                     GTLRPRDVLLSVDGHAISNDLTVSWPGIGRVDYELAFQSKQIGESISVTFLRHGKTLN
                     KNIELAPHTQLVPGRRTTEWPRYFQFGGLVFQPLSDEMLDAPGARDEAYPDSYSYSEV
                     NNLVTKERREIILLGQVLPHPVNRGYQDWGGETIRLVNGIIPRDLQHLAAIVDGASGP
                     WLRIVTGDGWLLTLDLEAARKANSDILMDYGVVHDRYLGTDLPEARKRRGRR"
     gene            3209..7111
                     /locus_tag="VFG22_01240"
     CDS             3209..7111
                     /locus_tag="VFG22_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HET6414889.1"
                     /translation="MSTGNPRSIPITADQSALWLGGSSTANQRSWAFTIRGALSVTAL
                     REALTALSARHESLRSSFAPGGKQLRLWPPTNVTLRMFTLSPTSGAEEWAVFVRDTVD
                     IEQREPFDLTSGPMFRAGLCSDGARRHCLILSAHLLCADQVSWSVLASDLGTLYAEAL
                     RGTSGLDQGSSALPHAPSFVANNERIFEAQTAGADSSARYWKEQFTSAPSPLDLPIDH
                     PRTTKRVRSAATLVRELSPDTQRRLSEFSAHSDVDAAQTCLAATFVLLSRLTGQDDIV
                     VVTKSEQLSAGARANSVGSCRPLYPLRLKLQSRDCFADVVRRVADAYRQVESHPHVDP
                     FGLFAQFGGVHDATRRAISTVFFGSYPEPRLSLSGLETETHPAQPAETDFEWKFALVR
                     KPTGLLFSLTYNLQLFDEAQAQLRLAEFETLLVSALTEPSRSVSELSLLTKEEEELLA
                     EVGVGPVDLRPDVPAHQLIERSVARFPEATALEFQGKTLTYAAMDARANALAHALVER
                     GVVAGDLVAVILRRGLDLPIALQAIWKVGAAYVPIDPDYPASRIRMILKDAAPRVIVT
                     ESRVESDALSAFQPALVRLNELSLTPRSSAPQALAQPDALAYVIFTSGSTGRPKGVRI
                     RHSAFSNFLQSMAKAPGMRPGDKLLAVTTICFDIAGLEMFLPLIVGGTTEIVSRNVAV
                     DGLELKSKLESGQYALFQATPATYRMLFEAGWNGTETLKVLCGGEAFPLDLAARLVSG
                     CKEVWNMYGPTETTVWSTLHRVTAEDTEFVPIGRPIHHTQIVLLDAAGERVPLGVPGA
                     LYIGGAGLAAGYHLRDDLTQSAFVDSRHATDPREPRLYRTGDLARYHVDGRLECLGRT
                     DYQVKIRGFRIELGEIEHHLQEFEGVRDAVVIAREYESGDKRLVAYLTWKAKPPECAE
                     QIQAHLARRLPSHMIPTAFVSLDQLPLSPARKVDRKRLPEPESGARLDPVGLASPTTD
                     TEVRVAQLFARALDVPRVGIHDHFVDAGGHSIRAIGLLKDLSTEFGSEFDLAMLNEAP
                     TVAEMAALIDAGGWGDRASPVVKLYSGDGSAPLFLLRGVYLYQELARTLNSAGDVYAV
                     YLPEETDILRDDDGKLVPDGTHKLAAAYIRAIKKRRPKGPYHIAGMSYGGVIGYEIAQ
                     QLTQAGEEVGFLGLFDPVLSRRHHPFPARWLLRKGQQTLQRLTSKRPRAYVLGSDFTP
                     SAPTSAPQRMSSKQLMRVIEVYAKSMRPYAGNLDLFVAQDRQDQLRYLIPADCGWGRW
                     IRGRFSVTPVPGDHLTMFTKPHVVSLAAAVNERLAPVL"
     gene            7342..>7912
                     /locus_tag="VFG22_01245"
     CDS             7342..>7912
                     /locus_tag="VFG22_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414890.1"
                     /translation="MRLVLIGVLAGALGLVGCGDDATSGGGTGGTAGTGGTAGTGGTG
                     GGGGPEACGAGESLGEGFATDEGSVTCDGLGVITVPISVVLAANADVVEGETDVDVQM
                     QFIISEDTVAELGALVQEALIGESSADVDDSEGGGLVNVPATVPCTVDFAADPDDNSI
                     PGPIVVTTPVVVATWTAVDGSIVIEAVDMT"
BASE COUNT         1529 a         2328 c         2484 g         1571 t
ORIGIN      
        1 caggtgcccg tcgccgagga cttgtcgagc ttcctcgggt tcctcagcaa acccgatttc
       61 tctctctggg caaacccagc gctctactca gcagcgctga cggtcgcaat cgtggcgtcc
      121 ctcgaaacac tgctcaacct cgaagcggtt gacaatctcg accccaagca gcgccactcg
      181 ccaccgagcc gcgagttgat cgcgcaaggg gtcggaaaca ttgcatgcgg ctttttgggc
      241 gggctgccgg tcacgtcggt gatcgtgcgc agctcggtca acatcggcgc gaaggtcgaa
      301 acgaagctct cgaccatctt ccacggcgtg ctcttgctcg tgagcgtgat gttgttgcca
      361 ggttggctga acatgattcc cctttcgtgc ctggccgcga tccttctcgt tacgggattc
      421 aagttggcga gccccaccct attcaagcga atgtggtcgg aaggtcgcac gcagttcgcg
      481 cccttcgcgg tgacggtggc agcgatcgtg ctgacggact tgctggtcgg tgtcttgatc
      541 ggcctcgctt ccgcgatcgg gttcattctg catagcaact ttcggcgccc acttcgaaag
      601 atcgtcgaaa agcacctcgg gggagaagtg gtccacatcg agctcgcgga tcaggtgagc
      661 ttcctcaatc gaggggcgct ctcgcgtgag ctcgacgccg tccaacgagg cagtcacgtg
      721 cttctggatg cgcgaacgac cgactacatc gatccggacg tgctcgactt gattcacgat
      781 tacaaggaca aggccgcacc cgcgcgcgga gtcgagctga gcctagtcgg gttccattcc
      841 aagtactccg aattgcagga ccgaacccag tacgtcgact actctacccg cgagctgcag
      901 gacgcggcca caccggagca ggtgctgcaa atcctcaagg atgggcacga acgcttcagg
      961 agcggcaagc gtctgacgcg ggatctcgcg cgacaggtcg gtgcgacggc cgagggacag
     1021 catccccttg cagtcgtgct gagctgcatc gattcgcgga cgcccgcaga gctggtcttc
     1081 gacctaggca tgggcgacat cttcagtgcc cgggtcgcag gcaacgtggc gggcaggaaa
     1141 gtactgggga gcatcgaata cgcttgcgcg gtcgccggcg ccaagctgat cctggtgatg
     1201 ggtcatacgc gctgcggagc cgtgacggcg gcggtccggc tcacggagag cggagacgaa
     1261 ccggagcagg cgaccggttg tcagcacatc gggcacatcg tccgtgacat cgaggcctcg
     1321 gtggacatcg agcgagtgaa gggcctcggg gagctcccca agggggctca ggaggcgctc
     1381 atcgacgagg tggcgcgccg gcacgtcgcg cgtgtagtgc aaacgcttcc gattgagagc
     1441 cggaccttgg gcgcactcct tcgcgacggc gagattgcca tcgtcggtgc gatgtatgac
     1501 gtcgtgaccg gagacatgga gttcctcgaa gattcggagc aataatggct gcaaatgcga
     1561 gcactcttca actcgcgggg ctgttgaccc tcggcgccct atgcctctgg cctcagagtg
     1621 gacataccca ggaccagccc tcgatcgcgt ccagcgtggt gagcttcaat gtgctgagcg
     1681 acgctccgga cttgacgatc ccctggcaaa ccacgggtat cgagaacttc ggcggctcgg
     1741 gcgtgatcat cgacggacgg cgtattctca cgaatgcgca cgtggtcgaa agcgcggtga
     1801 gcgtcgaagt caagcgcgcc gacggaagtg agccgttccc cgcgaaagtc gctttcatca
     1861 gccacgatgc agacctggcg ctggtcgaag tcgaagaccc gcggttcttc gacggcgcaa
     1921 acccgctgcc gatcggcgag atgcccaagc ttcagcagcc ggttttggtg tacggctttc
     1981 caattggtgg gctcaccttg tcgatcacct ccgggatcgt ctcgcgggta gagatcgata
     2041 tctatgcgca atcgaaccgc cgcctgctct cggtacagat cgatgccgct ctcaacgagg
     2101 gaaacagcgg tggacctgtc gtgaccgacg gcgccatcgt cggtatcgcc atgcagggtt
     2161 tcgatggcgc tgagaacgtc ggctacatgg ttccgagtcc cgtgatacgg cacttcctcg
     2221 ccgacgtggc ggacggccgc tacgacggtt tcccgcaact gggaatcgat gtgcaggaca
     2281 tggaaagcca gtcgcagcga cgtgcaactg gcatgacggc caatcagagc ggcgcgctgg
     2341 tgacccgcgt cgacttcgat gggcctgcat acggcacact cagaccgcgc gacgttctgc
     2401 tcagcgtcga cgggcacgcg atctccaacg acctcacggt ttcctggccg ggtatcgggc
     2461 gcgtggacta cgagctggca ttccaaagca aacagatcgg cgaaagcatc agcgtgacgt
     2521 tcttgcggca cggaaagaca ctgaataaga acatcgagct cgcgccacat acccagttgg
     2581 ttcccggtcg cagaacgacc gaatggcccc ggtacttcca attcggaggg ttggtctttc
     2641 agccactcag cgacgaaatg ctcgatgcgc ccggagcccg ggacgaggcc tatcctgatt
     2701 cgtacagcta ctccgaggtg aacaacctcg tgaccaagga acgtcgcgag atcatcttgc
     2761 tcgggcaggt gttgccccat ccggtcaatc gcggctacca agactggggt ggcgaaacga
     2821 ttcgactcgt caacggcatc atcccgagag acctgcagca cctcgccgcc atcgtcgatg
     2881 gcgccagcgg cccctggctt cgcatcgtga cgggagacgg ttggctgctg accctcgacc
     2941 tcgaagccgc ccgaaaagca aattccgata ttctcatgga ctatggcgtt gtgcatgatc
     3001 gctatctcgg taccgatctt cccgaggcgc ggaagcgtcg cggacgccgt tagcgacgag
     3061 cgcgtcgaca cgcttccaag aatcgaaggg ccagcaccgg cgagctgcca attctcccgg
     3121 atgagatccg ggtatttggt gagattttgc gccggaaacc aaggccgaca cacttgctct
     3181 cattggtgtg ccgggacaca ctggctcggt gtcgaccgga aatcctcgca gtattccaat
     3241 cacggccgac cagagtgcac tttggctagg tggaagtagc accgcgaacc agcgatcatg
     3301 ggcattcacg attcgcggcg cgctgagcgt cactgcgctg cgagaggcac tgacggcgct
     3361 ttcagcgcgg catgaatccc tgcgctcgtc gttcgcacca ggtggcaaac agctcaggct
     3421 ttggcctcca acgaatgtga cgctacgaat gttcacgctg agcccgacgt ctggtgctga
     3481 agaatgggcc gtattcgtga gagatacagt cgacatcgag caacgcgagc cgttcgactt
     3541 gacgagcggc ccaatgtttc gggctgggtt gtgctccgat ggcgcaaggc gccactgctt
     3601 gatcttgtcg gcacatctgc tttgcgcaga ccaagtttct tggagtgtgc tggcgtcaga
     3661 cttgggtaca ctctatgcgg aggcgcttcg cggcacgagc gggctcgatc aaggatcttc
     3721 cgcattgccc catgctcctt ccttcgtggc gaacaacgag cgcatattcg aagcgcaaac
     3781 cgctggcgca gattcctctg ctcgctactg gaaagagcaa ttcacctccg cgccgtcgcc
     3841 cctggatttg ccaattgatc atccccgcac gaccaagcgc gtgcgaagcg cggcgaccct
     3901 cgtgcgtgag ctgtcgccag acacgcagcg ccgcttatcg gaattttcgg cacactcgga
     3961 tgtggacgct gctcagacgt gcctagctgc cactttcgtc ctgctcagtc gtttgaccgg
     4021 acaggacgac atcgtggtgg tcacgaagtc tgagcagctg agcgctgggg ctcgggccaa
     4081 ttccgtaggc agttgccggc cgctctatcc actgcgtttg aagctgcagt ctcgggactg
     4141 cttcgcagat gtcgtcaggc gggtcgccga cgcatacagg caagtcgagt cccaccctca
     4201 cgtcgatcct ttcggattgt ttgcccaatt cggcggtgtt catgacgcta cgcggcgtgc
     4261 catctctacg gtgttcttcg ggagctaccc agagccgcgt ttgagtctct cgggtttaga
     4321 gaccgaaacg cacccggcac agcctgcaga aacagacttc gaatggaagt ttgctttggt
     4381 ccgaaaacca acggggctgc tgttttccct gacctacaat ttgcagctct tcgatgaggc
     4441 tcaagcgcag ctgcggttgg ccgaatttga aacgctgttg gtgagcgcac tcaccgaacc
     4501 ctcccgttcc gtctccgagc tttcgttgct cacgaaggaa gaagaggagc tccttgccga
     4561 ggtcggtgtt gggccggtag atctgcgccc ggacgtgcct gcgcaccaat tgattgagcg
     4621 ctccgtggca cgctttccag aagcgacggc actcgaattt caggggaaga ccttgacgta
     4681 tgcggcgatg gacgcgcggg cgaatgcgtt ggcgcacgca ttggtggagc gcggcgtggt
     4741 cgcaggagat ctcgtggcag tgatcttgag gcgagggttg gatcttccga tcgctttgca
     4801 ggcaatctgg aaagtcggcg ccgcgtatgt tcccatagac ccagactatc ccgcttcgcg
     4861 gatccggatg atccttaaag acgctgcgcc ccgcgtgatc gtgaccgaga gtcgcgtcga
     4921 gtccgacgcg ctttccgcct ttcaaccggc gttggtgcgt ttgaatgagc tttccctcac
     4981 accgcgatcg agtgcgccgc aggcgttggc gcagcccgac gctcttgcgt acgtgatctt
     5041 caccagcggc tctaccggac gcccaaaggg agtgcggatt cgacattctg cattttcgaa
     5101 cttcttgcag tccatggcga aagcgccggg aatgaggccg ggcgacaagc tgctcgccgt
     5161 gacgacaata tgcttcgata ttgccggctt agagatgttt ctgcctttga ttgtcggcgg
     5221 caccacagaa atagtctcac gaaacgtcgc agtcgacggg ctggagctga agtccaagtt
     5281 ggagtccgga caatacgctt tgtttcaagc tacccccgca acgtatcgga tgttgttcga
     5341 agcaggttgg aacggaactg agacactcaa ggtactctgc ggaggcgaag cgttcccgct
     5401 cgacctcgca gcgcgcctcg tcagcggctg caaagaagtc tggaacatgt atggcccaac
     5461 ggagacgacc gtttggtcca cgctgcatcg cgtgaccgct gaagacacgg agttcgtccc
     5521 catagggcgt ccgattcatc acacgcagat cgtgctcttg gatgctgcag gcgagcgtgt
     5581 gcctctgggc gtgcccggcg ctctgtacat cggcggtgca gggctagcag caggatacca
     5641 tctgagggac gacctcacgc aaagcgcatt cgtagatagc cgacacgcaa cggacccgcg
     5701 agagccgcgt ctctaccgca ccggagatct cgcgcgctat cacgtagatg gtcggctaga
     5761 gtgcctgggc cgcacggatt accaagtcaa gatccgtggg tttcgaatcg aactgggcga
     5821 gatcgaacac catcttcagg agttcgaggg cgtcagagat gcggtggtga tcgcacgtga
     5881 atacgaatcc ggagacaaac gcttggtggc gtatttgact tggaaagcaa agccgccgga
     5941 gtgcgctgag cagatccagg ctcatcttgc acgacggttg ccatcgcaca tgatcccgac
     6001 cgcattcgtt tcgttggacc aactgccgct ttcgccggcg cgcaaggtgg accgaaaacg
     6061 attgcctgag cccgagtccg gggcgcgact agacccggtg gggcttgcgt caccaacgac
     6121 cgacacggag gttcgcgtcg cgcaattgtt tgctcgggcg ctcgatgttc cgcgcgtcgg
     6181 cattcacgac cacttcgtag acgccggagg tcactcgatt cgagcgatag gactactcaa
     6241 agatctttcg acggagtttg gaagcgaatt cgatctagcg atgttgaacg aggcgccgac
     6301 cgtcgcggaa atggctgcgc taatcgacgc aggtggttgg ggcgatcggg cgagcccagt
     6361 ggtgaagttg tactctggcg acggctctgc accgctattt ttgcttcgcg gagtttatct
     6421 atatcaggag ttggctcgga ctttgaattc agccggagac gtgtacgcgg tctacctacc
     6481 ggaagagacc gacatcctac gggatgacga tggcaagttg gtgccagacg gaacccacaa
     6541 gcttgctgcc gcgtacatcc gcgcaatcaa gaagaggagg ccgaaagggc cctatcacat
     6601 cgccggaatg tcatatggcg gcgttattgg ctacgagatc gcccagcagc tcacccaagc
     6661 cggcgaggag gtcgggttct tgggcttgtt cgacccggtg ctctcgcgcc ggcatcatcc
     6721 gtttcctgcc cggtggttgc ttcgcaaagg acagcagaca ctgcagcgcc tcacaagcaa
     6781 gcgacctcgg gcgtacgttc ttgggagcga ttttacgccc agcgccccga cgtcagcgcc
     6841 gcagcgcatg agcagtaaac aactgatgcg cgtcatcgaa gtgtacgcaa agagcatgag
     6901 gccgtatgcg ggtaacttgg acttgtttgt cgcccaagac cggcaggacc aattgcgcta
     6961 tttgataccc gccgactgtg gctggggccg ttggattcgt ggacggttct ctgtaacccc
     7021 ggttccgggt gaccacttga cgatgttcac gaagccgcac gtcgtctctc tcgcggcggc
     7081 ggtcaacgag cggctcgcac cggtcttata gacagcgaac tgcaattctg agcagcctcc
     7141 tcgagggcca tggaactgac tgcgccaaga tgactatggc gccgatttcg atgggaaagg
     7201 agctcgcgta gacgtggtcc agttgccttc ctggacgact ccagatacac gggtcgggcg
     7261 cgtgttgggc gcaatgggct cgtctgtgcc aagcttcgca ctgaccgggc gtttctagga
     7321 gaatagaagg atatgaaatt catgagactt gttttgattg gcgtgctcgc gggcgcgctc
     7381 ggtttagttg gttgcggcga tgacgcgact tcgggtggtg gcaccggtgg aacggctggc
     7441 acgggcggca cggctggcac gggcggcacg ggcggcggcg gcggccccga ggcttgtggc
     7501 gccggcgaat cgctcggcga gggtttcgca acggacgagg gctcggtgac ttgtgacgga
     7561 cttggcgtca tcaccgttcc catcagcgtc gtgcttgcgg ccaatgccga cgtcgtagaa
     7621 ggcgagacgg atgtcgatgt acagatgcag ttcatcattt cggaggacac cgtcgctgag
     7681 ctcggggctt tggtgcaaga ggcgctgatc ggcgagtcgt ccgcggatgt cgacgacagc
     7741 gaaggcgggg gactggtgaa tgtgccggca accgttcctt gcacggtcga cttcgccgca
     7801 gacccggacg acaactcaat cccgggtccc atcgtcgtca ccacgccagt cgtggtggca
     7861 acctggaccg cggtcgatgg atcgatcgtc atcgaggccg tcgacatgac gt
//
LOCUS       DASRZF010000027         4620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4490751, whole genome shotgun sequence.
ACCESSION   DASRZF010000027 DASRZF010000000
VERSION     DASRZF010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 4620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4620
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4490751"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VFG22_01250"
     CDS             complement(<1..378)
                     /locus_tag="VFG22_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414891.1"
                     /translation="MEAGSHDSLADQLLELQTIHEARVAAEEEAKLKAAEDARRWAEL
                     EAKRRREEEEARRRSEEEARLAEEQAQRAEVERRAREAKEAELRVHLQEEAKARAAEQ
                     QRMHAHEKELATITAVEKAKIRSH"
     gene            complement(540..1907)
                     /locus_tag="VFG22_01255"
     CDS             complement(540..1907)
                     /locus_tag="VFG22_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414892.1"
                     /translation="MHRIALTVLLGALLTTATARAGNSDEVNGGLDVTLTGGAVVAIT
                     YTGAALWYNPAGIAQINKASLELTGVTMQIQILKVPGLLTIDTDPQAKSEGKTVSFTV
                     VPQAVTFTIGLRENLKLGVGLFNSSIRRAFVTEQVKTASGILPEARAVGGQDSKIDFF
                     HISGGLAGTFGQKQKVLVGGAFDFVVAKARTDRTQSIFYDGGVAGFSTLGDVATETGF
                     GVQIKGGIQWVPIPKVRIGLSIASPTFAFVIIERFANNLGQSPPVGTTLPSGDPNAQT
                     SVGAESRGTSGGWWGVEPGNLRFGIAYLGDWGWIEADLVAQWRLRTAELDIDMQATVN
                     GRIGSAFRLTKHVHLGLGLFTDRSQIDELQVAPFATTDVNFYGAQLGFLFSNREVHPG
                     RPSEAQSQKGGFAVAFGFRYSHGRGQALGVLFPAQYDPSSITREGTSGKINEIGLNIG
                     ANVSF"
     gene            complement(1979..2995)
                     /locus_tag="VFG22_01260"
     CDS             complement(1979..2995)
                     /locus_tag="VFG22_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414893.1"
                     /translation="MVNMARDDVTVAQLRAMDYQYATRFGESVAMCVMQIVAQTEDPY
                     IKARAYEWRMWAMPQARAAAFDQDPVAGLLELWVLAGQQRQYFTEGEGTRYFGELPGC
                     VSETTKQLEEQAEQLAYTVMNDKERRELPDDVRAWIADHPIEGQLMVRPTARADLAGL
                     VSEAKQGGLQAVGSMEETFRDLNDRLAILTVQLPVEARWQADYLVQALFEERIQEPAD
                     AVVATAEKFTGFLDDFESTMDGQVSTLLDAIARERREIFEAVAVERAAILEAIGQERI
                     SAMSMLDEELIAATAKLDTVGRGLIDHFFVRLLEVLAVVGVFTFLIVALVLSVVRRRE
                     RNDD"
     gene            3344..>4620
                     /locus_tag="VFG22_01265"
     CDS             3344..>4620
                     /locus_tag="VFG22_01265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HET6414894.1"
                     /translation="MTEENYGLGTRALHAGQEPDPTTNSRAVPIYATTSYVFNDTDHA
                     AALFGLAEFGNIYSRLMNPTVDVLEKRLAALDGGVTGLCFASGQSAINAAILTLCHSG
                     QNFVSATSLYGGTWTLFNNTFKNLGVEVRFFDPAHPEQIHELVDENTRLVYIESIGNP
                     KNDVPDFKAIAEAAHSAPHGALPLICDNTVMTPYLFRPFEHGIDINVYSTTKFIGGHG
                     THIGGAIVDSGKFQWTENREKWPEFCGPSPNYHGAVLADALAPMGNIVYNVQIRTHWL
                     RDTGAAMSPFAAFLFLQGLETLHLRMPRHCENAQAVAEHLEKHPAVQWVNYPGLPSHP
                     DNERANQYLPDGKGAILGFGIKGGMEAGKKFINSVKLCSHLANIGDAKTLVIHPASTT
                     HQQLSPAEQQQAGVVPEYVRVSVGIEDVKDIIA"
BASE COUNT          877 a         1527 c         1321 g          895 t
ORIGIN      
        1 atgcgatcgg atcttcgcct tctcgacggc ggtgatcgtc gcgagctcct tttcatgcgc
       61 gtgcatgcgt tgctgttcgg cagcgcgcgc cttggcctct tcttgcaggt gaacacgaag
      121 ctcggcctcc ttggcttctc gtgcacgccg ttccacttct gcacgctgag cctgttcttc
      181 ggccaggcgt gcttcttctt cgctgcggcg acgggcttct tcttcttctc gacggcgctt
      241 ggcctcgagc tccgcccagc gccgtgcgtc ctcggctgct ttcagcttgg cctcttcctc
      301 ggccgcaacg cgcgcctcat gaatcgtctg cagctccaag agctggtccg ccaatgaatc
      361 gtggctccct gcttccattc ctgtcccccg cctcatcaaa gagtctggca aatcggcagg
      421 gcctttgctt gcgagttttc gcttcatgcc ccgcaaacac gcgcgaggcg cggcttggga
      481 tctatcctgt gagctgcatt accgacttga gccccatttt cgtgcggttc tgagctcgtt
      541 cagaagctga cgttcgcacc gatattgaga ccgatttcgt tgatcttgcc gctggtgccc
      601 tctcgcgtga tcgacgatgg gtcatactga gcgggaaaaa ggacgcctag cgcctgcccg
      661 cgtccatgcg agtagcgaaa gccgaaagcg acggcgaagc cacccttctg gctctgagcc
      721 tcactaggcc gaccagggtg cacctcccga ttggaaaaca gaaaacccag ttgcgcacca
      781 tagaaattga catcggtcgt cgcgaatgga gccacctgga gctcgtcgat ctggctccga
      841 tcggtgaaca gccccaagcc gaggtggaca tgcttcgtca gtcgaaaggc gcttccgatg
      901 cggccgttga ccgtggcctg catatcgatg tcgagctccg cggtgcgcag acgccactga
      961 gccaccagat ccgcctcgat ccagccccag tccccgagat acgcgattcc gaaacgcaag
     1021 ttgcctggct cgacgcccca ccagcctccg cttgtcccgc gggactccgc accgactgag
     1081 gtttgtgcgt tgggatcgcc cgacggaagg gttgtcccaa cgggcgggct ctgccccaag
     1141 ttgtttgcga agcgctcgat gatcacgaac gcaaacgtgg gcgatgcgat cgacaatccg
     1201 atccggactt tcgggatcgg gacccattgg atgccgcctt tgatctgcac gccaaaaccg
     1261 gtctcagtgg cgacgtcgcc aagcgtggag aagccagcca ctcccccgtc gtagaagatc
     1321 gactgcgttc gatcggtccg cgccttggca acgacgaagt caaacgcgcc gccgaccagg
     1381 actttctgct tctggccaaa cgtcccagct aaaccgccgc tgatgtggaa gaagtcgatc
     1441 ttggagtcct ggccaccgac cgcgcgcgcc tccgggagga ttccggatgc ggtcttgacc
     1501 tgctcggtga cgaacgcgcg gcgaatggag ctgttgaaaa ggcccacgcc gagcttgagg
     1561 ttctccctga gcccgatcgt gaatgtcacc gcttgaggga cgaccgtgaa gctcaccgtc
     1621 ttgccctcgc tcttcgcctg agggtcggtg tcgatcgtca gaagcccggg gaccttcagg
     1681 atctgtatct gcatcgtcac acccgtgagc tcgagggagg ccttgttgat ctgcgcgatg
     1741 cccgctgggt tgtaccaaag tgccgcaccg gtataggtaa tcgccacgac agcgccgcca
     1801 gtgagggtga catcgagacc gccgttcact tcgtcggagt tcccggcgcg cgcggtggcg
     1861 gtcgtcagca atgcacccag gagcacggtc aacgcgattc ggtgcatgga cgaatcctat
     1921 aaggaatcga tcatgagtat caaaattagc gtcttcgaac gagaggcggt cgaccctgtc
     1981 agtcgtcgtt acgctctcgc cggcgcacca ccgagagaac gagtgcgacg atcaagaacg
     2041 tgaagacgcc caccacggcg agcacctcga gcagacgaac gaagaagtga tcgatgagac
     2101 ctcgtcctac ggtatcaagc ttggccgtcg ccgcgatcaa ctcttcatcg agcatgctca
     2161 tcgccgagat ccgctcttgc ccgatggcct cgagtatcgc ggcacgctcc acagcgactg
     2221 cttcgaaaat ctcgcggcgc tccctcgcga tggcgtcgag aagcgtcgag acctgtccat
     2281 ccatggtcga ttcgaagtcg tcgagaaaac cagtgaattt ctccgctgtg gcaaccaccg
     2341 catccgcggg ctcttgaatc cgctcctcga agagcgcctg aacgaggtag tcggcctgcc
     2401 aacgcgcttc cacaggcaac tggaccgtga ggatggccag ccgatcgttg aggtcgcgga
     2461 acgtctcttc catgctaccc accgcttgga ggccgccctg ctttgcttcc gagacgagcc
     2521 ccgcaaggtc agctcgcgca gttgggcgca ccatcagctg accttcgatc ggatggtccg
     2581 cgatccaggc gcgcacgtcg tccggcagtt ccctccgctc tttgtcgttc atgacggtgt
     2641 aagccaactg ctcagcttgt tcttcgagct gcttggtcgt ttcagacacg cacccgggta
     2701 gctcgccaaa gtagcgcgtg ccctcgccct cggtgaagta ctgacgctgt tgaccggcca
     2761 ggacccaaag ctccagcaag ccggcgaccg gatcctgatc gaaggccgcc gcgcgcgcct
     2821 gcggcatggc ccacattcgc cactcatagg cgcgagcctt gatgtatggg tcctccgtct
     2881 gggcgacgat ctgcatgacg cacattgcga ccgattcacc gaatcgcgtc gcatactggt
     2941 agtccatcgc gcgcaactga gcgacggtga catcgtccct ggccatattg accatcagag
     3001 gtggtctagg cggcgcgctg ctgcagccaa aggcgaacgc gatcaaagcg ctgatgacga
     3061 ggacattcga tctatgattc gcgattggca acctaggtac ttcgcgcggc ttccggcatg
     3121 cccggccaaa gtattcggct gggtctgtgt cagtcaaccc tcgggatcgg gccgtcagcg
     3181 cgacctccta gccagcctgg agggccaaat ttgaaggcat acggctagcc ccggagcatc
     3241 tctcgccgat caaagcccaa cggcgctgac tctgcgccgt tctcgcggta tcctcccggc
     3301 ccccagcaac tctactcgag catcagtaac gaggtacatc cagatgactg aagagaatta
     3361 cggcctcggc acacgtgccc ttcatgccgg acaagaaccc gaccccacga ccaattcgcg
     3421 cgccgtgccg atttacgcga ccacctctta cgtcttcaac gacaccgatc atgcggcagc
     3481 gctcttcggg ctggccgagt tcgggaacat ctactcccgt ttgatgaacc ccacggtcga
     3541 cgtcctcgaa aagcgcttgg cagcgctcga tggcggcgtg acggggctct gcttcgcgtc
     3601 cggtcaatcc gcgatcaatg cggcgattct cacgctctgc cattcgggcc agaatttcgt
     3661 ttcggcgacc agcctctacg gcggcacctg gaccctcttc aacaacacct tcaagaacct
     3721 gggcgtcgag gtccgtttct tcgacccggc gcatcccgag cagatccacg agctggtcga
     3781 cgagaacaca cggctcgtct acatcgagtc gatcggcaac cccaagaacg acgtgcccga
     3841 cttcaaggcg atcgccgagg ccgcgcacag cgccccgcac ggcgcgcttc ccctgatctg
     3901 cgacaacacg gtgatgacgc cgtacctgtt ccgccccttc gagcacggaa tcgacatcaa
     3961 cgtctactcc accacgaagt tcatcggcgg ccacggcacg cacatcggcg gcgccatcgt
     4021 cgattcgggc aagttccaat ggaccgagaa ccgcgagaag tggcccgagt tctgtgggcc
     4081 ctcgcccaac tatcacggcg cggttctggc cgacgccctc gctccgatgg ggaacatcgt
     4141 ctacaacgtt caaattcgta cccactggct tcgtgacacg ggcgcggcga tgagcccgtt
     4201 cgcagccttc ctcttccttc aagggctcga gaccttgcac cttcgcatgc ctcggcattg
     4261 cgagaacgcg caggcggtcg ccgagcatct cgagaaacac cccgccgtac agtgggtgaa
     4321 ctacccaggc ctcccgtcgc acccggacaa cgagcgggcc aaccagtatc tgccggacgg
     4381 caagggcgcc atcctcggtt tcggcatcaa gggcggcatg gaagcgggca agaagttcat
     4441 caactcggtc aagctctgct cgcatctcgc gaacatcggc gacgccaaga ccttggtgat
     4501 tcaccccgcc tcgacgaccc accagcagtt gagtccagcc gagcaacagc aagccggtgt
     4561 cgtgcccgag tacgttcgcg tttcggtcgg tatcgaagac gtgaaagaca tcatcgcaga
//
LOCUS       DASRZF010000028        16785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4147165, whole genome shotgun sequence.
ACCESSION   DASRZF010000028 DASRZF010000000
VERSION     DASRZF010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 16785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16785
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4147165"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VFG22_01270"
     CDS             complement(<1..258)
                     /locus_tag="VFG22_01270"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414895.1"
                     /translation="MVDPTETAAWKALRLHYDEVADLQMRQLFQDDPDRASRLSLRLG
                     ELLFDFSKNRITEQTLGFLIELCEECGVPDWIARMFSGEPIN"
     gene            complement(363..2447)
                     /locus_tag="VFG22_01275"
     CDS             complement(363..2447)
                     /locus_tag="VFG22_01275"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HET6414896.1"
                     /translation="MENLIYAAPVAGVLALIFAWTKASWVSKQDPGDANMVEIAGQIQ
                     EGALAFLRAEYRVLSIFVLIVAAILGVAYYGDPERGWQIAGAFVVGAASSAAAGWIGM
                     KVATNANVRTTAAARIGLSPALDVAFSGGTVMGMTVVGLATLGLGVLYFVFTTVLGWD
                     LTRTVNVITGFSMGASSIALFARVGGGIYTKAADVGADLVGKVESGLPEDDPRNPAVI
                     ADNVGDNVGDVAGMGADLFESFVGAIIGAMVLALGFDAVSDGTSVGPVILPLVIAGAG
                     IICSMVGTYFVRTKEGGNPQHALDMGAFGAAGVMAAATFAIAKYMWPEGGATTLQGEF
                     VTWANVGWATVAGLAAGVAVGLITSYYTSMDKGPVNSIAEQSKTGTATNIIAGLGVGM
                     QSTALPILVIAAGVVVAADVAGLYGVAIAALGMLSTTGIQLAVDAYGPIADNAGGIAE
                     MSHQAPQVRERTDKLDAVGNTTAAIGKGFAIGSAAMTALALFAAFAQTAGISGINISK
                     PLVMAGLFLGGMLPFLFSSMAMKAVGIAAMDMIEEVRRQFREIPGLLEGTAKPDTARC
                     VKISTEASLKRMLAPGALAILTPVVVGFAMGPEALGGLLAGVTVSGVTMALFMANAGG
                     AWDNAKKSFEGGGYKMSDGTVHPKGSEAHNAAVVGDTVGDPFKDTAGPSLNILVKLMS
                     IVALVLAPLIAI"
     gene            complement(2576..3505)
                     /gene="fabD"
                     /locus_tag="VFG22_01280"
     CDS             complement(2576..3505)
                     /gene="fabD"
                     /locus_tag="VFG22_01280"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HET6414897.1"
                     /translation="MGKVAFVFPGQGSQKVGMGREAFESSDLARAVFDEADEALGEPI
                     SKLCFEGPEESLQLTANTQPAVLTASIAILRALDAPFDVVAGHSLGEYSAHVAAGTLR
                     LADAVRLVRKRGQYMQEAVPYGEGAMAAVLKADRTLVERICAEVPGLVEAVNYNSPQQ
                     IVIAGETAAVSEAGIKLKEAGARSSTLPVSAPFHSTLMEPAEERLREDIGAIAFSKPR
                     VPVYVNVDAVPLSDLGALKEALIRQVSRPVLWEESVRRMIDDGVSLFVEVGPGRVLSG
                     LLARIDKRAGRVSVQGPSDFDPAREAIEEARAS"
     gene            3543..4007
                     /locus_tag="VFG22_01285"
     CDS             3543..4007
                     /locus_tag="VFG22_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414898.1"
                     /translation="MEEEPNAEDREHLSALSIGHFILAGVALLGGVPTLLIGVSGSKL
                     MDEFGSDLSMAMGQIPGQSGVDPFGGSPDAMLQDLNTLIISVVVSMVLLAVVSAIHLA
                     VVGVQIRNRRWWTFCYLTGWGECLMFPFGTVLGIFTIIVLSRPSVKRLFGLR"
     gene            4073..4327
                     /locus_tag="VFG22_01290"
     CDS             4073..4327
                     /locus_tag="VFG22_01290"
                     /inference="COORDINATES: protein motif:HMM:NF013851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HET6414899.1"
                     /translation="MLEAEVLEERIRQGVGEVSHIVVRDLTGTKDHWEALVVSAAFSG
                     KTPIEQHQMVYAALGELMTGPVHALALKTYSPESWAKHEG"
     gene            4331..4651
                     /gene="grxD"
                     /locus_tag="VFG22_01295"
     CDS             4331..4651
                     /gene="grxD"
                     /locus_tag="VFG22_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HET6414900.1"
                     /translation="MDDSLRERIQGIIDANDVVLFMKGTKHFPQCGFSATLVEVLRRS
                     GSEFQDVNVLEDPGIRQGIKEFTNWPTIPQLYVHGKFVGGCDIVREMYEKGELDAVLS
                     AKES"
     gene            4669..5715
                     /locus_tag="VFG22_01300"
     CDS             4669..5715
                     /locus_tag="VFG22_01300"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6414901.1"
                     /translation="MAELLGGKYEVLELAGEGGMAKVYRGQTHGAAGFTRPVAIKRVL
                     APLSQNPEFLKMFVEEARVVSELDHPNIAQIHDFDRDRTGEYYLVLEWVDGLTLAEWR
                     QGYRAHGNHTPWSLTAAIGIEVLEALHAAHVHEDAEGRPAPIFHRDVTPQNVMLSKCG
                     VVKLTDFGLARAMDRSSITKPGIVKGKISYLAPELTYEAEPSAQTDVFSVGVVLWEVL
                     AGEKLFKGKDPIKVLGTIRGTETPDLIAFRDDLPKRLREIIAKALQRNPIERYGSTRE
                     MARDLADLLRASEEITDSHVIALSVHWAAKQLEHAEPEEPPTQPFAARDALLQTGSAE
                     ETVPDTSIPLTRRK"
     gene            complement(5734..6294)
                     /locus_tag="VFG22_01305"
     CDS             complement(5734..6294)
                     /locus_tag="VFG22_01305"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HET6414902.1"
                     /translation="MNNTWVRLSLLAAFLLGVYAIGWATGFTEGLTVDGIRDTMRTAG
                     VAGFAVFVAIFCVGQLLYIPGFVFVMVSGLAYGPVWGSIASMIAATVSVAVSFIIVRT
                     IGGQPLKDLNIKRPFLQKPLDHLEERPIRSMIVIRLFLWAIPPVNYTLAMSGVTFRDY
                     MIAAVIGMTPPFVALSVAAGLGFNLL"
     gene            complement(6381..8327)
                     /locus_tag="VFG22_01310"
     CDS             complement(6381..8327)
                     /locus_tag="VFG22_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414903.1"
                     /translation="MFGRRSTLVAVLVIGALLSSARVVAEDMMGPLQLEGEEADAEEL
                     ALWELIEREHYVKAREDAETYLAEHPRSYVAHLVLGMAQHYGEANFPKALFHETRALD
                     LFEERQGTQPTPSDPWRWHARILMELTHAHGDLEHYDQQLGFMQRYNELYTPQLKAEL
                     AWPLMKKREFEKARLAAEEGLATGDPYQIERAKNALCAIEFEAGDDEKSYEVCADAVA
                     YGRTHFGNATAVDLGNYAEAARSVFRFDEAERLLMEASKSGLSWYGNPWLELADLYMR
                     AGRFAEALSALREVPRHRSVRPAHVRDSDRNEARRSLSAFLVLMGRPAEALRITDKAS
                     VMPDRRAHNSRDPEQDQSIVALLDRQARLMMAEMTLERTSARPFYVRWWAEVQALWIR
                     FQAWMSARQAARFLDDEARLVGTFAIGTARSAVMPPWLLGELIGALGPGVVRAAVDAV
                     SLEDPREGAVPYHESVLAEVALAQREYAEAIAHAERALERLQPGDALLRERVRAVLAK
                     ALSDPREATALYEEVLSTDPGLFRRLGWALPVEVDSSDAEVDRALAKALMRSPRFEQS
                     SDGLRIQIEGAKVCLFGRSGTAWGCSEVEVGEDETQESYTQRIVDSFHAEIFSPRVDL
                     SRIDINSLDGSNRVTRNPLDMIVP"
     gene            complement(8330..9343)
                     /locus_tag="VFG22_01315"
     CDS             complement(8330..9343)
                     /locus_tag="VFG22_01315"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6414904.1"
                     /translation="MQQAIQSPIDDTLSGTREVAERLGARLKAAIRGRDDVVELVLIA
                     LLADGHVLLEDYPGSGKTTLARALGNALAEKDASSSIAAFRRIQFTPDLLPSDITGTN
                     IFDVETSRFAFRRGPVFAHIVLADEINRTSPKVQAAMLEAMAEKQVTVDNQSYLLDDV
                     FFVIATQNPLDLAGTYPLPTPQLDRFLFKIEMKHIDRASELEVLDQYPRASLDVARDY
                     PSVTLEEILAARRQTRSAVHVAPVVKEALVDLARLLRSDPRVLQGASTRALVLMLPAL
                     QARAVIHGRDFVSPEDIQVLAPYVFKHRLECAPGVEDVDALIAEATAVEIEKLARASL
                     RRG"
     gene            complement(9377..11548)
                     /locus_tag="VFG22_01320"
     CDS             complement(9377..11548)
                     /locus_tag="VFG22_01320"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HET6414905.1"
                     /translation="MSAQEDSMGLSRTLAEVGRAAIHGLAWGVLVATMAPSGSALVAF
                     AGGFGGCLLGAQLAHTRLRTSAIVAGALALLSLVFWLGASLTAGSFLSIALGPVGALR
                     VSGMLTALLVAAISSAAMRAGSSRKALFAVFELLVVAVAFAQLFIPHRYGAINRPFYL
                     ADWIIAFGWDPTWLFLFMGGTATVFGLVLLLREQRFGRAVLNLGVIGLLLALIVLATP
                     MLGMPPPPDGGGGLGLRPDPSEANAGEGERRERGGSTSSELEFRNKYNTGGARVPVAV
                     VLFHDDYSPPSGVYYFRQGAFSQYNGRKLVVSGRQDIDDDLIPYFPTRLTEIRNAPAS
                     NENRGPLETTIALMADHTQPFALEAPIEVEPLENPSPNRFRRVYRALSGVLTADYASM
                     LDARLGSPDWSKEQWAHYTEAPPDPRYGALAAEVAGELPDELASNPIARAFAVAAWLG
                     REGIYSLKNEHSGAEDPTASFLFGDRTGYCVHFAHAATYLMRSLGVPARVATGYAVSE
                     SSRQGGSSLLLSGAASHAWPEIYIDGYGWVVLDVYPERALDPPEQPMDADLQRLLGEL
                     ARGASPIPLSATDLPKIKELARRVTLTAGAWLFALLALALLILYLVKLWRRLAPAWAP
                     AASLPRVVYRAELDRLGELAIRRTPGESRESFARRLGESLPSLGPLTDTHVAAAFGQR
                     RPEPHQLRDEAANLRRDIRRRFPLWRRFLGLLTPWSWLRSK"
     gene            complement(11545..12867)
                     /locus_tag="VFG22_01325"
     CDS             complement(11545..12867)
                     /locus_tag="VFG22_01325"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET6414906.1"
                     /translation="MRPLIVVPGPIQGVWTRGRGLARRAADLWPLTALGMALGLVSSI
                     ALVSFGFKKLDLVLLVLGYGGAGLLLLSTLLVVLSSSALWLRLRRASIPWLTSTFETG
                     AALRTGFSLPSLWWLPMVQLRWTWASPEASVEEPLSERGRLRERVTLHKRGVFESVER
                     RVVVQDAFGLARIAFRHRQSGPIEVLPHLGGIRRLPVLTSLTGGEEFPHPMGLEDGDR
                     VELRRYVPGDSARFIHWKVFGRTRTLMVRVPERALSRARRTVAYLVAGPHDEAASAAA
                     RAAIEEEVLGADWQFAADGSPQATSNRTEAMLKVMRSSAFVGRSGAGMKQFLAEVDPA
                     GPAALVVFAPPTMGEWLDEMRSLSARRYGRIRVVIAVDAVHDPSTQPGWRRWLLMPTA
                     SPGVERADLERVGRALGQMRCEVVIIDRVSGRVLGDGQHLDRARRRAA"
     gene            13072..14040
                     /locus_tag="VFG22_01330"
     CDS             13072..14040
                     /locus_tag="VFG22_01330"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6414907.1"
                     /translation="MSDVMIDARDLTKQYGPVTAVKNATFQVNRGEVVGFLGPNGAGK
                     TTTMKILTCFIAPSGGTARVAGADIFEDPISVREAIGYLPESTPLYTEMLVLEYLEFV
                     GKMRELRGAGLQARLRRVVEETSLGDVIGKSIGELSKGYRQRVGLAQALIHEPPVLIL
                     DEPMSGLDPNQASEIRQLIREIGRERTVILSTHNLAEVQVTCGRVLIIANGQLVADDT
                     PEELAARAGKPRFVATIRKDGLSESAIRDAFSEIIDAQSVKQKTDAAAGEVVVEIVPK
                     GNQDLRANIFHAAVSANIVLLGLEQRGENLEDIFRQLTTGNSGVPS"
     gene            14037..14747
                     /locus_tag="VFG22_01335"
     CDS             14037..14747
                     /locus_tag="VFG22_01335"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6414908.1"
                     /translation="MRQVLTIGGRQFRSYFNGPVAYIVICIVMLTLGFFFWKTFFLFS
                     RASAREMFRWLGLIMVFALPALTMGLLAEEKRTGTIELLITMPVTEAQVILGKFIGVL
                     GLYAVILALTVFYPISVSTLGDLDWGPVLSGYLGLLLQGSAVLAIGLMASSWTSNQLI
                     ALFVALTLSVFFWVLDKFLAILPTNAASTLEWLSFDHHFQSMARGVIDLRDVLFFVSV
                     AVFALALAFRALESRRWS"
     gene            14751..16460
                     /locus_tag="VFG22_01340"
     CDS             14751..16460
                     /locus_tag="VFG22_01340"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HET6414909.1"
                     /translation="MSSKTQKRAASESMLFLSVVGGILILLNVLAAYLPSPRVDLTRN
                     GLFSLADGSERVAANLDDRLEVTAYFTENLPPPFNATERQVRDLLSEYAAASNGKIRV
                     RFINPDDEAKQQAARADGVQPVAHQKIEEDQVAVVEGYRGMVLSYLDQTRTIPVIQDT
                     AGLEYIITSAIKELVGERKPIGIVSGHGGPSLEQGLTALQSVLQLYDIQEIDATQEID
                     PSLAALLIIGPQAPFSDDELRRIDQYVIRGGSLGVFGGALAIDLAGESGPSARPVDAR
                     LDGLLEPWGVKLSSKIVADAQCSRAPMRGPMGLQVLVPYPPIPVLQLEQEQLDHPVMF
                     RLASPMLPFVAPIELGEAPDGVTLTVLASSSQDSWTMSGPTISLEPRNPRDWSMSSDA
                     GPFTLMVAIEGKLPSAYSAPIDEAEPSAIQTPPLAENDVRVLVTGTSTFLEDSFMPPR
                     TQQNDVQMNAALALALNAIDWLAADSDLIAIRAKTTEEPALDIPDSVLAAENTALAAA
                     EEGDEAGVESALSIRKAAIESWDAKKRAYRWLNTLGIPFLVVLFGLFRWRRRVDKKKA
                     LQL"
     gene            16479..>16785
                     /locus_tag="VFG22_01345"
     CDS             16479..>16785
                     /locus_tag="VFG22_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414910.1"
                     /translation="MDWRKNRIVIGAVAFCLLLGLTFWVIGGRNQQPGETEELPSLDL
                     DKEAITSLEVIRPGQEPVVLSKSDGAWRVLKPLDAAADQTNVESALNRLADLRIKRVV
                     "
BASE COUNT         3273 a         5308 c         5274 g         2930 t
ORIGIN      
        1 gttgatcggt tcgccggaga acatccgcgc gatccaatcc gggacgccgc actcctcgca
       61 cagctcgatt aggaagccga gggtctgctc ggtgatgcga ttcttggaaa agtcgaatag
      121 cagctcgccg agtctgagcg ataatcgaga cgcgcgatcc gggtcatcct gaaagagctg
      181 acgcatctgg aggtccgcca cctcgtcgta gtgcagtcgc aacgccttcc atgcggcggt
      241 ctcagtcggg tcgaccatcg ccgtagccta gctccaaaga aaaaccccgt acaagcgacg
      301 ctctgcatcg ctgacggggc ttggcccccg aaggggcggc aatactccaa cggaatcgtc
      361 gactagatgg cgatgagcgg cgcgagaacc agagctacga tgctcatgag cttcacgagg
      421 atgttgagcg agggacccgc ggtgtccttg aaagggtcac cgacggtgtc gcctaccacg
      481 gcggcattgt gagcctcgct gcccttcggg tgcactgtgc catcgctcat cttgtaaccg
      541 ccaccttcga acgacttctt ggcgttgtcc caagcgccac cggcattggc catgaatagc
      601 gccatggtga cgcccgaaac ggtgacaccc gcgagaaggc ctcccaaggc ctcaggaccc
      661 atggcgaagc ccacgaccac gggggtgaga atggcgaggg cccccggtgc aagcatgcgc
      721 ttgagcgagg cctcggtgga gattttcacg cagcgcgccg tgtccggctt tgccgtaccc
      781 tcgagcagcc ccggaatctc tcgaaactgg cggcggacct cttcgatcat gtccatggct
      841 gcaatgccga ccgctttcat cgccatcgac gagaagagga agggaagcat gccgccaagg
      901 aacaggccgg ccatgacgag gggcttcgag atgttgatgc cgctgatgcc tgccgtctgt
      961 gcgaaggccg caaagagcgc gagggctgtc atggccgccg agccgatggc aaatcccttg
     1021 ccgatcgcag cggtcgtgtt gcccacggca tcgagcttgt cggtgcgctc acggacctga
     1081 ggcgcctgat ggctcatctc ggcgatgcct ccggcgttgt ccgcaatggg gccatacgcg
     1141 tcgaccgcga gctggatgcc cgtggtggag agcatgccga gcgcagcgat ggcgacgccg
     1201 tagaggcccg ccacatcggc ggcgacgacc acacctgccg caatgacgag aatcggcagg
     1261 gcggtcgact gcatgccgac accgagcccg gcgatgatgt tggtggcagt gccggtcttg
     1321 ctctgctctg caatgctatt gaccgggccc ttatccatcg acgtgtagta cgaggtgatg
     1381 agtccgaccg cgacgcctgc agccaagccc gcgaccgtcg cccagcccac gtttgcccag
     1441 gtcacgaact cgccctgcaa ggtggtcgcg ccgccttcgg gccacatgta tttggcaatg
     1501 gcgaacgtcg cagcagccat cacgccagct gcgccgaaag cacccatgtc gagcgcatgc
     1561 tggggattgc ccccctcctt ggtgcgcacg aagtaggtgc ccaccatcga gcagatgatt
     1621 cccgcgcctg cgatcacgag cggaagaatc accggaccta cgctggtgcc gtccgacacg
     1681 gcatcgaagc cgagcgcgag gaccatcgca ccgatgatcg caccgacgaa cgattcgaaa
     1741 aggtcagcgc ccatgccggc gacgtcaccc acgttgtcgc ccacgttgtc ggcgatgacc
     1801 gcggggttcc gggggtcatc ctcgggaaga ccggactcga ccttgccgac gaggtcggcg
     1861 ccgacgtccg cggccttggt gtagattccg ccgccaacgc gcgcgaagag cgcgatcgaa
     1921 gaggcgccca tcgagaagcc cgtgatgacg ttgaccgttc gcgtgaggtc ccaacccaag
     1981 acggtggtga acacgaaata gagcacaccg aggccgagtg tcgcgagccc aacgaccgtc
     2041 atgcccatca ccgtgccccc ggagaacgcc acgtcgagcg caggggacag gccgatgcgt
     2101 gccgccgccg tggtgcgcac attggcgttc gtcgcaacct tcatgccgat ccaaccggcg
     2161 gcagccgaag aggccgcacc gaccacgaag gcaccggcga tttgccagcc tcgttccggg
     2221 tcgccgtagt acgccacgcc cagaatcgcc gccacgatga gcacgaagat cgacaggacg
     2281 cgatactcgg cccgaaggaa cgcgagggcg ccctcctgaa tctggccggc gatctcgacc
     2341 atgttggcgt cgccaggatc ttgcttagaa acccaactcg ccttcgtcca ggcgaagatc
     2401 agagcgagca cgcccgctac tggggctgcg taaatcagat tttccatagt cgctgaacct
     2461 ctattcgggc ccgtccccgg ttaactgctg gcgggcggcg cggttctaca agatttgaaa
     2521 atcaggtcaa acgcagggcg cgagtttcag acacacgcct gcaattacaa gggaattagc
     2581 tcgcccgggc ttcctcgatc gcctctcgag ccggatcgaa gtccgacgga ccctgcacgc
     2641 tgacccggcc tgcacgcttg tcgatccgcg ccaggagacc gctgaggacc cgccccggac
     2701 cgacttcgac gaagagagaa accccgtcgt cgatcattct tcgaacgctt tcctcccaaa
     2761 ggaccggtcg actgacttga cggatcagcg cctccttcag tgcgccgaga tcgctgaggg
     2821 gaaccgcgtc cacattgacg tacacgggca cacgaggttt ggagaacgcg atggccccga
     2881 tgtcctcgcg caagcgctcc tcggccggct ccatcaaggt cgagtgaaac ggggcgctga
     2941 ccggaagtgt gctggagcgc gcgccggcct ccttgagctt gattcctgct tcgctcaccg
     3001 ccgcggtctc gccggcgatg acgatctgtt gtggcgagtt gtagttgacc gcttcgacca
     3061 ggccagggac ctcggcgcag atgcgctcga ccagcgtgcg gtcggctttg aggaccgccg
     3121 ccatggcgcc ctcgccgtaa ggcacggcct cttgcatgta ctggccgcgc tttcgcacga
     3181 gtcgcacggc gtcagctagc cggagcgtgc ccgcagcgac atgtgcggag tactcaccga
     3241 gactgtgccc agcaacgacg tcgaacggcg catcgagcgc gcgaaggatg gcgatggatg
     3301 ccgtgaggac agcaggctgt gtgttggctg tgagctgaag agattcctca gggccttcaa
     3361 aacagagctt cgaaatcggc tccccgagag cctcgtcggc ttcgtcgaac accgcgcggg
     3421 ctagatccga ggactcgaat gcctctcggc ccatgccgac tttctgactg ccctgccctg
     3481 ggaaaacgaa tgcgaccttg cccatcgagt gcctgcatag cacgatccat ttgggggttc
     3541 gcttggaaga agagccaaac gccgaggacc gagagcattt gagcgcactg tcgatcggcc
     3601 atttcatcct tgccggcgtc gcgctgctcg gtggggttcc cactttgctg atcggcgtct
     3661 ccggatcgaa gctgatggac gagttcggaa gcgacttgtc gatggccatg ggccagatcc
     3721 ccgggcagtc gggtgtcgat cctttcggag ggagccccga cgcaatgctt caggacctca
     3781 acaccctgat catctccgtc gtcgtgagca tggtgctgct cgctgtcgtg agcgccattc
     3841 acctcgccgt cgtcggagtg caaatccgaa accgccgctg gtggacgttc tgctacctca
     3901 caggctgggg cgagtgcctg atgtttccat tcggcaccgt gctcggtatt ttcacgatca
     3961 tcgtcctatc gcgcccttcg gtgaagcgcc tgtttggcct gcgttgaccc tcccggcgat
     4021 tgacaattgc gtagcccggc cgtagccaag agcgttgagt ggaggatgct cgatgcttga
     4081 agcggaagtc ctagaagaac gaattcgtca gggtgtgggc gaggtgtccc atatcgtggt
     4141 cagagacctg accggcacca aggaccactg ggaggcgctc gtggtgtccg cggcattctc
     4201 tgggaaaaca ccgatcgagc agcaccagat ggtctatgcg gccctcggcg agctgatgac
     4261 cgggccggtt cacgcacttg cgctaaagac gtactcgccc gagagttggg caaagcacga
     4321 gggttgagag atggacgaca gcttgcgaga acggattcag ggcatcatcg acgcgaacga
     4381 cgtcgtgctc ttcatgaagg gcacgaagca tttcccacaa tgcgggttct ccgcgacctt
     4441 ggtcgaggtg ctgcggcgct caggctcgga gttccaggac gtgaacgtcc tcgaggaccc
     4501 gggtatccgc caagggatca aagagttcac caactggcca acgatcccgc agctttacgt
     4561 ccatggcaag ttcgtcggcg gctgcgacat cgtgcgagaa atgtacgaga agggtgaact
     4621 cgacgccgtg ctttctgcta aagagagttg aggcgtcagc cgcccgacat ggcagagctg
     4681 ctcggcggaa aatacgaagt tctcgagctc gccggtgaag gcgggatggc gaaggtctat
     4741 cgagggcaga cgcacggcgc cgcgggattc acgagaccgg tggccatcaa gcgcgttctc
     4801 gccccactca gccagaaccc cgagttcctc aagatgttcg tcgaagaggc gcgcgtcgtt
     4861 tcggagctgg accatcccaa catcgcgcag attcacgact tcgatcggga ccgtactggg
     4921 gagtactacc tcgttctcga atgggtcgac ggactcacgc tcgcggagtg gcgccagggc
     4981 taccgggcgc acggcaacca cacgccctgg agcctcaccg cagccatcgg catcgaggtc
     5041 ctcgaagcgc tccacgccgc ccacgttcat gaggacgcgg aagggaggcc cgcgcccatc
     5101 tttcatcgag acgtcactcc gcaaaacgtg atgctgagca aatgcggcgt cgtgaagctg
     5161 acggacttcg gtcttgcgcg cgcgatggat cgaagctcga tcacgaaacc aggcatcgtg
     5221 aagggtaaga tttcctatct cgcgccggag ctgacctacg aagcggagcc gagcgcacag
     5281 accgatgtat tcagtgtcgg cgtggttctc tgggaggttc tggcggggga aaagcttttc
     5341 aagggcaagg atccgatcaa ggtgctcggt acgattcgcg gaacggagac cccagacctc
     5401 attgccttcc gtgacgacct cccgaagcgc ttgagggaaa tcatcgcaaa agcgctccag
     5461 cgaaatccga tcgaacggta cggctcgacc cgcgagatgg ctcgcgatct cgcggatctg
     5521 ctgcgcgcga gcgaggaaat caccgactcc cacgtgatcg ccctgagtgt acactgggca
     5581 gcaaagcaac tcgaacacgc ggagccggaa gaaccaccga ctcaaccgtt cgcagcgcga
     5641 gatgcgcttt tgcaaaccgg ctctgccgaa gaaaccgtcc cggacacgag cattccctta
     5701 actcgccgga aatagatacc taggggcgcg gtcctagaga agattgaagc ccaaacctgc
     5761 cgcaaccgaa agagccacga acggcggagt catgccgatc accgcggcga tcatgtagtc
     5821 gcggaaggtc acgccggaca tcgcaagcgt gtagttcacg ggcggtatcg cccagaggaa
     5881 gaggcggatg acgatcatcg aacggatcgg cctctcctcc agatgatcga gaggcttctg
     5941 gagaaacggg cgtttgatgt tgaggtcttt gagcggctga ccgccgatgg tgcgaacgat
     6001 gatgaatgac acggcgacgg acaccgtggc ggcaatcatc gatgcgatcg aaccccagac
     6061 gggaccgtac gcgagcccag acaccatcac gaagacgaag cccggaatgt agagcagctg
     6121 accgacgcag aagatggcga cgaacaccgc gaatccagca actccggcgg tccgcatcgt
     6181 gtcacgaatg ccgtcgaccg tgagtccttc tgtgaatccg gtcgcccagc cgatcgcgta
     6241 gacgccgagc agaaacgcgg ccagcaacga gaggcgaacc caggtattgt tcacgagcgc
     6301 cagtaagaca cagacccgcc ctcgagacca ggtgcccctt ggaagcggtc gcgctttggc
     6361 tttttgcagg tcagcaccgg ctaaggaacg atcatatcca gcggatttcg cgtgacgcga
     6421 ttcgacccgt ccagcgagtt gatgtcgatg cgactgagat cgactcgagg agagaagatc
     6481 tctgcgtgga aagaatcgac gatgcgctga gtgtagctct cttgcgtttc gtcttcaccg
     6541 acttcgacct cgctgcaacc ccaagccgtg ccgctgcggc cgaacaggca gaccttcgcc
     6601 ccctcgattt ggatccgtag tccgtcactg gactgttcga aacgcggtga acgcatgagt
     6661 gcctttgcaa gcgcgcgatc gacttccgca tcgctcgagt ccacctcgac cgggagcgcc
     6721 catcccagac gacgaaagag acctggatcg gtcgagagaa cttcctcgta gagcgcggtc
     6781 gcttctcgcg ggtcggaaag cgctttcgca agaacggcgc gaacgcgttc tcgcaggagc
     6841 gcatccccgg gttggagacg ctccagtgct cgctctgcgt gcgcgatcgc ctccgcgtac
     6901 tcacgctgcg ccaaagccac ctctgcgagc acagactcat ggtacggaac cgcgccctct
     6961 ctcgggtcct ccaggctcac tgcgtcgact gcggccctca ccaccccagg accgagcgcg
     7021 ccaatcagct cacccaagag ccaaggcggc atgacggcac tgcgagctgt gccgatggcg
     7081 aacgtcccga cgagcctagc ttcgtcgtcg agaaagcgag ccgcctggcg cgcactcatc
     7141 catgcctgaa aacggatcca aagggcctgc acctctgccc accagcgtac gtagaacggt
     7201 ctagcgctag tgcgttcgag agtcatctcc gccatcatca agcgggcttg cctgtcgagt
     7261 agagcaacga tgctctggtc ctgctcgggg tcgcgactgt tgtgagcgcg ccggtctggc
     7321 atgacagagg ccttgtcggt gatgcgtaac gcctcggcag gcctccccat caacacgaga
     7381 aacgcggaga gggagcggcg tgcctcgttg cgatccgagt ctcggacgtg cgcgggccga
     7441 acggatcgat ggcgcggcac ctcgcgaagc gcggacaggg cttcagcgaa gcggcccgct
     7501 ctcatgtaga ggtcggcgag ctcgagccac gggtttccgt accacgatag cccgctcttc
     7561 gaggcttcca tcaagagacg ctctgcctcg tcgaatcgaa acacagagcg cgccgcttct
     7621 gcatagttcc cgaggtcgac cgccgtcgca ttgccgaaat gcgttcgccc gtatgccacg
     7681 gcgtcggcac agacctcgta gctcttttcg tcgtccccgg cttcgaattc gatggcgcac
     7741 agcgcgttct tggcccgttc gatctgatac gggtccccgg ttgcgagacc ctcttccgct
     7801 gccaaacgcg ccttttcgaa ttcacgcttc ttcatcaagg gccacgccag ctcagccttg
     7861 agttggggtg tgtagagctc gttgtaccgc tgcatgaacc cgagctgctg gtcgtagtgc
     7921 tcgaggtcgc cgtgcgcgtg ggtgagctcc atcagaatac gcgcgtgcca tcgccaaggg
     7981 tcgctcggtg tcggctgcgt cccttgacgt tcttcaaaga ggtcgagcgc tcgagtttcg
     8041 tgaaaaagcg ccttcgggaa gttggcctcc ccgtagtgct gcgccatccc caagaccagg
     8101 tgggctacgt aggaacgcgg gtgctctgcc aagtatgtct cggcgtcttc ccgcgccttg
     8161 acgtagtgct cgcgctcgat cagctcccaa agagccagct cctctgcatc ggcttcctcg
     8221 ccttcgagct gaagcggccc catcatgtcc tcggccacga ccctcgcgga cgaaagcaaa
     8281 gccccgatga cgaggacggc aaccagagtt gacctcctgc cgaacatcat caaccgcgac
     8341 ggagactcgc gcgggcaagc ttttcaatct ctaccgccgt ggcctccgca atgagagcgt
     8401 cgacgtcttc cacgccgggc gcgcactcca aacggtgctt gaaaacgtat ggcgccaaga
     8461 cctggatgtc ttcgggggac acgaaatcgc ggccgtggat caccgcgcgg gcctgaaggg
     8521 ccggaagcat caatacgagc gcgcgagtgg acgcgccctg caggacacgc gggtcgctcc
     8581 tgagaagccg cgccaagtcc acgagggctt ctttgacgac gggggccacg tgcacggcgc
     8641 tccgagtctg gcgtcgagct gcgagaatct cttctagggt gacgctggga tagtcgcgcg
     8701 ccacgtccag gcttgcccgc ggatactgat cgaggacttc gagctccgag gcgcgatcga
     8761 tgtgcttcat ctcgatcttg aagaggaagc gatcgagctg aggtgtgggg agcggatagg
     8821 ttcctgcgag gtcgagcgga ttttgcgttg caatgacgaa gaacacgtcg tcgagcaagt
     8881 atgactgatt gtctaccgtg acctgttttt ccgccatcgc ctccagcatg gctgcctgga
     8941 ccttgggtga ggtgcggttg atctcgtcgg ccaatacgat gtgtgcgaac acggggccgc
     9001 gacgaaacgc aaagcggctg gtttcgacgt cgaagatgtt ggtgcccgtg atgtcactcg
     9061 gcagcaggtc aggtgtgaac tgaatgcgac gaaaggcagc gatgctgctc gaggcgtctt
     9121 tctcggccag cgcgttaccg agcgcgcgcg cgagagtggt cttgcccgag cccgggtagt
     9181 cttcgagaag cacgtgccca tccgcaagca gcgcgatgag cacgagctcg accacgtcgt
     9241 cccgtcctcg aatcgcggcc ttcagacgag caccaagccg ctccgcgact tcgcgcgtgc
     9301 cactcagggt gtcgtcgatg ggggactgga tggcttgctg catgtcagct tcgggctccg
     9361 tgggtgaagg atggcatcat ttcgagcgta accaagacca gggcgtgagc agccccaaga
     9421 aacgcctcca gagcggaaat ctgcggcgta tgtcgcgccg aagattggcc gcttcgtctc
     9481 gaagctggtg cggttcgggc cgccgctggc cgaatgctgc cgccacgtgg gtgtccgtca
     9541 gcgggccgag cgagggaagc gactctccga gacgtctcgc gaaggactcg cgggattcac
     9601 ccggcgtgcg gcggatcgcg agctcgccca ggcggtcgag ctcggcgcgg tagaccaccc
     9661 tcggcaagga ggcagccggc gcccaggccg gcgcgaggcg gcgccagagc ttcaccaggt
     9721 agaggatgag aagcgccagc gctaggagcg cgaaaagcca tgcacccgcg gtcagcgtca
     9781 cgcgccgggc gagctctttg atcttgggta aatcggtggc cgacagtgga atgggagaag
     9841 cgccgcgtgc gagctctccg agcagccgct gcaggtccgc gtccatcggc tgctcgggtg
     9901 ggtcgagggc acgctcggga tacacgtcga gcacgaccca gccgtatcca tcgatgtaga
     9961 tttcgggcca ggcatgtgac gcggcgcccg acaagagaag ggacgagcct ccctgcctcg
    10021 acgattcgct caccgcgtaa ccggtcgcca cgcgcgccgg aacgccgagg cttcgcatca
    10081 agtaggtggc ggcatgggcg aagtgcacgc agtacccggt gcgatcaccg aacaggaagc
    10141 ttgccgtagg atcttcggcg ccactgtgct cgttcttgag gctgtagatt ccttcacggc
    10201 cgagccaagc agccacggca aacgcgcgcg cgatcggatt gctcgctagc tcgtcaggaa
    10261 gctctcctgc gacctcggcc gcgagggcgc cgtagcgtgg gtccggcggc gcttcggtgt
    10321 agtgcgccca ctgctccttg gaccagtccg gggaacccaa tcgagcgtcc agcatcgatg
    10381 catagtccgc tgtgagaaca ccggacagcg cgcggtagac gcgacggaag cggttcggcg
    10441 atgggttctc gagcggctct acttcgatcg gcgcttcgag cgcgaagggc tgagtatggt
    10501 ccgccatcag ggcgatggtg gtctcgagtg ggccccgatt ctcgttgctg gccggagcgt
    10561 tgcggatctc cgtgaggcgg gtcggaaagt atgggatcaa atcgtcgtcg atgtcctgcc
    10621 ggccggaaac cacgagcttc cgtccgttgt actggctgaa agcgccttgg cgaaagtagt
    10681 agactccgct cggcggggag tagtcgtcgt gaaagaggac gaccgcaacg ggaacccgcg
    10741 cgccgccggt gttgtacttg ttgcggaact caagctcgct gcttgtcgat cctccccgct
    10801 cgcgtcgctc accctcgccc gcgttggcct ccgaaggatc tggccgcaga ccgagtccgc
    10861 cgcctccatc tggcggaggc ggcataccga gcattggcgt agccaagacg atcaaggcga
    10921 gcaggagccc gatgacgccc agattgagaa cggctcgacc gaagcgctgt tcgcgcaata
    10981 ggagaaccag gccgaagacc gtcgccgtgc cacccatgaa gaggaacagc caagtcgggt
    11041 cccagccgaa cgcgatgatc cagtctgcca gatagaacgg gcggttgatc gcgccatagc
    11101 gatgcggaat gaagagctgc gcgaacgcga cggcgactac gagaagctcg aagaccgcga
    11161 agagcgcctt gcgcgaagag cccgcccgca tggccgcgct gctgatcgca gcgaccagaa
    11221 gcgcagtcag catgccagag acccgcagcg cgccgaccgg gcccagcgcg atcgacagga
    11281 aagaaccggc cgtgagggac gcacccagcc agaagaccaa cgagagaaga gccagcgctc
    11341 cggctacgat cgccgaagtg cgaaggcgtg tgtgcgcgag ctgcgcgccg aggaggcagc
    11401 cgccgaagcc gccggcgaat gcgacgaggg ctgagcccga aggagccatg gtggcgacca
    11461 ggacgcccca ggcgagtccg tgaatcgctg cccgaccgac ctcggcgagc gtgcgggaaa
    11521 gtcccatcga atcttcttgg gcgctcacgc tgctcttctc cgcgctcggt cgagatgctg
    11581 tccgtcgccg agcacccttc cgctcactcg atcgatgatt acgacttcgc aacgcatctg
    11641 accgagcgcc cgccccactc gttcgagatc cgcacgctcg actcccggtg aggccgtagg
    11701 catgaggagc cagcggcgcc agcccggctg ggtgctcgga tcgtgaaccg cgtcgaccgc
    11761 gatcaccacg cgtatccgac cataccgtcg cgcggacagc gaacgcatct cgtcgagcca
    11821 ttcgcccatc gtgggcggtg cgaagacgac gagtgcggcg ggcccggctg ggtccacttc
    11881 agccaggaat tgcttcattc cagccccgct tcgaccgacg aaagccgagg agcgcatcac
    11941 cttgagcatt gcctcggtgc ggttggacgt ggcctgggga gaaccatctg cggcgaactg
    12001 ccagtcggcg ccgagcactt cctcctcgat tgcggcccgc gcggccgccg aagcggcctc
    12061 gtcgtgtggg cccgcgacga gataggcgac cgtcctgcgg gcccggctga gcgcgcgctc
    12121 gggcacgcgc accatgagcg ttcgagtacg gccaaaaacc ttccaatgaa tgaaccgagc
    12181 cgaatcgccg ggcacgtacc tgcggagctc cacccgatct ccatcttcga gccccatggg
    12241 atgtgggaac tcctctccgc ccgtgagtga ggtgagaacg ggcagccgcc ggattccgcc
    12301 gagatgaggc agtacttcga tcggaccaga ttgccgatgc cgaaacgcaa ttcgcgcaag
    12361 accaaacgca tcttggacga cgacccgccg ctcaaccgac tcgaagaccc cccgtttgtg
    12421 aagtgtgact cgctcgcgca gccgcccgcg ttcggagaga ggctcctcca ccgatgcctc
    12481 cggcgacgcc caggtccaac gcaattgcac catgggaagc caccagagcg atggaagcga
    12541 gaaacccgtc cgcaaagccg cgccggtttc gaatgtcgag gtcagccagg ggatcgaggc
    12601 ccgacgcagg cgaagccaga gagccgagct gctgagcacg acgagcaggg tcgagagcag
    12661 cagcagaccg gcgccgccat agccgagaac cagaaggacg agatcgagct tcttgaagcc
    12721 aaagctcacc agcgcgatcg aggacacgag gccaagcgcc atcccgagcg cggtcagcgg
    12781 ccaaagatcg gcagcacgcc gcgccagccc tcggccgcgg gtccataccc cttggatggg
    12841 gcccggaact acgatcagcg gcctcatgtt tgcgatcgtg ccacgggagt ctccaagctc
    12901 aaacaaccga aaattgtggt cattcccggc tccctccgtc cgggacggaa ggcacggaat
    12961 aagctcgccc tgcccagggt tgagggcgcc atgggtgtct tgacaggcct agagccgggg
    13021 ctaccctccg ccagccccgt agaacgcaac ggatcactcg cgagggagat tatgagcgac
    13081 gtgatgatcg atgcgcggga tttgaccaaa cagtacggtc cagtcaccgc ggtaaagaac
    13141 gcaacttttc aagtcaatcg cggcgaggtc gtcggttttc tcggacccaa tggcgccggg
    13201 aagaccacca cgatgaagat cctcacctgc ttcatcgcgc catcgggtgg aacagcccgg
    13261 gtcgccggcg ccgatatctt cgaagatccg atttccgttc gcgaagcgat cggctacctg
    13321 cctgaaagca ctccccttta caccgagatg ctggtgctcg agtacctcga gttcgtcggg
    13381 aagatgcgag agctgcgcgg cgcaggcctc caggcccggc tccggcgggt ggtcgaagag
    13441 accagccttg gcgacgtcat tggcaagagc atcggcgagc tttcgaaggg ctaccggcag
    13501 cgtgtgggcc ttgcacaggc actcatccac gagccgcccg tgctgatcct cgacgagccg
    13561 atgagcggct tggatcccaa tcaggcctcg gaaatccgcc agctcatccg tgagatcggc
    13621 cgggagcgca cggtcattct ctcgacccac aaccttgcgg aggtgcaagt cacctgcggg
    13681 cgcgtcttga tcatcgcgaa cggccagctc gtggccgacg acacacccga agagcttgcc
    13741 gcgcgcgctg gcaagccgcg gttcgtcgcc acgatccgga aggacggtct cagtgaatcc
    13801 gcgatccgcg acgccttctc ggaaatcatc gacgcgcaaa gcgtaaagca gaaaacggac
    13861 gccgccgcgg gtgaagtcgt cgtcgagatc gttccgaaag gcaaccaaga tctccgcgcc
    13921 aacatttttc acgccgctgt gtcggccaac atcgtgctcc tcggcctcga gcagcgcgga
    13981 gaaaacctcg aagacatctt tcgacagctc accacgggca actcgggggt cccctcatga
    14041 gacaggttct caccatcggc ggccggcagt tccgcagcta cttcaatggg ccggtcgcat
    14101 acatcgtcat ctgcatagtg atgctgacgc tcggcttctt cttctggaag accttcttcc
    14161 tgttcagtcg cgccagcgcg cgggaaatgt ttcgctggct tggactgatc atggtgtttg
    14221 ccctccccgc gctgacgatg ggccttcttg ccgaagagaa gcgcacgggc acgatcgagc
    14281 ttctcatcac catgccagtc accgaagcgc aggtgattct cggaaagttc atcggagtgc
    14341 tcggcctcta cgcggtcatc ctggccctca ccgtcttcta tcccatcagc gtctccacac
    14401 tgggggactt ggactggggc ccggttttga gcggctatct cggcttgctt ctgcaaggct
    14461 ccgccgtcct cgccatcggc ttgatggcgt cgagttggac cagcaatcag ctgatcgctc
    14521 tcttcgtcgc gctgaccctc agcgtcttct tctgggtgct cgacaagttc ttggcgattc
    14581 tgcccacgaa tgcagcgtcc actctcgaat ggctctcatt cgatcatcat ttccaaagca
    14641 tggcccgcgg cgtcatcgat ctacgagatg ttctgttctt cgtctccgtg gcggtgtttg
    14701 cgctggcgtt ggcgtttcgg gcattggaat cgaggcggtg gagctgaacg atgtcgtcta
    14761 aaacccaaaa acgcgccgcc agtgagtcga tgctctttct ttcggtcgtg ggcggcattc
    14821 tcattcttct gaacgtcctc gcggcgtacc tgccttcacc ccgcgtggac ctcactcgca
    14881 acggactgtt ctcgctcgcg gacggatctg agcgggttgc agccaatctg gatgaccggc
    14941 tcgaggtgac cgcgtatttc accgagaatc tgcccccacc gttcaacgcc acggagcgcc
    15001 aggttcgcga ccttctgtcc gagtatgccg ccgcttcgaa cggcaagatc cgggtgcgtt
    15061 tcatcaatcc cgatgacgag gccaagcaac aagccgcgcg cgcggacggc gtgcagcccg
    15121 ttgcacacca gaagatcgag gaagaccagg ttgccgtagt cgagggctac cgcgggatgg
    15181 tcctgagcta cctggaccag acgcgcacca ttccggtgat tcaggacacc gcgggtctcg
    15241 agtacatcat cacctctgcc atcaaggaat tggttgggga acgcaaaccg atcggcatcg
    15301 tcagcggaca cggcggcccg agcctcgaac aagggcttac ggccctgcag tccgtgttgc
    15361 agctctacga catccaggaa atcgacgcga ctcaagagat cgatccgagc ctcgcggccc
    15421 tgttgatcat cggtcctcaa gcgcctttca gtgacgacga gctgcgccgt atcgaccagt
    15481 acgtcattcg cggaggctct ctcggcgtct ttggtggcgc tctggccatc gaccttgccg
    15541 gcgagtcggg cccatcggcg cgcccggtgg atgccaggct cgacggcctg ctcgaaccct
    15601 ggggcgtgaa gctgtcgtcg aagatcgtcg ccgacgcgca gtgcagccgc gctccgatgc
    15661 gcggcccaat gggtctgcag gtcctggtcc cctacccgcc gatcccggtc ctgcagctcg
    15721 aacaagagca actggaccat cccgtgatgt tccgcctggc gtcacccatg ttgccgttcg
    15781 tcgccccgat cgagctgggc gaagctccgg acggcgtcac actcacggtc ctcgccagct
    15841 cttcgcagga ctcctggacg atgtcggggc ccacgatctc tctcgagccc cgcaatccac
    15901 gagactggtc gatgagctcg gatgccggcc ctttcacctt gatggtcgcg atcgaaggaa
    15961 agctaccttc ggcatattcg gcgccgatcg acgaagcgga gcccagcgcg attcagacac
    16021 caccgctcgc cgaaaacgac gtccgagtgc tagtgacagg aacgagcacg tttctcgagg
    16081 acagcttcat gcctcctcgg acacagcaga acgacgtgca gatgaacgcg gcgcttgcgc
    16141 tcgccctcaa tgccatcgat tggctcgcgg cggattcgga tttgatcgcg attcgcgcca
    16201 agacgacgga agagcccgcg ctggacattc cagactccgt gctggcggcc gagaacacgg
    16261 cgctcgcggc cgcggaggaa ggcgacgaag caggcgtcga gtcagcgttg tctatccgca
    16321 aagccgcgat cgaatcctgg gacgccaaga agcgggccta ccgctggctc aacacgctcg
    16381 ggattccatt cctggtcgtg ttgttcggtc tctttcgatg gcgacggcgc gtagacaaga
    16441 agaaagctct ccaactctag cgaagggaac gagtcgcaat ggattggcga aagaaccgta
    16501 tcgtgatcgg cgcagtggcg ttttgcctgt tgctgggact gacgttttgg gtgattggtg
    16561 gccggaatca gcagcccgga gaaaccgaag agctgccgtc gctcgatctc gacaaggaag
    16621 cgatcacgtc actcgaggtg attcgacctg gacaggagcc cgtcgtcttg tcgaagtctg
    16681 atggagcgtg gcgtgtcttg aagccactcg acgcggcggc tgaccagacg aacgtcgaat
    16741 ccgcgctcaa ccgcctagct gatctccgca tcaagcgcgt ggtgg
//
LOCUS       DASRZF010000029        20213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2428918, whole genome shotgun sequence.
ACCESSION   DASRZF010000029 DASRZF010000000
VERSION     DASRZF010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 20213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20213
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2428918"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1232)
                     /locus_tag="VFG22_01350"
     CDS             complement(<1..1232)
                     /locus_tag="VFG22_01350"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET6414911.1"
                     /translation="MRIISFFVVPLLVLGACSSSDREAEDVTIDTFNVALAGAFIPYE
                     AERRQPLAEAIAGMDSDVVCLQEVWTQADKELIRDMAVGNFPYSALFEDDLDTPVDDP
                     TDQMGEIPQAPTTVPCPDIEVSDGVTIVDQMNEAVDCVAENCSTIPGSEDGRTTSASC
                     ASTNCVGAVAALLFGDAQQQRCYSCVVPQLPTSTFAEIRSSCPTVVNQDLAFDGQNGV
                     MILSRYPLSDQENWVMPGTWNRRTILRATVTLPNGASLDAYCNHLTPIFDDFTFPYTG
                     QYGNGETGAAGWEAEQYLQAQKLIAFVQDRSGDSPAVILGDFNTGRAYPDQGISEEGE
                     ATLALLEASFPAALAADYEPLCTFCEDNPVADSASSVWIDHILLNGLTSDAVQSTVRT
                     FDEDVVPVGGDELVPLSD"
     gene            complement(1372..1815)
                     /locus_tag="VFG22_01355"
     CDS             complement(1372..1815)
                     /locus_tag="VFG22_01355"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HET6414912.1"
                     /translation="MASRYRHLRARALKSMFKTHAAIYERSGGRLGAWLGLPTLLLSV
                     TGRKSGKTFATPLVYFKDGESYVVVGSDGAAKHDPQWWKNLQANPACVVRVGRRKLAA
                     KAALASGADRQRLWQQGKTVNPMWSKYQARTERELPVVVLTPTEG"
     gene            complement(1826..3016)
                     /locus_tag="VFG22_01360"
     CDS             complement(1826..3016)
                     /locus_tag="VFG22_01360"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HET6414913.1"
                     /translation="MKSTIGILTGGGDVPGLNPAIRAVTLRALREGHQVIGIRRGWGG
                     LVDIIPDKDADNSACVRVLTEDVVDKAGRTGGTFLHSSRTRPSTLPKDFVPPHLQDKY
                     KDDVNDITDEVLKNLDFLGIDCLIPIGGDDTLSYGAHLHQRGVPVVAIPKTMDNDVPG
                     TDYCIGFSTCVTRTIELTHKLRTSAGSHERFLVIEVFGRYAGYTALLPSMAGAASRCV
                     IPEHPFDMERLTELMVYDRNRNPSNYSVVLVSEGAQMASDSEMSFEGEEADMFGHRKL
                     GGIGDRVAAGLKELSPKYNNGRHVNVVSQRLGYLVRSGNPDALDSIAPMAFGNLALDL
                     VTSGTSGRLVSVHNGRYGSVPMETIIGTKKFVDVEKYYDTGRMRPKYTSFNGLPLFIM
                     ASDA"
     gene            complement(3061..4359)
                     /locus_tag="VFG22_01365"
     CDS             complement(3061..4359)
                     /locus_tag="VFG22_01365"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET6414914.1"
                     /translation="MIGVTRDARAGGKSARTDDKRHERWLARFIPRELVEDQLRSRAR
                     AFLLVRFTVVAAIFTLLFAPYFALYLKLPYTGLANVLYGVSLALTTVILNRTRSLRIA
                     ANWTLASALVVLLIQSWKLGGVSSLAYPWLASLPVAAMLLRGVKGGAVWTGIAVMGIV
                     GIGLADVAGWLPSGPALGVAAQGATTATIASLALALGALAWLAESRADELLDDLQQQK
                     DTFERRAVRDWLTGLANRSLLTECLVQSWERARRHGPRGALFFIDLNDFKLVNDQHGH
                     AAGDQVLREVALRLQDTIRASDLVGRIGGDEFALIVEGAESQNDVAVLADKVARSIEA
                     PIDVEGAIVQVGASIGIAFFPDRDCHFEERITLPSIPAKLNTTHGEVDHESVRRLLEK
                     ADTAMYTAKRKGLRFWIHKHEADETLRRTTALVSEPPPAA"
     gene            complement(4432..4887)
                     /locus_tag="VFG22_01370"
     CDS             complement(4432..4887)
                     /locus_tag="VFG22_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414915.1"
                     /translation="MQLPPCGLYRTTGPIGIVEPGRLVYFHNHGDPGPGVYVPKEWRY
                     NRALFEAKGQVLDDPGLVRFLVPLPREGFYRVLEAFHCCEKKCRLFEQDALLQLGYNG
                     DGEPILFTPELVDFMFAIPAKGWKTTLETLGNLSFLRVPETKRDASPTQ"
     gene            complement(4955..5419)
                     /locus_tag="VFG22_01375"
     CDS             complement(4955..5419)
                     /locus_tag="VFG22_01375"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HET6414916.1"
                     /translation="MGEEIERKFLVNDMTWRAFAEGVRYRQGFLSTVPERTVRVRVAG
                     PKAWITIKGKTVGARRAEFEYEVPSTDAERMLDTLCMRPLIEKVRYTLNVGRHSWEID
                     VFEGENAGLVIAEIELGSEDEAFEEPDWLGNEVTDDPRYFNSNLVANPYCNW"
     gene            complement(5450..5935)
                     /locus_tag="VFG22_01380"
     CDS             complement(5450..5935)
                     /locus_tag="VFG22_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414917.1"
                     /translation="MSYLEVQKIHDLNSLRHDPGEAARDEVLRMVLAITLDSDLAAWC
                     LGCRAEAPVVRWPSTLYRLLARAALESTAIWRRCALLLDRSLETSMAPYAAHSPAELA
                     EVFLEGRDSLSGDELAALLWCLLRQRSRSHDLIAERLGLELEVVAARRLHETRLPQGL
                     S"
     gene            6048..8096
                     /locus_tag="VFG22_01385"
     CDS             6048..8096
                     /locus_tag="VFG22_01385"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET6414918.1"
                     /translation="MRTRPGLLASALRLNAEQRAAVEHADGPLLVLAGAGSGKTRVIT
                     ARIARLLQSGMHPGSILAVTFTNKAANEMRERLGSSIGGEAANQLWLSTFHSFGVRFV
                     REEFKALGLGSRFTIFDQGDSLGVVRELLRTEAKADRQLDAQAILSRISLWKNALLEP
                     GKLRAKADEYDQAAASIYEAYETRLMDMHAVDFDDLVIKPVLLLRARPGIRSKWRSRF
                     EHILVDEFQDTNVAQLDLIRLLANERGNVCVVGDDDQSIYGWRGACVDNILDFERHFP
                     GAVVTKLEQNYRSRASILNVANSAIEKSSKRPYAKVLRPVRETGAPVRLCVLSEPQEE
                     AKFVASEIRKLRNEGTDPREIAVLYRSNTQAKRLEEELRIAGIPYRVFGGTKFFDRKE
                     IKDGIAYMRVALNPRDELSLRRIVNTPPRGLGAQSLHHIVEHAEAHRMSLLRALRSAD
                     RIEPLSDRAGASAIRLAEAIETAAKGFRGGDLGATAKGFLRAVGMIDDGGDRSPEAKR
                     RRANVDFLLRSVERLEARSGADREGLQQLLHHIALDQNDPEEPEAGMQVTLSSLHSAK
                     GLEFSVVFLIGCVEGVLPHARTTDPKITDASVADLEEERRLFYVGVTRARDLLYLTRH
                     KERVARGRLVKITPSRFLQGLPEADTETYQSSESRELERQEVADMAKALLDRLAARDA
                     "
     gene            complement(8104..9072)
                     /locus_tag="VFG22_01390"
     CDS             complement(8104..9072)
                     /locus_tag="VFG22_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414919.1"
                     /translation="MPIETSFERGLCALLSVWLLSVPSGQVFAEDLPAGEQAATPEQV
                     AAAEELESKPSMFAEGYPVRFARRPLVIDEGMVRADGRLTVGRVLGSSGVFSSLDIGG
                     AVSPIENLELGLSTELTGAVPAPGGIGLISAIFSPDASYGDIPVYARYQYAESETALA
                     GVDLVLVLPANTDFSITAGFPLRIVELFGLFTMDMTINIRYRNGDAYAAYISDPSKNT
                     ADFSFSGASITNITDHGYIEIGGGVALTNVGGGAGAKNVVELPFYLGGGYTYEGKVLV
                     DIFTQFGWQPLMLANQPPPLDTFNVTDNWYVTLGATVHTRPLFHKN"
     gene            complement(9201..10259)
                     /locus_tag="VFG22_01395"
     CDS             complement(9201..10259)
                     /locus_tag="VFG22_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414920.1"
                     /translation="MGEPFKADLRAHELPRETLVGLWNRTVDAHQKLGQTWADAVADK
                     YGRDVAEAIALEGWPLKKSGATPKQLFFDDLRFIVAATELKPEMLTVAERDQAEIPID
                     LRSDRSWFELLLAPARLLAANEKAPPGKPAWVGKPGRLRGYARGKGIDLGFSTLLKAW
                     RASQGKHPLKIDTDAFERKVTNEYDAKTLALIWNCAALAYMLGTDRWYTGVRARYGAD
                     AAQELEKDVWIDRGAAEHDLRIGQEAMGVTGDDVESLLRSFQFAPGEVGILDVEFELI
                     DANHGIMTHHACPALDRFEHFDDVRLKHCCDICLAGMPISGEMLNRKIECKPLKLPPR
                     RDANDIACRWEYRIRDQF"
     gene            complement(10259..11044)
                     /locus_tag="VFG22_01400"
     CDS             complement(10259..11044)
                     /locus_tag="VFG22_01400"
                     /inference="COORDINATES: protein motif:HMM:TIGR01458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01458 family HAD-type hydrolase"
                     /protein_id="HET6414921.1"
                     /translation="MRALLFDLDGVLYQGDRVIDGAVETVRWCDQRAIPHLFLTNTSS
                     RPRQSLIERLWELGLSVAAERVLTPAIAARDYLVREGLGPVALFVREATRADLEGLTL
                     LDERAESGAGSIIVGDIGNAWDFATLNRAFRLLMAEPQPALIALGMSRYAQAADGLVL
                     DVAPFIKALEHAADCEAIVMGKPARGFFDAALEMLGASASETVMVGDDLRSDVGGAQD
                     AGITGVLVRTGKFRPGDLEADVKPDVVLDSVADLPAWWDNRNL"
     gene            11145..11576
                     /locus_tag="VFG22_01405"
     CDS             11145..11576
                     /locus_tag="VFG22_01405"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET6414922.1"
                     /translation="MTLSLQEISDRLEIQDLVYEYSAIIDQKRFDDLRKVFTEDAHID
                     YSAFGAPVGGLDVIIRFLEQVMVMFPNHQHLNANVQIRVDGDAASGRVMCFNPQELAM
                     PDGKTHIFMCGLWYVDEYRRTEAGWRISRRVEEKSYVFNPP"
     gene            complement(11606..12898)
                     /locus_tag="VFG22_01410"
     CDS             complement(11606..12898)
                     /locus_tag="VFG22_01410"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HET6414923.1"
                     /translation="MKQKAKLEYGDQIYEMPIVEGSYQERAIDIRSLRKDTGLITLDS
                     GFMNTGATKSRITFINGEEGILLYRGYPIEQLAEHSSFVETSYLLIYGELPTSTEFNE
                     FSEMLTYHSMLHEDMRHFFDGFPSRAHPMAILSSMVSALTAYYPDSLEPDSHNIQHIA
                     RLLSKLRTIAAFSYQKAQGRPVAYPKNSLDYCGNFLHMMFSHPAEPTYEPDPELIKAL
                     NVLLILHADHEQNCSTSTVRLVGSSQASLYAAISAGISALWGPLHGGANQAVIEMLKE
                     IRAEGGDCNAFMRQVKDRERRLMEFGHRVYKNFDPRATILKKYADRLLDHLQIDDELF
                     DIAMQLEEVALSDDYFIERKLYPNVDFYSGIIYRAMGIPTEMFTVLFALGRIPGWIAH
                     WKEMMEDPDVRIGRPRQIYTGATRRDFTPRETRRPPAS"
     gene            13222..14076
                     /locus_tag="VFG22_01415"
     CDS             13222..14076
                     /locus_tag="VFG22_01415"
                     /inference="COORDINATES: protein motif:HMM:NF014077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HET6414924.1"
                     /translation="MFRRNRIRRVLLISLGLVMFPIAGCSSESSQTPSRPLRILVTND
                     DGVSALGIDAVVEALRNDPDNVIVVSAPAVNQSGSGDTTTPAPPPLEATETTTLSGYP
                     ATAVMGYPADSVLYALENLYPEEAPDVVLSGINQGQNVGNLDGVLSQISGTVGAAKTA
                     ACSGVPALASSQGSPKVGGDYDYEAGVVAVLAWLQANRAALLAGEASLEDITSINIPS
                     CDTGTIRGTVETVLATENPDGISLLGPQDCESDFQDPQDDMEAFFNGFISVTPVPSNS
                     SNTCDDLK"
     gene            14087..15484
                     /locus_tag="VFG22_01420"
     CDS             14087..15484
                     /locus_tag="VFG22_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414925.1"
                     /translation="MRIANASKFPSLAILSLVFAGCGSGDAPGGAAGSVGDAGSGGSA
                     GTSVLPGLGFVASSFGFYYPADAAAPIDGFDLDGRASSAASPSPGECPHDDFIGPDDE
                     PGIDYGFLRIIENEDLREDGKYVFGGFREGQLVDGVIGGATKNGSMTILLQVQGIDDP
                     NDDDEVTVQIFASEDSPALGTDNSVLRGATLSVHPDPRFHSGEVHGTVQDGVLTAGPI
                     DLRFPIDIMIVKDELLIHDSWLRITLGDGTFEGTVAGYWDVANIRSIIGKPTTDNGNA
                     ANFTIEQFDSAMAEFADGNYDSVSGLCTSFSTMFQFAGLQAFLVSESGPDGSGGSAGS
                     GGGGGVPTVIDQCVNDADQAALEALATPERSGPAIVGDIAADCPIAACGPEVGAVLSD
                     SSEAARNALGDCIATCIADRSGLSTACTGCYGTIAACSTAFCLAPCIADAGSQECADC
                     ALENCIDVNACTGLY"
     gene            15533..16456
                     /locus_tag="VFG22_01425"
     CDS             15533..16456
                     /locus_tag="VFG22_01425"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6414926.1"
                     /translation="MSLVSMFKGKGPSGFGYGSTAEDVTAGLDLSGRTVLLTGCNSGI
                     GKETLRVLAMRGAHVIAAARTLEKARAACDSVGGETTPVACELSDPASVRACAAEVIA
                     LGKPLDAIICNAGIMALPKLEQKHGYELQFFTNHIGHFILVTSLLDSLADKGRVVIVS
                     SDAHNGAPSEGIQFDNLSGERGYRPWASYGQSKLANLLFAKQLAKRLAGTGKTANSLH
                     PGVIHTNLTRSMNPIAKRALAIAGPLVLKSAAEGAATQCYLAVHPSVEGVSGEYFADS
                     NVSKSSSKGRDDALAEKLWKVSEKIAAEVTT"
     gene            16512..17999
                     /locus_tag="VFG22_01430"
     CDS             16512..17999
                     /locus_tag="VFG22_01430"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET6414927.1"
                     /translation="MSHSTAQVGTEARANGSHGSAAVVPEIDASKAEIERIFKLQLLH
                     QHSVARATARERKEKLKRLEKAILEHRQEIRDALYADFRKHPSEVDLTEIFPVTSEIK
                     HAARHLSKWMRPRRVKTPITLFGASSYIHYEPKGVVLIIAPWNFPINLTFGPLVAAIA
                     AGNCVMIKPSEHTPHASAVTKRIIAEVFDESEVAVVEGGIETSTSLLALPFNHIFFTG
                     APAIGKIVMEAASKHLASVTLELGGKSPTIVDETASVDAAAIRIAWAKYTNNGQICIA
                     PDYLFVHESKKEEFLSKLKENIESFYGLDAQKSESYARIVNHRHYQRLSRYIEDAKQK
                     GATVVYGGRTDASEDYIEPTVLTDVDPSSDVMTEEIFGPLLPVYSYKSLDEPIAMINS
                     KEKPLALYIYSKSKKNIQRIIDNTRAGGTCINHSAVHFFNSELPFGGSNNSGIGKGHG
                     FFGFEAFSNPRGILKQWSPISGLELMTAPFTKFKQLLIDLSIKYL"
     gene            18189..19967
                     /locus_tag="VFG22_01435"
     CDS             18189..19967
                     /locus_tag="VFG22_01435"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET6414928.1"
                     /translation="MTREAPCQYEPGSTRKRVLYVGSGLECFESLVADMSAAPRTERV
                     RSDLYPVLRSRGQELEWVPVWDTVGATGTLRSAYVNLLLVDLRWCENFENRVSEVRQL
                     LNALDHEDDVEQRYGFHRIVVMVSGPDASRLDDLIIELGAVGVRHVIRQDFREDVPAW
                     SSDEPFAERVLRSAVDLTGSRRIGKTAICASGGGITGIYFEMGVLKCLDDCLTNCSVN
                     DFDMMFGISAGAVVTSLLSVGYTADELMAGIASVPGGRVPPMSLSLLRLSHFNHEDMR
                     WRMSSAATTGIRAFWDTLRRRGGDTADSLFLEYTSLVGPPFHSSEFEVMLRRILQMSG
                     STNDFRKLPVELFVGASDQDARSHVLFGSEGYDHVPISKAVQASLSVNPAFASVPIDG
                     RYYEDGAITRTSNFGEAIGRNATLVVTLDPFVPYVSKLPGLAHRRGVLYNIDQDIRTI
                     SFTRFERARNAVLRKHPEVSSYTFLPSNTLRRLLSTNPMDHRPYLEIWRGAYLSALQR
                     IDQLCHRLRGDLAEQGMLLDTGMAEHVAEQLRTTTHLCFADFFPDRQVEIQKRPFMQP
                     SQLTSSPPPAADASDSLGSVPPSHGE"
BASE COUNT         3894 a         6282 c         6172 g         3865 t
ORIGIN      
        1 tggtccgaaa ggggaacgag ctcgtcaccc ccgacaggca ccacgtcttc gtcaaacgtg
       61 cggaccgtgg actgcaccgc gtcggatgtc agaccattga gcaagatgtg gtcgatccag
      121 accgagctcg ccgagtcggc cacgggattg tcttcgcaga aggtgcagag cggttcgtag
      181 tccgccgcga gcgcagcggg gaatgacgct tcgagcaagg ccagcgtggc ttcgccttct
      241 tcgctgatgc cctggtctgg ataggcgcgc cccgtgttga agtcgccgag gatgacagcc
      301 ggggagtcgc cgcttcggtc ttgcacgaat gcgatcaact tctgcgcttg aagatactgc
      361 tccgcttccc atcccgcagc gccggtctcc ccgttgccgt attggccggt gtacgggaag
      421 gtgaagtcgt cgaagatcgg cgtgaggtgg ttgcagtacg cgtccaaaga agcaccgttc
      481 ggcagagtca cggtcgcgcg aaggatcgtg cgccgattcc aggtgccagg catcacccaa
      541 ttttcttggt cgctcagcgg gtagcgcgag aggatcatca cgccattctg gccgtcgaag
      601 gcgaggtctt gattgacgac cgtcgggcaa ctgcttcgga tttcggcgaa cgtgctcgtc
      661 gggagttgcg ggacgacgca agaatagcat cgctgttgtt gcgcatcccc aaagaggagc
      721 gccgccaccg caccgacgca attcgtggac gcgcagctcg cgctagtcgt gcggccgtct
      781 tcgctgccgg ggatggtcga acagttctcc gctacgcagt cgaccgcctc gttcatctgg
      841 tccacgattg tgactccatc cgagacctca atatcgggac agggcacagt ggttggggct
      901 tggggtatct cgcccatctg atcggtgggg tcatccacgg gcgtgtcgag gtcgtcctcg
      961 aacagcgcgg agtacgggaa gttcccgacc gccatgtccc gaatgagctc tttgtcggcc
     1021 tgggtccaga cctcctgcaa acacaccacg tccgagtcca tccccgcgat cgcctcggcc
     1081 agcggctgcc ttcgttcggc ttcatacgga atgaaggctc cggccagagc cacgttgaag
     1141 gtgtcgatgg tgacgtcctc ggcttcgcgg tcgctcgagc tacaggcgcc aagcacgagc
     1201 aacggcacca cgaaaaagct aatgattcgc atctagtcct cctggtcggc gcacgccgat
     1261 gtgcaagctc tgaacgctgc ctcgagaatc tgtcacaaag ccgaaacaaa gggaagacgc
     1321 gcggtcgctc ggtggagctc cgcagccgca ggcgctgccc gacgggtgtg gctagccttc
     1381 cgtcggcgtg agcacgacca ccggcagctc gcgctcggtt cgcgcctggt acttcgacca
     1441 catcgggttc acggtcttgc cctgctgcca gaggcgttgg cgatcggcgc ctgatgcaag
     1501 cgcagccttt gccgcgagct tcctccgacc gacgcgaacc acgcatgctg gattcgcctg
     1561 caggttcttc caccattgag ggtcgtgttt cgcagctccg tccgagccga ccaccacata
     1621 gctctcgccg tctttgaaat agaccaaggg cgtcgcaaac gttttgccgg acttccgccc
     1681 agtaacggag agaagcaagg tcggcaatcc gagccaagcg ccgagacggc ctccgctacg
     1741 ctcgtagatc gcggcatgcg tcttgaacat cgacttgagc gcacgcgccc gaaggtgcct
     1801 gtagcgcgag gccatcgacg gacccttacg cgtcgctcgc catgatgaaa agcggcaaac
     1861 cgttgaagga cgtgtacttc ggtcgcattc ggccggtgtc atagtacttt tcgacatcga
     1921 cgaacttctt cgttccgatg atcgtttcca tcggcacact gccgtagcgg ccattgtgca
     1981 cggagacgag tcgtcccgag gtaccggacg tgaccaagtc caacgcgaga ttgccaaagg
     2041 ccatcggcgc aatcgagtcg agcgcatcgg ggttaccgga gcgcaccaaa tagccgaggc
     2101 gttggctcac cacgttgacg tgccgaccgt tgttgtactt gggcgagagc tctttgagcc
     2161 ctgctgcaac gcgatctccg atgccgccga gctttcggtg accgaacatg tcggcctctt
     2221 cgccctcgaa cgacatctcg gaatcgctcg ccatctgggc gccctcggac acgagcacca
     2281 ccgagtagtt gcttgggttt cgattgcggt cgtagaccat cagctccgtc agccgctcca
     2341 tgtcgaaggg gtgctctgga atgacacatc gactcgcagc gccagccatg ctcggcaaga
     2401 gcgccgtgta cccggcatac cgaccgaaga cctcgatgac gaggaatcgc tcgtgcgatc
     2461 ctgcggacgt gcgtagcttg tgcgtcagct cgatcgtgcg ggtgacgcag gtgctgaacc
     2521 cgatgcagta gtccgttccc ggcacgtcgt tgtccatcgt cttcggaatg gcgaccaccg
     2581 gaacgcctcg ctggtgaagg tgcgccccgt agctcagcgt atcgtcgccg ccgatgggaa
     2641 tcagacaatc gatgcctagg aagtccaggt tcttcaggac ctcatcggtg atgtcgttga
     2701 catcgtcctt gtacttgtct tgcagatgtg gcggtacgaa gtctttgggc aaggtgctcg
     2761 ggcgggtcct cgagctgtgc aagaaagtac caccggtccg ccccgctttg tcgaccacgt
     2821 cctcggtgag tacgcgcacg cacgcgctgt tgtcagcgtc cttgtccgga atgatgtcga
     2881 ccagcccacc ccagccgcgt cggatcccga tcacctggtg accttcgcga agtgctcgga
     2941 gcgtcaccgc acgaatggct ggattgagac ccggcacatc gccgccgccc gtgagaatcc
     3001 cgatcgtaga tttcatgggg caactctagc gcaagccgcg agcctggcta gaggttctca
     3061 ctaagcggca ggtggcggct cgctcacaag ggcggtggtt ctccgcagcg tctcgtccgc
     3121 ttcatgtttg tgaatccaga atcgtagccc ttttcgcttc gcggtgtaca tcgcggtgtc
     3181 cgccttctcg aggagtcggc gcaccgactc gtggtcgacc tcgccgtgtg tcgtatttag
     3241 tttggcgggg atgctcggaa gggtgatccg ttcctcaaag tgacaatctc gatccgggaa
     3301 gaacgctatt ccgatactcg ccccgacctg gacgatcgcc ccctcgacgt cgatcggagc
     3361 ttcgatggac cgcgctactt tgtcggcgag cacggccacg tcgttttggc tctcggcgcc
     3421 ctcgacgatc agcgcaaact cgtccccgcc gattctgccg acgaggtccg aggcgcggat
     3481 ggtgtcctgc aggcggaggg cgacttcgcg caggacctgg tcccccgccg cgtgcccgtg
     3541 ctggtcgttg accagcttga aatcgtttag gtcgatgaag aacagtgcgc cgcgagggcc
     3601 gtgccgccgc gcgcgctccc agctctgaac caggcattcc gtgagcagtg accgatttgc
     3661 aagaccggtc agccagtcac gcactgcgcg tcgttcaaac gtgtccttct gctgctggag
     3721 gtcatccaag agctcgtctg cccggctttc ggccaaccaa gccagggctc cgagcgcaag
     3781 cgccagcgaa gcgatcgtgg cggtggttgc cccctgggct gccacgccca gtgccggccc
     3841 gctcgggagc caacccgcga catcggcgag gccgattccc acgatcccca tcaccgcgat
     3901 cccagtccaa acggctccgc ccttgacgcc gcgaagaagc atcgccgcca cgggcaggga
     3961 ggccagccac gggtaggcga gactcgagac gccaccgagc ttccagctct ggatgagcaa
     4021 gaccacgaga gcagatgcga gggtccagtt ggccgcgatt cgaagcgagc gcgtgcgatt
     4081 caggatgacc gtcgtcagcg ccaggctgac tccatagagc acgttggcca ggcccgtgta
     4141 cggaagcttc aagtagagag cgaaataggg cgcgaaaagg agggtgaaga ttgcagcaac
     4201 gaccgtgaac cgcacgagca ggaacgcgcg cgcgcggctc cgaagctgat cctccacgag
     4261 ctcgcgaggg atgaatcgag ccagccagcg ctcgtgccgc ttgtcatcag tccgtgcgct
     4321 cttgccaccc gcacgcgcgt ctcttgtgac cccgatcatt ctaagtttca gatgatcagt
     4381 ataagcagct ggttccggga gcgaaaagcg agctagctca cgctcggacg tctactgcgt
     4441 gggactggcg tcgcgcttgg tctcaggcac gcgcaagaag ctcagattcc caagtgtttc
     4501 tagcgtcgtc ttccagccct ttgcgggaat cgcgaacatg aagtccacca attcaggcgt
     4561 gaatagaatc ggctcgccat cgccgttgta accgagctga agcagcgcgt cttgctcgaa
     4621 gagccgacac ttcttttcgc aacaatgaaa ggcttcgagc acccgataga agccttcgcg
     4681 aggaagcggc acaagaaatc gcaccaggcc cggatcatcg agcacttgcc ctttcgcctc
     4741 gaagagcgcg cggttgtatc gccactcctt cgggacgtac acgcctggcc cgggatctcc
     4801 gtggttgtgg aaatagacca ggcgtccggg ttccacgatt ccgatcggtc cagtcgtccg
     4861 gtacaagcca cagggcggaa gctgcatacc gcagacctaa accagcgacc cgcgcctggc
     4921 aatgcaaggc agctcgagct ctgaagagct agcgctacca gttgcaatac ggattggcga
     4981 cgagattgga gttgaaatag cgcggatcat ccgtgacttc gtttcccaac cagtcgggct
     5041 cctcgaatgc ttcgtcttcg ctgccgagct cgatctccgc gatgactaga ccggcgtttt
     5101 caccttcgaa gacgtcgatc tcccagctat gccgaccgac gttcagcgtg tagcggactt
     5161 tttcgatcag cgggcgcatg caaagcgtgt cgagcattcg ttcggcgtcg gtcgatggaa
     5221 cctcgtattc gaactccgcg cggcgcgcgc caaccgtctt tcccttgatg gtgatccaag
     5281 ccttgggtcc ggcgacacgc actcgaaccg tccgctccgg caccgtcgag aggaagccct
     5341 gacggtaccg gacaccctcg gcgaaagcgc gccacgtcat atcgttgacc agaaacttcc
     5401 gctcgatctc ttcgcccatg ctgccctgtc gacttcgccc ttctaacatt cacgagagac
     5461 cctgtggcaa gcgcgtttcg tgaaggcgcc gcgctgctac gacctcgagc tcgagaccga
     5521 gtcgctcggc aatcaggtcg tgtgaacggg agcgctgacg cagcaagcac caaagcagtg
     5581 cagccagctc gtctccgctc agcgaatccc ggccttcgag aaagacctcg gcgagctcgg
     5641 ccggggagtg agcggcgtag ggagccattg aggtctcgag ggatcgatcg agcaggagcg
     5701 cgcagcgccg ccagatggcg gtcgattcga gcgcggctcg agcgagcaag cggtagagcg
     5761 tagagggcca gcgcacgacc ggggcttcgg ctcgacatcc gaggcaccag gcagccaagt
     5821 cggagtcgag cgtgatcgcc agcaccattc tgagcacctc atccctggca gcctctccgg
     5881 ggtcatgccg caagctgttg agatcatgaa ttttttgaac ttccaaatag ctcatacatc
     5941 cctgataatg aaaatcacta tcaataaagt acggtagatc gaaggccccg tcaacggata
     6001 tctctcctgg acgagctctt tgctcgcttt cgtccggatg tccttgaatg agaacacgcc
     6061 ccgggctact tgcctcggcc ttgcgattga atgcagagca gcgggcggcg gtggagcatg
     6121 cagacgggcc cttgctggtt ctcgcgggag cgggctccgg gaaaacccgc gtcattaccg
     6181 ccaggatcgc gcgtctgctg caaagcggaa tgcatccggg ctcgatcctg gccgtcacct
     6241 tcaccaacaa ggcggccaac gagatgcgag agcgtctcgg atcgagcatc ggcggggagg
     6301 ctgcgaatca gctctggctc tccacgtttc acagcttcgg tgtccgcttc gtgcgcgagg
     6361 aattcaaagc gctcgggcta ggctcacgct tcacgatttt cgaccagggg gatagcctcg
     6421 gcgtcgttcg ggagctcttg aggaccgagg cgaaagcgga ccggcagctc gatgcacagg
     6481 ccatcctctc tcggatctcg ctctggaaga acgcgctcct cgagccgggg aagctacgcg
     6541 ccaaagcaga cgagtacgac caagccgccg cgagtatcta cgaagcttac gagactcgcc
     6601 tcatggatat gcatgcggtg gatttcgacg acttggtgat caagcccgtc ctccttcttc
     6661 gcgcacgccc agggattcga agcaagtggc gtagtcgatt cgagcacatt ctcgtcgacg
     6721 agttccagga cacgaacgtc gcgcagctag acctcatccg acttctcgcg aacgaacgcg
     6781 gcaacgtctg cgtcgtgggt gacgatgatc agtcgatcta tggctggcgc ggcgcgtgcg
     6841 tcgacaacat cctcgatttc gagcggcatt tcccaggtgc ggtcgtcacc aagctcgagc
     6901 agaactaccg gtcgcgcgct tcgattctga acgtggcaaa ttcagcgatt gagaaatcga
     6961 gcaagcgtcc ctacgcgaag gtgctgaggc ccgtgcgaga gaccggcgca cccgtacggc
     7021 tctgcgtgct ttcggagccc caggaggaag cgaagttcgt ggcttccgag atccgcaagc
     7081 tcaggaatga gggcaccgac ccacgtgaga ttgcggtgct ctatcgctcc aatacgcagg
     7141 ccaagcgcct cgaagaggag cttcggatcg ctggcatccc gtaccgcgtg ttcgggggaa
     7201 ccaagttctt cgatcgaaag gagatcaagg acgggattgc gtacatgcgc gtggccttga
     7261 atccccgcga cgagctttcg ctccgccgaa tcgtgaacac gcctccgcga gggctcggcg
     7321 cacaatcact tcatcacatc gtcgagcacg ccgaggcgca tcgaatgagt ctcttgcgtg
     7381 cattgagaag cgccgaccgg atcgagccgc tctcggaccg agccggtgct tcggcgattc
     7441 gattggccga ggcgatcgaa acagccgcaa agggatttcg tggaggggac ctcggcgcaa
     7501 ccgccaaggg cttcttgcgt gcggtcggaa tgatcgacga cggcggcgat cgcagtcccg
     7561 aggccaagcg acgccgtgcc aacgtcgact tcctcttgcg aagcgtcgag cgcttggagg
     7621 cacgcagcgg tgcggatcgt gagggcttgc aacagcttct gcaccacatc gcgctcgatc
     7681 agaacgaccc ggaggagccc gaagccggca tgcaggttac gctctcgtcc ttgcattcag
     7741 caaagggcct cgagttctcg gtggtcttct tgatcggctg tgtcgagggt gtgctgcctc
     7801 atgcgagaac gaccgacccc aagatcaccg acgcctcggt ggctgacctc gaggaagagc
     7861 ggcgattgtt ctacgtgggc gtgacacgcg cgcgcgacct gctctacctc acgcgccaca
     7921 aggagcgcgt tgctcggggg cgtctggtga agatcacgcc gagtcgattc cttcaagggc
     7981 ttccagaggc ggacaccgag acctaccaga gcagcgagag ccgcgagctc gaacggcagg
     8041 aggtggccga catggccaag gcactgctcg atcggctcgc agcgcgcgat gcctgagcgg
     8101 cgactagttc ttgtggaaga gcggccgagt gtggacggtc gctccgagag tgacgtacca
     8161 gttgtccgtg acgttgaagg tgtccagggg tgggggctga ttcgcgagca ttagaggctg
     8221 ccatccaaac tgcgtgaaga tatcgacgag cactttgcct tcgtaggtat atccaccgcc
     8281 caagtagaac ggaagctcca cgacgttctt cgctcccgcg ccgccgccca cgttggtcag
     8341 cgcgacacct ccgccgattt cgatgtaacc gtggtcggtg atgttggtga tgctcgcgcc
     8401 cgagaaactg aagtctgccg tgttcttcga aggatcggaa atgtaggcgg cgtacgcgtc
     8461 gccgttccgg taccgaatgt tgatggtcat gtccatcgtg aagaggccaa agagctcgac
     8521 gatgcggagg ggaaagccgg ccgtgatcga gaaatcggta ttcgccggca agaccaggac
     8581 caggtcgacg cccgcgagcg cagtctcgct ctcggcgtac tgatagcggg cgtagaccgg
     8641 gatgtcgccg tagctcgcgt ccggcgaaaa gatggcgctg atgagcccga taccacccgg
     8701 agcggggact gcaccggtga gctccgtgct caaacccagc tcgagatttt cgattgggct
     8761 gaccgccccc ccgatgtcca gcgaagaaaa gacaccggag cttccaagca cgcgaccaac
     8821 cgtgagtctg ccatcggcgc gaaccatgcc ttcgtcgatg accaagggcc gcctcgcgaa
     8881 gcgaaccggg tacccctctg cgaacatcga cggcttcgac tccagctctt cggccgctgc
     8941 gacttgctca ggggtcgcgg cctgctcgcc cgccgggagg tcctcggcaa acacctggcc
     9001 agaagggacg ctcaagagcc acacgctcag cagcgcgcaa aggcctcgtt caaaggatgt
     9061 ctcgatcggc attccggcga ctctagccgt tcatctggat cggggtcgag gtttttcgac
     9121 gcttcgttct gggcaagctc ttgccacgca aacgacttga tccactcggc cgtgagccag
     9181 ggcgcggctt ggctggccgc tcagaactga tcgcggatac ggtactccca gcggcaggcg
     9241 atatcgttcg catcccgcct gggcgggagc ttcagaggct tgcactcgat tttccggtta
     9301 agcatctccc ccgagatggg cattccggcc agacagatgt cgcagcagtg cttcaatcgc
     9361 acgtcatcga agtgctcgaa gcggtcgagc gccggacagg catggtgcgt catgatccca
     9421 tgattcgcat cgatgagctc gaactccaca tcgagtatgc cgacctcgcc gggcgcgaac
     9481 tggaagctgc gaagaaggct ttcgacatcg tcaccggtga cgcccatcgc ctcctggccg
     9541 attcgcagat cgtgctcggc cgcgccacgg tcgatccaga cgtccttctc gagctcctgg
     9601 gccgcatcgg ctccgtagcg tgcccggacg cctgtgtacc agcggtcggt tcccagcatg
     9661 taggcgaggg cagcgcagtt ccaaatcaac gccaaggtct tcgcatcgta ttcgttggtg
     9721 actttccgct cgaaggcgtc ggtgtcgatc tttagcggat gcttcccctg agaagcccgc
     9781 caagccttga gcagagtcga gaagccgagg tcgatgccct tgccccgagc gtatccgcgc
     9841 agccttcccg gtttcccgac ccaagccggt tttcccgggg gcgccttttc gtttgccgcg
     9901 agcaatcgcg ccggtgcgag caggagctcg aaccaggagc gatcgctgcg caggtcgatt
     9961 gggatctccg cctgatcgcg ctcggcgacg gtgagcatct ccggtttgag ctcggttgcc
    10021 gcgacgatga acctcagatc gtcgaaaaag agctgcttcg gcgtcgcacc tgacttcttg
    10081 agcggccagc cctcgagggc gatcgcttcg gcaacatcac ggccatattt gtcggcgacc
    10141 gcatctgccc aggtctgacc gagcttctga tgggcatcca ccgtgcgatt ccagagccca
    10201 accaaggttt ccctcggcag ctcgtgtgct ctcagatcgg ctttgaaggg ctcgcccatc
    10261 acagattccg attgtcccac caagcgggca aatcagccac cgaatccagg actacgtccg
    10321 gtttaacgtc tgcttcgagg tctcccgggc gaaatttccc cgtccgaacc aagaccccgg
    10381 tgatccccgc atcctgagcg cccccgacat cgctccgaag atcgtcaccg accatcaccg
    10441 tttcgcttgc cgaggcaccg agcatctcca gagcagcgtc aaagaagccc cgggccggct
    10501 tgcccatgac gatcgcctca cagtccgcag cgtgctccag tgccttgatg aagggagcga
    10561 catcgagaac caggccatcg gcggcctgcg catatcgtga cattccgagc gcgatcagcg
    10621 ccggctgcgg ctctgccatc aagagacgaa acgcgcgatt caaggtggca aaatcccatg
    10681 cattcccaat gtcgcccacg atgatacttc ccgcgccgga ctcggcccgc tcgtcgagga
    10741 gggtgagccc ttcgaggtct gcgcgtgtcg cttcgcgaac gaatagagca accgggccca
    10801 gaccttcgcg cacgaggtag tcgcgcgccg cgatcgctgg cgtgaggacc ctctcggctg
    10861 cgaccgagag tccgagctcc cagagccgct cgatcagtga ttgacgtggc ctcgagctgg
    10921 tgttggtcag gaacaagtgc ggaatggcgc gctgatcaca ccaacgcacc gtctcgaccg
    10981 cgccatcgat gacgcggtcg ccttgataca gcacaccgtc gaggtcgaag agaagtgccc
    11041 gcatgctcac ctcccccggt ctcatcaatc ataacaaaat gcaacgaaag agggcgtgaa
    11101 gaatacgcgc gtcggctatg ctccctcgga ccaaaggagc tgagatgacc ttgtcgttgc
    11161 aggagatctc cgaccgcctg gagattcaag atctagtcta cgagtactct gcgatcatcg
    11221 accagaagag gttcgacgat cttcgcaagg tgttcaccga ggacgcgcac atcgactaca
    11281 gcgcctttgg tgcgcccgtc ggcgggctcg atgtgatcat cagattcctc gagcaggtca
    11341 tggtgatgtt tccgaaccac cagcacctca atgccaacgt gcagatccgt gtcgacggag
    11401 atgccgcttc cggtcgcgtg atgtgcttca acccccaaga gctcgcgatg ccggatggaa
    11461 agacacacat cttcatgtgc ggcctttggt atgtcgatga gtacaggcgg accgaggcgg
    11521 gttggcgtat cagccggcgc gtcgaagaga agagctacgt cttcaatccg ccctaaaacc
    11581 actgccgtga gttgcccgga gcgctctagg acgccggtgg gcgacgggtt tctctcgggg
    11641 tgaaatcgcg ccgtgtggcg cccgtgtaga tctggcgcgg tcgtccgata cgcacgtccg
    11701 ggtcttccat catctctttc cagtgtgcga tccagccagg gatgcgaccg agtgcgaaga
    11761 gcaccgtgaa catctcggtg ggaatgccca tcgcgcggta gatgatgccg ctatagaaat
    11821 cgacgttggg gtagagcttc cgctcgatga agtaatcgtc cgagagagcg acctcttcga
    11881 gctgcatagc gatgtcgaag agctcgtcgt cgatttgcag gtggtccagt aggcggtctg
    11941 cgtacttttt gaggatcgtc gcgcgaggat cgaagttttt gtacacgcgg tgaccgaatt
    12001 ccatgagccg acgctcgcgg tccttgacct gtctcatgaa ggcgttgcag tcgccgcctt
    12061 cggcccggat ctctttcagc atttcgatga ctgcctggtt ggcgccgcca tgcagcgggc
    12121 cccacagggc tgaaattccc gcactgatcg ccgcgtagag gctcgcctgg ctcgatccca
    12181 ccagtcgaac ggtcgatgtc gagcagttct gctcgtgatc ggcgtggaga atcagcagca
    12241 cgttgagtgc cttgatcagc tccgggtcgg gctcgtacgt gggctccgcc gggtgcgaga
    12301 acatcatgtg gaggaagttc ccgcagtagt cgaggctgtt ctttgggtag gcgaccggtc
    12361 gtccctgcgc cttctgatag ctgaatgcgg cgatggttcg aagcttggag agcagccgtg
    12421 cgatgtgttg aatgttgtgc gagtccggtt cgaggctgtc cgggtagtac gctgtgaggg
    12481 cgctgaccat tgaggagagg atcgccatcg gatgggcccg cgaagggaag ccatcgaaga
    12541 agtggcgcat gtcctcgtgc aacatgctgt ggtacgtgag catctccgag aattcgttga
    12601 actcggtgga ggtcggcagc tccccataga tcagcaagta gctcgtctcc acgaaggacg
    12661 aatgctcggc cagctgctcg atcggatatc cgcggtagag cagaatgccc tcttcgccat
    12721 tgatgaacgt gatccggctc ttggtagctc cggtgttcat gaagccggag tcgagggtga
    12781 tcagcccggt gtctttgcgc aggcttcgaa tgtcgatcgc gcgttcctgg tatgagcctt
    12841 cgacgatcgg catctcgtag atctggtcgc cgtattcgag ttttgctttt tgtttcatga
    12901 attcagcccg agttctagcg tgggcgtggg ccctgggcaa cgtcgctccc attcaggttt
    12961 ccggcctgtt tcttcgggca aaactcggtt tccgtcgctc tttcgtgcac gcccgcgctg
    13021 ctcggcgtgc gcgcctggtc gaagaagggc ctcgaatcgg ttgccaacga cctcgtcggc
    13081 tgcagcgcca ctggggcctg gagcacacca tacggtacca gcagagatgg gtagtggggg
    13141 cgtaccttga tctgcggtct gtgtaagctg cgcacccgcc agctattgcg cagctccaac
    13201 cttgtttcta gtatcgcgcg catgtttcga agaaatcgga tccggcgcgt gctccttatt
    13261 tcgcttggcc tcgtgatgtt cccgatagcg ggctgcagca gcgaatccag tcagacaccc
    13321 tctcgccctc ttcgaattct cgtcaccaac gatgacggcg tgagcgcgct gggaatcgac
    13381 gcagtcgttg aggccctcag aaacgacccc gacaacgtaa tcgtggtttc ggcgccagcg
    13441 gtcaaccaga gcggcagtgg tgacaccacc acgccggctc ccccaccgct cgaggcaact
    13501 gaaacgacga ccttgagcgg ctatcccgcg accgcggtga tgggctaccc ggccgattcg
    13561 gttctctacg cgctcgagaa tctctacccc gaggaggcgc cagacgtggt gctctccggg
    13621 atcaaccaag ggcagaacgt tggcaacttg gatggcgtgc tttcccagat ttcgggcacc
    13681 gtaggtgctg cgaagacggc tgcttgttcg ggcgtcccag cgctcgcatc ttcccagggc
    13741 agcccgaaag tcggcggcga ctacgactat gaggcaggcg tggtcgcggt cttggcctgg
    13801 ctccaagcca atcgagccgc gcttcttgcg ggtgaggctt cgctagaaga catcacgagc
    13861 atcaacatcc cgagctgcga tacggggacg attcgcggga ccgtcgagac cgtcttggcg
    13921 accgagaacc ccgatggcat ctccctcctt ggtccccagg actgcgagtc ggacttccag
    13981 gatcctcagg acgacatgga agctttcttc aacggcttca tctccgtcac gccggttccc
    14041 agcaacagca gcaacacctg cgacgacctc aagtgaacga cgctcgatga gaatcgcgaa
    14101 cgccagcaag tttccctccc tggccatcct gagcctggtc ttcgccggct gcggtagcgg
    14161 cgatgccccg ggcggggccg ccggcagcgt tggagacgcc ggcagcggcg gttccgccgg
    14221 cacgagcgta ttgcccggcc tcgggttcgt cgcttcgtcg ttcggattct actaccccgc
    14281 tgatgccgca gccccgatcg acggattcga tctcgacggt cgcgcttcga gcgcggcatc
    14341 gccttcccct ggtgagtgcc cccacgatga tttcatcggg cccgacgacg aacccggtat
    14401 cgactacggc tttctccgca tcatcgagaa cgaggatctt cgtgaagacg gcaagtacgt
    14461 gttcggcggg tttagggagg ggcagctcgt cgacggggtg atcggcggcg cgaccaagaa
    14521 tggctcgatg acgatactgc tgcaggttca gggtatcgac gatcccaacg acgacgacga
    14581 ggtgaccgtc cagatcttcg cgagcgagga ttcacccgca ctcggcaccg acaactcggt
    14641 gcttcgaggg gcgacgctca gtgttcatcc cgatcctcgt ttccacagcg gggaagtgca
    14701 tggtaccgtt caggacggcg tgctcaccgc cggcccgatc gatcttcgat ttccaatcga
    14761 catcatgatc gtgaaggacg agctcttgat ccacgacagc tggctgcgga ttaccctcgg
    14821 cgacggcacg ttcgagggca ccgttgctgg ctattgggac gtggcgaaca tccgcagcat
    14881 catcggcaaa cccaccacgg acaatggcaa tgcggccaac ttcacgatcg aacaattcga
    14941 ttccgcgatg gccgaattcg ccgacggcaa ctacgactcc gtatccggtc tctgcacctc
    15001 cttcagcacg atgtttcagt ttgccgggct gcaagctttc ctggtgagcg agagcgggcc
    15061 cgacggatcg ggcggaagcg ctggctctgg tggcggtggg ggcgtgccga cggtcatcga
    15121 ccaatgcgtg aacgacgcgg atcaggctgc gctcgaggct ctagccaccc cggaacgaag
    15181 cggacctgcg atcgtcggcg acatcgccgc agattgcccg atcgcagcct gtggccccga
    15241 agtcggggcc gtgctctcgg acagctccga ggcagcgcgt aatgcgctcg gcgattgcat
    15301 cgccacttgc attgccgacc gaagtggcct ttcgaccgcg tgcactggct gctacggcac
    15361 catcgcggcc tgctcgacgg ctttctgcct tgcgccctgc atcgcggatg cgggctctca
    15421 agagtgcgcc gattgcgcgc tcgagaactg tatcgacgtc aacgcctgta cagggctcta
    15481 ctgagcgcta gaaacccgga tagaagtttg cattcgccga aggggtgatt cgatgtctct
    15541 agtctccatg ttcaagggca aaggtccgag cgggttcggc tacggaagca ccgccgagga
    15601 cgttaccgcg ggtctcgatc tgagcgggcg gacggtcttg ctgaccggct gcaactccgg
    15661 catcggcaag gagacgctcc gagtgctcgc catgcgtgga gcgcacgtca tcgcggccgc
    15721 gcgcacgctc gaaaaggcca gagccgcttg cgattcggtg ggcggtgaaa ccacgcctgt
    15781 cgcgtgcgag ctctccgatc cggcttcggt gcgagcctgc gctgccgagg tcatcgccct
    15841 tggaaagccc ttggacgcga tcatctgcaa tgcgggcatc atggcgctac cgaagctcga
    15901 acagaagcac ggttacgagc tccagttctt caccaaccac atcggccact tcatcttggt
    15961 gacttcgctt ctcgatagcc tcgccgacaa ggggcgtgtg gtgatcgtca gcagcgacgc
    16021 gcacaacggc gcgccaagcg agggcatcca gttcgacaat ctcagtggcg agcgagggta
    16081 ccggccgtgg gcgagctatg gacagtcgaa gctcgcgaat ctgctcttcg cgaagcagct
    16141 ggcgaagcgg cttgcgggca ccggaaagac agccaacagc ctgcatccgg gcgtgattca
    16201 cacgaacctt acccggagca tgaacccgat cgccaagcga gcgctcgcga ttgcgggtcc
    16261 actggtgctc aagagcgcgg ccgaaggtgc ggctactcag tgctatctcg cggtgcatcc
    16321 gagcgtcgaa ggcgtgagcg gcgaatactt cgccgactcc aatgtctcga agtcgagcag
    16381 caaagggcgt gacgacgcac tggcggaaaa gctttggaag gtctccgaga aaatcgctgc
    16441 cgaggtcacc acttgacaag aagaagcagc accgagcatt gtccgttttc tagtttacga
    16501 aagttgaaaa gatgtcgcat tccacagctc aggtaggcac cgaggcgcgc gcaaacggct
    16561 cacacggatc tgctgccgtc gttccagaga tcgacgcgag caaggctgag atcgagcgta
    16621 ttttcaagct ccagctgctc catcagcact cggtggcaag ggccacggcg cgggagcgca
    16681 aagagaagct gaagcggctc gagaaagcga tcttggagca ccggcaagag atccgtgacg
    16741 cgctctacgc ggacttccgc aagcacccga gcgaggtcga tctgacggag atctttccgg
    16801 tcaccagcga gatcaaacac gcggctcgcc acctctccaa gtggatgcgt ccgcgccgcg
    16861 tcaagacacc gatcacgctg ttcggagcga gctcctacat ccactacgag cccaagggcg
    16921 tggtgctgat catcgctccc tggaacttcc cgatcaatct caccttcggc cctctggtcg
    16981 ctgcgatcgc agcgggcaat tgcgtgatga tcaagccatc tgagcacacc ccgcacgctt
    17041 cggcggttac caaacgcatc atcgccgagg tgttcgacga gagcgaagtc gccgtcgtcg
    17101 aaggcggcat cgaaacctcg acatcgcttc tcgcgctgcc gttcaaccac atcttcttca
    17161 cgggcgctcc ggctattgga aagatcgtca tggaagccgc gtcgaagcac ctggcatcgg
    17221 tgacgctcga gctcggtggc aagtcaccga ccatcgtcga cgaaaccgcc agtgtcgacg
    17281 ccgctgcgat acggatcgcc tgggcgaagt acacgaacaa cggtcagatc tgcatcgcac
    17341 cggactatct cttcgtgcac gagagcaaga aggaggagtt cctcagcaag ctcaaggaaa
    17401 acatcgagtc gttctacggg cttgacgcgc agaagtccga atcgtacgcc cgaatcgtca
    17461 accaccggca ctatcaacga ctatcccgct acatcgaaga cgccaagcaa aagggggcga
    17521 ccgtggtcta tggcggtcga accgatgcga gcgaggacta catcgagccg acggtcttga
    17581 ccgacgtcga tccgagctcc gacgtcatga ccgaagagat cttcggcccg cttttgccgg
    17641 tctacagcta caaatcgttg gacgagccga tcgcgatgat caactccaag gaaaagcccc
    17701 tcgccctcta catttacagc aagagcaaga agaacatcca acgcatcatc gacaatacgc
    17761 gcgccggggg cacctgcatc aaccacagcg cggtgcactt cttcaatagt gagctgccgt
    17821 ttggtggctc gaacaatagc ggcatcggaa agggccatgg tttcttcgga ttcgaggcct
    17881 tttcgaatcc ccgtggcatt ctcaaacagt ggtcgccgat tagcgggctc gagctcatga
    17941 ccgcgccgtt cacgaagttc aagcagctgc tcatcgacct cagcatcaaa tacctctgag
    18001 cggatggcct acgacgagaa gcttgccgat cgagtgcgcg cgagagatgg acttcacggg
    18061 ccgagccatg aaaggcatgg tatacgtcgg cgcgcgcggt cgcgggcgtg cgccgagctt
    18121 gaaggcctgg gtagagcgag gtctcgttta cgccaggtcg cttcccccaa ggacttgaat
    18181 ctagactcat gacgcgtgag gcaccctgtc agtacgaacc cggcagtacg aggaagcgcg
    18241 tgctctacgt cggctcaggc ctcgagtgct tcgagtcgct cgtcgctgac atgagcgcgg
    18301 caccgcgcac cgagcgggtg cgttcagacc tctatcctgt gctccgcagt cgcggccagg
    18361 agctcgagtg ggtgcccgtc tgggataccg tgggagcgac cggcaccctg cgctccgcgt
    18421 acgtgaacct tttgctagtc gacctgcgct ggtgcgagaa cttcgaaaac cgtgtctccg
    18481 aggttcgcca gctcctgaat gcgctcgacc acgaagacga cgtcgagcag cgctacggct
    18541 tccaccgcat cgtggtgatg gtttcaggtc cagacgcgag ccgactcgac gacctgatca
    18601 tcgagctcgg cgctgtcggg gttcgacatg tcattcgtca ggacttccga gaagacgttc
    18661 ccgcttggtc gagtgatgag ccattcgcag agcgggtgct tcgcagcgca gtcgatctca
    18721 ccggatcacg ccgcataggc aagacggcga tctgcgcctc tggcggtggc atcaccggga
    18781 tctacttcga aatgggcgtg ctcaagtgcc tcgacgattg cctcaccaac tgcagcgtaa
    18841 acgacttcga catgatgttt ggcatcagcg caggggcggt cgtgacgagc ctcttgtcag
    18901 tcggctacac cgcggacgag ctcatggcgg gtatcgcgag cgtacccggc ggccgcgtgc
    18961 cgcccatgtc gttgagcctt cttcgactgt cgcacttcaa tcacgaggat atgcgttggc
    19021 gcatgtccag cgccgcaacg accggaatcc gtgcgttttg ggacaccttg cgcagacgcg
    19081 gcggcgacac ggccgacagc ctctttctcg aatacacgag cctggtcggg ccaccgttcc
    19141 actcctcgga attcgaggtg atgctgcgcc gcattctgca gatgtccggg agcacgaacg
    19201 atttccgcaa gctcccagtc gagctcttcg ttggcgcctc cgatcaggat gcccggtctc
    19261 acgtgctctt cggctccgag ggctacgatc acgtgccgat cagcaaggcc gtgcaggctt
    19321 cgctcagcgt caatcccgcc ttcgcttcgg tgcctatcga cggccgctac tacgaagacg
    19381 gcgccatcac gcgcacctcc aacttcggcg aggcgatcgg ccgcaacgca acgctcgtgg
    19441 tcaccttgga cccgttcgtg ccctacgtct cgaagctacc gggcctcgcg catcgtcgcg
    19501 gggttctcta caacatcgat caggatatcc gaacgatctc gttcacccgc ttcgagcgcg
    19561 cgcgcaacgc cgttctccgc aagcatcccg aggtcagctc gtacacgttt ctgccgtcca
    19621 acaccttgcg gcgcctgctc tccaccaacc ccatggacca tcggccgtat ctcgagatct
    19681 ggcgcggtgc ctacctgtcc gcgctgcaac ggatcgatca gctctgtcac cgacttcggg
    19741 gcgatctggc ggaacaaggg atgctgctcg acaccggaat ggcggagcac gtggcggaac
    19801 aattgcggac cacgacccat ctctgcttcg ccgacttctt tccggatcgt caggtcgaga
    19861 tccagaagcg tccgttcatg cagccctctc aactcacatc ttcaccgccg cccgccgccg
    19921 acgcttcaga ctcgcttggt tccgttcccc cttctcacgg cgagtgagag ccctatgctc
    19981 ccctgaagcg ataaggagtg atcatgagcc tagccgatca gaactgtgtc ccttgcaaag
    20041 gaggcgttcc tccgatgaaa caagagcaag ccgaggagct gctgcgcgag ctcgatgatg
    20101 gctgggttgt gaacgacaaa gggcatctcg agcgcacgta tgggttctcg aatttcgtcg
    20161 atgcgatggc gttcgcgaac cgcgtcggcg agatcgcaga ggcgcagaac cac
//
LOCUS       DASRZF010000030        12214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4494982, whole genome shotgun sequence.
ACCESSION   DASRZF010000030 DASRZF010000000
VERSION     DASRZF010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 12214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12214
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4494982"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(270..1142)
                     /locus_tag="VFG22_01440"
     CDS             complement(270..1142)
                     /locus_tag="VFG22_01440"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET6414929.1"
                     /translation="MDLNALELFVAAAEAGSFSAAARRTRVPLPTLSRRVRKLEDDLG
                     VRLLERGPHGLALTPAGTQLLAEATPALATLSQAEQRLYDSSGVAGTLRVSIPPHFEP
                     MQPVFSEFCRRYPAVRLDVFVTDRRVDLTADGIDVVVRVGEGGYSSYVGRTLARYRHR
                     VVGAPSLVQGVEIESPADLLSLACACWRTGGPPSWTMGDVELSLNPVLLTNDYAHLLH
                     LAVSGQAITELPPFMARDPLDDGRLVEILPDYPMPLQTIRALVVDTRAVSPLVRQFLD
                     FAAKALPGALGGLV"
     gene            1267..2358
                     /locus_tag="VFG22_01445"
     CDS             1267..2358
                     /locus_tag="VFG22_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6414930.1"
                     /translation="MTSKPQIRMLTGIFLIASVTISCASTQHRGANGNEATFDSSAGV
                     ETAAPAPTAAKTHVPVLEEVRADAIPVDPDIGVATIALEGGTYVVTDGIWQSAFVVTS
                     RGVIVLDAPESFGTKLRDAIAAVTDQPIKVLVYSHAHKDHIGGSANFADIPRLEIVAL
                     DSVRRFLEEKQDPNRLVPNSTFSTAKTIRLGGKTVALEQHGNYHADEGDLFIYVPQSK
                     FLMAVDTLAPGYVPFMNLDLTSNFHEYLKVFDDILPYDFDVFIGGHLTQLGDRADVEL
                     TQQYVRDVYETTKRVHDSTDMMAVMARTAQAEGGWDNKYLLFQAFLDQVTAEAAAEVQ
                     ARWIDRLAGVDVFVDSHVRTALIYVRWDD"
     gene            2367..3338
                     /locus_tag="VFG22_01450"
     CDS             2367..3338
                     /locus_tag="VFG22_01450"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HET6414931.1"
                     /translation="MRAWILRQPGGPEAFELTDIPRPEPRPGQVLIEIREFGLNRSEW
                     FTRIGESPTVRLPRVLGIECVGTVVRAPGGELEEGQRVAAMMGGMGREFDGSYAEYTS
                     VPAANVFPLETELPWDVVGALPEMLQTCHGSLHTGLGVEPGETLLIRGGTSSIGLTTL
                     AMAKAAGLRVISTSRSDAKVDVLRKAGADEVVIDDGKLGPTIRRDHPGGVDRVLELIG
                     TTTLLDSLQCVRPGGVVCMTGILGGEWELASFRPMGHIPTGVRLTSYSGGASDISRED
                     LQRYVSMVERGDLTIRTGPVWSFDQLPLAHQAMDENRANGKMVVVVE"
     gene            3295..3753
                     /locus_tag="VFG22_01455"
     CDS             3295..3753
                     /locus_tag="VFG22_01455"
                     /inference="COORDINATES: protein motif:HMM:NF014997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HET6414932.1"
                     /translation="MRTARTARWWSWSSSAGGGNGLDRAGTYTPIIVIGLEGWQKTVS
                     LWSIWILAVAGVVFSVFVRESPRWVRFGLYIAMGWFSLFIIPALYKTSGSEPVALMVC
                     GGVLYSVGAIVYATKRPDPWPDTFGFHEIFHVFVIAGCACFAATIWIWLL"
     gene            complement(3863..4567)
                     /locus_tag="VFG22_01460"
     CDS             complement(3863..4567)
                     /locus_tag="VFG22_01460"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6414933.1"
                     /translation="MSKPPKNALIVGVGPGLGGAAAKRLAADGYRVAVAARDVDKLSD
                     IATETDGLAVAMDATDPSSIEAGIARVEEEFGEIHTVVWNVGGGGFGDLEKVGIEALE
                     LSFDTNTRGLFVLAKQLVPAMAARGDGALIITGATASLRGKPFTTAFAAGKAAQRSLA
                     QSLARQYGKQGVHVALIIIDGMVDLPSTRERMRDTPQEAFVSPEGFADAVSFLLHQRR
                     GAWTFELDIRPHVENW"
     gene            complement(4612..5058)
                     /locus_tag="VFG22_01465"
     CDS             complement(4612..5058)
                     /locus_tag="VFG22_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414934.1"
                     /translation="MNTTQINALRVTAVLWVLWGIVHAFFGVAIMSMDASEGFAAIAA
                     GVAPGAVSADYHPAVGAILNQHAWNLLWFGVVTTLGGVMVWRRNMTAIWVSAMVGGLA
                     DLGYLVFVDFGGFATFVPGTLMTLIAGSAILLGGWVWFAQHKKTEG"
     gene            5244..6116
                     /locus_tag="VFG22_01470"
     CDS             5244..6116
                     /locus_tag="VFG22_01470"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET6414935.1"
                     /translation="MEHWNELRTALMVARHGTVSAAAEALGIHRATVNRHIDLLEENF
                     GAPLFQRHARGYALTETGQQMLDVASRAEEMFNDLAGRSRARAGKLSGKLIVTALSGF
                     SPLVMPTIREFQIAHPDVELEFVAGAQLARLEYGEAHIAFRPGPRPETPDYVVGTFGQ
                     IRYGLYASRDYIARKGMPVPPRFDGHLFVGSLGHPSPLPYARWMNEHVSQGQLALLTR
                     NQTVQGVAIADGLGLGFLPEFEAIRNSDLVAVMPPTLEWSATLWLVTHADLHRTEKVQ
                     EFLRWVRKHNVLPT"
     gene            6250..8583
                     /locus_tag="VFG22_01475"
     CDS             6250..8583
                     /locus_tag="VFG22_01475"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HET6414936.1"
                     /translation="MVKRACSPFLVVALAMFLTSVGCRASSTPGSSPGLVVSCPAGGT
                     AIIETSRWRGEVTQPDFALRVVRPSGEALLETSGPLRFTTVTGQESPLETGVVTPWTE
                     TGTVTSISCDATSLDVELSAKAGEATLMMLSLMGDADPRALRITAQVDPAVSEGINRV
                     AIDLVHTSNDRYYGMGQRYEEAEHSGNELVVWTEEGCNFECVDELPTGFPIPFYLSSR
                     TYGLLIDDTRFSRFDFGKTDPAKTAIELSHDRLELRVFVGDDPLDVIEAYTHFAGRIP
                     ELPLPWVFAPWISANTKHRGDSPNAESRTREIAALMRSERIPTSGIWNEDWAGGDGEL
                     LYTADFHLDETHYPNWAGMIDDMHALGFRVFAYFQPYLTVGTDDYLYAADRDYLLRDP
                     DGAPATFFLVGNRSQLDLTNPDAVRWWQDTIFDRAARFGVDGWMNDFGEYVGATTVSA
                     DGRLGWEVHNDSPRLWARTARELWEARRPDGDWVFFSRSGYTGSWQYSPVVWTGDQTT
                     DWSEGDGLPTVIPAVQSLGISGVPVVATDIGGLLCLTTPPSDRELFYRWTQVGAMLPV
                     MREHNGQFACESNWLIDDDADSIEHWKTYATLHTALFPYLYTLVAQARDHGWPVVRHP
                     VLHFPDAPGSKERQDDQFMMGDRILVAPVIAEGARSRVVYFPPGDWVHWQTGETYTGP
                     AEIEVAAPLRVAPYFYRSGRIIPRFDTVIDTLVEEFDPDLAGFADANSSMTIDFFGEG
                     RDELSLWDGTTIACDASARTCEVTRAPLDRTYTFRFH"
     gene            complement(8793..9632)
                     /locus_tag="VFG22_01480"
     CDS             complement(8793..9632)
                     /locus_tag="VFG22_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HET6414937.1"
                     /translation="MPNLPLHKKKTLSAFRKSALGTWNTPYDPTVYGVLSLKMDEALR
                     YVDAFRKQTGKHLTVSHMVAKAVGAVLEEVPDANALLRFNQIYLRDSIGVFFQVVMEN
                     PKTGEIDLSGATIQKPNEKTLEQIVDEFSAKVSGVRRGTDKDLKASRDLMSRIPSWGV
                     GPMLRLTSFLSYTLNLDMSWAGMPKDLFGSVMITNVGSLGLPYALVPLVPYSRVPLLL
                     ALGSIEERAVVEDGQLAVRRVMDVGATFDHRILDGGHAAKMHRILRDWMEDPFGHFDT
                     LAA"
     gene            9871..10515
                     /locus_tag="VFG22_01485"
     CDS             9871..10515
                     /locus_tag="VFG22_01485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HET6414938.1"
                     /translation="MMVARGKKPTARGWSHAIAAVIALVFSIAMARKTSANVEHMLSV
                     IVFGASMVLLFSMSAVYHLGRYSDGVRLTLRAIDHSNIYLLIAGTYTPIIVIGLEGWQ
                     KTVSLWLIWILAVAGVVFSVFVRESPRWVRFGLYIAMGWFSLFIIPALYKTSGSEPVA
                     LMVCGGVLYSVGAIVYATKRPDPWPDTFGFHEIFHAFVIAGCACFAATIWIWLL"
     gene            complement(10707..11927)
                     /locus_tag="VFG22_01490"
     CDS             complement(10707..11927)
                     /locus_tag="VFG22_01490"
                     /inference="COORDINATES: protein motif:HMM:TIGR03901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HET6414939.1"
                     /translation="MLGSAVVLATIAVFVFVPATGIGQAPGDPERGTLDGSPLDDLPP
                     YISLLTDFGSRPDWSPDGERVLFIDGAPLGDVVELDVATGTMRRLTDGFEHMGFTRAH
                     YLPSGDLLLCGPTSGPRPSDDSPEAGRFTGVMSVLRTPFDDAPQALGMPCWEGIVTSD
                     VSPRIAWNRSDIDYTDPDLLSRVRDGISEIWTGELRYEDERVLLENIELVIDRTAVSP
                     FAVLEAQDFRRGTEELIFTAYAYAGGEVMGVDLSTGLVTNYSQSPVYEEAEGVAPNGA
                     WVLVERDLDNVFLPGPLDIWLLPLDGSQAWDRLTRFNRYRGWYASNPTVNPSGTHIAF
                     QLSIDGDVVAEGGGILLFDLAASGRSIAPPDSESMAGGGSGCSVSESPSDGAAWLIAA
                     LVMLRVRRRNGSVS"
BASE COUNT         2259 a         3844 c         3818 g         2293 t
ORIGIN      
        1 accccggtcc actccatcga gggctgtcga cggacttcat ccttgttttc gcctgatcac
       61 actgcggtcg cgacgccgac gtcctgagtg tggtcgcggc gcgcgctagc tcaggcgatg
      121 gtgtgtgctg agagctcgaa aggaccctgc gtgccaagct gcccagagtc tcgagggtgg
      181 gcatgtctcc cacatcggcc cttccctgcg ttatcccggc cccgattttt ccacatggca
      241 aaaacggttt tcgattccca ccgcctccac taaacgagcc cacccaacgc accagggagg
      301 gccttcgccg cgaagtcgag gaactggcga acgaggggag atacagcccg cgtgtcgacg
      361 acgagggcac gaatggtctg aagcggcatc ggatagtcgg gcaagatctc gacaagccgc
      421 ccgtcgtcga gcggatcacg agccatgaag ggtggcagct cggtgatcgc ttgaccggag
      481 acggcaaggt gcaggaggtg cgcgtagtcg ttggtgagga gcacagggtt caaactgagc
      541 tccacatccc ccatggtcca cgaaggcgga ccgcccgtcc gccagcacgc gcatgccagg
      601 ctgagaagat ctgctggact ttcgatctcg acgccttgca cgagcgacgg tgcgccgaca
      661 acgcggtgcc tgtagcgggc aagcgtccga ccgacgtagg aggagtagcc accttcacca
      721 acccgaacca ccacgtcgat tccgtcggcg gtgaggtcga cgcgccgatc ggtcacgaac
      781 acgtcgaggc ggaccgctgg gtatcggcgg cagaactccg agaagacggg ctgcatcggc
      841 tcgaagtgcg gcgggatcga cacccggagc gtgcctgcaa caccggacga gtcatagagg
      901 cgttgctcgg cctgtgacag cgtcgcgagc gcgggcgtcg cttcggcaag cagctgcgta
      961 ccggctggcg tcagggccaa cccgtgtggc ccgcgctcca gcagccggac tccgaggtcg
     1021 tcctcgagct tgcgtacccg acggctcagc gtcggcaacg gaacgcgcgt gcgacgggcg
     1081 gctgcggaaa aactcccagc ctccgcggca gcgacaaaga gctcgagtgc attgaggtcc
     1141 atcgtttccg ggccatcctc tcgtttttga gaggataggt ctcatatcga agtctatccg
     1201 ttcggaatcg gtaggtctat gttcaggcga gcccgagccg attgctcggc gccaaaggag
     1261 aatcccatga cctcgaagcc tcagatccgg atgcttaccg gcatcttcct tatcgccagc
     1321 gtcacgataa gctgcgccag cactcagcac cgaggagcca acgggaacga agccaccttt
     1381 gattcgtccg ccggggtcga gaccgccgcg ccggctccaa ccgcggccaa gacccatgtg
     1441 ccggtgctcg aggaagtcag agcagacgcg attcccgtgg atccagacat cggggttgcg
     1501 acgatagcgc tcgaaggtgg gacttacgtg gtgaccgatg ggatctggca atccgcgttc
     1561 gtcgtcacca gtcgtggcgt gatcgtactc gacgcgccgg agtcgtttgg gacgaagttg
     1621 cgcgacgcga tcgcagcggt aaccgatcag ccgatcaaag ttctggtgta ttcacacgcc
     1681 cacaaggacc acattggggg ttcggccaac ttcgcggaca ttccccggct cgagatcgtg
     1741 gcgctcgact ccgtccggcg cttcctcgaa gagaagcagg atccgaatcg tctcgtcccg
     1801 aacagtacct tctcgacagc caagacgatt cgcctcggcg gaaagaccgt cgcgctcgag
     1861 cagcatggca actaccacgc cgacgagggc gatctcttca tctacgtgcc ccagtccaag
     1921 tttctaatgg ccgtcgacac gcttgcgcct ggctatgtcc cgttcatgaa tctcgacctc
     1981 acgtcgaact tccacgagta cctcaaggtg ttcgatgaca ttcttccgta cgatttcgac
     2041 gtgttcatcg gtgggcatct cacccagctc ggggaccgtg ctgacgtcga gctcacgcaa
     2101 caatacgtgc gcgacgtgta cgagacgacg aaacgagtcc acgattcgac agacatgatg
     2161 gccgtgatgg cacggacagc gcaggccgag ggcgggtggg acaataaata cctcttgttc
     2221 caggcctttc tcgaccaagt caccgcggaa gccgcggcgg aggtgcaggc ccgttggatc
     2281 gacaggcttg ccggggtgga cgtgtttgtc gatagtcacg ttcgcacagc gctgatctac
     2341 gtgcgctggg acgattagga atgccaatgc gagcctggat actgcgacaa cccggaggac
     2401 ccgaagcctt cgagctcacc gacatcccgc gtccggagcc acggcccggt caggtgctca
     2461 tcgagattcg ggagttcgga ctcaatagat cggagtggtt tacgcgtatt ggcgagtccc
     2521 cgaccgtccg cttgccgcgg gtgctcggca tcgagtgtgt cggcacggtc gtccgcgcgc
     2581 ccggcggcga gctcgaagaa ggtcagcgcg tcgcggcgat gatgggcggc atgggccgcg
     2641 agttcgatgg ctcgtacgcc gaatacacgt cggtgcccgc cgcgaatgtg ttcccgctgg
     2701 aaaccgagct gccgtgggac gtggtaggcg ccctacccga gatgctgcag acgtgccacg
     2761 ggtcgctgca caccgggctc ggtgtggagc ccggtgagac gcttctcatt cgaggcggca
     2821 catcgtcgat tgggctcacc acgctcgcca tggcgaaggc cgccggtctc cgcgtcatct
     2881 cgacgtccag gagcgatgca aaggtagacg tgctccggaa ggccggcgcg gacgaggtcg
     2941 tgatcgatga cggcaagctc ggtcccacga ttcgccgcga ccaccctggc ggggtcgacc
     3001 gagtactgga gctcatcggg accacgacgc tgctcgactc gctacagtgc gtcagaccag
     3061 gcggtgtcgt ctgcatgacc ggcatcctcg gcggcgagtg ggagctcgcg tcgtttcggc
     3121 cgatggggca catccctacc ggcgtgcggc tgacctccta ttcgggcggc gcgagcgaca
     3181 tctcgagaga ggacctccag cgctacgtgt caatggtcga acgcggggac cttacgatcc
     3241 gcacgggccc cgtgtggagc ttcgatcaac tcccgctcgc tcaccaggcg atggatgaga
     3301 accgcgcgaa cggcaagatg gtggtcgtgg tcgagtagcg cggggggagg caacggcctc
     3361 gatcgcgctg ggacgtacac gccgatcatc gtgatcggcc ttgaaggatg gcagaagacc
     3421 gtaagtcttt ggtcaatctg gatcctcgcg gtcgcgggcg ttgtcttctc ggtcttcgta
     3481 cgagagtcac cgcgttgggt tcgcttcggc ctttacatcg cgatgggttg gttctcgctc
     3541 ttcatcattc cagccctgta caaaacgagc ggctcagagc cggtcgccct catggtctgc
     3601 ggcggcgtgc tctactccgt aggcgcgatc gtctacgcaa ccaaaagacc cgatccctgg
     3661 cccgacacat tcggcttcca cgaaatcttc cacgtgtttg tcatcgcagg ttgcgcgtgt
     3721 tttgcagcga cgatttggat ctggctcctt tagcccactg gtaccattcc ggtgccgcgg
     3781 cgactccgag cgtcccgcgg gatcatgcga ggaggggccc tcccgcagag gatcgagtct
     3841 gtggacgcgg atccgtcttg acctaccagt tctcaacgtg agggcggatg tcgagctcga
     3901 atgtccatgc gccgcggcgc tgatgcagga ggaaagatac ggcgtcggcg aagccctcgg
     3961 gcgagacgaa cgcttcctgc ggcgtatcgc gcatgcgctc gcgagtgctc ggcaggtcga
     4021 ccatgccatc gatgatgatc aacgccacat gaacgccctg tttgccgtac tggcgcgcga
     4081 gtgactgcgc gaggctccgc tgggccgcct tgccggcggc gaaggccgtg gtgaagggct
     4141 tgccccggag cgacgcggtc gcgcccgtaa tgatcagcgc accatcgccg cgggcggcca
     4201 tggcaggaac cagctgcttt gcgagaacga agagaccgcg ggtgttggtg tcgaaggaga
     4261 gctcgagggc ctcgatgccg accttctcca agtcaccgaa ccccccgccg ccgacgttcc
     4321 acaccaccgt atgaatctcg ccgaactctt cttcgactcg agcaattcca gcctcaatgg
     4381 aggagggatc ggttgcatcc atcgcgacgg cgagcccgtc agtctccgtc gcgatatctg
     4441 acagcttgtc gacgtcgcga gccgcgacgg caacccgata accatcggcc gcaagtctct
     4501 tggctgcggc gcctcccagt ccgggaccaa ccccgacgat caatgcattc ttcggcggct
     4561 tggacatggc tcgaaacctc ctagctctgg gcgtgggccg tagcggccgc gttatccctc
     4621 agtcttcttg tgctgcgcga accagaccca accgccaagc agaatggcgg aaccggcgat
     4681 cagggtcatg agcgtgcccg ggacgaacgt cgcaaagccc ccgaagtcca cgaacacgag
     4741 atagcccagg tccgccaggc cgccaaccat ggcgctgacc cagatcgcag tcatgttgcg
     4801 ccgccagacc atcaccccgc cgagcgtcgt gacgacgcca aaccacaaca agttccacgc
     4861 gtgctggttc aaaatggcgc cgacagccgg atgatagtcc gcacttacgg ccccgggagc
     4921 aacgccggca gcgattgcgg caaagccttc cgaggcgtcc atcgacatga tcgccacgcc
     4981 gaagaaggcg tggacgattc cccagaggac ccaaagaacg gcggtgactc gcagggcgtt
     5041 gatttgtgtg gtgttcatcg cagcgcctca cgtggcgtga tctgattgca agagactggt
     5101 ggcgctttct acgtttccac gcggttcgat gcgccgtgct tcatctgcct gtcaaaatag
     5161 gctgcgcgat ggttgacgac aacttgcctt tttgcgcatg gtgtgcgcgc tggtacagca
     5221 tgtcttcagt caggtgagcg agcatggaac attggaacga gcttcgaacc gctctcatgg
     5281 tcgcgcgtca tggcacggtc agcgccgctg ccgaggcgct tgggatccat cgggcgacgg
     5341 tgaacagaca catcgatctc ctggaggaga acttcggtgc accgcttttc cagcgtcacg
     5401 cgcgcgggta tgcgctgacg gaaacaggcc aacagatgct ggacgtcgct tcacgcgcag
     5461 aggagatgtt caacgatctc gcggggcgca gtcgcgctcg cgctggcaag ctgtcgggga
     5521 agctcatcgt gacagcgctc tcagggttct cgcccctggt catgcccacc atacgggagt
     5581 tccagatcgc tcatcccgat gtcgaacttg agtttgtcgc tggcgcacag ctcgcgcgct
     5641 tggagtacgg cgaggcccac atcgcgtttc gtccgggtcc aagaccggaa accccggact
     5701 atgtcgtggg gacgttcggg cagattcggt atggcctcta tgccagccga gactacatcg
     5761 cgcgcaaggg catgcccgtc ccgccccgtt tcgatggcca tctgtttgtg gggtcgctcg
     5821 ggcacccgag ccctttgcca tatgcacgtt ggatgaacga acacgtatct cagggacagc
     5881 tagcgcttct cacgcgtaac cagaccgtcc agggcgtggc catcgcagac ggactggggc
     5941 tcggattcct tcccgagttc gaagcgatac gcaacagcga cctggtggcg gtcatgcccc
     6001 ccaccctaga gtggtcggcc acgctctggc tcgtcactca tgccgatctc caccgcactg
     6061 agaaggtgca ggaattcttg cgttgggtcc gcaagcacaa cgtcctgcca acctaggccg
     6121 cgttggtcgg gaccaaggtc tcctttcgag gcggaacgct cgccgagcgg atagcgagcc
     6181 agctgagttg gccggcgagc tcggtctcat ccccggacac accgccgatc gatgctatct
     6241 tgccgctcga tggtgaaacg cgcttgttcc ccgtttctcg tcgtcgcgct ggcgatgttt
     6301 ctcacatcgg tgggttgcag agcttcgtcg acacctggtt cttcgccggg ccttgtcgtg
     6361 tcctgcccgg cgggcgggac agcgatcatc gagacatcgc gttggcgagg agaggtcacg
     6421 caacccgact tcgcgcttcg cgtcgtgcgg ccgagtggtg aagcattgct cgaaacctcg
     6481 ggaccgctcc gtttcacgac ggtcaccggg caggagagcc ccttagaaac gggggtggtc
     6541 accccttgga cagagacggg gaccgtcacg agcatcagct gcgatgcgac ctccctggat
     6601 gtcgagctga gcgccaaagc aggcgaggcc acgctgatga tgctctccct catgggcgac
     6661 gctgaccccc gtgccttgcg cattaccgca caagtcgatc ccgcagtgag cgagggcatc
     6721 aaccgcgtgg cgatcgacct cgtgcacacg agtaacgatc gctattacgg aatgggtcaa
     6781 cgctacgaag aggccgaaca cagtggcaac gagctcgtcg tgtggactga ggaagggtgc
     6841 aacttcgagt gcgtggatga gctgccgacg ggttttccca tccctttcta cctgagctcg
     6901 cgcacgtacg gcttgctgat cgacgatact cgattcagtc gcttcgattt cggcaagacg
     6961 gatcccgcga agacggcgat cgagctttct cacgaccgcc tcgagctacg cgtcttcgtc
     7021 ggcgatgacc cactcgatgt catcgaagca tacacacact tcgccggacg gatccccgag
     7081 cttccgctgc cctgggtgtt cgcgccgtgg atatccgcga acacgaaaca ccgcggcgac
     7141 agccccaacg ccgagagccg gacgcgagag attgcagcgc tcatgcgcag cgagcgcatt
     7201 cccacgagtg ggatctggaa cgaggactgg gccggaggcg acggggagct tctctacaca
     7261 gcggacttcc acctcgacga aacccactac ccgaactggg ccggcatgat cgacgacatg
     7321 cacgcgctcg gattccgcgt gtttgcatac ttccagccgt atctgaccgt tggcacggat
     7381 gactatctgt atgccgcgga ccgcgactac ttgcttcgcg atccggatgg agctccagcg
     7441 accttcttcc tcgtggggaa ccgttctcag ctcgacctga cgaacccgga cgcggtccgt
     7501 tggtggcagg acacgatctt cgaccgcgcg gcacggttcg gcgtggacgg ttggatgaac
     7561 gatttcggcg agtacgtcgg agcgacgacg gtttctgcgg acggccgact cggctgggag
     7621 gtgcacaacg actctcctag gctttgggcg cgcacggccc gcgagctctg ggaggcgcgg
     7681 cgccccgacg gtgactgggt attcttctcg cgctccggct acacgggctc ttggcagtac
     7741 tcaccggtgg tctggaccgg cgaccagacg acggactggt cagaggggga cggcctgccc
     7801 acggtgatcc ccgccgtcca gtcactcggc atcagcggtg tacccgtcgt cgccacggac
     7861 atcggcggac tcctgtgcct cactacacca cccagcgatc gcgagctgtt ctaccggtgg
     7921 acccaagtgg gggcgatgct gccggtcatg cgcgagcaca acgggcagtt cgcctgcgaa
     7981 tccaattggc tcatcgacga cgatgccgac agcatcgagc attggaagac ctacgccaca
     8041 ctgcacacgg cgctctttcc gtacctctac acgctggtcg cgcaggcgcg cgatcacgga
     8101 tggcccgtcg tgcggcatcc cgtgcttcac tttccagacg ctcccggctc caaagagcgt
     8161 caggacgacc agttcatgat gggcgatcgg atcctcgtcg cgccggtcat cgcggagggc
     8221 gcgcgcagcc gtgtcgtcta tttcccgcca ggcgattggg ttcactggca gaccggtgaa
     8281 acgtacacgg gaccggccga gatcgaggtg gctgcgccgc ttcgagtcgc tccgtacttc
     8341 tatcgcagtg gccgaatcat tccccgattc gacacggtca tcgacacgct ggtcgaggaa
     8401 ttcgaccccg acttggctgg cttcgccgac gcgaattcat ctatgaccat cgacttcttt
     8461 ggagaaggcc gagacgagct ctcactctgg gacggcacga ccatcgcctg cgatgcgtca
     8521 gcgcgcacat gcgaggtcac cagggcgccc ctcgaccgga catacacctt tcgcttccac
     8581 tgaatgccga gtgtgcctga aacgaacggt ccatacgggc ttggccatcg tcggctcgtt
     8641 tcggcaccag ttcgccgatt ttgtgaacgt tcgaccggag cggtggacgg ccttgggtac
     8701 ctcgcccaag tccagccttc gctactgcaa cttcaatggc cgcgccgccg tcgagaacat
     8761 ctcgcgagta tcctcgcctc cgggcccagg cgctaggccg ccagcgtgtc gaaatgtccg
     8821 aatgggtctt ccatccagtc tcggagaatt cgatgcatct tggcagcgtg ccctccgtcg
     8881 agaatccgat ggtcgaatgt cgcgccaacg tccatcactc ttcgcacggc cagttgaccg
     8941 tcctcgacga ccgctcgttc ttcaatcgac ccaagcgcga gcaacagagg cacacggcta
     9001 tacggaacca gcggcactag tgcatacgga agccccaagg agccgacgtt cgtgatcatg
     9061 accgagccga agaggtcctt cggcatcccg gcccagctca tgtcgagatt cagcgtgtag
     9121 ctcagaaagc tcgtgagtcg gagcatgggg cccacgcccc aggaagggat ccgcgacatg
     9181 aggtcgcgcg aggccttcaa gtccttgtcg gtgccccggc gaacaccgct cactttggcc
     9241 gagaactcgt cgacgatctg ctcgagcgtc ttttcgtttg gtttctggat ggtcgcgccg
     9301 ctcaggtcga tttcgccggt ctttgggttc tccatgacga cctgaaagaa gacgccaatg
     9361 ctgtcgcgga gatagatttg attgaagcga agcagtgcgt tcgcatccgg aacctcttcg
     9421 agcacggcac cgacggcctt ggcgaccatg tgactcacgg tgagatgttt cccggtctgt
     9481 ttccggaacg catcgacgta tcgcagagcc tcgtccattt tgagactcaa aactccatac
     9541 acggttgggt cgtacggggt attccaagtc ccgagagcgc tcttgcgaaa cgcggatagc
     9601 gtcttcttct tgtggagcgg aagattcggc atggcggaag catacgccat cgcgcaaacg
     9661 aggaggagga ggaggatcgt tcacgaatcc tgtgcctcct cagaaccccg tcgatggcgc
     9721 aatcgttggt gtttccgtgc tcaacgtttt gaggaacgac agcaccgacg gcgagtctct
     9781 tgggatcgaa cctcacaatg acgggaaaag tttagtcggc gtgttcacgg ctcgtcgctg
     9841 tgactccgtg acctaagcgt gccagggacg atgatggtcg cacgagggaa aaagccgacg
     9901 gctcgtggtt ggtcgcacgc catcgcggcg gtgattgcgc tcgtcttcag catcgcgatg
     9961 gcaaggaaga cgtcggcgaa tgttgagcac atgctcagcg tcatcgtttt tggcgccagc
    10021 atggttctgc ttttttcgat gagtgcggtt taccatttag gccgttactc agacggcgtg
    10081 cgcctaacgc ttcgcgccat cgaccactca aacatttatc ttctcatcgc ggggacgtac
    10141 acgccgatca tcgtgatcgg ccttgaagga tggcagaaga ccgtaagtct ttggttaatc
    10201 tggatcctcg cggtcgcggg cgttgtcttc tcggtcttcg tacgagagtc accgcgttgg
    10261 gttcgattcg gcctttacat cgcgatgggt tggttctcgc tcttcatcat tccagccctg
    10321 tacaaaacga gcggctcaga accggtcgcc ctcatggtct gcggcggcgt gctctactcc
    10381 gtaggcgcga tcgtctacgc aaccaaaaga cccgatccct ggcctgacac attcggcttc
    10441 cacgaaatct tccacgcgtt tgtcatcgca ggttgcgcgt gctttgcagc gacgatttgg
    10501 atctggctcc tttagccaac tggtaccatt tcggaagcgc aattgcgccg tactagctcg
    10561 ttgtgtcggc ttgcggtgca ggctgaacct cgctcgaatc gtctgcgcgc atttctgctc
    10621 cgtctccccg catctcacaa cttcctttct cagttggagc ggccgcgctc cggcccgtcg
    10681 aatagagtgg gtcggcgcgg caccgtctac gacacggagc cattccgacg ccgcacgcgc
    10741 aacatcacga gagccgcgat gagccaagcg gcgccgtcgg acggactctc gctcaccgag
    10801 catcccgagc cgccgccggc catggactcg gaatcgggcg gggcgatcga acgaccgctc
    10861 gccgcgaggt cgaagaggag aatgccgccg ccctcggcca ccacgtcccc atcgatcgaa
    10921 agctggaagg caatgtgcgt cccgctgggg ttcactgtcg gattcgacgc gtaccagcct
    10981 cgatagcggt tgaagcgcgt gagccgatcc caggcctggg agccgtcgag cggcaggagc
    11041 caaatgtcga gcgggcctgg taagaacacg ttgtcgagat cgcgctcgac gaggacccag
    11101 gcgccattcg gggcaacgcc ctccgcctct tcgtagacag ggctctgcga gtagttcgtc
    11161 accagcccgg tcgagaggtc gacgcccatg acctcaccgc ccgcgtacgc gtacgcggtg
    11221 aagatcagct cctcggtgcc tcggcggaag tcctgcgcct cgagcactgc gaacggcgag
    11281 acggccgtgc ggtcgatcac cagctcgatg ttctcgagca ggacgcgttc gtcttcgtag
    11341 cggagctccc cggtccagat ctccgagatg ccgtcccgga cccggctgag caggtcgggg
    11401 tcggtgtagt cgatgtcaga gcggttccac gcaatgcggg gcgacacgtc cgacgtcacg
    11461 attccctccc agcacggcat gccgagcgct tgcggagcat cgtcgaatgg cgtgcgcagg
    11521 acagacatga cgccggtgaa gcgacccgct tcgggactgt cgtcactcgg ccttgggccc
    11581 gaggtcgggc cgcagagcag caggtcgccg ctcggcaggt agtgggctcg cgtgaagccc
    11641 atgtgctcga agccgtcagt cagtctccgc atcgtccccg tcgcgacatc gagctcgacg
    11701 acatcgccga gaggcgctcc gtcgatgaag agaacccgct cgccgtcggg cgaccagtca
    11761 ggccgagagc cgaagtcggt cagcaggctg atgtacggcg gcaggtcgtc gaggggggag
    11821 ccgtcgagcg tccctcgttc ggggtcgccg ggagcctgac cgatccccgt tgcggggacg
    11881 aagacgaaca cagcgatggt tgcgagcact acagccgagc ccaacatgtg gccagagcct
    11941 accacaactc ccatcgaggc tcgtgacgtg cgcgaatacg acatgaagcg gcgtttgttc
    12001 ttgctacgct ctcccgacta cccgagaaac agccgtcccg atgtttggcc caatgagaaa
    12061 gctgatccca cgcaccctag tgtttgcatt cgcgtgtgcg gtgctcggcc catggggctg
    12121 cggcgacagt tcggagcaga gcgcgccggc gattgagcat ccgtttcgtc cacccacagg
    12181 gtcgatctac cttcttgcag ggcaggactc ggag
//
LOCUS       DASRZF010000031         4733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2087936, whole genome shotgun sequence.
ACCESSION   DASRZF010000031 DASRZF010000000
VERSION     DASRZF010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4733
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2087936"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="VFG22_01495"
     CDS             complement(<1..433)
                     /locus_tag="VFG22_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414940.1"
                     /translation="MKRAAIVIIIVAGLGFLGGFLYGSLTTNVIPTITEQRAAGPNRM
                     VPESWREVRLSPGHSQHVINERLECDDCHDPAESGFELPDTGVCTQCHEDQASLAHVN
                     LDGSPMDCYTCHIFGSQPEVFGRWHCTRCHGPFETEGLAGLS"
     gene            complement(430..1488)
                     /locus_tag="VFG22_01500"
     CDS             complement(430..1488)
                     /locus_tag="VFG22_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414941.1"
                     /translation="MSKTGSIIALCALLSTALGCAKMLGLEQEDEDHAFEHRAHLVEG
                     INCLKCHAGIPEAGETGPLHFPSTADCVECHQEPHNTNTCSDCHGRARNRQAAEMARK
                     HLRFSHAVHQEEGAGDCVRCHVNVTTSSDYVRVKHPTCFSCHAHKGQWDTRDCGGCHV
                     DLPGEMTRPESHVIHDGNFLREHGVEANAQGDLCTACHTQRQCAACHGITVPAIPSRL
                     NFDEPMRNTMHRANFISVHDQQAVASPAQCLICHSELFCVDCHTRLGISGQNPERGSP
                     HPPGWSGPPGTSNTHGRAARLDPLSCASCHGGAGEMLCVGCHIVGGPGGNPHPPGFNS
                     RLDTANDLPCRLCHIGGR"
     gene            complement(1485..2813)
                     /locus_tag="VFG22_01505"
     CDS             complement(1485..2813)
                     /locus_tag="VFG22_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414942.1"
                     /translation="MHGVWGAPILEPSEPSDHDARWGRNASRKSRRARRWALGLAASI
                     ALLLLAPLPRAAAQPHSVGVELWGQLDPNVGLVILEGSTRKYPYIDAEALVWAGAGSP
                     QFGLSGNEATFEALVVAIKVRDPRGITDTRLGRFILSTGAVRAVHVDGGHITLRAPSK
                     THFEAFGGVPVDGNIEGRKYDWIAGGRISQGLGKIGTLGGSYYNRYNAGQRSDEEVGV
                     DFALRAAQWLDMAAKFSWEIVNPGLAEVLATISSQTKNHKLRGEAFATKRNPTRFLQQ
                     TSLFTVLGDLGTFKTGGNLFWRAAPRLDLTVEGAALEAEDDWGWGGYLRGVLRTDDEG
                     KGSVLGELRRQDVATSQWTGIRLAAVIPLFEDHNQTVGLMPEFEIIIPDARTQETGNA
                     WPWGRLALQWRHEKGWALAAAAEASSNQAQSIDVRGLARVSYQHKWQGKQ"
     gene            2808..3485
                     /locus_tag="VFG22_01510"
     CDS             2808..3485
                     /locus_tag="VFG22_01510"
                     /inference="COORDINATES: protein motif:HMM:NF015237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HET6414943.1"
                     /translation="MHVSPLSWIALTCALVAAGLMIYYLIARPPLNARVKLLMMLGVG
                     VLPIMTAMIGNMEGLHATEQQAFCGSCHTMDMHIADANDPDSNSLAAIHSRTIKFGGQ
                     SCYVCHKDYGMFGYAMTKLGGMGHVYMYLSEFMWYDMEEALPKVHIARPFKNDNCMQC
                     HSTTGAIWTGVADHNGLLEDVRAGKTSCASAGCHGYAHPFSKPDGETPSQGEAHSALN
                     LPSPEGT"
     gene            3482..3757
                     /locus_tag="VFG22_01515"
     CDS             3482..3757
                     /locus_tag="VFG22_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414944.1"
                     /translation="MNPKEKIEKFLDSASRSRLMVYACVLGLIGLALMSWSLFEQGWI
                     PVMMAMSVGQGIGTLSFALFLVVVLIDLRQAEFRISEPPPRHTPKGD"
     gene            complement(3754..>4733)
                     /locus_tag="VFG22_01520"
     CDS             complement(3754..>4733)
                     /locus_tag="VFG22_01520"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET6414945.1"
                     /translation="VSVAFGGYMSGLVPGLPSWVTVTGILLLLAVINFRGMRESSTTN
                     IVATMVELTGLLIVIVAGALFLVERPDPEIPRTIAISSNVGWRAIASGAALSFFAFIG
                     FEDMVNVAEEVKDPERNMPRAILTALGVTGVVYLLVVLVATSVVPPESLAASKAPLLA
                     VVQRSTDIIPDRLFTLIALFAVANTGLLNFIMGSRLIYGMARQGLLPSALGRVHATRR
                     TPHLAIITVLLVALALALSGTLTYLAGTTSLLLLLVFFTLHLALIAIKRRDARRPRTF
                     CAPHLVPFVGALTCLALMPFVPRGSLVTAAMILALGSGIVWLRARRSSG"
BASE COUNT          883 a         1467 c         1529 g          854 t
ORIGIN      
        1 tggatagtcc tgcgagcccc tccgtctcga aaggcccatg gcaacgcgtg caatgccatc
       61 gtccgaacac ctcgggctgc gagccgaaaa tgtggcaggt gtaacaatcc atcggactgc
      121 catcgaggtt gacgtgcgcc agcgatgctt ggtcctcgtg gcattgggta cacacgccgg
      181 tatcgggcag ctcgaagccc gattccgccg ggtcgtggca gtcatcgcac tcgaggcgct
      241 cgttgatcac gtgctgcgag tgccccggag aaagccgaac ttctcgccag ctttccggca
      301 ccatgcggtt gggcccggcg gctcgctgct ctgtgatggt cggaatcacg ttggtggtga
      361 gactcccgta aaggaaccca ccgagaaaac cgagccccgc aacgatgatg attactatcg
      421 cggcacgctt catcgtccac ctatgtgaca tagccggcag ggaaggtcgt tggccgtatc
      481 gaggcggcta ttgaaacccg gcggatgtgg gttgccgccc ggcccgccca cgatgtggca
      541 gcccacgcaa agcatctcgc cggcgccccc atggcagctt gcgcaggaca agggatcgag
      601 ccgtgcggcg cggccgtgcg tgttgctggt gcctgggggg cccgaccatc caggcgggtg
      661 gggactcccg cgctcggggt tctgtccgga aataccgagg cgagtgtgac agtcgacgca
      721 gaagagctcg ctgtggcaga tgagacactg cgccgggctc gcgacggcct gctgatcgtg
      781 cacggagatg aagttggccc tgtgcatcgt gttgcgcatc ggttcgtcga agttcaatcg
      841 agacgggatc gccggcaccg tgatgccgtg gcaagcggcg cactgacgtt gcgtgtggca
      901 ggccgtgcag aggtcgcctt gcgcgttggc ctctacgcca tgctcacgca ggaagttccc
      961 gtcgtgaatc acgtggcttt cgggccgcgt catctctccg ggcagatcca cgtggcagcc
     1021 gccgcaatcc cgggtgtccc attggccctt gtgcgcgtgg cagctgaaac aagtcggatg
     1081 cttcactcga acgtagtcgg agctcgtggt gacgttcacg tgacatcgca cgcagtcacc
     1141 ggcgccctct tcctggtgga ctgcgtgcga gaaacggaga tgcttgcgcg ccatctcggc
     1201 cgcctgacga tttcgggcgc gcccatgaca gtccgagcag gtattggtat tgtgcggttc
     1261 ctggtggcac tcgacgcagt cggcggtcga tggaaagtgg agtggacccg tctcacccgc
     1321 ctctgggatc ccggcgtggc acttgagaca gttgattcct tcgacgaggt gtgcgcggtg
     1381 ctcaaacgcg tggtcctcat cctcctgctc gagaccgagc attttggcgc agcccagagc
     1441 cgtgctcagg agcgcgcaga gtgcgatgat ggaccccgtc ttgctcattg cttcccctgc
     1501 cacttgtgct gataggagac acgcgcaagc cctcgcacgt cgatgctctg cgcctggttg
     1561 gagctcgcct cggcggctgc tgccagtgcc cagccttttt cgtgccgcca ttgaagggcg
     1621 agacgccccc agggccatgc gttgcctgtt tcctgggtgc gagcgtctgg gatgatgatc
     1681 tcgaactccg gcatgagacc gaccgtctgg ttgtggtcct cgaagagggg gatgaccgcg
     1741 gcgaggcgaa tgcccgtcca ctgagaggtg gcgacgtcct ggcgccgcag ctcgccgagc
     1801 acgctgccct ttccttcgtc gtccgttctc agtacgccgc gcaaataccc gccccagccc
     1861 cagtcgtcct ccgcctcgag cgcagcgccc tccaccgtga ggtcgagcct cggagcggcc
     1921 cgccagaaga ggttcccgcc cgtcttgaag gtgccgaggt caccgagcac ggtgaacagc
     1981 gatgtttgct gcaggaagcg agtcggattg cgcttcgtcg caaaggcctc gccgcggagc
     2041 ttgtggttct tggtttggga cgagatggtc gccagcactt cggccagacc cgggttcacg
     2101 atctcccagg agaacttggc ggccatgtcg agccattgag cggccctgag ggcgaaatcc
     2161 acgccgacct cttcgtcgga acgctgcccg gcgttgtagc ggttgtaata cgagccaccg
     2221 agggtgccga tcttcccgag gccctgggag atgcggccgc cagcgatcca gtcgtacttt
     2281 cgaccctcga tgttcccgtc gaccggcacc cctccgaatg cctcgaaatg ggtcttcgaa
     2341 ggggcgcgga gcgtgatgtg accgccgtcg acgtggaccg cgcgcaccgc gcccgtggaa
     2401 agaatgaagc gacccagtcg cgtgtccgtg atccccctgg gatcccgcac cttgatggcg
     2461 actaccagcg cttcgaaggt ggcttcgttg cccgacagac cgaactgtgg cgagccggcg
     2521 ccggcccaaa ccagagcctc tgcgtcgata tagggatact tgcgtgtcga gccctcgagg
     2581 atgacgagtc cgacgtttgg gtccagttgc ccccagagct cgactccaac gctgtgcggc
     2641 tgagcggcgg cccttggcaa aggcgcaagg agaagcagcg cgatcgacgc cgcgaggccg
     2701 agcgcccagc ggcgcgcgcg ccggctcttc cggcttgcat tacgacccca tcgtgcatca
     2761 tggtcagatg gctccgacgg ctctagaatc ggggcgcccc aaacaccatg cacgtatccc
     2821 cgctgtcctg gatcgctctg acctgtgctc tcgtcgcggc cgggctcatg atctactact
     2881 tgatcgcccg tccgccgctc aacgcgaggg tgaagcttct gatgatgctc ggcgtcggcg
     2941 tccttccgat catgaccgcc atgatcggaa acatggaagg gctccatgcc accgagcaac
     3001 aagcgttttg cggaagctgt cacaccatgg acatgcacat cgcggatgcc aatgacccgg
     3061 acagcaattc cctcgccgcg attcactccc gcaccatcaa gttcggaggc cagagttgct
     3121 acgtctgtca caaagactac ggcatgttcg gctacgcgat gaccaagctc ggtggcatgg
     3181 ggcacgtgta catgtatctc agcgagttca tgtggtacga catggaagag gcgctcccca
     3241 aggtgcacat cgcccgtccc ttcaagaatg acaactgcat gcagtgtcac agcacgaccg
     3301 gggcgatctg gacaggggtt gcggaccata acggacttct cgaagacgta cgtgccggga
     3361 aaacgagctg cgcgagcgcc ggctgccacg gctatgccca tccctttagc aaaccagacg
     3421 gagaaactcc atcccaggga gaggcccatt cggccctaaa tctcccttct ccggagggaa
     3481 cgtgaatccc aaagagaaga tcgagaagtt tctggacagc gcatcgcgtt cgcgcttgat
     3541 ggtctacgct tgcgtccttg gtttgatcgg cctcgctctc atgagctggt cgctcttcga
     3601 gcaaggctgg attccggtga tgatggcaat gtccgtcggc caaggcatag gtacgttatc
     3661 attcgcgctg ttcctcgtgg tagtgcttat cgacctcagg caagcggagt tcaggatttc
     3721 ggagcctccc ccgcgacaca cgcccaaagg cgactagccg ctcgaacgcc gggcgcgcag
     3781 ccagacgatg cccgaaccga gtgcgaggat catcgcggcg gtcacgagcg agccacgcgg
     3841 cacgaagggc atcagtgcga ggcacgtgag tgcgccgacg aacggaacca ggtgtggcgc
     3901 gcaaaatgtg cgcggacgcc gggcgtcgcg gcgcttgatc gcgatgagcg cgagatgcag
     3961 cgtgaagaac accaggagca gcaagaggct cgtcgtacct gcgagatacg tgagcgtgcc
     4021 ggacaacgcc agggcgagag ccaccaacaa caccgtgatg atcgccaggt gaggggtgcg
     4081 gcgcgtggca tgcactctgc cgagcgcgga tggaaggagg ccctggcgcg ccatgccgta
     4141 gatcaagcgc gagcccatga tgaagttcag cagaccggtg ttggcgacgg caaagagcgc
     4201 gattaacgtg aacaagcggt ctggaatgat gtcggtggac cgctgcacga ccgcaagaag
     4261 cggggctttg gacgctgcca gggactcggg cggaacgacg ctcgtcgcga cgaggacgac
     4321 gagcaggtac acgaccccag tcacgccgag ggcagtcagg atggcgcggg gcatgttgcg
     4381 ctctgggtcc ttcacctctt cggccacgtt caccatgtct tcgaagccga tgaaggcgaa
     4441 gaagctcagt gccgcgccac ttgcgatggc gcgccagccg acgttgctgc ttatggcgat
     4501 ggttcgggga atctcaggat cgggtcgttc tacgagaaag agcgctccag ccacgatcac
     4561 gatcagcaat ccggtgagct cgaccatcgt ggcgacgatg ttcgtcgtgg aggactcgcg
     4621 catgccccgg aagttgatca cagcgagcag aagcaaaata cccgtcaccg tgacccagga
     4681 cggcagccca ggaacgagcc cgctcatgta gcctccgaaa gcgactgata cag
//
LOCUS       DASRZF010000032         3272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152 k141_22333,
            whole genome shotgun sequence.
ACCESSION   DASRZF010000032 DASRZF010000000
VERSION     DASRZF010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22333"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="VFG22_01525"
     CDS             <1..924
                     /locus_tag="VFG22_01525"
                     /inference="COORDINATES: protein motif:HMM:NF027968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase C-terminal domain-containing
                     protein"
                     /protein_id="HET6414946.1"
                     /translation="NEVIRYVHPDECQLSYNPLLMALLWEALATQDVRLLEHSMRTRF
                     RLPAGCAWVNYLRCHDDIGWTFDDDDARSIGIDPASHRRFLNQFYTGEFPGSYARGKK
                     FQENALTGDVRVSGTLSSLAGLEEALERDDSELIERAVRRITLLMGIQVSIGGLPLLY
                     AGDEYGMINDYTYLHDPRKVNDSRWVHRCRKRWEAKEDLVDLETIEWRFFRETSKLFN
                     LRKRLPALCDGGMEVIETQNPRIFAYLRAHAEQRVLVVTNFSETPERLDPRFLAPWLP
                     GGEALDHLTGNRLSVSDGLVLEHHRAVWLEV"
     gene            complement(940..1941)
                     /locus_tag="VFG22_01530"
     CDS             complement(940..1941)
                     /locus_tag="VFG22_01530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET6414947.1"
                     /translation="MTTSTRAVTVALSVDLVIGTAKVVTFLLTGSTAIMAEMLHSVAD
                     LTNQGLLVIGIARSRRGPDSNYPYGFGRSRYIWALLSAAGVLFVGSGVSVVRGVERVW
                     APEPLEHLGWGLGVLLVSLVAESISFLFGLSAVRSSAREAKQSVRQYIGMGPDPMGVA
                     VVLEDASAVFGVLLAAAGIALAEFTGNPAWDGAASVAIGLLLGASAIFLINRNRRLLL
                     GPAPSSESVARMMSILEGSPVVSKIQDVKVSQLGADAVRFKAEVTFDGRALAKRLLKG
                     TDLDATWSSLTGPEALERLLVEFGSEVTDAIGDEIDRLEAELAEAAPEARHVDLEPD"
     gene            1987..2199
                     /locus_tag="VFG22_01535"
     CDS             1987..2199
                     /locus_tag="VFG22_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414948.1"
                     /translation="MSAPSSMSASDAEDLVLRPHRASQSWAQTTLDDRAELLRSLACV
                     QRVGTHERQDGVRGRPMTMPVDDEKG"
     gene            2207..2542
                     /locus_tag="VFG22_01540"
     CDS             2207..2542
                     /locus_tag="VFG22_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414949.1"
                     /translation="MFVGHYGPSLGLRRASEGVPLWALFLAVQFVDVLWALLVIAGVE
                     KVRITPGFTASSPLDLYYMPYTHSLAASIAWALLLGWFASFVWSRRGGFAVGACVLSH
                     WFDYAGVTL"
     gene            complement(2521..>3272)
                     /locus_tag="VFG22_01545"
     CDS             complement(2521..>3272)
                     /locus_tag="VFG22_01545"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HET6414950.1"
                     /translation="PGEPLIAVFVDFENLAIGAQVTESGRFQIELVLKRLLEKGRIVY
                     KRAYCDWSRYHNAVREFHGQGIGLVDIPQTRMSGKNSADIHMVVDALDLCYSKDHIDT
                     FALLSGDSDFSPLVYKLKENDKRVIGVGVKKSTSDLLIAGCDEFIYYDDLIRAAKPKP
                     ARSRGPKKAKSKKDEAVDIFMETIESLDQDYDTLWGSMIKQTLRRLYPGFNESYYGYR
                     NFPDLMKDVERRGLIELEYDEERGNYKVTPA"
BASE COUNT          627 a         1028 c          978 g          639 t
ORIGIN      
        1 aacgaggtga ttcgttacgt ccacccggac gagtgccagc tttcttacaa tcctctgctc
       61 atggcactcc tgtgggaggc gctcgcaacc caggacgtga ggctcctcga gcactcgatg
      121 cgcacccgct ttcgtcttcc ggcgggttgc gcctgggtca actacttgcg ctgtcacgac
      181 gacatcggat ggaccttcga cgacgacgac gcccgcagca tagggatcga cccggcaagc
      241 catcggcgct ttctgaacca gttctacacc ggcgagtttc cagggtcgta tgcgcgtggg
      301 aagaagttcc aggagaatgc gctcaccggc gatgtgcgcg tctcagggac actttcgtca
      361 ctcgcagggc tcgaggaggc gctcgagcga gacgatagcg agctgatcga gcgagccgtt
      421 cgtcgcatca cgctgctgat gggcattcag gtgagcatcg gtggacttcc tctgctctat
      481 gcgggtgatg agtacggcat gatcaacgac tacacctacc ttcacgaccc gcggaaagtg
      541 aatgacagcc gctgggtgca tcgctgccgc aagcgctggg aggctaagga agatctggtg
      601 gacctcgaaa cgatcgagtg gcgcttcttc cgtgagacat cgaagctctt caatctccgc
      661 aagaggcttc ccgcgctctg cgacggcggc atggaggtca tcgagacgca gaacccacgg
      721 atcttcgcgt acctgcgcgc ccacgcggag cagcgagtcc tggtcgtgac caatttctcg
      781 gagacgcccg aacgtctcga cccgcgcttt ctcgcgccct ggctccccgg tggcgaggcg
      841 ctggaccatt tgacggggaa tcgtctctca gtctccgatg ggctcgtgct cgagcatcat
      901 cgcgccgtct ggctcgaagt ctgagcgcgg cgggcgcgtt cagtctggct cgagatccac
      961 gtgccgagcc tccggtgcgg cctccgcaag ctcggcctcg agccgatcga tctcatcgcc
     1021 gatcgcatcg gtgacctcgc taccgaactc caccagaagt cgctcgagcg cctccggccc
     1081 agtcagggaa gaccaggtcg catctagatc ggttcctttc agcagccgct tggcaagcgc
     1141 gcgaccatcg aaggtgacct cagccttgaa acggaccgcg tccgctccca gctgtgaaac
     1201 cttcacatct tggattttcg aaacgaccgg actgccttcg agtatggaca tcattcgagc
     1261 cactgactcc gacgagggcg cgggaccgag caggagtctc cgatttcgat tgatgagaaa
     1321 gatcgcggac gcaccgagga gcagtccgat ggcgacggag gcagcaccat cccatgcggg
     1381 attcccagtg aattcggcca aggcgattcc cgccgcggcg agcagaacgc cgaacacggc
     1441 tgacgcatct tcgagcacca ccgcaacccc catcggatcg ggtcccattc cgatgtactg
     1501 ccgcacggac tgcttcgcct ctcgggccga gctccgaacc gcggacaaac caaagaggaa
     1561 cgatatgctc tccgccacca gggacacgag caggacaccc aagccccagc cgaggtgctc
     1621 cagcggttcg ggcgcccaga ctcgctcgac tccgcgaacg accgacacgc cgctcccaac
     1681 gaagagcact ccggctgccg atagaagcgc ccaaatgtag cgcgatcgac caaatccgta
     1741 cggatagttg ctatctggcc cacgtcgcga gcgcgcgatc ccaatcacca agaggccctg
     1801 attcgtgagg tccgccacgg agtggagcat ttccgccatg atcgccgtcg atccggtcag
     1861 caggaaggtc acgaccttgg cggtaccgat cacgagatcg accgatagcg caaccgtcac
     1921 agcgcgcgtg ctcgtcgtca cccgagtact atagcgtcga tccctgatat agtcgcccgt
     1981 aagaagatga gcgcaccatc ctcgatgagc gcgtccgacg cagaggatct cgtcctgagg
     2041 cctcatcgag catcccagag ttgggcacag acgacgttgg acgaccgcgc ggagctcctt
     2101 cgaagcctcg cgtgcgttca acgtgttgga acgcacgaac gccaagacgg tgtacgtggc
     2161 cgcccaatga cgatgccggt tgacgacgag aaggggtagg tgggcaatgt tcgttggaca
     2221 ctatgggccg agcttgggcc ttcgccgcgc ttccgaggga gtgcctcttt gggcgctttt
     2281 tcttgccgtc cagttcgtcg acgtgctctg ggcgctgctt gtcatcgctg gggtcgagaa
     2341 ggtgcggatc acgcctgggt tcacagcatc gagcccactg gacctctact acatgcccta
     2401 tactcacagc ctcgcggcct cgatcgcctg ggcactgctg ctcggttggt tcgcgagctt
     2461 cgtctggagc cgtcgtggcg gattcgctgt cggagcatgc gtgctgtcgc actggttcga
     2521 ctacgccggc gtgaccttgt agttgccgcg ctcctcgtcg tactcgagct cgatcaggcc
     2581 tcggcgttcg acgtccttca tcaagtcggg gaagttgcga tacccgtagt agctctcgtt
     2641 gaaccctggg tagagacgtc gcagggtttg cttgatcatc gagccccaga gcgtgtcgta
     2701 atcctggtcc aacgactcga tggtttccat gaagatgtcg accgcctcgt ccttcttgct
     2761 cttggctttc tttggaccac gactgcgcgc tggcttcggc ttggctgcgc gaatcaaatc
     2821 gtcgtagtag atgaactcgt cgcaacccgc gatcagaagg tcggaagtgg acttcttgac
     2881 cccgactccg atgacgcgct tgtcattctc cttgagcttg tacacgagtg gcgagaagtc
     2941 gctgtctccg gaaagaagcg cgaacgtgtc gatgtggtcc ttcgagtaac agagatccag
     3001 ggcatcgacc accatgtgga tgtccgcact gttcttgccg ctcatgcggg tctggggaat
     3061 gtcgaccaga ccaatccctt gaccgtggaa ctcccgcacc gcgttgtgat agcggctcca
     3121 atcgcagtag gcgcgcttgt aaacgatgcg gcccttttcg agcaagcgct tcagtacgag
     3181 ctcgatttgg aaacggcccg actccgtgac ctgggcgcca atggccaagt tctcgaaatc
     3241 gacgaagaca gcgatcagcg gctcaccagg ca
//
LOCUS       DASRZF010000033         8995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3808043, whole genome shotgun sequence.
ACCESSION   DASRZF010000033 DASRZF010000000
VERSION     DASRZF010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 8995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8995
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3808043"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..954)
                     /locus_tag="VFG22_01550"
     CDS             complement(160..954)
                     /locus_tag="VFG22_01550"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6414951.1"
                     /translation="MGKRFENKVVWITGGGSGIGRALALAFANEGATVAVSGRRDDRL
                     QEVVQELESHGGKGFAVRCDVTDEASVADAAQKVARKLGGIDVAVANAGFSVAGRIEK
                     LSAADWRRQLDVNVIGVAMTARYALPHLRERKGRLALIGSVASMLSTPGVGAYSASKY
                     AVRAIGQALAAELHGSGVSCTTIHPGYIESEIAQVDNQGRFDASREDRRPKNLMWPAD
                     RAARVMVDAIAKRKREYTFTGHGKLGAFAGRHAPGLVHFGLTRGKK"
     gene            complement(1006..4164)
                     /locus_tag="VFG22_01555"
     CDS             complement(1006..4164)
                     /locus_tag="VFG22_01555"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET6414952.1"
                     /translation="MSIPAFSVRQPVLVNLVAISTVVVGALAMSVMHRESLPTMPTGW
                     GNVTTTYVGASPEEIEQLITIPIENAIGDVDNVEEIWGVSKEGVSYVSFHFDADVEDV
                     TSAMMEVSNEINRIDDLPLDAERPVVREVKVDFPTLVVAIRGEVPEVVLRKVGKDMAD
                     RIERIPGVSGTWRNGIRDREFRVDVDPDRLTAYQLSLTSLTDALRSRAANIPAGTTKG
                     DGDARLVRGMTRVGDVEELAEVVIRPDAEGGSIRVADVAEVKDTFAPGKYSGRVNGEP
                     AIVLTVRKEEQADSVLISEDVRALVERLRPTLPRGVTIDLFGDAATEVEHSLGTLYSN
                     AALGLLLVLGLLWMAIGARNATMAALGLPVALAGAIIAMQMMGITINMMSLLALILCL
                     GVVVDDAIIIIENIYRHMEEGMPRRKAAVLGTEEVFWPVVASTLTSWAAFLPLLMMEG
                     VLGDFFAIIPKVVVASLAASLVEAMFILPSHMSDFGSLSRRKTLEPGPPKTRLQRAGE
                     RILETYERLLRWSLTHRKTVVLGAYGLCALMVGAALELKDVILLTEGDPDMFDVRVHM
                     PTDASKEETEALLQELEARILALGNTDVEATLAVRGMTRTDMGFSSGDHMGMVSVFIV
                     PATRRSSVHGGRDLVTKVSGLYDDVVGPTRLEIVEVRPGPPRGAPVAVRIRGEEQERL
                     VELAEQVLAEVRVTPGTRNSNHDYELGKQELQIKVDEQRAALHGLTPEMVSRWLRSAF
                     GATPASTLREGDDEIDIVVQLREDVQQDPERIASLQLVAPNGDQVAIREIAEVSHGRG
                     PGVIRRYQQKRVVSVLSGIDEEVTNSREVNATLQKKLASLEAANPDIRFEYGGQFEET
                     EESVASLLRAFLVALLLIYTILATQFQSFSQPLVVMAAIPLSFIGVTGGFLLSSGAIG
                     LIALVGVVGLTGIVVNDSLVLVDFVNKRRALGTPLDEAIVESGKLRLRPIFLTSITTI
                     AGLFPLSLATESLPHLAPMAQVIVYGLTFSTLLTLLVVPCLYRLDVDLAARASKRFAP
                     LTRWVTDPGAASAEDPEESTAE"
     gene            complement(4161..5276)
                     /locus_tag="VFG22_01560"
     CDS             complement(4161..5276)
                     /locus_tag="VFG22_01560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET6414953.1"
                     /translation="MLPQPRALLLLIAMAGANACTETGPVTTEAPPPPVHAITIEAEA
                     VTPRSTATAEILAKRESQMRSETAGRVVEVRVEAGDRVDEGEILLRLDVGRPASAAQA
                     ANAAVAQADARLDQAERELARTRKLVKTGGLAEQKLDDAEDALRLASASKDAARAEAS
                     LARRGLTDAVVRAPFGGTIVERSVELGEYLAPGSPLLTLADTSLLKARVLLDPREAID
                     VALGSPAEVSVYARPGEAFKGKVVRVGDVIDPRTRRLPVEIEIDDERGRLRPGLVAKF
                     EVVTGPAKMEIRVPLDGVFERFGSQHVYVIEDGIAKRRVVELGSVRAGYAEIIDGVTE
                     GETVVVKGVTRVVDGSKVQVVPLESARASSGEQTGAP"
     gene            complement(5337..6176)
                     /locus_tag="VFG22_01565"
     CDS             complement(5337..6176)
                     /locus_tag="VFG22_01565"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HET6414954.1"
                     /translation="MVIDPESLGYETPFIEFNYTWRDAVIYALGVGASADEELDYLYE
                     GRGPKVLPTFSTIPTFAAFDALVDRIGCDRTGMVHHSQEVNLFKALRPDARLRVKGRV
                     AGLYDLKRMAISVFSIDAYDEDDDLIVRGEVTLLLRNDGGFGGARPPKTERVILPERA
                     PDFEIRDQVAPTQALLYRLSGDYNPLHADPEFAAEVGFPKPILHGLCTYGYAGRAVLR
                     GACGGDGGRLAMLRGQFSNPVFPGDVLVVRGWNEGQRVVLRVSTEERPETVCLSNAYA
                     VLS"
     gene            6247..7587
                     /locus_tag="VFG22_01570"
     CDS             6247..7587
                     /locus_tag="VFG22_01570"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HET6414955.1"
                     /translation="MSKTIKRVAIVFSGGPAPAANAVIAAATTSFLEAGIEVLGIFHG
                     YTNLQEYNPTTNPMLPSEHYRLLAPADIRGIRNTRGIIIGTARVNPGAAIEMPSDLLN
                     PEKTERLARIYRALADLKVDALISIGGDDTLKTANFLYHYQRHQVPDGHRVRVVHLPK
                     TIDNDYRGIDFTFGFFTAVDVMAKELLNLRADCIATSSYFVVETMGRKAGWLSYGVAI
                     AGEASMVLSTEDVEGDLAIEERRVDPITGEESVELRLSITALVDRIVDLMLEREKRDQ
                     FYGTIVLAEGLAELLPDHSLDGMPRDEHGHVRLSAFDLAKVVSDRVQTRYEERTGRRK
                     KVRHIQLGYESRCSPPHAFDVMLASQLGIGAFRALVEEELDGHMVSVSGQLDLHYVEF
                     SQLINPQTLRTEIRFIRTGSDFHRLARFLETQTGRFFAWSPGLRREPETKKPSG"
     gene            complement(7604..7831)
                     /gene="rpsU"
                     /locus_tag="VFG22_01575"
     CDS             complement(7604..7831)
                     /gene="rpsU"
                     /locus_tag="VFG22_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HET6414956.1"
                     /translation="MVESVQCKPIEVHVGERGLERAVKHLKRKMATEGILRELKRRRH
                     YMKPSIKKRKKAAEAARRRRKRVRQISERPF"
     gene            complement(7943..>8995)
                     /locus_tag="VFG22_01580"
     CDS             complement(7943..>8995)
                     /locus_tag="VFG22_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6414957.1"
                     /translation="EVQTSASFLKEEVTEEDIAEVVSKWTGIPVNKMLEGEKDKLLHM
                     EDRLRERVIGQDEAIVAVSNAVRRSRAGLSEPNRPIGSFLFLGPTGVGKTELARALAE
                     FLFDDDKAMIRLDMSEYMEKHAVSRLIGAPPGYVGYEEGGQLTEPVRRRPYSVVLFDE
                     VEKAHPDVWNVLLQVLDDGRLTDGQGRTVDYKNVVIILTSNVGSHHIMALDDDASMRK
                     AVMEDLRDAFRPEFLNRLDETIIFHRLDRAQLRNIVDVQLRNFEKRLAERDLDLEITD
                     AAKDFLANVGYDPAYGARPLKRAIQRYLENGLAEDILAGRFAAGDTVRVEAGEGKLIF
                     EGASRPEGHEPRVNAE"
BASE COUNT         1687 a         2916 c         2671 g         1721 t
ORIGIN      
        1 ggataccgca gggattcggg tcgccatctc aaccaaatca ccgccggcgc gggattcaac
       61 aagggcaagg ttggcgcgga agtggcgctc cgccaggaca tcggaggccg caaagagacg
      121 gacctcctct tcatgttccg catcgtggtc gagtagctgc tacttcttcc cgcgcgtgag
      181 cccgaagtga acgagcccgg gtgcgtgacg gcccgcgaac gcgccgagct taccatgccc
      241 cgtaaacgtg tactcacgct ttcgtttcgc gatggcgtca accatgaccc gcgctgcccg
      301 gtctgccggc cacatgagat tcttgggacg tcgatcttcc cgggacgcgt cgaatcggcc
      361 ttggttgtcc acttgggcga tctcgctctc gatgtagccg gggtgtatcg tcgtgcagct
      421 tacgccgctc ccgtgaagct ctgcggcgag ggcctgcccg atcgcccgga cggcatactt
      481 cgatgcggag taggctccaa cacccggtgt cgagagcatc gacgcaacac tgccgatcag
      541 tgcgagccgt cccttgcgtt cgcgaagatg agggagcgcg tagcgggccg tcatggcaac
      601 tccaatcacg ttgacgtcga gctgccgcct ccagtccgct gcagagagct tctcgatccg
      661 gcctgcgacc gagaaccctg cgttcgctac tgcgacgtcg atcccgccca gcttacgggc
      721 gactttctgc gccgcgtcgg caaccgatgc ttcatcggtc acgtcgcatc ggacggcgaa
      781 tcctttcccg ccgtgtgact cgagctcttg cacgacttct tgaagccgat catcgcgccg
      841 cccggagacc gctaccgtcg ccccttcgtt ggcaaaagca agcgcgagcg cgcggccgat
      901 gccgctgccc ccgccggtga tccagacgac cttgttttcg aaacgcttcc ccattttcag
      961 cgcgtagcag ctcgctgccc ggcctgccac ggcccggggc acccgctact ctgccgtcga
     1021 ctcttcgggg tcttcggccg aagcggcgcc tggatcggtg acccagcgcg tcaacggcgc
     1081 aaagcgcttc gaagcgcggg cggcgaggtc gacgtccaag cggtagaggc aaggcaccac
     1141 caataaggta agcagtgtcg agaacgtgag gccatagacg atgacctgcg ccatcggcgc
     1201 caggtgtggc agactttcag tcgcgagcga gagtgggaag agccctgcaa tcgtcgtgat
     1261 gctggtgagg aagatcggcc tcaagcgaag cttgccggac tcgacgatcg cctcatcgag
     1321 cggggtcccg agagctcggc gcttgttgac gaagtccaca agtacgagcg aatcgttcac
     1381 cacgatcccg gtcaggccca ccacgcccac caaggcgatc aagccgattg cccccgagct
     1441 caagagaaag ccacccgtca ctccaatgaa ggacaaggga atcgctgcca tcacgaccaa
     1501 aggctgcgaa aagctctgaa attgggtcgc cagaatcgtg tagatgagca acagcgccac
     1561 gagaaacgcg cgaagaaggg aggcgactga ttcctcggtc tcctcgaact gccccccgta
     1621 ctcaaatcga atgtccgggt ttgcagcctc caaggacgcg agcttctttt gcagcgtggc
     1681 gttcacctcc cggctgttcg tgacctcctc gtcgataccc gagagcacgc ttacgacccg
     1741 cttctgttgg tagcggcgga tcacgcccgg tcctcgtccg tgtgacacct cggcgatctc
     1801 tcgaatcgcc acctggtcgc cgttcggtgc gacgagctgc agcgaagcga tccgttccgg
     1861 gtcctgctgc acgtcttccc tcagctgaac gacgatgtcg atctcgtcgt cgccttcccg
     1921 cagagtcgag gctggcgtcg caccgaaagc gctgcgcaac catcggctta ccatctcggg
     1981 agtgagtcca tggagtgcgg cacgctgttc gtcgaccttg atctgcaact cctgcttgcc
     2041 gagctcgtag tcgtggttcg agttgcgcgt accgggggtc acacgcactt ccgcaagcac
     2101 ttgctcggca agctcgacga ggcgctcctg ttcttccccc cggatgcgaa cagcgaccgg
     2161 tgcgccgcgc gggggtccag gtctcacttc gacgatttcg agccgggtcg gcccaacgac
     2221 gtcgtcgtag agtccgctca ctttggtgac cagatcacga cctccatgca cgctgctgcg
     2281 tcgagtcgct ggcacgatga acacggagac catgcccatg tggtctcccg agctgaaacc
     2341 catgtccgtt ctcgtcatcc cgcggacggc aagcgtcgct tccacgtcgg tgttgccgag
     2401 cgccaggatt cgagcttcga gctcctgcag cagtgcctcg gtctcttcct tcgaggcgtc
     2461 ggtcggcatg tggacgcgaa cgtcgaacat gtccgggtcg ccttccgtga gtaggatgac
     2521 gtctttgagc tccaaggcgg ccccgaccat gagcgcgcag agtccgtaag cgcccaacac
     2581 tacggtctta cggtgcgtca gcgaccagcg taggaggcgt tcataagtct ccaaaatacg
     2641 ctcgcccgcg cgctgcaatc tggtcttcgg gggacccggt tccagggtct tccttcgcga
     2701 aaggctgccg aagtccgaca tgtgactcgg caagatgaac atcgcttcga cgagcgaggc
     2761 tgcgagcgag gcgacgacca ccttggggat gatcgcgaag aagtccccaa gaaccccttc
     2821 catcatgagc agcggaagaa acgcggccca gctggtcaac gtcgacgcaa cgacgggcca
     2881 gaaaacctcc tcggtgccga gcaccgccgc cttccgtcgc ggcatgcctt cttccatgtg
     2941 ccgatagatg ttctcgatga tgatgatcgc gtcgtccacg acgacgccca gacagaggat
     3001 caatgcgagc agggacatca tgttgatcgt gatgcccatc atttgcatcg cgatgatcgc
     3061 ccccgccaga gcgaccggaa gccccaaggc cgccatggtg gcgttgcgcg cgccgattgc
     3121 catccagagc agccccaaga cgagcaagag accgagagcc gcattcgaat agagcgtgcc
     3181 aagagagtgc tcgacctccg tcgctgcgtc gccaaacaga tcgatggtca cgcctctcgg
     3241 cagagttgga cgcagacgct cgacgagtgc gcgtacgtcc tcgctgatga gcacgctgtc
     3301 ggcctgctcc tcctttcgaa ccgtcagaac gattgccggc tctccgttga cccgaccgct
     3361 gtactttccg ggcgcgaagg tatccttcac ctcggccacg tctgcgaccc ggatcgaacc
     3421 gccctcggcg tcgggacgaa tcacgacctc ggccagctct tccacgtcgc cgactcgcgt
     3481 catcccccgc acgagccgag cgtcaccgtc gcccttcgtg gtgcccgcgg ggatattggc
     3541 tgcgcgtgaa cggagcgcgt cggtcaatga agtgagggag agctgatacg cggtcagtcg
     3601 gtcggggtcg acatcgactc gaaattcgcg gtcccgaatg ccatttcgcc aggttcctga
     3661 cacgccagga atccgctcga tgcggtccgc catgtctttg cctaccttgc ggagcacgac
     3721 ctcgggaacc tcgccacgaa tggcgaccac cagtgtcgga aagtctacct tcacctcgcg
     3781 gaccaccgga cgttccgcgt ccagcggcaa atcgtcgatt cgattgatct cgttcgagac
     3841 ttccatcatc gcgctcgtga cgtcctcgac gtcggcatcg aaatgaaagc tcacgtacga
     3901 aacgccttcc ttcgagacac cccagatctc ttcgacgttg tcgacgtcgc cgatggcgtt
     3961 ctcgatcgga atcgtgatca gttgctcgat ctcttcgggc gatgcgccga cgtaggtagt
     4021 cgtcacgttg ccccatcccg taggcatggt cggcagcgac tcgcggtgca tcacgctcat
     4081 cgcaagtgcg ccgaccacca ccgtcgaaat cgccacgagg ttgacgagca cgggttgccg
     4141 aacggaaaag gcagggatgc tcatggcgcg ccggtttgtt ccccggaaga ggctctggcg
     4201 gattcgagcg ggacaacctg cactttcgag ccgtcgacga ctcgggtcac gcccttgacg
     4261 accaccgtct cgccctctgt gacgccgtcg atgatctcgg catagcctgc tcggacggag
     4321 ccgagctcga ccacgcgccg cttcgcgatc ccgtcctcga tgacatagac gtgctggctc
     4381 ccaaaacgct cgaagacccc atccaaaggc acccggattt ccatcttcgc tggcccagtc
     4441 accacctcga acttcgcaac caagcccgga cgaagacgtc cgcgttcatc atcgatctcg
     4501 atctccactg ggaggcggcg ggttctcgga tcgatcacat cgcctacacg cacgactttc
     4561 cctttgaaag cttcgcccgg ccgcgcatac accgatacct ccgctggcga acccagcgct
     4621 acgtcgatcg cttcgcgcgg atcgaggaga acacgcgcct tgagaagact cgtgtccgcc
     4681 aaagtgagta gcgggcttcc cggtgccaag tattccccga gctccacgct tcgctcgacg
     4741 atcgttccgc cgaacggcgc gcgcacgacc gcatcggtca gtccgcgccg ggcgagactc
     4801 gcctcggcgc gcgcagcatc tttggaagcg gacgcgaggc gcagagcgtc ttcagcatcg
     4861 tcgagcttct gctccgccag ccctcccgtt ttgacgagct tcctggttcg ggcgagctcc
     4921 ctctcggcct ggtcgagtcg cgcgtcggcc tgggccacgg cggcgttcgc ggcttgcgct
     4981 gcagatgccg gacgccctac gtcgagtcga agcagaatct cgccctcgtc gacgcggtca
     5041 cccgcctcga cgcgaacctc caccacccgt cctgcggtct cggagcgcat ctgcgactct
     5101 cgcttggcga gaatctccgc ggttgcggtg gagcggggcg tgacggcttc cgcctcgatc
     5161 gtgatggcgt gaaccggagg gggcggggct tcggtcgtga caggccccgt ctcggtgcaa
     5221 gcgttggcgc cagccatggc gattaacagc aagagagctc ttggctgcgg aagcattacc
     5281 ggagaaccct gtgtgcggat tcgagcttgg gcaagacgaa accccccgcg gtcagatcag
     5341 ctcagcacgg cgtacgcgtt gctgaggcag acggtttcgg gccgctcttc cgtgctcacc
     5401 cgcagaacga cgcgctggcc ttcgttccag ccccgcacca cgaggacatc gcccggaaag
     5461 actggattgc tgaattgccc gcgaagcatc gcaagtctcc ccccatcgcc gccacaagcg
     5521 ccgcgaagga ccgcccgccc agcatatcca taggtacaga ggccatgcaa aatcggcttt
     5581 gggaaaccca cttccgccgc gaactccgga tcagcgtgga gcggattgta gtcgccgctc
     5641 agacggtaga ggagcgcctg cgtcggcgcg acctggtctc gtatctcgaa gtcaggcgca
     5701 cgttcgggca ggattacacg ttcggtcttg ggcggacgcg cgccgccgaa gccgccatcg
     5761 tttcgcagaa gcaaggtgac ctcaccacga acgatcaggt cgtcatcctc gtcgtaggcg
     5821 tcgatcgaga agaccgatat cgccattcgc ttgagatcgt atagaccggc cactcgtcct
     5881 ttgacccgga gacgggcgtc gggccgaaga gctttgaaga ggttaacctc ttgactgtgg
     5941 tgaaccatac cggtccggtc gcagccgatg cggtcgacca gcgcgtcgaa cgctgcaaac
     6001 gtagggatgg tggagaaggt aggaagaacc tttgggcccc gcccctcgta gagatagtcg
     6061 agctcttcgt cggccgaggc ccctacccct agcgcgtaga tcacggcgtc tcgccaggtg
     6121 tagttgaact cgatgaacgg cgtctcgtag ccgaggctct cggggtcgat gaccatatcg
     6181 atgagcccga gtagagcgcc tgctgcaagc tatggcaatc cggcctcgcg gagcctacag
     6241 ttcagcgtgt ccaaaaccat caaaagagtc gcgattgtct tctccggtgg acccgcgccc
     6301 gcggcaaatg cggtgatcgc cgcggcgacc acatccttct tggaagccgg aatcgaagtc
     6361 ctgggaatct tccacggcta caccaatctt caggagtaca atcccactac caacccaatg
     6421 ctcccctccg agcactaccg cttgctggct ccagccgaca ttcgcgggat tcgcaatacg
     6481 cgcgggatca tcatcggaac ggcgcgcgtc aatcctggcg ctgcgatcga aatgccttcg
     6541 gatctcctca atcctgagaa gaccgagcgc ctggccagaa tctaccgcgc gctagcggac
     6601 ttgaaagtcg atgcgctgat ctccatcggt ggcgacgaca cactcaaaac ggccaacttc
     6661 ctctaccact atcagcgaca ccaggttccc gatgggcatc gcgttcgtgt cgtccatcta
     6721 ccgaagacca tcgacaacga ttaccgcggc atcgatttca cgtttggatt cttcacggca
     6781 gtcgacgtca tggcgaagga gctgctgaac ctacgggccg actgcattgc gacgagcagc
     6841 tatttcgtag tcgagacgat gggccgcaag gcggggtggc tctcctatgg ggtcgccatc
     6901 gcaggcgaag cgagcatggt gctctccact gaagacgtgg agggagatct ggcgatcgaa
     6961 gaacggcggg tcgatccgat caccggtgag gaatcggtgg agctccggct ttcgatcact
     7021 gcgctcgtgg atcgcatcgt cgatctgatg ctcgagcgag aaaagcgaga tcagttctac
     7081 ggcaccatcg tcttggccga aggcctggct gagcttcttc cagatcattc attggatgga
     7141 atgcctcgtg acgagcacgg tcacgttcgg ctcagtgcgt ttgacctcgc aaaggtggtc
     7201 tccgaccgcg tgcaaacgag gtacgaggag cgcaccggaa ggcgaaagaa ggttcgccac
     7261 attcagctcg gctacgagtc gcgctgctca ccaccccacg cgttcgatgt gatgctcgcc
     7321 agtcaattgg gcatcggcgc ctttcgagca ctcgtcgaag aagagctcga tggtcacatg
     7381 gtcagcgtca gcggtcagct cgacctccac tacgtcgagt tctcgcaact gatcaatccc
     7441 cagacgctcc gtaccgagat tcggttcatc agaacgggca gcgattttca tcgcctcgcg
     7501 cggttcttgg agacgcagac cggaaggttc ttcgcgtgga gtccgggact tcgtcgggag
     7561 ccagaaacaa aaaagccttc gggttgaatc cgaaggcttc gtctcagaac gggcgctcgc
     7621 tgatttggcg aacgcgcttg cggcgacggc gtgccgcctc cgcggctttc ttccgcttct
     7681 tgatcgaggg cttcatgtaa tggcgccggc gcttgagctc tcgaaggatg ccttcggtgg
     7741 ccatcttgcg cttgaggtgc ttcaccgcac gttcgagtcc acgttcaccg acgtgaactt
     7801 cgattggctt gcattgtacg gattcgacca tgaacgcttt tgctccttct aagagcgccg
     7861 gaggcttaca cagtgtacca gggcatgcaa gccagcccgg tttttccagg gtgagcagtc
     7921 ctaacaactc ataattgcgg acttattcgg cgttcacgcg tggctcgtgc ccttccggac
     7981 gactggcccc ttcgaagatc agcttaccct ctcctgcttc cacgcgaacg gtgtcgcccg
     8041 ctgcgaatcg gcccgcgagg atgtcctcgg caaggccatt ctcgaggtag cgctggatcg
     8101 cgcgcttgag gggccgggct ccgtaagccg gatcgtaacc cacgttggcg aggaagtcct
     8161 tggccgcgtc ggtgatctcg aggtcgaggt cacgctccgc gagccgcttc tcgaagttcc
     8221 gcagctgcac gtccacgatg ttgcgtagct gcgcgcggtc gagtcggtgg aagatgatcg
     8281 tttcatcgag tcggttcagg aactcgggac ggaacgcgtc gcgcaggtct tccatgacgg
     8341 ctttgcgcat cgacgcgtca tcgtcgagag ccatgatgtg gtgcgaaccc acgttcgacg
     8401 tgagaatgat cacgacgttc ttataatcga ccgtgcgccc ctgaccgtcc gtcaatcgcc
     8461 cgtcgtccag cacttgcaaa agcacgttcc agacgtcggg gtgcgctttt tcgacctcgt
     8521 cgaaaagaac gaccgagtac ggtcgccgac gaacgggctc cgtgagctga ccgccttctt
     8581 cgtagcctac atagccagga ggcgcgccga tgagacggga caccgcgtgc ttctccatgt
     8641 actcgctcat gtcgagccga atcatcgcct tgtcgtcatc gaacaggaac tcggccagag
     8701 cccgggcgag ctccgtctta cccacacctg tcggcccaag gaaaaggaac gagccgatcg
     8761 gacgattggg ctcactcagc cctgctcgcg aacgccgcac ggcgttcgac acggccacga
     8821 tcgcttcgtc ctgaccgatc acgcgttcgc gcagccggtc ttccatgtga aggagcttgt
     8881 ccttttcgcc ttccagcatc ttgttgaccg gaatgccggt ccacttggaa accacctcgg
     8941 cgatgtcttc ctcggtgacc tcttccttca ggaaggaagc actggtctgc acctc
//
LOCUS       DASRZF010000034         3912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1056001, whole genome shotgun sequence.
ACCESSION   DASRZF010000034 DASRZF010000000
VERSION     DASRZF010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1056001"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="VFG22_01585"
     CDS             <1..634
                     /locus_tag="VFG22_01585"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET6414958.1"
                     /translation="APYLKGFGLWLVSTVGNLGLGLAMFFVAIVISGALLPHGKAAAR
                     FVRKFAFMVAGERGPALADLAASSVQSVTRGVLGVAMIQSLLSGLGMLLVDVPGAGLW
                     TLLILIIAVMQLPPMLVLVPVIVYVFSTSPTWIAIVFTIWAALIGLSDNVLKPLLMGR
                     GSSVPMLVLFVGSLGGFISSGILGLFVGAVVLSLGYTVFLAWVDDADAPP"
     gene            complement(637..1857)
                     /gene="serA"
                     /locus_tag="VFG22_01590"
     CDS             complement(637..1857)
                     /gene="serA"
                     /locus_tag="VFG22_01590"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020797315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HET6414959.1"
                     /translation="MAVNAKKSRILLLEGIHETAIDSFAAAGYDNVQRLSHSLTDSDL
                     HDAIADVDLIGIRSRTHLDAQALHAAKSLKAIGCFCIGTNQVDLDVAGRMGIPVFNAP
                     FSNTRSVAELAIADIILLMRGIPEKSARARRGEWAKTARNAYEVRGKCLGIVGYGRIG
                     SQLGILAEGLGMRVLYHDVEHKLPLGNATPATTLKELLAAADVLSLHVPETPATRNMI
                     DEQALRSMRPGSLLINAARGTVVDIDALASALASGHIGGAAIDVFPVEPKSNQDTFES
                     PLRQFDNVILTPHIGGATEEAQLNIAREVVAKLVKYMDNGSTVSAVNFPQVTIPDHEG
                     RRRILHIHRNEPGVMEQINRVFSSHGVNVAAQYLETRADVGYVVMDIELDDAGPLLEE
                     LEAIPATIRVRLLR"
     gene            1933..>3912
                     /locus_tag="VFG22_01595"
     CDS             1933..>3912
                     /locus_tag="VFG22_01595"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HET6414960.1"
                     /translation="MRYLVVSLLLLSCACTNDRQRDFSLGACQEYGVSTPVEVPKSCP
                     LEIEGDGETARVFAIGAVIRYEEMESYAAFCKAWDDVVRTEVLPCLSDDKPNLLVFPE
                     NATLAGAFIGSRGTAGRGETASIPAFFSLFNNYADPIRYYAQQFPEASANERLVLGIT
                     DTLHRAFQTFPAMAKQYGVYVAVSSDFAPAELSEDPALLETLGDPDLDSVSSVYVATE
                     GTGYNWGLYFGPDGEEIGRVAKSYLVPAEEDVLDLSHGPLTQMRPIDLSFARSGMVIS
                     KDAWMPGVLHRLDALGANLMLQPEAFSGWGVEEYEGDWLPDIVRQSAWAHTQRHGAFR
                     HTVTPCIKGNLFELPFDCQSHVTSASSPSDTPRSFIGQDPYLGLLAVEPWVIEDPGPP
                     ASLEERRATLRDRGKRMLPGSGDPLEDAYAGGVIAANVALPFDGRVPSTGDGEPGAFG
                     PSTLVAEPRSAATHQRFPSLASDGDSALIAWMEGAPGEESVRAFITDLASPAEEVELD
                     EAGDVVRRLPRVAVAAGRMAVVWEEESRDQTARVVVATRAGESWRQMAITEPGVGTAW
                     EPDVAIDPVTGNYHVAWLDLRNGGRPKPWVARSEDGQSWETRQVDPGNAIVDNPRGDA
                     AFPKISARDGSVFVAFSDFREFSWDVYLSASGDGGL"
BASE COUNT          687 a         1158 c         1269 g          798 t
ORIGIN      
        1 cgcaccctac ctcaaaggct tcggtctctg gcttgtgtcc actgtcggca acctcggact
       61 cggcctggcc atgttcttcg tcgccatcgt gatatctggt gcactccttc cgcatggcaa
      121 ggcagccgcg aggttcgtgc gcaagttcgc cttcatggtc gccggagagc gcggccccgc
      181 actcgcggac ctcgccgcat cgagtgtgca aagcgtgaca cgcggggttc tcggcgtggc
      241 catgattcag tccttgctct ctggcttggg tatgctcctg gtggacgtac ctggagcggg
      301 cctctggacg ctcttgatct tgatcatcgc cgtgatgcag ttgccgccga tgctggttct
      361 cgttccggtc atcgtctacg tgttctcgac gagcccgact tggatcgcca tcgtattcac
      421 catttgggcg gcgctgattg ggctgagcga caacgtgttg aagccgctgc tgatggggcg
      481 cggttcgtcg gtcccgatgc tcgtgctgtt cgtgggttcc ctcgggggat tcatctccag
      541 cgggattctc gggcttttcg tgggcgcagt cgtcctctcg ctcgggtata cggtcttctt
      601 ggcgtgggtc gatgacgccg acgcgccgcc ctaaactcag cgaagaagac gcacacgaat
      661 cgtggccgga atggcctcga gctcctcgag caacggtccc gcatcgtcga gctcgatgtc
      721 cattacgacg tagccgacat ccgcgcgtgt ctccagatac tgcgcggcca cgttgacgcc
      781 atggctcgag aacacgcgat tgatctgttc catgacaccg ggctcgttcc tgtgaatgtg
      841 caggatacgc cgcctacctt cgtggtcggg gatggtgacc tgagggaagt tcaccgcaga
      901 caccgtagag ccgttgtcca tgtacttcac gagctttgca acgacctccc gggcgatgtt
      961 gagctgggct tcttcggtgg cgccgccgat gtgaggcgtg agaatgacat tgtcgaattg
     1021 tctcagggga gactcgaacg tgtcttgatt ggatttcggc tccacgggga agacgtcgat
     1081 cgcagcgccg ccgatgtgac ctgaggcgag cgctgaggcc agcgcgtcga tatcgacgac
     1141 agtcccgcgt gcagcattga tgaggagcga ccctgggcgc atcgaacgca gagcttgctc
     1201 gtcgatcata ttgcgcgtgg ccggggtctc gggaacgtgc aggctgagaa cgtccgccgc
     1261 cgccaggagc tccttgaggg tggtggcggg cgttgcgttt ccgagtggga gcttgtgctc
     1321 gacgtcgtgg tagaggaccc gcatgccgag accttccgcc agaattccga gctgactccc
     1381 gatccggccg taaccgacga tgcctaggca ctttcctctc acttcatagg cattgcgggc
     1441 cgtcttcgcc cactcaccgc ggcgcgctcg cgcagacttc tcaggaatgc cccgcatcag
     1501 caggatgatg tccgcgatgg caagctccgc cacgctgcgg gtattggaga acggcgcgtt
     1561 gaaaaccgga atccccatgc ggcccgcaac atccaagtcg acctggttgg tgccaatgca
     1621 gaaacaaccg atcgccttca agctctttgc cgcatggagt gcctgcgcat ctagatgtgt
     1681 tcgggacctg atgccgatca agtcgacgtc ggcgattgca tcatggagat ccgagtccgt
     1741 gagcgagtgc gagagacgtt gtacgttgtc gtagcccgcc gcggcgaagc tatcgatcgc
     1801 ggtttcatga attccctcga ggagcaggat tcgagacttt ttcgcgttca cggccagagt
     1861 ctgtgtcgat accggaacca ccgccaaccg aaatctgcga gacaagtcgg gtcgccgcgc
     1921 taggattgct ggatgagata tctcgtggtc tcattgctcc ttctcagctg cgcctgcacc
     1981 aacgacagac agcgcgattt ttcgcttggg gcttgccaag aatacggtgt cagcaccccg
     2041 gtcgaagtgc ccaagagctg ccctctcgag atcgaggggg atggtgaaac cgcacgcgtg
     2101 ttcgccatcg gagcggtgat tcggtacgag gagatggaga gctacgccgc gttttgcaag
     2161 gcatgggatg acgtcgttcg gactgaagtg cttccctgcc tctcggacga caaacccaat
     2221 ctcctggtgt ttcccgagaa cgcgacgctt gctggcgcct tcattggctc gcgaggaaca
     2281 gctggacgcg gggaaaccgc atcgattccg gcctttttct ctctgttcaa caactacgcg
     2341 gatccgattc gctactacgc acagcagttt cccgaggcga gtgcgaacga acgtctggtg
     2401 ctggggatca cggacacctt gcatcgtgcc ttccaaacct ttccggcgat ggcaaagcag
     2461 tacggagtgt acgttgcggt gtcctcggat ttcgccccgg cggagcttag cgaagacccg
     2521 gcactcctcg aaacactcgg cgatccggat ctcgattcag tgtcgagcgt ctatgtggcg
     2581 accgagggca ccggctacaa ctggggtctg tactttggtc cggacggcga ggagatcgga
     2641 cgtgtcgcca agagctatct cgtgccggca gaagaagacg tgctcgatct gagtcacggg
     2701 ccactgaccc agatgcgccc gatcgatcta tcgttcgctc gctctgggat ggtgatttca
     2761 aaggacgcgt ggatgcccgg ggtcttgcat cggctcgacg cactcggcgc gaacctcatg
     2821 ctgcagcccg aagcgttttc gggctggggc gttgaagagt atgagggcga ttggctcccg
     2881 gacatcgtgc gacagtccgc ttgggctcac actcagcgac acggagcgtt tcgccatacg
     2941 gtcacgccct gcatcaaggg caacctgttc gagctaccat tcgactgcca gtcgcatgtg
     3001 acgagcgcca gcagcccatc cgatacgcct cgctcgttca tcggtcagga cccatacctc
     3061 ggactgctcg cggtcgagcc ctgggtgatc gaagatcccg gcccgccggc gtcgctcgaa
     3121 gaacggaggg cgacgcttcg agatcggggt aagcggatgc tcccgggtag cggcgaccct
     3181 ctcgaagacg cgtacgcagg gggagtgatc gctgcgaacg tcgcactacc gttcgacgga
     3241 cgagtcccat cgaccgggga cggcgagccg ggcgcgttcg gtccgagcac cctggtggcc
     3301 gagccaaggt cggcagcgac gcatcagcgc ttcccgtcct tggccagcga tggggacagc
     3361 gccctcatcg catggatgga gggcgcgcca ggtgaggaat cggtgcgcgc attcatcacg
     3421 gaccttgcga gcccggcaga agaggtcgag ctcgacgaag cgggtgacgt cgtgcggcga
     3481 ttgcctcggg tggccgttgc tgccggtcga atggccgtcg tttgggaaga ggagtcgcgc
     3541 gatcagacgg ctcgggtggt agtcgcaacc cgtgccggag aatcctggag gcaaatggcg
     3601 atcaccgagc cgggtgttgg cacagcctgg gagccggacg ttgcgattga cccggtcacc
     3661 ggcaattacc acgtggcctg gctggacctt cgtaacggtg gacgaccgaa gccttgggtt
     3721 gcccggagcg aggacgggca aagctgggag acgcgccagg tcgaccccgg caacgccatc
     3781 gtcgacaacc cgcgaggaga cgccgcgttt cctaagatct cggcacgcga tggttcggtg
     3841 ttcgtggcgt tctcggattt tagggagttc agttgggacg tctatctgtc ggcgagcggg
     3901 gatggtggat tg
//
LOCUS       DASRZF010000035        10130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3808825, whole genome shotgun sequence.
ACCESSION   DASRZF010000035 DASRZF010000000
VERSION     DASRZF010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 10130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10130
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3808825"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VFG22_01600"
     CDS             <1..181
                     /locus_tag="VFG22_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414961.1"
                     /translation="IEVLVSWLDVPASRVQELLTRLDAQGLVDQARCRLSMQGLVLAV
                     SMNGAQKLAKHSIAA"
     gene            229..312
                     /locus_tag="VFG22_01605"
     tRNA            229..312
                     /locus_tag="VFG22_01605"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:263..265,aa:Leu,seq:cag)
     gene            440..1102
                     /locus_tag="VFG22_01610"
     CDS             440..1102
                     /locus_tag="VFG22_01610"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET6414962.1"
                     /translation="MRVAPRGCVLGQSRKRREGLTLVELMVVLAVMGILVSAALPSMG
                     EGFADRRVAMVARDVVSLFARARYMSMAYGRAHQVLYTNDSGTGLTSQAYAFETLRGT
                     GGACSLTTFENSGGIALTDLDCGNNWRCVDHLYANTYDANPGDNDLVRVDGWKDTTFC
                     YEAGSNNQVFVDTVLFSNWQAANAQAGFGFVVYRELDGKAIGVARKVLIPLGTGSPRV
                     LR"
     gene            1099..1725
                     /locus_tag="VFG22_01615"
     CDS             1099..1725
                     /locus_tag="VFG22_01615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET6414963.1"
                     /translation="MNGMRKTRPSGFTLIEVMIAIGIMTVGSLGILAMHQAVSNANRS
                     AHEMNTALAITERWIERIDRDALSWTEQGLATSALSGTDYLSPLASTTTGTDWFTPAP
                     SNTDESYGFDFFGLDTREKDEIKYCTNLKLSWLRQGNSARVDIRTFWYRAGHMLGTAQ
                     HSKWVSGADFRGAECDAATADGWDLAEAPNVDTVFTSTVVTWLRKVAP"
     gene            1722..3053
                     /locus_tag="VFG22_01620"
     CDS             1722..3053
                     /locus_tag="VFG22_01620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET6414964.1"
                     /translation="MRNSGFTLIELMVAMVIGLTAITSVYALGSAMSRQFHEEQRMAT
                     SQGTSRVAIMELRRDVSRAGLFGSPNGTNEPTCDASPMQLPLLGGGSGDLGAFQYYPD
                     ADLATLDPDSVNTSVHADRLRILTSLYLSDQLLVDSASVDGATIVLQSGNQAYRRTFS
                     WGQTAGPFTSGSPPTYLDGDLDWDSAWAGETASWKGIGQKGARAFQTGSVLHIETPAG
                     RHFFRSVFGKDGNAQDEVRIKLDTALPIGSACLPGAAEGATLAPLQWVEYALVDPFDS
                     SEVGADFMDFDGMFYIDLDPSNPSFALKNTTASDLLESPNVVLVRRVLNASTGNVLPN
                     TTQIIAEFVSNFEVSFIVDTSSGNAGAPTLDLLTRSEATTEINDNPQQVRSVIIDLGI
                     RSPLEDPSIDYASNTDAGTRFEVNKDQTGSARVRKMRIEIPVMNVARRNLL"
     gene            3050..3730
                     /locus_tag="VFG22_01625"
     CDS             3050..3730
                     /locus_tag="VFG22_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414965.1"
                     /translation="MKHPRKQEGAALFVVLMIVMVGTASAVFAANTVSHEVRGTGFAR
                     QQLQTRNVARAGLIGALEWYDIFGPVAIRDLMLSPDSTEGVLYDTCTSTGGCYPEPPL
                     ANGRRAYRIHAAHFEALLTQDGGLTPGLIENLPIEADYSFGSTSAHSPVVVVDLYDEH
                     IVSTLAPGAAAQGGTKFKYMRTTLTARGRARVASDATESDDHGRERNETAADMRAYVV
                     SGPFVTGL"
     gene            3730..7329
                     /locus_tag="VFG22_01630"
     CDS             3730..7329
                     /locus_tag="VFG22_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414966.1"
                     /translation="MSASLHRQIAITSVLLASFASNAKVSAQDPDIRNIRPHIMLLVD
                     SSGSMERKPNCICTTPACLECLPVCAPGNYEQNRWSVVAQALTGEFSPYECNSDTRIG
                     GIYSGEYDEGYFLPHIQLPQEILSYSGSQSGNGVLDIYLERIKFGLMTFDAIGTLSDR
                     PPLVTQADFLTAPFPTESLGTKGMYSYADDRPFSFPGALSTYMLNNGARSSSAPEGGL
                     VSVGADSTAAMTATNASIQATLLGDAGLGKNPLRPFGATPTAALVTDLQYFLQTDADI
                     TQKTADPGPGDPYYDCRKRSAVLITDGFPNGDMRGNPVNCELLGQPVGATGCPYELVT
                     DTVSAMVSAGELDKFYVIGFALDGNPAQVAAVEALLNDIATVGDSTEAYLVADRAELV
                     TALSTILNEQNRGATSRTAPAVTGLTPGLIQSEFISGFNVSFDASDPWDGILERRRIE
                     CVDGFPETQTVVDSDRFQLTLNAQSVAPGDVEPWSADVGVSFAGGFSRNLWTVLPNDP
                     ADINSHLIGNGKTKITSLSNDAIDAPQDFSGLEIRDVPAGEFSKAISPEYFFGPGSTD
                     TAQRDTVVDWVHGVVGSGREDERLGDIYHSTPAIVGPLVDDFEDRSYNDWRLGLAHQK
                     SPDELEDLSTDGWQLNRRPRVIYASTNDGVIHAFLADDYPVNSFTVGNKLEEFSCAEN
                     KKAGTELWGFIPPMFLDDLDDLLSGGSKQWFADGSIQVRNLYDVRSFGQDDGFGGTTD
                     SPAGQTNVWRTVMFLSFRNGGAGMVALDVTNPCKPEFLWQFTDEDLGDTYGQPTAAQI
                     FLKDSDPTPLGNPGGTIVFQPRQAHGVVILPGGQGVEDAGASPCTFSSGAALPEGMDT
                     ASNVGITPRSDRRCWRGTSSTPSAIPKGRALYFIDLITGTVIRKLDENTFPAPLNGAV
                     AVFRGDTGTTGSVAYTVDADGVLWRIGIGSGDPEDWNAEALHDMYYADGYDEAEPTYY
                     APSLTVDAAGNIVILTGTGNIDVLDDASAKNRVVSITEKLSFDGNGFIDKVEGRLNWE
                     IELDPGEQMTGPMELFGGQVYFGTFKAGGGTAIDACPFGGSRIFGVGYLDDPTSAGTL
                     IPLLKNGLGNDTTNLDGSDIASLNNTLLVGLQVAQLPVCTSTNEQTLVDAFSGQTATL
                     AMPWATSGRQFKLMGHLSGSASTTGGLSIDILEESITAPEGFTVISGMAESLE"
     gene            7473..7889
                     /locus_tag="VFG22_01635"
     CDS             7473..7889
                     /locus_tag="VFG22_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414967.1"
                     /translation="MAEEATHKKISRDFDAQGRLLASDDYVVGIRLPRGSELVRQQSL
                     EHVYRVRAPIDKVLAYFGPMMITGNVERRGKGAIYKRASVRGAEVNPTKVDVSILEIG
                     ERATRISVTELPPPPAHIPSTAETKAAASESFRQLD"
     gene            complement(7909..8931)
                     /locus_tag="VFG22_01640"
     CDS             complement(7909..8931)
                     /locus_tag="VFG22_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414968.1"
                     /translation="MLRVSSLLLIAASAAGLGCAAQQSARTSDALFGSPVAALARDHA
                     PDLYAQAEIAWEKAEAALRRKDQQAADEHLTEAHLWLAAGAVEAERIQLDRELAELQL
                     EEERWARQLGRDQEASAAVARDISRYEARAVALREADRISAIEGSSAVSGATLDAVLT
                     RVRFNLALAEALGAPDARLSPLRDRAETMARKPSESAGSVEALLLSSEGLLGEIRGDW
                     PEPRPGSSTELVETAVVLGFVAGRAGNGVVIRSERFFLSSGQVSNATVKRFQGLVDAF
                     PHGPVACQVAVPELRSEAWTQRVAQLVARLRPMDDRARVSTSMIATQSLSAGAVQCTF
                     AAYKEP"
     gene            complement(8931..9356)
                     /locus_tag="VFG22_01645"
     CDS             complement(8931..9356)
                     /locus_tag="VFG22_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414969.1"
                     /translation="MRAALEALILVGVACAFSASSGLADEATSSAEAVQLQTRLARLE
                     ARGDAVHAKGALEQARRALQSASDAAGDDERVSRAEGIARAAMVLAERQLERRRSQDD
                     LATTQARLAAIREQADAQRRALETLMRDRASIARQEVQP"
     gene            complement(9367..9993)
                     /locus_tag="VFG22_01650"
     CDS             complement(9367..9993)
                     /locus_tag="VFG22_01650"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET6414970.1"
                     /translation="MGDQGDRKTVRAFQCRDYLFEIFDRMSSDRQCSVDYLINEAMRE
                     YARAREQTVAALDAQEMRGSAMGGGGSMAAPQRDPRPAPMLTPVPSRRPTVQPRVTAP
                     PPLPGHRPSDAASRQVLVLQFNGQKFPVGKDEFIIGRGARSADLAIRDGNISRRHAAV
                     VFHDGVYFMKDLGSTNGIEFAGQRVESKRIEEGDVYRICDYELRFTYQ"
BASE COUNT         1937 a         3295 c         3052 g         1846 t
ORIGIN      
        1 catcgaggtg ctcgtcagct ggctcgatgt tcccgcatcc cgtgttcagg agctattgac
       61 tcgactcgat gcgcaagggc tggtcgacca ggcccggtgc cgactctcga tgcaggggct
      121 cgtgctcgcg gtttcgatga acggggccca aaagctggca aagcattcga tcgcggcctg
      181 acctggcttg acagcccgga gagcaacaag tatctcggcg tgccttctgc ggaaatggcg
      241 gaattggcag acgcgcatga ttcaggttca tgtgtccgaa aggacgtgga ggttcgagtc
      301 ctcttttccg cacccagccg ttcatgacgt cggctctgta attttttcat agcgctcgca
      361 ggttattcat agctagcgaa gccctggctc tgagccaaaa tcaatctttt cagatactta
      421 gccgcttctt gattccggta tgagagttgc acctcgaggg tgcgtgcttg gccaatctag
      481 aaagaggcgt gaagggctga cgctggtgga gctcatggtc gttttggcgg tcatgggcat
      541 tctggtgagc gcggctcttc caagcatggg cgagggcttc gccgatcgcc gtgtggcaat
      601 ggtcgcgcgc gacgtcgtct ccctcttcgc ccgtgcgcgc tacatgtcga tggcgtacgg
      661 ccgggcgcac caggtcctct acacgaacga ctcgggaacc gggctcacca gtcaagccta
      721 cgcgttcgag acgctccgcg gcaccggtgg tgcttgctca ctcaccacgt tcgagaactc
      781 cggcggcatc gcgctcaccg acctggactg cggcaacaac tggcgctgcg tggaccatct
      841 ctacgccaat acctacgacg cgaacccagg ggacaacgat ctggttcgag tggacggctg
      901 gaaagacacc acgttctgct acgaggctgg ctccaacaac caggtcttcg tcgacacggt
      961 cctgttctcg aactggcagg ctgccaacgc gcaggccggc tttggcttcg tcgtctatcg
     1021 cgagctcgat gggaaggcga tcggagtcgc gcgaaaggtc ctgatcccgc tcggaacggg
     1081 gtccccacgg gtgctgcgat gaacggaatg cgcaagacga gaccgtccgg cttcaccctc
     1141 atcgaagtca tgatcgcgat cggcatcatg accgtcggct cgctcggcat cctcgcgatg
     1201 catcaggcgg tgagcaacgc aaaccgatcc gctcacgaga tgaacaccgc actggcgatc
     1261 accgaacgtt ggatcgaacg catcgatcgc gatgcgttgt cttggacgga acaaggactc
     1321 gccacgagcg cgctctccgg tacggactac ctgtccccgc tggcctcgac caccactgga
     1381 accgactggt tcacgccggc accttcaaat acggatgaat cgtacggctt cgatttcttc
     1441 ggtctcgaca cccgggagaa ggacgagatc aagtactgca cgaacctgaa gctcagctgg
     1501 ctcaggcaag gaaactccgc gcgcgtcgac attcgcacct tctggtatcg cgccggtcac
     1561 atgctcggaa ctgcgcagca cagcaaatgg gtctccggcg cagactttcg gggcgcagaa
     1621 tgcgatgccg cgaccgcgga tggctgggac ctcgcagaag ctccgaacgt cgacacggtc
     1681 ttcacctcca cggtcgtcac ctggttgaga aaggtggccc catgagaaac agcggtttca
     1741 ccctgatcga gctgatggtc gcaatggtca tagggctcac ggcgatcact tccgtgtatg
     1801 cgctgggctc cgcgatgagc cggcagttcc acgaggaaca gcgcatggcg acctcgcaag
     1861 ggacttcgcg cgtcgcgatc atggagcttc ggcgcgacgt gtcccgggcc gggctctttg
     1921 gctcgccaaa cggaacgaac gagccgacct gcgacgccag ccccatgcag cttccgctcc
     1981 tcggcggcgg cagcggcgac ttgggtgcgt ttcagtacta tcccgacgcg gaccttgcga
     2041 ccctcgaccc ggacagcgtg aacaccagcg tccatgcgga tcgacttcga atcctgacca
     2101 gcctctacct gtccgaccag cttctggttg actccgccag cgtcgatggg gccactatcg
     2161 ttcttcaatc gggcaatcaa gcctaccgcc gcaccttcag ctggggacag accgcggggc
     2221 cattcaccag tggcagccca cccacgtatc tcgacgggga tctggactgg gacagcgcat
     2281 gggcagggga aaccgcgagc tggaagggta tcggccagaa aggcgcgcgg gcgttccaaa
     2341 ccggctcggt gctccacatc gaaaccccgg cgggtcgtca cttctttcgc tcggtcttcg
     2401 gcaaggacgg caacgcgcaa gacgaagtgc ggattaagct cgatacggca ctgccgatcg
     2461 gcagtgcctg ccttcctggc gcggcggagg gcgcgaccct cgctccgctg caatgggtcg
     2521 aatacgcact ggtggaccca ttcgacagca gcgaggtcgg cgcagatttc atggacttcg
     2581 acgggatgtt ctacatcgac ctcgatccct cgaacccctc ctttgcgctc aagaacacga
     2641 ccgcgagcga cctcctcgaa tcacccaacg tagttctagt ccgacgcgtg ctcaacgcgt
     2701 cgaccggcaa cgtgcttccg aacaccacac agatcatcgc cgagttcgtc agcaatttcg
     2761 aagtcagctt catcgtcgac acgagctccg gcaatgcggg agctccgacg ctggatttgc
     2821 tcactcgttc ggaggccacc accgaaatca acgacaaccc ccaacaggtc cgctcggtca
     2881 tcatcgacct cggaatccgt agccccttgg aggacccgtc gatcgactac gcctcgaaca
     2941 cggacgcggg gacccgattc gaggtcaaca aggatcagac cggctcagcc cgggttcgga
     3001 agatgcggat cgaaattccg gtgatgaacg tggccaggag gaatctctta tgaagcatcc
     3061 tcgcaaacag gaaggcgccg ccctattcgt ggtgctgatg atcgtgatgg tgggcactgc
     3121 gagcgccgtg ttcgccgcaa acacggtctc gcacgaggtc cgaggcacag ggttcgcgcg
     3181 ccagcaactc cagacgcgga acgtggctcg cgcgggcttg atcggagcgc tcgaatggta
     3241 tgacattttc gggccggtcg cgatccgaga tctgatgttg tccccggaca gcaccgaggg
     3301 cgtcttgtac gacacttgca cgagcacggg cggttgttac cccgagccgc ccctcgcgaa
     3361 cgggcggcgc gcgtatcgga tccatgccgc gcacttcgag gcgctgctga ctcaggacgg
     3421 cggcctcacg cctgggttga tcgaaaacct gccgatcgag gcggactact cgttcggctc
     3481 tacctcggcg cactcgccag tcgtggtggt cgatctctat gacgagcaca tcgtgagcac
     3541 gctcgcgccc ggcgcagcgg ctcagggtgg aaccaagttc aagtacatgc gcacgacgct
     3601 gaccgcccgc ggtcgtgccc gcgtggcgag cgacgctacg gaatccgacg accatgggcg
     3661 ggaacggaac gagaccgctg ctgacatgcg cgcctatgtc gtcagtggcc cgttcgtgac
     3721 ggggttatga tgagcgcttc acttcaccga cagatcgcaa tcacgagcgt gctgctcgct
     3781 tcgtttgcct cgaacgccaa ggtctccgcc caggacccag acattcggaa catccgtccg
     3841 cacatcatgc ttctggtcga cagctccggt tcgatggagc gaaagcccaa ctgcatttgc
     3901 acgacacccg cttgcctcga atgcctaccc gtctgcgcgc cggggaacta cgagcagaac
     3961 cgctggtcgg tcgtcgccca ggctctcacc ggcgagttct cgccttacga gtgcaactcc
     4021 gacacgcgta ttggcggcat ctacagcggc gaatacgacg agggatactt cctcccacac
     4081 attcagttgc cgcaggagat cctctcgtat tccggctcac agagcggaaa cggcgtcctc
     4141 gatatctacc tcgagcgaat caagttcggt ctgatgacct tcgatgcgat cggcaccttg
     4201 agcgatcggc ctccgctcgt cacccaggcc gacttcctga ccgcgccttt tccgaccgaa
     4261 tcgctcggca ccaagggcat gtattcctac gcagacgacc gtcctttctc gttcccgggc
     4321 gccttgagca cgtacatgct caacaacggg gcgcgctctt catctgcccc cgagggtggc
     4381 ctggtcagcg ttggcgcgga cagcaccgcc gcgatgacgg ctacgaatgc ctcgattcag
     4441 gccaccttgc tcggtgacgc aggtcttgga aagaacccac tccgcccgtt cggcgccacg
     4501 cccaccgcag cgctcgtcac cgatctccaa tacttccttc aaacggatgc ggacattacc
     4561 cagaagaccg ccgatccagg cccgggcgac ccgtactacg attgccggaa gcgcagcgcc
     4621 gtcctgatca ccgacggctt tcccaatggc gacatgcgcg ggaacccggt gaactgcgag
     4681 ctgctcggtc agcccgtggg cgcaaccggc tgcccttatg agctggtcac cgacaccgtc
     4741 tcggcgatgg tctccgccgg tgagctcgac aagttctacg tcatcggctt tgccttggac
     4801 ggcaatccag cgcaggtcgc ggctgtcgag gcgctcctca acgacatcgc aacggtaggc
     4861 gacagcactg aagcgtacct cgtcgccgac cgcgccgagc tcgtgaccgc actcagcacg
     4921 attctcaacg agcagaaccg aggcgccacc agtcgtaccg ctccagcggt caccggtctc
     4981 acaccagggc tcattcaatc ggagttcatc tcgggcttca acgtctcctt cgatgcctca
     5041 gacccatggg acgggatctt ggaacgacgg cgcatcgagt gcgtcgatgg cttcccagaa
     5101 acccaaaccg tcgtcgattc ggatcgcttt cagctgacgc tcaacgctca gtcggttgcg
     5161 ccgggtgacg tggagccatg gagtgcagac gtcggcgtga gtttcgcggg cggcttctcg
     5221 cgaaacctct ggacggttct cccgaatgat cccgccgaca tcaactctca cttgatcggc
     5281 aacggcaaga ccaagatcac ttcgctttcg aatgacgcga tcgatgcgcc gcaagacttc
     5341 agcggtctcg agattcggga cgtgcctgcc ggcgaattca gcaaggccat ctcgccggaa
     5401 tacttcttcg gcccgggcag cacggatacg gcgcagcgcg acacggtcgt cgattgggtg
     5461 cacggcgtcg tcggaagcgg ccgcgaagac gagcggctcg gcgacatcta tcattcgaca
     5521 ccggcgatcg tcgggccgct ggtggacgac ttcgaagacc gctcttacaa cgactggcgc
     5581 cttggcttgg cacatcagaa gagcccggat gagctcgaag atctttcgac cgacggatgg
     5641 cagctcaacc ggcgtccacg ggtgatctac gcgtcgacga acgatggcgt cattcacgcg
     5701 ttcctcgccg atgattaccc ggtgaactcc tttacggttg ggaacaagct cgaagagttc
     5761 tcctgtgcgg agaacaagaa ggcgggtacc gagctttggg gcttcattcc gcccatgttc
     5821 ttggacgacc tcgacgacct gctctccggc ggcagcaagc agtggttcgc cgatggcagc
     5881 attcaggtgc gcaacctcta cgacgtgcgc tcgtttggtc aggacgatgg gtttggagga
     5941 accactgact ccccggccgg tcagacgaac gtctggcgca cggtgatgtt cctgagcttc
     6001 cgaaacggcg gcgccggcat ggtcgccttg gatgtgacga acccgtgcaa gcctgagttc
     6061 ctctggcagt tcacggacga agatctcggt gacacctatg gtcagccgac ggccgctcag
     6121 attttcctga aagacagcga tccgaccccg ctcgggaacc cgggcggtac gatcgtcttt
     6181 caacctcggc aggcgcatgg cgtggtgatt ctcccaggcg gacagggcgt agaagacgca
     6241 ggggcaagcc cttgcacatt cagctcgggc gcagcgcttc cggaaggcat ggacaccgcc
     6301 agcaacgtcg gcatcacgcc tcgctccgat cgccgatgct ggcgcggcac gagctcgacg
     6361 ccctccgcca tcccgaaggg aagagcgctc tatttcatcg atctcatcac cggcacggtc
     6421 attcgcaagt tggacgagaa cacgttcccg gcgcccctca atggtgcggt cgccgtgttc
     6481 cgcggcgata ccggcaccac gggaagcgtc gcttacacgg tcgatgcgga cggggtgctc
     6541 tggcgcatcg gcatcggctc gggtgacccc gaagactgga acgccgaggc gctccacgac
     6601 atgtactatg cggacggcta cgatgaggcg gaaccgacct attacgcgcc ctccctcact
     6661 gtcgacgccg ccggcaacat cgtgatcctc acgggaacgg gcaacatcga cgtgctcgac
     6721 gacgccagcg ccaagaaccg cgtggtgtcg atcacggaga agctcagctt cgacggcaac
     6781 ggcttcatcg acaaggtcga gggacgactc aactgggaga tcgagctcga tcccggcgag
     6841 cagatgaccg gcccgatgga gctcttcgga ggccaggtct actttggcac cttcaaggcc
     6901 ggcggcggta cggcgatcga tgcctgccca tttggcggca gccggatctt cggcgtgggc
     6961 tacctcgacg atccgacaag cgcaggcacg ttgataccct tgctcaaaaa tggcctcgga
     7021 aatgacacga ccaacctcga cggaagcgat atcgcttcac tcaacaacac cttgctcgtg
     7081 ggcctgcagg tcgcgcagct tcccgtgtgc acgtcgacca acgagcagac gctggtcgat
     7141 gctttttcgg gccaaacggc tacactcgcc atgccctggg ccaccagcgg acgccagttc
     7201 aagctgatgg gccatctcag cggctccgca agcaccaccg gaggactgtc gatcgacatc
     7261 ctcgaggagt cgatcaccgc accggagggc ttcacagtca tctcgggcat ggccgaatcc
     7321 ctggaatgat cggtagagcg atgatcacgg ttgcaacgat gcctcccctg cgatttttcg
     7381 ctctgagtct cgcgataacg ctgactgcgt gctcgtccaa atcccgggaa agtgccgagc
     7441 cgcagcattc ggatgctcca gcaaatgccc gcatggcaga agaagcgacc cacaagaaga
     7501 tctccagaga cttcgatgct cagggacgcc tactcgcttc ggatgactac gtggtgggga
     7561 tccgacttcc gaggggcagc gagctcgtgc gtcagcaaag cctcgaacac gtttatcgag
     7621 tccgggctcc gatcgacaag gtcctcgcct acttcggtcc aatgatgatc acgggcaacg
     7681 tggagcgtcg tggcaaaggc gcaatctaca agcgcgcaag cgttcgcggc gctgaagtca
     7741 atcccacgaa ggtggatgtc tcgattctcg agatcggcga acgggcgaca cggatctcgg
     7801 tgaccgagct tcctccgcca cccgcgcaca tcccgagcac tgcggagacc aaggccgctg
     7861 caagcgagtc gttccggcag ctcgactgag ccgcaagtgg actgtcgctc agggctcttt
     7921 gtaggcggcg aacgtgcact gcacagcccc ggcgctcagc gactgagtcg ctatcatgct
     7981 cgtggagact cgcgcccgat cgtccatggg tcgcagtcgt gcgacgagct gagccacgcg
     8041 ttgcgtccag gcctcactgc gaagctcggg caccgcgacc tgacaggcga ccggaccgtg
     8101 gggaaacgcg tcgaccaagc cctggaaccg cttcaccgtc gcgttcgaga cctgaccgct
     8161 cgataagaag aagcgctccg agcgaatcac gacgccatta cctgctcgac cggctacgaa
     8221 ccccagaacc accgccgtct cgacgagctc cgttgaagaa cccggtcgcg gctccggcca
     8281 gtctccgcgg atctccccga gcagcccctc gctgctcagc agcagggcct cgactgatcc
     8341 ggccgactcg gagggcttcc gcgccatggt ctccgcacgg tcacgcaacg gcgagagccg
     8401 ggcatcgggg gctcccaagg cctcggccag cgcgagattg aaacgcacac gtgtaagaac
     8461 tgcgtcgagc gtcgccccgc tgacggccga gcttccttcg atcgccgaaa ttcgatcggc
     8521 ctctcgcagc gcaaccgctc tcgcctcgta acgcgagatg tctcgggcga ccgccgccga
     8581 ggcttcctgg tcgcggccga gctgcctcgc ccaacgttcc tcttcgagtt ggagctcggc
     8641 gagctctcga tcgagctgga tccgctcggc ctcgaccgcg ccggcagcca gccagagatg
     8701 ggcctccgtg aggtgctcgt cggccgcttg ttggtccttg cgccgcagcg cggcttcggc
     8761 cttctcccat gcgatctctg cttgcgcata gagatcgggc gcgtgatccc gtgcgagggc
     8821 agcaacggga gaaccgaaaa gagcatcgga agttctcgcc gactgttgag cggcacatcc
     8881 gagaccagcg gccgaggcgg cgatcagcag caacgacgaa acgcggagca tcagggctgg
     8941 acttcctggc gggcaattga ggcgcgatcg cgcatcaagg tctcgagagc tctcctctgc
     9001 gcatcggcct gctcgcgaat cgcggcaaga cgcgcttggg tggtggcgag gtcgtcctga
     9061 gatcgccgcc tctcgagctg gcgctcagcc agaaccatgg ccgcgcgggc aatcccttcg
     9121 gcgcgcgaaa ctcgttcatc gtctccggca gcgtccgaag ctgattgcag cgcacgtcga
     9181 gcctgctcga gcgcgccctt ggcgtgaacc gcgtcgcctc ttgcttcgag ccgcgcaagc
     9241 cgggtttgga gctggacggc ttcggcgctc gaggtggcct cgtctgctag ccccgaagac
     9301 gcggaaaatg cgcaagccac cccgacgaga atcaaggcct cgagcgcggc acgcacccaa
     9361 ctcggctcac tggtacgtga agcggagctc gtaatcgcag atgcgataca cgtcgccttc
     9421 ttcgatgcgc ttggattcga cacgttgacc cgcgaactcg attccattcg tgctgcccaa
     9481 gtctttcatg aagtacacgc catcgtggaa cacgactgcg gcatgtcgtc gcgaaatgtt
     9541 gccgtcgcgg atcgcgagat ctgcactccg ggcgcctcgc ccaatgatga actcgtcttt
     9601 tccgactgga aacttctggc cgttgaactg aagcaccagg acctggcgac tcgctgcatc
     9661 ggacggccgg tggcccggaa gaggtggcgg cgccgtcacc cgaggctgca cggtcggacg
     9721 ccgtgacgga accggggtaa gcatcggtgc aggacgggga tctctttgcg gcgcggccat
     9781 cgacccacca ccgcccatcg cggagcctcg catctcttgc gcatccaatg cggccacggt
     9841 ctgctcgcgg gcgcgtgcgt actctcgcat cgcctcgttg atcagatagt cgaccgaaca
     9901 ttggcgatcc gagctcatcc gatcgaagat ctcgaagagg tagtcgcggc actgaaaagc
     9961 ccgcacggtt tttctgtcgc cttgatcacc catcgattcg actactcact tttgtcaccc
    10021 tgttcgcgcg tccgaagcgc cgttatggct gcgtcagcaa catccccaac cgttttcacg
    10081 ggcgggttga gttctgacca tccctgtccc gaaactgttt ctccatccga
//
LOCUS       DASRZF010000036         2338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152 k141_23867,
            whole genome shotgun sequence.
ACCESSION   DASRZF010000036 DASRZF010000000
VERSION     DASRZF010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23867"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VFG22_01655"
     CDS             complement(<1..596)
                     /locus_tag="VFG22_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414971.1"
                     /translation="MTARAIGVFALVLAMASPALAGPKNSVIGGKKLEQDMVHSGGVG
                     YPSIMYEWWNKGKKNLDWGLSGELVYGPWGAAGIERYSFNGFGVRDRYIKIGLGVNGL
                     LRWNLATKERPKVTNDIAIFVRPGVLISGNTFNTFTFGIRAEVGAPVSIDVHERVSVV
                     VGGFIPFNYFINKDFDNAAWIPLLVRMGVEIKANENVEP"
     gene            769..1392
                     /locus_tag="VFG22_01660"
     CDS             769..1392
                     /locus_tag="VFG22_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HET6414972.1"
                     /translation="MRRAVFLFSLGLAVAGCKNPGAEVTAASVEPAAEAKEAMPAGAD
                     PTAGALAINPSNSKIEFVGAKITASHPGGFTDFAGTLELGDPLEKSAISVTIQTASLY
                     ADKEKLTKHLKSADFFDVERFPTATFRSTEIKKEGDGHVISGDLTLHGVTRQVSFPAS
                     VSASETQVSASAEFSINRQDFGIAYPGMPDDLIRDLVVIKLSLALPR"
     gene            complement(1389..>2338)
                     /locus_tag="VFG22_01665"
     CDS             complement(1389..>2338)
                     /locus_tag="VFG22_01665"
                     /inference="COORDINATES: protein motif:HMM:NF023500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3556 domain-containing protein"
                     /protein_id="HET6414973.1"
                     /translation="AVMQSNSPLTRIGSLRKIFYRNYPEYLRPSRLTHLMAHAGTAVE
                     LMFPVVLVLSDGGPSTTVALAVMVLFHTFITSSIPMGVPIEWNVIMVYGAFVLFGHYA
                     DVSAFSIGSPLLIAYLAFALLAVPLFGNLVPSRVSFLCSMRYYAGNWAYSVWLFKGDC
                     SKKLDLHLTKAAPNLHAQLEKFLDEDTITATVSKVLGFRAMHLHGRCLQSLLPKAVDD
                     IDAYEYLDGELVAGIVIGWNFGEGHLHNMQLLRSIQEQCQFEEGELRCIFVESQPMGQ
                     GTHSWTIADAATGVRETGKIVVKDLLDLQPWPPLETRKP"
BASE COUNT          492 a          681 c          702 g          463 t
ORIGIN      
        1 ggctctacgt tttcattagc cttgatttcg acgcccatcc gtacgaggag cgggatccag
       61 gctgcgttat cgaagtcctt gttgatgaag tagttaaacg ggatgaagcc gccgaccacc
      121 acgctcaccc gctcgtggac atcgatcgac accggcgcgc caacctctgc cctgatgccg
      181 aacgtgaaag tgttgaatgt gttgccagaa atgagcacgc ccgggcgaac gaagatcgcg
      241 atgtcgttgg tgaccttggg acgctctttg gtagcgaggt tccagcgaag gaggccgttc
      301 acgcccaaac cgatctttat gtaccgatct cggaccccaa acccgttgaa actatagcgt
      361 tcgatacccg ccgcacccca agggccatag accagctcgc cgctcagtcc ccagtcgaga
      421 ttcttcttac ccttattcca ccattcgtac atgatgctcg ggtaaccgac accgccgctg
      481 tggaccatgt cctgttcgag cttcttaccg ccgatgaccg aattcttcgg tccggctagc
      541 gcagggctcg ccatcgcgag caccaatgcg aatacaccaa ttgctcttgc tgtcattatt
      601 ccagctgctc cttccccgta aggtggccga accttagagc acaaccgccc accttgccag
      661 cgattatcaa ggcgaaccca ggtggacaca agtttcgtga ttcacgaatg attcctgtcg
      721 cttgacccgc cggcaaaggt caccataagc ggggctcgag gaggttccat gcgtagagct
      781 gtgtttttgt tctctcttgg tttggccgtt gctgggtgca agaaccccgg tgcggaggtg
      841 acggctgcca gcgtcgagcc cgccgcggag gcgaaagaag cgatgccggc tggggctgac
      901 ccgacagccg gggctctggc gatcaaccca tcgaacagca agatcgagtt cgtcggcgcc
      961 aaaatcaccg cgagccaccc cggtggtttc accgacttcg cgggcacgct cgagctcggt
     1021 gatccgctcg agaagagcgc aatctcggtc acgatccaaa cggcatcgct ctacgcagac
     1081 aaagagaagc tcaccaagca cttgaagtcg gccgacttct tcgacgtgga aagattccca
     1141 accgcgacct tccgctcgac cgagatcaag aaggagggtg atgggcacgt gatcagcggc
     1201 gatctcaccc tgcacggggt cacacgacag gtcagctttc ccgccagcgt gagcgccagt
     1261 gaaactcagg ttagcgcaag cgcggagttc tcgatcaatc ggcaggactt cgggatcgcg
     1321 tatccgggga tgcccgacga tctgattcgt gatctcgtcg tcatcaagtt gtcgctagcg
     1381 ctgccgcgct agggcttgcg ggtctctagc gggggccagg gctgcaagtc gagcaggtct
     1441 ttgaccacga tcttgccggt ttcccggacc ccggtcgccg catcggcgat ggtccaggaa
     1501 tgcgtgccct gtcccattgg ctgtgactcg acgaagatgc aacgaagctc gccttcttcg
     1561 aactgacact gttcctgaat cgagcgcaga agctgcatgt tgtgcagatg gccctcgccg
     1621 aagttccatc cgatgacgat tccggcgacg agctcgccat ctagatactc ataggcatcg
     1681 atgtcgtcga cggcctttgg caggagcgat tgcagacagc ggccgtgaag gtgcatcgcg
     1741 cggaagccga ggactttgga cacagtcgcc gtgatggtgt cctcatcgag gaacttctcg
     1801 agctgtgcgt gcaggttggg ggccgccttg gtgaggtgca aatcgagttt tttggagcag
     1861 tcgcccttga agagccaaac gctgtacgcc cagttccccg cgtagtagcg catcgagcat
     1921 agaaacgaga ctcgtgatgg gacgaggttt ccgaagaggg ggaccgcgag cagtgcaaac
     1981 gcgaggtatg cgatgagcaa gggcgagccg atgctgaagg cgctcacgtc cgcgtagtgc
     2041 ccgaatagaa cgaacgcgcc gtataccatg atgacgttcc actcgatggg aacacccatg
     2101 ggtatactgc tcgtgatgaa ggtgtggaag agcaccatga cggccagcgc aaccgtcgtt
     2161 gaagggcctc cgtcactgag cacgagcacg acgggaaaca tgagctcaac ggcagtgccg
     2221 gcgtgcgcca tcaggtgcgt gagccgagag gggcgcaggt attcagggta gtttcggtag
     2281 aaaatcttgc gcaatgaacc gatgcgcgtg agcgggctgt tgctctgcat gaccgcca
//
LOCUS       DASRZF010000037         2021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4840486, whole genome shotgun sequence.
ACCESSION   DASRZF010000037 DASRZF010000000
VERSION     DASRZF010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4840486"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="VFG22_01670"
     CDS             complement(<1..830)
                     /locus_tag="VFG22_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414974.1"
                     /translation="MKRTVLAALLLLSSACASSKSSTNDGSTIGKGIDVETMRPPEAA
                     QVVKEFQAAYGIPPLPPPDEPVTSMAQVLDIVRQDRLSEFDEARRFASSRMGAEALTI
                     RGYLELSYADALMTAAWVLEREQRQDQTELRQLSMARPLESGKDAQQDGARAARLQAK
                     IADTRKVVRALRVLSQEPLDSGADLIKQAIAQDPKSQMAYLANANYYRLRGNWLEYDR
                     MMLYADEGQADPPVRTFLRAMEAFERYVDPDRCVKLLKETLAMRPDLVRAQADLVLVS
                     "
     gene            complement(914..>2021)
                     /locus_tag="VFG22_01675"
     CDS             complement(914..>2021)
                     /locus_tag="VFG22_01675"
                     /inference="COORDINATES: protein motif:HMM:NF016819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HET6414975.1"
                     /translation="PRGNGSGDPRNRGDLRRAHRVRAREQAMNRALIWVLLCLATTVS
                     FGSRANAHESRPLYVEVTENAPLVFSIRWKIPPSIEIANAPEVSMTGGCTSTTPPRPR
                     GTRASLQHRSYQCETDPAGSPLRIRYALYNPSVSALVRVTRLSGEKYSIVASPEEGEV
                     ILPESESFGSVARDYLALGVRHILEGYDHLLFLVCLMLIAGTGRRILITITGFTLAHS
                     VTLVLSALGLVQVPVAPVEAAIALSIVFLALEIVRGNPQSLTYRYPIAVSSSFGLLHG
                     FGFAAVLGETGLPQTEIPAALLFFNLGVEVGQLVFVLGVILVYQLARFGARKIAGADL
                     ALDRLRPFETPAAYAVGILASFWMIQRVATFWMS"
BASE COUNT          388 a          613 c          658 g          362 t
ORIGIN      
        1 tcgctcacga ggacgaggtc cgcttgcgcg cggacgaggt ccgggcgcat ggcgagcgtt
       61 tccttgagaa gcttcacgca gcggtcgggg tcgacgtatc gctcgaacgc ttccatcgcg
      121 cggagaaagg tgcggaccgg aggatcggct tgcccctcgt cggcgtagag catcatccgg
      181 tcgtactcga gccagtttcc gcgcaagcgg tagtagttgg cattggcgag atatgccatc
      241 tgtgatttcg ggtcctgcgc aatcgcctgc ttgattaggt cagccccgga atcgaggggc
      301 tcctgagaca atacgcggag tgcgcggacg accttgcggg tgtcggcgat tttggcttgg
      361 agcctggcgg cacgggcgcc gtcttgctga gcatcctttc cgctttcgag tggtcgcgcc
      421 atcgatagct gccgaagctc ggtttgatct tgacgctgct cgcgttccag aacccaggcg
      481 gcggtcatca gcgcatctgc atagctgagc tcgaggtagc cacggatggt caacgcttcg
      541 gctcccattc ggctcgatgc aaatcgcctg gcctcgtcga actccgaaag gcgatcttgc
      601 cggacgatgt cgagcacctg cgccatggag gtcacgggct cgtcgggagg aggcaagggc
      661 gggatgccgt acgcagcctg gaactctttc acgacctgcg ccgcttcggg tggacgcatc
      721 gtctcgacgt cgatgccttt gccaatggtc gacccgtcgt tcgtcgaaga cttcgaggac
      781 gcgcaagcgc tcgagagcag cagcagggct gcgagaaccg ttcgtttcat ggcgcggagg
      841 ctagcacagc cagcgatggg ggccccagat tcgcatccaa ggcctggatt ccggcgcgac
      901 gcaccctccc gactcagctc atccagaacg tcgccacgcg ctggatcatc cagaacgagg
      961 cgaggatgcc gacggcgtag gcggctggcg tctcgaatgg gcgcagtcga tcgagcgcaa
     1021 ggtcggctcc ggcaattttc cgggccccaa atcgtgcgag ttggtagacg aggatcacgc
     1081 cgaggacgaa gacgagctgc cctacctcga cgcccaggtt gaaaaacagc aaggcagcag
     1141 ggatctcggt ctgcggaagc cccgtctctc cgagcacggc cgcgaagcca aagccatgca
     1201 ggagcccgaa tgacgaagaa accgcgatcg ggtagcggta cgtcaggctc tgcgggttgc
     1261 cgcgcacgat ctcgagcgcg aggaaaacga tgctgagcgc gatagcggct tccaccggcg
     1321 ccacaggcac ctggacgagg cccagcgcgc tcagcacgag cgtgaccgag tgggccaggg
     1381 tgaaccccgt tatcgtgatc aagattcgcc ggcctgttcc cgcgatcaac atcagacaga
     1441 ccagaaacaa gagatgatcg tagccctcga ggatgtgccg aacaccgagt gccaggtagt
     1501 cccgggcaac gcttccaaag ctctcggact cgggaaggat gacttcgcct tcctctggag
     1561 aggcgacgat ggagtacttc tcccctgaga gcctggtcac gcgaacgagc gcagaaaccg
     1621 atgggttgta gagtgcgtag cgaatccgaa gcggggagcc ggcagggtcg gtctcacact
     1681 ggtaactgcg gtgctgaagg cttgctcgcg tgcctcgggg ccttggcgga gtcgtcgacg
     1741 tgcaccctcc ggtcatgctc acctccggcg cgttcgcgat ctcgatcgac ggaggaatct
     1801 tccatcgaat cgaaaacacg agcggcgcat tctcggtgac ttcgacgtag agcgggcggc
     1861 tttcgtgcgc attcgctcga gaaccaaagc tcaccgtggt cgcaaggcac agcaagaccc
     1921 agatcaaagc acgattcatg gcttgctcgc gcgctcgtac tcgatgcgca cgtcgtaggt
     1981 ccccacgatt tcgcggatcg ccgcttccgt ttcctcgcgg a
//
LOCUS       DASRZF010000038        10900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3121113, whole genome shotgun sequence.
ACCESSION   DASRZF010000038 DASRZF010000000
VERSION     DASRZF010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 10900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10900
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3121113"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="VFG22_01680"
     CDS             complement(<1..666)
                     /locus_tag="VFG22_01680"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HET6414976.1"
                     /translation="MIVRHQRESASNRGFRKLARYIQGRGAKPRTTWFYSANLPGVGG
                     RDDLELACALVDAVQSQNTRAGSKRTYHLVISLHPDDRRLERGELQRVVDRLVDTLGF
                     SEHQHVAVRHNDTDHEHIHVAINKIHPETFRIHSPAWDHQKLFTGARALERELGLIPL
                     RSKTRDREKIPERAIDCEAHQGIHSFARWARENLRPALRSSELRTWDDVHDVYSRLGV
                     VARP"
     gene            complement(663..1025)
                     /locus_tag="VFG22_01685"
     CDS             complement(663..1025)
                     /locus_tag="VFG22_01685"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HET6414977.1"
                     /translation="MTAVPGAHVAAKKAKSRIVSFRVDEDDFARLDALARQTSCSRGE
                     VLRRCLRGARLESTLDAQALSEVSRLHANLNRIGGLLKLAIREGVSDPSSHRRLNLEL
                     IDSVMNVRRAVSRLAGQR"
     gene            complement(1074..2600)
                     /locus_tag="VFG22_01690"
     CDS             complement(1074..2600)
                     /locus_tag="VFG22_01690"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET6414978.1"
                     /translation="MAKQKKRSTTGIRWDEAKQRYRISYKDGEGRWRREHAGRSFEAA
                     KRKLSTRKRQVREGTLDLERGVGPNVTLAEFVKRWEAQQAVGRRRNLDREVRQLRLHV
                     LPTLGRRPIASITPPDVLRLCWACHEPDERARKVSGKLSEKSVHNLKGILSSVFALAV
                     FEGVMDRNPCAQIPRGKLPRIGKQKRPLYDRDEAWALMTDPRVPPDRRVFHAVQGLTG
                     LRVGEVAGLRWQDYDAGAKPLGALHVHCQYDGQPLKTASGADSKARMVPVHPELAKVL
                     SKWRSQGFSAVYGRLPTEEDFIVPDRRNMRARTHSQVTKAPDLDCAAVEIEDKGTHGF
                     RRYFITYARADGASADVLERITHNRRGEIIDVYTSFEWPTLCRAVRCLQVNLDRGTLL
                     DFRPASDRLWDTSLDTDAHRGRKLNDSNPKVVEAVGIENCGFRYESGKYGESQETGSR
                     ATGIKTRGKAVSGNRFAKWQNRSRDDAGKGGRGRCAHRRDLGQLGTPGSFELTAREVA
                     "
     gene            complement(2593..2871)
                     /locus_tag="VFG22_01695"
     CDS             complement(2593..2871)
                     /locus_tag="VFG22_01695"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6414979.1"
                     /translation="MKLGNDDRLIDLTVSDLRAVLLDAVRDVMEEREPGTTSELLTTE
                     ELAKVIKVSPDTARNWRREGCPHVAVGEKKLRWRLADVLRWREELGRG"
     gene            complement(2868..3323)
                     /locus_tag="VFG22_01700"
     CDS             complement(2868..3323)
                     /locus_tag="VFG22_01700"
                     /inference="COORDINATES: protein motif:HMM:NF036299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraK family protein"
                     /protein_id="HET6414980.1"
                     /translation="MDERDRSDLTGRLRKAAEKMPYPYFLLRLEDIEAWLKAGYSVKD
                     VWRIYCEKSVPFPGSYRSFLRYCRKHCESAPAARRRASKAPKATPGRAVSVIGQGKRY
                     PPPRTRPPGLTSEEIQAMMDPDRDLDPERPRIPALVRKADEDHSQWVPR"
     gene            complement(3989..5890)
                     /locus_tag="VFG22_01705"
     CDS             complement(3989..5890)
                     /locus_tag="VFG22_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414981.1"
                     /translation="MAGASVGFGCASQNASITEPCAPHVVDDFEIAYETREANVEVTW
                     YDEWVSRFGKTLPEPLPPAPHWQHPILSGRYAGTMHEDSLSTDVSAFAGPTPNEARVE
                     YVNILEKGERLTGMAPLYEFLDENTVVTIAFGRDAATLLVIDVSGAPKILDQVDLPGR
                     GYGLLDLASSSSRKAAFRDTSGGAYSYLDRDGNVHVPGHDNTVIQIPIRNRRIVREEM
                     THVNLIHEMEEGSIVHHHAARHQDEKINRLTAIMPDADGRIWFTSKYGIVGVFDPGHA
                     ASGCPRVYATSIFFFAIKAKIRELFQPLPPSAEKVLAHLEQAKTEQHIEDVSEVMDEF
                     RELFLGPDSEFTEEIQNSFSVGPDGVYVVTNIALYKLRFNEEARRIELDPKWAPTYAQ
                     GDLIYDNDHTVKPGHLNAGSGTTPTLVLDDHVVIVDNAPSQVNLLAFRQSDGSLVSKI
                     PLFQAGAAAVENSVVAYGHHLIVGNTYGYTDPFRENPTAGGIARFDFDEEKGTYVPVE
                     GWPATGHLDAKTATPKLSLSNGLIYIYNRDTDAEGYSDWQLTALDFRTGYRVFRIKGY
                     FEEGAFRDDLSRIVKKKSLGKLFYDRKVFNNIWATFTFGPRNSIYIGAYRGFVRFSSQ
                     EGVAATEAP"
     gene            5901..6026
                     /locus_tag="VFG22_01710"
     CDS             5901..6026
                     /locus_tag="VFG22_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414982.1"
                     /translation="MKDQKKTGLTRQVCFGDPSTARADQATGGGGAAPCASHFER"
     gene            6053..6589
                     /locus_tag="VFG22_01715"
     CDS             6053..6589
                     /locus_tag="VFG22_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HET6414983.1"
                     /translation="MRFLGLMLLGAGLVLAVNTASAKKENEELSQQVQQIKKDWQEKD
                     DTFNATLEKAYGYVIFPEVGKGGFIVGAAHGTGEVYEKGKLIGHAKLTQTTVGAQVGG
                     QTYAEVILFKNKKALDRFKNSRFEGAAGATAIGGKKGAAATAKYKNGVAIMVLPLKGA
                     MAEAAGGGQKFKFEPLGQ"
     gene            6728..7810
                     /locus_tag="VFG22_01720"
     CDS             6728..7810
                     /locus_tag="VFG22_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414984.1"
                     /translation="MPTFRLKGTIAAMNAGKWAVLAVGIVALLFVWLLFREGLFDSRQ
                     ALDDRAASLDAGVLEQWPFVDGGIEDAGTPDAGVSPAEQPAGPSLGLSGTARLALDHF
                     GAVDKQKRHVLRVKHLNVTMDMDAMRHGAIHVPHGRIEGAEITLYRDKTGKMTIASAF
                     RPGAAPPPSEPSDEETEELGWAIQVGPMILKDVILTLGFTANPVKFRVDHGTMHVRKG
                     GEDSGSMIYFDHIEGAMLEPSPLPHPVRIAHAKGVVRLKGHPMVDMVARTCLGTSELR
                     LRAVVPTRKKPVELTGDSAGAGGLLGRMALLITSKAKAEKVHYTHGAVKLEGGPGCHE
                     APTHAPQPEDGGVQPPAVNDAGVSPP"
     gene            complement(7815..8579)
                     /locus_tag="VFG22_01725"
     CDS             complement(7815..8579)
                     /locus_tag="VFG22_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414985.1"
                     /translation="MSVKKALAVLPVLAVVVLAAWYFGLDFLVRHGIETVGPSLTGTT
                     VEVEDVDLSVFDGRGSIRGLVIGNPRGFQSKYAFKLHQVRIQLVPRSLFGDKIVIREI
                     YVDSPDINYEKKDDTSNLERLVKNIQASVGGGTGGDTDLGDLADQLTGSRKLQIDHFV
                     LKDAKVRFHDQSLNRQGLGIPPLSLELHGLGTRPGGATVSEVSAKVMKKVSTRAGLAV
                     TAQMLTLGMAQDGGEGRRRVSESPDGAHGDEEPHEE"
     gene            8688..10052
                     /locus_tag="VFG22_01730"
     CDS             8688..10052
                     /locus_tag="VFG22_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414986.1"
                     /translation="MMRLRICSLGLMVGLWLLPIPGRAQSDGVLVGDDASLLAGAVTA
                     IVNDGSALYYNPAGLQLVDRNTVDLTITAYSLRLYRIPDALVSTSGVSADANVTEVVI
                     IPAAVSYVRTTKSGLRIGFGVFATSLADYSQRANLTFADAATGFDWDWLIQLSNEISV
                     YHGIAGLGWSTRSRRLHFGATLDLSYVGFSQSSQVGGGLIANQDTGEAVLAASASSSL
                     SLTGLGLRLGFGLLWNPTPAVSFGAAFQTASYLVFDDVSLQSVETAGTAEPLDSAIIL
                     NTLDVSGSDLGFERFEPYRWRLGLAYDFGKVLLSLDVDVQLDEAVSNSTWYATVNGRV
                     GVLVVVSETLGIGFGGFTDRGTTPDEPVSFGEFIVDYYGFTAGLRLNKTRLLAASEEV
                     PSITFESTFSIRYAIGLGTFAGAEVIDDLSTIDPDAPVLAQPNLVDLRIHEISFYVGG
                     GVRF"
     gene            10625..>10900
                     /locus_tag="VFG22_01735"
     CDS             10625..>10900
                     /locus_tag="VFG22_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HET6414987.1"
                     /translation="MSSIATTRLSSRGQIVIPEEVGNELDLEPGAQFVVMGEGDVAIL
                     KKIEAPDRREFQALARKVRSQARKAGAKRSDIKKGHALSSPWSPPRRD"
BASE COUNT         1986 a         3380 c         3366 g         2168 t
ORIGIN      
        1 cggacgagcc acgacaccga gacggctgta gacgtcgtgc acatcatccc aggtccgtag
       61 ctcgcttgac ctgagagcag gccggaggtt ttcccgggcc caacgcgcga agctgtggat
      121 gccctggtgg gcttcgcaat cgatggctcg ctcgggaatc ttctctcggt cgcgcgtctt
      181 cgaccgaagc ggtatcaggc cgagctcccg ctcgagggcc cgggccccgg tgaagagctt
      241 ctggtggtcc caagcgggcg aatgaatccg aaaggtctcc gggtggatct tgttgatggc
      301 aacgtggatg tgctcgtgat ccgtatcgtt gtgacgcaca gcgacgtgct gatgctcgga
      361 aaaccccaga gtatccacaa gcctgtccac aacccgttgg agctcccctc gctcgaggcg
      421 ccgatcgtcg gggtgaagtg agatgacgag atggtaggtg cgcttgctcc cggcgcgggt
      481 gttctgtgat tggacagcgt cgacgagcgc acacgcaagc tcgaggtcgt ctcttccgcc
      541 gacccctggc agattcgcgc tgtagaacca ggtggtgcgc ggcttggcgc ctcgcccctg
      601 aatgtatcgc gcaagcttac ggaagcctcg attcgaagcc gattcgcgct ggtgccgcac
      661 gatcaccgtt gccccgccaa gcggctcacc gcccggcgaa cgttcatgac cgagtcgatg
      721 agctcgaggt tcaggcggcg gtgactcgat gggtccgaaa cgccctcacg aatcgcaagc
      781 ttgagcagcc cgccgatgcg gttgagattg gcgtgaaggc gtgacacttc gctgagggct
      841 tgggcgtcca gagtgctctc gaggcgtgca ccacggagac accggcgaag cacttcgcca
      901 cgggagcagc tcgtctgtcg agcaagagca tcgagacgag cgaagtcgtc ttcatcgacg
      961 cgaaacgaca cgatgcggga tttggccttc ttcgccgcga catgcgcgcc cggaacagca
     1021 gtcacttggc cctcgtcaga taggcgagga gctccaagac agcgtgcgcc agctcacgcg
     1081 acttcgcgtg ccgtgagctc gaaagaacct ggcgtgccaa gctgcccaag gtctcggcgg
     1141 tgggcacatc tcccacgtcc gcccttccct gcgtcgtcgc ggcttcgatt ttgccacttg
     1201 gcaaaccggt ttcccgaaac tgcttttcct cgtgttttga ttccggtggc tcgggatccg
     1261 gtttcctgtg attccccgta cttgcctgat tcgtagcgga atccgcagtt ttcgattccc
     1321 accgcctcca ccacttttgg attggaatca ttcagctttc ggccgcgatg tgcgtcggtg
     1381 tccaaagaag tgtcccaaag ccggtcgctc gcgggccgga agtcgaggag cgtcccgcgg
     1441 tcgaggttga cctggagaca gcgcaccgcg cggcacaagg tcggccactc aaagctcgtg
     1501 tagacgtcga tgatctcgcc gcgccgatta tgggtgatgc gctcgaggac gtcggcgctt
     1561 gcgccgtccg cccgcgcata agtgatgaag tagcgccgaa agccgtgagt gcctttgtct
     1621 tcgatctcga ccgcagcgca gtcgaggtcg ggcgccttgg tgacctgcga gtgcgtcctg
     1681 gcgcgcatat tccggcggtc aggcacgatg aagtcctcct cggtcggcag ccgcccgtag
     1741 accgctgaaa agccttgtga ccgccactta gaaagcacct tggcgagctc gggatgcacc
     1801 gggaccatgc gcgccttgct atcggccccg ctcgctgtct tgagcggctg gccgtcatac
     1861 tggcaatgca cgtgaagcgc gccgagcggc ttcgcgccag cgtcgtagtc ctgccatcga
     1921 aggccggcca cctcccctac ccgaagaccc gtgagcccct gaaccgcgtg gaacacccgg
     1981 cggtcgggcg ggacgcgcgg gtcggtcatc agcgcccagg cttcatcgcg atcgtagagc
     2041 gggcgcttct gcttcccgat cctcgggagc ttgccacggg ggatctgagc gcaaggattg
     2101 cgatccatca ccccttcaaa gaccgcgagc gcaaacacgc tcgaaaggat gcccttgaga
     2161 ttgtgaacgg acttttcgct gagcttgccg ctcacctttc gtgcacgctc atcgggctcg
     2221 tggcacgccc aacaaagtcg gaggacatcg ggcggtgtga tgctggcgat gggacgccgc
     2281 cccagcgtcg gaagcacgtg caagcgaagc tggcgcacct cacggtcgag gttgcgccgc
     2341 ctcccgacgg cctgctgggc ctcccaacgc ttcacgaact cagcgagcgt cacattcggc
     2401 ccgacgcccc gctcgaggtc gagcgtccct tcacgcacct gtcgttttcg cgtgctgagt
     2461 ttgcgcttag ccgcttcgaa gcttcgtcca gcatgctcgc ggcgccatct tccttcaccg
     2521 tccttgtagc tgatgcggta gcgctgcttg gcttcatccc aacggatccc ggttgtcgat
     2581 cgcttctttt gcttagccac gcccgagctc ctctcgccag cgcagcacat cggcgagccg
     2641 ccaccgaagc ttcttttcac cgacggcgac atgtggacac ccttctcgtc gccagttgcg
     2701 cgccgtgtcg ggcgagacct tgatgacctt cgcgagttcc tcggtggtca gaagctcgct
     2761 cgtcgtccca ggctctcgct cttccatcac gtcgcgcact gcgtccagca acaccgcgcg
     2821 cagatccgac acggtcagat cgatgagacg atcgtcgtta ccgagcttca tcttggtacc
     2881 cactgagaat gatcttcatc ggcttttctt acaagagccg ggatacgcgg tcgctcgggg
     2941 tccaggtcgc ggtcagggtc catcatcgcc tggatttctt ctgacgtgag cccaggaggg
     3001 cgtgtgcgtg gaggcggata tcgcttgccc tggccgatta cgctgacggc tctgcctggc
     3061 gtggccttgg gcgctttcga ggccctgcgc ctagcagccg gagcgctctc gcagtgcttg
     3121 cggcagtagc gaagaaacga ccgatacgag cctggaaacg gaacgctctt ttcgcagtag
     3181 attcgccaca cgtccttcac ggagtagccc gccttcaacc acgcctcgat gtcttcgagc
     3241 cgcagaagga agtacggata cggcattttc tcggcagcct tgcgtagccg cccggtcaaa
     3301 tcggatctgt cgcgctcgtc catcactcgt cttatcggtt ttgccccccg aaaagttgct
     3361 ctggcggagc atcttttttc gcatgcgaag aatcaacggc aattccgacg gcctgaggcc
     3421 ggctttcgcg tcctcggcgc gacttggcgc tacctgtcgc tatttggcgc tcgcagtgca
     3481 ctcgctgtca ctcgcgcgac actgttcggc gtacgcgcga caccatctgg cgcacgcgcg
     3541 acacttcctg tcacttcttg tcattcttgc caggaagtgt cgcggttgtg actgagagcg
     3601 ccaggttgtg actcacccgg gacaaccagc gtcagtttgt gactcgctac cggagggcaa
     3661 ggcatagttt tggcagacac caaaactgca cgctccctcg ctcgctcggt tgcacgaact
     3721 acgcgctcaa cgccttcggc ttattcgctc gatcgtgcta gccgtctttc cgggcgagtg
     3781 tcgacgaaag cgtcaagctc ctgacggtgt ttcgcgcttc ccgactcttc ccgtcgccgc
     3841 tcgaagattg gtctcgggac cggggggcgc cccattccgg aaacccccaa tgaatcaggg
     3901 accctcgagc gcctacgcat caaggtacgc ccgaagacga cgaggatcgt gcgcgacacg
     3961 ggggcttcgg tgagaagcga ttgaccggct acggtgcttc ggtcgcggcg acaccttcct
     4021 gcgaggagaa gcggacgaac cctcggtacg ctccgatgta gatcgagttg cgtggtccga
     4081 aagtgaaggt ggcccagatg ttgttgaaca cctttcggtc gtagaagagc ttgccgagcg
     4141 atttcttttt cacgatgcgc gagaggtcgt cgcggaaggc tccctcttcg aaatagcctt
     4201 tgatgcggaa gacccgatag cccgtacgaa aatcgagtgc ggtgagctgc cagtcgctgt
     4261 agccctcggc atcggtgtct cggttgtaga tatagatgag gccgttcgac agcgagagct
     4321 tcggcgttgc ggtcttggcg tcgagatgac ccgtcgcggg ccagccctcg acgggaacgt
     4381 aggtgccctt ttcctcgtcg aagtcgaatc gtgcaatgcc gcccgcggtg ggattctcgc
     4441 ggaacggatc ggtgtatccg taggtgttcc ctacgatgag gtgatgaccg taagcgacca
     4501 ccgagttttc gaccgcggca gcgcccgctt ggaacaacgg gatcttgctg accaaggatc
     4561 cgtcgctttg acgaaacgca agcagattga cttgactagg cgcgttgtcg acgatgacca
     4621 cgtggtcgtc gagcaccaag gtcggcgtcg ttccgctccc cgcgttcaag tggccgggct
     4681 tgaccgtgtg gtcgttgtcg tagatcaggt ctccttgtgc ataggtcggc gcccacttcg
     4741 ggtcgagctc gatgcgtctc gcctcttcgt tgaagcgcag cttgtaaagg gcgatgttcg
     4801 tcacgacgta gacaccgtcg gggcccacgg agaaggagtt ctggatctcc tcggtgaatt
     4861 ccgagtccgg gcccaagaag agctcgcgga actcgtccat aacctcggac acatcctcga
     4921 tgtgttgctc ggttttggcc tgttccaagt gcgcgagtac cttctccgca ctagggggca
     4981 gaggctgaaa cagctctcga atcttcgcct tgatggcgaa gaaaaagatc gaggtggcgt
     5041 acactcgggg gcaaccgctt gccgcgtgtc cgggatcgaa cacacccacg attccgtact
     5101 tcgaggtgaa ccaaatgcgt ccgtccgcat ccggcatgat cgcggtgagc ctattgattt
     5161 tttcatcttg gtgccgcgcc gcgtggtgat gaacgatgct tccctcttcc atctcgtgga
     5221 tgagattcac gtgggtcatc tcttctcgta cgatgcgccg gttgcggatc ggaatttgga
     5281 tgaccgtgtt gtcgtgaccc ggcacgtgca cgttgccatc gcggtcgagg tacgagtagg
     5341 cgcctcccga agtgtcgcgg aaggctgcct ttctcgagct cgagctagca agatcgagca
     5401 ggccgtatcc tcggcccggg agatccacct ggtcgagaat ctttggagct cctgagacgt
     5461 cgatgacgag aagggtcgca gcatcgcgcc caaatgcgat cgtgacgacg gtgttctcgt
     5521 ccaggaactc gtagagcggc gccatgccgg tcaatcgctc gcccttttcg aggatgttca
     5581 cgtactcgac acgcgcctcg tttggcgtcg gtccagcgaa ggcgctcacg tcggtcgaca
     5641 ggctgtcctc gtgcatggtg ccggcgtatc gacctgagag gatcgggtgc tgccagtgag
     5701 gcgctggcgg aaggggctcc ggcaaggtct tgccaaaccg ggacacccat tcgtcgtacc
     5761 aggtcacctc gacgtttgct tctctggtct cgtaggcgat ctcgaagtcg tcgacgacgt
     5821 gtggtgcaca gggctcggtg atgctcgcgt tttgcgaagc gcagccgaac ccaacgctcg
     5881 cacctgccat accgagcaag atgaaggacc aaaagaagac gggtctcacg cgccaagtgt
     5941 gtttcggcga cccatccacg gcaagggcgg atcaggccac cggtggtggc ggcgccgcgc
     6001 cttgcgcgtc gcacttcgag cgctaagcag cagcccaaag gaggattgtt tcatgagatt
     6061 cttgggtttg atgttgttgg gtgctggttt ggtgctcgcg gtcaacacgg cttcggccaa
     6121 gaaggagaac gaggagctca gccagcaagt ccagcagatc aagaaggact ggcaggagaa
     6181 ggacgacacc ttcaacgcca cgctcgaaaa ggcctatggt tacgtgatct ttccagaggt
     6241 tggaaagggg ggcttcatcg tgggcgccgc ccacggtacg ggcgaagtct acgaaaaggg
     6301 caagctcatc ggccacgcca agctcacgca gaccaccgtc ggtgcgcagg tggggggcca
     6361 gacctatgcc gaggtgattc tattcaagaa caagaaggcg ctcgaccgct tcaagaacag
     6421 ccgcttcgag ggagccgcgg gagccacggc gatcggcggc aagaagggcg cggcggccac
     6481 cgccaagtac aaaaacggcg tggccatcat ggtcttgccg ctcaagggcg ccatggccga
     6541 agcggcgggc ggcggccaga agttcaagtt cgagccgctt gggcaatagc ccctaggtcg
     6601 catccgccgc ggagaagtgg cgaccctggc tggacgctgt gctcgtgtcc tagacagggt
     6661 cgtcgtttcc gactcctgag ttgttcctcc gcctagcgac gtcagccgga ggctcgcagt
     6721 ttccgtattg ccaacatttc ggctcaaggg caccatcgcg gcgatgaacg cgggcaaatg
     6781 ggcggtgttg gcggtcggta ttgtcgctct cctttttgtc tggcttctat tcagagaagg
     6841 ccttttcgac tccaggcagg cactcgatga tcgagcggca tccctcgatg ccggtgtgct
     6901 cgaacagtgg ccgttcgtcg acggcggcat cgaggacgca ggaacgccgg acgcgggtgt
     6961 gtcccccgca gagcaaccgg caggcccttc actcggactc tccggcacgg cgcggcttgc
     7021 gctggaccat ttcggagccg tcgacaagca aaagcgccat gtgctccgcg tgaagcacct
     7081 caacgtgacg atggacatgg atgccatgcg gcacggagcg atccatgttc cccatggacg
     7141 catcgaaggc gccgagatca cgctctaccg cgacaagacg ggcaagatga cgatcgcgag
     7201 tgcctttcgc ccgggtgcgg cgccaccgcc atccgagcca tccgacgaag agaccgaaga
     7261 gctaggatgg gccattcaag tgggccccat gatcctcaaa gacgtgattc tcacgctcgg
     7321 cttcaccgcg aaccccgtga agttccgggt ggaccacggc acgatgcatg tccgcaaagg
     7381 aggagaggac tccggatcca tgatctactt cgatcacatc gagggcgcga tgctcgagcc
     7441 gagcccgctt ccgcacccgg tgcgtatcgc gcacgccaag ggcgtcgtgc gcttgaaagg
     7501 tcaccccatg gtcgacatgg tcgccagaac ttgcctaggc accagcgagc tgcggctgcg
     7561 cgccgtcgtc cccacccgaa agaagcccgt cgagctcacc ggcgattcgg ccggcgccgg
     7621 gggcctgctc ggacgcatgg ctctgttgat cacgagtaag gccaaggccg agaaggtcca
     7681 ctacacgcac ggcgccgtga agctggaagg cgggcccgga tgccacgagg ctcccactca
     7741 cgccccccaa ccggaagacg gcggtgttca gccgcccgca gtgaacgacg ccggcgtttc
     7801 tcccccgtga gcgcttactc ctcgtgcggt tcttcgtccc cgtgcgcgcc atcgggggat
     7861 tcggacactc gccttcggcc ttctccgccg tcttgggcca tgccgagggt cagcatttgc
     7921 gcggtgaccg caagccccgc acgcgtcgag actttcttca tgaccttcgc gctcacctcg
     7981 gagaccgtcg caccaccggg gcgggtccca aggccgtgga gctcgagact cagaggcggg
     8041 atccccagcc cctgacgatt taggctctgg tcgtggaaac ggaccttggc atccttcaac
     8101 acgaagtggt cgatctgcag ctttcgtgat cccgtcagtt gatcggcgag gtcgccgaga
     8161 tccgtgtcgc ctccggtgcc gcctccgacg ctggcctgga tgttcttcac gagtcgctcc
     8221 aggttgctcg tatcgtcttt cttctcgtag ttgatgtcgg gcgagtcgac gtagatctct
     8281 cgaatcacga tcttgtcgcc gaaaagcgac cgagggacga gctgtattcg cacctggtga
     8341 agcttgaacg cgtacttgct ttggaagcca cgagggttcc caatcaccag acccctgatc
     8401 gatccgcggc cgtcgaagac cgagagatcc acgtcctcga cctcgacggt cgtccctgtg
     8461 agtgagggtc cgacggtctc gatgccgtgc ctcacgagaa agtcgaggcc gaagtaccag
     8521 gccgcgagaa ccactacggc gagaaccgga agcacggcca gcgccttctt gacactcatc
     8581 ggatacagat tgcacttttc gggggctttc gccatggggg cgtgagaccc cggccggcgg
     8641 tcgattgagt ttcacccgag acttgtgtcc aagtacgata gccgcggatg atgcgcctgc
     8701 gtatctgttc tctcgggttg atggtcggcc tttggctcct gccaattccc ggaagggcgc
     8761 agagcgacgg cgtgttggtc ggggacgacg cttccttgct cgcaggcgcg gtgacggcga
     8821 tcgtcaacga cggaagcgcg ctctattaca accccgcagg cctccaactg gtcgatcgaa
     8881 acacggtaga cctgacgatc acggcataca gcttgcgtct ctaccgaatc cccgatgccc
     8941 tggtctcgac ttccggggta tcggccgacg cgaacgtcac cgaagtcgtg atcatcccgg
     9001 cggctgtgtc gtacgtgcgc acgaccaaga gcggcttacg catcggattt ggcgtcttcg
     9061 ccacgtcgct cgcggactac agccagcgag ccaatctcac gtttgcagat gcggccactg
     9121 gattcgactg ggactggctc atccaactga gcaacgagat ctcggtctat cacggcatcg
     9181 ctggccttgg ttggagcacc cgaagccggc ggcttcattt cggtgcaacc ctcgaccttt
     9241 cgtatgtcgg cttctcgcaa tcgtcccagg tcggcggcgg gctgatcgcc aaccaggaca
     9301 ccggcgaggc tgtcctcgcc gcctcggctt cgtcgagtct gtcactcacg ggactcggac
     9361 ttcgactcgg gttcggcctt ctctggaacc cgacgccggc cgtcagcttc ggagcggcct
     9421 ttcaaaccgc gagttacctg gtcttcgatg acgtgtctct ccagagcgtt gagacagcgg
     9481 ggacggctga accgttggat tcggcaatca ttctcaatac cctcgatgtg agcggctccg
     9541 atcttgggtt cgagcggttc gagccctatc gatggcgcct agggcttgcg tacgatttcg
     9601 gaaaggtgct cctgtcgctc gacgtagacg ttcagctcga tgaagccgtc tccaacagca
     9661 cctggtacgc taccgtcaac ggcagagtgg gagttctggt ggtcgtaagc gaaacccttg
     9721 gaatcgggtt cgggggattc accgaccgag gaaccactcc cgacgaaccc gtgagcttcg
     9781 gcgagttcat cgtagactac tacggcttca ctgcagggct caggctcaac aagacacgat
     9841 tgctcgccgc gagcgaagaa gttcccagca tcacgttcga gtcgacattt tcgattcgct
     9901 acgcgatcgg gttggggacg ttcgctggcg cagaggtcat cgacgacctc tcaacaatcg
     9961 atcccgatgc gccagtgctg gcacagccca atctggtgga tctccgcatc cacgagatca
    10021 gcttctacgt gggcggaggc gttcggttct agagcctcga agtctcagca agctaccgat
    10081 cctggacgct gcacgacggc gacaggctcg accgcatcga cctgagggtg cttggcgttc
    10141 gcaaacttgg gacccacgcg agagagtgaa gcgcgagaac gcggaaagta gcctcgaccc
    10201 cctgctcgct tactcaaatc acacgcagca ttccccacgc gaggcgtgtg cgcgccagca
    10261 cggcgtcgag tgagtccagg ttttggacac gtgtccgcag tggatccgcc aacgcgcatt
    10321 caccgggttt caacagcgtc tcctcgtcga cacccttccg cgtaatcgct actgaatcgc
    10381 gcgaaccagg gcgttctggg gacggttcga ttcccaccgc ctccaccaaa caagccaagt
    10441 gattgcttgg ggttagcgaa ttcgacgatc gcgcgagtga ccgcatagag accgtcatcg
    10501 gctgctcttt gcatccgttc ggtggatttc gccaacgttt cccgggtacc ggaattggcg
    10561 tagggcgtcc ttacccaagt ccatccggcc ttctcttacc agtaagaacc gcctgggacc
    10621 actcatgagc agtatcgcga ccacaagact ctcgtccagg ggccaaatcg tgattcccga
    10681 ggaggttggt aacgagctcg atctcgaacc gggggcccag ttcgttgtca tgggcgaggg
    10741 cgacgtcgca atcctgaaga agatcgaggc accggacagg cgagagttcc aggcgctcgc
    10801 tcgcaaggtt cgaagtcagg cccgcaaggc cggagcgaag cgctccgata tcaagaaagg
    10861 gcatgcgttg tcgagcccat ggtcgccgcc cagacgcgac
//
LOCUS       DASRZF010000039        14734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3121463, whole genome shotgun sequence.
ACCESSION   DASRZF010000039 DASRZF010000000
VERSION     DASRZF010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 14734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14734
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3121463"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VFG22_01740"
     CDS             complement(<1..518)
                     /locus_tag="VFG22_01740"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET6414988.1"
                     /translation="MKRTDTIIVGGGQAGLAMSRCLSEEGIEHVVLERGRVAERWRTE
                     RWDSLRLLSPNWHTRLPGFRYQGADPDGFMTMPEIVSYFERYALASGAPVRDETTVLS
                     VKRCGQGYLVRTDQGDWEAPTVVVATGHCDMPFVPQVAEGLSSGVHQVVPSRYRNPEQ
                     LPDGGVLVVGASA"
     gene            complement(602..1210)
                     /locus_tag="VFG22_01745"
     CDS             complement(602..1210)
                     /locus_tag="VFG22_01745"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET6414989.1"
                     /translation="MESNATPSAQTSNPKPRREKRAMNGVDVPTLLATINAVGQQPEL
                     AKFQFRASNQWVKGTHSRTRIEGFRGAGGEHEQTADFVYDADHPPVLCGKGNAPTPVE
                     FLLHAIATCITAGIGNIASARGIELESVECSVEGDLDVQGLLGLSDEVRNGYEAIRLN
                     FKVKGDADAETLRKVVAQSQARSAVYDVLTNGVPVSIEVDAA"
     gene            complement(1350..1598)
                     /locus_tag="VFG22_01750"
     CDS             complement(1350..1598)
                     /locus_tag="VFG22_01750"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-binding protein"
                     /protein_id="HET6414990.1"
                     /translation="MEYVVLEREFSEPLTPDEVRKMAAETQCLDLYRVKPVRSYLMPG
                     GQRMICVFLAPDAEALRAVSRSNGFENAVIWPSTVHTP"
     gene            1649..4783
                     /locus_tag="VFG22_01755"
     CDS             1649..4783
                     /locus_tag="VFG22_01755"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6414991.1"
                     /translation="MTPDSIAGSLLGVSVAALQVQVLGPLSVLRDGEPVRLPQSKKTR
                     GLLAYLAITGRAHRRDRLCSLLWDVADDPRGALRWSLSKIRPVVDTDVTRCLIADRES
                     VELKLEKARLDWRWVRHELASGLDSLSTERLKHLESAFEGELLEGLDLTGFDEFTAWC
                     AAEREHARKLHVEVLRALVARFESTAIDALPYSRELVRINPVDEDARADLIRLLVRAG
                     RRNEAKAQYESAARMLKELGAKVMGPLETAWREVNRRDDAPSDEHFPSPVETNGAATS
                     TDASASPSPRSRDVFVGREKERHRLLSLLDDASTGGLTVFMLKGESGIGKTRLLAELS
                     AEARRRGSTIFEATAYEAEAGRPYGPWIDALRRLPADALTHADGNALAPLLPELGGSA
                     AEAGASRDRLFGAVLDLVTALARREAPAVLLFDDIHWCDDASAALLHYVARMSLEHPV
                     LVVLGARDGELIDNESVIKMLRSFRREGLIDEHLLLPFTERDVEALIRSIDGSADATE
                     VFNLSGGNALMARELAHASSAGETVPGSLKELISDRVERVAPEAGDVLRWSAVLGTAF
                     DVSRLSELVSLDFDRLMSALRELERHALLESSGSSANMYAFHHALVHRVVYSEISEPR
                     RKLMHLRVARLLAEAKDPEGVISLELAHHASVGGDAAMAVRACVTAGARCLRVFANAE
                     AYTHARRGLRLVEQLEDPERAKLRIELEQISLGAHWPEDPDAESTRIEALAEQALDAG
                     CPEHARLAFTLVSTLRWHEGSLSDAHRLSLRAELASRGTTDQHRAIGIAETARCLVIL
                     ERDLAQAEALLLEARALMDRSGEPTWAVFDGVGLLRLHAGALDDAKTAFEEAWMLAKK
                     SGNRLQEFMALEHLVMTSIHRRDPDEACQYAKELAVIGEKLREGSERPLARAMLALCR
                     YNRGEVEDRSAFEEALQELRDVDAKQRLAFVLTQAAAVEAERGNLQSAATRAAEAEKL
                     GQILQQPSESGLASAVLLRIARERGDRAAMHERMQELRAISGYAKHVGEIVERELATA
                     AALSGTELRDMGAE"
     gene            4783..5055
                     /locus_tag="VFG22_01760"
     CDS             4783..5055
                     /locus_tag="VFG22_01760"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HET6414992.1"
                     /translation="MARVMVERKFDDAQSFDDLQAQEVSFAWCAEQHQVRFIRSYFSR
                     DRKLMICEYDAPDAESVRTLQRTARMPFERIWTAMMFDWEADSDLE"
     gene            5048..6373
                     /locus_tag="VFG22_01765"
     CDS             5048..6373
                     /locus_tag="VFG22_01765"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HET6414993.1"
                     /translation="MSDRKERLRRELRQRLLADRQDLPVSALARFSRTGSTGLRIGRA
                     MLAAGRSGSDGEVDIETLVKIVGSIAQLKGITMKMGQIMSYIDVALPDDLRDALSVLQ
                     THAQPMSLEQVQDILRQELGAQANGLLDNMEAAPVAAASIGQVHRSRLPSGERVAVKV
                     QYPQVEKAIESELKPAAIGTAISAIVYPGAKIDDFIAEARARFLEECDYIHEAAAQDR
                     FAQIYRGHPHIAVPSVHEAYSSRRVLTSTWLEGIGFDAFLAGAPTQSMRDRIGEALFG
                     FYVGALFEHALYNCDPHPGNYLILDDGRVGMLDYGCTREFDRSFVVQLARLTRAVHSD
                     EHALLHRAFLDLGMVRDGQSYDFDTARDLVRSFYGPMLRDAVQSIDLGEAIGMRRVFA
                     RKRQLLKLRLPGEFLFLFRIRFGLMSVLQRLGAQANWYNLERQCIAAIA"
     gene            6441..8090
                     /locus_tag="VFG22_01770"
     CDS             6441..8090
                     /locus_tag="VFG22_01770"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6414994.1"
                     /translation="MRNSDFISTFGLAMTQSGLTCRLLGPLRIERNGKAIELPRSKKT
                     RALLGYLAARGGEHRRQRLTDLFWDVADDPRGGLRWSLSRLRGLVDEPGRARVVADRD
                     CVRLDLSDADVDLHRVQAAKTGLGTLDTDTVGELAALFQGELLEGIDLPEFTEFQAWL
                     TAVRGDCRQLRIQVLDEFVGRSSDDLERALPCARDLVRLDSLNVQRWLHLLDLLERTG
                     RGREAEQQIEVGRRTLTEQGIDDGALVEAKRVMLGNPRARLKSPRAQELRQEIRFCTS
                     FDGTRIAYATVGEGRPILKAANWLTHLEFDWESPVWRHVFTELSRNNLLVRYDDRANG
                     LSDWDVEDISFDAFYKDLEGVADALKLDRFALLGISKGASVSAAYAARHPDRVSHLIL
                     LGGFATGRLVEASERSREHEMAMRTLMRVSWGADNPAFRHLFTSTFIPNATPEHVKWF
                     NDLQRISASPDNALRLRIAAADIDVRSLLPKIQAPTLVMHSRGDSAVEYGRGLALAGG
                     IPNARFVTLESDNHLLTEDEPAWTKFLDEVRRFLGESENGA"
     gene            8121..8762
                     /locus_tag="VFG22_01775"
     CDS             8121..8762
                     /locus_tag="VFG22_01775"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HET6414995.1"
                     /translation="MQNQVLHVLTVFMGFFAIMNPIANTPIFLGLTEGDSADIRRAVA
                     RRALLVTFLIIVVFTISGRMIFELFGIGLPAFQITGGLLVLLIGFHMVQGHASRVQHP
                     GNAGATPGLEGELDKAISPLAMPLLAGPGTISTAINFASQGGIGPVVTTLVTFFALCV
                     ITYFFFVYGERLVRYLGHAGMSIITRLMGLILAVIGTQMVIGGITGAIKLAAS"
     gene            8786..10264
                     /locus_tag="VFG22_01780"
     CDS             8786..10264
                     /locus_tag="VFG22_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414996.1"
                     /translation="MNPRIVSLGAIWTTCLPLSVVLLSCVSQTPAAKPAQATSPEFGM
                     AQDIVSFAAAVSKSLNDRSAKPAPSNTYSYETAYGPDELAEPPVYLAPTATYSGLAST
                     DCSGWVSFVVNTVSPLHEAVLQSQGRLPAYNQVYPDGFALEEGQRPWSRAFVLTNFFR
                     AEYAKSSGLAPVPRFEELRSGDIGAYAMGRYVDPSDASLIKTTDTGHTFIVMGPPSIV
                     DPNTADYDGQGTLSAQAVKVIAVPVIDSSAAVHFDPDARKNEQGQYSLPKSSPYADAK
                     PGGVGIGTLWFALGEDGRVLQRRLGPGETYADVVVGAARLQNVISLQPEILDERGDLL
                     VEIFDNSPSEFDGSSFGPAPIDLTGPGGIRLADGRLGLNGRSDFSGGVTVDSGELVVE
                     SDGALGTGDIDVRGGALTLRSPALGDTAALQLSDDLEDGAVRLDFSGTDIVGTFRIGD
                     KVHRCGTWGAVGSGAMFTDTVFSGAGILQLAAEPIESCSLKN"
     gene            10304..10789
                     /locus_tag="VFG22_01785"
     CDS             10304..10789
                     /locus_tag="VFG22_01785"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET6414997.1"
                     /translation="MTDISTSASVLIPRPRDEVFARATDSTNTPETLRARGPFAGITR
                     VEMDEGQVLAKGTKRRVFMTDGTVLEEVILDYDPPVRHRYRWTGGAKFPFSLLVRSGT
                     GNWDFIETEGGTRIVWTYTFGLTSPLAYPLAIPIVLLFKRWLQQGLDAIRAELVAEPE
                     P"
     gene            10840..11199
                     /locus_tag="VFG22_01790"
     CDS             10840..11199
                     /locus_tag="VFG22_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6414998.1"
                     /translation="MSKTRLDLPFVFSALLALSACGEAVTVEDQGGASGTAGAGGGGD
                     TGGRGIGGSAGSENTGGAGGVSYLVCTLGLCMEDEALARSCQEVYDVCVGQGHYTRCC
                     RMDADKTCGVFGETGPY"
     gene            11229..12107
                     /locus_tag="VFG22_01795"
     CDS             11229..12107
                     /locus_tag="VFG22_01795"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HET6414999.1"
                     /translation="MRIAVSTLVVLAAAIAHSGCTVAEDESGEGGSGAGGGGEGGGGG
                     SGGSAGTGGANGNTGTAAVAGPETCDLGAADRLPIADAYITGDGPEADRMLADTNFGG
                     ATTLLCKSAANLGFTRKTYMVFDLSDQSAAVQSATLVLTLERHIEGPTPETSGPQPFN
                     FYGIIDDEDWDPATLPETSTTDGITWNNAPRNKNDWRVPFEDSPGVPLLIAGYDFMLG
                     GDFDQDADGVDDPGTRYAFDLTDYINERIANDADGKVSVLIAHDNQTGVNVDTSVFFS
                     KEYADDECDRPFLRLE"
     gene            12134..12736
                     /locus_tag="VFG22_01800"
     CDS             12134..12736
                     /locus_tag="VFG22_01800"
                     /inference="COORDINATES: protein motif:HMM:NF017697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate hydrolase family protein"
                     /protein_id="HET6415000.1"
                     /translation="MTRGTPKRPFENRSRRYLEELLACRGVEEDLELRSRFGFMAFHG
                     GSLETQTDAIAAGAAQKSGASFYAVRQPAHLRWHIPSSLFRADESDKLQAFFESVDVV
                     VTVHGFRRKAFPHHVMLGGRNRTLAAHLAETLRPELPGAVVVDDLSKIPSSIRGVHPD
                     NPVNVPRLRGVQMELSPLLRTQPENLVEALAQAARTFSPA"
     gene            13050..14513
                     /gene="gabD"
                     /locus_tag="VFG22_01805"
     CDS             13050..14513
                     /gene="gabD"
                     /locus_tag="VFG22_01805"
                     /inference="COORDINATES: protein motif:HMM:NF008415.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HET6415001.1"
                     /translation="MQNLSDTRLFRQRCYVDGDWTQAEGGRSVAVTNPVNGEVIGHVP
                     DCGAAETRKALQAAESALPGWRALPARERGVKLRAWFDLMMANQDDLARIMTIEQGKP
                     LAESRGEIAYAASFIEWFAEEGRRTYGDVIPQHQADKRIIVIKQPIGVCVAITPWNFP
                     AAMITRKAGAALAAGCTMVVKPALETPYSALALCELAERAGIPAGVLNVVTGDAKSIG
                     GEMTSSPIVRKLSFTGSTAVGKLLMEQCAGTVKKVSLELGGNAPFLVFEDADLDAAVE
                     GAIASKFRNAGQTCVCSNRFLVHDQIYDEFARRLANAVREQLKVGNGLDEGVAQGPLI
                     SKAAVDKVERHISDAVSKGAKVAIGGARHALGGTFFEPTVLTDVTPAMAVAQEETFGP
                     LAPLFRFESEEEAVRMANDTEYGLASYLYSRDVSRVWRVAEALEYGMVGINTGLISTS
                     IAPFGGVKSSGIGREGSKYGIEDYLEIKYMCIGGINP"
     gene            14547..>14734
                     /locus_tag="VFG22_01810"
     CDS             14547..>14734
                     /locus_tag="VFG22_01810"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET6415002.1"
                     /translation="MAMATNSELLSRRNQAVARGVASMHPRFAERAENALVWDVEGKR
                     YIDFAGGIGVLNTGHRHP"
BASE COUNT         2808 a         4590 c         4617 g         2719 t
ORIGIN      
        1 gccgaggcgc ccacaacgag cacgccacca tctggaagct gctctgggtt cctgtatcgg
       61 gacggcacga cttgatgcac accggacgaa agaccttccg cgacctgggg gacgaatggc
      121 atgtcgcaat gacccgtcgc cacgacgacg gttggggctt cccagtcacc ctgatcggtt
      181 cgcacgaggt acccctgacc gcatcgcttc acactgagca cggtcgtttc gtcccgcacc
      241 ggggcgccgg acgccagcgc gtagcgctcg aaatacgaga cgatttccgg catcgtcatg
      301 aaaccatccg gatcggcacc ctgatatcgg aaaccgggaa gccgggtgtg ccagttcgga
      361 ctgagcagcc ggagagaatc ccagcgctcc gtgcgccagc gctcggcaac cctgcctcgc
      421 tcgagcacca cgtgctcgat tccttcttca gacagacacc ggctcattgc caagccagct
      481 tgcccgcctc ccacgatgat ggtgtctgtg cgcttcacga tgatctctcc tctctcccgg
      541 cgggatccgc cgaccggccc gcgtcagtca ggccggtcgg catgctccgt cgaccggttc
      601 actacgcggc atccacttcg atcgacactg gaactccatt ggtgaggacg tcgtacaccg
      661 cagagcgtgc ctgcgactgc gcgacaacct tgcgaagggt ctctgcgtcg gcatcgccct
      721 tcaccttgaa gttgaggcga atggcctcgt agccgttgcg aacctcgtcg gagagtccga
      781 gcagaccctg cacgtcgagg tcgccctcga ccgagcactc caccgattcg agctcgatgc
      841 cccgcgcaga ggcgatgttt ccgatgccgg ccgtgataca agtcgcgatc gcgtgcagga
      901 gaaactcgac cggggtcgga gcgttgccct tgccgcaaag gaccggcggg tggtcagcgt
      961 cgtacacgaa gtctgccgtt tgctcgtgct cgccgcctgc gccgcggaag ccctcgatgc
     1021 gggtccggct gtgggtgcct ttcacccact ggttcgacgc ccggaactga aacttcgcca
     1081 gctccggctg ctgaccgacg gcgttgatgg tcgccagcag agtgggaacg tctacgccgt
     1141 tcatcgcccg cttctcacgg cggggcttcg ggttgctggt ctgagctgat ggggttgcgt
     1201 tgctttccat ggtttcctct ttcgtttgag ttgggtggcg gggttcttcc cgcccacgca
     1261 aatcaatcta cggaggcacc gtgtgagcct gcgtgtgaag gaccgtggaa aaagtcgcgg
     1321 tcgcgattct tcgccctaag tcaccgaaat cacggggtat ggaccgtcga cggccagatc
     1381 acggcgtttt cgaatccgtt cgaccggctt acggcgcgta gggcctcggc gtcgggagcc
     1441 aggaagacac agatcatgcg ttgcccgccc ggcatcagat agctgcggac cggcttgacc
     1501 cggtagaggt cgaggcactg tgtctcggcg gccatcttac gaacctcgtc cggggtcagg
     1561 ggctcagaga attcgcgctc taagacgacg tactccatac ctcgatgata ccacggagcc
     1621 atgagccatc agagcccgaa acgatgattt gacgccagat tccatcgcgg gcagccttct
     1681 cggggtgagc gtcgccgcgc tgcaggtcca agttctcgga ccgctctctg tccttcggga
     1741 cggtgagccg gtgcgcctgc ctcagtccaa aaaaacacgt ggcttactgg catacttggc
     1801 catcaccggc cgcgcccacc gccgcgaccg gctctgttcc ctactgtggg atgtcgccga
     1861 cgatccccgc ggcgccttac gctggagcct gtcgaaaatt cgcccggtcg tcgatacaga
     1921 tgtgacgcgc tgcctaattg cagaccgcga gagcgtcgag ctcaaactgg agaaggcaag
     1981 gctcgactgg cgctgggttc gacacgagct cgcatctggc ttggattccc tctcaaccga
     2041 gcgtttgaag caccttgaat ccgcgttcga gggcgagctt ctcgaggggc tcgacctcac
     2101 aggcttcgac gagttcaccg cctggtgcgc agcggagcga gagcacgcgc gcaaactgca
     2161 tgtcgaagtc ttgcgggcgc tggttgcccg attcgagtcc acggcgatcg acgccttgcc
     2221 atactcgaga gagctcgttc gaatcaatcc cgtcgacgag gacgcgagag cggacctgat
     2281 tcggctcctc gtgcgggcag gacgaagaaa cgaagccaag gctcaatatg agagcgcggc
     2341 acgcatgctc aaggagctcg gcgccaaggt catgggcccg ttggagaccg catggcggga
     2401 ggtgaaccgc cgggatgacg ccccaagcga cgagcatttt ccatcgccgg tcgagacgaa
     2461 cggtgccgcg acgtcaactg acgcaagcgc gtccccgagt ccgcgaagtc gggacgtatt
     2521 cgtgggtcgc gagaaggagc ggcaccgcct tctttcgctc ttggatgatg caagcactgg
     2581 aggcttgacg gttttcatgc tcaaagggga gtccggtatc ggaaagacca gactcctcgc
     2641 agagttgagc gcagaagcac gtcgccgagg ctccacgata ttcgaagcca ctgcgtacga
     2701 agccgaggcg ggccgtccgt acggcccttg gatcgatgcg ctccgccgac tgccagccga
     2761 cgccctcaca cacgccgatg gcaacgctct cgctccgctt ctcccggagc tcggcggatc
     2821 agccgccgaa gccggcgcga gccgcgatcg cttgttcggt gcggtcctcg acctcgtgac
     2881 ggccctggcc cgacgcgaag ctcccgccgt actcttgttc gacgatatcc actggtgtga
     2941 cgatgcatcg gcagcgcttc tgcactacgt cgcgcgaatg agcctcgagc atcctgtgct
     3001 ggtcgtgctc ggggcgcggg acggagagct catcgacaac gaatccgtca taaaaatgtt
     3061 gcggagcttt cgccgggagg gtctcatcga cgagcatctc ttgcttccat ttaccgaacg
     3121 agacgtcgag gcgctgattc ggtccatcga cggaagtgca gatgccaccg aggtcttcaa
     3181 tttgagcggt ggcaacgccc tcatggcgcg tgagcttgca cacgcgtcga gcgccgggga
     3241 aacggttcct ggatcactca aggagctgat cagcgatcga gttgaacggg tggctcccga
     3301 agctggcgac gtgcttcgat ggagtgctgt tctgggaact gcattcgacg tttcccgtct
     3361 ctccgagctg gtttcgctgg acttcgatcg actcatgagt gcgctgagag agctagagcg
     3421 ccatgccctc ctcgaaagct ctggcagctc cgcaaacatg tacgcatttc accatgctct
     3481 ggtccatcgt gtcgtgtact ccgagatctc ggagccgcgc cgaaagctca tgcacctgcg
     3541 agtcgcacga cttctcgcag aagcaaagga ccccgaggga gtcatctcgc tcgaattggc
     3601 tcatcatgcc tcggtcggag gtgatgccgc catggcggtt cgcgcctgcg tgaccgcggg
     3661 ggcgcggtgt cttcgagtat ttgcaaatgc cgaagcgtac acgcacgcgc ggcggggact
     3721 ccgcctcgtg gagcaactcg aggatccgga gcgcgcgaaa ctacgcatcg agctcgagca
     3781 gatcagtctc ggcgcacact ggcccgaaga cccagacgcc gagtccactc gcatcgaggc
     3841 cctggcagag caggcactcg acgcgggctg ccccgaacac gcccgtctgg ccttcacact
     3901 ggtgagcaca cttcgctggc acgaaggctc gctgagcgac gcccatcgct tgtcgctacg
     3961 agcggagctg gcaagtcggg gcaccaccga tcagcatcgc gcgatcggca tcgcagaaac
     4021 ggcccgttgc ctggtcattc tggaacggga tctcgcgcag gcggaggcac tcctcttgga
     4081 agcacgcgcg ctgatggacc gttcgggcga gcccacctgg gcggttttcg acggtgttgg
     4141 cctgcttcga ttgcatgccg gcgctttgga cgatgcgaag acagcattcg aagaggcctg
     4201 gatgctagcg aagaagagcg ggaaccgcct gcaagagttc atggcgctcg agcacctcgt
     4261 catgacctcg atccatcgcc gagatcctga cgaagcgtgt cagtatgcca aagagctcgc
     4321 agtcatcggc gaaaaacttc gtgagggcag cgagcgacca ctcgcgagag cgatgctggc
     4381 actgtgccgt tacaacagag gcgaggtcga ggatcgctcc gcgtttgaag aagctttgca
     4441 ggagctccgc gacgtggatg cgaagcagcg cctcgctttc gtgctcacgc aggccgccgc
     4501 ggtcgaagcg gagcggggca atctgcaaag tgcagctaca cgggctgccg aggccgagaa
     4561 gctcggtcaa atcctacaac agcccagtga gtccggtctt gcaagtgcgg tattgctgcg
     4621 gatcgcacga gagcgcggcg acagggcggc gatgcacgaa cgaatgcaag agctccgcgc
     4681 gatcagcggc tatgccaaac acgtcggtga gatcgtcgaa cgcgagctgg ccacggcggc
     4741 agccctctca gggacggagt tgcgagatat gggggcagag tgatggcacg cgtgatggtc
     4801 gagcggaaat tcgacgacgc ccaatcgttc gacgacctcc aggcgcaaga ggtcagtttt
     4861 gcctggtgtg ccgaacagca ccaggtgcga ttcattcgca gctacttctc acgcgacaga
     4921 aaactcatga tttgcgagta cgacgccccc gacgccgagt cagttcgcac gctccagcgc
     4981 acggcacgga tgccattcga gcgtatctgg accgcaatga tgttcgactg ggaagccgat
     5041 tccgaccttg agtgatcgta aggaacgcct ccgtcgcgag ctgcgacaac gcttgctggc
     5101 cgatcgccag gacttgccgg tcagcgcact ggcacgcttc agtcgcacgg ggtccacggg
     5161 cttgcgcatc ggacgcgcga tgctcgccgc gggacgaagc ggcagtgatg gggaggtcga
     5221 catcgagacg cttgtgaaga tcgtcggctc gatcgctcag ctcaagggta tcaccatgaa
     5281 gatggggcag atcatgagct acatcgacgt cgcccttccg gacgacctgc gcgacgccct
     5341 ttcggtgctt cagacacacg cgcagcccat gtctctcgag caagttcagg acatcttgcg
     5401 ccaagagctc ggcgcgcaag ccaatggcct cctcgataat atggaggcag cacctgtcgc
     5461 cgccgcctcg atcggccagg tccaccgatc ccgactacca agcggtgaac gggtcgccgt
     5521 caaggtgcaa taccctcaag tcgaaaaggc aatcgagagt gagctcaaac ccgcggccat
     5581 aggcaccgcg atctcggcaa tcgtctatcc gggcgccaag atcgacgact tcatcgccga
     5641 ggcgcgggcg cgcttcctcg aggagtgtga ctatattcat gaggctgctg cgcaagaccg
     5701 atttgcccag atctaccggg gccaccccca catcgcggtt ccgagcgttc acgaggcata
     5761 ctcctcccgg agagttctca cgtcgacctg gctcgaaggc atcggcttcg acgcctttct
     5821 ggcgggggcc ccaacgcagt cgatgcgcga ccgaatcgga gaggccctat tcggcttcta
     5881 cgtgggagcg ctcttcgaac atgcgctgta caactgcgac ccgcatccgg gcaactacct
     5941 catcctcgac gacggacgag tcggtatgct cgactacggc tgcacccgtg agttcgaccg
     6001 gagcttcgtc gtgcaactcg cgcgactcac gcgagctgtg cacagcgacg aacacgctct
     6061 gctgcaccga gcctttttag acctcggtat ggtccgcgac gggcaatcgt acgatttcga
     6121 taccgcgcgg gatctggtgc gctcgttcta cgggccgatg cttcgcgatg cggtgcaatc
     6181 gatcgacctc ggcgaagcca tcggcatgcg acgggtcttc gctcgcaagc gtcagctgct
     6241 gaagctccgg ctccccggag agtttctctt tctcttcaga atccggttcg gcctgatgtc
     6301 ggtgctgcag cgattgggag ctcaggccaa ctggtacaat ctcgaacggc aatgcatcgc
     6361 agccatcgct tgaggaatgc gaacgagccc gccgagctcg tagccggtgg tcgatcgtcg
     6421 ctggtccaag ccgggcttcc atgcgaaact ccgatttcat ttctaccttt ggtcttgcaa
     6481 tgactcagtc gggattgaca tgtcggctgc tcggtccgct tcggatcgag cgcaatggca
     6541 aagcaatcga gcttccccgc tcgaagaaga cgcgtgccct cctcggctac ttggccgcca
     6601 ggggtggcga acaccgccgc cagcggctca ccgacctctt ctgggacgtg gccgacgatc
     6661 cacgcggtgg gctgcgttgg tccctaagca ggcttcgcgg attggtcgac gaaccgggtc
     6721 gagctcgcgt cgtcgcagat cgtgattgcg ttcgactcga tctcagtgac gccgatgtgg
     6781 atctgcatcg tgttcaagcg gccaagaccg gcttggggac actggacacc gacaccgtgg
     6841 gtgagctggc tgcgctgttt caaggcgagc ttctcgaagg aatcgacctg cccgagttta
     6901 cggagtttca agcctggttg accgccgtgc gcggggattg tcgccagctc cgcatccagg
     6961 tactcgacga gttcgtcggt cgatcaagtg acgatctcga gagagcgctt ccatgcgcac
     7021 gcgatttggt tcgtcttgat tcgttgaacg tccagcgatg gcttcatctc ttggacctgc
     7081 tcgagcgcac ggggagaggt cgcgaagcgg agcaacagat cgaggtgggg cggcgcacgc
     7141 tgactgaaca aggcatcgac gacggcgcac tggtggaggc aaagcgcgtg atgctgggca
     7201 acccgagagc tcggttaaaa tcgcctcgcg cccaggagct tcgccaggag atccgtttct
     7261 gcacctcgtt cgatggcact cgaatcgcgt atgcgaccgt tggcgaggga aggccgatcc
     7321 tcaaagcagc caattggctg acccacctcg aattcgactg ggagagccca gtgtggcgtc
     7381 acgtgttcac cgagctgagc cggaacaact tgctcgtccg atacgacgat cgcgcaaatg
     7441 gactttcgga ttgggatgtc gaggatatct cgttcgacgc gttctacaag gatctcgaag
     7501 gtgtcgccga cgcgctcaag ctcgaccgtt tcgcgctctt gggcatcagc aagggtgcat
     7561 ccgtgtccgc ggcctacgcg gcaaggcatc ctgaccgcgt atcccacctc atcctccttg
     7621 gaggatttgc aacgggaagg ctcgtcgaag ccagcgagcg atcgcgtgag cacgaaatgg
     7681 caatgcgaac gctcatgcgc gtgagctggg gcgccgacaa cccggcgttt cggcacctct
     7741 tcaccagcac gttcatccca aacgccacgc ctgagcatgt gaaatggttc aacgatctgc
     7801 aacgaatcag cgcatcgccg gacaacgcgc ttcggcttcg catcgccgcc gcggatatcg
     7861 acgtacggag cttgcttccc aagatccagg cgccgacctt ggtcatgcac tcgagaggcg
     7921 actcggcggt cgagtatggg cgagggctcg cactcgccgg aggcatcccg aacgcgcgtt
     7981 tcgtcacgct cgaaagcgac aaccatctgc tcactgaaga cgagcccgcc tggacgaagt
     8041 ttctggacga ggtgcggcga ttcctaggtg aatcggagaa cggcgcctga ctgtaccaag
     8101 agcctcggct cggagcctcc atgcaaaacc aagtcctaca tgtcctcacg gtgttcatgg
     8161 gttttttcgc catcatgaac cctattgcca acacgccgat cttcctcgga ctcaccgagg
     8221 gggattcggc cgacatccgg agagcggtgg ctcgacgcgc gttgctcgtg acgttcctga
     8281 tcatcgtggt cttcacgatc tcgggtcgca tgatcttcga gcttttcggc atcggacttc
     8341 cggccttcca gatcaccggt ggcctgcttg tgcttctgat cgggttccat atggtgcagg
     8401 gtcatgcgtc cagggttcag caccctggca atgccggagc cacgcccggc ctcgaggggg
     8461 agctcgacaa ggccatctcc ccgcttgcga tgccgctgct cgcgggtccg ggcaccatct
     8521 cgacggcgat caatttcgct tctcagggcg gcatcggccc agtggtgact actctggtca
     8581 cgttcttcgc cctttgcgtg atcacctatt tctttttcgt ttacggagaa cggctggtgc
     8641 ggtacctcgg acacgccggc atgagcatca ttacgcgcct gatgggactg atcctagccg
     8701 tgatcggcac ccagatggtc atcggcggca tcacgggcgc gatcaagctc gctgcgtcat
     8761 agtgttcgaa ccgagctgga gcctcatgaa cccacgcatc gtttcactcg gcgccatttg
     8821 gacgacgtgc ctgccactga gcgtggttct gctgtcgtgc gtgtctcaga cccccgctgc
     8881 aaagcctgct caagcgacaa gccccgaatt cggcatggcg caggacatcg tttcgtttgc
     8941 ggctgcggtg agcaaatctt tgaacgaccg ctccgccaag ccagctccca gcaatactta
     9001 cagctacgag accgcatacg gtccggacga gcttgccgag ccgccggtgt acctggctcc
     9061 caccgcaacc tactccggac tcgcttcgac ggactgctcg ggctgggtga gcttcgtcgt
     9121 caacacggtc tcccctttgc acgaagccgt gctccaatcg cagggccgat tgcctgctta
     9181 caaccaggtc tacccagacg gcttcgcctt ggaagaaggg cagcgaccct ggtcacgagc
     9241 attcgtgctc accaatttct ttcgcgcgga atacgcaaaa tcctcaggac ttgcgcccgt
     9301 accgcgtttc gaagagctgc gatcgggaga catcggcgcc tacgcgatgg gacgatacgt
     9361 cgacccttca gacgcaagcc tgatcaagac cacggacacg ggacacacct tcatcgtgat
     9421 gggcccaccg tcgatcgtgg atccgaacac ggctgactat gacggacagg gcactctctc
     9481 cgcccaggca gtcaaagtga tcgcggttcc ggtcatcgat tccagcgccg cggtgcattt
     9541 cgatcctgac gcgcgaaaga atgagcaagg ccagtattcg ttgccgaaga gctcgcccta
     9601 cgcagatgcg aagcctggtg gggttgggat cggtacgctc tggtttgccc tgggcgaaga
     9661 cggtcgcgtg ctgcagcgcc ggctcgggcc cggagaaacg tacgccgatg tcgtggtggg
     9721 cgccgcgcgc ttgcagaacg tcatctcatt gcagccggag atcttggatg agcgaggcga
     9781 tttgctcgtc gagatcttcg acaactcccc gtccgagttt gatggctctt cgtttggccc
     9841 cgcgccgatc gatctgaccg gaccgggggg catccgcctc gccgacggac gcctcggctt
     9901 gaatgggcga agcgacttct ctggaggggt gacggtggat tcgggagagc tcgtggtcga
     9961 gtcggatggc gcactcggga ccggcgacat cgatgtccgc gggggagcgc tgacgctccg
    10021 gagtccggcg ctcggagaca cggcagccct tcagctctcg gacgacctgg aagacggcgc
    10081 ggttcgtctc gacttttcgg ggactgacat cgttggcacg tttcggatcg gagacaaagt
    10141 gcaccgctgc ggaacctggg gagctgttgg ctcgggcgcc atgtttaccg ataccgtgtt
    10201 ctccggtgca ggcatccttc aactcgcggc cgagccgatc gaaagttgca gtctcaaaaa
    10261 ctgaaggacc aaggcccgcc atctcgtggt aggttccgga accgtgacgg acatctcgac
    10321 ctcggcttcg gttctcatcc cccgcccgcg cgacgaggtc tttgcacggg ccaccgacag
    10381 cacgaacacg cccgagacct tgcgcgcgcg tggaccgttc gcagggatca ccagggtcga
    10441 aatggatgaa gggcaagtcc tcgcaaaggg aacgaagcgg cgcgtcttca tgaccgacgg
    10501 cacggtgctc gaggaagtca tcctcgacta tgaccccccg gtccggcatc gctatcgctg
    10561 gacgggcggc gccaagttcc cgttctcgtt gctcgtgcgg agcggcaccg gcaactggga
    10621 cttcatcgaa acggaaggcg gcacgcgcat cgtctggact tatacgttcg gcctgacctc
    10681 cccgctcgcc tatccgctcg cgattcccat cgtcttgctc ttcaaacgct ggctgcaaca
    10741 gggcctagac gccatccgtg cagagctcgt cgccgagcca gagccgtgaa tgcgttttcc
    10801 aaatcgagac tccctgtgcg ttatcgatgc gatgacctca tgagcaagac acggctcgat
    10861 ctgccattcg tcttctctgc actgctggcc ctgtccgctt gcggagaggc ggtgaccgtc
    10921 gaggaccagg gaggcgccag cgggacagca ggtgcgggtg gcggcgggga caccggcggt
    10981 cggggtatcg ggggtagcgc tggcagcgaa aataccgggg gagccggtgg cgtgtcgtac
    11041 ctggtctgca cccttgggct ctgcatggaa gacgaggcgc tggccagatc ttgccaagag
    11101 gtatacgacg tatgcgtagg tcagggtcac tacacgaggt gttgcaggat ggatgccgac
    11161 aagacctgcg gcgtgtttgg agaaacgggg ccctactagg ccatccacac tgccggaagg
    11221 agagctcaat gaggatagcg gtttcgacat tggtcgtgtt ggctgcggcc atcgctcact
    11281 cgggatgcac tgtcgcggaa gatgaaagcg gcgaaggagg cagcggagcg ggaggtggag
    11341 gagaaggagg aggcggtggt agtggtggct ccgctggaac gggcggtgca aacggaaaca
    11401 ctggaaccgc ggcggtcgcg gggccggaga cctgtgacct cggagccgcg gatcgactcc
    11461 cgatcgcaga tgcctacatc actggcgacg gtcccgaagc ggaccgcatg ttggctgaca
    11521 cgaacttcgg gggcgcaacg actctgcttt gcaagtccgc ggcgaatctg ggcttcacac
    11581 ggaagaccta catggtgttc gacctgagtg atcagtcagc ggctgttcag tcagcgaccc
    11641 tcgtgctcac cttggagcgc cacatcgagg gcccgacacc ggagacgtcg gggccgcaac
    11701 cgttcaactt ctacggcatc atcgacgacg aggactggga cccagccacg cttcccgaaa
    11761 cctccacgac cgatggcatt acttggaaca acgcacctcg gaacaaaaac gactggagag
    11821 tcccgtttga ggacagcccc ggtgttcccc tgctgatcgc cgggtatgac ttcatgcttg
    11881 ggggagattt cgaccaagat gcggacggcg tagacgaccc agggacgcgc tacgccttcg
    11941 acctcaccga ctacatcaac gagcgcatcg ccaacgacgc agacggcaag gtgtcggttc
    12001 tcatcgcgca cgacaaccaa accggcgtga atgtcgatac ctccgtgttc ttctccaaag
    12061 agtacgctga tgacgagtgc gaccgcccgt tcctgcggct cgagtagctc aaaatgagtg
    12121 ctacaagtca gtggtgaccc gtggcacccc taagcgcccc ttcgagaacc gaagccggcg
    12181 ctacctcgaa gagctcctcg cctgccgcgg tgtcgaagaa gacctcgagc tgcgctcccg
    12241 cttcggcttc atggcgtttc acggaggaag tctcgagacg caaaccgacg cgattgccgc
    12301 aggggcggcg cagaagtcag gcgcctcttt ctatgcggta cggcaacccg cccatctccg
    12361 ctggcacatt ccatcgtcgc tctttcgcgc ggacgagtcc gacaagctcc aagcattctt
    12421 cgagagcgtc gacgtcgtgg tgaccgtgca tggctttcga cgcaaggcct tcccccacca
    12481 cgtcatgctc ggcggacgta atcggaccct cgccgcccat ctcgccgaaa ccctcaggcc
    12541 cgagctccca ggcgcggtag tcgtcgacga cctttcgaag atcccgagct cgattcgagg
    12601 cgtgcatccc gacaacccgg tcaacgtccc ccggctccgc ggcgtgcaaa tggagctttc
    12661 cccgctgctt cgcacccagc cggagaacct agtcgaagcc ctcgcacaag ccgcccgcac
    12721 gttttcgcct gcttagcgaa acagcaagcc aacggaatcg catggattgc gccgcaacgc
    12781 ggcaccggag cgtgttggcc cggacgccga agcactggcg ttcgcgttgt ggcacgtgat
    12841 caaggtcgtt tgacgcgaga agaacgtgtc caggcggcat cgcgggcgaa gggatcgaaa
    12901 acggaggacg cgtatcgata acgagcagca tcggagacac gcgtttgaag tttccgatcg
    12961 agctctgaag atcggtgcca acgcgaggcg ccgggcgaca ctctaggatt gcgcaaccca
    13021 agggttgcgg tagcctcgac cgaaggaacg tgcagaacct cagcgataca aggctctttc
    13081 gccagcggtg ctatgtggat ggcgactgga cgcaggcgga gggcggccgc agcgtggcgg
    13141 tgaccaaccc agtgaacgga gaggtcatcg gacacgttcc cgattgcggc gcggcggaga
    13201 cgcgcaaggc gttgcaggcc gcggagtccg cccttcctgg ctggcgcgcg ctgccggcga
    13261 gggaacgcgg cgtgaagctg cgcgcctggt tcgacctgat gatggcgaac caagatgacc
    13321 tcgcgcggat catgacgatc gagcagggca agccactcgc cgagtcgcgc ggcgagatcg
    13381 cctacgcggc ctcgttcatc gaatggttcg ccgaagaggg ccggcgtaca tacggcgatg
    13441 taatcccaca gcaccaggcc gacaagcgga tcatcgtgat caaacaaccg atcggcgtct
    13501 gtgtcgcgat cacgccttgg aacttcccag ccgcgatgat tacgcgtaag gctggagctg
    13561 ctcttgcggc cggttgcacg atggtggtca agccggccct cgagactccg tattcggcac
    13621 tggcgctctg cgagctcgcg gagcgtgccg gaatcccagc aggcgtgctg aacgtcgtca
    13681 ctggcgacgc gaagtcgatc ggtggcgaaa tgacctcgag cccgatcgtc cgaaagctca
    13741 gcttcaccgg ctcgacagcg gttggaaagc ttctgatgga gcagtgcgcc ggcacggtca
    13801 agaaggtctc actcgagctc ggaggcaacg cgccttttct cgtcttcgag gacgccgatc
    13861 tcgatgccgc cgtcgaaggc gccatcgcgt cgaaattccg gaacgccggt cagacctgcg
    13921 tctgttcgaa ccgttttctg gtgcatgacc agatctacga cgagtttgcg cggaggcttg
    13981 ccaacgccgt gagagagcag ctcaaagtcg gcaacgggct cgacgagggt gtggcccagg
    14041 gcccgctgat cagcaaggca gccgtcgaca aggtcgagag gcacatctcc gatgccgtca
    14101 gtaagggcgc caaagtcgcg atcggcggcg cccggcatgc gctcggcggc accttcttcg
    14161 agccgaccgt gctcaccgac gtcaccccgg ccatggccgt ggcccaggaa gaaacttttg
    14221 gtccgctggc gccgctcttt cgcttcgagt ccgaagagga ggccgtgcgc atggccaatg
    14281 acaccgaata cggcctcgca tcttatctct acagccgcga cgtctcccgt gtgtggcgtg
    14341 tcgccgaagc gctcgagtac ggaatggtcg gcatcaacac gggcctcatc tcgacctcca
    14401 ttgcgccatt tggtggggtg aagtcatccg gcatcggccg cgaggggtcg aagtacggca
    14461 tcgaggacta cctcgagatc aagtacatgt gcatcggggg catcaacccg tgaagcgaat
    14521 gacatctcgg tagacgaagg agcccgatgg cgatggcgac gaactcggaa cttctctcgc
    14581 gacgaaacca ggccgtggca cgcggtgtcg ccagcatgca tccccgcttc gccgagcgcg
    14641 ccgagaacgc gctggtctgg gatgtcgaag gcaaacgcta catcgacttc gccggcggca
    14701 tcggcgtcct caacaccggg caccggcatc cgaa
//
LOCUS       DASRZF010000040         4451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2435203, whole genome shotgun sequence.
ACCESSION   DASRZF010000040 DASRZF010000000
VERSION     DASRZF010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2435203"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..529)
                     /locus_tag="VFG22_01815"
     CDS             complement(50..529)
                     /locus_tag="VFG22_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415003.1"
                     /translation="MNWDAIGAIAELLGGLATVATLVYLARQIRESGRSTRLAAMQSA
                     MLAEQTAMEIPARDRNLARVIPIAVGIADGTLTEEEHQQFRFWLFLMVRSHENMFVQH
                     QAGMIDDETWAARSSTALFLLASPIGRRIWEGASNTYRADYQAWVASSQIRKDAPAV"
     gene            779..1714
                     /locus_tag="VFG22_01820"
     CDS             779..1714
                     /locus_tag="VFG22_01820"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HET6415004.1"
                     /translation="MQKTALVEVDVSAKTLAVIIEQNEQRGSVLEFANDGIGHRKLIT
                     VITKRGRSARLVLEATGNYSLDLAFALHRAKRVEVMVANPRALAQFAGALLRRSKTDA
                     IDAEVILELAKWMLFAAWSPPDPECMDLQAISRRIEAMTRNATQEKNRLRAADAFAET
                     SDIARNDIEVNIRHLEKRIARLRDQALALIEDHGELSRHFEHIMSIKGIADAAGIQIL
                     AEIAVLPKDMSVREWVAHAGPDFRQFQSGTSVNKPARISRRGNRHIRRGLFMTSIVAV
                     QYEPHLRAFYEKLLARGKTKMQANVAVMRKLLHAI"
     gene            complement(1950..2324)
                     /locus_tag="VFG22_01825"
     CDS             complement(1950..2324)
                     /locus_tag="VFG22_01825"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6415005.1"
                     /translation="MANHVPAGWPAVVPRIAVADPEALVGFIQRVFGAVGKFHRERPS
                     ELRIGDSLIMVGSTIGREAMQAFLYVYVEDTDAVFRRAVDLGAKSLEEPQDMPYGDRR
                     AMIRDPWGNTWQIATHGGRFTP"
     gene            complement(2487..3269)
                     /locus_tag="VFG22_01830"
     CDS             complement(2487..3269)
                     /locus_tag="VFG22_01830"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6415006.1"
                     /translation="MPEPTGWRLDDAAAEYFEGHFVPAIFAKWAPILVDAGGVAQGQR
                     VLDVACGTGIVARVAADRLAGVGEVTGLDLNPSMIKVARRLQPDVNWHEGDATDMPLP
                     DASFDAVLCQAGLMFFPDPVTALREMRRVLRSGGQLAVQVWGRSEGYDSTAELLEETS
                     GKEIADIFRAPFSMAQPDLLPELLAKAGFDSPQSETYEKPARFPSIEDFLRTEIDGWV
                     LKGLVDVDALTPGARERLKPYADTDGTVAIPMAGHVVTCAKR"
     gene            complement(3470..>4451)
                     /locus_tag="VFG22_01835"
     CDS             complement(3470..>4451)
                     /locus_tag="VFG22_01835"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HET6415007.1"
                     /translation="PAAQRRNLDVMTDALVTRIVIEGGRATGVTIRRAGTEQTQSART
                     IVLSAGTYASPQILMLSGIGPAAQLRNFEIPVLVDAPEVGENLQDHPILPIVYGTTNT
                     DTLLRAESPRELLRYLLFKRGMLASNGVEGFAFTQVEPGPVDAPDLELMFLPFEMREQ
                     FLEPPTRHAFGLGPAVVAPRSRGRVILRSNDPLVPLGIDPALLSDGEGIDARVLFEGV
                     RLSRTIAATSPLAELNTGELRPGADVQSDRHLLDYASTELQTVYHPTSTCRMGSDARS
                     VVDSRLRVRGIEALWIADASVMPAVPRGHPNAVVAMIANRGAAWIEEAVS"
BASE COUNT          869 a         1401 c         1394 g          787 t
ORIGIN      
        1 accacggatc gattggaacg agctgttggg cgtcagctgc aaccgctcgt tagacggcgg
       61 gcgcgtcctt tcgtatctgg gaagaggcta cccaggcctg gtaatcggcg cggtacgtat
      121 tcgaggcgcc ttcccaaatc ctgcgaccga tcggcgacgc tagcaagaac agggcagtgc
      181 tggagcgcgc agcccacgtt tcgtcatcga tcatgccggc ctgatgctgg acgaacatgt
      241 tctcgtggga gcgcaccatg aggaacaacc agaaacgaaa ctgctggtgc tcttcctccg
      301 tgagggttcc gtcggcgatg ccaacggcaa tcggaataac gcgcgccaaa ttgcgatctc
      361 gggccgggat ctccatcgcg gtctgctcgg cgagcatcgc agactgcata gccgctagtc
      421 gtgtcgatcg gccactctcg cgaatctgtc gcgcgaggta tacgagtgtt gcgaccgtcg
      481 cgagcccccc gagcagctct gcgatggcgc cgatggcgtc ccagttcact caggctcccc
      541 gccgtcttgt atatcgatcc aatgctctcg agtacgacct tcgtacactc caagtgatca
      601 gccgggtgag gccgacggcg catgaagcca agagcttgac cgtcagattg gccccccgcc
      661 ctcttcagcc atgccgtgga gtatccgagg cccgagagcc tgcgtttaca agacgagtag
      721 gtgatggtgc agggtcgggg aaccggcagc cattgtagtc gagcatcagg agaatgtgtt
      781 gcagaagacc gccctagttg aagttgacgt cagcgcgaaa acattggcgg tgatcatcga
      841 acagaatgaa cagcgagggt ccgtgctcga gttcgccaac gatggtattg gtcaccgcaa
      901 gctcatcacc gtgatcacga aacgcgggcg ctcggcgcgc cttgttctcg aagctacagg
      961 aaactacagc ctcgacctgg cgttcgcgtt gcatcgagcc aaacgggtcg aggtcatggt
     1021 ggccaaccca cgcgccctcg ctcagttcgc cggagccctt ctccggcgat cgaagaccga
     1081 cgcgatcgat gctgaagtga ttctcgagct cgcgaaatgg atgcttttcg cggcctggtc
     1141 gcctccagat cccgagtgca tggacctaca ggccatttca cgtcgaatcg aggcgatgac
     1201 gaggaacgcc acgcaagaga agaatcgact tcgtgctgcc gatgccttcg ctgagacaag
     1261 cgacatcgcg cgaaatgaca tcgaggtcaa catccggcat ctcgaaaaga ggattgcacg
     1321 attgcgcgac caggcgcttg cactgatcga ggatcatggc gagctctcgc gtcacttcga
     1381 gcacatcatg tccatcaagg gcatcgccga tgccgcaggg atccagatcc ttgcggagat
     1441 cgcggtgctg cccaaagaca tgtcggtgag ggagtgggtc gcccatgcgg gacccgattt
     1501 ccgacagttc caatcgggca cgtcggtcaa caagccggcg agaatcagtc gacgtgggaa
     1561 tcggcacatc cgccgggggc tcttcatgac gtcgatcgtt gccgtgcaat acgaaccgca
     1621 cctaagagct ttctacgaga agctgctcgc cagaggcaag accaagatgc aagcgaacgt
     1681 cgcggtcatg cggaaactcc tacacgcgat ctaaggaatg atgaagcacg accgagtctt
     1741 cgaaggggag aagttcttcg cgctccgcga ttgacagcga atagagtatc tgacggattg
     1801 ggcgctcagc tgcggacaac agcgccgtcg aaggtcgctg agccacccag gttgaatgcc
     1861 agaacgctac caaaccccaa ttggaacgct ctgttggccg tcagctgcaa cgcctctctc
     1921 atggcgcgcg gcctcttatg gcgcgcggcc tacggcgtga accggccacc atgggtggcg
     1981 atttgccacg tgttgcccca aggatcgcgg atcattgcgc gccgatctcc gtacggcatg
     2041 tcttgcggtt cctcgagcga cttggcgccg agatcgactg cacgccgaaa gacagcgtca
     2101 gtgtcctcga cgtagacgta gagaaaggcc tgcatcgcct cgcgcccgat ggtggagccc
     2161 accatgatga gagagtcgcc gatccgaagt tccgacggcc gctcccgatg gaacttgccc
     2221 actgcaccga agacgcgttg gatgaaccct acgagagcct cgggatcggc gacggcgatt
     2281 cgggggacca cggcgggcca gccagcgggt acatggttcg ccatcctcga ctccacgccg
     2341 cccaacggat cggcgttcag cggcttgcgg cgacctggct cgcagccccg gaacacctcg
     2401 gatgtcgcga ttctaccacg caccactagg catcaagcgt tggcaagtcc gctgcaacgg
     2461 cacttgttgg gcggcaggcc tcgcggctag cgctttgcac aggtaacaac gtgccctgcc
     2521 atcgggatgg caaccgtgcc gtcagtatca gcgtagggct tgagtcgttc tcgagccccg
     2581 ggtgtcagtg catcgacgtc tacaaggccc ttgagcaccc aaccgtctat ctccgttctc
     2641 aggaagtcct cgatcgaggg gaatcgcgct ggcttctcgt acgtttctga ttgcggcgag
     2701 tcgaatccgg ccttcgcgag gagttcgggg agaaggtccg gctgcgccat cgagaagggg
     2761 gcccggaaga tgtctgcgat ctccttgccg gaggtctctt cgaggagctc ggcggtcgaa
     2821 tcgtaccctt cgcttctacc ccacacctgt acggccaact ggccacccga gcggaggacc
     2881 cgccgcatct ctcggagggc ggtgactgga tcggggaaga acatcagacc tgcctgacaa
     2941 agcactgcat cgaagctcgc gtcgggaagg ggcatgtccg tcgcatcacc ctcgtgccaa
     3001 ttgacgtccg gctgcaggcg ccgcgctacc ttgatcatgc tcgggttgag atcgagaccg
     3061 gtgacctctc ccacccccgc gaggcgatcg gcagcgacgc gagcaacgat gcctgtgccg
     3121 caggccacat cgagaactcg ctgaccctgg gccactccac ctgcatcgac caagataggc
     3181 gcccacttgg caaaaatggc gggcacgaag tggccttcga agtactcggc ggcggcatcg
     3241 tccaaccgcc accccgtcgg ttccggcatg agtcccgccc tccgaatttc gcgtgagcag
     3301 accggtcgtc ctgcagccca acggatcggc ggttcagctg cgagccacag cgcctccgtg
     3361 gcttgaccga agcgcccatg tttcaatgcc agaccttacc agaggtcgat tgggccgaag
     3421 ccgcttcagc tgcagcgcct gttgggcgaa ccgcgtcgcc aggcgacgct caactgaccg
     3481 cctcctcgat ccacgcagcg ccacggttgg caatcatcgc caccacggcg ttcgggtggc
     3541 cacgcgggac cgccggcatg acggaggcat cggcgatcca gagcgcctcg atgccccgga
     3601 cgcgcagccg cgagtcgacc acagagcgcg cgtcagaacc catgcggcat gtgctggtcg
     3661 ggtgatagac ggtctgcagc tcggtgcttg cataatcgag caggtggcga tcgctctgca
     3721 catccgcacc cgggcggagt tcgcccgtat tgagttcggc caacggcgaa gtcgcggcga
     3781 tggtccggct caagcggacg ccctcgaaga gcactctcgc gtcgatgccc tcgccgtcgc
     3841 tcagcaacgc cgggtcgatc ccgagtggga cgagcggatc gttgcttcgc aggatcacgc
     3901 gtcctcgaga tcgcggcgcg acgacagccg gcccgaggcc gaacgcgtgc cgagtcggcg
     3961 gctcgaggaa ctgctcgcgc atctcgaacg gcaggaacat gagctcgagg tcgggcgcat
     4021 ccacgggacc gggctcgacc tgggtgaacg cgaacccttc caccccgttc gacgcgagca
     4081 ttccgcgctt gaacaacaga taccggagca gttcgcgggg tgactcggct cgcaagagag
     4141 tatcggtgtt ggtggtgccg tagacgatcg gaaggatcgg gtgatcctga agattctcgc
     4201 ccacctccgg cgcgtccacg aggaccggta tctcgaagtt tcgcagctgg gccgcggggc
     4261 cgataccaga caacatcagg atctggggcg atgcgtacgt ccctgcggag agcacgattg
     4321 tccttgcgga ttgggtttgc tcggtcccgg cgcggcggat cgtgacgccc gtggcgcgcc
     4381 ctccttcgat cacgatccgg gtgaccaacg cgtcggtcat cacatcgagg ttccggcgct
     4441 gcgccgcagg c
//
LOCUS       DASRZF010000041         2983 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_716952, whole genome shotgun sequence.
ACCESSION   DASRZF010000041 DASRZF010000000
VERSION     DASRZF010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716952"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="VFG22_01840"
     CDS             <1..887
                     /locus_tag="VFG22_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415008.1"
                     /translation="PWMAAVTAALIIRVWRMGWGSKLALSALVATQILVGSDVYFMQT
                     HAMIRSPIKKVNDLLSAGYEKRYEQRLDVFKSWTRARDAIPDDAHVLLHDNHVHLGLS
                     RATTSDWGAWQFGISYGRIRSPAELWELYRELGVTHLLWEDRTSKGWDSIAGDLVFFD
                     FALNRTTGRKSVGNLRIGSMPTEPPLDAAYGRVAYLGCGTTYATGLYELSQMTTPVFG
                     PEKESFAEPIQPAPLEDVAGLLDEASFVVLDPDCAGKLSGDVARAGFALGASRLQINP
                     RRRTKLQLYVKASPSRRP"
     gene            complement(892..1833)
                     /locus_tag="VFG22_01845"
     CDS             complement(892..1833)
                     /locus_tag="VFG22_01845"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HET6415009.1"
                     /translation="MAKSRIRWVMRWVGPLLLIWLVWRFADLHTLADTLARARAWPLV
                     IAVALDAVVIHAKLWRWRSLLRSAGVSLSVSRAYMAFLPSLYLGLATPGRVGDALRIQ
                     YLKRDHAVGYADGLAVSIIDRLCDVYVLLAFVALGIVHLARTASIPFAQTTWLAVAAV
                     AIAPAFLFVRRVAEPAAQWLFGRFSKGQIHEGLSVFFDALRSQIGWNLVGPALLTVLA
                     FLTNYLQAWLVANALGIDLSFIDVSALVAISSLLSLLPISISGVGVRESFFALVFPAF
                     GLAATLGIAFGLGFFAVVYVPTLVLGFIAWLAWPPSN"
     gene            complement(1846..>2983)
                     /locus_tag="VFG22_01850"
     CDS             complement(1846..>2983)
                     /locus_tag="VFG22_01850"
                     /inference="COORDINATES: protein motif:HMM:NF021379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HET6415010.1"
                     /translation="LGRDRLGTCASGLILALAYALHPALHGANMYEFHSLTLLFSPLL
                     FALYFFLSGKLRLYYAMLALLLLIREDVALLMCFVGFYGVISGSTEYKRAGWATIVAC
                     LTYFVLVKTVFMTSLDIFNQGPESYGFSYYYAEMIPNKWGFGDFLITLLTNPAYIVAL
                     VTKEAKLHYLLVIFLPTLMLAAWAGKKRFMLLYGLIFTLLASKPPVFSWHFQYSAVLL
                     PVAIALAPEGLLRLQKARLRDHSFTAAVMGCVLVASLLVSWKHGGIVENAAFRGGFSK
                     VTRSLSPEKEAAYQSFTELISKIEPDASVSSSARVGPHVSNRAEVYQVEQDIETDYVL
                     IDTRDLRSGVKERIKRLEEAKKIELVERSRTWALYRATPPPPVE"
BASE COUNT          657 a          893 c          941 g          492 t
ORIGIN      
        1 tgccttggat ggctgcggtc accgctgcgc tcatcattcg ggtttggcgc atgggttggg
       61 ggtccaagtt ggcgctcagc gcgctcgtcg ccacacaaat cctcgtcgga agtgacgtgt
      121 atttcatgca gacgcacgcg atgattcgat cccccatcaa gaaggttaac gacctccttt
      181 ccgccggcta tgagaagcga tacgagcagc gtctcgacgt cttcaaatcg tggacgaggg
      241 cgcgtgacgc cattcccgac gacgcgcacg tattgctgca cgacaaccac gtccacctcg
      301 gcctatctcg cgctaccacc tccgactggg gggcctggca attcgggatc agttacgggc
      361 ggattcgatc gcccgcggag ctctgggagc tctatcgcga gttgggcgtg acacaccttc
      421 tttgggagga ccgaacctcg aagggctggg actccatcgc aggggacttg gtgttcttcg
      481 actttgcgct caatcgaacg actggaagga agagcgtcgg aaaccttcgg atcggaagca
      541 tgccgaccga gccgcccctc gacgccgcgt acggcagagt cgcatacctg ggctgcggca
      601 ctacgtacgc gaccggcctg tatgagctct cccaaatgac tacccccgtt tttggaccgg
      661 agaaggaaag cttcgccgag cccattcaac cggccccgct cgaggacgtc gctgggcttc
      721 tcgacgaggc gagcttcgtc gtgctcgacc ccgattgcgc tggcaagctc agcggcgacg
      781 ttgctcgcgc agggttcgcg ctgggcgcat ctcggctaca gatcaatccg cggcgaagga
      841 cgaagctgca gctctacgtc aaggcctcgc cgtcacggcg tccctgaaca cctagttcga
      901 gggcggccag gcgagccagg cgatgaagcc gagaaccagc gtcggaacgt agaccactgc
      961 gaagaagccg aggccgaacg cgatgccaag agtcgcggcg agcccgaacg cgggaaaaac
     1021 cagcgcgaag aaggactcgc ggaccccaac accggagatc gaaatcggga gaaggctcag
     1081 caaggagctg atcgccacga gcgccgagac gtcgatgaag ctgagatcga tcccgagcgc
     1141 attcgccacc agccaggcct gaaggtagtt ggtgaggaaa gccagcacgg tgagcagagc
     1201 ggggccgacg agattccaac cgatctggct gcggagcgca tcgaagaaga cactcagacc
     1261 ttcgtgtatc tggcctttgg agaaccgccc aaagagccac tgggctgccg gctcggcgac
     1321 ccgtcgcacg aaaaggaacg caggggcgat agcaaccgcg gcgaccgcga gccaggtcgt
     1381 ttgcgcaaat ggaatcgacg cagtgcgcgc tagatgcacg atgcccaggg ctacgaacgc
     1441 aagcaggacg tagacgtcac aaagcctgtc gatgatcgaa accgccaagc catcggcgta
     1501 tcccaccgcg tggtccctct tgaggtactg aatccgcaac gcgtctccga ccctccccgg
     1561 agtcgccaaa cccaggtaga gggagggaag aaacgccatg taggcgcgtg aaacggacag
     1621 cgacacgccg gcggacctca gcaagctgcg ccaacgccag agcttggcgt ggatcacgac
     1681 cgcatcgagt gccacggcga tgaccaacgg ccaggcccgg gcgcgtgcca gggtgtccgc
     1741 aagtgtgtgg aggtcggcga agcgccaaac caaccagatc aggaggagcg gcccaaccca
     1801 gcgcatgacc caacgaatgc gtgatttcgc catggcttct tggcgctatt cgaccggcgg
     1861 tggcggcgtc gcgcgataga gcgcccaggt acgactccgt tcgaccagct cgatcttctt
     1921 ggcctcttcc aagcgtttga ttctctcctt cactcctgag cgaagatccc gggtatcgat
     1981 caggacgtag tcagtctcga tgtcttgctc gacttggtag acctccgcac ggttcgaaac
     2041 atggggccct acgcgggcgc tagacgagac cgatgcatcc ggctcgatct tgctgatgag
     2101 ctcggtgaac gattggtacg cggcctcctt ttccgggctc agcgagcgcg tgaccttcga
     2161 gaaaccaccg cggaaggctg cgttttcgac gattccaccg tgcttccaac tcacgagaag
     2221 actcgccacg aggacgcaac ccatcactgc cgccgtgaag ctgtggtctc tcagccgtgc
     2281 cttttggaga cgaagcagcc cttcgggggc cagcgcgatg gcgactggaa ggaggactgc
     2341 agagtattgg aagtgccagg aaaatactgg gggcttcgag gcaaggagcg tgaagatcag
     2401 gccgtacagc agcatgaagc gcttctttcc cgcccaggcc gcgagcatca gcgtagggag
     2461 gaatatgacg agcaagtagt gaagcttcgc ttctttcgtg accaacgcca cgatgtaggc
     2521 tgggttcgtg agcaaggtga tcaggaagtc accaaagccc catttgttcg ggatcatctc
     2581 cgcgtagtag taggagaacc catacgactc ggggccctgg ttgaagatgt ccagcgaagt
     2641 catgaacacc gtcttcacga gcacgaaata cgtcagacaa gcgacgatcg tggcccatcc
     2701 tgcacgcttg tactcggtgc tacccgagat gacaccgtag aaaccgacga agcacatcag
     2761 cagcgcgaca tcctcgcgga tgagcagaag caaggcgagc atcgcgtagt agagacggag
     2821 cttgcccgac aggaagaagt acagcgcgaa gagcagcggc gagaacagga gcgtgagcga
     2881 gtggaactcg tacatgttcg cgccgtgaag cgcggggtgt aatgcgtacg cgagcgccaa
     2941 gatgagcccc gatgcgcagg ttccgagccg gtcgcggccg agg
//
LOCUS       DASRZF010000042         7588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3813974, whole genome shotgun sequence.
ACCESSION   DASRZF010000042 DASRZF010000000
VERSION     DASRZF010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 7588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7588
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3813974"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1089
                     /locus_tag="VFG22_01855"
     CDS             <1..1089
                     /locus_tag="VFG22_01855"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HET6415011.1"
                     /translation="LFVRPRKREREIWDGPRAGVEGAVRDFGADAAFPIEELAQRLPD
                     YLGNVERLHYRIATNGQADAKIFDCLNQLRRGGRRGIVAPEVIVDSSVHLHELRLRKS
                     DEELALMRQAAAITREAHLRAMQLANAGVREYEIDAELLHAFRKHGSERPAYESIVGS
                     GPNATILHYRAGNRVMNDGELLLIDAGCEYEYYASDVTRTFPVNGKFTPEQRAIYELV
                     LHAQKASIEAVRPGVTLDDIHDGAVRTITEGLIELGLLSGNLDSLIEEKKYEAFYMHR
                     TSHWLGMDVHDVGHYYRGGKQRSLEAGFVLTVEPGIYIATDSEAVDERWRGIGVRIED
                     DVVVTDSSSEVLTAGIPKEIDELEAACA"
     gene            1141..1974
                     /gene="proC"
                     /locus_tag="VFG22_01860"
     CDS             1141..1974
                     /gene="proC"
                     /locus_tag="VFG22_01860"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HET6415012.1"
                     /translation="MPLEHSIAFVGAGNMAGALIRGLVGTGTVAAGQIIAADPDQARI
                     AALGSELGIRTTTENADAVAAANVVVLATKPQVFAQVLPGVAAAFDPGALLISIAAGI
                     STRVIERAFPPGSRVVRSMPNTPALVGAGATAIAAGAHATEADLELSELLFRSVGIAV
                     RVPEDQIDAVTGLSGSGPAYVFAMVEALRDAGINEGLPEDTALQLASQTVFGAARLLL
                     DEKEAPEVLRARVTSPGGTTRAGLDALASADFAGVVRGAVRAATRRSVELGKIAEGSE
                     D"
     gene            complement(1971..2657)
                     /locus_tag="VFG22_01865"
     CDS             complement(1971..2657)
                     /locus_tag="VFG22_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HET6415013.1"
                     /translation="MSRDLRTAIERVQKRIARACERAGRDVDTVQLISVSKGHPVETI
                     RAAYDAGMRVFGENYAQELAEKASILSDLEGLQWRFIGHLQRNKIKLIERARASVDTV
                     DSLRLAEAISSRAQLDGREVELLIQVNVAREAQKSGCTPDALPELVEAVQALPNVDLR
                     GLMTVAPDLDDPEETRPFFSALRELAKSQGLPELSMGMTHDLEQAVEEGATMLRIGTA
                     IFGPRPAVEP"
     gene            complement(2654..3253)
                     /locus_tag="VFG22_01870"
     CDS             complement(2654..3253)
                     /locus_tag="VFG22_01870"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HET6415014.1"
                     /translation="MTAARGPLVLASASPRRREILSALGLEFEVHPSGADEGGLRIED
                     HLEFVRAAALLKLQTALDVEVREGVFVLAADTIVTVDDQRLGKPVDDLDAVRMLSLLA
                     GRDHLVRTAIALGRAADGVLASRVVETRVWFKPASREALARYVAAGESLDKAGAYGIQ
                     GLASGFVTRLEGSYTNVVGLPASEVISMLLDRGALEQWP"
     gene            complement(3250..4803)
                     /locus_tag="VFG22_01875"
     CDS             complement(3250..4803)
                     /locus_tag="VFG22_01875"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6415015.1"
                     /translation="MPPTTIAILGSKGGVGTSLFSANLAIYLATIGKRVLAVDADPAG
                     ADLNAMLGVGPRVAPYTAPAPAFGVEGSLSSLAAALDHDAINTLPLREPIDGPISSLL
                     LMDGGLSEPHRGSARRSSLRELEARLGKLDADYAVVDLGSAPTDAAIRFWCGAAHRLA
                     LTVPEPNALSGLYRLVRKSFAATALGRVSDERQQVALHAALAKAGQSPAPSDIAALMQ
                     REWPEIGRALQACMSSLDFPFIVNQARLRADLELGDQVTVAVRRRYGVHLKYLGYVEH
                     DDAVRTCSRKRRPLLLESPGSRASRNIEKIARRLFASQEGRRDRAPWREVPPSSHHDV
                     LEVERGATDEEIRRAYKRMCAVFDPENLVTFGLFDEEGLDAARTRIDEAYDVLLDPAR
                     RRPYEISVFPEAEETKAEAEQAETPNGELPPAPSITPDTEFSGALLRAVRESQGTSLE
                     QISERTKVGTSYLRCIEEEVFSQLPASVYVRGFVTEFAKCLRLDPEQVSQSYIRRYKA
                     RMEQKQEDH"
     gene            complement(4856..5071)
                     /locus_tag="VFG22_01880"
     CDS             complement(4856..5071)
                     /locus_tag="VFG22_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415016.1"
                     /translation="MSTQPRMSAPIGRLQIQTSEARKRSLSGTGRWTSAGPPADAELD
                     DSGEAPAEEGRRRYYPFAPSARDDRRD"
     gene            5178..7310
                     /locus_tag="VFG22_01885"
     CDS             5178..7310
                     /locus_tag="VFG22_01885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HET6415017.1"
                     /translation="MGSQESEEVLEGVVEDVLFRSDDGRFAVVRVAPDSGAELAPTMT
                     AVGDLGQIAAGENVRMLGRWTQHAVYGPRFQVLSFTPTIPSSEQGMVRYLGSGLIPGI
                     GPALAARLVERFGPRTLDVIATESARLREVSGIGKQRAESIAKAVRQRRAEAETMSFL
                     HALGMGPATAREIVRKYGADAPRIVRDDPYIVAEQIRGIGFRTADRIAEAIGIAGDDL
                     RRVQGGVLHIIGKAVDEGHVFATKAQLAEGLAQLDVPSQSLEEALQTLRGRELVIIED
                     EAVYPPLLYRAETLVAEALSSLAERPKSKQPVKTFDHNLSDMQRSAVDASLGTHLLVL
                     TGGPGTGKTTTVRAIVQAHAALDRTVALCAPTGRAAKRLSEAAGIEAKTIHRLLEWNP
                     ATAHFNRNRRTPLEADLILVDEASMLDIRLASQLLAAIPATATLVLVGDIDQLPPVGP
                     GQPLRDLIASRICKTIRLHEVFRQAQQSAIVRGAHSVLEGVMPEATPAGTKGDGDLFI
                     IAATDPETITERLLQALRRMSVSYGFDPSADVQVMTPMRRGPLGTHRLNEILQEALNP
                     SPQPNAVPAGFRPGDKVMQLRNDYEKEVYNGDLGEVHHIEGGVTYVRFDGREVQYKGD
                     ELDAISLAYACTVHKVQGSEFPAAVIVLHNAHFMLLNRALLYTALTRAKKLVVLVGDP
                     RAMARAARNALSYETNSRLLDRLRASAA"
     gene            complement(7307..>7588)
                     /locus_tag="VFG22_01890"
     CDS             complement(7307..>7588)
                     /locus_tag="VFG22_01890"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET6415018.1"
                     /translation="EELVRHSTAVRAGGTLRLGWDGKAPEGSAAGTSIDADAFGHLGF
                     TGTSIWCDPRRQIVVVLLSNRVAVSDDNTAIRAFRPGFQDAVIAAFDGR"
BASE COUNT         1395 a         2473 c         2377 g         1343 t
ORIGIN      
        1 ctctttgtcc gcccgcgaaa acgggagagg gagatctggg acgggccaag ggctggggtc
       61 gaaggggcgg tacgagactt cggggccgac gctgctttcc caatcgaaga gttggcccag
      121 cggctccctg actacctcgg caacgtcgag cgtctgcact atagaatcgc gaccaacggg
      181 caagccgacg ccaagatctt cgactgcctg aatcagcttc gccgcggggg gcggcgcggc
      241 atcgtcgccc cggaggtgat cgtcgactct tccgtccacc ttcacgagct gcgactgcga
      301 aagtccgatg aagagctagc gctgatgcgg caggccgcgg cgatcacccg ggaagcacac
      361 cttcgcgcga tgcaactcgc taatgcgggc gtccgtgaat acgaaatcga cgccgagctc
      421 ttacacgcat tccgaaaaca tggcagcgaa aggcccgcgt acgagagcat cgtcgggtcc
      481 ggtcccaacg ctacgatctt gcactatcgg gcgggcaatc gagtcatgaa tgacggggag
      541 ctgctcctca tcgacgcggg gtgtgagtac gagtactacg cgagtgacgt cacgcgcaca
      601 ttccccgtca acggtaaatt cacaccggag caacgcgcga tctacgagct cgtgcttcac
      661 gctcaaaagg caagcatcga agccgtgcgg ccgggggtaa ctctcgacga catccacgat
      721 ggcgcggtcc gaacgatcac cgagggcttg atcgagttgg gactgctgag cggcaacctg
      781 gattcgctca tcgaagagaa gaaatacgaa gcgttctaca tgcatcgcac ttcgcattgg
      841 ctcggcatgg acgttcacga cgtcgggcac tactaccgag gcggcaagca gcgctcgctc
      901 gaggcaggct tcgttctgac ggtcgagcct gggatctaca tcgcgacgga ctccgaagcc
      961 gtggacgaac gctggcgcgg gatcggagtg cgcatcgaag acgacgtcgt cgtcaccgac
     1021 tcgagctccg aggtcctcac cgcagggatt cccaaagaga tcgacgagct cgaagcggcc
     1081 tgcgcgtagc cgccgagcac gacgtgtcgc gtggcgcgcg agtatggtat gccgcgcgtc
     1141 atgcctctcg agcacagcat cgccttcgtc ggcgccggca atatggcggg cgctctcatt
     1201 cgcggtctag tcggaaccgg gaccgtggct gccggccaga tcatcgcggc tgacccggac
     1261 caggcgcgta tcgccgctct cggatcggag ctcggcattc gcacgactac cgagaacgcg
     1321 gacgcggtcg ctgcggcaaa cgtcgtcgtg ctggccacca aaccgcaggt gttcgcccag
     1381 gtgcttcctg gcgtcgctgc ggcattcgac ccgggcgcgc ttctgatatc gatcgccgca
     1441 gggatttcga cgcgcgtcat cgaacgagcc ttcccgcctg gttcgcgcgt ggtgcgttcc
     1501 atgccgaaca cgccagcgct cgtcggagcg ggcgcaacag cgatcgcggc cggcgcgcac
     1561 gcaacggagg cagacctcga gctatcggag ctgcttttca gaagcgtggg catcgccgtg
     1621 cgcgttccag aggatcagat cgacgccgtg accggcctga gcggctcggg cccggcgtac
     1681 gtattcgcga tggtcgaagc gcttcgcgat gcgggcatca acgaggggct tccggaagac
     1741 accgcgctgc agctggcctc gcaaaccgtg ttcggcgcag cgcgattgct tctcgacgaa
     1801 aaggaagcgc ccgaggtctt gcgcgctcgg gtgacgagcc ctggcgggac cacccgggcg
     1861 ggattggacg cgctggcatc ggcggatttc gctggcgtcg ttcgaggcgc cgttcgtgcg
     1921 gcgacgcggc gctcggtgga gctcggcaag atcgccgaag gctccgagga ctagggctcc
     1981 accgctggac gcggcccgaa gatcgccgta ccgattcgaa gcatggtggc cccctcctcg
     2041 acggcttgtt cgaggtcgtg agtcattccc atcgaaagct cgggcaaccc ttgagatttg
     2101 gcgagctcgc gcagcgctga gaagaacggc cgcgtctctt ccggatcatc caggtcaggg
     2161 gcgacggtca tcagtccgcg aagatccacg ttcggcaatg cctgcaccgc ttccacgagc
     2221 tcgggaagag cgtcaggggt gcagcccgat ttctgcgctt cgcgcgccac gttgacctga
     2281 atcaagagct cgacctcgcg cccatcgagc tgcgctctcg aagaaatcgc ctccgccagc
     2341 cgcaatgaat ccaccgtgtc cacgctggcc cgcgcgcgtt cgatcagttt gatcttgttg
     2401 cgctgcaagt gccctatgaa acgccactgg agcccctcga ggtcgctgag aatcgatgcc
     2461 ttctccgcga gctcctgggc gtagttctct ccaaagacgc gcattccggc atcatacgcg
     2521 gctcgaatcg tctccaccgg atgccccttc gaaaccgaaa tgagctgaac agtatcgacg
     2581 tccctgcctg ctcgctcaca agcccgggcg attcgcttct gcacgcgctc gatggcagtg
     2641 cggaggtctc gactcatggc cattgctcca aggcccctcg atcgaggagc atgctgatga
     2701 cctcggaagc gggaaggcct accacgttcg tgtaggatcc ctcgagccgt gtcacgaacc
     2761 cgctcgcaag cccttggatc ccgtacgcgc cggccttgtc gagactctcg ccggctgcca
     2821 cgtagcgcgc caacgcctcc cgagaggcag gtttgaacca gacgcgggtt tcgaccactc
     2881 ggcttgccaa aactccgtca gcggctcgac cgagcgcgat ggccgtccga acgaggtggt
     2941 cacgtcccgc aaggagcgac agcatccgaa cggcgtccag atcgtccact ggttttccca
     3001 gacgctgatc gtcaaccgtg acgatggtgt cggccgcgag aacgaacacg ccctctcgca
     3061 cttcaacatc caatgcggtt tgcagcttga ggagtgcggc tgcccgcacg aactccaaat
     3121 gatcctcgat acgcaagccg ccttcatcgg cgccgcttgg atgaacttcg aactcgaggc
     3181 cgagggccga gaggatctct cgacggcgcg gcgacgccga tgcgaggacg aggggtcccc
     3241 ttgccgcagt caatggtcct cttgcttctg ctccattcgc gccttgtatc gacgtatgta
     3301 gctctggctg acttgctcgg gatcgagcct caggcacttc gcgaactcgg tcacgaagcc
     3361 ccgcacgtag acggaagcag gaagctgcga aaacacttcc tcttcgatgc atcgcagata
     3421 gctcgtgccg accttcgttc tttcgctgat ctgctcgagg ctcgtgcctt gcgactctcg
     3481 aacggctctc agaagcgcgc cactgaactc ggtgtccggg gtgatgctcg gggctggcgg
     3541 cagctcgcca ttcggagtct cggcctgctc agcttcggct ttggtttcct ccgcctctgg
     3601 gaacaccgaa atttcatagg ggcggcgccg ggcaggatcg agaagaacgt cataggcctc
     3661 atcgatccgc gtccgggcag catcgagccc ctcttcatcg aacagcccga aagtgacgag
     3721 gttctcaggg tcgaataccg cgcacatgcg cttgtacgct ctccggatct cttcgtctgt
     3781 ggcgccgcgt tccacctcga ggacgtcgtg atgggagctt ggtggaacct ctcgccacgg
     3841 cgcgcggtct cttcgccctt cctgcgacgc aaagagacgc cgtgcaattt tttcgatgtt
     3901 gcggctcgcc cgactgccgg ggctttcgag gagaagcggc cgccgctttc tcgagcatgt
     3961 tcgaaccgcg tcgtcgtgtt cgacataacc gaggtacttc aggtgaacgc cataacggcg
     4021 acgcaccgca acggtgacct gatcgcccag ctcgaggtcc gctcgtaaac gcgcctggtt
     4081 cacgatgaac gggaagtcca agctgctcat gcaggcttgc aaggcgcggc cgatctctgg
     4141 ccactcgcgc tgcatcagtg ccgcgatgtc cgatggcgcc gggctctgac ctgctttggc
     4201 aagggcggca tgtagggcga cctgctggcg ctcgtctgaa acgcgaccga gcgcggtcgc
     4261 agcaaacgac ttgcgcacga gccggtagag cccgctgagg gcattcggct caggcaccgt
     4321 cagggcaaga cgatgggctg ctccgcacca gaaccgaatc gccgcgtcgg tcggagcgga
     4381 tccgaggtcg accaccgcgt agtccgcatc tagtttgcca agacgagctt cgagctcgcg
     4441 gagtgagctt cgacgcgcgc tccctcggtg aggctcgctg aggcctccgt ccatcagcaa
     4501 gaggctggaa atcggcccgt cgattggctc tcggagggga agcgtgttga tcgcgtcgtg
     4561 gtcgagcgca gcggccagac tggacagact gccctccacc ccgaaggcgg gcgcgggcgc
     4621 cgtgtagggt gcgacgcgag gcccgacgcc gagcatcgcg ttgaggtccg cacccgccgg
     4681 gtctgcatcc accgcgagga cccgcttgcc aatcgtcgcc agatagatcg ccaggttcgc
     4741 gctgaagagc gaagttccga cgcctccctt ggacccgagg atggcgatcg tggtcggcgg
     4801 catcgagcgc tcttaccaat cggtcgatcc tcgcgcaatg acgcgctcgc ctgattcagt
     4861 cgcgacggtc gtcgcgcgca cttggcgcga acgggtaata tcgccgtctg ccctcctccg
     4921 caggggcttc tcccgagtca tccagctcgg cgtcggccgg cggtcccgcc gaggtccagc
     4981 ggcccgtgcc agaaaggctt cgcttccggg cctccgaggt ctgtatctgc agtcttccga
     5041 tgggtgcgct catacggggc tgagtactca tcttcctcca atccaaatcc gtgcgctttt
     5101 aacagtaacg gacgattatg ctgaacgcac aaccgcagag cgttttcaac tcaagactga
     5161 tagattccgc ccgaatcgtg gggtcgcagg agtcagaaga ggtcctcgaa ggcgtcgtgg
     5221 aggatgtact gttccgcagt gatgacgggc gcttcgcggt ggttcgcgtc gctccggaca
     5281 gcggcgccga gctggctccg acgatgacgg ccgtcggcga tctggggcag attgccgctg
     5341 gggaaaacgt tcgcatgctg gggcgctgga cgcagcacgc ggtatacggc ccccgctttc
     5401 aagtgctctc attcacccct accattccct ctagcgagca ggggatggtt cgatacctcg
     5461 gctccggcct gatcccgggc atcgggcccg cactcgccgc gcgcctggtc gagcgtttcg
     5521 ggccgcgcac cctggatgtc atcgccaccg agtccgcccg gcttcgcgag gtctccggca
     5581 ttggcaagca gcgcgcggaa tcgatcgcca aggccgtgcg gcagagacgt gcggaggcgg
     5641 aaacgatgag ctttctgcat gcccttggaa tgggtccggc caccgcccgt gagatcgttc
     5701 gcaagtacgg agcagatgcg ccgcgaatcg tgcgcgacga tccctacatc gtcgccgagc
     5761 agattcgcgg catcggtttt cggactgcag accgcatcgc agaagcgatc gggattgcgg
     5821 gcgacgacct gcgccgcgtg caaggcggcg tgctccacat catcggcaag gcggtcgacg
     5881 aaggccatgt ctttgcgacc aaggcgcaac tcgccgaagg cctcgcacag ctcgatgtgc
     5941 catcgcaatc cctcgaagag gcgctgcaga cgttgcgcgg gcgagagctg gtgatcatcg
     6001 aggacgaagc ggtctaccca ccgctgctct accgcgcgga aaccctcgtg gccgaggctt
     6061 tgagcagctt ggccgagcgc ccgaagtcga agcaacccgt caagactttc gaccacaacc
     6121 tgagcgacat gcaaagaagc gcggtcgacg cctcacttgg aacgcatctc ctcgttctca
     6181 caggcggacc aggcacgggc aagaccacca ccgtgcgcgc gatcgtacaa gcccacgctg
     6241 cactcgatcg aaccgtggcg ctctgcgcgc caaccggacg ggcggcgaaa cgcctgtcgg
     6301 aggctgctgg catcgaagcc aagacgattc accggcttct cgaatggaat ccagcgaccg
     6361 ctcatttcaa tcgcaatcgc cgcacaccgc tcgaggccga cctcatcctc gtggacgaag
     6421 cctcgatgct cgacatccgg ctcgcgtcgc agcttctggc cgcgatcccc gcaacggcaa
     6481 cgctcgtgct ggtcggcgac atcgatcaac tgccacccgt gggtcccggc caacccctgc
     6541 gcgacctcat cgcgtctcga atctgcaaga cgattcgtct gcacgaggtt tttcggcaag
     6601 ctcagcagag cgcgatcgtc cgaggtgccc actcggtgtt ggaaggggtc atgccggaag
     6661 ccacgccggc cggcacgaaa ggcgatggcg acttgttcat catcgccgcc acggatccgg
     6721 agacaatcac cgagcgcctc ctacaggcgc tacgccgaat gtcagtctcc tacggcttcg
     6781 acccatcggc cgatgtgcag gtgatgaccc cgatgcgtcg tggcccactt ggcacgcacc
     6841 ggctgaacga aatcctccaa gaggccctca atccaagtcc tcaacccaac gccgtgcccg
     6901 cagggtttcg gcctggggac aaggtgatgc agcttcggaa cgactatgag aaggaggtct
     6961 acaacggtga cctcggggaa gtccatcaca tcgaaggcgg tgttacctac gttcgtttcg
     7021 acgggcgcga ggtgcaatac aagggcgacg agctcgatgc gatctctctt gcctacgcgt
     7081 gcacggtcca caaggttcaa ggcagcgaat tccctgccgc ggtcatcgtc ctgcacaacg
     7141 cccacttcat gttgttgaac cgcgccctgc tctacacggc actcacccgg gcgaaaaagc
     7201 tcgtcgtttt ggtcggcgac ccacgggcca tggctcgggc cgcccgaaac gcgctctcgt
     7261 acgaaaccaa ctcgaggtta ctcgatcgat tgcgcgcaag cgctgcttag cggccatcga
     7321 atgcggcgat gaccgcatcc tgaaagcctg ggcgaaaagc ccgaatggcc gtgttgtcgt
     7381 ccgatacagc cactcgattg ctgaggagca cgacgacgat ttgtcgccgt ggatcacacc
     7441 aaatcgaagt accggtgaag ccgaggtgcc cgaacgcgtc ggcgtcgatc gacgtgcccg
     7501 cggcgctgcc ctccggggcc ttgccgtccc agccgagcct cagcgtgccg ccggctcgga
     7561 cggccgtaga atgccgaacc agctcctc
//
LOCUS       DASRZF010000043         8461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4503390, whole genome shotgun sequence.
ACCESSION   DASRZF010000043 DASRZF010000000
VERSION     DASRZF010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 8461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8461
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4503390"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="VFG22_01895"
     CDS             <1..775
                     /locus_tag="VFG22_01895"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET6415019.1"
                     /translation="RDYLDALRAYTDTLYGRMLSTSDSSYFLDKTPANALVLPFLQRL
                     YPDAKYVVLTRHPLAVFSSYANSFFNGDWNEAHAFNPILERYVPAMATFLRERPVPLL
                     HLAYEDLVTDPEPQLERVFAFLGLEHDPDAVEYGKAGPQKKGMGDPITVNNEDRPVSD
                     NVGKWALELAHDAGKRALAEQMIDALDAADLEAWQWPAEALWAPVEVAGGGPPPKPAR
                     NSYTFQRRVMLALKKDIHNRPHGKLVKRVKYYCDVLLRE"
     gene            complement(765..1574)
                     /locus_tag="VFG22_01900"
     CDS             complement(765..1574)
                     /locus_tag="VFG22_01900"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6415020.1"
                     /translation="MSKPKRRTARTGSEAHYENGRYYDQAYRLRRHDVRFYSKLALDS
                     GGPVLELGVGTGRVTIAIAEAGIEVVGVDPMTPMLDQARQRLARLPRNTRERVELRRA
                     TLERLRLRRKFPLVIAPFNVWMHLYTREEIERGFATVRHHLAPGGRFAFDVLLPDPVS
                     LARDPAKRYAGGLVPHPRDGVRYRYGEYFSYDPMTQIETVVMDFEHPVNKEQSFCTPL
                     TQRQFFPAELESLLHYNGFQVESHTGDFEGKPIDAGTESQVVIARMRRPTP"
     gene            complement(1567..2346)
                     /locus_tag="VFG22_01905"
     CDS             complement(1567..2346)
                     /locus_tag="VFG22_01905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HET6415021.1"
                     /translation="MESYRELDPTGVRGVVFDIDDTVTRNGVLEPNAYTAMHRLVSAG
                     FELVAVTGRPLGWTDVFARIWPVRVAVGENGAGWTWVDQEGVHEGYFCNETERREQRA
                     LLQTIRDAVSSKMPGVRCTSDDRHRRCDLAFDVGESVTLPRKEIDALVALIEAMGARS
                     SVSTVHAHAVPGAWDKAEGVARALQQVCGVNLEEESDRWVFVGDSGNDAAAFARFPKS
                     VGVANVRAHLDRLPIPPRYLTDADRGLGFSELAMHLIGDRV"
     gene            2426..2662
                     /locus_tag="VFG22_01910"
     CDS             2426..2662
                     /locus_tag="VFG22_01910"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HET6415022.1"
                     /translation="MPNLGFGELVIVFLIVMLIFGAGRLPQIGEGLGKAIRNLKRGLN
                     TDDDIDVTPTEKQVPQKAASRVEDAEVVDKVESK"
     gene            complement(2673..3434)
                     /locus_tag="VFG22_01915"
     CDS             complement(2673..3434)
                     /locus_tag="VFG22_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415023.1"
                     /translation="MGDARSFGDLFRLLASLDELDMRVGLLCERMTSIASEDAVCLLD
                     EAHQRAALGGTEAQRVFLAIGWALFDTRLQERRPELGEAARNAGLHHIADFLTPPAES
                     ESPPEDKRRIPDFGRGRPVTLGERKSLARTHDRALIQRVVRDPHPDVVRILLDNPSLT
                     EEDVVHVCATRPNDPEVLQAVYRHRRWVVRYRPRNAIVRNPDTPLDTALLLVPLLRKP
                     ELREAATSPELAPALRLSCRRILETRSAPPGDGDS"
     gene            3523..4026
                     /locus_tag="VFG22_01920"
     CDS             3523..4026
                     /locus_tag="VFG22_01920"
                     /inference="COORDINATES: protein motif:HMM:NF019494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HET6415024.1"
                     /translation="MAEAQPVRVIKRYANRKLYDTRDSKYITLDRIAELVRNGEEVKV
                     LDNRSKDDLTKLTLAQIIYEEQKSGHATAWSVQSLRGFIQQGRQKFLTSLKEGPVGKL
                     VERAEELRAEVYSAADDRVRSVMESAAAHVRQLQAEVLRLQARIEELESKVRHASSKD
                     EPPDNVE"
     gene            4057..4701
                     /locus_tag="VFG22_01925"
     CDS             4057..4701
                     /locus_tag="VFG22_01925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6415025.1"
                     /translation="MPRKSKKFDTERELFESEARPHLDVLYSIGLRLTRSPVDAEDLV
                     QDTLVRAYRFYDRFEAGTNFKAWLLRIQMNTFVNRYRRATRERQVFEGPMAMPVGEGV
                     MGRATMRGLTDPVGDAQRRLIGREIQRAFDELSEDARAMVLLADVEELSYKEISEIVG
                     CPIGTVMSRLHRARKQLQGSLQSHAVQLGIIEDDEKVDDPIPLEAFRARKLVKS"
     gene            4698..5492
                     /locus_tag="VFG22_01930"
     CDS             4698..5492
                     /locus_tag="VFG22_01930"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET6415026.1"
                     /translation="MTCKLVRRHLGAFVDGETDPATQIEFERHLEACPGCQEHLAFER
                     SLREQTRESLGGIRAPEQLWNRALHRLDEIDVVRKDRRSPIDARPMHLRQTWTIAAAA
                     AAILIIGGVVGLPEGTDYQSAGMLQDVVNLHSQALPSDVKAEAPQEVVRYFQGKTPFP
                     VRPAQFEEPSVKFVGARYIKVGVHPAAALYYNHGGHRVTLLVFQSPEIMRNAQRTNVG
                     GREVYYHNVGGHVVTIRQHGGVNYAFYGDLDRPVLFQLAANARVED"
     gene            5670..7328
                     /locus_tag="VFG22_01935"
     CDS             5670..7328
                     /locus_tag="VFG22_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415027.1"
                     /translation="MSDENMPSDAPTKIGEPPEIDDEIDEFAESAFDALMMEGERKPA
                     SARAESIESHAPAEMQTGEYEDVELDDVIMVSEVPAGELSAASVPPSAPPGSLRPESA
                     PPAPTEGPGTREVEVARLRSEISGLKAKLAENSGGVSSRQFLDLREALNTKDKQILEL
                     RDQVSARDKEIIELRDQSIVLERARADFDEKTAGLERSLSKAQDAIASLTEDKENANK
                     RYEDVKARFERAKAKSEGLEAELSQEREARAADIEALKGTHDAEVAKFRVAHEQAMDL
                     LKAESAAALAKAQEDNEAAMHAAASEKETALASLRSELADAEAAALAQVAAEADASKQ
                     AALASLREELDAAASARMAAAEAEWQRTMAEREAALQGEKQLELETQEQEHSKQLATL
                     GRKLGDTETELAGAREREAMNEERSIELEAKLNETSASLAAANAQLAETREERDRLDA
                     ELGETKEARDGLQQAFDSSQTKVGLLEGNKRDLESRVGSLEAMKSQLESKLTMALEKI
                     ATDEGLLERVRKAMAIGLSLLEEQERNTYGDATSEMEGVSEDAE"
     gene            complement(7288..8289)
                     /locus_tag="VFG22_01940"
     CDS             complement(7288..8289)
                     /locus_tag="VFG22_01940"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6415028.1"
                     /translation="MAPSTPERFGDYELIERLGVGGMAETFVALRRGLAGFEQRVCLK
                     RILPTFVNDADFVEMFLREARLSALLHHGHIARVLDFGIAGGTHFLTLELIEGADLRA
                     VLRHLRRRGESLDPGLTSYVAHALASALDFAHTADDEGHAAGIIHRDVSPSNVLLSNA
                     GEIKLADFGIAKAANLPGSIQSGALKGKIPYMAPEYALGRECSARSDLFSLGVLLYEC
                     LAGYRPFDGENDLQTLERARNGRHRGLREASPAAPAPLADAIESLLAPKPEVRPPHAT
                     AFLEMLNEIPPPPLAQRELGDLVRSARRRDAIPSMPGLASTRAAPTLRPRKRLPSRR"
BASE COUNT         1681 a         2529 c         2756 g         1495 t
ORIGIN      
        1 acgggactat ctcgatgcgc ttcgcgcata caccgatacg ctctatggcc gcatgctgtc
       61 cacgagcgac agcagctatt ttctcgataa gaccccagcc aacgccctcg tgcttccgtt
      121 cctgcagcgg ctttatcctg atgcaaagta cgtcgtgctc acacggcacc cgcttgcggt
      181 gttcagctcc tacgcgaaca gcttcttcaa cggagattgg aatgaagccc atgcgttcaa
      241 cccgattctc gagcgctacg tgcccgccat ggcgacgttc ctccgggagc ggccagtccc
      301 cctgctgcac ctcgcctacg aagatctggt gaccgatcca gagcctcagc tagagcgtgt
      361 cttcgctttc ctcgggctcg aacacgatcc ggatgccgtg gagtacggca aggccggtcc
      421 tcagaagaag ggaatgggcg atccgatcac cgtcaacaac gaggaccgcc cagtatccga
      481 caacgtgggg aagtgggcac tcgaattggc gcacgacgca ggcaagcgag cgctcgccga
      541 gcagatgatc gacgcactcg atgcggcaga cctcgaagct tggcaatggc ctgccgaagc
      601 gctctgggct ccggtcgagg tcgccggcgg cggacccccg cccaagccag ctcgcaatag
      661 ctacaccttt caacgtcgag tcatgctcgc cctcaagaaa gacatccaca atcgccctca
      721 cggcaagctc gtgaagcggg tgaagtatta ttgcgacgtc ttgctcaggg agtaggtcgc
      781 cgcattcgag cgatcacgac ttgggactcg gtgcctgcgt cgataggctt cccttcgaag
      841 tctccggtat gcgactcgac ttggaatccg ttgtaatgaa ggagtgattc cagctcggct
      901 gggaagaact gccgctgcgt tagcggcgtg cagaacgact gctctttgtt cacgggatgc
      961 tcgaagtcca tcactacggt ttcgatctgt gtcatcgggt cgtagctgaa gtattcgcca
     1021 taccgatagc ggactccatc gcgcggatgg ggaacgagtc caccggcata tcgtttcgcc
     1081 ggatcgcgcg cgaggctcac cgggtcaggt aacaggacat cgaacgcaaa acgaccccca
     1141 ggtgcgagat ggtggcgaac ggtagcgaat ccgcgttcga tttcctcgcg cgtgtagagg
     1201 tgcatccaga cgttgaaggg tgcgatgacc aaggggaact tccggcggag gcgtagacgc
     1261 tctagggtcg cccgacgaag ctctacgcgt tctcgtgtgt ttcggggtag gcgggcgagg
     1321 cgttggcggg cctggtcgag catcggagtc atgggatcga ctccgacgac ttcgatgccg
     1381 gcttccgcaa ttgcgattgt cacgcgcccc gttccgactc cgagctcgag cactgggcca
     1441 cccgaatcga gggccagctt cgagtaaaaa cgcacatcat ggcggcgcag gcgataggcc
     1501 tggtcgtagt agcgaccatt ctcgtagtgg gcctccgaac cggtcctggc ggttcgtcgc
     1561 ttcggcttag acacggtcgc ctatgagatg catcgcaagc tctgagaatc ccaggccgcg
     1621 gtcggcatcc gttagatagc ggggtggaat gggaagtcgg tcgagatgcg cgcgtacgtt
     1681 cgcgacgcca acgctctttg ggaagcgtgc aaaagcggcc gcgtcgtttc cgctgtcccc
     1741 aacgaagacc catcgatcgg attcctcctc gagattgact ccgcacacct gctgcaatgc
     1801 gcgcgccacg ccttcggctt tgtcccacgc tccggggacc gcatgggcat gaacggtgga
     1861 caccgagctt cgagcaccca tcgcttcgat cagcgcgacc aaagcgtcga tctctttgcg
     1921 cggcagggtc accgattcgc ctacgtcgaa agcgaggtcg cagcgacgat gccggtcgtc
     1981 gctcgtgcat cggacccctg gcatcttcga cgaaacggca tctcggatcg tttggaggag
     2041 ggcacgttgc tcgcgtcgct cagtttcgtt gcagaagtag ccctcgtgta caccttcctg
     2101 gtcgacccag gtccacccgg cgccattctc cccgaccgcg acccgtaccg gccagatgcg
     2161 cgcgaacacg tcggtccagc caagcggccg cccagtgacg gccaccagct cgaatccggc
     2221 gctgaccagg cggtgcatcg cggtgtacgc atttggctcg aggaccccat tccgggtcac
     2281 cgtgtcgtcg atatcgaaga cgacccctcg cacgccggtc ggatccagct ctcgatagct
     2341 ctccatctcg gctcccccat accatacccc tgggcttgcg gaccggttcg gagcggcgta
     2401 ccgtgggaat agcggagggg attacgtgcc caatctcggt tttggcgaac tagtcatcgt
     2461 ttttctgatc gtgatgctga tcttcggcgc cggacgcttg ccgcaaatcg gagaggggct
     2521 tggaaaggcc atccgaaatc tcaagcgggg gctcaacacc gatgacgaca tcgatgtcac
     2581 cccgaccgag aagcaggtcc cgcagaaggc ggcctcccgg gtggaggacg ccgaggtcgt
     2641 ggacaaggtc gagtcgaaat agcgctcggc tcctagctgt ccccgtcccc tggaggcgct
     2701 gagcgcgtct cgagaatccg cctgcagctc aagcgcagag ccggtgcaag ctctggcgac
     2761 gttgcagctt ccctcagctc gggttttcgg agtaggggca ccagcagaag cgcggtgtcg
     2821 aggggagtat cggggttgcg gacgatcgcg ttccgggggc ggtatcgcac cacccagcgc
     2881 cggtgacggt acaccgcctg aagcacttcg ggatcgttgg gtcgggtagc gcagacatga
     2941 acgacgtcct cttcggtgag ggaagggttg tcgagcaaga tccgaacgac gtcggggtgg
     3001 gggtcgcgga cgacgcgttg aatcaaggcg cggtcgtggg tccgggcgag tgacttgcgt
     3061 tcgccgagcg tgacgggtcg cccgcgacca aagtcgggaa tgcgccgctt gtcttcgggc
     3121 ggcgactcgg actcggccgg cggggtgagg aagtctgcga tgtgatgcag tccggcgttc
     3181 ctagcagcct caccaagctc cgggcgcctt tcttggagcc gggtgtcgaa gagcgcccag
     3241 ccgatcgcga gaaacacccg ttgcgcctcc gttccgccaa gcgctgcgcg ctggtgagcc
     3301 tcgtcgagca agcagacggc atcttcgctc gcgatcgaag tcatccgttc gcagagcagg
     3361 cccacgcgca tgtcgagctc gtcgaggctc gccaggagcc gaaacaggtc tccaaaggat
     3421 cttgcgtcgc ccacagcctg actgccccta tgaccagcct aatgagaagg ccccgaagtg
     3481 ccagcttgac gcagtgcgtc gccaggctag cctcgtttca gaatggctga ggcgcaaccg
     3541 gttcgggtga tcaagcggta cgcaaaccgc aagctctacg acacccgcga tagcaaatac
     3601 atcaccttgg accgcattgc ggagctcgtt cgcaacggtg aagaggtgaa ggtgctcgac
     3661 aatcgttcga aggacgacct caccaagctc acgctcgcgc agatcatcta cgaggagcaa
     3721 aagagcggcc acgccacggc atggtccgtt cagagcttga ggggcttcat ccagcagggc
     3781 cgccagaagt tcctgacctc gctcaaggaa gggccggtcg gaaagctcgt agaacgtgcc
     3841 gaggagcttc gagcagaggt gtacagcgcc gccgacgacc gtgttcgatc ggtcatggaa
     3901 agtgcggcag ctcacgtaag gcagcttcag gctgaggtgc tccggcttca ggctcgaatc
     3961 gaagagctcg agtccaaggt ccgtcacgcc tcctcaaaag acgagccgcc tgataacgtg
     4021 gaataatccc cgttttcact ggttctaaaa gcaggcatgc ccagaaaatc caagaaattc
     4081 gataccgagc gcgagctttt cgaatcggag gcgcgccccc acctcgatgt gctttattcg
     4141 atcggactcc ggctcactcg ttcgccggtc gacgccgagg acctggtgca agataccctg
     4201 gttcgcgcct accgattcta cgatcggttt gaggccggga cgaatttcaa agcctggctt
     4261 ctgagaatcc agatgaacac gttcgtcaat cggtaccggc gcgcgacccg tgagcgtcag
     4321 gtctttgagg gtccgatggc gatgccggtt ggagagggcg tcatgggccg cgcaaccatg
     4381 cgaggattga ccgaccccgt gggcgatgcg caacgccggt tgatcggtcg cgagattcag
     4441 cgcgctttcg atgagctctc ggaggacgcc agggcgatgg tcttgctcgc ggatgtcgag
     4501 gagctctcct acaaagagat ctccgagatc gtgggctgcc cgatcggaac agtcatgtcg
     4561 cgtttgcacc gcgcgcgaaa gcagctccaa ggctccctgc aaagccatgc cgtgcagctc
     4621 ggcataatcg aggatgatga gaaggtcgac gacccgattc cgctcgaagc gtttcgcgcg
     4681 aggaagttgg tgaagtcgtg acttgcaagc tcgtcagacg tcatctgggt gcgttcgtgg
     4741 atggggagac cgatcccgcg acgcagatcg aattcgagcg ccacctcgaa gcctgtcccg
     4801 gctgccagga gcatcttgcg tttgagcgct ccttgcgcga gcaaacccgc gaatcgcttg
     4861 gcggcattcg cgcgccagaa cagctttgga accgcgcgct gcaccgtttg gacgagatcg
     4921 acgtggtcag gaaggatcgc cgctcgccga ttgacgcgcg cccgatgcat ctgcggcaaa
     4981 cctggaccat cgcggctgcg gcggctgcga tcctgatcat tggaggggtc gtgggcttgc
     5041 ccgagggcac cgattatcag agcgccggga tgctccaaga tgtggtgaac cttcattcgc
     5101 aggccttgcc gtcggacgtc aaagccgagg cccctcaaga agtcgttcgc tactttcagg
     5161 gcaagacccc gttcccggtc aggccggctc agtttgaaga gcccagcgtg aagttcgtcg
     5221 gcgcgcggta catcaaagtc ggtgtccacc cggccgcagc gctctactac aaccacggcg
     5281 gccaccgcgt gactctcctg gtcttccagt ctccggagat catgcgaaac gcacagcgaa
     5341 ccaatgtcgg tggccgtgag gtctactatc acaatgtagg tggtcacgtg gtgaccatcc
     5401 gccagcatgg tggcgtgaac tatgcgtttt atggcgacct cgaccgtccg gtgctgttcc
     5461 agctcgcagc caacgcccgg gtcgaggact agcgttcccc actccgctgg tcttttgcgg
     5521 gggttcagcc gttgacaggt cggcgccgcg acgcctattc ttttttgacg taggcgtaag
     5581 tttgcatgcg ctgaaggggt tggaagcagt ttcgttccta tcgacgagat cgctggtaac
     5641 ctggctgtgg gaatcgaagg ggagagccaa tgtcggatga aaacatgccg agcgacgcgc
     5701 caaccaagat cggggagcca cccgagattg atgacgaaat cgacgagttt gccgagagtg
     5761 cgttcgacgc gctcatgatg gaaggcgagc ggaaaccggc ctcggcacgc gccgaatcga
     5821 tcgagtccca cgcgccggcc gagatgcaga ccggcgagta tgaggatgtc gagctcgatg
     5881 acgtgatcat ggtgtcggag gtccctgccg gggagctctc cgccgcctcg gtgccgccct
     5941 ctgcgccgcc cggttccttg cgcccggagt ccgctccgcc ggccccgact gagggcccag
     6001 gcacgcggga ggtcgaggtc gctcgactgc gttccgaaat ctccggactg aaggcgaagc
     6061 tcgccgaaaa ttctggcggc gtatcgagcc gccagtttct cgatctccgg gaagcgctca
     6121 ataccaagga caaacagatc ctggagctgc gggatcaggt cagcgcccga gacaaagaga
     6181 tcatcgaact gagggatcag tcgatcgtcc tcgagcgcgc gcgcgccgat ttcgacgaga
     6241 agaccgcggg gctcgagcgc tcgctctcga aagcgcaaga cgcgatcgcg agtctgacgg
     6301 aagacaaaga gaacgcgaac aagaggtacg aggacgtcaa ggctcgattc gaacgggcga
     6361 aagcgaagtc tgaagggctc gaagcggagc tttcgcaaga acgggaagct cgtgcagcgg
     6421 acatcgaagc gctcaaagga acgcacgacg ccgaggtggc gaagttccga gttgcgcatg
     6481 agcaggcaat ggacctgttg aaggcggagt ccgccgctgc gctcgcgaag gctcaagaag
     6541 acaacgaagc tgcgatgcat gcggcggcga gcgagaaaga aaccgcgctc gcttcgcttc
     6601 gctcggagct ggccgatgcc gaagcggctg ctctggccca agtcgctgcg gaggcggatg
     6661 cctcgaagca ggcggcactt gcatcgcttc gagaagagct cgacgccgcc gcgtcggcgc
     6721 ggatggccgc agccgaggca gagtggcagc gcacgatggc ggagcgcgag gctgcgcttc
     6781 agggcgagaa gcaactcgag ctcgagacac aggaacaaga acactcgaag caactcgcga
     6841 cccttgggcg caagctcggc gataccgaga cagagcttgc aggcgctcgc gagcgcgaag
     6901 cgatgaacga ggagcgctcc atcgagctcg aggcgaagct caacgagacg agcgcaagtt
     6961 tggcagcggc caatgcgcag ctcgcggaaa ctcgcgaaga acgagaccgc ctcgatgcgg
     7021 agcttggtga aaccaaggaa gcccgcgatg gcttgcaaca ggcgttcgat tcgagtcaaa
     7081 ccaaggtggg tctactcgag ggcaacaagc gggacctcga gtctcgcgtg ggatcactcg
     7141 aagcgatgaa gtcgcaactc gaatccaagc tcacgatggc gctcgagaag atcgccaccg
     7201 acgaagggtt actcgagcgc gttcgcaaag ccatggccat cggcctcagc ctgctcgaag
     7261 aacaagaacg gaatacctat ggagatgcta cctccgagat ggaaggcgtt tccgaggacg
     7321 cagagtaggg gccgcgcgag tcgacgccag cccgggcatg ctcggaatgg cgtcgcgcct
     7381 tcttgccgaa cgaacgaggt cgcccagctc acgctgcgcg agcggtgggg gcgggatttc
     7441 gttcagcatc tcgaggaatg ctgtcgcatg tggtgggcgc acctcgggct tcggagccaa
     7501 gagtgattcg atggcgtcgg ctagtggagc gggtgcagcc ggcgaagctt cgcgaaggcc
     7561 ccggtgacgg ccattcctgg cccgctctag cgtttgcaga tcgttttcgc cgtcgaaggg
     7621 tcggtatcca gccaggcact cgtatagaag aacgcctaac gaaaacagat cggaccgggc
     7681 gctgcactct cgtccgagcg catactcggg cgccatgtag gggatctttc ccttgagcgc
     7741 gccgctttgg atgctgccgg ggaggttcgc ggctttggcg atgccgaagt cggcgagctt
     7801 gatctcgccc gcgttgctca gaagcacgtt cgagggcgag acgtcgcggt ggatgattcc
     7861 ggcggcgtgg ccttcgtcgt ccgctgtgtg cgcgaagtcg agcgcggacg cgagcgcgtg
     7921 tgcgacgtac gacgtgaggc cagggtcgag cgattctccg cgtctgcgca gatggcgcag
     7981 caccgcacgg aggtcggcgc cttcgatgag ctcgagcgtg agaaagtgcg tcccgccggc
     8041 gatgccaaag tcgaggacgc gggcgatgtg tccgtgatgc aggagcgcgg acagacgcgc
     8101 ttcgcggagg aacatctcga cgaagtcggc gtcgttcacg aacgtgggga ggatgcgctt
     8161 cagacacacg cgctgctcga agcctgcgag gccgcgacga agcgcgacga aggtttcggc
     8221 catgccgccg acgccgaggc gttcgatcag ctcgtagtcg ccgaagcgtt cgggggtcga
     8281 cggagccatg gggaatgcaa aataccatag gccccgcggg aacgtgctgg agtcgaacga
     8341 cttcgtgcgc gagcgcacca aggcagagct caacatggct cagtgtcgcc gatttcgggg
     8401 ccatgattgg cgagcagtcg acggggtctg agaaggcaca cccacatgac catgaccgct
     8461 g
//
LOCUS       DASRZF010000044        15576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_374857, whole genome shotgun sequence.
ACCESSION   DASRZF010000044 DASRZF010000000
VERSION     DASRZF010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 15576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15576
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374857"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VFG22_01945"
     CDS             <1..192
                     /locus_tag="VFG22_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415029.1"
                     /translation="PPLDRREFTVTHNGQCHRYFLTPKEEAVVEALTCRVTRAMQNVI
                     GKAAMAMVEALDVENTLTD"
     gene            329..622
                     /locus_tag="VFG22_01950"
     CDS             329..622
                     /locus_tag="VFG22_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415030.1"
                     /translation="MTIPTEADPRRVPEHERTIVPPKFGLCLCQHRTRTGGLCNAPAM
                     IVLAATPLCRLHERLYSVEARKDEFVAGTNGRDEVRVVLVRLPVTEIAATSLA"
     gene            719..1360
                     /locus_tag="VFG22_01955"
     CDS             719..1360
                     /locus_tag="VFG22_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415031.1"
                     /translation="MATKKVTYVTLPPEHQKTIRGMLLAITAEGRKTMEAPEGVGAKV
                     AKALQDNATTSASTNKALRKLAEAIIGVELPDLANAPTGPEVCSAYVALWNPAGDNYP
                     YIRKDGDTPVPIVRWTNATETGCAFPAGKIADDKWASRFEVGNTPSFSSCGNTYDGRQ
                     VWRYATDAEIDAMDLSVLPSCLAGRAFAEFAPAPIADFPTDDDDDNMPTDDDD"
     gene            1464..1751
                     /locus_tag="VFG22_01960"
     CDS             1464..1751
                     /locus_tag="VFG22_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415032.1"
                     /translation="MPEQIIFGSEGRGERRCAIAWINHRFAGNASMALKARNTFVQIA
                     AERGWGFADASVPHNNDSQKPLGETGQQRIANALHETFERLGIPLNGKEII"
     gene            1921..2262
                     /locus_tag="VFG22_01965"
     CDS             1921..2262
                     /locus_tag="VFG22_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415033.1"
                     /translation="MQQRHRLDRTGEKPNLSAHWPGSAGYLYCGHCHKPMERPGVSYC
                     SIVCAKAESALRAKGEALLRTSEAMANNERAARSAGALREDGSVRTIWDDDALDGPSL
                     ICPPPKDKGNG"
     gene            2271..2534
                     /locus_tag="VFG22_01970"
     CDS             2271..2534
                     /locus_tag="VFG22_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415034.1"
                     /translation="MALVVYTDWPEHSVEVFRLAGHARFEEAVIGFGGILLPDRHRPC
                     FCEVFYSEGGEEGAIAFAIEDYKNALEAGKRPFWPDPSQENKQ"
     gene            2531..2917
                     /locus_tag="VFG22_01975"
     CDS             2531..2917
                     /locus_tag="VFG22_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415035.1"
                     /translation="MRCPYCTSVQVEGEAVAVFTAGTEQTCTCTRCAGTWTDVYAWTH
                     VVRTHIPSLAPRRADSIQCPACLSTNITAFDSITQRYTVADDRTDVLMLNYAGTCDDS
                     ASEVWLECDDCHEEWPIPSNIEVDYT"
     gene            2978..3208
                     /locus_tag="VFG22_01980"
     CDS             2978..3208
                     /locus_tag="VFG22_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415036.1"
                     /translation="MIFTDSQALAHLRESHVRTIEIGRTWRNRNVALKHEHEFDMLIH
                     HEDGDYSGAWIVYSKLNGAYRKKRETPQRPVT"
     gene            3219..3446
                     /locus_tag="VFG22_01985"
     CDS             3219..3446
                     /locus_tag="VFG22_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415037.1"
                     /translation="MSYGIYMNTARVRLSRGDFDGARHACDLAIDEANRILDRALAMS
                     NEIAFERRQAPREKGLNAILADKPADIPGVE"
     gene            3443..3616
                     /locus_tag="VFG22_01990"
     CDS             3443..3616
                     /locus_tag="VFG22_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415038.1"
                     /translation="MIDIAARIVIDHPLLALAIAALLGYAMATLIDGLAFRRQARAMR
                     NLRHKIRMLELRL"
     gene            3613..3930
                     /locus_tag="VFG22_01995"
     CDS             3613..3930
                     /locus_tag="VFG22_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415039.1"
                     /translation="MSIQHEPTEWDGAKAVRERVLIFATRHRLNGSKLLPTFRAIAIE
                     YEAMRRAHHIDQSIIRDLFLERHRVERLLHRHGPDSAIAMLRAVMPHATESQIRRLLE
                     HSR"
     gene            3927..4397
                     /locus_tag="VFG22_02000"
     CDS             3927..4397
                     /locus_tag="VFG22_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415040.1"
                     /translation="MNIVETMAQLQGEIAEAQAKVNAAVNDGLKACERIIEIRDELLL
                     VQRHGRRSIRVVKRRGPYKPKATPVDATSNGHGGTRRAKTDRTVTAIDLLTFLSDGEK
                     TTKAVKAYYGPKRWQTAQSQMGNQRQFRHTSRSNGIWAITKKGAEYLEEKSVNA"
     gene            4390..4704
                     /locus_tag="VFG22_02005"
     CDS             4390..4704
                     /locus_tag="VFG22_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415041.1"
                     /translation="MPDANNSHRSRVLLETDFSFLEREDEKTKAKEKTVHVPEFWRVG
                     GRFHSAKYRFLQHCRRMESFANAIDKEGIPAGMVNRLDRAVTLVQEAALNSTGKHPGK
                     KK"
     gene            4910..5608
                     /locus_tag="VFG22_02010"
     CDS             4910..5608
                     /locus_tag="VFG22_02010"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HET6415042.1"
                     /translation="MTETQSTPSRAIVYARFSPRRNEDETESIETQVEACEDLCRKQG
                     YAVEAVFSDRAASGKDEDRPGLWDAVRSLKKGYVLVVYKSDRLARNLYLSEWLRREIR
                     AKGAKLEVVSGMSEGDSPEATLIRQVLDAFSEYERKLTSARTSFAMLRHQRTGRRMSR
                     HPPYGWDFDPSDPQRWIRNEEEGKAVEKILDWRRKGAAWRRIATLLGEEGHKSRGSEW
                     QGKTVKKIFNRETT"
     gene            complement(5605..5931)
                     /locus_tag="VFG22_02015"
     CDS             complement(5605..5931)
                     /locus_tag="VFG22_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415043.1"
                     /translation="MQSWYTEADVTALSLVSATPVKMGAYSITNVSGGTTFVQFFDAA
                     ATSDVTLGTTLPAWQQFVGSGLANEAHFTSSGLLFKNGLVIAATTASHGGTEEAAGVD
                     ISIGYD"
     gene            complement(5931..6125)
                     /locus_tag="VFG22_02020"
     CDS             complement(5931..6125)
                     /locus_tag="VFG22_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415044.1"
                     /translation="MNDLLKAFSGSKTYILALAGIALLMWGNSAPESWIGLHLDQILA
                     ALGFGTAAALRAGVQKIGVR"
     gene            complement(6122..6631)
                     /locus_tag="VFG22_02025"
     CDS             complement(6122..6631)
                     /locus_tag="VFG22_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415045.1"
                     /translation="MVNIDADRSRVLHLVWVIAIVLGLVCLLGVLVGGCVLPPSSADL
                     AEANRLEQIVDSQQSRIIDQQRQIEALLAKLEAPDTTPEEFTESQTKLAKLIAEKEAL
                     EAEITAQGEQIADLRDGREVEPGEVIMDTAGSVAPFLPPPFGQITAGVLSLVTLIFAG
                     KQIKDRNTP"
     gene            6630..6764
                     /locus_tag="VFG22_02030"
     CDS             6630..6764
                     /locus_tag="VFG22_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415046.1"
                     /translation="MVLPRLLDRDVVNDRHLQLWIQFMEFPPSCALVPPVPYLRLRSL
                     "
     gene            complement(7028..7393)
                     /locus_tag="VFG22_02035"
     CDS             complement(7028..7393)
                     /locus_tag="VFG22_02035"
                     /inference="COORDINATES: protein motif:HMM:NF018101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HET6415047.1"
                     /translation="MRKFGRSKYGVDQTAEGRARRTYQGRVYDSLDERRHAEQLDLDP
                     DVVWWLRQPRFDLGEDDRYAADFLVVERNGTIYAVDVKGVETAGFRRTRKLWAKYADI
                     PLRVVRKGVTIDTVLPPGV"
     gene            complement(7570..7797)
                     /locus_tag="VFG22_02040"
     CDS             complement(7570..7797)
                     /locus_tag="VFG22_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415048.1"
                     /translation="MRKTTPFNPALRGEARNACCFCGHPAPADECLFLHVVDGEETLA
                     CRECIQARHPERRFGRITPHVSRGDVAGFKE"
     gene            complement(8185..8688)
                     /locus_tag="VFG22_02045"
     CDS             complement(8185..8688)
                     /locus_tag="VFG22_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415049.1"
                     /translation="MISQALGWVKRKAMSRMDSTQLRELRELRAYARDMEPILDNIDM
                     LLKLTECEDEVSVPLATSDFWHPDYQSSAKDGPRRFLLSQPVPMLTPHAELHRPVQSY
                     QAMVSHIMYTASIDHRELLLRRTPHEMRCLTDRLTSQIREGLFMHLAECATDPVKRAH
                     AIRVSSG"
     gene            complement(8685..8954)
                     /locus_tag="VFG22_02050"
     CDS             complement(8685..8954)
                     /locus_tag="VFG22_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415050.1"
                     /translation="MDTRETFFPKADPSAERRSRFGLECAKVERERVLVDELLGGLSE
                     EELTALRAATLAQPGIPAFLQDLYPEDSRMLRMLMFNVHQKGELA"
     gene            complement(8954..9451)
                     /locus_tag="VFG22_02055"
     CDS             complement(8954..9451)
                     /locus_tag="VFG22_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415051.1"
                     /translation="MNIGDTYTSGEWLKAADLNGQDYALTIRTVIGEHQFDDGQKQIV
                     IDFHETEKKLGLNWTNANTIAEDLQCGGDDIEQHWPGKKIRVYPTRTQMGKKTVSCIR
                     IRHDWGGNLAPAAPVATQPVAPPVTTQPPTPPFRQDPLTGALNAQPAIGSQKHAPVAE
                     EDIPF"
     gene            complement(9448..9864)
                     /locus_tag="VFG22_02060"
     CDS             complement(9448..9864)
                     /locus_tag="VFG22_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415052.1"
                     /translation="MTPDEILERRSAVLRAVALVKEEAAAFDAAMIEWMNEHRVVSLE
                     MGDKIVTVTRPKKTRCLSSRKAASAVFSASGGDVEALLSCLGANAIKPGAARTILSPD
                     VWDENFEVTVDEKLKIAEIPKGLLKGRCKGSTGDSK"
     gene            complement(9851..10444)
                     /locus_tag="VFG22_02065"
     CDS             complement(9851..10444)
                     /locus_tag="VFG22_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415053.1"
                     /translation="MAESEAFIWDKVSRQKWGTPEKPQGLARDELSAASCLVGIGCLQ
                     RIASAVEILTDPEEAEARRQRSERLGDAGAMVRKVALLKEPVALAVRKKLLEVLKPGY
                     AGRNAQGTFRAKVTRRMLLNVETEADRDRVLAEIAKIDLDWCARTADEILTTAPKAFP
                     AIHGMIDAQRQAKREPTDREPVGAPAHTPVRSGDDTG"
     gene            complement(10743..15551)
                     /locus_tag="VFG22_02070"
     CDS             complement(10743..15551)
                     /locus_tag="VFG22_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415054.1"
                     /translation="MTEAQLRSLSDLMETLPPGTVAALVNDVKNPIDIARPQLVVVVG
                     YERTKGAINPVAGSHWKVIVAAPEGWREQGIFVSRIVPLAGEPRTTLQKSDIPGAIRP
                     YSVAAKQKFTRERILDVFQWAATDAREIRFMATGNLLAAAEQVNNRGQIIMFTTSEGK
                     VRQGILMPPSFTPNEFLNARPIKFSSVENIFKMMEARKPVFADEEGDMIVSLDGGGQT
                     VRFNAVQAKRRGGKYYLHEKALAAAAPNQFVSGGGRMNMRVNLEQAKKVFEAYRASGI
                     FFRPENTPGDRDIAARIEGIDSDVDSAFGGGGGLDKLPGRANIQPQGPSLKAFWPHVP
                     YGEMNPLDDHLPSYIHPREGFLVDAWEATRLLLGEVGSNELRGAVMPKILGAKIMGLA
                     AMRSMPGQSFIHVRDPRVLGTLTHEGMGHGLDFVLLGGKRRFHKTIKHRVPGIGIGEK
                     ALRRELTAASENFRPLPLGMTWQSNNKHIHYRLQNEELVADTLALYAIDPAAAEEKAP
                     EFIANVKEQIAANAFARAIFNMLHNGISVGPTPPVSRSEQHTHIPPPLVHGPLALEPA
                     LRQQAIADARATKNLDAGRTARAYTFTRKLRRMFPETVRMDMHAAIEGTRNVFVDADG
                     NFLKVKGASGIPLQPKAGQPSVVGVTVPGGYYDIAPAGNQDSYEKVMERLQANPNWPL
                     IDRLLREARFMHEEARMDVNKIAEISGKGSEFLSWLPNYVGHEYQVPDADASNFAAKW
                     ITKTKHSKKRKYPTLADAVDDPDFPLRPLTNDLAFTMERWYERNYRAAATLKFVHDLR
                     SLRTADGRKLIVHSHSDPDYVGSGEHQYVKVDIQALNRFFGTVGKDGVFRLFEGNTYV
                     HPEIVRPLQAVLQEPMAEGTLRVINAINQYAKALNVSYSLFHHATLYESSTALNARPE
                     RGQVLRGMLIMPTEQHSLGIARGQFPGERGGIKSFGPMFTATAGHRLAELYGLEDSAS
                     AGLPLDMPASTDHARQYIQKLSRSVSAGTKGIPLVGRLAKLNERMLEAYNRHLWEDVH
                     AGLVLFSYHSLVNQWTGRTTKDGVVVYEKKDQLARRGMSIEDARATAAHVVRNALGGQ
                     RELNLPRFGGDSGFAWAEPITIRQHQWIAQVVFAPDWTIAQIRQAASLPLETSRFVRR
                     NLPQLIPLDFGLMDIPVVGKKLRDVGLQEGQDIIRALTRVRVPIRIERKTSSRLSAGK
                     STEANRGGPGPQEVNNFYAFRATLRIWSNMFGVLVGMMAAAQHAIYLWARDEDPDMQP
                     FPWLNEPGHEFDIDMTPFYRWFHRFWGIPIGLERFYMSPGKQVKEIFRYFDDFPKGLI
                     ESAGNKAARLLQIIGEQAYGHSAGSEFPMAWTGTGFRLRTRGLENMGQRGLHILSRFA
                     PFSLTGNTAFFTFPKRQGMTPWQAREQYTDLMRAWAEPGWWKRTKELRGISKEEQEKA
                     VIDDLRRVDLALKLNGRVGRRDIFAQAKGRLKSAYLNRLIKEMERADDSTDTLVALMN
                     LGATTQYLREAIAGKGGDEKSDAMAKKAEEDLQFVLDAGVRSRILDYDRLMLEGDPAS
                     IAAYDREQRRLELTHRINQLKAGSPEMRERERALDDLIKRRKAGIGDPNQ"
BASE COUNT         2898 a         4956 c         4792 g         2930 t
ORIGIN      
        1 ccgcccctcg accgtcgcga gttcacggtc acccacaatg gccagtgtca ccgctacttc
       61 ctgacgccga aggaggaagc ggttgtggag gccctcacct gccgcgtgac gcgggccatg
      121 cagaacgtca tcggcaaggc cgcaatggcc atggtcgagg ctctggacgt tgagaacacc
      181 ctgaccgact gatccccccc gtcaacccaa acaagcgcga gcaggtagcg aatagcgctc
      241 aagctcaaga ctcaccgctt gttcgtcggg gggacacaag tgccccccgg cgatttcgat
      301 tcaccctctg cagcaggaga cccacccgat gacgatccca acggaagctg acccccgccg
      361 cgtgcctgag cacgaacgca cgatcgtgcc gcccaagttc ggactgtgcc tgtgccagca
      421 ccggacccgc accggcggct tgtgcaacgc cccggccatg atcgtccttg cggctacgcc
      481 cctctgccgt ctgcacgaac gactctacag cgtggaagca cgcaaggacg aattcgtcgc
      541 ggggacgaac ggtcgcgacg aggtacgtgt cgtcctcgtt cggctcccgg tcaccgagat
      601 cgcggctacg tccctcgcgt aattcgaccc gcgccgagcg ctcccatttg ggggcgctca
      661 tcatgggccg cattgtgcaa ccctgtttct tgttccccgc atttggagat caaccatcat
      721 ggctacgaag aaagtcacgt acgtcacctt gccccccgaa caccagaaga ccatccgggg
      781 aatgctcctc gcgatcaccg ccgaaggtcg taagaccatg gaagcgcccg agggtgttgg
      841 ggcgaaagtc gcgaaggcgc tacaggacaa cgccacgacg agcgccagca cgaacaaggc
      901 cctgcgcaag ctggccgagg ccatcatcgg cgtcgagttg cccgatctgg cgaacgcgcc
      961 cacgggaccg gaagtctgct ccgcatacgt tgccctgtgg aacccggcag gggacaacta
     1021 cccctacatc cgcaaggacg gcgacactcc ggtcccgatc gtccgatgga cgaacgcaac
     1081 cgagaccggc tgcgcctttc ctgctggaaa gatcgcggac gacaagtggg cctcccgctt
     1141 cgaggtcggc aacaccccct cgttctcatc ctgtggcaac acgtacgacg gtcggcaggt
     1201 ttggcggtac gccaccgacg ccgagatcga cgccatggat ctgagcgtac tgccctcgtg
     1261 tctcgcgggc cgcgcgttcg ctgagttcgc ccctgcaccg atcgcagact tcccgacgga
     1321 cgacgacgac gacaacatgc cgaccgacga cgacgactaa tttcgcttat gtggggggag
     1381 ggcacgttgt cctcccccgc ttctttcact ttccgcattc ggagacattg actatgagta
     1441 ttcctgcttc cattcctacc ccgttgccgg agcagatcat cttcggctcc gaggggcgcg
     1501 gggagcgccg ctgtgcgatc gcgtggataa atcaccgctt cgctggcaat gccagtatgg
     1561 ccctcaaagc ccgcaacacg tttgtccaga tcgcggcaga gcgcggttgg ggtttcgctg
     1621 atgccagtgt tccccacaac aacgactcgc agaagccgct aggcgagacc ggtcagcagc
     1681 gtatcgccaa cgcgctccat gagacgttcg agcggcttgg catcccgctg aacggtaagg
     1741 agatcatctg agacgagggt tgactccacc cccctccacg gaggggggaa tagtccaccc
     1801 ttgggagcct cagttgaaca agcaccacgc cccccggcca cgcgaccccg atcgcacgac
     1861 cggaatcacg atcgtcatca tcatcgtcct ctatgtcctc tggcacagga gcctgtgact
     1921 atgcaacaac gtcatcgcct cgaccgaacc ggcgagaagc cgaacctcag cgcccactgg
     1981 cccggaagcg cgggctacct ctactgtggc cactgtcaca agcctatgga gcggcccggg
     2041 gtttcctact gctcgatcgt ctgcgcgaaa gccgagtctg ccctccgtgc aaagggggag
     2101 gcccttcttc ggacctccga ggccatggcg aacaacgaac gcgctgcgag atcggcggga
     2161 gcgcttcgcg aagacgggag cgttcgcacg atctgggacg atgacgctct ggacggaccg
     2221 tcgctgatct gccctccgcc caaggacaag gggaacggat gatccgccgc atggcccttg
     2281 tcgtctacac cgattggcca gaacactcag tcgaagtgtt ccgccttgcg gggcacgcac
     2341 gattcgagga ggcggtgatc gggttcgggg ggattcttct ccccgatcga catcgtcctt
     2401 gcttctgcga ggtcttctat tccgagggcg gagaggaggg cgcgatcgcg ttcgcaattg
     2461 aggattacaa aaacgcccta gaggcgggca agcgcccgtt ctggccggac ccatcacagg
     2521 agaacaagca atgagatgcc cctactgcac aagcgtacaa gtcgaaggag aggcggtagc
     2581 ggtttttacc gctggcaccg agcagacttg cacctgcacc cgctgcgccg gaacgtggac
     2641 ggacgtgtac gcatggacgc acgtggtccg aacgcacatt ccgtcactcg cgccgcgtcg
     2701 cgccgactcg atccagtgtc ccgcgtgcct cagcacgaac atcaccgcat tcgattcgat
     2761 cacccagcgg tacaccgtcg ccgacgatcg tacggacgtg ctgatgctca actacgccgg
     2821 aacgtgcgac gacagcgcga gcgaagtgtg gctggaatgc gacgactgcc atgaggaatg
     2881 gccgatcccg agcaacatag aggtcgatta cacttgacgc cgtgaggcga agtgctagac
     2941 tgacttcgcc tcgctcctcg cattgggaga cttcagaatg atcttcaccg actcgcaggc
     3001 tcttgcgcac ttacgcgaat cccatgtccg cacgatcgag atcgggcgaa cgtggcgaaa
     3061 ccgcaacgtc gcgctcaagc acgaacacga gttcgacatg ctgatccacc acgaggacgg
     3121 ggactactcc ggcgcgtgga tcgtctactc caaactcaat ggggcgtacc gcaagaagcg
     3181 ggagacgccg cagcgccctg taacgtaagg aaccacagat gagttacgga atctacatga
     3241 atacagcaag ggtgcgattg tctcgcggtg actttgacgg cgcgagacac gcttgtgatc
     3301 ttgccattga cgaagcgaac cggattctcg atcgggcgtt ggcaatgtcc aatgaaatcg
     3361 ccttcgagag acgccaagca ccgcgagaaa aggggctaaa cgcgattctc gcagacaagc
     3421 ccgccgacat cccgggtgtc gaatgatcga catcgctgca cgcattgtga ttgaccatcc
     3481 cctgctcgcg ctcgccatcg ctgccttgct gggctacgcc atggcgaccc tgattgacgg
     3541 actcgcgttc cgtcggcagg cgagagcgat gagaaacctt cgtcacaaga ttcgcatgct
     3601 ggaactcagg ctatgagcat ccagcacgaa cctactgaat gggacggcgc gaaagcggtg
     3661 cgagaacgcg tgctgatctt cgccacacgg catcggctca acgggtcgaa gctgctcccg
     3721 acgttccgtg cgatcgcgat cgagtacgag gcgatgcgtc gcgcgcacca catcgaccag
     3781 agcatcatcc gggatctgtt tcttgaacga catcgtgttg agcggctcct gcatcggcat
     3841 ggacccgact cggcaattgc catgctccgt gctgttatgc cgcacgccac ggagtctcaa
     3901 atacggcggc tattggagca ttcgagatga acattgtgga gaccatggct cagcttcagg
     3961 gggagatcgc tgaggcgcag gcgaaggtaa acgcggcggt gaacgacggg ctgaaagcgt
     4021 gcgagcggat catcgagatc cgcgacgagc ttctcctcgt ccagcgccac ggacgaagga
     4081 gcatcagggt cgtcaagagg cgcggaccgt acaagccgaa ggcgacgccg gtcgatgcca
     4141 cgagcaacgg gcatggggga acgaggcggg cgaagactga tcgcacggtg accgccattg
     4201 atctgctgac gttcttgagc gacggcgaga agacgaccaa ggcggtgaag gcgtattacg
     4261 gaccgaagcg atggcagacc gcacagtcac agatggggaa ccagcggcag ttccggcaca
     4321 cgtcgcgatc caacgggatc tgggccatca cgaagaaggg cgcggagtac cttgaggaga
     4381 agagcgtcaa tgcctgatgc gaacaactca caccgatcgc gtgtcctcct cgaaacggat
     4441 ttctcgttcc tcgaaagaga agacgagaag accaaggcga aggagaagac ggtccacgtc
     4501 ccggaattct ggcgggtggg tggacgattc cattcggcga agtatcgctt tctccaacat
     4561 tgccgacgca tggagtcgtt cgccaacgcc atcgacaaag aaggcatacc ggcggggatg
     4621 gtgaaccgtc tcgaccgcgc ggtcacgctc gtgcaggagg ccgcactcaa cagcaccggc
     4681 aagcatccgg ggaagaagaa gtagaattcc cccctgtgcg cgggggcggt gggtttcccc
     4741 tcaccgcccc tctttcgtcc ttcctttctc ctcaccaccc attcggggga acccggatgg
     4801 gtgggtttct cccaacaaaa aaacggtgcc cctccggaga agggcaccgc cgcattggga
     4861 gaccatcgac gacgcccctg agcccgaggg ctcgaaggaa acaccatcca tgaccgagac
     4921 tcagtctacc ccgtcccgag cgattgtcta cgcccgattc tcccctcggc ggaatgagga
     4981 cgaaactgag agcatcgaga cgcaggtgga ggcgtgcgag gacctctgcc gcaagcaggg
     5041 ctacgccgtc gaggcggtct tcagcgaccg ggcggcgagc gggaaggatg aggaccgccc
     5101 cgggctctgg gacgccgtac gctcgctcaa gaagggctac gtactcgtcg tctacaagtc
     5161 cgaccggctg gcccgcaatc tctacctgtc cgaatggctc aggcgggaga tccgggccaa
     5221 gggggcgaag ctggaagtcg tgagcggcat gtccgagggc gactctccgg aggccaccct
     5281 gatccgtcag gtgctcgatg ccttcagcga gtacgagagg aagctgacct cagcccgaac
     5341 cagcttcgcc atgctcaggc accagaggac gggacgacgc atgagccgcc accctccgta
     5401 cggatgggac ttcgatccga gcgatccgca gcggtggatc cgcaacgaag aggaggggaa
     5461 ggcggtcgag aagatcctcg actggcgacg caagggcgcg gcgtggcggc ggattgcgac
     5521 cctgctcgga gaggagggcc acaagtcgag aggatcggag tggcagggga agaccgtgaa
     5581 gaagatattc aaccgggaaa cgacctagtc gtagccgatg gagatatcaa ccccggcggc
     5641 ttcttccgta ccaccatgac tcgcggtcgt cgcggcgatc acgagaccat tcttgaacaa
     5701 caggccagag gacgtgaagt gcgcctcgtt ggcgagcccg ctcccgacga actgctgcca
     5761 cgccgggagc gttgtcccga gcgtcacgtc actcgtcgcg gcggcatcga agaactgcac
     5821 gaacgtggtt ccgccggaaa cattcgtgat ggagtacgcg cccatcttca ccggcgttgc
     5881 cgacacgagc gagagggcgg tgacatcggc ttcggtgtac cagctttgca tcagcggacc
     5941 cctatcttct gcaccccggc gcggagcgcg gcagcggttc cgaatccgag cgcggcgagg
     6001 atctggtcga gatgcaggcc gatccacgat tcaggcgcgg agtttcccca catcaggagc
     6061 gcgatcccgg cgagagcaag gatgtacgtc ttcgagcctg agaacgcttt gaggaggtca
     6121 ttcatggtgt gttcctgtcc ttgatctgct ttcccgcgaa gatcagcgtc acgagcgaga
     6181 ggacgcccgc cgtgatctgc ccgaacggag gcggcaggaa aggcgcaacg gatccggcgg
     6241 tgtccataat gacctcaccg ggctcgacct cgcgaccgtc gcgaaggtcc gcgatctgct
     6301 ccccctgcgc cgtgatctcc gcctcgagcg cttccttttc ggcgatgagc ttggcgagct
     6361 tggtttgtga ttcggtgaac tcttccggcg tcgtgtctgg agcctcgagc tttgcgagca
     6421 gggcttcgat ctgtctctgc tggtcgatga tcctcgactg ctgcgagtcc acgatctgct
     6481 cgagacgatt cgcttcagcg agatcagcgg aagagggcgg gagcacacac ccgcccacga
     6541 gcaccccgag gaggcacacg agtcccagca cgatggcgat gacccacaca agatgaagaa
     6601 cacggcttcg gtcggcgtcg atattgacca tggttttacc tcggttgctc gatcgggatg
     6661 tcgtcaacga ccggcatctt cagctctgga ttcaattcat ggaatttccg ccaagctgcg
     6721 cgctcgtgcc accggttcca tatctccgtc ttcggtcgct gtgaactttc gagttgcact
     6781 aagttgcgac ggatgtcagc aagctccgaa gacggagcga ggagcgaaag cgcagtcttc
     6841 acgctttcca ttgacgcgag gagctgctgc gctgtggact gattcgcccg tatggttgtc
     6901 tcgatcgtcg ccttccatgt cgcaccgaaa tagacgaccg ccatcgtcga tccgaggatt
     6961 gacgcggcaa gagcaagctg gaccttgctg tttcgggtga tcgttgtttc ggatgctggc
     7021 tcagacatta cacccccggg ggcaaaacgg tatcgatcgt gacacctttt ctgaccaccc
     7081 gcagggggat atcggcgtac ttcgcccaaa gcttccgggt gcgtcggaac ccggcggtct
     7141 cgacaccctt cacgtcaaca gcatagatcg tcccgttgcg ttcgacaaca aggaagtcgg
     7201 cggcgtaccg gtcgtcctcg ccgagatcga agcggggctg acggagccac cagacgacat
     7261 cggggtcgag atcgagctgc tccgcgtgcc tccgctcgtc aagagagtcg tatacacgtc
     7321 cttggtacgt gcggcgtgcg cggccctccg ccgtctggtc cacgccgtac ttgcttctgc
     7381 cgaatttgcg catccttgcg tctccgctgt tttcggtgaa tgttttgcgg gctgtcgccc
     7441 gcagcgcgtt gcacgctgaa atggttttcc atcactacgt ggtggggtgg ggagttttag
     7501 gagccgctcg ctgcgctcgc tccatctgac ggcgaccatg atcaacatgc ttctgtcttc
     7561 ccaagcgttt cattccttga accccgcgac atcgccacgc gacacatggg gcgtgattcg
     7621 accgaaccgg cgttcggggt gacgcgcttg gatgcactcc cggcacgcca gcgtctcctc
     7681 gccatcgact acgtgcagga acaggcactc atccgcagga gcggggtggc cgcaaaagca
     7741 gcacgcattg cgggcctcgc cacgcagggc cgggttgaac ggtgttgttt ttcgcatcgt
     7801 tccgttgctc ttcgatcagc ttcttcagtt gcttttcgtt cgccatagtg catttccttc
     7861 gatccggtga gtgcagagcc cctatacaaa ggagccctgc atcgcgttca tttcctttcg
     7921 agagccgtat gagacgcttc gccctgctcg ccactttaca aacggtcgat tgaggggcat
     7981 tgatcgccgc gacgtggtga gccgccccgg tacgacttcc aatcgatccg ggactcgccc
     8041 ccgcgcacaa ccaagggcgt aggcggggac gggaacaatc cggcgcgcca ctctggacgt
     8101 atgaggccgg ttctctgctt ggtttggcgc gcacgctagc aatcccgacc catggcgcgc
     8161 tcgctgaggc aggatatcca ccggttatcc actggatacc ctgatcgcgt gcgcacgctt
     8221 caccggatcc gtggcgcatt cggcaaggtg catgaacaga ccctcgcgga tctggctcgt
     8281 gagccggtcc gtgaggcatc gcatctcgtg gggcgtgcgc cgcaggagca gctcacgatg
     8341 gtcgatgctg gcggtataca tgatgtgcga gaccatggcc tgatagctct ggacgggccg
     8401 gtgcagctcc gcatgcgggg tgagcatcgg gaccggctgc gagagcagga accgccgtgg
     8461 gccgtccttc gcgctcgact ggtagtccgg gtgccagaaa tccgacgtgg cgagcgggac
     8521 gctcacttcg tcctcacact ccgtcagctt cagcagcatg tcgatgttgt cgaggatggg
     8581 ctccatgtcc cgggcgtacg cacgaagctc tctcagctcc ctgagctgcg tggagtccat
     8641 gcgggacatg gctttccgct tcacccaacc gagcgcctgt gagatcacgc cagctcgccc
     8701 ttctggtgga cgttgaacat cagcatccgc agcatccggc tgtcctctgg gtagaggtct
     8761 tgcaggaagg ccgggatccc gggctgagcg agggtggctg cacgcaatgc ggtcagctcc
     8821 tcctcgctca gcccgccgag cagctcgtca acgagcaccc gttcccgttc gaccttcgcg
     8881 cactccagac cgaaccgact gcgacgttcg gcggatgggt ccgccttggg gaagaaggtc
     8941 tctctggtgt ccatcagaac ggaatgtcct cttcggcgac cggggcgtgc ttctgcgacc
     9001 cgatggcggg ctgcgcgttc agcgcacccg taagcgggtc ctgacggaac ggcggcgttg
     9061 gcggttgcgt tgtgaccggc ggcgcaaccg gctgcgtcgc aaccggtgca gcgggggcga
     9121 ggttgccgcc ccaatcgtga cggatccgga tgcacgagac ggtcttcttg cccatctgcg
     9181 tgcgcgtcgg gtagacccgg atcttcttgc cgggccaatg ctgctcgatg tcgtcaccgc
     9241 cacactgaag gtcttccgcg atcgtgttcg cgttcgtcca attcaggccg agcttcttct
     9301 cggtctcgtg gaagtcgatc acgatctgct tctgcccgtc gtcaaactgg tgctccccga
     9361 tcacggtcct gatcgtgagt gcgtagtcct gtccgttcag gtcagcggcc ttgagccatt
     9421 cgccggatgt gtacgtgtcc ccaatgttca cttggaatct cctgtagaac ctttgcatcg
     9481 acctttgaga agacccttcg ggatctccgc aatcttgagc ttctcgtcca ccgtgacctc
     9541 gaagttctcg tcccacacgt ccggtgacag aatcgtgcga gcagcgccgg gcttgatggc
     9601 attcgcacca agacacgaca acaaagcctc cacatcccca ccgctggcgc tgaagacagc
     9661 agaggcagct ttgcgactgc tgaggcatcg ggttttcttg ggtcgcgtca ccgtgacgat
     9721 cttgtcgccc atctcgagcg acacgaccct gtgttcgttc atccattcga tcatggcggc
     9781 atcgaacgct gccgcctcct ctttgacgag cgcgacggcc cgcagcacag cggaccgccg
     9841 ctccagaatc tcatccggtg tcatctccgg accgaacagg tgtgtgcgcc ggagcaccga
     9901 caggttcacg atcggtaggt tcacgcttgg cttgtcgttg ggcatcgatc attccatgga
     9961 tggcagggaa ggctttgggg gcggtggtca agatctcatc ggcggtccgt gcgcaccagt
    10021 ccagatcgat tttcgcgatc tcggcaagca cccggtcccg gtccgcctcg gtctcgacgt
    10081 tcaacagcat ccgccgcgtg accttcgcac ggaatgtgcc ttgagcgttg cgacccgcgt
    10141 acccgggctt caggacctcg aggagcttct tgcggacggc aagggccacc ggctccttga
    10201 ggagcgccac cttcctcacc atcgcccccg cgtcaccgag ccgctctgac cgctggcggc
    10261 gtgcctcagc ctcctctgga tccgtcagga tctccacggc ggaagcgatc cgctgaagac
    10321 acccgatgcc gacgaggcac gacgctgcgc tcagctcgtc cctcgcgagc ccctgtggct
    10381 tctccggcgt gccccatttc tggcgcgaca ccttatccca aatgaacgcc tcagattcag
    10441 ccatggttct gcttccgtgc ttggatgtat ctggtgatgc tttcttccgt gaagcgccaa
    10501 cagccaccga cccttatggc cggtaaattg tctgtacgta tgaggttata gacggtcttc
    10561 tggtggacac cgagccacaa agcgacctgt gccgtcgaga gtagattgcc ctcatccata
    10621 ggggcagcat cttaccagat tatgccagaa tacccatagg cgaccagcga acgaccagcg
    10681 aacgaccagc gaacgtccag cgcgtggatg gccgtggatg gccgtggatg gccgtggatg
    10741 gcctactggt tcgggtcgcc gatgccagcc ttccgccgct tgatgagatc gtccagcgct
    10801 cgctcccgct cacgcatctc gggcgagcct gccttgagct ggtttatccg gtgcgtcagc
    10861 tccagacgcc tctgctcacg gtcgtaggcg gcgatagagg ccgggtcgcc ctcgagcatg
    10921 agccggtcgt agtcgaggat ccgtgagcgg actccggcgt cgaggacgaa ctgaaggtcc
    10981 tcctccgcct tcttcgccat ggcgtcactc ttctcgtcgc cgcccttccc tgcgatcgcc
    11041 tcgcggagat actgcgtcgt cgcgccgagg ttcatcaggg cgacgagcgt gtcggtggag
    11101 tcgtccgccc gctccatttc cttgatgagc cggttgaggt aggccgactt cagacggccc
    11161 ttggcctgcg cgaagatatc cctgcggccc acccgcccgt tgagcttcag cgcgagatcg
    11221 acccggcgaa gatcatcgat caccgccttc tcctgctcct ccttggagat gccgcgcagc
    11281 tccttcgttc gcttccacca gcccggctcc gcccacgccc gcatcaggtc ggtgtattgc
    11341 tcgcgggcct gccatggcgt catgccctga cgcttcggga acgtaaagaa ggcggtgttg
    11401 ccggtgaggc tgaacggagc gaagcgactg aggatatgca agccccgctg gcccatgttc
    11461 tcgagaccac gcgtccgcag gcggaacccc gtgccggtcc acgccatcgg gaactccgac
    11521 ccggcggagt gcccgtatgc ctgctcgccg atgatttgga gaagtcgcgc ggctttgttc
    11581 cccgcgctct cgatgaggcc ctttgggaag tcgtcgaagt agcggaagat ttccttcacc
    11641 tgcttgcccg ggctcatata gaaccgctcc agcccgatcg gaatgcccca gaaccggtgg
    11701 aaccatcgat agaacggcgt catgtcgata tcgaactcgt gccccggctc attgagccac
    11761 gggaacggct gcatgtccgg atcttcgtcg cgcgcccaga ggtagatcgc gtgctgcgct
    11821 gccgccatca tgccgacgag cacaccgaac atgttcgacc agatgcgcag cgtcgccctg
    11881 aaggcgtaga agttgttcac ctcctgcggc cccggaccac cacgattcgc ctcggtggac
    11941 tttcctgcgg acagccgcga ggaggtcttc cgctcgatac gaatcggaac acgaacgcga
    12001 gtcagcgcac ggatgatgtc ctgcccttcc tgaagcccaa catccctgag cttcttgcca
    12061 acaacgggga tatccatgag accaaaatcg agcgggatga gctgtggaag gttccgccga
    12121 acgaaccgtg atgtctcgag cggaagcgat gcggcttgac gaatctgcgc gatcgtccag
    12181 tccggcgcga acactacctg tgcgatccac tggtgctggc gaatggtgat cggctccgcc
    12241 catgcgaacc cactgtcgcc accaaaccgg gggaggttca gctcacgctg gccaccgagc
    12301 gcattgcgta cgacgtgcgc cgccgtcgcc cgtgcgtcct cgatcgacat gccgcgacgc
    12361 gccaactggt ccttcttctc gtagacaaca acaccatcct tggtggtccg gcccgtccac
    12421 tggttgacga gcgagtggta cgagaacagc acgaggcccg cgtgtacgtc ctcccagaga
    12481 tggcggttgt acgcctccag catcctttcg ttgagcttgg cgagtcgccc aacgagcggg
    12541 atgcctttcg ttccggcgct gacgctgcgc gacagcttct ggatgtattg ccgcgcgtgg
    12601 tcggtcgatg cgggcatgtc gagcgggagg cccgccgacg cggaatcctc gagaccgtac
    12661 agctccgcaa gccggtgccc cgccgtcgct gtgaacatgg ggccgaagga cttgatgccc
    12721 ccgcgctccc ccggaaactg gccccgggcg ataccaagcg agtgttgctc cgtcggcata
    12781 atgagcatgc cgcgcagcac ctgcccacgc tctggtcgtg cgttgagggc cgtcgatgac
    12841 tcgtacagtg tggcgtggtg gaagagcgag taggagacgt tgagggcctt cgcgtactgg
    12901 ttgatcgcgt tgatgacccg cagcgtgcct tcggccatgg gctcctgaag gaccgcctga
    12961 agaggccgca cgatttccgg atggacgtag gtgttcccct cgaagagtcg gaagacaccg
    13021 tccttgccga cggtgccaaa gaaccgattg agcgcctgaa tatccacctt gacgtactgg
    13081 tgctcaccgc tgccgacgta atccggatca gagtgcgaat ggacaatgag cttccgcccg
    13141 tcggcggtac ggaggctgcg gagatcatgg acgaacttga gcgtcgccgc ggcgcgatag
    13201 ttacgctcgt accaccgctc catcgtgaac gcgagatcgt ttgtgagtgg acgcagcggg
    13261 aagtccggat catcaaccgc gtcggcgagg gtcggatact tccgcttctt ggagtgcttg
    13321 gtcttcgtga tccacttcgc ggcgaagttc gaggcgtccg cgtccggaac ctgatactca
    13381 tggccgacgt agttcgggag ccatgaaaga aactcgctgc ccttcccgga aatctccgcg
    13441 atcttgttca cgtccatgcg ggcttcttcg tgcatgaagc gagcttcgcg gaggaggcga
    13501 tcgatcagcg gccagttcgg attggcctga agccgctcca tgaccttctc gtaggagtcc
    13561 tgattgccag cgggggcgat gtcgtagtag ccgcccggca ccgtaacgcc gaccacactc
    13621 ggctggcccg ccttcggctg gagcggaatg ccgctcgcgc ccttgacctt caggaagttg
    13681 ccgtcggcat cgacgaacac gttccgcgtg ccctcgatcg cggcgtgcat gtccatgcga
    13741 acagtctcgg ggaacatccg ccgcagcttc cgcgtgaacg tgtacgcccg cgccgtccgt
    13801 ccagcatcga gattcttcgt cgccctcgcg tcggcgatgg cctgctgccg cagggcgggc
    13861 tccagcgcga gcggcccatg aacgagcggc ggcggaatgt gggtgtgctg ctcactgcgg
    13921 ctcacgggcg gcgtggggcc gaccgagatg ccgttgtgga gcatgttgaa gatcgcgcga
    13981 gcgaacgcat tcgcggcgat ctgctccttg acgttggcga tgaactccgg ggccttctct
    14041 tccgcagcgg cggggtcgat ggcgtacagc gcgagcgtat cggcgaccag ctcctcgttc
    14101 tggaggcggt agtggatgtg cttgttgttc gattgccacg tcattcccaa cgggagcggc
    14161 ctgaagttct ccgacgccgc cgtcagctca cgcctgagcg ccttctcgcc gattccgatc
    14221 cccgggacgc ggtgcttgat ggtcttgtga aaacgtcgct tcccaccgag cagcacaaag
    14281 tcgagcccgt ggcccatacc ctcgtgcgtc agcgtcccaa ggacacgagg atcacgcaca
    14341 tgaatgaaag actgacccgg catcgatcgc atggcggcga ggcccatgat cttcgcgccg
    14401 aggatcttcg gcatcaccgc accgcgaagc tcgttgctcc cgacctcacc gagcaggagc
    14461 cgtgttgcct cccacgcatc cacaaggaag ccttcccgtg ggtggatgta ggacggcagg
    14521 tggtcatcga gcggattcat ctcaccgtac ggaacgtgcg gccagaacgc cttgagtgac
    14581 ggaccctgcg gctgaatgtt cgctcgcccg gggagcttgt ccaaacctcc gccaccaccg
    14641 aacgcggaat ccacatcgct gtcgatacct tcgatgcggg cggcgatgtc tcggtcgccc
    14701 ggcgtgttct cgggccggaa gaagatgccg ctcgcgcggt acgcctcgaa gaccttcttc
    14761 gcctgctcga gattgacgcg catgttcatg cggccaccgc cgctgacgaa ctggttcggg
    14821 gcggcggcgg cgagcgcctt ctcgtgcagg taatacttgc cgccgcgccg ctttgcctgc
    14881 acggcattga accgcacagt ttgcccgccg ccatcgaggc tgacgatcat gtcgccctcc
    14941 tcgtccgcaa agaccggctt gcgtgcttcc atcatcttga agatattctc gacgctcgag
    15001 aacttgatcg ggcgtgcgtt caggaattcg ttcggggtga atgacggcgg catcaggatg
    15061 ccctgacgca ccttgccttc actcgtcgtg aacatgatga tctggccccg gttattcacc
    15121 tgctccgccg cagcgagcag gttcccggtg gccatgaacc ggatctcccg ggcgtcggtc
    15181 gcggcccact ggaacacatc gaggatgcgc tcgcgtgtga acttctgctt cgcggcaacc
    15241 gagtagggcc ggatcgcgcc ggggatgtcg ctcttttgga gcgtggtcct cggctcacca
    15301 gcgagcggca cgatgcgaga cacaaagatg ccctgctctc gccacccctc cggcgcggcg
    15361 acgatcacct tccagtggct tccggcgacg ggattgatcg ctcccttcgt tcgttcgtag
    15421 cccaccacga cgacgagctg cggcctcgcg atgtcgatcg ggttcttgac atcgttgacg
    15481 agcgcggcaa cggtgccggg cgggagcgtc tccatcaggt cgctgaggga tcgaagctgc
    15541 gcctcggtca cggcggtgat ctgccgcttc acatcc
//
LOCUS       DASRZF010000045         2456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2097842, whole genome shotgun sequence.
ACCESSION   DASRZF010000045 DASRZF010000000
VERSION     DASRZF010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2097842"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            254..838
                     /locus_tag="VFG22_02075"
     CDS             254..838
                     /locus_tag="VFG22_02075"
                     /inference="COORDINATES: protein motif:HMM:NF021455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET6415055.1"
                     /translation="MVGSVYCALVHHPVVDRQGEEVTTSVTNIDVHDIARSARTYGLR
                     GYFVVSPIEAQHLVVQRIIDHWCSGAGKERFPERGEAIALVRLSASIDEAIASIEAEE
                     GNPPRLLATSARPGTGRSSYAEEAARLRTEDRPTLLLFGTGHGLSDRVLQRADVFLEP
                     IDGPGEFNHLSVRAAVAITLDRLFTGKSLANDPI"
     gene            892..1257
                     /gene="rplS"
                     /locus_tag="VFG22_02080"
     CDS             892..1257
                     /gene="rplS"
                     /locus_tag="VFG22_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004415194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HET6415056.1"
                     /translation="MSGNVPEIQAIEKSQMRESTPFRVGDTINVHFRIREGEKERVQV
                     FKGVVIRKSGGGAGATFTVRKVSGGVGVERIFPIHSPRIEKIEVVARGHVRRSRLFYL
                     RDLRGKKARLRERQRQTND"
     gene            complement(1262..1768)
                     /locus_tag="VFG22_02085"
     CDS             complement(1262..1768)
                     /locus_tag="VFG22_02085"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HET6415057.1"
                     /translation="MNGEPLPWISGDLEIDKSVMDEIEQHALECYPSESCGFVFGPAD
                     RPAVLDALQREENEADKYHALDPVTFPRTSNTYFKINELRAARTFEQGEEKGRPVKVI
                     YHSHCDAGAYFSDEDAATFANGGQLMWPCAYIVVSVMDSKVAERRLWVHEPGTNRFRE
                     STLTIREA"
     gene            complement(1758..>2456)
                     /locus_tag="VFG22_02090"
     CDS             complement(1758..>2456)
                     /locus_tag="VFG22_02090"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HET6415058.1"
                     /translation="GNTGVAYSLFGAAMGYRIQLVMPLNVSQARKDIARAFGTEIIFS
                     DPMEGSDGAIRMVRDLVAGDPDRYFYPDQYSNPSNPRAHYLGTGREILEQVGDEITHF
                     VAGLGTSGTCMGTTRRLREHSRKIECVAVEPAEALHGLEGLKHMASSLVPEIFDPKLP
                     DEILSVSTDAGWDMSDRLAAQEGLHVGHSTGANVAGALEVAKREEASCVVAIACDRGD
                     RYFAPMKWEKHYEW"
BASE COUNT          443 a          769 c          763 g          481 t
ORIGIN      
        1 cactggcact gggcgctggc actgggcgct ggcactcacg ctggcgctga cgctgccgta
       61 gggaggagga cggctcggga tgggcagcgc agcgattggg tcgagcctag ccgtgggtcg
      121 tcttatggaa ttgggggcga gtgggtccca atcgcgagca cgtgagtcgc gagccgtcct
      181 ccggtcgcac agtcgcgcaa ccacagttgc tggcactgcc gctgctttgg ccccaatcgt
      241 gtggtaagtc gccatggtgg gttcggtcta ctgcgcgctc gtgcatcacc ccgtcgtcga
      301 ccggcagggc gaggaggtca ccacctcggt gaccaatatc gacgtgcatg acatcgctcg
      361 gagcgcgcgg acctacggat tgcgcgggta cttcgtggtg agcccaatcg aggcacagca
      421 tttggtggtc cagcggatca tcgatcactg gtgctcggga gccgggaaag agcgctttcc
      481 ggagcgggga gaggcgatcg ctctggtccg gctctccgct tcgatcgacg aggcgatcgc
      541 ctcaatcgag gccgaagaag gcaatccgcc gcggctgctc gcaacctcgg cgaggcctgg
      601 aacgggtcgc agcagctacg ccgaggaagc ggctcgcctc aggacggagg acaggcccac
      661 actgctcctg ttcggcaccg gacacggcct cagcgaccgt gttttgcagc gtgccgatgt
      721 ctttttggag ccgatcgatg gaccgggcga gttcaaccat ctctcggttc gggctgccgt
      781 cgccatcacc ctggatcggc ttttcacagg gaaatcattg gcgaacgacc cgatttgacc
      841 cccttttggc cccgtgctaa ccccgggctc cgtttcggaa gaggcttagt gatgagtgga
      901 aacgttcctg aaatccaagc gattgagaag tcgcaaatgc gtgaatcgac cccgttccgc
      961 gtgggagaca cgatcaacgt gcacttccgc attcgcgagg gtgagaagga gcgtgtccag
     1021 gtcttcaagg gcgtggtgat ccgcaagtcg ggtggaggcg cgggcgcgac cttcacggtc
     1081 cgcaaggtct cgggcggcgt tggggttgag cgaatctttc ccattcactc gcctcgaatc
     1141 gaaaagatcg aagtcgtcgc acgcggccac gtccgccgat cgcgcctctt ctacctccgt
     1201 gatctgcgcg gcaagaaagc tcgtctccga gagcgccagc gccagaccaa cgactgacgt
     1261 ttcaggcctc tcgaatcgta agcgtcgact cgcggaaccg gtttgttcca ggttcgtgta
     1321 cccataggcg tcgctcggcg accttgctgt ccatgacgct caccacgatg taggcgcagg
     1381 gccacatcag ctggccgccg ttggcaaagg tcgcagcgtc ctcgtccgag aagtatgcgc
     1441 ccgcgtcgca gtgcgaatgg tagatgacct tcacggggcg acccttctcc tctccctgct
     1501 cgaaggttcg cgccgcgcgg agctcgttga tcttgaaata cgtgttcgag gtacgcggga
     1561 aggtaaccgg gtcgagcgcg tggtacttgt ccgcttcgtt ttcctcgcgc tgcaacgcat
     1621 cgagaaccgc cgggcggtcg gccgggccaa agacaaaccc gcaactctcg ctcggataac
     1681 actcgagcgc atgttgctcg atctcgtcca tgaccgactt gtcgatctcg aggtctcccg
     1741 agatccacgg caagggctca ccattcatag tgtttctccc atttcatcgg agcaaagtac
     1801 cggtcgcctc ggtcgcaggc gatcgcgacg acacaactcg cttcttcgcg cttggcgacc
     1861 tcgagcgcac ccgccacatt cgcgcccgtc gagtggccga cgtgcaatcc ctcctgggct
     1921 gccaatcgat cggacatgtc ccagcccgcg tccgtgctca cggataggat ctcgtctgga
     1981 agcttcgggt cgaaaatctc cggaacgagc gagctcgcca tgtgcttgag tccttcgaga
     2041 ccgtgtaggg cctccgcggg ctctaccgcc acacactcga tcttacgcga gtgctcgcgc
     2101 aaacgacgcg tcgtacccat gcaggtaccg ctcgtgccga gccccgccac gaagtgcgtg
     2161 atttcgtcgc cgacctgctc gagaatctcg cgtccggtgc cgaggtagtg agccctaggg
     2221 ttcgacggat tggaatattg atccggataa aaataccggt ccgggtcgcc ggcaaccaga
     2281 tctcgcacca ttcggatggc gccatccgag ccttccatcg gatcactgaa gatgatttcg
     2341 gtaccaaacg ctcgtgcgat gtccttgcga gcctgcgaga cgttcaacgg catcacgagt
     2401 tggattcgat agcccatcgc cgcgccaaac aggctgtacg cgacgcctgt gtttcc
//
LOCUS       DASRZF010000046         2426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3127261, whole genome shotgun sequence.
ACCESSION   DASRZF010000046 DASRZF010000000
VERSION     DASRZF010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3127261"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VFG22_02095"
     CDS             complement(<1..242)
                     /locus_tag="VFG22_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415059.1"
                     /translation="MLNRESDGLRDGEESVPGDAELVASLQLGHPEARAAFFDAYGEF
                     VERLLIRVLGRDSEIEDLLHDVFVQAFANIGQLRDPT"
     gene            435..941
                     /locus_tag="VFG22_02100"
     CDS             435..941
                     /locus_tag="VFG22_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415060.1"
                     /translation="MNSRLTWSLLGAAFLLAQGCGEPSAGSSELANRQASGSGGGSGM
                     ACESDLDCPEGEECELEHGDSFCKPHGGDQNGEDGGMVACESDLDCAEGEECELEHGG
                     SFCEPQDDDNQNCDGSSDDDGHEGADCSDDDSDSDSDDDGNDDSDDSDSDSDSDDDGQ
                     GGTDGGQA"
     gene            complement(1070..1255)
                     /locus_tag="VFG22_02105"
     CDS             complement(1070..1255)
                     /locus_tag="VFG22_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415061.1"
                     /translation="MREANPNTRTIFLMLALIAAVEIFRPAPALTQRTTEQSVRDVVY
                     ELRGIKQELAQIERKMK"
     gene            complement(1293..1760)
                     /locus_tag="VFG22_02110"
     CDS             complement(1293..1760)
                     /locus_tag="VFG22_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415062.1"
                     /translation="MTNEERQGEMGRHLLQLGVLLFFIGLLTGFALPLAANPRMALSS
                     HLEGVMNGLFLLALGAIWHRLRLGLRALRIAFWLAAYGTFVNWGTTLLAAIWGAGEKM
                     MPLGAAGHTGSSAQETLIAFGLISLSIAMLVVCPMVLWGLRAGADAGGRTRLS"
     gene            1963..>2426
                     /locus_tag="VFG22_02115"
     CDS             1963..>2426
                     /locus_tag="VFG22_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415063.1"
                     /translation="MPRFIRCASILFACLALSAFGSTGCGDSSESSAGTGGMGAAGTG
                     GTGGSSGFGGQGGDGAAGGAGGTGGIIAPKEQEVLLVGGFMSEIYTELSLHLEDELNA
                     ALRNKARTLNVHIDLPLNQSIDIAIGDAIANALPRISLPLEPGGFISFHTQEA"
BASE COUNT          504 a          822 c          699 g          401 t
ORIGIN      
        1 gtcggatcac gcagctgacc gatgttggca aacgcctgca cgaacacgtc gtggagaagg
       61 tcctcgatct cggaatcgcg cccgagcacg cgaatcagca agcgttccac gaattcgccg
      121 tacgcatcga agaacgccgc gcgcgcttca gggtgaccca attgcagtga cgccacgagc
      181 tctgcatcgc caggaacaga ctcctctccg tcgcgcagcc catccgattc tcgattgagc
      241 atgctggctg ttttggacac ggccatcgag ccgtccatta tgagactagt gtccacaggc
      301 ggctatcccg gctcatttgt atgaagttgt aaggatccga accgcttttc acccgactgg
      361 tcacttgagg ggagcgacgc ccggctgggg cctcgcgcag caatccccaa gcccgttcaa
      421 acaaggagca cgcgatgaat tcaagattaa cctggtcact tctcggagcc gcatttctac
      481 tcgctcaagg ctgcggtgag ccgagcgccg gctcatccga gcttgccaac cgccaggcct
      541 ccggctcagg cggaggatcc ggcatggcct gcgagtctga cctcgactgc cccgaaggcg
      601 aggaatgcga gctcgagcac ggcgactcct tctgcaagcc gcatggcggt gaccagaacg
      661 gagaagacgg cggcatggtg gcctgtgagt ctgacctcga ttgcgcggag ggcgaagagt
      721 gcgagctcga gcacggcggt tccttctgtg agcctcagga cgacgataac cagaactgcg
      781 acggcagcag cgacgacgat ggacacgaag gcgccgactg tagcgacgac gacagcgaca
      841 gcgacagcga cgacgacggc aacgacgaca gcgacgacag cgacagcgac agcgacagcg
      901 acgacgacgg acaaggcgga accgacggcg gccaggccta aggcatcgct cccgagcagc
      961 ccgtcacgct gactgagaat gccgctcgac ttggcgaacc tgtacgggtc gccgagtcga
     1021 agcgcgttcc gtcgtgtgtg cggctctccc gctcaacggg tgccacgcct cacttcatct
     1081 tccgctcgat ctgcgcgagc tcctgcttga tgccgcgcag ctcgtacacc acgtcgcgca
     1141 cagactgctc cgtcgtccgt tgagtgagcg ccggcgccgg tcggaagatc tccaccgccg
     1201 cgatgagcgc cagcatcaaa aaaatggtcc gtgtattcgg attcgcttca cgcatcggtc
     1261 ctccaagctc gtttcgagtt tctcgctccg cctcagctta gccgagtcct cccacccgca
     1321 tcagcgcccg cgcgcaaccc ccacaggacc atcgggcaga ccaccagcat cgcaatcgag
     1381 agcgagatga gcccaaacgc gatgagcgtc tcctgcgccg agctcccggt atgaccagcc
     1441 gccccaagcg gcatcatctt ctccccagcg ccccaaatcg ccgcaagcaa agtcgtcccc
     1501 caattcacga aggtcccgta cgccgcgagc cagaacgcga tgcgcaaggc gcgaagaccg
     1561 agtcgcaacc ggtgccaaat cgcgccgagc gccagaagga agagcccatt catcacgccc
     1621 tcgaggtgac tcgaaagcgc catccgcgga ttcgccgcca gcggaagcgc aaacccagtc
     1681 agcagcccga tgaaaaacag cagcacacca agctgcaata gatgccgccc catctcgcct
     1741 tgtcgttctt cattcgtcat gaccgttccc ttctcgtcga cccagccgct cgcaagcaaa
     1801 cggacaaaac caaagcgtgc cggtcatccg aggttgtacg gcagctcttc cagaccgatc
     1861 aagcgccgag tgccgacttg tgtcctgacg cacacccgct cggccccggt tgacccatcc
     1921 gtgccaacta tgagaccttg gcccttcgtt ggttcgcttc acatgcctcg cttcatccga
     1981 tgcgcatcca ttctcttcgc ttgcctagcc ttgtcggctt ttggttcgac gggctgcggt
     2041 gattcctccg agagctccgc ggggacaggc ggcatgggcg cggcgggaac cggcggaaca
     2101 ggtggtagtt caggtttcgg gggtcaaggc ggtgacggcg cagccggggg cgcaggaggc
     2161 accggcggca tcatcgcacc caaagagcaa gaagtgctcc tcgtcggcgg cttcatgtcg
     2221 gaaatctata cggagctgag cctccatctc gaagacgagc tcaacgccgc gctccgaaac
     2281 aaagcccgca cgctcaacgt gcacatcgac ttgccactca accagagcat cgacatcgcc
     2341 atcggtgatg ccatcgcaaa cgccctccca cggatttcgc tcccgctcga gccgggcggc
     2401 ttcatttcgt tccacacaca ggaggc
//
LOCUS       DASRZF010000047         5880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152 k141_33941,
            whole genome shotgun sequence.
ACCESSION   DASRZF010000047 DASRZF010000000
VERSION     DASRZF010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 5880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5880
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33941"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..474
                     /locus_tag="VFG22_02120"
     CDS             190..474
                     /locus_tag="VFG22_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415064.1"
                     /translation="MDFANVLRVPGLGLGVLTQLHRALGALEGTAPLDCGNAGEAVLG
                     KGDIVILKKIEAPDRREVQALARKVRSQARKPGVKRSDIKKAVRASRRRG"
     gene            complement(742..2496)
                     /locus_tag="VFG22_02125"
     CDS             complement(742..2496)
                     /locus_tag="VFG22_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415065.1"
                     /translation="MPQLRISTVASVVLLASLVSACGSDSSSEATAPKGCDYPSTFAA
                     IQETIFEAKGCSADVCHGEQMEGDLNLRTDASYEALVRTPSVIDPDILRVFPGDEERS
                     LLYLKVAAATEGTETPGAAMPIGADPLTSDQLEAVRLWIRGGAAEDGIVGGTLELLGC
                     EGMFDPEPNKINPLPAPAVDEGVQFYSGAWDLDAESEDEVCFATYYDFSDQVPADYQV
                     DCPELGQGRKCFAFRRNELAQDGQSHHSIIDIFTPSSDPNGGDWKNWECLGGATSGQP
                     CDPTDFGACGERGQCATRPESKVACVGYSNAPSNFAAGGGFGGGSATTVSLSGAQEST
                     RIDEPPVGVYSLLPLSGFVSWNSHGFNLTKQPTSIEQWVNLTFASEDERQYVRRSIFE
                     SGRIFSMSPVAPFEKREVCMTWTLPQHSRLLTLSSHMHARGELFRIWLPPNTPCEGVD
                     TCEPRNSAADYESRFYDDPAYAYYDPPLEYSSDAPAERTFLACAVYDNGADNPFDVKR
                     ESTKPKTPSCDHPFGQCGCPPSQRACFGGDNEGMSCGGDDSVCGEGGMCDACPLLGGV
                     TTDDEMFLPLGSYYVAEP"
     gene            complement(2677..3951)
                     /locus_tag="VFG22_02130"
     CDS             complement(2677..3951)
                     /locus_tag="VFG22_02130"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6415066.1"
                     /translation="MRRSFVKVVVSSVLVCWALAFVAIVLYARNQSWTDARAQGDGVF
                     LAYELLGQVPAQSRVERLQELRENFSIDLTLISLDEVQRRVGRPVSPGEQIPQRVSPR
                     EEWYFLVFEDGDGALAAGPVHPAIPPGVLPVGLILVLVGLPLVAGLIALRVEKQLTKV
                     ERASQALAVGDLSVRVDNPHGSPSELAASFNAMAERVERLIRSRDELVQAVSHELGSP
                     LSRLRFHMELLENQSDAEREKRLDAMTRELDELDELVRELLGYVQSDELELDRQAFDP
                     NRGLSDLAELARLEASEDRVVEVGLAVPSGASVFADQRLFQRAVENILRNAMKYARGN
                     VLLELTQDDEHVQVVVHDDGPGIPEELREKVMIPFFRLETDRDRKTGGAGLGLAIVNR
                     IMHRHGGRLAIDSSPLGGAMVTTWWPTLQASR"
     gene            complement(3948..4655)
                     /locus_tag="VFG22_02135"
     CDS             complement(3948..4655)
                     /locus_tag="VFG22_02135"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6415067.1"
                     /translation="MQTDKARLLIVEDDDQLAELLIEYLGQQGFELSHVASGDVAAEE
                     ILKTKPDLVILDLMLPGENGLDVCRQVRDSYSGAIVMLTASQSEADHVAGLELGADDF
                     VNKPIEPRVLLARIRSQLRRLNGGWASNDHDEDGILRVGTLQVDIASRDVSVEGQLVP
                     LTTMEFNVLLRLAIDAGSVVKRDELYTQVMGLEYDGIDRGMDVHVSRIRRKLQRSGFD
                     SSRLKSVRGVGYLLASG"
     gene            4800..5324
                     /locus_tag="VFG22_02140"
     CDS             4800..5324
                     /locus_tag="VFG22_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415068.1"
                     /translation="MTRHALPFFLFVLAISGGVGASAARAQDGLSGSWKLASSNAEAA
                     ERRAAIEAATQELPYFTRSRGRERLEERTTPLAKVRISVVGDRVDLTGSGPTISLTVG
                     GPSVPVEAEGRRGSARATRQEGNLVITLQGENGVRTTIYRLSEDGQRLVLDVDLAAKA
                     ISTPVRYRVTYERS"
     gene            5321..>5880
                     /locus_tag="VFG22_02145"
     CDS             5321..>5880
                     /locus_tag="VFG22_02145"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6415069.1"
                     /translation="MIPSHFSPQGYEEDDMIRWSLGVALIVWMTACSSSDETTPPLGS
                     ERTPQCVSYYDETLATEAPGAATWCREGAVMTWQSTNWPSPIELFYHCEGEPTAPAVY
                     LQHGWPTSSFDFQELSAALSSDFFVCALDTPGYGFSDKPDGYPYSILEDGEIVETFIE
                     TIMEVDEVLLLAHDKGDSVGLELLYR"
BASE COUNT         1116 a         1859 c         1815 g         1090 t
ORIGIN      
        1 ttggacgtgt tttgctcgac acctgtccgc gaaaccgttc gagaatcgtt tagaccaggc
       61 gagtcgcccg atattcgatt cccaccgcct ccaccaaaca agcgaagcaa ttgcgcacgg
      121 ttagcgaact ccgccatgga tcgcgtgacc acgtggcgac cgccttcggc tgtttgctgc
      181 ctcccttcgg tggatttcgc caacgttttg cgtgtgccgg gattggggtt gggtgtcctt
      241 acccagctcc accgtgcgct gggcgcgctc gaaggcacgg cgccgctcga ttgcggaaac
      301 gctggcgaag cggtactggg taaaggcgac atcgtaatcc tgaagaagat cgaggcaccg
      361 gacaggcgag aggtccaggc gctcgctcgc aaggtgcgaa gtcaggcccg caagcccgga
      421 gtcaagcgct ccgatatcaa gaaagcggtc cgcgcatccc gtcgtcgggg atgagagtcg
      481 tcctcgacac gaaggccctg atgtcaggca tcttcttcgc cgctcgcctg cgcgttggca
      541 ggtagcgctg cctgcgttgt gtctggggat tgcgctctcc tccgtgcctc tgggtacggg
      601 gggatcgagg taatgatgcc tagggagttc cagacgcggc tcctccacga gtgaacgccc
      661 cgacgacacc gtctcggagc aacacaggtt agagatcgca cgtacgacac gaagtcgtga
      721 ccaaacttgg agtcactgtg ctcaaggttc cgccacgtag tacgacccga gcggtagaaa
      781 catctcatcg tcggtggtga caccgccgag caggggacac gcgtcgcaca tgccgccctc
      841 cccgcatacc gagtcgtcgc ctccgcagct catgccttcg ttgtcgccac cgaagcatgc
      901 gcgttgtgaa gggggacaac cgcactgccc gaagggatgg tcacacgagg gcgtcttcgg
      961 cttagtcgac tctctcttga cgtcgaacgg gttgtcggcg ccgttgtcgt acacggcgca
     1021 cgcaaggaag gtgcgctcgg ccggagcgtc cgacgagtac tccagcgggg gatcgtaata
     1081 ggcataggca gggtcgtcgt agaaacggct ctcgtagtcc gcggcgctgt ttcgcggctc
     1141 gcaagtatct accccctcgc acggcgtgtt cggcggcaac caaatacgga agagctcacc
     1201 gcgtgcatgc atgtgggaac tcagggtcag aagccgcgag tgctgcggca aggtccaggt
     1261 catgcagact tccctcttct cgaacggtgc gaccgggctc atgctgaaga tccgacctga
     1321 ttcgaagatc gaacgcctga cgtactgacg ctcgtcttcg gatgcgaacg tgaggttgac
     1381 ccactgctcg atgctcgtgg gttgcttcgt gaggttgaac ccatggctgt tccacgagac
     1441 gaacccggag agtggtaaca atgagtacac accaaccggt ggctcgtcga ttcgcgtcga
     1501 ctcctgcgcg cccgagagag agaccgtggt cgcgctgcca ccgccgaagc cgccgccagc
     1561 tgcgaaattc gacggggcat tgctgtagcc tacgcacgcg accttgctct cggggcgggt
     1621 tgcacactga ccgcgttccc cgcaagcacc gaagtccgtt ggatcacagg gttggccaga
     1681 tgtagcacca cccagacact cccagttctt ccagtcaccc ccgttcggat ccgagctggg
     1741 cgtgaagatg tcgatgatgc tgtggtgcga ctgcccgtcc tgggcgagct cgttccgacg
     1801 gaacgcaaag cacttccgac cctggccgag ctcggggcag tcgacttggt agtcggcggg
     1861 cacttggtcg ctgaagtcgt agtacgtcgc gaagcagacc tcgtcctcgg actccgcgtc
     1921 gagatcccat gcccccgaat agaactgcac gccctcgtcg acagccggcg ccggcagggg
     1981 attgatcttg ttcggctccg ggtcgaacat gccttcgcag ccaagcagct ctagggtgcc
     2041 acccacgatc ccgtcttcgg cagcgccacc gcggatccag agacgaaccg cctcgagctg
     2101 gtcgctggtc agtgggtccg cgccaatcgg cattgccgcg ccgggtgtct cggtgccctc
     2161 ggttgcagct gcgaccttga ggtagagcag gctgcgctct tcgtcgccgg gaaacacgcg
     2221 aaggatgtcg ggatcgatca cgctgggcgt gcggacgagc gcctcgtacg aagcgtccgt
     2281 acgaagattg agatcgcctt ccatttgctc gccgtgacaa acatcggcgc tgcagccctt
     2341 cgcttcgaag atagtctctt ggatcgcggc gaaggtgctt gggtagtcgc aacccttggg
     2401 cgcggtagct tcagaggagg aatcggagcc gcacgcggat acgagactcg cgagcaggac
     2461 aaccgacgcg acggtcgaaa tacggagttg gggcatggtt caggagcttc ctagttggtc
     2521 gaagacaagc gtggacgttg acttggacga taggattgac ggggaggtcg agtgcgcgcc
     2581 agccattaac tgtagcgaaa agtagcgcag tttggagcgc agcctccttc aaaccgagat
     2641 tcgatgcccg ctccagaagg agaacttcaa gtacgtctag cgtgacgcct gaagagtcgg
     2701 ccaccacgtt gtgaccatcg caccgccgag cggagagctg tcgatcgcga ggcgcccgcc
     2761 gtgtcggtgc atgatccggt tgacgatggc caagccaaga cccgcccccc cagtcttgcg
     2821 atctcgatcg gtttcgagcc ggaagaacgg aatcatgact ttctctcgga gctcctcggg
     2881 aatgccgggc ccgtcgtcat ggacgaccac ctgcacgtgc tcatcgtcct gcgtgagctc
     2941 gagcagtacg ttcccgcgcg cgtacttcat cgcgtttctc agaatgttct ccaccgctct
     3001 ttggaagagg cgctgatcgg caaatacgct ggcgccgctg ggcaccgcca gaccgacctc
     3061 gaccacccgg tcttcggatg cttcgagtct ggctagctcg gcgagatcgc tgagaccgcg
     3121 attcggatca aacgcctgtc gatcgagctc gagctcgtcg gattgtacgt agcccagaag
     3181 ctcgcgaacg agctcgtcga gctcgtcgag ctcacgtgtc atcgcatcga gtcgcttttc
     3241 acgttcggcg tcagattggt tttcgagcag ctccatgtgg aatcgcagtc gcgaaagcgg
     3301 cgagccgagc tcgtgggaca ccgcttggac gagctcgtcg cgacttcgaa tcaatcgttc
     3361 cacccgctcc gccatggcgt tgaagctcgc cgctagctcg ctcggtgagc cgtgcgggtt
     3421 gtccacacgc acgctgaggt cgccgaccgc aagggcctgg cttgcgcgct cgactttagt
     3481 gagctgcttc tccacgcgga gcgcaattag gccggcgacc aagggcaacc cgactaggac
     3541 gagaatcagt ccgacgggaa gaacccccgg tggaatggcg ggatggaccg gaccggccgc
     3601 gagcgcgccg tcgccgtcct cgaacaccaa gaaataccac tcctcccgcg gggaaaccct
     3661 ctgcggaatc tgctcgccgg ggcttaccgg tcggccgaca cggcgctgca cctcgtcgag
     3721 tgagatgagc gtgaggtcga tcgagaagtt ctctcgaagc tcctgcaacc gctcgactcg
     3781 gctctgcgcg ggcacctggc cgagaagctc gtacgccaag aacacaccat cgccctgcgc
     3841 tcgtgcgtcc gtccacgatt ggttacgcgc gtaaagaacg atcgccacga atgcgagggc
     3901 ccagcagacc agcacgctac tcacgaccac cttcacgaag gatcgtctca tccgctcgcc
     3961 aagaggtagc ccacgccgcg cacggacttg agtcggctcg agtcgaagcc cgagcgttgc
     4021 agctttcggc gaatgcggct gacgtggacg tccatcccgc ggtcgatgcc gtcgtattca
     4081 agccccatga cctgggtgta gagttcgtcg cgcttgacca cgctcccagc gtcgatggcg
     4141 agccgtagga gcacattgaa ctccatcgtc gtgagcggga cgagctgacc ttcgacgctc
     4201 acgtcacggg atgcgatgtc gacctggagc gttcccactc tgaggattcc gtcttcgtcg
     4261 tggtcgttcg aggcccagcc gccgttgagc cgtcggagct gggagcggat ccgcgccaag
     4321 agcacccgcg gctcgatcgg cttgttcacg aagtcgtccg cgccgagctc caagccggca
     4381 acgtgatccg cctcgctttg gcttgccgtg agcatcacga tggctcccga gtacgagtcg
     4441 cgcacctgtc ggcagacatc gagaccgttt tctcccggca gcatcaaatc caaaatgaca
     4501 aggtcgggct tggtcttcag gatctcttcc gcggcgacgt ctcccgatgc cacgtgcgac
     4561 agctcgaacc cttgctggcc gagatactcg atcagtagct cggcgagctg gtcgtcgtct
     4621 tccacgatga gcaaacgtgc cttgtcggtc tgcattacac cctgcgcata gataagcaca
     4681 ttcaatcgcc aaagcgaggg actctacgct tcgcaacgaa acgcagcgaa gatgcaacag
     4741 acgcgcggcc cgacccgaag tacaaagcac acatgaccta caatcactga ggtgatccaa
     4801 tgacgcgaca tgcacttccc ttcttccttt tcgtactcgc catcagtgga ggcgtcggcg
     4861 cttccgcggc ccgcgcccag gacggactct ccgggagctg gaagctcgcc agctcgaacg
     4921 ccgaggcggc cgaacgacgc gcggcgatcg aggcggctac gcaagagctg ccgtatttca
     4981 cgcgttctcg cggccgtgaa cggcttgaag agcgcacgac cccgctggcg aaggttcgca
     5041 tctccgtcgt aggggaccgc gtcgacctga caggctcagg gccaacgatt tcgctcacgg
     5101 tcggaggacc gtccgtcccc gtcgaagccg agggccgccg cggcagcgcc cgggccaccc
     5161 gtcaagaagg aaatctcgtc atcacgctgc agggcgaaaa cggcgtccgg acgacgatct
     5221 accgactgtc tgaggacggc caacgcctcg tcctcgacgt ggacttggca gctaaggcca
     5281 tctcgacgcc ggtgcgctat cgcgtcacct acgagcggtc gtgattccct cgcacttctc
     5341 gccccaaggt tacgaggagg atgacatgat ccgttggtca ctgggagttg cattgatcgt
     5401 gtggatgacg gcctgcagct cgtcggacga gacaacgcct ccactaggaa gcgagaggac
     5461 accccagtgc gtttcttact acgacgaaac actcgcgacc gaggcccccg gcgctgcgac
     5521 ctggtgtcgg gagggtgcgg tcatgacgtg gcaaagcacg aattggccct cgccgatcga
     5581 gctcttctac cactgcgaag gcgagccgac tgctcccgcg gtctacctgc agcacggctg
     5641 gccgacgagc agcttcgatt ttcaagagct ttccgctgcg ctctcctccg acttcttcgt
     5701 gtgcgccctc gacacgccgg gctacggctt ctctgacaaa cccgatggct acccgtactc
     5761 gattctcgag gacggcgaga tcgtagaaac cttcatcgaa acgatcatgg aggtcgatga
     5821 ggtgctcctc ctggcacacg acaaggggga cagcgtgggg cttgagctgt tgtaccgcca
//
LOCUS       DASRZF010000048        13438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152 k141_34389,
            whole genome shotgun sequence.
ACCESSION   DASRZF010000048 DASRZF010000000
VERSION     DASRZF010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 13438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13438
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34389"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VFG22_02150"
     CDS             complement(<1..288)
                     /locus_tag="VFG22_02150"
                     /inference="COORDINATES: protein motif:HMM:NF040248.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415070.1"
                     /translation="MSPGLLRYLAGRLLRALLTVVGLVTIVFLLVRLIPGDPVEAILG
                     DQAGPEEKAELRRALDLDRPVGAQYLSFLGDMADGSMGTSFRQPDRSVWSMM"
     gene            complement(343..513)
                     /locus_tag="VFG22_02155"
     CDS             complement(343..513)
                     /locus_tag="VFG22_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415071.1"
                     /translation="MTGGFDANVPQDERIARIIDLSANQAEAKICVALAPGVNPDETT
                     GAPPEPELDGGL"
     gene            916..3015
                     /locus_tag="VFG22_02160"
     CDS             916..3015
                     /locus_tag="VFG22_02160"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HET6415072.1"
                     /translation="MGVGLAWLVGVSALAGDGLIRVASLVRSGDAQAALEETSRLPAR
                     TREEDGVRYLEGRLLLDIGRPCDAMDKLARTASTLPPSMRADSLRRWAIAAAQCGHCE
                     EARPILLTWASSESELTRHDRAVAADCAVQLGELDAAAEELSKLTKRKRGLPNRVALL
                     ALLSEVYVQLGRSEDARAAALEAWTTAVLPGEQAAAKQLEERALPSLADRISRAEEMM
                     DARRFSAAVEELEAIEPNDESEIDARWQHVYGMALFSMRTRYLDAARVLHQSAQRGGP
                     YETEDAFHSARALSRADQDARAIRAYRRFAQQYPRSKRAPEARFLAAWLEIRLGRAQG
                     EAQMERLLRGKSQVRGRWRRSVLWELGFRALETRRFGRARQYLSEYAAVATDSMDKAR
                     GFYWLGRSYRRGPKAVEAYLGAIAVEPLHWYAVLAAGRLKRLGVTPPEPFANASSPSS
                     VEVPSSFLAVPETFEAYRRLGLDLDGIAWLEAHERELVADFPSVQRIPLLTALYRDAG
                     AYREALTTARRRMAYLNSDPATHRWWWDAAYPMPWLSVVDENRGELPRALIYATMRQE
                     SGFRPEVVSRAGAVGLMQLMPDIATKLAGRPVSRTMLRAPETNIALGLKEMEALAGEF
                     NGVYPLSIAAYNAGKSRVRRWLKESRRMELDRFVERIPFNETRNYVRRVTTHYARYSY
                     LDDPESGWPDLPAFVNP"
     gene            3126..3899
                     /locus_tag="VFG22_02165"
     CDS             3126..3899
                     /locus_tag="VFG22_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HET6415073.1"
                     /translation="MNFIAAGLSDVGLQRDHNEDSYCILSEHRLFVVADGMGGHRAGD
                     VASRTAIAEMTAFFEASGRDSDGAGWIPPEDRRLTPEQNRLVSAVKRANQRIFQASIR
                     NRSVQGMGTTVVGALFEREDRKIHIAHVGDSRAYRIRAGDITQLTRDHSLLNDYLLVM
                     PNLSEAQRQRLPSNVITRALGMQDGVAVDLHFEKVEPGDVYVLCSDGLNGMVSDDRIL
                     ALVNSAGSDVELTARALISQANQNGGEDNITVVVIRITE"
     gene            3902..4396
                     /locus_tag="VFG22_02170"
     CDS             3902..4396
                     /locus_tag="VFG22_02170"
                     /inference="COORDINATES: protein motif:HMM:NF022161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 family protein"
                     /protein_id="HET6415074.1"
                     /translation="MAKTIAIRQLIDCSDRDFWERIFSSEDFNRYLYDGLGFEYELQE
                     WDLETGHRRAKVWPTHQMPRAVARVLGERFSFVEEGLYDASEQRYDFRVIPSTLADRI
                     RATGSVLIEAVSAHQCERRVTLTIGADVLGLGRLIETYFLAATREQYAKNAALVNEYL
                     ASHH"
     gene            complement(4393..6111)
                     /gene="ggt"
                     /locus_tag="VFG22_02175"
     CDS             complement(4393..6111)
                     /gene="ggt"
                     /locus_tag="VFG22_02175"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HET6415075.1"
                     /translation="MKARVSILICCVSLLLVAAFATAEPSTRSEHSAVFRQDAVAADQ
                     AEASRAGADILAAGGNAADAAAATMLALGVVNPASSGLGGGGFALYYDAATGTVTFID
                     FRERAPRAATRSMFDSEGEQTEGPASSASQLGGLAAGVPGEPAGIETLVKRFGNLSLR
                     EVIAPALRLADEGFTVSESLAAMGSAFGVQLRKDPVMASWDLSPGATLRRPALARVLR
                     AFAEKGASVIYGGEIGREMVAAVQKHGGIMSLDDLYAYRVRVREPLETEAFGYRWVTA
                     PPPSAGGFTMIQSLSILEQAAGPEPVYGAPLLHAMAESWKGPFLDRQRYFGDPDQVIL
                     PLDRMMSPGRIAKRAALAAGDRAHASSVYDFPIEWTSTEVASPENAGTSHLCVVDGEG
                     NVAAVTTTVNLPFGARFTAGGIVMNDEMDDFASGIGIANAFGLLGGARNLPGPWKRPV
                     STMSPTIVFEDGKPVLCVGASGGSRIVTATQQVAMNVLLHGMSAGDAVAAPRIHHQGV
                     PETLRVETVAPLDSVLQDALRAMGHKIEPIHNVANVQAIQIEQSGDRILRAASDPRKG
                     GRPAGH"
     gene            6159..7106
                     /gene="gshB"
                     /locus_tag="VFG22_02180"
     CDS             6159..7106
                     /gene="gshB"
                     /locus_tag="VFG22_02180"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HET6415076.1"
                     /translation="MHFVFVMDPVSTVIVDEDTSFALMLEAQARGHRVDHCLASDVGL
                     RGRRVVARVRRATMRLDRNEPITLGDPEDIDLVDVDAVFIRKDPPFTDAYLWLTLVLD
                     HLEGSTLVVNDPRGLREANEKLYAHHFPEIVPATIMTSHKDEILRFVSDVGGKAVIKP
                     IDGHGGEGVFLLAEGDSNLNALVEIVTHLGRRNAIVQALIPEIYDEGDKRILLVDGEL
                     LGVVGRVPSKGDLRSNVHVGGSAAPAEITDANRKIIETIRPRLAADRLFFVGLDVIGG
                     KLIEVNVTSPTLLQQMSRLSGKNLSAPVIDRLEEKVRAF"
     gene            complement(7122..7475)
                     /locus_tag="VFG22_02185"
     CDS             complement(7122..7475)
                     /locus_tag="VFG22_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415077.1"
                     /translation="MLINLYVLSLVLGAALLGASLFLNEKRVDADDEIRAHDDTTRPS
                     QFGQLDGIAGGDYFARTIRSRRFWTFFVSFFGMTGLVLDGLDLMSPIVSLIVAIAVAG
                     VAGAGASAAIRIGSS"
     gene            complement(7492..8424)
                     /locus_tag="VFG22_02190"
     CDS             complement(7492..8424)
                     /locus_tag="VFG22_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415078.1"
                     /translation="MRPGGASAPGKALLCGEYAVLEGAPAIVAAVNRRVSVRWTEGSQ
                     PMPPEVRATLHHARASLGPLEGSLIVDVSELQRDQIKIGLGSSAAAAAATAAAAYAKL
                     GRDLAGPATLREVFACALEGHASIAPQGSGVDVAASTYGGFLRFARKGNVIENRPLRL
                     PEGLQIRLVWTGHAARTSELLSQVHELRRDDPERYAACMERLSELSGQFASAFEADRP
                     EKILATAASYSTAMGALGEAAGAPILDARLRHAAELAARFSGAAKPSGAGGGDVAIAF
                     FLDAGAARGFELACKDEGLHPIDVSWGAPGVRAC"
     gene            complement(8421..9410)
                     /gene="mvaD"
                     /locus_tag="VFG22_02195"
     CDS             complement(8421..9410)
                     /gene="mvaD"
                     /locus_tag="VFG22_02195"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HET6415079.1"
                     /translation="MSERALEASARACANIALAKYWGKADLKWNVPAVPSISLTLDQL
                     VTETRVHFDPSLRADLVRLDGRRASTKEAERVVAMLNRVRSESDLRLKARVTSHNRFP
                     TAAGLASSASGFAALAAAASAAAGLRFNARHLSALARASSASAARSIYGGFVELPAGE
                     RGDSNLAARPIAAPNHWNLRLVVALTDPGRKKVGSTEGMERSRKTSPYYQAWIEQAPK
                     WSRKIKRAIKERDLDTLGAAMELSTLAFHCCAMTSDPPVLYWAPATLAALGTVRELRQ
                     RGVSAWATMDAGPHVKALCQAGDAERVRHALARTPGVTRTWVAKPGPGVEVYE"
     gene            complement(9407..10303)
                     /gene="mvk"
                     /locus_tag="VFG22_02200"
     CDS             complement(9407..10303)
                     /gene="mvk"
                     /locus_tag="VFG22_02200"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HET6415080.1"
                     /translation="MGFGRGKVILLGEHAVVHGVPAIAVGIERGATARAFPAKSDLLR
                     IAPWGLALAPDPSNEEPLARAFAAALALYPERPALEVNAEVELPAGAGLGCSAAIGVA
                     VLDAIDESLGIERSRMELGAAALAWEKVFHGNPSGIDNTISAVGGVGLYRKGLGLEPL
                     HSNKTLHLVIGYSGVSSSTKEMVASVARQLEHDPTRVKKAFEGIEVLVRNAKLAIEAG
                     DLVALGQLLDLNHSILTSLMLCTSRLDEMCQSARRAGALGAKMTGSGGGGCMFALAAK
                     RDEALRLRGVLGEGAFVAEVST"
     gene            complement(10303..11340)
                     /locus_tag="VFG22_02205"
     CDS             complement(10303..11340)
                     /locus_tag="VFG22_02205"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET6415081.1"
                     /translation="MAQGQMDLDSWMGAIRRDVDAILERFFAAKVEEARLLSPKAIEL
                     VTEVRDLTMRGGKRLRPIVTAAAYRAASGAEDVSPTFEVGASLELLQTYLLIHDDWMD
                     EDNERRGGLSVHYAFQKKHGNEHLGASLGILAGDLASTFGWEILLGTSFPAGRRDDGL
                     ALFVKIQKEVFAGQQLDLMADADVERMHDLKTGSYTTRGPAELGGVIADADPSAMQAL
                     RDWSAPLGVAFQLRDDLLGTFGTVEQTGKPGDDLRHGKHNAVVAAFRRLRPSEAENAA
                     LDAVWGRADASDDALRAATDVLDAAKSQVEERLAFLADRARSALAASPFDEGARRLLQ
                     EVTDRLTDRGN"
     gene            11402..12442
                     /gene="fni"
                     /locus_tag="VFG22_02210"
     CDS             11402..12442
                     /gene="fni"
                     /locus_tag="VFG22_02210"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HET6415082.1"
                     /translation="MSGDIEKRKADHLDLCATDEVSFRQRTTLLECVKLVHQSLPEAS
                     FDSIDTRVRLLGKELRAPILIAAMTGGHERAAEINQALASVANELGYAFGLGSQRAMQ
                     KRPETSWTYQVREHAPDVLLLGNVGVVQARDLSTKVIADMMTEVGADALCVHMNPAME
                     LVQPEGDRDFSRCIETFRRLWAELDAPIIAKETGNGISREVAARLRSIGIRHADTSGA
                     GGTSWVGVETLRAEGESRALGEALWDWGIPTAASVRYCVDAGMTTIATGGIRGGLDVA
                     RAIALGASCAGIARSVFQAYLEGGIDGARAFLSHIERELRAVMLLCGAKTIEDLQRAP
                     RIISGELRDWID"
     gene            complement(12443..>13438)
                     /locus_tag="VFG22_02215"
     CDS             complement(12443..>13438)
                     /locus_tag="VFG22_02215"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HET6415083.1"
                     /translation="RIGSGLGRAEVGALMSKLRCSVLVLVLLSSVATPLSAQLLEPTS
                     QQSTEADSRAEKAPSEPADQRAERTGEDPKPEGREDRNRLLILFLSLLGLGIVLFILE
                     RARSWISSNFPLQRVLGALVLVLRLVGLGLLLAIVVASVPGGHDWILWALAVLGLVVG
                     WSVRDLFADVVAGAVLAAERRVKKGMWVSGDGFQGTVEGRSLRATWLRDGQGHRLTVP
                     NRAMVGAPMVYDAGADAEHEVVVRLEGYEHAGKVRMALTDAILSSPWVLAGATPVVLR
                     DPADPVVWRVRTKLLEPRFSVHFEGELLERVEDLLRYIPEPQERETVPSEAPAGR"
BASE COUNT         2513 a         4277 c         4228 g         2420 t
ORIGIN      
        1 catcatcgac caaaccgaac gatcgggctg ccgaaacgag gtacccatgc ttccgtccgc
       61 catgtcaccg agaaagctga ggtactgcgc tccgacaggg cggtcgagat cgagcgctcg
      121 gcgaagctca gccttttctt caggaccggc ctgatcgcct aggatggcct ctaccgggtc
      181 accagggatg aggcggacca gcaggaacac gatcgtcacc agaccgacca cggtgagcag
      241 cgcccgaaga agccggcccg ccagataccg caagaggccc ggagacatca cgggaaccat
      301 actacgcccg accggtcgcg tcggcttacc tgccgtcgcg cgctagaggc cgccgtccaa
      361 ctcgggctcg ggcggggctc cggttgtctc gtccggattc acgcctgggg ccaacgcaac
      421 gcagattttg gcctctgctt ggttggcgct gaggtcgatg attcgggcga tccgctcgtc
      481 ctgaggcacg ttggcatcga agccgccggt catcgtgatc tattcgggca atacacagga
      541 gtattcggtc ctgcaattgc cggagttgtt gcagattttc atgcaccagg tctcggcggt
      601 gcggctcggg atgtatcgcc actcgacaca ggcgccctgg gggcaggtgt cgggatcaca
      661 tgagaagacg gtgcagtagc ccccgggttg ggcgaggtcg cactggcgtt caccggtggg
      721 ggagcaatcg cccgagttgc cgcagctgtc tccgatattc tgtttgcacc cccagagacc
      781 cagggtggca agcaataaga tagtcgcgct tcgcatcgtc agcggcggag catacgggac
      841 gcatcggacg atcgccaatc cctctgcacg acgacgaatg agtgatagat tccctacgga
      901 ttgcgacgtt tttgcgtggg ggtaggactg gcatggctgg ttggcgttag cgcgcttgcc
      961 ggggacggac tgatccgcgt tgcttccttg gttcgcagcg gagacgcgca ggccgcgctc
     1021 gaagagactt cacggctgcc cgctcgaacg agagaagagg atggggtgcg gtacctcgaa
     1081 gggcgtctgc ttctcgatat tggccgaccc tgcgatgcga tggacaagct tgcgcggacc
     1141 gcgtcgacgt tacctccttc gatgcgtgcg gattccctgc gccgctgggc gattgcggcg
     1201 gcccagtgtg ggcactgcga agaagcgcgc ccaattctat tgacttgggc gtcgagcgaa
     1261 tcagagctca cccgacacga ccgtgcagtc gccgccgatt gcgcggtcca actcggtgag
     1321 ctcgacgcag cggcagagga gctttcgaag ctcacgaaac gcaagcgcgg cctgcccaat
     1381 cgcgtggccc ttcttgccct tctttcggaa gtctatgtcc agctcggacg ctcagaagat
     1441 gcacgcgccg cggcactcga agcctggacg acggctgttc tcccgggcga gcaggcggct
     1501 gccaagcaac tcgaggagcg tgctctaccg agcctagccg accgcatcag ccgtgctgaa
     1561 gagatgatgg acgcacgccg cttttccgcg gccgtcgagg agctcgaggc gatcgaaccc
     1621 aatgacgaga gcgagatcga cgctcgatgg cagcacgttt acggcatggc gctgttcagc
     1681 atgcggaccc gctatctcga tgctgctcgc gtgctgcacc agtcggcgca gcgcggcggg
     1741 ccctacgaga ccgaggatgc gtttcactca gcccgcgcct tgtcgcgcgc cgatcaggac
     1801 gcccgcgcga ttcgagcgta ccggcgattc gctcagcaat acccgcgaag caagcgcgcg
     1861 cctgaggcac gatttctggc cgcctggctc gagattcggc tcggccgggc gcagggcgag
     1921 gcccagatgg agcgtctttt gcgcgggaag tcgcaggtgc gcggccgctg gcgacgatcg
     1981 gtgctctggg agctaggctt ccgtgctctc gaaactcgcc gcttcggccg agcgagacag
     2041 tacctttcgg agtacgctgc ggtcgccacc gattcgatgg acaaggcccg agggttctat
     2101 tggctcggcc ggtcctatcg cagaggtccc aaagcggtcg aagcgtatct gggcgccatc
     2161 gcggtcgagc cgctgcactg gtacgcggta ctggcagccg gccgtctcaa acgcctgggc
     2221 gtgaccccgc ccgagccgtt tgcaaacgca tcttcaccga gctccgtgga ggttccttcg
     2281 agctttctcg cggtccccga aacgttcgag gcttacaggc ggctcggcct cgacctggac
     2341 ggaatcgcct ggctcgaggc tcatgaaagg gagctggtcg ccgacttccc cagcgtccaa
     2401 cgcattccgc tgctgaccgc tctctatcgc gatgccggtg cgtatcgtga ggcattgacc
     2461 actgcacgcc ggcgcatggc gtacctcaat agcgacccag cgacgcatcg ttggtggtgg
     2521 gatgctgcgt atccgatgcc atggctctcg gtcgtcgacg agaatcgcgg cgagcttcct
     2581 cgtgctctga tctacgccac catgcgccag gagagtggtt tccggccgga ggtggttagc
     2641 agagcgggtg cagtcggctt gatgcagctg atgcccgaca tcgcaaccaa gctcgcaggt
     2701 agacctgtgt cgcggacgat gcttcgcgcg cccgagacga acatcgccct cggattgaaa
     2761 gaaatggaag cacttgccgg cgaattcaac ggtgtctatc ccttgagcat cgctgcttac
     2821 aacgctggca agagtcgcgt gaggcgctgg ttgaaagaga gtcgaaggat ggagctcgac
     2881 cgtttcgtgg agcgcatccc attcaacgag acgcgcaact atgtgcgccg cgtgaccacc
     2941 cactacgcgc gctattcata cttggatgac cccgagtcag gctggcccga tctccctgcc
     3001 ttcgttaacc cctgatttgc tccactgcga cgattcgata aactgtcgtc aacgatttgg
     3061 gttgcccgtc acggggtgga aggccaatag aaggtcgaat cacctcgcca ggcgtttgaa
     3121 tacgaatgaa cttcattgct gcagggctct ctgacgttgg acttcagaga gatcacaacg
     3181 aagacagcta ctgcattctg tcggagcatc ggctgttcgt cgttgccgac gggatgggcg
     3241 gccatcgtgc aggtgacgtt gcaagtcgca cggcgattgc ggaaatgact gccttcttcg
     3301 aagcgagcgg ccgcgacagc gatggagccg gatggattcc tccggaggat cgtcgtctca
     3361 cacccgagca gaaccgtctc gtgtcggcgg tcaagcgagc caaccagcgc atctttcaag
     3421 cctcgattcg gaaccgttcc gtccagggca tgggcactac ggtcgtcggc gctctcttcg
     3481 agcgggaaga tcgcaagatt cacattgcgc acgtcgggga cagccgggcg tatcgaattc
     3541 gcgcaggcga tatcacgcag ctgacccgcg atcactcctt gctgaacgac tatttgctgg
     3601 tcatgcccaa tctctccgag gcgcaaaggc agcgcctccc tagcaacgtg atcacccgtg
     3661 cgctcggaat gcaagacggc gtggccgtcg atttgcattt cgagaaagtc gagcccgggg
     3721 atgtctacgt actctgctcg gacggtctca atggaatggt gagcgacgat cgtatcctgg
     3781 ccctcgtcaa cagcgctggg tcggacgtgg agttgacagc aagggccctg atttcccaag
     3841 cgaaccagaa cggtggcgag gacaacatca ccgtggtcgt cattcgaatc accgagtagc
     3901 catggcgaaa actatcgcga ttcggcaact gatcgactgc tcagatcgag atttctggga
     3961 gcgaatattt tccagcgagg acttcaatcg atacctctac gacggactcg gcttcgaata
     4021 cgagcttcag gaatgggatc tcgagactgg tcatcggcgc gccaaagtgt ggccgacgca
     4081 tcagatgccg cgcgcggtcg cgagggtctt gggcgagcgt ttcagcttcg tcgaagaggg
     4141 tctctacgac gcttcggagc aacgctatga tttccgagtc attccttcga ccttggcgga
     4201 ccgaattcga gcgaccggaa gcgttctgat cgaagccgtt tcggcgcatc agtgcgagcg
     4261 gcgcgtgacg ctcacgatcg gtgcggacgt gctcggactg ggccggctca tcgagacgta
     4321 tttccttgca gcgacgcgcg agcagtatgc gaagaacgct gctctagtga atgaatacct
     4381 cgcgagtcat cactagtgac ccgcgggccg ccctcctttt cgggggtcgc tggcggcacg
     4441 aaggattcga tcgcccgact gctcgatctg gatcgcctgg acgttggcga cgttgtgtat
     4501 gggctcgatc ttgtgcccca tggcgcgaag ggcgtcttgt agaaccgaat cgagtggtgc
     4561 aaccgtctct acgcgtagtg tctccggaac gccctggtga tgaatgcgcg gagcggccac
     4621 cgcgtcgcct gcgctcatgc cgtgaagcag gacattcatc gccacttgct gcgtggcggt
     4681 gacgatccgg ctgcctcccg atgcgccgac gcatagaacg ggtttcccgt cttcgaagac
     4741 gattgtcggc gacatcgtcg agacgggacg cttccaaggt cccggcaaat tgcgcgcgcc
     4801 tccgagcaat ccaaaggcgt tggctattcc aatgccactc gcgaagtcgt ccatttcgtc
     4861 gttcatgacg atgcctccag ccgtgaaacg tgcgccgaat ggaagattca ccgtcgtcgt
     4921 gactgcggcc acattgcctt ccccatcgac cacgcagagg tgtgaggttc cggcattctc
     4981 gggcgaggcg acttcggtgc tcgtccattc gattgggaag tcgtagacgc tactggcgtg
     5041 cgcacggtcg cccgctgcga gggcagcgcg cttggcgatt cgcccggggc tcatcattcg
     5101 atcgagcggg aggatgactt ggtccggatc cccgaaatag cgttgccgat cgaggaaagg
     5161 acccttccac gattcagcca tcgcatgcag gagtggggca ccgtacaccg gctcgggtcc
     5221 cgcggcctgc tccagaatcg agaggctttg aatcatcgtg aagcctccag cgctcggagg
     5281 tggcgctgtc acccaacgat atccgaaagc ctccgtctcc aacggttcgc gcactcgaac
     5341 gcggtaggca tagagatcgt ccaaggacat gatgccgccg tgcttctgga ctgcggcgac
     5401 catctcgcgg ccgatctctc caccgtaaat gaccgacgcg cctttctcgg cgaaggcgcg
     5461 aagcacccgt gccagggcgg gacggcgcaa ggtcgcgcca ggcgagaggt cccagctcgc
     5521 catcactgga tccttacgca gttgaacgcc aaaggcgctg cccatggcgg cgagggattc
     5581 gctcacggtg aaaccctcat cggcgagtcg aagcgcgggc gcgatgacct cccgcaaaga
     5641 aaggttcccg aaacgtttga cgagagtctc gatccccgct ggctcaccag gtacacccgc
     5701 tgcgagcccg cccaattgag acgctgagct cgctggcccc tcggtttgct cgccctcaga
     5761 gtcgaacatc gagcgggtcg ccgcacgcgg ggcccgctcc cggaagtcga tgaacgtgac
     5821 agtgccagtc gcggcgtcgt agtaaagcgc gaagcctcct ccaccgagtc cgctacttgc
     5881 cggattcacg acgccgagtg caagcatcgt tgcggcagca gcgtctgcag cgtttccacc
     5941 cgcagcgagt atgtcggcac ccgctcgcga cgcttcagct tggtcagcgg caacggcatc
     6001 ctgcctgaac accgcagagt gctctgatcg ggtgctcggc tccgcggtcg caaaagccgc
     6061 gactagaagc agcgaaacac aacaaatcag gatcgaaacc cttgctttca tggccccagc
     6121 ttgcctccga tccggcctgg atgctaccgc aaggtctcat gcatttcgtg ttcgtgatgg
     6181 accccgtgtc gacggtcatc gtcgatgaag acacaagctt cgcgctgatg ctcgaggcgc
     6241 aggctcgcgg gcatcgagtg gatcactgtc tggcgagcga tgtggggctc aggggacggc
     6301 gcgtcgttgc ccgagtccgc cgagcgacga tgcgcctcga tcgcaacgag ccgatcactc
     6361 ttggcgatcc cgaggacatc gacttggtcg atgtcgacgc cgtgttcatc cgcaaggacc
     6421 cgcctttcac cgatgcgtat ctgtggttga ccttggtgct cgatcatctg gaggggagca
     6481 cactcgtcgt caacgacccg cggggtctgc gtgaagccaa cgagaagctc tacgcccatc
     6541 atttccccga gatcgtgccc gcgacgatca tgacgagcca taaggacgag atcctccggt
     6601 tcgtgagcga cgtcggcggc aaggccgtca tcaagccgat cgacggtcat ggtggcgagg
     6661 gcgtgtttct cttggccgaa ggcgacagca atctgaacgc gctcgtcgag atcgtcaccc
     6721 atctcggcag acgaaacgcc atcgttcagg ctctgattcc cgaaatctac gacgagggcg
     6781 acaagcgcat ccttctggtc gatggcgagc ttctcggtgt cgtcggacgt gtcccgagta
     6841 agggagatct gcgaagcaac gtgcacgtag gaggctcggc ggctccggcc gagatcaccg
     6901 atgccaaccg taagatcatc gaaaccattc ggcctcgatt ggcagccgat cgattgtttt
     6961 tcgtcggtct cgacgtcatc ggcgggaagc tcatcgaggt caacgtcacc agcccgactc
     7021 tgctccagca gatgtcgcgg ctctccggga agaatctctc ggcgcccgtg atcgaccggc
     7081 tcgaagaaaa agtgcgagcc ttctgagctt cggaaagcgg ctcagctaga gccgatccgt
     7141 attgccgcag aagcgccggc gcccgcaacg cctgcgaccg cgatggccac gatcagcgat
     7201 acgatcgggc tcatcaggtc caaaccgtcg agaacgagtc cggtcatccc gaagaaggaa
     7261 acgaagaacg tccagaaccg tcgtgagcga atggttctgg cgaagtaatc acctccggcg
     7321 atgccatcga gctgtccgaa ctggctagga cgcgtggtgt cgtcgtgagc acggatctca
     7381 tcgtcggcgt cgactcgctt ttcgttcaag aagagagagg cgccaaggag agcagctccg
     7441 agcacgagtg acaagacgta tagattgatc agcatctggc gcctctcctc ttcagcaggc
     7501 ccgtacgcct ggagcacccc aggagacgtc gataggatgc aatccttcgt ccttgcaggc
     7561 cagctcgaat ccacgcgcgg cgccagcatc gagaaagaat gcgatcgcca cgtcgccgcc
     7621 acccgcgccg gatggcttcg cagctcccga aaaacgggcc gcaagctcag cggcgtgccg
     7681 gagccgtgca tcgagaatag gcgcgccagc cgcttcgccc agcgcgccca tggcagtcga
     7741 gtaagaggcc gccgtggcca gaatcttctc ggggcggtcg gcctcgaagg cggaggcgaa
     7801 ttgaccggac agctccgaga gccgctccat gcaggcggcg tagcgttccg gatcatcgcg
     7861 ccgaagctca tgcacttggc tgagcagctc gctggttctc gcggcatggc ctgtccagac
     7921 cagacggatt tggagtcctt ctggaagtct gagagggcgg ttctctatca cgttcccttt
     7981 gcgagcgaag cgaagaaacc cgccataggt cgaagccgca acgtcgactc cactgccctg
     8041 cggagcaatc gaagcgtgac cctcgagcgc gcaggcaaag acttctcgca gcgtcgcggg
     8101 accggcgagg tcgcggccca gcttggcata cgcagcggcg gcggttgcag cagcggcggc
     8161 ggcggaagaa ccgagtccga tcttgatctg atctcgttgc agctcactca cgtccacaat
     8221 caacgacccc tcgagcggcc ctagcgacgc acgggcgtgg tggagggtcg cgcggacttc
     8281 tggaggcatc ggttgcgagc cctccgtcca gcgaaccgag acccgccggt tgaccgcggc
     8341 aacgatcgct ggtgcacctt cgagcactgc atattcaccg cagaggagcg ccttccccgg
     8401 cgcgctcgcg cccccgggtc tcattcatac acctcgacac cgggtcccgg tttggcgacc
     8461 caagttcgcg tgacgcccgg ggttcgagcg agcgcgtgcc tcacgcgctc ggcatcgcca
     8521 gcctggcaga gcgctttcac gtgcggccca gcatccatgg tcgcccaggc cgaaacaccc
     8581 cgctggcgaa gctcacggac ggtacccagc gctgcgagcg tcgccggggc ccagtagaga
     8641 accggcggat cggaggtcat ggcgcagcaa tgaaacgcga gcgtgctcag ttccatcgcc
     8701 gcacccaggg tgtcgagatc gcgttccttg atcgcacgct tgatcttgcg cgaccatttc
     8761 ggggcctgtt cgatccaggc ttgatagtag ggggaggtct tgcgcgagcg ctccattccc
     8821 tcggtcgatc ccaccttctt cctgccgggg tccgtgagcg cgaccaccag acgaaggttc
     8881 cagtggttcg gcgctgcgat cggtctggca gcgaggttgg agtcgccccg ctcccccgcc
     8941 ggaagctcca cgaacccccc atagatcgat cgtgcagccg acgcgctcga cgcccgggcg
     9001 agggcgctca agtggcgtgc gttgaatcga agccctgccg ctgcagaggc tgccgccgca
     9061 agcgctgcga accccgaggc gctcgacgcg agcccagcag cggtcggaaa gcgattgtgg
     9121 ctcgtaacgc gcgctttcag acgcaaatcg gactcactcc gaacgcggtt caacatggcg
     9181 accacacgct cagcctcttt cgtgctcgca cgacgtccgt cgagacgcac caggtccgcg
     9241 cggagcgacg ggtcaaagtg aacgcgtgtt tccgtgacga gctggtcgag ggtgagcgaa
     9301 atgctcggca cggccggcac gttccatttg aggtcggcct tcccccagta cttggccagc
     9361 gcgatgttcg cgcaggcccg cgcgctggct tccaaagcgc gttcgctcac gtgctcacct
     9421 ccgctacgaa agcgccctcg ccgagcacgc cgcgcaagcg caaagcttcg tcgcgtttcg
     9481 ccgcgagcgc gaacatgcag ccgcctccgc ccgagcccgt catcttcgcg ccaagcgctc
     9541 cagcgcggcg tgccgactgg cacatctcgt ccaaacggct ggtacagagc atcagggagg
     9601 tcagaatcga gtgattcaag tcgagcaatt gtccgagggc aacgagatcg cccgcttcga
     9661 tcgcgagctt tgcgttgcgc accaagacct ctatcccctc gaatgccttc ttcacccgcg
     9721 taggatcgtg ttccagctga cgcgcgaccg atgcgaccat ttccttagtg ctcgaggaca
     9781 cgccgctgta accgatgacc aggtgcaggg tcttgttcga atgcagcggc tcgaggccaa
     9841 gacccttgcg atatagcccg acgcccccga ccgccgagat cgtattgtcg atacccgacg
     9901 ggttgccgtg aaacaccttc tcccaggcca gcgcagccgc tccgagctcc atccgcgaac
     9961 gctcgatccc gagtgactcg tcgatcgcgt cgaggacggc cacaccgatc gcggccgagc
    10021 aacccaaacc tgcgcctgca ggcaactcga cctcggcgtt cacctcgagg gccggccgtt
    10081 ctggatagag cgcaagagcc gccgcgaacg cccgtgcgag cggctcctcg ttcgaagggt
    10141 ccggggcgag agccagaccc cagggcgcga tccggagcag atcgctcttt gccggaaacg
    10201 cgcgcgccgt agcgccgcgt tcgattccca ccgcgatcgc cggtacgccg tgaaccaccg
    10261 catgctctcc gagtagaatg accttgcccc ggccgaagcc catcagtttc ctcggtccgt
    10321 gagtcggtcc gtgacttcct gcagcaagcg tcgggcgccc tcatcgaacg ggctcgccgc
    10381 aagcgcgctc cgtgctcggt ccgccagaaa agccagcctc tcttcgacct gggacttcgc
    10441 cgcatcgagg acatccgtgg ccgccctcag cgcgtcatcg cttgcatcgg cgcgtcccca
    10501 gacagcgtcg agcgctgcat tctcggcttc actcggccgg agacggcgaa acgcggcgac
    10561 caccgcattg tgctttccgt gccgcaggtc atcgccgggt ttaccggttt gttcaaccgt
    10621 tccgaaggtg cctagaaggt cgtcgcgaag ttgaaaggca acccctaggg gcgcggacca
    10681 gtcgcgaagg gcttgcatcg cagacgggtc tgcatctgcg atcacgcctc cgagctccgc
    10741 tgggccgcgc gtggtgtagc ttcccgtctt gaggtcgtgc atgcgctcga cgtcggcgtc
    10801 ggccatcagg tcgagttgct gcccggcgaa gacctccttt tgaatcttca cgaagagggc
    10861 cagaccgtcg tctcgccggc ccgcggggaa gctggtgccg agcaagatct cccagccaaa
    10921 ggtggatgcg aggtcgccgg ccagaattcc gaggcttgcg cccagatgtt cgtttccgtg
    10981 cttcttctgg aacgcgtagt ggacagagag cccgcccctg cgctcgttgt cctcgtccat
    11041 ccagtcgtcg tgaatcagca ggtaggtttg caggagctcg agcgaagccc ccacttcgaa
    11101 cgtcgggctc acgtcctccg ctccgctggc ggcgcggtat gcggcggctg tgacaatcgg
    11161 gcgtaggcgc ttacctcccc gcatcgtcag gtcccgtacc tccgtgacaa gctcgatcgc
    11221 cttgggcgag agcagacggg cctcctcgac cttcgctgcg aaaaaacgct cgaggatggc
    11281 gtccacgtca cggcggatag ctcccatcca ggagtcgaga tccatttgcc cttgcgccac
    11341 ggtgtctgtg tccttattcc gagctacgta cacggcgaga cgctgccgag caagcaatgc
    11401 gatgagcggg gatatagaga agagaaaggc agatcatctg gatctgtgcg cgaccgacga
    11461 agtctcgttc cggcaacgga cgacgcttct cgagtgcgtg aagctcgtcc accaatccct
    11521 tcccgaggcg agcttcgatt cgatcgacac gcgggttcgc ttgctcggca aggagctgcg
    11581 cgcgccaatc ttgatcgcgg cgatgaccgg cgggcacgag cgcgcagcgg agatcaacca
    11641 ggccttggcc tcggtggcga acgagctcgg ctatgcgttt ggcttgggga gtcagcgcgc
    11701 gatgcagaag cgcccggaga cgagttggac gtaccaggtg cgcgagcatg caccggacgt
    11761 gcttctcttg ggaaatgttg gcgtcgtgca agcgcgcgat ctgagcacca aggtgatcgc
    11821 cgacatgatg accgaggtag gggccgacgc cctttgcgtg cacatgaacc cggcgatgga
    11881 gctggttcag ccagaaggtg atcgagactt cagccgctgc atcgagacgt ttcgacgtct
    11941 ctgggccgag ctcgatgcgc cgatcatcgc caaagagacg gggaatggaa tcagcaggga
    12001 ggtcgccgca cgtcttcgat cgatcggaat tcggcacgcg gatacatcgg gtgctggggg
    12061 cacatcctgg gttggcgtcg agaccttgcg cgcggagggc gaatcgcgag cactgggcga
    12121 ggctctctgg gactggggga tcccgactgc agcatccgtt cgctattgcg tcgacgcagg
    12181 catgacgacg attgccaccg gcggcattcg gggcggactc gacgtggccc gagcgatcgc
    12241 tctcggcgcg agctgcgcgg gaatcgcgcg cagcgtcttt caggcttacc tcgagggcgg
    12301 catcgatggg gcgcgcgcgt ttctttccca catcgagcgc gagcttcgag ccgtgatgtt
    12361 gctctgtggc gccaaaacca tcgaagacct tcagcgggct ccacgcatca tttcaggaga
    12421 gctgcgcgac tggatcgact agtcagcggc cagcgggagc ttcgcttggg acggtctcgc
    12481 gctcctgggg ctcagggatg tatcgcagca ggtcttcgac gcgctccaga agctcgcctt
    12541 cgaagtgcac cgaaaaacgt ggttccagca acttcgtccg gacgcgccag acgaccgggt
    12601 cagctgggtc gcgcaagacc acgggagttg cgcccgcgag cacccaggga gagctgagaa
    12661 tcgcgtctgt gagcgccatt cgcaccttgc ccgcgtgctc atagccctcg agcctcacca
    12721 cgacctcgtg ctcggcatcg gcgccggcat cgtacaccat cggcgcgcct accatcgccc
    12781 ggttgggcac ggtcagacgg tgtccttgcc cgtcccgcag ccaagtcgcg cgaagcgatc
    12841 gaccttcgac ggtcccctga aatccgtcgc cagacaccca catccccttt ttcacgcgcc
    12901 tctcggcggc aagaaccgcg ccggcaacca cgtccgcaaa gagatcgcgg acggaccagc
    12961 caacgaccag cccgaggaca gctagagccc aaagaatcca gtcatgaccg ccggggaccg
    13021 acgcaacgac gatcgcgagt agcagaccca aaccaaccag ccgcagaacg agcacgagcg
    13081 cacccagcac tcgctgcagc ggaaagttgg agctgatcca gctgcgtgca cgctccagga
    13141 tgaacagcac gatcccgagg cccagcaagc tgaggaatag gatcaacaga cggtttcggt
    13201 cctcacggcc ctcgggcttc gggtcctcac cggtccgctc tgccctctgg tctgcgggtt
    13261 cggacggcgc cttctcggcc cgactgtctg cctcggtcga ctgctgcgat gtgggttcca
    13321 gcagctgtgc tgaaagaggc gtggccaccg agctgagaag cacgagcacg aggactgagc
    13381 aacgaagttt agacatcaga gcacccacct ccgctcggcc aagacccgac ccaatacg
//
LOCUS       DASRZF010000049        13204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4509071, whole genome shotgun sequence.
ACCESSION   DASRZF010000049 DASRZF010000000
VERSION     DASRZF010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 13204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13204
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4509071"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="VFG22_02220"
     CDS             <1..421
                     /locus_tag="VFG22_02220"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET6415084.1"
                     /translation="LLVGLVMSIATWGLYPISVDFVPAIGVEVPKLYGLLRQFPGPVW
                     AFPVLVLVVAAEELVWRGLAIDLFSKSLGRSGAVFLASLVYVLPQIAFRSPLLVIVAL
                     SCGLVWGGLRVRTGGLLAPLVAHLTWDLLVFVLFPVE"
     gene            complement(481..2292)
                     /locus_tag="VFG22_02225"
     CDS             complement(481..2292)
                     /locus_tag="VFG22_02225"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET6415085.1"
                     /translation="MPERFETDVLIVGAGPAGMVSALCLAKLGVRSTIIERYPGVNPH
                     PKAHELNARSIEILGELGVSMEELSREAAPDADGSRILFCKTINEELGRIDLLADGGS
                     AQKYARHLRSDKPYLNLSQTELELVIREHVARDPHVELMVDHEWEGFEQTETEVLSRV
                     RSLAGSADTLLITSRYLIGADGAGSKTRASLGIEMIGPDRLQDFINAYFEIGLRDYVH
                     TPAKLYWILHPAAAGAFIAHHVDKRWTYNVPITAPYEKPEDFTEDVLKKRIEIALGTD
                     RYDIQIKSVSYWRMTAQVAERYRRGRVFLVGDSAHRFPPTGGLGMNTGIADAHNLGWK
                     LAAVLKGRSPESLLESYEIERKPVAERNGAESRANFDKIFEVIEAFGLSRDAPQLMAR
                     ISNHPFVKGMPGFLRRPLLGLLRAPAHFALGRFRWSPSLRNKVASSIEDQVSHFDRIG
                     LDIGYVYQEGAVVPEENAPPAAELQVMEYEPTTRPGARFPHVALGGGPNTGSTHDLIG
                     YDRYTLLIGAAGSGWSDALRRLNGSIETPVISVSLGDVSADTGFEGIARLEAACEIES
                     EGALLIRPDGHVGWRHRKASADPDGTLLAAFRNLKID"
     gene            complement(2512..3147)
                     /locus_tag="VFG22_02230"
     CDS             complement(2512..3147)
                     /locus_tag="VFG22_02230"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HET6415086.1"
                     /translation="MSANSKRTASEGATPKSQQTRERIVRAALDAFATYGFDGASTRQ
                     IAALAGENQGLITYHFSTKETLWKAAVDSIFLEFRRELAERMLVLVDADVRTQLRLLL
                     IFFVRHAARRPEQMRLMIQEGKADNARMQWLVEHHVKDAFGIFGSIIDNAIEEGILPE
                     APVVHYFYILVGACSLLFTVAPECQYLTGDNPLDPAMVEAHAEAVVNLVLR"
     gene            3285..4001
                     /locus_tag="VFG22_02235"
     CDS             3285..4001
                     /locus_tag="VFG22_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415087.1"
                     /translation="MEQTPHGWLILDKDAGVLAYTYRFGSGSANAFVARYDGDKLLVL
                     SPPHRLSEGAFADLEVFGTVESVVANNGFHHLGQAEWKKRYPLASFYASPAAAKRIKK
                     KNPEAGDFEPLSQLSQKLGGNVGVIEAPADKCGETWAYAKTKKGYAWYASDTLANMPE
                     LPKALPFKLVLSLTKSAPGYRVFGLALKFMTSDKRDLLRAMQEALRTRPVAIMVPAHG
                     GILAHDRLAEETQRLIASAL"
     gene            complement(4099..4716)
                     /locus_tag="VFG22_02240"
     CDS             complement(4099..4716)
                     /locus_tag="VFG22_02240"
                     /inference="COORDINATES: protein motif:HMM:NF021485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2202 domain-containing protein"
                     /protein_id="HET6415088.1"
                     /translation="MLASRLGFVLAFVFAFGIAACSDAGVAPPESSLSSEEIMDLQYM
                     REEEKLARDVYLVLHDEWGLQIFSNIASSEQTHTDAVATTLERVEIEDPVVDDTIGVF
                     VDPRLAKLYQEMVDEGMSSAINALHVGATIEEVDMIDIQSAIDRAEAPAIIALYESLL
                     CGSRNHLRAFAAELESAGQPYVAQFLDASEVEAIVSSPREQCNTR"
     gene            complement(4830..5201)
                     /locus_tag="VFG22_02245"
     CDS             complement(4830..5201)
                     /locus_tag="VFG22_02245"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415089.1"
                     /translation="MVGISKALRIEAREYGVRVSVLCPGVIRTPILRAGEFGRTNLNA
                     EGERKMMAFWERLRPMDPRAFARKTLREVARNVPIIIVPSWWKVLWWVERLSPTLSSW
                     LWQKAYDRMRAELAQHRLELG"
     gene            complement(5224..5469)
                     /locus_tag="VFG22_02250"
     CDS             complement(5224..5469)
                     /locus_tag="VFG22_02250"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6415090.1"
                     /translation="MATKTAFITGGASGIGRALAEELAERGVELVLADRQLALAEEVA
                     SSIRRAGGRATAHELDVRDPAQFQRVRRGALACAECW"
     gene            5656..6390
                     /locus_tag="VFG22_02255"
     CDS             5656..6390
                     /locus_tag="VFG22_02255"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET6415091.1"
                     /translation="MHKASWVFLLAVSLVLTAGCKKDELRAKDARIAELEALGQDTGT
                     SLDQCKKDRENLNKLLKGKAGEIDAMEAALAEAAAREAQAAKRLAEYNSMLKQLKSMI
                     DSGKLKVRIVRNKMVIELPEAVLFPSGSAKLKPEGVRVLAELGPVLASIQDRQFQVGG
                     HTDNKPIRTARFPSNWELAGARAIDVTQLLIEYGLPGTRVSAAAYADTQPVADNDTPE
                     GRALNRRIEIALQPNLDELPDLSSFE"
     gene            6494..7162
                     /locus_tag="VFG22_02260"
     CDS             6494..7162
                     /locus_tag="VFG22_02260"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HET6415092.1"
                     /translation="MSIKVHHLNYSRSLRVLWLLEELELDYTIQTWERDASFRAPASA
                     AEIHPLGRFPMVEVDGRVLAESGAVLEYFADREGKLRPNDEAEKLEQTFFLHYAEGSL
                     MPPLLVQLILTRLRAAPMPFFIKPIARGIADQIHKGYSGPALDLHFGFVEQTIAQRPY
                     FAGKTFSIADIQMIYPVEAGLSRAAGERPNMQAWLERMKSRPAYQRAEAKGGAAVPPA
                     KSGA"
     gene            7271..8416
                     /locus_tag="VFG22_02265"
     CDS             7271..8416
                     /locus_tag="VFG22_02265"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HET6415093.1"
                     /translation="MSSNPSVHRHLVRQLTEVYRAPAVPVIGDFRIPVTVYTDASKFE
                     AERKLLRRLPIPLAHVSQVASPGSCLVHDALGVPIIVTRNRQGELRAMLNVCRHRGAR
                     LLEQEGFCQVRKGFRCHYHGWTYDTDGKLVHVPNEALFSSLDKETRPLVGLPVHERFG
                     LIWVVATPGERYDFDSFLDPLEDDLAPLDLESHVVFRRATATRRTNWKIVMEAFLDSY
                     HVEHLHKKTVAPYFLGNRSYTEQAGPHVRSIVGRTGFSEAVESGQVEEIRDIATPTYV
                     IFPNTIFVVQPGFVSRATAYPVGIDEIYWEHDLIIPEDPRTAKAKAHWELNFELIQNG
                     VFSGEDLWICEQIQRGLASGANEHLIYGTEEAPIRWWHEELERRLCT"
     gene            complement(8432..11557)
                     /locus_tag="VFG22_02270"
     CDS             complement(8432..11557)
                     /locus_tag="VFG22_02270"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET6415094.1"
                     /translation="MKITDLFIRRPVIAIVVNLVIVIAGIQAIRSLTVRQYPRSENAA
                     ITITTAYVGANAELVRGFITTPIERAIAAADGIDYISSESRQGVSIIRANLDLNYDAV
                     RALSEIGAKVDQVRGDLPPEAEIPVLDVVSVDSQFASAYLSFSSDFLKQNEITDYLVR
                     AIQPRLSAVDGVQRAEILGARTFAMRVWLNPERMAAHKIGPAQVRNALAANNYLAAVG
                     QTKGALIQVNLTTNTDLESVEEFKRLVVREQDDAIVRLEDIAEVALGAVSYDDQVNFT
                     GATAVFIGVWVLPNSNALDVVKRVRTELEAIREELPEQLRAEIAYDATKYIDSAIREV
                     VTTLSETLAIVIVVIFLFLGSFRAVIVPVVAIPISLIGAVFLMQLFGFTINLLTLLAI
                     VLSVGLVVDDAIVMVENIERHLREGMSRTESALKGARELVGPVIAMTITLAAVYTPIA
                     FQGGLTGALFQEFALTLAGAVLISGVVAITLSPVMSASFLPDEKNLRGLPARIDRDFG
                     RLKDVYLRVLDRTLNARPAIYLAWVVLSGLAAVLFVMSPRELAPNEDQGVIFGVVNTP
                     ANSTLGQLAPSVKAVHAMAMSYPETEFTFQITTPGGGFWGMGLKPWDERTRTAFELMP
                     LVGADVNAIPGIQTFPVLPPALPGGGEFPVEFVVASTAESVDILQFVQELQKKAAMSG
                     MFAFPPLIDVKIDQPESEITFDRDKVAELGLSLQAVGQELGSAVGGNYVNRFSIAGRS
                     YKVIPQLERSARLNADQLQDIYVTKPDGDLIALSTFATVKDRVVPRSLNRFQQLNSVK
                     LSGVAIRPLDEALTFLETEAKTILPRGYSVDYTGESRQLRKEGNKFVPAFALAVVLIF
                     LVLAAQFNSFRDPLVILGGSVPLAMFGASIFTFLKMPDPNMHYWTDGWTTTLNVYSQV
                     GLVTLIGLVSKNGILIVEFANELQREGQAKLEAVRNAAAVRLRPILMTTTATVLGHFP
                     LTLVTGPGAEARNSIGLVLVGGMAIGTIFTLFFVPSIYMLFAKDRSASTSADAVGPAP
                     AAPAAPAPAAG"
     gene            complement(11576..12712)
                     /locus_tag="VFG22_02275"
     CDS             complement(11576..12712)
                     /locus_tag="VFG22_02275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET6415095.1"
                     /translation="MKRNKKPILLAVGVIVLVVLVLGGIKASQIRAMIAAGEAFVPPQ
                     QAVTTAEVTEVSWKPELTAVGSVVAVQGVTVASEVPGTITEIAFKSGQMVKTGDLLVR
                     LDTSIERAQLASAVASAKLADIDYQRKKNLPPTAAVSAADVDAAAAKASQAAADVANF
                     KAVIARKTIRAPFSGRVGIRDVDLGEVLQPGTPIVSLQSSDPIYVDFSLPQQVLSLIE
                     PGHTATISTDGFPDRTWEGTVEVIDTEVDIGTRNFDVRAIVANPDGKLRTGMFVDVTV
                     VQPEARELLAIPASAVLFAPYGDSVYIIKEQESESGEKQLIAEQVFVRLGERRGDLVA
                     VSSGLAVGDAVVSTGAFKLQSGMAVTIRNDLAPEINTDPNPPNE"
     gene            complement(12858..13172)
                     /locus_tag="VFG22_02280"
     CDS             complement(12858..13172)
                     /locus_tag="VFG22_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415096.1"
                     /translation="MRASRIQCGAVLCGLLAPLLLPLPGYGQETVTTEPPSYQSASPP
                     PSEAPCPKGKRGGAIAGIVVGSVFWYVLPMSIPVLITQTKKLKAHNRAQLERRCQYAY
                     PR"
BASE COUNT         2488 a         4018 c         4202 g         2496 t
ORIGIN      
        1 tctgctggtc gggctagtca tgtcgatcgc gacctggggg ctctacccca tcagcgtcga
       61 tttcgtcccc gcgatcggcg tggaagtgcc gaagctctac ggcctccttc ggcagtttcc
      121 cggcccggtt tgggcatttc cggttctagt attggtcgtt gccgcggagg agctggtctg
      181 gcgcggcctg gccatcgacc tgttttcgaa gtcgttaggc cgctcgggcg cggtcttcct
      241 cgcctcactg gtatacgtgc ttccgcagat cgccttccgc tcgccgcttc tggtgatcgt
      301 cgcgctgtct tgcggtctag tttggggcgg cctgcgcgtg cggaccggtg ggcttctcgc
      361 gcccttggtc gcccatttga cgtgggacct gctggtcttc gtgctgttcc cggtcgagta
      421 gtcaccaaga cggcgggcgc cgcagggccg ggaggcgatt ccgacgatcg gcctctccgc
      481 tcagtctatc ttcaagtttc ggaaggctgc caagagggta ccgtctggat cggccgacgc
      541 ctttcggtgc cgccagccca cgtggccgtc aggacggatc aacagtgcac cttcggattc
      601 gatttcacag gctgcctcaa gacgagcgat gccctcgaag ccggtgtcgg cgcttacgtc
      661 gccaagcgac acggagatca ccggagtctc gatcgacccg ttcaggcggc gtagcgcgtc
      721 gctccagcca ctcccggccg caccgattag aagtgtataa cggtcgtagc cgatgaggtc
      781 atgtgtcgaa ccggtgttcg gacccccacc gagggcaacg tgcgggaacc gagcgccagg
      841 ccgagtcgtg ggctcgtact ccatcacctg aagctccgct gcgggaggtg cattctcttc
      901 gggtaccacg gctccctcct gatagacgta gccgatgtcg aggccgattc gatcgaagtg
      961 cgaaacttga tcttcaatgc tcgaagccac tttgttgcgg aggctcgggc tccatcgaaa
     1021 acgccccaat gcgaaatggg ccggtgcgcg gagcagcccc aagagcggac gccggaggaa
     1081 acccggcatg cccttcacga aggggtgatt gctgatccgt gccatgagct ggggtgcgtc
     1141 tcgactgagg ccgaaggctt cgatgacctc gaagattttg tcgaagttcg cgcggctctc
     1201 ggctccgttg cgttcggcga cgggcttgcg ctcgatctcg tagctctcga ggagggactc
     1261 cggagagcgg cccttcaaga ccgccgcgag cttccaaccg agattgtggg catccgcgat
     1321 ccccgtgttc atcccgaggc cgcccgtcgg gggaaatcga tgagctgaat cgccaacgag
     1381 aaagacacgt cctcgacggt aacgctccgc aacttgggct gtcatacgcc agtacgaaac
     1441 cgacttgatc tgaatgtcgt accggtcggt gccgagggcg atttcgattc gcttcttcag
     1501 aacatcctcg gtgaagtctt ccggcttctc gtacggagcg gtgatgggaa cgttgtacgt
     1561 ccatcgtttg tccacgtggt gcgcgatgaa agcgcccgcg gccgcgggat gaaggatcca
     1621 atagagcttc gcgggtgtat gcacgtagtc ccggagaccg atttcgaaat acgcgttgat
     1681 gaagtcctgc agtcgatcgg ggccaatcat ctcgatcccg agcgacgcgc gggtcttgct
     1741 ccctgcgccg tcggccccga tcaggtagcg gctcgtgatc agcagggtat ccgccgagcc
     1801 tgccaaagac cgcacgcggc tcagcacctc cgtctcggtc tgctcgaacc cttcccattc
     1861 atgatcgacc atcagctcga cgtgagggtc gcgcgcgacg tgctctcgga tgacgagctc
     1921 gagctcggtc tgcgacaggt ttaggtaggg cttgtcggag cgcaggtgac gcgcatattt
     1981 ctgtgcactc ccgccgtcgg cgagcaggtc gatccggccg agctcctcgt tgatcgtctt
     2041 gcagaacaag attcgagacc catcggcatc gggcgccgcc tcgcgcgaca gctcttccat
     2101 cgagacgccg agctcaccga ggatctcgat cgaccgcgcg ttgagctcgt gcgccttcgg
     2161 atgtgggttc acgccgggat aacgttcgat gatggtgctg cggacgccga gcttggcgag
     2221 gcaaagcgcg ctcaccatcc cggcaggccc cgcacccacg atcaagacgt cggtttcgaa
     2281 tcgttccggc atggttcaag tcagcacgaa ggcgagcagg aagagcgcga tgaggttcgg
     2341 gcgcacttgg acgactcgga tggtgtggga aacggtcacg acaggctgtc cggggtgtta
     2401 ggcatacgta ttaaaatgag gccgtaagac ttgtcaatcg aataaataac tgatagccag
     2461 ggttcaaggc caaccacttc ctcttcgact tctcttacaa ctggcgatag gtcagcggag
     2521 gacgagattg acgaccgctt cggcgtgcgc ctcgaccatg gccgggtcga gtggattgtc
     2581 gccggtcaaa tactggcact cgggcgccac cgtgaacaag agcgagcagg caccgaccaa
     2641 gatgtagaaa tagtgaacga cgggcgcctc gggcaagatg ccctcttcga tcgcgttgtc
     2701 gatgatcgag ccaaagattc cgaacgcatc cttgacgtgg tgctcgacga gccactgcat
     2761 gcgagcgttg tcggccttgc cctcctgaat catcaaacgc atctgctccg ggcggcgtgc
     2821 tgcatgccga acgaagaaga tgagcaagag ccgcagttga gttcggacat cagcgtcgac
     2881 caacaccagc atgcgctctg cgagctcgcg ccggaactcg aggaagatcg agtcgaccgc
     2941 ggctttccaa agggtctcct tggtcgagaa atgatatgtg atgagcccct gattttcgcc
     3001 cgcgagcgcc gcgatctgtc gggtcgatgc cccgtcgaag ccgtaggtcg caaacgcatc
     3061 gagcgcagct cgcacgatcc gctctcgcgt ttgctgagat tttggcgtcg cgccttcact
     3121 ggccgtccgc ttcgagttcg cactcatgaa tccaaactcg caccaattta ttcgattgac
     3181 aaggtgacgc agcgaacagt acccggaccc tcacgcgggt tgccgaccta ggctaaagtc
     3241 caaagccacg ttgcatcgcc cgaccctcct cgataggcag actcatggaa cagacaccgc
     3301 acggatggtt gatcttggac aaggacgcag gtgttcttgc gtacacctac agattcggtt
     3361 ctggctctgc gaacgcgttt gtcgcgcgct acgatggcga caagcttctc gtgctgagcc
     3421 caccgcatcg actctcggaa ggcgcgttcg cggacctcga agtctttggc accgtcgagt
     3481 cggtggtcgc caacaacggc tttcaccatt tgggtcaggc cgagtggaag aagcgctatc
     3541 cgttggcgtc gttttacgcg tctccggctg cagccaaacg catcaagaag aagaatccgg
     3601 aggccgggga cttcgagccc ctgtcgcagc tttcgcagaa gcttggcggg aacgtcggcg
     3661 tgatcgaggc gcccgcggac aagtgcggag agacatgggc gtacgccaag acgaaaaagg
     3721 gctacgcctg gtacgcctcc gacaccttgg cgaacatgcc cgagcttccc aaggcactgc
     3781 cgttcaagct cgtcttgagc ttgacgaaga gcgcaccggg ttaccgcgtg ttcggcttgg
     3841 cgctgaagtt catgacatcg gacaagcggg acctcctacg cgcgatgcaa gaggcgcttc
     3901 ggactcgccc cgtcgccatc atggtgcccg cgcacggcgg tatcctagcg cacgacaggt
     3961 tagccgagga aacgcaacga ctcattgcat cagccctgta gctgcagtcg tcggcggcag
     4021 ccgaagttgg cggatagtga attcaagagg cctgcctggg acatccgaca tagcgatgag
     4081 ctctgcgcaa ttgggctctc agcgcgtgtt acattgctcg cgcggggacg agacgatcgc
     4141 ttctacctcc gaagcgtcga ggaactgcgc gacatagggt tgcccggcac tttcgagctc
     4201 ggccgcgaat gcgcgaaggt gattgcgcga tccgcacagc agtgactcgt agagcgcgat
     4261 aatcgccgga gcctcagcac ggtcgatggc agactgaatg tcgatcatat cgacctcctc
     4321 gattgtggcg cctacgtgca gtgcgttgat cgcagagctc atcccctcgt cgaccatttc
     4381 ctggtagagc ttggccagcc tcgggtcgac gaacacgccg atagtgtcgt ccacgactgg
     4441 gtcctcgatc tcgacccgct cgagggtagt cgcgacggca tcggtgtgtg tctgctccga
     4501 gctcgcgatg ttcgagaaaa tttgaaggcc ccactcgtca tggagcacca agtagacgtc
     4561 gcgcgcaagc ttctcttctt cgcgcatgta ctggagatcc atgatctcct cactactcag
     4621 cgagctctcg gggggagcaa ccccggcgtc ggagcaagcc gcgatgccga acgcaaatac
     4681 gaaggcgagc acgaacccaa gtctcgaggc aagcatgagc aaccttctag cctcacacct
     4741 cgagtggaga cttctgatag gtagcttggg tacgaattgc gaagctgcaa tgcggcatat
     4801 cgtcgcatga tcctcggata cgatgtctat cagccgagct ccagtcggtg ctgcgcgagc
     4861 tctgcgcgca tgcggtcgta ggccttctgc cacaaccacg agctcaatgt cggagagagc
     4921 cgctccaccc accagaggac cttccaccag gaaggaacga tgataatcgg cacgttgcgc
     4981 gcgacctcac gcagcgtctt gcgtgcgaac gcgcgcgggt ccatcggcct caaccgctcc
     5041 cagaacgcca tcatctttcg ctcaccctcc gcgttgaggt tcgttcggcc gaactcgccc
     5101 gctctgagga tcggcgtgcg aatcacgccc gggcagagga cggagacgcg aaccccatat
     5161 tctcttgcct cgatccgaag cgctttgctg atgccgacca ccgcgtgctt agtcgctgcg
     5221 tagctaccag cattcggcgc aggcaagagc cccgcggcgg acgcgctgaa actgggcggg
     5281 gtcacggaca tcgagctcat gggcggtggc tcgtccgcca gcgcgccgaa tcgagctggc
     5341 gacctcttcg gcgagcgcga gctgcctgtc ggcgaggacc agctcgacac ctcgttccgc
     5401 caactcttcg gcgagcgctc gaccgatgcc cgatgcgcca cccgtaatga aggctgtctt
     5461 ggtcgccatg gcttaccttt cgggctgttc gtcgaggaag aggaccgata agccgtgcga
     5521 tctcgtcttc cgtcggaccg cgcacgaggg gtttacggcc tgctttttcc gaccttccgc
     5581 agcgagttgc cttgtctctg cggcttggta gtgtgcgcca cccgtttttg gaaaacaatc
     5641 cttttggagg gacatatgca taaagcaagc tgggtatttt tgttggcggt cagtctggtg
     5701 ctgacggccg gttgtaagaa ggacgagctc agggcgaagg acgcccggat cgcggagctc
     5761 gaggccctgg ggcaggacac gggcacttcg ctcgatcaat gcaagaagga ccgggagaac
     5821 ctcaacaagc tcttgaaggg caaagctggt gagatcgatg cgatggaggc cgcgctcgca
     5881 gaggccgccg cgcgcgaagc acaagctgcg aagcgcctcg ccgagtacaa ctccatgttg
     5941 aagcagctga agtcgatgat cgactccggc aagctcaagg tgcgcatcgt gcgcaacaag
     6001 atggtcatcg agctaccgga agccgttctg ttcccgagcg gcagcgccaa gctcaagccc
     6061 gaaggcgtcc gcgtcctcgc ggagctcggt ccggtgctcg cgagcatcca ggaccgccag
     6121 ttccaagtgg gcggccacac ggacaacaag ccgatccgca ccgcacgctt cccgtccaac
     6181 tgggagctcg ccggtgcgcg cgcgatcgac gtgacgcagc ttctcatcga atacggtctt
     6241 cccggaaccc gggtctccgc tgccgcatac gctgacaccc aaccggtcgc ggacaacgat
     6301 acccccgagg gccgcgcgct caaccggcgt attgagatcg cgctccagcc gaacctggac
     6361 gagcttccgg acctctcgag cttcgagtaa accgagaagc gctgcacaca ttgaacgtgc
     6421 cgagatgcat tgcgcactcg gcacgtttca attgaagtgg ggttggagca gccttcgatg
     6481 ctacggtcgc accatgagca tcaaagtcca ccacctcaac tactctcgct ccttgcgtgt
     6541 tctctggctc ctcgaagagc ttgagctgga ctacacgatc caaacctggg agcgcgacgc
     6601 gagcttccgg gcgccggcgt cggccgctga gattcatccg ctcggacgct ttccgatggt
     6661 cgaagtcgac ggccgcgtgc tcgcagaatc aggcgcggtg ctggagtact tcgcggatcg
     6721 ggagggcaag ttgcggccga acgacgaggc cgagaagttg gaacagacgt tcttccttca
     6781 ctatgcggag ggctcgctca tgccgccgtt gctggtccag ctcatcctga ccaggctgcg
     6841 ggctgcgccc atgcccttct tcatcaagcc catcgcgcgc ggcatcgcag accagattca
     6901 caagggctat tcgggaccgg ctctcgatct tcacttcgga ttcgtcgagc agacgatcgc
     6961 gcaacggccc tacttcgccg gcaagacatt ctcgatcgcg gacattcaga tgatctaccc
     7021 ggtggaggcc ggcctatccc gagcggcggg cgagcgtccc aacatgcagg catggctcga
     7081 gcggatgaag agccggcccg cctaccagcg cgcagaggcc aagggcggcg ccgcagtccc
     7141 ccccgccaag agcggtgcgt gaagctctgg ctagtcacgt tggctttcga ggccgaacgg
     7201 cccgattctg gtgagcgtgc agggctgcgc ggcattcgcg cacccccagt tcgtgtatga
     7261 tggcttcggc gtgtcgagca atcccagtgt ccatcgtcat ctcgtccgtc agctgacgga
     7321 agtctatcgg gcgccggcgg tccccgtgat cggcgacttc agaatcccgg tgacggtcta
     7381 cacggatgca tcgaagttcg aggcggaacg aaagctactg cgccgactac cgattccgct
     7441 ggctcacgtt tcgcaagtgg cttcgcccgg ctcatgcctc gtgcacgatg cgctgggcgt
     7501 tcccatcatc gtgacgcgca atcggcaagg ggagctgcgt gcaatgctca acgtgtgccg
     7561 gcaccggggg gcgcgtctat tggagcagga gggtttctgc caggtgcgca agggctttcg
     7621 ctgccactac cacggttgga cttacgacac ggatggaaag ctggtgcacg ttccgaacga
     7681 agcgctcttc tcgtcgctgg acaaggagac gcgcccgctg gtcggtttgc cggtgcacga
     7741 gcgtttcggc ttgatctggg tcgtcgcgac gcccggcgag cggtacgact tcgactcatt
     7801 tctcgatccg ctcgaggacg acctagcgcc cctggacctg gagagccatg tggtgtttcg
     7861 acgcgccacg gcgacccgaa ggaccaactg gaagattgtc atggaggcct tcctcgacag
     7921 ctaccatgtc gaacatctgc acaagaagac cgtcgcgcct tatttcctcg gcaaccgaag
     7981 ttacaccgag caagccggac cgcacgtgcg atccatcgtc gggcgtaccg gtttctcgga
     8041 ggcggtcgaa tcgggccagg tcgaagagat tcgagacatc gccaccccga cgtacgtgat
     8101 ctttcccaat acgattttcg tcgtgcaacc gggtttcgtg agccgcgcga ccgcgtatcc
     8161 cgtcggcatc gacgagatct actgggagca cgacctgatc atccccgagg atccgagaac
     8221 ggcgaaagca aaggcacact gggagctcaa cttcgagctg atccaaaacg gcgtcttcag
     8281 cggtgaagac ctctggatct gcgagcagat ccagcgaggc ctggcgagcg gcgccaacga
     8341 gcatctcatc tatggcaccg aagaagcgcc gattcgatgg tggcacgaag agctcgagcg
     8401 gcggctctgc acgtagaggg tctcgagccc gtcagcccgc agcaggggct ggcgcggcgg
     8461 gagccgcggg ggctggcccg actgcgtcgg cgctcgtact cgcgctgcga tcctttgcga
     8521 agagcatgta gatcgagggt acgaagaaga gcgtgaagat cgtgccgatc gccatgccgc
     8581 cgacgaggac gagaccgatg ctgttgcggg cctcggcgcc aggaccggtg accaatgtga
     8641 gcgggaagtg gcccaagacg gttgctgtgg tggtcatcag gatcgggcgc agacgaacgg
     8701 cggcggcgtt tcggactgcc tcgagcttgg cctgcccctc gcgctgcagc tcgttggcga
     8761 actcgacgat gagaatgccg ttcttcgaaa ccaggccgat cagggtgact aggcctacct
     8821 gcgaatagac gttcagcgtg gtggtccagc cgtcggtcca gtagtgcata ttcgggtcgg
     8881 gcatcttcaa gaacgtgaaa atcgaggcac cgaacatggc gagtggtacg gagccgccca
     8941 aaatcaccag tggatcgcgg aagctgttga actgggcggc gagcaccaaa aagatcagga
     9001 ccaccgccag cgcaaacgcg gggacgaact tgttgccttc cttccggagc tggcgcgact
     9061 cccccgtgta atcgacgctg tatcctcggg gcagaatggt cttggcttcg gtctccaaga
     9121 aggtgagtgc ttcatcgagc ggccgaatcg cgacgccgct gagcttgacc gagttcagtt
     9181 gctgaaagcg attgagcgag cgaggaacga cgcgatcctt gaccgtagca aaggtgctga
     9241 gggcgatgag gtcgccgtcg ggtttggtga catagatgtc ttgtagttgg tcggcgttga
     9301 ggcgcgcgga ccgctcgagc tgcgggatga ctttgtagct tcggcccgcg atgctgaagc
     9361 gattgacgta gttgccgccg accgccgagc cgagctcctg gcccaccgcc tgcagactga
     9421 ggccgagctc ggctaccttg tcgcgatcga aggtgatctc ggattccggt tgatcgatct
     9481 tcacgtcgat cagcggcggg aacgcgaaca tcccgctcat ggccgctttc ttttggagct
     9541 cttgcacgaa ctgcaggatg tcgaccgatt ccgcggtcga ggcgacgacg aactccaccg
     9601 ggaactctcc gccgcctggg agcgcgggcg gcaggaccgg aaaggtctga atcccgggga
     9661 tcgcgttgac gtccgcgccc accaagggca tcagctcgaa cgcagtccgc gtgcgctcgt
     9721 cccagggctt gagacccatg ccccaaaaac caccgcccgg cgtggtgatc tgaaacgtga
     9781 actcggtttc tgggtagctc atggccatcg cgtgcacggc tttgaccgag ggggcgagtt
     9841 ggccgagcgt cgagttcgct ggcgtgttga cgacgccgaa gatgacgccc tggtcttcgt
     9901 tgggggcgag ctcgcgtggc gacatcacga acaagaccgc agcgagcccg ctgagcacca
     9961 cccaggcgag atagatggcc ggccgggcgt tgagcgtccg gtcgagcacg cgcaagtaga
    10021 cgtccttcag gcgaccgaag tcgcggtcga tgcgcgcggg gagtccgcgc aagttcttct
    10081 cgtcgggcaa gaagctcgca gacatcaccg gggaaagcgt gatggcgacg actccggaga
    10141 tcaggacagc acccgcgagc gtcagggcga actcctggaa gagcgctccc gtcaatcctc
    10201 cttggaacgc gattggcgtg tagaccgcgg ccagcgtgat cgtcatcgcg atgaccgggc
    10261 cgacgagctc gcgcgctccc ttcagcgcgg attccgtgcg gctcatgccc tcacgcagat
    10321 gccgctcgat gttctcgacc atcacgatgg catcgtcgac gaccaaaccg accgacaaga
    10381 cgatcgcgag gagggtcagc aagttgatgg taaagccgaa gagctgcatc aggaacacgg
    10441 cgccgatgag cgagatcggg attgcgacca ccggcacgat aacggcgcgg aaagagccca
    10501 aaaagagaaa gatgacgacg atgacgatcg ccagggtttc gctcaaggtc gtgacgactt
    10561 cgcggatcgc gctgtcgatg tacttggtgg cgtcgtacgc gatctcggcg cgaagctgtt
    10621 cagggagctc ttcgcggatc gcctcgagct cggtgcggac tcgtttgacg acatcgaggg
    10681 cgttgctgtt gggcagcacc catacgccga tgaaaactgc cgtcgcaccg gtgaagttga
    10741 cctggtcatc gtagctcacg gctccaaggg cgacctcggc gatatcctcc aatcgaacga
    10801 tggcgtcgtc ttgctcgcga accacgagac gcttgaactc ttccacagat tcgaggtcgg
    10861 tgttggtcgt gagattgacc tggatcagcg cgcccttggt ctgaccgaca gcagcgaggt
    10921 agttgttggc cgcgagcgcg ttgcgcacct gtgcggggcc gatcttgtgt gccgccatcc
    10981 gttcaggatt gagccaaacg cgcatggcga acgtacgagc gcccaggatc tcggcgcgtt
    11041 gcacaccatc gacggcggag agacgcggct gaatggcgcg caccaggtag tcggtgatct
    11101 cgttctgctt gaggaaatcc gacgaaaagc tgagatacgc ggaagcgaat tggctgtcga
    11161 ctgagacgac gtcgagcacg ggaatttcgg cttcgggcgg gaggtcccct ctgacctgat
    11221 cgaccttggc tccgatttcg gaaagcgctc ggaccgcatc gtagttgagg tcgaggttcg
    11281 cgcgaatgat cgacacgccc tggcggctct ccgacgagat gtagtcgatg ccatctgcgg
    11341 cggcgatggc ccgctcgatt ggggtcgtga tgaagccgcg cacgagctcg gcgttggctc
    11401 ccacgtacgc agtggtgatc gtgatggccg cgttttcgct tcttgggtac tgcctgaccg
    11461 taagcgagcg aatggcctga ataccggcga tgacgatcac gagattgacg actatcgcga
    11521 tgaccgggcg gcggatgaac aggtcggtga tcttcacggt gcttcccggg cgcgactatt
    11581 cgttgggcgg attcggatcg gtgttgatct cgggcgcaag gtcgttgcgg atggtgactg
    11641 ccattccgct ctgaagcttg aacgcacccg tcgagacgac cgcgtcccct acagcgagac
    11701 cggagctgac agcgaccaag tccccgcggc gctccccgag gcgcacgaac acctgctcgg
    11761 cgatcagctg tttctcgcca gactcgcttt cctgttcctt gatgatgtag accgagtcgc
    11821 cgtagggcgc gaacaagacg gcgctcgcag gaatggcgag caattcgcgc gcctcgggct
    11881 ggaccaccgt cacgtcgacg aacatgccgg tgcggagctt cccgtcggga tttgcgacga
    11941 tcgcccgcac gtcgaagttg cgcgtgccaa tgtcgacctc agtgtcgatc acctctaccg
    12001 ttccctccca ggtcctgtcg gggaagccat cggtgctgat ggtagcggtg tgtccgggct
    12061 cgatcagcga cagcacttgc tgcggcagcg agaaatcgac gtagatggga tcggacgact
    12121 ggagggagac gatcggcgtc cccggttgca gaacctcccc gagatccacg tcacgtatcc
    12181 caacgcgacc cgagaatggc gcgcggatcg tctttctcgc gatcacggct ttgaagttgg
    12241 cgacgtccgc ggcggcctgg gaagccttcg cggcggcggc gtccacgtcg gccgcgctga
    12301 cggcagcggt aggaggcaga ttcttcttcc gttggtagtc tatgtcggcc agcttggcgc
    12361 tcgccactgc ggaggccagc tgcgctcgct cgatcgaggt atcgagacgc accagaaggt
    12421 cgcccgtttt gaccatctgg ccggacttga aggcgatctc ggtgatcgtg ccgggaacct
    12481 cgctggccac ggtcacgccc tgcactgcga cgacggagcc cactgcggtg agctcgggct
    12541 tccagctcac ctcggtcacc tcggcagtcg tgaccgcctg ctgcggcggt acgaacgctt
    12601 cgcccgcggc gatcatggcg cgaatctgcg aggccttgat gccaccgaga acgagcacga
    12661 ccagcacgat tacgcctact gcgaggagga tgggtttctt gttgcgtttc atcgacgaag
    12721 caaactgggt agccgaggaa ccggggctcg aaaggcaggc ctagcgctcg cgcggcggcc
    12781 gcacaactcc ccccatcgtg gttttctcag ctcgcgaagg cgcccgatgc gggaaaatct
    12841 cgcttggcgc tcgatcctca gcgaggatac gcgtactggc atcgccgttc gagctgggcc
    12901 cgattgtgcg ccttgagctt cttcgtttgg gtgatgagca cgggtatcga catgggcaag
    12961 acgtaccaaa acacgctgcc gaccacgatg cccgcgatcg ccccgcctcg cttccccttg
    13021 gggcatggcg cttccgacgg aggcggtgaa gcgctctggt aagaaggcgg ctctgtcgtc
    13081 acggtttctt gcccatagcc ggggagcggc aagagcaagg gggcgagtaa gccgcagagg
    13141 accgcgccac attggattcg gctagctctc atcttcacct ccgggcgagt ctatgtatgg
    13201 agcg
//
LOCUS       DASRZF010000050         3004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3820707, whole genome shotgun sequence.
ACCESSION   DASRZF010000050 DASRZF010000000
VERSION     DASRZF010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3820707"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="VFG22_02285"
     CDS             complement(<1..540)
                     /locus_tag="VFG22_02285"
                     /inference="COORDINATES: protein motif:HMM:NF019977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase N-terminal domain-containing
                     protein"
                     /protein_id="HET6415097.1"
                     /translation="MKITVEHLTEYRYAQPVGFGEHSLYLRPRESQTIHVESFSLQCE
                     PLGTVRWVRDCFNNVVAVVSFGLSRSALLSFDVRMAFEVAQENPFDFVLEDRAKQFPF
                     IYDEREKGALWPYLEPDVSARWPVLDWVRSSLGDLSSGRDTISFLTDLNRAINQNIAY
                     ERRDEEGTRTANETLTLRRG"
     gene            677..1681
                     /locus_tag="VFG22_02290"
     CDS             677..1681
                     /locus_tag="VFG22_02290"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6415098.1"
                     /translation="MKNVIRFPTQLRSARSSPLDGMRYRTIHGYRRAFIQAGAGPAVL
                     LIHGIGDSADSWLDLIPALARHHTVIAPDLLGHGDSDKPRADYSIGAYANAMRDLLSV
                     LDIERVTVVGHSLGGGVAMQFAYQFPERCERLVLVSTGGVSHEVHPMLRFASAPNADL
                     VLPLLGAPGSRLLGRAVFAMMKALRTRIGRDADQLSRVFDALPDAASRRAFIRTLRGA
                     VDWRGQAITMLDRCYLARDMPTLLIWGDRDGVIPVAHAYKVHAALPDSRLEVFTNCGH
                     FPHQNNPARFLRLFRDFYDSTSPCSHSAEEWRALLRKGSIAAPVAAPALRSVAPRRFV
                     "
     gene            complement(1620..>3004)
                     /locus_tag="VFG22_02295"
     CDS             complement(1620..>3004)
                     /locus_tag="VFG22_02295"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HET6415099.1"
                     /translation="HHLRRLPPPTSEKLESDLLELDLSNSLDENRTSTIISTRPLSGE
                     PWSRFVPGEMRVISRGHVIETGETIPPPPPQRAFDTHSSSQVDPEASQVGTRTQTSQV
                     DAGMSQVGKGEVGKGAGQRGAQMQRGGARLAGSCPDPGSRARAMPAPGLPALGKKVSS
                     HTYEVTHETIYRYETAVETSSHTVRLQPVRDLTQDVLEYKLEISPPGQAFSYEDVFGN
                     HVLSFQINQPYTELWVRAHSKVQIFHVDTHSALRTTNIPLVWMPWQRQMMNPFLLPPE
                     LPETELRELSGFAMSFAARREYDLLESLIDLNEALHRDFKYIAGATTLETSPFDVFQE
                     RRGVCQDFANLFVCLARLLGVPARYRVGYIHTGATYHNQIQSEASHAWAEVYLPSVGW
                     RGFDPTNGCLVDLDHIRVASGRNYRDATPTAGTLYAGGHGEKLEVSVSVKRIDEAPPS
                     EVQAQQQAQQ"
BASE COUNT          543 a          935 c          946 g          580 t
ORIGIN      
        1 gcctctgcgg agcgtgagcg tctcgttggc ggtacgggtc ccttcctcgt cgcggcgctc
       61 gtacgcgatg ttttgattga tggcgcgatt gagatccgtg agaaacgaga tcgtgtcgcg
      121 tccggagctc aagtctccca aggagctcct gacccagtcg aggaccggcc agcgtgccga
      181 aacgtccggc tcgagatacg gccacagcgc gcccttctcg cgctcatcgt aaatgaaggg
      241 gaactgcttg gcacggtctt cgagaacgaa gtcgaagggg ttttcttgcg cgacctcgaa
      301 cgccatccgg acgtcgaacg agagcagtgc cgagcgcgac aggccaaagc tcacgacggc
      361 caccacgttg ttgaagcaat cgcgcaccca gcgaaccgtg ccgagcggtt cgcactggag
      421 tgaaaaagac tcgacgtgaa tggtctgact ctcccgcggc cgcaggtaca gagaatgctc
      481 gccaaatccg acgggttgcg catatcgata ttcggtcagg tgctcgaccg tgatcttcat
      541 gggttcgggc ttagcaggtt tatcgctcga gagtgatttc aatctggttt ctccagcatg
      601 tctggggcga aacatcgtca cagaagcgtc gagcatgggt gcgaaatggt ctcggcaacg
      661 atattctgcg ctcacggtga agaacgtcat tcgctttccc actcagctac gcagtgcgcg
      721 ttcttcgccg ctcgatggaa tgcggtaccg gaccatccac ggttaccgtc gcgccttcat
      781 ccaggcgggc gccggccctg cggtgctcct cattcacggg atcggcgaca gtgcagacag
      841 ctggctcgac ctcattccag cgctcgcgcg ccatcatacc gtgatcgcgc ccgatctgct
      901 cgggcacggg gactccgaca agccgcgggc cgactactcg atcggcgcct acgccaacgc
      961 catgcgcgac cttcttagcg tgctcgacat cgaacgcgtc accgtggtcg ggcattcgct
     1021 tggcgggggt gtcgcgatgc agttcgccta tcagttcccg gaacgctgtg agcggttggt
     1081 gctggtgagc accggcgggg tcagccacga ggtgcatccg atgctgcgct tcgcctccgc
     1141 gcccaacgcg gatctcgtct tgccgctact tggtgcgccc ggttcgcgtc ttctcgggcg
     1201 ggccgtgttc gccatgatga aggcgctcag aacgagaatc ggacgcgatg cggatcagct
     1261 cagtcgtgtc ttcgatgcgc tccccgacgc ggcatcgcgc agagccttca tccgaaccct
     1321 tcgaggcgcg gtcgattggc gcggccaagc catcacgatg ctcgaccgct gctacctggc
     1381 gcgagacatg cccacactcc tcatctgggg cgatcgggac ggggtgatcc cggtcgcgca
     1441 cgcatacaaa gtccacgccg cattgccgga tagccggctc gaagtgttca cgaactgcgg
     1501 gcactttcct caccagaaca atcccgcgcg cttcctgcgc ctttttcgag acttttacga
     1561 cagcacgagc ccttgctcgc acagcgcgga ggagtggcgt gcgcttcttc gcaaaggttc
     1621 tattgctgcg cctgttgctg cgcctgcact tcgctcggtg gcgcctcgtc gattcgtttg
     1681 acgctcaccg aaacctcgag cttctctccg tgcccgccgg cgtagagcgt tcccgccgtc
     1741 ggcgtcgcgt ctcggtagtt gcgcccagaa gccacccgga tgtggtcgag gtcgacgagg
     1801 catccgttgg tcggatcgaa tccgcgccaa ccgaccgagg gcaagtagac ctcggcccag
     1861 gcatgtgacg cctccgactg aatctggttg tgataggtgg cgcccgtgtg gatgtacccc
     1921 acacgatagc gcgctggaac gccgagcagg cgcgcgaggc aaacgaagag gttggcgaag
     1981 tcctgacaga cgccgcgacg ctcttggaat acgtcgaagg gggacgtttc gagagtcgtg
     2041 gcgcccgcga tgtacttgaa gtcgcgatgc agcgcttcgt tgaggtcgat gagcgactcg
     2101 agcaagtcat actcgcgccg agccgcgaag ctcatcgcaa acccggagag ctcgcgaagc
     2161 tccgtctcgg gcagctcggg cggcaagagg aacgggttca tcatctggcg ctgccaaggc
     2221 atccacacga gcggaatgtt cgtggtgcgg agcgcggagt gcgtatcgac gtggaagatt
     2281 tggaccttgg agtgagcgcg cacccagagc tcggtgtagg gctggttgat ctggaagctc
     2341 agaacgtgat tgccgaatac gtcctcgtag gaaaatgcct gccccggagg agagatttcg
     2401 agcttgtact cgagcacgtc ttgcgtgagg tcccgaaccg gttggaggcg aaccgtgtgg
     2461 gacgaagtct caacagccgt ctcgtaccgg taaatcgtct catgcgtaac ctcgtacgtg
     2521 tgcgagctga ccttcttccc aagagcaggc aaccccggag ccggcatcgc ccgcgcgcgt
     2581 gaacccgggt ccggacagct ccccgccaac cgagccccac cccgctgcat ctgcgccccg
     2641 cgctgccccg cgcccttccc aacctcgccc ttcccaacct gggacattcc cgcatcaacc
     2701 tgggacgtct gcgtccgcgt tccaacctgc gacgcttcag gatcaacctg ggaagaactg
     2761 tgcgtgtcga atgcgcgttg gggtggtggg ggcggaatgg tctcgcctgt ctcgatcaca
     2821 tgaccacggc tgattacccg catctcgccg ggcacaaaac gcgaccaggg ttctccagac
     2881 aagggtcgtg tggaaatgat ggtcgacgtc cggttctcgt cgagcgagtt cgagaggtcg
     2941 agctcgagca agtccgactc gagcttctca gaggttggtg gtggcaggcg ccgaagatgg
     3001 tgta
//
LOCUS       DASRZF010000051         2500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3821080, whole genome shotgun sequence.
ACCESSION   DASRZF010000051 DASRZF010000000
VERSION     DASRZF010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3821080"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="VFG22_02300"
     CDS             <1..720
                     /locus_tag="VFG22_02300"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HET6415100.1"
                     /translation="LDSQKRVTPTNRELVAQGVGNTISGLLGGLPLTQVIVRSSANIQ
                     SGAKTKASGIIHGVMLLLAVLFLASLLNLVPLAVLASILLVVGYKLAKPALFVSMYRQ
                     GAAQLVPFVVTIAGILLTDLLSGVMLGLGIGVMGVLYRSYSNSHWVHVEEHDEAASGH
                     HVHVRLAEHVPFLSRGAILSRLAAIQNGSHVTIDLSNTVVLDHDVLEILEDFRQSAES
                     RDLEIRTIPPAREIDVIPNAA"
     gene            775..1401
                     /locus_tag="VFG22_02305"
     CDS             775..1401
                     /locus_tag="VFG22_02305"
                     /inference="COORDINATES: protein motif:HMM:NF011765.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HET6415101.1"
                     /translation="MHTHTLDTRDRLTHADALQILKDGNARFCANLKANRNLLQQVNE
                     TRDGQWPFAVILSCIDSRTSAELIFDQGLGDVFSVRIAGNFLNDDILGSMEFACHVAG
                     AKLVVVLGHSECGAIKGACDHVELGHLTGMLHKIQPAVDAVKNPADPSKRCSANASFV
                     DAVARENVLRSVSGIAERSEVLRGMRASGAIEIVGGIYDIASGAVEFF"
     gene            complement(1687..>2500)
                     /locus_tag="VFG22_02310"
     CDS             complement(1687..>2500)
                     /locus_tag="VFG22_02310"
                     /inference="COORDINATES: protein motif:HMM:NF015971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415102.1"
                     /translation="QVTPQFGEQADAINLAQATRAIHWLASSNYECSFAPDSDLSERV
                     LFPAGPIESHGIEDARFVRFTCDDGTVTYFAPYTAFDGHQILPQLIETADFVSFHVST
                     LTGAAAKNKGIAFFPRMIDGRYAALSRLDNENNFLMWSDDVRSWHHGEVIQRPVRPWE
                     ITQIGNCGSPLETEAGWLVITHGVGALRQYAIGAVLLDKEDPARVIGHLAEPLLEPTD
                     LERVGYVPNVVYSCGSMISGGLLVLPYGFSDVGAQIATVRLDDLLGALTGAR"
BASE COUNT          473 a          795 c          783 g          449 t
ORIGIN      
        1 ctcgactccc agaaacgcgt cactccgacc aatcgcgaac tggtggcgca gggcgtgggc
       61 aacacgatct cggggctgct cggagggctg ccgctcaccc aggtcatcgt gcgcagttcg
      121 gcgaacatcc agtctggcgc aaaaaccaaa gcatcgggca tcatccacgg cgtgatgctg
      181 ctgctcgccg tgctgttcct ggcgagcctt ttgaacctcg ttccgctcgc cgtgctcgcc
      241 agcatcctgc tggtagtcgg atacaagctg gcgaagccag cgttgttcgt gagcatgtac
      301 cgccagggcg cggcacagct cgtgcctttc gtggtcacga tcgccgggat cctgctcacc
      361 gatctactga gcggcgtcat gctcggcctg gggatcggcg tgatgggcgt cctctaccgc
      421 agctattcga attcgcactg ggtgcacgtc gaggagcacg acgaagcggc atccggacac
      481 cacgtgcacg tgcgactggc cgagcacgta ccgtttttga gccgcggtgc gatcctgagc
      541 aggctcgccg cgatccagaa cggttcgcat gtgacgatcg acctcagcaa caccgtagtg
      601 ctcgaccacg atgtgctcga gatcctcgag gacttcaggc agtcggccga gagtcgagat
      661 ctcgagattc ggaccattcc gcctgcccgc gagatcgacg tgattccgaa cgcggcttga
      721 tcgcgctggc gcccgcccta ccccccttga tttcgaatcc gaggagttcc gtacatgcac
      781 actcacactc tcgatacgcg cgaccgccta acacacgctg atgcgctgca aatcctgaag
      841 gacggaaacg ctcgcttctg cgccaacctg aaggcgaacc gcaacctcct tcagcaggtg
      901 aacgagacgc gcgacggtca gtggcccttt gccgtgatcc tgagctgcat cgactcgaga
      961 acctcggcag agctgatctt cgatcagggt ctgggcgacg tcttcagcgt gcgcatcgca
     1021 gggaacttcc tgaacgacga catcctcggc agcatggagt tcgcctgtca tgtggcgggt
     1081 gccaagctcg tagtcgtact cggacactcg gagtgcggcg ccatcaaggg agcgtgcgat
     1141 cacgtcgaac tcggccacct gaccggcatg ctccacaaga tccagccagc ggtcgatgcc
     1201 gtgaagaacc cggccgaccc cagcaagcgc tgttctgcga atgcgtcttt cgtcgacgcg
     1261 gtcgcgcgag agaacgtgct ccgcagcgtc tcgggcatcg cggagcgaag tgaagtgctg
     1321 cgcggcatgc gcgcctcggg cgcgatcgaa atcgtcggcg gcatctacga catcgcgagc
     1381 ggagcggtcg agttcttcta gcaagcccgc aaagagcatg gggaattcag actgcgcttt
     1441 gactcgtgtc gacgtccgag tacgggtctt atgccaagcc cctacgggga gcccgcaccg
     1501 gggctcgctt caccgcgcga cccagcgcac ccgcgtgcag acttcccagc tgcccattgt
     1561 gaagtcggca gacctcacga cacggcgcat cgcgctggtg gaggcccgcg ccgaggcttc
     1621 tccgattcag ctttcccctg cacgctccgg gagccgtcct cgagtttgaa ccgacgcggc
     1681 tgcaggtcaa cgcgccccag tgagtgctcc cagaagatca tcgagtcgca cggttgcgat
     1741 ttgcgcgccg acgtcggaga acccatacgg caggaccagc aacccaccgg agatcatgct
     1801 gccgcacgag tacacgacgt tggggacgta accaacgcgc tcgaggtcgg tcggctccag
     1861 cagcggctcg gcgaggtgac cgatgacgcg agcagggtcc tccttgtcga gcaagaccgc
     1921 cccgatcgcg tattggcgca gcgccccgac accgtgggtg atcacgagcc accccgcttc
     1981 ggtctcgagg ggcgatccgc agttaccgat ctgcgtgatc tcccaggggc gcacgggcct
     2041 ctggatcacc tcgccatgat gccaagagcg aacgtcatca gaccacatga ggaagttgtt
     2101 ctcgttgtcc agtcgggaaa gcgccgcata gcgcccatca atcatacggg gaaagaacgc
     2161 gatgcccttg ttcttggcag cggcaccggt gagcgtcgag acgtgaaacg acacgaagtc
     2221 ggcggtttcg atcagttggg gcagaatctg gtgcccgtcg aacgccgtgt acggagcgaa
     2281 gtacgtcacg gttccgtcgt cacaggtgaa gcggacgaag cgcgcgtctt cgatcccgtg
     2341 gctctcgatc ggtcctgccg gaaacaagac tcgttcggat aggtccgagt caggcgcgaa
     2401 ggagcactcg tagttggagg aagcgagcca gtggatcgcg cgtgtcgctt gcgcgaggtt
     2461 tatggcgtcg gcttgctctc cgaattgcgg agtgacctga
//
LOCUS       DASRZF010000052        16592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4165342, whole genome shotgun sequence.
ACCESSION   DASRZF010000052 DASRZF010000000
VERSION     DASRZF010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 16592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16592
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4165342"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..973)
                     /locus_tag="VFG22_02315"
     CDS             complement(194..973)
                     /locus_tag="VFG22_02315"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6415103.1"
                     /translation="MVLALDLHVEAFTRDGVDILRDIRWKIEAGEHWVVLGPNGSGKS
                     TLLSIVAGYEWPSQGRVVVLGETYGQCDMRALKERIGWVSSALFDWLPPQQSARQVAA
                     TGIHATIGNWHELSAHDLIRADDALRSIGAYAFRNKRYGILSQGEKQRVMIARALVTE
                     PSLLILDEPCAGLDPGARELLIDDLDTLCAQKKGPTLILVSHHVEEIPTCATHGLLLK
                     EGHTLAAGPLSEVLRDDTISELYEHTCQVGAANGRYWLRLR"
     gene            1059..2570
                     /locus_tag="VFG22_02320"
     CDS             1059..2570
                     /locus_tag="VFG22_02320"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HET6415104.1"
                     /translation="MPRHLLWPFVCIVLGTSAAKAQEPCSIAITGTVPEGPETHFYVE
                     FEVPEGTVEIEVQHDDLSSANILDWGLNDPNGFRGWGGGNSEPAVVGVEAASRSYVPG
                     PIPAGTWAVVVGKARISETPAEYELCITLRTEATPRPQTRAPYQDPGVLVDEARWYAG
                     DFHVHSQQSGDADPTIDETLRYADGVGLDFIMLSEHNTNSGLTLYANSQSDFPELLIV
                     PGVEWTTYSGHANALGATVWVDHKIGVNALTAAAAIQQYHDQGALFSINHPTVPGVPF
                     CIGCPWEIEVDPKTIDGVEVQGGIWEGIGYWEEVCAAGSHAAALGGSDDHRAGQADGV
                     LDSPIGTPTTMVFAENLSVEAILEGVRSGRTVVKVNGIDGPMLETDLSGERMGDTVFA
                     DSATLSAVVRGAAGQTLQVIKNGSTIESVSITSDPFTHETSVEAPIEGEDRYRHQVTN
                     GVSPQTVGSYVWLSAGTSSGCDCRIANESDRDTAVLILSLAFVVVLLRRRRSC"
     gene            complement(2574..3209)
                     /locus_tag="VFG22_02325"
     CDS             complement(2574..3209)
                     /locus_tag="VFG22_02325"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016471 - vacuolar proton-transporting
                     V-type ATPase complex [Evidence IEA];
                     GO_function: GO:0008553 - P-type proton-exporting
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HET6415105.1"
                     /translation="MSVRSVPPTRSGLVRANAQLGRVRKGRELLNRKREALITELFKS
                     ARPALNAREKVEERAAEAYRTLLRALAARGQASLETYAWPGREVNVELEQRQIWGIAV
                     PMVVSHSPIQRTLTARETAAPSVGPAAAQTASEFERLIELVLEALPKEQLVRRLGDAL
                     AKATRQVNTLQLRVEPTLTAELGALRSMLEERERDERSRLRHVLRRRKETL"
     gene            complement(3206..4615)
                     /locus_tag="VFG22_02330"
     CDS             complement(3206..4615)
                     /locus_tag="VFG22_02330"
                     /inference="COORDINATES: protein motif:HMM:NF003235.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HET6415106.1"
                     /translation="MKRLEAPIARTYAGAASAAGPLLFVRSTRRARLGEWVRIEGEGQ
                     ASRRGQVIDAGTEITVIQVFEDTLGLKPASASITLSGETATTVVGKDLLGRALSGTGA
                     PLDGLPASIGEARLPIWGAPINPVRRQHPSDFIETGVSAIDGMNTLVRGQKLPVFSAP
                     GLPGVELAADIVENARPARGEPFAVVFAAIGITERETQAFVSRFRESEVMERTVLFLN
                     QASDPTIERLLTPRVALTTAEHLAFTHGIHVLVVMADITHYAEALREVAAAREEIPGR
                     RGYPGYMYTDLASLFERAGLLKDGAGSVTQLPVVSLPDGDMTHPIPDLTGYITEGQIV
                     LSRELHQRGVYPPVDVLPSLSRLMNAGIGEGRTVPEHREWADQLYAVYARGREAKLMA
                     AIVGSEGLSPGDRRALEFVDAFERELIDQRRARRSLAETIKVGWSLLETLPRDELTRI
                     SDAAWNARTGSDRKAEAKP"
     gene            complement(4612..6348)
                     /locus_tag="VFG22_02335"
     CDS             complement(4612..6348)
                     /locus_tag="VFG22_02335"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF003220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HET6415107.1"
                     /translation="MTEARITRVAGAIAEAQPLGDAALYELVEVGNERLLGEVIRLKG
                     DVATIQVFEETTGLGLEEPVRRTGSSLMTELGPGLLGSILDGVGRPLTGLADRSGDFM
                     APGMRLPTLNPKALWSFSPVAKKGATVSGGDVLGTVEERPGMIHQVMVPPGVGGCIAS
                     IERGEFSVSDTVASLEDGTALRLSQSWPIRERRPFGRRLPGDRPFITGQRVFDLLFPV
                     VEGGSVAVPGGFGTGKTVIEQSLAKYAKADVMVYIGCGERGNEMAEVLHDFGRLTDPK
                     TGRPIMDRTILIVNTSNMPVAARDASVYLGMTIAEYYRDMGFHVAILADSLSRWAEAL
                     REIAARLQEMPGEEGYPTYLANRMSRLYERAGRVESEGTPARTGAVTFITAISPPGGD
                     FAEPVTQASLRVVGALWALDARLAHQRQFPAVDWDTSYSLYADGTRDWFSEHGGSDWL
                     ELRIETIRLLQRDRELREIAGLVGVDALEDEDRLVLEGARIAREFLIGQNAFHPHDAF
                     SSVSKTYHLAKLLWTFMQAGEAALKEGVSFESLDLAAVRVAFGSVKTAAPDELEREIE
                     RAGQTISEMRES"
     gene            complement(6345..6674)
                     /locus_tag="VFG22_02340"
     CDS             complement(6345..6674)
                     /locus_tag="VFG22_02340"
                     /inference="COORDINATES: protein motif:HMM:NF014092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HET6415108.1"
                     /translation="MKGERLVVVASPGLSAGFALAGVPVFQASDGAEAARQIQHATDE
                     AKAGVIIIDEPLYRELTEDLRRTLERSTLPLVIPVPGPDWTTESSAHEYIVEILRRAI
                     GYRVRLQ"
     gene            complement(6671..7687)
                     /locus_tag="VFG22_02345"
     CDS             complement(6671..7687)
                     /locus_tag="VFG22_02345"
                     /inference="COORDINATES: protein motif:HMM:NF014094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HET6415109.1"
                     /translation="MARYFGDINTRARGLRTHLLSSGDLERLARATSLTALQRELGAL
                     GYAGADGPATPGSLEQSVRRWAAGQLAVLERWCTDQRRAALSVIFEDEDRRSIQSLLR
                     GAARGTATETRMTALLPTRGLPERALLTLAKQPTPADVVRMLVLWNHPFGSALVRAAS
                     GSHPSLFEMEIELHRVFAQRASASAKEGGRQLVEYVQGVVDLMNAWSALLHFEERDPS
                     IVDLTFVEGGRWITRDLFQDLMGLDTRGDVERQLASSLRSSALGAIFRGKTEDLAELE
                     GAALRAQIAWQNRAVRLDPSGAAPVLGFAIELRAQVLNLMRIVWGVALRAPAALIQAE
                     MVMA"
     gene            complement(7690..8271)
                     /locus_tag="VFG22_02350"
     CDS             complement(7690..8271)
                     /locus_tag="VFG22_02350"
                     /inference="COORDINATES: protein motif:HMM:NF014093.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HET6415110.1"
                     /translation="MRASDLDSELRRSAERTVASVRAEALQEAARLASKADRAIAERR
                     KEVIENKEAEYLAEGRVAIAAARHAAMRAVLLARTRLVERVLGGARALLTEAAQSQSY
                     LSTLGEDTAAALQFIDDSGAVVRCSPSLESNMRELLRERTNLNIEADAELGTGFVVIG
                     DGGSVRVDGRLESRLDRLSSALAIEIHSRLEKT"
     gene            complement(8279..8506)
                     /locus_tag="VFG22_02355"
     CDS             complement(8279..8506)
                     /locus_tag="VFG22_02355"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415111.1"
                     /translation="MEMDTIWIYLSAALAVGIPAMATGWAQSRIGPAAAAALAEKPEL
                     TVTVVLLIAIPETVAILGFVISVLILMQGGG"
     gene            complement(8508..10388)
                     /locus_tag="VFG22_02360"
     CDS             complement(8508..10388)
                     /locus_tag="VFG22_02360"
                     /inference="COORDINATES: protein motif:HMM:NF013647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HET6415112.1"
                     /translation="MSRIRLLGPRNQLQAVLARVQDLGLVHLVEPPETEALSRLGLTV
                     EQQRHVKRVRKVGADVDRAIELLGERPSSPKAVKDLSLSQAGLIAGRCRRQLESLTER
                     RQLLEEERALILKFRPFFSTFRRIADSGLDLDGIRVFYLVLREDGEQELRRMRVALRE
                     AIGDGFELLSQPSESGESAMLLAVPSANAKKAELLLSETGVQALSLPEQFEEGSVVAT
                     VQRMQDRLLELPAELAAVEEERRQIAKERGRDLARIQAGVHDHLAKIEAMGQALVTPR
                     AFILEGWVPSAALPELENRLEKSFGNQIETETVSSAEWAGDTVPVVLHNPRLFRPFES
                     ITRMMPLPKYGTVDPTPFVAIFFPMFFGVVLGDVGYGAMLGIISLILRIRSPAGGKLR
                     SVSEIGLACALFAIIFGFLYGELFGDLGHRIGLHPIIFNREEAFFPFLGFAVALGIVH
                     IVVGLLLGAVKLFRGDKRRAVGKGIAALMVVLIALALMAAFEMLPQRFFTPLVISVLV
                     AFPLLIIAEGVLAPVELISTLGNVLSYARIMAVGTASVMMAIVANRMAGAFGSVVVGA
                     LFALLFHLINFVLGVFSPTIHMLRLHYVEFFGKFYSPGGSQYRPFRHWSARDSAFAER
                     SS"
     gene            complement(10397..10774)
                     /locus_tag="VFG22_02365"
     CDS             complement(10397..10774)
                     /locus_tag="VFG22_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415113.1"
                     /translation="MAENRKSKSERAAADAKTKLRRLLETERELEEILKGAKREAKEL
                     VESARAAADERVRLLETQLASEDAELRERVAHDRDETIASIREQAREETERLDGLDPA
                     KLDELSRHVVDLLIGGRDSGGRH"
     gene            complement(10826..12004)
                     /locus_tag="VFG22_02370"
     CDS             complement(10826..12004)
                     /locus_tag="VFG22_02370"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6415114.1"
                     /translation="MSPREQPLTKPRNPPTLLTREFLLLLASVSLFGFSWSFYLILPK
                     FFATELGMDAAAIGRAVAVQGLTAVAATPLVGWLVDRFGRRPWLIAGNFMLALTGLLY
                     VFVDHEGPLLYLAQMCWGMGMVMGFNAAGTMTADIAPSDRMAQALGLFGAANLGMNAV
                     SPTIGEILADSKGWQYVFVASAMAGAIATLLSTRLKETPRHAAHEHESRRPILGGSLL
                     RVYGATFVMTAAFTALFTLHQPFALQLGVTEVRSFFIGFAIVALTVRLGGGRLIDRFG
                     VLRASVIAFCLYSAVPPMLGVLGPDHLFAIGAMMGLGHGIAYPALTALGIQRADVSAR
                     GMVVSIIHGAFNGGHAFFAYALGLIAAAWSYDLAFWIAGMVTLTGALLLGLGRPSKAV
                     "
     gene            12183..12668
                     /locus_tag="VFG22_02375"
     CDS             12183..12668
                     /locus_tag="VFG22_02375"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET6415115.1"
                     /translation="MSSRPVTIAPDVCVSEAFRVARSFGVQHLVVTEGRLPVGVICVR
                     CDLADALPTRPIDEYMRQPITIGAYETAERASLQMCEKGLGALIVRGGDRGWGIATRG
                     DLLRAGFRAGDESALFFCAACGTHMHVHPVPGCEWVAFCVDCWDRAGPPRIGDDLGES
                     D"
     gene            12668..13531
                     /locus_tag="VFG22_02380"
     CDS             12668..13531
                     /locus_tag="VFG22_02380"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HET6415116.1"
                     /translation="MRAFLTGATGTVGREVCRGLLAAGAEVRVGTRASSHPGGTLPNV
                     STVIPLDFEDPGINRAVFEGVDVFFFMTPLIDDQVAASIRVLDAAIEAGVRHVVRLSS
                     RSAGWDQVSVLRLWHREIEDAVRSSGASWTILRPCSFFQNFIKYQSEAVRNMSSVVLP
                     LGDGLIPFIDVVDIGEAAVKCLLHSEDHAEKTYVLTGGRAYGAKGVAAEIGRVIAKPV
                     TYIDVEPEQAREMMSQSGVPPWLVEAGLAVFAHAKAGGEAAVDPALTEIIGRPATSLS
                     EFVERNRRAWL"
     gene            13594..15063
                     /locus_tag="VFG22_02385"
     CDS             13594..15063
                     /locus_tag="VFG22_02385"
                     /inference="COORDINATES: protein motif:HMM:NF018471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HET6415117.1"
                     /translation="MAIRPDDRTYALSSWLFARSLGAVLFIAFVSLGAQAKGLFGAHG
                     VAPISALVESAKAAGHGFWQHPSALWWSNGDAMITSVCIVGASSSLALLVGLVPRLAL
                     LLCWFAYLSFVSLGWPFMSFQWDTLLLEVSFTSIFFVPWGLRDRRGDHAEPHPVARWA
                     LWWLLFRLVFRSAYVKLASGDPSWANLSALSYHYWTQPLPTTLAWYANLLPAWVQRVS
                     CALMFLVELGAPVLLFVPRPWARRTAAGSIAALMVLIALTGNYGFFNLLTIVLCLPLL
                     DDRLLLRLTPARLTPSSDSSRRIGSNPWSGLAPAMVIAVSAAVFFTGTLTGRPPAWLG
                     LVYRTSTFNNYGLFSVMTTERREINIEGSSDGETWKPYAFRYKPGPLDRAPVWVAPHQ
                     PRLDWQMWFAALGDYRRNDWLANLMTRLLQGERTVLALMEKNPFEDEPPKQVRAVIYR
                     YEFTDPEERHATGNWWKRSDRFLYAPILGVEVEPTSTVE"
     gene            complement(15047..16486)
                     /locus_tag="VFG22_02390"
     CDS             complement(15047..16486)
                     /locus_tag="VFG22_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415118.1"
                     /translation="MKHNLLTASLSLGLLIPAFASANPEPTAYDTRSIAMGLTGVTFL
                     DRPAALALNPANLEGIPKLGFTVNFTGLLVNQTAPVQGPNTSVSSGLGFGPLPSGFIA
                     GRIAPRVVFAGGIYIETGYGSSFSGVKCLDGAAVTPNPSSQPDPRPDNWVPATETSDP
                     QCLNPQAEDLDVTFFVGEFAAGFSFRVHEKFNLGLALRLPFSKQTADLYQNVGAAFGN
                     VGYGRVKNDLGGVGIPALRAGFTIKPHRKVRISAMYRMATKIKLTGETETELAAGAEP
                     LKLQSEADWFIPHAIAFGVSYQANSHLLLAFEGRLQFHGADKSGNKNQTVIASSDGTV
                     LQTIVVPFGWENTWSAKVGFEYRFTKDVLALRLGANIAKSATSGEFAQYFTPPPGLSG
                     NVAGGLGFYWDDRNDPSIKDRYMLDIGTAFAFTTGTIGNEYIGTTAQIPGTDTDVILC
                     SDDQVVRTGCPGKYKVFTYWASLSFTLQY"
BASE COUNT         3082 a         5247 c         5125 g         3138 t
ORIGIN      
        1 aaacctcggc gcgacgatca tacacgctcc cgaggaggcg gtctgggcac ccggctacta
       61 ctccatcctc ttcgaagacc cggatggtat ccgtctagag gtcaactacg tcccggggaa
      121 gggcctactc gagtaggctg cgaggagcgg ggccaggcac cgtttcgtag ctcgagcggg
      181 tttgctgcca ccgctaacgg aggcgcagcc agtagcgccc attggcggcg ccgacttggc
      241 acgtgtgttc gtagagctcg ctgatggtat cgtctcgcag gacctcgctg aggggacctg
      301 ccgctagggt atggccttct ttgagcagga gcccatgggt ggcgcaggtc gggatctctt
      361 cgacgtggtg gcttactaga atcagcgtgg ggcccttctt ctgggcgcag agcgtgtcta
      421 ggtcgtcgat cagcagttct cgcgccccgg ggtcgaggcc ggcgcaaggt tcgtcgagaa
      481 tcagcaagct cggctcggtc accagggctc gggcgatcat cacccgttgc ttctcgcctt
      541 gggataagat cccgtagcgt ttgtttcgga aggcgtatgc tccgatactg cgcaaggcgt
      601 cgtcggctcg aatgaggtcg tgggcgctga gctcgtgcca gtttccgatc gtcgcgtgga
      661 tgcccgtcgc agcgacctga cgagcgcttt gctgcggagg gagccagtcg aacagcgccg
      721 agctaaccca gccgattctc tctttgaggg cgcgcatgtc acattgcccg taggtttctc
      781 ccagaacgac gacgcgcccc tggctcggcc attcgtagcc ggcaacgatc gagagcaagg
      841 tgctcttccc ggaaccgttc ggaccgagga ccacccagtg ctcacccgct tcgatcttcc
      901 agcgaatgtc cctcaggatg tcgacgccgt cgcgagtgaa ggcttctacg tgcaaatcga
      961 gagcgagaac cacccaagaa gcctagcatc gtctgaggat aggccgcctc ctccttaggg
     1021 cttgatgtag ccgcagatgt gggttccaat gcgtcgatat gccgcgacat ctgctttggc
     1081 cgttcgtttg catcgttctc ggaaccagcg ctgccaaggc gcaggagccc tgctcgatcg
     1141 cgatcaccgg caccgttccg gaggggcccg aaacgcactt ctacgtcgag ttcgaggttc
     1201 ctgaagggac cgtcgagatc gaagtgcagc acgacgacct ttcgtcggcg aacattctcg
     1261 attggggcct caatgatccg aacgggttcc gcgggtgggg agggggaaac agcgaacccg
     1321 ccgtcgtagg cgtggaagcc gcctctcgca gttacgttcc tggtccgatt ccggcgggca
     1381 cctgggcggt ggtcgttggc aaagccagaa tctcggaaac acccgccgaa tacgagcttt
     1441 gcatcactct gcgaacggaa gcgacgccgc ggcctcaaac acgcgcgccg taccaagacc
     1501 ccggcgtcct cgtcgacgaa gcccgctggt atgcgggcga cttccacgtc cactcgcaac
     1561 agagcggaga tgccgacccg accatcgacg agaccctccg atatgcagac ggtgtggggc
     1621 tcgacttcat catgctgtcc gagcacaaca ccaacagcgg actgacgctc tacgcgaatt
     1681 cacagtctga ttttccggag ctgctgatcg ttccaggcgt cgagtggacg acctactcgg
     1741 ggcacgccaa cgcgctcggt gcgaccgtat gggtcgatca caagatcggc gtgaatgctc
     1801 tgaccgcggc cgccgccatc cagcagtatc atgatcaggg tgcgctcttc tcgatcaatc
     1861 atccgaccgt acccggcgtt cccttctgca ttggctgccc ctgggagatc gaggtcgatc
     1921 ccaagacgat cgacggcgtc gaagttcagg gcggaatctg ggaaggtatc ggttactggg
     1981 aagaggtatg cgccgcgggt tcccatgccg ccgcgctcgg tggcagcgac gatcacaggg
     2041 caggtcaggc cgacggtgtg ctcgacagcc cgatcggcac gccgaccacc atggtatttg
     2101 ccgaaaacct cagcgtcgag gccatcctcg agggtgttcg cagcggccgc acggtcgtca
     2161 aggtcaatgg gatcgatgga cccatgctcg agacggatct cagcggcgag cgcatgggtg
     2221 acacggtctt cgccgacagc gcgactctgt ctgcggtggt tcgaggcgct gcaggtcaga
     2281 cgctgcaagt catcaagaac ggctcgacga tcgagagcgt atcgattacc tcggacccat
     2341 tcacgcacga aacgagcgtc gaggctccga tcgaaggaga ggaccggtac cgtcaccagg
     2401 tgacgaacgg agtctccccc cagacagtcg ggagctatgt ctggctgagt gccgggacct
     2461 cgtccgggtg tgactgccgc atcgcgaatg agagcgaccg cgacacggct gtcctgatac
     2521 tgagcctcgc atttgtcgta gttctcttgc gccggcgcag gtcctgctga agctcagagc
     2581 gtctctttgc gccttcgcag cacgtgcctt agccgagaac gctcgtcgcg ctctcgctcc
     2641 tctagcatcg accgaagggc gcccaactcc gccgtcaatg tgggctccac gcgaagctgc
     2701 agggtattga cttgccgagt cgctttcgcc agggcgtcac cgagacgccg cacgagctgc
     2761 tccttgggca gcgcttcgag aacgagctcg atgagccgct cgaattcgct tgcggtctga
     2821 gccgctgcag ggcccacaga cggagccgcc gtttctcgtg cagttagcgt gcgctgaatt
     2881 gggctgtgcg acacgaccat gggtactgcg ataccccaaa tctgtcgttg ttcgagctcg
     2941 acgttcacct cgcgtccagg ccatgcgtag gtctccaacg aggcttgacc tcgcgccgcc
     3001 aacgctcgaa gcagcgttcg gtaggcctca gcagccctct cttctacttt ctcgcgtgcg
     3061 ttcaacgcgg gcctcgcgct cttgaagagc tcggtgatca aggcctccct cttgcgattc
     3121 aagagctcgc gacctttccg gacgcgaccg agctgagcat tggcgcgcac cagaccagat
     3181 cgcgttggcg gaacggaacg aacactcatg gcttcgcctc ggcctttcga tcgctgcccg
     3241 tccgagcatt ccacgcagca tcggaaattc gggtgagctc gtcgcgagga agcgtttcga
     3301 gtagcgacca gccgaccttg atcgtttccg cgagagatcg ccgagctcga cgttgatcga
     3361 tgagctcccg ttcgaacgcg tccacgaact cgagcgcgcg cctgtcaccg ggcgaaagac
     3421 cctcgcttcc gacgatggcc gccatcagct ttgcttccct gccccgcgcg tagacggcat
     3481 agagctgatc cgcccattcg cggtgttctg gcaccgtgcg accctcgccg atacctgcgt
     3541 tcatgagtcg tgacaaggat ggcaatacgt cgacgggggg atacacgccg cgctgatgga
     3601 gctcccggct caagacgatt tgtccttcgg tgatgtatcc ggtgagatcc ggaatcggat
     3661 gggtcatgtc gccgtcggga agggagacga ccgggagctg cgttaccgaa cccgcgccgt
     3721 ccttgaggag tcctgcgcgc tcgaacaagg acgccaggtc ggtgtacatg tagcctggat
     3781 agccgcggcg ccccggaatc tcttcgcgag ccgcagcgac ctcgcgcaac gcctccgcgt
     3841 agtgggtgat gtccgccatc acgacgagca cgtgaatgcc gtgcgtaaag gcgaggtgct
     3901 ccgcagtggt caaggcgacg cgcggcgtga ggagtcgttc gatcgtggga tcgcttgcct
     3961 ggttgagaaa cagaacagtg cgctccatga cctcgctctc gcgaaaccgc gagacgaacg
     4021 cttgagtctc ccgctcggtg atcccaatcg cggcgaatac cactgcgaat ggctctccgc
     4081 gagccgggcg cgcgttttcg acgatgtcgg ctgcaagctc gaccccagga agacctggag
     4141 ccgagaacac cgggagcttc tgtccgcgca cgagggtgtt cataccgtcg atcgccgaca
     4201 cgccggtttc gatgaagtcc gagggatgct gccttcggac cgggttgatc ggcgcgcccc
     4261 agattggaag gcgcgcctca ccgatcgaag ccggcaagcc atcgagaggc gcaccggtgc
     4321 cactgagcgc gcgtccgagg aggtccttcc cgacgaccgt cgtcgcggtc tcgcccgaca
     4381 aggttatcga agcagacgca ggcttgagcc cgagcgtatc ctcgaaaacc tgaatgaccg
     4441 tgatctcggt gccggcgtcg atcacctgcc cccgccggct cgcctgcccc tcgccctcga
     4501 tgcgaaccca ctcccccagc ctcgcgcgcc gcgtcgagcg gacgaaaagc agagggccgg
     4561 ccgcagaggc agcgcccgca tatgtccgcg cgatgggggc ttccagtcgc ttcatgattc
     4621 cctcatttcg gaaatcgtct gcccagctcg ttcgatttct cgttcgagct cgtcgggtgc
     4681 ggcggtcttc acggatccaa acgcaacacg aacggcagcg aggtcgaggc tttcaaagga
     4741 tacgccttcc ttcagcgccg cctctccagc ttgcatgaat gtccacagca gcttggcgag
     4801 atggtaggtc ttcgaaacgc tcgaaaaagc gtcgtgcggg tgaaatgcgt tctgcccaat
     4861 caagaactct cgcgcgattc gcgcgccctc gagcaccaga cggtcttcgt cttcaagcgc
     4921 atcgacgccg acgagtccag caatttcgcg aagctctcga tcccgctgca acaatcgaat
     4981 cgtttcgatc cgaagctcca accaatccga cccaccgtgc tcggagaacc agtccctcgt
     5041 gccgtcggca tacaagctgt agctcgtgtc ccagtcgacc gcagggaatt ggcgctggtg
     5101 cgccaggcgc gcgtcgagcg cccagagggc acctacgacg cgcaacgacg cttgcgtcac
     5161 cggttcggca aagtcgcccc caggaggaga aatcgcggtg atgaacgtga cagcgcccgt
     5221 gcgtgcggga gtgccctcgc tctcgacgcg cccggcgcgc tcgtacaagc ggctcatgcg
     5281 gttcgccagg tacgttggat atccctcctc gccgggcatc tcctgcagcc gcgcggcgat
     5341 ctcacggagc gcctcagccc atcgcgagag ggagtccgcg aggatcgcga cgtggaaacc
     5401 catgtcccgg tagtactccg cgatcgtcat cccgagatag accgacgcgt cacgggcggc
     5461 gaccggcatg ttcgaggtgt tcacgatcag gatggttcga tccatgattg gacgcccggt
     5521 tttcggatct gtcagacggc cgaaatcgtg caggacctcg gccatctcgt tgccgcgttc
     5581 gccacatccg atgtacacca tcacatcggc cttcgcgtac ttggcaagag actgctcgat
     5641 cacggttttc ccagttccga aaccgccggg tacggcgacc gaaccccctt cgacgaccgg
     5701 gaacagcagg tcgaagaccc gttggcctgt gatgaagggc ctgtcaccgg gcagtcggcg
     5761 tccgaagggt cgccgttcac gaatcggcca agactgcgag agacggagcg cagtcccatc
     5821 ttccaagctc gccaccgtgt ccgagacact gaactctccc cgctcgatgc tggcgatgca
     5881 ccctccgacg ccaggcggca ccatgacttg gtggatcatt cctgggcgtt cctcgacggt
     5941 gcccaatacg tctccgcccg acaccgttgc acccttcttc gctacgggtg aaaacgacca
     6001 gagcgccttg gggttcaggg tgggcagtct catgcccggc gccatgaaat caccggaacg
     6061 atctgccagg cctgtgagcg gtcgacccac gccgtctagt atcgaaccga gtagcccagg
     6121 accgagctca gtcatcaagg agctgcccgt cctccgaact ggttcctcga gcccgaggcc
     6181 ggtcgtctct tcgaagactt ggatggtggc gacatccccc ttgagacgaa tgacttcgcc
     6241 gagcaggcgc tcgttcccca cctccaccag ctcgtaaagg gccgcatcgc ccaacggctg
     6301 tgcctcggcg atggctccgg cgactcgagt gattcgcgcc tccgtcattg gagcctcacg
     6361 cgatagccga ttgcgcgccg aaggatctcg acgatgtact cgtgcgcgct ggattcggtc
     6421 gtccagtcgg gcccgggaac cgggatgacc aggggcaagg tgctacgctc gagtgttcgc
     6481 ctcaaatctt ccgtcagttc ccggtagagc ggctcatcga tgatgatcac gcccgctttc
     6541 gcctcgtccg tggcatgctg gatttggcgc gcagcctctg caccgtccga cgcttggaac
     6601 acggggactc cggccagggc gaagccggcg ctcaggccag gacttgcgac cacgacgagt
     6661 cgttctcctt tcatgccatc accatctcgg cttggatgag ggcggcgggt gctcgaagcg
     6721 ccacgcccca gacgatgcgc atcaggttca gcacctgcgc gcgtagctcg attgcaaagc
     6781 ccagcaccgg cgccgctccg ctagggtcga gccggaccgc tcgattctgc catgctattt
     6841 gcgcccgaag cgcagcgcct tcgagctccg cgaggtcctc ggtcttgcct cgaaagatgg
     6901 ctccgagcgc cgagcttcga agagatgacg ccaactgccg ttctacgtcc ccgcgcgtat
     6961 cgaggcccat cagatcctga aacagatcgc gcgtgatcca gcgaccccct tctacgaacg
     7021 tgaggtcgac gatcgaaggg tctctctcct cgaagtgcag caacgccgac caggcgttca
     7081 tcaagtccac gaccccctga acgtactcga cgagttgcct tcctccttcc ttagccgagg
     7141 ccgacgccct ctgggcgaat acacggtgga gctcgatctc catctcgaag agcgagggat
     7201 gcgatccgct cgcggcacgc accaaagcgg aaccgaacgg atggttccaa aggaccagca
     7261 tgcgaaccac atctgcggga gttggctgtt tcgccagcgt caacagcgct ctttcgggaa
     7321 ggccacgcgt aggcagcagc gccgtcatgc gagtttcggt cgcggtgccc cgcgctgccc
     7381 cgcgaaggag agattggatc gacctgcgat cctcgtcctc gaagatcacc gaaagagcgg
     7441 cgcgtcgctg atcggtgcac cagcgctcga gcactgccag ctggccggcg gcccaccgac
     7501 gaaccgactg ctcgagactt cccggtgtcg ctggtccgtc agccccggcg taacccaggg
     7561 cgcccagctc tcgttggagc gcagtgaggc tggtggcccg cgcgagccgt tccaagtcgc
     7621 cgctcgaaag caaatgcgtc cgaagacctc gggctctggt gttgatgtcg ccgaagtagc
     7681 gggccatggc taggttttct ccaagcgcga gtggatttcg atggcaagcg cagatgagag
     7741 ccggtcgagc cgagactcga ggcgaccgtc gacccgaacg gacccgccat cgccgatgac
     7801 cacgaatccg gtgccaagct ctgcatccgc ttcgatgttg aggttggttc gttcgcgcag
     7861 cagctcacgc atgttggact cgaggctcgg tgagcatcgc acgaccgctc cgctgtcatc
     7921 gatgaactgc agagcagctg cagtgtcctc accgagggtc gatagatagg actggctctg
     7981 cgccgcttcc gtcagcaagg cccgggcacc cccgaggaca cgttcgacga ggcgtgtccg
     8041 cgcgagaaga acggcgcgca tcgcggcgtg ccgggcggct gcgatcgcga cgcggccctc
     8101 cgcgaggtac tccgcctctt tgttttcaat gacctcttta cgcctctcag caatcgcgcg
     8161 atcggccttg gacgcaagcc gagccgcttc ttgaagcgcc tccgcacgaa cggacgctac
     8221 cgtcctttca gcgctgcgac gcaactcgct gtcgaggtcc gatgcccgca tttgtccctc
     8281 agcctccccc ttgcatgaga atcaagaccg agatcacgaa gcccagaatc gccaccgttt
     8341 ccgggatggc gatcaagagc acgactgtga ccgtgagctc aggcttctcg gcgagtgctg
     8401 ccgccgccgc cggcccgatc cgcgattgcg cccatccggt ggccatcgcg ggaataccga
     8461 ccgcgagagc ggcgctcaag tagatccaaa tcgtatccat ttccatatca gctgctcctc
     8521 tccgcaaatg cgctgtcgcg cgcggaccaa tgtcgaaacg gccggtactg cgaaccgccc
     8581 gggctgtaga actttccgaa gaactccacg tagtggaggc gcagcatgtg aatcgtgggg
     8641 ctgaagacgc cgagcacgaa gttgatcaag tgaaagagca gggcgaacag cgcccctacg
     8701 acgacgctgc cgaacgcacc cgccatccga ttcgcgacga tggccatcat gacggaggcg
     8761 gttcccacgg ccatgatacg ggcgtatgaa agcacgttgc ccaaggtgga gatcaactcc
     8821 acgggcgcca agacaccttc cgcgatgatc aagagcggaa acgcaaccag caccgagatg
     8881 accaggggcg tgaagaagcg ttgaggcagc atctcgaatg ctgccatgag tgcgagcgcg
     8941 atcaagacca ccatcagcgc ggctatgccc ttgccgacgg cccgccgttt gtcgccacgg
     9001 aacaacttca cggctccgag caagaggccg acgacgatgt gaacgattcc gagcgcgacg
     9061 gcgaatccga gaaacggaaa gaacgcttct tcgcgattga agatgatcgg gtggaggccg
     9121 atgcgatgac ccaagtcgcc gaatagctcc ccgtagagga atccgaatat gatcgcaaac
     9181 agcgcgcagg cgaggccgat ctcggacacc gagcgcagct tgccgccggc tggagatcgg
     9241 atgcgcagga tgagcgagat gatgccgagc atggcgccgt atccgacgtc accgagcacg
     9301 acgccgaaga acataggaaa gaagatcgcg acaaatgggg ttggatccac cgtcccgtac
     9361 ttaggaagag gcatcattcg cgtaatgctc tcgaatggac ggaacaatcg gggattatgc
     9421 agaaccacgg ggaccgtatc ccccgcccac tcggcggacg acacggtttc ggtttcgatc
     9481 tgattgccga acgacttttc gagtcgattt tcgagctcgg gcagcgccgc gctcgggacc
     9541 cagccttcga gaatgaacgc ccgcggcgtc actagcgctt gacccatggc ctcgatcttc
     9601 gcgagatgat cgtgaacccc tgcctgaatg cgcgcgaggt cacggccccg ctctttcgcg
     9661 atttgcctcc gctcttcctc taccgctgcc agctcggctg gaagctccaa gaggcggtct
     9721 tgcatccgtt ggacggtcgc caccacactc ccttcttcga actgttcggg gagtgagagc
     9781 gcctgaaccc ccgtttcact cagcagaagc tccgccttct tggcgttggc ggaggggacg
     9841 gcgagcagca tcgccgattc cccggactcc gacggctggc tcaggagctc gaacccatcc
     9901 ccgatggcct cgcgcagggc caccctcatg cgtcgaagct cttgctcgcc atcctcgcgg
     9961 agcacgagat agaagacgcg aatcccgtcg aggtctagac ccgaatcggc gatcctacga
    10021 aacgtcgaga agaaaggacg aaacttgagg atgagcgcgc gctcttcctc gagaagctgg
    10081 cgccgttcgg tcaagctctc gagctgtctt cgacagcgac cggcgatcaa tcctgcctgg
    10141 gatagcgaga ggtccttgac cgccttgggt gagctgggtc tctcgccgag cagctcgatc
    10201 gctcggtcga cgtctgcccc taccttgcgc actctcttga cgtgacgctg ctgttccacc
    10261 gtcagcccga ggcgactgag cgcttcggtt tcggggggct caacgaggtg cacgagacca
    10321 agatcctgga cgcgcgccaa gacggcctgg agttggtttc tcggaccgag gagtcggatg
    10381 cgggacatgt ccaagatcaa tggcggcctc ccgaatctcg tccaccgatc agaaggtcga
    10441 cgacgtgtcg agatagctcg tcgagcttgg ccggatcgag cccgtccagg cgctcggttt
    10501 cctctcttgc ctgctctcga atcgaggcga tggtctcgtc ccggtcgtgc gcgacgcgct
    10561 ctcgaagctc tgcgtcttcg gatgcaagtt gggtctcgag caaacgtacg cgctcatcgg
    10621 ccgctgcgcg tgccgactcc accagctcct tggcctcgcg cttcgccccc ttgagaattt
    10681 cctcgagctc cctctcggtt tcgagaagac gtcggagctt cgtcttcgcg tcggcggccg
    10741 ctcgctcgct cttgctcttc cgattctcag ccatgaccaa gtactcagcg ggaaaatagc
    10801 agaaggcggc cgttcagggg tggcgttaaa ccgctttcga gggacgcccg agacccaaga
    10861 gcagcgcgcc ggtgagcgtc accatgccgg caatccaaaa agccaagtcg tagctccatg
    10921 cggcggctat gagaccgagc gcatacgcaa aaaatgcgtg accaccattg aaggcgccgt
    10981 gaatgatgga cacgaccatc ccgcgcgcag aaacgtcggc tcgctggata cctagggccg
    11041 tgagggctgg ataggcaatc ccgtgcccga gacccatcat ggcgccgatg gcaaacagat
    11101 ggtcagggcc gaggacaccg agcatcggcg gtaccgcgga gtacaggcaa aatgcgatga
    11161 ctgaggcccg aagcacgcca aaacggtcga tcaaacgtcc gccgccgagg cgtacagtga
    11221 gcgcgacgat tgcgaagccg atgaagaagg aacggacttc ggtcacgccg agctgaaggg
    11281 caaaaggttg gtgcagtgtg aacaacgcgg tgaacgcagc ggtcatcacg aatgtggcac
    11341 cgtaaacacg taacaacgaa cctcccagga tcgggcgtcg gctctcgtgc tcatgtgcag
    11401 cgtggcgtgg agtttccttc agacgggtgc tgagtagcgt tgcgattgcg cccgccattg
    11461 cactggcgac gaagacatat tgccaacctt tggagtccgc caggatttcg ccgatcgtgg
    11521 gtgacaccgc attcattccg aggttcgccg caccgaacag gccgagtgcc tgcgccatgc
    11581 ggtcgctcgg tgcgatgtct gcggtcatcg tgccggccgc gttgaacccc atgaccatcc
    11641 ccatgcccca gcacatctgt gcgaggtaga gtagggggcc ttcgtggtcg acgaacacgt
    11701 agagcaggcc cgtgagcgcc aacatgaagt tgccggcgat cagccacggc cggcgtccga
    11761 aacgatcgac caaccagcct actagcggtg tagccgccac cgcggtgagt ccttgcaccg
    11821 cgaccgccct tccgatcgcc gccgcgtcca tgccgagctc ggtcgcgaag aatttgggta
    11881 ggatcaggta gaacgaccaa gagaaaccaa agagcgaaac cgacgcgagc agaagcagga
    11941 actcgcgcgt cagaagtgtt ggcgggttcc gaggcttggt cagtggctgt tctctggggg
    12001 acactggaac cggtctttag cactccggcg ccgcctttgg cgcggagttc ggcgtccacc
    12061 gatggggcta ggaaaaccag cgcgatgctg gtaacgtccg atccacgatc agcgggctcg
    12121 cttgaaatat tcatttccga tcccgatcta gagaggggat gcgtatgcgt gttgcggacg
    12181 tcatgagtag tagacccgtc acgatcgctc cggacgtttg cgtttccgag gcctttcgag
    12241 tcgccaggag cttcggcgtt caacacctgg tcgtcacgga aggtcgcctg cccgtggggg
    12301 tcatttgcgt gcgctgcgac ctcgcggacg cgcttccgac gcgccccatc gacgagtaca
    12361 tgaggcagcc catcacgatc ggtgcttacg agacagcaga acgcgccagc cttcaaatgt
    12421 gcgaaaaagg ccttggtgcg ctgatcgtgc gtggtggtga ccgcggctgg ggaatcgcca
    12481 cgcgaggcga cctcttgcgg gctgggttca gagctggtga cgagtccgct ctcttcttct
    12541 gcgcagcatg tggcactcac atgcacgtgc accccgtgcc tggctgcgag tgggtcgcct
    12601 tctgcgtcga ctgctgggat cgagcagggc cacctcgaat cggtgacgat ctcggcgaat
    12661 ccgactagtg cgcgcatttc tcactggagc aaccggcacg gtcggtcgag aggtttgccg
    12721 tgggctcctc gctgcgggcg cagaggttcg cgtcggcacg cgcgcctcga gccaccccgg
    12781 cggcaccttg ccgaatgtga gcaccgtgat tccgctcgac ttcgaggatc ccgggatcaa
    12841 tcgcgcggtc ttcgagggtg tggacgtgtt cttcttcatg acgcccttaa tcgatgatca
    12901 agttgcggct tcgattcggg tgctcgatgc tgcgatcgag gcaggcgtac ggcacgttgt
    12961 gcgcttgtca tcgcgctctg ccggttggga ccaagtatcc gtgctgcgcc tatggcaccg
    13021 ggagatcgag gacgccgtcc gtagctcggg tgcatcgtgg acgatccttc gaccctgttc
    13081 gttcttccaa aacttcatca agtatcagtc ggaagcggtc cgcaatatgt cttcggtcgt
    13141 acttcccctg ggtgacggcc tcattccctt catcgacgtc gtggacatcg gagaagccgc
    13201 agtgaaatgc ctgctccatt ccgaggatca cgctgagaag acttacgtgc tcaccggtgg
    13261 tcgcgcttac ggcgccaagg gcgttgctgc cgaaatagga cgtgtcatcg ccaagccagt
    13321 cacttacatc gatgtcgagc cggagcaggc gcgcgagatg atgagccaga gtggagtgcc
    13381 cccgtggttg gtcgaggctg gcctcgccgt gttcgcgcac gccaaagctg gtggcgaggc
    13441 agccgtcgac ccggcgctga cggaaatcat cggccggccg gcaacgtcgc tctccgagtt
    13501 cgtagagcgc aaccgccgag cgtggctcta gccgcctcac gccccgtcgc cagcgctgac
    13561 actggcgctg acgatgtgat aatcagcgct tctgtggcca tccgacccga cgaccgaacc
    13621 tatgcgctct cttcctggct ttttgctcgc tctctgggcg cggtcctctt catcgccttc
    13681 gtctcgttgg gcgcccaagc aaaagggctc ttcggagcgc acggcgtcgc gcccatctcg
    13741 gcgctcgtag aatccgcaaa agcagccggg cacggtttct ggcagcatcc gagcgcgctc
    13801 tggtggtcga acggcgatgc gatgatcacg agcgtttgca tcgtcggcgc atcgtcctcg
    13861 ctcgctttgc tcgtcggact ggttccaaga ctcgcactcc tgctctgttg gttcgcctac
    13921 ctttcgttcg tgagcctcgg ctggccgttc atgtcgttcc agtgggacac gcttctgcta
    13981 gaggtgtcgt tcacttcgat ctttttcgtt ccatggggtc tccgggatcg acggggcgat
    14041 cacgcggagc cgcatccggt cgcacgctgg gccctttggt ggctgctctt ccggctggtg
    14101 tttcgctcgg cctatgtgaa gctcgccagc ggagatccaa gctgggcaaa cctgagcgct
    14161 ctgagttacc actattggac gcaaccgctt ccgaccaccc tcgcttggta tgcaaaccta
    14221 ttacctgcct gggttcagag agtctcttgt gcgctgatgt tcctggtcga gctcggcgct
    14281 ccagttttgc tcttcgtccc aaggccttgg gcgcgtcgga cggcggcggg atcgatcgct
    14341 gcgctcatgg ttctgatcgc gctcactggg aactacggtt tcttcaatct cctcaccatc
    14401 gtcctctgct tgccgttgct cgacgatcgc ctgcttctcc gactcactcc ggcgcgtttg
    14461 acccccagct cggattcctc gcgtagaatc ggatcgaacc cctggtcggg gcttgcgcct
    14521 gcgatggtga tcgcggtctc ggctgcggtc ttcttcacgg gcacgctgac tgggcgacct
    14581 ccggcctggc tcggcctggt ctatcggacg agcaccttca acaactatgg gctcttctcg
    14641 gtgatgacga ccgagcgccg ggagatcaac atcgaaggaa gctcagacgg ggagacctgg
    14701 aagccgtacg catttcgcta caagccagga ccgctcgacc gagccccggt ttgggtcgct
    14761 ccgcaccagc cgcggctcga ttggcagatg tggtttgccg ccctcggcga ctaccggcga
    14821 aacgattggc ttgcgaatct catgacccgc ttgctccaag gagagcggac tgtgctagcg
    14881 ctcatggaaa agaacccttt cgaagacgag cctccgaagc aggtgcgggc cgtgatctac
    14941 cgatacgagt tcaccgatcc ggaggaacgt cacgcgaccg gtaactggtg gaagcggagc
    15001 gaccgcttcc tttatgcgcc gatcctcggg gtcgaggtcg agccgactag tactgtagag
    15061 tgaaggagag cgaggcccag taggtgaaca ctttgtactt gccaggacaa ccggtgcgca
    15121 cgacctggtc gtcggagcag aggatcacgt ccgtgtcggt ccccggaatc tgggcggtgg
    15181 tgccgatgta ttcgttccca atggtgcccg tggtgaaggc gaaggccgtt ccgatatcga
    15241 gcatgtacct atccttgatg ctcgggtcgt tgcggtcgtc ccagtaaaag cccaggccgc
    15301 cggcgacgtt gccgctcaag cccggcggcg gcgtgaagta ctgggcgaac tcaccgctgg
    15361 tcgcagactt ggcgatgttc gcgcctagcc gaagagccaa gacgtccttg gtgaatcgat
    15421 actcgaatcc aactttcgcg ctccaggtgt tttcccaacc gaatggaacc acgatcgttt
    15481 gtagaaccgt tccatcgctg gacgcaatca ccgtttggtt cttgttcccg ctcttgtccg
    15541 ctccgtggaa ttgaaggcgc ccctcgaacg ctaagagcaa gtgactgttc gcctgatagg
    15601 agacgccgaa cgctattgcg tgagggatga accaatcggc ttccgattgc agcttcaaag
    15661 gctcggcccc tgcggcgagc tcagtctccg tctctcccgt cagcttgatc ttcgtcgcca
    15721 ttcgatacat cgcgctgatt cggacttttc ggtgcggctt gatggtgaac cccgcgcgta
    15781 gcgctggaat gccaacgccg ccaagatcgt tcttcacgcg cccatagccc acattgccga
    15841 acgcggcgcc gacattttga tagaggtcgg ccgtctgctt cgagaacggt agccgaagcg
    15901 cgagcccgag attgaacttc tcgtgcacgc gaaacgagaa tccggctgcg aattcgccga
    15961 cgaagaaggt cacgtccagg tcttcggctt gggggttgag gcactgtgga tcgctagtct
    16021 cggtagcggg tacccagttg tccggccgag gatcgggctg actcgagggg ttcggtgtca
    16081 cggccgcacc gtcgaggcac ttaaccccgc tgaacgacga gccatatccc gtctcgatgt
    16141 agatgccacc cgcaaacacc acgcggggag cgattcgccc ggcgatgaaa cccgacggca
    16201 agggtccaaa cccgagccca gaggagacac tggtgttcgg tccttgcacc ggcgccgttt
    16261 gattcacgag caggccggtg aagttgaccg tgaacccgag cttcgggatg ccctcgaggt
    16321 tggctgggtt gagggcgagg gcggcaggcc gatcgaggaa cgtgacgcct gtgagaccca
    16381 tcgcgatgct ccgcgtgtcg taggcggtcg gctccggatt cgcgctcgca aacgctggaa
    16441 tgagcagccc aagcgatagg cttgccgtca gtaaattgtg cttcattacg gggctcctcc
    16501 accaacgtgc gccctggggg ggccgccgca gcatacatgg aagcaccgct gataggtcaa
    16561 ccaaaaaagg ctcgatcggt gtacggatat gc
//
LOCUS       DASRZF010000053        26606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152 k141_38260,
            whole genome shotgun sequence.
ACCESSION   DASRZF010000053 DASRZF010000000
VERSION     DASRZF010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 26606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26606
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38260"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="VFG22_02395"
     CDS             <1..623
                     /locus_tag="VFG22_02395"
                     /inference="COORDINATES: protein motif:HMM:NF020130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HET6415119.1"
                     /translation="WIRCGMYKRVALVGAEVHSTALDFSDEGRDVTVLFGDGAACAIL
                     GPTDEEGRGIVDVEVHADGSGAKELWLEAPASAYKPRLTHEMIDNKTIYPSMNGRHVF
                     RWATAKMPEVSISVLERNGVKIEDLDLVVPHQANKRINEFVSQKLEIPPEKVVHNIQK
                     YGNTTAASIPLALSEAVQEGRAKKGDLILMPAFGSGFTWGAALVRL"
     gene            628..1641
                     /locus_tag="VFG22_02400"
     CDS             628..1641
                     /locus_tag="VFG22_02400"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HET6415120.1"
                     /translation="MQSKADRVAVVLNGNARQVTDELVESFDQLVGSGDLFLSRSLEE
                     AKGIALEIVDSEYPVVLTGGGDGTFVQMVTAITRAARERNQEPPRFGLLKLGTGNSLA
                     WALGAGTSRKGVFADLARLRHDSAHRDFRLIEVQGLLTPFAGAGFDALGLKHFHEVRS
                     LVRRLPWVGKLATGALSYAISIPTLTIPELALRPRMNVKIVNHGAAERLDRDGHPVGP
                     PVEKGEVIFDGACIAALVSTIPYWGFGVRVFPFAEDRPPDRFCLRVVATSPLHVAAHM
                     RSVWKGTYRDEKLIDVYAEDVEFLFDEPNAIEIGGDPAGMTRSMRARLDPDPVKLIDY
                     SAS"
     gene            1746..2435
                     /locus_tag="VFG22_02405"
     CDS             1746..2435
                     /locus_tag="VFG22_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HET6415121.1"
                     /translation="MGLLRNWKMLGAACVAVLLVTYGGASAEPVEGSVRGKSSANENP
                     DSGKQDYYWKVWNGALPEQPETDDLSDVLAVLTGKFTGPPIGCAFGFQGGDLSPSTLA
                     ARSGTTIRVENSDAFTHELSVKGLPGFTPLESGPGTARVISVPAGGPWSLGDRIYGHV
                     DGYLHSMRELVACTRVTANGTFRFDDVPPGPYSLRILRGADQVAVRRVTVPAGKSLQV
                     DALTMSESREK"
     gene            2439..4247
                     /locus_tag="VFG22_02410"
     CDS             2439..4247
                     /locus_tag="VFG22_02410"
                     /inference="COORDINATES: protein motif:HMM:NF041620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5187 family protein"
                     /protein_id="HET6415122.1"
                     /translation="MLSRLWYLVLALAVGGALAGAFLNKSAASREAEFQLEEQLRRDR
                     IEAELWMRLDARLRLDALATIAVEPEVRQTLRSSSGRAGTPGTAVRNRLSQKLNELNT
                     KLEEGKGDLVFALDTEGEVVATLDKNAMGSEQLDKMPLVQDALRGFMRDDVWVYNGEV
                     YRMAARPVFDGGRYVGAVVHGMLIRDELAKRLGSKLPNASLVFFFRDRLIASHVGGAN
                     APSSAELKNGMAAALAEMAASEGSVEVSERTRLIGSSIRGGAAHAQVGYIIGRRGSTV
                     GLGGLFANTFQEDVASLPWPVVVGIPLLLLLLGLFLLYLEHDKAVKILRKTSESLAKS
                     ELGSLPEGELRRHYRKIAENLNVALRAGVGFKEDASKRAAVDLDQILSNTRDSTDTGY
                     FGFGAEAPTAKQVSLDSEPELEVKPAASGTKDALPTAPPNGTPTARPNGASKARPQRS
                     PSSRPKRAPSSWPARAPSSRHQAVATIRHSEDNLTPAPDDPPTRPNAILDDYAGFDEP
                     EENTQVKHVPQELIEASAPAVQGEETHFREVFEQFVAMQKECGAPVSGLTFDKFVRKL
                     HAARDQIMKRHNAPAVHFTVYVKEGRAALKASPVKQ"
     gene            4606..4830
                     /locus_tag="VFG22_02415"
     CDS             4606..4830
                     /locus_tag="VFG22_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415123.1"
                     /translation="MRFGGILLLALAICASAACSGRTTEPSGPRGLTEADVGIPRERA
                     AKVLKVLESASDHNDRLQRMDQQLFAEDIK"
     gene            complement(4876..6942)
                     /locus_tag="VFG22_02420"
     CDS             complement(4876..6942)
                     /locus_tag="VFG22_02420"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6415124.1"
                     /translation="MQEATPSTVLGDFNDVTVKYFKETMRFLKEGDAFFVEALGADGK
                     RARFPVIYTFGVEPLQQYLVEIEPGRLQSFLLAWDTRPKGKGGQRWFHLQPEEYIEPG
                     DPLHWTGPSYNWNDNCADCHSTAVKKSYKRATKRYSTEFVEIDVGCEACHGPGSKHVE
                     LAKAAAAADQRLAANAGFDRRLPSPSERTWSFGSGKPIAELTAHQATDETETCAPCHS
                     RRADLGGESVRYHDRYRLAALDEELYFNDGQIKDEVFVYGSFLQSKMYAAGVICSDCH
                     DSHSAKLRVEGNALCGRCHRADVYDGPQHHFHATGTAGSLCTDCHMPQRTYMVHDERA
                     DHRFGVPRPALSAKIGAPDACTGCHQRKDAGWADRQIAQHFEIRSDHVFAEALDAARG
                     QRLGGEPGLIELVATGSGPAIVRATALLELRNLASPALPALLMRAANDSSPIVRRSVS
                     VAARDLPPEQTIEIVRPLLHDEIRSVRIEAVAALLGVHAGRWNPADRAALKSATGEYL
                     ESKAFNADRGAGLVDLAHVAMLAGDLASAESTLREALDVDPTFTAAYVNLADLYRSQE
                     RDEDADAILRQGLRVAADTASVEFALGLTLVRLDRRPEAVTHLARAYALRPEALRFGY
                     VYAVAQYDSGKEDAALRTLEQMRKRYPANRDILRLLAGYSQQLGRTEEARRLAAELEK
                     LGDSTN"
     gene            7238..8740
                     /locus_tag="VFG22_02425"
     CDS             7238..8740
                     /locus_tag="VFG22_02425"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HET6415125.1"
                     /translation="MELDRGNAVRLVRFLVGSFFRRVEVSGLEHVPTSGGGILVAWHP
                     NGLVDPALIISCFPRRVVFGARHGLFRWPMIGRLMKALGTVPIFRASDLRDADDDTRR
                     KANRESLDALAQAVCDGGFAALFPEGVSHDDPFLQELKTGAARLYYRAKQLCVENGAL
                     PVIIPVGLHYNDKTSFRSDVLVEFHAPLTLSPELLAPIAEDDDDAFRARSSELTQLLE
                     PVLQEVTHATESWELNQLMNRVRSLVRAERAKRVGANLKAADMLERAMGMARVWKAYY
                     ARLETDPEGVQALLERVFAYDQALVAVKLEDDDLDKPRPMESKWLPLLLVVQAAVVFL
                     VFPPVLFLGSMINAAPYFLVNLISRIASKQYKDTATIKLLTGLVLFPLAWLVAALLVG
                     LGQIELHNTFAGIPRAPWAAGITTFLIGAFGGALALVYFELFWRTWTSIKIRAKRLWG
                     SAIIRRLASERAALYEAVEEMRQGLDLPGDVLGDGRIGTRDFTLDPDDMP"
     gene            complement(8737..9291)
                     /locus_tag="VFG22_02430"
     CDS             complement(8737..9291)
                     /locus_tag="VFG22_02430"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HET6415126.1"
                     /translation="MKIYTKTGDSGETGLFGGARVSKASLRVEAYGEIDELNSTLGWA
                     RVEISDPNLDAILNRIQNDLFEVGAELGSTEDRKQKSTMPLIKEPQVEALERAIDKYE
                     QGPPALTTFVLPGGCESAARFHIARCVCRRAERALVTLGAQESLRGELFRYVNRLSDL
                     LFVLARYANHAAGVADIPWKGQAG"
     gene            complement(9288..10235)
                     /gene="fmt"
                     /locus_tag="VFG22_02435"
     CDS             complement(9288..10235)
                     /gene="fmt"
                     /locus_tag="VFG22_02435"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HET6415127.1"
                     /translation="MRTIFFGSPDFAVPCLDALHQISEIAVVISQPDRPAGRGLEMRP
                     PAVKKRALELGIEVWQPTKVRTADFASRLRALEADVAVVVAYGRILPRAVLDAPRLGC
                     VNVHASLLPRWRGAAPIQWAIVHGDRVSGVTLMQMDEGMDTGAIIAMESTPIQSTDDA
                     ESLSKRLSEMGAKLLRAQLPRYVAGELTAKQQDENAATMAPLLTKDHGRIDWNKPAEA
                     VHDQVRGMNPWPGAHTALHGRRIKVHRTVTSTLDPEGAPPGQVIALDADGILVACAEG
                     TLEIQELQESGRKRVDARAFATGRGVSVGDRFSSEGIEG"
     gene            complement(10244..10864)
                     /gene="def"
                     /locus_tag="VFG22_02440"
     CDS             complement(10244..10864)
                     /gene="def"
                     /locus_tag="VFG22_02440"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET6415128.1"
                     /translation="MRTCQTPLILRQAVLGARKIEVSTVLRGSTLGGFSLIRPILFYP
                     DKRLREPGKDVEVFDAELHQLIDDMAETMYAAPGVGLAAPQIGVSKRLFIVDVATDDD
                     EPSDLRVFINPEILATEGETTFNEGCLSFPGVREDIDRAERVRVRALDQDGNPFELEA
                     DGLLAIAIQHENDHLNGELMIDHLSVLRRRVLHRAMSKRAAAEAAQ"
     gene            10917..12647
                     /locus_tag="VFG22_02445"
     CDS             10917..12647
                     /locus_tag="VFG22_02445"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET6415129.1"
                     /translation="MADWDRTVDLLVMGSGAAGMGAAVRGHDLGLEVLLVEKSELYGG
                     NSAMSGGVCWVANNPGMMNEGIADSDEDGFRYLKHITKGEVPDERLWLYIRESKRVLE
                     YLHAKTHVRYIPLAKYTDYYPEAPGGRPGGRSMDPVPFDGAKLRDDLIRLRRPHPQSQ
                     ILGKFGITAKEAQAAIGVGFRTMMFMAWQMFLYMLRHFKRKRWGRDTKLCAGNSLMGR
                     LLYSLKERNVPLWLNTSVDELLFENGKVVGAVITREGQTLRLKTNRGVVLAAGGFSRN
                     LEMRKQYQRHPITTEWTAGTPSNTGDAIAMGRDAGGALDLMDEAWWTPVSLVPKSPFS
                     WVLVVEKSLPHGLFVNQDGKRFTNEAAPYIDVVNGMYDDVAKTGAANPRWFHIFDATY
                     RKSSIAGPIAPGKVMPDKQLPRRYRDGNFLYKAESLEELCEQLKIPFATVNATIARFN
                     EHAVKGEDPDFNRGWSAQDRYYGDPRVKPNCSLGPVETGPFYAVQIYPGDLGTKGGLV
                     TDEHSRVLKEDGRPIPGLYAAGNSSASVMGRTYPGAGGTIGPALTFGFLAAEDAARQP
                     TTRSEREAAA"
     gene            12644..13117
                     /locus_tag="VFG22_02450"
     CDS             12644..13117
                     /locus_tag="VFG22_02450"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HET6415130.1"
                     /translation="MSGRDWRLFGRIHTALYKTLGGRFVGSVGLGRRVLLLSTTGRRS
                     GAERTTPLVYMPYGKDLIVYPSNGGKETQPAWWLNLQSDPLARVQLGKEQRQVRARQA
                     TDTEYDAIWPAAEQYNPHWRDYARTVRRPIPLVILERVDVESRMAGGQALIDSRS"
     gene            complement(13052..15337)
                     /gene="priA"
                     /locus_tag="VFG22_02455"
     CDS             complement(13052..15337)
                     /gene="priA"
                     /locus_tag="VFG22_02455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HET6415131.1"
                     /translation="MNARASDSIDTRYVEVALPVPLRKVFTYDVPSGLQGALRPGSRV
                     AVSFSRRKLAGFVVSGREDLPSGVSRALPVAGLLESEPVFTPELLRFLDRAAKYYMHP
                     LGEVLRAAAPALPTGAMRRLRADGFLEATENLPGQRVAQPKTWKVTATGRDREGLRLG
                     ARQQRLLDRLEARESLLIDELRGEIKDPRAVIRSLSEKGLVAFEEVDAVSDPFFRTPV
                     QRDSPPAPTAAQEVAIAAITEAIVNRAGASFLLHGVTGSGKTEVYLRAIDEVRQAGRG
                     AILLVPEIALTPQLVGRFRARFGDDIAVLHSGLTIRQRDDAWQSLRQGRVRVAIGARS
                     ALFAPVADLGLIVVDEEHDPSFKQDEGFRYHARDMALLRAQYAGAVSVLGSATPSIET
                     YYRANQGGIRLLSLPTRATGAAMPEVEIVDLRRHRSGPTGHPLLSGPLHQAIGDCLET
                     GHQAIIFLNRRGFSPSIRCAACGALAECPACSVALTEHKAQGALRCHYCDFQRAVAQP
                     CIACGSPEYKRLGVGTEQLEKSIEESFPKARVARLDRDTASGEGVEAVLDRLRSGEVD
                     VLVGTQMVTKGHDIAGVTLVGVALADQSLAFPDFRASERTFQLLAQVAGRAGRAEAPG
                     KVVLQTYQPDHPAVRLAASHDYESFYDEEIRARDEVGYPPFARLVSVRVHAGAEADAR
                     GATQVLADAARQHPAVREGAVQVLGPAPAPLVRLRGRYHYRLLLKSSDRKLLRNVTAQ
                     LAARIDQGLPPSHATLDIDPL"
     gene            complement(15345..16415)
                     /gene="tsaD"
                     /locus_tag="VFG22_02460"
     CDS             complement(15345..16415)
                     /gene="tsaD"
                     /locus_tag="VFG22_02460"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HET6415132.1"
                     /translation="MRVLGIESSCDETAAAVVTEDGEVLTDVVASQIAMHGPYGGIVP
                     ELASRAHMQAIIPVIREAVAGLEGGLDAIDAIAVTEGPGLVGSLLVGVQVAKALAWSL
                     GKPLVGVNHLDGHLAAVYLRRSGRDEPVEAPELPFVGLLVSGGHTALYRVDSFADIEL
                     LAQTRDDAAGEAFDKAAKLLGLGYPGGPVIDRLAAVGDAAAVSFPLPMASRKTLEFSF
                     SGLKTALARRVQEHGVPEDEQSLADLCASFQGVIIESLVRKSILACKQEGLDRLVITG
                     GVAANRGLRARAREVCEVNGIRLFVPPPISCTDNAAMIAMAAVHRLRVGEHDDLSFAV
                     YSRDPDRRRGKFRRDGTLVERT"
     gene            complement(16415..16975)
                     /locus_tag="VFG22_02465"
     CDS             complement(16415..16975)
                     /locus_tag="VFG22_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415133.1"
                     /translation="MRRCCWITILLAVLGCNSLGEFSTGDDAVYRGDILGVNDPLNCP
                     DGIDCSFIRRGFPSGVTLGLTFEPDEQYVDPGTISTSGEPCGPTFQNTPLLSIPPLSH
                     DQLGQYEFPGGGRIRNYMFVVQPESGPLAGRDGMVFLSLLRNGRIEVRVVAGPGLVIC
                     DPANCEQINSGDCDYFGVFSLDREAL"
     gene            complement(16997..20005)
                     /locus_tag="VFG22_02470"
     CDS             complement(16997..20005)
                     /locus_tag="VFG22_02470"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET6415134.1"
                     /translation="MRLCHQCGASVDAAARFCSACGAPSDEQESGGAADPLVGRIVGG
                     SYLLQEVVGVGGMGRVYKAEQSTLGRTVAVKVIHPHLLGDEQTVARFYQEARASSRLN
                     HPNSVSIIDFGRTDDGILYLAMEFLSGKDLALVMHEEGPLAVERICNILINTLDALGE
                     AHELGIVHRDLKPENIILRPLRSGDDLVKVVDFGLATIVGKESSITKPGLVCGTPDYM
                     SPEQGRGDTVDGRGDLYALGVVLFELLVGHLPFIDDTPTRVVLRHLNDPVPDPREASP
                     NRNIPDRLADVTMKALAKKASDRYQLARDMQKALREAIDDLHVGRTSVTPCPNCGAPA
                     SLSQNFCGECGGRLAGAEPALGSNRSLRPSFYPALGSQRAFVGREDELRRLREHRHAA
                     SQGAHWVHVTGEPGVGKTRFLTEVAEIAAAEGDLVIGAGAHPTGAPVPYAAIRTILAE
                     LLEVDDSELARMANRGDELGSALAGAGVDEVMNPAGLLGGDGRSRTGAVAEALVCAVR
                     RARGRASSGRVMLIVDDLSYCDGLTQRTVIEFARALGNEPVLLVTAGDHDRGVLADEV
                     IGLQGFDSEEAERFLAGSFHLRLMSGLHQLTESIGGNVLPLHLEQLEALGVSPDDGPP
                     PRLADAILQRIERLDVEAQRLLQAAAVLGRTCELDALRAVSANDGLEGLDELAGNSLT
                     QLDGSKVSIVHPFIADLVAASIPAEARRAIHLRAYQVLSEARAPQEVRAVHAFHGVDP
                     VSSLVVLEKAGDASMARGDHLGAVLQYRRALELARRQTLESGDTVYDRAIATFSRKLG
                     EALARSGDASGADGVLREALDLTAPKSVDRAKMMLSLGRIASRRDRPRDAMRQYGQAL
                     ELVAGVEPSVESHLQVAIGRIRRIEGDPHHAANAYRRALELFAEINATADTIARTRLE
                     LADALTAAGDHEAAANELAGVEGDSRANGRTSLVARAVGMLGSIDELAGRARDAQVRY
                     EEAASLAAKAGDAPGYRRWLSASKTLRASI"
     gene            complement(20069..22126)
                     /locus_tag="VFG22_02475"
     CDS             complement(20069..22126)
                     /locus_tag="VFG22_02475"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET6415135.1"
                     /translation="MSRGASQEQAERIRGVAILDRLVVEGQVSPSRYDEIVLHAQRTE
                     CTAEEAILQLGLMSEAELLKYIAGLYRTRFVGSERLAKAGVEADLLAKVSRKLAKKLI
                     AFPILFDLKSRTLSVVAADVTDDDVRQQMQFATGARDVKVYVAREAAIRALISKHYDQ
                     DNLPFEMLLSGASSRQVRSSRDSDPVPGWGQVFEDKDGLLVRRRRAQSIEIEQAANNQ
                     LNEPSAEQAPPETQTRVSAGQTLIEQLSAPSQGGDLAVDPEVYLETLAVLVTLIEQLN
                     QDRGGHSMRVARVSQQVGERIRLTKPQIQGILIAAYLHDLGVSAESHVTLFDVAHEQS
                     NREAARRIYTAPLRLLESVTLPKTAVDALRHRFEQFDGQGFPERLSGKNIPLGARVLA
                     VAESYVELTQSSANAFGRRLAAQDACDALEAHKGLSFDPAVVDWLRHIVLDDGMKSQI
                     MSNRHRALIVDPDPEETAVLEVQFASHGYEVTIERSIEAALRCLNREKYDVVVSEVRL
                     ADGAGFDLLRRMREGSNAQVPLMFVTAERAQSSVNRGLELGAADYVVKPASAAVVAAK
                     ASRIISESRVKKPGGRGVSGSLQEMSLPDVIQILANGRKSGRLKVRGGTLQGELMFQD
                     GSIHDARLGDLAGAEAVYGILRLVEGDFELEPESRSVEDVIGIPTHHLLLEAMRRLDE
                     EQR"
     gene            22224..22739
                     /locus_tag="VFG22_02480"
     CDS             22224..22739
                     /locus_tag="VFG22_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415136.1"
                     /translation="MRRRLGIWLFVGLTTMGCASTGALQGGVFHGTQTSYRIGPVAPD
                     WTPVTVNHQNDLAWHNAAEGAVMHADSDCDPGLDIPLAALRNHLIIGFTEREVLEEEL
                     VPMDGREALRTHFRAKLDGVPRDMLLQILKKDGCVYDFALVTPPDASFDEALLDFDQM
                     LAGFTTATGRP"
     gene            22736..23557
                     /locus_tag="VFG22_02485"
     CDS             22736..23557
                     /locus_tag="VFG22_02485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6415137.1"
                     /translation="MTSASPSTRPRNFTSWADLQIRRLRKFVEEVGDATLLAGRTIRT
                     LFTTRFDSTEFIYQLEQLGVRSLAIAAATAIFVGIVMTIQFAVSMEAFGAKDTLGRVI
                     GVSEARELAPSLTALVVGSRIAAGIAAELGSMTVTEQVDAIRALGADPIRKLVVPRLV
                     ACIVIMPLITFIAFILGMISSMLVAALSYGIPMEFFSSTALDTLRIQDFVSGVSKTPF
                     FGFLIALLGCYFGLQTRGGTEGVGRSTTRAVVVVSISILMADALLTQIFVSFGTL"
     gene            23601..25751
                     /locus_tag="VFG22_02490"
     CDS             23601..25751
                     /locus_tag="VFG22_02490"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HET6415138.1"
                     /translation="MARWLIIWSLLGAAGCSSGGPQPLRSSRQDLSPSLMDAPALDLG
                     RLATTEDCADCHSDVASHWMHSGHAYASFDNPWYRASVDRFREQRGNEESRFCAGCHD
                     PLLLMSGDIDQEVSPGNALAYAGVTCLVCHSIESTRPDGNASFTLSDAPVLLPDPANP
                     EEIEAHRARLTMAPLRTAGLCGSCHRSFSGPMIGNENHLPGIDDLGDWASSAYAGAVQ
                     DQLVPLEEKSCQGCHMRPEVASEAEMAGAIDGRVSSHRWSASHTAMAAQLPGTKHAEQ
                     AVDELEGAVLVDIGPVRAGARRYLLPERSRLRAGERLLFDVLLQNHSTGHRFPGGVRD
                     MHDVWLEVEVRDASGTLLGVSQPTERGNDDVFILRATVLDAESSPEILHQVHRFSAPA
                     FDQTLPAHDARAVRYSMKLPMALNGPIRIEARLLHRKHSLEFQALACEASRTERGVEF
                     ARGAEKHGKIALDPCQEQPLTEIGAAAVWVGRGAAGRQAVGGAARPEVERLLTQALAL
                     LHAKQEEVHVAQPSIDRALRLARETRSPELEARALILRARLSSMQGRPDEADELLSGA
                     RALIGPSPVLDRVRGDAYARVWRWRDAVEAYRRVAEYSPLDSGAWRNLARAYGSLSDD
                     LNALAAADLGLRLAPRDESLLRSRALALESLGRPDAEQARNRWLAHRAPDAQAQLLAT
                     CEQEHQRCRRDRQPIPHYTLLPPRKAIHPGADPG"
     gene            25762..26004
                     /locus_tag="VFG22_02495"
     CDS             25762..26004
                     /locus_tag="VFG22_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415139.1"
                     /translation="MAKRYQKKSSTNKKREKKGHGPSRNTVGGGQTTGVMGNMVGGFR
                     RAVGSESSKDSKGGSLLWTAVLVTAAIAIVAWNFAR"
     gene            complement(26001..>26606)
                     /locus_tag="VFG22_02500"
     CDS             complement(26001..>26606)
                     /locus_tag="VFG22_02500"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HET6415140.1"
                     /translation="TVPAKVTLEQVEWLPDGEPNIVRCPDPVAAGQMVKLIESVRKDQ
                     DSIGGVAEIVARNVPAGLGEPVFDKLKADLGKALFSLPAVLGVEYGAGFAVATARGSE
                     NNDAFETDAEGTIRTRSNRHGGMLGGISSGMPILLRAAVKPTSSLPRSQDTVTASGDA
                     TTIRTTGRHDPCLLPRFVPMGEAMVALVLADHWLRWRAIHD"
BASE COUNT         4989 a         8395 c         8369 g         4853 t
ORIGIN      
        1 cgtggattcg ttgcggcatg tacaagcgag tcgccttggt gggcgccgaa gttcattcca
       61 cggcgctcga cttcagcgac gagggccgtg acgtgacggt tctattcgga gacggggcgg
      121 cctgcgcgat cctggggccc accgacgaag aaggccgagg tatcgtcgac gtggaggttc
      181 acgcggacgg gagcggtgcg aaggagcttt ggctcgaagc tccggcaagc gcctacaaac
      241 ctcgactcac ccacgagatg atcgacaaca agaccatcta cccatcgatg aacggccggc
      301 atgtgtttcg ctgggccacg gccaagatgc cggaggtgag catctcggtg ctcgaacgca
      361 atggcgtgaa gatcgaagat ctcgacttgg tcgtgcccca tcaggcgaac aagcgaatca
      421 acgagttcgt ttcgcagaag ctcgaaattc cgcccgagaa ggtcgtgcac aacattcaga
      481 agtatgggaa cacgaccgcc gcttcgattc cactcgccct gagcgaggcc gtccaggagg
      541 gccgggccaa gaagggtgac ctgattctca tgcccgcatt tggctctggt tttacatggg
      601 gcgccgcgct cgtgcggctc tgagcccgtg cagtccaagg ccgaccgcgt cgccgtcgta
      661 ctgaacggca acgccaggca ggtcaccgac gagctggtgg agagcttcga tcaactggtc
      721 ggtagcgggg acctctttct ttcgagaagc ttggaggaag cgaagggcat cgcgctcgag
      781 atcgtcgatt ccgaatatcc ggtcgtactg accggaggcg gcgacggcac attcgtgcag
      841 atggtcaccg ccatcacgag agcagcgcgc gagcggaatc aggaaccgcc gcgttttggc
      901 ttgctcaagc tgggtacggg gaactcgctg gcctgggcgc ttggcgcagg gacgtctcgc
      961 aagggtgtct tcgccgactt ggctcgactg cgccacgaca gcgcccaccg ggattttcga
     1021 ctcatcgaag tacagggtct actcacgcct ttcgcaggcg ctggattcga cgccctcggg
     1081 ctcaagcatt tccacgaggt tcgttctctc gttcgccgct tgccgtgggt cgggaagctc
     1141 gcgacgggtg cgctttccta cgcgatctcg atccccacac tcacgatccc cgagctcgcc
     1201 cttcgaccgc ggatgaacgt gaagatcgtc aaccacggcg cggcggaacg tctcgaccgg
     1261 gacggccatc ctgttggacc gcccgtcgaa aagggcgaag tgatcttcga cggcgcgtgc
     1321 atcgccgcgc tggtgtcgac gatcccctac tggggatttg gcgtccgggt cttcccgttc
     1381 gccgaagacc gccctccgga tcggttctgc ctccgggtgg ttgcgacgag ccctctgcac
     1441 gtggctgcac acatgcgcag cgtttggaag gggacgtatc gagacgagaa actcatcgac
     1501 gtctatgccg aagacgtcga gttcctcttc gacgagccca acgccatcga aatcggaggt
     1561 gatcccgcgg ggatgacacg ctcgatgcgc gctcgtttgg accccgatcc cgtcaagctg
     1621 atcgactact ccgcgtccta gcgttccgcg ctcggcccga agagacccgc gtcgtccgat
     1681 ttggatggaa aggccgaaat ttgttacctt tctctcagtg gggctgtaat aatacaggaa
     1741 taccaatggg gctacttaga aattggaaga tgttgggggc cgcgtgtgtc gccgttttgc
     1801 tcgtcaccta cgggggtgcg tcggccgagc cggtcgaagg atcggtccga ggcaagtcga
     1861 gcgcgaacga gaacccggac tccggcaaac aggactacta ctggaaggtc tggaacggtg
     1921 cgctcccgga gcaaccggaa accgatgacc tcagcgatgt gctcgcggtg ctaaccggga
     1981 agttcaccgg cccgccgatc gggtgcgcat tcgggtttca aggaggcgac ctgagtccgt
     2041 cgacgcttgc ggcgcgatcg ggaacgacca tccgggtcga aaacagcgac gcatttacgc
     2101 acgagctttc agtcaagggc cttcccggat tcaccccgct cgaatcggga ccgggcacgg
     2161 cgcgtgtcat ctccgttcct gcaggcggac cttggtcgct gggcgatcgc atctacgggc
     2221 acgtcgatgg ttacctgcat tcgatgcgcg agctcgtagc ctgcacgcgt gtgactgcga
     2281 acgggacgtt ccgattcgac gacgtcccgc cgggcccgta ctcgttgaga atccttcggg
     2341 gggccgatca ggtcgcagtc cgccgggtca cggtcccggc aggcaagtca ctgcaagtcg
     2401 atgcgctcac catgagcgaa agtcgggaga aatagccgat gctgtcgcga ctttggtatc
     2461 tagttctcgc tctggccgtc ggtggtgcgc tcgccggcgc atttctgaac aagtctgccg
     2521 cgagccggga ggcggagttt caactcgaag agcagctgcg ccgcgatcga atcgaggcgg
     2581 agctctggat gcgcctcgat gcccgccttc gtctggatgc attggcaacg atcgcagtcg
     2641 agcccgaggt tcgacagaca cttcgaagct cgagcggtcg cgccgggaca ccgggcactg
     2701 cagtgcgcaa tcggctcagc cagaagctga acgagctgaa tacgaagctc gaagaaggaa
     2761 aaggagacct ggtctttgcg ttggatacgg aaggcgaagt cgtcgctacg ctcgataaga
     2821 acgcaatggg ttcggagcag ctcgacaaga tgccgctggt ccaggacgcg ctccgtgggt
     2881 tcatgaggga tgacgtttgg gtctacaacg gagaggtcta ccgcatggcc gcgcgtccgg
     2941 tcttcgatgg cggtagatat gtgggggccg tcgttcacgg gatgctgatc cgggacgagc
     3001 tcgcaaagcg tctgggaagc aagctgccga acgcatccct ggtgttcttt tttagagatc
     3061 gcctgatcgc gtcgcacgta ggcggcgcaa atgctccgag cagtgccgag ctcaagaacg
     3121 gcatggcggc ggcgctcgcc gaaatggcgg ctagcgaagg ttcggtggag gtgagtgaac
     3181 gcacgcggct gatcggatcg tcgattcgcg gcggggccgc gcacgcgcag gtcggataca
     3241 tcatcggccg aagaggaagc acagtcggcc tcggcgggct attcgccaat acctttcagg
     3301 aagacgttgc atcactcccg tggccggtgg tggtcggaat tccgctgctg ctcttactgc
     3361 tgggcttgtt ccttctgtac ctcgaacacg acaaggccgt gaagatcttg aggaagacga
     3421 gcgaaagtct cgccaagagt gagcttggca gccttccaga gggcgagctt cgccgccact
     3481 accgcaagat cgccgagaac ctcaacgtgg cccttcgtgc aggggtcgga ttcaaggaag
     3541 acgcgtcgaa gcgcgcagcg gtcgacctcg accaaatcct gagcaacacc cgcgacagca
     3601 cggataccgg ctatttcgga ttcggtgccg aggcgcctac ggccaaacag gtatcgctcg
     3661 attcagagcc ggagctcgag gtcaagccag cagcgtcggg caccaaggat gcgcttccaa
     3721 ccgcacctcc aaacggtaca cccacggcga gacccaatgg tgcatcgaag gcgcgtcctc
     3781 aacgctcacc ctcttcgcgc ccgaagcgcg ccccttcgtc atggcccgcc cgtgcacctt
     3841 cttctcgcca ccaggcggtc gcgacgattc ggcacagcga ggacaatctc acaccggcgc
     3901 ctgacgatcc tccaactcgc cccaacgcca tactggatga ctacgctggc ttcgacgagc
     3961 cggaagagaa cacacaggtc aagcacgtgc ctcaggagct gatcgaagca tctgcgccgg
     4021 ccgtacaggg cgaagagact catttccgcg aagtattcga gcagttcgtt gcaatgcaga
     4081 aggaatgtgg cgctcccgtg agtggcctca cgttcgacaa gttcgtgcgc aagctccacg
     4141 ccgcgcgcga ccagatcatg aaacgtcaca acgctccggc cgtacacttc accgtctacg
     4201 tcaaagaagg gcgtgcggcg ctaaaagcca gtccggtcaa gcagtgagtt gaccgccggg
     4261 gatggcggct ctaccttagc aaatagtccc tctgttgttg caggtgagga aggagtaaag
     4321 aatccctgac taggtcgatt agggagctac aacgtacgtg gtgtgcggac ccggctagcg
     4381 tcatcggggg gcctgaagcg tgcgacaacc gctcccacct actagtaaat tgtaggggtt
     4441 cgactcaaca ttagatccca cggcaacgac ggtcaggact gtcttatgaa gccccgcgaa
     4501 ctcgacgctt ttcgtcgaca gcggggcttc ggggcttaat cgaccaagca ggctgatcga
     4561 cccgccgaat gggcagcgtg gggggcgatc ccctagactg tgttcatgag gtttggggga
     4621 attctcttgc tcgccctagc catctgcgcc tcggctgctt gttcgggacg cacgacggag
     4681 ccgtcgggtc ctagggggct cacagaagcg gatgttggca tccccaggga gcgcgcggcc
     4741 aaggtcctga aggtcctcga gtctgcaagc gatcacaatg atcgcctcca gcgcatggac
     4801 caacaactct tcgccgaaga catcaagtag agcccggcat cttcatcccc tgcccggggg
     4861 ccacgatcgc gaagctcagt tcgtcgagtc gccgagcttc tcgagctcgg ccgcaagtcg
     4921 ccgggcctct tccgtgcggc ctagctgctg actgtacccg gcgagcaatc gaagaatgtc
     4981 gcggttggct ggatagcgtt tgcgcatctg ctcgagtgta cggagcgccg cgtcctcttt
     5041 ccccgagtcg tactgcgcaa ccgcgtaaac atagccgaac cgcagggcct cggggcggag
     5101 cgcgtatgct cgtgcgaggt gcgtcacggc ttcggggcgg cgatcgagtc gaaccaacgt
     5161 gagacccagt gcgaactcca ccgatgcggt gtccgctgcg acgcgaagcc cttggcgaag
     5221 aatcgcgtcg gcgtcctcgt cgcgctcttg acttcgatag aggtcggcga ggttcacgta
     5281 ggccgccgtg aacgtgggat cgacatctag cgcctcgcga agcgtgctct ctgcgctcgc
     5341 taggtcgccg gcgagcatcg cgacgtgagc caggtcgacc aagcccgcgc cacggtcggc
     5401 attgaaggcc ttggactcga ggtattcccc ggtggcgctc ttcagcgccg ctcggtctgc
     5461 ggggttccac ctgccagcat gaacgccgag aagcgcggcg acggcttcga ttcgcaccga
     5521 gcgaatctcg tcgtggagaa ggggtcggac gatctcgatc gtttgctcag gcggaagatc
     5581 tcgcgcagcg actgaaaccg aacgtcgcac gatcgggctc gaatcgttgg ccgcacgcat
     5641 cagtagtgca gggagcgccg gcgaagctag gttgcggagc tcgagcaagg cggtggcccg
     5701 cacgatcgcc gggccggaac cggtcgcgac gagctcgatc agtccaggct cgccccccag
     5761 tcgctggcct ctggcagcgt ccaaagcctc ggcgaagacg tgatcggaac ggatctcgaa
     5821 gtgttgggct atttggcgat ccgcccagcc ggcgtctttg cgctgatggc atccggtgca
     5881 cgcgtccggt gctccaatct tcgcgctgag cgccgggcgc gggacaccaa aacggtggtc
     5941 tgcgcgttcg tcgtgcacca tgtaggtgcg ctggggcatg tggcagtccg tgcaaaggct
     6001 cccggccgtt cccgtagcgt gaaaatggtg ctgggggccg tcgtagacat cggcgcggtg
     6061 acagcggcca cagagcgcat tgccttccac acgtagcttc gcgctatgcg aatcgtggca
     6121 atcgctacaa atgacgccgg ccgcgtacat cttgctctgc aagaacgagc cgtagacgaa
     6181 gacttcgtct ttgatctgtc cgtcgttgaa atacagctcc tcgtcgagag cagccagccg
     6241 atagcgatcg tggtagcgga cgctctcgcc acctaggtcc gcgcgacgcg aatgacatgg
     6301 tgcacaggtc tcggtttcgt cggtcgcctg gtgtgcggtc agctcggcaa tgggcttccc
     6361 cgaaccgaat gaccaggttc gttcggaagg cgagggaagg cgacggtcga agccggcatt
     6421 cgcggctagg cgctgatcgg cggctgcagc cgctttcgca agctccacgt gcttcgagcc
     6481 cggaccgtgg caagcctcgc aacccacgtc gatctcgacg aactccgtgg agtagcgctt
     6541 ggtcgcgcgc ttgtagctct tcttcaccgc cgtcgagtga cagtcggcgc agttgtcgtt
     6601 ccagttgtag ctcgggcccg tccaatgcag cggatcgccg ggctcgatgt actcctcggg
     6661 ctgaagatga aaccatcgct gaccgccctt ccctttgggt cgcgtgtccc atgcgaggag
     6721 aaacgactgg aggcggccgg gctcgatctc gacgaggtac tgctggagcg gctcgacgcc
     6781 aaacgtgtag atcactggaa agcgggcgcg ctttccatcg gcgccgagtg cctccacgaa
     6841 gaatgcgtcg ccctccttga ggaaccgcat cgtctccttg aaatacttca cggtcacgtc
     6901 gttgaagtcg ccaagcacgg tgctcggcgt cgcttcttgc atggacaaat cgtggtgcga
     6961 cccgcgccac gcctcctgct cggcctgatg gcaggaagcg cactgcgggg ttcccacgta
     7021 cttcgtgggc tcttcctcgt ctgcaaccgg cgagcttgtt gaaagcggat tcggagctgg
     7081 cgtcgccggg acctcttcag tgcttctccg gcacgcgcct gacgagaggg cgatcgcgat
     7141 caggaagccg taagccaagc cgctccgcat aacccgaccg atctagcgcg tattgaaaaa
     7201 ggggacaggt ctcttttcaa agggatagta tgccggtgtg gagctcgatc ggggaaacgc
     7261 ggttcgcttg gtgcggtttc tcgtgggctc gttcttccgc cgggtggagg tgagcggcct
     7321 cgagcacgtg ccgacatctg ggggcggcat tctggtcgcc tggcatccga acgggctggt
     7381 cgaccctgcc ttgatcatca gttgctttcc gagacgggtc gtcttcgggg cgaggcacgg
     7441 gctctttcgc tggccgatga ttgggcgact gatgaaggcg ctgggcacgg ttcccatttt
     7501 tcgcgcgtcc gatttgagag acgcggacga cgacacgcgg cggaaagcca atcgagaaag
     7561 cctggatgcc ctggcgcaag cggtttgtga tggtggattt gccgcgctct tcccggaagg
     7621 cgtgagtcac gatgatccgt tcttgcaaga gctcaagaca ggcgctgcgc gtctttacta
     7681 tcgcgccaag caactctgcg tagagaacgg agcgctaccg gtgataattc cggtgggcct
     7741 tcactacaac gacaagactt cgttccgctc ggacgtactg gtcgaatttc atgcgcccct
     7801 gacactttcg cccgagttgc tagcgccgat cgcggaagac gacgacgacg cgtttcgcgc
     7861 gaggagcagc gagctcaccc agcttctcga accggttctc caagaagtca cccatgcgac
     7921 ggagagctgg gagctcaatc aactgatgaa tcgcgtgcgc tccttggtca gggcggaaag
     7981 ggcgaagcga gtcggcgcga acctcaaagc tgctgacatg ctcgaacgcg ccatggggat
     8041 ggcgcgggtg tggaaagcct actacgcacg gctcgaaacg gatcccgagg gtgtccaagc
     8101 actgctcgaa cgcgtttttg cctacgatca ggcgctcgtc gcagtgaagc tcgaagacga
     8161 cgatctcgac aagccgcgtc ccatggaatc caaatggctt ccgctgctcc tcgtcgtcca
     8221 agccgcggta gtcttcctgg tctttccgcc ggtcctgttc ctgggttcga tgatcaacgc
     8281 tgcgccttat ttcctggtga acctgatctc gcgcatcgct tcgaaacagt acaaggacac
     8341 cgcgacgatc aagctgctca ccggactggt tctgtttccc ttagcctggc tcgtcgctgc
     8401 tctgctggta ggcctcggac agatcgagct ccacaacaca ttcgcgggga ttccacgcgc
     8461 gccctgggcg gcaggcatta cgactttcct gatcggcgca ttcggtggag cgctcgcgtt
     8521 ggtctatttc gagctcttct ggcgcacctg gacgtcgatc aagattcgag caaagcgtct
     8581 atggggatcg gcgatcattc gtcggctcgc ctcggagcgg gcggcgcttt acgaggcagt
     8641 cgaggaaatg cgacaaggcc tcgacttgcc cggagacgtg cttggcgatg gtcgaatcgg
     8701 cacgcgtgac ttcacgctgg atccggacga catgccttag ccggcctgac ccttccaagg
     8761 aatgtccgcc acgcccgccg cgtgattcgc gtaccgagcc aggacgaaaa gcaggtcgct
     8821 caggcgattg acgtaccgaa atagctctcc gcggaggctt tcttgcgcgc ccagcgtcac
     8881 caaggcgcgc tctgcgcgac ggcagacgca ccgagcgatg tgaaaacgcg ctgcgctttc
     8941 gcacccaccg ggcaagacga atgtcgtaag cgctggcgga ccttgctcgt atttgtcgat
     9001 cgcgcgctcg agggcctcga cctgaggctc cttgatcaac ggcatcgtgc tcttctgttt
     9061 gcggtcctcg gtcgaaccga gctcggcacc gacttcaaac aagtcgtttt gaatgcgatt
     9121 caggatcgca tcgagatttg ggtccgaaat ctccaccctc gcccaaccca aggtgctgtt
     9181 gagctcgtcg atttcgccgt acgcttcgac gcgaagcgac gctttcgaaa ccctggcgcc
     9241 gccgaacagc ccggtctcgc cggagtcgcc cgtcttcgta tagatcttca tccctcgatc
     9301 ccttcgctcg aaaaccgatc gcccaccgag actccgcgcc cggtggcaaa ggcgcgggcg
     9361 tcgactcgct ttcgcccgct ctcttgcagc tcctgaatct ccaacgtgcc ctctgcgcag
     9421 gctaccagaa tcccatctgc atcgagcgcg atgacttgcc ctggcggcgc tccctccgga
     9481 tcgagggtcg acgtaactgt gcgatgtact ttgatacgcc gaccgtggag agccgtgtgg
     9541 gcgccaggcc atgggttcat gccgcggacc tggtcgtgca cggcctccgc cggcttgttc
     9601 cagtcgattc ggccgtgatc tttggtgagg agaggtgcca tcgtcgctgc gttctcgtct
     9661 tgctgctttg ccgtcagctc gccagccacg tagcgcggca actgcgctcg caagagcttg
     9721 gcacccatct ctgaaagccg cttgctcagc gattcggcgt catccgtact ctgaatcgga
     9781 gtcgattcca tcgctatgat cgccccggtg tccattcctt catccatctg catcaaagtg
     9841 acaccgctga cccgatcgcc gtgcacgatc gcccattgaa tcggcgcggc accacgccaa
     9901 cgcgggagca atgacgcgtg cacgttcacg cagcccagac gtggcgcgtc gagcaccgca
     9961 cggggaagaa tgcggccata tgccaccacg accgccacat cggcctcgag ggctcgcagc
    10021 cggctcgcga aatcggccgt gcggaccttc gtgggttgcc aaacctcgat cccgagctcg
    10081 agcgcgcgct tcttcaccgc tggcggacgc atctcaagcc cacgccccgc cggccgatcg
    10141 ggctgggaaa tcaccacggc aatttcactg atctggtgaa gtgcgtcgag gcagggcacc
    10201 gcgaagtccg ggctgccgaa gaagatggtt ctcacggggg ccgttactgg gcggcttcgg
    10261 ccgcagcgcg cttgctcatc gcgcggtgca acactcggcg gcgcaagaca ctgagatggt
    10321 cgatcatgag ctcaccgttg agatgatcgt tctcgtgttg aatggcgatc gccagcagcc
    10381 cgtccgcctc gagctcgaac ggattgccgt cctggtcgag cgcgcgaact ctgacgcgtt
    10441 cagcccgatc gatgtcttcg cggacccccg ggaaagacag gcagccttcg ttgaaggtcg
    10501 tttcgccctc cgtggcgagg atctccgggt tgatgaagac gcgaaggtcg ctcggttcgt
    10561 cgtcgtccgt tgccacatcg acgatgaaca gccgcttcga caccccgatc tgaggggcag
    10621 ccagtccgac ccccggtgcc gcgtacatgg tttccgccat atcgtcgatc aactgatgaa
    10681 gctcagcatc gaacacctcg acatccttgc cgggctcgcg gagacgtttg tcgggataga
    10741 agagaatggg gcgaatcaaa ctgaaacctc cgagcgtcga gcctctcaag acagtggaaa
    10801 cctcgatttt tctagcgccc agcaccgctt gccgcaagat caggggcgtt tgacaggtcc
    10861 gcatcgctag aacatgttct atttttcgaa ccggcctgaa gtaaaggagc gccgcaatgg
    10921 cggattggga ccgtacagtc gatttactcg tgatgggaag cggcgccgcc ggcatggggg
    10981 ctgccgttcg cgggcacgac ctcggactcg aggtcctgct ggtcgaaaag agcgagctct
    11041 acggcggcaa ctcggcgatg tccgggggtg tctgttgggt ggccaacaac ccaggcatga
    11101 tgaacgaggg catcgcagat tcggacgaag acggttttcg ctacttgaag cacatcacca
    11161 agggtgaggt gcccgacgag cgactctggc tgtacatccg cgagagcaag cgagtgctcg
    11221 aatacctgca cgcgaaaacg cacgtccggt acatccctct cgcgaagtac accgactact
    11281 acccggaagc tcccgggggc cgccccggtg ggcgctcgat ggatccggtg cccttcgatg
    11341 gtgcgaagct cagggacgac ctgattcgac ttcgcaggcc tcacccgcaa tcgcaaatcc
    11401 tcgggaagtt cggaatcacg gccaaggagg cacaggctgc gatcggcgta ggcttcagga
    11461 cgatgatgtt catggcctgg cagatgttct tgtacatgct gcgccacttc aagcggaagc
    11521 gctgggggcg cgacaccaag ctctgcgcgg gcaactccct gatggggcgc cttctctact
    11581 cgttgaagga gcgaaacgtc ccgctttggc tcaataccag cgtcgacgag ctgctgttcg
    11641 aaaatggcaa ggtcgtcggt gccgtgatca ctagggaagg tcaaacactg cggctcaaga
    11701 cgaaccgcgg cgtagtgctg gcggcgggcg gattttcgcg gaacctcgag atgcgaaagc
    11761 agtaccagcg gcatccgatc acgacggaat ggaccgcggg aacgccatcg aacacggggg
    11821 atgcgatcgc gatggggcgg gatgctgggg gcgctctcga cctgatggat gaagcctggt
    11881 ggacgccggt ttcgctcgtg cccaagtccc cgttttcctg ggtactggtc gtcgaaaaga
    11941 gcttgccgca cggattgttc gtcaatcaag acggcaagcg gttcaccaac gaggccgcgc
    12001 cgtatatcga cgtggtcaac ggcatgtacg acgacgtagc caagaccggc gctgccaatc
    12061 cgcgctggtt ccacatcttc gacgccacgt atcgcaagtc gtcgatcgcg ggaccgatag
    12121 cgcccggcaa ggtcatgccg gacaagcaac tccctcgacg gtaccgagac ggaaacttcc
    12181 tctacaaagc cgagtcgctc gaagagcttt gcgagcaatt gaagatccct tttgcgacgg
    12241 tgaacgcgac cattgcgcgc ttcaacgagc atgcagtgaa aggggaggac ccggacttca
    12301 atcgcggctg gagcgcacag gatcgctact acggcgaccc acgggtgaag ccgaactgct
    12361 cgctcggtcc ggtcgagacc ggtccattct acgcggtgca gatctacccg ggcgacctcg
    12421 gaaccaaagg agggctcgtc acggacgaac atagccgcgt gctcaaagaa gacggccggc
    12481 caattccggg cctctatgcc gccggcaact cctcggcctc ggtcatggga cgcacctatc
    12541 ctggggccgg gggcacgatc ggcccggccc tgacctttgg cttcctcgcg gccgaagatg
    12601 ccgcgcgcca acccacgact cggagcgagc gggaagcggc ggcgtgagcg gacgcgattg
    12661 gcgtctcttc gggcgtatcc acacagcgct ttacaagacg ctgggcggcc gcttcgtcgg
    12721 aagcgtaggg cttggacgaa gagtcctgct tctcagcacg acgggtcgca ggagcggcgc
    12781 ggaacgaacc accccgctcg tctacatgcc gtacgggaag gatctgatcg tctacccatc
    12841 caacggcggc aaggaaacac agcccgcctg gtggctaaat ctccagtcag atcccctcgc
    12901 ccgcgtccag ctcggcaagg aacagcgtca agtccgcgcg cgtcaggcga cggatacgga
    12961 gtacgacgcg atctggcctg cggccgagca gtacaaccca cactggcgcg actatgcgcg
    13021 aaccgttcgg cgtcccatcc ctctcgtcat actagagcgg gtcgatgtcg agagtcgcat
    13081 ggctgggggg caggccctga tcgattcgcg cagctagctg ggcggtgacg tttcgaagca
    13141 gctttcggtc gctcgatttg agcaggaggc ggtagtggta gcgcccccga aggcgcacga
    13201 gaggcgccgg cgccgggccc aagacctgca ccgccccttc tctgaccgct gggtgctgcc
    13261 gggcggcgtc cgcgagaact tgggtcgcac ccctcgcgtc ggcctcggca cccgcatgaa
    13321 ctctcaccga gaccaggcgg gcaaagggtg gataacccac ttcgtcacgg gcgcgtatct
    13381 cttcgtcata gaagctttcg tagtcgtggc tagccgccaa gcgcacggcc gggtgatcgg
    13441 gctgatacgt ttgcaggacc actttgcctg gcgcctcggc gcggcctgcc cgaccggcga
    13501 cctgcgctag aagctgaaac gttcgttccg acgcgcgaaa atccggaaat gcaaggcttt
    13561 ggtcggcgag cgcgacgcct actagggtga cacccgcaat gtcgtggccc ttggtgacca
    13621 tctgcgtgcc caccagcacg tcgacttctc cgcttcggag ccgatcgagc accgcttcga
    13681 cgccctcacc cgatgccgtg tcacgatcga gtcgagccac gcgggccttt gggaagctct
    13741 cttcaatcga cttctcgagc tgctccgtgc caacgccaag gcgcttgtac tccgggctac
    13801 cgcaggcgat gcaaggctgt gcgaccgctc gttggaagtc gcaataatgg catcgcaacg
    13861 cgccctgcgc cttatgctcc gtgagagcga cgctgcaggc tgggcactcc gccaaggcgc
    13921 cacacgcggc gcagcgaata ctcggcgaaa agccccgtcg gttgagaaag atgatcgctt
    13981 ggtgcccggt ctctaggcag tcgccgatcg cctggtgcaa ggggccggaa agaagcgggt
    14041 ggcccgtggg tccgctccga tgccttcgaa ggtcgacgat ctcgacttca ggcatcgccg
    14101 caccggtggc gcgtgtcgga agggagagca atcgaatgcc tccctggttg gctcgatagt
    14161 aggtttcgat cgaaggcgtg gcgcttccga gcacagagac cgcgcctgcg tattgcgccc
    14221 ggagcaatgc catgtcccgc gcgtggtagc gaaagccctc gtcctgcttg aacgagggat
    14281 cgtgttcctc gtcgacgacg atcagaccga ggtcggcaac cggcgcaaac aaagcggagc
    14341 gcgcgccgat cgcgactcgc accctcccct gacgaagcga ctgccatgcg tcatcacgtt
    14401 gccgtatcgt gaggccgctg tgcagcacgg cgatgtcgtc gccgaacctc gcacgaaaac
    14461 gcccaacgag ttggggtgtg agcgcaatct ccggcaccag caatattgcg cctcgacccg
    14521 cttggcgcac ctcgtcgatc gcgcggaggt agacttccgt ctttccggaa ccggtcaccc
    14581 cgtgcaacag aaacgaggcg ccggcccgat tcacgatggc ctcggtgatc gcggcgatcg
    14641 cgacttcctg ggccgccgtt ggcgcaggtg gcgagtcgcg ctgcacaggt gtgcgaaaga
    14701 acgggtcgct gacggcgtcg acttcttcaa acgcgacgag ccctttttcc gaaagggagc
    14761 gaatcacggc gcgaggatcc ttaatttcgc cacgaagctc gtcgatcaag agcgattcac
    14821 gagcctcgag gcggtcgagt aggcgctgtt gccgcgcgcc gagacgaaga ccttctcgat
    14881 ctcgacccgt ggccgtcact ttccacgtct tgggctgcgc cacgcgctga ccaggcaggt
    14941 tttccgtggc ctcgagaaag ccatcggccc gcaaccgacg catcgccccg gtcggcaatg
    15001 ctggggccgc agcgcgaagg acctcgccaa gcgggtgcat gtagtacttc gctgctcggt
    15061 ccaggaaccg cagaagctcg ggagtgaaaa cgggttccga ttcgagaagc cctgcgaccg
    15121 gtagcgcgcg agagacgccc gagggcaggt cctcacggcc gctaacgacg aagcccgcga
    15181 gcttgcgtcg gctgaaggag acggccactc ggcttcccgg acggagggcg ccctgcaatc
    15241 cgctcggcac gtcgtaggtg aagaccttcc tgagtggaac cggaagcgcc acttccacgt
    15301 agcgagtatc aatcgaatcg gacgcgcgtg cgttcatggc acgcctatgt gcgctcgacc
    15361 agtgttccat cccgccggaa cttgccgcgg cggcggtccg ggtctcgaga gtagacggcg
    15421 aaggagagat catcgtgctc gcccacgcga agacggtgta cggcggccat cgcgatcatc
    15481 gcagcgttgt ccgtgcaaga gattggcggc ggcacgaaga gcctgatgcc gttgacctcg
    15541 cagacttccc tagcgcgtgc gcgtagacca cgattggcgg cgaccccgcc agtgatgacc
    15601 agccgatcga gcccctcttg cttgcaagcg aggattgatt tccgcaccag actttcgatg
    15661 atgaccccct gaaacgaagc gcaaaggtcg gcgagactct gctcgtcctc ggggactcca
    15721 tgctcttgga ctcggcgggc gagtgccgtt ttgagacccg agaagctgaa ttcgagtgtt
    15781 ttccgggatg ccatgggaag aggaaacgaa accgcagcgg catcaccgac cgcagccagc
    15841 cggtcgatca cgggcccgcc cgggtatccg aggccgagga gcttggcggc cttgtcgaag
    15901 gcctcgcccg ccgcgtcgtc gcgcgtctgc gcgagtagct cgatgtccgc aaagctgtcg
    15961 acgcggtaga gggccgtatg cccaccgctc accagcaaac cgacgaacgg caactcgggc
    16021 gcttcgacgg gctcgtctcg tccagatcgt cggaggtaaa ccgcagcgag atggccgtcg
    16081 aggtggttca cccccaccag aggctttccg aggctccagg ccagagcctt ggcaacctgc
    16141 accccgacga gaagtgatcc aaccaaccca ggaccttcgg taaccgcaat cgcatcgatg
    16201 gcgtcgagcc ccccttcaag cccagcgacg gcctcgcgaa tcaccggaat gatcgcctgc
    16261 atgtgcgcac gtgaggcgag ctctggaacg atgccgccgt acggaccatg catggcgatc
    16321 tgactcgcca ccacgtcggt caagacctcg ccgtcttcgg ttaccaccgc cgcggccgtc
    16381 tcgtcgcaag agctctcgat cccgaggacg cgcatcaaag cgcctcccga tccagactga
    16441 acacaccgaa gtaatcgcag tcgcccgaat tgatctgctc gcagttggcc gggtcgcaaa
    16501 tgaccagacc cggaccggcg accacccgca cctcgatacg gccatttcgc aggagcgaca
    16561 agaacaccat cccatctctg cccgcgagcg ggccggactc aggctgaacg acgaacatgt
    16621 agtttcggat cctgccgccg ccaggaaact cgtattggcc gagctgatcg tgcgagaggg
    16681 gcggaatcga gagcaagggg gtgttctgga acgtggggcc gcaaggctcg ccactggtcg
    16741 agatggtgcc gggatcgaca tactgctcgt caggctcgaa cgtgagaccc agcgtgaccc
    16801 cggatggaaa tccgcggcgt atgaaagagc agtcgatccc atcggggcaa ttgaggggat
    16861 cgttcacccc caagatgtcg cctcgataga ccgcatcatc tcccgtgctg aattcaccga
    16921 gcgagttgca gccgaggacc gcgagcagaa ttgttatcca gcagcagcgc cgcatgcggc
    16981 gaagcatagc agagtgctag atgctcgctc ggagcgtttt gctggccgaa agccaacggc
    17041 gatagcccgg cgcatctccg gccttcgcgg ccagagaggc ggcttcctcg tatcgaacct
    17101 gcgcgtcccg cgctcggccg gcaagctcat cgatcgagcc cagcatgccg accgctcgcg
    17161 cgaccaggct ggtccgtcca ttcgctcggc tatcgccctc tacaccagcg agctcgttcg
    17221 ctgcggcctc atggtcgccc gctgcggtca aggcgtcggc tagctcgaga cgggttcgcg
    17281 cgatggtatc cgcggtcgcg ttgatctccg cgaagagctc gagcgcgcgc ctgtaggcat
    17341 tggcagcgtg atgcgggtct ccctcgatgc ggcgaatgcg cccgatcgcg acctgcagat
    17401 gggactcgac cgaaggctcg accccggcga ccagctcgag cgcttgcccg tactgacgca
    17461 tggcgtcccg agggcgatct cttcgcgacg cgatgcgtcc gagcgagagc atcatcttgg
    17521 cgcggtcaac gctcttagga gcggtgagat cgagcgcttc tcggagcacg ccgtcggcgc
    17581 ccgatgcgtc gcctgatcgt gcaagcgctt cgccgagctt ccggctgaag gtcgcgatcg
    17641 cgcgatcgta gacagtgtcg ccgctctcca aggtctgacg ccgggccagc tccaaggcgc
    17701 gtcgatattg cagaacggct ccgagatgat ctcccctcgc catcgacgca tcgccggcct
    17761 tttcgagaac gaccaagctg ctcaccggat ctacgccgtg gaatgcatga acagcacgaa
    17821 cttcctgagg cgcgcgagct tcactcaata cttggtaggc acgaagatga atcgctctgc
    17881 gtgcttcagc cggaatggaa gccgctacca gatcggcgat gaaaggatgg acgatgctca
    17941 ccttcgaacc gtcgagctgc gtgagcgaat tgccggcaag ctcatcgagg ccttccagcc
    18001 cgtcgtttgc cgaaacggcc cggagcgcgt cgagctcgca cgtccggccg aggacggcgg
    18061 cggcttgaag taggcgctgc gcctcgacgt cgagacgctc gatgcgttga agaatcgcgt
    18121 ctgccaaacg cggcggcgga ccatcgtcgg gagaaacccc aagcgcttcg agttgctcga
    18181 ggtgcagggg taacacgttg cctccgattg attcggtaag ctggtgcaga cccgacatca
    18241 agcgcaggtg aaacgagcct gccaagaagc gttccgcttc ttccgagtcg aagccctgca
    18301 gtccaatgac ctcgtcggcg agcacgcctc ggtcgtgatc gcctgcagtc acgagcaaaa
    18361 ccggctcgtt gccaagcgcc ctggcaaact cgatcacggt gcgctgcgtg agtccgtcac
    18421 aatacgagag gtcatcgaca atgagcatca cgcgaccact cgacgcccga ccccgcgcgc
    18481 gtcgcacggc gcacacgagg gcttcagcga cggcgcccgt tcgcgacctg ccgtcgccac
    18541 cgagcagacc cgctggattc atcacctcgt cgacgcccgc ccctgccaac gccgagccaa
    18601 gctcgtctcc gcgattggcc atccgcgcca gctccgaatc gtctacttcc agaagctccg
    18661 cgaggatcgt gcgaatcgca gcgtagggaa ccggggcccc ggtcggatgc gcgcccgctc
    18721 caatgacgag gtctccttcg gccgccgcga tctcggcgac ttcggtgagg aagcgggtct
    18781 tcccgacgcc aggctcgccc gtgacgtgaa cccagtgcgc gccctgtgac gctgcgtggc
    18841 ggtgttcgcg tagtcgtcga agctcgtctt cgcgcccaac gaaggctcgc tgcgagccca
    18901 gagctggata gaaggagggt cgcagcgaac ggttgctgcc cagagccggc tcggcgccgg
    18961 caagccgtcc accgcactca ccacagaaat tttgcgagag cgacgcgggg gcaccgcaat
    19021 tcgggcaggg cgtcacggac gtgcgaccaa cgtgcaagtc gtcgatcgcc tcgcgcaacg
    19081 ccttctgcat gtcgcgggcg agctggtagc ggtcgctcgc cttcttcgcg agcgctttca
    19141 tcgtcacatc ggcgaggcga tcgggaatgt tgcgattggg gctcgcttcg cgcggatcgg
    19201 ggaccgggtc gttgaggtgt cgcagaacga cccgcgtggg cgtatcgtcg atgaacggca
    19261 gatgaccgac caagagctcg aaaaggacga cgccaagcgc gtagaggtcg cccctgccat
    19321 ccaccgtgtc gccccgtccc tgctcagggg acatgtaatc gggcgttccg caaacgagac
    19381 cgggtttggt gatcgagctc tccttgccaa cgatggtggc aagcccgaag tcgacgacct
    19441 tgaccagatc atctccggat cgaagcggac gcagaatgat gttctcgggt ttcaggtcac
    19501 gatgaacgat gccgagctca tgcgcttcgc ccagcgcgtc caacgtattg atcaagatgt
    19561 tgcaaatccg ctccaccgcg agcggacctt cctcgtgcat caccagggcc aagtccttgc
    19621 cactgaggaa ctccatcgcg aggtagagga tcccgtcgtc ggtgcgcccg aagtcgatga
    19681 tgctgacgct attcgggtgg ttcagcctgc tcgaagcgcg cgcctcttga tagaatcgcg
    19741 ccacggtctg ttcatcacca agcaggtgcg gatggatgac cttgaccgcg accgttcggc
    19801 cgagagtcga ttgctcagcc ttgtagactc ggcccattcc accaacgccg accacctctt
    19861 ggagcagata gctcccaccg acgatgcgac ccaccaacgg atcggcggca ccgcccgatt
    19921 cctgctcgtc ggacggagcc ccgcacgcgg aacagaagcg cgcggcagca tctaccgacg
    19981 cgccgcattg gtgacacaag cgcacctctg cagcatatcc taggccgtgc cccgctcaaa
    20041 ctagccgccg ctgaacgcct ggtcggtact agcgctgttc ttcgtcgagc cgccgcatgg
    20101 cttcgagcag caaatggtgt gttggaatcc cgatgacgtc ctcgacagac cggctctccg
    20161 gctcgagctc gaaatccccc tccaccagtc ggaggattcc atacacggcc tcggcccctg
    20221 cgaggtcgcc aagccgcgca tcgtggatcg atccgtcctg aaacatgagc tcgccctgga
    20281 gagtcccgcc cctcaccttg agacgcccgc tctttcgacc gttcgcgaga atttggatca
    20341 cgtccgggag cgacatttct tggagcgagc cggacacgcc acggcctccc ggcttcttga
    20401 cgcggctttc cgagatgatg cggctggcct tggcggccac gaccgcagcg gacgccggct
    20461 tcacgacgta gtcggcagcc ccgagctcga gcccgcgatt caccgaggac tgggccctct
    20521 cggcggtcac gaacatcagc ggaacctgcg cgttcgagcc ttcgcgcata cgccgaagaa
    20581 ggtcgaaccc cgcaccgtcc gcgagccgta cctcactcac gactacgtcg tacttctctc
    20641 tattcaagca gcggagcgcg gcctcgatgg agcgttcgat cgtcacttcg taaccatgtg
    20701 aagcaaactg tacctcgagc acagcagtct cttccgggtc ggggtcgacg atgagtgcgc
    20761 gatgacggtt cgacatgatc tggctcttca taccgtcatc gagaacgatg tgtcgcaacc
    20821 agtcgacgac agccggatcg aagctcaaac ccttgtgcgc ttccagggca tcacaggcgt
    20881 cctgtgccgc taaccgtcgc ccaaatgcgt ttgctgagct ctgggtgagc tcgacgtagc
    20941 tctcggccac ggcgagaacg cgggctccca gggggatgtt cttgccgctc agtcgttcgg
    21001 gaaaaccctg tccatcgaac tgctcaaagc ggtgcctgag cgcatcgacc gccgtctttg
    21061 ggagtgtcac tgactcgaga agacgaagcg gtgccgtgta gattcgtcgc gcggcctcgc
    21121 gattcgactg ttcgtgcgcc acgtcgaaga gcgtgacgtg ggactcggcc gaaacaccca
    21181 aatcgtgtag gtacgcagcg atcaagatcc cttggatctg aggcttggtg agtcgaatcc
    21241 gctcgccaac ctgctggctg acgcgggcaa cgcgcatcga atgtccccca cggtcctggt
    21301 tgagctgctc tatcagcgtg acgaggacgg cgagagtctc cagatacacc tccgggtcga
    21361 cggcgagatc gccaccttgc gagggcgcgg aaagctgctc gatgagcgtt tggccggcag
    21421 agacgcgggt ctgcgtttct ggcggcgctt gttcggcact gggctcattg agctgattgt
    21481 tggccgcttg ctcgatttcg atcgattgcg cccggcggcg gcgaaccaga agcccgtcct
    21541 tgtcctcgaa gacctgaccc cagccgggaa cgggatccga gtcacgtgag gagcgcactt
    21601 gtcgagacga agcaccgctg agcagcattt cgaagggaag attgtcctgg tcgtagtgct
    21661 tggagatcag cgcgcgtatg gcggcctctc gtgcgacata gaccttcacg tcgcgcgcgc
    21721 cggtggcgaa ctgcatttgt tgacgcacat cgtcgtcggt gacgtccgca gcgacgaccg
    21781 acagcgtccg gctcttcaag tcgaacaaga tcggaaaagc gatgagcttc ttggcaagct
    21841 ttcgtgacac cttggcgaga agatcggcct cgacgccggc ctttgcgagc cgctcggagc
    21901 cgacgaaccg ggttcggtag aggccggcga tgtacttgag gagctcggct tcgctcatca
    21961 gtccgagctg aaggatcgcc tcctcggccg tgcactccgt cctctgcgcg tgaagcacga
    22021 tttcgtcata gcgacttggg gacacctgtc cctccacgac gaggcgatcc aaaatcgcaa
    22081 caccacggat tcgttcggcc tgttcttgcg aagccccccg actcacagtc tctacagtct
    22141 agcggaggaa ctcgtggcct gctcaccccg gggacgaaaa gtccaaatcg acaagaaagc
    22201 ccggcctgct agagtctgcc gcagtgaggc ggcggctggg aatctggctc ttcgtggggc
    22261 tgacgacgat gggttgtgcc agcaccggcg ctctccaggg cggcgtattc catggcacgc
    22321 aaacgtccta ccggattgga ccggtcgccc ccgactggac acctgtcacg gtgaaccatc
    22381 agaacgacct cgcctggcac aacgctgcag agggggctgt gatgcatgcg gactccgact
    22441 gtgatcccgg actcgacatc ccgctcgcgg ctcttcgaaa ccatctgata atcggcttca
    22501 cggagcgtga ggtgctcgaa gaagaactgg taccgatgga cggccgggaa gccctgcgca
    22561 cgcacttccg tgcaaagctc gacggcgttc cgcgtgacat gcttttgcag atcctgaaga
    22621 aggacggctg cgtctacgac ttcgctctcg tcacgcctcc ggacgcgagc ttcgatgaag
    22681 ccttgttgga cttcgaccag atgctcgcag gcttcacgac tgcgacgggt cggccgtgac
    22741 ctccgcgtcg ccatccactc ggccgcgtaa cttcaccagc tgggcggatc tccagattcg
    22801 gcgtcttcga aaattcgtcg aagaagtcgg cgacgcgacg ctccttgccg gtcgcacgat
    22861 ccgcaccttg ttcacgactc ggttcgacag cacggaattc atctatcagc tcgagcaact
    22921 tggcgtccgg agcctcgcga tcgctgcggc gacagcgatt ttcgtcggca tcgtcatgac
    22981 catccaattt gccgtgtcga tggaagcgtt tggcgccaag gacaccctgg ggcgggtcat
    23041 cggggtttcc gaagcgcgcg agctcgcacc ctcactcaca gcactggtcg tcgggagccg
    23101 cattgcggcg gggatcgccg ccgagctcgg atcgatgacc gtcaccgagc aggtcgacgc
    23161 gatccgggcc ttgggcgcgg acccgatccg aaagctcgtg gtgccgcgtt tggttgcttg
    23221 catcgtgatc atgccgctca tcacgttcat agcgttcatc ctcgggatga ttagctcgat
    23281 gctggtcgcc gcactttctt acgggattcc gatggagttc ttctcgtcga cggcgctcga
    23341 caccctccga atccaagact tcgtgagtgg ggtctccaag acgcctttct ttggtttcct
    23401 catcgctctt ctcggctgct atttcggctt gcagacccgt ggcggcaccg agggcgtcgg
    23461 ccgctccacg acccgagcgg tcgtggtcgt ctcgatcagc atcttgatgg ccgatgccct
    23521 gctgactcag attttcgtga gcttcggcac gctctgaaga gaatcagatc gaaaccagga
    23581 cgtcggacgt cctagagaac atggcacgct ggctcatcat ctggtcgctt ctcggtgctg
    23641 ccgggtgctc gtcgggcggt ccacagccct tgcgctcgtc gaggcaagac ctcagcccct
    23701 cgttgatgga tgcgccggcc ctcgatctcg gcagattggc gaccactgaa gactgtgccg
    23761 actgtcacag cgatgtcgcc tcccattgga tgcacagcgg acatgcatac gcctcattcg
    23821 acaacccctg gtaccgcgca agtgtcgatc ggtttcgcga gcaacgagga aacgaggaaa
    23881 gccggttctg cgcgggatgc cacgacccgc ttcttctcat gtccggcgac atcgatcagg
    23941 aggtgtcgcc cggcaacgcc ctcgcgtatg caggggtcac ctgcctcgtc tgtcacagca
    24001 tcgagtcgac ccgtccagac gggaacgcga gcttcacgct gagcgacgcc ccggtcttgc
    24061 tccccgatcc ggcgaacccc gaggagatcg aagcacaccg cgcccgactc acgatggcgc
    24121 cgctccggac cgcagggctt tgcggctcgt gccatcggtc gttttccgga ccgatgatcg
    24181 gcaacgaaaa ccacttgcct ggcatcgatg acctcggcga ctgggcctct tccgcctacg
    24241 ccggcgccgt ccaagaccag ctcgtcccgc tcgaagagaa gagttgccag ggttgtcaca
    24301 tgcgccctga agtcgcatcc gaggccgaga tggcgggagc gatcgatggc cgggtgagca
    24361 gtcaccgctg gagtgcatcg cacacggcaa tggccgctca gctaccgggc accaagcacg
    24421 cggagcaagc ggtcgatgag ctcgaaggag cggtcctggt cgacatcggt cccgtgaggg
    24481 caggcgcgcg gcgctacttg ttgccggagc ggtcacgtct tcgcgcgggc gagcgactgc
    24541 tcttcgacgt tttgctccag aaccactcga cgggacaccg atttccgggg ggtgtgcggg
    24601 acatgcacga cgtctggctc gaggttgaag tccgggacgc atcgggtacc ctgctcggcg
    24661 tttcgcagcc gacggagcgc ggaaacgacg acgtcttcat cctccgcgcc accgttctcg
    24721 acgccgaatc gagtccggag atcttgcacc aagtgcaccg tttctctgca cccgcattcg
    24781 accaaacctt gccggcacac gatgcacgtg cggtgcgcta ttcgatgaag ctcccgatgg
    24841 cgctgaacgg tccgatccgg atcgaggcgc gcctcctgca tcgaaagcac agcctcgagt
    24901 tccaggcact ggcttgcgaa gcgagccgca ccgaacgggg cgttgagttc gcccggggcg
    24961 cagagaagca tggcaagatc gcgctcgatc cctgtcagga gcaaccgttg acggagatcg
    25021 gtgccgctgc agtctgggtg ggccggggag cagcgggccg tcaggccgtc ggcggtgccg
    25081 cgcgaccgga ggtcgaacgg ctactcacgc aggcactcgc acttcttcac gccaagcaag
    25141 aagaggtaca cgtggcccag ccgagcatcg atcgcgcgct tcgcttggcg cgcgaaacga
    25201 ggtccccgga gctggaagcg cgagcgctga tcttgcgtgc gcgcctctca tcgatgcagg
    25261 gacgccccga cgaggcggac gagctcttgt cgggcgctcg ggcgttgatc ggaccgagcc
    25321 cggttctgga ccgtgtgcgg ggcgatgcgt acgcgcgcgt gtggcgctgg agggatgcgg
    25381 tggaggccta tcgacgcgtt gccgaatact cgccactcga ctcgggtgcc tggagaaatc
    25441 tcgctcgcgc atacggaagc ctctcagacg acctgaacgc acttgccgcc gccgatctgg
    25501 gacttcgcct cgcgccacgg gacgagagct tactgaggag ccgagcccta gccctcgaaa
    25561 gcctcggtcg accggatgcc gagcaggcga gaaaccgatg gctcgcgcac cgtgccccgg
    25621 atgcgcaggc ccaactcctc gcgacttgtg agcaagaaca ccagcgctgc cgccgagatc
    25681 ggcagccgat accgcactac actctcttac ccccgcgaaa agcaattcac cctggcgccg
    25741 atcccggata agctacgggg aatggcgaag cgatatcaaa agaagagctc caccaacaag
    25801 aagcgcgaaa agaagggcca tggcccatcc cgcaacaccg tgggtggcgg acaaaccact
    25861 ggcgtcatgg gcaacatggt gggtggcttc cggcgagccg tgggttcgga gagctcgaag
    25921 gacagcaagg gcggcagcct tctctggacc gctgttttgg tcaccgccgc aatcgccatc
    25981 gtcgcctgga acttcgcgcg ctagtcgtgg atggctcgcc agcgcagcca atggtccgct
    26041 agaacgagcg cgaccatcgc ttcgcccatg ggaacgaaac gaggcaagag gcacgggtcg
    26101 tgacgtcccg tcgtgcgaat ggtcgtagca tcaccgctgg cggtcacggt gtcttggctc
    26161 cgtggaaggc tgctggtcgg tttgactgca gcgcgaagga gaatcggcat gccgctcgaa
    26221 atcccgccta gcatcccgcc atgtcgattc gaacgtgtgc ggatggttcc ctcggcatcg
    26281 gtttcgaatg cgtcgttgtt ctcactgccg cgggcggtgg cgaccgcgaa tccagcccca
    26341 tactcgacgc ccagaaccgc cggcaggcta aacaaggcct ttccaaggtc cgccttgagc
    26401 ttgtcgaata ccggctcgcc cagcccggcc ggaacgttac gtgcgacgat ttcagcgact
    26461 ccgcctatcg agtcctgatc ttttcgcaca ctctcgatga gcttgaccat ctgcccggct
    26521 gcgaccgggt cagggcaacg aacgatgttg ggctcgccgt ctgggagcca ttcgacctgc
    26581 tcgagtgtga cctttgcagg gaccgt
//
LOCUS       DASRZF010000054        23736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4511973, whole genome shotgun sequence.
ACCESSION   DASRZF010000054 DASRZF010000000
VERSION     DASRZF010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 23736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23736
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4511973"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VFG22_02505"
     CDS             complement(<1..494)
                     /locus_tag="VFG22_02505"
                     /inference="COORDINATES: protein motif:HMM:NF023649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HET6415141.1"
                     /translation="MPVRLLLLSSILTFITAAGCGSDSPSTTVPDEFGRCADFDPLRQ
                     VYWGDTHIHTELSFDANMQGTRTSQDDAYAFARGETIPLQPYTADGSPTRTATIDRPL
                     DFVMLSDHAEFLGTLKVCNDPTSPGYNADQCVEYRAAMQFDATPAEVTFIFVEINGLT
                     IFPP"
     gene            complement(537..1364)
                     /gene="dapF"
                     /locus_tag="VFG22_02510"
     CDS             complement(537..1364)
                     /gene="dapF"
                     /locus_tag="VFG22_02510"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HET6415142.1"
                     /translation="MTLPFYKYEGLGNDFLIVEKDALGGAELRREQAIALCDRHRGVG
                     GDGVLVLDLDAPSMQVINSDGSVPEMCGNGIRCAALHLARRANAAAFEVTIDTLAGPH
                     ACVVVNRPGAESVTVQMAPPSLVPSDLPLSSAAPWLEHALEVNGQTVHLTGVSMGNPH
                     AVTFDPLGDERLSLGPAIQADPHFPEGVNVGFVSEHEGPSMRLDVLERGAGWTEACGT
                     GACAAAVAAVETGRAQPKEKLSIRLPGGTLVITVGAPGEGVLMEGPARFVFRGEVGL"
     gene            complement(1777..5037)
                     /locus_tag="VFG22_02515"
     CDS             complement(1777..5037)
                     /locus_tag="VFG22_02515"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HET6415143.1"
                     /translation="MTSNLEIEGARRAEVSQKSGGLSIERKLTGAGTDPLEEVEYVLR
                     DSQISNSDGSVVFELKGAQVPEGWSQLATDIAVSKYFRKAGINGDKKLGERSVRQLVY
                     RVAHSLREAGDKFGGYFANQEAADTFEAELSHLLIHQKAAFNSPVWFNCGLYQRYGIE
                     GSGGNYAWDPVQDVIFETKTAYENPQCSACFIQAVDDDLMAIYELIKNEARLFKYGSG
                     TGTNFSKIRGKQEKLSGGGTSSGLMSFLEVLDRAAGATKSGGTTRRAAKMVCLDMDHP
                     EIVDFIEWKVREEQKARALIASGYDSDFNGEAYHTVSGQNSNNSLRVTDEFMKAVETD
                     GEWKTTFRTSGEVCDTYRARDIWRKVARAAWGCADPGVQYDTTINDWHTCPNTDRINA
                     SNPCSEYMFLDNSACNLASINLSKFLDDQGHFDIEGYRQAIRILITAMDIAVDYASYP
                     TQEIAKNSHRFRPLGLGYANLGTLLMQLGIPYDSDEGRTICAALTSILCGHAYATSAE
                     IAASKGPFAGFQPNRQPMLRVMGKHREAAYRINEADGPGMTEDEPTACPPELIQAAQE
                     DWDLACKLGELYGYRNSQTTVLAPTGTIGLLMDCDTTGIEPDFALVKFKKLAGGGHFK
                     IVNQSVPNALRRLGYTDAQVADIVSYVTGTNTFTGAPHCGRKALLENGLTEREIEKAE
                     NALRGVFDVGFALAPWVIGTEAYERLGIPAATYNKPGFHLLRFWGHSEKEIGEFNDVV
                     IGRMTVEGAPHLRAEHLPVFDCANRCGKIGERFLEPMSHVHMMAAAQPFLSGAISKTV
                     NLPKESTVEDVEEIYEEGWKLGLKAIALYRDGSKSSQPLNTKQDSEGEEKEEAAEAKA
                     PKVEAPVQEWLTPPKTRRHRLPKKRSGFTQEARVGGHKVFLRTGEYVDGSLGEIFVDM
                     HKEGAAFRSLMNCFAISVSMGLQHGVPLDAYVRQFTFTRFEPQGIVEGHPNIKFSTSI
                     IDYVFRVLGVEYLKQYDFAQVPPKEEQEELQNPTDVAAVQRLEGAQPSEPPPAIKPAE
                     QVTFGFEEGAHQANPLDQQLGEMMGDAPMCDKCGHITVRNGACYRCLNCGNSMGCS"
     gene            5607..6467
                     /locus_tag="VFG22_02520"
     CDS             5607..6467
                     /locus_tag="VFG22_02520"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HET6415144.1"
                     /translation="MRVHLVDGTYELFRAWFGAPEASSPAGREVGATRGFLRSLAAML
                     RTGEVTHVACAFDQTVESFRNELFEGYKTGEGLEPDLLSQFTLAERASQALGIVTWPM
                     VEFEADDALASGAAKFRGAPGVEQVLICSPDKDLTQCVDGTKVVTLDRRRQIVLDEDG
                     VREKFGVSPESIPDYLALVGDAADGIPGIPRWGAKSAARVLAKWRHIEDIPEDHELWG
                     VEVRGAATLAKNLGTRRQDALLYRRLATLRTDVPIDESLNELRWRGPERSALEALCEE
                     IGERTLLDRI"
     gene            complement(6469..6999)
                     /locus_tag="VFG22_02525"
     CDS             complement(6469..6999)
                     /locus_tag="VFG22_02525"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HET6415145.1"
                     /translation="MIYELGERRVRIEGEVYVAPSAAVIGSVVLKDKSSVWFNAVLRG
                     DSDEIAVGEETNIQDGAVLHTDPGIRLILGRGVTVGHHAMVHGCEVGDYSLIGIHAVV
                     LNHAKIGKYCTIGANALVTQGQEIPDYSLVLGSPGKVVRQIDPSDVGSLEKSAAGYVR
                     RAHEYLRTFRADDRWS"
     gene            complement(6996..8162)
                     /locus_tag="VFG22_02530"
     CDS             complement(6996..8162)
                     /locus_tag="VFG22_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415146.1"
                     /translation="MFKTICLCSSIALASFALPRLSFAQSQGWGEPIAAPRKPEPAPA
                     VVEPEPAPSVQTPPETTVAPEKATNPDWSDAPPMEDEARSRDNPDWVDEVEAPPVGRM
                     KDRASEVYGIRYANRNLTMPRGMMRGTFDTVAGRRTENPQGTNEPFGPTGTISSMNFG
                     VAISLAKDFEIGFSRYRMASFPGVSVYPGFGFGDEGLISFSMSPEVEFGDIPLYLRFE
                     ALSSDHVKLGFDAVFRIPVRTEFGFLGGIPMRFVIQEQFAFDTALEFTIDSNPQGPSV
                     WSLNIPVTFVANATDRLFFKLSSGMNLFDLSQTIRTATSGLVNGPFYFIPLGATVGYT
                     VPARSTMMDVFASFRFPALYGFTTRASAVTTETWQVTIGLNIYSPVLFKGRAGL"
     gene            8269..8345
                     /locus_tag="VFG22_02535"
     tRNA            8269..8345
                     /locus_tag="VFG22_02535"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8303..8305,aa:Met,seq:cat)
     gene            8420..9904
                     /locus_tag="VFG22_02540"
     CDS             8420..9904
                     /locus_tag="VFG22_02540"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET6415147.1"
                     /translation="MPVASRRRLATHAGVQAAGLSFVLSVAGWFALRWGGIEVALPLF
                     LWVTAVATAVAGLLTAFYVELSVGRRIARVVRVLDAHARESSLQRLPNLGDDEIGEIG
                     RAVNDLLANLTSLEVRMIEQGQELRHTREELVLKKALGLKSAELEQRLRERALLFDIV
                     RASASEQQLDSVLGDIATRLGKALQLRECMLFLVDAETRRLTLRAAYGFDNPGELLER
                     IVLFDEDALGKVAQLGEPIVIDDLSELEENSIWEPILQRGSIAILPIIHHGKTTGVMA
                     ASRQETNGFSEVETGLLEAISNQLGLAIRHTQLFDELRRGSQHDDLTGLGNRRLLRIR
                     LEDELHRADRFGQAVSVLAIDIDYFKALNDRHGHPTGDASLRKLAGLMTRNLRRIDTI
                     ARIGGEEFVVLLPRTNLTEAAQVADKLRSMVERTEFPGGEGQPDGMLTVSLGVASLRP
                     DETGADLLSRADAALYEAKDRGRNCVVTASQSAGSARSLTRSRI"
     gene            complement(9859..11409)
                     /locus_tag="VFG22_02545"
     CDS             complement(9859..11409)
                     /locus_tag="VFG22_02545"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6415148.1"
                     /translation="MTRLRLRTLVVDDSSSIRALLRELLLERGHEVTTVGSAEEAIKA
                     HAEQPFQLMLIDWTLPGLSGLDLCRMVRGRPGGEDLVILVITGRDRSEDLNAVLDAGA
                     SDYLPKPVDPEILATRVLIAERAIEESARRANEQHDMESRLALADRMASVGTLTAGVA
                     HELNNPLMYVLSNLRLTREEIAELGETEWSERVKQQLDEAILGALRMQDIVRDLKIFS
                     RVDDEPRGNVDVQTVLESSINICWNEIRHKAGLERDFGPTPPVDVNESRLGQVFLNLL
                     INASQAMPDRPVSSNRITVRTHTDSEGWAVVEVADNGTGIEPERMSRVFEPFFTTKAV
                     SEGTGLGLSICRNIVRDAGGIIEVESEPGRGTRFVVKLPASATSHVTQETQAPPIASA
                     GARARVVVVDDEPLVGRSIRRALRDHDVHIYSSGEEAIKRLCSDEPVDVVFCDLMMPE
                     VSGMEVYRRVSEQRPENASRFVFMTGGAFTPEAREFLNETPLFCLEKPFELRQIRDLV
                     RERAEPAD"
     gene            11532..12212
                     /locus_tag="VFG22_02550"
     CDS             11532..12212
                     /locus_tag="VFG22_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415149.1"
                     /translation="MTRSNPLLRTGTRLRRQSTVLVQQTRTASEMFLVESRDASAAFG
                     QELKAATSKWADTTRRSVDGFRDALLKEALDWQKLVLQSRDAYVAAVSERLRRVERQA
                     LSTREALTPEVVETTVLESARGLLQRAQERVDERLEHATKPAPRQKPKKATAAKTRSK
                     PKSKKQLAENGQAPLRNYDQLTARDVVARIQRLSGPQATAVLEYERAQKKRATVIRAI
                     EQRLSAAG"
     gene            12277..13665
                     /locus_tag="VFG22_02555"
     CDS             12277..13665
                     /locus_tag="VFG22_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6415150.1"
                     /translation="MVDTKQVLVADDEDNLRRVLRAQLEHDGYEVHCVGDGEAVFQAL
                     AEHHIDVLITDLRMPKLDGMQVLKLASERFPNIPVIMITAHGTVDTAVEALKLGAFDY
                     VTKPFDRAEFRTVVSKAARTRELSKKHVSGDPNERGRYRIIGESEPMHGVYEVIEKVA
                     DTPSTVLITGESGTGKELIARALHENSSRKNKPFVSVNCAAIPPDLLESELFGYEKGA
                     FTGAVTSKPGRFELADEGTLFLDEIAEIPVSMQVKLLRALQEQEFERVGGIKTIRVDS
                     RLITATNRDLKSSIADGEFREDLYYRLNVVHVHLPPLRDRPSDIPLLLEHFVRKFNAK
                     LGRAVTGFEDAAMSQLLRYDWPGNIRELENVVERCMIFAEDGEVSVRHLPAELGQADR
                     GTGLGTPTAAVPGKTGLKEAVREATLKVEREFIGRALDQTGGNVTHTARLLKISRKSL
                     QNKMKELGLRNE"
     gene            complement(13669..15642)
                     /locus_tag="VFG22_02560"
     CDS             complement(13669..15642)
                     /locus_tag="VFG22_02560"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6415151.1"
                     /translation="MLVKKVQTSAIARTRRIRVLIASRDPERIMLVRESLHAWPETVV
                     CRVALNREDALTQIESHQPHVLCWDSNLGADLPVSARRICTLLLGEAACTAPEEQPWA
                     MPLPDLYVSGLRQSINREYELRRLLIQLRDAHGTETHIRQLMSRATDGIVVLDRAERV
                     LFANAAACAMLHMECAQLLGEPFTHPIPVEGAPTGYQVQLPDGQISALTATSMLSIWD
                     GSEATIIQLQPSQRPSVPPGRLSSVPPGMLDSRVSEDRLRAVGRLAAGLAHEVNNPLT
                     FVLANLESLRESHQAICRFVRDLRVDLATREAITPSSFELLASEANLQEVLDDAADML
                     TDCYKGTHRIQDIARSLGTFSRADDNQAEMVDITRVVDDACAMVFNQIRYRARLVKRF
                     EPIPNIAAFPGRIAQALVNMLTNAAEAIDGGAYEKHRIVVSTRVVGDHIVVGVRDTGA
                     GIQDDDRKRIFTPGFTTKAHEGGMGLGLSACKRVAEEHGGRLEVHHLPDRGTRFELLL
                     PLDTGLQASEPRRESRPASEAPTKRSRLLIVDDDAMVLSALSRRLRRRYDVVTVLGGV
                     EALARLAEDPSFDSIVCDLMMPEIDGKSFYDAIIKDHPELADRIVFMSGGAFTPRLRK
                     FASAVSNPVLQKPVSREELELMLARHRDDTAAE"
     gene            15738..17519
                     /locus_tag="VFG22_02565"
     CDS             15738..17519
                     /locus_tag="VFG22_02565"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET6415152.1"
                     /translation="MLDKRDGEEEHELRDVLLVEDSLIDAQLIRRLLRRVSAAYYRIT
                     HVRTLNDAVLSAEELTPDVILADLNLPDSRGTQTVASLQTSYPDIPLVIVSSWEDEAV
                     SLRSVKAGAQDYLVKGHIDGANLHRVIRYAIERKRTELELVRLAHYDQLTSLPNRTLL
                     RERVDHALARALRAGSGVATLILDMDRFKEINDMLGHEIGDKLLIEAAERIRASVRDQ
                     DTVARLGGDEFAVILEGVSEAKEVLPVIERIIASLSEVTTIDGHEVNSSTSVGIAMYP
                     ENGRDLSELLRAADLAMYQAKSSGRGRYQFFADAMQEEAQSRHALEWALRRAVEKDEF
                     ELVYQPQICLRTGVVIGVEALIRWRSPTRGLLTPYHFISGLEEFGLINEVGEWVLRTA
                     CEQVRRWHAMDFEPMRIGVNVSAQQFEDPMLLDKIRNALKETKIPPSFLELELTESCL
                     MSDPAEAGALLREIREIGVRIAIDDFGTGYSSLTYLNEFPLNALKIDKNFVQGVESND
                     RGGPISNMIIGLGQNLGLEVIAEGVETEAQLMYMREHGCDIAQGYFYAYPESPEKLTD
                     WLKANQRASETYVRSIPIKKIDKETGT"
     gene            17550..18920
                     /locus_tag="VFG22_02570"
     CDS             17550..18920
                     /locus_tag="VFG22_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6415153.1"
                     /translation="MAAATILVVDDERNILSSVSRALALEGYEVEVAGSAEIALQKLA
                     KQTFEAILLDVQLPGMDGLQLLDALREREISTPVIMMSGHATIEVAVEATRRGADDFI
                     EKPIGSERLLLSLKRSLELRELQQENRELRRRYGAGEALLGSSPSMNRLREQIELAAR
                     SNAPVLILGERGTGKELVASAIHAASARAKGPLEKLNCAAVPEGLIESELFGHEAGAF
                     TGATKRRRGKFERAHGGTLFLDEVGDMPPQMQAKLLRVLQEGEVERVGASSVAKVDVR
                     VVAATNKALEAEIEAGRFRADLFDRLNVVPLRVPALRERIEDVPMLTEHFLALACEVH
                     DRPGKALAPGAIGLLKAYPYPGNVRELRNLVERLVILTPDARISEAQARALLPIESES
                     ASGTHFRPETPLREMLDSVERELITKALEFREGHVTKTAADLGLERSHLYKKMRALGV
                     RKPEPE"
     gene            18975..19811
                     /locus_tag="VFG22_02575"
     CDS             18975..19811
                     /locus_tag="VFG22_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET6415154.1"
                     /translation="MFDKLLPIDPEVAARIEALDLQFNKFGVDPYGIDKTDLTRFVSA
                     FAWIYRFYFKVDVFGIENVPSTGRGILVGNHSGGVAIDGAMVMGSMLLDAEPPRLPHA
                     MIDKFIHEFPGASQLMSRTGQFTGNPDQAARLLRAERLILAFPEGARGTAKLAKDADS
                     LVGFGTGFMRLALETKSPIVPFGFVGGGDALPTIANLKRLGRLFGVPYVPVTKWGLLI
                     PKPTRFQLLYGRPMTFEGTGHERDEVVESMVGQVKERIRDLIRQGRQLREKEISEEDL
                     VL"
     gene            19811..20725
                     /locus_tag="VFG22_02580"
     CDS             19811..20725
                     /locus_tag="VFG22_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET6415155.1"
                     /translation="MKVLVTGISGKLGRITALKLLKHGYDVIGIDRRPWPEAPEAIEV
                     FQADIRKRPAEDVFRTHRPDAAIHMATVTHLVHKSPDRMRVNLYGTRAFIHHCHQYGV
                     KQAVFAGRHTFYGAAADSPLYHTEDEPPISAHTFPELADLVAADLYAASALWRYPGIA
                     TAVLRIVYTLGPSKHGTLASFLKGPNVPLVMGFDPLFHFIHEDDAATAIVSALAHKLH
                     GIYNVSGPTPVPLSTIVRAAGRRAIPVPETIFKLGVGRLGLPHLPAGAIDHLKYPIVV
                     DSEAFRRATRYTYEFDERETINSFRTAK"
     gene            20964..21932
                     /locus_tag="VFG22_02585"
     CDS             20964..21932
                     /locus_tag="VFG22_02585"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6415156.1"
                     /translation="MLVSDERSELRGQLLDGRYRIGSAIGMGGTGIVFEAERLATLER
                     VVVKMLRPTHSGNPDLMSRVRREVEVARRVFHPGIVPVIDHGALYDGSPYLVMKHMRS
                     ESLASYLRRHGPLNGPQISVIMARLASILHSVHRHGYVHRDIKPEHILLNRGGYGELD
                     VHLLDFGVCAADTAPPDERARERGRVYGTPSYASPEQASGNPFVDARADLFAVGITMF
                     EALTGKLPFTGADVTAVLRRIIREDAPRARHLSPYVDDEMDGLVAKLLSRSPAARHPS
                     ARALMRALEPWLVERVRTERALASTLQVAPDTSSIRPTAQHEMVAA"
     gene            complement(21929..22849)
                     /locus_tag="VFG22_02590"
     CDS             complement(21929..22849)
                     /locus_tag="VFG22_02590"
                     /inference="COORDINATES: protein motif:HMM:NF004957.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase"
                     /protein_id="HET6415157.1"
                     /translation="MLPIDLSGKRAFVAGVADDAGFGFAITKALAEAGATVCVGTWPP
                     ALGVFETLLRRGKLDASLQMSDGSTLELERIYPLDAEFDSLEDAPEDLRSQKRYRDRG
                     DFSIDGLANRLREDFGGAALDIVVHSLANGPEVNKPLMQTSRRGYLAAHGASAFSFVS
                     LAQRLAPLMRENGAFLGLTYLASQGVVSDYGGGMASAKAALEADARMLAFELGRKHGH
                     RVNLISAGPHASRAASAIGFIHKMIKYYEMNAPLPGALRAEEVGHAAAFLCSPLASGI
                     TGTVLHVDKGYHAMAAPADVGALIDRLAGN"
     gene            22897..>23736
                     /locus_tag="VFG22_02595"
     CDS             22897..>23736
                     /locus_tag="VFG22_02595"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET6415158.1"
                     /translation="MKKERRGVEYGGVDESYFEDRGLRRYAGVWSLWALGVGAVISGD
                     FFGWNFGLASGGFGGLLIATVIITIMYAGLCFSIAEMAAALPHTGGAYSFARSAMGPW
                     GAYITGLAENMEYILTPAVIVVGIGGYLGAIFGTPASVEPLWWLASYAVFVGLNVAGV
                     ETTFRFTVLITAMALGVLLVFWIAAIPHFDMSWALDIEPEPGHDRWLPKGIGGVLSAL
                     PFAFWFFLAIEQLPLAAEEAQAPARDLPRGLLYGLLTLIVAAFLTLFLNAGIAPGAEV
                     LGSS"
BASE COUNT         4532 a         7347 c         7444 g         4413 t
ORIGIN      
        1 tcagggggga agatcgtgag cccgttgatc tcgacgaaga tgaacgtgac ctcggccggc
       61 gtcgcgtcaa actgcatcgc tgcgcgatac tcgacgcatt gatcggcgtt gtagccgggc
      121 gacgtgggat cattgcaaac cttcagcgtc ccgaggaact cggcgtgatc gctcaacatg
      181 acgaagtcca agggtcggtc gatcgtggcg gtccgagtcg gagagccgtc cgcggtgtaa
      241 ggctgaagcg ggatcgtctc gccgcgtgcg aaggcgtagg cgtcgtcctg cgacgttcgc
      301 gtgccctgca tgttggcgtc gaacgaaagc tcggtgtgaa tgtgcgtgtc gccccagtag
      361 acctggcgga gcgggtcgaa atccgcacag cgcccgaact cgtcgggaac ggtagtcgaa
      421 ggcgaatcgc tgccgcagcc cgcggcagtg atgaacgtca agatgctcga gagaagcagt
      481 aagcggacgg gcatcgggtg aagatagcgc gggtcggtga tgcggcaatc gaccgtttag
      541 agcccgacct cacctcgaaa aacgaaacgt gcgggaccct ccatgagaac gccctcgccc
      601 ggagcaccga cggtgatgac cagggtgcca cccggcagac ggatgctgag cttctccttg
      661 ggctgcgcgc ggcccgtttc aacggccgca accgctgcgg cgcaggcgcc ggtgccacag
      721 gcctcagtcc atcccgctcc ccgctcgagg acatcgagtc gcatgctggg gccctcgtgc
      781 tcggagacga aacccacgtt gacgccttct ggaaagtgcg gatccgcttg aatcgccggc
      841 ccgagggaaa gacgctcatc accgagcgga tcgaatgtca ccgcatgtgg attacccatc
      901 gaaacgcccg tgagatggac cgtctgtccg ttcacctcca gagcatgctc gagccaaggc
      961 gccgccgagc tcagcgggag gtcggaggga accagtgacg gaggcgccat ctgaacggtc
     1021 accgattcag cgccgggccg attgacgacg acgcaagcat gaggcccggc cagggtgtcg
     1081 attgtcacct cgaaagccgc cgcgtttgca cgcctcgcca ggtgcaaggc cgcgcagcga
     1141 atcccgttgc cgcacatctc tggcaccgag ccgtcggagt tgatcacctg catcgaaggc
     1201 gcatcgaggt cgagcacgag cacaccgtcc cccccgacgc cgcgatgccg atcgcaaagc
     1261 gcgatcgcct gctcccgcct gagctcggct ccgcccagcg cgtccttctc gacgatgaga
     1321 aagtcgttcc cgagcccctc gtacttgtag aacggcagag tcactgagaa ggagttctaa
     1381 ggctcggagc aaaagaccgc aaaacgcgga gggcacgagg ccccggcact gacgccggca
     1441 ctgacgctgg cactgacgct ggcactgaca ctgacgctgg cactggcact ggcactggca
     1501 ctcgcgctgg cactggcact ggcactggca ctggcactgg cactggcact gcctggccca
     1561 aaaactccat aattacaatg ccccagtgca taccccgccc tgacggggtc tgagtcgcgt
     1621 cgatcggcga gcgcccccgc ccagtaccga ggtccctggc ggggggagcc gtcgtcagct
     1681 gccgagcgcg cccggagaac cgtagcgagg aatactgagg tattccgcag cgaaggttcg
     1741 aggacgtaag ccggcagatg gcggcggatc gccccgtcag gagcagccca tggagttgcc
     1801 gcagtttagg cagcgataac acgccccatt gcgaaccgtg atgtgtccgc acttatcgca
     1861 catcggcgcg tcgcccatca tttcgccgag ctgctggtcg agtgggttcg cttgatgcgc
     1921 gccctcctcg aaaccgaagg tcacctgctc ggctggcttg atggcgggag gcggctcgct
     1981 tggctgtgca ccctccaagc gttgcaccgc agcgacgtcg gtcgggttct gaagctcttc
     2041 ctgttcctct tttggaggga cttgcgcgaa gtcgtattgc ttgaggtact cgacaccaag
     2101 cacgcggaag acgtagtcga tgatcgaagt cgagaacttg atgttgggat gaccttcgac
     2161 gatgccctgc ggctcgaaac gcgtgaaggt gaactgccgg acgtacgcgt cgagcggaac
     2221 gccatgctgt agccccatcg aaacgctgat cgcgaagcag ttcatcagcg aacggaacgc
     2281 tgcgccttcc ttgtgcatgt cgacgaagat ctcgccgagc gagccgtcta cgtattcgcc
     2341 ggtacggagg aagaccttgt gcccaccgac gcgtgcctct tgcgtgaaac cgctgcgctt
     2401 cttcggaagc cggtggcggc gggtcttcgg aggcgtcagc cactcctgca ccggagcctc
     2461 gactttcggt gccttggctt cagccgcctc ttccttctcc tcgccctcgg agtcttgctt
     2521 ggtgttgagc ggctgcgagg acttcgagcc atcccggtag agcgcgatcg ccttgagacc
     2581 cagcttccag ccctcttcgt aaatctcctc gacgtcctct acggtcgatt ccttgggaag
     2641 gttgacggtc ttcgaaatgg cgcccgagag gaacggctgg gcagcggcca tcatgtgcac
     2701 gtgggacatc ggctcgagaa aacgctctcc gatctttccg cagcggttcg cgcagtcgaa
     2761 gaccggaaga tgctcggctc tgaggtgcgg tgcgccttcg accgtcatac ggccgattac
     2821 gacgtcgttg aactcgccga tctctttttc gctgtggccc cagaagcgga gcaggtggaa
     2881 accgggcttg ttgtacgtcg ccgcggggat gcccagccgc tcgtaggcct cggtcccgat
     2941 cacccagggc gccaaggcaa aaccgacgtc gaagacgcca cgaagcgcgt tctccgcctt
     3001 ttcgatctcc cgctcggtga ggccgttctc caacaaggcc ttgcgaccgc aatgcggtgc
     3061 gccggtgaag gtgttggtgc cggtcacgta cgaaacgatg tcggcgactt gcgcatcggt
     3121 gtagcccaat cgacggagcg cattgggcac gctctggttg acgatcttga aatggccgcc
     3181 accggcgagc ttcttgaact tcacgagcgc aaagtccggc tcgatacccg tagtatcgca
     3241 gtccatcaag agcccgatgg ttccggtcgg cgcgagaacc gtggtttgcg agttgcgata
     3301 gccatagagc tcaccgagct tgcaggccaa atcccaatcc tcctgcgcag cctgaatgag
     3361 ctcgggcgga caggcggtcg gctcgtcttc ggtcatgccc ggaccgtccg cctcgttgat
     3421 gcggtaggcc gcttcgcggt gcttgcccat gacgcgaagc atcggttgcc ggttgggctg
     3481 gaaacccgca aacggtccct tgctcgctgc gatctcggcc gaggtcgcgt aggcgtgacc
     3541 gcagagaatc gaagtgagcg ctgcacagat cgtccggccc tcgtcgctgt cgtagggaat
     3601 cccgagctgc atgagcaggg tgccgaggtt ggcgtagccg aggccgaggg gcctgaacct
     3661 atgactgttc ttggcgatct cctgggttgg gtagctcgcg tagtccaccg cgatgtccat
     3721 ggcggtgatc aagatgcgaa tcgcctgacg gtagccttcg atgtcgaaat ggccctgatc
     3781 atcgaggaac ttcgagaggt tgatcgaagc cagattacag gccgagttgt cgaggaacat
     3841 gtactccgag cacgggttcg acgcgttgat gcggtcggtg ttcgggcagg tgtgccagtc
     3901 gttgatcgtc gtgtcgtact gaacaccagg atctgcgcat ccccatgcag cccgtgcgac
     3961 tttgcgccag atgtcacgtg cgcgatatgt atcgcaaacc tcgcccgaag ttcgaaacgt
     4021 agtcttccat tcaccgtcgg tctcgaccgc cttcatgaac tcgtcggtca cgcgaagcga
     4081 gttgttcgag ttctggccag agaccgtgtg gtacgcctcg ccgttgaagt cggaatcgta
     4141 accggacgcg atcaaagcgc gggccttctg ttcttcgcga accttccact cgatgaagtc
     4201 gacgatctcc gggtgatcca tgtcgaggca gaccattttg gcagcccggc gagtggttcc
     4261 accggacttg gtggcacctg ccgcacgatc gagcacttcg aggaagctca tgaggccact
     4321 cgacgtaccg ccgcccgaga gcttctcttg cttgccgcgt atcttcgaga agttggtgcc
     4381 ggtgccggag ccgtacttga agagacgcgc ctcgttcttg atcagctcat agatcgccat
     4441 gaggtcgtca tcgacggcct ggatgaagca ggcggagcac tgtgggttct cgtaggcggt
     4501 tttggtttcg aagatgacat cttgcacggg atcccaggcg tagttgccgc ccgatccctc
     4561 gatgccatat cgctgataga gaccgcagtt gaaccagacc ggagagttga aagccgcctt
     4621 ctggtgaatg agcaggtggg aaagctcggc ttcgaaggtg tccgccgctt cttggttggc
     4681 gaagtaacca ccgaacttgt cgccagcctc gcgaagcgag tgggcgacac gatagaccag
     4741 ctgacgaacg ctgcgctcgc caagcttttt gtcaccgttg atacctgctt tgcggaaata
     4801 ctttgaaacc gcgatgtcgg ttgcgagctg gctccagcct tcaggtacct gtgcaccttt
     4861 gagctcgaac accaccgagc catccgaatt ggagatctgg ctgtcgcgaa gaacgtattc
     4921 gacctcctcg aggggatctg tacccgcgcc ggtcagctta cgctctatcg aaaggccgcc
     4981 ggatttctgc gaaacctcgg cccttcgggc accttcgatc tccaaattgc tagtcattcc
     5041 cctcgtgtcc aatgccacag catacctcca accagacgca actcaccatc caggaaaacc
     5101 ccacctttca tccccaggcg gagcacccac tttccacaat cgtcccacaa ggggttgcgc
     5161 cagcagcctc cttgccacca caagcgcttg ggggcgagcc tgcgaagtag ctccgtggca
     5221 aggggccagt caagcagaaa cgatcgggcc caagtgcttt cttattttcc cttgcaattt
     5281 ctgccatttg agcgctccgg gctgctggga tttctgcgat ttcgcagcgc gtcataggag
     5341 cgctgcggtc gaatcacatg agctgacacc tcatgtatgg tcactatttg tatagtacta
     5401 gatgttgggg aaggcgctca aaaaaaccgc aacatttagc ggtgacatgt gcgttgctgt
     5461 gcaacgatga acgtgcctat accaccccgt gttggccgat tccggccttc ggtgaggccc
     5521 tcaagatcgt tctacaagtc cgcgtaatgc gagtccttct tcgcagcgca agatgccggg
     5581 gcaatgatct acggtaagaa cggctcatga gagtgcatct ggtcgacgga acctacgagc
     5641 ttttcagggc ctggttcggc gctccggaag cgagctcacc tgcgggccgt gaggtgggcg
     5701 caacacgggg cttcttgcgt tcgcttgccg cgatgcttcg cacgggcgag gtgacgcacg
     5761 tcgcctgcgc cttcgatcag accgtcgagt cgttccgcaa cgagctcttc gagggttaca
     5821 agaccggaga gggcctggaa ccggatctcc tcagtcaatt taccctcgcc gaacgcgcca
     5881 gccaggcgct ggggatcgtc acctggccca tggtggagtt tgaagcggac gatgcgctcg
     5941 catcgggtgc cgcgaaattt cgtggcgcgc ctggcgtcga gcaggtgctg atctgctcgc
     6001 cggacaaaga cctcacgcaa tgcgtcgacg gaacgaaggt cgtcaccctc gatcgccggc
     6061 gccagattgt cctcgacgaa gacggcgtgc gagagaagtt cggcgtctca cccgaatcga
     6121 tccctgatta cttggcgctc gttggcgacg cggccgatgg cattcccggc atcccgcgct
     6181 ggggagccaa gagcgccgcg cgcgtgctcg cgaagtggcg gcacatcgag gacatccccg
     6241 aagaccacga gctttggggt gtcgaggttc gcggtgccgc gacgctggca aagaacctcg
     6301 gcactcggcg ccaagacgca ttgctctatc gaaggctggc gaccttgcgc acggatgtgc
     6361 cgatcgacga gtcgttgaac gagcttcgat ggagagggcc ggaacgttct gccttggaag
     6421 ctttgtgcga agagatcggc gagcgaacgc tgctagaccg tatctgaatc aagaccagcg
     6481 atcgtcggcg cgaaaggttc tcaagtactc gtgcgcgcgc ctcacgtagc ccgctgcgct
     6541 cttctcgaga ctcccgacat cgctcggatc gatctgccga accactttgc ccggagatcc
     6601 caagaccaag gaatagtcgg ggatttcctg accttgggtg accaaggcat tggcgccgat
     6661 cgtgcagtac ttaccgatct tcgcgtggtt gaggacgacc gcatggatgc cgatcagact
     6721 gtagtcgccc acctcgcaac cgtgcaccat cgcgtggtgg cccacggtca cgccgcgccc
     6781 gaggatcaga cggatgccgg ggtcggtgtg gaggacggcc ccatcctgaa tgttggtctc
     6841 ttcgccgacg gcgatctcgt ccgaatcgcc gcgcagcacc gcgttgaacc agacgctcga
     6901 cttgtccttg agcacgacgg aaccgatcac ggctgccgac ggagccacgt agacctcccc
     6961 ttcgatccgc acccgacgct ctccgagctc gtagatcaca accccgctct acccttgaac
     7021 aagactggcg agtagatgtt gagtccgatg gtcacctgcc aagtctctgt ggtgacggcg
     7081 gacgctcggg tggtgaagcc gtagagcgca gggaagcgaa agctggcgaa gacgtccatc
     7141 atggtgctcc gggctgggac cgtgtagccg accgtggcgc cgagcgggat gaagtagaaa
     7201 ggaccgttga ccagtccgga cgtagccgtc cgaatcgttt ggctgaggtc gaagagattc
     7261 atgccgctgc tgagcttgaa gaagagtcgg tcggtggcgt tggcgacgaa agtgaccggg
     7321 atgttgagcg accagaccga cggaccttgc ggattgctgt cgatggtgaa ctcgagtgcg
     7381 gtatcgaatg cgaactgctc ctggatgacg aagcgcatcg ggatcccgcc taggaagccg
     7441 aactccgtgc ggacgggaat ccggaagacc gcatcgaacc cgagctttac gtggtcgctc
     7501 gagagcgctt cgaagcgaag atagagtggg atgtcgccga actcaacctc gggcgacatg
     7561 ctgaacgaga tcagaccctc gtcgccgaac ccaaaaccgg gatacacgct cacgccggga
     7621 aaggaggcca ttcggtaccg cgagaagccg atctcgaagt cctttgccag gctgattgcc
     7681 acgccgaagt tcatgctcga gatagtgccc gtcggtccaa acggctcgtt ggtgccctgc
     7741 gggttctcgg tccggcgtcc ggcgaccgta tcgaaggtgc cgcgcatcat gccccggggc
     7801 atggtgaggt ttcgattggc atagcggatg ccgtagacct cggaggcgcg atccttcatc
     7861 ctgccgacgg gtggcgcctc gacctcatcg acccagtcgg ggttgtccct tgaacgtgcc
     7921 tcgtcctcca ttggcggcgc gtccgaccag tccggattgg ttgctttctc gggtgctaca
     7981 gtcgtttccg gcggggtctg taccgaaggc gccggctctg gctcgacgac cgcaggcgcc
     8041 ggttcaggct ttcggggggc tgcaatcggc tcaccccagc cctgagactg cgcgaatgag
     8101 agtctcggaa gcgcaaacga agcaagggcg atgctggagc agaggcagat ggttttgaac
     8161 acgatgcggg caataaacca atctcggaca gctgccaagg ccggaggttc acccccgcgg
     8221 acatccacat tgcgcggtag tcccccccgc ctatactcgc gccgctctgg cgatgtagct
     8281 caggtggtta gagcgggcgt ttcatacgcg ctaggtcggt ggttcgactc cacccatcgc
     8341 caccaacttg gacgagcctt cggctcgatg cgcaatgcgg ctcacttgac gaagcgcccg
     8401 cactgcgtag cgtaaaccta tgcccgttgc gtcacgacgc cgactcgcta cccatgctgg
     8461 cgtgcaggcc gcaggcctgt ccttcgttct gtcggtggcg ggctggttcg cgttgcgctg
     8521 gggcgggatc gaagtcgccc tcccgctgtt cctatgggtg accgccgtgg cgaccgctgt
     8581 ggccgggctc ctcacggcct tctacgtgga gttgtcggtt ggacgacgca tcgcgagagt
     8641 ggtgcgtgtg ctcgacgcgc acgcacgcga gagcagtctg cagcgcttgc ccaatttggg
     8701 cgacgacgag atcggcgaga ttggccgagc ggtcaacgac ttgctcgcca atctcacttc
     8761 gctcgaggtg cgcatgatcg agcaagggca ggagcttcgg cacacgcgtg aagagctggt
     8821 cttgaagaag gcactcggct tgaagtcggc cgagctggaa caacgcctca gagaacgcgc
     8881 cctgctcttc gacatcgtcc gcgccagcgc atccgaacaa caactcgact ccgttctcgg
     8941 agacattgca acgcgtctcg gaaaggcact ccaacttcgg gagtgcatgt tgttcctcgt
     9001 cgatgcagaa acccgtcgcc tgaccctccg cgccgcctat ggcttcgata atccgggcga
     9061 gctcctggag cgcatcgttc tcttcgacga ggacgcgctc ggcaaggtcg cgcagctcgg
     9121 agaaccgatc gtaatcgacg atctcagcga gctcgaggag aactcgattt gggagccgat
     9181 cttgcagagg ggctcgatcg cgattcttcc catcattcac cacggaaaga ccacgggcgt
     9241 catggcagcg tcgcgccagg aaaccaatgg attttcggaa gtcgagacgg ggctgctcga
     9301 ggctatttca aaccaactcg ggctcgcgat tcggcacacg cagctcttcg atgagctgcg
     9361 acgcggctcg cagcacgatg acctcaccgg gctcggcaac cgccgactac tccgcatccg
     9421 cctcgaggac gagcttcatc gtgcagatcg attcggccag gcggtgagcg tcctcgccat
     9481 cgacatcgac tacttcaaag cgctcaacga ccgacacggc cacccgaccg gggacgcctc
     9541 ccttcgaaag ctcgcaggac tcatgactcg caacctgcga cgcatcgaca cgatcgcacg
     9601 catcggcggc gaggagttcg tcgtactcct accacgcacg aatctcaccg aggccgcgca
     9661 ggtggcggac aagttgcgct cgatggtgga gcggaccgag tttcccggcg gtgagggtca
     9721 gcccgacggg atgctgacgg tgagcctggg cgtggcctcg cttcgccccg acgaaacggg
     9781 ggccgacttg ctttcgcggg ccgacgcggc gctttacgaa gcgaaagacc ggggccgaaa
     9841 ctgcgtggtc acggcctctc aatctgccgg ttcagcacgc tccctcacca ggtcacgaat
     9901 ctgacgaagc tcgaagggct tctccaagca gaacagtggc gtttcgttga ggaattccct
     9961 ggcctccggg gtgaatgccc cgcccgtcat gaacacgaat cgcgaggcgt tctcgggccg
    10021 ttgctcggac acccgcctat agacttccat gccgctgacc tccggcatca tgaggtcgca
    10081 gaacacgacg tcgacgggct cgtccgagca gagacgctta atcgcttctt ctccactcga
    10141 atagatatgt acgtcgtgat cgcgaagcgc acgccggatc gagcggccga ccagaggctc
    10201 gtcatcgacc acgaccacgc gtgcccgggc accagcgcta gcgatcggcg gcgcttgggt
    10261 ctcctgcgtg acgtgactcg tcgcggacgc agggagcttg accacgaatc gagtgccccg
    10321 ccctggctcg ctctccactt cgatgattcc accggcatcg cgcacgatgt ttcggcagat
    10381 cgatagaccg agaccggtgc cctccgaaac ggccttggtc gtgaagaaag gctcgaagac
    10441 gcggctcatt cgctccggct cgatcccagt cccgttgtct gcaacctcga ccacggccca
    10501 gccttccgag tcggtgtggg ttcgcacggt aatgcgattg gagctcaccg gtcgatcggg
    10561 catggcctgg gaggcgttga tgagcaggtt gagaaacacc tgtccaagcc tcgattcgtt
    10621 cacatcgacc ggcggggtcg gaccgaaatc gcgttccaga ccggccttgt gccggatctc
    10681 attccaacaa atgttgatcg aggattcgag gacggtctgc acatcgacgt tgccgcgtgg
    10741 ctcgtcgtcg acgcgcgaga agatcttgag gtcacgcacg atgtcttgca tgcggagggc
    10801 gccgagaatc gcttcgtcca actgctgctt gacccgctcg ctccactcgg tttccccgag
    10861 ctccgcgatt tcttcccgcg tgagccggag attggacaac acgtacatca aggggttgtt
    10921 gagctcgtgg gccacgccgg ctgtgagggt gcctacggac gccatacgat cggcgagggc
    10981 aaggcgtgat tccatgtcgt gctgttcgtt tgcgcggcgg gcggactcct cgatcgcccg
    11041 ttcggcgatc aggacccgag tcgcgaggat ttcgggatcg acgggcttgg gcagatagtc
    11101 cgaggcgccc gcgtcgagca ccgcgttgag gtcctcgctg cggtcgcggc ccgtgatgac
    11161 gaggatcacc agatcttctc cgccggggcg cccgcgcacc atccggcata ggtcgagccc
    11221 ggagagtccc ggaagggtcc agtcgatgag catgagttgg aaaggctgct cggcgtgggc
    11281 cttgatggcc tcctcggcgc tgcctaccgt ggtcacttcg tgacctcgct cgagcaggag
    11341 ctcacgcagg agggctcgaa tggagctcga gtcgtcgacg accagtgttc gcaagcgcag
    11401 tcttgtcact agcccgaaag tctagtgcag atgctgaatt ggttcaggtt tcgtgtcggc
    11461 cttgacgccc ggtcattcgt agctagcatg tggttggttg acgcaatgcg ttattccagg
    11521 agggtagaca gatgactcgc agcaatccac ttctccggac cggaacccgg ctccgaaggc
    11581 agagcacggt cttggttcag caaacacgca ctgcgagcga gatgtttctc gtcgaatcgc
    11641 gggacgcaag cgccgcattc ggccaggagc tgaaggccgc gaccagcaag tgggccgaca
    11701 ctacccggcg ttcggtcgac ggatttcgag atgcgttgct gaaggaagcg ctcgattggc
    11761 agaagctggt ccttcagagc cgagatgcct atgtggcggc ggtctcggaa cgacttcgcc
    11821 gggttgagcg acaagccctc agcactcggg aggcgctcac gcccgaagtg gtggagacca
    11881 ccgttctcga gtcggcgcgc ggtctgcttc aacgggcgca ggaacgagta gacgagaggc
    11941 tcgagcacgc cacgaagccg gcccctcggc aaaagccgaa gaaagcgacc gccgccaaaa
    12001 cccgaagcaa gccgaaatca aaaaagcagc ttgccgagaa cggccaggcc ccgctacgca
    12061 actacgatca actcacggcc agggatgtgg tggctcgcat ccagcgcctt tccggccctc
    12121 aggccacggc agttctcgag tatgagcggg cgcagaagaa gcgcgcaacg gtgattcggg
    12181 ccatcgagca gcgcttgagc gccgcgggct gagcagactg accgagcctg cccaggtctt
    12241 ctgtactccc gggcaggctg cactacactg cccgccgtgg tcgataccaa acaggtcctc
    12301 gttgccgatg acgaggacaa tctgcggcgg gtcctccggg cgcaactgga gcacgatggg
    12361 tacgaggtcc actgcgtggg tgacggcgag gcggtgtttc aggctctcgc cgaacatcac
    12421 atcgatgttc tgatcacgga cctgcgcatg ccgaagctcg acggcatgca agttctcaag
    12481 ctcgcgagcg agcgttttcc aaacatcccg gtgatcatga tcacggcgca tggaaccgtc
    12541 gacaccgctg tcgaggcgct gaagctgggc gcctttgact acgtgaccaa gcccttcgat
    12601 cgggcggagt tccgaacggt ggtgagcaag gccgcgcgta cgcgggagct ctcgaagaag
    12661 cacgtgagcg gagatccaaa cgaacgcggc cgctaccgga tcatcgggga atcggaaccg
    12721 atgcacggcg tttacgaagt gatcgagaag gtcgcggaca cgcccagcac ggtcctcatc
    12781 accggcgaga gtggcacggg caaggagctc atcgcgcggg cgctgcacga gaacagctcg
    12841 cggaagaaca agcccttcgt gagcgtcaac tgcgcggcaa ttccgcccga cctgctcgaa
    12901 agcgagctgt tcggatacga aaagggcgca ttcaccggcg cggtgaccag caagccgggt
    12961 cgcttcgagc tggccgacga gggcacgctt ttcctcgacg aaatcgccga aattccagtc
    13021 agcatgcagg tgaaactgct ccgagcgctg caagagcagg agttcgagcg cgtcggcgga
    13081 atcaagacaa tcagggtcga tagccgcttg atcacggcaa cgaatcgcga tctgaagtcg
    13141 agcatcgccg atggggagtt tcgcgaagat ctctactacc gcttgaacgt cgttcacgtg
    13201 cacctgccgc cgctgcgcga ccgcccgagc gacatcccgc tgctgctcga acacttcgtc
    13261 cgtaaattca acgcgaagct cggtcgcgcg gtgactggtt tcgaggacgc ggcgatgagc
    13321 caattgcttc gatatgactg gccgggaaac atccgtgagc tcgagaacgt cgtcgagcgc
    13381 tgcatgattt tcgctgaaga cggcgaggtg agtgtccggc acctgcctgc cgagctcgga
    13441 caggccgatc ggggcactgg cctcggaacc cccaccgcgg ccgttcctgg aaagaccggc
    13501 ctcaaggagg ccgtcagaga ggcgaccctc aaagtcgagc gggagttcat cggacgggcg
    13561 ctcgaccaga cgggcgggaa cgtcacccac acggcgcggc tgctcaagat ctcgcgcaag
    13621 agcctgcaga acaagatgaa agagctcggg ctcaggaacg agtgagcgtc attccgctgc
    13681 ggtgtcgtcg cgatgacgcg cgagcatcaa ctctagctct tcgcgactca ccggcttctg
    13741 caagaccgga ttcgaaacgg ccgacgcgaa ttttcgaaga cgaggcgtga acgcgccccc
    13801 actcatgaac acgattcgat cggcgagctc cgggtgatcc ttgatgatgg cgtcgtagaa
    13861 cgacttaccg tcgatctcgg gcatcatcag atcgcaaacg atcgaatcga agctcggatc
    13921 ctcggcaagc cgtgcgagag cctccacgcc tccgaggact gtgacgacat cgtatcgtct
    13981 gcgaaggcgc cgactgagag ccgatagcac catcgcgtcg tcatccacga tcaggagacg
    14041 cgatcgcttg gtcggagcct ccgacgccgg acggctctcg cgccgtggct cgctggcctg
    14101 cagtcccgtg tcgagcggca gaagcagctc gaatcgagtg ccacggtccg gaagatgatg
    14161 gacctccagc cgcccgccgt gctcctctgc gacgcgcttg cacgcgctga gccccagacc
    14221 cattccccct tcgtgtgctt tcgtggtgaa gccgggcgtg aagattcgct tgcgatcgtc
    14281 atcctgaatg cctgcaccgg tgtctcgaac tccgaccacg atgtggtcgc ctaccacccg
    14341 cgtcgacacg acgatgcgat gcttctcata cgcaccgccg tcgatcgctt cggccgcatt
    14401 cgtgagcatg ttgaccagag cctgcgcaat gcgccccggg aatgcagcga tgttgggaat
    14461 cggttcgaag cgctttacta ggcgcgcccg gtagcgaatc tggttgaaga ccatcgcaca
    14521 tgcgtcatcg acgacgcggg tgatgtcgac catttcggcc tggttatcat ccgctctcga
    14581 gaacgtgccg agacttctag cgatgtcctg gatccggtgc gtgcctttgt agcagtccgt
    14641 gagcatgtca gcggcgtcgt cgaggacctc ctgcaagttc gcctcggacg ctagcagttc
    14701 gaacgaactc ggggtgatcg cctcacgtgt cgccagatcg acccgtagat cgcgtacgaa
    14761 tcgacagatg gcctgatgcg attcgcggag gctttcgagg ttggcaagca cgaaggtaag
    14821 cgggttgttc acctcgtgag cgagtcctgc tgcgagacgg cccactgcgc gaaggcgatc
    14881 ctcggagacc cgcgagtcga gcatacccgg aggaacgctc gagagtctgc caggcggaac
    14941 gctcggtctc tgcgagggct gcagctggat gatcgtcgct tcgctcccgt cccagatgga
    15001 aagcatcgat gtggccgtga gtgcagaaat ctgcccgtcg ggaagctgaa cttgatagcc
    15061 ggtgggtgca ccttctactg gaatggggtg tgtgaaaggc tcccccagaa gttgtgcgca
    15121 ttccatgtgg agcatcgcgc aggccgctgc attcgcgaag agcacgcgtt cggcgcggtc
    15181 gaggaccacg atgccgtcgg tagcgcgcga catgagctgc cggatgtggg tttcggtgcc
    15241 gtgcgcatca cgaagctgga tcaagagtcg acgaagctcg tactcacggt tgatcgactg
    15301 ccgaaggcca gagacgtaca gatcagggag cggcattgcc cagggctgct cttcgggtgc
    15361 cgtgcatgcg gcctcgccta gcagcagagt gcagatccgc cgggccgaca ccggcaggtc
    15421 ggcgccgagg ttgctgtccc aacaaagcac gtgaggctga tggctttcga tttgggtgag
    15481 cgcgtcctcg cggttcagcg caacgcggca gacgacggtc tcgggccatg catggaggct
    15541 ttcgcgaacc agcatgattc gctcgggatc tctggaggcg atcaagaccc gaatccgcct
    15601 ggtcctcgcg atcgctgatg tttgtacctt cttgaccagc acgataaccc tcagggctgg
    15661 aatcagcctt gactctcctc caaaaccata gcactgtaat aaggcgctag taacggtatt
    15721 tttaatggtg atggtggatg ctcgataagc gcgacggcga ggaagagcac gagctgagag
    15781 atgtcctctt ggtcgaggat agcctcatcg atgcccagct gatccgccgg ctgcttcgaa
    15841 gggtgagcgc cgcctactac cgaattacgc acgtccggac gctcaacgac gcggtgctgt
    15901 cggccgagga gctcacgccc gacgtcatcc tggctgacct caacctaccc gactcgcggg
    15961 gaacccagac ggtggccagc ctgcaaacct cgtatcccga cattccgctg gtgatcgtca
    16021 gctcatggga ggacgaggcg gtgtcgcttc gttcggtcaa ggcgggcgcg caggactacc
    16081 tagtcaaggg acacatcgac ggcgcgaatc tccaccgtgt gatccgctat gcgatcgagc
    16141 gaaagcgaac cgagctcgag ctcgtgcgac tggcgcacta cgaccaactg acctcgctcc
    16201 cgaatcgcac gctgctgcgc gagcgcgtgg accatgcctt ggcccgagcg ctgcgcgcgg
    16261 gctctggggt ggcaacgctc atcttggaca tggatcgttt caaggagatc aacgacatgc
    16321 tgggtcacga gatcggcgac aagctgctga tcgaagccgc cgagcggatc cgcgccagcg
    16381 ttcgagatca agacaccgtg gctcggctgg gtggtgatga atttgcggtc atcctggaag
    16441 gcgtcagcga ggccaaagaa gtgcttccag tgatcgaacg aatcatcgcc agcctcagcg
    16501 aagttacgac gatcgacggc cacgaggtca acagctcgac gagcgtcggt atcgcgatgt
    16561 acccggaaaa cgggcgcgat ctgtccgagc tgcttcgagc agcagacctg gcgatgtacc
    16621 aggcgaagtc ctcgggtcgg ggccggtatc agttctttgc cgatgccatg caggaggaag
    16681 cccagtcgcg gcatgcgctc gaatgggcgc ttcgcagggc cgtggagaag gacgagttcg
    16741 agttggtcta ccaacctcag atctgcctac gcactggggt ggtcatcggc gtcgaagcac
    16801 tgatccgttg gcgaagtcca acgcgggggc tcctcacgcc gtaccacttc atctccggtc
    16861 tcgaagagtt cggtctgatc aacgaagtcg gcgagtgggt cctgaggacc gcctgcgagc
    16921 aggttcgaag atggcatgcg atggatttcg agccgatgcg cattggggtg aacgtgtccg
    16981 cccagcagtt cgaggacccg atgttgctcg acaagatccg gaacgcgctg aaggaaacga
    17041 aaattccgcc gtcgtttctc gagctcgagc tcaccgagag ctgcctgatg tcggatcccg
    17101 ccgaggccgg agcccttctt cgggagattc gagagatcgg cgtacgcatc gcgatcgacg
    17161 acttcggaac gggctactcc tcgctcacgt atctcaacga attcccgctg aacgcattga
    17221 agatcgacaa gaacttcgtc cagggcgtcg aatctaacga ccgtggcgga ccgatctcca
    17281 acatgatcat cggcctgggg cagaacttgg gtctcgaagt gatcgccgag ggagtcgaaa
    17341 ccgaggctca gctgatgtac atgcgcgagc acggctgtga catcgcccag gggtatttct
    17401 acgcataccc cgagtctccg gagaagctca ccgactggct caaggccaac cagcgtgcct
    17461 cggaaacgta cgttcgatcg attcccatca aaaagatcga caaggaaacg ggaacctgag
    17521 cgttccaatc cttgtgctag cgtagcagca tggcggccgc gacgattctc gtcgtcgacg
    17581 acgaacgaaa catcttgagc tcggtctcgc gtgcgctcgc gctcgagggc tacgaggtcg
    17641 aggtcgccgg aagcgccgaa atagccctcc agaaactcgc caagcagact ttcgaagcga
    17701 ttctgctcga cgttcagctt ccagggatgg acggcttgca gttgctcgac gcgttgcgcg
    17761 aaagggagat cagcaccccg gtcatcatga tgagcggaca tgcaacaatc gaagtggccg
    17821 tggaggcgac caggcgtggc gccgatgact tcatcgagaa gcccatcgga agcgagcgtc
    17881 ttctgttgtc gctcaagcga agcctcgagc tccgcgagct tcaacaggag aaccgcgagc
    17941 ttcgacggcg ctacggcgct ggtgaagcgc tgcttggatc gagcccttcg atgaaccgcc
    18001 ttcgcgagca gatcgagctc gccgctcgct caaacgctcc ggtcttgatt ctaggcgaga
    18061 gaggcaccgg gaaggagctc gtagcctcgg caattcacgc cgcctcagca cgagcgaagg
    18121 gaccgctcga gaagctcaat tgcgcggccg ttcccgaggg gctgatcgaa agcgagcttt
    18181 tcggacatga agcgggcgcc tttacggggg ccacgaagcg acgtcgcggc aagttcgagc
    18241 gcgcacacgg tggcactcta tttctcgacg aggtcgggga catgcctccg cagatgcagg
    18301 ccaagctctt gcgagtgctg caggaaggag aggtcgagcg cgtgggggcg agctcggttg
    18361 ccaaggtcga cgttcgggtc gtggcggcga ccaacaaggc gctcgaggcc gagatcgaag
    18421 cgggacgctt tcgtgccgac ctgttcgatc gtctgaacgt cgtgccgctt agagtgcccg
    18481 cactgagaga gcgcatcgaa gacgttccga tgctcacgga gcattttctc gccctggcct
    18541 gcgaggttca cgaccgaccc ggcaaggcgc tcgcgccagg ggccatcggc ttgctgaaag
    18601 cctaccccta tcctggaaac gttcgcgaat tgcgaaatct ggtggagcgc ctcgtcattc
    18661 tgacgccgga cgcgcgcatc agcgaagcgc aggcgcgcgc gcttctgcca atcgagtccg
    18721 agagcgcctc gggcacgcat tttcgacccg aaacgcctct tcgggagatg ctcgattcgg
    18781 tcgagcggga gctgatcacg aaagccctcg agttccgaga aggacacgtc accaagaccg
    18841 ccgccgacct cggtctcgag cgaagccacc tctacaagaa aatgcgcgcc ctcggagttc
    18901 ggaagcccga accggagtga gattgcgttc ttccccacgg caacatagag atccaactag
    18961 aagccgagga taacgtgttc gacaaactac tccccatcga tcccgaggtc gcagcccgca
    19021 tcgaagcgct cgatctccag ttcaacaagt tcggggtcga tccctacggc atagacaaga
    19081 ccgacctgac gcggttcgtt tcggcattcg cgtggatcta ccgcttctac ttcaaggtcg
    19141 acgtgtttgg aatcgagaac gtgccatcga cgggtcgcgg aatcctcgtt ggtaaccact
    19201 ctggcggggt cgcgatcgac ggggcaatgg tcatggggtc gatgttgctc gacgccgagc
    19261 cacctcgctt gccccatgca atgatcgaca agttcatcca cgagttccca ggggcgtccc
    19321 aactcatgag ccgaaccggg cagttcaccg gaaaccccga ccaggctgct cgcctgctgc
    19381 gcgcggagcg cttgattctg gcattcccgg aaggggcgcg gggcacggcg aagctcgcca
    19441 aggacgcgga ctcgctggtg ggtttcggaa ctggcttcat gcgcctggcg ctcgagacca
    19501 agagtccgat cgtgcctttc ggcttcgtcg gaggtggcga tgcgcttccg acgattgcga
    19561 atctgaaacg cctgggccgt ctcttcggcg tgccgtacgt gccggtcacg aagtggggtc
    19621 tcttgatacc aaagccgacg cgcttccaat tgctctacgg tagacccatg acgttcgagg
    19681 ggaccggtca cgagcgagac gaagtcgtcg agtccatggt cggtcaggtg aaagaacgaa
    19741 ttcgagactt gatccgacaa ggacgccagc tgagggagaa ggagatctcc gaagaagatc
    19801 tggtgctctg atgaaggttt tggtcacagg tatttctggg aagctcggac gaatcaccgc
    19861 cctgaagctt ctgaaacacg gctacgatgt gatcggtatc gatcgcaggc cctggcccga
    19921 agcgccggag gcgatcgagg tgtttcaggc ggacattcga aaacggcccg cggaagacgt
    19981 gttccgcacc catcggccgg acgcggcgat ccacatggcg acggtcaccc acttggtgca
    20041 caagagtccg gatcgcatgc gggtgaacct ctacggcacg cgcgcattca ttcaccattg
    20101 ccaccaatac ggcgtaaagc aggcggtttt tgcgggtcgc cacaccttct acggggcagc
    20161 cgcagactcg ccgctctatc acacggagga tgagcctccg atttcggccc ataccttccc
    20221 cgagctggcc gacctcgtcg cggcggatct ctacgccgcg tccgctctct ggcgctatcc
    20281 ggggatcgca accgcggtgc tccggatcgt gtatacgctc ggcccgagca agcatggcac
    20341 tttggcgtcg tttctgaagg gccccaacgt cccgttggtg atgggttttg atccgctctt
    20401 tcacttcatc cacgaagacg acgctgcgac ggcgatcgtg agcgcgctcg cccacaagct
    20461 tcacggcatc tacaacgttt cgggcccaac ccccgtgccg ctctccacga tcgtccgcgc
    20521 cgcgggccgc cgtgcaattc cggtccccga aaccatcttc aagctcggcg tcggccgcct
    20581 ggggctcccg cacctgccag ccggggcgat cgaccacctc aaatacccca tcgtggtcga
    20641 cagcgaagct ttccggcgag ccactcggta cacctacgaa ttcgacgaac gagaaaccat
    20701 caacagcttc cgcaccgcca agtagaccaa aggcgctggc aatgtggaga tcggaggacg
    20761 gctcgcgacg ggcgtgctcg cgattgggac ccactcgccc ccagctctct aagacgacct
    20821 acggctaggc tcgacccaat cgctgtgccg cccatcccga gccgtccgac ggaaccccgc
    20881 gtcgtgtttc gctgtgacct gtcgccggcg ctgaattgca gcttttttga cgggaccggg
    20941 tcctcacgtc gatctttctt ccgatgttgg tgtctgacga gcgaagcgag cttcgggggc
    21001 agctcctgga tggtcgttac cgcatcgggt ctgcgatcgg gatgggcgga accggcatcg
    21061 tgttcgaggc ggagcggctc gcgacgctgg agcgggtggt ggtcaagatg ctccggccca
    21121 ctcattccgg aaaccccgac ctgatgagcc gggtgcggcg cgaggtcgag gtggctcggc
    21181 gcgtattcca ccccggcatc gtgccggtca tcgaccatgg cgcgctctac gatggctcac
    21241 cttacctggt gatgaagcac atgcgctcgg agagcctggc ctcgtacctc cggcgccatg
    21301 ggcccctcaa cggacctcaa atctcggtga tcatggctcg gctcgcatcg attctgcaca
    21361 gcgtgcaccg gcatggctac gttcatcggg acatcaagcc cgagcacatt ctgctcaacc
    21421 gcggcggcta tggcgagctc gacgtgcacc ttctcgactt cggtgtctgc gccgccgaca
    21481 cggcccctcc agacgagcga gccagagagc gcggccgcgt ctacggcacc cccagctacg
    21541 cctcgcccga gcaggcgagc ggcaaccctt tcgtcgacgc acgcgcggat ctctttgccg
    21601 tggggatcac catgttcgag gcgctcacgg gaaagcttcc tttcacgggc gccgacgtca
    21661 cagcggtcct gagacgcatc attcgcgaag acgcgccgcg cgcgcgccat ctatcgccgt
    21721 acgtggacga cgagatggat ggcctcgttg ccaagcttct gagccgcagc cctgccgctc
    21781 gccatccttc ggcgcgagcg ttgatgcgag ccttggaacc ctggctcgtg gagcgcgttc
    21841 gaacagagcg cgcgcttgcg agcaccttgc aggtcgcccc ggataccagc agcatccggc
    21901 ccacggccca gcacgagatg gtcgcggcct agttgccagc taggcgatcg atcagagctc
    21961 cgacatccgc gggcgcagcc atggcatggt accccttgtc gacgtggaga accgtgccgg
    22021 tgatgccact cgcgagcgga ctacacaaga atgccgcggc gtgacccact tcctcggccc
    22081 gcagcgcgcc gggaagcggc gcgttcatct cgtagtactt gatcatcttg tggatgaagc
    22141 cgatcgcaga cgccgcacgc gaggcatggg ggccggcgga gatcaagttg acgcgatgac
    22201 catgcttgcg gccaagctcg aacgccaaca tcctggcgtc ggcttcgagc gcagccttgg
    22261 ccgatgccat tccgccgccg tagtctgaca ccacaccctg actcgcgagg tacgtgaggc
    22321 ccaagaacgc tccattctcg cgcatcaacg gcgctaggcg ttgcgctaat gacacgaacg
    22381 aaaaagcgct ggctccgtgc gcggcgagat agccacgtct gctcgtttgc atgagcggtt
    22441 tgttcacctc gggcccgttg gccaagctgt gaactacgat gtcgagcgcg gcgcccccaa
    22501 agtcctcgcg aagcctgttc gccaggccgt cgatgctgaa gtctccgcgg tcgcgatagc
    22561 gcttctggga gcggaggtcc tccggcgcgt cttcgagcga atcgaactcg gcgtcgagcg
    22621 ggtagatccg ttccagctcg agtgtgctcc cgtcgctcat ctgcaaggag gcatcgagct
    22681 tcccgcgacg cagcaaggtc tcgaagaccc cgagtgctgg cggccaggtg ccgacgcaaa
    22741 ccgtagcacc cgcttcggcg agagccttgg tgatggcaaa cccaaagccc gcatcgtcgg
    22801 ccacacccgc gacgaacgct cgtttccccg aaagatcgat aggcaacatg cgcggaacct
    22861 tatcgccagt cccggcaaac cgctacactc cgcgccatga agaaggagcg ccgaggagtc
    22921 gaatacggcg gagtcgacga aagctacttc gaggaccgcg gtttgcggcg ttacgccggc
    22981 gtatggtcgc tctgggcgct cggggtaggc gcggtgatct cgggggactt cttcggctgg
    23041 aacttcggcc ttgcctccgg gggctttggt ggcctcctca tcgccaccgt aatcatcacc
    23101 atcatgtacg ctggcctctg cttcagcatc gcggagatgg cagccgctct gccgcacacc
    23161 ggtggtgcgt actcgttcgc gcgctccgcg atgggtccct ggggggcgta catcaccggt
    23221 ctcgccgaaa acatggagta catcctcact ccggccgtca tcgtcgtggg catcggtggt
    23281 tatctgggcg ccatcttcgg cacccccgcc agcgtggagc cgctctggtg gcttgcttct
    23341 tacgccgtat tcgtcggact caacgtggcg ggcgtcgaga ccacgttccg attcaccgtc
    23401 ctgatcacag cgatggcgct aggcgttctg ctcgtcttct ggatcgccgc gattcctcat
    23461 ttcgacatga gctgggccct cgacatcgag cccgaacctg gccatgaccg ttggctgccc
    23521 aaggggatcg gtggtgttct ctccgcgctc ccattcgcct tctggttctt cctcgcgatc
    23581 gagcagcttc ctctcgccgc tgaagaagcg caggcgcctg cgcgcgactt accgcgaggc
    23641 cttttatacg gtctgctgac tctcatcgta gccgcgtttc tcaccctctt cctcaacgcg
    23701 ggtatcgccc cgggcgccga ggtcctcggc tcatcc
//
LOCUS       DASRZF010000055         2747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4855006, whole genome shotgun sequence.
ACCESSION   DASRZF010000055 DASRZF010000000
VERSION     DASRZF010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4855006"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /gene="pgsW"
                     /locus_tag="VFG22_02600"
     CDS             complement(<1..966)
                     /gene="pgsW"
                     /locus_tag="VFG22_02600"
                     /inference="COORDINATES: protein motif:HMM:TIGR04332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate system protein"
                     /protein_id="HET6415159.1"
                     /translation="MRGMYWKPEGASQVQRALVAALALVGLVAVEVFPTEEQQPYYAE
                     KMLAARKGQEALEVLRDASERRGLFLREKTDPSGSRMIGDVLSPITSGSGSLVSKQTT
                     VNPNFAAVVVQWLKDLDIKSGDLVAVGLSGSFPAMNVAVYSALYELGIEPIVISSTAA
                     SQWGANNPNFTWLDMEATLRKAEVFPYKSVAASLGGVGDDAIGLTERGRVMLERAIER
                     NKITALTEFEAKRPRSRAADDEEQSQAPELGLIDQDRVRERMRVYYEKAGDRPIKAYV
                     NVGGGTVSVGTKVGKRKFVPGVNSRPPKGIEDMPPSVLGAFLETGV"
     gene            complement(963..1421)
                     /gene="pgsC"
                     /locus_tag="VFG22_02605"
     CDS             complement(963..1421)
                     /gene="pgsC"
                     /locus_tag="VFG22_02605"
                     /inference="COORDINATES: protein motif:HMM:TIGR04011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein PgsC"
                     /protein_id="HET6415160.1"
                     /translation="MPEIELLTLSIGLGLAASLLYGELFGLAAGGMVVPGYVALYMNK
                     PVDIGLTLLAALMTFGFLKGVSQIAIVYGRRRTALTILIGYLVGLAFRMWLESPVTPF
                     SEGAIIGFIIPGLIAIWYQRQGVLETTLSLLTAAILVRMVLVVLIGDVTI"
     gene            complement(1408..2604)
                     /gene="pgsB"
                     /locus_tag="VFG22_02610"
     CDS             complement(1408..2604)
                     /gene="pgsB"
                     /locus_tag="VFG22_02610"
                     /inference="COORDINATES: protein motif:HMM:TIGR04012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate synthase PgsB"
                     /protein_id="HET6415161.1"
                     /translation="MSDEIVILLTACGVLILLGALELILHRRRLARIPIRIHVNGTRG
                     KTSVTRLIAAGLREAGIRCVAKTTGTVPRFILPNGREVPVYRPGGANIIEQKQSVTMA
                     AAQRAEALVVECMALQPQLQWLSESKLVRATHGVITNARPDHLDVMGPTEDDVARALA
                     ATVPVGGVFLTAERKHREFFQQVAEDRGSRFHASEDIVRPLRPEDLEGFSYLEHQENV
                     ELALAVCEEIGIDTETALRGMWRAKPDPGAMTEARIEFFGRHIVFVNGFAANDPFSTR
                     YIWEQSLRRHPELERRVAVFNCRSDRADRSEQLGEAYGSWTQADEVVLLGTGQYIFAR
                     AAVQSGLDPTRLVFVDQTHPADIFEILLGLAETSALIMGLGNIAGPGLALAEYFANRG
                     IENARD"
BASE COUNT          543 a          867 c          834 g          503 t
ORIGIN      
        1 caccccggtc tccagaaacg ctccgagcac cgaagggggc atgtcctcga tgcccttggg
       61 cggcctggaa ttgacgcccg gaacgaactt gcgcttgccg actttcgttc cgacggagac
      121 cgttccgccg ccgacgttga cataggcctt gatcggacgg tcacccgcct tctcgtaata
      181 cacgcgcatt cgctcgcgga cgcgatcctg gtcgatgagt ccgagctcgg gcgcctgcga
      241 ttgctcttcg tcgtcggctg cacggctcct aggcctcttc gcttcgaatt cggtcagcgc
      301 ggtgatcttg tttcgctcga tggcgcgctc gagcatgact cgacctcgtt cggtgagccc
      361 gatcgcgtca tctcctacgc caccgagtga cgccgccacc gacttgtacg ggaagacttc
      421 ggccttgcga agtgtcgctt ccatgtcgag ccaagtgaag ttgggattgt tcgcgcccca
      481 ctgggaggcc gccgtgctcg aaatgacgat cggctcgatc ccgagctcat atagtgccga
      541 atagaccgcc acgttcattg cgggaaaaga tccggataat ccgaccgcga cgaggtcgcc
      601 gctcttgata tccaagtcct tgagccattg cacgaccact gcggcgaagt tgggattcac
      661 cgtagtttgc ttcgaaacca agctaccgct gccgctcgtg atcggtgaca ggacgtcgcc
      721 gatcatcctc gacccggatg ggtcggtctt ctcgcgcagg aagagacctc gtcgctcgga
      781 ggcatcacga aggacctcca aagcctcttg tcctttgcgc gccgcgagca tcttttcggc
      841 gtagtacggc tgctgctctt ccgtgggaaa tacctcgacc gcgaccaggc ccacgagggc
      901 cagcgccgcg accaaggcgc gctgtacctg agatgcgccc tcgggcttcc aatacatccc
      961 tctcatatcg tcacgtctcc gatcaaaacg accaggacca tgcgcacgag gatcgcggcg
     1021 gtcaggaggc tcagcgtggt ttcgagcacg ccctggcgct gataccaaat cgcgatcaac
     1081 ccgggaatga tgaagccgat gatcgctccc tcggaaaacg gtgtcacggg gctctctaac
     1141 cacatccgaa atgccagccc aacgaggtac ccgatcagaa tggtgagcgc cgttcgccgg
     1201 cggccgtaga cgattgcgat ctgggaaacg cccttcaaga aaccaaacgt catcagagcg
     1261 gcgagcagcg tgagtccgat gtcgacgggc ttgttcatgt aaagagcgac gtagcccggc
     1321 acgaccatgc cgccggcggc gaggccgaag agctctccgt agagaaggct ggcagcgagg
     1381 ccgagcccga tcgacagggt caacaactca atctctggca ttttctatcc cccgattggc
     1441 gaagtattcg gccaacgcca agccaggccc ggcgatgttc ccaagcccca tgatcagggc
     1501 cgaggtttcg gcaagaccca acagaatctc aaagatgtcg gccggatggg tctggtcgac
     1561 gaaaaccaat cgcgtaggat cgagaccgct ctgcaccgcg gcgcgggcga agatgtactg
     1621 gccggtgccg aggaggacga cttcgtcggc ctgcgtccag ctcccgtacg cttcgcctag
     1681 ttgctcggag cggtcggcgc ggtccgacct gcaattgaac accgccacgc gacgttcgag
     1741 ctcggggtgg cgccgcaagg actgctccca gatgtaacga gtcgaaaagg ggtcgttggc
     1801 cgcgaagccg ttgacgaaca cgatatgtcg accgaaaaac tcgatgcgag cctcggtcat
     1861 ggcaccggga tcgggcttgg ccctccacat gcctcgaagt gcggtttcgg tgtctatgcc
     1921 aatctcttcg cagaccgcga gtgcgagctc gacgttttcc tgatgttcca ggtaagagaa
     1981 gccctcgagg tcttcgggac gaagcgggcg aacgatgtct tcggaggcat ggaagcgaga
     2041 acctcggtcc tcggcgactt gctggaagaa ctcccgatgc ttacgctcgg cggtcagaaa
     2101 cacaccgccc actggaacgg tggctgcaag cgcgcgcgca acgtcgtctt ccgttggacc
     2161 catgacgtcg aggtgatcgg gtcgcgcgtt ggtgatcaca ccgtgggtag cgcggacgag
     2221 cttcgattcg gaaagccatt gaagctgcgg ctgaagcgcc atgcactcga ccaccagcgc
     2281 ttcggcgcgc tgcgcggccg ccatggtgac ggattgcttc tgctcgatga tgttcgcccc
     2341 accaggccga tagaccggaa cttcacggcc attcggcaag atgaagcgag gaaccgtgcc
     2401 ggtcgtcttc gctacgcatc ggatgcccgc ctcgcgaagg ccggcggcga tcaagcgcgt
     2461 cacgctggtc ttaccgcggg tgccgttcac gtggatgcga atcggaatcc gcgcgaggcg
     2521 ccgtcggtgc aagatcagct cgagcgcccc cagcagtatg aggacaccgc acgccgttag
     2581 aaggatgaca atctcgtcag acactcgact cgccccaatc ggctcgaaag cccccgaacg
     2641 cgagcccttg taccagcttg caccgtctca gcaagcctgc cgcggcctga agtcggacga
     2701 gcgctcgatc gaggctagcc cagcagtgcc gctgaaattg taatgaa
//
LOCUS       DASRZF010000056         7115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3480078, whole genome shotgun sequence.
ACCESSION   DASRZF010000056 DASRZF010000000
VERSION     DASRZF010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 7115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7115
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3480078"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1761)
                     /locus_tag="VFG22_02615"
     CDS             complement(<1..1761)
                     /locus_tag="VFG22_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415162.1"
                     /translation="MKDDNGVWWFQRPDGSRFYSLGVNNIEPEPFMPRPGTDYYNPVP
                     TEFNGDTHAWASFVRNLLTELGFNTVGAWSSAVLPTDERFVATPILYVVLHDDTRCLA
                     PLQPDFESFVQQNVKESIAKLPGREGLLGVFLDNEMPWFGKTGFQYIPTYTLLELAFE
                     LPTDDARHKAALEFLESRHGSVESLAAAYGQPVHSWDDVTVKLLHGSTSPEAAADREQ
                     FTAMLAERFFEVSSRIVRQELPGVLVLGSRFAGDAPDSVIRAAGKYCDVISVSDFRFV
                     PKADENTLARFWLLGGRPLMHVDFSFRARANASGNLNTYGVGPVFDTQADRAAAYAAL
                     VSDTATVPYVIGSHWFGFADQSPQGRFDGEDGNYGIVDIRNQPYPEMVAAMKATNARV
                     PALHASTKRVMPTELRPRMAASYSPGQHPERPPTLGLLGDWVAPPETWAAPDAKMSWE
                     MRAGELILNYETGTQYGAGINLFGPKANAIGHGPSLATDLDGYASILIDVTAPKGLQL
                     NIVLAEAGSGMMWTPRFDTSAGDDGEAFISIPIFGDGVRTTHRLPIGGLLLQQFYGNQ
                     AGARRIDMQALRNIGVVVTGE"
     gene            1784..1930
                     /locus_tag="VFG22_02620"
     CDS             1784..1930
                     /locus_tag="VFG22_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415163.1"
                     /translation="MAGAAGGRPPVTAQLAELVRMTARRAVVAASRMGERMEQESLGR
                     RRRV"
     gene            complement(1927..2232)
                     /locus_tag="VFG22_02625"
     CDS             complement(1927..2232)
                     /locus_tag="VFG22_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415164.1"
                     /translation="MAQALTAIIGHGKIELLRRGSTEESRSVSQALPKRSSRANSHPG
                     PSLAEATDGPRDSDTDLDCSEGESARFRAAHTDEIPDTPRQRLEVLGEQAVVCLEGR"
     gene            complement(2561..2713)
                     /locus_tag="VFG22_02630"
     CDS             complement(2561..2713)
                     /locus_tag="VFG22_02630"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET6415165.1"
                     /translation="MFGSNFPMDKVSIDYALLISGLDALPSTRADEEKAKFFAENARE
                     FYAIGG"
     gene            complement(2730..4022)
                     /locus_tag="VFG22_02635"
     CDS             complement(2730..4022)
                     /locus_tag="VFG22_02635"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET6415166.1"
                     /translation="MRSLPFIGPVRQFLGDVLPFLMESRATYGDAFRLRMFNLEMTCL
                     CGLEAIELLEAGSCLRTSKSMHVLDGELGSCLPSTFDGPQHRMFRKAHLEFMTASLEA
                     KRRADIQKRLVEHTSEWRAGDRVDVLHEAQVQTVDLLSVVLNGEPFPFSKKDLALVVH
                     TLIWATFGHAPSWVLRNPVYRSVQKRMRSHFLDLVARIRTSPERAATLVGRYLDLEPP
                     IGRERWEDRDLVAVPYSAYLAGFDTVASASSFLLYRLLTHPEHLARVREEFEELSLEL
                     PGPVAPTKQKFLRAAFLEAVRLNPPGTAVIRVADRDFEFAGYTIRKGDEVLVVIASDH
                     RNPAVFERPNEFDPTRYFAPQSGPLRRRVLPFGSGAHRCTGAVLGELVAVEMVSYWVN
                     NFDLQVVPPGRKVRAVARPFTQPEGLRVKVLGRRDALA"
     gene            complement(4241..4843)
                     /locus_tag="VFG22_02640"
     CDS             complement(4241..4843)
                     /locus_tag="VFG22_02640"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6415167.1"
                     /translation="MNGDPTRQKLLHEAARHFARHGYDGASLRRISEGVGIRAASIYN
                     HFPDGKEELYEHIMSAIAEMLMDRIVNRYGRNVGLSAEDAIVQLGAAFWDFCEEHPDY
                     ATLLIREMFDNREPDVGALMGRAPEIIEASLGYIENAQASGELPEFDAEAFILWSASY
                     LLSYHGAPGLRTHVQRKAWSKELARDHFITMLRKLLQTAD"
     gene            5046..6230
                     /locus_tag="VFG22_02645"
     CDS             5046..6230
                     /locus_tag="VFG22_02645"
                     /inference="COORDINATES: protein motif:HMM:NF039286.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET6415168.1"
                     /translation="MRIKDVENKAFFAQFIARLFVHNVLKDSQIFLPKGDYERRHGDF
                     PRYFSGWYLFVPSDDLAEGEIVHKKLNGLDLVAYRNKEGQPVVHSNRCTHMDGMFAPL
                     GSVQDGRIQCAYHGYTFENGKPRSGPAEFRNDPSKCIPCHPVTEVNGLIFFWFDAKSE
                     DGVGVPTWELDLPDVSRFPRRATMRSITPTHMAPLHENIIDDQHFMILHGSQRYKSDL
                     LPYQHKHRFRTRNRMVFELPDRMKKFFRLEENPVTEMDSDFHGLGIHITKANIGGFEA
                     LMIHCTTPIEDEVTEWTLTMYMDKRQWSLIPSYRDFISQIHPWGTFAQVYYLHTQDRR
                     AFFEKAPYRFYEDVPKGFEKVNAFRRWIQVELLGEERPMGKNCIPTKYTPLNVLQDDA
                     AE"
     gene            6310..6615
                     /locus_tag="VFG22_02650"
     CDS             6310..6615
                     /locus_tag="VFG22_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6415169.1"
                     /translation="MVVVTGGKDGLGFQSAKALARNEAEVILACRSTERGQNARREIL
                     GVVPHANVRVMKLDLQDLSSIEAFANEFNESSDRLDVLLNNAAIMLVSPLNSVGTGL"
BASE COUNT         1324 a         2134 c         2354 g         1303 t
ORIGIN      
        1 ctcgcccgtg acgaccaccc cgatgttgcg cagggcctgc atgtcgatcc tgcgggcgcc
       61 ggcctggttt ccatagaact gttgaaggag cagcccgccg atggggaggc ggtgggtggt
      121 ccggacgcca tcgccgaaga tggggatcga gatgaaggct tcgccatcat cgccggccga
      181 ggtgtcgaac ctgggcgtcc acatcatgcc ggaaccggcc tcggccagca cgatgttgag
      241 ttgcagaccc ttgggcgcgg tcacgtcgat caggatgctc gcgtagccgt cgaggtcggt
      301 cgccaacgac ggaccgtggc cgatggcgtt ggccttgggg ccgaagaggt tgatcccggc
      361 gccgtactgc gtccccgttt cgtaattcaa gatgagctcg cccgccctca tctcccagga
      421 catctttgcg tcgggcgcgg cccaggtctc cggtggcgcg acccagtctc cgaggagtcc
      481 gagcgtgggt ggacgctcgg ggtgctggcc gggcgagtac gaagccgcca tgcgcgggcg
      541 cagctcggtg ggcatgaccc gcttggtgga ggcgtgcagc gcaggcacgc gggcgttggt
      601 ggccttcatg gctgcgacca tctctgggta cggctggttg cggatgtcca cgatgccgta
      661 gttgccgtcc tcgccgtcga accggccctg cggcgactgg tcggcgaacc cgaaccagtg
      721 cgacccgatg acgtagggga cggtcgcggt gtcggagacg agcgcggcgt aggccgcggc
      781 ccggtcggcc tgggtgtcga acacgggtcc cacgccgtag gtgttgaggt tgccgctggc
      841 gttggctctg gcccggaatg agaaatcgac gtgcatgagc gggcggccgc ccagcagcca
      901 gaagcgggcg agcgtgttct cgtcggcctt gggcacgaag cggaaatcac tgaccgagat
      961 gacgtcgcaa tacttgccgg cggcgcggat gaccgagtcg ggggcatcac cggcgaagcg
     1021 ggagcccaag accaggaccc cggggagctc ctggcggacg atgcgggagg aaacctcgaa
     1081 gaagcgttcg gcgagcatgg cggtgaactg ctcccgatcg gccgcggctt ccggggaggt
     1141 agaaccgtga agcagcttga ccgtcacgtc atcccaggag tggaccggct ggccataggc
     1201 cgccgccagg ctctcgaccg agccgtggcg cgactcgagg aactccaggg cggccttgtg
     1261 gcgcgcgtcg tcggtgggga gctcgaacgc cagctccagg agggtgtagg tcgggatgta
     1321 ctgaaacccg gtcttgccga accagggcat ttcgttgtcc aggaacacgc cgagcagtcc
     1381 ttctcgaccg gggagcttgg cgatcgactc cttcacgttc tgctgcacga acgattcgaa
     1441 gtccggctgc agaggggcga ggcagcgggt gtcatcgtga agcaccacgt acaggatggg
     1501 cgtggcgacg aagcgctcgt cggtcggaag gacggcggag ctccacgccc cgaccgtatt
     1561 gaatccaagc tcggtgagga gattgcgcac gaagctggcc cacgcgtggg tgtcgccgtt
     1621 gaactccgtg ggcacggggt tgtagtagtc cgtgcccggt cgcggcatga acggctcggg
     1681 ctcgatgttg ttcactccga gggagtagaa gcggctgccg tcgggccgct ggaaccacca
     1741 cacgccgttg tcgtccttca cgaccgagta gaggggtgcg ctcgtggccg gtgccgcggg
     1801 cggcaggccg cccgtcacgg cgcagcttgc tgagctcgtg aggatgacgg cgagaagagc
     1861 ggtcgtggcc gcaagccgca tgggggagcg catggagcag gagtccttgg gtcgaaggcg
     1921 ccgggtttag cggccttcga ggcagactac cgcctgttcg ccgagcactt caaggcgctg
     1981 acggggagtg tccggtattt cgtccgtgtg cgctgctcgg aatcgggccg actcgccttc
     2041 cgagcagtcc aggtcggtgt cggagtcccg agggccgtcc gtggcctcag cgagcgacgg
     2101 gcctggatgg ctgttggcgc gactggatcg cttgggaagc gcctgggaca ccgacctgct
     2161 ttcttcggtt gagccgcggc gcagaagctc gatcttgccg tgacctatga ttgcggtcag
     2221 tgcctgagcc atgaagcctt tcccatcctg ccgcgcgttt ctcttcggga gcctcgtcgc
     2281 ttgttcgctt ctcctggcag gctgcccgac aagcggcacg caatcgccgg cccccgccgc
     2341 ggcttgcgcg gaacgctacg ccaagtgccg aatgcccgat gggccgctcg gggtgtgcaa
     2401 tgacgcgccg tgcccagagg gcgagacccc gccctgcttt cagtgcgttt cacagcatta
     2461 gtcccagtga gcatcgcaga acgcgagatg cggcggggcg cgacgtttgg gccgcatgct
     2521 gcgtcgaggc agccgggcag cacgggcagc attcgcgtca tcatccgccg atcgcgtaaa
     2581 actcacgggc gttttcggcg aagaacttgg ccttctcttc gtcggctcgt gtcgacggga
     2641 gcgcgtcgag gcccgagatg agcagggcat agtcgatcga gactttgtcc atcgggaagt
     2701 tcgatccgaa catgcaaccg tcgatgcttt caagccaacg cgtctcgtcg accgagcacc
     2761 ttcacgcgca ggccttcggg ctgcgtgaag gggcgtgcca ccgcgcggac cttgcggccg
     2821 gggggtacga cctggaggtc aaagttgttc acccaatagg aaaccatttc gacggccacg
     2881 agctcaccga gcacggcgcc ggtgcagcgg tgggcgccgc tgccgaaggg gagcactcgt
     2941 cgtctcagcg ggccagactg cggtgcgaaa tagcgcgtcg ggtcgaactc gttcggacgc
     3001 tcgaagactg ccgggttgcg gtgatcgctc gcgatgacga ccaggacttc gtcacccttg
     3061 cgaatcgtgt agccggcgaa ttcgaagtct cgatccgcga cgcgaatgac cgccgtgccc
     3121 ggtgggttca gccgcacggc ttcgaggaag gcggcgcgga ggaatttctg cttggtggga
     3181 gcgacagggc cggggagctc gagagagagc tcctcgaatt cctccctgac ccgagccagg
     3241 tgctccgggt gggtgaggag ccgatagagt agaaaagagc tggccgacgc gacggtatcg
     3301 aagcctgcca gataggcgct ataaggcact gcgacgagat cgcgatcctc ccaacgctcc
     3361 cgcccgatgg gaggttcgag atcgaggtaa cgccccacca gcgtcgcggc gcgctcgggg
     3421 ctcgtgcgaa tccgcgcgac tagatccaag aagtgcgaac gcatgcgctt ctgcacagac
     3481 ctgtacaccg gattccgcaa gacccacgaa ggcgcgtgcc cgaatgtggc ccagatcaaa
     3541 gtgtgcacga ccagggccaa gtccttcttg ctgaatggaa agggctcgcc atttagaacg
     3601 accgacagga ggtcgacggt ttgcacttgg gcttcgtgaa gcacgtccac ccgatcgccg
     3661 gcgcgccatt ccgaggtgtg ctcgacgagg cgtttctgaa tgtcggcccg gcgcttcgcc
     3721 tccaagctcg cggtcatgaa ctcgagatgt gcctttcgga acatccggtg ctgggggccg
     3781 tcgaatgtac tggggagaca actcccgagc tccccgtcga gcacatgcat cgacttgctc
     3841 gtccgcaaac agctcccggc ttcgagcaac tcgatggcct cgaggccaca cagacaggtc
     3901 atctcgaggt tgaacatgcg gaggcgaaac gcatccccat aggtggcgcg actctccatc
     3961 aggaacggga gaacgtctcc aaggaactgc cggaccgggc cgatgaatgg caatgacctc
     4021 accagcggcg gacgacgttg ctcgacgcga cggcgtttct tgcttgacgt tggctcgttt
     4081 gcggtagtcg cggtcaaaaa aagggtcctt tttggctgct aacagttgtt agtttgaggc
     4141 taatatctgt ttcaccgatg cgcaatggta agttttcgag cgccaatcgc gagctcggcg
     4201 ccgccatgtc tggtgtgaac cggtccgttt cagcgtacgg ctagtctgct gtctgcaaga
     4261 gcttccgcag catcgtgatg aagtgatccc gagcgagctc tttgctccac gccttgcgct
     4321 gcacatgggt ccgcaacccg ggggccccgt ggtagctcaa caggtagctc gcgctccaca
     4381 ggatgaacgc ttccgcgtcg aactctggca gctcgccgct ggcctgcgcg ttttcgatgt
     4441 agccgaggct tgcctcgatg atttcaggcg cgcgacccat gagcgcaccg acgtctggct
     4501 cgcgattgtc gaacatctct cgaatcagta gtgtcgcgta gtcggggtgc tcctcacaga
     4561 aatcccagaa cgcggctcct agctggacga tcgcgtcctc tgcggacagg ccgacgttgc
     4621 gcccgtaccg attgacgatc cggtccatca gcatctcggc gatcgccgac atgatgtgct
     4681 cgtacagctc ttctttcccg tccgggaaat ggttgtagat cgatgccgcc cgaataccga
     4741 caccctcgga aatccgccgc aaagacgctc cgtcatagcc gtgtcttgca aagtggcgcg
     4801 cggcctcgtg cagaagcttt tggcgggttg gatctccgtt cacacctgca ataaacgcga
     4861 aattcgttgg gttcacaacg gaacagttgg gttggtgtcc cccggtcccc gcgattccgc
     4921 gggcacggag ccgagccgcc aggaaagggc ttgcgcgaaa gctccgaccc ctctagacta
     4981 acgatcgtta gtctcaggca tttatgcgtt agtcttgggg ccggccgcct gttgaggagg
     5041 agttcatgag aatcaaagac gtcgagaaca aagcattttt cgcgcaattc atcgcgcggc
     5101 tcttcgtgca caatgtcctc aaggactcgc agatcttcct accgaagggc gactacgaac
     5161 ggaggcacgg cgacttcccg cgctacttct ccggctggta tctgttcgtg ccctctgatg
     5221 acctcgctga gggcgagatc gtgcacaaga agctgaacgg gctcgacctg gtcgcctatc
     5281 gcaacaagga ggggcagccc gtcgttcaca gcaaccgctg cacgcacatg gacggcatgt
     5341 tcgcaccgtt gggctcggtc caagacggtc gtattcagtg cgcgtaccac ggctatacgt
     5401 tcgagaacgg caaacctcga tcggggcctg cagagttccg caacgacccg agcaagtgca
     5461 ttccttgtca tcccgtcacc gaggtcaacg ggctcatctt cttttggttc gatgccaaga
     5521 gcgaagacgg cgtcggcgta cccacgtggg aactagacct gccagacgtg agtcgtttcc
     5581 cacgacgggc gacgatgcga tcgatcacgc caacacacat ggcgccgctg catgagaaca
     5641 tcatcgacga tcagcacttc atgatcctcc acggatcgca gcgctacaag tcggacctct
     5701 tgccgtacca acacaagcac cgatttcgca cccgcaaccg aatggtcttc gagcttccgg
     5761 atcgcatgaa gaagtttttt cgcctcgagg aaaacccggt cacggaaatg gactcggatt
     5821 ttcatgggct cggcatccac atcaccaagg ccaacatcgg cgggttcgag gccttgatga
     5881 tccactgcac cacgccgatc gaagacgagg tcacagagtg gacgctcacc atgtacatgg
     5941 acaagcgaca gtggagcctc atcccgagct accgcgactt catcagtcaa atccatcctt
     6001 ggggaacgtt cgcacaggtc tattacctgc atacgcaaga tcgccgcgcg ttcttcgaaa
     6061 aggcgcccta ccgtttctac gaggacgtgc ccaaaggctt tgaaaaggtc aacgccttcc
     6121 gtcgttggat tcaggtggag ctgctgggcg aagagcgacc gatgggcaag aactgcattc
     6181 ccaccaagta cacaccgctg aacgtgttgc aagacgacgc cgcagaataa ccgtcctctc
     6241 gccgcacata cgaagcgctg gagagtggcc gagtagacac cgaacgacat ccccaatctg
     6301 cggggtagga tagttgtcgt caccggtggc aaggacgggc tgggcttcca atccgcgaag
     6361 gcgcttgccc gcaacgaggc ggaggtgatc ctcgcctgcc gctcgacaga acgcggccaa
     6421 aacgcgagac gagagatctt gggggtcgtg ccgcacgcca acgtgcgcgt aatgaagctc
     6481 gacctgcagg acttgtcgtc gatagaagcg ttcgctaacg agttcaacga gagctcggac
     6541 cgcctcgatg tcttgctcaa caacgccgcg atcatgttgg tgtcgccttt gaactccgtg
     6601 ggcacggggt tgtagtagtc cgagcccggt cgcggcatga acggctcggg ctcgatgttg
     6661 ttgactccga gggagtagaa gcggctgccg tcgggcccct ggaaccacca cacaccgttg
     6721 tcgtccttca cgaccgagta gaggggtgca gtcgtggccg gtgcggccgg cggcaggccg
     6781 cctgtcaggg cgcagcttgg tgagctcgtg aggatgaagg taggaagagc ggccgtggct
     6841 gcaagcggca ggggggcgcg catggagccg gagtccttgg gtcgaaggcg ccgggtttag
     6901 cggccttcga ggcatactac ggcctgttcg aggactcgtg tgtgactgga atagcaggcc
     6961 cctgcaaggg gccgagtaca ccgtcggttc gatgtccccg tagatacgtg tgaaacgttt
     7021 acagagagct cggtgcggaa gagaggactc gcgtgcgctc gggatatggc agggcggaat
     7081 ggacgcagtc catggagctg ccagattcct gtgcg
//
LOCUS       DASRZF010000057         9499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2447939, whole genome shotgun sequence.
ACCESSION   DASRZF010000057 DASRZF010000000
VERSION     DASRZF010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 9499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9499
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2447939"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VFG22_02655"
     CDS             complement(<1..327)
                     /locus_tag="VFG22_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415170.1"
                     /translation="MSGGEVEGDRRYRDTWLRPVRVAPLRLHLKRADVAQGAVRLRSG
                     LALVGDWWLTFRIDAPCLGVADDLVPPSFLRLLSPAAYPARFPRTDVVEIGVIVVHII
                     WVKVGRV"
     gene            complement(849..1223)
                     /locus_tag="VFG22_02660"
     CDS             complement(849..1223)
                     /locus_tag="VFG22_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415171.1"
                     /translation="MLLRLAIVSLLVISGVACKHNKRANTTVRERAAFDLLCDPNELK
                     LGVIDTEGARKLVSQIAVYGCDKKAVYVYYPDADTWVIDGAITEVPEDFKMEERSGES
                     RNKKSKRKAQKAEKKGKMDSGY"
     gene            1418..2224
                     /locus_tag="VFG22_02665"
     CDS             1418..2224
                     /locus_tag="VFG22_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415172.1"
                     /translation="MLCAVTTMSCAETLDIPVAPDLDDVVVAYASPSAKISGTVMEAV
                     GDELLELRELLKQSEVFEEILDVIVSVQLELDANTDESGNLEVPGLGAFPEPNAVIEI
                     NHDCAGWERNAAEGDAETAGSIRLTMLLDTGDVRPEVWGDATQCKFLAALGATTLQSS
                     YDGQVAVHFGEEPVPTGERLRDLVITFVLTGTLGVAGKEVPIDQSFRLRGEGDLEILW
                     ELNDGTSFVYLFNLETPRQGIRDANCTGDEGCSCSLEERECTLPSGSIFW"
     gene            2191..3096
                     /locus_tag="VFG22_02670"
     CDS             2191..3096
                     /locus_tag="VFG22_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415173.1"
                     /translation="MHPSLRKHLLVAVICTMSAGASAVPSKARAYHTYKERLLDTTAY
                     SLERREFRLGAMKLSYGIFKYLEISTYTVPWLAGLFLEEFETIGSPNVEFKSTLINRN
                     RFALSVSVEFLWGRVSSCTNPMLNPDGQLCTTEAEFSTVNWLVYPISVTSSVRINSRI
                     SIHIGGQYTATQATGGTRPFEGDISGSAVVNMLQIYGMFEWRLSRVVALTVTTRWLPY
                     VSQTVVRGQIVVDDDTGATIGIQADILDNNAFAVIPGAVFSWNRANIRLGVGYGAFFV
                     EPVGLVVPASVLSSISPEFDVFVRF"
     gene            3457..4107
                     /locus_tag="VFG22_02675"
     CDS             3457..4107
                     /locus_tag="VFG22_02675"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HET6415174.1"
                     /translation="MEPLPRFVPLDRMVAFSDGVFAVAITLLVLGIEIPSDAVLTGSA
                     LVMEREKLLHQLFIYFVAFWLVGMYWSQQSLFTSGLKQIDRGTFVLNLLLLLPITLLP
                     FVTQFMGTMRADWRAVLLFALTNVLATLVLVSMWSRIAALPEIHKSPQTATLAARMRV
                     EVRVHAAVMVVGVLVARLDVRVGTLVFILIPFAHFYNFLRDPFRSRLGGLSSGEPK"
     gene            complement(4176..5687)
                     /locus_tag="VFG22_02680"
     CDS             complement(4176..5687)
                     /locus_tag="VFG22_02680"
                     /inference="COORDINATES: protein motif:HMM:NF019045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Na+/H+ antiporter"
                     /protein_id="HET6415175.1"
                     /translation="MSRLIIGLGKVVGVTALLMLAACPALAAASSGHTGPGIDFPPAL
                     EDYGDADLTSVMAILRHRIEVVPFNLWATLIFIGAIVHTFLTYRFRNIARVLERRHEE
                     KVRTGATQTRVSASARVFDFLGDAEAVFGIWGIPLFYTIVVFYDRATAVAYINEGVSF
                     TEPVFVVVIMTFASTRPILELTERALRLLARLGGETPAAWWLVLVAVGPLLGSFITEP
                     AAMTICASILSSKFYAHKPSKPLAYGTLGLLFVAISVGGTWTNFAAPPVLMVADAWGW
                     TTGHMMSHFGWKAALGTGLTTAAYYLFFRHELNRMRDDPAATKHEGGDDLPIPFWVTA
                     GHVLFMLSAIANAHNPKQLIFVLLFFLAFVKITEDFQRNFNLRPAVLVGFFLAGLVIH
                     GGLQQWWIAPVLGSLDEIQLALGTTVLTAFNDNAAVTYLCTLVPTMTDGMKYAAVAGA
                     VTGGGLTVIANAPNPAGQSILSKHFEGGISAVGLLAGALLPTLMVLGCFLVFR"
     gene            complement(5790..6752)
                     /locus_tag="VFG22_02685"
     CDS             complement(5790..6752)
                     /locus_tag="VFG22_02685"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HET6415176.1"
                     /translation="MKVSCYRLDAGPTLAATSKVEALDRWRAGDGVFWLDVSEYDRES
                     LEGLLEEVGATGFLRQRCLEQARGTSAFALPEGTFAEWTMFLDRQCTKRGHASALCLE
                     NLLVTMHAEPIEGTQDMNRAVGFLELERVSTAGVLCAILLRHGARTARTTRAIRDRLL
                     ELGQLMDEDIGAVDPLELERLKRSVMLTDEVAEEQEEAFELIEQAQSKGLDLSSLQAP
                     LRLLRATASASFRLNERMDGRVENLLRRIQDQKQEHLNQRLGLLTIVSAIFLPLTLLA
                     GIWGMNFEEMPLLSDPVGYRIALATMATLGVGSAWFFYKRGWFS"
     gene            complement(7208..9361)
                     /locus_tag="VFG22_02690"
     CDS             complement(7208..9361)
                     /locus_tag="VFG22_02690"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET6415177.1"
                     /translation="MDTKTRWVGLGVLGFLVLFQVSPCDEDLGDCGPAIHSDGVQSCE
                     DWSEDYVLDPGDSDVTVSQVEVKNFLEELSSFGASMPAARATASEEPTPATQAESIER
                     VQAYVLEYAENRATDEPIEVVLDMGDLPFPELPQLQAMDAASRDRVLADRKALIEREQ
                     QGIKAYLDQLGARTTKPVGLLNHLQAWIAPDAIADVAAHPDVKAIHPAWQPIILLYDQ
                     EEVRKATFLSEYWDQEIKGESKSRICEPAAGYEDIKIAVVEASISSFVLNRLNRVHPG
                     WGDCVFGSCESRVRAELNCSFVSEDLDACLPWTGDLQTLANHGTWVTSIAAGDLTQGQ
                     DPRTTDPDEQRRRTGIAPEASILYLNASSTGFVAAAVATAFLLGADVINLSLAVTECA
                     FDLTDLTADCGGLNQVIRTVTKGGALVVAAAGNENSEQGRCEPTSGCSVCYPAVRPEV
                     LAVGNVETSQGIDYDLAEIAENSSRGFARVGVGGQHSADYPSGVDIPVVSIAAPGNLC
                     AYFNNDDTYDEVGRSGTSFSAPVISAAAGLVREEFGPAVWDARMLKSHVLTMGDGSGA
                     DVSQPGVAVGTSSVWGTGRVKMHPFDAMQSPKGLAHRSFRIHENERVLFHAADTKGDA
                     LEPDVTQWKMGMYIDWPDLSTIPYLLISYWNTCNRPRLIAADLNPGLERHVVLESPAI
                     DQACLEIRVYGYSVPAGGVEVFVTDYYHSGDPSEH"
BASE COUNT         1910 a         2874 c         2793 g         1922 t
ORIGIN      
        1 gactcgacca accttgaccc agatgatgtg gactacgatg acgcctatct ccacgacgtc
       61 cgttcggggg aaacgagctg ggtaagccgc cggtgacaaa aggcgaagaa acgacggagg
      121 gaccaggtcg tccgcaactc cgaggcaggg tgcgtcgatg cggaaagtga gccaccaatc
      181 gccgaccaac gcgagccccg accggagacg gacggcaccc tgtgcgacgt cggcgcgttt
      241 gaggtgcagc cgtaggggcg ccacacgcac tgggcgaagc caggtatctc tgtaccggcg
      301 atcgccttcc acttccccac cggacactcg atgggaatgg ttccgcaaat tcgatcgatc
      361 ttgccccggt caaaccggac tcgctcgtga aagacggaaa gacgcggaca ttcacgaccg
      421 aacgtgagcg cgactgatag cttacctttc ttgcggcacg tcccactgcc tcgttgacca
      481 aatgttgacc acgagatctt gaatcgcgtg gtgcgaagtg acgaatcctg aacatgacga
      541 tgcggctaag tggttgttag ggttgagaat tccctgcgtt ttgcagggga ttgtgtgatt
      601 gcccaaaacc cgacttttga gatttgggag catgaggtcg ctggttcgaa tccagtcgcc
      661 ccgactaagg attacgattt cgtcgtcctg ctatcatcga atgcgatcga cgacaccgca
      721 tcattgacct gtccgtactg cttcgaggtt gtcgaggtct agatcgaccc gcagagcgaa
      781 ggtgagtcga ttcgacagtg cgaattggtt gcactgacca gcactgcggg ggcgggcagc
      841 gggccggctc agtagccgga atccatcttg cccttcttct cggccttctg cgcttttcgc
      901 ttgctcttct tgttgcgact ttcgcccgag cgttcttcca ttttgaaatc ctcgggaacc
      961 tcggtgatcg cgccgtcgat gacccaggtg tcggcgtcgg gatagtagac gtacaccgcc
     1021 ttcttgtcgc aaccgtacac ggcgatctga ctgactagct ttcgggcacc ctcggtgtcg
     1081 atgactccga gcttcaactc gttcgggtcg cagaggagat caaacgcggc ccgctctcga
     1141 accgtagtgt tggctcgctt gttgtgcttg cacgcgaccc ccgagatcac gagcagactg
     1201 acgatggcga gacgcaacaa caccacggct cttatgaagc gcccgccggc caggtgccag
     1261 cgcatttttc cttggcctgg ttgccggtcc cgccctgtag cgtcgtaaac cactaattcc
     1321 cgccgcgaat cgatgagagg ccgactcgct ctggaccccg acggccggtg cgctactgta
     1381 acccatcgtg actcgtgcgt gcctacgttc cttcctcatg ctctgcgcgg tcacgacgat
     1441 gtcctgcgca gaaactctgg acatccccgt cgcacctgac ctcgatgacg tggtcgtggc
     1501 ctacgcgagc ccgtcggcta aaatcagtgg caccgtcatg gaggcagtgg gggatgagct
     1561 tctcgagctt cgagagctgc tcaagcagtc cgaggtgttc gaagagatcc tcgacgtcat
     1621 cgtgagcgtc caactcgagc tcgacgcgaa taccgatgag agcggcaacc tcgaggtccc
     1681 cgggctcggc gccttcccag agccgaacgc cgtgatcgaa atcaatcacg actgcgcggg
     1741 gtgggagcgt aacgctgccg agggcgacgc agaaacggcc ggatcgatcc ggctcacgat
     1801 gctgctcgat acaggcgacg tacgccccga ggtctggggc gatgcgaccc agtgcaagtt
     1861 tttggccgcg ctcggcgcta ccacgctgca atcctcgtac gacggccaag tcgcggtcca
     1921 cttcggagaa gagcctgtac caacgggcga gcgcctacgc gacctcgtga tcaccttcgt
     1981 cctcacgggg acattgggag tggcaggcaa ggaagtcccc atcgatcaga gtttccgtct
     2041 tcgtggcgaa ggggacctcg agatcctctg ggagctcaac gacggtacga gcttcgtgta
     2101 tctcttcaac ctcgaaaccc ctcggcaggg tatccgggac gcgaactgta caggagacga
     2161 aggttgcagt tgcagcctcg aggaacgtga atgcaccctt ccctccggaa gcatcttctg
     2221 gtagcggtca tctgtacgat gagcgccggt gcctcggcgg tgccgtcgaa ggcccgcgcg
     2281 tatcacacgt acaaggagcg acttctcgac accacggcgt atagcctcga acgtcgagag
     2341 tttcgcctgg gggcaatgaa gctcagctac gggatcttca aatatctgga gatctcgacg
     2401 tacaccgtgc cctggcttgc gggtctcttt ctcgaggaat ttgagacgat cggttcgccg
     2461 aacgtagagt tcaagtcgac cctaatcaat cggaaccgat ttgcgctcag cgtgtccgtc
     2521 gagtttctgt ggggccgcgt gtcgtcctgc acgaacccaa tgctcaatcc cgacggtcag
     2581 ctttgcacga cggaggcaga gtttagtacg gtcaactggc tcgtttatcc catcagcgtc
     2641 acgtcatcgg tgcgaatcaa ctcgcgtatc agcatccata tcggaggaca gtacaccgcg
     2701 acgcaagcaa ccggtggcac ccgtcccttc gaaggcgata taagtggaag cgccgtcgtc
     2761 aacatgctcc agatctacgg aatgttcgag tggcgcttgt cacgggtagt cgcgctcaca
     2821 gtaacgacgc gatggctccc ttacgtgagc caaacggttg tccgcggcca gatcgtcgtc
     2881 gacgacgaca cgggggccac catcggaatc caggccgaca ttctcgacaa caacgccttc
     2941 gccgtcatcc ccggagcggt cttctcttgg aaccgcgcca acatccgact cggcgtcggc
     3001 tacggggcgt tcttcgtcga gccggtgggc ctggtggtcc cggcttcggt gctgagctcc
     3061 atctcgcccg agttcgacgt gtttgtgcgg ttctgacagg cctgggcgcc tcgagggcgg
     3121 tgatgtctgt cgcctcatcc agctcctcat tcgtaccgtg agaaagatcc taccgtcgtt
     3181 gcggaaactg cggtggtgcg tggcggattg ctctgtagtt ccgtgcgttg gcggccgccg
     3241 gtcgtccgcg tccatgcgcc agtgtgagtc ctctcagtcc atgaaaacag gagataccgt
     3301 ttccgcattc ttttcgcttc cgtcgagccg gtatgactcg accgcgctct cttagcccat
     3361 cgggatcatc gtaaacgccc agttgctcgg tactaggtgt tatgtcgcgt gacttacagc
     3421 gcagacagtg cctttgaatt cgctatgctc tcgaccatgg aacccttgcc ccgattcgtc
     3481 ccgctggaca ggatggttgc cttcagcgac ggcgtgtttg ccgttgccat tacgctgttg
     3541 gtcctcggga tcgagatccc ctcggacgcc gtcctgactg gatctgcgct ggtgatggag
     3601 cgcgaaaagc tcctccacca gctcttcatc tacttcgtcg cgttctggct ggtcgggatg
     3661 tattggtccc aacaaagtct cttcacttcg gggctcaagc agatcgaccg ggggaccttc
     3721 gtcctcaatc tcttactgct cttgcccatc acgctgcttc ccttcgttac ccagttcatg
     3781 ggaaccatgc gcgctgactg gagggccgtt ctgctcttcg ctctaaccaa tgtactcgcg
     3841 acgctcgtgc tcgtgagcat gtggagtcgg atcgcggctc tgcccgaaat tcacaagagc
     3901 cctcagacag cgaccctcgc tgcgaggatg cgggtagagg tccgcgttca cgcggctgtg
     3961 atggtcgtcg gtgttctcgt cgcgcgactc gacgttaggg taggcacgct cgtattcatt
     4021 ctgattccgt tcgcccactt ctacaatttc ctccgcgatc cgtttcgctc tcgcttgggc
     4081 gggctatcct cgggcgaacc caagtgaccc gcgcggcagg atggtcggtc atgtctcctt
     4141 gggctaattg acgctccgac aagcaccgca gccgctcacc gaaagacgag gaagcaaccg
     4201 agcaccatca aagttgggag cagggcgcct gccaaaagtc ccacggcgct gatgcctcct
     4261 tcgaagtgct tgctgaggat cgactgccca gccggattgg gcgcattggc gatcacggtc
     4321 agtccaccac cggtcaccgc gccggcgacc gccgcatatt tcatcccgtc cgtcatcgtt
     4381 ggcaccaagg tgcacaagta agtgactgcg gcgttgtcat tgaacgcggt gagcacagta
     4441 gtgccaagcg ccagttgaat ctcgtctagg ctccccaaca ccggggcgat ccaccactgc
     4501 tgaagcccgc cgtgaatgac gagacctgcc agaaagaagc cgaccaaaac ggcaggccgc
     4561 aagttgaaat ttcgctggaa gtcttcggtg atcttcacga acgcgaggaa aaacaacaac
     4621 acgaagatga gttgcttcgg attgtgagcg tttgcgatgg ccgagagcat gaacagcacg
     4681 tgacccgcgg taacccaaaa cgggatcggc aaatcgtcac ctccctcgtg cttggtcgcg
     4741 gctgggtcat cacgcatccg gttaagctcg tggcgaaaga acagatagta cgcggccgtt
     4801 gtgagccctg tgccgagcgc tgccttccag ccaaaatggc tcatcatgtg gccggtcgtc
     4861 cagccccagg cgtcggccac catcaggacc ggcggagccg cgaagttcgt ccaagtgccg
     4921 cccaccgaga tcgcgacgaa caggaggcca agcgtgccat aggcgagcgg cttgctgggc
     4981 ttgtgcgcgt agaacttgct actgaggatg gacgcacaaa tcgtcatggc ggccggctcg
     5041 gtgatgaacg atcccagtaa cggacctacc gctaccagga ccagccacca ggcggcaggc
     5101 gtttcaccgc cgagacgagc aagcaggcgc agcgctcttt cggtcagctc cagaatgggc
     5161 cgagtcgagg caaaggtcat gatcaccacc acgaatacgg gctcggtgaa gctcactcct
     5221 tcgttgatgt aagcaaccgc ggtggcccga tcgtagaaga ccacgatcgt atagaacaaa
     5281 gggatgcccc agattccgaa aaccgcctcg gcatcgccga gaaagtcaaa gacacgcgcg
     5341 ctcgccgaga cgcgagtttg agtcgcccca gttcgaacct tctcttcatg gcgccgctcg
     5401 agcacacgag caatgttccg aaaccgatac gttaagaagg tgtgaacgat ggcgccaatg
     5461 aatatcagcg tcgcccacag attgaagggc accacctcga ttcgatgccg caggatggcc
     5521 atcacgctcg tgaggtcggc atcaccgtag tcttcgagcg cggggggaaa gtcaattccg
     5581 ggtccggtgt gccccgagga cgccgctgct aacgccggac aagcagcgag catcaataga
     5641 gcagtcaccc ccacaacttt gcccaggccg atgatgagac gcgacacagg acccctccaa
     5701 gaccgcatag tgcaactgta gcagacgccg gtaaccgaca atttcgatcg atggccgtcg
     5761 ccgtcgacgg gcatctcagt ttgcttctct cagctgaacc agcctcgctt gtagaagaac
     5821 caggcggagc ccaccccgag ggtcgccatg gtggcaagag cgattcgata gccgacgggg
     5881 tctgaaagca gaggcatctc ctcgaagttc attccccaga tacccgctag gagcgtcagc
     5941 ggaagaaaga tcgcggagac gatcgtgagc agcccgagcc gttggttgag atgctcttgt
     6001 ttctggtcct gtatccgacg aaggagattt tcgacgcgcc cgtccatccg ctcgttcagg
     6061 cgaaagcttg cgctggcagt cgcgcgaaga agacgcaagg gcgcctggag agaggagagg
     6121 tcaagccctt tgctttgcgc ctgctcgatg agctcgaacg cttcctcctg ctcttcggca
     6181 acttcatcgg tgagcatgac ggagcgcttc aatcgctcga gctcgagagg atcgacggcg
     6241 ccaatgtctt cgtccatcag ctgacccagc tctagcagtc gatcacgaat cgctctggtg
     6301 gtgcgggccg tccttgctcc gtggcgcaaa agaatcgcgc acaacacccc cgcagtggaa
     6361 actcgttcga gctcgaggaa cccgaccgcg cggttcatgt cctgcgtgcc ctcgatgggt
     6421 tccgcgtgca tcgtcaccaa gaggttctcg aggcaaagcg cggatgcgtg accccgcttg
     6481 gtgcattgcc gatcgagaaa catggtccac tcggcaaacg ttccctcggg caaagcaaac
     6541 gcggacgtgc ctcgcgcctg ctctagacat cgctgtctta gaaatccagt ggccccgacc
     6601 tcctcgagca agccctcgag cgactcgcgg tcgtactcgg atacatcgag ccagaacacg
     6661 ccgtcgccag cacgccaacg atcgagggcc tcgaccttcg aggttgccgc gagcgttgga
     6721 ccagcgtcga gtcggtagca ggagaccttc atcggggagc gcaaccctac agtctgtgtt
     6781 gcaccgtgaa aaggggatcg ggatgtttcg ctactgtttc gtccgttcca caacctgcag
     6841 cgtggggaag gatctcgact cgcatcatcg ctgccttctt gcgggcagga acgcacagcg
     6901 tatgcgccgc ggccggatgc aggactcggg acctacaccg aagcgcagag cgcgacccag
     6961 aatgcgcggt ccgcttgggt caacttgggg cgtttggtct tgcgcttcag actggccagc
     7021 tgctgtcgca aagcgaggtt ctcgagagcg agctagcgtt gcggccgtag gctcgatcgg
     7081 gcgatcgcga gtagggcgta gagcaaccga agcatggggg caggttgggt tgacccgagc
     7141 ttggaaacat cgacgattgt gcgcgcggcg gggttctcag gaggcacagg gagctcgata
     7201 ggctcgtcta gtgctcggac ggatctccgg aatgatagta gtccgtgacg aagacctcca
     7261 caccgcccgc tggaacagag tagccgtaca cccgaatctc caaacacgcc tgatcgattg
     7321 ccggactctc taagacgacg tggcgctcga gccccggatt aaggtccgcc gcgatcagcc
     7381 ttggacggtt gcaggtattc caatagctaa tgagcaggta tgggattgtg gaaaggtccg
     7441 gccagtcgat gtacatcccc atcttccact gcgtcacgtc tggctcgagc gcgtctccct
     7501 tcgtatccgc agcgtggaaa agtacgcgct cgttttcgtg gatccggaac gatcggtgag
     7561 caagcccttt tgggctttgc atcgcgtcaa acgggtgcat cttcactctt ccggtgcccc
     7621 aaacactcga cgtgccgaca gcgacaccag gctgcgaaac atcggcgcct gagccgtcgc
     7681 ccattgtgag gacgtgagac ttgagcattc tcgcgtccca cacggcaggg ccgaactctt
     7741 cacggacgag gcctgcggct gcggaaatga ccggcgctga gaaagaggtt ccgctccggc
     7801 cgacctcatc gtaagtgtcg tcgttgttga aatacgcgca caagttcccc ggggcagcaa
     7861 tggataccac cggaatgtca acgccactcg gatagtccgc tgagtgttgc ccgcccacac
     7921 ccacgcgagc aaagcctcgc gagctatttt cggcgatctc agcgaggtcg taatcgatgc
     7981 cttgcgacgt ttccacattg ccgacggcca gcacttcagg cctaacggcc gggtagcata
     8041 cgctgcatcc ggaggtgggt tcacaacgac cttgctcgga gttctcgttc ccggccgcgg
     8101 caacgacgag cgcaccgccc ttcgtgaccg ttcggatgac ttgattgagc ccgccacaat
     8161 ctgctgtcag atctgtcaga tcgaatgcgc actctgtcac ggcgagcgac aagttgatta
     8221 catctgcgcc caaaagaaaa gcagtcgcga ccgcagcagc cacgaagccg gttgagctgg
     8281 cgttcagata gaggattgag gcttcgggtg cgatacccgt tcttcttcgc tgttcatcgg
     8341 ggtcggtggt tcgtggatct tggccctggg tgaggtcccc cgccgcgatg gacgttaccc
     8401 atgttccgtg gttcgccaac gtttgtagat ctccggtcca cggcaggcac gcatcgagat
     8461 cctcactcac gaacgaacaa ttcagctcag cgcgaactcg cgattcacac gatccaaaca
     8521 cgcagtctcc ccagccaggg tggacgcggt tgagtcggtt caaaacgaac gagctaatcg
     8581 atgcctcgac aactgcgatc ttgatgtctt catagcctgc tgcaggctcg cagattctgg
     8641 atttggattc gcccttgatc tcctgatccc agtactccga caagaaagtc gcctttctca
     8701 cctcttcttg atcgtacaat agaatgatgg gttgccaggc cggatggatc gctttcacat
     8761 caggatgtgc agcgacatcg gcaatggcat cgggagcaat ccaggcctgc agatggttga
     8821 ggagcccaac aggtttcgtt gttcgtgcac cgagttgatc gaggtaggcc ttgatgcctt
     8881 gctgttcgcg ttcaatgagt gccttgcgat ccgcaaggac acgatcgcgc gatgcggcgt
     8941 ccatggcctg caattgaggc agttccggaa aagggagatc gcccatgtcg agtacgactt
     9001 cgattggctc gtcggtcgca cgattctccg cgtactccaa gacgtatgcc tgaacgcgct
     9061 cgatactttc cgcttgggtt gctggagtag gctcctcgga tgcggtggca cgggcggcgg
     9121 gcatgctcgc gccaaaggac gagagctcct caaggaaatt cttgacttcg acttgactga
     9181 ccgtaacgtc cgagtcccca gggtcgagga cgtagtcctc gctccagtct tcgcaagact
     9241 gcactccgtc agagtgaatc gccggcccac aatctcccag atcttcgtcg catggactca
     9301 cttgaaataa gacgaggaac cccaagaccc ctaagccgac ccagcgcgtc ttcgtatcca
     9361 tcctgcttga ccgtacaccc tgagcgctcc tggaggaaga tccgcccggg ttggcgagca
     9421 ggaacgaaga agatctagac ttccctggaa ttcgtggaca tctcctggcg cagtgttgtg
     9481 ccttctcggg tgtccacaa
//
LOCUS       DASRZF010000058         2323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152 k141_41002,
            whole genome shotgun sequence.
ACCESSION   DASRZF010000058 DASRZF010000000
VERSION     DASRZF010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41002"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VFG22_02695"
     CDS             <1..212
                     /locus_tag="VFG22_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Zn-binding domain-containing protein"
                     /protein_id="HET6415178.1"
                     /translation="AGGIGLASRLFEEREELLRRARRLIESCGCDEGCPGCIGPDASG
                     ADEPEAARKRLVLGLLDAVGVSATH"
     gene            219..1397
                     /locus_tag="VFG22_02700"
     CDS             219..1397
                     /locus_tag="VFG22_02700"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HET6415179.1"
                     /translation="MSIKRNLARLSQSTPRHLDVASIRGALPGQRRETEHGELHLIDA
                     YLEPRHHHGRAPIAKALQVSTERLAQLALDPLLAEVDLQRALFLDTETTGLAGGAGTV
                     PFLIGIAWFEDQSMRIQQLFLSDLGREAPMLHWLRERVQEASCVVSFNGKTFDWPLLR
                     NRFVLNRVRAPALPPHLDLLHCARRILRPRLKSVRLVELERRVLGMHREDDVSGALIP
                     QLYFDYLDGGDASPIAKVIEHNANDLIALAALVAELVSHFDEVHGGDDPRDHLAYAKV
                     AERTGDPSRARSFADAAAQGGGESACTVQACMLNARIARRGGEADDEERALLEALRAA
                     DDEELRAAVQLALSKFYEHRRKDITRALEHAAGTGLAEGEEAAERRVARLARRLASQS
                     "
     gene            1545..>2323
                     /locus_tag="VFG22_02705"
     CDS             1545..>2323
                     /locus_tag="VFG22_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415180.1"
                     /translation="MNRFWLAFGVLGAVTMAVPGCGESSSGDVGPLEGFVCGKDGNTY
                     SVEDAVRGEHGVEHRGRCDAPMHCGSPEDCFVGDQCMPADADGNWCIPGPTRCNCPGV
                     FEPVCGGDSRNYINACEAACAGVGIRHEGYCEGPPGEGCVRAGCSDQLCVEEGMDIAS
                     TCEWRPVYECYQQATCERQENGQCGFTPTHELVECLDRFGEGMCRIDPRWQDGFEAEC
                     ESPQVPGIVGIIAVEPHQMMQLGEWFGASLQLLEPPVFGDL"
BASE COUNT          400 a          678 c          772 g          473 t
ORIGIN      
        1 tggcgggtgg aattggcttg gcgtcacggc tcttcgagga gcgcgaagag ttgctgcggc
       61 gggcgcgccg tttgatcgag agttgcggat gtgacgaggg ctgtcccggt tgcatcggac
      121 cggacgcttc gggcgccgat gagcccgagg cggcgcggaa gcgcctcgtg cttggtttgc
      181 tcgatgctgt cggcgtgagc gctacccact gaggcccgat gtcgatcaaa cgaaacctcg
      241 cccgcctctc gcaatctacg cctcggcatc tcgatgtcgc gagcattcgg ggcgcgctcc
      301 caggccagag gcgcgaaacc gagcacggag agctacacct gatcgatgct tacttggaac
      361 cacgtcatca ccacgggcga gcgccgatcg ccaaggcgct ccaggtctct accgagcgac
      421 tggctcaact cgcgctcgat ccattgcttg ccgaggtcga tcttcagcgc gccttgtttc
      481 tcgacaccga aaccaccggg ctcgccggtg gagccgggac ggtgccgttc ctgattggca
      541 tcgcctggtt cgaggaccag tccatgcgaa ttcagcagct ttttttgtct gatctgggtc
      601 gtgaagcgcc gatgctccac tggctcaggg agcgcgtgca agaggcctct tgtgtcgttt
      661 cattcaatgg caagactttc gattggccgc tcctccgcaa tcgattcgtc ttgaatcgtg
      721 tccgcgcacc ggctttgcct ccacacctgg atctcttgca ctgtgcgcgt cgaatcctac
      781 gaccgcgctt gaaatcggtt cgactcgtgg agctggagcg gcgcgtgctc gggatgcacc
      841 gcgaagacga cgtttcgggg gcgctgatcc ctcagctcta cttcgactat ctcgacggcg
      901 gggacgcatc tccgatcgcc aaggtgatcg agcacaatgc gaatgatcta atcgcattgg
      961 cggcgctcgt tgccgagctg gtgagtcatt tcgacgaagt gcatggcggt gatgacccgc
     1021 gcgaccacct cgcctatgcc aaagttgcgg agcgaaccgg ggatccatcg cgcgcacgct
     1081 cgtttgcgga tgctgctgcg cagggaggcg gtgagtcagc gtgcaccgtc caagcctgca
     1141 tgctcaatgc gcgtatcgct cgccgtgggg gggaggccga cgatgaagag cgcgcgcttt
     1201 tggaagccct tcgcgccgcg gatgacgaag agcttcgcgc cgcagtgcaa ctcgcgttga
     1261 gcaagttcta cgagcaccgg cgaaaagaca tcacgcgcgc gctcgagcat gccgctggga
     1321 cggggcttgc cgaaggcgag gaggccgccg aacgtcgtgt ggcccggctc gcccgtcgct
     1381 tggcaagcca gagctgacct ttttgcactg gaatccggcg agcttggcac ggcctggcac
     1441 agcgggttat tctcgaccgg ccagttttac cgcgtttttg agcgcccgac ctggcatcaa
     1501 agttgctttt gtagacggcg tcccccttgc cgtggagtga agcgatgaat cgattttggt
     1561 tggcttttgg agtcctaggc gccgtgacca tggcggtgcc tggttgtggt gagagcagca
     1621 gcggcgacgt tggaccgctc gagggcttcg tctgtggcaa agacgggaac acgtattccg
     1681 tcgaagatgc ggttcggggg gagcatggcg ttgaacatcg agggcgctgc gacgcgccga
     1741 tgcactgcgg ctcacccgag gattgcttcg taggcgatca atgcatgccg gctgacgcag
     1801 acggcaattg gtgtattccc ggtcccacgc gctgcaattg ccccggcgtc ttcgagccgg
     1861 tctgtggtgg agactcgcga aactacatca atgcatgcga ggccgcctgt gccggtgtcg
     1921 ggattcggca cgagggatac tgcgaggggc cgccgggaga gggctgtgtc cgcgctggct
     1981 gtagcgatca gctttgcgtt gaagagggca tggacattgc gagcacctgc gagtggcgcc
     2041 ccgtatacga gtgctaccag caagcaacct gtgagcgtca ggaaaacggc caatgcgggt
     2101 tcacgcccac ccacgagctc gtcgagtgtc tcgaccgctt cggtgagggc atgtgtcgca
     2161 tcgatccgcg ttggcaggat ggattcgaag cggaatgcga gagtcctcaa gtgcctggca
     2221 tcgtcggcat catcgccgtg gagcctcatc aaatgatgca gctcggcgaa tggtttggtg
     2281 caagcttgca gctgctcgaa ccgcctgtct ttggcgatct gta
//
LOCUS       DASRZF010000059         7871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3481762, whole genome shotgun sequence.
ACCESSION   DASRZF010000059 DASRZF010000000
VERSION     DASRZF010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 7871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7871
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3481762"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..941)
                     /gene="panC"
                     /locus_tag="VFG22_02710"
     CDS             complement(84..941)
                     /gene="panC"
                     /locus_tag="VFG22_02710"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HET6415181.1"
                     /translation="MTELVRKPAEFRRACEDARSRSARVGLVPTMGALHEGHFSLMDE
                     ARKRSDFLVVTIFVNPLQFAEGEDLERYPRTLESDLAGCQKRQVDLVFAPESGSMYPD
                     GFQTGVSVSEVSRPLEGEFRPAHFDGVTTVVAKLFNLTGPCTAVFGRKDFQQWRVLSR
                     MARDLDMPVEVLGCPIVREPDGLALSSRNRYLSSSERERALGIVSGLRAAHDAWQNGE
                     RNVERLRTLTEEPVRSRFERVDYVAVVDPETLLPPVDPASRLLVAVAAHLGATRLIDN
                     VVLGEDPRP"
     gene            complement(938..1843)
                     /gene="panB"
                     /locus_tag="VFG22_02715"
     CDS             complement(938..1843)
                     /gene="panB"
                     /locus_tag="VFG22_02715"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HET6415182.1"
                     /translation="MSGSGHASEQRPVTVPRLRKMKSEGTRITMVTAYDATFARLFDE
                     AGMDVLLVGDSLGMVVQGRSSTLPVTVDEVIYHCRAVARGTRRAHIVGDMPFLSWQLS
                     PEDALRNAGRFLSEGGAQAVKLEGGVDAAPTIQRIVNAGIPVMGHVGLTPQSVHAMGG
                     FRVQGKTEEAAARVLADAIAVAEAGAYSLVLEGIPTDLAKRITESIDIPTIGIGAGPH
                     CDGQVLVCYDLLGLTADMKPKFVKRYAEFFEDGLAAARLYRDEVRAGVFPSEAYSFGN
                     VKADDATASSLSLVRAAHPAYGPKG"
     gene            complement(1969..3213)
                     /locus_tag="VFG22_02720"
     CDS             complement(1969..3213)
                     /locus_tag="VFG22_02720"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6415183.1"
                     /translation="MSLLKKLFSSDPLALERKADALYENGDYGPAKLAYEKALGASPE
                     SERPAIAAKARRCTDRIAEERIEEAKAYLAQGSIELAEQELEGALEVAHDEGLRDEAQ
                     ALLDGLEAKEAQEQAAAPEMTDEDRVALLMGQWLEAQAEEYEGYGDALLDALLAMQKE
                     QVDRARTELEALLSDAPFPRYLWLEVGRARLLCEDTSGGKEALQRFLAALEDDEGGET
                     KLAANLALARLADDAGQFEEAMRHFEAAVHALPEDYRPYLAMGAFLREKGHASEALEV
                     LQTSLTLSQTSGTDWRLFEELGLASDLAEKPDDAVKFLEQVVEFFANHQISDFPTGTA
                     TTLAKLYESEGRLDRAADMYRALSEGSDRERHGLYHYEAGRLLLSLGLDDEARRMLTR
                     AEALGSEADPELAGRIAALLRA"
     gene            complement(3217..4326)
                     /locus_tag="VFG22_02725"
     CDS             complement(3217..4326)
                     /locus_tag="VFG22_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415184.1"
                     /translation="MLAAPTLPVPTEEVPQGLRRFVDAAAPGAAKTMAARGMVPVKGG
                     DLVFLLVQLSVDADPVVSKSANETLDGLPDNVLLPACEGELHPAVFHEIALRFSTNDD
                     VLERLASNHGVSDATIARISAHCPERITEIIAVNQQRLLGAPLIIEALYKNRNTRMST
                     ADRLIELAARHDVELTGIPMFEELKKAVQGQLIPEPSDEPLPGDAAFSNALEKDADQD
                     AVARDDVDGKEETKKEFVPLQSALRDMNVGEKIRMALLGNAAARSILVRDPNRVVCMA
                     AIASPAMSESEANNAAQSREVSEDVLRYIGRKREWLRSYEIKRNLCNNPKTPLALSMT
                     FLNHLRRNDLKALSRSRGIPGPLKQAAKRRSKQSE"
     gene            complement(4353..5138)
                     /locus_tag="VFG22_02730"
     CDS             complement(4353..5138)
                     /locus_tag="VFG22_02730"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET6415185.1"
                     /translation="MSDRAVLYEAADRIGTITLNRPDHRNAMTPELLDAFSEAIDEAR
                     ADGEIRCLVMTGKGRCFSAGADLRSSMQRSDLGKPSREASFAMYEPFLRVLDVEVPVI
                     AAMNGHTVGGGFGLSLLADIRVANVDAKYGVNFARLGIHSGLGISYLLPRLVGAAYAS
                     ELLFTGKLIRGDEALRIGLVTHADEADEVLPTAMGLARAIAASAPMAVQQMKASIRRG
                     LRWEIREAALEEAGLQAASLETADAKEGVSAILEKREPEFMGR"
     gene            5234..6025
                     /gene="ftsE"
                     /locus_tag="VFG22_02735"
     CDS             5234..6025
                     /gene="ftsE"
                     /locus_tag="VFG22_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HET6415186.1"
                     /translation="MQGTKVVRPFPFFRAGARASQPQLSKPILVLEDVYKFFRPDQPT
                     LRGVDLSIERGEFVFITGPSGAGKSTLLKLVYRELMVDEGRVLFCGRDIGRLTDKSIP
                     FLRRNLGVVFQDFRIIDHWTVFENVAVALEIVSMPQRLIRARVAEALERVGLAGRGPE
                     QTSNLSGGEKQRVAVARAIVPEPALILADEPTGNLDPHLAIDILTLFEEINSTGTTVL
                     FATHDHSLISKRDHRLISIDEGRVSEAQKGLKHWQGSSGIHTLVG"
     gene            6000..6911
                     /locus_tag="VFG22_02740"
     CDS             6000..6911
                     /locus_tag="VFG22_02740"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HET6415187.1"
                     /translation="MEFTRWWGETMDVKNAITRARRGLREERRLYIVAVTSLAVAFIC
                     LGATLLGVTNLDAAATRWGQSGRITLFLKDDADAQDIAQLRVVLEGLADVREVTHVTP
                     AQARQSFLEEADVGADLSALPSEVFPASLEVALVQGVARERSESIASRLAQFRAVSDV
                     ESYGSWFQKLDHLLWGARMLALGLALLVMFCVVAVIGNTIRLAVARRRQEIEVLKLCG
                     ATNGFVRGPFVLEGIFQGFASALLAVLALLVGYLALHESVDSTLAALTGSRVVFLSAW
                     TLVALLAGGALIGAIGSAVSVRRYLSV"
     gene            6913..>7871
                     /locus_tag="VFG22_02745"
     CDS             6913..>7871
                     /locus_tag="VFG22_02745"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HET6415188.1"
                     /translation="MKIKSRFWVWIAAGSLGAGLAAGATAEQDLGVSALTDVGTVEGE
                     IAESLARVEHAQTRQAQIDTEISGLADKRDEAEARLHDRARALYRIRRSGMLPVAGGF
                     DALMAHLARVQRLSRMVKGDLDTMDFLGQRGAALRVEKNQLAEALREAQVELQALEAK
                     KVELEQSSRSASLFQDTLRGEREETPSDDMGYGRIRVSGDMDDLGEGFAEMRGQLALP
                     VQGSMRIGEASREGAPGLEFYGRAGGPVRAAADGRVAFARNYGAYGLMVIIDHGNSFY
                     TVYGGLGSTDVQTGDWVGMSTQIGTLDMGSPEAILFFEVRRGTR"
BASE COUNT         1336 a         2478 c         2550 g         1507 t
ORIGIN      
        1 ccctggttac tggccgcagg gattgcactc gcggcgaccg tcaccgtcgc ttggttccta
       61 tacgggctct gaggccctga gtctcaaggc ctggggtctt ccccgagcac cacgttgtcg
      121 atcaagcgcg tggccccgag gtgtgcggcg acggcgacga gtaaacgact cgcagggtcc
      181 accggtggga gcagggtctc ggggtcgacg accgcgacgt agtcgacgcg ctcgaatcgc
      241 gaccggaccg gttcttcggt caaagtgcga agccgttcca catttcgctc gccgttttgc
      301 caggcatcat gcgccgcccg aagcccggag acgattccta gcgcccgctc gcgctccgat
      361 gagctgaggt accgattccg tgaagagaga gccagcccgt cgggctcgcg aacgatgggg
      421 cagccgagga cctcgaccgg catgtcgagg tctcgcgcca ttctcgagag cactctccat
      481 tgctggaaat ccttgcgccc gaacaccgcc gtgcacgggc ccgtgagatt gaagagctta
      541 gccaccacgg tagtcacccc gtcgaagtgc gcagggcgaa actcaccttc gaggggccgg
      601 ctgacctccg agaccgatac gccggtctga aatccgtccg gatacatcga gccgctctcc
      661 ggagcgaaga cgagatcgac ctgccgcttt tggcaacccg ccaaatcgct ttcgagggtt
      721 cgcgggtagc gttcgaggtc ctcgccttcg gcaaactgaa gtggattgac gaagatggtc
      781 acgacgagga aatcggagcg cttcctggct tcgtccatga gcgagaagtg cccttcgtga
      841 agcgcgccca tcgtcggcac gagcccgact cgcgccgaac ggcttcgcgc gtcctcgcag
      901 gcccgccgaa actcggcagg cttgcgcacg agctctgtca tcccttgggt ccgtaggcgg
      961 ggtgcgcagc tcgaacgagg ctcaaactgc tcgccgtggc gtcgtctgcc ttcacgttgc
     1021 cgaagctata ggcctcgctt ggaaaaacgc cggcgcgtac ctcgtcccgg tagaggcgcg
     1081 ctgctgccag gccgtcttcg aagaactccg cgtaacgctt tacgaactta ggcttcatgt
     1141 cggccgtgag gcccagcagg tcataacaaa cgagcacctg tccgtcgcag tgaggccccg
     1201 cgccgatccc gatggtcgga atatcgatgc tctcggtgat gcgctttgcg agatccgttg
     1261 ggatgccttc gagcacgagg gagtaggcgc ctgcttcggc gacggcgatc gcgtccgcca
     1321 gaacccgagc ggccgcttct tcggtcttcc cctgcacccg gaatccgccc atcgcatgca
     1381 cgctctgtgg agtgagaccc acgtgaccca tgacgggaat ccctgcgttc acgatccgct
     1441 gaatcgtggg cgcagcatcg accccgccct cgagcttgac cgcttgtgcg ccgccttcgg
     1501 agagaaagcg accggcattg cgaagcgcgt cctccggact cagctgccaa ctgagaaagg
     1561 gcatgtcacc gacgatgtgc gcgcgtcgtg ttccgcgcgc gactgcgcgg cagtggtaga
     1621 tgacctcgtc gaccgtgacc ggaagcgtgg aactccggcc ctggacgacc attcccaggg
     1681 agtcgccgac gaggaggaca tccatgcctg cctcgtcgaa gagacgggca aacgtggcgt
     1741 cgtacgccgt caccatggtg atccgcgtgc cttcggactt catcttgcga agacgcggca
     1801 ccgtcaccgg ccgctgctcc gacgcgtgtc ctgatccgct catgggtttg actcgctttg
     1861 gtcgcggcca ggtcgattcc cggtccacgc cgctacaccg ctaaagatag caccgattcc
     1921 caaaccgtca ttggggcaaa aagcagggca aaatcagcgc actgggggct acgctcgcaa
     1981 gagcgccgcg attcgtccgg caagctcggg atccgcttcg gagccgagcg cctcggctcg
     2041 ggtgagcatg cgccgggctt catcgtccag cccgaggctc aagaggagcc gccctgcctc
     2101 gtagtgatag agcccgtgcc gctcccggtc gctgccttcg ctcagcgccc gatacatgtc
     2161 ggcggcgcgg tccagtcgcc cctccgactc gtagagtttc gccagcgtag tcgccgtccc
     2221 agttgggaag tcgctgatct ggtggttcgc aaagaactcg accacttgct cgagaaactt
     2281 cacggcatcg tcgggtttct ctgcaagatc ggatgccagg cccagctcct cgaagagtcg
     2341 ccaatcggtg cccgaggtct ggctgagtgt gagcgaggtc tgaagaacct cgagcgcctc
     2401 tgaagcgtgc cccttctccc tcaggaaggc ccccattgcg aggtaggggc gatagtcctc
     2461 gggcagcgcg tgtactgcgg cttcgaagtg cctcatcgcc tcttcgaact ggccggcgtc
     2521 atcggcgagc cgcgccagcg cgaggttggc ggccagcttc gtctcgcccc cctcgtcatc
     2581 ctcgagcgcc gcgagaaagc gctgcaaggc ctccttgccg ccgctcgtat cctcgcaaag
     2641 caggcgtgcg cggccgacct ccagccagag gtaccgagga aagggagcat cgctgagcaa
     2701 ggcttcgagc tcggtgcgcg cacgatcgac ctgctctttt tgcatggcga gcagcgcgtc
     2761 gaggagtgca tcgccgtagc cctcgtactc ttcggcctgg gcttctagcc actgtcccat
     2821 cagcagcgcg acccgatctt cgtcggtcat ctccggggct gcggcctgtt cctgtgcctc
     2881 cttggcttcg aggccgtcca gcaaggcctg cgcttcatcg cgaagcccct cgtcgtgagc
     2941 gacttcgagc gcaccttcga gctcttgttc tgcaagctcg atcgagccct gagcgagata
     3001 cgccttcgcc tcttcgatgc gctcttccgc gatccggtct gtgcagcggc gcgccttggc
     3061 ggcgatggcc ggccgctcac tctccggaga cgcgccgagt gccttctcgt atgcgagctt
     3121 tgcgggaccg tagtcgccgt tctcgtagag cgcgtcggcc ttcctttcga gcgccagagg
     3181 gtctgaggaa aacaatttct tcaacaggct catggcttac tcggactgct tggaccgtcg
     3241 cttggcggcc tgtttgagag gcccagggat gccgcggctt cgcgacaagg ccttgaggtc
     3301 attacggcgg agatgattga ggaacgtcat cgaaagtgcg agcggtgtct tcgggttgtt
     3361 gcagaggtta cgtttgattt cgtagctccg aagccactct cgctttcttc cgatgtagcg
     3421 aaggacgtcc tcggaaacct cgcggctctg cgcggcattg ttcgcttcgc tctcgctcat
     3481 cgcgggggag gcaatcgcgg ccatgcaaac gacccggttc gggtctcgca ccagaatcga
     3541 acgagccgcc gcgttgccga gtagggccat tcgaattttc tcgccgacgt tcatgtcgcg
     3601 aagcgcagac tgaagcggca cgaactcttt tttcgtctcc tctttgccgt cgacgtcatc
     3661 ccgggcgacc gcgtcctgat cggcgtcctt ttcgagagcg ttggaaaacg ccgcgtcccc
     3721 cggcagcggt tcgtcgctcg gctccgggat gagctgaccc tgaaccgcct tcttgagctc
     3781 ttcgaacatc ggtatgcccg tcagctcgac gtcgtggcgg gccgccaact cgatcaggcg
     3841 gtcggcggtc gacatccggg tgtttcggtt cttgtagagc gcctcgatga tcagcggtgc
     3901 acccaggagc cgctgctggt tcaccgcgat gatttcggtg atgcgctcgg ggcagtgtgc
     3961 ggagattcta gcgatggtcg catcggaaac cccatggttg gacgcgagcc tctcgagaac
     4021 gtcgtcgttc gtcgagaatc gaagcgcgat ttcgtggaaa accgccggat gaagctcacc
     4081 ttcgcaggcg gggagaagga cgttgtcggg caatccgtcg agcgtttcgt tggccgactt
     4141 ggacaccacc ggatccgcgt cgacgctcag ctggacgagc aagaagacga ggtcgccgcc
     4201 tttgaccggc accatcccgc gggccgccat cgtcttcgcg gcgcccgggg cggccgcatc
     4261 gacgaagcgt ctcagtcctt gggggacctc ttctgttgga acgggtagcg tgggggcggc
     4321 gagcatttgt tcttgcaata ctacgctagg agctatcggc ccatgaactc gggttctcgc
     4381 ttctcgagga tcgcgctcac gccttctttg gcgtccgcgg tttctaggct tgccgcttga
     4441 agcccagctt cctctagagc ggcttcgcgg atctcccagc ggagcccacg ccggatcgat
     4501 gctttcatct gctgcacggc cattggagca gacgccgcga tggcgcgagc gagccccatc
     4561 gccgtgggaa gaacctcgtc ggcttcgtcc gcgtgggtca cgagcccgat gcgaagagcc
     4621 tcgtctcccc gaatcagctt gcccgtgaac agcagctccg aggcgtatgc cgcgccgacg
     4681 agtctcggga gcaggtaact aatgccaagt ccagaatgga tccccaagcg tgcgaaattc
     4741 actccgtatt tggcgtcgac gttggcgacc cggatgtcgg cgagcaggct gaggccaaag
     4801 ccgcctccca cggtgtgacc gttcatggcg gcgatgacgg gcacctctac gtccagcacc
     4861 ctcagaaacg gctcgtacat cgcaaacgag gcctcgcggg acggcttgcc cagatcgctg
     4921 cgctgcatcg aggagcggag atcggcgccc gcgctaaaac agcgaccctt gcccgtcatc
     4981 accagacaac ggatctcgcc gtcggcgcgt gcttcgtcta tcgcctcgga aaacgcgtcc
     5041 aaaagctcgg gagtcatggc atttcggtgg tcgggacgat tcaaggtgat cgtcccgatc
     5101 cggtccgccg cctcgtacaa gaccgctcgg tcgctcatcg tcggaggact ctagccggac
     5161 ggcggccctc ggcaaccgcg gattgttggc caatttcttg cccaggagcg actgaacggg
     5221 gtagcgggat tctatgcagg gaaccaaggt tgttcgcccg tttccgttct ttcgcgccgg
     5281 ggcacgcgcg agccagccgc agctctcgaa gccgattctg gtgctggagg acgtctacaa
     5341 gtttttccga cccgatcagc cgacgctgcg cggagtcgat ctgtcgatcg agcgcggaga
     5401 gttcgtgttc atcacgggcc cgagcggggc tggaaagagc acgctcctca agctggtcta
     5461 ccgggagctc atggtcgacg agggtcgcgt gctgttttgc ggccgtgaca tcggtcgcct
     5521 gaccgacaag tcgattccct ttctgcgtcg caacctcggc gtcgtgtttc aggatttccg
     5581 aatcatcgac cactggacgg tgtttgagaa cgtcgccgtg gctctcgaga tcgtgtcgat
     5641 gcctcagcgg ctcatccgag cgcgcgttgc cgaggctctc gagcgcgtgg gtctcgcagg
     5701 gcggggtcca gagcagacca gcaatctctc cggcggagag aagcaacgtg tcgcggtcgc
     5761 ccgagcgatc gtgccggagc cggcgttgat cctcgccgac gagcctaccg gcaatctaga
     5821 cccgcatctc gcgatcgaca ttctcacgct cttcgaagag atcaactcga ccggaacgac
     5881 cgtgctcttc gctacacacg accattccct gatctccaag cgagatcacc gcttgatttc
     5941 aattgatgaa gggcgggtct ccgaggctca gaagggtctc aagcattggc agggcagcag
     6001 tggaattcac acgctggtgg ggtgaaacga tggacgtgaa gaacgcaatc acacgcgcga
     6061 ggcgaggcct ccgcgaggaa cgacggcttt acatcgtggc ggtcacgagc ttggccgtcg
     6121 cgttcatttg cctgggcgcc acgcttctcg gcgtcaccaa tctcgacgcc gccgcgacac
     6181 gctggggcca gagcggccgc atcacactct tcttgaagga tgacgcggat gctcaggaca
     6241 tcgcgcagct tcgggtcgtg ctcgaagggc ttgctgacgt gcgcgaggtc acgcatgtga
     6301 cgccggcgca agcccgccaa tcgtttctgg aagaggcgga cgtcggggcc gacctgagcg
     6361 cgctgccctc cgaagtcttt cccgcgtcgc tcgaggtggc gctggttcaa ggtgtcgcgc
     6421 gtgagcgcag cgaatccatc gccagtcggc tggcccagtt ccgggccgtg agcgacgtcg
     6481 agagctacgg aagctggttc cagaagctcg accatttgct ttggggcgct cgcatgctgg
     6541 cgctcggact cgccttgcta gtgatgttct gtgtggtggc ggtgatcggg aacacgattc
     6601 gattggcggt cgcgcggcgg cgccaggaga tcgaggttct gaagctctgt ggagccacca
     6661 acggcttcgt tcgcgggcca ttcgtgctcg aaggcatctt ccaagggttc gcttcggcgc
     6721 tcttggcggt gctcgcgctg ctggtgggat acctggcgct gcacgagtcc gtcgactcga
     6781 ctctggcagc cttgaccggc agccgcgtcg tgtttctgag cgcatggacc ctcgtggcgc
     6841 ttcttgcggg cggcgcgttg atcggtgcaa tcggcagcgc ggtctcggtg cgccgctatc
     6901 tctcggtgtg aaatgaaaat caaaagtaga ttctgggttt ggattgcagc ggggagtctc
     6961 ggagccggtc tggcggccgg agccaccgcg gagcaggacc tcggggtcag cgcgctcacc
     7021 gacgtgggga cggtggaggg cgagatcgcc gaaagcctcg cccgcgtgga acatgcgcaa
     7081 acacgccaag cccaaatcga caccgagatc tcgggcctcg cggacaagcg cgatgaagcg
     7141 gaggcgcgcc tgcatgatcg ggcacgggct ctctatcgaa ttcggcgttc cgggatgttg
     7201 ccggttgcgg gcggctttga tgcgttgatg gcgcacctcg cgcgcgtgca gcgtctctcc
     7261 cgaatggtga agggcgatct ggatacgatg gatttcctcg gccagcgtgg cgcagcccta
     7321 cgggtcgaaa agaaccagct cgcagaggcg cttcgggagg cccaggtgga gcttcaggct
     7381 ctcgaggcca agaaggtgga gctggagcaa agcagccgca gcgcttcgct cttccaggat
     7441 acgcttcgcg gtgagcgcga agagacgccg agtgacgaca tggggtacgg gcgcatccgc
     7501 gtctccggcg acatggacga cctcggcgag ggttttgccg agatgcgcgg ccagctggcg
     7561 ctaccggtcc agggctcgat gcgaatcggc gaagcgtccc gcgagggagc gccagggctc
     7621 gagttctacg ggcgcgcagg aggcccggtg cgcgcagcag ccgacgggcg agtggccttt
     7681 gcgcggaact acggcgcgta cgggctgatg gtgatcatcg accacggcaa cagtttttac
     7741 accgtatacg ggggccttgg ctccactgac gttcagacgg gcgactgggt cggcatgagc
     7801 actcagatag ggaccttgga catggggtcg cccgaagcga ttctcttctt cgaagttcgc
     7861 cgaggcactc g
//
LOCUS       DASRZF010000060         7520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3482060, whole genome shotgun sequence.
ACCESSION   DASRZF010000060 DASRZF010000000
VERSION     DASRZF010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 7520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7520
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3482060"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..1159)
                     /gene="corA"
                     /locus_tag="VFG22_02750"
     CDS             complement(59..1159)
                     /gene="corA"
                     /locus_tag="VFG22_02750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HET6415189.1"
                     /translation="MFEKHNPEIGSRPGTLAIPPDSPIPKIAVIRYDEHSVERIEITQ
                     PSELQALQEDGRTTWVDVQGLGDEATIRAIGELFNLHPLALENAVNVPQRAKTELYEQ
                     HQLIIARTPRIDDSGRVRAPQVCFIVGKQYLLTFQERYFGFFDPIRERITIGLGAIRS
                     GGPGYLAYALIDAMVDRYYPIVQGLSDSLEELEDLVLENPHPLVLERIRTLRRKLVVL
                     RRVAWPQREALNAMLREQSPFFDAQERVYLRDTYDHIAQIAELIDSSRDTASDMADAY
                     LSNVSHRTNEIMKVLTLMASTFIPLTFVAGIYGMNFEYMPELRSRNGYFVVWGVMVSV
                     AVGMAFYFKRRGWIGTPSAPDFESDRSDDSDH"
     gene            complement(1172..3481)
                     /locus_tag="VFG22_02755"
     CDS             complement(1172..3481)
                     /locus_tag="VFG22_02755"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HET6415190.1"
                     /translation="MSKSNPSESVRPRGRAATISSTAQTPSAWASAGAIALALLLCAS
                     AGAQEFPGFPGAASKEAVQAPEPSTPEAIKSRRVQVASELRLADVALRDANPSQGEHV
                     LARREQLLRIAKLLDEHAAFLSRPAFAPDEIPLPTVDAPSPLALNALYEAWNATTQRA
                     NERDALLEAARDALAAAKQGLDEADRLRRRARAQLAEAEAGETLEAQRTLELRELASR
                     AAQEEVYLRRTEVRALQQAEDGGAGAELERQIEAMRDALESGKGAPAAGSSPLIEREG
                     RLVRRRKAFERRLETVELALEATQGRYLKRADPSAELLAEVEALTAQRDLLREHVALT
                     EGRLARQAQQREIWSYWDALVRGQAPRAELAAWESTTERQLATLADEEREIATRVSEL
                     ERSVGRVEGELFSAPEGSTLHQTLSEQLETVSRVLDKQRTDGTAASADRRVAERVLAN
                     IRDQAELLGWREAAAEVVGTARDVWNYEITTVDDASITIGSVVLAILLFTIGLWAARR
                     GSSLVAHATRRRFELDPGAANALQTLGFYALLVSFTLLALRAVHFPLTAFTVLGGALA
                     IGVGFGSQNVMNNFISGLILMLERPVRAHDVVEVDGNHGTIEKIGARSTQIRSVDGRH
                     IIVPNSFFLESNVVNWTLSDDLIRTKVSVGVIYGSPTRLVEQLMRRVIEEDEQILRSP
                     GPAVVFEEFGDNALNFDIYFWVKARSPMEVRIVQSRVRFHIDELFREHELVIAFPQRD
                     VHLDSATPLEVRVLTHDDAPKSKATRNAT"
     gene            complement(3597..3731)
                     /locus_tag="VFG22_02760"
     CDS             complement(3597..3731)
                     /locus_tag="VFG22_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415191.1"
                     /translation="MQGLQESRILTAHIGDDLVAVGPHKAGRVYKDAAELRRWPAAGV
                     "
     gene            complement(3912..5177)
                     /locus_tag="VFG22_02765"
     CDS             complement(3912..5177)
                     /locus_tag="VFG22_02765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6415192.1"
                     /translation="MSTRAIVPRRILFTYENLLPTQEADAEVIFNTASALAGRGHDVV
                     MSVPAPPDLHPGFEREVLDYYEIEAPLRITPVPSFTKNLALQHVYHAMRLPEHPAFAD
                     ADFVYARNPIVVMRALQGNQRVLMEHYRPWGDQFPPLQPIFRRFMTHPRFLGLVVHSA
                     YAKESYRRLGVPDAKLRVIHNGFDPRRMQPVLSKQEARALLGFPLHGRIVTYTGRMNQ
                     KKGLEVLLELADRMPEVLFVLVGSTGLGPIEIAAESRDNVRVVPWQTSSSISPYLYAS
                     DVLTIPPSSGPLEQVGNTVLPLKLFLYLSAGRAIFAGRTPDTAEILTHDDNAWLVSTG
                     DISEAEHQLRTLLSDANRLERLGQAGLKYAEGLSWEARVAKIEAFFEECLARDPASVD
                     KESWSAAACAQDSLKWLMTGISTGKWIHR"
     gene            complement(5174..>7520)
                     /locus_tag="VFG22_02770"
     CDS             complement(5174..>7520)
                     /locus_tag="VFG22_02770"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET6415193.1"
                     /translation="SRFIALLPDSRPSVQDLEKVSDRVRGLSALTIAVQSPSKNIEAM
                     RRFIRDLVQRLEALPPDEIGIIDWNVGAYDDFVREHKHLYAPLDLLERLRDTLEERHD
                     REVLKNNPFWINFEEDDERESLEDVLDELESESERGEEKSDRFPDGYYQHEDGDLIAM
                     FVRSEIGSDANTAGRIIDSVQAEIDGLDPASYAPDLKVTLSGDVIIAQEEQQAIAREL
                     VLATALTLTAVLLLIVLFFGRPRAVVLLGVGLVPPVLASFAIAQMTVGSLNASTAFLG
                     SIIIGNGVNPNIIWLSRYFEERRAGSDVRNAILGAHRNTWLATMIASGAAGLAYGSLM
                     ITDFRGFRDFGIIGGVGMVLCWVSAITVLPSAVAAYERFRPFPAGRQKTRRWGRFADA
                     FAAVVERVPRGVVAVSILLSLLSAGLVTYAIMQDPFEYDFRNLRSVREEGNSEARLVQ
                     WRVNDILPSHEQGRGIAILVDSLEDVPVIEEQLDAMPPSIHGGHHSLFDFLPQDPNEK
                     LSLLAEIRELSIDIRKRVEDEDTLRKIDDNIPPEVIEIPTLADLPNEVALRFSEKDGT
                     RGRIIVVQESDEFSIWEGRYLVQWAEALRTLRMSDGSRPPLAGRAPVFGDMIGAVYDD
                     MPKAIVAAFLATVVLVLVSFRLRDSLLSIMALLMGILWMAGTMAGLGMKLNFLNFVAF
                     PITFGNGVDYSINVMRRYRLEQQAANPEPILSAVRLSGGAVVLCSLTTIIGYTSLYVS
                     ANQAMNSFGLAMAISEVTCLMAAVLTMPAVMILLSQHARNP"
BASE COUNT         1438 a         2232 c         2348 g         1502 t
ORIGIN      
        1 atcgcccggg gctgcgggga cgcgctcgag ccgaaatcag atcgccggat tggatgtctc
       61 agtggtcgga gtcatcggat cggtctgact cgaagtccgg tgcggatgga gtaccaatcc
      121 acccgcgacg cttgaagtag aaggccatcc ccaccgcgac ggagaccatg acgccccaga
      181 cgacgaagta tccgttacga cttctgagct caggcatgta ttcgaagttc atcccgtaga
      241 tgcccgcaac gaaggttaga gggatgaatg tgctcgccat cagggtcagc accttcatga
      301 tttcgttcgt ccggtggctg acgttcgaga gatacgcatc agccatgtcg gaagccgtgt
      361 ccctcgagga atcgatgagc tcggcgatct gagcgatgtg gtcgtatgta tcgcggagat
      421 atactcgctc ttgggcatcg aaaaacgggc tttgctcgcg gagcatcgcg ttgagcgcct
      481 ctcgctgagg ccacgccact cttcgaagca cgacgagctt gcgccgaagc gttcgaatcc
      541 gttcgaggac gagcggatga gggttctcca gcacgaggtc ttcgagctcc tcgaggctgt
      601 cggagagccc ctgcacgatc ggatagtagc gatccaccat cgcgtcgatg agcgcatatg
      661 cgaggtagcc tgggccccca gagcggatcg cgccgagccc aatcgtgatg cgctcgcgaa
      721 tcggatcgaa gaatccgaaa tagcgttcct ggaacgtgag caggtactgc ttgcccacga
      781 tgaagcagac ctgcggcgcg cgcacgcgcc cgctgtcgtc gatgcgtggg gtgcgtgcga
      841 tgatcagctg gtgctgctcg tacagttcgg tcttggcccg ctgaggaacg tttaccgcgt
      901 tctcgagtgc caaaggatgt aggttgaaga gctcgccgat tgcgcgaatc gtcgcctcat
      961 ccccgagccc ttgcacgtcg acccaagtcg ttcgaccgtc ttcctgaagc gcctgtagct
     1021 cggagggctg ggtgatctcg atccgctcga ccgagtgctc gtcgtaacgg atcactgcga
     1081 tcttgggaat gggggaatcg ggagggatcg ccagagttcc cgggcgagac ccaatttcgg
     1141 ggttgtgttt ctcgaacacc tgttttcctc tctaagtcgc gttccgcgtg gcctttgatt
     1201 tcggcgcatc gtcatgggtc aggaccctca cctccaacgg tgtcgcgctg tcgaggtgaa
     1261 cgtccctctg ggggaacgcg atcacgagct cgtgttctcg gaagagctcg tcgatgtgga
     1321 agcgaacgcg actctggacg attcgcacct ccatcggcga gcgcgccttc acccagaaat
     1381 agatgtcgaa gttgagggcg ttgtccccga attcctcgaa cacgactgcg ggtccaggcg
     1441 atctcaagat ctgctcgtct tcctcgatga cccgccgcat tagttgttcg accagacggg
     1501 tgggcgaccc gtagatcacg ccgacactga ctttggtgcg tatcaagtca tccgaaagcg
     1561 tccagttcac cacgttgctc tccaagaaga aactgttggg gacgatgatg tggcggccat
     1621 ccaccgagcg aatctgcgtg ctgcgagctc cgatcttctc gatcgtcccg tggttcccgt
     1681 cgacctcgac gacgtcgtgc gcgcgaacgg gtcgctcgag catgaggatg agcccgctga
     1741 tgaagttgtt catcacgttc tggctgccga aaccgactcc gatggccagt gcgccaccga
     1801 gcactgtgaa ggcggtgagc gggaagtgaa cagcgcggag tgcgagcagg gtgaacgaca
     1861 cgagcagcgc gtagaagccg agggtctgaa gggcgttggc tgcacctggg tcgagctcga
     1921 agcgccgcct agtggcgtgc gcgaccaaac tggagccccg ccgcgcagcc caaagcccga
     1981 tggtgaaaag cagtattgcg aggaccaccg aaccgatagt gatcgacgcg tcgtcgacgg
     2041 tagtgatttc gtagttccag acgtctcggg ccgtcccgac gacctcggcc gcggcctcgc
     2101 gccagccgag cagctccgcc tgatcgcgga tgttcgccaa gacgcgctcg gcgacacgtc
     2161 gatccgccga tgccgccgtc ccatcggtcc gctgcttgtc gagcacgcgg gacaccgttt
     2221 caagctgttc cgaaagggtc tgatggaggg tcgaaccctc gggagcgcta aagagctcgc
     2281 cttcgacgcg gccgacactc cgctcgagtt ccgagactcg cgttgcgatc tcacgctctt
     2341 catcggcgag tgtggccagc tgtctctcgg tcgtgctttc ccacgcggcc agctcggcgc
     2401 ggggtgcttg tcctcgaacc aacgcgtccc agtagctcca gatttctcgt tgctgcgcct
     2461 ggcgcgcgag tcgtccttcg gtcagtgcga cgtgctcgcg cagtaggtcg cgctgagccg
     2521 ttagcgcttc gacctcggcg agcaactctg ccgatgggtc ggcgcgtttc aagtagcgac
     2581 cttgtgtggc ctcgagggcg agctctaccg tttcgaggcg tcgctcgaaa gctttgcgcc
     2641 ggcgaacgag tcttccttca cgctctatca gtgggctgct gccagccgct ggagctccct
     2701 tgccgctctc gagcgcatcg cgcatcgcct cgatttgacg ttcgagctca gcgcccgctc
     2761 cgccatcttc ggcttgttgc aaggcgcgca cctcggtcct gcgcagatac acttcctctt
     2821 gcgctgcgcg gcttgcgagc tccctaagct cgagggttcg ctgggcttcg agcgtttcgc
     2881 ccgcttcagc ctcggcgagt tgcgcgcggg ctcgccgtcg cagcctgtcg gcctcgtcaa
     2941 ggccttgttt ggctgctgcg agtgcgtcgc gagcggcctc gagcagcgca tcgcgctcgt
     3001 tggctcgctg cgtggtggca ttccaggctt cgtagagtgc gttgagcgcg agcggggagg
     3061 gcgcgtctac cgtcggtagc gggatttcgt ccggagcaaa agcaggcctt gaaaggaatg
     3121 cggcatgctc atcgagcagc ttggcgatcc tgagcagctg ctcccggcgc gcgagcacat
     3181 gctcgccctg cgatggattc gcgtcgcgaa gggctacatc ggcgagacga agctcggaag
     3241 ccacttgaac gcggcgggat ttgatcgctt ctggggtgct cggctcgggc gcctgcactg
     3301 cctctttgct ggcagcgcct ggaaatccag gaaactcttg cgccccggca gacgcgcaca
     3361 ggagcagggc gagcgcaatg gcacctgccg aagcccacgc cgagggggtt tgcgcagtgc
     3421 tcgagattgt cgcggcgcgc cctctcggtc gaacgctttc tgagggattc gacttgctca
     3481 tgacgctttc acgcgaccca ctatagcgtc aaaggcggaa gaacgaaccc gaagagcaaa
     3541 ccgacgttgc tgcgaacgca agatgcattg gcagattgtc ctttggtttc atggcgtcac
     3601 acgcccgccg ccggccatcg gcgaagctcc gcagcgtcct tgtaaacgcg gcctgcctta
     3661 tgtggaccca ctgccacgag gtcatcaccg atatgcgcgg tgagtatccg agattcttgc
     3721 agaccttgca tcgtaacttg gctttccgta ccaaagcctt tcgcggctgg cgcaagaact
     3781 gacctgtccg cgaaatttcg gaccatgtga gttggcccct tcggggtacg caccattggg
     3841 gtccgtgccg ggtcgtcgct tctttagacc ccccactcgt agccctagtg tcccggccag
     3901 gctggaggcg actatcggtg aatccacttg ccggtcgata tgccggtcat gagccacttg
     3961 aggctatctt gcgcgcaggc cgcggcggac catgactcct tgtcgacgct agcagggtct
     4021 cttgccagac attcttcgaa gaaggcctcg atcttcgcca ctcgggcttc ccagctgagc
     4081 ccctctgcgt atttcagacc cgcctgtccg agtcgttcga gtcgatttgc gtcgctgagt
     4141 agggtccgga gctggtgttc ggcctccgaa atgtcgccgg tggaaaccag ccaagcgttg
     4201 tcgtcgtgcg tgagtatttc ggcagtatcg ggagtccttc ctgcgaagat cgcacggccc
     4261 gccgagaggt ataagaagag cttgagaggc aggacggtat tgcctacttg ctcgagtggg
     4321 ccggacgacg gagggatcgt caggacgtcg gacgcataga gatagggcga gatcgaggag
     4381 gacgtctgcc agggaacgac ccgtacgttg tccctcgatt cggccgcgat ttcgatcggc
     4441 cccagccctg tcgacccaac gaggacgaag agcacttccg gcatccgatc ggccagctcg
     4501 agaaggactt cgagaccctt tttctgattc atgcggcccg tgtaggtcac gatccgccca
     4561 tgcagcggaa agccgagcag cgcacgtgct tcttgcttcg agagcaccgg ctgcattcgg
     4621 cgcgggtcga aaccgttgtg aatgacgcga agcttcgcgt caggaacccc aagccttcga
     4681 taagactcct tggcataggc ggagtgaacg accagaccca agaacctcgg gtgagtcatg
     4741 aagcgccgga aaatcggctg aagaggaggg aactgatcgc cccacgggcg gtagtgctcc
     4801 atcaacacac gttggtttcc ctgcaatgcg cgcatgacga caatcgggtt acgcgcatag
     4861 acgaaatctg catccgcgaa cgccgggtgc tccggcaagc gcatcgcgtg atagacgtgc
     4921 tggagcgcga gattcttggt gaagctcggt actggagtga ttcgcaatgg cgcttcaatt
     4981 tcgtagtagt cgagcacttc gcgttcgaac cctgggtgta ggtcaggagg cgctgggacg
     5041 gacatgacga cgtcatgtcc gcggccagcg agcgccgagg ccgtattgaa aatgacctcc
     5101 gcgtctgctt cctgggtcgg caagaggttc tcgtaagtaa acaagatacg tctcggcacg
     5161 atggcgcgtg tactcatgga tttctcgcgt gttggctcag caggatcatg accgccggca
     5221 tcgtcagcac ggcagccatc aaacaggtca cttcgctgat cgccattgcc agaccgaagg
     5281 agttcatcgc ttggttcgca ctgacataga gcgatgtgta tccgatgatt gtcgtcagcg
     5341 agcacaagac tacggctccg cccgagaggc gaacggctga tagaatgggc tcggggttcg
     5401 ccgcctgctg ttcgagacgg tagcgcctca tcacgttgat cgaatagtcg actccgttgc
     5461 cgaacgtgat cggaaaagcc acgaagttca agaagttcag cttcatcccg aggcctgcca
     5521 tggtacctgc catccaaaga atgcccatca gcagcgccat gatagaaagc aggctgtcgc
     5581 gcaggcggaa cgataccagg acgagcacca ccgtcgcgag aaacgccgcg acgatggcct
     5641 tgggcatgtc gtcatagacc gcgccaatca tgtcgccgaa taccggcgct cgaccagcga
     5701 gaggaggtcg cgaaccatcg gacatgcgaa gcgtccgaag cgcctctgcc cactgaacca
     5761 ggtaccgacc ctcccagatc gagaactcgt cggactcttg aacgacgatg attcgaccgc
     5821 gcgtcccatc cttctcggaa aaacggagcg ccacctcatt gggaaggtcg gcgagggtgg
     5881 gtatttcgat gacttctggc gggatgttgt catcgatctt gcgcagcgtg tcttcgtctt
     5941 cgacgcgctt gcggatgtcg atggaaagct cgcgaatttc ggccaagagc gatagctttt
     6001 cattggggtc ttgcggcaaa aaatcgaaca aggagtggtg tcccccgtgg atcgagggag
     6061 gcatcgcatc gagttgttct tcgatgacgg gaacgtcttc gaggctgtcg accaaaattg
     6121 cgatccctct tccctgttcg tgcgagggca gaatgtcgtt tacgcgccat tgcacgagtc
     6181 gtgcttcgga gttgccctct tcacgaacgg accgaaggtt gcggaaatcg tactcgaagg
     6241 gatcttgcat gatcgcgtac gtgacgagcc ctgcagaaag aagagagaga agtatggaga
     6301 ccgctacgac accacgcggc actcgttcga ccacggcggc gaatgcgtcc gcaaagcgcc
     6361 cccaccgtcg agtcttttgc cgtcccgcgg ggaacggacg gaagcgttca taggcggcca
     6421 ccgcggacgg caacacggtt atcgcagata cccagcagag aaccatcccg actccaccga
     6481 tgatcccgaa gtctcgaaag ccacgaaagt cggtgatcat cagagagcca tatgccagcc
     6541 ctgcggcacc ggaagcgatc atggtcgcga gccaagtatt tcggtgggca ccgaggatgg
     6601 catttcgtac gtcggagcct gcgcgccgct cttcgaaata gcgtgacagc cagatgatat
     6661 tgggattcac accgtttccg atgatgatgc ttcccaagaa cgcggtggac gcgttgaggg
     6721 aacctacggt catttgggca atcgcgaagc tggccagcac gggcggcaca agcccgaccc
     6781 ccagcaagac gacggcccgg gggcgaccga aaaacaggac gattagcaga agcacggccg
     6841 taagggtcag cgccgtggcc aagacgagct cgcgcgcgat tgcttgctgt tcttcctggg
     6901 cgatgatgac gtcgcctgac agggtcactt tcagatcggg cgcgtacgaa gcagggtcga
     6961 gcccgtcgat ctcggcctgt acggagtcga tgatgcgtcc ggcggtgttg gcatcgctac
     7021 cgatctccga acgaacgaac atcgcgatga gatctccgtc ttcgtgttgg taatagccgt
     7081 cgggaaacct gtcggacttc tcctctccgc gttcgctctc gctctcgagc tcgtccaaga
     7141 cgtcctcgag gctttcccgt tcgtcatctt cctcgaagtt gatccaaaac gggttgttct
     7201 tgagcacctc tcggtcgtgg cgctcttcga gcgtgtcccg cagacgctcc agtagatcga
     7261 gaggcgcgta caggtgtttg tgctcccgga cgaagtcatc gtaagcgccc acgttccaat
     7321 cgatgatgcc gatttcgtcg gggggtagcg cctcgagtcg ctgtacgagg tcacgaatga
     7381 aacgtcgcat cgcttcgatg ttcttcgagg gagactgcac cgcgatggtg agcgccgaga
     7441 ggccccgaac gcgatcgctg accttctcga ggtcctggac gctgggccgg ctgtccggaa
     7501 ggagcgcaat gaaacggctg
//
LOCUS       DASRZF010000061         2630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4859070, whole genome shotgun sequence.
ACCESSION   DASRZF010000061 DASRZF010000000
VERSION     DASRZF010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4859070"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VFG22_02775"
     CDS             <1..474
                     /locus_tag="VFG22_02775"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET6415194.1"
                     /translation="MKASRHPDIEKALDYLEANKPAEEPIALCWGDSRLANQIFDGVD
                     CVAVLDWEMARLGDPVQDLAWWLASDRCFSEGLELERLPGFPGAEETIAHWTESTGLS
                     AANFDYYTILALTRFSMNMARVGQYMKKIEIIDEENEFDYENLASVTLRRALSEI"
     gene            477..1577
                     /locus_tag="VFG22_02780"
     CDS             477..1577
                     /locus_tag="VFG22_02780"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HET6415195.1"
                     /translation="MGPLHGIRIVEIEGIGPCPFAGMLLSDLGAEVIRVGRPEPSFLQ
                     ASFDVMSRGKKSITLDLKKPEEKAALLKVVATADALIEGFRPGVMERLGIGPEVCMEV
                     NPRLVFGRMTGWGQEGPWSKRAGHDINYIALAGALHAIGRKGERPTIPLNLVGDFGGG
                     GLLLAFGVVTALLEAKTSGKGQVVDAAMVDGVAALMASIYAGMQQGFWSDKRGENLLD
                     SGAAFYDVYETRDGKFVSIGALEPQFYKELVEKLGIEQSAAAAGAHLNPAKWGELRPT
                     LEELFRQKTRDEWCEVFEGSDACFAPVLAAAEVFEHEHHVARRSFVEVDGIMHAVAAP
                     RFSRSKPADPTPAKERGADTEAILRECGVELP"
     gene            1590..>2630
                     /locus_tag="VFG22_02785"
     CDS             1590..>2630
                     /locus_tag="VFG22_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415196.1"
                     /translation="MITDFDDYLIHQAPFPINQTGIVNRNAYDRYWFNGFDKAGTFLF
                     EAGFGRYPNRFVQDAHFSVAVDGVQHAFHASRRIADNPMDSSAGPLRVQVLEPMRRIR
                     LLIEPNETGVECDLTFHAKAPPVQEPKNVMYDGNRLIMDTQRFTQLGTWEGHFSIKGE
                     RFEVKRGEVIGLRDKSWGMRPVGEYEEGAPSRLTTDPAVYWIWSPIHFDGFCTHFLTR
                     EMPDGHAQELSASIVRTYGDDEEVPKDVLEADEEVMASATNSIKWQSGTRWPEAATLG
                     FVKRDGTKLEIELDPIFRFQMMGIGYQHPEWGHAVWRDELEIGYESWKLDEVEPDDYC
                     SIHVHNLVRAKMG"
BASE COUNT          550 a          772 c          841 g          467 t
ORIGIN      
        1 atgaaagcct cgcggcatcc ggacatcgaa aaggcgctgg actacctcga agcgaacaag
       61 cccgcggagg agcccattgc tctgtgctgg ggtgattcgc ggctcgccaa ccagatcttc
      121 gacggcgtcg actgcgtggc cgtgctcgac tgggaaatgg ctcgcctcgg cgacccggtg
      181 caggacttgg catggtggct cgcgtccgat cgatgcttct ccgaaggcct cgagctcgag
      241 cgtctgccgg gatttcccgg cgcggaagag accattgcgc actggaccga atcgacggga
      301 ctgagcgcgg ccaacttcga ctactacacg attctcgcgc tcacgcgctt ttcgatgaac
      361 atggcgaggg ttggtcagta catgaagaag atcgaaatca tcgacgaaga gaacgagttc
      421 gattacgaga acctggcgtc agttacacta cgccgcgcgc tatcggagat ctgaagatgg
      481 gtccactcca cggaattaga atcgttgaaa tcgaaggcat tggtccctgc ccgttcgcgg
      541 ggatgctgct ctcggacttg ggagctgagg tcatccgggt gggtcggccg gagccatcct
      601 ttcttcaagc gagcttcgac gtcatgagtc gcggcaagaa gagcatcacg ctggacctca
      661 agaagcccga agaaaaggcc gcgctgctga aggtcgtcgc aaccgccgac gcgctgatcg
      721 agggatttcg ccccggtgtg atggaacgcc tggggatcgg acccgaggtc tgcatggagg
      781 tcaaccctcg cctggtcttc ggccgcatga ccggctgggg acaggaaggc ccctggtcaa
      841 aacgggccgg acacgacatc aactacatcg ccttggcagg ggcgcttcac gccatcggcc
      901 ggaagggcga gcgaccaacc atcccgctca acctggtggg cgactttggc ggcgggggct
      961 tgttgctcgc attcggtgtg gtgacggcgc tgctcgaagc gaaaacctcg ggcaaggggc
     1021 aagtggttga cgcggccatg gtcgacgggg tcgccgcgct gatggcaagc atctatgcgg
     1081 gcatgcagca agggttctgg tccgacaagc gaggggaaaa cctgctcgat tcgggggctg
     1141 ctttctacga cgtctacgag acccgcgacg ggaagttcgt ctcgatcggc gcgctcgagc
     1201 cgcagttcta caaggagctc gtcgaaaagc tcggcatcga gcagagcgcg gcagctgccg
     1261 gcgcgcacct gaaccctgcg aaatggggcg agcttcgacc gaccttggag gagctctttc
     1321 gtcagaagac gcgagatgaa tggtgcgagg tcttcgaagg tagcgatgcc tgcttcgcac
     1381 ccgtgctggc tgctgccgag gtattcgaac acgagcacca cgtcgcacgc cgttcgttcg
     1441 tcgaggtgga tggcatcatg cacgccgtgg cagctccacg attttcacgc agcaagccgg
     1501 cggacccgac gcccgccaag gagcgcggcg ccgacaccga agcgattctg cgcgaatgtg
     1561 gggtcgagct accttaggaa gaacgcgaga tgatcaccga cttcgacgac tatctgatcc
     1621 accaggcacc gtttcccatc aatcagacgg ggatcgtcaa ccgcaacgcc tatgaccggt
     1681 actggttcaa tggcttcgac aaagccggga cgtttctctt cgaggccgga ttcggacgtt
     1741 acccgaatcg cttcgtccaa gacgcccact tcagtgtcgc ggtcgacggt gttcagcatg
     1801 cgttccatgc atcgcgtcgc atcgccgaca acccaatgga ctcgagcgct ggtccgctga
     1861 gagttcaggt actcgaaccg atgcgccgga tccgcctcct gatcgagcca aacgaaacgg
     1921 gagtcgagtg cgacctcacg tttcacgcaa aggcgcctcc agttcaagaa ccgaagaacg
     1981 tgatgtacga cggcaaccgc ctgatcatgg acacgcagcg cttcacgcag ctcggcactt
     2041 gggagggaca tttctcgatc aagggcgagc gattcgaggt caaacgcggc gaggtcatcg
     2101 ggcttcgcga caagtcctgg ggcatgcgcc ctgtcggtga atacgaagag ggcgcgccga
     2161 gtaggctcac cacggatccg gcggtctatt ggatctggtc gccgattcac ttcgacggct
     2221 tctgcacgca cttcctgacc cgcgagatgc ccgacgggca cgcgcaggag ctctccgcgt
     2281 cgatcgtgag aacctacgga gacgatgaag aggttcccaa agacgttctc gaagcggacg
     2341 aggaggtcat ggcctcagcg accaactcga tcaagtggca aagcgggacg cgctggcccg
     2401 aagcggccac gctcggattc gtgaaacgcg atgggaccaa gctcgagatc gagctcgatc
     2461 cgatcttccg gtttcaaatg atggggattg gataccagca ccctgaatgg ggccacgcgg
     2521 tctggcgcga cgagctcgag atcggatacg agagctggaa gctcgacgaa gtggagccgg
     2581 atgactactg ctccattcac gtacacaatc tggtccgcgc caaaatgggt
//
LOCUS       DASRZF010000062         4723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4171113, whole genome shotgun sequence.
ACCESSION   DASRZF010000062 DASRZF010000000
VERSION     DASRZF010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4171113"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            575..2455
                     /locus_tag="VFG22_02790"
     CDS             575..2455
                     /locus_tag="VFG22_02790"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HET6415197.1"
                     /translation="MTSAVSTNDHRWALRSNFMKALLAGVPPLLVYLSTASGYAHWLD
                     SGEFVAVAADFGISHPPGHPLAAVVLGAANLLPFGALSFRVALICAALLGLANVALFF
                     ATEETLKAGGVIRQSLRFPLALAATWWVAGTEAWWFQAVRPEVYALQAVLLTIAIERL
                     LRVSLSEDGDDVRPLYHAALALGLALANHHYLAILIVLPSLWLLLGVWQRWGWRPFAW
                     SSAFVGAGLLTYIYLPLRALREPFLNLGDPSSLGRFFWVVSAQAYQKSISPEAVAPFS
                     ERMADVLMVTGEDMHIATLIVAVLGAYFMLRVKSARRYGLFWLTLWLVYVLGRAVLGF
                     VRGNPDAVAYFMLSYASLAIFAVFALGVLLSALAEAVPSRPRLTPAIGLLLALGASSH
                     FFLSSQASSLARFVDTDVFDDGLRRALPPRAVVLAHNPQTIFRYWGGEAEELNRPDVT
                     MIPLPLLTYPKLVERFVEDEPELKPLLRSYMLDGALSTPELQSLAALRPVFVEMDVRV
                     SQEMMELLVPEQLYHRVLTSETTPTDEAHATENHTALWADLYQRITRPLEEHTKTQLL
                     WWHYADSLYFASVGDTSAAMRTVTAGLALNPHAEELVRLRAALDKAEPGDRLDITPFT
                     VR"
     gene            complement(2465..2959)
                     /locus_tag="VFG22_02795"
     CDS             complement(2465..2959)
                     /locus_tag="VFG22_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415198.1"
                     /translation="MTGVRLLISIALAALLIAACRSEPAPTGFESPSATIDSLFRAYG
                     VQDISQDEARRRLASRERFELLDARLFKSCFSDWQGEHDHALGGFVFGQLVAAKDELQ
                     ISAEPDSAEVRAASASAELQPIVLVNQDGAWKIDLRESVTPEVRQSLYEVYRRARRAE
                     RQAR"
     gene            3026..>4723
                     /locus_tag="VFG22_02800"
     CDS             3026..>4723
                     /locus_tag="VFG22_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HET6415199.1"
                     /translation="MRLDRLTTKTREALVAAQQNATDRGNPEVYPEHLMLDLLAQAGG
                     LAPAILEKAGADLQGLIRAFGAVLDRMPKVAGGAEPGISSRLRDVLNNAWKETEAFKD
                     EYTSAEHILLAAYNDKQLKTALEAQSVDRTRLLQAVEQVRGSQRVTDPDPEGKFQALE
                     KYTRDLTEAARQGKTDPVIGRDEEIRRVMQVLSRRTKNNPVLIGEPGVGKTAIAEGIA
                     HRIVQGDVPEGLKNKKFLALDLGALVAGSKYRGEFEERLKAVLQEMESAKGEIILFID
                     ELHTLVGAGAAEGSMDASNMLKPALARGELRCVGATTLDEYRKHIEKDAALARRFQPV
                     FAGEPSVTDTIGILRGLKERYEIHHGIRIQDSAIVAAATLSERYITDRFLPDKAIDLV
                     DEAASRLKMEIDSLPAPIDTAERKLTQLEIEREALKKEKDDASKRRLGDLEQELSELR
                     EQRDTMRAQWLREKDLITEIREKKELVETLRLDLERAERTTDYEKAARLQYGEIPAAE
                     LAVTKAQQKLAEVQTSASFLKEEVTEEDIAEVVSKWTGIPVNKMLEGEKDKLLHMEDR
                     "
BASE COUNT          876 a         1495 c         1464 g          888 t
ORIGIN      
        1 ttccgcacgc cttcccaggg ctcttggcga tcacgggcga ggtcttcgat cgagtatgtg
       61 ctgggctcac tcttcatgag ccagagcttc ttcgcgcgtt tcggcatcgc gcgagtatgc
      121 cacggatacg ccggccccag cacggtggag aaaagtacga ccggagaacg gctcgcgacg
      181 cacgtgctcg caagtgggac ccgctcggcc gtcccttcaa gactccatac gtctagcctc
      241 ccgcccactt gctgtgccgc gcatcccgag ccgttctcct atgttgagct tctcatgccc
      301 gcactcacgc gcacactgac atctgcatac ggaggggggc gggggcacgc gtcatggggt
      361 ggagtcgacg tcgcaagtgc caatcggtca gggagcaacc acctccgatt ggcgcgcgcg
      421 ccgaggctcg gcttgcgtgg tccaagctct caagtgaatc cgaccacaat tgcaagccga
      481 gccgttcgac ggaaccccgt gtcgtgtgcc cccgcccccc ggtcgaaatg cgagcacgtg
      541 cgtcgcgagc cgttctccgg tttcgttcga tgatttgacc tcggcggtat cgacgaacga
      601 tcatcgctgg gccttgcgct cgaacttcat gaaggccctg ctggccggcg ttcctcctct
      661 gctcgtctac ctgagcaccg cgagcggata cgcgcactgg ctcgacagcg gggagttcgt
      721 tgcggtcgct gccgacttcg gcatctcgca cccgccgggt caccctctgg ccgcggtcgt
      781 cctcggtgct gcgaatctcc tgccgtttgg agcgctttcg ttccgggtcg cattgatctg
      841 cgcggctctt ctcgggctcg cgaatgtggc cctcttcttc gccaccgagg agaccctgaa
      901 agccggcgga gtgatccgtc aatcactgcg gtttccgctt gccctcgccg caacctggtg
      961 ggtagcagga accgaggcgt ggtggtttca ggcggttcgc cccgaagtct acgcactgca
     1021 ggcggtcttg ctgaccatcg cgatcgagcg attgcttcgc gtttcgctct cggaagacgg
     1081 cgacgatgtg cgtcctctct accacgcagc attggctctt ggcctggcgc tggccaatca
     1141 tcactatctc gcaatcctga tcgtgcttcc gtccctctgg ctcctgctcg gggtctggca
     1201 gcgctggggc tggcgaccgt tcgcctggtc gtccgcattc gtgggcgccg gactgctcac
     1261 ctatatctac ttgccgctgc gcgcactgag agagccgttt ctcaatttgg gggacccttc
     1321 gagcctcgga cggttcttct gggtcgtgag cgcgcaggct tatcagaaga gtatttcgcc
     1381 agaagcggtc gctccttttt cagagcgcat ggccgacgtc ctcatggtga ccggcgagga
     1441 catgcacatc gcgacactca tcgtcgcagt tctgggcgcg tacttcatgc tccgagtcaa
     1501 gtccgcccgc cggtacgggc tcttctggct cacactctgg ctcgtgtatg tgctggggcg
     1561 cgcggtgctt ggtttcgtca gaggcaatcc cgacgccgtc gcctacttca tgctctcata
     1621 tgcgtcactc gcgatcttcg cggtgttcgc gctgggggtc ctgctctccg cgctggctga
     1681 agcggtgccg agccgaccgc ggctcacgcc ggcgatcggg ctgcttttgg cgcttggggc
     1741 ttcgagtcac ttctttctct cgtcccaagc gtcgtcgctt gcgagattcg tcgataccga
     1801 tgtgttcgac gacggtctcc gccgcgctct gccgccacgc gcggtcgttc tcgcgcacaa
     1861 cccgcagacg atctttcgat attggggagg cgaggctgag gagctcaacc gcccggacgt
     1921 gaccatgatc ccgctcccct tgctgaccta cccgaagttg gtcgaacgat tcgtcgagga
     1981 tgaaccggag ctcaagcccc tgctccgaag ctacatgctc gatggcgcgc tgagcacacc
     2041 ggagctccag agtctcgcgg cgcttcgtcc ggttttcgtc gaaatggatg tccgggtcag
     2101 ccaggagatg atggaattgc tggtcccgga gcaactctat caccgagtgc tcacgtccga
     2161 gacgaccccg accgacgaag cgcacgcaac ggagaaccac accgcgctct gggcggatct
     2221 ctaccagcgc atcactcgtc ccctggagga gcacacgaag acccagctcc tctggtggca
     2281 ctatgcggat tctctctatt ttgccagtgt cggcgacacg agcgcggcca tgcggaccgt
     2341 caccgcaggc cttgcgttga accctcacgc cgaggagctc gtgaggctgc gtgccgccct
     2401 cgacaaagcg gagccaggcg atcgtctcga catcacgcca ttcacggtcc gctgacgact
     2461 accgctagcg ggcttggcgt tcggctctgc gagcgcgtcg gtacacttcg tagaggctct
     2521 gccgaacctc aggcgtgacg ctctcgcgca gatcgatctt ccaggcgccg tcttggttga
     2581 cgagcacgat cggctgtagc tctgcggagg ctgacgccgc tcgcacctct gcggaatccg
     2641 gctcggcact gatctggagc tcgtctttcg ccgcgacgag ctgaccgaac acgaagccgc
     2701 cgagcgcgtg atcatgctca ccctgccaat ccgaaaaaca gctcttgaaa agccgagcat
     2761 cgaggagctc aaatctctcg cgcgacgcca agcgccgccg cgcttcgtct tgagagatgt
     2821 cttgcacccc gtaggcgcgg aagagagagt cgatggtcgc gctcggcgat tcgaatccgg
     2881 tcggtgctgg ctcgctcctg caggcggcga tcagcaaagc ggctagggcg atggaaatca
     2941 gcaaacgcac cccagtcata tggccctcat ttgtgccact gtctaactat tgacgcaccg
     3001 tttgacgtgc ttaatgtggg tccaaatgag actcgacaga ctcaccacga agacgcgaga
     3061 ggccctcgtt gcggcccagc aaaacgcaac cgatcggggc aacccggagg tgtatcctga
     3121 gcacctcatg ctcgatttgc tggcacaagc cggggggctt gcgcccgcga ttctcgagaa
     3181 ggcgggggcc gacctgcaag ggctgattcg cgctttcggg gccgtgctcg accggatgcc
     3241 caaggtggca ggcggcgcag agccgggcat ttcctcgcgc ctgcgtgacg tgctcaacaa
     3301 cgcctggaaa gaaaccgagg ccttcaaaga cgagtacacc tcggccgagc acatcttgct
     3361 cgctgcgtac aacgacaagc agctgaagac ggcactcgaa gcgcagagcg tcgaccgcac
     3421 cagactcttg caagccgtgg agcaggttcg cggcagtcag cgtgtgaccg acccggatcc
     3481 ggaagggaag ttccaagcgc tcgagaagta cacgcgcgac ctcacagagg ccgcgcggca
     3541 aggcaagacc gacccggtga tcgggcggga cgaggaaatt cggcgagtca tgcaagtgct
     3601 ctcgcgacgg accaagaaca atcccgtcct gatcggtgag cccggtgtcg gtaagaccgc
     3661 gatcgcggaa ggtatcgctc atcgaatcgt ccagggggac gtgcccgagg ggctgaagaa
     3721 caagaagttc ctcgcgctcg acctcggcgc attggtcgcc gggtctaaat accgcggtga
     3781 gttcgaagag cgcctcaagg ccgtgcttca agagatggag tcggccaagg gcgagatcat
     3841 cctcttcatc gatgagctgc acacgctcgt cggggcaggg gccgcagaag gctcgatgga
     3901 tgcgagcaac atgctgaagc ctgccttggc tcgaggtgag ctccgctgcg tgggggcaac
     3961 gacgctcgac gagtaccgaa agcacatcga aaaggacgcc gcgctggcac gccgcttcca
     4021 gccggtcttt gctggagaac cttccgtgac cgacaccatc ggcatcttgc gaggcctcaa
     4081 ggaacgttat gagatccacc acgggatccg aattcaagat tcggcgatcg tcgctgcagc
     4141 cactcttagc gagcgttaca tcaccgaccg gttcttgccc gacaaggcga tcgacctggt
     4201 ggatgaagcc gcttcgcgct tgaagatgga gatcgactcg ttgccggcgc cgatcgacac
     4261 ggccgagcgc aagctcactc agctcgagat cgagcgcgaa gcgctcaaga aggaaaagga
     4321 cgacgcgagc aagcggcgcc tcggtgatct cgagcaggag ctctcggagc ttcgtgagca
     4381 gcgcgacacg atgcgggcgc agtggctgcg cgaaaaagac ctcatcaccg agatccgaga
     4441 aaagaaagag ctcgtcgaga cccttcgtct ggacctcgag cgtgccgagc ggacaacgga
     4501 ctatgaaaag gcagcgaggc tgcaatatgg cgagatcccc gctgcggaac tggccgtgac
     4561 caaggctcag cagaagctcg ccgaggtgca gaccagtgct tccttcctga aggaagaggt
     4621 caccgaggaa gacatcgccg aggtggtttc caagtggacc ggcattccgg tcaacaagat
     4681 gctggaaggc gaaaaggaca agctccttca catggaagac cgg
//
LOCUS       DASRZF010000063        19175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152 k141_44569,
            whole genome shotgun sequence.
ACCESSION   DASRZF010000063 DASRZF010000000
VERSION     DASRZF010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 19175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19175
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44569"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1298)
                     /locus_tag="VFG22_02805"
     CDS             complement(<1..1298)
                     /locus_tag="VFG22_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415200.1"
                     /translation="MEYYSDGNILSVLAFFIWVPVTYFATWRWPPSKATAVLFLGGLL
                     LLPELVFFKPPGLPDFAKLEIVPMWILIWAAVFHRERFASAPKSRWFRVCVGLLVFGS
                     VFTVFLNTDPYSVGHRAVPGHVPYDAVHAALNALLSVALPFYLGAVMFRGSRDLRVLL
                     TTLVVAALLYSPLQFIEMILSPQLHRWVYGFHQHAFLQVMRGDSYRPMVFMAHGLAVA
                     IFTTLALIAAAALFKTRLRILRFRAAWVMSYLWVVLILSRSLASLLYSMAAVPLVLFT
                     SPRVQALVAAALAGLLFVYPLLRANDLIPVEVLQAWAEDDYGQERAQSMTFRFENEAR
                     LLDRALERPWFGWGSYCRACVYERWSGERSPVSIRDGAWIIRFGDQGIVGFVSSFGLL
                     LFPLIALVRRLKYAPRASDRRLLAGLGLMLGFSAFDLIPNG"
     gene            1646..2677
                     /locus_tag="VFG22_02810"
     CDS             1646..2677
                     /locus_tag="VFG22_02810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6415201.1"
                     /translation="MVPAHDEEEGIGAMLAEVQSQLAPDDSVLVVADNCTDETVRLAR
                     AAGAEVIERTDAERRGKGYALAYGIEHLTPSPPDVLIVIDADCRLTPGTINALVRSAV
                     ETMGPVQADYVLQAIDRSPISMISALAFLVRNRVRPRGLLRLNQPCHLDGSGMAFPWS
                     VVRTAPGLGANIVEDLTMGIELALLGHEPQLCIDAGVRSDLPSSRPASMRQRRRWEHG
                     QMAALLEYGPRLIREGVLRGRLGLIALGADLMVPPLALLVGLLVLVLGAAGVLVLVGG
                     STVPAWIAVVALGLIGLGVAVGWARYGRKEIPLRYLLLVPIYVVWKLPLYLSFLVGRR
                     ERRWRRTDR"
     gene            2900..4060
                     /locus_tag="VFG22_02815"
     CDS             2900..4060
                     /locus_tag="VFG22_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415202.1"
                     /translation="MLSERDTATTAGRGFFNAGTPFMGRVLRDAVGRASPPQDREAHL
                     GAAVDWLKRAQAATPDGGVSYGYSIRGGWRPSYRETSGYIAVTLFDLAKQRQDSESAA
                     LARTVSNWLCEVQEPDGSIANPQYRPHQGIVFDTGQVLLGYVRAHRETGESRFRDAAV
                     RAASWLADHIDEDGAWRRNTHLETPHTYNTRVAWSMLECHQIASDPAIEAAARKNLDW
                     ALGQERDGYFRNCAFTPRAAPFTHTIAYAIRGFLESARLLGDACYEEVAVRAADAVLE
                     HVREDGWIPGQIDEDGRSAARYCCLTGNCQLAIIWFKLYQSTGSERYLDAAKRSLGFV
                     MEHQDLSTSNPALRGAIKGSHPIWGSYAPLSYPNWAAKFFIDAVLLWDEIEP"
     gene            4057..4812
                     /locus_tag="VFG22_02820"
     CDS             4057..4812
                     /locus_tag="VFG22_02820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HET6415203.1"
                     /translation="MKSVRILNGDLHAATLEGTTDWAREWIRSGRRGYICTVNVAILM
                     MMRSDRRLQGFVDRASLVVADGQALVWASHLQGNPLPERVTGVDLVDELCALAAKESF
                     GVYFLGAKPTIIKTAAKRLAERHRGLEICGISDGYFDASEAPRRAEAIRKSGARILIV
                     GMGVPLQEEFIENHWDQLGVELAIPVGGSFDVIAGAVRRAPVWLQRIGMEWFFRLLQE
                     PRRLWKRYLVTNTQFIFHLLRSVAGSDPGRSSN"
     gene            4818..5933
                     /gene="wecB"
                     /locus_tag="VFG22_02825"
     CDS             4818..5933
                     /gene="wecB"
                     /locus_tag="VFG22_02825"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HET6415204.1"
                     /translation="MPTKTRILIVMGTRPEVIKLAPVVIGARSRPEEFDTFVVTTGQH
                     REMLNQMLAVFQIAVDLDLDIMKPNQDLRYITTAALTGLHDVVGQVKPDWVVVQGDTT
                     TTLAGALAAFYQHVPVAHVEAGLRTYDKRQPFPEEMNRRLTTQIADAHFAPTTLSRDR
                     LLREGVSEEAIWVTGNTGIDALFLILGSNAGETAPQPTGERRLLVTAHRRENQGEPMR
                     QMCRALLRLVDEFSDLRVVYPVHLSPAVRDVIFPLLGNHSRVELVEPLEYRRFVEEMR
                     RAHIILTDSGGIQEEAPSLGKPVLVMRETTERPEGVDAGTARLIGTDEERIYSETARL
                     LSDDDAYTEMAQARNPYGDGRASERILTALAKGYRQR"
     gene            6067..7230
                     /locus_tag="VFG22_02830"
     CDS             6067..7230
                     /locus_tag="VFG22_02830"
                     /inference="COORDINATES: protein motif:HMM:NF044715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415205.1"
                     /translation="MTIPSDSSPIRVGVAGTGFIARRLFDSCRLMDDLQVSSALTRRA
                     LDSIKDMPSDVQLTQSTQELIDGSDLVVECSGDPIRAAEVVRDTLAAGLPVVTMDSEF
                     HVTCGSHFVGKGYLSEANGDQPGVQAQLHDELVGMGFEPLVYGSMKGFLNPNPSLKDM
                     AYWSERQGFRVQQTTSFTDGTKVQIEQAFVANAFGAGIAKPGLLAVQNDSVEEAARAL
                     AEAALELGHPIADFTVVPGQAPGVFVIATIAESQRPVLAALKMGDGPYYTFVRPYHLC
                     ALEIPNTIRRAMEGQPPLLNNSTAPSIGVAAVAKRSLRPGEHIETGIGSFQVRGTAVR
                     FRDEPDHLPIGLLYGATVTRPVEPDQMLTLDDVDLPDSYARDITLGLIRDACG"
     gene            7324..7809
                     /locus_tag="VFG22_02835"
     CDS             7324..7809
                     /locus_tag="VFG22_02835"
                     /inference="COORDINATES: protein motif:HMM:NF021082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2383 domain-containing protein"
                     /protein_id="HET6415206.1"
                     /translation="MRSSTSIHAAHLRPMTHFDARQADIDLLNAFLCRERSAREAYGV
                     LLEASSDEQIRRALARLRNSHQERINELEGRIRALGGIPRAQSSLWRTLLRAREQSAG
                     RLNKGTVIFTLEDYSSRILRAYRRSLTDLSPESRKFIERKILPESQIVHQSVISLQKA
                     L"
     gene            complement(7806..7931)
                     /locus_tag="VFG22_02840"
     CDS             complement(7806..7931)
                     /locus_tag="VFG22_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415207.1"
                     /translation="MHASPLERMSIGTRLFSPETQSVEKGRLERERACKGLAAAG"
     gene            7985..8887
                     /locus_tag="VFG22_02845"
     CDS             7985..8887
                     /locus_tag="VFG22_02845"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET6415208.1"
                     /translation="MSPLGLGLSVLIGISLGFFGGGGSILTVPLLVYVFGLDPKTAIA
                     SSLLVVGAASTSGALHHWRAGNVDVRTGLLFGAAGMTGAYLGGRVGAFLDGTLLLLLF
                     ACMMVLTSVGMWQGRRARSPGGATKRATGRLLLQGLAVGSFTGLVGAGGGFLIVPALT
                     LWAGLSMHAAVGTSLFIIVLNTMAGFLGYLSHVQVDYTLIGAVTVMAIGGSFIGSRLA
                     SQVNPASLRRAFAAFVLTMAIFILVREADQWITTARDALPGTTPQIVFALLMLGIGIA
                     AGRVTRRAAREPTADRLFSDGGGI"
     gene            complement(8867..9478)
                     /locus_tag="VFG22_02850"
     CDS             complement(8867..9478)
                     /locus_tag="VFG22_02850"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6415209.1"
                     /translation="MHVAKPRSGAQRVLSHEEAKAFDDQFGPKQDLQRIYEDPAIEVL
                     LEYASFQTACAVVELGCGTGRLAARLLEELLPEDSKYLGLDISETMIDLAHGRVAPWG
                     DRARMQRTDGSPTLPVRRFHRGSFAALGAQCTRSAQGSLVDAGLSTSRNSSAVTGRSF
                     TAKSSARSVSAPRYWSRGSSPVEAIVMDPMTRVAVRSSDSAAV"
     gene            complement(9545..10606)
                     /locus_tag="VFG22_02855"
     CDS             complement(9545..10606)
                     /locus_tag="VFG22_02855"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6415210.1"
                     /translation="MLTGRYSPSRALLFLALALVSPAAASPVEDALGPAPRGGDGRFM
                     NFAGHMPEVGVSVTLPFFVRRIASSFTDTPSALDRVPNDGAWLRENAKHSEPTVTWIG
                     HATLLVQMGHLTFLTDPIWSDRASPLSFAGPQRIVEPGVAIDDLPPIDFVVVSHNHYD
                     HLDLASLIALSERDPNTRFFVPLENGKLLRDNGVENVEELDWGQSVELGGVRVYCLPA
                     QHWSKRGIGDDREALWSSWAVLAPERRFFFAGDTGYFDGFARIEEALGPFDLAAVPIG
                     AYEPAEMMKASHMNPEEAVQAAIDLRARAAVAMHYGTFKLSDEPLDEPAKHFKKAATE
                     SALGDEAGWLLRIGETRAF"
     gene            10722..11333
                     /locus_tag="VFG22_02860"
     CDS             10722..11333
                     /locus_tag="VFG22_02860"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HET6415211.1"
                     /translation="MMHPSTELRHIDPELGFGVFATSRLPKGTITYVWDPLEIQIMPD
                     DPSLQEPALRESIDRFSYIDSHGVRIVSWDHAKYVNHCCQSNTMSTAYGFEIAVRDIE
                     AGEQVTDEYGMFNLRDPMKLRCSSGPCRGEVRAADLERFCLVWDEQIKAALELVEQVH
                     QPLWPLIDPLLATEVERYARWGEGYRSVRELGEIKASDRAHSG"
     gene            complement(11335..12252)
                     /locus_tag="VFG22_02865"
     CDS             complement(11335..12252)
                     /locus_tag="VFG22_02865"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HET6415212.1"
                     /translation="MEKAIQNAMKLLDDPAISKLAGTLIAVVIVVAIVQVIKRVATRY
                     IEDKSVRYRARKAIGFIGYLLGALVVLAMFSDRLGRMSVVFGVAGAGIAFALQEVIVS
                     VAGWAAVSFGSFYTVGDRIQLGGIKGDVIDIGVLRTTLAEVGQWVDGDLYNGRMVRVA
                     NSFVFKEPVVNYSGDFPFLWDEITVPIRFGSDLDMAQAALTEAANEVCGAYAEQATTI
                     WARMQRRFPLEDASTVPMVTFGFDENWVKFTIRYTVQFDRRRATKHLLSARVLKAIES
                     SGGAISVAAAAIELFPKAPLDIRQLATKD"
     gene            complement(12290..14332)
                     /gene="fusA"
                     /locus_tag="VFG22_02870"
     CDS             complement(12290..14332)
                     /gene="fusA"
                     /locus_tag="VFG22_02870"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET6415213.1"
                     /translation="MPDFGTPDFHNVLLAGSRSSGKTTLAEALLFRAGETSRQGSVDG
                     GTTTSDFELEEQAHQYSVYSTLMHATHQGKRINLLDLPGAPDFIGQAIACLPAAETMM
                     LVMDPEVGVDSASRRLVKLARSLDLPIAIVVSHIGQGVSRLGAFLSELQEAFGRGCLP
                     INLPANKGEAVIGCLLNGTGESDLGPVAGFHTELLDQVVEIDDALMERYLEGEEPNYE
                     ALHEPFERAMDKAHVIPVCFTDALSGAGVGELLDIIVRYFPSPPEGNPRPFFIGEGAD
                     EKPFVYSDDADKPLLARVFHVATDPYLGKLCFFRVYQGKLNAGDQLFVGDGKRPIRIG
                     HLFRTQGKQRTAIHSVIAGDVGVIAKLDELHLGDVLHDDHALDLVHHKPLPYPKPLHG
                     LAVVPKTRSDEDKIAEVLARIREEDPTFEAHFELETRELVIHGLGEMHLNLVIERIKN
                     QGVEVETKPPKIAYHETVMGEAEGQYRHKKQSGGAGQFGEVSLRVEPLERGQGFEFVD
                     KIVGGVIPKVFLPAIEKGVRDMMRRGIVAGYPIEDVRVTVFDGKTHAVDSKEIAFRTA
                     GKFAFKEAFLKARPQILEPVVHVEVTTPGDHVGTITGDLSSRRGKIFVTDVKSTGTAI
                     VKATVPLADMMEYEPALKSMTKGRGSFTMELSHYDPVPDRIAQELIEKHQAERLST"
     gene            14429..14971
                     /locus_tag="VFG22_02875"
     CDS             14429..14971
                     /locus_tag="VFG22_02875"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET6415214.1"
                     /translation="MAELNRLDPVTLDFFDTAPLRVQCTMLAKCTPERLFETLRGDTV
                     WTEWAGVIKGVDWTSEKPYGKNATRDVSLVGGMVVKELFFHWEENARIAFYVTESTIP
                     GLSKFAEDYRVERVGPNETKLSWTVAIENQGLMRHLNSVTAPVMKLVFQGWLKKYKKI
                     LETPVAKPAAQPIAAPQPRA"
     gene            15111..18668
                     /locus_tag="VFG22_02880"
     CDS             15111..18668
                     /locus_tag="VFG22_02880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CxxxxCH/CxxCH domain-containing protein"
                     /protein_id="HET6415215.1"
                     /translation="MSRRSALDPLRSNSGGDRDGDGAGTAAANISQAPSRVQMLQAAG
                     LEERTLRTVLGERAKNQILWYSTCLPFIDVIMRRFSRSCRGLSIAVLCASVLIGCGTD
                     GGEIGQPDGGLPDGGEGGVGGSGGSAGVGGIAGSGGSAGTGVSGRFHRSDYEKPELHG
                     SDLRLGVLDCRLCHGKDLSGGGDIEPSCDTCHTPAEPEAWRAECVFCHGGVEDLSGAP
                     PKNLDGTLTVAASAFVPHHAHVRTAMSEPLDCIECHVNVTDVLTQGHVFDDTPAVAEV
                     DLGGGRSPRGMYDPVDGCASLYCHGNGQGDNGSLGAADPPMTCESCHAGASSPSVELA
                     KMSGRHGFHISIGAGCQDCHQATTSDGLSITSPSLHINGQRDNQFSAPGFSYDQGTQG
                     CTGTCHGYPHSARPWIGSSQGFHPVGFADPEMHSPAMELQRMDCRTCHGADLSGAAGP
                     SCDSCHTVGWRRNCLFCHGAGDTLTGAPPRDLGTSDLSASLSFRAHTRHVTSGVTVAF
                     DCTQCHRKPSDVLDIGHAFDDTPAKAEVGFSGGLSPEGNYDGGGTCSGLYCHGDGQGP
                     NGSIADGAPPRDCGSCHAGLDSGQSAWDAMGGLHAFHLSEGANCADCHSATTSDGRSI
                     AILALHVNGLQDVDFSIAGFAFDSQNRSCAGICHNHTHVASPWLDTLNRYHPPGYAAP
                     DVHGYDMQLQANDCRNCHAADLTGSLGPSCDSCHAPGWRSDCVFCHGGIDNAFGSPPR
                     DLAGVITNISFPGHTPHVAPGMARGFDCVQCHVKAVDVLSPGHAFDDTKGMAEVDLGG
                     GLSPQGTYASGDGCQSLYCHGNGQGDDGAIAIDAPAMGCDSCHAGLSSGAAGWSEMGG
                     LHSFHLGSAARCEDCHQATTIDSTSIADPELHVNALREVEFSLSGFTWDVVQRTCSGT
                     CHSHGHSALPWLDAGQRFHPADYALPQRHSPDMELQRIDCRDCHAADLTGNLGPSCDS
                     CHSEGWRSDCLFCHGADDNLTGAPPRDLGALPMSGSLAFLAHTVHVTQGISQAYDCNE
                     CHTKPTNVLSLEHAFDDTPGQGEVSFSMGLSAAGSYDGAGTCSSLYCHGNGRGSNGTI
                     VDGSAAMQCGSCHPGMGSTEGRWATMSGEHRKHLQEGFNCGNCHLDVTENGLSIRAPL
                     SHVDGAKQIRFTDTRINYNPATGRCSGSCHGEDHQSERW"
     gene            18855..>19175
                     /locus_tag="VFG22_02885"
     CDS             18855..>19175
                     /locus_tag="VFG22_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415216.1"
                     /translation="MKRKQVVLATVVAVAAAGLAGCRDDSAAIQALQELTTQLSQQVA
                     DQNDTLTSMTEKVQGCMKDLATTKGEALVITSKDATVDVPSLEGEASVASLEALKQAL
                     SETAE"
BASE COUNT         3625 a         5881 c         6014 g         3655 t
ORIGIN      
        1 ccgttgggga tcaaatcgaa cgcggagaag ccgagcatga ggccgagccc ggcgagaaga
       61 cgccgatcgg atgcccgtgg agcgtatttc agtcgtctca ctagagcgat gagagggaat
      121 aatagcaggc caaagctgct gacgaaaccg acgatccctt gatcaccgaa tcggatgatc
      181 cacgctccgt cacgtatcga taccggagac cgttcgcccg accagcgctc atagacgcaa
      241 gcccggcagt agctccccca tccgaaccaa gggcgctcca atgcccgatc gagcaaccgc
      301 gcttcgttct cgaaccgaaa cgtcatcgat tgggctcttt cctgtccgta atcatcctct
      361 gcccaggctt ggagaacctc cacggggatc aaatcgttcg ctcgaagcag aggatacacg
      421 aataggagcc cggcgagtgc ggcggcaacc aaggcttgga cgcggggcga agtgaacaac
      481 acgagtggca cggcggccat actatagagc aaggacgcga ggctcctcga gaggatgaga
      541 accacccaca gatacgacat gacccaggcc gcacggaatc gaagaattct aagcctggtc
      601 ttgaacaaag ccgcggctgc aatgagagct agtgtcgtga agatcgcgac ggcaagcccg
      661 tgcgccatga agaccatggg gcgatagctg tctccgcgca tgacctgcaa aaaggcatgc
      721 tgatggaatc cgtacaccca acggtgcaac tgtgggctga ggatcatctc gatgaactga
      781 agaggggagt agagcagcgc cgccaccacc agcgtagtga gcaggacccg aagatcccgc
      841 gatccccgga acatgacggc acccaaatag aaaggaaggg cgacgctgag cagagcgttg
      901 agcgccgcat gaacggcgtc gtacggaacg tgccctggga cggctcggtg gccgacgctg
      961 tacgggtcgg tattgagaaa aaccgtgaag accgacccga acacgaggag cccgacacag
     1021 actctgaacc accggctctt gggcgcggac gcgaagcgct cgcggtgaaa gacggctgcc
     1081 cagatgagaa tccacatcgg cacgatctcg agcttcgcga agtctggaag cccaggaggc
     1141 ttgaagaaca cgagctcggg gagcaggagc aagcccccca gaaagaggac tgcggtcgcc
     1201 ttggaaggtg gccagcgcca ggttgcgaaa tacgtgaccg ggacccagat gaagaatgcg
     1261 aggacgctta gaatgttgcc gtcggagtaa tattccacgc caaactcaca aattctcgcg
     1321 catcgacggc gcaaccaaca cgctgctcat cacgctttcg tgccggctcg cttgacctgc
     1381 atctgttttc gatcatgaca cgcccgcaag tttctcacca gcttgggtga accgtccgcg
     1441 gactccttaa attagtccgt ttacgtgtgc ggcgccgaga gactgtctat agtcatatcc
     1501 agtgaactta ctgacgatcc tagcggctct agcagcagcg attctcgcga tcccggctct
     1561 tatcttcttc gtggaatgct tcgccagcgt atttcttcgt gctgaaaagg aagtcgcgag
     1621 acccccgggc gtatctgtcc gagtgatggt tcccgcgcac gacgaagaag aaggcattgg
     1681 tgccatgttg gccgaagtgc aatcccaact cgcgcctgac gacagcgtct tggtcgtcgc
     1741 ggacaactgt accgatgaga ccgtacgact cgcgcgagcc gccggcgccg aggtgatcga
     1801 gcggaccgat gcggagcggc gtggaaaggg gtacgcgttg gcctacggca tcgagcactt
     1861 gaccccgagc cctccagacg tgctcatcgt catcgatgcc gactgccgcc tcaccccagg
     1921 tacgatcaac gctctcgtgc gaagcgctgt cgaaacgatg ggacccgtac aagccgatta
     1981 tgttctgcag gccatcgacc ggtccccgat ctcgatgatt tccgctttgg cttttctcgt
     2041 gcgcaatcgt gtgaggccgc gcgggctcct tcgcttgaac caaccctgtc acctcgatgg
     2101 ctcgggaatg gcgttcccgt ggagcgtggt gcgcaccgct cctggcttgg gcgcaaacat
     2161 cgtcgaagac ctcacgatgg ggatcgagct cgcgctgctt gggcacgagc ctcagctttg
     2221 catcgatgcc ggggtgcgaa gcgaccttcc gagctcccga ccggcgtcca tgcggcagcg
     2281 gcggcgttgg gagcacggac agatggccgc ccttttagag tacggtccac ggctcatccg
     2341 cgaaggggtc ttgcgtggaa ggcttggact catcgccctg ggagcggacc tgatggtgcc
     2401 accgttggca ctgctcgtgg gcctcttggt tctggtcttg ggcgcggccg gtgtcctcgt
     2461 tctcgtcggg ggctcaactg ttccggcctg gatcgccgtc gtggcgcttg gcctcattgg
     2521 gctcggggtt gccgtcggat gggcgcgtta tggacgaaag gagattccgc tgcggtacct
     2581 gctcctcgtt ccgatatacg tcgtttggaa gcttccactc tacctctcgt ttctcgtcgg
     2641 tcgccgcgag cgacggtggc gacgaaccga tcgctgaagg cgagaggcca ccgcgcgatg
     2701 cgctgctccc attccagccg tcggcactgc acccgtgtcc aggacgacgc tacggaatgt
     2761 tttaatgatc acaatgcatg ctctcgccaa ggcctcattc gaggtaaatc tacggccgcg
     2821 tcgagggact tccgattgct gcgcgtaagc cgacaggata cagttgcgac tcccttcgag
     2881 tctccacttc aaccaactca tgctatccga acgagatacg gcaaccactg cagggcgtgg
     2941 cttcttcaat gccggcacac ccttcatggg acgcgtcctg cgcgacgcag tcggcagagc
     3001 gtcgccgcct caggatcggg aagcgcacct cggcgcggcg gtggattggc tcaaacgggc
     3061 tcaggccgcg actcccgacg gcggagtgag ctacggctac tcgattcgcg ggggttggag
     3121 gccatcctac cgcgagactt cgggctacat cgccgtcacg cttttcgacc ttgccaagca
     3181 acgccaagac tctgaatccg cagccctcgc gcgcacggtc tcaaactggc tgtgcgaggt
     3241 tcaagagccc gatggatcga tcgcgaatcc gcagtatcgg ccccaccaag gcatcgtctt
     3301 cgacaccggt caagtgctgc tgggctatgt tcgcgcccac cgggaaacgg gggagagtcg
     3361 tttccgcgac gcggccgttc gtgcggcatc atggctcgca gatcacatcg acgaagacgg
     3421 ggcgtggcga agaaacactc acctcgaaac gcctcacacc tacaatacgc gcgtggcttg
     3481 gtcgatgctc gagtgtcacc aaatcgcttc ggatccagcc attgaagccg ctgcgagaaa
     3541 gaacctagac tgggcactcg ggcaagaaag agacgggtac tttcgtaact gcgccttcac
     3601 gcccagagcc gcgccgttca cgcatacgat cgcatacgcg attcgcggtt tcctcgagtc
     3661 ggctcgcctg ctaggcgacg catgctacga ggaggtcgcg gtgcgcgctg ccgacgccgt
     3721 gctcgagcac gtgcgggagg atggttggat cccagggcag atcgacgagg acggtcgatc
     3781 cgcggcgcgg tactgttgtc tgactggcaa ctgtcagctc gcgatcatct ggttcaagct
     3841 ctaccaatcc acggggtcgg agcggtactt ggatgcggca aaacgaagct tgggtttcgt
     3901 gatggagcac caagacctca gcacttcgaa tcctgctctt cgtggggcca tcaagggttc
     3961 ccacccgatt tggggatcgt acgctccgct ttcctatccg aattgggccg cgaagttctt
     4021 catcgatgcg gttctgctgt gggacgagat cgagccatga agtcggttcg catactcaac
     4081 ggtgaccttc acgccgccac cttggagggc actaccgact gggctcgcga gtggatccgt
     4141 agtggtcgac gcgggtacat ttgcacggtc aacgtggcaa ttctgatgat gatgaggtcg
     4201 gatcgtaggt tgcagggctt cgtcgatcgc gcgtcgctcg tggtcgcaga tgggcaggcg
     4261 ctggtttggg catcgcacct ccagggcaat ccccttccgg aacgggtcac cggtgtcgat
     4321 ctcgtggacg agctctgcgc gctcgccgcc aaggaaagct ttggcgtgta ctttctcggc
     4381 gccaagccta ccatcatcaa gacggcggcc aagcgcttgg ccgagcgcca ccgcggactc
     4441 gaaatttgcg gcatctcgga tggatacttc gacgcctccg aagcgccacg acgtgctgag
     4501 gcgattcgca agagcggcgc aagaatcctc atcgtgggca tgggtgtgcc cttgcaggag
     4561 gagttcatcg agaaccattg ggaccagctt ggagtcgagc tcgcgatacc ggtcgggggt
     4621 agcttcgacg taatcgcagg cgcggtccgt cgagccccgg tttggctgca acggattggg
     4681 atggagtggt tctttcgact cctccaagag ccccgccggc tttggaagcg atacttggtc
     4741 accaacaccc agttcatctt ccacttgctc aggagcgttg caggctcgga tcccggccgc
     4801 tcgtcgaact agcgcaaatg cctacgaaga cacggattct gatcgtcatg ggcacacgcc
     4861 cggaggtcat caagctggcg ccggtggtga tcggcgctcg tagtcgtccc gaagagttcg
     4921 atacgttcgt cgtcaccacg ggtcagcacc gagagatgct caatcaaatg ctcgcggtgt
     4981 ttcagatcgc cgtcgacctg gatctggaca tcatgaagcc caatcaggat ttgcgttaca
     5041 taacgaccgc ggcgctcacg ggcctgcacg acgtcgtagg gcaagtgaag ccggactggg
     5101 tcgtcgtgca aggcgacacg acgaccacac ttgccggcgc actggcagcc ttctaccaac
     5161 atgtgccagt cgctcacgta gaggccggcc ttcggacata cgacaagcgc caaccctttc
     5221 ccgaagagat gaaccgaagg ctcaccacgc agatcgccga cgcgcacttc gcgccgacga
     5281 cgctgtcgcg cgacagactg ctgcgtgaag gggtgagcga agaagcgatt tgggtgaccg
     5341 gcaacacggg catcgacgcg ctgtttctga ttctcggatc gaacgccggg gagacagctc
     5401 cccaacccac gggtgagcgg cgtcttctgg tcacggccca tcgccgcgaa aatcaaggtg
     5461 agccgatgcg gcaaatgtgc cgagccttgc tgcgcctcgt ggatgaattc tcagatcttc
     5521 gcgtggtcta tccggtccat ctgagccctg cggtgagaga cgtcatcttc cccctactgg
     5581 gcaaccattc tcgcgtcgag ctagtcgagc ccctcgaata tcgtcgattc gtcgaagaga
     5641 tgaggcgtgc ccacatcatt ctgaccgatt cgggtgggat tcaagaggaa gcgccttcac
     5701 tcggaaagcc ggtcctggtc atgcgagaga ccaccgagag gcctgagggg gtcgacgctg
     5761 ggacggctcg actgataggt accgacgaag agcggatcta ctcggagacc gcgcgtctgc
     5821 tcagcgacga cgacgcctac acggagatgg ctcaagcgcg caatccatac ggcgacggac
     5881 gagcatctga gaggatcctg accgccttgg ccaaagggta tcggcaacgt tgacgggaaa
     5941 cgtcgccgcg cccgtattgg gggccgcgcg catcccgctg cttggcgtcg agaccgttcg
     6001 tgcttctgcc ttggcggtgc cttatgcttg taacgatgct ttttggaccg gccgcctgct
     6061 ttcaccatga ccataccttc tgattcctcc ccgattcgcg tcggcgtcgc cgggaccggg
     6121 ttcattgcgc gccgcttgtt tgattcctgt cgattgatgg acgacctaca ggtctcctcc
     6181 gctctcaccc gccgcgcgct cgacagcatc aaagacatgc cctcggacgt gcagctcacg
     6241 caatcgacgc aagagctaat cgacggttct gatctcgtcg tcgagtgctc gggtgacccc
     6301 atacgtgccg ccgaagtcgt ccgagacacg ttggcggcag gtttgccagt cgtcacgatg
     6361 gactcggagt tccatgtcac ttgtggctca cacttcgtgg gcaaaggcta tctctccgag
     6421 gcgaacggcg accagccagg cgtgcaagcg caacttcatg atgagctcgt cggcatgggt
     6481 ttcgagcccc tggtgtacgg cagcatgaaa ggattcttga atccgaatcc gagcctcaaa
     6541 gacatggcgt actggtcaga aaggcaaggc tttcgagtcc agcaaacgac gtcttttacc
     6601 gacggcacga aagtgcagat cgagcaagcg ttcgttgcca acgcctttgg cgctggcatt
     6661 gcgaaacccg gcttgttggc cgtccaaaat gacagcgtcg aagaggccgc acgcgctctc
     6721 gcagaagctg cgctcgagct cggccatccg attgcggact tcaccgtcgt gcccggtcag
     6781 gcgccagggg tattcgtgat cgccaccatc gccgaatcac agcgtcccgt cctggctgca
     6841 ctcaaaatgg gggacggccc ttactacacg ttcgttcggc cctatcacct atgcgccctc
     6901 gagatcccca acacaattcg gcgtgcgatg gaggggcaac cgccactctt gaacaactcg
     6961 accgctccga gcattggcgt cgctgcagtg gcaaagagga gcctccgccc gggggagcac
     7021 atcgagacgg gaatcggcag tttccaggtg cgcggtacgg ccgtgcgctt ccgcgacgag
     7081 ccggaccacc ttccgatcgg cctcctttac ggtgccacgg tgacgcgtcc ggtagagccg
     7141 gaccagatgc tcacgctaga cgacgtggat ttacccgaca gctatgcgcg ggacatcacc
     7201 ttggggctga ttcgagacgc gtgcggctaa aaaaagatcg catttcacgc ctttttccaa
     7261 tttctcgggt tccggcctcg gggtctcggc cctaggtggg agcgctgcag gagtgcaatc
     7321 ttcgtgcgct cgagcacatc gattcacgcg gcgcacttac gaccgatgac tcacttcgac
     7381 gcacggcagg ctgacatcga tcttctcaat gcgtttcttt gtcgcgagcg tagcgcgcgc
     7441 gaggcctatg gggtgctgct cgaagccagc tcggacgagc agatacgccg ggcgcttgcc
     7501 cgtcttcgaa actctcatca agagcgaatc aacgagttgg aggggcgtat ccgagctctc
     7561 ggggggatcc cccgtgcaca atcgagcctg tggcgcaccc tgttgagggc cagggagcag
     7621 agcgccggaa ggctcaacaa aggcactgtg atcttcacct tggaggatta ttcgagtcgt
     7681 attcttcgcg cctatcgtcg cagcttgacc gacctctctc ccgaatcgcg gaaattcatc
     7741 gagcgcaaga ttctcccgga aagccagatc gtgcaccagt ccgtcatctc gctccagaag
     7801 gcgctctaac ccgcagcggc gaggcccttg caggcgcgct cccgttcgag gcggcccttc
     7861 tcgacggact gcgtttcggg cgagaaaagc cgcgtgccga tggacatgcg ttcgagcggc
     7921 gaagcatgca tactgcggcc cgatgcttcc atttgcacct gtgtttcgcc gggagagctg
     7981 ctgaatgtct ccgctcggtc tcgggttgag cgtcctgatc gggatctcgc tgggtttttt
     8041 tggcggcggt ggctcgattc tcacggtgcc gctgcttgtt tacgtgttcg gcctcgatcc
     8101 gaagactgcg atcgcaagct cgctgctcgt ggtgggcgcg gccagcacgt ccggtgcgct
     8161 ccaccactgg cgggcgggca acgtcgacgt tcgaacgggg cttctctttg gagcggcggg
     8221 gatgacgggc gcctacctcg gcggccgcgt aggggcgttc ctcgatggga cgctgctgct
     8281 gctgctcttc gcatgcatga tggtgttgac gtcggtgggg atgtggcagg gacgccgcgc
     8341 gcgaagcccc ggcggtgcaa ccaaacgagc gacgggccgg ctgctcttgc agggcctagc
     8401 cgtcggaagc ttcactggcc tcgtgggggc cggcggcggg ttcttgatcg tcccggcgct
     8461 gacgctttgg gcgggcctat cgatgcatgc ggcggtcggg acgtcgttgt tcatcatcgt
     8521 gctcaacacg atggcgggct tcctcgggta tctgagccac gtgcaggtcg actacacgct
     8581 catcggcgcg gtgacggtca tggcgattgg aggctcgttc atcgggtcgc gtctcgccag
     8641 tcaggtcaac ccggcgtcgc ttcggcgggc gttcgcggcg ttcgtgctca cgatggcgat
     8701 cttcattttg gtgcgcgagg cggatcagtg gatcaccacc gcacgcgacg cgcttcccgg
     8761 cacgacgccg cagatcgtct tcgctctgct gatgctaggc atcgggatcg cggccggccg
     8821 cgtgaccagg cgcgcggccc gtgagccgac ggccgatcga ctcttctcag acggcggcgg
     8881 aatctgatga cctgaccgcc accctcgtca tcggatccat gacgatcgcc tcaacgggcg
     8941 agctgccgcg cgaccaatac ctcggtgcag ataccgagcg tgcagacgac ttcgcggtga
     9001 aggatctgcc agtcactgcc gatgaattcc tcgaggtgga gaggccggca tccaccaacg
     9061 agccttgggc tgaacgagtg cattgcgctc caagcgcggc aaacgagcct cgatggaagc
     9121 gtctcaccgg aagagtcgga gagccgtccg ttcgctgcat gcgagcgcgg tccccccagg
     9181 gcgcgacccg tccatgggcc aggtcgatca tggtttcgct gatgtctagc ccgaggtact
     9241 tggagtcttc tgggagaagc tcttcgagga ggcgcgccgc gagcctgccc gtcccgcagc
     9301 cgagctcgac gaccgcgcag gcggtttgga agctcgcgta ttcgagaagc acctcaatcg
     9361 caggatcctc gtagattcgt tgcaggtcct gctttggacc gaactggtcg tcaaaggcct
     9421 ttgcttcctc gtgggagagg actcgctggg ctccgctgcg tggctttgcg acgtgcatcc
     9481 tcagcggagc gtggcaccgt cgtgcggacg agcgcaagcg tcgcgtcgtc ggctcgccgg
     9541 ggcttcagaa cgcgcgagtt tcgccgatgc gcagtaacca cccagcctcg tctccgagag
     9601 cactctcagt tgcggccttc ttgaagtgtt tggcgggctc gtccaaaggc tcatccgaaa
     9661 gcttgaaggt gccgtagtgc atcgcgaccg ccgcgcgagc gcgaaggtcg atggccgctt
     9721 gaacggcctc ttcgggattc atgtgtgaag ccttcatcat ctccgccggc tcgtaggcgc
     9781 cgatcggaac ggctgcgagg tcgaacgggc caagcgcctc ctcaatgcgg gcgaatccat
     9841 cgaagtaacc cgtgtccccc gcgaagaaga accttcgctc tggcgcaagc accgcccagg
     9901 atgaccagag tgcttcgcgg tcatcgccga tgccccgctt gctccagtgc tgagccggaa
     9961 ggcagtagac gcgcaccccc ccgagctcga cggactggcc ccagtcgagc tcttcgacgt
    10021 tttcgactcc gttgtcgcgg aggagctttc cgttctcgag gggaacgaag aagcgcgtgt
    10081 tcggatcccg ctccgatagc gcgatgaggc ttgcaaggtc gaggtggtcg tagtgattgt
    10141 gggagaccac cacgaagtcg atcggcggaa ggtcgtcgat cgcgacgccg ggctccacga
    10201 ttcgctgcgg ccccgcgaag gagagcgggc ttgcccgatc cgaccagatc ggatcggtga
    10261 ggaacgtgag gtgccccatt tgaacgagga gcgtcgcgtg accaatccag gtcaccgtgg
    10321 gctcgctgtg cttcgcgttc tcgcgcagcc aggcgccatc gttagggacc cgatcgaggg
    10381 cgctcggggt gtccgtgaaa gagctggcga tgcgtcgaac gaagaagggc agggtcaccg
    10441 agacgccaac ctcgggcatg tgcccggcaa aattcatgaa tcgaccgtca cctccacgag
    10501 gggcggggcc gagcgcgtcc tcgaccgggg atgcagcggc tggtgacacc agcgccagcg
    10561 ccaggaagag cagggcgcgg ctcggagagt atcgaccagt gagcatgagc ttcgcttcat
    10621 ctggggcttc tgccaccccg cagcaaggga gattcaaccc ggtcgctcat ccatgcacat
    10681 tctcggggac gaaatcgatc ttcgaagcta ccatccgctc catgatgcat ccgtccaccg
    10741 agctccggca catcgacccc gagctcggat ttggtgtctt cgcgacatca cgactgccaa
    10801 agggcaccat cacgtacgtg tgggacccgc tcgaaatcca gatcatgccc gacgatccca
    10861 gcctccaaga gccggcattg cgcgaatcga tcgatcgctt ctcctacatc gactctcacg
    10921 gcgttcgaat cgtgagctgg gatcacgcca agtatgtgaa ccattgctgc caatccaaca
    10981 cgatgagtac ggcatacgga ttcgagattg ccgtgcgcga catcgaagct ggggaacagg
    11041 tcaccgacga atacggcatg ttcaacctgc gcgatcccat gaagctccgg tgttcaagcg
    11101 gcccctgtcg cggtgaggta cgagccgcgg atctcgaacg gttctgcctc gtttgggatg
    11161 agcagatcaa ggcggctcta gagctcgtgg agcaggtcca ccaacctttg tggccgctca
    11221 tcgatccgct gttggccacc gaggtggagc gctacgcccg gtggggtgaa ggttatcgct
    11281 ccgtccgaga gctcggcgaa atcaaagcct ccgatcgcgc gcattctggg taactcaatc
    11341 tttggttgcg agctgccgaa tgtcgagagg cgctttcgga aagagctcga tcgccgctgc
    11401 tgcaacggat atggctccgc cgctcgactc gatggctttc agcacccgcg cgctgagcag
    11461 atgcttcgtg gcccgtcgcc ggtcgaattg aacggtgtat cgaatcgtga acttcaccca
    11521 gttttcatcg aagccgaacg tgaccatcgg aacggtgctg gcatcttcga ggggaaagcg
    11581 tctttgcatg cgtgcccaga tggtcgtcgc ctgctcggca tatgctccgc agacctcgtt
    11641 cgccgcttcg gtgagcgcgg cttgagccat gtcgagatca ctcccgaacc gaattggcac
    11701 ggtgatctca tcccacaaga aaggaaaatc cccggagtag ttcacgactg gctctttgaa
    11761 gacgaagctg ttcgcaacgc gcaccattcg gccgttgtag agatcgccat cgacccactg
    11821 gccgacctct gccagggtcg tgcggagcac tccgatgtcg atgacgtcac ctttgatgcc
    11881 accgagttga atacggtcgc cgacggtgta gaagcttccg aacgaaaccg ccgcccaacc
    11941 agcgacgctg acgatgacct cctgaagagc gaacgcgatc cctgcgcccg caacaccgaa
    12001 aaccacactc atgcggccta accggtcgct gaacatcgcg agcacgacca gggcgccgag
    12061 caagtaaccg atgaatccga tcgcctttcg agctcggtag cggacagact tgtcttcgat
    12121 gtaccgcgtt gcgactcgct tgatcacttg gacaatggcg accacgatca cgaccgcgat
    12181 cagcgtgcct gctagcttcg aaatcgccgg gtcgtccagc aatttcatcg cgttttgaat
    12241 cgccttttcc atagtctatt tctcccgcgc ggcgtcgggc gtcaggagct cacgtgctca
    12301 ggcgctcggc ttggtgcttc tcgatcaact cttgggcgat ccgatcggga acggggtcgt
    12361 aatggctgag ctccatcgtg aagctcccgc gtcccttggt catgctcttc agcgccggct
    12421 cgtactccat catgtcggcc agcggaacag tagctttgac gatcgcggtg ccggtgcttt
    12481 tgacgtcggt gacgaagatt ttaccgcggc ggctcgaaag gtcgcctgtg atggtgccca
    12541 cgtggtcgcc gggtgtagtg acctcgacgt gcacgacagg ttcgagaatc tgcggccggg
    12601 ccttgaggaa ggcttcctta aaggcgaact tcccggcggt ccggaacgcg atttccttgc
    12661 tgtcgactgc gtgagtcttc ccgtcgaata cggtgacgcg cacatcttcg atcggataac
    12721 cggcgacgat gccccgccgc atcatgtcgc ggacgccctt ctcgatggcc ggcaagaaga
    12781 ctttggggat gacgccaccg acgattttgt cgacgaactc gaagccctgt cctcgctcga
    12841 gcggctccac tcgaaggctg acctcgccga actggcccgc accgccgctc tgcttcttgt
    12901 gccggtactg gccttcggcc tcgcccatga ccgtttcgtg gtacgcgatc ttgggtggct
    12961 tggtttcgac ttcgacgccc tggttcttga tgcgctcgat gaccaggttc agatgcattt
    13021 cccctaggcc gtgaatgact agctcgcggg tttcgagctc gaagtgcgcc tcgaaggtcg
    13081 ggtcttcctc gcgaatgcgc gcaagcactt cggcgatctt gtcctcgtcg ctgcgggtct
    13141 tcgggacgac cgccaggccg tgcaagggtt tcgggtacgg cagtggcttg tggtgcacca
    13201 ggtcgagcgc atggtcgtcg tggagcacgt ctccgaggtg cagctcgtcg agcttcgcga
    13261 tgacgcccac gtcacccgcg atgaccgaat ggatcgcggt gcgctgcttg ccttgcgtcc
    13321 ggaacagatg accaatgcga atcggcctct ttccatcacc gacgaagagc tggtctccgg
    13381 cgttgagctt tccctggtaa acccggaaga agcagagctt cccgaggtac ggatcggttg
    13441 cgacgtgaaa gacgcgggcg agcaggggct tgtcggcatc atccgagtag acgaacggct
    13501 tctcgtctgc gccttcgccg atgaagaacg gacgtgggtt gccctcggga gggcttggga
    13561 agtagcgcac gatgatgtcc agcaactcgc cgacgcccgc tccgctcaga gcatccgtga
    13621 aacagacggg gatcacgtgc gccttatcca tggcgcgctc gaacggctcg tgaagcgcct
    13681 cgtagttggg ctcttcgccc tcgaggtatc gttccatgag tgcatcgtcg atctcgacga
    13741 cttgatcgag aagctccgtg tggaagccgg caaccgggcc gaggtcgctc tcacccgtcc
    13801 cgttcagcaa gcaacctatg acggcttcac ctttgttcgc cggcaggttg atgggaagac
    13861 aaccgcggcc gaacgcttct tggagctcgg agagaaacgc ccccagacgg gagacgccct
    13921 ggccgatgtg gctcaccacg atggcgatcg gaaggtcgag gctgcgcgcg agcttcacga
    13981 gacgccggct ggcgctgtcg acgccgacct ccggatccat gaccagcatc atcgtctcgg
    14041 cggcagggag acaagcaatg gcctggccga tgaaatctgg cgcgcccgga aggtcgagca
    14101 ggttgatgcg cttcccctgg tgtgttgcgt gcatcaaggt cgaatacacc gagtactggt
    14161 gcgcttgctc ttccagctcg aagtcgctgg tcgtcgtgcc gccgtcgacc gatccctgcc
    14221 ggctcgtttc accggcgcga aacagaagcg cttccgcgag cgtggttttt ccgctcgagc
    14281 gtgagccagc gaggagaacg ttgtgaaaat ccggggttcc gaagtcgggc atgggaggat
    14341 atgaagcaag tcttgggcgc cgctgcaagt cgagccggtt tgcggcatac tcccggccta
    14401 ggttcccagc tcgaaagaag gaaaactcat ggctgaactc aacaggctcg acccggtcac
    14461 cctggacttc ttcgatacgg cgcccctgcg tgtgcagtgc acgatgttgg ccaagtgcac
    14521 tcccgaacga ctcttcgaga ccctccgcgg cgataccgtc tggaccgaat gggcaggcgt
    14581 catcaagggc gtggactgga cctccgagaa accctacggg aagaacgcga ctcgcgatgt
    14641 ctcattagtg ggcggaatgg tggtgaagga gctcttcttt cactgggaag agaacgcgcg
    14701 tatcgcgttc tatgtcaccg aatcgaccat ccccggcctt tcgaagttcg cagaggacta
    14761 cagagtcgaa cgcgtcggcc cgaacgaaac gaagctgagc tggacggtgg cgatcgagaa
    14821 ccagggtctc atgcgccacc tgaattccgt caccgcgcct gtcatgaagc tcgtattcca
    14881 gggctggctc aagaagtaca agaagattct cgagactccc gtggcgaagc ccgcggccca
    14941 gcccattgcg gcccctcagc cacgcgctta gcggcctagc ggtttcgtat tcgatgccga
    15001 gggcccaaat cggacccaag aaaattgagc cggtttagtg ctcagcggtc actgggaaga
    15061 cacggcggag agcggtgcga cgagcgttcc ggcttcgcag gataggtcgc atgtcccgaa
    15121 gatctgcact cgacccgctg cgttccaatt ccggtggtga ccgtgatggc gacggtgccg
    15181 ggaccgcagc tgcaaatatt tcgcaagccc cttcgcgagt gcagatgttg caagcagctg
    15241 gcctcgagga aaggacgctg cgcacagttc tgggcgaaag agcgaagaat cagatccttt
    15301 ggtacagcac ttgcttgccc ttcatcgatg tgatcatgcg ccgcttttcc cgctcgtgtc
    15361 gtggcttatc catcgcggtt ctctgtgcgt ccgtcctcat cggttgtggc acggatgggg
    15421 gagagattgg ccagcccgat ggcggcttgc ccgatggcgg cgagggcggc gtcggtggta
    15481 gcggtggtag cgctggtgtc ggcggaatag ccggctctgg cggatcggcc ggcacgggcg
    15541 tctccgggcg cttccaccgg tccgactacg agaaaccgga gctccacggt tcggatctca
    15601 ggctcggggt cttggattgc aggttatgcc acggcaagga tctaagcgga ggcggggaca
    15661 tcgaacccag ctgcgatact tgccatacgc ccgcagagcc agaggcgtgg cgagcggagt
    15721 gtgtcttttg tcatggcggc gtggaggacc tgagcggggc ccctccgaag aacctcgacg
    15781 gtaccctcac cgtcgccgcg tccgcgttcg tgccgcatca cgcacacgtc cgcactgcaa
    15841 tgagcgagcc gctcgattgc atcgagtgtc acgtcaacgt taccgacgtc cttacgcagg
    15901 gccacgtgtt cgacgatacg ccggctgtcg ccgaggtgga tctgggcggc ggtcgtagtc
    15961 ctcgcggaat gtacgatccg gttgatggct gcgcgagctt gtactgccac ggcaacgggc
    16021 agggtgacaa tggcagcctc ggtgcggccg atccacccat gacttgtgaa agctgccacg
    16081 ccggcgcgag tagcccatcg gtcgagctcg ccaaaatgag cggtcgacac ggtttccaca
    16141 tttcgatcgg tgccggatgt caagactgcc atcaggcaac taccagcgac gggctgagca
    16201 tcacctcgcc gtcacttcac atcaacgggc agcgcgacaa ccagttctcc gcacccgggt
    16261 tctcctacga tcaaggcacg caaggctgca ccggtacctg tcacggatat ccacacagcg
    16321 cgcggccctg gatcggctcg agccagggct ttcatccggt aggctttgcg gatcccgaga
    16381 tgcacagccc cgcgatggag cttcaacgga tggactgtcg cacatgtcat ggggccgacc
    16441 tcagcggagc cgccggccca agctgcgaca gctgccacac ggtgggttgg cgccgcaact
    16501 gcttgttctg tcatggcgcg ggagacacgc tcaccggcgc gcccccaagg gatctcggaa
    16561 cctccgatct atcggcgtct ctgagcttcc gcgcccatac gaggcacgtg acctccgggg
    16621 tcaccgtcgc cttcgactgc acgcagtgtc atcgcaagcc gagcgacgtg ctcgacattg
    16681 ggcatgcgtt cgacgacacc ccagccaagg cagaggttgg ctttagcgga gggctcagcc
    16741 cagaaggcaa ctacgacggc ggcggaacct gctcggggct ctactgccat ggggacggcc
    16801 aagggcccaa tggcagcatc gcagacggcg cgcctccccg ggattgtggc agctgtcacg
    16861 caggactcga cagcgggcag tcggcctggg atgcgatggg aggcctccac gctttccacc
    16921 tgagcgaggg tgccaactgc gccgattgtc actcggcgac caccagcgat gggcgctcaa
    16981 ttgcgatcct ggcgctgcac gtcaatgggc tgcaggacgt ggacttctcg atcgctgggt
    17041 tcgccttcga ttcccaaaac cgaagctgcg cggggatctg tcacaaccac acgcacgtcg
    17101 cttcgccctg gctcgacacg ctcaatcggt atcacccgcc cggctatgcc gcgcccgacg
    17161 ttcacggtta cgacatgcag ttgcaggcga acgattgccg caactgccac gccgccgacc
    17221 taacgggcag cctcgggccg agctgcgata gctgtcatgc gcctggttgg cgatcggact
    17281 gcgtcttttg ccacggcggc atcgacaacg cctttggctc gccaccgcgc gatcttgccg
    17341 gcgtcatcac caacatcagc ttccccgggc ataccccgca cgtcgcgcct gggatggccc
    17401 gcggcttcga ttgcgtccag tgccacgtca aagcggtgga tgtactgagc cctggtcacg
    17461 ccttcgacga caccaaaggg atggcagagg tcgacctcgg cggcggcctg agcccgcagg
    17521 gcacctacgc tagcggcgac ggatgccaga gcctctactg tcatggaaat ggccaaggcg
    17581 acgatggcgc gattgcaatc gatgcgccgg ccatgggttg tgacagctgc catgccggtc
    17641 tttcgagtgg cgccgcaggt tggagcgaga tgggcgggct tcattcgttc catctcggct
    17701 cggctgcacg ctgcgaggat tgtcatcagg caacgacgat cgacagcact tcgatcgccg
    17761 atcccgaact tcacgtcaac gccctccggg aggtcgaatt tagcttgtcc ggcttcactt
    17821 gggacgtggt ccagcgcacc tgcagcggga cctgtcacag tcacggccac agcgcgcttc
    17881 cctggctcga tgcggggcag cgttttcatc cggcagacta cgcgctgccg caaaggcata
    17941 gccccgacat ggagctccag cgcatcgatt gccgtgactg ccatgccgcc gatcttaccg
    18001 gcaatctcgg tccgagctgc gatagctgtc acagcgaggg ctggcgatcg gactgcctat
    18061 tctgccacgg cgccgatgac aatctgaccg gggcaccgcc tcgcgatctc ggtgcgcttc
    18121 cgatgtcggg ctcgctcgcg ttcctggctc acaccgtgca cgtcacgcag gggattagtc
    18181 aggcctacga ttgcaacgag tgtcacacta agcccacgaa cgtgctgagc ttggagcacg
    18241 ccttcgacga cacacccggc caaggggagg tgagcttttc gatgggcttg agcgcggccg
    18301 gtagctacga tggagcgggc acctgcagca gcttgtactg ccacggcaac ggtcggggaa
    18361 gtaacggtac catcgtggat ggaagcgcag cgatgcaatg cggctcttgt catcccggca
    18421 tgggtagcac cgaaggccgc tgggcaacga tgagcggaga gcatcgcaag catctgcagg
    18481 agggcttcaa ttgcggaaat tgtcatctgg acgtgacaga gaatgggctt tcgattcgcg
    18541 cgccgctttc gcatgtcgac ggggccaagc agattcgctt cacggacacc cggatcaact
    18601 acaatccggc aaccggccga tgctctggaa gctgtcatgg cgaggatcac caaagcgagc
    18661 gctggtagcg ccccttctcc gcagagccgc ggacgccgct gccttccgac atcatgtttg
    18721 ggccggcagg ttgccaggcg tccaggcgtc gcgctatgag tgcctggcag tctgggtcgc
    18781 catcaagggg tgagagtcgg cggcgtggga ccgcctttag gcgtgtgctc gcagcttgct
    18841 gaatggaggt actagtgaag cgcaagcaag tagttttggc cacggtggtt gccgtcgccg
    18901 cggccgggtt ggcgggatgc agggacgaca gtgccgcgat ccaggctctg caggaattga
    18961 ccacgcagct ttcacagcaa gtggccgacc aaaacgatac cctgaccagc atgactgaga
    19021 aggtgcaggg ctgcatgaaa gacctcgcca cgaccaaggg cgaggcgctg gtcatcacgt
    19081 ctaaggacgc cacggtggat gtcccctctc tcgagggcga agcgagcgtt gcttctctag
    19141 aggcgctcaa acaagcgctc agcgagacgg cggaa
//
LOCUS       DASRZF010000064        11829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3141208, whole genome shotgun sequence.
ACCESSION   DASRZF010000064 DASRZF010000000
VERSION     DASRZF010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 11829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11829
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3141208"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VFG22_02890"
     CDS             <1..370
                     /locus_tag="VFG22_02890"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET6415217.1"
                     /translation="SEDEKTKGDSGDLQFFEAEGEGPPPASIRKAAFSISKVGAVYPE
                     LVEDGDGYHIVMLTGKRAALTRTYEQSKRAIRHKLTRQRKDAAMEALTERLRQMIEVD
                     VDYSVLQDIKVHVDDLPPKP"
     gene            367..1308
                     /locus_tag="VFG22_02895"
     CDS             367..1308
                     /locus_tag="VFG22_02895"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HET6415218.1"
                     /translation="MRGVLAVAALVLALAAGAADADVVERVVATVDNQAIFLSDLRTR
                     AVPFLPQVADATTETERKARLKELYEELLNYLIDEQLIRQLATSSGVRITDADVSNAI
                     ENLRLQNNMTEQQFQQALVQQGFTEAQYRQDLKRQLLRLKVLNERVRSRVNVTEEEVR
                     ARYEQRARGEGNAIRFHVSHVVVPVADGASAIQLAAKRQEAASLRETLTPENFEARAE
                     ALGGGDLGWLSEGDLPEALEVALAPLNPGDISDPVRGGAGFHIFFLKDRQIGDDFPTY
                     DEMKQELYREMLDAAMIKQEKVFLDEVRRKAVINRML"
     gene            1365..2147
                     /locus_tag="VFG22_02900"
     CDS             1365..2147
                     /locus_tag="VFG22_02900"
                     /inference="COORDINATES: protein motif:HMM:NF004781.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HET6415219.1"
                     /translation="MSGELRIEKDGSIAQLVFDHEKRRNAITVDMWEAIPSAVRTVQD
                     DSEIRVVIVRGAGSEAFVSGADISEFEKTRAGASGREYDAMTARAFAALQGLDKPLVA
                     LIHGFCIGGGAAIALTADLRYAADDALFAVPPARLGLGYHTAGIRALIRTVGFSETAD
                     LLFTARRVPADEARHIGLVNEVFAKDVLDERVEAIAHRIAENAPLTIRSAKISLQELA
                     KLETERDSPRIAESIERCYQSEDFREGVRAFLEKRKPRFTGR"
     gene            2195..3202
                     /locus_tag="VFG22_02905"
     CDS             2195..3202
                     /locus_tag="VFG22_02905"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET6415220.1"
                     /translation="MLEKQSTGLLAFWRDYERKTYVKAAVLADRLGYDSFWLPEVWGY
                     EVFSLLTEIALKTKRIKVGTGIVNVFSRSPALIAMQAATLDEISGGRFILGIGTSGKN
                     VIEGLHGRDFEKPLTQTQDVIRVVRTMLDGRPLSEAGAKLRQYRPFTLDFEPTRKRIP
                     IYVAALKQKSITSVGEMADGWMPIFWPYNRLEDGRRWIAEGAAKAGRDPSEIATAPFT
                     TVIPIPGKGASAKARELISFYIGGMGDYYKELLSGFGYAEECNEIENLYRDKATRKMA
                     PDAVTDDMIEALTISGDPLHCRREIARRRELGMEQPIINLPPGMPWPGLAAFITALAR
                     A"
     gene            complement(3458..3820)
                     /locus_tag="VFG22_02910"
     CDS             complement(3458..3820)
                     /locus_tag="VFG22_02910"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET6415221.1"
                     /translation="MTAEVSSGDEEDSAEAETSPEERETDAGPRVSRMRVGGEDILVL
                     SVPLPKLRYPIDTTPAEHAIIDAVLKGLTIKEIAEQRNASLRTVTTQLGAIFRKAGVN
                     SQAELIASMTDGDAPKSK"
     gene            complement(3894..5036)
                     /locus_tag="VFG22_02915"
     CDS             complement(3894..5036)
                     /locus_tag="VFG22_02915"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6415222.1"
                     /translation="MERSLFSEEHEIFRRNFRQFVETRVVPEQAKWRSQGVVDREIWL
                     EAGESGFLCPWMEEEYGGAGGDFLHSVVVMEELCRAYESGWAASLHSDIIVPYIYRFG
                     SQEQKAKWLPACASGEAVTALAMTEPGTGSDLAGIVTTAKRDGDEYVINGAKTFISNG
                     MSCDLAVVAARTENSDDPHRSVSLFLVEADRPGFIKSRRLEKMGMESQDTAELAFDEC
                     RVPALNLLGQEGAGFMMLMQNLQQERLCVGIMSQASAEKVLEDAIAYTQERKAFGRSI
                     SKFQNTQFTLAQCATEVEVGRAFLDAVVAQHVAGKYLVKECSMSKLWHSEMQGRVVDA
                     CLQLYGGYGYMLEYPISRAYMDARVQRIYAGTNEIMKVIIAKQMGL"
     gene            5150..6463
                     /locus_tag="VFG22_02920"
     CDS             5150..6463
                     /locus_tag="VFG22_02920"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET6415223.1"
                     /translation="MRRRPARFSLLLVLFAAIPATALASGDGHGLSYDIWLVLAGMIL
                     AAKLGGEVAVRFNQPAVLGELCVGILLGNLYLVGIETFHGAAELGPIEFLAEFGVVLL
                     LFEVGLESTLTEMRAVAPTSGLVAIIGVVAPMLLGYGVSLWLLPEASFSLHLFIGATL
                     CATSVGITARVLKDLDAMGLPATRVILGAAVIDDVLGLIVLAVVASIAEFGAVPGPLD
                     LSKIIGLAAGFLIGALLLGAYVMPGVFRMASKLRTQDVLAAVAIGLCLLLAGISGAAG
                     LAPIVGAFAAGLILDEVHVRPFGRKSAHDLNEIIGPIVAVMAPIFFVRTGMMVDLGGI
                     GTEPLILAAALTAVAIIGKLISGLGVRGATADKLTVGIGMVPRGEVGLIFANVGAGIT
                     FEGKPLIAAGVYAAIVLMVMATTVVTPPWLAHRIRQVSRKLEDVA"
     gene            6453..10055
                     /gene="mfd"
                     /locus_tag="VFG22_02925"
     CDS             6453..10055
                     /gene="mfd"
                     /locus_tag="VFG22_02925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HET6415224.1"
                     /translation="MSLEATLANSNASIRDLVASLGANERVEAASVPRGALSWAIAKA
                     AAATSQRRFVLVTSDLEEAYRYESNLRFLLADDDGDVLLFTAADTSPLLDVVPDRRAE
                     MQRMAVLAQLAEERPWRVLIVPATALLRRVPPADHVKSGLLGIEFGDRIERDELVRRL
                     TELGYLRVPLVEDRGTFSARGALIDVYGPDASLPVRIELDDDLIVRIRRFDPEDQKSA
                     DEIESTQLAAAREVPDTAKEISRAKTAVRELCDEMNMPTLRARELIDELDRGSGALIS
                     NALLPAYFEALDTLFDYIPSDARLVLADPAAIAESVRTEERQAKDEHAARRDRPTFEL
                     EKYLLSEAELANRLRGYSPLVVHRLAVHGAAAEDEGPIVAAFTPPANELMRVDASDQA
                     DLISKLRAQKGHRERGLQPLADQILQWTEEGLKVSLIGRAQHQADRLIDLLRSYGVAA
                     NVLDGILLGELRDGFVLWSEGIAYVTEEEIFGSRVRQRRSKRQSRRQQQRFLEDLREL
                     NPGDFVVHVDHGVGKYLGLQHKALSLTALDRLHGRTPQTVEVMVVEYAGSDKLFVPVT
                     RLGVIQKFKGGEGHQPKLDRLGGTTFAAKKGKVQKAVQQMAEELLKLYAERSAARREP
                     LEPADTAYAEFEATFPFEETRDQEKAINDVMADLEEPQPTDRLVCGDVGFGKTEVALR
                     AAFRVAMGGGQVAVLCPTTVLAQQHYRTFSARLDGYPLRVEVLSRFVSRTKQIEVLGA
                     LKDGKVDVVIGTHRLLSKDVHFAKLGLLVVDEEQRFGVTHKERIKKLRTNVDVITLSA
                     TPIPRTLQLAVGGMRNLSLITTAPQDRRAVRTFVCRWDDHLIKEAVERELSRGGQVFF
                     VYNRIEGLYERAQRLQELLPNARIAVAHGQMKPALLDKTMTDFVEGAYDVLCSTAIVE
                     SGLDIPRANTILVDRADTLGLAQLYQLRGRVGRSDQRAYCYLITPPPNQMSEESRVRM
                     EALERFSGLGAGFRVATLDMELRGAGNLLGSEQSGNAELVGFDMFVQMLDQAVSELKG
                     DHVQQEVDPELSIELEHYLPEDYIDDIGLRLSLYRRFATAIDEQAIEDLAEEMEQRFG
                     APPNQARDFVRVMSLKPPLRELRALGCEGELKRVTLHLREDTPLDPAKLMPLVATPGA
                     GWSLSPDMKLTRRYRDDESGDAVDRVRSLLRELEPMRSAAAQQQKDGSDDVTSR"
     gene            10039..10680
                     /locus_tag="VFG22_02930"
     CDS             10039..10680
                     /locus_tag="VFG22_02930"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET6415225.1"
                     /translation="MSLPDELQRVFDADRALREAESALLRKKGSAELTGLLERETQLA
                     LRLEDRREAAMRLERLADLCAQVPGPQMTDALIAILNDSEPRVRVAAGEALRDLGYER
                     YAEVARGIERALDGGADGLAMSELPWVLAEIAEPSALPLLRRFLDHRSADVVAAAIEA
                     LAQLQDPDAIPDLERFLGDARVVVIEDFEDETKTTLGELAADALEMLPVESEG"
     gene            complement(10677..>11829)
                     /locus_tag="VFG22_02935"
     CDS             complement(10677..>11829)
                     /locus_tag="VFG22_02935"
                     /inference="COORDINATES: protein motif:HMM:NF021466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008195 - phosphatidate phosphatase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatase domain-containing protein"
                     /protein_id="HET6415226.1"
                     /translation="ALVDDHRAQRHVGDHSSQLALHLVPVGCYGQAVAPDLSQRRNEI
                     VYRWDLDKTYLRTEFDTLRDLVRTAFEPASRKRAYPGASTLLREIRATDPAAIFILSG
                     SPEQMRSVLEAKLRLDGIRWDGFTLKPSLRNLVRGKFRFLRDQLSYKLTALLRSRTNV
                     SPETDEILFGDDAEGDAFIYSLYADIAAGRVTQELLMRIAELAEVYPEDIPELVRIAA
                     RVPRSDTVRRIFIHLERVSSTAGFGDFGRRVCPFYNYFQPALVLLEDGALDAQAVLRV
                     GADLVVAHTFNPEVLVASFDDLHRRGYISKKVVDRITELSHTIEPSTFGQASGPLQAL
                     VRAVAAARDELPDEVQVDAVKEDYLTLFSKDRARAKAAKRRALWTRDSP"
BASE COUNT         2302 a         3637 c         3664 g         2226 t
ORIGIN      
        1 gtcggaggac gaaaaaacca agggcgacag tggcgacctt cagttcttcg aagcggaagg
       61 cgaaggtccg ccgcccgcct ccattcgtaa agcggcattc tcgatcagca aagtcggcgc
      121 tgtatatccg gagctggtcg aagatggcga cggctaccac atcgtcatgc tgactggaaa
      181 acgggccgcg ctgactcgga catacgagca atcgaagcgc gccatacggc acaagctcac
      241 tcgtcaacgc aaggatgccg caatggaggc gttgacggag cgacttcggc agatgatcga
      301 ggtcgatgtc gactacagcg tcctccagga tatcaaggtc catgtggacg acctgcctcc
      361 aaagccatga ggggcgttct cgccgtcgcc gcgctcgtcc tggcgctcgc cgccggagcc
      421 gctgatgctg acgtcgtcga acgcgtggta gcaaccgtcg acaaccaagc gatcttcctc
      481 agcgacctgc gcacccgtgc tgttccgttc ctgccgcagg tcgccgatgc aacgacggag
      541 accgagcgga aggctcgcct caaggagctc tatgaagagc tgctgaacta cttgatcgac
      601 gagcagctca tccgccagct cgcgaccagc agcggcgttc gaatcaccga tgcggacgtg
      661 agtaatgcga tcgagaacct tcgcctgcag aacaacatga cggaacagca gttccagcaa
      721 gctctcgtgc aacaggggtt caccgaggcg cagtaccgtc aggacctaaa gcggcagctc
      781 ttacggctca aagtcctcaa cgagcgggtg cgttcccgcg tcaacgtgac cgaggaagaa
      841 gtccgtgcac gctacgagca gcgtgcgcgg ggcgaaggca acgcgatccg gtttcatgtg
      901 tcgcacgtcg tggtaccggt cgcagacggc gcgtcggcca tccaacttgc ggcgaaacgt
      961 caggaagccg cctcgctgcg cgagacgctc acgcccgaga atttcgaggc acgtgccgaa
     1021 gcactgggcg gaggcgatct gggctggctg tcggaaggcg acctgcccga ggcgctcgaa
     1081 gtcgctctcg cgcctctcaa tccgggagac atcagtgacc ccgtccgagg cggagccggc
     1141 tttcacatct tctttctcaa agaccgtcag atcggagacg acttcccgac ctacgacgag
     1201 atgaagcaag agctctatcg cgagatgctg gacgccgcga tgatcaagca ggagaaagtc
     1261 ttcctcgacg aagtgcgccg caaggccgtg atcaatcgca tgctctagct tcccgagctc
     1321 gagaccgtcg agcttggcat cggtttgctg ctaggctgtc ggcgatgagc ggcgagttga
     1381 gaatcgaaaa agacgggtcg attgcgcagt tggtattcga ccacgagaaa cgtcgaaatg
     1441 cgatcactgt cgacatgtgg gaggccatac cgagcgctgt ccgtaccgta caggacgatt
     1501 cggagatccg cgtcgtaatc gttcgtggtg ccgggtcgga ggccttcgtt tccggagccg
     1561 acatctcgga gttcgaaaag actcgagccg gcgcctccgg aagagagtac gacgcgatga
     1621 ccgctcgagc gtttgcggct ttgcaaggcc tcgacaaacc actggttgcg ttgattcatg
     1681 gcttctgcat cgggggcggc gccgccatcg cattgaccgc ggatcttcgc tatgccgccg
     1741 acgacgcttt gttcgcggtt ccgccggctc gcctcggcct tggctatcat accgccggca
     1801 ttcgcgcctt gattcgcacg gtcggttttt cggagaccgc ggatttgctg ttcaccgcgc
     1861 gccgggtacc tgcagatgag gctcgtcaca tcgggctggt caacgaagtg ttcgcgaagg
     1921 acgttctgga tgaacgagtc gaagccattg cgcatcggat cgccgagaac gcaccgctca
     1981 ccattcgaag cgcgaagatc agcttgcagg agctggcaaa gctcgagacg gagcgagaca
     2041 gtcctcgcat cgcggagtcg atcgagcgat gctatcagag cgaagacttc agggaaggcg
     2101 taagggcgtt cttggagaag cgaaagccgc gtttcaccgg caggtagcgc tgctgcaagc
     2161 ggtgacgtag accccggcgg agaggagcaa tcgcatgcta gaaaagcaat ctactggtct
     2221 cttggccttt tggcgcgact acgaacgcaa gacctacgtc aaagccgcag tcttggcgga
     2281 ccgcttggga tacgattcat tctggttgcc cgaggtctgg ggatacgagg tcttttctct
     2341 cctgacggaa atcgccctca agaccaagcg catcaaggtg ggtaccggca tcgtcaacgt
     2401 attcagtcga tcgcctgcgc tcattgccat gcaggcggcg actctcgacg agatcagcgg
     2461 cggacgcttc atccttggaa ttggtacgag cggaaagaat gtcatcgaag gtctgcacgg
     2521 ccgcgacttc gagaagcctc tgacgcagac ccaggacgtg attcgcgtgg tccggaccat
     2581 gctcgacgga cgaccgctca gcgaagccgg tgccaagctt cgccagtaca gaccgttcac
     2641 gctcgatttc gaaccgacgc gcaaacgcat ccccatctac gtcgcggcat tgaagcagaa
     2701 gtcgatcact agcgtcggag aaatggcaga tggctggatg ccgatcttct ggccttacaa
     2761 tcggctggag gacgggcgaa ggtggatcgc ggaaggcgct gcgaaagcgg gtcgtgaccc
     2821 gagcgagatt gcaacggcac ccttcaccac ggtcattccg attcccggca agggtgcgtc
     2881 agcgaaggcg cgcgagctca tctctttcta catcggcggt atgggcgact actacaagga
     2941 gttgctcagc ggttttggat acgccgagga gtgcaacgaa atcgagaacc tctaccgcga
     3001 caaagcgacg cggaaaatgg cgcccgatgc agtgacggac gacatgatcg aggcgcttac
     3061 gatctccggc gatccgcttc actgtcgccg cgagatcgcg agacgccgag agctcggcat
     3121 ggagcaaccg atcatcaacc tgcctccggg aatgccctgg cctggcctag cggcgttcat
     3181 tacagcactg gcgcgtgcgt aacgttgagc gcttgcaggc tcattgcggc accacattgg
     3241 ctcgaaatca cggaaaaaat acgtacgaat gagaacaatt acgtatagat cggtccggcg
     3301 agagcacgta ttaaagaaac ataaggggct aagcaagaca gagatccaaa tcgtgcgctc
     3361 agccacctgc tcggttttcg gactgagcat cgacgcggcc ggtcggaaac gggctgaccg
     3421 gccgcgtctt ttttttaatg ctcgagcagg aagaccgcta cttggatttc ggcgcgtcgc
     3481 catcggtcat cgacgcgatc aattcagcct gcgagttcac tccggctttt cgaaagatcg
     3541 ctccgagctg agtggttacg gtgcgcaagg atgcgttccg ctgctcggcg atttccttga
     3601 ttgtgagacc tttcaacacg gcgtcgatga ttgcgtgctc ggccggtgtc gtatcgatcg
     3661 gataacgcaa tttcgggagc ggaacgctca aaacgagaat gtcctcgccc cctacacgca
     3721 tccggctgac gcggggtccc gcatcggttt cccgctcttc gggcgacgtt tcggcctctg
     3781 cggaatcctc ttcgtctcct gagctgacct cggctgtcac gtgccgaagc taaccgccgc
     3841 gccctgaaag cgtcaagcga ggagtgcggg tatgcccgac ttcgggggct ccgctaaagg
     3901 cccatctgtt tcgcgatgat gaccttcatg atttcgttgg tgcctgcgta gattcgctgc
     3961 acgcgcgcgt ccatgtaagc ccggctgatc gggtactcga gcatataccc gtagccgcca
     4021 tacaactgca ggcaggcatc gacgacgcgt ccctgcattt cgctgtgcca aagcttcgac
     4081 atcgagcact ccttgaccag gtacttgcct gcaacgtgct gcgcgaccac ggcatcgaga
     4141 aacgccctac ccacttcgac ctcagtggcg cattgagcga gggtgaactg ggtattctgg
     4201 aacttggaaa tcgaacgtcc gaacgccttg cgttcttgtg tgtacgcaat ggcgtcttcg
     4261 agaactttct cagcgcttgc ctgcgacatg atcccaacac acagccgctc ctgttggagg
     4321 ttctgcatga gcatcatgaa gccggcgccc tcttgaccga gtaagttgag cgcgggaacc
     4381 cggcactcgt cgaacgcgag ctctgcggta tcctgacttt ccatgcccat cttctcgagc
     4441 cgccggctct tgatgaagcc gggcctgtcc gcctcgacga ggaacaacga gacgcttcgg
     4501 tgtgggtcgt cagagttctc cgtgcgcgcg gcgacgacag ctaggtcgca cgacatgcca
     4561 tttgaaatga acgtcttcgc tccgttgatc acgtactcgt caccatcgcg cttggcggtg
     4621 gtcacgatcc ccgcgagatc ggaaccggtc cccggctcgg tcatggcgag cgcggtgacg
     4681 gcttccccac ttgcacaagc gggcagccac ttcgccttct gctcttggct tccgaacctg
     4741 tagatgtagg gaacgatgat gtcggaatgc agactggcgg cccagcccga ctcgtacgct
     4801 cggcaaagct cttccatcac cacgacggag tgaaggaagt cccctcccgc gccgccgtac
     4861 tcctcttcca tccacgggca gaggaatccg gactcaccgg cttccagcca gatctctcgg
     4921 tcgaccacac cctgcgagcg ccacttcgct tgctcgggga cgacccgggt ctcgacgaac
     4981 tgccggaagt tgcggcgaaa gatctcgtgt tcttcggaaa ataagcttcg ttccatcgtg
     5041 ctccctagac tgaatggcca ttcatttacc cgagctgtcg cggcctggca aggcagactc
     5101 gtgctcgctg ccttgcccaa ggcttgcagc ctctctatgg tcccgcgcga tgcgccgccg
     5161 accggcccgc ttttcattgc tgcttgtctt gttcgccgcc attcccgcca ctgcactggc
     5221 gagcggcgac ggtcacgggt tgagttacga catctggctc gtgctcgccg gcatgatctt
     5281 ggcggcgaag ctaggcggag aggttgcggt ccgattcaat cagcccgccg tgctcgggga
     5341 gctctgcgtc gggatcctgc tcggcaatct ctacctcgtc ggcatcgaaa cgttccacgg
     5401 agcggctgag ctcggcccga tagagtttct cgcagagttc ggcgtggttc ttttgttgtt
     5461 cgaggtcgga ctcgaatcca ccctcaccga gatgcgggcg gtcgctccga cctccgggct
     5521 ggtcgccatc ataggcgtcg tagcacccat gctcctcgga tacggtgtga gcctctggtt
     5581 gctccctgag gcatccttct cgctgcactt gttcatcggc gccacgctct gcgccaccag
     5641 cgtcggcatc acggcacgcg ttttgaaaga cctcgacgcg atgggcctgc ccgcgacccg
     5701 tgtcattctg ggtgccgccg tcatcgatga cgtgctcggg ctgatcgtct tggcggtggt
     5761 cgccagcatc gcagaattcg gcgcagtccc cgggccccta gacctgagca agatcatcgg
     5821 gcttgctgcg ggtttcctga tcggtgcgct tcttctgggc gcctacgtga tgcccggcgt
     5881 cttccggatg gcttcgaagc tccgaaccca ggacgttctc gcagccgtgg cgatcgggct
     5941 ttgcttgctg ctcgccggga tctcgggcgc cgccggcctc gcgccgatcg tgggcgcctt
     6001 tgctgcgggt ctgatcttgg acgaggttca cgtccgaccg tttggccgca agtccgcgca
     6061 cgacctgaac gagatcatcg gcccaatcgt cgccgtcatg gcgccgatct ttttcgtccg
     6121 aaccggaatg atggtggatc tgggcggaat cggcaccgag cctctcatcc tcgcagccgc
     6181 gctcactgcg gttgcaatca tcggcaagct gatttcgggt ctcggcgttc gtggggccac
     6241 agccgacaag ctgacggtcg ggattggcat ggtgcctcgc ggcgaggtcg gcctcatctt
     6301 cgcaaacgtg ggcgcgggca tcacgttcga aggcaagcct ctcatcgccg caggcgtgta
     6361 tgcggccatt gtcctgatgg tgatggcgac cacggtggtg actcctccgt ggctggcaca
     6421 tcgcatccgg caggtatcgc gcaagctcga agatgtcgct tgaagcgacc ctagcaaact
     6481 cgaacgcatc gattcgcgac ctggttgcgt cgctcggcgc gaacgaacgg gtcgaggcag
     6541 cgtcggtacc gcgcggggcg ctcagttggg cgatcgcgaa ggctgctgcg gccacctcac
     6601 agcgccgctt cgtactcgtc acctcggacc tcgaggaagc ctatcgctac gagtcgaacc
     6661 ttcggtttct cctcgccgac gatgacggcg acgttcttct cttcaccgcc gcggacacga
     6721 gcccgcttct cgacgtcgta cctgaccgtc gggccgagat gcagcgtatg gccgtgctcg
     6781 cccagctggc cgaggagcgg ccttggcgcg tcctcatcgt gccggctacg gcgctcctcc
     6841 ggcgggtgcc ccctgccgac catgtgaaat ctggactgct cgggatcgaa ttcggcgatc
     6901 ggatcgaacg cgatgagctc gttcggcggc tcactgagct gggatacctg cgcgtcccac
     6961 tcgtcgagga tcgtggaacc ttctcggcac ggggcgcact catcgacgtc tacggacccg
     7021 acgcgtcgct gccagttcga atcgagttgg acgacgacct gatcgtgagg attcgccgct
     7081 tcgatccgga agaccagaag agcgccgacg agatcgaatc cacgcagctc gccgccgctc
     7141 gagaggtacc cgacaccgcg aaagaaatct cccgggcgaa gacggcggtt cgcgagctct
     7201 gcgacgagat gaacatgccg acattgcgag ctcgcgagtt gatcgacgag ctcgaccgcg
     7261 gctcgggcgc cctgatctcc aatgcgctcc taccggccta cttcgaggcg ctcgacaccc
     7321 tgttcgatta cattccgtcc gatgctcggc tcgtattggc cgaccctgca gccattgccg
     7381 aatcggtccg cacggaagag cgccaagcca aggacgaaca cgccgcacga cgcgaccggc
     7441 ctaccttcga gctagagaag tatctcttga gcgaagcgga gcttgcgaac cgactgcgag
     7501 gatacagccc gcttgtcgtc catcggctgg ccgtgcacgg cgccgcagcc gaagacgagg
     7561 ggccgatcgt tgccgccttc acaccgccgg caaacgagct catgcgcgtc gacgcctcgg
     7621 atcaggccga tctcatctcg aagcttcgcg cgcagaaggg acaccgcgaa cggggtctgc
     7681 agccgctcgc cgatcagatc cttcaatgga cggaagaggg attgaaagtt tcgctcatag
     7741 gcagggctca gcatcaggcg gaccgcttga tcgacttgct gcgcagctat ggcgtcgcag
     7801 caaacgtcct cgacggcatt ctcttgggcg agcttcgaga cggctttgtg ctctggagcg
     7861 aaggcatagc ctacgtgact gaggaagaga tcttcgggtc gcgggtccga caacggcgca
     7921 gcaagcgtca atcgaggcgt cagcagcaac gattcctgga agatctccgt gagctgaacc
     7981 caggtgactt cgtggttcac gtcgaccacg gagttgggaa atatctcgga ctccagcaca
     8041 aggcgctgag cctcacggca ctggaccgac ttcacgggcg aacgccacag accgtcgaag
     8101 tgatggtcgt cgagtacgca gggagcgaca aactcttcgt gcccgtcacg cgcctcggag
     8161 tgatccagaa gttcaaaggg ggtgaggggc atcagccgaa gctcgaccga ctcggaggca
     8221 cgacgttcgc agcgaagaaa gggaaggtcc agaaggctgt gcagcagatg gccgaggagc
     8281 ttctcaagct ctacgccgag cgctcggccg cgcgccggga gccactcgaa cccgccgaca
     8341 cggcgtacgc cgagttcgag gcaacgttcc ctttcgagga aacgcgtgac caggagaagg
     8401 cgatcaacga tgtcatggcc gatctcgagg agccgcaacc aaccgaccgg ctagtctgcg
     8461 gagacgtcgg cttcggcaag acggaagtcg cgctgcgcgc agcgtttcgt gtcgccatgg
     8521 gcggcggaca ggtcgccgtc ctctgtccga cgaccgtcct ggcccagcag cactaccgga
     8581 ccttctctgc gcgcttggat gggtatcccc ttcgtgtcga agtgctctcc cgattcgtct
     8641 cgcgaaccaa gcaaatcgag gtactcggcg cgctcaagga cggaaaggtc gacgtcgtca
     8701 tcggaaccca caggcttctc tcgaaggacg tgcactttgc caagctcgga ctcttggtcg
     8761 tcgacgagga acagcggttc ggtgtgaccc acaaggaacg catcaagaag cttcggacca
     8821 atgtcgacgt gattaccctc agcgcgacgc caattccgcg caccctgcaa ctggctgtgg
     8881 gcgggatgcg gaacctatcg ttgatcacga ctgcacccca ggaccgacgg gccgtgcgca
     8941 cgttcgtctg ccgctgggac gaccatctga tcaaagaggc cgtggagcgg gagctcagcc
     9001 gtggtggaca agtcttcttc gtctacaatc gcatcgaagg cctctatgag cgcgctcaac
     9061 ggctgcaaga gctgctgccg aatgcgagaa ttgcggtcgc ccacggccaa atgaagccag
     9121 cgctgctcga caagacgatg actgatttcg tcgagggtgc atatgacgtg ctctgttcga
     9181 ccgccatcgt cgagagcggg ctggatattc cgcgcgcgaa cacgattctc gtcgaccgag
     9241 cagacacact cggtctcgcc cagctctatc agctgcgggg acgcgtcgga cgatcggatc
     9301 aacgcgcgta ttgctacttg atcaccccgc cgccgaacca gatgtccgag gagtcgcgcg
     9361 ttcgcatgga agcgctcgag agattttcgg gactgggagc tgggttccga gtcgcaacgc
     9421 tggacatgga gctccgaggg gctggaaatc ttctcggctc cgagcagagc ggaaacgccg
     9481 agctcgttgg attcgatatg ttcgtgcaga tgctcgacca ggcagtatcg gagctcaagg
     9541 gtgaccatgt tcaacaggag gtcgaccccg agctttccat cgagctagag cattacctac
     9601 cagaggacta catcgatgac atcgggctgc ggctctcgct ctatcgtcgc ttcgccaccg
     9661 cgatcgacga acaggccatc gaagatctgg ccgaagagat ggaacaacgt ttcggagcgc
     9721 cccccaacca ggcgcgcgat ttcgttcgtg tgatgtccct caagccgccg ctccgggagc
     9781 tccgcgcgct cgggtgcgaa ggcgagctga agcgcgtcac gttgcacctg cgcgaggata
     9841 cgccgctcga cccagcgaag ttgatgcccc tggtcgcgac ccccggtgca ggctggagcc
     9901 tttcgcctga catgaagctc actcggcgct atcgagacga tgaatccggg gacgccgtag
     9961 atcgtgtaag gtccctgctt cgagagctcg agcccatgcg ctcggcggcg gcacaacagc
    10021 aaaaggacgg ttcagacgat gtcacttccc gatgagctcc agcgcgtatt cgatgccgac
    10081 cgcgcacttc gagaggccga atccgcattg cttcgaaaga aaggctccgc agagctgacg
    10141 ggcctgctcg aacgcgaaac tcaacttgca cttcggctcg aggatcgtcg agaagccgcg
    10201 atgcgcctcg agcggctcgc cgatctctgc gcacaagtcc ccgggccgca gatgaccgac
    10261 gccttgatcg cgattctgaa tgattccgag ccgcgcgtcc gcgtggccgc aggtgaagcg
    10321 ctccgggacc tgggctacga gagatacgca gaggtcgcgc gaggcatcga acgggctctc
    10381 gacggcgggg ccgatgggct cgcgatgtcc gagctcccct gggtgctcgc cgaaatcgcg
    10441 gagccgagtg cgctgccgct cctccggcgc tttctggatc atcggagcgc cgacgtggtc
    10501 gcagccgcca tcgaggcact ggcgcagctc caggatccgg atgcgattcc cgatctcgag
    10561 cgattcttgg gtgacgcacg agtggtggtc atcgaggatt tcgaagacga aaccaagacc
    10621 actctcggcg agctggcggc cgacgctctc gagatgttgc ccgttgaaag cgaaggctaa
    10681 gggctgtcgc gtgtccaaag cgcgcgtcgc ttcgctgcct tggcgcgagc ccggtccttg
    10741 gaaaagagcg tgaggtagtc ttccttcacc gcatcaacct ggacctcatc gggaagctca
    10801 tcgcgtgcag cagccaccgc gcggacgaga gcttgcaagg gtccggatgc ttgtccaaac
    10861 gtcgaaggct cgatcgtatg ggagagctcg gtgattcgat cgaccacctt cttgctgatg
    10921 tagcctcgcc ggtgcaggtc atcgaaactg gcgactagaa cctcgggatt gaacgtgtgg
    10981 gcgacgacca ggtcggcacc tacacgcaga accgcctgtg catcgagcgc gccgtcctcg
    11041 agcaggacca gagcaggctg gaagtagttg tagaacggac acactctccg cccgaaatcg
    11101 ccgaaacccg ctgtcgagga aacgcgctcg agatggatga agattcgacg caccgtatcg
    11161 gagcgaggaa cgcgagcggc gatccggacc agctctggga tgtcctcggg atagacctcc
    11221 gccagctcgg caatcctcat cagcagctcc tgagtcaccc gtcctgccgc gatgtcggcg
    11281 tagagcgagt aaatgaaggc gtcaccttcc gcatcgtcgc cgaagagaat ctcgtcggtt
    11341 tcgggggaca cgttcgtcct cgaacggagc agcgcggtca gcttgtagct gagctgatca
    11401 cgaaggaatc ggaacttgcc ccgcacgagg tttcgcaggc tgggcttgag cgtgaacccg
    11461 tcccaacgga tgccgtccag cctcagcttg gcctcgagca cactcctcat ttgttccggg
    11521 ctcccggaga gaatgaagat agcagcaggg tcggtggcgc gaatctcccg aaggagcgtg
    11581 ctcgcgcccg ggtaggcgcg cttacgtgag gcgggctcga atgcggtgcg caccaggtcg
    11641 cggagcgtat cgaactcggt tcgaaggtag gtcttatcga ggtcccaccg atagacgatc
    11701 tcgtttcgcc gctgactcag atccggtgcc acggcctgac catagcagcc aactggcacg
    11761 agatgcagag ccagttggct gctatggtcg ccgacatggc gttgagcacg gtggtcatcg
    11821 acgagcgca
//
LOCUS       DASRZF010000065         6225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3831239, whole genome shotgun sequence.
ACCESSION   DASRZF010000065 DASRZF010000000
VERSION     DASRZF010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 6225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6225
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3831239"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            494..>6225
                     /locus_tag="VFG22_02940"
     CDS             494..>6225
                     /locus_tag="VFG22_02940"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6415227.1"
                     /translation="MTSKTTASGPIAIIGLGCRYPDAKSPRELWENVLSRRQSFRAIP
                     DVRLPLSEYGDSDKSAPDATYAKRAAVIDGFEFDWARRRIPKQVFEQTDITHWLALDV
                     ALQALADSGYDPKELPRDRTGVIIGNTLTGEQQRAHLLRGRWPFVRKTIRAAGIKKGL
                     DAATLEELVAETEHIYKSFFPAPNEDTLAGALANTIAGRICNYMDLRGGGYTVDGACS
                     SSLLSVATASNALCAGDIDVALAGGVDVSLDAFELVGFAKVGALSDSDMKVYDRAGKG
                     FLPGEGCGFIVMKRLEDAKRDGDYIYAVLNGWGISSDGKGGITAPTVDGQALALRRAY
                     DRAGYSAHSLDFVEGHGTGTAVGDPVELQAISNTIDSFGEAEPHRCGVTSFKSLVGHT
                     KAAAGIGGLLKAIAGVNRRVIPPTAGCDEPHEIFSSHAKNVYPVRTGAMRDPSSTLTA
                     GVSAMGFGGINSHVTVTSGAAPSIDLEPSLDERALLASKQETELFVLSAESLTSLGKK
                     ATELLDVVAGMSIGEMPDLAVELAQRLVPAATTRAAIVASSPEDLHKRLGTLLSAIEK
                     ADSDADYSFSSPDRRVMLSVGATSPRVGFLFPGQGSQRVNMARVLVERFGWARELVAN
                     ADRWLDEVGVAPVSGFIFRETDPDLSGEKQKEWMRALTQTEVAQPAICLASLLWLRFM
                     KQLGLEPSAVAGHSLGELTAFHAAGALDEKSTLQLAGLRGKLMGEVPVEGSMASLVCD
                     PDEARALLEQIDGYVVIANINSPTQTVISGDSAAVAAAVERASNQGIRARELPVSAAF
                     HSAHFKDTVTALRQTDVLRGTAASKDTTLISTMTGAVLEGDVQLADHFGEQVVRPVNF
                     RAAADLATASCDVLIEVGPGAVLSGLSNETAGPGGTVSLPVESKAGRDRDLNTALAHL
                     FTRGAEIDLLKLWDQRLIRRFKSAAELSFIVNPCEKDLIDTESVKKLATKAPAPARPA
                     RTPEPVTGIRPIGWKATQDEHETVRPSRPASASPYVDTRSVLLDAIHKQTGFPADTLT
                     DDLRMIDDLHMDSIKTVQVVVDVAVAIGYAGDFDPADIANSTLGEIVQELEKRIATAK
                     EGRSGSLSPTRAAEIAKNYPGWTRNFVMRWQDATLPDAPENFWKRRSLCVLDDGSEAA
                     TALWEHTRKLGADVQAVPIAAASEINADLIVAVLSDEAMGSSTQERFANVTELLASIA
                     GGLPLSTSTESPQTIVFVQRRSAQSDYLDWGYDGFVAALHLERKRLRFRALTVDESVD
                     GDLLCALIARETETDVRYDAARYFGRTRRIARPQILAPTALTRRTKQLDASDVVLVTG
                     GARGITAECALALARETGAHMVLAGSSPSPEDAPESPNSAEISKTLERFAEEGLSAEY
                     RQANLTDRDEVSHLVAAARRKTGRISAVIHGAAINRPRRANKVSADEARSEISPKLIG
                     ALNLFDALRNDPPAIFCAFTSVIGVTGMPNNAWYAFANQALDRSLGLFASSQPNTDAI
                     SMAFSVWEEVGMGANLGSIEGLSSLGTDSIPLQEGVRRFLELFTQDAGDRQVIVTGRL
                     GGIDTWRPEFPVLPKGRRFLENVRFFQPGVELVARTHLSLERDAYLTDHEYKGVCLFP
                     TVFGVEAMAQAVAYVLGRSELRSVTISDLDLSRPLPVHSERGLEIEVYARVSEDEPGS
                     LNVKAGIRCEQNGFSVDHFSGTFSLAVQASRRTDPSVPKTTTTLSLDPQRDLYGPILF
                     QGSRFQRIKRLHALDAVTCNFESEERESADFVLGDPFARDALLQSLQLCALPDQCLPV
                     HIGRWQIAEASDRSAGTRFNQSTITSHTDEAYVGNIISSDESGVVLEELSNYRAQILE
                     RRDDWPNARQLASGVAKSLSLESDSDWSAGNSFYTIEEGGPRGQVIFAFRFPLTFRNS
                     ANPLRTVYFS"
BASE COUNT         1163 a         2106 c         1875 g         1081 t
ORIGIN      
        1 tccgacgacg aatgcccaac gggtggaata tgtcgcgaag ttggcccgct gcagaaccga
       61 tgcacatacc tatgcggtct tccggcgcag tgccctgccg atccacccgc caacacctgc
      121 ggctcctccg gccccagcgg cgacgattac tgcggcggct aaaaagcgct gcgatccgtg
      181 cttgcgatct cacgcgggaa acggcaatct cgccaaaccc cgttactgct ccgaaggagc
      241 cggacctgcg atccccaccc tagaccggat gggtgtgctt cgcggggagg tctcatttgt
      301 gacatctttc acaattcgac aatggaagcc tccatctgca ctcctgcaag gtactgaaac
      361 catttattta ttttgaaaac atgaataaaa gtcactctcg ctgagccgct gttcagcgga
      421 tgcgagagcc ggcctcggta tactcgctct cccccgtttg tcttacttgg acccttaacc
      481 cgttgtccca attatgactt cgaaaaccac ggccagcggc cccatcgcga tcatcggtct
      541 cggctgccga tacccggacg cgaagagccc tcgagagctc tgggagaatg tcctctcccg
      601 gcgccagagc ttccgcgcaa tcccggatgt gcgtctgccg ctctcggaat acggcgattc
      661 cgacaagagc gcccccgacg cgacctacgc gaagcgcgcc gcggtgatcg acggcttcga
      721 gttcgactgg gcccgtcgcc gaatccccaa gcaggtgttc gagcagaccg acatcaccca
      781 ttggctcgcg ctcgacgtgg ccctgcaggc cttggccgat tccggatacg atccgaaaga
      841 gcttccgcgc gatcgaaccg gcgtcatcat cggcaacacg ctcaccggcg agcaacaacg
      901 cgcgcatctt ctccgcggcc gttggccgtt cgttcgtaag acgatccgcg ctgccggcat
      961 caagaaaggc ctcgacgcgg ccacgctcga agagctggtc gcagagaccg agcatatcta
     1021 caagtcgttc ttcccggcgc cgaacgagga cacgctggca ggcgccctcg caaacacgat
     1081 cgcaggccgc atctgtaact acatggatct gcgcggcggc ggctacaccg tcgacggggc
     1141 ttgttcttcg tcgctgctct ccgtcgcgac ggcgtccaac gcgctttgcg ccggagacat
     1201 cgacgtcgcc cttgcagggg gcgtggacgt gagcctcgac gcgttcgagc tcgtcggctt
     1261 cgccaaggtc ggcgcgctct ccgattcgga catgaaagtc tacgaccgcg cgggcaaggg
     1321 tttccttccg ggcgaaggct gcggcttcat cgtgatgaag cgactcgagg acgcgaagcg
     1381 cgatggcgac tacatctacg cggtcctgaa cggctggggc atttcctccg acggcaaggg
     1441 cggcatcacg gcgcccaccg tggacggcca agccctcgcg cttcgtcgcg cctacgaccg
     1501 cgccggctac tccgcccaca gcctggactt cgtcgaaggt cacggcactg gaaccgcggt
     1561 tggcgacccg gtggagcttc aggcgatttc gaacaccatc gactccttcg gtgaagcgga
     1621 gccgcatcgg tgcggcgtga cctcgttcaa gtcgctcgta gggcacacca aggccgcggc
     1681 cggtatcggt ggcttgctca aggcgatcgc cggcgtcaat cgacgtgtga ttccaccgac
     1741 ggcgggatgc gacgagccac acgagatctt cagcagtcac gcaaagaacg tctatccggt
     1801 tcgcaccggc gcgatgcgcg atccctcgag caccctcacc gcgggcgttt ccgcgatggg
     1861 cttcggtggc atcaacagcc acgtcacggt cacatccggc gctgcgccgt ccatcgacct
     1921 cgagccttcg ctcgacgagc gcgcgctcct ggcctcgaag caggagaccg agctcttcgt
     1981 gctctcggcg gagagcctga cctcgctcgg gaagaaagcc acggagctct tggacgtcgt
     2041 cgccggcatg agcatcggcg aaatgcccga cctcgccgta gagctcgcgc aacggctcgt
     2101 ccctgcagcg acgacgcgcg ccgccatcgt ggcgagcagc cccgaggatc ttcacaagcg
     2161 cctcggcacc cttctgagcg caatcgaaaa agccgactct gacgcggact acagcttcag
     2221 ctctccagat cgacgcgtga tgctcagcgt gggcgcgacg agcccgcgcg tcgggttctt
     2281 gttccccggc caaggctcgc agcgcgtcaa catggcgcgt gtcctcgtgg agcgcttcgg
     2341 ctgggcgcgg gagctggtcg cgaatgccga tcgctggctc gacgaggtcg gcgtcgcgcc
     2401 ggtgagcgga ttcatcttcc gcgaaaccga cccggacctc agcggcgaaa agcaaaaaga
     2461 atggatgcgc gccctcacgc agaccgaggt cgcgcagccg gcgatttgcc tcgccagcct
     2521 gctctggctg cggttcatga agcagcttgg cctcgaacct tcggcggtcg ccggacacag
     2581 cctgggcgag ctcaccgcgt ttcacgccgc aggcgcgctc gatgagaagt cgacgctgca
     2641 gcttgccggc ctgcgcggca agttgatggg tgaggtcccg gtcgagggtt cgatggcgag
     2701 cctagtctgc gatcccgacg aagcgcgcgc cctgctcgag cagatcgatg gctacgtggt
     2761 gatcgcgaac atcaacagcc cgacgcagac cgtgatctca ggggatagtg cagccgtcgc
     2821 tgcggccgtc gagcgggcct cgaaccaggg tatccgagca cgcgagttgc cggtgtccgc
     2881 ggcgttccac tcggcccact tcaaggacac ggtcaccgcg cttcgccaga ccgacgtgct
     2941 tcgtggcacg gcggcttcga aagacaccac tctcatcagc acgatgaccg gcgctgtgct
     3001 cgaaggcgac gtccagctcg cggaccactt cggtgagcag gtcgtccgtc cggtgaactt
     3061 ccgggcggca gcggacttgg ccacggcgag ttgcgacgtc ttgatcgaag tcggtccggg
     3121 cgcagtcctc tcaggcttga gcaatgaaac cgcaggcccg gggggcaccg tctccctgcc
     3181 cgtcgagagc aaggccggcc gcgatcgcga cctcaacacc gcgctcgcgc accttttcac
     3241 gcggggcgcg gagatcgacc tcctgaagct ctgggaccag cgcttgatcc gaaggttcaa
     3301 gtccgccgcc gagctctcgt tcatcgtcaa cccctgcgaa aaggatctca tcgacaccga
     3361 atcggtgaag aagctcgcga cgaaggcccc tgccccggcg cgcccggcac gcacaccaga
     3421 accagtgacc ggcatccgac ctatcggttg gaaggcgacg caagacgagc acgaaaccgt
     3481 gcggccgagc cgccccgcga gcgcttctcc gtacgtcgat acccgttcag tccttctcga
     3541 cgccattcac aagcagaccg ggttcccggc cgacacgctc accgatgacc ttcggatgat
     3601 cgacgacctg cacatggact cgatcaagac ggtccaggtc gtggtcgacg tcgcggtcgc
     3661 cattggctac gccggcgact tcgaccctgc cgacatcgcc aactcgaccc tcggcgagat
     3721 cgtccaggag ctcgagaagc gcatcgcgac tgccaaggaa ggccgctcgg gcagcctttc
     3781 gccgacacgt gccgccgaga ttgcgaagaa ctatccgggc tggacccgca acttcgtgat
     3841 gcgatggcag gacgccacat tgccggatgc tccggagaat ttctggaagc gccgttcgct
     3901 ctgcgtactc gacgacggct ccgaagccgc aacggcgctt tgggagcata cccgcaagct
     3961 aggtgcggac gtccaggccg tgccgatcgc agccgcatcc gagatcaacg cggatctgat
     4021 cgtcgccgtt ctctccgatg aggcaatggg gtccagcacg caggagcgct tcgcgaacgt
     4081 caccgagctc ctcgcctcca tcgcaggcgg cctcccactc tcgaccagca cggaatctcc
     4141 tcagaccatc gtgttcgtcc aacggcgctc cgcacaaagt gactacctcg actggggcta
     4201 cgacggcttc gtggccgcgc ttcatttgga acgcaagcga cttcgcttcc gagcactcac
     4261 cgtggatgag tcggtcgacg gcgatctgct ctgcgccttg atcgctcgcg agaccgaaac
     4321 cgacgtgcgc tacgacgccg cccgctactt cggcaggacg cggcgcatcg cgcgccctca
     4381 gatcctggca ccgaccgcgc tcacgcggcg cacgaaacaa ctcgacgcca gcgacgtggt
     4441 gctcgtgacc ggcggcgcgc gcggcatcac cgccgaatgc gccctcgcgc tggctcgtga
     4501 aaccggggcc cacatggtcc tcgccgggag ctcaccttct ccagaggatg ctcccgagtc
     4561 ccccaacagc gcggagatct ccaagaccct ggaacgcttc gcggaggagg ggctcagcgc
     4621 cgagtacagg caagccaacc tcacagatcg cgatgaagtc tcgcacctgg tggccgctgc
     4681 gcgccgcaag acgggacgca tcagcgcagt cattcacggt gctgcgatca accgcccccg
     4741 gcgcgcgaac aaagtcagcg cggacgaggc gcgaagcgag atcagcccga agctcatcgg
     4801 cgcgctcaac ctcttcgacg cgctcagaaa cgacccacca gccatcttct gcgcgttcac
     4861 ctcggtcatc ggcgtgaccg gcatgccgaa caacgcctgg tacgcgtttg ccaatcaggc
     4921 cctcgaccgc tctctcggcc tctttgcaag ctcccagccg aacaccgacg ccatctcgat
     4981 ggcctttagt gtatgggaag aggtaggcat gggcgcgaat ctcggaagca tcgaaggcct
     5041 ctcgagcctc ggcaccgatt cgattccact ccaagaaggc gtgcgccgct tcctcgagct
     5101 cttcacgcaa gacgcaggcg accgtcaggt catcgtcacc gggcgcctcg gcggtatcga
     5161 cacgtggcgg ccggaattcc ccgtgctgcc gaagggccgc cgtttcttgg aaaacgtccg
     5221 tttcttccaa ccgggtgtcg agctcgtcgc acgaacccac ctcagcttgg agcgagacgc
     5281 ctatctcacc gatcacgaat acaagggcgt gtgcctcttc ccgaccgtat tcggcgtgga
     5341 ggcgatggcg caagccgttg catacgttct tggacgctcg gagctccgaa gcgtgacgat
     5401 cagtgacctg gacttgtcgc gccccctccc cgttcactct gaacgcggac tcgaaatcga
     5461 ggtgtacgcg cgtgtctcgg aagatgagcc cggctcgctc aacgtgaagg ctggcattcg
     5521 ctgcgagcaa aacggcttct cggtcgacca tttttcggga acgttctcgc tcgccgtgca
     5581 ggcgagccgt cggaccgacc cctcggtgcc gaagacgacg acaaccctat ccctcgaccc
     5641 gcagcgagat ctctacggcc cgattctgtt ccagggatcc cgattccagc gcatcaagcg
     5701 cctacacgct ttggacgcgg tgacctgcaa cttcgaaagc gaggaacgtg agagcgctga
     5761 tttcgtgctc ggggatccgt tcgcgcgcga cgccttactt cagagcttgc agctctgcgc
     5821 gttgccggat cagtgtctgc cggtccacat cggtcgatgg caaattgcgg aagcttcaga
     5881 tcgaagcgcc ggtacccgat tcaatcagtc gaccattacc agccacacgg atgaagcgta
     5941 cgtcggaaac atcatttcga gcgacgaatc gggtgtcgtc ctggaagagc tatcgaacta
     6001 ccgcgcacag attctggaac gtcgggatga ttggcccaat gctaggcagt tggcatcggg
     6061 ggtggccaag agcctgtcct tggaatcaga ttcggattgg agtgctggga acagcttcta
     6121 caccatcgaa gagggaggac ccagagggca agtcatcttt gcatttcgct tccctctgac
     6181 gttccgcaac tctgcgaacc cgctgcggac cgtctatttc tccaa
//
LOCUS       DASRZF010000066         2061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1771745, whole genome shotgun sequence.
ACCESSION   DASRZF010000066 DASRZF010000000
VERSION     DASRZF010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1771745"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VFG22_02945"
     CDS             <1..394
                     /locus_tag="VFG22_02945"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HET6415228.1"
                     /translation="GTPSDLLQALSTGVDLFDCVLPTRNARNGQALTWGGRVNLKQAR
                     HREDESPLDEHCDCSVCAGYSRAYLRHLFKADEMLGPRLLSLHNLHFYAALMRKAREM
                     IRLGELGSWVASTLSEMKERDEVGDPDT"
     gene            497..2047
                     /gene="purH"
                     /locus_tag="VFG22_02950"
     CDS             497..2047
                     /gene="purH"
                     /locus_tag="VFG22_02950"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HET6415229.1"
                     /translation="MTEIKRALISVSDKTGVVEFAKGLSDAGVEILSTGGTARALRDG
                     GVSVIDVSEYTQSPEIMDGRVKTLHPRVHGGILMRESSEDRDALGAIGGKPIDLVCVN
                     LYPFEQTVARGASHDETIENIDIGGPSMVRSAAKNQARVTVVTDPGDYEEVLRAVKSG
                     DGPNASLRARLAAKAFAQTAAYDGAISAYLTSQGTDSKFPGALTLSFKKAYDVRYGEN
                     PHQQGAFYIERNAPAGSLALAQSLGAGGKELSFNNLVDVDATIEAVREFEGPAAVVVK
                     HTNPCGVATADTLEKAYRIAREADAQSAFGGIVALNREVDDATAEAVAETFIECIIAP
                     SFAPAALERLQKKKNLRILATGQWLPADYAALQFKRVGGGLVVQDRDATGPSEVRAGK
                     VVTKRQPTDDEMRALDFAWRVCKHVKSNAIIFAKPDRTVGVGAGQMARVTAVGIAAQK
                     AGELSKGAVMASDAFFPFPDGIEAAAKVGISAVVHPGGSKGDDKVIAAADAANMAMVF
                     TGVRHFRH"
BASE COUNT          384 a          644 c          666 g          367 t
ORIGIN      
        1 gggcactccg agtgatctgc tgcaggcgct ttcgaccggt gtcgatcttt tcgactgcgt
       61 ccttccgacg cgcaacgcgc gtaacggtca ggctctgacc tggggtggtc gcgtcaatct
      121 caagcaggcc aggcaccgcg aagacgagag cccgctcgac gagcactgcg attgctcggt
      181 ctgcgcgggc tattcacgcg cctatttgcg gcaccttttc aaggctgatg aaatgctcgg
      241 gccgaggctg ctctcgcttc acaacctgca cttctatgct gcgctcatgc gcaaagcgcg
      301 cgaaatgatt cgactcggcg agcttggttc ctgggtcgcg tcgaccctga gcgagatgaa
      361 agagcgggac gaagtcggcg atccggacac ctgaatcaga gggcggcggc ccgggtgcgg
      421 ctcatcgatt gacgactttc tggctgcggg gtatgggccg gacttccgca tcggcttcgc
      481 gggagaaggg tgaggcatga cagagatcaa gcgcgcactg atatcggtct ccgacaagac
      541 cggcgtcgtc gagttcgcca aagggctttc ggacgctggc gtcgagattc tttccaccgg
      601 cggtacggct cgggcgctcc gagatggcgg tgtttccgtc atcgacgttt cggagtacac
      661 ccaatcgccg gagatcatgg acggccgcgt gaagacgctt cacccacgcg tccatggcgg
      721 gattctcatg cgggagagca gcgaggaccg tgacgcgctc ggcgcaatcg gcggaaaacc
      781 catcgatttg gtctgcgtga atctctatcc cttcgaacag acggtggctc ggggcgcctc
      841 ccacgacgag acgatcgaga acatcgacat tggcggaccc tcgatggttc gcagtgctgc
      901 gaagaaccaa gcgcgcgtca ccgtcgtcac cgatccgggt gactacgaag aagtcctccg
      961 tgcggtgaag tcaggtgacg gaccgaacgc aagcctgcgc gcacgcttgg ccgccaaggc
     1021 ttttgctcag accgccgctt acgacggcgc catctccgcc tacctcacct cccaggggac
     1081 cgactccaag ttccccggcg cgctgacctt gagcttcaag aaggcctatg acgtgcgcta
     1141 tggcgagaac ccgcatcagc agggtgcttt ctacatcgaa cggaacgcgc ccgcggggag
     1201 cctggcgctg gcccagtcgc tcggagctgg tggcaaggag ctttccttca acaatctcgt
     1261 cgacgtcgat gccaccatcg aagcggttcg cgagttcgaa ggccctgcag cggtcgtcgt
     1321 aaaacacacc aatccttgcg gcgtagcgac tgcggacacg ctcgagaaag cgtaccggat
     1381 cgctcgcgag gcggacgcgc agagcgcctt cggcggaatc gttgccctca atcgagaggt
     1441 cgacgatgcg acggcggagg cagtcgccga gaccttcatc gagtgcatca tcgctccgag
     1501 cttcgcgccg gccgctctcg agcggctcca aaaaaagaag aacctccgga ttctcgcgac
     1561 cgggcaatgg cttccagcgg actacgcggc gctgcagttc aagcgcgtcg gcggcggtct
     1621 ggtcgtgcag gatcgcgatg cgaccggccc gagcgaggtt cgcgccggca aagtcgtgac
     1681 caagcgtcag ccgaccgacg atgagatgcg cgcgctcgat ttcgcctggc gcgtttgcaa
     1741 gcacgtgaaa tcgaacgcga tcatcttcgc caagcccgac cgcaccgtgg gtgtcggagc
     1801 cgggcagatg gcgcgagtca ccgccgtcgg aattgcggcg cagaaggcgg gtgagctatc
     1861 gaagggtgcc gtcatggcct cggatgcctt cttcccgttc cctgatggca tcgaagccgc
     1921 agccaaggtg ggcatcagcg cggtcgtcca tccaggtggc tccaagggcg acgacaaggt
     1981 catcgccgct gccgatgcag ccaacatggc gatggtcttc accggcgttc gccacttccg
     2041 gcactgagga tgcgcgttct c
//
LOCUS       DASRZF010000067        19461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3146007, whole genome shotgun sequence.
ACCESSION   DASRZF010000067 DASRZF010000000
VERSION     DASRZF010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 19461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19461
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3146007"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /gene="hisE"
                     /locus_tag="VFG22_02955"
     CDS             <1..285
                     /gene="hisE"
                     /locus_tag="VFG22_02955"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HET6415230.1"
                     /translation="RTLEARKASDAGKSYTKALLDAGPTKIDAKLREEAAELGQALIG
                     ESDERVASEAGDVIYHLLVGLVSRGVSLRELFSELSKRFGQSGHEEKAKR"
     gene            complement(331..819)
                     /locus_tag="VFG22_02960"
     CDS             complement(331..819)
                     /locus_tag="VFG22_02960"
                     /inference="COORDINATES: protein motif:HMM:NF044704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HET6415231.1"
                     /translation="MSFSVGDKAVHPAHGVGEITQIEELLIGSDRIDFYILKLVNGKS
                     GTIRVALNAVDRVGLRQVMSRSAAKTVLEELKKDEMAVTSQPWNRRYREYTEMLKSGS
                     PIKVAKVLRDLSKVRADKGELSYGEKKLMEQARKLLITELALARRVREETIERDIDEI
                     LH"
     gene            complement(1057..2157)
                     /gene="recA"
                     /locus_tag="VFG22_02965"
     CDS             complement(1057..2157)
                     /gene="recA"
                     /locus_tag="VFG22_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HET6415232.1"
                     /translation="MADSNREKALNLALGTIEKAYGKGAIMRLGDESAKIQVPVISSG
                     AISLDLALGVGGYPRGRIIEIYGPESSGKTTLTLHAIAECQKKGGIAAFIDAEHALDP
                     NYARKLGVNVDELLVSQPDNGEQALEIADTLVRSGAVDMIVIDSVAALVPRAEIEGEM
                     GDSHVGLQARLMSQALRKLTGNLQKSHTSLFFINQIRMKIGVMFGSPETTSGGNALKF
                     YASQRLDIRRIGTIKVGDAAVGNRCRVKVVKNKLAPPFRLCEFDILFGHGISRTGDVL
                     DLGVEAGIINKSGAWYSYGDERVGQGRDNARTFLEEHPDVLAAVERKLLQQHGLIEED
                     TPAAPKAEAQTETKAETKAAPAAAASKRARPN"
     gene            complement(2351..3610)
                     /locus_tag="VFG22_02970"
     CDS             complement(2351..3610)
                     /locus_tag="VFG22_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HET6415233.1"
                     /translation="MTAAVLSIGTELTRGELVNSNAAWLGEELTKLGFDVIEHATVDD
                     SLERIVTIVRRFAKEQRVVVATGGLGPTSDDLTTEAAAKAARVGLRRDESVVAGIREK
                     FRAFGRVMPESNAKQGDFPVGATILPNAMGTAPGFTMTLGDCRFFFVPGVPREMKHIF
                     FETIVPLIAPLAEPRTHQEHLRTFGMTESGVAEALRDLEKEHEGLTLGYRAHFPEIEV
                     KVLVRAESAAEAERRSQSIAEQVRERLGDAVFGGRDDSFPEMVGEALRNKGKTLAVAE
                     SCTGGRVGEMLTRVPGASDYLLLDAVAYANSAKVAVLGVSEETLRAHGAVSSETAAAM
                     AEGALRVAGADIAVSITGVAGPSGGTDDKPVGTIWFGLARKGEPTITKLRKLPWGRDR
                     VQRLAAYIALELVRRAALGRDFDLGER"
     gene            3726..5102
                     /locus_tag="VFG22_02975"
     CDS             3726..5102
                     /locus_tag="VFG22_02975"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET6415234.1"
                     /translation="MNRIFKATALGLLVAALTLSAGRPVVAEQSTAALSNLQVSRAKL
                     DNGLRVVMNQDRTVPTVAVSVYYDVGSRNEVKGRSGFAHLFEHMMFQGSANVGKGEHF
                     SLIINRGGSANGTTSNDRTNYFETLPSNELALGLWLEADRMRSLSITQENFENQRMTV
                     MEERRQRIDNQPYIPSMLRINELAYGDYWPYAHSTIGDMQDLLDAPLEAVQAFFDMYY
                     APNNAVLSISGDFNLDEAMQLVEKYFGDIPARTTPIYTEPAPPEQTAERTETMYDANA
                     TLPAFHIAYHIPQNREADHYALEILALVLGDGESSRLYQTLVKQKEICQDVAVGTDDR
                     RGPDLFSVWAVMASGHAPKAAQDIVIAELESIATEGVSARELEKAKNQIHAAFVFGLQ
                     SNMARSQYLAEFELYYGDANLLKLELDHYLAVSEGDIKRVAETYFKPSQRTVLDVLPG
                     EAPASEAK"
     gene            5105..6658
                     /locus_tag="VFG22_02980"
     CDS             5105..6658
                     /locus_tag="VFG22_02980"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET6415235.1"
                     /translation="MGHRTRSPFLLVVALGAMFWLPACGGSKGSTSTELKSAKDAQAE
                     SQPELESPPAAGETKDMSLPPVHVAELGNGMQVNTIVTDQLPVVYATLVVRSGAESDP
                     AKLPGLSGLVAQMLKEGTSERSSAQIAEEIEFLGADLWTSSDEENTYVGVRALAGQFD
                     VVMGILAEVAGKPSFSNQELQKLKRRELDRLALAEREPSYIARRTFYRNLYGDHPYGT
                     VDTTPQAVKRARRQDLLRWHQEHFVGKNSFLVVAGDVDPDQVDEAAQKLFGDWKAGKR
                     AAPTYATPPSRSDRTILVVDRPGSVQSVIYMGNLAIARANREWIPLMVSNQVLGGSAA
                     SRLFMDLREKRSLTYGAYSAIGERVEVGPFVAYASVRTEVTREALGAFFEHLEVIHEE
                     AASPEELNNAKRFLSDSFPLQVDTPGKLAELVVDLRTFGLPDDYWDNYRRAIRATSAS
                     EALYVARNYIRPSDSLVVVVGQAADFARSLQEFGPVTVVSPDGELKAKFEDKRPNAGE
                     TGAQRGAID"
     gene            6668..8500
                     /gene="glmS"
                     /locus_tag="VFG22_02985"
     CDS             6668..8500
                     /gene="glmS"
                     /locus_tag="VFG22_02985"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HET6415236.1"
                     /translation="MCGIVGHVGDEQATPVLLDGLRRLEYRGYDSAGLAVHSRNGIEI
                     VRAVGKLRNLEKALLKNGLPGTVGIGHTRWATHGRPTELNAHPHVAGPIAVVHNGIIE
                     NHSALRNELVASGYTMSSETDTEIIAHLIHRETKSGLDMLSAIRRSLTFVEGAYAIAV
                     LSEEEPDRIVVAKNASPLVLGISKGATYCASDIPALLPYTNEMVFLEDGEIAVLEATG
                     FALSKLDGTPLTRRPKKISWSPVMAEKAGYKHFMLKEIFEQPRALEDTLRGRLDRERA
                     DIATPEIGLSDDDAKRIKRVILLACGTSHHATIAGRYWIEELAKVPAITELASEFRGR
                     DAVVGEGDLVIAVSQSGETLDTLMAAKAAKERGAKVLAIANVNGSAIPRMADGSFYTH
                     AGPEIGVASTKCFMTQLANLLMFSIWLGRRRGTIDDAQATELVEGLARIPNLIAERLR
                     ETHLMITDIVRDFADARDVLFLGRGLSFPMALEGALKLKEVSYVHAEGYAAGEMKHGP
                     IALLDAHVPVIVLMPQDRHYDRVSSNMQEARAREAKIIGVTTEGDKQGQALCDRFVEV
                     ANTHEALSPFVTVVPLQLYAYCVADHKGTDVDQPRNLAKTVTVE"
     gene            8513..9643
                     /locus_tag="VFG22_02990"
     CDS             8513..9643
                     /locus_tag="VFG22_02990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6415237.1"
                     /translation="MRVGLVTTGFPRFEGDCSGAFLLTLARGLVSNGHSVRVLAPEPR
                     RRRDTPRWPGIELSWVPYARPRSLQRTFYGSGAPDNLRLQPGRWAGAASFIAALHRAS
                     ARELSDCDALVSSWCLPSGWVASKNAAGRAHLCICHATDVRWLSKMPAGGAIAREILA
                     GASSLWFLSTALRDRFFARAGLDPSRTTCHVGSMPIEAPRHLSETRDRIRQRLGIQGF
                     TLLFLGRLVPVKGLDRLLRALAELPEPVGIRVAGDGPERPRLQALARRLGIDAGFEGW
                     VSGERKEVLLRACDAIVISSEPEDGLPTVLFEAKARGLPIISTAVGAIPENLGSHPDA
                     LLVPPHDPRALARAIQQLRARHEEAQPPLKARPAGFLWAGGS"
     gene            complement(9600..10298)
                     /locus_tag="VFG22_02995"
     CDS             complement(9600..10298)
                     /locus_tag="VFG22_02995"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HET6415238.1"
                     /translation="MKEVGAIFCSLGVLALGMPAAAEDGAPLDPAVVVGLVQSFYDQT
                     KTLQAEFEQTRYTRLYDRYDRARGKVVFKKPGMMRWDYAEPNGQVFVSDGKKLLIYQP
                     PEEGEKNGQLIERALEEDQLPSAFSFLIGSGNLEKDFEVRLLEHDDEKFQDGYVLQLV
                     PRKPTPNYEQLVFYVRTLTTGTKRAGIVQRVLIIDSSGNRNRFDFSKIKFNRDVSDKR
                     FNYRPPKGTQRVSP"
     gene            10489..10683
                     /locus_tag="VFG22_03000"
     CDS             10489..10683
                     /locus_tag="VFG22_03000"
                     /inference="COORDINATES: protein motif:HMM:NF016231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HET6415239.1"
                     /translation="MSKHHARIIPPERQKSHLEKKHAELAERIEALDGRLSLTPSEEL
                     ELQQLKKQKLWAKDALEDVG"
     gene            11242..12465
                     /locus_tag="VFG22_03005"
     CDS             11242..12465
                     /locus_tag="VFG22_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415240.1"
                     /translation="MLRTDAFCGTCGEAVPGGKPVVPIARESRQSGVQSAPDRMSGAF
                     APPSATAASARAMAVVAISDPDLAESRDAEVNRTGILQREPLSEPAPVLPLNRKKNGT
                     ESERGEEPMAAESGPSAAQASIPDRDTSLPNIPIPAGPPILASDLLREQMRPASPGEK
                     SLRRTAVGLSALGAAAVLFAGGVHPLTFVSLALLITMVTLALTPMSYRGRAIMLFMVG
                     TVATGVALWQQAIHAIAPGGVILAAAAILLSGSLLFRAYYRGASLARVAVTLGVLALG
                     TWFVLSGGHESLVMLDGNWHSWAPASTQMTLALLALLSLMAFMDSSTRGGSHVWAVAV
                     LVVYSIHIGLLVASEVWPVVPKPAAIEGPAIAAIIAGLVGTVVAGMALAQVLVVVYQS
                     ALNRVKAKASASAAP"
     gene            complement(12468..13511)
                     /locus_tag="VFG22_03010"
     CDS             complement(12468..13511)
                     /locus_tag="VFG22_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HET6415241.1"
                     /translation="MLRKTILPCVMLLAIHGAAGADQNTEAPPDSAADAAPEKPENLC
                     IKSPKLESVARELADLQDIPPSGELIRRVREAGVDANPVYAKFGVTGEFDSFKAWLKD
                     LDEAADAPLICGRARKGERIVLVAAVRAGQLTVQGQQRLHAEVIEDFRDPYLVVRDPK
                     GSSRRIAVDGGEFGATITIPIEWGRPLFVQLVATGPNGPRPVAERWVGKIPEGEPTRN
                     GSQSPEAWLMQLRRAAGARTLRSNRLLSQEATSHAQSICDSGKLGHDLDPGGDPEARL
                     LKRGIEARVVGEAVARAATMRDALHAIEDSPSHRLTVTDPRFTDAGYGQAKDDRGRTC
                     AVILLASWPRTVP"
     gene            complement(13521..14711)
                     /gene="argJ"
                     /locus_tag="VFG22_03015"
     CDS             complement(13521..14711)
                     /gene="argJ"
                     /locus_tag="VFG22_03015"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:TIGR00120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HET6415242.1"
                     /translation="MSGRVKGFRFAGVSGGVKASGALDLGLLLADEPAVASGVFTKNQ
                     VRAAPVVISERRLASGRCQAILVNSGNANACTGKEGRKAALALTRAAAVAVHVPRALV
                     VPASTGVIGVQLPRDAIEASIPALVDDLSEAGAARFARAIMTTDRGPKVASAEVKIGR
                     TVCRILGIAKGAGMIHPNMATTLAFVTTDAPLARAPLSRILRRATEVTFNRATVDGDT
                     STNDSIYALASGAATTRMVEEKTSDGRRFEAAFTEVLESLATKIVADGEGAEHLVRIE
                     VRGTRSDADAVQLARTIASSQLVKTALHGCDPNWGRILAAAGRSGARFNPDQVSVRIG
                     PVSIFERGTPTMTAKTEAKAIAVMKRAEYEISVTAGTGRGLGHYWTCDLGHEYVRINA
                     AYRT"
     gene            complement(14736..17918)
                     /gene="secA"
                     /locus_tag="VFG22_03020"
     CDS             complement(14736..17918)
                     /gene="secA"
                     /locus_tag="VFG22_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HET6415243.1"
                     /translation="MFNSALKKVFGTKHAREVKRMQPMVAAISALEDETKKLSDAQLR
                     GKTAEFRQQLDNGATVDELLVPAFAVAREAGKRVLGMRHYDVQLVGGIVLHQGKIAEM
                     KTGEGKTLVATLPCYLNGLEGKGVHVVTVNDYLASRDAEWMGKLYGFLGLSTGVVVPG
                     QSNQLKKRAYNCDITYGQNNEFGFDYMRDNMKFSIYDYAQRELNYAIVDEVDSILIDE
                     ARTPLIISGPGETASEKYEMINEVIPRLRKEEHFDLDEKNHSVTLTEEGMERAQELLG
                     GRGLLGGDGNLYDPANLETLHILQQCLRAHALYHRDQHYMVTEDGKVMIIDEFTGRVL
                     AGRRWSDGLHQAVEAKERVLIQSENLTLATISFQNLFRLYKKLSGMTGTADTEATEFH
                     NIYKLDVVVIPTNKPIARIDEEDLIYKTEREKFRAVCQEIEESYKRGQPVLVGTTSVE
                     KSDAVAHFLTRQGVPHHVLNAKQHEREAYIVAQAGTPGAVTVATNMAGRGTDIVLGGN
                     AEMLARIEVLETAPEDVLSDPERREAAIAKNTELYISKCKEDAKRVKDAGGLQIIGTE
                     RHESRRIDNQLRGRSGRQGDPGASRFFLSLEDDLMRIFAGERVQAMMDRLGMEEDVPI
                     EHRWVTRAVENAQKKVEERNFDVRKHLLEYDDVMNQQRKSTYALRKQVLRGEYRTVPT
                     DDERKAGVESQPLVEGIDEALLSRVSKVLENMVKFHSYPLPEAGLTQDEVPQHRKKAI
                     ESDLSTLTDVRVQLLEKDVYLWFGCVVPLEAFRKDPAGVYEHLQDAVGMSLTEQKERL
                     LDLVDEIVSTMADHACPPGKHYEDWDLQGLERAYKEQFGLSATGIHEISDRDELLRKL
                     YGDASAVLERKEQEFGTENFLRLFRDLYLQEIDKQWMDHLQGMDHLRDGIGLRGYGQR
                     DPKKEYQREGFDMYLEMVQSVKSSVTLGMFTVERAGEEELRQLEEQRRQASEKRQRQI
                     RASHQLGDEQAGDRGSQSGPSRQARRAAARRGRRTGGSPGAGAPSPQQAATVTRDRPK
                     LGRNDPCYCGSGKKYKNCHYREDQAAASPQ"
     gene            complement(17961..>19461)
                     /locus_tag="VFG22_03025"
     CDS             complement(17961..>19461)
                     /locus_tag="VFG22_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415244.1"
                     /translation="LSIPITRQERQFNGFAWAIRMSPLLVAIILATLWIAGTPPEVPD
                     LRLSAPLVAQPGTSIGLRAWQLAEDENGYPVILAPNVLVELQNEVGLVLAKTELVESL
                     VQGREGRLSIPAGVDEVLTLVAHAEIDGRDVSVERALYVQEGIESRLPRGREVNAFQV
                     YELGPIRLASSRRSIAALDPRVEEGACVPDLRCWLLVWVADEEGRVRVRPLAGVRLEQ
                     GTFASTDGFVRIPLLVGGTEARVEVELFSEDGDLIAAREARLPLVPGGIVVRISEEKE
                     RIRLDWEQLGARGPVLVDAFQGHRWVRAFSLAPEHPYLPAFGPGVWRLQVRSDLFSDN
                     TAAVQYLVIADPGGPGRARQAADAVLAEADREGLDPIAVAVIEGTLQVEDEQTLERAL
                     FAVPSFDVVSTGAGASARVGAHGTLESEQELRRWQAAAVILLIGLMVSMVLLRVELLA
                     QARARQLLDELGDGFTSPSRGSSSGRGLWAFVLLVFVLMALLALSKRWF"
BASE COUNT         3605 a         6199 c         6002 g         3655 t
ORIGIN      
        1 cgcacactgg aggcccgcaa agcgtcggac gccggcaaga gctacaccaa ggcgctgctc
       61 gacgccggac ccacgaagat cgacgcaaag cttcgggagg aggccgcaga gctgggtcag
      121 gctctcatcg gcgaaagcga tgagcgggta gcctccgaag caggcgatgt gatctaccac
      181 ctcctggtcg ggctggtctc acgtggggtc tctcttcgcg agctcttcag cgagctttcc
      241 aagcggttcg gccagtcggg acacgaggag aaagccaagc gctaaccccc gcccgagtcg
      301 actgtcgcca aaggcgcgct ctcgcccctc tcagtgcagg atctcgtcga tatcgcgctc
      361 gatcgtttcc tcgcggaccc ggcgggccag cgcgagctcc gtgatcaaga gctttcgggc
      421 ttgctccatg agcttctttt cgccgtagct gagctcgcct ttgtcggcgc gtaccttgga
      481 caggtcgcgg agaaccttgg cgactttgat gggggagccg ctcttgagca tctcggtgta
      541 ctcgcggtag cgccggttcc agggctgcga ggtgacggcc atttcgtcct tcttgagctc
      601 ttcgaggacc gtcttggcgg cgctcctcga catgacctgc cgaagcccga cccgatcgac
      661 cgcgttcaat gcgacgcgga tcgtgccgga tttgccgttc accagtttga gaatatagaa
      721 atcgatccgg tcggagccga tcaagagctc ttcaatttgc gtgatctctc cgacaccgtg
      781 tgctggatga actgctttgt cgcctacgct aaagctcatg gggtaactct ccttcgagtc
      841 aaaaacgacg aaaacccgcg ccgggagcgc gggcagagaa ctgttcgtcg ctgagtgtgc
      901 aaacaactac atgcacagga tctgtatagc aaaaccggac tgaaaagtca atgcttactt
      961 ctgcgcaaac atgtgtgatt tcagtggttt gtggctattt tgtcgaccga tttcaggctt
     1021 gacaaaacgc accttggaat gcgcaggaaa agaggctcag tttggccgcg ctcgtttgct
     1081 cgccgccgca gccggggccg ccttggtttc agccttggtt tcggtctgag cctcggcctt
     1141 cggtgcggca ggcgtgtcct cttcgatgag cccgtgctgc tgaaggagct tgcgctcgac
     1201 cgcggcaaga acgtcaggat gctcttcgag gaaggtccgg gcgttgtcac ggccctggcc
     1261 gacgcgctcg tcgccgtagc tgtaccaggc acccgacttg ttgatgatgc ccgcttcgac
     1321 gccgagatcg agcacatcgc cagtgcggct gatgccgtgt ccgaaaagga tatcgaactc
     1381 gcaaagacgg aaaggaggtg cgagcttgtt cttgacgacc ttgacgcgac agcggttacc
     1441 cactgcagca tcgccgacct tgatcgtgcc gatgcggcga atgtcgagac gctgcgaggc
     1501 atagaacttg agcgcgtttc cgccgctggt cgtttcgggg cttccgaaca tcacaccgat
     1561 cttcattcgg atctggttga tgaagaacag ggaggtgtga ctcttctgga ggttgccggt
     1621 gagcttccga agcgcctggc tcatcaagcg tgcctgaaga ccgacatggc tgtcgcccat
     1681 ctcgccctcg atctctgcgc gaggcaccag ggcggccacc gagtcgatga cgatcatatc
     1741 gacggcgccc gagcggacga gggtgtcggc gatctcgagg gcctgctcgc cgttgtcggg
     1801 ctggctgacc agaagctcgt cgacgttgac gccgagctta cgggcatagt ttgggtcgag
     1861 ggcgtgctcg gcgtcgatga aggccgcgat gccgcccttt ttctggcact cggcgatcgc
     1921 atggagggtc aaagtggtct ttccgctcga ctctgggccg tagatctcga tgatgcggcc
     1981 ccgtggatac ccccccacgc caagagcgag gtcgagggaa atcgccccgc tcgaaatcac
     2041 cggtacctgg attttcgccg attcatcgcc taaacgcatg atcgcgcctt ttccataggc
     2101 tttttcgatg gttccaaggg ctaggttgag ggctttttcg cggttcgagt cggccatggc
     2161 gtgatttcct ttgggtgatt tacctgaaca cccgtctagt cggcttcatt acagtagtca
     2221 agttcatgcc cgaaggcgtg catgaacccg agcctaccgc ggccaggccc aggccgcgag
     2281 agcagaacga ctgggcccgt gcgatgaggg gcaggtggat tcgcccgagt cgggcctgcc
     2341 tgaagcagcc tcagcgctcc ccgagatcga agtcgcgacc tagcgccgcg cggcgaacga
     2401 gctcgagagc gatgtaggcg gccaggcgct gaacgcgatc tcgtccccag ggcagcttcc
     2461 tcagcttggt gatcgtcggc tcgcccttgc gcgccaggcc aaaccagatc gtgcccacgg
     2521 gcttgtcgtc ggtacccccg ctcggccctg cgacgcccgt gatcgacacg gcgatatcag
     2581 cacctgcgac ccgaagcgca ccctctgcca tcgccgccgc ggtctccgag ctcaccgcac
     2641 cgtgggcgcg caaggtttct tcgctcacac cgagaaccgc aaccttggcg ctgttcgcgt
     2701 acgcgactgc atcgagcaga aggtaatccg aggcgcctgg cacgcgcgtc aacatctctc
     2761 ccacccgtcc gccggtgcac gactccgcga cagcgagtgt ctttcctttg ttgcgaagcg
     2821 cctcgcctac catttcggga aacgagtcat cgcgtcctcc aaacaccgca tcgccgagac
     2881 gttcgcggac ctgttccgcg atcgactgag aacgccgctc ggcttccgca gcagactccg
     2941 cgcgcacgag aaccttcacc tcgatttccg ggaagtgtgc gcgatagcca agagtcaacc
     3001 cttcatgctc cttctccagg tcacgaaggg cttcggcgac accggactcg gtcatcccaa
     3061 aggttcggag atgctcttgg tgcgttcgtg gttccgccag cggcgcgatg agcggcacga
     3121 tcgtctcgaa gaagatgtgc ttcatctcac gaggcacgcc cggaacgaaa aagaaacggc
     3181 agtcgccgag agtcatcgtg aagccaggtg cggttcccat ggcgttcggt agaatcgtgg
     3241 cgcccactgg gaaatcgcct tgcttggcat tggactcggg catgactctg ccaaacgccc
     3301 tgaacttttc tcggatcccg gcgacaacgc tttcatcgcg gcgaagccct acccgcgcgg
     3361 ccttcgccgc ggcttccgtg gtgaggtcgt ctgaggtcgg cccaagccca cccgtggcca
     3421 ctacgacgcg ctgctctttg gcaaagcgtc ttacaatcgt cacgattcgc tcgagcgagt
     3481 cgtccaccgt cgcgtgctcg atgacgtcga acccgagctt ggtgagctcc tctcccagcc
     3541 aggcggcgtt gctgttgacc aactcaccac gtgtgagctc ggtcccgatc gataagacgg
     3601 cggcggtcat ggcggtcgaa gagtgttcgc aaaacccgat cagcagtcaa ccgctttgcc
     3661 cctaggggcg gatgtggtag cgaggctgga agcttttccg cgcttcgcat gttgatggga
     3721 gtcagatgaa ccggattttc aaggctacag cattggggtt gctggtcgcc gccctaactc
     3781 tttctgcggg aagacccgtg gttgccgagc aaagcacggc tgcgctctcc aacttgcagg
     3841 tgagccgcgc caagctcgac aacggtctgc gtgtcgtgat gaaccaggac cgtacggttc
     3901 cgacggtcgc cgtctccgtc tactacgacg ttggctctag aaacgaagtc aagggccgct
     3961 ccggcttcgc gcatttgttc gagcacatga tgtttcaggg atcggccaac gtcggcaaag
     4021 gcgagcactt cagcctgatc atcaaccgcg ggggcagcgc gaacgggacc accagcaacg
     4081 accgcaccaa ttatttcgaa accttgccat ccaacgagct cgcgctcggg ctctggctcg
     4141 aggctgaccg gatgcggtca ttgtcgatca cacaggagaa cttcgaaaat cagcggatga
     4201 ccgtcatgga agagcggcgg cagcgaatcg acaaccagcc gtacatcccg agcatgcttc
     4261 gcatcaacga gctcgcctac ggcgactact ggccgtacgc ccactcgacc atcggcgaca
     4321 tgcaggattt gctcgacgcg cctctagagg ccgtccaggc attcttcgac atgtactacg
     4381 cgcccaacaa cgcggtgctc agtatcagcg gtgacttcaa tctcgacgag gcgatgcaac
     4441 tggtcgagaa gtactttggc gatatcccag cacgcacgac ccccatctac accgagccag
     4501 ctcctcccga gcaaacagcc gagcgcaccg aaacgatgta cgacgcgaac gcgacgctcc
     4561 ctgcgttcca catcgcgtac catattccac agaaccgcga ggccgatcac tacgcgctag
     4621 aaatcctcgc tctggtcctc ggcgacggcg agtcgagccg gctctatcaa acgctcgtga
     4681 aacagaagga gatctgccaa gacgtcgccg tgggaaccga cgaccggcgc ggccccgatc
     4741 tgttctcggt gtgggcggtg atggcaagcg ggcacgcacc gaaggctgcg caagacatcg
     4801 tcatcgccga gctcgagtcg atcgcaaccg agggcgtgag cgcgcgggag ctcgagaagg
     4861 cgaaaaatca aatccatgcc gcgttcgtgt tcgggctgca atcgaacatg gcgcgttcgc
     4921 agtatctggc cgagttcgag ctctattatg gcgacgccaa cttgctcaag ctcgagctcg
     4981 accactatct ggcggtctcc gaaggcgaca tcaaacgggt cgccgaaacg tacttcaagc
     5041 cgagccaacg caccgtgctc gacgtcttgc ccggggaagc tccggcatcg gaggcaaagt
     5101 gaccatggga catcgaaccc gatcgccgtt cctgctcgtc gtggccctgg gcgcgatgtt
     5161 ctggctgcct gcctgcggtg gctcgaaggg aagcacgagc accgagctca agtctgccaa
     5221 ggacgcccaa gccgaatcgc agccggagct cgagtctccg ccggcagcgg gcgaaaccaa
     5281 ggacatgtcc ctcccgccgg ttcacgtggc cgagctcggg aacggcatgc aggtgaacac
     5341 catcgtcacc gatcagctgc ctgtcgtata tgcaacgctc gtcgttcgga gcggcgcgga
     5401 atcggatccc gcaaagcttc cgggtctgtc cggtctcgtc gcccagatgc tcaaggaagg
     5461 cacgagcgag cgctcgagcg cgcagatcgc cgaggagatc gagttcctgg gtgccgatct
     5521 ctggaccagc tcggacgagg agaacacgta tgtcggcgtt cgggcgctgg ccgggcagtt
     5581 cgatgtcgtc atgggcatcc tcgcggaagt cgccggcaag ccgtcgttca gcaatcagga
     5641 gctacagaag ctcaagcggc gcgagctcga tcgtcttgcg ctcgcggagc gagaaccaag
     5701 ctacatcgca cgacgcacgt tctatcgaaa tctctacggt gatcacccgt acggaaccgt
     5761 ggacacgacc cctcaagcag tgaaacgagc tcgacggcag gacttgctgc gttggcacca
     5821 ggagcacttc gtcggcaaaa actcgttcct ggtggtcgca ggcgatgtcg atcccgacca
     5881 agtggacgag gcggcccaaa agctcttcgg cgattggaag gccggcaagc gcgccgcccc
     5941 aacctacgcg acgccgccct cgcgcagcga ccgaaccatc ctggtcgtgg atcggccggg
     6001 ctccgtccag tcggtgatct acatgggcaa cttggcgatc gcgcgcgcga accgcgagtg
     6061 gatccccttg atggtttcga accaggttct cggcggatcg gcggcgtccc gactcttcat
     6121 ggacctccgc gagaagcgaa gcctgaccta cggtgcgtat agcgcaatcg gcgagcgggt
     6181 ggaggtgggc cccttcgtcg cctatgcatc ggtccgcacc gaagtgaccc gcgaggccct
     6241 tggagcgttc ttcgagcacc tcgaggtcat ccacgaagag gcggcaagcc ccgaagagct
     6301 caataatgca aagcggttcc tcagcgattc gtttccgctg caagtcgata cccctgggaa
     6361 gctcgccgaa ttggtcgtcg atttgaggac ttttggcctg cccgacgact actgggacaa
     6421 ctaccgccgg gcgattcgcg ctaccagcgc atctgaggcg ctgtacgtcg cgcgaaacta
     6481 catccggcca agcgactccc tggtggtggt cgtgggtcag gccgcagatt tcgctcgctc
     6541 gcttcaggag ttcggaccgg taaccgtcgt ttcgccagac ggcgagctga aggccaagtt
     6601 cgaggataag cgtccgaatg cgggcgagac cggtgcgcag cgcggtgcca tcgactaaag
     6661 tcagggcatg tgcggaatcg ttggtcacgt cggtgacgag caggcgaccc ctgtactgct
     6721 cgatgggctg cggcggctgg agtatcgggg ttacgactcg gcgggtctcg ccgtgcattc
     6781 gcgcaacggc atcgagatcg ttcgcgcggt gggcaagcta cgcaatctcg aaaaggcgct
     6841 gctcaaaaac ggcctcccag gaacggtcgg gatcggacac acacggtggg cgacccacgg
     6901 ccggccgacc gagctcaacg ctcacccaca cgtcgcgggt ccaatcgccg tcgttcacaa
     6961 cggcatcatc gagaaccact cggcactccg caacgagctc gtggccagcg gctacacgat
     7021 gtcgagtgaa accgacaccg aaatcatcgc acatctgatt catcgagaaa cgaagtccgg
     7081 gctcgacatg ctcagtgcga ttcgtcgctc cctcacgttc gtcgaaggcg cctatgccat
     7141 cgccgtcctt tccgaggagg agcccgaccg aatcgtcgtg gcaaagaacg cctctcctct
     7201 ggtactgggc atctcgaagg gcgcgaccta ctgcgcgagc gacattcctg cgctactccc
     7261 ctacaccaac gagatggtgt ttctcgagga tggtgaaatc gccgtgctcg aagctaccgg
     7321 cttcgcgcta tcgaagctcg atggcacgcc gctcacccga cgccccaaaa aaatatcctg
     7381 gtctccggtg atggccgaga aggccgggta caagcacttc atgctcaagg agatcttcga
     7441 gcagccccgg gcgctcgagg acactctgcg aggtcgcctc gatcgtgaaa gagcggacat
     7501 cgcgacgccc gaaatcgggc tgagcgacga tgatgccaag cggatcaagc gcgtcattct
     7561 cctggcgtgc ggcaccagtc accacgcgac gatcgcggga agatactgga tcgaagagct
     7621 cgcaaaggtg cccgcgatca ccgagcttgc gagtgaattc cgaggacgtg atgccgtcgt
     7681 cggagagggc gacctggtca tcgcagtgag tcagtctggt gaaacgctcg acacactcat
     7741 ggccgccaaa gcagccaagg agcgaggtgc caaggtcttg gccatcgcga acgtgaacgg
     7801 aagcgcaatt ccacgaatgg ctgacgggag cttctacacg cacgccggtc cagagatcgg
     7861 ggttgcatcc acgaagtgtt tcatgaccca actcgccaat ctgctgatgt tcagcatttg
     7921 gctcggtcgc cgacgcggaa ccatcgacga cgcccaggcc accgagctcg tcgaagggtt
     7981 ggcacgtatc ccaaacctca tcgcagagcg ccttcgcgaa acgcatctga tgatcaccga
     8041 catcgttcgc gacttcgcgg atgcgcgcga cgtgctcttc ctcggacgcg gactgagctt
     8101 cccgatggcg ctcgaaggcg cgctcaagct gaaggaagtc tcgtacgtgc acgccgaagg
     8161 ctacgctgcc ggcgaaatga agcacggccc gatcgcgctc ttggatgcac acgtgccggt
     8221 gatcgtgctg atgccacagg accgacacta cgatcgggtc tcgagcaaca tgcaggaggc
     8281 ccgcgcccgt gaagcgaaga tcatcggcgt gacgacggaa ggcgacaagc agggacaggc
     8341 gctctgcgat cgcttcgtcg aagtggcaaa tacgcacgag gcgctgagcc catttgtcac
     8401 ggtcgtgcct ttgcagctct acgcgtactg cgtggcggac cacaagggca ccgacgtgga
     8461 ccagccacga aacctcgcca agaccgtcac cgtggagtga gtcgggcgag ccatgcgagt
     8521 cggtctcgtc acgacgggct tcccgcgctt cgaaggtgac tgcagcgggg cgttcctgct
     8581 gaccttggcg cgcggcttgg tgagcaacgg gcattcggtc cgggtgctcg ctccggagcc
     8641 gcggcggcgt cgcgatacac cacggtggcc gggcatcgag ctgagctggg tgccctacgc
     8701 gcgaccgcgc tccctacaac gaacattcta cgggagtggc gcgccggaca acttgcgctt
     8761 gcagccggga cgctgggcgg gtgcggcgag cttcattgct gcgcttcatc gcgcgtctgc
     8821 gcgggagctg tccgactgtg atgcgctagt cagctcctgg tgtctgccaa gcggatgggt
     8881 cgcctccaag aacgccgccg ggagggccca cctatgcatt tgccatgcaa cggacgttcg
     8941 ctggctctcc aagatgccgg cggggggcgc gatcgctcgt gaaatcctcg cgggggcgag
     9001 ctcgctctgg tttctgagca cagcccttcg cgatcgcttc tttgctaggg cgggcctgga
     9061 tccttcgcgg acgacttgcc acgtcgggtc gatgccaatc gaggcaccgc ggcatctgag
     9121 cgagactcgc gaccggatcc gccagcgtct cggcatccag ggtttcaccc tgctcttcct
     9181 agggcgtctg gtaccggtca aagggctcga ccgactgctc cgtgcgctcg ccgagctgcc
     9241 ggagccggtc ggaatccgtg tggccggcga cggcccggag cgtccccgcc tccaagcact
     9301 ggcccggcgc ctcgggatcg atgccggctt cgagggctgg gtgagcggcg aacgcaagga
     9361 ggtgctcctg agggcgtgcg acgccatcgt catatcgtcc gagccggaag atggacttcc
     9421 gacggtgctc ttcgaggcca aggcccgcgg tcttcccatc atcagtaccg ccgtgggcgc
     9481 aatcccggag aacctcggga gtcatccgga tgcgctgcta gtgcccccgc acgatcctcg
     9541 cgcgctcgct cgggcaatcc agcaactaag ggcgcggcac gaggaagctc agccgccgct
     9601 caaggcgaga cccgctgggt tcctttgggc gggcggtagt tgaagcgctt gtcggacacg
     9661 tcccggttga acttgatctt tgagaagtcg aagcgatttc gattccccga cgagtcgatg
     9721 atgagcacgc gctgcacgat ccccgctcgc ttcgtgccgg tcgtgagcgt ccgaacgtag
     9781 aacaccagct gttcgtagtt gggcgtcggc ttgcgaggta cgagttgcaa gacgtagcca
     9841 tcctgaaact tctcgtcgtc gtgctcgagc aagcgaactt cgaagtcctt ctcgaggttg
     9901 ccgctgccga tcagaaacga aaacgccgac ggcaattgat cctcttcgag ggcgcgctcg
     9961 atgagctggc cgttcttctc gccttcctcg ggcggctggt agatcaaaag tttcttgccg
    10021 tcgctcacga acacctggcc attgggttcc gcgtagtccc agcgcatcat cccaggcttc
    10081 ttgaagacga ccttccctct cgcgcgatca tagcgatcgt agaggcgggt gtaccgagtc
    10141 tgctcaaact cggcctgaag agtcttggtt tggtcataga aggactggac caggccgacg
    10201 accaccgcgg ggtcgagcgg cgcaccgtcc tcggccgcag cgggcattcc gagcgccaag
    10261 acccccaaac tacaaaagat cgccccaact tccttcatca tcagccttca gaacgctccc
    10321 gctaggcaag tattcaccgt gaccgcgtgt gcacaaggca accacaaatc gtccaaaagt
    10381 gggattgatc aatgcgctca agatccgtag gatacggaag aggcgcgcgt gcgcgccggg
    10441 cgcttgcttg agttagcttt ggaacccttg catcacctcg gaggtgacat gtccaagcac
    10501 cacgctcgaa tcatcccgcc agagcgccag aagtcgcacc tggaaaagaa gcatgctgag
    10561 ctcgcagagc gcatcgaagc actcgatggc cgcttgagtc tcaccccgag cgaggagctt
    10621 gagctccagc aactcaagaa acagaaactt tgggccaagg acgccctaga ggacgttggg
    10681 taggcccttc gaggacctgc tcgtagagac ccttcttttc tccataccac cctaaacccc
    10741 gtgcgaacgg cccgtctccg caggcgggcc gttccttttt ggtcctgccc cgaatcccaa
    10801 aagcaaaatg ggccaccaac cggtgaccca tttcaaacga aagaatcggt gctaaagtga
    10861 agaagctagg gtaagtaagt taagccgaga tttcctgagt gttttcaaag ttaaggacga
    10921 cccccgccgc gcggatgccg ggcaagctgg aaagtgcgcg gcgagggtcc taagcaagcg
    10981 atgggctcta ctgcaatcgc gatgccaact tgtcacggcc tgaaaacgca gcgatttaac
    11041 cgccaccaag gctatcggta cgacatttct gtcatagaca tggaaccgat tgtcgcggtt
    11101 gaagcaaaag gaggcgtttc ctgtccgata ggcgtacatc cccctacggc gccggttgaa
    11161 gtgcgcgggt tctcccgcgt atgatctgag cgaccggaca ggccagtcac catgccgaat
    11221 tgcaatagct gcgacgctac cgtgctgaga acagatgctt tctgcggcac ctgtggggag
    11281 gccgtgcccg gtggaaagcc cgtggttccg atcgcgcgcg agtcgcggca gagcggagta
    11341 caaagcgcgc cggatcgcat gagcggcgct ttcgctcctc catccgcaac ggccgcttcg
    11401 gcgcgagcca tggcggtcgt tgcgatctcg gatccggact tggccgagtc tcgcgacgca
    11461 gaggtcaacc gcaccgggat cctccagcgt gagcctctga gcgagccggc gccggtgctc
    11521 ccgctcaacc gcaagaagaa cggtaccgag agcgaacgcg gtgaagagcc gatggcagct
    11581 gagtcggggc cgagcgcggc gcaagcatcg atccccgatc gcgacactag ccttcccaac
    11641 atacccattc cggcgggccc accgattctc gcgtccgatc tactgcgcga acagatgcgg
    11701 ccagcgagcc ccggcgagaa atcactgcgc cgcaccgcgg tggggctctc ggctctaggc
    11761 gcagcggcag tcctgttcgc cggcggggta catccgttga catttgtctc gctggccttg
    11821 ctgatcacca tggtgacctt ggcgctcacg ccgatgtcgt accgaggccg ggcgatcatg
    11881 cttttcatgg tcgggaccgt agcgaccggc gtcgcactct ggcagcaagc cattcatgcc
    11941 atcgcacccg gcggcgtgat cctcgcagcg gcggccattc tcctgagcgg gagcctcctt
    12001 ttccgcgcct attaccgcgg tgcaagcctt gctagggtcg ccgtgacatt gggcgttctg
    12061 gccctcggca cctggtttgt cctatcgggc ggccacgaaa gcctggtcat gctcgatggc
    12121 aattggcatt cctgggcccc cgcgagcacc caaatgacac tggctcttct cgcactgctg
    12181 tcactgatgg ccttcatgga cagcagcacc cgaggcggct cgcacgtctg ggcggtcgct
    12241 gtgctcgtgg tgtattcgat tcacatcggg ctcctcgtgg cgagcgaggt ctggccggtc
    12301 gttccgaagc ccgccgctat cgaaggtcct gcgatcgcgg cgatcattgc ggggctggtg
    12361 ggaaccgtcg tcgcaggcat ggcgctcgcg caggtcctcg tggtcgttta ccagtcagcc
    12421 ttgaatcggg tgaaagcgaa ggcaagcgct tccgccgcgc cctgatctca ggggaccgtg
    12481 cgcggccagg atgccagtag gatcacggcg caggttcgcc cccgatcgtc cttggcctgg
    12541 ccatagccgg catcggtgaa ccgcgggtcg gtcacggtga gtcggtggct ggggctgtcc
    12601 tcgatcgcgt gaagcgcatc ccgcatcgtg gcggcgcggg ctaccgcttc acccactacg
    12661 cgcgcctcga tgccccgctt gagcagcctc gcctcgggat ccccacctgg atcgagatcg
    12721 tggccgagct tccccgagtc acagatcgat tgcgcatggc tcgtggcctc ctgggacagc
    12781 aagcgattcg aacggagcgt gcgcgcaccg gccgcacggc ggagctgcat gagccaggcc
    12841 tctggggatt gcgagccatt ccgcgtgggc tcgccctcgg gaattttgcc tacccagcgc
    12901 tccgcgaccg gccgcggtcc gttgggaccc gtcgccacca gctgaacgaa gaggggacga
    12961 ccccattcga ttggaatcgt gatggtcgcg ccgaattcac cgccgtcgac cgcgatgcgc
    13021 cgtgacgagc ccttggggtc gcggaccacg agatagggat cgcggaaatc ttcgatgacc
    13081 tctgcatgaa ggcgctgctg accctggacc gtgagctgac cggctctcac cgctgcgacc
    13141 agaacgatcc gctcgccctt acgggcacgc ccgcagatca acggtgcgtc cgcggcctcg
    13201 tcgaggtcct tgagccatgc cttgaaacta tcgaactctc cggtgacacc gaacttcgca
    13261 tacacgggat ttgcgtcgac tccggcttcg cgcaccctgc ggatcaattc gccagacggc
    13321 gggatgtcct gtaggtctgc gagctctctt gcgaccgact cgagcttcgg gctcttgatg
    13381 cagaggttct caggcttctc cggagcggcg tcggcggcgc tgtcgggggg agcctcggtg
    13441 ttttggtcgg cgccagctgc cccgtgaatg gcgagcagca tgacacaagg aagaatcgtt
    13501 ttgcgcaaca tcagcttgaa tcacgtccgg taagcggcgt tgatccgaac gtactcgtga
    13561 ccgaggtcgc aggtccagta atgaccaaga ccgcgacccg taccggccgt caccgagatc
    13621 tcgtactccg cgcgtttcat gacggcgatc gctttggcct cggtttttgc ggtcatcgtg
    13681 ggcgttccac gctcgaaaat cgagaccggt ccgatcctca ccgaaacctg atcaggattg
    13741 aaacgtgctc ccgagcggcc tgcggcagcc aggatgcggc cccaattggg gtcgcatcca
    13801 tgaagcgccg ttttcacgag ttgggagctg gcgatcgtgc gcgcgagctg aaccgcatcg
    13861 gcatccgagc gagtaccgcg tacttcgatt cgaacgagat gctctgcccc ctccccgtcc
    13921 gcgacgatct tggtggcaag cgattctagc acctccgtga atgcagcctc gaagcggcgc
    13981 ccatcgctcg tcttttcctc gaccatgcga gtcgtcgcag ctcccgatgc aagcgcatag
    14041 atggaatcgt tcgtgctggt atctccatcc acagtggccc gattgaaagt cacctcggtc
    14101 gcgcggcgaa ggatccgaga gagcggggct cgcgcgagcg gggcgtccgt cgtcacgaag
    14161 gcgagcgtgg tagccatgtt gggatgaatc atgccggcgc ccttggcgat ccccaggatg
    14221 cgacagaccg tccgcccgat cttgacctca gccgacgcaa ccttggggcc gcgatcggtc
    14281 gtcatgatcg cccgcgcaaa gcgcgccgca ccggcctcgg acagatcgtc gacgagcgct
    14341 ggaatcgagg cttcgatcgc atcgcggggc aactgcactc cgatcacccc cgtcgatgca
    14401 gggacgacga gcgctcgcgg cacgtggacg gcgaccgcag cggcgcgggt aagcgcgagc
    14461 gccgcttttc gcccctcctt tccggtacaa gcgtttgcgt tgccgctatt gactagaatc
    14521 gcctggcaac gccccgaagc gaggcgccgc tcactgatca ccaccggcgc agcccgcact
    14581 tggttcttcg tgaaaacccc cgaggcgacc gccggctcgt ccgcgagcag caagccgagg
    14641 tccaacgccc cgctcgcttt gactccgccg cttacgccag cgaagcgaaa acccttcacc
    14701 cgtccactca tggcgtgcgt cttaccagat gcgcgttact gcggcgaggc tgccgcctgg
    14761 tcttcacggt agtggcagtt cttgtacttc ttaccgctgc cgcaatagca gggatcgttg
    14821 cgaccgagct tggggcgatc gcgtgtgacg gtggccgctt gctgcggaga cggtgctcca
    14881 gcgcccgggc tacctccggt acgacgcccc ctccgagcgg ctgcccggcg cgcctgccta
    14941 ctcggtccag attgcgaccc gcgatcgccg gcttgctcgt ctccaagctg atgactcgcg
    15001 cggatttgtc gctggcgctt ttcgctcgcc tggcgacgct gctcttcgag ctgtcgcagc
    15061 tcctcctcac cggcgcgctc gaccgtgaac attccgaggg tgaccgagga cttcacgctc
    15121 tgcaccatct ccaagtacat gtcgaagccc tcgcgctggt actccttctt ggggtcgcgt
    15181 tgcccgtaac cgcgtaggcc gatgccgtcg cggaggtgat ccatgccctg caagtggtcc
    15241 atccactgct tgtcgatctc ctgcaaatac aggtccctaa acaagcggag gaagttctcg
    15301 gtgccgaact cttgctcctt gcgctcgagc acggccgaag catcgccgta gagcttgcgg
    15361 agcagctcat cgcggtcgct gatctcgtga atcccggtcg cgctgagtcc gaactgctct
    15421 ttgtaagccc tctcgagccc ttggagatcc cagtcctcgt agtgcttgcc tggcgggcag
    15481 gcgtgatcgg ccatcgtgct gacgatttca tcgaccaagt cgagcaggcg ttctttctgc
    15541 tcggtgagcg acatgccgac cgcatcttgg agatgctcgt acacgccggc cgggtctttt
    15601 cggaaagctt cgagaggcac gacgcagccg aaccaaagat agacgtcctt ctcgaggagc
    15661 tggacgcgta cgtcggtcag cgtgctgagg tcggactcta tcgccttctt ccgatgctga
    15721 ggcacctcgt cctgggtaag tccggcctcc ggcaagggat acgagtggaa cttcaccatg
    15781 ttctcgagca ccttggacac gcgcgataga agcgcttcgt cgattccctc caccaagggc
    15841 tgcgactcga cgcccgcttt gcgctcatcg tcggtcggaa cagtgcgata ctcgccccgg
    15901 agaacctgct tacggagcgc atacgtgctc ttgcgctgct ggttcatcac gtcgtcgtat
    15961 tccagcaggt gctttcgaac gtcgaagttg cgctcttcga ccttcttctg agcattctcg
    16021 acggcacgcg tcacccagcg gtgctcgatg ggaacgtcct cttccatccc gagccggtcc
    16081 atcatcgcct gcacgcgctc gccggcgaaa atccgcatca agtcgtcttc gagggagagg
    16141 aagaagcgcg aagcgcccgg atcgccctgc cggcccgaac ggccgcggag ctgattgtcg
    16201 atgcggcgcg actcgtggcg ctcggtgccg atgatttgaa ggccgcccgc gtctttgacg
    16261 cgcttggcgt cttctttgca tttgctgatg tagagctcgg tgttcttggc gatggcggct
    16321 tcacgacgct ccggatcgga gagcacgtcc tctggggccg tctccaacac ctcgatccgc
    16381 gcgagcatct cggcgttgcc gccaagcacg atgtcggtgc ctcgacccgc catgttggta
    16441 gccaccgtca ccgcgccggg cgtgcccgcc tgcgcgacga tgtaagcctc gcgctcgtgc
    16501 tgtttcgcat tgagcacgtg atgtggaacc ccctgacggg tgagaaagtg cgcaaccgca
    16561 tcggacttct cgacactcgt cgtacccacg agcacgggct gaccccgctt gtaggactcc
    16621 tcgatctctt ggcagacggc gcggaacttc tcgcgctcgg ttttgtagat caggtcttct
    16681 tcatcgatac gggcgatcgg tttgttggtc gggatgacca cgacatcgag cttgtagatg
    16741 ttgtggaact cggtggcttc ggtgtcggcc gttccggtca tgccggagag cttcttgtag
    16801 agacggaaca gattctggaa cgagatcgtc gcgagcgtga ggttctcact ctgaatgaga
    16861 acgcgctcct tggcctcgac cgcttggtga agcccgtccg accagcggcg accagctagc
    16921 acgcggccgg tgaactcgtc gatgatcatc accttgccgt cctcggtcac catgtagtgc
    16981 tgatcacggt ggtagagtgc gtgcgcccgc aagcactgct gcaagatgtg aagggtctcg
    17041 aggttggccg ggtcgtagag gttgccgtcg ccgccgagga gcccacgtcc gccgaggagc
    17101 tcctgtgcgc gctccatgcc ttcttcggtg agtgtgacgc tgtggttctt ctcgtcgagg
    17161 tcgaagtgct cctccttgcg gaggcgcggg atgacctcgt tgatcatctc gtacttctcg
    17221 ctggcggtct cgcccggacc gctgatgatc aggggcgttc gcgcctcgtc gatcagaatg
    17281 gagtcgacct catccacgat cgcgtagttc agctcacgct gcgcgtagtc gtaaatgctg
    17341 aacttcatgt tgtcgcgcat gtagtcgaac ccgaactcgt tgttctgtcc gtacgtgatg
    17401 tcgcaattgt acgcgcgctt cttgagttgg ttgctttgac ccggcaccac gacgccggtg
    17461 ctcaatccga ggaaaccgta gagcttgccc atccattcgg cgtcgcgcga tgcgaggtag
    17521 tcgttcacgg tgacgacgtg cacgcccttg ccctcgagtc cgttcaggta gcagggaagg
    17581 gtcgcgacga gcgtcttgcc ctcacccgtc ttcatctcgg cgatcttccc ctggtgaaga
    17641 acgatcccgc cgacgagctg aacgtcgtag tggcgcattc cgagcacgcg cttgcccgct
    17701 tcacgggcca cggcaaaggc agggaccagc agctcgtcca cagtcgcacc gttgtcgagc
    17761 tgttgccgaa actccgcggt cttgccccgt agttgcgcgt cggagagctt cttcgtttcg
    17821 tcttcgagag ccgaaatcgc cgccaccatg ggctgcatgc gcttcacttc gcgagcgtgc
    17881 ttggtgccga acaccttctt gagagccgag ttgaacacgg ctgcaatcta ggtcggccgg
    17941 gggagccgcg caaacgacag ttaaaaccaa cgcttggaga gcgcgagcag cgccatcaag
    18001 acgaagacca ggagaacgaa cgcccacaag ccccgacccg atgacgaccc gcgggacggc
    18061 gatgtgaacc catccccgag ctcgtcgaga agctgccggg cacgggcctg cgcaagcagc
    18121 tcgacgcgca acaagaccat gctcaccatg agaccgatga gcagaatcac cgcggcagcc
    18181 tgccaccgcc tcagctcctg ctcgctttcg agggttccat gggcgccgac gcgcgcgctc
    18241 gccccggcgc cggtcgaaac gacgtcgaag ctcggaaccg caaagagcgc acgctcgagc
    18301 gtctgctcat cctcgacctg gagcgttccc tcgatcactg cgaccgcgat cggatcgaga
    18361 ccttcgcgat cggcctctgc gaggaccgca tcggcagctt gccgcgcccg gccgggcccg
    18421 ccgggatccg cgatgaccag gtactggacg gctgccgtat tgtccgagaa gagatcggaa
    18481 cgaacctgaa gacgccagac accgggtcca aacgctggca gataggggtg ctcgggagca
    18541 agggagaagg ctcgaaccca tcgatgtccc tgaaacgcgt cgaccaagac gggaccgcgt
    18601 gccccgagtt gctcccagtc gagacggatt cgttccttct cttccgagat ccgaacgacg
    18661 atgccccctg gcaccagcgg aagtcgcgcc tcgcgtgccg caattaggtc gccatcctcc
    18721 gagaatagct cgacttcgac gcgcgcttcg gtgccgccca cgagcaatgg aatccgcacg
    18781 aacccatccg tcgaagcgaa ggtgccctgc tccaggcgga cgcctgcgag cgggcgtacg
    18841 cgtactcgcc cctcctcgtc agcgacccaa accaggagcc agcagcgcag gtcgggaacg
    18901 caagcacctt cctcgacacg gggatcgagc gccgcgatcg aacggcggga gctcgcgaga
    18961 cgaatcggcc cgagctcgta tacctggaaa gcgttgactt cccgtcccct cggaaggcga
    19021 gattcgatcc cctcctgcac atagagggcg cgctcgaccg agacgtcgcg tccgtcgatt
    19081 tctgcgtgtg cgaccaaggt cagcacctcg tcgacccccg cgggaatact cagccgacct
    19141 tcacgtccct gcacgaggga ttccacgagc tctgtcttcg caaggacgag gccgacttcg
    19201 ttctggagct cgaccagcac gttaggtgcc aggatgaccg gatatccgtt ctcgtcttcg
    19261 gccagttgcc aggcacggag cccaatgctg gttccgggtt gcgcgacgag cggcgcactc
    19321 aaacgaagat cgggaacctc aggaggggtc cccgcgatcc agagcgttgc gaggataatc
    19381 gcgacgagca acggcgacat tcggatcgcc caggcgaatc cgttgaattg tcgttcctgt
    19441 cgggtgatgg ggatggacaa g
//
LOCUS       DASRZF010000068        12565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3835362, whole genome shotgun sequence.
ACCESSION   DASRZF010000068 DASRZF010000000
VERSION     DASRZF010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 12565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12565
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3835362"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="VFG22_03030"
     CDS             complement(<1..580)
                     /locus_tag="VFG22_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6415245.1"
                     /translation="MIEVEGLSRRYGSRLAVSDVSFRVQPGEIVGFLGPNGAGKTTTL
                     RMLTGYLAPSSGTIRIDGIDAVDDSIRARQRLGYMPEGVPLYGEMRVHEYLRHRAALK
                     SVSSLSEAVDRSLERAGVADARTRIIGQLSKGYRQRVGLAEALIADLAILILDEPTSG
                     LDPNQVRHFRELVRSFQGKKTVLLSTHILSEVE"
     gene            700..1680
                     /locus_tag="VFG22_03035"
     CDS             700..1680
                     /locus_tag="VFG22_03035"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET6415246.1"
                     /translation="MPWIPWKVRLSCCLLALVAGGSLGGPSEMRPGPIAIAPITIRPS
                     PIREPRHGEPGPEVPGLVPIVSDDVTGLRFPSGLTIYGGTRRRSILFTFDDGPSRRTT
                     PALLDILDDLDIKAVFFVKSESFGNGNPWEREHAEIVREIVRRGHLVGNHTETHRQLP
                     LLSNAEIDAELAVSERKIEWTLGRTPRLIRPPGGALSQRVEQLLAERGYSSVMWALYA
                     GDLEVETPDDVVRTFFRVLDRRELDTGDRGGIVLMHDTKRHSVEALPRLVEALRRRNC
                     KLLAKDEELYDIVDDLGYFIPGHEPDQTLDERQAEIRDRARRSCHTVAVR"
     gene            complement(1681..2298)
                     /gene="trmB"
                     /locus_tag="VFG22_03040"
     CDS             complement(1681..2298)
                     /gene="trmB"
                     /locus_tag="VFG22_03040"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HET6415247.1"
                     /translation="MSDSDLRRLYREGAPRAKEGRFSLQDLIAGSGELELDIGFGRGL
                     SLFERAVAAPQNRIVGIEVKTKLAFLVNERLRKREIHNVAVLCGDAREILKRAEPDGS
                     VRRVALHFPDPWWKKRHDKRRVIGEALIAELSRLMMPGAELFIQTDVEHRAEQYVAQL
                     REAPTFSLASGSGYVDENPFDARSNREKRAIEDGLPVWRILATRS"
     gene            2412..3230
                     /locus_tag="VFG22_03045"
     CDS             2412..3230
                     /locus_tag="VFG22_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6415248.1"
                     /translation="MSMRILIVEDSVTMRKVMEMTFAGEDAELLAVESGELALQKGRD
                     FGPDVAFIDASLPGLDGYEVTRAIKGDPQLAGTAVILMASQHRPLDDARASEVGADDH
                     ILKPFDTQEAINKAVDLASRAEELYEAPSIPVARPATTQPRPISQPSYRPTPPPLPIN
                     GPSARPMAPSPYAATPLTGAQVSVAPGMVSSYPAPSPPRRAASSAPQPASAAVGKAIS
                     HSVVERLQTLGLTPDQVEGVLKLSAEVVERVVWEVVPELAEAMIREELHRLVGD"
     gene            3272..6055
                     /locus_tag="VFG22_03050"
     CDS             3272..6055
                     /locus_tag="VFG22_03050"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HET6415249.1"
                     /translation="MSEPSDEMPTQYSPTESEARWYEFWETSGFFAASEDPADDRETY
                     TLAIPPPNVTGSLHMGHACRTTFEDVLCRYKRMQGYNTLWVPGIDHAGIATQVVVERQ
                     LKKEGLTRHDLGREKFIERVWQWKEESGGRILQQLRKMGASCDWARTKFTMDPDMVRA
                     VREAFVRLYEEGLIYRDTRLVNWDVESQTVLSDLEVEQEENVEGAMFDFAYPLADGDG
                     EVVVSTTRPETMLGDTAVAIHPDDPRYRHLHGKYVRHPFVDRLIPVILDAELVDPEFG
                     TGVVKVTPAHDPNDFATGKRHGLEEINIFNLDGTMNENAGPFQGLERFQARQAVEIEL
                     GNRGLSRGTKKHMMTLPRSQRSGTIVEPMISTQWFVQMKPLAEPAIKAVEDGSIQILP
                     PEWSKTYFHWLRNIQDWCISRQLWWGHPIPAWYCADCEHVTVSREDATVCGGCGSASI
                     RQDEDVLDTWFSSGLWPFSTLGWPDKTASLERFYPTQDMETGYDILFFWVARMIMMGL
                     HFMGDVPFTRVLLAGLVTDERGEKMSKVKGNVVDPLDVIYGATLEQLLAKAEKAGAKK
                     SGLKYLKSTYPDGFSAYGADALRMTLLSYSPQSRRIALSIKRIEGYRNFANKLWNASR
                     YALMRLSGSSTTATQTRPQPRALANRWVLSRLYHALDVASSGLDAYRLDEASGALYHF
                     VWGEFCDWYLELSKPALDSGDAALVQETEATLVHVLETVLRALHPMMPHITEEIWQRV
                     PKWNDAPASIMISRFPTGLVDAKLDRNAERDFAVVQAFVSSARTIRAEHDVPKSQPMP
                     IHWHSDDESKRAVLEAERATVEALARCEMRFEADPAKLDDASAHFENAAVFVNPGVRA
                     AVPGVIDTEKERDRLRRDLKKIEKELVFIEQKLSNPSFVERAPEAVVEKSKRDASQLR
                     EKRERLEAALNSL"
     gene            6066..7526
                     /locus_tag="VFG22_03055"
     CDS             6066..7526
                     /locus_tag="VFG22_03055"
                     /inference="COORDINATES: protein motif:HMM:NF006088.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HET6415250.1"
                     /translation="MNTPGTDPSASASKGPLCVDLDGTLVAADTTAESLFELLKTAPW
                     KIAALLVAWMGGRPKLKRYLAQTAPVDPASLPYRDEVLNAMGEAREEGRRILLVTGSD
                     QATADAVAAHLGQFDEAIGSDGKTNLKAHRKAELLVSRFGKGGFRYLGDSRADLPVWN
                     AANEALMVCPSLRTQRAAERSLSKTVVLVERPKKLAAAVRELRPHQWAKNILLFVPLY
                     FSHLYSDLSLVFTAVMAFFAFSFCASSIYVLNDLVDLPSDRLHPRKRKRPLAAGTLSI
                     PEGMALSLAALALSMTLAVVCVNQRFVLVLLGYVALTTAYSFVLKQKMIVDVLTLASL
                     FTYRVMAGGVAVDVKLSPWLLAFSIFFFTSLAFVKRYSELIQVKGDGGDSLKGRNYVP
                     ADIPIISSAGPAAGLLAVLVFALYINSPVMLRYYSVPEALWGICLILVYWIIRIWFLA
                     ARGRMHDDPVLFAVKDRVSLAAGALVVLCLLVARYG"
     gene            complement(7528..8076)
                     /locus_tag="VFG22_03060"
     CDS             complement(7528..8076)
                     /locus_tag="VFG22_03060"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HET6415251.1"
                     /translation="MTAERISENKPPAAPVLGWGTLEAKGLGTLWLEWSAAGLLRLSF
                     EPLDDTLADECSVPEMFNKPLGRYFDAEPEDFSAVPLDLRGTEFQVRVWNALREIPWG
                     QVRTYGGIANTIQSPRAMRAVGQANHVNPIPIIVPCHRVVEAGHHLGGYGGGLERKQL
                     LLELEGVRLHEGVVQPGQMELF"
     gene            8175..10280
                     /locus_tag="VFG22_03065"
     CDS             8175..10280
                     /locus_tag="VFG22_03065"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HET6415252.1"
                     /translation="MGPFDDGVVRFVPEEVDPSTLPRSFALTAPVSALNGSSDPSRPI
                     PKFGHGAGGYSATVPIDPGTSLYGTGEVAGPLLRNGFVTECWATQPFRIEDNGEVVAN
                     YDEHSKSLYQAHPWVLGVSADGSAFGVLADTTYRLEIDLRNGIRLSSAKPFAVIMIEG
                     ASPDEVVERLATLTGRIELPPHWALGYQQCRFSYYPDARVREIASELRKRSIPCDVIW
                     VDIHYMDNYKVFTFDPDRFPDPKGTNQFLHGLGFKSVWILDPAVKVEPGYSVYDEGVS
                     DRHFLLDANGQEHHESTWPGEAAFPDFTRPETLAWWTRLTVAFLEQDIDGLWVDLNEP
                     SPILPLGAELPESLMHRGGGEIAPGTHAQYHNVYGLLMSKATHDAMRIANPERRPFVL
                     SRSSYLGGQRYAATWTGDNVSSWQHLLWSVPMVLNLGLSGQPFSGPDIGGFAGTPSPE
                     LFAHWIGVGAFLPFCRTHNSLTGDQEPWSFGPAVEEIARTALACRYRLLPYLYTAFRD
                     AATRGLPVARPLFFADPADAALRAEDHAFLLGGDLLVQPQLMNGPGRDFATPKGRWRT
                     LSLLGGDAGGESAHPRLRLRDGAILPVGQGGQTAAEAFSGPLTLFVSLDESGRARGEL
                     YEDAGEGFGYLDGDYLLTTYQARRIGAAVEISVARQEGDRPREARELSVELLTDQGQR
                     RAHGRDGERIRIPFDASAP"
     gene            complement(10277..>12565)
                     /locus_tag="VFG22_03070"
     CDS             complement(10277..>12565)
                     /locus_tag="VFG22_03070"
                     /EC_number="2.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016157 - sucrose synthase activity
                     [Evidence IEA];
                     GO_process: GO:0005985 - sucrose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose synthase"
                     /protein_id="HET6415253.1"
                     /translation="HEFEDFSASDVGASLLETKMSWIMRHTQEAVVDEPFVCLALRAR
                     VGRWHYVQVETEDMRCRELTVNEFLDIKERIAAGIPHGERYVLEIDLSPFERRFPKLK
                     DPRSIGRGVEFLNRHLAVRLFTDGGSGQKLLFDFLQVHQVRGQQLMLNGTLRDVDELR
                     EALAEATALLEGSESETNGFNRELRRLGFEPGWGRAPDRIRETMELLLDILEAPSPQN
                     LESFLERIPMIFSIAIISPHGYFGQSNVLGKPDTGGQVVYILDQVRALEKEMRDSIYE
                     QGLDIDPQIVVLSRLIPESDGTTCDQRIEPILGTENARILRVPFRTYHGGEIISKWIS
                     RFEVWPYLERYAIDAERELLAELGGVRPDIVIGNYSDGNLVASVLAHRFGVTQCNIAH
                     ALEKTKYLLSDLHWQNHEADHHFAAQYTADLMAMNTADFIITSTYQEIAGTDESVGQY
                     ESYGSFSLPRLYRVISGIDCFDPKFNIVSPGADPRIFFPYFEEARRPQELRDEIRNMV
                     YGSPECAFRGLLTDQDKPLLFAMSRLDRIKNMAGLVEWYAQNDELRDMANLLIVGGRL
                     RVEESSDRDERQQIERTHWLFDEYGLEGNVRWVEMQTDKTKVGGLYRFVADQQGAFVQ
                     PALFEAFGLTVVESMSTGLPTFATRYGGPLEIIEDGVSGFHIDPTQGEETAKKMLEFF
                     RACREDPSLWNAVSRAGIRRVDERYNWPLYASTLLKLSRVYGFWRYISSLERDETRRY
                     LEMFYGLLLRPLSKRVLEQTQH"
BASE COUNT         2327 a         3907 c         3903 g         2428 t
ORIGIN      
        1 cttctacttc ggaaagaatg tgcgtagaga gcagaacggt tttcttgcct tggaagctgc
       61 gcacgagctc gcggaagtgt cggacctggt tggggtcgag gcccgaggtc ggctcgtcta
      121 ggatgagaat ggctaggtcg gcgatcaggg cttcggcgag acccactcgt tgccggtatc
      181 ctttcgagag ctgaccgatg attcgtgtgc gcgcgtcggc aacccccgcg cgctcgagag
      241 agcggtctac ggcttcggaa aggctggaga ccgacttgag cgcagcacga tggcgcaggt
      301 actcgtgcac cctcatttcc ccgtagaggg gtacgccctc cggcatgtac ccgagccgct
      361 gccgggctcg aatcgagtcg tcgacggcat cgatgccgtc gatccgtatt gttccggagc
      421 tcggcgcgag atagccggtg agcatcctga gggtcgtggt tttcccggcc ccgttcgggc
      481 cgaggaatcc tacgatttca ccaggttgga cgcggaacga aacatcgctt accgcgagcc
      541 tgctcccata ccgcctactg agaccttcga cttcgatcac tgattgctcg ttgggcgcac
      601 ggggtactcg atgcgatccc cgcttgcaac tgcagacctg tccagttgct gcggctttct
      661 cgctttgctt gccccggctt ccatacgatc ggcggcgaga tgccgtggat tccatggaag
      721 gttcgcctca gctgctgcct ccttgcgctc gtggccggtg gctcgctcgg cggcccttcc
      781 gagatgcggc ccggtccaat tgcgatcgcg ccgatcacaa tccgcccttc cccgatccgc
      841 gagcccaggc atggggagcc cgggccagag gtgcctgggc tcgttcctat cgtcagcgac
      901 gacgtgactg ggcttcggtt cccgagcggc ctcacgatct acggcggcac gcgccggcgc
      961 tcgattctgt tcaccttcga cgatgggcct tcccgccgga cgactcccgc gctcttggac
     1021 atcctcgatg atctcgacat caaggctgtc ttcttcgtga agagtgagag ttttggcaac
     1081 ggcaaccctt gggaaaggga gcacgcggag atcgtgcgcg agatcgttcg tcggggacat
     1141 ctcgtcggga accacaccga aacccaccgg cagctgccgc tgctatcgaa cgccgagatc
     1201 gacgcggagc tcgccgtgag cgagcggaag atcgagtgga cactcgggcg aactccccgc
     1261 ctcattcgac cgcctggcgg agcactctcc cagcgggtcg agcagctcct tgctgagcgc
     1321 ggatacagca gcgtgatgtg ggcgctatac gcaggggatc tagaggttga aaccccggac
     1381 gatgtggtcc ggaccttttt ccgggtcctc gatcgccgcg agctggatac tggggatcgc
     1441 ggcggcatcg ttttgatgca cgacaccaag cggcatagcg tcgaagcgct gccgcgcctc
     1501 gtcgaagcac tccggaggcg caactgcaag ctgctcgcga aggatgaaga gctctatgac
     1561 atcgtcgatg acctgggtta cttcattccg gggcacgaac ccgatcaaac gctcgatgag
     1621 cggcaggcgg agattcggga tcgagctcgt cgctcgtgcc acacggttgc ggtccggtag
     1681 ctagcttcgc gtcgcgagaa tccgccaaac cggcaggccg tcctcgatcg cgcgcttctc
     1741 gcggttcgac cgagcgtcga aggggttctc atcgacatag ccgcttcccg aagcgagcga
     1801 gaacgtgggc gcctcgcgaa gctgcgcaac atactgctcg gcacgatgct ccacgtcggt
     1861 ctggatgaag agctcggccc caggcatcat gagacgggag agctccgcga tgagtgcctc
     1921 tccgatgacc cggcgcttgt cgtgccgctt cttccaccat gggtctggga agtggagcgc
     1981 cacgcgccga accgatccgt ccggttcggc tcttttcaag atttcgcgcg catctccgca
     2041 aaggacggcg acattgtgaa tctcgcgctt ccggagccgt tcgttgacca ggaatgccag
     2101 cttggtcttg acctcgatgc ccacgatgcg gttttgcgga gcggccaccg cccgctcgaa
     2161 aagcgacaaa ccacggccaa aaccgatgtc cagctccagc tcacctgatc ccgcgatcag
     2221 gtcctgcagc gagaagcggc cttctttggc ccggggagcg ccttccctgt aaagccggcg
     2281 taggtccgaa tccgacaccg tccgtcccta cggttgcccg cggaacgggt caaaccggat
     2341 acgctccttc ttgaccggca aggcaccgat cgcgtatgcc aacgagcata catctaggtg
     2401 aatggggctt gatgtcgatg aggattttga tcgtcgaaga cagcgtgacc atgcgcaagg
     2461 tcatggaaat gactttcgcg ggggaagacg cggaattgct tgccgtcgag agcggtgagc
     2521 tcgcacttca aaaagggcgt gactttggcc cggacgtcgc attcatcgat gcgtcgctgc
     2581 ctggtctgga cgggtacgaa gtgacacgcg caatcaaggg cgacccgcaa ctcgctggta
     2641 cggccgtgat cttgatggcg agccagcatc gcccgctgga cgacgcgcgc gcatccgaag
     2701 tcggcgcgga cgatcacatc ttgaagccat tcgatacgca ggaagcgatc aacaaggccg
     2761 tggatctcgc cagtcgtgcc gaggagctct acgaggcacc ctcgatcccc gtcgctcgtc
     2821 ctgcgacgac gcagccccgg ccgatttctc agccctctta ccgccctacg ccgccgccat
     2881 tgccgatcaa cggcccgtcc gcacggccca tggcccctag cccgtacgct gcgacgcctc
     2941 tcactggtgc gcaggtttcg gtcgctccgg gaatggtctc ctcgtatccg gcgccttcgc
     3001 cgcccaggcg ggccgcatcg tccgcccctc agccggcctc tgccgcggtc ggcaaagcga
     3061 tctcacattc ggtcgtggag cggcttcaga ccctcggcct cactcccgat caagtcgaag
     3121 gcgtgctcaa gctctcggcc gaggtcgtcg agcgcgtggt gtgggaggtc gtaccggagc
     3181 tcgccgaagc gatgatccgt gaggaattgc accgtctcgt cggtgactga gacttgactg
     3241 cccagcgatt tcaccgcaca cggtcggtcg gatgagcgaa ccgtctgacg agatgcccac
     3301 gcagtactcg ccgaccgaga gcgaggctcg ctggtacgag ttctgggaga cctcgggctt
     3361 tttcgcggcc tcggaagacc ccgccgatga tcgggagacc tacaccctcg cgatcccccc
     3421 tccaaacgtc accggctcgc tccacatggg gcatgcgtgc aggacgacgt tcgaggacgt
     3481 actgtgccgc tacaagcgga tgcagggcta caacaccctc tgggtgcccg ggatcgacca
     3541 tgccgggatc gccacgcagg tggtcgtcga acgccagctc aaaaaggaag ggctcactcg
     3601 gcacgacctc ggtcgcgaga agttcatcga gcgggtttgg cagtggaaag aggagtccgg
     3661 cgggcggatt ctgcagcagc tccgcaagat gggcgcgtcc tgcgattggg cgcggacgaa
     3721 gttcacgatg gacccggaca tggtgcgggc ggttcgtgag gcgttcgtac ggctctacga
     3781 agaggggctg atctaccgcg acacgcgcct ggtcaactgg gacgtcgagt cacagaccgt
     3841 gctttccgat ctagaggtcg agcaggaaga gaacgtcgaa ggcgcgatgt tcgatttcgc
     3901 ctatccattg gccgacgggg acggcgaggt ggtcgtttcg acgacgcgcc cagagacgat
     3961 gctcggcgat accgccgtcg ccatccaccc cgatgaccca cggtatcgcc atctgcacgg
     4021 gaagtacgtg cggcatccgt tcgtcgaccg gttgattccg gtaattctcg acgcagagct
     4081 ggtcgacccc gagttcggca ccggagtcgt caaagtgacg ccagcgcacg acccaaacga
     4141 ctttgcaacg ggcaaacgac acggcctcga agagatcaac atcttcaacc tcgacggcac
     4201 gatgaacgag aatgcgggcc ccttccaagg gctcgagcga ttccaagcgc gtcaggcggt
     4261 cgagatcgag cttggcaacc gtgggctttc ccgcgggacc aagaaacaca tgatgacgct
     4321 gccccggtcg cagcgttccg gcacgatcgt cgagccgatg atctcgaccc agtggttcgt
     4381 gcagatgaag ccccttgctg aaccggcgat caaggccgtc gaggacggct cgattcaaat
     4441 tcttccgccc gagtggagca agacgtattt ccactggctt cgcaacatcc aggactggtg
     4501 catctcccgt cagctctggt gggggcaccc gatcccggct tggtactgtg ctgattgcga
     4561 gcatgtcacc gtctcgcgcg aagacgcgac ggtctgcgga ggctgcggca gcgcctccat
     4621 tcgacaggac gaggacgttc tcgatacctg gttcagctct gggctctggc ccttctccac
     4681 actcggctgg cccgacaaga ccgcgagcct cgagcgtttc tacccgacgc aggacatgga
     4741 gacggggtac gacatcctgt tcttctgggt cgcccgcatg atcatgatgg gccttcactt
     4801 catgggcgac gttcccttca cgcgtgtctt gctagcgggc ctcgtgaccg acgagcgcgg
     4861 cgaaaagatg agcaaagtga aaggcaacgt ggtcgatccg ctcgacgtga tctacggcgc
     4921 gacgctcgaa caactgcttg caaaggcgga gaaggcaggg gccaagaaat ccggcctcaa
     4981 gtacctgaag agcacgtatc cagacgggtt ctccgcgtac ggcgctgacg cgctgcgaat
     5041 gactctcctc agctattccc cccagtcgcg acgcattgcc ctctcgatca aacggatcga
     5101 aggctatcgc aacttcgcca acaagctttg gaacgcgtcg cgttatgcgc tcatgcgcct
     5161 gagcggctcc tccacaacgg ctacgcagac gcgcccccag cccagggctc tcgccaaccg
     5221 ctgggttctg tcgcggctct accacgcgct cgacgttgcc tcgtccggat tggacgcgta
     5281 ccgcctcgac gaagcatctg gcgctctcta tcacttcgtc tggggcgagt tctgcgattg
     5341 gtatctggag ctgagcaagc cggcgctcga cagcggtgac gcggcgctcg ttcaagagac
     5401 cgaggcgacc ttggtgcacg tgctcgaaac ggtgctgcgt gcccttcacc ccatgatgcc
     5461 gcacatcacc gaggagatct ggcagcgggt gcccaagtgg aacgacgcgc cggcaagcat
     5521 catgatcagt cggttcccaa cgggattggt cgatgccaag ctcgatcgca acgcagaacg
     5581 cgacttcgcc gtggttcagg ccttcgtcag cagcgcgcgg acgatccgcg cagaacacga
     5641 cgtgccgaag agccagccga tgccgatcca ctggcacagc gacgatgaga gcaagcgagc
     5701 cgttctcgag gcggagcgcg cgacggtcga agcgttggca cgttgcgaaa tgcgcttcga
     5761 ggcagaccca gccaagctcg acgatgcgag cgcccacttc gagaacgctg ccgttttcgt
     5821 caatcctgga gtccgggcgg ccgtgcccgg tgtcatcgat accgaaaaag aacgagatcg
     5881 gcttcggcgc gacctcaaga agatcgaaaa ggagctcgtt ttcatcgagc agaagctcag
     5941 caatccgagc ttcgtcgaac gcgcgcccga agccgtggta gaaaagtcca agcgcgatgc
     6001 gtcgcagctg cgcgagaagc gcgagcgatt ggaagctgcg ttgaatagcc tttgagaact
     6061 ggactttgaa cacgcccggc acagatccaa gcgctagtgc aagcaagggg ccactctgcg
     6121 tggacctcga tggtactctc gtcgccgcgg acacgacggc cgaaagcctg ttcgagctcc
     6181 tcaaaacggc tccttggaag atcgccgcgc tcctcgtggc ctggatgggt ggccggccga
     6241 agctcaagcg ttacctggcg caaaccgcgc ccgtcgatcc cgcttcattg ccctaccggg
     6301 acgaggttct caacgcgatg ggcgaggccc gcgaagaagg tcgaagaatt ctgctggtga
     6361 ccggttccga tcaagccacc gcggatgctg tggcggcgca tctggggcag ttcgatgagg
     6421 cgatcggcag cgacggcaaa accaacttga aggcgcatcg aaaggcggag ctgctggtct
     6481 cgcgatttgg caaaggaggg tttcggtacc tgggtgattc acgggccgac ctgcccgtat
     6541 ggaacgcagc gaacgaagcg ctcatggtat gcccgagcct gcgcacgcag agggcggcgg
     6601 agcggagcct ctccaagacc gtggtcctgg tcgagcgtcc aaagaagctg gctgcggcgg
     6661 tccgggagct ccgcccgcac caatgggcga agaacattct gctcttcgtg ccgctctact
     6721 tttctcacct ctacagcgac ttgagcctgg tgttcacggc ggtgatggcg ttcttcgcgt
     6781 tcagcttctg cgcttcgtcg atatacgtct tgaatgatct ggtcgatttg ccatcggatc
     6841 gcctgcatcc gcgcaagcgc aagcgcccgc ttgcggcggg aaccttgtcg atcccggaag
     6901 gcatggcgct ctcacttgcg gccctcgcgc tgtccatgac tctagccgtc gtgtgcgtga
     6961 accagagatt cgtcctggtg cttctcggct acgtcgcgct aaccacggcg tactcgttcg
     7021 ttctcaagca gaagatgatc gtcgacgtgc tcaccctcgc ctcgctcttc acctaccgcg
     7081 tgatggcggg cggcgttgcg gtcgacgtga agctttcgcc ttggcttctt gcgttttcga
     7141 tcttcttctt cacgagcttg gcattcgtga aaaggtactc cgagctgatt caagtgaagg
     7201 gtgatggtgg cgactcactc aaagggcgca actacgtccc ggcggacatt ccaatcatca
     7261 gctcggccgg gccagcggcg ggactgctcg cggtcttggt cttcgctctc tacatcaaca
     7321 gcccggtaat gttgcgatac tactcggttc cggaggccct ttggggcatc tgtctcatcc
     7381 tggtgtactg gatcattcgc atctggtttc tcgccgcacg cggtcgcatg cacgatgacc
     7441 ccgtgctgtt cgcggtgaaa gacagggtca gccttgccgc tggtgccctc gtcgtgctgt
     7501 gccttctggt cgcgcgctac ggctagctca gaagagctcc atttggccgg gctggaccac
     7561 gccttcgtga agccgaacgc cttcgagctc gagcaagagc tgcttgcgct cgagcccgcc
     7621 accgtagccg ccgagatgat gcccagcttc gacgacgcga tggcatggaa cgatgatcgg
     7681 gatgggattg acgtggttcg cttggccgac cgcacgcatg gcacgcgggg actgaatcgt
     7741 gttcgcgatg cctccgtagg ttcggacctg gccccaggga atttcgcgca gtgcgttcca
     7801 gacgcgaact tgaaactcgg ttcccctcag atcgagcggg accgcggaga agtcctctgg
     7861 ctcggcgtcg aaatagcggc ccagcggctt gttgaacatc tccggaacag agcactcatc
     7921 ggcaagggta tcgtccaaag gctcgaacga gaggcgcagc aagccggcgg cgctccattc
     7981 gagccagagc gtccctaggc ccttcgcctc cagggttccc caacccagga ccggagcggc
     8041 cgggggcttg ttctcgctga tgcgttccgc tgtcatggct cgctgcttcg ttggatcgat
     8101 tctcgtactc tcaattgcat gtgaacgacc gcttaaacaa gcgcaaatac gattggaacg
     8161 ctagggtctg acacatgggg ccattcgacg atggggtcgt gcgtttcgtt cccgaggagg
     8221 tcgacccttc gacactcccg cgctctttcg ctctcaccgc gccggtttcg gctctcaatg
     8281 gctcatctga cccatctcgc cccattccga agttcggaca tggtgcgggg ggatacagcg
     8341 cgaccgttcc tatcgatcct ggaacgagcc tatacggaac cggcgaagtc gcggggcctc
     8401 tgctccgcaa tggattcgtc accgagtgtt gggccacgca gccctttcgc atcgaggaca
     8461 acggcgaggt ggtcgcgaac tacgatgagc actccaagag cctctatcag gcgcatccct
     8521 gggtgttggg ggtgagcgcg gacgggagcg cgtttggcgt gctggccgat acgacctacc
     8581 gactggaaat cgatcttcgc aacgggatac ggctgagctc ggcaaaaccg ttcgcagtaa
     8641 tcatgatcga aggggcatcc ccagacgaag tcgtggagcg attggccacc ctcacggggc
     8701 ggatcgagct tccgccacac tgggcgcttg gctatcaaca gtgtcgcttc tcctactatc
     8761 ccgatgctcg agtccgggaa atcgccagtg agctccggaa gcggtcgatc ccttgcgacg
     8821 tgatctgggt cgatatccac tacatggaca actacaaggt gttcacattc gacccggatc
     8881 gcttccccga ccccaagggc acgaatcagt tcctgcacgg cttgggcttc aagtcggtgt
     8941 ggattctcga tcccgccgtc aaagtggaac ccggctactc ggtatacgac gaaggcgtct
     9001 cggaccggca tttcctcctc gacgcgaacg ggcaagagca ccacgagagc acctggccgg
     9061 gcgaagctgc atttccggac ttcacgcggc cggagacgct ggcttggtgg acccggctca
     9121 ccgtcgcctt cctggagcag gatatcgacg gactctgggt cgatctcaac gagccctctc
     9181 ctattttacc gcttggcgcg gagctgcctg agtccttgat gcatcgtggc ggaggcgaaa
     9241 tagcgcccgg aacccatgct caataccaca acgtttacgg gctcttgatg tccaaagcca
     9301 cacacgacgc gatgcgaatt gcgaatccgg agcgccgtcc attcgtcctc tcccgctcga
     9361 gctatctcgg tggtcaacgc tacgcggcga cctggaccgg cgacaacgtc tccagctggc
     9421 agcacctttt gtggtcggtg cccatggtct tgaatctcgg cctctcaggg cagccgttct
     9481 ccgggccgga cattggcggt ttcgcaggca cgcccagtcc tgagctcttc gcgcattgga
     9541 tcggcgtcgg agctttcctc ccgttctgtc gcactcacaa ctccctaacg ggcgatcagg
     9601 agccctggtc attcgggccg gcggtagaag agatcgcacg gacggccctt gcatgccgct
     9661 accgacttct tccctatctc tacacggcct tccgcgacgc cgcgacgcgc ggactccctg
     9721 tcgcgcgtcc tttgtttttc gcggatccag cggatgccgc tttgcgggcc gaggatcacg
     9781 catttctgct gggaggcgac ctgctggtcc agccccagtt gatgaacggg ccgggccgtg
     9841 atttcgcaac accgaaaggc cgctggcgca cgctgtcctt gcttggagga gacgctggcg
     9901 gcgaaagcgc ccatcctagg ctccgcttgc gcgacggcgc gatccttccc gtcggccagg
     9961 gtgggcagac cgccgcggag gcattctcag gcccgctgac cctgttcgtc tcgctcgacg
    10021 aatccggcag ggcccgcgga gagctctatg aagacgctgg cgaaggcttt ggctacctcg
    10081 atggtgacta tcttctcacg acgtaccagg cacggcgaat cggtgcggca gtagagattt
    10141 cggtcgccag gcaagagggt gacaggccga gggaggcccg cgagctcagc gtcgagcttc
    10201 tcaccgacca aggacagcgc cgcgctcacg gacgcgatgg cgagcgcatt cggatcccct
    10261 tcgatgcctc agcgccctag tgctgggtct gttcgagcac ccgcttcgac aagggacgca
    10321 agagcaggcc gtagaacatc tctagatagc gtcgggtctc atcgcgctcg agcgaagaga
    10381 tatagcgcca gaagccgtag acacgagaga gcttgagcag cgtcgaggcg tagagtggcc
    10441 agttgtaccg ctcgtccacc ctgcgaatgc cagcccgcga aaccgcgttc cagaggctcg
    10501 ggtcttcacg acaggcgcga aaaaactcga gcatcttctt cgcggtttcc tcgccttgag
    10561 tgggatcgat gtggaatccg gagacgccgt cctcgatgat ctcgaggggg ccgccgtacc
    10621 gagtggcgaa agtgggcaac ccggtgctca tcgactcgac gaccgtgagg ccaaacgctt
    10681 cgaagagggc gggctgaacg aaggcgccct gctgatcggc gacgaagcgg tagagacccc
    10741 ccaccttggt tttgtcggtc tgcatctcga cccatcggac gttcccctcg agtccgtatt
    10801 cgtcgaacag ccaatgagtg cgctcgatct gttggcgctc gtcgcggtcg cttgactcct
    10861 cgacgcgaag tcggcctcct acgatcagga ggttcgccat atcgcgcagc tcgtcgtttt
    10921 gcgcgtacca ttcgaccagg cccgccatgt tcttgatccg gtcgaggcgt gacatcgcaa
    10981 agagaagcgg cttgtcttgg tcggtcagca ggcctcggaa agcgcactcg ggcgagccgt
    11041 agaccatgtt tcttatttcg tcacggagct cttggggccg gcgcgcttcc tcgaagtagg
    11101 ggaagaagat gcgcgggtcg gcgccaggag acacgatatt gaacttcggg tcgaagcagt
    11161 cgattcccga aatgacgcga tagaggcggg gcagcgagaa cgagccgtag ctctcgtact
    11221 gaccgacgct ttcgtcagtt ccggcgatct cctggtaggt gctcgtgatg atgaagtctg
    11281 cggtattcat cgccatcagg tcggcggtgt actgcgcggc gaagtgatgg tccgcctcgt
    11341 ggttctgcca atggagatcc gagagcaagt acttcgtctt ctcgagggca tgcgcgatgt
    11401 tgcactgcgt gactccgaac ctgtgagcca gcacgctggc gaccaagttg ccgtctgagt
    11461 agttgccaat cacgatatcc ggccgtacgc ctcccagctc tgcgagcaac tcccgttcgg
    11521 cgtcgatcgc gtaccgctcg aggtacggcc agacttcgaa tctcgaaatc catttcgaga
    11581 tgatctcgcc accgtgatag gtgcgaaacg gaacgcgcag aatgcgagca ttctccgttc
    11641 caagaatcgg ttcgatgcgc tgatcgcagg tcgttccgtc ggattcggga atcaggcgcg
    11701 acaggacgac gatttgggga tcgatgtcga gcccctgttc gtagatcgag tcgcgcattt
    11761 ctttttcgag ggctcgaact tggtcgagga tgtagacgac ctgccctccg gtgtcgggct
    11821 tgccgagcac gttggactgt ccgaagtaac cgtgcggtga gatgatcgcg atcgagaaga
    11881 tcatcggaat gcgttctaga aagctctcta ggttttgcgg cgacggcgcc tcgagaatat
    11941 cgagaagaag ctccatggtt tcgcggatcc ggtcgggggc tcggccccag ccaggctcga
    12001 aaccgagcct gcggagctcg cgattgaagc cgttggtctc ggactcggac ccctcgagga
    12061 gcgcggtagc ctctgccagc gcttcgcgta gctcgtcgac gtctcgcagg gttccattga
    12121 gcatcagctg ctgcccgcgg acttgatgaa cctgtaggaa atcgaagagc agcttctggc
    12181 cgcttccacc gtcggtgaac aagcgcaccg ccagatgccg gttcaaaaac tcgacaccgc
    12241 ggccgatgct tcgcggatct ttgagcttgg ggaagcgccg ctcgaacgga ctgagatcga
    12301 tctcgaggac gtatcgttcg ccatgtggga tgcccgctgc gatgcgctct ttgatgtcga
    12361 gaaattcgtt caccgtgagc tcacgacagc gcatgtcctc cgtctcgacc tgcacgtagt
    12421 gccaacggcc cacacgcgcg cgcagggcca ggcagacgaa aggttcgtcg accaccgcct
    12481 cctgcgtgtg acgcatgatc cacgacatct tcgtctcgag aagcgaggcg cccacatccg
    12541 aggcggagaa gtcctcgaac tcgtg
//
LOCUS       DASRZF010000069         2461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3835534, whole genome shotgun sequence.
ACCESSION   DASRZF010000069 DASRZF010000000
VERSION     DASRZF010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3835534"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1657
                     /locus_tag="VFG22_03075"
     CDS             <1..1657
                     /locus_tag="VFG22_03075"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET6415254.1"
                     /translation="YTYNADSTAQTLLKDFDYQEGNQGVLWGTFGSGFLELNRDNFLN
                     ITSMYSRTSDDQTILQLGGEQNDNFIPLTKTSFNFIGRSIFFNQITGDHRNLKDSKVR
                     LQWNAVVSTGKRDEPDRRLIEQQVESQTVTYASRFFSDLEQLSIGGKTSARFPVYQAF
                     ESTAYATIGFNAGYTDRSFNARRFTQQGRGGSALIGDPGVLLGPDALGPVSTIKEVTQ
                     PEDSYVANNLLVGGYAQLETPVAPRMQFLGLLRFEAFRQEVTSNSPFSDDDNEPQGTN
                     RQDLNPMPSANLSIEINEKMFVKLGYGMTVIRPAIRELAPFAYLDFLRGWLVQGNPEL
                     KPTQVQNAEARYEYYFGDTDLIAATAFGKYFVDPIEFVILSQVNGNASYRNAESAWLV
                     GGEVELRLGFGRFHDKLERFYFLGNVAVMGSQTTLPANQQIAGRLQRRLFNQSPFVTN
                     LSLRFDDPGAGVMVGLIYNAFGPRIVEAGSPQGGDVIAPDVFQQTQHQLDLIATWNVT
                     EHVKLGFKWKNIALARVRYKQGDELTFLQNWGSTVSIGAEYIY"
     gene            1725..>2461
                     /locus_tag="VFG22_03080"
     CDS             1725..>2461
                     /locus_tag="VFG22_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415255.1"
                     /translation="MRKYFGIVLASAMAAASAGLVGCSGDGGNGGTGGTGGTAGSGNP
                     CQDNGGSGLVEISDEEITQDTVLEAACTYVLTTETYVVGAELSIEAGTEIQGDQGSAL
                     IVTNTARIDAQGTSEAPIVFTSSALPSQAASGDWGGIVLLGLARLSWGDTTCDGEAGE
                     CVGSVEGIAPGNPRGAFGGDDDTHDCGTMRYVRIEYAGFQFGGDNELNGLTVGGCGDQ
                     TVLSYIQVHRGLDDGVEFFGGTAPIDHL"
BASE COUNT          545 a          731 c          722 g          463 t
ORIGIN      
        1 ttacacgtac aacgcggaca gcaccgcgca gacgctgctc aaggacttcg actatcaaga
       61 gggcaatcag ggggttctct ggggaacttt cgggagcggc ttcctcgagc tcaatcgcga
      121 caacttcctg aacatcacga gcatgtacag ccgcacgagc gacgatcaaa ccatcctgca
      181 gcttgggggc gaacaaaacg acaacttcat cccgctgacc aagaccagct tcaatttcat
      241 tggacgatcg atcttcttca accagatcac tggggatcac cgcaatctaa aagacagcaa
      301 agttaggctt caatggaacg ccgtcgtcag cacgggcaag cgcgacgagc ccgatcgccg
      361 gctcatcgaa caacaggtcg aatctcaaac ggtcacgtac gcttcccggt tcttctccga
      421 cctcgagcag ctctcgattg gcggcaagac cagtgcgcgg tttcccgttt atcaggcgtt
      481 tgaatccaca gcatacgcaa caataggctt caacgcgggc tataccgatc gtagcttcaa
      541 tgcccggaga tttactcagc aaggccgagg cggttcggcc ctgatcggcg acccaggcgt
      601 gttgctcggt cctgacgcac tcggacccgt cagcacgatc aaagaagtca ctcaacctga
      661 ggatagctac gtcgccaaca accttttggt tggaggatat gcacagctcg aaacgcccgt
      721 ggcgcctcga atgcagtttc tcggcctact tcgattcgaa gcattcaggc aggaggtcac
      781 ttccaacagt cccttcagcg atgacgacaa cgagccccaa ggtaccaacc gacaggacct
      841 caatccgatg ccctccgcga acctctcgat cgaaatcaac gagaagatgt tcgtcaagct
      901 cggctacggc atgaccgtga ttcgtcccgc gatccgggag ctcgcgccat tcgcttacct
      961 cgatttctta cgcggatggc tggtccaagg caaccccgag ctgaaaccga cgcaagtcca
     1021 aaacgcggag gcacgatacg agtactattt cggcgacacg gatctgatcg ccgcaacggc
     1081 attcggcaag tacttcgtcg atccgatcga gttcgtgatc ttgagccaag tcaacggcaa
     1141 cgcgagctat cgaaacgccg agtcggcgtg gctcgtcggc ggcgaggtcg agctgcggct
     1201 cggctttgga cgatttcacg acaagctcga gcgtttctac ttcttaggca acgtcgcggt
     1261 gatgggctcg cagaccacgc ttcctgcaaa tcagcaaata gcgggccgct tgcagcggcg
     1321 actgttcaac cagtcgccct tcgtcacgaa cctgtcactt cgttttgacg atcctggtgc
     1381 gggcgtgatg gtcggtctga tctacaacgc atttgggcct cggatagtcg aggcgggaag
     1441 ccctcagggt ggcgacgtta tcgcgcctga tgttttccag cagacgcaac accagttgga
     1501 tctgatcgcg acatggaatg tgacggagca cgtgaagctt gggttcaagt ggaagaacat
     1561 cgcgctcgct cgcgtgcgtt acaagcaagg tgacgagctc accttcctcc aaaactgggg
     1621 gtcgacggta agcattggcg ctgagtacat ctactgagcg tgactgaagg aatgaaaaag
     1681 actcacgcgg cgcaaacgac gctgccaaat atgaggttag ggatatgaga aaatactttg
     1741 gaatcgtcct tgcgagcgcc atggcggcag cgagcgcagg cctagtcggc tgcagcggtg
     1801 atgggggcaa tggcggaact ggcggaaccg gtggaacggc gggtagtggc aacccctgcc
     1861 aggacaacgg cggaagcggc ctcgtcgaga tcagcgacga ggagatcacc caggacactg
     1921 tcctcgaagc cgcgtgcact tacgtcctga ccaccgagac ctacgtcgtc ggcgccgagc
     1981 tcagcatcga ggccggcacc gagatccagg gcgatcaagg ctccgctctg atcgtgacca
     2041 acaccgcccg gatcgacgca caaggcactt ccgaagcgcc gatcgtgttc acctcgtcgg
     2101 ctctcccgag ccaggctgcg tcgggtgact ggggcggtat cgttctcctc ggcttggctc
     2161 ggctcagctg gggtgacacg acctgtgatg gtgaggctgg cgaatgcgta ggtagcgtcg
     2221 agggcattgc tcccggcaat ccacgcggtg cctttggtgg cgacgacgac acccatgact
     2281 gcggcaccat gcggtacgtg cgaatcgagt acgcggggtt ccagttcggt ggtgacaacg
     2341 agctcaacgg cctcaccgtg ggcggctgcg gcgaccagac cgttctgagc tacatccagg
     2401 tacaccgcgg cctcgacgac ggggttgagt tcttcggcgg aaccgcgcca atcgaccacc
     2461 t
//
LOCUS       DASRZF010000070         5818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4868559, whole genome shotgun sequence.
ACCESSION   DASRZF010000070 DASRZF010000000
VERSION     DASRZF010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5818
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4868559"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="VFG22_03085"
     CDS             <1..776
                     /locus_tag="VFG22_03085"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6415256.1"
                     /translation="GGCALVTGAARGIGRAFAQLLAAEGFDLLLVDREEAETKALADE
                     LHGTYGIEATAQISDLAKPDLENEARSWAQRYEIGLLVNNAGLSLLDPFFEISLDDHL
                     STLDLNCRATLVLTHIIGQRMRDRGRGGIIVVSSASALSGSPYFCHYAATKAYGLNLA
                     AGLWSELRGSGVDALAVCPGMTDTKPVQDQKLDQNLPFYIPLSDPKSVALAALGALGR
                     QPALVPTWTDRVSTGLLSKLLPRRWTLSIIKRSIEKMRG"
     gene            complement(773..1699)
                     /gene="gloB"
                     /locus_tag="VFG22_03090"
     CDS             complement(773..1699)
                     /gene="gloB"
                     /locus_tag="VFG22_03090"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR03413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HET6415257.1"
                     /translation="MIVLSGAGTAFCSGYDLAYYAEASGSASGTQEIPWDPMKDYAFM
                     MRAGVWSAARFKPIVPAMQVIPVPCLSDNYAYLVIENGRAAVVDPSQADPVLRAIEEA
                     RVKLSEIWLTHHHWDHIGGIESLIEECPIKNVRGSRYDAEHRRIPKQTDALSDGDAFD
                     FAGATVQVLEIPGHTLGAIAFIADGNLFSGDTLFVAGCGRVFEGTMEMMSRSLGKLRL
                     LPPLTKIWCGHEYTVNNLRFAQTVEPENREIDRALEDALAARSAKRFTVPGRIDRELA
                     TNPFFRFDDPKVAAGKDPVASFTAIRKAKDDF"
     gene            1700..2233
                     /locus_tag="VFG22_03095"
     CDS             1700..2233
                     /locus_tag="VFG22_03095"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiD C-terminal domain-containing protein"
                     /protein_id="HET6415258.1"
                     /translation="MNVLIRIRACAAPFLDSVVGKPVWCLYTSDMDYKWAIEKGIAFA
                     GRTEVQVLDLKPGYVKMLMPLKPNLNHVGTMYAGALFTLAELPGGAIFLSTFDSSRFF
                     PLIKGMDIEFRKPAATDITVEVRLDPEEAASIQAKADELGKADYGWECDLKDANDEIV
                     AVSSNRYQLRSMKPPGA"
     gene            2226..2846
                     /locus_tag="VFG22_03100"
     CDS             2226..2846
                     /locus_tag="VFG22_03100"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HET6415259.1"
                     /translation="MLEADDNLRARIRANLVAHRRSVEPDSSLRPAAVAMTVVANDRG
                     EASFIITRRVSSLRNHSGQWALPGGSIDEGENAVGAALRELDEEVGLKCGPDRVLGLL
                     DDYPTRSGFRITPVVIWAGGDAALKPDPNEVAAAYRVPISGLDAPGIPKLFDLEESDR
                     PVLSVHILGQDIFAPTAAVIFQFREVAIHGRDTRVAHYDQPRFAWR"
     gene            2894..3373
                     /locus_tag="VFG22_03105"
                     /pseudo
     CDS             2894..3373
                     /locus_tag="VFG22_03105"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
     gene            4930..5448
                     /locus_tag="VFG22_03110"
     CDS             4930..5448
                     /locus_tag="VFG22_03110"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET6415260.1"
                     /translation="MRARSAWVIVIVLGFTMTFALVMRHPGTWKALISARFPGIEWVD
                     SETLSQWMDSASNEELVLLDVRSLEEQEVSQLRGAQRLDARQPDLDSLAIPEGAKVVV
                     YCSIGYRSAALVEDLKRAGVAHVYNLDGGLFDWANQGRPIYRGEERVEEVHPLNRIWG
                     LLLSPDLRAEPN"
BASE COUNT         1146 a         1751 c         1815 g         1106 t
ORIGIN      
        1 acggaggttg tgcgctcgtc actggcgcgg cgcggggaat aggacgcgcg ttcgcccagc
       61 ttctcgcggc tgaaggcttc gatctcttgc tcgtggaccg ggaagaggcg gaaacgaagg
      121 cgctcgcaga cgaacttcac gggacctacg gaatcgaagc cacagcccag atctcagatc
      181 tggcgaaacc ggacctggag aacgaggccc gaagctgggc acaacggtac gagattggac
      241 ttctcgtgaa caacgcgggc ctttcattgc tcgatccgtt cttcgagatt tcgctcgatg
      301 accacctcag caccttggat ttgaactgtc gggcaacgct cgtactcaca cacatcatcg
      361 gccagaggat gcgcgaccgc ggccgcgggg ggatcatcgt cgtctcgtcg gcatcggcgc
      421 tttcgggctc tccttacttc tgccactacg ctgccaccaa agcgtacggg ttgaacttgg
      481 cggcgggctt gtggtcggag cttcgcggaa gcggggtcga cgcactggcg gtctgtcccg
      541 ggatgaccga tacgaagccg gtgcaagacc agaagctcga ccagaacttg ccgttctaca
      601 tcccgctgag cgatccgaag tcggtcgcgc tcgccgctct gggtgcgctc ggccgacagc
      661 ccgcgttggt tccaacctgg accgatcgcg tgtccacagg attgctttcc aagcttctgc
      721 cgcgacgctg gacgctctcg atcatcaagc gctcgatcga aaagatgcgt ggctagaaat
      781 cgtctttcgc cttgcgaatc gctgtgaatg aagcgaccgg atccttgcct gccgcaactt
      841 tcggatcgtc gaaacggaaa aacgggttgg tcgccagctc tctgtcgatc cgacctggta
      901 cggtgaaccg cttcgcgctg cgagccgcaa gtgcatcttc gagagcgcgg tcgatctcgc
      961 ggttctctgg ctcgacggtc tgggcgaatc gcaggttgtt gaccgtgtac tcatgaccac
     1021 accagatctt cgtcagcggc ggaagcaagc ggagcttgcc gagcgagcgc gacatcatct
     1081 ccatcgtgcc ctcgaagaca cgcccgcaac cggcgacgaa aagggtgtcc ccactaaaca
     1141 gatttccgtc ggcgatgaat gcgatcgccc cgagcgtgtg tcccggaatc tcgaggactt
     1201 ggacggtcgc acccgcgaag tcgaaggcgt cgccgtcgga gagcgcatcg gtttgtttgg
     1261 gtatccttcg atgctcggcg tcgtaccgcg agccacggac attcttgatc ggacactctt
     1321 cgatcagaga ttcgatccct ccgatgtgat cccagtgatg gtgtgtgagc cagatttcgc
     1381 tgagctttac cctagcttct tcgatggcgc gaagaacagg atccgcttgc gaggggtcga
     1441 cgacggcagc ccggccgttt tcgatcacca agtaggcgta gttgtcgctc aggcagggca
     1501 ctggaatcac ttgcatggca ggaacgatag gcttgaagcg cgcagctgac cagaccccag
     1561 ctctcatcat gaacgcgtag tctttcatgg gatcccaagg tatctcttgc gtaccggatg
     1621 cagagcccga tgcctcggca taataggcga gatcgtaccc tgaacagaac gccgtacccg
     1681 caccggaaag aacgatcacg tgaacgttct catccgcatt cgcgcatgcg ctgctccgtt
     1741 tctcgactca gtcgttggaa aaccggtctg gtgcctttat accagcgaca tggactacaa
     1801 gtgggccatc gagaaaggga ttgcgttcgc tgggcgcacc gaggtgcagg tcttggatct
     1861 aaaacctggg tacgtgaaga tgctgatgcc gctcaagccc aatctaaatc acgtcgggac
     1921 catgtacgcg ggcgcattgt tcacgctggc ggagcttcct ggcggcgcaa ttttcctgtc
     1981 gaccttcgac tcgagtcggt tcttccccct gatcaagggg atggacatcg agttccgcaa
     2041 gccggccgca acggacatca cggtcgaggt gcggctcgat ccggaggaag ccgccagcat
     2101 tcaagcaaag gccgatgagc tcggcaaagc cgactacggc tgggaatgcg atctcaagga
     2161 tgcgaacgac gagatcgtcg cggtctcctc gaaccgatat caacttcgaa gtatgaaacc
     2221 gcctggtgct tgaggccgat gacaatctgc gtgcgcggat ccgcgcaaac ctggttgcgc
     2281 accggcgctc ggtcgaaccc gactcgtccc tgcgacccgc cgccgtcgca atgacagtcg
     2341 tcgcaaacga ccgaggagaa gcctcgttca tcatcacgcg ccgcgtatcg agcctccgta
     2401 accacagcgg acaatgggcg cttcccggag gaagcatcga cgaaggtgag aatgccgtag
     2461 gcgcggcgct ccgagagctc gacgaagaag tcggcctcaa gtgcggaccc gaccgggtgc
     2521 tcgggcttct agatgactac ccgactcgct cgggctttcg gatcacgccg gtcgtcatat
     2581 gggccggagg cgacgcggcg ctgaagccgg acccaaacga agtggccgcg gcgtaccgag
     2641 ttccaatctc aggcctcgat gctccgggca tccccaagct cttcgatctc gaagagagcg
     2701 atcgacccgt tctgtcagtt cacatcctcg gccaagacat cttcgccccg acggcagccg
     2761 tgatcttcca gtttcgagag gtcgccatcc acggccgcga tacccgagtt gcccactacg
     2821 accagcctcg attcgcctgg cgctgatccc ggttgacctg agcttgccaa gcgccaaatc
     2881 atgggagatc cgcatggcga atcatcttca gaacgaaacg agcccctatc tactgcagca
     2941 cgtcgagaac ccggtggact ggtatccctg gggcgaggag gcgatgcggc tcgccgctga
     3001 acacgacaaa ccgatcttgc tgagcatcgg ctatgcggcc tgtcactggt gccacgtgat
     3061 ggcgcacgag tccttcgagg acgaggccac ggcggcgcag atgaacgccg agttcatcaa
     3121 tgtgaaggtc gaccgcgagg agcggccgga catcgacagc atctacatgc aagccgtcaa
     3181 tgcgatggtc ggccgtggcg gctggccgat gaccgtgttt ctgacgcccg atggcaagcc
     3241 tttttacgct ggcacttact ttccaaacga acctcgacac gggatgccga gcttcaggca
     3301 gatcctaacc ggtgtgaccc aggcctggaa cggcgacaga gagagagtgc tcggcagtgc
     3361 cagtgaggta gcagagcagc tccaggcgct ctccgacgtc ggcttcgacg aagggacgct
     3421 cgatacggac gtctttcgaa gcgctatgcg cgggctgagt cggcgcttcg acgcaacctg
     3481 gggcggcttc ggcgatgcgc ccaagttccc acaacccatg accctcgagt ttcttcttcg
     3541 cgaatcgatt cggtcgggcg acgcgagtgc gctcgagatg gccgaggtga ccctccgaaa
     3601 gatggccgag ggtgggatgt acgatcatct agggggcggg ttcgctcgct actccgtgga
     3661 tcaccgttgg ctggtgccac acttcgagaa gatgctctac gacaattcgc agctcgccag
     3721 agtctatctc cacgcctggc agctcaccgg tgaggagctc taccgacggg tcaccgaaga
     3781 gacactcgac tacgtgctcc gagagatgcg tcacgcagac ggaggcttct acagcagtca
     3841 agacgcagac agcgagggca tcgaaggaaa gttctacgtc tggagcgccg acgagatccg
     3901 cgaggcgctg ggcgacgacg ctgagacatt tatgaagagg tatggcgtat cggacggtgg
     3961 caactgggag ggctccaaca tcttgaacct gcgcgtcgac ctcgcgagcc tcgccgagga
     4021 gatggaggtc ccggtcgacg ccctcgacgc acggatgagc gccgctctcg caaagctcta
     4081 cgaaattcga tcggcgcgca tctggccagg tctggacgac aaggtactca cgtcgtggaa
     4141 cgggctgatg ctcggtgcgt ttgccgaagc gggactggca ctagatcgcc cggactacgt
     4201 ggaaacagct aggtccaacg cagactttct ctaccgcacg atgcgctgcg agtcgggccg
     4261 tcttcttcga acctggagag ctcggtccga agccaagtac aatgggtacc tcgaggacta
     4321 tgcttatctc gccgacggtc ttttggccct gtacgaggcc accttcgatg aacgttgggt
     4381 gggctgggca cgagagctcg cagagatgat gctcgtccac tttcgcgacg tggtgaacgg
     4441 tggtttcttc gacacctcgg atgatcacga gcagctgatc cacaggccaa aggaccttca
     4501 agacaatgca gtgccgagcg gaaacgcgat ggcggcgagc gtgctcctca aactcagcct
     4561 gctcacgggc aacggcgact actggcagat cgccgaaaag agcgtggcta cgatggcgaa
     4621 attcatgagc gagtacccat cgggtttcgg acattggctc gcgacggcaa cgttcgttct
     4681 gagcgagccg caggagctcg cgctgatcgg cactgacgaa gacatcgcgg cgctgctcgc
     4741 ggtggcgcgt gacgcgtacc gcccctttca agtgattgcg gccaccgaag aagacgcgca
     4801 atcctccctg ccactcctgg ctgaccgtcc acgcatcgaa gggaaaggaa ccgcctacct
     4861 ctgtcggcgg ttcgtgtgtc aggcaccgac gacggacgct gagcagttga gagtgcaact
     4921 ggagtcggct tgagagcccg atccgcgtgg gtcatcgtga tcgtgctggg gttcacgatg
     4981 actttcgcgc tcgtcatgag gcatcctgga acttggaagg cgctgatctc cgcgcgcttc
     5041 cccgggatcg aatgggtcga cagcgagacc ctctcgcagt ggatggacag cgcgtccaat
     5101 gaggagctcg tgctgctcga tgtgcgaagc ctggaggagc aggaagtgag ccagttgcgc
     5161 ggagcgcagc gcctcgacgc gagacagccc gatctggatt cgcttgcaat acccgaaggc
     5221 gccaaggtcg tcgtctactg ctcgatcggc taccggagcg ccgcgctcgt cgaagatctg
     5281 aaacgggcgg gtgttgcgca cgtttacaac ctcgacgggg ggctcttcga ctgggcgaat
     5341 cagggtcgtc cgatctatcg tggagaagaa cgcgtcgagg aagttcatcc tctcaatcgc
     5401 atctggggct tgctgctgag cccggatctg agggcggaac ccaattgagc cggacgaacc
     5461 ggcggcggcc gggcgtgctc ggctagcggg cgggcggttc gtcgatgacg acaggattct
     5521 tcgcaggcac attcggcgta ccggtcggcg gtcgaggttt cgaaggaggc ggcgaagcgg
     5581 caggttcgct cggcgccgcg ttcgcagctg ctggcggctc ctgaggaaaa tcaggaacgc
     5641 tccaatcgat cccatcatcg ggcgcggcgc cctccccagt ctctgtcgct gccgctgtcc
     5701 ctgccgctgt ccctgtttcc actgccgttc gcttcgtcgt cgttctcttc ttcttcttcg
     5761 gattcggctc cagctgcgcc tccaaaacgg gttcgtctgg aacggcttcc tcgacagg
//
LOCUS       DASRZF010000071        14717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4868714, whole genome shotgun sequence.
ACCESSION   DASRZF010000071 DASRZF010000000
VERSION     DASRZF010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 14717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14717
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4868714"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="VFG22_03115"
     CDS             <1..676
                     /locus_tag="VFG22_03115"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET6415261.1"
                     /translation="RIDGYNLTVPHKEAILSLLDEVDTTARAIGAVNTVVREGERYIG
                     HNTDAPGFVRSLEEAGLSPLDAQVCILGAGGAARAAVLGLATAGASEISVLSRRPEQS
                     QALCDSLAPHVSCKLVPRPLGEARDAFEGATLLVQASSATLESNPGAQAFAASLPMEA
                     LPQTAAVVDMVYKPLETAVLERARSLGLSVVDGLGMLVHQGAIAFEMWTGFEPPLEVM
                     RAALKD"
     gene            complement(683..1630)
                     /locus_tag="VFG22_03120"
     CDS             complement(683..1630)
                     /locus_tag="VFG22_03120"
                     /inference="COORDINATES: protein motif:HMM:NF013246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005254 - chloride channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HET6415262.1"
                     /translation="MIVRGGTGYKRAFKHYILGNWPLILTVLVVSRAIPWVAEYTDVL
                     QTDVFSATAIGLLATVVGIFIVFRFNEAYQRWWEARILWGALVNESRSFCREVITLLS
                     AERTPEIGSAEAIEALKRELVHRHLAYCNALRLSLRGQDTWDELAPFLSAEELNELKS
                     YANKPTQLNRTQGERLARSLGLDTPQQLVLMRFDARLSALYDIQGGCERIKNTAFPDQ
                     VRFISQSLVWLGAIAVPIAFLGTDQDIRVMEVLAVIVISLSFMVVEQLGAALKNPFES
                     EPNDTPMTALCRTIEIDLRQQLGESEIPPPIEPIAGVLM"
     gene            complement(1627..2085)
                     /locus_tag="VFG22_03125"
     CDS             complement(1627..2085)
                     /locus_tag="VFG22_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415263.1"
                     /translation="MRLFTPTGWVIGTLHVPDGLGLLPFLNGEEAFFRMTNVSLPEQP
                     RTIPFLALQRKAVLIVVPGEDALLGLHEDDGRDRHEVACLFDGGMVMGTLPLPRGVRV
                     SDELMQSQEFFAIEDCTLAIDASPEPLMEAEELVLVHGAEMFGLAELEDE"
     gene            complement(2136..3629)
                     /locus_tag="VFG22_03130"
     CDS             complement(2136..3629)
                     /locus_tag="VFG22_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 factor interaction domain-containing
                     protein"
                     /protein_id="HET6415264.1"
                     /translation="MLDADMRARLEAIGKLGFTNPFGPDRPKLEAIIVGEQEARRSLW
                     NRSLDWPREDPVLQAIQSEAERLISAARAAIARGVAYGEQDQELIRGATLYALYYRYD
                     APLYAWINSEAPGDPVPFYRDFEKDFHALLRHVGPPSWAATPAELIAIFFQTRRAFHF
                     VFHQILGTSLAAAALRASVWESLFTYDPWRYLRQLRGRMHESSTLILGPSGTGKELVA
                     SAIGLSSFIPFDEQRGRFARNFRSAFHPIHLAAMSKTLIESELFGHKKGAFTGATGDR
                     MGHLEGRSASDAIFLDEIGELDPEVQVKLLRVLQSRTFHRLGDSEPRTFQGKVIAATN
                     RSLSDEMSAGRFREDLYYRLCSDVICTPSLEDQVGGNADELGHLVRVLVQRTLGDSDE
                     ATFDEVMRGIDQSVGRDYAWPGNMRELEQCVRNLLIHGQYLPPRAPQKLDALDSLLQS
                     MRSAEATLDEVVSKYSNWVYTRERSYARAGEILGVDRRTVAKHVSDS"
     gene            3757..4392
                     /locus_tag="VFG22_03135"
     CDS             3757..4392
                     /locus_tag="VFG22_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415265.1"
                     /translation="MNRMKRWTMSVTSWVDGVLAQVENHEAAVGAAIGRVRQSTARAR
                     VQLKRVERDQLTLRDSLLKEEEALDAWRRRAKVLEDEAAAIECMRRCKASERRVLLLR
                     QRLAEHERSHKELSEGIRVLNARLSELNERKNLMRTRQSRAEAAHGMAGATGPIGDLE
                     DVFDRWETRVGEIEIAAECAEPVDAFEAELEASDEAAALRLELAALRAEEL"
     gene            4401..5633
                     /locus_tag="VFG22_03140"
     CDS             4401..5633
                     /locus_tag="VFG22_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415266.1"
                     /translation="MDTRLQQLFQVLRWCGAAMIVAAAGTFLIQSWEQVGDVTRYLTL
                     LGMTALLPALAYVCGIRLQEGRSARVLMLTLLALIPIHAGVLGGFVLSQFGTTTENLA
                     PIAHWVAPSRSAAILLVAGAALALIPLIWASFRVLARPHARLLTGLSAAGHALLLVPN
                     RSALGAMLTVVPILGAAAWSANRVKPATLESRLAVSSLVTPAVVIAARQFLFYEVTSA
                     FWGTILGISALALFMLGEKISDTSLERVAFAPALMAIGAFIDAAQVVNRWSFSTTLLS
                     YGIFATIPLLAFAWRSARSRAFFVRCAMILNAATATTTLLVAPRPWAALQCLALGLAL
                     LSYGFIRDRRAALYSGISLAAFGFLVEVAHAVEVFQPSGWLALAGFGFVLVGLTAWLE
                     RRSSAAKLSQELAPGLPQ"
     gene            5731..6171
                     /locus_tag="VFG22_03145"
     CDS             5731..6171
                     /locus_tag="VFG22_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415267.1"
                     /translation="MRRTGIYLLVVLSLVVGAAVVTDAIVVTDRERMDQFIESVTGQV
                     SGSRIDNALSYADPSKVTMELVHDGHRARYSNRNAAKLKSDARKALASLEGSRLHLIQ
                     ESVSLDGERARIALRLRTDAGLANTVFDLRRENDVWFLRRVIVN"
     gene            complement(6168..6458)
                     /locus_tag="VFG22_03150"
     CDS             complement(6168..6458)
                     /locus_tag="VFG22_03150"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HET6415268.1"
                     /translation="MTELEIREQEGAITFEVRVAPRASRNRVLGVQEGALKVALTAAP
                     VDGAANEALRKLLAKALGVSKSDVEIVRGARARNKVLRVHGVSARDVRFGEA"
     gene            6513..7526
                     /locus_tag="VFG22_03155"
     CDS             6513..7526
                     /locus_tag="VFG22_03155"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HET6415269.1"
                     /translation="MHPVLFSIPTPWGAIPIYSYGVMLGSSLLFAWYFIMYMGKKIEG
                     YNRELLASCFTWTAVGAIIGARFLYILTNLDSYESLGSWFDLRSGGLVAYGGFLGGFI
                     TAWSFWRIKKIPLLPFADLAAPTLASGLMLTRVGCYLYGCDYGRPLLESAPAWLQKAG
                     TFPKWDTEAYPAFACDQVISGSPAYQHHLQEYPEAMIDRLSSLAVHPTQIYESLFGLL
                     LFVFSVWLLTHRKFRGQVLVTVAGIYGLWRFLIEYLRDDPERGFAFGFSTSQLISLAI
                     IPVCIIAYIHLKKNAAENGDIELPAWALETARGPAVSPSAATSADGRPSKYKPKKIKK
                     KKN"
     gene            complement(7533..8462)
                     /locus_tag="VFG22_03160"
     CDS             complement(7533..8462)
                     /locus_tag="VFG22_03160"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HET6415270.1"
                     /translation="MKPVGLRFLTLAAVIGFAAAASIADAQAVYTHAVQSGDTLASIA
                     QRYYGDPTREAVLREANRMKGPGSNAMVQGSWLFIPMVTFYQVGKDETWKSIAAKLYG
                     DEGRATILIEANSGNRRVEPDEGAELLVPYPLRHVVGPGETLVKIAGMYMPNSPLSLK
                     RLRRFNSGAEIERGQIVLVPLFDLRLAGEGRAEASAAFMRAAGGGATKAAQDEVESAL
                     PGLIEQGQKAAFAEAIALGNRLLGKNNLTSSQMVTIQKELAVAYVAVERTDLAEASFR
                     AALALQPNLELDTVRTSPRVLEAFNRAKQPTDE"
     gene            complement(8459..9787)
                     /locus_tag="VFG22_03165"
     CDS             complement(8459..9787)
                     /locus_tag="VFG22_03165"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6415271.1"
                     /translation="MVERIGTCRVLGEIGSGGMAVVYEAIQEPLNRRVAIKALKPSIA
                     IDSQFAERFEREAHFMASLQHENILHVIDFIKDGRSMFIIMEYVDGIDLYDLLERSPR
                     LPADVAAIIMLQLVRGLDYAHFRGIIHRDIKPANIMVSVQGDVKLMDFGIARHERFGD
                     LTETGTGLGTPSYMSPEQILGDKLDFRSDIFSVGIVLYQMLTGRKPFVEDDSRTVMQK
                     IRLDRFESPKKLGVDVPRKLERIMARCLQKMPANRYPTTQALIDDLTEFLAPRVPMSH
                     NARLVMYLSEIGVMTGDQAEEVLSSVAPRVLRSGRRDVGFLKNVAKWQAAAAVLILVS
                     GLTVQAASGRWSEPAVGDAPVTLNPADAGYLTVSADPWAHVQVDGQHVLTTPSARSIP
                     LTPGRHYVKYSNPYFAPVEHEVVVEAGTTVPDRATLSEAINVVADPEATP"
     gene            complement(9859..10989)
                     /locus_tag="VFG22_03170"
     CDS             complement(9859..10989)
                     /locus_tag="VFG22_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415272.1"
                     /translation="MRDERGMKAGNERLRRWAITGLFALGLIVACSDSFLGPWAVTDL
                     RVVAAVVDVDGEPGRANPEPGDGVEVSLRVIDKGSPPSDIEGVPALTPEPLEWLFAAC
                     LPLPTLIGAPICADPRAIIQPCEGCVGTPPADPFAFPVMRFQVPSESEVEAAQATSVL
                     LQGIVCVNGVPSTDAIQRFLAGESDDLEPCVPRAEPGREDEPPDGRFVVVQIPIETDA
                     SDPNLNPEIDQVRYEGTAWPPPYDQGVPRTAPRTGCRADLDSLTPEERAMHPVAGGPA
                     ADIRLQVTRESLQTYTVDDKTLTEEIQITWLTDGGGLVQTFSFITTPAIDSLTQWTPP
                     TGVEEDGQLVRFNFVIRDGRGGTDWVERGLCVLPAPAAESPP"
     gene            complement(10989..13574)
                     /locus_tag="VFG22_03175"
     CDS             complement(10989..13574)
                     /locus_tag="VFG22_03175"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET6415273.1"
                     /translation="MLRFLAAFLLVSIVSSATYAQTSFQLAPNEEKKPLVTPPKLVRF
                     VEAAYPKTEGEEPVEAAVELDIVVGKDGLVTEVSVARSAGEAFDEAALAAARQFVFEP
                     ARKDWEPIAARIRYRYIFELKEPPEEITTAWLSGTVLLAENESAAGSVSIEILNERNE
                     LVRELVAGPDGTFVVTDLEPGTYEVTILGGEFGDLKAEEELAAGQVTQVIYRLGAQKK
                     ASYRGFGETAVVEAPPREVVKRTIDREELTRIPGSRGDALRAVELLPGVARPPFGLGL
                     LIVRGSAPQDSESFIDGVPVPLIYHFGGLTSVYNSYALEKIDFYPGNFSVRYGRRTGG
                     ILEVTTKDPARDRVHGVAEISVIDTFFTVEAPITDKIQIAAGLRRSFIDLILPAVLPD
                     DIGVRQAPVYIDYQFKLNFLPSKRDRVRISAYGSNDRLELFLEEPSSDDPAIRGNTAL
                     GSSFYNNQIDWARRVGDKVEQDLAFNVGPTKINLSLGDAFQLRGSFIQTYGRAEWRYQ
                     ITDKIRLISGLDVFVAPVNLTYIGPQPGSQEGAGSAQGSLAGQPQVFADVSATVYRPG
                     VYLESDMELGKLTRLILGLRLDYFGDISAFTVDPRLTTIFTVAKDMRVKLGVGLFSQP
                     PEFQESDVTIGNPNLDPIQAVHVGAGWEYDAFPGVRFGVEGFYKYLWDRTIATPNGEA
                     PFYVTGGIGRIYGGEFSINLRPATGRQYFGYLSYTLSRSERKDGELEPWRLFDFDQTH
                     ILTLAFVYNFRRNWEIGGTFRLVSGNPNTPVIGSVYDALSDVYIPIDGRVNSIRNPLF
                     NELNLRVQKKWILDGWRLAVFLDIRNAYNAKNQEGIIYNYDFSQQTPLYGLPIIPALG
                     VRGEF"
     gene            complement(13920..>14717)
                     /locus_tag="VFG22_03180"
     CDS             complement(13920..>14717)
                     /locus_tag="VFG22_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415274.1"
                     /translation="CGDQTVLSYIQVHRGLDDGVEFFGGTAPIDHLIVTGPGDDGLDW
                     DQGWRGTADNVIVHHFSSTSSSPNGIEADNYGSGNNNVQPRSAPQVRNATVIGDGRPE
                     GSGVVTRVGTWGILDGLVVVDFGGAGYDMRNGAWSVAGGWPDGIVVTNSCFNDNTPNF
                     PADVDCIEDPENPGNAIGAEDCNDYSGSGDFTDEANYFPENTNMPTQGNLEEDPELGD
                     FSTAATGGNPAPDYSLANANCLGAFGGPSGTDWTSDWTAYPEGAAGL"
BASE COUNT         2686 a         4533 c         4651 g         2847 t
ORIGIN      
        1 gcggatcgac ggctacaacc tcacggtgcc tcacaaagag gcgatccttt cacttctcga
       61 cgaagtcgac accactgcgc gcgccatcgg cgcggtgaac acggtcgtgc gggagggcga
      121 gcgctacatc gggcacaaca ccgacgcacc cggattcgtt cgctcactcg aggaggccgg
      181 actgagcccg ctcgatgcgc aggtctgcat cctcggagct ggcggggcgg cacgcgccgc
      241 cgtgcttggg ctcgcgaccg ctggcgcctc ggagatctcg gtgctctcgc gccgacccga
      301 gcagagccag gcactctgcg actcgttggc tccgcacgtc tcgtgcaagc tcgtcccgag
      361 gccgctcggt gaagctcgcg acgcattcga aggggcgacc ttgctcgtcc aggcgagcag
      421 cgccacgctc gagtccaacc ctggggcgca ggcctttgcg gcctcgctgc cgatggaagc
      481 gctcccgcag acggcggcgg tggtcgacat ggtctacaaa ccacttgaaa cggcggtcct
      541 cgaaagagcg cgatcgcttg gcctcagcgt ggtcgacggt cttggcatgc tcgtacacca
      601 aggcgcgatc gctttcgaaa tgtggacagg attcgagccc ccgctcgaag tcatgcgcgc
      661 ggcgctgaaa gactgaagcc gctcacatca ggacgcccgc gatcggctcg atcgggggcg
      721 gaatttcgct ctcgccgagc tgctgtcgaa ggtcgatctc gatggtgcgg cagagcgcgg
      781 tcatgggggt gtcgttgggc tcgctctcga acgggttctt gagggcggcg ccgagctgct
      841 cgacgaccat gaagctgagg ctgatgacga tgaccgcgag tacctccatc acccggatgt
      901 cctggtcggt gccgaggaaa gcgatcggca ccgcgattgc gcccagccat accagcgact
      961 gcgagatgaa ccggacttgg tctggaaacg cggtgttctt gatccgttcg cagccgccct
     1021 ggatgtcgta tagggcgctg agccgcgcgt cgaatctcat caagacgagt tgttgcgggg
     1081 tgtcgagacc gagagaccga gccaagcgtt caccttgggt ccgattgagt tgagtcggct
     1141 tgttcgcata gctcttcagc tcgttcagct cttcggccga gagaaacggc gcgagctcat
     1201 cccaggtgtc ttgaccgcgc aggctcaggc gaagggcatt gcagtaagcg aggtgtcgat
     1261 ggacgagctc ccgtttgagt gcctcgatgg cttcggcgga accgatttca ggtgtgcgct
     1321 cggcagaaag gagcgtgatg acctcacggc aaaagctccg gctctcgttc acgagcgcac
     1381 cccagaggat ccgggcctcc caccagcgct gataggcctc gttgaacctg aacacgatga
     1441 agatgccaac caccgtggcc agcaggccga tggccgtcgc agagaacacg tcggtttgga
     1501 gcacgtccgt gtactccgcg acccacggaa tggcacggga aacgacgaga accgtcagta
     1561 tgagtggcca gttgccgaga atgtagtgct tgaacgcacg cttgtaaccc gtgccgccgc
     1621 ggacgatcat tcgtcttcga gctccgcaag gccgaacatt tcggctccat gcaccagcac
     1681 cagctcttcg gcttccatca ggggctccgg agatgcgtcg atcgcgagcg tgcagtcctc
     1741 gatcgcaaag aactcctggg actgcatcag ctcatccgat acacgcacac ccctcggtaa
     1801 gggcagggtt cccatcacca tccctccgtc gaacaggcac gccacctcgt gacggtcgcg
     1861 tccatcgtct tcgtggagac cgagaagcgc atcttctccc ggcacgacga tcagcaccgc
     1921 ctttcgctgc agggctagga acgggatggt ccggggctgc tccggcaagc tgacgttggt
     1981 catcctgaag aacgcctctt ccccattgag gaatggcagc agaccgagcc cgtccggaac
     2041 gtgcagggtt ccgatgaccc agcccgtggg agtaaacaaa cgcacggttg tcggagaggc
     2101 agacacgttc cgaggtctaa atacctgatc tcggcctagc tgtcgctaac gtgtttggcc
     2161 acggtccgac ggtctacacc gaggatttca ccggctcgcg catagctccg ttcccgcgtg
     2221 tagacccagt tgctgtactt gctgacgacc tcgtcgagcg tcgcttcggc gctccgcatg
     2281 ctctgcaaga gcgaatccag ggcatcgagt ttctggggcg cgcgcggggg aaggtactgt
     2341 ccgtgtatca agagattgcg aacgcattgc tcgagctctc gcatgttgcc tggccaggcg
     2401 tagtcgcggc cgacgctttg atcgatgccg cgcatgacct cgtcgaacgt tgcctcatcg
     2461 gagtcaccca gggtgcgttg cacgagaacg cggaccaagt gcccgagctc atccgcgttg
     2521 ccgccgacct ggtcctcgag cgaaggcgtg cagatcacgt cggagcagag gcgatagtag
     2581 aggtcttcgc ggaagcggcc cgcgctcatc tcgtcggaga ggcttcggtt ggtcgccgcg
     2641 atgaccttcc cctggaaggt ccttggttcc gagtcgccca ggcggtggaa cgtgcgcgat
     2701 tgaagcaccc gtagaagctt cacctgcacc tcgggatcga gctcaccgat ctcatcgaga
     2761 aagatcgcgt cacttgcaga ccttccctcg agatgaccca tgcgatcgcc ggtcgcccca
     2821 gtaaatgcgc cctttttgtg cccaaacaac tcggattcga tgagcgtttt ggacatcgcc
     2881 gccagatgga ttggatgaaa ggcgcttcgg aaattccgcg cgaagcgacc tcgctgttcg
     2941 tcgaagggaa tgaaggacga gagaccaatc gcgctcgcga ctagctcctt gccggtgccc
     3001 gagggtccga ggatcaaggt gctcgattca tgcatgcggc cgcggagttg tcgcagatag
     3061 cgccaagggt cgtaggtgaa caaggactcc cagacgctag cccgcagcgc ggcggcggcc
     3121 agagacgttc ccagaatttg atgaaacacg aaatgaaacg cgcgccgcgt ctgaaagaag
     3181 atcgcgatca gctctgccgg tgttgccgcc cagcttggcg gcccgacgtg acgcagaagc
     3241 gcgtgaaagt ccttttcgaa gtcacggtaa aatgggaccg gatcgcccgg cgcttccgaa
     3301 ttgatccacg cgtagagcgg ggcgtcgtag cggtagtaga gcgcatagag agttgccccc
     3361 cggatcaact cctggtcttg ctcgccgtac gcgacgccgc gcgcgatcgc ggctcgagcg
     3421 gcgctgatca atcgttcggc ttcgctctgg atggcctgga gcaccggatc ttctcgcggc
     3481 cagtcgagtg aacgattcca gagcgacctg cgagcctctt gctcgcccac gatgatcgcc
     3541 tcgagcttcg ggcggtccgg accaaaggga ttggtgaaac caagcttccc aatggcctcg
     3601 aggcgcgctc gcatgtcggc atccagcatg tacaagaagt gtactcacta tgtacgtatt
     3661 ctgtactctg aatttctctg ttctgtgatt aactgaataa tttcgggttg atcgatgctg
     3721 gcacgctccc tgctctggct gagcgaggag gctgcaatga accgaatgaa gcgttggacg
     3781 atgagtgtaa ccagctgggt cgacggagtt ctggcccagg tcgagaacca tgaagctgcg
     3841 gtgggcgctg cgatcggcag ggttcggcaa tccacggcga gagcgcgggt gcaactcaag
     3901 cgagtggagc gagaccagct gaccttgaga gacagcctgc tcaaagagga ggaggctctc
     3961 gacgcttggc ggcggcgtgc caaggtgctc gaggatgaag cggccgcgat cgaatgcatg
     4021 cgccgctgca aggcaagcga gcgcagggtg cttctcttgc gacaacgact ggcagagcac
     4081 gaacgctcgc acaaggaatt gagcgaaggc atccgtgtct tgaacgcgcg tctgagcgag
     4141 ctcaacgaac gcaagaacct gatgcggacg cggcagtcac gcgcggaagc agcccacggg
     4201 atggccggcg caacgggacc catcggcgat ctcgaagacg tgttcgaccg ctgggagaca
     4261 cgcgtcgggg agatcgagat cgcagcagaa tgcgcggagc cggtggacgc gttcgaagca
     4321 gagcttgagg catccgacga ggccgcagca cttcgattgg agctcgctgc cttgcgagct
     4381 gaggagctct aggaggtacg atggacacgc gacttcaaca actctttcaa gtcttgcgct
     4441 ggtgtggcgc agcgatgatc gtcgccgccg ccggtacgtt tctcatccag agctgggagc
     4501 aagtgggcga tgtcactcgg tacctcacgc tgctcggtat gacggcgctc cttcccgcac
     4561 tggcgtacgt ctgcggcatt cggctgcagg aaggacgcag cgcccgcgtt ttgatgctga
     4621 cgctcctggc gttgatcccg atccatgcag gcgtcctcgg cggcttcgtt ctttcgcagt
     4681 tcggaaccac gaccgaaaat ttggcgccga tcgcgcactg ggttgcgccc agcagatccg
     4741 cggcgattct gttggtcgcg ggggcggcgt tggcgctgat ccctttgatc tgggcgtcat
     4801 ttcgggtgct cgcgcgtccg catgcacgcc tgctcacagg cctgagcgcc gcaggccatg
     4861 ccctgctctt ggtcccgaat cgcagtgccc tcggggcgat gcttaccgtg gttccgattc
     4921 tgggggccgc tgcctggtcg gctaaccgtg tcaagccggc gaccttggaa tcccgcttgg
     4981 ctgtctcgag cctcgtgaca ccagcggtcg tgatcgcagc gcgtcagttc ctcttctacg
     5041 aagtcacgag cgctttctgg ggcacgatcc tcggaatcag tgccttggcc ctgttcatgc
     5101 tcggcgaaaa gatcagcgac acgagcctcg agcgcgtcgc gttcgcgccg gcgctgatgg
     5161 cgatcggcgc attcatagat gccgcacagg tcgtgaaccg ctggtcgttc tcgacgacgt
     5221 tgctctcgta cggcatcttc gccacgattc ccctgcttgc gttcgcgtgg cggagcgccc
     5281 gttcgcgggc cttctttgtt cgctgcgcca tgattctcaa cgcagcgacg gcgacgacga
     5341 ctctgctggt ggcgcctcgc ccctgggcgg cgctccaatg cctcgcgctc ggtctcgccc
     5401 tgctgagcta cggcttcatc cgtgaccgac gcgccgcgct ctactcgggg atatcgctgg
     5461 cggcattcgg cttcctcgtc gaggtcgccc acgcggtcga ggtcttccag ccgagcggct
     5521 ggctcgcgct agccggcttc ggcttcgttc tggtcggtct taccgcctgg ctggaacgcc
     5581 ggtcgagcgc tgccaaactg tcgcaggagc tggcgccagg gctgccacaa tgacagcaga
     5641 gcaccgcaat cttgctcact tttcctcgga agcattggag aaaatggtga tcgcgacgct
     5701 ggcatgagcc ctgcatagtt tgaaagcgtc atgcgccgaa caggcatcta tctcctagtc
     5761 gtcctttcgc tcgtggtcgg ggctgcggtg gtcacagacg ccatcgtggt caccgatcgc
     5821 gaacggatgg atcagttcat cgagtcggtg acaggccagg tgtccggatc gcgaatcgac
     5881 aacgcgctga gctatgccga cccctcgaag gtcacgatgg agctcgtgca tgacggacac
     5941 cgggcacgct acagcaaccg caacgcagcg aagctcaagt cggacgcgcg caaagccctc
     6001 gcctctctcg aaggatcgag gcttcacctg attcaggaga gcgtctcgct cgatggcgag
     6061 cgtgcgcgca tcgcgctccg tcttcgcacc gacgccggcc tcgccaacac cgtgttcgac
     6121 cttcgccgcg agaacgacgt atggttcttg cgccgcgtca tcgtcaacta ggcttcaccg
     6181 aagcggacgt cgcgcgcgct cacgccatgc acgcgtagca ctttgttgcg tgctcgggcg
     6241 ccacgaacga tttcaacgtc ggacttcgaa acaccgagcg cttttgcgag aagctttcgg
     6301 agtgcttcat tcgcagcgcc atccactgga gccgcggtca atgcgacttt gagcgcgcct
     6361 tcttgaacac caagcacgcg attgcggctc gcgcgcggtg caacgcgcac ctcgaacgtg
     6421 attgcgcctt cttgttctcg aatctctagc tcagtcatcg ctctggcaag cccacttgac
     6481 cccggaggcg aacccggcga gattccctgc gaatgcatcc ggtcttgttc agtattccca
     6541 cgccctgggg cgcgattccc atctactcct acggcgtgat gttgggaagc agcctgctct
     6601 tcgcttggta cttcatcatg tacatgggca agaagatcga aggctacaat cgagagctgc
     6661 tggcgagttg tttcacatgg accgctgtcg gcgcgatcat cggcgcccgc ttcctctaca
     6721 ttctgacgaa cttggactcg tacgagagcc tcggctcctg gttcgacctt cgctccggcg
     6781 gcttggttgc ttacggcggt tttctcggag gattcatcac cgcgtggtcc ttctggcgaa
     6841 tcaagaaaat cccgctgctt ccctttgcgg acctcgccgc cccgacactg gcgtccgggc
     6901 tcatgctcac gcgcgtcggc tgctacctct acggctgcga ctacgggcgc ccgctcctcg
     6961 agagtgcacc agcttggttg caaaaggcgg gcacttttcc gaagtgggac accgaggcct
     7021 acccggcgtt tgcctgcgac caggtgatca gcgggtcgcc ggcgtatcag caccatctcc
     7081 aagagtaccc ggaagcgatg atcgatcgac tgagctcgct agccgtgcat ccgacgcaga
     7141 tctacgaatc gctgttcggg cttctcttgt tcgtgttttc cgtctggctg ctgacgcatc
     7201 gcaagttccg cggacaagta ctggtaacgg tcgccggcat ttatgggctc tggcggttcc
     7261 tcatcgagta tctgcgcgac gatcccgagc gcggcttcgc ctttggtttc tctacatcgc
     7321 agctgatttc tctggcgatc attccggtct gcatcatcgc gtacattcac ctgaagaaaa
     7381 acgcggctga gaacggcgac atcgagctcc cagcttgggc gctcgagacc gctcgagggc
     7441 ctgcggtctc gccaagcgcg gctacatctg cagacggacg tccttcgaag tacaagccga
     7501 agaagatcaa gaagaagaaa aattagcctc cgctactcgt cggtgggctg cttcgctcga
     7561 ttgaatgctt cgagcacgcg cggcgatgtg cgcacggtgt cgagctcgag gttcggttgc
     7621 aatgccagcg ctgctcggaa cgatgcttct gcgaggtcgg ttcgttcgac cgcgacgtag
     7681 gcgaccgcga gctccttttg aatcgtcacc atctggctcg aggtgaggtt gttctttccg
     7741 agcagacgat tgccgagcgc aatcgcctcc gcgaacgcgg ccttctgccc ctgctcgatc
     7801 aggccgggaa gcgcactctc cacctcgtcc tgagccgcct tggtggctcc cccacccgcg
     7861 gcgcgcatga atgcggccga ggcttccgcg cgaccctctc cggccagtct cagatcgaac
     7921 agcggcacca gcacgatctg cccacgctcg atctcagccc ccgaattaaa gcgccgtagc
     7981 cgcttcagcg aaagagggct gttgggcatg tacatgccgg cgatcttcac cagggtctct
     8041 cccggaccga cgacgtgccg cagcgggtaa ggcaccagaa gctcggcgcc ttcgtcgggc
     8101 tcgactctgc gattaccgct gttggcttcg atcaaaatcg tcgctcgccc ttcgtctccg
     8161 tagagctttg cagcgatcga cttccaggtc tcgtccttgc cgacttgata gaaagtgacc
     8221 atcgggatga agagccaact cccctgaacc atggcgttgg agcccggccc cttcattcgg
     8281 ttcgcctcgc gaaggaccgc ctcgcgggtc gggtcgccgt agtagcgttg cgcgatcgaa
     8341 gcgagcgtgt cgccactctg aacggcgtgt gtgtacaccg cttgcgcatc cgcgatggaa
     8401 gcggccgccg caaacccgat cacggcggcg agcgttagaa agcggagccc gaccggcttc
     8461 acggcgtcgc ctccgggtca gccacgacgt tgatcgcctc ggacagcgtt gcgcggtctg
     8521 gcaccgtggt accggcttcg accacgacct cgtgctccac gggcgcgaag taaggattgc
     8581 tgtacttcac gtagtgtcga cccggtgtga gaggaatcga tcgcgccgaa ggcgtggtca
     8641 gcacgtgctg tccgtcgact tgcacgtgcg cccagggatc ggcgctcacc gtgaggtatc
     8701 ccgcatcggc aggattgagc gtgaccggcg cgtcgcccac tgcgggctcg gaccagcgcc
     8761 cgctcgcggc ttggaccgtg aggccggata cgaggatcag cacggccgcc gccgcttgcc
     8821 atttcgcaac attcttcaag aaaccgacgt ctcggcgacc gcttcgaagc acacgcggcg
     8881 ccacggacga gagcacctct tcggcctggt cacccgtcat caccccgatc tcgctgaggt
     8941 acatgaccag gcgcgcgttg tggctcatcg gcacgcgtgg cgcgagaaac tcggtaaggt
     9001 cgtcgatgag tgcttgggtc gtcgggtagc ggttggccgg cattttctgc aagcagcgcg
     9061 ccatgattcg ctctagcttg cgaggcacgt cgacgccgag cttcttcggc gactcgaagc
     9121 gatcgagccg gatcttctgc atcaccgttc gggagtcgtc ttcgacgaag ggctttcgcc
     9181 cagtgagcat ttggtagagc acgatgccca cggaaaaaat gtcgctgcga aagtcgagct
     9241 tgtccccgag gatctgctcg gggctcatgt agctcggcgt cccgagtccg gtgcctgtct
     9301 ccgtcagatc tccgaaacgc tcatgacgtg caatcccgaa gtccatcagc ttcacatcgc
     9361 cctgcacgga caccatgatg ttcgcgggct tgatgtcgcg gtgaatgatc ccgcggaagt
     9421 gtgcgtagtc gaggccgcgc accaattgca gcatgatgat cgcggctacg tccgcgggca
     9481 ggcgcggtga gcgttcgagc aagtcgtaga ggtcgatccc atcgacgtac tccatgatga
     9541 tgaacatgga gcggccgtct ttgatgaagt cgatgacgtg cagaatgttc tcgtgctgca
     9601 acgaggccat gaagtgtgcc tcgcgctcga accgctcggc gaactggctg tcgatggcga
     9661 tcgaaggctt gagcgctttg atggccaccc gccgattgag cggctcctga atcgcctcgt
     9721 agacgaccgc catgccccca ctcccgattt caccgagaac acggcacgtt ccgattcgct
     9781 caaccatcgc gatccatcac agtaggtggg gggttcggga gtgtcaacga tcgccaaaag
     9841 tttggcgaaa accgacactt agggcggaga ttcggcagct ggcgctggaa gcacgcagag
     9901 gccgcgttca acccagtcgg tgccaccgcg gccatcgcga attacgaagt tgaagcgcac
     9961 gagttgcccg tcttcctcca cccccgtggg cggagtccac tgcgtgaggg agtcgatcgc
    10021 cggcgtggtg atgaaggaga acgtttgcac caggccgcct ccgtcggtga gccaggtgat
    10081 ctggatctcc tcggtcaggg tcttgtcgtc aaccgtgtac gtctgaaggc tttcgcgggt
    10141 cacctgcagt cgaatgtccg ctgccgggcc gccggccacg ggatgcatgg cccgctcctc
    10201 gggggtgagc gaatcgagat cggccctgca acccgtacgg ggagcggtac gcgggacgcc
    10261 ttgatcgtaa gggggcggcc aagcggttcc ctcgtatcgg acttggtcga tttcggggtt
    10321 cagattggga tcgctcgcgt cggtctcgat cgggatctgc accacgacga agcgcccgtc
    10381 cggaggctcg tcctcacggc cgggctcggc acgcggtacg cagggttcta gatcgtcgga
    10441 ctctccggcg aggaagcgtt ggattgcatc ggtggagggc acgccgttca cgcagacgat
    10501 gccctggagc agcacggagg tcgcctgagc tgcctcgacc tccgattcgg aaggcacctg
    10561 gaaacgcatc accggaaacg caaagggatc tgccggtggc gtgcccacac agccttcaca
    10621 gggttgaatt atggccctcg gatctgcgca gatcggcgcc ccgatgagcg tgggcagggg
    10681 aaggcaagct gcgaacagcc actcgagggg ttcgggcgtg agcgcaggca ccccctcgat
    10741 gtccgatggg ggcgagccct tgtcgatgac ccgaagcgag acctcgaccc catcacccgg
    10801 ctccgggttg gcgcgaccgg gctctccatc gacatcgacc acggccgcta ccacgcgaag
    10861 gtcggtgact gcccatggcc caagaaagct gtcgctgcag gccacgatca gaccgagagc
    10921 gaacagcccc gtgatggccc aacggcggag ccgctcgttt cctgctttca tacctcgttc
    10981 atccctcatc agaactcccc ccggactccc agcgcgggaa tgattggaag cccgtagaga
    11041 ggtgtctgct gactgaagtc gtagttgtag atgatcccct cttggttctt cgcgttgtag
    11101 gcatttcgaa tgtcgaggaa gacggcaagg cgccaaccgt cgaggatcca cttcttctgg
    11161 acgcgcagat tgagctcatt gaagaggggg tttcggatcg agttcacgcg gccgtcgatc
    11221 ggaatgtaga cgtcgctcag agcgtcgtaa accgagccga tcaccggggt attgggatta
    11281 ccgctgacca ggcggaaggt gccgccgatc tcccagttgc ggcggaaatt gtagacgaag
    11341 gcaagggtga ggatgtgcgt ctgatcgaaa tcaaagagcc tccaaggctc gagctcgccg
    11401 tccttccgtt cgcttcgtga aagcgtgtag gagaggtagc cgaagtactg ccgaccggtg
    11461 gcgggtcgaa ggttgatgct gaactccccg ccgtagatcc gtccgatacc tccggtgacg
    11521 tagaagggcg cttcgccgtt gggggtcgcg atcgtcctgt cccagaggta cttgtagaac
    11581 ccttctaccc cgaatcgaac gccgggaaag gcatcgtatt cccagcccgc accaacgtgg
    11641 accgcctgga tgggatccag attcgggttt ccgatggtca cgtccgattc ctggaactcg
    11701 gggggctggc tgaagagacc gacgccgagc ttgaccctca tgtccttcgc cacggtgaag
    11761 atcgtggtga gccgagggtc gaccgtgaac gcgctgatgt cgccgaagta gtccaagcgc
    11821 agaccgagaa tgagccgggt gagctttccg agctccatgt ccgattcgag atagacgccc
    11881 gggcgataca ccgtggcgct cacatcggcg aagacctgcg gttgaccggc cagcgagcct
    11941 tgggcgctgc ccgcgccttc ctgcgatccc ggctgcggtc cgatgtaggt cagattcaca
    12001 ggagcaacga acacgtccag gccgctgatc aatcgaatct tgtcggtgat ctgataccgc
    12061 cattcggcgc ggccgtaggt ttggatgaac gagcctcgga gctgaaacgc gtcacccagc
    12121 gagaggttga tcttggttgg accgacgttg aacgcgagat cctgctcgac cttgtcgccg
    12181 acccgacgcg cccagtcgat ctggttgttg tagaacgagc ttccgagcgc cgtgttgcct
    12241 cgaatggcag ggtcgtcgct ggagggctct tctagaaaga gctcgagacg gtcgttcgac
    12301 ccatacgccg agatccgcac gcggtctcgc ttgctgggca agaaatttaa tttgaattgg
    12361 tagtcgatgt agacgggcgc ctgccgtaca ccgatgtcgt cggggagcac cgccgggagg
    12421 atgaggtcga tgaagcttcg ccgcagacca gcggcgatct ggatcttgtc ggtgatcgga
    12481 gcctcgacgg tgaagaacgt atcgatcacc gagatctcgg cgacaccgtg aacgcgatcg
    12541 cgagccgggt ccttggtagt cacctcgagg atgccgccgg tacgcctgcc gtaccgcacg
    12601 gagaagttac ccgggtagaa gtcgatcttc tcgagcgcat aggaattgta gacgctggtg
    12661 aggccgccga aatggtagat cagaggcacg gggacgccgt cgatgaacga ctcgctgtcc
    12721 tggggcgcgg agccgcggac gattaataga ccgagaccaa agggaggcct tgcaacaccg
    12781 ggaaggagct ccacggcacg aagcgcatca cctcggcttc caggaatccg ggtcagctct
    12841 tctcggtcga tggttcgctt gacgacttcg cgcggcggcg cctcgacgac cgcggtttcg
    12901 ccgaagcccc ggtacgatgc cttcttctgc gcgccgagcc gatagatgac ctgcgtgacc
    12961 tggcccgcgg cgagctcctc ttcggccttc aagtcgccaa actcgccgcc gaggatggtc
    13021 acttcgtagg tgcccggttc gaggtcggtg acgacgaagg ttccgtccgg gcccgcaacg
    13081 agctcacgaa cgagctcgtt ccgttcgttg agaatctcga tcgagaccga gccggctgcg
    13141 ctctcgtttt cggctagaag taccgtaccc gaaagccagg ccgtggtgat ctcctcgggc
    13201 ggctccttca gctcgaagat gtaccgatag cgaatccgcg cggcgatcgg ctcccaatcc
    13261 ttgcgcgcgg gctcgaacac gaactggcgg gccgcggcga gagccgcctc gtcgaatgcc
    13321 tcccccgcgg acctggccac gctcacttcc gtgaccaggc catcctttcc aaccacgatg
    13381 tcgagctcga ccgcggcttc gacaggctct tcaccctccg tcttcggata cgccgcctcc
    13441 acgaagcgaa ccagcttcgg aggcgtgacc agcggcttct tttcttcgtt cggcgcgagc
    13501 tgaaagctcg tctgcgcata cgtcgcgctc gagacgatgc ttacgagcag gaacgcagcc
    13561 agaaagcgaa gcacggcctc agcatacgga ccacatcggg cacagacaaa cagagcggag
    13621 ggcggtcgat ttggggcctc aaagcgcaga ggcggaacct gaaccatccg caatccagtt
    13681 aaggtgcccc ggttgcctgc tcagacaacc ggggctgggt gttggatatg cgttactagc
    13741 taatgttggt ggaggtggga gacgcggcta gtaggtcggg aagatacacg gtcgtgagtc
    13801 gatgttcggt gccgagatcg tgaacacgcg gtgacgacgg ttaaaacgaa accgccgctc
    13861 ccagattggt tgcggcggtc gcgcttgtgg gtcggcgcta ggcgcgccgc gtgtcgctat
    13921 tagagccccg cggcgccttc cggataggct gtccagtccg aggtccaatc ggtgcccgag
    13981 ggaccgccaa aggcgccgag gcagttcgcg ttcgcgaggc tgtagtccgg tgcggggtta
    14041 ccgcccgtgg cggccgtgct gaagtcaccc aattcagggt cttcttcgag attgccctgg
    14101 gttggcatgt tggtgttctc ggggaagtag ttagcctcat ccgtgaagtc gccgctcccg
    14161 ctgtagtcat tgcagtcttc cgcgcctatt gcattgccgg gattctccgg gtcttcgatg
    14221 caatcaacgt ctgccgggaa gttgggggtg ttgtcgttga agcagctgtt tgtgacgacg
    14281 attccatcag gccaaccgcc agcgacgctc caagccccgt tgcgcatgtc gtagcccgcg
    14341 ccaccaaagt caacgacgac caggccatcg aggatgcccc aggtgccaac tcgtgtgacg
    14401 acgccggagc cttccggccg accatcgccg atgaccgtcg cgttgcgaac ctgcggagcc
    14461 gaacgcggct gcacgttgtt gttcccggag ccgtagttgt cggcttcgat tccgttcggg
    14521 ctcgaactgg tcgacgagaa atggtgtacg atcacgttgt ctgcggtgcc acgccaaccc
    14581 tgatcccagt cgagaccatc gtctccgggg ccggtgacaa tgaggtggtc gattggggct
    14641 gtacccccga agaactcgac gccgtcatcg aggccgcggt gtacctggat gtagctcaga
    14701 acggtctggt cgccgca
//
LOCUS       DASRZF010000072         2362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2120040, whole genome shotgun sequence.
ACCESSION   DASRZF010000072 DASRZF010000000
VERSION     DASRZF010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2120040"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VFG22_03185"
     CDS             complement(<1..297)
                     /locus_tag="VFG22_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6415275.1"
                     /translation="MRRVLVVDDEENIRLVLRTLLKKHEYQVETAESAESALEQLERF
                     DPDFVLADVRMSGMTGIELCAELKARSSLATVILMSAYGSVDLAIDAMKAGAYDY"
     gene            456..2312
                     /locus_tag="VFG22_03190"
     CDS             456..2312
                     /locus_tag="VFG22_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415276.1"
                     /translation="MKIRFAFLLVMVGLIATVGSCKGQPLNALNPCTINGVVQNVPVN
                     PQRALDLLIVIDDSPSMLQEQAKLAEQVPRLVSLLLTGGEDTDGSKPVGDFPAVESLH
                     VGIITPDLGYSTVPATNFTAGPNPDDDFNPTDKCMRNNGNGNAGFMQVEGLSGSSRVV
                     CEAQTPPEGTLYLNYPEPGFTRIDFIDDVECVTGQDDGCGFEQQLESILASDRNTANG
                     GFNREGALLAVILITDEDDCSTTDPRVFDVEPRASNPFQGPFTSTDELQFNLRCFAHS
                     DALQSIQRYVDGVAALKDDPSQVVFAAITGIPEDSALDRANFNSDADRYAAILADGAM
                     TEVPNPAEADTQGQTLTPACVASDGSGAAAPGRRIVETMQGLAAGNTGVGTVVESICA
                     ADYAPALNAIVDRIAAALRQLCLPRPLNRNSQNLVGCEVREVQPEGATCANAGRGREP
                     EAVSNEDGREVCRVTQLPSDASAGEPDGLGWFYDDFTPETVSACAFNPEQQRVTFTDG
                     SAPETGARIRFECLQAAPPTTEDIGWPCAIETDCDPRPENCLDGESIESCETRILKER
                     YDRDNLVLSCDSESNTCQLACESNVQCPGGYACYDESDDGNSYCVNPTCTLN"
BASE COUNT          473 a          763 c          709 g          417 t
ORIGIN      
        1 gtagtcgtac gccccggctt tcatggcgtc gatcgcgagg tccaccgacc cgtaagcgct
       61 catcaggatc acggtcgcca gggacgagcg ggccttgagc tccgcgcaaa gctcgattcc
      121 cgtcattccg ctcatgcgaa cgtccgcgag cacgaaatcc gggtcgaagc gctcgagttg
      181 ctccagggcc gattccgcgc tctcagcggt ttcgacctgg tactcgtgct tcttcagcag
      241 cgtgcggagc accagccgaa tgttttcttc gtcatctacg accaacacac gccgcatcaa
      301 ttccacccga tactttctgc atagcagaat ctccgactaa gaaaaaattt cacaattctc
      361 gggcgatagg gcaaggcttg ctgtaaagtt cttgctcgat tggctttact gcgccccagt
      421 agctatgatt ttgctgtcgg gtagggggag aagacatgaa gatccgcttt gcttttttgc
      481 tcgtgatggt tgggctcatt gcgaccgtgg gtagttgtaa ggggcagcca ctgaacgccc
      541 tcaatccatg caccatcaat ggcgtcgttc agaacgtgcc ggtgaacccg cagcgcgccc
      601 tggacttgct gatcgtcatc gacgactcgc cttcgatgct gcaagagcag gccaagcttg
      661 ccgagcaggt gcctcggctc gtgagcttgt tgctcaccgg tggagaagac accgacggct
      721 cgaagccggt cggagacttc cccgcagtcg agagccttca cgtcgggatc atcacgccgg
      781 acctcggata ctcgaccgtg cctgcgacca acttcacggc gggcccaaac cccgatgacg
      841 acttcaaccc caccgacaag tgcatgcgga acaacggcaa cggcaacgcc ggtttcatgc
      901 aagtggaagg tctcagcggg tcatcgcgtg tggtttgcga agcgcagaca ccgcctgaag
      961 ggacgctcta cctcaactac ccggagcctg ggttcacgcg gatcgacttc atcgacgacg
     1021 tcgaatgcgt gaccggacag gacgacggtt gcggcttcga gcagcaactc gagagcatct
     1081 tggcttcaga ccgcaacacc gccaatggtg gtttcaaccg cgagggcgcc ttgctggctg
     1141 tcattctcat cacggatgag gacgactgct cgaccaccga cccgcgtgtc ttcgacgtcg
     1201 agccacgcgc aagcaatccg ttccaggggc cgttcacctc gaccgacgag ctgcagttca
     1261 acctgcgctg tttcgcgcac agcgatgccc tgcagagcat tcagcgatac gtcgatggcg
     1321 tcgccgcgct caaggacgac ccgagccagg tcgtcttcgc agcgatcacc ggaattcccg
     1381 aggatagcgc gctcgaccga gcgaatttca atagcgacgc cgaccggtac gccgcgattc
     1441 tcgctgacgg cgccatgacg gaggtgccga atcccgccga ggctgacacc cagggacaga
     1501 cgctcacgcc cgcttgcgtt gcaagcgatg gatctggcgc agcagcacct ggacgccgaa
     1561 tcgtcgagac gatgcagggc ctagccgcgg gtaacaccgg agtcggcacc gtcgtcgaat
     1621 cgatctgcgc cgcagactat gcgcccgcgc tcaatgcgat cgtcgaccgc atcgctgccg
     1681 cgcttcgcca gctttgtctc ccgcgaccgc tcaaccgaaa ctcccagaac ttggtgggtt
     1741 gcgaagtgcg tgaggtgcag ccagagggtg cgacctgcgc gaacgccggc cggggccgtg
     1801 agcccgaggc cgtcagcaat gaagacggtc gcgaggtctg ccgagtgacg caacttccga
     1861 gcgacgcctc ggccggtgag ccggacggcc tcggttggtt ctacgacgac ttcacgccgg
     1921 agacggtgtc ggcatgcgcg ttcaatccgg aacagcaacg cgtcaccttc acggacgggt
     1981 cagctcccga gaccggcgcg cgcattcgct tcgagtgctt gcaggccgct ccgccgacga
     2041 ccgaagacat cggatggccc tgtgccatcg aaaccgactg cgacccgagg ccggagaact
     2101 gtctcgacgg cgaatccatc gagagctgcg agacgcgaat cctcaaggag cgctacgacc
     2161 gcgacaacct ggtgctcagc tgcgacagcg agtcgaacac ctgccaactc gcgtgcgaaa
     2221 gcaacgtcca atgcccgggt ggctacgcct gctacgacga aagcgacgac ggcaacagct
     2281 actgcgtgaa ccccacctgc accctaaact gacggggagc acgggcacgg ttcgctggcg
     2341 ctgacactgg cgctagcgct gg
//
LOCUS       DASRZF010000073        11894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3837465, whole genome shotgun sequence.
ACCESSION   DASRZF010000073 DASRZF010000000
VERSION     DASRZF010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 11894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11894
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3837465"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..537
                     /gene="moaC"
                     /locus_tag="VFG22_03195"
     CDS             49..537
                     /gene="moaC"
                     /locus_tag="VFG22_03195"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HET6415277.1"
                     /translation="MNENEELTHLDRDGRARMVDVGEKDATQREARARATLRMNPETR
                     RVLLTGQVPKGDALAAARIAGIQAAKRTSELIPLCHPLAITGCDVVFEPADDEERFVI
                     ETSVRCTGRTGVEMEALTAAAVAALTLYDMLKAIDREMAIEEIVLVEKQGGRSGHYRR
                     GR"
     gene            complement(534..2045)
                     /locus_tag="VFG22_03200"
     CDS             complement(534..2045)
                     /locus_tag="VFG22_03200"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HET6415278.1"
                     /translation="MDSSEDARRAGRGLVSIAGAKAFFIVSSYAVQLLLPRIFGEAKE
                     FGLYAATMSGVAILTNVLIVATIQSVSKFVSEDEGRAEVTLRQGLRVQTLVGGLLAVG
                     LFLMAPMIAHLLLDEQLTRMLRVASVVVFAYALYAAVVGSLNGRHLFAKQARLDMTFS
                     TLRTAGILAGAALGFGAIGAVAGFAAASAGILSISLIWIGFGRRGAPIPLRRWLGFMA
                     PIYAYQICLNGILMIDIQVLKRTATELAFASGVTGQAAVDLANQYVGYYRAAQTFAFV
                     PYQLIISMTFIVFPMISKATSIGNQEAARATIQHAMRLSLLLLLLVAAPTSGAAEGVM
                     RLAYPSEYLAGTSALAILVFGVAAFALFAVAATAISGAGKPSIAALIAAVSLLTVFVA
                     NRVLVLRAGLGMEALRAAATGTTIGMVVALLLSAWVTYKFFGVFIPVLTWLRAALAGL
                     SGYWLAAAVPHGSAWMTIVALLLGFVSAVVVLVITRELGNDDWRALRRVTRRD"
     gene            2171..3172
                     /locus_tag="VFG22_03205"
     CDS             2171..3172
                     /locus_tag="VFG22_03205"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HET6415279.1"
                     /translation="MSAAGVLGAVMLVGAGSYYSIGAGRWSWFDCFYMTVITLSTVGF
                     AETLEGMNEIPEARAVTLTLIILGSGTLLYFISSLTALIVEGDLQGILRRRSMERAID
                     ALKQHVILCGIGTTGWHIATELRAVGVPFVVIDSDRERLDELNAEFKDTLLYVLGDAT
                     DDHVLDVAGVERARGVISALNDDKANLFVTISARALNPTARIVAKSIEPSTERKLRRA
                     GADAVVAPNYIGGVRLFSEMISPKTVAFLDRIVQFGGGISVGLEAIDIPPTSPIVGKR
                     LAETDIRRAGALVVAVHRSDGDYVYNPGSEHVLEAGDSLIVLAESQDAAALRDTVVG"
     gene            3312..5219
                     /gene="dnaK"
                     /locus_tag="VFG22_03210"
     CDS             3312..5219
                     /gene="dnaK"
                     /locus_tag="VFG22_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HET6415280.1"
                     /translation="MGKIIGIDLGTTNSVVAVMEGKDPKVIVNEEGDRITPSVVAWDD
                     QGEVLVGQIAKRQSITNPEGTVYSTKRFMGRRFDELGDDATRMPYKVVQRDNGDAGFE
                     VRGKTLSPPEVSAQVLRKLKKAAEDYLGQPVTEAVITVPAYFNDSQRQATKDAGKIAG
                     LDVKRIVNEPTAAALAYGLDKTEDHLIAVYDFGGGTFDISILEVGDKVVDVVSTNGDT
                     HLGGDDIDQRIIEYLVSEFKKDTGIDVGKDKMVLQRLKDAAEKAKIELSTKLETTVNL
                     PFLTADAAGPKHLNIRLTRAKLESMIDDLVQRTLEPCKKALSDAGKTAADIAEVVLVG
                     GSTRMPVVQETVSKFFGKEPHKGVNPDEVVALGAAVQAGVLSGDVQDMVLLDVTPLSL
                     GVETLGGVMTALIQRNTTIPTRKEEVFSTAEDSQTKVEIHVLQGERAEARNNRTLGRF
                     HLEGIMPAPRGIPKIKVIFDIDANGILSVTAKDEATGKDQKVTITSNSGLSDAEIDKM
                     VEDAAAHEEDDRKRREQIETRNKADQLAYGVEKALAEVKDKLPADKVSEVEGKVKSLR
                     DAIEANDAAAMTKLSTALEAAMKEIAEVAYSTATGAPEGAPGGAASSSASDDAGKGDD
                     DVIDAEFEEGD"
     gene            5352..7946
                     /locus_tag="VFG22_03215"
     CDS             5352..7946
                     /locus_tag="VFG22_03215"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HET6415281.1"
                     /translation="MPALDIPFFGTLVLCLILISASYTMAVAVGAGQGRPHLLPSARW
                     GTYATCALVLVAVCVLAYAFQVHDFRIRYVARYSDRSMPWWYLIASLWGGQDGSLLWW
                     AFLLSGYTFFATRWLRGRYTELQPWILATLMSILIFFAVLMLFAANPFATYIRTTPGD
                     GEGLNPLLQNYWMAIHPPSLYMGFVGWSVPFAILIAALITGRLGNEWVRAARLWAMIA
                     WTFLSLGLLLGCLWSYEELGWGGYWAWDPVENASFLPWLVGTAYLHSVLIQERRHMMK
                     VWNVFLLALTFFMTIFGTFLTRSGLIASVHSFARSDIGQYFVWYMGFLLIGIAALMIW
                     RIPKLKADNEIESLVSREFAFLLNNWVLLGMMVFVLIATTFPLLSQWIRGEEVTVGPG
                     FYNKWMVPLGIVLLFLAGLGPLVAWRKATGSKLLRAMAVPVGVGVAVAVLQFLFGARF
                     GYPPIVEPTEIYDTATGTALAWMSAIAPVVSFATCAFVLASVGQEFWRGVRVRMKKGE
                     NIGTALTRLVSKGRRRYGGYIVHVGVVFMYIGFTGAAYDVEQESALRPGDTMSIGQHT
                     VRYDQPRMEADPNKRMVFTDMTLIDESGNEDGAVAPAKFIYRTHPQMPTTEVAIRSRL
                     AEDVYVIMSTVDPSTRRGTFRVIIRPLVAWIWLGGIVLLLGAILGMWPTGKELLREEV
                     ARPRRFRFSPATAVVGLAIIIATVMAWAGVAAAQNNSSSSLHAGSVVINDPVERQLFG
                     RLLCQCGDCARLPLDTCACGWADEKRIEVREQLAAGVPPTKIQESYRAQYGAKALAIP
                     SDEGLDRALWAVPLGAMVLAVGFIVVLGRRWQRRGVVLATSGASTAGAEFEYDARLDE
                     ELDRLEDS"
     gene            7946..8311
                     /locus_tag="VFG22_03220"
     CDS             7946..8311
                     /locus_tag="VFG22_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415282.1"
                     /translation="MSELLPWIFVITASVALVGGLVSLWLSLSMALSDEALGGARAQL
                     ITDARRALLAEKEALLQEIRDVSFEHDADKLSDYDFNEINEKLRAQARHVLHELDAGV
                     ELYRDEAEALIAARLADEV"
     gene            8311..9501
                     /locus_tag="VFG22_03225"
     CDS             8311..9501
                     /locus_tag="VFG22_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415283.1"
                     /translation="MRIAVLIGLLLWVPSIASAQPDQAVFDRVTGNVPDIVTSRVDPS
                     LPTGTIVVEVVDRDGGPAPKQAVQLGLMEQSGGRDSKACVTDEAGTCSFAELSTDAKD
                     SYRVNVPYQGARYSSTPFRLDTSAGQRVRVTRLPTTTDDERIFQLLGRTIIEFRDDRA
                     HIQQEARLLNLGDSTYVFPEGGMRIRLPDGFKAFDSMAVMTDQLLSATDEGLEISGSI
                     PPGRVELMWAYDVPAGGSSLSIEQAVPFATMEYQVISDYVDGLSMVVEGFQVARVHEG
                     GDRRFLVSGLMRRPGDPPIDPLRIHISGIPGGGPLPYLASAIAAIFVFLGIWLLFRPL
                     DQSEILAKVREERRLELLDEIVLLESQRKTESIGPGFYENRRRELTDELAIVLRMQSE
                     GTGH"
     gene            9561..10001
                     /gene="ssb"
                     /locus_tag="VFG22_03230"
     CDS             9561..10001
                     /gene="ssb"
                     /locus_tag="VFG22_03230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HET6415284.1"
                     /translation="MAEGLNRVILIGNLGADPELKFTQNGQGVLRLRLATTESYVNRA
                     GERQQRTDWHTVTVWGKRGEALNNILSKGRSICVEGRIQYRQWEDKDGNKRNSTEIVA
                     TNIVLVGSGRGRDSGAPGDFGGGDTGGGGSSPPTDDFGDDDIPF"
     gene            complement(10067..10564)
                     /locus_tag="VFG22_03235"
     CDS             complement(10067..10564)
                     /locus_tag="VFG22_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415285.1"
                     /translation="MARNVLFFLGVGSIGWALLSLFAGSILPEWMGLSGVQHGQLYIE
                     SIALNVGLWGALGVFALLGALFQRLRLSASLAFLVCATGIASGRVLAMVQGAKPDPYI
                     VLALALEVAIVVSASAAYVSEKARIAREAKELKRAEKAALEAALAAEHALPVEAPAAS
                     PVQQP"
     gene            10619..11377
                     /locus_tag="VFG22_03240"
     CDS             10619..11377
                     /locus_tag="VFG22_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HET6415286.1"
                     /translation="MPKVRADVLLTQRGLVPSRARGQALILAGKVFSGDRRVEKSGEA
                     LDEDAPIEVREPDHPYVSRGGVKLDGALDLFAYDPANKTAADFGASTGGFTDCLLRRG
                     ASKVFAIDVGYGQLHNKLRQDERVVAMERCNARHLKAGDLPEPIELIVIDASFIGLEK
                     LLPAAHALLPVGGDVLALVKPQFQVGRDKVGKGGVVRDAALRREAIATLVTEAERLGF
                     ELVAEADSVITGPKGNQETFIWLRRLAAAPAPRF"
     gene            complement(11331..>11894)
                     /locus_tag="VFG22_03245"
     CDS             complement(11331..>11894)
                     /locus_tag="VFG22_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415287.1"
                     /translation="PSLGLGCDHEIEASVVAKHSWPYPAEYRNLRWGNQYARVTLKLK
                     PNHASFITATVSWKAGDLIEVEDSDVLILKPRRLVAKRDLFVTRKVWDQGIQVERRFL
                     AVPKGEVGSFLFYNSKGLCMIGSEAGTAWTPCTLDDAFEGLSAQHPNACEQVWWAKVG
                     KSKADRGWMPVDLRIVERVPPPSDAAR"
BASE COUNT         2311 a         3623 c         3731 g         2229 t
ORIGIN      
        1 agctaccatc ccgccgcggc ccaaccacga cgggacgctc gccgattgat gaacgagaac
       61 gaggagctca cacatttgga tcgcgacggt cgggcccgga tggtcgacgt cggggagaag
      121 gacgcgaccc aacgcgaagc ccgtgcgcgt gccaccctcc gcatgaatcc agaaacgcgg
      181 cgcgttctcc tgactggtca ggtccccaag ggcgacgccc tcgctgccgc gcgcattgcg
      241 ggtatccagg cagccaagcg gacctccgag ctcattccac tttgtcatcc gctcgccatc
      301 accggttgcg acgtggtttt cgagccagcc gacgatgagg agcgattcgt catcgagaca
      361 tcggttcggt gcacgggccg caccggggtc gagatggaag cactgaccgc cgcggccgtc
      421 gctgccctca cactctacga catgctgaag gcgatcgatc gcgagatggc gatcgaagag
      481 atcgtgttgg tggaaaagca gggcgggcga agcggtcact atcgccgcgg acgctagtcc
      541 cggcgcgtta cgcgccgaag cgctcgccag tcgtcgttcc cgagctcgcg ggtaatcacc
      601 aaaacgacga cggcgctaac gaagccgaga agcaaggcaa cgatggtcat ccatgccgaa
      661 ccgtgtggaa cggccgctgc gagccagtac ccgctcagcc ccgccaatgc tgcacgcaac
      721 caggtcagca ctgggatgaa cactccgaag aacttgtagg tcacccacgc cgagagaagc
      781 agcgcgacga ccattccaat cgtggtccct gtggccgcgg ctcggagagc ctccattccg
      841 aggcccgccc ggagcaccaa cacccggttc gcgacgaaga ccgtcagcaa tgaaacagcg
      901 gcgatgagag ccgcgatgga aggcttgccc gctccgctga tcgccgtggc cgctactgcg
      961 aaaagcgcga atgccgcgac gccgaagacg agaatcgcca gggcggaggt cccggccagg
     1021 tactccgaag gataggcgag acgcatcacg ccttcagcag cgccggaggt gggcgccgcg
     1081 accagcaaca gaagcagtag cgacaagcgc atcgcgtgtt gaatggtcgc ccgagcggcc
     1141 tcctggtttc cgatgctcgt cgccttactg atcatcggga atacgatgaa ggtcatcgaa
     1201 atgatcaact gatagggaac gaacgcaaac gtctgggccg cacggtagta acccacgtac
     1261 tgattggcga ggtcgactgc ggcttggccg gtcacgccgc ttgcgaacgc cagctctgtc
     1321 gcggtgcgct tgaggacctg aatgtcgatc atgagaatac cgttcaggca gatctggtac
     1381 gcgtaaattg gagccatgaa accgagccag cgtcggagcg gaataggagc gcctcgtcgg
     1441 ccgaagccga tccaaatcag gcttatcgac aggatgccag cggatgccgc ggcaaaaccg
     1501 gccaccgcgc caatcgcccc gaacccgagg gcagcccccg cgaggattcc cgcggttcgc
     1561 agtgtcgaga aggtcatgtc gagtcgggcc tgttttgcga acaagtgacg cccgttgagg
     1621 ctgccgacca ccgcggcata gagggcgtag gcaaacacca ccacggaggc gactcgaagc
     1681 atgcgggtca gctgctcgtc caggagcagg tgtgcgatca tcggggccat gaggaagagc
     1741 ccgacggcaa gcagacctcc gaccaaagtt tgaactctaa gcccttgtct gagcgtgacc
     1801 tcggcgcggc cctcgtcttc gctgacgaac ttcgaaacag attggatcgt cgcgacgatc
     1861 aagacgttgg tgaggattgc gacaccactc atcgtcgccg cgtacagccc gaactccttc
     1921 gcctccccga agatcctcgg aagcagaagt tgcaccgcgt agctgctgac gatgaagaac
     1981 gctttggcgc ccgcaatcga caccaagcca cgccccgccc ggcgcgcgtc ttcggaggaa
     2041 tccaccggtg ccggttgtgc agtcccggct cggcctggtc aagggttgac ttggatcggt
     2101 gcatgacgcc aactacgccc cagtgaagtt gtggttcttc aagtcgagac gcggggctta
     2161 cggccgcttg atgtcggcag caggggtcct cggggcggtc atgctcgtag gtgccggctc
     2221 gtactacagt attggggccg gtcgctggtc ttggttcgac tgcttctaca tgacggtcat
     2281 caccctctcg accgtgggct tcgccgagac gctagagggg atgaacgaaa tccccgaggc
     2341 gcgcgccgtg accttgaccc tgatcatcct cggcagcggc acactgcttt atttcatttc
     2401 gagtctcacc gctttgattg tagaaggtga cttgcaagga attctgaggc gtagaagtat
     2461 ggaaagggca atcgacgcgc tgaaacagca cgtgatcctc tgcggcatcg gcaccacggg
     2521 ctggcacatc gccaccgagt tgagggctgt cggcgtgcct tttgtggtca tcgacagcga
     2581 tcgcgaacgg ctcgatgagc taaacgcgga gttcaaagac accttgctct acgtgcttgg
     2641 ggatgcgacc gacgaccacg ttctcgacgt cgccggcgtc gagcgcgcac ggggagtgat
     2701 ttccgcactg aacgatgata aggccaacct tttcgtcaca attagtgccc gagccctcaa
     2761 cccgaccgca cggatcgtgg ccaagtcgat cgagccttcc accgaacgga agctgcggcg
     2821 tgccggcgcg gatgccgtgg tcgcgcccaa ttacattggc ggcgtgcgct tgttcagcga
     2881 gatgatcagc cccaaaacgg tcgcgttcct ggacaggata gtccagtttg gcggcggcat
     2941 cagcgtgggc ctcgaggcga tcgacatccc tccgacgagc ccgatcgtcg gaaaacggct
     3001 cgccgaaacc gatattcggc gggccggcgc gctggtcgtc gcggtccaca ggtcggacgg
     3061 cgactacgtc tacaaccctg gaagtgagca cgtgctcgag gccggggact cgttgatcgt
     3121 ccttgccgag tctcaggacg ccgctgcgtt gcgcgatacc gtagtcggtt gacggcgccc
     3181 ggtgccccca ctctccaggc gttgatttcc gtctcgattt cggcgttcga aggtccttga
     3241 cgagcatctt cgggtggtta ggttggcgaa actccggcgg cagctcgggg acaagaagga
     3301 gataagagaa aatgggaaaa attatcggca tcgacctcgg gacgaccaac tccgtcgtcg
     3361 cggtgatgga gggcaaggac ccgaaggtca tcgtcaatga ggagggtgac cggatcacac
     3421 cgtcggtagt ggcttgggat gaccagggcg aagtcctcgt gggtcagatc gcgaagcggc
     3481 agtccatcac caaccctgaa ggcaccgttt attcgaccaa gcgtttcatg ggtcgccgtt
     3541 tcgacgagct cggcgatgac gcgacgcgca tgccgtacaa ggtcgtgcag cgcgacaatg
     3601 gcgacgcggg ctttgaagtt cgaggcaaga ccctctcgcc ccccgaggtc tccgcgcaag
     3661 tgctccgcaa gctgaagaag gccgcggagg attacctcgg tcaacccgtg accgaggcgg
     3721 tcatcaccgt gccggcatat ttcaatgact cccaacgtca ggccacgaag gacgccggaa
     3781 agatcgcggg tctcgacgtc aagcggatcg tcaacgagcc gactgccgcc gcgctcgcgt
     3841 atggcctcga caagaccgag gaccatctca tcgccgttta cgacttcggc ggcggcacgt
     3901 tcgacatttc gattctcgag gtgggcgaca aggtcgtcga cgtggtcagc accaacgggg
     3961 acacccacct cggcggcgac gacatcgatc agcgcatcat cgagtacctc gtgtccgagt
     4021 tcaagaagga caccggaatc gacgtcggca aggacaagat ggtcttgcag cgcctcaagg
     4081 acgccgccga gaaggcgaag atcgagctga gcacgaagct cgaaaccacc gtgaaccttc
     4141 cgttcctcac tgcggacgcc gcgggcccga agcatctcaa cattcggctt acacgcgcga
     4201 agctcgagtc gatgatcgac gatctggttc agcgcaccct cgagccttgc aagaaggcgc
     4261 tttccgatgc cggaaagact gcagcggaca tcgccgaggt agttctggtc ggaggctcca
     4321 cccgtatgcc cgtcgtccaa gaaacggtga gcaagttctt cggcaaggag ccccacaagg
     4381 gcgtcaaccc ggacgaggtc gttgcgcttg gcgctgcggt tcaggcgggt gtgctctcgg
     4441 gcgatgtgca ggacatggtc ttgctggacg tcactcccct gagcttgggc gtggagacgc
     4501 tgggcggcgt catgaccgct ctcattcagc gaaacaccac catcccgacc cgcaaggaag
     4561 aggtgttctc caccgcagag gacagccaga ccaaggtcga gatccacgtt ctccagggcg
     4621 aacgtgcaga agcaagaaac aaccgcacgc tgggtcgttt tcatctcgag ggaatcatgc
     4681 ccgccccgcg cggcatcccg aagatcaagg tcatcttcga catcgacgcc aatggcattc
     4741 tctcggtcac agcgaaggac gaggctactg gcaaggacca gaaggtgacg atcacgtcca
     4801 acagcggtct ttccgacgcc gagatcgaca agatggtcga ggatgcggct gctcacgagg
     4861 aggacgaccg caagcgtcgc gagcagatcg aaacccgcaa caaggccgac caactcgcct
     4921 acggcgtgga gaaggcgctc gcggaagtga aggacaagct ccccgcggac aaggtgtccg
     4981 aggtcgaagg caaggtgaag tctcttcgcg atgccatcga agccaacgat gccgcggcga
     5041 tgaccaaact gagcacggcg ctcgaggcgg ccatgaaaga gatagccgag gtggcctata
     5101 gcactgccac tggagcgccc gagggtgcgc caggcggcgc ggcttccagc tcggcctcgg
     5161 acgacgcggg taagggtgac gatgacgtca tcgacgctga attcgaggaa ggcgactaac
     5221 tccggtctaa caacccccga aaagccccgt ccggcccagc gccgcgcggg gcttttcatt
     5281 gtgcttgccc gaatccgatc aacccctatg tattggggac ggcagcccgg ggattcgggg
     5341 gcccgattac tatgccagca ctcgacatcc cttttttcgg cacactcgtg ttgtgtctaa
     5401 ttctcatcag cgcctcgtac acgatggcag tcgcggtggg cgcagggcag gggcgcccgc
     5461 acctcttgcc atcggcccgc tggggaacgt atgcgacctg cgcgctcgtc ctcgtggcgg
     5521 tctgcgttct ggcctatgcg tttcaggtcc acgacttccg gattcggtac gtggcccgtt
     5581 acagcgaccg gtcgatgccc tggtggtatc tgatcgcgtc tctttggggc ggacaggacg
     5641 gctcgctgct ctggtgggca ttcctcctct ccgggtacac gttctttgca acccgctggc
     5701 ttcgcggccg gtacacggag ctccagcctt ggatcctcgc gacgctgatg agcatcctga
     5761 tcttcttcgc ggtcttgatg ctcttcgcgg ccaatccatt tgcgacctac atccggacca
     5821 cgcctggcga cggcgaaggc ctcaacccgc tgctccagaa ctactggatg gcgatccacc
     5881 cgccgagcct gtacatgggg ttcgttggct ggtcggtgcc tttcgcgatt ctaatcgcag
     5941 cgctcatcac cggccgcctc ggcaatgagt gggttcgcgc ggcgcgcctc tgggcgatga
     6001 tcgcctggac cttcttgtca ctcggcttgc tcctcggttg cctctggtcc tacgaggagc
     6061 tcggctgggg gggatactgg gcctgggatc cggtcgaaaa tgcatcgttc ctgccctggt
     6121 tggttggcac cgcgtacctg cattccgttc tgattcaaga gcggcgacac atgatgaagg
     6181 tttggaacgt cttcctcttg gcgttgacgt tcttcatgac gatattcggc acgtttctca
     6241 cgcgctcggg cctaatcgcc agcgtgcatt ccttcgcgcg ctccgacatc gggcagtatt
     6301 tcgtctggta catgggcttt ctgctcatcg gtatcgctgc tttgatgatt tggcggattc
     6361 ccaagctcaa ggccgacaac gagatcgagt cgctcgtcag tcgcgagttt gccttcttgc
     6421 tgaacaactg ggtgttgctc ggcatgatgg tgttcgtgct gatcgccacg accttcccat
     6481 tgctcagcca gtggattcga ggcgaagagg tcaccgtggg tcccggcttt tacaacaagt
     6541 ggatggttcc cctgggcatc gtgctcctgt tcctcgcggg cctcgggccg ctcgtcgcct
     6601 ggcgtaaggc gaccgggtcc aagttgcttc gcgcaatggc ggtcccggtg ggagttggtg
     6661 tggccgtcgc cgtgctgcag ttccttttcg gagcacgttt cggataccct ccgatcgtcg
     6721 aacccaccga gatttacgat accgctacgg gaactgctct ggcttggatg tcggccatag
     6781 ctccagtcgt ttccttcgcg acctgcgcgt tcgtactcgc aagcgttggc caggagttct
     6841 ggcgtggcgt tcgcgtccgc atgaaaaagg gcgaaaacat aggcaccgcg ctgactcgcc
     6901 tcgtatcgaa ggggcgccgc cgctatggcg gttacatcgt tcatgtcggg gtcgtcttca
     6961 tgtacatcgg cttcaccgga gcggcctacg atgtcgagca ggaaagcgcg cttcgccctg
     7021 gcgatacgat gtcgatcggt cagcacaccg tacggtacga ccaacctcgt atggaggcgg
     7081 acccgaacaa gcgaatggtc ttcacggaca tgacgctcat cgacgagagc ggtaacgaag
     7141 acggcgcggt cgccccagct aagttcatct atcgaaccca tccccagatg ccgactaccg
     7201 aggttgcgat tcgcagccgt cttgcagaag acgtctacgt gatcatgagc acggttgatc
     7261 catcgacccg gcgcggcacg ttccgcgtca tcatccgtcc tttggtggct tggatttggc
     7321 ttgggggaat cgtgcttctg ctcggagcga ttctcggcat gtggcccacc ggcaaggagc
     7381 ttctccgcga agaagtcgca cggccgcggc gttttcgctt ctcgcctgca accgccgtcg
     7441 tcgggctcgc gatcatcatc gccacggtga tggcctgggc cggtgttgca gcggcacaga
     7501 acaacagcag cagcagcctt cacgcaggct cggtcgtcat caacgatccg gtcgagcgac
     7561 agctcttcgg ccgcttgctc tgccagtgcg gagattgcgc ccggcttccg ctcgatactt
     7621 gcgcgtgtgg ttgggcggac gagaagcgga tcgaagtgcg cgagcagctc gccgctggag
     7681 tgccgcccac gaaaattcag gagtcctatc gagcgcagta cggggccaag gccttggcga
     7741 ttccctcgga cgaagggctg gaccgggcgc tctgggcagt ccctctaggc gcgatggtcc
     7801 ttgccgtcgg tttcatcgtc gtgcttggcc gccgttggca gcgacgcggt gtcgtcttgg
     7861 cgacctcagg cgcatccacc gccggggcgg agttcgaata tgatgcgagg ttagacgaag
     7921 agctcgatcg actggaggac agctgatgag cgagcttctc ccgtggattt tcgtcatcac
     7981 tgcgtcggtg gccttggtgg gcggcctcgt cagcttgtgg ctcagcttga gcatggcgct
     8041 ttccgacgaa gccctcggag gcgcacgcgc tcagctgatc accgatgcgc gtcgcgcgtt
     8101 gctggccgag aaagaagcgc tgcttcaaga gatccgtgac gtgtctttcg agcacgacgc
     8161 agacaagctc tcggattacg acttcaacga gatcaacgaa aagctgcgcg cgcaagcccg
     8221 gcacgttctt cacgagctgg acgcgggcgt cgaactgtac agggacgagg ccgaggcgct
     8281 gatcgccgcc cggcttgcgg acgaggtcta atgcgcatcg cagttctcat cggcctgctt
     8341 ctgtgggttc cttcgattgc atcggcacag ccagatcagg ccgtattcga ccgggtcacg
     8401 ggcaacgtac cagatatcgt gacctcacgg gtcgacccga gtcttccaac cggaacgatc
     8461 gtcgtcgagg tggtcgaccg agatggcggc ccggcgccga aacaagcggt tcagctcggt
     8521 ttgatggagc agtcgggagg gcgtgacagc aaggcatgcg tcaccgacga agcgggtacc
     8581 tgctcgttcg cggagctctc gaccgatgcg aaggattcgt accgcgtcaa cgtaccgtac
     8641 cagggtgccc gctacagctc gactccgttt cggctcgaca cgagcgcggg tcagcgcgtt
     8701 cgcgtgacgc ggttgccgac gacgacggac gacgagcgaa tcttccagct tcttggccgc
     8761 acgatcatcg agtttcgcga cgaccgtgcc cacattcagc aggaagcgag gctgttgaac
     8821 ctcggagact ccacttatgt gtttcccgaa gggggaatgc ggattcgcct gccggatggc
     8881 ttcaaggcat tcgattcgat ggccgtcatg accgaccagc tcctttccgc gactgacgaa
     8941 ggcctcgaaa tcagtggttc gattccaccc gggcgagtcg agctcatgtg ggcctatgac
     9001 gttcccgccg gcggcagctc tttatcgatc gagcaggcgg tgccttttgc caccatggag
     9061 tatcaggtca tcagcgacta cgtggacggg ctcagcatgg tcgtcgaggg ttttcaagtt
     9121 gcgcgggtcc acgagggcgg cgaccggcgc ttcctggttt cgggcttgat gcgacgccct
     9181 ggagatccgc cgatcgaccc actgcgcatt cacatcagcg ggattcctgg cggcggccct
     9241 ctcccatatc ttgcgagcgc gatcgcggcg attttcgtgt ttctgggaat ctggcttctt
     9301 tttcgtccat tggatcagag cgagattctg gccaaggtcc gcgaagagcg gcgtttggag
     9361 ctgctcgacg agatcgtttt gttggaaagc cagcggaaaa ccgaatcgat tgggccgggt
     9421 ttttacgaga accgtcggcg cgagctgacc gatgagctgg caatcgtgct tcgcatgcag
     9481 agcgagggaa cgggccattg atccgcggcg caaggccgcg tatgttcgcc gccgatcgcg
     9541 actagaagaa taggggagcc atggcagaag gcctgaatcg tgtgattctc atcgggaacc
     9601 tgggcgccga cccggagctc aagttcaccc agaacggaca aggcgttcta cgcctccgct
     9661 tggcgacgac cgagtcgtac gtcaatcgtg caggcgagcg acaacagcgc acggactggc
     9721 acacggtgac agtctggggc aagcggggcg aagccctgaa caacattctt tccaaagggc
     9781 gcagcatctg cgtcgaaggc cgcattcagt accgccagtg ggaagacaag gacggcaaca
     9841 agcgcaactc gaccgagatc gtcgcaacga acatcgttct ggtcggcagt ggccgtggac
     9901 gcgactccgg ggcgcccggt gacttcggtg gtggtgacac cggcggtggc ggaagcagcc
     9961 cacccaccga cgacttcggc gacgacgaca ttcctttttg aggcggggag tcggactcga
    10021 agttggctcc cttcgcggag cgcacgattc gttgagcccg accggtttac ggttgttgta
    10081 ctggcgatgc ggccggcgct tccaccggca gggcgtgctc cgcagctagg gcggcttcga
    10141 gcgcggcctt ctccgctcgc ttgagctcct tcgcttcacg agcgatgcga gccttctcgg
    10201 agacatacgc tgcggatgct gagacgacga tggccacttc gagggctaac gccagcacta
    10261 tgtacgggtc cggcttcgct ccctggacca tggcgagcac ccgaccgctt gcaataccag
    10321 tcgcgcagac caggaaggcg agcgatgcgg acagcctcag gcgctggaag agggcgccga
    10381 ggagcgcaaa cacgccgagc gctccccaaa gccccacgtt cagcgcgatc gactcgatgt
    10441 agagttggcc gtgctgcaca ccggagagac ccatccactc cggcaagatc gagccggcga
    10501 agagcgagag gagcgcccaa ccaatggagc cgacgccgag aaagaagagc acgttgcggg
    10561 ccatgggcca acgataccgc cgttcgcttt tcaaagaaag ttagtccagt cttcgtgcgt
    10621 gccgaaggtt cgcgccgacg tactattgac ccaaagaggc ctcgttccat cgagggctag
    10681 aggtcaggcc ctgatactcg cgggcaaagt gttcagcggg gaccggcgcg tcgagaaaag
    10741 cggcgaagcc ctggacgagg atgcgccaat cgaggtgcgc gagccggacc atccctatgt
    10801 gtcacggggt ggtgtcaagc tcgacggtgc actcgatctg ttcgcgtacg atcccgcgaa
    10861 caagacggcc gcggactttg gcgcatcgac cgggggtttc accgactgct tgcttcgacg
    10921 gggcgcatcg aaggtgtttg ccatcgatgt tggttacggt cagctgcaca acaagctgag
    10981 gcaggacgag cgggtggtcg ccatggaacg ctgcaacgcg cgacacttga aagccggtga
    11041 cctgcccgag cctatcgagc tcatcgtcat cgacgcctca ttcatcgggc tcgagaagct
    11101 tcttcccgcc gctcatgcgc tcctgccggt cggcggagac gtgctcgctc tcgtcaaacc
    11161 acaatttcag gttggacgcg acaaggtcgg caagggcggc gtagttcgag acgctgcact
    11221 tcgccgtgaa gccatcgcta cgctggtgac cgaagcagaa cgcctcggtt tcgagctcgt
    11281 agccgaagcg gacagtgtga tcacggggcc aaaaggcaat caggaaacgt tcatctggct
    11341 gcgtcgcttg gcggcggcac ccgctccacg attctgagat cgactggcat ccatccgcgg
    11401 tcagctttgc tctttccaac ctttgcccac caaacctgct cgcacgcgtt cggatgctgc
    11461 gcactgagcc cctcgaatgc atcgtcgaga gtgcacggcg tccaagcagt acccgcctcg
    11521 gacccaatca tgcaaaggcc cttcgagttg tagaacaaga aagatccaac ttcacctttg
    11581 gggaccgcca gaaaccttcg ctccacctga atgccttggt cccagacctt acgcgtcacg
    11641 aaaaggtctc gttttgcgac gagccgtcgg ggcttcaaga tcaagacatc actgtcctcc
    11701 acctcgatca gatcacctgc tttccaactc actgttgccg taatgaagga ggcatgattg
    11761 ggcttaagct tcagagtcac ccttgcgtat tgattgcccc atcgaagatt ccgatactcg
    11821 gccggatagg gccagctgtg cttggcgacc acgctggctt cgatttcgtg atcgcagccc
    11881 aagcccaacg atgg
//
LOCUS       DASRZF010000074         3708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3838128, whole genome shotgun sequence.
ACCESSION   DASRZF010000074 DASRZF010000000
VERSION     DASRZF010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3838128"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..851)
                     /gene="trmD"
                     /locus_tag="VFG22_03250"
     CDS             complement(120..851)
                     /gene="trmD"
                     /locus_tag="VFG22_03250"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HET6415288.1"
                     /translation="MRFEIVTLFPELFDALNTGLLGKARDAGTIDVQAITPREFTADR
                     HRTVDDSPYGGGSGMVLMPGPIADTLDHLDEARAGKPTHRVLLTPQGGLFSQAHARRF
                     STLDCLTLVCGRYEGFDERIRGLVDEEVSLGDFVLLGGEAAALAIIEASARLIPGVLG
                     NEASAQDESHSDGLLEYPQYTRPEEFRGQTVPDVLRSGNHAAIARWRRKESLRRTLER
                     RPELIESAELTEQDHKLLDEIRRGI"
     gene            complement(851..1393)
                     /gene="rimM"
                     /locus_tag="VFG22_03255"
     CDS             complement(851..1393)
                     /gene="rimM"
                     /locus_tag="VFG22_03255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HET6415289.1"
                     /translation="MNENREAAVSWIELGVVARPHGVNGEIRVHVFNPSSTLLEELGE
                     VFLIAEGDEEPSLVEVKSTRRGPKTLLMRLAGVNSYEDVEALRGCILCVPRAALPDLE
                     EGEYYHADLIGLQAFEGTELLGEVVDVLDYPSAECLKIERPDGFLEVPMLPRWLERVD
                     VDAGEVHLKDLDDIPLQKER"
     gene            complement(1508..1765)
                     /locus_tag="VFG22_03260"
     CDS             complement(1508..1765)
                     /locus_tag="VFG22_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HET6415290.1"
                     /translation="MSTDVAGDKLQDLIAYIASSLVDDPDAVEVNVIEEDRALVLELS
                     VAPDDLGKVIGKEGRTARAMRTLLAATSARLRKRAVLDILE"
     gene            complement(1766..2029)
                     /gene="rpsP"
                     /locus_tag="VFG22_03265"
     CDS             complement(1766..2029)
                     /gene="rpsP"
                     /locus_tag="VFG22_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HET6415291.1"
                     /translation="MVKVRLSRAGAKKRPYYHIVVTDKEKPRDGRFIEQIGTYDPSRP
                     ISEASVDYPRLDYWVGVGAQVSDRVRDVVNEHKKATTSEASAS"
     gene            2160..3680
                     /locus_tag="VFG22_03270"
     CDS             2160..3680
                     /locus_tag="VFG22_03270"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HET6415292.1"
                     /translation="MRCLLAVLALSLVLGCSRRQQAKSPLVEAPSLRLALVTDLKGYL
                     EPCGCTSDPLGGIDRLAAQIRALREGTSPVLVLIAGDLLFDTAQLEPSRVDQATRNAK
                     TLVQILNRLKVAAVLPGASDRAQSAETLTALRELSDFPWLAMDKAVDVLQTDAGALRV
                     AVVGARPGSERAAVADAVTALRPETDLSIVLVDGSRREANRTGAIDGVDFVIQGGLDE
                     DEPMPPRRAGKAWVLHASRQGQGLTVVDITIKTPDAGFADRSEWSRSVQAKQLDQQIE
                     DLATKITNWEKSGEVQAADLTSQRARLDALREARASIDVPELGAGENTLSATWIPLSK
                     SAPKDPQVEKLMREHDKVVNEANRLAFADLQPPPLGPDDIAYVGSPACSACHQEAYAW
                     WRGHGHGNAYLTLQEGNKEFNLDCVGCHVTGYQEPGGSTVTHNMNGALVNVGCESCHG
                     PGAAHSKDPEKFSILRDTPESTCVRCHNSQHSDLFDYEGYKRTLVVPGHGLPTAVR"
BASE COUNT          674 a         1167 c         1196 g          671 t
ORIGIN      
        1 cacagcgatt gggcgggagg ctagacgtag ggagtcgtag agagacggcc gagcgggtcc
       61 caatcgcgag cacgtgcgtc gcgagccgcc ctccgatggt cacagtgcca gcgccggggt
      121 cagatccctc tgcggatctc gtcgaggagc ttgtggtcct gttccgtcag ttccgcggac
      181 tcgatgagct cgggcctgcg ctccaatgtt cgccgcagcg actccttgcg tcgccagcgc
      241 gcgatggccg cgtggtttcc tgagcgcaga acgtcaggca ccgtttggcc tcgaaactcc
      301 tcgggtcgcg tgtactgcgg gtactcgagc aagccatctg aatgggattc atcttgggcg
      361 gacgcctcgt tgccgagaac tccgggaatg aggcgcgcgc tggcttcgat gatggcgagc
      421 gcagcggcct cgccgccgag aagcacgaag tctccgagtg agacctcttc gtcgacgagc
      481 ccgcgaattc gctcatcgaa gccttcgtac cgaccgcaaa ccaaggtgag acaatcgagt
      541 gtcgagaacc ggcgtgcgtg ggcctgcgag aagagcccgc cttgcggggt gagcagtacg
      601 cggtgcgttg gcttgcccgc gcgggcctcg tcgagatgat cgagtgtatc cgcaatcggt
      661 ccgggcatca gcaccattcc gctgccaccg ccgtacgggg agtcgtccac cgttcgatgc
      721 cggtccgccg tgaactcgcg cggcgtaatc gcttgcacgt cgatcgtccc cgcatcccgc
      781 gccttgccga gcaggccagt gttcagcgca tcgaaaagct caggaaagag cgtgacgatt
      841 tcaaaacgca tcagcgctcc ttctgaaggg ggatgtcatc caggtctttc agatgcactt
      901 cgcccgcatc gacatcgacg cgttcgagcc aacgcggaag catcggcact tcgagaaagc
      961 cgtcgggtcg ctcgatcttg aggcattcgg cgctcggata atcgaggaca tcgacgactt
     1021 caccgagcaa ctccgtgcct tcgaatgctt gaagcccgat caggtcagcg tgatagtact
     1081 cgccctcttc gaggtccggc aatgccgcgc gaggaacgca gagtatgcaa ccgcgaagcg
     1141 cttcgacgtc ttcatacgag ttgacgccgg ccaatcgcat gagcaaggtc ttggggccgc
     1201 gccgcgtgga cttcacctcc accaacgaag gctcctcgtc tccctcggcg atcagaaaaa
     1261 cctcgcccag ctcttcgagt agagtcgagc tcgggttgaa gacgtgaacg cggatttccc
     1321 cgttcacgcc gtgaggccgg gcgacgacgc ccagctcgat ccaggaaacg gctgcttcgc
     1381 ggttctcatt cacggagact ttttatacca aattggggag gtccgggcgc gctcctggat
     1441 caggaagccc ggtctaggga ccagcagatc gattcaaagt ggaccagcgg ctcgcggcgc
     1501 cagcgggtca ctcgaggatg tcgaggacag cccgcttgcg aagtcgcgcc gaagtggccg
     1561 ccaggagcgt gcgcatcgcg cgagccgtcc ggccttcctt gccgatgacc ttgccgaggt
     1621 cgtcgggcgc cacgctgagc tcgagcacga gggcccgatc ttcttcgatc acgttgactt
     1681 cgaccgcgtc ggggtcgtct acgagcgagc ttgcgatgta cgcgatcagg tcctgaagct
     1741 tgtctccggc cacgtcagtg ctcacttaag aagccgaggc ttcgctcgtc gtcgcctttt
     1801 tgtgctcgtt gaccacgtcg cggacgcgat cgctgacctg ggcccccacg ccgacccagt
     1861 agtcgagacg ggggtagtcg acgctggcct cgctgatggg gcggctggga tcgtaggtgc
     1921 cgatctgctc gataaagcgc ccgtcgcggg gcttttcctt atcggttacg acgatgtggt
     1981 agtaggggcg cttctttgcg ccagcgcgtg acagtcgaac cttgaccatg gattcctcgt
     2041 aagcggcggg ccgggcccac ccgaaagacg ccggaagcta gtcagagcct gcctccccgt
     2101 caagccgccg ccccgacagg gtttttgcgg cggggcgttc cgttgctaga accagaggga
     2161 tgcgatgctt gcttgccgta ttggccttga gtctagtgct gggctgtagc cggcgccaac
     2221 aggcgaagag cccgctggtc gaggccccga gcctgcgcct cgcgctggtg accgacctca
     2281 aagggtattt ggagccctgt ggttgcacca gcgatccgct cggaggcatc gatcgactcg
     2341 ccgctcaaat ccgagcgctt cgcgaaggca cgtcacccgt cctggttctg atcgccgggg
     2401 acctgttgtt cgacacggct cagctcgaac cctcgcgcgt cgaccaggcc acgcgcaatg
     2461 ccaagacgct cgttcagatc ctcaatcgct tgaaggtcgc cgcggtgctt cccggagcga
     2521 gcgatcgagc gcagtccgcg gagacattga ccgcccttcg agagctctct gacttcccct
     2581 ggctggccat ggacaaggcg gtcgatgttc tgcagaccga tgccggtgcg ctgcgtgtcg
     2641 ccgtcgtcgg cgcgcggccc ggatccgaac gcgcggccgt cgcggatgcg gtcacagcct
     2701 tgcggccgga gactgacctc agcatcgtgc tcgtagacgg gtcgcgacgc gaggcgaacc
     2761 ggaccggcgc gatcgacggc gtcgacttcg tgattcaggg aggactcgac gaggacgagc
     2821 cgatgccgcc gcggcgtgcg ggcaaagcct gggtgcttca cgcgagccga caaggccaag
     2881 gtctcaccgt ggtcgacatc accatcaaaa cccccgacgc aggttttgcg gaccgcagcg
     2941 aatggtcgcg ctccgtgcaa gcgaagcaac tcgatcagca aattgaggac cttgcgacga
     3001 agatcacgaa ctgggagaaa tcgggcgagg tgcaagccgc cgatttgacc tcacagcgcg
     3061 ctcgactgga cgcgcttcgc gaggcgcggg cctcaatcga tgtgcccgag ctcggtgcgg
     3121 gcgaaaacac gctgtccgcg acttggattc cgctttccaa gagtgcgcca aaggatccgc
     3181 aggtcgaaaa gctgatgcgc gaacacgaca aagtcgtgaa cgaagcgaat cgtctcgcgt
     3241 tcgccgacct tcagcctccg cctcttgggc ccgacgacat cgcttacgtg ggctcgccgg
     3301 cttgcagtgc ttgccaccag gaggcctacg cgtggtggcg ggggcatggt catgggaacg
     3361 cgtatctcac gcttcaggaa ggcaacaagg agttcaattt ggactgcgtc ggttgccacg
     3421 tgaccggata tcaggagccg ggcggctcga ccgtgaccca caacatgaat ggggctctcg
     3481 tgaacgtcgg ctgcgaaagc tgtcacggcc ctggcgcggc gcactcgaag gatcctgaga
     3541 aattcagcat cctccgggac acgcccgagt cgacctgcgt gcggtgccac aactctcagc
     3601 acagtgacct cttcgactac gaaggctaca aacggacctt ggtggttccc ggtcacggct
     3661 tgccaacggc ggttcgctga gagggagccg ccaatctcac gcgtcgag
//
LOCUS       DASRZF010000075         4139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2807247, whole genome shotgun sequence.
ACCESSION   DASRZF010000075 DASRZF010000000
VERSION     DASRZF010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2807247"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VFG22_03275"
     CDS             <1..418
                     /locus_tag="VFG22_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415293.1"
                     /translation="RTSLARVVFPAEPVALGARWEARKPLKLYGFEIQQTDRYTLRDR
                     SGNTVKLGVDIIHRAPEQNLSFAEEGMELALKSLSTTASGEVDLRLNALEGNARLKGQ
                     SQESLTVTKGDQTESIELDSAFELSMDVTYDASQAP"
     gene            528..1688
                     /locus_tag="VFG22_03280"
     CDS             528..1688
                     /locus_tag="VFG22_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415294.1"
                     /translation="MATEAQKTWDTGRASRAPEISIKNGRGQLATTARPRRLVQNLAH
                     RVDGLLVSQIAKLQPSDLKEPPELVHDLTAFASSADYQTYRACQSDLFPNPGRIDVKK
                     RWLAPHRVGFAEWSWQSLHPLIFAGIEHEYRSHRDNQNALLRTLNLRIKGKPMAILVN
                     GFSSGHHLMERVAWPIREFNRQGIGVSLFALPFHGPRGHAFPPEWPQQDTAFTIEGFR
                     QAIWDLQIAIRAMREAGASEVGVVGMSLGGYTASLLATVTSDVDFVLSYIPIASIPDV
                     MNDNDLVPGTGDVQRTLYEGYREQLVPITPICREPKVEPKRVSVISGEFDRLATMQHG
                     KRLADHFGTELVTFPGGHIVQNRRAQAFRRSLEGFRAAGVLPGKRCTRRAKR"
     gene            complement(1750..2289)
                     /locus_tag="VFG22_03285"
     CDS             complement(1750..2289)
                     /locus_tag="VFG22_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415295.1"
                     /translation="MKFLLCVCAIFALASCKDPAFIPGTGTLPDCNEPPVTDLNDTVW
                     FNTGPATVLTAGCLDVEPDTEFESCPENWAFKQDGNEIDIVVDEYRVNGRFCGDKLYL
                     EGGWWLSVRNERGRCDYGDEDGDEFGMQAGGNVLTYTASNPENFGFAEMTGILLLEGN
                     CSVSYDATFQQVWSPPPPS"
     gene            2369..2632
                     /locus_tag="VFG22_03290"
     CDS             2369..2632
                     /locus_tag="VFG22_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415296.1"
                     /translation="MDTRLRDDLRTPIRDTSSRLIRDLARSTGARFARPLLGPPPQLV
                     HAQQREHAVAKRVDDERVSEAFSTARIGFIWPVVSATQSSEAT"
     gene            2999..3826
                     /locus_tag="VFG22_03295"
     CDS             2999..3826
                     /locus_tag="VFG22_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415297.1"
                     /translation="MDALTREQELEGGRDPNGLSFFDSHPTTPDRARDAREYAAKLEV
                     ASADRIAIDRTAFVQRLDGLALGESVRAGLFLDGRFLHPELGFGISFPDDWEHENSPS
                     AVVAQPEDGSAVLAVQLAGKGDDPTAFADQFETKVPFLERSAVEKINQLPAVIGIAQV
                     VDDGEEINLFLTWIAKDGLVYRVLGAAPTSRWNEHHPNFEAAARSFHALSDRELRELH
                     VNRLRLVKAHAGETLTAIAARSSSQWSAEKMAVANDLSTSAPLPDGETIKISKREPY"
BASE COUNT          823 a         1337 c         1237 g          742 t
ORIGIN      
        1 gcgaacgtcg ctggcacgtg tcgtttttcc tgccgagccg gtggcactgg gcgctcgttg
       61 ggaggcccgc aaaccgctga aactctacgg cttcgaaatt cagcagaccg accggtacac
      121 gcttcgggac aggagcggca acacggtgaa gctgggtgtc gacatcattc acagggcgcc
      181 cgaacagaac ctgtccttcg cagaagaggg tatggagctc gctttgaaat cgctttcgac
      241 gacggccagc ggggaggtcg acctcaggct caacgcactc gagggtaacg cgcgcctaaa
      301 gggccagtcg caggagagcc ttaccgtgac gaagggcgac cagacggaga gcatcgagct
      361 cgacagcgcg ttcgagctca gcatggacgt gacctacgac gcctcgcaag caccctgacc
      421 ggtaagcgtc cacttgcgtt cggcgcaatt tttgcgcccc aaaaacctca tgcaccggcg
      481 acaacaattg cactgaacgc atagtgtcga tataccacta ggccatcgtg gcaaccgaag
      541 ctcagaagac atgggacacc ggccgcgcaa gcagagctcc tgagatctcg atcaagaacg
      601 gccgcggcca gctcgcgacg acggcgcgac cacggcgctt ggttcagaac ctcgcgcata
      661 gggtcgacgg ccttctagta tctcagatcg ccaagctcca gccgtccgac ctcaaggagc
      721 cacccgagct cgttcacgac ctaaccgcgt ttgcatcgag cgcggactac cagacctatc
      781 gagcttgcca gagcgatctc ttccccaacc cgggacggat cgacgtcaag aagcgatggc
      841 tggcgccgca ccgtgttggc ttcgccgaat ggagctggca gagcctccac ccgctgattt
      901 tcgccggcat cgagcacgag tatcggagcc accgggacaa ccagaacgcg ctgcttcgca
      961 cgctgaacct tcgaatcaag ggcaagccga tggcgatcct cgtcaacggg ttttcgtcgg
     1021 gtcaccattt gatggagcgg gttgcctggc cgattcgcga gttcaatcgc caggggattg
     1081 gcgtctcgtt gtttgccctt ccgttccacg gcccgcgcgg ccatgcgttt ccacccgagt
     1141 ggccgcagca ggacaccgca ttcaccatcg aaggtttccg ccaagcgatc tgggacctgc
     1201 agatcgcaat ccgcgcgatg cgcgaagcag gcgcaagcga agtgggcgtc gtcgggatga
     1261 gtcttggcgg ctacacggcg tcgctgctgg cgacggtgac ctctgacgtc gacttcgtgc
     1321 tttcgtacat tccgatcgca agcatccccg acgtcatgaa cgacaacgat ctcgttcctg
     1381 gcacaggtga tgttcaaagg acgctctacg aaggctaccg cgagcagctg gtaccgatca
     1441 cgcccatctg cagagagccg aaggtcgaac caaagcgcgt ttcggtcatt tcgggagagt
     1501 tcgatcgtct agcaacgatg cagcacggga agcgtctcgc cgatcacttt ggcaccgagc
     1561 tggtgacgtt ccccggcggg cacatcgtcc agaaccggcg agcacaagcg tttcgacgct
     1621 cgttggaagg cttccgagcc gccggcgtcc tgccgggaaa gcgatgcacc aggcgcgcga
     1681 agcggtagag ctcgagcacg ctacgcagcg cactctcgat gggttctgcg gcaagcgacg
     1741 ctcgtccgct caactcggtg gtggggggga ccaaacctgc tggaaggttg catcgtaact
     1801 aacgctgcag ttgccctcca agagcaggat gcccgtcatc tccgcgaaac cgaagttctc
     1861 aggattggac gcggtgtagg tcaagacgtt gcccccggct tgcatgccga actcgtcccc
     1921 gtcctcgtcc ccgtagtcgc agcggccccg ttcattcctg accgaaagcc accagccgcc
     1981 ctcgagatag agcttgtcgc cacagaaccg gccgttgacg cgatactcat cgacaacgat
     2041 gtcgatctcg tttccgtctt gcttgaaggc ccagttttcg ggacacgatt cgaattcggt
     2101 gtcgggttct acatccaggc atcctgcggt gagaaccgtt gcagggcccg tgttgaacca
     2161 gaccgtgtcg ttgagatcgg tcacaggcgg ctcgttgcaa tcgggtagcg tacccgttcc
     2221 agggatgaac gcgggatcct tacagctcgc aagagcgaag atcgcgcata cgcagagcaa
     2281 gaatttcatg gccgttgcgt accacatttg catggcttga ccagccggat ccctaatccg
     2341 acgtgcgctt tcttgcaggg gcggtcctat ggacacccga cttcgggacg atctccgaac
     2401 gccgattcgt gatacgagct cgcgcctcat acgagacctc gcaaggtcga ccggcgcgcg
     2461 cttcgctcgc ccgctcctcg ggccacctcc acaactggtg cacgcccaac agcgagaaca
     2521 cgcggtagcc aaacgcgtcg acgacgaacg tgtctccgag gcgttctcga cagcgcgcat
     2581 cggattcatt tggccggtcg tcagcgcaac ccagtcgtcc gaagcgacgt gaatcgtcgc
     2641 gtcttgttcc gggccattct cgcgagcaac gaagcccaac gtcgtgcctt ggcgaaaaac
     2701 tagcgtccaa gtgccgctat cctccccgct gagctcgaat cggaacacgg cattggggag
     2761 tgtcgcagcc tccggcttgg ccagcgcagc cgcctcgaac gctgttccgc agcttgaaaa
     2821 gccgcgctca atgaccacac tcgggtcatg aaagaacccc ttgctgcgca gagagagccg
     2881 ccggcccgca aggtgccgag gcggaccgtt gccctcacct gcgcggcgct gaccctcggt
     2941 ttgctagcct gcgccgcaaa cccgggcacc gcgcgcaccc gccgccttgg cgagcagcat
     3001 ggacgcgctc acccgtgagc aggagctcga aggtggacgc gatccaaacg ggctgagctt
     3061 cttcgattcc caccccacca caccagaccg cgcacgcgat gcccgtgagt atgccgccaa
     3121 gctcgaggtg gcgtcggccg atcgcatcgc catcgatcgt accgccttcg tacaacgcct
     3181 cgatggcctc gccctcggcg agtcggttcg ggcagggctg tttctagacg ggcgtttcct
     3241 ccatcccgag ctgggtttcg gcatcagctt ccccgacgac tgggagcacg agaacagccc
     3301 cagtgccgtc gtcgcgcaac ccgaggacgg gtccgccgtt ctcgcggtgc agctggccgg
     3361 caagggggac gatcctacgg cattcgctga tcagttcgaa acaaaggtcc cgttccttga
     3421 acgaagcgcg gtcgagaaga tcaatcaact gccggcggtc atcggcattg cccaagtggt
     3481 cgacgacggc gaagagatca atctcttcct cacctggatt gccaaggacg gactcgtgta
     3541 ccgagtcttg ggggcggctc ccacctcgcg ctggaacgag catcatccga acttcgaggc
     3601 cgccgctcgg agcttccatg cgctctcgga ccgagagctc cgtgagctgc acgtgaatcg
     3661 cctgcgcctc gtgaaagccc acgcaggcga aacgctgact gctatcgccg ctagaagctc
     3721 gagccaatgg tcggcggaga aaatggccgt tgcaaacgat ctgagcacga gcgcgccgct
     3781 gccggacggt gagacgatca agatcagcaa gcgagagccg tactgagctc ggagacatgg
     3841 cccgccgacc accaaggcct gcgcgttact gcgccgaaat ctcggtctcg gagatcttct
     3901 cggcttcgac gtccgccggg tagtacagca tgtcgttccg acctttgtcc gaatagagcc
     3961 gcgcctcaag cgcgtcacaa cccctcgatt gcggcggtca gacagctctg gtaaggcccg
     4021 taccgtaagc tcttttcagt tgcgggtgac cgcagctcgc cacggatcag tcatgctcca
     4081 cttcgcctac gacggatcga ttcacggcga ctgggttgct cgctatgcga tgcaaatgg
//
LOCUS       DASRZF010000076         3790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4184342, whole genome shotgun sequence.
ACCESSION   DASRZF010000076 DASRZF010000000
VERSION     DASRZF010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3790
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4184342"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VFG22_03300"
     CDS             complement(<1..379)
                     /locus_tag="VFG22_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415298.1"
                     /translation="MKNSKWIVGVSSLILAIVGCGGDDQGSGADGNLSILLKAEETIV
                     NGLQAGTGEENIRDGFDVDFSRYIVAIGLVQMNQVGGSNPQASSVIAVADFTSLPTTL
                     PELTRFDGIPTGQYTDFGFATPMP"
     gene            complement(405..1082)
                     /locus_tag="VFG22_03305"
     CDS             complement(405..1082)
                     /locus_tag="VFG22_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415299.1"
                     /translation="MTLSQATLGFGAIYFCATDSALSSRCEVAILEYTEGLTLNGLDP
                     TEQSIGSLFGTTGTVRTAFFDYGVVWLLTEPLPEALAGVPGGPAVVRFESPSYVPEGH
                     SGRFSGSATRIEDASICCPDALTCPSSYTFEANVDVLVVNPGTPAVNGARTTQEITTA
                     PLALTITFDPNTWWQAVDFRRLATLDDGSGPVVLSPEDPDYSALVISMTANALPRFVW
                     SSPAMEP"
     gene            complement(1214..>3790)
                     /locus_tag="VFG22_03310"
     CDS             complement(1214..>3790)
                     /locus_tag="VFG22_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET6415300.1"
                     /translation="ILWVNAKVRAQEGATEAFTDTDMAAEAELHPPHLLVAPPPVYPP
                     GREDEGLHPTVILRITVTAEGGVTDVVVEHSAGSDFDAEAARAVEGWTFEPATRGDTA
                     ISSRVRVAVHFEPVSAGVPHQGEMHEPQAAPHVEQTPPPENVPAEVAPAKVVDDDKPL
                     KNQQAGFSTVAEIDREKLRAEDRGAGDFTIDRKILDAAPAMTANDLLRRAPGLYVANV
                     EGDAAGGFFYLRGFNAEHGQDIELKLGDVPLNQPSNVHGQGYTYLDFIIPEVVRELRV
                     IEGVYDPAQGDFAVAGTVAFDLGVEQRGIYAKTQFGSFRTFRQVAIFAPKGKDPDTFG
                     AFQFQRSDGFGQNRDGLNGSAMFQVGFGREEWRFRLHGSFWGTRYNSAGVLRADDIDE
                     GTVGFYDTYPYATARAQNAYNAGAILGVHGESRETNGKNTSFDVWIQLLDFRIAQNFT
                     GFLERSRVNPGWVGRGDLIEQRDRRLALGGNARHRMARFEPGKNASGSLEMGVSARVD
                     LIDPQQQNLVEAPANQTWDQRIDANIVQSDIGLWVDLDWDFSKYFNVKAGARTDVLFF
                     DVDDKLGNFVPDFRPDGFIPGFRRTAAGVFAGPRLALAVKPTDWIDIIGAYGKGFRSP
                     QAITLVDGEIAPFSEVRSLDFGVRFRLGPNEELKLTLTGYRTWLNQDVAFDAAEGRLE
                     NIGPTSRTGFVFYAVTNPLPWLVGSVSVTYVDAILEGPPPPTVENPTPPFEEGDLLPF
                     VPPWVVRLDVGAGDELVDLWRYPLRGHLGLGYTFIGARPLPFSQTAAPFSLLDLSAKL
                     SWWFLEVGFQVFNVLDNQYAGNEFAFVSNWDPGSVPSRIPARHISAGRPRMFLFTIGI
                     QL"
BASE COUNT          712 a         1146 c         1222 g          710 t
ORIGIN      
        1 tcggcatggg ggtcgcgaaa ccgaaatcgg tgtactgacc cgtcgggatt ccgtcgaagc
       61 gggtgagctc gggtaacgtc gtgggaaggc tcgtgaaatc ggccactgcg atgacgctgc
      121 tagcttgcgg gttgcttccg ccgacttggt tcatctgcac gagcccgatg gcgacgatgt
      181 atcggctgaa gtcgacgtcg aatccgtcgc ggatgttctc ctctcccgtg cccgcctgaa
      241 gtccgttgac gatcgtttcc tcggctttga gcagtatcga taggtttcca tcggctccgc
      301 tgccttggtc gtcgccaccg cagccgacta tcgcgaggat gagcgacgac actccaacaa
      361 tccatttcga attcttcata ggactctcct tctttggcga tcggtcatgg ttccatggct
      421 ggcgacgacc aaacgaatct ggggagcgca ttggccgtca tcgagataac cagcgcgctg
      481 taatcgggat cttcagggct gagaaccacc ggccccgagc cgtcatcgag cgtagcgaga
      541 cggcgaaagt cgactgcctg ccaccaagtg tttgggtcga aggtgatggt gagcgcgagc
      601 ggcgcggtcg tgatctcctg cgtggtccgc gccccgttga ctgcgggggt gccggggttc
      661 acgacgagaa cgtcgacgtt ggcctcgaac gtgtaggagc tggggcacgt cagcgcgtcc
      721 gggcaacaaa tcgatgcgtc ctcgatgcgg gtcgcgctac cggagaaccg gcccgagtga
      781 ccctcgggaa cgtaactcgg gctctcgaaa cgcacgacgg caggtccccc gggcacccca
      841 gcgagggcct cggggagcgg ttctgtgagc agccagacga ctccgtagtc gaagaacgcg
      901 gtgcggaccg tgcccgtcgt tccgaagagc gaaccgatcg attgctcggt cgggtcgagt
      961 ccgttcaatg tgaggccttc ggtgtactcg aggatcgcga cctcgcaccg actggaaagc
     1021 gcgctgtcgg tcgcgcaaaa gtagatcgca ccgaacccga gcgtggcctg agagagggtc
     1081 acctcccaac cgtccttcac aaacgtctga ggctcggtcc cctaaactcg aaacgggatc
     1141 tcgacgaagg tgccgcccgt ctgaccgcag cccgcggcca gcgcagagat cgcggcgatc
     1201 cagaagagtc ggttcatagc tgaatcccaa tcgtaaaaag gaacatcctt ggcctgccgg
     1261 cgctgatgtg gcgcgcaggg attcgcgatg ggaccgatcc gggatcccag ttcgatacga
     1321 acgcgaactc gttcccggcg tattgattgt cgagcacgtt gaagacctgg aagccgactt
     1381 cgaggaacca ccaagagagt ttcgccgaca gatcgagtaa cgagaatgga gcggcggttt
     1441 gcgagaacgg gagcggcctc gcgccaatga aggtgtatcc gagcccgagg tgcccgcgga
     1501 gcggatacct ccagaggtcg accagttcgt ctccggcgcc cacgtcgagg cgaacgaccc
     1561 aaggaggaac gaacgggaga aggtcgccct cttcgaacgg tggagttggg ttctcaacgg
     1621 tcgggggcgg cggtccctcg aggatcgcat cgacgtaagt gacggagacg gagcccacga
     1681 gccatggcag cgggttcgtg accgcgtaga agacgaaccc cgtccggctc gtggggccga
     1741 tgttctctag acgcccttcg gcggcgtcga aggcgacatc ttggttcagc caggtgcggt
     1801 agccggtgag ggtcagcttg agctcctcgt tggggcccag ccggaatcgc accccgaagt
     1861 cgagggaacg cacctcgctg aaaggggcga tctcgccgtc gaccaaggtg atcgcctggg
     1921 gcgagcggaa acctttcccg taggcgccga tgatgtcgat ccagtcggtt ggcttcaccg
     1981 cgagcgctag tcgtggccct gcgaacaccc ctgcggccgt acggcggaag cccgggatga
     2041 agccgtcagg gcgaaagtcc ggaacgaagt tcccaagctt gtcgtcgacg tcgaagaaca
     2101 gcacgtcagt acgcgcgccc gccttcacgt tgaagtactt gctgaagtcc cagtcgagat
     2161 cgacccagag accgatgtct gattggacga tattcgcatc gatgcgctgg tcccatgttt
     2221 ggttcgccgg cgcctcgacc aggttctgct gttggggatc gatgaggtcg acacgcgcgg
     2281 atacacccat ctcgagactg ccacttgcgt tcttccccgg ctcgaagcgt gccattcgat
     2341 ggcgggcgtt gccgcccagc gcgaggcggc ggtcacgttg ctcgatgagg tcgcctcggc
     2401 ccacccatcc cgggttgacc cgcgaccgct cgagaaagcc cgtgaagttc tgcgcgatgc
     2461 gaaagtccag aagctggatc cagacgtcga aactagtgtt cttcccgttc gtctctcgac
     2521 tctcaccgtg cacgcccaat atggcgcctg cgttgtacgc gttctgggcg cgtgcggttg
     2581 catatggata ggtgtcgtag aagccgacgg tgccttcgtc gatgtcatcc gcgcgcagaa
     2641 cgcctgcaga attgtaccgc gttccccaga acgaaccgtg aagccgaaat cgccattcct
     2701 cgcgtccaaa tccgacctga aacatcgcgg agccgttgag cccatcgcgg ttctgtccga
     2761 atccgtcaga ccgttgaaac tggaacgcac cgaacgtatc cggatctttg cccttggggg
     2821 caaagatggc cacctgacga aaagttcgga aggagccaaa ctgagtcttg gcatagatgc
     2881 cgcgctgctc cacgccgaga tcgaacgcga cagtcccggc gacggcaaaa tccccttgcg
     2941 ccggatcgta cacaccttcg atcacgcgaa gctcacgaac gacctcgggt atgatgaagt
     3001 cgagataggt gtagccctgg ccgtgaacgt tggacggctg gttgagaggt acgtcaccga
     3061 gcttcagctc gatgtcctgc ccgtgctcgg cgttgaagcc gcggagatag aagaacccgc
     3121 cggcggcgtc gccctcgacg ttggcgacgt agaggcctgg cgcgcgacgt aagagatcgt
     3181 tcgccgtcat cgcgggtgcc gcatcgagaa tcttgcggtc gatcgtgaaa tcgccggcgc
     3241 cacgatcctc tgcgcgaagc ttctcgcggt cgatttcagc gactgtgctg aagccagcct
     3301 gctgattctt gagtggtttg tcgtcgtcga ctaccttcgc gggcgcgact tcagccggga
     3361 cgttttcagg cggcggtgtt tgttcgacat gcggggcggc ctgcggttcg tgcatttcac
     3421 cctggtgcgg taccccagcg gaaacaggtt cgaagtgtac cgcgacccga acacggctcg
     3481 aaatcgccgt atccccacgg gtcgcgggct cgaacgtcca gccctcaacc gcccgggcgg
     3541 cttctgcgtc gaagtccgag ccagccgagt gctcgaccac cacatcggtg accccgcctt
     3601 ccgctgttac ggtgatccga aggatgacgg tcggatgaag tccctcgtcc tcgcgaccgg
     3661 gcgggtagac gggaggcggt gccacgagga ggtggggtgg gtgcagctcg gcttcggcgg
     3721 ccatgtccgt atcggtgaac gcttctgtgg ctccttcttg cgcacgcacc tttgcattca
     3781 cccagaggat
//
LOCUS       DASRZF010000077         5495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3153177, whole genome shotgun sequence.
ACCESSION   DASRZF010000077 DASRZF010000000
VERSION     DASRZF010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 5495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5495
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3153177"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4151
                     /locus_tag="VFG22_03315"
     CDS             <1..4151
                     /locus_tag="VFG22_03315"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET6415301.1"
                     /translation="SPTGSGKTLAAFLCAIDDLVKRGLSEGLEDRVSVLYVSPLKALS
                     NDIEKNLQMPIEGIRATLEELGYGDLEIRTAVRTGDTPSHMRTKMVKKPPQIFVTTPE
                     SLYILLTSESGRRMLSDVRTVIVDEIHAVVTNKRGAHLALSLERLEALVGRSITRVGL
                     SATQNPIEDVARFLVGVREDEASMHCRIVNVGHQREMDLAVELPGSPLETVMSLEVWE
                     EIYARLAALIETHETTLIFVNSRRLAERATRYLSELLGEESVTSHHGSLSRKHRLDAE
                     RRLKSGELKAVVATASLELGIDVGDVDLVCQLGSPHSVATFLQRVGRSGHWHGGTPKG
                     RLFPLSRDDLVECAALLRCVRRRELDRIAIPEGPLDILAQQIVASTAATETWSEASLL
                     ALVRRAWPYRNLSEASFAQVVQVLSEGFATSRGRRGAFLHRDEVNRQIRARRSARLTA
                     ITNGGAIPDNFDYEVRLEPEGLRVGSVNEDFAIESMAGDIFQLGNVSYEILRVEPGVV
                     RVADAKGKPPSLPFWFGEAPSRADVLSISVNAIRDGIEARHQDLDSVAEWLERETGIG
                     ELGAKQVTDYLVASRIALGAMPTRETVVLERFFDETGAMHLVVHAPFGSRINRAWGLA
                     LRKRFCRSFNVELQAAATEDAIVLSLGPTHSFPLEDVFRFLRAENVRGVLIQAMLDAP
                     MFQTRWRWNATRALAIARFRGGKKVPPPFQRMQADDLLGLCFPDQVACLENVAGDREI
                     PDHPLVQQTIEDCLHEAMDIDGLESLLERLHAGELELLARDLTEPSPLAQEILNARPY
                     AFLDDAPLEERRTQAVMARRWLDPTRASDLGALDQAAIDQVRKEAWPEPRDAEEMHDS
                     LLLHNCLMETDAADAGWEAWLAELREAGRATRFITEGHVAWTAAERLPLLQGAWPSGR
                     AETGVAVPERHRRECWAKEDAVREIVRGRIQASGPTTGKRLAEILGLDVSLVDAALAA
                     LEGEGFVLRGRFTPASSAEEWCERGLLARIHRYTLNRLRKEIEAVSAADFLRFLVTWQ
                     HAAPEARMEGPEGLAAIIEQLEGIEAGAAAWESDILPVRMEGYDPSWLDQLCICGRVT
                     WSRRTPPLGRASSPIRTSPIAFTRREQTVTWRFRASGEQPASSRATQTLETLRRSGAS
                     FFADIVSETGLLTTEAEGALGELVSLGQVTADGFTGLRALLAPDPKRPRGGRRALAAY
                     SMEAAGRWSVLPEAPEEHDVESIAWALLRRWGVVFRRVLDREGDLPPWYALLRVYRRL
                     EAQGRIRGGRFVAGFAGEQYALPEAVTALRKSRRRSRTGELLSISAADPLNLVGILTP
                     GQRITATPKNRILIRDGVPIAFREGSETHFLEDPSDQRWALTQALQRQSVPRAVQAYL
                     GNRP"
     gene            4304..>5495
                     /locus_tag="VFG22_03320"
     CDS             4304..>5495
                     /locus_tag="VFG22_03320"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HET6415302.1"
                     /translation="MTDPLDKCFSEVTIKSLTLRNRLIKAATFEGKSPGGIPSEALVR
                     FHERIGQGGIGMTTIAYCAAEPDGRIHENMMYMHEGIRPELERFIRTVQATGAKVSGQ
                     LGHCGAFTKNLEFSGKRPLGPSKGTNALGLAYGLSRIAEMTKPQIRERVQVIGRAAAF
                     MKSVGFDAIEIHFGHGYGISQFISPKTNQRKDEYGGSLQNRMRFALEVLAEVRKSVGD
                     DYPLLGKISMTDGVPGGVTYEDSVEIAAMLEAGGLDVIICSGGTSSMNPMLLFRGDSM
                     VPGLIKHEKSRLMKIGIRLAAPLMFKDYPYEEMYFLEEAQRIRDRVQCGVCYIGGVCT
                     AASIRTVMGAGFDFIQLGRGLIFDPDLPKRAQSDANYENGCNHCNQCATLIEAPGGIY
                     CVERP"
BASE COUNT         1033 a         1722 c         1773 g          967 t
ORIGIN      
        1 catcgcccac tggatcaggg aaaacgctgg cggcgtttct ttgcgcgatc gatgatctgg
       61 tgaaacgagg tctgagtgag ggcctggagg atcgcgtgag cgtcctttac gtctcaccgc
      121 tgaaggcgct cagcaacgac atcgagaaga acttgcaaat gccgatcgag ggcattcgcg
      181 cgacgctgga agagctcggt tatggtgacc tcgagatccg gacggccgtg cgcacgggcg
      241 atacgccgtc ccacatgcgc acgaagatgg tgaagaagcc accgcagatc ttcgtcacga
      301 cgccggagtc gctctacatc ttgctcacca gcgaaagcgg tcggcgaatg ctctcggatg
      361 tgcgcaccgt gatcgtcgac gagatacacg cggtagtgac caacaaacgt ggcgcccacc
      421 tcgcgctctc gctcgagcga ctggaggctc tcgttgggcg ttcgatcacc cgggtcggtc
      481 tctcggcaac gcaaaacccg atcgaagacg tggcgcgctt cctcgtagga gtgcgcgagg
      541 acgaggcctc gatgcactgc cgcatcgtga acgtggggca tcagcgcgag atggacctgg
      601 ccgtcgagct tcccggctcc cctctcgaaa ccgtcatgtc tctagaggtc tgggaagaga
      661 tctatgcgag actcgcagcg ctcatcgaga cacacgagac gacgttgatc ttcgtcaact
      721 cgcggcgtct ggccgaaaga gccacgcgct acttgagcga gctgctgggc gaggagtcgg
      781 tgacctcaca tcacggtagc ctttcgcgta agcaccgctt ggacgcagag cgccgtttga
      841 aatcgggtga gctgaaggcc gtggtcgcga ccgcatcact cgagctcggg atcgacgtgg
      901 gcgacgtgga tctggtttgc caactcgggt ctccgcactc cgtggctacg tttctccagc
      961 gcgtcgggcg ctcgggtcac tggcacgggg ggacgcccaa gggacgcctt tttccgctga
     1021 gccgtgacga cctggtggaa tgcgcggcgc ttctgcgctg cgtccggcgc cgtgagctcg
     1081 accgaatcgc gattcccgaa ggaccgctcg acattcttgc ccagcagatc gtggcttcga
     1141 cggcagcgac cgagacgtgg agcgaggcgt cgctgcttgc tctggtccgg cgagcatggc
     1201 cctatcggaa cctcagcgaa gcatcgttcg ctcaggtcgt gcaggtcctt tcggagggct
     1261 tcgcgacgag ccggggccgg cgtggcgctt tcctccatcg cgatgaggtg aatcgacaaa
     1321 tccgggcgcg ccgcagcgcc cgtctcacgg cgatcaccaa cggcggcgcc atcccagaca
     1381 acttcgatta cgaagtacgc ctcgagccgg agggtcttcg agtagggtcg gtgaacgagg
     1441 acttcgccat cgagagcatg gccggcgaca tctttcaact cggcaacgtt tcgtatgaaa
     1501 tcttgcgcgt cgagccagga gtggtgcggg tcgccgacgc gaagggcaag ccgcctagcc
     1561 tgccgttctg gttcggagaa gcgccgtcca gggccgacgt tctttcaatc tcggtcaatg
     1621 cgatccgcga cggcatcgaa gctcgccacc aggatctcga ctcggtcgcg gaatggctcg
     1681 aacgcgaaac cggaattggg gagcttggag cgaagcaggt gacggactac ctggtcgcct
     1741 cacggatcgc gctaggcgcc atgcccacgc gggagaccgt cgtcctcgag cgtttcttcg
     1801 acgaaacggg cgcgatgcac ctcgttgtcc atgctccatt cggcagccgc atcaatcgcg
     1861 cctggggtct tgccctgcgc aaacgattct gccggagctt caacgtagag cttcaagcgg
     1921 ccgccaccga agacgcgatc gtcctctcgc tcggaccgac tcacagcttt ccgctcgaag
     1981 acgtcttccg gttcttgcgc gccgaaaacg tccgcggcgt tctgatccag gcgatgctag
     2041 acgctccgat gtttcagacg cggtggcgct ggaacgcaac cagggcgctt gcgattgcac
     2101 gatttcgcgg ggggaagaag gtgcctccac cctttcagcg aatgcaggca gacgacctgc
     2161 tcggcctctg cttcccggat caggtggcgt gtctcgagaa cgtagccggt gaccgggaga
     2221 tcccggacca ccctctcgtt cagcaaacca tcgaagactg tctccacgaa gcgatggaca
     2281 tcgacggcct ggaatctctt ctcgagcgcc tccatgccgg cgagctcgag ctcctcgcgc
     2341 gagacctcac cgagccgtcg ccgctggctc aagagattct caatgcacgg ccgtacgcgt
     2401 ttctggatga cgcacccctc gaagagcgtc gaactcaggc ggtcatggct cgaagatggc
     2461 tcgacccgac acgcgcatcg gacctcggcg cattggacca ggcggcgatc gaccaggtgc
     2521 ggaaggaggc atggcccgag ccacgcgacg ccgaagaaat gcacgactcg ctgctccttc
     2581 acaactgcct gatggagacc gacgccgcag acgctggctg ggaggcctgg ttggccgagc
     2641 tccgcgaagc aggacgcgcc acacgattca tcaccgaggg acatgtcgcg tggaccgccg
     2701 ccgaacgctt gcctttactc cagggcgcat ggccgagcgg ccgcgctgaa acgggggtcg
     2761 ccgttcccga gcgccatcgc cgggaatgct gggccaagga agatgccgtg cgtgagatcg
     2821 tgcgaggccg aattcaggcc tcggggccaa cgacgggtaa acggcttgcg gagatcttgg
     2881 gcctcgatgt ttcgctcgtg gacgccgccc tcgcggctct cgagggcgag gggttcgtcc
     2941 tccgcgggcg gttcacgcct gcttcgagtg ccgaggaatg gtgcgagcgc ggattgctcg
     3001 cgcggattca ccgctacacc ctcaaccgac tgcgcaagga gatcgaggcg gtttcggcag
     3061 cggacttcct gcgattccta gtcacttggc agcacgctgc acccgaggcc cgaatggagg
     3121 gtccggaagg gctcgcagcg atcatcgagc agctcgaggg aatcgaggcg ggcgccgctg
     3181 cctgggagag cgacattttg cccgtgcgca tggagggcta cgacccgagt tggctcgatc
     3241 agctttgcat ctgcgggcgc gtcacctgga gtcggcgcac accgcccctg ggacgagcgt
     3301 cgagccccat ccggacgagc ccgatcgcat tcactcgccg ggagcagacg gtcacttggc
     3361 gcttccgggc ctctggggag cagccggcat cgagtcgcgc aacgcaaact ctcgaaacgc
     3421 ttcgtcgatc cggtgcgagc ttcttcgccg acatcgtgag cgaaaccggt ctcctcacga
     3481 ccgaagccga gggcgcgctc ggtgaattgg tatctctcgg ccaggtcacg gcagatggat
     3541 tcaccggcct gcgcgcgctc cttgctccag atccaaagcg gccgcgagga ggacgacggg
     3601 cgctcgccgc gtacagcatg gaggcggcag ggcgatggag cgtccttccg gaagcgccgg
     3661 aagagcacga cgtggaaagc atcgcttggg cgctgctccg ccgctggggc gtggtctttc
     3721 gccgcgtcct cgatcgcgaa ggagaccttc caccctggta cgcgctgctc cgtgtttacc
     3781 gccgcttgga agctcagggc cgaatccgcg gaggtcgctt cgtggccggc tttgctggag
     3841 agcagtacgc actgcccgaa gccgtgaccg cgcttcgaaa gtcccgtcgt cgatcaagga
     3901 ccggcgagct cttgtccatc agcgcagccg atccgctcaa cctggtcggt attctgacgc
     3961 cgggtcagag gatcacagca acgccaaaga accgaatact cattcgcgac ggtgtcccga
     4021 tcgcgttccg tgagggaagc gagacgcact ttctcgaaga cccttcggat cagcgctggg
     4081 cgctcaccca ggcactgcaa aggcaatcgg tgccgcgagc ggtgcaggca tacctcggca
     4141 accggcccta aaatcgcatt cgcatgaaca tcttgttttg gctggaatac gctgcgctgg
     4201 atccttagag gccgacggaa aagcgtattg ccgctgcctc tgcgcccatc gtcgggctcg
     4261 tgagttgtct gcgccagccg cagtggtatg agcggactgc aagatgaccg atccgctcga
     4321 taagtgcttc tccgaggtaa cgatcaagag cctcaccctc cgcaaccggc tcatcaaggc
     4381 cgctaccttc gaggggaagt ctcccggcgg catcccgagc gaggcgctgg tgcgttttca
     4441 cgagcggatc ggccagggcg gcatcggcat gacgaccatc gcgtactgcg cagcggagcc
     4501 ggacgggcgc atccacgaga acatgatgta catgcacgaa gggattcgcc cggagctcga
     4561 gcgattcatc cgcaccgtgc aggccacggg ggccaaggta tcgggccagc tcggtcactg
     4621 cggcgccttc accaagaacc tcgagttcag cggtaagcgt ccactcggcc cgtccaaggg
     4681 caccaacgct ctcggcctcg catacggtct ctcgcgaata gccgaaatga cgaagcctca
     4741 aatccgggag cgagtccagg tcatcggccg cgccgccgcg ttcatgaaat cggtgggatt
     4801 cgatgcaatc gagattcact tcggccacgg ctacggcatc agccagttca tcagcccaaa
     4861 gaccaaccag cggaaggacg agtacggcgg gagtctccag aatcgaatgc ggttcgctct
     4921 cgaagtgctg gccgaggtcc gaaagagtgt cggcgacgat tatcccctgc tcggcaagat
     4981 cagcatgacg gacggagtgc ccggtggggt cacgtacgaa gactcggtcg agatcgccgc
     5041 catgctcgaa gcgggtggac tcgacgtcat catctgcagc ggcggcacga gcagcatgaa
     5101 cccgatgctg ctcttccgcg gagacagcat ggttccaggt ttgatcaagc acgagaagag
     5161 ccgtctcatg aagatcggca ttcggctcgc cgcaccgctg atgttcaagg actatcccta
     5221 tgaagagatg tacttcctcg aagaggcgca gcggattcgt gaccgcgtcc agtgcggcgt
     5281 ttgctacatc ggcggggtct gcacggccgc gagcattcgg accgtgatgg gggcagggtt
     5341 cgacttcatt cagttgggtc gcgggctgat cttcgatcct gacttgccga agcgcgccca
     5401 gtccgatgcc aactacgaga acggctgcaa tcactgcaac caatgcgcga cgctgatcga
     5461 agcgccgggc gggatctact gcgtcgagcg gcccg
//
LOCUS       DASRZF010000078         6651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1435736, whole genome shotgun sequence.
ACCESSION   DASRZF010000078 DASRZF010000000
VERSION     DASRZF010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 6651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6651
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1435736"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="VFG22_03325"
     CDS             <1..1038
                     /locus_tag="VFG22_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415303.1"
                     /translation="VVAWRRTHLPRMTAWPLAVDVPVESIAMPVTESDAAIPSGFRVL
                     PSPDGRPIVWSLLLIAMLILVKRRSIQARLGQRFLWVRAPWGVVVAITAFVVAIGQWL
                     GPNHLVCAIPLLAFAVHRPASWSPTIQRKNWGPAPARSLLDNEMSAGDLLDGTTFVGI
                     AALTAGAAALVVLAEPSAAIILLPLFFTGTRHHFHPTAAENTRMLYRFAADLRLPVDA
                     PEMSFGWERATEDGALRLRAHLATQRAGLLTLGFVVATSPVGFVSRRKVMLMVETRAQ
                     SDADDLMRRRVDVEPDLRAPDGSILRLVDWNAEAIELLRVLARKAPKPIKASRGTWLL
                     REISEPSRRAA"
     gene            complement(1044..1580)
                     /locus_tag="VFG22_03330"
     CDS             complement(1044..1580)
                     /locus_tag="VFG22_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415304.1"
                     /translation="MTPALIRFSQDLKTVLRIVEDPEIDDESRIAVAGGLLHVISTGG
                     AIPGERGVLRHLGDALLIRIVLSRVRERSPSAFEKHANQSPDMFEPLSEELAVMNAYL
                     GNRVTVLEKIAETFPTLNQQGHQAAACIEDPESSTWLYDSVHGALIDEIDFDEDDVVR
                     EMKQIDRILAPLASRVGR"
     gene            complement(1609..3627)
                     /locus_tag="VFG22_03335"
     CDS             complement(1609..3627)
                     /locus_tag="VFG22_03335"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET6415305.1"
                     /translation="MGRGWGCAWLCAFIVLGAAWSSRAAADDATLTEGGMDLHLFRPA
                     VDSRGYFSVNGSEVMPHKEFSFGLVLDAGFGILRYNGFVNDPDQSALDESRQSRIAKQ
                     AFTGTFMFSYGLFDRLVLGIQLPITFLRGDAVQTPDRGGSCLPGSANCLYNLEAGGLR
                     SQGVGDLTLVAKARLLNLGESPVGIAVTLRAGFPTAKSDQFAGEPGYSLWPTAVLEFQ
                     PIEKIRFALEGGYRWNSQRGATFIWDGRSNPANPNAAGATATDPVDVANAGHLFTYND
                     LITFGFGSAFRIASSLDFLIELYGTKIAQEIGTRGTLSMEVLGGFKYFVTNKSFLFIA
                     AGAGIPKTGFQAADIRATLGFIYEPAVGDRDKDGIPDDDDGCPDAAEDKDGFEDSDGC
                     PDLDNDGDGIFDVDDACPLVKEDYDGDRDEDGCPEGREGDRDGDGIPDDVDDCPDEPE
                     DFDGFQDDDGCPDLDNDGDGIPDERDLCPNDPEDFDGFEDEDGCPDVDNDSDRILDVD
                     DACPNDPETYNGYQDEDGCPDKGLVILEDTQITILEKVYFETDSAIIMARSFPLLDAV
                     AATLNASPQITLVEIQGHADERGSDEYNIKLTRARAASVMNALIERGVLKGRLRSAGY
                     GERCPVDPKGNAAAWEKNRRVEFKIIETDAGPTGVEVACPRGRSLVPR"
     gene            complement(3806..5017)
                     /locus_tag="VFG22_03340"
     CDS             complement(3806..5017)
                     /locus_tag="VFG22_03340"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET6415306.1"
                     /translation="MYKLVISDDEGHTTVVPLLRDEITIGRNEGNAIRLTERNVSRNH
                     ARLVKRNGAYIVEDLGSYNGVTVNGERIDAKSALEAGDQLGIGDYDLAFQSDVAAVAN
                     TLPSPKPRSEPPPRLVVLGEPAAGAEFTLNKPSLRIGRDERLDIWINHKSISHEHAEV
                     QLRDGKVTIYDLESANGMRVNGVRTSRAMLEAGDALELGEVRFRFLPPEGAHSLDPLP
                     EEALDTRRPSSSRKPLFVIALLGIVVLAGFGAVFATMTGTAEAPIAAKLAPAERAPAE
                     PAVSPEAVREGTRDEAGADADGARLDEPREWEGQLARARQAMADGRHDEAYVIANQLP
                     EDSVLRQTPEFAEIRYRYAQSHFNEGERALEEGDLEQARAEANLVLGLEGITPKQRRD
                     SKRLLRRAGQR"
     gene            5152..6084
                     /gene="mtnP"
                     /locus_tag="VFG22_03345"
     CDS             5152..6084
                     /gene="mtnP"
                     /locus_tag="VFG22_03345"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HET6415307.1"
                     /translation="MRPPSPSPGPRDPAHAPLADDGKAPRTLGVIGGSGLYQLESLTD
                     VEELDVTTPFGDPSDTLMTGTIGATRLLFLPRHGRNHRIPPHRVNYRANVMALKIAGA
                     EQILSVSAVGSMKENIHPGDMVIVDQFIDRTRHRLDTFFDDDGVVAHVAFAEPIDFAL
                     ASALGDAAKRAGASVHEGGTYLCIEGPQFSTHAESQLYRSWGASVIGMTNLPEARLAR
                     EAELPYATLAMATDYDCWHEAHEAVTVEAVIAVMQKNVAKAKEIILELSGKLPDPAGR
                     PATSALRGAIITNVDSISAAAREKLWPLIGKYVP"
     gene            6093..>6651
                     /locus_tag="VFG22_03350"
     CDS             6093..>6651
                     /locus_tag="VFG22_03350"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET6415308.1"
                     /translation="MSSLLVVGSVAFDTIHNHLGSHDRIIGGSATYASLAACQLAPVR
                     LVGVVGKDFPREAVDMLRSRGIDTTGLEVANGKTFHWEGRYSDDLSSRTTIQTDLNVF
                     ADFNPKIPAAFRDSEFVMLGNIAPQLQLHVLDQVDNPKLVVADTMNLWIDTALDALKE
                     LLRRVDILVINEEEARQLTGFHHPLA"
BASE COUNT         1225 a         2116 c         2003 g         1307 t
ORIGIN      
        1 gtcgtcgcat ggaggcgaac tcacctgccg cgaatgaccg catggccact tgcggtcgac
       61 gttccggtcg aatcgatcgc aatgccggtg acggagtcgg atgccgccat cccttcgggg
      121 tttcgagtcc tgccgagtcc tgacgggcgc ccgatcgtct ggtcgctgtt gctgatcgca
      181 atgctgatcc tcgtgaagcg aaggtccatc caggcgcgat tgggccaacg ctttctgtgg
      241 gttcgcgcgc cctggggcgt ggtcgtggcg atcacggcgt tcgtcgtcgc catcggtcaa
      301 tggcttggcc cgaaccatct cgtgtgtgcc atcccgctac tcgcgttcgc tgtacatcga
      361 ccagcaagct ggtcgccaac gattcagcga aagaactggg gtccggcacc agcaaggagt
      421 ctcttagaca acgagatgtc ggcaggcgac ctgctagacg gaacgacttt cgtggggatc
      481 gcagcgctga cggcgggggc ggcagccctc gtggtactgg ctgagccgag tgcggccatc
      541 atcttgttgc cactcttctt caccgggacg cgtcaccact ttcacccgac ggccgctgaa
      601 aacactcgga tgctgtatcg gttcgcggcc gatcttcgtc ttccagtcga cgctccggag
      661 atgtcattcg gttgggagcg tgcaacggag gacggggcgc tccgactccg cgctcacctc
      721 gcgacgcagc gcgccgggct cttgaccttg ggctttgtgg tcgcgaccag cccagttggc
      781 ttcgtgtcga gacgcaaagt catgctcatg gtcgaaacca gggcgcagtc cgacgcggac
      841 gacctgatga gacggcgcgt ggacgtcgaa cccgaccttc gggcaccgga cggcagcatt
      901 ttgagactag tcgactggaa cgcggaggcg atcgagcttc tccgcgtcct tgcccgcaaa
      961 gccccgaagc cgatcaaggc atcccggggc acctggctcc ttcgcgagat ctccgagccc
     1021 agccgcaggg ctgcctaaag cgttcagcga ccgacgcgag aagcgagcgg cgccagaatg
     1081 cggtcgatct gcttcatctc gcgcacgacg tcatcttcat cgaagtcgat ttcgtcgatc
     1141 agcgcgccat gaacggagtc gtagagccag gtgctcgatt ccggatcctc gatgcaggct
     1201 gccgcttgat gtccttgctg gttcaaggtt ggaaaggtct ctgcgatctt ttcgagcaca
     1261 gtgacgcggt ttcctagata ggcattcatg acagccagct cttcggacaa gggctcgaac
     1321 atgtccggcg actggttcgc gtgtttctca aacgcactcg gtgatcgctc acgaacacgg
     1381 ctcaagacga ttcggatcaa gagcgcatcg ccaaggtgac gcagcacacc acgttccccc
     1441 ggaatggctc cgcccgtgga gatcacgtgc agcaagcccc ctgcaaccgc aattcggctc
     1501 tcatcgtcga tctccggatc ttctacgatc cgcaggacgg tcttcaagtc ctgactgaat
     1561 cggatcagtg caggggtcat tagctctatg aagcgctcgc gcgacatatc acctcggcac
     1621 cagagaacgg ccccgtgggc atgctacctc aactcccgtc ggaccggcgt cggtttcgat
     1681 gatcttgaac tccacgcgcc gatttttctc ccaagcagca gcattgccct tcggatcgac
     1741 cgggcagcgt tcgccgtatc cggcagacct caaccgaccc ttgagcactc cccgctcgat
     1801 gagagcgttc atgaccgaag cggctcgggc ccgcgtgagc ttgatgttgt actcgtcgct
     1861 accgcgctcg tcggcatggc cctgaatctc gacgagcgtg atctgcgggc tggcattgag
     1921 ggttgcagcg accgcatcga gtagcggaaa gcttcgcgcc atgatgatcg cactgtcggt
     1981 ctcgaagtag accttctcga gaatggtgat ctgggtatcc tccagaatca cgagaccctt
     2041 gtcggggcac ccgtcctcat cctggtagcc gttgtaggtc tctggatcgt tcgggcaagc
     2101 atcgtcgacg tcgagaattc ggtcgctgtc gttgtcgacg tcggggcaac catcctcgtc
     2161 ctcgaatccg tcgaaatcct ctgggtcgtt gggacaaaga tccctctcat cgggaatccc
     2221 atcgccgtcg ttgtctagat ccggacagcc gtcgtcgtct tggaagccgt cgaagtcctc
     2281 gggctcatcg ggacagtcgt cgacatcgtc gggaattcca tcaccatcac ggtcgccttc
     2341 cctgccctcc gggcatccgt cctcgtcgcg gtccccgtcg tagtcttcct ttacgagagg
     2401 gcacgcgtcg tcgacgtcga agataccgtc accatcgttg tcgagatccg gacagccgtc
     2461 gctgtcctcg aatccgtctt tgtcctcggc agcatccggg cacccgtcat cgtcgtccgg
     2521 aatgccgtcc ttgtcgcggt cgcccacagc cggctcgtag atgaatccga gcgtcgcgcg
     2581 aatgtcggca gcctgaaagc cggtctttgg aatcccggcg ccagcagcga tgaaaaggaa
     2641 gctcttgttc gtgacaaagt acttgaagcc cccaagcacc tccatcgaaa gggtgcctct
     2701 cgtcccaatt tcctgggcga ttttggttcc atagagctcg atgagaaagt cgagcgagga
     2761 cgcaatacgg aaggcggagc cgaagccgaa ggtgattaag tcgttgtaag tgaacaggtg
     2821 accggcgttg gctacgtcga ccgggtcggt tgcggttgca ccggcggcgt tcgggttggc
     2881 gggattcgac cggccatccc agatgaaggt agcgccgcgc tgggagttcc agcgatagcc
     2941 accttcgaga gcaaaccgaa tcttttcgat tggctggaat tccagcacgg ccgtcggcca
     3001 aagcgagtag ccgggctcac ccgcaaactg atcgctcttg gcggtaggga accctgcgcg
     3061 gagggtgacc gcaatgccga ccgggctttc tccgaggttg agcaggcgcg ccttggcgac
     3121 cagcgtgagg tcgccgactc cctgagaccg caaaccaccg gcctctagat tgtagagaca
     3181 gttcgcagac ccaggcagac aacttccccc gcgatccggc gtttgcaccg cgtcaccgcg
     3241 aaggaacgtg atcggaagct ggatgccgag gaccagccga tcgaacagtc cgtagctgaa
     3301 catgaacgtc ccggtgaacg cttgtttcgc gatccgagac tgacgggatt cgtcgagagc
     3361 cgactggtcc gggtcgttca cgaagccgtt gtaacgaagg atgccaaagc cggcgtcgag
     3421 gactagaccg aagctgaact ccttgtgcgg catgacctcg ctgccgttga cgctgaagta
     3481 gcctcgcgag tcgacggccg gacgaaagag gtggaggtcc atccccccct cggtgagcgt
     3541 tgcatcgtcg gccgccgccc tactcgacca cgcggcgccg agcacgatga acgcgcaaag
     3601 ccacgcgcac ccccagccgc gacccacgga tgcgctccgc caatgatgtg ttcccccaaa
     3661 caccctgtct cagtctgtca tgggcgacgc ccattaatca acgattctgc gaccctgcgt
     3721 cggtgttcgg tcaacaattg atatgacttc ggggcggaaa gcgggtccaa gggccgacga
     3781 gcctcgatga gatcggcgag cccccttacc gctgaccggc gcgccgcagc agccgcttgc
     3841 tgtcccggcg ctgtttcggc gtgatgcctt cgagcccgag caccaaattc gcttcggctc
     3901 gagcctgttc gagatcgcct tcctcgaggg cgcgttcacc ctcgttgaag tgtgactgcg
     3961 cgtagcgata tcggatctca gcgaactcgg gcgtctggcg gagaacgcta tcctccggga
     4021 gctgattggc gatgacatac gcctcgtcgt gacggccatc tgccatcgct tgccgagcac
     4081 gggcgagctg cccttcccac tctcgaggtt cgtccagtct tgcgccatcg gcgtccgctc
     4141 ccgcttcgtc tcgcgtgcct tcgcgcacgg cctccggcga cacggccggc tcggcgggtg
     4201 cgcgctcggc aggtgcgagc ttggcagcga ttggtgcttc agcggtgccg gtcatggtcg
     4261 cgaacaccgc accgaagcct gcgagaacga cgatgccgag cagggcaatc acgaatagtg
     4321 gctttcgcga cgaggaaggc ctgcgggtgt cgagcgcctc ctctggaaga ggatccaaac
     4381 tgtgtgcacc ttccggcggg agaaagcgaa aacgaacttc gccgagctcc aaggcatcgc
     4441 ccgcctcgag catggcccgg gaggtccgaa ccccattgac tcgcatcccg ttggcgcttt
     4501 cgagatcgta gatcgttact tttccgtctc gcagctgcac ctctgcgtgc tcgtgggaaa
     4561 tcgacttgtg attgatccag atatcgaggc gctcgtctcg tccgatgcga aggcttggtt
     4621 tgttgagcgt aaactccgcg cctgccgctg gctcaccgag gacgaccagt ctcggcggcg
     4681 gttcgctccg gggctttggg gacggcaggg tattggccac ggccgcaacg tcggattgaa
     4741 aggcgagatc gtagtcgccg attccgagct gatcacccgc ctcgagcgcc gacttcgcgt
     4801 cgattcgttc accgttgacc gtgacgccgt tgtaggagcc caggtcctcg acaatgtaag
     4861 ctccattacg tttcacgagt cgcgcatgat tccgagacac gttgcgttcg gtcagccgaa
     4921 tcgcgttgcc ctcgttccgg ccgatcgtga tctcgtcgcg gagaagcggt acgacggtcg
     4981 tgtgaccttc gtcgtccgag atgacgagct tatacatctt gtcgacccaa gggcagtttc
     5041 attcgcagtt tcgcggggtt gagagcatgg tactccggcg ggctggggtg tcaatctcgt
     5101 ctgcgcgagg cttggcaccc ggcgagaacc cggctatgcc cgtccccgaa gatgaggccc
     5161 ccttccccaa gccccggccc gcgtgacccg gcccacgcgc ccctcgctga cgatgggaaa
     5221 gccccgcgaa cactcggcgt catcggggga agcggcctct atcaactcga gagcctgacc
     5281 gacgtagagg agctcgacgt cacgactcct ttcggagatc cgtccgacac gctcatgacc
     5341 gggaccatcg gcgcgacccg tctcctgttc ctcccccgcc atggtcggaa tcatcggatc
     5401 ccaccgcatc gggtgaacta ccgcgcaaac gtgatggcat tgaagatcgc cggcgcagag
     5461 cagatcctca gcgtgtccgc agtgggttcg atgaaagaga acatccatcc aggagacatg
     5521 gtcatcgtcg accaattcat cgatcgaacc cggcatcgtc tcgacacgtt tttcgatgat
     5581 gacggtgtcg tggcccatgt cgcgttcgcc gaaccgatcg acttcgctct cgcgagcgcc
     5641 ctgggggacg cggccaaacg tgccggggcc tcggtgcacg aaggcggtac ctacctgtgc
     5701 atagaaggcc cgcaattttc gacgcatgcg gagtcgcagc tctaccgatc ctggggcgcg
     5761 tcggtgatcg gcatgacgaa tttgccggag gcccgtctcg cacgagaagc cgagctgccc
     5821 tacgcgacgc tcgcgatggc gaccgactat gactgctggc atgaggctca cgaagcggtc
     5881 acagtcgaag cagtcatcgc ggtcatgcag aaaaacgtcg caaaggccaa ggaaatcatc
     5941 ctagagctct ccgggaagct ccctgatccc gcgggacgcc cggcgacgtc cgccctccgt
     6001 ggcgccatca tcacgaacgt cgattcgatc tcggcggccg cacgtgaaaa gctgtggccg
     6061 ctcatcggca aatacgtacc ctgaaaggca acatgtcctc ccttctcgtc gtcggttcgg
     6121 tcgctttcga caccattcac aaccatctcg gctcgcacga tcgaattatc ggcggctcag
     6181 ctacctacgc gtcgctcgcg gcgtgccagc tcgcacccgt tcgcctcgtc ggcgtggtcg
     6241 gcaaggattt cccacgcgag gcggtcgaca tgctccgcag ccgcgggatc gacacgacgg
     6301 gcctcgaagt cgcgaatggc aagaccttcc attgggaggg acgctattcg gacgacctct
     6361 cgtcacgcac gaccattcaa accgatttga atgtctttgc cgatttcaat ccaaagattc
     6421 cagccgcctt tcgcgattcc gagttcgtga tgctcgggaa catcgcccct cagctgcagt
     6481 tgcacgtgct cgaccaagtc gacaacccca agctggtggt cgccgatacg atgaaccttt
     6541 ggatcgacac cgccttggac gcactcaagg agctcctgcg gcgagtcgac attctcgtca
     6601 tcaatgaaga ggaggcacgg cagctgaccg ggttccatca ccccttggcc g
//
LOCUS       DASRZF010000079         5134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2464810, whole genome shotgun sequence.
ACCESSION   DASRZF010000079 DASRZF010000000
VERSION     DASRZF010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 5134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5134
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2464810"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="VFG22_03355"
     CDS             complement(<1..992)
                     /locus_tag="VFG22_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415309.1"
                     /translation="MLSASRTTYAGLIGVILVYLCLALLHVWIVRIYQPGDEKRHTKY
                     VVVIETSDRLPTVEETRAATHPPLYYALVAKTVMRGVESTEGIEGAVRAARCVSVFFG
                     AVALLFAFLIIRLLLPNHPALAVHTTGIMAVIPSYSNNCAVLGNDSLSLASQFAMIYL
                     ALLILLRGPSWPRCLILALTLSIVALSRVSGVLVMPVALFAVAAGTWWNVEGSPRRRA
                     LLATAIPMTLIAWVAATSGWFYGGNISASGDLTGQSELLQQVRNHPVRSPFAVMLDPR
                     KWLEIHDETWGRLAGSIFIRGSLEGLARAVTIVSLLGAAIALWQVHVWERMCDWR"
     gene            1072..2040
                     /locus_tag="VFG22_03360"
     CDS             1072..2040
                     /locus_tag="VFG22_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HET6415310.1"
                     /translation="MKGGSNWLRFLVRLTGPALLILVIWSLDDRAALWEAVRHASWPP
                     LAAAIALIIPVIHLKVARWRGLLAARGFAYPLRRTYAAVLPSLYLGMLTPGRVGDALR
                     IQYVHREIDVPYSDGLATTLMDRFSDLYVVALVAAFGLVHFASILRGDLAQATWVAVA
                     VALIAPLLLMVRGPADLFGWVLRRLTDRWHESYRNVLRSMRSLVWKGLTLAIPLTAAA
                     FAINYVQGWLIARAIGVDLSYLDVASLLSATSLLGLMPISIAGVGVRELFLALVFPAL
                     GLPAAQGVAFGLLIFVCINLSTVLVGFVAWQIAPPPVGASEPDASF"
     gene            2219..2536
                     /locus_tag="VFG22_03365"
     CDS             2219..2536
                     /locus_tag="VFG22_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415311.1"
                     /translation="MTKTYDDIAAANTQCPTVITGASEITINMPSWIGCRTRRWMSGV
                     RNSEWGTGRPAKLREDRLVLPFETLDSAEVVEQLRLLEVFIGGLQACAVLVPRTFVQR
                     LIG"
     gene            complement(2833..3201)
                     /locus_tag="VFG22_03370"
     CDS             complement(2833..3201)
                     /locus_tag="VFG22_03370"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET6415312.1"
                     /translation="MEEMRRHLGPAADKWIKSIKEISPEFAKVNVEFPFGELYTRDVL
                     DDKTRELCTVAALTVQGFALPELKVHVKGAINCGASREEVLEVITQMIAYCGFPAATN
                     GLLAAKEVLDELGAEPADKP"
     gene            3752..3940
                     /locus_tag="VFG22_03375"
     CDS             3752..3940
                     /locus_tag="VFG22_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415313.1"
                     /translation="MDFNPDCGASLVSNEQIPIVDGAFNYELTGSGTASGTIDGETAS
                     GEAMVGDCSTTWEATPSP"
     gene            4058..4330
                     /locus_tag="VFG22_03380"
     CDS             4058..4330
                     /locus_tag="VFG22_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415314.1"
                     /translation="MERIEHEVDGSLHVPIDRESADAFARERIVVRAGIELENAEAAS
                     RQQCAIWVSALAIAANSSGFVIPRRAMTTRIRSDTSCRSSRVSWLT"
     gene            4497..>5134
                     /locus_tag="VFG22_03385"
     CDS             4497..>5134
                     /locus_tag="VFG22_03385"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6415315.1"
                     /translation="MRFVLRRGALGVLLIAVPLGSAGRAVADSAYEEAFRRSLVLEAG
                     GNPAAAAAVLELLVDSHPDDYVLFLQLGWLYFVLEDFKNAEAHYARASTLSEGGAEPE
                     LGLAWVYLRTNRPDEALEAFDRVLAMRPEDASALEGRALAADAARSLRFYVDGNVSLL
                     LYPGNPFKAWAIALASSLDARIARRMLVGATYRYAAYAMRDTGTGARAGLRAG"
BASE COUNT          911 a         1602 c         1580 g         1041 t
ORIGIN      
        1 cgccaatcgc acatccgttc ccagacgtgc acttgccaga gcgcgatggc agcgcccagg
       61 agcgaaacga tcgtaacagc cctcgcgagg ccttccaggc ttccccggat gaagatcgac
      121 cccgcgaggc ggccccaagt ctcatcgtga atctccagcc attttctcgg atcgagcatg
      181 accgcgaacg gcgatcgaac cgggtgattt cgcacctgct ggagcagctc ggactggccg
      241 gtgaggtccc ccgaggccga gatgttcccc ccgtagaacc agccggatgt ggctgcgacc
      301 caggctatca gcgtcatcgg aattgccgtg gcaaggagcg ctcgtcgacg cggggagccc
      361 tcgacgttcc accatgtccc ggcggcgacc gcgaacaagg ccaccggcat gacgagcacg
      421 cccgataccc ggctgagagc gacgatggag agagtgaggg cgagaattag gcagcgcggc
      481 cagctcggac ctcgcagaag gatcagaaga gccaagtaga tcatcgcaaa ctgcgatgcc
      541 agcgatagcg aatcgttgcc gagcaccgca cagttgttcg agtatgacgg aatgactgcc
      601 atgattccgg tcgtatgaac ggccaacgcc gggtggttcg gtagcaacag ccgaatgatt
      661 agaaacgcga agagcaaagc aacggcaccg aaaaagacgg acacgcagcg cgctgctcgg
      721 actgcgcctt cgatcccttc ggtcgactcg acgccccgca tcacggtctt cgctaccaag
      781 gcgtagtaga gaggcgggtg cgtggctgcg cgcgtttctt cgaccgttgg caggcgatcg
      841 ctcgtttcga tcaccaccac gtacttcgtg tgacgcttct cgtctcccgg ttgatagatc
      901 ctcacaatcc acacgtgcag cagcgcgagg cacagataca ccagaatcac tccaatgagg
      961 ccagcgtatg tcgtgcggga agcgctcagc atgatccgcc ctataccgtc gaatgcggag
     1021 tttgggaatt tgggcgctcg atcgccgcgc cgtgtatagt cctgcgcgcc gatgaaaggt
     1081 ggttccaact ggctgcgttt tctggtccgg ctgaccggcc ccgcgctgct gatcctggtc
     1141 atctggtcgt tggacgatag ggcggcgctc tgggaagcgg tgagacacgc gagctggccg
     1201 ccccttgcag cagcgatcgc tctcatcatt ccggtcatcc acttgaaggt ggcgcgttgg
     1261 cgagggcttc tcgccgcgag gggctttgcg taccctttgc ggcgcaccta tgcagccgtg
     1321 ttgccgtctc tctacctggg catgctgact ccaggtcgcg tcggcgatgc actccgcatc
     1381 cagtacgtgc atcgggaaat cgatgtccca tacagcgatg ggcttgccac caccttgatg
     1441 gatcgattca gcgatctata cgtcgtagct ttggtagctg cgttcggtct ggtccacttc
     1501 gcatcaatcc ttcggggaga tctggcgcag gcaacctggg tcgcagtagc ggtcgccctc
     1561 attgcgcctt tgttactgat ggtgagaggg cctgccgatc tcttcggttg ggtgctgcgt
     1621 cggctcaccg atcgctggca tgagagctac cgaaacgtac ttcgctcgat gcgttcgctg
     1681 gtgtggaagg gtctgacgct cgcgattcct ctcaccgctg cggcttttgc catcaattac
     1741 gtgcagggtt ggctgatcgc gcgggccatc ggcgtggacc tgagctacct cgacgtcgcg
     1801 agcttgctgt ccgccacgag cctgctcggc ctgatgccga tctcgatcgc aggcgtcgga
     1861 gttcgtgagc tgttcttggc gctggtcttt cccgcgctcg gactaccggc tgcacaaggc
     1921 gttgcgtttg ggctgttgat tttcgtctgc atcaacctct cgaccgtgtt ggtcgggttc
     1981 gtcgcgtggc agatcgcgcc tccccccgtc ggcgcaagcg agcccgacgc atcattctag
     2041 ggcaaccggc ttcttgccac gcatcccgcg acggcgtgct cggtttcgtt gcgacgatgc
     2101 tttgttcaag gggtggggca gatgtccaga ggcgcggaag caccttcgga acgctcttcc
     2161 gatacgcgag atagcgctcg ccgaactggc gtacgaggtc gcgctcttcg aaaagaggat
     2221 gacgaagacg tatgacgaca ttgccgccgc gaacacgcag tgcccaacgg tcatcaccgg
     2281 cgcctccgaa attacgatca atatgcccag ttggatcggg tgccggacca gacgatggat
     2341 gtctggggtg cgaaactccg aatggggaac cggtcggccc gcgaagctcc gcgaagatcg
     2401 cctcgtgctc ccttttgaga cgctggattc ggccgaggtc gttgaacaac tgcgcctact
     2461 cgaggtcttc atcggtggtc tgcaagcgtg cgcggtactc gttccgcgca cgttcgttca
     2521 gcgcctcatc ggctgatagt cgacataaac gccctttcag gttcgcgtcc caggcgacta
     2581 acattaagta ccgcgaaagt cagtcctcag agtggtcccc gtgggccaag agagctccaa
     2641 cgacgagcag gttgcgagtt gttccatcgg cattcaatta tccaagagaa agagggtgct
     2701 cggggctggt tctgatcttc tcgtcgaccc gagcacacgt aacgctgcgc cctttattcg
     2761 cccttcagcc acgttcaagc tcccagtttc aacatgaggg atgcgtggct ccgagccaac
     2821 tagccgcgcg acctagggtt tgtccgccgg ctccgctccc agctcgtcga ggacttcctt
     2881 cgcggccagc aagccgttag tggcagcggg gaatccacaa taggcaatca tttgcgtgat
     2941 gacttctaag acctcctcgc gcgaagcgcc gcagttgatg gcgcctttga cgtggacttt
     3001 gagctcgggg agagcgaagc cctgcaccgt caacgccgct actgtgcaca gctctcgggt
     3061 tttatcgtcg agcacatcgc gtgtgtacag ctcgccgaac ggaaattcga cgttcacttt
     3121 cgcgaactca ggcgagatct ctttgatgct cttgatccac ttgtctgcgg ccgggccgag
     3181 atggcggcgc atttcttcca ttccctcttt gtactttgcc gttgtcatcc gcttcccttc
     3241 ctcgtgaaat cagccgccac cgctggcgtt ggagaaacct ttccgttccg tcagtagggc
     3301 gtcccgtcct tgtgtgggtc gagaccgagc ttcgcccccg agtagcgcct tggactgaac
     3361 ttcatgtcga tatcggagca ctcgccgtga gtataatagc tacagacgcc ctttcaggtt
     3421 cacgccacat cacgattagt accgtgcaaa tcggccctcg acatggtccc catctaagca
     3481 tcgggcgtcg catcacggac caaagcgtcg gctccttttg cgcaaccgcg cactatctgc
     3541 gtctcgcctg gcaggcaccg cgggcgccgg cggagcagcg ggtggggtgg ggaacgggcg
     3601 ggaccggtgg ggtggagccg atcacctccg gttcgtggac gggctccagc gatcaaggtc
     3661 cagacgaaac gttcacgatc tgtttcaacg tgagtgagga cggaaccgag gtcgtgacag
     3721 ggggcttggg ctgcatgggc attcactcgc gttggacttc aacccggact gcggtgccag
     3781 cttggtctcg aacgagcaga ttcccatcgt cgacggggcc ttcaactacg agctcacagg
     3841 tagcggaacc gcgagtggca ccatcgacgg cgaaaccgcg tcgggcgaag ccatggtggg
     3901 agattgttcg accacgtggg aggcgacgcc cagtccctga gcggaccagg cgcctgtctg
     3961 tccgccccat gaggttcgat gagccgctgc cttcggcgga ttttcacgaa tcgataggcg
     4021 gtttccggtc gcgtggaaac atgacgtaca ccgagcgttg gagcgcatcg agcacgaagt
     4081 cgacggcagc ctccatgttc ccatcgatcg agagagtgcg gatgccttcg cgcgcgagcg
     4141 tatcgtcgta cgtgccggca tcgaactgga aaatgccgag gccgcatcac gccagcagtg
     4201 cgccatatgg gtctccgcgc tagcgattgc ggcaaacagc agcgggttcg tgatcccgcg
     4261 tcgcgcgatg accacgcgta ttcgctcaga cacatcctgc cgctcgtcga gagtgagctg
     4321 gctgacctga cctcgaacca tcgcatcgct gaccacttgt ccggcagatc gggccagttg
     4381 tctgaagctg caaacgcatg gcgcagatcg acgtacccct gcgccttcat tcttcctgcg
     4441 ctctctactc cacccggctt cgttgcccgt ccccaaagcg agcgtatatt ctgctggtgc
     4501 gattcgtact tcgacgcggc gcgctcggcg tcttgttgat cgctgtcccc ctggggtccg
     4561 cgggacgcgc cgtcgccgac tccgcgtatg aagaggcctt tcgacgctcc ctcgtgctcg
     4621 aggcgggtgg caatcccgcc gcggcggccg ccgttctcga gttgctcgtg gactcccatc
     4681 cggacgacta cgtcctcttc ctccagctgg gttggctcta tttcgtgctc gaagacttca
     4741 agaacgccga agcccattat gcgcgagcga gcaccctctc ggagggaggt gcggagcctg
     4801 agctcgggct cgcctgggtc tatctacgga ccaatcggcc cgacgaagcc ctcgaggcct
     4861 tcgaccgggt cctcgcgatg cgaccggagg atgcctcggc gctcgagggg cgcgcccttg
     4921 ccgccgacgc ggcccgctca ctgcgtttct acgtcgacgg gaacgtctcc ctcctcctct
     4981 accccggaaa tcccttcaag gcatgggcga tcgcactcgc ttcttcgctc gacgcgcgga
     5041 tcgcgcgacg gatgctggtg ggcgcgacgt atcgatacgc ggcgtacgcg atgagggaca
     5101 cgggtaccgg ggcccgggcc gggctaaggg ccgg
//
LOCUS       DASRZF010000080        30066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4186362, whole genome shotgun sequence.
ACCESSION   DASRZF010000080 DASRZF010000000
VERSION     DASRZF010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 30066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30066
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4186362"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1332)
                     /locus_tag="VFG22_03390"
     CDS             complement(<1..1332)
                     /locus_tag="VFG22_03390"
                     /inference="COORDINATES: protein motif:HMM:NF023649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HET6415316.1"
                     /translation="MKRSPCLSDALLLSFTVGLLSGCAPSGGPPPYATTGALGAEPTE
                     VFWGDTHLHTSYSPDAYFFGNTTADPDTAYRYAKGRPVIHPYHGGKIQIGTPLDFLVV
                     ADHAEMMGVPFRLFRGDAELTKTASGKRFIEMVEAGKNQEVFFEFITAINKNQPFEDL
                     NGEDLRRSVWSDMVAITERHNEPGRFTSFIGWEWTSTPDGKNLHRVVFMPEGGDTAAK
                     FIPYSAFDSDKPEDLWAWLEETSSRTGAHFTAIPHNSNISGGLMFNDVDSEGRPITAE
                     YARTRMKWERVIEVTQIKGDSETDPILSPNDEFADFEPFNHLIDTEAVREGHEGPKPQ
                     AGDFARAALGRGLETEAKVGVNPFKFGMIGSTDSHTGMSSAEEDNFHGKTAFDSTPAN
                     TFDTFLDIKGFGADMSAAGVAGVWAEENGRTPLFEAFQRKEVYATTGPRVRL"
     gene            1505..2944
                     /locus_tag="VFG22_03395"
     CDS             1505..2944
                     /locus_tag="VFG22_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415317.1"
                     /translation="MPVPAAAQLGPDGSTITSSDYAIDLSRGVVIGTSRVTGLSGAAT
                     ALAEGVEGGLQNPSAVAYRGPQWPDWYDYWLALGTTYPFKTGDFYNSGRVVGDSGRLA
                     NDSIFFLIPGAYFQMWNLGVGLTIDAQFVKIEGAIDPVTMERDTLRLRFTVFHIQAGY
                     GFLDGQLIIGGGLRILRQRTYLSSRLPSEGDSYKTLGLGAELGVMFKPHHKAWSLGAS
                     LFPELSTGVRERQDGSQTSDGDIVVGDFYVPRSTRIPTVGSVGFSYQFGLRPSNPPWV
                     SAEQLAAHDLERLDDRKRAAEEDERDEIARVRARGGPEVEICIQAVKQTYARRYAEIK
                     RSRKELKNLAWEMLRREYRQGWPRRYYLLSADLQINGRVDNAVGVESFIFQTVQRSGE
                     RVTVSPRIGFETEVWPQRMKLRGGSYLEPSRFAESELRIHGTFGLDIKLFKWNVFGLW
                     PDDYRWQLTGAVDFARDYGAFSLGIGGWY"
     gene            3059..3982
                     /locus_tag="VFG22_03400"
     CDS             3059..3982
                     /locus_tag="VFG22_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415318.1"
                     /translation="MKNIVLSACVLAALVSLGCKKNKTTPPPPELVVTLVSPGESPLE
                     PLRYAIPPGTVVDSLLEISDIQTTAETTEESLGAFGTLPGFKLWLHAGPTVMQPKGAR
                     YVLRITQAEPVLPAGVTDRQSEEVDQGVEALRGMRGRFDLNEQGIVIDADVPWSEGQE
                     RIHPRVAIMIGNIRSAIATVPVPKEPVGIGAIWEVQRPLRIWSARVTQITRYQLTDRA
                     GDRFRVAVTVHQTAAPQVADLNPELEMHVRGYELHARGHVLSDLGLPLALEASLESDS
                     AADIALVSPEKTEPLTSERRSVLRLASKIER"
     gene            3988..5016
                     /locus_tag="VFG22_03405"
     CDS             3988..5016
                     /locus_tag="VFG22_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415319.1"
                     /translation="MVRILLTAALLLATVACGPKYQPQSVHVADPGPPQRVLPGIRVV
                     DPGMTPRAPLRYRVPPGHTELLFVEIAQAEVVRAEGKGEEAGMPPIQIEVKMGPAEPT
                     PQGLMRHPVQITQVRISEMAEQMSPAQREQFEKTLAPLLEVQGWSEMDIQGRIRRGEF
                     SGMEDVSPRLRTMLGNIRSALLTIPFPDEPLGPRARWEVERKLQFSGVWVDQVATYEI
                     EKMDRDQLTLQITARQSAPTQTMASGRLEAYQASMIGSAVVRLDKFTPTSEAEATAQM
                     RISTQTQAGPELVQVDERTMVKLYPADQAKESGRPDAKKSNEPDENAPPPADPSVLID
                     PGKQKLKW"
     gene            complement(5038..5769)
                     /locus_tag="VFG22_03410"
     CDS             complement(5038..5769)
                     /locus_tag="VFG22_03410"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HET6415320.1"
                     /translation="MTRTAAALIIGNEILSGKIIDTNTTFLARGLFELGIELRRVVVC
                     PDEVQTISEDLTTLRNGHDLVFTSGGVGPTHDDVTIDGVAASFGRPVVRSEAVEKMIR
                     HYYGDRATEAHYRMANMPEGAEMIRSSDAPWPTVVIENVFVLPGVPEIFELKFTDLRK
                     RLDRGQGFHSEAIYTLSGEGEIASLLERIAKDFPGVMVGSYIKWKAEDYRTKVTFDGN
                     DRETVARAADTLVAELDPALFVRRG"
     gene            complement(5771..6901)
                     /locus_tag="VFG22_03415"
     CDS             complement(5771..6901)
                     /locus_tag="VFG22_03415"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HET6415321.1"
                     /translation="MTDDNLAREHEEEQTRRVLVLNGGGDAPGLNAVLRAFVKTATEL
                     GLEVYGSEDGFAGLLEEPCRVVKLSRADVRGILPKGGSVLGCSDHSDPFAYEVQSADG
                     TKRRVDFSDRVLNKLEELAIDILVLVGGGRTMNLGMRFAKLGVAVVGVPKAIGNDLAA
                     TDLTFGLDTAVSTATTAIDALHSASEAHGSVMILEVMGREAGWIALMSGIAGGADVIL
                     IPEIHYDIDRVVHKIDSRSSSDENFSIIVIGEGSKPLGGTVSRIGAAQEQGSDWSEGP
                     GFQLARLLEGRIDHEIRVTVLGHLQRGGSPSAFDRILGTRFGVHAAELCFHDNTGQVV
                     ALRGQEVTSVALESAIANPKRVPPDGELVGVARAVGIELGQE"
     gene            complement(6891..7625)
                     /gene="rlmB"
                     /locus_tag="VFG22_03420"
     CDS             complement(6891..7625)
                     /gene="rlmB"
                     /locus_tag="VFG22_03420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HET6415322.1"
                     /translation="MTRVLAGRRPVLEALRGNARVLRIFTEPQRPYPEVREAADALGI
                     PVANRERAELDRLSDGVRHQGVVALADDYQFVEVEEIIAGADGPPALVALDEVTDPQN
                     LGAIIRSAVTLGLDGLIIPKHRAAGITAAVVRASAGATEHAAIARVTNLQRTLLSLSE
                     SGIEIIGLDAGADIDLRELEPSSTGRALVVGSEGQGLRRMVRQRCDIVVHVPQRGPMD
                     SLNASVAAAIAMYEMTCKRRRTSDDG"
     gene            complement(7622..8329)
                     /gene="pyrF"
                     /locus_tag="VFG22_03425"
     CDS             complement(7622..8329)
                     /gene="pyrF"
                     /locus_tag="VFG22_03425"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HET6415323.1"
                     /translation="MNENDPRSHIALALDVPDLDAAKALIEQTREHLDTFKVGLELFT
                     AVGPAAIEAIHAADARCFLDLKLHDIPATMGRAVARAASMGVAYLTVHGAAGPESLRK
                     AHENAGSTRLLAVTVLTSLDAAALAAIGLTGTPESAANRLAKLAWNAGLRGFVASAQE
                     SKSLRGELGEEAFLVTPGIRPAGADRGDQKRVMTPAEAIEAGADLLVVGRPIRDARDP
                     SAAAAEIARQVALALSR"
     gene            complement(8359..9099)
                     /locus_tag="VFG22_03430"
     CDS             complement(8359..9099)
                     /locus_tag="VFG22_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415324.1"
                     /translation="MSEEKESSVLFNLRELMSIEEDRVRGEEEERLQREAAERERIAA
                     EARARQEEEQARLRAKEEARRAEERLAREEEERAQREREEAELRVRLEQEAREKAEAH
                     ARMLDHERELATISAQQRKGVHPGLFAAAAILVLGLGAGSYFGIYKPAVERREATALA
                     AKMEAERAAREAEEARAAAELEAQKRAEAEAEQARLAEMARREAQAAAAARRAAAPSA
                     PSAPANRRGSSRQRKADTDDPLAGLDSL"
     gene            9310..10959
                     /locus_tag="VFG22_03435"
     CDS             9310..10959
                     /locus_tag="VFG22_03435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HET6415325.1"
                     /translation="MGELQNFLQYLERGDVSEIVFQSGAAAAAKKKGTLKPVTREPLS
                     AKHILKLVSNTPLEVLVPASDGATEPTAIRIDGIGYIATMARSGEMLMLRVRREGKHT
                     IPPKVPIGAIQIAPVRVPDLQPETSQPELSQPEIRKPELSRPEVQRSYRPAPEASPVA
                     PFVPAGVALQASSELRALLAAARNKPASDVHLMSGEPARFRSSGYLVPEGQALSDQEV
                     TALVMPLLDARNAAQLDELGYADFACDVEGAGRLRVNVSKQRSGIKGCFRLIMPEPPT
                     LESLGLPPELRQMLDYHQGLVVISGPSGAGKTTTLAALVDLFNAERAVHIITVEDPVE
                     IMHPKKKAIVTQREVGAHTQSFHTAFKGSLREDPDVIAIGELRDRETVEKALEAAETG
                     HLVFATMSTPSGASTIDRLIDMFPPDDQSQVRATLAGALKFVVSQRLVPRKDGKGRVA
                     AVELLTGGMALWSLIRDDKLFQLPSLLQRGRGFGMIRIEDSLNALVATGTVTVETAKI
                     FADDARLIGQVPAPGSGAGAPESASNRRKGNVLNPFAKKDS"
     gene            10963..12048
                     /locus_tag="VFG22_03440"
     CDS             10963..12048
                     /locus_tag="VFG22_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HET6415326.1"
                     /translation="MPALDQYFDVLLNKGGSDLHLSVGYPPTIRLKGELVPIAEQPLD
                     RLTIKNLMDELLDQNQRDHFHAYHDLDFAYGYEDKARFRANYLYKTTGPGAVFRTIPS
                     EILSAQDLGLPAAVVELANRRSGLVLVTGPTGSGKSTTLAAMIDHVNRTREGHILTIE
                     DPVEFVHRPRKCQITHREVGIHTPSFADAIRSAGRENADVILVGELRGRETMKMALQL
                     ASFGILIFATVHTNSAPATIDRFVNAFPASQQPQIRGMLADSLAGIVAQQLLRTADGT
                     GRVAAQEILIGTPGVASAIRESKTSMIQSLIQGGGSYGMQSMDAVLGRMVADRKIRAE
                     DALEKASDKISFVKLPLVAAALKTALS"
     gene            complement(12041..13876)
                     /locus_tag="VFG22_03445"
     CDS             complement(12041..13876)
                     /locus_tag="VFG22_03445"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET6415327.1"
                     /translation="MPEGIASTRPEPSGLHPGAVVGGRFEVIDQIDEDPQAVIWSAKD
                     QQTKRAILVHVIRPDLVSVETAPDFREACRSAANLSHRNIARVFGVGRTPRGEHFVAG
                     EWIDGTRLSEFIKERDQKGEPLSLKGVYNVIAHLCSALSYAHGHGPHGTLRPSIVWVT
                     RGGRVKIRDFGVGQVLLGTAGPNVFKNADHACLAPEVKRGEPATLRSDVFGIGAILYE
                     LLTTRSPGSNFVAPSHVRDDIPAELDGILFQCLAPDPKDRFESPEAVRNALLPFVSAA
                     NSVPPAADADLSLDIDLGSVLPPADLEIPGVTIHAQAPLAPPPPPRRGLRSLIGELSA
                     DPKQRWMVARDKMDHGPFNARELIERLNIGGFSGDDLTFNIDTGERKPLREWKEFREL
                     VLQREHQDTSAAEQAARVNAHRSEKRSSAMKWLIAAAVIGVTIISVGGFLLSRSSDIR
                     EAASTELDDLFKLGKISTGEAGLLPDLPVRKRKRGSTAPAPARRSGGFYSYEEAMSQA
                     MEIGDASQSGGEGRLTGNQVASVMNQNLNRFYSRCVIPELKSGGKPGSVKVDLAIAGD
                     GSVLGATIHGGSSGFQGCMGQQIKSVRFPGFGAPRMGARYSFTVN"
     gene            complement(13927..15600)
                     /locus_tag="VFG22_03450"
     CDS             complement(13927..15600)
                     /locus_tag="VFG22_03450"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET6415328.1"
                     /translation="MTEPPEEEGIQTFAELGLHEDVLKAIEEMGWDTPTPVQRDTYPL
                     AVAGHDIIVQSRTGTGKTGAFGIPLVDRLMSRDGDEQALVLAPTRELALQSAREIAKL
                     GARRGVDTVAVYGGASMEKQVEALKKGARVISGTPGRVLDHLKRGTMDASRLRVLVLD
                     EADEMLSMGFAKELHAIIERLPKKRQTLLFSATVDRAIQRVAEKHMHDPQFLGLSGDH
                     VGALGIEHFVYMVSGVGRVANLISVLEIEDPESALIFCNTKAETEEVSAALQRAGFNA
                     EWLNGDRPQREREEVLGRVRREELRFLVATDVAARGIDVSHLTHVINFSLPTNVEQYV
                     HRTGRTGRAGRTGTAITLVGPTELGILYYLRIQYRIFPVERTLPSEGEQRTRQEADRI
                     ALIEVAYPNAPTERELALVRRFMTHENAERMLAGLLAAFFGTESEDLDKVAATRRRRG
                     KQHVDEHSNGGKKKKRRTQDEDEDDDEGPTATLCVNVGRRDELKASELGRLMRERTGL
                     KRKEIGRIRVRDRHSLVDVPEDRVEDVIESMAGAILHDRELELELDKAD"
     gene            complement(15597..16052)
                     /locus_tag="VFG22_03455"
     CDS             complement(15597..16052)
                     /locus_tag="VFG22_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415329.1"
                     /translation="MPKKEQRGGLGRRLAIGIYAIALSYLVVIGFASVIPAVFWPPAD
                     DSFDLECADGLRLLRHEVDELRLAYLATNETDVAPLERALKSWDLRLNALAKRCDEDQ
                     VHLLNQYRHRVELGLERYMREDAPLANRVSEALGSKADYPSPEPPEPTP"
     gene            complement(16119..16259)
                     /locus_tag="VFG22_03460"
     CDS             complement(16119..16259)
                     /locus_tag="VFG22_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415330.1"
                     /translation="MGTGVTTFLWYGLCGGVVALTIVVGVGLFFWGTHREKRKRRNPP
                     PS"
     gene            complement(16264..16959)
                     /locus_tag="VFG22_03465"
     CDS             complement(16264..16959)
                     /locus_tag="VFG22_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6415331.1"
                     /translation="MSEDGRGILLVEDDPALALGLSDSLRFEGYRVVHTAKGAKAIEL
                     AASEDVDCVILDLMLPDMNGYQVCETIRRGDPILPILMLTARGQESDKIRGLEAGADD
                     YVTKPFAIGELVARIRALFRRAGRSVRPSQAEIRIGNINIETGSQTLTRGSDTMHLSF
                     YEVELLKFLWEREGEPVSRDEILEKIWGLEAGPNNRTVDNFIVKLRRKIEPKPDRPIH
                     ILTVYGVGYKLVC"
     gene            complement(17007..18557)
                     /locus_tag="VFG22_03470"
     CDS             complement(17007..18557)
                     /locus_tag="VFG22_03470"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET6415332.1"
                     /translation="MEGGRFRLVYFILVPAILVAAAILGYYTIRSSRDYAQLGEQTIA
                     QSLLSVAEQRVERIEGYITVADHTVFRLVNPNDSSTIDTAWLPQAATQTPSVRSVLLL
                     DPDGNIIQWASREDKKDRRAFLRVFRQDLLKSLELDAAEPNLLRHLHQQVDDEGYLLS
                     YRAYATLAGQVTFIVAVHDTKYLVDEVFPAMFVSDETTPLYNIVDESNQIIFGPDLSE
                     AGVYTVDHRFPRTLYRWRLQVAPRQAARLQARGRSRMQGELTLPIMSFAIILLSAIFF
                     IYAAEKERRLNQLKSELMANVSHELKTPLSVVRMFADMLRSGRVPSDARRLEYLDIIC
                     RESERLTSLIDNVLDFSALEGGKGPYRLKRGNLGDAIGRALEAFRYRSEQDGVEIQLN
                     ASEDLPQVLMDQEAIALAVINLLDNAVKYGEQSPVELTVTSKRNMVDVAVRDHGPGIP
                     SDSLRRVFERFYRGPTSTQTRGSGIGLSLVKHIAQAHGGRAWAKNAQGGGAVVGFSIP
                     VAAGKALG"
     gene            complement(18656..21097)
                     /gene="lon"
                     /locus_tag="VFG22_03475"
     CDS             complement(18656..21097)
                     /gene="lon"
                     /locus_tag="VFG22_03475"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HET6415333.1"
                     /translation="MSDEEDTITSIPPPDSGEIEFGSELPVLPIRNAVLFPAAVAPFD
                     VGREKSVALVEDIENLDQPIIAIIAQRDPSTDDPDQSDLYPVGVAARVLKALKHSSGN
                     YSLILQGLVRIRLEQVVTSEPYMRARVSRLDEPSVEDVESEALALSLRDIAKQVIQLM
                     PELPREAGSLIDSIQEHGQLADLVAANLDAPVEEKSQLLETLDAKERIRKVLRLLTRQ
                     LEILKMRERINSQIKEEMGKNQREYVLRQQLKAIKEELGEEEGDQGDLDILEERIAKA
                     NLPGEADTVAHKQLKRLRQMQVGSAEYTVVRTYIDWILDIPWSKQTADNMDIAAVRKV
                     LDEDHSGLEKVKKRIVEYLAVRKLKTDKKGPILCLIGPPGVGKTSLGRSVARALGRKF
                     HRISLGGVHDEAAIRGHRRTYVGALPGQVIQGMKKASTINPVFMLDEIDKIGHDFRGD
                     PAAALLEVLDPEQNDTFSDHYLEIPYDLSKVMFIATANVGDTIPAPLRDRMEIIEIPG
                     YTRREKADIARVHLVPKQLDEHGIKPEQVTLEDGAIAAIIDHYTREAGVRNLERQIAS
                     VIRGVAVKVAEGEEGPWIISDEDSLKPYLGAPRFSSEVAERTAETGVATGLAWTSVGG
                     EILFIECTKMKGTGKLQLTGQLGDVMKESAQTAMSYVRTRAKSFGIPEDFLETSDIHI
                     HIPAGAMPKDGPSAGVTMMTAIVSLLTGIHVRHDVAMTGEITLRGRVLPVGGIKEKVL
                     AAHRAGIKRVILPERNTADLDEVPEEVRETLEFVPVSKMDAVLANALLDPDRLKLDLS
                     DPRPDKGEGVQPSAP"
     gene            21274..22017
                     /locus_tag="VFG22_03480"
     CDS             21274..22017
                     /locus_tag="VFG22_03480"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET6415334.1"
                     /translation="MRLSVVVPCFNEEGNIAPVVAQAVSVGRRLASELEIIVVDDGST
                     DETAMVLDRLRTSVPELQIVAHPRNLGYGAAVRSGLDRALMDYVFLTDGDGQFDLEEL
                     PQAIRLLGEHDVVVGYRTARQDGWWRRLWGRSWTALVNLVFGLRVRDANCAFKLVPQS
                     LLRSSRLRSQGALISAELIFEAQRSDLRVGECAVSHYPRQAGRQTGASLRVIATAFFE
                     LAACMPRRWAPEKRLGPHRGSRLEQSRFR"
     gene            complement(22025..22927)
                     /locus_tag="VFG22_03485"
     CDS             complement(22025..22927)
                     /locus_tag="VFG22_03485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET6415335.1"
                     /translation="MPFKDQTTSASQTVLTQVGEARTAPRGSENGCLVVIYGPEMGRR
                     VRVSTEPVIIGRSPKCEIQIDQESVSRNHCRIGFEEGEFVVRDLGSTNGTYVNDNLVG
                     DEGRLRHGDQLRVGRTILKFIVGDDVEVEYHEEIYRLMTTDGLTQLNNKRYFDEMLDR
                     EVARAKRYKRSFSLLVFDIDHFKKINDRFGHLAGDTILRQLGAMLLGRLRVNDVLARI
                     GGEEFALITPEVGVDGAKELAGKINRLISDTRFDFDGTRVDVTVSVGVAEWQTHYEDP
                     SDLYKAADEKMYEAKRTGRNRVCF"
     gene            23168..24241
                     /locus_tag="VFG22_03490"
     CDS             23168..24241
                     /locus_tag="VFG22_03490"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HET6415336.1"
                     /translation="MLSPPFTDVDIAKAKPGSGSWVVPRSDGTRAVLTVDGELQSYIQ
                     TLFSRYDVPAGGLVAIEPKSGRVLAYVGYEAGKGNSPQVVTDSGPPAASVFKIITASA
                     LLDAGVKSSTSVCYGGGMRGLEQVDLIDNPSRDRWCATMADALGYSINAVFAKLADRN
                     IDEARMTQYVSAFGFGQRLPFDLPTIPSPVDVPDSRLERARMAAGFWHSQLSPLHGAL
                     LAATIANDGKMPYAALVERIESQTGETLYEHTPRTFRQVVPRQTARLAGEMMTRTVTK
                     GTSKKAFWDPKGRPFLPGIAVAGKTGTLSRYEPHRTYNWWVGYAPVDDPKIAVAALVV
                     NEPKWRIKASYVAREALRQYLMR"
     gene            24340..25152
                     /locus_tag="VFG22_03495"
     CDS             24340..25152
                     /locus_tag="VFG22_03495"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET6415337.1"
                     /translation="MFHSIRLGALAALTAISLLGASAATAQSPVLSRVIKTDTLRVGM
                     SANQPPLNMKSKSGQMIGLEVDLAKGLAYALGFELEIVQKPFGELLGALKKGKVDMVM
                     SGVDITAARSQDFLFVGPYLLSGKSLVTTSKALAGAETVDDINTPNVTFVTLKNSTSQ
                     AFVKKNLPKAKSVTVDDYEAAVGMVIDGKASAMIADLPACALAVLRNPDAGLVTLAEP
                     LSVEPMGIAVSAKDPRFYNLIENYIDAIEATGILSGLREKWLEDPSWLDELP"
     gene            complement(25579..26322)
                     /locus_tag="VFG22_03500"
     CDS             complement(25579..26322)
                     /locus_tag="VFG22_03500"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HET6415338.1"
                     /translation="MDTAMRDLERIEERSSDEGRGVLVLSGVGLVAAVGIVVAAVAAL
                     PSGDEARPEDLLQMLAIADETEAHEAGGERAEDPDIDPESLSFPTTLVTEERAEVAAA
                     MAAAAAELAHLDPLTSPAPRSDIAAGLPAAVTAGPDRTLVEMAAIRDPLVAATVTERT
                     QSSPAGYEGRYTLQVISYGDAEEARVFASALRKRGHAAYVTSGSVEGRGMHWRVRIGP
                     FETRQQAQDYRTTFEREEGMNTFIVRNKG"
     gene            26488..29067
                     /gene="gyrA"
                     /locus_tag="VFG22_03505"
     CDS             26488..29067
                     /gene="gyrA"
                     /locus_tag="VFG22_03505"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HET6415339.1"
                     /translation="MAESSQKHPVALEDEMRRSYLDYAMSVIIGRAIPDVRDGLKPVH
                     RRILYSMHEQKVHWNSSYRKSARIVGDVLGKYHPHGDTAVYDALVRMAQDFSMRYPLV
                     DGQGNFGSVDGDPAAAMRYTEVRMHRLASELLADIEKDTVDFGPNFDDSEREPLVLPS
                     RIPNLLINGSGGIAVGMATNIPPHNLREVVDATVRLMGDPELTVDELMQDDDEAGRLG
                     VKGPDFPTAGYIYGRAGIHLGYRTGRGRIVMRARATIESLPGKGDREMIVITEIPYQV
                     NKAELLKKVADLVRDKRIEGISDIRDESDRDGLRVVIELKRDAHGEIVLNKLYQMTAL
                     QSTFGYNCLAIVGQRPEVLDLRTALLRFIDHRREVVVRRTRYDLAQAEAQRELVEGLG
                     MAVTDVDLVVSTIRSSKDPDEARERLMKLPLTGLEEFVRRAGRPESEIQAAKEHGEYL
                     LSERQAKAILEMRLSRLTGLEREKLATEYGSLCDVIKELQAILASKKLLDEVIVTELD
                     EIRERFGDARRTEIVEAEGEISIEDLVPDEEVVVTVSHAGYVKRVPLSEYRSQGRGGK
                     GLRAMDTRDKDFVSWVFVVNAHANVLFLSDKGKAYLKKVYEIPETSRAARGRAVVNLV
                     GMEPDERVAAILPIREFVEDGYLLTCTRRGRVKRTSLGAYENIRQTGIIGVAIGEGDG
                     LLTARIVTPGQHVLIGTSHGMSIRFPIEDVRAMGRDSMGVKGIDLREGDFVIGMDIIE
                     DEGEQQVLTVSANGYGKRTKVPEWRIQNRGGKGIIAMDTSERNGALVKLRLVSPDDQL
                     MVITNGGQVIRTHVGEIRETGRNTQGVRVIRLGEGEQVVDVEPVAEGEDDSAEESPET
                     EEN"
     gene            29159..>30066
                     /locus_tag="VFG22_03510"
     CDS             29159..>30066
                     /locus_tag="VFG22_03510"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HET6415340.1"
                     /translation="MSNGPVIGIDLGTTNSVVAVADQPGGRVLAESDGAKLIPSVVSF
                     HPSGDILVGAAAKERRLLDAQNTVYSVKRLIGRPYTSYEVKQAQERFAFELSEGPSGG
                     VLVTARGETYTLSEISAFVLRHVRKVAEEALGQECSQAVVTVPANFNELQRSATKAAG
                     RVAGLDVLRILNEPTAAALAYGYNKTSTERVAVYDLGGGTFDITILQLAGDVFEVLAT
                     AGDTFLGGDDVDTSIADQMAAQFLEQNRYDARQDRQAYERLRAAAEWAKIQLSDKPDV
                     KLCIEELAYGEGGVALNLDYALTRQQL"
BASE COUNT         5510 a         9347 c         9377 g         5832 t
ORIGIN      
        1 cagccgtact cggggtcccg tggtcgcgta cacctctttg cgttggaagg cctcgaagag
       61 cggagtgcga ccgttctcct cagcccagac tcccgcgaca cctgcggccg acatgtcagc
      121 tccaaagcct ttgatgtcga gaaaggtgtc gaaggtgttt gcgggggtcg agtcgaacgc
      181 cgtcttgccg tggaagttgt cttcctctgc ggaggacata cctgtgtgcg aatccgtgga
      241 gccgatcatc ccgaacttga agggattgac gccgactttt gcctcggttt ccaggccgcg
      301 accaagcgca gcgcgcgcga agtcgcccgc ttgaggtttc ggcccctcgt ggccttctcg
      361 caccgcctcg gtgtcgatca agtgattgaa gggctcgaaa tcggcgaact cgtcattcgg
      421 cgaaaggatg gggtccgttt cggagtcgcc cttgatctgc gtcacctcga tcacgcgttc
      481 ccatttcatc ctggttcgtg cgtactccgc cgtaatggga cggccttcgc tgtccacgtc
      541 gttgaacatc agtccgccac tgatattcga gttgtgcgga atggcggtga aatgagcgcc
      601 cgttcgcgaa gaggtttctt cgagccaggc ccagaggtcc tcgggtttgt cgctgtcgaa
      661 cgcgctgtac ggaatgaact tcgcagcggt gtcgccgccc tcaggcatga acacgacacg
      721 gtggaggttc ttgccgtcgg gggtcgaagt ccactcccag ccgatgaacg atgtgaacct
      781 gcccggctca ttgtgacgtt ccgtgatggc gaccatgtca ctccaaaccg agcgccgcag
      841 gtcctcgccg ttcaagtcct caaacggttg gttcttgttg atcgcggtga tgaactcgaa
      901 gaacacctct tggttcttgc cagcctcgac catctcgatg aagcgcttcc cgctcgccgt
      961 cttggtgagc tccgcgtccc cgcgaaagag gcgaaacggg acgcccatca tctctgcgtg
     1021 gtccgcgacg acgaggaaat cgagcggtgt cccgatttga atcttgcccc cgtgatacgg
     1081 atgaattacc ggacgcccct tggcgtagcg gtaagcggtg tctgggtccg ccgttgtgtt
     1141 gccgaagaaa taggcgtccg gcgaatagct cgtatgcaga tgggtatcgc cccaaaagac
     1201 ctcagtcggc tcggcgccaa gggcgccagt ggttgcatac ggagggggcc ccccgctagg
     1261 cgcgcagccg gacaagagcc cgactgtaaa agagagcagg agcgcatcgg acaaacatgg
     1321 ggatcgcttc atcatcgttc cttcgcgaag gccggagcct agcatgcagc cccgtctgcg
     1381 tcgggcccga cgattcgctg gtgaagtccg cgccgtccct gtgtagcctc caggatggat
     1441 ggccgtgaag acccaaggca cagggcgcgc tctgatttgc ttcgtgctat tggtattgag
     1501 tgggatgcca gtacccgccg ccgctcaact cgggcccgac ggtagcacca tcacgtcctc
     1561 cgattacgcg atcgatctca gccgcggcgt ggtcatcggc acttctcgcg tcacgggctt
     1621 gtccggcgca gcaaccgctc tggccgaagg cgtggaggga ggtctacaga acccctccgc
     1681 ggtggcttat cgcggtcctc aatggccgga ttggtacgac tactggctgg ctctcggcac
     1741 gacttatccg ttcaagacgg gggacttcta caacagcgga agggtagtcg gagattcggg
     1801 acggctcgcc aatgacagca tcttcttctt gatccccggc gcctatttcc agatgtggaa
     1861 cctcggcgtc ggtctcacca tcgatgcgca gttcgtcaag atcgaaggtg caatcgatcc
     1921 cgtcaccatg gagcgggata cgctccggct gcggtttaca gtctttcaca ttcaggccgg
     1981 ttatggcttt ctggacggtc agctgatcat cggaggaggc ctccgcatct tacgacagcg
     2041 cacctatctc tcctcgcgac tgccgagcga gggggacagt tataagacgc tcggcctcgg
     2101 tgcggagctc ggcgtgatgt tcaagccaca ccacaaggcc tggagcctcg gcgcctcgct
     2161 ctttcccgag ctctctacgg gggttcgtga gcgtcaggac ggcagtcaga cttctgacgg
     2221 cgacatcgtg gtcggcgatt tctacgttcc gcgttcgacg cggatcccga ccgtcgggag
     2281 tgttggattc tcgtaccagt ttggcttgag gccctcgaac ccaccttggg tcagcgccga
     2341 gcagctcgct gcgcacgatc tcgaacgcct cgatgatcgg aagcgtgccg cagaggaaga
     2401 cgagcgggac gagatcgctc gggttcgcgc gcgcggggga cccgaagtcg agatctgcat
     2461 ccaggccgtc aagcaaacat acgcacgcag gtacgccgag atcaaacggt cccgcaagga
     2521 gttgaagaac ctagcctggg agatgctgcg acgtgagtac cggcaaggct ggcctcgccg
     2581 ctattatttg ctcagcgccg acctccagat caatggccgc gtcgacaatg ctgtcggggt
     2641 cgaatcgttc atcttccaaa cggtgcagcg ttccggcgag cgagtgacgg tctcgccccg
     2701 aatcggcttt gaaaccgagg tctggcccca gcgcatgaag cttcgcggag gatcgtactt
     2761 ggagccgagc cgcttcgccg aatcggagct ccgaatccac ggaacgtttg ggctggatat
     2821 caagctcttc aagtggaacg tattcgggct ctggcccgat gactatcgct ggcagctcac
     2881 cggggcggtc gacttcgcca gggactatgg ggccttttcc ttaggtatcg gcggctggta
     2941 ctagtcatcg cgcgttcgcg ccggcgctct tcgatacgcc tattgggacc ggtccgcgct
     3001 tgccctgagt ccgattcctc tctagaagga ccggagcaat gagggacaac gccagcaaat
     3061 gaagaatatc gtcttgtccg cgtgcgttct tgcggcgctc gtttcactgg gttgcaagaa
     3121 gaacaagacc acgcctccgc ctcccgagct ggtggtcacg ctagtcagcc caggcgaatc
     3181 gcccctcgaa ccgctgcgct acgcaatccc gcccggcacc gtcgtcgatt cgctgctcga
     3241 aatcagcgat atccagacca cggccgagac gacggaggag tcgctgggtg cgtttggtac
     3301 cttgccgggg ttcaaactct ggcttcacgc cggaccgacg gtcatgcagc ccaaaggtgc
     3361 tcgctacgtc ttgcgcatca cccaggcaga accggtgctt cccgccggcg tgaccgacag
     3421 acagtccgaa gaggtcgacc aaggcgttga agcgcttcgg ggcatgcgcg ggcgcttcga
     3481 cctcaacgag caagggatcg tgatcgacgc agacgttccc tggtccgagg gtcaggaacg
     3541 cattcaccca cgtgtcgcca tcatgatcgg gaacatccgc tcggctatcg cgacggttcc
     3601 cgtcccgaaa gagccggtag ggattggagc gatctgggaa gtgcaacggc ccctacgtat
     3661 ttggagcgcc agagtcactc agatcacgcg ctaccagctc acggatcgcg ccggcgatcg
     3721 gttccgagtc gccgtgaccg tgcatcagac ggcagcgcct caggttgccg atctcaatcc
     3781 agagctcgag atgcacgttc gcggatacga gctgcacgcg cgcggacacg tgctctccga
     3841 cctcggcctt ccactggcgc tcgaagcatc cctcgaatcc gattcagccg ccgacatcgc
     3901 actcgtctct ccagaaaaga cagagccttt gacgtccgag cggcgctcgg ttctgcgatt
     3961 ggcctcgaag atcgaaaggt aagatccatg gttcgtatcc tgttgaccgc tgcgctcttg
     4021 ttggcgaccg tcgcgtgcgg cccgaagtat cagccacagt cggtgcacgt tgccgacccc
     4081 ggacccccgc aacgcgttct tccgggtata cgcgtcgtcg atccaggcat gacccctcgc
     4141 gcgccacttc gatatcgcgt acctccgggc cacaccgaat tgctcttcgt cgagatcgct
     4201 caagccgagg tcgttcgcgc ggagggtaag ggcgaggaag ccggcatgcc cccgattcaa
     4261 atcgaggtga aaatgggacc cgcggagccg accccccagg gtctcatgcg tcatccggtt
     4321 cagattaccc aggtgcgaat ttcagagatg gctgagcaga tgagcccggc tcagcgcgag
     4381 cagttcgaga agacgctggc accgctactc gaagtgcaag gctggagcga gatggacatt
     4441 caggggcgga ttcgccgagg cgagttcagc ggaatggaag atgtatcgcc aagactgcgc
     4501 acgatgctgg gcaacatccg ctctgcgctt ctcacgatac cgttcccgga cgagcccctc
     4561 gggccacgtg cgcgctggga agtcgagcgc aagcttcagt tctcgggggt ttgggtggac
     4621 caggtcgcga cctacgagat cgagaagatg gatcgggacc agctcaccct tcagatcacg
     4681 gctcgccaaa gcgctcccac ccagacgatg gcaagcggtc gactcgaagc gtatcaggcg
     4741 tcgatgattg gttccgccgt ggtgcgattg gataagttca ctccgacctc agaagccgaa
     4801 gccaccgctc agatgcgcat cagcacgcag acccaggcgg gtccagagct ggtgcaggtc
     4861 gacgagagaa cgatggtgaa gctctatccc gccgatcagg ccaaagagag tggcagaccc
     4921 gatgcgaaga agtcgaacga gccggatgag aacgctccgc cccccgcgga tcccagtgtc
     4981 ctcatcgacc ccggcaaaca gaagctcaaa tggtgagagg cgcgccgtcc gcgtgcctca
     5041 gcctctgcga acgaataggg ccgggtcgag ctctgcaacg agtgtgtccg cggccctggc
     5101 caccgtctct cggtcgtttc catcgaacgt gaccttcgtt cggtaatcct cggctttcca
     5161 tttgatgtaa ctgccgacca tgacgcccgg aaagtctttg gcgatgcgtt cgagcaggct
     5221 tgcgatctcg ccttcgccgc tgagcgtgta gatcgcctcg gaatgaaatc cttggccgcg
     5281 gtcgaggcgc tttcgaaggt cagtgaactt gagctcgaaa atctctggaa cgcccggtag
     5341 cacgaacacg ttctcgatga cgaccgttgg ccaaggggcg tccgagctcc gtatcatctc
     5401 tgccccctcc ggcatgttgg ccatgcgata gtgggcttcc gtggctcgat cgccgtagta
     5461 atggcggatc attttttcga ctgcctcgga acggacaacc gggcgaccga acgaagccgc
     5521 aacgccatcg atggtgacgt catcgtgcgt ggggcctact ccgccgctgg tgaagaccag
     5581 gtcgtgaccg ttgcgaaggg tggtgaggtc ctcgctaatc gtctgcactt cgtcggggca
     5641 gaccaccacc cgccgcaact cgattcccag ctcgaagagc cctcgcgcga ggaacgtcgt
     5701 gttcgtatcg atgatctttc cggagaggat ttcgtttccg atgatcagtg cggctgcagt
     5761 tcgagtcatg tcactcctgg cctagctcga ttccgacggc ccgcgccacc ccaaccagct
     5821 cgccatccgg tggcacgcgc ttggggttcg caatcgcaga ctccagagcc accgaggtga
     5881 cttcctggcc tctcagcgcc accacctgac cggtgttgtc gtgaaagcaa agctccgcgg
     5941 cgtgaacccc aaagcgggtg ccaaggattc ggtcgaacgc ggagggagag cctccccgct
     6001 gcaggtgtcc gagaacggtc actcgaattt cgtgatcgat tcgcccttcg aggagacgcg
     6061 ctagctgaaa gccagggccc tcgctccagt cggacccctg ttcctgagca gcgccaattc
     6121 gggaaacggt tccgccgagt ggtttgctgc cttcgccgat gacgatgatg ctgaagttct
     6181 cgtcgctcga ggaacgagaa tcgattttgt ggacgacgcg atcgatgtcg tagtggatct
     6241 cggggatcag aatcacgtca gctccaccgg caatccccga catgagcgca atccagccag
     6301 cctcacgccc catcacctcc aagatcatca cgctgccatg tgcctcgctg gctgagtgca
     6361 gtgcgtcgat ggccgtggtc gccgtcgaaa ccgcggtgtc gagcccgaac gtgaggtccg
     6421 tggcagccaa gtcgttgccg atcgcctttg gaaccccgac cacggcaacg cccaacttgg
     6481 cgaaccgcat gccgagattc atcgttctgc ctccgcccac gagcactaga atgtcgatcg
     6541 cgagctcttc gagcttgttc aagacccggt ccgagaagtc gacccgtctc ttggtaccgt
     6601 cggcgctctg cacttcgtaa gcgaaggggt cactgtgatc ggagcagccg agaacgctgc
     6661 cgcctttcgg cagaatgcct ctcacgtcgg cccggctgag cttcacgacc cggcagggct
     6721 cttcgaggag tccagcaaat ccgtcctccg atccgtacac ctcgaggccg agctcggtgg
     6781 cagttttgac gaacgcacgg agcactgcgt tgagacccgg agcgtctccc cctccattga
     6841 gcacgaggac tcggcgggtc tgttcttctt cgtgctctcg ggcgaggttg tcatccgtca
     6901 tcggaggtcc gccgtcgttt gcaagtcatc tcgtacatcg caatggccgc ggccaccgac
     6961 gcgttgagcg agtccattgg acccctttgt ggaacgtgaa ccacgatatc acagcgttgt
     7021 ctaaccatgc ggcgaaggcc ctgaccctcg gaacctacca ccagggcacg tccagtcgag
     7081 cttggctcga gctctcgaag gtcgatgtcg gcccccgcat cgagaccgat gatctcgatc
     7141 ccggattcgg agagagagag gagagtccgt tggaggttgg ttactctggc gatcgctgca
     7201 tgctcggtgg cacccgccga agcgcgaacg acggccgccg tgatcccggc tgcccgatgc
     7261 ttcggaatga tcaagccgtc gagtccgagc gtcacggcgc ttcggatgat cgcaccgagg
     7321 ttctgtgggt cggtgacttc gtcgagtgca accagggcag gggggccatc ggctccggcg
     7381 atgatctctt ccacttcgac gaactgatag tcgtccgcca acgcaacgac tccctggtgt
     7441 cggacgccgt cgctcagtcg atcgagctcc gcgcgttccc gattggcgac cgggatgccg
     7501 agagcgtctg cggcttcccg gacctccggg tatgggcgct gcggctcggt gaatatccgg
     7561 agaaccctcg cgtttcctcg caaggcctcg agaacgggcc gccgacctgc gaggacccgt
     7621 gtcatctgga aagtgccagc gcgacctggc gcgcgatctc ggccgccgcc gcgctcggat
     7681 cgcgcgcgtc tcgaatgggc cgtcccacca cgagcaggtc ggcgcccgcc tcaatcgctt
     7741 cggcgggcgt catgactcgt ttctgatcac cgcggtccgc gcccgcgggt cgaattccgg
     7801 gcgtgacgag gaacgcctct tcaccgagct cgccgcgaag acttttggac tcctgagccg
     7861 acgcgacgaa gccacgcagt ccagcgttcc aggcgagctt cgcgagtcga ttcgccgcgc
     7921 tctctggagt ccccgtcaat ccgatggccg cgagcgctgc cgcgtccagg ctcgtgagga
     7981 ccgtgaccgc gagaagtctc gtcgaccccg cgttctcgtg cgccttccgc aacgattcgg
     8041 gccctgccgc tccgtgtacc gtgaggtacg cgactcccat cgacgcagct cgtgcgactg
     8101 cacgccccat ggtcgctgga atgtcgtgca acttgaggtc gaggaagcac cgagcgtccg
     8161 cggcatggat ggcctcgatg gcggcaggtc ccacggcggt gaatagctcg aggcccacct
     8221 tgaacgtgtc gaggtgctct cgcgtttgct cgatcagcgc tttcgcagcg tcgaggtcgg
     8281 gaacatcgag ggcgagggcg atgtgcgacc tcgggtcgtt ctcattcatg agtcaccgca
     8341 gcaacgctac ggtgcggact agaggctgtc cagtccagcc aagggatcgt cggtatcggc
     8401 ctttcgctgc cgcgagctgc ctcgccgatt cgcgggagca ctcggagcgc tcggagctgc
     8461 ggctcggcgc gccgccgctg ctgcttgagc ttcgcgccta gccatctctg ccagtcgagc
     8521 ttgctccgcc tctgcttcgg cacgcttctg ggcttccagc tccgctgcgg cacgggcttc
     8581 ctcggcttcg cgcgcagcgc gctccgcttc catcttggcg gccaacgccg tagcctcacg
     8641 gcgctcgacc gcgggcttgt aaattccaaa atacgatcct gcgccaagac cgaggaccaa
     8701 gatcgcagcc gctgcgaata ggccgggatg aacgcccttg cgctgctgcg cgcttatggt
     8761 ggcgagctcg cgttcgtgat cgagcatgcg cgcatgggcc tcagccttct cgcgcgcttc
     8821 ctgctccaag cggacccgaa gctcggcctc ttcgcgctca cgctgggccc gctcttcctc
     8881 ttcccgcgcc aaccgctctt cggctcgacg cgcttcctct ttggcgcgca accgggcctg
     8941 ttcctcttcc tgccgggcgc gtgcctcggc ggcaatgcgc tcgcgctcgg ccgcctcgcg
     9001 ttgaagtcgc tcctcttctt cgccacgaac acggtcttct tcgatgctca tgagctcgcg
     9061 aaggttgaag agaacggaag actctttttc ctcggacatg atgatactta tattacactt
     9121 ttccgttgcg tacggacaag ggctgaactc cctttttcga atatgaaaac gctagcaacc
     9181 taccctaagg gctgtcaaac gaacggtttc cttggatttc cagcctcacc ggaacccgag
     9241 acccaggagc ttggaaatca gacgcgtgtg atttgattca caatagaaat tatcgataag
     9301 attggaaccg tgggtgagct tcaaaatttt cttcagtacc tcgagcgagg tgatgtcagc
     9361 gagatcgtgt tccagagcgg cgcagcagcc gcagccaaga agaagggaac actcaagccg
     9421 gtcacgcgcg agccgctgag cgcaaagcac attctcaagt tggtgagcaa cacacccctc
     9481 gaggtgctcg tgccggcaag cgacggcgcg accgaaccga cagcgatccg gatcgacgga
     9541 atcggttaca tcgcgaccat ggctcgctca ggcgagatgc tcatgctgcg tgtccgacgt
     9601 gaagggaagc acacgattcc gcctaaggtc ccgatcggcg cgatccaaat tgcgccggtg
     9661 cgcgtccccg atctgcagcc ggagacctcg cagccggagc tctcgcagcc agagattcgg
     9721 aagccggagc tctcgcggcc agaagtgcaa cgctcctacc gtccggcgcc ggaagcctct
     9781 cccgttgcgc ccttcgtgcc ggctggggtt gccttgcaag cgtcctccga gctccgcgcg
     9841 ctcctggccg cagcgcgcaa caagcctgcg agcgatgtgc acctcatgtc gggagagcct
     9901 gcacgatttc ggtcgagcgg ttatctcgtc cccgagggcc aggcactgag cgatcaggaa
     9961 gtcaccgccc tcgtgatgcc cttgctcgac gcgcgaaacg ccgcccagct cgatgagctg
    10021 ggatatgcgg atttcgcctg cgacgtcgaa ggcgcgggac gtcttcgtgt caatgtgagc
    10081 aagcagcgct ccggaatcaa gggttgcttt cgactgatca tgccggagcc gcccacgctg
    10141 gagtccctgg gcttgccacc cgagctccgt caaatgctcg actaccatca ggggctcgtc
    10201 gtgatctccg gtccgagtgg cgcgggaaag acgacgaccc ttgccgcgct ggtcgacctc
    10261 ttcaatgccg agcgcgcggt ccacatcatc acggtcgagg acccggtcga aatcatgcat
    10321 ccgaagaaga aggccatcgt gacgcagcgc gaggtcggag cccacaccca gagcttccac
    10381 actgcgttca aaggatcgct gcgcgaggac cccgatgtga tcgcaatcgg cgagctacga
    10441 gaccgtgaga ccgtcgagaa ggcgctcgaa gcggcggaga ccggtcactt ggtgttcgcc
    10501 acgatgagca caccatcagg cgcgagcacg atcgaccgtt tgatcgacat gtttcctccg
    10561 gacgaccagt cgcaggttcg tgcgaccctc gccggggcct tgaagttcgt ggtgagtcag
    10621 cggctcgttc cacgcaaaga tgggaagggg agagttgcgg ccgtggagct gctgaccggc
    10681 ggaatggcgc tttggagctt gatccgcgat gacaagctct tccaacttcc gagcctcctt
    10741 cagaggggac gcggctttgg tatgatccga atcgaggatt cgctgaacgc cttggtcgcc
    10801 accggcaccg tgacggtaga gacggccaag atcttcgccg atgacgcgcg actcatcggg
    10861 caagtccccg cacccgggag cggcgcgggc gcgccggaat cagcgtcgaa tagacgaaaa
    10921 ggaaacgtcc tcaacccttt cgcgaagaag gacagttaag cgatgcctgc tctcgaccag
    10981 tacttcgacg tgcttttgaa caagggagga tccgacctcc atctctcggt gggctatccg
    11041 cccaccatcc ggctgaaggg agagctcgtc ccaattgccg agcagccact cgatcgcctc
    11101 accatcaaga acctgatgga tgagcttctg gaccagaacc agcgcgatca cttccacgcg
    11161 taccacgacc tcgacttcgc gtacggctac gaagacaagg cccgcttccg ggccaactac
    11221 ctctacaaga cgacgggccc aggcgccgtg tttcgaacga ttccgagcga gattctgagc
    11281 gcgcaagacc tgggtctgcc cgccgccgtc gtcgagctcg caaatcggag atcaggcctc
    11341 gtcctggtca ccggtccgac gggcagcggc aagagcacca cgctggcggc catgatcgat
    11401 cacgtcaatc ggacccgaga agggcacatt ctcacgatcg aagatcccgt cgagttcgtg
    11461 caccggcccc gcaagtgtca gatcacgcat cgcgaggtcg gcatccacac accgagcttt
    11521 gcagacgcga ttcgcagcgc ggggcgcgag aacgccgacg tgattctagt gggcgagctt
    11581 cgcggcagag agacgatgaa aatggctctc cagctggcca gcttcggaat tctgatcttc
    11641 gcaacggtcc acacgaattc ggcgcctgcg acgatcgatc gattcgtcaa cgcgttcccc
    11701 gcgagccagc agccacagat tcgtggcatg ctcgccgatt cgctggcggg catcgtggcc
    11761 cagcaattgc tccgaaccgc ggatggaact ggccgagtcg ctgcgcagga gatcctcatc
    11821 ggaacgcccg gtgtcgcttc cgccattcga gaatcgaaga cctcgatgat ccaaagcctc
    11881 attcaggggg gcggcagcta cgggatgcag agcatggatg ccgtgctcgg ccggatggtc
    11941 gccgaccgca agatccgagc ggaggatgcc ctcgaaaagg cctcggacaa gatcagtttc
    12001 gtcaagctgc cgctggtcgc agcggcactg aagaccgcgc tcagttgacg gtgaacgaat
    12061 agcgcgcgcc catccgcggg gcaccgaacc ctgggaatcg cacagacttg atctgctgac
    12121 ccatgcatcc ctggaagcct gagctcccac cgtgaatcgt ggcgcccagc acggagccgt
    12181 cgcccgcgat cgcaaggtcg accttgacgc tgccgggctt accgccgctc ttcagctctg
    12241 ggatcacaca cctgctgtag aaccggttca ggttctgatt catgacgctg gcgacttgat
    12301 tgccggtcag ccggccctct ccaccgctct gggaggcgtc gccgatctcc atcgcttggc
    12361 tcatcgcctc ttcgtagctg tagaagcctc cgctccgcct agccggggct ggagccgttg
    12421 agccacgttt gcgctttcgg acgggcaggt cggggagcaa gccggcttcg ccagtgctga
    12481 tctttccgag cttgaagaga tcatcgagct cggtcgatgc ggcctcccgg atgtcgctcg
    12541 agcgtgacag caggaacccg cccaccgaga tgatggtgac gccgatcacg gcggcggcga
    12601 tcagccactt catcgccgag gatcgttttt ctgatctgtg cgcgttcacg cgcgccgctt
    12661 gttccgctgc gctcgtgtcc tggtgttcgc gctgcaggac gagctcgcgg aactccttcc
    12721 actcacgcaa gggtttgcgc tcgccagtgt cgatgttgaa agtgaggtcg tcgccggaga
    12781 agcctccgat gttgagccgt tcgatgagct cccgggcatt gaagggcccg tggtccatct
    12841 tgtcgcgcgc gaccatccag cgctgctttg ggtctgcgga tagctcgcca atcaggctgc
    12901 ggagaccacg tctgggcggc ggaggaggtg cgaggggagc ttgcgcatgg attgtgacgc
    12961 ccggaatctc gagatcagcc ggcggcagaa cggagccgag gtcgatgtcg agactcaggt
    13021 ctgcatccgc ggccggtggc acggagttgg cggcgctgac gaacggtagg agcgcattgc
    13081 gtaccgcctc tggcgattcg aagcgatcct tgggatctgg cgccaagcac tggaacagga
    13141 tgccgtcgag ctccgccggt atgtcgtccc gcacgtgtga aggcgcgacg aagttcgacc
    13201 caggggaccg cgtcgtcagg agctcgtaca ggatcgcccc gattccaaag acatcactcc
    13261 gcaacgtcgc gggctcaccg cgtttcacct cgggtgccag acatgcgtga tccgcgttct
    13321 tgaaaacatt ggggcctgcc gtgccgagca gcacttgacc tacgccaaaa tcgcggattt
    13381 tgacccgccc tccgcgggtg acccagacga tgctcggccg gagcgttccg tgggggccgt
    13441 gtccatgcgc gtaactgagg gcactacaga ggtgcgcgat gacgttgtac acgcccttca
    13501 gcgagagcgg ctcgcccttt tggtcgcgct ccttgatgaa ctcggaaaga cgggtcccgt
    13561 caatccattc tccagccacg aagtgctcgc ctcggggtgt acgcccaaca ccgaacactc
    13621 gggcaatgtt gcggtgggac aggttcgccg cgctgcgaca cgcttcgcgg aaatcaggcg
    13681 cagtctctac ggagaccagg tccggccgga tgacgtgcac gaggatggcg cgcttggtct
    13741 gctggtcttt ggcggaccag atcacagcct gcgggtcttc atcgatctga tcgatgacct
    13801 cgaatcttcc tccgaccaca gcgcccgggt gtaaccccga tggctccggc ctcgtcgacg
    13861 ctatcccctc aggcatcttc gaggatcaag atagacgacc cgggtagctc gtcccaaatt
    13921 tgggtatcag tctgccttgt ccagctcgag ctcgagctcc cgatcatgca ggatcgctcc
    13981 ggccatggac tcgatcacgt cctcgacccg gtcctcgggg acgtcgacca acgagtgccg
    14041 gtctcgaacc cgtatgcgcc cgatttcctt gcgcttcagg ccggttcgtt ctcgcatgag
    14101 gcgacccagc tcggaggcct tgagctcgtc gcgccggcct acgttcacac acaaggtcgc
    14161 agtagggcct tcgtcgtcgt cttcatcttc gtcttgggtg cgcctcttct tcttcttgcc
    14221 gccgttgctg tgctcgtcca catgctgctt gccccggcgt ctacgcgtcg ccgcgacctt
    14281 gtcgaggtct tccgactccg ttccaaagaa ggcggccagc aagcccgcga gcattcgctc
    14341 ggcgttctcg tgcgtcatga agcgtcgcac caaggcgagc tcgcgttccg tgggtgcgtt
    14401 ggggtaggcc acctcgatca gcgcgatgcg gtcggcctct tgccgcgtcc gttgttcgcc
    14461 ctcgctcggg agagtgcgct cgactggaaa gattcggtac tggatccgca gatagtacaa
    14521 aatcccaagc tcggtcggac cgaccaaggt gatcgccgtc cccgtgcgac ccgcgcgccc
    14581 ggttctgccg gtacggtgca cgtactgctc gacgttcgta ggaagcgaga agttgatgac
    14641 atgcgtcaga tgagagacgt cgatgccgcg agcagcaaca tcggtcgcca ccagaaatcg
    14701 cagctcttcg cggcgaacgc gcccgagcac ttcctcccgc tcgcgttgag gccgatcgcc
    14761 attcaaccac tcggcattga atcccgcacg ttgcaaggct gccgagactt cctcggtttc
    14821 ggccttggtg ttgcagaaaa tgagtgcgct ctctggatct tcgatctcta gaacggagat
    14881 gaggtttgcg acccgtccaa caccactcac catgtagacg aagtgctcta ttccgagcgc
    14941 gcccacgtgg tcgcccgaga gtccgaggaa ctgcgggtcg tgcatgtgct tttcggcgac
    15001 acgctggatc gcacggtcca ccgtggcgga aaacagcagc gtctggcgct ttttcgggag
    15061 ccgctcgatg atggcgtgca gctctttggc aaagcccatc gagagcatct cgtcggcttc
    15121 gtcgagcacc aacacccgga gccgcgacgc gtccatggtg cctcgcttga ggtggtcgag
    15181 aacacggcct ggcgttccgc tgatgacgcg ggcacccttc ttgagcgcct cgacctgctt
    15241 ttccatcgac gcgccgccgt acactgcgac ggtatcgacg ccgcgacgcg ccccgagctt
    15301 cgcgatctca cgcgcgcttt ggagcgcgag ctctcgcgta ggcgcgagga ccaatgcttg
    15361 ctcgtctcca tctctcgaca tcagacgatc gacgagcgga atcccaaatg cgcccgtttt
    15421 gccggtgccg gttcgggact gaacgatgat gtcgtgtcca gcgacagcga gcgggtaggt
    15481 gtcccgctgc acgggcgtgg gggtgtccca ccccatctct tcgatcgctt tgagcacatc
    15541 ctcgtgcaag ccaagctctg caaaagtctg gatgccttcc tcttcgggcg gctctgtcat
    15601 ggagtgggct ccggtggttc gggtgatggg taatccgctt tcgagccgag cgcttcgctt
    15661 acgcgattcg ccagaggtgc gtcttcacgc atgtacctct cgaggccgag ctcgacgcgg
    15721 tgtcgatatt gattgagaag atgcacctgg tcttcgtcgc atctcttcgc cagagcattg
    15781 agacgaagat cccaggattt gagtgcgcgt tcgagaggag ccacatcggt ttcgttggtg
    15841 gcgagataag cgaggcgcag ctcatcgact tcatggcgga gtagcctcag cccatcggcg
    15901 cactcgaggt cgaacgagtc gtcggctggc ggccagaaca ccgcgggaat taccgaagca
    15961 aagccgatca cgaccaagta ggagagggcg atcgcataga tgccaatcgc cagccggcgg
    16021 ccaagcccgc cccgttgttc ttttttcggc aaaaactttc ctagctcacc agacttctct
    16081 cgaaagcggc ttggttgtaa cgagccgccc ggacgcggtc aagacggcgg cggattgcgt
    16141 cgtttccgct tctcgcgatg ggttccccag aagaaaaggc cgacacccac gacgatggtc
    16201 agcgcaacga cgcctccgca caggccgtac cagaggaacg tagtgactcc ggttcccatg
    16261 cggtcagcag accagcttat acccgacgcc gtagacggtc aggatgtgga tgggccgatc
    16321 tggcttcggt tcgatcttgc gccgaagctt cacgatgaag ttgtcgacgg ttcgattgtt
    16381 cggccccgct tcgagccccc aaatcttctc cagaatttcg tcccgagaca cgggctcgcc
    16441 ctcccgctcc caaaggaact tcaagagctc cacctcgtag aaggacaaat gcatcgtgtc
    16501 cgatccgcga gtgagcgttt gtgaaccggt ttcgatgttg atgttgccga tccgaatctc
    16561 cgcttgggaa ggacggacgg agcgtccagc acgtcgaaag agtgctcgaa tgcgggcgac
    16621 cagctccccg atcgcaaatg gcttggtgac atagtcgtcg gcgccggctt cgaggcctcg
    16681 gatcttgtcc gactcctgtc cgcgcgccgt aagcatcagg atcggcagga tgggatcgcc
    16741 gcgacggatc gtctcgcaga cctggtatcc attcatgtcg ggcagcatca gatccaggat
    16801 cacgcagtcg acgtcctcag acgccgccag ctcgatggct ttcgcgcctt tggcggtgtg
    16861 cacgaccctg tagccttcga atcgaaggct gtcggacagg ccgagcgcca gcgccggatc
    16921 atcttcgacc agcaggattc cgcgtccgtc ttctgacatc gtggcgaaga tacccgtagt
    16981 accgccaagc ggcaaagccg cttcgctcag cccaaggcct tcccggccgc caccggaatg
    17041 gagaagccga ccacggcacc gccgccttgg gcgtttttcg cccaagcccg accaccatgg
    17101 gcttgcgcga tgtgtttgac cagcgacagc ccgatgcccg agccgcgagt ctgggtcgaa
    17161 gtcggtcccc ggtagaaccg ctcgaaaacc cgacgcagcg agtcgctggg aattcccggt
    17221 ccatggtcgc gcacagcgac gtcgaccatg ttccgtttcg acgtgacggt gagctccacc
    17281 ggagactgct ctccgtactt gacggcgttg tcgagcaaat tgatcactgc aagggcgatc
    17341 gcttcctggt ccatcagaac ctgcgggagg tcttctgatg cgttcagttg aatctcgact
    17401 ccatcttgct cgcttcggta tcgaaatgct tcgagagcgc gtccgatggc atcgccgagg
    17461 ttgccgcgct tcagacgata gggtcccttg cccccttcga gcgcggagaa atcgagcaca
    17521 ttgtcgatga gggaggtcag acgctcggac tccctgcaaa tgatgtcgag gtattcgagc
    17581 ctgcgagcgt cactgggtac acggcctgat cgaagcatgt ccgcaaacat tcgcacgacg
    17641 gacaacggtg tcttgagctc gtgagacacg ttggccatca gctcgctctt gagttgattc
    17701 agtcgccgtt ccttctcagc cgcgtagatg aagaagatcg cgctcagaag gatgatcgcg
    17761 aaggacatga tcggtagagt gagctcaccc tgcatgcgcg atcggcctcg tgcttgcaga
    17821 cgcgcggcct gccgcggcgc gacctggagg cgccaccgat agagcgtcct cgggaaacgg
    17881 tggtcgaccg tgtagactcc agcttcagag aggtcgggac caaaaatgat ctgattgctc
    17941 tcatcgacga tgttgtagag aggggtcgtt tcatctgaga cgaacatcgc ggggaagacc
    18001 tcgtcgacga gatacttcgt gtcgtgcacg gcgacgatga aggtgacttg gccggcgagc
    18061 gtggcgtaag cccgatagga gagcagatac ccctcgtcgt cgacctgctg gtgcaggtgc
    18121 cgcaggaggt tgggttccgc agcgtcgagc tcgagcgatt tcaatagatc ctggcggaaa
    18181 actcgcaaga aagccctgcg gtccttcttg tcctctcggc tcgcccattg aatgatgttt
    18241 ccatccgggt cgagcaagag caccgagcgg acactcggag tttgcgtggc ggcttgagga
    18301 agccacgccg tatcgatggt gctcgagtcg ttgggattca ccaaccgaaa cacggtgtgg
    18361 tccgcaacgg taatgtaacc ctcaatccgt tctacacgtt gctctgcgac ggaaagcaag
    18421 gactgggcga tcgtctgctc gccaagttgg gcgtagtctc tcgacgaacg aatcgtgtag
    18481 tacccgagga tcgcggcggc gacgaggatc gcgggaacca ggatgaaata cacgaggcga
    18541 aagcgaccgc cttccatcac ggacgccccg cgagccagtg gctattggct cgctcgaggc
    18601 tcccgatctc gattcctttg gtcgccgtca cgattcgagc ctgcgtgtcg cctcgtcaag
    18661 gagcggaggg ttgcacccct tcgcccttgt ccgggcgtgg atcgctgagg tcgagcttga
    18721 gtcgatccgg atcgagcagg gcattcgcta ggacggcgtc catcttggat acgggaacga
    18781 actctagggt ctcccgcacc tcctccggaa cctcgtcgag atccgcggtg ttgcgttctg
    18841 gcagaatgac gcgcttgatt cccgcccggt gtgcggcgag caccttctcc ttgatgccac
    18901 ccacgggaag cacacggccc cgcaaggtga tctcgccggt catcgcgacg tcatgacgca
    18961 cgtgaatgcc ggtgagcagc gagacgatgg ccgtcatcat agtgacgccc gcactcggtc
    19021 cgtctttggg catcgccccc gcgggaatgt gaatgtggat gtcgctcgtt tcgaggaaat
    19081 cctccgggat tccgaagctc ttggctcgcg tgcggacgta gctcatcgcg gtctgcgcgg
    19141 actccttcat gacgtcgcct agctgaccgg tgagttggag cttgcccgtg cccttcatct
    19201 tcgtgcactc gatgaacaag atctctccgc cgacggaggt ccaggcgagg cctgtggcca
    19261 ctcccgtttc cgcggtgcgt tccgcgacct ccgaactgaa ccggggtgca ccgaggtagg
    19321 gtttcagcga gtcctcgtcc gagatgatcc agggcccttc ctcgccttcg gcgaccttca
    19381 ctgcgacgcc gcgaatgacg ctcgcgattt gacgctctaa gttacgcact ccggcttcgc
    19441 gggtgtaatg gtcgatgatg gccgcgatcg ccccgtcttc caaggtgact tgctcgggct
    19501 tgatgccgtg ctcatcgagc tgctttggca ccaaatggac ccgcgcgatg tcggccttct
    19561 cgcggcgcgt gtagcccggg atctcgatga tctccatgcg gtcgcggagc ggtgccggga
    19621 tcgtgtcacc aacgttggcg gtcgcgatga acatcacctt cgaaagatcg taagggattt
    19681 cgagatagtg atcgctgaac gtgtcgttct gttctggatc gagcacctcg aggagtgccg
    19741 ctgcagggtc gccgcggaaa tcgtggccga tcttgtcgat ctcgtcgagc atgaagaccg
    19801 ggttgatcgt gctggctttc ttcatccctt ggatgacctg gccggggagg gcgcccacgt
    19861 aggtgcgtcg gtgaccgcga atcgcagcct cgtcatgcac accaccgaga ctgatgcggt
    19921 gaaacttccg tcccagcgcg cgcgcgaccg atcggccgag ggaggtcttg ccgacaccag
    19981 gtggcccgat gaggcagagg atcggaccct tcttgtcggt cttcagcttt cgcacggcca
    20041 gatactcgac gatgcgcttc ttgaccttct cgaggcccga gtgatcctcg tcgaggactt
    20101 tgcggacggc cgcgatgtcc atgttgtcgg ctgtctgctt gctccagggg atgtcgagga
    20161 tccagtcgat atacgtgcga acgacggtgt actcggccga gccgacttgc atctgcctga
    20221 gccgtttgag ttgcttgtgg gcgaccgtat cggcctcgcc tggcaggttc gccttcgcaa
    20281 tcctctcttc gaggatatcg aggtcgccct gatcgccttc ttcttcgccg agctcttcct
    20341 tgatggcctt gagctgctga cgcagcacgt actcgcgctg gttcttgccc atttcctctt
    20401 tgatctgcga gttgatccgc tcccgcatct tgaggatttc gagctgacgc gtgagtaagc
    20461 gcagaacctt ccggatgcgc tccttggcat cgagcgtctc cagaagttgg gacttctctt
    20521 cgaccggtgc atcgaggttg gccgcgacta aatcggccag ctgaccgtgc tcctgaatcg
    20581 agtcgattag cgagcccgcc tcgcggggca gctcgggcat gagctgaatg acctgcttgg
    20641 cgatgtcacg gaggcttagc gcgagcgctt cactctccac gtcctcgacg ctgggctcgt
    20701 ccaagcgaga cacacgcgcg cgcatatatg gctcggaggt gaccacttgc tcgaggcgaa
    20761 tgcgcaccag cccctgtaga atcagactgt agttgccgga gctgtgcttg agcgccttca
    20821 gaacgcgtgc cgcgacgccc acggggtaga gatcgctctg atcggggtcg tcggtcgagg
    20881 gatcgcgctg cgcgatgatt gcgatgatcg gctggtctag gttttcgatg tcctcgacga
    20941 gcgccacgga cttctctcga cccacgtcga agggggccac tgccgccgga aagaggaccg
    21001 cgttgcgaat cggtagaacc gggagctcgc taccaaactc gatctcgccc gagtcgggtg
    21061 ggggaatcga tgtgattgtg tcctcttcgt cgctcattcc ggtgttctac tccaactggg
    21121 tcagatccca agatttttcc agctactgtg ggcgaacata gccacgcacc ttttccgtgg
    21181 caagggtcag ctcggaacca ggcgctcgcc ggtccaggtc cgcagcctac aagccgattg
    21241 aagcccatgg gtcctcgcac taggctcggc ccgatgcgcc tttcggtggt ggttccgtgt
    21301 ttcaacgagg aagggaatat cgcgccggtg gtggcacagg ccgtctcagt gggacggagg
    21361 ctcgcctcgg agctcgaaat catcgtcgtg gacgatggca gcaccgatga gaccgctatg
    21421 gtcctcgaca ggctgcgaac gagcgtgccg gagctgcaga tcgtcgcgca cccacggaac
    21481 ctcggatacg gggcggcagt gcgttcggga ctcgatcgcg ctctgatgga ctacgtgttt
    21541 ctgacggacg gcgacggtca gttcgatctc gaggagttgc cgcaggcgat tcgattgctc
    21601 ggtgagcatg acgtcgtcgt cggataccgg accgcgcggc aagatggctg gtggcgccgc
    21661 ttgtggggcc ggtcctggac ggccttggtg aacttggtct tcggtctccg ggtaagggac
    21721 gcgaattgcg cattcaagct ggtcccccaa agccttctgc gcagcagcag gcttcggagc
    21781 cagggcgcgc tcatcagcgc cgagttgatt ttcgaagccc aacggtcgga ccttcgtgtg
    21841 ggcgagtgcg ccgtaagcca ctaccctcgc caagccggcc gccaaacggg tgcctcgctt
    21901 agggtcatcg cgaccgcgtt cttcgagctc gctgcgtgta tgccgcgaag atgggcaccc
    21961 gagaagcgcc tcgggccaca ccgaggttcg cgcctcgaac agagccggtt ccgttgagct
    22021 cgcgttagaa gcagacgcgg tttcgaccgg ttcgcttcgc ttcgtacatc ttttcgtccg
    22081 ccgctttgta caggtcggaa ggatcctcgt agtgcgtttg ccactccgcg actcctacgc
    22141 tcaccgtcac atcgacgcgc gtgccatcga agtcgaagcg ggtgtcgctg atcagtcggt
    22201 tgatcttccc cgcgagctct ttcgcgccat cgacaccgac ttcgggcgtg atcagggcga
    22261 attcctcgcc cccgattcga gccaatacgt cgttgacacg caacctcccg agaagcatgg
    22321 ccccgagctg tcgcagtatc gtgtctccgg ccaagtgacc aaagcgatcg ttgatcttct
    22381 tgaagtggtc gatgtcgaag accaaaagag agaagctgcg cttgtacctc ttcgcgcgcg
    22441 cgacctcgcg gtccagcatt tcgtcgaagt acctcttgtt gttgagttgg gtcagcccgt
    22501 ccgtcgtcat caagcgatag atctcttcgt ggtactccac ttccacgtcg tctccgacga
    22561 tgaacttcag aatcgtccgg ccgaccctga gttggtcgcc atggcggagg cgcccctcgt
    22621 ctccgacgag gttgtcgttg acataggttc cattggtcga gccgaggtcc cttacgacga
    22681 actcgccctc ttcgaatcca atccggcagt gattgcgcga gacgctctcc tggtcgatct
    22741 ggatctcgca cttgggcgag cgtccgatga tcacgggttc agtgctcacc cggactcggc
    22801 ggcccatctc cggtccgtag atgacgacga gacacccgtt ctcactccct cgaggggccg
    22861 tgcgggcttc accgacttgc gtgagcacgg tctggctggc gctcgtcgtc tggtccttga
    22921 acggcacctc cacaatatac agcggggagg ggtggtacgg taaatcagcg acaggcgatt
    22981 gcagtcacag cggaccgttg ggtttgttga cccacagaaa agggttcaat agcgtaggca
    23041 cgtgcgcgac ctattttctc actcctggct gtgggggctg gcgagcctcg ctctgattct
    23101 cggaacgccc ctggcgttga tggcgagccc gccggctgct caaagggctt cgacaaacgt
    23161 ggcgcgggtg ttgagccccc ccttcacgga tgtcgacatc gcgaaagcaa aacctggatc
    23221 cgggagctgg gtcgtcccgc gcagcgatgg cacgcgcgcc gttttgaccg tcgacggcga
    23281 gctccaatcc tacattcaaa ccctcttcag ccgctacgat gtgccggcgg gcggcctggt
    23341 cgcgatagaa ccgaagagcg ggcgagtgct cgcctacgtg ggctacgaag ccgggaaggg
    23401 caacagcccg caggtggtga ccgattcggg tccaccagcg gcatcggtgt tcaagatcat
    23461 caccgcctcg gccttgctcg acgccggcgt caagagctcc acgtcggttt gttacggcgg
    23521 cgggatgcgt ggtttggagc aagtggattt aatcgacaac ccgagtcgcg accgatggtg
    23581 cgcgacgatg gccgatgcgc tcggttactc gatcaatgcc gtgttcgcca agcttgccga
    23641 ccgtaacatc gatgaggctc gcatgaccca gtatgtctcg gccttcggct tcggtcagcg
    23701 tcttccgttc gaccttccga ccattccaag tcccgtagac gtgccagata gccgtttgga
    23761 acgggcgcga atggccgcgg gcttctggca cagtcagctc tcgccgttgc acggcgcact
    23821 tctggccgcg accatcgcga atgatggcaa gatgccctac gccgctctcg tcgagcggat
    23881 cgaaagtcaa accggcgaga cgctctacga acacaccccg cgcacttttc ggcaagtcgt
    23941 gcctcgccag accgcacgac tcgcaggcga aatgatgacc cgaacggtga cgaagggaac
    24001 ctcgaagaaa gccttctggg atccgaaagg tcgaccgttc ctccccggca ttgccgttgc
    24061 gggcaagact gggaccctca gtcgctacga gccccatcga acctacaact ggtgggtcgg
    24121 gtacgcgccg gtcgacgatc cgaaaatcgc cgttgcggct ctcgtggtca acgaaccgaa
    24181 gtggcgcatc aaggccagct acgtcgcgcg cgaggccttg cgccagtatc tgatgcgctg
    24241 aagacgtcga ggctcgcaag cccgccaaat cgtgtgttgc ggttgggtcg gaccctggct
    24301 aggctccccc gcgcttcact agaaagaaag gtcaacttca tgttccacag cattcgactc
    24361 ggggcactcg cggccctcac agccatctcg ttgctcggtg cgtccgctgc aaccgcgcag
    24421 tcaccagtgc tcagccgcgt catcaagacc gacacgcttc gagtcggaat gtcggcgaac
    24481 cagccgccgc tcaacatgaa gagtaagagc ggtcagatga tcggcctcga ggtcgacctc
    24541 gccaaggggc ttgcttacgc tctgggattc gagctcgaaa tcgtgcagaa gccgtttggc
    24601 gagcttctcg gcgctctcaa gaagggcaag gtcgacatgg tgatgtcggg cgtcgacatc
    24661 acagctgcgc gcagccaaga cttcctgttc gtcggtccct acctcttgtc ggggaagtct
    24721 ctggtcacga cctccaaggc gttggcaggc gcggagaccg tcgatgacat caacacgccg
    24781 aacgtgacct tcgtcacgct gaagaattcg accagccaag ccttcgtgaa gaagaacctt
    24841 ccgaaagcga agtcggtgac cgttgatgac tacgaggccg cggtggggat ggtcatcgat
    24901 gggaaggcaa gcgcgatgat cgcggaccta ccggcttgtg cgcttgcggt tctgcgaaac
    24961 cccgacgcag gcctcgtcac tctcgccgag cctctttccg tcgagcccat gggcatcgcg
    25021 gtgtcggcca aggatccgcg cttctacaat ctcatcgaga actacatcga cgcgatcgaa
    25081 gccaccggga ttctctcggg gttgcgtgag aagtggctcg aggatccgag ctggctcgac
    25141 gagctgccat aggtcgtcac cggggcgcgg ggaacgcgac acggggtttc atcggacggg
    25201 gcgccttgca aaggtggccg gaaggactgc gagcaggggc cacgcaaggc gcccctcgga
    25261 gctcgcgcca atcggagggg gctgctcgct gaccgattgt cactcacacg ccgccgaaac
    25321 cccgcatcgc attccccgcg cccctccata caccccccag tgccagtgcc agtgccagtg
    25381 ccagtgccag cgtcagtgcc agcgtcagtg cgggcgtgct tcgacgcgtg cggaggaggg
    25441 cggctcggga tgggcagcac agcgattggg tcgagcctag ccatagggcg tcttggggaa
    25501 ctgggggcga gtgggtcccg atcgcgagca cgcccgtcgc gagccgccct ccggggactc
    25561 cgtcgcgccc cggtcgatct agcccttgtt ccgaacgatg aaggtgttca tgccttcttc
    25621 gcgttcgaac gtcgttcggt agtcttgagc ctgctgtcgc gtttcgaagg gtccgatgcg
    25681 aactcgccag tgcatgcctc ggccctcgac cgaaccgctg gtcacgtacg cggcgtgtcc
    25741 gcgtttgcgt agcgcgctcg cgaagactcg ggcctcttcg gcgtccccat agctgatgac
    25801 ttgtaaggtg tagcgccctt cgtaccctgc aggtgacgac tgagttcttt cggtgacggt
    25861 ggccgcaacg agtgggtcgc ggatggcggc catctcgacg agcgtccggt cgggacccgc
    25921 ggtgacggcg gccggcaagc ctgccgcgat atcagatcgc ggggccgggc tggtcagagg
    25981 gtccagatgc gcgagctctg cagctgcggc ggccatggct gccgcaacct cggcgcgctc
    26041 ctcggtgacc aaggtggtcg gaaagctgag tgactcggga tcgatgtcgg ggtcctcggc
    26101 gcgctcgcct ccggcttcat gggcttccgt ctcgtccgct atggccagca tctgaagcag
    26161 gtcctcgggt cgcgcttcgt cgccgctcgg caacgcggcc accgcggcga ccacgattcc
    26221 gaccgcggcc acgaggccca cgcctgagag caccaagacc ccccgtccct cgtccgaaga
    26281 tcgctcttcg atccgttcta gatcgcgcat tgcagtgtcc atctttcgtt ccgcttcgaa
    26341 cctggtctcg ggtttacgaa acttccctcg aagcagccaa aaaacagcag ttcggaaggc
    26401 ctgagagcgc gtgcaggagg ggggcggtcg tggtagcttc gcggcggcgt cgcacatctg
    26461 gggaacgacg cgcgggagca gtaattcatg gcggaatctt cacagaagca tccggtcgcg
    26521 ctcgaagacg agatgcggcg ctcatacctc gactacgcga tgagcgtgat catcgggcgg
    26581 gcgattccgg acgtgaggga cggactcaaa ccggtccacc ggcggattct ctactcgatg
    26641 cacgagcaga aggttcattg gaacagctct tatcggaaga gtgctcgcat cgtcggtgat
    26701 gtcctcggca agtaccaccc tcacggggac acggcggtct acgacgcgct ggtccgcatg
    26761 gctcaggact tctccatgcg gtatccgctc gtcgacgggc agggcaactt cggctcggtc
    26821 gatggcgacc ctgccgccgc gatgcgctac accgaggtgc ggatgcacag gctcgcatcc
    26881 gagctcctcg cagatattga aaaggatacg gtcgacttcg ggccaaactt cgacgattcc
    26941 gaacgtgagc ccctggtcct gccgagcaga attccgaacc tcttgatcaa tggctcgggc
    27001 ggtatcgcgg tcggaatggc caccaatatc ccgccccaca atctgcgcga ggtggtcgac
    27061 gccacggtac gcctcatggg tgacccagag ctcacggtcg acgagctcat gcaggatgac
    27121 gacgaggcgg gccgccttgg cgtgaagggc ccagatttcc cgaccgcggg gtacatctat
    27181 ggccgcgcag gcattcacct tggctaccgg accggtcgcg ggcgaatcgt gatgcgtgcc
    27241 cgagccacga tcgaatcgct cccgggcaag ggcgatcgcg agatgatcgt gatcaccgag
    27301 atcccctacc aggtgaacaa ggccgagctt ctcaagaagg tcgccgattt ggtccgcgac
    27361 aagcggatcg agggcatcag cgacatccgg gacgaatcgg accgcgatgg cctacgggtc
    27421 gtcatcgagc tcaagcgcga cgcccatggc gaaatcgtgc tgaacaagct ctaccaaatg
    27481 acggcgcttc aaagcacctt tggctacaac tgcctcgcga tcgtgggcca gcggcccgag
    27541 gtcctcgacc tccggacagc gcttcttcgg ttcatcgacc accggcgcga ggtcgtcgtt
    27601 cgccgaaccc gctacgacct cgcccaggcc gaggcccagc gcgagctggt cgaaggtttg
    27661 gggatggcgg tcaccgacgt ggacctggtg gtgagcacga ttcgctcctc gaaggacccc
    27721 gacgaggcgc gcgagcgcct gatgaagctc ccgctcacgg ggctggagga gttcgtgaga
    27781 cgtgctggcc gccccgaatc agagatccaa gccgccaaag agcatggcga gtacctgctc
    27841 tccgagcgac aggccaaggc gattctcgag atgcgcttgt cgcgactcac cggcctcgaa
    27901 cgggagaagc tcgcgaccga gtacggcagc ctttgcgacg tgatcaagga gctccaggcg
    27961 atcctcgcaa gtaagaagct cttggacgaa gtcatcgtga cggagctcga cgagattcgc
    28021 gagcgcttcg gcgatgcgcg gcgcacggaa atcgtcgagg ccgagggaga gatttccatc
    28081 gaggatctcg ttccggacga ggaagtcgtc gtgaccgtgt cccatgcggg ctacgtcaaa
    28141 cgcgttccgc tgagcgagta tcggtcccaa gggcgtggtg gaaagggtct gcgggccatg
    28201 gacacccgtg acaaggattt cgtgagctgg gtgttcgtcg tgaatgccca tgcgaacgtt
    28261 cttttcctga gcgacaaggg caaggcctac ctcaagaagg tctatgagat tcccgagacc
    28321 agccgcgccg ctcggggtag agcggtggtg aatctcgttg gcatggagcc cgacgagcgt
    28381 gtggcggcga ttctgccaat tcgcgaattc gtcgaggatg gttatctatt gacctgcact
    28441 cgtcgtggcc gcgtcaagcg cacgagcctg ggtgcctacg aaaacattcg gcagactggg
    28501 atcatcgggg ttgcaatcgg tgaaggcgac gggcttctga ccgcgcgaat cgtgacgccc
    28561 ggtcaacacg tgctgatcgg cacatcgcat gggatgagca tccgctttcc catcgaagac
    28621 gttcgcgcca tgggccgcga ttcgatgggc gtgaagggca tcgacttgcg tgaaggcgac
    28681 ttcgtgatcg gcatggacat catcgaagat gaaggcgagc agcaggtatt gacggtgagc
    28741 gctaacggct atggcaaacg cacgaaggtc cccgagtggc ggatccagaa ccgtggaggc
    28801 aagggcatca tcgcgatgga cacctccgaa cgcaacggag cgctcgtgaa gctcaggctg
    28861 gtttcgcctg acgatcagct catggtgatc accaatggcg ggcaggtgat acgaacgcat
    28921 gtgggcgaga tccgtgagac gggccgcaac acccagggtg ttcgcgtgat tcgtctcggc
    28981 gaaggcgagc aggtcgtgga cgtggagccg gttgccgaag gcgaggacga tagcgccgag
    29041 gaatccccag aaaccgaaga aaattagccg atacgcactc ggaagtgtgt actccggtgc
    29101 acaagcggct attcttggaa agagatttgg gggagtcttt tctagggggt aggggagtgt
    29161 gagtaacgga cccgtcattg ggatcgacct cggtacgacg aattccgtgg tcgccgtcgc
    29221 cgaccagcct ggaggccgag ttctcgcgga gagcgatgga gcgaagctga ttccttcagt
    29281 cgtgtcgttc catccgagcg gtgacatctt ggtgggcgcc gccgcgaaag aacggcgcct
    29341 gctcgacgcg cagaacacgg tgtactcggt caagcggctc atcggccggc cttacaccag
    29401 ttacgaggtg aagcaggctc aggagcgttt cgcgttcgag ctctcggaag gcccgagtgg
    29461 aggcgtgctc gtcactgcac gcggcgagac gtatacgctg agcgagatca gcgcgttcgt
    29521 gcttcggcac gttcggaaag tggccgagga agcactgggg caggagtgca gccaagcggt
    29581 ggtgaccgtc ccggcgaact tcaacgagct tcagcgcagc gccacgaagg ctgctgggcg
    29641 ggtggcaggt ctcgacgtgc tgcgcattct caacgagcca accgccgcgg ctctggccta
    29701 cggctacaac aagacgtcga ccgagcgcgt tgcggtctac gatctcggcg gcggtacgtt
    29761 cgacatcacg atcctgcagc tcgcaggcga tgtgttcgag gtgctcgcca cggcgggcga
    29821 cacgttcctc ggcggcgacg acgtggacac gagcatcgcc gaccagatgg ctgcgcagtt
    29881 cctcgagcaa aatcgatacg acgcccgcca ggatcgtcag gcctacgagc gactccgcgc
    29941 agccgccgag tgggcgaaaa ttcagctttc ggacaagccc gacgtgaagc tctgcatcga
    30001 agagctcgcg tacggagagg gcggcgtcgc actcaatctg gactacgcgc tcacgcgtca
    30061 gcagct
//
LOCUS       DASRZF010000081        25046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_747858, whole genome shotgun sequence.
ACCESSION   DASRZF010000081 DASRZF010000000
VERSION     DASRZF010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 25046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25046
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747858"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..487)
                     /locus_tag="VFG22_03515"
     CDS             complement(80..487)
                     /locus_tag="VFG22_03515"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HET6415341.1"
                     /translation="MNELPPHVLFYDGVCAMCNGIVKRMLRLDRNGVFCFAPLQGATA
                     ERARALYAEFPEEIETIVYLRHGELFLRSRAAALAMGEMPYPAKALSWFRFLPAWLTD
                     FFYGIIARTRYRLFGKYDSCPVPPAEHRARFLP"
     gene            671..2938
                     /locus_tag="VFG22_03520"
     CDS             671..2938
                     /locus_tag="VFG22_03520"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET6415342.1"
                     /translation="MSGSWEKTACILCECNCGLEVQLGGEGQRHFTRIRGDKAHPASK
                     GYACEKPHRLDYYQNGPDRITSPLRRQEDGSFEEIDWDTAIREVAARLSAVRDTHGGA
                     SIFYYGGGGQGNHLPGAYSTATRRALGSRYRSSALAQEKTGEFWVGGRLMGSVTRADF
                     EHCEVGIFIGKNPWQSHGIPRARVTLKDIAKDPKRSLVVIDPRLTETAELADIHLRVK
                     PGRDAWLLAALAAVLVQEDRIDHGWIKAHVDGTEDVRAALGEIPVSRYCEIAGVSEAL
                     VRETARRIGDAESVAIFEDLGIQMNRHSTLNSYLEKLLWVLTGNFSKRGAQYVPASIV
                     PITGKLDKLAAGVESNRSPVAGERIISGLTPCNSIPDEILTDHPKRYRAMLVESGNPA
                     HSVADSQRMREALRSLDTLVVIDIAMTETARLADYILPAATQYEKAEATFFNFEFPEN
                     YFHVRQPLFDPPEGVLPEAEIHARLCEALGAYTEEDLAPLHAAAAQGRAAFIPVFFGT
                     VLSNPKLAPMAPVILYRTLGPHLPAGLQSAAVVWGLVHQFANANPGSLRRAGFHGNGF
                     EPAEKLFDAIVESPSGLIFAVDEQDESWARVKTPNGRIQAAIPELLEELASLGDGDLA
                     DAAFPFVLSAGERRSFTANTIFRDPEWRKKDAEGSLRINPGDASELGVDTGDRVRLTT
                     KRGSVDVSVELSQMMQPGHVSLPNGLGLDHTNEGGERVQTGVAPNELTATEDRDWLAG
                     TPWHKHVAARIEVIA"
     gene            complement(2940..3335)
                     /locus_tag="VFG22_03525"
     CDS             complement(2940..3335)
                     /locus_tag="VFG22_03525"
                     /inference="COORDINATES: protein motif:HMM:NF015581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HET6415343.1"
                     /translation="MNDPSYAFSKQLDGVSVDEALEKVTEALKANGFGVLTKIDVKET
                     LKKKIDVDFRPYVILGACNPKLAYKALQGESFIGLLLPCNVVVQESEGGALVSFADPK
                     AMFELVPDAPIAEVASEARRLLENARDQL"
     gene            3446..4525
                     /locus_tag="VFG22_03530"
     CDS             3446..4525
                     /locus_tag="VFG22_03530"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HET6415344.1"
                     /translation="MYKTLTAQTFHYFARPQERVLREPLTIPAAWKGSELALNDDWRE
                     ELTEADLVELDHAIATAKGTGKPMGDLSKEDFPLPGFSRKIDRWRREIQSGRGFQVIA
                     GVPVDRWTQPESELFFWCFGQHLGVPGAQNPDADLLGHVRDTGESPDEVRHYRTRVNI
                     NFHCDAADVVGLLCLRKAKRGGQSRIVSSISVYNELLRLRPDLVERLYEPFMMDTKGE
                     GGVQYLPVTPCRFDDGQLRTFYHTDYFRSARGFRGEPMLSAEDRALLDLYNEIADSAI
                     LYLDMDLEPGDVQLLSNHTVLHARTDYDDYPELERKRHLLRLWISLGARAPLASKLRL
                     HRSRAALATTVLRCKLGHALRRAIH"
     gene            4722..4856
                     /locus_tag="VFG22_03535"
     CDS             4722..4856
                     /locus_tag="VFG22_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415345.1"
                     /translation="MATSSARARDFTYLDEEKEPVYTSRWHEKNCAYMPWDYAAARAL
                     "
     gene            4884..5384
                     /locus_tag="VFG22_03540"
     CDS             4884..5384
                     /locus_tag="VFG22_03540"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET6415346.1"
                     /translation="MKRHLNSSLTVLVCLTLFVTMSIGCKKDTEEPEPEETTGSEIPE
                     PEPEVAVEPAEEPCVVETVYFAFDSSELDSSARASLQEAVECYRGQQDPGMSLLLTGA
                     CDPRGTEEYNIALGERRAQSVRSYLVSLGLDASRISITSVGEEMATGTDEAGWARDRN
                     VTGSEN"
     gene            5422..6141
                     /locus_tag="VFG22_03545"
     CDS             5422..6141
                     /locus_tag="VFG22_03545"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET6415347.1"
                     /translation="MLSVFLQASAPQQLNPWQLVVGASPVVQFVLVVLVFMMVMCLYI
                     IGAKWIRLKQVTDQSRGFLKVFWSDERTRTWNAKAMNDIHAQSASFERSPIATVFRAG
                     YRELARIARTGRPTKADVDNVERALRRAQSAELTRLENKVSFLATTASTAPFVGLFGT
                     VWGVMNSFIAIHGEKSAGLDVVAPGIAEALIATALGLAAAIPAVMGYNYFVRRIRVVD
                     SETSAFASDYLNIIRRHLLVE"
     gene            6144..6569
                     /locus_tag="VFG22_03550"
     CDS             6144..6569
                     /locus_tag="VFG22_03550"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HET6415348.1"
                     /translation="MAFSNDNGGRGTALSEINVTPLVDVMLVLLIIFMVAAPMLTTGV
                     NVELPKADAPRMDIDQEHPLITVQRDQRIFLFDEEVSLDVLRQRLVTDQRIRDVDEVF
                     VQADEQVPYGVVAQVLALVRQAGIGKMGLVTDPLTREPR"
     gene            6573..7391
                     /locus_tag="VFG22_03555"
     CDS             6573..7391
                     /locus_tag="VFG22_03555"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB C-terminal domain-containing protein"
                     /protein_id="HET6415349.1"
                     /translation="MVEASDWRALSLLETEQPTVAEIAGGIAFALAAFVLAPTLVLLS
                     AFVLGGVGSIFGLGIDELPPPTIEVIETRFVRLGKRKEQRRLPSKEVPEAQQTAPVPQ
                     SPDSEPMAAVPTGKTEKGLAKPKKEQKDRQQDLLSQLGERAGAISDLTKGPELEGDPE
                     GIVEGTKASGDETEIYIGKLYAYFRRGWQVPTLISDEELQKLACIIELSITDDGRVAG
                     YEFKRASGNEAFDESVRRRLSQAEGAELPKPPDSISNRILGETISLRFFGRHAR"
     gene            7400..8716
                     /locus_tag="VFG22_03560"
     CDS             7400..8716
                     /locus_tag="VFG22_03560"
                     /inference="COORDINATES: protein motif:HMM:NF015980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415350.1"
                     /translation="MTWNAIGNRWTRGVLLLGLVLAPALMSPPSAVGQEEAPPLPEDK
                     IVVDIDAPDEEVFRIGVPNFIGEPSAHAVNGSAILQNDFRLMPGFRVIGPSAVRHDYA
                     SKGLGVDLGAWALLGANGVIKGQVFGSEAALTVELRFYQSSGGEVPALSRRYHGPSSE
                     LRKWMHDFANAVIGVVTGVYGPFGTEIAFARKHGPGSKSVYAASMDGYGVREVSSGRG
                     VSMLPNFGAGAIWYTRMTAQGTFITNTRVGGKRLIGGTGITMAPAICGGRIYFSSSRS
                     GNYEIYSSNLEGKDVRRLTRSRAIDVSPTCGPSGKLAFVSDRHGSPQVFTMNINGTNV
                     QRVTFRGNHNQTPVWCSDPKQPLIAFSGRDTNFDIFTVNVETQEYTRLTQGQGDNKDP
                     AFSPDCRLIAFTSNRRGSEGVYLSGQNGLDQNQVITGAAETVRWAR"
     gene            8768..10984
                     /locus_tag="VFG22_03565"
     CDS             8768..10984
                     /locus_tag="VFG22_03565"
                     /inference="COORDINATES: protein motif:HMM:NF023649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HET6415351.1"
                     /translation="MATWKRGCLGMIGLTAVVALGLWVVLGGGKLQSDGEITAIPLSG
                     SALAARDRVQSAVADDAETRVLFGDLHVHSTLSVDAFQWSLPLMGGEGVHPPADACDF
                     ARFCSQLDFYALTDHAEALTPRTWDMVRESIRECNAVDAEAKQPDLVAFTGFEWTQIG
                     TTPEGHFGHRNVIFRETDDARLPTRPIAAPGLASQAFRDLKTIRSNLRIPFVTFPNQQ
                     PYLDASAHVREIARLSLCPEEGASPDLPVGCREIADTPEALFRKLDEWSFDAMVIPHG
                     TTWGFYTPPGYDYDKQIQAKHDNPKWQQLIEVFSGHGNSEEYRSFRAAERTEGGLVCP
                     KPSDSYEPCCWRAGEIVRGRCEDPLSEECDAHVKAARENFLRVGVSGQLTLPGTEATE
                     WGDCGQCTDCFQPSFEYRPGGSVQYVLARGSFDGSETPRHSTLGFIASSDNHAARPGT
                     GYKEFARRRMTEARGVTAPEWHELLFGNQEKTSESVPLSPEDVFARPPFRRVWLERQA
                     SFFLTGGLVAVHAKERSRDAIWQALFDREVYGTSGDRILLWFDLINAAGGRAPMGSQL
                     ELDGVPKFRVRAAGAFEQLPGCSKEVMEALGSERIDRICAGECYHPSDERRSITRIEV
                     VRISRQMSEAEAVEDLIEDPWLTIPCPSGQEVCEVEFEDPWYPSAGREVVYYVRAIQE
                     PTPAVNAGQLRCTGDECKPCYGDYRVSFDDDCLAMDQERAWSSPIYLTPPGTPAPE"
     gene            complement(10987..11811)
                     /locus_tag="VFG22_03570"
     CDS             complement(10987..11811)
                     /locus_tag="VFG22_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415352.1"
                     /translation="MGTFQQLRCTRPALVAGLIAVPLWLGAAPGCISQMRADIATNQE
                     RLDSLENDLEVKRKELEEALAEASRILRRNSADQGLQIEQLQQALAVIEGQVAELRME
                     SAGSSQAQAQRTLELQQRLNDVARAAGMDVAIDAEDIPKNKGEHWDALTKAYRINKYS
                     YTRALARAYVERYPKDENADNAQYMIGSSYLKQNQAAAALGEFQKVLSNYRKGDAIDE
                     TLYDMAQAFLQVRSCNDAGTALKALLNDHKSSKLAAEAKKELRKVRALGAADCDDR"
     gene            complement(11851..12504)
                     /locus_tag="VFG22_03575"
     CDS             complement(11851..12504)
                     /locus_tag="VFG22_03575"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6415353.1"
                     /translation="MHVGPGIGYLRPVTHGQLRNHYRIVLTGGPGGGKTTAADLFRRE
                     IGDKIVIVPETATMLFTGGFPRGGEPRARAATQQAIFHAQVALEDIQGALYPGRVQLC
                     DRGTIDGAAFWPESAPQGFFETLGTTLSDELLRYDAVIFFESAAVGDISIEGGNPART
                     ETNEEARQLDMRLRELWSKHPNFNFVPHSPSFFAKLQKGLGALQGIINENGLLHPHS"
     gene            12554..14173
                     /locus_tag="VFG22_03580"
     CDS             12554..14173
                     /locus_tag="VFG22_03580"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET6415354.1"
                     /translation="MSSEEHAPRTLLRGGCIVDGTGAAARSGSVLLEGGRIVAVGDLP
                     ADAGASIDCAGKVIAPGFIDAHSHMDFFSAGTDPHHFDSFAAQGVTTFVAGNCGFSPF
                     GFARNTKHRGLLENSLFKAGRESLDWNSFSEYRGVLDGLGLTQNFVHLVGHGAARTSL
                     SGFEARGLNASERAEMIALLDLALSEGAAGVSLGLQYKPGVFAKLDELEEVARLVKRH
                     DKLLTVHAKAYSSFSGTYPMVPFGRAHNLKAIDDMLGLARRTGVRLQFSHLIFVGSQT
                     WKTVDEAIELFDRAIADGVDVCFDMFPYACGATLLNTLLPEWFMARMPEALHSSIARA
                     RLRVEAEVGFRLVGFGYPEIQIINASCPEYDEHNGRFLPEIAERVGKSDFGTMLDILD
                     KSNAEARVLFHKYYNDEIIDRLMRHPAAIFASDSWPEPRGHQNPAAYGTFPRFLRKAR
                     ERGVVSLEEAVHKMTGAAADRFRLERRGCVREGCAADIVVFDWNLVRDGANRTGDAAP
                     SGVEHVFINGRPAIEAGAIHAVRGAGEVLLS"
     gene            complement(14221..15633)
                     /locus_tag="VFG22_03585"
     CDS             complement(14221..15633)
                     /locus_tag="VFG22_03585"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HET6415355.1"
                     /translation="MVPSPSFFSDVGQYFDAACQHTEVAPDILGQIRACNAVYRMRFP
                     VRRDDGRVVVVEAYRAEHSHHRLPTKGGIRYALDISQDEIMALSALMTYKCAVVDVPF
                     GGAKGGVCLDPRNESASFLERVTRRYTAELIRKRFIGPDVDVPAPDVGTGEREMGWIY
                     DTYKAYGPDTLNSLACVTGKAMSLHGVAGRREATGLGAIIALEYFLSQAEDVKPLGLE
                     VGLMGKRIIVTGLGKVGLHAALAAVQRDAVIVGVSVSDGALYDEGGLDVDAVLLHRAE
                     TGSLRGFQEARYLADPDAILQEPCDVLIPAALQHQITMRNASRLKAKVIVEAANGPID
                     PEADRFLRESGTVVLPDIYANAGGVVVSYFEWIKNLSHVSFERMTRRYQQISNNRLLG
                     AISRLTGKTLPPEEAALLLQAPNEIDFVRTALDNTMAIAYEKIREPWRRRALPDLRTS
                     AYLGAIESVANAYTLDGIFP"
     gene            15785..16996
                     /locus_tag="VFG22_03590"
     CDS             15785..16996
                     /locus_tag="VFG22_03590"
                     /inference="COORDINATES: protein motif:HMM:NF020932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HET6415356.1"
                     /translation="MTKMTKSWLVMGMLLGGCGGSADSEAGGPSTAALAQEATSTYAR
                     IAHQAYSDSLATAVMLTDALDAFVAEPSMESQEAAKAAWRAAREPYLQTEVLRFYDGP
                     IDNPTDGPEIYLNAWPLDEAYVDYTQDAASAGIINNRDIPIDEAALRGANEEGGEENI
                     SLGFHPIEFLLWGQDTTDPSDELPGLRSFTDYLIEEGGTAENQDRRGAYLSLLGNLLV
                     DDLEKLVNAWSPDDAGNYRAAFEGGGWEPALEKILTGMIILSGFEVAGQRLQVALDNG
                     DQEDEHSCFSDNTHRDMVQDVRGVQNVWLGEYQALDSTQSVSGTGLHEVVASIDGALA
                     AQITQQIAQSVSLAEALRPTEAAPPFDLLISLGNSEGNQKVQALIDSLRTQEDLLSSA
                     FVAFGLSVPEP"
     gene            17012..18400
                     /locus_tag="VFG22_03595"
     CDS             17012..18400
                     /locus_tag="VFG22_03595"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HET6415357.1"
                     /translation="MTRCTALGVVLAISLFGCSGGSTSPTADPAEAFPGGDTTTFFLF
                     AGNELAQPAANLEDRSPFFTGDSLFNQTWVTAPASTAARDGLGPTFNTRSCAGCHFRD
                     GRGRPPLQDGGTLESMLIRISIPGEDAHGGPLGDPSYGDQLQIDSLAGVPAEVKVRVY
                     WEEQQGAYEDGTPYTLRTPRYAYEDLAFGPLADDLRASGRTAPHMSGLGLLEAIPEET
                     LLDWADPEDADGDGISGRLNWVWDSVAQAMRPGRFGWKGEQPTVRQQVAGALLGDMGI
                     TSPLFLDQNCPAPQIECAAAPSGGEPEIGESNFSKLVFYSSTLAPPTRDDYDDPIVLR
                     GREWMRELGCTSCHKESAKTGEHDSIPELSSQKIRPYTDLLLHDMGEGLSDERPVFLA
                     EGREWRTPPLWGIGRVKVVNQHDFLLHDGRARGFAEAILWHGGEAEEAKDAFRSLPFE
                     ERESLIRFLESL"
     gene            18397..19503
                     /locus_tag="VFG22_03600"
     CDS             18397..19503
                     /locus_tag="VFG22_03600"
                     /inference="COORDINATES: protein motif:HMM:NF020932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HET6415358.1"
                     /translation="MNTPVRIVLLLAVVSAAVAGCFNEPFDQQAMLHSLGTDVIIPSY
                     ASFSEQTETLLVSARAFCEGATASRLGDLQARWKEARVHWKRTDVINFGPYMEQPWRL
                     GPKIDSWPVRETTVDENLAGEEPLNLESVSALGAASRGLPAMEYLIFDPAGSGVALAK
                     FEGAEGERRCDYVLALAEDLRGSAEAMVEAWSPEGDDYLGALLASGEPGAPFMSVRDA
                     SNEIVNRMVFLVENIMRLKLARPLGLEAGGDPQPNEVESRYSDHSIEDILANLDGLEN
                     IYRGRYGDRRGVGVQTWLRWGSPPIDTEMIAAIIRARSAVEAISEPLSRAVETEPALV
                     QTAYDQLRDLRNTIGVDVINALSGSATFNDTDSD"
     gene            19496..20851
                     /locus_tag="VFG22_03605"
     CDS             19496..20851
                     /locus_tag="VFG22_03605"
                     /inference="COORDINATES: protein motif:HMM:NF016946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HET6415359.1"
                     /translation="MTRPNLEKLRAPVDGASLGVFRAGFGLMMLVSVVRFWSKGWIDE
                     MYIQPALFFPYWGFEWVEPWSAEGMYLHFAILGALATLIMIGLWTRPALLLFFFGFTY
                     VELIDRTTYLNHYYLISVVSLLMVFLPVERSFSLSRALGRTTGPETVPRWSVYTLRMQ
                     VGLVYVTAGLAKLRADWLFRAEPLRTWLSWNADIPVVGPLLAEPWVGFAMSWAGAFFD
                     LFIVAFLLWRPTRVYAFVAVVLFHAFTGALFPIGVFPVVMALSATIFFEPDWPRRLIG
                     PPSAVSQTPPPALAPLGRWQKLGFALLTAHFVFQVAFPWRQLLYPGNACWTEQGFRLS
                     WHVMLIEKTGQVDFRIVDEPSGKTWLVFPRERLSAFQHRMMSTQPDMILSFAHHLRDE
                     WKAEGYPNVAVYADAWASLNGRRRQRLVDPAVDLAREEESWANKTWIVPLEDTVEARR
                     E"
     gene            21000..21782
                     /locus_tag="VFG22_03610"
     CDS             21000..21782
                     /locus_tag="VFG22_03610"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6415360.1"
                     /translation="MLFIMGFGMPGAVWSPQVDELQSDHRCCHYDHLGVGASEPGPLL
                     RTIPAMADDAVRILDDLSWDRAHVVGVSMGGMIAQELALRSADRCQTLTLIATHGGAP
                     IASLPTLRGMGLFLKGLFGGPKARMQSLPRLLYPPEFLETIGADGLRAHLKVRLGQPA
                     RLRTVLGQLFAVWRHSTEARVSQIDIPTLLVRPGKDILIRPTQTDRLAKRIPNARVLR
                     FDDAGHGVTFQKASELNAALRTHFAEDGNRRPKASSAPRARA"
     gene            complement(21838..24267)
                     /locus_tag="VFG22_03615"
     CDS             complement(21838..24267)
                     /locus_tag="VFG22_03615"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6415361.1"
                     /translation="MALEDARPDRFDGASAAEHEPVKALDRKLLREVVRLRGQALTIA
                     IVVASGVACYIALQSAWRALYQSRDLYYQTNHFAEVFVHLERAPKSVAGQVSELPGVA
                     RAYPRIVEQVRLPMDDLISPARGDLISVPSTGRAPMNGLVLRAGRNLEPGRYDEVLLN
                     EPFAKAHGLEPGDHISAVINGKLRDLLVVGHALSPEYVFAVDSGLLMPDDERFTPLWM
                     LEDAIAAAYRMEGAFNDLSVKLEPGANQDSVLSAIDRVLKPYGGLGAYTREHQLSHSV
                     LTSEIQGIETLVIFLPTVFLGVAAFLLNVVFSRLVALQRPQIAVLKAMGYRNATIATH
                     YLKMVALIVSFGALIGLGLGVWLGKGMLAMYAEFFRLPQMPQRVDLSVSINAILFSLI
                     AGALGASMSVRAAVKLPPAAAMRPPAPAKYRTAALERWGMTKWMSQPALMVFREIWRS
                     PGRTALSSLGIALALSIVILGRFGYDAYGPMMMVQFERQQREDLTVSFSDAMPERGLG
                     YLAHVPGVEEVEGIRATPVRMRSKHHWRDTMLMGIPDASRLRQLFNLEFKNVPIPKDG
                     VLLTSVLGKILNVEPGDVVQIELKEGDRGTRELTVAGLIEEGFGLQGYLSKDNLHRLL
                     RESPRFSTALLRIDVPTEAEVQSRLNEIPAIQAVSRKSDAIASFEKQTDETMKVMTLI
                     MTLFATAIAISIVYNNARVSVSMRGRDLATLRVLGFTRGEISAILLGEQAVQIAIAIP
                     LGLLLGTLGAKMLIASWADPEIFRIPFIISDQTYAFSVAVVLASGIASALLVRRKLDR
                     LDLIAVLKTRE"
     gene            24350..>25046
                     /locus_tag="VFG22_03620"
     CDS             24350..>25046
                     /locus_tag="VFG22_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415362.1"
                     /translation="MSLLLGCNSMGSGDSAPVQLGVVRTVDEARLEDPGNWGDELDLA
                     LETEFWDLIGNSDHARYPAWHSKAKAYVAANFDADPRLGMLISAGAVFSLTTLFQYDD
                     PLAHLAEYSDYVADATVGIHDAIEPLGDHEPALVFFHNNQAMLAFLLSENADLGICHL
                     EKLRNLEDSNPTFHGTEGRAAAPFTYGMVDDPAYVDYGIAMMEGCNNWICNWTSKLAP
                     FKPIGQLMTLAELY"
BASE COUNT         4784 a         7625 c         7769 g         4868 t
ORIGIN      
        1 cctcggatca ggttagcaat ccgtgccgct tcacccaaac gggtgggaca tttgcttaca
       61 tcgagttgcg ccggacgact caaggaagaa aacgcgcccg atgctccgcg gggggcactg
      121 ggcagctatc gtatttgccg aacaggcgat atctcgtcct cgctatgatc ccgtagaaga
      181 aatcggtgag ccaggcgggg agaaagcgaa accaagagag ggccttggcc gggtaaggca
      241 tctcgcccat cgccagggct gcagcccgcg atcgcagaaa cagctcgccg tgcctcaggt
      301 agacgattgt ttcgatctct tccgggaact cggcgtagag cgcgcgggcg cgttcggcgg
      361 tcgcaccctg cagtggagca aagcaaaaca ccccgtttcg gtcgagacgg agcatgcgtt
      421 tcacgatgcc attacacatc gcgcagactc catcgtagaa gaggacatgc ggaggcagct
      481 cgttcatcgg agctctcata gcagacgccc agcttcgagc ggccccggcc actttcgtag
      541 atatgaccgt gcgacctgag cgcttgcagg ctccgcggac gaatcgcagg cgcgctcagg
      601 gttacgcggg agtctgacta ccctaggatg gcgtggtcag cattggatgg cggaagagcg
      661 aggttgcagc atgtccgggt cctgggagaa aacagcctgc attctctgcg agtgcaattg
      721 cggtctcgaa gtgcagctgg ggggcgaggg ccagcgccat ttcactcgaa tccgagggga
      781 caaagcgcac cccgcatcaa agggatacgc ctgcgagaag ccccatcgcc tcgactacta
      841 tcaaaacgga cccgaccgaa tcacctcacc gctccgtcgc caggaagacg gcagcttcga
      901 ggagatcgat tgggacacgg cgatccgaga ggtggcggcc cgcctttccg ccgtgcggga
      961 cacacacggt ggcgcctcga ttttctacta cggcgggggc ggacaaggta accaccttcc
     1021 cggcgcgtac tcgacggcga cccggcgtgc gctcgggtct cgttatcgtt cgagcgcctt
     1081 ggcgcaggag aagaccggcg agttctgggt cggcgggcga ctcatgggga gcgtgacgcg
     1141 tgcggacttc gagcattgcg aagtcggcat cttcatcggc aagaacccct ggcagtcgca
     1201 cggcatcccc cgcgcgcgcg tgacgctcaa ggacatcgcg aaggacccca aacgcagtct
     1261 ggtggtcatc gacccgcgcc tcaccgaaac ggccgagctc gcggacatcc atctccgcgt
     1321 gaagccgggt agggacgcgt ggctgctcgc ggcgcttgcc gcagtcctcg ttcaagaaga
     1381 ccgcatcgac cacggctgga tcaaagcgca cgtcgatggc accgaagacg tgcgggccgc
     1441 cttaggcgag attcccgtct cccggtattg cgagatcgct ggcgtctcag aagcgctcgt
     1501 tcgcgaaacg gcccgccgaa tcggggacgc cgagagcgtg gcgattttcg aggacctcgg
     1561 cattcaaatg aaccgacact cgacgctcaa cagctacctc gagaagctcc tgtgggtgct
     1621 caccgggaac ttctccaaac ggggagcgca gtacgttccg gcgtcgatcg tacccatcac
     1681 ggggaagctc gataagctcg ccgctggcgt cgaaagcaat cgcagtcccg tcgctggtga
     1741 gcgaatcatc tccgggctca ctccttgcaa ctcgataccg gacgaaattc tcacagatca
     1801 tccgaagcgg taccgggcca tgctcgtcga aagtggaaac ccggcgcact ccgttgccga
     1861 cagccagcgg atgcgggagg cgctgcgctc tctcgacacg ctcgtggtca tcgacatcgc
     1921 gatgaccgag accgctcgcc tcgcagacta catccttcct gccgcgacgc agtacgaaaa
     1981 agcagaggcg acattcttca acttcgagtt tccggagaac tatttccatg tccggcagcc
     2041 cctcttcgat ccgcccgagg gggttctccc agaagcggag atccatgcgc gcctctgcga
     2101 agcgctgggc gcgtacaccg aggaagatct cgcgcctttg catgctgccg ctgcgcaggg
     2161 ccgcgccgcg ttcattcccg tgttcttcgg aacggtcttg agcaatccaa agctcgctcc
     2221 catggcaccc gtcattctct accgaacgct tggaccgcat cttccggctg gccttcagtc
     2281 cgcagcggtg gtctggggtt tggtgcatca gttcgcgaac gccaacccgg gctcgcttcg
     2341 tcgcgcaggc ttccacggca atggcttcga acccgccgag aagctcttcg atgccatcgt
     2401 cgagagccct tcgggattga tttttgccgt cgacgagcaa gacgagagct gggctcgggt
     2461 caagactccg aatggcagaa ttcaggctgc aatcccggag ctgctcgagg aactggccag
     2521 cttgggcgat ggggacctgg cagacgccgc gtttccgttc gtcttgtctg ccggcgaaag
     2581 acgctcgttc acggccaata cgatcttccg cgatccggag tggcggaaga aggacgccga
     2641 gggctcactc cgcatcaatc ccggcgacgc ctcggagctc ggggtcgaca ccggtgaccg
     2701 ggttcgactg acgaccaagc gcggctcggt cgacgtcagc gtcgagctct cccaaatgat
     2761 gcagccggga cacgtttcgc taccgaacgg cctcggtctc gatcacacca atgagggcgg
     2821 cgaacgagtt caaaccggcg tagcgccgaa cgagctaacc gccacggagg accgggattg
     2881 gcttgccggc accccttggc acaagcacgt cgcagcgcgc atcgaggtca tcgcctagct
     2941 cagagctggt cccgcgcgtt ctcgagaagc ctccgcgcct cgcttgccac ctccgcgatc
     3001 ggtgcgtccg gaacgagctc gaacatcgct ttggggtctg cgaacgagac cagggcaccg
     3061 ccctcggact cctgcacgac gacgttgcaa ggcaacagca atccgatgaa agactctcct
     3121 tgcagagcct tgtaggccag cttggggttg caggcgccga ggattacgta cggtcggaaa
     3181 tcgacatcga tcttcttttt cagggtttcc ttcacgtcga ttttagtcag cacgccgaac
     3241 ccgtttgcct tgagagcttc agtcaccttc tcgagcgctt catccaccga gactccgtcg
     3301 agttgtttcg aaaacgcata gcttgggtcg ttcatcgctt gcctcctcgg acacctataa
     3361 gaccgccttg tgaacgagcc aagcgtgccg caaaatatgc gtctcgggtc ttcgcgcaga
     3421 ggtgtaggca cccggaacga gcagcgtgta caagactctc acagcccaaa cgtttcacta
     3481 cttcgcacgg ccgcaagaac gcgtgctcag ggaaccgctg acgatcccag cagcatggaa
     3541 aggcagcgag ctcgccctga acgatgactg gcgagaagag ctcaccgagg cggacctcgt
     3601 cgagctcgat catgcgatcg ccaccgccaa gggaaccggc aagccaatgg gagacctcag
     3661 caaagaggat tttccattgc ctggcttctc gcggaaaatc gaccgatggc gacgagagat
     3721 ccagtcgggc cgcggcttcc aggtcattgc gggcgtgccc gtcgatcggt ggacgcagcc
     3781 cgagtcggag ctgttcttct ggtgcttcgg gcagcacctc ggcgttccag gcgcccaaaa
     3841 ccccgatgcc gacctgcttg gacacgttcg cgacaccggc gagagtccgg acgaggtgcg
     3901 ccactaccgc actcgcgtga acatcaactt ccactgcgac gctgcagacg tggtgggact
     3961 cctctgtctt cgcaaggcca agcgcggcgg gcagagtcga atcgtcagtt cgatttcggt
     4021 ctacaacgag ctcctgcgcc tgcgcccgga tctggtcgag cgcttgtacg agccgttcat
     4081 gatggacacg aagggcgagg gcggcgtgca gtaccttcct gtgacgcctt gtcgtttcga
     4141 cgacggccag ttgcggacgt tctatcacac cgactatttt cggtcggcac gcggtttccg
     4201 cggagagccg atgctctcgg ccgaggaccg agccctgctc gacctttaca atgagatagc
     4261 cgattctgcg atcctctacc tcgacatgga cctggaaccg ggcgacgtgc agctgctctc
     4321 caaccacacg gtcctgcatg cgaggacgga ctacgacgac tatcccgagc tcgagcggaa
     4381 gcggcacctc ttgagactat ggatctcgct tggagctcga gcgcctcttg cgtcgaagct
     4441 ccggcttcac cgaagtcgtg cggcgctcgc gacgaccgtg ctgaggtgca agctggggca
     4501 cgcgctccgg agggctatcc actgagctcg aggcacgctt gaaccgccgc cgccgggctg
     4561 ttccgtctct ggcgtcgtgc tctgtgtatc gagtttgaac ccggcctgtc gacctagcgc
     4621 gcgacgatat tccgttgcca gccgcgccga ggactgtcac aagcgctgtt gaaggaaggg
     4681 cggtccagat acgaagacgg cccagtgcta taaatttaca attggcgaca tcttcggccc
     4741 gggcacgcga cttcacgtat ttagacgagg aaaaagagcc cgtgtacacc tcgcgctggc
     4801 acgagaagaa ttgcgcgtac atgccctggg actatgctgc cgctcgcgct ctctgagttc
     4861 agaggggaag gaaaaggaac tccatgaaga gacatctcaa ctcatctctc acagtgctgg
     4921 tgtgcctcac tttgtttgtg accatgtcga ttggttgcaa gaaggatact gaggagccgg
     4981 aacccgaaga gaccactggc tcggagattc ccgagccgga acccgaagtc gccgtcgagc
     5041 cagccgagga accctgtgtc gtcgaaaccg tgtacttcgc gttcgactcc tcagagctcg
     5101 acagcagcgc acgcgcttcg ctccaggagg cagtcgagtg ctaccgcggt cagcaagatc
     5161 cgggcatgtc gcttctcttg acgggagcgt gtgatcctcg cggaacggag gaatacaaca
     5221 ttgcgcttgg tgagcgccga gctcagtccg ttcgaagcta cctggtttcg ctcggtttgg
     5281 atgcaagccg catttcgatc acctctgttg gtgaagagat ggcgacgggc acggacgagg
     5341 ccggctgggc ccgcgaccgt aacgtcacgg gttctgagaa ctgatcggtc gaggggggcg
     5401 ctcgttgggg gttagccatt gatgctttcg gtattccttc aagcgagcgc accgcaacaa
     5461 ctgaatccct ggcaactcgt ggtcggcgct tcgccggtcg tgcagttcgt cttagtcgtc
     5521 ctcgtcttca tgatggtgat gtgtttgtac atcatcgggg cgaagtggat tcgcttgaag
     5581 caggtcactg accagagccg gggtttcctc aaggtgttct ggtcggacga gcggacgcgc
     5641 acttggaacg ccaaggccat gaacgacatt cacgctcagt ctgcgagctt cgagcgttct
     5701 ccgatcgcaa cggtctttcg tgcggggtat cgcgagttgg cgcgcatcgc gcgcaccggg
     5761 cggcccacca aggcggacgt ggacaacgtc gagcgggccc tccggcgggc tcagagcgcc
     5821 gagctgacgc gtctcgaaaa caaggtgtcg tttctggcga ccacggcttc gactgcaccg
     5881 ttcgtcggac tcttcggtac ggtctggggt gtgatgaact cgttcatcgc catccacgga
     5941 gaaaagagcg ccggcctcga cgtcgttgct cctgggatcg ccgaagcatt gattgcgacg
     6001 gcacttggac ttgccgccgc gatccccgcg gtgatgggtt acaactattt cgttcgccgg
     6061 atacgggtcg tcgatagcga gacctcggca ttcgcgagcg actatttgaa catcatccgg
     6121 cgtcacctgt tggtggagta ggcatggcgt tctcgaacga caacggagga cggggtaccg
     6181 cgctcagcga gatcaacgtc acgccgctgg tcgacgtgat gctggttctg ctgatcatct
     6241 tcatggtcgc tgcgccgatg ctcacgactg gggtcaacgt cgagcttcca aaggcggacg
     6301 cgccgcgcat ggacatcgat caagagcacc cgttgatcac cgtccagcgc gaccaacgga
     6361 tctttctctt cgacgaagaa gtgtcgctcg atgtgctgcg gcagcggctc gtgaccgacc
     6421 agcggattcg tgatgtcgac gaggtgttcg tgcaggccga cgagcaagtg ccgtacggcg
     6481 tcgtcgctca agtgctcgcg ctggtgcgtc aggctggcat cggcaagatg gggctggtga
     6541 ccgacccgct gacccgagag ccgagatagc cgatggtgga agcgagcgac tggcgggcgc
     6601 tttctcttct ggagacggag cagccgacgg tggcggagat cgcgggaggc atcgcttttg
     6661 cattggccgc gttcgtcttg gctccgactc tagtgttgtt gagtgcgttc gtgctcggag
     6721 gcgtgggttc gatctttggt ctgggcatcg atgagcttcc acccccaacg atcgaggtga
     6781 tcgagacacg attcgtacgg ctcgggaagc gcaaggagca acgaagattg ccgagcaaag
     6841 aggttcctga ggcgcagcag acggcgcccg tgcctcagtc tccagattcg gagcccatgg
     6901 cggcggttcc caccggaaag acggaaaaag ggttagctaa gccaaagaaa gagcagaagg
     6961 accgtcaaca ggacttgctg tcgcagctgg gtgaacgcgc gggtgcgatt tccgatctga
     7021 ccaaaggtcc ggagctcgaa ggtgacccgg aaggtattgt cgaagggacc aaagccagcg
     7081 gggacgagac ggagatctac atcggcaagc tctacgcgta ctttcgccgg ggctggcagg
     7141 taccgacgct gatcagcgat gaggagcttc agaagctagc ctgcatcatc gagctctcga
     7201 tcaccgacga cgggcgtgtg gccggatacg agttcaagcg ggccagcgga aatgaagcat
     7261 tcgacgagtc cgttcgtcgg agattgagcc aagcagaggg ggcggagctt cccaagccgc
     7321 cagattcgat ctcgaatcga atcctggggg agacgatctc tttgcgtttc ttcggacggc
     7381 atgcacgttg aggttacaca tgacttggaa tgcaattgga aatcgatgga ctcgaggtgt
     7441 tttactgctg ggactcgtcc ttgcgccggc cctgatgtcg cctccctctg cagtcgggca
     7501 ggaagaggcg cccccgctgc cagaggacaa gatcgtcgtc gacatcgacg ctcccgatga
     7561 ggaggtgttc cggatcggcg tgccgaattt catcggggag ccctctgcgc atgcggtcaa
     7621 cggcagcgcc atactgcaaa acgactttcg cctgatgccc ggcttcagag tgatcggtcc
     7681 gagcgcggtt cgccacgact acgccagcaa ggggctgggt gttgatctgg gggcttgggc
     7741 cctgctcggt gcaaatggcg tcatcaaggg ccaggtcttc gggagcgaag ccgcgctgac
     7801 cgtcgagctc cgcttctatc agtcgagtgg aggcgaggtg cccgcgctca gccgaaggta
     7861 tcacgggcct tcgagcgagc ttcggaagtg gatgcatgac tttgccaatg cagtgatcgg
     7921 agtggtgacc ggagtctacg gaccgttcgg caccgagatc gcgtttgcgc gcaagcacgg
     7981 gcccggaagc aagagcgtgt acgccgcctc gatggatgga tatggcgttc gcgaggtgtc
     8041 gagcggccga ggcgtgtcga tgcttcccaa tttcggggcc ggagccatct ggtacactcg
     8101 gatgactgct caaggcacct tcatcaccaa cacgcgcgtt ggcggcaaac ggctcatcgg
     8161 tggaacaggg atcacgatgg cgccggcgat ctgcggaggc cgtatttatt tctcgtcctc
     8221 gaggtccggg aattacgaaa tctacagctc gaacctcgaa ggaaaagacg tgcgacgtct
     8281 cacacgtagt cgcgcgatcg acgtgagccc tacttgcggt ccaagtggaa agcttgcgtt
     8341 cgtttcggat cgccacggaa gcccacaggt gttcacgatg aacatcaacg ggacgaacgt
     8401 gcagcgagtc acgttccgcg ggaatcacaa tcagactccc gtctggtgca gtgaccccaa
     8461 gcagcccctg atcgccttca gcggacgcga cacgaatttc gacatcttca cggtgaacgt
     8521 cgagacgcag gagtacacgc ggctcaccca agggcagggt gacaacaaag atccggcgtt
     8581 ctctccagac tgccgcttga tcgcgtttac atcgaaccgg cgaggctccg aaggcgtcta
     8641 tctctcagga cagaacggac tcgatcagaa ccaggtgatc acgggcgcgg ccgagacggt
     8701 ccgctgggcg cgctgagcga acgaatctgc tttgctgcag agggccgcgc ctctatgctc
     8761 ggccaccatg gcgacgtgga agcgaggctg cctcggaatg attgggctga ccgccgtcgt
     8821 cgcgctcggc ctctgggtag tgctaggcgg cggcaagctc caatccgatg gcgagatcac
     8881 ggcgatcccg ctcagcggaa gcgcgttggc cgcgcgagac cgcgtgcaga gcgcggtcgc
     8941 agacgacgcg gagacgcgtg tgctcttcgg tgaccttcac gtgcattcga ccctttcggt
     9001 agacgcgttt cagtggagtc ttcccttgat ggggggcgaa ggggttcatc cacctgcgga
     9061 cgcctgcgat ttcgctcggt tctgctcgca gctcgacttc tacgcgctga ccgaccatgc
     9121 cgaggcgctc acgccccgca cctgggacat ggtccgagag tcgatacgcg agtgcaacgc
     9181 ggtcgatgcg gaagcaaagc aaccagatct cgtcgcattc actgggttcg aatggacaca
     9241 gattggaacg acgccggaag gtcacttcgg ccaccgcaac gtcatcttca gagaaaccga
     9301 cgatgcgcga ttgccgactc ggccgatcgc ggcgccgggg ctggcctcac aggcttttcg
     9361 ggatctgaag accattcgct cgaacctcag gattccattc gtcacgtttc caaaccagca
     9421 gccgtatcta gacgcatcgg cgcacgtccg agagatcgcc cgtctttcgc tatgccccga
     9481 ggagggtgca tcacccgacc tacccgtggg ctgtcgagag atcgcagaca ctcccgaagc
     9541 gctgttccgg aagctcgacg aatggtcctt cgacgccatg gtgattcccc atggcaccac
     9601 ctggggtttc tacacaccgc ccgggtacga ctacgacaag cagattcagg ccaagcatga
     9661 caacccaaag tggcagcagc tgatcgaggt gttctcgggg cacggcaatt cagaggagta
     9721 ccgcagcttt cgtgcggctg agcgaaccga gggcggcctc gtctgtccca agcccagcga
     9781 cagttacgag ccatgctgct ggcgcgccgg ggagatcgtc cgcggtcgat gcgaggaccc
     9841 gttgtccgag gaatgcgacg ctcacgtgaa agcggcgcgg gaaaacttct tgcgtgtggg
     9901 cgtgtcgggc cagctcacgc taccgggaac cgaggccacc gaatggggcg actgtggcca
     9961 gtgtaccgat tgctttcagc cgagcttcga gtaccggcct ggcggatcgg ttcaatatgt
    10021 cctcgccaga ggcagcttcg acggctcgga gacgcctcgg cattcgacgc ttggcttcat
    10081 cgcatcgagc gacaatcacg cggctcgtcc ggggaccgga tacaaggagt tcgcccgcag
    10141 gaggatgacc gaggcccgag gcgtcactgc acctgaatgg cacgagcttc tgttcggaaa
    10201 ccaagagaag acgagcgagt cggtcccgct gagccccgaa gacgtgttcg cccgcccgcc
    10261 atttcgtcgc gtatggttgg aacgtcaggc ctcgttcttt ctgaccggtg gcctcgtcgc
    10321 cgtgcacgca aaggaacggt ctcgagatgc catctggcag gctctgttcg atcgagaggt
    10381 ctatgggacc tccggcgatc gaatccttct gtggtttgat ctcatcaacg ccgcaggcgg
    10441 ccgtgctccc atgggcagcc agctcgagct cgatggagtt ccgaagttca gagtccgcgc
    10501 ggcgggcgcg ttcgagcagc tcccgggttg ttcgaaagaa gtgatggaag ctctcggctc
    10561 agagcggatc gatcgaatct gcgcaggcga atgctatcac ccgtcggatg agcgccggag
    10621 catcacccgg atcgaagtcg tacgaatcag ccgccagatg agtgaagccg aggccgtcga
    10681 ggacttgatc gaagaccctt ggctcacaat cccatgcccc agcggccaag aggtctgcga
    10741 ggtcgagttc gaagatcctt ggtatccgtc ggccggacgc gaggtcgtct actacgttcg
    10801 cgccattcag gagccaaccc ccgccgtcaa cgccggccag cttcgctgca ccggcgacga
    10861 gtgcaagccg tgctacgggg actatcgcgt atcgttcgac gacgactgct tagccatgga
    10921 ccaggagcgc gcctggtcgt cgccgatcta cctcaccccg cccgggacgc cggctcctga
    10981 atagccctag cggtcgtcgc agtccgcggc cccgagggcg cgcaccttgc gcagctcttt
    11041 tttcgcctcg gcagccaact tcgagctctt gtggtcgttc agcagcgcct tcagcgcggt
    11101 gcccgcatcg ttgcacgagc gaacttgcag aaacgcctgg gccatgtcgt acagcgtttc
    11161 gtcgatggcg tcgccctttc ggtagttcga caataccttc tggaactccc cgagcgcggc
    11221 tgctgcctgg ttctgcttca agtagctcga tccgatcatg tactgggcgt tgtccgcgtt
    11281 ctcgtccttg gggtagcgtt cgacgtaggc cctcgcgagc gcacgcgtgt acgagtattt
    11341 gttgatgcgg taggctttcg tcagcgcatc ccagtgctca cccttgttct tcgggatatc
    11401 ctccgcgtcg atggccacat ccatcccggc ggctcgtgca acgtcgttca agcgctgttg
    11461 aagttcgagc gttcgttgcg cttgcgcttg gctcgaaccg gcgctctcca tccggagctc
    11521 ggcaacctga ccctcgatga cggctagcgc ttgctgaagc tgctcgatct ggagtccttg
    11581 gtcggcgctg ttacgccgca gaatgcggct ggcctctgcg agcgcttcct cgagctcctt
    11641 gcgtttgacc tcgaggtcgt tttcgagcga gtcgagtcgc tcttgattgg ttgcgatgtc
    11701 ggcccgcatc tgactgatgc agcctggcgc ggcgccgagc cacagtggta ccgcgatgag
    11761 accggccacg agcgccggtc gcgtgcagcg gagttgttgg aaagtcccca ttgccccctc
    11821 atttagtata gatgggttac tcaggccatt ttaagagtga gggtgaagca gcccgttctc
    11881 gttgatgatc ccctgcagcg cccccaagcc cttttgtagt tttgcgaaga aggatgggga
    11941 atgcggtacg aagttgaaat tggggtgttt cgaccagagc tctctgaggc gcatgtccag
    12001 ctgcctggcc tcctcgttcg tctccgttcg tgcggggttt ccgccctcga tcgatatgtc
    12061 gcccactgcg gccgactcga agaagatcac ggcgtcgtat cgcaggagct cgtcactcag
    12121 cgtcgtcccc aaagtctcga agaagccctg aggagccgac tccggccaga acgcggcgcc
    12181 gtcgatcgta ccccggtcgc agagttggac ccggcccggg tagagcgcgc cctggatgtc
    12241 ttcgagcgca acttgggcgt gaaaaatcgc ttgctgagtt gccgcgcgcg ccctgggctc
    12301 accgccccgc ggaaaccctc ccgtgaatag catcgtcgca gtctcgggga cgatcacgat
    12361 cttgtctccg atctcgcggc ggaacaggtc tgcggccgtg gttttccccc cacccgggcc
    12421 acccgtcagc acgattcgat agtgatttct gagctgacca tgggtcaccg gtcgaagata
    12481 cccgattcct ggtcctacgt gcacccgaaa gcctttgtgg attttcgcgg atcggtggta
    12541 tcgacggcac tcgatgagca gcgaggaaca tgcgccaaga acgctgctgc gaggcggttg
    12601 catcgtcgat ggtaccggag cagctgctcg gtcgggaagc gttctgctcg agggcggtcg
    12661 catcgttgct gtcggggatc ttccggccga tgcgggagca tctatcgatt gcgccggcaa
    12721 ggtgatcgcg cctggcttca tcgatgcgca ttcgcacatg gacttcttct cggccggcac
    12781 ggacccacac catttcgact cctttgcggc gcagggcgta accacgtttg tcgccggaaa
    12841 ctgcggattc agcccgttcg gctttgcgcg caacacgaag catcgcggcc tgctcgagaa
    12901 ttccctgttc aaggccggcc gggagtctct ggattggaac agcttctcgg agtaccgggg
    12961 cgttctcgac gggctcggtc taacacagaa cttcgttcac ctggtcggtc acggtgcggc
    13021 ccgaacctcg ctctccggat tcgaggcccg ggggctgaac gcatctgagc gcgccgaaat
    13081 gatcgcgctg ctcgacctgg cattgagtga aggcgctgcg ggggtctcat tgggacttca
    13141 gtacaagccg ggcgtcttcg ccaagctcga cgagctcgag gaggtcgctc gcctcgtcaa
    13201 acggcacgac aagctgctca ccgtccacgc aaaagcgtac tcgtcgtttt ccggaacgta
    13261 tccgatggtc cccttcggac gggcgcacaa tctcaaggcg atcgacgata tgctagggct
    13321 cgcacgccga accggcgtca ggcttcaatt ttcgcatttg atcttcgtcg gatcgcagac
    13381 ttggaagacg gtcgacgagg cgatcgagct cttcgatcga gcgatcgccg acggcgtaga
    13441 cgtttgcttc gacatgtttc cctatgcctg tggagcgacc ctgctcaata ccttgttgcc
    13501 cgagtggttc atggcgcgga tgcccgaagc ccttcacagc tcgatcgcca gggcgaggct
    13561 gcgcgtcgag gccgaggttg gtttccggct ggtggggttt gggtacccag agatccagat
    13621 catcaacgcg agctgtccgg agtacgatga gcacaacggc cgttttcttc cggagatcgc
    13681 cgagcgcgtc gggaaatccg acttcggcac gatgctcgac attctcgaca agagcaacgc
    13741 cgaagcccgg gtactcttcc acaagtacta caatgacgag atcatcgacc ggctgatgcg
    13801 gcatccggcg gcgatctttg cttcggattc ctggcccgag cctcgggggc accagaatcc
    13861 tgcggcgtac ggaacgtttc cgagattcct tcgcaaggcg cgagagcggg gagtcgtttc
    13921 tctcgaggag gccgtccaca agatgaccgg cgcagcggcc gatcgattca ggctggagcg
    13981 gcgagggtgc gtgcgcgaag gctgcgcagc cgatatcgtc gtgttcgatt ggaacctggt
    14041 gcgcgacggc gccaaccgga ccggggacgc cgccccatct ggggtcgagc acgtgttcat
    14101 caacggacgg ccagcaattg aagctggcgc gattcatgcg gtgcgtggcg cgggcgaggt
    14161 gctgctgtca tagatcttct gcgcgcagcc acacgctcag tcagataacg atgcgaattt
    14221 ctacggaaag ataccgtcca gcgtgtaggc gttggcgacg ctttcaatcg cgccgagata
    14281 agcggaggta cggaggtccg ggagcgctct tctcctccaa ggctcgcgga tcttctcata
    14341 cgcgatcgcc attgtgttat ccagagccgt gcggacgaag tcgatttcgt tgggcgcctg
    14401 gagaaggagg gccgcctctt ccgggggcag agtctttccg gtgaggcgac tgatcgctcc
    14461 aagcagccgg ttgttggaga tctgctgata gcgccgggtc attcgttcga agctcacgtg
    14521 ggacaggttc ttgatccatt cgaagtagga aaccaccacc ccgcctgcat tcgcgtagat
    14581 gtcggggagc actacggtcc cggattcgcg aagaaaccta tccgcttccg ggtctattgg
    14641 tccgtttgcg gcttccacga tcaccttggc tttcagccgc gaagcgttgc gcatggtgat
    14701 ctggtgttgg agtgcagcgg gaatcagcac atcgcatggc tcttggagaa tcgcgtcagg
    14761 gtcggcgagg tatcgtgcct cctgaaaacc ccggagcgag ccggtctcgg cacgatgcag
    14821 caagacggcg tcgacgtcca gcccgccttc gtcgtagagt gcgccgtcac tgaccgacac
    14881 tccgacgatg actgcgtccc gctgtaccgc agcgagcgcc gcgtggagcc cgaccttccc
    14941 taggccggtg acgatgatgc gttttcccat caacccgact tccagaccga gcggcttcac
    15001 atcttccgct tgcgatagga agtactcgag cgcgatgatt gcgcccagcc ccgtcgcttc
    15061 acggcgcccg gcgacgccat gcaggctcat cgcttttccc gtgacgcagg caagcgagtt
    15121 caacgtatct ggcccgtagg ctttgtacgt gtcgtaaatc caacccattt cgcgctcacc
    15181 ggtgcccaca tcgggcgccg gcacgtcgac gtcgggacca atgaagcgct tgcgaatcag
    15241 ctccgcggtg tatcgacgcg tcaccctctc tagaaacgaa gccgactcgt tgcgagggtc
    15301 gaggcagaca ccacccttgg caccgccaaa aggcacgtcg accaccgcgc acttgtaagt
    15361 catgagcgca gagagggcca tgatctcgtc ctgtgaaatg tcgagcgcgt aacggatgcc
    15421 gcccttggtt ggcagccggt gatggctgtg ctcagcacga taagcctcaa ccacgacgac
    15481 tcgaccgtcg tcacggcgca caggaaatcg cattcgatag accgcgttgc acgcgcgaat
    15541 ctggccgagg atgtctggag cgacctcggt atgctggcag gccgcgtcga agtactggcc
    15601 gacgtcgctg aagaagctgg gactcggaac catgcacgac gatagcgcgg aatccgacca
    15661 caaaacccca agaaaaggca gcttgacagg caccagaaca atgatattga aaatcacatt
    15721 caataccatg cgcaggcgct gcgctctctg ctgcacttag aaatgcttgg gagagaagag
    15781 ggtgatgacg aaaatgacga agtcttggtt ggtcatgggg atgctcttag gaggctgcgg
    15841 cggcagcgct gatagcgagg ccggaggccc atcgacggcg gccctcgccc aagaagccac
    15901 atccacgtac gcgcgaattg cgcaccaggc gtactccgac tctcttgcca ccgccgtcat
    15961 gttgaccgac gcactcgatg cattcgtcgc tgagccatcg atggaatctc aggaggcggc
    16021 caaggccgcg tggcgggccg cgcgagagcc ctacctgcag accgaggtgc ttcgattcta
    16081 cgacgggccg atcgacaatc ccaccgacgg tccggagatc tacctcaacg catggccgtt
    16141 ggacgaagcc tacgtcgact atactcagga tgccgcttca gcagggatca tcaacaaccg
    16201 cgacatcccc atcgatgaag ccgccctacg tggcgccaat gaagaaggcg gcgaggagaa
    16261 catctcgctg ggctttcacc cgatcgagtt tctgctttgg gggcaggaca ccactgatcc
    16321 ctcagacgag ctccccgggc tgcggtcgtt caccgactac ctcatcgaag aaggtggcac
    16381 tgcggagaac caggatcggc ggggcgcgta tctgtcgctt ctcgggaacc tcttggtcga
    16441 tgacttggag aaactcgtga acgcttggag ccctgacgat gccggcaact accgagccgc
    16501 tttcgaaggc ggtggatggg agcccgcgct cgaaaagatt ctcactggca tgatcatcct
    16561 cagtggcttc gaggtcgccg gtcaacggct ccaggtggcg ctcgacaacg gagaccaaga
    16621 ggacgagcat tcctgtttca gcgacaacac acaccgggac atggtccaag acgttcgggg
    16681 cgtgcagaac gtttggctcg gcgagtacca agcccttgat tcgacccagt cggtttcggg
    16741 taccggtctt catgaggtcg tcgcgtcgat cgatggcgcg ctcgccgcgc aaatcaccca
    16801 gcaaatcgcc caaagcgttt cgctggcgga agcccttcgc ccgacggagg ctgctccacc
    16861 gttcgatctc ctgatctccc tcggaaacag cgagggaaac cagaaagttc aggcgctgat
    16921 cgattcttta cgtacccaag aggacttgct ctcgagcgca ttcgtcgcct tcgggctcag
    16981 cgttccagag ccatagtgaa aggaccagcg catgacccgc tgcaccgctc ttggcgtcgt
    17041 tctcgcgatt tcgctattcg gctgctcggg gggctccacg agccccaccg cagatccagc
    17101 cgaggccttc cccggcggcg atacgacgac tttctttctc ttcgcgggta acgagctcgc
    17161 gcaaccggct gccaatttgg aagatcgctc gcccttcttc acaggggaca gcctgttcaa
    17221 tcagacctgg gtcacggcgc cagcatcgac cgccgctcga gacggtcttg gaccgacgtt
    17281 caacacgcgc tcttgcgcgg gttgccattt ccgagacggt cgaggccgtc cgcccctcca
    17341 agatggaggc acgctcgagt cgatgctgat ccgcatcagc attccagggg aggacgcgca
    17401 tggaggaccg ctcggtgacc cgagctacgg cgatcagctc caaatcgaca gtctcgcggg
    17461 tgttccggcc gaggtgaagg tccgcgtcta ctgggaggag cagcaagggg catacgaaga
    17521 cggcacgccg tacacacttc gcacaccgcg atacgcgtac gaagacctgg cgttcgggcc
    17581 attggcggac gatctccggg cgtccggccg cacggcacca cacatgagcg ggctcggctt
    17641 gctcgaagcc attcccgagg aaacgctttt ggactgggcc gatcccgagg acgccgatgg
    17701 agatggcatc agtggaagac tgaactgggt ttgggattcg gttgcccagg cgatgcggcc
    17761 ggggcgcttc ggatggaaag gcgaacaacc tacggtccga cagcaggtcg cgggcgcact
    17821 gcttggcgac atgggcatca cctcgccctt gtttctggat cagaactgtc ctgcgccaca
    17881 gattgaatgt gctgcggctc caagtggcgg ggagcccgag attggcgaaa gcaacttcag
    17941 caagctcgtc ttttacagct cgaccttggc gcctcctacg agggatgact acgacgaccc
    18001 gatcgtcctt cgcggccgcg aatggatgcg cgagctcggc tgcacgagct gtcacaaaga
    18061 gagcgctaag accggtgagc atgattcgat ccccgagctc tcgagccaaa agattcgtcc
    18121 ctacaccgac ctgctcttgc acgacatggg cgaaggcctg tcggacgagc gacccgtgtt
    18181 tctggccgag gggcgtgagt ggcggacgcc accgctctgg ggcatcggac gtgtgaaggt
    18241 cgtcaaccag cacgattttc tcctgcacga cggacgggcg cgcgggtttg ccgaagcgat
    18301 cctttggcac ggaggtgagg cggaggaagc caaagacgcg ttccgaagtt tgccattcga
    18361 agagcgcgaa tcactgatcc gtttcttgga gtcgctatga acacgcccgt gcgcatcgtc
    18421 ttgctcttgg ctgtcgtctc ggcggcagtc gcaggatgtt tcaacgagcc tttcgatcag
    18481 caagccatgc ttcactcact cggcacggat gtgatcattc cgagctatgc gtcgttctcg
    18541 gaacaaaccg agacgctgct cgtttcggcc agagcgttct gcgagggtgc tacggcctcg
    18601 cgactcggag atcttcaagc tcggtggaag gaggcccgtg tccactggaa gcggacggac
    18661 gtgatcaact tcggcccata catggaacag ccatggagat tgggccccaa gatcgactcc
    18721 tggccggttc gcgagaccac ggtcgacgag aatctggccg gtgaagaacc gctcaatttg
    18781 gaatccgtga gcgcgcttgg tgcggccagc cgcgggcttc ctgcgatgga gtacctcatc
    18841 ttcgatccag cgggctcggg agtggcactc gcgaagttcg agggcgccga gggcgagcgc
    18901 cgctgtgatt acgtactcgc gttggcggag gatctccgtg ggagcgccga ggcgatggtt
    18961 gaagcttggt ccccggaggg cgacgattat ctgggcgccc tcttggcatc cggcgaaccc
    19021 ggtgcgccat tcatgtcggt ccgcgatgcc agcaacgaga tcgtaaatcg aatggttttc
    19081 ctcgtggaga acatcatgcg cctcaagcta gcccggcctc ttggcttgga ggcaggtggc
    19141 gacccgcaac ccaatgaagt ggagtctcgc tacagcgatc actccatcga agacattctc
    19201 gcgaacctcg acgggctcga gaacatctat cgcggccgat acggtgaccg ccgtggtgtt
    19261 ggcgtccaga cgtggctccg ctggggcagt ccgccgatcg ataccgagat gatcgcagcg
    19321 atcattaggg ctcgttcggc tgtcgaagcc atttcagagc cgctcagtcg tgcggtcgag
    19381 accgagcccg cgctcgtcca gactgcgtac gaccaattgc gcgatctcag aaacaccatt
    19441 ggtgtcgacg tgatcaatgc gctctccgga agtgcgacgt tcaacgatac cgatagtgac
    19501 taggcccaac ctcgagaaac tgagggctcc agtcgacggc gccagcctgg gcgtattccg
    19561 cgccggattc gggctcatga tgctcgtcag cgtggttcga ttctggtcca agggctggat
    19621 cgacgagatg tacatccaac ccgcgctgtt cttcccgtac tgggggttcg agtgggtaga
    19681 gccctggtct gccgagggga tgtatctgca cttcgcaata ctcggcgcct tggccacgtt
    19741 gatcatgatc gggctatgga cgcgcccggc gctcttgctt ttcttcttcg ggtttaccta
    19801 tgtggagctc atcgatcgta cgacttacct caaccattac tacctgatca gcgtcgtatc
    19861 gcttctgatg gtgttccttc cggtcgagcg ctccttctcg ttgagtcgag cactcggtcg
    19921 taccaccgga cccgaaaccg tacctcgatg gagtgtctac acgctccgaa tgcaggttgg
    19981 cctggtctac gtgaccgccg gtcttgccaa gttgcgagct gactggctct ttcgcgccga
    20041 gccattgcgc acttggctca gctggaacgc agacatccca gtcgtgggcc cgctccttgc
    20101 ggagccctgg gtcggatttg cgatgagctg ggcgggggcg ttcttcgacc tgttcatcgt
    20161 ggcgtttctt ctttggaggc cgactcgcgt ctatgccttc gtcgccgtcg tcttgttcca
    20221 tgccttcacc ggagcgctct ttccgattgg ggtctttccc gtcgtgatgg cgctcagcgc
    20281 gaccatcttc ttcgagcctg actggcctcg gcgattgatt gggccgccaa gcgccgttag
    20341 ccagacacca ccccccgcat tggcgccgct cggacgatgg cagaagctgg gttttgctct
    20401 gctcacggcg catttcgtct tccaggtcgc cttcccatgg cgtcagttgt tgtatccagg
    20461 aaacgcctgt tggacagagc aggggtttcg actttcttgg cacgtgatgc tcatcgagaa
    20521 gaccggacag gtcgactttc gcatcgtcga tgagccgagc ggtaagactt ggctcgtctt
    20581 cccgcgtgaa aggctctcgg cttttcaaca tcggatgatg tccacgcagc ccgacatgat
    20641 tctgagcttc gctcaccatc tccgcgacga gtggaaagcc gagggatatc cgaacgtcgc
    20701 ggtctatgcc gacgcctggg cgtcactcaa tggtcgccga cgccagcggc tcgtcgatcc
    20761 cgccgtcgat ctcgcgcgcg aggaggaaag ctgggccaac aagacatgga tcgtgccatt
    20821 ggaggacact gtggaggcac gcagagagta gtgggtttga caggaacagc gtcagtgcca
    20881 gcgtcaaggt caatgtcagt gaaggcgtca gtgacgatcg gggtgttgcg ttgagcgttt
    20941 cgttgacgcg aaccgggcct ctcctcgcct accagacttt tggccgccga ggccccccag
    21001 tgctgttcat catggggttt gggatgccgg gcgcagtctg gagtcctcag gtcgacgagc
    21061 ttcagagcga ccaccgatgc tgccactacg accacctggg cgtgggcgcc agtgagcccg
    21121 ggccgcttct tcggacaatc ccggcaatgg ctgacgatgc ggtgcgcatc ctcgatgacc
    21181 tttcatggga ccgggcgcat gtcgtcggcg tctcgatggg aggcatgatc gcgcaggagc
    21241 tcgctctgag gtccgccgat cgatgccaaa cactgaccct catcgccacg cacggtggcg
    21301 ctcccatcgc ctcgcttcca accctccgag gaatgggcct cttcctcaag gggctcttcg
    21361 gcggccccaa ggctcgcatg caatctttgc ctcgcttgct gtaccccccg gaatttctcg
    21421 agacgatagg ggccgacggg cttcgggcgc acctcaaggt gcggctcggc cagccggcac
    21481 ggctgcgcac cgtgctgggg cagctatttg ccgtctggag acactctacc gaggctcgcg
    21541 tctcgcaaat cgacataccc acacttctcg ttcgtccagg gaaagacatc ctcatccgac
    21601 cgacacaaac cgatcgcctg gccaagcgca tcccgaacgc gcgtgtcctt cgattcgacg
    21661 acgcaggtca cggcgtgacg ttccaaaagg cctcggagct gaacgctgct ttgcgtacgc
    21721 acttcgccga ggacggcaac cggcgaccga aagccagctc agcgccacgt gcaagagctt
    21781 agagcgcgag atcgatggag tggagtgcga ccgtcgagac gcgtgtcgcg acccggctca
    21841 ttctcgcgtc ttcaggacgg cgatcagatc gagtcggtcc agctttctcc gtaccaagag
    21901 cgcgctcgcg attccggacg ccagaaccac agccacggaa aaagcgtagg tctgatcgga
    21961 gatgatgaac gggattctga agatctcggg gtccgcccag gatgcgatga gcattttggc
    22021 tcccagggta cccagcaaga gcccaagggg aatggcgatg gcgatctgca cggcctgctc
    22081 gcccagcaag atcgccgaga tctccccgcg tgtgaagccg agcacacgga gcgtggcgag
    22141 atcccggcct cgcatcgaga cgctcacgcg cgcgttgttg tagacgatcg agatcgcgat
    22201 ggcggtagca aacaaggtca tgatcaacgt catgactttc atcgtctcat cggtctgctt
    22261 ttcgaagctg gcgatcgcgt cgctctttcg agacaccgcc tgaatggccg gaatctcgtt
    22321 gagacgcgac tggacctcag cctccgtggg gacgtcgatc cgtagcaagg ccgtcgagaa
    22381 cctagggctc tctctcagga gccgatggag gttgtctttt gacaggtacc cctgcagacc
    22441 gaagccttct tcgatcagcc cggctacagt gagctctcgg gtcccccggt ctccctcttt
    22501 tagctcgatc tgaacgacgt ctcctggttc cacattcaag atctttccga gaacgctcgt
    22561 cagtagaaca ccgtctttcg ggattggaac gttcttgaac tcgaggttga acagttgtcg
    22621 cagccgcgag gcatcgggga tccccatgag catggtatcg cgccaatggt gcttggatcg
    22681 catccgaacc ggcgtcgctc gaattccttc cacttcctcg actccgggca cgtgtgccag
    22741 atacccaagc ccacgctctg gcatcgcatc tgaaaagctc acggtgaggt cctctcgctg
    22801 ctgacgctcg aactgaacca tcatcatggg cccgtatgcg tcgtatccga atcgacccag
    22861 gatcacgatc gacaaagcga gggcgatgcc aagcgacgaa agcgccgtcc gtccgggact
    22921 tcgccagatt tcgcgaaaca ccatcagcgc gggctgactc atccacttgg tcatccccca
    22981 tcgttcgagc gccgcggtcc ggtacttcgc tggggcggga ggccgcatgg ccgccgcggg
    23041 cggaagcttg actgcggcgc gcacggacat ggaggctccg agcgccccgg ctatcaggct
    23101 gaagagaatc gcgttgatgc tgacactcag gtcgactctc tgcggcattt gcggcagccg
    23161 gaagaactcg gcgtacatgg cgagcatccc cttgcccagc cagactccga ggccaaggcc
    23221 gatcaacgcg ccgaagctca cgatgagcgc caccattttt aaatagtgcg tggcaatcgt
    23281 cgcgtttctg tatcccattg ccttcagcac tgcgatttgc gggcgctgca gcgcgaccaa
    23341 gcgactgaac acgacgttga gcaggaatgc tgcgacgccg aggaaaacag taggaagaaa
    23401 gatcaccaac gtttcaatcc cttggatctc gctcgtgagg accgaatgcg agagttggtg
    23461 ctcgcgtgtg taggctccga gtccgccgta cggcttgagc acacggtcga tcgccgaaag
    23521 gacactgtcc tgattggctc cgggctcgag cttgaccgac aagtcgttga atgcgccttc
    23581 catccgatac gcggccgcga tcgcatcctc gagcatccag agaggcgtga accgctcgtc
    23641 atcgggcatg agcagccccg agtcgactgc gaacacgtat tctggtgaaa gtgcgtgacc
    23701 cacgaccagg aggtcacgca acttcccatt gatcactgca gagatgtgat cgccaggctc
    23761 caagccgtgt gctttggcga acggttcgtt gagcagaact tcgtcgtatc gaccgggctc
    23821 gaggttgcgc cccgcccgga ggacgagccc attcatcggc gccctgccag tggagggcac
    23881 ggagatcagg tcgccgcgtg ccggcgagat gagatcgtcc atcggcaagc ggacctgctc
    23941 cacgattcgc gggtaggcgc gggcgacccc gggaagctcg gacacctgtc cagccaccga
    24001 cttgggagct cgttcgaggt gcacgaacac ttcagcgaag tgattcgtct ggtagtagag
    24061 gtcacgggat tgatagaggg cgcgccaagc gctttggagc gcgatgtaac aggctacgcc
    24121 agacgccacg actattgcga tggtgagcgc ctgcccgcgg agccgtacga cttcgcgcaa
    24181 gagctttcga tcgagagcct tcaccggttc gtgctccgca gcgctcgccc catcgaagcg
    24241 atctgggcgc gcatcttcca gggccaccgt gaacccggat tcaaccgagt gtcaagcgcc
    24301 gctctgatgt atagtgccgc gggtgaggtg ccccccaatc gttgtcatcg tgtcgctgct
    24361 tctgggctgc aactcgatgg gcagtggcga ttcagcgccc gtgcaactcg gagtcgttcg
    24421 aacggtggac gaggcaaggc tcgaggaccc cggcaattgg ggggatgagc tcgatctcgc
    24481 gcttgaaacc gagttctggg atttgatcgg caactccgat catgctcgct accccgcttg
    24541 gcactcgaaa gccaaggcgt acgtcgctgc aaacttcgat gccgacccgc gtcttggaat
    24601 gctgatctcg gccggcgccg tcttcagtct cactacgcta tttcaatacg acgatccgct
    24661 ggcacacctg gccgagtact ccgactacgt cgccgatgcg accgtaggca tccacgacgc
    24721 gattgagcct ctcggcgacc atgagcccgc gctcgtcttc tttcacaaca accaggccat
    24781 gctcgcgttc ctcttgagcg agaacgcgga cctcggaatc tgccacctcg agaagcttcg
    24841 caacctcgag gacagcaatc ctacgtttca cggtaccgag ggaagggcag cggcgccgtt
    24901 cacctatggc atggtcgacg atccagcgta cgtggattac gggatcgcca tgatggaagg
    24961 ctgcaacaat tggatctgca actggacatc caagctggcg ccgttcaaac ccataggcca
    25021 actcatgacg ctcgcggagc tatacg
//
LOCUS       DASRZF010000082         7504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3499594, whole genome shotgun sequence.
ACCESSION   DASRZF010000082 DASRZF010000000
VERSION     DASRZF010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 7504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7504
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3499594"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            311..460
                     /locus_tag="VFG22_03625"
     CDS             311..460
                     /locus_tag="VFG22_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415363.1"
                     /translation="MKSQTARPGLSAGAPWVAVQELTPAEGDLQRCAVERDANPLPIL
                     NPCED"
     gene            complement(897..1229)
                     /locus_tag="VFG22_03630"
     CDS             complement(897..1229)
                     /locus_tag="VFG22_03630"
                     /inference="COORDINATES: protein motif:HMM:NF016626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF615 domain-containing protein"
                     /protein_id="HET6415364.1"
                     /translation="MSKKKSRLRSTTAEEEVIYESRSSDRAADRAEKATMGRLVRSIA
                     QLSPKQRAKVGLDEQLMAEIERLAGMPVKSAYGRQVRTITSEMRRLGLDEKQVQAAYE
                     RGRGAGRG"
     gene            complement(1584..2084)
                     /locus_tag="VFG22_03635"
     CDS             complement(1584..2084)
                     /locus_tag="VFG22_03635"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET6415365.1"
                     /translation="MPKLTPRNTDFVDTAPIRITTVVTAKATPEEVWAVMVDNERWPE
                     WFNAAKSCRTTSDVSGGVGSTRWIHVDLFKVNERFIAWDPPHRWSFTILDVNLPGVVS
                     VVEHALIEPVGTRETRVTYVMASEVAPYLRPLVPILRWRLTGMFKKGLAGIEGQVVRL
                     RKEAQT"
     gene            2485..2721
                     /locus_tag="VFG22_03640"
     CDS             2485..2721
                     /locus_tag="VFG22_03640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HET6415366.1"
                     /translation="MTSATKKILEDALALSEEQRATLVAALNESLETAEDELTPQWKA
                     EIARRIEAVERGESRLIPGDEVEARIRKTLREIS"
     gene            2718..3026
                     /locus_tag="VFG22_03645"
     CDS             2718..3026
                     /locus_tag="VFG22_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415367.1"
                     /translation="MKLPFEADAADEFEAAATWYERERTGHGNLFASAVRGSVDRAAR
                     FPKSGIRAHGFDRERDVRRFSVLRFPYSIVTAIVDGRRAVVAVAHASREPQYWYDRVK
                     "
     gene            complement(3109..3184)
                     /locus_tag="VFG22_03650"
     tRNA            complement(3109..3184)
                     /locus_tag="VFG22_03650"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3148..3150),aa:Glu,seq:ctc)
     gene            complement(3229..3302)
                     /locus_tag="VFG22_03655"
     tRNA            complement(3229..3302)
                     /locus_tag="VFG22_03655"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3268..3270),aa:Gln,seq:ctg)
     gene            3548..5791
                     /locus_tag="VFG22_03660"
     CDS             3548..5791
                     /locus_tag="VFG22_03660"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HET6415368.1"
                     /translation="MTSWKEQLGDRIPEQLAGEVDLFEGQIELKKQGKLEDKIFAETR
                     LRRGVYGQRYDNGLRDDGTGQKTLAFPCGDLTKGPETVWDAPGMMRIKIPFGKLTAEQ
                     LEVLSELAEENSDSILHVTTRQDIQLHFIHIEEAPDMMRRLAAVGITTREACGNSVRN
                     VTACEYAGVCTTQAFDVTPYANAITQFLLGHPDVQDFGRKFKIAFSGCEDNPCGLVTF
                     HDLGAVAHVREGKRGFRVVVGGGLGAVPVQAKVLAEFCPEEELLPLAQAVSRVFARLG
                     EKQSRARARIKFLVQKVGIDEFKKLVAAERETLRPDERWTAFLHDLHATDEKPVREAG
                     AIPSKAPADFRAWAEHNLKPQAQEGYYSAIVKLPLGDFTSTQGRALAELARKYTGDSI
                     RCTVEQNLTFRWLSGADAVAFYEGLRALNMAAAGAGTITDMTSCPGTDTCKLGISSSR
                     GLTSELRNRLTQIEDQLDPAVKALRMKASGCFNSCGQHHAADIGFTGVSRQVGGRKVP
                     HFNIVLGGQWTENAKSYGLVVGAVPSKNIPKAVELITAHYLASREGDESFQAFIARVG
                     KREFRQVLAPIQKPPAYEDDRSYYSDWGDPREYTIGDIGVGECAGEIVPFVEFGLQEA
                     EQQLHDAQDALESGQAAAAAAAGFNAMVTAAKALVRHLEIQVKDDADDVVANFKTHLH
                     DTELFHDPFAKGKFAAYLLKLHDDRSYEAADSEIAHRTLDEAQLFLEAAHACYQRLTQ
                     AAAAAAE"
     gene            5797..6360
                     /locus_tag="VFG22_03665"
     CDS             5797..6360
                     /locus_tag="VFG22_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415369.1"
                     /translation="MQASKFQVKVYAKAGHIELEKLVPVFHEWIRAKKISDELLIDVA
                     DYAHVPQGPGVVLIGHQSDYYLDVADDRPGLLYSRKRGFEGDFQAGIDDAFRRALNAC
                     RLLEQESGLGLEFATGELLFRVQDRLKAPNEGTTYDAYKPVLEKTATAFFGKAPSLER
                     IGTQREPFAVRIDTGSTVSVAEALARH"
     gene            6429..6941
                     /locus_tag="VFG22_03670"
     CDS             6429..6941
                     /locus_tag="VFG22_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415370.1"
                     /translation="MTHPRLVVLLVLSLLTATACSRGSKSETDGNSEQAGVRGNTAAI
                     NTVRAAAHAMAQPEPRVDFVAAAMEGAITARTRSKALIHYDGYRATLTTPTDRVTQVT
                     FELIDAKPTIRQLTAELGEPEPNPKGMLYTYEYSPTGATIRVLAEPVSMPAEEDSLVR
                     RIVIEGARTR"
     gene            complement(6949..7338)
                     /locus_tag="VFG22_03675"
     CDS             complement(6949..7338)
                     /locus_tag="VFG22_03675"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HET6415371.1"
                     /translation="MPVVVAAAVLIRRGRVLLTRRAEGQHLAGMWEFPGGKLEQGESP
                     EAALARECLEECGIEIDVGEILDVTHHRYRERDVLLLFYRCVLRAGEVQNLQVAEHVW
                     VTPEELDGYSLPPADLGVVAKIQSLEL"
BASE COUNT         1509 a         2329 c         2307 g         1359 t
ORIGIN      
        1 ctcgcccatg tcgaagagcc ggtacttgag tgaggagctt ccggagttga gaactagaac
       61 gttcacgacc tgctcttgta cgtcgcgaga agatttcgaa caagagggcg cgcttgttag
      121 gagacggttc gtggtttggg cctaggacgc attggcggca cggtactcct tctttctccg
      181 ttacagcgtt ggcagcttgg acttagcccg cttccgtaga cacctggcta ggttgctggg
      241 gacggcctcg acggccgccg gactcatgtc tccaacgtgg ctgtggcgct ttcgatcgta
      301 gaagacctcg atgaagtcgc agacggcgcg ccccggcctg agcgccggtg cgccatgggt
      361 cgccgtgcag gagctgacac cggccgaagg ggacctccaa cgatgcgcgg tcgagaggga
      421 cgccaaccct ctcccaatcc tgaacccgtg cgaggactag cgtgcgcgca ggtgacacac
      481 ataggccaca gcgcaccaag ggacgctgac cgaagtcaga atcccttgtt tttccggtga
      541 ccccaagtac ctggagttgg aacgaatttt ccgctttcat tgaagagtgg gacaagctgc
      601 ggagggccgc atgacgagct tcgacgacga cggtcggccg gaccggagcg cgacgcacga
      661 cgataacgtc gataaacagt gcagcggcgc caccggcctt gggaaggtcg ccctccaggg
      721 tctttacgtc gtaggtgagg ttctgtcctt caagacgggg attgctaatg gtcaccacca
      781 catcggtgac tcctccgtcg tgttccagga tcgaccgcac gccgggtgct tggcgagggt
      841 cgcacgatgg tcacgatctg caaagtcacg acaaacgcag ctgacaccgc ctgtttctaa
      901 cctcggccgg cgccacgacc acgttcgtac gctgcttgca cttgcttttc gtcaagacca
      961 agccggcgca tttcagaagt gatcgtccgg acctggcggc cgtacgcact cttaactggc
     1021 atgccggcga gacgctcgat ttcggccatc agttgttcat cgagaccgac tttcgcgcgc
     1081 tgcttcgggg acagttgcgc aatcgaacga accaagcgcc ccatcgtggc cttttccgcg
     1141 cggtctgctg cgcggtccga cgatcgcgat tcgtagatga cttcttcttc ggccgtcgtc
     1201 gaccggagcc gtgatttctt cttggacacg ccgcaccatg actcactcga aggcgacaaa
     1261 ctacgctatt gtggatttag cctctttatg gattcgttcc gggttcatcc gggcctctga
     1321 ttgcaaaagc agaagaccga ccgaagccgc gctctctcat ggcggtgacg ccaacgatgc
     1381 gaagttggaa cgaattctcc acctttattg aagagtggga ggagcttcgg agggccgctt
     1441 gatgacgttc gacgacggcg atccatgcac agctacgcgt attgcgttca gcgaaggcgg
     1501 ctaccgcgct ccacccgtgt atgtcgagcc atcggcaacc ctcgtaaggg tccgagtagc
     1561 tgtcggggag ccgatcgtcc gcctcaggtt tgggcctcct tgcgaagccg gaccacctga
     1621 ccttctattc cagcgagccc cttcttgaac ataccggtga ggcgccagcg caagatcggc
     1681 actaggggtc gaaggtacgg tgctacctcg gaggccatga cgtaggtgac ccgagtctct
     1741 ctagtgccaa cgggctcgat gagcgcgtgc tcgaccacgg acacgacccc tggcaggttg
     1801 acgtcgagaa tcgtgaatga ccaccggtgc ggcgggtccc atgcgatgaa gcgctcgttc
     1861 accttgaaca agtccacgtg aatccagcgc gtcgatccaa ccccacccga cacgtccgag
     1921 gtcgtgcggc agctcttcgc agcgttgaac cactctggcc agcgctcgtt gtcgaccatc
     1981 accgcccaca cctcctctgg cgtcgccttg gccgtgacaa ccgtggtgat tcgaattggg
     2041 gcggtgtcga caaaatcggt gttgcgcggc gtcagctttg gcatgcctca tcgtatgcct
     2101 gacgaagcgt tgtcgtccgg aagatctgac ccaggttgag cagaaagcgg gactatcacg
     2161 caacctgtcg acttcggtga cagataagcg cagccctcga ttgagcgagc cttcttgcga
     2221 agaaaaagcc cagccgaagc cggactctct gatggcggtg accccagcga cgcgaagttg
     2281 gaaccaattt tccgctttca ttgaagaatg ggacgacctt cggatggctg catgatgagc
     2341 ttggacgacg gcaatccgtg cacagctgag tcgcgcccgc ttgatcaggc tcgcgatgat
     2401 cgtgggtcac atctcacggc ggacagggtc ttgaggaacg acaggccact agcgatgcga
     2461 cactttcccc gttaaactgc tttggtgacc tcggcaacca agaaaatcct agaagacgca
     2521 cttgccctat ctgaggagca acgggcgaca ctggtcgctg ccctgaacga gagcctggag
     2581 acagcggaag acgagctcac gccccagtgg aaggcggaaa ttgctcggcg catcgaggcc
     2641 gtggagcgcg gggagtcgcg tctcatccca ggcgatgagg tcgaggccag aattcggaag
     2701 acgctccgcg agatctcgtg aagctaccat tcgaggcgga tgctgccgat gagttcgagg
     2761 cggcagcgac ctggtacgag cgtgaacgaa ctggccacgg caatttgttc gcgtcggcag
     2821 tgcgcggaag cgtggaccgg gccgctcggt tccccaaaag tggcatccgc gcgcacggat
     2881 tcgatcgcga acgagatgtg cggcgcttca gcgtgctgcg ttttccgtac tcgatcgtaa
     2941 ccgcgatcgt ggatggccgc cgagcagtgg tcgcggttgc gcatgcaagt cgcgagcccc
     3001 agtactggta cgaccgcgtg aaatagatgg cgccgtgact cagccacgcg gatgtcgact
     3061 cgagacatcg cttcaaggac aaagcccggc tgaaaccggg ctttgtgctg gtgaccccag
     3121 cgaggttcga actcgcgtta ccggcgtgag aggccgatgt cctagaccac tagacgatgg
     3181 ggccgaaagc taatcttgct acgttggctg cgttccttaa cagctaactg gctcggggac
     3241 taggattcga acctagatag ccagtaccag aaactggcgt cctgccatta gacgatcccc
     3301 gaatggtctc gacaccttag tgaggacgcc gagcggcggc actatagcgg caaggcaccg
     3361 ctcagcaagt gactggcgca gaatcctatt cttgactatt taagaggggt acaaatcgtg
     3421 atttttctaa taaaatcagt ccaaatatcg caatttagct attgttgact tgacccgcgg
     3481 taatgctgcg gaatagtgag caatcgccct tcggccgcaa ttcgagcgac tggggctgag
     3541 gagcatcatg acttcgtgga aagaacagct tggcgatcgt atcccggagc aactcgccgg
     3601 tgaagtggac ctcttcgagg ggcagatcga gctcaagaag cagggtaagc tcgaagacaa
     3661 gatcttcgcc gaaacgcgtc tccgtcgcgg tgtgtacggc cagcgctacg acaacggcct
     3721 gcgcgacgat ggcacggggc agaagacctt ggcgttccct tgcggggatc tgacgaaggg
     3781 cccggagacg gtttgggatg cgcccggcat gatgcgcatc aagattccat tcggaaagct
     3841 caccgccgag cagctcgagg tcctgagcga gctcgcagag gaaaattcgg attcgattct
     3901 gcacgtcacc acgcggcagg acatccaact tcatttcatt cacatcgaag aggcgccgga
     3961 catgatgcgc cggctcgccg ccgtgggaat caccacacgc gaagcctgcg gcaacagcgt
     4021 gcgcaacgtc accgcttgtg agtacgccgg cgtctgcacg acgcaggcct tcgacgtgac
     4081 gccctatgcc aatgcgatca cgcagttcct cctgggccac cccgacgttc aagatttcgg
     4141 ccgtaagttc aagatcgctt tttcgggctg cgaagacaat ccttgtggtc tcgtcacctt
     4201 ccacgacctc ggcgcggtcg cgcatgttcg cgagggcaag cgcgggttcc gcgtcgtggt
     4261 cggcgggggg ctcggcgcgg tgccggtgca agccaaggtg ctggccgagt tctgcccgga
     4321 agaagagctg cttccgctcg cccaggcggt gtcacgcgtg tttgcccgtc tcggcgaaaa
     4381 gcagagccgc gcgcgcgcgc gcatcaagtt cctcgtgcaa aaggtcggca ttgacgagtt
     4441 caagaagctc gtggccgccg aacgcgaaac cctgcgcccc gacgagcgct ggacggcgtt
     4501 cctccacgac ctgcacgcca ccgacgaaaa gcccgtgcga gaagcgggtg cgatcccgag
     4561 caaagccccg gctgatttcc gcgcctgggc cgagcacaac ctcaagcccc aggcgcagga
     4621 gggctactac tccgcgatcg tgaagctgcc actcggtgac ttcacctcga cgcaaggccg
     4681 tgcgctcgcc gagctggctc gcaagtacac cggcgattcg attcgttgca ccgtcgagca
     4741 gaacctcacc ttccgctggc tcagtggagc ggacgcggtc gcgttctacg aaggcctgcg
     4801 cgcgctcaac atggcggctg caggagccgg caccatcacg gacatgacgt cctgccccgg
     4861 caccgatacg tgcaagctcg gcatctcttc ctcgcgcggc ctcaccagcg agcttcgcaa
     4921 tcggctcacg cagatcgagg accagctcga ccctgccgtg aaagcgctcc gcatgaaggc
     4981 cagcggctgc ttcaactcct gcggtcagca ccatgccgcc gatatcggct tcacgggcgt
     5041 gagccggcag gtcggcggcc gcaaggttcc gcacttcaac atcgtgctcg gcggccaatg
     5101 gaccgagaat gccaagagct acgggctggt ggtcggcgca gtgccgtcga agaacatccc
     5161 gaaagcggtc gagctgatca cggcgcacta cctcgccagc cgcgaaggcg acgagagctt
     5221 ccaggccttc atcgcgcggg tcggcaagcg agagttccgc caggtgctcg cccccattca
     5281 gaagccgccg gcctatgaag acgaccgcag ctactacagc gactggggcg acccgcgcga
     5341 gtacacgatt ggcgacatcg gggtcggcga gtgcgcaggc gagatcgtcc ccttcgtcga
     5401 gttcggtctt caagaagcgg agcagcagct ccacgatgct caggacgcgc tcgaaagcgg
     5461 tcaggcggca gccgccgcag cagccggctt caatgcgatg gtcacggccg ccaaggcctt
     5521 ggtgcggcac ctcgagattc aggtcaaaga cgacgccgac gatgtcgtcg cgaacttcaa
     5581 gacacacctg cacgacaccg agctcttcca tgacccgttc gcgaagggga agttcgctgc
     5641 gtacttgctg aagttgcacg acgatcggtc ctatgaagcc gccgactcgg agatcgctca
     5701 tcggacgctc gacgaagcgc agttgtttct cgaagcggcg catgcgtgct accagcgact
     5761 cacccaagca gcagccgccg cggcggagta aggcccatgc aagcaagcaa gtttcaggtg
     5821 aaggtctacg cgaaggccgg ccacatcgag ctcgagaagc tggtccccgt ctttcacgag
     5881 tggattcgcg cgaagaagat ctcagacgag ctcttgatcg acgtcgcaga ctatgcgcac
     5941 gtcccccagg gaccgggtgt ggtgctcatc ggccatcagt ccgactacta cctcgacgtg
     6001 gcggacgacc ggcctggtct gctctacagc cgtaagcgag gattcgaagg cgatttccag
     6061 gcgggcatcg atgacgcatt tcgccgcgcg ctgaacgctt gcaggctgct cgaacaggaa
     6121 agtggcctcg gcttggagtt cgccacgggc gagttgctct ttcgtgtgca ggaccggctg
     6181 aaggcgccga acgagggcac gacgtacgat gcgtacaaac ctgttctgga gaagacggcg
     6241 acggcgttct tcggaaaggc cccttcgctc gagaggatcg gaacgcagcg agagccattc
     6301 gcagttcgca tcgacaccgg ctcgacggtc tcggtggccg aggccctggc ccgacactga
     6361 ttcgaaccgg gacctttttt gggcgctcct cgatttcgcg atacaaacct cagctaggag
     6421 ctgaagtcgt gacgcaccct cgcctcgtcg ttctcttggt actgagcctc ttgaccgcaa
     6481 cagcctgttc gcgcggttcc aagtccgaga ccgacggcaa tagcgagcaa gcgggtgtcc
     6541 gaggcaatac ggcggcgatc aacacggtgc gtgcggcggc tcacgccatg gcgcagcccg
     6601 aacccagagt ggatttcgtg gcggcggcga tggagggcgc gatcaccgcg cgcacgagga
     6661 gcaaagctct catccactac gacggctacc gggcgactct caccacgccc accgatcgcg
     6721 tcactcaggt caccttcgag ctcatcgatg ccaagcccac gatccgccaa ctcacggccg
     6781 agcttggaga gcccgagccg aatcccaagg gcatgctcta cacgtacgag tactccccga
     6841 cgggcgcgac gattcgcgtc ctggctgagc ccgtctccat gcccgcggaa gaagacagcc
     6901 tcgtccgccg catcgtgatc gagggcgcgc gcacccgctg aaccgggctc agagctcgag
     6961 cgattgaatc ttcgccacca cgcccagatc ggccggtgga agagaatagc catcgagctc
     7021 ctctggcgtg acccagacgt gctcggcgac ttgcaggttc tgcacttcgc cagcgcgcag
     7081 cacgcagcga tagaacaaga ggagcacatc cctttcgcgg tatcggtgat gggtcacgtc
     7141 gaggatttca cccacgtcga tttcgatgcc gcactcttcg agacactccc gggctagcgc
     7201 agcttccggc gactcccctt gttcgagctt gccccctggg aactcccaca tgccagccaa
     7261 gtgctggccc tcggcacgac gggtgagaag cacccggccc cgccgaatca gaaccgcagc
     7321 ggcgacgacg acaggcacct cagggcatct ccccaatcgc gcgaagcagc gttgccttgg
     7381 cgactcgaag cccaatggcc gaatcgacca catcgacctg cgcacgaatc agctcggact
     7441 gcgcttgcag caaagtggtg gtgttgacga tcccggcttg gagctggtcc cgcgtgacgc
     7501 gata
//
LOCUS       DASRZF010000083         7178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152 k141_59788,
            whole genome shotgun sequence.
ACCESSION   DASRZF010000083 DASRZF010000000
VERSION     DASRZF010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 7178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7178
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59788"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..975)
                     /locus_tag="VFG22_03680"
     CDS             complement(<1..975)
                     /locus_tag="VFG22_03680"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6415372.1"
                     /translation="MKSLSLFRPALIILALFAASCGSDSSSTNTSPPVPLELTASEAA
                     FDLSAVEAEYFEAIQYGPYDQNLFDIYLPASDEPTPLLIYIHGGGFTGGSRDTSESED
                     FVMSYLAEGVAFASIDYRLLQSPDPDGVIKSLTDSTRALQFIRYHSEQLNIDPDNVIL
                     MGGSAGAGTSLWIGFNDDMADPSSEDPVLRQSTRVTALIAIATQATYDIGKWTTVVFA
                     EYRLNLLELADALGLAQALLDFYGITDVDDFDSPAILEYRARVDMLDLMDADDPPFYA
                     QNDLEPAGPNAPPITVNLAFHHANHARVLAERADEVGLENVVNIPALMI"
     gene            complement(1077..1496)
                     /locus_tag="VFG22_03685"
     CDS             complement(1077..1496)
                     /locus_tag="VFG22_03685"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HET6415373.1"
                     /translation="MHRFPSKEWTAALRVALNNDRAYREVGKAWTFGAVAMIVRSDPA
                     NGIEQAAGIILDVHAGECRAAKFVEGTDDPPEAEFVIVASYARWKDVIEGKLDPIKGM
                     MQGKLKLTRGHLPTIIRFVEPSRLVVASARKVPTDFN"
     gene            1583..2341
                     /gene="pgeF"
                     /locus_tag="VFG22_03690"
     CDS             1583..2341
                     /gene="pgeF"
                     /locus_tag="VFG22_03690"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HET6415374.1"
                     /translation="MCALHSVVLGRHGFRHAFATRSGGVSVAPYDTLNLGFHLGDADE
                     SVHENRLRFARSLGLSRDRLFEQRQVHGTDVRVVSETDAADTFAELEGDALITRAEGY
                     AVAARTADCVPILIAHPPSGHVAAVHAGWRGAVAGILPRAIASLAHDPSELIVALGPH
                     IRVEAFEIGEEVAAEIEQAANGRPVVQRSAAKPHGDLAALIRLQLRDAGVPHEAVDDV
                     GGCTSRDAAQFFSHRREHGKTGRHLSAIVARASR"
     gene            2389..3228
                     /gene="accD"
                     /locus_tag="VFG22_03695"
     CDS             2389..3228
                     /gene="accD"
                     /locus_tag="VFG22_03695"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HET6415375.1"
                     /translation="MNEFAKTKASVDASEKRTLGEGLFRKCEACGDAVKKEDFERNLE
                     VCPACGFHFHLAAEQWIELLADEDTWTALDADLAAADPLSFVDSKPYLERVEAARAST
                     GVNDAILAGTARIDGRMVQLGVFVFRFMGGSMGSVVGEIVTRMMERGAEQRQPVVLLS
                     CSGGARMQEGSLSLMQMAKTVAARGKLRASGTPFISVVLNPTTGGVAASFALQGDVNI
                     AEPGALIGFAGPRVIEDTIRQKLPEGFQRAEFLLEHGMIDMISTRANIRDNIARMVEY
                     LSE"
     gene            3225..4496
                     /locus_tag="VFG22_03700"
     CDS             3225..4496
                     /locus_tag="VFG22_03700"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HET6415376.1"
                     /translation="MTRYQEALAWLYGLESRGIKLGLERIRSAAELRGHPEGALRYVH
                     IAGTNGKGSVATMLESVLRAAGYRTGQFASPHLQRYVERVRIGGRPISEREAAMRIEG
                     LRSDTRLPPLSFFEHTTLLAFEAFRDARCDIVLLEAGLGGRLDSTNIVTPDATVITNI
                     SREHERILGDTLWKIAREKAGILKPGVPGVVGTRVPSAKRSISARAKTVGAPLRWIDR
                     DFESEWSNGSLHVRVGDRQWDGLRLGLEGRHQADNAACVLATLNELSRAGFEISDADI
                     RSGLKKAKWPGRLEWHPGEPAFLFDAAHNASGCETLVRYLDELEPSGRVVLLFGAMRD
                     KNHQRMLAAFDGRVDHRVYAAPAVHRSEAPAVFAKIREGTVARSVRDAVSRAKRAAGS
                     DGLVVVAGSIFLLAEVRGLVKNVRTDPPIAM"
     gene            4543..5562
                     /gene="hemE"
                     /locus_tag="VFG22_03705"
     CDS             4543..5562
                     /gene="hemE"
                     /locus_tag="VFG22_03705"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HET6415377.1"
                     /translation="MSVFLQACRREPTSYTPVWIMRQAGRYQPEYRALREKVSFIELC
                     KSPDLACEVTVRAVEQLGVDAGIIFADILLILEPLQIGFEFTDDHGPKIMNPIRTAAQ
                     VDGIPERIDAAGSLSYVMDAIRTTRKELEVPLIGFAGAPFTLASYAIEGGGSKNYYEA
                     KKLMYGDEGLWNAFMSKLSSAIADYLIAQIDAGAQAVQLFDSWVGCLSPEDYRRYVLP
                     HSKAIFEKLPKNVPAIHFGTGNPALYPAMKEAGGDVIGIDWRISLNDAWSVLGEDVAL
                     QGNMDPAALLASTDVMRARASEVLESAAGRPGHIFNLGHGIMPEATPAQARALVDHVH
                     EASQS"
     gene            complement(5559..6248)
                     /locus_tag="VFG22_03710"
     CDS             complement(5559..6248)
                     /locus_tag="VFG22_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415378.1"
                     /translation="MKYIVLLIGALLMFGTTASAQDEAGTVTASEASAQPQEEKRSVY
                     YKKVQGLLWMEMFAGPSAYDPDKFGSVDINGTTANAPKANGPEFGGALGLGLGGFWLG
                     GFYRQANYNTYKLMKVGLDIQGIFRFIPWVHPMVRIDLFYGRTFNGDPFDTGLTNLNV
                     DGGGFTLGAGLRIPIIRWMSFAATFDWSMIGLAVRGNDSGGGSIKTGALGQQLAATFA
                     LTFHFIGVRGD"
     gene            complement(6248..6412)
                     /gene="yacG"
                     /locus_tag="VFG22_03715"
     CDS             complement(6248..6412)
                     /gene="yacG"
                     /locus_tag="VFG22_03715"
                     /inference="COORDINATES: protein motif:HMM:NF015821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HET6415379.1"
                     /translation="MGTARCPICKKQLSKRTKNFPFCSERCKLIDAGNWFEGNYRVET
                     KHDDFSWGEN"
     gene            6557..7144
                     /locus_tag="VFG22_03720"
     CDS             6557..7144
                     /locus_tag="VFG22_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415380.1"
                     /translation="MNRIPHLSYLDPLDAVWLTTARRIGFRVERSAEVYASTDGQGVM
                     IIGDPSTLDPDDCLAQMILHELCHSLVEGQESLAVPDFGLDNESERDLVREHACLRLQ
                     AWLTGEHGLRQALAPTTDFRIYYDTLPEDPLRDVDDPACASARQGAARSRQVPWAPHL
                     QEALKATATILRAAQRLGAKDPGADAPPIWSKLEE"
BASE COUNT         1329 a         2249 c         2224 g         1376 t
ORIGIN      
        1 gatcatcaga gccgggatgt tgaccacgtt ctccaggccg acttcgtccg ctctttcggc
       61 cagcacccgc gcatggttgg cgtggtgaaa ggccaggttc acagtgatcg gcggcgcgtt
      121 ggggcctgct ggctcgaggt cgttctgcgc atagaacggt ggatcgtccg cgtccatcag
      181 gtcgagcatg tcgacccgcg cccggtactc gagaatcgcc ggcgagtcga agtcatccac
      241 gtcggtgatg ccgtagaaat cgagcagcgc ctgcgccaag ccgagtgcat ccgcgagctc
      301 caagaggttc aatcggtatt ctgcaaagac cacggtcgtc cacttgccga tatcataggt
      361 cgcttgcgtg gcaatcgcga tcagcgcggt cactcgcgtc gactgtcgca gcaccggatc
      421 ctcgctgctc ggatcggcca tgtcatcgtt gaagccgatc caaaggctcg tgcccgcgcc
      481 agcggagccg cccatcagaa tgacgttgtc cgggtcgatg ttgagttgct ccgagtgata
      541 ccgaatgaac tgcagggcgc gcgtgctatc ggtcagcgac ttgatcaccc cgtccgggtc
      601 gggcgactgc aggagtcgat agtcgatcga cgcaaacgca acaccttccg cgagatacga
      661 catcacgaag tcctcgctct ccgacgtatc gcgggagcct ccagtgaagc cgccaccgtg
      721 gatgtaaatc agcagcggcg tcggctcgtc cgaagcgggc agatagatgt cgaacaggtt
      781 ctggtcgtac ggcccgtact ggatcgcttc gaagtactcc gcctcgaccg cgctcaggtc
      841 aaatgcggct tcggacgccg tgagctcgag cggaacgggc ggcgacgtgt tggtggagct
      901 ggagtcgctt ccgcaggaag ccgcgaatag cgccaagatg atgagagcag gtcgaaagag
      961 gctaagtgat ttcatggcgc gccgtgatag catcatcggc gcttcgcgca cgtttcggcg
     1021 agccctggcg ccgccccgaa gaacgcctga gaaccaactt tccccaggga ttttggtcag
     1081 ttgaagtccg tgggcacttt tcgagcgctg gccacgacca ggcgcgaagg ctccacgaag
     1141 cggatgatcg tgggcaggtg tccccgggtc agtttcagct ttccctgcat catccctttg
     1201 atggggtcga gctttccttc gatcacatct ttccagcgcg catacgacgc gacgatgacg
     1261 aactctgctt ccgggggatc atcggttcct tcgacgaact tcgcagctcg gcattccccc
     1321 gcgtgcacgt cgagaatgat tcccgccgcc tgttcgatgc cgtttgccgg gtcggaacgg
     1381 acgatcatgg ccaccgcgcc aaacgtccac gctttgccca cttcgcggta cgcgcgatcg
     1441 ttgttgagcg caactctcag cgctgcagtc cattctttcg agggaaaccg atgcatgagc
     1501 caactccaag cccggacttg aggaccgcct ccttagcccg cagcggaagc tcgcgcaatc
     1561 gcgtcgtgct acaaaccaca tcgtgtgcgc cttgcattcc gtcgttctcg gtcgccatgg
     1621 atttcgccat gcgtttgcta cccggtcggg tggggtgagc gttgctcctt acgataccct
     1681 caacctcggt ttccacttag gcgatgcgga cgaatcggtg catgagaatc gcttgcgctt
     1741 cgcacggagc ctcggtctct cgcgcgatcg cctattcgag cagcggcagg tgcacgggac
     1801 tgacgtgcga gtggtttccg aaaccgacgc agccgacacc ttcgccgagc tcgaaggcga
     1861 cgcactcatc acgcgtgccg aaggctacgc cgtcgcagca cgaaccgctg actgtgtacc
     1921 catcctgatc gcgcatcccc cgagcggaca cgtggccgcc gttcatgcag gctggcgcgg
     1981 agccgtggct gggatcttgc cgcgcgccat cgcatcgctt gctcacgatc cctcagagct
     2041 catcgttgcg ctcggcccgc acattcgcgt cgaagctttc gagatcggcg aagaggtcgc
     2101 ggcggaaatc gagcaggcgg cgaacggtcg tcccgtcgtt cagcgcagtg ccgcgaaacc
     2161 ccacggcgat ctcgctgcgc tgattcgatt gcaacttcga gacgcgggcg ttcctcacga
     2221 agccgtcgac gacgtgggcg gatgtacatc gagggatgcc gcgcagttct tttcgcaccg
     2281 gcgcgagcac ggaaaaaccg ggcggcactt gtcggccatc gtcgccagag cctctcgctg
     2341 acgagcttgc atctcgcttg cgattcggct tcatgctagc gaggggcagt gaacgagttc
     2401 gccaaaacca aggccagcgt ggacgcctcc gagaagagga cgctcggcga agggctcttc
     2461 cgcaagtgcg aagcctgcgg tgatgcggtg aagaaagaag acttcgagcg caatctcgag
     2521 gtctgtccgg cctgcggttt tcactttcat ttggcggcgg agcaatggat cgagctcctc
     2581 gccgacgaag atacctggac cgcgctcgac gcggacttgg cggccgccga ccctctgagc
     2641 ttcgtcgact cgaagcccta tctggagcgc gtggaagctg cgcgagcatc gaccggtgta
     2701 aacgacgcca tcctggcggg cacggctcga atcgatggac ggatggtgca actcggggtc
     2761 ttcgtattcc gattcatggg tgggtccatg gggtcggtgg tcggcgagat cgtcacacgc
     2821 atgatggaac gcggtgcgga gcagaggcag cccgtggtgc tcttgtcctg ttcgggtggc
     2881 gcacgaatgc aggagggctc gctctccctg atgcagatgg ccaagacggt ggccgcacgg
     2941 ggcaagcttc gcgcttcggg tacacctttc atcagtgtcg tgctcaatcc gacgaccggc
     3001 ggtgtcgcgg caagcttcgc tcttcagggc gacgtaaaca tcgcggagcc gggcgcactg
     3061 attggcttcg cagggccacg cgtcatcgag gacacgattc ggcagaagct cccggagggc
     3121 ttccagcgag cggagttcct tctcgagcac ggcatgatcg acatgatctc cacccgtgca
     3181 aacatacggg acaacatcgc gcgcatggtc gagtatctct cagagtgaca cgttaccagg
     3241 aggcgcttgc ttggctctac ggcctggagt ctcgcggaat caagcttggc ctcgaacgca
     3301 ttcgtagcgc cgcggagctg cgcggccatc cggagggcgc gctgcgctat gtccacatcg
     3361 cgggaaccaa cgggaagggc agcgtggcca cgatgctcga gtcggtgctc cgagccgcgg
     3421 gctatcggac cgggcagttc gcttcgccgc acctgcagcg atacgtagag cgcgtccgca
     3481 tcggcggcag gccgatttcg gagcgcgagg cggcaatgag gatcgagggt ctgcggtcgg
     3541 acacgcgcct gccgcccctt tcgttcttcg agcatacgac cctgctggca ttcgaggcct
     3601 ttcgcgacgc ccgctgcgac atcgtgcttc tggaagcggg actcggcggc cggctcgact
     3661 cgaccaacat cgtgacaccc gatgcaactg tgatcacgaa catcagccgc gaacacgagc
     3721 ggattctggg cgacacgctt tggaagatcg ctcgagagaa ggcgggcatc ttgaagccgg
     3781 gagttcccgg tgtcgtcggc acgcgcgttc caagcgcaaa gcgctccatc agcgcgcgtg
     3841 cgaagacggt tggagcaccc cttcgctgga tcgatcgcga cttcgaaagc gagtggagca
     3901 atggatcgct acatgtccgt gtgggggatc gccaatggga cggccttcga ctcggtctcg
     3961 agggaaggca ccaggcggac aacgctgcgt gcgtgctcgc gacgctgaac gagctctccc
     4021 gagccggctt cgagatttcg gatgcggaca ttcgttcggg cttgaagaag gccaagtggc
     4081 cgggccgtct cgagtggcac cccggcgagc ccgcgttttt gttcgacgcc gcgcacaacg
     4141 catcgggttg cgagacgctt gtgcgctacc tcgacgagct cgaaccgagc ggccgagtcg
     4201 tgctcttgtt cggagccatg cgggacaaga accaccagcg aatgcttgcg gcgttcgatg
     4261 gccgtgtaga ccaccgcgtc tatgccgccc cggcggttca tcggtctgaa gcccctgccg
     4321 tcttcgccaa gatccgcgag ggaaccgtgg cccgcagcgt gcgagatgca gtttcccggg
     4381 cgaaacgagc cgctggctcc gatggcttgg tcgtcgtcgc aggctcgatc ttcctgctcg
     4441 ctgaggttcg cggcctcgtg aagaacgtgc gcaccgaccc ccccatcgcg atgtaggcgt
     4501 tcgcttgctc gcgcggtgaa caagcgtgct atgccttcgc cgatgagtgt gtttctgcag
     4561 gcctgtcggc gtgagccgac ttcgtacacc ccggtttgga tcatgcggca ggcggggcgc
     4621 tatcaacccg agtatcgagc gctgcgtgag aaggtcagct tcatcgagct ctgcaagtcg
     4681 ccggacctcg cctgcgaggt gaccgttcgc gcggtcgagc aactaggagt ggatgccgga
     4741 atcatcttcg ccgacatcct cttgatcctc gagccgctcc agatcggttt cgagttcacc
     4801 gacgaccacg gtccgaagat catgaatccg attcgcaccg ctgcgcaggt cgacggcatt
     4861 cccgagcgca tcgatgcagc gggctcgctt tcgtacgtga tggacgcgat ccgaaccacg
     4921 cgtaaagagc tcgaagtccc gctcatcggt ttcgccggcg cgccgtttac cttggcctcg
     4981 tacgccatcg aaggtggtgg ttcgaagaac tactacgagg cgaagaagct gatgtacggc
     5041 gacgaaggcc tctggaacgc gttcatgtcc aagctctcga gcgccatcgc cgactatctc
     5101 atcgcgcaga tcgacgccgg cgcgcaggcg gtccagctct tcgacagctg ggtcggttgt
     5161 ctttcccccg aagactacag gcgttacgtc ttgccgcaca gcaaggccat cttcgagaag
     5221 ctcccgaaga atgttccggc gatccacttc ggcacgggca atcctgccct ctatccagcc
     5281 atgaaagaag cgggcggcga cgtgatcggc atcgactggc gtatctcgtt gaacgacgcc
     5341 tggtccgtcc tcggcgagga cgtcgccttg cagggaaaca tggatcctgc agcgctactc
     5401 gcgtccaccg atgtcatgcg cgcccgagcc agcgaggtcc tcgagagcgc agcagggcga
     5461 cccggccaca tcttcaatct cgggcacggc atcatgcccg aggcgacgcc ggcacaagcg
     5521 cgtgctctag tcgaccacgt gcacgaggcg agtcagagct agtccccgcg cacgccgatg
     5581 aagtgaaacg tgagggcgaa cgtagccgca agctgctggc ccaacgcacc tgtctttata
     5641 cttccgccgc cggaatcgtt gcctctgacc gccaatccga tcatcgacca atcgaaagtc
     5701 gccgcgaaag acatccaccg gatgatcgga atgcgtaagc ccgcgccgag cgtgaaccct
     5761 ccaccgtcca cgtttagatt ggtcaagccc gtgtcgaatg gatcgccatt gaacgttcga
     5821 ccgtagaaca gatcgatgcg aaccatcggg tgcacccagg ggatgaaccg gaagatcccc
     5881 tgaatgtcga gacccacctt catgagcttg tacgtgttgt agttggcttg ccggtagaat
     5941 cctccgagcc agaaccctcc aagaccgagc cccagcgcgc cgccaaattc ggggccattg
     6001 gccttcggcg cattcgccgt ggtgccattg atgtcgaccg agccgaactt gtcggggtcg
     6061 tatgcggatg gacccgcgaa catctccatc cagagcaagc cctgaacttt cttatagtac
     6121 acgctgcgct tctcttcttg tggctgcgcg ctggcctcgg acgcagtgac cgtgcccgcc
     6181 tcgtcctgcg ccgacgcggt cgtcccaaac atcaagagag ctccaatcaa caagactatg
     6241 tacttcatca gttctctccc catgagaagt cgtcgtgctt cgtctcgact cggtagttgc
     6301 cttcgaacca gttgccagcg tcgatcagct tgcatcgctc cgaacagaac gggaagttct
     6361 tggtccgttt ggatagctgt ttcttgcaaa ttggacagcg tgcggttccc actggtccga
     6421 acggtagcac ggaaaacccc acttctggga ggcctaccgg ttccgttgaa gcagcccgcc
     6481 gagatcggcc tccttcgcgc tctgtacgga cgagcgctag acgaaggctt ccgttgattt
     6541 cagagtttac gtcgaagtga atcgaatccc ccacctgagc tacctcgatc cgctcgatgc
     6601 cgtttggctc accacggccc gccgaattgg atttcgagtg gaacgctctg ccgaagtgta
     6661 tgcttcgacg gacgggcagg gcgtcatgat catcggcgat cccagcacgc tcgatcccga
     6721 cgattgcttg gctcagatga ttctgcacga gctctgtcat tccttggtcg agggacagga
     6781 gagcctcgcg gttccagact tcgggctcga caacgaaagc gagcgagatc tggttcgcga
     6841 acacgcctgt cttcgactgc aagcttggct taccggcgag cacggtcttc ggcaagcact
     6901 cgctccgacc accgattttc ggatctacta cgacacgctg cccgaggacc cgctccgcga
     6961 tgtcgacgat ccggcatgcg cgtcggcgag gcagggtgct gcgcgctcac ggcaagtgcc
     7021 ctgggctccg cacctgcaag aggcgctgaa ggcaacggcg acgatcctca gggcagccca
     7081 gcgcctcggc gccaaggacc cgggcgccga cgcgccaccc atttggtcga aattagaaga
     7141 gtaatttcgc tggcgctgac gctggcactg acgctggc
//
LOCUS       DASRZF010000084         3207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2812453, whole genome shotgun sequence.
ACCESSION   DASRZF010000084 DASRZF010000000
VERSION     DASRZF010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2812453"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VFG22_03725"
     CDS             complement(<1..429)
                     /locus_tag="VFG22_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415381.1"
                     /translation="MKHNLLTASLLLGLLIPAFASANPEPTAYDTRSIAMGLTGVTFL
                     DRPAALALNPANLEGIPKLGFTFNFTGLLVNQTAPVQGPNTSVSSGLGFGPLPSGFIA
                     GRIAPRVVFAGGIYIETGYGSSFSGVKCLDGAAVTPNPSSQ"
     gene            complement(552..1217)
                     /gene="msrA"
                     /locus_tag="VFG22_03730"
     CDS             complement(552..1217)
                     /gene="msrA"
                     /locus_tag="VFG22_03730"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HET6415382.1"
                     /translation="MFSFEKKVEMPSPERALPGRDAKMRVPAKHFVLGTPLEGPFPSD
                     TEMAMFGLGCFWGAERKFWESAGVHTTAVGYAGGYTPNPTYQEVCSGLTGHNEVVLVV
                     YKPAELVYEKLLQLFWESHDPTQGMRQGNDRGTQYRSGIYYFSDEQRELATASSLRYQ
                     SALADAGYARITTEIIAAPPFYYAEDYHQQYLAKNPAGYCGLGGTGVSCPTRVEPMVE
                     KDT"
     gene            1306..2070
                     /locus_tag="VFG22_03735"
     CDS             1306..2070
                     /locus_tag="VFG22_03735"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HET6415383.1"
                     /translation="METPQLSEIARRLRDSQSILFITGAGVSADSGLPTYRGIGGLYE
                     QEETDEGIPIEAALSGAMFRRNPALTWKYIHQIESSCRDAGPNRAHHAIAELERRFER
                     VWILTQNVDGLHHAAGSRNIIDIHGDVHRLVCTRCGHRWRVRSYLGLDIPPSCPECAS
                     LVRPEVVLFGEMLPMDRTRVLQTELTRGFDAVFSIGTTSVFPYIAQPVLDARRRGRLT
                     VEINPGTSEVSHVVDYRLRTGAAAAMDAVVQALDQP"
     gene            2083..2760
                     /locus_tag="VFG22_03740"
     CDS             2083..2760
                     /locus_tag="VFG22_03740"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6415384.1"
                     /translation="MGEQDYRYDASYEETVELSDGQRVHLRLMRPSDKAMLLEGFERL
                     SPDSRYARFMAPKSKLTESELRYLTEVDGVDHFAIGAIRRHLVSKDEGVGIARFVRLQ
                     EKPDTAEPAVTVSDDYQGKGLGSVLLQRLIEAAWERDVRWFVTELLAENAASRRMIEA
                     LSPEVKFGQSGDGALIATLPVPEPDHTPTAPGFFASSAVHKLLSYVARAKVSVRPRVI
                     RPPEPPI"
     gene            complement(2757..>3207)
                     /locus_tag="VFG22_03745"
     CDS             complement(2757..>3207)
                     /locus_tag="VFG22_03745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DJ-1 family glyoxalase III"
                     /protein_id="HET6415385.1"
                     /translation="GADPIRCSRGVVITPDVALASVVGEFDVIVLPGGAEGAKRLASS
                     LKVGELLRVQESAGRYVAAICAAPIALKAHGVFEHRQLTSHPSVKPELENWADYSEHS
                     VVADLNLITSRGPGTAFPFALRIVGTLTDAQRMVEVRAPMMFPVQVS"
BASE COUNT          604 a          981 c         1018 g          604 t
ORIGIN      
        1 ctgactcgag gggttcggtg tcacggccgc accgtcgagg cacttaaccc cgctgaacga
       61 cgagccatat cccgtctcga tgtagatacc acccgcaaac acgacgcggg gagcgattcg
      121 cccggcgatg aaacccgacg gcaagggtcc aaacccgagc ccagaagaga cactcgtgtt
      181 cggtccttgc accggcgccg tttgattcac gagcaggccg gtgaagttga acgtgaaccc
      241 gagcttcggg atgccctcga ggttggccgg gttgagggcg agggcggcag gccgatcgag
      301 gaacgtgacg cctgtaagac ccatcgcgat gctccgcgtg tcgtaggcgg tcggctccgg
      361 attcgcgctc gcaaacgctg gaatgagcag cccaagcaat aggcttgccg tcagtaaatt
      421 gtgcttcatt acggggctcc tccaccaacg tgcgccctgg gggggccgcc gcagcataca
      481 tggaagcacc gctgataggt caaccaaaaa aggctcgatc ggtgtacgga tatgcaggtg
      541 aaaacccgcg gctacgtgtc tttctcgacc atcggctcga cgcgggtcgg gcagctcact
      601 cccgtgccgc ccaatccaca gtagcctgct gggttcttcg cgaggtattg ctggtggtag
      661 tcctccgcgt agtaaaaagg cggggccgcg ataatctccg tcgtgatgcg agcgtatcct
      721 gcatcggcga gcgccgactg gtagcgcaat gacgatgcgg tcgccagttc gcgctgctcg
      781 tcactgaagt agtagatgcc agatcgatac tgagtcccac ggtcgtttcc ttgccgcatg
      841 ccctgcgtgg gatcgtgact ttcccagaac agctggagta gcttttcgta gacgagctct
      901 gcgggcttgt acacgacgag aacgacttcg ttgtggccgg tgaggcccga gcagacctct
      961 tggtacgtgg gattcggagt gtagcctccc gcatagccga cagcggtcgt gtgaaccccg
     1021 gcgctctccc agaactttcg ctcagcgccc cagaagcacc cgagcccgaa catcgccatt
     1081 tcagtgtcgc tcgggaacgg cccttcgaga ggcgtgccga ggacgaagtg tttcgcgggg
     1141 acgcgcatct tggcgtcccg tcccgggagg gctcgttcgg ggctgggcat ttcgaccttc
     1201 ttttcgaagc tgaacatgag ggatccatag tgatgggagg ccgcgtccgc tagggccgaa
     1261 ggcgcgaggc ttggcgttct gtggatattg ggataacgag attcgatgga aacgcctcag
     1321 ttatccgaga tcgcgaggcg cttgcgggac agccagagca tcctcttcat cacgggtgcg
     1381 ggtgtttcgg cagattcggg ccttccgacc taccggggca ttggaggatt gtacgagcaa
     1441 gaggaaaccg acgaagggat tccaatcgag gcggcccttt cgggcgcaat gttccgacgc
     1501 aaccctgcac tcacctggaa gtacatccat caaatcgaga gctcttgtcg agacgcgggt
     1561 ccaaatcgag cgcatcacgc gatcgcagag ctcgagcgac gcttcgagcg cgtctggata
     1621 ctcacccaga acgtggacgg cctgcatcat gcggcgggca gtcggaacat catcgacatt
     1681 catggagacg tccatcggct cgtctgcacg cgatgcggtc atcgctggcg cgtccgcagc
     1741 tacctcggtc tcgacatccc cccctcgtgt ccggaatgcg cctcattggt gagacccgag
     1801 gtcgtgctgt tcggcgagat gctccccatg gatcgcaccc gggtgctgca gacggagctc
     1861 acgcgcgggt tcgacgccgt cttctcgatc gggacgacga gcgtctttcc gtacatcgcg
     1921 cagcccgtgc tcgacgcgcg tcggcgagga agactcacgg tcgaaatcaa cccgggcacg
     1981 agtgaggtgt cccacgtcgt cgactatcgg ctacgaacgg gcgccgcggc ggcaatggat
     2041 gcggttgtgc aagccctcga tcaaccctag gctggctgtt gcatggggga gcaggactat
     2101 cgttacgacg cgtcctacga ggaaaccgtc gagctctcgg acggccaacg cgtccacctg
     2161 cggttgatgc gaccgtccga caaggcgatg ttgctcgagg gcttcgagcg cctctcgccg
     2221 gattcacggt atgctcgctt catggcgccg aaatccaagc tcaccgagtc tgagcttcgt
     2281 tacctcaccg aagtcgacgg tgtcgaccac ttcgcgatag gggcgatccg tcggcatttg
     2341 gtttcgaagg acgaaggcgt cggaatcgct cgattcgtac gactgcagga gaagccggac
     2401 acggcagagc cggcagtgac ggtctccgac gactatcagg gcaaagggct tggctcggtc
     2461 cttcttcaac gcctgatcga agcggcctgg gagcgcgatg ttcgctggtt cgtgaccgag
     2521 ttgctcgccg agaacgcggc ttcgaggcgg atgatcgagg ccctcagccc cgaagtgaaa
     2581 ttcggtcagt caggagacgg cgcgttgatc gcgacattgc cggttcccga gccggaccat
     2641 acgcccaccg cccccggttt ctttgcgagc tccgcagtac acaagctctt gtcatacgtg
     2701 gcccgagcca aggtttccgt gcggccccgc gtgatacggc cgcccgagcc gcccatttag
     2761 gagacctgta ctggaaacat catcggtgcg cgcacttcaa ccatacgctg tgcatcggtg
     2821 agggtgccga caatcctgag ggcgaagggg aacgcggtgc ccgggccgcg gctcgtgatc
     2881 aagttgaggt cggccaccac cgaatgctcg ctatagtcgg cccagttttc gagctctggc
     2941 ttcacactcg ggtgactggt gagttgacgg tgttcaaata ctccgtgcgc cttcagcgca
     3001 atcggtgcgg cgcaaatcgc cgcgacatat cggcccgcgc tctcctggac gcgcagcagc
     3061 tccccgactt tcaaagagga cgccagccgt ttcgcacctt cggcgccgcc cggcagcacg
     3121 atgacgtcga actcgccgac gacgctagca agggccacat cgggcgtgat gacgaccccg
     3181 cggctgcatc gaatcgggtc ggctccg
//
LOCUS       DASRZF010000085         6099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1438675, whole genome shotgun sequence.
ACCESSION   DASRZF010000085 DASRZF010000000
VERSION     DASRZF010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 6099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6099
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1438675"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..837)
                     /gene="rpsF"
                     /locus_tag="VFG22_03750"
     CDS             complement(154..837)
                     /gene="rpsF"
                     /locus_tag="VFG22_03750"
                     /inference="COORDINATES: protein motif:HMM:NF013419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HET6415386.1"
                     /translation="MASSAAASATRWAREYETIYILRPNVDNDAAEKIVDRLREVITR
                     LEGTLTKLDNWGKRKLAYPIQKNHRGIFVYLKYVGFNDLVAEIERNLRLLDEVVRFQT
                     VLIAEDIDPASIAVDPDEVKYLHVEEEEEESEDAELERARSLGMAPRASREAPRDSDD
                     DSDDDSDDDSDDDSDDDSDDDSDDDSDDSNDSKDKTKTSSVSKPKATADDDDETSNEA
                     ASDDEGEEG"
     gene            complement(923..1489)
                     /gene="pth"
                     /locus_tag="VFG22_03755"
     CDS             complement(923..1489)
                     /gene="pth"
                     /locus_tag="VFG22_03755"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HET6415387.1"
                     /translation="MGTHLIVGLGNPGPQYAGNRHNVGFMVIDELARRWGAPRSREKF
                     KGEFSKVAVGGEEIVLLKPMTYMNLSGESVQAAMQFFKVPLEQVVCIHDELDLEFGVV
                     RLKDGGGTAGHNGLRSMVAHCGPGFVRCRVGIGRPQHGRPEQYVLSDFNSLERAELGL
                     ALELAADMVDTSVRNGVRQAMNRHHDRS"
     gene            complement(1504..2163)
                     /locus_tag="VFG22_03760"
     CDS             complement(1504..2163)
                     /locus_tag="VFG22_03760"
                     /inference="COORDINATES: protein motif:HMM:NF004128.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HET6415388.1"
                     /translation="METTTLQAEVRDSRGKGPARRLRAEGKVPGVFYGPGIEPTPLTL
                     SPKELTKALRGERGRNVVFKLTVHGEPELAMVKDLTTDPVSQELLHIDLYRVFEDKEL
                     DVQVPFHTHGRAAGVVQGGVLNVTRRTLPVRTTPANIPVSIDVDVTHLNLRDSIAVED
                     VKLPPGVECTLQPKLTLAIILEPRKATAEEAAEDAAAAAAGAAATAASAAEADKAKAT
                     S"
     gene            complement(2179..3120)
                     /locus_tag="VFG22_03765"
     CDS             complement(2179..3120)
                     /locus_tag="VFG22_03765"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HET6415389.1"
                     /translation="MFKSTCVFSGNSNPALAEKIANYLELPMGRARVTRFSDGEVFAE
                     IKENVRGVDVYVIQSTCAPVNDNLMELLVMVDALKRASAGSITAVVPYYGYARQDRKA
                     APRTPITAKLVADLLSASGVDRVVSMDLHAGQIQGFFNKPFDHLYAMPVFLQHLRPRF
                     RTPPVFVSPDAGGVERTRAYAKRLDADLAIIDKRREQKNVSEVMNIIGDVDGRECLIL
                     DDMIDTAGTMTNAARALQEKGAKRVMAAATHPVLSGPALERIMDSPLEELIVTDTIPL
                     REEAKQTGRFRVLSVASLLGEAIKRIHHSDSVSSLFV"
     gene            3320..4135
                     /locus_tag="VFG22_03770"
     CDS             3320..4135
                     /locus_tag="VFG22_03770"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET6415390.1"
                     /translation="MTSSVTLLTEREHFVQQWQTALSEHEIVSRVVAPSDLANAVGGQ
                     VAVIVDGADLDADELLSTIGFVRASGALPVLHVERDGDMIEDIISEVCHGLVTRDEAD
                     LAPIAAAITRRANTRRGLRFEFVTVSPCSDDVLAVLGNGDASLHSRPIDASDDGSEIT
                     SITIDSTATKAVLQLASGAVCQLAVALVHGADTAHGHDDIAINGEKLGARLRELRLAA
                     GLTQAELARRTGIHRPNIARVEAGRHTPSLETLARIANAIGVSTTHVLVSQEP"
     gene            4132..4977
                     /locus_tag="VFG22_03775"
     CDS             4132..4977
                     /locus_tag="VFG22_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415391.1"
                     /translation="MSGAPEGKPIKDASTVIVLRETGGALETFMLCRHQQSGFMGGAH
                     VFPGGKVDPSDSDPGWRERIDRSAEDLSEQLGEADADVGLGLLVAAVRETFEEAGVLL
                     ASTSVDADLAEARQRLHDGAAFLDLAAELDLKIRSAALTPYARWITPKMESRRFDTRF
                     YIAVAPADQRARHDGTETTSAVWLRPARAIADMFEGRIKLAPPTVRTLQWLADFDDAD
                     SVIADALSRKPPLVRPEIVTGDGGWFLALPGDPAHPENEPVLPGATRMVFDNGAWREA
                     PLPVR"
     gene            5049..5624
                     /locus_tag="VFG22_03780"
     CDS             5049..5624
                     /locus_tag="VFG22_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6415392.1"
                     /translation="MTADARKPRDLFTASQIARFCQVDLKTIHNWADRDQIPHFRTPG
                     RHLRFRRPHVLDFLRKYGYPIPDELDAERPRVALLIENAGSKREIVAELRKAFDVVDY
                     TDPITGLLEIGEQPPDAIVLAATVGRFSGAEIIAALKRGSHTQHVRAVLLEDGGNGKE
                     AALHAGASAHVSGSDLRGLRDTLEALMGLRH"
BASE COUNT         1116 a         1927 c         1853 g         1203 t
ORIGIN      
        1 gcgatcggtg atgaagttcc gaagctgctg gggatctttg tagtcgaact tgaagtccgg
       61 aggtagcccc aagcccggcg ctttcttgcg tgcaccacgg cggcggccac cggcctcaac
      121 gtcgaaatcc tgtctaatgt cgtcgcccat ggctcagcct tcctcgccct cgtcgtcgga
      181 cgcagcttcg tttgatgtct catcgtcgtc atccgccgtc gccttcggct tggagacgga
      241 cgaggtcttt gttttgtcct tggaatcgtt ggaatcgtcg gaatcgtcgt ccgagtcgtc
      301 gtccgagtcg tcgtccgagt cgtcgtccga gtcgtcgtcg gaatcgtcgt ccgagtcgtc
      361 gtccgagtcg cggggggcct ctcgggacgc gcgcggcgcc atcccgaggc tccgagcacg
      421 ctccagctca gcgtcctcgg attcttcctc ttcttcttcg acatgaaggt acttgacctc
      481 gtcgggatcc acggcgatcg aagccgggtc gatgtcctcg gcgatcagga ccgtttggaa
      541 tcgcacgacc tcgtccagca agcgaaggtt gcgctcgatc tcggcaacga gatcattgaa
      601 cccgacgtac ttcagataaa cgaagatacc tcgatgattc ttctgaatcg ggtaagccaa
      661 cttgcgcttc ccccagttgt cgagcttcgt gagcgtaccc tctaggcggg tgatcacttc
      721 cctcagtcgg tcgacaattt tttcggcggc gtcgttgtcg acgtttggcc gcagaatgta
      781 aattgtctcg tattctcgag cccaccgcgt ggcggacgct gctgcgctgc ttgccatatc
      841 tcctcctggt ctggtcggcc ccagcgcatg gctggagcga gggaggccga ggcatagccg
      901 atcctgagtc gggtgccagt gctcaagacc tgtcgtggtg ccgattcatg gcctgacgga
      961 ccccgttgcg aacgctagtg tccaccatgt cggccgctag ctcgagcgcg agcccgagct
     1021 cagctctctc caagctattg aaatcgctaa gaacatactg ctccgggcgt ccatgctggg
     1081 gccgaccgat tccgacgcgg caacgtacga atcccgggcc gcagtgcgcc accatcgagc
     1141 gaagcccgtt gtgaccggcc gtgccgcctc cgtccttgag acgcaccacg ccaaactcga
     1201 ggtcgagctc gtcgtgaatg cacacgacct gctcgagcgg caccttgaaa aattgcatgg
     1261 cggcttgaac agactcgccg ctgaggttca tgtaggtcat cggtttgagc aggacgatct
     1321 cctcgccacc gaccgcaact ttgctgaact cgcccttgaa cttctcgcga gagcgaggcg
     1381 cgccccatcg ccgcgccaac tcgtcgatca ccatgaatcc aacgttgtgg cgatttccag
     1441 cgtactgggg cccgggattt cccaggccca cgatgaggtg cgtgcccacg agccagcagg
     1501 ctcctagctg gtggccttgg ccttatcggc ctcggctgcc gacgccgccg tagctgctgc
     1561 tcctgccgca gctgcagccg catcctcggc cgcttcctct gcggtggcct tgcgaggctc
     1621 gagaatgata gccagcgtga gcttgggctg cagggtgcat tccacgcccg gagggagctt
     1681 cacgtcttcg acggcaatgc tgtccctcag gttgagatga gtgacgtcca cgtcgatcga
     1741 taccgggatg tttgccgggg tcgtacgaac gggaagcgtg cgacgggtga cgttgaggac
     1801 gccaccttga accactccgg cggcccttcc atgggtgtgg aacggcacct ggacatcgag
     1861 ctccttgtcc tcgaacaccc gatacaaatc gatatggagc agctcctggc tcaccggatc
     1921 cgtggtgagg tccttgacca tcgccaactc gggctcgccg tgcacggtca gcttgaacac
     1981 tacgttcctt ccgcgctcgc ctcgaagtgc cttggttagt tctttggggg acagggtcag
     2041 cggcgtgggc tctatgcccg ggccgtaaaa cacgcccggg acctttcctt ctgcacgcag
     2101 ccgacgcgct ggcccctttc cgcggctatc gcggacctca gcctgcaacg tcgtagtttc
     2161 cattctcaaa tactccaatt atacgaacag cgagctcacc gagtcgctgt ggtgaatccg
     2221 tttgatggcc tctcctagca aacttgccac cgaaagcacg cggaaccgac cggtctgctt
     2281 ggcttcctcc cgaaggggta tcgtgtcggt cacgatgagc tcctccaggg gggaatccat
     2341 gatgcgttcg agcgccggac ccgacaagac cgggtgggta gccgccgcca tgacccgctt
     2401 ggcccccttc tcctgcaaag ccctggcagc gttcgtcatg gttccggccg tgtcgatcat
     2461 gtcgtccagg atcaagcact cgcgcccgtc cacatcgccg atgatgttca tgacttcact
     2521 cacgttcttc tgctcacgac gcttgtcgat gatggccaga tccgcatcga gacgcttcgc
     2581 gtaggcacgg gtgcgctcca cccctcctgc atcgggcgag acgaacaccg gtggggtcct
     2641 gaagcggggg cgcagatgct gcaagaacac gggcatcgcg taaagatggt caaaaggctt
     2701 gttgaaaaaa ccctgaatct gccctgcgtg caaatccatc gaaacgacgc gatcgacgcc
     2761 cgaggcgctc agcaggtccg cgaccaactt ggctgtgatc ggcgttcgag gcgcagcctt
     2821 tctgtcctgg cgcgcatatc cgtaataggg gacgactgcg gtgatcgagc cggcggacgc
     2881 acgcttgagc gcgtcgacca tgaccaatag ctccatcaaa ttgtcgttga ctggcgcgca
     2941 ggtggactgg atcacgtaga cgtcgacacc gcgaacgttt tccttgatct ccgcgaagac
     3001 ctcgccgtct gaaaaccgcg tgacccgcgc gcgacccatc ggcaattcca ggtagttggc
     3061 gatcttctcg gcgagagccg gatttgagtt tcctgaaaaa acgcaggtcg acttgaacat
     3121 cggtggtccc ctgaattgag caaagagctg ccacccggtg ggaaggcgtc cgggggtagc
     3181 aaagcgaccg tcgagtgtca accacgagtc gtttcggaaa gatcctcaac gagaggcgtt
     3241 ccgcagttcc tcgtgattca cggttgagct tcctgacgtg tgagtgtaca cctcatcgct
     3301 cccgttgccc ggaggcatga tgacgtcgag cgtaacattg ttgacggaac gcgagcactt
     3361 cgtgcagcaa tggcaaaccg ccctgagcga gcacgagatc gtttccagag tggttgcacc
     3421 aagcgacctc gccaacgcgg ttggcggaca ggttgcggtg attgtcgacg gagccgatct
     3481 cgatgctgac gagctccttt ctacgatcgg cttcgtgcgt gcttccggcg cattgcccgt
     3541 cctacatgtc gagcgcgatg gcgacatgat cgaagacatc attagcgagg tctgtcacgg
     3601 gctcgtgacc cgagatgaag cagacttggc ccccatcgcc gcggcgatca cgcggcgcgc
     3661 caatacgcga cgaggtcttc gcttcgagtt cgtcacggtg tccccttgca gtgatgacgt
     3721 gctcgcggtg ctcggcaatg gcgacgcgtc cctccattca cgtccgatcg atgcgagcga
     3781 cgatggatcc gagatcacga gcatcacgat cgattcgacc gcaaccaagg cggtacttca
     3841 gctcgcgagc ggtgcggtct gccagctcgc ggtcgcgctg gtgcatggcg cggacacggc
     3901 ccacgggcat gacgacattg caatcaatgg cgagaaattg ggcgcgcgtc ttcgtgagct
     3961 ccgtctcgcg gcgggtctca cgcaggccga gctcgcgcgt cgaaccggca ttcatcgccc
     4021 gaacatcgcg cgcgtagaag cggggcgaca cacgccctcg ctcgagacgc ttgcccggat
     4081 cgcaaatgcg atcggggttt cgacgaccca cgttctggtc tcacaagaac catgagcggc
     4141 gcgccggaag gcaagcctat caaggatgca tcgaccgtga tcgtactccg cgagacaggc
     4201 ggagcactcg agacgttcat gctctgcagg catcagcaga gcggcttcat ggggggcgca
     4261 cacgtgttcc ccggcgggaa agtggatccc agtgacagcg accccgggtg gcgcgaaaga
     4321 attgatcgct ccgcagaaga cctcagcgag caactcggcg aggccgatgc ggatgttggg
     4381 ctcggcctgc tggttgcggc cgtgcgggag acgttcgagg aagccggcgt cttgttggcg
     4441 agcacctccg tggatgctga cctcgccgaa gctcgccaac gccttcatga tggtgccgcg
     4501 ttcttggatc tcgcagcaga gctcgacctg aagatccgct ccgcggcgct cacgccctac
     4561 gcgcgctgga tcacccccaa gatggaaagc cgaagattcg acacgcgctt ctacatcgcc
     4621 gtcgcgcctg cagatcaacg cgcccgccac gacggaaccg aaacgacatc ggccgtctgg
     4681 cttcgcccgg ctcgcgcgat cgcagacatg ttcgaaggtc gaatcaagtt ggcaccgcca
     4741 acggttagaa cattgcagtg gttggccgac ttcgatgacg ctgactcagt gatcgcggac
     4801 gcgctgtccc ggaagccccc cctcgtccgc cccgagatcg tgaccggtga cggtggctgg
     4861 ttcttggcgc ttccggggga ccccgcgcac cccgagaacg aacccgtgct cccgggtgct
     4921 acccgtatgg tgtttgacaa cggcgcctgg cgggaagctc cattgcctgt tcgctaggct
     4981 acagattttc cagaatattc cggcttcttc ttgtctctcg gggtgcccgg cagctaccca
     5041 aggaggggat gacggccgat gcccgaaagc cgagggatct cttcacagcg tcgcaaatcg
     5101 cacgcttctg ccaggtggat ctgaagacga tccacaactg ggccgatcga gaccagatcc
     5161 cgcacttccg cacccctggc cggcatctcc gattccgtcg cccccacgtc ttggatttcc
     5221 tccgcaagta cggctacccg atcccagacg agttggacgc agagcgtccg cgcgtcgctt
     5281 tgctcatcga gaacgccggc tcgaagcgcg agatcgtcgc cgagctgcga aaggcattcg
     5341 acgtcgtcga ctacactgac ccgatcaccg gactccttga gatcggtgag caaccgcccg
     5401 acgcgatcgt cctcgctgcc acggtcggac gcttctctgg ggcagagatc atcgcggcgc
     5461 tgaagcgggg cagtcacacc cagcacgttc gcgcggtcct gttggaggac ggcggaaacg
     5521 ggaaggaagc cgccctccac gcgggcgcct cggcacacgt gagcggatcg gatctgcggg
     5581 gccttcgaga tactctcgaa gccctgatgg gccttcgaca ctgatctcgg acgcgggcgc
     5641 tggcagtggc gctgaactag cgagagcaaa gcctgctatt tgcatgctgg gtcagggcgc
     5701 acccgttgcg cccaccaccc cgacagacgc agcacttgcg gtcatggttt tgtaataccg
     5761 ccaagaatcc tacaaatccg cgctggcaca ccaaatgcat cagcatcgct cgccttgttg
     5821 gcgctccggt ggggagggct gaaatcggga gtttgtcatg cgctacttgt tcggactcct
     5881 ttgtgtctgc gcgtcggtcg agactttgcc tctgagcgcg agcgcacaag aggaggaggt
     5941 ttcttcttag ccagcacccg aaaagcccgc gctgcagctc aagctagact ctgccggtgt
     6001 tgaggctgcc ccctgtgcca agcgcgcccc ggacaggcga cggatatacg ctcgaacaga
     6061 tggagcttcg ggtgaagcga gcgaagatcg ggctctgga
//
LOCUS       DASRZF010000086         4720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1093889, whole genome shotgun sequence.
ACCESSION   DASRZF010000086 DASRZF010000000
VERSION     DASRZF010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093889"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VFG22_03785"
     CDS             complement(<1..263)
                     /locus_tag="VFG22_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET6415393.1"
                     /translation="MAKEKFVRNKPHINVGTIGHIDHGKTTLTAAIMKVMSDKHNGEV
                     KSYKDIAKGGTERDDTKTVTIATSHVEYQTGNRHYAHVDCPGHA"
     gene            complement(325..396)
                     /locus_tag="VFG22_03790"
     tRNA            complement(325..396)
                     /locus_tag="VFG22_03790"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(362..364),aa:Thr,seq:ggt)
     gene            complement(436..511)
                     /locus_tag="VFG22_03795"
     tRNA            complement(436..511)
                     /locus_tag="VFG22_03795"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(476..478),aa:Gly,seq:tcc)
     gene            complement(533..618)
                     /locus_tag="VFG22_03800"
     tRNA            complement(533..618)
                     /locus_tag="VFG22_03800"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(582..584),aa:Tyr,seq:gta)
     gene            complement(644..716)
                     /locus_tag="VFG22_03805"
     tRNA            complement(644..716)
                     /locus_tag="VFG22_03805"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(681..683),aa:Thr,seq:tgt)
     gene            complement(772..1179)
                     /locus_tag="VFG22_03810"
     CDS             complement(772..1179)
                     /locus_tag="VFG22_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415394.1"
                     /translation="MCAIGVAACVSGLLLGCGDSGETEADRIGVGAQCDAAAECESAD
                     ENITLECLTQFTGGYCGLEGCEGDADCPEGSACVTHDDGGGAANYCFRVCVNKPECNR
                     NRDEANWSNCSANITFVDEPQDRKACVPPSSGI"
     gene            complement(1206..2444)
                     /locus_tag="VFG22_03815"
     CDS             complement(1206..2444)
                     /locus_tag="VFG22_03815"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET6415395.1"
                     /translation="MVVSLHSEPPSLRPPPEDAGSQARAKPRVSVIPFSASLLPTGSP
                     FGWNARDRALYVQFLTVLLMVIVAFSFAVLDGWPFLLERPDDGAALRTTTALAVGALL
                     FWIGFYACTWRWYVRRKAKFSWLLFATIAAHIITGTAVCYAIGPYTAASGLIMVAALL
                     LGMVLLERRSFWIAVIGWFTSMFAMHFASKAGLLPFLFLLDPSFAHSHLARVAAERAV
                     AAALIYIPLAAIMDHLIGVWRQDELSLYEAVQFDSLTGAYTRGFAMAVMQRALTKARA
                     DSNPLAVLMLDLDHFKRINDAHGHARGDLVLAQTTHAVQSTLRAGDIVGRFGGEEFVI
                     LLPGADLAQAVGAAERCRAAVAKTEISEAPHLSVTASVGVAAFPDHGEDLDALLKASD
                     RAMYAAKAHGRNRIETAEAG"
     gene            complement(2485..2853)
                     /locus_tag="VFG22_03820"
     CDS             complement(2485..2853)
                     /locus_tag="VFG22_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6415396.1"
                     /translation="MAPGAHGETLVPVALYAALVCDEEVLPAFVTSISEYGMVFDSLA
                     IFANRSCDRLQIELTLPGEPELLWIGAEVVRDTRGALFNDSAIRFLAMANAHWRSIRR
                     WVRVRELVLATRPIGLHDAA"
     gene            3010..3627
                     /locus_tag="VFG22_03825"
     CDS             3010..3627
                     /locus_tag="VFG22_03825"
                     /inference="COORDINATES: protein motif:HMM:NF019958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6415397.1"
                     /translation="MAVHSTANKRRGSVPDKRARILKAAIKVFAKKGFYATRVSEIAK
                     AAGVADGTIYLYFKNKDDVLITIFEDGLKQLLEILQSVAESEESFENRVTRIIELQLG
                     LLEDQRDLAEVITVNLRQSSRLMKQYAAPLFMQYIDVIAGVIREGQEAGAFRKDLNPR
                     VVARSLFGALDAILLTWALGDSDPVALRKAAAHCASLILEGLRQR"
     gene            complement(3660..3950)
                     /locus_tag="VFG22_03830"
     CDS             complement(3660..3950)
                     /locus_tag="VFG22_03830"
                     /inference="COORDINATES: protein motif:HMM:NF012280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Kazal-type serine protease inhibitor
                     domain-containing protein"
                     /protein_id="HET6415398.1"
                     /translation="MSIRVYALFGLLAFALGCGDSNSIDSACRSNADCAETEICATGI
                     CEGGIGICNARPTSCPDTDSPVCGCDGVTYQNTCFATMAGVRIAITLPCTEM"
     gene            complement(4064..>4720)
                     /locus_tag="VFG22_03835"
     CDS             complement(4064..>4720)
                     /locus_tag="VFG22_03835"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6415399.1"
                     /translation="LVAVRELALEVKTRFASLDVLVNNAGVWHRERTLSEDGYEDTFA
                     VNHLAHFLLTTELKDLLTAGSESRVVNVSSSMHVRPKSLNWDDLMFRNGWRGFWVYGH
                     SKLANVLFSNELARRWKDLGVTSNALNPGNVRSRITRNNVLLNALHRSPIARMLIMSE
                     AEGAKTSLHVATSPELRGVTGRYFDRVRPAKPSKASEDRNAAERLWDVSKELIHSVIG
                     "
BASE COUNT          959 a         1451 c         1426 g          884 t
ORIGIN      
        1 gcgtgcccgg ggcaatcgac gtgcgcgtag tgacggttgc ccgtctggta ctccacgtgg
       61 ctcgtcgcga tcgtcaccgt cttggtgtcg tcgcgctccg tcccgccctt cgcgatgtct
      121 ttgtagctct tgacctcgcc gttgtgcttg tcggacatca ccttcatgat cgccgccgtc
      181 agcgtcgtct tgccatggtc gatgtgacca atcgtgccga cgttgatgtg tggcttgttt
      241 cggacaaact tttctttagc catcgttcgc ttcctacttc gtcattcttc tgagtgagct
      301 tgcttgaact cactaaattt ggggagccca ggatgagact tgaactcacg acctccccct
      361 taccaagggg gtgctctacc actgagctac ctgggcatag tctgaatcgc gtctcgtttc
      421 acctgcactg cgggttggag cgggcgaccg ggctcgaacc ggcgacattc agtttggaaa
      481 actgaggctc taccaactga gctacacccg cgtaatctgt cttcttatct cctggtggag
      541 ggtggtgggt tcgaaccacc gtaggcataa gccggcagat ttacagtctg ctccctttga
      601 ccgctcgggc aaccctccat ggtcattccg ttttgtcgtg tagagctggc gatgagactt
      661 gaactcataa ccgccggttt acaaaaccgg tgctctacca attgagctac gccagcatga
      721 cggcggcggc tgtactacgg tccgaccctc gggaagtcaa gggaatcggg gctagatacc
      781 ggaggatggt ggcacgcagg cctttcggtc ctgcggctcg tcgacgaagg tgatgttcgc
      841 cgaacaattc gaccaattcg cttcatcacg gttgcgattg cattccggct tgttcacgca
      901 gaccctgaaa cagtagttcg ctgcgccacc gccgtcgtca tgggtgacgc acgctgaccc
      961 ttctgggcaa tccgcgtcgc cctcgcagcc ttcgagcccg cagtacccgc ccgtgaactg
     1021 ggtgaggcac tcgagcgtga tgttttcatc tgcggattcg cactccgcgg ccgcgtcgca
     1081 ctgcgcgccc accccaatgc ggtcggcctc cgtttccccg gaatctccgc accccagcag
     1141 gagccccgaa acacaagccg caacgccaat agcacacaca aacctacaca taactacccc
     1201 ctgcctcagc ccgcctctgc cgtctcgatg cgattgcgcc cgtgggcctt ggctgcgtac
     1261 atcgcccgat cggacgcttt gagcaacgcg tcgaggtcct ctccatgatc tggaaacgcc
     1321 gcgactccga cgctggccgt gaccgacagg tgcggcgcct cggaaatctc cgttttcgcc
     1381 accgcagcac ggcaacgctc ggcggctcca accgcctgcg ccaagtccgc cccgggaaga
     1441 aggatgacga attcctctcc tccgaatcgc ccaacgatgt cgccagcgcg cagcgtggac
     1501 tgaaccgcgt gcgtcgtttg cgcgagcacg aggtcacctc gcgcgtggcc atgggcatca
     1561 ttgatccgtt tgaagtggtc gaggtcgagc atgagcactg ccaatggatt ggaatcggcg
     1621 cgtgctttcg tgagcgctcg ctgcatgacg gccatggcaa agccgcgcgt gtaggcacca
     1681 gtcagggaat caaactggac tgcttcgtaa agactcagtt cgtcttggcg ccaaacgccg
     1741 atcagatgat ccatgatcgc cgcaagcgga atgtagatca gcgcggcggc tacggcgcgt
     1801 tccgcggcga cccttgcgag gtgcgagtgc gcgaaggacg ggtccaacaa gaacaggaac
     1861 ggcagcaacc cggccttgct cgcgaagtgc atcgcgaaca tggacgtgaa ccaaccgatg
     1921 accgcgatcc aaaacgagcg ccgctccagc aagaccatgc ccaggagcag agcggcgacc
     1981 atgatgagtc cgctcgcggc cgtgtagggt ccgatcgcgt aacacacggc cgtgccggtg
     2041 atgatgtgcg cggcgatcgt cgcgaagagc agccaggaga acttcgcttt gcgcctcacg
     2101 taccagcgcc aggtgcaagc gtagaagccg atccagaaca ggagcgcgcc gaccgcaagc
     2161 gccgtagtcg ttcgaagggc cgcaccgtcg tcgggtcgtt cgaggaggaa cggccagccg
     2221 tcgagaacgg caaacgaaaa agcgacgatc accatcagaa gcaccgtgag aaactgcacg
     2281 tagagggcgc gatcgcgggc gttccatccg aagggcgagc ccgtgggcag cagtgatgcg
     2341 ctgaagggga tcaccgacac acgcggcttg gcccgcgcct gacttcctgc atcttccggc
     2401 ggcggccgaa ggctgggcgg ctcgctgtgt agactgacga ccatgtcccc ctttcaggct
     2461 agcaagtcgg agggcttgct gtcctcaagc tgcgtcgtga aggccgatcg gacgtgtcgc
     2521 caagacgagc tcccgaacac gaacccagcg acgaatcgag cgccaatgcg cgttggccat
     2581 cgccaagaaa cgaatcgcgc tatcgttgaa gagagcgccc cgcgtatccc gaaccacttc
     2641 ggcgccgatc cagagcagct ctggttcgcc cgggagggtg agctcgatct gaagccggtc
     2701 gcagctccgg ttggcaaaga tcgccaatga atcgaaaacc atcccgtact cgctgatgct
     2761 agtcacgaaa gcgggaagca cttcctcgtc gcatacgagc gcggcgtaga gggccacggg
     2821 aacgagcgtc tcaccatgag cgccgggagc catgagctcg aagctacggg gagcaggttc
     2881 gctgggcaag aaactcaccg caacggcaac gcgccatatc gttctgattc taaggtgata
     2941 ggcgccgccc ggcgcttgcc cacgaacctg gtttgacgga aagccagcgg ccgtgttacc
     3001 ggcagctgaa tggccgttca ttcaacagcc aacaagcgtc gcggctccgt cccggacaag
     3061 cgggctcgga tcctgaaggc ggcgatcaag gtcttcgcca agaagggctt ctacgcgact
     3121 cgtgtgagcg aaatcgcgaa ggcggcggga gtcgccgatg gaacgattta tctctacttc
     3181 aagaacaagg acgatgtcct catcacgatc ttcgaagacg ggctgaagca gctcctcgag
     3241 attctgcaaa gtgtggccga gtccgaggag agcttcgaga accgagtcac gcggatcatc
     3301 gagctgcagc tcggactgct agaggaccag cgtgatctag ccgaggtgat cacggtcaat
     3361 ctgcgacagt cgtctcgcct catgaagcag tatgcggccc ctttgttcat gcaatacatc
     3421 gatgtcatcg ccggcgtcat tcgggaagga caggaagcag gggcgttccg caaagatctg
     3481 aacccgcggg tcgtggctcg cagccttttc ggtgcactcg atgcgatatt gctcacctgg
     3541 gctctcggag acagcgaccc cgtagcgttg cgaaaagccg cggcgcattg cgccagcctc
     3601 atcctcgaag gtctccgcca gcgctgacac gatcggcgca agggaactga gggtgcagct
     3661 cacatctccg tgcaagggag tgtgatagcg atacggacac ccgccatcgt cgcaaagcag
     3721 gtgttctggt acgtcacgcc gtcgcaacca cagacggggc tatccgtgtc gggacagctc
     3781 gtaggccgcg cattgcagat cccaatcccg ccttcgcaga tcccggttgc gcaaatctcc
     3841 gtctctgcac agtcggcgtt cgagcggcag gcagaatcga tcgaattgct gtcgccgcaa
     3901 ccaagagcga acgcgagcaa accgaagaga gcatagacgc gaatggacat cgatcaaaac
     3961 gctagcccag gacctgagct cgtaccagct gggtgtcggt gcattccggc aacatcgaga
     4021 tggcagtttt ggaacgagtc gatcttcgag cgaagcggga cgactaccct atgaccgaat
     4081 ggatcagctc tttgctgacg tcccagaggc gctctgcagc gtttcgatct tcggatgcct
     4141 tgctcggctt cgcgggtcgc actctgtcga agtagcggcc cgtcacgccc ctgagctctg
     4201 gcgaagtcgc aacgtgaagc gaagtcttgg cgccctcggc ctccgacatg atgagcatgc
     4261 gcgcgatcgg cgagcggtgt agcgcgttga gcaggacgtt gttgcgagtg atgcgactgc
     4321 gcacgttgcc agggttcaag gcgttggagg tgacgccgag atccttccag cggcgggcca
     4381 gctcgttgga aaagagcacg ttggcgagct tggagtgacc gtagacccag aatcctcgcc
     4441 aaccgttgcg aaacatcaaa tcgtcccagt tgagtgactt gggtcgcaca tgcatcgacg
     4501 acgagacgtt caccacacgg gactccgaac ctgccgtcag aaggtccttg agctcggtgg
     4561 tcagcaggaa gtgggcgaga tggttgaccg cgaaagtatc ctcgtatcca tcctcgctca
     4621 gcgtgcgttc ccggtgccat accccagcgt tgttcaccag cacgtcgagc gacgcgaagc
     4681 gggtcttgac ctctagcgcc aactcacgca ccgcgacgag
//
LOCUS       DASRZF010000087         2736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_406447, whole genome shotgun sequence.
ACCESSION   DASRZF010000087 DASRZF010000000
VERSION     DASRZF010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406447"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VFG22_03840"
     CDS             <1..600
                     /locus_tag="VFG22_03840"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6415400.1"
                     /translation="VLIAERGGVAYCVPMSEVSVNAETSVDRTRELGRVPFEATARNQ
                     MREDVDAMGLFAMGRERATLGTAAQLIGLSRRMLDMAVQYAKDREQFGKAIGAQQAVK
                     HRLANALIEQEFARPMVYRAAWSMATSAQDAEIHVSLAKIYAGRAARFVAKEALQVHG
                     AIGYTIECDLHMWMKRAWALAAAHGDAAYHRERVGRHIL"
     gene            677..1831
                     /locus_tag="VFG22_03845"
     CDS             677..1831
                     /locus_tag="VFG22_03845"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF005865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HET6415401.1"
                     /translation="MPEAYIVEAVRSPVGRKKGGLSKVHSADLGAHAIKGLIERAGID
                     PGIVDDVVFGCVDSVATQAGDIARTCWLAAGYPEHVPGVTIDRQCGSSQQAVHFAAQG
                     VMSGTQDVVVAGGVQNMSMIPIGASMIAGKDLGIRDPFSGSVGWTERYGDKPVNQFYG
                     AEMIAKDWDLSREELERFALESHRRALQAQKEGRFDREIIPFGDVTADEGPRADTTLE
                     KMAALPVLMDGGRLTAAVASQISDGSAALLVVSEKALKEYKLTPRARIHHLSVRGEDP
                     IRMLSAPIPATKYAMKKSGMSLDEIDLVEINEAFAPVVCAWAKELEVDLAKVNVNGGA
                     IALGHPLGATGAKLMTTLLHELERTGGRYGLQTMCEGGGQANVTIIERLG"
     gene            1960..2328
                     /locus_tag="VFG22_03850"
     CDS             1960..2328
                     /locus_tag="VFG22_03850"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6415402.1"
                     /translation="MSSKLPNHGSNRPPPIRKHSEVRPSVQPDDRRHTERWASDARIE
                     ILSPVRAEASAIDVSVTGLQVALNGWLSTGTLCDLRITTHTGRVIFKRARVVWARRVG
                     EQCVAGLYVVGSIVPPAETE"
     gene            2492..>2736
                     /locus_tag="VFG22_03855"
     CDS             2492..>2736
                     /locus_tag="VFG22_03855"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HET6415403.1"
                     /translation="MAKVANTAEYFDTLADRFLADQAKGVNAQIQYTLSGEGGGEWTI
                     TIADGQFQGVTTGGVEKPTLNVMMDAAKFVEMANGDL"
BASE COUNT          543 a          813 c          884 g          496 t
ORIGIN      
        1 gtcttgatcg ccgagcgggg cggggtcgcg tactgcgtgc cgatgtcgga agtctccgtg
       61 aacgcggaaa cttcggtcga ccgcacccgc gagcttggac gcgtgccgtt cgaggcaact
      121 gccaggaacc aaatgcgcga agacgtcgat gcgatgggcc tcttcgcgat gggccgcgag
      181 cgggcgactc tcggcacggc agcacagctc ataggtctct cgcgacgcat gctcgacatg
      241 gcggtgcaat acgcaaaaga ccgcgagcaa ttcggcaaag cgatcggcgc ccagcaagcg
      301 gtaaaacacc gtctagccaa cgcgttaatc gagcaggagt tcgcgcggcc catggtctac
      361 cgcgcggcct ggtccatggc gacttcggcg caggatgccg aaatccatgt ttcccttgcc
      421 aagatctatg caggcagggc tgcaagattc gtggccaagg aagcgcttca ggtccatggc
      481 gccatcggct acacgatcga gtgcgacctt cacatgtgga tgaaaagggc gtgggcgctt
      541 gccgcggctc acggggacgc cgcctaccat cgcgagcgcg tgggccggca catcctttga
      601 gtgcttcgga gcttttgcag tggcgccttg cgggcgacga ctgctccccc tagaaatacg
      661 aaatagcgag gttcagatgc cggaagcata catcgtcgaa gcagttcgtt cgcccgtcgg
      721 aagaaaaaag ggcgggctca gcaaagttca ctccgccgac ctcggcgcac atgccatcaa
      781 ggggctgatc gagcgcgccg gcatcgatcc gggcatcgtc gacgacgtcg tgtttggctg
      841 cgtcgattcg gttgcaacgc aggccgggga catcgcgcgc acatgctggt tggctgcagg
      901 ctaccccgag cacgttcccg gcgtcaccat cgaccgtcag tgtggatcct cccagcaggc
      961 ggtgcacttc gcggcgcagg gcgtcatgag cggaacccag gacgtggtcg tggctggggg
     1021 cgttcagaac atgagcatga tcccgatcgg cgcgtcgatg attgccggca aagacctcgg
     1081 tatccgagat ccattttctg ggtcggtggg ttggaccgaa cgctacggcg ataaaccggt
     1141 caaccagttt tacggcgccg aaatgatcgc caaggactgg gacctctccc gcgaagagct
     1201 cgagaggttt gctctcgaga gtcatcggag agcgctccaa gctcagaaag agggccgttt
     1261 cgatcgcgag atcatcccgt tcggcgacgt caccgcggac gaaggcccgc gtgccgatac
     1321 gacgctcgaa aagatggctg cccttccggt cctgatggac ggtggccggc tcacggcggc
     1381 agtcgccagc cagatctccg atggctccgc tgcgctgctg gtggtctcgg agaaggcgct
     1441 caaggagtac aagctcacgc cgcgcgctcg catccaccac ttgagcgttc gaggtgagga
     1501 tcccatccgc atgctctcgg cgccgattcc cgcaaccaag tatgcgatga agaaatcggg
     1561 catgagcctc gacgagatcg atctcgtcga gatcaacgag gcgtttgcgc ccgtggtctg
     1621 cgcttgggcg aaggagctcg aggtcgacct cgcgaaggtg aacgtgaatg gtggcgccat
     1681 tgccctgggt catccgctcg gtgcaacagg ggccaagctc atgacgactc tgcttcacga
     1741 gctcgagcga accggtggcc gttacggctt gcagaccatg tgtgaaggtg gcggacaggc
     1801 gaacgtcacc atcatcgagc gcctcggcta agtccttcat cggggactga ggcgatcgcc
     1861 tggcgagcga atcagccctg aaatgtgaca ttccgaacag tagatggcga ctttgtcgac
     1921 ttcgttttcc ccggtcggca tgatgaagct tcgggacaca tgagctccaa gctcccaaat
     1981 cacggctcga atcgaccgcc tccgatcagg aagcactcgg aggttcggcc ttcggtccag
     2041 cccgatgatc ggcgccacac cgaacgctgg gcctcggacg ctcgaatcga gatcctctca
     2101 cccgttcgcg ccgaggcatc tgcgatcgat gtgagcgtca ctggactcca agtggcgctg
     2161 aacggctggc tctcgactgg gactctctgc gatctgcgca tcacgacgca cacaggccga
     2221 gtgatcttca agcgagctcg agttgtttgg gcaaggcggg tcggcgagca atgcgtggcc
     2281 gggctctacg tggtcggctc gatcgtcccg ccagccgaga ccgaatagcg tcgtgccaga
     2341 cgtgttcgtc tgaaagcctg gccttcgaga ccgggtttct agggcttttt tgggggactc
     2401 ggccacaggc ggcttgacgg atcggtccgc gcaaaataga acatgtttca ctcgggatga
     2461 cgctttttcg acccggaacc gggaggtgaa catggccaaa gtagccaaca cagcagagta
     2521 tttcgacaca cttgccgatc gctttctggc cgaccaggcc aagggggtga acgcccagat
     2581 tcagtacacc ctgagcgggg aaggcggtgg cgagtggacc atcacgattg cggatgggca
     2641 gtttcaaggc gtcaccaccg gcggcgtcga gaagccgacg ctcaacgtga tgatggacgc
     2701 cgcgaagttc gtcgagatgg ccaatggcga cctcga
//
LOCUS       DASRZF010000088         9044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_750344, whole genome shotgun sequence.
ACCESSION   DASRZF010000088 DASRZF010000000
VERSION     DASRZF010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 9044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9044
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750344"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VFG22_03860"
     CDS             <1..280
                     /locus_tag="VFG22_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415404.1"
                     /translation="PVRTFLRAMEAFERYVDPDRCVKLLKETLAMRPDLVRAQADLVL
                     VSESIDARYAELQALKKISPTHIVVRLSGRMIEEEYETAKELQGAFKP"
     gene            complement(527..832)
                     /locus_tag="VFG22_03865"
     CDS             complement(527..832)
                     /locus_tag="VFG22_03865"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET6415405.1"
                     /translation="MGVRRAALALVALLALGCAKNNTIAPEPARLRVITSPATASVYI
                     DGHYFGRGTVLSQEPKALAPGVHLMTVEAEDHFPHDMELDLPKGESTITIELRPIPP"
     gene            complement(823..2367)
                     /gene="guaA"
                     /locus_tag="VFG22_03870"
     CDS             complement(823..2367)
                     /gene="guaA"
                     /locus_tag="VFG22_03870"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HET6415406.1"
                     /translation="MIVDGVLILDFGSQYTQLIARRIREQHVYCEIQPCTYSLAEVKD
                     IAPRAIILSGGPASVFADGSPTVDPGVFDLGVPVLGICYGMQLTSHLLGGKVQRADER
                     EYGPARLEVLKPEGLLHGFQAGSHVDVWMSHGDRVESLPPGFETIARTSNSPFAAVAN
                     LERNIFGVQFHPEVAHTPRGVEMLRAFLFEVAGCAANWTPGSFVEQSVDSIRRQVGKR
                     AAVCGLSGGVDSSVAAMLVSKAIGDRLTCIFVDNGLLRKDEAESVVHTYRDHFGLKLI
                     HVDAADEFLDQLAGVSDPEKKRKIIGRVFIDVFQREAEKLDDVGYLVQGTLYPDVIES
                     LSFKGPSAVIKSHHNVGGLPDTLHLELIEPLRLLFKDEVREVGEAMGMPRDVLWRHPF
                     PGPGLAVRCPGAIEREKLDILREADAIFDEELRAADLYDKIWQAFCVILPVRTVGVMG
                     DERTYDYVIAVRAVDSRDGMTADWSRIPHEVLARISTRIINEVRGCNRVCYDISSKPP
                     STIEWE"
     gene            complement(2834..4294)
                     /gene="guaB"
                     /locus_tag="VFG22_03875"
     CDS             complement(2834..4294)
                     /gene="guaB"
                     /locus_tag="VFG22_03875"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HET6415407.1"
                     /translation="MTDSILREALTFDDVLLLPGYADFLPANADVSTRFTRGLELRIP
                     FVSSAMDTVTEWRTAVTMAREGGIGILHKNMSPELQALEVTKVKRAETGMIVDPVTVG
                     PGESLHDALELMRRHEISGLPVVEGTKPVGILTSRDIRFEQNLNQKVAQLMTKELVTV
                     PPGVEQDKARELLHKHRIEKLLVVGDDGDLVGLITIKDLLQADRYPDSIKDSLGRLCV
                     GAALGVGPDRENRTEALVRAGVDVLIIDTAHGHTKGVIDAVRETKSTYPEVQVVAGNV
                     ATAAGTKALIDAGADAVKVGMGPGSICTTRVVAGIGVPQVTAVMDCARVGREHGVPII
                     ADGGVKYSGDAVKAIAAGAESVMIGSLFAGTDEAPGELVLYQGRTYKSYRGMGSLGAM
                     REGSSDRYAQSDISDVGKLVPEGIEGRVPYRGALSTVVYQLVGGLRAGMGYTGNPSIA
                     ELQKNAEFVRISAAGLHESHVHDVMVTKEAPNYRVS"
     gene            complement(4354..4662)
                     /locus_tag="VFG22_03880"
     CDS             complement(4354..4662)
                     /locus_tag="VFG22_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415408.1"
                     /translation="MKRREGTGVESVFEEDEVNAVLRDTFYGDKGGRRGRAKTIKPKA
                     KKPDHYDVICISLYTEDLARLDKMVAKLKKQGHRRISRSALIRFALDQVDIDEFPRAY
                     "
     gene            complement(4671..5744)
                     /locus_tag="VFG22_03885"
     CDS             complement(4671..5744)
                     /locus_tag="VFG22_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HET6415409.1"
                     /translation="MKSTCHVCEKSFEARFRYQVREENGQYVYICSTACQQRLLLGAD
                     GVNACDTCGAMFEIEFPYQMSVSDGRRQYFCTPECRERGHQRLSEIGIVRPTPKRIAV
                     FNHKGGTGKTTTSINLAAGLAESGRRVLLIDADGQGNVGASLGIAGQRTLYHVLVNGA
                     KAADVAIPVRDGLDVLTSNESLAAAELFLAERPNRDRIMRERLGDACRDYDVVVVDCA
                     PALSLMNQNAMVYADSVVVPVACDYLSLVGVKQVLRTIRNVRELLQHDVQLLGVLPTF
                     FDVRNRISREAILTLRQHFEGRCYDPIRINTKLREAPSAKQTIFEYAPSSHGAEDYMR
                     LVERVTAVAATGRRAQADLLVAS"
     gene            complement(5837..7744)
                     /locus_tag="VFG22_03890"
     CDS             complement(5837..7744)
                     /locus_tag="VFG22_03890"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HET6415410.1"
                     /translation="MAYTAKDIDVLEGLEPVRKRPAMYIGGTDAHGYHHLLWEILDNS
                     VDEAINGHASRIEVALDKDHQGATVTDNGRGIPVDTHPKFKKSALELILTTLHAGGKF
                     EGKSYQVAGGLHGVGASVVNALSSDLTATVWRGGKTYSQTFKRGKAASKLKTGAKTSK
                     RGTCVHFRPDTEIFGKRAAFDPKRVLERLEAKTYLHRGLKIIFIDGKTGERQELEHPG
                     GIVDFLDKLVAERGQPSVHSEPFTAESEGKPRLEVALRWTEATDEVIRSYANGIPTGS
                     GGTHENGFKQGLAKAIRNHMDSHKLTPKGVKITADDIREGLVALVSVYVEEPQFQGQT
                     KDRLNNPEVTAQIETSVRTAFEQWLLNNKTSAESIIARVIISSRARAASRAASQQVSR
                     KTAVSHRLNLPGKLADCSSTNPDDSELFLVEGDSAGGNAKQGRDRKTQAILPLRGKVL
                     NAERASQAQILGNRELQDIVSALGCGIGKDFDINKLRYGRVFLLMDADSDGHHIATLL
                     LTFFFRMLPGLVRAGHVFLAQPPLYRLDIGKQVHWALDDQERDRIISEAPANAKVEIS
                     RFKGLGEMTADELRETTLDKTRRRALRVVVEGELEADRVFSELMGKDPAPRYQFIMEQ
                     APEADAVELDV"
     gene            complement(7744..>9044)
                     /locus_tag="VFG22_03895"
     CDS             complement(7744..>9044)
                     /locus_tag="VFG22_03895"
                     /inference="COORDINATES: protein motif:HMM:NF012731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HET6415411.1"
                     /translation="NPQVAAPQRIGLKAILRHFLDFRMEVVTKRLEFELDQLLKRIHI
                     LEGFVLVFDALDEAIQIIRRSEGKQDAAAKLMKRFGLDELQVDAILELKLYRLAKLQI
                     LEIRSELDQKLKEAQRVQKLLKSPAARWKLIRAELQELASLYADPRRTRISAKLDEPE
                     FDAEAFIIDEDAMVIVTEQGWVKRQQNLKDVASTRVRKGDRVLAVLAGSTKSTVAFFS
                     SHGVCYVSRIVDIAATTGHGTPLQTMFKMADGERIVGAMGFDPRFLDVPPLDEESGEP
                     EPPYAVAVTRGGLALRFSLRGHREPSTRSGRKFTRPKKGDEVIYVAPVFEEDCIACVT
                     KQRRALVCEAEEVSLLAGAGRGVMLIKLQKDDEVLAARVLSYEDDVLIAKRDGGSEYR
                     ITLRKYEVVSRGGKGFQLFTRGEVEGEVYQEPEIPIFPEE"
BASE COUNT         1591 a         2911 c         2799 g         1743 t
ORIGIN      
        1 tccggtccgc acctttctcc gcgcgatgga agcgttcgag cgatacgtcg accccgaccg
       61 ctgcgtgaag cttctcaagg aaacgctcgc catgcgcccg gacctcgtcc gcgcgcaagc
      121 ggacctcgtc ctcgtgagcg agagcatcga tgcacggtac gcggagctgc aggcgctcaa
      181 gaagatcagc cccacccaca tcgtcgtccg cctgtccggc cggatgatcg aagaagagta
      241 cgagaccgcc aaagagctgc agggcgcgtt caagccataa gagtggatgg agtgcccaca
      301 ctggcactgg cactggcact ggcactggca ctggcactgg cgctggcgct gacgtgcgga
      361 ggaggacggc tcgagatggg cagcgcagcg attgggtcga gcctagtcgt aggtcgtctt
      421 aaggaactgg gggcgagtgg gtcccaatcg cgagcacgcc cgtcgcgagc cgtcctccgg
      481 cgactctttc gcgcaacacg cttcctggca ccggcgctga atacccctac ggggggatcg
      541 ggcgaagctc gatcgtaatc gtgctctcgc ccttgggtag gtcgagctcc atgtcgtggg
      601 ggaagtggtc ttcggcctct acggtcatga ggtgaacgcc cggggccaga gccttgggct
      661 cctgcgacaa gacggtgcca cggccgaagt agtggccgtc gatatacacc gaggccgtcg
      721 cagggctggt gatgaccctg agtcgcgccg gctccggggc gatcgtgttg ttcttcgcgc
      781 agcctagcgc cagcaaggcg acgagcgcga gggcggcccg cctcactccc attcgatcgt
      841 cgagggaggt ttggacgaaa tgtcgtagca gacgcggttg catccccgga cctcgttgat
      901 gatgcgtgtc gagattcgag caagcacctc gtgcgggata cgggaccagt cggcggtcat
      961 gccgtcccgc gagtcgaccg cccggaccgc gatcacgtag tcataggttc gctcgtcgcc
     1021 catcacgccc actgtgcgca cgggaagaat gacgcagaag gcttgccaga tcttgtcgta
     1081 gagatcggcc gcccgaagct cctcgtcgaa gatggcgtcg gcttctcgaa gaatgtcgag
     1141 cttctcccgc tcgatcgcgc ccggacagcg aaccgcgagg ccggggccgg ggaacgggtg
     1201 ccgccagagg acatcgcgag gcatgcccat cgcctcgccc acttcgcgaa cctcgtcttt
     1261 gaagagcagc ctcagcggct cgatcagctc gaggtgcaag gtgtcgggca ggccgccgac
     1321 gttgtgatga ctcttgatga ccgcgctcgg ccctttgaaa gacaggcttt cgatcacgtc
     1381 cgggtacaag gtcccctgga cgagatagcc gacgtcgtcg agcttttccg cctcacgctg
     1441 gaagacgtcg atgaaaacgc gaccgatgat cttccgcttt ttttctgggt cggacactcc
     1501 cgcgagctga tcgaggaact cgtcggcagc gtcgacgtga atgagcttga ggccgaagtg
     1561 atcgcgatag gtgtggacca cgctctcggc ttcgtctttt cgaagcaagc cattgtcgac
     1621 gaagatgcag gtcaggcggt ctccgatggc cttggagacc agcatcgccg ctacggagga
     1681 atcgacgcca ccggacagac cgcaaacggc ggcccgcttg cccacttgtc tgcgaatcga
     1741 gtcgacgctc tgctcgacga acgagcctgg cgtccagttg gccgcgcagc cggcgacctc
     1801 gaagagaaag gcgcgcagca tctcgacgcc acggggcgtg tgcgcgacct cgggatgaaa
     1861 ctggaccccg aagatgttgc gctcgagatt cgctactgcg gcaaacgggc tgttgctggt
     1921 ccgcgcgatg gtctcgaaac cgggcggcag gctctccacc cggtcgccgt gactcatcca
     1981 gacgtcgacg tgactgcccg cctgaaatcc atgaagaagc ccttcgggct tgagcacttc
     2041 gagtcgcgcg ggcccgtact cgcgctcgtc cgcgcgctgc actttcccgc cgagcaagtg
     2101 actggtgagc tgcatcccgt aacagatgcc caagacgggc acgccgagat cgaacacgcc
     2161 ggggtcgacg gtcggcgatc cgtcggcgaa cacactcgcc ggtccgccac tgagaatgat
     2221 cgctcggggc gcgatgtcct tgacctcggc cagggaatag gtgcagggct ggatctcgca
     2281 gtaaacgtgc tgctcacgaa tccgacgagc gatgagctgc gtgtactgcg acccaaaatc
     2341 gagaatcaaa accccatcga cgatcatgcg gcgggtctaa catgcccccc cgaaccggtc
     2401 gagcgacgga acccgcagtg gccgcagcct agaacgctgg cgctgacatc ggcgctggcg
     2461 atgtgaagat cggagagcgg ctcgcgacgg gcgtgctcgc gattgggacc cactcgcccc
     2521 caattccata agacgaccca cggctaggct cgacccaatc cctgcgctgc ccatcccgag
     2581 ccgccctcct gtgtacacgt cgaagcacga gcgctgacac cggcgctggc actgggagtg
     2641 tatggagggg cgttgcggaa cgcgatgcgg gggttcggcg gcgtgtgagt gccaatcggt
     2701 cagggagcag ccacctccga ttggcgcgag ctccgagggg cgccttgcgt ggcccctgct
     2761 cgcagtccct ccggccacct ttgcaaggcg ccccgtccgt cgaacccccg tgtcgcgttc
     2821 cgcaacgccc cggttagctc acgcgataat tcggcgcttc cttagtgacc atcacgtcgt
     2881 gcacgtggct ctcgtggagc cctgcggcgc tgatgcgcac gaactcggcg ttcttttgta
     2941 gctcggcaat cgacgggttg cctgtatagc ccatgccggc gcgcagcccg ccgaccagtt
     3001 ggtacacgac cgtcgatagc gcaccgcggt acggcacgcg gccctcgatc ccctccggga
     3061 ccagcttacc tacatctgaa atgtcggact gtgcgtagcg atcggaggaa ccttcacgca
     3121 ttgcgccgag tgagcccatg ccgcgatacg acttgtaggt tcgtccctgg taaaggacga
     3181 gctcgccagg cgcctcgtcg gtgcccgcga agagagagcc gatcatcacg ctctcggcgc
     3241 cggcggcgat cgccttcacg gcgtcgccgg agtacttcac gccgccgtcc gcgatgatgg
     3301 gaacgccatg ctcgcgccct acccgcgcgc agtccatcac cgccgtcacc tgcggaaccc
     3361 cgatgccagc cacgacacgc gtcgtgcaga tcgagcctgg ccccataccg accttcacgg
     3421 cgtctgcgcc ggcgtcgatc agcgctttgg tgccagcggc ggttgcgacg ttgcccgcaa
     3481 cgacttgcac ctccgggtat gtcgatttcg tctcgcgcac tgcgtcgatg acgcccttgg
     3541 tgtgcccgtg cgcggtgtcg atgatcagca cgtctacccc tgcgcgaacc agagcctcgg
     3601 tgcggttctc tcgatccgga cccacgccga gggccgcgcc tacgcagagc cggccgagcg
     3661 agtccttgat cgaatccggg tatcgatcgg cctgcaacaa gtccttgatg gtgatgaggc
     3721 cgacgagatc gccatcgtct ccgacgacga gaagcttctc gatgcgatgc ttgtgcagga
     3781 gctcccgcgc cttgtcctgc tcgacaccgg gaggcacggt gacgagctct ttggtcatca
     3841 gctgagcgac cttctgattg aggttctgct cgaagcggat gtcacggctc gtgagaatgc
     3901 cgaccggctt ggttccttct accaccggca gccccgatat ctcgtggcgc cgcatcaact
     3961 cgagtgcgtc gtggagcgac tcccccggcc ccacggtgac cgggtcgacg atcatcccgg
     4021 tttccgctcg cttcaccttc gtgacctcga gcgcctgaag ctccgggctc atgttcttgt
     4081 gcaagatgcc gatcccgcct tcgcgggcca tggtcaccgc ggtgcgccat tcggtcaccg
     4141 tgtccattgc gctcgaaacg aacggaattc gcagctcgag accccgcgta aaacgggtcg
     4201 acacgtccgc gttcgcgggc agaaaatccg cgtaccccgg aaggagcaac acgtcatcaa
     4261 aggtgagtgc ttcccgtagg atgctgtcag tcaccggaac ctcccaagga ccctaaaaat
     4321 tggcggcgag tatagcgccc cggtgacggc acgctagtag gcgcgcggga actcgtcgat
     4381 atcgacctgg tcgagggcaa accggatgag cgcgctacgg ctgatccgcc gatggccctg
     4441 ctttttgagc ttcgccacca ttttgtccag ccgggcgagg tcttcggtgt agagcgagat
     4501 gcaaatgacg tcgtagtggt ccggcttctt cgccttgggc tttatggtct ttgcgcgtcc
     4561 gcgtcggcca cctttgtcgc cgtagaacgt gtctcgcaag accgcattca cctcgtcttc
     4621 ttcaaaaacg ctctcaaccc ctgttccttc acgtcgcttc atataccccc ctaagatgca
     4681 accaagagat cagcctgcgc tcgccggccg gtcgcggcaa cggccgtcac gcgctccacg
     4741 aggcgcatgt agtcctctgc accatgactg ctgggcgcgt attcgaaaat ggtctgcttc
     4801 gcgctcggag cttcgcggag cttggtgttg atgcgaatcg ggtcgtagca gcgtccttcg
     4861 aagtgctgcc gcagcgtgag aatcgcctct cgcgagattc gattgcgtac gtcgaagaac
     4921 gtcggaagga ctcctagcaa ttgtacgtcg tgttgaagca gctcgcgcac gttgcggatc
     4981 gtccgcagca cctgtttcac tcctactagg gagagatagt cgcaggccac aggaacgacg
     5041 acgctgtccg cgtagaccat cgcgttctga ttcatcaacg agagagcggg tgcacaatcg
     5101 acgaccacga cgtcgtaatc gcgacaagca tcaccgagac gctcgcgcat gatgcggtca
     5161 cggttcggac gttccgccaa aaacagctcc gctgcggcaa gcgactcgtt ggacgtcaag
     5221 acgtcgagtc cgtctcgaac tgggatcgca acatccgcgg ccttcgcacc gttcacgagc
     5281 acgtgataga gcgtccgctg cccagcgatt ccgagcgacg cgcccacatt gccttgcccg
     5341 tcggcgtcga tgagcagcac gcgacggccc gactccgcca atccagccgc caggttgatg
     5401 gacgtcgtcg ttttcccggt gccgcccttg tggttgaaca ccgcgattcg ctttggcgtc
     5461 gggcgcacga ttccaatctc gctcagccgc tgatgcccgc gctcgcgaca ttcgggcgtg
     5521 cagaaatact ggcgtcgacc atcgctcacg ctcatttgat acgggaactc gatctcgaac
     5581 atggctccgc aggtatcgca ggcgttgact ccgtccgcgc cgagcagcag tcgctgttgg
     5641 cacgccgtcg agcagatgta gacgtactgc ccgttctcct cgcgaacctg atagcgaaat
     5701 cgtgcttcga agctcttttc gcacacatga caagtcgact tcatcaccac tccccagaag
     5761 ttatcgaagt acttcctgaa gtggtttgac cagaacgcag agcgctggtc aaaaaaatcg
     5821 aacgacgggc gttcggctag acgtcgagct cgacagcgtc cgcctctggc gcttgctcca
     5881 tgatgaactg gtagcggggc gcggggtcct tccccatgag ctcgctaaag accctgtcgg
     5941 cctcgagctc tccttcgacc acgacgcgta gcgcccgccg tcgcgtcttg tcgagcgtgg
     6001 tctctcggag ctcgtcggcc gtcatttcac caagaccctt gaagcggctg atctcgacct
     6061 tcgcattcgc gggagcctcg gaaatgatcc ggtctcgctc ctggtcgtcg agcgcccaat
     6121 gcacctgctt tccaatgtcg agacggtaga gcggaggctg tgccaaaaag acgtgccctg
     6181 ccctaacgag gcccgggagc atgcgaaaga agaacgtgag caagagcgtc gcgatgtggt
     6241 gtccatcgct gtctgcgtcc atcagcaaga acacgcgacc gtagcggagc ttgttgatgt
     6301 cgaagtcttt tccgataccg cagcccaggg cgctcactat gtcttgtagc tcgcggttgc
     6361 cgagaatctg cgcctgcgat gcgcgttcgg cgttcaagac cttgccgcgc agcggcagaa
     6421 tcgcctgcgt cttgcggtcg cgcccttgct ttgcgtttcc gccggcgctg tcgccctcga
     6481 ccaagaagag ctcgctgtcg tcggggttgg tgctcgagca atcggcgagc ttgcccggca
     6541 agttcaaacg atgactgacg gcggtcttgc gagaaacctg ctggctcgcg gcacggctcg
     6601 ccgcgcgggc tcgggacgaa attattacgc gcgcgatgat cgattcggcg gaagtcttgt
     6661 tgttcaggag ccactgctcg aacgcggtgc gcaccgaagt ctcgatctgc gcggtcacct
     6721 cggggttgtt caggcgatcc ttggtctgac cctgaaactg cggctcctcg acgtacacgc
     6781 tcaccagcgc cacgagccct tctcgtatgt cgtcggccgt gatcttcaca cccttgggcg
     6841 tgagcttgtg cgagtccatg tggttgcgaa tggccttcgc cagcccctgc ttgaacccgt
     6901 tctcgtgcgt cccccccgag ccggtcggaa tgccattcgc gtacgaacga atcacctcgt
     6961 cggtcgcctc ggtccatcgc aaggccactt cgagccgcgg cttgccctcg ctctcggcgg
     7021 tgaacggctc actgtgcacc gatggctgac cacgctcggc gaccagcttg tcgaggaagt
     7081 cgacgatgcc tcccggatgc tcgagctctt gtcgctcgcc ggtcttcccg tcgatgaaga
     7141 tgatcttgag gccacggtga agataggtct tcgcctcgag gcgctccagg acgcgcttgg
     7201 ggtcgaacgc ggcccgcttc ccgaagatct cggtgtccgg cctgaaatgt acgcaggttc
     7261 cacgcttgct ggtcttcgcg cccgtcttga gcttcgaggc ggcctttccg cgcttgaacg
     7321 tctgcgaata ggtcttgccg ccacgccaaa ccgtcgcggt caagtccgag gagagcgcgt
     7381 tgacgaccga cgcgccgacg ccgtggagcc cgcccgcgac ctggtagctc tttccttcga
     7441 acttgccgcc tgcatgaagc gtggtgagga tcagctccaa cgccgacttc ttgaactttg
     7501 ggtgcgtgtc gaccggaatg ccgcgcccat tgtcggtgac ggtcgcaccc tggtggtcct
     7561 tatcgagtgc gacctcgatg cgtgaagcat gcccgttgat cgcctcgtcg acggagttgt
     7621 cgaggatctc ccagagcaga tgatggtagc catgcgcatc cgttcccccg atgtacatcg
     7681 cagggcgttt gcgaacgggc tccaagccct cgagaacgtc gatgtctttg gcggtatacg
     7741 ccactactcc tccgggaaga tgggaatttc gggttcttgg tagacttcgc cctcgacttc
     7801 gccgcgtgtg aagagctgaa agcccttacc ccctcgcgag acgacctcgt acttgcgcag
     7861 cgtgattcga tactcgctgc caccatcgcg cttggcgatc agaacgtcgt cttcatacga
     7921 gagcacgcgc gcggcgagga cctcgtcgtc tttttggagc ttgatcagca tgacgccgcg
     7981 ccccgccccg gcgagcagcg acacttcttc ggcctcgcag accagtgccc gcctctgctt
     8041 cgtcacgcaa gcgatgcagt cttcctcgaa gaccggcgcg acgtagatca cctcgtcgcc
     8101 cttctttggc cgggtgaact tgcgaccgga gcgagtcgac ggctcgcgat ggccccggag
     8161 cgagaagcgc agggccaggc cgccacgtgt gaccgcgacg gcgtaaggag gctcgggttc
     8221 gccggattcc tcgtcgagcg gcggcacatc gagaaaacgc ggatcgaagc ccattgcgcc
     8281 gacgatgcgc tcgccgtccg ccattttgaa catcgtctgc aagggcgttc cgtggccggt
     8341 ggtcgctgcg atgtcgacga ttcggctcac gtagcagacc ccatgactgc tgaagaaagc
     8401 cacggtgctc ttcgtcgagc ccgcgagcac ggcgagcact cggtcgccct ttcgcactcg
     8461 agtcgaggcc acgtccttca ggttctgttg gcgcttgacc cagccctgtt cggtgacgat
     8521 gaccatcgcg tcctcgtcga tgatgaaggc ctcggcgtcg aactcgggct cgtccagctt
     8581 ggcgctgatc cgcgtacggc gagggtccgc atagagagag gcgagctcct gaagctcggc
     8641 acggatcagc ttccagcgtg ccgccgggct cttgagaagc ttctgcacgc gctgggcttc
     8701 cttgagtttc tggtcgagct cggaccgaat ctcgagaatc tgtagctttg cgagacggta
     8761 cagcttcagc tcgaggatcg cgtcgacctg aagctcgtcg agcccgaagc gcttcatcag
     8821 cttggccgct gcgtcctgct ttccctcgga gcgccggatg atttgaatcg cttcgtcgag
     8881 cgcgtcgaaa acgagaacga agccctcgag gatgtggatt cgcttgagga gctggtcgag
     8941 ctcgaactcg agccgcttgg tgaccacttc catgcggaag tcgaggaagt gccgaaggat
     9001 ggccttgagg ccgatgcgct ggggcgccgc cacttgcgga ttgg
//
LOCUS       DASRZF010000089        32421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_407692, whole genome shotgun sequence.
ACCESSION   DASRZF010000089 DASRZF010000000
VERSION     DASRZF010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 32421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32421
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407692"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VFG22_03900"
     CDS             complement(<1..594)
                     /locus_tag="VFG22_03900"
                     /inference="COORDINATES: protein motif:HMM:NF020090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET6415412.1"
                     /translation="MTEPIRVGIFGWPVAHSKSPQMHESAAQALGIGLRYERFAVEPR
                     DLAKAVRREHAGRIDGYNLTVPHKEAILSLLDEVDATARAIGAVNTVVRKGERYIGHN
                     TDAPGFVRSLEEAGLSPLDAQVCILGAGGAARAAVLGLANAGASEISVLSRRPEQSQA
                     LCDSLAPHVSCKLIAKPLGEARDAFEAATLLVQASSAT"
     gene            complement(587..2026)
                     /gene="xseA"
                     /locus_tag="VFG22_03905"
     CDS             complement(587..2026)
                     /gene="xseA"
                     /locus_tag="VFG22_03905"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HET6415413.1"
                     /translation="MSEMPLFERLQGEGHDGDERVFRVAEVNRAVRVTLEDKWANVLI
                     EGELSDVRRAKGHVYFTLNDEEDPAQLRGVMFQSDVKRTKAPLEDGARIRFRGTLSLY
                     TARGQFQLIARSARLAGEGDLAAQFRKLREKLEAEGLLDPERKRSLPQLPRVVGVVTS
                     RSGAALHDIIRVASERCPVRILVADCRVQGEEAPASIVAALELIQQVDALDVVIVSRG
                     GGSAEDLWAFNDEGVARAIAGCRVPVVSGVGHEVDITIADLVADLRAATPSNAAEIVV
                     PAREALVRDVRGLERRLNQALDNRIGGLRLRLERLLRPLYGARRVISPVREELADLHE
                     QLKRAQRSELRSRRDALAELRQRLMRRDPRSILRRDQRDLAALTTKLRDAGRLPVRRR
                     RERLVALRFTLTGRGRPMVREARAKYAELCAHLDALSPLKVLERGYAIALHEPSGRAI
                     RSQSEVKQGDRLRVRVSDGEFAAKVEGDD"
     gene            2095..2925
                     /gene="folK"
                     /locus_tag="VFG22_03910"
     CDS             2095..2925
                     /gene="folK"
                     /locus_tag="VFG22_03910"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HET6415414.1"
                     /translation="MSLVVAVGSNLGAREASIRCAQALLDARDGVVVRRVSKIYETEP
                     LGLPQPKYLNAAFRLDTDLSASALLETLLRTERRLGRRRDPEARWGPRSVDLDLLWDA
                     RGRQESPGLKVPHPELERRTFALAPLLDVAPELEGRYGPALQALGGPPNEWTRPAIIR
                     RSRAPDGVHVEVEADSLADACALCLGQTPSSGRPWSTRHLRIDPSSESFAAALREVFR
                     TGFAVQRATISHCSEAQWMLELHGVNMGMPAAVDVRLLTTSGAQREIRAVLDLDRPTR
                     "
     gene            3003..5069
                     /locus_tag="VFG22_03915"
     CDS             3003..5069
                     /locus_tag="VFG22_03915"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02266 family protein"
                     /protein_id="HET6415415.1"
                     /translation="MADARKDRRTLLSLKIRYKSATLEDFIERYSNDISRGGVFIKAK
                     KPLAVGTLLKFEFILQDQSTLLHGVGRVVWRRDEGDAENPSGMGIKFIKMDPESRAVV
                     QRIAEDRAHPGVFEQGKDGAPIAPAGPSDPGHLREADRTKVRHVSEFLASALEEGGAG
                     EVARREAQAGAERARQVGHSKGDPRVAAARGAFAANPELDAQKRRSMSDAPARGAMSA
                     FGGSGLSSSARMSSSAAPAFDELDPEDDFLEEETTKVHDHFSSAFPPDADATVVAAEA
                     ASPFAVEKRLTPVVASPASSRSPLESEVPDLFGPGINDSFGPAPGEFIDASLLEPGVI
                     TVPPPADKVPEAPGIPAEAFKVSQATEPVWTTRPTPPKKEVSRGWPGLVVLAVLIAGG
                     AVAVWQLGFADDLIDLAGSYLGRSEELAKAPQRVTASPAAPTEPAAPSVAAAAPSDDP
                     SPASETGTAGSEKPSAEVKASPETSPGEVAEPVMVKFEVTSRPTGAFVSVNGTGAGRT
                     PLDLEYAAGTELTIFSKARGYLARRQQITVQAKQEPVSLVLAPLPYVIEVVSSPAGAT
                     ASSSGVGQVVTPASLRFKSMPASRTVVISKDGYKTTSQTVALDDFTEETRRLVATVDV
                     TLQPSAAAAPRPTAPAAAPVAPQPASPAPVEAKAAAETTAAPKAAVQPVAAEETPAPS
                     AEPDAP"
     gene            complement(5044..5751)
                     /locus_tag="VFG22_03920"
     CDS             complement(5044..5751)
                     /locus_tag="VFG22_03920"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET6415416.1"
                     /translation="MIPIRDLNKTRRTPFVTWALIGTNVLIFMGSLSLGRPEAEALVM
                     RFGVIPDVLVHGQWVEPRALGGSLGSWVTPFTSMFLHGGLLHLGSNMLFLHIFGDNVE
                     DVLGRGRFVVFYALCGLGAVAGQVLVDPNSMMPMIGASGAISGVLAGYMTLFPHARVV
                     TLIPVFILFYFVEIPAAVFIVLWFVLQLVQGYLSLGFLAEGGGGVAWFAHIGGFLAGL
                     LFVRVLYRRPKARRVRR"
     gene            5813..7762
                     /locus_tag="VFG22_03925"
     CDS             5813..7762
                     /locus_tag="VFG22_03925"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HET6415417.1"
                     /translation="MDAGHLLGPTGPLARGVPGYEHRPGQIRMARAVQDLLQHDGVLL
                     IEAGTGIGKTWAYLIPAALSGRRVLVSTGTRALQDQIMEKDLPALKDHLGIEIDAACV
                     KGLGNYLCLRRFNELSSSADAMQPRFARALPALRDWIETTRTGDRAALASIPEEDPIW
                     SRVVSGSDTRIGARCDYYEDCFVTRVRRRAESAQLIVVNHHLFFADLALRDTGFASVL
                     PDYDAVIFDEAHQIEDTATLFFGSRLSTAMLERLVRDARTALQSERGDKRQETRLLDG
                     VLQRSSNFFAALPAGVNGGRVPLPSEAIPQEELFALDNALAELGSFCRDLRLPRESVL
                     QIARRTEQLRNALGSLEAPGQVSWAQGSGRSPSVGSSPIDVGPLLRERLHERVPCTVF
                     TSATLTTGGDFRFLKRRLGIDAEVSEELVESPFRYEEQAALYAPKHLPDPRASDFPEV
                     AAKEILELVRMSGGGAFVLSTSLRMMRLLAERVGPELEYEWFVQGDAPKQTLLDRFRD
                     QGNAVLFATASFWEGVDVPGSALRLVVIDKLPFEVPSDPVVAARCARLDETGESSFMR
                     YLVPAAALSLKQGFGRLIRTTRDRGVVAILDSRIRRKGYGKVFLRSLPPARVCDTLEE
                     VREFLISSGTPLTMASLPSPRKEAP"
     gene            7759..9513
                     /locus_tag="VFG22_03930"
     CDS             7759..9513
                     /locus_tag="VFG22_03930"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET6415418.1"
                     /translation="MTLAAAQAHGSAAIPEARHRIEPSSKSTIDEALAILQDHKQEWV
                     ALDIDERLELLELARRGVVAVAERWAYASVEGKGMTAGTSEEGEEWMAGPATVLRNIT
                     FLMTSLRDIRDYGLPQLPKPAYARPDGQVVAPVIPANGWDGLLFQGFSAEIWMQPDVT
                     LANLAENQAEFYRKSSTKGKVALVLGAGNVSSIGPMDALYKLFVEGQVVLLKMNPVNE
                     YLGPLIDEAFEAFRERGFFRVVYGGAAEGDYISQHEAVEEIHVTGSDKTHDAIVFGVG
                     EEGRRRKAERDLRNSKRLTCELGNVSPIIVVPGPWSQKDLDFQGVNIASSVVNNSGFN
                     CTATRVIVQHDGWGKRDALLASLRKAFERAPDSSPYYPGAEERQKHFMAHHPDAEQFG
                     KRGDGHVPWTLIHHLDPDDSNEICFNVESWCGQTSEVALPAGSVVEYIEKAVEFCNER
                     VWGTLACSIIVHPKSLKDPTVAAAVERAIADLRYGAVAVNHWSGLNYAMVTPTWGAFP
                     GHTVDDIRSGQGVVHNTYMFDKPQKSVLRGPFRVWPKPAWFIDNKNAEAIGRKMTYFN
                     ADPSLMRLPGLLLASLRG"
     gene            complement(9510..9947)
                     /locus_tag="VFG22_03935"
     CDS             complement(9510..9947)
                     /locus_tag="VFG22_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET6415419.1"
                     /translation="MTVKRVSPQEADALVKNEDYVYVDVRSIPEFDAGHPTGAYNVPL
                     MHATPSGMRPNADFMSVIAAVFPKHSKLVLGCRSGNRSLRAAEALIAAGFETVVDQRA
                     GTDGARDAFGQLQEAGWHAAGLDMADEAHPERSYEALLARKGS"
     gene            complement(9991..10920)
                     /locus_tag="VFG22_03940"
     CDS             complement(9991..10920)
                     /locus_tag="VFG22_03940"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6415420.1"
                     /translation="MSDDLVLEVKGLRKVFRIGFFRKRVEAVRGTSFCVKRGEIFGLL
                     GPNGAGKTTTIKSILRLIFPTEGEIRIFGRPAGDREASMRVGYMPENPYIYQYLKPLE
                     FLDLCGRLVGMSKRERLERSEAMIDKVGLRHAIDRPIGKFSKGMMQRIGLAQALLHDP
                     ELLVLDEPMSGLDPIGRKEVRDLLLEQRDRGKTLLFTSHILSDVELLCDRVVIMQQGE
                     ITSEGQVHDLLETAGRRVEIRLSGATKTLKDSLASRGTVFDHGAGRMTLRVEGQKSVD
                     EIIRISNAAGARLDALIPERETLENLFLKDAGR"
     gene            complement(10910..11980)
                     /locus_tag="VFG22_03945"
     CDS             complement(10910..11980)
                     /locus_tag="VFG22_03945"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HET6415421.1"
                     /translation="MIRLAKPWIGDDEQRAVAAVLESGQLVQGALVERFEQAVAALVG
                     RRHGVAVSSGTAALQLALRALQIGPGDEVLCPALSWPSPAHAIRTAGAKVGFVDVDPD
                     EWNSSGAAFRAARNGKTSAAIMIDQFGNPARADEIREGLGALPIIEDAACALGSRFPD
                     GPCGSLGRISCLSFHPRKIVTTGEGGLCLTDDDDLAEQLRMLRNHGQLRGEFVVAAGN
                     FRMTEIGAALGLAQLQRLPEILARRRELAKLYQDALGDELEIQKTPAGAESNYQTFGV
                     VLPQGQDRKAVLEKLRDRGVEAGLLSFAIHKLGSFAGSGASLPIAEHLAARGLALPLY
                     PQMRNNEVGEVVKALRGVLDER"
     gene            12058..12369
                     /locus_tag="VFG22_03950"
     CDS             12058..12369
                     /locus_tag="VFG22_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415422.1"
                     /translation="MDLVKQLDALERALTRLGIRLLEEELPDEARVDGGLCRVSGELV
                     LYVSPTAPPWRRAKVLLDALRRLPHRDLWLPPEIRRLLQADDFCDKPLRLISLKREDE
                     H"
     gene            12366..13613
                     /locus_tag="VFG22_03955"
     CDS             12366..13613
                     /locus_tag="VFG22_03955"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET6415423.1"
                     /translation="MSGLGLVLSGGGARGAYEVGVLEYIFSEFLDANGNPPKVDLISG
                     TSVGAVNGAFVASSIGDVPSAIKHLVSIWTELELPHVLGFGLRQAAGLYRVLLGGSGQ
                     ARGVFDPAPLSALVGRTVRWRQLRQNIRSGALRALTVSTTHVGTGQPVIFVDAAPGVG
                     IPKSLGLHALVRRARIGQHHVLASAAIPLIFPPVEIGDEIHCDGGLRLNTPMGPSVHL
                     GMNRLLVIGLSTPHPEGGRAVKDGKYPGATYMLGKVLNAFLLDHVNTDLLEVQRINDF
                     LKDGIRAYGPEFLDRINDAASARGALAERRLLSSLVLRPTIDIGLVASEYLASNRVRF
                     GKSLGRAFISMLDVGAGADADLASYLLFDGGFAQTLIEMGRRDAHAQRAQIEAFLFED
                     PDAILSTEKTVETVAHSESKRPE"
     gene            13724..14689
                     /locus_tag="VFG22_03960"
     CDS             13724..14689
                     /locus_tag="VFG22_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET6415424.1"
                     /translation="MAQPSVAHISENATQLSQRLVNNIGLVVRGKEEVIKLAVVSLLA
                     KGHLLLEDVPGVGKTTLARALAASVGVSFRRLQCTSDLMPTDVLGGNVFNQSKSTFEF
                     RPGPIFTQVLLADEVNRTTPKTQSALLEAMDERQVSIDGETHDLEEPFFVVATQNPQE
                     FYGTYPLPESQLDRFLVRLSVGYPPPDVEREVLNRRGADPVGQLEPVMSASELRHVQQ
                     AVDSVRVDDAILDYLHRIVLATRNTPLLSVGVSTRGALGFERAARARALVDGRSYVLP
                     DDVKALAGPVLSHRVKVSGGMGGAAVRDDAERVIRELLTVVAVPL"
     gene            14702..15697
                     /locus_tag="VFG22_03965"
     CDS             14702..15697
                     /locus_tag="VFG22_03965"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET6415425.1"
                     /translation="MRIFRRRRSRRKFRLTREGRAFLFVTVGVGLAAVNTANNLLYLV
                     LGLLLSLLLVSGVLSDLALWKLQIKRNLPVRLFVGNRSIVDIALLNEKRWLSSVSVDV
                     ADEIDGVETEAARFLRVAPGTTELAPYGFEAKRRGVFELGTMRVLTRYPFGLIEKGYT
                     MYLPDEIIVYPRLLDRVATPPVRPMSGDAAPMHQTGRGSEFAGSVRFYREGDEARDIH
                     WKRTASRGELVVREHEQDSSALVTLSVDNLLASGVDEGEWRERFEQSISEVATAAAAY
                     LAQGVSVQVQSSDSVSPMVIGGTPADPIWRFLALIKPVRAHAPRRDRKQKKRRAA"
     gene            15694..17856
                     /locus_tag="VFG22_03970"
     CDS             15694..17856
                     /locus_tag="VFG22_03970"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HET6415426.1"
                     /translation="MRFAFAHKLVAYLFAGLGLIALMLGTAFSPIESAIVILGFAASW
                     FAERPLLDRSGYSRFWNVAAVVLLLVQILRALGGEPLLSVGVQYAAFLQISRLSHRKT
                     AADYQQIAILGFLHLIAGTVFSSGLDYAVVFFGFVVVMPWMLALTHVRKELETQYGPA
                     SPMLLSELESRQLATPAFFAGTTLLAVPLFVLTAAMFFAFPRVGFGFLSNLGSRTQSV
                     AGFGDDVELGGFGTIRDDPTVVMRVKMKNRPDPAPQALGLRLRGTSFDRYSQGRWSRT
                     QVAGEPLKRLQDEYIVFRPPTPEDQAYDIILDPMEEAVLFLPEGTVALQVPPLVRSGR
                     NQYRRIDYAPGLDLRYRGRQDGPLTYSAAVDTRQRGFPERIPKSLRKRYLEVPPNYER
                     VAELAREVVGEETNPFARAQRIERYLRGGGDFRYTLEQPEVKDKDPLHVFLFEARAGH
                     CEYFSTAMAIMMRSLGLPARNVTGFLGADYNPYGDYYAVRNGHAHSWVEVLIDGRWRT
                     YDPTPASGQVFAAPSGLAVKIRQMMDAMRVRWAEYVVEYNIRDQARALQGLAEWYRSL
                     RGNRGAMQSSSDDALDEDQQFGSIPFRPDWRWFVGVMSAFGVAVLFVRWRRKRRRQSR
                     AGRQLDPDRDRAVRLYLSLESSLRTAGQARPADVTPIEHAEELDRSGFAAAEEVREVT
                     AAYLAARFGEDGLPVHDYHRLRKVSRSIRAKAKRPPIA"
     gene            complement(17846..19321)
                     /locus_tag="VFG22_03975"
     CDS             complement(17846..19321)
                     /locus_tag="VFG22_03975"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET6415427.1"
                     /translation="MTDQRYHLIEKLEAGGMAEVFLGEATSVQGFKKRVAIKRVLPHL
                     ASHTNFIGMFLDEARLGARLSHANIVSVFDIGKSDNSFFIVMEFVDGTNLKKIMETLR
                     AKRQPFPLKDVIYIAMEACRGLSYAHELVDDDGNPVDLVHRDVSPPNILISKRGEVKV
                     TDFGLAKARTQLERTDPGVVKGKFSYLSPEVATGQEVDERADIFALGVCIWEMLAGRR
                     LFLGDTDYETVQAVSGAQVPSLVGAHPEVDAQFEAIMMKALARKPADRYRSARELGDA
                     LASYLFHHQMKVTPYDIASLVKAALERQKSVPPQPMMIDGMIQEELGRFTSLESDANV
                     PIDPSTSFALRLDDDGARPLEASDFVDPSSWFSGDVEVETAIEQVRDSAPAGATMGWF
                     ETKDESASIPRPARIPMGPSLNPPRRSLSSEGSQPVMTFRPPGTTPVSIVADPESQPA
                     VPKPIEKPEPMKPGGSKVAVLFLGILVLAAIGVATGWFLTQ"
     gene            19435..20106
                     /gene="rpe"
                     /locus_tag="VFG22_03980"
     CDS             19435..20106
                     /gene="rpe"
                     /locus_tag="VFG22_03980"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HET6415428.1"
                     /translation="MPRSAKPLRIAPSVLSADFGRLAAEVQAVDQAGADWIHVDVMDG
                     RFVPNITIGPLIVEAIREATEKTVDVHLMIVEPEKYVQSFASAGADIITVHAEACTHL
                     HRTVQQIRALGKRAGVSLNPHTPPDCLRYIMPDVDLVLVMSVNPGFGGQSFIPSANEK
                     LARIRTMIDESGYDIDLEVDGGVKPGVAREVVGAGADVIVAGSAVFNRDDYAAAIQAI
                     RKDAE"
     gene            20103..21503
                     /locus_tag="VFG22_03985"
     CDS             20103..21503
                     /locus_tag="VFG22_03985"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET6415429.1"
                     /translation="MNDDPALTTVAVDRKPTKRKLRKSRLVVEDGPQKGTRLDIASER
                     MTIGRSGICDLQLTDEGASGTHCEIIAKEDGFLLRDLGSTNGTWVAGVRVQEAWLEPG
                     MPLRLGNTVIRFEHGPGSIEIDLSRREQFYDLVGHGVRMREIFAVLEKVAASDLTVLV
                     RGETGTGKELVARAIHRASKRSQRRLVVQDCSAIPANLIESILFGHERGSFTGAADRK
                     RGCFEVADGGTLFLDEIGELDIGLQPKLLRVLETREVQRVGGTRMIPVDVRVVAATNR
                     DLRQMVNDGTFREDLYYRLSVVQVDLPPLRERPEDIPVLAQQFLDESSKRGPQEERKH
                     FTIMRDAMTKLQAYPWPGNVRELKNTIERAVSLADRPELGMHDLLPASQKTPPVVFPG
                     GTAEQFVDNGIPFKDAKQRVVDAFEAQYLKAVLDKHGDNISQSARAAGLTRYHLRELA
                     KRYGLRGGTSGSDEED"
     gene            21537..22769
                     /locus_tag="VFG22_03990"
     CDS             21537..22769
                     /locus_tag="VFG22_03990"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET6415430.1"
                     /translation="MTGVRFAIVAMVALAWARPSVAEITEPEAAVPAPSWAKSIEVVN
                     DGAPVMLGPSSESRRRGTVGAGTRLGFTRRVFGEGCSTGVWYQTQDELFICESNVTLS
                     PQAPGPKVDLLTGSTSRLPQRYAFIRFGGTRAYAHPSDYFRNDYLEALGKGFGIVVTG
                     EQVYEGVEFVRTRRHLWIERGSVHFVDGSAFMGARIGREEALDLAWVALRPASVYEKP
                     RGRVIKRLPRHSLVHVESSKGAWSKLVGEGWMKSAELVSPKRRDPPEGVGPTERWIDI
                     DVSQQVLVAYEGAEPRFATLVSTGRNTKQSETPLGVFQIWVKLEYSDMNDIERTDVDK
                     NYSIQDVPWVQFFKGSYGFHAAFWHDDFGRRRSHGCINLSPKDARYLFQFTGPVLPPG
                     WNAILSMPEDRPTTVRVR"
     gene            complement(22809..23237)
                     /locus_tag="VFG22_03995"
     CDS             complement(22809..23237)
                     /locus_tag="VFG22_03995"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET6415431.1"
                     /translation="MPSYENSILIEAPIGVVFAYVNEPATFPDWVNGMIEVRNVVGTG
                     EGLQYEWTFKMVGLLFRGQSVAVNYVQDECAAYQSIGMIESIWTNITEPSDRGTKLTI
                     KLEYSIPTPVLGKLAEQLTLRRNKRGLDASLLNAKEALEG"
     gene            23456..23587
                     /locus_tag="VFG22_04000"
     CDS             23456..23587
                     /locus_tag="VFG22_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415432.1"
                     /translation="MFAEYMLVTAVIVLAVGLALYGLGVPLVQAFFLTKLLILLPFP"
     gene            23603..23788
                     /locus_tag="VFG22_04005"
     CDS             23603..23788
                     /locus_tag="VFG22_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415433.1"
                     /translation="MNDEAQIKRSLRNDQHGLTTVEYIIVLGLIAVVGIAAWQKFGET
                     LIGEVEAADGYISDVVQ"
     gene            23800..24345
                     /locus_tag="VFG22_04010"
     CDS             23800..24345
                     /locus_tag="VFG22_04010"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HET6415434.1"
                     /translation="MNAVATIASGFALGVAIIGAIRDWRSGEIPNWLTLPPLLIAPLA
                     YGLAIGPEQAARSFAAACFNALVPYLLFRRGGMGGGDVKIFGALGAVTGFDLFLGLEI
                     QLAALFIAMLCALGALAWRGQLLSTLASALAQISPLLLRARHRPQLSDNLSAPIRMGA
                     PILIATGIYVAPRLLFAWGQS"
     gene            24342..25133
                     /locus_tag="VFG22_04015"
     CDS             24342..25133
                     /locus_tag="VFG22_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HET6415435.1"
                     /translation="MTRRLAATPGIREDKRGVAFLEFLIAFVPLWTFFLAVVQLAFIA
                     YADLLVKHSADAAARSAIVVLPDDPNEYGGEPEMSIDRNPFTYSDISRALGGAGEMIG
                     VSSDPNRIAAAFSQQVVSNLGRSRLNTIRLAAHVPLMSLTPADVGRDPQPSIAKSIGT
                     ERKLLSALYYQPFALAVTFPGLEGGAALGPEITVRVTYAYQCTVPLARQILCKVFDEL
                     EGKSEFADAFFPLARAFVGGRFRTLQHETTLMIHDAPYVYRTEGV"
     gene            25133..26338
                     /locus_tag="VFG22_04020"
     CDS             25133..26338
                     /locus_tag="VFG22_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415436.1"
                     /translation="MFTGTKSLYQDARGSMMLMSLFMALFAIGALYYVLGVGDAILYR
                     RLMQDGADAGAHAASVMGAKGMNLHSLLNVVMAVTTGILLVIRSVEVLLEITLGVLYG
                     LLATIVLSAKASALIGVLTPVESTIERVGDAVEQFVQVAHDALDVAHHAVQRGYPLLA
                     QVRAVDAMAFQEVYDPPVAGGFVVPLFGPRLPEGGLGLPVEKAEIGALCDRVADGLQN
                     RLGNVRSKVPKWLLKFLGGAVGGALRLGKRRTCADDIVESPRRVIETRADGSVVWLGH
                     EEFQYRAYDIGRDPNDNHWRHGELGIRAAQGGAEGGHNGPYAAHVIGRIGFAQSEYYF
                     DGVEDKSEWLWKQRWRSRLRRFRISRDWIPSTILGACSGTRGRGSIHGLSRICDLARD
                     FSINAVSAH"
     gene            26346..26879
                     /locus_tag="VFG22_04025"
     CDS             26346..26879
                     /locus_tag="VFG22_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415437.1"
                     /translation="MAESILQLVRDVKGTALAEALISLPILAAVLAGVVAFNGMYIAK
                     LEAKGWARRMAWLQAESGDCPAQSCRSPECGRIEDQIRSEGLNQPLEVQNSSFSLSAF
                     LGDLGHFFLGKITNGIGIADAAMPSTVRQDLGHQRGVTTLLCNTRSRHTDTGDSVLEH
                     VCRTGLRTTEYASEVCK"
     gene            26863..27849
                     /locus_tag="VFG22_04030"
     CDS             26863..27849
                     /locus_tag="VFG22_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415438.1"
                     /translation="MKSASRVHTPVRLAVRWPFYLLATLRLGVYLSAVSLLSALMFLR
                     AVRADVEQLIWDSGASLMQHPEATAGISRRLELNGATISFRTQTIAASLESVLDHYES
                     LCESRDAHLSAALSKRLNERSAARKTPSMLRAVTTTTARNADSGYVACLDLGSEPRDL
                     VALADGFVRFAATGNLREAGVLRYVFARRASSEPGSKTFFLTMSAESELNLHKIVPDG
                     DSDAAGRDLVGVPRPLRSQRLLSAWEVDQPSGLVLYRASGTTPEQIESFYRTELLRLG
                     WKIIERNRGESIQIDGLRMLSAEQRDRVVTILSRPGEQETALVTILGSEPTR"
     gene            27846..28607
                     /gene="cpaB"
                     /locus_tag="VFG22_04035"
     CDS             27846..28607
                     /gene="cpaB"
                     /locus_tag="VFG22_04035"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HET6415439.1"
                     /translation="MNRNAMFASVAVASVGVMLLGVYIRQFQRETSGGEPIALLAMRQ
                     DVAAGVPLNEQMLVVRRLPESYVEARQVLASDLPRVLGVRASIPLEANQTLLWTDLAG
                     TLQHRAALSTRIPRGMRAMAVTGAGRGDLGELIRPGDRVDVLLTKTKPGPETNVVTVP
                     LLQNVLVLAVGKEFDPMRKEAHSRGSDSVTLLLTVDQASLLAQARQNSTLSLALRNEN
                     DLAINEALPETNDSDILELEKRASRQRRLRIEKVD"
     gene            28672..29760
                     /locus_tag="VFG22_04040"
     CDS             28672..29760
                     /locus_tag="VFG22_04040"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HET6415440.1"
                     /translation="MRATAEIGSARELVLRVGEQRSLPADGIRSYSEGVPGIAEIRVT
                     RDQSRFVIVGKAEGSTSLLLIRSDGTQVQYTIRVVPADANKGFGERENIRLDLYFVMI
                     QDRYSHQIGLNWPGNVGSTLTPSEATFAISGGSQQVTQSAFQILPQTFLPSFDLAQAK
                     GWAKMYRQATLVTANGTEAVFTSGGEFNVQVQNNLTVNVQAIEFGTKVSVLPKYDSRT
                     GRIELEIVADVSDLDAERGIRGIPGRITSHVETLVNLELGQSVSLAGIRARTERRKKD
                     GIPGLALVPFIGALFGSHGRETEDNESYMFVVPSVVEPVSLSQRNRVEEALRAYEGFQ
                     GGVDAHQLKDQPRVGTERPSSKGRRTAQ"
     gene            29757..31340
                     /locus_tag="VFG22_04045"
     CDS             29757..31340
                     /locus_tag="VFG22_04045"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HET6415441.1"
                     /translation="MTAGFSVNVRMPDGSERNFDVTGTDSLTVGRDPSCHVVLSSPAV
                     SRFHLQVLDGEDGPRIVDQSANGTLLGSERVGATGAPLMPGGQLRIGPYLLRIAVLRA
                     ASIDGAASPELRRTIHRSLLDHLDLAALDEIDMQAAALRPRVIEALKRIVSTLDPSVC
                     PESEKERLVAEMTNEALGLGPLQELLDDDSVSEIMVVDPNTIYVEKHGKIRLTPLRFT
                     DDESCRAAIERIVTPLGRRIDESTPLVDARLKDGSRVNAIIPPLAIRGPCITIRKFAR
                     RPLLMDQLVDLGSLSPQMARFLRRCVRAKKNLIISGGTGSGKTTLLNVLSTEIDPSER
                     IVTIEDAAELRLDQPHVVSLESKPRNLEGVGGYEIRDLVKNALRMRPDRIIVGECRAG
                     EAIDMLQAMNTGHEGSMTTTHANSAGEALKRIETLCMMAGLDLSPRTIREQIASSIHI
                     VVQQSRFSDGTRRLTSVTEVGPLGPDGELVLHDIFAFQRTGTSASGEVVGVHRTTGYV
                     PSFLDEFIAQGLIDGGDYL"
     gene            31337..32197
                     /locus_tag="VFG22_04050"
     CDS             31337..32197
                     /locus_tag="VFG22_04050"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET6415442.1"
                     /translation="MRLESLPLLILAAACLVVGCGLITYSALSLEDTPVRRLFLRYAG
                     RLDRHSGFLLMSQSGREIARMQVIAGAPVAAFSIVARSPGLALVAVAIFAIPPFVLWK
                     RHEARVAQLERQLGTWLLMLTNALKATSSVGEAIASTVALVPKPFSEEVDLLVKEIRL
                     GAPLDRAVSALARRINSTVVSGALSTIIVARRTGGDLPKTLERASAALRESARLEGVL
                     RTKTAEGRGQVTVLASVPFVLCVIIAWLDRSWFDPMIHHPIGKGILLSCAGAWVFATG
                     WAYHIIKADL"
     gene            32194..>32421
                     /locus_tag="VFG22_04055"
     CDS             32194..>32421
                     /locus_tag="VFG22_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415443.1"
                     /translation="MTERLNILLALSVITIFGGTAGVCLQLAKRPAVPRPSLGARGVK
                     RARALRHSALFALTEPPLRALAAWIAILPIAV"
BASE COUNT         6110 a        10253 c         9864 g         6194 t
ORIGIN      
        1 cgtggcgctg ctcgcttgaa cgagcaaggt cgccgcttcg aaagcgtcgc gggcctcacc
       61 gagcggcttt gcaattagct tgcacgagac gtgtggagcc aacgagtcgc agagtgcctg
      121 gctctgctcg ggtcggcgcg agagcaccga gatctccgag gcgccagcgt tcgcgagccc
      181 aagcactgcg gcgcgtgccg ccccgccagc tccgaggatg cagacctgcg catcgagcgg
      241 gctcagtccg gcctcctcga gcgagcgaac gaatccgggc gcgtcggtgt tgtgcccgat
      301 gtagcgctcg cccttgcgga ccaccgtgtt caccgcgccg atggcgcgcg cagtggcgtc
      361 gacttcgtcg agaagtgaca ggatcgcctc tttgtgaggc accgtgaggt tgtagccgtc
      421 gatccgcccc gcgtgctctc gacgaacggc ctttgccaag tccctgggct ccacggcgaa
      481 acgctcgtat cggagcccga tgccgagagc ctgcgcagcg gactcgtgca tctgaggcga
      541 tttcgaatgg gccaccggcc agccgaaaat gccaacgcgt atcggttcag tcatcgcctt
      601 cgaccttggc agcaaactcg ccatccgaca ctcggacgcg caggcgatcc ccttgcttca
      661 cttccgattg gctgcgaatc gcacggccgc tcggctcgtg aagcgcgatc gcgtacccgc
      721 gctccagaac cttcaaggga gacagtgcgt cgagatgcgc gcagagctcc gcgtactttg
      781 cacgcgcttc acgcaccatg ggccggcctc ggccggtcag ggtgaagcgc agtgccacga
      841 gtcgttcgcg tctgcgccgg accggcaagc ggcccgcgtc tctcaatttc gtggtcaggg
      901 ccgcgagatc gcgctgatca cgtcggagga tcgatcgcgg gtcccggcgc atcaggcgct
      961 gtcggagctc ggcgagcgca tcgcggcggc tccggagctc ggagcgctgc gctcgcttca
     1021 gttgctcgtg cagatcagcg agctcttcgc gcacgggcga aatcacgcgg cgcgcgccgt
     1081 agagcggcct cagaagccgt tcgagacgaa gccggagccc accaattcgg ttgtccagag
     1141 cctggttgag ccgccgctcc aatccacgga cgtcgcgaac cagagcctct cgcgcgggca
     1201 cgacgatttc tgccgcattg gacggcgtgg cagcacgcag gtctgcgacc aggtccgcga
     1261 tggtgatgtc cacctcgtga cctacgccgc tcacgaccgg aacccggcac cccgcgatcg
     1321 cacgcgccac gccttcgtcg ttgaacgccc agaggtcttc ggcggagccg ccaccgcgcg
     1381 aaacgatgac gacgtcgagc gcgtcgacct gctgaatcag ctcgagcgcg gcgacgatgc
     1441 tcgcgggcgc ctcttctcct tgcacccgac agtcggcgac cagaattcgc actggacagc
     1501 gctccgacgc cacccgaatg atgtcatgca gagcggcgcc gctgcggctc gtgaccacac
     1561 ccaccacccg cggcaactgc ggaagcgaac gtttgcgctc cggatcgagc agcccctcag
     1621 cctcgagctt ctcgcgcagc tttcgaaatt gcgccgcgag atcgccttcg cctgcaagcc
     1681 ttgcggagcg cgcaatcagt tggaattgcc cccgagccgt atacaaagag agggtccctc
     1741 ggaaccgaat ccgtgcgccg tcttcgaggg gtgccttcgt tctcttcacg tccgactgga
     1801 acatgacgcc gcgaagttgc gcagggtcct cctcgtcgtt gagcgtgaaa tacacgtgtc
     1861 cttttgcgcg ccggacatcc gaaagctcgc cttcgatcag cacgttcgcc cacttgtcct
     1921 cgagcgtgac gcggacggcg cggttgactt cagccacgcg gaatacacgt tcatccccat
     1981 catggccttc gccctgaaga cgctcgaaga gcggcatctc cgacatgccg ggaccctagc
     2041 cgcaatcaag cgggtgaaaa aggggccagt tctcttttcg tgtccggagg tctcgtgagt
     2101 ctcgtcgtgg ccgtgggatc gaacctcggg gcgcgtgagg catcgattcg atgcgcgcag
     2161 gccctcctcg atgcgcgcga cggcgtcgtc gttcgtcgtg tttccaagat ctacgagacc
     2221 gagcccttag ggttgccgca accgaagtac ttgaatgcag cgtttcgtct cgataccgac
     2281 ctctcggcga gcgcgctcct cgaaacgttg ctccgtaccg agcgccgcct cggacgacgc
     2341 cgtgatccgg aagcgcgctg gggtccgcgc agcgtcgatc tcgatttgct ttgggacgcg
     2401 cgcggcaggc aggaatcacc cgggctcaaa gtgccacacc cggagctcga gcggcgcacg
     2461 tttgcactcg cgcccctcct cgacgtcgcc cccgagctcg aaggtcgtta tggacccgcg
     2521 ctccaggccc tcggtggccc gccgaacgaa tggacccggc ccgccatcat tcgcagatcg
     2581 agagctccag atggtgttca cgtcgaggtc gaggccgact cactggccga cgcctgcgcc
     2641 ctctgtcttg ggcagacgcc ttcctcggga cgcccctggt cgacgcgcca cctcaggatc
     2701 gatccgagct ccgaatcttt tgctgctgcg ctgcgcgaag tttttcgcac cggattcgcg
     2761 gtccagcgcg caacgatcag ccactgttcc gaagctcagt ggatgcttga attacatggc
     2821 gtaaacatgg ggatgccagc cgcagtcgat gtgcgacttc tgaccacctc cggcgcccaa
     2881 cgcgaaatcc gagccgtcct ggatctcgac cgtccgaccc gctgaagctc ttttttgatg
     2941 cgattcgttt gttgcttcac gaacggctgg ctaatctgga cttgggggtt tcacacaaca
     3001 gaatggcaga cgcacgcaaa gacaggcgca cgctgctttc gctgaagatc cgctacaaga
     3061 gcgcaacgct cgaggatttc atcgaacgct acagcaatga tatctcgcgc gggggtgtgt
     3121 tcatcaaagc gaagaaaccg ctagcggtgg gcacgcttct gaagttcgag ttcatccttc
     3181 aagatcagtc gacgctgctg cacggagtcg gtcgagtcgt ttggcgacgc gacgaaggtg
     3241 acgcagagaa tccgtcgggg atggggatca aattcatcaa gatggatccc gagtcacgcg
     3301 ccgtcgtgca acgcattgcg gaagaccgag ctcaccccgg cgtcttcgag cagggcaaag
     3361 acggcgctcc cattgcaccc gcggggcctt ccgatcctgg gcacctgcgt gaagcggatc
     3421 gaaccaaggt ccggcacgtg agcgagtttc tggcgtccgc gctcgaagaa ggcggcgcag
     3481 gagaggttgc gagacgagaa gctcaggctg gcgcggagcg cgcccgccaa gtcggccatt
     3541 ccaagggcga cccacgagtc gctgcggcac gcggcgcttt cgctgccaat ccagagctcg
     3601 acgcgcagaa gcggcgctcg atgtccgatg ctccggcgag aggcgccatg tcggctttcg
     3661 gcggatcggg cctcagctcg agcgcgcgca tgagcagctc ggcagcgccg gctttcgacg
     3721 agctcgaccc cgaagacgat ttcctcgaag aagagaccac caaagtccac gatcatttct
     3781 cttcggcctt cccgccagac gcagacgcta ccgtggtggc cgcggaagct gcatcgcctt
     3841 tcgccgtcga aaagcgactc acgcccgtgg ttgcatcgcc cgcttcttcg aggtctcccc
     3901 tcgagagtga agttcccgac ctcttcggcc cgggcataaa cgactcgttc ggccctgcac
     3961 ctggggagtt catcgacgcg agcttgctcg agccaggagt catcacggtg cctccgccgg
     4021 ccgacaaggt cccggaagcc ccgggaatcc ccgcagaggc gttcaaggtt tcgcaggcga
     4081 ccgagcccgt ttggaccacg agaccgaccc cgccgaaaaa agaggtgtcc cgcggctggc
     4141 cggggcttgt cgtcttggcc gtgttgatcg ccggtggcgc ggttgccgtg tggcagctcg
     4201 gcttcgcaga cgatttgatc gacttggcgg gaagctatct cggccggtcc gaggagctcg
     4261 ccaaagcgcc gcagcgcgtg accgcgtcac ccgcagcacc cactgagcca gctgcgccaa
     4321 gcgtggcagc cgccgctccc agcgacgatc cgagccccgc ctcggagacc ggtacggccg
     4381 ggagcgagaa gcccagcgct gaagtaaagg catcgccaga gacatctccc ggcgaggttg
     4441 ccgagccggt gatggtgaag ttcgaggtga catctcgccc gactggtgcg ttcgtgagcg
     4501 tgaacggaac cggcgcgggc cgcacgccac tcgatctcga atatgctgcg ggaacggagc
     4561 tcacgatctt ctcgaaagcg cgtggatacc tggcccgaag acagcagatc acggttcaag
     4621 caaagcaaga accggtcagt ctcgtgctcg ctccgctgcc ctatgtgatc gaggtcgtca
     4681 gcagcccggc gggcgccacg gcaagctctt cgggtgtcgg acaggtggtc acgccagcct
     4741 cgctccgatt caagtcgatg ccggcgtcac gcacggtcgt gatttccaag gacgggtaca
     4801 agacgacgag tcagacggtc gctttggacg acttcacgga ggagacccgg cgtttggtcg
     4861 cgacggtcga cgtgaccttg caaccgagcg cagcggcggc gccaagaccc accgctccgg
     4921 cggccgcacc ggtcgctccg cagccggcat ctcctgcacc agtcgaagcc aaagctgcag
     4981 cggagactac agccgcgccc aaggcggccg tacagccagt ggctgccgaa gagacgccgg
     5041 cacctagcgc cgaacccgac gcgccttagg ccgtcgatac aagactcgaa cgaagagcaa
     5101 gccggctagg aagccgccga tgtgcgcgaa ccaggccaca ccgccgccgc cttcagcaag
     5161 gaagcctagc gataggtagc cctgcaccag ctgcagcacg aaccagagca cgatgaaaac
     5221 tgccgctggg atctctacga agtagaacag aatgaaaacg gggatcagcg tgaccacgcg
     5281 cgcgtgggga aacaacgtca tataccccgc cagaacgcct gaaatcgccc ctgacgcccc
     5341 gatcatcggc atcatgctgt tcgggtcgac gaggacctgt cccgcgacgg caccaaggcc
     5401 acagagcgcg tagaacacga cgaaacgacc gcgtccgagc acatcctcga cgttgtctcc
     5461 gaagatgtgg agaaagagca tgttgctccc gaggtgaagc aggccaccgt gtagaaacat
     5521 gctcgtgaac ggcgtgaccc agctgccgag agaccccccg agcgcccgtg gctcgaccca
     5581 ctggccgtgc accagcacgt ccgggatgac accaaagcgc atcaccagcg cttcggcctc
     5641 gggccgcccc aggctcaggc tgcccatgaa tatcagtacg ttggtgccga tcaaggccca
     5701 tgtgacgaaa ggcgttcgcc gcgtcttgtt gaggtcgcgg atcgggatca cggtccgaat
     5761 ctctatagcg cgtcgctgag gcaacgaaag cgccgcgtgc tagagcgggt cgatggatgc
     5821 gggtcacctg ctcggaccta ccggaccctt ggcccgcggc gtgccgggct acgagcaccg
     5881 ccccggccag atccgaatgg cgcgcgccgt gcaagacctc ctccagcacg atggcgttct
     5941 cctcatcgag gccgggactg gcatcggcaa gacctgggct tacctgatcc ccgcggccct
     6001 cagcggccgt cgtgtcctgg tgtccacggg cacgcgggcc ttgcaggacc agatcatgga
     6061 gaaggacctg cctgctctca aggaccacct cggcatcgaa atcgatgcgg cctgcgtaaa
     6121 aggactcggc aattacctct gcctccgccg gttcaacgag ttgtccagca gcgctgatgc
     6181 gatgcagccg cgattcgcac gcgcccttcc cgcacttcgc gactggatcg aaaccacgcg
     6241 aaccggtgac cgcgcggcgc tggcctcgat ccccgaagaa gatcctatct ggtcgcgggt
     6301 cgtgagcgga tccgacaccc gaattggcgc tcgctgcgac tattatgagg actgcttcgt
     6361 gacacgtgtg cgccgccgag cggaaagcgc acaactcatc gtagtgaacc atcacctgtt
     6421 ctttgccgac ctcgcgcttc gcgacaccgg ttttgcctcg gtgttgccgg actacgacgc
     6481 cgtgatcttc gacgaagcgc atcagatcga agacaccgcc actctgtttt ttggctcacg
     6541 tctgtcgacc gcgatgctcg agcgtctggt ccgcgacgcg cgcacggcgc ttcagagcga
     6601 gcgcggtgac aagcggcaag agacccgttt gctcgacggc gtcctccagc ggtcctcgaa
     6661 cttcttcgcc gcactccctg ccggggtgaa cgggggacgc gtgccgctgc cctccgaagc
     6721 gatcccccag gaggagctgt tcgcgctcga caacgcgctt gccgagcttg ggtcgttctg
     6781 tcgcgacctt cgcctgccgc gcgagagcgt cctgcaaatt gccaggcgga ccgaacagct
     6841 ccgcaatgcg ctgggctccc tcgaagctcc gggtcaggtg agctgggcgc agggaagcgg
     6901 ccgcagtccc tccgtcggct cgagcccgat cgatgtgggc cctttgctcc gagagcgcct
     6961 gcacgaacgg gttccgtgca ccgtcttcac cagcgcgacc ttgacgacag ggggagattt
     7021 tcggttcctc aaacgccgtc tcggcatcga cgccgaggtc agcgaggagc tcgtcgaatc
     7081 tcccttccgc tacgaagagc aagcggctct ttacgctccc aagcatctgc ccgatccccg
     7141 tgcctccgat ttcccggagg tggctgccaa agagattctc gagctcgtcc ggatgtctgg
     7201 cggaggagcc ttcgtcctct cgacctcgtt gcgcatgatg cggctgctcg cagagcgagt
     7261 cggaccggag ctcgaatacg agtggttcgt tcaaggcgat gcgcctaagc aaacgctgct
     7321 cgatcggttt cgcgaccagg ggaacgccgt cttgttcgca acggccagct tttgggaggg
     7381 cgtcgacgtg ccagggtctg ctcttcggct cgtggtcatc gacaagcttc cattcgaagt
     7441 cccgagcgac ccagtcgtgg ccgcccgctg cgcgcgcctt gacgagaccg gcgagtcttc
     7501 gttcatgcgt tatctcgtgc cggcggcagc gctctccctc aaacagggct tcggacgact
     7561 gattcggacc actcgcgacc gaggcgtcgt cgccattctc gactcgcgaa ttcgccgaaa
     7621 aggctacgga aaagtctttc tcaggagcct cccacctgcg cgagtctgtg acaccctcga
     7681 agaggttcgg gagttcttga tctcttcggg gacacccctc accatggctt cgctgccctc
     7741 accgcgaaag gaggcaccgt gaccctagcc gctgcgcaag cacatggatc cgctgcgatc
     7801 cccgaagcga gacatcgaat agagccgagc tccaagtcca cgatcgacga agcgttagcc
     7861 attctgcaag accacaagca ggaatgggtt gcgctcgaca tagacgagcg cttggagttg
     7921 ctcgagctgg ctcgaagagg cgtcgtcgcc gttgccgagc gctgggcgta cgcctccgtc
     7981 gaaggcaagg gcatgaccgc gggaacctcc gaagagggag aggaatggat ggcaggtccg
     8041 gcgaccgtcc tcaggaacat cacgtttctg atgacatcgc ttcgggacat ccgcgactac
     8101 ggtttgcctc agctccccaa gcctgcctac gcacgtccgg acggccaggt cgtcgccccc
     8161 gtcattcccg ccaacggatg ggatgggctt ctcttccaag ggttcagtgc ggaaatctgg
     8221 atgcagcccg atgtgacgct cgccaatctc gccgagaatc aggccgaatt ctaccgcaag
     8281 agctcgacga aagggaaagt tgccctggta ctgggcgcgg gcaacgtctc atcgatcgga
     8341 cccatggacg ccctctacaa gctcttcgtg gagggtcagg tcgtcctcct caagatgaat
     8401 cccgtgaacg agtacctcgg tccgctgatc gacgaagcgt tcgaggcgtt tcgtgagcgc
     8461 gggttctttc gagtcgtgta cggaggcgcg gccgaaggag actacatcag tcagcatgag
     8521 gccgtagagg agatccacgt caccggttcc gacaagacac acgatgccat cgtctttggt
     8581 gtcggtgagg agggccgccg tcgcaaggcc gagcgtgacc ttcggaacag caagcgtctc
     8641 acctgcgagc tcggcaatgt cagcccgatc atcgtcgtcc cgggcccctg gtctcaaaaa
     8701 gacctcgatt tccaaggagt caacatcgct tcaagcgtgg tgaacaactc ggggttcaac
     8761 tgcacggcga cgcgagtcat cgttcaacac gacggctggg gcaaacggga cgcgcttctc
     8821 gcgtcgctac gcaaagcatt cgagcgcgcc ccggatagca gtccgtacta cccgggagca
     8881 gaagagcggc aaaagcattt catggcgcat cacccggatg ccgaacagtt cggcaagcga
     8941 ggcgacgggc atgtgccctg gaccctgatc caccacttgg accccgacga ctccaacgaa
     9001 atatgcttca acgtcgaatc ctggtgcggc cagacgagcg aggtggcgct gcctgcgggc
     9061 tcggtcgtcg aatacatcga aaaagccgtc gaattttgca acgaacgggt ctggggaaca
     9121 ctggcgtgta gcatcatcgt gcacccgaaa tcgctcaaag accccaccgt ggcagcggcc
     9181 gtcgaacgtg cgatcgccga cctccgctat ggcgcggtgg cggtgaacca ttggtcagga
     9241 ctcaactacg cgatggtgac gccgacctgg ggcgccttcc cagggcacac ggtcgatgac
     9301 atccgttcgg gacaaggcgt ggtgcacaac acgtacatgt tcgacaagcc ccagaagtcc
     9361 gtgctccgag gacccttccg agtatggccg aagcccgcgt ggttcatcga caacaagaac
     9421 gccgaagcga tcggccgcaa gatgacctac ttcaatgccg acccgtcact catgaggctt
     9481 ccggggcttc tcttggcgag tctccgcggc tagctaccct tgcgcgcaag tagcgcttcg
     9541 tagctccgct cgggatgcgc ctcgtcggcc atgtccagac cggctgcgtg ccagccggcc
     9601 tcttgaagct ggccgaacgc atcgcgtgcg ccgtcggttc cggcgcgctg atcgaccacc
     9661 gtttcgaagc ctgccgcgat cagcgcctcc gccgcgcgaa gcgagcggtt gccagacctg
     9721 caaccgagca cgagcttgct gtgctttggg aagaccgcag cgatgaccga catgaagtcc
     9781 gcattgggtc gcatgcccga cggcgtcgcg tgcatcaaag gaacgttgta agcgccggtt
     9841 ggatgccctg catcgaactc tggaatcgag cggacgtcga cgtagacgta gtcctcgttc
     9901 ttcacgagtg cgtcggcctc ttgcggtgaa accctcttga cggtcatgcg gtagatctag
     9961 ggaagtccga cgcgggagca agtaggccga ctagcgaccc gcgtccttca agaagagatt
    10021 ttcgagcgtc tcccgctcgg ggatcagggc atcgagccgc gcaccggcgg cattcgagat
    10081 tcgaatgatc tcgtccaccg atttctgccc ttccacgcga agggtcatgc gtccggcccc
    10141 atgatcgaag accgtcccgc gagaagccag cgaatccttt agcgtcttcg tggcaccgct
    10201 caggcgaatc tcgaccctgc gccccgccgt ttcgagcagg tcgtgcacct gaccctccga
    10261 ggtgatctca ccttgctgca tgatcaccac tcggtcgcaa agaagctcta cgtcgctcag
    10321 aatgtggctc gtgaagagga gagtctttcc gcgatctctc tgctcgagga ggaggtctcg
    10381 gacctccttg cgcccgatcg ggtcgagccc gctcatcggc tcgtcgagca cgagcagctc
    10441 gggatcgtga agaagcgcct gtgcgagacc gatgcgctgc atcatgccct tcgaaaactt
    10501 cccgatcggc cggtcgatcg catgccgaag gcccactttg tcgatcatcg cttcggagcg
    10561 ttcgagccgt tcgcgcttgg acatcccgac caatcgcccg cagaggtcca ggaactcgag
    10621 cggcttcaag tactggtaga tgtacgggtt ctccggcatg tagcccaccc gcatcgatgc
    10681 ctcgcgatca cctgcaggcc gaccaaagat gcggatctcg ccctccgttg ggaagatcag
    10741 gcggaggatc gatttgatcg tagtggtctt tccggcaccg ttcgggccca ggagcccgaa
    10801 gatctcccct cgcttcacgc agaagctcgt gcccctcaca gcctctacgc gttttcgaaa
    10861 gaagcctatg cgaaagacct ttcgaaggcc ctttacctcc aagacgagat catcgctcat
    10921 cgagcacccc tcgaagagcc ttcaccacct cgccgacctc gttgtttcgc atctgcggat
    10981 agagcggaag ggccaatccc cgcgcggcga ggtgctcggc gatcggtagt gaggcaccgc
    11041 ttcccgcgaa gcttcccagc ttgtggatcg caaacgacag gaggcctgct tctacgccgc
    11101 gatcccgaag cttctcgagg accgctttac ggtcctgtcc ctgcggcaga accaccccga
    11161 aagtctggta gttcgactcg gcgcccgctg gcgtcttctg tatttcgagc tcgtcgccca
    11221 aagcatcttg gtagagcttg gcgagctcgc gccgcctcgc gaggatctcc ggaagccgct
    11281 gaagctgcgc aagtcccaag gccgctccga tctcggtcat acgaaaattc ccggctgcga
    11341 ccacgaactc cccgcggagc tgcccgtgat tgcgcaacat tcgaagctgc tccgccaggt
    11401 cgtcgtcgtc ggtgaggcaa agcccaccct ctccggtcgt cacgatcttt cgcggatgaa
    11461 agctcaagca ggagatcctt ccgaggctcc cgcaaggtcc gtccggaaat cggctcccca
    11521 acgcgcaggc cgcatcttcg atgatgggaa gcgcgccgag cccttcgcgg atctcgtcgg
    11581 cccgcgcagg gtttccaaac tggtcgatca tgattgccgc gctcgttttt ccgtttcgcg
    11641 ccgctcgaaa tgccgcgccc gagctgttcc attcatccgg atcgacatcg acgaacccga
    11701 ccttggcccc ggcggtgcgg atcgcatgtg caggactagg ccaactaagc gccgggcaga
    11761 gcacttcgtc gcccggcccg atctgcaacg cccgcaaagc gagctggagc gccgcggtcc
    11821 cgctcgacac cgcaacgccg tgccggcgcc cgaccagcgc cgccacggcc tgctcgaaac
    11881 gctcgaccag agcgccctgt accaactggc cggattcgag aacggccgcc acagcgcgct
    11941 gttcatcgtc gccaatccag ggttttgcta ggcgaatcaa ggtcgggaca tcttatcagc
    12001 tcgacctgcg atagctccag attgcggggc gttccccccg agcacaccct aggtagcgtg
    12061 gacctcgtca aacagctcga cgccctggaa cgagctctga cgcgcctcgg aattcgcctt
    12121 ctcgaagagg agcttcccga cgaggcccgc gtcgacggtg gtctttgcag ggtttcaggg
    12181 gagctggtgc tctacgtttc gcccacggcc cctccctggc gcagggcaaa agttctactc
    12241 gatgcgcttc gccgattgcc tcatcgtgac ctctggctcc cgcccgagat ccgtcggcta
    12301 ctccaggcag atgatttctg cgacaagcct cttcgcctga taagcttaaa gagagaggac
    12361 gaacattgag cggacttggc ttggtacttt caggaggcgg cgcgcgaggg gcctacgagg
    12421 taggtgttct cgaatacatc ttcagcgagt tcctcgacgc gaatggcaac ccgcccaaag
    12481 tcgatctgat ctccggcact agcgtcgggg ccgtcaacgg cgccttcgtc gcatcgtcga
    12541 ttggcgacgt tcctagcgcg atcaagcacc tggtgtcgat ttggaccgag ctagagcttc
    12601 cgcacgtgct tggcttcggc ctgcgacagg cggcgggtct ctatcgcgtg cttctcggcg
    12661 gtagcggcca ggcacggggc gtgttcgatc ccgctcccct ctccgccctc gtcgggagga
    12721 ctgttcgctg gcgccagctc cgccagaaca tccgcagcgg cgcccttcgc gcgctcaccg
    12781 tgagcaccac acacgtcggc actggccaac cggtgatctt cgtggatgcc gctccgggcg
    12841 tcggaattcc gaagagctta gggcttcatg cgctcgttcg aagagcccgc atcggccagc
    12901 accacgtgct cgcctcggcg gccattccac tgatctttcc cccggtcgag atcggcgatg
    12961 agattcactg tgacggaggc cttcggctca acacaccgat ggggccttcg gttcaccttg
    13021 ggatgaaccg cctcttggtc atcggattgt ccacgccgca tccagagggt ggtcgagccg
    13081 tgaaggacgg caagtatccc ggcgccacct acatgctcgg aaaggtgctc aatgccttcc
    13141 ttctcgacca cgtgaacacc gaccttctcg aggtccaacg catcaacgac ttcctgaagg
    13201 acggtattcg cgcgtacggt ccggagttcc tcgaccggat caacgacgcc gccagcgcgc
    13261 gcggcgcact cgcagagcgt cgcttgctca gctcgttggt attgcgaccc acgatcgaca
    13321 tcggcctggt cgcatccgaa tacctcgcat ccaatcgggt tcgtttcggc aagagcctcg
    13381 gccgggcctt catcagcatg ctcgatgtgg gcgccggcgc cgacgcagac ttggcgagct
    13441 acctcttgtt cgatggcgga ttcgcacaga cgctcatcga gatgggacgc cgggacgcgc
    13501 atgctcagcg cgcgcaaatc gaagccttcc tgttcgaaga ccccgacgcg atcctttcca
    13561 cagaaaagac tgtggagacc gtggcacatt ccgagtccaa gcggccggaa tagctcacgc
    13621 tcctggcggc agaagggctg tggacaaaaa ggggaacgct gaccataagg tcagtgcgat
    13681 tgtaagattt tacccatagc ggtaggtgtg atacgctcca ttggtggccc aaccaagcgt
    13741 cgcgcacatc tccgaaaacg cgactcaact ctcccagcgc ctcgtcaata acatcgggct
    13801 ggtagttcgc ggaaaagaag aagtcatcaa gctggctgtc gtctcgcttt tggcgaaggg
    13861 gcatctcctc ctcgaagacg tgcccggcgt cggtaagacg acacttgcgc gcgctctggc
    13921 tgcatctgtc ggcgtgtcgt tcaggcggct gcaatgcacc tcggatttga tgcccaccga
    13981 tgtgctcggg ggcaacgtat tcaatcagtc gaagagcacg ttcgagtttc ggcccgggcc
    14041 gatcttcacc caggttctct tggccgatga ggtgaaccga acgacgccca agacacaaag
    14101 tgcgctgctc gaagcgatgg atgagcgtca ggtttcgatc gacggggaaa cccacgatct
    14161 cgaagagcca ttcttcgtcg tcgcaaccca gaatccgcag gagttctacg gcacgtatcc
    14221 tctccccgag tcgcagctcg accgcttcct cgtacggcta agcgttggat atcctccgcc
    14281 cgatgtagaa cgcgaagttc tcaatcggcg tggcgccgat cccgtggggc agctcgaacc
    14341 ggtcatgagc gcgagtgaat tgcggcacgt tcagcaagcg gtcgactccg tgcgcgtcga
    14401 cgacgcgatc ctcgactacc tgcatcgcat cgtgcttgcg acgcgaaaca cgcccctcct
    14461 atcggtcggg gtatccactc gcggcgccct aggcttcgag cgggctgcgc gagccagggc
    14521 tttggtcgac ggccgctcct acgtcctacc agacgacgtc aaagcactgg ccggtccggt
    14581 cttgtcgcac cgagtgaagg tcagcggagg aatgggagga gctgccgttc gcgatgacgc
    14641 ggaacgcgtg atccgcgagc tgctcaccgt cgtggctgtt cctctctagg cgatccggtc
    14701 gatgcggatc tttcgacgaa ggcggagcag acggaagttt cgactgaccc gtgaaggtcg
    14761 agcattcctc ttcgtcacgg tgggagttgg tcttgcggcg gtcaacacgg ccaacaacct
    14821 cctctatctc gttcttggtc ttcttctgag tctcttgctc gtgagcgggg tgctcagcga
    14881 cctcgcgctt tggaaactgc agatcaaacg aaatcttccc gtgcggctct tcgtgggaaa
    14941 tcgctcgatc gtggatatcg ccctgctgaa cgagaagcgc tggctctcga gcgtctccgt
    15001 cgacgtcgcc gacgaaatcg acggcgtgga aacagaagcc gcgcgattcc ttcgcgtcgc
    15061 cccgggaacg acggagctcg ccccctacgg attcgaagcg aaacgacgcg gcgtcttcga
    15121 gctgggaacc atgcgggtcc tcacgcggta tccgttcggt ctcatcgaga agggctacac
    15181 catgtatctt cccgacgaga tcatcgttta cccacgtttg ttggaccgtg ttgcgacgcc
    15241 gccggtccgg cccatgtcgg gagacgctgc gcccatgcac caaaccggcc gcggcagcga
    15301 atttgctgga agcgtgagat tctatcgcga aggcgacgag gcacgggaca ttcactggaa
    15361 gcgaaccgcc agccgaggcg agctcgtcgt acgcgagcat gagcaggatt cgagtgcgct
    15421 cgtcaccttg agcgtcgaca acctgctcgc gtcgggcgtc gacgagggcg aatggcgtga
    15481 gcgcttcgag cagtcgatct cggaggtcgc gaccgcagcc gccgcgtacc tggctcaggg
    15541 cgtatcggtg caggtgcaga gcagcgacag cgtctcgccg atggtcatcg gcggcacccc
    15601 ggcggaccca atttggcggt ttttagcgct gatcaagccg gtgcgcgctc atgcgccgcg
    15661 tcgcgatcga aagcagaaga agaggcgcgc cgcatgaggt ttgcctttgc gcacaagctc
    15721 gtagcctacc tgtttgctgg tctcggcctg atcgctctga tgctcggcac tgcgttcagc
    15781 ccaatcgaat cagcgatcgt gatcctgggg tttgctgcaa gctggtttgc ggaacggccc
    15841 ctcctcgatc gatccgggta ttcgcgattt tggaacgtcg cggcagtcgt gctcctcctc
    15901 gtgcaaatcc ttcgtgccct cggcggcgaa ccactcttgt ccgtcggcgt gcagtatgct
    15961 gccttcttgc agatttctcg attgtcgcac cggaagacgg ctgcggacta ccagcagatc
    16021 gcgatcctcg ggttcttgca cctcatcgca gggacggtgt tttcctccgg tctcgactat
    16081 gccgtcgtct ttttcggttt cgtcgtcgtg atgccctgga tgctcgcgct gacgcacgtt
    16141 cgaaaggagc tcgagacgca gtacggtcct gcaagcccga tgctcctcag cgaattggag
    16201 agtcgtcaac tggcgacacc ggcgttcttt gcgggcacca cgctcctcgc agtcccgctt
    16261 ttcgttctca cggcggcgat gttcttcgca tttccgcgtg tgggctttgg gttcttgtcg
    16321 aatctcggtt cgcggactca atcggtggcg ggtttcggag atgacgtcga gctcggcggt
    16381 tttggcacga tccgcgatga ccccacggtg gtcatgcgcg tgaagatgaa gaaccggccc
    16441 gacccggcgc cgcaagcgct aggacttagg ctcagaggca catcgttcga tcgctacagt
    16501 caaggtcgct ggtcacgcac ccaggtcgcc ggggaaccgc tcaagcgtct acaagacgag
    16561 tacatcgtct ttagacctcc gacgcccgag gaccaggcgt acgatatcat cctggatccg
    16621 atggaggagg cggtcttgtt cctcccagag ggaacggtgg cgctgcaagt tccacccctg
    16681 gttcgatccg ggcgcaatca atatcggcga atcgactacg cacctggact ggatctgcgc
    16741 taccgcgggc gtcaggacgg ccccctcacg tactccgctg ccgtggacac ccggcagcgc
    16801 ggctttcccg agcgaatccc taaatcgctc cgcaaacgat acctcgaggt tcctccaaac
    16861 tacgagcggg ttgccgagct tgctcgcgag gtcgtcggag aggagaccaa cccgttcgct
    16921 cgcgctcagc gaatcgaacg ctatctgagg ggcggcggag acttccgcta taccctcgaa
    16981 cagcccgagg tgaaggacaa agacccgctg catgtcttct tgttcgaagc cagagcaggg
    17041 cactgcgagt acttctctac ggcgatggcg atcatgatgc gttcgctcgg actgccggct
    17101 cgcaacgtga ccggtttttt gggagcggac tacaacccct acggggacta ctacgccgtt
    17161 cgcaacggac acgcgcacag ttgggtcgag gtactgatcg acggacgctg gaggacttac
    17221 gatcctacgc ccgcttcagg ccaggtcttc gccgccccgt ccggacttgc agtgaagatt
    17281 cgccagatga tggatgcgat gcgagtccgt tgggccgagt acgtcgtcga gtacaacatt
    17341 cgagaccagg cgagagcctt gcaaggactt gctgagtggt accgctcgct tcgcggaaat
    17401 cgaggtgcca tgcaatcgag cagcgacgac gctttggatg aggatcagca gttcgggtcg
    17461 atcccgttcc gtcccgattg gcgctggttc gtgggcgtga tgagcgcttt tggcgtcgcc
    17521 gtcctcttcg tacgctggag acgcaagcgc aggcgccaga gccgcgcagg caggcaactc
    17581 gatcccgatc gggatcgcgc agtgcggctt tatctctcgc tcgagagtag cctgcgaact
    17641 gcaggccagg cgcgaccggc cgacgtcacg ccgatcgagc acgccgaaga gctcgacagg
    17701 tcaggattcg cggccgccga ggaagtccgc gaggtgaccg ccgcgtatct cgccgcgcgc
    17761 ttcggcgagg atggtcttcc agtccacgac taccaccggt tgcggaaagt gagccgcagt
    17821 attcgcgcca aggcgaagcg cccacctatt gcgtgagaaa ccagccggtc gctacgccga
    17881 tcgctgcgag gaccaggatt cctaagaaga ggaccgcgac cttggaccct ccgggcttca
    17941 tcggctccgg cttttcaatc ggcttcggca ccgctggctg actctcgggg tctgcgacga
    18001 tcgacaccgg ggtcgtaccc ggcggacgaa aggtcatcac cggttgcgag ccctcggagc
    18061 tgagcgaacg ccgtggagga ttgagagatg gccccatcgg aatcctcgca ggacgcggaa
    18121 tactcgcgct ctcgtccttc gtctcgaacc agcccatcgt ggcgccggcc ggggcactgt
    18181 cccgcacttg ctcgattgcg gtttcaactt cgacgtcgcc ggaaaaccag ctactcgggt
    18241 cgacgaagtc ggaggcctcg agagggcggg caccatcgtc atcgagacgc agagcaaacg
    18301 aggtcgaagg gtcgatcggc acgttcgcat cactctcgag tgaagtgaaa cggccgagct
    18361 cctcttgaat catcccgtcg atcatcatcg gttgcggggg aaccgacttc tggcgttcga
    18421 gcgcggcttt gacgaggctt gcgatgtcgt acggcgtcac cttcatctgg tgatgaaaga
    18481 ggtagcttgc cagagcatcg ccgagctcgc gcgcggaccg gtagcgatct gctggtttgc
    18541 gagcaagcgc cttcatcatg atcgcctcga actgcgcgtc cacttcaggg tgggctccca
    18601 cgaggctcgg cacttgcgcg cccgacacgg cctgcactgt ctcgtagtcc gtgtcaccga
    18661 ggaacaatcg ccggccggct agcatttccc agatgcagac cccgagggcg aagatgtccg
    18721 cccgctcgtc gacctcctga cccgtcgcta cctcgggaga gagatagctg aacttgcctt
    18781 tcacgacgcc ggggtcggtc cgctcgagct gcgtgcgcgc cttggccaga ccgaagtcgg
    18841 tgactttcac ctctccacgc ttgctgatca agatgttggg cggcgaaacg tcgcggtgga
    18901 ccaggtctac gggattgcca tcgtcgtcga cgagctcgtg tgcgtaactc aagccgcggc
    18961 aagcttccat cgcaatgtag atcacgtcct tcagagggaa aggctgtcgc ttggcccgca
    19021 gcgtctccat gatcttcttg aggttcgttc catcgacgaa ctccatcacg atgaagaagc
    19081 tgttgtccga cttgccgatg tcgaagacgc tgacgatgtt tgcatgcgag agccgggccc
    19141 cgagccgcgc ctcgtcgaga aacatgccta tgaagttcgt gtgcgaggcg aggtggggca
    19201 gcacgcgctt gatagcaacg cgtttcttga agccctggac gctcgtagct tccccaagga
    19261 aaacctcagc catccccccg gcttcaagtt tttcgatcag gtgatatctc tgatcggtca
    19321 ttccagctcg tccgcccctc tctaaggcgt cattccattg tcgtttgcgg gcgctatcga
    19381 ggtcaaggaa gggatgccat tgacatgctg cctcggcggc gcttgactgc cttcgtgccc
    19441 cgctccgcaa agcccctcag aatcgcccca tcggttcttt cagctgattt tggccgcctt
    19501 gcggccgaag tccaggccgt cgaccaagcc ggcgccgatt ggattcacgt ggacgtcatg
    19561 gacggacgtt tcgtgcccaa catcacgatt ggcccgctga tcgtggaagc gatacgcgaa
    19621 gccacggaaa aaacggtcga tgtgcacctg atgatcgtcg agcccgagaa atatgttcag
    19681 tcgtttgcga gcgcaggcgc cgacatcatc acggtccacg ccgaggcctg cacacacctt
    19741 caccgaacgg ttcaacagat ccgagcccta ggaaaacgcg ccggtgtctc gctgaaccct
    19801 cacacccctc cggactgcct gcgatacatc atgcccgatg tcgacctggt gctcgtgatg
    19861 agcgtcaacc cagggttcgg cggccaaagc ttcattccat cggcgaacga gaagctcgct
    19921 cggattcgca cgatgatcga tgaatccggc tacgacatcg acctcgaggt cgatggaggc
    19981 gtcaagcccg gagtcgctcg cgaagtggtc ggcgccggcg ccgacgtgat cgttgccggg
    20041 agcgccgtat tcaatcgcga cgattacgcg gccgccatcc aagcgattcg aaaggatgcc
    20101 gagtgaacga cgaccctgcg ctgaccaccg tagccgtcga ccgtaaaccc accaagcgca
    20161 agcttcgcaa gagccgtctg gtggtcgaag acgggccgca aaaaggaact cggctcgaca
    20221 tcgcgagcga gcgcatgacc atcggccgaa gcggcatctg tgacctccag ctcaccgatg
    20281 aaggcgcaag cggaacacac tgcgaaatca tcgccaagga agacggcttt cttcttcgag
    20341 acctcgggtc gaccaacggt acctgggttg ctggtgtcag agttcaagag gcgtggttag
    20401 agccaggcat gcccttgagg ctcggcaaca cggtgatccg tttcgagcac gggcccggct
    20461 cgatcgagat cgatctcagt cggcgcgagc agttctatga tctggtcggg cacggcgtgc
    20521 gcatgcggga gattttcgcc gtcctcgaga aagtcgccgc gtcggacctc accgtgctcg
    20581 ttcgtggaga aacgggcacg ggcaaggagc tcgtcgcgag ggcgatccac cgcgcgtcca
    20641 aacgctccca gcgacgtctc gtcgttcagg attgcagcgc cattcccgcc aatctcatcg
    20701 agagcatctt gttcggccat gagcgtggct ccttcacggg tgctgccgat cgcaagcgtg
    20761 gctgcttcga agtcgccgat ggcggcacgc ttttcttgga tgagatcggc gagctcgata
    20821 tcggccttca gccgaagctc ctccgcgtgc ttgaaacacg cgaagtccaa cgggttggtg
    20881 gcacgcggat gatcccggtc gacgtacgtg tggtcgccgc gaccaatcgc gacctcagac
    20941 agatggtcaa cgatggcacc tttcgagagg acctctacta ccgcttgagc gtcgtgcagg
    21001 tcgacttgcc tccgctccga gagcgacccg aagacatccc ggttctcgcc cagcagtttc
    21061 tcgacgagtc gtccaagcga ggcccccaag aagagcgcaa gcacttcacg atcatgcgcg
    21121 acgcgatgac gaaactccag gcctatccat ggccgggcaa cgtgcgcgag ctgaagaaca
    21181 cgatcgagcg cgccgtctcg ctggccgacc ggcccgagct cggaatgcac gacctcttgc
    21241 cagcatcgca aaagacaccg ccggtcgtct ttcctggggg cactgcggag cagttcgtcg
    21301 acaatgggat tccattcaag gacgccaagc agcgcgtcgt cgacgctttc gaagcgcagt
    21361 acctgaaagc ggttctcgac aagcacggcg acaacatcag tcaaagcgcc agagccgcgg
    21421 ggctcacgcg ctaccatctg cgcgagcttg cgaaacgata tggcttgcgt ggtggaacca
    21481 gcggttccga cgaagaagat tgagccttct gcgctgcgcc gcctatcatg gtgatgatga
    21541 ctggggttcg cttcgcgatc gtcgcaatgg tcgcgctcgc ttgggcgcgg cccagtgtcg
    21601 ccgagatcac cgagccggaa gctgccgtcc cggcgccttc ctgggccaag tcgatcgaag
    21661 tggtcaacga tggtgctccc gtgatgctgg gaccgagcag cgaatcacgc cgccgcggca
    21721 ccgtcggagc aggcacgaga ctcggattca cgcgccgcgt cttcggtgaa ggttgctcga
    21781 cgggagtctg gtaccagacc caagacgagc tcttcatctg cgaatcgaac gtcacgctgt
    21841 cgccgcaagc acctggaccc aaggtcgacc tattgaccgg gtccacgtct cgcctgccgc
    21901 agcggtatgc attcattcgc ttcggcggca cgcgcgcgta tgcgcacccg agcgactact
    21961 tccgaaacga ctacctcgag gccttgggga agggctttgg gatcgtcgtg accggcgagc
    22021 aggtctatga gggtgtggag ttcgttcgga cgcgccgcca cctttggatc gagagaggct
    22081 ccgtgcattt cgtcgacggg agcgcgttca tgggggcgag gatcggtcgt gaagaagcgc
    22141 tcgatctcgc atgggtcgcg ctgaggccgg caagtgtcta cgagaagcca cggggtcgcg
    22201 tcatcaagcg cctcccaaga cacagcctcg tacacgtcga gtcgagcaag ggtgcgtgga
    22261 gcaagctcgt cggggaaggg tggatgaagt ccgccgagct cgtgagtcca aaacggaggg
    22321 atccgcccga gggcgtagga cccaccgagc gttggattga tatcgacgtc tcgcaacaag
    22381 tcctcgtcgc atacgaaggg gcggagcccc gatttgccac gctcgtctcg accggccgca
    22441 acaccaagca atcggaaacc ccgcttggcg tctttcagat ttgggtgaag ctcgagtact
    22501 ccgacatgaa cgacatcgaa cggaccgacg tcgacaagaa ctattcgatc caggacgttc
    22561 cctgggtcca gttcttcaaa ggctcctacg gcttccacgc ggcgttctgg cacgacgact
    22621 tcggccgccg ccgaagccat ggctgcatca atctctctcc gaaggatgcg cgctacctct
    22681 tccagttcac gggaccggtt ttgcccccgg gttggaacgc catcctttcc atgcccgaag
    22741 atcggcccac cacggtcaga gtccgctaaa gacggcagga ctacggtctt ccgacggact
    22801 cttcttcgtc agccttcgag cgcttccttg gcgttcagga gcgaagcgtc caggccgcgc
    22861 ttgttacgcc tgagcgtgag ctgctcggct agcttcccga gcactggagt agggatcgag
    22921 tactcaagct tgatcgtcag cttcgttccg cgatcgcttg gctcggtgat gttcgtccat
    22981 atgctctcga tcataccgat gctctggtaa gccgcgcact cgtcttggac gtagttgacc
    23041 gcgacgctct gtccgcgaaa cagaagcccc accatcttga aggtccactc gtactgcaga
    23101 ccttcgccgg tcccgaccac gttgcggacc tcgatcatgc cgttgaccca gtcgggaaac
    23161 gtcgcgggct cgttcacata cgcgaacacg acgccaatgg gcgcctcaat gagaatgctg
    23221 ttctcgtagg aaggcaactg gcccttttac tttcccctgg ggaggttggt gaaacctaca
    23281 aaccgcccga ggctctgtcc atccgtaatt atttagtccg tattaggtca cgcattttct
    23341 gcgcttctga cggcggtttc cgggaattga ttgcccagcc cagcgcgcgg gaaccaatcg
    23401 cgatggcgca aatcgttccg caactcccat tgcggtcgac cgacaacgct gcgtggtgtt
    23461 cgcagagtac atgctggtga ctgcggtgat cgttctagcg gtcggactcg cgctttatgg
    23521 gctcggcgtg ccactcgtgc aggcgttttt cctcacgaag ctcctcatcc tcttaccgtt
    23581 tccataagaa aggaaggcac ccatgaacga cgaggctcaa atcaagaggt ccctcaggaa
    23641 tgaccagcac gggctgacca cagttgagta catcatcgta ctcggtctga tagccgtcgt
    23701 tggaatcgcc gcctggcaga agtttggcga aaccctcatt ggcgaagtcg aggcagccga
    23761 cggctatatc tcggacgtgg ttcagtagcg gtctgagcga tgaacgcggt agctacaatc
    23821 gcctcaggct tcgcactcgg tgtcgcgatc atcggcgcta tccgcgactg gcgaagcggc
    23881 gagataccga actggctgac tcttccgccg ctcctgatcg ccccgctcgc gtacggtctc
    23941 gcaatcgggc ccgaacaagc cgcgcgcagc tttgccgctg catgcttcaa cgcgctcgtc
    24001 ccatatctac ttttccgtcg cgggggaatg ggcggcggag acgtcaagat cttcggggcg
    24061 ctcggggcgg ttacgggttt cgacctattt ttggggcttg agattcagct tgcagccttg
    24121 ttcatcgcga tgctgtgtgc actcggagca ctggcctgga gaggccaact gctgagcacc
    24181 ctagcaagcg ctctcgctca gatttcaccc ttgctgctgc gggcgaggca tcgcccccag
    24241 ctttcggaca acttgtccgc acccattcga atgggcgcac ccattctgat tgcgacagga
    24301 atctatgttg caccacgcct attgttcgca tggggtcagt cttgacacgc cgactcgccg
    24361 cgacgcctgg cattcgcgag gacaagcgcg gcgtcgcgtt tctagaattc ctcatcgcgt
    24421 tcgtgcccct atggacgttc tttctcgccg tggtccagct cgcattcatc gcgtacgcgg
    24481 acctcttggt gaagcactct gcggatgccg ccgcccgctc tgcgatcgtc gttctaccgg
    24541 acgacccaaa cgaatacgga ggcgaacccg agatgagcat cgaccgcaat ccgttcacct
    24601 actctgacat ctccagggcc ctcggtggcg ctggcgagat gatcggcgtg agctccgatc
    24661 cgaaccgcat tgcagcggcg ttttcccaac aggtcgtgtc gaatctcggc agatcgcggc
    24721 tcaacacgat tcggctggcc gctcacgttc cgctgatgtc cttgaccccg gccgacgttg
    24781 gccgcgaccc gcaaccgagt atcgcgaaga gcatcggtac tgagcggaag ctcctgagcg
    24841 cgctctacta tcagccattc gcgcttgccg taacgtttcc aggattggaa ggcggtgcgg
    24901 cgctgggtcc ggaaatcaca gttcgcgtga cctatgccta ccagtgcacc gtgccacttg
    24961 cacgccaaat tctctgtaag gtctttgacg agctcgaggg gaaatccgaa ttcgccgacg
    25021 ccttcttccc tcttgctcga gctttcgtag gagggcgctt ccgcacactc cagcacgaaa
    25081 cgacgctcat gattcatgac gcaccgtacg tctatcgaac ggagggcgtt tgatgttcac
    25141 agggaccaag agcctgtacc aggacgcgcg aggctcgatg atgctgatgt ccttgttcat
    25201 ggcgctcttc gccatcggtg cgctctacta cgttctcggt gtcggggatg caatcctgta
    25261 ccggcggctg atgcaagacg gcgccgacgc aggcgcgcac gctgcgtctg tcatgggcgc
    25321 aaaaggaatg aatctgcatt ccctcctcaa tgtcgtcatg gccgtcacta ccgggatcct
    25381 gctcgtgatt cggagtgtcg aggtcctctt ggagatcact ctcggcgttc tttacggcct
    25441 gttggcgacg atcgtcctca gtgccaaagc aagtgcactc atcggagtgt tgaccccggt
    25501 cgaaagcacg atcgaaaggg tcggcgatgc agtcgagcag ttcgtgcaag tcgcacacga
    25561 cgctctggac gtagcgcatc acgccgttca gcgcggctat ccgctcctgg cgcaggtccg
    25621 cgcggtcgac gccatggcgt ttcaggaagt ctacgaccca ccagtagcgg gggggtttgt
    25681 cgttccgctc ttcgggcccc gacttcccga aggcggtctg ggtctacccg tggagaaggc
    25741 ggagatcggg gcgctctgcg atcgcgtcgc tgatgggctc cagaacaggc tcggcaacgt
    25801 gagatccaag gttccaaaat ggcttctcaa gttcctcggt ggcgccgttg gcggcgcgct
    25861 gcgattggga aaacgcagaa cctgtgccga cgacatcgta gagtcaccgc gacgtgtaat
    25921 cgagacacga gctgatggaa gcgtcgtttg gctagggcac gaggagtttc agtacagggc
    25981 atacgatatc ggaagagacc cgaacgacaa tcactggcgg cacggcgagc ttggcatccg
    26041 tgccgcgcaa gggggcgcgg agggcggcca caacgggccg tacgctgctc acgtgatagg
    26101 acgaatcgga ttcgcccagt ccgagtacta tttcgacgga gtcgaagaca agtccgagtg
    26161 gctctggaag cagcgctgga gatcccgcct acgccgcttt cgcatctcgc gcgattggat
    26221 tccgagcacg atcttgggcg cgtgctccgg caccaggggg cgcggttcaa tccacggtct
    26281 gagtcgtatt tgcgatctgg cgcgcgactt ttccatcaac gccgtatcgg cccattagga
    26341 ccccgatggc cgaatcgatc ttgcaattag ttcgagacgt caaggggacc gcgctcgcgg
    26401 aggctctcat tagcctccca attctcgctg cggtcttggc cggcgtggtt gccttcaacg
    26461 ggatgtacat cgcgaagctg gaagccaaag gatgggccag gcgcatggct tggctacaag
    26521 cagagtccgg tgactgcccg gcgcagagtt gccggagccc cgaatgcggg cgcatcgagg
    26581 atcagattcg ctccgaggga ctcaatcagc cccttgaagt gcagaacagc tcgttctccc
    26641 tgagcgcttt cttgggtgac ttgggtcact tctttctcgg aaagatcaca aacgggattg
    26701 gcattgcaga cgcggcgatg ccctccaccg tgcgtcaaga cctcggtcat caacgcggtg
    26761 tgaccacgtt gctctgcaac actcgatcgc ggcatacgga tacgggggac agcgtgctcg
    26821 agcatgtatg tcggacgggt ctaagaacga cggagtacgc cagtgaagtc tgcaagtaga
    26881 gtccacacac ccgtccgcct cgcagttcgt tggcccttct acctcttggc gacgctccgt
    26941 ctcggggtct acctttcggc cgtttcgttg ttgagcgcct tgatgttcct gcgagccgtg
    27001 cgtgctgatg tggagcagct aatttgggac agcggtgcga gtctcatgca acaccccgaa
    27061 gcgaccgctg ggatcagccg acgattggag ctcaatggcg ccacaatctc attcaggacg
    27121 caaaccatcg cggcttccct ggagtcagtc ctcgatcact acgaatcctt atgcgaaagc
    27181 agggacgcgc acctcagtgc agcactctcc aaacgcctga acgagcgttc tgccgctcgg
    27241 aagaccccgt cgatgctgcg tgccgtcacg acgacgacgg cacgcaatgc tgattcggga
    27301 tacgtcgcct gcctggactt agggtctgaa ccgcgagact tggttgcact cgccgacggg
    27361 ttcgtccgct tcgccgcgac gggcaatctc cgcgaagcag gcgttctgcg gtacgtgttc
    27421 gcgcgtcgcg cgtcgtcgga gcccggatcg aagacattct ttctcacgat gtctgccgaa
    27481 tcggagctca acctgcacaa gatcgttccc gatggggact ccgatgccgc gggacgagat
    27541 ctggtcggcg ttccacgccc gctccgatcg cagcgtctgc tttccgcatg ggaagtggat
    27601 caacccagtg ggctcgttct gtatcgggcg tcagggacga cacccgagca gatcgagtcg
    27661 ttctatcgga ccgagttgct gcgcctcggc tggaagatca tcgaacgaaa tcgaggcgag
    27721 tcgatccaga tcgacgggct tcggatgttg tcagccgagc agcgcgatcg cgtggtcacc
    27781 atcctctcgc gtcccggaga gcaagaaacg gcgctggtga cgattcttgg ttcggagccc
    27841 acacgatgaa tcgcaatgca atgttcgcct ccgtcgcggt ggcctccgtc ggtgtaatgc
    27901 tccttggcgt gtacatccgg cagtttcagc gcgagacctc gggaggtgaa ccgatcgctc
    27961 tcctcgcgat gcggcaggac gtcgccgctg gcgttccact caacgagcaa atgttggtag
    28021 tgcgaagact gccggagagt tatgtagagg cccgccaggt gctcgcgtcc gatctgcccc
    28081 gagttttggg ggttcgcgca tccattcctc tcgaagccaa tcagaccctg ctgtggacgg
    28141 acctcgcggg cactctgcag catcgagcgg cgctctcaac tcgtattccg agaggaatgc
    28201 gtgccatggc cgtcactggc gccggcagag gcgatctcgg tgagctcatc cggcccggcg
    28261 atcgcgtgga cgtcttgctc accaagacga aaccggggcc cgaaaccaac gtcgtgacgg
    28321 ttccccttct tcaaaacgtt ctcgtactcg ccgtcggcaa agagttcgat ccgatgagga
    28381 aggaggccca ttcccgagga tcagattcag tcactctctt gctgaccgtc gaccaggcga
    28441 gcttgcttgc tcaggcgcgc cagaactcca cattgagtct agcacttcgg aacgagaacg
    28501 atctcgcgat caacgaagca cttccagaaa ccaacgactc ggacatcctc gagctcgaga
    28561 agcgagcgag tcggcagcgt cgcctgagaa tcgagaaggt cgactagcgt gtgcttttcg
    28621 tcaaaagcga tccttgtttt cggactcgta ggcctcttgg ctacagtgga gatgcgtgcc
    28681 accgcggaga ttggaagtgc gcgtgaattg gttctgcggg tcggcgagca gcgcagcctc
    28741 cccgccgacg gcattcgaag ctactcggag ggagtgcccg gcatcgcgga gatccgagtc
    28801 acccgggacc agagtcgatt cgtgatcgtg ggaaaggcag agggcagtac ctcgcttctg
    28861 ctcattcgga gtgacggcac acaagttcaa tacacgattc gcgtggttcc cgccgatgcc
    28921 aacaaagggt tcggcgagcg ggagaacatc cgcttggatc tttacttcgt catgatccag
    28981 gatcgctata gccaccagat tggactcaac tggccgggca acgttggaag cacgctcacg
    29041 ccatccgagg ccaccttcgc aatttcgggc gggtctcagc aggttacgca aagcgcgttt
    29101 cagattcttc ctcagacgtt tttgccaagc ttcgatctcg ctcaggccaa aggctgggcg
    29161 aaaatgtatc ggcaggcgac gctcgtcacc gccaacggga ccgaggccgt cttcacatct
    29221 ggtggcgagt tcaacgtcca ggtgcagaac aacctcacag tcaacgtgca ggcgatcgag
    29281 ttcggcacga aggtttccgt gcttcccaag tacgattcca gaaccggacg gatagagctc
    29341 gaaatcgttg cagacgtatc agacttggat gccgaaagag gaattcgagg gatcccgggt
    29401 cgcatcacct cgcacgtcga aacactggtg aacctcgagc tcggtcagtc ggtctcgctg
    29461 gccggcatca gggctcgtac ggaacgaaga aagaaggacg gtattccggg actggcgctc
    29521 gtccccttca tcggggccct cttcggcagt cacggtcgcg aaacagaaga caacgaatcg
    29581 tatatgttcg tggtgccttc ggtggtcgag ccggtgagcc tcagtcaacg caatcgagtc
    29641 gaagaagccc tacgtgcgta cgagggcttc caaggaggag tcgatgcgca tcagctcaag
    29701 gaccagccca gggtcggaac cgagcgcccc tcatcaaagg gacgcaggac tgcgcaatga
    29761 cggccggatt ctcggtgaat gttcgcatgc ccgatggctc ggagcgcaac ttcgatgtca
    29821 cgggaacgga ctctctcacc gtcggcaggg acccgtcatg ccacgtcgtc ctctcctcgc
    29881 ccgccgtgag tcgctttcac ctgcaggttc tcgacggcga agatggtccg cggattgtcg
    29941 atcagtctgc caacgggacg ctcctcggaa gcgaaagagt cggtgccact ggcgctccac
    30001 tcatgcccgg tggccagttg cgcattggac cgtatcttct gcgaatagca gtcctacggg
    30061 cagcctcgat cgacggagcc gcatctcccg agctacggag gacgatccat cgctccctat
    30121 tggatcatct cgaccttgcg gctcttgacg agatcgacat gcaagcagcg gcgctgcggc
    30181 cacgcgtgat cgaggccctg aagcgaatcg tatcgaccct tgacccgagc gtctgtccgg
    30241 agagcgagaa ggagagattg gtcgcggaga tgaccaatga ggcgctcggt ctcgggcctt
    30301 tgcaggaact gctggacgac gattcggtgt cggagatcat ggtggtcgac ccaaacacga
    30361 tctacgtcga gaagcatgga aagattcggc tcacgcctct tcgtttcacc gatgacgaat
    30421 cctgccgtgc ggcaatcgaa cggatcgtga ccccactggg ccgccgcatc gatgaaagca
    30481 ctccgctcgt cgatgcgcgc ctcaaagacg gatcgcgcgt gaacgccatc attcctccac
    30541 tcgcgattcg tggcccctgc atcacaattc gcaagtttgc ccgacgaccg cttctgatgg
    30601 atcagctggt cgacctcggg agcctgagcc cccagatggc gcgatttcta cggaggtgcg
    30661 tccgcgccaa gaagaatttg atcatttcgg gcggaacagg aagcggaaag accacactgc
    30721 tcaacgttct ctcgaccgag atcgatccga gcgagcgtat cgtcaccatc gaggacgctg
    30781 cggagcttcg tctagatcag ccccacgtgg tgtctctcga atccaaaccg cgcaatctcg
    30841 aaggagtcgg cggctatgaa atacgcgacc tggtcaagaa cgcactccgt atgcgtccgg
    30901 atcgaatcat cgtcggggaa tgccgtgctg gagaggccat cgatatgctt caggcgatga
    30961 ataccggcca cgaaggatcc atgaccacga cccacgcgaa cagcgcaggt gaagctctca
    31021 agcgcatcga gacgctttgt atgatggccg gtttggacct gtcaccacgc accatccgcg
    31081 aacagatagc gagcagcatt cacatcgtag tccagcaaag caggttttcc gatggcacgc
    31141 gccggctcac atccgttacg gaggtaggcc ccttgggccc cgatggtgag ctcgtcctgc
    31201 atgacatctt cgcctttcaa cgaaccggaa cgagcgcgag cggggaagtc gtgggcgtgc
    31261 accgaaccac gggttacgtg ccgagcttcc tcgacgagtt catcgcgcaa ggcctcatcg
    31321 acggcgggga ttacttatga ggctcgaatc gctccccctc ctcattctcg cggctgcgtg
    31381 tctagtcgtg ggttgcggat tgatcactta ctccgcgctt tccctggagg ataccccggt
    31441 gcggcgcctg ttcctgcgtt atgcaggtcg tttggaccgc cattcaggtt ttctcctgat
    31501 gtcgcagagt ggacgggaga tcgcccgaat gcaggtcatc gcaggcgctc cggtggccgc
    31561 gttctcgatc gtcgcgcgca gccccggact ggcgcttgtc gccgtcgcga ttttcgccat
    31621 tcctccattc gtcctctgga agcggcacga agcgcgagtc gctcagctcg agaggcagct
    31681 cggcacctgg ctgctcatgc taaccaacgc gctgaaagcg acctcgtcgg tgggagaggc
    31741 cattgcctcg acggtagcgt tggtccctaa gccgttcagt gaagaggtcg accttctggt
    31801 caaagagatt cgcctcgggg ctcccttgga tcgggcggtc agtgcgcttg ctcgccgcat
    31861 caacagcaca gtagtgtccg gtgctctttc aaccatcatc gtcgcacggc gaacaggggg
    31921 cgacctgccg aagaccttgg agcgtgcatc cgcggcgttg cgcgaatcgg ctcggctaga
    31981 aggggtcctt cgaactaaga cggcagaagg gagaggacaa gtcacggttc tcgcgtcggt
    32041 tcctttcgtc ctttgcgtga tcattgcctg gctcgatcga agctggtttg acccgatgat
    32101 ccatcatccc atcggcaaag gcattctgct gagctgcgcg ggcgcgtggg tgtttgccac
    32161 cggttgggcc taccacatca tcaaggctga tttatgacag aacgcctcaa catcttgttg
    32221 gcgttgtccg ttatcacgat ctttggaggc actgccggcg tctgcctcca gttggcgaag
    32281 aggcccgctg tcccgcgtcc gagcttaggc gcccgagggg tcaaacgggc acgggccctt
    32341 cggcatagcg cgctcttcgc tctcaccgag ccgcccctac gcgctctcgc cgcctggatc
    32401 gcaattcttc caattgcagt c
//
LOCUS       DASRZF010000090         2318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2470810, whole genome shotgun sequence.
ACCESSION   DASRZF010000090 DASRZF010000000
VERSION     DASRZF010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2470810"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..1818
                     /locus_tag="VFG22_04060"
     CDS             205..1818
                     /locus_tag="VFG22_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415444.1"
                     /translation="MTAARSPAGPRRIGPPLTILATALLAMLFLYAPVRDFGFVWDDV
                     AEVQRNPLLHGALGPGLLASNHEHMGVRRADWLLPSHESLRPLRYLSYRLDAVLHGLN
                     PGAMHLHNLLLAWVGLLVAYFVARRWGLGGAAAALAALIFGLHPLQVEPTAYISARSD
                     LLGGVLGLLSVGLGLSALQRLQHFYFWLAASALAFLLALSAKEATVLLPFTVVVQGFV
                     LLRRDDALKWDRLAWVGATYALTAAVWFGLRSSYVRSSGSLSPIDWAHGFGTGLLQYL
                     WIFLKPIDCSVVRPFAPGSWWALAACLLVTFAGLAWLWMRPRGAASRYVPLLLMSIFA
                     LLPSLVAASVTGIASDRYAYTACFPFAVAFVQLGSDLRRRFPVTRAAIFALGGAWLLA
                     LLFIVTTLVPAWRDPIALYGHALRIEPSSAAAHYGLGSAVMGTAGCEIAEPLFLHAIG
                     LDPNYLRAYTNLSVCEMRRGALDEAAATLERALELGGGLNPKVWANLAEVRLGQGNTR
                     DACAHLERAMALDPGYAFAEELLAKHCQR"
     gene            1941..2201
                     /locus_tag="VFG22_04065"
     CDS             1941..2201
                     /locus_tag="VFG22_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415445.1"
                     /translation="MEDLQVRGKRVRIEAWEPGTVQEMKAVPLDQVDRYGIEGAPLAG
                     NLPAAFYEHAVICMAWDRIDRCMMYEYVEFPIDVYLQDRNAS"
BASE COUNT          388 a          760 c          694 g          476 t
ORIGIN      
        1 tcgaccagcc ctggaagcca atcgggcatc gtgaggaagt cgtccgcata tgcgaagact
       61 tcgttcactg gcgctttgat cacgatacta ttctcatatt ggatcatacg aagacgccgc
      121 cccctctagc ctgaacgtag aatagtgcca ccagatcccg aagaacaacc gacccgacat
      181 cgtcattcca gattggccgc aagggtgacc gcggcgcgca gcccggccgg acctcgacgc
      241 atcgggccgc cgctgaccat cctagccacg gcgttgctcg caatgctctt cctctatgcg
      301 ccggtccgtg acttcggctt cgtctgggac gacgtggcgg aggtgcagcg caatcccctt
      361 cttcacgggg ctctaggacc cggcctactc gccagcaacc acgagcacat gggggtgcgc
      421 cgcgcggact ggttgcttcc aagccacgaa agcctgcggc cgcttcgcta cctttcgtac
      481 aggctggacg ccgtcctcca cggtttgaac ccaggcgcga tgcacctgca caacctgctg
      541 cttgcctggg tcgggttgct ggtcgcctac ttcgttgcgc gtcgctgggg tctcgggggc
      601 gcggccgcgg cgctcgctgc gctgatcttt gggctgcatc cgctgcaagt ggagcccacg
      661 gcctacatct ccgcgcgaag cgacctcctc ggtggagtgc tcgggctctt gtcggtaggc
      721 ctcggactca gcgctctgca gcggctccag cacttctact tctggctagc cgcctcggcg
      781 ctggcattcc tcctggctct gtccgcgaag gaggccaccg ttctgcttcc gtttaccgtg
      841 gtcgtacagg ggttcgtgct cttgcggcgc gacgatgccc tgaaatggga taggctcgcc
      901 tgggttggcg ccacctacgc gctgaccgcg gcagtctggt tcggcctgcg ctcttcttac
      961 gtgcggagct ccggatccct atcgccgatc gactgggccc acggcttcgg gaccggcctg
     1021 cttcagtatc tctggatctt cctcaagccc atcgactgtt ctgtggtgcg gccattcgct
     1081 ccaggcagtt ggtgggcgct cgcagcgtgc ctcttggtca cgttcgctgg actcgcctgg
     1141 ctctggatgc gaccccgtgg agctgcgagc cgctacgttc ccttgctgct catgagcatc
     1201 ttcgccctgc tgccctcact ggtcgccgcc tcggtcaccg gaatcgccag cgaccgttac
     1261 gcatacacgg cctgctttcc cttcgccgta gcattcgtgc agctcgggag cgatttgcgc
     1321 cgacgatttc ccgtaacgcg cgccgcgatc ttcgcgctcg gtggagcctg gctgctggca
     1381 ctgctgttca tcgtgaccac cttggtgccc gcctggcgcg accccatcgc actttacgga
     1441 catgcgctgc gaatcgaacc gagctccgca gccgcacact acggtctcgg ttcggcggtc
     1501 atgggaacgg ctggctgcga aatcgccgaa ccactatttt tgcacgctat cgggctcgat
     1561 cccaactacc tacgcgcgta caccaacctc tcggtctgcg agatgcggcg cggtgcgctc
     1621 gatgaagccg ctgcaaccct cgagcgtgcg ctagagcttg gcggcggcct gaatcccaaa
     1681 gtctgggcca acctcgctga agtcaggctg ggccagggca acacccgcga tgcctgcgca
     1741 cacctcgaac gcgcgatggc tctcgacccg ggctacgcgt tcgccgagga gctgctggcg
     1801 aagcattgcc agcgctgaca actatcggaa gagcgtcggc tcctcgtctg atcagcctga
     1861 atgcgccgca cgtcggcctg agcttcgtca tggtagagcg agccctggct ggagctttgg
     1921 ccaatactct tcacgcatac gtggaagacc tgcaagtgcg gggcaaacgg gttcgcatcg
     1981 aggcctggga gcctggcacg gtccaggaga tgaaagccgt cccgctcgat caagtcgatc
     2041 gatacggtat cgaaggcgcg cccctggctg gcaatctacc agccgccttc tatgagcacg
     2101 cggtcatctg catggcttgg gacagaatcg atcgttgcat gatgtacgag tacgtagagt
     2161 tcccaattga cgtgtatctc caagatcgaa atgcaagttg aaccgactcc gacggggatg
     2221 gaagatgcga ctcgcacgac gagctagatt tggagatctt cgtctcacgc ttgcagacgc
     2281 ctccagaaaa gcaaaagtgt gagaagagcg agctcttt
//
LOCUS       DASRZF010000091         5519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1442156, whole genome shotgun sequence.
ACCESSION   DASRZF010000091 DASRZF010000000
VERSION     DASRZF010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 5519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5519
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442156"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VFG22_04070"
     CDS             <1..255
                     /locus_tag="VFG22_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415446.1"
                     /translation="TRGSKAFEKELYMGQELRQNASNYVILTNPEQSAGVLGQRKNAF
                     ASYRRDLADIGRLMEATLETQGTLEKGLGSVAGLEEIASF"
     gene            382..1263
                     /locus_tag="VFG22_04075"
     CDS             382..1263
                     /locus_tag="VFG22_04075"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET6415447.1"
                     /translation="MPLGEFAIGRSSSCNLALADGLVSRKHAVLHVGPDGVVVEDLGS
                     RNGVAVNGVRINAPRRLAHMDRVYIGSQELVLIDAAKLRDGTKETEGYIVCASCGAIN
                     GTAKRRCGECGVRLVSSAGETVASTNRGVDSSSHAWGAEDTRTARALDVIAGIASKSI
                     AMGRFEEAERILIPHLDTLLERALAHRPLSDSEKDNPDLLFAGATIYALQLAQGLRST
                     KWIDWVFRMHTASERLMEADTIETLHNLVRNNKYSNTAYLRAYLSVIHNRAADYGASE
                     RFLLRRLEGLEQVVSTR"
     gene            complement(1268..2056)
                     /locus_tag="VFG22_04080"
     CDS             complement(1268..2056)
                     /locus_tag="VFG22_04080"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET6415448.1"
                     /translation="MRYRLRLHLQEIDLLGDEVVIGRGSMGQITIEDPMLSRRHIRID
                     LSGSEPLLEDLRSRNGTQLNGRPLIGPAILRDGDRIRIGTQELVFLVPNVVRTQQRTT
                     SGLSFCVGCAVPYPTSSPQCPHCGAIPTAHGQHAPLRDVAASGWTFHLLSQVVTRALD
                     QGRLADAERMMSRGVSEMEQQIEAGVEVEPSRILGVAAAAARLGCALRSALWLETAAR
                     LHGHAGSSPSREVLAWIDELPTDPLLESAKTALRHSGSDTRRAG"
     gene            complement(2133..2921)
                     /locus_tag="VFG22_04085"
     CDS             complement(2133..2921)
                     /locus_tag="VFG22_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415449.1"
                     /translation="MALGAGGYAETSSASNTGPSALEQSMGEIQWGWTPKQVYRHLKK
                     NIESSYAEPISKTTDAIEEDRLRHRMEEETSEIRDSYFEFDGTASAYDSGYLKGEFTH
                     QNGESLLRVRTKTTQDYFFFINDRLWKRYRAFDASVFEGASFDEFGKALQQRYGKAQR
                     KNGVLVPGAEPTQWYEWKEPRILARAVDNNEFYGFYCLILEDPKTVARLATLRKNQPK
                     EAVATDTLVDMVADESNGDQNADIADRITGEIRRHDSPEVDEKP"
     gene            complement(3032..3859)
                     /locus_tag="VFG22_04090"
     CDS             complement(3032..3859)
                     /locus_tag="VFG22_04090"
                     /inference="COORDINATES: protein motif:HMM:NF018917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HET6415450.1"
                     /translation="MKGVARGAGAVARSPVGFARGLSYAFRGMRFVYFQHPKLARYWV
                     FPILITGLALAAVFYGAGSFYDDVGSMVWSLFPESWNEASGWVGGLLSALRWLIELVA
                     GLLIAVLGLVLVLLLSSVVAAPFNDALSEAVEHMLTGRAAPPFSVSHMLADVARTVRL
                     EILKVILYLGVVGPMFLASIFLPGIGQLVSLVGFVLTAVYLGVDYIDWPAARRDWSVR
                     DRLSFARAELPAVAGFGTGVWILLFVPLVNLFFMPAAVAGGTMLFLSLERPQLPKQS"
     gene            3977..4774
                     /locus_tag="VFG22_04095"
     CDS             3977..4774
                     /locus_tag="VFG22_04095"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HET6415451.1"
                     /translation="MTHGIDAVTAAILGALQGLTEFLPVSSSGHIAIGSQFFGIQENS
                     LALVILLHLGTLLATLVLFRQDVSALAAEALMAVREPRRLGSTPEGRMLVSLALATLV
                     TGMLGFLLRKYAEAFAEDSSLVGYGFLISAAFLIASSVAVGTRSDVSWRQAIVIGMAQ
                     GLAVLPGVSRSGLTITVGMLLGIQGNAAFRFSFLVSLPAIVAAAVYEASGADGLGSLG
                     AQAWMGGLTAFVTGYLALVILRQVILVGRIWMFSIYLLPLGLFLVTR"
     gene            4790..>5519
                     /locus_tag="VFG22_04100"
     CDS             4790..>5519
                     /locus_tag="VFG22_04100"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HET6415452.1"
                     /translation="MNKNEKAYAVIMAGGSGTRFWPLSRSTRPKQFIALGPDDRSLLR
                     ATAERVWDLLPPERTFVVTSELLRDQVANELPELDPSRILAEPVGRNTAPCIGWAATH
                     VKRLDADAIMAVLPADHYIRDTEAYLETLRRGLEAATHGDYVTIGIRPHRAETGYGYI
                     EVGAEIDPGVFRARRFVEKPNRQRAEQFVASGGFLWNSGMFFFLASRILDAIDQHLPG
                     LGEELKRYDEAAKAGNEDRAVQATY"
BASE COUNT         1090 a         1718 c         1676 g         1035 t
ORIGIN      
        1 acccgcggca gtaaagcctt cgaaaaagag ctctacatgg gccaggaact gcgccaaaac
       61 gccagcaact acgtgattct aacgaatccg gaacagtccg ccggcgttct cggtcaacgc
      121 aaaaatgcgt ttgcctccta ccgccgagac ctggccgaca ttgggcgcct gatggaagcg
      181 actctggaga cccagggcac cctcgaaaag ggcctgggct cggtggctgg gctcgaagag
      241 atcgcgagct tctgaactga attcccctcg agggatcgag ggttgcgggt gcagatcttt
      301 cgacgcgatc gatcctcaac tgctattcta gcgactgggg tgccgcgctt tagactgcga
      361 tatcagacga ccaatctgga gatgcctctc ggggagtttg ccattggtcg aagctcgtca
      421 tgcaatctcg cgctggcgga tggcttggtg tctcggaagc acgcggtgtt gcacgtcgga
      481 cccgatggag tggtcgtaga agacctcggg agccgcaacg gtgtcgcagt caatggtgtg
      541 cgaatcaacg cgccgcgccg actagcgcac atggaccgtg tgtacatcgg ttcgcaagag
      601 ctggtgctga tcgacgctgc caagctgcgc gatggcacga aggagacaga gggctatatc
      661 gtttgcgctt cctgcggagc gatcaacgga actgcgaagc ggcgttgcgg cgaatgtggc
      721 gtgcggctcg tctcgtcggc cggtgagact gtcgcgagca cgaaccgcgg cgtggactcg
      781 tcgtcccatg cgtggggcgc ggaagacacg cgaaccgcac gcgcgctaga tgtgatcgcg
      841 ggaatcgcga gcaagtcgat cgcgatgggc cgtttcgagg aagccgagcg cattctcatt
      901 cctcatctcg atacgctcct cgagcgggca ctcgcacacc gacctctgag cgactccgag
      961 aaggacaatc ccgacctctt gttcgccggc gcaaccatct acgcgcttca attggctcag
     1021 gggctgcgaa gcacgaaatg gatcgactgg gtgtttcgga tgcacacggc atccgagcgg
     1081 ctgatggaag cggataccat cgaaactctg cacaacctgg tccggaacaa caagtactcc
     1141 aacaccgcat atcttcgcgc atacctcagc gtgatccaca accgcgcagc ggattatggg
     1201 gcttccgaga ggttcctgtt gcggcgcctc gaaggcctgg agcaagtcgt ttctacccgc
     1261 taacgcgcta acccgcgcgt cgcgtgtcag agcccgaatg gcgaagcgcc gtcttcgcac
     1321 tctccagcag ggggtccgtt ggcaactcat cgatccaggc gagcacttcg cgagatggtg
     1381 aagaaccagc atggccgtgc aggcgcgccg ccgtctcgag ccagagcgcg cttcgtagcg
     1441 cacagcccag acgcgccgca gctgccgcca cgccgagaat ccgactcggc tccacctcca
     1501 cgccagcctc gatctgttgc tccatttcag aaacgccgcg ggacatcatg cgctcggcgt
     1561 ccgccagccg accttgatcg agcgcacggg ttaccacttg ggagagcaaa tggaacgtcc
     1621 agcctgacgc cgcaacgtcg cggagcggcg cgtgttgacc atgggcggtc ggaatagcgc
     1681 cgcagtgtgg gcactgaggg ctcgaagtcg gatacggaac cgcgcatccc acgcagaagc
     1741 taagcccgga cgtagtgcgt tgctgcgtgc gcacgacatt gggaacgagg aacaccaact
     1801 cctgggtgcc gatgcggatt cggtccccgt ctcgaaggat cgcggggccg atcaatggcc
     1861 gaccattgag ctgggtgccg ttccggctcc gcagatcttc gagcaacggc tccgatccgg
     1921 agaggtcgat ccgaatgtgg cgcctcgaca gcatggggtc ttcgatcgtg atctgaccca
     1981 tcgatccgcg accgatcacg acctcgtcgc caagcaggtc gatctcttgt agatgaagcc
     2041 gtagacgata ccgcaagccc gaacccctca ggacccatca gcgtactggg cggaaagggg
     2101 cagtgtaaat cctacccgaa cctacgttgg agctatggct tctcgtcgac ctcgggggag
     2161 tcgtggcgcc gaatctcacc cgtgattctg tcggcgatgt cggcgttctg atcgccgttg
     2221 ctctcgtccg cgaccatgtc gaccaaggtg tcggtggcga cggcttcttt cggttggttc
     2281 ttgcgaagcg tggcgagccg ggcgaccgtc ttcgggtcct ctaggatgag gcagtagaaa
     2341 ccgtagaact cattgttatc tacggcccgc gcgaggattc gaggctcctt ccactcgtac
     2401 cactgggttg gctccgcacc cggcacgagg actccgttct tcctctgggc cttgccgtag
     2461 cgctgctgaa gcgccttgcc aaactcatcg aagcttgcgc cttcgaacac ggaggcatcg
     2521 aatgctcggt atcgcttcca gagacggtcg ttgatgaaga agaaatagtc ctgggttgtc
     2581 ttggtccgca cccgaagtaa ggattcgccg ttttggtgag tgaactcacc tttgagatag
     2641 ccggaatcgt acgcgctcgc cgtgccgtcg aactcgaagt acgagtcacg aatctcgctg
     2701 gtttcctctt ccatcctgtg gcgaaggcgg tcctcttcga tggcatcggt ggtcttcgaa
     2761 atcggctccg cataggagct ctcgatgttc ttcttgagat gccgatagac ctgctttggc
     2821 gtccaacccc actgaatctc gcccatcgac tgctcgagcg cgcttggacc cgtgttgctg
     2881 gcgctgctcg tttcggcata gccgccagcc ccgagagcca taccaagagc caaaacgatc
     2941 gccgcgtaag atgaacgtcg cataaccaat ccccttgggc tcaagttagc acccagcgaa
     3001 ccttccatac aaaaaccgct ctgccccatt tctaggactg cttgggcaat tgggggcgtt
     3061 cgaggctcag aaacagcatc gtgcctccgg ctaccgctgc cggcatgaag aagaggttga
     3121 ccaggggcac gaagaggaga atccagacgc ccgtgccgaa gcccgccacg gctggaagct
     3181 cggcccgggc gaatgagagc cggtcgcgaa ccgaccagtc gcggcgcgcg gcgggccagt
     3241 cgatgtagtc gaccccgaga tagaccgccg tgagcacaaa gccgacgaga gagacgagtt
     3301 gaccaatacc gggcaaaaag atcgaagcga ggaacatcgg tccgacgaca ccgaggtaga
     3361 ggatcacctt caagatctct aggcggaccg tccgtgcgac gtcagcaagc atatgactca
     3421 ccgaaaacgg gggcgcggcc cgacccgtga gcatgtgctc gaccgcttcg ctcaaggcgt
     3481 cgttgaatgg agctgccact acgctcgaca gcaagaggac cagcacgaga ccaagcacgg
     3541 cgatcaagag cccggccacg agctcgatca gccaacgcag cgcgctcagc aagcctccaa
     3601 cccagccgct ggcctcattc cagctttccg ggaagagcga ccaaaccatg ctcccgacgt
     3661 cgtcgtagaa gcttccagcg ccgtaaaaga cggcagcgag ggccaagcca gtgatcagta
     3721 tcggaaacac ccagtagcgc gcgagcttgg gatgctggaa gtagacgaag cgcatgcccc
     3781 gaaacgcata cgacaacccg cgcgcaaaac cgactggaga cctcgcaacg gcccccgcgc
     3841 cccgagctac gcctttcatg aagccaccgg ttcgaatcct agtccaggca aggtgaatga
     3901 gccatgacca ctcctgcgat gtgtcggcgt cggcagagct tgcagtcgcg ctggagagcc
     3961 gctatggatt tccagcttga ctcatgggat cgacgcggtg accgcagcca tactaggcgc
     4021 tctgcaaggg ctgaccgagt ttctcccggt gtcgagcagt ggtcacatcg ccattggcag
     4081 ccagttcttc ggcatccaag agaactcctt agcgctggtc attctcctgc atctaggaac
     4141 tttgctagcg acgctggtcc tctttcgaca agacgtctct gcgcttgccg ccgaggcgct
     4201 catggccgtt cgcgagccgc gccgactcgg gtcgacgccc gaggggcgca tgctggtgtc
     4261 gctcgcgctc gcgaccctag tcaccgggat gctaggcttt ctgctgcgaa agtatgccga
     4321 agcgttcgcc gaagattcga gtctggttgg ctatggtttt ctcatttcgg ctgctttcct
     4381 gatcgcgagc agcgtcgcgg tcggtacccg ctccgacgta tcttggcgac aggcaatcgt
     4441 gatcggcatg gctcagggcc tcgccgtgct gcccggtgtc agccggagcg gcctgacgat
     4501 caccgtcgga atgctcctcg ggattcaggg taacgctgcg tttcggttct cgtttctcgt
     4561 ttcgcttccc gctatcgtcg ccgcagcggt ctacgaggca agcggagcgg acgggctcgg
     4621 gtcgcttggt gcccaggcct ggatgggtgg cctgacggcc ttcgtgacgg gataccttgc
     4681 gcttgttatt ctccggcaag tcatactcgt cggacgcatt tggatgttct ccatctatct
     4741 gttgcctctc ggcctgttcc tcgtcactcg gtgaagtggc agccagctca tgaacaagaa
     4801 cgaaaaggca tatgcagtga tcatggcggg cggctcgggc acacgcttct ggccgctgtc
     4861 ccggagcaca cggcccaagc aattcatcgc gctcgggcct gacgatcgct ccctcttgcg
     4921 ggcgaccgcc gaacgcgtct gggacttgct tccgccagag cggaccttcg tcgtgacttc
     4981 cgagctcctc cgcgatcagg tcgcgaacga gctcccagag ctcgacccaa gccggatcct
     5041 cgcagagccc gttggacgca acaccgcccc ctgcatcggc tgggcggcca cccatgtgaa
     5101 gcggctagat gcagacgcca tcatggccgt gctccctgcc gaccattata tccgcgacac
     5161 cgaagcgtac ctcgagactc tgcgccgtgg tctcgaggct gccacgcatg gggactacgt
     5221 caccatcgga attcgtccgc accgcgcaga aaccggctat ggctacatcg aggtcggcgc
     5281 cgagatcgac cccggcgttt ttcgcgcacg ccgattcgtc gagaagccca accggcagcg
     5341 tgcggagcaa ttcgtcgcaa gcggcgggtt cctgtggaac agcgggatgt tcttcttcct
     5401 cgcttcacga attctcgatg cgatcgacca acacttgcct ggcctcggtg aagagctgaa
     5461 gcgctacgac gaggcagcaa aggccggcaa cgaagaccgt gcggtgcaag caacctatg
//
LOCUS       DASRZF010000092         5224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1098982, whole genome shotgun sequence.
ACCESSION   DASRZF010000092 DASRZF010000000
VERSION     DASRZF010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 5224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5224
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1098982"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VFG22_04105"
     CDS             <1..295
                     /locus_tag="VFG22_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415453.1"
                     /translation="PKRVTAPTSEAPPPAATEEILEDVIEDVIEEEHSSAEQAAPVEE
                     ALPDPQPRAAPAKAAPKPTTPRTPSVQRPVEEEKGPPASRPGYQKPPDLVTKW"
     gene            289..1143
                     /locus_tag="VFG22_04110"
     CDS             289..1143
                     /locus_tag="VFG22_04110"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET6415454.1"
                     /translation="MVRTVYIALSLWVASIAMLAPAPRSALAQDEQIQVHARELFAEG
                     LAFAEADRWAWALSAFRRSRDLVPRASTSYNIANALYRLDRPVEALSELDEYDRMPEV
                     QSDAAAAERGATLRALLRDIVAEVNLTVTPGNAIVLIDDRPSLLEGSHRTILLNPGAH
                     ALRVESEGYEPYRREIRVNRGGHKALLVELRARPSSKAAEPTVDIAPSAITISTADTG
                     LPGPDPVEDDRKPFVKRPGFWVMIGVIAAAGIGAGVAVALLRKDDAPACGTTGDCATT
                     QGLTVNSF"
     gene            1174..2385
                     /locus_tag="VFG22_04115"
     CDS             1174..2385
                     /locus_tag="VFG22_04115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6415455.1"
                     /translation="MRVAYLMNTYPLISTTFIRREIHALEELGVQVERFAVRAWEEQL
                     VDPRDVEERGKTEYLLTGNVRGLMTSFLKELVVNPRGLLRGLRTTYRLWRTAGADLVK
                     HAAYLLEAIRLRQRMAASRLQHVHVHFGTNATTVAMLSRAMGGGTYSFTAHGPDEFVE
                     PFRSSYDLKVRDAAFVAAISNFCRVQLARIAGMECWDKIEIVPCALDLRDFANKPDGT
                     HLNQQLVCIGRLCRQKAQTLFPDAIEPLSDAFPDLKIVLVGDGETRRELEDKIASKGL
                     GKHFEIVGWCSNEEVRKRLMGARAMLLPSFAEGLPVSIMEAFALRKPVISTYIAGIPE
                     LVDSSCGWVVPAGDVPALTAAIRNCITRGEMELNTMGDVGRSRIEARHDIRLSAALLR
                     EAFERYSGERS"
     gene            complement(2399..3421)
                     /locus_tag="VFG22_04120"
     CDS             complement(2399..3421)
                     /locus_tag="VFG22_04120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6415456.1"
                     /translation="MTRRPVIVNVSESADPAWNWIGGRFAEDPFRWISFSGLPRSAFE
                     DRIQSPKISRYRACWEAARAAKRERAALIVSHTPLVSAWTNLFCRALGVKAPHLAFSF
                     TFSTLPTGVRRRFFVNGIEGIDRFVCFSSVEQARYPKYFGIPEGRIDSLRWTAAEPGF
                     DSSARPSEPTPYLCAVGGEGRDYATLVEAMRRLPDHRLAIVARPANVVGLDLPSNVSL
                     RTNIPLADVWNVIAHAKLMVLPLRDTEVPCGHGTLIAAMQLETPSIVTESAAMTDYVS
                     DETTGLITPPGDPDAMARTIERLWDDEALARRLALNARAFATEECSERRTVTYFMDYA
                     RSLGLL"
     gene            complement(3492..4490)
                     /locus_tag="VFG22_04125"
     CDS             complement(3492..4490)
                     /locus_tag="VFG22_04125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6415457.1"
                     /translation="MTAASRFSVGAVAIGKNEGERLRICLESLAKQVSHLVYVDSGSS
                     DGSVELARSVEAVVVELDPRIPFTAARARNAGVERLTEIAPGLKYAQLIDGDCELVEG
                     WIEDAVDFLEANGEYAAAAGRLRERFPDASVFNYLCDVEWNTPVGEAKSCGGIAMMRI
                     RAFESVGGFMPDLIAGEEPELCVRLRQEGWKIRRLGEEMALHDAAMKRLSQWWKRGMR
                     AGHAYAEGSHLHGAPPERMGVRESRRIVAWGLILPTLAVAAAPLTGCSSLLLLLAYPL
                     NVIRIARRLRSDGETRPWTLAFFLMLGKFPEAIGWIRFQWGKLVGNRSAIIEHKDA"
     gene            complement(4487..>5224)
                     /locus_tag="VFG22_04130"
     CDS             complement(4487..>5224)
                     /locus_tag="VFG22_04130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6415458.1"
                     /translation="AGNNAGIRPYLDTEEPPAYVMLLNPDACVHRGAISTLLAFMESH
                     RDVGIAGTRVEGADGKQAHSAFRFPSAASELVEGFKLGPLTRLLSDRQLLYELGDEIM
                     QVDWVTGAAMMIRREVLTDVGLFDDRYFLYFEEVDFCFRARRAGWSTYYVPDSVVTHL
                     QAQATGITDNRKDKRRYPGYWFDSRRRFFVKNRGKAQAVLADLVFLVGYTTFRARAVI
                     QRKENEEPPHFWGDFVRNSTFVKGFEV"
BASE COUNT         1034 a         1639 c         1613 g          938 t
ORIGIN      
        1 gcccaagcgg gtgaccgcgc cgacgagcga agcgcctccg ccagcggcca ccgaggagat
       61 cctcgaggac gtcatcgagg acgtcatcga ggaggagcat tcctctgcag agcaggccgc
      121 gccggttgag gaagcgctcc cagatccgca gccacgggcc gcgccggcca aagcagcccc
      181 aaagccgacc actccgagga caccgagtgt gcaacgcccg gtcgaagaag aaaagggccc
      241 tcccgcgtca cgcccgggct accagaaacc gccagatctc gtgacgaaat ggtgagaacg
      301 gtctacatag ctctgtccct ctgggtcgcg tcgatcgcaa tgctcgcgcc ggcgccgaga
      361 agcgcactcg cgcaggacga acagattcag gtgcatgctc gcgagttgtt tgccgagggg
      421 ctggcgtttg ccgaagccga ccgctgggca tgggcgctgt ccgcatttcg gcgctcccgc
      481 gacttggttc cacgcgccag cacctcgtac aacatcgcca acgccctcta tcgactggac
      541 cggcctgtag aggcgctctc ggagctcgac gagtacgacc gcatgcccga ggttcaaagc
      601 gacgcggccg cggccgaacg gggtgcaaca ttgcgcgcgc tgctccgcga catcgtcgcg
      661 gaggtgaatt tgacggtgac gcccggcaac gcgatcgtgt tgatcgatga ccggccttcg
      721 ttgcttgagg gcagccaccg aaccatattg ctcaaccccg gcgcgcatgc gctaagggtc
      781 gaatccgagg gatacgagcc atatcgccga gagattcgag tgaaccgggg ggggcacaag
      841 gctctcttgg tcgagctgcg ggcgcgccct tcgagcaagg cagccgagcc cacagtggat
      901 atcgccccgt ctgccatcac gatctctacg gccgatacgg gtttgccggg gcctgaccct
      961 gtcgaagacg atcgcaagcc cttcgtcaaa cgccctggtt tctgggtgat gatcggtgtg
     1021 atcgcggctg ccggtattgg agcaggggtc gccgtcgcgc tattgcgcaa ggacgatgca
     1081 cccgcatgtg ggacgacggg cgattgcgcg acgacgcaag gtctgaccgt aaactccttt
     1141 taattaaccg tgaccgaaca ccggaggcaa gctttgcgag tcgcgtactt gatgaacacc
     1201 tacccgctca tcagtacgac gttcatccgt cgggagatcc atgcgctcga ggaactgggc
     1261 gttcaggtcg agcgcttcgc agttcgagct tgggaggagc aattggttga tcctcgcgat
     1321 gtcgaagaac gaggcaagac cgaatacttg ctcacgggca acgtccgcgg actgatgact
     1381 tcgtttctga aggagctcgt agtcaacccg aggggcttgt tgcgagggct gagaacaaca
     1441 taccggctgt ggcgcaccgc cggagcagac ctcgtcaagc acgccgctta cctgctcgag
     1501 gccatccgtc ttcggcagcg catggccgct tcgaggctgc agcacgtcca cgttcacttt
     1561 ggaaccaatg cgaccaccgt ggcgatgctg tctcgtgcga tgggaggagg cacgtatagc
     1621 ttcacagcac acgggccaga cgagttcgta gagccttttc gctcgagcta cgacctcaag
     1681 gttcgtgacg cggcgttcgt tgcagcgatt tccaactttt gccgcgtgca gctcgcgcgc
     1741 atcgctggga tggagtgctg ggacaagatc gaaatcgttc cgtgcgccct cgacctgcgt
     1801 gatttcgcaa acaagcctga tggcactcac ctaaaccagc agctggtttg catcggccgc
     1861 ctgtgccgac agaaagccca gacgcttttc ccagacgcca ttgaaccact atccgatgcg
     1921 tttccagact tgaagatcgt tctcgtcggt gatggcgaga cacgccggga gctcgaagac
     1981 aagattgcga gcaaaggcct cggaaagcac ttcgagatcg tcggatggtg ctccaatgaa
     2041 gaggtgcgta agcgcctcat gggcgcccgg gccatgcttc tgccgagctt tgccgaaggg
     2101 ttaccggtat cgatcatgga ggcctttgcg ctccgcaagc ccgtgatctc cacctatatt
     2161 gcggggattc ccgagctcgt cgattcgtcc tgcggttggg tcgttcctgc gggagacgtt
     2221 ccagcgctga ccgctgccat ccggaattgc atcacgcgcg gcgagatgga actgaacacc
     2281 atgggtgacg tcggccggtc tcggatcgag gcccgtcacg acatccggct ttccgcggcg
     2341 ctcctgcgcg aagcgttcga acgctacagc ggcgagcgga gctagagttc caacgaacct
     2401 agagcaggcc caggctccgc gcatagtcca tgaaataggt cacggtccgc cgttcgctgc
     2461 attcctcggt cgcaaaggcc cgagcattca gcgccaggcg cctggcaagc gcctcgtcat
     2521 cccagaggcg ctcgatcgtt cgcgccatcg cgtcggggtc accaggagga gtgatcaggc
     2581 ctgttgtctc atcgctcacg tagtcggtca tcgcggcaga ttcggtcacg atggaagggg
     2641 tctccagctg catcgctgcg atcaacgtgc cgtgaccgca cgggacctcc gtgtcgcgga
     2701 gcggcaggac catgagcttc gcatgcgcga tcacgttcca cacgtccgcc agggggatgt
     2761 tggttcgtag gctcacgttg gacggaagat cgagccctac gacgttcgcc ggccgggcca
     2821 cgatcgccag acgatggtcc ggaaggcgcc gcatggcctc gacgagagtc gcgtaatcgc
     2881 ggccttctcc accaaccgcg cacaggtacg gggtcggctc gctgggtctc gcggatgaat
     2941 cgaacccagg ctccgccgca gtccaacgaa ggctgtcgat tctcccctcg ggaatgccga
     3001 agtacttcgg gtagcgggcc tgctcgaccg aagagaaaca gacgaatcga tcgatgccct
     3061 cgatcccgtt gacgaagaag cgtcggcgga cgcccgtcgg cagtgttgaa aaggtaaacg
     3121 aaaaagccaa gtgcggagcc ttcaccccca gggctcggca aaacaggttc gtccaggcgg
     3181 ataccagtgg agtgtgggac acgatgagcg ccgcccgttc gcgcttggcc gcccgagccg
     3241 cctcccagca ggcacggtag cggctgatct tcgggctctg gatcctgtct tcgaacgcgg
     3301 agcggggaag gccggagaag ctgatccagc gaaaagggtc ctctgcgaag cgtcccccaa
     3361 tccagttcca agcaggatct gcggactcgg atacattcac gatgacaggt cttctcgtca
     3421 cgcccgaaat ctagcatcgc gggaagcgaa atgccgagcg ccgctgagga aaccggctga
     3481 caagggtagc ttcaggcgtc cttgtgttcg atgatggcgg agcgattccc aacgagctta
     3541 ccccactgaa agcggatcca gccgatcgct tcgggaaatt tgccgagcat caggaagaaa
     3601 gcgagcgtcc aaggtcgcgt ttcaccgtcg cttcgcaggc ggcgggcgat acgaatcacg
     3661 ttgagtgggt aagcgagcag caacagtagc gaagaacagc cggtgagggg tgcggcggcg
     3721 acggcgagcg tcgggaggat gagcccccaa gcgacgatgc gcctcgactc gcgaaccccc
     3781 attctttcag gcggggctcc gtgcaggtgt gacccttcgg cataggcatg gccggctcgc
     3841 atcccacgct tccaccattg agacaggcgc ttcatggcgg cgtcgtggag cgccatctcc
     3901 tcaccgagac ggcgtatctt ccaaccctct tgccggagcc gcacgcaaag ctcgggctcc
     3961 tcgcccgcga tcaggtcggg catgaagcca ccgacggact cgaacgcccg gatgcgcatc
     4021 atggcgattc caccgcagct cttggcctcg ccaactgggg tattccactc gacgtcgcaa
     4081 aggtagttga agacgctcgc atcggggaaa cgctccctca gccggccggc ggcggcagcg
     4141 tactctccgt tcgcttccag aaaatcgacc gcatcttcta tccatccctc gacgagctcg
     4201 cagtccccgt cgatgagctg ggcatacttc aatcctggcg cgatctcggt tagacgctcg
     4261 acaccggcgt tccgcgcgcg cgcagccgta aacgggatac gcgggtcgag ctcgacgaca
     4321 acggcctcga ccgagcgcgc gagctccacg cttccgtcgg aggagcccga atcgacgtag
     4381 acgaggtgag acacctgctt cgcgagcgac tcgaggcaga tcctcaaccg ctctccctcg
     4441 ttcttgccga tcgcgacggc gccaaccgaa aagcggctcg ccgcggtcat acttcgaacc
     4501 ccttcacgaa cgtgctgttc cgtacgaagt ctccccagaa atgaggcggt tcctcgtttt
     4561 ccttgcgctg gatgacggcc cgagcgcgga aagtcgtata gccgacgaga aacactaggt
     4621 ccgcgagtac tgcttgcgcc ttacctcggt tcttcacgaa aaagcgccgt cgtgaatcga
     4681 accagtagcc tgggtaccgg cgcttgtctt tgcgattgtc ggtaatccca gtcgcctgcg
     4741 cttgcaaatg cgtgacaacg ctgtccggaa cgtaatacgt cgaccaaccc gctcgacggg
     4801 cccgaaagca gaaatccacc tcttcgaagt ataggaaata ccgatcgtcg aagagtccca
     4861 cgtcggtcag gacctctctg cgaatcatca tggccgcccc ggtcacccaa tcgacctgca
     4921 tgatctcgtc gcccagctcg tacaggagtt gtcgatcgct caggagccgg gtgagcgggc
     4981 ccagcttgaa tccctcgacc aactcgctcg cagcgcttgg aaaacgaaat gccgagtggg
     5041 cttgctttcc gtcggcgcct tcgacgcgcg taccggcaat accgacatcc ctgtgacttt
     5101 ccatgaatgc aagcaacgtg ctgattgcac cgcgatgcac gcaagcgtcg ggattgagga
     5161 gcatcacgta ggccggaggc tcttccgtat cgaggtaggg tcggattccg gcgttgttac
     5221 cggc
//
LOCUS       DASRZF010000093        27346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3163334, whole genome shotgun sequence.
ACCESSION   DASRZF010000093 DASRZF010000000
VERSION     DASRZF010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 27346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27346
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3163334"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VFG22_04135"
     CDS             complement(<1..329)
                     /locus_tag="VFG22_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415459.1"
                     /translation="MSSLMGVSPLLILVAAGLAIMLVDAFTKENAGIAIVTTVSLFAA
                     AAVAGAMLVSADIAPAPALVTRYLAVDRMGLFFDVVIAVGAGLSALLAGGYLREHGFE
                     RGEFYVLL"
     gene            complement(326..1954)
                     /locus_tag="VFG22_04140"
     CDS             complement(326..1954)
                     /locus_tag="VFG22_04140"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HET6415460.1"
                     /translation="MTTQYLTWLLALPAISAIIVLLMPRSWESGIKRFSIGAMLLELI
                     ISLRLVLADYSGAGYHFVEQVTWVESFGISYKVGVDGISLWLVILTTLLSPIALYASW
                     GSITTRIKEYAFSFLLLELGMLGAFVALDLFLFYVFWELMLVPMYLIIGIWGGKDRVY
                     AAVKFFLYTMVGSLLMLVAILYLVASYKELAGHYSFDLEALSSLVLPAMPQYLLFSAF
                     ALAFAIKVPMFPFHTWLPDAHVQAPTGGSVILAAVLLKLGIYGFIRFAMPLFPVASQA
                     IGPVLAGLGVVGIIYGAYAAWVQRDLKKLVAYSSVSHMGFILLGLFAMNRHGISGAIL
                     QMINHGISTGALFLLVGVIYDRRHSRMLDDFGGLAKVMPMYTLVFVIITMSSIGLPGT
                     NGFVGEFMILAGSFFSRTPIGYGSSWFIMTLLAATGVIFAAVYMLHAVLRIFWGKISH
                     SENESLPDLNRREIVSLLPLIVMVFWIGLYPKFFLDKMNPTVDSFVTDFTVRYYEGRR
                     TSEAHLVDFDQLRQRIEAAGGLAMVEPNDEEVGR"
     gene            complement(1951..4554)
                     /gene="nuoL"
                     /locus_tag="VFG22_04145"
     CDS             complement(1951..4554)
                     /gene="nuoL"
                     /locus_tag="VFG22_04145"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HET6415461.1"
                     /translation="MHEEAHQQLLLLLIILFPLAGAIINGLFGRYMPKGIVTSVGVGS
                     VAVSFALAVTSFIQLYGLPHEEAHAVLSYQFYEWFSLTLPGGNVVPINVRFLMDSLSG
                     VMTLVVTGVGGLIHLYSIGYMGEDEGYPRFMSYLNLFMASMLILVLGSSLPVMFVGWE
                     GVGLCSYLLIGFWYENGDYAAAGRKAFVVNRIGDFGVLIGMFILVGVAGSFEFAEINQ
                     AAMHGEFRPDFPIWIFGVAPSLATVACVFLFLGCTGKSAQIPLFVWLPDAMAGPTPVS
                     ALIHAATMVTAGVYLCCRLSPLFITSDVAMSIIAVTGTLTALLAASIAVVQREMKKIL
                     AYSTVSQLGFMFAAVGVGFFSAGFFHVFTHAFFKACLFLGAGSVMHAVHAHGDADIFK
                     LGGLKKVLPITRWTFLASCLAIAGFPLTSGFFSKDEILLGAAAQIYRPDDALMTAVGW
                     FTLIGLTLAAVMTAFYMFRLYFLTFTGEYRSADQSGDHPYDAHPHESPPSMTIPLVVL
                     GIGALLVGFLGLPHVMPITGTHLYSWWGHWMESSVAGQPVPEEMHIVNLASGLAFGAM
                     AIGISAAWVLYRNKSHDALAERMPKRLFQLAFDKWRVDELYAMTIVNPIKKIATVAGE
                     ADMTFVDALMTRVPSFNVRETGRLFARMQNGVVQMYGTAMLVGVVAVMTWFWTPHSRI
                     ETVFEGTRVALSTPGGLGYEYRWDANSDGDFETAWDRSPETSFEYAEDDIRGIAVFLV
                     NVHAGIERRIPITDDWSPLPLESVVPIEQIMPQDEGFEIRIDGTDLLFRKPHAKTKLS
                     GSKEIRLPLGKDGRLGPARVFARPLVEATVEVRNAFGNLRRDTKEIALPFGVEAPSHA
                     SLMLPADEVVR"
     gene            complement(4559..4861)
                     /gene="nuoK"
                     /locus_tag="VFG22_04150"
     CDS             complement(4559..4861)
                     /gene="nuoK"
                     /locus_tag="VFG22_04150"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HET6415462.1"
                     /translation="MGIGTYLALATVLFGIGAVGFLTRRNMLIQLMSIELMLNSVNVV
                     LLAFNRVYPANHDGQVFVFFVIAVAAAEAAVGLAILIAYYRIKKTVDTDDATLLKY"
     gene            complement(4868..5371)
                     /locus_tag="VFG22_04155"
     CDS             complement(4868..5371)
                     /locus_tag="VFG22_04155"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HET6415463.1"
                     /translation="MIGPGTILFVSTAILAMAGALITISSRRPLRAAIGLLIHIISLA
                     GMFLTLNAELLAALQLLVYAGAVVVLFVFVIMLIGPAAEVDAMQGRVSSRALSIALTI
                     GVVLAVSTSVAYFDVPWGVVSEDFGTVQGLGTAVYKQALVPFELISITLTVAIIGAIA
                     IARSRTE"
     gene            complement(5368..6744)
                     /gene="nuoF"
                     /locus_tag="VFG22_04160"
     CDS             complement(5368..6744)
                     /gene="nuoF"
                     /locus_tag="VFG22_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HET6415464.1"
                     /translation="MSQPIKPLLTARYGIAESWKLANAEAAGAYRVARQALTTRKPAE
                     LRDEVKKANIRGRGGAGFPAGVKWGFLNKQEGEEIYLVINADESEPGTFKDRSIMDLD
                     PHRLVEGAIISCFAIDAHVAYIYVRGELAFSIGRLEQAIADARKAGYLGQRPFGIDHS
                     IEVYVHSGAGAYICGEETALLNSLEGRRGEPRLKPPFPAVKGAFGMPTIVNNVETIAS
                     VPDIVEMGGDAWAALGRLPGDGGLRIYGVSGHVKNPGLFEAPVGLTMRELIDDYAGGM
                     LGNRPLKAVIPGGSSTPVLDPRRKVEAPNADHPLHKWHGKSEIDVPMGVDTYRALGTM
                     LGTCCAIVMDDSVNMVEACRNLMRFYKHESCGQCTPCREGTGWLVDVLDNLCAGRGKP
                     EDVDLLVNISNNMMGNTICAFADGTAMPMLGMVQKFREEFMEAALRGVPDDVRHDDSV
                     RSTVMGGA"
     gene            complement(6741..7400)
                     /locus_tag="VFG22_04165"
     CDS             complement(6741..7400)
                     /locus_tag="VFG22_04165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HET6415465.1"
                     /translation="MVFSLTREREQQVDEILTRYPERRAALLPVLWLCQRQNGWISPE
                     VVEYVSERLELSTAIVKGVVTFYTMFFDEPVGENVVWVCRTLSCDLRGGKAIQDHLEQ
                     KLGCTAGHTSADGKFTLLKAECLAACGQAPMVQINDFYYENLDVERLDRIIDAHANEG
                     GEAAAAEFSTFARFGSPRQRGGSPTSAPPPPSKEAVRLASQPPPAAATPDLGDTRDED
                     S"
     gene            7476..7994
                     /locus_tag="VFG22_04170"
     CDS             7476..7994
                     /locus_tag="VFG22_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415466.1"
                     /translation="MSENAATPPSKSVGLWVGLGCLGVLVFSCCLLTFWAQSYGLRFV
                     LGQDDGTKVWFSRMILVGALEGTRKTCSDGVVSEDTLPWFHPELPAESRNLICSLDET
                     TLQALSTAERASATPLLRTDRSELAARFGMDAAQCFQHSTDGLTAVGCFDTESGPGSI
                     PYQIIDLRVTQP"
     gene            complement(7981..8586)
                     /locus_tag="VFG22_04175"
     CDS             complement(7981..8586)
                     /locus_tag="VFG22_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415467.1"
                     /translation="MRRGFGWLPLGVVLSGFLMGCTGRAEAVRAAQPYIEDFGALRSR
                     ALSLLELRSDVERRRLLAKAMAGDEGSLPENLKPMLERMQAEGAEVTEQQLQHGETLF
                     WRMLDYTFSENPNILQAEILFLEKDGSVDSLRHPREREIPVGVKWHGLREHRTFAALT
                     DCVTDEGSEPCVLLQLRPRDYPGSAGLTVAYRRTPIDVTAE"
     gene            8637..9410
                     /locus_tag="VFG22_04180"
     CDS             8637..9410
                     /locus_tag="VFG22_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6415468.1"
                     /translation="MSLIEFRGVQKAFGSKVVYRDLNLEVRGREVLTIIGGSGQGKSV
                     MLKMLIGLLSVDGGAIYFDDQQISGMSEAQYAAVRRRVAMLFQASALFDSLNTKENVA
                     YGLREQLDMSEKEISERVRESLTYVGLPGTEETWPADLSAGMKKRVALARALAIRPEV
                     LLYDEPTTGLDPINVTRIADLIEYLNETLDVTSVVVTHDMDTALAISDCIAMIHEGFV
                     IFSGTPDELRASDNPQVRDFIEGNAPVDEDTQTLLRSAG"
     gene            9431..10558
                     /locus_tag="VFG22_04185"
     CDS             9431..10558
                     /locus_tag="VFG22_04185"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HET6415469.1"
                     /translation="MKTTAGTRLRVGIFVLVALVIGASVAFAIGAQENIFSSKTEYYA
                     IFHDVGGLQKGNAVRVAGVNVGSVTEVVIGDDGRIEVYFRIIDSATHLIRGESGALLD
                     SDSPPSSFAAIGSKGLLGDRLIEISVGSHEYPEWDPDEPIPVSVGGGIMALAERTLVE
                     AEGTAHNLRLATDPFSDQEFSNDLKETARNLAKATGLIASGDGTIGRLMNDAELGRDV
                     KGAVKELRAAGKQVAALSKNLNKITEDIQTNEGTAHALLYGEEGADAIRNIRDTFAQL
                     NTILTDVREGDGAVNQLIYGELGESFMDNLQSASDDIAFLAKEAREGKGTIGALLTDP
                     SIYEDIKRLVGDLERNDILRALVRYSIRRDDPREPVEVLEE"
     gene            10563..11405
                     /locus_tag="VFG22_04190"
     CDS             10563..11405
                     /locus_tag="VFG22_04190"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HET6415470.1"
                     /translation="MQAYLDLLRKVLNDGVARDDRTGTGTRSLFGYQMRVDLRDGFPL
                     LTTKKVHLKSIIHELLWFVRGQTQVSHLQEVGVSIWDEWATAEQTARFGRKEGDLGPV
                     YGHQWRNFGATERADGTYERDGFDQLSYVLDQIRDNPDSRRLIVSGWNPSEAERVALP
                     PCHTLFQFYVSNGRLSCQLYQRSADVFLGVPFNIASYSLLTMMIASVAGLEPGDFVHT
                     FGDVHLYSNHIEQAREQLSRQPRPLPKMWIDPSVQSLFDFRFEHFRLEDYDPHPRIKA
                     PVAV"
     gene            11402..11911
                     /locus_tag="VFG22_04195"
     CDS             11402..11911
                     /locus_tag="VFG22_04195"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HET6415471.1"
                     /translation="MSDTLAGPAPLALIVAVARKGVIGKDGGLPWHVSEDLKHFKATT
                     SGHAIIMGRKTYDSIGRPLPKRRNIVVTRRIGAEIPGCEVAGSLDEALALARTSDPCP
                     FIIGGASLYEEALPIATELYMTTIDENVEGDTYFPEDLPDFEEVEARAGQTSGIVFRV
                     LKRKSTRTR"
     gene            complement(11934..13247)
                     /locus_tag="VFG22_04200"
     CDS             complement(11934..13247)
                     /locus_tag="VFG22_04200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HET6415472.1"
                     /translation="MFRHKSSRNVLRLTVVGALALLLVAATASNAPARRSPFEPLSIF
                     ARALAYVELSYIEPVDQESLVYGAIRGLADSLDPHSVFLDPVEYDILKSDAEGRFAGV
                     GVEVSTRDGWLTILSVFEGGPAAKAGLRPGDRFLTIEGEDARDLRLYDAVQKIRGEPG
                     TSVKVSIRREGFAKALERTLTRAFIDLDPIDMELLGDGIVYLRIKAFQDGTARMLSDA
                     LDEAVMKLRKRDGVRGLLIDLRDNGGGLLHEAVWVSDQFLASGVIVSTRGRGGEMVGQ
                     YSARRAGTRPKWPIVVLINENTASAAEIVAGALHDHHRAELVGVRTFGKGSVQNVFEL
                     PDGSALKLTTYRYYTPSGNSIQAEGISPDLLAEQPRPEGDPEPFREEELDGHLAAQSK
                     GLGGSANAPAPNQAPLASGSVPKMLAEDSQGQRAYTVLKSQVGGR"
     gene            13361..13561
                     /locus_tag="VFG22_04205"
     CDS             13361..13561
                     /locus_tag="VFG22_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04563 family protein"
                     /protein_id="HET6415473.1"
                     /translation="MSTTDKRKQSLYFPESMLHDIKHESARLDRSLSWIVQRCVKIAL
                     PEIRKLPSVNDIPDSPDDDGKK"
     gene            13573..14463
                     /locus_tag="VFG22_04210"
     CDS             13573..14463
                     /locus_tag="VFG22_04210"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6415474.1"
                     /translation="MRYERDGFVEAEDGTRLFYEELGGGNPPIILTDGIGCDGFVWRY
                     LMPNLARHHRVVHWHFRGHGRSGPPRNPEQLSIEDLARDLRTICDHLEVERPVIAAHS
                     MGVQVALEYYRQNPDDVAALILMCGTFGRITSTFHGTDLLDQLLPSIALGARMFPNVA
                     RAIWGRVPAAMAFRVACAGRELDAERIQEEDFQRYWEHSSLMDPEVFLPMLQLAGQHD
                     TRGFLPEVAVPTLVVAAEHDTFTPMALAEEMAGAIPDAELEVIEEASHAAPVEQPDRI
                     TERIEDFLSSRLGLQRARPQ"
     gene            complement(14457..15512)
                     /locus_tag="VFG22_04215"
     CDS             complement(14457..15512)
                     /locus_tag="VFG22_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET6415475.1"
                     /translation="MIVCSRCSKDNQDHYKFCLGCGAELPRHSSQPKSFSAATPPGGT
                     PSRGRTTNAPPARAAQAEALQMDPEPLTAEDAPLPELAPLGDETAPCSHCGAEVPENF
                     RFCHVCGHDLHAEASDGASADLPAERVSAPAVAETSLPATARAELILIQPDGSEGEAF
                     SLSARGSLIGRSTGGIFANDAYLSPEHAEFRFESDQLVVRDMESLNGVYIRIEPEVPI
                     ELFDGSIFRVGQEILRFEAPFAPDPAPDGTQHMGSPHRGYLGRISLLIGRQTTGNSYP
                     IPPEGIHLGRERGDIVFPEDGYVSGLHCRVHSEVGHVFLTDLGSSNGTFVRVLNESYV
                     PPSTFVLMGQQLFRADY"
     gene            complement(15509..16297)
                     /locus_tag="VFG22_04220"
     CDS             complement(15509..16297)
                     /locus_tag="VFG22_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET6415476.1"
                     /translation="MEQARNYVCTECASAVPSGHKFCGACGANVPAEAQTLATRFFGA
                     LQMPGKARLIVVRGDEAMGEGLSYLLQATEHVAGREADQIPFPSDNWLSPSHANFLYR
                     GEKLVVRDEGSLNGVYIRIRGTVPIQIGDHFMCGQQLFRVDATPKDTSGPEADQTYFY
                     ASPRRPSAFRITQVLEGGMDGIVCCAREQSVQIGREDCDINFPDDVYMSPRHARVEMS
                     GESLALVDENSQNGTYVRIRGERELSHGDYVFLGRNLLRVEVTA"
     gene            16453..17121
                     /locus_tag="VFG22_04225"
     CDS             16453..17121
                     /locus_tag="VFG22_04225"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HET6415477.1"
                     /translation="MELLAGALLERPPGPKYTSELRFAELSPQAPLPRPDTLAKWREK
                     LPKGFELALRAPDVCWQSPTGPLRPSEELDAGLAWLSEAADALQASMLVVATNAAVTT
                     GARDRERLREYFARIPRNDARLLVWQASGLWEHEGVQSMARKLSVLGAFNAADDPAPD
                     DAEIVYASLRAEGLRRSFSHAHLLDVLDKIEGSGASRAFVTIESAQSFREARLLQALS
                     EGRE"
     gene            17118..18443
                     /locus_tag="VFG22_04230"
     CDS             17118..18443
                     /locus_tag="VFG22_04230"
                     /inference="COORDINATES: protein motif:HMM:NF017633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HET6415478.1"
                     /translation="MSAWNARIARVDQPEPGLLSLSFRTEGRNEALVLVTLPGSLDLG
                     VVDERPRGASAGPAVTQLRRHVEGSRIESVDVSGRAIRLSLSRAGTNRFLIAAPSKPY
                     GAWWLCEEDGALVLRSPGAPASMPSEQEHLRPKGLDELRVRGLAVLQAHRSTRKQQLE
                     RLLAAQIKRLSKKRDAILGDLDRAAKAGELHEKASLILAHASEIPERAAYFDTQTWDD
                     EPRAIRIELDPRRTPTELAQELFKKSKRFKRGSDVAPQRLSAVVAELEKLIEVRDRLE
                     DETAEELATKLEALGVPITAPKEEARKRRQAGERLPYREFISVDGSPLLVGRSAADND
                     RLTLRHARPYDLWLHARGVTGAHVLVRLAKGTQCPPETLVDAATLAGHFSDLRGEAIV
                     DVLYTPRRFVHKRKGSSVGSVTLGSEKVIALRVEPARLERLLKNERRAT"
     gene            complement(18620..18961)
                     /locus_tag="VFG22_04235"
     CDS             complement(18620..18961)
                     /locus_tag="VFG22_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415479.1"
                     /translation="MATAKKRTTKKKRPAKKRAAKKAVKKAVKRRAAKKAVKKAVKRR
                     KAKKAVKKAVKRRAAKKAVKKRATKKRATKKRATKKRATKKRATKKRATKKKAAPKKR
                     AAKKRVARKKK"
     gene            19426..19935
                     /locus_tag="VFG22_04240"
     CDS             19426..19935
                     /locus_tag="VFG22_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HET6415480.1"
                     /translation="MQAGTYEFKVGDKAIYPKQGVTEVVSIEVRKIGTATMEFYVLRP
                     LDAKNNDRIRVAVKQAKKVGLRPPISETDIKEIFALLGEQPVVFDNQTWNRRYRGFMD
                     KIKTGSVFDVAEVMRDLYRLQVDKSLSFGEKKMLETARNLVVKEIAVARSKTEESTQA
                     EIEQIFVKN"
     gene            20080..21594
                     /locus_tag="VFG22_04245"
     CDS             20080..21594
                     /locus_tag="VFG22_04245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HET6415481.1"
                     /translation="MAKNTLLVNVDIGETRVGLIADGVLRELYVERRHHRSPVGNIYL
                     GRVTRVLPGMQAAFIDIGLDRAAFLHVEDLQAPPGSEEEPEGNDKRGPAKGRSNRVSR
                     KTPIRDLLNEGQQLMVQVSKGPIGTKGARVTSHVALPGRYVVFMPTVEHVGVSKRIGN
                     DRERRRLKEVIDSVKPPQGGVVVRTLAQGLTKKKLKADIGYLVKLWEQTQTAREAVKK
                     TPALVHEEPDLVIQSARDLFTEDISEIVVDDRDEYQRVKEFLTLFLPERANDVQLYED
                     DEPLFDEYGIEEEITRALSRKVHLPSGGHLVIDQAEALTAIDVNTGRFTGKGRDVEQT
                     ILQTNLEAVREIAYQLLFRNIGGLIVLDFIDMERQAHREKVFKALLNALKNDKSRTTA
                     IRISDLGLVEMTRKRTRQSLGRTLYEPCFFCDGTGLLQSKETVCHEIFRQMRRERDAL
                     PGYKIVISAHPAVCDMLEREEKASVAEAARRFQRRIELNPRNDYHLEQFDLTGD"
     gene            21601..21930
                     /locus_tag="VFG22_04250"
     CDS             21601..21930
                     /locus_tag="VFG22_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6415482.1"
                     /translation="MSNDDGQRIQKRVTINKEFESFDAFVHEYVTNVSRSGVFIRSKE
                     PLAVGTEVHLTFTVIMDDVETIEGAGEVVRVQDDPPGMGVAFTKLTKYSEDLLVRLLT
                     LHGAAGS"
     gene            21927..22265
                     /locus_tag="VFG22_04255"
     CDS             21927..22265
                     /locus_tag="VFG22_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HET6415483.1"
                     /translation="MTRLSEGKFGPSGKKLADLSDDEVLAERAKCRGERSTSESRPSW
                     KRVKQYLANLELNPGATWPEIERAYERLRERYNPEKHEADPARFRAAEELNASLASAY
                     QALRRYFRSE"
     gene            complement(22282..23235)
                     /locus_tag="VFG22_04260"
     CDS             complement(22282..23235)
                     /locus_tag="VFG22_04260"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HET6415484.1"
                     /translation="MGSQQGELVIEGLTLPLALRFISGKYQGGEFPLPDDGEIVIGRS
                     SELDMVLVEDMVSRRHAKISVENGNIFLEDLGSTNGSFVNGEKISRTKLAEGDRILIG
                     TSIIKVVASDGSASLQDAKAKMDAAASSARPASAASTMTGSIAEVALPDLLQLFSASK
                     KTGVLQITTESDVGSIYLDEGRVQFAVVNGDESVPPEKAFYRVLVWDKGTFDLLPKVE
                     RTFPTQIQGSIEGLLMEGMRQLDEMRRLGDDLPPLRSKMSVAFPLKKPLRDASPDELD
                     VIQLALNHNTLADMMNHSGRPDPDVAELVVALLEKGYLTST"
     gene            complement(23441..23998)
                     /gene="frr"
                     /locus_tag="VFG22_04265"
     CDS             complement(23441..23998)
                     /gene="frr"
                     /locus_tag="VFG22_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HET6415485.1"
                     /translation="MLDETLEELGASIDKAHESLRRDLAKIRAGRANPAILDGLRVDY
                     YGSQTPLKQLASMSVPEPRMIVLKPFDRSQMQVIEKAIREAQLGLNPSNDGEIIRIPM
                     PPLTEERRKDLVKVARKSGEECKVAIRKARHEAKDMVDSLQKAGDVGEDDADRARKEL
                     EDLVKTATAKVDEIVAKKETDILEV"
     gene            complement(24023..24751)
                     /gene="pyrH"
                     /locus_tag="VFG22_04270"
     CDS             complement(24023..24751)
                     /gene="pyrH"
                     /locus_tag="VFG22_04270"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HET6415486.1"
                     /translation="MTNGAKLSRVLLKLSGEVLSGGPAGKSIEPETLKAISEELAEVH
                     SSGCQIGIVIGGGNIFRGLKGSASGMDRTAADHMGMLATVINGIALQDGLEKAGVDTR
                     VMSALEIKAVCEPYIRRKAVRHLEKGRVVIFVAGTGNPYFSTDTAAALRAMEIRADAL
                     CKATKVKGIYDKDPVRHDDATMVRRMSYDRFLMERVGVMDATAVALCRDNNLPVRVFK
                     LTEPGNIKRVVMGEDVGSLVTAEG"
     gene            complement(24755..25429)
                     /gene="tsf"
                     /locus_tag="VFG22_04275"
     CDS             complement(24755..25429)
                     /gene="tsf"
                     /locus_tag="VFG22_04275"
                     /inference="COORDINATES: protein motif:HMM:NF013085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HET6415487.1"
                     /translation="MSEISASMVKSLRDRTGAGMMACRDALRETEGDEEKAIEIIQKK
                     GLSKVAKKAGAIAGDGLVHAYIHGAGRVGVLIEINCQTDFVARGDDFRQFVEETAMQV
                     AAENPLFVSEDDIPENELLGRMAIFRGQLEEEAKATGKEKPAAAVEKIVEGKLAKWKK
                     ESCLLDQVSILHDEKTIRQLLEALTAKLGEKISIRRFARYELGEGIEKKKSDLAAEVT
                     EALKGS"
     gene            complement(25429..26325)
                     /gene="rpsB"
                     /locus_tag="VFG22_04280"
     CDS             complement(25429..26325)
                     /gene="rpsB"
                     /locus_tag="VFG22_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HET6415488.1"
                     /translation="MAEPTLETQEAPELAALAGPDVASGDLPIGLRALIDAGVHFGHQ
                     TKRWNPRMRPYIYGARNGIHIIDLDQTAELFKRAYAFVTDSVGRGGHVLFVGTKRQAA
                     EIVRDEANRAGQFFVTGRWLGGTLTNFRTMKNGIDRLRAIEQQVEDGTINALRKKEAL
                     SIRREAEKLEKYLGGIKMMTSLPSVLFVIDPHNEHIAISEARKLHIPIVALTDTNCDP
                     DLIDFVVPGNDDALRSIKLITSRIADACIEGSQARRDFLQHDGAQAPDRASAEGVQVE
                     FARKRASAPAAPAAPAAPEGKE"
     gene            complement(26529..27116)
                     /locus_tag="VFG22_04285"
     CDS             complement(26529..27116)
                     /locus_tag="VFG22_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415489.1"
                     /translation="MNRIPRLSYLDPLDAVWLTTAGRIGFRVERSADVYASTDGQGVM
                     IIGDPSTLDADDCLAQMILHELCHSLIEGQESLAVPDFGLDNESERDLVREHACLRLQ
                     AWLTGEHGLRQALAPTTDFRIYYDTLPEDPLGDVDDPACALASQGAARSREGPWAPHL
                     QEALKTTATILRAARRLGAEDPEADTPPIWSKLED"
BASE COUNT         5454 a         8375 c         8500 g         5017 t
ORIGIN      
        1 agcaggacgt agaactcgcc tcgctcgaag ccgtgctcac ggaggtatcc acctgcaaga
       61 agggcggaga gtcccgcacc tactgcgatg accacgtcga agaagagccc catgcggtcg
      121 acggcgaggt atcgcgtgac gagggcagga gcgggcgcga tgtcggcgct cacgagcatc
      181 gccccagcca cggcagcagc tgcaaagagc gagaccgtgg tcacgatcgc gatcccggca
      241 ttctccttgg tgaacgcatc gactagcatg atggccagcc cggccgcgac gagtatcagc
      301 aagggcgaca ctcccatgag tgagctcatc gccccacctc ctcgtcattg ggctcgacca
      361 tggccaagcc gccggcggct tcgatccgct gcctgagctg gtcgaagtcg accaagtgag
      421 cctcggacgt gcggcggcct tcgtagtagc gaacggtgaa gtctgtcacg aacgaatcga
      481 ccgtagggtt catcttgtcg aggaagaact tcggatagag tccgatccaa aagaccatca
      541 cgatgagcgg cagcaaggag acgatttcgc ggcggttgag atcgggcaac gactcgtttt
      601 cgctgtgcga aatcttgccc cagaagatgc gaaggacggc gtgcagcatg tacacagccg
      661 cgaagatcac gcccgtcgca gcgagaagcg tcatgatgaa ccaggacgag ccgtagccga
      721 ttggggtgcg cgagaaaaac gagccagcca agatcatgaa ctcgcctacg aagccattgg
      781 tgcccggaag gccgatcgag ctcatcgtga tgatgacgaa caccagggtg tacatgggca
      841 tgactttcgc gaggccgcca aaatcatcga gcatgcggct gtgccggcga tcgtagatca
      901 cgccgacgag aagaaagagc gcgccagtcg aaatgccgtg attgatcatc tggagaatgg
      961 cgccggagat gccgtgccgg ttcatggcga agaggccgag caagatgaag cccatgtggc
     1021 tgacggagct gtacgcgacg agcttcttga ggtcgcgctg cacccaggcg gcataggctc
     1081 cgtagatgat gccgaccaca ccgaggccgg cgagtaccgg gccgatcgcc tgactggcaa
     1141 ctgggaagag cggcatcgcg aatcgaatga aaccgtagat cccgagcttc aggaggaccg
     1201 cggcgaggat cacggagcca ccggttggcg cctgaacgtg cgcatcgggc aaccaggtgt
     1261 gaaatggaaa catcgggacc ttgatcgcga aggccagcgc gaaggccgaa aacaataggt
     1321 attgcggcat tgcgggcaag accaagctcg aaagggcctc gaggtcgaag ctgtagtgcc
     1381 ctgcgagctc cttgtagctg gccaccagat agaggatggc aacgagcatg agcaagctgc
     1441 cgaccatcgt gtagaggaag aacttcaccg ccgcgtaaac gcggtccttg ccgccccaga
     1501 tgccgatgat caggtacatc ggcaccagca tgagctccca gaacacgtaa aagaggaaga
     1561 ggtctagggc tacgaacgcg ccgagcatgc cgagctcgag gagcaagaag gaaaacgcgt
     1621 actccttgat gcgcgtcgtg atgctccccc aggacgcgta gagcgcgatg ggcgagagca
     1681 gagtcgtcaa gatgacgagc cagagcgaaa tcccgtcgac gccgaccttg tacgagatac
     1741 cgaaagactc gacccaggtc acctgctcga cgaagtgata accggccccg gagtagtccg
     1801 cgaggaccag ccgaagactg atgatcagct cgagcagcat tgcgccgatc gagaagcgct
     1861 tgatgccgct ttcccagctg cgcggcatca ggagcacgat gatcgcgctg atcgcaggga
     1921 gtgcgaggag ccaggtgagg tactgggtgg tcatcgaacc acctcatcgg cggggagcat
     1981 caaggatgcg tgggaaggcg cctcgactcc gaacggcagc gcaatttcct tggtgtcgcg
     2041 acggaggttg ccgaaggcat tgcgaacttc gacggtggct tcgaccaggg ggcgggcaaa
     2101 gacgcgggcg ggcccgagac gtccgtcctt gcccagcggc aaacggattt ccttgcttcc
     2161 cgagagcttg gtcttggcgt gcggcttacg aaacaggagg tcggttccgt cgatgcgaat
     2221 ctcgaaccct tcgtcctggg gcatgatctg ctcgatgggg accacggact cgagcggaag
     2281 gggcgaccag tcgtcggtga ttggaatccg acgttcgatg cccgcgtgga cgttgacgag
     2341 aaacacggcg atgccgcgga tgtcgtcctc ggcgtattcg aacgatgttt ccggagaccg
     2401 gtcccaggcc gtctcgaaat ctccgtccga attggcatcc cagcgatact cgtagccaag
     2461 tcccccgggc gtgctgagcg cgacgcgcgt gccctcgaag acggtttcga tccgcgagtg
     2521 cggcgtccag aaccaggtca tgaccgcaac gacgccgacc agcatcgcgg tgccgtacat
     2581 ctggacgacg ccattctgca tgcgggcaaa taggcgtccg gtctcgcgga cgttgaagct
     2641 cggcacgcgg gtcatcagcg cgtcgacgaa ggtcatgtcg gcctcacccg ctaccgtcgc
     2701 aatcttcttg atcgggttga cgatggtcat cgcgtagagc tcgtccaccc gccacttgtc
     2761 gaaggccagc tgaaagaggc gcttcggcat tctctcggcg agagcgtcgt ggctcttgtt
     2821 gcgatagagg acccaggcgg cgctgattcc aatcgccatg gctccgaacg cgagaccgga
     2881 cgcaagattg acgatgtgca tttcctccgg aaccggctgg ccggcaaccg aggactccat
     2941 ccaatgcccc caccagctgt agagatgcgt cccggtgatc ggcatgacgt gcggtaaccc
     3001 gagaaaacca accagcagcg caccgatccc gagcaccacc agaggaatcg tcatactggg
     3061 cggcgactcg tgcggatgcg catcgtaagg atggtcgccg gactgatcgg cgctccggta
     3121 ctcgcccgtg aaggtgagga aatagagccg gaacatgtag aaggccgtca ttaccgcggc
     3181 cagggtgagg ccgatcaagg tgaaccagcc gaccgcggtc atcagcgcat cgtcggggcg
     3241 atagatctga gcggcagcac cgaggagaat ctcgtctttg gaaaagaagc cgctggtgag
     3301 cgggaagccc gcgattgcaa gacagctcgc gaggaatgtc caacgggtaa tcggaaggac
     3361 tttcttgaga ccgccgagct tgaagatgtc cgcgtcgccg tgcgcatgaa ccgcatgcat
     3421 caccgagccg gcaccgagga acaaacaagc cttgaaaaaa gcatgcgtga agacgtggaa
     3481 gaacccggca gagaagaagc cgaccccgac agcggcgaac atgaaaccga gctggctcac
     3541 ggtggagtag gcgagaatct tcttcatctc tcgctgaacc acggcgatcg acgctgccag
     3601 gagcgccgtg agcgtcccgg tcaccgcgat gatcgacatc gcgacgtcag aggtgatgaa
     3661 cagcggtgag agccggcaac agaggtagac gccggccgtg accatggtgg cggcgtgaat
     3721 gagggcggaa accggcgttg ggccggccat ggcgtctgga agccaaacga agagcggaat
     3781 ctgtgcgctc ttgccggtgc aaccgaggaa caggaacacg caggcgacgg tggccaggct
     3841 cggggccacg ccgaatatcc aaatcgggaa atcgggacgg aactcgccgt gcatggcggc
     3901 ctggttgatc tcggcgaact cgaacgaacc ggcgacgccg acgaggatga acatgccgat
     3961 caggacgccg aagtcaccga ttcggttgac gacgaaagcc tttcgaccag cagcggcata
     4021 gtcaccgttc tcgtaccaga acccgatgag gaggtacgag cagaggccga cgccttccca
     4081 gccgacgaac atgaccggca agctgcttcc gagaacgagg atcagcatcg aggccatgaa
     4141 caggttcaga tagctcatga acctcgggta gccctcgtcc tcccccatgt agccaatgct
     4201 gtagaggtgg atgaggccgc ctactccggt gaccacgagg gtcatcaccc cgctcaagga
     4261 gtccatcaga aaacgcacgt tgatcggaac gacgttgccg cccgggagcg tcagggagaa
     4321 ccactcgtag aactgatagg aaagtactgc gtgggcctct tcatggggca ggccgtaaag
     4381 ctggatgaac gaggtcacgg ccagcgcgaa tgagaccgca accgatccga ccccgaccga
     4441 ggtcacgatg cccttgggca tgtaccgccc aaatagcccg ttgatgatcg cccccgccaa
     4501 agggaaaagg atgatcaaaa ggagcaggag ctgctgatgg gcttcttcgt gcatgggttc
     4561 aatacttcag cagggtcgcg tcgtcggtat cgacggtctt tttgattcgg tagtacgcga
     4621 tgagaatcgc caagccgacc gccgcttcgg ctgcggcgac cgcgatgacg aagaagacga
     4681 agacctggcc gtcgtggttc gccgggtaaa cacgattgaa ggcgagcaag accacgttga
     4741 ccgagttgag catgagctcg atcgacatga gctggatcag catgttgcgt cgggtgagaa
     4801 accccacggc gccgatgcca aagaggaccg tcgcgagcgc cagatatgtg ccgatcccca
     4861 tcacgtccta ctccgtccgg ctccgagcta tggcgatcgc gccgatgatg gcgacggtga
     4921 gcgtgatcga aatcaactcg aagggaacca gcgcctgctt gtacacggca gtgccgaggc
     4981 cctgcacggt gccaaagtct tcggagacga cgccccaggg cacatcgaaa taggcgacgg
     5041 aggtcgaaac cgcgagcacg accccaatcg tcaaggcaat gctcagggca cgcgaggaca
     5101 cgcgcccctg cattgcatcg acctccgcgg ccggaccgat cagcatgatc acgaacacga
     5161 acaagacgac gacggcgccc gcgtacacga gaagctgcaa cgctgccagg agctccgcat
     5221 tgagtgtgag aaacatgcct gcgagcgaga tgatgtggat gagcaggccg atcgctgcgc
     5281 ggagcggacg cctcgaagag atggtgatca gtgcgccggc catcgcgagg atcgccgtgg
     5341 agacgaaaag aatggttccg ggaccgatca tgcgcccccc attaccgtgc tgcgaacgct
     5401 gtcgtcgtgc cggacgtcgt cgggaactcc cctgagggcg gcctccatga actcttcgcg
     5461 gaacttttgg accatgccca acatcggcat cgccgtgcca tcggcaaagg cgcagatggt
     5521 gttgcccatc atgttgttgg aaatattcac cagcaggtcc acgtcctcgg gcttcccgcg
     5581 ccccgcgcag agattgtcga gcacgtcgac cagccagccg gtgccctcgc ggcaaggcgt
     5641 acactggcca caggactcgt gcttgtagaa acgcatgagg ttgcggcagg cctcgaccat
     5701 gttgacgctg tcgtccatga cgatggcgca gcaggttccg agcatcgtgc cgagcgcccg
     5761 gtaggtgtcg acgcccatcg gcacgtcgat ttcgctcttg ccgtgccact tgtggagcgg
     5821 atgatcggcg ttgggcgctt ccactttccg ccgtgggtcg agcaccggtg tcgaggagcc
     5881 cccggggatg accgccttga gagggcgatt cccgagcatg ccgcccgcgt agtcgtcgat
     5941 gagctctcgc atcgtgaggc cgaccggcgc ttcgaagagg ccagggtttt tcacgtgccc
     6001 gctcaccccg tagatgcgca gtccgccgtc gcccggaagt cgcccgagcg ctgcccaggc
     6061 gtcgccaccc atttcgacga tatcgggaac ggaggcgatc gtctcgacgt tgttcacgat
     6121 ggtcggcatc ccgaaggcgc ccttcacggc ggggaatggc ggtttgagcc ggggctctcc
     6181 gcggcgtcct tcgagagagt tgagcagagc ggtttcttca ccacaaatgt acgcgcccgc
     6241 ccccgagtgg acgtagacct cgatggaatg gtcgatgcca aaagggcgct ggccgaggta
     6301 cccagccttg cgggcatcgg cgatcgcctg ctccagtcgg ccgatcgaga acgccaactc
     6361 accgcgcacg tagatatagg ccacgtgcgc gtcgatcgcg aagcaggaga tgatcgcccc
     6421 ctcgacgagc cggtggggat ccaaatccat gatgctgcga tccttgaaag tgccaggctc
     6481 gctctcgtcg gcgttgatga cgaggtagat ctcctcgccc tcttgcttgt tgaggaagcc
     6541 ccacttcacg ccggcaggga acccggcgcc gcctcggccg cgaatattcg ccttcttgac
     6601 ctcatcgcgg agctcggccg gctttcgagt cgtgagcgcc tggcgcgcga cgcgatacgc
     6661 ccctgccgct tcggcattgg cgagcttcca gctttccgcg atgccatagc gggcggtcag
     6721 caatggcttg atcggctggc tcatgagtcc tcgtctctcg tatcgcccaa atctggggtc
     6781 gcggccgctg gtggcggttg cgacgcgaga cggacggcct ccttgctcgg tggaggcgga
     6841 gcggaggtcg gacttccgcc gcgctggcgc ggggagccaa acctcgcgaa cgtcgaaaac
     6901 tcggcggccg cagcttcgcc gccctcgttc gcgtgggcgt cgatgattcg gtccaggcgc
     6961 tcgacatcga ggttttcgta ataaaagtcg ttgatctgga ccatcggggc ttgcccgcaa
     7021 gccgcgaggc attcggcctt gagcagcgtg aatttgccgt cggcgctcgt atgacccgca
     7081 gtgcagccga gcttctgctc gaggtgatcc tgaatcgctt tccccccgcg aagatcgcag
     7141 gaaagcgtgc ggcagaccca gacgacattc tcgccaaccg gctcgtcgaa gaacatcgtg
     7201 tagaacgtca cgacgccttt gacgatcgcg gtcgagagct cgagacgctc ggagacgtat
     7261 tcgacgacct cagggctgat ccagccgttc tgtctctggc agagccacag aacaggcaag
     7321 agcgccgctc gacgctcggg atagcgggtc aaaatctcgt cgacctgttg ttcgcgttcc
     7381 cgcgtaagcg aaaaaaccat ggcagagcgc ccggacgcta ggcccgcggg gctcccctgt
     7441 caaggtcggc ggaacagtgg taactcgggc ccaagatgag tgaaaacgcc gcaacgccgc
     7501 cctcgaagag cgtcgggctc tgggtaggcc tcggatgcct cggtgtcctg gtcttctcgt
     7561 gctgcttgct gacattctgg gcgcagtcgt acggcttgcg cttcgttttg gggcaggatg
     7621 acgggaccaa ggtctggttc tctcggatga tcttggtcgg tgcgctcgaa ggaacgcgca
     7681 agacgtgcag cgacggcgtc gtcagcgaag acacgcttcc ctggtttcat ccggagctgc
     7741 ccgcggagtc gcgaaatcta atttgctcgc tcgatgagac gactctccaa gcgctcagca
     7801 cggcagaacg ggcatcggca actccattgc tacgaaccga ccgcagcgag cttgcggcgc
     7861 gcttcgggat ggacgcagct cagtgtttcc aacattcgac tgatggactc acggccgtcg
     7921 gttgcttcga tacggaaagc ggccccggat ccattccgta tcagatcatc gatctcagag
     7981 tcactcagcc gtgacgtcga tcggcgtgcg gcggtatgcg acggtgaggc cggcgcttcc
     8041 tggatagtcc ctaggccgaa gctgcagaag aacgcacggc tcggagccct cgtccgtcac
     8101 gcagtcggtc agagccgcaa aggtgcggtg ctctcgcagc ccgtgccact tcactccgac
     8161 tgggatctcg cgctcccgcg gatgacgaag cgaatccacc gacccatcct tctcgagaaa
     8221 caggatctcc gcttgcaaga tgttggggtt ctcggaaaac gtgtagtcga gcatcctcca
     8281 gaagagcgtc tctccgtgct ggagctgctg ttcggtgacc tcggcccctt ccgcctgcat
     8341 gcgttcgagc atgggcttca gattctccgg gagcgatcct tcgtcccctg ccatcgcctt
     8401 tgcgagcaag cgtcgacgct cgacgtcgga gcgcagctcc agaagactca acgccctcga
     8461 tcggagcgcg ccgaagtcct cgatataggg ctgcgcagcc cgaactgcct cggctcgtcc
     8521 ggtgcaaccc atcaagaacc ccgaaagcac aacgcccagg ggcagccatc cgaagccacg
     8581 gcgcatggcc cctacagtag tcgacgagcc cggcaagccc aactaaagtt cgcccgatgt
     8641 ccctcatcga gtttcgcggg gttcagaagg cattcggttc aaaggtcgta tatcgcgacc
     8701 tgaacctcga agtgcgaggg cgtgaagtgt tgacgatcat cggtggctcc gggcaaggca
     8761 agagcgtgat gctgaaaatg ctgatcggcc tgctcagcgt cgacggtggc gcgatctact
     8821 tcgatgacca acaaatctcg ggcatgagcg aggcgcagta cgccgccgtt cgtcgccgcg
     8881 tcgccatgct ctttcaagct agtgcgctct tcgattcact caacaccaag gagaacgtcg
     8941 cgtacgggct gcgagaacaa ctcgacatga gcgaaaaaga gatctcggag cgggtgcggg
     9001 agtctctgac gtacgtaggg ctgcccggga ccgaagagac ctggcctgcg gacctctcgg
     9061 cagggatgaa gaagcgtgtt gcgttggctc gggcgttggc gatcaggccc gaggtcttgc
     9121 tctacgacga gcccacgaca ggactcgatc cgatcaacgt cacgcggatc gcggacctga
     9181 tcgagtatct caacgagact ctcgacgtga cctcggtcgt cgtgacgcac gacatggaca
     9241 ccgcgctcgc gattagcgac tgcattgcca tgatccacga gggattcgtg attttctctg
     9301 gcactccgga cgagctgagg gcctctgaca acccacaggt ccgcgacttc atcgaaggca
     9361 acgctccggt cgacgaggac acccaaactc tgctaaggag tgctgggtaa ggggctctgt
     9421 tgggcgacaa atgaagacga ctgccggtac tcgccttcgt gttggcattt tcgtgctggt
     9481 cgctctggtg atcggcgcga gcgtcgcgtt tgcgatcggt gcacaggaaa acatattctc
     9541 ttccaagacc gagtactacg ccatctttca cgacgtcggc gggcttcaga agggcaacgc
     9601 ggttcgtgtg gcgggggtca acgtgggctc ggtcaccgag gtcgtcatcg gcgacgacgg
     9661 ccgcatcgag gtctacttca gaatcatcga ctccgcgacg cacttgatcc ggggcgagtc
     9721 gggggcactg cttgacagcg acagcccgcc atcgagcttc gcagcgatcg gtagcaaggg
     9781 cctgctggga gaccgactga tcgaaatcag cgtcgggtcg cacgagtacc ccgaatggga
     9841 cccggatgaa ccgattcccg tgtccgtcgg aggcggcatc atggctttgg ctgagcgcac
     9901 tctcgtcgag gccgaaggga ccgcgcacaa ccttcggctc gccaccgacc cgttctccga
     9961 ccaggagttt tcgaacgacc taaaagagac ggcgcggaac ctggcaaagg cgaccggcct
    10021 gatcgcgagc ggcgatggaa cgattgggcg attgatgaac gacgccgagc tcgggcgtga
    10081 cgtaaagggc gccgtgaagg agctccgcgc ggccggcaag caggtggccg ccctgagcaa
    10141 gaacctcaac aagatcaccg aagacatcca gacgaacgag gggacggcgc acgcgctgct
    10201 ctatggtgag gagggcgctg acgcgattcg caacatccgc gacaccttcg cccagctcaa
    10261 cacgattctc accgacgtcc gggagggcga cggtgcggtg aatcaactga tctacgggga
    10321 gctcggcgag tcgttcatgg acaacctcca gagcgccagc gacgatattg cttttctggc
    10381 caaagaggcc cgggagggca agggcacgat tggcgcgctc ctcaccgacc cgtcgatcta
    10441 cgaagacatc aagcgcttgg tcggcgacct agagcgcaac gacattcttc gcgccttggt
    10501 tcgctactcg atccgccgcg acgacccgcg cgagccggtc gaggttctag aagagtagct
    10561 cgatgcaggc ctatctcgac ttgctccgga aggtgttgaa cgatggcgta gcgcgagacg
    10621 atcgaacggg aaccggaacg agaagcttgt tcggctacca gatgcgagtc gacctgcgcg
    10681 acggcttccc ccttctcacg accaagaagg ttcacctcaa gtcgatcatc cacgagctgc
    10741 tctggttcgt gcgcgggcaa acccaggtga gtcacctaca agaggtgggg gtttcgatct
    10801 gggacgaatg ggccaccgcg gaacagaccg ctcgtttcgg tcgcaaggaa ggtgacctcg
    10861 gacccgtgta cggacaccag tggcgcaact tcggggccac tgaacgagca gatggcacat
    10921 atgaacgcga tggcttcgac caactctcct acgtgctcga ccagatccgt gacaatcccg
    10981 atagccgccg gctgatcgtt tccggatgga acccgtccga agcagagcgc gtcgcccttc
    11041 cgccttgtca taccttgttt cagttttacg tgagcaacgg acgtttgagc tgtcagctgt
    11101 accagcgcag cgccgacgtc tttttgggtg ttccgttcaa catcgccagc tactcgcttc
    11161 tcacgatgat gatcgcatcc gtcgcgggct tggagccggg agacttcgtg cacacgttcg
    11221 gtgacgtgca cctctacagc aaccacatcg agcaagcacg ggagcagctg tcacgtcaac
    11281 cgcgcccgct gcccaaaatg tggatcgatc cctccgtcca aagcctcttc gattttcgat
    11341 tcgagcactt tcgtctcgag gattacgatc ctcatccgcg catcaaagcc ccggtggcgg
    11401 tgtgagcgac acactggcag gcccagcccc cctcgcgttg atcgtggcgg tggctcgcaa
    11461 gggagtcatc ggaaaggacg gcgggcttcc ctggcacgtg tccgaagacc tcaagcactt
    11521 caaggcgacg acgagtggtc acgcgatcat catggggcgg aagacctacg actcgatcgg
    11581 caggcccctg ccgaagcgcc gcaacatcgt ggtcacgagg cgaatcggcg ccgagatccc
    11641 gggctgtgaa gtcgctggga gcctcgacga agcgctggcc ttggcccgga ccagcgaccc
    11701 ctgccctttc atcatcggcg gcgcgagtct ttacgaagaa gccctgccca tcgccaccga
    11761 gctctacatg acgaccatcg acgagaacgt cgaaggcgac acttatttcc ccgaagactt
    11821 gccggatttc gaagaggtcg aggcccgagc cggacaaacg tctgggatcg tgttccgcgt
    11881 cctgaagcgg aagtcgacga gaacgcgttg agtcgatcgc tggtagcgct gatctagcgc
    11941 ccccccacct gactcttcag aaccgtgtag gcccgctgtc cttgagaatc ctccgccaac
    12001 atcttcggca cagacccgct cgccagcgga gcctgattcg gagcgggcgc atttgcagag
    12061 cccccgaggc ctttcgactg tgccgcaaga tgtccatcga gctcctcttc gcggaacggt
    12121 tccggatcgc cctcgggccg cggctgctcc gccaataggt ccggggaaat gccttccgct
    12181 tgaatggagt tgcccgaggg cgtgtagtat cgatacgtgg tgagcttgag cgcgctgccg
    12241 tcaggcagct cgaacacgtt ctggacgctg ccttttccga acgtccggac accgaccaac
    12301 tcggcacgat ggtgatcatg cagcgcgcct gcgacgatct ctgccgcact cgcggtgttc
    12361 tcgttgatca ggaccacgat cggccacttg gggcgggtcc ccgctcgtct cgccgagtac
    12421 tgtccgacca tctcgccccc gcgtccacgg gtgctcacga tgacgcctga cgcgaggaac
    12481 tgatcgctca cccagaccgc ttcatgcagg aggccgcctc cattgtctcg aagatcgatc
    12541 agaagtcccc tcacaccgtc gcgttttcga agcttcatga ccgcttcgtc gagggcgtca
    12601 ctcagcattc gcgccgttcc atcttggaac gccttgatcc gcaggtagac gattccgtcg
    12661 cccaacagct ccatgtcgat cggatcgaga tcgatgaagg cgcgggtgag cgttcgctcg
    12721 agcgccttcg cgaagccttc gcgccgaatc gagactttca cgctcgtacc gggctcccca
    12781 cgaatcttct gcaccgcatc atataggcga agatcacgag cgtcttcgcc ctcgatggtc
    12841 aagaagcgat cacccgggcg tagacctgcc ttggccgccg gaccaccctc gaacacggaa
    12901 agaatcgtga gccaaccgtc acgtgtcgac acttcgacgc ccacgccggc gaagcgtccc
    12961 tccgcgtcgc tcttcagtat gtcgtactcc acgggatcca aaaacacgct gtgtggatcg
    13021 agcgaatcgg caaggccacg aatggcaccg tatacgagac tctcttggtc gaccggctcg
    13081 atgtaactca attcgacgta agcgagtgcg cgcgcaaata tcgaaagcgg ttcaaatggg
    13141 cttcgcctgg ctggcgcgtt agatgccgtg gcagcaacga gcagtagcgc aagcgcgccg
    13201 acgacggtta gacggagtac attgcggctg gatttgtgac ggaacatggg cttcagacct
    13261 ttgtcattga cactgctacc atccctgggt aggaccacca gatacttgaa cggggcgcgt
    13321 tcgctacacg gcacggcgtc cggatggagg attcctcacg atgtccacga ctgacaagcg
    13381 aaagcaaagc ctctacttcc cggagtcgat gctacacgac atcaagcacg aatcggcgag
    13441 gttggatcgc tcgctctcgt ggatcgtcca gcgctgcgtg aagatcgctc tcccagagat
    13501 ccgcaagttg ccctcggtca acgacatccc ggacagtccg gacgacgacg gcaagaagta
    13561 agcgcgtcta gcgtgcggta cgagcgcgat ggcttcgtcg aagcggagga tggcacccgg
    13621 ctgttctacg aggagctcgg aggaggtaac ccgccgatca tcctgaccga tggaatcggc
    13681 tgtgatgggt tcgtttggcg ctacctcatg ccaaaccttg ctcgtcatca ccgagtcgtt
    13741 cactggcact tccgcgggca cggacgaagc ggccctccgc gcaatcccga gcagctgtcg
    13801 atcgaggacc ttgcgcgaga cctccgcacg atttgtgacc atctcgaggt cgagcgcccc
    13861 gtgatcgccg cccactccat gggcgttcag gtcgccctcg agtactaccg gcagaatccc
    13921 gatgacgtgg cggcgttgat cttgatgtgc ggaaccttcg ggcgcatcac gagcacgttt
    13981 cacggtaccg acctgctcga ccaactcttg cctagcatcg cgcttggggc gcgcatgttt
    14041 ccaaacgtag cgcgtgcaat ctgggggcgt gtgccggccg cgatggcgtt ccgcgtcgcc
    14101 tgtgcgggtc gcgagctcga tgccgagcgg attcaagaag aagactttca gcgctactgg
    14161 gagcattcct cactgatgga cccggaggta tttctcccca tgcttcaact ggcggggcag
    14221 cacgacacgc gcgggtttct gccggaggtg gctgtcccga ctctcgtcgt tgcggcggag
    14281 cacgacacgt tcacacccat ggcgcttgcc gaagagatgg cgggagcgat cccggacgcc
    14341 gagctcgaag tgatcgaaga agcgagccat gcggcaccgg tcgagcaacc cgaccgaatc
    14401 acggagcgca tcgaggactt tctatcctcc cggctgggcc tccagcgggc tcggcctcag
    14461 tagtcggcgc ggaagagttg ctgccccatc aggacgaaag tgctgggcgg gacgtaggat
    14521 tcattcaaca cgcgtacgaa cgttccgttc gagcttccga ggtcggtgag aaacacgtgc
    14581 ccgacctccg aatgaacgcg gcagtgaaga ccggaaacgt agccgtcttc gggaaatacg
    14641 atgtcgccgc gctcacgacc gagatggatc ccctcgggcg gaatcggata actgtttccg
    14701 gtggtctgcc gaccgatcaa gagagaaatc cggccgaggt accctcggtg cgggctcccc
    14761 atgtgctggg tgccgtcagg agctgggtca ggggcgaacg gggcctcgaa acgcaagatc
    14821 tcttgcccca cccgaaaaat gcttccgtcg aagagctcga tgggcacttc gggctcgatt
    14881 cgaatgtaca cgccgttgag gctttccatg tccctgacga cgagctgatc gctttcgaaa
    14941 cgaaactccg cgtgctcggg tgagaggtac gcatcgttgg cgaagatccc gccggtgctt
    15001 cggccgatca ggctcccacg cgcggacaaa gaaaacgctt cgccttcgga tccgtccggc
    15061 tgaatcaata tcagttcggc gcgcgccgtg gctggcagcg aagtctcggc cacggcgggc
    15121 gccgagacgc gttcggcggg caggtcggcg gatgcaccat ccgaggcctc ggcatgcaga
    15181 tcatgcccgc agacatgaca gaagcggaag ttctcaggaa cctcggcccc gcaatgggag
    15241 catggagccg tctcgtcgcc cagcggggcg agctccggaa gaggtgcatc ctcggctgta
    15301 agcggctccg gatccatctg aagggcctct gcctgggcgg ctcgcgcggg gggcgcattg
    15361 gtggtccggc ccctcgatgg ggtcccgcca ggcggggtgg ccgccgagaa cgacttcggc
    15421 tgcgacgaat gtcgcggaag ctcggctcca caccccagac agaacttgta gtggtcctgg
    15481 ttgtccttgc tgcatcggga acagacgatc acgccgtcac ctccactcga agcaggttcc
    15541 tgcccaagaa cacgtaatcc ccatgactca gctcccgttc accccgaatt cgaacgtagg
    15601 tcccgttctg cgagttttcg tcgaccagcg ccagggactc tcccgacatt tcgactcgag
    15661 cgtgccgggg cgacatgtac acgtcgtcgg ggaagttgat gtcgcagtct tcgcggccga
    15721 tttgcacgga ctgctcgcgg gcgcagcaga cgatcccgtc catgccgcct tcgagcacct
    15781 gggtaatccg aaacgcgctg gggcgcctcg gcgaagcgta gaaataagtt tgatctgctt
    15841 cgggacccga cgtgtccttc ggcgtcgcgt cgacgcggaa gagctgctgg ccgcacatga
    15901 aatggtcgcc gatttgaatt ggcacggttc ctcgtatgcg gatgtagacg ccgttcaagc
    15961 tcccctcgtc acgaacgacg agcttttctc cgcgatagag gaagttcgcg tgcgaagggc
    16021 tcagccagtt gtcgctgggg aatggaatct ggtcggcttc gcggcccgct acgtgctcgg
    16081 tggcttggag cagatagctg aggccctcgc ccatggcttc atcgccgcga accacgatca
    16141 gccgcgcctt gcccggcatc tgcagcgcgc cgaagaagcg cgtcgcgagc gtctgcgcct
    16201 cggcgggcac attggcccca caggccccgc agaacttgtg tccggacgga acggcgcttg
    16261 cgcactccgt gcagacgtaa ttccgagctt gctccatgcc cctcgttccc ctcctcgggc
    16321 cactctcgga gcggccaacc tcccggcagt tgtaggccat cgaatcaccc ctagtcaatt
    16381 gggggtcgac ggggcgtttt cggaaagcaa tgcgcctttg cttgtgctcg gcgtgcgact
    16441 accctctcgc cgatggagct cctcgcaggc gcattgttgg agcggccccc gggcccgaag
    16501 tacacgtcgg agcttcgttt cgcggagctt tcaccgcagg ctcccctccc tagaccggac
    16561 accttggcca aatggcgcga aaagcttccc aaaggatttg agctggctct tcgcgccccc
    16621 gatgtatgct ggcaatcgcc aactggcccg ctgaggccga gcgaggagct cgatgccggc
    16681 ctcgcgtggc tctccgaagc cgcagacgcc ttgcaagcat cgatgctggt cgtcgcgacg
    16741 aacgccgcag tgacgaccgg agcgcgagac cgcgagcgcc tgcgggaata tttcgcccgg
    16801 attccgagga acgacgcacg gctcctggtc tggcaagcat cgggcctctg ggagcatgaa
    16861 ggggtacagt cgatggcgcg aaagctctcg gtgctcggcg ctttcaacgc tgcggacgat
    16921 ccggcgccag atgatgcaga gatcgtttac gcctcgttgc gtgccgaagg gctccgtcgt
    16981 tcgttttccc atgcacatct cctcgacgtc ctcgacaaga tcgaaggctc cggcgcatcg
    17041 cgcgcattcg tcacgatcga gtcggcacaa tcctttcgtg aagcgcgcct cctacaggcc
    17101 ctatccgaag ggcgagagtg agcgcgtgga atgcaagaat cgcccgcgtt gaccaacccg
    17161 aacccgggct actgagtctt tcgttccgaa ccgagggccg aaacgaagcc ctagtcctcg
    17221 taacgctgcc cggatcactc gacctcggag tggtcgacga acggccgcgg ggggcgagcg
    17281 cagggccggc cgtcactcag cttcggcgcc atgtggaggg atcgcggatc gaatcggtcg
    17341 acgtgagtgg gcgcgcgatt cgcctttcgc tgagccgagc cggaaccaat cgcttcctca
    17401 tcgcggcgcc gagcaagccg tacggtgcct ggtggctctg cgaggaagac ggagccctcg
    17461 tgcttcgctc acccggtgcg ccggcctcca tgccttcaga gcaagagcac ttgcgaccca
    17521 aaggcctgga cgagctgcgc gtccgggggc ttgccgtcct gcaggcccac cgctcgaccc
    17581 gaaagcaaca gctcgagcgg cttctcgccg ctcagatcaa gcgtctatcc aaaaaacgcg
    17641 atgcgattct cggtgacttg gaccgggctg caaaggccgg cgagttgcac gaaaaggcaa
    17701 gcctgatcct ggcccatgcc tcggaaattc cagaacgtgc ggcctatttc gatacccaga
    17761 cctgggacga cgagccgcgg gcaattcgca ttgagctgga tccgagaagg acgccgaccg
    17821 agcttgcgca ggagctgttc aagaagtcga agcgattcaa gcggggttcg gacgtggcgc
    17881 cgcaacggct cagtgcggtc gtcgcggagc tcgagaagct gatcgaagtg cgcgaccgac
    17941 tcgaggacga aacagctgag gagctggcga ccaagctcga agccttgggt gtgccgatca
    18001 cggcccccaa agaggaggca cgaaagcgcc gacaagccgg cgagcgccta ccctatcggg
    18061 agttcatctc cgtcgatggc tcgccgttgc tggtcggccg aagcgccgcc gacaatgatc
    18121 ggctcaccct tcggcatgcg cgaccgtacg atctctggct tcacgcccgc ggtgtcacgg
    18181 gcgcgcacgt tctggttcga cttgcaaagg ggacccaatg tccaccggag acgctcgtcg
    18241 atgcagcaac gctggcgggg cacttcagcg acctccgcgg cgaggcaatc gtcgatgtgc
    18301 tctacacccc gcggcggttc gttcacaaaa gaaaaggtag ctccgtcggc tcagtcacac
    18361 tcggaagcga gaaggtgatc gctctgcgag tggagcccgc gcgcctcgaa cgccttctca
    18421 aaaacgaacg acgcgcgacc tagcccttaa atggccaacg ccccagccga gcggccaggg
    18481 cgctagcgga cgaacacccc ccattactaa gaggggccgc cacgacagac tgcgcgtagg
    18541 ccctggaagc ccagggcctt aggcgagggg ccaccagagg tgaccccttg gtcgacccga
    18601 aggtctaccg cctagaaggt tacttcttct tgcgagccac gcgcttctta gcggcgcgct
    18661 tcttcggtgc agcctttttc ttggttgctc gcttcttagt cgctcgcttc ttagttgcgc
    18721 gcttcttagt cgctcgcttc ttggttgctc gcttcttagt cgctcgcttc ttcacagcct
    18781 tcttggctgc acggcgcttc acggccttct tcacagcctt cttcgcctta cggcgcttca
    18841 cggccttctt cacagccttc ttggctgcgc ggcgcttcac ggccttcttc acagccttct
    18901 tggccgcacg cttcttcgcc ggcctcttct ttttcgttgt tctctttttt gcagtcgcca
    18961 tttcgacctc ctccaaggtg ggtcaatatt gcaggtgcac gtaccgtatt gtcacgtcac
    19021 ttcacgtgtc aagcggaagc gcacatgaat tatacataac tttagtctat ttgagccaaa
    19081 tgttttgcaa cgtcggcttt caacactctg aaatcattta tgaacattag attatcagac
    19141 caggctgaaa agctgaagaa agttcggaaa ccgccgcttg gcgaaagatc tgacgtccag
    19201 agagcagatc tgttcgcggc gtcttgggct cctagatcgc gttgacccgt atgcgtgctg
    19261 ggactacatt tcgcccctgc tggggaagta cccggctgca ccgaaagggc ggggacgcgc
    19321 gaagtgcgac gctgaggccg ggaatcatca cgacgcagcg cccgttcaac ctgacgcctg
    19381 ccaatcaggc ggaagggaac atttttggcg agagaaggag tcccgatgca agcgggaaca
    19441 tacgaattca aggttggcga caaggccatc tatccaaagc aaggcgtcac cgaagtcgtt
    19501 tcgatcgaag tgcggaaaat cggtacggct acgatggaat tttacgtttt gaggccgctg
    19561 gatgcgaaga acaacgatcg catccgcgtg gccgtcaagc aggcaaagaa ggtgggcctc
    19621 aggccgccga tctcggagac cgacatcaaa gagatcttcg cgctgctcgg cgaacagccc
    19681 gtcgtattcg acaatcagac ctggaatcgc cggtatcggg gtttcatgga caagatcaag
    19741 acaggttcag tcttcgatgt ggcggaggtc atgcgcgatc tctatcggct gcaggtcgac
    19801 aagtccctgt cgttcggcga gaaaaagatg ctcgagacgg cgcgtaatct ggtcgtgaag
    19861 gagatcgcgg ttgcgcgttc caagaccgaa gagagcacgc aggccgaaat cgagcagatc
    19921 ttcgtgaaga actgaacctt ccgaggaatc ccacggcggc cctgcgcgct cggcattcga
    19981 tgtcgcgacg gggcctccgc ttcgtgcaga atcgacctcg agacaaagcg ctcggtcgac
    20041 cgcgtccggt cctagaaaac gctgctaacg gatacacgaa tggcgaaaaa cacacttctg
    20101 gtcaacgtcg acatcggaga aacgcgcgtt ggtctcatcg cggacggtgt gctccgcgag
    20161 ctctacgtcg agcggcggca tcatcgctcg ccggtgggaa acatctacct cggtagagtt
    20221 acccgagtac ttcccgggat gcaggcggcg ttcatcgaca tcgggctcga ccgggcggcg
    20281 ttcctgcacg tggaagacct ccaggccccg cccggctcgg aggaagagcc cgagggaaac
    20341 gacaagcgcg gcccggccaa gggccggagt aaccgggtgt ctcgaaagac accgattcgc
    20401 gacctgctca atgagggaca gcagctgatg gtgcaggtct cgaaggggcc gatcgggacc
    20461 aagggcgccc gcgtcacgag ccatgtggcg cttcccgggc gctacgtcgt gttcatgccg
    20521 actgtcgagc atgttggcgt ttccaagcgg atcggaaacg atcgcgagcg gcggcggctc
    20581 aaagaggtga tcgattcggt gaagcctcca cagggcggcg tggtcgttcg caccctggcg
    20641 caagggctca cgaagaagaa gctcaaggcc gacatcggct acctcgtgaa gctatgggaa
    20701 cagacacaga cggcccgtga ggcggtgaag aagaccccgg ccctcgttca cgaagaaccg
    20761 gacctcgtga tccagtcggc acgggatctg ttcaccgagg acatcagcga aatcgtcgtc
    20821 gatgaccgcg acgagtatca gcgggtcaag gagttcctca cgctcttctt gcccgagcga
    20881 gccaacgacg tccaactcta cgaagatgac gagcccctat tcgacgaata cggcatcgaa
    20941 gaagagatca cccgtgcgct gtcgcgaaag gttcaccttc cttcgggggg gcacctagtg
    21001 atcgatcagg cagaggcgct gaccgctatc gacgtcaaca cggggcggtt cacgggcaaa
    21061 gggcgcgatg tcgagcagac gattctgcaa accaacctcg aagccgttcg cgaaatcgcc
    21121 taccagcttt tgttccggaa cattggcggc ctgatcgtgc tcgacttcat cgacatggaa
    21181 aggcaggcgc accgcgagaa ggtgttcaaa gcgcttctga acgccctgaa gaacgacaag
    21241 tcgcggacca ccgccattcg catcagtgat ctcgggctcg tggaaatgac ccgaaagcga
    21301 acacgacagt cactcgggcg aacactctac gagccttgtt tcttctgcga tggaacagga
    21361 ctgcttcagt cgaaggaaac cgtctgccac gagatcttca ggcaaatgag gcgggagcgg
    21421 gatgccctgc ctgggtacaa gatcgtcatc agcgcacatc cggcggtctg cgacatgctc
    21481 gaacgcgagg aaaaggcgtc ggtcgcggag gcggcacgcc gattccagcg gcgtatcgaa
    21541 ttgaacccgc gaaacgacta tcatctcgaa cagttcgacc tcacgggaga ctgaattctg
    21601 atgagcaacg acgacggtca acgaatccaa aagcgcgtca cgatcaacaa ggagttcgaa
    21661 tcgttcgatg ctttcgttca cgagtacgtc acgaacgtat cgaggtcagg tgtgttcatc
    21721 cgctcgaagg aaccgctcgc ggtgggcacg gaggtccacc tcacctttac cgtgatcatg
    21781 gacgatgtgg agaccatcga aggcgccgga gaggtggtcc gggtccaaga cgacccgccc
    21841 gggatgggcg tggccttcac caagctcacc aagtattccg aagatctgct cgtccgcctc
    21901 ttgaccctgc acggcgctgc gggctcatga cacggctgag cgagggcaag ttcggcccct
    21961 ccggcaagaa gcttgccgac ctcagcgacg acgaggttct cgcagaacgt gcgaaatgcc
    22021 gcggcgaacg gtctaccagc gagtcgcgac cgagttggaa acgggtgaag cagtacctgg
    22081 ccaacctgga gctcaacccc ggggcaacct ggcccgaaat cgagcgcgcc tacgagcgac
    22141 tgcgcgaacg ttacaacccc gagaaacacg aagcagaccc cgctcgcttc cgcgctgccg
    22201 aggagctcaa cgcctccctc gcgagcgcgt accaagccct ccgccgctac ttcaggtccg
    22261 agtgagccga ggcgcgctgg ctcaggtgct ggtcaggtag cctttttcca acagggccac
    22321 gaccagctcc gcgacgtccg gatcaggccg gccactgtgg ttcatcatgt cggcgagcgt
    22381 attgtggttg agcgcgagtt ggatgacgtc gagctcgtcg gggctcgcat ctcgcagagg
    22441 cttcttgagc ggaaacgcga cggacatttt cgagcggagc ggcgggagat cgtctccgag
    22501 acggcgcatc tcgtcgagct ggcgcatgcc ctccatcaga aggccttcga tactgccctg
    22561 gatctgcgta ggaaaagttc gttcgacctt tggaagcaag tcgaaggtgc ccttgtccca
    22621 gaccagcaca cgatagaacg ccttctcagg cgggaccgat tcgtctccgt tgacgactgc
    22681 aaactggacc ctgccttcat cgaggtagat cgacccgacg tcgctctccg tggtgatctg
    22741 caacacgccc gtcttcttgg atgcggagaa gagctgcagg agatcgggaa gtgcaacctc
    22801 ggcgatcgag ccagtcatgg tgctcgccgc agacgcgggg cgcgcactcg aggctgccgc
    22861 gtccatcttg gccttcgcgt cctgcaacga agcggagccg tccgaggcca cgaccttgat
    22921 gatgctggtg ccgatgagga tgcggtcccc ttcggcgagc ttggtgcggc tgatcttctc
    22981 tccgttgacg aagctaccgt tggtggaacc aaggtcctcc aagaagatgt tcccgttctc
    23041 tacggagatc ttggcgtggc ggcgcgacac catgtcctct acgagaacca tgtcgagctc
    23101 gctggaccgc ccgatgacga tctcgccatc gtcaggaagg ggaaattccc caccctggta
    23161 cttcccagag atgaaacgca gcgccaacgg caacgtcaga ccttcgatga ccaactcccc
    23221 ttgctgagac cccattgtag cgaaacccca ccttctacta gagttaaggc taggagctgg
    23281 ccatgcatgc ggtcaagccc ctggcaacga aaggcggcca tgaggcgggt atcggctctc
    23341 gagccccggg cccggcggcg ataaatggcc cacctgctgg gtcttttggc tgcattaccc
    23401 gcgcacccct cagcgggtgc tggccggctg ttccagaggc ctagacctcg agaatgtcgg
    23461 tttctttttt ggcgacgatc tcgtcgacct tggcggtggc ggttttgacg aggtcctcga
    23521 gctccttgcg agcgcggtcc gcgtcgtcct cacctacatc accggctttc tgaagactgt
    23581 cgaccatgtc tttggcttcg tgccgcgctt tgcgaatcgc gaccttgcat tcctcaccgg
    23641 acttccgggc gactttgacc aagtccttgc gccgctcctc ggtgagggga ggcatcggaa
    23701 tacggatgat ctcaccatcg ttggacgggt tgagcccgag ctgagcctcc ctgatggcct
    23761 tttcgatgac ctgcatttgg ctccggtcaa acggcttgag gacgatcatc cgaggctccg
    23821 gcaccgacat ggaggcgagc tgcttcaaag gcgtctgcga gccgtagtag tcgacgcgca
    23881 ggccatcgag aatcgcaggg ttggcgcgcc ctgcacggat tttcgcgaga tcacgcctga
    23941 gggactcatg cgccttgtcg atcgacgcgc ctaactcttc cagtgtttca tcaagcatcg
    24001 gattcctcga atcgagcggc tgtcacccct ccgcggtcac taggctaccg acgtcttcgc
    24061 ccatgacaac ccgtttaatg ttgccgggct ccgtcaactt gaaaacccgg accggcagat
    24121 tgttgtcgcg gcatagggcg accgcggtgg cgtccatcac ccctacccgc tccattagga
    24181 agcgatcgta actcatgcgc cggaccatgg tggcgtcatc gtgacggacc gggtccttgt
    24241 cgtaaatccc cttgacctta gtggccttgc agagtgcgtc cgcgcggatc tccatggcgc
    24301 ggagcgccgc cgcagtgtcc gtggagaagt acggatttcc ggttcccgcg acgaaaatga
    24361 cgacccggcc cttttccaag tggcgaaccg cctttcgtcg gatgtacggt tcgcagaccg
    24421 ccttgatttc cagcgcgctc atcacgcggg tgtcgactcc cgccttttcg agtccgtcct
    24481 gaagggcgat tccgttgatg acggtggcca acatgcccat gtggtcggcc gcagtgcggt
    24541 ccatgccgct ggcactgccc ttgaggccgc ggaagatgtt gccgccgccg atgacgatgc
    24601 cgatctgaca cccactcgaa tgaacttcgg ccagctcctc gctgatcgct ttcagggtct
    24661 cgggctcgat gctcttaccg gcgggtccgc cggaaagcac ctcacccgag agcttcagga
    24721 gtacgcgact gagcttggcc ccgttcgtca tggctcaaga gccttttagc gcttctgtca
    24781 cctctgccgc cagatcggat ttctttttct cgattccttc cccgagctcg taccgtgcaa
    24841 agcgacggat gctgatcttc tcgcccagct tcgcagtaag cgcctcgagg agctgacgaa
    24901 tcgtcttttc gtcgtgcaag atggacacct ggtcgagtag gcaggactcc ttcttccact
    24961 tggcaagctt gccttcgacg atcttttcca cggcggctgc gggcttttcc ttgccagtcg
    25021 ccttggcttc ctcttcgagc tgacctcgga agatggccat tcggcccagg agctcgttct
    25081 cgggaatgtc gtcctcgctc acgaagagcg ggttctcggc ggccacctgc atggcggtct
    25141 cctcgacgaa ctgccggaag tcgtcgccac gcgcgacgaa gtccgtctga cagttgatct
    25201 cgatgaggac cccgacccgg cccgcgccat ggatgtacgc atgcacgagg ccgtcgccag
    25261 cgatggcgcc tgctttcttg gcgaccttgc tgagaccctt cttctggatg atctcgatcg
    25321 ccttttcctc gtcgccctcg gtttcacgaa gcgcgtcgcg gcaggccatc atgcctgcgc
    25381 cggtgcgatc gcgaagtgat ttgaccatgg atgcgctaat ctcagacatc attcttttcc
    25441 ttcgggagcc gccggggctg cgggagcggc cggagcggaa gcgcgcttgc gagcaaactc
    25501 gacctgaaca ccttcggcgg atgcgcggtc gggcgcctgt gcgccatcgt gctggaggaa
    25561 atcacgtcgc gcctggctac cttcgatgca tgcatcggcg attcgggacg tgatgagctt
    25621 gatcgagcga agcgcgtcgt cgttgccagg caccacgaag tcgatcaggt ccggatcgca
    25681 gttggtgtcg gtgagcgcaa cgatcgggat atggagcttg cgggcctcgc tgatggcaat
    25741 gtgctcgttg tgcgggtcga tgacgaaaag caccgacggg agactggtca tcatcttgat
    25801 gcctccgagg tacttctcga gcttctcggc ctcgcggcga atcgaaagcg cttccttctt
    25861 gcgaagcgcg ttgatcgtac cgtcttcgac ctgctgctcg attgcacgaa gccgatcgat
    25921 gccgttcttc atcgtgcgga agttggtcag cgtgccgccg agccagcgac ccgtgacgaa
    25981 gaactgccca gcgcggtttg cctcgtcgcg aacgatttcc gccgcctggc gcttggtgcc
    26041 gacgaagagc acgtgcccac cacgcccgac gctgtcggta acgaaggcat acgcacgctt
    26101 gaacagctcc gcggtctgat cgagatcgat gatgtggatg ccattgcgtg cgccatagat
    26161 gtaggggcgc atccgcgggt tccagcgctt ggtctgatga ccaaagtgca caccggcgtc
    26221 gatgagggcc cgtaggccga tgggtagatc gcccgacgcg acgtcggggc ctgccagggc
    26281 cgctagctcg ggcgcttcct gggtctcaag agtaggttca gccattgctc atttctcctt
    26341 tcggttgaac tgccatcccc ccagacgaac aactcccgaa ggagcaccgg aacgtctgat
    26401 cgagccgggg gatgtgggtg atttcggggg ccgaggcctc cggcttcaac tgaagcggcg
    26461 aagctatacc agggggcgcc ggcggatgcc aggcgaggag gatcgggacc agtgccagtg
    26521 tcagtgaatt aatcctctaa tttcgaccaa ataggtggcg tgtcggcttc ggggtcctcg
    26581 gcacccaggc gccgggcggc ccgaaggatg gttgcggtgg tctttagggc ctcttgcagg
    26641 tgcggagccc agggcccttc ccgggagcgc gcagcaccct ggctcgccaa cgcgcacgcc
    26701 ggatcgtcga cgtccccgag cgggtcctcg ggcagcgtgt cgtagtagat ccgaaaatcg
    26761 gtggtcggag cgagtgcttg ccgaaggccg tgctcgccgg taagccaagc ttgcagtcga
    26821 agacaggcgt gttcgcgaac cagatctcgc tcgctttcgt tgtcgagacc gaaatccgga
    26881 accgcgaggc tctcctgtcc ctcgatcaac gaatgacaga gctcatgcag aatcatctgc
    26941 gccaagcaat cgtcggcatc gagcgtgctg ggatcgccga tgatcatgac gccctgcccg
    27001 tccgtcgacg catacacgtc ggcagagcgt tccactcgaa atccaattcg gccggccgtg
    27061 gtgagccaaa cggcatcgag cggatcgagg tagctcaggc gggggattcg attcacttcg
    27121 acgcaaactc tgaaatcaac cgaagcgttc gtctagcgct cgttcgtaga gagcgcgacg
    27181 gcggccgatc tcggcgggct gcttcagcgg aacggctagg cctcccagaa atggggtttt
    27241 ccgtgctacc gttcgaccta gtgggagccg catgctgtcc aatttgcaag aaacagctat
    27301 ccaaacggac caagaacttc ccgttctgtt cggagcgatg caagct
//
LOCUS       DASRZF010000094         8437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1100056, whole genome shotgun sequence.
ACCESSION   DASRZF010000094 DASRZF010000000
VERSION     DASRZF010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 8437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8437
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100056"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="VFG22_04290"
     CDS             <1..762
                     /locus_tag="VFG22_04290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HET6415490.1"
                     /translation="AVMVTRNRVALARRALLCLRNQTWPNLDLVIVDDGEESYEGLVV
                     EFEDDFPIHYHRMKPNPELKLGGLRNLSEDLAQGEYLVQWDDDEWFHPDRISYQMETL
                     REQNLDAVVLRWTLVHLDSPEFVHHSYRSGLARGVPGTILHRRTSARYPNWRRHEDAV
                     FRRRVKRRGRLGIATGDHAHLFIRCFHGTNTWDQKHFTERLHYTWRDKLRYLRARFID
                     GDVLKLPAFNLNIDEVASVQRMIRESRELGLLQHG"
     gene            762..2276
                     /locus_tag="VFG22_04295"
     CDS             762..2276
                     /locus_tag="VFG22_04295"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HET6415491.1"
                     /translation="MAYQAALLKGTLVNTLGLMTKLLYPLLIFVLTRLFGAGLMGHYF
                     IALAVVELGTGAVTSGWATATTVRVSPHAEAAGDDAEANARMNAMLGRTLSYSLATAL
                     LFGLLVQGLGSWTIHNYFTKHVELLPGIYFVGWAMVPTAFANIVGAAGKAHLKMAWDA
                     ILGGARPLFLLVTSVLVYFLGGGLTELLASYFLSMFAVALLSLIPLVRYFDFREVLRN
                     VRPVWDGELVHFAVPEGLTHTLTLYITRLDTIMLGAMGVEPVTLAWYATASYVTSNLQ
                     QLRIVFSTALAPVVARHHYRREREELTPLLTRTTRWVSSILLLFALSFVVLRRDILAF
                     VDRSYARPGDGFVLVLLIPAVVSCGFGLAGNFITYTGHSRVNLVNGIFIGALNTMLNL
                     LWIPPYGLMGAACATAVSATIVGILTLIELRVLENITIRGRDAWMPYAGAAVGAAILG
                     LFWDPADFRGLGARLLLTLAVLVAYGVGLWVSGHPEVRAWVRRQRPARHPLHRR"
     gene            2385..3398
                     /locus_tag="VFG22_04300"
     CDS             2385..3398
                     /locus_tag="VFG22_04300"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET6415492.1"
                     /translation="MGHRTFQDRLRPLPPYPLKTATRALNAMWRPESPLLAQLVVIRR
                     CNLSCGYCNEYDDYSPPLPKEELFARIDHLADLGCLILTVTGGEPFMHPDLDQIVSHA
                     VSRGMVVTAISNAYPITKGWIARMNDAGLSLLQVSVDNVEPNEVSQKSWSKIKKRLLL
                     LQEQAKFKLNVNAVLGSSPPEQARQLIDEIRSLGFYMTVGLLHDEKGQIDPGLIGDVL
                     PEFYEEMRALCNKSFFHRFGEGWEQKMLRGGTAPWRCRAGARYLYIDEFGKVAYCSQR
                     RDEPGIPLLEYTRRDLKREYDRPKGCEDGCTIACVRRASSLDEWRPQRGPVATPKQSL
                     PVI"
     gene            3495..3707
                     /locus_tag="VFG22_04305"
     CDS             3495..3707
                     /locus_tag="VFG22_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415493.1"
                     /translation="MLAKTLIRALALVAVFAFAAVGSSEASAYCPGDGDKPLEPTTFC
                     PGDGDKPSEPSTYCPGDGDKPSEPTT"
     gene            3768..3932
                     /locus_tag="VFG22_04310"
     CDS             3768..3932
                     /locus_tag="VFG22_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415494.1"
                     /translation="MPKIDASEEAKFIGVGARNEEDSGEYSDIDVRFGPGRCSGAKTG
                     PYLHKDGTPY"
     gene            4002..5504
                     /locus_tag="VFG22_04315"
     CDS             4002..5504
                     /locus_tag="VFG22_04315"
                     /inference="COORDINATES: protein motif:HMM:NF016946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HET6415495.1"
                     /translation="MRIDKSFLRDRFLGIDIRTLGLARIYIALLLLFDLTRRSLEMSI
                     WYFESGLLPNSMLAEHPLRPWAYSFLYYVSSDTGVRLAFVLIALVYLALLFGVFTRFA
                     QVLAAICLISLQVRVDFLSNGGDFVFCNLLVWTAFLPLGSAFSFDAWRKKNRNTPTES
                     PIASWAVLVVTLQLAVIYFFNAIHKNGETWSDGTAVYWLAQQERIVTWFGLWMRQNLP
                     LWVFQAMTYGSLVLEYLLPILILSPWGRPWTRRAAIAGIWGLHLGIAAVANVGSFSLV
                     MIAYSMFLVSPEDWEWLRGKLAALRGPNARAVAALALPAVDSAQTLKRSPLRWLSHAF
                     LALLVVAAISQVLVENWSVPKFLKHQQPTWIQATIQTFRLNQGWSMFASNAPRDDMWI
                     VVDATTIDGRQIDPYNELATRYADPTLRTIPPRLDMSYWWCDYTVRIPGFRQYHSSLT
                     DWIFRHHERTGNENDRIVSFRAYTVSQIPPAPGESEPREVNVKPFLAKRR"
     gene            complement(5557..6411)
                     /locus_tag="VFG22_04320"
     CDS             complement(5557..6411)
                     /locus_tag="VFG22_04320"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6415496.1"
                     /translation="MSARTQAIRGAGGHELFLDLKGRGRIRVIEYQGPEGAPTLMLLH
                     GLGATGPLNWFTAFSDLSERYHVVAVDHRGHGRGIPTRRFRLKDCADDVVAVADELGI
                     GKFIAVGYSMGGPIAKLCWSRHPGRVRGLVLCATAKHFTPERYPVLTRALLPGAVIGA
                     RMAPKLVLGQIIEGMVRDIPEPKVKDYVRKEMAGADPAALAQATRAVLRFSSRNWASN
                     IAVPTAVLVMTRDKIVPTRRQYALAAAIPNVRQFAVDGDHFACANTKTNFVPELLAAC
                     KHVDAAAA"
     gene            complement(6517..7446)
                     /locus_tag="VFG22_04325"
     CDS             complement(6517..7446)
                     /locus_tag="VFG22_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415497.1"
                     /translation="MSTRGMGNRHRKNAVPNMMLCAISTILLLSAGCRWRHGVPEGSP
                     GHVSDANQACATLFCSPEEQLETDGQCAPSCMCGRYTFSEPRYDEHDTHALRGWRLVN
                     PTEALPGDPFSEESIAREETQGYCAAIPVDRSPQVRTYRLETFPTERAALAAGGQITH
                     RGRCGACSSFQDLAVYIEQKDLNRAGRLCGLRGTLGSKGQMSCFRKLGFTEACAQIWS
                     FNVDHTRSECMGSCMATIPTKHKRPDGSLNPCLACDEENSGPVFKAVAGRTRRSSGLS
                     SAIARPCLDAQGEPAVYPVEHYYFSQRASRARN"
     gene            complement(7509..>8437)
                     /locus_tag="VFG22_04330"
     CDS             complement(7509..>8437)
                     /locus_tag="VFG22_04330"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415498.1"
                     /translation="IVVATVIAVLYTYLGGLLGDVWTDIVQGALVVLGLCTTLFLLVS
                     DLGGWSEALARIEPEQLRLIPPGESPWARANVWLVPVIGALIAQESVSRALGCRNARI
                     AKRAALLAGGFYLTLGIVPVLLGLLGAHYPLPLSANEDFLPELARHTLPPWAYVLFVG
                     AIVSVILSTIDSSLLAIGALAARNLFAERSGLHDSSRGLLTARLFVILAGALSCFVAL
                     SAESIYDLVLEADALGTAGIAVITIAALFTSAGGPVAAVSTLVVALVSSASGKYLFDY
                     EAPFLLSLVLSIAVFVLVSRFERSKEPSPGKL"
BASE COUNT         1526 a         2603 c         2614 g         1694 t
ORIGIN      
        1 gcggtcatgg tcacccgcaa tcgagttgcc ctcgctcgca gggcgctcct ttgtttgcgc
       61 aatcagacgt ggcctaacct cgatctcgtc atcgtggacg acggcgagga gtcttacgaa
      121 ggcctagtgg ttgagttcga ggatgacttc cccatccact atcaccgaat gaagcccaac
      181 cccgagctaa agctcggcgg cttgcgcaat ctctccgagg atcttgcaca gggcgagtat
      241 ctcgttcagt gggatgacga cgagtggttc catcccgatc gcatcagcta ccaaatggag
      301 acgcttcgcg agcaaaacct agacgcggtc gttctacgat ggaccctcgt gcacttggat
      361 tcgcctgagt tcgtccacca ctcctaccgc agcggtttgg cgcgcggtgt tcccggcacg
      421 attcttcacc gacgaacttc tgcgcgctac cccaattggc gacgtcacga ggacgccgtg
      481 ttccgcagac gtgtgaagcg gaggggccgg ctcgggatcg cgacgggtga ccacgcccac
      541 ctgtttattc gctgctttca cggtacgaac acctgggacc agaaacactt taccgaacgg
      601 ttgcactaca cctggcggga caagctccgc tatcttaggg cgcgtttcat cgatggagac
      661 gtgctgaagc tcccggcgtt caacctgaac atcgacgagg tcgcttccgt acagcgaatg
      721 attcgggaaa gccgcgagct cggtctgctc caacacggct gatggcgtac caggcagcgc
      781 tactcaaggg taccctcgtc aacacgctgg gtttgatgac gaagctgctc taccccctgc
      841 tgatcttcgt cttgacgagg ctgttcggcg cgggcttgat gggtcactat ttcatcgccc
      901 tcgcggtcgt cgagctcggc accggagcgg tcacctccgg ttgggccacg gctactaccg
      961 tacgggtgag cccccacgcc gaagcggccg gcgacgacgc ggaagcgaac gctagaatga
     1021 acgccatgct cgggcgcacg ctgagctata gtctggcgac ggcattgctc tttggtttgc
     1081 tcgtccaagg actggggtcg tggacgattc acaactattt caccaagcac gtcgagttgc
     1141 tccccgggat ttacttcgtg ggctgggcga tggttccgac ggcatttgcc aacatcgtcg
     1201 gcgcggcggg gaaggcccac ctgaagatgg catgggatgc gatcctcggt ggcgcgagac
     1261 ctttgtttct gctcgtcacg agcgtgctcg tgtatttctt ggggggcggt ctcaccgagc
     1321 tcttggcgag ctacttcctc tcgatgttcg ctgtagcgtt gctttcgctc attccgctcg
     1381 ttcgctactt cgactttcgc gaggttctcc gcaacgttag gcccgtttgg gatggcgagc
     1441 tcgtacactt cgcggtgccc gaggggctga cgcacacact caccctttac atcacgcgtc
     1501 ttgacacgat catgctcgga gcgatgggtg tcgagcccgt aaccctagct tggtatgcga
     1561 cggcatcgta cgtgaccagc aacctacagc agctccgcat cgtattcagt acggcactcg
     1621 ccccggtcgt ggcgcgacat cactaccgtc gcgagcgtga agagttgaca cccctgctca
     1681 cccgtacgac acgttgggta agctcgattc tgctgctctt tgcattgtcg ttcgtggtgc
     1741 tccggcgaga catcctggcg ttcgtggacc gaagctatgc gcgtcccggg gacggcttcg
     1801 tcctggtgct cttgatcccc gcggtcgtca gctgcgggtt tggccttgct ggaaacttca
     1861 tcacctacac aggacattcg cgggtgaatc ttgtgaacgg catcttcatc ggcgctctga
     1921 acacgatgct caaccttctg tggataccgc cgtacggtct gatgggggct gcgtgcgcca
     1981 ctgctgtttc agctacgatc gtggggatcc tcacgctgat cgagctacgc gtgctcgaga
     2041 acatcacaat taggggccgc gatgcgtgga tgccgtacgc cggggcggcg gtcggcgctg
     2101 cgattctcgg cctgttctgg gatcccgccg actttcgcgg cctcggcgcg cggctcctcc
     2161 tgacactcgc ggttctcgtc gcgtacggcg tcggtctttg ggtctcaggc catcccgagg
     2221 tacgtgcttg ggtccgacga caacgtccgg cacgtcatcc gctacatcgc agatagttga
     2281 cgtgcgcccc gtagagcgtt cgccggggac ttccccccgc tggaacagcg cggctctttc
     2341 tgcgcttgac ctcaaatcca ccccctgcca gcgtgtgcca atcgatgggt catcgtacct
     2401 ttcaagaccg actgcgcccc ctgccgccgt atccactcaa aacggcgaca cgcgcactca
     2461 acgccatgtg gcggcctgag tcgcccctcc ttgctcagct cgtggtcatc cgcaggtgca
     2521 acctgagctg tggctactgc aacgagtacg acgactattc accgcccctg cccaaagaag
     2581 agctcttcgc ccggatcgac cacttggctg accttggctg cttgatcctg actgtgactg
     2641 ggggtgagcc attcatgcat cccgacttgg accagattgt ctcgcatgca gtctctcggg
     2701 gcatggtggt gactgcgatt tccaacgctt acccgatcac gaaagggtgg atcgcgcgca
     2761 tgaacgacgc gggtctttcg ctcttgcagg tttccgtcga caatgtggaa cccaatgagg
     2821 tgtcgcagaa atcatggtcg aagatcaaaa agcgactcct gctgctccag gaacaggcga
     2881 agttcaagct caacgtcaac gcggtgcttg gatcgtcgcc gccggagcag gcccgccaac
     2941 tgatcgatga gattcgctca ttgggtttct acatgaccgt tgggcttctg cacgacgaaa
     3001 aggggcagat cgatccgggg ctgatcggcg atgttcttcc cgagttctat gaggagatgc
     3061 gagcgctctg caataagagc ttctttcatc ggttcgggga ggggtgggag cagaagatgc
     3121 tccggggcgg cacggcgcct tggcgttgcc gagccggcgc ccgctatctc tacatcgacg
     3181 agttcgggaa ggtggcctat tgcagccagc gccgagacga acccggcatc cccctactcg
     3241 aatacactcg acgcgatctc aagcgcgaat atgaccgccc gaaaggctgc gaggatggct
     3301 gtaccatcgc gtgcgttcgg cgcgcctcca gcctagacga gtggcgcccc cagcgaggcc
     3361 cggtggcgac cccaaaacaa tcgctcccgg tgatctaggc cctcactcgg agtcgtctcg
     3421 agcggatctc atcggccgaa ccaggcgtac gaatggggct tgtgcacctc gtgggagggc
     3481 tctaacttct cctcatgctg gcaaagacat tgattcgcgc cctggctctc gtcgccgtct
     3541 tcgcattcgc ggcggtcgga agttcagagg catctgcata ctgtccgggt gacggcgaca
     3601 aacccttgga gccaacgacc ttctgcccgg gtgatgggga caagccctca gagccgagca
     3661 cctactgccc gggtgatggc gacaagccct ccgagccgac gacctaaaga agaaggcccg
     3721 ctgcggtacc cgttgttcta gggaggccga tccgttacgg attgtccatg cccaagatcg
     3781 acgcgtccga agaagccaag tttatcggcg tcggagctcg caacgaagaa gactccggcg
     3841 agtattcgga tatcgacgtg agattcggcc cgggccgctg ctcgggcgcg aagaccggtc
     3901 catacctgca caaggacgga acgccctact gattgctctg gggggtgtgt tgccttggtc
     3961 gacgaggcga ggctgctacc gtcggcccac agagctcgcc catgcgtatc gacaaaagct
     4021 ttctccgaga ccggttcctc ggcatcgaca ttcgcacgct cggcttggcg cggatctaca
     4081 tcgccttgct gctcctcttc gacctcacgc ggcggagcct ggagatgtcg atttggtatt
     4141 tcgagtctgg gcttcttccg aactcgatgc tggcagagca tccgctccgg ccctgggcct
     4201 actcgttctt gtactacgtc agctcggaca cgggggtccg gctggctttc gtcttgatcg
     4261 cgctcgtcta tctggcgttg ctgttcggtg tatttacgcg cttcgcgcag gtactggctg
     4321 cgatttgcct gatctcgctg caggtccgcg tcgatttcct gtcgaacggt ggcgacttcg
     4381 tgttttgcaa tctgctcgta tggacggcat ttctgccgct tggctccgcg ttctcgttcg
     4441 acgcctggcg aaagaagaat cggaacacgc cgaccgaatc accgatcgcg tcctgggccg
     4501 ttctcgtcgt gaccctccag ttggcggtca tctatttctt caacgccatt cacaaaaacg
     4561 gcgagacttg gagcgacggg accgcggtgt actggctggc ccagcaagag cgaatcgtga
     4621 cctggtttgg cctttggatg cgccagaacc tcccgctctg ggtgtttcag gcgatgactt
     4681 acgggagtct ggtcctcgag tacctgcttc ccatcctgat cctttccccc tggggacgac
     4741 cctggacccg gcgcgcggcg attgccggca tttggggcct gcatctcggt atcgccgcgg
     4801 tggcaaatgt cgggtcgttc tcattagtga tgatcgcgta ctcgatgttc ctcgtgagcc
     4861 cggaggactg ggaatggctt cgcggcaagt tggcggcgct ccgcggtcca aacgctcgcg
     4921 ccgtcgccgc gcttgcgttg ccagcagtcg attccgccca gacactcaaa cgctccccgc
     4981 ttcgctggct gtcccatgcc tttctggcgt tactcgtcgt tgcggcgatt tcgcaggtgc
     5041 tcgtcgaaaa ctggtcggtg cccaagtttc tgaagcacca acagcccaca tggattcagg
     5101 cgaccatcca gacctttcgg ctcaatcagg gctggagcat gttcgcgtcc aatgcacccc
     5161 gtgacgacat gtggatcgtc gtcgatgcga ccacgatcga tggcagacag atcgacccct
     5221 acaacgagct tgccactcgc tacgcagatc ccacgctccg aacgattccg ccgcgactcg
     5281 acatgagcta ctggtggtgc gactacacgg tgcgcatacc gggtttccga caataccatt
     5341 cgagcctcac tgactggatc tttcgccacc atgagcgcac gggaaacgag aacgaccgca
     5401 tcgtgtcttt tcgtgcctac accgtgagcc aaattcctcc ggcccctggg gaatccgagc
     5461 cgcgcgaggt gaacgtgaag ccgttcttgg caaagcgacg atgatcggtc ggagcagctc
     5521 cgcgctcgaa acgaataggc cttgagagaa gagcttctaa gcggctgcgg cgtcgacgtg
     5581 cttgcaggcg gcgagcagct caggcacgaa attggtcttg gtgtttgcgc aggcgaagtg
     5641 gtcgccgtcg accgcgaact gccggacgtt ggggattgcg gcggcgagcg cgtactgtcg
     5701 gcgggtgggc acgatcttgt ctcgcgtcat gaccaggact gcggtgggga ctgcgatatt
     5761 cgaggcccag ttgcgagacg agaaccggag gaccgcgcgt gtggcttgcg cgagcgcggc
     5821 ggggtcggcg cctgccatct ctttgcgaac atagtctttg accttcggct cggggatgtc
     5881 tctgaccatg ccttcgatga tctggccgag cacgagcttg ggcgccatac gtgcgccgat
     5941 gaccgcaccc ggcagtaatg cccttgtgag aaccgggtat cgttccggcg tgaagtgctt
     6001 ggcggttgcg cagagcacca acccacgaac gcgcccggga tgccggctcc agcagagctt
     6061 cgcgatggga ccgcccatcg aatatccgac cgcgatgaac ttgccgatgc cgagctcgtc
     6121 cgccacggcg acgacgtcgt ctgcgcaatc cttcaagcga aaccgacgcg tggggatccc
     6181 ccggccgtgc cctcggtggt cgacggccac gacgtggtag cgttccgaga ggtcggaaaa
     6241 cgcagtgaac caattcagcg gcccggtcgc gccaagtccg tggaggagca tcagcgtagg
     6301 cgccccctcg ggcccctggt actcgatgac ccgtatgcga ccccggcctt tcaagtccaa
     6361 gaagagctcg tgaccaccgg cgccgcgtat cgcttgcgtt cgagcgctca tcggtttgtt
     6421 taccaacagt ctgaagcctg ccggcggctg aggccgtgcg caaggcggtg ggaggctggt
     6481 tgccctatct cgtccacaga ggacgagatt cgggtctcag ttgcgggccc gggaagcgcg
     6541 ttgactgaag tagtagtgct cgaccgggta taccgcgggc tcaccttgcg catccaggca
     6601 gggacgggcg atcgccgaag acaatcccga gctccggcgg gttcggccgg cgaccgcctt
     6661 gaaaactggg ccgctatttt cttcatcaca cgcgaggcac gggttcagac tcccgtcggg
     6721 tcgcttgtgc ttcgtgggga tagtggccat gcagcttccc atgcactcgg agcgcgtgtg
     6781 gtcgacgttg aagctccaga tttgtgcaca cgcttcggtg aagccgagct ttctgaagca
     6841 gctcatttgc ccctttgaac ccaaggtccc ccgcaggccg cataggcgtc cggcgcggtt
     6901 caggtccttt tgttcgatgt agaccgcgag gtcctggaac gaagagcagg caccgcatct
     6961 gccgcgatgt gtgatttgac caccggccgc gagggccgcg cgctccgtcg ggaatgtctc
     7021 gagccggtac gtgcgcactt gggggctcct gtcgactgga attgccgcgc agtatccctg
     7081 ggtctcttcg cgcgcgatcg actcctcgct gaaaggatcg cccggcagcg cctcggtcgg
     7141 gttcaccagc cgccagccgc gcagcgcatg ggtgtcgtgt tcgtcatacc tgggctcgga
     7201 aaacgtgtag cgtccgcaca tgcacgaggg cgcacactga ccatcggtct ccagttgttc
     7261 ttcaggggag caaaaaagcg tcgcgcacgc ttgattggca tcgctcacgt gaccaggcga
     7321 gccttcgggc actccgtgcc gccatcggca gcctgccgag agcagcagga tcgtggagat
     7381 cgcgcacagc atcatgtttg gaaccgcatt cttacggtgt cggtttccca tgcctcgcgt
     7441 agacatagat gatccttcga tcgctcctgc cacacaccta attacctcga acatcgcagc
     7501 tgtcgagact agagcttgcc gggcgagggc tccttgctgc gctcgaaccg cgacaccagg
     7561 acgaagacgg cgatgctcag caccagcgaa agcaggaaag gggcctcgta atcgaagagg
     7621 tacttgccac tcgctgaaga cacgagggcc actacgagcg tcgagaccgc ggcgaccggc
     7681 ccgcctgcgc tcgtgaaaag cgcggcgatg gtgatgactg caatgccggc ggtcccgagc
     7741 gcatccgctt cgaggacgag gtcgtagatg ctctcggcgg agagcgccac gaagcaactc
     7801 agggctcccg caaggatgac gaagagccgc gcggtaagaa gccctcggga cgagtcgtgt
     7861 agccccgagc gttcggcgaa gagattgcgc gcggcgagag cgccaatcgc aagcaacgag
     7921 gagtcgatgg tggaaaggat gacggacacg atggcgccta cgaagagcac gtacgcccag
     7981 gggggcaaag tgtggcgtgc gagctcgggc aggaaatcct cgttggcgct cagcggaaga
     8041 ggatagtgag ctccgagaag gccgagcagc accgggacga tgccgagggt taggtagaaa
     8101 ccaccggcca agagggctgc ccgttttgcg attcgcgcgt ttctgcagcc gagggcgcgc
     8161 gagaccgact cctgcgcaat gagagcgccg atcacgggaa ccagccagac gttcgcacgc
     8221 gcccaagggg attcgcccgg cgggatcaag cgcaactgct ccggctcgat gcgcgcgagc
     8281 gcctcgctcc agccgccgag gtcgctgacc agcaggaaga gagtggtgca caagccgagc
     8341 acgaccagtg cgccttgcac gatgtcggtc cacacgtctc caagaagccc gccgaggtat
     8401 gtgtagagca cggcgatgac cgtcgcgacg acgatag
//
LOCUS       DASRZF010000095         2085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1100272, whole genome shotgun sequence.
ACCESSION   DASRZF010000095 DASRZF010000000
VERSION     DASRZF010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100272"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..753)
                     /locus_tag="VFG22_04335"
     CDS             complement(91..753)
                     /locus_tag="VFG22_04335"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET6415499.1"
                     /translation="MRIWVTAFALLAVVGIAALSWSMMSVPSPSEADRIAAALAPTGP
                     SAAPAAYAPCASCHLHDGSGRPDGSIPRLSGQRQSVLENKLHRLRSGMMRLPVMDPFA
                     RTLAPEDITQIARYLSQLPELAVPSNEVSPAEHAQGASLYAQHCASCHGAAGEGQDGI
                     FASRLCGQYAGYLNRRLAEAAAKSRGDADAIMQGVVDLVPSHQLGPIVGWLAEGRGCQ
                     AP"
     gene            822..1736
                     /locus_tag="VFG22_04340"
     CDS             822..1736
                     /locus_tag="VFG22_04340"
                     /inference="COORDINATES: protein motif:HMM:NF024981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HET6415500.1"
                     /translation="MEAVDQVHLNFNPATLTVLNILIGLIMFGVALDIRVDDFKRIVR
                     DPRGPLVGLGAQFLLLPAMTFLLVLIVQPVPSIALGMFMVAACPGGNFSNFLAHHAKA
                     NAALSVTMTAISTALAIVMTPANLAFWGSLNPDTAAILKEVNLDPFDLFKTILIILGV
                     PLLAGMLVAAKKPRFAERARQPMKVFSLSAFGLFIVGALIGNWQHFLENVGYVAFIVA
                     LHNAVGLALGYWSGRFLGCHRYDARAISIEVGIQNSALALVLIFGFFDGLGGMAIIAA
                     WWGVWHLISGLTVATYWSKHPVPIPASS"
     gene            complement(1746..>2085)
                     /locus_tag="VFG22_04345"
     CDS             complement(1746..>2085)
                     /locus_tag="VFG22_04345"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF044671.1"
                     /note="catalyzes a two-step reaction, first charging a
                     glutamine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; in Deinococcus the extra
                     C-terminal extension of YqeY domain enhances affinity for
                     tRNA-Gln; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HET6415501.1"
                     /translation="PDGRKVRGTIHWVSAKHAVDAEVRLYDRLFNEENPLDEAHAGDF
                     TARINPDSLEVLKDAKLEPNLGSRGAGERVQFERLGYFVVDPDSTPERPVYNRTIQLK
                     DSWSKLIKKN"
BASE COUNT          353 a          664 c          648 g          420 t
ORIGIN      
        1 tggccgcagc cgcgagactg ggcgcgccca gggatgctgc tgctgcgcct ccggtgaggc
       61 ggacgaatcg tctgcgtgta aatcgatcgc tcatggagcc tggcatcccc ttccctcagc
      121 caaccatccc acgatgggtc cgagctgatg gctcggcacg agatcgacca cgccctgcat
      181 gatcgcatcc gcatcgcctc ggctcttagc ggctgcctcg gcgaggcggc ggttcagata
      241 gccggcgtat tgaccgcaaa gcctcgacgc gaagatcccg tcttgcccct ctccagccgc
      301 accgtggcaa gatgcgcagt gctgcgcata gagcgaagcg ccttgggcgt gttcggcggg
      361 gctcacttcg ttcgagggga ccgcgagctc gggcagctga gaaaggtacc tcgcgatttg
      421 ggtgatatct tcgggcgcca atgtccgagc gaacgggtcc atgacgggga ggcgcatcat
      481 ccccgatcgg agacgatgga gcttgttctc caagacggac tgccgctgtc cgctcaggcg
      541 cggtatcgaa ccatcgggcc tgccgcttcc gtcgtgaagg tggcaagaag cgcagggcgc
      601 atatgccgca ggcgccgcgg acggccccgt cggcgccaga gctgcggcga ttcgatcggc
      661 ttccgagggg ctcggaacgg acatcatcga ccaactcagc gccgcgatac cgacgactgc
      721 gagcagcgcg aaagccgtaa cccaaatacg caccgtgccc ggagcatacc agaggaatcc
      781 gcgcaaatgc ggtcgacgat gtggtactga ggtcccgctc catggaagcc gtcgatcagg
      841 ttcacctgaa cttcaacccc gccacgctca cggtgctgaa catcctgatc ggcctgatca
      901 tgtttggcgt cgcactcgac atccgcgtgg acgacttcaa gcgcatcgtt cgcgatccgc
      961 gcggtccact ggtgggtctt ggagcccagt ttctgctcct tcccgccatg acgttcctcc
     1021 tcgtactcat cgtccagcca gttccctcga tcgccctcgg catgttcatg gtagccgcgt
     1081 gccctggggg aaacttctcg aacttcctgg cacaccatgc caaagcgaat gcggcgctga
     1141 gcgtgaccat gacggcaata tcgacggcgc tcgcgatcgt gatgacgccg gcgaacctgg
     1201 cgttctgggg cagtctgaac ccggatacgg ccgcgatcct caaggaggtg aatctcgacc
     1261 cgttcgacct cttcaagacg atcctcatca ttctcggcgt tccgctcctc gcgggcatgc
     1321 tagtcgccgc caagaagccg cgcttcgccg aacgcgcccg ccagcccatg aaggtctttt
     1381 cgctcagcgc cttcgggctg ttcatcgtcg gtgcgctgat cggcaactgg cagcacttcc
     1441 tcgagaacgt cggctatgtc gcgttcatcg tggcgctgca caacgcggtg gggctcgcgc
     1501 tgggctattg gagcggacgg tttctcggct gtcatcgcta tgacgcgcgg gcgatttcga
     1561 tcgaagtcgg aattcagaac tcggcgctgg ccttggttct gatcttcggc ttcttcgacg
     1621 gcctcggagg catggcgatc atcgccgcct ggtggggtgt ctggcacttg atctcgggcc
     1681 tcacggttgc gacctactgg tccaagcacc cggttccgat acccgcgtcg agctgagtcg
     1741 cgtggctagt tcttcttgat caatttggac caggagtctt tgagctgaat ggttcggttg
     1801 tagacgggcc gctccggagt gctgtcggga tcgaccacga agtaacccaa tcgctcgaac
     1861 tgaacgcgtt cgcctgcgcc gcgactccca aggtttggct ccagcttggc gtctttcaat
     1921 acctcgaggg agtccgggtt gatccgggcg gtgaaatcgc ctgcatgcgc ctcgtcgagt
     1981 gggttctctt cgttgaaaag gcggtcgtag aggcgcacct ccgcgtcgac ggcgtgcttg
     2041 gcactcaccc agtggatcgt gcctcgaact ttgcgaccat cggga
//
LOCUS       DASRZF010000096         5535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3509892, whole genome shotgun sequence.
ACCESSION   DASRZF010000096 DASRZF010000000
VERSION     DASRZF010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 5535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5535
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3509892"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1426
                     /locus_tag="VFG22_04350"
     CDS             <1..1426
                     /locus_tag="VFG22_04350"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET6415502.1"
                     /translation="GTAAKDFLGPVGGMVIVAGALFSMVSASNASILAGSRVALSMSR
                     LGHLPKEFGSINAQTRTPVIALMLVGAGIATFATLLPLEELAHFADCVLLIALILVNA
                     ALIQHRRKFPKLKRPFRVPLVPLLPALGILANLYLLSQVPNLATVLLALGALVVGFVG
                     FMGWKGAQVEAESLPGQRSRVALEQTDAADADFRILVPLAHPANVDALIDIASAIARR
                     HNGHIIAVRVVVVPEQLPPQLESYYVERERPILDAARRRARAHGVPVTLLVRIGHNPA
                     RAILETSRERECGLIVVGWKGHTSTARYILGEITDTIVMNARTDIMVVKLTGQALPKR
                     LLLPTHGGVHAKQAENYAADIAASQDGTVTLCSIVPLDAQEELVQSETERLAEARQRI
                     TEATKFEQVDTVVVQNRSVSEGIVQELEKYDGVIVGAAGKSFSNRILFGNIPEEVARL
                     SDKTVIVVKRHHTVKALFGRVMSE"
     gene            1504..2949
                     /locus_tag="VFG22_04355"
     CDS             1504..2949
                     /locus_tag="VFG22_04355"
                     /inference="COORDINATES: protein motif:HMM:NF025761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HET6415503.1"
                     /translation="MTDWTNYEASRFYDELVGPGGNPRAGAATLAAHLEGLSKRQLKL
                     RQEAAERAIVEMGVTFTVYTEGQNIDRAWPFDIIPRIIAASEWRRIEQGLRQRLTALN
                     MFIDDLYYEQRILEDGLVPRDLVESSPDFRRECIGARPLHGVWAHVCGSDLVRDGDGT
                     VYVLEDNLRVPSGVSYMLENRSVTKRVFPELFRDQIIAPVDDYPSDLFDTLAALSPRP
                     GDDPEVVVLTPGIFNSAYFEHSYLAQRMGAQLVEGGDLVVESDDCVYMRTVAGLTRVD
                     TVYRRINDAFLDPDAFRKDSMLGVPGLIRAWRKGNVALANAPGAGVADDKLVYTYVPD
                     MIRYYLDQDPIIPNVPTWRCSDPGHLDHVLKHLAELVVKPANESGGYGMLVGPHASAG
                     EVEKFAELIKANPRNYIAQPTLALSTVPTVCAGLIEPRHVDLRPFILQARQTAVTAGG
                     LTRVALRKGSLVVNSSQGGGSKDTWIVGGPS"
     gene            2951..3916
                     /locus_tag="VFG22_04360"
     CDS             2951..3916
                     /locus_tag="VFG22_04360"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HET6415504.1"
                     /translation="MLSRVAERMYWAGRYLERAENTARLVNTYASLLLDLPLETGIGW
                     PQLLRIVAAEDEFHDRYAKASEQNILKFLMADVENPSSIASSLAMARENIRTSRDVVP
                     SEGWECINELYLMGRDSLPGAVGRRRRFEVLSDCVMRCQQISGLLSGTMSHGDAYNFF
                     RMGTHVERADMTSRVVDVAAATIAQGNPNLAPYENALWMSVLRSLSAYQMYRQYVRRR
                     IAAGDVEQFLLADPQFPRAVAHSLDALDQCLATLPRSEEARLVVQELTKEVSRHRQSP
                     MNGAELHEYIDRLQCGFGEIHAAIRVTWFLKDPSRDSSMLPDPQS"
     gene            3913..5127
                     /locus_tag="VFG22_04365"
     CDS             3913..5127
                     /locus_tag="VFG22_04365"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HET6415505.1"
                     /translation="MTVSVDDIRRARKRIASGITSTPFLRSETLSGVTGVELYLKFEN
                     QQFTASFKERGALNRLLDFTPAQRRRGAIAMSAGNHAQALAYHGGRLGISTTIVMPRS
                     TPNAKVEQTRAHGAEVILHGNQFDETRGFTLALARERDLTLVHPYDDPAVIAGQGTLG
                     LEMLEDAPHSLDVLLVPIGGGGLISGIAVATKALAPGVQIIGVQSERYPFAHHAFHGL
                     TAPQIPNRGTIAEGIAVKSPGLLTMELIRRHVDDIVLVGEQDIEQAIFMLAEIEKTVV
                     EGAGAAGVAAVLKHRERFEGQHVGTILCGGNIDMMMLSSVLQRGMARSHRLVRLRVEV
                     PDVPGALGQVTQLIGELDSNILELSHQRAFGASSARAASVDFVLQLRGEEQAEQVLDA
                     LSARGFEASLVR"
     gene            5317..>5535
                     /locus_tag="VFG22_04370"
     CDS             5317..>5535
                     /locus_tag="VFG22_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415506.1"
                     /translation="MLVAGLQQASTPVLDDLVANGAVTYDAFAGGEPGTPTEQATFSG
                     PGWSSILTGVWVDKHGVTDNSFDGARFDE"
BASE COUNT         1050 a         1718 c         1715 g         1052 t
ORIGIN      
        1 gggaacggcg gccaaggact tcctcggccc ggttgggggc atggtgatcg tggctggcgc
       61 gctcttttcg atggtgtcgg cgtcgaacgc ctccatcctg gcgggttcgc gcgtcgcgct
      121 ctcgatgagc cggctggggc atttgccgaa ggagttcggc tcgatcaacg cgcagacgcg
      181 cacgcccgtc atcgcgctca tgctcgtcgg agcggggatt gcaaccttcg ccaccttgct
      241 tccactggag gagctcgctc atttcgccga ttgcgtgctc ctcatcgcac tcatcctggt
      301 gaatgcggcg ctgattcagc accgccgcaa attcccgaag ctcaagcggc cgttccgagt
      361 ccccttggtg ccattgcttc ccgcgcttgg gattctcgcg aacctctatc tcttgtcgca
      421 ggttccgaat ttggcgacgg ttctcttggc gcttggcgca ctggtcgtag ggttcgtcgg
      481 cttcatggga tggaaggggg cgcaggtcga agccgaatct ttgcccggac agcgatctcg
      541 agttgcgctt gaacaaaccg acgccgccga tgcggacttc cgcatattgg ttccactcgc
      601 acatcccgcg aatgtcgatg cgctgatcga cattgcgtcg gcgattgccc gtaggcacaa
      661 cgggcacatc atcgcggttc gcgtcgtggt cgttcccgaa cagctgccgc ctcaactcga
      721 gagctactac gtggagcgag agcgtccgat tctggacgca gcacgtcggc gcgcccgtgc
      781 tcatggggtt cccgtcaccc ttctggtccg gatcggccac aacccagcgc gtgcgattct
      841 ggagaccagt cgtgagcggg aatgtggtct catcgtcgtc ggctggaaag gacacacctc
      901 gacggcgcgc tacattctcg gggaaatcac cgacaccatc gtcatgaacg cccggacgga
      961 catcatggtg gtgaagctca ccggccaagc cctgccaaaa cggttgttat tgcccacaca
     1021 tggaggggtt cacgccaagc aggcggagaa ctacgccgcc gatatcgccg cctcccagga
     1081 cggtaccgtg acgctctgct cgatcgtacc gttggacgcg caggaggagc tcgttcaatc
     1141 cgaaaccgaa cggctcgcag aagcgcggca gaggatcacc gaagccacga agttcgagca
     1201 agtcgacacc gtcgtcgtcc agaaccgctc ggtgagcgaa ggcatcgtcc aagagctcga
     1261 gaagtacgac ggcgtcatcg tcggcgcagc aggcaagagc ttctccaatc ggattctgtt
     1321 cgggaacatt ccggaagaag ttgcgcgcct ctcggacaag acggtgatcg tcgtgaagcg
     1381 ccaccacacg gtcaaggcgc tctttggccg tgtaatgagc gagtagagtc gaccccgagc
     1441 atggcgcgca ctcgagaaag ccgctaaggt actgcgcttg cctaaagaag tcgatcaaag
     1501 ctgatgacgg actggacaaa ctacgaggcc agccgtttct acgacgagct cgtcggaccc
     1561 ggcgggaatc cccgcgcagg agctgccacg ctcgccgcgc atttggaagg gctttcgaaa
     1621 cgccagttga agctccgaca agaagcagcg gaacgggcga tcgtcgagat gggcgtgacc
     1681 ttcacggtct atacggaagg tcagaacatc gatcgcgctt ggccgttcga catcattccg
     1741 cggatcatcg ccgcgagcga atggcgtagg atcgagcagg gtcttcgcca gcgcctcacc
     1801 gcgctcaaca tgttcatcga cgacttgtat tacgagcagc gaatcctcga ggacggcctc
     1861 gtccctcgcg atctcgtcga atcgtcgccc gatttccgcc gggagtgcat aggggcccgt
     1921 cctctgcacg gcgtttgggc acatgtctgt gggtccgact tggttcgcga tggagacggc
     1981 acggtgtacg tgctggagga caatctgcgc gtgccatctg gcgtttcgta catgctcgag
     2041 aaccgcagcg tcacgaaacg ggtctttcca gagctgttcc gcgaccagat catcgcaccc
     2101 gtcgatgact acccctctga cttgttcgac acgctggcgg cattgtcccc ccggcccggt
     2161 gacgatcccg aagtcgtcgt tctgacccct ggtatcttca actccgcgta cttcgagcac
     2221 tcgtatctgg cccagcgcat gggggcgcag ctggttgagg gcggtgacct cgtggtcgaa
     2281 tccgacgact gcgtttacat gcggacggtg gcgggcctga cccgcgtcga tacggtctac
     2341 cgtcggatca acgacgcatt tctggatcca gacgcatttc gtaaggattc gatgctcggc
     2401 gtacctggcc tgattcgcgc ctggcgcaaa gggaacgtag cattggccaa cgcgcccggc
     2461 gcaggagtcg cggacgacaa gctcgtatat acttatgtgc cggacatgat ccggtactac
     2521 ctcgaccagg accccatcat ccccaacgta ccgacctggc gctgctccga tcccggccat
     2581 ctggatcacg tgctgaagca tctggccgag ctcgtcgtca agccggccaa tgaatcgggc
     2641 gggtacggca tgttggttgg gccgcacgca tccgccggag aagtcgaaaa gttcgccgag
     2701 ctcatcaagg cgaatccacg caactacatc gcgcagccca cgctcgccct gtcaaccgtc
     2761 cccaccgtct gcgccggtct catcgagccg cggcacgtgg acttgcgacc cttcatcttg
     2821 caagcccgac aaaccgcagt cacagcgggc ggtcttactc gggtcgcctt gcgaaaggga
     2881 tctctggtcg tgaattcatc ccagggcggc ggcagcaagg acacctggat agtaggcgga
     2941 ccgagctgac atgctgtccc gagttgcaga gcgtatgtac tgggccgggc gctatctgga
     3001 gcgcgcggag aacaccgcgc ggctggtcaa tacctatgcc agcttgctcc tcgaccttcc
     3061 cctggagaca ggcatcggct ggccgcagct cttgcgcatc gtggcggccg aggacgagtt
     3121 tcacgatcgg tacgccaaag caagcgaaca aaatattctc aaattcttga tggcagacgt
     3181 cgagaacccg agctcgatcg cctcctcctt ggcgatggcc cgagagaaca tccgaacttc
     3241 tcgcgatgtg gttccgagcg aaggttggga gtgcatcaac gagctctatt tgatggggcg
     3301 tgacagtctg ccgggtgcag tcggtcgccg ccgccgcttc gaggttctct ccgactgtgt
     3361 catgcgttgc cagcagatct cggggctcct ctcgggcacc atgagtcacg gtgacgcata
     3421 caacttcttt cgcatgggaa cccatgtcga gcgtgcagat atgacttctc gcgtggtcga
     3481 cgtggctgct gcaacgatcg ctcaaggaaa cccgaatctc gctccgtacg agaacgcgct
     3541 ctggatgtcg gttctgaggt cactcagcgc ctaccagatg tatcggcaat acgttcggcg
     3601 ccgcatcgcc gccggggacg tcgagcaatt cttgctggcg gatccacagt tcccgcgcgc
     3661 cgtcgcgcat tcgctcgacg ccctggacca gtgtctggcc acattgccac ggagcgagga
     3721 ggcgcgcctc gtcgtccaag agctgacgaa agaggtttct cgccaccggc aatccccgat
     3781 gaatggcgcc gagctccacg agtacatcga tcgccttcag tgcggcttcg gcgaaataca
     3841 tgccgcgatc cgagtgactt ggttcctgaa agaccccagt cgggattcga gcatgctacc
     3901 cgatccccaa tcgtgaccgt ttccgtcgac gatattcgcc gcgcccgcaa acgcatcgct
     3961 tcggggatta cgtcaacgcc gtttctccgt tcggagacgc tctctggagt caccggcgtg
     4021 gagctgtatc tcaaattcga gaatcaacag ttcaccgcct cgttcaaaga acgcggcgca
     4081 ctcaaccggc ttctcgactt cacgcctgcg cagcgacggc gaggcgcgat cgcgatgtcc
     4141 gcggggaatc acgcccaggc gctcgcatat catggagggc gtctcgggat ctcgacgact
     4201 atcgtcatgc cgcgttcgac acccaacgcc aaagtcgagc agacccgtgc gcatggcgcc
     4261 gaggtgattt tgcacgggaa tcagttcgac gagacgcgtg gtttcacgct tgcgttggct
     4321 cgcgagcggg acctgacgct ggtccatccc tacgacgatc cggccgtgat cgcaggccaa
     4381 ggcaccctgg ggctcgagat gctcgaggac gctccgcatt ccctcgacgt gctgctcgtt
     4441 cccatcggag gcggaggcct catctcgggt atcgccgtcg ctacgaaggc gctggcaccg
     4501 ggcgtgcaga tcatcggcgt gcagagcgag cgctatccgt tcgcacacca tgcatttcac
     4561 ggtctgaccg ctccgcagat accgaaccga ggcaccatcg cggaggggat cgccgtgaag
     4621 tcaccgggtt tgctcacgat ggagctaatc cgtcgccacg tcgacgacat cgtcctggtt
     4681 ggtgagcaag acatcgagca agcgatcttc atgcttgccg agatcgaaaa gaccgtcgtc
     4741 gagggcgcgg gcgcggccgg tgttgcggcg gttttgaaac acagagaacg attcgagggg
     4801 cagcacgtcg gcaccattct ctgtggcggc aacatcgata tgatgatgct ttcctcggtg
     4861 ctgcaacggg ggatggcgcg cagtcaccga ctggtgaggc ttcgggtcga agtcccagac
     4921 gtgcctggtg cactcggcca agtgacgcag ctcatcgggg agctcgacag caacatcctc
     4981 gagctcagcc accagagggc cttcggcgct tcttcggcgc gggcagcgag cgtcgatttc
     5041 gtgctgcagc ttcgtgggga agaacaggcc gagcaggtcc tggatgcact gtccgcccga
     5101 gggttcgaag ccagcctcgt gcgctgagcg cggcttgcgt cgatccgggc tttgagcagc
     5161 acgaacgact tctcgacgag gtagtggaga agcagcgccg ccggaagcgc cagcgagaga
     5221 tagaccagga agaacgcgag gaacacggac tctcgcggtg cggtcgcgct ggcgtcatgc
     5281 gggagcgatg gcagcgcgcc aaaatcgaaa cacgtcttgc tcgtcgcagg cttgcagcaa
     5341 gcgtccaccc cggtgctgga tgacctggtc gccaatggcg cggtgacgta cgatgccttc
     5401 gctggtggcg aaccgggcac cccgaccgag caagcgacgt tcagcggccc cggttggagc
     5461 agcatcctca ctggggtctg ggtcgacaag catggagtaa ccgacaacag cttcgatggt
     5521 gcgcgattcg acgag
//
LOCUS       DASRZF010000097        13427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1101818, whole genome shotgun sequence.
ACCESSION   DASRZF010000097 DASRZF010000000
VERSION     DASRZF010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 13427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13427
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101818"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..2048
                     /locus_tag="VFG22_04375"
     CDS             108..2048
                     /locus_tag="VFG22_04375"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6415507.1"
                     /translation="MTRPARRKWLTIPTRIVLGYILVLVAFAVVGSLSFVQHHRTSQT
                     LGLLEDGYLPLALTLSQAKATQSVYANLVERALQSERSAPTKSWLRAALRIRPTTLQR
                     AYDGVEQAETLAEELGQPAPLRSVRRELDAIATAYTEIDAKYEELFNQVDGDEAAAEA
                     LLTKLRSTEQRIERNYDRASQSVQQRISRTSALAAAQSERATFLLGLLGLLALLVGIA
                     VTFLSQRLLRPIPLLHQRVAAVAHGDLSSAVDIKRDDELGQLVAEFERMVQALAARDQ
                     SLREAALAEQRLQRMQVQIVEALRAAILVIDRDRIIQTTNPAADVMLGVSAREVGASI
                     DETSVLRRFPALGRAIAEVAKGGDAVTLEGERISGFTEHLVDVLVTPFGDELGEGGSR
                     GVLVVADDVSDAVATKLRLIQTERLAAIGRMAAHVTHEVRNPLSSIGLNVDMLGDEIH
                     GAGPESKHLLDSIQRELERLESITEEYLRLARLPEPSLTPEDPTHLLRDMAEFVRLEM
                     DTAGVELRLDLAAQLPPVAMDEPQLRQALLNLLRNAREAMPDGGTAKLEAMRYDGGVR
                     IQVHDEGTGIAEDEREHVFDLFYTTKERGTGLGLPLTQQIVVAHGGSIACKARHPHGT
                     TFEVWLPAAAQTTTGSPVRRVG"
     gene            2045..2794
                     /gene="rph"
                     /locus_tag="VFG22_04380"
     CDS             2045..2794
                     /gene="rph"
                     /locus_tag="VFG22_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HET6415508.1"
                     /translation="MTRVDGRAYDALRPVSIDANVQRNPEGSVLYRCGGTTVLIAASV
                     SDRVPDWMRDTGRGWLTAEYAMHPRANPERQRRDSRKGAIDGRSSEIQRLIGRALRAA
                     VDFEKLGERMITVDCDVLDADGGTRTASITGGMVALAMALDRLRATGLVDRGVLHAPI
                     AAISVGLLEARPMLDLCYTEDRDAEVDLNVVGTPAGGVVEVQGTAEGAPILREKLDQM
                     IDLALFAMPALAEEQRRALRAVDVDIERLLP"
     gene            2794..3396
                     /gene="rdgB"
                     /locus_tag="VFG22_04385"
     CDS             2794..3396
                     /gene="rdgB"
                     /locus_tag="VFG22_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HET6415509.1"
                     /translation="MRILLATMNAGKLREYERLLAGVPGLELETMASLSAPIEVVEDR
                     DTFHGNALKKATEIAKVAGVICLADDSGLEVDFLGGRPGVHSARFAGERASDDQNNAK
                     LLDALRDTAESQRTARFRCAIVVVNPTGQELAAVDGVCEGRIAREASGAHGFGYDPLF
                     VPDGHSETMAELGPETKNRISHRAEAAAKLAPLLQQLRNS"
     gene            3442..3515
                     /locus_tag="VFG22_04390"
     tRNA            3442..3515
                     /locus_tag="VFG22_04390"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3476..3478,aa:Pro,seq:tgg)
     gene            complement(3541..4623)
                     /locus_tag="VFG22_04395"
     CDS             complement(3541..4623)
                     /locus_tag="VFG22_04395"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET6415510.1"
                     /translation="MALRKLANSWQFDFTLQGYDRQRQAGFKTKSEAREAERQRRTDL
                     ISGRKRILFADAYTQYMSATRMKALGRDHWERCWPDIKPVLGHRFIEEVDTSALDLLK
                     TRLPSHLAPSSINHRLSLVRTILRFMWKRGLLASVPYVPMESVPTTEPVWYGEEERDR
                     LLDGMFRLQPRWYLFFYLTTRLGLRTGEVYAVAKSRIRDIPPQLIVDRAVQRGNKERP
                     ALLGTRKNNRSLKLSLTQDMVDAIRWHIGQRYSGEEFLFSIDGTFPRHLDSHKRPLRT
                     VQRALGLRELSHHQIGRHSVASQAATSGHSIKAIQAQLGHQSAQSTHLYAHLGDQAQL
                     RLVEALRPVSPPHVNVRSTAPDLQDR"
     gene            complement(5891..6829)
                     /locus_tag="VFG22_04400"
     CDS             complement(5891..6829)
                     /locus_tag="VFG22_04400"
                     /inference="COORDINATES: protein motif:HMM:NF040247.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5914 domain-containing protein"
                     /protein_id="HET6415511.1"
                     /translation="MGQPIEDSPDWQQANPKRIDKALCRALALPSGGWFVVGASRGIT
                     TAPRCVRICDRHLVAWRTSQGVRLAPNECPHMGASLAEGLVHDDRLVCPWHGLAIGDE
                     GHGSWKPLPTFDDGVLTWVRLDDEEPGVALSDQPFMAPRPKTFIDGVIQLWAECEPRD
                     VIANRLDPWHGTHFHPYSFGRLKVIDLTEEAVTVRVAKRVLGPFAVEVDATFHCPDPR
                     TIVMTIIGGEGEGSVVETHATPICPGRTAIIEATLATSDRYQFKMAQLVAGFVRPFME
                     RSARRLWVDDAAYAERLYRLRDERPGRYGRHRRPSA"
     gene            complement(6871..8376)
                     /locus_tag="VFG22_04405"
     CDS             complement(6871..8376)
                     /locus_tag="VFG22_04405"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6415512.1"
                     /translation="MTSAPTGPVPPADAPQENVPAGTSAVVVGGGIAGIAAATVLAER
                     GVVVHLVERGSQLGGRAGAWTETLSDGQSFEMERGFHAFFRQYYNLRALLRRVDPELA
                     HLASVEDYPVLGPDGTSESFAKLVRRSPFNVIQLTHRTKYMSFGDLFKVNRKPALDMF
                     RFDSEWTYAEYDDKSASDYFDSLNFPDKARSMLFNVFAHSFFNPEENMSAAELLMMFH
                     FYFTRNPEGLLFDVAQRPFSTAYWEPFAHYLRERGATVECGCVASRLRRRQGRWVVDT
                     SSGEIVSDMVVLAVTVPALKALVDASPELHKESWLQRVHSLGLTNPFAVWRLWLNKPT
                     EEGRAPFAGVTEYECLHNISLYHLFEDESREWAEKCGGSVVELHAYALDPQLGADDIK
                     GHMLRGLHDLYPETRDAEIVEERFLLDQDCPSFAPGSDSARPTVETPFPGLCLAGDSI
                     KLPFPTALMERAAASGMMAANCLLAPLGVVPEPITTVPRRGLLRARRHDRA"
     gene            complement(8373..9212)
                     /locus_tag="VFG22_04410"
     CDS             complement(8373..9212)
                     /locus_tag="VFG22_04410"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6415513.1"
                     /translation="MRKPSTSAPIDGAPRENGHFALALRANGASQAAELPVSSLREMN
                     DLQLRRTQVPGEFDRIAHRYDLLNRLNPGYRKHLHWSAERMELARNANVLDLCCGTGL
                     STEALVDAYPEATITGLDASSGMLELAKTKPQLERVRFVCGDAMDPRSAGLAPARYDG
                     VFMAYGIRNMPDPDLCLGRILELLKPGGTIAFHEYSVADSRVSRAIWNLVAGTIIIPF
                     GALTTGSSKLFRYLRSSVNHFDGVEAFKKRLLAAGFIDVREEPMDGWQRGIVHTFLAA
                     RPQ"
     gene            10469..11677
                     /locus_tag="VFG22_04415"
     CDS             10469..11677
                     /locus_tag="VFG22_04415"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HET6415514.1"
                     /translation="MRHVVGLLRVAALSLLLSVGCREAGGEGLCEGVDCDDGNECTQD
                     LCDPTDGMCDYAPVDDGTACEEGNECSTGMCGEGMCAPVADGTVCGNDAGTCQQGSCQ
                     VACREQGIRDAIAAGGGPYTFDCDGPTTVVTTAETVIDNDVTLDGEGKLMVDGDEEHR
                     VFSIPEGVAAELRGLLVTGGNSGGHGGGIFNEGDLTVTSSTVSGNSALIGGGIFNTGT
                     LTLTNSTVSHSTAESAGGIANTGTLTLINSTVSHNTAESGDGIYGGAGGIQNGGTLTL
                     SDSTVSGNAAEVGGGIYNFGELTLTNTLVDNDCGGGTALSGGGNLESPGATCGFDQPT
                     DQVNVTAEQLNLGPLADNGGPTETHALLPGSVAIDVIPEADCQLDTDQRGEPRPAGAG
                     CDVGAFEVQP"
     gene            11690..12454
                     /locus_tag="VFG22_04420"
     CDS             11690..12454
                     /locus_tag="VFG22_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415515.1"
                     /translation="MRFLFGFMCVLVLAVACGQGDSVPLCQEEGRGSSLLAACVSGPR
                     VEEPTYFPPDLLEVRIEGVVEEMGQGGTSECFDLWYASLGDRIEADDPELDEARWVSF
                     RTAEGELHRVEVIAPGGFQWSASVGESLVVDLSQRWRGFPPTVSSLEIRAKAEKLAYW
                     MGVAWKLEELGPPDELELADGGEGCLVIGPDCNARWEERSLSASVDGETEVVDYGAQL
                     SIGSLTVLNHAYEVQVGQVDCEDYFVGTAEVATWPK"
     gene            complement(13023..>13427)
                     /locus_tag="VFG22_04425"
     CDS             complement(13023..>13427)
                     /locus_tag="VFG22_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415516.1"
                     /translation="RRDTLVRNANTVPACSIGATRRVSKQAAEKITAHDAVGAVWAVG
                     IDAPYVRVALVERARAPIAGAFRNLPELADPLPVAKLIGSTEVVWLVADDAFAELLTS
                     TYAVKAGTLPQILRPTQSSIFPVATIVVAVFA"
BASE COUNT         2539 a         3883 c         4422 g         2583 t
ORIGIN      
        1 ccccaagccc cagcgtatcg cgtcagaatc aggggttgtt ttcgctctgt ggtgattgct
       61 aggactagaa cctcgccagg ttccgctacc aggtttagga tccacgcatg actcgtccgg
      121 ctcgccggaa atggctcacc atcccgactc gcatcgtgct tggctacatc ctggtgctcg
      181 tcgccttcgc tgtggtcggt agcttgagct tcgtccagca ccatcggacg tcgcagacgc
      241 tcgggctgct tgaggacgga taccttcctt tggcgctcac gttgagtcag gccaaggcga
      301 cgcagagcgt gtacgcgaat ctcgtggagc gcgcgcttca atcggaacgg agcgcgccga
      361 ccaagtcctg gcttcgcgcg gccctgcgga ttcgtcctac tacgcttcag cgcgcctatg
      421 acggcgtgga acaggcggag acgctggcgg aggagcttgg ccagcctgcg ccgcttcgct
      481 cggttcgtcg cgagctcgat gcgatcgcta ccgcgtacac ggagatcgac gccaagtacg
      541 aggagctctt caatcaagtc gacggtgacg aagcagctgc tgaggctctt ctcacgaagc
      601 tccgaagcac cgagcaacgg atcgagagga actacgatcg cgcatcgcaa agcgtccagc
      661 agcgcatttc gcgaacgagc gcactggcgg ccgcacagtc ggagcgcgcc acgttcctct
      721 tgggactgct ggggctgctt gcactgctcg tgggcattgc ggtgacgttc ttgtcgcagc
      781 gcctgcttcg gccgattcct ttgttgcatc agcgtgtcgc cgccgtggcg catggagatt
      841 tgtcgagcgc cgtggacatc aagcgcgacg acgagctcgg ccagctcgtc gccgagttcg
      901 agcgcatggt gcaggccttg gccgcgcgcg atcagagcct tcgggaggca gcgctcgcgg
      961 agcagcgact ccagcgcatg caggttcaga tcgtcgaggc tctccgcgca gcgattctcg
     1021 tgatcgaccg cgaccggatc attcagacga cgaatccagc cgccgacgtc atgctcggag
     1081 tcagcgcgcg cgaagtggga gcatcgatcg acgaaacgtc ggtgcttcgg cgattccccg
     1141 cgctcgggcg cgctattgcc gaggttgcaa aggggggcga tgcggtgacg ctcgagggtg
     1201 aacgtatttc gggattcacc gagcatctgg tcgacgtgct cgtcacgccg tttggcgacg
     1261 aactggggga gggcggttcg cgcggcgtgc tcgtcgttgc agacgacgtc tccgacgcgg
     1321 tcgcgacgaa gctgcggctc attcaaaccg agcgccttgc ggcaattggg cgaatggcag
     1381 cacacgtgac acacgaagtc cgcaatccgc tctcgagcat cgggctgaac gtggacatgc
     1441 tcggcgacga gatccacggc gccggtccag agagcaagca tctactggac tcgatccagc
     1501 gggagctcga gcgtctcgag agcatcaccg aggagtacct tcgactggcg cggctccctg
     1561 agccctccct cacgccggag gacccgacgc acctcctccg tgacatggcc gagttcgttc
     1621 gactcgagat ggacaccgct ggcgtcgagc tccgattgga cttggctgcg cagttgcctc
     1681 ctgtcgcgat ggacgagccc caacttcggc aagctctgct caaccttctg cgcaacgcgc
     1741 gcgaggccat gcccgatggt ggaaccgcca agctcgaggc catgcgctac gacgggggtg
     1801 ttcgtattca agttcacgac gaggggacgg gcatcgccga ggatgagcgc gagcacgtgt
     1861 ttgatctctt ctacactacc aaggagcggg gcaccggtct cggccttccg ctgacgcagc
     1921 agatcgtggt ggcgcacgga ggaagcatcg cgtgcaaagc ccgccatccg cacggaacga
     1981 ccttcgaggt gtggttgcca gcagccgcac aaacgactac tggttcgccc gtgcggaggg
     2041 ttggatgacg agagtagacg ggcgcgcata cgacgcgctc agaccggttt cgatcgatgc
     2101 gaacgtgcag cgcaacccgg agggttcggt gctctatcga tgcggcggca ccacggtgtt
     2161 gatcgccgcg tcggtctcgg atcgggtgcc cgactggatg cgcgataccg ggcgtggctg
     2221 gctcacggcc gagtatgcga tgcatccgcg cgccaacccc gaacgccagc ggcgcgacag
     2281 ccgaaagggc gccatagatg ggcggagctc ggagattcag cgtttgatcg gccgcgcgct
     2341 gcgggctgcg gttgatttcg agaagcttgg cgagcggatg atcacggtcg actgtgacgt
     2401 gctcgatgcc gatggtggga cgcggaccgc ctcgattacc gggggtatgg tcgcgcttgc
     2461 aatggcactc gaccggcttc gtgcgacagg tttggtcgac aggggcgtgc tccatgcacc
     2521 aattgcagcg atcagcgtgg gtttgctcga agcgcgtccg atgctcgatc tttgctacac
     2581 cgaagaccgg gacgccgagg tcgacttgaa cgtggtcggg acgccggccg gtggcgtcgt
     2641 cgaagtacaa gggactgccg agggagcacc gatccttcga gagaagctcg accagatgat
     2701 cgacttggct ctcttcgcca tgccggcact cgccgaagaa cagcggcgcg cgcttcgtgc
     2761 ggtcgatgtc gacatcgagc ggctgctgcc ctgatgcgaa ttctcctcgc cacgatgaac
     2821 gccgggaagc ttcgcgagta cgagcggctt cttgcaggcg ttccggggct tgagctcgag
     2881 acgatggcgt cgctttccgc gccgatcgag gtcgtggagg accgcgacac gtttcatggg
     2941 aatgcgctca agaaggcgac cgagatcgcc aaggttgcag gggtcatttg cctcgccgat
     3001 gacagcggtc tcgaggtcga cttcctcggc ggtcgacctg gggtgcactc ggcaaggttc
     3061 gctggggagc gcgcatccga cgaccagaac aatgccaagc tactcgacgc cttgcgggac
     3121 actgcagaat cgcaacgaac cgcacgattt cgctgcgcca tcgtcgtcgt caacccgacg
     3181 gggcaggagc ttgcggccgt cgacggcgtg tgtgaaggcc gaatcgcgcg agaagccagc
     3241 ggagcccacg ggttcggcta cgaccccttg ttcgtccccg acgggcactc ggagaccatg
     3301 gccgagctcg gccccgagac caagaaccga atcagccacc gcgccgaagc cgcggcaaag
     3361 ctcgcgccct tgctgcagca gttgcgaaac tcctagccat cggctacccc atgccgccgg
     3421 accgcagtag attgaaggtc acggggtgta gcacagcttg gtagtgcgca tgctttggga
     3481 gcatgaggtc gctggttcga atccagtcgc cccgactaat catttcaagg acttgcggcg
     3541 ctaccgatct tggagatcgg gggccgttga cctgacgttg acatgaggtg gagaaacggg
     3601 ccgtaacgct tcgactaatc ggagctgcgc ctggtcgccg aggtgggcat agaggtgcgt
     3661 gctctgcgcc gactggtggc cgagctgggc ctggatcgcc ttgatggaat gaccgctggt
     3721 ggcggcctga ctggccaccg agtgacggcc gatctggtga tggctcagct cgcggagccc
     3781 tagcgcccgt tgcaccgtgc gcagtggtcg cttgtggctg tcgaggtgcc tgggaaaggt
     3841 tccgtcgatg gagaaaagga attcctcccc cgagtaccgt tgcccgatgt gccaacgaat
     3901 cgcgtcgacc atgtcctgcg tgagcgacag tttcagcgat ctgttgttct tgcgggtccc
     3961 aagaagcgcg ggccgctcct tgttgccccg ctgcaccgcc cggtcgacga tgagctgcgg
     4021 cgggatgtcc cgaatacggc tcttcgcgac ggcgtatact tctcccgtcc gcagaccgag
     4081 cctggtcgtt aggtagaaga ataagtacca gcgcggctgg aggcggaaca tgccgtcgag
     4141 aagacggtcc cgctcctcct cgccgtacca gacgggttct gtcgtgggga cgctttccat
     4201 cggcacatag ggaaccgatg caaggagacc ccgcttccac atgaaccgaa ggattgttct
     4261 caccagggac agtcggtgat tgatcgagct cggtgcaagg tggctgggca acctggtctt
     4321 gagcaggtcc agcgccgagg tatcgacctc ctcgatgaac cggtgcccga gcacaggttt
     4381 gatatccggc caacagcgct cccaatggtc gcggccgagg gccttcatgc gggtcgcgga
     4441 catgtactgc gtgtacgcgt ccgcgaatag gatgcgcttt cgacctgaga tcagatcggt
     4501 gcgccgttgt cgttcggctt cgcgcgcctc cgacttggtc ttgaacccag cctgtcgctg
     4561 gcgatcgtag ccttggagtg tgaagtcgaa ttgccatgag ttggcgagct ttctaagggc
     4621 catgcggctc gttcctttcc aacaggaact tccgcatcgc catgtccggg ttcgtcagga
     4681 agaggtctaa catatcccgg cggaaccggc gtacccgcgg acgtccgtaa accggaatcc
     4741 gtcctcccga gactaggttt cgtagatgtc ggatcgacca gccggtgtgg cgtgcggctt
     4801 gggcataagt gagccacgtt ccgactctgt gggtgggtgt ttccacggcc tctagtcctc
     4861 cgtcctggga atgaactcgc ggggcttgcc ggggattcgc ggccagactt gcggggagga
     4921 ttcgagcgga gctgatcgct tcggagcaat tgcgttctcc gagatgggct tcgtcattgc
     4981 ctctggaggg gcgcctcgag gcatgctgag tccgtgctga cggcagtacc gccagaacgt
     5041 ctggtaggag gcttcgaacg gcggtgtggc gcgtcggcac gccgtccaga cgcctttgat
     5101 tgagtagcca gactgcagcc aggctgcgat ctggtccctt cgctgaagga agcaggcata
     5161 cggttcgtta cccggccgct ctgccggtgg gtagttcggg caaggttgtg gtttcatcga
     5221 ccaagttatc ggttttgggg accaaaagta ttctcggaga aggcattttt tttcgacgac
     5281 cgcgctgagc tcagtcggaa gcgactgcga cccgccggat ctcgttggag ctcactgcaa
     5341 ctgcgtggga gtcggtgaga ccggttagag ctcggcgata ccgactcagg ccacatcgcg
     5401 gcgagttacg gtcaatcatg acaagagatt tctttgcact cttactccca gcgagtttca
     5461 ccttgtttca gacgtttgca tgcgcttgca ataacgaacc cagctcgttt ccggcaaggt
     5521 ggagttttgt ctgccaacaa aactgcatgc gccctcacgt gctgatcgtg tgatcgatcg
     5581 cgcgggtcac acgctcaacg ccttcggctt attcgcgcgc gaagcgcaag agactctaga
     5641 ggcgaaaaga tctgatctgg gcgctcgcga ctagcgtatg ggtgatctga tacatcgact
     5701 cacgtgcggc tcacttcgag ccgataagct gatagccgct acaaacgcct tctttcctga
     5761 aaccgaaagt agacagataa ggttttctgc gcaattcagg agctgagtga gcctccggtt
     5821 tgcgctcatc tcggcgtggc cgctcctttg gagatgttac cgaggcgtcg attgtcgttg
     5881 cgactgaggc ctatgcgctt ggacgacgat gacgaccgta gcgtccgggg cgctcgtcgc
     5941 gcaggcggta gaggcgctcg gcgtaggcgg catcatccac ccacaggcgc cgcgccgagc
     6001 gttccatgaa cggacgaacg aaacctgcga cgagctgggc catcttgaac tgatagcgat
     6061 cggaggtcgc cagggttgcc tcgatgatgg cggtgcgtcc ggggcagatc ggcgtggcat
     6121 gggtttcgac cacggagcct tcgccctcgc cgccgatgat cgtcatgacg atggtgcggg
     6181 gatccgggca atggaaggtg gcatccacct cgacggcaaa gggaccgagt actcttttcg
     6241 cgacgcgcac ggtgactgct tcctccgtga ggtcgatgac tttgaggcgg ccgaaagagt
     6301 agggatgaaa gtgcgtgccg tgccaagggt ccaagcgatt ggcaatgacg tcccgcggct
     6361 cgcactccgc ccaaagttgg atgacgccat cgatgaacgt cttcggtcga ggggccatga
     6421 agggttgatc gctgagggcg acgcccggct cttcatcatc gaggcgcacc cacgtgagaa
     6481 caccatcatc gaaggtgggc agcggcttcc acgagccgtg accctcgtcg ccaatagcca
     6541 gaccatgcca agggcagacg agccgatcgt catggaccag accctccgcg agcgacgcgc
     6601 ccatgtgtgg acactcgttc ggcgcaagac ggacaccctg gctggttcgc caggcgacca
     6661 agtgtcggtc gcagatgcgc acgcagcgag gcgccgtcgt aatgccgcga gaagccccga
     6721 ccacgaacca accgccggaa ggcagtgcca gtgcccggca gagggctttg tcgatgcgct
     6781 tggggttggc ttgttgccag tcggggctgt cctcaatggg ctgccccacg gattcatcga
     6841 gtcgtctgcc gaaggtccaa agtggtttca tcacgctcgg tcgtgtcttc gggcccgtag
     6901 aagaccgcgc cggggcacgg tcgtgatcgg ctccggaacg acacccagcg gggccaacaa
     6961 acagttggct gccatcatgc ccgaagcggc agcgcgttcc atgagcgcgg tcggaaaggg
     7021 aagtttgatc gaatcgccag cgagacacaa tcccggaaac ggagtctcga ccgtaggtct
     7081 cgcggaatcg gagcccggag caaacgacgg gcagtcttga tcaagcagga agcgctcttc
     7141 cacgatctct gcgtcgcggg tttccggata gagatcgtgg aggcctcgga gcatgtggcc
     7201 cttgatgtcg tcggcaccga gctgcggatc cagcgcgtag gcgtgcagct cgaccaccga
     7261 tcccccgcac ttctctgccc actcccggct ctcgtcctcg aacaaatgat agagggagat
     7321 gttgtggaga cactcgtact cggtgacgcc cgcgaacgga gcccggccct cttcggtcgg
     7381 tttgttcaac cacaatcgcc agaccgcgaa gggattggtc agcccgagcg agtggacgcg
     7441 ctgcaaccac gattctttat gcaactccgg tgaagcgtcc acgagcgcct tgagggcggg
     7501 cacggtcacc gccaagacca ccatgtcgga gacgatctcc ccagagctcg tgtcgacgac
     7561 ccaacggcct tgccgacggc gcaggcgaga agccacacag ccgcactcga cggtggcacc
     7621 gcgctctcgg aggtagtgag cgaaaggttc ccaataggcg gtggaaaacg gcctttgggc
     7681 gacatcgaag aggagtcctt cggggtttcg ggtgaagtag aagtgaaaca tcatcagcag
     7741 ctcagccgcc gacatgttct cctcgggatt gaaaaacgaa tgagcgaaga cgttgaacag
     7801 catcgaccgg gctttgtccg gaaagttgag agagtcgaaa tagtcactcg cgctcttatc
     7861 gtcgtactcg gcgtaggtcc actccgaatc gaaacggaac atgtccagcg cgggtttcct
     7921 attgaccttg aacaggtctc caaagctcat gtacttcgtc cggtgagtga gctggatgac
     7981 gttgaacggt gaccttcgta ccaacttggc aaaggactcc gacgtgccgt ccggtccgag
     8041 gacaggatag tcctcgacgg aggcgaggtg cgcgagctca gggtcgaccc gcctgagtag
     8101 ggcacgcaga ttgtagtact ggcgaaaaaa ggcgtgaaag ccgcgctcca tttcgaagct
     8161 ctggccgtcg ctcagcgtct cggtccaggc tccggcacgg ccaccgagtt ggcttccgcg
     8221 ctcgaccagg tgcacgacca cccctcgttc agccagcacc gtcgccgcag cgattcctgc
     8281 gatcccaccg ccgacgacga ccgcactcgt ccccgcgggc acgttctcct ggggtgcgtc
     8341 cgcggggggc acgggtccgg tcggcgctga ggtcactgag gcctcgccgc caagaaggtg
     8401 tgaacgatgc ctcgttgcca gccgtccatg ggctcctctc taacgtcgat gaacccagcg
     8461 gcaagcaaac gcttcttgaa ggcctccacg ccgtcgaagt ggttcacgct gctccgcaga
     8521 tagcggaata gcttggagga gccggtggtc agagcaccga aggggatgat gatggtgccc
     8581 gcgacgaggt tccagatggc tctactgacg cgggaatcag ccacggagta ctcgtgaaaa
     8641 gcgatcgtgc cgccgggctt caggagctcg agaatgcggc cgagacagag gtcggggtcg
     8701 ggcatgttgc ggatcccgta agccatgaag actccgtcgt agcgtgccgg cgctagtccc
     8761 gcagaccgag ggtccatggc atctccacag acgaagcgaa cgcgctcgag ctgtggcttg
     8821 gttttcgcaa gctcgagcat gcctgaggaa gcatctagcc cggtgatggt cgcctcggga
     8881 tacgcatcca ccagggcctc ggtcgataga cctgtgccgc agcagaggtc gaggacgttt
     8941 gcattgcgag caagctccat ccgctcagcg ctccagtgca ggtgcttgcg atagcctggg
     9001 ttgaggcgat tcagcagatc gtagcggtgc gcgattcgat cgaattcacc gggaacctgg
     9061 gtccttcgga gttggagatc attcatttcg cgcaaggagg atacgggcag ctcggccgct
     9121 tgagaagcac cgttggcacg gagtgctaag gcaaagtgcc cgttttcgcg cggtgccccg
     9181 tcgatcggcg cagaggtgga tggtttgcgc atgcaggcct tcatggacgg agacgcaatc
     9241 aagacaggcg ttccaggttg gacccatagc gtgctacgcg agaccgtcga catcaccttc
     9301 gccgcgctgc agttgacttc ttaggtacga ggactctctt agcccatcgg gctcatcgta
     9361 aacgcccaat tgcgcggtac tagcttctta tgtcgcctgg ggcgcgagcc gaaaagggag
     9421 ttcatgtcga ctctcagccg cgtgggtcgt gtgcgtggca ggatgtgtat tcacaagctg
     9481 ccacaacccg gaccggccca ggattgggtt cgttgacctg acgttgacgt ggcgatgtga
     9541 aatcggatga tatgaagtga cgaaccgcaa acacggcgat gcgggcaagt cccggtcatg
     9601 tttgggtttc tgtttgtttg cgtgatgttg tgcttgctcc gcggctttgc tttgggagca
     9661 tgaggtcgct ggttcgaatc cagtcgcccc gaccagtgat tacgagtggt taggcgtttc
     9721 gcgattttcg atttcggctc tcgttgacca agtattacaa cgatgtggcg gcgacgccgg
     9781 ttcgagcgca ctgaggaggc gcagttgagc actaatacca aggtgcgcgt acacctcggt
     9841 gcttcaagcg gagcggtgcc agagttgagc ctggatggct tacgtcgaag tcaagcgaag
     9901 agctgacatg gaaagccatc atgtatggcg cagatatttc gtcggagatc gccactgcta
     9961 cgcgtcaatc cgtcaagttc tcccgaaaaa ccggcttcgt gtggttggca cggctgatgc
    10021 agttgcatag ctccgcttat tgaattgggc aggtcggtta tgcgttgcgt attccgattc
    10081 ctaatggcgc tcgccctgtt tgcctttgct gcttgcgagg gccgaggagt tgattgcgcg
    10141 ggtccagcaa ctgaattcac gggtgagtgg gaaatcacct caacggtcgc gtccgacaac
    10201 cgtgatggga gaatggccca gacgtttccc atgatcattg cacaagacgc cactgcgttg
    10261 acggctgaga cgtctgactt ggatttcagc ggaacgatcg gcggcaacca gatccagatg
    10321 agcggcagct ttcccgagga tggcgggacc gtgacggtga acaccatgct gactgtctcc
    10381 tgcgatggag attccttgca gggcactacc accccccatc cccaaagttc gcctgcgtgg
    10441 cacgcgattc tcagggcgga ggtgggtcat gcgtcacgta gtcggacttc tacgtgttgc
    10501 cgcactgagc ctcctgctgt cggtcgggtg ccgtgaagcc ggcggcgaag gcctgtgcga
    10561 aggcgtcgac tgcgacgatg ggaacgaatg cacccaggat ctatgcgatc cgacggacgg
    10621 tatgtgcgac tacgctccgg tcgatgacgg cacggcttgc gaggaaggca acgagtgcag
    10681 cacggggatg tgtggagagg ggatgtgtgc gcccgtcgct gacggcacgg tgtgtggcaa
    10741 cgacgcgggc acgtgtcagc agggtagctg ccaggtagcg tgcagggagc aaggcatacg
    10801 cgacgccatt gccgcgggcg ggggacctta cacgtttgat tgcgacgggc cgacgacggt
    10861 ggtgaccacg gcggaaaccg tcatcgacaa cgacgtcacc ctcgacggcg aaggtaagct
    10921 catggtcgat ggcgatgaag agcatcgtgt gttttcgatc ccagagggcg tagcggcgga
    10981 gttgcgcgga ttgttggtga ctggcgggaa ctctggtggc cacggtggcg gcattttcaa
    11041 cgaaggggac ctgacggtga ccagcagcac cgtgtcgggg aatagcgcgc tcatcggcgg
    11101 cggcatcttc aacaccggaa cgctgacgct gaccaatagc accgtatcgc acagtaccgc
    11161 ggagtcggcc ggcggcatcg ccaacaccgg aacgctgacg ctgatcaaca gcaccgtgtc
    11221 gcacaatacc gcggagtcgg gcgacggcat ctacggcggc gccggcggca tccagaacgg
    11281 cggaacgctg acgctgagcg acagcaccgt atcggggaat gccgcggagg tgggcggcgg
    11341 catttacaat ttcggggaac taacgctgac caacaccttg gtcgacaacg attgcggtgg
    11401 tgggacggcg ctgtcgggtg gaggcaacct cgaaagcccc ggcgccacct gcggctttga
    11461 ccaacccacc gatcaggtca acgtcaccgc cgagcaactg aacctcggac cacttgcaga
    11521 caacggcggg ccgaccgaga cgcacgcgct gctgccgggc agcgtggcca tcgacgtcat
    11581 tcccgaagct gactgccagc tcgataccga ccagcgcggt gagccacgac cagcgggagc
    11641 tgggtgcgac gtgggtgcgt ttgaggtgca gccgtaaggg aggttgctta tgcgtttctt
    11701 gtttggattc atgtgcgtgc tcgtgctggc cgtggcgtgc ggccaagggg acagcgttcc
    11761 gctctgccaa gaggagggtc gcgggtcttc cctgctcgct gcctgtgtct cgggtccgag
    11821 agtggaggaa cccacgtact ttccacctga ccttctcgag gttcgcatcg agggcgtcgt
    11881 ggaggagatg ggacaaggcg gaacgagcga atgcttcgac ctctggtacg caagcctcgg
    11941 cgatcgtatc gaagccgatg atcctgagct cgacgaggca aggtgggtca gtttcaggac
    12001 cgccgagggc gaattgcatc gagttgaagt gatcgcgccc gggggatttc aatggtccgc
    12061 atcggttgga gagagtctcg tcgtagacct ttcgcagcgc tggcgtggct tcccccccac
    12121 tgtgtcgagc ttggagatcc gagcgaaagc cgaaaagctc gcatattgga tgggggtcgc
    12181 ctggaagctc gaagagctgg ggccgcccga cgagcttgag ctcgcggatg gcggtgaagg
    12241 atgcctggtg atcggtcctg attgcaacgc caggtgggag gaacggagcc tcagtgcgag
    12301 cgtcgacggc gagaccgagg tcgtggacta cggagcgcaa ctgtcgatcg gctcgctcac
    12361 tgtcttgaat cacgcctatg aagtccaggt cggccaagtc gattgcgagg actacttcgt
    12421 cggtacagca gaggtcgcga catggccgaa atgaccacta gagcgtgatc tcacccggtt
    12481 gctcgtgtgt cgcgtgcgct catggaactt cagcgcaggc tcttcgggta cgggctcaga
    12541 cggcgacgcg ggccgagaat tgctgccact gctctaggtt gtggagatgc agcggcaggt
    12601 catatagtgc aaacatttcg ccggccaccg tcatcactgt gcttcgcggc atgcgattcg
    12661 ctggaaaaac tgcgctcaat agcgtggcac gcctgatgca aggcgttggc atagtagcct
    12721 ttaacgggag atgctgtttg cgctacgtat tccgattctg gctcgcattc gcgctcggcg
    12781 tggtgctgtt aggggggtgc ggtgaagacc gtattccgtg cgagagtgtc gaagattgcc
    12841 ccccggtcga cccgggcgag tgcgagatac atccgctctg ccaacagggt tcctgccggt
    12901 actacagtga tcgggtcgga gccggctgcg cccgaagcga tggcagcgtg ggcgtatgcg
    12961 aacaccccat caccaaaccc agctctaaag ccgacggctg cacagaagaa tcaggaccga
    13021 tttcatgcaa acacggctac aacgattgtt gcaactggga agatggagct ctgtgtcggt
    13081 cgcaggattt gcgggagggt acctgccttg acggcgtatg tggaggtgag cagttctgcg
    13141 aaggcgtcgt ctgcgacgag ccagacaacc tctgtggaac cgatcagttt tgcaactggg
    13201 agggggtctg cgagttccgg caagttacga aatgccccag cgatgggagc acgtgcacgt
    13261 tcgacgagtg cgaccctgac atagggtgcg tctatcccca ccgcccagac ggcgccgact
    13321 gcgtcgtgag cggtaatttt ttctgctgcc tgtttggaga cacgtcgtgt tgcacctatg
    13381 gaacatgcag ggacggtgtt tgcgtttcgg actaatgtat cacgtcg
//
LOCUS       DASRZF010000098         2301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2821097, whole genome shotgun sequence.
ACCESSION   DASRZF010000098 DASRZF010000000
VERSION     DASRZF010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2821097"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="VFG22_04430"
     CDS             <1..646
                     /locus_tag="VFG22_04430"
                     /inference="COORDINATES: protein motif:HMM:NF012725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6415517.1"
                     /translation="KLIDSGVHDPDVYFNLATTFAQSGDYARAILYFERAITLRPNDD
                     KASENLRAAEQALEEIRAEAEGEATIQRSSSISDAVYGGISEDGLAYALLIGNFLFFL
                     SLAWVSLRGRRKAWLYSLLLTSAIVLLFCAVGLGAKAGVFRDGPRAVALKDHVVLREG
                     PDPRARIRGDARAGDRGEVVDRDRDFVKLRVVSGIEGWTAKSSIGFIDPEHGVH"
     gene            636..1100
                     /locus_tag="VFG22_04435"
     CDS             636..1100
                     /locus_tag="VFG22_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6415518.1"
                     /translation="MSIDIPGQQNEDAARVRDFRSGETMTTHGATKILVADDDLEILA
                     LVARHLSALPAEIVEASDGEEALRLARREKPNLVVLDVMMPGMSGWEVCRAIREDESL
                     GQTGVIMLTGIGERLNEMTSPLYGADGFLDKPFELDDLSAKVREVLAGRDSS"
     gene            1148..2089
                     /locus_tag="VFG22_04440"
     CDS             1148..2089
                     /locus_tag="VFG22_04440"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET6415519.1"
                     /translation="MPWKRAVIILLAVAAASCSKKAEEAPAGEEEWPKLGLTEAQANE
                     VLVKVGDRTITVGEFADRLASQSPYLRARFESPERRKEFLDNLVRFELLVHEAKRRGF
                     EERPEIIRARRNAMIQQLVTSEVDQPLEESEITDEEVQAAYDANPQEFNRPAQVRASH
                     IFIKERPRALKLLVKAKTTDLAGFRKLAREESEDEKTKGDSGDLQFFEAEGEGPPPAS
                     IRKAAFALNKVGAVYPELVEDGDGYHIVMLTGKRAALTRTYEQSKRAIRHKLTRQRKD
                     AAMEALTERLRQMVEVDVDYSVLQDIKVDVDDLPPKP"
     gene            2086..>2301
                     /locus_tag="VFG22_04445"
     CDS             2086..>2301
                     /locus_tag="VFG22_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET6415520.1"
                     /translation="MRGVLAVAVLVLALAAGAADADVVERVVATVDNQAIFLSDLRTR
                     AVPFLPQVADATTETERKARLKELYEEL"
BASE COUNT          483 a          674 c          729 g          415 t
ORIGIN      
        1 aaagctcatc gattcggggg ttcacgaccc cgacgtgtac ttcaacctcg ccaccacgtt
       61 cgcccagtcc ggggactacg cgcgcgcaat cctgtacttc gaacgagcca ttacgctccg
      121 cccaaacgat gacaaggcgt ctgagaatct gcgcgcagcc gaacaggctc tcgaggagat
      181 tcgtgcagag gccgaaggcg aagcgacgat ccagaggagc agctcgatta gcgacgccgt
      241 ctatggaggc atttctgaag acgggctcgc atatgcactg ctcatcggga attttctgtt
      301 ctttttgagc ctcgcctggg tctcgctgag gggccggcgt aaagcttggc tgtattcgct
      361 gctgctcacc tctgcgatcg tgctgctatt ttgcgccgtg gggctcggcg caaaggcggg
      421 cgtctttcga gacggaccgc gagccgtcgc gttgaaggat catgtcgtct tgcgcgaagg
      481 gccagatccc cgagcccgaa ttcgaggcga tgcaagggcc ggcgaccgcg gcgaagtggt
      541 cgatagagat cgcgatttcg tgaaattgcg agtggttagc gggatcgagg gttggacggc
      601 gaagtcgagc atcgggttca tcgacccgga acacggtgtc cattgacatc ccaggtcaac
      661 agaacgagga tgcggcccgg gttcgcgatt tcaggagcgg tgaaacgatg acgacgcacg
      721 gcgcgactaa gattctcgtc gccgacgatg atttggagat attggcgctg gtggcgcggc
      781 acttgagcgc gttacccgcc gaaatcgtcg aggcttcgga cggtgaagaa gctctgcgtc
      841 tggcacggcg cgaaaagccc aatctggtcg tgctcgacgt gatgatgcct ggcatgagcg
      901 gctgggaagt gtgtcgcgcg atccgcgaag atgaatcgct cgggcagact ggcgtcatca
      961 tgctcaccgg aatcggggag cgcctgaacg aaatgacctc gccgctctat ggcgccgacg
     1021 gcttcctcga caaacccttc gagctggacg acctgagcgc caaggttcgc gaggttttgg
     1081 cgggccgaga ctcctcctga actttggcct cgttgccgtg gatgaggtat atgctacccg
     1141 gctgacgatg ccttggaaac gcgcagtcat cattctgctc gccgtcgcgg ctgcgagttg
     1201 ctcgaagaag gccgaggaag ccccggccgg cgaagaggaa tggccgaagc tcggtctcac
     1261 cgaggcccag gccaatgagg tgctcgtaaa ggtgggtgat cgaacgatca cggtcggaga
     1321 gtttgctgac cgcctcgcct cgcagtctcc ctatctgcgc gctcgcttcg aaagcccgga
     1381 gcgacgcaaa gaattcctcg acaatctcgt tcgcttcgag ctcctcgtcc atgaggcaaa
     1441 gcgccgcggt ttcgaagaga gaccggagat catccgagca cggcgcaacg ccatgattca
     1501 acagctcgtc acgtcggagg tggatcaacc gctggaagaa tcggagatca ccgatgagga
     1561 ggtccaggcg gcgtacgacg cgaaccccca ggaattcaac cgcccggccc aggtgcgcgc
     1621 gagtcacatc ttcatcaagg aacgaccacg ggctctgaag ctgctcgtga aagccaaaac
     1681 gaccgacctc gccggattcc gaaagttggc gcgcgaagag tcggaggacg aaaaaaccaa
     1741 gggcgacagt ggcgaccttc agttcttcga agcggaaggc gaaggtccgc cgcccgcctc
     1801 cattcgtaaa gcggcattcg cgctcaacaa agtcggcgct gtatatcctg agctggtcga
     1861 agatggcgac ggctaccaca tcgtcatgct gactggaaaa cgggccgcgc tgactcggac
     1921 atacgagcaa tccaagcgcg ccatacggca caagctcact cgtcaacgca aggatgccgc
     1981 aatggaggcg ttgacggagc gacttcggca gatggtcgag gtcgacgtcg actacagcgt
     2041 cctccaggat atcaaggtcg acgtcgacga cctgcctcca aagccatgag gggcgttctc
     2101 gccgtcgccg tgctcgttct ggcgctcgca gccggagctg ctgatgctga cgtcgtcgaa
     2161 cgcgtggtag caaccgtcga caaccaagcg atcttcctca gcgacctgcg gacccgtgct
     2221 gttccattcc tgccgcaggt cgccgatgca acgacggaga ccgagcggaa ggctcgcctc
     2281 aaggagctct acgaagagct g
//
LOCUS       DASRZF010000099         3320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3854925, whole genome shotgun sequence.
ACCESSION   DASRZF010000099 DASRZF010000000
VERSION     DASRZF010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3854925"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VFG22_04450"
     CDS             complement(<1..322)
                     /locus_tag="VFG22_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415521.1"
                     /translation="MSEPPTLLIPEIDEDRTLVETRNANLDCSLAELSGERGSSWPGA
                     DRNASASRGASSVRRKLGLRRCAEGIVVLGLVAGLAFVGYQQWRIAESIRKSISGFGN
                     TESPP"
     gene            complement(319..1233)
                     /locus_tag="VFG22_04455"
     CDS             complement(319..1233)
                     /locus_tag="VFG22_04455"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HET6415522.1"
                     /translation="MSEILINGHQEIYVEKRGRLYRTRSAFASEHALMSALRNLSQFV
                     GRELSSRQPILEARLPDGSRVEAIIPPAAPSGPVVCIRRFRQQQLTISRLIELGAFTP
                     STAELLYQAVAERHNVIVAGGSGAGKTSLLNALVAMAPKDERVVVIEDARELQLLLPH
                     VVHLEAQPADDRGRGAVTVRDLFKATLRMRPDRVVIGEIRGGEALDLIQAMTSGHRGC
                     LSTVHATLPIDTLSRLETMALMSDLEIPINVLRGQIASAVDTVIQVARHQDGSRRVSH
                     VAEVLGYDESHGYEIRYVFESPSLRAIT"
     gene            complement(1311..1904)
                     /locus_tag="VFG22_04460"
     CDS             complement(1311..1904)
                     /locus_tag="VFG22_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415523.1"
                     /translation="MAGCHHVSPADRTSLNAELSNISGEELFRMGLWHATSGDLLRAE
                     QYLVAARQRGHDARQVTYWLVRSCAAANRYQSALAHAQLYLRDHPQDWSLRLIAASVH
                     EALGELARAQSELERIVQAVPQTPLPHYRLALLYQVRESQQHRARTHLEAYLALTPQG
                     PHAAEARAVLDESVEVAKGPQLVPDPSAIEHESGVLR"
     gene            complement(1939..2496)
                     /locus_tag="VFG22_04465"
     CDS             complement(1939..2496)
                     /locus_tag="VFG22_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415524.1"
                     /translation="MSPTPERHRQVLLTLLVAVALLALTALLGDSEAPTAVGSLRFEH
                     PNLFLEGSLCSHPKRSLVTARRSEELAHVRADRYAYDPADGVRAVRLYAEAEACYRLG
                     GLNSSASRVRDAASLMILQLNTDYAAARLSLARSLEQKRWADALREVQRLLRLTHHLG
                     RNEYVEWLEHILRRATARATAASPQ"
     gene            complement(2493..>3320)
                     /locus_tag="VFG22_04470"
     CDS             complement(2493..>3320)
                     /locus_tag="VFG22_04470"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET6415525.1"
                     /translation="RPAVPRPSLGARGVKRARALRHSALFALTEPPLRALAAWIAILP
                     IAVLRAPCRRSLTHAGNYLGLSADELLAGCVLSGSAVGLGTWLVCNRTSLPWEVTIVA
                     LGIGAVAPWLRVLSVAQNRARSVSRALPGAVDLAAMCMSAGLDFPGAFRRIVESASNP
                     DEPVIEEFARVLQELDLGQTRRSAMEGLATRVPTEQVREFVNSVVQAEDKGSPLASVL
                     IVQAQSQRLRRSIAAEETASEAALMLLGPMTLIFLCVIVLLLGPVVVRFMTGGLGPS"
BASE COUNT          698 a         1015 c          986 g          621 t
ORIGIN      
        1 gaggaggcga ttcggtgttc ccaaacccac ttatcgactt ccggatggat tcagcgattc
       61 gccactgttg atatcccacg aaggccagcc ccgcgactag acccaaaacc acgatgcctt
      121 cagcgcaccg gcgaagtccg agtttgcggc gaacggacga agcgccacgc gatgcactcg
      181 cgtttctgtc agcacctggc caggacgaac cgcgttcgcc gctgagctcg gctagggagc
      241 aatcgagatt ggcgtttcga gtttcgacca aagttctgtc ctcgtcgatc tccggaatca
      301 gaagagtggg tggctcgctc atgtgatcgc tcgcagagac gggctttcga agacataacg
      361 aatctcatag ccgtggcttt catcgtatcc gaggacctct gcgacatgcg agacacggcg
      421 cgacccatct tgatgtcgcg caacctgaat gacagtgtcg actgcagagg cgatctgccc
      481 tcgaagcacg ttgatcggaa tttccaagtc gctcatcagc gccatggttt ccaacctcga
      541 gagcgtatcg atcggcaggg tggcatgcac ggtggaaaga catccgcgat gccctgaagt
      601 catcgcctgg attagatcga gcgcttcgcc gccacgtatt tcaccaatga ctactcgatc
      661 cggcctcatt cggagagttg ccttgaagag atcacgaacg gttaccgcac cgcgaccgcg
      721 gtcatccgct ggctgcgcct ccagatgaac gacgtgtggg agaagcagct gaagctcccg
      781 cgcgtcctcg atgacgacga cgcgttcgtc cttcggagcc attgcgacaa gagcgttgag
      841 aagagaagtc ttacctgcac cgcttccacc ggccacgatt acgttgtgtc tctcagcgac
      901 tgcctggtag aggagctccg cagtgctcgg cgtgaatgcg cccagttcga tgaggcgact
      961 gatcgtaagc tgctgctgtc gaaaacgcct gatgcaaacc actggaccac tcggagccgc
     1021 cggcggaatg atcgcttcga cgcgagaacc gtcagggagc ctggcttcca aaatcggctg
     1081 ccggctcgac aactcacggc ctacgaattg ggataggttc cgtagcgccg acatgagagc
     1141 atgttcggaa gcaaacgcgc ttcgtgtgcg atacaggcgc ccccgcttct cgacataaat
     1201 ctcctggtgg ccgtttatga ggatctcgct cacctcctct ccatccaagt accggccgat
     1261 cggcgccaac aaggaggaca ctgtctggcc aaaaacgtcg cgtgggacac tcaacgaaga
     1321 accccgcttt catgctcgat cgcgcttggg tccggcacga gctggggtcc tttcgcaacc
     1381 tcgacgctct catcgagcac ggcgcgggcc tcagccgcat gaggcccctg cggagtcagc
     1441 gcgagatatg cttctaggtg ggttctcgct cgatgctgtt gagactcgcg gacttgatag
     1501 agcaaagcga gcctgtagtg aggcaatggc gtttggggga ctgcttgcac gatgcgctcc
     1561 aactcggatt gcgcgcgtgc aagctcgcct agggcttcgt gaacgctagc cgcgatcaat
     1621 cgaagcgacc aatcctgtgg atgatcgcgc agatacagct gagcatgggc cagagcggat
     1681 tggtagcggt tggcagcggc gcaagagcga acgagccagt aagtaacctg ccgcgcatcg
     1741 tgtccccgct gacgcgccgc cacgaggtac tgctcggcgc ggagcagatc acccgacgtt
     1801 gcatgccaaa gacccatgcg gaagagctct tctccactga tgtttgatag ctccgcattg
     1861 agactcgtcc tatcggcagg cgatacgtgg tgacagccgg ccatgagccc tacacaagtg
     1921 agcagcaacc atctgcattc attgcggcga agccgcggtc gctctggccg ttgctcgtct
     1981 gaggatgtgc tcgagccatt ccacatattc gttcctccca aggtgatgtg tcagtcgtag
     2041 caaccgttgg acttcgcgaa gcgcgtccgc ccaacgcttc tgttcgaggg atctggccag
     2101 actgagtcgc gccgctgcgt agtctgtatt gagttgcaat atcatcagcg acgcggcgtc
     2161 gcgtacgcgg gaagccgagc tattgaggcc gcccagtcga taacaggctt cggcttccgc
     2221 gtagagccgc accgcacgaa ccccatcggc cgggtcgtag gcatatcggt cggcgcgaac
     2281 atgcgcgagt tcttcggacc gacgggccgt caccaaagag cgttttggat gcgaacagag
     2341 cgagccttcg agaaaaagat tggggtgttc gaagcgcaag ctgccgacag ccgtaggagc
     2401 ttccgagtcc ccaagcaacg cagtgagcgc cagcagcgca actgctacca acagcgtgag
     2461 cagcacttgg cgatgccttt ctggagtagg actcacgacg gacccaaacc tcccgtcatg
     2521 aacctgacga ccacgggtcc taagagaagc acgatgacgc aaaggaagat cagcgtcatg
     2581 gggcccagga gcatcagcgc cgcttcagaa gctgtctctt cagcagctat actcctgcgc
     2641 aatcgctgcg actgtgcttg aacgatgaga acactagcaa ggggactccc cttgtcttct
     2701 gcttgtacca cgctgttgac aaactcgcgc acctgctcgg ttggcactcg cgtcgcgagt
     2761 ccttccatcg cgctccgacg agtctgtccg aggtctagct cttgcaacac gcgcgcgaac
     2821 tcttctatta ccggctcgtc aggattcgac gcactctcca cgatccgacg aaaggctcct
     2881 gggaaatcca ggccagcact catgcacatc gccgctagat cgaccgctcc cggaagcgct
     2941 cgactcacac ttcttgcacg gttctgagct acgcttagga cgcgcagcca aggggcgacg
     3001 gcccctatgc ccaatgcgac gatcgtaacc tcccatggaa gcgacgtgcg gttgcaaacg
     3061 agccatgtcc cgagacccac ggcagatccc gacaggacgc agcccgcgag cagctcgtcc
     3121 gcggagaggc ccaagtagtt gccagcgtgg gtcaacgacc ggcgacacgg ggcgcgaagg
     3181 actgcaattg gaagaattgc gatccaggcg gcgagagcgc gtaggggcgg ctcggtgaga
     3241 gcgaagagcg cgctatgccg aagggcccgt gcccgtttga cccctcgggc gcctaagctc
     3301 ggacgcggga cagcgggcct
//
LOCUS       DASRZF010000100         6423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4542646, whole genome shotgun sequence.
ACCESSION   DASRZF010000100 DASRZF010000000
VERSION     DASRZF010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 6423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6423
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4542646"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VFG22_04475"
     CDS             complement(<1..364)
                     /locus_tag="VFG22_04475"
                     /inference="COORDINATES: protein motif:HMM:NF013885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415526.1"
                     /translation="MTPAAPVVLDRVLVAAFVRLFPLWVTLGGAVALVYPRGFVWFLD
                     YGLITPGLQIIMLGMGLTLELSDFSRVFRAPAPIVWGVVLQYTLMPFIGWGVGYLFQL
                     PTAFAVGLILVCCCPGGTA"
     gene            405..1472
                     /locus_tag="VFG22_04480"
     CDS             405..1472
                     /locus_tag="VFG22_04480"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET6415527.1"
                     /translation="MFRPPTDDVRIEGLRPLIPPAILMEEIHPGEEAIQHIAEARQQI
                     SDIVHGEDDRLLVVVGPCSIHDPVAALDYAKRLKAIRDQHADDLFIVMRTYFEKPRTT
                     VGWKGLINDPNLDGSFDINHGLRVARGFLSTLGEMELPAGTEFLDPISPQFVADLVSW
                     GAIGARTTESQVHRELASGLSMPVGFKNGTRGTVVIAVEAVRAARHPHHFLSVTKQGL
                     TAIVATKGNLDCHVILRGGAGKPNYEPEFISSTVELLESAGLDGSVMVDCSHANSGKD
                     HERQAGVAIAVGQQIARGSTGVFGVMLESFLVGGRQDQEPGVELTYGQSITDACMSWD
                     ATVPVIEGLADSVRKRRNAGG"
     gene            complement(1495..2772)
                     /locus_tag="VFG22_04485"
     CDS             complement(1495..2772)
                     /locus_tag="VFG22_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415528.1"
                     /translation="MPSRACGLLGLFLALGCNVYDASLIDDGSAGVPNRPPASTSSPD
                     DAESLVFGLNDIFLRQSAETAARIGIDLDATATTGRDDATCQPPTVDGEVVGQAVVDG
                     SKGIDNAIGTTLLPTTGSALPCLEDNLALTQGRGIGTVLLWVRHWNGQPNDASVTAML
                     TTAVDGTSADPSLVGFADSDPLNLVYLSGSQDVEAPEPAWDGKDSWFLDPIDFSAGPT
                     GRASLELPKAEQVDAYVSYGRLVVPLMNGTEFKLIAGDGTLTSDGAMTVTVNGGVIMG
                     DIHDDRTRLEHGLFAGRFSLEKLGEATPQIGMCSINATVIETLFGQFADIHQFPEHDG
                     TGAECDAFSVGVTFNGVAGQIAGIAAFSRPQLAPCAQQTESIETDRCCPSQWLAGRTR
                     FETCDTREKIVKAARFDTLPSSIQVPVPEPEIR"
     gene            complement(2842..3843)
                     /locus_tag="VFG22_04490"
     CDS             complement(2842..3843)
                     /locus_tag="VFG22_04490"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET6415529.1"
                     /translation="MSARTGLLPFWKNYDRKTYLRAAILADELGYDSFWIPEAWGYEI
                     FSLLTEIAVHTKRIKLGTGIVNCFSRSPGLLAMNAATVDEISEGRLILGLGTSGQRVI
                     EGFHAREFKKPLTQLRDVIRVTNTLLSGKRLTESGAKLHEYRPFELAMKPLRSDVPIY
                     VAALKENSITSIGELADGWIPTFWPYTELKRGHAWIAKGAAKAGRDPSEITTAPFTTV
                     IPLGDAGAREARQIIAFYIGGMGDYYKELLGGFGYADACKRIDELYRDKKTRSQAADA
                     VPEAMIEALTIAGEPQYCISELQRRREYGIDLPILNLPSNLPWEMIEMFIRTMAPSA"
     gene            3959..4981
                     /locus_tag="VFG22_04495"
     CDS             3959..4981
                     /locus_tag="VFG22_04495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HET6415530.1"
                     /translation="MKIDAGLLVTNMNQIGVRVRELEEAGFDGCFTFEGPHEPFMPLV
                     LAAEHSKLEIGTGVAIAFARTPMLVANLAHDLQHYSGGRFWLGLGSQIRPHIESRFSM
                     PWDKPVSRMKEFVLALRAIFASWNSNERLDFRGEFYKHTLMPPIFNPGPATGGAPPIF
                     LGGVGPRMTEAAGEVADGMLLHPFHTERSLEKVMLPNVQRGLATSGRAASDFALCAQV
                     MVVTGENEAGFENARAMARNQIAFYASTPAYRSVLEMEGREELQPELRQLTKEGRWHE
                     MAARIDDELLGKIAAVGTPKEVASILHRRYGGFAERLAFSSPFPIPQACSAEIISALR
                     SHPKPA"
     gene            4978..>6423
                     /locus_tag="VFG22_04500"
     CDS             4978..>6423
                     /locus_tag="VFG22_04500"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HET6415531.1"
                     /translation="MTKPRCIPLDAVSDERVGGKAEGLVRLLGYGFEVPPGFVILGAT
                     PQNLPADLDEHYARIGGGRVAVRSSAIGEDAEDASFAGQYETVLNVEGTEGLRAAIED
                     CLQSLENARAVAYRDEKAGLTDVTMNIIVQQMVDARTAGVLFTANPVNARRDQVVVDA
                     VAGLGEALVSGHATPDHWLLMRDGSVLLSEVQGETPVLTTAELQLLLDGAVRAEERYG
                     APLDMEWAIDGKGAVRWLQARPITQLPADPNELDTDPNPRDVYTWCNIGEMMPGAVTP
                     LTYSVTGRGIDIGMQRAYRRMGAEVPPGEGVRYVGMFYGHLFINLSTLAELSAAVAGS
                     SKGQMCIALCGRDIEEVSEPQPAPAWRRALNGARYFAVLFGAAKHRRRLDALLDDLRL
                     PKLGTSHQLYRAIDERLPLVWRAYELHLMSSASSGALSPILLGILAKGGEPTQEHHAE
                     VAEMLAGAADVESADIAEGAERVVTLLLEIPG"
BASE COUNT         1229 a         1991 c         2025 g         1178 t
ORIGIN      
        1 acgcggttcc accgggacag cagcaaacca agatcaaccc caccgcaaag gcggtgggta
       61 gttgaaatag gtagcctaca ccccagccga tgaacggcat gagcgtgtac tgcaagacga
      121 ctccccaaac gatgggcgcc ggcgcgcgga acacacgaga aaagtcggag agctccaggg
      181 tgagccccat gcccagcatg atgatctgaa gaccaggcgt gatcaggcca tagtctagaa
      241 accagacgaa tccgcgcgga taaacgagcg cgacggcacc gcctagagtg acccagagag
      301 gaaagagccg aacgaaggca gccacgagca cgcggtctag cacgacgggc gcagcagggg
      361 tcatgcgaga cgcccatgcc cttccatgct aggcatcgca ggtcatgttt cgcccaccta
      421 ccgacgacgt ccgtatcgaa ggccttcgcc cgctgattcc gccggccatc ctgatggagg
      481 aaatccatcc aggcgaggaa gccatccagc atattgcgga ggcgcgccag cagatcagcg
      541 acatcgtgca tggcgaagac gatcgcctct tggtggtggt agggccttgc tccattcacg
      601 acccggtggc cgccctcgac tacgccaagc gcctcaaagc gattcgcgat cagcatgcgg
      661 acgatctttt catcgtcatg cgcacctatt tcgaaaagcc ccgaacgacc gtcggttgga
      721 agggactgat caacgatccc aatctcgacg ggagcttcga catcaatcac gggcttcggg
      781 tcgcgagagg gtttctcagc accttgggcg aaatggagct gcccgcagga acggagttcc
      841 tagacccgat ctcgccgcag ttcgtcgcgg acctggtctc ctggggagcg attggggcgc
      901 ggacgacgga aagccaggtg catcgcgagc tcgcaagcgg tttgtcgatg cccgtcggct
      961 tcaagaatgg aacgcgcggc acggtcgtca ttgcggtcga agcggttcgc gcggcccgtc
     1021 atcctcatca ttttctctcg gtcacgaagc aggggctcac ggcgatcgtc gcgaccaagg
     1081 gtaacctcga ttgccacgtc atcttgcggg gcggtgcggg caagccgaac tacgagcccg
     1141 agttcatttc gtccacagtc gagttgctcg agagcgccgg gctcgatggc agcgtcatgg
     1201 tcgactgcag ccacgccaac agcggcaagg accacgaacg gcaagccggc gtcgccatcg
     1261 cggtcggcca gcagatcgca cgaggcagca ctggggtttt cggtgtcatg ctcgaaagct
     1321 tcctcgtggg tggacggcag gatcaagagc ccggcgtgga gctcacctac ggccagagca
     1381 tcaccgacgc ctgcatgagc tgggacgcga ccgtgcccgt gatcgaaggc cttgccgatt
     1441 ccgtccgcaa gcgccgcaac gcaggcggct gagcccccgc gtcgccctcg gctctcagcg
     1501 aatttccggc tcaggcacgg gaacctggat gctgctcggc aacgtatcga aacgcgcggc
     1561 cttcacgatt ttctcgcgcg tgtcgcaggt ttcgaagcgc gttctcccag caagccattg
     1621 actcggacaa cagcgatccg tctcgataga ctcggtttgc tgagcgcatg gcgccagctg
     1681 cggccgggaa aacgcagcga tgccggctat ctgccccgcc acgccgttga acgtcacgcc
     1741 cacgctgaac gcgtcgcatt cggcaccggt tccgtcgtgc tctggaaact ggtgtatgtc
     1801 cgcaaactga ccgaagagtg tttcgataac ggtcgcattg atcgagcaca tgcctatctg
     1861 gggcgtagcc tctccaagct tctcgagact gaatcgccca gcgaacaggc cgtgctcaag
     1921 ccgggtgcgg tcgtcgtgga tgtcccccat gatgactccg ccgttcaccg tgacggtcat
     1981 ggcgccgtcg ctcgtgagcg taccgtcgcc cgcgatcagc ttgaactccg tgccgttcat
     2041 caggggcacc acgaggcggc cgtaggatac gtacgcatcc acctgctcgg ccttcggaag
     2101 ctctaggctg gcgcggcctg ttggacctgc gctgaaatcg atcgggtcga ggaaccaaga
     2161 gtccttgccg tcccaagcgg gctccggggc ctcgacatcc tggcttccgc tcaggtacac
     2221 cagattgagt ggatcgctat cggcgaatcc gacgagtgac ggatctgcgc tggtcccatc
     2281 gaccgccgtg gtgagcatcg ccgtaacgct cgcgtcgttt ggctgcccgt tccagtgtcg
     2341 gacccagagc aagacggtgc cgatgccccg tccttgggtg agagcgagat tgtcttcgag
     2401 gcatgggagt gcgcttccag tcgtcggaag caaggtcgtg ccaatcgcgt tgtcgattcc
     2461 cttgctgccg tcgaccacgg cctggcccac cacttcgcca tcgacggtcg gaggctgaca
     2521 ggtcgcatca tcgcgtcccg tggttgccgt ggcatccaag tcgatgccga tccgagccgc
     2581 tgtctccgcg gactgtcgga gaaagatgtc gttgagcccg aatacgagtg actctgcgtc
     2641 atcaggcgag ctcgtgctgg cgggaggccg attggggact cccgcactcc catcatcgat
     2701 caagctcgcg tcgtagacgt tgcagcctag cgcaaggaag aggcccagca ggccgcaagc
     2761 ccgacttggc acgcgcagaa cttcatcgct gcttccccac accgttttag agtagcaaga
     2821 gcgacgtcga ggccaaagaa cctaggcaga gggcgccatg gtgcgaatga acatctcaat
     2881 catctcccac ggcagattcg acggcaggtt gagaatggga aggtcgatac cgtattcccg
     2941 gcgacgctgg agctcggaaa tgcagtactg cggctcgccg gcgatggtca gcgcttcgat
     3001 catggcctcc ggaaccgcat ccgccgcctg ggagcgagtc ttcttgtcgc ggtagagctc
     3061 gtcgatcctc ttgcatgcgt cggcgtagcc gaatccgccg agcagctctt tgtagtagtc
     3121 gcccatgccc ccgatgtaga aggcgatgat ctgcctcgcc tcgcgagctc cggcgtcacc
     3181 gagcgggatc accgtggtga atggtgcggt tgtgatctcg ctcgggtcac gacctgcttt
     3241 ggcggcaccc ttggcgatcc aagcgtggcc tcgtttgagc tcggtgtacg gccagaacgt
     3301 ggggatccaa ccatcggcca gctcgccgat cgaagtgatg gaattctcct tgagtgcggc
     3361 tacgtagatc ggcacgtcgc tacgtaaagg cttcatcgcg agctcgaaag gccggtactc
     3421 gtgaagcttc gcgccactct cggtgagacg ctttccgctt agcagggtat tggtcacgcg
     3481 aatcacgtcc cgcaactgag tgagcggttt tttgaactca cgtgcgtgaa aaccctcgat
     3541 gacgcgttgg ccgctggtgc cgaggccgag aatgagacgg ccctcgctga tttcatcgac
     3601 ggtcgctgcg ttcatggcca agagaccggg tgaacggctg aagcagttga cgatcccggt
     3661 gccgagcttg attcgtttcg tgtgcacggc gatctccgtc agcaacgaaa agatctcgta
     3721 accccaggcc tccggaatcc agaatgaatc gtagcccagc tcgtcggcga gaatcgcggc
     3781 cctgagatag gtttttcggt cgtagttttt ccagaatggg agaagcccgg tacgtgcgct
     3841 catcgaaacc tccggatgcg ggagtagaat gtttggagcg cagtttcaag gacctgccac
     3901 gcttgacagc gtcctggaat tcgtgacacg agcctgactt tacggggagc ccgaacgaat
     3961 gaagatcgac gccggattgc ttgtcacgaa catgaaccag ataggcgtcc gggttcgtga
     4021 gctcgaggaa gccggcttcg acgggtgctt caccttcgaa gggccacacg agccattcat
     4081 gcccctcgtc ttggcagccg agcactcgaa gctggagatt gggacgggtg tcgcaatcgc
     4141 gttcgcgcgc acgcccatgc tcgttgcgaa cctcgcccat gatctgcagc actattcggg
     4201 cggccggttt tggctcgggc ttgggtcgca gatccggcct cacatcgagt cgcgattcag
     4261 catgccatgg gacaagccgg tctctcgcat gaaggagttc gtgcttgcgc tacgcgcgat
     4321 attcgcgagc tggaactcga atgagaggct cgattttcgc ggcgagttct acaagcacac
     4381 gttgatgccg ccgattttca acccggggcc ggccaccggt ggcgcaccgc cgatcttcct
     4441 aggcggcgtg ggtcctcgga tgacggaagc ggccggcgaa gtggcagacg gcatgcttct
     4501 gcacccgttc cacaccgagc gctccctcga gaaggtgatg ctgcccaatg tgcagcgcgg
     4561 gcttgcaacg tccgggcgcg cagcatcgga cttcgcgctc tgcgcccagg tgatggtcgt
     4621 gaccggagag aacgaagccg gctttgagaa cgcccgtgca atggcccgca accagatcgc
     4681 cttctatgcg tcgacgccgg cgtaccgaag tgtcctcgag atggagggca gagaagagct
     4741 gcagccggag cttcgtcagc tcaccaagga aggtcgctgg cacgaaatgg cggcgcgaat
     4801 cgacgacgag ctcctcggca agatcgcagc cgttgggaca ccgaaagagg tcgcgagcat
     4861 cctgcaccgt cgctacggcg gttttgccga aaggcttgcc ttctcttcgc cgttccccat
     4921 tccgcaggcc tgttcggccg agatcatcag cgcgcttaga agccacccga agcccgcatg
     4981 accaagcccc ggtgcattcc gctcgacgcg gtttccgatg agagggtcgg tggaaaggcc
     5041 gaaggactcg ttcgactact cggttatggc ttcgaggtgc cccctggttt cgtcattctg
     5101 ggcgcgacgc ctcagaacct tccggccgat ctggacgagc actacgcgcg gatcggcgga
     5161 ggaagagttg ccgttcgctc ctcggcgatc ggtgaagacg ccgaggacgc ctcgttcgcc
     5221 ggccagtacg agaccgtact gaacgtcgaa ggcacagagg gattgcgggc cgcaatcgag
     5281 gactgcctgc aatcgctcga aaatgcccgg gcggtcgcct accgcgacga gaaagccggc
     5341 ctcacggacg tgacgatgaa catcatcgtt cagcagatgg tcgacgcgcg caccgcaggg
     5401 gtcctgttca cagcgaaccc cgtcaacgcg cgtcgcgatc aagtcgtcgt cgatgcggtc
     5461 gcggggctgg gcgaggcgct ggtgagcggc cacgcgacac ccgaccattg gctgctgatg
     5521 cgagacgggt cggtcctgct gagcgaagtg cagggagaaa cgcccgtgct cacgactgca
     5581 gagctccagc tcttgctaga cggggccgtt cgcgccgaag agcgatacgg ggcgccgctc
     5641 gacatggaat gggccatcga tggcaagggt gccgttcgct ggcttcaagc tcgtccaatt
     5701 acgcaacttc ctgcagaccc gaacgagctc gacaccgatc cgaacccccg tgacgtctac
     5761 acctggtgca acatcggaga gatgatgcca ggcgctgtca cgcctctgac gtattcggtg
     5821 acggggcgtg gcatcgacat cggcatgcag cgtgcgtaca ggcgaatggg ggctgaggtg
     5881 ccgccaggcg aaggcgttcg ctacgtgggc atgttctatg gtcacctttt catcaacctc
     5941 tctacgcttg ctgagctttc ggccgcggtc gcgggttcga gcaagggcca gatgtgcatc
     6001 gcgctctgtg gacgcgacat cgaagaggtg agcgagccgc agccggcccc tgcatggagg
     6061 cgcgcgctga atggcgcccg ctactttgcc gttctcttcg gggccgcgaa gcatcgaaga
     6121 cgtctcgacg cgctcctcga cgatcttcgc cttcccaagc ttggcacctc tcatcagctc
     6181 taccgcgcca tcgatgaacg gcttccgctt gtttggaggg cctacgagct gcatctcatg
     6241 tcctcggcct cgtccggtgc cctcagccca atcctcctag gcatcctcgc caagggcggg
     6301 gagcccaccc aagaacacca cgccgaagtc gcggagatgc tcgccggcgc ggcagatgtc
     6361 gagagcgcgg acatcgccga gggcgccgag cgggtcgtga ccctgctcct cgagataccc
     6421 ggt
//
LOCUS       DASRZF010000101         7726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3512188, whole genome shotgun sequence.
ACCESSION   DASRZF010000101 DASRZF010000000
VERSION     DASRZF010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 7726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7726
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3512188"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1120
                     /locus_tag="VFG22_04505"
     CDS             <1..1120
                     /locus_tag="VFG22_04505"
                     /inference="COORDINATES: protein motif:HMM:NF017591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HET6415532.1"
                     /translation="TLSRCGGSDGGGATTSNIANLGPLQDPDENGLRLPIGFTSRVVA
                     RSGMPVGSSGYLWHNAPDGAAVFTTNDGGWIYVSNSETAAISGGGASAVRFSSNGDVV
                     DAYRIVGDTNHNCAGGATPWGTWLSCEETPTGLVWECDPLGERDAVARPAMGAFKHEA
                     AAVDEANRHVYLTEDVGDGRLYRFVYESPEDLGTGELQVAEVTGSGPEGAVEWRTVPD
                     PSGGTEETRYQVPSSTPFNGGEGIWNHQGRIYFTTKGDNRVWVYDTATSMLTILYDVA
                     TSSTPILSGVDNLTVTPTGDVVVAEDGGDMEIVAVAAEGSVVSLVQVTGQELSEVTGP
                     IFGPSLQRLYFSSQRGPDGRDILGRSGITYEVSGPFFI"
     gene            complement(1124..2293)
                     /locus_tag="VFG22_04510"
     CDS             complement(1124..2293)
                     /locus_tag="VFG22_04510"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HET6415533.1"
                     /translation="MKRIAFSATLLGLLMASSAHAHQKSVSYSKWTLLEQGGIAEVRV
                     RWLELTSLPSIQDGGDAPFEAASILPYLQSRLLLEADGRPCEAIPASGTWLPAERGWV
                     RVEWKVRCDSAPTGLHSELFTSLTNHVHLSTIEGPNPVDVVLSSTAPRARIDSSGAPG
                     AGVGFAPYLRLGVEHILSGWDHLAFLFLLIVVAQRIREVAVLVTGFTVGHSITLAAAA
                     LGVVVPHVRAVEATIAASILIIALENARIERLHGGLLVIGGALMLFVASAAFGGLPAF
                     WGIALFTACYFALLRMLTKPGRLRWLIACLFGFVHGLGFSGVLLEQELPRPQLVQALL
                     GFNLGVELGQLMVLACIWPLLAWMRARQRGGAVIDATSFAGVALGTFALLVRAFG"
     gene            complement(2290..3039)
                     /locus_tag="VFG22_04515"
     CDS             complement(2290..3039)
                     /locus_tag="VFG22_04515"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HET6415534.1"
                     /translation="MNRPWLLAVGMLVGAALAVYFIVRLPETREPTSDAVAWVSDRPI
                     SRASYENALQAVASDRKGGTLRADDRARVLERLIDQELLIDRAIELGLHERDPQIRNQ
                     LVTAMIDFLVRRAEDEAAAVSETDLRAFYQTEAFRFERRPQYRVAVEGAVVPLPGGWL
                     LDKEIEQRLGPSAVRKVAELEVGESADIGEGERRYTIRLLERQDGERVPFEEAREAVE
                     AAYARERSEAAVREFLELARKRTDVRIEVER"
     gene            complement(3036..5246)
                     /locus_tag="VFG22_04520"
     CDS             complement(3036..5246)
                     /locus_tag="VFG22_04520"
                     /inference="COORDINATES: protein motif:HMM:NF023649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HET6415535.1"
                     /translation="MAWWKKGCLTVVLGLVLLVLGFWLVYGGAKLQVDGEIARVPLAS
                     EHVAGREASQEHTAPKESDRILFGDLHVHTTLSVDAFMWSLPLMGGDGVHPPSDACDF
                     ARFCSQLDFYALTDHAEALNPRTWKMSRDSVRECNAVAAQSEQPDLLAFTGFEWSQVA
                     LTPEEHYGHKNVIFKYDSDDELPTRPISAPGMTTRAFSKLSAFWPLLEIPARSFPNQQ
                     GYLDFARHIRENADYPFCPDGVNSRQLPPTCRERAATPDILFQKLHELGLDALVIPHG
                     STWGFYTPLGYTWDKQLRRNMDDESLQRLVEVFSGHGNSEEYRSFRAANFTEEGMECP
                     EPTEDYEACCWRAGEIIRGRCEDPSSAQCQQRVEKARADFLRVSLAGHVTLPGEDVPD
                     WNNCGQCTDCYLPAYQYRPGGSVQYMLAKGDFEKPDEPRHTTMGFIASSDNHTARPGT
                     GYKEFARRKMTEARGAPSESWRRTMFGDRGNPEPESISYTLDDLMERPPFELMWMERQ
                     ASFFITGGLVAVHALERTREAIWDALQTRNVYGTSGDRILLWFDLKNGPEGALPMGSE
                     LPLSGTPKFEVRTAGSFEQKPGCAPDVIQALGEERVEQICAGECYNPGDRRRRITRIE
                     VIRIQRQQSADEPVDELIEDPWKTIPCPEGPELCVVEFEDPGYQALGRDTLYYVRAIQ
                     EPTPTVNGGGLRCKGEACEPCYGNFRTPADDDCLADAEERAWSSPIFLLAGSKP"
     gene            5349..6533
                     /locus_tag="VFG22_04525"
     CDS             5349..6533
                     /locus_tag="VFG22_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6415536.1"
                     /translation="MGSANMRDRGTQTGKRRVAHVTTVDLSLRYLLLNQLKRIQGEGY
                     EVFAISADGADVPMVEATGIRHFAVPMTRRITPLADLRCLWELVRIMRRERFDVVHTH
                     TPKAGLLGQLAARIAGVPIVANTLHGFYFHDDMKPVPRRFYIWMERVAAKCSDTILSQ
                     NREDMATAIAERIASPEQLKWLGNGIDVARFDRRSISESSLEDLRHEIDIDPNAPVIG
                     FVGRLVEEKGILDLLQAAGPVIQAIPTAQFLIVGPHDEEKPDALRPEVAERYGVAANC
                     RFVGMRNDMPELYALMDVLVLPSYREGFPRAPMEASAMGVPALVTDIRGCREAVQHGE
                     NGLRFPVGDSDALARALIELLGDETRRAEMGVAGRRMAEERFDEQKVFDRVLSEYERL
                     LA"
     gene            6530..7147
                     /locus_tag="VFG22_04530"
     CDS             6530..7147
                     /locus_tag="VFG22_04530"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET6415537.1"
                     /translation="MTFYRRTGKRLLDLSLTTPALLLLAPVGLALAALIRLKLGSPVL
                     FRQPRPGRDGQIFELIKFRTMTDARDPSGKPLPDDQRLTPFGGRLRRSSLDELPTLWN
                     VIRGDMSLVGPRPLLVQYLDRYTQEQSRRHEVAPGITGWAQINGRNAITWEEKFELDV
                     WYVENVSLRTDLAILFRTVVQVLAQRDISAEEHATMPEFMGSHEE"
     gene            7152..>7726
                     /locus_tag="VFG22_04535"
     CDS             7152..>7726
                     /locus_tag="VFG22_04535"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HET6415538.1"
                     /translation="MAIFGTGGFAREVACILHDLGRGDRIVAFHEPDDVYRRREILGV
                     AVRPQSAFDPTKHRAVVGIGNPAIRQKVVGELPAGTMFDTLIHPTAVMSQWVDVGPGS
                     IICAGCVLTCQISIGAHCHLNLNTTVGHDCTFGDFCTVAPGVNISGNCDFGMRVDVGT
                     QASFRQHIRVCDDAVIGMGTVVVRDIVQAGT"
BASE COUNT         1480 a         2431 c         2481 g         1334 t
ORIGIN      
        1 tacgctcagc cgctgcggcg gcagcgacgg aggcggggcg acgacgagca acatcgccaa
       61 cctcggcccg cttcaagatc ccgacgaaaa cgggctgcgc ctgcccatag gattcaccag
      121 ccgcgtagtc gcacgctccg gcatgccggt cggaagctcg ggctacctct ggcacaacgc
      181 acccgatggc gcagcggtgt tcaccacgaa cgacggcggc tggatctacg tctccaactc
      241 cgaaacggcg gctatctccg gaggcggcgc gagcgcggtt cggttctctt cgaatggtga
      301 cgtagtcgat gcttaccgaa tcgtaggcga caccaatcac aactgcgcag gcggcgcaac
      361 tccctggggc acctggctgt cctgcgaaga gacgcccacc ggcctggtct gggaatgcga
      421 ccccctcggc gaacgcgacg cggtcgctcg tccagcgatg ggagcgttca agcacgaggc
      481 cgccgcggtg gatgaagcga atcggcacgt gtacctcacg gaagacgtgg gggacggacg
      541 gctctaccga ttcgtctacg agagcccgga agacctcggc accggcgagc ttcaggtggc
      601 ggaggtcacc ggaagcgggc cagaaggcgc agtcgagtgg cggacggtcc ccgaccccag
      661 cggcggaacc gaagaaacac gctatcaggt cccgtcgagc acgccgttca acggcggcga
      721 ggggatatgg aatcatcagg ggcggatcta tttcaccacc aagggcgaca atcgggtctg
      781 ggtctacgac accgcaactt cgatgcttac gattctctac gacgtagcca cctcgagcac
      841 accgattctc agcggcgtcg acaacctcac agtgactccc acgggcgacg tggtcgtggc
      901 cgaagacggg ggcgacatgg aaatcgtcgc ggtcgccgcc gagggctcgg tggtgtcctt
      961 ggtgcaggtg acggggcaag agctttcgga agtcacggga ccgattttcg gtccgtcgct
     1021 tcagcggctc tatttcagct cccagcgcgg cccagatggg cgcgatatcc tcggcaggag
     1081 cggcatcacc tacgaggtga gcggtccgtt cttcatctaa gccctaaccg aaggcgcgga
     1141 cgagcagagc gaaggtcccg agcgcgacgc ctgcaaacga cgtagcatcg atgacggcac
     1201 cgcctcgctg gcgggctctc atccaggcga gaagcggcca gatgcaagcc agcaccatga
     1261 gttgccctag ctcgacgccc agattgaacc cgagtagcgc ttgcacgagt tgcgggcgcg
     1321 gaagctcttg ctcgaggagc acgcccgaga acccgaggcc gtggacgaag ccgaatagac
     1381 aggcaatgag ccagcgaagg cggcctggct tcgtcagcat gcgaaggagc gcgaaataac
     1441 aagccgtgaa taaggcgatt ccccagaacg cgggcagccc cccgaacgct gcgctcgcga
     1501 cgaagagcat cagcgctccc ccgatgacga ggaggccgcc atggagccgc tcgatgcgcg
     1561 cattctcgag cgcgatgatc agaatcgatg cggcgatggt cgcttctacc gcgcgcacgt
     1621 gcggaactac gacgccgagc gcggcggcgg ccaaggtgat gctgtgacct acggtgaagc
     1681 ctgtgacgag tacggccact tcgcgaatgc gctgcgcaac gacgatgagc aagaagagaa
     1741 aggcgaggtg atcccagccg ctcaaaatat gctcgacgcc gaggcgcaag tagggagcga
     1801 atccaacgcc agctccgggc gcgccgctcg aatcgatccg ggcgcgcggc gcggtggagg
     1861 aaagcacgac atcgacgggg tttggcccct cgatggtcga gaggtgaacg tggttcgtga
     1921 gggatgtgaa caactcgcta tgtaggccgg tcggcgccga gtcgcagcgg accttccatt
     1981 cgacgcggac ccatccacgc tccgcaggaa gccaggtgcc gctcgcggga atcgcctcgc
     2041 aagggcgtcc gtcggcttcg agcaagagcc gcgattgcag ataaggcagg atgcttgcag
     2101 cctcgaaagg agcgtccccg ccgtcttgga tcgatgggag cgaagtgagc tccaaccagc
     2161 gaacccgtac ttcggcgatg cccccctgct cgagcagagt ccacttcgag taggagacac
     2221 tcttttggtg ggcatgcgcg ctgctcgcca tcaggagccc gagcaaggtg gcggagaacg
     2281 cgatgcgttt caccgctcga cctcgatacg gacgtcggtg cgtttgcgcg cgagctcgag
     2341 aaactctcgc accgcggcct cgctgcgttc cctagcgtag gcagcttcga ccgcttcacg
     2401 cgcttcctcg aagggcaccc gctcgccgtc ttggcgttcg agcaaccgaa tcgtgtaccg
     2461 acgttcgccc tcaccgatgt cagccgattc tccgacctcg agctcggcca ccttccgcac
     2521 ggcgctcgga ccgaggcgct gctcgatttc cttgtcgagc agccagcccc ccggcagagg
     2581 caccacagcg ccctcgacgg caacccggta ctgggggcgg cgctcgaatc gaaaagcttc
     2641 ggtctggtag aatgcccgca ggtcggtttc gctcaccgca gccgcctcgt cttcggcgcg
     2701 ccggaccagg aaatcgatca ttgccgtcac cagctgattt cggatttgcg ggtctcgctc
     2761 atgaaggccg agctcgatcg cccggtcgat gagcaactct tgatcgatca agcgctcgag
     2821 gacccgcgcg cgatcgtcgg cgcgaagcgt accgcccttg cggtccgaag ctacggcctg
     2881 gagcgcattc tcgtatgaag cgcgcgaaat cggccgatcg ctgacccagg caaccgcatc
     2941 actcgtgggc tccctggtct cgggcaggcg cacgatgaag taaacggcca gggccgcgcc
     3001 gacgagcatt ccgacggcga gcagccaggg cctgttcatg gcttcgaacc ggccaagagg
     3061 aagatcggcg aggaccaggc ccgctcttcc gcatcggcca ggcagtcgtc gtcggcaggg
     3121 gtgcggaagt tgccgtagca aggctcacag gcctcccctt tgcaacgaag gccacccccg
     3181 ttgacggtgg gtgtcggctc ctgaatcgcg cggacgtagt agagcgtgtc tctgccgagc
     3241 gcctggtacc cggggtcctc gaactccacg acgcaaagct ccggcccctc ggggcatggg
     3301 atcgtcttcc atggatcctc gatgagctcg tccaccggct cgtccgcgct ctgctgccgc
     3361 tgaatgcgga tgacctcgat ccgggtgata cgccggcggc ggtcaccggg gttgtaacac
     3421 tccccggcgc agatctgttc gacgcgctct tcaccgaggg cttgaatcac gtcgggcgcg
     3481 cagcctggct tctgctcgaa cgagccggct gtgcgaacct cgaacttggg tgttccggaa
     3541 agcggcaact cgctgcccat gggaagtgcg ccctcggggc cgtttttgag atcgaaccag
     3601 agcaggatgc gatcgcccga ggttccgtaa acgttgcgag tctggagagc gtcccagatc
     3661 gcctcccgag ttcgctcgag ggcatgcact gccacgagcc cacccgtgat gaaaaaggac
     3721 gcctgccgct ccatccacat gagctcgaac ggaggacgct ccatcagatc gtcgagcgta
     3781 taggagatcg actccggctc gggattgcct cggtccccaa acatcgtccg acgccaggac
     3841 tcgctcggcg ctccacgcgc ttcggtcatc ttccggcggg caaactcctt gtacccagtg
     3901 cccgggcgag cggtgtgatt gtcgctcgag gcgatgaaac ccatcgtcgt gtgccggggt
     3961 tcatcgggtt tctcgaaatc gcccttggcc agcatgtact ggaccgagcc cccgggccgg
     4021 tactggtatg cgggcaggta gcaatcggtg cactgaccgc agttgttcca gtccgggacg
     4081 tcctcgccag gaagcgtcac gtgtccggcg agcgagacgc gaaggaagtc ggccctcgcc
     4141 ttctcgaccc gctgctgaca ctgagccgag ctcgggtcct cgcaccgccc gcgaattatt
     4201 tcgcccgcgc gccaacaaca ggcttcgtag tcctcggtcg gctccggaca ctccatgccc
     4261 tcctcggtga agttggccgc gcggaagcta cggtactcct cggagttccc atgtccggaa
     4321 aatacttcga ccaagcgctg caggctctcg tcgtccatgt tgcggcggag ctgcttgtcc
     4381 caggtgtacc cgaggggcgt gtagaaaccc caggtgcttc cgtgcggaat cacgagcgcg
     4441 tcgagcccca attcgtgaag cttctgaaac agtatgtccg gggtcgcggc gcgctcacga
     4501 caggtcggag gcagttgccg gctgttcacg ccatccggac agaacgggta gtcggcgttc
     4561 tcgcggatgt ggcgcgcgaa atcgaggtag ccttgctggt ttggaaacga tcgcgctggg
     4621 atctccagga gcggccagaa cgcgctcagc ttcgaaaagg cgcgcgtggt catgccgggc
     4681 gcagagatcg ggcgtgtagg caactcgtcg tcgctgtcgt acttgaagat gacgttcttg
     4741 tggccgtagt gttcctcggg cgtgagcgcg acttggctcc actcgaaccc ggtaaacgcg
     4801 agcagatcgg gctgctcgct ttgcgctgca accgcgttgc attcgcgcac cgaatcgcga
     4861 ctcatcttcc aggttcgtgg gttgagagcc tcggcgtggt cggtgagcgc gtagaaatcg
     4921 agttgcgagc agaagcgcgc gaagtcgcag gcgtcggaag gtggatgcac gccatcgccg
     4981 cccatcaaag gaaggctcca catgaaggcg tccaccgaaa gcgtagtgtg cacatggagg
     5041 tcgccgaaga ggatgcgatc ggactctttt ggagccgtgt gctcctggga cgcttcgcgc
     5101 ccggcgacat gctctgaggc gagcggaacg cgcgcgatct cgccgtcgac ctgcagcttg
     5161 gcgcctccat agacgagcca aaacccgagg acgagcagga ccaagccaag aacgaccgta
     5221 agacaaccct ttttccacca agccacgcgg gcggaagcat aggtcgggat cttcgggtct
     5281 ccaaacaatc aagctcgccg gcgtttgcct tgagggttca cgacagcacg tgctacggag
     5341 cttggaaggt ggggagcgca aatatgcgcg acaggggaac acaaacgggt aagcgtcgag
     5401 tggcgcacgt caccacggtc gacctttcct tgcggtacct gcttctcaac caactgaagc
     5461 ggatccaggg cgaaggctac gaagtcttcg caatcagcgc ggatggagcg gacgtaccca
     5521 tggtcgaagc gactggaata cgccatttcg ccgtgcccat gactcgccga attactccac
     5581 ttgcggatct gcgctgcctc tgggagttag tgcggatcat gcggcgcgag cggttcgatg
     5641 tggtgcatac gcacacgccc aaggctgggc tcctcgggca actggccgcc cggattgccg
     5701 gcgtgccgat cgtcgccaac acacttcacg ggttctattt ccacgacgac atgaagcccg
     5761 tcccgaggcg cttctacatt tggatggagc gcgttgcggc caagtgctcg gatacgatcc
     5821 tctcgcagaa tcgcgaagac atggcaacgg ccatcgccga acgcatcgcg agcccggaac
     5881 agctgaagtg gctcggtaac ggcatcgacg tagcgcgatt cgatcgtcga agcatatcgg
     5941 agagcagcct cgaagatctg cgacacgaaa tcgacatcga tccaaatgca ccggtcattg
     6001 gtttcgtggg ccgactggtc gaggaaaagg gcatcctgga cctcctgcag gccgctgggc
     6061 ccgtgatcca ggcaatcccc acggcgcagt tcctgatcgt gggaccgcac gacgaagaaa
     6121 agccggacgc gctgcgtccg gaggtcgccg aacgatatgg agtcgccgcc aactgccgct
     6181 tcgttgggat gcgtaacgac atgccggagc tctacgccct gatggacgtg ttggtgctcc
     6241 cgtcttatcg cgagggattt ccgcgtgccc cgatggaggc ctcggcgatg ggcgtgccgg
     6301 cgctggtgac ggacattcgc ggatgccggg aggcggttca gcacggcgaa aacgggctgc
     6361 gctttcccgt cggagactcc gacgccctcg cccgggcctt gatcgagctc ctgggagatg
     6421 aaacgcgacg cgcggaaatg ggcgtcgcgg gccgccgaat ggcggaggag cgcttcgacg
     6481 aacagaaggt gttcgaccgt gtcctcagcg aatacgaaag gctcctcgcg tgacgttcta
     6541 tcggcgcacc gggaagcgtc tgctcgacct cagcttgacc acgcctgcgc tgctcttgct
     6601 cgcgcccgtg ggtctcgcac ttgcggcgct cattcgcttg aagctcggca gccccgtcct
     6661 gtttcggcaa ccacggccag gtcgagacgg tcagatcttc gagctcatca agttccggac
     6721 catgacggac gcccgagacc cgagcggcaa acctcttccc gacgatcagc ggctgacacc
     6781 tttcggcggt cgcctgcgac gctcgagcct cgacgagctc cccacccttt ggaacgtcat
     6841 ccgcggagac atgagcctgg tcggtccgcg tcccttgctg gtgcagtacc tcgaccgata
     6901 cactcaggaa caaagccgcc gccacgaagt cgcccctgga atcaccggct gggcgcaaat
     6961 caacggtcgg aacgcgatca cctgggaaga gaagttcgag ctcgacgtct ggtacgtcga
     7021 aaacgtgtcg ctccgaaccg atctagccat acttttccga accgtggtgc aggtgttagc
     7081 ccaacgagac atttcagccg aggaacacgc cacgatgccg gagttcatgg gaagtcatga
     7141 agagtgatac cgtggcaatt ttcggaaccg gcggcttcgc gagagaggtt gcgtgcatct
     7201 tgcacgacct cggccgcggc gaccgaatcg ttgcctttca tgagcccgat gacgtctatc
     7261 gtcggcgcga gatcctcggt gtggcggtcc gaccacagtc cgcattcgac ccgaccaagc
     7321 atcgagcggt cgtcgggatt ggcaatcccg ccatcaggca gaaagtcgtc ggggagctgc
     7381 ctgccggcac catgttcgac accctgatac acccgacggc agtcatgtcg cagtgggtcg
     7441 acgtgggccc gggctcgatc atttgcgcag gctgcgttct gacgtgtcag atctcgatcg
     7501 gcgcgcactg ccatctgaac ttgaacacga ccgtcggaca cgactgcaca tttggtgatt
     7561 tctgcacggt agccccaggc gtcaacatca gcggcaactg cgacttcgga atgcgggtcg
     7621 atgtggggac gcaggcttcc tttcggcaac acatccgtgt ttgcgacgat gctgtgatcg
     7681 gaatgggcac cgtcgtcgtg agagacatcg tgcaggctgg caccta
//
LOCUS       DASRZF010000102         4548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152 k141_72795,
            whole genome shotgun sequence.
ACCESSION   DASRZF010000102 DASRZF010000000
VERSION     DASRZF010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72795"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="VFG22_04540"
     CDS             <1..676
                     /locus_tag="VFG22_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415539.1"
                     /translation="LLGAFFVLQAISAAHERGQPSRRTRAQLIGKLVKFALPLTFLLG
                     AAMWHNAARFDDPFEFGHRFLQVRWRSRIEIWGLFGTHYLPRNLTVFFLSLPWILESN
                     PFVRITQHGLAFWFTTPNLVWSLFPKKLDATIVALWAAVLPTAICTLLYQNTGWVQFG
                     YRFALDYLPFLFVLIALGGRRLGPLFLSCAALAVLINTFGAVTFDRYHQFYDDDPSQE
                     VIFQPD"
     gene            complement(664..1569)
                     /locus_tag="VFG22_04545"
     CDS             complement(664..1569)
                     /locus_tag="VFG22_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415540.1"
                     /translation="MALTLIVAMLLGLAAPFIRGWFWRVPFGLLSIATVLRSFIGAAL
                     TAIFIGVVALFALRATPMDQADINRIAGPLGGAVALLLLFNSARRMREVRGLSVLCQR
                     LREDDARSTAAPALDRLLDAARLRDERRYVALVLMATGPLIQAGMWTEARDRLRSLER
                     GALSESQTVLRNQALATCELQFDDVDAAQEAIDQIRRPTEGDIEVWLVAMEALLMAVR
                     GRSEEALSHLGRQDTEDNPSLRASHRLVHAHVLAAQGKTAAALEELESLRGEAGRAGL
                     ERVMLPCGPASPLAKKLLESADQSG"
     gene            complement(1573..2214)
                     /locus_tag="VFG22_04550"
     CDS             complement(1573..2214)
                     /locus_tag="VFG22_04550"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HET6415541.1"
                     /translation="MVLRLLTVMSVLGLVVGTSAPASADIYRCKRADGTQHYTNIREP
                     GRGCQLVVRGSKKSKHAAASKQKSNTSSIDRSKDPARYSRYNGLIDEAAQLYELPESF
                     IRAVMHVESNFNPTVVSRAGAMGLMQLMPQTARSMGVSDPFDVRQNILGGARYLRILA
                     NRFKGDLVLTVAAYNAGEGAVEKYDGIPPYKETQRYVRRVLKHYYAYRGEGAP"
     gene            2307..3257
                     /locus_tag="VFG22_04555"
     CDS             2307..3257
                     /locus_tag="VFG22_04555"
                     /inference="COORDINATES: protein motif:HMM:NF013686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415542.1"
                     /translation="MPTSTFFEGPEKKLAVVVVPGSPALRNLGDSAWRTVVEAAGAKV
                     LSVLRREQCDAYLLSESSLFVFDDWFLFITCGETALVNAIPEILRLVPEESIAFLMYE
                     RKSEHFPERQLTTFSEDARRLHAMLPGRALRLGEEHGRYIQLFHTTRPYAPRSKVPTL
                     EVLMYVIDEDVGAQFTHGRPARSKSTAAESGIEAILPGFETSEHAFEPSGYSCNALKG
                     EEYYTVHVTPGATGSYASFETNHDFRGRLGELVRPIVELFRPRAFDVVTFLPEPEPQL
                     PIPGYLLGDHVVDQLTGCQLSYFQYSVAPSGPRPARELEL"
     gene            3360..>4548
                     /gene="nadB"
                     /locus_tag="VFG22_04560"
     CDS             3360..>4548
                     /gene="nadB"
                     /locus_tag="VFG22_04560"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HET6415543.1"
                     /translation="MSFALEAARHSRVMVVTKRSQTDSATNWAQGGIAAVLDPTDSFE
                     SHVSDTMITGGGLSHRDVVEMVVRDGPSRIHELMNLGAKFSRSDEGRSLDLTREGGHS
                     ARRVVHAGDITGEEVQRVLVQAAVASPNIEIAEDHMAIDLVETSKFGGPRRVAGAYVL
                     DERTGEVKTCRARATVLATGGAGKVYLYTSNPDVATGDGVAMAYRAGAELANMEFFQF
                     HPTCLFHPEAKSFLISEALRGEGGVLRLRSGEAFMERHHDMKDLAPRDVVARAIDYEM
                     KLRGHDCVYLDMTHEPADFILERFPNIRARCLEYGIDMTKEPIPVVPAAHYMCGGVVV
                     DDEGCSTLPGLWAVGEVTCTGLHGANRLASNSLLEGLVYGHRTAIAARKALSLLPEVA
                     DEYV"
BASE COUNT          859 a         1424 c         1341 g          924 t
ORIGIN      
        1 gctcctcggt gcattcttcg tgcttcaagc gatctctgcc gcgcacgaaa gaggacaacc
       61 gagccggagg accagggctc agctcatcgg taaactagtc aagttcgcgc tgcccctgac
      121 ctttttgctc ggagcggcca tgtggcacaa cgccgcaaga ttcgatgacc ccttcgagtt
      181 cggtcaccga ttccttcagg ttcgctggcg ttcgaggatt gaaatctggg gtttgttcgg
      241 gacgcactac ctgccgagaa atctgacggt cttctttcta tccttgccct ggatcctcga
      301 atccaatccc ttcgtccgca tcacgcagca tggactggcg ttctggttca ccacgccaaa
      361 cctcgtctgg agcctcttcc cgaagaagct cgacgcgacg atcgtcgcgc tttgggcagc
      421 ggttctgccc accgccatct gcaccttgct ctatcagaac acgggatggg ttcagttcgg
      481 atatcggttc gcgctcgact acctaccgtt cctattcgtc ctcatcgcgc tcggcggacg
      541 acgtttgggc ccgctatttc tgagctgtgc cgctcttgca gttctgatca acacctttgg
      601 cgccgtcacc ttcgaccgct atcaccagtt ctatgacgat gatcccagtc aagaggtcat
      661 ctttcagcca gactgatcgg cgctctccag aagcttcttg gcgagaggac ttgccggacc
      721 acacggcagc atgactcgtt cgagccccgc gcgccccgcc tctccacgaa gtgattccag
      781 ctcctcgagt gccgcggccg tcttgccctg cgcggccagg acatgcgcat ggacgagacg
      841 gtgtgatgcc cggagcgaag ggttgtcctc ggtatcctgt cgtccgaggt gtgagagcgc
      901 ctcttcggat cgcccacgca cggccatcaa caaggcttcc atcgctacca accagacttc
      961 gatgtcgccc tcagtaggcc ggcggatctg gtctatcgct tcctgcgcag cgtccacatc
     1021 atcgaattga agctcgcacg ttgcgagcgc ttggttgcga agaaccgtct gcgactctga
     1081 cagcgcacct cgctcgaggc tcctcagacg atcgcgtgct tccgtccaca ttcccgcttg
     1141 aatcagaggt ccggtcgcca tcaagaccaa tgcgacgtac cgccgttcgt ctctcagacg
     1201 cgcagcatcg agaagccggt cgagtgcggg cgctgcagtc gaccgcgcgt cgtcttccct
     1261 caggcgctgg cagagcacgg aaagccctcg aacctcccgc atcctccggg ccgagttgaa
     1321 caacaagagc aatgcgaccg ccccgccgag cgggccggcg attcgattga tgtcggcctg
     1381 atccatcggc gtcgcacgaa gggcgaacag agcaacgacg ccgatgaata tcgccgtcag
     1441 tgccgctccg atgaacgagc gcagcactgt ggcgatcgac agcaagccga aaggcaccct
     1501 ccagaaccag ccgcggatga aaggcgccgc caatcccaac agcatcgcca cgatcagcgt
     1561 cagagccatg ggctacggtg cgccctcccc acggtaggcg tagtagtgct tgaggacgcg
     1621 gcgaacgtac cgctgtgttt ccttgtaggg aggaatgccg tcgtacttct cgacggcgcc
     1681 ttcgccggcg ttgtatgccg ccaccgtcaa gaccaaatcg cctttgaatc ggttggcgag
     1741 gattcgcaga tatcgcgcac cgccgagaat gttttgacgc acgtcgaatg gatcagagac
     1801 gcccatggac ctcgcggtct ggggcatgag ctgcatcagc cccatcgccc cggcgcggct
     1861 caccaccgta ggattgaaat tgctttcgac gtgcatgacc gcccggatga acgactcggg
     1921 aagctcgtac aactgcgccg cttcgtcgat gagtccgttg taacggctgt agcgagctgg
     1981 gtctttcgaa cggtcgatgc tcgaggtgtt cgatttctgc ttactcgctg ccgcatgctt
     2041 cgacttcttg ctgccgcgta ccaccagctg acaaccgcga cctggctccc taatgttcgt
     2101 gtaatgctgc gttccgtccg cgcgcttgca gcgatagatg tccgcgctcg ccggtgccga
     2161 ggtccccacc acgaggccca gcacgctcat caccgtcagc agtcgcaaca ccatcgaccc
     2221 gaagtgtatc tccgggtgcg aagtcgcgcc aaaagggagg actcacgctt gcgctcgcgc
     2281 ctcttctgcg catgatggga cctctgatgc cgacttctac tttcttcgaa ggccccgaga
     2341 aaaagctggc ggtcgtggtc gttccgggct ctccggcctt gcgcaatctg ggtgactcgg
     2401 cttggcgcac cgtggtcgag gcagcgggcg ccaaagtcct ttcggtgctc agaagggagc
     2461 aatgcgacgc ttacctcctc tccgaatcga gtctgttcgt cttcgacgat tggttcctct
     2521 tcatcacctg cggcgagacc gcgctagtca atgcgattcc ggagatcttg cgcctggttc
     2581 ccgaggagtc gatcgctttc ctcatgtacg agcggaagag cgaacatttt ccagagcggc
     2641 aactcacgac cttctccgaa gacgctcgtc gcttgcacgc gatgcttcca gggcgggcac
     2701 tccgtcttgg cgaggagcac gggcgctaca tccagttatt tcacaccacc cggccctacg
     2761 ctccacgaag caaggtgccg acgctggagg tgctcatgta tgtcatcgac gaggacgtcg
     2821 gcgcgcagtt cacccacggg cggccggccc gtagcaaatc aacagccgcg gaatctggga
     2881 tcgaggcgat tcttcctggt ttcgagacat cggaacacgc cttcgaacca tctggatatt
     2941 cctgcaacgc gttgaaaggg gaggagtact acactgtcca tgtcactcct ggcgcgaccg
     3001 gtagctacgc gagcttcgag acgaaccatg actttcgagg ccggcttggc gagttggtcc
     3061 ggccgattgt cgagctattc cggccgcgcg ctttcgacgt cgtcacattt ctcccagagc
     3121 cagagcctca gctgccgatc ccgggctatc tgctcgggga tcatgtcgtg gatcagctca
     3181 ctggctgcca gctcagctat ttccagtact ccgtcgcacc ttccggcccc cgaccggccc
     3241 gcgagctcga gctctaggcg ggaagaatcc tcgtgtcgtg gagccgctcg gcctggtaga
     3301 ctccggcgtt catgcgagcg gaatgcgact acctggtgtt gggtagtgga gtagctgggt
     3361 tgagcttcgc actagaagcc gcacggcaca gccgcgtgat ggtcgtcaca aaacgcagcc
     3421 aaaccgattc agcaaccaat tgggctcaag gcggtatcgc tgcggtgctc gatccgacgg
     3481 attcattcga atcgcacgtg agcgatacga tgattacagg gggcggtctt tcgcatcgcg
     3541 acgtcgtcga gatggtcgtt cgtgatggcc cgagccgaat tcacgagctc atgaatctcg
     3601 gagcgaagtt cagtcgcagc gacgaggggc gctcgctcga tctcaccaga gaggggggtc
     3661 atagcgctcg acgtgtcgtg catgcgggcg acataacagg tgaagaggtg caacgcgtgc
     3721 tggtacaggc tgcggtcgcc tcgcccaaca tcgagatcgc cgaggaccac atggcgatcg
     3781 acctcgtcga gacgtcgaag tttggagggc cgcgtcgcgt cgcaggggcc tacgttctag
     3841 acgagcgaac gggagaggtg aaaacctgcc gcgcacgcgc gactgtccta gcaaccggtg
     3901 gtgccgggaa ggtctatctc tacacatcca atccggacgt tgcgaccgga gatggggtgg
     3961 cgatggccta tcgtgccggc gccgagctag cgaacatgga attcttccag tttcacccga
     4021 cctgcctgtt tcatccagaa gccaagagtt tcttgatcag cgaggctctg cgcggagaag
     4081 gaggggtcct tcggctgcgg agcggtgagg cgttcatgga gcgccaccac gacatgaaag
     4141 acctcgcgcc acgcgacgtc gttgcccgcg cgatcgacta tgagatgaag ctcagaggac
     4201 acgactgcgt gtatctcgac atgacccatg agccagcgga tttcattctc gaaaggtttc
     4261 caaacatccg cgcgcggtgc ctagaatacg gcatcgacat gacgaaagaa ccgattcccg
     4321 tggtccccgc agcccactac atgtgcggag gcgtcgtcgt cgatgacgag ggctgctcga
     4381 cccttcccgg cctctgggcg gtaggcgagg tgacatgcac tggtctgcac ggcgcaaacc
     4441 ggttggcctc taattcactt cttgaagggc tcgtttacgg tcatcgcacc gccatcgctg
     4501 ctagaaaggc tttgagcttg ctgcccgaag tggccgacga atatgtgg
//
LOCUS       DASRZF010000103         8870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2140703, whole genome shotgun sequence.
ACCESSION   DASRZF010000103 DASRZF010000000
VERSION     DASRZF010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 8870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8870
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2140703"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1255
                     /locus_tag="VFG22_04565"
     CDS             <1..1255
                     /locus_tag="VFG22_04565"
                     /inference="COORDINATES: protein motif:HMM:NF021285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HET6415544.1"
                     /translation="KSSIVSRIAHESCARAPSLLAEGHWWEELDRDFARQPEAFELAV
                     ADRLRVGAAASFDTVLRTAGASMISSMALPLGYNPIELRKAVRDSELYAAIAESADPA
                     RFFTPPPTGVRVRARKGRWLPRFEPDDGICEAISFDSPFQPVNPRQHKPYLRHSANRV
                     AHARLLRHRGNERRPTIVAIHGFTADWYLINEWVFALPWFYRMGCNIALFTLPFHGPR
                     QTRFSPFSGHGYFAGGTSRLNEAVAQSVFDFRILFDWLERERGAEQIGVTGLSLGGFT
                     AAMLASVEDRLAFAIPNVPVVSLADLVLEWQPIGVLLRGALSTMGMELSDARRLVAAS
                     CPLSYPPVLPPERLMIIAGVGDRLAPPKHSRLLWDHWGRCRIHWFPGSHIIHLDRGAY
                     LTEMARFMVGRGFLPEGELPPVEKR"
     gene            1310..1822
                     /locus_tag="VFG22_04570"
     CDS             1310..1822
                     /locus_tag="VFG22_04570"
                     /inference="COORDINATES: protein motif:HMM:NF017090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="emopamil-binding family protein"
                     /protein_id="HET6415545.1"
                     /translation="MDRVIPLSRRKGDIVVLVFFWINILFITYIVDVEQIVLPDISGD
                     WEYPLWPPAFCVDIIHWYGRNFDPVLIARPAWWRMTIWIDALFFGPFYITAIYAWTKG
                     KNWIRIPSIIWAAVMMTNVTIILGEELMGAHATPAPLMVLALNAPWLLVPVYVIKRMY
                     NTETPFTQSL"
     gene            1823..2620
                     /locus_tag="VFG22_04575"
     CDS             1823..2620
                     /locus_tag="VFG22_04575"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6415546.1"
                     /translation="MSEFSKRYGPWAVVAGASEGIGASFSEKLAERGMHLVLVARRSG
                     PLEALADEIRRKHGVDVRVESLDLGSPDAVSGMAAATEGLDVGLMIYNAAYSPIGAFI
                     DIDVEEHFKAVQVNVHGPLRLSHHFGRRFVERGRGGIILMSSMSGFQGTAMVANYAAT
                     KAYDTILAEGLWRELGPRGVDVLACIAGATLTPNYESSTDRIPTSGLARPMNPDEVTE
                     QALSDLGQRPSAVSGARNGLASLLLSRLLSRRAAIAMVSKETERMYG"
     gene            complement(2622..4628)
                     /locus_tag="VFG22_04580"
     CDS             complement(2622..4628)
                     /locus_tag="VFG22_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415547.1"
                     /translation="MSIMDDASTMHAERPGFPVDPYRLRRALLGGKRLLIASALFGFI
                     TGFAWVKLVMHSGYETTAVLKYEGDLQVAGLPPTHDALVPAADAFAHQSVLRKIAEEI
                     GYEDSLDLLERQIVYNVAPHLGTVDVTVKGETGEESAEFARIVVDVFMSYHKERQSRR
                     FEAEIARMRKRIDSAEHELSVARRRYNEFRDQHGIADLSTEQQSMLKSAASLRADGEL
                     ATSEIRALEARIQSLEKFLASTPKTSFQSGRESPERTTHNRLRQELASAKATLSAEHP
                     RVQSLQQQVNELGAQLRSGGGSSLGGDGIVSINASYQIIEGQLRESQATLEALRERQK
                     GLMQMADKAQTRVESFSEIEGEASALLAEVKVNEGLISSLHQDEAALEDALRDPPSGF
                     VVLDPGGVPEFPVRNKMKIVVFGTIFVLSVGFALFLVLRREFHGLLLETPAEFAFWGR
                     GPVLAATPWPDDPRGLDELVAGLDDFVPHVRGSLLILGGSKDEARLAMELADRMNDDW
                     FPAHGVTSTPATPRSAPAAPRGPLQTPPPPAGPWPRGPLQTPPPAGPYPIGASGTQSV
                     ALARLPSVPPDDRARLAHRSATDLQLEAWDGPFEGQSLRRAARLATRVVVLVRAGAMS
                     AIRLNGIQSRVGRQDGIGYIVVGLPPELASLPDRAGNVDQFWRS"
     gene            complement(4641..6059)
                     /locus_tag="VFG22_04585"
     CDS             complement(4641..6059)
                     /locus_tag="VFG22_04585"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HET6415548.1"
                     /translation="MTSKKPTEWRGGADAEFAPLMRLVDFVLIYVALWLPTYARGEVW
                     DQESWTAATLAAVLFLVIGQSLRLYENTRGAPLRALLARAWGGWFVGVVPVLLFLLFL
                     SKRSDDYSRIIVSSWFVLVPFLISVWRTSTTLVLRELRARGYNTRDAAIVGMTELGEQ
                     LAAQIKSVPSLGLQVRGFYDDRADDRCHPIPDSLGQRAGTLSDLVDAARSGEVDLIYI
                     AFPLRAEPRINVLLRELSDTTASVHLAADFLAFNLLHARWGAIGEVPTVSLHETPFYG
                     VDGWLKRLEDIVLGTLILLITAIPMFIIGLGIKLSSPGPVLFRQRRYGLNGEVIHVLK
                     FRSMNVTEDGPQITQATQGDARITRFGAFLRRTNMDELPQFVNVLEGSMSIVGPRPHA
                     VAHNELYRRKIQGYMLRHKVKPGITGWAQVNGWRGETDTLEKMEKRIEHDLDYIRNWS
                     LLWDLQIIFMTVFGSQARRNAV"
     gene            6187..7161
                     /locus_tag="VFG22_04590"
     CDS             6187..7161
                     /locus_tag="VFG22_04590"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HET6415549.1"
                     /translation="MGCARNHYTQAPTTPQDDPAFSAPEPVVLKGLESDPPEALKLLA
                     GDVIKLTTVSAKTQIFNGLIVDELGMLHVPLAGDIKVGGMTLSQAETAVERGLRQYDR
                     FVRANLVITRLDGHWAVAVGAVTKPGRIRVRPGLRVADLVAEAGGPLMGQSQLMPTLL
                     GDMDLARLVRNGETLPVDLPLAVQGDPRHNINIRPGDQLYVPPVTGRLIMVLGEVNRQ
                     TPIAFRQGIRLTEALAHAGGLVMSRADRHDIRIIRGPLREPLVYTTNFKALTSGEATD
                     VELAPGDIVYVTKSWYASTADVLNSLSQVLSLANSFAILAVAGAIGGR"
     gene            7162..7812
                     /locus_tag="VFG22_04595"
     CDS             7162..7812
                     /locus_tag="VFG22_04595"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HET6415550.1"
                     /translation="MRRPLFNRLLGSHVETEEVDPHTVRAGLVKAEKEAIDGAAQTRI
                     EQFTAGRACSRIALGRLGVATSTPIARGEDRAPVWPPGFVGSISHTDTWCAAAVARAA
                     DVRSIGIDLEQATPLKEALWRRVCTPKERDWLHELSTPGLTGKILFSAKEAVYKCQYP
                     ITQQFLGFHAVEMELGDGTFRATFQQQVGEFKPGDVMSGRFLVEEGLVATACELLP"
     gene            7809..8207
                     /locus_tag="VFG22_04600"
     CDS             7809..8207
                     /locus_tag="VFG22_04600"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HET6415551.1"
                     /translation="MTAEALVVVIDVPRGSFVKRGDDRAVDFVSPIPCPFNYGHVPGT
                     LAEDGDALDAVVLGPRLRPGTRATVTPRARIDFVDAGRSDPKWVCASAPLSPFQRLQV
                     SGFFRGYAVAKRLINRFRGHRGPTRYIGWL"
     gene            8266..>8870
                     /locus_tag="VFG22_04605"
     CDS             8266..>8870
                     /locus_tag="VFG22_04605"
                     /inference="COORDINATES: protein motif:HMM:NF016242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidogalycan biosysnthesis protein"
                     /protein_id="HET6415552.1"
                     /translation="MSSLRSVDANAWDRLVGEDDPFVEHAFLSLLEQSGSVGRGSGWE
                     PAHVTAWRGPLLVGALPLYLKTHSYGEYIFDWGWADAASRLGVSYYPKLVAMVPVTPV
                     TGRRFLFAPDEEPAALVRRLIDGCLEVADSTRASSIHILFLSPEEQAWVDLDGRLMKR
                     LSFQFHWRNEGYESFDDFVAEFRSSMRKKLRKERRIAAEANL"
BASE COUNT         1599 a         2786 c         2747 g         1738 t
ORIGIN      
        1 caagtcctcc atcgtgtccc ggatcgcgca cgaatcctgt gctcgcgcgc catccttgct
       61 agccgagggg cattggtggg aggagctgga ccgcgatttt gcccgtcagc cagaggcctt
      121 cgagctggcg gtcgcggatc gcttgcgcgt gggggccgct gcgagcttcg ataccgtcct
      181 acgcaccgcg ggcgcatcca tgatctcgag catggccctt ccgctaggct acaacccgat
      241 cgagctacgc aaggcagtcc gtgattccga gctctacgcg gcgatcgcgg agtcggccga
      301 ccctgcccgt ttcttcactc ctccgccgac gggcgtgcgt gtgcgcgcgc gcaagggacg
      361 ctggcttccg cgcttcgagc ccgatgacgg tatctgcgaa gcgatcagtt tcgacagtcc
      421 gtttcagccc gtcaatccgc ggcagcacaa accgtacctg aggcactctg ccaaccgagt
      481 tgcgcacgct cgtctgcttc gtcaccgggg caacgagcgg cgtccgacca tcgtcgcgat
      541 tcacggattc accgcggact ggtacctcat caacgagtgg gtattcgcgc tgccctggtt
      601 ctaccggatg ggatgcaata tcgccctgtt cacactcccg tttcatggcc cgcgccaaac
      661 acgcttctcg ccgttctccg gccacggtta cttcgcggga ggcacttcgc gcctgaacga
      721 ggccgtggcg cagtccgtgt tcgatttcag aattctcttc gactggctgg aacgtgagcg
      781 cggcgccgaa cagatcggag tgaccggatt gagtctgggc ggattcaccg ctgccatgct
      841 cgccagtgtc gaggatcgat tggccttcgc gatccccaac gtgcccgtcg tgagcttggc
      901 cgacctggtt ctcgaatggc aacccatcgg cgtcctcctc cgtggcgcgc tttctacgat
      961 gggaatggag ctgtccgatg cgcgacgtct ggtcgcggcg agttgcccac tcagttatcc
     1021 gccagtcctt ccgcccgagc gattgatgat catcgccggc gttggcgacc gcctcgcgcc
     1081 gcctaagcac tcgagactgc tctgggacca ctggggccgt tgccgcatcc attggttccc
     1141 aggcagccac atcattcacc tcgatcgggg ggcgtatctc accgagatgg cgcgtttcat
     1201 ggtcgggcgc ggctttttgc ccgagggcga gcttcctccg gtggaaaagc ggtagatttt
     1261 tcgctcgcgc cgagtaacct cgctcggaat ttgtcgaacg agggtattca tggatcgcgt
     1321 cattcccctg tctcgccgca aaggcgacat cgttgttttg gtcttctttt ggatcaacat
     1381 cctgttcatc acgtacatcg tggacgtcga gcagatcgta ctgcccgaca tcagcggtga
     1441 ttgggagtac ccactctggc ctccggcgtt ctgcgtggac atcatccact ggtacggccg
     1501 caacttcgat cccgtgttga tcgcccgccc ggcctggtgg cgcatgacca tttggatcga
     1561 cgcgctcttc ttcggtccgt tctacatcac tgccatctac gcttggacaa agggcaagaa
     1621 ttggatccgg attccaagca tcatctgggc cgcggtaatg atgacgaacg tcacgatcat
     1681 tctcggcgag gagctcatgg gcgcgcacgc gaccccggcg cccctgatgg tcctggcgct
     1741 caacgcgcct tggttgctcg ttccggtgta tgtgatcaag cgcatgtaca ataccgagac
     1801 gccgttcacc cagagcctct gaatgtctga gttctcgaaa cggtacggtc cctgggctgt
     1861 ggttgccggc gcgtctgaag gaatcggcgc gtcgttctcg gagaaactag ccgagcgagg
     1921 gatgcatctg gttctcgtgg cgcgtcgatc aggtcccctt gaggccttgg ctgatgagat
     1981 tcgccggaaa cacggcgtgg atgtgagagt cgaatcactc gacctcggat ctcccgacgc
     2041 cgtctccggc atggctgcgg cgacggaggg actcgatgtc ggcctgatga tctacaacgc
     2101 ggcgtactcc ccgattggag cgttcatcga catcgacgtc gaagagcatt tcaaggccgt
     2161 ccaggtcaac gtgcatggtc cgcttcgcct ctctcaccac ttcggccgcc gcttcgtcga
     2221 gcgtggccgg ggcggaatca tcctgatgtc gtccatgagc ggttttcaag gcacggcgat
     2281 ggtcgcgaac tacgcggcta ccaaagcgta cgacaccatt ctggcggaag gactctggcg
     2341 tgagctgggt ccgcgcgggg tcgacgtact ggcctgcatc gcgggggcca ccctcacgcc
     2401 caactacgag tcgtccacgg atcgaatccc caccagcggc cttgccaggc ctatgaatcc
     2461 ggacgaagtc accgagcaag cgcttagcga tctcggacag cgcccaagcg ccgtgtccgg
     2521 cgcccgaaac gggcttgctt cgctgctcct cagtcggctc ctgtcacgac gagcggccat
     2581 tgccatggtc agcaaagaga ccgaacgcat gtacggctga gctaggatct ccagaactgg
     2641 tcgacgtttc ccgcacggtc gggaagcgat gccagctcgg gcggcagacc gactacgatg
     2701 tatccgatgc cgtcttgacg acccactcgg ctctgaattc cgttgagccg aatggccgac
     2761 atcgctccgg cgcgaactag gacgacgacg cgggtcgcaa gtcgcgctgc ccgccggagc
     2821 gactgcccct cgaacggccc gtcccaggcc tcgagctgca agtcggtcgc cgaccggtgc
     2881 gcgagtcgtg cgcggtcgtc aggaggcacc gaggggagtc tggccagagc caccgactgc
     2941 gtacccgaag ccccgatagg atagggtccc gctggcggtg gagtctgcag cggacctcgc
     3001 ggccagggtc cggccggcgg tggtggagtc tgcagtggac ctcgcggcgc cgctggggcg
     3061 cttcttggag tcgccggtgt gctcgtgact ccgtgggcag gaaaccaatc gtcgttcatc
     3121 cgatcagcga gctccatggc gagtcgggcc tcgtctttgg aacctccgag aatcaagaga
     3181 cttccccgga catgagggac gaaatcgtcc agccccgcga ccaactcatc caagcctcgg
     3241 ggatcgtcgg gccacggcgt tgcagccagc acgggtcctc tgccccagaa ggcgaactcg
     3301 gccggagtct ccaagagaag gccgtggaac tcccgccgca agacgagaaa cagcgcgaag
     3361 ccgacgctca ggacgaagat ggtcccgaag accacgatct tcatcttgtt ccgcaccgga
     3421 aattccggga ctccgcccgg gtccagaacg acgaatccgc tcggcgggtc gcggagagca
     3481 tcttcgagcg ctgcctcgtc ttgatgcagg ctgctgatga ggccctcgtt cacctttacc
     3541 tcggcaagca gggcggaagc ttcaccctcg atctccgaga aggactcgac tcgcgtctgt
     3601 gctttgtcgg ccatctgcat gaggcctttt tgccgttctc gaagtgcctc cagcgtcgct
     3661 tgcgattcgc ggagctgccc ctcgatgatc tgataggaag cattgatcga gactatgccg
     3721 tcgccgccca ggctcgatcc cccgcctgat cgaagctggg cgcccaactc gttgacctgc
     3781 tgctgcagag actgcacgcg cggatgctcc gcggagaggg tggctttcgc gctcgcgagc
     3841 tcctgacgca agcggttgtg tgtagtgcgc tcaggcgatt cgcgtcccga ctgaaagctc
     3901 gtctttggag tactcgcgag aaacttctcc agactctgaa tccgggcttc gagcgcacga
     3961 atctcggaag tcgcaagctc gccgtcggcc cggaggctgg ccgcggactt cagcatcgat
     4021 tgttgctcgg tggagagatc agcgatcccg tgttggtccc ggaactcgtt gtatcggcgc
     4081 cgcgcaacgc tcaattcgtg ttcggcggaa tcgatccgct tccgcatgcg cgcgatctcc
     4141 gcctcgaatc gtctcgattg gcgctctttg tgataactca tgaagacgtc gacgacgatt
     4201 cgcgcgaact ccgccgactc ctcgccggtc tcgcccttca cggtaacgtc caccgtgccc
     4261 aaatgggggg cgacattgta gacgatctgc cgctccagta agtccagact gtcctcgtag
     4321 ccgatctctt cggcgatttt tcgtagaacc gactggtgcg caaacgcgtc ggcagcaggc
     4381 accaaagcat catgggtcgg cggaagaccc gccacttgaa ggtcgccctc gtacttgagg
     4441 acggccgtcg tttcgtatcc gctgtgcatc accaacttga cccaggcaaa gccggtgatg
     4501 aagccgaaga gcgccgaggc gatcagcaat cgcttcccgc caagcagcgc acgccgcaga
     4561 cgatatggat cgacggggaa gcctggacgt tcggcgtgca tcgtgcttgc gtcatccatg
     4621 atcgacatcg gctcctccgg tcaaaccgcg ttccgccgtg cttgggaacc gaacaccgtc
     4681 atgaagatga tctgcaagtc ccacagaaga ctccagttcc gtatatagtc gagatcgtgc
     4741 tcgatgcgtt tttccatctt ttcgagggtg tcggtctcgc cccgccagcc gttgacttgg
     4801 gcccagcccg taattccggg tttgaccttg tgtcgtagca tatagccctg gatctttcgg
     4861 cggtagagct cgttgtgcgc aaccgcatgt ggtcgggggc ccacgatgct catggagcct
     4921 tccagcacgt tcacgaactg gggcagctcg tccatgttcg tgcgccgcag aaacgcgcca
     4981 aacctggtga tgcgtgcgtc gccctgcgta gcctgcgtga tttgaggtcc gtcctcggtg
     5041 acgttcatcg agcggaactt caagacgtga atgacctcgc cgttcaagcc gtaccggcgc
     5101 tgtcggaaga gcacgggacc tggggagctc agtttgatgc ccaggccgat gatgaacatc
     5161 ggaatcgcag tgatgagcag aattagcgtt ccgagcacga tgtcctcgag gcgcttgagc
     5221 caaccgtcga cgccgtagaa tggtgtctcg tgcaggctca cggtcgggac ctcgccgatc
     5281 gcgccccatc gcgcatgcaa gaggttgaaa gccaggaagt cggctgcgag atgtacggag
     5341 gcggttgtgt ccgacagctc gcgcaagagc acgttgattc gcggttcggc gcgcagtgga
     5401 aacgcgatgt agatcaggtc gacctcgcca gacctcgccg cgtcgaccag gtccgacaag
     5461 gtcccggcgc gttgcccgag tgagtccgga atagggtgac agcggtcgtc cgctcgatcg
     5521 tcgtagaatc ctcgaacttg gagcccgagt gatggcaccg acttgatctg cgctgcgagc
     5581 tgctctccaa gctcggtcat tcctacgatt gccgcatcac gcgtattgta accgcgggcc
     5641 cgaagctcgc ggagcaccag tgtggtgctc gtccgccaaa cggaaatcag gaacgggacg
     5701 agcacgaacc aggacgagac gatgatgcgg gagtagtcgt cggagcgctt cgagaggaaa
     5761 agcaggaata gtaagactgg aacgacgccc acgaaccagc cgccccaggc acgcgccaag
     5821 agcgcccgta ggggcgcgcc acgcgtgttt tcgtacaagc gaagtgattg ccctatcacc
     5881 aagaaaagca cggcggcaag cgtagccgcc gtccaggact cctggtccca gacctctcca
     5941 cgggcgtacg tcgggagcca gagcgccacg taaatcaaga caaaatcgac gagacgcatc
     6001 agcggagcga actcggcatc ggcgccgcct cgccattcgg tgggtttctt gctcgtcatc
     6061 gggcgatggg gaacagtata ggcatacgag acgcgtttct cacttctacc ttgcggaccg
     6121 ccgtttggaa cagtaaccac gactatgcgc cggtgcacga cattgttgct ttcgatcacg
     6181 ctcgctatgg ggtgcgcgcg gaaccactac acgcaggctc cgaccacacc gcaagatgac
     6241 ccggcgtttt cggcgccaga gccagtggtt ctcaagggcc tcgaatccga tcctccggag
     6301 gcactgaagc tgctagcagg tgatgtgatc aagctcacaa ccgtttccgc gaaaacccag
     6361 attttcaatg ggctcatcgt cgacgaactg ggcatgctcc acgttccctt ggccggcgac
     6421 atcaaggtcg ggggcatgac gctatcccag gccgagactg cagtcgagag agggctccgg
     6481 caatacgatc gcttcgttag ggcaaatctg gtcatcacga ggctggacgg tcattgggcg
     6541 gtcgcggtcg gagcggttac gaaaccgggc cgcatccgag tcagacctgg cctgcgggtc
     6601 gcggatctgg ttgcggaggc aggaggcccc ctgatgggtc agtcgcaatt gatgccgacc
     6661 ctgttgggtg acatggacct cgcgcgcctc gttcgaaacg gcgagacgtt gcccgtggat
     6721 ctgccattgg cggttcaagg tgacccgagg cacaacatca acatccgtcc tggcgatcag
     6781 ctttatgtcc ccccggtcac ggggagactc atcatggtct tgggcgaagt caacaggcag
     6841 acccccattg cattccgcca ggggatccgc ctcaccgagg cgctcgcgca tgcgggcgga
     6901 ctcgtgatgt ctcgcgccga tcgacatgac atccgaatca ttcgtggccc gcttcgagaa
     6961 ccactggtct acacgaccaa tttcaaggca ttgaccagcg gtgaggccac tgacgtcgag
     7021 ctcgccccgg gcgacatcgt ctatgtgacc aagagctggt acgcgagcac tgccgacgtg
     7081 ctcaattcgc tgtcccaagt gctctcactt gcgaactcct ttgccatcct ggcagtcgct
     7141 ggcgccatcg gcggccgcta gatgcgccgc ccactcttca atcggttgct cgggtcgcat
     7201 gtggagaccg aggaggtgga ccctcacacg gtccgagctg ggctcgtgaa ggccgagaaa
     7261 gaagcgatcg atggcgcggc ccagacgcgc atcgagcagt tcaccgccgg gcgagcgtgt
     7321 agccggatcg ctctaggccg cctgggtgtc gccacgtcca cgcccatcgc ccgaggagag
     7381 gatcgggcac ccgtttggcc gcctggtttc gtcggcagca tttcgcacac cgacacctgg
     7441 tgcgcggccg ccgttgcgcg cgctgcggac gtccgctcga tcgggatcga cttggagcag
     7501 gcgacgccgc tgaaggaagc gctctggcga cgggtttgca ctccgaaaga acgcgactgg
     7561 ttgcacgagc tctccacgcc cggtctcacc ggcaagatcc tcttcagcgc caaagaggcg
     7621 gtctacaagt gccagtatcc gatcacccaa cagtttctcg ggtttcacgc agtcgaaatg
     7681 gaacttggcg atgggacgtt tcgcgcgacc ttccaacagc aagtgggaga gttcaaaccg
     7741 ggcgatgtga tgtctgggcg attcctggtg gaggaggggc tcgtggcgac cgcctgcgag
     7801 ctcctgccat gaccgcggag gcgctcgtag tcgtcatcga cgttcccagg ggaagcttcg
     7861 tcaagcgcgg cgacgatagg gcggtggact tcgtttcgcc catcccttgc ccattcaact
     7921 atggccacgt gccgggaaca ctggccgagg acggagacgc actcgatgcg gtcgtgctcg
     7981 gtccacgatt gcgtccgggg acccgcgcga cggtgactcc gcgagcgcgc atcgacttcg
     8041 tcgacgccgg gcgttcggat cccaagtggg tctgcgcgtc cgcaccactg agccctttcc
     8101 agcggctcca ggtctcgggc ttctttcgcg gctacgcggt cgccaaacgc ttgatcaata
     8161 ggtttcgcgg gcatcggggg cccacccgct acatcggctg gctctagccc ctgccctcgt
     8221 gcacacttcc ccactcgctt ggctgagtcg atcgacataa ggatcttgtc gtcactacgg
     8281 tctgtagacg cgaacgcctg ggatcgactg gttggtgaag atgacccatt cgtcgaacac
     8341 gccttcctgt cgctcctgga gcagagcggc tcagtcggcc ggggctcggg ttgggagccc
     8401 gcgcacgtca cggcatggcg aggtccgctt ctggtgggcg ccttgcctct ctacctcaag
     8461 acacacagct acggcgagta catcttcgac tggggctggg cggacgctgc gtctcgtctc
     8521 ggggtgagtt actacccaaa gctggtcgcg atggtgccgg tcacacctgt gacggggcgc
     8581 aggttcctct tcgcccccga tgaagagccc gctgcgctgg tgcgcaggct gatcgacggt
     8641 tgtctcgagg ttgcggattc gacgagagct tcgtcgattc acatcctctt cctgagtccg
     8701 gaggagcagg cctgggtcga tctcgatggc cggctcatga aacgcttgtc gtttcagttt
     8761 cactggagaa acgaaggata cgaatcgttc gacgatttcg tcgccgaatt ccgctcgtcc
     8821 atgcgcaaga agctgcggaa agagcgtcgc atcgccgccg aagccaatct
//
LOCUS       DASRZF010000104         9772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3513928, whole genome shotgun sequence.
ACCESSION   DASRZF010000104 DASRZF010000000
VERSION     DASRZF010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 9772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9772
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3513928"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="VFG22_04610"
     CDS             complement(<1..785)
                     /locus_tag="VFG22_04610"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET6415553.1"
                     /translation="MTQVSSHPPFVVTGASGYAASWVVHELLSRGATVRATVRDPSNQ
                     DKCHHLLDMAAGLPGTLELVKADLLEPGSFDRAVRGAGAVIHTASPFLIGKIKDANAQ
                     LIAPAVQGTRNVLESVNKADSVSKVVLTSSVAAIYGDACDLADAPGDRFTEDDWNKTS
                     TAEHQPYNRSKTLAEKAAWDMQRAQSRWKLATINPGFILGPSKTPRADSESIKLMADM
                     IRGVYKFGAPPLCFGIVDVREVAKAHVEAAIRPEAEGRHILVA"
     gene            complement(820..1758)
                     /locus_tag="VFG22_04615"
     CDS             complement(820..1758)
                     /locus_tag="VFG22_04615"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HET6415554.1"
                     /translation="MNRISRVVLSLMTVLAGCDSSGESTESSQASLGCTDGMLDSSVT
                     HIDLGIEGLIRSYELHVPASYDGSQAFPLVMNFHGYTSNGTQQRGYTKMDETADAHDF
                     IVAYPNGLDSSWNAGLCCGESAMSGVDDVGFTRAILDDLGARGCIDLSRVYATGMSNG
                     GFMSHRLACEVSDRIAAIAPVSGVLGLEPSDCSPSRPVPMIQFHGTDDFLVPFEGGGL
                     AGSMPVSDSMDFWAEQNGCIDEPEVTFEQGIVTCETRDACTDGASVTLCTVEGGGHCW
                     PGEPCARIEGVNLGTATTDINANDAMWALFSTVSLQ"
     gene            complement(1755..2936)
                     /locus_tag="VFG22_04620"
     CDS             complement(1755..2936)
                     /locus_tag="VFG22_04620"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET6415555.1"
                     /translation="MERDKQIELLSECLELVEGKKPFMSDEETLIPVAEYIDEARFEQ
                     ERELLFRRSMNVLAHSSQIATPGDFITRDLVGAPVILVRDKDGRARAFLNVCRHRGAT
                     VELRERGNCRRFVCPYHAWTYETDGSLAAVRQQEGFPTLDLESTSLVALSCFEAAGLV
                     WVCPDPSVPVRAPDEATRRLIVELEGFGTSEAAVFASEARTWNANWKLIVDGGLESYH
                     FRIAHRNTIGSFFTDNTSTFECIGDHVRSVLPRVSILELRNQPESDWNIREHTHLLYA
                     ISPNASILMQERHFELILTTPISVDQTRIEIMTIAPKPGGGGYSEKAQAFLAANHAFT
                     QKTLDEDFVIAEQIQRGMPSGANEFFRFARFEAALSQWHRRLDERLARSALPRVRMPV
                     S"
     gene            complement(3053..3781)
                     /locus_tag="VFG22_04625"
     CDS             complement(3053..3781)
                     /locus_tag="VFG22_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415556.1"
                     /translation="MPIAARVSALLIIALLSVTATSACSEGTGSSSVTLTGQITEFEP
                     DSQGPGVPIADVEICQFGSFNCAFTDENGEYGLWLLQNRNVELSIVKEGFGSVLIARF
                     SGTKDLVGDAIMASDAVLSELAGEIGTAYPPDGTGSLTVTTFQGSVSDGNSLAGVSYA
                     LFGSNGRSFYVNDSGVPETSLTETQTSGTGGFVELAPVVVTLQLSGAGVNCTSDNSWS
                     AAASNAFRLPIRVGFTTQCAVSCE"
     gene            complement(3882..4742)
                     /locus_tag="VFG22_04630"
     CDS             complement(3882..4742)
                     /locus_tag="VFG22_04630"
                     /inference="COORDINATES: protein motif:HMM:NF018439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1207 domain-containing protein"
                     /protein_id="HET6415557.1"
                     /translation="MTNAKLGRLVFLAALVTPTLSTASAESAEDTQVRVFPRVSLFAP
                     LAADLRWPKFTLGYNYYFGDSDLTHAGSAEAGASIPIVQKKNSRRPGAWEIGFQGGIF
                     AVFDLAAPSSDLINADYLGGITFSYAFDSLVLFARYMHQSSHLGDEFLLNNEVDRINL
                     SFEEISVLLSYDPLAWARVYGGAAVIVRSDPSNLDRWSLQFGSEFRPVTAKTKRRLQL
                     LVAVNLQCWQESDWIPDASLVAGLTLDPIGESSYRVDFLLRYYIGRSPNGQFFTERIQ
                     TLGPAIQLYF"
     gene            complement(4793..5461)
                     /locus_tag="VFG22_04635"
     CDS             complement(4793..5461)
                     /locus_tag="VFG22_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415558.1"
                     /translation="MTDLSPLDAPWALGQRAYAQVLRHLGAMQAPTIVEFGSGASTAA
                     LARDLPDATILSVDNDATYFERTRAQLPPNARVELVYRPLVWQRHGGAPYLSYASCDL
                     PDAVDALLVDGPPHWTRRGREACLYQAMPSLKIGARIFLDDYRRAPERRIVRNWLSAY
                     PGAVSLVEVVEEGDHVAVLQKTAEAALPSPSFSRWADAHWQAFIQPLTSRVRTVSMRL
                     QGKN"
     gene            complement(5495..6481)
                     /locus_tag="VFG22_04640"
     CDS             complement(5495..6481)
                     /locus_tag="VFG22_04640"
                     /inference="COORDINATES: protein motif:HMM:NF024918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HET6415559.1"
                     /translation="MRIAYFVHGNGRGHAMRARSLVPALRARGHDVHLHSGEDALAML
                     REIGSVEIDTFDPGPRLARRFAARFNADRRLLRELQPDLVITDGDAPSLHAAALSRIP
                     SIAIGHGLLFAHCRLPIAIPLHARMHAALNAASASWVANRIIVVHFGELQPFDPRTVI
                     ARPDPRPDLFEGDGRRGDALLVYAGQADLSAYVRALHQRGHRLQVFGRAEHLPEGVRV
                     EPPDVQRFAEALRSCRGVVATAGSNLIGESIALGRPMLLLPPAQMIEQELNARLAERD
                     GFAIAASAEELDIPAIKRFETLLVRGVPPFQPGTPIVTEALLTCIAELVPAG"
     gene            complement(6568..7578)
                     /locus_tag="VFG22_04645"
     CDS             complement(6568..7578)
                     /locus_tag="VFG22_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415560.1"
                     /translation="MGEQSSGDPSGVDPGDRFGDRLSEIRRFGEGIRDAVPPRVRKGL
                     RRALSVVFFAVIGFVLVRQLSGTDWPEVLRAVPRSPWFYVLFVARFFLLPVTDALSYS
                     AVWATNLVRHFGAFLMKRLLNSSFSAGSGDVYLLLWSVNTLRVSYRKAFSAAKDVTLL
                     SAAAANTIAVLFLGAYLAYGDLSLMESVRPGVLGLIIGVTLATAVLSLLVIRFRGTVL
                     AVDTPVMCRIFGYHGVRSILRIVLLGLQWTVGLPGSSFSDWISLLVVDLLVSRTPFVP
                     AREFLFLSLALGLAGTIDAPEAQVTALFLTDTALLQVGALGSLIAAMVWRSKPHPLPT
                     ID"
     gene            7825..9636
                     /locus_tag="VFG22_04650"
     CDS             7825..9636
                     /locus_tag="VFG22_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415561.1"
                     /translation="MTSTFELQTVDLPSDARPAYTPGSPGVTVTNPKLITQFGRADFS
                     LNNARYTRYYLSDQADTQPDAIVVLVPGFEGGAATFAVLAESLARRAQRESSMVVEVW
                     AVDRRSNQLEDTAGLDLAEQEQDPSLGLDFLFGEELGLEMNETLASELNRRAVFYNTG
                     EDLAFMAQWTTLVHSQDIDAVVEEARSTARNANVFLGGHSAGTGFTARYAATDFNLEG
                     GDPEPGYAKLRGLVMLEGGGGSLPSESPSEDELDRIEARFDGGLYEAVQSQEPRCMDG
                     VTACTVETEAEDCDGLPNTSCKPVDAYSVVAGLLSPQLLAVSEVAALEGDLQGDTGIS
                     LMQADQNGIEGNNAVSKVPELAALTLLLGTAPATSVALIGLFLDDDGAAAATASFVAT
                     SLGTIGETVDGVRTWLNYGDPMPASVLTDNGPAPTELADAGRWGVEVESTDLEGHMIP
                     SFYQGQTNFSDWYYPSSGLGVVSGLGLDTTPLSAPPPEGRGRSDIENITQARNVNIPV
                     IAFGGSNGLVPVPGRMLAYADSLAACAAPSCDGTTARVVDREQPSSAFPTFGDAAGGF
                     EVFMSEGYSHVDVVSAEDDETNNVVAPLLAFIERNLE"
BASE COUNT         1880 a         2986 c         3241 g         1665 t
ORIGIN      
        1 tcggcgacga ggatgtggcg cccttccgcc tcggggcgaa tcgccgcttc gacgtgcgcc
       61 ttcgcgacct cacgaacgtc cacgataccg aagcagagcg gcggcgcgcc gaacttgtag
      121 accccgcgga tcatgtccgc catgagcttg atgctctcgg aatcggcgcg gggcgtcttg
      181 ctcgggccga ggatgaagcc cgggttgatg gtagctagct tccagcgact ttgcgcgcgc
      241 tgcatgtccc aggcggcctt ctcggccaga gtcttggagc ggttgtaggg ctggtgctcg
      301 gcagtgctgg tcttgttcca gtcgtcctcg gtgaaccggt ctccgggcgc gtccgcgagg
      361 tcgcaggcat cgccgtaaat ggcggccaca cttgatgtga gcacgacctt gctcaccgaa
      421 tcggctttgt tgaccgattc gagcacgttg cgggtccctt ggacggcggg cgcgatgagc
      481 tgggcattgg cgtctttgat cttgccgatc agaaaaggtg aagcggtgtg aattactgca
      541 ccggcgccgc ggaccgcgcg gtcgaagctt cccggttcga ggagatcggc cttcacgagc
      601 tcgagcgttc ccggaaggcc ggcggccatg tcgagcaggt gatggcactt gtcttgattt
      661 gaagggtcgc gcaccgtcgc gcggaccgtc gcgccacggc tcagaagctc gtgcactacc
      721 caagaagcgg cgtagcccga agcaccggtg acgacgaagg gcgggtggga cgaaacttgg
      781 gtcataggtt gcctttgatg cttcttggcg atgagcgact cactgcaagc tgacggtcga
      841 gaacagtgcc cacatcgcgt cgtttgcgtt gatgtccgtg gtggccgtgc cgaggttcac
      901 gccttcgatg cgagcgcagg gctcgcccgg ccagcagtgg cctccgccct cgacggtaca
      961 gagcgtgacg gaggccccgt ccgtgcacgc gtcgcgcgtt tcgcaggtca ctataccctg
     1021 ctcgaacgtg acctcgggct catcgatgca gccgttctgc tcagcccaga agtccatcga
     1081 atcggagacg ggcatcgaac ctgccaggcc cccgccctcg aaaggcacca ggaagtcgtc
     1141 ggtaccgtgg aactggatca tcgggaccgg gcgcgatggg ctgcagtcgc tcggctcgag
     1201 cccgagcacg ccagacacgg gcgcgatggc cgcgatccgg tccgagacct cgcaggcaag
     1261 gcgatgcgac atgaagccgc cgttcgacat gcctgtcgcg tagacccggg acagatcgat
     1321 gcagccacgc gcgccgaggt cgtcgaggat ggcgcgggtg aatccgacgt cgtccacccc
     1381 gctcatggcg gactctccgc aacagaggcc ggcgttccag gagctgtcga ggccgttcgg
     1441 ataggcgacg atgaagtcat gcgcgtcggc ggtttcgtcc atcttcgtgt agcccctctg
     1501 ctgggttccg tttgacgtat acccgtggaa gttcatcacc aaggggaacg cttggcttcc
     1561 gtcgtagcta gcagggacgt gaagctcgta acttcggatc aggccctcga tcccgaggtc
     1621 gatgtgcgtg acactcgaat cgagcatgcc atcggtgcag ccgagactgg cctgggacga
     1681 ttccgtcgat tcgccgctgc tgtcgcagcc ggccaagact gtcatcaatg aaaggacgac
     1741 tcgtgaaatc cggttcatga gaccggcatc ctaacacgag gtagcgcgct gcgggcgagc
     1801 ctctcgtcga ggcgccggtg ccactggctg agcgcagctt cgaaccgcgc aaagcgaaag
     1861 aactcgttgg cgccgctcgg cattccgcgc tggatctgct cggcgatgac gaagtcctcg
     1921 tcgagtgtct tttgggtgaa ggcgtggttg gcggcgagga atgcctgagc cttttcgctg
     1981 taaccaccgc cgcccggctt gggcgcaatc gtcatgatct cgatgcgcgt ctggtcgact
     2041 gaaatcgggg tcgtgaggat caactcgaag tgcctctcct gcatcaagat cgaagcgttg
     2101 ggcgagatcg catagagcag gtgcgtgtgt tcccggatgt tccaatcgga ttccggctga
     2161 ttgcgaagct ccaagatgga aacgcggggc agcaccgagc gcacgtggtc cccgatgcac
     2221 tcgaacgtcg aagtgttgtc ggtgaagaag ctaccgatgg tgtttcggtg ggcgatccgg
     2281 aaatgatacg attcgaggcc cccatcgacg atcagcttcc aattggcgtt ccaggttcgc
     2341 gcttcggacg cgaatacggc ggcctcggat gtgccaaagc cttcgagctc tacgatcaat
     2401 ctgcgcgtcg cttcgtccgg tgcccgaacg ggcacgctcg gatccgggca gacccaaacg
     2461 agaccggcgg cctcgaaaca cgaaagtgcg acgagagaag tagactccaa atcgagcgta
     2521 gggaaaccct cctgctggcg gacagcggcg agcgaaccgt ctgtttcgta cgtccatgcg
     2581 tggtacggac agacgaagcg ccggcagttc ccccgctccc gaagctctac cgtcgcgccg
     2641 cggtgcctgc acacattcag gaaagctctt gcgcggccgt ccttgtcgcg cacgagaatc
     2701 acgggtgccc cgaccagatc gcgggtgatg aagtcgccag gagtcgcgat ttgactgcta
     2761 tgcgcgagca cgttcatcga gcgccgaaac aagagctcgc gttcctgttc gaacctcgct
     2821 tcgtcgatat actcagcgac tggaatcaag gtttcctcgt cgctcatgaa gggctttttg
     2881 ccttccacta gctcgaggca ttcggaaagg agctcgattt gcttgtctct ttccatggag
     2941 tctcggctcc agtcttaaca cgttctcgca gcggaccggc ctgctcgatt tcagccaagc
     3001 gctgccaatt cgccgggagt cgcgaccggc ggagcgttcg gcgcgcgatg gctcattcgc
     3061 agctgacggc gcactgagtg gtgaagccca ctcgtatcgg aagccggaac gcattgctgg
     3121 cggcagcgga ccaggaattg tcggaagtgc aattgacgcc ggcgcccgag agctggagcg
     3181 tgacgacgac cggcgcgagc tcgacgaagc cccccgtccc tgaggtctgg gtttcggtga
     3241 gggacgtttc gggaacgccc gagtcattca cgtaaaagct gcgcccgttg cttccgaaca
     3301 acgcatagct gaccccggcc agagaattgc catccgagac cgatccttgg aaggtagtga
     3361 cggtgagcga ccccgtgccg tctgggggat acgcggtgcc gatctccccg gcgagctcgg
     3421 agagaaccgc atcgcttgcc atgatggcat cccccacgag gtccttggtg ccggagaagc
     3481 gagcgatgag caccgagccg aaaccttcct tgacgatcga aagctcgacg tttcggtttt
     3541 ggaggagcca aagtccgtac tcaccgttct cgtcggtgaa cgcacaattg aaggacccga
     3601 actggcagat ctcgacgtca gcgatcggaa cgcccggtcc ctgcgagtct ggttcgaatt
     3661 cggtgatctg ccccgtgagc gtgacgctcg aggagcccgt gccctcggag catgcgctcg
     3721 tcgcggtgac actcagaagc gcgatgatga gcagggccga aactcgggcg gcgattggca
     3781 tgacgtccgg gacaagaatg atcctacatg cgtccgcagg caagcttcgt tgcccctgcg
     3841 cgggccccct cgaaatcccg gatcgacgat cgtgacctct gctagaaata gagctggatc
     3901 gcggggccga gtgtttggat gcgttcggtg aagaactggc cgttggggga gcggccgatg
     3961 tagtacctca ggagaaagtc gacgcggtaa ctggattctc cgatcggatc gagcgtgagc
     4021 cctgcgacca gcgaagcatc cggaatccaa tcgctctcct gccagcactg caggttcacg
     4081 gccaccaaga gttgcaggcg acgcttggtc tttgcggtca cggggcgaaa ctcggagcca
     4141 aactgcaggg accagcggtc gaggttcgaa gggtcgctgc gcacgatgac cgcggcgccc
     4201 ccgtagactc tcgcccaggc aagcggatcg taagagagca gaacgctgat ctcctcaaac
     4261 gagagattga tacgatcgac ttcgttattc agaaggaact cgtcgccgag atgcgagctc
     4321 tggtgcatgt agcgagcgaa gagcacgagc gaatcaaacg cgtacgagaa cgtaatcccg
     4381 ccgaggtagt ccgcattgat gaggtcgctc gagggcgccg caagatcgaa gaccgcgaag
     4441 attccgccct gaaagccgat ctcccaagcc cccggacgcc gggagttttt cttctgaacg
     4501 atggggatcg aggcgcccgc ctcggccgag ccggcatggg tgagatcgga gtcgccaaaa
     4561 tagtagttgt aaccgagcgt gaacttcggc cagcgcagat ccgcagcgag cggtgcaaac
     4621 agcgagacac gcgggaacac cctcacctgc gtgtcttcgg cggactccgc cgacgccgtg
     4681 ctcagagtcg gtgtgaccag agcggcgaga aaaacgagcc ggcccagctt ggcattcgtc
     4741 attggcagcg acgataaccg ccgagctttg cggggcaagg caccacgctc ggctagttct
     4801 ttccctgcaa acgcatcgag acagttctca ctcggctcgt gagcggctgt atgaacgctt
     4861 gccaatgcgc gtcagcccat cgcgagaagc tcgggctcgg caaggccgcc tcggcggtct
     4921 tctggagcac tgcgacgtgg tcgccctcct cgaccacttc gacgagactg actgcgcccg
     4981 ggtatgcgct gagccagttg cgaacgatgc gccgttcggg ggcgcgccgg tagtcgtcga
     5041 gaaagatccg cgcgccgatc ttcaaggatg gcatcgcctg atagagacaa gcttcgcggc
     5101 cgcggcgcgt ccaatgaggc ggtccgtcca cgagcagagc gtcgaccgca tcgggcaagt
     5161 cgcagctcgc atagctgaga tagggagccc cgccgtggcg ctgccatacg agtgggcggt
     5221 aaaccagctc cactctcgcg ttcggaggca gctgggcgcg cgtgcgctcg aagtacgtgg
     5281 cgtcgttgtc gaccgagagg atcgttgcat cgggaaggtc acgggcgagc gcagcggtgg
     5341 acgcgccact cccaaactcg acgatcgtcg gtgcttgcat cgcgccaagg tggcggagaa
     5401 cctgggcata ggcgcgctgg ccgagagccc acggtgcgtc gagcggagag agatcggtca
     5461 ctcacgcttg gcatggattg gagctgacgg actgtcaacc ggctggcacc agctccgcga
     5521 tgcatgtgag tagcgcttcg gtcacgatcg gggttcccgg ctggaacggc ggaacgccac
     5581 gcacgagaag tgtttcgaag cgcttgattg ccgggatgtc cagctcttcg gcagaggctg
     5641 caatcgcgaa tccgtcacgc tcggcgagcc gggcgttgag ctcctgctcg atcatctggg
     5701 ccggcgggag aagcagcatg ggtcgaccga gcgcaatcga ctcgccgatg aggttggacc
     5761 cagcggtcgc aacgacgccg cgacaggagc gaagcgcctc ggcgaatcgc tgcacgtctg
     5821 gcggttcgac gcgcacgccc tccggaaggt gctcggcacg accgaagacc tggagccggt
     5881 gcccgcgctg gtgaagcgcc cgaacgtacg cggaaaggtc agcctggccg gcgtacacca
     5941 ggagcgcgtc gccgcgcctg ccgtcgcctt cgaagagatc ggggcgagga tcggggcgcg
     6001 caatgacagt acgcggatcg aagggttgca gctcaccgaa gtgtacgacg atgatgcgat
     6061 tcgcgaccca ggacgcgctc gccgcgttca gcgccgcgtg catgcgtgcg tgcagcggga
     6121 tggcgatggg cagccggcaa tgggcaaaga gcaagccatg ccctatcgcg atgctcggga
     6181 tgcgcgacag agcggctgcg tggagtgaag gtgcgtcgcc gtccgtgatg actaggtcgg
     6241 gctgaagctc gcgcaggaga cggcgatccg cgttgaatcg agcggcaaaa cggcgcgcga
     6301 gtcgcggccc cgggtcgaac gtgtcgatct cgacgctacc gatttcgcga agcatcgcga
     6361 gcgcgtcctc tcccgaatgc aggtggacat catgaccgcg tgcacggagg gccggaacca
     6421 gtgatcgcgc acgcatcgca tgaccgcgac cgtttccgtg cacgaaatag gcaatgcgca
     6481 cgatccccgt gtacctctgc gatgaagcgc cgtccagccg gacgggctct tggcgcctgt
     6541 cgcggcgacg acatcccgcg ccccggctca gtcgatcgtt gggagcgggt gcggcttgga
     6601 cctccacacc atggcggcga tgagtgagcc cagcgcgccg acctgtagaa gcgccgtgtc
     6661 ggtcaggaac agggcggtca cctgcgcttc cggggcatcg atcgtccccg ctagaccgag
     6721 tgccagcgag agaaagagga actctcgcgc cggaacgaaa ggtgtccggg aaaccagaag
     6781 gtccacgacg agcaagctga tccaatcaga gaagctcgag ccgggcagac ctacggtcca
     6841 ctggagcccg agcaatacga tcctgagtat gcttcgcacg ccgtgatagc cgaagatccg
     6901 acacatcacg ggcgtgtcga ctgcgagaac cgttccgcga aatcgaatca ccagcaaact
     6961 caagacggcc gtcgcaagcg tgactccgat gatgagaccg agcacgcccg gtcgcaccga
     7021 ctccatcaag gagaggtcgc cgtatgcgag gtaggctccc aggaagagca cggcgatcgt
     7081 gttggcggct gctgcggaca acagtgtcac atccttggct gcggagaatg ccttccggta
     7141 cgagacgcga agcgtattca cggaccagag caagaggtaa acatcgccag agcccgcaga
     7201 aaaggagctg ttgagcaggc gcttcatcaa gaacgcaccg aagtggcgaa cgaggttcgt
     7261 cgcccagacc gccgaatagg aaagtgcgtc ggtgaccggc agcaaaaaga agcgggcgac
     7321 gaataagaca tagaaccagg gcgaacgagg caccgcgcgg aggacctcgg gccagtcggt
     7381 tccggagagc tgtcggacga gcacgaagcc gatgacggcg aagaagacca ccgagagcgc
     7441 gcgacgcagg cctttacgca cccgaggagg aacggcgtct cggatgcctt cgccgaaccg
     7501 ccgaatctcg ctgagtcggt caccgaatcg gtcgcccgga tctacacccg acgggtcgcc
     7561 actgctctgc tcgcccaccc ccgcgttatg acccagaggt tgcgctggtg aaagaaacgc
     7621 ggcgaagcgt ttgatatcag ccttccgctc ctaagaaagc tgtgcgaggc tgtcccgtcc
     7681 tcgtcgaaca cccggagtga accgatgcca tcgatcgtca aaacctgact ccttgctttt
     7741 gcgctgccat gtctgcttct gaccatgttg cccgcctgcg ggaacggcgg aagtgacggc
     7801 ggaggaggcg ccggcggtga accgatgacg tcgacgttcg agttgcaaac cgtggacttg
     7861 ccgagcgatg ccaggcccgc ctacacgccg ggcagccccg gggtcacggt gaccaatccg
     7921 aagctcatca cgcagttcgg aagggcggat ttcagtctca acaacgcgcg gtacacgcgc
     7981 tactacttgt ccgaccaggc cgacactcag ccggacgcga tcgttgtgct ggtgccaggt
     8041 ttcgagggcg gcgcggcgac gttcgcggtg cttgccgaga gtcttgcccg gcgtgcccag
     8101 cgcgagtcgt ccatggtcgt cgaggtctgg gcggtcgatc ggcgttcgaa ccagctcgag
     8161 gacaccgcgg gcttggacct cgccgagcag gagcaagacc cctcgctcgg tctcgacttc
     8221 ttgtttggcg aggagctcgg gctcgagatg aacgagacct tggcctcgga gctcaaccgc
     8281 cgggccgtct tctacaatac tggggaagac cttgcgttca tggcccagtg gacgacgctc
     8341 gtgcactcgc aggacatcga cgcggtggtg gaagaagccc gcagcaccgc acgcaacgcc
     8401 aatgtgttcc taggcggtca ttccgcgggc acgggcttca cggcgcgcta cgcggcgacc
     8461 gatttcaacc tggagggcgg cgatccggaa cccggctacg ccaagctgcg cggcctggtc
     8521 atgctcgagg ggggcggcgg tagcctgcca agcgaaagtc cgagcgaaga tgagctcgac
     8581 cggatcgaag cgcgtttcga cggagggctc tacgaagcgg tgcaaagcca agagccccgc
     8641 tgcatggacg gcgtgaccgc ctgcacggtc gaaacggagg cagaggactg cgacgggttg
     8701 ccgaacacct cgtgcaagcc agtcgatgcg tattcggtgg tcgcagggct cttgtcgcct
     8761 cagctcttgg cggtttccga ggtagctgcg ctcgagggcg acctccaggg cgataccgga
     8821 atttcgctca tgcaggccga tcagaatggc atcgaaggca acaacgcggt gagcaaggtg
     8881 cccgagctcg ctgcgctcac cctcttgcta gggaccgcac ccgccacctc ggtcgccttg
     8941 atcggcttgt tcctggacga tgacggggca gccgctgcaa ccgccagctt cgtggcaacg
     9001 tcgctcggca cgatcggcga aaccgtagac ggtgtgcgca cctggttgaa ctacggtgac
     9061 ccgatgccgg cgagcgtcct gaccgacaac ggcccggcgc cgactgagct cgcggacgcc
     9121 ggtcgctggg gcgtcgaggt cgagtccacc gatctcgaag ggcacatgat ccccagcttc
     9181 taccaggggc agaccaactt ctcggattgg tactatccga gctccgggct cggggtcgta
     9241 tccggtctgg gtctcgacac cacgccgctc tcggcgccgc cgcccgaggg gcgcggccgg
     9301 agcgatatcg agaacatcac gcaggcgcgg aacgtgaaca tccccgtcat cgcctttggc
     9361 ggaagcaacg gcctggttcc agtgccgggg cgtatgctgg cctatgcgga ctcgctcgcc
     9421 gcctgcgccg cgccgagctg cgatggcacc acggcgcggg tcgtcgatcg cgaacagcca
     9481 agctcggcat tcccaacctt cggtgatgct gccggcggat tcgaggtctt catgtccgag
     9541 gggtacagcc atgtggacgt cgtgtccgcc gaagacgacg agaccaacaa cgtcgtcgcg
     9601 ccgctgctgg cgttcatcga gcgcaacctg gagtagctgc ggcatcagcc ccaaagctcg
     9661 tagagcgaga tcacgatctc gaggaggatg agcacgacga tgtaccactc gactcggaga
     9721 ctctggctgt gcagccggag atcgaggagc acgcgggcgg cgctcgacat ga
//
LOCUS       DASRZF010000105        51428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4546019, whole genome shotgun sequence.
ACCESSION   DASRZF010000105 DASRZF010000000
VERSION     DASRZF010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 51428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51428
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4546019"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="VFG22_04655"
     CDS             complement(<1..606)
                     /locus_tag="VFG22_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415562.1"
                     /translation="MTSVWTLAYGASTSSADASPRSPAAIDETGVLDAVRAGQRMGPK
                     LLQPPPSDGLGLDIAIEDRDGKSMLALHRALQRAESGEGKARLLFFGASHVASDLFTG
                     HIRRELQSRYGDAGHGFLVPVHPWRTYRHRDINIESDGDRWETQRIRVGDTEVERLGL
                     AGITMMSQRLGSYGAVFTAQESDYGRNASFFELYYMKHPRAG"
     gene            705..1259
                     /locus_tag="VFG22_04660"
     CDS             705..1259
                     /locus_tag="VFG22_04660"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET6415563.1"
                     /translation="MKARPKSAMMRAMKSRELLLKAAKFDVARLRFEGPDGKEIVRDL
                     VEHPGAAVILPQLDDGRIVLIRNLRRTVGKILWELPAGTLDPGESPEVCAARELEEET
                     GYRAGTLAPLTAFFASPGILNERMHGFLATDLVPTAQNLDQGEEIQVFPIPQWQVRDM
                     LKDGHIEDAKTIALLLYWMHLYSP"
     gene            1280..2518
                     /locus_tag="VFG22_04665"
     CDS             1280..2518
                     /locus_tag="VFG22_04665"
                     /inference="COORDINATES: protein motif:HMM:NF016196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HET6415564.1"
                     /translation="MDLLSRIQDNWIVLLIPLISALVGWFTNVVAIKMMFYPVEFLGI
                     PPYLGWQGVIPANAVRLAKVSNKLITQKLLSLRELFDESFNAESFAGKLGAVIDDVTE
                     QVIDEVANKHAKAMWDNAGDFMQNKVREKVRSEVVEVSRAIAMDMADDIDEIMDIEQT
                     VLEAMERHKGLMGEMFYEVGRLEFKFIERSGLYFGFLFGVFQMIVWVLYPAPWILPAA
                     GFFVGYLTNWIAIKLVFSPREPTKIGPITIQGLFHKRQNEVAEAFGRTVATRVLNADN
                     IVTTVMESEGAKRMNEIVAHRIGELISKYEAHPMAKLVVPEASQPALRAEVLERIQTE
                     WPKPGGFFHTFAGEAVDLQGELERRMKALDRESYEGVLRPAFQQDEWKLIAAGAVLGT
                     IAGVLQLVYIFGDAMERLSR"
     gene            2567..3421
                     /gene="purU"
                     /locus_tag="VFG22_04670"
     CDS             2567..3421
                     /gene="purU"
                     /locus_tag="VFG22_04670"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HET6415565.1"
                     /translation="MEQTATLLVCCPDRKGLVAALAQVLYGHGANILDADQHTDPVAG
                     QFFQRIKFDLSELHTDRTSLETAISEVAQRFSMNWRIADGRSRSRTAVFVSKYDHCLY
                     DLLLRQRAGELSTDIPLIISNHPDLEPVAKQFNVPFRHLPIAKGAKRAQEEEALALLE
                     AERIELVVLARYMQILTDDFLKAFSGQVINIHHSFLPAFMGSKPYHRAFQRGVKLIGA
                     TAHYATADLDEGPIIEQDVVRCTHRDSVQDLVRKGRDLEKVVLARAVRWHLDDRILVY
                     DNKTVVFS"
     gene            3431..4225
                     /locus_tag="VFG22_04675"
     CDS             3431..4225
                     /locus_tag="VFG22_04675"
                     /inference="COORDINATES: protein motif:HMM:NF005699.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HET6415566.1"
                     /translation="MSERVQLSIEEHVATVRLSRPEKRNALDAEMFEGLIGVGKRIME
                     DKRVRAVVLHGEGKAFCAGLDFGSFLGSPQLMQKLLSRSEESPANMAQRAGWIWQEVQ
                     VPVIAAIHGFAFGGGLQIALGADLRYVTADAELSVMEIKWGLIPDMSITQTLLHLVPL
                     DVAKELTFTGRIISGTEALGLGLATKISVDPLADALETASLIASKSPHAIRAGKALLR
                     DAPGMSRADALKLETALQVTLLGSPNQTEAVQANMMKRKPDFKDPE"
     gene            complement(4233..5276)
                     /locus_tag="VFG22_04680"
     CDS             complement(4233..5276)
                     /locus_tag="VFG22_04680"
                     /inference="COORDINATES: protein motif:HMM:NF000689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HET6415567.1"
                     /translation="MGDSDLEYDSVLLVSFGGPEKRADVMPFLENVVRGKNVPPDRLK
                     EVAAHYNHFEGKSPLNDQNRALIAALEKELEESQIDLPVYWGNRNWDPFIIDALRQMR
                     DDGKKRALAFFTSAYSSYSGCRQYRENIQKAREEIGEGAPIVDKLRAFYNHPGFIEAN
                     VDGLLEALETLPKERRECARVAFSAHSIPISMAENCDYTEQLQETARLVADGARHERW
                     QLVYQSRSGPPHIPWLGPDILEHLDVLAQINVKDVVVAPIGFLSDHMEVVWDLDHEAR
                     DYANDLGITLTRAPTVGTHPAFVRMIRELIEERLGKVSTRQAAGNRGPSHDVCPADCC
                     QYHPRVSRPPDAT"
     gene            5357..6139
                     /locus_tag="VFG22_04685"
     CDS             5357..6139
                     /locus_tag="VFG22_04685"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6415568.1"
                     /translation="MRVTILGSGSEGNALLLESGATCILVDAGLSYRKLVQRFAAIGR
                     GVPSNVAAVIITHSHGDHAAHASTYASQFACPIRASEATLKSIRLRPSVASETFDVGR
                     CISIGEISIQSRAIPHDAPQVALRFENKQTSVGLVTDLGHVPKGLDRFLDGCETLLLE
                     SNHDRDLLAEGPYPDVLKRRVGGSLGHLSNGQAADLLAELRRAPPRVVLMHLSETNNS
                     TRLARSSAEAALGHQGTKLLVAHQQRPLELDPVREAQMQLGL"
     gene            6150..7004
                     /gene="speE"
                     /locus_tag="VFG22_04690"
     CDS             6150..7004
                     /gene="speE"
                     /locus_tag="VFG22_04690"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002010.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HET6415569.1"
                     /translation="MRAWYDEIFEDHTRLGLRVKETLYSGASKHQEIEVLDTIGFGRV
                     LVLDGVFMTSELDEFLYHELLVHPAMTTAPEIKRVLVIGGGDGGTVREVLRHSGVERC
                     VMIEIDPMVVEVSKRYLSEIGTAWHDPRLDVRFIDGVEYVKLSDDEPYDVILLDGTDP
                     VGPGAALFDESFYAGCKRMLVPRGVMALQSESPFMMMDVFVATQNKLRKLFSHVHPYL
                     GPVPLYGTGTWSWTWCSDMGRPLEAITHRQEAVVQGSKAYNADLHQAAFALPNYVKRA
                     LDPEDVSA"
     gene            complement(7041..8027)
                     /locus_tag="VFG22_04695"
     CDS             complement(7041..8027)
                     /locus_tag="VFG22_04695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HET6415570.1"
                     /translation="MVTARAIRIREPGGPEVLELGEIELPSPGPSQVLVEVAASGLNR
                     ADCLQRRGFYPPPPGVPVDVPGLEFAGVIESVGESVTAWRPGDRVMGIVGGGAMATHL
                     LTESAELMPVPKSFSLEQAAAVPEVFLTAYDSMVLQGGLGAGKSVLIHAVASGVGTAG
                     IQIASALGAISVGTSRSAHKLDRCSKLGLTHAVLVEDGNFADEVLAAIPDGADVILDT
                     VGAAYLAQNLKVIAKKGRIIVIGLMGGVKAELALGALLAKRASIQGSVLRSRSAEEKA
                     ELTKSFTGEMLGRFETGELKPIIDDILPMTEIQAAHRRMDANDTFGKLVLRW"
     gene            8092..9621
                     /locus_tag="VFG22_04700"
     CDS             8092..9621
                     /locus_tag="VFG22_04700"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET6415571.1"
                     /translation="MNANVGRFLHHWALQDPERTGILDSGPGEIRYSYGELDREARRV
                     AGHLRSRGLGLHDRVAICTGNGLHFVAAWFGAAYAGCTTLPIPFMSTAHEIAFRLQHA
                     RCKALLADAERSAVAKESQLRAGGFCALLSIEEALTGNVSEEAPCHAPADAFAMLLYT
                     SGTTGAAKGVCITHGSLGAHTRALAEQTLELTSEDRILGTLPLTHSYGIRTTLLAPFF
                     AGASTVFVPRFSAALTLELCTRHRVTWLPAVPTMLVAWANEPSGPVPPALRWCLSAGA
                     PLLENVRRRAEDRLGAPVRQGYGLTEANLAAINAPPDRLLEGSSGKPVPGVEIRIAGD
                     SEEELPPGERGEVLIRGQNVMAGYLDDKIASAHVMRGGWLHTGDVGFVDGEGRLTIVD
                     RSKDLILRGGASIYPSELENVLAHHSAIDDVAVVGVPHDYYGEEVIAVVVRSMKVTTR
                     ELDAWVRERLAPYKVPRGYAFIESIPEGPSGKRMKRRLRDQLVAGEIEIERLATHGTA
                     H"
     gene            complement(9595..11310)
                     /locus_tag="VFG22_04705"
     CDS             complement(9595..11310)
                     /locus_tag="VFG22_04705"
                     /inference="COORDINATES: protein motif:HMM:NF015515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HET6415572.1"
                     /translation="MRAVLLVLMVVVGFGAAKVGATSSDGDDPRLIQIASSNTIPLAS
                     VEVAAGGGESALPAWTSILPPLLAIALALLFRHVLVALVLGVWLGAFLLSDLAPLASF
                     ARLLDTYIVHAIADPGHASILVFSLLLGGMLGLMTQSGGAKGLASWVGTDSTSRRRAQ
                     LTTWFLGLLIFFDDYANSLLIGSSMRPVTDRLRISREKLAFLVDATAAPVASLALISS
                     WIGVEVGYIGEQLTQLGIERDAYVTFIETIPFRFYPILMLLFVAWIGLRGRDFGPMLR
                     AERRALESGSVLRPGAQPASDFHEELVPDEVPGRPMNALIPVLVLVLVAALGIFVDGR
                     AKVLSAGLEPSLRAIVGEASSSAAILWATGAGCLSALALSVGTRALSFGESIEAWMSG
                     MRSMLFAAVILVLAWSLSAVCRDLDTASFLIAVFGDWIAPTWIPAAVFVLAALVSFAT
                     GTSWGTMAILFPLVVPLAHRIAPGNETILLGTVSSILAGSVWGDHCSPISDTTVLSSM
                     ATSCDHMDHVRTQLPYALFIGAVSLLCAELPVAAGWYGPWVGLLLGAAVTIGVFELVS
                     RPVSR"
     gene            11354..11998
                     /locus_tag="VFG22_04710"
     CDS             11354..11998
                     /locus_tag="VFG22_04710"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET6415573.1"
                     /translation="MTPALALLFVALAPLAAPTRALSSNDELTTEDKKQLAAGELVTK
                     QKHEQRGAYKLIGGQSWQIINVPVDVAWQAIADLSGYKNFIPLATESDVKHQAGEETD
                     LAIRQQWGPIDVHYVLQATLEPERRAMVFRVDHSKEHDIRAGWGFMRVRPFKGDRTLV
                     SFGALVDIGDGVFVSVIRPAVQKDLLRIPYFFKRHLEGIDPKSEAELPRRNQSN"
     gene            complement(11995..13407)
                     /locus_tag="VFG22_04715"
     CDS             complement(11995..13407)
                     /locus_tag="VFG22_04715"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET6415574.1"
                     /translation="MPQTSAVSTSSSQRQRQASGASGALRAHFDSGRSKPLSWRLSQL
                     DALEHLLIEREQDILDALRADLRKPALEAFTSEIGMILSELRLTRKKLASWMKPQRVR
                     TSLVAVPGRSFVLREPLGVVLIIGAWNYPFHLLCLPLVGALAAGNCVVLKPSELCPNV
                     SAFLAQWIPRYLDPRAVRVIEGGVRETTALLRERWDHIFFTGSAKVGRVVMEAAAKNL
                     TPVTLELGGKSPCVVDETANLDLAARRIVYGKFVNAGQTCIAPDYVLVHEQVHDALVN
                     RMVSAIREFYGDEPHESPDLARIIDREHHARLTGFLQGADVVVGGESDPADLYIAPTI
                     VKNAKEHDALMQEEIFGPILPVISFQAIDEALAFVNRGEKPLALYLFTSDSQVEDRVL
                     SATSSGGTTINHVWLHFGVPELPFGGVGESGMGAYHGRHSFETFSHRRAVLKKRALWE
                     PALLFPPYSARKLSWIKKLI"
     gene            complement(13635..14555)
                     /locus_tag="VFG22_04720"
     CDS             complement(13635..14555)
                     /locus_tag="VFG22_04720"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HET6415575.1"
                     /translation="METRALEAATPHVSLSRVEQWQRATIFVLSHLAVLGAFWSGVTW
                     QALVLCFVLLFVRLWGVTAGYHRYFSHRTYKTSRSFQFLLAVLAQSSSQKGVLWWASH
                     HRVHHKLSDQPGDVHSPVQNGFVYAHLGWIFDGTEETRWDKIRDFAKYPELRWLNKYW
                     AVPPAALALVSFLIAGWPGLFIGFFGSTVLLWHNTFLINSLTHVWGTRRFETTDQSRN
                     NIVTALLTLGEGWHNNHHHYQSSCRNGFYWWEIDVTYYVLKMLSWVGLVWDIRGVPEH
                     VLEEGRRRDRGQVEASQDCAEPAKKLSLAA"
     gene            complement(14647..15360)
                     /locus_tag="VFG22_04725"
     CDS             complement(14647..15360)
                     /locus_tag="VFG22_04725"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6415576.1"
                     /translation="MTRNEAQRLLIVEDEEHLAAGLKLNLELEGYRVDVAGNAKEAGQ
                     RLLDPSGYDAIVLDVMLPDVNGFELCKKFREAGNFVPVIMLTARSSPEDRVLGLEAGA
                     DDYMVKPFELGELLARVRSVLRRQRWEQVSGTQSKATMLSFGGAEINFDTHEVTVSGD
                     VVQLTQLELDLLRYFAENAGRVLSRTELLERVWKLRNYGNTRTVDNFISRLRRRFEQD
                     PSSPVHFLSVRGAGYKFVA"
     gene            complement(15357..16301)
                     /locus_tag="VFG22_04730"
     CDS             complement(15357..16301)
                     /locus_tag="VFG22_04730"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET6415577.1"
                     /translation="MARRRRFRSSLSIPITLGAITVLLSMALLVGWSFLLGQKITRSA
                     DVAGDVWLLVLGALSFVLIIVVLVLFVISLARGIIEVRKQDTFIDSVTHELKSPLASL
                     QLCLETLGREGLEDEAREKLRHMMLEDVDRLRAFIDDVLEANRLSYARQGMMNLSDVS
                     LHQLTEQCAEVVRARHRLDPEEVQIDVDPALIVTTDRAALEIVVKNLIDNAVKYSARP
                     AHVRVVARRDSEKKMALEVRDGGIGIDRKNLKRVFHRFYRVEEQEVRERRGTGLGLFV
                     VWALVKQLGGDVRAFSEGRGQGTTMRVELPLAHAVNAS"
     gene            complement(16320..17087)
                     /locus_tag="VFG22_04735"
     CDS             complement(16320..17087)
                     /locus_tag="VFG22_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415578.1"
                     /translation="MLQLSFEFAGASPTIAERGVPERREDELDRAYARASDWSRALAA
                     RVGQPIRLVVTDNRSTMLTARSKGGRLEVRIHHMFLTAEEDILAAVGDYLSDSDSQAA
                     SAIDRFVEDNRLRFVAPGPPQDTLRSAGRHHDLRAIADDLARRHLGSALDVRITWGKR
                     VQPKRRQRSLQLGTYLPEERLIRIHPVLDQSWVPGFFVEAVVFHEMLHHDMPAVVQNG
                     RRHYHTRAFRKRERSFEYHSAAQQWQTDNLWRLLRGK"
     gene            17168..20515
                     /locus_tag="VFG22_04740"
     CDS             17168..20515
                     /locus_tag="VFG22_04740"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6415579.1"
                     /translation="MRAANRDRLEGLTVLHRLPSSEQRAAAWRQGIATLARAVVDERR
                     PVPLEGLDPNALLQSLEVADTEGLVEQLDWISGPAAAAALYEVAAALPSSEIKRKLGR
                     MVLQRLRKGDAATFVSVATQLALGSQRALTGKSIRARVALALSLPIGTVNNVDALALA
                     LISRQDTSRDWLRTPATGSLPSRRLAARLLERAAREAARRAAEGDDTGVRVFCTEAVR
                     EPWGRLLGDRENLVWRHVASARGLLASSMPIFREEMQRHLNPEFTITEWRRAATSLAA
                     SIAVEPDEGLRACRALLESVVFQQDHGIAAAMISGLPRAAESHPVAVEALLEQLVRVG
                     GLDVAEALVELRREQVHPGFAEEAARKAETHLRKAIAQGGNEDLGRTALMKSLADELH
                     GARRSSEPALRDMLSQALQTFANQGAYEAAREAPSILEAAGRKAKLLEKPTAGDPSSS
                     IDSFLALRELDSALFETSALNDLLLVGEDDAGSAIHDERLGDLFQKVTNWLIIREGDP
                     LQEGAQVPQFTTRLRQLRTMLHLVDADGPSVDSRRDLVRQRRLLTQRVLLNRVRHDPK
                     SSLRRTLCAATARACDALVREDIADVSDVVIAAAGNVPDLDDLKTMAEASMVPEVESA
                     LRAYAKLQQASRTAQRDRQGLMAATDALKAIGNDLPVARSAHIEAFRRSLSLLASGLR
                     SAAASSSLAEVAERSSGTPLSKLERGVATMAQLVSGSRRRLGASVGDAFPSSAEAVWA
                     MGLELERALRASMSELDQASSDAVSATARDLPGAFGEITGAVLRHLGALPLDGPRRAP
                     SIAPLELDADASLPAWAPPSRIIGGFHLVRTVGTGAVGSVFVARRVEEGQDDSAPVFA
                     LKVPDYSAAAARTLSEDEFLRLFREEAGALLALPEHPNIARFVTFDAGARPKPILVME
                     LVEGPSLERMLEMGDLSLGRARELLLGVGAGLDAMHQVGVAHLDVKPSNIIVRNANAI
                     TGVGQPPQPVLVDFGLAGRHLRPGCGTANYGAPEIWGAGDVSQSIPADVYAFGCLIFE
                     AYTGKTLFDAGGEAAMITQHVTHDGDPEPVLSLLERPETRELGALVARCIRRNPSDRI
                     TMRQARQFLANLSLGAADQHWPVRIAS"
     gene            complement(20609..22411)
                     /locus_tag="VFG22_04745"
     CDS             complement(20609..22411)
                     /locus_tag="VFG22_04745"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET6415580.1"
                     /translation="MKKSDDLRESNRGWVAPLLAMFVAASGAASGVSAQQGSTQIDLN
                     QYRPAELATDGFASSTADGQGHKRFGIMIYMDYNDDALVFQDQGTGNTSSVVHRQLTG
                     HINWNLGLWDHLVIYMDIPYHFIIDSGQGAGDALPGGASGPFGYLLPNGGHFGDVYFG
                     ARGNIIGNRHDVFQLALQATMTINTSSLSDDQQKYAGQVDKKPYLGGWFELLMTFNAG
                     DVVRIPIQVGYKLGTQGQTVAPDLFVGNEFTFGGGLLFMIAQDKFMISAEAFGRTAAN
                     STVPFWTREETPVEVLGGFKYLHPKGFVVGVSGSAGVAPGYGAPDWRGVGMIGYTMPG
                     EEPILDADGDGILDDVDQCPNEPEDFDGFQDEDGCPDPDNDGDGVLDADDGCPNDAED
                     IDGFQDEDGCPDPDNDGDGILDVDDQCPNEPGTLENNGCPDPDRDGDGVPDRVDNCPD
                     EPGTVENHGCQLAQLVVIGADQLEILEMVYFKTGSAKLQPRSFALLDNVAQVMNAHPE
                     IPLIRVEGHSDSTGNLKYNMRLSTNRANTVVRYLVGRGKVDKARLISEGFGPTRPLVP
                     DAKTKEELAQNRRVEFHIVDTGPEDAATETEVSE"
     gene            22560..23918
                     /locus_tag="VFG22_04750"
     CDS             22560..23918
                     /locus_tag="VFG22_04750"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POT family MFS transporter"
                     /protein_id="HET6415581.1"
                     /translation="MPPRARYRTAPAPTRGMPSGIPYIVGNEAAERYSYYGMRAILVI
                     FMTKFLMNAQGELDLMSDDQARTWFHAFVTAVYFFPIFGAIIADAFLGKYRTILLLSV
                     VYCLGHLTLAVDDTRIGLAVGLGLIAVGSGGIKPCVSAHVGDQFGPTNARLLPKVFGW
                     FYFAINVGALASILFAEPLLHRFGPSVAFGVPGVLMALATLVFWMGRNEFVHIPPGGV
                     AFLREVFSRGGLMALGKLFIVYTFIAMFWALFDQQGSSWVLQAQRMDRHFIVDWDPSQ
                     AQWLNPALVLLFIPLFNRVIYPAFDRFWPLTPLRKISLGLFLTVPAFLLPAWLEARLD
                     AGESVNIVWQLGGYIIMTGAEILVSITALEFSYTQAPRHMKSLVMGAFLMSVSIGNFF
                     TAAVNYFIQNPDGSSKLDGDSYYLFFAAAMGVTAVLFVPVAVWFKERTYIQEEAPNEA
                     VL"
     gene            23915..25639
                     /locus_tag="VFG22_04755"
     CDS             23915..25639
                     /locus_tag="VFG22_04755"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HET6415582.1"
                     /translation="MSIIEQARSWIASDPDPETRRELEALLALNDVAALEERFAGPLQ
                     FGTAGLRGLLGAGPSRMNRVTVARATSGYASWLMKQVPDASSRGIVVGRDARRNSDAF
                     EREVIEVLAGSGVPVWVFEDVIPTPVLAFAVLKLRAAGGVMITASHNPPGYNGYKVYW
                     ENAAQIIPPHDQGIAGEIELAPSALEIPRISLEDAERRGLLRKADAMRGEYIEEMASE
                     VRRGQRGPELRIAYTALHGVAESTFRALMARAGFDDVQSVSEQSVPDGTFPTVAFPNP
                     EEPGAMDRVLDLAQRIDADLAIANDPDGDRVGVAVRCETGFEILSGNEIGILLADDLL
                     SVRKRSGQPLVISTIVSSPMMGPVAASHGARWEQTLSGHKWIQNRALELMEQGLDYVF
                     GYEEALGYAPSLAVRDKDGISSALRIADMASRLKARGETLLDARDELWGKHGFFADRQ
                     LSVKFEGAAAQGRMNAVVNRFRDSIPDRIGGLAVLRMLDLERQLQWTPLGMRPFADLP
                     RSNVLVFELQGGHRLMLRPSGTEPKLKYYFYAVAPHRADGGLSASKASALAVLDRMVE
                     YVRDASDC"
     gene            complement(25617..26069)
                     /locus_tag="VFG22_04760"
     CDS             complement(25617..26069)
                     /locus_tag="VFG22_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415583.1"
                     /translation="MLRARIAAWLQPAPQASEPVEGRPAYHSDIRELRPQSWSRRSPA
                     AQGSDIERALWLAGVSSRDFAEVVDIEISPHLVTPVDDIVDICLRQSQACAQQAEELS
                     AELPAPNSYGRPPLPWHRGVTRDAEKLYRASQAWDALARALLSNRWHP"
     gene            complement(26116..26700)
                     /locus_tag="VFG22_04765"
     CDS             complement(26116..26700)
                     /locus_tag="VFG22_04765"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET6415584.1"
                     /translation="MIRLRQIKGADAGREFEFEQDLIRIGRMPDSDVNFDPEVDLDAS
                     GRHAEIRNEDGRYLLIDTGSRNGTWLNGQRIKHAALNDGDEIELGRGGPMLEIASVGV
                     RPSSKRPSVNAPSDAASREPRFDPLAMPITTATRLEDLVARQDLLPESLRFSEPPESI
                     SRDSRRRRSSVVLWGAAGVVLLVALAIILRASLQ"
     gene            complement(26710..28065)
                     /locus_tag="VFG22_04770"
     CDS             complement(26710..28065)
                     /locus_tag="VFG22_04770"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HET6415585.1"
                     /translation="MPEGLHFTIFGKTDVGLIREHNEDNFMVGDLSAETGPFAGEQEV
                     SGTVSDRGMAMAVCDGMGGAAAGEVASHMAVDTLFEKLRGNEPPKDRDSFARRLVDSI
                     EDAGARIFLAAKKDRTRRGMGTTATVAGLVDKVLFMGQVGDSRCYILRNGRLSLITKD
                     QSLVNQLIEAGQLTEEEAEAFEHSNIILQALGTTEQVAVDLTFVELRRGDRLLLCSDG
                     LSGLVHDTTIEEEMACIGPLPQLADRLIELANAAGGHDNVTCIVADFDGDALEPPEAG
                     AIPFYQQYPLPRGKDGRPAGGPSLPHVVRSMVPRAMSPQRPDPATVPSEPGTHGSFSW
                     STAASVLALLGLAAVITVVSAGRRGVSTGALTQPPLPAYTDSQIPVEVEVRAALESGE
                     LFVNGRSYGPVRLEEAILLRLAPGAYYFEAREVDGTHVGKDVLVEPGSPREVVLIPPT
                     D"
     gene            28477..28854
                     /locus_tag="VFG22_04775"
     CDS             28477..28854
                     /locus_tag="VFG22_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415586.1"
                     /translation="MEIFLNKRAMRALRLSVADAIEDGEIDALRDDLVSAFSDDDIEE
                     IERRIDSGDFYDFVSEILDEWSGEDFDELIELIETQLSDADVDLKYTATEVEEDDTEE
                     ASEEEDEDDTDDELIVDDEDEEL"
     gene            28964..29039
                     /locus_tag="VFG22_04780"
     tRNA            28964..29039
                     /locus_tag="VFG22_04780"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28997..28999,aa:Thr,seq:cgt)
     gene            29667..29954
                     /locus_tag="VFG22_04785"
     CDS             29667..29954
                     /locus_tag="VFG22_04785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="encapsulin-associated ferritin-like protein"
                     /protein_id="HET6415587.1"
                     /translation="MSNEGYHEPIAELSDETRDMHRAITSLMEELEAVDWYNQRVDAC
                     KDAELKAILAHNRDEEKEHAAMVLEWIRRRDTKFEHELKDYLFTDKTIAHE"
     gene            complement(30100..30765)
                     /locus_tag="VFG22_04790"
     CDS             complement(30100..30765)
                     /locus_tag="VFG22_04790"
                     /inference="COORDINATES: protein motif:HMM:NF018684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HET6415588.1"
                     /translation="MKSFPIVTVAGTLVWMAVSTWLAPSAAATAAGPSFDCSKARGSI
                     EQLICEDEALSAVDRTMASVYAAASRKAVDERPHVLRAEQRGWIKGRNDCWKSSAPRA
                     CVEGSYQLRIAALQARYHLVHATGPMSYTCEDDSRSELVATLFATDPPTVVVERGDRV
                     SLMFLQPSASGAKYAGGNESFWEHQGEAFLTWGHGTPEIRCVRKRTQDGSTHERTTPR
                     TDR"
     gene            complement(30807..30980)
                     /locus_tag="VFG22_04795"
     CDS             complement(30807..30980)
                     /locus_tag="VFG22_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415589.1"
                     /translation="MLSVAEEIPHLYVVAEGLTETDKILVEGLRKVHDGDVISLRFPS
                     LAEMMSDPDVPAG"
     gene            complement(31296..32912)
                     /locus_tag="VFG22_04800"
     CDS             complement(31296..32912)
                     /locus_tag="VFG22_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415590.1"
                     /translation="MRVQVGRLWIFALVTLSLGGCGSDEGAAGAGGVDGAGARGGAGG
                     AGGAAGTAGTDGTGGMGGRIALPCSPDSLCETDGLLCNLAEGSCVECLSKANCAGEMQ
                     ACIDGSCRERLCLEDSECPPERPQCTDTGCAPCVGEVAPDGQRGECFDARGPNGESPT
                     RDSDGCSAQQFIAALEDAGYDIPDDPEVRQGIADNPLERVSEGLCTVPFGNENANANT
                     AGPDLRSACELHDYCYAVCGSTRAQCDDEFRSRLLDTCAATYAVGPCRITCDVLALIY
                     AKAVADQPETGWVGGQGRNCQCCPDFTTDGTCDEMAGESVANSRDCKGDLPNGASCVL
                     NTECESENCGVHGECTPPSCLANGDCSSGICNWGVCLARPLEEGSGCSTSNACTSGVC
                     TLGVCRECGRDDQCAANKHCNLLGDCIDDLGNNAVCTANAECLSNICSAGFCAECVRD
                     DQCAANKHCNLFGDCINDLSNNAVCTANAECLSNICSAGFCAECVRDSQCAANKHCNL
                     FGDCINDLGPGNPCTANAECISGTCIGVCL"
     gene            complement(33230..33949)
                     /locus_tag="VFG22_04805"
     CDS             complement(33230..33949)
                     /locus_tag="VFG22_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415591.1"
                     /translation="MRSSWLGLAALVAALLSGSFQRVPAQDVEQTSESKKGASLSTEL
                     RFRLFTASYRWSSYRSGAMDLPDSGKRSLYVVPALGVRFYPKNGHGVLVDVDYRLDID
                     VDSDAEFLCIFDCPPQLWTEFVVAHAGYGYRYVIRTARERGRLLWTLTLHASVAAGKS
                     YTDRTGSFNQSEQSAVVGARFGLDIDLHINRFFMGWTLRYEILKHTKGNLRLSHFLSW
                     NVIPVFAIGAVIGREVRAPVP"
     gene            complement(34432..36138)
                     /locus_tag="VFG22_04810"
     CDS             complement(34432..36138)
                     /locus_tag="VFG22_04810"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HET6415592.1"
                     /translation="MVGHFSHFDLQRIGRWSLGYSRFLLRYANIGCAILAPSSSFPLN
                     MMPLNRANRVEIDPASDPLERGLKSKPNELALASVKTQMSWRQLAEISDRLAVQYLHL
                     GLQPGDRVASLMPNRPALIIHYLACLKAGLVATPLNYRYMAPEIDHALEVSGASLLLH
                     HAERDEDVAASKLGNKLSKGVVRYKADDGAGRHYEELVEAPQPTATFPTPDPDAATLI
                     YFTSGSTGKPKGVTHTRATFFQMIDASINGVQFTDTDTLLPGSSLSHIAGSLLGLSML
                     TAGGRLLISRGSDGPEILPLLREFRPTVLWMLPAALIALVRDHDAGSSDFASIRLCSA
                     GGDKVSAELEREFTAAAGFPIDESYGMTEIGVSTINPPYGENRMGSIGKLIPGYEMSL
                     RDDSGAEVPVGTPGRLWIRSQANMIGYWENPKATAETIVDGWLDTGDMMRVDEDGYLW
                     FCGRKKQIIVHDGSNICPQEVEEALTEHPSIAEAGVIGIHDLVHGENVRAYVTLKEGA
                     PPPSASELISFARDRVGYKAPEEIAFLSEMPLNATGKLDRVKLLRMAEERLSASSTAG
                     PA"
     gene            complement(36310..37494)
                     /locus_tag="VFG22_04815"
     CDS             complement(36310..37494)
                     /locus_tag="VFG22_04815"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HET6415593.1"
                     /translation="MALAVVALGACCVPRAVHAQTGGDATSVQSALKQAQREAAEGLT
                     TESRLEFGDTDDWIELKTGEWLRGDLRWLRPKGLEAGQNLNFYSNKLDSLTLSWGSIA
                     GVHSPKIKSYTFKDKVKVSGKAMITKDAVIIENDQGIKTYPRAELLSISEGPPRERTW
                     WSTMLSLGFSANAGNTSQGSLTTQWSLMRMDGHTLGGLRYNGTVAYTDGELNVNRHLG
                     DVNTALFLWDRVYLIPIAGQLLYDAFQNIKLRATPSTGAGVYLFKKTRQRKKHTNQFE
                     WDLQSGLGYQFLGFLSTPTGATNPQSDGFILLRTHWKLEFLNDNVELTIDWRTNLAYT
                     NFGSTNHTGTVDVSVEITDRFSFLTTFLFLRTRDPQPRADGTVPKENDYQIVVSIALK
                     IN"
     gene            37799..39073
                     /locus_tag="VFG22_04820"
     CDS             37799..39073
                     /locus_tag="VFG22_04820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET6415594.1"
                     /translation="MPDTDRDSDAAGFLISNPSNRRKRKRLYLWGALAVIVVTGVGIW
                     AAPVRPDRRQIRTEEARRGNLVVTVTATGNLEPTNQVEVGVEVSGTIATVEVDYNDRV
                     ARDQVLARLDPTKFKAQVVKSRAALQSARARILQAQATVDESRAQLGRLTRLRVTSKG
                     TDPSQLDIDAADAALKRALADRASAKADVAEANANLESSEIDLAKTVIHSPVDGIVLV
                     RSVEPGQTVAASLQTPILFVLAEDLTKMELHVSVDEADVGRVRPGQRATFMVDAYPDR
                     TFSAEIADVHFGAQEVEGVITYEAVLNVDNGDLSLRPGMTATATIVVEELRDELLIPN
                     SALRFSPSLRKEAEPDAGLLGRLFPRPSRSPPKNRREQGSAKKEPRVWIVRDGEPVAI
                     PITTGATDGIMTELVQGDVEPGMPLIVEMTLE"
     gene            39081..39800
                     /locus_tag="VFG22_04825"
     CDS             39081..39800
                     /locus_tag="VFG22_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6415595.1"
                     /translation="MPAQEHTQTPLIRLEGVTRVYGTGDAATYALRGIDVQIGRGEFV
                     AVMGSSGSGKSTCLNILGCLDTPTAGSYLFRGIEVGNLGRNQRALLRRRYFGFVFQGY
                     NLLSRTSALENVELPLVYRGIARAERRRLASQALAAVGLQGRETHSPSELSGGQQQRV
                     AIARALVTEPDVLLADEPTGNLDTARSLEIMDLMTALNRERGITIIIVTHESAMAAYA
                     DRIINFVDGEVESVVHRGEVA"
     gene            39800..41005
                     /locus_tag="VFG22_04830"
     CDS             39800..41005
                     /locus_tag="VFG22_04830"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6415596.1"
                     /translation="MVYNAFRLALNEIRRNVMRSSLTILGVVIGVAAVITMVTIGGGA
                     TVQVTEQIASLGTNLLIVTPGQRMGMGQRAAAPPFQMDDVEAIGREIGTVAAIAPISS
                     QPMTAVFSNANWSTTVMGTDNQYFKVGNWALETGREFTESELRAGSAVCILGATVRRE
                     LFGNQDPSGSKIRLGKLSCVVIGTLLSKGQSTIGRDRDDAVLVPLRTFQRRLGGNQDV
                     GLIQVSVQNGASTESAEEDISELMRKRRHISASEDDDFTVMDLKEITKMLAGTTQVLT
                     ALLGAVAAVSLLVGGIGIMNIMLVSVTERTREIGIRLAVGALEREVLTQFLVEAVVLS
                     SFGGGVGVILALVVSILAAKALSVPFVFNLGIVIVGFLFSVGVGIVFGYFPARKAARM
                     DPIEALRHE"
     gene            complement(41106..41285)
                     /locus_tag="VFG22_04835"
     CDS             complement(41106..41285)
                     /locus_tag="VFG22_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415597.1"
                     /translation="MDQIVILLLLLFVLLRQAKNRTRSAGAMDPAVTPLDNDTWPDYV
                     RQVALLAHRAPGEGL"
     gene            41393..42388
                     /locus_tag="VFG22_04840"
     CDS             41393..42388
                     /locus_tag="VFG22_04840"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6415598.1"
                     /translation="MTRIISWTLGFLAVALIVLVAAVELKNRSGVRKATTEITAYQPP
                     PLSDFGATRTLTILPLIDWHASRSGLRTEMGVSYLIETDEHRVLFDVGQNTDHEFPSP
                     LERNMKELGIELESVDRMFISHNHFDHVGGKANQKNQTFSVGLEQVPLPDVLAFVPIP
                     MAYPGLRPIHAREPTKVGVGLASTGTIPRQLVFGWIEEQALVVQVEGRGGVLIVGCGH
                     QTIPRLLERYDEVFSEPLYGVVGGLHYPVPEGRLNMMGVNVQRRLASGDGMLAPLTMT
                     DVEAELDALRERHLGVIGVGGHDSSDEVITMFADAFGDAYRHVRVGEPIVIASEK"
     gene            42565..44490
                     /locus_tag="VFG22_04845"
     CDS             42565..44490
                     /locus_tag="VFG22_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415599.1"
                     /translation="MNSQLLKSITLALSLACLASMGCSSDASSTDQQVPEEVTSWEPE
                     GARTVGEYAYDTVPVPNAFHTMHAGPNNTDNVWVATAPQLELDWVSETSFYVPEGPTF
                     DNEGNLYFSPLFPQIDVSLVSLDAKTGERNWVVPGDGSNAGSGAVLILNDPANPGQQI
                     VYHATYTEAMAIKPDGTILWQTPTGLSLPPVVQGERSPTHSFGFNYHPRTDSLIGVTL
                     GGDVFAFNRVTGESVAPVIQIPGAPALSQELALPQFVVDASNDLTDAVFGTTPSGLSF
                     YSVIVDVIFGGGSKVTNYFAIDPNTSVVYVAATAPDEDDGTVDGVSELGAIYALDLVD
                     DGSGGLELRVLHSASFEGGTGSTPSVSEDGRRVYVSDNIGNVIAYDSELNEIWRFDLG
                     APVAASIAVSPDNAELYAVTQEDVFKLIDEGENARLDWMATLEAFEDDPEIEVVFQAL
                     TPSITANGIAVSVGGGKLLAGRGIMLRVGVGLLDRVTGELRSFTEGREESIAVTSIGP
                     DGGIYTASSPVRRVSGKALYPDLTKDIIGGISRYKPVRNDLLAKDASCAAGTRARNAA
                     TIADAAPRSAAEDMRQIQVLIDQIRAAITRAVADGDLDQATADALESDLDGAEAHLTM
                     DGLSTVASDLLRVCNAI"
     gene            complement(44537..45211)
                     /locus_tag="VFG22_04850"
     CDS             complement(44537..45211)
                     /locus_tag="VFG22_04850"
                     /inference="COORDINATES: protein motif:HMM:NF025594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4336 domain-containing protein"
                     /protein_id="HET6415600.1"
                     /translation="MQQLAAGIWSQSAPQSFYGLHVGTRMTVVQLGDGQLLVHSPLAM
                     TPDLKAEVDALGPVRHIIAPNIYHHLYAGDWKAAYPGALLHGARGLAKRRKDLVIDRE
                     LAGETFPDWREQLDTALLEGTMLNEMVFLHRPSRSLIVADLIENFETSPHWPTRAYLK
                     LGGIHGKPGLSRPLRMIFRDKKRARRALDRILSWDFDRIVLAHGDILESGGNETLREA
                     YTWLKG"
     gene            45300..45815
                     /locus_tag="VFG22_04855"
     CDS             45300..45815
                     /locus_tag="VFG22_04855"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET6415601.1"
                     /translation="MANPTATLETSLGNVEIELFADLMPTTAGNFIELAKSGFYDGLH
                     FHRVINGFMIQFGCPHSRDPKSPRAGTGDSPNGTIQDEHPDNAKLSNEPGTLSMANTG
                     RPNSGSCQFFINTVHNAYLDWFSPGPSKHPVFGKVVSGMDVIKKIETTPTGAGDRPAT
                     PVQMIKVTVHE"
     gene            45884..46567
                     /locus_tag="VFG22_04860"
     CDS             45884..46567
                     /locus_tag="VFG22_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415602.1"
                     /translation="MTSLRILLFCLAASLGLSACTDEVPPPMNGTGGRGGTAGSGGIA
                     GSGGSAGSAGSAGLGGSAGSAGSGGTAGSPGSAGSGGAAGSGGSAGSGGTGGAAGGMG
                     GGGMGGALSGACINQDDLAALADLQPSNAREVAADCGIECTDLIAFEQAFTTCASDCV
                     ERMVTGLSSGCATCYGELAWCSRLLCLTPCASASCLPVCQDCNGYDLCLAELEACTGR
                     VSVDCTDPT"
     gene            complement(46579..47061)
                     /locus_tag="VFG22_04865"
     CDS             complement(46579..47061)
                     /locus_tag="VFG22_04865"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET6415603.1"
                     /translation="MVEAKWQDLEAIKRLKYKYQRCLDTKEWEKLRDCFTEDAKAAYS
                     SGKFSFDGRDAILRFLEGAMGADSFHSSHVVSQPEIDFVDERTATGRWCLQDYVIDTS
                     FDMIIHGAAFYEDEYRKGDDGVWRIAKTGYTRTYEQMVPRKSIEGLTLTDSRWQPKGK
                     "
     gene            complement(47112..48248)
                     /locus_tag="VFG22_04870"
     CDS             complement(47112..48248)
                     /locus_tag="VFG22_04870"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HET6415604.1"
                     /translation="MDFELDQDQQMLAKTVADFAKNESPVERFRKLRDDDVGYDPAVW
                     KKMGELGWLGVPFAESMGGYGGSFVECAIVLENLATTLVPEPYLASVVLGGMTLARAG
                     DAAQQEAYLGPMIEGESTLALAHSEAETRYDVRSIETSATPSSKGYVLSGEKVWVLNG
                     HRADHLIVSAKAPGGVTLFVVPRGANGVKVTVAKTIDGQKAAFVQFDGVAVNEEARLG
                     AEGSGAAVLERTMDYGAAAAVAAGVGIANTMLQMTVDYLGTREQFGVKIGSFQALQHR
                     AVDMYAETELLRSISMASMVRADDDDDAARRADISAAKYQLATGGVWVAQQSLQLHGG
                     IGVTDEHDIGLYFKRMYALNALFGDEEHHVARFASDPSFLESGL"
     gene            complement(48280..49458)
                     /locus_tag="VFG22_04875"
     CDS             complement(48280..49458)
                     /locus_tag="VFG22_04875"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6415605.1"
                     /translation="MDLTFNEEEEAFRHQVRAWIQEAMPEDMRQKARGAGGFKPEDSK
                     IWHRILHAKGWIAPHWPKEVGGTGWNAARRFIFNEEMELAGAPPLSVFGLSMVGPLII
                     SFGTDAQKKRFLPKILSAEELWCQGYSEPGSGSDLASLRTTAEDAGDHFIVNGQKTWT
                     TYAQYADWIFCLVRTDPSAKKQAGITFLLIDLKSPGVEVKPMLTLGHTPAFCDTFFDN
                     VKVPKENVVGGINQGWTMAKALLGHERTMLAMIGPSRNVLQKIKRIAATKKIDGRPML
                     EDPIWRAKIARLEMRFRAHLMVSYRALADQQKGKMPGPESSILKLVGTKLIQELTELA
                     MEIMGESSLTWNNEGVCPPIEESIGPYFCYERAATIYGGSNEIQRNVIAKMILQLPSS
                     "
     gene            49548..50309
                     /locus_tag="VFG22_04880"
     CDS             49548..50309
                     /locus_tag="VFG22_04880"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET6415606.1"
                     /translation="MTIELAFLLFALVVVTAYAVQTATGFGATLVSVTIGAQLMGIEE
                     VIRLMVPLSFLQTGYIAIRHRDGIDWSLLLKRILPLMAVGMSLAFLLLTRVGGPWLGL
                     AFGFIVLGLSGRDLYLLRAAGTAVAKPVARAASVAAILSAGVIHGIYASGGPMLVYAV
                     GREGLSKKAFRSTISMVWIALNLVLVARFLLAGDYDRKVALDVLMLVPAVPLGILLGE
                     WVHHRVDERAFKMAVLLLLIVAAVSLIVRYSAQLL"
     gene            50325..>51428
                     /locus_tag="VFG22_04885"
     CDS             50325..>51428
                     /locus_tag="VFG22_04885"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET6415607.1"
                     /translation="MYDLKIVGGTLVDGTGTERRTGDVGIKDGRIVDVGRCDEGAARK
                     VDAAGAIVAPGFVDIHTHYDGQISWDEELAPSSIHGVTTCAMGSCGVGFAPVRPADHA
                     KLVELMEGVEDIPGSALSEGISWGWESFPEYMETLDRMPHTIDFLAHVPHDALRVYVM
                     GERAVHDEAANEDDIAGMRRLLREALEAGAIGFSTGRSDNHRSAVGDWTPASEADGKE
                     LAGIAKAFDGLSYGVLQAVSDFNVLHGDDHFDAEWGVLEQMLGAAGGRPMSVSTMQRD
                     HSPEQWKWILDKASKATEKGFEVRCQVGARGIGVMLGLQATFHPFMGFPSYKAISQLP
                     LAERVKAMRDPGLKARMIAEKSEPVAGDGSMLPP"
BASE COUNT         9961 a        15836 c        15753 g         9878 t
ORIGIN      
        1 gcccgctcgg ggatgcttca tgtagtagag ctcgaagaag ctcgcattgc ggccgtagtc
       61 gctttcctgc gcggtgaaga ccgcgccgta ggatccgaga cgctgactca tcatggtgat
      121 gccagcgagt cctagacgct ccacttctgt atcgccgacc cggatgcgct gggtttccca
      181 tcggtcaccg tcgctctcga tgttgatgtc gcggtgccgg taggttcgcc atggatgcac
      241 cggaaccaag aaaccgtgcc ccgcatcacc atagcgggac tgcaactcac ggcgaatgtg
      301 ccccgtaaac aaatcacttg ccacgtgcga agcgccaaag aaaagcagtc gcgccttgcc
      361 ctctcccgat tcggcccgct gaagagctcg atgcagagcc agcatcgact ttccgtcgcg
      421 gtcctcgatc gcgatatcga ggcccaatcc atcactcggc ggcggctgca ggagcttggg
      481 ccccatccgt tgccccgcgc gaaccgcgtc gagcacaccc gtttcgtcaa tcgctgcagg
      541 cgagcgcgga gacgcgtccg ccgagctcgt tgaagcgcca taggcaagcg tccagacgct
      601 tgtcatcgcc accccagcga gcacgatcgc caacggcgag ccccatagat tcccgagccg
      661 cacgccctca caatggcgat ccggctcgat ccagtcaaat tgacgtgaaa gcccgaccga
      721 agtcggccat gatgcgcgcc atgaagtcgc gcgagctgct attgaaggcg gcaaaattcg
      781 acgttgctcg cctgcggttc gaaggccctg acggcaagga gatcgtccgc gacctggtcg
      841 aacaccccgg tgcagcggtc atcctacccc agttggacga cggacgtatc gtcttgatac
      901 gcaatctccg tcgaacggtc ggcaaaatac tctgggagct tccagcaggc accctcgatc
      961 cgggcgagtc cccggaggtc tgcgcagcgc gcgagctcga ggaggaaacc ggctaccgcg
     1021 ccgggaccct cgcaccgctc acggcgttct tcgcctcgcc cggcatactc aatgaacgca
     1081 tgcacggctt cttggcgacc gacctcgtcc ccacggcgca gaatctcgac cagggcgaag
     1141 agattcaagt cttcccgatt ccgcagtggc aggtgcgaga catgctcaag gatgggcaca
     1201 tcgaggacgc caagaccatt gcccttctgc tttattggat gcatctctac tcaccgtaac
     1261 ctgccgagcg tcgacttcga tggatctgct gagccgcatt caagacaact ggatcgtact
     1321 gctgatccct ttgatcagcg cgctggtggg ctggttcacg aatgtcgttg cgatcaagat
     1381 gatgttctat ccagtcgagt tcttggggat tccaccctac ttgggctggc aaggcgtgat
     1441 tcctgcgaac gcggttcgac tcgcgaaagt atcgaacaag ctcatcacgc aaaagctgct
     1501 ctcccttcga gagctcttcg atgaatcgtt caacgccgaa tctttcgctg gaaagctggg
     1561 cgccgtgatc gacgacgtca ccgagcaggt gatcgacgag gttgccaaca agcacgccaa
     1621 ggcaatgtgg gacaacgccg gcgatttcat gcagaacaag gttcgagaga aggtgcgctc
     1681 cgaggtggtc gaagtctccc gggcgatcgc catggacatg gccgatgaca tcgacgagat
     1741 catggacatc gaacagaccg ttctcgaagc catggaacgt cacaagggcc tcatgggcga
     1801 gatgttctac gaagtgggtc gcctggagtt caagttcatc gaacgctcgg gcctatactt
     1861 cggatttctg ttcggggtct tccaaatgat cgtctgggtc ctctatcccg caccctggat
     1921 tctgcctgcg gcgggcttct tcgtcggcta cctcacgaat tggattgcca tcaagttggt
     1981 cttctcgcca cgcgagccca cgaagatcgg cccgatcacc atccagggtt tgtttcacaa
     2041 gcgtcagaac gaagtcgcgg aggctttcgg tcgcacggtg gccacgcgcg tcctgaacgc
     2101 ggacaacatc gtgaccaccg tgatggagag cgaaggagcc aagcgcatga acgagatcgt
     2161 cgcgcaccgc attggcgagc tgatctcgaa gtacgaggcg catccgatgg cgaagctcgt
     2221 ggtaccagaa gcgagccaac ccgcgcttcg ggcagaggtc ctcgaacgaa ttcaaacgga
     2281 atggccgaaa ccgggggggt tctttcacac cttcgcgggc gaggccgtcg acctccaggg
     2341 cgagctcgaa cgtcgaatga aggccctcga tcgcgagagt tacgagggag tgctgaggcc
     2401 tgctttccag caagatgagt ggaaactgat tgcggctggg gccgtgctgg gcaccatcgc
     2461 gggcgtcctt cagctcgtgt acatcttcgg tgacgcaatg gagcgattga gccgctgagc
     2521 ccgccggacc tggccgatcg ctcgtccaac ggctaaacac tgggttgtgg aacaaaccgc
     2581 gaccctgctc gtctgctgcc ctgaccgtaa gggcctcgtc gctgcgctgg cgcaggtgct
     2641 ctatgggcac ggcgcgaaca tcctcgatgc tgaccagcac accgatccgg tggccggtca
     2701 gttttttcag cgcatcaagt tcgatctctc cgagcttcac accgacagaa cgagcctcga
     2761 gaccgcgatt tcagaggtag ctcagcgctt ttcgatgaat tggcgcatcg ctgacggccg
     2821 gagtcgctcg cgaaccgccg tcttcgtttc gaagtacgac cactgtctgt acgacttgct
     2881 cttgcggcag cgcgccggtg agctctcgac ggacattccg ttgatcatca gcaatcatcc
     2941 agacctcgag ccggtcgcca aacagttcaa tgtccccttt cgtcatctgc ccatcgccaa
     3001 gggcgcgaaa cgcgcgcaag aggaagaagc tttggcgctg ctcgaggccg agcgcatcga
     3061 gctcgtcgtt ctcgcacgat acatgcagat cctgaccgac gattttttga aagcgttttc
     3121 gggacaggtc atcaacattc atcattcgtt tctccccgcg ttcatgggaa gcaagccgta
     3181 ccaccgggct ttccagcgag gcgtcaagct cattggcgcc accgcacact atgcgacggc
     3241 tgacctggac gagggaccga tcatcgaaca agacgtggtg agatgcactc accgtgattc
     3301 ggtccaggac ctcgtccgca aaggccgaga cctcgagaaa gtcgtgctag cgcgcgccgt
     3361 gcgctggcac cttgacgatc gaatcctcgt ctacgacaac aagaccgtcg tctttagcta
     3421 aggagtccca atgtctgaac gagtgcagct cagtatcgaa gagcacgtcg cgacggttcg
     3481 tctctcgagg cccgagaaac gcaacgccct cgatgcagag atgttcgaag ggctcattgg
     3541 cgtcgggaaa cgcataatgg aagacaaacg ggttcgtgca gtcgtcttgc acggcgaagg
     3601 caaagccttc tgcgcaggac tcgacttcgg ctcgttccta ggctctcccc agctgatgca
     3661 gaaattgctc tcccgcagcg aagaaagccc cgcgaacatg gcgcagcgcg ctggttggat
     3721 ttggcaggag gtccaggttc ccgtcatcgc cgcgatccac ggattcgctt ttggcggtgg
     3781 gctacagatc gccctcggcg ctgacctacg ttatgtaacc gccgacgctg agctctcggt
     3841 gatggaaatc aagtggggat tgatccccga catgagcatc acgcagactt tgctacacct
     3901 agttccgctc gacgtggcga aggagctgac cttcacgggc aggatcatct ccgggaccga
     3961 agccctcggc ctcggcctcg ccacgaagat ttcggtcgac ccgctcgctg atgccctcga
     4021 aaccgccagc ttgatcgcga gtaaatctcc gcatgccatc cgagcgggca aggctcttct
     4081 tcgcgatgcg ccggggatgt cgagggccga tgcactgaag ctcgaaacgg cccttcaagt
     4141 gaccctgctc ggcagtccca accaaacgga agccgtgcag gcgaacatga tgaaacggaa
     4201 gcccgacttc aaagatcccg aatgacctga gatcaggttg cgtcgggtgg tcgcgagacc
     4261 cgcggatggt actggcagca atcggctgga cagacatcgt gcgacgggcc gcgattgcca
     4321 gccgcttgtc gagtgctgac tttgcccagg cgctcttcga tcagctcgcg aatcattcga
     4381 acgaacgcgg gatgggttcc cacggtggga gcgcgtgtca gcgtgatccc gagatcgttg
     4441 gcgtagtccc gtgcctcgtg gtcgaggtcc cagaccactt ccatgtgatc cgataggaaa
     4501 ccgattggcg cgacgacgac gtccttgacg ttgatctgcg cgagcacgtc gaggtgttcg
     4561 aggatgtcag gaccgagcca ggggatgtgc ggcggtccac tacggctctg atacaccaac
     4621 tgccagcgtt cgtgcctcgc gccatcggcg acgagacgcg ccgtctcttg aagctgctcg
     4681 gtgtagtcgc agttctccgc catcgagatc ggaatgctgt gtgcgctgaa cgccacccga
     4741 gcgcattcac gacgctcttt gggtagcgtc tccaaggctt cgagaagtcc atcgacgttc
     4801 gcctcgatga aacccgggtg attgtagaac gcgcgcagct tgtcgacgat gggtgcgcct
     4861 tcgcctattt cttctcgtgc tttctggatg ttctcacggt actgccggca accgctgtag
     4921 ctactgtagg ccgaggtgaa gaatgccagt gcccgcttct ttccgtcatc gcgcatctgg
     4981 cgaagcgcat cgatgatgaa agggtcccaa ttccgattgc cccagtagac cgggagatcg
     5041 atctggctct cctcgagctc tttttcgagc gccgcgatca acgctcgatt ctgatcgttc
     5101 agcgggctct tgccctcgaa atgattgtag tgcgccgcaa cttccttgag acgatcaggg
     5161 ggaacgttct tgcccctgac tacgttctcg agaaacggca tgacatcggc acgcttctca
     5221 ggcccgccga aggacaccag cagcacactg tcgtactcca aatccgaatc gcccacgacc
     5281 gaactctcgc gcctaaaacc cccggtggca agtcaagagt cttgccaagg aatccgagct
     5341 gtgctctagg ttcgtcgtgc gagttacaat cctgggaagc ggcagcgaag gaaacgcgct
     5401 acttctggag tcgggggcaa cttgcatcct ggtcgatgca ggcctctcgt accgcaagct
     5461 cgttcagcgg tttgccgcga tcgggcgcgg cgttccctcg aatgtcgctg ccgtcatcat
     5521 cacccacagc cacggcgatc acgcagccca tgcatcgacc tacgcgtcgc aattcgcttg
     5581 tccaattcgg gctagtgaag caacgttgaa gtcgattcgc ttgcgcccgt cggtcgcatc
     5641 tgaaactttc gacgtgggtc ggtgcatcag catcggggag atctcgatcc aatcgcgcgc
     5701 gattccgcat gacgcgcccc aggtcgcact tcggttcgag aacaagcaga cctcggtcgg
     5761 cttggtgacg gacctcggac acgttcccaa gggactggac cgctttctcg acgggtgcga
     5821 gacgctcttg ctcgaaagca accacgaccg ggacctgctt gctgaaggcc cctaccccga
     5881 cgtgctcaag cggcgggtcg gagggtcgct cggccacctc tcgaacggcc aggcggccga
     5941 cctcttggcg gagctccggc gcgctccccc acgcgtcgtc ctcatgcacc tctccgaaac
     6001 caacaactcc acgcgcttgg ctcgcagctc cgcggaagcc gcgctgggac atcagggcac
     6061 gaagcttctg gttgcgcatc agcaaaggcc gttggagctc gacccggttc gcgaggctca
     6121 aatgcagctg ggtctctagc gggtcggtga tgcgcgcctg gtacgacgag atcttcgagg
     6181 atcacacccg actcgggttg cgagtcaaag agaccctgta ctccggagcc agcaagcacc
     6241 aagaaatcga ggtcctcgac acgatcggtt ttggcagagt tctggtgctc gatggggtgt
     6301 tcatgaccag tgagctcgat gagtttctgt accacgagct gctcgtgcat cctgcgatga
     6361 cgaccgcccc agagatcaag cgagtgctgg tgatcggcgg aggcgacggt ggcacggttc
     6421 gtgaggtgct gcgccattcg ggcgtagagc gctgtgtcat gatcgaaatc gaccccatgg
     6481 tcgtcgaggt atcgaagcgg tacttgtcag aaatcggtac cgcgtggcac gaccctcgcc
     6541 tcgacgtccg attcatcgat ggcgtcgagt acgtgaagct gagcgacgat gagccgtacg
     6601 acgtcatctt gttggacggg acagacccgg tcggtccagg tgcggccctc ttcgacgaga
     6661 gcttctacgc cgggtgcaag cgcatgctcg tgccgcgagg ggtgatggct ctgcagagcg
     6721 agtcgccctt catgatgatg gacgtcttcg tcgcaaccca gaacaagcta cgaaagctct
     6781 tctcgcacgt gcacccgtac ctcgggccgg tgccactcta cggaaccggc acctggagct
     6841 ggacctggtg ctccgatatg ggccgaccat tggaagcgat cacccatcgc caggaggccg
     6901 tcgtgcaagg ctccaaggcc tacaacgcgg acctgcacca agcggcattc gccctgccga
     6961 actacgtgaa gagagctctc gaccccgagg atgtaagcgc gtgacgctca tcgacgttcc
     7021 gctcgcgggg cagggagtcg tcaccagcgc agaaccagct ttccgaacgt gtcgtttgcg
     7081 tccatccgcc ggtgcgcggc ctggatttcc gtcatgggca ggatgtcatc gatgattggt
     7141 ttgagctcgc ctgtttcgaa acgcccaagc atctctccgg tgaaggattt cgtgagctcg
     7201 gctttctctt cggctgagcg gctgcgaagg accgagccct ggatcgaggc tcgtttcgca
     7261 agcagcgcgc cgagtgcaag ctcggccttc actccgccca tcaagccgat gacgatgatc
     7321 cggcccttct tggcgatcac tttgaggttc tgcgccaaat aggccgcacc gaccgtgtcg
     7381 aggatgacat ccgcgccgtc ggggatcgcg gcgagcactt cgtcggcgaa attcccatcc
     7441 tcgaccaaga ccgcatgggt caggccgagc ttcgagcatc gatctagttt gtgcgcgctc
     7501 cgagacgtgc ccaccgaaat tgccccgagc gcgctcgcga tttgaatgcc ggccgtcccg
     7561 acgccgctcg ccacggcgtg aatgagcacg gactttcctg cgcctagacc tccttgaagg
     7621 accatggaat cgtaggcagt gaggaacact tcaggcacgg ccgccgcctg ctccagcgaa
     7681 aaactcttcg gcacaggcat cagctcggca gactcggtca gcaagtgggt cgccatagcg
     7741 cctccgccaa cgatgcccat cacccggtct ccgggccgcc aagcggtaac agactcgcca
     7801 accgattcga tcacgcccgc gaactcgaga ccaggtacgt cgacaggcac acccggaggg
     7861 ggcggataga acccacgccg ttgaaggcag tcggcacggt tcagccccga ggccgcgacc
     7921 tcgacgagga cctgcgaagg tccggggctt ggcagctcga tctcgccgag ctcgagcacc
     7981 tcgggtccgc cgggctcccg aatgcgaatc gctcttgcgg tgaccatggg gcgaagccta
     8041 acgcgcccgc ggcgcccgtc cagctcagcg tctcatgcta gcttctcggt gatgaatgcc
     8101 aacgtcgggc gttttctgca ccattgggcg ctgcaagacc ccgagcgcac cggcatcttg
     8161 gactcaggcc caggcgagat ccggtacagc tacggagagt tggaccgcga ggcacgccgc
     8221 gtggccgggc atctgcggag tcggggcctc ggtttgcacg atcgcgttgc gatctgcacc
     8281 gggaacggtc tgcatttcgt cgcagcctgg tttggtgcag cctatgcggg ctgtacgact
     8341 ctccctatcc cgttcatgtc gacggcgcac gagatcgcgt tccgactgca gcacgcgcgc
     8401 tgcaaggccc ttctggcaga cgcggagcga tccgcagtgg cgaaggagag tcaactccgc
     8461 gcaggcggtt tctgtgcgct tctctcgatc gaagaggcgc tcacgggcaa cgtctccgaa
     8521 gaggctccct gccatgctcc ggcagatgcg tttgccatgc tgctctatac gtctgggacc
     8581 acgggcgccg caaagggcgt gtgcatcacg cacggatctc tcggcgcgca tacccgcgcg
     8641 cttgcggagc aaactctcga gttgacctcg gaggatcgaa tcctcggcac ccttccgctc
     8701 acgcactcct atggaattcg cacgaccctg ctcgcgccct tcttcgcggg cgcaagcaca
     8761 gtcttcgttc cgaggttctc tgctgccctc acactcgagc tatgcacgag gcaccgcgtc
     8821 acctggcttc cggcggtgcc cacgatgctc gtggcctggg ccaacgagcc gagcgggcca
     8881 gtccctcccg ccctccgctg gtgcctgagc gccggtgcgc cgttgctaga aaacgtccga
     8941 cgccgcgccg aagatcgcct cggtgcgccc gttcgtcagg gctacggtct cacggaagcc
     9001 aacctcgcgg cgatcaacgc cccacccgat cgcttgcttg aaggctcgtc cggcaagccg
     9061 gttccagggg tcgagatccg aatcgctggc gactccgagg aagagctccc gcctggcgag
     9121 cgcggcgagg ttctgattcg gggccaaaac gtgatggcag gctatctcga tgacaagatc
     9181 gcaagcgccc atgtgatgcg cggcgggtgg ttgcacacgg gggatgtcgg cttcgtcgac
     9241 ggcgagggac gactcaccat cgtcgaccgt agcaaagatc tcattctccg cggcggcgct
     9301 agcatctacc cgtcggagtt ggagaacgtg ctcgctcatc actcggcaat tgacgacgtc
     9361 gcggtggttg gagttcccca cgactactac ggtgaagagg tcatcgccgt cgtcgttcga
     9421 agcatgaaag tcacgactcg cgagctcgac gcatgggtgc gagagcgcct ggcgccctac
     9481 aaagtaccca gaggctacgc gttcatcgaa tcgattccag aaggtccgag cggtaagagg
     9541 atgaaacgcc gtttgcggga ccagctcgtc gcgggtgaga tcgagatcga gcgcctagcg
     9601 actcacggga cggctcacta gctcgaatac gccgatcgtc actgcggctc cgagtagcag
     9661 tccgacccag ggcccgtacc atccggcggc gaccggcaac tcggcgcaga gcaggctgac
     9721 ggcaccgatg aacaacgcat atggcagttg cgtacgaacg tggtccatgt gatcgcagct
     9781 cgtcgccatc gagctgagca ccgtggtgtc tgagatcggg gagcagtggt ccccccagac
     9841 cgaccccgcg aggatcgacg atacggtgcc caagaggatg gtttcgtttc caggagcaat
     9901 gcggtgtgca agtggaacga ccagcggaaa gagaatcgcc atcgtgcccc aggatgtgcc
     9961 tgtagcgaag ctgaccaagg ccgccagaac gaaaaccgcc gcaggaatcc aagtcggagc
    10021 gatccaatct ccgaagaccg caatcaggaa cgatgcggta tcgaggtccc ggcagaccgc
    10081 actgagggac caggccaaga cgaggatcac ggcggcgaaa agcatcgaac gcatcccgga
    10141 catccaagcc tcgatcgatt ccccgaagct gagcgcgcgg gttcccacgg agagagccaa
    10201 agccgaaagg caccctgctc cagtcgccca gaggatggcc gcgctcgagc ttgcttcgcc
    10261 gacgatcgcg cgaaggctcg gctcgagacc cgccgacaga accttcgcac ggccgtctac
    10321 gaagatcccg agggcagcca ccaggacgag caccagaacc gggatcagcg cattcatggg
    10381 tcgacctgga acctcatcgg gcaccaactc ttcatgaaag tccgaagctg gctgagcgcc
    10441 aggtcggaga accgatccgc tttcgagggc gcggcgttct gcccgcaaca tcggcccgaa
    10501 gtcgcgcccg cgcaaaccga tccaggcgac gaataacaac atgagaatgg gatagaaccg
    10561 aaacggaatc gtttcgatga acgtgacgta cgcatcacgc tcgatgccga gttgcgtgag
    10621 ttgctcgccg atgtacccga cctccacacc gatccaggac gagatcagcg ccaagctcgc
    10681 cacgggcgcg gccgtggcat cgacgaggaa cgcgagcttc tcgcgactga tgcgaaggcg
    10741 atcggtcacc ggtcgcatcg atgagccgat caacaacgag ttggcgtagt catcgaaaaa
    10801 gatcaacagg ccgagaaacc aggtcgtcaa ttgcgcgcga cggcgactcg tcgaatcggt
    10861 acccacccag gatgcgagcc ccttcgcgcc accactttgc gtcatcaagc cgagcatccc
    10921 cccgagcagc aggctgaata ccagtatcga cgcatggcct ggatcggcaa tcgcgtggac
    10981 gatgtaggtg tcgagcagcc tagcgaaaga agcgaggggt gcgagatccg agagtaggaa
    11041 cgctccgagc cagaccccga gcaccaaggc gaccaaaaca tgacgaaaca gcagggcgag
    11101 agcgatcgcg agaagaggtg gcagtatgga ggtccaagcc ggaagggcag attctccacc
    11161 tcccgcagct acctccacgc tcgcgagagg tatagtattc gaggaagcga tctgaatgag
    11221 ccgaggatcg tcgccgtcag agctcgtagc gccgactttg gcggcaccga acccaacgac
    11281 aaccatcaga accaacagga ccgctcgcat ggacagagtc agacatagac aggaacgcag
    11341 ccgctggtca attttgaccc ctgcactggc gctgctcttc gtcgcactgg ctcctttggc
    11401 agcgccgacc cgcgccctgt ccagcaacga tgagctcacg acggaggaca aaaagcaatt
    11461 ggctgctggt gagctggtca cgaagcaaaa gcacgagcag cgaggcgcat acaagctgat
    11521 cggcgggcaa agctggcaga tcatcaacgt cccggtcgat gtcgcatggc aagcaattgc
    11581 ggacctgtcc ggttacaaga atttcattcc gctggcgact gaatcggacg tcaagcatca
    11641 ggcaggcgag gagaccgact tggcgattcg tcagcagtgg ggtcccatcg acgtgcacta
    11701 cgtgcttcag gctaccctcg agcccgagcg ccgcgcgatg gttttccgcg tggatcattc
    11761 caaggaacac gatatccgag caggctgggg tttcatgcgc gttcgcccgt tcaaaggaga
    11821 tcgcactctg gtttcgtttg gggcactcgt ggacatcggc gacggggtct tcgtttcggt
    11881 gatccggccg gcggtgcaaa aagacctcct gcgaattccc tatttcttca agcgccacct
    11941 ggaaggcatc gaccccaagt ccgaagcgga gctcccgcga agaaaccaga gcaactagat
    12001 cagtttcttg atccagctca gcttgcgggc cgagtacggc ggaaacaaga gcgctggttc
    12061 ccaaagcgcc cgcttcttga ggaccgctcg tctgtgggaa aacgtttcga acgaatgacg
    12121 tccgtggtag gcgcccattc cgctttcccc cacgcctccg aatggaagct cgggtacccc
    12181 gaaatgcagc caaacgtgat tgatcgtggt gccacccgaa ctcgtggcgc tgagaacccg
    12241 gtcttcgacc tgcgagtcgc tggtgaacag atagagcgct agtggcttct cgcctcgatt
    12301 gacgaaggca agcgcctcgt cgatggcttg gaaggaaatc accggcagaa tgggcccgaa
    12361 gatctcctcc tgcatcagcg catcatgttc ctttgcgttt ttgacgatcg tcggtgcgat
    12421 gtagaggtct gccgggtccg attcaccgcc tacgacgacg tcggcgccct ggagaaaccc
    12481 agtcaagcgg gcgtggtgct cccggtcgat gatccgggcc aggtcggggc tctcgtgtgg
    12541 ctcgtcgccg tagaactcgc gtatcgccga caccatgcga ttgacgaggg catcgtgcac
    12601 ttgctcgtgc accaagacgt agtcgggcgc gatacaagtt tgcccggcat tgacgaactt
    12661 cccgtacacg atccgtcttg cggccaggtc caggttcgcc gtctcatcga cgacgcacgg
    12721 gctcttgcca cctagctcga gtgtgacggg cgtgaggttc ttggcagccg cttccatgac
    12781 gacgcggccg acctttgcac tgccggtgaa gaagatgtgg tcccagcgct ctcgcaagag
    12841 cgcggtcgtc tcccgcacgc cgccctcgat cacgcgtacg gccctggggt ccaaatacct
    12901 tggaatccat tgggcaagga acgccgagac attcgggcag agctcggagg gtttcaggac
    12961 cacgcagttc ccggcggcga gcgcgccaac caatggcaga cagagcaagt ggaaggggta
    13021 gttccaggca ccgatgatga gcaccacacc gagaggttcc cgcagcacga agctccgccc
    13081 tggcactgcc acgagcgagg tgcgcacacg ctgcggtttc atccaggacg cgagcttctt
    13141 gcgggtgagg cgaagctcgg aaaggatcat gccgatctcc gaagtgaacg cctcgagtgc
    13201 aggcttgcgt agatcagcgc gcagagcatc taggatgtct tgctctcgct cgatgaggag
    13261 atgctcgagg gcgtcgagct gcgacaggcg ccaggacagc ggcttgctcc ggcccgagtc
    13321 gaaatgggct cgaagcgcgc ccgaggctcc tgaggcctgg cgctgccgct ggctagaaga
    13381 ggtcgaaact gcgcttgttt gaggcatcgg cggcgctcct atgaggcatg gcctgagaaa
    13441 ttatcctgta attttcgata gttactgggc atgtgaaaca attccgaaac agctccgatc
    13501 tctgtatact cgtccgacgg gattcgtttt gccctacgga ggccctgatg cacagcagcg
    13561 aacgtacttg gaaacctaac gcgaaacctg ctccttggtg ggcgcatatt ctacgttggt
    13621 ggctgtagcg caggctaggc agcgagactg agcttcttcg ccggctcggc gcagtcttgg
    13681 cttgcttcga cttgacctcg gtcccgccga cgtccttctt cgaggacatg ctctgggacc
    13741 ccacgaatgt cccaaacgag acccacccag ctcagcatct tgagcacgta ataggtcaca
    13801 tcgatctccc accagtaaaa gccgttacgg cacgatgact ggtagtgatg gtggttgttg
    13861 tgccagccct ctccgagggt gagcagcgcg gtgacgatgt tgttgcggct ctggtcggtg
    13921 gtttcgaatc gcctcgtgcc ccagacgtgg gtgagcgagt tgatcaggaa cgtgttgtgc
    13981 caaagcagca ctgtgctccc gaagaaacct atgaagagcc ctggccaacc cgcgatgagg
    14041 aatgaaacga gagcgagagc cgctggagga acggcccagt acttgttgag ccagcgaagc
    14101 tccggatact tggcgaaatc tcggatcttg tcccagcggg tttcttcggt gccgtcgaag
    14161 atccacccta ggtgtgcata gacgaaaccg ttctgcaccg gagagtgcac gtctccaggt
    14221 tgatccgaaa gcttgtggtg cacacggtga tgcgaagccc accagagcac gcctttctga
    14281 gagctgctct gtgcgagcac cgcaaggaga aactgaaacg agcggcttgt cttatacgtg
    14341 cggtgggaaa agtaccggtg gtagccggcc gtgacacccc agaggcgtac gaacaagagc
    14401 acgaagcaga gaaccagcgc ctgccaggtc acacccgacc agaacgcgcc gagcaccgca
    14461 agatgtgaca gcacgaaaat cgtcgcccgc tgccactgct cgacgcggct gagcgaaacg
    14521 tgcggcgtcg ccgcctcgag agccctggtt tccatcaccg aaaactatcg cgggcacgcg
    14581 gattccaccg tcacggttcc gccccatttc gtcatagttt tgtaaaaagt ggacgatccg
    14641 gtcttctcag gcgacgaatt tgtagcccgc accccgcact gagaggaaat gtaccgggga
    14701 gctcgggtcc tgttcaaagc gtcggcgcag ccgtgagatg aagttgtcga cggtgcgggt
    14761 gttgccgtag ttacgtagtt tccagactct ctcgagaagc tcggttcggc tcagcacccg
    14821 accagcgttc tccgcaaagt aacgaaggag gtcgagctcc agttgagtga gctgaaccac
    14881 gtcaccggat acggtgactt catgggtgtc gaagttgatc tctgcaccac cgaaggagag
    14941 catggtcgcc ttcgattgag taccgctaac ctgttcccat cgctgccgac gaagcaccga
    15001 gcgcactctc gcgagcagct cacctagctc gaacggtttg accatgtagt cgtctgcgcc
    15061 tgcctcgaga ccgagaactc gatcctctgg ggatgagcgc gccgtgagca tgatcacggg
    15121 gacgaaattt cctgcttcgc ggaacttctt gcagagctcg aagccgttga catctgggag
    15181 catgacatcg agcacgatcg cgtcatagcc gctcggatcg agcaagcgtt gcccggcctc
    15241 cttggcgttc ccggcaacgt cgacgcgata gccttcgagc tccaggttga gcttgaggcc
    15301 cgcggccaaa tgttcttcgt cctcgacgat caagagacgc tgcgcttcgt tcctggtcat
    15361 gacgcgttca ccgcgtgagc aagtggcagc tctacgcgca tggtggtgcc ctgcccccgc
    15421 ccttcggaaa acgcgcgcac gtctccgccg agttgcttca ccagcgccca aacgacgaaa
    15481 agccccaagc ctgtaccccg acgctcgcgc acctcttgct cctcgacgcg atagaagcgg
    15541 tgaaaaaccc ttttgaggtt cttccggtcg attccgatgc cgccatcgcg cacctcgagc
    15601 gccatcttct tttccgagtc cctgcgcgcc acgactcgca catgcgccgg tcgcgccgag
    15661 tacttcaccg cgttgtcgat gaggttcttc acgacgattt cgagcgccgc gcggtcggtc
    15721 gtcacgatga gcgccgggtc gacgtcgatc tgaacttctt cgggatctaa cctgtgccgc
    15781 gcgcgaacga cctcggcaca ttgttccgtc agttggtgga gggacacatc gctgagattc
    15841 atcatgcctt gccgcgcata cgacagacgg ttggcttcga gcacatcgtc gatgaaggcg
    15901 cgaaggcgat cgacgtcttc gagcatcatg tgtcgcagtt tttcgcgggc ctcatcctcc
    15961 agcccttccc gtccgagagt ctccaagcag agctgcaagg atgcgagcgg gctcttcaac
    16021 tcatgtgtga ctgagtcgat gaaagtgtcc tgttttcgga cctcgatgat tccgcgggcg
    16081 agtgaaatca cgaacaagac cagaacgacg atgatcagaa cgaacgagag cgcacctagg
    16141 acgaggagcc agacgtcccc cgcgacgtct gctgagcggg tgatcttctg accgagaaga
    16201 aacgaccatc ccaccagcag cgccatgctc agcaaaacgg tgatcgcgcc caaagtgatg
    16261 gggatcgaaa gggaagagcg aaatctgcga cgacgtgcca tcagctcacc cgaaactctt
    16321 cactttcctc gcagcaaacg ccataggttg tctgtctgcc attgctgtgc cgccgaatga
    16381 tactcgaagc tacgctctcg ctttcgaaac gcccgggtat ggtagtgccg ccggccgttt
    16441 tggacgactg cgggcatgtc gtggtggagc atctcgtgga acacgacggc ttcgacgaaa
    16501 aaaccaggga cccagctctg gtcgagaacg ggatgaattc ggatcaatcg ttcctcgggc
    16561 aggtacgttc cgagttgcag ggaccgctgt cgacgctttg gttggacgcg tttgccccag
    16621 gtgatgcgga catcgagggc agatcccaaa tgtcggcggg ccaagtcgtc ggcgatcgcg
    16681 cgcaagtcat gatggcgccc tgcagacctc aaagtgtcct gaggtggccc cggagccacg
    16741 aaccgcaagc ggttgtcttc gacgaaacgg tcgatcgcgc ttgcggcctg ggaatcactg
    16801 tcgctcaagt agtcacccac ggcggccaaa atgtcctctt cggccgtgag gaacatgtgg
    16861 tggatgcgga cttcgagcct tccgcctttc gaacgagcgg tgagcatggt gcttcgattg
    16921 tcggtcacga cgagacgaat tggctgcccc acccgcgctg cgagcgcgcg gctccagtcg
    16981 gatgcgcggg catacgctcg gtcgagctcg tcctcgcggc gctcggggac gccacgctcc
    17041 gcgatcgtcg gcgatgcgcc ggcaaactcg aaggaaagtt gaagcacgcc atctggggct
    17101 agatgtttgc ggcatccttg tcaaccgtgc tttcggagtg gcgatccgca ggctagaatc
    17161 ttcgaggatg cgtgctgcca atcgggatcg cctcgaaggc ctgacagtct tgcaccggct
    17221 gccgagctcc gagcagcggg cggctgcctg gcgtcagggt atcgccactc tcgcgcgagc
    17281 ggtggtcgac gagcgccggc cggtgcccct cgaaggactc gacccgaacg ctctgctgca
    17341 gagtttggaa gtcgccgaca cggagggcct ggtcgagcaa ctcgattgga tttcggggcc
    17401 agctgcagcg gctgcgctct acgaggtcgc agctgccctt ccctcgagcg agatcaagcg
    17461 caagctaggc cgaatggttc tgcagcgtct acgtaaaggc gatgcggcga cgttcgtgtc
    17521 cgtagcaaca cagctggccc tcggatccca acgggcgctc actggaaaat cgattcgcgc
    17581 acgcgttgcc ctcgcgctga gccttccgat cggaacggtc aacaatgtcg acgccctcgc
    17641 tctagcgctc atctcgcgac aggacacgag tcgcgactgg ctgcgcacgc cggcaaccgg
    17701 atcccttcct tcccgacgtc tcgctgctcg cttgctcgag cgtgcagcgc gcgaagccgc
    17761 ccgccgagca gcggagggtg acgatactgg cgtgcgcgtc ttctgcacgg aggcggtccg
    17821 ggagccctgg ggccgcctgc tgggagatcg agaaaacttg gtgtggcggc atgttgcctc
    17881 ggccaggggc ttgcttgctt cgtccatgcc tatcttccgc gaagagatgc agcggcatct
    17941 caaccctgaa tttaccatca cggaatggcg acgcgcggcc acatccctgg ccgccagcat
    18001 cgcggtcgag cccgacgaag ggctgcgagc gtgccgagcc cttctcgaat cggtggtctt
    18061 tcaacaggac catggcatcg cggccgccat gatatcgggt cttccgcgag ccgcggaaag
    18121 ccaccccgtt gccgtcgaag ccttgctcga gcaactggtt cgagtgggtg ggctcgacgt
    18181 ggccgaggct ctggtcgagc tccgccgcga gcaagtccat ccgggattcg cagaggaggc
    18241 ggcaaggaag gccgagacgc atctgcgcaa ggccattgca caaggcggca acgaggatct
    18301 tgggcgcacg gcactcatga agtccctcgc cgacgagctc catggagcgc gccgctccag
    18361 cgaaccggcg cttcgcgaca tgctctctca ggcactgcag actttcgcca accaaggcgc
    18421 gtacgaagcc gctcgagaag cgccgtccat cctcgaagct gcaggccgga aggcgaagct
    18481 tctcgagaag ccgacggcgg gtgacccctc gagcagcatc gactcgtttc tcgcgctgcg
    18541 cgagctggac tcggccctct tcgagacctc tgcgttgaac gaccttctgc tcgtcggtga
    18601 ggatgacgct gggagtgcca ttcacgatga gcggctcggc gacctattcc aaaaagtcac
    18661 caattggctg atcatccggg agggcgatcc ccttcaagag ggagctcagg tcccacaatt
    18721 cacgacccgt ctgcgtcagc tgcgaacgat gctgcatctc gtcgatgcgg acggaccgag
    18781 cgtggattca cgaagagacc tcgttcggca acgacgcctg ctcacgcaac gcgtcctgct
    18841 caatcgagtc cggcacgatc cgaagagctc gcttcgacga acgttgtgcg cggcgacggc
    18901 gcgagcctgt gacgccttgg tacgcgagga cattgccgac gtgtcggacg tcgtcatagc
    18961 ggccgccgga aacgttcctg atctggacga cctcaaaacc atggcggaag cctcgatggt
    19021 ccccgaggtc gagagcgcgc tccgtgcgta tgcgaagctt cagcaggcga gccgcacggc
    19081 ccaacgagac cgccagggac tgatggcagc gaccgatgcg ttgaaagcga tcggcaatga
    19141 ccttccggtc gctcgttccg cccatatcga ggcatttcgt cgatcgcttt cgctcctagc
    19201 gagcggactt cgttccgcag cggcttcgag ctcgctcgcc gaagtggcgg agcgctcctc
    19261 ggggacgcca ctttcgaagc tcgaacgagg tgttgcaacg atggcgcaac tggtcagcgg
    19321 ctctcgacgg cggcttggag catcggtcgg cgatgcgttc ccgtcatctg cggaggctgt
    19381 ctgggcgatg ggcctcgagc tggagcgagc cctccgagca tcgatgtccg agctcgacca
    19441 ggcctcgtca gacgcggtca gcgcgacagc ccgtgatctt ccgggagcat tcggcgagat
    19501 tacgggtgcg gtcctaagac acctcggcgc cctgcccttg gatggcccgc gtcgcgcacc
    19561 gagcattgcg ccgctggagc tcgatgctga cgcatcactg ccggcctggg cgccgccgag
    19621 ccgaatcata ggggggttcc atctggtgcg gaccgtcggc actggcgccg tggggtcggt
    19681 gttcgttgcc cggcgagttg aagaaggaca ggacgacagc gcgcccgtgt tcgccctgaa
    19741 agtgcccgac tattccgcag ccgccgctcg tactctttct gaggacgaat ttcttcggct
    19801 cttccgcgag gaagccgggg cactcctggc gctgccggaa catcccaaca tcgcacgttt
    19861 cgtcacgttc gatgctggcg cccgacccaa gccgatcttg gtgatggagt tggtcgaagg
    19921 accgagcctg gagcgcatgc tcgagatggg cgacctttcg ctcggccgcg cgcgagagct
    19981 cctgctgggc gtaggcgccg gcctcgatgc gatgcatcaa gtcggggtcg cccacctgga
    20041 cgtcaaaccc tcgaatatca tcgtgcgcaa tgccaacgcg atcaccggcg tgggtcagcc
    20101 accccaaccg gtgctggtcg acttcggtct cgcaggtcgg catctgcggc ctggttgcgg
    20161 caccgcgaac tacggcgcgc ccgagatctg gggcgcaggc gatgtttctc aatcgatccc
    20221 ggccgacgtg tatgcattcg gttgcttgat cttcgaagcg tacaccggaa agacgttgtt
    20281 cgacgccggt ggcgaagccg cgatgatcac gcagcacgtc acgcacgacg gagaccctga
    20341 gccggttctc tccttgctcg aacgccccga gacccgtgag ctcggcgcac tggtcgctcg
    20401 ttgcattcgg cgcaatccaa gcgatcgcat caccatgcgc caggcccggc agtttctagc
    20461 caacttgagc cttggtgccg cggatcagca ttggccggtc cgcatcgcga gctagagcgc
    20521 cccccttaac gcgaagcggg ccccagacca aagcctggaa cccgccgcgc cgtctcagcg
    20581 cccaggggcg ctttcttgtt ccggggtgtt actcgctaac ctcggtctcg gtcgcggcat
    20641 cctcgggtcc cgtgtcgacg atgtggaact ccacgcgtcg gttctgcgcg agctcttcct
    20701 tcgtcttggc atccggcacc agcggcctcg taggtccaaa gccctccgaa atcaggcgcg
    20761 ctttgtccac tttgccgcgc ccaacgaggt agcgaacgac cgtgttcgcc cggttcgtgg
    20821 acagccgcat gttgtacttg aggttgcccg tcgaatcgct gtgtccttcg actcggatga
    20881 gagggatctc agggtgtgcg ttcatgacct gcgccacgtt gtcgagcagc gcgaaagagc
    20941 gcggctggag cttggcactt ccggtcttga aatagaccat ctcgagaatc tcgagctggt
    21001 ctgcgccgat cacgaccagt tgagctagct gacagccgtg gttctccacg gtgccaggct
    21061 cgtccgggca gttgtcgaca cggtcgggaa cgccgtcgcc atcgcgatcc ggatctggac
    21121 agccgttgtt ctccagcgtg ccaggctcgt tcggacactg gtcatcgaca tcgagaatcc
    21181 cgtcgccatc attgtccgga tcaggacatc cgtcctcgtc ctggaagcca tcgatgtctt
    21241 ctgcatcgtt cggacaccca tcgtcggcgt cgaggacgcc atcgccatcg ttgtcaggat
    21301 cgggacagcc atcctcgtcc tgaaagccgt cgaagtcctc cggctcgttc ggacactgat
    21361 cgacgtcgtc gagaatccca tcgccgtcag catcgaggat gggctcttcg cccggcatcg
    21421 tgtagccgat catgccaacg ccacgccagt ccggagcgcc gtaaccaggg gccacacctg
    21481 cgctgcctga aacaccgacc acgaaaccct tggggtgcaa gtacttgaag ccgccgagca
    21541 cctcgaccgg ggtctcttcg cgggtccaga acggcaccgt gctgtttgcc gcggttcgtc
    21601 cgaaggcctc agccgaaatc atgaacttgt cctgcgcgat catgaacaga agaccgcctc
    21661 cgaacgtgaa ttcgttgccg acgaagaggt cgggtgcgac cgtctgaccc tgcgtgccga
    21721 gcttgtaacc aacctggatc gggatacgga ccacgtcgcc cgcattgaag gtcatcaaga
    21781 gctcgaacca accgccgagg taaggcttct tgtcgacctg accggcgtac ttttgttggt
    21841 cgtcggagag cgacgaggtg ttgatcgtca tcgtcgcctg cagggcaagc tggaacacgt
    21901 catgcctatt gccgatgatg ttgccgcgtg cgccaaagta gacgtccccg aaatgcccgc
    21961 cgttcggaag aaggtagcca aacgggcccg acgctcctcc gggcagtgca tctcccgccc
    22021 cttgtccgga gtcgatgatg aagtgatagg ggatgtccat gtagatcacg aggtgatccc
    22081 agaggcccaa gttccaattg atgtggccgg tcagctgccg gtgcacgacc gaagatgtgt
    22141 tcccggtgcc ctgatcttgg aataccagcg catcatcgtt gtaatccatg tagatcatga
    22201 tgccgaagcg cttgtggccc tggccatccg ccgtgctgga cgcgaaacca tcggtcgcga
    22261 gctcggcagg cctgtactga ttcaggtcga tctgggttga tccctgctgg gcggagacgc
    22321 ccgatgcagc acccgacgca gccacgaaca tcgcaagcag cggtgcgacc caaccccggt
    22381 tcgactctct taggtcatcg ctcttcttca cgcggtcctc cattcagatc cttgggcgag
    22441 cggaagcccc ccgaaatccc cgatagtctc gcccctaggt accaccacgg taaaaaaaag
    22501 ggctgaaata agtgctccag aagccgctgc catagaaaga atccgctagg atcacatcca
    22561 tgcctccaag ggctcgatac cgcacggcgc cggcccccac gcgagggatg ccgagcggaa
    22621 tcccctacat cgtcgggaat gaagccgccg agcggtacag ctactacggc atgcgagcga
    22681 ttttggtcat tttcatgacc aagttcctga tgaacgcgca gggcgagctc gacctcatgt
    22741 cggacgatca ggccaggacc tggtttcacg ctttcgtcac cgccgtgtac ttcttcccca
    22801 tcttcggggc gatcatcgcc gacgcatttc tcggcaagta tcgaacgatc ttgctcctgt
    22861 cggtcgtcta ctgtctgggt catctgaccc ttgcagtcga cgacacgcga atcgggctcg
    22921 ccgtgggact cggcctcatt gcggtcggat ccggcgggat caagccgtgc gtctccgcgc
    22981 acgtaggaga ccagttcggg ccgacgaatg cgcgtctctt gcccaaggtg ttcggctggt
    23041 tctacttcgc gatcaacgtc ggcgcgctcg cttccattct gtttgcagag ccgcttctcc
    23101 accgcttcgg cccatcggtc gcgtttggtg tgcccggcgt cttgatggcg ctggcaacgc
    23161 tcgtgttttg gatgggaaga aacgaattcg tccacatacc gccgggggga gtcgcgttct
    23221 tgcgcgaggt gttcagtcgc ggcgggctca tggccctcgg caagctcttc atcgtctaca
    23281 ccttcatcgc catgttctgg gcgctcttcg atcagcaggg ctcgtcctgg gtccttcaag
    23341 cgcagcggat ggatcggcac ttcatcgtgg actgggaccc ttcacaggcg caatggctca
    23401 accccgcgct ggtgcttctc ttcattccgc tcttcaaccg ggtgatctat ccggcgtttg
    23461 atcgattctg gccgctcact ccgctgcgaa agatctcact cggcttgttc ctgacggtac
    23521 ccgcattttt gctaccggcc tggctggagg cgagactcga tgctggagaa tccgtcaaca
    23581 tcgtctggca gctcggtggc tacatcatca tgaccggcgc cgagattctc gtctcgatca
    23641 ccgcccttga gttctcgtac acgcaggcgc caagacacat gaaatcactg gtcatgggcg
    23701 cgtttctcat gagcgtctcg atcggcaatt tcttcaccgc ggcggtgaac tacttcatcc
    23761 agaatccgga tggcagcagc aaactggatg gagactcgta ctatttgttc ttcgccgctg
    23821 cgatgggcgt gactgccgtt cttttcgtgc cggttgcagt ctggttcaag gagcgaacct
    23881 acattcaaga agaggcgcca aacgaggcgg tgctatgagt atcatcgagc aagcgagaag
    23941 ctggatcgca agcgatccgg atccagaaac acgtcgggag ctagaggctc tgctcgccct
    24001 caacgacgtc gccgcactcg aagagcgctt cgctggacct cttcagtttg gaaccgccgg
    24061 acttcgcggc ctgctcggcg ccggtccgtc tcgaatgaat cgagtgacgg tggcgcgcgc
    24121 aacctcaggg tacgcatctt ggctgatgaa gcaggtgccc gatgccagct cgcgcggcat
    24181 cgtcgtgggt cgcgatgcac gacgcaatag cgatgcgttc gagcgggaag tcatcgaagt
    24241 tctggccgga tccggcgttc ccgtctgggt cttcgaggac gtcatcccga ctccggtcct
    24301 ggccttcgcc gtattgaagc tacgcgcagc aggcggggtg atgatcaccg cgagccacaa
    24361 cccaccgggg tacaacggct acaaggtgta ctgggagaac gcagctcaga tcataccccc
    24421 tcacgatcag gggatcgctg gggagattga gctcgccccc tcggcgctcg agatcccgcg
    24481 catttcgctc gaagacgcag aacgccgagg attgcttcgc aaggccgacg caatgcgcgg
    24541 ggaatacatc gaggagatgg cgtctgaggt aaggcgaggc caacgtgggc ccgagcttcg
    24601 aatcgcgtac acggcactcc acggggtcgc cgaaagcacc ttccgagcgc tgatggctcg
    24661 cgcgggcttc gacgacgttc aaagcgtgag cgagcagtcg gtgcctgatg gaacctttcc
    24721 gaccgtcgca tttccaaatc cagaggaacc gggagcgatg gaccgggttc tcgatttggc
    24781 gcagcgcatc gatgccgacc ttgcgatcgc aaacgaccct gatggtgacc gggttggagt
    24841 cgcggttcgc tgcgagacgg gctttgaaat tctgtccgga aacgagatcg gcattttgct
    24901 cgcggacgac ctgctctctg tccgaaagcg atccgggcag ccgctcgtga tctccaccat
    24961 cgtgagcagc ccgatgatgg gtccggtcgc ggcaagtcac ggagcccgtt gggagcaaac
    25021 gctgagcggt cacaagtgga tccaaaaccg tgcgctcgag ctgatggaac aaggtctaga
    25081 ctatgttttc ggctacgagg aggcgctcgg gtatgcgcca tccctggcgg tgcgcgacaa
    25141 agatggaatc agctcggctc tgagaatcgc cgacatggcg tcgcggctca aagcccgggg
    25201 cgaaaccttg ctggatgcga gagacgagct ctggggcaag cacgggttct tcgccgatcg
    25261 acagctctcc gtgaagttcg agggcgccgc ggctcaaggg cgcatgaatg cagtggtaaa
    25321 tcggtttcgg gattcgatcc cggaccgtat tggcggactc gcagtcctcc gaatgctgga
    25381 cctcgagcgg cagctgcagt ggacgccgct tggaatgagg ccgttcgccg atcttcctcg
    25441 gagcaacgtg ctggtattcg agctgcaggg cgggcatcgg ctgatgcttc gaccgagcgg
    25501 caccgaaccg aagctcaagt actattttta cgcagtggcg cctcatcgcg ccgacggcgg
    25561 actcagcgct tccaaggcaa gcgcgctcgc cgtgctcgat cgcatggtcg aatacgtcag
    25621 ggatgccagc gattgctgag caatgcgcgc gccaatgcgt cccaggcttg agaggcgcgg
    25681 tagagttttt ccgcgtcgcg cgtgaccccg cgatgccagg gcagaggagg acgcccgtaa
    25741 gagttcggcg ccggcaactc tgcgctgagc tcctcggcct gctgcgcgca cgcctgggat
    25801 tgacgaaggc agatatcgac gatgtcgtcg accggtgtca cgaggtgagg ggagatttcg
    25861 atgtcgacca cctcggcgaa atcgcgcgag ctgacgccgg cgagccaaag ggcgcgctcg
    25921 atgtcactgc cttgcgccgc cggcgatcgc ctggaccatg attgtggtcg caactcgcga
    25981 atgtccgaat gatatgcggg acgcccttcg acgggttcgg atgcttgagg ggccggctga
    26041 agccaggccg cgattcgcgc gcgaagcatg gactgcctca cttgtcgcgg accgcctgcg
    26101 ttgacgtcaa ataagctact ggagcgaggc tctgaggatg atcgcgaggg cgaccaagag
    26161 aacgacgcca gccgcgcccc agagaacaac ggagcttcgg cgtcgccggc tgtctctcga
    26221 aatagactcg ggcggttccg agaaccgaag gctttcggga agcaagtcct ggcgcgccac
    26281 gaggtcttcg agacgtgtcg ccgtcgtgat cggcatggcg agcggatcga atcgcggttc
    26341 gcgactcgcg gcgtctgagg gcgcgtttac cgacgggcgc ttgctgctcg gacgcacgcc
    26401 caccgatgcg atctccagca tggggccacc gcggccgagc tcgatctcgt cgccgtcgtt
    26461 gagggcagca tgcttgatcc gctgtccatt gagccaggtg ccgtttcggc tgcccgtatc
    26521 gatgagaagg taacggccat cttcattgcg gatctcggcg tgacgtccgg aggcgtccaa
    26581 gtcgacttcc ggatcgaagt tcacatcgct atccggcatc cggccaatgc gaatgagatc
    26641 ttgttcgaac tcgaattcac gtccggcgtc ggcgcccttg atttggcgaa gtcggatcat
    26701 gatcgcgcct caatccgttg gcggaatgag aaccacttcc ctcggcgacc cgggttccac
    26761 caagacgtcc tttccaacgt gagtcccatc gacctcgcga gcctcgaagt aatatgcgcc
    26821 aggagcgagc cgaagtagga ttgcctcttc gaggcgcaca gggccatagc ttcggccgtt
    26881 gacgaaaagc tcaccgcttt cgagtgccgc acggacttcg acctcgaccg gaatctgaga
    26941 gtcggtgtac gcaggtagtg gtggctgcgt cagggcaccc gtagaaacgc cgcgtcgacc
    27001 cgcgctcacg accgtgatca ccgctgccaa gccgagtaag gcgagtaccg atgcggcggt
    27061 cgaccaggag aaagaaccat gcgtgcctgg ctcggatggt actgtcgctg gatccgggcg
    27121 ctgcggcgac atcgcgcggg gtaccatact tctaaccaca tgcggcaagc tcggccctcc
    27181 ggcggggcgg ccgtccttgc cgcgtggcaa cgggtattgt tggtagaacg gaatcgctcc
    27241 ggcttcgggc ggctcgagcg catcgccatc gaagtcggca acgatgcagg tcacgttgtc
    27301 atgacctcct gcggcatttg cgagctcgat gagccggtcg gcgagctgcg gaagtggccc
    27361 gatgcaggcc atctcctcct caatcgtggt gtcgtggacc agtccgctga ggccatcgga
    27421 gcacagaagc aaccggtctc cacggcggag ctccacgaaa gtgagatcca cggcaacctg
    27481 ctcggtcgta cccagggcct gtaggatgat gttggagtgc tcgaacgcct cggcttcttc
    27541 ttcggtgagc tggccggctt cgatcagttg attgacgagc gactgatcct tcgtgatcag
    27601 gctcaagcgg ccgttcctga ggatgtaaca acgggagtct ccgacctgcc ccatgaacag
    27661 cactttgtcg acgagtccag ctacggtcgc agtggttccc ataccccgtc gagttcgatc
    27721 cttcttggca gcgaggaaga tccgagcacc ggcatcttcg atcgagtcga cgagacgcct
    27781 ggcgaaactg tcgcgatcct tcggcggctc attgcccctc aatttctcga agagcgtgtc
    27841 caccgccatg tgacttgcaa cctcgccagc ggcagcgcct cccatgccgt cgcagacggc
    27901 catcgccatg cccctgtctg atacggttcc cgacacctct tgctcacctg caaacggacc
    27961 ggtctcggcg ctgagatcgc caaccatgaa gttgtcttcg ttgtgctccc ggatgagccc
    28021 tacgtcggtc ttgccgaaaa tcgtgaagtg gagaccttcg ggcaccggct ccgaggctgc
    28081 tccgcggccc ggactcccgc tgtccgcact ggttgtggga gccagcgttt cctcaccctc
    28141 actcggactc gaccgatcat caccatttcg gtgcgcgtcg ccgctcattg agcccccgcc
    28201 tctatccact gaccccacca agacaccata acgatacagc ctatcgccat gctgtcgaaa
    28261 tctccaccgc ttccggggct cccacccctt ggatttaggg gcgattagtg gaacgaagag
    28321 cgagcccgca gggagatcga cccgggcaga gctctccgga gcactgggct cgcctactct
    28381 attcgcactc ggagtgcaga tagcggacag acaagattga cagcacccca ccctctctta
    28441 tagtagcgcc gattcgactc gctttgaggt gttctgatgg agattttcct gaataagcgc
    28501 gcgatgcggg cgctcaggct ttcggttgcc gatgcgatcg aggatggtga aattgatgcg
    28561 ctacgagatg atctggtcag cgccttttcc gatgatgaca tcgaggaaat cgaacgccgt
    28621 atcgacagcg gggatttcta cgacttcgtt tcggagatcc tggacgagtg gtccggagag
    28681 gatttcgacg agctcatcga gctgatcgag acacagctca gcgatgcgga cgtggatctg
    28741 aaatataccg cgaccgaggt cgaagaggac gacaccgaag aggccagcga ggaagaagac
    28801 gaagacgaca ccgatgacga gctcatcgtg gacgacgagg atgaagaact ctgaggaaaa
    28861 cggcacagtc gccgctttcg gagtgcctcg aaaccccgcc taattgacag gccccccccg
    28921 tcacgctacc agcatcgccg cgctcgttga gagcgatccc taggccaccg tagctcagtc
    28981 ggtagagcaa cggtttcgta aaccgtaggt catcagttcg attctgatcg gtggctccat
    29041 cttttcgggc acttgcgtgt atggtgaatt tggcgttgac cagtcgttga ccaatccggg
    29101 agccactttc gagtcgtcgg accactgcgc gtcatatcgc ttggtgtgca tcgctccgtc
    29161 tcgggcgtga aagacgaggc ctgccgccct cgtgacgtcg tccctgcgcc tccagagcgt
    29221 gagtgcctcc aggacaggcg gcaggtcaca gaagttgcgc gagatcgcga ttttttttcg
    29281 tgtccggttc cccgaagggg atcgatccgt ctgtgactct cagcgacaaa attggtcaac
    29341 atgggaccca actcgttggg gcacgggata gttttgtctg ccaacaaaac tgctcgcgct
    29401 gccttctccc gctcgtcgcg tgatcggtcg cgggagttgc gcgctcaacg cctccggctt
    29461 attcgcccac gctgcgcggt gcagcaggta gtctccacca acgccctttc agcttcgcgc
    29521 cccacgcgac acaacgagct agtaccgcgc aattgggcgt ttacgatgag cccgatgggc
    29581 taagagagct aagagagccg atccgcgcta cacggcgtaa gagtgtcgtc cgcagactcg
    29641 acaccgaaga acgaggaggg ttttacatgt ccaacgaagg ctaccatgaa ccaatcgctg
    29701 agttgtcaga cgagacccga gacatgcatc gcgcgatcac gtctttgatg gaagagctag
    29761 aggccgtcga ttggtacaat caacgtgttg acgcctgcaa ggatgccgag ctcaaggcca
    29821 ttctcgccca caatcgggac gaagagaagg agcacgcggc catggtgctc gaatggatcc
    29881 ggcgccgtga cacgaagttc gagcatgagc tcaaggacta tctgttcacc gacaagacaa
    29941 tcgcccacga gtagtgtgaa caaccccccc tctagtctca gcggctgccc acgtgttcgt
    30001 gcgctgccat cgggcgcaac cggtcaattt ccgtgccttg cctcgagcgc gccatcgacg
    30061 atgaagctcg gggcaccgaa gatgccgagg cgccgcgcct catcggtctg tgcgcggagt
    30121 cgttctttca tgggtggagc cgtcctgtgt cctctttcgc acacagcgaa tctcgggtgt
    30181 gccatgaccc cacgtcagaa acgcttcgcc ttggtgctcc caaaacgatt cgtttccgcc
    30241 tgcgtacttg gcgccacttg cgcttggttg aagaaacatc aacgagaccc ggtcccctcg
    30301 ctccacgacg accgtgggcg gatccgttgc gaagagggtg gcgaccagct cgctgcgtga
    30361 gtcgtcctcg cacgtgtaag acatcggtcc agtcgcgtga acgaggtggt atcgagcctg
    30421 gagcgctgcg attcgaagct ggtagctgcc ttcgacgcag gcgcgcgggg cgctgctctt
    30481 ccaacaatcg ttgcgcccct tgatccaccc gcgctgttct gcccggagca cgtgcgggcg
    30541 ttcgtccacc gccttccgtg acgccgcggc atagacggat gccatcgttc gatcgaccgc
    30601 agacagcgcc tcgtcctcgc agatcagctg ctcgatgctg cctcgggcct tgctgcagtc
    30661 gaacgacggg cctgccgccg tcgccgcggc tgacggggct agccatgtag acaccgccat
    30721 ccagacgagc gttccggcga ccgtaacaat cggaaatgat ttcatcgaga gctcaacgtc
    30781 caaagcaccc agatccttcc cgcgcgtcac cctgcgggta cgtccggatc cgacatcatc
    30841 tccgcaagcg atggaaacct gagggaaatg acatcgccgt catgcacctt tcgcaacccc
    30901 tcgacgagga tcttgtcggt ctcggtgagg ccctccgcga cgacatagag gtgagggatc
    30961 tcctcggcaa ccgagagcat agcgaattca aaggccctgt ccgcgctgcg accaacgcga
    31021 cgtaacatcg agtgccgcgc aattgggcgt ttacgatgag cccgatgggc taagagagct
    31081 cccgaaaacg tgtcggcaca aacaggcctg gcgacggcga ccgctccccg ctgagagggc
    31141 gggcttgttc agccacgcta gctttgcgcg acacgtttcg gcgcgatgca ttccccgtcg
    31201 ggaagcctac tgactctccg cgtaccgggg taagtccagg gcacgcgctt aacagtccac
    31261 cgaggcggat caactccaat cttgaatttg ggtcttcata aacacacgcc gatgcacgtt
    31321 ccggagatgc actcggcgtt ggcggtgcac ggattgccag ggccgagatc gttgatgcaa
    31381 tcgccaaaaa ggttgcagtg cttgtttgcc gcgcattggc tgtcgcggac gcactccgcg
    31441 caaaagcccg ccgaacagat gttagacaga cattcggcgt tggccgtgca cacagcgttg
    31501 ttgctgaggt cgttgatgca gtccccgaag aggttgcagt gtttgttcgc cgcgcattgg
    31561 tcgtcgcgaa cgcactccgc gcaaaagccc gccgaacaga tgttggacag gcattcggca
    31621 ttggccgtac acacagcgtt gttgccgaga tcgtcgatgc aatcgcccaa gaggttgcag
    31681 tgtttgttcg ccgcgcattg gtcgtcgcgg ccgcattcac ggcatacgcc cagcgtgcag
    31741 acaccgctcg tacacgcgtt gctcgtactg caaccactcc cctcctcgag aggcctcgcc
    31801 aaacacacgc cccagttgca gatccccgac gagcaatctc cgttcgcgag acatgaagga
    31861 ggtgtgcatt ctccgtgaac gccacagttc tccgattcgc attcggtatt caaaacgcaa
    31921 ctcgcaccgt ttggtaagtc acccttgcag tcgcgggagt tggcgacgga ctcgccggcc
    31981 atttcatcgc atgttccgtc ggtcgtgaag tcggggcaac actgacagtt gcgtccctgg
    32041 ccaccaaccc agcccgtttc gggttggtcg gcgaccgcct ttgcgtagat gagcgccaac
    32101 acatcgcacg tgatgcggca cggacccacc gcataagtgg ccgcgcaggt atctagcaag
    32161 cgcgatcgaa actcgtcgtc gcactgggcg cgagtcgaac cgcagaccgc atagcagtag
    32221 tcgtgaagct cgcatgcgct cctgaggtcg gggccggccg tgttcgcgtt ggcgttctcg
    32281 ttgccaaagg gcaccgtgca gaggccctcg ctcacacgct ccaacgggtt gtccgcgatc
    32341 ccctgccgca cctccggatc gtcggggata tcatagcctg catcttcgag cgcagcgatg
    32401 aactgttgtg cgctgcaccc atcggagtct ctcgttggcg attctccatt ggggccacgc
    32461 gcatcaaaac actctcccct ctggccatcc ggcgcaactt cgccaacgca aggcgcacat
    32521 ccagtgtcgg tgcactgagg cctctccggt gggcactcgc tgtcctccag gcagagcctc
    32581 tcgcggcaac tcccatcgat gcacgcctgc atctcacccg cgcagtttgc tttggaaagg
    32641 cactcaacgc agctgccctc ggcaaggttg cagagaaggc catctgtttc gcacagtgag
    32701 tcagggctgc acgggagcgc gatccggcca cccatgccac cggtgccgtc cgtgcctgca
    32761 gtcccggccg cgccgccggc tcctcccgct ccgccgcgag cacccgcgcc gtcaactccc
    32821 ccggcgccgg ccgccccctc gtcgctgccg catccgccga gggaaagcgt gaccagggcg
    32881 aatatccaga gtcttccaac ttgcacccgc atcgattccc tttccgatca ggcgacagtc
    32941 ttcctcgccg agcacccgga cagatgacct tacatgtttt gcgagtgagg cggaagagaa
    33001 ggttcctccg gcgggagatg aacgaagatc cgaaaacgcg ccgacgtcgt acatcgcgtc
    33061 ccgcggtcac tgcgaagtac agatgcacct aacgcttctg cactggtagc ttccgataag
    33121 gcaatagcaa cctcaccctg gttggcgtac gccaccgcgt agatcacgag aggaacggcg
    33181 ctggaaaggc acggagccgg caaccacggt cgggtcgtct aggcggcctc taaggaaccg
    33241 gcgcccgtac ctcgcgaccg atgacggctc cgatggcgaa taccggaatg acattccagg
    33301 aaaggaagtg ggacaatcga aggttgcctt tggtgtgttt caggatctcg tatcgaagcg
    33361 tccagcccat gaaaaaacga ttgatgtgaa ggtcgatgtc gagcccgaac cgagctccga
    33421 cgacggctga ctgctcgctc tggttgaagg aaccggttcg atcggtatac gacttgccag
    33481 cggccacgct tgcgtgcagc gtcagggtcc acaagagtcg tcctcgctca cgcgctgtcc
    33541 ggatgacgta ccgatagccg tatcccgcat gcgcaacgac gaactccgtc cacaactgag
    33601 gtggacagtc gaagatgcac agaaattcgg cgtcgctgtc gacgtctatg tccaatcgat
    33661 agtcaacgtc gaccaacacg ccgtgaccgt tcttcggata gaagcgcaca cccaaggccg
    33721 gcaccacata gaggctccgc ttccccgagt cgggtaggtc catggcgcca gagcgatagc
    33781 tcgaccagcg atacgacgcc gtgaagaggc ggaatctcag ctcggtcgaa agcgaggcgc
    33841 ccttctttga ttcgctcgtc tgctcgacgt cttgcgccgg aactcgttga aacgatccgc
    33901 tcagaagagc tgcgaccagt gccgccaacc cgagccatga agaacgcatg agcagcctcc
    33961 caggtcggta cccgccgcta gtgagatgca tcagccgtgc cagacagaag ccccgtttct
    34021 ccaggcgatt ccggtacagc ggcgcgcaga tctttcgtat gcggcatgga ggcgcagcag
    34081 gtgcgcggtc ggcatcggcg cgtgcctcgc gacaatctgg cgcacactcg attctgttca
    34141 tcgagcgcac aatcgaccag ctaacccagt cgaaggacat cgccgcgctt cgaaaagtgg
    34201 ggggtgatac cggatccgct aggggaagtt cgacgcaaac cacttccaga tgcccacggt
    34261 cgacaagctc acgtcgtgtt ttcggtaaat ctcgccaacg atcgatcggt gtcgctcgtc
    34321 gaagtgctct gtcttcggag caatcgccag atcaacggac gtgagttgcc tgcgaattcc
    34381 tggtgattga tacaaagatc ggtaggccgg ttctggtctc ttcgctggcc gtcacgccgg
    34441 gccggcggtg ctactggccg aaagtcgctc ttcagccatt cgcaagagct tcacgcggtc
    34501 gagcttgcca gtggcgttca gcggcatttc ggacagaaat gcgatttcct ccggcgcttt
    34561 gtagccaaca cgatcgcgag cgaagctgat cagttccgag gcggagggcg gcggcgctcc
    34621 ttccttcagt gtcacgtagg ctcgaacgtt ctctccatgc acgagatcgt gaatgccgat
    34681 gaccccggct tctgcgatcg atggatgctc ggtcagggct tcctccacct cttgagggca
    34741 gatgttcgag ccatcgtgga cgatgatctg cttcttgcgg ccacaaaacc acaagtatcc
    34801 atcctcgtcg accctcatca tgtcgccggt gtcgagccag ccgtcgacga ttgtttccgc
    34861 agtggctttc gggttttccc agtaaccgat catattggct tgggagcgaa tccagagtcg
    34921 cccaggcgtg ccgaccggta cttcggcacc cgaatcatca cggagcgaca tttcgtatcc
    34981 gggaatgagt ttgccgatcg agcccattcg gttttcgccg tagggcggat tgatcgtgga
    35041 gacgccgatc tccgtcatgc cgtagctttc atcaatcgga aatccggcag ccgccgtgaa
    35101 ctcgcgttcg agctccgcgg agaccttgtc gccaccggcc gaacacaagc gaatcgaagc
    35161 gaagtcactc gagcctgcgt catggtcccg gaccagggca atcaacgccg ccggcaacat
    35221 ccacagcacg gtggggcgaa actctcgcaa cagcggtaaa atctcaggac catcgctgcc
    35281 gcgtgaaatc agcaggcgtc cccccgcggt gagcattgat aacccgagca acgaaccggc
    35341 gatatgcgag agcgacgagc cgggcaggag cgtgtcggtg tccgtgaact gaacgccgtt
    35401 gatcgaagcg tcgatcatct gaaaaaacgt cgcccgtgtg tgcgtcaccc ctttgggctt
    35461 gcccgtgctg ccggacgtaa aatagatgag cgttgctgcg tcagggtcgg gcgttggaaa
    35521 ggttgcagtg ggttgcggcg cttcaaccaa ctcttcgtag tgacggccgg caccgtcatc
    35581 cgctttgtag cgaacgacgc ctttggatag tttgtttccg agcttgctgg cggcgacatc
    35641 ctcgtcacgt tcggcatgat gtaacagcaa tgacgcaccg ctgacttcca aagcgtgatc
    35701 gatttccggt gccatgtacc gatagttcag gggcgtcgcc accaaacctg ctttcagaca
    35761 ggccaggtaa tgaatgatca aggccggccg attcggcatc agcgaagcga cacgatcgcc
    35821 aggctgcaaa ccgaggtgca ggtattggac cgccaggcgg tcgctgatct cagcgagctg
    35881 acgccagctc atctgcgtct tgaccgaagc caatgcaagt tcgtttggtt ttgacttgag
    35941 accgcgctcc aatggatcgg aggctggatc gatctcgact ctgtttgctc ggttaagagg
    36001 catcatgttc aacggaaagg atgaggatgg tgccagaatg gcacaaccga tgtttgcgta
    36061 tcgtagcaga aatcgtgagt agcctaggct ccacctgccg atccgctgaa gatcgaaatg
    36121 cgaaaaatgg cctaccatgc gcttcctcgg atcacgcgca tggttccccg gtcacctctc
    36181 ggtcgaacac tcaggacaca tcgcgcaccg acgtcacgca cctacggcga tccacgcttg
    36241 cccagaatgt cgagatgtaa cgctcgatga gcccgcgcgt ctcggggtcg cgctcggact
    36301 cggcggctgt caattgatct tgagcgcaat gctgacgacg atctgatagt cgttctcctt
    36361 gggcacggtc ccgtccgctc gtggctgtgg atcccgggtt cgcaggaata agaacgtggt
    36421 gaggaagctg aacctgtccg tgatttcgac gctaacgtcg accgtcccgg tgtggttcgt
    36481 gctcccgaaa ttcgtgtagg cgaggttggt gcgccagtcg atcgtgagct cgacgttgtc
    36541 gttcaagaac tccagcttcc aatgggtccg gagcaagatg aatccatcgc tttgcggatt
    36601 cgtcgcacca gttggggtgg agaggaaccc aaggaactga tacccgaggc cgctctgcaa
    36661 gtcccactcg aactggttgg tatgtttttt tctctgcctg gtcttcttga agagatagac
    36721 cccggcccct gtgctgggcg tagctcgcag cttgatgttc tggaacgcat cgtagagaag
    36781 ctgcccggcg atcggaatca ggtagacacg gtcccagagg aacagagcgg tattgacgtc
    36841 gccgaggtgc cgattgacgt tgagctcgcc gtcggtgtac gccacggttc cattgtagcg
    36901 gagccctccg agcgtgtggc catccatgcg catcaggctc cattgcgtgg tgagcgaacc
    36961 ctggctcgta tttcccgcat tggcggagaa cccgaggctg agcatcgtcg accaccatgt
    37021 ccgctcccga ggcggtccct cggagatcga gaggagctca gcgcggggat atgtcttgat
    37081 gccttggtcg ttctcgatga tgaccgcgtc tttggtgatc atcgctttgc ccgagacttt
    37141 gaccttgtcc ttgaaggtgt aactcttgat cttgggagaa tggacgccgg cgatacttcc
    37201 ccaactcagc gtcagactgt cgagcttgtt gctgtagaag ttcaagttct ggccggcttc
    37261 gagccctttt ggacgcaacc aacggagatc gccccttagc cactccccgg tcttgagctc
    37321 gatccagtcg tcggtgtcgc cgaactcgag cctgctctcg gtagtgagac cctcggcggc
    37381 ctctcgctgg gcctgcttca gggcgctctg aacactcgtc gcgtctccgc ccgtctgggc
    37441 atgcaccgcg cgggggacgc agcacgcgcc gagagccacg accgcgagcg ccatgggtcc
    37501 gactctgcgc tttgctatgg gtcccgaatc ctgcacccag ccgcggtata tacgctgtgc
    37561 gttcctgccg gcaaaaagcc tgcgacgatg cgaattgaag cattccgtgc ttttcacgga
    37621 cgcagtcgcc gaggagctcg attggcgtgc ttaggattgg gtcatggtga aactcctagg
    37681 ggggctcact cagcgtcgcg attgcacaga acacctgatg gggtagcttt ccgagagcaa
    37741 atggcgtgtt tgaatcgatc atgctcagcc ccgacgtcgc ccttgattga gagacatcgt
    37801 gccggacact gaccgggact ccgacgctgc cggcttcttg atctccaatc cgtcgaaccg
    37861 acgcaagagg aagcgccttt atctttgggg cgctcttgcg gtgatcgtcg tcaccggcgt
    37921 cggcatctgg gcggcacccg ttcgtcccga tcgaaggcag atcagaacgg aagaggcacg
    37981 gcgcgggaac ctggtcgtca cggtcacggc gacagggaat ctcgagccta ccaaccaggt
    38041 cgaggtcgga gtcgaggtat ccggcacgat cgcaaccgtg gaggtggatt acaacgatcg
    38101 tgtggcgcgc gatcaggttc tggcgaggct cgaccccacg aaattcaaag cccaggtggt
    38161 gaaatcacga gccgctcttc agtcggccag ggcgaggatt ttgcaggcgc aggccacggt
    38221 ggacgaaagc cgcgcgcagc tcggcaggct cacccggctc agggtgacca gcaaagggac
    38281 cgatccttca caactcgaca tcgatgcagc ggacgccgca ctcaaacgtg ctctcgccga
    38341 tcgggcaagc gcgaaagcgg acgttgccga agctaacgca aacctagagt cgagcgaaat
    38401 cgacctcgcc aagaccgtca ttcattcgcc cgtcgacgga atcgtcctag tgcgttccgt
    38461 ggagcccgga cagacggtgg ccgcttctct tcagacaccg attctgttcg tgctggccga
    38521 ggacctgacc aagatggagc ttcatgtcag cgtggacgaa gctgacgtgg gccgagtccg
    38581 acccgggcag agggcgacgt tcatggtcga cgcctatcca gaccgcacct tctccgcaga
    38641 gatcgccgac gttcacttcg gggcacagga agtcgagggg gtcatcacct acgaggcggt
    38701 actaaacgtc gacaatggag acttgtcgct acggccgggc atgaccgcga ccgcgacaat
    38761 cgtggtggaa gagctgagag atgagcttct gatccccaat tcggcgctac gcttcagtcc
    38821 ctcgctccgg aaagaagccg agcccgatgc tggtcttctc ggtcgactgt ttccccgtcc
    38881 gtcccgctcc cctccgaaga accggcgaga gcaaggctct gcaaagaagg aaccacgggt
    38941 ttggatcgta cgagatggcg agccggtcgc aataccgatc accacgggag ccaccgacgg
    39001 aatcatgacc gagttggtgc aaggcgacgt ggagcccggg atgccgctca tcgtcgagat
    39061 gactttggaa tgacttcatc gtgcctgcgc aggaacatac tcagacaccg ctcattcggc
    39121 tcgagggcgt cactagagtg tacggaaccg gcgatgcagc cacgtacgcg ttgcgcggca
    39181 tcgacgtcca gatcggtcgg ggggagttcg ttgccgtcat ggggtcgagc ggctcaggaa
    39241 agtccacgtg cctcaacatt ctgggctgtc tcgatactcc tactgcgggg tcgtatcttt
    39301 ttcgcgggat cgaagtcggc aacctgggtc gcaaccagcg tgcgctgttg cgccgtcgct
    39361 attttggttt cgtgtttcaa ggctacaacc tcttgagccg gacttctgcg ctcgagaacg
    39421 tcgagcttcc gctggtctac cgcggcattg cccgggccga gcgtcggagg ctggcgtccc
    39481 aggcgctcgc cgcagtcggc ctccaagggc gggaaaccca cagcccctct gagctctcgg
    39541 gaggacaaca gcaacgtgtc gcgatcgcgc gagcgctggt caccgaaccc gacgtcctgc
    39601 ttgccgatga gcccacggga aacctcgata cggctcgaag cctggagatc atggatctca
    39661 tgactgcact caaccgggag cgaggcatta ccatcatcat cgtcacccat gaaagcgcga
    39721 tggccgccta cgccgaccgg atcatcaatt tcgtcgatgg cgaggtggag tcggtggtgc
    39781 accggggcga ggtagcctga tggtgtacaa tgcgtttcgg ctcgcgctga acgagattcg
    39841 gcgcaacgtg atgcgttcgt cgctgaccat cttgggtgtc gtgatcggcg tcgcggcggt
    39901 catcaccatg gtgacgatcg gcggaggcgc cacggttcag gtgaccgaac aaatcgcgag
    39961 cctcggcacc aaccttctga tcgtcacgcc cggccaacgg atggggatgg ggcagcgagc
    40021 cgcggctccg cctttccaga tggacgacgt ggaggcgatc ggcagagaga ttggcacggt
    40081 cgccgccatc gcccccatct cgtcccaacc catgacggcg gtgttcagca acgcaaactg
    40141 gagcacgacg gtcatgggaa cggacaatca atacttcaag gttgggaact gggccctcga
    40201 aaccggccgg gagttcaccg agagcgagct tcgcgccggt agcgcagtct gcatcttggg
    40261 ggccaccgtc cggcgggagc tctttggcaa tcaggacccg tcggggagca agatacgtct
    40321 cgggaagctc tcctgtgtgg tgatagggac gctcctcagc aagggtcagt cgacgatcgg
    40381 ccgggatcgc gacgacgccg tgctcgttcc gctgcgaact tttcaaagga gactcggcgg
    40441 aaatcaagat gtcggtctca ttcaagtgtc cgtgcagaac ggggcctcga cggaaagcgc
    40501 ggaggaggac atcagcgagc tgatgcgcaa gcgccgtcac atctcggcat cggaggacga
    40561 cgacttcacc gtcatggacc tgaaggaaat caccaagatg ctcgcgggga cgacgcaagt
    40621 gctcaccgcg ctcttgggag ccgtggcggc cgtgagtctg ctcgtcggag ggatcgggat
    40681 catgaacatc atgctcgtgt ccgtcacgga gcggacccgg gaaatcggca tccgtcttgc
    40741 ggttggggcc ctcgaacgcg aggtgctgac gcagttcttg gtcgaggctg tcgtgctttc
    40801 atcttttggc ggcggggttg gggtaatctt ggcgctcgtg gtgtcgatcc tggccgcgaa
    40861 agctttgagc gtgcctttcg tattcaacct ggggatcgtg atcgtcggtt tccttttttc
    40921 cgtcggagtc ggcatcgtgt ttggatactt ccccgctcga aaggccgcac gcatggaccc
    40981 catcgaggcg ctccgccacg agtgaaaccg gcagcgtgtg tggagcacgg acacgcagcc
    41041 ctccagatgg gttcgtgtcc ccgtgagcaa cggaggttgg aggaatgact tggctgatag
    41101 caacttcaaa ggccctcacc aggagcccga tgggctaaga gagcgacctg tcgcacgtag
    41161 tcaggccagg tgtcgttgtc gagcggggtg acggcggggt ccatggcgcc tgcggagcgt
    41221 gtacgatttt tcgcctgcct gagaagaacg aagagcagca acaagaggat gacgatctga
    41281 tccatgggat gtcgcgcgga cagcagctac gcgagcgcca acctcggtca ggtcttttag
    41341 atcttgtttt ttgctactcc gaaaactctg ggaacgtggt aacgctcgct agatgacgag
    41401 aatcatatcc tggacactcg ggtttctcgc agtcgcgttg atagtcttgg tcgccgccgt
    41461 cgagctcaag aatcggagcg gggttcggaa ggcgaccacg gaaatcactg cctaccaacc
    41521 gccgccgctg tctgactttg gcgccacgcg caccttgacc atcctgccac tcatcgactg
    41581 gcacgcgtct cgctcaggtc tgcgtaccga gatgggcgtg tcgtacctca tagaaaccga
    41641 cgagcatcgg gtcctgttcg acgtcggaca gaacaccgac catgagttcc cctccccgct
    41701 cgagcgcaac atgaaagagc tcggtatcga gttggagagc gtcgaccgga tgttcatctc
    41761 acacaaccac ttcgatcacg tgggcggcaa ggccaatcag aagaaccaga ccttctcggt
    41821 tgggctcgag caggtgccac tgcccgacgt actggctttc gtacccatcc cgatggccta
    41881 ccccgggctg cgccccattc acgcgcgcga gccgacgaag gttggggtgg ggctcgcttc
    41941 tacggggacc atcccgcgtc agctcgtctt cggctggatc gaagaacagg cgcttgtcgt
    42001 gcaagtcgag gggcgagggg gcgtgctcat cgtcggctgc ggccatcaaa ccattccgag
    42061 gctgttggaa cggtacgacg aagttttcag cgagccgctg tatggggtcg ttggaggcct
    42121 tcactacccg gttcccgagg gacggctgaa catgatgggg gtcaacgtcc agcggcggct
    42181 cgcgtccggt gacggaatgc tcgcacccct cacaatgacc gacgtggaag ccgagctgga
    42241 cgcgctgcgc gagcgacatc tcggtgtgat cggggtcggc ggtcacgaca gcagcgacga
    42301 ggtcatcacg atgttcgcgg acgctttcgg agacgcgtat cgacatgttc gggtgggcga
    42361 gcccatcgtc atcgcgtccg aaaagtgagt ggcggaaaga catccgaccg acgttgccct
    42421 aaaggcggca gggaccgggc gattccgttg tgttgtcgtg gagggagaca tctgcgggcg
    42481 aggtcgccgt gagctgggat gtcgttgctc ggcacgtaaa gcaaggccgc gctagactcg
    42541 ttcccgtgct agggttccgc ccttatgaat agtcagcttt tgaaatcgat caccctcgcg
    42601 ctcagcctcg cttgcctggc aagcatgggc tgctcgtcgg atgcctcgtc gaccgatcag
    42661 caggttcccg aagaggtcac cagctgggag cccgagggag cgagaaccgt cggagagtac
    42721 gcatacgata cggttcccgt tcccaacgcg ttccacacga tgcatgcggg acccaacaac
    42781 acggacaacg tgtgggtcgc tacggcgccg caacttgagc tcgattgggt ttcggagacc
    42841 tcattctacg tgcccgaagg accgacgttc gacaacgagg ggaatctcta cttcagcccc
    42901 ttgtttccgc agatcgatgt ttcgctggtc tcgctcgatg ccaagaccgg tgagcgcaac
    42961 tgggtggtac ccggggacgg aagcaacgcg ggctcgggcg ccgtcctgat tctgaacgac
    43021 cctgccaatc ccggacagca gatcgtgtac cacgcgacct acaccgaagc gatggcgatc
    43081 aagccggacg gcacaatcct atggcagacc ccaacgggtc tgagcttgcc tcccgtggtc
    43141 caaggcgagc gaagtcccac gcattcgttt ggcttcaatt atcatccgcg aaccgattcg
    43201 ctcatcggtg tcaccctggg cggagacgta ttcgcgttca accgagtcac gggcgagagc
    43261 gtcgcgccag tgatacaaat tcctggcgca ccagcgctca gccaagagct cgcgctgcct
    43321 cagttcgtcg ttgatgcgag caacgatttg accgacgccg tgtttggtac caccccgagc
    43381 ggactgagct tttactccgt aatcgtcgac gtgatcttcg ggggcggctc caaagttacc
    43441 aactacttcg cgatcgaccc gaatacgagt gtcgtctacg tcgcagcgac cgccccagac
    43501 gaggacgacg ggaccgtcga tggggtctcc gagctcgggg cgatctatgc cctggacttg
    43561 gtcgatgacg gaagcggggg attggagctc cgcgtgctcc actccgccag cttcgaaggc
    43621 ggaaccggct cgacaccgtc cgtgagcgaa gacggacgac gggtatatgt gtcggacaac
    43681 atcgggaacg tcatcgcata cgacagtgag ttgaacgaga tctggcgctt cgatctcggc
    43741 gctcctgttg cggcttcaat tgcggtatcc ccagacaatg cggagctcta cgccgtcact
    43801 caggaagatg tgttcaagct catcgacgaa ggcgaaaatg cccgcctcga ttggatggca
    43861 accctggaag ccttcgagga cgacccggag atcgaagtcg tgttccaggc cctgacgccg
    43921 agcatcacgg caaacggcat cgccgtgagc gtaggaggcg gaaagctgct cgcgggccgc
    43981 ggaatcatgc tgcgggttgg ggtcgggctc ttggatcgcg taacgggcga gctgcgctcg
    44041 ttcacggagg gccgtgagga atcgatcgcc gtcacgagca tcggccccga tggcggcatc
    44101 tacaccgcga gctcaccggt tcggcgagtt tccggcaaag cgctctaccc ggatctcacc
    44161 aaggacatca tcggcggcat cagccgctac aaacccgtgc gaaacgacct gctcgccaag
    44221 gatgcaagct gcgcggcagg cacgcgcgcg cgtaacgccg cgacgattgc ggatgcggct
    44281 ccccgctccg cagcggagga catgcgtcag atccaggtct tgatcgacca aatccgtgcg
    44341 gccatcactc gcgcggtcgc agacggtgac ctcgatcaag ccactgccga cgctctcgaa
    44401 tccgacctcg atggcgcgga ggctcatctc accatggacg ggctctcgac cgtcgcttcg
    44461 gatcttcttc gcgtgtgtaa cgcgatttga gcccgagccg atgcggcccg gctgactacc
    44521 tggcaaagcg gatgattcag cccttcagcc aggtataggc ctcgcgcagg gtttcgttgc
    44581 ccccactctc gaggatgtcc ccgtgcgcca gaacgatccg gtcgaagtcc caactgagaa
    44641 ttcggtcgag tgcgcggcgc gctcgcttct tatcgcggaa gatcattcgg agtgggcgac
    44701 tgagtccggg cttgccgtga atcccgccaa gcttgagata cgcgcgtgtc ggccaatgag
    44761 gtgaggtctc gaaattctcg atgaggtctg cgacgatcaa tgatcgcgat gggcgatgaa
    44821 gaaacaccat ttcgttcagc atggttcctt ccagcagcgc ggtgtcgagc tgctctcgcc
    44881 aatcgggaaa cgtttcaccg gcgagctccc ggtcgatcac gaggtccttg cggcgtttgg
    44941 ctaggccccg tgcgccatgc agcaatgcgc cggggtacgc tgccttccaa tcgccagcgt
    45001 aaaggtgatg atagatgtta ggggcgatga tgtggcgcac gggcccgagc gcgtcgacct
    45061 cggctttgag gtccggtgtc atggcgagcg gggagtgaac cagaagctgg ccgtcgccaa
    45121 gttggaccac ggtcattcgt gtcccgacat gcagcccata gaaggactgc ggagccgact
    45181 gcgaccaaat ccctgctgcg agctgctgca tacggcggat ctacgacagc gacgtctcga
    45241 aaccaagccg actcgcgtcc atgactggcg ctcacgggga aaactgatac gcctcggcca
    45301 tggctaaccc gacagcaacc ctcgaaacct cgctcggaaa cgtagaaatc gagctcttcg
    45361 ccgacctcat gcccacaacg gcgggcaact tcatcgagct cgcgaagagt gggttctacg
    45421 acgggcttca ctttcaccgc gtcatcaacg gattcatgat ccagtttgga tgcccgcaca
    45481 gccgcgatcc caagagcccg cgcgctggca ccggcgacag ccccaacggc accatccagg
    45541 acgagcaccc ggacaacgca aagctttcga acgaaccggg aacactctcg atggctaaca
    45601 ccggccggcc gaacagcggg agctgccagt tcttcatcaa caccgttcac aacgcttatc
    45661 tggactggtt cagccctgga ccgtccaagc acccggtctt tggcaaagtc gtgagcggca
    45721 tggacgtgat caagaagatc gagaccactc caacgggtgc cggtgaccgg ccggcgacac
    45781 cggtccaaat gatcaaagtt accgttcacg agtagtccga cgcacgacgg gcgaaacgaa
    45841 gcggggctcg acccgggaca ccagggttgg tagatttgaa atcatgacct cgcttcgaat
    45901 ccttctcttc tgcttggcgg cttcgctggg gctcagcgcc tgtacagacg aggtcccgcc
    45961 gcccatgaac ggaacgggag gaagaggcgg gacggcgggc tcgggtggca ttgctggatc
    46021 gggaggctcg gccggcagcg ctgggtccgc cggtttgggc ggcagcgctg gctccgccgg
    46081 ttcgggcgga actgcgggct cgcctggctc tgctgggtcg ggaggagctg cgggctcggg
    46141 tggctccgcc ggttcaggcg gcacaggagg agccgcgggc ggcatgggcg gcggcggcat
    46201 gggcggagcc ctctcgggcg catgcatcaa ccaggacgac ttggccgcct tagcagatct
    46261 tcagccaagc aacgcgcgtg aggtcgcagc cgactgcgga atcgaatgca cggacttaat
    46321 cgccttcgaa caggcattca caacctgtgc gagcgattgc gtcgagcgga tggtaacggg
    46381 cctgtcgtcc ggatgcgcaa cttgctacgg cgagctcgcg tggtgcagca ggttgctctg
    46441 cttgacaccc tgcgcatcgg cttcgtgctt acccgtttgc caagactgca acggatacga
    46501 cctctgcctc gccgagctcg aggcctgcac cggccgagtc tcggtcgatt gcactgaccc
    46561 tacctgagct gcgcctggct acttgccttt gggctgccag cggctgtcgg tgagcgtgag
    46621 cccctcgatc gacttgcgcg ggaccatctg ctcgtacgtg cgcgtgtagc ccgtcttggc
    46681 gatccgccag acgccgtcat cgcccttgcg gtattcgtct tcgtagaacg cggcgccgtg
    46741 aatgatcatg tcgaagctcg tgtcgatgac atagtcctga aggcaccagc gccccgtggc
    46801 cgttcgttcg tcgacgaaat cgatctcggg ctggctcacg acatgactcg agtgaaagct
    46861 gtctgcgccc atcgcgccct ctaggaacct caagatcgcg tcgcggccgt cgaaagagaa
    46921 cttcccgctg ctgtaggccg ccttggcgtc ctcggtgaag cagtcgcgaa gcttttccca
    46981 ctccttggta tcgaggcagc gctgatactt gtatttgagg cgcttgatcg cctcgagatc
    47041 ctgccatttc gcttcaacca tcagcgcaat ctagggcggt cggggcaaga atgccagagt
    47101 cgcgaatccg ctcagagccc ggactcgagg aacgatgggt cacttgcgaa gcgggcgacg
    47161 tggtgctctt cgtcgccaaa cagcgcgttc agtgcgtaca tgcgcttgaa gtagagtccg
    47221 atgtcgtgtt cgtcggttac accgatcccg ccgtgcaact gaagcgattg ctgggccacc
    47281 cagaccccac ccgtcgcgag ctggtacttg gccgcggaaa tgtcggctct cctcgcggcg
    47341 tcgtcgtcat cgtcggcacg aaccatcgaa gccattgaaa tcgagcgcag tagctcggtc
    47401 tctgcgtaca tgtccacggc gcggtgttga agggcttgaa atgatccgat tttcacgccg
    47461 aactgctcgc gggtaccgag gtagtcgacc gtcatctgaa gcatcgtgtt ggcgatgccc
    47521 acgcccgcgg ccaccgccgc cgccgctccg tagtccatgg tccgctcgag aacagcggca
    47581 cccgagcctt ccgcaccaag gcgggcctct tcgttcacgg cgacgccgtc gaactgaacg
    47641 aacgcggctt tttgcccatc gatcgtcttg gcgacggtca ccttcacgcc gttcgctcca
    47701 cgaggaacca cgaagagcgt cacgccgccg ggtgccttgg ccgacacgat cagatggtct
    47761 gcccgatggc cattgagcac ccagaccttc tcgccggaaa gcacgtagcc cttgctgctg
    47821 ggcgttgcac tcgtctcgat cgaccggacg tcgtagcggg tctcagcctc ggagtgcgcg
    47881 agcgcgagcg tgctttcacc ctcgatcatc ggaccgaggt acgcctcttg ctgtgccgcg
    47941 tcgccagcgc gcgcgagtgt catcccgccg agcacgacag aggccagata cggctccgga
    48001 acgagcgtcg tcgcgagatt ctcgagcacg atggcgcatt cgacgaagct cccgccgtag
    48061 ccgcccatcg actccgcgaa ggggactccg agccagccga gctcgcccat cttcttccaa
    48121 acggcagggt cgtaaccgac gtcgtcgtcg cgaagcttgc gaaaccgttc gaccggtgac
    48181 tcgttcttcg cgaaatccgc caccgttttg gccagcatct gttgatcttg gtcgagctca
    48241 aagtccatct cggaatctct cctgtgtcag attcgccggt cagctgctcg gcagctggag
    48301 gatcatcttt gcgattacgt tgcgttgaat ctcgttcgag ccgccataga tagtggccgc
    48361 gcgttcgtag cagaagtagg ggccgatcga ctcctcgatg ggcgggcaaa caccctcgtt
    48421 attccaggtg agcgagctct cccccatgat ctccatcgcg agctcggtca gctcctggat
    48481 cagcttggta ccgaccagct tcaggatcga cgactcagga ccgggcatct ttcctttctg
    48541 ttggtcggcc aaggcccggt agctcaccat caagtgtgcg cggaatcgca tttccagccg
    48601 cgcgatcttg gcgcgccata tcgggtcctc gagcatcggg cgcccgtcga tcttcttcgt
    48661 cgcggcaata cgcttgatct tctggagcac gttacggctc ggtccgatca tcgcgagcat
    48721 cgtgcgctca tggccgagga gcgctttggc catggtccaa ccttggttga tcccgccgac
    48781 tacgttctct ttcggcactt tgacgttgtc gaagaaggtg tcgcagaatg cgggagtgtg
    48841 acccaatgtg agcatcggtt tcacctctac ccctgggctc ttgaggtcga tcagtaagaa
    48901 ggtgatcccc gcttgcttct tcgcggaagg gtccgtgcga accaaacaga agatccagtc
    48961 ggcgtattgt gcatatgtcg tccaggtctt ttgtccgttg acgatgaagt gatcgcctgc
    49021 gtcctcggcc gtcgtccgaa gtgaagcgag atcgctgccg gatcccggtt cggaatatcc
    49081 ctgacaccag agctcctccg cgctcaggat cttgggaagg aaccgcttct tctgtgcgtc
    49141 cgttccgaag ctgatgatca gcggcccgac catagaaagc ccaaatactg acaaaggcgg
    49201 ggcaccagcg agctccatct cttcgttgaa gatgaaacgc ctcgcggcat tccagcccgt
    49261 acctccgacc tccttcggcc agtgtggagc aatccagccc ttcgcatgga gaatccgatg
    49321 ccaaatcttg gagtcctccg gcttgaagcc tcccgcaccg cgcgcttttt ggcgcatgtc
    49381 ctccggcatc gcctcctgaa tccaggcgcg aacctggtgc cgaaacgcct cttcttcctc
    49441 gttgaacgtc aggtccatgg gcgtatgcgt gccacaaaca gcgggcaaat accaacctgt
    49501 acgaacgcag ccaaaccctg taggaagctc agcaccggac ctcagacgtg acgatcgagc
    49561 tcgcattcct cctgttcgcc ctcgtcgtcg tcaccgcata tgcggtccag acggcgaccg
    49621 gattcggggc caccttggtc tcggtgacca tcggcgccca gctcatgggg atcgaggagg
    49681 tcattcgtct gatggttccg ttgtcgttcc tgcagaccgg atacatcgcg atccggcacc
    49741 gcgatggcat cgactggagc ttgctgctca agcgaatcct tccgctgatg gcagtgggga
    49801 tgtctcttgc gtttctgctt ctcacccggg tcggaggtcc ctggctggga ctggcgttcg
    49861 gcttcatcgt tctcgggctc tctggccggg atttgtatct gctccgcgcc gccggcacgg
    49921 cggtcgcaaa accagtcgcg cgcgccgctt ccgtggccgc gattttgagc gcgggtgtga
    49981 ttcacggcat ctatgcttcg ggaggaccga tgcttgtcta tgccgtcggg cgtgaagggc
    50041 tctcgaagaa ggcgtttcgg agcacgatca gcatggtttg gatcgctctc aacctagtgc
    50101 tcgtcgcgcg ttttctcctg gcgggcgact atgatcggaa ggtcgctctg gacgtcctca
    50161 tgctcgtgcc cgccgttccg ctgggaatcc ttctgggtga atgggtgcac caccgagtcg
    50221 acgagcgggc cttcaagatg gcggttctgc tgctcctcat cgtggctgcg gtctcgctga
    50281 tcgtgcgata tagtgcccag ctgctctagc cgcggagaaa ttgaatgtac gacctgaaga
    50341 tcgtcggtgg aacgctagta gacggcaccg gaaccgagcg ccgcacgggt gatgtcggca
    50401 tcaaggacgg gcggatcgtc gacgtcggaa ggtgcgacga aggcgccgcg cgaaaggtcg
    50461 acgccgccgg ggcgatcgtg gcgcccggct tcgtcgacat ccacacacac tacgacggtc
    50521 agatcagctg ggacgaggag cttgcgccgt cgtcgatcca tggggtgacc acctgtgcga
    50581 tggggagctg cggcgtcggc tttgcgcccg tgaggcctgc cgaccacgcc aagctcgtcg
    50641 agctgatgga aggcgtggag gacatcccgg gatcggcgct ctccgaaggc atcagttggg
    50701 gctgggaaag ctttcccgaa tacatggaga cgctcgatcg catgccgcac acgattgatt
    50761 tcctcgcaca cgttccgcac gacgcgctgc gcgtctacgt gatgggcgag cgcgcggtgc
    50821 atgacgaggc ggcaaatgag gacgacatcg ccgggatgcg gcggttgctg cgcgaggcgc
    50881 tagaggccgg ggcgatcgga ttcagcaccg ggcgaagtga caaccatcga agcgcggttg
    50941 gcgactggac gccggcgtcg gaagcggacg gcaaagagct cgcgggcatt gcaaaagctt
    51001 tcgatggctt gagctatggg gtcctgcagg cggtgagtga cttcaacgtc cttcacggag
    51061 atgatcactt cgacgccgag tggggtgtgc tcgagcagat gctcggtgcc gcaggcgggc
    51121 ggccgatgag cgtctcgacg atgcaacgcg accacagccc cgagcaatgg aaatggattc
    51181 tcgacaaagc atccaaggcc accgagaagg gcttcgaggt ccgctgccaa gtcggcgcaa
    51241 gaggaatcgg tgtgatgctc ggtcttcaag cgaccttcca tcccttcatg ggttttccga
    51301 gctacaaggc aatcagccag cttccgctgg ccgagcgggt gaaggccatg cgcgacccag
    51361 ggctcaaggc ccggatgatc gccgagaaga gcgaacccgt ggcgggcgat gggagcatgc
    51421 ttccgccg
//
LOCUS       DASRZF010000106         5672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4890003, whole genome shotgun sequence.
ACCESSION   DASRZF010000106 DASRZF010000000
VERSION     DASRZF010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 5672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5672
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4890003"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..1192
                     /locus_tag="VFG22_04890"
     CDS             191..1192
                     /locus_tag="VFG22_04890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET6415608.1"
                     /translation="MTTSTRAVTVALSVDLVIGTAKVVTFLLTGSTAIMAEMLHSLAD
                     LTNQGLLVIGIARSRRGPDSNYPYGFGRSRYIWALLSAAGVLFVGSGVSVVRGVEQVW
                     APEPLEHLGWGLGVLVVSLVAESISFLFGLSAVRSSAREAKQSVRQYIGIGPDPMGVA
                     VVLEDASAVFGVLLASAGIALAEFTGNPAWDGAASVAIGLLLGASAIFLINRNRRLLL
                     GPAPSSESVARMMSILEGSPVVSKIHDVKVSQLGADAVRFKAEVTFDGRALAKRLLQG
                     TDLDATWSSLTGPEALERLLVEFGSEVTDAIGDEIDRLEAELAEAAPEARHVDLEPD"
     gene            complement(1208..3163)
                     /locus_tag="VFG22_04895"
     CDS             complement(1208..3163)
                     /locus_tag="VFG22_04895"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET6415609.1"
                     /translation="MNHGAHDWKPEQARKSLKRLLPEMEETYGARLESVAFDWHGFKN
                     RLHREWERLFSYLYELYGWQYDFHYTLERILRSLIESWLERPEGLRRLDERREAVPEW
                     FQSQEMVGIVLYVDLFSDQLAKLKDHIPYFKKLGLTYLHLMPLFVSPHGENDGGYAVS
                     DYRSINPDLGTMQELEEVAAVLRDEGISLVLDFVFNHTSDEHRWALRAKSGDPEYMGF
                     YFTFPDRELPDQFQRYLRDIFPEVRAGSFTWNDELQRWVWTTFNSYQWDLNYANAEVF
                     RAMAEEMLFLANRGVEVLRLDAVAFLWKRLGTDCENQPEAHTIIRAFNAMARIAAPAL
                     LFKSEAIVHPNEVIRYVHPDECQLSYNPLLMALLWEALATRDVRLLEHSMRTRFRLPA
                     GCAWVNYLRSHDDIGWTFDDDDARSVGIDPASHRRFLNQFYTGEFPGSYARGKKFQEN
                     ALTGDVRISGTLSSLAGLEEALERNDSELIERAVHRITLLMGIQVSIGGLPLLYAGDE
                     YGMINDYTYLHDPRKVNDSRWVHRCRKRWEAQEDLADLETIEWRFFRETSKLFNLRKR
                     LPALYDGGMEVIETQNPRIFAYLRAHAEQRVLVVTNFSETPERLDLRFLVPWVSGGEA
                     LDHLTGTRLPVADGLVLEHHRAVWLEV"
     gene            complement(3160..5205)
                     /locus_tag="VFG22_04900"
     CDS             complement(3160..5205)
                     /locus_tag="VFG22_04900"
                     /inference="COORDINATES: protein motif:HMM:NF014822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HET6415610.1"
                     /translation="MTNAPTTALIPEEKVQRLIERDPLLKPYQSVLLRRLNHTEAMRR
                     RITDGHLELPELASGHEYFGLHFREGHWILREWAPNATSIHIKGPFSDWRTDPRYALH
                     REGAEGVWEIQLSEAMLAHGDPYRLEVHWPGGAGDRFPAWTRRVVRNEETGSFNAQVW
                     HPSEPYLWRHSRPEHPLGPLLIYETHVGMAQPDPKVGSYEEFRTNVLPRIRDAGYGAV
                     QIMALAEHPYYASFGYQVSSFFACSSRFGTPEELKALVDEAHGMGLRVFMDLVHSHAA
                     RNEVESISAQDGSPSLYFHEGPRGEHPAWNSRCFDYAKPEVLHFLLSNCRFWMDEFRV
                     DGFRFDGVTSMLYLGHGLARVFTSYDDYFGTDVDEDALAYLTLANDLIHELVPGAVTI
                     AEDVSGFPGLALPVAEGGAGFDYRFAMGVADTWIELAKDLPDETWPIGRIWHELTNRR
                     RDESTISYAESHDQALVGDQTLIFRLLGPRMYDGMSVTSEDISVERGIALHKMIRLAT
                     LGTAGHGYLNFMGNEFGHPEWIDFPREGNHWSYAYARRQWPLADDPSLRFHHLAAFDR
                     DLMGLAREYGVPDGQDEFLLHADEGTKVLAWLRGGLVFAMNFHPSRSFTDYQIPSAPG
                     EYRTVLDTDRSAYGGFDRQDHGITHHALADRIQRHFLSLYLPSRTGLVLAPASQASR"
     gene            complement(5249..>5672)
                     /locus_tag="VFG22_04905"
     CDS             complement(5249..>5672)
                     /locus_tag="VFG22_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415611.1"
                     /translation="FHDYQIAYSSRDTPNLRVFREQIEAYKAMLFAAPPDEAQRKDID
                     FLLAGGEIFALVVYGQLILENAKIYDVSDDLVDQIFDFMVRDFSRHALSLHSKPTSND
                     TQMAACMKMIRKPVFDKKRYQRVWEEVHALRDEYEMNP"
BASE COUNT         1087 a         1700 c         1791 g         1094 t
ORIGIN      
        1 ttccaacacg ttgaacgcac gcgaggcttc gaaggagctc cgcgcggtcg tccaacgtcg
       61 tctgtgccca actctgggat gctcgatgag ccctcaggac gagagcctct gcgtcggacg
      121 cgctcattga ggatggtacg ctcatcttct tacgggcgaa tatatcaagg atcgacgcta
      181 tagtactcgg gtgacgacga gcacgcgtgc tgtgacggtc gcgctatcgg tcgatctcgt
      241 gatcggtacc gccaaggtcg tcaccttcct gctgaccgga tcgactgcga tcatggcgga
      301 aatgctccac tccttggcgg acctcacgaa tcagggcctc ttggtgatcg ggatcgcgcg
      361 ctcgcgacgt ggacccgata gcaactatcc gtacggattc ggtcgatcgc gctacatctg
      421 ggcgcttcta tcggccgccg gagtgctctt cgttgggagc ggcgtgtcgg tcgttcgcgg
      481 ggtcgagcaa gtctgggcgc ccgaaccgct ggagcacctc ggctggggct tgggcgtcct
      541 ggtcgtgtcc ctggtggcgg agagcatatc gttcctcttt ggtttgtccg cggttcggag
      601 ctcggcccga gaggcgaagc agtccgtgcg gcagtacatc ggcataggac ccgatccgat
      661 gggggttgcg gtggtgctcg aagatgcgtc agccgtgttc ggcgttctgc tcgcctcggc
      721 gggaatcgcc ttggccgaat tcactgggaa tcccgcatgg gatggtgctg cctccgttgc
      781 catcggactg ctcctcggtg cgtccgcgat ctttctcatc aatcgaaatc ggagactcct
      841 gctcggtccc gcgccttcat cggagtcagt ggctcgaatg atgtcgatac tcgaaggcag
      901 tccggtcgtt tcgaaaatcc acgacgtgaa ggtttcgcag ctgggagcgg acgcggtccg
      961 tttcaaggct gaggtcacct tcgatggtcg cgcgcttgct aagcggctgc tgcaaggaac
     1021 cgatctagat gcgacctggt cttccctgac tgggccggag gccctcgagc gacttctggt
     1081 ggagttcggt agcgaggtca ccgatgcgat cggcgatgaa atcgatcggc tcgaggccga
     1141 gcttgcggag gccgcaccgg aggctcggca cgtggatctc gagccagact gaacgcaccc
     1201 gccgagctca gacttcgagc cagacggcac gatgatgctc gagcaccagg ccatcggcga
     1261 ctggcagccg cgtccccgtc agatggtcca gcgcctcacc gcccgagacc cagggcacga
     1321 gaaagcgcag gtcgagacgt tcaggcgtct ccgagaaatt ggtcacgacc aggactcgct
     1381 gctccgcgtg ggcgcgcagg tacgcgaaga tccgtgggtt ctgggtctcg atgacctcca
     1441 tgccgccgtc gtagagcgcg ggaagcctct tgcggagatt gaagagcttc gaggtctcac
     1501 ggaagaagcg ccactcgatt gtttcgaggt ccgccagatc ttcctgagcc tcccagcgct
     1561 tgcggcagcg atgcacccag cggctgtcgt tcactttgcg ggggtcgtga aggtaggtgt
     1621 agtcgttgat catgccgtac tcatcacccg cgtagagcag cggcaggccg ccgatgctca
     1681 cctgaatgcc catgagcaac gtgattcgat gcacggcacg ctcgatcaac tcgctatcgt
     1741 ttcgctcgag cgcctcctcg agtcccgcga gtgacgagag cgtccccgag atgcgcacat
     1801 ccccggtgag cgcgttctcc tggaacttct tcccacgcgc atacgaccct ggaaattcgc
     1861 ccgtatagaa ctgattcaga aaacgccgat ggctcgccgg atcgatcccc acgcttcggg
     1921 cgtcgtcgtc gtcgaaggtc cagccgatgt cgtcgtggct gcgcaagtag ttgacccagg
     1981 cgcagcccgc cggaagccga aagcgggtgc gcatcgagtg ctcgaggagc ctcacgtccc
     2041 gggtcgcgag cgcctcccac agcagtgcca tgagcaaagg attgtaagaa agctggcact
     2101 cgtccgggtg gacgtaacga atcacctcgt ttggatgcac gatcgcctcg gacttgaaga
     2161 gcagcgccgg ggctgcgatc cgcgccatag cgttgaaagc gcggatgatc gtgtgcgctt
     2221 cgggctggtt ctcgcaatcc gttccgagcc gtttccagag aaacgcgacc gcgtcgagcc
     2281 tcaggacttc gacgccgcga ttcgcgagaa acagcatctc ctcggccatg gcacgaaaca
     2341 cctcggcgtt ggcgtaattc aaatcccatt ggtagctgtt gaacgtcgtc cagacccagc
     2401 gctggagctc gtcgttccag gtgaagctgc ccgcccgcac ctcggggaag atgtcccgga
     2461 ggtagcgctg gaactgatcg ggaagctcgc gatcgggaaa cgtgaaataa aaacccatgt
     2521 actccgggtc gcccgacttc gcccggagcg cccaccggtg ctcgtccgag gtgtggttga
     2581 acacgaagtc taagaccagg ctgatgccct cgtcccgaag gacggctgcg acttcctcga
     2641 gctcctgcat ggtgccaagg tcaggattga tcgagcggta gtcgctcacc gcatagccac
     2701 cgtcgttctc gccgtgggga gaaacgaaga gcggcatcaa atgcagatac gtgaggccga
     2761 gcttcttgaa gtagggaatg tggtccttga gcttggccaa ttgatcgctg aagaggtcga
     2821 cgtaaaggac gataccgacc atctcctgag actgaaacca ctcaggcact gcttcgcgtc
     2881 tttcgtcgag gcggcgtaga ccttcaggac gttcgagcca ggactcgatc agcgaccgaa
     2941 gaatgcgctc gagcgtatag tgaaaatcgt attgccagcc atagagctcg tacagatacg
     3001 agaacaagcg ctcccattcg cgatgaagcc ggttcttgaa accgtgccag tcgaacgcca
     3061 ccgactcgag tcgagcgccg tacgtctctt ccatctcggg gagcaagcgc ttcaaggact
     3121 tgcgagcctg ctcgggcttc caatcgtgag ccccgtggtt catcggctcg cctgggacgc
     3181 tggagcaagc accaggcccg tacgagaagg aagatagagg ctgaggaaat ggcgttggat
     3241 gcgatcggca agcgcatgat gcgtgatccc gtggtcctgc cggtcgaacc ctccgtacgc
     3301 agagcggtcg gtgtcgagga cggtgcggta ctccccaggc gccgatggaa tttgatagtc
     3361 ggtgaaggaa cggctcggat ggaagttcat cgcgaacacc agtccgccgc gaagccaggc
     3421 caggaccttc gtgccttcgt cggcgtggag aagaaactcg tcctgcccgt cgggtacgcc
     3481 gtactcccgc gccaggccca tcaagtcgcg gtcgaacgca gcgaggtggt ggaagcgcag
     3541 gctcgggtca tcggccaagg gccactggcg tcgcgcatag gcataggacc agtggtttcc
     3601 ttcacgtggg aagtcgatcc actcggggtg tccgaactcg tttcccatga aattcagata
     3661 gccgtgcccc gcggttccca aggtggcgag gcggatcatc ttgtgtaacg cgatgccacg
     3721 ctcgacgctg atgtcttcgc tcgtcaccga catgccgtcg tacatgcggg gtccgagcag
     3781 acggaagatc agggtctggt cccctaccag agcctggtcg tgggattcgg cgtagctgat
     3841 cgtcgactcg tcgcgcctgc ggttggtcag ctcgtgccag atcctaccga tcggccaagt
     3901 ctcgtcgggg agatccttgg cgagctcgat ccaggtgtcg gcgacgccca tcgcaaagcg
     3961 gtagtcgaaa cccgcgccac cctctgcaac cggcagcgcc aagccgggaa agcccgagac
     4021 atcctctgcg atcgtgactg cgccgggcac gagctcgtga atcaagtcgt tggcgagggt
     4081 cagatatgcg agcgcatcct cgtcgacgtc tgtaccgaag tagtcgtcat agctggtgaa
     4141 cacacgggcc aagccgtgac cgaggtaaag catactggtg acgccgtcga acctgaagcc
     4201 gtccacgcgg aactcgtcca tccagaaacg acagttggac agaaggaagt gcagaacctc
     4261 gggcttggcg tagtcgaaac agcgcgagtt ccaggcagga tgctcgccgc gcggtccctc
     4321 gtggaagtaa agactcgggc tgccatcttg ggccgaaatg ctctcgacct cgtttcgggc
     4381 agcgtgggaa tgaacgaggt ccatgaacac ccgcagcccc attccatgcg cctcgtccac
     4441 caaagccttg agctcctcgg gcgtcccaaa gcgcgacgaa caggcaaaga agctcgagac
     4501 ttggtatccg aacgaggcgt agtacgggtg ctcggcgaga gccatgatct gcacggcgcc
     4561 gtaacccgca tcgcggatgc gaggcaggac gttcgtccgg aactcttcat agctgccgac
     4621 tttcgggtct ggctgcgcca taccgacgtg agtttcgtag atcagaagcg ggcccaaggg
     4681 atgctccggc ctcgagtgac gccagagata tggctccgac ggatgccaga cctgcgcgtt
     4741 gaagctgccc gtctcttcgt ttcgcaccac gcggcgggtc catgccggaa agcggtcgcc
     4801 tgccccgccc ggccagtgaa cctccagccg ataagggtct ccatgcgcga gcatcgcctc
     4861 ggaaagctga atctcccaaa cgccctctgc accttcgcgg tgaagcgcgt atcggggatc
     4921 cgtgcgccag tcggaaaacg gccccttgat gtggatcgac gtcgcgttcg gggcccactc
     4981 gcgaaggatc cagtggccct cgcgaaaatg cagcccgaag tactcgtgcc cagacgcgag
     5041 ctccggcagt tcgaggtgcc catccgtaat acggcgccgc atggcctcgg tgtgattaag
     5101 ccttcgtaag agcaccgact gatagggctt cagcagcgga tcccgctcga tcagacgctg
     5161 gactttctct tccggaatca gggctgtggt cggggcgtta gtcatacatt ggaggtcctt
     5221 ggccgccgcc gagcccatat cgagaggctc aggggttcat ctcgtattcg tcgcggagcg
     5281 cgtgcacctc ttcccagact cgctggtagc gttttttatc gaagaccggc ttgcgaatca
     5341 tcttcatgca agccgccatc tgtgtgtcgt tgctggtcgg cttcgagtgg agactcaacg
     5401 catggcgcga gaagtcgcgc accatgaagt cgaaaatctg atcgacgagg tcgtcggaga
     5461 cgtcgtagat ctttgcgttc tcgagaatca gctggccata gaccaccagc gcgaagatct
     5521 cgcctcctgc cagcaagaag tcgatgtcct ttcgctgcgc ttcgtctggg ggcgccgcga
     5581 agagcatcgc tttgtaggcc tcgatctgct cgcggaagac acggaggttg ggggtgtcac
     5641 ggctcgagta tgcgatctgg tagtcgtgga aa
//
LOCUS       DASRZF010000107        28148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3514685, whole genome shotgun sequence.
ACCESSION   DASRZF010000107 DASRZF010000000
VERSION     DASRZF010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 28148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28148
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3514685"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1095)
                     /locus_tag="VFG22_04910"
     CDS             complement(<1..1095)
                     /locus_tag="VFG22_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415612.1"
                     /translation="MNAIVQGSDGALNQQPYTGPSGRTRVERVRVFRTGREPAELEVF
                     RVLDVEAYPALRESALNGSLHQLEDGEPVDVPFIYHDPGAFQFVLVIPDGARGRELSE
                     RAKLLDSLMKEHEEDVPDYVRHFAIVHGSKGLATYVSEADTVEVELSELEPVDGPPVI
                     ASYYPRLSGLLPAAGFWTHASTELAPLLEDDELWLFVRVDEDEADAFSEASSDLLVQL
                     KTVEQVPVCVLALTDSRADAVRRAYLNPARSADGRILECLRRDFHATVVVYGAQHRPL
                     RSFRVEAPRAANVKWIIARTELAPQPSPSRWESCVDACRSAPPSVGQIASPFVLAEDA
                     STAAEALDRLRLLEAWSSPARIEEALSVLSV"
     gene            1140..1655
                     /locus_tag="VFG22_04915"
     CDS             1140..1655
                     /locus_tag="VFG22_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415613.1"
                     /translation="MNANTAVMDLTVASVTSVKNATGMELDLSQDTLPILDHYAGLVD
                     APREEILSLLAPMSGAYFGELLRRQLDDGEWVTDSDDYSQWRLRFERCSLTFNPLGIA
                     LEVILGQDIPEWGAHLQTAPEDLLRIKKALDVFGDVRDRDYYTFSVRFEAIEQAYLAL
                     LNDPAGMAPKP"
     gene            1722..2234
                     /locus_tag="VFG22_04920"
     CDS             1722..2234
                     /locus_tag="VFG22_04920"
                     /inference="COORDINATES: protein motif:HMM:NF020839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET6415614.1"
                     /translation="MTDSQAPAIEQPGCGDLLTTGDMARLSESTLRTVRFYEQEGLIE
                     PERRSACGHRLFSERELMKLQLALDLREAGLSVQNIKDLFCLKSDCDCPEEASQRMSR
                     VLNEQIDTMQQKIAKLRKLREELTAMVSVLTECQSCDEGNFPNACDNCDVLGSPELPR
                     ALRVLWHESE"
     gene            2243..2905
                     /locus_tag="VFG22_04925"
     CDS             2243..2905
                     /locus_tag="VFG22_04925"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HET6415615.1"
                     /translation="MEWQPSTPLSAEELARLPVFPLPRVVFFPGTALPLHLFEPRYTE
                     MIEDCLESGCTAMAVALLAPGWESHYEDRPAICEVAGAGRIVAHQRRPDGTHDVILHG
                     LNRVRLHELPDEGRPYRCARAEPIPDAGTASSSDVLAMMACATRVAAKVRERQPDFSL
                     QVSAELSPARAADVVADQLVVSPEERQQILETQDVKERVGLVTQSVASLLAQLSDAHA
                     PS"
     gene            2951..3577
                     /gene="ribA"
                     /locus_tag="VFG22_04930"
     CDS             2951..3577
                     /gene="ribA"
                     /locus_tag="VFG22_04930"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HET6415616.1"
                     /translation="MSGASNREPTIARYAEAEMPSRFGPFRVVVYREHDSDKEHLAVI
                     AGEVEGAEDLLIRVHSECLTGEAFHSLRCDCRDQLDLALERIQAAGNGAVLYLRQEGR
                     GIGLGNKIRAYAKQDEGLDTVDANLALGFEDDERGYQVAADMLRDLGVRSVALMTNNP
                     RKVQGLENDGIEVTRREPHEVEAHEHNRDYLKTKQDRLGHLGNNGDGD"
     gene            complement(3585..5450)
                     /locus_tag="VFG22_04935"
     CDS             complement(3585..5450)
                     /locus_tag="VFG22_04935"
                     /inference="COORDINATES: protein motif:HMM:NF023649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HET6415617.1"
                     /translation="MHRLLKMTLIVAVVVAGCTRAKPENEPELQAPPVQGEPAQATKR
                     PPINRLRNAYFGETHLHTTYSLDAYMGVSRNGPDEAYRFAKGEPMPVPGGTARISAPL
                     DFAAVTDHAEFLSDMYIATHPDNPRYGWEAAIKIRNEERSEQVGEWVFVNVVQNATRS
                     GKPSELSRGALGQTARAGAWKEIQEATERHNNPGVFTTFHAFEWSSAPNGFNLHRNII
                     FRDAVVPDIPVSALDTNEPEKLWAYLAEYEKAGSTLLAIPHNPNYSGDLMFRPKTLAG
                     GPIGVDWARTRARFEPLVEIMQIKQCSETNPAFAPNDEFAAFESFPIDSKRQEGRSNW
                     VREALKDGLRIEKDLGVNPFKLGFVGATDSHNGTPSDIEENDWVGSHGFADGSPKKRL
                     QEELQGWETIRRLNPGAIAGVWAEENTRAAIFDAMARKETFATSGPRIEVRLFAGWDY
                     PDDLDGRSDMVAAAYAGGVPMGGDLSRAPKDKAPKLLIHAVKGPSGANLDRIQVVKGW
                     LGADGNANEKVFDVAWSSGRSMDADGKLPPVGNTVDVSAATYANTIGNATLSTVWTDP
                     EFDPGQRAFYYARVLEIPTPRWSTKDAASLGVAPPVDVPASIQERAWTSPIWYTP"
     gene            5555..6199
                     /locus_tag="VFG22_04940"
     CDS             5555..6199
                     /locus_tag="VFG22_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415618.1"
                     /translation="MNTRRLYAIPIVLVALCAIAACNKDTTETTMPLTYRNPGYEETV
                     FKNLLVIGVSESEEGRRLFENAFAKTLTSDGATATPSYGLLPQSEQLSEEQISKAIQG
                     DGYDGVLITQVLSVDETKQFVQGKSHTVAPRNMGYSGGYYGYYSGGFATVHQPGYFDT
                     HTSFRLETDLYSVANGGLVWSGQSDTMNPKSLESVIDSMTAAVAKELKYEKLIP"
     gene            complement(6196..7269)
                     /gene="prfA"
                     /locus_tag="VFG22_04945"
     CDS             complement(6196..7269)
                     /gene="prfA"
                     /locus_tag="VFG22_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HET6415619.1"
                     /translation="MLPTDKLESLSARYREIEELLCQPNVVSDSKRYTGLTRERAELQ
                     EVVEAYGRYGQVIEDLAGHKEALSDPDLRELAADEIPALESERDQLEAAINLLLLPKD
                     PNDERDTILEIRSGTGGEEAALFASDLFRMYSRYAETQGWKVEVMSLSEASAGGLKEV
                     IASVSGDNVYSRLRFEGGVHRVQRIPATESQGRIHTSTATVAVLPEVDEVDEVKIDPG
                     DLEIMTTAAGGPGGQHVNRTMSAVQIKHIPSGIMVRSEQQRSQHQNKAKAMQILSAKL
                     LDIEREAQASAVSSERRSMVGTGERSEKIRTYNYPQNRITDHRIQLTLHNLDRVMEGE
                     LGDLLTSLRSHHQAELMRQQSSS"
     gene            complement(7272..8285)
                     /locus_tag="VFG22_04950"
     CDS             complement(7272..8285)
                     /locus_tag="VFG22_04950"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HET6415620.1"
                     /translation="MAQPYIGGQAVIEGVMMRAPACLTVAVRRPDGTIAIREGLYRSR
                     WSRKLWKLPGFRGVAMLVESMSMGFGALQFSAEQQMTEEELAQDQGSGRLAVFISTLL
                     ALGLFIALPQGLASVTAGVAGWDIGLTDTSFHFLIGGFKLLIFVGYLFAISRIPDVRR
                     LFQYHGAEHKTIHAYEKQLPLTVANVQSQTTLHPRCGTTFLVVVIVVSIILGSLAAPL
                     LMPDVKGWLGQVALLVIRIGLLPIIAAISYELQRLSARFCTTGWRRFVLYPGFLFQKI
                     TTREPNDDQVEIAIAAMEAAAWREHIQERAPHGEEPIVFASFDRFREVLGGDGSLGAS
                     RAA"
     gene            complement(8322..8570)
                     /gene="rpmE"
                     /locus_tag="VFG22_04955"
     CDS             complement(8322..8570)
                     /gene="rpmE"
                     /locus_tag="VFG22_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HET6415621.1"
                     /translation="MKPDIHPEYNTLTVHCACGASFEMRSTQSIEQVDICSECHPFYT
                     GKQKLVDTAGRVERFRKKYGKSKKDKTAEAAPEAPADN"
     gene            complement(8690..9235)
                     /locus_tag="VFG22_04960"
     CDS             complement(8690..9235)
                     /locus_tag="VFG22_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415622.1"
                     /translation="MGRPNRSIYANARIARAWSIGFYIAGIAALSAGLLVGGIVAVAT
                     ATPVGLSVAAVIAGGGIATGGLSLWESRRFSQRAKRLQSTISEHRLFALAQTRGGVLR
                     VVDVARELTVMGEEAEALLDGLVDEVRVSMQVTDDGEIQYVFRELDGTPSPRVRVAAA
                     HTQTTEESPELNSDSEHELTD"
     gene            9455..11011
                     /locus_tag="VFG22_04965"
     CDS             9455..11011
                     /locus_tag="VFG22_04965"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET6415623.1"
                     /translation="MQAPEPPSQSKVAPKLVVLIVLDQLGSWVLEQQLPMLPSGSFIR
                     RAHESGASLVAAYPFASTQTAPGHASLTTGVAPAVHGIVANAIYDPKLGSRQSVDDGE
                     HAVLGNPERHVSPSRLRSETVADVLHRETGGKARIVGISMKDRSAVLPVGAHPSIAVF
                     YDAVAHSMTTSTYYATKSRLPEWLRDFNKANPVDLLLQTWEPADPGKLEKRLGPDASP
                     GEMYPEFPHDPREAPNPWHAFSATPESSEYLIAAAYAAVKAEQMGLDEVPDFLSLSIS
                     GTDIVGHIWGARSWEYADNLMRIDRALTHFVKLLEARGPVAFVLTADHGVAELPERAH
                     AEGAAGGRLHGSALVAFAEEAVDLELGAGDWIAGYVPPLFTYTSAGKAQRQEISNILR
                     KAMPKLEGVMAVYDVEDAAALRSSRSEIERLVGASLPEPAPGDLYLVMEPGWFDGLSE
                     EGGTNHGSPWDYDRQVPVLMLGAAIQRRTSSEVQDVLQVAGTLAALLDVPAPSGALGH
                     PLPGVMRLPD"
     gene            11025..11285
                     /locus_tag="VFG22_04970"
     CDS             11025..11285
                     /locus_tag="VFG22_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415624.1"
                     /translation="MKLAIAIGKVFVLAGWAWGILSFIAPTEVPRAGIGQMVFLGLLA
                     VHAGEAFAFAKGLAAEDGGSPGGHAWKLLVFGYFHVLGVRYG"
     gene            11323..11601
                     /locus_tag="VFG22_04975"
     CDS             11323..11601
                     /locus_tag="VFG22_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HET6415625.1"
                     /translation="MGQKRIHLVVRGRVQGVYFRATAQREARQHGLSGWVKNRSDGSV
                     EIVAEGEEDDVKDFLSWAHVGPSTARVDAIDVRWRSYTGEYTDFRITR"
     gene            complement(11605..12429)
                     /locus_tag="VFG22_04980"
     CDS             complement(11605..12429)
                     /locus_tag="VFG22_04980"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HET6415626.1"
                     /translation="MIAHLLGLPDTPGVMFLVFAIFGMLYFFLLASVAYYLYFKKFRA
                     RFHPTYELDPKEMKSAIKWVVIGIVGNAALMLPIHMLIANDHSKMYYDVSEHGWGWIP
                     AQIVLLLVFTETLIYWTHWAFHRVPFLWKYVHQRHHVYKVPQPYSALSFNPMDSFLQA
                     VPHHIAAFFFPVHAGVYLASVTFVTLWAVCIHDRVSFFRWPWLNYTDHHTLHHWYSDF
                     NLGQYFTFWDRLVGTYRSPTIDYADVPAGIIYRPPNAPARSTAPSALTNGYAQPAE"
     gene            12670..13656
                     /locus_tag="VFG22_04985"
     CDS             12670..13656
                     /locus_tag="VFG22_04985"
                     /inference="COORDINATES: protein motif:HMM:NF039286.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET6415627.1"
                     /translation="MVENYRFPGIPVGWYTIATSKEVTAGQVQTVKYFGEELILYRTE
                     SGEARLTDAYCPHLGAHLGAGRVEGENLRCAFHGFEFGGDGRCVRTAYGKAVPRKAKL
                     GTWNVREHNGFIFTWYHPEGTQPEWDIPVLPGPAEGWSSFRTRKIEVKSHPQETSENS
                     VDVGHFVEVHGFGGAWYEGDLDVQGHLLKGAYGIKYLIGGKVPLTAKFNVEVHGLGYS
                     LVRVKVPRFDAEINLLVLSSPVDQDNIILRTAASIKRLGPLPVTLLVRELASMGLNKE
                     VGQDAPIWEAKRYVERPILAEGDGPIGEYRRYCKQFYVEKAAKTAQLPVVAA"
     gene            complement(13934..14284)
                     /locus_tag="VFG22_04990"
     CDS             complement(13934..14284)
                     /locus_tag="VFG22_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415628.1"
                     /translation="MADAAIVIRWNRVVPGREKQGLSIFGQSLEYYGTLQKKGAIESF
                     EPVIFNPTGGDLNGLILIRGSADQLDALKREDRFIDLMMRAAHNCEDLGVNDAYIEGE
                     LQARMARWTQIINQ"
     gene            14486..14902
                     /locus_tag="VFG22_04995"
     CDS             14486..14902
                     /locus_tag="VFG22_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415629.1"
                     /translation="MKGSLLLPRIIWASLFGSTLLYVAILELATVQSEGSWPVLLYPL
                     VLAAVSTAGASLLVPRIMLRRRVNGQSAGQEEPNYLTELIVTLALAESVAIFGLVLGF
                     LGAPARVVVPFFAVTWVLMLLRFPTHEKRARFGVEQ"
     gene            complement(14925..15401)
                     /gene="rplQ"
                     /locus_tag="VFG22_05000"
     CDS             complement(14925..15401)
                     /gene="rplQ"
                     /locus_tag="VFG22_05000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HET6415630.1"
                     /translation="MRHQKAGRKFGRNTSHRRAMFRNMAGNLVLHEQIKTTDAKAKEL
                     RRIAERLLTKAIRLGDDLTIDVAKVKDEVERDRILARRLHARRQVARFLPKQLAKTNS
                     DGTIEEVDLIHKLFTELAPRYLERAKADKGGGYTRVIKVNHRRGDNAPMSLIEFIS"
     gene            complement(15434..16456)
                     /locus_tag="VFG22_05005"
     CDS             complement(15434..16456)
                     /locus_tag="VFG22_05005"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HET6415631.1"
                     /translation="MTSPTLVARNWRELIRPKNVPVDQESLSDTYGKFVVEPLERGFG
                     ITLGNSLRRVLLSSLQGAAITAVKIDGALHEFTSVPDVVEDVTEMVLNFKEVVVRSHT
                     ARTQTVRIEKEGPGVVTAGDIKVSDQIEILNPDHKICTLAKGGRFAAELTINVGHGYV
                     PADRNKTAGIPIGTIAIDSLFSPIRKVNYAVTNARVGQITDYDKLTLEVWTDGAVKPA
                     DAVAYAAKILKDQLTIFINFEEHDEPVEESSSEEPLNENLFRSVDELELSVRSANCLQ
                     NANIKLIGELVQKSEGEMLKTKNFGRKSLKEIKEILADMGLSLGMQIDNWSQMLERWK
                     TQQAIQ"
     gene            complement(16475..16876)
                     /gene="rpsK"
                     /locus_tag="VFG22_05010"
     CDS             complement(16475..16876)
                     /gene="rpsK"
                     /locus_tag="VFG22_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HET6415632.1"
                     /translation="MAKAKAKSIKGKKRKTKKVVTSGIAHIQSTFNNTIVTITDLGGE
                     VVSWSTAGARGFKGSRKSTPFAAQLAAEDAARKAQDAGMKTIAIFVKGPGAGRESALR
                     AFQNVGMRVTLLRDVTPIPHNGCRPPKRRRV"
     gene            complement(16881..17249)
                     /gene="rpsM"
                     /locus_tag="VFG22_05015"
     CDS             complement(16881..17249)
                     /gene="rpsM"
                     /locus_tag="VFG22_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HET6415633.1"
                     /translation="MARIAGVDLPGRKHTVIALQYIFGIGPHHAKHICATAGIPESKR
                     ADDLDENDVKKIRECIDLNFKVEGDLRREVNMHIKRLMDLGCYRGLRHRRGLPCRGQR
                     THTNARTRKGPKRSAMSRSR"
     gene            complement(17256..17369)
                     /gene="rpmJ"
                     /locus_tag="VFG22_05020"
     CDS             complement(17256..17369)
                     /gene="rpmJ"
                     /locus_tag="VFG22_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HET6415634.1"
                     /translation="MKVRPSVRKICDKCKIVRRKGVVRVLCENPRHKQRQG"
     gene            complement(17366..17578)
                     /gene="infA"
                     /locus_tag="VFG22_05025"
     CDS             complement(17366..17578)
                     /gene="infA"
                     /locus_tag="VFG22_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HET6415635.1"
                     /translation="MTEARTTRGTIETVLPRALYRVRLGDGVEITASASTKARQVTVK
                     LLPGDDVMVEISPFDPTRGRIKERLK"
     gene            complement(17575..18237)
                     /locus_tag="VFG22_05030"
     CDS             complement(17575..18237)
                     /locus_tag="VFG22_05030"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF001380.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HET6415636.1"
                     /translation="MDLLLFGPPGAGKGTQAKFLIELLGIPQISTGDMMRAERKSGSD
                     LGKQFDEYMSAGKLVPDSLVLDLILKRLTKDDAKNGAIFDGYPRTVAQAESLDALLAK
                     LGRRIDRVVSLDVPLEDIVERVTGRRVCLDCGQTYHVRYTPPPQNGECVSCGGSKIVQ
                     RSDDTEEVVRKRYEEYKAKTEPVLAHYEAKGVVEGVEGVGSLDEITERIKKAIGVEQR
                     AG"
     gene            complement(18237..19562)
                     /gene="secY"
                     /locus_tag="VFG22_05035"
     CDS             complement(18237..19562)
                     /gene="secY"
                     /locus_tag="VFG22_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HET6415637.1"
                     /translation="MSTVANIFRIPELRRRILFTLGMIAVYRIGIFVTTPGVDRTAMR
                     KYVAGQSGGFLSLFNLFSGGALEQASIFALNIMPYVSASIILSLMTVVVPSLMELRKE
                     GEAGQRKINQYTRYGTVGLSIFQSMGLAMLWEGWNESSTAGDIVADPGWSFRLMTVLA
                     LTTGTSFLMWLGEQITERGLGNGISLIIFAGIVANLPDALFMTLEQFKLGELQPIDLM
                     LLIVIALGTTAFIVFFERAQRRLPITYAKRMIGRKMYGGQQSHLPLRVNMAGVIPPIF
                     TSSLLMFPVTLAGTNIPYLSDAMRWISNNLVPGGWVYIVVFATMTVFFCFFYTAITFQ
                     PVDVAENLKKQSAFIPGVRPGKATAEYIDAVLTRITVGGALYVAAVVTLPTLLQARWN
                     VPFYLGGTSLMIVVGVALDFAQQVESHLITRHYEGLTGSKGSRIRARNA"
     gene            complement(19583..20065)
                     /gene="rplO"
                     /locus_tag="VFG22_05040"
     CDS             complement(19583..20065)
                     /gene="rplO"
                     /locus_tag="VFG22_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HET6415638.1"
                     /translation="MSEHSNTPILSRLRPAAGAVRGKRRKGRGPGSGLGKTAGHGQKG
                     QKARHPGNFSKLGFEGGQMPLYRRIPKRGFTNPFTKKVGTVNVKDLARFDAGATVDEA
                     ALREAGLIKRKVDIIKVLGDGELDRALTVRVHAASATATQKIEKAGGTVELVVASTAS
                     "
     gene            complement(20058..20249)
                     /gene="rpmD"
                     /locus_tag="VFG22_05045"
     CDS             complement(20058..20249)
                     /gene="rpmD"
                     /locus_tag="VFG22_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HET6415639.1"
                     /translation="MSASKLRVTQVKSEIGRMEKQRRVLRGLGLRGPNTEVVVENTPA
                     FRGMVKKVLHLVQVEEVNE"
     gene            complement(20261..20848)
                     /gene="rpsE"
                     /locus_tag="VFG22_05050"
     CDS             complement(20261..20848)
                     /gene="rpsE"
                     /locus_tag="VFG22_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HET6415640.1"
                     /translation="MAYKDQIIDPASLDDLTERVIFINRVAKVVKGGRRFSFSALVVV
                     GDSNGHVGVGMGKANEVPESIRKGTDAAKKSIFKVPLTGRTIPHKIVGHSGAGRVVLR
                     PASEGTGVIAGGAMRPVLEAAGVKDVLAKILGTTNAHNVVNATVAALKELESPTDVAR
                     RRSRRKGDIQQFEHGPKAERPAAEPDEAPAEASAS"
     gene            complement(20856..21200)
                     /gene="rplR"
                     /locus_tag="VFG22_05055"
     CDS             complement(20856..21200)
                     /gene="rplR"
                     /locus_tag="VFG22_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_026114014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HET6415641.1"
                     /translation="MQQKLTGRARRKLRIRKKVQGTSERPRLAVFRSTKHIYAQVIDD
                     TQGKTIAHASSVIGSAAGSKVDVAKSVGSAVAKACIDKGVEQVVFDRGGYRYHGRVRA
                     LAESAREAGLKF"
     gene            complement(21203..21799)
                     /gene="rplF"
                     /locus_tag="VFG22_05060"
     CDS             complement(21203..21799)
                     /gene="rplF"
                     /locus_tag="VFG22_05060"
                     /inference="COORDINATES: protein motif:HMM:TIGR03654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HET6415642.1"
                     /translation="MTETQTGKQSRNGKKPVKIADGVKVTISGQNITVKGPKGELTQS
                     LRPEVVVTQDEGKLTVAPAPGAAGKTGLQYQGLTRAILGNMVEGVATGYKRSLDFRGV
                     GYRAEFSNDQLKMTVGLSHQPILDIPKTVSVRVETIDEAGTKFPRVHLESPDKQLVGR
                     IAARIRQTRPAEPYNGKGVRYTGERIREKAGKSGKAGG"
     gene            complement(21799..22191)
                     /gene="rpsH"
                     /locus_tag="VFG22_05065"
     CDS             complement(21799..22191)
                     /gene="rpsH"
                     /locus_tag="VFG22_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HET6415643.1"
                     /translation="MMTDPIADMLTRIRNAAAAQHERTTLPSSNLKAQLAEILKQEGF
                     IDDYRVEDGIQKSLTIFLKYGRDRQSAFVGMRRSSRPGRRFYVGHRDIPRVQNGMGVS
                     ILSTSVGIMTDRDARDKRVGGEVLCEVW"
     gene            complement(22204..22389)
                     /locus_tag="VFG22_05070"
     CDS             complement(22204..22389)
                     /locus_tag="VFG22_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HET6415644.1"
                     /translation="MASKRAFAKLEKTPKFSVRHRNRCKVCGRPRGYYRKFEMCRICL
                     RKYGLSGDIPGLTKSSW"
     gene            complement(22399..22950)
                     /gene="rplE"
                     /locus_tag="VFG22_05075"
     CDS             complement(22399..22950)
                     /gene="rplE"
                     /locus_tag="VFG22_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HET6415645.1"
                     /translation="MSEYEPRLRKKYKDEIIPQLMKEFSFRNVMQVPKLDRVVINMGL
                     GEAVQNAKLIESAVEELTAITGRKPIITRAKKSIATFKLREGMPIGVMVTLRGEQMYD
                     FVDRLISIALPRTRDFKGISPKAFDGRGNYTLGIREQIVFPEINYDKIDRIKGMNVTF
                     VTTAETDEQGRALLKSLGMPFRN"
     gene            complement(22958..23275)
                     /gene="rplX"
                     /locus_tag="VFG22_05080"
     CDS             complement(22958..23275)
                     /gene="rplX"
                     /locus_tag="VFG22_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HET6415646.1"
                     /translation="MTARIKKGDLVQVIAGKDRGEKGRVLQVVRADLILVEGVNRVKR
                     HQSPRRFKEAGIVEKEMPIHASNVMLVDPKTEERTRVRIEADKKDPQKRVRVAVKSGS
                     VLD"
     gene            complement(23275..23643)
                     /gene="rplN"
                     /locus_tag="VFG22_05085"
     CDS             complement(23275..23643)
                     /gene="rplN"
                     /locus_tag="VFG22_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HET6415647.1"
                     /translation="MIQTETQLDVADNSGAKRVACIKVLGGSRRRYAGLGDVIVVSVK
                     EALPTARVKKGEVARAVVVRTKREYQRPDGTYIKFDTNSAVLITKDNEPIGTRIFGPV
                     ARELRAKRFMKIVSLAPEVV"
     gene            complement(23646..23963)
                     /gene="rpsQ"
                     /locus_tag="VFG22_05090"
     CDS             complement(23646..23963)
                     /gene="rpsQ"
                     /locus_tag="VFG22_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HET6415648.1"
                     /translation="MTAAEPGQPTPKATRERGARRRLVGRVVNETQSEGRAQQTVVVE
                     VIRRARDPFYGKYVKRKKKFHAHDEANEYRTNDVVEIRACRPRSKTKRWEVVRLIERP
                     PEV"
     gene            complement(23970..24176)
                     /gene="rpmC"
                     /locus_tag="VFG22_05095"
     CDS             complement(23970..24176)
                     /gene="rpmC"
                     /locus_tag="VFG22_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HET6415649.1"
                     /translation="MKPEDIRANTLEELSVIEGDLRRQLWKARFDNHTNQLDDTSSIQ
                     KLRRDLARVKTILTERRVEAANKE"
     gene            complement(24173..24589)
                     /gene="rplP"
                     /locus_tag="VFG22_05100"
     CDS             complement(24173..24589)
                     /gene="rplP"
                     /locus_tag="VFG22_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HET6415650.1"
                     /translation="MLQPKKTKFRKQMKGRMRGKAYRGGKISFGDFGLQALEPGRITQ
                     RQIEAARMTIQRKVKRQGQLFIRIFPDKPITKKPLETRMGKGKGSVEGWVAVIKPGRM
                     LYEIQGVPEDVAREAFELAAHKLPLKTTFRSRETEL"
     gene            complement(24599..25261)
                     /gene="rpsC"
                     /locus_tag="VFG22_05105"
     CDS             complement(24599..25261)
                     /gene="rpsC"
                     /locus_tag="VFG22_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HET6415651.1"
                     /translation="MGQKVHPFGFRLGVVKTWKSKWYEDKNYAKWLHEDLKLKSAIRK
                     KFAHAGIADIEIERAANKAKVVIYTSRPGIIIGKRGAGVEQLKGDLQSLTQNELFLDI
                     QEVRKAETNAQLVAENIATQLERRVAFRRAMKKAVQTAMKFGVKGIRVTAGGRLGGAE
                     IARTEWYREGRVPLHTLRADIDYGEVTAHTKAGTVGIKCWIFKGEQLPQRPGAGAVGA
                     RV"
     gene            complement(25265..25615)
                     /gene="rplV"
                     /locus_tag="VFG22_05110"
     CDS             complement(25265..25615)
                     /gene="rplV"
                     /locus_tag="VFG22_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HET6415652.1"
                     /translation="MEAVAKARFQRISPRKARIVLNMVRGKSVADALNELRFTTKAAA
                     PIVAKAIGSAIANAKQKNEEINLDALFVKTAFADMAPNQHMRRWRPRAQGRATRVVKG
                     MSHITVVVSDGEES"
     gene            complement(25618..25920)
                     /gene="rpsS"
                     /locus_tag="VFG22_05115"
     CDS             complement(25618..25920)
                     /gene="rpsS"
                     /locus_tag="VFG22_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HET6415653.1"
                     /translation="MARSVKKGPFIDQHLEDKVEAANKSGDKKMIRTWSRRSTVTPDF
                     VGHTFAVHNGRKFVTVFVTENMVGHKLGEFAPTRTFGGHASDKKGGRKINPQTGKK"
     gene            complement(25920..26747)
                     /gene="rplB"
                     /locus_tag="VFG22_05120"
     CDS             complement(25920..26747)
                     /gene="rplB"
                     /locus_tag="VFG22_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HET6415654.1"
                     /translation="MAIRKFKPTSPSRRFYEAPDFDMITRGKPEKKLLESKKQTAGRN
                     SQGRITSRFRGGGHKQRYRRVDFKRNKIGVPATVNAIEYDPNRSARIALVHYADGEKA
                     YILAPDGLQVGQQVLSSRHADVKPGNAMPLRHMPLGTMIHNIELKIGKGAQLVRSAGT
                     AAQLMAKDGDYAQVRLPSGEVRMVHLNCRASVGQVSNSQHARLTLGKAGRSRWLGRRP
                     HNRGTTMNPVDHPMGGGEGRTSGGRHPCSPWGQLAKGLKTRKNKSTNKYIVRGRKKK"
     gene            complement(26750..27040)
                     /gene="rplW"
                     /locus_tag="VFG22_05125"
     CDS             complement(26750..27040)
                     /gene="rplW"
                     /locus_tag="VFG22_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HET6415655.1"
                     /translation="MHVEQVIKRPLYLTEKGAKLREEENKYTFEVDLGANKLQVKDAV
                     ETLFKVTVRDVRTLVMRGHMRRMGKGYAKTQNWKKAIVTLREGETIDFFEGV"
     gene            complement(27040..27663)
                     /gene="rplD"
                     /locus_tag="VFG22_05130"
     CDS             complement(27040..27663)
                     /gene="rplD"
                     /locus_tag="VFG22_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HET6415656.1"
                     /translation="MPKVKVYNLSKKDVGEMELSDSVFDATVSESLLYDVLKAQLASQ
                     RKGAASSQTRAEVSLSKRKIYKQKGTGNARHGSRRAPIFVGGGTVHGPKPRDFSYRPP
                     RKMRLGALKGALSLKLREGQLIVVQDFELSEIKTKKLAEVLDKLEVNGGALIVDAKEN
                     ERLRLSARNLQGHSVLPPEGVNLYDLLRHEHLVLTQTAVQALEARCQ"
     gene            complement(27676..27984)
                     /gene="rpsJ"
                     /locus_tag="VFG22_05135"
     CDS             complement(27676..27984)
                     /gene="rpsJ"
                     /locus_tag="VFG22_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002625389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HET6415657.1"
                     /translation="MAANKIRIRLKAYDHRLLDQSATEIVDTAKRTGARVAGPIPLPT
                     RINKYTVLRGPHVDKKSREQFEIRTHKRLLDILDPTQQTLDALMKLDLSAGVDVEIKS
                     "
     gene            complement(27989..>28148)
                     /gene="tuf"
                     /locus_tag="VFG22_05140"
     CDS             complement(27989..>28148)
                     /gene="tuf"
                     /locus_tag="VFG22_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008426562.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET6415658.1"
                     /translation="ELPEDVKMVMPGDNVKMKVNLITPAAMEEKQRFAIREGGRTIGA
                     GVISKIIE"
BASE COUNT         5221 a         8647 c         8416 g         5864 t
ORIGIN      
        1 cacgctcaag acgctgagtg cctcttcgat gcgagcgggg ctgctccagg cttcgagcag
       61 tcgaagccgg tctagtgcct cggcggccgt cgatgcatcc tcagcgagta cgaacggact
      121 cgcgatctgt ccgacggaag gtggcgccga tcggcaggca tccacgcaag actcccagcg
      181 acttggcgag ggctgcggcg cgagctcggt gcgagcgatg atccacttca cgttcgctgc
      241 gcgtggcgcc tcgactcgga acgaacgcaa gggtcggtgt tgggcgccat agacgacgac
      301 ggttgcgtgg aagtcgcgcc gcaggcactc caggattcga ccgtccgcgg agcgggctgg
      361 gttcagatac gcacgcctca ctgcatcggc gcgcgaatcc gtcagtgcca ggacgcaaac
      421 cggcacctgc tccacggtct ttagttggac caagaggtcg ctgcttgctt ccgagaacgc
      481 gtccgcctcg tcctcatcga cccggacgaa gagccagagc tcgtcgtctt cgagcaaggg
      541 cgccagctcg gtcgacgcat gggtccagaa cccggctgcg ggcagcaggc ccgaaagacg
      601 aggatagtag ctcgcgatga cgggtggccc gtccaccggc tcgagctcgc tgagctccac
      661 ctctaccgtg tcggcctcgg aaacgtaggt cgctagaccc ttcgagccgt gaacgatcgc
      721 gaaatgccgg acgtaatcgg ggacgtcttc ttcgtgctcc ttcatcagcg agtcgagcag
      781 cttggcccgt tcggagagct cgcgaccgcg ggccccgtcc gggatcacca gtacgaactg
      841 gaaggccccc ggatcatggt agatgaaggg gacgtcgacc ggttcgccat cctcgagctg
      901 gtgcaaactg ccgttgagcg ccgattcccg tagcgcaggg tatgcctcca cgtcgaggac
      961 ccggaatacc tccagctcgg caggctcacg gcccgttcga aacacccgaa ctcgttccac
     1021 gcgggtccgg cccgaagggc ctgtataggg ttgctgattc aacgcgccgt ccgagccctg
     1081 aactatcgca ttcatccagc aatggccagt ttgaggaacc aagctattgt gtggtcgcga
     1141 tgaacgccaa caccgccgtg atggacctca ccgtggcatc ggtgacttcg gtcaagaacg
     1201 ccactgggat ggagctcgac ctcagtcagg acacccttcc gatcctcgac cactatgccg
     1261 gcttggtcga cgctcctcgg gaagaaattc tgtcgctcct cgccccaatg agcggcgcct
     1321 acttcggcga gctcctccgt aggcagctcg atgacggcga atgggtgacg gacagcgacg
     1381 attacagcca gtggcggctt cgcttcgagc gttgctcact gactttcaac ccgctgggca
     1441 tcgcgctcga ggtgatcctc ggccaggaca tccccgaatg gggcgcccat ctacagacgg
     1501 cgcccgaaga tctgcttcgc atcaagaagg cgctcgacgt attcggcgac gtgcgagacc
     1561 gcgactacta cacgttttct gtgcgtttcg aggcgatcga acaggcatac ctagctttgc
     1621 tgaatgatcc cgctggcatg gcccccaagc cttgacatgg aacccgcggc ctccatattt
     1681 ctccaagctc ctggaaacac acgcgttttc gagctcctta cgtgaccgac tcacaagcac
     1741 cagcaatcga gcaaccgggc tgcggcgact tactcacaac cggcgacatg gctcggctga
     1801 gtgaaagcac gctgcgcacc gtccgtttct acgagcaaga agggctgatc gagcccgagc
     1861 gtcgaagcgc ttgcggccat cgcctcttct cggagcgtga gttgatgaaa ctccaactgg
     1921 ccctagacct ccgggaagcc ggactctcgg tccagaacat caaggatctc ttttgcctca
     1981 agtccgactg cgactgtccc gaagaagcca gccaacggat gtcacgtgtg ttgaacgagc
     2041 aaatcgacac gatgcaacag aagattgcaa aactccggaa gctgcgagag gagctgaccg
     2101 cgatggtgtc cgtcctgaca gaatgccagt cctgcgacga gggtaatttc cccaacgctt
     2161 gcgacaactg cgatgtgctt ggaagtcccg agctgccacg agctctgcgg gtgctctggc
     2221 acgagtctga atagaggctc gtatggaatg gcaaccaagc actccgctct cggctgaaga
     2281 gctcgcgagg cttccggtat ttccgctgcc gcgcgtggtt tttttcccgg gcacggcgtt
     2341 accgctccat ctgttcgagc cccggtacac cgagatgatc gaagactgcc tcgagagcgg
     2401 ctgcacggcg atggcggtgg ctctgctcgc gcctggctgg gagagccact acgaagaccg
     2461 gcctgcgatc tgcgaagtcg cgggtgcggg tcggatcgtc gcacaccaac gccgccccga
     2521 tgggacccac gacgtcatct tgcacggtct caatcgggtt cgccttcacg agctcccgga
     2581 cgaagggcgt ccgtaccgct gcgcccgcgc ggaacccatc ccggacgcag ggacagccag
     2641 ctcctccgat gtgctcgcga tgatggcctg cgcaacccgc gtggctgcca aggtgcgcga
     2701 gcgacagccc gatttctcct tgcaggtttc agccgagctt tcaccagcgc gtgccgccga
     2761 cgtggtagcc gatcagctcg tggtcagccc ggaagaacga caacaaatcc tggagacgca
     2821 ggacgtcaag gaacgcgtgg gcctcgtgac ccaatcggtg gcctcattgc tcgcgcagct
     2881 ctcggatgca catgcgccgt cctagatcgc atggaatgac ccctcggcct gtggtaccga
     2941 acgttctcac atgagcggag caagcaatcg cgagccgacc atcgcacggt atgccgaggc
     3001 agagatgccc tcgcgctttg gaccgtttcg agtggtcgtg tatcgcgagc acgacagcga
     3061 caaggagcac ctcgccgtga tcgccggtga agtcgagggt gccgaggatt tgctgattcg
     3121 agtgcatagc gagtgcctca ccggggaggc atttcattcg cttcgttgcg attgtcgcga
     3181 ccagctcgac cttgcgctcg aaagaatcca ggctgctggc aacggagccg tgctgtacct
     3241 tcggcaagag gggcgcggca tcggtctcgg caacaagatt cgcgcctacg ccaagcaaga
     3301 cgaaggcctc gacaccgtcg atgcgaatct agctttgggg ttcgaagacg acgagcgcgg
     3361 ctaccaggtt gccgctgaca tgcttcgcga cctcggcgtt cgatcggtgg ccctgatgac
     3421 gaacaaccca cgcaaggtgc agggcctcga gaacgacggc atcgaagtca cccggcgcga
     3481 accgcacgaa gtcgaagcac acgagcacaa ccgcgactat ctcaagacga agcaagatcg
     3541 cttggggcac ttgggcaaca acggcgacgg cgactgagct ccgcctaggg cgtgtaccaa
     3601 atcggcgagg tccaggcgcg ctcttggatg ctggcaggca catcgaccgg gggcgcaacg
     3661 ccgagtgaag cggcatcctt cgtcgaccag cgcggcgtgg ggatctcgag cacacgggcg
     3721 tagtagaacg cccgctgtcc tgggtcgaac tcggggtcgg tccaaaccgt cgagagtgtc
     3781 gcattcccga tcgtgttcgc gtaagtcgcg gcggaaacgt cgaccgtgtt gccaaccggt
     3841 ggaagcttgc cgtcggcatc catgctccgt ccgctcgacc atgcgacgtc gaataccttc
     3901 tcgttcgcgt ttccgtccgc cccgagccat cctttcacga cctgaatccg atcgaggttc
     3961 gcgccactcg gtcccttgac cgcatggatc agaagcttgg gagccttatc cttgggagct
     4021 cggctgaggt ctccgcccat gggaacccct ccggcgtacg ccgcggcaac catgtccgaa
     4081 cggccgtcca gatcgtcggg atagtcccag cctgcaaaca agcgcacctc gatgcgcggc
     4141 ccgctcgtcg cgaacgtttc ttttcgagcc atggcgtcga agatcgcggc tcgggtgttc
     4201 tcttccgccc agacgccggc gatcgctccc ggattgaggc gtcgaatcgt ctcccagcct
     4261 tggagctcct cttggagtcg cttcttcggc gaaccatcgg cgaaaccgtg agagccgacc
     4321 cagtcgttct cctcgatgtc gctcggcgtt ccgttgtgcg agtccgttgc gcccacgaat
     4381 ccgagcttga acgggttcac gccgagatcc ttttcgatcc ggagcccgtc tttcagcgct
     4441 tcgcgcaccc agttggagcg cccttcctgt cgtttcgagt cgatagggaa cgattcgaaa
     4501 gcagcaaact catcgttcgg cgcaaatgcg ggattggtct ccgagcattg cttgatctgc
     4561 atgatttcca ccaggggctc gaagcgtgcg cgtgtgcgcg cccagtccac cccgatgggg
     4621 ccaccggcga gtgtcttcgg gcggaacatc agatcgccgc tgtagttggg gttgtgagga
     4681 atcgccagca gcgttgagcc cgctttctcg tactccgcca gatacgccca gagcttttcc
     4741 ggctcattgg tgtcgagcgc actcacggga atgtctggca cgacggcatc gcgaaagatg
     4801 atgttgcgat gcagattgaa cccattgggt gcagacgacc attcaaaggc atgaaatgtc
     4861 gtgaacaccc cggggttgtt gtgacgctcg gtggcttctt ggatctcctt ccacgcgccg
     4921 gcgcgcgcgg tctgcccaag ggccccacgg ctcagctccg agggcttgcc ggatcgggtg
     4981 gcgttctgaa ccacgttcac gaagacccat tcgccgacct gctcgctccg ttcttcgttg
     5041 cgaatcttga tcgcagcttc ccagccgtat cgcggattgt cgggatgcgt ggcgatgtac
     5101 atgtcactca gaaactccgc gtggtcggtc accgcagcaa agtcgagcgg cgctgaaatg
     5161 cgcgcggtgc ctccgggaac tggcatgggc tcgccctttg caaatcggta tgcctcgtcc
     5221 gggccattgc gggacacccc catgtaagca tcgagcgagt acgtggtgtg gagatgagtc
     5281 tccccgaagt acgcgttacg aagccgattg atagggggac gcttcgtagc ctgggccggc
     5341 tctccttgga caggtggcgc ttgcaattcc ggttcgttct cgggtttggc gcgggtgcag
     5401 ccggcgacca ctacggcaac gatcagcgtc atcttcagca aacgatgcat cagaaaaggg
     5461 aaacgtcatc gtgccgggaa cgtcaagtcc gaatcgcatt gtgtcaccgt aagcgaagct
     5521 ggcgttcggc gtcacgctgc cgtaccctcc gcacgtgaac acacgccggc tctacgcaat
     5581 ccccatcgtc ctcgtcgcgc tttgtgccat cgctgcgtgc aacaaagaca cgaccgagac
     5641 gacaatgccg ttgacgtatc gcaatcctgg ctatgaggaa acggtattca agaaccttct
     5701 ggtcatcgga gtcagcgaga gcgaagaggg gcgacggctg ttcgagaatg cttttgccaa
     5761 gacgctcaca tccgacggcg ccaccgcaac gccgagctac gggctcttgc ctcaaagcga
     5821 gcagctgagc gaagagcaga tcagcaaagc gatccagggc gatggctacg atggtgtgct
     5881 gatcacacaa gtgctgagtg tcgacgaaac aaagcagttc gtacaaggca agagccacac
     5941 agtggcccca agaaacatgg gttactcggg tggctactac gggtactaca gcggaggctt
     6001 cgctaccgtg caccaacccg gctacttcga cacccacact tcctttcggc tcgaaaccga
     6061 cctctactcg gtcgcgaacg gcggcctagt atggtctggc caatccgaca cgatgaatcc
     6121 caaatcgctc gagagcgtga tcgattcgat gaccgccgca gtggcgaaag agctcaagta
     6181 cgaaaagctg atcccctagc tcgaggattg ctgacgcatc agctctgcct gatgatgact
     6241 tctgagcgac gtgagcagat ccccgagctc gccttccatg actcgatcca agttgtgcaa
     6301 cgtcaactga attcggtgat cggtgatgcg gttctgagga tagttgtagg tgcggatctt
     6361 ctcggagcgt tcgcctgttc ccaccatgct gcgtcgctcc gagctgacgg cgctcgcctg
     6421 cgcttcgcgc tcgatgtcga gcagcttggc agaaagaatc tgcatcgcct tggccttgtt
     6481 ctggtgctgc gaccgctgct gttcggagcg caccatgata ccgctcggaa tgtgcttgat
     6541 ctgaactgca gacatcgtgc ggttgacgtg ttggcccccg ggaccgccgg cggcagtcgt
     6601 catgatctcc aaatctcctg gatcgatctt gacctcgtcg acttcgtcga cctcggggag
     6661 gaccgcgacg gtcgccgtgg aggtgtggat ccggccctgt gactcggtcg caggaattcg
     6721 ctgcacgcgg tgcactccac cttcgaagcg gagccgcgag tagacattgt ctcccgaaac
     6781 cgaggcgatg acttccttga gaccgccagc ggaggcctcg ctcaaggaca tgacctcgac
     6841 cttccagccc tgcgtctctg cgtatcgcga atacatgcga aacaaatccg acgcgaagag
     6901 agccgcttct tcaccgccgg ttccgctgcg aatctccagg atcgtatccc gttcgtcgtt
     6961 gggatccttg ggcaggagca ggaggttgat cgcggcctcg agttgatccc tctcgctttc
     7021 gagcgccgga atctcgtcgg ccgcaagctc tcgaagatcc ggatcggaga gcgcctcctt
     7081 gtgtccggcg aggtcctcga tgacctgacc gtatcgtccg taggcttcga ccacttcctg
     7141 gagctccgca cgttccctag tgagcccggt gtagcgcttg gagtctgaca cgacgttggg
     7201 ctggcagagc agctcttcga tctctcggta gcgcgccgat agcgattcga gcttgtcggt
     7261 aggaagcatt ctcaggccgc tcgggaggcg cctaaggagc cgtccccgcc caatacttcc
     7321 ctgaaccggt cgaagctcgc aaagacaatc ggctcttcgc cgtgcggcgc ccgctcttgg
     7381 atgtgttccc gccaggcagc ggcctccatg gcggcgatgg cgatctcgac ttggtcgtcg
     7441 ttcggttcac gcgtggtgat cttctgaaag agaaagcccg ggtacaagac gaaacgccgc
     7501 cagcccgtag tgcagaatcg cgcgctgagc cgctgcagct cgtaggaaat agcggcgatg
     7561 atcgggagaa gacctattcg aatgactagc agcgccacct ggccgagcca gcccttcacg
     7621 tcgggcatca ggagcggcgc agccagcgag cccagaatga tcgaaacgac gatcaccacg
     7681 acgaggaacg tcgtgccgca cctcgggtgt agcgtggtct gtgactgcac gttcgcgacg
     7741 gtgagcggaa gctgcttctc ataggcgtga atcgtcttgt gctccgcgcc gtgatattgg
     7801 aacaggcgcc gcacgtccgg gattcggctg atcgcaaata gatagcccac gaagatcagt
     7861 agcttgaacc cgccgatcaa gaagtgaaaa ctggtatcgg tgagtccgat gtcccaacca
     7921 gctactcctg cggtgacgct cgcgaggccc tgtggcaacg cgatgaaaag accaagcgcg
     7981 agcagcgtcg aaatgaacac ggccaaacgc cccgacccct ggtcttgcgc gagctcctcc
     8041 tccgtcatct gctgctcagc ggagaattgg agcgcgccaa agcccatgga catcgactcg
     8101 accagcatgg ctacgccgcg aaagccggga agcttccaaa gctttctgga ccaacgagaa
     8161 cgatagaggc cttcgcgaat cgcgatcgtc ccatcaggtc gacgaacggc aaccgtgagg
     8221 cacgccggcg cgcgcatcat cacgccttca atgacggcct gcccaccgat gtacggctgc
     8281 gccatcgtgg ccagtatggt gcccttgcgg ggcaaggcag cctagttgtc ggcgggcgcc
     8341 tcgggcgccg cctcagccgt cttgtccttt ttcgacttcc cgtacttctt ccggaaccgc
     8401 tcgactctac cggcggtgtc gaccagcttt tgcttgcccg tgtagaacgg atggcattcc
     8461 gagcagatgt ccacctgttc gatgctctgg gtcgagcgca tctcgaacga agcaccgcag
     8521 gcgcagtgca cagtcaaggt attgtattca ggatggatat cgggtttcat atcagtcgtg
     8581 ctccgtcgct gagcgaggtc ggcgtatgta gcccagccaa cccccggcag caaatcatcg
     8641 ggcccatcga ctgggagtgc aaggcgcccc gcccaataga gctgtgattt caatctgtta
     8701 gctcatgctc ggagtcactg ttgagctcgg ggctctcttc ggtcgtttgg gtatgcgcgg
     8761 cagcaacccg aacgcgtggg ctcggcgtcc cgtccagctc gcgaaagacg tattggattt
     8821 cgccgtcgtc ggtgacttgc atcgagacgc gcacctcgtc gacgaggcca tcgagcaagg
     8881 cctcggcctc ttcgcccatc accgtcaatt ctcgcgccac gtccaccaca cgcaggaccc
     8941 ccccgcgcgt ttgtgcaagg gcgaaaaggc gatgctcgga gatcgtgctc tgtaggcgct
     9001 tggcgcgctg gctgaaccgg cggctttccc aaaggctcag gccgccagtg gcgatgcctc
     9061 ctcctgcgat gacagcagcc acgctgagac ccactggcgt cgcagtggcg actgcaacga
     9121 tgcctccaac gagcaagccg gcactcaatg ccgcaattcc ggcgatgtag aacccgatcg
     9181 accaagctcg ggcgatgcga gcattggcat agatgcttcg atttggtcgg cccatgtaag
     9241 agagcttctt tagcgccctc cacccccaag cttcaagcct cgttgttcta gaccgctagc
     9301 ggtgggcctt tccccacgtt aggttgcgcc cgtgacgatg aattcgaccg ggctcgcgcg
     9361 gttggtccta gcaatagctt tcgcggccct ttcgtttagc gtggccggct gcgcgacgac
     9421 gaagccccag gacgaacccc ttgtggaaaa ggcggtgcaa gcacccgagc cgccgtccca
     9481 gtccaaggtc gcgccaaagc tcgtggtgct catcgtgctc gaccaactgg ggtcttgggt
     9541 gctcgaacag caactgccga tgctgccgag cggtagcttc attcgacgcg cgcacgaatc
     9601 cggtgcgtcg cttgtggccg cgtatccctt cgcctccacg caaaccgcgc cgggccacgc
     9661 atcgctgacc acgggcgttg cgcccgcagt gcatggaatc gtcgctaacg ctatctacga
     9721 tccaaagctt ggatcccgac agtccgtcga cgacggcgag cacgccgttc taggcaatcc
     9781 agagcgccat gtgagcccca gccgacttcg ctctgaaacg gtggccgacg tgttgcatcg
     9841 cgagaccggc ggaaaggcgc gcatcgtcgg tatctcgatg aaggaccgct ctgcagtgct
     9901 gccagttggg gcgcatccga gcatcgcggt cttctatgat gcggtggcgc actcgatgac
     9961 cacttccacg tactacgcga cgaaaagcag gctgccggaa tggcttcgtg acttcaacaa
    10021 ggccaacccg gtggacctcc tgctgcaaac ctgggagccc gcggatccgg gcaagctcga
    10081 gaagcgactg ggccccgacg catcgccagg cgagatgtac ccggagtttc cgcatgaccc
    10141 gcgcgaagcg ccgaatccct ggcacgcctt ctctgcgact cccgagtcga gcgagtactt
    10201 gatcgctgcc gcctacgcag cggtgaaagc ggaacaaatg ggtctcgacg aagttccgga
    10261 cttcttgtcg ctgagcattt cgggtaccga catcgtaggg cacatctggg gcgcccgttc
    10321 ctgggagtac gccgacaacc tcatgcggat cgatcgggcg ctcacccatt tcgtgaagct
    10381 cttggaagcg cgcggacccg tcgccttcgt actgacggca gaccacggtg tcgcggagtt
    10441 gcccgagcgg gctcacgcag aaggagccgc gggggggcgt cttcatgggt ctgctctagt
    10501 cgcctttgcc gaggaagcgg tggacttgga gctgggcgcg ggagactgga tcgccggcta
    10561 cgttccgccc ttgttcacgt acacaagcgc cggcaaagcg cagcggcagg agatcagcaa
    10621 catccttcgc aaagccatgc cgaaactcga gggggtaatg gccgtctacg acgtggagga
    10681 cgcagctgcg ctgcgctcga gccgttcaga gatcgagcgg ctcgtcggcg cgagtcttcc
    10741 agagccggcc ccgggcgacc tttatctggt gatggagcct ggctggttcg acgggctctc
    10801 cgaagaaggc ggaaccaacc acgggagccc ctgggactac gaccgccaag tgcccgtcct
    10861 catgctgggg gccgcgatcc aacgtcgtac gagctccgag gtacaggacg tgctgcaggt
    10921 tgcagggacg ctcgcagcgc ttcttgacgt tcccgctcca agcggggctc tgggccatcc
    10981 actgccgggt gtcatgcgac tgccagattg agtatgctct cgacatgaag cttgcaatcg
    11041 ccattggtaa agtcttcgta ctcgccggat gggcttgggg cattctctcg ttcattgcac
    11101 cgactgaagt tccgcgagca gggatcggcc agatggtctt cctaggtttg ttggcggtgc
    11161 atgccggaga agccttcgct ttcgccaagg gcctggcggc cgaagatggc ggttcgcccg
    11221 gcggccacgc ttggaagctg ctcgttttcg ggtacttcca cgtacttgga gtgcgatacg
    11281 gctgaggttt gcggagccga atcgatgggc aataactgag cgatgggcca gaaacgaatt
    11341 cacctcgtcg tccgcggccg tgtccaaggc gtgtacttca gagccaccgc gcagcgcgag
    11401 gcgcggcagc acggtttgag cggttgggtg aagaaccgca gtgatggctc ggtcgagatc
    11461 gtcgccgagg gcgaagagga tgacgtgaag gacttcctgt cctgggcaca cgtcggccct
    11521 tcgaccgcac gcgtcgatgc catcgacgta cgttggcgaa gctacacggg cgaatacaca
    11581 gattttcgaa tcacgcgctg agggttactc ggcgggttgc gcgtagccgt tcgtgagcgc
    11641 gctcggcgcg gtggagcgcg caggagcgtt cggcggacgg tagatgattc cggcgggcac
    11701 gtcggcgtag tcgatcgtcg ggcttcgata ggtgcctacg agtctgtccc agaaagtgaa
    11761 gtactggccc agattgaagt cactgtacca atggtgaagc gtatggtggt cggtgtagtt
    11821 cagccagggc cagcgaaaga agctcacgcg atcgtgaatg cacaccgccc aaagcgtgac
    11881 gaaggtgacg gacgcaagat agacgccggc gtgcaccgga aagaaaaacg ccgcaatgtg
    11941 gtgcggaacc gcctgcagga acgagtccat aggattgaat gaaagggccg aatagggctg
    12001 cggcaccttg tacacgtggt gccgctggtg gacgtacttc caaagaaaag gcacgcgatg
    12061 gaacgcccag tgcgtccaat agatcagcgt ttcggtgaat acgagcagaa gcacgatctg
    12121 agccgggatc cagccccagc cgtgctcgct cacgtcgtag tacatcttcg agtggtcgtt
    12181 cgcgatcagc atgtggatcg ggagcatgag ggcggcgttg cccacgatgc cgatgacgac
    12241 ccacttgatc gcggatttca tctctttcgg gtcgagctcg taggtcggat gaaagcgggc
    12301 acgaaacttc ttgaaataga ggtagtacgc cacggatgcg agcaggaaga agtagagcat
    12361 cccgaagatg gcaaacacga gaaacatcac accgggtgtg tccggcaggc ctaataagtg
    12421 tgcgatcaca gccgctttaa tatcggcttc gcagggcttg taaagagact ggcaactgag
    12481 ccctcgacgc agataggcct ataatcaaag gccttttgtc ccttggtctg ctttcgggaa
    12541 cggctcgccc gggagagcca accgttcacc ctctgcccgg taccgttcac ccggtttttc
    12601 ggtctctcag gggcgatgag cctacattcc cgggcgagat ggggtttggc cccgttcagg
    12661 agaggaacct tggtcgagaa ctaccgattc cctggcattc ccgttggttg gtacaccatc
    12721 gcgacctcga aagaggtcac cgctggtcag gtccagacgg tgaagtactt tggtgaagaa
    12781 ctgattctct accgaaccga atcgggggag gctagactca ccgacgctta ctgcccccac
    12841 ctcggagcac acctcggcgc ggggcgagtc gagggagaaa accttcgctg cgccttccac
    12901 ggcttcgagt tcggcggcga cggtcgctgc gtacgtacgg cgtacggcaa ggcggtgccc
    12961 cgcaaagcaa agctcggcac ctggaatgta cgcgagcaca acggcttcat cttcacctgg
    13021 tatcacccag agggaacgca gcctgagtgg gacattccag tcctgccggg gccggccgag
    13081 ggctggagca gctttcgtac gcgaaagatc gaagtcaaaa gccatccgca ggaaacgagc
    13141 gagaacagcg tcgacgtagg gcacttcgtc gaggttcacg gtttcggagg cgcctggtac
    13201 gagggagatc tcgacgtgca ggggcatctg ctcaagggcg cgtacgggat caagtacctg
    13261 atcggtggca aggttccgct caccgcgaag ttcaacgtgg aagtccacgg actcggctac
    13321 tcgctcgtcc gcgtgaaggt cccgcgtttc gacgcagaga tcaacttgct cgtgctgtcg
    13381 agcccagtcg accaagacaa catcattttg cggaccgcgg cctcgatcaa gcgcctaggc
    13441 cctctcccag tcacgctgct cgttcgcgag ctcgcttcga tgggcctcaa caaagaggtt
    13501 ggccaggacg cgccgatttg ggaagccaag cgctacgtcg aaaggcccat cctcgccgaa
    13561 ggcgacggcc caatcggcga gtaccgccgc tattgcaagc agttctacgt ggagaaagcg
    13621 gcaaaaaccg cacagctccc cgtagtcgcc gcctgaaccg gaaccggatc actggcgctg
    13681 gcactggcac tggcactggt cagggtgctg gaacttgaag ccgacgctgc cgtcaaactg
    13741 tggaggggcg cgggggaacg cgatgcgggg gttcggcggc gtgtgagtgc caatcggtca
    13801 gcgagcagcc ccctccgatt ggcgcgagct ccgaggggcg ccttgcgtgg cccctgctcg
    13861 ccatccttcc ggccaccttt gcaaggcgcc ccgtccgttg aacccccgca tcgcgttccc
    13921 ccgcgccccg ttcttactgg ttgatgatct gcgtccagcg cgccatgcgc gcttggagct
    13981 ctccttcgat ataggcgtcg ttcacaccga ggtcttcgca gttgtgagcc gcgcgcatca
    14041 tgagatcgat gaaccgatct tcgcgcttga gtgcatcgag ttggtctgcg cttccgcgga
    14101 tgaggatgag cccgttgagg tcgccccccg tcgggttgaa aatcaccggc tcgaagctct
    14161 cgatggcacc ctttttttgc aaggtgccgt agtactctaa agactgacca aagatcgaaa
    14221 gaccctgctt ctcgcgtcct ggtacgacgc gattccatcg aattacaatt gctgcatcag
    14281 ccatggtcct tctcctttgc aaaggagagc gtccgccgct cgccttgaat cgatgacggt
    14341 acgcctagaa caaagacttg ctctatacgc aattgttctc agtcacgtcc cggattaggg
    14401 ctccacgaaa atggtctcgg cgtgacactg acagcccggg agcgcccact tggtggacgg
    14461 aatcgccgtc tgccatgatg aaggcatgaa aggatctctc cttcttcccc gaatcatctg
    14521 ggcttccctg tttggctcta cgctgctcta cgtcgcgatt ctggagctcg cgaccgtgca
    14581 gtccgaggga agttggccgg tgctcttgta cccgctggtt ctcgcagcag tttccaccgc
    14641 gggggcgagc ttgctagtgc cacgcatcat gctgaggcga cgagtgaacg gtcaatcggc
    14701 agggcaggaa gaacccaact acctcacgga gttgattgtc accctggccc tggccgaaag
    14761 cgtcgcgatc tttggcctcg tcctcggatt tctcggggcg ccggcacgag tcgtggtgcc
    14821 cttcttcgcc gttacctggg tcctcatgct cctccgcttc ccaacccacg agaagcgggc
    14881 ccgtttcggc gtcgagcagt gatcgagctc ggtccctggg acctctagct gatgaactcg
    14941 atcaaggaca tcggggcgtt gtcgccacgg cgatggttga ccttgatgac tctggtgtag
    15001 ccgccgccct tgtcggcctt ggctcgttcc aagtaacggg gtgcaagctc ggtgaagagc
    15061 ttgtgaatga ggtccacctc ttcgatggtg ccatcggaat tggtcttggc gagctgcttc
    15121 ggcaggaagc gagccacctg gcggcgcgcg tggaggcggc gagcaagaat cctatctcgc
    15181 tccacctcgt ctttgacctt cgcaacgtcg atggtgagat cgtccccgag ccgaatcgcc
    15241 ttggtgagca gtcgctcagc gatgcgccga agctctttcg ccttggcgtc cgtcgtcttg
    15301 atctgttcgt gcaaaaccag attgcccgcc atgttcctga acatggctcg gcgatggctg
    15361 gtgttacgac cgaactttct gcctgccttt tgatgcctca tgacgaccta cccgcgaccg
    15421 aagtccaaat acgttattga atggcctgct gtgtcttcca gcgctcgagc atctgcgacc
    15481 agttgtcgat ctgcataccg agggagagac ccatatcagc caggatttcc ttgatttcct
    15541 tgagcgactt gcgcccgaag ttcttggtct tgagcatctc gccttcgctc ttctgcacca
    15601 actcgccaat caacttgatg ttggcgttct ggaggcagtt cgccgagcgc accgagagct
    15661 cgagctcgtc gaccgagcgg aacaggttct cgttgagcgg ctcctcggag ctcgactctt
    15721 caaccggctc atcgtgctct tcgaaattga tgaagatcgt gagctggtcc ttgagaatct
    15781 ttgccgcata agcgaccgca tcggcaggct tcacagcacc gtcggtccat acctcgagcg
    15841 tgagcttgtc gtagtccgtg atctgaccca cacgagcatt cgtcaccgca tagttgacct
    15901 tgcgaatggg cgaaaatagc gagtcgatag cgatcgtacc gattggaata ccggccgtct
    15961 tgttgcgatc cgcggggacg tagccatggc ccacgttgat cgtgagctcc gccgcgaaac
    16021 ggccaccttt cgccagagtg caaatcttgt gatcgggatt gaggatctcg atttggtcgc
    16081 tgaccttgat gtcaccggcc gtaacgactc cgggaccttc cttctcgata cggacggtct
    16141 gtgtacgcgc cgtgtgcgag cggactacca cttccttgaa gttgaggacc atctccgtca
    16201 cgtcctcgac cacgtctggt acggacgtga attcatggag cgcaccgtcg atcttgacgg
    16261 cggtgattgc agcgccttgg agagaggaca gcaacacgcg acgcagcgag ttgccgaggg
    16321 tgattccaaa acctcgctcg agcggctcca cgacgaactt gccgtaggtg tcactcagcg
    16381 attcttgatc aaccggaacg ttcttcgggc gaatgagttc tcgccagttg cgcgcgacca
    16441 gagtcggact cgtcatcaat tgcctccgca gcctttaaac gcgccgccgc tttggcggcc
    16501 ggcaaccatt gtgtggaatt ggtgtcacgt cgcgaagcag ggtgacccgc atcccgacgt
    16561 tctgaaatgc tcgaagtgcg gactcgcggc ctgcaccggg acccttcacg aaaatcgcga
    16621 tggtcttcat accagcgtcc tgcgctttgc gggccgcatc ttctgctgcg agctgtgccg
    16681 caaagggcgt gctcttgcgc gagcccttga agccacgcgc ccctgccgtc gaccaggaca
    16741 ccacctcgcc acctagatcg gtaatggtga cgatcgtgtt gttgaaggtc gactgaatgt
    16801 gagcgatgcc gctcgtgacg accttcttgg tcttacgctt cttgcccttg atggactttg
    16861 ctttcgcctt cgccatgttc ttatcggctc ctgctcattg cgctgcgctt cggacccttg
    16921 cgggtgcggg cgttggtgtg ggtccgttga ccgcgacaag gcaggccgcg ccgatggcga
    16981 agcccacgat agcaaccgag gtccatgagc ctcttgatgt gcatgttgac ctcgcgccgg
    17041 aggtcaccct cgaccttgaa attcaggtcg atgcactcgc ggatcttctt gacgtcgttt
    17101 tcgtcgagat cgtcggctcg cttgctctcc gggatacccg ccgtcgcgca aatgtgcttc
    17161 gcgtgatgcg gaccgatccc gaagatgtat tgcaacgcaa tcaccgtatg tttccgacct
    17221 gggaggtcga cgcctgcgat acgtgccatt cgcttctatc cttgccgttg cttatgacgg
    17281 gggttttcac acaagacacg cacgacaccc ttgcggcgca cgatcttgca cttgtcacaa
    17341 atttttctga cgcttggtcg aaccttcatt tcagcctctc tttgattcgt cctctcgtcg
    17401 ggtcaaaagg ggagatttcc accatcacat cgtccccggg caggagtttc accgtgacct
    17461 gtctggcctt tgtcgacgcc gacgcggtga tctctacacc gtcgccgagt ctcactcggt
    17521 aaagcgccct cggtagcacc gtttcgatgg tgcctcgggt cgttctggct tcagtcatcc
    17581 cgctcgttgt tcaaccccaa ttgccttctt gatacgttcc gtaatctcat ctaatgatcc
    17641 gacgccctcc acaccctcca cgacgccctt ggcttcgtag tgggcgagta ctggctcggt
    17701 cttggctttg tactcttcgt accgcttccg gacgacctct tccgtgtcgt ccgaacgctg
    17761 cacaatcttg ctccccccac aggagacaca ctctccgttt tggggcggcg gagtatagcg
    17821 gacgtggtag gtctgtccac agtccaaaca gaccctcctg cctgtgaccc gttctacgat
    17881 atcctcgagc ggaacatcga gggaaacgac ccggtcgatc cgccggccaa gctttgcgag
    17941 gagagcgtcg agtgactccg cttgagccac ggttcgcgga taaccatcga agatcgcacc
    18001 gtttttagca tcgtccttgg tgagtcgctt caggatgaga tccaggacaa gactgtcggg
    18061 caccaatttt ccagcggaca tgtactcgtc gaactgcttt cccagatccg agccagactt
    18121 ccgctcagcg cgcatcatgt cgccagtcga gatctgagga atacccagaa gctcgatcag
    18181 gaacttggcc tgagtgccct tgccagcgcc gggaggtccg aaaagaagca ggtccatcac
    18241 gcgttcctcg ctcgaatccg cgagcccttg ctcccagtga ggccttcgta gtgccgagtg
    18301 atgagatggc tctcgacctg ctgcgcaaag tcgagcgcga caccgacgac gatcatcagc
    18361 gaagtgccac cgagatagaa cggcacgttc cagcgcgcct gaagcaacgt gggcagcgtg
    18421 acgacggccg ccacgtaaag agcaccgcca acggtaattc gagtcagcac cgcgtcgatg
    18481 tactccgccg tagccttacc gggtcgaaca ccgggaatga aagcgctttg cttcttgagg
    18541 ttctcggcaa cgtcgaccgg ttggaacgtt atcgccgtat agaagaaaca gaagaacacg
    18601 gtcatcgtcg cgaagacgac gatgtagacc catccgcctg ggaccaggtt gttgctgatc
    18661 catcgcatgg catccgacaa atacggaatg ttcgttccgg ccaaggtgac cggaaacatc
    18721 aacaaggatg aagtgaagat cggcgggatg acgccagcca tgttcacgcg aagcggcagg
    18781 tgagactgct gtcctccgta catcttgcgc ccgatcatgc gctttgcgta cgtgatcggc
    18841 aggcgcctct gagctcgttc gaaaaagacg atgaacgccg tggttccgag cgcgatgacg
    18901 atcaacagca tcaggtcgat gggctgaagc tctccgagct tgaactgctc caaagtcatg
    18961 aagagagcgt cgggcaaatt ggccacgata ccggcgaaaa tgatgaggga aatgccgttt
    19021 ccgaggccac gctcggtgat ctgctcgcca agccacatca ggaacgacgt gccggtggtc
    19081 agcgcgagca cggtcatcaa gcggaagctc caacccgggt cggcgacgat atcaccggcg
    19141 gtggagctct cgttccagcc ttcccagagc atcgcgagtc ccatgctctg gaagatgctg
    19201 agaccgacgg tgccgtaacg ggtgtactga ttgatcttcc gctggcccgc ttcaccttcc
    19261 ttgcgaagct ccatgagcga aggcacgacg accgtcatca gcgacaagat gatgctcgcc
    19321 gaaacgtaag gcatgatgtt caacgcgaaa atcgacgcct gctcgagcgc accacctgag
    19381 aacaggttga agaggctcaa gaagccaccc gactgaccag ctacgtactt acgcatggcc
    19441 gtccggtcga cgccgggggt cgttacgaaa atcccgatgc ggtagaccgc gatcattccc
    19501 aacgtgaaga gaatgcggcg gcggagctcg gggattcgaa agatgttcgc gacggtgctc
    19561 atggcttgct acgggctttc ggttaggagg cggtcgatgc gacgactagc tcgaccgtac
    19621 cgcccgcttt ctcgatcttc tgcgtggcag tcgcgctagc tgcgtggact ctgacggtga
    19681 gagctctgtc tagctcgcca tcgccgagaa ccttaatgat gtccacttta cgcttgatga
    19741 ggccagcttc tcgcagggcc gcctcgtcga ccgtggcccc ggcatcgaat cgagcgaggt
    19801 ccttcacatt gacggttccg accttcttgg tgaaggggtt ggtgaagccg cgcttgggaa
    19861 tacgccgata gagcggcatc tgcccgccct cgaacccgag cttgctgaag ttaccgggat
    19921 gcctcgcctt ttgacccttc tggccgtgac cagcggtctt gcccaagccg gagccggggc
    19981 cgcgtccttt gcgtcgcttt cctcggaccg cacccgctgc agggcggagc cgggaaagga
    20041 ttggcgtgtt gctatgctca ctcattgact tcctcaactt ggacgagatg gagcaccttc
    20101 ttgaccattc cgcggaaggc cggggtgttc tcgacaacga cctccgtgtt tggtccacgc
    20161 aggccgaggc cccgaaggac tcgccgttgt ttctccatcc ggccaatctc gctcttgacc
    20221 tgggtcactc tgagtttact cgcgctcatg actgcctctc ttacgatgcg ctcgcctccg
    20281 cgggcgcttc gtcaggctcg gcggcgggac gttccgcctt gggcccatgc tcgaactgtt
    20341 gaatgtcgcc cttgcgacgg ctgcggcggc gagccacgtc cgtcggcgac tctagctctt
    20401 tgagtgccgc aactgtggcg ttgaccacgt tgtgggcatt cgtggtgccg aggatcttcg
    20461 ccaagacatc cttgacgccc gccgcctcga gaaccggtct catcgcgcca ccggcgatga
    20521 cgccagtgcc ttcggatgcc gggcgcagaa cgacgcggcc cgcaccggaa tgacccacga
    20581 tcttgtgtgg aatcgttcgc ccggtcagcg gaaccttgaa gatgctcttc ttcgccgcgt
    20641 cggtaccctt gcgaatcgac tcggggacct cgttggcctt acccattccg acgcccacat
    20701 ggccgttgct gtcacccacg acaaccaggg cgctgaaact gaagcgccgg ccgcccttga
    20761 ccaccttggc cacgcgattg atgaagatca cgcgctccgt gaggtcgtct agcgaagctg
    20821 gatcgatgat ttggtctttg tatgccatct ggtcctcaga acttcagacc cgcttcgcgc
    20881 gccgattcgg cgagtgcgcg aacgcgtcca tgatatcgat aaccgccacg gtcgaagacg
    20941 acctgctcga cccctttgtc gatgcatgcc ttggccacag cgctgccgac cgacttggcg
    21001 acgtcgactt tgctccctgc ggcagatccg atgacgctcg acgcatgggc aatggtcttg
    21061 ccctgcgtat cgtcgatgac ctgcgcatag atgtgcttcg tgctccggaa aacggcgagc
    21121 cggggccgct cggaggtgcc ttgcaccttc ttgcggattc gcagcttgcg ccgagcacgt
    21181 cctgtgagtt tctgctgcat gatcagcctc cagcctttcc ggacttaccg gccttctcac
    21241 gaatgcgctc gcctgtgtag cgaacaccct tgccgttgta gggctctgcg ggtcgcgttt
    21301 gacggatccg agctgcgatt cggcctacta attgcttgtc gggagactcg agatgaacgc
    21361 gggggaactt cgttcccgcc tcgtcgatgg tctcgactct gacggaaacc gtcttgggga
    21421 tgtcgagaat cggctgatga gaaagcccca ccgtcatctt gagttggtcg ttgctgaact
    21481 ccgcgcgata accgacccct cggaagtcga gtgaacgctt gtacccagtc gcgacgccct
    21541 cgaccatgtt gccgagaatc gcgcgagtga ggccctggta ctgaaggccc gtcttgcccg
    21601 ccgcgcctgg ggcaggcgca actgtgagct tgccctcgtc ttgagtgacc acgacctccg
    21661 gacggaggct ttgcgtcagc tcgcccttgg ggcccttcac cgtgatgttt tggccgctaa
    21721 tcgtgacctt gacaccatcg gcaatcttaa cgggcttctt gccgtttctg gactgctttc
    21781 ctgtttgtgt ttcggtcatc accaaacctc gcacagaacc tcaccgccca cgcgcttatc
    21841 tcgcgcatcg cggtcggtca tgatgcccac ggacgtcgaa aggatcgaaa cgcccatacc
    21901 gttctgaacc cgtggaatgt cacgatggcc cacgtagaac cgtcgacccg gacgactcga
    21961 tcggcgcatg ccgacgaagg cgctctgacg gtcgcgaccg tacttgagga agatcgtgag
    22021 gctcttttga atcccatctt ctacgcggta gtcgtcgatg aagccttcct gcttgagaat
    22081 ctcggcgagc tgcgccttga gattcgacga aggaagagtc gttcgttcgt gctgggccgc
    22141 agcggcatta cggatgcgcg tcagcatgtc agcgattgga tcggtcatca tgactgcacc
    22201 tccttaccag ctcgatttcg tgagcccggg aatgtcgccc gagagcccat atttacgtag
    22261 acagatgcgg cacatctcga acttgcggta gtaaccccgc gggcgtccac agaccttgca
    22321 gcgatttcga tgtcgcaccg aaaacttcgg tgtcttttcc aacttggcaa atgcgcgctt
    22381 actagccatc tgcgtcctct agttcctgaa cggcatgccg agcgacttta gcaaggcacg
    22441 tccctgttca tcggtttctg ctgtggtaac aaaggtcacg ttcatgccct tgatacgatc
    22501 gattttgtcg tagttgattt ccgggaacac aatctgctcg cggatgccga gcgtgtagtt
    22561 gccccggccg tcgaatgctt tcgggctgat gcccttgaaa tcgcgtgtgc gcggcaaagc
    22621 gatggagatc agcctgtcga cgaagtcgta catctgctct ccgcgcagcg tcaccatcac
    22681 gccgatgggc attccctcgc ggagcttgaa tgtggcgatc gacttcttcg cgcgggtgat
    22741 gatcggcttt cgtccagtga ttgcagtcag ctcctctacg gctgactcga tcagcttcgc
    22801 attctgcacg gcttcgccga ggcccatgtt gatgacgact cgatccagct tcgggacctg
    22861 catgacgttc cggaagctga actccttcat caactgaggg atgatctcgt ctttgtattt
    22921 cttgcggagt cggggttcgt attcactcat gtcgacctca atccaggacc gagccgctct
    22981 tcacggccac gcgaacgcgc ttttgggggt ccttcttgtc ggcttcgatg cggacgcgtg
    23041 tgcgttcctc ggtcttcggg tcgaccagca tcacgttgga ggcgtgaatc ggcatctctt
    23101 tctcgacgat gccagcctct ttgaagcggc gcgggctctg gtgccgcttc acgcggttga
    23161 ccccctcgac gaggatcaag tccgctcgca ccacctgaag aacgcggccc ttctcgccac
    23221 gatccttgcc tgcgatgacc tgaaccaagt cacccttttt gatgcgcgca gtcatcacac
    23281 cacctccggt gcgagagaaa cgatcttcat gaatcgcttc gcgcgaagct cacgggctac
    23341 cggtccgaag atgcgggtgc caatgggctc gttgtctttg gtgatcagca cggcgctgtt
    23401 cgtgtcgaac ttgatgtagg tgccgtccgg ccgctggtac tcacgcttgg tgcgaacgac
    23461 gaccgcgcgg gccacttcac ccttcttcac gcgagcagtc ggaagagctt ccttcaccga
    23521 aacgacgatg acatcgccga ggccggcata gcggcggcgc gaaccaccaa gcaccttgat
    23581 gcaggcaact cgtttggcgc cactgttgtc ggctacgtcc agctgtgttt ctgtctggat
    23641 catgattata cctccggcgg cctctcgatg aggcggacca cctcccagcg cttcgttttg
    23701 gagcgcgggc ggcaagcgcg aatctcgacg acgtcgttcg tgcggtactc gttggcttcg
    23761 tcatgcgcat ggaacttctt cttgcgcttg acgtatttcc catagaacgg gtcacgggcg
    23821 cggcggatga cctccacgac cacggtttgt tgggctcgcc cctcggactg cgtctcgttc
    23881 acgacgcgtc cgaccaagcg acgacgggca ccacgctcgc gcgtggcctt cggggtcggt
    23941 tgtcctggct cagcagcagt catggccgat cactccttat tggccgcttc gacgcggcgc
    24001 tcggtgagaa tggtcttcac tcgggcaaga tcccggcgaa gcttttgaat cgagctcgtg
    24061 tcatcgagct gattggtgtg attgtcgaac cgcgccttcc aaagctggcg gcgcaaatcg
    24121 ccctcgatga ccgacagctc ttcgagcgtg ttcgcacgaa tgtcttcggg cttcatagct
    24181 cagtctccct cgaacggaac gtcgtcttga gcggaagctt gtgcgcggcg agctcgaaag
    24241 cttcacgcgc aacgtcctcc gggacgcctt gaatctcgta cagcatgcgc ccgggcttga
    24301 tgaccgcgac ccaaccctcg acgctgccct tacccttacc cattcgagtc tcgagcggct
    24361 tcttcgtgat cggcttgtcg gggaagatgc ggatgaagag ctgcccctga cgtttcacct
    24421 tgcgctgaat cgtcatacga gccgcttcga tctgtcgctg cgtaatccgg cccggctcga
    24481 gcgcctgcag gccgaagtcg ccgaacgaga tcttgccgcc tcggtaggcc ttgccgcgca
    24541 tgcggccctt catctgtttg cggaacttgg ttttctttgg ttgaagcatg gctcgtcctt
    24601 agactcgggc tccgacggcc ccggcacctg ggcgctgcgg aagctgctca cccttgaaaa
    24661 tccagcattt gatgccgacg gtgccggctt tcgtgtgcgc ggtgacctcg ccgtagtcga
    24721 tgtcggcgcg aagcgtatga agtggcacgc ggccctcgcg ataccactcg gttcgcgcaa
    24781 tctcggctcc accaaggcga cccccggcgg tcacgcggat gcccttcacg ccgaacttca
    24841 tcgccgtctg cacggccttc ttcatggctc ggcggaaagc aacacgacgc tcgagctgcg
    24901 tggcgatgtt ctccgcgacg agctgggcgt tggtctcggc cttacggacc tcttggatgt
    24961 cgagaaagag ctcgttctgg gtgagactct ggaggtcacc cttgagctgt tcgacgcccg
    25021 cgccgcgctt gccgatgatg attcccggac gcgaggtgta gatgacgacc ttggctttgt
    25081 tcgcggcgcg ctcgatctcg atatcggcga tcccagcatg cgcgaacttt ttccggattg
    25141 cgctcttgag cttcaaatct tcatgaagcc atttggcgta gttcttgtcc tcgtaccact
    25201 tggacttcca cgtcttgacg acgccgagac ggaaaccaaa aggatgtact ttctgaccca
    25261 cggttcagct ctcttccccg tcgctcacga cgacggtgat gtgactcatt cccttgacga
    25321 cgcgcgtagc gcgaccttgt gcacgcggcc gccagcgacg catgtgctga ttcggcgcca
    25381 tgtccgcgaa agccgtcttc acgaacaacg cgtcgaggtt gatctcttcg ttcttctgct
    25441 tggcgttggc gatggcgctc ccgatggcct tcgccacgat gggagccgcc gccttggtgg
    25501 tgaaacgaag ctcattgagc gcatcggcaa cgctctttcc gcgcaccatg ttgagcacga
    25561 tgcgtgcctt acgtggcgaa atccgttgaa accgtgcttt tgcgaccgct tccatgatca
    25621 tttcttccct gtctgcggat tgatcttgcg gccgcccttc ttgtccgacg cgtgaccgcc
    25681 gaacgtccgg gtgggcgcga actcaccgag cttgtgccct accatgttct cggtcacgaa
    25741 gaccgtgacg aacttccgtc cgttgtgcac ggcgaaggtg tgcccgacga aatcgggcgt
    25801 cacggtcgac cggcgcgacc aagtgcgaat catcttcttg tcgccagact tgttcgcagc
    25861 ctcgaccttg tcttccaagt gctggtcgat gaacggaccc ttttttactg aacgcgccat
    25921 tacttcttct tccgcccgcg aacgatgtac ttgttcgtcg acttgttctt tctcgtcttc
    25981 aggcccttgg cgagctgacc ccacggcgag cagggatgac gtccgcctga cgtgcgacct
    26041 tcaccaccac ccatcgggtg gtcgaccggg ttcattgtgg tgccgcggtt gtgcggccgg
    26101 cgaccgagcc aacggcttcg gccagccttg cctagagtca ggcgggcatg ctgagagttg
    26161 ctcacctgcc cgaccgatgc gcgacagttg aggtgcacca tccgaacctc gccggatgga
    26221 aggcgaacct gagcgtagtc cccgtccttg gccatgagct gagccgcggt gcccgccgag
    26281 cgcacgagct gcgcgccctt gccgatcttg agctcgatgt tgtgaatcat cgtgccgagc
    26341 ggcatgtgtc ggagcggcat ggcgttgccg ggcttcacgt cggcatgacg tgaggagagc
    26401 acttgctgac ccacctgcag accgtcggga gcgagaatgt aagccttttc cccatcggcg
    26461 tagtgcacga gcgcgatgcg cgccgagcgg ttcggatcgt actcgatggc attgacggtg
    26521 gctggaacgc cgatcttgtt tctcttgaaa tcgaccctgc ggtagcgctg tttgtgtccg
    26581 ccaccacgga accgcgaagt gatgcgcccc tggctattgc ggcccgcagt ctgcttcttg
    26641 gactcgagga gcttcttttc cggcttcccg cgggtgatca tgtcgaaatc gggcgcttca
    26701 tagaagcggc gcgacggcga cgtgggtttg aatttacgga ttgccatctt tagacaccct
    26761 cgaagaagtc aatcgtctcg ccctctcgaa gcgtgacgat ggcctttttc cagttttggg
    26821 ttttcgcgta gcctttgccc atgcggcgca tgtgaccacg catgaccagc gtgcggacgt
    26881 cgcgaaccgt gactttgaac aaagtttcga cggcgtcctt gacctggagc ttgttggcgc
    26941 cgaggtcgac ctcgaacgtg tacttgttct cctcttcgcg caacttggcg cccttctcgg
    27001 tgagatacag cgggcgcttg atgacttgct ctacgtgcat cactgacacc ttgcttccag
    27061 ggcttgcacc gcggtctgcg tgagtacgag atgctcgtga cgaagcaaat cgtagaggtt
    27121 cacgccctcg ggtggcagaa ccgaatggcc ctgcaggtta cgcgcgctga gacgcagtct
    27181 ttcgttctct ttggcgtcga cgatgagagc gccgccattg acctcgagct tatcgaggac
    27241 ctcggcgagc ttcttcgtct tgatttcgga gagctcgaag tcctgcacga cgatcagctg
    27301 accttcgcga agcttgaggc tgagcgcgcc cttgagcgct ccgagccgca tcttccgagg
    27361 aggcctgtaa ctgaagtcgc ggggctttgg accatggacc gtgccgccac cgacgaaaat
    27421 cggagcgcgt ctggatccat gacgtgcgtt gccggtgccc ttctgcttgt agatcttgcg
    27481 cttgctgagg ctgacctcgg cccgggtctg ggacgaagcc gcgcccttgc gctggctcgc
    27541 gagctgggct ttgagaacat cgtagaggag gctctcgctg acggttgcat cgaagacgct
    27601 gtccgagagt tccatctctc cgacgtcctt cttactcaag ttgtagacct taaccttggg
    27661 catggaccta ccgccttacg acttgatttc cacgtcgaca cccgctgaaa gatccagctt
    27721 catgagggcg tcgagtgtct gttgcgtggg atccaggatg tccaagagac gcttgtgagt
    27781 tcgaatctcg aactgctccc gcgacttctt gtccacgtgg ggcccacgta acacggtgta
    27841 cttgtttatg cgtgtaggca gcggaatggg acccgcaacc cgcgcaccag tgcgcttcgc
    27901 cgtatcgacg atctccgtgg ccgattggtc tagcaatcgg tgatcgtaag ccttgagtcg
    27961 aatccggatc ttgttggctg ccattgttct actcgatgat cttggaaatg acaccggcac
    28021 cgatggttcg gccgccttcg cgaatcgcga agcgctgctt ctcttccatg gctgctggcg
    28081 tgatgaggtt gaccttcatc ttcacgttgt cgcccggcat gaccatcttc acgtcttcgg
    28141 gaagctcg
//
LOCUS       DASRZF010000108         2039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4203256, whole genome shotgun sequence.
ACCESSION   DASRZF010000108 DASRZF010000000
VERSION     DASRZF010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4203256"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1094)
                     /locus_tag="VFG22_05145"
     CDS             complement(<1..1094)
                     /locus_tag="VFG22_05145"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6415659.1"
                     /translation="MNFDHSPKVKKLQAQLTAFMEEHIYPNERVVQDFADNAESRWTV
                     PPVVEALKAKAQSEGLWNLFLPESELGAGLSNLEYAPLCEIMGRSAIAPEIFNCSFPD
                     TGNMEVLVRYGTPEQQERWLKPLLRGEIRSAFAMTEPDVASSDATNISSTIRREGNEY
                     VVNGRKWYISGAGHPNCEVMVFLGKSNPEAAGHQQHSMILIPKETPGVKLGRPMLLLG
                     YDAAPAGQHEVSFDDVRVPADNVILGEGRGFEIAQGRLGPGRIHHCMRAIGMAERAIE
                     LMVARAVSREAFHRRIADQGMVRRYIAESRIEVNQARLLTLKAAYMMDTVGNKGARSE
                     IAQIKVAAPNMAMKVIDRAIQVHGAAGVSQ"
     gene            complement(1091..1450)
                     /locus_tag="VFG22_05150"
     CDS             complement(1091..1450)
                     /locus_tag="VFG22_05150"
                     /inference="COORDINATES: protein motif:HMM:NF040046.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6285 domain-containing protein"
                     /protein_id="HET6415660.1"
                     /translation="MRSARPNAFELLEAVQEFLLKRAVPKLSGHVAFEARVAVNLLDI
                     ARREVEEGPTLFEADVAELAQLLESDASADALEAELLTLVREGDISDRWDEVITYLRH
                     RAERGLQIANPKYLEES"
     gene            complement(1447..>2039)
                     /locus_tag="VFG22_05155"
     CDS             complement(1447..>2039)
                     /locus_tag="VFG22_05155"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET6415661.1"
                     /translation="GAVAARIHSFGPEELPTLPTLDARGQLGLIRRMLDLGGQTRPIL
                     ELSLRWLRDRVPPLRSGLKLVHGDFRNGNFLVAPDGFRAVLDWEYAHLGDPMEDLGFL
                     CMKPWRFGNHEHDVGGFGSRQELFRAYEQAGGEPVDEEVVRFWEVLGTLKWGSLCTIR
                     AMLHLSRMQHSVEAAAIGRRVAETEYDLIDLIGEHP"
BASE COUNT          361 a          658 c          613 g          407 t
ORIGIN      
        1 tcttgtgaga caccggcggc gccgtgcact tggatcgccc tgtcgatgac cttcatcgcc
       61 atgtttggag ccgcgacttt gatttgcgca atctccgaac gcgctccttt gtttccaacc
      121 gtgtccatca tgtacgcggc tttgagcgtc agcagcctcg cttggttgac ctcgatccgc
      181 gactccgcga tgtaacgccg gaccatgcct tgatcggcaa tccgccggtg gaaagcctcg
      241 cggctcacgg ctcgagcgac catcagctcg atcgcccgct cggccatccc gatggcgcgc
      301 atgcaatggt gaatgcgacc gggcccgagc ctgccctggg cgatctcgaa cccccgccct
      361 tccccgagaa tcacattgtc ggccgggaca cgaacgtcgt cgaaggagac ctcatgctgt
      421 ccagccggtg cagcgtcgta accgagaagg agcatggggc gtccgagttt gacgcccggc
      481 gtttccttcg ggatcaggat catcgagtgc tgctgatgtc cggccgcctc ggggttcgac
      541 ttcccgagaa agaccatgac ttcgcagttg ggatggccgg cgccggagat gtaccacttc
      601 ctcccgttga cgacgtactc attgccttcg cggcggatcg tggacgagat gttggtcgcg
      661 tcggatgagg cgacgtccgg ttcggtcatc gcaaatgcgg agcggatctc tcctcgaagc
      721 agcggcttga gccagcgctc ttgctgctcg ggggtcccgt atcgcacgag gacttccatg
      781 tttccggtgt cggggaagct acagttgaaa atctcgggcg cgatggccga gcgccccatg
      841 atctcgcaga gcggcgcata ctccaggttg ctcagcccgg ccccgagctc ggattccggg
      901 aggaagaggt tccagagccc ttcactctgc gccttggcct tcagcgcctc gacgacagga
      961 ggtaccgtcc agcgcgattc ggcgttgtcg gcgaagtcct gcaccactcg ctcgttcgga
     1021 tagatgtgtt cctccatgaa ggcggttagc tgggcctgca gcttcttcac ttttggcgag
     1081 tggtcgaagt tcatgactcc tccagatact tcgggttcgc gatctgcagc ccccgctctg
     1141 ctcggtgccg caggtaggtg atgacctcgt cccatcggtc gctgatgtcg ccctctcgga
     1201 cgagggtcag caactctgct tccagggcat cggcgctcgc gtcgctctcg agaagctggg
     1261 cgagctcagc cacgtccgcc tcgaagagtg tcgggccttc ctcgacctcc ctccgagcga
     1321 tgtccagcag attcacagcg acacgggcct cgaatgcgac atgtccgctc agcttcggga
     1381 ccgctcgttt taggagaaac tcttggacgg cttcgagaag ctcgaaggcg tttggccggg
     1441 cagacctcac gggtgttctc ctatcagatc aatcagatcg tattccgtct cggcgacacg
     1501 ccggccaatg gccgccgcct ccaccgagtg ttgcattcgg ctcaggtgaa gcatcgcgcg
     1561 gatggtacac aacgagcccc acttcaaggt tccaagcact tcccagaaac gaacgacttc
     1621 ctcgtcgacc ggctcgccac cagcctgctc gtacgctcga aacagctcct gtcgcgagcc
     1681 aaagccgcct acgtcgtgtt cgtgattgcc gaaccgccat ggcttcatac agagaaagcc
     1741 gagatcctcc atcggatctc cgaggtgcgc gtactcccaa tcgagcacgg cgcgaaaccc
     1801 atccggtgcg accaagaagt tcccgttgcg aaagtcgccg tgaacgagct tgagaccgga
     1861 tcgtaacggc ggtacgcgat ccctcagcca tcgaagcgac agctcgagaa tggggcgcgt
     1921 ctggcctccc aggtcgagca tgcgccgaat gaggccaagc tgccctcggg catcgagggt
     1981 ggggagcgtt gggagctctt cgggaccgaa gctgtgtatg cgcgccgcaa cagccccca
//
LOCUS       DASRZF010000109         6218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2825872, whole genome shotgun sequence.
ACCESSION   DASRZF010000109 DASRZF010000000
VERSION     DASRZF010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 6218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6218
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2825872"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="VFG22_05160"
     CDS             complement(<1..903)
                     /locus_tag="VFG22_05160"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET6415662.1"
                     /translation="MSSSRSRNLREKLQTALRKERLREALGHYETLELLEPNEPRWPH
                     RKGDLLKRLGRPEPAVISYERAVVLYAQHGFVARAAAMAKVVIGIDPERRDILERVDP
                     NAARQLHRSARSKLTRTANEITQTKRLSVDAEPLVDHGPSTGDTLRFSTPPTTRHLTL
                     ELVISDAELRDRPEPSGEDASEAPSAEYLAQLPSMPLFAEVPEAVLRSIVSESSLVDL
                     EPGQNLIDRGTTADALFALVEGSVQVIRAADVDAIVLSEGDVVGISCLLDRVCYEGDA
                     TARTKVRALRISKLLLDRLVAEHPE"
     gene            993..2429
                     /locus_tag="VFG22_05165"
     CDS             993..2429
                     /locus_tag="VFG22_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HET6415663.1"
                     /translation="MSLFIRLVRAQLIFGVIFADYLAQWALYKVFRRWRRDPSTGHEE
                     AIVPAWLRRRQVRLDERNSKRMLAGMLKLRGVYIKLGQVLSIMGAFLPAVYIKRLETL
                     QDAVPPHPFDEIEKAFIGSLGARPEAFFRSMDREPVAAASLGQVHVAYMDDGLKVAVK
                     VLYPGIRDVVRIDMRVIKLMVRVYKWFVPVQNIEAVHSSLEDLLRRETDYLHEADCMR
                     RMSANFAGDDHILFPEVIDQVTTADVLTMTFMEGIKITRLDEIRNRGVDLHKLAERLV
                     KSFYKQVFVDRFFHADPHPGNFLVQFVEGSDDAKLVVLDFGAISEVGDTTVFGMVDVL
                     RGFFEQNDALVLQGIEAIGFVAEGGDRALLEQTVKVYFQKLLKLEARTPGALMNANAK
                     ELEALADPEVERRELRNLMRSFHYPDGWFFVERAAVMMFWLAGQIAPDLDQFQVGFPY
                     VLPLLSQRLGATPPSPPPDNGLQPSAGG"
     gene            2431..2877
                     /gene="ruvX"
                     /locus_tag="VFG22_05170"
     CDS             2431..2877
                     /gene="ruvX"
                     /locus_tag="VFG22_05170"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HET6415664.1"
                     /translation="MGRIVGIDPGEARIGLAISDEEASIAFPRETILARGGTAEAVNR
                     IRQALSDEEISLAVVGLPLRLDGSEGEAARRARAFGEALAASLEVEVVFWDERLTTVA
                     AERSLREMGRRGSRQREVVDQSAATILLQGYLDANKSEWWKDPDGQ"
     gene            2892..3965
                     /gene="mltG"
                     /locus_tag="VFG22_05175"
     CDS             2892..3965
                     /gene="mltG"
                     /locus_tag="VFG22_05175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HET6415665.1"
                     /translation="MGRTIARLLSLVTLLAVLGLFSVIGWLFVIYPDTSGPAAEAQEV
                     IIEVSEGMTLQKLATELKTQGVIKDAEVWTLYMRMRGLDRHLRQGKVRFPVPMTPEEV
                     ARHATTRLGRIQVRILIPEGFSRFEIANRLEEYGVCSAEDFLAATEDVSLLASYGVEA
                     HDAEGYLFPDTYEFDDQTKPRRVAERMLTNWSRKYEEIQSEYAQQLSRMKSWTTHDIM
                     TLASIVEKEAAVPSERPRIAGVFWNRLTSKRFLPRRRLQADPTVQYGCVAVPDAAPSC
                     EGFDGSITRAMLDDPENPYNTYRHSGLPPGPISNPGLASILAVIDPEVHDFYYFVASG
                     SGRHDFSKTLRQHNVAVRRNGQK"
     gene            3962..4975
                     /locus_tag="VFG22_05180"
     CDS             3962..4975
                     /locus_tag="VFG22_05180"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HET6415666.1"
                     /translation="MSPRRSQKGQLFGKEDSQLGLFDRPDPAAPDPLASAQLELESLG
                     ARLPAKLRFGTSSWTFPGWAGLVYHKRYPNQRTFLRDSLREYAEHPLMRTVGIDRGYY
                     SPVPEEDLAHYASQLPDDFRAAMKVWQGVTMPGFPRHPRYGANAGKQNPEFLDVEVFA
                     RAVHEPARVAFARNMGPWILEIAPAPSPLDPAWFCERLDAFLEDAPRDFPFAVELRDR
                     RLLTDQYVRTLRKHGASHVFNFWSRMPSLGRQMEVTGLLESSMIVARLLLPPGGRYAE
                     LKDAYAPFDRLVAPQHEMRQDVVRLVRAALDKDKECYVIVNNKAEGSSPLTVRSLAEL
                     VAG"
     gene            5012..5626
                     /gene="cysC"
                     /locus_tag="VFG22_05185"
     CDS             5012..5626
                     /gene="cysC"
                     /locus_tag="VFG22_05185"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF003013.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HET6415667.1"
                     /translation="MSEPKAENLTWHIGEVDRDARATAHGHRGAILWLTGLSGSGKST
                     IGHRVERMLIERGAFAYVLDGDNIRHGLNRDLGFSPEDRVENIRRIGEVARLFADAGA
                     LVLSAFISPYRQDRDRVRALMGPGEFIEVFVDTPLEICEARDPKGLYKKARAGEISNF
                     TGLDAPYEAPENPEVHLETADLSVDGAASQVIRYLEQQQILGAR"
     gene            5626..>6218
                     /locus_tag="VFG22_05190"
     CDS             5626..>6218
                     /locus_tag="VFG22_05190"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6415668.1"
                     /translation="MPTREEMWGKVPEEADVVVIGGGITGAGIARDAARRGLSVVLFE
                     QNDIAYGTSSRSSKLIHGGLRYLESYEFSLVFESVSERRVVMDLAPHLVNPLAFLFPV
                     YQGARKSLRMISAGMWLYDGLALFRSPKRHRTLKPVEVAKEEPLLKQEGLQGAPVYYD
                     CSTDDARLTLETVIDAIRNGGVAVNWVRVDALTKNEQG"
BASE COUNT         1153 a         1903 c         1947 g         1215 t
ORIGIN      
        1 ctcggggtgc tctgcgacga gccgatcgag caagagcttg ctgattcgga gggcgcgcac
       61 cttcgtacga gcggtcgcgt cgccttcgta acaaacccga tcgagcaggc aggagatgcc
      121 gaccacgtcg ccttccgata ggacgatggc gtcgacatcc gcagcgcgga tcacctgaac
      181 gcttccctct acgagcgcaa aaagggcgtc ggccgtggtg cccctatcga tgaggttctg
      241 accgggttcg aggtcgacca ggctcgactc gctgacgatg ctcctgagaa ccgcctcggg
      301 aacttccgcg aacaggggca tcgacggaag ctgcgcaagg tactcggcgg agggggcttc
      361 gctcgcatct tcgcccgagg gctcgggccg atctctgagc tctgcgtccg aaatgacgag
      421 ctcgagcgtc aggtgtcgcg tcgtcggggg ggtcgagaac ctcagcgtgt cccctgtcga
      481 gggtccgtgg tcgaccaggg gctccgcgtc gactgagagg cgcttcgttt gtgtgatctc
      541 gttggcggtg cgggtcagct tgctccgggc cgagcggtga agctgtcgcg ccgcattggg
      601 gtccacgcgc tcgaggatgt ctctgcgctc tggatcgatc ccgatcacca ccttcgccat
      661 cgccgcagcc cgagcgacga atccatgctg tgcgtagagc acgaccgccc tctcgtatga
      721 aatcaccgcc ggctccggcc gtcctagacg cttcaagagg tcacctttgc ggtgcggcca
      781 gcgtggttcg ttcggctcga gcaactccaa ggtctcgtag tgaccgaggg cttccctcag
      841 acgctccttg cggagcgccg tttggagctt ttctctcagg tttcgactgc gactactcga
      901 cacgaccccg gaaccttata cccataaccg tccaccctaa agcactggga cgtcgcccaa
      961 tcgctgctac gctcccctgg acgcccaacc ctatgagtct cttcatccgc ctcgttcgtg
     1021 ctcagctgat ttttggcgtc attttcgccg actacttggc tcaatgggcg ctctataagg
     1081 tcttccggcg ctggcgccgg gatccttcga ccgggcacga ggaagccatc gttcccgcct
     1141 ggcttcggcg acggcaggtc cgcctcgacg agcggaactc caaacggatg ctcgctggca
     1201 tgctgaagct tcgcggcgtc tacatcaagt tgggtcaggt tctgtcgatc atgggcgcgt
     1261 tcctgcctgc cgtgtacatc aaacgcctgg agaccttgca ggacgccgtg ccgccgcacc
     1321 ctttcgacga aatcgagaag gcgttcatcg ggagtcttgg agcgcggccg gaggctttct
     1381 ttcgaagcat ggatcgggag ccggtggccg ctgcttcgct gggccaggtg cacgtcgctt
     1441 acatggacga cggactcaag gttgccgtca aagtcctcta tcccggcatt cgcgatgtcg
     1501 tccgtatcga catgcgcgtg atcaagctga tggttcgcgt ctacaagtgg ttcgtgccgg
     1561 tgcagaacat cgaggccgtt cactcgtcgc tcgaagatct cctgcgtcgc gaaacggact
     1621 atttgcacga ggcggattgc atgcgccgca tgtcggcgaa ctttgcgggc gacgaccaca
     1681 tccttttccc agaggtgatc gaccaggtca cgaccgcgga tgtgctcacg atgaccttca
     1741 tggagggcat caagatcacg cgcttggacg agatccgaaa ccgtggggtc gatcttcaca
     1801 agcttgcgga gcgcctggtg aagagcttct acaagcaggt tttcgtcgat cgattctttc
     1861 acgccgatcc acatcccggt aactttctcg ttcagttcgt agagggctct gatgacgcca
     1921 agctggtcgt tctcgacttc ggggcgatca gcgaggttgg cgacacgact gtgttcggca
     1981 tggttgacgt cctacgcggt ttcttcgagc aaaacgatgc gctcgtcctg cagggcatcg
     2041 aggcgatcgg cttcgtcgct gaagggggcg atcgcgcgct gctcgagcaa acggtgaagg
     2101 tgtattttca gaagctgctc aagctcgagg cacgcacgcc cggggctctg atgaacgcaa
     2161 acgcgaaaga gctcgaagca ttggccgacc ccgaagtcga gcggcgcgag cttcgcaatc
     2221 tgatgagaag cttccactat cctgacggct ggttcttcgt cgaacgagcg gcggtcatga
     2281 tgttctggct ggccggtcaa atcgcgccgg acctcgatca gtttcaagtg ggcttcccgt
     2341 acgtcttgcc tctgctgagt cagcgcctcg gggccacccc cccttcgccg ccacccgaca
     2401 atggcctgca gccctcggcc ggaggctgac gtggggcgaa tcgtcggcat cgatccgggg
     2461 gaagcgcgca ttggtctcgc catttccgat gaagaagcgt ccatcgcctt cccgcgggag
     2521 acaatcctcg cgcgtggcgg gactgcggag gcggtcaatc ggattcggca ggccctatcc
     2581 gacgaggaaa tctctctggc cgtcgtgggg ctgccgctcc ggctcgacgg gagcgaaggt
     2641 gaggcagctc gacgcgcccg ggctttcggc gaggctctcg cagcctccct cgaagtcgag
     2701 gtggtattct gggacgagcg cctcaccact gtggctgcgg agcgctcgct ccgggagatg
     2761 ggccgaagag ggtccaggca gcgcgaagtc gtcgatcagt cggcagccac aatcttgctg
     2821 caaggctatc tagacgccaa taagagtgaa tggtggaagg acccggacgg ccaataagga
     2881 tcgagagcga tgtgggccgg acaattgccc ggctgctgag cctcgtcacg cttctggccg
     2941 ttctcggcct tttcagcgtc atcggctggc tgttcgtgat ctatcccgat accagcggtc
     3001 cggcggccga ggcccaagag gtcatcatcg aggtgtccga ggggatgacg ctgcagaagc
     3061 tcgcgacgga gctgaagacg cagggtgtca tcaaggacgc cgaagtttgg acgctgtaca
     3121 tgcgaatgcg cggtctcgat cggcacctgc ggcaaggaaa ggttcgcttt cccgtgccga
     3181 tgacgcccga ggaggtggcc cgccatgcga cgacccgact cggacgcatt caggtgcgga
     3241 tcctgatccc cgaggggttc tctagattcg agatcgcaaa ccgcctcgaa gagtacggcg
     3301 tttgctcggc cgaggatttt ctcgctgcga ccgaagatgt ctcactgctg gccagctatg
     3361 gggtcgaagc gcatgacgcc gaaggctatc tcttcccaga cacctacgag ttcgacgacc
     3421 aaaccaagcc aaggcgagtg gccgagcgca tgttgacgaa ttggagccgg aagtacgaag
     3481 aaattcaaag tgagtacgcc cagcagctga gccgcatgaa gagctggacg acgcacgaca
     3541 tcatgacttt ggcgtcgatc gtcgagaaag aggccgccgt gccgtcggag cgaccgcgga
     3601 ttgctggtgt attctggaat cgcttgacgt cgaaacgctt cctgccgcgg cgcaggcttc
     3661 aggcggatcc cacggttcag tacggatgcg tggcagtccc cgacgcggcg ccatcctgcg
     3721 agggcttcga tggcagtatc acgcgcgcga tgctcgatga tccggaaaat ccatacaaca
     3781 cataccggca ttcggggctg cctccaggcc cgatcagcaa cccgggtttg gcatcgattc
     3841 tcgcggtcat cgatcccgag gttcatgact tctactactt cgtggcgagc ggcagtggcc
     3901 gccacgattt cagcaagacg ctccgacagc acaacgtcgc agttcgccgc aacgggcaaa
     3961 aatgagcccg cgcagatcgc agaagggcca actcttcggc aaagaggatt cacagctcgg
     4021 gctattcgat cgacccgatc ccgctgcccc cgatcccctg gcgagcgcgc agctcgagct
     4081 cgaatcgctt ggggcgcggc ttcccgccaa gctccgcttt gggacgtcca gttggacttt
     4141 ccccgggtgg gccggcctcg tctatcacaa gcggtatccc aaccaacgca cgtttcttcg
     4201 agactcgctt cgcgagtacg ccgagcatcc actcatgcgc acggtgggca tcgatcgcgg
     4261 ctactacagt ccagtccccg aagaagacct agcgcactac gcgagccagt tgcccgacga
     4321 tttccgcgcc gcgatgaagg tctggcaggg tgtcacgatg cccgggtttc cgaggcatcc
     4381 gcggtacggg gcgaacgccg gaaaacaaaa cccagagttc ctcgatgtcg aggtctttgc
     4441 gcgcgcggtg cacgaacccg cacgcgtcgc ctttgcacga aacatgggtc cctggattct
     4501 cgagatcgct cccgcaccat cgccactcga tcctgcttgg ttctgcgagc ggctcgatgc
     4561 gtttctcgaa gacgcgccgc gtgatttccc gttcgcggtc gagttgcgcg atcgaaggct
     4621 tctcaccgac cagtacgtgc gcacgctgag gaagcacggt gcctcccacg tcttcaattt
     4681 ctggtcgcga atgccatctc tcggtcggca gatggaggtc acagggctgc tcgagagctc
     4741 catgatcgtc gcgcgcctac tcttgcctcc gggcggacgg tacgccgagc tcaaggacgc
     4801 gtatgcccca ttcgatcggc tcgtcgcgcc tcagcacgag atgcggcagg acgtcgtgcg
     4861 gctggttcgg gcggcgctcg acaaagacaa ggaatgctac gtaatcgtca acaacaaggc
     4921 cgaaggctcg tcgccgttga cggtgaggtc cctggccgag ctggtcgctg gctagcttgc
     4981 tcgccctccg ggcggggcta tgttgtgcgt catgagcgag cccaaggccg agaacctcac
     5041 ctggcacatc ggagaagtcg accgagacgc tcgcgcgact gcacacggac atcgcggcgc
     5101 cattctctgg ctcaccggtc tttccggctc tggtaagagc accatcgggc atcgcgtcga
     5161 gcgaatgctg atcgagcgcg gcgcctttgc gtacgttctc gacggcgaca acatccgaca
     5221 cgggctcaac cgtgatctcg gtttttcgcc cgaggataga gtcgagaaca ttcgccgcat
     5281 cggcgaggta gcgcgcttgt tcgccgacgc tggcgctttg gttctttcgg cgttcatttc
     5341 tccctaccgt caggaccggg accgtgtacg agccctgatg ggcccagggg agttcatcga
     5401 ggtgttcgtc gacacacccc tcgagatctg cgaggcacgc gatccgaagg gcctctacaa
     5461 gaaggcccgt gccggcgaga tttcgaattt taccgggctt gatgcgccgt acgaggcccc
     5521 ggagaatccg gaggtacacc tcgagactgc ggacctttcc gttgacggag ccgcatctca
     5581 ggtcatccgt tacctcgaac aacagcagat actaggagcc cgctgatgcc tactcgtgaa
     5641 gaaatgtggg gcaaggttcc cgaagaagcg gatgtcgtcg tcattggcgg tggcatcacc
     5701 ggcgccggaa tcgcgaggga cgcggcgcgg cgtgggctca gtgtcgtgct cttcgagcaa
     5761 aatgacattg catacggcac tagctcgagg tcgagcaagc tcattcacgg agggctccga
     5821 tacctcgaaa gctacgagtt cagcttggtg ttcgagtccg tgagcgagcg ccgtgtcgtg
     5881 atggacttgg cgcctcatct ggtcaatcca ctcgcattct tgttccccgt ctatcagggc
     5941 gcgcgaaaga gcctacgcat gatcagcgcc ggaatgtggc tctacgacgg tctggccttg
     6001 tttcgttcgc caaagcggca ccgcacgctg aaaccagtcg aggtcgcaaa agaagagcca
     6061 ttgctcaaac aagagggcct gcaaggtgcg ccggtttact acgactgctc cacggacgat
     6121 gcgcgactga cgctcgaaac ggtcatcgat gcaatccgga acggtggcgt tgcggtgaac
     6181 tgggtgcgag tagacgcgct gaccaagaac gagcaggg
//
LOCUS       DASRZF010000110         2337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3515791, whole genome shotgun sequence.
ACCESSION   DASRZF010000110 DASRZF010000000
VERSION     DASRZF010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3515791"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..1582)
                     /locus_tag="VFG22_05195"
     CDS             complement(176..1582)
                     /locus_tag="VFG22_05195"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET6415669.1"
                     /translation="MSSLDSALIAIERACGSGSVLRDPSLLEGYSHDESEVEPCMPAA
                     VVRASSTAEVSAVMKAAYEHEIPVIPRGGGTGRVGGAVPVPGAIVLALEKMNAVRGID
                     KGELTTRVQPGVITGELQRITEEEKLFYPPDPNSLDSCAIGGNIAANAGGPRAFKYGV
                     TREYVMAMDVVMADGTILKLGRETKKGVTGYDLASLIVGSEGTLGIVTEATLRLIPKP
                     EGTVTALACFSSIDEVAPVVSTLLAQGQLPSCIELLDEEAIRIVRPEAGLTIPEETKA
                     MLLIELDGEVAQLEGDLERTGNVLFDLDALEVLVAQHSGERERLWASRRELSHSLRRQ
                     AKNKLAEDVVVPRTKMADLLKYCAELAGQHHLVMPTYGHAGDGNLHVNFLWNEPEEKV
                     HVDAAIKSLFKRVIELRGTLSGEHGIGVLKAPYLSMEQSPELIALQEKIKDVFDPKHI
                     LNPGKIFPADAKRFHGAC"
     gene            1720..>2337
                     /locus_tag="VFG22_05200"
     CDS             1720..>2337
                     /locus_tag="VFG22_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415670.1"
                     /translation="MRFFAYVLLAAAAVVGCGETAAPTIPVDAGRDAAIDLDAGVDAA
                     VEDGGADASIPDGGILDGGLPWCETSALCPTCPAIDSLCDDAKPCAIGEVCVATGCED
                     FARCFTTGGGACQGDEDCANPAYTCDQTIGRCLRIEPGCEDSSDCLAGFACESEICVD
                     RRVPCEFGSDCPHGFTCFFPSPDQRFCRRISRPCDDDLDCLTLGVP"
BASE COUNT          390 a          747 c          728 g          472 t
ORIGIN      
        1 gttctctaaa acccagtggt ggaacgccca ctcgggccaa ggtgcgacgg gatctacgct
       61 tgcgtctggg ccggcatccg cacccccgtc gggcgtgatg ctcgttgacg acgagcaggc
      121 tacgagggta gaaagtacga aggagccaat aagtccaagc atgcgccaaa gctcgctagc
      181 aagcgccgtg aaagcgtttc gcgtcggcag ggaagatctt gccggggttg agaatgtgct
      241 tcggatcgaa gacatccttg atcttttcct gcagagcgat cagctccgga gactgctcca
      301 tcgagaggta aggggctttt agaacgccga tgccgtgctc gccgctcaag gtgcctcgaa
      361 gctcgatcac ccgcttgaat agggacttga tcgccgcatc gacgtgcact ttttcctcgg
      421 gttcgttcca gaggaaattc acgtgcaggt ttccatcacc cgcgtgccca taggtcggca
      481 tgacgaggtg gtgctggcct gcgagctctg cgcagtattt gagcaagtcg gccatcttcg
      541 tccttggcac gacgacgtcc tcggcgagct tgttcttggc ttgtcgcctc aagctatgac
      601 tgagctcgcg cctcgaggcc cagaggcgtt cacgctcacc gctgtgctgc gctactagaa
      661 cctcgagcgc gtccaagtcg aaaaggacgt tgcccgttcg ttcgagatcg ccttcgagct
      721 gtgccacttc gccatcgagc tcgatcaaga gcatcgcctt ggtctcctcg gggatcgtca
      781 agccggcttc aggacgtacg atgcggatcg cttcctcgtc gagcaattcg atacaagaag
      841 ggagctggcc ttgggcgagc agggtcgata cgaccggcgc gacctcgtcg atgctcgaga
      901 agcaggccag cgcggtcacc gtcccctccg gcttcgggat caaacggagc gtggcttcgg
      961 tcacgatccc gagcgtccct tcgcttccga ctatcaagga tgcgaggtcg tagcccgtga
     1021 cgcccttctt ggtttcgcga cccagcttca ggatcgttcc gtcggccatc acgacgtcca
     1081 tcgccatgac gtactctcgg gtcacgccat acttgaacgc gcgcggtccg cccgcgttgg
     1141 ctgctatgtt gccgccgatc gcacaggagt cgagcgagtt tggatccgga gggtagaaga
     1201 gcttctcttc ttcggtaatt cgctggagct cgcccgtgat gacaccgggc tgcacgcgcg
     1261 tggtgagctc tcccttgtcg atgcctcgga ccgcgttcat cttttcgagc gccaaaacga
     1321 tcgcaccggg caccggcacg gcgccaccga cacggccggt cccaccgcct cgcggaatca
     1381 ccggaatctc gtgctcgtag gccgctttca tgaccgccga tacctccgcg gtgctcgaag
     1441 ctcggacgac cgccgcgggc atgcaaggct cgacctcgct ctcgtcatgc gagtatcctt
     1501 cgagcaagct cgggtcacgg aggaccgagc cgctcccaca tgcgcgctct atcgcgatca
     1561 aggcgctatc gagtgacgac atgttctcag ccttgggacc ggagacccga gcggtcaagg
     1621 ccccagagcg cccggggtgc gagcgcgggc cggaggcgct aagctccgat gccccgaccg
     1681 accaggcgga cctcgcaggt ctctcggacc ctcttgacga tgcgtttctt tgcttacgtt
     1741 ctgctggccg ctgcggcggt ggtcggctgt ggtgaaaccg ctgcacccac gatcccggtg
     1801 gacgccgggc gcgacgcggc catcgatctg gacgccgggg tcgacgccgc tgtagaggac
     1861 ggcggcgccg acgcgagcat ccccgacggc ggcattctgg acggcgggtt gccctggtgc
     1921 gagacgagcg ccctctgccc aacttgcccg gctatcgatt cgctttgcga cgacgcgaaa
     1981 ccctgcgcca ttggcgaggt ttgtgttgcg accggctgcg aggattttgc gcgttgcttc
     2041 accactggtg gaggcgcttg ccagggcgac gaggactgcg ccaatcccgc gtacacctgc
     2101 gatcagacca tcggtcgttg ccttcgcatc gagcctggtt gcgaggactc gagcgattgc
     2161 ctggcgggct tcgcatgcga gagcgagatc tgcgtcgatc gcagggtccc ttgcgagttt
     2221 ggctccgact gtccgcacgg gttcacatgt ttctttccga gtcccgacca gcgattctgc
     2281 cggcgcatca gtcgtccctg tgacgatgat ctcgactgtc tcaccctcgg cgttcct
//
LOCUS       DASRZF010000111         6556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_765645, whole genome shotgun sequence.
ACCESSION   DASRZF010000111 DASRZF010000000
VERSION     DASRZF010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 6556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6556
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765645"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="VFG22_05205"
     CDS             <1..473
                     /locus_tag="VFG22_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415671.1"
                     /translation="PPRAVSSISGEGLAGLSEAMVLALPEESRLEAARALTRAKEARL
                     RIGSEIVQSCTAVSVTVGLTPIPFSDMVVLGPLQAMMVSSLAYLSGRSWGKKTVAEWL
                     GSLGVVGGLGMGLRFSAQTIAKFVPGAGNVVSAGVAGAGTTAMGQSAIKYFLKD"
     gene            complement(463..1131)
                     /gene="lipB"
                     /locus_tag="VFG22_05210"
     CDS             complement(463..1131)
                     /gene="lipB"
                     /locus_tag="VFG22_05210"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HET6415672.1"
                     /translation="MLFCRLGTVDYAEAHRAQKELQSKRIAGEIGDTVLLLEHPPVLT
                     MGRSAKVQHVLASESELEALGVELHEVGRGGDVTYHGPGQLVAYPIVDLKPDRKDVRK
                     YVSSLEETMIRTCADFGLTANRVAGLNGAWIGERKVGAVGVRISRWVTMHGLALNVNS
                     RLEHFEWIVPCGIQDKGVTSISAELGREVSVDTALELLAAHFAAQYDAEVEWQDAMPG
                     GVSL"
     gene            complement(1137..2627)
                     /gene="lpdA"
                     /locus_tag="VFG22_05215"
     CDS             complement(1137..2627)
                     /gene="lpdA"
                     /locus_tag="VFG22_05215"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HET6415673.1"
                     /translation="MEITHDAGEYITDVGTGTGTEGDADWLAMGNDYEAIVIGGGPGG
                     YVAAIRLGQLGVKTLCVEKEAMGGVCLNWGCIPSKALIAAANLANKVRNAGHMGITVK
                     DLEVDVAKMQVWKEGIVKKLTSGVSSLVKGSGGTIVMGTATLTGPSSVEVMSAEGKKE
                     TYTASKGIIVATGTQMIRIPAFEPDGDVVITAREAVSLQSAPESMVLIGGGVIGLELG
                     MVYQKLGTKITVVELMDQLLPGTDLDLVRVVQKHLKKDGADIHLKTKAVKLDKSGGRA
                     KVTIDTEGKEQVVEAEKVLVAVGFKPNSAGLGLEGVGVKLDERGHILVDEQMRTNVPT
                     VFAIGDVAGMPYLAHKASKEGEIAAEVIAGHKSARDYRGMPAAIFTDPEIATVGMSES
                     EAKAQGKKVKVGKFPFAASGRAMAVSETDGFIKVIIDEGDHQLLGVAIVGPEASDLIS
                     ESALALEMCAFAEDVALTVHPHPTLGEGVMEAFKHALGEAIHIMNR"
     gene            2613..3053
                     /locus_tag="VFG22_05220"
     CDS             2613..3053
                     /locus_tag="VFG22_05220"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6415674.1"
                     /translation="MRYLHTMIRVSDLDASLDFFCNKLGLFEHRRRDSEAGRYTLVFL
                     AAPGDRNRAANEASPLLELTYNWDPEEYTGGRNFGHLAFETENIYTLCQALMDAGVTI
                     HRPPRDGHMAFVRSPDGISVELLQKGEPLEVKEPWASMQNTGRW"
     gene            complement(3069..4070)
                     /locus_tag="VFG22_05225"
     CDS             complement(3069..4070)
                     /locus_tag="VFG22_05225"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET6415675.1"
                     /translation="MVQGSALSGYRELVAELGGQAEPLLARFRITRAALEEPQGFVSY
                     GSLIRLLEASAAELDCKDFGLRLSTRQDIGILGPLGVAVRNCETLGEAMQCASRYMFV
                     HSPAISFTPQAAEQSDRRLLVFQILLDKIGPATQVTELSIGLAARIIEMLTQKTQSLL
                     GVRFSHPSCAPIASYRAHFASPVSFSADVSAIEIDARDLDLAIHDASLQLRKLAEDYL
                     DLRHADPRTPLSIRARLVIQRSLGTGSASCKDVSAAFAVHPRTLQRQLKQEGTTFERL
                     KDEARAELARGYIANRDLPLSHVAALLDYSEQSALTRSCRRWFGRTPRGLRAELSND"
     gene            4191..6059
                     /locus_tag="VFG22_05230"
     CDS             4191..6059
                     /locus_tag="VFG22_05230"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET6415676.1"
                     /translation="MQEQDIIIKNGRVFDGKGSPATTKHLLISKGCVEKISDEEIPAR
                     DDIKVIDADGRWVMPGFIDIHTHYDAEVEAIPSLHESLRHGVTTVFMGSCSLSTAVGD
                     PTDIADMFTRVEAIPYRHLLPLMQEGKTWETHAEYVEHLNSLPLGPNVASFVGYSAVR
                     AKAMGLERALDAEAKPTEAELQTMEEVIEEGYDAGFLGLSLNGLYWDKMGGDRFRSKC
                     LPSTYASWKELRRMVRGVRERGLNLQAIPNVSTKYQILIYAAFSAGLFVRNALKTSIV
                     SIMDLRSNRLIWRALQGLGWAVNKVFRGKFRYQFLPVAFDLWADGFENVVFEEFGAGA
                     AGLHLETMAERRKLFQDPSYRRKFNRQWNNPLIPKVFHRNFGKATVMDCPDSSLIGKT
                     FREIGVLQGKSEVDAFLDLLIAYGNELRWYTVTGNDRPKPLAKMMNSESNLLGFSDAG
                     AHLRNMAFYNFPLRMLKFVKDAEDRGEPVMPLEKAVHKLTGELGEWFQIEAGTLEEGK
                     RADLVIVDPAQLDYRVEEIHEEEIPELGNYVRLVRRNPDAVPAVIVNGTLVAETGEVL
                     PEIGRTVGAGQFLRASSAGRSSQTKRMPKDRSASERPNQEQQHIPSDRAAFTSAGP"
     gene            complement(6040..6438)
                     /locus_tag="VFG22_05235"
     CDS             complement(6040..6438)
                     /locus_tag="VFG22_05235"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET6415677.1"
                     /translation="MPRVEQDFARAFASDWIDAWNRRDLDAVLAHYGEDFEFSSPVII
                     DVAGEPSGTLRGKPAMREYWTKALARLPDLHFDLDEVLTGIDTITLLYRGPRGASAET
                     FHFDVDGKVDRALACYAPARQKVSPTVPRK"
     gene            complement(6450..>6556)
                     /locus_tag="VFG22_05240"
     CDS             complement(6450..>6556)
                     /locus_tag="VFG22_05240"
                     /inference="COORDINATES: protein motif:HMM:NF014820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415678.1"
                     /translation="TALNELKRRGGKYAVVTMCIGGGMGAAGLFEAID"
BASE COUNT         1213 a         2117 c         1996 g         1230 t
ORIGIN      
        1 acccaccgcg cgccgtatcc tcgatcagcg gtgaaggcct tgcaggtctg tcggaggcca
       61 tggtcttggc gctgcccgaa gagagcagac tcgaagcagc aagggcgctg acccgcgcca
      121 aggaggctcg cctgcggatc ggtagtgaga tcgtccaatc gtgtacggcg gtctcggtga
      181 cggtcgggct cacaccgatt ccgttctcgg acatggtcgt cctcggccct cttcaagcga
      241 tgatggtgag ctccttggcc tacttgagcg gccgcagctg ggggaagaag accgtagccg
      301 agtggcttgg aagcctcggg gtggtcgggg gcctcgggat gggcctccga ttctccgcac
      361 aaacgatcgc gaagttcgtt ccgggtgctg gcaacgtcgt ttcagctggt gtcgcgggcg
      421 cggggaccac cgccatgggt cagagcgcga tcaagtactt cctcaaagac tgaccccgcc
      481 gggcatcgcg tcctgccatt cgacttcggc gtcgtattgc gctgcgaagt gagccgccag
      541 cagctcgagc gccgtgtcta ccgacacctc gcggccgagc tcggcagaga tcgaagtcac
      601 gcctttgtct tggataccgc agggaacgat ccattcgaaa tgctcgagcc tgctgttcac
      661 gttgagggca aggccgtgca tcgtgaccca gcggctgatg cgcacaccga cggcgcccac
      721 cttgcgttcg ccgatccatg ctccgttcaa ccccgctacc cggttcgccg tgagcccgaa
      781 gtccgcgcag gtgcggatca tggtttcctc gagtgagctg acgtacttgc gtacgtcttt
      841 gcgatcgggc ttgaggtcga cgatcgggta cgccaccagc tggcctgggc cgtgataggt
      901 cacgtcgccg ccgcgtccga cttcgtgcag ctccacaccc agggcctcga gctccgattc
      961 cgacgcaagg acatgctgca ccttggccga gcgccccatg gtcaagacgg gcgggtgctc
     1021 gagaagcaag accgtgtcgc caatctcgcc ggcgatccgt ttgctctgca gctccttctg
     1081 ggcgcggtgc gcttcagcgt agtctacggt tccgaggcga cagaagagca cggcgcctag
     1141 cggttcatga tgtgaatggc ttcgcccaag gcgtgcttga aggcttccat cacgccttct
     1201 ccgagagtag gatgggggtg cacggtcagc gcgacgtcct cggcgaacgc gcacatttcg
     1261 agcgcaagcg ccgactcgct gatcaggtcg ctcgcctcgg ggccgacgat cgcaacgccc
     1321 agcagttgat ggtcgccctc gtcgatgatc accttgatga acccatcggt ctcactcacc
     1381 gccatcgctc ggcccgaagc tgcgaacgga aacttcccga ccttgacctt tttcccctgc
     1441 gccttcgctt cgctttcact catgccgacg gtcgcgatct ccggatccgt gaaaatcgct
     1501 gcgggcattc cgcgatagtc gcgcgcggac ttgtgtcccg cgatgacttc tgcggcgatt
     1561 tcgccctcct tcgaggcttt gtgtgccagg tatggcatgc cggcaacgtc gccgattgcg
     1621 aagaccgtcg gcacgttggt gcgcatctgc tcgtcgacga ggatgtgtcc gcgctcgtcg
     1681 agcttcacgc ccacgccctc gagtccgagg cctgccgagt tgggcttgaa gccgaccgca
     1741 acgagcacct tttcggcttc gacgacctgt tccttcccct cggtgtcgat ggtgactttc
     1801 gcgcggccgc cgctcttgtc gagcttgacg gccttggtct tgaggtgaat gtccgcgcca
     1861 tctttcttga gatgtttctg aaccacgcga accaaatcga gatccgtgcc tggaagcaac
     1921 tggtccatca gctcgacgac cgtgatcttc gttccgagct tctggtacac catgccgagc
     1981 tcgaggccga tgacgccgcc gccgatcagc accatgctct cgggcgcgct ttgaaggctc
     2041 accgcctcgc gtgcggtgat cacgacgtct ccatcgggtt cgaacgccgg gatccgaatc
     2101 atctgcgtgc cggtggcgac gatgatgccc ttgctcgcgg tgtacgtttc cttcttgcct
     2161 tccgcgctca tcacctcgac ggagctaggt cctgtcaggg tggccgtgcc catgacgatg
     2221 gtcccgccac tgcctttgac gaggctgctg acaccgctgg tgagcttctt gacgatgccc
     2281 tctttccaga cctgcatctt ggcgacgtcg acctccaagt ctttcaccgt gatacccatg
     2341 tggccggcgt tgcgaacttt gttggcgagg ttggccgcgg cgatcaaggc cttcgaagga
     2401 atacaccccc agttgaggca gaccccaccc atcgcctctt tctcgacgca gagcgtcttc
     2461 acgcccagct gtccaaggcg aatagcagcg acatagcccc ccgggccgcc gccaattaca
     2521 attgcttcat agtcgttgcc catcgcgagc cagtctgcat caccctcagt accagtgcca
     2581 gtgccaacgt cggtgatata ctcgccggca tcatgcgtta tctccacacg atgatccgcg
     2641 tgagcgactt ggacgcgtcg ctcgacttct tttgcaacaa gctcgggctc ttcgagcacc
     2701 ggcgtcgcga cagcgaggca ggacgctaca cgctggtttt cctagcggcg cccggtgacc
     2761 gcaaccgtgc cgcgaatgag gcgtcaccct tgctcgagct cacctacaac tgggaccccg
     2821 aggaatacac cgggggccgc aacttcggcc acctggcctt cgagaccgag aacatctaca
     2881 cgctctgcca agcactgatg gatgccggcg tcaccatcca ccgtcccccg agggacggac
     2941 acatggcgtt cgttcgctcc cccgatggaa tctccgtcga gcttctgcag aaaggtgagc
     3001 cgctcgaagt aaaagaaccc tgggcatcca tgcagaacac cggccgctgg taggaccgcg
     3061 caggcgcatc agtcgttcga cagctcggcc cgcaggcccc tcggcgtccg tccaaaccag
     3121 cgccgacagc tcctggtgag cgccgactgc tcgctgtagt cgagtagagc ggcgacgtgt
     3181 gagagcggaa ggtctcggtt cgcgatgtac ccgcgcgcca gctctgcccg ggcttcgtcc
     3241 ttgagtcgct cgaacgtcgt cccttcttgc ttgagctgac gctgcaatgt gcgcgggtgg
     3301 acagcgaaag ccgcagaaac gtctttgcat gatgcggatc ccgtcccaag cgagcgctga
     3361 atcacgaggc gagcccggat cgatagcggg gtccgcggat ccgcgtgccg gagatcgagg
     3421 tagtcctccg ccagcttgcg tagctgcaag ctagcgtcgt ggatggccag gtcgagatcc
     3481 ctcgcgtcga tttcgatcgc ggacacgtcc gctgaaaacg aaaccggcga tgcgaagtgc
     3541 gcgcggtagc tcgcgatcgg agcgcaggac ggatgactga aacgaacgcc gagcaaagac
     3601 tgtgtcttct gggtgagcat ctcgatgatc cgtgccgcca agccgatgct caactcggtg
     3661 acctgcgtgg ctggaccgat cttgtcgagg aggatctgga acacgagcag acggcggtcg
     3721 ctctgctcgg cagcttgcgg cgtgaacgag atggcggggc tgtgcacgaa catgtaacgc
     3781 gaggcgcatt gcatggcttc accgagggtc tcgcagttcc tcacggcgac gccgagagga
     3841 ccgaggatgc cgatgtcttg tcgagtcgaa aggcgcaggc cgaagtcctt gcagtcgagc
     3901 tccgcagcgc tcgcctcgag aagcctgatc agcgaaccgt agctcacgaa cccctggggc
     3961 tcctccaggg cggcgcgcgt gattcggaac cgcgcgagca aaggctccgc ttgcccgcca
     4021 agctctgcaa cgagctcccg gtagccgctt agagcggagc cctgcaccat cgtcactggg
     4081 cgcccatgag tcgtcaaaag gtaaaagttt gtcgctgaaa gtcaagccag agcggcccga
     4141 tactgtcatc ctccgcgtta tcgattcgct gagggagctc gagcacgcag atgcaagaac
     4201 aagacatcat catcaagaac gggcgggtct tcgacggcaa aggaagcccg gccacgacga
     4261 agcaccttct gatttcgaaa gggtgcgtcg agaagatctc ggacgaagag atccccgcgc
     4321 gcgacgacat caaggtcatc gacgccgatg gccgatgggt gatgccgggt ttcatcgaca
     4381 tccacacgca ctacgacgcc gaggtcgaag cgattccttc gctacacgaa tcgctgcgtc
     4441 acggcgtcac cacggtgttc atggggagtt gctcgttgag cacggcagtc ggcgacccga
     4501 ccgacatcgc agacatgttc acgcgcgtag aggcgattcc ctaccggcac cttctgcccc
     4561 tcatgcaaga ggggaagacc tgggagacgc acgccgagta cgtcgaacat ctcaacagtc
     4621 ttccgctcgg cccgaacgtg gcctcgttcg tcggctactc cgcggttcgt gcgaaggcca
     4681 tgggcctcga gcgcgcgctc gacgcagaag ccaagcccac cgaggcggag ctccagacga
     4741 tggaagaggt gatcgaggag ggctacgatg ccggctttct cggcctttcg ctcaacgggc
     4801 tctactggga caagatgggt ggcgaccgct ttcgcagcaa gtgcctcccc tcgacctacg
     4861 cctcttggaa agagcttcgg cgcatggtac gcggcgtgcg cgagcgaggg ttgaatttgc
     4921 aggcaatccc gaacgtttcg accaagtatc agatcctcat ctacgcggcc ttcagcgcag
     4981 ggctcttcgt tcggaacgcg ctcaagacca gcatcgtctc gatcatggat ctccgctcga
     5041 accgcctgat ctggcgggcg ctgcaggggc ttggctgggc ggtcaacaaa gtattccggg
     5101 ggaagttcag atatcagttc ctcccggtcg cattcgacct ctgggccgat ggtttcgaga
     5161 acgtcgtctt cgaggagttc ggcgcaggcg cagcaggcct tcacctcgag acgatggccg
     5221 agcggcgaaa gctgttccag gacccgagct accgccgtaa gttcaaccgt cagtggaaca
     5281 acccgctgat ccccaaagtc tttcatcgaa atttcgggaa agcgacggtg atggactgcc
     5341 cggacagctc gctcatcgga aagaccttcc gcgagatcgg tgtgctgcag ggcaagtcgg
     5401 aagtcgacgc gtttctcgac ctcttgatcg cctacggcaa cgagctccgc tggtacaccg
     5461 tcaccggcaa tgacaggccc aagccgctcg cgaagatgat gaacagcgag tcgaatctgc
     5521 tgggcttctc agacgccggc gcgcacctac gcaacatggc cttctacaac ttcccgcttc
     5581 gcatgctgaa gttcgtcaag gacgccgagg atcgcggcga accggtcatg cccctcgaaa
     5641 aggccgtcca caagctcacc ggcgagctcg gcgaatggtt ccaaatcgaa gccggcaccc
     5701 tcgaggaggg caagcgcgca gatttggtca tcgtcgaccc agcgcaactc gactaccgcg
     5761 tcgaagagat tcacgaagaa gaaatccccg agctcggcaa ctacgttcgc ctcgtccgcc
     5821 gcaacccgga cgcagtgccg gctgtcatcg tcaacggcac cctcgtcgca gagacgggcg
     5881 aggtcttgcc cgagatcggt cgaacggtgg gcgcggggca gttcctacgc gccagttccg
     5941 cggggcgctc atcccaaacg aagcgcatgc ccaaagaccg cagcgcgtct gagcgtccca
     6001 accaagagca gcagcacatt ccgagtgatc gtgccgcatt cacttccgcg ggaccgtagg
     6061 cgagactttc tgtctcgcag gcgcgtagca ggccagtgct cggtcgacct ttccgtccac
     6121 atcgaagtgg aaggtctcgg cggatgcacc gcgcgggcct cggtagagga gggtgatcgt
     6181 gtcgatccca gtcagcacct cgtctagatc gaagtgaagg tcgggcaagc gggccagcgc
     6241 cttcgtccag tactcgcgca tcgcgggctt gccccgtagc gtcccgctcg gctcgcccgc
     6301 cacgtcgatg atcacaggtg atgaaaactc gaagtcctcg ccatagtgcg ccagcacagc
     6361 gtcgaggtcg cgccggttcc aagcgtcaat ccagtcgcta gcaaacgcgc gcgcgaaatc
     6421 ttgctctacc ctcggcacgc cacgctccgt cagtcgatcg cttcgaagag gcccgccgcg
     6481 cccatgccgc cgccgatgca catcgtgacc accgcgtact tgccgcctcg acgcttgagc
     6541 tcgttgagtg ccgtcg
//
LOCUS       DASRZF010000112         3971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2828013, whole genome shotgun sequence.
ACCESSION   DASRZF010000112 DASRZF010000000
VERSION     DASRZF010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2828013"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="VFG22_05245"
     CDS             complement(<1..756)
                     /locus_tag="VFG22_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6415679.1"
                     /translation="MSPRQRRAWALGWLLAAAFVGLVISLRYLESRYQLLIVDEQLRF
                     ERPWAALLLLAAPLAWFARVWVQRASRPRLQFSRANALRVSKGSWRIRLVDLPSALRT
                     VALALLVIGLAGPQSIHARDTADVSGIEIILTLDMSGSMEAADIRPTRFKATKSVVRD
                     FINRRPNDRIGAVVFGRDAYTLLPLTTDRDALRTALRGLQLDQIDGRGTAIGNAVGVS
                     LNRLKRSRAKSKVIILLTDGESNAGNVSPSQAAD"
     gene            complement(753..1694)
                     /locus_tag="VFG22_05250"
     CDS             complement(753..1694)
                     /locus_tag="VFG22_05250"
                     /inference="COORDINATES: protein motif:HMM:NF039733.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6338 family protein"
                     /protein_id="HET6415680.1"
                     /translation="MKQAAAIVLVSMLFPSFALGQERTVALDAPLETTLGDAIDVLVT
                     VTAAAGDDAAVPEQSFEPFEIISKKASVDPSPNGATQTFTFELQLLCFELGAHELGPI
                     RVRITSAAGELVDIESNTASIEVRSVLANEPDPELKPASQPVTVEQDDYRLLIVLGAL
                     LALAIGALLAWLLMRWWQKRERAEPAPAPPPPPWDTALAELHALERERPAAISEGRTE
                     SWVDAVSDSIRVYLGRRFGFHGLESTTDEIAGALAETKSLRIAPEEAIGFLNQCDLVK
                     FAKASLAEEASQALIEDALGMVDRTRPESVAASGGGS"
     gene            complement(1691..2578)
                     /locus_tag="VFG22_05255"
     CDS             complement(1691..2578)
                     /locus_tag="VFG22_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET6415681.1"
                     /translation="MISRELVKKLKKIEIFTSRLANDQLAGSYHSVFKGRGMAFSEVR
                     QYQAGDDVRFIDWNVSARMNDTYVKVFTEEREMTVMLLVDLSASERFGSVEKPKIETV
                     AEVAALLAFSAIKNNDRVGLILFTDRIERFVPPKKGRSHVMRVVTEILNADPQGEGTD
                     LRVALDLLGSIGKRRTVAFMISDFIAEHYEKPLRVVSKKHDLIPIQIVDPRENELPDV
                     GLALVEDLETGELVEVDTSNLAVRAEYAREAKRQRASREHLFRRLQLDHVTVSTEQDF
                     VRPLTELFRLRQRRLTGYR"
     gene            complement(2591..3604)
                     /locus_tag="VFG22_05260"
     CDS             complement(2591..3604)
                     /locus_tag="VFG22_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET6415682.1"
                     /translation="MSNDVRVINEMVQRESAFIDTVLHEVHKVVVGQDVMIERILIGL
                     LTGGHILLEGVPGLAKTLTVNTICKTIAAKFQRIQFTPDLLPADVIGTVIYNQQRGEF
                     SAKKGPIFANLVLADEINRAPAKVQSALLEAMQELQVTIGDTSHKLDQPFMVMATQNP
                     IEQEGTYPLAEAQVDRFMLHVRVDYPSQDEERKIMDQISDHVLLGRDAGAGSGVQQVA
                     TTAKLLEARKTIGHVYIDPKIKDYIVQIVTATRHPVRVGLGDLTDMIAHGASPRASIA
                     LNVASRAHAFMRHRGYVTPEDIKAIGPDVLRHRIIRSYEAEAEEITSDQIVRRIFETV
                     EVP"
BASE COUNT          709 a         1248 c         1234 g          780 t
ORIGIN      
        1 atccgccgcc tgcgacggtg acacgttccc ggcgttcgat tctccgtcgg tgagaagaat
       61 gatgactttg ctcttggcgc gagagcgctt gagtcggttg aggctgacgc cgaccgcatt
      121 cccaatcgcc gtgcctcgcc catcgatctg atcgagctgg agcccgcgga gcgctgtcct
      181 cagagcatcc cggtccgtcg tgagtggcag cagggtgtac gcatcgcgcc cgaagacgac
      241 cgcgccaatc cgatcgttcg gccgacggtt gatgaagtct cgaaccacgg atttcgtcgc
      301 cttgaagcgt gtgggtcgaa tgtcggcggc ttccatcgag ccggacatgt cgagggtgag
      361 gatgatctcg atgccgctca catccgccgt gtcccttgca tgaatgctct gaggtccggc
      421 gagtccgatg acgagcagcg caagcgcgac ggttcgcaga gcgctgggca ggtcgacgag
      481 acgaatccgc cagcttcctt tggacacccg cagcgcgttc gcccggctga actgaagcct
      541 cggcctcgat gcccgttgga cccacacccg ggcaaaccaa gccaacggtg cggcgagcag
      601 aaggagcgca gcccaggggc gctcgaatcg cagctgctca tcgacaatca gaagctgata
      661 gcgcgattcg aggtatcgaa ggctgatcac cagaccgacg aaagccgcag ctagcagcca
      721 ccccagagcc caggcccttc gctgccgcgg gctcatgacc cgcctccgga cgctgcgacg
      781 ctctcggggc gagttcgatc gaccatcccg agcgcgtctt cgatcagagc ttgcgatgcc
      841 tcttctgcca acgaggcttt cgcgaacttc accaaatcgc actgattcag aaacccaatc
      901 gcctcctcgg gcgcgatgcg aagcgacttc gtctcggcaa gggcgcctgc gatttcgtcg
      961 gtggtgctct cgagcccatg aaaaccgaag cggcggccca agtagacgcg gatcgaatcg
     1021 cttaccgcat ccacccacga ctccgtacgc ccttcggaga tcgcggccgg gcgttcacgc
     1081 tcgagcgcgt gcagctctgc aagagcggtg tcccatggcg ggggcggagg cgctggtgcg
     1141 ggttccgctc gctcgcgctt ctgccaccat cgcatcaaga gccaagcgag cagggccccg
     1201 atggcaagcg cgagcaaggc gcccaagacg atgagcagcc ggtagtcgtc ttgctcgacc
     1261 gtcaccggct gacttgcagg cttcagctct gggtccggct cattggcgag cacggaacga
     1321 acctcgatgc ttgcagtatt cgattcgatg tcgacgagct cgcctgcagc gctggtgatc
     1381 cgtacgcgga tcggaccgag ctcgtgagca ccaagctcga agcaaagcag ttgaagctcg
     1441 aaggtgaagg tctgcgtggc gccgttcggg gaggggtcta cgctcgcttt cttcgagatg
     1501 atttcaaatg gctcaaacga ttgttctgga accgccgcgt catcgcctgc cgccgcggtg
     1561 accgtcacca gaacgtcgat cgcgtcgcct agcgtcgttt cgaggggcgc gtcgagcgcc
     1621 acggtgcgtt cctgccccag cgcgaacgaa gggaagagca tcgagaccaa aacgatcgct
     1681 gccgcttgct tcaccgatag cccgtgagtc gccgttgcct gagccggaag agctcggtca
     1741 aaggccgaac gaagtcctgt tccgtgctca ccgtgacgtg gtcgagctga agtctccgga
     1801 ataggtgctc ccgactcgcg cgctgccgct ttgcttcgcg cgcgtactcg gcccggacag
     1861 ccaggttgct ggtgtcgacc tcgacgagct ctccggtttc caggtcctcg acgagcgcaa
     1921 ggccgacgtc gggaagctcg ttctcccggg gatcgacgat ttgaatggga atcaagtcgt
     1981 gcttcttgga aacgacccgg agcggctttt cgtagtgttc ggcgatgaag tcgctgatca
     2041 tgaacgccac ggtgcgtcgc tttccgatac tgccgaggag gtcgagcgcc acgcggaggt
     2101 cggtgccttc accctgggga tcggcattga gaatctcggt cacgacgcgc atcacgtgtg
     2161 agcgaccctt cttgggtggc acgaagcgct cgatgcgatc ggtgaacaag atcagcccca
     2221 cacgatcgtt gttcttgatc gcgctgaaag cgagcagcgc agccacttcg gcgacggttt
     2281 cgatctttgg cttctcgacc gagccgaatc gctcgctggc agacaggtcg actagaagca
     2341 tcaccgtcat ctcgcgctct tcggtgaaga ccttcacata cgtgtcgttc atgcgcgcgc
     2401 tgacgttcca gtcgatgaag cgaacgtcgt cgccggcttg gtactggcgc acttcactga
     2461 acgccatccc acgccccttg aagacggagt ggtagcttcc ggcgagctgg tcgttggcga
     2521 gcctcgacgt gaagatctcg atcttcttga gtttcttgac gagctcgcga gagatcacga
     2581 tgtgcctcgg ttaggggact tccacggttt cgaagattcg acggacgatc tggtcgctcg
     2641 tgatctcttc ggcttccgct tcgtaggagc gaatgatgcg gtggcgcagc acgtctgggc
     2701 cgatcgcttt gatgtcctcg ggtgtcacat atccgcggtg tcgcatgaag gcatgggcgc
     2761 gggaggcgac gttcagagca atggaggccc gaggcgacgc gccgtgcgcg atcatgtcgg
     2821 tgaggtcgcc gagccccacc cgcaccgggt gacgcgtcgc cgtgacgatc tgaacgatgt
     2881 agtccttgat cttggggtcg atgtacacgt gcccgatcgt cttgcgcgcc tcgagcagct
     2941 tcgctgtggt cgcgacctgc tgcaccccag agccagcgcc cgcatcgcgg ccgaggagca
     3001 cgtggtccga gatctgatcc atgatcttac gctcttcgtc ttggctcggg tagtcgacgc
     3061 ggacatggag catgaagcga tcgacctgcg cctcggcgag cggataggtg ccctcctgct
     3121 cgatcgggtt ctgcgtcgcc atcaccatga acggctggtc gagcttgtgg ctggtatcgc
     3181 cgatggtgac ctgaagctcc tgcatcgcct cgagcagcgc gctttgcacc ttcgcgggag
     3241 cccggttgat ctcgtcggcc agcaccagat tggcgaagat cggccccttc ttggcgctga
     3301 actcaccgcg ctgctgattg tagatcacgg tgccgatcac gtcggcaggc agcaggtccg
     3361 gggtgaactg gatgcgctga aacttggccg cgatcgtctt gcagatcgta ttgaccgtca
     3421 gcgtcttcgc gagccccggt acaccttcga gcagaatgtg cccgcccgtg agaaggccga
     3481 tcaagatgcg ttcgatcatc acatcctggc cgaccacgac cttgtgaacc tcgtgaagaa
     3541 ccgtatcgat gaaggcgctc tcgcgctgaa ccatttcgtt gatgacacga acgtcgttgg
     3601 acatgaaatt tccctcgggt cgcttggcta gcacgctgcc ggacccccca ccaacaagct
     3661 ccggccgcgg cttattcgca aaaaggggca attccccatt tttggtagtc tcagtgtcag
     3721 tgtcgcgcga cagagtgccc ggaggacggc tcgcgacggg cgtgctcgcg attgggaccc
     3781 actcgccccc agttctctaa gacgcccgac gtctaggctc gacccaatcg ctgtgctgcc
     3841 catcccgagc agtcctcctc cgtatgccag tgccagtgcc agcgtcagta ccagcgtcag
     3901 tgtcagtgcc agtgccagcg ccagcgccag tgccagcctc cgcgccactg tccgtctgac
     3961 tctccttggt c
//
LOCUS       DASRZF010000113         2541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2144411, whole genome shotgun sequence.
ACCESSION   DASRZF010000113 DASRZF010000000
VERSION     DASRZF010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2144411"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /gene="aroB"
                     /locus_tag="VFG22_05265"
     CDS             <1..954
                     /gene="aroB"
                     /locus_tag="VFG22_05265"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HET6415683.1"
                     /translation="FDGGADRPWPAEAIRDLTLAGKPPIEVKLPGDEAHKNVASVERV
                     WDTALDAGVDRRAIIVGIGGGVIGDLTAFAASTLLRGVALGQVPTTLLAMVDSSVGGK
                     TGFNRPRGKNLVGTFYQPKFVLCDVETLSTLPSEERIAGLAEVVKSAWLDGEQSVSML
                     ESDAEALMAGDPEASIRAIRMSIALKSRIVHEDEHESGRRMLLNLGHTVGHGLEAASE
                     YRGIRHGEGVALGMLAAMRVAGQMGRGRPEETVRLTRLLERLGLPTDLDRRLNSRVLG
                     FIGSDKKRRGDKIHFVIPRLPGQTEVELLDLEAVKRALVGS"
     gene            1020..1730
                     /locus_tag="VFG22_05270"
     CDS             1020..1730
                     /locus_tag="VFG22_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415684.1"
                     /translation="MSRFILVPMMLAATLAVTVAGCNRFPDLSIQVAANLAPDRTDCG
                     VTDDQDAVLAAGVIDRAAPSVFWSYVITPLVESYIVSNALEFQGQQGNMQITAFEITI
                     VLPDGTLVDLGEGVPNPYKVQTSAVIPANQTPGSVSARATFAVAIPDTFYPAVLSIPE
                     NTGFDSFAIDIRAQGTTLGGFSQTSPAFRWPINLCDGCLGVVCDAPAELGDSAGCYAG
                     QDIWGYCAEIVVPDAFSP"
     gene            complement(1727..>2541)
                     /gene="sppA"
                     /locus_tag="VFG22_05275"
     CDS             complement(1727..>2541)
                     /gene="sppA"
                     /locus_tag="VFG22_05275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HET6415685.1"
                     /translation="SGPFVRAMERLEKDEHVKAVVMRINSPGGSALASDRMWEAARNL
                     GEVKPLIASVGDMAASGGYYIASAADEILAHPNSLVGSIGVVGGKFNFAGLAQEIGVS
                     TYVLQRGKRAAWSTPVRPLSPTERQAFESLLRDTYERFITRVSEGREMEREAVLAAAE
                     GRVMTAQDGLALGLIDEMGGLSEALLKAKTLAGLPIDAPLELWPAPMNLLDAINDLLS
                     GDGDDDAKMLQRIWLRQHPLAASMPLEPWAKVLEIIAKEHAALVPPYFFSLN"
BASE COUNT          462 a          802 c          810 g          467 t
ORIGIN      
        1 ttcgatgggg gcgccgatcg cccatggccc gccgaggcga ttcgggatct gacgctggcg
       61 ggcaagcctc cgatcgaggt gaagcttccg ggcgacgagg cgcacaagaa cgtggccagc
      121 gtcgagcgag tttgggacac cgccctcgat gccggagtgg accgtcgcgc gatcatcgtc
      181 ggaattgggg gcggcgtgat cggggacctg accgcctttg cggcgtcgac cttgcttcgc
      241 ggtgttgctc tggggcaggt tccgaccacg ctcctcgcga tggtcgacag ctcggtcggg
      301 ggcaagacag gcttcaaccg tccgagaggc aagaacctgg tcggtacgtt ttatcaaccc
      361 aagttcgttc tctgcgacgt cgaaacgctt tcgacgctcc cgagcgaaga acgtatcgca
      421 ggcttggccg aagtcgtgaa aagcgcgtgg ctcgatggtg agcaatcggt gagcatgctc
      481 gaatcggacg ccgaggcgct gatggccggt gatccggaag cgagcatccg cgccattcga
      541 atgtcgatcg cattgaagtc ccgcatcgta cacgaagacg agcacgagtc gggacgcagg
      601 atgctgctca acttaggtca caccgtgggc cacgggctcg aagctgcaag cgagtaccga
      661 ggcattcggc acggggaggg cgtcgcactc ggaatgctcg cagccatgcg cgtcgccggt
      721 cagatgggcc gcggaaggcc cgaggagacc gttcggctca cccgcttgct ggagcggctc
      781 ggcttgccca ccgacctaga tcgacgactg aacagccgtg tgctcggctt catcggctcg
      841 gacaagaaac gccgtggaga caagattcac ttcgtaattc cgcgccttcc tggccaaacc
      901 gaggtcgagc tgctggatct cgaagcggtg aagagagcgc tcgttggatc ctaaggtacg
      961 gaaatttggc ggcttccacc cgcgctcgat accatgagaa caccctttgg aggggactta
     1021 tgagccgatt catcctcgta cccatgatgc tagcggcgac gctggcggta acggttgccg
     1081 gctgcaaccg cttccccgat ctgtccatcc aggtggcggc gaatctcgcg cccgaccgaa
     1141 ccgactgcgg cgtgaccgac gatcaggacg cagtgctcgc agctggcgtg atcgaccgtg
     1201 cggctccgtc ggttttctgg agctacgtga tcacgcctct ggtggagagc tacatcgtga
     1261 gcaatgcgct cgagttccaa ggccagcagg gaaacatgca gatcacggcc ttcgaaatca
     1321 cgatcgttct tcctgacggc accttggtcg acctcggcga gggcgtcccg aacccctaca
     1381 aagtgcagac cagcgcggtc atcccggcca accaaacccc aggatcggtt tctgcgcgcg
     1441 caacgttcgc cgtcgccatt cccgacacgt tctaccccgc agtgctttcc attccggaga
     1501 acactggatt cgattcgttc gccatcgaca tccgggctca gggcaccacc ctaggcggtt
     1561 tcagtcagac ctctcccgca tttcgctggc cgatcaacct ctgcgatgga tgtttgggcg
     1621 tcgtctgcga tgcgcctgca gagctcgggg actccgcggg ttgctacgcg ggtcaggaca
     1681 tctggggcta ctgtgctgag atcgtcgttc cagacgcctt ctcaccctag ttcagcgaaa
     1741 agaagtacgg gggaacgagc gctgcatgtt ccttcgcgat gatctcgagg accttggccc
     1801 agggctcgag cggcatggac gccgccagcg ggtgctgccg tagccagatg cgctgaagca
     1861 tcttcgcgtc gtcatccccg tctccgctca agagatcgtt gatcgcatcg agcaggttca
     1921 tcggcgccgg ccagagctcg agcggagcgt cgatggggag ccccgccagg gttttggcct
     1981 tgagcagggc ttcgctgagg ccgcccattt cgtcgatcag gccgagggcc aaaccatctt
     2041 gcgcggtcat cacgcggccc tccgcggcgg cgagaaccgc ttctcgctcc atctcacggc
     2101 cctcggacac cctcgtgatg aagcgctcgt aggtatcgcg gagcaagctc tcgaaggctt
     2161 ggcgttccgt gggggaaagc gggcgcacgg gagtggacca ggcggcgcgc ttgcccctct
     2221 gaaggacgta cgtgctgacc ccgatctctt gggcgaggcc agcgaagttg aacttcccac
     2281 cgacgacgcc gatcgagccg acgaggctgt tggggtgcgc cagaatttcg tcggcggccg
     2341 atgcgatgta atagccacca ctcgccgcca tgtctccaac actggcgatc aagggcttca
     2401 cctcaccgag attgcgcgcg gcctcccaca ttcggtcgct agccagcgcc gaaccgccgg
     2461 gcgagttgat gcgcatgacc accgccttga cgtgctcgtc cttctcgagg cgctccatcg
     2521 cgcgcacgaa aggcccggac a
//
LOCUS       DASRZF010000114        23241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4892379, whole genome shotgun sequence.
ACCESSION   DASRZF010000114 DASRZF010000000
VERSION     DASRZF010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 23241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23241
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4892379"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..538
                     /locus_tag="VFG22_05280"
     CDS             86..538
                     /locus_tag="VFG22_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415686.1"
                     /translation="MLVRGLIVAILGTSISAGATGCADESTVIIPVYDACFTVDECVE
                     TATLCEELAVDFAGFTYSNSICTLTCGAQGSISPDCPIAWVGRFGSCYPSSLAGGIDD
                     TLICFDPCDFDEDCQSGFRCLGPEGLCPRGFESCALDERDAICVPGPY"
     gene            582..2321
                     /locus_tag="VFG22_05285"
     CDS             582..2321
                     /locus_tag="VFG22_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415687.1"
                     /translation="MIFGRSKRLVAWVLPVLAGAILSGCSGRKQVPFGLKDAGAPEVE
                     AVDESADEAGELPVGQTFGPDQVEVPIAESSLVLQSGYALAALQLDLDGSEPIDALVI
                     SATPEEVRVQAAYPRGLDVAARHVDSFLVPSHCVEPVAEIKRVSASLVEARVEHSCET
                     GKRTNLWLLTIEAQPRVRERITILPPNDRSTATIEIEIVVQDQDGDGYEDVVANVRID
                     ENTIPLTWLNRPGGFARDASEPEAALQAIANNAWESLDSDLAGAAKQAHSVLDAFVAL
                     CRESGAARIGLSGTEGLQCGLSPATARAAAVATTAAIRRGSFVQALEIQRWWELTPSE
                     EERARVRAAWRAAKGDGNAVWRTIDHEGAQASLYFRDDDTLVVDGRSPHTVQISSGTK
                     TRLSEAEIVPPAQDPNGRYAVRSVRATCAGFEAEVGPTRGKQTHRIPIERRSDRVPCK
                     TPIDRPASAFEWAVLGWAPQGLVAASGDLLRVVPLDAFAKPAGAPIDLSIGSPLPAPI
                     RGARITPDGSRYVIPHAEGVVIRDWRKGGSGRWLRPENWDAVPGAIRSIALSPNGRQI
                     ALQKGSEIRLLSW"
     gene            2609..3610
                     /locus_tag="VFG22_05290"
     CDS             2609..3610
                     /locus_tag="VFG22_05290"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET6415688.1"
                     /translation="MGDAAHTGRSEHLGPSQRPSPAWTYQTGARVFASPIAGHDGTVY
                     VGSLDGQFVALNPDGSLKWRYSAGQKIYPSALLVGSSVIFGTHQRELVSLSLEGQPRW
                     ELPLGDVVDASATLGPDGRIYVVADGAYAVDLLGRLHWHKPTADHVRSAPVIHPARLI
                     IIGTTEGSLIALRPDGSVAWTLPLGGAIEGAASIDDEGRIYAGTGLGEIVQIDESGTL
                     RWRFKTGDEVRATPAIARDGTVIVGSYDGNVYAISPSGAIRWKVATGGRVRASARIDA
                     DGRIYVGSQDNFLYCIDPDGSVLWRYNVGQDVDSTVEIGSDGTLYFGSDDGGVHALR"
     gene            3616..6021
                     /gene="rnr"
                     /locus_tag="VFG22_05295"
     CDS             3616..6021
                     /gene="rnr"
                     /locus_tag="VFG22_05295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HET6415689.1"
                     /translation="MNEDIPEKDQILEVLRTHAPRAMHVGELCGRLDVPRKLKDQVVR
                     RLLLLSDEKLVRELPGLRFRALESSSNEKRGREKAKTTRGRGRNAKEREVLASPLSPV
                     IEGRLTMTRQGYGFVNVYDGSPDVFIPPDAVGPALHGDTVQIRARPSPKGRDGQIVGI
                     VERRVKEVTGMLQPRGRGMSFEPDDDRLRAPMRVVGKIPKSARKTDVILAEIVTFPQR
                     REDRPEVRVLEVLGAQGVARIEVEKIKIRDGIREAFPDEVEQEAAALPPRVDRDVVKD
                     REDLRAVDLVTIDPETARDHDDAIWVERDGSGFHLIVAIADVSHYVPEGSAMDREAIS
                     RGTSVYLPDRAIPMLPPELSTNLASLVPNRDRLCMAVEMHIDEKGHVSSHRIFEAVMR
                     SHGKLYYEGVARALGLTNQGPHQPTAEKRIETLKILLDLSKRLRARRSKRGALDFDLP
                     EGKIVLDEHGEPKEIVQSRKDPGVRQAYRIVEDMMLVTNETVAAHIKRHAAPGVFRVH
                     GKPDPERISMFCQVAKSFGYDLEEESASTPKQLSRFLQRIEGTDEAPLLRYLLLRAMQ
                     QAIYDTDPTTGHFALAAKDYLHFTSPIRRYPDLMVHRILRSMIRNENLDSASLRPRLQ
                     RLAAQASDSERRAMIVERDVMDVYRTIYMQKHIGDELDGRISGFAPFGIYIQIEDPFV
                     SVLLPFEQLDDTYEPDDLGIRLIGARTSKLFTMNDPVTVRIEDANVQGRDVIGSLLKH
                     QPAKAPTEVRRRQGQSPSRPSGGPKHRSSEPGKKHRSSKGQRGAQDERREQPRKRARR
                     KRR"
     gene            6024..7352
                     /locus_tag="VFG22_05300"
     CDS             6024..7352
                     /locus_tag="VFG22_05300"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HET6415690.1"
                     /translation="MEKTKRTTSVSELIVRKREGDRLTQDEIRSLISGFMDGTVADYQ
                     MSAFAMAVYFKGMDFEETVAMTLAMRDSGEIVNLDSIRLPKVDKHSTGGVGDKVSICL
                     APIAAACGVAVPMMCGRSLGYTGGTLDKLEAIPGFRIDLSIRAFLAQLQKLGCALIGQ
                     TGALAPADKRLYALRDVTGTVESIPLITSSILSKKLAAGIDALVLDVKVGRGAFMRTE
                     SAARELAGSLVRVGRRAGMRVSATLTDMNSPIGYTIGNALETAEAMEILHGRGPDDLR
                     DLTYALVAEMLRMAGISRSEAGALRRINQVVADGTALERMRQIVEAQGGDPAFVDKPS
                     ELPLARHRSTIASPGDGYITGIDPLELGHASMGLGTGRLRVEDVVDPGAGIRLHAQVG
                     DRVRRGDPLATLYASRKGLLKPGAERATACFRIGRRPPPAPSRIIETIRR"
     gene            7828..8073
                     /locus_tag="VFG22_05305"
     CDS             7828..8073
                     /locus_tag="VFG22_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415691.1"
                     /translation="MNSALKHTIIAMATAALSVTAACKSDDGPEAAESTTGAETAAAA
                     EAGAAEASCGEGSCSGAMKEGQAAEASCGEGSCGSEN"
     gene            8167..9009
                     /locus_tag="VFG22_05310"
     CDS             8167..9009
                     /locus_tag="VFG22_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HET6415692.1"
                     /translation="MSQSIHGIGLGLRYDLATELLERNPAAVSWLEIHPENYVNRGGR
                     YEEMLDLARKGWPIVTHGLSTCIGSPEPFDAGYLAELRGFLSDLEIPWHSEHLCLGTV
                     EDRFFHDLLPLPFTDEAAEIASRRLIEVRDAIDRPLALENASYYAPQCADGLEEADFV
                     VEVLERADAKLLLDVNNVYVNSRNFDFDPEAYIDKIPSDRVVQIHVAGHFVRDDGLRI
                     DTHGEPVPDDVYALLDYTLRKVGPVPVLLERDSNVPPLDELLREVDELWNIYRRATGA
                     KHAG"
     gene            8999..9733
                     /locus_tag="VFG22_05315"
     CDS             8999..9733
                     /locus_tag="VFG22_05315"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HET6415693.1"
                     /translation="MLAERAKAMQRVCFDREATPADLELLGSPERWLVYRELVQNRLA
                     QVIGAALTRTKAAIGSEAFGLAVAEWFAAGGPKTRFLRHVPNELADFAIPIWRRVEPP
                     WVAELARFEITSWKVRHGDRDPKSVGELAFDRKPVLSTATEVLRLDYPVHRKPTPGEG
                     YGPDPVVLMVYRDARHQASARELNPLAADLMEAWKRGAETVSESVERVAKAHGTEIGP
                     AFVEKLSALIADSIDRGILLGSGSAQ"
     gene            complement(9727..10872)
                     /locus_tag="VFG22_05320"
     CDS             complement(9727..10872)
                     /locus_tag="VFG22_05320"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6415694.1"
                     /translation="MKSYLFETDEHRALREQVRRFAASAIAPHAHAWEEANEFPRELY
                     RESAKAGFLGTGYPEDVGGDGGDLSHVLVAAEEMVIAGKSVGTCVGLGSHGIALPPIV
                     KFGTDAQKERYVRPTLHGDKISALGITEPGGGSDVAAVLTKAVRDGDHYVVNGAKTFI
                     TSGTRADFVTTAVRTGGEGHGGISMLIIDSDTPGFTVASKLKKTGWWASDTAELAFED
                     CRVPVANLIGQEGAGFLMIMTNFVTERLMLAGQCVAIAELAYRESVAYAKQRHAFGKS
                     LSGFQVVRHKLADMATEIAAARALTGECVTRFLNGEQTPSLAAMAKNTATDMCSSVCD
                     QAVQIHGGYGYMREYVVERLYRDARLYPIGGGTREIMNEIISKVEGY"
     gene            complement(10970..15160)
                     /gene="rpoC"
                     /locus_tag="VFG22_05325"
     CDS             complement(10970..15160)
                     /gene="rpoC"
                     /locus_tag="VFG22_05325"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HET6415695.1"
                     /translation="MKDIFSFFEKPKDPLAFSAIRISLASPEKIREWSHGEVKKPETI
                     NYRTFKPERDGLFCAKIFGPVKDYECICGKYKRMKHRGIVCEKCGVEVIQSKVRRERL
                     GHITLATPVAHIWFLKSLPSRIGAILDITLKDLERVLYCESYVVLNPKETALERGEIL
                     TEDRYHDMLDEYGDDAFDAGMGGEAVLEMLRDLDVHAMAEQLRLDLKEATSEAKRKKL
                     AKRLKVIEAFRDSGNRPEWMMLSVIPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINR
                     NNRLKRLIELNAPEIIIRNERRMLQEAVDALFDNGRRGKTITGPNKRPLKSLSDMLKG
                     KQGRFRQNLLGKRVDYSGRSVIVVGPALRLHQCGLPKKMALELFKPFIYNKLEERGYV
                     TTIKSAKKLVEKEKPEVWDILEEVITEHPVMLNRAPTLHRLGIQAFEPVLIEGKAIRL
                     HPLVCTAFNADFDGDQMAVHVPLSVEAQMETRVLMMSTNNILSPASGRPIINPTQDIV
                     LGLYYMTREKQFGKGAYREGQEKEGQFTGIFASSDEVRMAYDQNMVGLHSRIRYRYKG
                     EIYTTTVGRVLVSDVLPPGMEFPSVNKVLDKKSLSALIDECYRASRNKNTVLLADRLR
                     TMGFEMSTRAGISICMDDMIIPDSKKDVLAAAQEDVTTVVEQYQEGLITDGERYNKVV
                     DIWAEASDQIARDLMESIGNDQVTDPQTGETTTQPSFNSIFMMADSGARGSNQQIRQL
                     AGMRGLMAKPSGEIIETPITANFREGLTVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDCVITDYDCQTLDGIWVSKLEEGGEVVTPLGERVLGRIALEDITDPVTDEA
                     LVEANQEIDEARVARIEAAGIERVLIRSVLTCQNRRGVCVMCYGRDLGRGYEVNIGEA
                     VGIIGAQSIGEPGTQLTMRTFHIGGVAARGKIEQSSIESRSGGIVKFDNAKLVTKRDG
                     KIVVMNRHGAVTVVDESGREREHFTLTYGAFVKVKEGEHVERGTLLAEWDPYAIPILT
                     EVDGVVKYGDVVEAVTMEEKLDEVTGLSRRVIIESRDPSARPRVSIKDPKTGDTRTTG
                     IGENMARYYLPVGANIIPTDGQTVEAGEIIAKIPRETTKTKDITGGLPRVAELFEARK
                     PKDHAIISEIDGIVTFGKDTKGKRKVVIAPEVGEPKEYLIAKGKHLTVKDGDHVRAGE
                     PLMDGPANPHDILKVKGEKELAAWLVDEIQQVYRLQGVAINDKHIEVIVRQMLRRVRI
                     KETGDTRFLPDEQVEKTVFERENERVIAKGGQPAVAEPLLLGITKASLSTDSFISASS
                     FQETTKVLTEAAIGGKVDELRGLKENVIMGRLLPAGTGLGAYNKIDMVVADKALKPLA
                     ATPAILEPEPVAAAASEE"
     gene            complement(15164..19318)
                     /gene="rpoB"
                     /locus_tag="VFG22_05330"
     CDS             complement(15164..19318)
                     /gene="rpoB"
                     /locus_tag="VFG22_05330"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET6415696.1"
                     /translation="MAATIQTNFRMRHSFGKIEHILEIPNLIDIQKRSYDKFLQTDVS
                     RDDRENTGLQGVFNSVFPIRDFNGTSELVFSGYTLERPKYDVDECRQRGMTYAAPIKV
                     TIQLIIYDAGGDSTERAVRDIKEQEVYFGEIPLMTVNGTFIINGTERVVVSQLHRSPG
                     VFFDHDRGKTHSSGKLLYQARIIPYRGSWLDFEFDPKDLLYVRIDRRRKMPATVLLKA
                     LGYNTQQLLNYYYDTETIYILSKDQYAKSIEYDLLPGQRATHDISVGDDVIVRKNRKF
                     TRAAIRKLKSAGLDYLPIDFTEVIGKVAAEDVVDEETGEILLAVNEEVTEARIDALRE
                     AGVNEFKVLFIDGLNVGSYLRDTLLVDKVQSQDEGILEIYRRLRPGDPPTLDTARNLF
                     NNLFFNPERYDLSQVGRLKLNYKFYKDVEPEERPALDHTVLTNTDILDTVKNLIELKN
                     GRGSVDDIDHLGNRRVRAVGELMENQYRIGLVRMERAIKERMNMSQEIETLMPHDLIN
                     AKPVSAVVKEYFGSSQLSQFMDQTNPLSEVTHKRRLSALGPGGLTRERAGFEVRDVHT
                     THYGRICPIETPEGPNIGLIASLSTYARVNEYGFVETPYRKVEDGIVLDDHVRWYSAL
                     EEEGHYIAQANAKIAHVSRKLEGQLISARFNGEFVMVPPSSVQLMDVSPNQLVSVAAS
                     LIPFLEHDDANRALMGSNMQRQAVPCVRTHAPIAGTGIERRVARDSGVCVVAQQDGVV
                     ESVDATRIVVKAEGLAGAFPDIYRLNKFQRSNQNTAYNQTPIVRPGDAVKAGDVIADG
                     PSTDRGELALGQNVIVAFMPWGGYNFEDSILLSERIAKDDVYTSVHIEEFECVARDTK
                     LGKEEITRDIPNVGEEALKDLDESGIVRIGAEVSSGDILVGKITPKGETQLSPEEKLL
                     RAIFGEKAGDVRDTSLKVPPGVTGIVIDARVFARKGTEKDERSLQIEESERAKLEKDM
                     ADEIKIVLDSAHDRVRKYFVGSTATAKLVGDKGAELLTKGQKITDGDLDGIPHKYWGH
                     IEIDKDKDKTDAILEQVDDQVDAVKMLFQEKIDKLGKGDELPPGVIKMVKVYVAIKRK
                     LQVGDKMAGRHGNKGVVSRILPEEDMPYLPNGKPVDIVLNPLGVPSRMNVGQILETHL
                     GWAGYLLGTQLQEMVEQKASAAELRERIRSIFKGGDIVKVLDTLPDGEVAKMAKSWKH
                     GLQLATPVFDGASEKEIKELLKLAGVQESGQTVLFDGRSGEAFHEDVTVGIMYMLKLH
                     HLVDDKIHARSIGPYSLVTQQPLGGKAQFGGQRLGEMEVWAMEAYGAAYALQEFLTVK
                     SDDVQGRTRMYESIVKGDYSLEAGLPESFNVLMKELQALCLNIELVETSELERRSDDV
                     LAAEEE"
     gene            complement(19536..19910)
                     /gene="rplL"
                     /locus_tag="VFG22_05335"
     CDS             complement(19536..19910)
                     /gene="rplL"
                     /locus_tag="VFG22_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017150119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HET6415697.1"
                     /translation="MNLEQMVEELSSWTVMEVANLVKQLEETWGVSAAPAAVAVAGPA
                     GGGAAEAVEEQTEFDVELTEAGGKKINVIKAIREITQLGLADAKALVEAAPKVVKEAV
                     SKEEAADIKKKLEEAGAKVTVK"
     gene            complement(19971..20540)
                     /gene="rplJ"
                     /locus_tag="VFG22_05340"
     CDS             complement(19971..20540)
                     /gene="rplJ"
                     /locus_tag="VFG22_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HET6415698.1"
                     /translation="MSLTRQEKTAQAASVRESFDTASATVLVDFRGVNVELITNLRSR
                     FREAGVEYKVVKNNVVRKALEGSALAENDEFTAYLAGPTAIAWSFEDPSAAAKVIKSF
                     RKEFTDILQPKDGKEKLLVKCGLIEGQVLSAKRVENELATLPGKDEIRASLLAQLMAP
                     MQKLVGQLNAPGQNLALVLDAKRRQAAGE"
     gene            complement(20543..21241)
                     /gene="rplA"
                     /locus_tag="VFG22_05345"
     CDS             complement(20543..21241)
                     /gene="rplA"
                     /locus_tag="VFG22_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HET6415699.1"
                     /translation="MKPGKKRAGALQKPDKSRRYALQEAVSLVKELAFANFDESVDVA
                     VRLGVDPKHADQMVRGAVVLPHGTGKTNRVLVFAKGEKVSEAEAAGADFMGGDDMVAK
                     INEGWLDFDTVIATPDMMGQVGRLGRVLGPRGLMPNPKVGTVTFDVAKAVSDAKAGKV
                     EFRAEKAGIVHAQVGKRSFGEGELAGNVRSLVGALVKAKPAAAKGVYLRSITLSSTMG
                     PGIKVDPGTIEAEA"
     gene            complement(21329..21751)
                     /gene="rplK"
                     /locus_tag="VFG22_05350"
     CDS             complement(21329..21751)
                     /gene="rplK"
                     /locus_tag="VFG22_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HET6415700.1"
                     /translation="MKKVIGQIKLQLPAGKANPSPPVGPALGQHGVNIMAFCKEFNAK
                     TQKDAGLIIPVVISVYQDRSFSFIMKTPPAAVLIKKALNLESGSARPNKDKVGKITRD
                     QVMEIAKVKIQDTNAGSLETCMQSIIGTARSMGVELVD"
     gene            complement(21872..22396)
                     /gene="nusG"
                     /locus_tag="VFG22_05355"
     CDS             complement(21872..22396)
                     /gene="nusG"
                     /locus_tag="VFG22_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HET6415701.1"
                     /translation="MKWYVVQAYSGYENKVKLSLEERIKQAGMEGRFGNILIPKENVQ
                     ESRGGKRRLSSRTFYPGYIFVQMDLDDQTWHLIKDTPKVNGFVGGRHPSPVPEPEIAA
                     IEQQVEEGAAKPKPRVQFEKGDHVRVTDGAFANFTGAIEEVNHEKHKVRVLVSIFGRA
                     TPVELDYAQVEKTN"
     gene            complement(22402..22827)
                     /gene="secE"
                     /locus_tag="VFG22_05360"
     CDS             complement(22402..22827)
                     /gene="secE"
                     /locus_tag="VFG22_05360"
                     /inference="COORDINATES: protein motif:HMM:NF012793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HET6415702.1"
                     /translation="MAITNEQDKARPTGTLGVERWVQFAYAACAITLAWFLIKCSNAI
                     WTMLADSVDAIPEANATAIAFGAGLVAFISAVVAYRSKKVHTFVVEVCVELSKVSWPT
                     RKETWSQTIVVLIVSVIAAIILGVYDAVWSHITDLIYNV"
     gene            complement(22843..22916)
                     /locus_tag="VFG22_05365"
     tRNA            complement(22843..22916)
                     /locus_tag="VFG22_05365"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22880..22882),aa:Trp,seq:cca)
     gene            complement(22928..23089)
                     /gene="rpmG"
                     /locus_tag="VFG22_05370"
     CDS             complement(22928..23089)
                     /gene="rpmG"
                     /locus_tag="VFG22_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003829846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET6415703.1"
                     /translation="MSDRRVKIALACDECGTRNYQTTKLRKAGQQERLTLKKFCPKCN
                     RRTDHKETK"
     gene            complement(23082..>23241)
                     /gene="tuf"
                     /locus_tag="VFG22_05375"
     CDS             complement(23082..>23241)
                     /gene="tuf"
                     /locus_tag="VFG22_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008426562.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET6415704.1"
                     /translation="ELPEDVKMVMPGDNVKMKVNLITPAAMEEKQRFAIREGGRTIGA
                     GVISKIIE"
BASE COUNT         4383 a         7074 c         7049 g         4735 t
ORIGIN      
        1 agatgaaaga agaagcggaa gaagaagggc tccttggacc tggtgcaggg cggccgcaac
       61 gacaaacccg agtggttgaa ttgacgtgct tgttcgcggc ctgattgtgg cgatcttggg
      121 cacgtcgata tccgcgggtg caacaggctg tgccgacgag agcacggtca tcatcccggt
      181 ctacgacgcg tgcttcacgg tcgacgaatg cgtcgagacc gcaacgctct gcgaggagct
      241 cgcggtcgac ttcgctggct tcacctattc gaattcgatc tgtacgctca cctgtggagc
      301 ccaaggatcc atttccccgg actgtccgat agcctgggtg ggccgctttg gaagctgcta
      361 cccgtcgagt ctcgctggcg gaatcgacga tacgctgatc tgcttcgacc cttgcgactt
      421 cgatgaggac tgccagtcgg gattccgctg cctgggtcca gaagggctct gtcctcgggg
      481 gttcgagtcc tgcgccctcg acgagagaga cgcgatttgc gtacccggtc cgtattgaca
      541 cttttgaggc tccccagccg aacgcatgct aatccgaacg catgattttt gggcgatcta
      601 agcggctggt agcgtgggtc ctcccagttc ttgctggggc aattctcagc ggctgctccg
      661 ggcgcaagca agtcccgttc ggactcaaag acgcaggagc tcccgaggtc gaagcggtgg
      721 acgagtcggc cgacgaagcg ggcgagcttc ccgtgggtca aacgtttggt ccggatcaag
      781 tggaggtgcc gatcgccgag tcgtcgctcg tcctgcaatc cggatatgca ctggccgcgc
      841 ttcaactgga tttggacggc agcgagccca ttgacgcgct cgtgatctca gcgacgcccg
      901 aggaagtccg cgttcaggcc gcctatccgc gcggcctcga cgttgctgct cgtcacgtgg
      961 attcgttcct ggtccccagc cactgcgtcg aacccgtcgc agagatcaaa cgagtctcgg
     1021 cctcgctcgt ggaagcccgc gtcgaacaca gctgcgagac cgggaagcga acgaacctct
     1081 ggctgctcac gatcgaggct cagccacgtg tgcgcgagcg aatcaccatt ttgccaccga
     1141 acgaccgctc gacagcgacg atcgagatcg agatcgtcgt gcaagatcag gatggcgacg
     1201 gttacgagga cgtcgtcgcc aacgtccgaa tcgatgagaa cacgattcca ctgacgtggc
     1261 tgaacaggcc cggcggtttt gcgagagatg cctccgagcc agaagccgca ctgcaagcga
     1321 ttgcgaataa tgcctgggag tccctcgact ccgacctcgc aggtgctgcc aagcaagcgc
     1381 actccgtcct cgacgcgttc gttgcgctct gccgtgaaag tggggcagcc cgaatcggac
     1441 tgtccggcac cgaaggcctg caatgcggcc tttccccagc gaccgcccgg gcagccgcgg
     1501 tcgctacgac tgcagcaatt cgacgaggta gcttcgtgca ggcgctcgag attcagcgct
     1561 ggtgggagct tacccccagc gaggaagagc gggcgcgggt gcgcgcagcc tggcgggcgg
     1621 cgaaggggga cgggaacgcc gtctggcgaa ccatcgacca tgagggcgct caagcgtccc
     1681 tgtactttcg cgacgacgat actctggtcg tcgacgggcg ctctccccac acggttcaga
     1741 tcagctcggg aaccaagacg cgactgtccg aggcggagat cgtgccaccc gcgcaagatc
     1801 ccaatggccg ctatgcggtg agaagcgtcc gcgcgacctg cgcgggcttc gaagcggagg
     1861 tgggtcctac acgcggcaag caaacacacc ggattccgat cgagcgtcgc tccgatcgag
     1921 tgccttgcaa aacaccgatc gaccgacccg catcggcgtt cgagtgggcg gtgcttggct
     1981 gggcccctca gggcctggtt gccgcttccg gcgacttgct tcgcgtcgtt ccgctcgatg
     2041 cgtttgccaa accagcggga gctcccatcg acctgagcat tggcagcccg ctccctgcgc
     2101 cgatcagggg cgcacggatc acacccgatg gatcacgcta cgtgattccg catgcggagg
     2161 gagtcgtgat ccgcgactgg cgcaagggtg gatcggggcg ctggttgcgc cctgagaatt
     2221 gggacgctgt gcccggcgca atccggtcca tcgccctgag ccccaatggc cgccaaatcg
     2281 cgctccaaaa aggtagcgag atcaggcttc tcagctggtg atcaagtcga ctgcgacgat
     2341 gaatcggcga tggtatagtc gcagcgcctt gcgctactgc ctgcaactct ttttggcatt
     2401 cgccacgttc atctcgatct cggcctgtag cggtgctcgc ccgcggccat cgaccccgtg
     2461 catgtcggat accgaatgcc gtggggaccg gatttgtcat gaagggaggt gccgcttcgt
     2521 cgaggaggtg ctctccgagc tggccagccc gcctgccata ccggacgcag tcaaaccggg
     2581 cgtcccacca cggagtgcca acggattcat gggtgacgcc gctcatacgg ggcgcagtga
     2641 gcacctcggc ccatcgcaac ggccctctcc tgcctggacc tatcaaaccg gagcgcgggt
     2701 cttcgcttcg cctattgccg ggcatgacgg gaccgtgtac gtgggctctc tcgacgggca
     2761 gttcgtagcc ctgaacccag acggatcgtt gaaatggcgc tactcggccg gacaaaagat
     2821 ctacccgagc gccctgctcg tcggatcgtc tgtgattttt ggaacccacc aacgggagct
     2881 cgtttcgttg tcgctcgagg gccagccccg atgggagctt ccgctcgggg acgtggtcga
     2941 tgcatcggcg accctcggtc cggacgggcg catctacgtg gtagcggacg gagcgtatgc
     3001 ggtagacctc ctgggccgat tgcactggca caaaccaacc gcagatcacg tgcggagcgc
     3061 accggtcatc cacccagcgc gcttgatcat catcggcacg acggaaggct cgctcattgc
     3121 ccttcgaccc gatggctcgg tggcttggac cctgccgctc ggcggagcca tcgagggcgc
     3181 agcctcgatc gacgatgaag ggcggatcta cgccggcacg ggcctgggcg agatcgtaca
     3241 gatcgatgaa tccggcacgc ttcgctggag attcaagact ggtgacgagg tgcgggccac
     3301 cccggcaatc gcccgagacg gcaccgtcat cgtcggctcg tatgatggga acgtctatgc
     3361 cataagccct tccggcgcca tccgttggaa ggtagccact ggaggccgag tacgagcctc
     3421 ggcccgcatc gacgcggatg gtcgcatcta cgtgggctca caagacaact tcctctactg
     3481 catcgacccg gacggctctg tgctctggcg atacaacgtt gggcaggacg tcgactcgac
     3541 ggtagagatt ggatccgatg gaacacttta cttcggatcc gacgacggag gggttcatgc
     3601 cctccgatag agatgatgaa cgaagatatt cctgaaaaag accaaattct agaagtactg
     3661 cggacacatg ctcctcgtgc aatgcacgta ggcgagctct gcggacgact cgatgttccg
     3721 cgaaagctca aagaccaagt cgtccggcgc ctactgttgc tgtctgacga gaagctcgtt
     3781 cgggagctac cgggattgcg gttccgcgcg ctcgaatcgt cgagcaatga aaagcgtgga
     3841 cgcgagaagg ccaagaccac gcggggccgc ggacgaaatg cgaaagaacg cgaagtcctc
     3901 gcttcccccc tcagtcccgt catcgagggc cgcctgacga tgactcgaca agggtacggt
     3961 ttcgtcaacg tgtacgacgg aagccccgac gtgttcattc cgcccgacgc tgtgggtcct
     4021 gcgctgcatg gggacacggt ccaaattcgt gctcgaccct cgcccaaggg ccgggacggt
     4081 cagattgtcg gcatcgtcga gcgccgggtg aaagaagtga ccggaatgct tcagcctcgg
     4141 ggtcgcggga tgagcttcga gcccgacgac gaccgcttgc gcgcgccgat gcgcgtggtc
     4201 ggaaagatcc cgaaatccgc tcgcaagacc gatgtgattc ttgccgagat cgtcacgttt
     4261 ccgcaacgcc gtgaagatcg acccgaggtt cgcgtcctcg aggtgctcgg tgcccaaggg
     4321 gtggctcgga tcgaagtcga gaagatcaag attcgcgatg gcattcgaga agcctttccc
     4381 gacgaagtcg aacaggaggc ggcggctttg cctccacgcg tcgatcggga tgtcgtcaaa
     4441 gaccgagaag acttacgggc cgttgacttg gtcacgatcg accccgagac ggcacgcgac
     4501 cacgacgatg cgatttgggt cgagcgcgac ggtagtggat tccatctcat cgtcgccatc
     4561 gccgacgtgt cgcactacgt ccccgaagga agcgccatgg accgcgaggc gatcagccgc
     4621 ggaaccagcg tctacctgcc ggatcgtgcg atccccatgc tgcccccgga gctctcgacc
     4681 aacctggcct cgctcgtacc caaccgcgat cgcctgtgca tggcggtcga aatgcacatc
     4741 gatgaaaagg gacacgtatc ttcgcatcgg atcttcgagg ccgtgatgcg gtcgcacggc
     4801 aagctctact acgagggcgt ggctcgtgcg ctcggcctaa cgaatcaagg tccccaccaa
     4861 ccaacggccg agaaacgaat cgaaacgctg aagatcctgc tcgacctatc aaagcgactt
     4921 cgggcgcgac ggtcgaagcg tggcgcactc gatttcgatc tccctgaagg aaagatcgtc
     4981 ctcgacgaac acggagagcc gaaggagatc gttcaatcgc gaaaggaccc aggggttcgt
     5041 caggcttatc gcatcgtcga agacatgatg ctggtgacga atgaaaccgt agcggcgcac
     5101 atcaagcggc acgcagcgcc cggggtattc cgggtgcacg ggaagccgga tcccgaacgc
     5161 atctccatgt tctgccaggt ggcgaaatcg ttcggctacg atctcgaaga agagtcggcg
     5221 tcgacaccga agcaactgag tcgttttctc caaagaatcg aagggacgga cgaagcgccc
     5281 ctgctccgat acttgctcct gcgagcgatg cagcaggcga tttacgatac cgatccgacg
     5341 accggtcact ttgctctagc ggccaaggac tacctacact tcacctctcc gatacgacgc
     5401 tatcccgact tgatggtgca tcgcatcctc cggagcatga ttcgcaacga gaatctcgat
     5461 tccgcctcgc ttcgacctcg tctgcaacgc ctcgcggcgc aagctagtga ttccgaacgg
     5521 cgtgcgatga tcgtcgaacg cgacgtcatg gatgtctacc gcacgatcta catgcaaaag
     5581 cacatcggag acgagctcga cggacgaatt tccggatttg ctcctttcgg tatctacatt
     5641 caaatcgaag atccattcgt gagcgtcctg cttccgttcg agcaactcga cgacacatac
     5701 gaaccagatg atctcggcat ccgcttgatt ggcgcgcgga cttcgaagct cttcacgatg
     5761 aatgatcccg tcaccgtgcg gatcgaagac gcgaacgttc agggccgcga cgtcatcggg
     5821 tctctgctga agcatcagcc ggcgaaagca ccgaccgagg tacggcgcag gcagggccag
     5881 tcaccgagca gaccaagcgg cggcccgaag caccgcagct cggagcccgg caagaaacac
     5941 cgcagctcga aggggcagcg cggcgctcag gacgaacgtc gcgaacagcc tcgcaagcgg
     6001 gcgcgacgca aacggcgctg acgatggaga aaacgaaacg aacgaccagc gtatcggagc
     6061 taatcgttcg caagcgcgaa ggcgaccggc tgacccagga cgaaattcgg agtctgatct
     6121 ctggtttcat ggacgggacg gtcgccgact atcaaatgag cgcgttcgcg atggccgtct
     6181 acttcaaggg catggatttc gaagagacgg tcgcgatgac actcgcgatg cgagacagtg
     6241 gagagatcgt caacttggac tcgatccgcc tcccgaaggt ggacaaacac tccaccggag
     6301 gtgtcgggga caaagtcagc atctgccttg caccgattgc ggctgcctgc ggggttgccg
     6361 tgcccatgat gtgcggaaga agcctcggtt acacgggagg cacgctcgac aagctcgaag
     6421 cgattcctgg gtttcgtatc gacctttcaa ttcgcgcatt tcttgctcag cttcagaagc
     6481 tcgggtgcgc actcatcgga caaaccggcg ccttggcccc ggctgacaag cgcttgtacg
     6541 cccttcgaga cgtgacgggg accgtcgaat cgattccatt gatcacctcg agcatccttt
     6601 cgaagaagct tgcggcaggc atcgacgcgc tggtgctcga cgtcaaggtc ggccgcggcg
     6661 cgttcatgcg gaccgagtct gctgcgaggg agctcgcagg tagcctcgtg cgggtgggcc
     6721 gacgcgcggg gatgcgcgta tcggcgacgc tgaccgacat gaacagcccc atcggataca
     6781 cgattggcaa cgcgctcgaa acggccgaag cgatggagat tctacacggc cgcggacccg
     6841 atgatctcag ggacctgacc tacgctttgg tggccgagat gcttcgcatg gcgggaatca
     6901 gcaggagcga ggcgggtgcc ctgcggcgaa tcaaccaggt agtggctgac ggaacggctc
     6961 tcgagaggat gcggcaaatc gtcgaagcgc aaggcggcga cccagcattc gtcgacaagc
     7021 caagcgagct cccgctagca cggcatcgct cgacgatcgc gtcgccgggc gatggctaca
     7081 tcacgggtat cgatccgctc gagctcgggc atgcatcgat gggcttggga acagggcgac
     7141 tccgcgttga agacgtggtc gacccgggtg ctggaatccg actgcatgct caggtcggcg
     7201 accgagtccg cagaggcgat ccgctcgcca cactctatgc atcgcgaaag gggctgctga
     7261 agccgggcgc agagcgagcc acggcttgct ttcgaatcgg ccggcgacct ccgccggcgc
     7321 cgagccgcat catcgagacg attcggcgct gacggccggc tcggctatcg ccgatcggaa
     7381 ccaactcacg cagcgcgatg ttcgcgaagc atgcgcgaac acgagcacga cgaaagttgt
     7441 caacgaatgt aacggcatag tgcgcggtcc cacccaaagc atcactgctt gggagtgtcg
     7501 ggggagcctg tccgcaggcg gcaaggccca gcacagctgc gaatttccaa gatcctttga
     7561 cgatctaagc ctctggaatc cgtggggtat tcgttgcgag ttgccagtgc ttgggatttg
     7621 aatttcaagc gaaacatcta agcgtcacga gcgtatctcg gtcgtgacgc aggcttatca
     7681 tttagattgc tacacgcgat cgctttggct agcgtgcccc aatagggaag cggggttacg
     7741 ggattggtcc ctgacgccgc gttgcatcac cggggacctc catggatgct cccgatgcgc
     7801 ttacatacac aaagagggta gacaaacatg aactcagcac tcaagcacac gatcatcgca
     7861 atggcgactg cagcgctcag cgtgaccgcc gcatgcaagt ccgacgatgg tccagaagcc
     7921 gccgagtcga cgacaggcgc agagaccgct gctgcggctg aagcaggcgc ggccgaagct
     7981 tcttgtggcg aagggtcctg cagcggcgcc atgaaggaag gccaggccgc cgaggcgtcc
     8041 tgtggcgaag gcagctgcgg cagcgagaac tagaacagct cgaccacgcc gccaacactc
     8101 gagcggtatg gtgttgcgag ccgcggcggc attggtcgcc gcggcttctt tttggtctga
     8161 actgagatga gtcaaagtat tcacggaatc gggctcggcc tacgctacga cctcgcgacc
     8221 gagctcttgg agcgcaaccc tgcggccgtg tcctggttgg aaatccaccc ggagaactac
     8281 gtgaatcgag gcggccggta cgaggaaatg ctcgacttgg cgcgcaaagg gtggccaatc
     8341 gtaacccacg gactgagtac ctgcataggg tcgcccgaac cgttcgacgc cggctatttg
     8401 gcagagctac gaggcttctt gtcggacctc gagatccctt ggcatagcga gcatctctgt
     8461 ctcggcacgg tggaggaccg cttctttcac gacttgcttc cgcttccctt taccgacgag
     8521 gcggcggaga tcgcctcgcg ccggttgatc gaggtgagag acgcgatcga ccgacccttg
     8581 gccctggaga atgcgagcta ctacgccccg caatgcgccg acggtctgga ggaggcggat
     8641 ttcgtcgtcg aggtactcga acgcgcagat gcgaagctcc ttctcgacgt gaacaacgtc
     8701 tatgtgaaca gccgcaactt cgacttcgat cccgaggcgt acatcgacaa gatcccctcg
     8761 gaccgcgtgg tgcagatcca cgtcgctggc cacttcgttc gcgacgacgg gctgcgaatc
     8821 gatacccatg gggagccagt gccggacgac gtttacgcgc tgctcgacta caccttgaga
     8881 aaggtcggcc ctgtcccagt gctcctcgag cgagacagca atgtgccgcc gctcgacgag
     8941 cttctccgag aggtggacga gctctggaac atctatcgac gcgcgacagg ggccaagcat
     9001 gctggctgag cgcgccaagg ccatgcagcg cgtctgcttc gatcgggaag cgaccccagc
     9061 cgacctcgag ctgctgggat caccggaacg ctggctcgtc tatcgagagc tcgtgcaaaa
     9121 ccggctcgct caggtgatcg gtgcggcgct cacccgaacg aaggcggcaa tcgggagcga
     9181 ggctttcggg ctcgctgtcg cggagtggtt tgcggccggc ggaccgaaga cccgattcct
     9241 gcggcacgtt ccgaatgagc tcgccgactt tgcgatcccg atttggcgac gcgttgaacc
     9301 tccatgggtt gcagagctcg cgcgtttcga gatcacgagc tggaaggtgc ggcatgggga
     9361 cagggatccc aagtccgtag gtgagctcgc cttcgacagg aagccggtct tgagcacggc
     9421 aaccgaggtg ttgcgtctcg actatccggt ccaccggaaa ccgaccccgg gcgaggggta
     9481 cgggccggat ccggtcgtcc tcatggtgta tcgggatgct cgccatcagg cttccgcacg
     9541 tgagctcaat ccattggccg ctgatctcat ggaggcctgg aagcgcggcg ctgaaaccgt
     9601 gtccgaatcg gtcgagcgag tcgcaaaggc ccatggcact gaaatcgggc ctgccttcgt
     9661 ggagaagctg tcagccctca tcgcggactc gatcgaccgg ggcattttgc tcggcagcgg
     9721 aagcgctcag tagccctcaa ccttgctgat tatctcattc atgatttcgc gcgtaccccc
     9781 tccaatggga tagaggcgcg catctcgata gaggcgctct acgacatact cgcgcatgta
     9841 tccgtagccg ccatggatct gtacggcctg atcgcagaca ctcgagcaca tgtcagtggc
     9901 cgtgttcttg gccatcgctg ccaatgaagg agtctgctcg ccattgagaa accgggtcac
     9961 acactccccg gtcagcgcgc gcgcggctgc gatttcggtt gccatgtcgg cgagcttgtg
    10021 gcggacgacc tggaatcccg agagcgactt accaaacgcg tggcgctgtt ttgcgtacgc
    10081 aaccgactca cggtaggcga gctcggctat ggcgacgcac tgaccagcga gcatgaggcg
    10141 ctcggtcacg aagttggtca tgatcatcaa gaagccagcc ccttcctgcc cgatgagatt
    10201 cgcgactggc actcgacaat cttcgaaagc gagctccgca gtgtcgctcg cccaccagcc
    10261 ggtctttttg agcttgctcg ccaccgtaaa tcctggggtg tcgctatcga tgatgagcat
    10321 cgaaatgccg ccgtgtccct cgcctccggt gcgtacggcc gtggtcacga agtctgcacg
    10381 ggtgccggag gtaatgaagg tcttggcgcc gttcacgaca tagtggtcgc catcacggac
    10441 cgccttcgtg agcacggcag cgacgtcact gccgccgccc ggctcggtaa tgccaagtgc
    10501 ggagatcttg tctccgtgga gcgtggggcg aacgtaacgc tctttctgtg cgtcggtgcc
    10561 gaacttcacg atcggtggca atgcgatacc gtgactgccg aggccaacgc aggttcccac
    10621 tgacttgccg gcgatgacca tctcttctgc ggccaccaag acgtggctca gatccccgcc
    10681 atcaccgccc acatcttcgg gatacccggt tccgaggaag cccgctttcg cggactctcg
    10741 atagagctcg cgcggaaact cgttggcttc ttcccaagca tgcgcgtggg gcgcgatcgc
    10801 gctagccgcg aaacgcctca cctgctcacg aagcgcacgg tgctcgtctg tctcgaatag
    10861 gtagctcttc atcgcccttc tccgcttggc taactctaca cggggatgca gtcccgagtt
    10921 agacgaaatt cggatcggga agtcggggcg tttcgccgcc ccgacttagc tattcctcgg
    10981 acgcggcagc agcaacgggc tccggctcta ggatggccgg cgtcgcagca agcggtttga
    11041 gtgctttgtc cgcgaccacc atatcgatct tgttgtaggc gcccagaccg gtaccagccg
    11101 gaagcagtcg acccatgatg acgttctctt tgagaccccg cagctcgtcg accttgccgc
    11161 cgatggctgc ctccgtgagc accttcgtcg tctcctggaa cgaagaggca ctgatgaacg
    11221 agtccgtcga aagcgacgcc ttcgtgattc cgagcaagag cggctccgcg acagcaggct
    11281 gcccgccctt cgcgatcacg cgctcattct cgcgctcgaa taccgtcttt tcgacttgtt
    11341 cgtctggaag gaagcgggtg tcgcccgtct ccttgatgcg aacgcgtcga agcatctggc
    11401 gaacgatgac ctcgatgtgc ttgtcgttga tggcaacacc ctgaagtcgg tagacctgct
    11461 ggatctcgtc gaccagccag gccgcgagct ctttctcgcc cttgaccttg agaatgtcgt
    11521 gtggattggc cggtccatcc atcagaggct cgccggcgcg cacgtggtca ccgtccttca
    11581 cggtgaggtg cttgcccttg gcgatgagat actccttggg ctcacctacc tccggggcaa
    11641 tcacgacctt gcgcttgccc ttcgtgtctt taccgaaagt gacgatgccg tcgatctcgc
    11701 tgatgatcgc gtggtccttc ggcttgcgag cctcgaagag ctcggcgacg cggggcagac
    11761 caccggtgat gtccttggtc ttggtggtct ctcggggaat cttggcgatg atctcaccag
    11821 cctcgacggt ctgaccatcc gtggggatga tgttcgcgcc aacgggcagg tagtatcggg
    11881 ccatgttctc gccgatgccc gtggtgcggg tgtcgcccgt tttcggatcc ttgatcgaaa
    11941 cgcggggtcg cgcgctcggg tcacgagact cgatgatgac gcgacgggaa agaccagtca
    12001 cctcgtccaa cttctcttcc atcgtgaccg cttcgacgac atcaccgtat ttcacgacgc
    12061 cgtcgacctc ggtcagaatc ggaatggcat aggggtccca ctcggcgagc agcgtgcctc
    12121 gctcgacgtg ttcgccctct ttgaccttca cgaatgcgcc gtaggtcagc gtgaaatgct
    12181 cgcgctcacg acccgactcg tcgacgaccg ttacggcgcc atgacggttc atgacgacga
    12241 tcttgccgtc ccgcttcgtc acgagcttgg cgttgtcgaa cttcacgatg ccgccggagc
    12301 gcgactcgat ggagctctgt tcgatcttgc cgcgagccgc gacaccgcca atgtggaacg
    12361 tacgcatcgt gagctgcgtt ccgggctcac caatcgactg cgcaccgatg atgcccaccg
    12421 cctcgccgat gttcacttcg tagcctcggc cgaggtcgcg cccgtaacac atcacgcata
    12481 ccccacgacg gttctgacag gtcaacaccg agcgaatcag cacgcgctcg ataccggcag
    12541 cttcgattcg cgcgacgcga gcctcatcga tttcctggtt ggcttcgacg agagcttcgt
    12601 ccgtgaccgg atcggtgatg tcttcgagtg cgattcggcc gaggactcgc tcacccagag
    12661 gtgtgacgac ctcgccgccc tcttcgagct tgctcaccca gataccgtcg agtgtctgac
    12721 aatcgtagtc ggtgatgacg cagtcctgcg caacgtccac gagacgacgg gtcagatagc
    12781 ccgagtttgc cgtcttcaag gccgtatcgg cgagaccttt acgcgcaccg tgtgtcgaga
    12841 tgaagtactg caacaccgtc agaccttcac ggaagttcgc cgtgatgggc gtctcgatga
    12901 tttcgccaga aggctttgcc atgaggccac gcataccggc cagctgacga atctgctggt
    12961 tggaaccgcg agcgcccgaa tcggccatca tgaagatcga gttgaagctc ggctgggtgg
    13021 tcgtctcgcc ggtttgggga tcggtgacct gatcgtttcc gatggactcc atgaggtcgc
    13081 gagcgatctg atcggaagcc tccgcccaaa tgtcgacgac cttgttgtag cgctcgccgt
    13141 cagtgatcag accttcctga tactgctcga cgaccgtggt gacgtcctct tgggccgccg
    13201 ccaaaacgtc cttcttcgaa tcaggaatga tcatgtcatc catgcagatc gagataccgg
    13261 cacgggtcga catctcgaaa cccatcgtgc gcaatcggtc ggcgagaagg acggtgttct
    13321 tgttccggct ggctcggtaa cactcgtcga tcagcgcgct gagtgacttc ttgtcgagca
    13381 ccttgttgac cgacgggaac tccattcccg gaggcaaaac gtcgctcacg agcacgcggc
    13441 cgacggtggt ggtgtaaatc tcccccttgt atcggtatcg aatgcgagag tggagaccga
    13501 ccatgttctg gtcgtacgcc atgcgaacct cgtcggacga ggcaaagatg ccggtgaatt
    13561 ggccttcctt ctcctgcccc tcgcggtacg cgcccttacc gaactgcttc tcgcgcgtca
    13621 tgtagtagag gccgagaacg atgtcctggg tcgggttgat gatggggcgg ccgctcgcgg
    13681 gcgacaggat gttgttggtg ctcatcatga gcacgcgggt ttccatctga gcctcgacgg
    13741 agagcggcac gtggaccgcc atctggtcac catcgaagtc cgcgttgaag gccgtgcaaa
    13801 ccagcgggtg gaggcgaatc gccttgccct cgatcagcac gggctcgaat gcctgaatgc
    13861 ccagtcggtg caaggtcggc gcgcggttga gcatcaccgg atgctcggtg atgacctctt
    13921 cgagaatgtc ccaaacctcg ggcttctcct tctcgacgag cttcttcgcg ctcttgatcg
    13981 tggtgacata accacgctct tcgagcttgt tgtagatgaa tggcttgaac agctcgagcg
    14041 ccatcttctt gggaagcccg cactggtgca aacgcagtgc aggaccgacg acgatgaccg
    14101 aacggccgga gtagtcgaca cgcttgccga gcaggttctg ccggaagcgc ccctgcttac
    14161 ccttgagcat atcggagagt gacttgagcg ggcgcttgtt cgggcccgtg atggtcttgc
    14221 cacgacggcc gttgtcgaat agcgcgtcga ccgcctcctg cagcattcgc ctctcgttcc
    14281 ggatgatgat ctccggagcg ttgagctcga tgagccgctt gagacggttg ttgcggttga
    14341 tgacgcggcg gtacagatcg ttgagatcgg aggtcgcgaa gcggccgcca tcgagcggca
    14401 ccaagggacg cagatcgggc ggaagcaccg gaatcacgga aagcatcatc cactccgggc
    14461 ggttacccga atcgcggaag gcctcgatga ctttaaggcg cttggcgagc ttcttgcgct
    14521 tcgcctcgct cgtggcctcc ttgaggtcca agcggagttg ttccgccatg gcgtgaacgt
    14581 cgagatcacg gagcatctcg aggacggcct cgcctcccat gccagcatcg aacgcgtcgt
    14641 cgccatactc gtcgagcatg tcgtggtagc gatcctcggt gaggatctca ccacgctcga
    14701 gggctgtttc tttgggattg agcacgacgt agctctcgca gtagagaacg cgctcgagat
    14761 ctttgagcgt gatatcgagg atggcgccaa tgcggctagg caagctcttg aggaaccaaa
    14821 tgtgggctac gggcgttgcg agggtgatgt gaccaagccg ctcgcggcgg accttgctct
    14881 ggatgacctc gacgccacac ttctcgcaga cgatgccccg gtgcttcatg cgcttgtact
    14941 tgccgcagat gcactcgtag tcttttaccg gtccgaagat cttcgcgcag aagaggccgt
    15001 cgcgctccgg tttgaacgtg cggtagttga tggtctcggg ctttttgacc tcgccatgcg
    15061 accactcgcg gatcttctcg ggcgacgcca gagaaatacg aatcgctgaa aaggccaggg
    15121 ggtctttcgg cttttcgaag aagctgaaga tatccttcat gacttactct tcctccgcag
    15181 caagcacgtc atccgacctg cgctccaatt cactcgtctc gaccagctcg atgttcaggc
    15241 agagcgcctg aagctccttc atgaggacgt tgaagctctc cggcagcccg gcttcgagcg
    15301 agtagtcccc cttcacaatc gactcgtaca ttcgtgttcg accttgcacg tcgtcggact
    15361 tgacggtgag gaactcctgc aacgcgtacg ccgcgccgta ggcctccatc gcccagactt
    15421 ccatctcgcc gagcctctgc ccgccgaact gtgccttgcc accgagcggt tgctgtgtga
    15481 ccagcgagta aggcccgatg cttcgggcgt gaatcttgtc gtcgaccaaa tggtgcagct
    15541 tgagcatgta catgatgccc acggtcacgt cctcgtggaa cgcctcaccg ctacgcccat
    15601 cgaagagaac cgtttggccg ctttcctgca caccagcgag cttcagaagc tccttgatct
    15661 ctttctcgct tgcaccatcg aacaccggcg tggccaactg cagaccgtgc ttccagctct
    15721 tggccatctt ggcgacctca ccgtccggta gggtgtcgag caccttgacg atgtcgccgc
    15781 ccttgaagat cgaacgaatc cgctcgcgaa gctccgcagc gctggccttc tgttcgacca
    15841 tctcctgaag ctgagtgccg agcagatagc cggcccagcc gagatgtgtc tcgagaatct
    15901 ggccgacgtt cattcggctt ggaacaccca gtgggtttag aacgatgtcg acgggcttgc
    15961 cgttcggtag atacggcatg tcctcttccg gcaggatccg gctgacgaca cccttgttac
    16021 cgtggcgccc cgccatcttg tcgccgacct ggagcttacg cttgatggcg acgtagacct
    16081 tgaccatctt gatgacgccc ggagggagct cgtcgccctt gccgagcttg tcgatcttct
    16141 cctggaacag catcttcacg gcgtcgacct ggtcatcgac ttgctcgaga atcgcgtcgg
    16201 tcttgtcttt gtccttgtcg atctcgatgt ggccccagta cttgtggggg attccgtcga
    16261 ggtctccgtc ggtgatcttc tgacccttgg tgaggagctc tgctcccttg tcacccacca
    16321 gcttcgctgt ggcggtgctc cctacgaagt acttgcgaac gcgatcatgc gcggagtcga
    16381 gaacgatctt gatctcgtcc gccatgtcct tttcgagctt ggctcgctcg gactcctcga
    16441 tctgcagcga gcgctcgtcc ttttcggttc cctttcgggc gaatacgcga gcgtcgatga
    16501 cgatgccggt cacccctggc ggaaccttga gtgacgtgtc gcgcacgtcg ccggccttct
    16561 caccgaagat cgcacgaagg agcttctcct ccggagagag ctgtgtttcg cccttgggcg
    16621 tgatcttgcc gactagaatg tcgcccgagc tcacctctgc gccaatccga acgataccgg
    16681 actcgtcgag gtccttgagc gcttcttctc cgacgtttgg aatgtctcgg gtgatctctt
    16741 ccttgccgag cttggtgtcg cgagcgacgc actcgaactc ctcgatgtgg accgaggtgt
    16801 agacgtcgtc tttggcaatc cgctcggaaa gcaagatcga gtcttcgaag ttgtacccac
    16861 cccagggcat gaacgcgacg atcacgttct gtccgagtgc aagctctccg cggtccgtgc
    16921 tggggccgtc tgcgatgacg tcaccggcct tgacggcgtc ccccgggcga acgatcggcg
    16981 tctggttgta cgcggtgttc tggttcgaac gctgaaactt gttcagccgg tagatgtccg
    17041 ggaacgcgcc cgcgagacct tcggctttta cgacgatacg agtcgcgtcg actgattcga
    17101 cgacaccgtc ctgttgcgca acgacacata cgccggagtc tctggcgacc cggcgttcga
    17161 tgccggtccc ggcgatcgga gcgtgcgttc gcacacaggg aacggcctga cgctgcatgt
    17221 tcgagcccat caaggcacgg ttcgcatcgt cgtgctcgag gaatggaatc aacgaagcag
    17281 cgacggacac gagctgattg ggagagacgt ccatcaactg aacgctgctc ggcggcacca
    17341 tcacgaattc accattgaaa cgagcgctga tcagctgacc ctcgagctta cggctgacgt
    17401 gcgcgatctt cgcgttcgcc tgcgcgatgt agtgcccctc ttcctcaagc gcagagtacc
    17461 aacgcacgtg gtcgtcgagc acgatgccgt cctccacctt tcggtagggg gtctcgacga
    17521 agccatattc gttgacccgg gcataggtcg agagcgacgc gatcagaccg atgttcggac
    17581 cttctggcgt ctcgatcgga cagatacggc cgtagtgagt cgtgtgaacg tcgcgcacct
    17641 cgaagcctgc acgctcacga gtcaaaccgc cgggcccgag cgccgagaga cgccgcttgt
    17701 gcgtgacctc gctgagcggg ttggtttggt ccatgaattg agagagctgg ctcgagccga
    17761 agtactcctt gacgaccgcg ctgaccggct tcgcattgat cagatcgtgg ggcatgagcg
    17821 tctcaatctc ttgagacatg ttcatgcgct ctttgatggc gcgctccatg cgtacgagac
    17881 cgatacggta ctgattttcc atcagctcgc cgacggcgcg gacacggcgg ttgcccaagt
    17941 ggtcgatgtc gtcgaccgaa ccacgaccgt tcttcagctc gatgagattc ttcaccgtat
    18001 cgaggatgtc ggtgttcgtg agaacggtgt ggtcgagagc cggtcgctcc tccggctcca
    18061 cgtccttgta gaacttgtag ttcaacttga gacgcccgac ctggctgaga tcgtagcgct
    18121 ccggattgaa aaacaagttg ttgaagagat tgcgcgcggt gtccagcgtg ggtggatcgc
    18181 ccgggcggag ccggcgatag atctcgagga tgccttcgtc ctgcgactgg accttgtcga
    18241 cgagcaaggt gtcccggaga taagacccga cgttgagacc gtcgatgaaa agcaccttga
    18301 actcgttgac accggcctcg cggagcgcgt cgatgcgagc ctcggtgacc tcctcgttca
    18361 cagcgagcaa tatctcgccc gtttcctcgt cgacgacatc ctctgcagcg actttgccaa
    18421 tcacctcggt gaagtcgatc ggcagatagt cgagaccagc ggacttcagc ttgcggatcg
    18481 ctgcgcgcgt gaacttgcgg ttctttcgga cgatgacgtc gtcaccgacc gagatgtcgt
    18541 gcgtcgcacg ttgaccgggt agaaggtcgt actcgatcga tttggcgtac tgatccttcg
    18601 agaggatgta aatcgtctcg gtgtcgtagt agtagttgag cagctgctgg gtgttgtacc
    18661 cgagtgcctt cagaagcacc gtcgcaggca tcttccggcg gcggtcgatg cgaacgtaga
    18721 gcaagtcctt cgggtcgaac tcgaaatcga gccatgagcc gcggtaagga atgatgcgcg
    18781 cctggtacag caacttgccg gacgaatgcg tcttgccacg gtcgtgatcg aagaagacgc
    18841 ccggacttcg gtgaagctga ctgacgacga ctctctccgt gccgttgatg atgaacgttc
    18901 cgttcaccgt catgaggggg atctcgccga agtagacttc ctgctctttg atgtcgcgaa
    18961 cggctcgctc ggtgctgtcg ccgccagcgt cataaatgat gagctggatc gtgactttga
    19021 tgggcgcggc gtaggtcatg ccacgctgcc gacactcatc gacgtcgtac tttggccgtt
    19081 cgagcgtgta accgctgaac accaactcgc tcgttccatt gaagtcgcga atcggaaaca
    19141 cgctgttgaa gacgccctga agaccggtgt tctcccgatc gtctctagaa acgtccgttt
    19201 gaaggaactt atcgtacgag cgcttttgaa tgtcgatcag gttcgggatt tcgaggatgt
    19261 gctcgatctt cccaaaggaa tgtcgcattc gaaagttcgt ctgaatcgtc gccgccatga
    19321 gagagactcc ctaccgaacc actgctagtt ggaaccctaa agacaagaat gaagagacaa
    19381 gtgcgcgaca aggagacctg tcggcccccc gccgctgcgc ggaaagacgt gaatgctctg
    19441 ctgcgcgtac cggtgctgcc ccggccggga ccttaacccg gtcgggcccg tgtgccaagt
    19501 cgcggcaccg ggctccaaaa ggtaacccca tagggttact tgacggtgac cttggcgcca
    19561 gcttcttcga gcttcttttt gatgtcggcg gcctcttcct tggaaacggc ctccttgacc
    19621 actttgggag cagcctccac cagggccttg gcatctgcca gaccaagctg ggtgatctca
    19681 cggatcgctt tgatcacgtt gatcttcttg ccgcccgcct cggtgagctc aacatcgaac
    19741 tcggtctgct cctcgaccgc ctcagctgcg ccgccacccg cgggacccgc gactgcaacc
    19801 gctgccggtg ctgctgaaac gccccaagtc tcctcgagct gtttgaccag gttcgcaact
    19861 tccatgacgg tccaggagct gagctcttcg accatctgtt ccagattcat atttgccatt
    19921 cttgactctc cgctaaccgt gcgtgcgaca cggttaaagt tgtgtttggg ttactcgccc
    19981 gccgcttgac gacgcttcgc atcgagtacg agagcgaggt tttggcccgg tgcgttgagc
    20041 tgcccgacca gtttctgcat gggcgccatg agctgcgcga gcagcgaggc gcggatttcg
    20101 tctttgcccg gaagcgtagc gagctcgttc tcgacccgct tcgcgctcag aacctggcct
    20161 tcgatgaggc cacacttgac gaggagcttc tcttttccgt ccttcggctg aaggatgtcg
    20221 gtaaactcct tgcggaagct cttgatgact ttggcagcgg ccgaagggtc ttcgaagctc
    20281 caggcgatcg cagtcgggcc ggcgagatag gcggtgaact cgtcgttctc ggcgagtgcc
    20341 gacccttcga gcgcctttcg gacgacgttg ttcttgacga ctttgtactc gactcccgcc
    20401 tcgcggaagc gcgagcggag gttcgtgatc agctcgacgt tgacgccccg aaagtcgacg
    20461 agcacggttg cgctcgcggt gtcgaagctc tcacggaccg aagcggcctg ggcggttttt
    20521 tcttgtcgtg taaggctcat gattacgcct ccgcctctat agtgccgggg tcaaccttga
    20581 tgccggggcc catcgtggag ctcaatgtga tgctgcgaag gtagacgcct tttgctgcgg
    20641 ctggcttggc cttcacgagc gcgcccacca aggacctgac gttcccggcg agctcgcctt
    20701 ctccgaagga ccgcttgcca acctgcgcat gcacgattcc ggccttttcg gcgcgaaact
    20761 cgaccttgcc ggccttcgca tcgctcactg ccttggccac gtcgaaagtg accgtgccaa
    20821 ccttgggatt cggcatgagg ccgcgagggc cgaggacgcg accaaggcgg ccaacctgtc
    20881 ccatcatgtc gggagttgcg atgacggtgt cgaaatcgag ccagccttcg ttgatcttgg
    20941 caaccatgtc gtcgccaccc atgaagtctg cacccgctgc ctcagcctcg ctcaccttct
    21001 cgcccttggc gaaaacgaga actcggttgg tcttgcccgt gccatgcggc aggacgacgg
    21061 cgccgcgcac catctgatcc gcgtgctttg gatccacgcc gagtcgcact gcaacgtcga
    21121 cagactcatc gaagttggcg aacgccaatt ccttcacgag tgagacggct tcctgcaatg
    21181 cgtagcggcg gcttttgtcg ggcttctgta aagcacccgc gcgctttttt ccgggtttca
    21241 ttctgactcc ttgcgacagc tcgaagccgc ctaccaccta ctggggccct gggtggtcca
    21301 gggaagcgaa accaatcgct cgagagaact agtcgactag ctcgaccccc atggaacgcg
    21361 cggtgccgat gatgctctgc atgcatgtct cgagcgaacc tgcgttggtg tcttggatct
    21421 tcaccttggc gatctccatc acttgatccc gcgtgatctt gccgactttg tccttgttcg
    21481 gacgcgccga gccagattcc agattcaagg ccttcttgat caagacggcc gcgggcggcg
    21541 tcttcatgat gaagctgaag gagcgatcct gataaacgga gatcacgacc gggatgatca
    21601 gaccggcatc cttctgggtc ttcgcgttga actccttaca gaacgccatg atgttgacgc
    21661 cgtgctggcc aagcgcaggt ccaaccggcg gagaagggtt tgccttccct gcggggagct
    21721 gaagtttgat ctgtccgatg acctttttca tgggtgcggg cttctccgga gcggcgagtc
    21781 atgcctcacg gcacgaaaaa tgcaacccaa tttctgtgaa aactggatcg ctttcgacac
    21841 atccctgtct ggggatggtt tgatcggcta gttagttggt cttttcgacc tgggcgtagt
    21901 ccagctcgac aggggtcgcg cggccgaaaa tcgacacgag cacccgaacc ttgtgcttct
    21961 cgtggttcac ttcctcgatc gcaccggtga aattggcgaa ggcgccgtcg gtcacgcgaa
    22021 catggtcgcc cttctcgaac tggacgcggg gctttggctt ggcagcgccc tcctcgacct
    22081 gttgctcgat cgcagcgatc tccggttcgg gaaccggcga cgggtgccgg ccacccacga
    22141 agccgttgac cttgggcgtg tccttgatga gatgccaggt ctgatcatcg aggtccatct
    22201 ggacgaagat gtagcccgga tagaaggtcc tcgagctgag tcggcgcttc ccaccgcggc
    22261 tctcctgcac attctctttg gggatgagaa tgttcccgaa gcggccttcc atacctgcct
    22321 gcttgatgcg ctcttcgagg gagagcttca ctttgttttc atagcccgaa taggcctgga
    22381 cgacatacca cttcatggaa ctcacacgtt gtaaatgagg tccgtgatgt gggaccaaac
    22441 cgcgtcataa acgccgagaa tgattgctgc gatcacagag acgatgagca ccacgatcgt
    22501 ctgcgaccag gtttcttttc gggttggcca gctcactttc gagagctcga cgcaaacctc
    22561 gacgacgaag gtgtgaacct tcttcgaacg ataggcgacg accgcgctga tgaaggccac
    22621 gaggccggcc ccaaatgcaa tcgcagtggc gttggcctcc gggattgcgt ccacgctgtc
    22681 ggccagcatc gtccagatcg cgttgctgca cttgattagg aaccaggcga gcgtgatggc
    22741 acaggcggcg taggcgaatt gaacccagcg ctctacgccg agcgttccgg tcggtctcgc
    22801 tttgtcttgt tcgttcgtga tcgccatcgt tgttcctcgc ggcaggccag gagggattcg
    22861 aacccacaac ccgcggattt ggaatccgct gctctaccaa ttggagctac tggcctgggt
    22921 actcttctta cttggtctct ttgtggtccg ttcgccggtt gcatttggga cagaacttct
    22981 tgagcgtcag tctctcctgc tggccggcct ttctcaactt tgtcgtctgg tagtttcgcg
    23041 tcccgcactc gtcgcacgcg agggcgattt taactcgcct atcactcaat gatcttggag
    23101 atgacaccgg caccgatggt tcggccgcct tcgcgaatcg cgaagcgctg cttctcttcc
    23161 atggctgctg gcgtgatgag gttgaccttc atcttcacgt tgtcgcccgg catgaccatc
    23221 ttcacgtctt cgggaagctc g
//
LOCUS       DASRZF010000115         2533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3173144, whole genome shotgun sequence.
ACCESSION   DASRZF010000115 DASRZF010000000
VERSION     DASRZF010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3173144"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..513
                     /locus_tag="VFG22_05380"
     CDS             166..513
                     /locus_tag="VFG22_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415705.1"
                     /translation="MVGQVAGALGETADPSKGLVAVIYDEDVAVGGETADLGVNYGFA
                     FVFDAGDGNPVEGNMLLAGGGSEVIFANVDVSANVMPSATDASDQDCAFEFPDAVYPS
                     QAKVLTEVAMTCP"
     gene            649..2319
                     /locus_tag="VFG22_05385"
     CDS             649..2319
                     /locus_tag="VFG22_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415706.1"
                     /translation="MHGLRLFAALSSALLITFGCVSSPNVTPSIPKPSEPTAPLLEGM
                     GDLHWAITTDSELAQRYFDQALTLAYGFNHLEAERSFREAARLDDDCAICFWGAALVL
                     GPNINDPVPTPERELEAYQTLEEAKARLPRASEKERALIEALGARYAASPPSERTALD
                     HAYADAMRTVARRFPDDLQIQTLFAEAMMDTMPWAYWTEDGEPKPLTEEVFATLDFVL
                     ENDPDHPGANHFYIHAVEQEQPERGVAPADRLRRVVPGAGHLVHMPSHIYIRVGRYHD
                     GSLANELAVTADNDYVTQCRAQGIYPVAYVPHNHHFLWATTTMEGRSADAIAAARHTA
                     AHVDRSLMREPELAMLQHFSAIPYYALVRFGKWDEILREPAPDADLLYPTAVWHYARG
                     IAYAREGKLKQARAELDRVRAIAVDPRLQPLRIWGLNPMSSLAEIAGAVLHAEIAAAR
                     NDTKTAVAELRKAIAIEDGLIYGEPPDWFYPVRHNLGAVLLEAGRARAAEKVYREDLA
                     RFPDNGWSLFGLAQSLRVQGKAQEAAEVLGRFETAWQYADVEIRASRF"
     gene            complement(2332..>2533)
                     /locus_tag="VFG22_05390"
     CDS             complement(2332..>2533)
                     /locus_tag="VFG22_05390"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HET6415707.1"
                     /translation="GPSPTRLEHGSLIVFAVGPEGGWSDEERAMLEARGARSLRIGGR
                     VLRAETAVFAGLTLLQHAYGDM"
BASE COUNT          469 a          810 c          805 g          449 t
ORIGIN      
        1 gtcgcgaccg tcggccccgt cctctgacga tttccgcggc cgcgcaagct ccgagaatcg
       61 gccgcgcgag ccgcgaatcg cgttctgagg accaaatttg cagaaggccg gcgtcgcccc
      121 gcgggcggcg ggagtggggt agagctcgag gtccttcccg atgctttggt cggtcaggtc
      181 gccggtgcct tgggggaaac cgccgatccc tccaaagggc tcgtcgcggt catctacgat
      241 gaggacgtcg ccgtcggcgg cgagaccgca gacctcggag tcaactacgg cttcgcgttc
      301 gtcttcgacg cgggcgatgg aaacccggtg gagggcaaca tgctcctcgc cggcggcgga
      361 agcgaagtca tcttcgcaaa cgtcgacgtg agcgccaacg tcatgccgag cgccaccgac
      421 gcgagcgatc aagactgcgc gttcgagttt ccagatgccg tctacccgag tcaggcaaag
      481 gtgctcaccg aggtcgcgat gacctgtcca taaacgcggt aagcaaagca tgaggtgaca
      541 agcgggctgg cgaatgagtc gtcagcccgc ttcttctttg tcagtccgat gcggccgggg
      601 gtgacggggg tatcgcatct tggtaaaaag gacctcgagg aggttgccat gcatggactg
      661 cgtctgttcg cagcgctttc ttccgctctg ctgatcacct tcggttgcgt gtcgtccccg
      721 aacgtcacgc catcgatccc aaagccgtcg gagccgacgg ccccgctcct cgaaggtatg
      781 ggggatttgc attgggcgat cacgaccgat tcggagctcg cgcagcgcta cttcgaccaa
      841 gccctgacgc ttgcgtacgg gttcaatcac ttggaagccg aacgctcgtt tcgcgaagca
      901 gcccgactcg atgacgactg tgccatctgt ttctggggcg cggcgctggt gctcggaccc
      961 aacatcaacg accccgtgcc gacgcctgag cgagagttag aggcctacca gactttggaa
     1021 gaggccaaag cgcggttgcc tcgggcgagc gaaaaggaac gcgctttgat cgaggcgctt
     1081 ggcgcccgct acgcggcatc gccaccgtcg gagcgcaccg cgctcgacca tgcgtatgcc
     1141 gacgcgatga gaacggtcgc ccgacggttt cctgacgacc tccagattca aacgcttttc
     1201 gccgaagcga tgatggatac gatgccttgg gcgtactgga ccgaggacgg ggaacccaag
     1261 ccgctcaccg aagaggtctt tgcgacgctc gatttcgtac tggagaacga ccccgatcat
     1321 cccggcgcga atcatttcta cattcacgct gtcgagcaag agcagccaga gcgcggagtc
     1381 gctcccgccg acagattgcg gcgtgtggtt cccggcgccg gccacttggt acacatgccc
     1441 tcgcatatct acattcgggt cgggcgctac cacgatggct ctcttgcgaa cgagctcgct
     1501 gtcacagccg acaacgacta cgtcacacaa tgccgcgcac agggcatcta cccggtggct
     1561 tacgtgccgc ataaccatca cttcctttgg gcgaccacga cgatggaggg gcgtagcgcg
     1621 gacgcgatcg ctgccgcacg tcacaccgct gcgcacgtcg accgctcatt gatgcgcgaa
     1681 cctgagctgg cgatgcttca gcacttctcg gcgattccct actacgctct ggtgcggttc
     1741 gggaagtggg acgagattct gcgtgagccc gctcccgacg cggacctgct ctatcccacg
     1801 gcggtctggc actacgcgcg tggcatcgcg tacgcgcgcg aggggaaact caaacaagcc
     1861 agggcagagc tcgaccgcgt gcgcgcgatc gcggtcgatc cacgcctcca gccgttgcgg
     1921 atctggggcc tcaatccgat gagctcgttg gcggagatcg cgggggcagt gctccatgcg
     1981 gagattgccg cggcacgcaa cgacaccaag acagccgtcg ccgagctccg aaaagcgatc
     2041 gcgatcgaag acggcttgat ctacggcgag ccacccgatt ggttctaccc ggttcgccac
     2101 aacctcgggg ccgttctgct cgaggcaggc cgtgcacgcg ccgcagaaaa ggtctatcga
     2161 gaggaccttg ctcgattccc ggacaacggc tggtcgctat tcgggctggc ccagagtctt
     2221 cgtgtacaag gcaaggcgca agaagccgcg gaggtgctgg gtcgcttcga gacggcttgg
     2281 cagtacgctg acgtcgagat cagggcctcg cgcttctagg tcatgcccgg tctacatatc
     2341 tccgtaggcg tgctggagga gcgtcagacc ggcgaacaca gcagtctccg ctcgcaaaac
     2401 gcgtcctcca attcgaaggg accgagcccc acgcgcctcg agcatggctc gctcctcgtc
     2461 gctccagcct ccttcgggac cgacggcgaa gacgatgagc gagccatgct cgaggcgcgt
     2521 ggggctcggt ccc
//
LOCUS       DASRZF010000116        22896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2486329, whole genome shotgun sequence.
ACCESSION   DASRZF010000116 DASRZF010000000
VERSION     DASRZF010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 22896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22896
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2486329"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="VFG22_05395"
     CDS             complement(<1..712)
                     /locus_tag="VFG22_05395"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET6415708.1"
                     /translation="MFDDYVHYDGLGLAELIARGEVQPKEVLEAAILRAEKLNPALNA
                     IVTPLYEHAHRRLREDLSGPFRGVPFLLKDAHHALQGTPMSNGSRLHKGEISEHTAEI
                     VRRFLRAGVVVFGKTNTPEYKLSVFTMPKAWGPTRNPWDVTRSAGGSSGGSAAAVAAG
                     IVPMASATDEGGSIRMHAAACGVFGLKPSRGRNPIGPDFSWEYEGQSTSHVISRTVRD
                     SAAMLDATAGTEPGSPYVA"
     gene            complement(719..1504)
                     /locus_tag="VFG22_05400"
     CDS             complement(719..1504)
                     /locus_tag="VFG22_05400"
                     /inference="COORDINATES: protein motif:HMM:NF018949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HET6415709.1"
                     /translation="MEEGWITALLWLLFGGTHIGLTMRPVRERLVRRLGEGGFVFVYS
                     VIAIITFAALVHYVAEHRVAEPQAPLLVTIPPVRGALIALSVAGFSLFITAVLVYPRL
                     PMATFRHRVVPARGVQQVTRHPFFSGIAIWAAAHALLAPSPAPFVFFIGVVVLSLVGG
                     LHQDRRLFQELGEPYRSYAASTSFWPLVALVTKRQKIQWREQPWFAYGLGIGTSLALY
                     QVHADILSHGGAYVIGFVSVGSIIAILNSRARRARPHSDAGEG"
     gene            complement(1708..2676)
                     /locus_tag="VFG22_05405"
     CDS             complement(1708..2676)
                     /locus_tag="VFG22_05405"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase family protein"
                     /protein_id="HET6415710.1"
                     /translation="MGKGSKAWLEAFTSEEIRALRVIRAHRAWLTVFVNYGLIAASMT
                     MVALVPNVLTVALALFVIGTRQLGFAVVMHESAHRTLFKNRKLNDFVGNWLCGYPIYL
                     SADMYRTHHLEHHAKTWTEGDPDLALATAFPVSRRSMARKVMRDLFGVTGLKQLIGTS
                     YLIVKVLRGEQVDAGTLPLRLERGPAIRMVVGTLVTNLILFAFLWSIGQPLLYLLWLG
                     AWLTTNKLVARIRSIAEHAVVPDPSDPIGQTRTVLAGPFERLFIAPNRVQFHLEHHLL
                     MTVPHYNLPKFHTMLRERGLLEGACVAPGYAQVLSLALRPAAQSPV"
     gene            complement(2680..3111)
                     /locus_tag="VFG22_05410"
     CDS             complement(2680..3111)
                     /locus_tag="VFG22_05410"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET6415711.1"
                     /translation="MITVAQLLERKGHNVEAIAPDATVFEAIKRMSDLDIGALVVREG
                     GKVIGLLTERDYARNVFLRGKASPTTPVRDAMDVEVLSVTRDKTTRDCMAIMTGKRTR
                     HLVVMEDGELLGILSIGDLVRSIVAEQDMTIEQLEQYILRG"
     gene            complement(3215..3859)
                     /locus_tag="VFG22_05415"
     CDS             complement(3215..3859)
                     /locus_tag="VFG22_05415"
                     /inference="COORDINATES: protein motif:HMM:NF019559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HET6415712.1"
                     /translation="MTLNGVGELMTALGLAVLLVGERKDRRALRFLGKPFASFGFLVA
                     AVGFGALESRYGKTVLAGLVLGAIGDVCLLGRARQYFVAGLTSFLLGHVAYVFAFGLL
                     PFNSSYALVAGLAMGLLMFFVARWVFPHAVEMRVPIGIYMLVIAGMCVVAVGAGGAGA
                     PWMVPVGALMFTASDIAVVRDRFVKRGFVNRLWGLPLYYAAQLIIAWSIAVVNR"
     gene            complement(3856..5508)
                     /locus_tag="VFG22_05420"
     CDS             complement(3856..5508)
                     /locus_tag="VFG22_05420"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET6415713.1"
                     /translation="MNFKDAADVAKRWADEHRELMIIGVALPLSTLASGWDRVRNAFA
                     KPVRPEGHDARVERVQAEVRRYAKARRQGDAWAQRPLRTDRKGAASLNTRVADKSGSE
                     TIQMNDLTSVLEVDENAGIVRLEPFATVGDVAQHLDEKGLQLEATIEMKDATLGGLVM
                     ALGMTTHSHVCGLVHDTVTAYEIVTAEGDLVRATADNEHAELFRALPWSHGTLGLLVA
                     IEMRVIPAPSHVRLVYRPFHSLEAYAAEYRRLASDADPPHFIETIIFGKDRAVVMEGH
                     LASPEEVRAGSLPINPLSRYYKPLFYKHVESMLELEEGERCEELVPMYDYLMRHDRSM
                     CMCLAQILPTANEPWFRYTMGWMLPPNTTFLKGTRPQAERENTIRKQVWQEYAFPAEY
                     FEEMVEYVHREFEIYPLLAYPCRVIDHGGLVRRPANRGKPYSGHAETETFLDIGIYGF
                     PRSIKEGEERFPAVTKVRRLEQKARSLGGFLHTYCDVFSTEAEFEEMFDHGLWREMRA
                     KYNADGTFPTIYEKVKPELDPLQFLEEEESWLGLSEAERTVS"
     gene            5614..6816
                     /locus_tag="VFG22_05425"
     CDS             5614..6816
                     /locus_tag="VFG22_05425"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HET6415714.1"
                     /translation="MSDEKPLPLAGVRVIEFTHMVMGPAAGLMLADMGADVIKLEPVH
                     GDKTRSLPGSGAGYFPAYNRNKRSLCIDLKSDEGKAAVLRLVDTADVIVENFRPGTMD
                     RLGFGYEELRKRNKGLIYCSEKGFLAGPYEERTAMDEVAQMMGGLAYMTGPPGRPLRA
                     GASVIDVQGGMFGAMGVLAALLQRNLTGEGQKVISSLFESTVFLVGQHMAQYAVTGEA
                     AAPMPARISAWAIYQIFATKDDDLVFVGIVSDKQWELLCGAFGLDEMAADATLKTNAG
                     RVAEKDRLLPMISDVFASYSKAELMAKLESRGLPFAAITRPEELFDDPHLNAGAGLLP
                     FTVTDGDRKGNEAKLPALPLEMDGKRFGVRRPVPRAGEHSREVLIEAGYPEEAIDSML
                     AAGIVTAE"
     gene            6894..8615
                     /locus_tag="VFG22_05430"
     CDS             6894..8615
                     /locus_tag="VFG22_05430"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET6415715.1"
                     /translation="MSEQLSLEQAPAGLGAALRNAGFESFTAIQHAILKPELEDRDLR
                     ISSQTGSGKTVALGLLLAPSVANAATTARPQKSRKSAPASPVALLIAPTRELAGQLGR
                     ELSWLYKPLAARVTVVTGGTSVMQERSELYSLPQVVIGTPGRLLDHLRAGALNLDQLA
                     TLALDEADEMLDMNFEEELDGILGFAPDARRTHLVSATFPREVVRLADRLQKDPVSVQ
                     GTTLGKANADIEHVVMHVRQADRFNAAINVLLRYPEDKTLLFVRTRTETVRLADELCA
                     AGLAARALNGEMTQRERTATFTQFRNGTIRYLVATDVAARGLDVQDIGRVIQVDLPDN
                     ADVFTHRSGRTGRAGRKGTNVLFAASRATARVRNTLRAAGVEARILPIPTPEEIFAAA
                     DERIGESFLQDLESQSDKTNLRARALAERLLAKHSPAELVACLLQREAERSMGTPKQV
                     RDDSQPAHREARPRKTGPRSRSRQRPQGDFITYQVSWGEQAGADTRRLLAMVCRRGGV
                     SSEVVGSIRVASNSSTVDIVASEAQRFERAVARPDKRNPRAKFRRWDSSASAKRPRNR
                     SADARCA"
     gene            8689..9456
                     /locus_tag="VFG22_05435"
     CDS             8689..9456
                     /locus_tag="VFG22_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415716.1"
                     /translation="MRRVLFFATLLFLVLGCKDREETSAVPTANEALAATAEEGSGVP
                     EVGNDFDLGDEAEARPDGEDAEKASVESADNAAPPVRDLGAELRAALGSPVDCLNDYQ
                     PSPGTVVRVSVSAIVRQSGVVIEPSANGAGLSANDRRCIEQRAGDVVLAPFEAETSRP
                     VSTYVDLVVGGGPVEETDVGLPDPKLKDVRGIEGGIVDTITPSGIPVEKLPADAPVGP
                     RGDPVEGPKGVPITGPSPKPIEGYQLDEKEGAERWTE"
     gene            complement(9485..10018)
                     /locus_tag="VFG22_05440"
     CDS             complement(9485..10018)
                     /locus_tag="VFG22_05440"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HET6415717.1"
                     /translation="MHNTGKTVLLIATFVFVGCAGQVKTNISYDDTVDFASYKTFAQT
                     PPPSYADNMPGYSEITGRNIQDRISMVLQQKGLEPAGMDEADLQVSFTLGGQQRQEAE
                     YFTGWGWYGPGEFQTENYVEGSLVIQMGDRVKKRLVWHGYGTEDIFSEAGNDEIIMKA
                     VDAVLAKYPPAQGGAAK"
     gene            complement(10128..10451)
                     /locus_tag="VFG22_05445"
     CDS             complement(10128..10451)
                     /locus_tag="VFG22_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415718.1"
                     /translation="MQASAIIPVVAIVAIASAGCGRNDSNCGPSCSVISSCTDRIYQS
                     CMEECEGDFNEASDASIACGTAVDDRAACVAGLSCAELDAWTQKVPPEDYPCRDEDLA
                     VDDEC"
     gene            10564..12474
                     /locus_tag="VFG22_05450"
     CDS             10564..12474
                     /locus_tag="VFG22_05450"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HET6415719.1"
                     /translation="MTAPRRVRLPEHTVEIVSDAGEGAQKAAIAFAQVCAKAGNGLWT
                     VEIIPSEIRPPPHTTGSASGNRIRLAERDRVTNAGNEANVVLAFNEMALLSRLEAGSL
                     ADDVLVLIDDQWATHEVEKYQTSYREVLDTFRARGATIIEVPMERETLRHAEDAQQGK
                     NMFAVGLLCALYSKDLDVLDDVIRVIFKSKAPAVIELAVRLAAAGYQYGVTNFKECFE
                     IEAHPTDVPKVAMNGNTALALGAISAGFELCSMYPITPATSASHALSETFEDFGGFVH
                     QAEDEIAAIGVALGASYAGKTALTITSGPGMALKTEFQGLAVMTETPLVIVDVQRGGP
                     STGLPTKIEQSDLLHALYGAPGDAPKVVIAPSTIEECYYILAVALKIAEEFRMLVIVL
                     SDANLATGQQLFDRPDVSKTPRREPLDLSPVPPGALPYDWDPTTGLSERRIPGQPGGM
                     GVTTSLNHDRNGKACYDAERNQRGHDMRSRKLAALQQSLQPPSVYGAPEGDLLLVGWG
                     STRGAIEEAVDAARGEGLAVSSIHIQFLSPLPPGLKDIFANFRKVITVELNYSDDLND
                     PLITSENRRYGQLAWILRAATLHDIDCFAKVPGRPYMPVEILGEVKRILGEQTEVEDP
                     SERFVASEGANR"
     gene            12471..13430
                     /locus_tag="VFG22_05455"
     CDS             12471..13430
                     /locus_tag="VFG22_05455"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET6415720.1"
                     /translation="MISMLAEESLLEICAIETHSLDEYESEQPRWCKGCGDHGVLASL
                     QRVLRKNNLEPENVVSVSGIGCSSRLPHYLKTYGFHGIHGRALPIATGVRLARPELPV
                     IAIMGDGDCFSIGGNHWLHAIRYNINALVLVLDNEVYALTKMQASPTTPDGWKTNTTP
                     HGTYLHPLNPLSAVMGMTNVSFVAQTATWLPAHLDETIEKAWDHHGLSFVRILQRCPV
                     FMPKAFGEGGLNFPVLFLENEDGIPVHPLLLKRGRAQAHNHRDINEAQRIAVAVDPAP
                     MGLIYQNPDVPAYEEIRWQRKDRLDRATFLQRLDSELDRYTVS"
     gene            13436..14902
                     /locus_tag="VFG22_05460"
     CDS             13436..14902
                     /locus_tag="VFG22_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415721.1"
                     /translation="MSAMKEQILVTIEEVWRQHRAALEDALTEAIDALQNLLRLDEYH
                     RHGHDPEQLERALGPLANTSLDLGSLSRMLDQGARSRTMAPDRVERIEQLILTLGEMK
                     ELWSNAGLDTASIEMGENEDEVRKRAEAYLNRLALVFRALRITQLEVRSKYDSQAHDA
                     IFAQFDWREIGPGEIQSAPPFVVLARMDGDAGPQLHRLVSLLDTGMPLKLVALRSSVR
                     DAPAQLKIETLPLAMHDIHFVQSCTVVPEFEKQVLACLAAPRPGVISLLCPRGDEEQA
                     AFHARAERAVRSRAFPICVYDPDRDPSFVMCFDLSSNPSPELPWTTETIPGQDPNGQA
                     VELEQPFLFAQFAASEPEFASDLTDAPANSDDLVSLSEYLGFSRRQRIGKLPFISLAD
                     EPGPIVRKVVSQALVRQSAERVQLWRMLQEISGIDNPYVKQGQTRVQNELRAQHEAEL
                     ESLRRELENDVALREKAAVVSAVHKLVARLTGIDPSRN"
     gene            14963..15829
                     /locus_tag="VFG22_05465"
     CDS             14963..15829
                     /locus_tag="VFG22_05465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HET6415722.1"
                     /translation="MLFLTAVAAISGLTVILASALVLANKKLYVYEDPRIDVVEEMLP
                     HANCGACGFPGCRPFAEALTTGQALPGKCTVSSDGDRAEIANFLGVALGAEEKVVARL
                     ACAGGSNVARNHARYHGEQTCRAAARVAGGGKGCFWGCLGLGDCDRACDFEAIQMNAH
                     NLPVVDESKCTACGDCVDVCPKDLFSLHKVSHRLWVACSSQEAGDEVLEDCEVACTTC
                     GRCEMDAPRGLITMRKNLPVVDYSRPHQTRSPIERCPTGAIVWIDAKSGPVKGPAAKK
                     IIRKGTLSDAPT"
     gene            15862..20847
                     /locus_tag="VFG22_05470"
     CDS             15862..20847
                     /locus_tag="VFG22_05470"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HET6415723.1"
                     /translation="MSPRFFGKKTQEHGRDGERQFKYPGSRAALDGNSAVIMCEREST
                     DAAGAYPITPSTQMGEFWAEAAAAGHINVSGRPLIFIEPEGEHAAAAVTAGMSMTGMR
                     AANFSSGQGIAYMHESLYAATGKRLTYVLNIGARAMTKATLNVHAGHDDYHCVDDTGF
                     FQMFAKNAQSAADLNIISHRIAELALTPGIIAQDGFLTTHLIESLMVPERELIAEYLG
                     RPDDIIDTPTPAQKIIYGETRRRIPMLWDVDNPVMSGLVQNQDSYMQSVAAQRPFFFD
                     HIQALTARAFEEYYELTGRRYERVMTYRADDADYLIVGQGSMVPSAEAVVDYLRETRG
                     LKVGVVDMLMFRPFPSDLIGQIIRGKKGVAVLERLDQPLAVDLPLMREIRATVSKCME
                     NAKDPKNPAFPELPTYASLQDAPALYSGSFGMGSRDLQPEGIVGAVENMLEKGARKKL
                     FYLSIDFVRDEAITPKQEIYQQTIEAAYPNVKGLVVRGSENPNLMPKDSITVRFHSIG
                     GWGAITTGKNLAMTLFDLLGYHMKANPKYGSEKKGQPTTYYLSAAPEPIKINCEYFYV
                     DVVLSPDPNVFGHTNALAGLKEGGVFIIQSDKASSEEMWESIPPIYQKIIIDKKIKVY
                     YLDAFKIARDEATDSDLQLRMQGIAFQGAFFAASPVIRQAGLSEEKLLDAIHDQLQEK
                     FGSKGRRVVEDNIRVVKRGFDEIRELVPGPITRLKAESGRAGPRLPMMVKSLPQSKAP
                     MTDIHRFWEQTGSFYARGMGNDNLADPFIGLGVMPPSSALFRDMTGIRFNHPEWVPEN
                     CTACGNCYTACPDTALPGLVNEVGQVLDTIVARVRKNGRGAEIEHLPKAAKVMERNLH
                     ALFKKAEETESVALLIDQAMAETLKQSELQGGEKEKLRKELDYFKEELNGFQFALTRP
                     HYTLAEKDQAGSGGLLSITVNPYTCKGCMECVEVCNDDALRSVKQTDESVQKLRDNWD
                     LWTDLPTTPKKYIRVDDLEEGIGTLESILLEKSNYLPFTSGDGACLGCSEKTALHLFV
                     ATVDALMQPRIAKHLSHLTELIGRLKKHIQLRLAEEINVSDANVMAKVMDDMGDRDVT
                     LAVIAQRIESMGGGQPIDQDWLRRMTELLTRLETLKWKYTDGTTGRGRTNMGMVNSTG
                     CTSVWGSTYPFNPYPFPWANHLFQDSPSMAMGIFEGHMAKMADGFKAVRTAELELDNK
                     YKPAEHDDFFTYFDWRQFSDQEWELCPPVVAVGGDGAMYDIGFQNLSRLMASGKPIKV
                     VVVDTQVYSNTGGQACTSGFIGQVSDMAQFGKAIQGKQEPRKEIGLIGLAHRNTYVMQ
                     STIAHSSHMIEGFIRGLKARRPALFNIYSTCQPEHGIGDEMSTAQSKLAVESRAYPLF
                     RYDPDAGKTLQECLDLEGNPDADQDWPLYRIKYQEDGVEKEMELPLTFADFAITETRF
                     RKQFRVAPPDTWNENMVPLAEFLEMDEDEREGIFPFVWMVDKEEHLTRLLVAQPIVES
                     CEDRLDFWNMLRSLVGADQPSTESLVEQVRQDVVGKLVSELMQIAAGSGEGFSAPATG
                     EVAAAATPAVAPAAASAAAPAAGATGDYLAPWIDTANCTACDECIQLNSKVFVYNADK
                     KAIIKNAEAGSYKDLVKAAERCTARVIHPGLPRNRSEKGIEKWIKRGEKYN"
     gene            20870..22219
                     /gene="rsxC"
                     /locus_tag="VFG22_05475"
     CDS             20870..22219
                     /gene="rsxC"
                     /locus_tag="VFG22_05475"
                     /inference="COORDINATES: protein motif:HMM:NF003454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HET6415724.1"
                     /translation="MRLLGTKTFRHGVHPPESKDETSGLPIRQFPFAPVLIIPLVQHM
                     GAPSIPVVAEGDEVSRGQRIARADGFMSVSMHASASGIVRRIALAPSIGGRMVPAVYL
                     EPFPGSTQEIEDGTPCPLETSTPDEIIAAIQDAGVVGLGGAAFPTHVKLQVPEGKFVD
                     TLIINGVECEPYLTTDHRVMLEQSDDVFMGIRYLLKVSGAKKVIVAVEANKPDAAEHL
                     RKNFPSDLDGSVEVLQVKYPQGAEKMLFAALLGREVPSGGLPLDIHALCCNVATTAEI
                     GRLLPLGRGIQERVITVTGPAVKRKGNYRIPIGTPLRFLLEQVGVEEDVTRVFLGGPM
                     MGAAASSLDISITKGTSGVTAFTDSAAERTHSPVYPCIRCGYCVDACPIFLDPSLLGR
                     LAANEEFSAMAERHNLMDCFECGSCSFVCPSHIPLVQRFRAAKTAVRKANAPKAGAA"
     gene            22219..>22896
                     /locus_tag="VFG22_05480"
     CDS             22219..>22896
                     /locus_tag="VFG22_05480"
                     /inference="COORDINATES: protein motif:HMM:NF015100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HET6415725.1"
                     /translation="MTTPGKTLEIRTSPHILSGNSVDTIMFNVVLALLPVTAFAVYAF
                     GLAGLLTLSMALTSCVLTEHLICRATGKPSTVGDWSVTITGLLYGLTLPPGLPLWMVA
                     VGGLICVAMGKALFGGLGYNTFNPALVGRAVLQAAFPVAMTTWIPGFMAGRFSSLPSS
                     TLTLPLTEPAVDAITSATPLALWKFSHEATPISELSLGFVSGSAGETSSVLILLGGLY
                     LIARNMIN"
BASE COUNT         4520 a         7217 c         7032 g         4127 t
ORIGIN      
        1 gtgccacata cggggaaccc ggctccgttc cggccgtggc gtcgagcatg gcagcggaat
       61 cgcggacggt ccggctgatc acgtgcgagg tggactgtcc ttcatactcc cagctgaagt
      121 cggggccgat cgggttccgc cctcggctgg gcttcagacc gaacacacca caggccgcgg
      181 cgtgcattcg gatggagccc ccttcatcgg tggcagaggc catgggaacg atcccggcgg
      241 ccaccgccgc ggccgagccg ccgctcgatc cgcccgcaga gcgggttacg tcccaggggt
      301 ttcgtgtggg tccccaggcc ttgggcatgg tgaagaccga gagcttgtat tccggggtgt
      361 tggtcttgcc gaacaccacg acgccggcgc ggaggaaacg acggacgatt tcggcggtgt
      421 gctcggagat ctcccccttg tggagccggc ttccattgct catcggcgtc ccttggaggg
      481 cgtggtgcgc gtctttgagc aagaacggca cgcctcgaaa tggcccgctg agatcctcgc
      541 gaagcctcct gtgcgcgtgc tcgtagaggg gagtgacgat ggcgttgagc gccgggttca
      601 gcttctccgc ccgaaggatc gccgcctcca agacctcctt cggttggacc tctccgcgag
      661 cgatgagctc ggcgagccct agcccatcgt agtgaacgta gtcgtcaaac acttcggctc
      721 acccttcccc tgcatcgcta tgtgggcgcg ccctccgggc gcgggaattg agaatcgcga
      781 tgatggaacc gacgctcaca aaaccgatca cataggcgcc gccatggctg aggatgtcgg
      841 cgtgcacctg gtagagcgcg aggctcgtac cgatccccaa gccgtaagca aaccagggtt
      901 gctcacgcca ctggatcttc tggcgcttgg tgacgagcgc aaccagcggc caaaatgaag
      961 tcgatgcagc gtaggagcga tacggctctc cgagctcttg aaagagtctt cgatcttggt
     1021 gcagcccccc gaccaacgaa agcaccacga cgcctatgaa gaaaacgaag ggcgccggtg
     1081 acggtgcaag gagcgcgtgg gcggccgccc aaatcgcgat gccggaaaag aacgggtgac
     1141 gagtgacttg ttgaaccccg cgtgctggca ctactcggtg acggaacgtc gccatcggta
     1201 ggcgcgggta gaccagaacg gcagtgatga acagcgagaa accggcgacc gagagcgcga
     1261 tcagtgcgcc gcgcaccggc ggaatcgtga cgagcagcgg agcttgcggc tcggcgacac
     1321 ggtgctctgc aacgtagtga accagcgccg cgaaagtgat gatcgcgatg acggaataga
     1381 cgaagacgaa accgccctca ccgaggcgcc gcacgagccg ctctcgcact ggccgcatcg
     1441 tgagcccgat atgggttcca ccgaagagaa gccacaagag agccgtgatc catccttctt
     1501 ccatcagagc tgcgcatcgt acgacggttt gtgccggttc tccaggggtg ctgctagact
     1561 tccgcgcgtc atgaaggtgc tgcacgcagg agccgcgccc tagaggtcga ggcagcgtgc
     1621 gtttcgtcgc tcacaagtcg cacatctacc aacggctcgc tcaatcgggt tggtcgcacg
     1681 ccaatgtgtc ggcggtatat tgcggcgcta cactggcgat tgcgctgctg gtcgcagcgc
     1741 taagctgagc acttgagcgt accccggggc cacgcaagcg ccctcgagca ggcctcgctc
     1801 ccgcagcatg gtgtgaaact ttgggagatt gtagtgggga acggtcatca gcaggtgatg
     1861 ctccagatga aactgaactc ggtttggagc gatgaacagc cgttcgaaag gacccgcaag
     1921 cacggttctc gtctggccga tcggatcgct cgggtccggg acgaccgcgt gctcggcgat
     1981 ggagcggatg cgggcgacca gcttgttggt cgtgagccaa gcccctagcc agagcagata
     2041 gagcagcggc tgcccgatcg accagaggaa agcaaagagg atgagattgg tgacgagtgt
     2101 accgacgacc atccgaatcg caggcccgcg ctccaggcgc aacggtaagg ttcccgcgtc
     2161 gacttgctcg ccgcgcagga ctttcacgat cagatagctg gtcccgatga gctgcttgag
     2221 gcccgtgacg ccgaacaggt cacgcatcac ctttcgcgcc atgcttcgcc tggacaccgg
     2281 aaacgcggtc gcgagcgcta gatccggatc gccctccgtc caggtctttg catgatgctc
     2341 cagatgatgg gttcggtaca tgtcggcgct gagatagatg ggataaccgc agagccagtt
     2401 tcccacgaag tcgtttagct tccgattctt gaagagcgtc cgatgggcgc tctcatgcat
     2461 cacgacggcg aacccgagct gccgcgtgcc gatcacgaaa agggccagcg cgacagtgag
     2521 tacgtttggc accaatgcaa ccatcgtcat cgacgccgcg atcaggccat agttgacgaa
     2581 gacggtgagc caggcgcgat gcgcgcgaat gaccctcagc gcgcggatct cttcactcgt
     2641 aaacgcctct agccaagcct tgctgccctt gcccactcgc tagcctcgga ggatgtactg
     2701 ctcgagctgc tcgatggtca tgtcttgctc ggcgacgatg ctgcgcacga gatcgccgat
     2761 cgacaagatg ccgagtagct ctccgtcctc catgacgacc aagtgtcgcg ttcgcttgcc
     2821 ggtcatgatc gccatgcaat cgcgagtggt cttgtctcgt gtcaccgaca agacctcgac
     2881 gtccatcgcg tcgcgcacgg gggtggtggg agaggccttg cctctgagga agacgtttcg
     2941 tgcgtagtct cgctcggtga ggagtccgat caccttgccg ccctcccgta cgaccagtgc
     3001 cccgatgtcg aggtcggaca tgcgcttgat ggcttcgaag accgtggcgt ccggcgcgat
     3061 cgcttcgacg ttgtggccct tccgctctag caattgagct accgtgatca tctcgtgcct
     3121 cgctttgggc gaggatagca ccttccgctg atgcgtagaa gcatgccctc gctgcgggtt
     3181 tgacgcctgg gactggcccg gatgcaggaa aggctcagcg gttcacgacc gcaatgctcc
     3241 aggcgatgat caactgagcg gcatagtaaa gaggcaagcc ccagagtcgg ttcacgaatc
     3301 cacgtttgac gaagcggtcg cgcaccactg caatgtcgga ggcggtgaac atgagcgcgc
     3361 cgactgggac catccagggg gcgccggcac cccccgctcc gaccgcaacg acgcacatcc
     3421 cggcgatgac cagcatgtag atcccgattg ggacgcgcat ctcgacggcg tgtgggaata
     3481 cccatcgcgc aacgaagaac atgagcaggc ccatcgccaa cccagcgacc aacgcatagg
     3541 aagagttaaa cggcagtaag ccgaacgcaa atacgtacgc cacgtggccg agcaagaacg
     3601 acgtcaggcc tgcgacgaag tactgcctcg cccttccgag caaacacacg tcgccgatgg
     3661 cgccgaggac gagaccggcc agcaccgtct tcccgtagcg agactcgagc gctccgaagc
     3721 cgacggcggc tacgaggaac ccgaacgatg cgaatggctt gcctaagaag cgcaatgcgc
     3781 gccgatcctt ccgctctccg actagcagaa cggcaaggcc cagggcggtc atcagctcac
     3841 ccactccgtt gagtgtcacg atactgtcct ctcagcctcc gaaagaccca accaggactc
     3901 ctcctcttcc agaaactgca gcggatcgag ctctggcttc accttctcgt agatggtcgg
     3961 aaaggtccca tccgcgttgt acttggctcg catctctcgc cagagtccgt ggtcgaacat
     4021 ctcctcgaac tctgcttcag tgctgaacac gtcgcagtac gtatgaagga agcctccgag
     4081 tgaacgggcc ttctgctcga gtcgccggac cttcgttacc gcaggaaagc gttcttcgcc
     4141 ctctttgatg cttcgcggaa agccatagat tccgatatcg agaaacgtct ccgtctcggc
     4201 atggccggaa tagggctttc cgcgattcgc cgggcgccgg acgagacctc cgtggtcgat
     4261 cacgcggcac ggataggcga gcagggggta gatctcgaat tcccggtgca cgtactcgac
     4321 catttcttcg aaatactcgg ctgggaacgc gtactcctgc cagacctgct ttcggatcgt
     4381 gttttcacgt tcggcctgcg gccgcgtgcc tttgagaaag gtggtgttgg gcggcagcat
     4441 ccatcccatg gtgtagcgaa accagggctc gttggcggtc ggaagtatct gggccaggca
     4501 catgcacatg ctcctgtcat ggcgcatcag ataatcgtac ataggaacga gctcttcaca
     4561 gcgctcgccc tcttcaagct ccagcatgct ctccacgtgc ttgtagaaaa gcggcttgta
     4621 gtaccgactg agcggattga tcggcaggct ccccgcgcgc acctcctcgg gcgatgccaa
     4681 atgcccctcc atgacgaccg cgcggtcctt gccgaagatg atggtctcga tgaaatgcgg
     4741 cgggtcggcg tcagacgcga gtcgacgata ctcggcggca tacgcctcga gcgaatgaaa
     4801 cggccgatac actaggcgca cgtgtgaggg cgcggggatg acgcgcatct cgattgcgac
     4861 gagaaggccg agagtgccgt ggctccacgg aagcgccctg aaaagctcgg catgttcgtt
     4921 gtcggccgtc gcccgtacca agtcgccctc tgcggttacg atctcatagg cggtcacggt
     4981 gtcgtggacc agaccgcaaa cgtgtgagtg cgtcgtcatg ccgagcgcca tcaccagccc
     5041 gccgagcgtc gcatccttca tctcgatcgt cgcttcgagt tggagcccct tctcgtcgag
     5101 gtgctgcgcc acgtcgccga ccgtcgcaaa cggctcgagc cgtacgatgc cggcattctc
     5161 gtcaacttcg agaaccgacg tcagatcgtt catctgtatg gtctccgatc ccgacttgtc
     5221 ggcgacgcgt gtgttcaggg aagcggcacc tttgcggtcg gtccgaagcg gtcgttgcgc
     5281 ccaagcgtca ccttgccggc gtgccttcgc atagcgccgc acctcggcct gcacgcgctc
     5341 gacgcgcgca tcgtgacctt cgggccgcac aggcttcgca aacgcatttc gcaccctgtc
     5401 ccatccactc gcgagcgtgc tgagcggcaa agcgactccg atgatcatca gttcgcggtg
     5461 ctcgtcggcc caacgcttcg cgacgtcagc ggcgtccttg aagttcatgc gcctggtttt
     5521 gaaacggttc gcaggcaaat gaaaggtgct acatagattt tgtcagccct tcgacgaagg
     5581 gcagcgagga acgtcaacgg agaaagcacg gtcatgagcg atgagaagcc gctgcccctg
     5641 gcaggtgtca gggtcatcga attcacccac atggtgatgg ggccggccgc cggtttgatg
     5701 ctggccgaca tgggcgcgga cgtgatcaag ctcgagccgg tacacggcga caagacgcgc
     5761 tcgttgcccg gttctggcgc gggctacttt ccggcctaca accggaacaa gcgctccctc
     5821 tgcatcgatt tgaagtccga cgaaggcaag gccgccgtgt tgcggctcgt cgacactgca
     5881 gacgtgatcg tcgagaactt tcgccccggc acgatggacc ggctcgggtt cggctacgaa
     5941 gagctccgaa agcgcaacaa gggcctcatc tactgctctg aaaaggggtt cctcgcgggt
     6001 ccttacgaag agagaacagc catggatgag gtcgcgcaaa tgatgggcgg cctggcatac
     6061 atgacggggc ctcccggtcg gccgcttcgg gcgggcgcgt cggtcatcga cgtccagggc
     6121 ggcatgtttg gtgcgatggg cgttctcgcg gcgcttctgc aacgcaatct cacgggcgaa
     6181 gggcagaaag tgatctcatc gctattcgaa agcaccgtat tcctagtcgg ccagcacatg
     6241 gctcagtacg cggtcacggg cgaggccgcg gcgcccatgc ccgctcgaat ctccgcctgg
     6301 gcgatctacc aaatcttcgc aaccaaggac gacgacctgg tgttcgtcgg catcgtgagt
     6361 gacaagcaat gggaattgct ttgtggtgcg ttcggattgg acgagatggc ggcggacgca
     6421 actctcaaga ccaacgccgg ccgtgtagca gaaaaggatc gcttgcttcc catgatcagc
     6481 gacgtcttcg ccagctactc gaaggccgag ctcatggcga aactggaatc gcgcggattg
     6541 ccattcgcgg cgatcacgcg tcccgaggag ctgttcgacg atccacatct gaacgctgga
     6601 gccgggctct tgccgttcac cgtcaccgat ggcgatcgaa agggaaacga agccaagctt
     6661 cctgccctac ccctggaaat ggatggaaag cgttttggag tacgccgtcc ggtgccgcga
     6721 gccggggagc acagtcgaga ggttctgatc gaggccggct accccgagga ggcgatcgat
     6781 tcgatgctgg ccgccgggat cgtgacggcc gagtagccag gccaccctcc cgaggatccc
     6841 ctccaggccc ttgggtgtgt gcttttgtga gagtgtgcta cgccccgccc ttcatgtctg
     6901 agcaacttag cctagagcag gcccccgcgg gcctcggtgc agccctgcgt aacgccggat
     6961 tcgagagctt taccgcgatc cagcacgcga tcttgaagcc ggagctggag gaccgggacc
     7021 tgcggatctc ctcccaaacc ggctccggca agaccgttgc cctcggcctt ctcctcgcgc
     7081 caagcgtagc gaatgcggct accaccgcgc gtccccaaaa atcccgcaag tcggcccccg
     7141 cctcgccagt cgctctcctg atcgctccga cccgcgagct tgcagggcaa ctcgggagag
     7201 agctcagctg gctctacaag ccgctcgccg ctcgggtgac cgtcgtcacc gggggaacca
     7261 gcgtcatgca agagcgctcc gagctctact ctctccctca ggtcgtgatc ggaacgcccg
     7321 gccgcctcct cgatcatctg cgtgctggcg ccttgaacct cgatcagctc gcgacgctcg
     7381 cgctcgacga agcggacgag atgctcgaca tgaacttcga agaagagctg gatggcattc
     7441 tcgggttcgc gcccgacgca cgaaggaccc acctcgtctc ggctacgttt ccgcgcgaag
     7501 tcgtgaggct ggcggaccgc cttcaaaagg accctgtctc ggttcaggga acgacgctcg
     7561 gaaaggcaaa tgccgacatc gagcacgtcg tgatgcatgt gcggcaggcc gaccggttca
     7621 atgcggcgat caacgtgctc ctccgctacc cggaagacaa gacactgctc ttcgtgcgca
     7681 cgcgaacgga aacggtacgg ctcgccgatg agctatgcgc agcgggactt gccgcgcgag
     7741 ccctgaatgg agagatgaca cagcgcgagc gaactgccac gttcacacag tttcggaacg
     7801 gcaccattcg ctacctggtc gcgaccgatg tcgcggcgcg cggactcgac gtccaagaca
     7861 ttggacgggt cattcaggtc gaccttcccg acaatgctga cgtgttcacc catcgaagcg
     7921 gacgcactgg tcgagctggg cgaaagggca cgaatgtgct cttcgcggct tcgcgcgcga
     7981 cggctcgcgt gaggaatact ctgcgagcag ccggagtcga agcgcggatc ctgccaattc
     8041 cgacccccga agagatcttc gccgccgcgg atgagcgaat cggtgaaagc ttcttgcagg
     8101 acctcgagag ccaatccgac aagaccaacc tacgggccag agcccttgcc gaacgtctac
     8161 tcgccaagca ctcgccggcc gagctggtcg cgtgcctcct ccaacgcgag gcagaacgca
     8221 gcatgggaac gccgaagcag gtccgagatg attcgcagcc ggcgcatcgc gaagcaaggc
     8281 cgcgcaagac gggcccgagg tcgcggtcgc gtcagcgccc gcaaggcgac ttcattacct
     8341 atcaggtcag ctggggcgag caggcaggcg cggacacacg tcggcttcta gccatggtct
     8401 gccggcgagg tggcgtatcg agtgaggtgg tgggttcgat tcgcgtggct tcgaacagct
     8461 cgacggtgga catcgtcgcc agcgaggccc agcgcttcga gcgcgccgtc gcgcgtcctg
     8521 acaagcgaaa cccgcgcgcc aaatttcgcc gctgggactc gagcgcgagt gccaagcgcc
     8581 cccgcaaccg ctctgcagac gcccgctgcg cctgagctgc atgctcgcca gcgacgagcc
     8641 tcgatagtcc gggtgtcgtt ctcgtcctcg gggagctaag cttcctcgat gaggcgagtc
     8701 ctatttttcg ctaccctgct ctttctggtc ctcggttgca aagaccgcga ggagacgagc
     8761 gcggtcccga cggccaacga ggctcttgcc gcgacagcgg aagagggatc cggcgtgccc
     8821 gaggtcggca acgacttcga tctcggcgac gaggcagagg ctaggccaga tggcgaggat
     8881 gccgagaagg caagcgtcga gtcagccgac aacgctgcgc cacccgtacg cgatctcggg
     8941 gcggagcttc gagccgcgct ggggagcccg gtcgactgtc tcaatgacta tcagccgagc
     9001 ccgggcaccg tcgtccgagt cagcgtcagc gcaatcgtcc ggcaatcggg agtcgtgatc
     9061 gagccgagcg caaacggcgc aggcctctcg gcaaacgatc ggcgatgcat cgagcaacgc
     9121 gccggagatg tcgtcttggc cccattcgag gccgagacct cacgacccgt ttcgacctac
     9181 gtagacctcg tcgttggggg aggccccgtg gaggagaccg acgtcggatt gccggatcca
     9241 aagctgaagg acgtccgggg catcgagggc ggaatcgtcg ataccatcac gccgagcggc
     9301 attcccgtcg agaaactgcc ggcggatgcg ccggtaggtc ctcgcggtga ccctgtcgaa
     9361 ggaccgaagg gtgtcccgat cacaggtccc agcccgaaac cgatcgaggg gtaccaactc
     9421 gacgaaaaag aaggcgccga gcgctggacg gaatgaagcc cgcgccgcgc ccgccgatgc
     9481 tccgctactt ggcggcgccg ccctgcgcgg gcgggtactt tgcgaggacg gcatctacgg
     9541 ccttcatgat gatctcgtcg ttcccggcct cggagaagat gtcttccgtg ccgtagccgt
     9601 gccaaacgag tcgcttcttt acgcggtcgc ccatctgaat gacgaggcta ccctcgacgt
     9661 agttctcggt ctgaaactct ccgggaccgt accagcccca tcccgtgaag tactcggcct
     9721 cttgtcgctg ctggccaccg agtgtgaagc tcacctgcaa gtccgcctcg tccatgcccg
     9781 ccggctcgag gcccttctgc tgaaggacca tcgaaatgcg atcttggata ttgcggccag
     9841 tgatttcgct gtagcccggc atgttgtccg cgtagctcgg cgggggtgtc tgagcgaacg
     9901 tcttgtagct cgcgaaatcg accgtgtcgt cgtagctgat gttggtcttg acctgtcccg
     9961 cgcatccgac gaaaacaaac gtggcaatta gcagtaccgt ttttccagtg ttgtgcatct
    10021 aagtggctcc tcgccccagg cctactgcgc atcgatgacc ccgtccaggc gccaagggag
    10081 cggagtctcg cggtcgaatc ccgcccgacg ggacgagtcg ttggcgacta gcactcgtcg
    10141 tccaccgcga ggtcttcgtc tcggcaggga tagtcttcag gggggacctt ttgcgtccat
    10201 gcgtcgagct ctgcgcagga caggcccgcg acgcaggccg cccgatcgtc cacagcggtg
    10261 ccacaggcaa tggaggcatc gctcgcttcg ttgaaatccc cctcgcattc ctccatgcac
    10321 gactgataaa tcctatcggt gcatgaggaa atgaccgaac aggaaggacc gcaattgctg
    10381 tcgttgcgcc cgcaacccgc ggaggcgatc gcaacgatgg cgacgacggg gatgattgca
    10441 ctggcttgca cccaagagcc tcctgcggac actctatgcc aggtctccgc ctaaactcca
    10501 gggcgtccga ttgctcggcc cggatccttc atgttagaga ctcggggtcc aaaggaagat
    10561 cctatgactg ctccccgtcg cgttcgactc ccagagcata ccgtcgagat cgtcagcgac
    10621 gctggagaag gcgcacagaa agctgcaatc gcctttgctc aagtctgcgc gaaggcaggg
    10681 aacggcctct ggaccgtcga gatcattccg tccgagatcc gccccccgcc gcatacgacg
    10741 ggaagcgcca gtggcaaccg aatccgcctg gcggagcgcg accgggtcac gaatgcaggc
    10801 aacgaagcca acgtggtgct ggcgttcaac gagatggccc tgctctcccg gctcgaggct
    10861 ggaagcctcg ctgacgacgt tctggtgctg atcgacgatc agtgggcaac gcacgaggtc
    10921 gagaagtatc agacctcgta ccgcgaggtg ctggacacct tccgcgcgcg cggcgcgacg
    10981 atcatcgaag ttccgatgga gcgagaaacc cttcgccacg ccgaggatgc acagcagggc
    11041 aagaacatgt tcgcggtggg cctcttgtgt gccctctata gcaaggacct cgatgttctc
    11101 gacgacgtga ttcgcgtcat cttcaagagc aaagcgcctg cggtgatcga gctggcggtg
    11161 aggctcgcgg ccgcgggcta tcagtacgga gtcacgaact tcaaggaatg cttcgaaatc
    11221 gaagcgcatc cgactgatgt ccccaaggtg gcgatgaatg ggaataccgc gcttgcgctc
    11281 ggcgcaatct ctgccgggtt cgagctctgc tcgatgtacc cgatcacgcc ggcgacttcc
    11341 gcgtcccacg ccttgtctga gacattcgaa gacttcgggg gcttcgtgca tcaggccgaa
    11401 gacgagatcg cggcgattgg ggtggccctc ggcgcttcgt atgcgggaaa gaccgcgctc
    11461 acgatcacgt ctggaccagg gatggccttg aagacggagt tccagggctt ggccgtcatg
    11521 accgagacac cgctggtcat cgtcgatgtt cagcgcggag ggccgagcac cggtttgccg
    11581 actaaaatcg agcagtcgga tctactgcac gcactctacg gcgctccggg tgacgcgccg
    11641 aaggtggtga tcgcgccgag cactatcgaa gagtgttatt acattttggc ggtcgcgctg
    11701 aagatcgcgg aggagttccg gatgctggtg atcgtcctgt ccgacgcgaa cctagcgaca
    11761 ggtcagcagc tcttcgatcg acccgatgtt tccaagacgc cgcggcgaga gccgctggac
    11821 ctcagcccgg ttccgcctgg cgcgctgccc tacgactggg atccaacgac cggtctttcg
    11881 gagcgtcgca tccccgggca acccggcggg atgggagtga cgacctcgct caaccacgat
    11941 cgcaacggca aggcttgcta cgacgccgag cggaaccagc gtggccacga catgcgcagc
    12001 cggaaactcg ctgcgctgca gcaaagcctg cagccgccga gtgtgtacgg agcccccgag
    12061 ggggaccttc ttctcgtggg ctgggggagc acgagaggcg cgatcgagga agccgtggac
    12121 gcggcacgcg gtgaaggcct cgcggtttcc tcgatacaca ttcagtttct gagccccctt
    12181 cccccggggc tcaaagacat cttcgcgaat tttcgtaagg tcatcacggt cgagctcaac
    12241 tacagcgacg atttgaacga ccccttgatc acgagcgaaa atcggcgcta cggacagctg
    12301 gcatggatcc ttcgagcggc gaccctccac gacatcgact gctttgccaa ggtgcccggc
    12361 cgcccctaca tgcccgtaga gattctcggt gaggtcaaac gcatcctcgg cgagcaaacg
    12421 gaggtcgaag atccgtccga gagattcgtc gctagcgagg gagcaaaccg atgatatcaa
    12481 tgcttgccga agagagtctc ctcgagatct gcgcgatcga aacgcactcc ctcgacgagt
    12541 acgaatccga gcaaccccgc tggtgcaaag gatgcggcga ccatggggtc cttgcctcgc
    12601 ttcaacgtgt gctccgcaag aacaacctcg agccggagaa cgtggtttcg gtttcgggga
    12661 tcggatgctc gagccgcctc cctcactatc tcaagaccta cgggttccat ggcatccacg
    12721 gtcgggcctt gccgatcgct acgggggtgc gccttgcgcg tccggagctg cccgtcattg
    12781 cgatcatggg cgacggcgac tgtttcagca tcggcggcaa ccattggttg catgccatcc
    12841 gctacaacat caacgcgctc gtcctcgtgc tggacaacga ggtctatgcg ctcaccaaga
    12901 tgcaggcctc accgacgacg cccgacggat ggaagaccaa cacgacgccg catggcactt
    12961 accttcaccc cttgaaccct ctgagcgcgg tcatgggaat gaccaacgtc tcattcgtcg
    13021 cccagacggc cacctggctt cccgcccacc tcgacgagac catcgagaag gcctgggacc
    13081 atcatgggct ctccttcgtt cgtattcttc agcgttgccc ggtgttcatg cccaaggcct
    13141 ttggtgaggg cggactgaac tttccagtcc tgttcctcga gaacgaggac ggcatcccgg
    13201 ttcatccctt gctgctcaag cgcggccgcg ctcaggcaca caaccatcga gacatcaacg
    13261 aggctcagcg catcgcagtg gccgtggacc ccgccccgat gggtctcatc taccaaaacc
    13321 ccgacgtccc tgcctacgag gagattcgat ggcaacgtaa ggatcggcta gaccgagcca
    13381 cgttcctcca gcgtctcgac tctgagctcg atcgatatac ggtgagctaa ttcatgtgtc
    13441 cgcgatgaaa gagcagatcc tagtcaccat cgaagaggtc tggcgccagc atcgcgccgc
    13501 gctcgaggat gcgctcaccg aagccatcga cgcgctacag aaccttcttc ggctcgacga
    13561 gtatcaccgg catgggcacg atcccgagca actcgagcgg gctctcgggc cgttggcgaa
    13621 caccagcctc gacctcggct ccctgtcccg catgcttgac caaggcgctc gctctaggac
    13681 gatggcgccg gatcgagtcg agcgaatcga acagctcatc ctgactctcg gagagatgaa
    13741 ggagctctgg tcgaacgccg ggctcgatac ggcttccatc gagatgggcg aaaacgagga
    13801 tgaagtccgc aagcgcgccg aggcttactt gaatcggctc gcattggttt ttcgcgcgct
    13861 gcgcatcacc cagctcgagg tccgctcgaa gtacgattca caagcccacg acgccatctt
    13921 cgcacaattc gactggcgag agatcggtcc cggcgagatc cagtcagctc cgccttttgt
    13981 cgtcttggcg cgcatggacg gcgacgcggg tccgcaactg catagactgg tgtcgttgct
    14041 cgatacgggc atgccactca agctcgtcgc ccttcgctcg agcgtccgcg atgcacccgc
    14101 ccagttgaag atcgaaacgc tcccgcttgc gatgcacgac attcatttcg ttcagagctg
    14161 cacggtcgtc ccagagttcg agaagcaagt gctcgcctgc ctcgccgcac cgcgcccggg
    14221 ggtgatttcg ctgctctgcc cgcgcggcga cgaggagcag gccgcgtttc acgcccgcgc
    14281 cgagcgcgcc gttcgttctc gcgccttccc catttgcgtc tacgatccag atcgagaccc
    14341 gagcttcgtg atgtgcttcg acctctcttc gaacccttcg ccggagctcc cgtggactac
    14401 ggagacgatt cccggccagg atcccaatgg gcaagcggtc gagctcgagc agccgttcct
    14461 cttcgcccag ttcgccgctt ccgaaccgga attcgcatcc gatctcacag acgcgcccgc
    14521 aaactccgac gatctggttt cgctgagcga gtatctcggg ttctcgcgca ggcagcggat
    14581 cgggaagctt ccgttcatat cgttggcgga cgaacctggt ccaatcgttc gcaaagtcgt
    14641 ttcccaggcg ctcgttcgtc agtccgccga gcgggttcaa ctctggcgca tgcttcaaga
    14701 gatctctggg atcgacaatc cgtatgtgaa gcagggtcaa acgagggttc aaaacgaact
    14761 gcgcgcgcag cacgaggccg agctcgaaag cctgcgccgg gaattggaaa acgatgttgc
    14821 cctacgcgaa aaggcagcgg tggtctccgc cgtgcacaag ctcgtcgccc gcctcaccgg
    14881 catcgaccct tcgagaaact gaggctacac tcccgaacgg agcggcgagc ctcagctgga
    14941 ggctacgcat agtttcacaa tgatgctgtt tctcaccgca gtggcggcca tcagcggcct
    15001 caccgtcatc ctggcctcgg ccttggtctt ggcgaacaag aagctctatg tttacgagga
    15061 cccgcggatc gacgtggtcg aggaaatgct acctcatgcg aactgcggtg cctgcggctt
    15121 ccctggctgc cgaccgttcg ctgaagccct gaccaccggg caggcgctgc cgggcaagtg
    15181 caccgtgagc agcgatggag acagagcgga gatcgcgaat ttcctgggcg ttgcactggg
    15241 cgccgaggag aaggtcgtgg cgcgactcgc ctgcgcaggc ggcagcaacg tcgcccgtaa
    15301 ccatgcccga taccacggag agcagacctg ccgcgcggct gcacgagtgg cgggcggcgg
    15361 caagggctgc ttctggggct gcctggggct gggagactgc gatcgagcct gcgacttcga
    15421 ggccatccag atgaacgcgc acaatctgcc ggtggtcgat gagtccaagt gcaccgcctg
    15481 tggggattgc gtcgacgtct gccccaagga tttgttctct cttcacaaag tgagccatcg
    15541 tctctgggtc gcctgtagct cccaagaggc aggtgacgaa gttctcgagg actgtgaggt
    15601 tgcgtgcaca acttgcggcc gatgcgaaat ggatgcgcct cggggcctca tcacgatgcg
    15661 aaagaatctt cccgtggtgg actacagccg gccccatcag actcgatctc cgattgaacg
    15721 ctgccccaca ggggctatcg tttggatcga tgccaagtcc gggccggtga aaggtccagc
    15781 agctaagaag atcattcgca agggcacgct cagcgacgcc ccgacctaga tgccagcgcg
    15841 aaagcccgat aggtgatttc gatgtcccca cgattcttcg gcaaaaagac ccaggagcac
    15901 ggacgagacg gcgaacgcca gttcaagtat ccaggctctc gcgccgcgct cgacggaaac
    15961 agcgccgtca tcatgtgtga gcgggagtcg accgacgccg cgggcgcgta tccgatcact
    16021 ccctcgactc agatgggtga gttctgggcg gaagccgcag cggctgggca catcaacgtc
    16081 tcgggtcgtc cgctgatctt catcgagccc gaaggcgagc acgctgccgc cgcggtcacc
    16141 gccggaatgt cgatgacggg gatgcgcgcg gcgaacttct cgtccggcca gggcatcgcg
    16201 tacatgcacg agtcgctcta cgcggcaacg ggaaagcggc tgacctacgt cctgaacatc
    16261 ggcgcgcgag cgatgaccaa ggcaacgctg aacgtgcacg ccgggcacga cgactatcac
    16321 tgcgtcgacg acacgggctt ctttcagatg tttgccaaga acgcgcagtc cgcagcggat
    16381 ctgaacatca tcagccatcg gattgcggag ctggcactca cgccggggat catcgctcaa
    16441 gacggctttc tcaccaccca cctgatcgag tcactgatgg tgccggaacg cgagctcatc
    16501 gccgagtacc tcgggcgccc cgatgacatc atcgatacgc ccactccggc tcagaagatc
    16561 atctacggtg aaacgcgtcg ccgcatcccc atgctctggg acgtggacaa cccagtgatg
    16621 tccggactgg tccagaacca ggattcgtac atgcagagcg tggccgctca gcggccgttc
    16681 ttcttcgatc acatccaggc gctgaccgct cgggcgttcg aggagtacta cgagctcacc
    16741 gggcgccgct acgagcgagt gatgacatac cgggcagatg acgcggacta cctcatcgtg
    16801 ggtcagggga gcatggttcc ttcggccgaa gccgtcgtcg actatctccg cgagactcgg
    16861 ggtttgaagg ttggcgtggt ggacatgctc atgttccggc cgtttccgag cgatctgatt
    16921 ggacagatca ttcggggcaa gaaaggcgta gccgtcctcg agcgactcga tcagccgctg
    16981 gcggtagacc ttccgctcat gcgcgagatt cgcgcgaccg tgagcaagtg catggagaac
    17041 gccaaggacc cgaagaatcc ggctttcccc gagctcccga cctatgcctc actccaggat
    17101 gcgccggctc tgtactccgg ctcgttcggc atgggcagcc gtgacctgca accagagggc
    17161 atcgtcggcg cggtcgaaaa tatgctggaa aagggggcgc ggaagaagct cttctacctc
    17221 tccatcgact tcgtccggga cgaggcgatc acaccgaagc aggagatcta ccagcagacg
    17281 atcgaggcgg cctaccccaa cgtgaaaggg ctcgtcgttc gaggttccga gaaccccaac
    17341 ctgatgccca aggacagcat cacggtgcgc tttcactcga tcgggggatg gggtgcgatc
    17401 accacgggca agaacctggc gatgacgctc ttcgatcttc tcggttacca catgaaggcc
    17461 aatcctaaat acggctccga gaagaaaggc caacccacca catactacct ttcggcggcg
    17521 ccggagccga tcaagatcaa ctgcgagtac ttctacgtcg acgtggttct ctcaccggat
    17581 ccaaacgttt tcgggcacac caatgcgctc gcgggcctca aggaaggcgg cgtcttcatc
    17641 atccagagcg ataaggcgtc gtccgaagag atgtgggaaa gcattccgcc catctaccaa
    17701 aagatcatca tcgacaagaa gatcaaggtc tactacctcg acgcgttcaa gatcgcccgg
    17761 gacgaagcca ccgactccga ccttcagctc cggatgcagg gcatcgcgtt ccaaggtgcc
    17821 ttcttcgccg catcaccagt gattaggcag gctgggctga gcgaggagaa gctcctcgac
    17881 gccattcacg accagctcca ggagaagttc ggctcgaagg ggcgtcgagt cgtcgaggac
    17941 aacatccgcg tcgtgaagcg cggtttcgac gagattcgcg agctcgttcc cggcccgatc
    18001 acccggctca aggcagaaag cggacgcgcc gggccgaggc ttccgatgat ggtgaagtct
    18061 ctcccccaga gcaaggctcc catgaccgac attcaccgtt tctgggagca gacgggcagc
    18121 ttctacgcgc gcggcatggg caacgacaac ctcgccgatc cgttcatcgg gctcggcgtc
    18181 atgccgccct cctctgcgtt gtttcgggac atgacgggga tccggttcaa ccatcccgag
    18241 tgggtgcccg agaactgcac cgcttgcggc aattgctaca cggcctgccc cgatacggcg
    18301 ctgccgggcc tggtcaacga agtgggacag gtgctcgaca ccatcgtcgc gcgagtgcgc
    18361 aagaacggcc gcggcgcgga gatcgaacac ttgcccaagg cggccaaagt catggagcgc
    18421 aatctgcacg ccttgttcaa gaaggcagag gagaccgagt ccgtggccct gctcatcgat
    18481 caggccatgg cggagactct gaagcagagc gagctccaag gcggtgagaa ggagaagctc
    18541 agaaaggagc tcgactactt caaggaggag ctcaacgggt tccagttcgc cttgacccgg
    18601 ccgcactaca ccttggccga gaaagatcag gcggggagcg gtgggcttct ctcgattacg
    18661 gtcaaccctt acacctgcaa gggatgcatg gagtgcgtcg aggtctgcaa cgacgacgcg
    18721 ctccggtcgg tgaagcaaac ggacgagtct gtgcagaagc ttcgcgacaa ctgggacctt
    18781 tggacggatc tccccacgac tccgaagaag tacatccgag tcgacgacct cgaagagggg
    18841 atcggcaccc tcgagtcgat cctgctcgag aagtccaact acctgccatt cacgagcggc
    18901 gatggagcct gcctcggttg ttcggagaag accgctctgc atctcttcgt cgccaccgta
    18961 gacgccttga tgcagccgcg gatcgcaaag cacctaagtc atctcaccga gctcatcggc
    19021 cgactgaaaa agcacattca gcttcgactc gcagaagaga tcaacgtcag cgatgccaac
    19081 gtcatggcca aggtcatgga cgacatgggc gatcgggatg tcacgctcgc cgtgatcgcc
    19141 caacggatcg agagcatggg gggcggccag ccaatcgatc aggactggct tcgccgcatg
    19201 accgagctgc tgacgaggct ggaaacgctg aaatggaagt acacggatgg aacgacgggc
    19261 cgcggtcgga ccaacatggg catggtcaac agcacaggtt gcacttctgt ttggggcagc
    19321 acgtacccgt tcaatccgta tccgtttcca tgggctaacc atctctttca ggactcgccg
    19381 tcgatggcca tggggatctt cgaagggcac atggccaaga tggccgacgg cttcaaggcc
    19441 gtccgcaccg ccgagctgga gctcgacaac aagtacaagc ccgcagagca cgacgacttc
    19501 ttcacctatt tcgattggcg ccaattcagc gaccaggagt gggagctctg cccgccagtc
    19561 gtggccgtgg gcggcgacgg cgccatgtac gacatcggat tccagaacct ctctcggctc
    19621 atggcatcgg gcaagccgat caaggtcgtg gtcgtggaca ctcaggtcta ttcgaacacg
    19681 gggggtcagg cctgcacctc tggtttcatc ggccaggttt ccgacatggc ccagtttggc
    19741 aaggccattc agggcaagca ggagcctcgc aaggagatcg gcctcatcgg cctggcgcat
    19801 cgcaacacct acgtgatgca gagcaccatc gcccactcca gccacatgat cgaaggcttc
    19861 atacgcggtc tcaaggcgcg ccggcccgcg cttttcaaca tctacagcac ctgccaaccc
    19921 gagcatggca tcggcgacga gatgagcact gcgcagtcga agctcgcagt cgaatcgcgc
    19981 gcctatccgc tcttccggta cgaccctgat gcgggcaaga ccctccagga gtgtttggac
    20041 ttggagggca acccggatgc cgatcaggac tggcccctct accggatcaa gtaccaggaa
    20101 gacggcgtcg agaaggagat ggagctgcca ctcaccttcg ccgacttcgc gatcaccgag
    20161 acacgattcc gaaagcagtt ccgggtcgcg cctccggata cctggaacga gaacatggtg
    20221 cccctggccg agttcctcga aatggacgag gacgagcgcg aaggcatttt ccccttcgtg
    20281 tggatggtcg acaaggaaga gcacctcacg aggctgctcg tcgctcaacc catcgtggag
    20341 tcttgtgaag accgtctaga tttctggaac atgcttcggt cactcgtcgg tgcggatcaa
    20401 ccgtcgaccg aatcactggt cgaacaggtg cgtcaagacg tcgtcgggaa gctcgtgtcc
    20461 gagctcatgc aaatcgcggc tggatccgga gaagggtttt cggccccggc gaccggcgag
    20521 gttgctgcgg ccgctacgcc cgcagtcgcg cccgccgctg caagcgccgc agcgccagca
    20581 gccggcgcta caggcgacta cttggcgccc tggatcgata cggcaaactg caccgcctgc
    20641 gacgagtgca tccagctcaa ctccaaggtg ttcgtctaca acgccgacaa gaaggcgatc
    20701 atcaagaacg ccgaagctgg ctcgtacaag gacctggtga aggccgcgga gcgctgtacg
    20761 gcccgggtca ttcatccagg cttgccgaga aacagaagcg aaaaaggcat cgagaagtgg
    20821 atcaagcgcg gcgagaagta caactgagaa acgagctcgg acgagaccga tgagactttt
    20881 ggggacgaag accttccggc acggggtgca ccccccggag tcgaaggacg aaacgagcgg
    20941 cctgccgatt cgtcagttcc cgttcgcccc agtgctgatc attccgctgg ttcagcacat
    21001 gggcgcgcca tcgatcccgg tggtagccga gggagatgaa gtatcgcgag gccagcgaat
    21061 cgcgcgcgcc gacggcttca tgtcggtctc gatgcatgcc tccgcatccg gcatcgttcg
    21121 caggattgcc ctcgcgccca gcatcggcgg ccgcatggtg cccgcggtct atttggagcc
    21181 gtttccaggc tccacgcagg aaattgaaga cggcacaccc tgccccctcg aaacctcgac
    21241 gcccgatgag atcatcgcgg caatccaaga cgctggcgtg gtcggcctcg ggggtgctgc
    21301 attcccgacc cacgtcaagc tgcaggtccc ggaaggcaag ttcgtcgaca cactgatcat
    21361 caacggcgtg gagtgcgagc cgtacctcac caccgatcat cgggtgatgt tggagcagag
    21421 cgacgacgtg ttcatgggca tccgatacct tctcaaggtc tccggggcga agaaggtgat
    21481 cgttgccgtc gaagcgaaca aacccgacgc ggccgagcat ctacgaaaga acttcccgag
    21541 cgatctagac gggtcggtcg aggtgctcca agtgaagtat cctcagggag ccgagaagat
    21601 gttgttcgcc gcgcttctcg ggcgggaggt gccctccggg ggactgcctc tggacatcca
    21661 tgccctctgc tgcaacgtgg ccaccaccgc cgagatcggt cgtctcttgc ctctcgggcg
    21721 gggcattcag gaacgggtca tcaccgtgac cggccctgcg gtgaagcgca aaggcaacta
    21781 ccgcatcccc atcgggaccc cgctccgttt cttgctcgag caggtgggcg tcgaagagga
    21841 cgtgactcgc gtgttcctcg gaggcccgat gatgggcgca gccgcctcca gcttggatat
    21901 ctcgatcacc aagggaacat ccggagtgac cgccttcacg gactcggccg cggagaggac
    21961 ccatagcccc gtgtacccgt gcatccgatg cggctactgc gtcgatgcct gccccatctt
    22021 tctagatccg tctctgctgg gaaggctggc cgccaacgag gagttctccg ccatggcgga
    22081 gcggcacaat ctcatggact gcttcgaatg cggctcctgc tccttcgttt gtccgtcgca
    22141 catccccctc gttcagcgct ttcgcgcagc caagaccgcg gtgcgcaaag ccaacgcgcc
    22201 caaggcgggg gcagcctgat gacgacaccc ggcaagacgc tggagatccg cacgtcgccg
    22261 cacatcctca gcggcaacag cgtcgacacc atcatgttca acgtggtcct cgcgctgctg
    22321 ccggttacgg cattcgccgt ttatgcattc ggtctggccg gcttgctcac gctctccatg
    22381 gctctgacat cctgcgtgct caccgaacac ctgatctgcc gtgcaacggg aaagcccagc
    22441 acagtcggcg actggtcggt gaccatcacc ggcttgctct atggcctcac ccttccgccc
    22501 gggttgccac tctggatggt ggctgtcggg ggcctgatct gcgtcgccat gggcaaggca
    22561 ttattcgggg gtctcggtta caacacattc aatccagcgc tggtcggacg agcggtgttg
    22621 caggccgcct ttccagtggc gatgaccact tggatcccag gcttcatggc aggacgcttc
    22681 tcctcgctgc cttcgtccac gctgaccttg ccgcttaccg agccggccgt cgatgcaatc
    22741 accagcgcta cccccctcgc gttgtggaag ttcagccatg aggcgactcc aatctccgag
    22801 ctctccttgg gcttcgtcag cggttcggcg ggcgagacct cgagcgtgct cattctattg
    22861 ggtggcctct atctcatcgc ccggaacatg ataaac
//
LOCUS       DASRZF010000117         2317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4550902, whole genome shotgun sequence.
ACCESSION   DASRZF010000117 DASRZF010000000
VERSION     DASRZF010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4550902"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="VFG22_05485"
     CDS             <1..684
                     /locus_tag="VFG22_05485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HET6415726.1"
                     /translation="EERFGKTMRAHFRWLESEVMANLKSLPGWMISQKAGDGSDLFDD
                     LEARRRLRAAVSPIYRAAMARGIDTMALELALRTSVSLLDPRIVARLTELVHKIVDVD
                     ARIKQAIREALAEGIQEGESTAEMAERIRDIFDMSRTRANTIARTEVGSAFSGGRHVQ
                     MIDAGIHMQEWLSARDDAVRDSHKIDGEKIAIGETFSNGLRYPNDPNGKADEVINCRC
                     VALPVVSEE"
     gene            697..1383
                     /locus_tag="VFG22_05490"
     CDS             697..1383
                     /locus_tag="VFG22_05490"
                     /inference="COORDINATES: protein motif:HMM:NF016471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HET6415727.1"
                     /translation="MEPCVPNVMRAAVEQIVKAKNPTTRELLHLITTANPDRAGDVVE
                     AGGAQVDSFMRNPVVMADHDYSFEKIIGRAMSLEVRDGGIYARTQFRSTPLGEAAYNL
                     AAEGIGGWSIGFRPIEYDNIKGEDGRTRGYRFKKWELLEYSIVAIPMNADAVSSAIQR
                     GLVGSDIANVFFRMIKAEAPVLPEGTNPVAPPKVIRVSAASLREIQNIKRSIDRADAA
                     LRVTETARSL"
     gene            1385..>2317
                     /locus_tag="VFG22_05495"
     CDS             1385..>2317
                     /locus_tag="VFG22_05495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HET6415728.1"
                     /translation="MAEDTALEAMEGAVELQPLVDGIKHVADFSTKNTKTLDEKIEKL
                     ALKSTREVGDLKEEFNKSYENLLARVNHGIDLNVMKLSTLGVISSGDGLKDVIPKSIK
                     DHAGSFEAAARSVPSGSAMSDPTTLMAIHTWFTRTAKLGLREFMANSHQNATEKDKAY
                     AALQEKHFGSNWEAITKADYATGTTPGTGSTGGTLVPTIVENDIIRQIKDAGKLFPLA
                     RQVQMTSKIHNMPSEATAVTVGWALEAGTLSQGEGTLAQKALNAFKIYGRAKMSIELV
                     EDSNPGLLAYLLEVFTEKMAGQLDRQLVLGDGSNP"
BASE COUNT          484 a          723 c          750 g          360 t
ORIGIN      
        1 gaggagcgtt tcggcaagac gatgcgcgct cacttccggt ggctcgagag cgaggtcatg
       61 gccaacctca agtctctccc cggatggatg atctcgcaga aggccgggga cggttccgac
      121 ctgttcgacg acctcgaggc aaggcgcagg ctgcgcgcgg ccgtgtctcc gatctaccgc
      181 gccgcgatgg cccgcggcat cgacacgatg gcgctcgagc tggccctgcg gacgtccgtc
      241 tcgctgctcg accctcgcat cgtcgcgcgc ctcaccgagc tcgtccacaa gatcgttgac
      301 gttgacgcga ggatcaagca ggcgatccgc gaggcgctgg ccgaggggat tcaagagggc
      361 gaatcgacgg ccgagatggc ggagcgcatc cgcgacatct tcgatatgtc ccgcacgcgg
      421 gcgaacacca ttgcgaggac cgaggtcggg tccgcgttct ccggtggccg gcacgtccag
      481 atgatcgacg cggggattca catgcaggag tggttgtcgg cgcgagacga cgcggtgcgc
      541 gactcgcaca agatcgacgg cgagaagatc gccatcgggg agacgttctc caacgggctc
      601 aggtatccca acgacccgaa cgggaaagcc gacgaggtca tcaactgccg gtgtgtagcg
      661 ttgccggtag tgagcgagga atagatggac gaaaacatgg aaccctgcgt cccgaacgtc
      721 atgcgtgcgg ccgtcgagca gatcgtcaag gctaagaacc cgacgacgcg ggagctactc
      781 cacctcatca ccaccgcgaa ccccgaccgc gccggcgacg tcgtggaggc tggcggggct
      841 caggtggact ccttcatgcg aaacccggtg gtcatggccg accacgacta cagtttcgag
      901 aagatcatcg gtcgcgccat gtcgctcgag gttcgagacg gcgggatcta cgcgcgaacg
      961 cagtttcgca gcaccccgct cggcgaggct gcgtacaacc tcgcggccga gggcatcggc
     1021 gggtggtcga tcggcttccg gcccatcgag tacgacaaca tcaaggggga ggacgggcgc
     1081 acccgaggct accgtttcaa gaagtgggag ctgctcgagt actcgatcgt cgccatcccc
     1141 atgaacgccg acgcggtgtc gagcgcgatt cagcgcggcc tcgtcggttc cgacatcgcc
     1201 aacgtatttt tccggatgat caaggccgaa gcgccggtgc tgcccgaggg tacgaaccct
     1261 gttgcgcctc cgaaagtcat ccgggtgtcc gccgcatcgt tgcgggagat ccagaacatc
     1321 aagcggtcga tcgaccgcgc agacgcggcg cttcgggtca ccgaaaccgc gaggagtctc
     1381 taaaatggcc gaagatactg ccttggaagc gatggagggg gcggtcgagt tgcagcccct
     1441 ggttgacggc atcaagcacg tcgctgactt cagcacaaag aacacgaaga cgctcgacga
     1501 gaagatcgag aaactggctc tcaagagcac cagggaggtc ggggacctca aagaagagtt
     1561 caacaagtcg tacgagaacc tactcgcgcg ggtcaaccac ggcatcgacc tcaacgtgat
     1621 gaagctctcg acgctcgggg tgatctcgag cggcgacggc ctcaaggacg tcatccccaa
     1681 gtcgatcaag gaccacgccg gttcgttcga ggccgcggct cgcagcgtcc cgagtggatc
     1741 ggccatgtcc gatccgacga ccctcatggc cattcacacg tggttcacga ggactgcgaa
     1801 actcggcctg cgcgagttca tggcgaacag tcaccagaac gccaccgaga aggacaaggc
     1861 gtacgcggct ctccaagaga agcacttcgg gtccaactgg gaagcgatca cgaaggccga
     1921 ctacgctacg ggcaccaccc ctggtacagg ctcgaccggc ggcacgttgg tcccgacgat
     1981 cgtcgagaac gacatcatcc gccagatcaa ggacgctggt aagctcttcc cgctggcccg
     2041 ccaggtccag atgacatcga agatccacaa catgccgtca gaggcgacgg ccgtgacggt
     2101 cgggtgggcg ctcgaggctg gcacgctctc acagggcgag ggcacgctcg ctcagaaggc
     2161 cctcaacgcg ttcaagatct acgggcgcgc gaagatgagc atcgagctcg tcgaagacag
     2221 caaccccggt ctgctcgcct acctgctcga ggtcttcacc gagaagatgg cgggtcaact
     2281 ggaccgtcag cttgtgctag gtgacggctc gaaccct
//
LOCUS       DASRZF010000118         3183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152 k141_80031,
            whole genome shotgun sequence.
ACCESSION   DASRZF010000118 DASRZF010000000
VERSION     DASRZF010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80031"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VFG22_05500"
     CDS             <1..560
                     /locus_tag="VFG22_05500"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6415729.1"
                     /translation="TLLNIIGLLDRPTQGRMLIEGRTVEELDERALTDLRGEKLGFIF
                     QSHHLLPGLSVIENVMLPSAAAEGAFRATMKPRARALLDSMGLGKWAEAWPRQLSGGM
                     QQRVAVARALMNDPPLVLADEPTGNLDTQTSDQVMELLLERNAKTATAFLIVTHDRHL
                     AERCERVIELVDGVIVFDGPPHQRA"
     gene            complement(685..1086)
                     /locus_tag="VFG22_05505"
     CDS             complement(685..1086)
                     /locus_tag="VFG22_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415730.1"
                     /translation="MGKLFPITVVAFAAAAFFVTDVKADNSAGVCNSIVASGMRGAQT
                     CENMSCAPGDDACMDVTNVFIEFVTTDGCVDLFANGELKGLPGNASIQPNGPNAGGAK
                     HVQELICGAVRDCGLCPSALLVGICVLPCED"
     gene            complement(1356..1685)
                     /locus_tag="VFG22_05510"
     CDS             complement(1356..1685)
                     /locus_tag="VFG22_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415731.1"
                     /translation="MSNECALTATMHASASEALQAVERAFVKAHVFLTEAHQLGGLAL
                     VAHWDMEGDHRQLLVDALLEESVNLDESAFETLQEPWEGVLTGSLHITLEHEGRDSKV
                     DVPAVPG"
     gene            complement(1691..2395)
                     /locus_tag="VFG22_05515"
     CDS             complement(1691..2395)
                     /locus_tag="VFG22_05515"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET6415732.1"
                     /translation="MGTPRFKLYHYPATRSARVKWMLHEVLDDDFEVEIVPVYERKQY
                     EPEYLSKNPNHNVPTLEIKMPDGRSMHMIESGAMVTFLADAFPEKGLAPAPSPLSLER
                     ADYLQMLHFGASWMDMMLWQIRVHEHLLPKEQQDPRTIQRYREKFTNEAEPQIRKRFE
                     RHPFICGDAFSAADCVMGHTVSWARGYGLCRDEIFRGYISRLSKRPAFVKAFSDVGAF
                     QIKAPEGSSLLERFTG"
     gene            complement(2406..>3183)
                     /locus_tag="VFG22_05520"
     CDS             complement(2406..>3183)
                     /locus_tag="VFG22_05520"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6415733.1"
                     /translation="ENFTVLAPDLLGHGESDKTKGDYSLGSMASTLRDLIVALGYKRA
                     TVVGQSYGGGIAMQFAYQYPERCERLVLVDAGGLGKEVNRLLRMLTLPGSEAVLLLGC
                     APPVRRVVEAIGRVAFRKKLQNDTVIPELWRSYSSLGDVEARRAFLRTLRAVIDPRGQ
                     AVSANDKLYLAAGIPTLIIWGENDRIIPVRHAYAAHAAIPGSWLEIIEGVGHYSHVEA
                     PERFVTALTEFIESTRPARIKVSQKQILGPPNTTRSGSES"
BASE COUNT          628 a         1053 c          908 g          594 t
ORIGIN      
        1 gcactttgct caacatcatc ggccttctcg atcgccccac ccagggacgc atgctgatcg
       61 aggggcggac cgtcgaggag ctcgacgagc gcgcgctcac cgatcttcgc ggcgagaagc
      121 ttggattcat cttccagtcg catcatctct tgccgggcct aagcgtcatc gagaacgtca
      181 tgctcccgag cgccgccgcc gagggtgcgt tccgcgcgac catgaagccc cgcgcgcgcg
      241 cgctcctcga ctccatggga ctcggaaaat gggccgaggc ctggccgcgt cagctttcgg
      301 gcggcatgca gcagcgcgtc gccgtcgcca gagcgttgat gaatgatccg cctctcgtcc
      361 tcgcagacga gcccaccggc aacctcgaca cccagacctc cgatcaggtc atggagcttc
      421 tcctcgaacg aaacgccaaa acggccacag cgttcctgat cgtcacacac gaccgacacc
      481 tcgcggaacg atgtgaacgg gtcatcgagc tggtcgatgg cgtcatcgtc ttcgacgggc
      541 cgcctcacca gcgagcctga tctcctcacg aacctcagcc accaggcacg gcaggcacat
      601 ccaccttgcc gtaaccgcag ccgcagagca caagaaagcc ccggccgaaa ccgaggcttt
      661 ccgttcgctt tttcgtttct ggcgctagtc ctcgcaggga agcacgcaga ttccaaccaa
      721 gagcgcggac ggacaaaggc cgcagtctct aaccgctcca cagatgagct cttgaacgtg
      781 cttggcgcca ccggcgttcg ggccgttggg ctggatcgaa gcgttgcccg gtagaccctt
      841 gagctcgccg ttggcgaaca agtccacaca gccgtcggtc gtgacgaact cgatgaacac
      901 attggtcaca tccatgcacg cgtcgtcgcc cggcgcgcaa ctcatgttct cgcatgtttg
      961 cgctccacgc atgccgctcg ccacgatcga gttacagacg cctgcactgt tgtccgcctt
     1021 gacgtcggtg acgaagaatg ccgccgcggc gaatgcgacg acggtgattg gaaatagttt
     1081 gcccatgata gcctccttca gaaagcccgc ttggaaggag tgaagcaaca cgcgacacca
     1141 acccccaaga tctgcgaggg tctcgcgagc ttcgggacgc cgtctatacg taattattcc
     1201 acgcgcaaaa acgttctgca tgaacgacat cgcgtgaaca ggatgcacgt cgctaccttc
     1261 cagcacacgg tgacacatgg gcgtagtccc tatctaggcg tcccgatttg cgagttcgag
     1321 gacacgcgca aaacagcaga cgggcgcgcc gccgatcacc caggcaccgc aggcacatcc
     1381 accttgctgt ccctgccttc gtgctcgagc gtgatgtgaa gcgagcccgt gagcactcct
     1441 tcccaaggtt cttgcagtgt ctcgaacgcg ctctcatcga ggttcaccga ctcttcgagc
     1501 agtgcgtcga ccaagagctg gcgatgatca ccttccatgt cccaatgcgc gaccaaggcc
     1561 agcccaccga gctgatgggc ttcggtcaag aacacatgcg ccttcacaaa tgcgcgctcc
     1621 accgcctgca acgcctcgga ggcgctcgca tgcatagtgg ccgtaagcgc acattcatta
     1681 gacacggcgt ctacccagta aaccgctcca agagcgagga tccctccggc gctttgattt
     1741 gaaaggcgcc gacgtcagaa aacgccttaa caaacgccgg cctttttgac agtctcgaaa
     1801 tgtagccgcg gaagatctcg tcacggcaga gcccgtagcc tctcgcccag ctcacggtgt
     1861 gccccatgac gcagtctgct gcgctaaacg catcaccgca gatgaatggg tggcgctcga
     1921 agcgctttct gatctgcggc tcggcctcgt tcgtgaactt ctcgcgatag cgctgaatcg
     1981 tgcgcgggtc ctgctgctcc ttcggaagaa gatgctcatg cacccgaatc tgccaaagca
     2041 tcatgtccat ccaggaggca ccgaagtgga gcatctgtag ataatccgcg cgttcgagtg
     2101 agagcgggct cggcgccggt gccagcccct tctccggaaa cgcgtcagcc agaaacgtca
     2161 ccatcgcgcc gctctcgatc atgtgcatcg aacggccgtc cggcatcttg atctccaacg
     2221 tgggcacgtt gtggtttggg ttcttgctga ggtactcggg ctcgtactgc tttctctcgt
     2281 acaccggtac gatctcgact tcgaagtcgt cgtcgagcac ttcgtgcagc atccacttca
     2341 cccgcgcgct tcgtgtcgcc gggtagtgat agagcttgaa cctgggtgta cccatcgatg
     2401 acctcctatg actcagatcc ggaacgcgtc gtattgggcg gccccaagat ttgcttctgc
     2461 gacactttga tccgcgccgg ccgcgtggac tcgatgaact cggtgagcgc ggtgacgaag
     2521 cgttcgggcg cttcgacgtg ggagtagtgg cccaccccct cgatgatctc gagccaactt
     2581 ccggggatcg ccgcgtgcgc ggcataggcg tgccgtaccg gaatgatccg atcattctcg
     2641 ccccagatga tgagcgtcgg gattcccgcg gccaggtaaa gcttgtcatt cgcgctcacc
     2701 gcttgacccc gcgggtcgat cactgcgcgc aatgtgcgca gaaacgcgcg gcgcgcctct
     2761 acgtccccaa gggacgagta gctccgccag agctcgggaa ttaccgtgtc gttctggagc
     2821 ttctttcgga acgcgacccg gccaatcgcc tcgaccacgc ggcggacggg cggtgcgcag
     2881 cccaagagca aaacggcttc ggacccgggt aaggtcagca tccgaaggag ccggttgacc
     2941 tccttaccca gaccacctgc atcgacgagc accagccgct cgcagcgctc ggggtactga
     3001 tacgcgaact gcatcgcgat cccaccgcca tacgactgcc ccaccaccgt ggcgcgcttg
     3061 tatcccaggg ccacgatcag atcgcggagc gtagaagcca tggaaccgag cgaatagtcg
     3121 cccttggttt tgtccgactc gccgtggccc aagaggtccg gggccagaac ggtgaagttc
     3181 tcg
//
LOCUS       DASRZF010000119         7036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1112938, whole genome shotgun sequence.
ACCESSION   DASRZF010000119 DASRZF010000000
VERSION     DASRZF010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 7036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7036
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112938"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /gene="ruvA"
                     /locus_tag="VFG22_05525"
     CDS             <1..572
                     /gene="ruvA"
                     /locus_tag="VFG22_05525"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HET6415734.1"
                     /translation="ADGSVIVDVNGVGYEVFLPPGAVGRIGSEPGEAVTLEIHTHVRE
                     DAMTLFGFATPEDRAAFRALLKVSSIGPKLALAIIGVMNASELQDAIARQDKAAFKGI
                     TGVGKRTVERILVDLQDKLDFAPTGRTGVRLRAVPSTGSSIEDRVVGALVQMGYKRSE
                     AEAAVGNIMGDSSDENVEGLLRAALSALA"
     gene            578..1606
                     /gene="ruvB"
                     /locus_tag="VFG22_05530"
     CDS             578..1606
                     /gene="ruvB"
                     /locus_tag="VFG22_05530"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF000868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HET6415735.1"
                     /translation="MEARANQSLITDEQPEDRALDRSLRPAQFDDFVGQDRIKDNLRV
                     FVEAARRRGEPIDHTLFCGPPGLGKTTLALILAGERGVTLHSASGPAIEHKGQLAALL
                     TKLEPNDVLFIDEIHRLNATVEENLYTAVEDFRIDIVNGDGPYAQTLQLPLNPFTLVG
                     ATTRTGLLTNPMRSRFGYVARLDYYPDDALRSIIERSAKLLKLAVSTEAAQELARRSR
                     GTPRIANRLLRRARDFAEILSDGKVDIRTAHEALDRLDVDRAGLDQMDRRYLTVIIDH
                     YDGGPVGIETLAAALSEPRDTLEDVYEPYLLQKGLLARTARGRVATRHAFEHLGRKQP
                     SGSQSGLF"
     gene            1665..3209
                     /locus_tag="VFG22_05535"
     CDS             1665..3209
                     /locus_tag="VFG22_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415736.1"
                     /translation="MSHRPDAAKEGLLFAAERWTPADAELWGRWREGLSKRQREHALL
                     PLQAALSGLVAFRHLENHPLSESVSDFRPHLHAVHVSSAWALELVARLGGGDTEDRAL
                     RPVGERLGEPELSLKALERSLRDAHRVSERLLELPLVDAGAFQASSDLFLRDLGRNAF
                     FHPPEPLEFSNVAELVRPAGLTPELESWKSEAAKMTTVIAFLALLRAHRYLGIADRQI
                     FEDEGLYRAHIVLASVRRELRTLTRFLLVQGVEAFADELEARLLSLDAHHITGARAEI
                     TRTSSELRALRESVETLAMGIHSKVRSALDNALPEPDCEPGLALPAERMRSGIREVRE
                     TVKDAAKQLRAILGPVRNEPKSERVKKNVQQDIWAFRFILRAFIAKAAVAPSNVNRWS
                     DLDSLKFVAEFVRHFRVFGPRLSEATEYERRGPLARAVGALAQHGAIDASKLALAIEE
                     CAGFLEHLHEALAETPHSLLVTFDKKKAAAELRGYLLAARDRGSPDRVAAGAFGVMDS
                     NRAKAG"
     gene            complement(3206..4210)
                     /locus_tag="VFG22_05540"
     CDS             complement(3206..4210)
                     /locus_tag="VFG22_05540"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HET6415737.1"
                     /translation="MRALGLLIAALLALGAPEVARAHPLAPSVLSFEQHADGTVTMQW
                     RAPVKRPTGQSLRPRIPESCEPLAPAELRRTEDEIAIVETVELRCEPPDMLGTVIEVD
                     GFADSSINVVLRLVREDGTMHHSILDAGTPSLTIARPEDESHSFFEYLALGVEHLLTG
                     WDHLTFVLGLMLLFGWHRRLIAAVTAFTLGHSLTLALSVLGIVSLPMALVEASIAMTI
                     VVLALELQSGQHGPVWRHPWTLPGALGLLHGLGFASVLFDAGLPADEIPVALLGFNLG
                     LELGQLGVIALAGACFAAIRSLLPANWQADRGIAAYAIGSLAAYWVIERTLAAFGFVV
                     "
     gene            complement(4210..5088)
                     /locus_tag="VFG22_05545"
     CDS             complement(4210..5088)
                     /locus_tag="VFG22_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET6415738.1"
                     /translation="MLRSLIHFFLIGGLLFSAKALYKGRRVEGLEITVSVPVKSTPSE
                     IETAVEEAILFNEAKRYGWDKRDPIVYTLLVRNMRFIEPDSTDDDLTLYKRAIEMNMQ
                     AHDPLVRARMLYRAREALAHVPEERALSREELEAHRSSHPERFERPGRFRFQQVFLSR
                     AKRGASLGADASTMRDRLSELGDQAPTGLGDPLPGLRLERSATLIEIRNDYGYELASL
                     VEEAVLGSWRGPVSSVYGLHFVKVLEQRPAYVPVLDAIVAEVRADRMREIRAELSEER
                     MAALRDAYTVKIERVP"
     gene            complement(5088..>7036)
                     /locus_tag="VFG22_05550"
     CDS             complement(5088..>7036)
                     /locus_tag="VFG22_05550"
                     /inference="COORDINATES: protein motif:HMM:NF023649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HET6415739.1"
                     /translation="DFDPLRQVYWGDTHIHTELSFDANMQGTRTSQDDAYAFARGATI
                     PLQPYTADGSPTRSATIDRPLDFVMLSDHAEFLGTLKVCNDPTSPGYNADQCVEYRAA
                     MQFDATPAEVTFIFVEINGLTIFPPDEAHYPALCGPMNESCLQAGMDVWSEIVSKAEG
                     AYDRSDSCEFTSFPGYEWSGGPMAKNLHRNVMFKNEKVTDLPYSYFDAPYAEGLWAHL
                     REGCLDAGNGCDVLTIPHNSNLSEGIYFQDKMANGEPFTAEYAALRNAMEPVIEIYQH
                     KGASECIPGAASSDELCGFESIPFSNLATTDQERITPPDPKGFLRHAYGEGLKLEANL
                     GTNPFQYGITAATDTHISAPGFVAENAFKGHGGAGQPNRFLPPPQGFPDNEYLSPGGL
                     TAVWAEENAREAIFSAMRRKETFGTSGPRIVVRMFGGWDYPTDLCNAEDLAAQADAAG
                     VPMGGTLASAPDSVSPMFVVSARQDPIGAPLQRIQIVKGWLEGEDYQVQVYDIAGDAN
                     NGASVDLGTCERQGEGFGDLCSVWQDPDFDPSQRAYYYARVLENPSCRWTSWQCSTAE
                     YDCNDWNFEVCAALKDDPNGAQSNYTCDCCDPATGLNEGFCESVDCSDPGLLSEPEER
                     CCHQVEPSIQERAWTSPIWYQPPG"
BASE COUNT         1348 a         2170 c         2264 g         1254 t
ORIGIN      
        1 aggccgatgg ctcggtgatc gtggatgtga atggtgtggg gtacgaggtg tttctgccgc
       61 ctggggccgt agggcgcatc ggctcggagc caggcgaggc ggttacgttg gagatccaca
      121 cgcacgttcg agaagacgcg atgacgctgt tcggtttcgc cacgcccgaa gatcgcgccg
      181 cattccgagc tcttctcaag gtgagcagca tcgggccgaa gctcgcactc gcgatcatcg
      241 gcgtgatgaa cgcgagtgag ttacaggatg cgatcgcgcg ccaggacaaa gccgcattca
      301 aaggaattac cggggttggc aagaggaccg tcgagcgaat tctcgtcgat ctacaagaca
      361 agctcgactt cgccccgacc ggcaggactg gggtgcgctt gcgcgcggtt ccgagcaccg
      421 gaagctcgat cgaagaccga gtcgttggtg ccctggtgca gatgggatac aaacgaagtg
      481 aagcggaggc ggcggtcgga aacatcatgg gtgattcgag cgacgagaac gtggaagggc
      541 tcttgcgcgc tgcactgtcg gccttggcat gactgaaatg gaagccagag cgaaccagag
      601 cttgatcacc gacgaacagc cagaggaccg agcgctcgat cgaagcctcc gcccggcgca
      661 gttcgacgat ttcgtcggcc aggaccgaat caaggacaac ctccgggtct tcgtcgaagc
      721 cgcgcggcgg cggggcgagc cgatcgacca cacgctgttc tgcgggccgc cggggctcgg
      781 caagaccact ctagcgctga ttttggcggg cgaacgcggt gtgacactgc actcggcgtc
      841 ggggcctgcg atcgagcaca agggacagct cgcggcgctc ctcacgaagc tcgagcccaa
      901 tgacgtgctg ttcatcgacg aaatccatcg cctcaacgca acggtcgaag aaaacctcta
      961 cacggccgtc gaggacttcc ggatcgacat cgtcaacggg gacggaccct atgcgcaaac
     1021 actgcagctt ccgttgaacc ccttcacgct ggtcggggcg accacgcgaa ccggactcct
     1081 gaccaatccg atgcgctcgc gctttgggta cgtcgcacgt ctcgactact acccggacga
     1141 cgccttgcgc tcgatcatcg aacggagcgc aaagctccta aagctcgcgg tctctacaga
     1201 agccgctcag gagttggccc ggcgttcgcg gggcacccct cggatcgcca atcgcttgct
     1261 gaggcgcgcc cgggacttcg ccgagatctt gtcggatggc aaagtcgata tcagaaccgc
     1321 gcacgaggcc ctcgatcggc tcgacgtgga tcgcgcgggc ctcgatcaaa tggacaggcg
     1381 atatctcacc gtgatcatcg accactacga tggcggtccg gtgggaatcg aaaccctggc
     1441 ggctgccctc agcgagcccc gggacacgct ggaagacgtg tacgagccct atctcctaca
     1501 aaagggcttg ctggcgcgca ccgccagggg gcgggtggcg acgcggcatg cgttcgagca
     1561 cctaggccgc aagcagccga gcggctcgca atccggcctg ttttgaagca cttacactga
     1621 gaatccccgt tcctgtcggg gcgctgttag agtcgacccc ccatatgagc caccgtcccg
     1681 acgcggccaa agagggcctc cttttcgcgg ctgaacgctg gacgcctgcc gacgcggagc
     1741 tctggggccg ctggagggaa gggcttagca agaggcagcg cgagcatgcc ttgctccctc
     1801 tgcaggcggc cctttctggg ctcgttgcgt ttcgccatct cgagaaccac ccgctctcgg
     1861 agtcggtcag cgacttccgt cctcatctac atgcggtgca cgtctcctct gcatgggccc
     1921 tcgagctcgt cgctcgactc ggcggtggcg atacggagga ccgagcgctg cgtccggtcg
     1981 gcgagcgtct gggcgagcct gagctctccc tgaaagcact cgaacgttcg cttcgcgatg
     2041 cacaccgggt gagtgagcgg cttctggagc ttccgctcgt cgatgccggc gccttccagg
     2101 ctagctcgga tctgtttctt cgcgacctag gtcgaaacgc attctttcat ccgcccgaac
     2161 cgctcgagtt ttcgaatgtc gctgagctcg tgcgccccgc cggactgacg ccggagctcg
     2221 aatcctggaa gagcgaggca gccaagatga cgaccgtcat cgcatttctg gctctgctcc
     2281 gcgcgcatcg atatctgggg atcgcggatc ggcagatctt cgaggacgaa gggctctatc
     2341 gcgcacacat cgtcttggcc tccgttcgaa gagagcttcg cacactcacg cgattcctct
     2401 tggtgcaagg cgtcgaagcc tttgcggacg agctcgaagc aaggttgctc tccctcgatg
     2461 cacaccacat caccggcgcc cgggcagaga tcactaggac atcgagcgag ttgagggcgc
     2521 tccgggaatc ggtggaaaca ctggcgatgg gcatccattc gaaggttcga tccgctctcg
     2581 ataacgcgct tccggaaccg gactgcgaac ctggccttgc ccttccggcc gagcggatgc
     2641 gaagcggaat tcgcgaggtg cgggaaaccg tgaaggacgc cgccaagcag ctccgggcca
     2701 ttctcggacc cgttcgcaac gaaccgaaaa gcgagcgtgt aaaaaagaac gtgcagcagg
     2761 acatctgggc gttccgtttc attttgcggg ccttcattgc gaaagccgcc gtggcgccct
     2821 cgaatgtcaa ccgctggagc gacctggata gcttgaagtt cgtcgccgag ttcgttcgcc
     2881 actttcgcgt cttcggccct cggctcagcg aagccactga gtatgaacgt cgaggtccgc
     2941 tggcgcgcgc ggtgggcgcc ctcgcccaac acggcgcgat cgacgcatcc aagctcgctc
     3001 ttgccatcga agagtgtgcc gggtttctcg aacatctcca tgaagccctg gcagaaacgc
     3061 cacattcgct actcgttacg ttcgacaaga agaaagctgc ggccgagctt cgcgggtatc
     3121 tcttggccgc gagagaccgt ggctcacccg accgtgtcgc tgccggtgca ttcggcgtga
     3181 tggattccaa tcgcgcgaaa gctggctaga ccacgaatcc gaaggcagcg agggttcgtt
     3241 cgatcaccca atacgcggca agggacccga tcgcgtacgc agcgatgccg cggtcggctt
     3301 gccagttggc cgggagcagc gaccgaatcg cagcgaagca agctccggcg agcgcgatca
     3361 cgccgagctg gccgagctcg aggcccagat tgaaaccgag aagcgcgacg gggatctcat
     3421 cggctggaag ccccgcatcg aagagcaccg aagcgaaacc aaggccgtga agaagaccga
     3481 gggcgcccgg aagcgtccag ggatgcctcc agacgggacc gtgctgcccc gactgaagct
     3541 cgagcgcgag gacgacgatc gtcatcgcga tcgaggcctc cacgagcgcc atgggcagcg
     3601 agacgatgcc aaggaccgac agggccaagg tgaggctgtg ccccagggtg aacgccgtca
     3661 ccgcggcgat caggcgtcgg tgccaaccga acaggagcat gagacccagc acgaaggtga
     3721 gatgatccca gcccgtgaga aggtgctcga cgccgagcgc caagtactcg aagaacgaat
     3781 gagactcgtc ttccggccta gcgatcgtga gcgacggagt accggcgtcc aagatgctgt
     3841 ggtgcatggt tccatcttca cggaccaggc ggagcacgac gttgatgctg gagtcggcga
     3901 aaccgtcgac ctcgatcacg gttccgagca tgtccggggg ctcacaacgg agctcgaccg
     3961 tctcgacgat ggcgatctcg tcttcggttc tgcgcagctc ggcgggcgcc aagggttcgc
     4021 aggattcggg tatccgcggt cgaagcgact gcccagtcgg ccgcttcacc ggcgcgcgcc
     4081 attgcatcgt cacggtaccg tcagcatgct gctcgaacga gagcaccgag ggcgccaagg
     4141 gatgcgcgcg ggcgacctcg ggtgcgccaa gagcgagcag cgcagcgatc agcaggccca
     4201 gggcacgcat cagggaaccc tctcgatctt cacggtgtac gcatcccgga gcgccgccat
     4261 tcgctcctcg ctgagctccg cgcggatctc gcgcattcgg tcggccctga cctcggcgac
     4321 gatcgcatcg agcacgggca cgtaggcggg tcgctgctcg aggactttca cgaaatggag
     4381 cccgtagacc gaagaaaccg ggcctctcca ggaaccgaga accgcctctt cgaccagcga
     4441 ggcgagctca tagccgtagt cgtttcgaat ctcgatgagg gtggcggaac gctcgagacg
     4501 aagcccgggc aggggatctc cgagaccggt cggtgcctga tctcccagct ccgacaagcg
     4561 atcgcgcatc gtactcgcat cggcgccgag agacgcgcct cgttttgcgc gactgagaaa
     4621 gacctgctga aagcgaaacc tgcccggacg ttcgaagcgc tcaggatgcg aggaacgatg
     4681 tgcctcgagc tcctcgcggg aaagagctcg ttcctccggc acgtgcgcca aagcctcgcg
     4741 agcccgatac agcatgcgcg cgcggaccag ggggtcgtgg gcctgcatgt tcatctcgat
     4801 ggcccgcttg tagagggtca gatcgtcgtc cgtggaatcc ggctcgatga aacgcatgtt
     4861 ccggaccaga agcgtgtaca cgatcggatc acgcttgtcc cagccgtagc gctttgcctc
     4921 gttgaatagg atcgcttcct caacggctgt ttcgatctcc gagggcgtcg atttcacagg
     4981 cacgctgact gtgatctcga gcccttcgac gcggcgcccc ttgtagagcg ccttcgcact
     5041 gaagagcaga ccgccgatca gaaaaaaatg gatgagcgag cgaagcatca acccggtggt
     5101 tgataccaga tcggcgaggt ccaggcgcgc tcctggatgg aaggctcgac ttggtggcag
     5161 cagcgttctt cgggttcgga tagcaggccg ggatcgctgc aatcgaccga ttcgcagaag
     5221 ccctcgttga gcccggtggc tggatcgcag cagtcgcaag tgtagttgct ctgggcgcca
     5281 tttggatcgt ccttcaaggc cgcgcagact tcgaagttcc aatcgttgca gtcgtactcg
     5341 gcagtcgaac attgccagct cgtccagcgg cagctcggat tctcgagcac gcgagcgtag
     5401 tagtaggccc gctgactggg atcgaagtct ggatcctgcc agacggagca gaggtcgccg
     5461 aagccctcgc cttggcgctc gcaggttccg aggtcgacgc tcgcgccgtt gttggcgtcg
     5521 ccggcgatgt cgtagacctg gacctggtag tcctcaccct cgagccatcc tttcacgatc
     5581 tggatgcgtt gaagcggcgc cccgatggga tcctgcctcg ccgagaccac gaacatgggc
     5641 gacacggaat cgggcgcgct tgcaagcgtg ccacccatgg gaacgcccgc tgcatcggcc
     5701 tgcgcggcga gatcctcggc gttgcaaagg tccgtcgggt agtcccagcc gccgaacatg
     5761 cgcacgacga tccgcggacc gctggtgccg aaggtctcct ttcggcgcat cgcggaaaag
     5821 atcgcttcgc gcgcattctc ctccgcccag accgctgtca ggcctccggg gctgagatac
     5881 tcgttgtcgg gaaagccctg aggcggtgga aggaaacgat tcggctgccc cgcaccgccg
     5941 tggcccttga aggcattctc ggccacgaag ccaggcgcag agatgtgcgt gtcggtagcc
     6001 gcagtgatcc cgtattgaaa gggattggtg ccgagattgg cctcgagctt caaaccttct
     6061 ccgtaggcgt gacggagaaa gcccttcggg tcgggtgggg tgattcgctc ttggtccgtg
     6121 gtagcgaggt tggagaaggg gatcgactcg aaaccgcaga gctcgtccga gctcgctgcg
     6181 cccggtatgc actccgaagc tcccttgtgc tggtagatct cgatgacagg ctccatggcg
     6241 ttccgtaacg ccgcgtactc ggccgtgaac ggctcaccat tggccatctt gtcttggaag
     6301 tagatccctt cggagagatt gctgttgtgc gggatcgtga gcacgtcgca gccgttgccg
     6361 gcatcgaggc aaccctcgcg aaggtgtgcc cagaggccct cggcatacgg tgcatcgaag
     6421 taactgtagg gcagatccgt gaccttctcg ttcttgaaca tcacattgcg atgaaggttc
     6481 ttggccatgg ggccgccgct ccattcgtag ccaggaaagg aagtaaactc gcagctgtcg
     6541 ctccggtcat aggccccttc cgccttgctc acgatctcgg accagacatc catacccgcc
     6601 tgcaagcagc tctcgttcat gggcccgcag agtgcgggat aatgtgcctc gtcagggggg
     6661 aagatcgtga gcccgttgat ctcgacgaag atgaacgtga cctcggccgg cgtcgcgtca
     6721 aactgcatcg ctgcgcgata ctcgacgcat tgatcggcgt tgtagccggg cgacgttgga
     6781 tcattgcaaa ccttcagcgt cccgaggaac tcggcgtggt cgctcagcat cacgaagtcc
     6841 aagggtcggt cgatcgtggc gctccgagtc ggggagccgt ctgcggtgta gggctgaagc
     6901 gggatcgtcg caccgcgtgc aaaggcgtac gcgtcgtcct gcgacgttcg cgttccctgc
     6961 atgttggcat cgaacgaaag ctcggtgtga atgtgcgtgt cgccccagta gacctggcgc
     7021 agagggtcga aatccg
//
LOCUS       DASRZF010000120        10930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1804455, whole genome shotgun sequence.
ACCESSION   DASRZF010000120 DASRZF010000000
VERSION     DASRZF010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 10930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10930
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1804455"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VFG22_05555"
     CDS             complement(<1..290)
                     /locus_tag="VFG22_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415740.1"
                     /translation="MTHALHSTVSTQYWSRLQDGRLVCELCPRECKLGEGQRGLCFVR
                     AREGDEVVLTTYGRSSGFAVDPIEKKPLNHFLPGTPVFSFGTAGCNLTCKFC"
     gene            377..1210
                     /gene="amrB"
                     /locus_tag="VFG22_05560"
     CDS             377..1210
                     /gene="amrB"
                     /locus_tag="VFG22_05560"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HET6415741.1"
                     /translation="MGTARPAAVAGSFYPADRHALRLVVNEYLDDARQPSEVRDSWPK
                     ALIVPHAGYMYSGAVAASAYVQLEAARELIARVVIIGPSHHVAFDGLALPSSTAFETP
                     LGSVPIDETARREALHFPFVEVLDDAHHWEHSLEVQLPFLQEVLGSFSVLPIAVGRST
                     SGQVAELLSSVWDGDETLIVISSDLSHYHDYDTAKSMDLATARAIESLEPQRLASEQA
                     CGQIGIRGLLEAARLHGIRPRRLDLRSSGDTAGSRDEVVGYGAWELSPSGRRPSGRQS
                     P"
     gene            complement(1207..2844)
                     /locus_tag="VFG22_05565"
     CDS             complement(1207..2844)
                     /locus_tag="VFG22_05565"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HET6415742.1"
                     /translation="MRAASLVFGALVVLFGSAGAGCSSDESSAAPQITSAETSLGVAN
                     GFVTDEGVVAFLGLPFAEAPVGELRFRPPVPVESWDSPLDASEFGPACPQPVEPGQAL
                     YQNQSEDCLTLNVWTPSADGGRRPVMVWVHGGGWVYEGTEDPLYSGAKLAARGDVVVV
                     SMEYRLGAFGFSHFEDIEGSGNAGLLDQALAFEWVRDQIAAFGGDPDNVTLFGESAGG
                     MSATALMAMPRAKGLFHKAIAQSGAGGTAREAAYAKAVADKFLAAAGVSDVAELSEMT
                     TAELLAAQDLLIEDAFLEDLLFGTVVDGVVVPEPPIQAIVKGSAAGVPLLTGTTKDET
                     RLWIIYVELLANLPLDVVLSFVPSGARAIPDGKTVQDVVDLYAANRPGAEPGVVTHAA
                     GTDIFFRLPAIRLVEAQLAHQPNNTFLYRFDWPPPAPAYPEYDLGAPHASELAFMFGT
                     PEGWTEIYGTGGIPNELRDQMMDAWIAFAKTGDPNHPNMPSWPAYDTTDRPTLLFDTT
                     DAGPLTELVNDPAGDERAFWDGVPFDGVRPSSEPEDL"
     gene            2957..3682
                     /locus_tag="VFG22_05570"
     CDS             2957..3682
                     /locus_tag="VFG22_05570"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HET6415743.1"
                     /translation="MLRATSVALLVIGACTPSSGSTSPVPADTGNRVQERQQMVHAQI
                     EARGVTDARVLAAMRKVPRHRFMPESQRPYAYDDRPLPIGQAQTISQPYIVALMSELA
                     AVKPGDTVLEVGTGSGYQAAILAEMGAKVFSIEIVASLAQRAKAILDVLGYGGSVEVR
                     HGDGYAGWPERAPFDAIVVTAAPPKIPEPLKQQLEVGGRLVIPVGKDFQSLLRVTRTE
                     DGFREESVIPVRFVPMTGKAQEN"
     gene            complement(3723..4610)
                     /gene="cysD"
                     /locus_tag="VFG22_05575"
     CDS             complement(3723..4610)
                     /gene="cysD"
                     /locus_tag="VFG22_05575"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF003587.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HET6415744.1"
                     /translation="MDLAELEAESIFVIRETAAQFDKPALMFSGGKDSIVMAHLARKA
                     FHPARFPFPLLHVDTGHNFRETIEFRDRYASSVGAELIVASVQRSIDEGLVEEETGPY
                     ASRNALQIPTLLRAIEEHGFDACLGGGRRDEEKARAKERFFSHRDRFGQWDPKNQRPE
                     LWNLYSGIKRKGENFRVFPLSNWTELDVWRYIREERIELPSLYFAARRECLRRHGSIL
                     AISEFVQPRQGETVETKSIRFRTMGDAPITGAVESTASDLDGVIRELLSASQTERGNR
                     ADDQLSENAMEDRKKQGYF"
     gene            complement(4601..7159)
                     /locus_tag="VFG22_05580"
     CDS             complement(4601..7159)
                     /locus_tag="VFG22_05580"
                     /inference="COORDINATES: protein motif:HMM:NF021578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HET6415745.1"
                     /translation="MSQALVQEASSVENVDPSVEVSRPIPVQLDSPERSFRDLDLALS
                     KARTRVAPLWPLKHFVAVNPFLGLSSQRFEEACSTMREVAGARMLMPRAYYRGLIEEG
                     RILDEDLLEAMRAAQGVPGAPNDLQELLRAIETESTPEDASTVTVADLLDRLHGLRAS
                     PFVVEEIAKWCAAYWDEGQAAWRMPWRGLPLYAGWRAAACLDRTPDVMGFHGLRALVA
                     SLPEDPVHLIETSIEALGLSGPALDAYLHRALFSIRGWVAYARYLGWYQELDGEEDDA
                     VTQLLAIRLAWDYALFRLHGDELFSIRWKEAVRAMGEGASSGSRTYGSLALDVILQSA
                     FDLAFQRKLVEKLSVPPAASVLALARPPVQAAFCIDVRSEVFRRALEAVAPEAQTIGF
                     AGFFGFPIEYVPIGQHEGTAQCPVLLKPKFVVREQVAGADDDERTEILGLRLLRRRAS
                     KAWKSFKSSAVSSFVYVETAGLGYAAKLVGDASGITRTVTHPGAEGLDANVFSRIGPE
                     VETGTLAGRATGFTEDERIDAATTVLKAMSLRRGFGRLVMLAGHGSTTVNNPHASGYD
                     CGACGGNPGGANARVAAAILNDPQVRAGLADRGIVVPSDTWFLGCLHDTTTDEVTIFD
                     RHGIPSSHAQDLQRLEGWLAEASSIARAERAKLLGVSASADVDKAIVKRSRDWSQVRP
                     EWGLAGNAAFIAAPRARTRALDLEGRVFLHDYDWKQDEGWNVLELILTAPMVVASWIN
                     LQYFGSSVNQDAFGSGNKVLHNVVGTLGVLEGNGGDLRVGLPLQTVHDGERFVHEPLR
                     LSVFIEAPPEQIDAILRKHAKVRELVENGWLHLFALTDEGSSCLRRIAEGRWWT"
     gene            complement(7156..8772)
                     /locus_tag="VFG22_05585"
     CDS             complement(7156..8772)
                     /locus_tag="VFG22_05585"
                     /inference="COORDINATES: protein motif:HMM:NF006029.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HET6415746.1"
                     /translation="MPAQTLSIITLAPLALLAVGLVPARLANRRPQWMLGLARSATAL
                     AFGVALIAAALFFIAGNDPSARSLTLGIAGVGITVYYDALSAVMALLVSFVGIIVIGY
                     SRNYLAGDREQGRFIKWLCLTLAAVLTIIISGNLLQFTLAWVASSLCLHQLLLFYQDR
                     RAARLAARKKFIASRMGDLCLVAVMVLVYSLFGSLDFATVFGAADGLRDVESLPVSVH
                     VVAVLLVIGAMLKSAQFPFHGWLTEVMETPTPVSALLHAGIINAGGFLILRLSDVISL
                     SVPALEMLAVVGGFTALFGSVVMLTQTSIKVSLAWSTIAQMGFMMLQCGLGAFSAAAL
                     HIVAHSLYKAHAFLSSGSVIDLSRASWVARPHGKPHPARLVLALVGAVGITWAVSLAF
                     GASLTRAPGVFALGAILVMGVTHLVANAIDERPSVFVIGRGVGFAAAVAVAYFALQLG
                     AEHLMAGSLPAKQALRGPFDLTIVGLVVLLFGAVTVLQNTMPYRMSDPRWQAFYVHLF
                     NGFYVNAITNRLVQRWWPVLPASVNKGANP"
     gene            8872..9744
                     /locus_tag="VFG22_05590"
     CDS             8872..9744
                     /locus_tag="VFG22_05590"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET6415747.1"
                     /translation="MSDLNYHHLRYFWGIAHEGKLTQAAERFNVSQSALSTQVKKLED
                     QLGHALFEREGRGLKLTEAGRIALDYADTVFQAGEELLSTLEGRSGTERKVLRVGAIT
                     TLSRNFQIALLRPLLDRGDVELVVRSGTMRELLSQLEAHVVDLVLSNHPVARDAKSSW
                     HSHLLQVQPVSIVGPPAPDAQEFRFPEDLATMPILVPSLDSDVRVAFDRLLELAGIRP
                     TIVAEVDDMAMLRLFARDSDCLALVPPVVVRDELRDGILVERRKIPEITESFYAITQS
                     RRFPNPLVKELLQR"
     gene            9826..10779
                     /locus_tag="VFG22_05595"
     CDS             9826..10779
                     /locus_tag="VFG22_05595"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HET6415748.1"
                     /translation="MNKCLLLSSLTLALLMACDSGSSAPVGGSGGSTGTGGMGGTGGT
                     PRTCETDIDIGEVQPFGTTTPPRSEGITFDADGNLFVSAIDSMANDQLLSVTTEGTST
                     SVAEAESILGLASHANGIIAAAIDTGELLLIDPSTGESEPIATGLGAPNFVVTTPWDT
                     ILVSDDSIGEDSIDEVTWEGGVSNWVSGVPTPNGMVFSLDGRTLYVASTFQEPGLWRV
                     PVSAEGVAGTPEKWVTFPNATTPDGVAIDSEGNVYVALNVAGQIARVDPEGNATIIAE
                     NVNTVASLAFGQGDLDPCSIYATSLFSEQLWRVGIGVAGTE"
BASE COUNT         2120 a         3546 c         3396 g         1868 t
ORIGIN      
        1 tgacagaact tgcaggttag attgcagccc gccgtgccga aggagaacac gggcgttcct
       61 ggcaggaagt gattcagagg cttcttctcg attggatcca cggcgaagcc actcgaacgg
      121 ccatacgtgg tgagcacaac ctcgtctccc tcgcgcgcgc gcacgaagca gagccctcgc
      181 tgcccttccc ccagcttgca ctcacgtggg cagagctcgc aaacgagcct cccatcttgg
      241 agccggctcc aatactgcgt cgaaaccgtc gagtgaaggg catgggtcat gcattgcacg
      301 atgggcttcc gggagtcgcc tggcaaggcc cacgggcccg cattgacaag cctatcgtcg
      361 cgctcacatt cggtgcatgg gaactgcacg accggctgca gtggcgggtt cgttttatcc
      421 ggcggaccga cacgcattgc gcctggttgt gaacgagtac ctcgatgatg cgcgtcagcc
      481 ctcagaggtg cgcgactctt ggccgaaagc gctgatcgtg ccgcacgcgg gctacatgta
      541 ctccggtgca gttgcggcca gcgcctacgt gcagctcgaa gccgcgcggg agctcattgc
      601 gcgcgtcgta atcatcggtc cgagtcatca tgtggcgttc gacgggctcg cccttccgag
      661 cagcacggcc ttcgaaacgc cactcggatc ggtacccatc gatgaaaccg cgcgtcgcga
      721 ggcactgcac ttccccttcg tcgaggtgct cgacgatgcg catcactggg agcattcact
      781 ggaagtgcag ctcccgtttc ttcaagaagt gctgggcagc ttttccgtgc ttcctatcgc
      841 tgtcggccgc tcgacttcgg gacaagttgc cgagctcttg agctctgttt gggatggcga
      901 cgaaaccctg atcgtcatca gctcggatct cagccattac cacgactacg acacggccaa
      961 gagcatggat ctcgcaacgg cgcgagccat cgaatcgctc gagccccaac ggctcgcgtc
     1021 cgagcaggcc tgcggacaga tcgggatcag gggtctgctg gaagctgcgc gactgcacgg
     1081 aattcggccg cggcgcttgg acctccgaag ctcgggtgac accgcaggca gccgcgacga
     1141 ggtcgtcggc tacggcgcct gggagctcag cccgtctggc cgccgcccat ccggtcgtca
     1201 atctccctag aggtcttcag gctctgaact aggccttacc ccgtcgaacg gtaccccgtc
     1261 ccagaacgca cgctcgtcgc cggcggggtc gttgaccagc tcggtgagtg gacccgcatc
     1321 cgtcgtgtcg aagagaaggg tcggtcggtc cgtcgtatcg taagcaggcc agctcggcat
     1381 gtttggatga ttcgggtcgc cggtttttgc gaaggcgatc caggcgtcca tcatctggtc
     1441 gcgtaactcg ttcgggatcc cgccagtgcc atagatctcg gtccatccct ccggcgtgcc
     1501 aaacatgaac gccagctcgg acgcgtgcgg ggcgcccaag tcgtattccg ggtacgcggg
     1561 tgccggcggt ggccaatcga aacgatagag gaacgtgttg ttcggctggt gagcgagctg
     1621 cgcctcgact aagcgaatcg ccgggagcct gaagaagatg tccgtcccgg ccgcatgcgt
     1681 cacgactccg ggctccgccc cggggcgatt ggcggcataa aggtcgacga catcttggac
     1741 ggtttttccg tccggaatcg ctcgtgcacc cgacgggacg aaagacagga ccacgtcgag
     1801 aggcagattg gccaaaagct cgacatagat gatccaaagc cgcgtttcgt ctttcgtcgt
     1861 tccggtcaga aggggcacgc cggcagcgct gcccttgaca atcgcttgaa tggggggctc
     1921 gggcaccacg acgccatcga ccacggtgcc gaagagaaga tcctcgagaa acgcgtcctc
     1981 gatcaagagg tcctgcgcgg cgagcagctc agcggtcgtc atctcggaga gctcggccac
     2041 atcgctcacg cccgccgccg caagaaactt gtccgcaacg gctttcgcgt acgcagcctc
     2101 tcgcgctgtc cccccggcgc cgctctgtgc aatggccttg tggaagagtc ccttcgcacg
     2161 cggcatggcc atcagcgcgg tcgcgctcat cccgccggcg gattccccaa agagcgtcac
     2221 gttgtccggg tcgcccccga aggctgcgat ctggtcgcgc acccattcga aagcgagcgc
     2281 ctgatcgagc aagccggcat tgccgctgcc ctcgatgtct tcgaagtgac tgaagccgaa
     2341 ggcgcccagc cggtactcca tgctcacgac gacgacgtcg ccacgcgcag ccagcttcgc
     2401 gccactgtag agcgggtcct cggtgccctc gtagacccag ccgccgccat gcacccaaac
     2461 catcacgggg cgcctgccgc cgtccgccga aggcgtccaa acgttgagcg tcaggcagtc
     2521 ttcgctctgg ttctgataga gcgcctgccc gggctcgacg ggctgcggac aagcgggacc
     2581 aaactcgctg gcatcgaggg gactgtccca ggactcgacc ggaaccggcg gcctgaaccg
     2641 cagctctccg acgggcgcct ctgcaaacgg gagccccagg aacgcaacca cgccttcatc
     2701 ggtgacgaag ccattcgcga caccgaggct cgtctccgcg ctcgtgatct gaggcgccgc
     2761 gctactctcg tcgctcgaac agcctgcgcc tgccgagccg aaaagaacca ccaacgcccc
     2821 gaaaaccaaa cttgcagctc gcatcacgca cctttccaag cccggacgcc gctttgtagc
     2881 agccgccggt cgacccgtca ggcggaaggc caagacacgg ccgggacccc gtcgttctca
     2941 tgctaacgtc tttccaatgc tgagagctac aagcgtcgcg ctgctcgtca tcggcgcttg
     3001 cacaccgagc tcgggatcga cgagcccagt ccctgcggac actggcaacc gcgtccaaga
     3061 gcggcagcag atggtgcacg cgcaaatcga agcgcgcggc gttacggatg cacgggtgct
     3121 cgcggcaatg cgcaaagtgc cgcggcaccg tttcatgccc gagtcgcagc gcccctatgc
     3181 ctacgacgac cgtcccctgc cgatcggcca ggcacaaaca atcagccaac cctacatcgt
     3241 cgcgctgatg agcgagctcg ccgcggtcaa gcctggcgac acggtgctcg aggttggaac
     3301 gggctccgga taccaagccg caatcctcgc ggaaatgggt gcaaaggttt tctcgatcga
     3361 gattgtcgcg tctcttgcac aacgcgcgaa ggcgatcctc gatgtgctcg ggtacggcgg
     3421 gagcgtcgag gtgcgccacg gtgatggcta tgctggatgg ccagagcgcg cgccgttcga
     3481 cgccatcgtc gtgactgcgg cgccgccgaa gatcccggag cctttgaagc agcagctcga
     3541 ggtcggcggc cggctagtga tccccgtcgg caaggacttc caaagcctcc tgcgcgtgac
     3601 acggacggag gatggattcc gcgaagagtc ggtcatcccg gtgcgcttcg tgccgatgac
     3661 aggcaaggcg caagagaact agccggtctc gcgcccgcaa ccctgcgtcc gggatcttct
     3721 gctcagaagt acccctgctt ctttcgatct tccatcgcgt tctccgaaag ttggtcgtcc
     3781 gcacggtttc cgcgctcggt ctgcgacgcg ctcaagagct cacggatcac cccatcgaga
     3841 tcgctcgccg tagactcgac tgcgccagta atcggcgcgt ctcccatcgt gcgaaatcga
     3901 atgctcttgg tctcgacggt ttccccttgg cgtggctgca cgaactccga gatcgcgaga
     3961 atgctcccgt ggcggcgcag gcattcgcgc ctagcggcga agtacaggct cggcagctcg
     4021 atgcgctcct cccggatgta gcgccagaca tcgagctcgg tccaattgga gagtgggaac
     4081 acgcggaagt tctcaccctt ccgcttgatg ccactgtaga gattccagag ctcggggcgt
     4141 tgatttttcg ggtcccactg accaaagcga tcccgatgcg agaagaatcg ctctttcgcc
     4201 cgcgcctttt cttcgtcgcg acggccgccg cccaggcacg cgtcgaagcc atgttcctcg
     4261 atggcgcgaa gaagcgtcgg gatctgaagc gcgttgcgac tcgcgtaagg accggtctcc
     4321 tcctcgacca ggccctcgtc gatggacctt tgtacgctcg cgacgatgag ctctgcgccg
     4381 accgagctgg cgtaccggtc gcgaaattcg atggtctcgc gaaaattgtg accggtatcg
     4441 acgtggagaa gcggaaacgg aaagcgcgcc ggatggaacg ccttgcgggc gagatgagcc
     4501 atcacgatcg agtctttccc gccggaaaac atgagcgcgg gcttgtcgaa ctgggccgcg
     4561 gtttcgcgga tgacgaagat cgactccgct tcgagctcgg ctaggtccac caacgaccct
     4621 cagcgattcg ccggagacac gagcttccct catcggtgag cgcaaagaga tgcagccaac
     4681 cgttctcgac cagctcgcgg accttcgcgt gctttcgcag aatcgcgtcg atctgctcgg
     4741 gcggagcctc gatgaagaca ctcagtcgga ggggctcgtg gacgaagcgc tccccgtcgt
     4801 ggacggtttg caagggcagt cctactcgca aatctccacc attgccctcg aggacaccca
     4861 gcgtcccaac cacgttgtga agaaccttgt tgcccgagcc gaaggcatcc tgattcaccg
     4921 acgaaccgaa gtactgcagg ttgatccagc tcgcgaccac catgggcgca gtcaatatca
     4981 gctcgagaac gttccagccc tcgtcctgtt tccagtcgta gtcgtgaagg aacacccggc
     5041 cttcgaggtc gagcgctcgc gtgcgggcgc gtggcgcggc aatgaaagcc gcgttgcctg
     5101 ctaggcccca ctcagggcgc acttgcgacc agtcgcgact ccgcttcacg atggctttgt
     5161 cgacatcggc cgatgcggaa accccgagca gcttcgcccg ttccgcgcgc gcaatcgacg
     5221 acgcctccgc gagccagccc tcgagacgtt gaagatcttg ggcgtgcgac gagggaatgc
     5281 catgtcggtc gaagatggtg acctcgtcgg tcgtggtgtc gtggaggcat ccgaggaacc
     5341 aggtgtcgct cggaacgacg atgcctcggt cggcgaggcc agcgcgcact tgtggatcat
     5401 tgaggatggc ggcggccacg cgggcgttcg cccctccggg gttgccgccg caagcgccac
     5461 agtcgtagcc cgatgcatgg gggttgttga ccgtggtgct cccatgcccg gcgagcatca
     5521 cgagtcgccc gaaaccacgc ctcagcgaca tcgccttgag cacggtggtc gcagcgtcga
     5581 ttcgctcgtc ctcagtgaag ccggtcgcgc gccccgcgag ggtacccgtt tcgacctctg
     5641 gcccgatccg cgaaaacaca ttggcatcga gcccttcggc gcctgggtgc gtcaccgttc
     5701 gggtgattcc gctcgcgtcc ccgacgagct ttgcggcgta cccgagcccc gcggtctcga
     5761 cgtagacgaa cgaggagacc gccgagctct tgaacgactt ccatgctttg ctcgcacgac
     5821 gacgaagcaa gcgcagaccc agaatctcgg tccgctcgtc gtcgtcggct ccggccactt
     5881 gctcgcggac cacgaacttc ggtttcagaa ggacggggca ctgtgccgtg ccctcgtgct
     5941 gtccgatggg cacgtactcg atggggaatc caaagaaacc ggcgaatccg atcgtctgtg
     6001 cctcaggcgc gaccgcctcg agggcacgcc ggaagacctc ggaccgcacg tcgatacaaa
     6061 acgcggcctg taccggcggg cgcgccaagg cgaggacgga agccgccggc ggtaccgaaa
     6121 gcttctcgac gagcttgcgc tgaaacgcca gatcgaatgc ggactgcaga atgacgtcaa
     6181 gcgccagact cccataggtc cgggaacccg acgacgcgcc ctcgcccatc gcccgcacgg
     6241 cttccttcca gcggatgcta aacagctcgt cgccgtggag tcggaagagc gcgtagtccc
     6301 atgcgagtcg aatggcgaga agctgagtca ccgcatcgtc ctcttcgcca tcgagctctt
     6361 gataccagcc gagatacctc gcgtaggcga cccagccgcg aatgctgaag agcgcacgat
     6421 gcaggtacgc atcgagcgca ggtccggata gccccaaggc ttcgatgctg gtctcgatga
     6481 ggtgaaccgg atcctccgga agggaagcga ccagcgcgcg aagaccatgg aaacccatca
     6541 cgtcaggggt gcggtcgagg caggctgcgg cccgccagcc cgcgtacagc ggtaagcctc
     6601 gccatggcat gcgccaagcc gcctgccctt cgtcccagta cgctgcacac cacttggcga
     6661 tttcttccac cacgaaaggc gaggccctaa gcccgtgtaa tcgatccagc agatcggcga
     6721 cggtgaccgt gcttgcgtcc tcaggggtcg actcggtttc aatcgcgcgg aggagctctt
     6781 gaaggtcgtt tggagcaccc ggaaccccct gagccgctcg catggcttcg aggaggtcct
     6841 cgtcgaggat tcgtccttct tcgatgagtc ctcggtagta ggcgcggggc atgagcattc
     6901 gggcgcccgc aacctcccgc atcgtcgagc aggcctcttc aaaccgctga ctcgagaggc
     6961 cgaggaaggg attgaccgcg acgaaatgct tgagcggcca aagtggtgcg actcgagtgc
     7021 gcgccttgct gagcgcgaga tcgagatccc gaaagctacg ctccggtgaa tcgagttgaa
     7081 ccggaatggg tcgggaaact tcgacggacg gatcgacgtt ctcgaccgag gacgcctctt
     7141 ggacgagtgc ttggctcatg ggttcgctcc cttgttgacg gaggccggaa gaaccggcca
     7201 ccagcgctga acgagacggt tggtgatggc attcacgtag aatccattga agaggtgcac
     7261 gtagaacgcc tgccagcgag gatcggacat gcggtacggc atcgtgtttt gcagaacggt
     7321 cacggcgccg aagagcagaa ccaccaatcc gacgatggtc aggtcgaatg ggccgcggag
     7381 cgcctgcttg gcaggtagac ttccggccat gagatgctcg gcgccgagct gaagtgcgaa
     7441 gtacgcgacc gccacggcag ctgcgaatcc gacgcccctt ccgataacga agacgctagg
     7501 acgctcatcg atggcgttgg cgactagatg cgtaacaccc atcaccagaa ttgcgccgag
     7561 cgcaaagacc cctggcgcac gcgtgagtga agcgccgaac gcgaggctca cggcccaggt
     7621 gattccgacc gcacccacga gcgccagcac caggcgggcc gggtgcggct taccgtgagg
     7681 ccgggccacc caagaggcgc gggagaggtc gatgacgctc ccggacgaca ggaatgcatg
     7741 cgccttgtag agcgagtgcg cgacgatgtg aagcgcggcc gccgaaaagg cgccgagccc
     7801 gcactggagc atcatgaagc ccatctgcgc gatggtggac caggcgagtg aaaccttgat
     7861 gctcgtctgt gtgagcatga cgaccgaacc aaagagcgcc gtgaatccgc cgaccaccgc
     7921 gagcatctcg agggccggca ccgagagcga gatcacatcg ctgagccgca aaatgagaaa
     7981 accacctgca ttgatgatgc ccgcgtgaag cagcgccgaa acgggagtcg gagtctccat
     8041 gacttcggtc agccagccgt ggaacggaaa ctgtgccgac ttgagcatcg ctccgatgac
     8101 gaggagcacg gcgacgacgt gtaccgacac gggcagtgac tcgacgtcgc ggagcccgtc
     8161 ggccgcgccg aagaccgtcg cgaaatcgag gctgccaaac agcgagtaga cgaggaccat
     8221 caccgcgacg aggcagaggt cccccatccg gctcgcgatg aacttcttgc gggcggcgag
     8281 ccgggcggcg cggcggtcct ggtagaagag cagaagctga tgcaggcaga ggctgctcgc
     8341 cacccaggcc aaggtgaact gcagcagatt gcccgagatg atgatcgtca acacagccgc
     8401 aagcgtgagg cagagccact tgatgaaacg gccctgctcc cggtccccgg cgaggtagtt
     8461 tcggctgtac ccgatgacga tgatcccgac gaaggagacg agcagcgcca tcacggccga
     8521 aagcgcgtcg tagtaaaccg tgattccgac acccgcgatg ccaagggtca ggctccgggc
     8581 cgatggatca ttccccgcaa tgaagaagag cgcggccgcg attagggcca ccccaaaggc
     8641 tagagccgtc gccgagcggg ccaatcccag catccactga gggcggcgat tggccagtcg
     8701 ggccggtacc agccctacgg cgagtagggc caatggggcg agggtgatga tggataaggt
     8761 ctgggccggc atgaatcctc cgctaagaga cggggattac ttagtgacgg acttccattc
     8821 tgtaaaatac aaagattgaa actcatcgtt ctttattata gaacattgcg catgtcagac
     8881 ctcaactatc accatctccg atacttctgg ggcatcgccc atgagggaaa gctcacgcag
     8941 gccgccgagc gattcaacgt ctcccagtcc gccctctcga ctcaggtgaa gaagctcgaa
     9001 gaccaactcg ggcacgcgct gttcgagcgt gagggacgcg gcctcaagct gaccgaggcc
     9061 ggccgcattg cgctcgacta cgccgacacc gtctttcaag ccggcgagga gctgctgagc
     9121 accctcgaag gccgcagcgg aaccgagcgc aaggttctgc gcgtgggggc gatcacgacc
     9181 ttgtcgcgaa actttcagat cgcactgctc cgtccgctcc tcgatcgagg cgacgtagag
     9241 ctggtcgttc gttccggtac gatgcgcgag ctgctctctc agctggaagc gcacgtggtc
     9301 gacttggtcc tctcgaacca tcctgtggcg cgcgacgcca aatcgtcgtg gcacagccac
     9361 ttgctgcaag tgcaacccgt cagcatcgta ggtccgcccg cgcccgacgc gcaggagttc
     9421 cgtttccccg aggacctggc cacgatgccg atcctcgtgc cgagcctcga cagtgatgtg
     9481 cgggtggcct tcgaccggct gctagagctc gctggaatcc gacccacgat cgtcgccgag
     9541 gtcgacgaca tggcgatgct tcgcctcttt gcgcgcgaca gcgattgcct ggccctcgtc
     9601 ccaccggtcg tcgtgagaga cgagctccgc gacggaatcc tcgtcgaacg gcgcaagatc
     9661 cccgagatca ccgaaagctt ctacgccatc acccagagcc gccgctttcc gaaccccttg
     9721 gtcaaagagc tgcttcagcg ctgaccctcg cagcacgcgc aaagctgcgt gacagcgcac
     9781 ccacggctgg ttagtctccg cttcccaatc gaacgaggtg gtccgatgaa caagtgtttg
     9841 cttctttctt cccttacgct ggcgctgctc atggcctgtg actccggctc gagcgcaccc
     9901 gtcggaggct ccgggggcag taccggaacc gggggaatgg gcggcaccgg tgggacgcct
     9961 cggacctgcg aaacggacat cgacatcggc gaggtgcagc ccttcggcac gacgacccca
    10021 ccgaggagcg agggcatcac cttcgacgcc gatggaaacc tgttcgtctc cgcgatcgac
    10081 agcatggcga acgaccagct cctgtccgtc acaacggagg gcacatccac cagcgtcgcc
    10141 gaagcggaat cgatcctcgg tctcgcgagc cacgcgaacg gcatcatcgc cgctgccatc
    10201 gacaccggag agcttctgct catcgatcct tccaccggcg aatcggaacc gatcgcgacc
    10261 ggactcggcg cccccaactt cgtcgtcacc accccatggg acacgatcct cgtttccgac
    10321 gacagtatcg gcgaggattc gatcgacgaa gtgacctggg agggaggcgt ttcgaactgg
    10381 gtttcgggag tgccgacccc gaacggaatg gtgttctctc tcgatggacg cactctgtac
    10441 gtcgcatcga ccttccagga gcctggcctt tggagggtgc cggtttccgc tgagggagtg
    10501 gcaggaacgc ccgagaaatg ggtcaccttc cccaacgcca ccacgccgga cggcgtcgcc
    10561 atcgacagcg agggcaacgt ctacgtcgct ctcaacgtgg ccggccagat cgctcgagtc
    10621 gatccggagg gcaacgcgac catcatcgcg gagaacgtca acaccgtcgc gagcctggcc
    10681 ttcggccaag gcgacctcga cccctgctcg atttacgcaa ccagtctctt cagcgagcaa
    10741 ctctggcgcg tcggcatcgg cgtcgccggc actgaatagc caacgaccaa gaaggttctg
    10801 tgagcgatcc aagcgctctg ggtatcagac gcgaaccctc gaccacgacc atctggtcac
    10861 ggatgcggaa gacccaggtg taggtttttc ttggacatct ccgacgaatt tccacgatac
    10921 tcgccgccat
//
LOCUS       DASRZF010000121        11279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2149629, whole genome shotgun sequence.
ACCESSION   DASRZF010000121 DASRZF010000000
VERSION     DASRZF010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 11279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11279
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2149629"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="VFG22_05600"
     CDS             complement(<1..1115)
                     /locus_tag="VFG22_05600"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6415749.1"
                     /translation="MATENALELVRLGHFTDPEHAPYKVAHRLPIAKLRRYGEEGHRP
                     TVNAPLLLIPPLMVTAEIYDVAPDISGVTALTRLGLDVWVIDFGSPEQEEGGMKRTLD
                     DHIKAVSDSIDWVREATGHDVHLVGYSQGGMFAYQTAAYRESKGIASLVTFGSPVDIH
                     RNLPMVEDSIAGRIFELAQGALDTPLDKLEGLPGILTSTGFKLLAVHKEVGQLVDFVK
                     KLHDRQALEKREARRRFLGGEGFVAWPGPALKKFIDEFVVHNRMLSGGFVVDGKTVTL
                     ADITCPVMFFVGERDTIANESAIRAIRGAAPNAELFEVSMRAGHFGLVVGNQAMSFTW
                     PTVASWVRWREGLGPRPSVLDAPPVLQEPEEADFDEV"
     gene            1227..3113
                     /locus_tag="VFG22_05605"
     CDS             1227..3113
                     /locus_tag="VFG22_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415750.1"
                     /translation="MSQTPGAMTVLASITMPAFSDDDLATLRRVEAVLARHPYMRADL
                     GARGAISRELEAVLSTRLALLHTLGAPDACTEVTSLQDKLRAWELELAEAAAEEDGSE
                     RTRLGYETTLLLHPAPALPQESQLTRATIATVTRAWECLREAQSLRSVIANKARQSRD
                     FIRHGAMLPLYWMRRRRIRKLLPDVLLSHPGLRETLFAIEQIGPLVDNFAFKGKAGVP
                     WGTSVALADLTFLYMQLADEFLDELAAAAGGHDEAGRLVRSVYRDDTSKRPLWDLSLG
                     HLLAIGVDPEAHATKFGLSLSALFDTLERLAAAIDALLADASGAIVHATHLFLHHCFQ
                     TYLDEAALCERALGRRVDRMRLQDTGWHFYRKNNMVMMLWLDLRARVLGLEPVSHAEE
                     IRRWGYLLAAFQIFDDLKDIALDLGKQPSYPLQIAAHEFPSELAWLERKFGAQRLPMT
                     RDQVPEVNLRASGTVQQCMRWSRLIALAHFDNTLLYAWDQRWSKSWTRRRRSFNPAGE
                     VMTKRRDHVIDRLVRALLSLRRADAPPTVDDSQLAFALDASAYEGSWQIYLALLPNVR
                     AVYRFATLRMWMSAEEKARAARQLLRRYPRARANALVGLANADVDHQVTGDRLEAFSK
                     LIEI"
     gene            complement(3030..3548)
                     /locus_tag="VFG22_05610"
     CDS             complement(3030..3548)
                     /locus_tag="VFG22_05610"
                     /inference="COORDINATES: protein motif:HMM:NF025116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HET6415751.1"
                     /translation="MESLVVTVIGKDRPGLVESVSAAVEAHGGSWVESRMSRLAGQFA
                     GILRVSVPSAEADALSDALEALRAGGLRLVIERGVEDAEEDGHVILLELVGSDRPGIV
                     HKISEALAARGVNVDELNTECEGAPWSGGTLFKAMAKLRAPRTLDLDQLRESLEAIAG
                     DLMVDISIGEAD"
     gene            3688..4830
                     /locus_tag="VFG22_05615"
     CDS             3688..4830
                     /locus_tag="VFG22_05615"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET6415752.1"
                     /translation="MFAKRTKEVEPFLAMEVMERGMAMARAGTSIVQLGVGEPNFDAP
                     PAAVQAAVDSLVSGETHYTDSRGVFALREAIAKDCEQRRGVVVSPDRVVVTSGTSPAI
                     LLCLNLLVDEGDEVIMATPHYPCYPNMIAVCGGTPVLVPTGPEDGFIMDVAKVRAAIT
                     PRTRAIFLASPANPTGAVQPREVVEELSGLGIPILSDEIYDGLTYDGAEVYSPLRFTE
                     NCYVLDGFSKRYAMTGFRLGYLIAPEIAMRPLQSLMQSFFISTSQFVQQAGVAALAEG
                     APHVEYMRQQYAIRRRVLIDGLREMGLSIPIDPPGAFYVLVDMRHFGRDSLTLSFDIL
                     EKAHVALGPGRDFGEAAEGFVRFSFATSLEQIEEGLRRLGRALPEL"
     gene            complement(4834..6918)
                     /gene="ligA"
                     /locus_tag="VFG22_05620"
     CDS             complement(4834..6918)
                     /gene="ligA"
                     /locus_tag="VFG22_05620"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HET6415753.1"
                     /translation="MSDRARAAKELEQLSQQIRYHEAAYRAGAPEIPDAAFDELVERY
                     QELADLLGIDAAERIDEQPGEDHTAGFETVEHRVPMLSLEKLSPNRRDSAGVQIPISE
                     QLSAWFRRRLKELEVDALPVIVEPKVDGISVSLLYEAGRLRQAVTRGDGHRGDVITQQ
                     VAQLGTVPTKFRESGKGSLEIRGELYWPLSEFTRFNATLEKRLINPRNGCAGMMKRKD
                     PTGIEETGIRAFFYQVAWSDGVELPSTQYETLQWLADRGAEVYSSEVFQTTSDEAALQ
                     YCDDYQARRESLDYDIDGMVIKIDELGFYTRLGATGHHPHWGIAYKFPPERKPTKLLE
                     IEVSVGKSGKLTPVAILEPVFVSGTTVSRASLHNFVELERKDVRVGDTVLVEKAGEII
                     PQVVEVDASKRKRGAKRYVPPKSCPTCGTEAVSEEIFIYCPNPACPDQVRERLRHFAS
                     RGAMDIDGMGEVLVDQVVDKLGVRSPDDLFRLEVDAIAGLDRMGQKSAERVKAGLEAA
                     KDRGLARVLNGLAIRHVGETMSEDLSAYFKTADALLEFAARYVAEDEDAIETVAPAKS
                     AERGAIEGLARKSADSIFHELNSPAVRKVFAGLADVGVNLTAKTADVRPVADVAGKTF
                     VLTGALPTLTRTEATKRIKAAGGKASGSVSKKTDYVVAGDDPGSKYEKALALGVEILD
                     EAGLLALLGEEG"
     gene            7026..9629
                     /locus_tag="VFG22_05625"
     CDS             7026..9629
                     /locus_tag="VFG22_05625"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6415754.1"
                     /translation="MDDEGQRPGGLGDGSMARYERERDEQTRLRSLGALSHMLPVLSF
                     IYAAAAALFLLAPPFTPNHPHVFICAISSALVLCVWAGMRVDDPRFDTPISVATISVA
                     AGMGLTMFAVSGAISNTTTLAIAIVAAGALLFRFSMYAVMVAVVFMGWIPLAYGYENG
                     AVSLGIFHLWAATVVGGLVLWSRRQLMFGLHDEAHEAEAMRALATEQARSLARARDAA
                     LASAQVKGQFLANVSHEVRTPLNGILGLLQLIDPSALPKPQDEYLLEVNKSSGSLLAI
                     VNDLLDLSKIEAGEMRLESVAFDVIGMTEEIAMNYASAAQAKSIELITQIAPDVPTEV
                     CGDPLRLRQVMSNLLNNAMKFTKDGEVVIGVRVRDRGPWHVNLEFRISDTGVGVSKER
                     AESIFRPFSQADSSTTREYGGTGLGLPICRQLVELMGGDLQLESTLGQGSTFFFEARF
                     ELEERLSAELKAVTAALSGMRVLVLEGNNRARETLCAQLTAWKMEALATTTCDGALGA
                     LRGTPAPNVALIDLRSLGADWRNRVSELSKVATERGTSVVAVCSHKHEQSELLESGVR
                     VHVEKPIRRTKIIAALLETLKRASVPVRSIEEPRNPSLPPPPPKDLVSNGMKILVAED
                     NSINQKVVRAHLRALGYDVDTVDDGAAALEALKDGHPYAAVIMDGQMPNMDGYEATRA
                     QRARELESGQGRVPIIALTAHAMTGDRRTAFAAGMDDYLSKPFTQKQLQKALARWAAR
                     PSSSLSEFPPDALDSSITSQLLELEEEEPGFLCEVIHSFFQTAEDCISRMSKAIDAGD
                     LKSLRAAAHMVRGSSQQLGASRFGATCFKLEQLECMDEASVLLGALKDDLEGARGSLT
                     ELADRALDAAS"
     gene            complement(9640..10446)
                     /locus_tag="VFG22_05630"
     CDS             complement(9640..10446)
                     /locus_tag="VFG22_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415755.1"
                     /translation="MTYLVLQMVGSLVAAAVLGAALLWLGQVFWGRLVAPVRAKTLEY
                     ELEETRADMITLNERIRALHKELSASSAKIEALERAKRQLTATLDARNRTMQEVSTRL
                     SVIQPEAQPGSRESAEDLRRELRTAVQERDDARATLRQAETRGERLRAELEVLHGIHE
                     RMLDDIERLKARIREAELEVRSTGESRPPAWLMVQPFGARDDLQRLDGVDPSLERTLN
                     RLGIYHFHQIARFEPSDVEWLVEHVDGVPEQTIRDSWIVDASRLAGVQRN"
     gene            complement(10443..10760)
                     /locus_tag="VFG22_05635"
     CDS             complement(10443..10760)
                     /locus_tag="VFG22_05635"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HET6415756.1"
                     /translation="MWKKAVFGTVSAGFLGYVVWVSMLSPMRHEIEWDVATRVSGVLQ
                     RTGFSDIIPIVDGRDVELRGEVASASEALRAERIVSMVRGVRVVSAQLQVADDESLGG
                     GET"
     gene            complement(10902..>11279)
                     /locus_tag="VFG22_05640"
     CDS             complement(10902..>11279)
                     /locus_tag="VFG22_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415757.1"
                     /translation="FPSFRRVTGSFTGVEPLGHPRKPSALSAMVDRGRKELSEMIAEA
                     RGPLLKAAGAPEDDRPPLPHMTLARIQRRANSAERRAALAWAKAIDLDAVTFNAPSVA
                     LYTWSDDRQERLFRIVEQMSFER"
BASE COUNT         2044 a         3734 c         3424 g         2077 t
ORIGIN      
        1 tcgacttcgt cgaagtccgc ttcctctggc tcttgcagta ccggcggcgc gtcgagcacc
       61 gagggtcgag gtccgaggcc ctctctccag cgaacccaac tcgccacggt aggccaagtg
      121 aacgacatgg cctgattacc gaccaccagg ccaaagtgcc ctgcccgcat cgacacctcg
      181 aacagctccg cgttgggagc ggcgccgcga atcgcgcgaa tcgcggactc attggcgatc
      241 gtgtcacgct cgccgacgaa gaacatgacc gggcacgtga tgtccgcgag cgtgacggtc
      301 ttcccgtcta cgacgaaacc gccggaaagc atccgattgt gcacgacgaa ctcgtcgatg
      361 aatttcttga gggcgggccc aggccaggcc acgaagcctt cgcctcctag gaaccgccgg
      421 cgcgcttcgc gcttctcgag cgcctgtcgg tcgtgcagct tcttcacgaa atccacgagc
      481 tggcccacct ctttgtgaac agccagcaac ttgaatccgg tgctcgtgag gattcccgga
      541 aggccttcga gcttgtcgag cggcgtgtcc aacgcgccct gagcgagctc gaagatgcgg
      601 ccggcgatgc tgtcttcgac catcgggagg tttcgatgaa tgtcgacggg gctcccgaaa
      661 gtcaccaagg acgcgatccc tttcgactca cggtatgccg ccgtttggta ggcgaacatt
      721 cctccctgcg agtatccgac gaggtgcacg tcgtgaccgg tcgcctctcg cacccagtcg
      781 atcgagtcac tgaccgcctt gatgtggtca tcgagggtgc gtttcatgcc gccctcttcc
      841 tgctccggcg agccgaagtc gatgacccat acgtcgagcc cgagacgagt gagcgcagtg
      901 accccgctga tgtcgggagc cacgtcgtag atctcggcgg tgaccatcaa gggtggaatc
      961 agaagcaggg gagcgttgac ggtcggccga tggccttcct ctccgtagcg ccggagcttg
     1021 gcaatgggca gccggtgggc aactttgtac ggtgcgtgct ccgggtcggt gaaatggccc
     1081 aggcgaacga gctcgagcgc attctcggtc gccattgcga ttcgccgaaa aggcaggcgc
     1141 cagcttgatt ttcggcctct gaccgtcatc gtccgaatcg tgaccctagc accaagcaag
     1201 agggaattgg agcctgctga gctgccatgt cacaaacccc tggcgcaatg acggtactcg
     1261 cttccatcac gatgcccgcg ttttcggacg acgacttggc cacgctgcga agggtggaag
     1321 cggtgctcgc gcggcacccg tacatgcggg cggatctcgg cgcacggggt gcgatttcga
     1381 gagagctcga agccgtgctc agcacgaggt tggcccttct gcacaccctc ggtgcgccgg
     1441 acgcttgcac cgaggtcacg tcgctccagg acaaattgcg cgcctgggag ttggagcttg
     1501 cggaagccgc ggccgaagaa gatggctcgg aacggacccg gctcggctac gagacgacgc
     1561 tcctcctcca ccccgcgccc gcgctcccac aagagtcgca gctgaccagg gcaacgatcg
     1621 caactgtcac gcgcgcctgg gagtgcttgc gcgaggctca aagcctgcgc tcggtcatcg
     1681 cgaataaggc tcggcagagc cgcgacttca ttcggcacgg cgcgatgctt ccgctttact
     1741 ggatgcgtcg acgacggatt cgcaagctgc ttcctgacgt cttgctctcg cacccagggc
     1801 ttcgagaaac gctcttcgcg atcgaacaga tcggaccgct cgtcgacaac ttcgccttca
     1861 aaggaaaggc cggcgttccc tggggcacat cggtcgctct cgctgatctg accttcttgt
     1921 acatgcagct cgccgacgag ttcctcgatg agctcgcggc agcggctggc gggcacgacg
     1981 aagcagggcg gctcgtgcgc tcggtatacc gcgatgacac cagcaagcgc cctctctggg
     2041 atctctcgct cggacatctt ctcgcgattg gggtagaccc cgaggcgcac gcgacgaagt
     2101 tcggcctcag tctctccgcg ttgttcgaca cgctcgaacg gctggcggcc gctatcgacg
     2161 cgcttctcgc cgacgcgagc ggcgcgatcg tgcacgcgac ccacctcttt ctgcatcatt
     2221 gcttccaaac ctatttggac gaagcggcgc tctgcgaacg cgcgctgggc cgtcgcgtcg
     2281 accgcatgcg gcttcaagac acgggctggc acttctaccg gaagaacaac atggtgatga
     2341 tgctttggct cgacctgcgc gctcgcgtcc tcggtctcga accggtgagc cacgcggagg
     2401 agattcggcg ctggggctac ctgctggcag cgtttcagat cttcgacgac ttgaaagaca
     2461 tcgcgctcga cctcggcaaa cagccgagct acccgctgca aatcgcagcg cacgagtttc
     2521 cctcggagct cgcctggctc gaacggaagt tcggcgcgca aaggctcccg atgacgcgcg
     2581 accaagtgcc ggaggtcaac ctccgtgcga gcggaacggt tcagcaatgc atgcgctgga
     2641 gtcggctgat cgcgctcgcg cacttcgaca acacgctgct ctacgcctgg gatcagcgct
     2701 ggagcaagag ctggacccgg cgccgacgct ccttcaaccc ggctggcgaa gtgatgacca
     2761 aacggcgcga ccacgtaatc gatcgattgg tacgcgcact gctgagcctc cgcagagccg
     2821 atgcgcctcc aacggtcgac gactcgcagc tcgcgtttgc cctggacgca agcgcttacg
     2881 aggggtcctg gcagatctac ctggcgctct tgcccaacgt tagggccgtt tatcgcttcg
     2941 caacgctgcg gatgtggatg agcgccgagg agaaggcgcg ggctgcgaga caactcctgc
     3001 gccgctaccc gcgcgcgcga gccaatgccc tagtcggcct cgccaatgct gatgtcgacc
     3061 atcaggtcac cggcgatcgc ctcgaggctt tctcgaagtt gatcgagatc taacgttctc
     3121 ggcgcgcgaa gcttggccat cgccttgaac agggttcccc ccgaccaggg cgcgccctcg
     3181 cactcggtgt tgagctcgtc cacgttgacg ccgcgcgccg cgagcgcctc cgagatcttg
     3241 tgcacgatgc ccggccgatc gctgccgacg agctcgagca agatcacgtg cccgtcctcc
     3301 tcggcgtcct cgacgccgcg ctcgatcacc aagcgaaggc cccctgcgcg caaggcttcg
     3361 agtgcgtcgc tcaatgcatc ggcctcggcg gagggcacgc tcacacggag aatcccggca
     3421 aactgcccgg ccaagcgcga catccggctc tccacccagc ttccgccgtg cgcctcgacg
     3481 gcggcggaca cggactcgac caagccgggt cgatccttgc caatcacggt tactacgagc
     3541 gactccatcg gactcgctcc atacccgtaa tcgcccgaga ttccaaggtt ttataggcga
     3601 gaaccgaggc acaaatgccc gcggggtaga atcgctgccg aagaaccggc tagatctcaa
     3661 gcagttaggg acctagagcc tgttccgatg ttcgcgaagc gtacgaaaga ggtggagccg
     3721 ttcctcgcca tggaggtgat ggaacgcggc atggcgatgg cacgcgccgg aacgtcgatc
     3781 gttcagctgg gtgtggggga gccgaatttc gatgcgccgc ccgccgccgt gcaagcggcc
     3841 gtcgactccc tggtctcggg agagacgcac tacaccgaca gccggggcgt gttcgcgctc
     3901 cgcgaagcga tcgcaaagga ctgcgagcag cgccgcggcg tggtcgtcag ccccgaccgt
     3961 gtggtggtca ccagcgggac ctctcccgca atcttgctct gcttgaacct gctcgtggat
     4021 gagggcgacg aggtcatcat ggccacgccg cactaccctt gctatccgaa catgatcgcc
     4081 gtctgcggcg gtacgcccgt gctcgtgccg accggtcccg aagacggctt catcatggac
     4141 gtcgcaaaag tgcgggcagc gatcacgcca aggacgcgcg caatcttcct cgcatcgccc
     4201 gccaatccga cgggcgctgt acaaccgagg gaagtcgtcg aggagctcag cggtctcggc
     4261 attccgatcc tctcggacga aatctacgac ggcctgacgt acgatggcgc ggaggtctac
     4321 tctccgctgc gcttcaccga aaactgctac gtgctggacg gtttctcgaa acgttacgcg
     4381 atgaccgggt tccgcctcgg atacctgatc gcgcctgaaa tcgcgatgcg gccgctgcag
     4441 agtctcatgc agagcttctt catctcgacg tctcagttcg tacagcaggc tggagtcgcc
     4501 gcgctggccg agggcgcccc gcacgtcgaa tacatgcgtc agcagtacgc gatccgccga
     4561 agagtcctca tagacggcct tcgcgagatg gggctttcga tcccgatcga cccgccaggc
     4621 gcgttctatg tccttgtcga catgcgtcac ttcgggcgcg attcgctcac gctctcgttc
     4681 gacattctcg agaaggcaca cgtcgctctc ggaccgggac gcgacttcgg agaagccgca
     4741 gagggcttcg ttcgcttcag cttcgcgact tcgctcgaac agatcgaaga aggcctacgc
     4801 cgcctcgggc gcgcgcttcc cgagctctga agatcagccc tcttcgccga gcaaggccag
     4861 caagcccgct tcgtcgagga tctccacccc gagagccagc gccttttcgt acttgctccc
     4921 tgggtcgtcc ccagcgacga catagtcggt cttcttcgaa acggagccgg acgccttccc
     4981 acccgccgcc ttgatgcgct tggtcgcttc ggtgcgcgtg agcgtgggca atgcgcccgt
     5041 caagacgaac gtcttgcccg ccacatcggc caccggacgg acgtcggccg ttttcgccgt
     5101 caggttcacg cccacatcgg cgaggcccgc gaacaccttg cgcactgcgg gtgaattgag
     5161 ctcgtgaaag atgctatcgg ccgacttgcg agccaaccct tcgatcgctc cacgctccgc
     5221 agatttcgcc ggcgccaccg tctcgatcgc gtcttcgtcc tcggctacgt atcgcgctgc
     5281 gaactcgagc agtgcatcgg cggtcttgaa gtaggcggac aagtcctccg acatggtctc
     5341 gcccacgtgg cgaatcgcaa ggccgttcaa gacgcgcgcc aggccccgat ctttggccgc
     5401 ttcgagccca gccttgacgc gctcggcgct cttctgcccc atccgatcta gtcctgcgat
     5461 cgcatctacc tcgaggcgaa acaagtcatc cggcgagcgc acgccgagct tgtcgacgac
     5521 ctggtcgacg agcacctcgc ccatgccgtc gatgtccatc gcaccgcggc tcgcgaagtg
     5581 gcgcagccgc tcgcgcacct ggtcggggca cgcgggattg gggcagtaga tgaagatctc
     5641 ttcgctcacg gcctcggttc cgcaggtcgg gcacgatttc ggaggaacgt agcgctttgc
     5701 accgcgtttt cgcttgctcg catcgacctc gacgacctgc gggatgatct cccccgcctt
     5761 ctcgaccagg accgtgtcgc caacgcggac gtccttgcgc tctagctcga cgaagttgtg
     5821 aagcgacgcg cggctcaccg tcgtgccgga aacgaatacg ggctccaaga tcgcaaccgg
     5881 ggtcaacttg ccagacttcc cgaccgaaac ttcgatctcg agaagcttcg tcggcttgcg
     5941 ctccggcgga aatttgtacg cgatgcccca gtgtgggtga tgccccgttg cgccgagccg
     6001 tgtgtagaaa ccaagctcgt cgatcttgat caccatgcca tcgatgtcgt agtcgagcga
     6061 ttcgcggcgc gcctgatagt cgtcgcagta ctgaagcgca gcctcgtccg aggtcgtttg
     6121 aaagacctcg ctggagtaaa cctcggcccc gcgatcggcg agccactgca aggtctcgta
     6181 ttgagtcgaa ggaagctcga ctccatccga ccaggcaact tgataaaaaa aggcgcgaat
     6241 gccggtctct tcgatgcccg tcggatcttt gcgtttcatc atcccggcac agccattgcg
     6301 cggattgatc agccgctttt cgagcgtcgc gttgaagcgc gtgaactcgc tgagcggcca
     6361 gtagagctcg ccgcgaatct cgagtgaccc cttgccgctc tcgcggaact tcgtcggaac
     6421 cgttccgagc tgggcgacct gctgagtgat gacatcaccc cggtggccgt cgccacgcgt
     6481 caccgcttgg cgcaggcgcc ccgcttcgta gagaagcgaa accgaaatcc cgtcgacctt
     6541 cggctcgacg atcaccggaa gcgcgtcgac ctcgagctct ttaaggcggc gccgaaacca
     6601 ggcggagagt tgctccgaga taggaatctg caccccggca ctgtcgcgcc gattcggcga
     6661 gagcttttcg agagacagca tcgggaccct gtgctcgacg gtctcgaaac cggccgtgtg
     6721 gtcctctccg ggctgctcat cgattcgctc ggccgcgtcg atcccgagta aatcggcgag
     6781 ctcctggtac cgttcgacga gctcatcgaa cgccgcatcc gggatctccg gagcccctgc
     6841 ccgatatgct gcctcgtgat accgaatctg ctgactgagc tgctccagct ctttcgcagc
     6901 ccgcgcccga tcgctcatcg cccgactttg ccagaattcc gccggtcgaa aaggcccgaa
     6961 ttgtcgtctt tttgggtgac cttgtgacaa cggggttcgg ctaaggcagc ttgtttaact
     7021 agggtatgga cgacgaaggg cagcgtcccg ggggtctcgg ggacggatcg atggctcggt
     7081 acgagcgcga gcgcgacgag cagacgcggc ttcgctcgct cggcgccctc tcgcacatgc
     7141 tgccagtgct ttcgttcatc tatgcggcgg cggccgcgct cttcctgctg gcaccgcctt
     7201 tcaccccgaa tcacccgcac gtcttcatct gcgccatttc ctcggcgctt gtgctgtgcg
     7261 tctgggcagg gatgcgcgtc gatgatccgc gtttcgacac cccgatctcc gtcgcgacca
     7321 tctcggtcgc cgcaggtatg ggactcacca tgttcgcggt cagcggtgcg atcagcaaca
     7381 ccacaacgct ggcgatcgcg atcgtggcag ccggtgcgct cctctttcga ttctcgatgt
     7441 atgcggtgat ggtcgcggtg gtgttcatgg gatggattcc gctggcctat ggctatgaaa
     7501 acggcgcggt gagcttgggc atattccacc tttgggcggc gaccgtcgtg ggtggtctgg
     7561 tcctctggtc gagacggcaa ctcatgttcg gtctccacga cgaggcccat gaagccgagg
     7621 ccatgcgcgc gctcgcaacc gagcaggcgc ggagtctcgc acgcgcccgt gatgccgcgt
     7681 tggcatccgc gcaggtcaaa ggtcagtttc tcgccaacgt gagccacgaa gtgcggacac
     7741 cgctcaacgg catcctcggc ctccttcaat tgatcgaccc gtccgcgctt ccaaaaccgc
     7801 aagacgagta cctgctcgaa gtcaacaagt cgagcggttc actcctcgcg atcgtcaatg
     7861 acctcttgga tctttccaag atcgaagccg gcgagatgcg cctcgaatcc gtagccttcg
     7921 acgtgatcgg catgaccgaa gagatcgcga tgaactacgc ttcggccgcg caggccaaga
     7981 gcatcgagct gatcacccag atcgccccgg atgtgcctac ggaggtctgc ggcgatccac
     8041 ttcgcctcag gcaagtgatg tccaacctcc tcaacaatgc gatgaaattc accaaggacg
     8101 gcgaggtggt gatcggcgtt cgcgtccgtg accgcggccc gtggcacgtc aacctcgagt
     8161 tcaggatctc ggacacggga gtgggcgtct cgaaggaacg agccgagagc atcttccgtc
     8221 cgttctcaca ggcagactcg tccacgacgc gtgagtacgg cggcaccgga ctcgggcttc
     8281 ccatctgccg ccaattggtc gagctgatgg gaggagacct tcagctcgaa agcaccctcg
     8341 gtcaggggag caccttcttt ttcgaagcgc gcttcgaatt ggaggagagg ctcagcgcgg
     8401 agctcaaggc cgtcactgcg gcgctctcgg gcatgcgagt tctcgtactc gaaggcaaca
     8461 accgggctcg cgagaccctt tgtgcacagc tcaccgcatg gaagatggag gctctggcga
     8521 cgacgacctg cgatggtgcg ctcggcgcgc tgcgcggaac cccagcgccg aacgtcgcgc
     8581 tcatcgacct ccgcagtctc ggagcggatt ggcggaaccg ggtttccgag ctcagcaagg
     8641 tggcgacgga gcgcggaaca tcggtcgtcg cggtctgttc acacaagcat gaacagagcg
     8701 aattgctcga atcgggcgtt cgcgtgcacg tcgagaagcc cattcgccgg acgaaaatca
     8761 tcgccgccct tctcgagacg ctgaagcggg caagcgtgcc tgtgcgctcc atcgaagagc
     8821 cccgaaaccc gtccttgcct cctcctcccc cgaaggacct cgtctcgaac ggcatgaaga
     8881 tcctcgtggc cgaagacaac tcgatcaacc agaaggtcgt tcgcgcccat cttcgagcgc
     8941 tgggctacga cgtcgacaca gtggacgatg gggcggccgc actcgaggct ctgaaggatg
     9001 gacacccgta cgctgccgtc atcatggacg ggcagatgcc gaacatggac ggctatgaag
     9061 caacgcgagc gcagagggcg cgagagctag agtcgggaca aggacgcgtt ccgatcatcg
     9121 cgctcaccgc ccatgcgatg acgggagacc gcaggactgc gttcgccgcc gggatggacg
     9181 actacctctc caagcccttc acgcagaagc agttgcagaa ggctctcgcc cgctgggcgg
     9241 cccggccatc ttcgagcctt tcggagtttc ctccggatgc cctcgactcc agcattacct
     9301 cacagctcct cgagctagag gaagaagagc cggggttcct ctgcgaagtc atccacagct
     9361 tctttcaaac cgctgaggac tgcatctccc ggatgagcaa ggccatcgac gccggtgatc
     9421 tcaagagcct gcgcgcggcc gcccacatgg ttcgcggaag cagtcagcag ctcggcgcga
     9481 gtcgcttcgg cgcaacctgc ttcaagctcg agcagctcga gtgcatggat gaagcgagcg
     9541 tgctcctcgg cgccctgaaa gatgacctcg aaggcgcgcg aggctcgctc acggaacttg
     9601 cagatcgtgc gctcgacgcc gcctcgtagc gggctgcgct cagtttcgct gcacgcctgc
     9661 gaggcgcgag gcgtccacta tccagctatc tcgaatcgtc tgctccggaa ctccgtcgac
     9721 gtgctcgacc agccactcca catccgatgg ctcgaagcgc gcgatctgat ggaagtgata
     9781 aatcccgaga cgattcagcg tgcgctctag actcgggtcg acgccgtcga ggcgctgaag
     9841 atcatcgcgg gcaccaaacg gctgaaccat caaccaagcg ggtggccgcg attcgcccgt
     9901 gcttcgaacc tcgagctcgg cttcacggat gcgtgccttg aggcgttcga tgtcgtccag
     9961 catgcgttcg tgaataccgt gcagaacttc gagctctgct ctcaatcgct cgccgcgggt
    10021 ctctgcctgc ctcaacgtag cgcgtgcgtc gtcgcgctcc tgcacggcgg tgcgaagctc
    10081 tcgtcgcaga tcttcggcgg actcgcgcga gcccggctgc gcctcgggtt ggatgaccga
    10141 aagccgcgta ctgacctcct gcatcgtccg attgcgcgcg tccagagtcg cggtgagctg
    10201 ccgtttcgca cgctccagcg cttcgatttt cgcgctcgag gcgctgagct ctttgtggag
    10261 cgctcgaatc cgctcgttga gcgtgatcat gtccgcgcgc gtttcttcga gctcgtactc
    10321 gagtgtcttg gcgcgaaccg gcgccaccaa acgcccccag aacacctgcc cgagccagag
    10381 aagcgccgca ccgagcaccg ccgccgccac caagctgccc accatctgca gcacgagata
    10441 cgtcacgttt cacctccccc aaggctttcg tcgtcggcaa cctgcaactg cgcgctcacc
    10501 actcgcacgc cacggaccat gctcacgatt cgctctgcgc gcagcgcctc cgacgcggaa
    10561 gcgacctcgc ctcggagctc gacgtcacgc ccgtccacga tcggaatgat gtcggaaaaa
    10621 ccggtgcgtt gaaggacgcc gctgacgcgc gttgccacgt cccactcgat ctcgtgccgc
    10681 atggggctga gcatggaaac ccagacgaca tagccgagga agcctgccga aaccgttcca
    10741 aagactgcct tcttccacat tgcgcggttt gcctacgagc cccattcaac tcgattggcc
    10801 gaaaaagatc cacgttctga cgaggcttcg gacctctcca aggcgatgca gcatacaaat
    10861 cccttcggtt catcgcccaa tcgggtgggc gctgcgagag ctcagcgctc gaagctcatc
    10921 tgctcgacga tccggaagag gcgttcttgg cggtcgtccg accaggtgta aagcgccacc
    10981 gacggtgcgt tgaacgtcac agcatcgagg tcgatcgcct tcgcccaagc gagcgccgcg
    11041 cgacgctcgg cagagtttgc gcgccgctga atgcgcgcaa gggtcatgtg aggaagcggc
    11101 ggacgatcgt cctcaggagc gcctgcggct ttcaagagcg gccctcgcgc ctcggcgatc
    11161 atctcgctca gctctttgcg cccacgatcg accatggcag agagcgctga gggcttccta
    11221 ggatggccga gcggctcgac ccccgtgaac gagcccgtca cccgccgaaa gctcggaaa
//
LOCUS       DASRZF010000122        21068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_428998, whole genome shotgun sequence.
ACCESSION   DASRZF010000122 DASRZF010000000
VERSION     DASRZF010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 21068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21068
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428998"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1221)
                     /locus_tag="VFG22_05645"
     CDS             complement(<1..1221)
                     /locus_tag="VFG22_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415758.1"
                     /translation="MGIMGQRSNLGLALALVAVLSAAVQPATAAAQGLDTDRANQEGP
                     GFKVGRLVLHPGFAATGGYDSNVFLQAANEEPSYILRLSGYLDVATEGAVRQSEGDSN
                     KSAPQKVEFRGGLGARYYHFFNGRIPVAVGGDAHVDFAYNPSKVFTLAVRDTFLRTVR
                     PFSNPNTAEGRTISYGRNLNVAALELVGRSKSQVLQGNLGYTNSFQFFDADVYGYGNN
                     VTHLVPASLSWLFFPTSAIVYEIEYAHQKYQDQAAIETSPTLLSNNNRVSNLFGYNGA
                     ITERFSLTAMIGYAAGFYTIGESFDDVIARADLRWKARPNIEVSTGFDRVVRPSFIGN
                     YAVVNRLHLDAQFILLGALQIGFKNWVSFDKSGLALNPDGSLLGNQPYRQDIRVNVNI
                     FAEYRFRSWLALVCD"
     gene            complement(1255..1854)
                     /locus_tag="VFG22_05650"
     CDS             complement(1255..1854)
                     /locus_tag="VFG22_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415759.1"
                     /translation="MFRRMGTVGQVLLAIFMVGGGLGYAVAQDSSEATVDVVEVEPMG
                     AAVEPVPSSPAAQAKAAKSAVASASASCDAQSAALQTAKRDKDIIRATCLDDKLSQCS
                     ANLQNIKRRQTALSEAIADGDTGRANHESTVIGVLSQKFKMLAQAANECVGQDLFDAG
                     DTQVKEQVDLFAPDEDPTVLTEVPDWPIPFIPPPVSGVS"
     gene            complement(1953..3404)
                     /locus_tag="VFG22_05655"
     CDS             complement(1953..3404)
                     /locus_tag="VFG22_05655"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HET6415760.1"
                     /translation="MTQAQTPRQGPRPGAMTMAMQSVPTKHSGPKVLRVGLFRDKKIV
                     EERIIRRRETVSIGTSAKNHLIIKTGTLPAKLESRFELFQLVGDDYILNFTPEMSGKV
                     ALPGGVQKLEHLRDTGAARDAGTHYQIKLADSSRGNIRVGDFTVFFEFVSAPPITPKP
                     QLPAAVQRGFVKNIDWTFTTWVIFSYMLFFGFIIYMENADWEVDRGAQEVPESLARLV
                     FEAPMEPPTVEEEIAEGEGEEEEAEAKPTEKKPSKAEGREPVEAEDAGASAEGRARIV
                     QEAAAQAEAMLLGALGGEGGALGDVLAGGAVTGNAADVLAQASGVGVATKGDAGALRT
                     RTGGGSGQQAGLGSLKKGKGVGKAAKEGEAVKERELRGNVNLNQGGDVGGSGEFDAAL
                     VQRQIKQRLKAITRCYESELRKNPSLAGKVTVTFTIQERGNVTNAQASENTTGSTAVA
                     SCVTRTISGFRFNPGPSGGSVTFRYPFVFQPQN"
     gene            complement(3404..3658)
                     /locus_tag="VFG22_05660"
     CDS             complement(3404..3658)
                     /locus_tag="VFG22_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415761.1"
                     /translation="MKRMASIALIALMFFGLSTAAALAQDAKKRQPRVIQLEEIIIEG
                     RVQKPNAFYILNRSNLGYEVLELRTSFVRKVVRSVQKEPF"
     gene            complement(3655..4719)
                     /locus_tag="VFG22_05665"
     CDS             complement(3655..4719)
                     /locus_tag="VFG22_05665"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6415762.1"
                     /translation="MSSKARRAFEQGVKAAADDDPGSAETSFQSALSRDPSAYPALYN
                     LGVLADRAGREGDAINYYRRALGILPDYEPAARGITTIQLRRGQVQEAIATVEPLANE
                     YRANIEMQALYAEVLVEARRLDEAWMAARRALKCNERFVPALIALVKASRAQGRDELA
                     DSILEQAMEVDPQVAELHFMSGEKLQAEPGRLREAMAAYERAIQLRPDYAEARMALGV
                     QLLAGGNYAGALAHFQAAERLVPTLPAVHVNLGDAYRAGSQWTEAQRAYRRALELQSK
                     LPEAHYGLGLMFLTAAGDFPGLDELSAYEKAVSELKTYRGEMGSRLARDDQSEEYLRD
                     LDRMIKRVRRQLEREEGGAS"
     gene            complement(4947..8804)
                     /locus_tag="VFG22_05670"
     CDS             complement(4947..8804)
                     /locus_tag="VFG22_05670"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6415763.1"
                     /translation="MIARLRLGSLLLLAALAVEALGGGMPNPASAQSSKGESGEDAVP
                     WDGAADVSLPPFLQDTEKAVVEERAPPTEQELEALRQLEEEVERYTDTGEAFRSTVGS
                     LVRRQYLQQRRERDRWYGEQLATEERAFSEARESSIQVFEEFVRRYPNHPKYTPDAMF
                     RLGELYFERSAVQFQAIYDKAQLARDAGDLSAEDHLPTSPDFSPTVVLYKSLARRFPE
                     YERSDGVYYLIGYTLNEMGRPEEAVAAWLALVCANKYRYDPEWRPAPPDASLAAQAKY
                     PALSLDGRPLGATVGGTFVDPYKSCRSITPEARFVSETWFRIGEFHFDDFGAENALDL
                     SIAAYGRILENPDDRNYNLALYKVAWAYYRASRYPEAIRHFAELVQWSDDTQRETGKA
                     GSELRPEAIEYLGIAFAYDDWNENQTADPIEGKPTGLERIQDASLLPQQRPWTPEVYF
                     QLGQVYFDEAKYPEAIATWRLTIRRWPNHHRTPEILNDIAVAHQMHNEFEEAIVARSE
                     LTQYVQGSEWWNANLDRPAEQRNAEQLAENALIVTALHHHQRAQRLRQQCLEDRNVRL
                     CRDSQVEYGLAATAYRSYLERYPNNPQGYELHYNLADALYWSKSYEQAATEYAEVRDS
                     NVDDSYLAESARRVVESLKRIAARDVEEGRLVVRDDAPAPGGAPPSVRPVAMPETVQR
                     LARAREIYLTRVPPQRDTEGVRAAYDYNNALLLYWYGYWPQAKARFSRIYEERCSGPQ
                     ADATGQIAWENLRAMAVAEDDSDEIRRLATEIQDRGCTFSAGAERIDCSLTGNRDKPI
                     CRAGADLNALVYQDALDVYKRAGQAQGTEQRMLYEQSATMLVAAVNSNPNDRQAPVAL
                     EYAALALEATNRFDSAAQLYQRIIDEDGPRKAEDEEEQKSLDAIVANAHFKLAYTASR
                     NFDFDRAVENYRVLADSQRFAQSTDPTVQSKRADGMVNSAILLERLQRYPQATEYYRR
                     VYTSVEDPATKRNALYRIAEMAYRQQRYPQAIAGMREFIGVYGNDGEAGDLVVQAHWR
                     IAESWKARGRAADYRKALVDVTTAFDRSGQPAGSVAAGYAAEARFILVDGKLASFERF
                     EIKPGKPKTVEAYINVIEKQIGSGAKEAQSIVEGYEPVFPYRRPRWTIASYVRQGRVY
                     EILARSILNTPVVIPLDLKNALRRAGPDERQEYELDFDDKVRQVLDSQVRPVECFAVA
                     RYALAARAGRAGSFDDEYTRIAIDRLQAYGDERIAECIAEAQGRDATFQGYVPGEFVR
                     SPRGKPMEIRPGIAPPAINKGVE"
     gene            complement(8801..11155)
                     /locus_tag="VFG22_05675"
     CDS             complement(8801..11155)
                     /locus_tag="VFG22_05675"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6415764.1"
                     /translation="MTGRGRARGVWLGLILASSLFAFDVPEAAAQDMGAASRELADLE
                     SDTQRLKADRVRAQNLKSDTFVEERLADGELYLRLEDYLRAAILFTDIVDHYPAHRAY
                     PEALFLLGESLFHAGDYLGARRRYAEVAERSGEPAFAPYLQMSLSRLIEIAIHTRDFR
                     EIDAYFGRLEALPSSELSVTMAYFRAKYLYNRAVPIDEVLNAPPSAALRGVDQARLER
                     ARKGFLAIADGGEYSLRAKYFVGTIYALRGEYLDAIAAFRGVLGLEPANQLEAKVVEL
                     TYLALGRLYYETEQLEQAAEAYRAVDQASTHFDTALFELAWTYIRMGDAIQAERALEV
                     LSVAAPDSPLNADGKVLRGDLLARSGRYEEAEIVFDEVRDTFGPIRDDLERTRLEHPD
                     LHAHFRAIVRENLDSFDLDDFLPPSARRWIDLEDDYERALEVLADLSEAKQLVAETDE
                     LAQRITAALSAPNGVSVFSDLRRQRERSTGLRNRAARTRGALISREASLRGNRQGEVA
                     RVRSRREELQATVMKMPVDTEEFVDRDLVKLEEYRAAERQLQGLRVEILGLEARIVAS
                     RTGMAAVDPEKVDPEALRQQLAEHEAEVRDFEAQLTWIRRRLEVGRLHVGVGDKRYQA
                     DDAEREKFIDLVTRERQLAGSGGPQYDDAFARVASIERQLDQRDAEIAAMVRRRVAEM
                     LVVVDEETANLVRYRKALSSLEGETEDVVGAITYLNFNRIHQRFYELVLRADVGKIDV
                     SWAKREDRRLRLDVLTRERARELQALDDEFRDVMDQSQGSEGGP"
     gene            complement(11194..11901)
                     /locus_tag="VFG22_05680"
     CDS             complement(11194..11901)
                     /locus_tag="VFG22_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415765.1"
                     /translation="MRRTVALSVVAACILAGSAIAAAQAGGTPPTDPSSEAAAATGTS
                     QAAASKPIDAGTYAVRLRDLEQRINELKEQIFRSKARLSLLAETVLEGVVGGGQAVIL
                     HENRMSQSYKLVRVVYALDGAPIFTKADEEGTLGEQQEFEVYNGSIVPGDHTLTVNLE
                     YRGHGYGVFAYLKGYRFKVRSTYTFTVPEGRISTVHVVGYEKGGPTTPLEERPDVRYI
                     ERVERQVRLDASGEGDR"
     gene            12152..12739
                     /locus_tag="VFG22_05685"
     CDS             12152..12739
                     /locus_tag="VFG22_05685"
                     /inference="COORDINATES: protein motif:HMM:NF009166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET6415766.1"
                     /translation="MSDPSDEALLAAYRSGESRAFEILLGRYRGPLFNFLLRSSRDRG
                     RAEELYQDVWMKVIERCDDFRGDAKFSTWLYAIARNLAIDHRRKMKFRGHAPLDATQP
                     RTGQSIGERLGNPGPSTEQLAIGGLIRARIGDAVEELPEEQREVFLLRQLQGLAFNEI
                     AEVVGVPANTVKSRMRYALERLQGLLSDLEESDEL"
     gene            12729..13592
                     /locus_tag="VFG22_05690"
     CDS             12729..13592
                     /locus_tag="VFG22_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415767.1"
                     /translation="MNCDECKEQIFELIEQETIDPDGVRALLARCPDCHSLFDETKAA
                     LRTAAMLPLEEPPLSIDAAIVRAAAERVPTQRPSGWRRLQAAPWAMAAVALLALGIGV
                     WTLPRGGELEGSDGFVGKREAHGELIARDELGDASSQEPVIAALEPAPARAIGGAEAD
                     APATGLETASSRSARAKQRAGAPVAPAVARQVAGARKASPEAAAPRLAAQERKEDAIA
                     ACQRRVAEHEGAEQDEADATIDAEQALALGECYQKLGKKTKARRWLTRAAGEPVTKLR
                     AEQALRGLDAE"
     gene            complement(13598..14374)
                     /locus_tag="VFG22_05695"
     CDS             complement(13598..14374)
                     /locus_tag="VFG22_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415768.1"
                     /translation="MIERAKPPTGRVARADLLTEAAPLIPLLMELPADVLAGGDYASF
                     QFRECFTVLSLLGRRLALLDLTPSTAIEVVRLILASTEGEANPLPTSFSQRAVEAAVE
                     GFVLGREERVTEASEARAARHLKPLRIDESAFALILTGVHEPAVFGDCVDALGRAMLD
                     TGIEIAIVDLSQLGEPNGERVAAILAADEIARMLGGVCIFSGIDSRWRAAARDAGIEL
                     DALQIAPNLGDALASARATSQRTAKDRKLNWRGLIERLRR"
     gene            14477..15874
                     /locus_tag="VFG22_05700"
     CDS             14477..15874
                     /locus_tag="VFG22_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6415769.1"
                     /translation="MAATILVVDDEKNIRRTLRMVLEGSGFNTLEAESAEECLETLGR
                     DEVDLVILDVRLPKMSGIEALQKIRNEPETRKLPVLMVSGHASLAEAVQAVQLGATDF
                     FEKPLDRDRILLTVRNALKTWQLQREVEQLRADVEHRYEMIGESEEMANLFAQLEKVA
                     PTNGRVLISGESGTGKELIARAIHRLSPRADKPFIKVNCAAIPSELIESELFGYERGA
                     FTGAQGRKKGMFELANGGTIFLDEIGDMSASAQAKVLRALQSGEISRVGGEKTIAVDV
                     RVLAATNKDLELEVRDGRFREDLYFRLNVVPIVSPPLRDRRQDIPLLAKGFLREFSIE
                     NGLREKPIDEGVLEALAERPWPGNVRELRNVVERMAILSGDRITLDDLPEEGVLSEVR
                     RESAAPSDLPSRRDETGERLTLRDYRTRAEKNYIMETLDEVGWNISRAATILGVERTN
                     LHKKMRSYGIRRDEP"
     gene            15871..16215
                     /locus_tag="VFG22_05705"
     CDS             15871..16215
                     /locus_tag="VFG22_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415770.1"
                     /translation="MIRRSLRLMWTGAVLGFLAYVLFFVHLGERTTFEHLMRVINTEE
                     AKELGRDLGAATARLSKQIGDQVHDATKPPAQDAGTSQVPEVVSDKLQGMVGHSYERD
                     ERMETALQNDAQ"
     gene            complement(16222..17577)
                     /locus_tag="VFG22_05710"
     CDS             complement(16222..17577)
                     /locus_tag="VFG22_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6415771.1"
                     /translation="MARILIVDDQDSARRMLSEILQAEGYAVDQAPNGESACHMVAES
                     GYDLVITDLRMGDVGGLEVLQRTRESAPLTEVIVMTAFGTIEDAVEAMRFGARDFVQK
                     PFVEEELLIKVARAARTRQLAGERRSVGDDFRERYNFGNIIGQSAEIRDILGRIVRIA
                     PTDATVLITGESGTGKELVARAIHANSLRAESPFVSINCAALTETLLESELFGHMRGA
                     FTGAVQTRKGLFEEANGGTFFFDEIAETPPSLQAKLLRAIQEGEIRRLGDNKPINVDA
                     RIIAATNQNLRNLIEEKRFREDLYYRLNVARFELPPLRERKEDIAPITESFLQKYGKK
                     MGRPAVLGPGVMDHLRGYDFPGNIRELENLIEQGVALAQDGKVQLSDILPNSNGNGNG
                     RVSHPGRQSLADIVQDVEREAIVRALQHSAGNKERAAELLGLSPTTLWRKMKRLQLEW
                     P"
     gene            complement(17639..18937)
                     /locus_tag="VFG22_05715"
     CDS             complement(17639..18937)
                     /locus_tag="VFG22_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET6415772.1"
                     /translation="MILRDPVHGLIAFEGMAERVIRSLLDTREVQRLRRVRQLGLASL
                     VFPGAEHTRFSHAIGTAHVMQSLLHRIDACSDELPSDQRLDEEARSEALAAALLHDLG
                     HGPFSHLFEEVLPHAKHHELWTQEILLDPATEVHQALESISEGMSDRVTSLLAGNYRL
                     GYLSQSVSGTLDVDRADYLLRDSHMTGVRYGLYDLDWLLQALTFACVDGRWVLAIQGR
                     KGLPPAESFFLGRHFMYQQVYHHKATRAAEALVRAIFMRVSELIFAGTPPTPLLPAFR
                     AAALGESLSLGEYLRMDDAELLSCLSAWEHGGDPTLAELSKRLRCRALPKTVPLRENR
                     RDQWDEALARAREIVAAGGLRTDLTVWLDIAEDVPYPEPTDGSTDGLWVTLRHQPLQR
                     LGEASFILGQLRNKRIERPRLIFPSEFRSQVLAAMHDVLD"
     gene            complement(18960..20444)
                     /locus_tag="VFG22_05720"
     CDS             complement(18960..20444)
                     /locus_tag="VFG22_05720"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6415773.1"
                     /translation="MIGFGCNRPVADEADALVVMLPRDAEQLDPRFVSDPYGLRVTRL
                     LFASLVTIDPRTLEVIPDLAESVKAMSATEFEVRLRDGLTFSDGSALDAEDVVATFEG
                     VVDPALRSRYAHSYRRIAEMRVLDSRTIVFVLHEPHATFITDLELPIVRSEDAFRRIA
                     TPEDPLPIGAGPYRLVKRQAGVLELRANPHWHRGKTKYPSLRLVVIRDDNTRALRMLA
                     GEGDIAIASIPPLLLPLFQDDPRFEIRSAAGISTMYLGFQTRSTKLSDVRVRQAIGYA
                     IDRELLVQSKYEGLARVTDSWIPHGHWAEARATPPRAFDPEKARALLDAAGFSDPPGS
                     EPRMRLALRTTSERFRQSVARAIAAMLRDVGIEVDVRPSEAATLISDLNRGQFEITLL
                     QVPEVIEPHVLSWFFASSRIPGPDAEGANRWRYANEELDGLFETGRTEIDRDRRRAAY
                     DEVQRILARDVPVLPLWQPDTVAVVRQGSTFEVPRDGRFGTLAF"
     gene            20481..>21068
                     /locus_tag="VFG22_05725"
     CDS             20481..>21068
                     /locus_tag="VFG22_05725"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HET6415774.1"
                     /translation="MRYRGLILIAIICLLVASPGADAKEPAPLAQTIASLVRQAGLGD
                     GLGIHVVRLQDSEELYRNQPERPRNPASNQKLLTSAAALWRLGPAFRATTKVEGIIEN
                     GRVATMVIRPSGDPSLGYAGLDALAESLRLRGVDTVDRILIDDSYFDQQILPPAFEQQ
                     PHEAASFRAAISAFSVERNSYVVHIGPGPKVDAPGR"
BASE COUNT         3744 a         6554 c         6661 g         4109 t
ORIGIN      
        1 atcgcaaacc agtgccagcc acgatctgaa gcgatactcg gcgaagatgt tcacgttcac
       61 gcgaatgtct tggcggtagg gctggtttcc gaggagcgag ccgtcgggat tgagcgcaag
      121 cccagacttg tcgaaggata cccaattctt gaatccgatt tggagggcgc ctaggagaat
      181 gaactgcgcg tccaaatgca ggcggttgac gactgcgtaa ttgccgatga acgagggacg
      241 aacgacccgg tcgaatccgg tgctgacttc gatgttgggc cgcgctttcc aacgtaagtc
      301 ggcgcgggcg atcacgtcgt cgaagctttc gccgatcgtg tagaaccctg ccgcgtatcc
      361 gatcatcgcc gtaagcgaga atcgttcggt gatcgcgccg ttgtaaccga acaagttcga
      421 gacgcgattg ttgttggaga gcaaggtggg ggaagtttca atcgctgctt ggtcttgata
      481 cttctggtgg gcgtattcga tctcatagac gatggccgac gtgggaaaga acagccaact
      541 cagtgaggcg ggcacgaggt gcgtcacgtt gttgccgtat ccgtagacgt cggcatcgaa
      601 gaattggaac gagttcgtat agccgaggtt tccttggagc acctggctct tgctacgtcc
      661 cacgagctcc agggccgcga cgttgaggtt gcggccgtag ctgatggtgc ggccttccgc
      721 ggtatttgga tttgagaacg gtcggaccgt gcgaagaaac gtatcacgga ccgccagcgt
      781 gaagaccttc gagggattgt aggcgaagtc gacgtgcgca tcgcctccga cagcgaccgg
      841 gatccgcccg ttgaaaaagt gatagtagcg tgccccgaga ccaccgcgga actcgacctt
      901 ctgcggtgct gacttgttgg aatctccttc gctttggcgc acggcgccct cggtggcgac
      961 gtctagatag cccgataggc gcaggatgta ggagggttct tcgtttgcag cttggagaaa
     1021 gacgttcgag tcgtagcccc ccgtggcggc gaaacccgga tgtagaacca agcgtccgac
     1081 cttgaaaccc ggtccctctt ggttcgcgcg gtcggtatcc aagccctgcg cggccgccgt
     1141 tgccggttgc actgcagcgc tcaggaccgc cacaagagcc agagcaagcc ctaagttcga
     1201 gcgctggccc atgatcccca tttcccccca tacatgctga caccgttccg cgatttagct
     1261 gactccactc acgggagggg ggatgaatgg gatcggccag tcgggcactt ccgtgagcac
     1321 cgttgggtct tcgtctggag cgaagaggtc gacctgctcc ttcacctggg tgtcacctgc
     1381 atcgaagagg tcctgaccca cgcattcgtt cgcagcctgc gcgagcattt tgaacttctg
     1441 cgagagcacc ccgatgacgg tggactcgtg attcgcgcga ccagtgtccc catcagcgat
     1501 ggcttcgctg agggcggttt gccggcgctt gatgttctgc agattggcgg agcactggct
     1561 gagcttgtcg tccagacagg tcgcccggat gatgtccttg tcgcgtttcg cagtctgaag
     1621 cgccgccgat tgagcatcac agctcgcgct cgcgctcgcg acggcagact tagcggcctt
     1681 ggcctgcgcg gccggcgacg acggcaccgg ctcgaccgca gcccccattg gctcgacctc
     1741 gaccacatcg accgtagcct cgctcgagtc ctgagcgacg gcgtaaccta gccctcctcc
     1801 gaccatgaaa atggccagga gaacctgccc tacggtcccc atccgtctaa acatcaacga
     1861 tcccctaaac ttgcggcgat tatacgctgc accatactcg tgtcaatgga tccataatac
     1921 agttaagcag taaaactatg atctagtcga agctaatttt gaggctggaa aacaaaggga
     1981 tagcggaagg tgacgcttcc gccacttgga cccgggttga atcggaagcc gctgatcgtc
     2041 cgggtcacgc agctcgcgac ggcagtgctg cccgtcgtat tctcgctggc ctgggcgttt
     2101 gttacgttgc cccgctcctg gattgtgaag gtcacggtga ccttgccggc gaggctgggg
     2161 ttctttcgaa gctcggactc gtaacagcga gtgatggctt tcaagcgctg tttgatctgc
     2221 cgctggacca gagcggcgtc gaactcgcca ctgcctccca cgtcgccgcc ctggttcagg
     2281 ttcacgttgc cgcgtagctc gcgctccttg acggcttcgc cctccttggc tgctttcccg
     2341 actcccttcc ccttcttgag tgagcccagg cctgcttgct ggccgcttcc cccgccagtg
     2401 cgcgttcgca acgcaccggc gtcgcccttg gtcgccacgc cgacgcccga agcctgcgcg
     2461 agcacgtcgg ccgcgtttcc ggtgacggcg ccaccagcca gcacgtctcc gagcgcgccg
     2521 ccctctccgc cgagcgcgcc gagcagcatc gcttcggctt gggctgcggc ttcctgcacg
     2581 atgcgcgcgc ggccctcggc actcgcgcct gcatcctctg cttcaacggg ctccctgccc
     2641 tcggccttgg acggcttctt ctccgtaggc ttggcttcgg cctcctcttc ctctccctcg
     2701 ccttcggcga tctcctcttc cacggttgga ggctccatcg gcgcttcgaa aaccaaacgt
     2761 gcgaggcttt caggcacctc ctgcgcaccc ctatccacct cccaatctgc gttctccatg
     2821 tagatgatga atccgaagaa cagcatgtac gaaaagatga cccaggtggt gaaggtccag
     2881 tcgatgttct tgacgaagcc ccgctggacg gcggcaggaa gctgcggctt gggcgtgatt
     2941 gggggcgccg agacgaactc gaaaaagacg gtgaagtccc ccacgcgaat gttgccgcgt
     3001 gagctgtccg caagcttgat ctgatagtgc gtgcctgcgt ctcgcgcagc cccggtgtcg
     3061 cggaggtgct cgagcttctg aacgccaccc ggcagcgcga ccttgcccga catctcaggt
     3121 gtgaagttga ggatgtagtc gtcgccaacg agctggaaga gctcgaaccg agactcgagc
     3181 ttcgctggca aggtgcccgt tttgatgatc aggtgattct tggcagaggt cccgatcgag
     3241 actgtctccc gccgccgtat gattcgctct tcgacaatct tcttgtcccg aaacagcccg
     3301 acccgcagca cttttggacc cgaatgcttc gtaggaaccg actgcatcgc catcgtcatg
     3361 gctccgggtc gcggtccttg cctcggcgtt tgtgcttgcg tcatcagaac ggttccttct
     3421 gtacgctgcg aactaccttc cggacgaagc tcgttcgaag ctcgagaact tcgtaaccga
     3481 ggttggatcg gttcaggatg tagaacgcgt tcggcttctg cactcggcct tcgatgatga
     3541 tctcttcgag ttggatgacc cgtggttggc gtttcttggc gtcctgcgcc aaggctgcag
     3601 cggtcgatag tccgaagaac atgagcgcga tcagggcgat cgaagccatc cgtttcacga
     3661 ggcacctccc tcttcgcgtt cgagctggcg gcggacgcgt ttgatcatcc gatccaagtc
     3721 acgcagatac tcctccgact gatcgtctct tgccagccgc gaccccatct cgcctcggta
     3781 ggtcttgagc tccgagaccg ctttctcgta tgcgctgagc tcgtccagac cggggaaatc
     3841 accggcggcg gtcaggaaca tgagccccag cccgtagtgg gcctcgggaa gcttcgattg
     3901 cagctccaga gcgcgtcgat aggcccgctg cgcttctgtc cactggctgc ccgcccggta
     3961 tgcgtctccc aagttgacgt gtaccgcggg aagcgtgggg accaagcgct ccgccgcctg
     4021 gaaatgcgcg agcgcgccag cgtagttgcc gccggcgagg agttgaacac ccaacgccat
     4081 tcgagcttct gcgtagtccg gtcggagttg gatcgcgcgt tcgtaagctg ccatcgcctc
     4141 ccgcaagcgg ccgggctcgg cctgaagctt ctcgccgctc atgaaatgaa gctccgcgac
     4201 ctgcgggtcg acctccattg cctgttccaa gatcgaatcg gcaagctcgt cacggccctg
     4261 cgcacggctg gccttcacca acgcaatcag cgcgggcacg aagcgctcat tgcacttcag
     4321 ggctcgtctt gctgccatcc aggcctcatc gagccgtctc gcttccacca ggacctctgc
     4381 atagagggcc tgcatttcga tgttggcgcg gtattcgttg gcgagtggct ccaccgtcgc
     4441 gattgcttcc tgaacttggc cgcgccgaag ctggatcgtc gtgatgcccc gcgcggcggg
     4501 ctcgtagtcg ggaaggattc cgagagcccg acggtagtag ttgatcgcgt cgccttcgcg
     4561 tcccgcgcgg tcggccagta cgccgagatt gtacagcgcc gggtaagcac tcgggtccct
     4621 gctgagagcc gattgaaagc tcgtctccgc cgagccgggg tcatcgtctg cggccgcctt
     4681 gacgccttgc tcgaaggccc gtcttgcctt ggacgacatg tcgggcggcg acgggcgccg
     4741 acactgctcg gcgtgagtgg caccccaagg gcttgcttgc cccgcgtcga agtccgcgcc
     4801 ggcgcttgcc tctgatgagc cgttgggtga ttcccctcct gcagtagcct cggcgttacc
     4861 gccattggat gcagattgcc ccttgcaccc gtgtgtgcac agaagcatcg ctccgatcaa
     4921 aacagccgtg ggcgcccgca gaagcatcat tcgaccccct tgttgatagc aggtggtgcg
     4981 attccaggtc gaatctccat cggcttgcct ctcggagagc gaacgaactc ccctggcacg
     5041 tagccctgga atgtcgcgtc gcggccctgg gcctcggcaa tgcattccgc gatccgctcg
     5101 tcgccgtagg cctgtaggcg gtcgatcgcg atccgcgtgt attcatcatc gaagctgccg
     5161 gctcggcccg cccgcgcagc aagggcgtat cgcgcgacgg cgaaacattc gaccggacgc
     5221 acctgactgt ccagcacctg gcgaaccttg tcgtcgaaat cgagctcgta ctcctggcgc
     5281 tcgtcgggac cggcccttcg aagcgcgttt ttcagatcga ggggaatgac gacgggcgta
     5341 ttcagaatcg agcgtgccag tatttcatag acgcgaccct ggcggacgta cgacgcgatc
     5401 gtccagcgcg ggcggcggta tggaaacacc ggctcgtagc cttcgacgat cgactgtgcc
     5461 tctttggcgc cgcttccgat ctgcttctcg atgacattga tgtaggcctc gactgtcttt
     5521 ggctttcctg gcttgatctc gaagcgctcg aagctcgcaa gcttcccatc cacgagaata
     5581 aaacgcgcct ctgccgcata gcccgccgcg accgatcctg cgggctggcc gctacggtcg
     5641 aatgcggtcg tgacgtcgac gagagccttc cgataatcgg ccgctcgacc acgtgctttc
     5701 caggattcgg cgatcctcca gtgggcctga acgacgaggt cgccggcctc cccatcgttg
     5761 ccgtatactc cgatgaactc gcgcatcccg gcaatcgctt gtgggtagcg ctgctgccga
     5821 taggccatct ctgcgattcg atagagcgcg tttcgcttcg ttgcagggtc ctcgaccgaa
     5881 gtgtagacgc gccggtaata ctcagtcgct tgggggtacc gctgtaaccg ttcgagcagg
     5941 atcgcggagt tcaccattcc gtctgcgcgc ttgctctgaa ccgtgggatc ggtcgactgg
     6001 gcgaagcgct gcgaatcggc caggacgcga tagttctcga cggcacggtc gaaatcgaag
     6061 tttcggctcg cggtgtacgc aagcttgaag tgtgcgttgg cgacgatcgc gtcgaggctc
     6121 ttctgttctt cttcgtcttc tgctttgcgc ggcccatcct catcgatgat ccgctgatag
     6181 agctgcgccg cagagtcgaa gcggttggtg gcttcgagcg cgagggccgc gtactcgagc
     6241 gcgactggcg cctgccggtc gttggggttg ctgttgaccg cggcgaccaa catcgtggcg
     6301 gactgctcgt agagcattcg ctgttcggtt ccctgtgcct ggcccgctcg cttgtagacg
     6361 tcgagcgcgt cctggtagac gagcgcgttc aagtccgcgc cagcgcggca aatcggcttg
     6421 tcccgattcc ctgtcaggct gcagtcgatc ctttcggctc ccgcggagaa ggtgcagccg
     6481 cgatcctgaa tctcggtcgc cagccggcgg atctcatcgg agtcgtcttc cgcgaccgcc
     6541 atggcgcgaa gattctccca ggcgatttgg cccgtcgcgt cggcctgcgg acccgaacag
     6601 cgctcttcgt agatgcggct gaagcgcgcc ttggcctggg gccagtaccc ataccagtag
     6661 agaagcagcg cgttgttgta gtcgtaggcc gcccgcacac cctcggtatc gcgctgaggt
     6721 ggaacgcgtg tgaggtagat ttccctggcg cgcgccagcc gctgaacggt ttccggcatc
     6781 gccaccggcc gaaccgaggg cggggcgcca cccggagcag gtgcatcgtc gcgaacgacc
     6841 aagcggcctt cctcgacgtc gcgagccgca attcgtttga gcgattccac gactcgccgc
     6901 gcggactccg cgagatagct gtcatccacg ttcgaatctc ggacctcggc gtactcagtt
     6961 gcagcctgct cgtagctctt cgaccagtag agcgcatcgg cgaggttgta gtgcagctcg
     7021 tagccttgcg ggttgttcgg ataacgctcc aagtagcttc gatacgcagt tgcagccaag
     7081 ccgtattcga cctgcgagtc tcggcacagc cgcacgtttc ggtcctcgag acactgctgt
     7141 cgcaagcgct gtgcgcgctg atggtggtgc agcgcggtca cgatcaacgc gttctcggca
     7201 agctgttcgg cgttccgctg ctctgcgggg cgatccaagt tggcgttcca ccactcgctt
     7261 ccctgaacgt actgcgtgag ctcggagcgc gcgacgatgg cttcctcgaa ctcgttgtgc
     7321 atctgatgtg cgaccgcgat gtcgttgaga atttcgggag ttcggtggtg gttcggccac
     7381 ctgcggatgg tgaggcgcca ggtcgcgatg gcctccgggt acttcgcctc gtcgaaatag
     7441 acttggccga gctggaagta cacctcgggc gtccaaggcc tttgctgggg tagcaaagac
     7501 gcatcctgaa tgcgttcgag gcccgtgggc ttgccttcga tgggatcagc ggtctggttt
     7561 tcgttccagt cgtcgtacgc gaacgcaatg ccgaggtatt cgatcgcctc gggtcgaagc
     7621 tccgaacctg ccttgccggt ttctcgctga gtgtcgtccg accactggac gagctccgcg
     7681 aaatgccgaa tcgcttcggg ataccgactt gctcgatagt acgcccaggc cactttgtag
     7741 agcgcaagat tgtagtttcg gtcgtcggga ttttcgagaa tgcgtccgta cgccgcgatt
     7801 gaaagatcga gcgcgttctc ggcgccaaag tcgtcgaagt ggaactcgcc gatacggaac
     7861 caggtttcgc tcacgaagcg ggcctctggt gtgatcgacc ggcagctctt gtagggatcg
     7921 acgaaggttc cgccgaccgt tgccccaagc ggcctgccgt ccaggctgag cgctggatac
     7981 ttggcctgag cggcgagcga tgcgtcgggc ggcgccggtc gccattcggg gtcgtatcga
     8041 tacttgttgg cgcagacgag cgcgagccag gcggccaccg cttcttccgg acggcccatc
     8101 tcgttgagcg tgtagccgat caaatagtaa acaccgtccg agcgttcata ctcggggaag
     8161 cgtctcgcca agctcttgta gagcacgacc gtcggagaga agtccggaga cgtcgggagg
     8221 tgatcctctg cgctgaggtc gccggcgtct ctcgccagct gtgctttgtc gtagatggcc
     8281 tggaactgga cggcgctccg ttcgaagtag agctcgccca aacggaacat cgcatccggc
     8341 gtgtatttcg gatggttcgg gtagcgccga acgaactcct cgaatacttg gatcgagctc
     8401 tctctcgctt cgctaaaggc gcgttcttcc gtggccagtt gctcgccgta ccagcggtcg
     8461 cgttctctgc gttgctgcaa gtattggcgc cgaaccagtg agccgacggt cgatcggaat
     8521 gcctctccgg tatcggtgta cctctcgacc tcttcttcga gctggcgaag cgcttcgagc
     8581 tcctgctccg tcggaggcgc ccgttcctcg acgaccgctt tctcggtgtc ctgaaggaat
     8641 ggcgggaggc tcacgtcggc cgcgccatcc caggggaccg cgtcctcgcc cgattcaccc
     8701 ttgctgctct gcgccgatgc gggattcggc atcccccctc cgagcgcttc cacggcgagc
     8761 gcggcgagca gcaagagcga gccgagacga aggcgagcta tcatgggccc ccctcgctgc
     8821 cctggctctg atccatcacg tcacggaact cgtcgtcgag tgcctggagc tctctggctc
     8881 gctcgcgggt caagacgtcg aggcgaagtc tgcggtcttc gcgcttggcc caagacacgt
     8941 cgatcttgcc aacgtccgcg cgcagcacca gctcgtagaa ccgttggtgg atgcgattga
     9001 agttgagata cgtgatcgcg cccaccacat cttctgtttc gccctccagc gagctgaggg
     9061 ctttgcggta tctcacgagg ttggcggtct cttcgtcgac cacgacgagc atctccgcga
     9121 cacgtcttcg taccatcgcg gcgatctctg cgtcacgctg atcgagttgt cgctcaatcg
     9181 acgccacgcg ggcaaaggcg tcgtcgtatt gtggaccccc cgagccggct agctgacgtt
     9241 cccgggtgac caggtcgatg aacttctctc gctccgcgtc gtccgcttgg tatcgcttgt
     9301 ctcccacgcc gacgtgaagc ctgccgactt ccagcctgcg acgaatccaa gtgagctgtg
     9361 cctcgaagtc tctgacttcc gcctcgtgct ccgcgagctg ctgccgcagc gcctcgggat
     9421 cgaccttctc gggatcgacg gcggccatcc cggttctcga tgcaacgatg cgggcctcga
     9481 gcccgaggat ctcgacgcgc aagccttgaa gttgccgctc tgcagctctg tattcctcga
     9541 gcttcacgag gtctctgtcg acgaactctt cggtgtcgac tggcatcttc atgacggttg
     9601 cttgaagctc ctcacgtcgc gagcgaaccc gcgccacctc gccttggcgg ttaccacgca
     9661 gacttgcctc gcgggagatc agcgcgccgc gcgtgcgggc tgctcggttt cggagtccgg
     9721 tgcttcgctc gcgctgccgg cgaaggtccg agaagacgct gactccgttg ggtgcgctga
     9781 gggcagcggt aatgcgctga gcgagctcgt ccgtctccgc cacgagctgc ttcgcctcgc
     9841 ttagatcagc gagcacctcg agcgctcgct cgtagtcgtc ttcgaggtcg atccagcgcc
     9901 gcgcggatgg cgggaggaag tcatcgaggt cgaagctgtc gagattctcg cgaacgatgg
     9961 cgcggaaatg cgcgtgaagg tccgggtgct ccaggcgcgt gcgttcgagg tcgtcgcgga
    10021 tgggcccgaa tgtatcgcgc acttcatcga atacgatctc ggcctcttcg tagcgtccgc
    10081 ttcgggccag aagatctccc cgaaggacct tgccgtccgc gttgaggggg ctgtcgggtg
    10141 cggccaccga aaggacttcc agagcccgtt ccgcttgaat cgcatcgccc atcctgatgt
    10201 aggtccaggc gagctcgaaa agagccgtgt cgaaatgcgt cgaggcctgg tcgacagcac
    10261 gatacgcttc ggcagcctgc tccagttgct cggtttcgta gtagagcctg ccaagggcga
    10321 ggtacgtgag ctccacgacc ttggcttcga gctggttggc cggctcgagt ccgagaacgc
    10381 ctcggaatgc cgcgatggcg tcgaggtatt cgccacgcaa cgcatagatc gttccgacga
    10441 agtacttcgc gcgcagcgag tattcacccc catcagcaat cgcgaggaat cccttgcgtg
    10501 ctcgctcgag gcgcgcctga tcgaccccac ggagggcggc gctcggagga gcgttcagca
    10561 cttcgtcgat cggcacggcc cggttgtaga ggtacttcgc gcggaagtaa gccatcgtca
    10621 cagaaagctc tgaagacggc aatgcctcga ggcgcccgaa gtatgcgtcg atctcccgaa
    10681 agtcgcgcgt gtggattgcg atctcgatca aacggctgag cgacatctgc aggtagggtg
    10741 cgaaagccgg ctcgccgctc cgctccgcga cctcagcgta gcgacgtcgc gctccgagat
    10801 agtcgcccgc atggaatagc gactccccaa ggaggaacag agcctctggg tacgcccggt
    10861 gcgccggata gtggtcgacg atgtcggtga aaaggatggc cgctcgaaga tagtcctcga
    10921 ggcgcaggta gagctctccg tcggctagtc gctcttctac gaaggtgtcg ctcttgagat
    10981 tctgcgctct gacgcgatcg gccttcaagc gttgagtgtc cgactcgagg tcggcgagct
    11041 cgcggctggc ggcgcccata tcctgcgcag cggcctcagg cacatcgaac gcaaaaaggc
    11101 tcgaagccag gatcaacccc agccaaaccc cacgcgctcg ccccctgcca gtcatcggtg
    11161 tgggcggcga cctggtcgcg ccacgagctt gcattaccga tcgccctcac cgcttgcatc
    11221 gagtcgaact tgacgctcga cgcgttcgat ataccgaacg tccgggcgct cctcgagagg
    11281 agtcgttgga ccgccttttt cgtaacctac gacgtgcacg gtcgagattc gaccctccgg
    11341 gacggtgaag gtgtacgtcg accggacctt gaagcgatac cctttgagat aggcgaaaac
    11401 cccgtagccg tggccacggt attcgaggtt gacggtcaag gtatggtcgc ccggaacgat
    11461 gcttccgttg tagacctcga attcctgttg ctccccgagg gtcccctcct catcggcctt
    11521 cgtgaagatc ggcgcgccgt cgagcgcata gaccacccgc acgagcttgt acgattgcga
    11581 catgcggttc tcgtgcagga tgaccgcctg cccacctccg acgacgccct cgagcaccgt
    11641 ttcggcgagc agcgaaagcc tggccttcga acggaagatc tgttctttga gctcgttgat
    11701 tcgctgctcg aggtcccgaa gccgcaccgc gtaggtgcct gcatctatcg gcttgctcgc
    11761 cgccgcctgg ctcgtaccgg tggcggcggc cgcctcgctt gacgggtccg ttggcggtgt
    11821 cccgccggcc tgcgccgctg cgatggcgct ccctgccaag atgcacgccg ctacgactga
    11881 aagcgcaact gtcctgcgca tcgcaattcc cctctaagtg accaccatct gaagcgaaat
    11941 tgccccccga cagtccagga gcttataccg cagcgctagc agctcggtca atgaaggtcc
    12001 ccggcaattc tcagcccggg caatactgag aagctttgaa ttgtccttca cttgcgaggg
    12061 ggcgatcgag tagatcccat cgcagtctcg aacgtcgatc gatgtgtggt catctgccga
    12121 aacaagggga acttgaagat tggggctcgg attgagcgat cccagcgacg aagcgctatt
    12181 ggccgcatat cgaagcgggg agtcccgggc attcgagata ttgctcggac ggtaccgcgg
    12241 gcccctgttc aacttccttc tgcgctcgtc gcgagatcgc gggcgggccg aggagctgta
    12301 ccaggacgtc tggatgaagg tcatcgagcg ctgcgacgat ttccgtggag acgccaagtt
    12361 ctcgacctgg ctgtatgcaa tcgcccgcaa tctcgcgatc gaccaccggc gaaagatgaa
    12421 gttccggggt cacgcccctc tcgatgcgac tcagccccgg accggacagt cgatcgggga
    12481 gcggctgggc aaccctggcc cctccaccga gcagctggcg atcggagggc tcattcgcgc
    12541 tcgtattggg gacgctgtgg aggagcttcc cgaggaacag cgggaggtgt tcctcttgcg
    12601 gcagctccaa gggctggcgt tcaacgagat cgccgaggtg gtcggcgttc ctgccaacac
    12661 cgtgaagagt cgaatgcgat acgccctgga acgcctgcaa ggcctcttga gcgatctgga
    12721 agagtccgat gaactgtgac gagtgcaaag agcagatctt cgagctgatc gagcaggaga
    12781 cgatcgatcc cgatggggtg cgcgccctgc tcgcccgctg tccggactgc cattcgctct
    12841 tcgacgagac caaggcggcc ttgcggacgg ccgcaatgct tcccctggag gagccgccgc
    12901 tctcgatcga tgccgccatc gttcgcgcgg cggccgagcg cgtgcccacg cagcgtcctt
    12961 ccggatggcg gcgcttgcaa gcggcccctt gggcgatggc agccgtcgcg ctgctcgcgc
    13021 tgggaatcgg ggtttggacc cttccgcgtg ggggtgagct ggagggaagc gacggtttcg
    13081 tcggaaagcg tgaggcccac ggcgagctga tcgccaggga cgagcttggg gatgcttcga
    13141 gccaggagcc ggtgatcgcc gcgctggaac ctgcgcccgc tcgggcgata gggggagctg
    13201 aggccgatgc acccgctacg ggtctcgaga cggcgagcag ccgttcagcg cgagccaagc
    13261 agagggcggg cgccccagtc gctccggcgg tcgctcggca ggtggccgga gcccggaagg
    13321 cttcgcccga ggcggctgca ccgcgattgg ctgcgcaaga acgcaaggag gatgcgatcg
    13381 ccgcgtgcca gcgacgagtc gcagaacacg aaggcgcaga gcaggacgag gcagacgcca
    13441 cgatcgatgc cgaacaggcg ctcgctcttg gcgagtgtta ccagaagctc ggcaagaaga
    13501 cgaaggcgcg acgatggttg acgcgggccg ctggggagcc cgtgacaaaa ctgcgcgctg
    13561 agcaagcgct acgtgggctg gacgccgagt agccccgtca gcgccggagt cgctcgatca
    13621 gcccgcgcca attgagcttg cggtctttgg cggttcgctg agacgttgct cgcgccgagg
    13681 cgagcgcatc gccgaggttc ggcgcgatct gcaaggcgtc gagctcgatg cccgcatcgc
    13741 gcgcggctgc tctccatcgc gaatcgatgc cgctgaagat gcagacgccg ccgagcattc
    13801 tcgcgatttc gtcagcggcg agaatcgccg cgacgcgttc tccgttcggc tcgccgagct
    13861 ggctgaggtc tacgatcgca atctcgatgc cggtgtccag catcgcgcgt ccgagcgcgt
    13921 cgacgcagtc gccgaagacc gccggttcgt gcacacccgt caggatcagc gcgaacgcgg
    13981 attcgtcgat gcgaagcggt ttcaggtgcc tggcggctcg cgcctccgat gcctcagtca
    14041 ctcgctcctc gcgcccgagc acgaagcctt ccaccgccgc ctcgactgca cgctgcgaga
    14101 agctcgtggg caagggattc gcttcgccct cggtcgacgc gaggatgagc cgcacgacct
    14161 cgatggcggt tgagggcgtg agatcgagca gagcgaggcg acggccgagc aggctcagta
    14221 ccgtgaagca ctcccgaaac tgaaaggagg catagtcgcc gcccgcgagt acgtccgccg
    14281 gcaattccat caagagcggg atcagcggcg ccgcttccgt gagcagatcc gctcgtgcca
    14341 ctctgccggt cggtggctta gctcgctcga tcaaagctcg gagtctagcg gcggcgcgct
    14401 ccacgtcgtt cacaatcgag ccaatctatc actctttggg gtgcccatgg accgccgtga
    14461 tataacgcga gcttagatgg ctgccactat cctcgtcgtc gatgacgaaa agaacatccg
    14521 gcgcacattg cgcatggtgc tcgaaggttc ggggttcaat acgctcgaag cggagtcggc
    14581 cgaggagtgc ctcgagacgc ttggacgaga cgaggtcgac ctggtgatcc tcgacgtacg
    14641 cctgccgaag atgagcggca tcgaggcgct acagaagatt cgcaacgagc ccgagacacg
    14701 gaagctccca gtcttgatgg tctcaggcca cgcgtccctg gccgaagcgg tgcaagcggt
    14761 ccagctaggc gcgacggact tcttcgagaa gccgctcgac cgggatcgaa ttctcctcac
    14821 ggtgcgcaac gccttgaaga cctggcagct tcagcgcgag gtcgagcaac tcagggccga
    14881 cgtcgaacat cgatacgaga tgatcgggga gagcgaggag atggcgaatc ttttcgcgca
    14941 gctcgaaaag gtcgcgccaa ccaacgggcg tgtgctgatt tcgggagaga gcggtaccgg
    15001 caaagagctg atcgcccggg cgattcaccg gctcagtcca cgcgccgaca agccgttcat
    15061 caaagtgaac tgcgcggcga tcccatccga gctgatcgaa agcgagctct tcggttacga
    15121 gcgaggcgcg ttcacaggcg cccagggccg gaagaagggc atgttcgagc tggcgaatgg
    15181 cggcacaatt tttctcgacg agattggcga catgagcgca agtgcgcagg ccaaggtcct
    15241 ccgtgcgctc cagagcggag agatctcgcg ggttggtggc gagaaaacga ttgcggtgga
    15301 cgtgcgcgtt ctcgcggcga ccaacaagga cctcgagctc gaagtgagag acggacgttt
    15361 tcgggaagac ctctacttta ggctcaacgt ggttcccatc gtgagccctc cgctccggga
    15421 tcgcaggcag gacatcccgc tgctggcaaa ggggttcctg cgcgaattct ccatcgaaaa
    15481 cggcctgcgt gagaagccga tcgatgaagg agtgctcgag gccttggccg agcgaccctg
    15541 gccaggcaac gtgcgcgagc ttcgcaacgt ggtcgagcgc atggcgatcc tgagtggaga
    15601 ccgaatcacg cttgacgatc tccccgagga gggcgtgctc agcgaagtcc ggagagaatc
    15661 ggccgcacct tcggacctgc cgtcgaggcg cgacgaaacg ggagaacgcc tgacattgcg
    15721 ggactatcgg actcgagccg agaaaaatta tatcatggag acgctcgatg aggtcggatg
    15781 gaacatctct cgggccgcta ccattctcgg tgtcgagcga acgaacctcc acaagaaaat
    15841 gcggagctat ggtatccgtc gagacgaacc atgatccgtc gatcactgag gctgatgtgg
    15901 acgggagccg ttctcggctt tctggcctac gttctcttct tcgtgcatct cggcgaacga
    15961 accaccttcg agcacctgat gcgagtcatc aacaccgaag aggcgaagga gctcggccgc
    16021 gatttgggcg ccgcgacagc ccgcctaagc aaacagatcg gcgaccaagt gcacgacgcg
    16081 acgaagccac cggctcagga tgcgggcaca tcccaggttc cggaggtggt ttcggacaag
    16141 cttcaaggca tggttgggca ctcgtatgag cgcgacgagc gcatggagac cgcgcttcag
    16201 aatgacgccc aataaccgtg gctagggcca ctcgagctgc agccgtttca tcttgcgcca
    16261 gagcgtagtc gggctgagcc cgagcagctc tgccgcgcgc tccttgttgc cggcggaatg
    16321 ttgtagcgcc cgaacgatgg cttcccgctc gacatcctga acgatgtccg cgagtgattg
    16381 gcgcccagga tgggagacgc gcccatttcc gttgccattc gagttgggga ggatgtcgga
    16441 aagttggacc ttgccgtcct gcgcaagagc cacgccctgt tcgatcaagt tctccaactc
    16501 gcggatgttt cccgggaaat cgtagccacg aaggtgatcc atgaccccgg gccccagcac
    16561 cgccggtctt cccatcttct tcccgtattt ctggaggaaa ctctcggtga tgggcgcgat
    16621 gtcctccttc cgttcccgca agggcggcag ctcgaaccgc gcgacgttga gacggtagta
    16681 gaggtcttcc cggaagcgct tttcttcgat gaggttccgt aggttctggt tcgtggcggc
    16741 gatgatccgt gcatcgacat tgatcggctt gttgtcgccg agtctccgga tttcgccttc
    16801 ttgaatcgcc cgcagaagct tggcttgaag ggacggcggc gtctctgcga tttcgtcgaa
    16861 aaagaaagtt ccgccgttgg cctcttcgaa aaggcctttg cgtgtttgca ctgcgccggt
    16921 gaacgctccg cgcatgtgtc cgaaaagctc gctctccagg agcgtttccg tgagggctgc
    16981 gcagttgatc gaaacgaagg ggctctccgc acgcaaggag tttgcgtgga tcgcgcgtgc
    17041 gaccagttcc ttgcccgtcc cgctttcgcc agtgatcaaa acggtcgcat cggtcggtgc
    17101 gatccgcacg atgcgaccca ggatatcgcg aatctcggca gactgaccga tgatgttgcc
    17161 gaagttgtac cgctcgcgaa agtcgtcacc gacggatcgc cgctcgcctg cgagctgacg
    17221 tgtgcgtgcg gctcgggcga ccttgatcag cagctcttct tcgacgaagg gcttctgaac
    17281 gaagtcgcgc gcgccgaagc gcatcgcctc gacggcgtct tcgattgtcc cgaatgccgt
    17341 catgacgatg acctccgtca gcggcgcgga ctcacgagtg cgctgcaaca cttcgaggcc
    17401 gcctacgtcg cccatcctca ggtccgtgat caccaagtcg tagccgctct cggccaccat
    17461 gtgacacgcg gactcgccgt tgggcgcctg gtctacggca tagccttccg cctgcaagat
    17521 ctccgagagc atcctgcgcg cactgtcctg atcgtcgacg atgagaatcc tggccatccc
    17581 ccaaccggtt ccgtctagcc ttgaccttac agcaaattgc tcgttcgtgc ccggtgcatc
    17641 aatcgagcac gtcgtgcatc gccgcaagca cctggctccg gaactccgac ggaaaaatga
    17701 ggcgtgggcg ctcgatgcgt ttgtttcgga gttggcccag gatgaaggag gcttccccga
    17761 gacgctgcaa gggttgatgt cgcagcgtga cccagagacc atccgtgctg ccgtccgtcg
    17821 gctcggggta tgggacgtcc tccgcaatgt ccagccagac ggtgaggtcg gtgcggaggc
    17881 cgccggctgc gacgatttcg cgggcgcgcg cgagggcctc atcccactga tcgcgtcggt
    17941 tctcgcgcag cggcacagtc tttggcagcg cgcggcagcg gagccgtttg ctgagctcgg
    18001 ccagggtcgg atcgcctccg tgctcccagg cgctcagaca gctgagaagc tcggcgtcgt
    18061 ccattcggag gtactcccca agcgagaggg attcgccgag tgcagccgct cggaacgcag
    18121 gaagcagcgg ggtcggcggt gttcctgcga atatcaactc gctgacgcgc atgaagatgg
    18181 cgcggaccaa ggcttcagcc gctctggtag ctttgtggtg atagacctgc tggtacatga
    18241 agtggcgtcc aagaaagaag ctctcggctg gtggcagacc cttgcggcct tgaatcgcta
    18301 gaacccatcg cccgtcgacg catgcaaacg tcaacgcttg aagcaaccag tcgaggtcgt
    18361 agagaccgta gcgaactccg gtcatgtgac tgtcgcgcag gaggtagtcg gcgcggtcca
    18421 cgtcgagtgt tccgctgacg ctctggctga gatagccgag ccggtagttg ccggctaaca
    18481 agctggtcac tcggtccgac atcccttcgg atatcgactc caaagcctga tgaacctcgg
    18541 tcgctggatc gagcaagatc tcttgcgtcc agagctcgtg atgtttggcg tgaggcagga
    18601 cctcttcgaa gaggtgagag aacggcccgt gtccgaggtc atgcaagagc gccgcggcga
    18661 gggcctccga tctggcttcc tcgtccaaac gctggtccga cggcagctcg tcgctgcagg
    18721 catcgatgcg gtgcaagagg gattgcatca cgtgcgctgt tccgattgca tgagaaaacc
    18781 gcgtgtgttc cgcgcctgga aagaccagcg aagcaaggcc gagctgccgc acgcgccgca
    18841 gacgctgaac ctcgcgtgta tcgaggaggg atcgaatgac ccgttccgcc atgccttcga
    18901 aggcgatcaa accgtgaacc gggtccctga ggatcacgct gcacggtacc actgcggatt
    18961 cagaacgcca gcgttccgaa gcggccgtct ctaggaacct cgaacgtcga gccctgccta
    19021 accacggcga ccgtgtcagg ctgccagagg ggcaaaaccg ggacgtctcg ggctaggatg
    19081 cgctgaacct cgtcgtaagc agcccgacgt cgatctcggt cgatctcggt tcggcccgtt
    19141 tcgaagagac catcgagttc ttcgtttgcg taccgccaac gattagcgcc ctcggcgtcg
    19201 ggaccgggaa tgcgtgatga agcgaaaaac cagctcagca cgtgaggctc gatcacttcg
    19261 ggcacctgga gcaaggtgat ctcgaattgt ccgcggttga ggtccgagat cagcgttgcg
    19321 gcttcggacg ggcgaacgtc gacctcgatg cccacgtccc gcaacatcgc agcgatcgct
    19381 cgagcgaccg attgacgaaa gcgctcggag gtggtgcgaa gcgccaagcg catgcgcggc
    19441 tccgaaccag gagggtccga gaagcccgca gcatcgagga gcgcgcgggc cttttcagga
    19501 tcgaacgctc gaggaggcgt cgcccgcgcc tccgcccagt gaccgtgtgg gatccagcta
    19561 tcggtgacgc gcgcgagccc ttcgtacttg gattgcacga gcagctctcg gtcgatcgca
    19621 tatccgatcg cttggcggac gcgaacgtcg ctcagcttcg tcgaacgagt ttggaagccg
    19681 agatacatcg tcgagatgcc ggcggcgctt cggatttcga agcgcgggtc gtcctgaaac
    19741 aagggaagca ggagcggagg aatcgaagcg atcgcgatgt cgccctcccc cgccagcatt
    19801 ctgagcgccc gggtgttgtc gtccctaatc acgaccagtc ggaggctcgg atatttcgtc
    19861 ttgccgcggt gccagtgtgg gttcgcccgc agctctaaga cgcctgcctg tcgcttgacc
    19921 aggcgatacg gtccggcccc gatgggaagc gggtcttccg gcgttgcgat ccggcggaat
    19981 gcatcttcgc tccgcacgat gggaagctcg agatcggtga tgaaggtcgc gtggggctca
    20041 tgcaggacga agacgatggt tcgcgagtcc agaacacgca tctcggcgat acgccgatag
    20101 gaatgggcgt agcggcttcg gagcgccgga tcgacgactc cctcgaacgt tgcgaccacg
    20161 tcctcggcat cgagcgcgct gccatcgctg aacgtcaagc catcacgaag gcgcacctcg
    20221 aactccgtcg cgctcatggc tttcaccgat tcggcgagat cggggatgac ttcgagcgtc
    20281 ctcggatcga tcgtgacgag cgaggcgaac aggagtcgcg tgacgcgaag cccgtagggg
    20341 tcgctgacga acctcgggtc gagctgttcg gcatcccgcg gcaacatgac gaccaaggcg
    20401 tcggcctcgt ctgcgacagg tcggttgcag ccgaacccaa tcactaggat gagcgcgagc
    20461 aggctgctag gctcccgccg atgcggtacc gggggcttat tctcatcgcg atcatctgcc
    20521 tgctcgtcgc cagcccgggg gcggatgcca aggagccggc gccccttgcg cagacgatcg
    20581 caagcctggt gcgtcaagcc ggtctcggag atgggctggg aattcatgtc gtccgcttgc
    20641 aagattcgga agaactgtac cgcaatcaac ccgaacgccc tcggaatcct gcgtccaacc
    20701 agaagctcct gacctcggca gcggcgctct ggcggctcgg ccccgccttt cgcgcaacga
    20761 cgaaggtcga gggaatcatc gaaaatgggc gcgtcgcgac gatggtgatt cgaccatcgg
    20821 gggatccgag cctcggctat gcaggcctcg acgcattggc ggagtcgctt cggctgcgag
    20881 gggtcgacac ggtggatcgc atcctgatcg acgacagcta cttcgaccag caaatccttc
    20941 cgcctgcttt cgagcagcaa ccgcacgaag cggcctcgtt tcgcgccgcg atctccgcct
    21001 tctccgtgga gcgtaacagc tacgtcgtgc acattgggcc gggcccgaaa gtcgacgcac
    21061 ctgggcgc
//
LOCUS       DASRZF010000123         7926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3178556, whole genome shotgun sequence.
ACCESSION   DASRZF010000123 DASRZF010000000
VERSION     DASRZF010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 7926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7926
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3178556"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VFG22_05730"
     CDS             complement(<1..196)
                     /locus_tag="VFG22_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415775.1"
                     /translation="MKKPVKVTLIATASLLAALLVALILVPVLFKDRIIERLRVELNE
                     RIDATVTFSDIDVSLLSTFPT"
     gene            296..595
                     /locus_tag="VFG22_05735"
     CDS             296..595
                     /locus_tag="VFG22_05735"
                     /inference="COORDINATES: protein motif:HMM:NF025715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4404 family protein"
                     /protein_id="HET6415776.1"
                     /translation="MESGTIQGMPRQQLNQALGALHEALESDEQIGPTDREALLEAVE
                     EIREALERADTGDARSQEAPLSGRVSTMIGDFEASHPRFAEILRSLSESLANLGI"
     gene            606..971
                     /locus_tag="VFG22_05740"
     CDS             606..971
                     /locus_tag="VFG22_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415777.1"
                     /translation="MTLTRRDFGRNVLAGSTGAPDAGSGGSGGDGGNACTLYPEQTAG
                     PFYLDLDLLRQDISEGKPGGLDTSGETFLRGTQVTDADGIAQCESVYPGLVPGPNDAH
                     SLSRTHLEQDGDHFAALLS"
     gene            complement(1214..2941)
                     /locus_tag="VFG22_05745"
     CDS             complement(1214..2941)
                     /locus_tag="VFG22_05745"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET6415778.1"
                     /translation="MSLAPKSMEPRFPLSIVNLLGDPMRKRILPIDSKRAMKLAERLT
                     GLHDYDDPDGSMREHLDATIESVLGVDWNTLGRFGVRYILNWHLSNRLRVVELLKQQP
                     EIGDMAIERPLVITGLFRTGTTYLHNVLAADPGSRVGRMWELAHPVGRPRDLFGDEKW
                     RRWRGSHEVAMDDVMVPEQEEAHKVTVDAWEEDFFLLENDLVVMKLAERLTGLHDYDD
                     PDGSMREHLDATIESVLGVDWNTVGRFGVRYILNWHLSNRLRIVELLKQQPEIGDISI
                     ERPLVITGLFRTGTTYLHNVLAADPGSRVGRMWELAHPVGRPRDLLGDEKWRRWRGSH
                     EVAMDDVMIPEQEEAHKVTVDAWEEDFFLIENDLVVMKLFVGLGAYDYGRRTLDWDMT
                     GSYRWHKRQLQILWAQRSARRWLLKCPWHLWNLEALLAVYPDARVIQTHRGLVDAIGS
                     QCSLSARIAAKFQRGFDLHQVGRFWLEYSQQGIERGLAARAALPASQIYDVRLADLKH
                     RPLETIESIYEHFELPFDETLASRFQDRIAEDPTAQLGEHAYDIADYGLSRDQIEGAF
                     VEYREAFGV"
     gene            3006..3542
                     /locus_tag="VFG22_05750"
     CDS             3006..3542
                     /locus_tag="VFG22_05750"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6415779.1"
                     /translation="MSRASNAPSAWVELHARRVRSAGTVLDLACGAGRHTRYLRTQGF
                     RVVAVDVDVSGIDDLIGDALVEVIEADLEMGPWPLGHRAFDGIVVTNYLHRPLLPKLV
                     DALAPEGVLIYETFAVGNEKYGRPSNPNFLLEHGELLETFGGHLEVLDYGHGLDEDPG
                     PAFRQRICAKKSAATEGA"
     gene            complement(3550..4341)
                     /locus_tag="VFG22_05755"
     CDS             complement(3550..4341)
                     /locus_tag="VFG22_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415780.1"
                     /translation="MKPIARMVYVPGREEVASRLGGEWSSLIEMAPASLDLMSPRGIA
                     TQGLQLISNAANPRRFDRLVADVQGRLRAASVPVLIEEHRNPAPLQEASSLTRPRRRW
                     LGQLALELYFAQLFRGYDAALDLRPSRFGVDGSGDAVWRPRPIYVRWNQRFLKGIRDV
                     YEGFFFDRAEQLDAGLAALGLGSSAGLLLGHLGDGNQRSVRFGSEQLRSLLREMADRR
                     QPEEPKLHPNFIAFGLLLTGLHELLEELDLAFDVRSAFMRTHPGT"
     gene            complement(4338..4517)
                     /locus_tag="VFG22_05760"
     CDS             complement(4338..4517)
                     /locus_tag="VFG22_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415781.1"
                     /translation="MTDLDLLIAGAMVTFLSVAGAYIAIRHRANETPVDSYTQAGTQS
                     PRELKPTAEPAEALR"
     gene            4874..5623
                     /locus_tag="VFG22_05765"
     CDS             4874..5623
                     /locus_tag="VFG22_05765"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6415782.1"
                     /translation="MLRSFLVQGASRGIGLEFVRQLLLRDEESQVFASCRSPERATGL
                     RELASSEPARLRLLRLDVTNEGTIETAAAEVAEQTGRLHLVLNVAGVLHGSDFGPEKK
                     LTQVRPEALREVFEVNAFGPLLVAKHFHRFLRHDERSVFASVSARVGSISDNRLGGWY
                     AYRGSKAAQNMFTKTLSIELARLAPMAVVVGLHPGTVDTELSKPFQRTVPQGQLQSTE
                     SSVRSMLSVLDGLAREDTGRVFDFRANEIMP"
     gene            complement(5625..6659)
                     /locus_tag="VFG22_05770"
     CDS             complement(5625..6659)
                     /locus_tag="VFG22_05770"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HET6415783.1"
                     /translation="MFGLGLRMAEAGFVPDPLVRAVTRQLLRARLRESVLGDSDARAR
                     EESRLIARLSEGPIAISVDAANAQHYQVPPAFFERVLGPRLKYSSGFWDSNARNLADA
                     EEAMLTLTASRAGIEDGMRVLDLGCGWGSFALWAAEHYPNAEILAVSNSSSQRTHILS
                     VAAERGLGNLEVETSDINDFEPGRAFDRIVSIEMMEHVRNHRLLFHRLASWLAPGGSA
                     FAHIFCHRSRAYTYENDDPSDWMARTFFTGGMMPSETLFHHYQDDLVLRDQWRVSGVH
                     YHRTCEAWLRRLDCNRDEVLPILRDVYGSEARLWFHRWRLFFIACSELFAYDAGAEWF
                     VAHYRWERRV"
     gene            6747..7754
                     /locus_tag="VFG22_05775"
     CDS             6747..7754
                     /locus_tag="VFG22_05775"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET6415784.1"
                     /translation="MDLDSRTIAITGATGFLGRHVAPRLLQEPDLRLRCLIRASSDAQ
                     ELENLGERVSFARGDVSEPDSLGPLFDGVWGVVNLAGYRDFWSRSRDLYYALNTRGAE
                     NVFRAALAAGVEKVVQVSTPLAFGVPEAIPFNEESEAGPHPSDYARSKHLGDEIGWRL
                     HREQGLPLTVVHLAAVIGAGDPRPTMEVRRAVEGWLPALVGADTTYTYVHVNDAAEAI
                     VRALLNPTSVGRRYLIGKERASTREYFDLIGELADVRVPKLNLPESLLIPVARVLEML
                     GALTGRRPPIPVDILKTTAAGSLIFDGTRAERELGLQYTPLRVALGEAIAEIREHAIC
                     P"
     gene            complement(7741..>7926)
                     /locus_tag="VFG22_05780"
     CDS             complement(7741..>7926)
                     /locus_tag="VFG22_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415785.1"
                     /translation="GTRGVIPLLFRMGVEIKASEHVSPFFLFELGPGISVGNGTADVS
                     FALRLWAGTSFWGVMGK"
BASE COUNT         1568 a         2516 c         2422 g         1420 t
ORIGIN      
        1 gggttggaaa cgtcgagagc agcgagacat cgatgtctga aaaagtcacc gtcgcgtcga
       61 tccgctcgtt gagctcgacg cgaagtcgct cgatgatgcg gtctttgaac aagaccggaa
      121 ccaggatgag cgcgaccaac agtgccgcca acagtgaagc cgtggcgatc aacgtaacct
      181 tgactggctt cttcattctc ccctttgtgg cggtttctgt agcacggcgg cgccctttgc
      241 tcttcccgca aaggtttagc agcccgaagc ctgcgttttc tcctttcctt gggggatgga
      301 gagcggcaca attcaaggca tgccccgtca gcagttgaat caggcgctgg gcgccctcca
      361 cgaggcgctc gagtccgacg aacagatcgg accaaccgac cgcgaagcac tgctcgaggc
      421 ggtcgaggaa atccgcgaag cactggaacg tgctgacacc ggagacgcgc ggagccagga
      481 ggcgccattg agcgggcggg tgtccaccat gatcggagac ttcgaagcat cgcacccgcg
      541 ctttgccgag atccttcgga gcctgtccga gagtctcgcc aacctcggta tctaggacag
      601 ccgaaatgac gcttacgaga cgagactttg ggcgaaacgt attggccggc agcactggcg
      661 ctcccgatgc cggctcagga ggcagcggcg gcgacggcgg caacgcatgc acactctacc
      721 cggagcaaac ggccgggccc ttctatctgg acctcgacct gctgcgccaa gacatcagcg
      781 aaggcaagcc cggcggcctc gacacgagcg gcgagacctt tctccgcggc acccaagtga
      841 ccgacgcgga cggcatcgcg cagtgcgaga gcgtctaccc cgggttggta cccgggccga
      901 acgacgcaca ttcactttcg cgtacgcacc tcgagcagga cggagaccac ttcgcagctc
      961 tactttcctg agcccgtgac cgaagagatc tacctcagct cgccgtacga tgcccgtggt
     1021 cagaaagaca ccccgaacgc gctcgacggc atcgcaaccg ccaaccctgc cccgctcgca
     1081 atggtcacga gcaatacttc gacaggctac gtcgcgagca aggttggaac ggcacgcgcg
     1141 cagtgtcctc gagtgttcag catgagcttg gtccttacct accacaatcc attggctcga
     1201 caccatggga ctcttagacg ccgaatgcct cgcggtactc gacgaacgcg ccttcgatct
     1261 gatcccgact gagcccgtag tccgcaatgt cgtaggcgtg ctctccgagc tgcgcggtgg
     1321 ggtcctccgc gatgcgatct tggaaccgcg aggcgagcgt ttcgtcgaag ggaagctcga
     1381 agtgctcgta aatactctcg atggtttcga gggggcgatg cttgaggtcg gcgaggcgga
     1441 catcgtagat ctgggatgca ggcagcgccg ccctggctgc cagcccccgc tcgattccct
     1501 gctgtgagta ctccagccag aatcggccga cctggtgcaa gtcgaaacct cgttggaact
     1561 tggcggcgat gcgggcactc aagctgcact gcgaaccgat cgcatcgacg aggccgcggt
     1621 gggtctgaat gactctggca tcgggataca cagcgagcag cgcttctagg ttccataggt
     1681 gccaagggca tttgagtagc cagcggcgtg cagaacgctg cgcccagagg atttgaagct
     1741 gccgcttgtg ccatcggtac gaccccgtca tgtcccagtc gagcgtgcgg cgcccgtagt
     1801 cgtaggcacc caatccaacg aacagcttca tcaccaccag atcgttctcg atgaggaaga
     1861 agtcctcttc ccatgcatcg acggtgacct tgtgcgcctc ttcctgttcg gggatcatca
     1921 cgtcgtccat ggcgacctcg tggctaccgc gccaacgcct ccatttctca tcgccaagca
     1981 agtcacgcgg gcgcccaact ggatgcgcga gctcccacat tcgacccacg cggctaccgg
     2041 gatcggcggc cagaacgttg tgcaagtagg tagtacccgt tcgaaacagc cccgtgatga
     2101 cgagcggacg ttcgatggag atgtcgccta tctcaggctg ctgcttcagc agctcgacga
     2161 tgcgcagccg attgctgaga tgccagttca agatgtaccg gaccccgaag cgaccgaccg
     2221 tattccaatc cacaccgaga accgactcga tcgtggcgtc gaggtgttcg cgcatcgaac
     2281 catcggggtc gtcatagtcg tgtaggcccg tgagccgttc ggcgagcttc atcaccacca
     2341 gatcgttctc gaggaggaag aagtcctctt cccatgcatc gacggtgacc ttgtgcgcct
     2401 cttcctgctc ggggaccatc acgtcgtcca tggcgacctc gtgactaccg cgccaacgcc
     2461 tccatttctc atcgccaaac aagtcacgcg ggcgcccaac cggatgcgcg agctcccaca
     2521 ttcgacccac gcggctaccg ggatcggcgg ccagaacgtt gtgcaggtag gtggtacccg
     2581 ttcgaaacag ccccgtgatg acgagcggac gttcgatggc catgtcgcct atctcgggct
     2641 gctgcttcag cagctcgacg acgcgcagcc gattgctgag atgccagttc aagatgtacc
     2701 ggaccccgaa gcgaccgagc gtattccaat ctacaccgag aaccgactcg atcgtggcgt
     2761 cgaggtgttc acgcatcgaa ccatcggggt cgtcatagtc gtgtagacct gtgagccgct
     2821 cggcgagctt catcgctcgt ttggagtcga tcgggaggat gcgtttccgc atcggatcgc
     2881 cgaggaggtt cacgatggaa agcgggaacc tcggctccat cgattttggc gctaggctca
     2941 tgcttggagc cgcaccctat caaaccgtgg cttctggaca atttcgtgtc agaaagcacc
     3001 aagggatgag ccgagcgagc aatgcaccct ccgcctgggt cgagcttcac gcgcgacgcg
     3061 tccgcagcgc cgggacggtc ctcgatctgg cctgcggcgc tgggcggcat acccgatatt
     3121 tgcgcacgca gggctttcgc gtcgtcgccg tggatgtcga tgtttccggc atcgacgacc
     3181 taatcggcga tgcgttggtc gaggtcatcg aagcggacct ggagatggga ccctggccgc
     3241 ttgggcaccg agcgttcgat ggaatcgtag tcacgaacta tctgcaccgc cctctcttgc
     3301 caaagctcgt ggacgctctc gcgcccgagg gcgtactgat ctacgaaacc ttcgcggtgg
     3361 gcaacgagaa gtacgggcgc ccgagcaatc cgaacttcct cttggagcat ggggagctgc
     3421 tagagacatt cggagggcac ctcgaggttc tcgattatgg gcacggactc gacgaggacc
     3481 cgggtcccgc gttccgccag cgcatctgcg cgaagaaatc tgcagcgacc gagggcgcct
     3541 gagccggggt cacgtccccg gatgcgtgcg catgaacgcg cttcgcacat cgaacgcgag
     3601 atcgagctcc tcgagtagct catgaagacc ggtgagaagg aggccgaaag cgatgaagtt
     3661 cgggtggagc ttgggctctt cgggctggcg tcggtccgcc atctcgcgaa gtagggatcg
     3721 caattgctcc gagccgaacc tcacgcttct ctggttaccg tcgcccaaat gccccagaag
     3781 cagaccggct gaagagccca agccaagcgc cgcgagaccc gcgtccaact gctctgcacg
     3841 atcgaagaag aatccctcgt aaacatcgcg gatccccttc agaaaccgtt ggttccagcg
     3901 aacgtaaatg ggacgcggcc tccagaccgc gtcgcccgag ccatcaacgc caaagcgtga
     3961 gggtctcaag tcgagggctg cgtcgtagcc tctgaacagc tgcgcgaagt acagctcgag
     4021 cgcgagctgt ccgagccaac gtcgccgtgg tcgagtgagg ctcgacgcct cctgcaaggg
     4081 cgccgggttt cggtgctcct cgatcagaac ggggaccgaa gccgcgcgaa gccgtccttg
     4141 tacgtcagca accaatctgt cgaagcggcg cgggttggcc gcgttggaga tcaactggag
     4201 gccctgcgtc gcgatgcccc ggggcgacat cagatcgagc gaagcgggag ccatttcgat
     4261 caagctgctc cactcgccgc ccagtctcga ggcaacctct tcacggcctg gcacgtagac
     4321 cattctcgcg atcggcttca tcggagtgcc tccgctggct ctgccgtggg cttgagctca
     4381 cgaggggatt gcgtgccagc ctgcgtgtac gagtccactg gcgtctcatt ggctcggtgc
     4441 cgtatcgcga tgtaagcgcc cgccacggac aggaacgtca ccatcgcgcc ggcgatgagg
     4501 aggtcgaggt cagtcattgc gcccaccggt gaggagcagg ccccccgcgg agaggattga
     4561 cggtacccag aggcccacga atagaccccg ctcacggtcg cccgagaacc agagccagat
     4621 cgacaggcaa aatgaaatgc cggctgcagc gaagaacagc gccttggatg cgctgatccc
     4681 agagaacctc ggacgggcgc ccagccggga cgttgataac gagggggtgg tcgaatgatt
     4741 catggtttac ctccaacggg cttctagctc ttgtctaggt attgtcaatc aaacatggac
     4801 aaaatataga caaccgtgct aggactcgct agacgcagga ggagacgaaa gatgcaaacg
     4861 atctcagatg ccaatgcttc gtagtttcct cgtccaaggc gcgagccgcg gaatcggcct
     4921 ggaattcgtg cgccaactcc ttctccgaga cgaggaatcc caggttttcg cgtcctgccg
     4981 gagtccggag cgagccaccg ggcttcgaga gctcgcttcg agcgagccgg cgcggctacg
     5041 gctcctacgt ctggacgtca cgaacgaggg cacgatcgaa acggcagccg ccgaggtggc
     5101 ggagcagacg ggtcgacttc acctcgtgct caacgtcgcc ggcgtgcttc acggttccga
     5161 cttcggccct gagaagaagc tcacccaggt gcggcccgaa gcgctgcgcg aggttttcga
     5221 ggtcaacgcg ttcggacctc ttctcgtggc caagcacttc catcggtttc ttcgacacga
     5281 cgagcgctcg gtctttgcca gcgtttcggc acgcgtcggg agcatctcgg acaaccggct
     5341 cggcggctgg tacgcctatc gcgggtccaa ggccgctcag aacatgttca ccaaaacact
     5401 ctcgatcgag ctagctaggc ttgcccccat ggcggtcgtc gtcggactgc atcccggcac
     5461 ggtcgacacc gagctttcca agccctttca gcggacggtg ccgcaagggc agctgcaatc
     5521 cacggaatct tcggtgcgtt ccatgctctc ggttctcgat ggcctggcgc gtgaggacac
     5581 gggtagggta ttcgacttcc gggcgaacga gatcatgccc tagctcagac gcgtctctcc
     5641 catcggtagt gcgcgacgaa ccattcggcg cccgcgtcgt aggcgaagag ctcggaacag
     5701 gcgatgaaga aaagccgcca gcgatgaaac caaagtcgcg cctcggaccc gtagacatcg
     5761 cgcagaatcg gaagcacctc gtctctgttg cagtcgaggc gccgcagcca tgcctcgcat
     5821 gtccggtggt agtgcactcc gctcacgcgc cactggtcgc gtagaaccag gtcgtcttgg
     5881 tagtggtgga agagagtctc ggagggcatc atacctccgg tgaaaaaggt tcgcgccatc
     5941 cagtcgctgg ggtcgtcgtt ttcgtacgtg taggctcgag accggtggca aaagatgtgt
     6001 gcgaaagccg agccgcccgg tgccagccag ctcgccaggc ggtgaaagag cagccgatgg
     6061 tttctcacgt gctccatcat ctcgatggac acgatacggt cgaaggcacg ccccggttcg
     6121 aagtcattga tgtcgctggt ctcgacctcg aggttcccga ggcccctctc tgcagcgact
     6181 gagagaatgt gagtccgttg tgagctcgag ttcgacacag caagaatttc cgcgttggga
     6241 tagtgctccg cggcccagag tgcgaaagag ccccacccgc accccaagtc gaggacgcgc
     6301 atcccgtcct caatccccgc gcgtgacgcc gtcagcgtca gcatcgcctc ctcggcatcc
     6361 gccaggttgc gagcattgga gtcccaaaac ccggagctat acttgagcct cggtcccaag
     6421 acgcgctcaa aaaatgcggg aggaacttgg tagtgctgag cgttggccgc atcgaccgaa
     6481 atcgcgatcg ggccttcgct cagccgggcg atgagtctcg attcctcgcg cgcgcgcgca
     6541 tcggagtctc ccaggacgct ctcgcgcagc cgcgcccgga gaagctgccg cgtgaccgcg
     6601 cgaactagag ggtcggggac gaaacctgct tccgccatcc tgagtccaag cccaaacatc
     6661 aaccttcctc atccacggga taaggcgcag ctcgatgcgc gcaagactgg ccttggcgat
     6721 ttctgggcta accctagaga cgcgggatgg acttggactc acgcaccatc gcaatcaccg
     6781 gtgcaaccgg attcctcggc cgccacgtag cgccccgtct tctgcaggaa ccggacttgc
     6841 gccttcgatg cctcattcgc gcttcgagtg atgcacagga gctcgagaac ctgggcgaac
     6901 gagtcagctt cgctcgaggc gacgtcagcg agccagattc gctaggtccc ctcttcgatg
     6961 gcgtttgggg cgtggtcaac ctcgcggggt accgggactt ctggagtcgg agccgtgacc
     7021 tctactatgc gctcaataca cgcggcgctg agaacgtctt tcgcgcagcg ctcgccgccg
     7081 gggtcgagaa agtggtccag gtcagcacgc cgttggcgtt cggcgtgccc gaagcgattc
     7141 cgttcaacga ggagagcgaa gcgggaccgc acccctccga ttacgcgcga agcaagcatc
     7201 tgggtgacga gatcggctgg cgcctccacc gcgaacaggg acttccgctc acggtggttc
     7261 accttgccgc cgtgatcggc gcaggagacc cgcgcccgac gatggaggtt cgccgcgcag
     7321 tcgagggttg gctccctgcg ctcgtcggcg ccgatacgac ctatacctat gtgcacgtca
     7381 acgacgctgc agaagccatc gttcgcgcac tccttaaccc gacgagcgtc gggcgccggt
     7441 atctcatcgg caaggagcgc gcatcgaccc gcgagtactt cgatctcatc ggtgagctcg
     7501 ccgacgtccg cgtgcccaaa ctgaacctcc cggaatccct gctcatcccg gtcgcccggg
     7561 tcctcgagat gcttggggca ttgacgggga gacgcccccc gattcccgtg gacatactca
     7621 aaaccacggc agccggatcg ctgattttcg acgggacgag agccgaacgt gagctcggtc
     7681 tacagtacac gccgctacgc gtcgcgctcg gcgaggcgat cgcggagatt cgcgaacacg
     7741 ctatttgccc atgacgcccc agaacgacgt gcccgcccat aatcgcaagg caaacgacac
     7801 atccgccgtc ccgtttccaa ccgagatacc gggccccaac tcgaagagga agaaaggcga
     7861 gacatgttcg gatgctttga tctccacccc catgcggaag agcagcggaa tcacaccccg
     7921 ggtccc
//
LOCUS       DASRZF010000124         2114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152 k141_83878,
            whole genome shotgun sequence.
ACCESSION   DASRZF010000124 DASRZF010000000
VERSION     DASRZF010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83878"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /locus_tag="VFG22_05785"
     CDS             complement(<1..1112)
                     /locus_tag="VFG22_05785"
                     /inference="COORDINATES: protein motif:HMM:NF024592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HET6415786.1"
                     /translation="MEPIFLHLGVALGLGLLVGVQRQRVDDPLAGIRTFPLITILGAL
                     LAELSLRLDATWIFAVGLGSLAAVLATGYAITGRTGRYKLGLTTEIAALYMYIVGAFI
                     AIGAVTVGVVMGGLCALLLHLKEPMEGFAARLTQADVKSVMQFAAIALVVLPVLPNRT
                     YGPYDVLNPFEIWLMVVLIVGISLAGYVLYKLLGARAGTLLGGVLGGLISSTAATASY
                     ASRAKQHPSLAPQVALLIMVASTVAYLRILVEIAVAAPSVLSDVAPPLLIISLANAVI
                     CGVALVFIRGDAGDLAKQDNPSELRVALVFGALYGLIIFAIAAARETYGDPGLYVIAT
                     ISGLTDVDAITLSTANLIEAGRLDPGSGWRAIVLASL"
     gene            1196..>2114
                     /locus_tag="VFG22_05790"
     CDS             1196..>2114
                     /locus_tag="VFG22_05790"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HET6415787.1"
                     /translation="MASKNTFTLSTVPRDLTAGVVVSLVALPLCLGIALASNAPLFSG
                     LVAGIVGGILVGAISGSQTSVSGPAAGLAAVVAAQIITLGSFEAFLAAVALAGVIQVG
                     LGLIQAGSLARFFPSSVIKGLLAAIGVILILKQIPHVVGHDADPEGDMSFLQPDHENT
                     FSELLRTITDLHQGAALIGLLSVGVLVLWSRVKPLRESLLPAPLVVVLLGVGMNFVFR
                     GLGDQWFVGGSHLVQVPVAEDLSSFLGFLSKPDFSLWANPALYSAALTVAIVASLETL
                     LNLEAVDNLDPKQRHSPPSRELIAQGVGNI"
BASE COUNT          392 a          665 c          663 g          394 t
ORIGIN      
        1 aggctagcga gcacgatggc gcgccaaccg gaccccggat cgaggcgacc cgcctcgatc
       61 aaattggcgg tggacagtgt gatcgcgtcg acgtcggtga ggcctgagat tgtcgcaatc
      121 acatagagac ctggatctcc ataggtttcc cgggccgctg caatggcgaa gatgatcaaa
      181 ccgtacagtg caccgaacac gagcgctacg cggagctccg aagggttgtc ttgcttcgcg
      241 aggtcgccgg cgtccccgcg gatgaagacg agcgcaacgc cgcagatgac tgcgtttgct
      301 agggagatga tgagcagcgg cggtgctacg tcggacagca cggacggcgc cgccaccgcg
      361 atctccacca agatgcgcag gtacgccacc gtggaagcga ccatgatcag aagcgcgacc
      421 tgaggcgcga gggacggatg ttgcttggcg cggctcgcgt agctggcagt tgctgcggtg
      481 ctcgagatca aacctccgag cacgccccca agtagcgtcc cagctcgagc gccgagcagc
      541 ttgtacagca cgtaacccgc caagctgatt ccgacgatca acacgaccat cagccaaatc
      601 tcgaacggat tgagcacgtc gtacggcccg taggtgcggt tcgggaggac cgggagcacc
      661 accagcgcga tcgccgcgaa ctgcatcacg gatttcacgt cggcctgggt cagtcgggca
      721 gcaaaaccct ccatcggctc tttcaagtgc agcagcaaag cgcagagccc gcccatcacg
      781 actcccacgg tgaccgcgcc gatcgcaatg aatgctccca cgatgtacat gtagagagct
      841 gcgatttccg tggtgagccc gagcttgtag cggcccgttc ggcccgtgat cgcgtaaccc
      901 gtggcgagaa ccgcagcgag gctcccaagc ccgaccgcaa aaatccacgt cgcatcgagc
      961 ctaagggaaa gctccgccag gagtgccccc aagatcgtaa tcagcgggaa ggtgcgaatc
     1021 cctgccaacg ggtcgtcgac gcgctggcgc tgcacaccga cgagcagccc gagtcccagg
     1081 gccaccccta ggtgcaggaa gatcggctcc attgcgagat gatggccgtt tggtcgttga
     1141 actgccagtc gacagcggtg ccgattgtgc gttatgcgag gactccggag aatcgatggc
     1201 gtcgaagaac actttcactt tgtctaccgt cccgcgtgac ctgaccgccg gggtggtcgt
     1261 ctcgctcgta gccctgcccc tttgcctcgg tatcgccttg gcctcgaacg cgcccttgtt
     1321 ctccggcctg gtggccggca tcgtaggcgg cattttggtc ggtgcgatca gcggctcgca
     1381 gaccagcgtg agtggtccgg ccgccgggct cgctgccgtc gtcgcggccc agatcataac
     1441 actcgggtcg ttcgaagctt tcctcgctgc agtcgctctg gcgggcgtca ttcaggtggg
     1501 cctcggtctc atacaggcgg gctcgctcgc gcgcttcttt ccatccagcg tgatcaaggg
     1561 cttgttggct gcgatcggtg tgatcctgat tctcaaacag attccgcacg tcgtggggca
     1621 tgacgccgac ccggaagggg atatgtcgtt cttgcagcca gaccacgaaa acacgttctc
     1681 ggaattgctt cgaacgatta cggacctcca ccagggcgcc gctctaatcg gcctcctttc
     1741 ggtgggggtg ctcgtgcttt ggtccagggt caagccgctc agggagtccc tcttaccggc
     1801 gccgctggtg gtggtcctgc tcggggttgg gatgaacttc gtctttcgcg ggcttggaga
     1861 tcagtggttc gtcggcggca gccacttggt gcaggtgccc gtcgccgagg acttgtcgag
     1921 cttcctcggg ttcctcagca aacccgattt ctcgctctgg gcaaacccag cgctctactc
     1981 agcggctttg acggtcgcaa tcgtggcgtc cctggaaaca ctgctcaacc tcgaagcggt
     2041 ggacaatctc gaccccaagc agcgccactc gccaccgagc cgcgagttga tcgcgcaagg
     2101 ggtcggaaac attg
//
LOCUS       DASRZF010000125         6825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2151117, whole genome shotgun sequence.
ACCESSION   DASRZF010000125 DASRZF010000000
VERSION     DASRZF010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 6825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6825
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2151117"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="VFG22_05795"
     CDS             <1..802
                     /locus_tag="VFG22_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415788.1"
                     /translation="TALPAKYQATLAKPPNVEWSLTAELGFLSVVSHIVQFGQDGSRF
                     NYRSEGGQDVLFPFFRLSTDLKFKGRHAIIFLYQPLKLVGETVLSEDTVFDDVLFPAG
                     TPLDSVYGFPFWRLSYAYDFLRRAGDEVSIGFGLQLRDATITFTSADGTLRADRRDLG
                     PVPLLKFRGRWLFENGVWWGTEIDGIYAPISGINGSDEKITGAFLDASLRVGYEFTKR
                     VSAYFNVRYLGGGAEGTQKDPTPPSDGFTKNWLHFLTFSLGVEFRAAP"
     gene            complement(821..2041)
                     /locus_tag="VFG22_05800"
     CDS             complement(821..2041)
                     /locus_tag="VFG22_05800"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF005890.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HET6415789.1"
                     /translation="MPEAVIVSTARTPIGRAFKGSLIEMRPDDLAAIAVKAALAKVPN
                     LDPASVEDLMIGCAQPAGEQGYNLARVVSLLAGLDSPGVTVNRYCSSSLQTIRMAAHA
                     IKAGEGDVFVAAGVECVSRFGLGKADGMENTKNPKFSAAEERMAELMTPDGGNWELGD
                     GIADVYITMLQTAENVAQHMAISREAQDEFAALSQQRAEAAQKSGHFSKEITPVTTPS
                     GTLVDTDDCPRHGTTAEKLAALKPVLLGQLGERATVTAGNACPLNDGAAAVIVMSDKR
                     AQQLGIKPLARIVSTGVSAINPEIMGLGPVDATKQALRRAGLTSKDIDLFEINEAFAA
                     QVIPSALELGIPLDKLNVNGGAIALGHPFGQTGARIMTTLINNLQSRGARYGVETMCV
                     GGGQGMAAVIERLD"
     gene            complement(2045..3718)
                     /locus_tag="VFG22_05805"
     CDS             complement(2045..3718)
                     /locus_tag="VFG22_05805"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HET6415790.1"
                     /translation="MSRLLAVEAKLLGPGAPFELAEEEFLGEKVRVFANRARSLRDVL
                     LRAREFSGKEYMVFRAAEQERRYTFGEHEKLVARVAAGLSERFGVGPGDRVAILAANC
                     PEWIISFWATVSLGAICVGLNGWWTEDEIRYALGHSTPKVLIADRKRAARITHELDVP
                     MLVVEEGFDELVSAHPEAELPTQPIAESDPAIILYTSGTTGRAKGVVHTHGNVTNMLM
                     VSFFHGARLMMANPRQSELPQLANSILVTSPLFHVSGLHCAAVTALAGGAKTVWPMGR
                     FDAEVTLELIEREKVTGWGYTATVLHRLLNHPSVGDYDLSSFRSVGGGGSPIPAPLIE
                     KARALFPQCSHTMGVGYGLTEGTAFATLNAGEELTDDPTSVGRPMPIVQVEIRDALGR
                     AVPEGEEGEIHLRGPLVMLEYWDDPEATDAAIGPGRWLNTGDVGRLRDGKLYIASRKR
                     DLILRGGENVYPFEIEQRLEAHPAIAEAAVLGVDHEELGQEVKAFVVFESDRMLEVEE
                     MRRWVAQALAYYKVPTQWETRKSPLPRNATGKVLKIALRDAQDAMFIEE"
     gene            complement(3715..4110)
                     /locus_tag="VFG22_05810"
     CDS             complement(3715..4110)
                     /locus_tag="VFG22_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415791.1"
                     /translation="MGRVIFDEKDKPLMRMTYQGLCDDAEWKRHVDVMSDWARRGDPY
                     AVVIDAREVGRVPPTQRREIMEWINRDRQHLKMNCAGGALIFSSALQQGLWTAISWVT
                     PSPIPVKIFRDLSSAEAWLHDQLQEKLAS"
     gene            complement(4149..4913)
                     /locus_tag="VFG22_05815"
     CDS             complement(4149..4913)
                     /locus_tag="VFG22_05815"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6415792.1"
                     /translation="MLDFPPGAGLIIGGSGGIGSAIARGFAAAGVTLAITYRHNEDAA
                     NAVADEIRSGGGTCSVHRVELGDLESVRACMDGLVAEHGQIHTVAHAAGTHIDQPYVS
                     QVTPEQWRNTMDWDVNGFFHVVHTALPHLRESKGSIVFVSSAGLKRFPPGDVLSVAPK
                     GAIEALMRGIAREEGRHGVRANNVAVGVVDAGMFPKLVERGELSQGWIDAAIRNTPLR
                     RFGKPEEIADAAVFLASTRARYITGQTLFVDGGYTL"
     gene            complement(4906..6471)
                     /locus_tag="VFG22_05820"
     CDS             complement(4906..6471)
                     /locus_tag="VFG22_05820"
                     /inference="COORDINATES: protein motif:HMM:NF005801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadD3 family acyl-CoA ligase"
                     /protein_id="HET6415793.1"
                     /translation="MAGTIPDMLAQKADALGKTKAIVDGDESLSFLDLAARVDEAARA
                     LIALGIESGDRVAIWAPNIWEWIVAALAIHTVGGVMVPVNTRYKGIEAAYLLEKSGAK
                     ALFTVTGFLDVDYVALLRNAPAEVPELAQIIVLRGEAPEGTLSYEAFLERAEEVSPSA
                     AKTRAATIRPDDIADILFTSGTTGRPKGAMCTHAQNLRVFDQWSSIVGLREGDRYLIV
                     LPFFHSFGYKAGWFSALLRGATIYPEPLFDVNVVLRRIEEDAITMLPGPPALYQSILL
                     HPDRAKFDISSLRLAVTGAAAIPVELIQRMKDELGFETVITAYGLTESCGVVTMCRLD
                     DDAETIATTSGRAIPDVEVRIVDNAGNSMPANEPGEIVVRGYNVMKGYFDAEEETREA
                     IDPDGWLHTGDIGTMDERGYVKITDRLKDMFIVGGFNAYPAEIENTLLQMPGVGEVAV
                     IGVPDERLGEVGMAFVVPAPGEVLAPEAVIAWSRENMANFKVPRRVEILGALPRNATG
                     KVLKFELRERAGA"
BASE COUNT         1222 a         2187 c         2053 g         1363 t
ORIGIN      
        1 gactgctctc ccagccaagt atcaggcgac gctggcaaag ccgcccaacg tagaatggtc
       61 gctcaccgcg gagctcggct tcctgtcggt cgtgagtcac atcgtccagt tcggccagga
      121 cgggagccgt ttcaactacc gttccgaagg aggacaagac gttctctttc ccttctttcg
      181 actgagcacc gatctgaagt tcaagggccg gcatgcgatc attttcttgt accagccgct
      241 caagctcgtt ggcgaaacgg tcttgagtga agacacggtc ttcgacgacg tgctctttcc
      301 cgcaggtaca ccgctcgatt cggtctatgg gtttcccttc tggcgcctca gctacgcata
      361 cgattttctt cgacgcgcgg gcgacgaggt ttcgatcggg tttgggctgc agctccgcga
      421 tgccacgatc acgttcacct cggccgatgg cacgttgcgc gctgaccggc gcgacctcgg
      481 acccgtgccg ctgctgaagt tccgcggccg atggctcttc gaaaacggtg tctggtgggg
      541 caccgagatc gacgggatct atgcgccgat cagcgggatc aacggaagcg acgagaagat
      601 caccggcgca tttctcgatg cgagccttcg cgtgggttac gagttcacga agcgcgtgtc
      661 tgcgtatttc aacgtgcgct acctgggcgg cggggccgaa ggcacccaga aggacccaac
      721 ccctccgagc gatggcttca ccaagaactg gctgcacttc ctgacgttca gcctgggcgt
      781 cgagtttcgc gccgcgcctt gatcgccgcg ccgacccttc tcagtcgaga cgttcgatta
      841 ccgccgccat gccctggccg ccgccgacgc acatggtttc gacaccgtac ctggcaccgc
      901 gcgactgaag gttgtttatg agtgtggtca tgatccgagc gccggtttga ccaaacgggt
      961 gaccgagtgc gatggcgccg ccattgacat tgagcttgtc gagggggatg cccagctcga
     1021 gcgcggaagg aatgacctgg gcggcaaagg cctcgttgat ttcgaacaag tcgatgtcct
     1081 tgctggtgag gccagcgcgc cgcagcgcct gtttcgtcgc atcgactgga ccgaggccca
     1141 tgatctcggg attgatcgcg gaaacgcccg tcgagacgat gcgcgccaga ggcttgatgc
     1201 caagctgctg cgcccgtttg tcactcatca cgatcacggc cgcagcgcca tcgtttagcg
     1261 gacacgcgtt gccggccgtc accgtagcgc gctcacccag ctgcccgagc aggaccggtt
     1321 tgagagctgc gagtttctct gccgtggtgc catgccgggg gcagtcgtcg gtatcgacca
     1381 gggtaccgct cggcgtcgtt acgggcgtga tttccttcga gaagtgacct gatttctgag
     1441 cggcttccgc gcgctgctgg gaaagggctg cgaactcgtc ttgtgcctcg cgggaaatcg
     1501 ccatgtgctg cgcaacgttc tcggccgtct ggagcatcgt gatgtacacg tcggcgatac
     1561 cgtcgccgag ctcccagttt cctccatccg gcgtcatcag ctcggccatc cgctcttcgg
     1621 cagcagagaa cttcgggttc ttggtgttct ccattccatc tgccttgccc aatccgaaac
     1681 ggctcacgca ttccacgcct gcagcaacga acacgtcgcc ttcgccggct ttgatggcgt
     1741 gcgccgccat tcgaatcgtc tgcaatgagg aggagcagta acggttgacg gtcacgcctg
     1801 gggagtcgag acccgccagc aggctcacga ctcgcgccaa gttgtagccc tgctctccgg
     1861 cgggttgcgc gcagccgatc atgagatctt cgacgctcgc cgggtcgagg tttgggactt
     1921 tcgcgagcgc tgctttcact gcgatcgccg ctaagtcatc ggggcgcatc tcgatgagcg
     1981 aacctttgaa ggcgcgtccg atcggggttc gcgcggtgga aacgatcacg gcttcgggca
     2041 tgacttactc ctcgatgaac atcgcatctt gcgcgtcgcg cagggcgatc tttagcacct
     2101 ttccagtcgc gttgcgggga aggggggatt ttcgggtctc ccactgtgtt ggcaccttgt
     2161 aataggcgag cgcctgagcc acccatcgtc gcatctcctc gacctcgagc atccggtcac
     2221 tttcgaagac cacgaatgcc ttcacttctt gaccgagctc ctcgtgatcg actcccagga
     2281 cggccgcctc cgcgatcgcc ggatgtgcct ccaggcgctg ctcgatctcg aatgggtaga
     2341 cgttctctcc accgcgaaga atgaggtcac gcttgcgcga agcgatgtag agcttgccgt
     2401 ctcgaagtct tccgacgtcg ccggtattga gccagcgacc aggcccgatt gcggcgtcgg
     2461 tcgcttcggg atcgtcccag tactcgagca tcaccagagg gccgcgaagg tgaatctcgc
     2521 cttcctcacc ttcgggcacg gcgcggccca gagcatctcg aatctcgact tggacgatcg
     2581 gcataggtcg ccctacggat gtggggtcgt cggtgagctc ttcaccggcg ttcagggtcg
     2641 cgaacgcagt cccttcggtg aggccatacc cgacgcccat cgtgtggctg cactgaggga
     2701 agagcgcccg agctttctcg atcagcggcg cgggaatcgg ggagccgccg cctcccacgg
     2761 accggaacga gctgaggtcg tagtccccga cactggggtg attgagcaac ctgtgaagca
     2821 cggtcgccgt gtatccccag cccgtgacct tctcgcgctc gatcaattcc agggtcacct
     2881 cggcgtcgaa gcggcccatt ggccagaccg ttttggcgcc acctgcgagt gccgtcaccg
     2941 ccgcgcaatg caaacccgac acgtgaaaga gcgggctcgt cacgagaatg ctgttcgcca
     3001 gctgtggcag ctccgactgc cgggggttcg ccatcatcag tcgcgcgccg tggaagaagc
     3061 tgaccatgag catgttagtc acgtttccgt gcgtgtgaac gacacccttc gcgcgcccag
     3121 tcgtgccgct ggtgtacaag atgatcgccg gatcgctctc tgcgatcggc tgcgtcggaa
     3181 gctcggcctc cggatgcgcg gagaccagct catcgaaccc ttcctccacg acgagcatgg
     3241 gcacgtcgag ctcatgagta atccgggctg cccgcttgcg atcggcgatc agcacctttg
     3301 gtgtgctgtg tccgagtgca taacggatct cgtcctcggt ccaccagcca ttgagtccca
     3361 cgcagatcgc cccgaggctc acggtcgccc agaacgaaat gatccattct gggcagttcg
     3421 ccgcgaggat cgccacgcgg tcaccgggcc cgacgccaaa gcgttcggag aggcctgcgg
     3481 ccacgcgtgc aaccagcttt tcatgctcgc cgaacgtgta ccgccgctcc tgctcagcgg
     3541 cccgaaacac catgtactct ttgcccgaga actcccttgc gcgaagcagg acatcgcgaa
     3601 gcgaacgcgc acgattggcg aacacgcgaa ccttctcgcc gagaaactct tcttccgcga
     3661 gctcgaaggg agcacctggc ccgagcagct tcgcctcgac ggccaggagg cggctcatga
     3721 cgccagcttc tcctgaagct gatcatgcag ccatgcttcc gccgaggaga ggtctcgaaa
     3781 gatcttcact gggatcgggc ttggcgtgac ccaggagatc gcggtccaga ggccctgctg
     3841 aagtgcgctg gagaaaatca gtgcaccgcc cgcgcagttc atcttgaggt gctgccggtc
     3901 gcggttgatc cattccatga tttccctacg ctgcgtcgga ggcacacggc cgacctcgcg
     3961 tgcatcgatc accacggcat acggatctcc gcgacgcgcc caatcggaca tgacgtcgac
     4021 gtgccgcttc cattcggcgt catcgcacaa accctggtaa gtcatgcgca tcagcggctt
     4081 gtctttctcg tcgaagatga ctcggcccat tttccccctc gaaaaagtct actcagctac
     4141 ttcgagcact acaacgtgta accgccatcc acgaacaagg tttgaccggt gatgtatcgc
     4201 gcgcgcgtgg atgccaggaa tacggcggcg tcggcgatct cctcgggctt cccaaatctc
     4261 cgtagcggcg tgttgcgtat cgctgcgtcg atccaaccct ggctgagctc gccgcgctca
     4321 acgagcttcg ggaacattcc cgcgtcgacg acgccgaccg caacattgtt ggctcgaaca
     4381 ccatgacgcc cctcctcgcg tgcgatgccg cgcatcagcg cctcgattgc gcccttgggg
     4441 gccaccgaaa gcacgtcgcc tgggggaaag cgtttcaagc ccgccgagct cacgaacacg
     4501 atcgagccct tgctctcgcg aaggtgcggc agagcagtgt gcaccacgtg aaaaaaaccg
     4561 ttcacgtccc agtccatggt gtttcgccac tgctccggag tgacctgact gacgtagggc
     4621 tggtcgatgt gggtccccgc cgcgtgagcg acggtgtgga tctgcccgtg ctcggcgacg
     4681 agcccatcca tgcaggcgcg gaccgattcc aaatcgccga gctccacccg atgcaccgag
     4741 caggttccgc cgccacttcg aatctcatcg gcgacggcat tggccgcatc ctcgttgtgg
     4801 cggtacgtga tggcgagcgt gacgccggcc gccgcgaagc cgcgcgcgat cgcgcttcca
     4861 atgccaccgc ttccgccgat gatgaggcct gcacccggtg ggaaatcaag caccggcgcg
     4921 ctcccgaagc tcgaacttca ggaccttgcc cgtcgcgttc cggggcagtg ctccgaggat
     4981 ctcgacgcgc cgcggcactt tgaagtttgc catgttctcc cggctccatg cgatcactgc
     5041 ttccggtgcg agcacctctc ccggcgccgg aaccacgaac gccatgccga cttctccgag
     5101 tcgctcatcg ggtacgccga tgacggctac ctcgcccaca cccggcattt gcaagagagt
     5161 gttttcgatc tcggcgggat aagcgttgaa cccaccgacg atgaacatgt ccttgagccg
     5221 gtccgtgatc ttcacgtacc cgcgctcgtc catggtgccg atatcgcccg tgtgaagcca
     5281 gccatcggga tcgatcgcct ctctggtctc ttcttcggcg tcgaagtagc ccttcatgac
     5341 gttgtagccc cggaccacga tctcgccggg ctcgtttgcg ggcatcgaat tgcctgcatt
     5401 atccacgatg cgaacttcga cgtcgggaat cgcgcgaccg ctcgtggtgg cgatggtctc
     5461 ggcatcgtcg tcgagacggc acatcgtcac cacgccacag ctctccgtga ggccgtacgc
     5521 ggtgatcacc gtttcaaatc ccagctcgtc tttcatgcgc tgaatgagct cgaccgggat
     5581 cgccgcggcg cccgtcaccg ccagccgcag gctcgaaatg tcgaacttgg cgcgatcggg
     5641 gtggagcaga atggactgat agagggcagg gggacccgga agcatcgtga tcgcatcctc
     5701 ctcgattcgc cggagcacca cgttcacgtc gaacaagggc tcagggtaga tcgtggcgcc
     5761 ccgcagaagc gccgagaacc agccggcctt gtaaccaaag ctgtggaaaa acggtaggac
     5821 gatcaagtac cggtcgccct cgcgtagacc gacgatcgag ctccactgat cgaagacgcg
     5881 gaggttctgg gcgtgagtgc acatcgcgcc cttgggcctt cccgtcgtac ccgaggtgaa
     5941 caggatgtcc gcgatgtcat ctggacggat cgttgcagcc cgtgttttgg cggcgcttgg
     6001 cgaaacctct tctgcgcgtt cgaggaaggc ttcgtagctg agcgtgcctt cgggggcctc
     6061 gccacggagt acgatgatct gcgcgagctc tggtacctcg gctggcgcgt tgcggagcaa
     6121 ggccacgtag tcgacatcga ggaagcccgt cacggtgaaa agtgccttcg cgccgctctt
     6181 ctcgagcaga taggcggcct cgatcccttt gtagcgggtg ttgacgggca ccatcacgcc
     6241 accgacggtg tgaatcgcta acgcggcgac gatccactcc cagatattgg gcgcccaaat
     6301 tgcgacccga tcgccacttt cgatacccag cgcgatcagg gccctcgcgg cctcgtcgac
     6361 ccgggccgcc aggtccaaga agctcagcga ttcgtcgcca tcgacgatgg cctttgtttt
     6421 ccccagggcg tcggcctttt gggcaagcat atcggggatg gttccagcca ccgggctctt
     6481 gatatcgcca agccaggatg ggggcaacgg gccgtctcgg agaattcatc tcagccatcc
     6541 ccattctcgg attgaccagc gcccggatat gaacatgttt gccccgaaat tggggggatc
     6601 ttccttttca tgaggaatgc tcatttcttc gactagatga atcttcaacc cacaattgcc
     6661 cctgaacact ctaggagaag gcttagatta tggacaggga ttggagtgta gatccgacct
     6721 atcggtcgga gcgcttagtg atcgatggga cactttctcc gatcgtgctt tacgacatgc
     6781 gactcccgcg tcaggtggag gatgaggaga tcgcgctcat gctca
//
LOCUS       DASRZF010000126        15311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3524612, whole genome shotgun sequence.
ACCESSION   DASRZF010000126 DASRZF010000000
VERSION     DASRZF010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 15311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15311
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3524612"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1899
                     /locus_tag="VFG22_05825"
                     /pseudo
     CDS             1..1899
                     /locus_tag="VFG22_05825"
                     /inference="COORDINATES: protein motif:HMM:NF021424.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
     gene            complement(1906..2649)
                     /locus_tag="VFG22_05830"
     CDS             complement(1906..2649)
                     /locus_tag="VFG22_05830"
                     /inference="COORDINATES: protein motif:HMM:NF012452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415794.1"
                     /translation="MAVKVDEGLVFASDSRTNAGVDHISTYAKMHTFCGDGTRFFALL
                     SAGNLATSRAVVTHLQRDIEERAEVNLETIGRLTEAADYIGRVSREQQQKHSKGARGP
                     QYDSGASFILGGQIQGQPPSILLIYPEGNYIPNSRHSPFLQIGEEKYGKPILDRIIQP
                     GVSLEVAARCALVSMDSTMRSNASVGPPIDLLLYSTGTQRPGSMVTFGEDDDYLRVLR
                     RSWQDSLKASFNQLPKIPLPSASVRLVDA"
     gene            2809..3618
                     /locus_tag="VFG22_05835"
     CDS             2809..3618
                     /locus_tag="VFG22_05835"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HET6415795.1"
                     /translation="MILDRFKVDGRVAVVTGAGRGIGRGISIALAEAGADLVLASRRE
                     HVLEEVAGIVRQNGRRALVVPTDVFDAGQLDRLAERAIAEFGGVDFLVNNAGGTLPGP
                     ALQCTDEELDKSFHFNVTTALHMSRSLAPSMAERGHGAIVNITSGLSHLVDHGFVAYG
                     TAKAAMSHMTRLLAHEWAPHVRVNAIAVGATRTDALAMVTADKSLANQMVELTPLRRL
                     GTVEDIGAAALYLLSEAGSWVTGKVLEVDGGTVDSNWPFAMPSGLRGPKGT"
     gene            3622..4677
                     /locus_tag="VFG22_05840"
     CDS             3622..4677
                     /locus_tag="VFG22_05840"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HET6415796.1"
                     /translation="MKSAVIDAFSDDASGVEVRDVPAPRPGPGQLRVRMRMAPVNPSD
                     FNYIDGIYARSFSRMIWNRGSERPADAPSGDVFPMPPYSLGVEGVGVVEESGGGFLGG
                     RLVGKRVAVVSGPPNGTWQEETLIDARRAFPVPRSLSDAEACSFFVNPLTALVLVRHV
                     LQVPRGGVLLQTAAGSALAQMVRNLAREDGFRVIDVVRSRSGAEKLKQANAKYVIALE
                     DEDLLDAVHRFVPEGVDFVLDCVGGETGSEALRTLTPGGRMVCYGTLSREPITLPPRD
                     IMMPMTRIEGFYLAAYLKNRTIFQRLGLVRRTAKLIASGVLGTSVEHTYRLDDLQAAL
                     EHARRPGRTGKVLLDLT"
     gene            complement(4687..5442)
                     /locus_tag="VFG22_05845"
     CDS             complement(4687..5442)
                     /locus_tag="VFG22_05845"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET6415797.1"
                     /translation="MTEREEPSYTIDQLAALTSVPSRTIRFYQSKGALPGPVRRGRKA
                     FYGPAHIERLELIARLQDRGLQIRAIRNLLQRLDCGQASLEDWLGFEAELRTPWSEDV
                     PQLLSEQEIQERLANRPPGLLAALIKAGLVEREANQYLVHSPALMNMALTLEENGIDV
                     ETAHEATQILSRHLSKAATELSLLFHEQTHERARDGSVDNSAQLLNALKPLALEAASV
                     VFAQEIQLALEQLLHEGRLAPITAGRSAEKERS"
     gene            5799..6254
                     /locus_tag="VFG22_05850"
     CDS             5799..6254
                     /locus_tag="VFG22_05850"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET6415798.1"
                     /translation="MKPLIVVCTDFSPCSDLALERAAEIAKRDETRVFLVHTFDPRRS
                     NRPAARQVADVDAGDWDEESMHRLREARLRYFGHLEDSDVQYDAISSAHPSMEICKIA
                     KDTNASLIVLGSHGRTGVLRRLLGSVAEATVRHAPCSVFVVREPAVPAS"
     gene            complement(6282..6551)
                     /locus_tag="VFG22_05855"
     CDS             complement(6282..6551)
                     /locus_tag="VFG22_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415799.1"
                     /translation="MSAEEANKKLKQDLEDTRNDLRRAADEIKVKLHLAGMDAKDAWE
                     EIQPRLADFERRFDSKAGEVGEELKALGSEIKKRLQNIKSKIASE"
     gene            6835..8589
                     /gene="acsA"
                     /locus_tag="VFG22_05860"
     CDS             6835..8589
                     /gene="acsA"
                     /locus_tag="VFG22_05860"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HET6415800.1"
                     /translation="MRETSQVDPQVAPNMKDYDEERSSFSWNAVRAELDGLPGGRGIN
                     IAYEALGRHVKHGHGDQTAIRWLGKKGGRRTLTYSELDALSARFANVLAALGVEPGDR
                     VYSLAGRIPELYVAALGTLKHRAVFCPLFSAFGPEPIAERLNLGEARVLFTTERLYKR
                     RIEDIRHRLPSLEHVIVVAEPMKPKRPEATVDWEDAMKSATAEYEIGETDPEMPALLH
                     FTSGTTGTPKGAYHVHQAVLTHFVTGKLALDLHPGDIYWCTADPGWVTGTSYGISAPL
                     TNRVTMIVDEAEFDPQRWYRILEEERVQVWYTAPTAIRMLMKAGAELPRSKDLSALRF
                     MASVGEPLNPQAVLWGQEVLGKPFHDNWWQTETGGIMISNFASMKVKPGSMGRPMPGI
                     TAGILREEEDAKLHEVEDPAKDGELALRPDWPSVFRGYWNNQERYDACFRDGWYLSGD
                     LARRDEEGYYWFVGRSDDVIKSAGHLIGPFEVESVIMKHPAVAEVGVIGKPDSVAGTI
                     IKAFVSLQPGYEWSDALQSELIGFGRKNLGTAAPREIDFVESLPKTRSRKIMRRLLKA
                     RELGLPEGDTSTLEGGDS"
     gene            8586..9563
                     /gene="pdhA"
                     /locus_tag="VFG22_05865"
     CDS             8586..9563
                     /gene="pdhA"
                     /locus_tag="VFG22_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HET6415801.1"
                     /translation="MTLDISREHALELLRDMIRIRRLEEKSAELYSKQKIRGFLHLYI
                     GEEAVGVGAMHALREDDNIVATYREHGQALIRGIPAGEIMAEMYGKANGCSGGRGGSM
                     HLFSAKRRFYGGHAIVGGGLPVAVGLALADKMKGESRVTACFFGDGATDEGEFHESLN
                     LAALWKLPVLFLCENNEYAMGTALYRHVADRDIANNPPAYGVPADSVNGMDVIAVERA
                     VHNEAELVRETSKPRFLELKTYRFRAHSMYDAELYRSKEEVEEHKKNDPIESLKERAI
                     GDGLLTETDVDAIEADAADEIAAAIEFAEQGEWEPISEITRHTYARNLS"
     gene            9560..10534
                     /locus_tag="VFG22_05870"
     CDS             9560..10534
                     /locus_tag="VFG22_05870"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HET6415802.1"
                     /translation="MTTYRDAIRTALQTLLREDPRVFLMGEDVGRYGGAYAVSKGLLE
                     EFGEDRIRDTPLCESTFVGAGIGAALGGMRPIVEVMTVNFSLLALDQVVNNAAVMRHM
                     SGGQFEVPIAVRMATGGGKQIAAQHSHSLENWYAHIPGIKVLAPATVQDAHDMLIAAV
                     EDPDPVFIFEHQMLYGIEGSLETESTPPSPWKAAIRRPGQDLSLIAYGGSVHKALSAA
                     AELEKEGVSAEVVDLRSLRPLDRETILGSVTRTHRAVVIDEAWKTGSLASEISAILVE
                     DAFYELDAPVLRVCTLEVPIPYAKHLEEAAIPQVDRVVEAAKRSMAGG"
     gene            10527..11810
                     /locus_tag="VFG22_05875"
     CDS             10527..11810
                     /locus_tag="VFG22_05875"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HET6415803.1"
                     /translation="MAEFVMPSLGADMTAGTLAKWRVVAGDRVSQGDIIAEVETDKGL
                     IEVEVFESGRVEKLLVEEGAKVPVGTPLAEISSVSATVPESAPPPEPGGIISSGASNP
                     RLDGPMASPAVRRRAHELDVPLDGVRGSGEHGRITLEDLRGVTRGAESSPDRVRISPY
                     ARRLAIEHGLDLSQLRGTGPAGAIQASDVLGAKLSRPGSSQAAPETRAKRMQRAIAAA
                     MTRANDEIPHYYVEQSIDMGRALDWLRDTNTSRSVSERLVSGVLVLRAVASALRKHAA
                     LNSTWQVGEAVPSAQVHVGLAVSLRGGGLVAPALQNTDEGSLDDLMTRMTDLVSRARA
                     GALRSSELTSATITLTSLGERGVESVTPIIFPPQVAIVGCGAILERPWVVDGQVIPRP
                     IMRVTLGADHRVSNGHRGARFLSTLDRFLQEPEKL"
     gene            11807..12049
                     /locus_tag="VFG22_05880"
     CDS             11807..12049
                     /locus_tag="VFG22_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HET6415804.1"
                     /translation="MTRDEIRTALLKCLSEVAPEIANEEIEDDADLRDELDLDSMDVL
                     RWVQGIHKALSVEIPEEDYGKIASLEDAITYVASRI"
     gene            12030..14756
                     /locus_tag="VFG22_05885"
     CDS             12030..14756
                     /locus_tag="VFG22_05885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HET6415805.1"
                     /translation="MLQAVSESEPAPWSVDTQRLLASLDVSPSEGLSPREVAERQERF
                     GPNILAEFRAVSWFTILWRQLRSFVVYLLIAGSVLSFALGDHLEGVAILVVIVINILI
                     GFVTELRAVRSTEALRQLGSTETTVRRGGVAQRVSAENLVPGDIVLFEGGDVVTADVR
                     LLAASRVQSDESLLTGESVPVEKTIEVLPEETLIAERDNMLFKGTAITKGNAEGVVVS
                     TGMQTELGRITALVQKAADKKTPLEERIDSLGKVMGWLCIALVTVIGILGVLSGKEIT
                     DVIKTAIALAVATVPEGLPIVATLALARGVLRMARRNALVEQLSAVETLGSTSVILTD
                     KTGTLTENRMTVTGVIVPRQSAEDPATIGIPLPSEPAETDDDVRRLIVAAALCSDASV
                     GTDGSGQEVRVGDPMEIALLLASREIGLDHEQVLRDEPRISEEAFDPDIKMMATYHRR
                     GDAMRVIVKGAPEAVMGRCVSVRTTEGAEPLNDSTLEVWRKENHRVASDGLRMLAIAE
                     RVDQSHDGDPYRGLTLLGLVALSDPPRSDVIEAIQECHRAGVDVVMVTGDQAPTAEHI
                     AGAVRLIPAEDKGHAVTGAELQALLDGGAEGAAKLRATHVYARTDPEQKMKLLSFFQG
                     EGETVAMIGDGVNDAPALRQSDIGVAMGKRGTQVAREASDMILQDDHFATIVVAIRQG
                     RIIFRNIRTFVFYLLSCNLSEIITVGLASAVNAPLPILPMQILFLNLVTDVFPALALG
                     VGESGPFIMEHPPRPKSEALLAPRHWARLVGYGLVMSAAVLAGLAISMLVFDMNVKRA
                     VTVSFLILAGAQLGHVFNMVSPRSGFIYNEVTRNPWIWGAVILCTGLLVAAVYVPIFS
                     EVLGTEEPGREGWMLVLTLSFAPVLVGQSMRAISQIRRRYGHQVDPERGGIG"
     gene            14776..15174
                     /locus_tag="VFG22_05890"
     CDS             14776..15174
                     /locus_tag="VFG22_05890"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET6415806.1"
                     /translation="MDKTAPIADFMTSMPHTVGSEQTITFAQKLMEKHGVRHLPVLHG
                     GELYGIVSDRDLGMVAGLNEVNPDVTTVEEAMTQEAYTVSKDKPLFAVLEEMLEHKYG
                     SVVVVEGVKIVGILTTHDALQLLVDNFGRA"
BASE COUNT         2762 a         4592 c         5081 g         2876 t
ORIGIN      
        1 atgcaggtct acggaaagca gtacccgggc gaggccttgc cgcgatgggt cgtcctgact
       61 ttgcatcggg aggagggcgc tccgctctgg agccatcccg agatgttgct gctcgacgat
      121 ctgcccggtc ccaatgacga cactgatgcg cggcaactga tggatgccat cgccggtcgg
      181 ttggcgctga gcgcgtatgt gctgcccgcc gtcgaggaga gcgccgccga ttcgatcgga
      241 gggtgggtgc ttccgctcga ccggagcgac gagggttggg tgtccgaaac ctggccgttt
      301 tccgaatcgg agccgatgca gctcaccccc ggaataagcc cgatagggct tcgcttgcca
      361 ctcgcagagc ttgctgagga tcaactgcgc cgtgcgctta ccgtagaggc cacggatggc
      421 gggcttcatg ttttcattcc gccgctcgac ttcgacgcct tcgcggcgct cttgcgagtc
      481 gtcgaagagg ttgcatcgag cagcggtgtt cgcggactcg tgatttgcgg ataccgtccg
      541 agcgggtcga aaggagtcac gagcctcggg cttgcggcgg accccggggt cttggaggtg
      601 aaccttccac cgagccgaac gtggcgcgca tacgatgaga cgctcaccgc gattacaaaa
      661 gcggcggcgc aggaagggct cttcacgagc cgccaccacc tcaacggcca agtgcagggc
      721 accggcggcg gtgcacacgt gctcttcggc gggcccagtg tggacgcgaa tccatttttc
      781 gcgcggcccg atctgctggc aagtgtcatt cgctattggc agcggcatcc ttcgctttcg
      841 tacgcgttct ccggacagta tgtggggcct ggttcataag cgccgcggtc cgacgaaacg
      901 cttaccgcca ggctctacga gctcgaaacg gcgtgcctcg gcgtcgattc gttgaaggcg
      961 acgcccgagc gggacttcct cgaccggttg ttccgcaacc tgatgaccga cagtggtggc
     1021 aacacgcatc gcgcggagct gtgcctcgac aagctctgga acttcgattc gcctgcgggc
     1081 cttcaagggc tcatcgagtt gcgcgcattc gaaacgatgc cggatgtcgc gcagcagtcg
     1141 cttgcggcgc tcttcgtccg ttcgattctg gcgatgctgg cgggtgaccc ttgccgggcg
     1201 tctctcgtcc ggcacggagc tcgattgcac gatgcgttca tgctccccag cgtgctcgag
     1261 cgcgatctgg cggagatctg cgcggacctg caggcgagcg ggctcccatt cgatccggat
     1321 tggtttgagc cgatgctcga gcatcgcttc ccagtgctcg gtcgcttggc ggtggggaag
     1381 ggcgagttgc tcgttcggca agcactcgag ccctggcccc tgctggcgga gatcgccgat
     1441 ggcggaacca cttcacgcgt ggtcgacaac tcgacggacc gaattgagct ctcattgagc
     1501 gacccggtcg agcttggcga tggtcgtgtc ttggtcaacg gtgtggccct cgcgtttcgc
     1561 gcggtcggcg acgattgggt ggccggggtg cgctacaaat ctgcgagcgg ctggcctgcg
     1621 ttccatccgc acatccccgt tcaatcgccc ttgcgcatcg aggtcatcga tgcatcgggc
     1681 cggcttctag cggcggctcg ctaccatttc tggaaccccg acgcgccccg atatgaggga
     1741 cggccccgga acctcgagga cgcgaaagcg cgcgcccgga agcgctggca gctgtccgac
     1801 gtggagaagg gcgcaagcag aatcgcagtc gaaccgcggt acttcgagga atccgagtat
     1861 acgctcgacc ttcgccggca ggccggaact gctcgatagt ttcgatcagg cgtcgacgag
     1921 ccgaacggaa gcggagggca aggggatctt cggaagctgg ttgaaggacg ccttgaggct
     1981 gtcctgccaa ctacgcctca gaacgcgaag atagtcgtcg tcctcgccaa acgtcaccat
     2041 gctccccggg cgctgggtcc ccgtcgaata gagcaggagg tcgatgggag gaccgacgct
     2101 tgcgttgctg cgcattgtgg aatccatgga gacgagcgcg cagcgggcgg cgacttcgag
     2161 gctcacaccg ggctggatga tgcggtcgag gatcggcttc ccgtacttct cctcgccgat
     2221 ctgaagaaac ggcgaatgac gagagttcgg gatgtagttg ccttccgggt agatcaataa
     2281 gatcgaaggc ggctgccctt gaatctgtcc gccgagaatg aagctcgcgc cggagtcgta
     2341 ctgtggtcct cgtgcgccct tgctgtgctt ctgctgttgc tcgcggctca cccggccgat
     2401 gtagtcggcg gcttcggtga ggcggccgat cgtttcgagg ttcacctcgg cgcgctcttc
     2461 gatgtcgcgc tggaggtgcg tcaccaccgc gcggcttgtg gctaggtttc cggcgctgag
     2521 gagggcgaag aaacgcgtcc catcgccgca gaaggtgtgc atcttggcgt aggtgctgat
     2581 gtggtcgaca ccggcgttgg tcctggagtc ggacgcgaac acgagcccct catcgacttt
     2641 cactgccaca caatacgtca ttcggcggaa aatacgggag cggtcgaggc gggtcaacgc
     2701 aactgccaac gaactctgca aaccacgcgg aatgcgcccg ttcgcgagat accctggttg
     2761 cggaacgagg ccgaatgccc gaaagtaccg gccggagaca acgggctgat gattttggac
     2821 cgctttaaag tggacggccg agtggctgtc gtgacgggcg ctggccgggg tatcggccgc
     2881 gggatttcga ttgcgcttgc cgaggctggg gccgacctgg tgctggcctc gcgccgggag
     2941 cacgtgctgg aggaggtcgc cgggatcgtc aggcagaatg gacggcgcgc cttggtggtt
     3001 cccacggatg tgttcgacgc tgggcaactc gaccggctgg ccgagcgtgc gattgcagag
     3061 tttggtggtg tggatttttt ggtgaacaac gccgggggaa cgctccctgg gccggctctg
     3121 cagtgcactg atgaagagct cgacaagtcg tttcacttca acgtgaccac cgcgcttcac
     3181 atgtcgcgct cactagcgcc gtcgatggcc gaacgcgggc atggggccat cgtgaacatc
     3241 acctcagggc tttcccatct ggtcgaccat ggcttcgtcg cttacgggac ggcgaaggcc
     3301 gcgatgtcgc acatgacccg cttgctcgcg cacgaatggg cgcctcatgt tcgagtcaac
     3361 gccatcgcgg ttggggcgac gcggaccgat gcgctggcga tggtgactgc cgacaagtcg
     3421 ctggcaaatc agatggtcga gctcacgccg cttcgtcggc tcggcacggt cgaggacatc
     3481 ggcgctgcgg cgctctacct gctttcggaa gcggggagct gggtgaccgg caaagtgttg
     3541 gaagtcgacg gaggtacggt ggattccaac tggccgttcg cgatgccgag cgggctgcgc
     3601 ggcccgaaag gaacctgagc catgaagtcc gccgtcatcg atgcgttttc ggatgatgca
     3661 agcggcgtcg aggtccgcga tgtgccggcg ccgagacccg gtccaggtca gctgcgcgtg
     3721 cgaatgcgaa tggcgccggt caatccatcg gacttcaact acatcgacgg gatctatgcg
     3781 cgctcctttt cacgaatgat ctggaaccgc gggtcggagc gccccgcgga tgctccgagc
     3841 ggggatgttt tcccgatgcc accgtattcg ctcggcgtcg agggcgtcgg tgtcgtggag
     3901 gagtcgggtg ggggctttct cggcggacgc ttggtgggca agcgtgttgc cgtggtgagc
     3961 ggtccgccca acggcacctg gcaagaggaa acgctcatcg acgcgcggcg cgcgtttccg
     4021 gttccccggt ccctcagcga tgcggaggct tgctcgttct tcgtgaaccc gctcactgcg
     4081 ctcgtcttgg tgaggcatgt tctgcaggtt ccgcgcggtg gggtgcttct gcaaacggca
     4141 gccggttccg cgctcgcgca gatggttcga aatctcgctc gcgaggatgg ttttcgtgtg
     4201 attgacgtgg tgcgcagccg ctcgggcgcg gagaagctca agcaggcgaa cgccaagtac
     4261 gtcatcgctt tggaggatga agacctgctc gacgccgtgc accgattcgt gccagagggt
     4321 gtcgacttcg tcctcgactg tgttggtggc gagactggat cggaagcgct tcgaacgctg
     4381 acgcccggtg ggcgcatggt ttgttatggg accttgagcc gagaacccat cacgcttccg
     4441 ccccgcgaca tcatgatgcc gatgactcga atcgaaggct tttatttggc ggcgtatctc
     4501 aagaaccgga caatttttca gcgcctgggg ctggtgcgca ggactgcaaa gctgattgcg
     4561 agcggcgtgc tcggcacttc ggtcgaacac acttatcggc tcgacgattt gcaagcggcc
     4621 ctggagcatg cgcggcgacc tgggcggacc ggcaaggttc tgctcgactt gacttgagct
     4681 cgtccgctag ctgcgctctt tttcggcgct gcgaccggcc gtgatcggtg cgagacggcc
     4741 ttcgtgcagc agttgctcga gtgcgagctg gatttcctgt gcgaagacga cggatgcggc
     4801 ctcgagtgcc agcggcttta gcgcgttcaa gagctgcgcg gagttgtcga cgctgccatc
     4861 ccgggcgcgc tcgtgcgtct gttcatggaa gagaaggctc agctccgttg cggctttcga
     4921 tagatgacgc gacaggatct gcgtggcctc gtgcgcggtc tcgacgtcga ttccattctc
     4981 ctcgagggtg agcgccatgt tcatgagcgc ggggctgtgc accaggtact gattggcttc
     5041 gcgttccacg aggcctgcct tgattagggc ggcgagtagg ccagggggcc tgttggccaa
     5101 tcgctcttgg atttcctgct cgctcagtag ttggggcacg tcctcgctcc agggcgtgcg
     5161 gagctcggct tcgaagccga gccagtcctc gagtgaagcc tggccgcagt cgagacgctg
     5221 caagagattg cgaatcgccc ggatctgaag accccggtct tgtagacgtg cgatgagctc
     5281 caagcgctcg atgtgggcgg gaccgtagaa cgccttccga ccgcgccgca ccggtccggg
     5341 gagcgcgccc ttgctttggt agaaacggat cgttcggctc ggcacgctcg tgagagcagc
     5401 gagttggtcg atggtatagc tcggctcttc tcgctcagtc atggcgcaag gccgggatct
     5461 cggccctcgg ggacgagatt atgcgcgtgc ccggccgcag ccgcgagcga cttggccgta
     5521 gagcatgccc ggaagaaagg ccgcagcggc gaaagccgtc catgcaccga gggtgatcca
     5581 gatgaattcc attgtgcacc tcttattgta tcattcataa ctatgccagt cgtgagtgtc
     5641 aagtttgtcc caacctggga agttgtttct gggaggctcg gcagcgatgg agttgagcaa
     5701 ttgcaagtgc ttagcgaggc tataggctca gggatagggg cggagaccgc tggcccttgc
     5761 cagaacggcc gaggccgggc acgttcgccc cttcggaaat gaagccgctc atcgttgtct
     5821 gcacagattt ctcgccctgt tccgaccttg cgctcgagag ggccgccgag atcgccaaac
     5881 gggatgaaac ccgcgtcttt ctcgttcaca cgttcgaccc gaggcgctcg aatagacccg
     5941 ccgcccggca ggttgccgac gtggacgcag gtgactggga cgaggagtcc atgcatcggt
     6001 tgagagaagc gcggcttcgc tatttcgggc acctcgagga ttccgacgtg cagtacgacg
     6061 cgatttcgag cgctcatcca tcgatggaga tctgcaagat cgcaaaggac acgaacgcca
     6121 gcctcatcgt gctcggctcg cacggccgca ccggcgtctt gcggcgccta cttggaagcg
     6181 tcgctgaagc caccgtgcgt catgcgccgt gcagcgtgtt cgtcgtgcgc gaaccggccg
     6241 tgcctgcttc ctaggagccc ccgatgggtc ggtcccttga gctattcgga cgcgatcttg
     6301 gacttgatgt tctggagccg cttcttgatc tcgctgccca gtgctttcag ctcctcgccc
     6361 acctctccgg ccttcgaatc gaaacgccgc tcgaagtctg cgaggcgtgg ctggatctcc
     6421 tcccaggcgt ctttcgcatc catgccggcg aggtgcagct tcaccttgat ttcgtccgca
     6481 gcccggcgga ggtcatttcg ggtgtcctcc aggtcctgct tgagtttctt gttggcttct
     6541 tctgcgctca tggtctcctc cttgaggggt ccgaggtcat acccgtagtg cagccaccgt
     6601 gccagatgga tcccctgtga attcgaggta tcgggagcgc agccggcgca gtttcttcgc
     6661 caacggctta gtgatacgca cgattggcgc ctccctcgcc ctctgaccct ccgaaatccc
     6721 tagtctcgtg tgtgcagagc gctgcggtcc acgttggcgc gccagatgca tgcaagcccc
     6781 aggcatgctc gcaagcacct cggagctcgc gacggtgccc tgggaaaccc cgccatgaga
     6841 gaaaccagcc aagtcgaccc gcaagtcgct cccaacatga aagactacga cgaggaacgt
     6901 tcgtcgtttt cgtggaatgc ggtgcgggcg gagctcgatg gccttcctgg cgggcgtgga
     6961 atcaacattg cgtatgaagc gctcgggcgg cacgtgaagc atgggcacgg cgatcagacc
     7021 gcgatccgat ggctcgggaa gaagggcggg cgccggacgc tcacatattc tgagctcgat
     7081 gcgctcagtg ctcgcttcgc gaacgtgctc gcggcgctcg gtgtcgaacc cggcgatcgg
     7141 gtttactcgc tcgcagggag gattccggag ctctacgtcg cggctctggg cacgctcaag
     7201 catcgggccg tgttctgtcc gttgttctcg gcgtttggac cggagcccat tgcggaaagg
     7261 ctcaacctcg gagaggcccg tgtgctcttc acgacggagc gcctctacaa gcgccgcatc
     7321 gaggacattc gccatcggct tccaagtctc gagcacgtca tcgtcgttgc ggagccgatg
     7381 aagcccaagc gacccgaagc gaccgtcgac tgggaagacg cgatgaagag cgcgaccgcg
     7441 gagtacgaga ttggcgaaac cgatccagaa atgcctgcgc tgctccactt caccagtggc
     7501 acgacgggca cgcccaaggg cgcctatcac gtgcatcaag cggtgttgac tcacttcgtg
     7561 acgggaaagt tggcgctcga cttgcatccc ggtgacatct actggtgcac ggccgacccc
     7621 ggttgggtga cgggtacttc gtacggaatc agcgcgcccc tcaccaatcg ggtcacgatg
     7681 atcgtcgacg aggccgagtt cgatccacag cgctggtatc ggatcctaga ggaagagaga
     7741 gtgcaggtct ggtacaccgc tcccacggcg attcggatgc tgatgaaggc cggggccgag
     7801 cttccgagga gcaaggacct ttctgcgctt cgtttcatgg ccagtgtggg ggaaccgctg
     7861 aatccgcagg cggtcctctg gggtcaggaa gtcctcggaa agccgtttca cgacaattgg
     7921 tggcagacgg aaaccggcgg gatcatgatc tcgaacttcg cctcgatgaa agtgaagccc
     7981 ggctcgatgg ggcgtccgat gcccggtatc acagccggga tcctacgcga agaagaagac
     8041 gcgaagctcc acgaagtcga ggatccggcg aaggacggcg agctcgcctt gcggcccgac
     8101 tggccctcgg tgtttcgcgg gtactggaat aatcaggaac gctacgacgc atgctttcgc
     8161 gatggctggt atctctccgg cgacctcgcg cggcgcgatg aagagggata ctactggttc
     8221 gtcgggcgct cggacgacgt gatcaagtcc gcaggacacc taatcgggcc gttcgaagtc
     8281 gagagcgtca tcatgaagca tccggcggtc gccgaagttg gagtcatcgg caagcccgac
     8341 tctgtcgctg gcaccatcat caaggcgttc gtgagccttc agcccggcta cgaatggagc
     8401 gacgcgcttc agagcgagct catcggtttc gggcgcaaga atctcggaac agcggctccg
     8461 cgtgaaatcg acttcgtcga gtccttaccc aagacgcgga gcagaaagat catgcgacgc
     8521 cttctcaagg cacgagagct cggcttgccc gaaggggata cgtcgacttt ggaaggagga
     8581 gacagttgac gctcgatatc tcacgtgagc atgcgctcga gttgctgcgg gacatgatcc
     8641 gcatccgccg gctcgaggaa aagagcgcgg agctctactc gaaacagaag attcgcgggt
     8701 tcttgcacct ctacatcggc gaagaggctg tgggggttgg cgcgatgcat gcgctccggg
     8761 aagacgacaa catcgtcgca acctatcgcg agcacggtca ggcgctcatt cggggaatcc
     8821 ccgccggcga gatcatggcc gagatgtacg gcaaggcgaa cggctgctct ggagggcgtg
     8881 gtggctcgat gcacctgttt tcggcgaagc gacggttcta cggcgggcac gcgatcgtgg
     8941 gcggaggctt gccggttgcg gtgggcctgg cgctggccga caagatgaaa ggcgagtcgc
     9001 gcgtgaccgc ttgcttcttt ggcgacggtg caaccgacga gggcgagttc cacgagtcgc
     9061 tgaacctagc ggcgctttgg aagctgcccg tcttgttcct ctgtgagaac aacgagtacg
     9121 cgatggggac ggcgctctac cgtcacgttg cggaccggga catcgcgaac aatccgccag
     9181 cgtacggagt ccccgcagac tcggtcaacg gcatggacgt gatcgccgtc gagcgcgctg
     9241 tgcacaacga agcggagttg gtgcgcgaga cgagcaagcc gcgattcctc gagctcaaga
     9301 cctatcgatt tcgcgcgcac tccatgtacg acgcggagct gtaccgctcg aaagaggagg
     9361 tcgaagagca caagaaaaac gacccgatcg agagcttgaa ggagcgcgcg atcggagatg
     9421 gactgcttac ggaaaccgat gtcgatgcaa ttgaggccga tgccgccgac gagatcgcgg
     9481 cggcgatcga gtttgcagag caaggcgagt gggagcccat ctccgagatc acccgtcaca
     9541 cctatgcgag gaacttgtcg tgaccacgta ccgagacgcg atccggacgg cgctccaaac
     9601 cctgcttcgc gaagatccgc gcgtgttcct catgggtgag gatgtcgggc gctatggggg
     9661 ggcctatgcc gtgagcaagg ggctgctcga agagtttggg gaagaccgca ttcgcgacac
     9721 gccactctgc gagtcgacgt tcgtcggcgc gggaatcggc gctgcgctcg gtggaatgcg
     9781 ccccatcgtc gaagtgatga cggtcaactt cagcttgctt gctctcgatc aggtcgtgaa
     9841 caacgccgcc gtgatgcggc acatgtccgg cggacagttc gaagttccga tcgccgtgcg
     9901 catggcgaca ggtgggggca agcagatcgc ggcgcagcac tcgcatagtc tcgagaactg
     9961 gtacgctcac atccccggca tcaaggtact tgctccggcg accgtgcaag atgcgcacga
    10021 catgctgatc gcggccgtcg aggatccgga cccggtgttc attttcgagc accagatgct
    10081 ctacggaatc gaagggtcgc tcgagacgga gtcgacaccg ccgtctccgt ggaaggccgc
    10141 gattcgtcgc cccggtcagg acctcagcct catcgcgtac ggcggatcgg tgcacaaggc
    10201 ccttagcgcg gccgcggagc tcgaaaagga aggggtgagt gcggaggtcg tcgatttacg
    10261 aagcctgcgg ccgctcgacc gcgaaacgat cctcggcagc gtgacgcgca cgcatcgcgc
    10321 cgtggtcatc gacgaggcct ggaagaccgg aagcctggct tcggagatca gcgccattct
    10381 cgtcgaggac gcgttctacg agctcgacgc gcccgtattg cgcgtgtgca ccttggaggt
    10441 gccgatcccg tacgccaagc acctcgaaga ggcggccatc ccccaggtcg accgggtcgt
    10501 cgaagcggcg aagaggagca tggccggtgg ctgagttcgt catgccttcg cttggcgccg
    10561 acatgacggc aggcacgctg gcgaagtggc gagtggtggc tggagatcgt gtctcgcaag
    10621 gcgacatcat cgcggaggta gaaaccgaca agggtctcat cgaggtcgag gtcttcgaaa
    10681 gcgggcgggt cgaaaagctc ttggtcgagg agggggccaa ggtgccggtg gggaccccgc
    10741 tggcggaaat ttcgtcagtg tcagcgacag tgccggagtc ggcgccaccg cccgagccgg
    10801 gcggcatcat ttcttccggt gcttccaatc ccaggctcga cgggcccatg gcgtcgcctg
    10861 cggttcggcg gcgcgcgcac gagctcgacg tgccgctcga cggggtcaga gggagtggcg
    10921 agcatgggcg gatcacgctc gaggacctgc gaggcgtgac ccgcggcgcg gagtcctccc
    10981 cagaccgcgt tcgcatttcc ccttatgccc gtcgcctcgc aatcgagcac ggcctcgatc
    11041 tcagccagct ccgaggaacc ggtccagccg gggcgatcca ggccagtgac gtgctcggcg
    11101 cgaagctgag ccgaccgggc tcgagccaag ctgctcccga aacgcgggcc aagcgcatgc
    11161 agcgggcgat tgcagctgcc atgacacgcg ccaacgatga gattccccac tactacgtcg
    11221 agcagagcat cgacatgggt cgcgcgctcg actggctgcg agacaccaac acgtctcgga
    11281 gcgtgagcga gcgactggtg tcgggggtac tcgtcttgcg ggccgtcgcc agtgcactcc
    11341 gaaagcacgc tgctctcaat tcgacttggc aagttgggga agcagtgcca agcgcgcagg
    11401 ttcacgttgg gcttgcggtg tcccttcgcg gtggcggtct cgtggcgccg gccttgcaga
    11461 acaccgatga aggaagcctc gacgacttga tgacgcgcat gacggatctg gtgagccgcg
    11521 cccgcgccgg tgcgctccga agctcggagc tgacctccgc cacaattacg ctgacctcgc
    11581 taggcgagcg cggtgtcgag agcgtcacgc ccatcatctt tccgccgcag gtggcgattg
    11641 tcggctgcgg cgcgattctc gagcggccct gggtggttga cggccaagtg attccccgtc
    11701 cgatcatgcg ggtcacgctt ggcgccgatc acagagttag caacggtcac cggggggcgc
    11761 gctttttgag caccctcgat cgcttcttgc aagagccgga gaaactatga cgcgcgacga
    11821 aatacgaacg gccttgctca aatgcctgag cgaagtggcc cccgaaatcg cgaacgaaga
    11881 gatcgaggac gatgcagacc ttcgagacga gctcgacctc gattcgatgg acgttcttcg
    11941 ttgggttcaa ggaattcaca aggcgctgag tgtggagatc cctgaagaag actatggcaa
    12001 gatcgccagc ctcgaggatg ccattaccta tgttgcaagc cgtatctgaa tctgagcccg
    12061 caccctggtc tgtcgacacg caacgcttgc tcgcgagcct cgacgtaagt ccgtccgagg
    12121 ggctcagccc gcgcgaggtc gcggaacgcc aggagcgatt tggaccaaac atcctcgccg
    12181 agttccgtgc ggtttcctgg tttacgattc tctggaggca gcttcgaagc ttcgtggtgt
    12241 atctgctgat tgcgggttcg gtgctctcct ttgccctggg cgaccatctc gaaggcgttg
    12301 cgatcctggt ggtcatcgtg atcaacatcc tgatcggatt cgtcaccgag ctccgcgcag
    12361 tgcgctcgac cgaagcgctt cgccagctcg gaagcaccga aacgactgtt cgacgcggcg
    12421 gcgtggcgca acgcgtgtct gcggagaact tggtcccggg agacatcgtc ctcttcgaag
    12481 ggggggacgt cgtgacggcc gatgtgcggt tgctggccgc gtcgagggtg cagagcgacg
    12541 aatcgctcct gaccggggag tccgttccgg tcgaaaagac aatcgaggtg ctgccggaag
    12601 agacgctgat cgcggagcgc gacaacatgc tgttcaaggg gacggccatc accaagggaa
    12661 acgccgaagg cgtggtcgtg agcacgggca tgcagaccga gctcggtcga atcacggccc
    12721 tggtgcagaa ggcagccgac aagaaaacgc cgctcgaaga gcgaatcgac tccctcggaa
    12781 aggtgatggg gtggctctgc atcgctctgg tgaccgtcat cggaattctc ggcgtgttga
    12841 gcgggaagga gatcaccgac gtcatcaaga ccgcaatcgc gctggcggtc gcgaccgtgc
    12901 ccgaagggct cccgatcgtg gccaccctgg cgctggcgcg tggcgtcctc cggatggcgc
    12961 gccgcaatgc gctcgtcgag cagctctcag cggtcgaaac actcggctcg accagtgtga
    13021 tcctcaccga caagaccggc acgctcaccg agaacaggat gacggtcacc ggtgtcatcg
    13081 ttccacggca aagcgcagag gaccctgcga ccatcggtat tccccttcca agcgagccgg
    13141 ccgagacgga tgacgacgtc cgacgcttga tcgtcgcggc cgcgctttgc agtgacgcgt
    13201 ccgtaggaac ggacggctcg ggacaggagg ttcgggtcgg cgaccccatg gagatcgcgc
    13261 tcctactggc gtcgcgggag attggtctcg accacgaaca ggtgcttcga gacgagccgc
    13321 gcatctcgga ggaagcgttc gatccggata tcaagatgat ggcgacctac catcgtcgcg
    13381 gcgacgccat gcgcgtcatc gtgaaaggcg cgcccgaagc ggtgatgggc cgatgcgtct
    13441 ccgtgcgcac caccgaaggc gcggagccgc tcaacgattc gacgctcgag gtctggcgga
    13501 aggagaatca tcgcgtggcc tcggatgggc tccgcatgct cgcgatcgcc gagcgtgtcg
    13561 accagtctca cgatggcgat ccgtatcggg gtctgacgct tcttggcctc gtggcgcttt
    13621 cggatccccc acgctcggac gtcatcgagg ccattcagga gtgccatcga gccggcgtgg
    13681 acgtcgtgat ggtaaccggc gaccaagcgc cgacggccga gcacatcgcc ggcgccgtta
    13741 gattgattcc agccgaagat aaggggcacg ccgtcaccgg tgccgagctt caagcacttc
    13801 tcgacggagg tgcagaaggc gccgccaagc tccgagcgac gcatgtctac gcaagaaccg
    13861 accccgaaca gaagatgaaa ctactgtcgt tcttccaagg cgaaggcgaa accgtggcga
    13921 tgatcgggga tggggtcaac gatgcgccgg cgctccgtca gtccgatatc ggcgttgcga
    13981 tggggaagcg cggcacgcag gtcgcgcgcg aggcctcgga catgatcctc caagacgacc
    14041 atttcgcgac gatcgtcgta gcgattcggc aaggtcgcat catcttccgg aacatccgaa
    14101 ccttcgtctt ctacctcttg tcgtgcaatc tgagcgagat catcaccgtg ggccttgcgt
    14161 cggccgtgaa cgcgccgctt ccgattctac cgatgcagat cctcttcttg aatctcgtga
    14221 ccgacgtgtt ccccgcgctc gcgctcggcg taggggagag cggacccttc atcatggaac
    14281 atccgccccg accgaagagc gaagcccttc tcgcgccgag gcattgggca aggctcgtgg
    14341 gctacggcct ggtgatgagc gccgcggttc tggccgggct cgccatctcc atgctggtct
    14401 tcgatatgaa cgtgaagcgc gcggtgaccg tctcgttcct gatcttggcg ggcgcgcagc
    14461 tcgggcacgt cttcaacatg gtctctccac ggtccggctt catttacaac gaagtcacgc
    14521 gcaacccctg gatctggggg gccgtgattc tttgcacggg cctcctggtc gccgcggtct
    14581 acgtgccaat cttttcggag gtcctcggaa ccgaggaacc tgggcgggag ggctggatgc
    14641 tggttctcac cctgagcttc gccccggtcc tggtcggcca gagcatgcgg gcgatcagcc
    14701 agatccgtcg cagatatggg caccaagtgg accctgagcg tggcggaatc ggctgactgt
    14761 ggtagaggag cgcgcatgga caagaccgcg ccgatcgccg acttcatgac ctcgatgccc
    14821 cataccgtgg gcagcgagca gacgatcacc tttgcccaga agctcatgga gaagcacggc
    14881 gtccgccacc tccccgttct acacggcggc gagctctacg gcatcgtgag cgaccgcgat
    14941 ctcggcatgg tcgcgggcct caacgaagtg aaccccgacg tcaccaccgt cgaagaggcg
    15001 atgacccaag aggcatacac ggtctcgaaa gataaaccgc tcttcgccgt tctagaagag
    15061 atgctcgagc acaagtacgg ctccgtcgtg gtcgtcgaag gcgtcaagat cgttggcatc
    15121 ctcaccacgc acgacgccct gcagctcctg gtcgacaact tcggccgcgc gtaagcggat
    15181 acgctctccg ggtgattcgt ccgaggtccg ccggctggag tcggactcgg ggcgtagcgt
    15241 tcaacccaaa taccctgaac gcgccagtcg ctggacactg ttgacggcac ttcgagagtg
    15301 ggtttagcgt a
//
LOCUS       DASRZF010000127         5876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2493171, whole genome shotgun sequence.
ACCESSION   DASRZF010000127 DASRZF010000000
VERSION     DASRZF010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 5876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5876
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2493171"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            318..1541
                     /locus_tag="VFG22_05895"
     CDS             318..1541
                     /locus_tag="VFG22_05895"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HET6415807.1"
                     /translation="MSLVVQKYGGTSVGSLDRIRLVAARVADTYREGHQVIVVVSAMA
                     GETDRLLNLGWELSGQPAEREMDVLVSSGEQVSAALLAIQLNEIGVPALSLLGHQARI
                     LTDNVHRKARIRTIDGPGLRSSLETGNVLVVAGFQGTDAYGNITTLGRGGSDTSAVAI
                     AAAVNADICEICTDVDGVYTTDPGICPNARKLQRISYEEMLELSSQGAKVLQIRSVEL
                     AMKHRMPLHVRSSFSKEEGTIVTDEEHLLEKVIVAGVAADKKTAKLTVHALADHPGVV
                     ASVFEPLAEANISVDMIIQNVSKAGHTDLTFTVSHEDADRAEAIAKEIVASVGAEDVI
                     RDDAVAKVSIVGVGMRNHAGIAARMFRLLADNGINIQAISTSEIKTSCLIESAHADRA
                     VAVLHDGFELGNPQR"
     gene            1703..1831
                     /locus_tag="VFG22_05900"
     CDS             1703..1831
                     /locus_tag="VFG22_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415808.1"
                     /translation="MDFESNTEAQRRGVDRGVRHATDSEPEPERRLVFVYGGPGLK"
     gene            1925..2782
                     /locus_tag="VFG22_05905"
     CDS             1925..2782
                     /locus_tag="VFG22_05905"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6415809.1"
                     /translation="MANDPAENEKQETLTAGQRLAAQQAAKAARKAAQKGRDAELVEE
                     KALAQAAVAKDWLQDNLKPLGLLAGGVLLMAAIGIGWSTFNRGQQRAAGEELAAVLDG
                     GADDNAVLATAYATVADAHPKTPAAAWARIGEGRALYASGEWERARGAYQAALESSDD
                     ETVRWAALEGIAYAFEAEESYERAIEELEALRELDRNVAPIAGYHQGRILAKQGKLEE
                     AKSKFDRVLNELKEADAPLLPYTQEQTEARLALIDPSVAPAGGIDPRKADEFIRQMNQ
                     MLQRQPPAE"
     gene            2794..4011
                     /locus_tag="VFG22_05910"
     CDS             2794..4011
                     /locus_tag="VFG22_05910"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET6415810.1"
                     /translation="MRIRFGSARTAAAWVLVASFALMAIAGCGAAGTPTSNADNFGWV
                     SQGNDYAPPGEGGFNVRWSKQLTDRWEARYLPVELSSAEFDTKRERVYIGTTEGNFFA
                     FEMNGRRLFFYDAHAQIESKAGVDADTGEVFFGSVDGTVHALSVTGELKWKAKLIGSI
                     RAQPVLAAGALYVVTDTDTVTALARDDGRVLWTYDKEPVEEITIAGHAGMLLEDGRLY
                     AAFTDGAVAALNPADGSLYWELETALDVELRPGNVPQFLDVDTTPVVRQGTLYVASFT
                     AGLYALNASSGSVEWRDADFKGVTGLASVGRMLIITSAPRGVSLLDLRTREIQWEKAP
                     ERGAPTPPVVTETGTVIYGETQGSLLALSLSDGHEIARAEGGSGFSATPAAAGAFGGA
                     ISNGGRFLCLRIN"
     gene            complement(4024..4719)
                     /gene="aqpZ"
                     /locus_tag="VFG22_05915"
     CDS             complement(4024..4719)
                     /gene="aqpZ"
                     /locus_tag="VFG22_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HET6415811.1"
                     /translation="MSLGNKVAAEFVGTFWLVFGGCGSAVLAAGFPELGIGFLGVALA
                     FGLTVLTMAYAIGHISGCHLNPAVSVGLLLGKRFPASELLPYVIAQVLGGIAGAAVLY
                     FIASGQDGFQAGGFASNGFAEHSPGGYSMAAALTAEIVLTFAFLFVILGATDKRAPAA
                     LAPVAIGLCLTLIHLIGIPVDNLSVNPARSTAVAIFAEPWALQQLWLFWVAPIIGGAI
                     GGVVYPLVAGNGD"
     gene            complement(5273..5876)
                     /locus_tag="VFG22_05920"
                     /pseudo
     CDS             complement(5273..5876)
                     /locus_tag="VFG22_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567368.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ISNCY family transposase"
BASE COUNT         1150 a         1817 c         1911 g          998 t
ORIGIN      
        1 ccaaaaagcc aaccaacgag ccagcgcgtg tggatacaag tgggcatcgg gtctcctacg
       61 ggggggggcg accgtaacac agctgaacgg gcgtcgatcc acgttttttc tacggacttg
      121 gaaggattgt gaggcgtctc acagtgaggc acgggcgcct gcacccaacc catccccgaa
      181 cgggcgggac gcgaacgccg gcaccggccc tggcgctcag accgacgctg acgctgcgtc
      241 ctcgaaaaga caaaggaaac tcaagtccct ccatggcggt tgccccctac aaacccgcgg
      301 ctatccttcg gcgccgaatg agcctggtgg tacagaaata tggtgggacg agcgtcggat
      361 cgctcgatcg tatccgtttg gtcgcggcgc gcgtcgccga cacgtatcga gaaggtcacc
      421 aggtcatcgt ggtcgtgagc gcgatggcag gcgaaaccga tcgattgctc aacctgggtt
      481 gggagctttc gggacagccc gcagagcgcg agatggacgt gctcgtctcg agcggcgagc
      541 aagtgagcgc cgcgttgctg gcgatacaac tcaacgaaat cggcgtgccg gcgctttcac
      601 tgctcggtca tcaggcgcgg atcctgaccg acaacgtgca ccggaaagcc cgaattcgca
      661 ccatcgatgg ccctgggctt cgttcctcgc tcgagaccgg aaacgtcctc gtcgtcgcag
      721 ggtttcaggg caccgatgct tacggcaaca tcaccacgct ggggcgcggc ggctccgata
      781 cttcagcggt ggccatcgcg gcggcggtca acgcggacat ctgtgagatc tgcacggacg
      841 tcgacggcgt ctacacgact gatcccggca tctgcccgaa cgcccgcaag ctccagcgga
      901 tcagctatga ggagatgctc gagctttcct cgcaaggggc caaagtcctt cagatacgtt
      961 cggtggagct cgcgatgaag caccggatgc cattgcatgt gcgctcgagt ttttcgaaag
     1021 aagagggaac gatcgtgacc gacgaagaac acctgctcga aaaagtgatc gtggccggcg
     1081 tcgccgcgga caagaagacc gcaaagctca ccgtccacgc tttggccgat catcctggcg
     1141 tggtcgcgag cgtcttcgag ccgctcgcag aagcgaacat ctccgtggac atgatcatcc
     1201 agaacgtgtc gaaagctgga cacacggatc tgacattcac ggtgagccat gaggatgccg
     1261 atcgagccga agccattgcg aaagagatcg tcgcaagcgt tggcgccgag gatgtgattc
     1321 gtgacgatgc cgtggcgaag gtatcgattg tcggcgttgg gatgcgcaac cacgctggta
     1381 tcgctgcgcg catgtttcgg ctcctcgctg acaacggcat caacatccaa gcgatcagca
     1441 ccagcgagat caagacctcc tgcctgatcg agtccgccca cgcggaccgc gcggttgccg
     1501 tgcttcacga cggcttcgag ctcggcaatc cgcaacgctg acttgcgccg accgatggca
     1561 ggcggtgaac cgtgaaaaag gtctttccac aacccgagcc gccgcgagcg tcgtgtttgc
     1621 gctcgcctgc agcgccgcag catcgatcca accgctaccc gtctggttcc gacctcggaa
     1681 tgttcagtcg aaggagacgc taatggactt cgaatcgaac accgaggcgc aacgccgggg
     1741 tgtcgatcgc ggtgttcgcc acgcaaccga ctccgagccc gagcccgagc gtcgcctggt
     1801 cttcgtatac ggaggccccg gactgaagtg actcgttgag tgcgaggcta cgttgactgg
     1861 gcaggggccc cgtgtatagt gccgacgcta tttgccgggt tttcccggtc ttttggagga
     1921 agccttggcc aacgaccctg ccgaaaacga aaaacaagag acgctcaccg cgggccagcg
     1981 actcgcggca cagcaagcgg ccaaggcggc gcgcaaggcc gcacagaaag gacgcgacgc
     2041 agagctcgtc gaagagaagg cgctcgcgca ggccgcggtt gcgaaggatt ggctccaaga
     2101 caacttgaag cctctcggcc tactcgcggg cggcgtgctc ttgatggcag cgatcggaat
     2161 cggctggtcc acattcaacc ggggtcagca aagggccgct ggcgaggagc ttgccgctgt
     2221 cctcgatggt ggcgccgacg acaacgcggt gctcgcgacc gcctacgcca cggttgctga
     2281 cgcacatccc aaaacgcctg cggccgcatg ggcgcgaatc ggcgagggca gggcgctcta
     2341 cgcgagcgga gagtgggagc gcgcgcgcgg ggcctatcag gctgcgctcg aatcgagcga
     2401 cgacgaaacc gtgcgctggg ctgcgctcga aggtatcgcg tacgcgttcg aggccgagga
     2461 atcctacgaa cgggcgatcg aggagctcga ggcgcttcgt gagctcgacc gcaacgtcgc
     2521 cccgatcgct ggctatcatc aggggcggat cctcgcgaag cagggcaagc tcgaagaggc
     2581 gaaatcgaag tttgaccgcg ttctcaacga gctgaaggaa gcggacgcgc ctctgctgcc
     2641 gtacacgcaa gagcaaactg aggcgcggct tgccctgatc gacccgagtg tcgcaccggc
     2701 cggagggatc gatccccgaa aggcagacga gttcattcgg cagatgaacc aaatgctcca
     2761 acgccagcca cctgcggagt gagcacgcga gcgatgcgca tccgctttgg ctctgctcgg
     2821 actgctgccg cctgggtact cgttgcttcg ttcgcgctca tggcgatcgc cggctgcggc
     2881 gccgctggta cgccgacgag caatgccgac aatttcggat gggtgtcgca gggaaatgac
     2941 tacgcccctc caggcgaggg tggcttcaac gtccgctggt ccaagcagct caccgatcgc
     3001 tgggaggcac gctatctacc ggtcgagctc agctcggcgg aattcgatac gaagcgtgag
     3061 cgtgtctaca taggcactac ggaaggaaac ttctttgcct tcgagatgaa cgggaggcgc
     3121 ctgttcttct acgacgcaca cgcccagatc gagtcaaagg ctggggtgga cgcggacacg
     3181 ggcgaggtat tcttcggctc ggtcgacggc accgttcacg ccctcagtgt gaccggcgag
     3241 ctcaaatgga aggccaagct gatcggttcg attcgagcac agcccgtgct tgccgcgggt
     3301 gcgctctacg tcgtgaccga caccgacacc gtcaccgcgc tcgcacggga cgacggtcgc
     3361 gtcctgtgga cctacgacaa ggaaccggtc gaggagatca ccatcgcggg acacgcgggg
     3421 atgctgctcg aagacggccg cttgtatgca gccttcaccg atggtgccgt ggccgcgctc
     3481 aacccggcgg acggaagcct ctactgggag ctcgaaaccg cgctcgatgt cgagcttcga
     3541 ccaggcaacg ttccgcagtt cctcgatgtc gacacgactc cggtcgtgcg ccaagggaca
     3601 ctctacgtcg cctcgttcac cgcaggcctc tacgcgctga atgcctccag cggcagcgtc
     3661 gagtggcggg acgcggactt caagggggtg accgggctcg cgagtgtcgg gcgcatgctg
     3721 atcatcacgt ctgcaccgcg gggcgtctcg cttctcgact tgagaacgcg ggaaatccaa
     3781 tgggagaagg cgccggagcg aggtgcgcct acgccgccgg tcgtgacgga gacgggtacg
     3841 gtcatctacg gcgaaaccca gggctcgctc ctggcactct cgctttcgga cggccatgaa
     3901 atcgcaagag cggaaggggg ctccggcttt tccgcgacgc ccgctgccgc gggtgctttc
     3961 ggtggagcga tctcgaacgg cggccgcttc ctctgtctcc ggatcaacta ggcgctcgaa
     4021 ccgtcaatcg ccgttacccg cgaccagcgg atacacgacg ccaccgatcg cgccgccgat
     4081 gatcggcgcc acccagaaca accacagttg ctgcagcgcc cagggctctg cgaaaatggc
     4141 gaccgccgtg ctccgtgcgg ggttcaccga gaggttgtcg accggaattc cgatcaagtg
     4201 gatcagcgta aggcagaggc caattgcgac cggggcaagc gctgcgggcg cgcgcttgtc
     4261 tgtcgcgccc aggatgacga agagaaacgc gaacgtgaga acgatttccg ccgtgagtgc
     4321 cgcggccatc gaatagccac ctggcgagtg ctcagcgaaa ccgttcgagg caaagccgcc
     4381 ggcctggaac ccgtcctggc ccgaagcgat gaagtagagg accgccgcgc cggcaatgcc
     4441 accgagcacc tgcgcgatca catacgggag caactccgaa gccgggaatc gcttgccgag
     4501 gagaaggccc actgaaaccg ccggattgag atgacagccc gaaatgtgtc cgatggcata
     4561 ggccatcgtg aggaccgtga gtccgaacgc gagggccact ccaaggaacc cgattccaag
     4621 ctcgggaaat cccgcggcca gtaccgcgct cccacagcca ccgaacacca gccaaaatgt
     4681 gcctacaaac tccgcggcaa ccttgtttcc tagcgacatg caacctcctt cgcccggatg
     4741 ccccgggccg gggcgcatgg tcgcactgcg gtgcacccct tgtaaacgag tgcatcatgt
     4801 caaaaccaga cgttggtgtc cggtcgttct gccagttaga aatgtccggc accgaatggg
     4861 gaccatgggg cagggagcct gcagcgggca gggcggcgag cggcctggag aacctggtga
     4921 atcgggggga acgtcgctgt cgggcaagag ggcggatgtc ttgagaggtc gtcggtaggc
     4981 atcgagcagg gccgccgttt caaggtcccg gcggcggcaa ccggtcggca aagcggtcgc
     5041 ggacgatgcg cttttcacgc ttgctcttgg cagaccggac gagctcctgc tcgcgttgct
     5101 gttggcgctt tcggatttcg gtgagggcgc cacttaggag cttgttgtcg acaatggagg
     5161 cttgggtcac gcagccatcc ttgtgaaacg gtcgagccgc aagcgggaca cctcgatatc
     5221 gaatcgacac gccgccgtcc tcgtactcga cgacggtgac ctgtttgcgc gccgcggctt
     5281 tggcctcttc ggtaggctcg agcagataca tcgtgcgctt gtgatgcaca gtgagctgcg
     5341 cggtcacgcg ccgctcttcc tgccaggtga agatgtcgtc gagacagtcg cgctcgagca
     5401 agggccgatg cacatcgtgg tcgtttcggg gcgcccgtgc gaagcgccga ttgtagtcct
     5461 cgatgaacgc gggcagatac cgattggcgt cttgcatcgt agagatgcgg cgcagccgga
     5521 gctccttgac gagccggtct tgaagcgttt ggtgtgcgcg ctcgacccgg cctttggccg
     5581 ccggcgtatt ggcgcaaatc acgtcgatat tcagctcggt catcgcgcga ccaaactgcg
     5641 tgaagccgtc gcccgcttcc gggctcttgg cattgacgcg gaacaccccg gctttgtcgc
     5701 tatacagcgc gaccggcttg ccgtgacgct gaagataggc gcgggtcgcg tggaagtagc
     5761 tgaacgccga ctcggtttcg ctcatccgca gctccatcag cttgccggtc gcgtcgtcga
     5821 cgtagaccaa gagcacacaa ccgggtccgc ggtcttcgaa ccagtggtgg tcgcac
//
LOCUS       DASRZF010000128         2698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3871097, whole genome shotgun sequence.
ACCESSION   DASRZF010000128 DASRZF010000000
VERSION     DASRZF010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3871097"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..1331)
                     /locus_tag="VFG22_05925"
     CDS             complement(39..1331)
                     /locus_tag="VFG22_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415812.1"
                     /translation="MGRLVVLIWVLFGAVGAMAQATEVAPAQPGPTWPLVKSVIGLSI
                     LLILAWLASHPKVRRRLEDALGIGHAITSGLAFVLLGLLARHPAVGILDDEVLYDLTP
                     LVHFALGWLGLIIGFQFDVRVLDRLPRGTATLVGLETLTPFLLVGTTGAALMLFFGRP
                     WNDATFVRDAIVLATAGAISATGPSERVAPQGAFGGEPDPMRAVESLDEIIAVVGLAA
                     LGAFFRPLWIEFEWDLPGTVWLILSLGVGLCLGLIVYALLAGRRGSEFLALLLGSVAL
                     ASGIAGYLLLSPLVLCFIAGALLVNLPGEHKQLCWKILTQLERPIYYLFCAIVGALWH
                     VADWRGWVLLIAFLSSRGIGYAIAKRMLGRQSPRLAARVEASGWRLTLFPHISMVSIA
                     IVMSAQVLYRDPAIPWIVTAVIGGAIASELISRRRRSR"
     gene            complement(1460..2206)
                     /locus_tag="VFG22_05930"
     CDS             complement(1460..2206)
                     /locus_tag="VFG22_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold domain-containing protein"
                     /protein_id="HET6415813.1"
                     /translation="MPQLIAAIAPHHPTATHSERVADRFCGPPGSGNGGYTIGMLGKY
                     VGPEVEVTLKRPIPLDEGLQVQVEAEGGATLGHGSTEIASARHAELDIDIPEAVSFSR
                     ASDARMSYPGFQTHPFPGCFVCGTSRCAGDGMCLFTGPVGEGLVASSWVPSGEFASKE
                     GLIAPELICSALDCPGAWALIERYGIEGPVVLGRMTYRFERPIRSDQRYVVMGWAIGR
                     EGRKTFCGTGVYDADGKVCAVAGATWIQLS"
     gene            complement(2285..>2698)
                     /locus_tag="VFG22_05935"
     CDS             complement(2285..>2698)
                     /locus_tag="VFG22_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415814.1"
                     /translation="DEENPFLSTALVGHTAGAIDDDAWRALVGSFRGRAVPGKLWAYF
                     DGPVRAMRCGAAFAQQDPVSFGVHTGEVVRSGVTVSGSAFSVAESVAERAPLGEVWAT
                     QVLVDLVPGSDLQLDETKEVLQWQGREIALFSVRR"
BASE COUNT          578 a          865 c          824 g          431 t
ORIGIN      
        1 tacgagcgac ccaagcgcca aaaggatgag caggttcttc atctcgaacg tctccggcga
       61 gaaatcagct ccgaggcgat cgcacccccg atgacggcag tcacgatcca gggaatggcg
      121 gggtctcgat agagaacttg cgcgctcatc acgatcgcga tggaaaccat gctgatgtgt
      181 ggaaatagcg tcaaacgcca gcccgacgcc tcgacgcggg ccgccaaacg tggggactga
      241 cgaccgagca tgcgctttgc aatcgcgtat ccgattcctc gactcgagag aaacgcgatc
      301 agcagcaccc agccgcgcca atcggcaacg tgccaaagcg ctcccacaat cgcgcagaag
      361 agatagtaaa tggggcgctc gagctgcgtg aggatcttcc agcacagttg cttgtgctcg
      421 cctggcaggt tcaccaagag ggcgcctgcg atgaagcaca gcaccagtgg ggagagtaac
      481 aggtatcctg cgatcccgga agccagtgca accgaaccga gcagcagcgc caagaactcc
      541 gaacctcttc gccctgctag cagtgcgtac acgatgagac ccaagcacag tccaacgcca
      601 aggctcagaa tcaaccatac cgtccccggc aagtcccact cgaactcgat ccacagaggg
      661 cggaaaaagg cgccgagcgc tgcgaggcca acaaccgcga tgatctcgtc gagtgactcg
      721 acggcacgca tgggatccgg ctccccaccg aacgcgcctt gcggtgccac ccgctcgctc
      781 ggtccggttg cagatatcgc tcctgccgtc gccagcacga tcgcgtcacg gacgaaggtc
      841 gcgtcgttcc aaggcctgcc aaagaagagc atcaacgcag cacccgtggt gcctacgagc
      901 agaaatggcg tcagcgtctc caggccgacc agagtggccg tgccgcgtgg gagccgatcg
      961 agaactcgga catcgaactg aaatccaatg atgagaccga gccagcccag tgcgaagtgc
     1021 acgagaggtg tcagatcgta gagcacctcg tcgtcgagga ttccgacggc aggatgacgc
     1081 gccagcaatc cgagaaggac gaaagcaagg cccgaggtga tcgcatggcc aatgccgagt
     1141 gcatcctcga gccgccgccg gaccttcggg tgtgatgcca accaagcgag gatgaggagt
     1201 atcgaaagac cgatgaccga cttgaccagc ggccaggtgg ggccgggttg agctggagcc
     1261 acctccgtcg cctgtgccat ggcaccgacc gcgccgaaga gcacccaaat caacacgacc
     1321 aggcgaccca tcggcggact gtagccgaga ataggacgtc ggtgtgtttc cgaggcgcac
     1381 tctcagataa ccgatgagga cttgtggacc gtcttcgcgt ttctgaagac gctcccgccg
     1441 atcgccaacg tggttccgct cagctgagct gaatccaggt ggccccggcg acggcgcaga
     1501 ccttgccgtc ggcgtcgtac acgccggtac cgcagaacgt cttgcggcct tcacggccga
     1561 ttgcccagcc cattacgaca tagcgttggt cggaacggat tggtcgctcg aatcgatagg
     1621 tcatgcgtcc gaggaccacg ggcccttcga tgccgtagcg ttcgatcagc gcccaggcgc
     1681 cggggcagtc gagtgcagag cagatgagct caggcgcgat caggccttct ttgctcgcga
     1741 actctccgga tggaacccag gagctggcca cgagaccctc gccgacggga ccggtaaaga
     1801 gacacatccc gtctcccgcg cagcgactcg tcccgcagac gaagcaaccc gggaaaggat
     1861 gtgtctggaa gccggggtag ctcatccgag catcggatgc gcgcgaaaaa ctcacggcct
     1921 cggggatgtc gatatcgagc tccgcatgcc gtgcgcttgc gatctccgtg ctgccgtggc
     1981 cgagcgtcgc gccgccctct gcctcaacct gcacttgcaa gccctcatcg agtgggatgg
     2041 gacgcttgag cgtcacctcg acttcggggc cgacgtactt tccaagcatg ccgatcgtgt
     2101 atccgccatt tccggatcct ggcggaccgc agaaacggtc ggcgacccgt tcgctgtgtg
     2161 tggcagtggg atgatggggt gcgattgcgg cgatgagctg tggcatgggg acctccttga
     2221 tgagcaccaa ggtacggagg cgggctgcga aggtcctttg cgcagcctta ccaattgctt
     2281 agctctagcg ccggacagaa aacagcgcga tctcgcgccc ttgccattga aggacctcct
     2341 tggtttcatc gagctggagg tcgctgcccg gcaccagatc gacgagcacc tgagtcgccc
     2401 agacctcccc gagcggcgct cgttctgcaa ccgattcggc aactgaaaag gccgagcctg
     2461 agacggtcac cccgctacgg acgacttccc ccgtgtgcac accaaacgac accggatcct
     2521 gttgagcgaa agccgctccg cagcgcatgg cccgaaccgg gccgtcgaag tacgcccaga
     2581 gcttccccgg cacggctctg ccgcgaaagg acccgaccag cgcgcgccac gcgtcgtcgt
     2641 cgattgcacc ggccgtatga cccacgagag cagtgctcaa gaatggattt tcttcgtc
//
LOCUS       DASRZF010000129         3173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2837998, whole genome shotgun sequence.
ACCESSION   DASRZF010000129 DASRZF010000000
VERSION     DASRZF010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2837998"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..693)
                     /locus_tag="VFG22_05940"
     CDS             complement(49..693)
                     /locus_tag="VFG22_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415815.1"
                     /translation="MSPADAREALIREALRLHERYHRELIERFSVCPWAKAARVEGRT
                     RTHVVAEVSCSAAELTPLVRAWAADETVDVAFVIVPRFTSGRDAFAEWVSSLAEQTND
                     VFFSAPFYPGAPDETGSIQFLRQTPDPTAQLVRRSRLEVIRAQDPPHYRDIFELDLRN
                     LEVAKAPSTVAASVHAYNQRTLEREGRAGLKAILEDIRNDRNQTYALLDEMRFD"
     gene            complement(690..1160)
                     /locus_tag="VFG22_05945"
     CDS             complement(690..1160)
                     /locus_tag="VFG22_05945"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET6415816.1"
                     /translation="MINALIRAVYRVAHWGLRLLWFIRRPETTGALVAVWYQGRVLLV
                     KNSYRAQLTLPGGYIRPREERRVAAARELREEVGITVQPKRLVHAYHGTHLFEHRKDT
                     LDIYELEVEDEPKVLVDEREVVRAEFHTPDEALNMQIVPHLEEYLARRLGQDAR"
     gene            complement(1218..2597)
                     /locus_tag="VFG22_05950"
     CDS             complement(1218..2597)
                     /locus_tag="VFG22_05950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET6415817.1"
                     /translation="MKAASGPSKGVDRTIFRRLFDLAMPIVGLNLLSVLTLAIDTAMC
                     GRLVDAEEALTALGFASQLIFLLMVAMLGLTVGTVAFIARAHGAGDSSRVNHVLHQSV
                     LLTYLVSVAVALVGNLGAGFAMDMLGADATVRNLGVDYLRPLFSLTVFFYLNMLFAAA
                     LRAVGNTMLPFMIALFSNFLNIVLNYGLILGRLGFPAMGVRGAAWGTVISQAVAVFLM
                     VVILRRDVVQGVRARLHLAPLDRPLANDLFRVGAPAALDMVILNVAFLSIVGMLARVA
                     QVTVAAHGIGLRIQSLAFVPGLGVSQAIGAMVGNALGAGSVPEAREVVRGGVILSTVI
                     MTVIGLGIVLGAEPIIAIFDVEPATELGRYSMLWMRVLGWGMPVVGVHIALIGMLRGA
                     GATNTSLAINVVGTVIVQVPLSWFLGFVVGWGAFGIWVALPISFVVRMALCILAYRRG
                     SWARAGETI"
     gene            2671..>3173
                     /locus_tag="VFG22_05955"
     CDS             2671..>3173
                     /locus_tag="VFG22_05955"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6415818.1"
                     /translation="MAGEPRDFGPYRLVRRLGVGGMAETFEATRLGAGGFEQRVCLKR
                     VLPAFCGDEEFVTRFRREAKLAAQLRHTNIVGVIDFGEIDSVQYMSLELVDGLDLRSL
                     LKSSPRERLPADWVALIGTDLACALEHAHESLVHRDISPSNILLSRSGEAKLADFGIA
                     KALEDTAA"
BASE COUNT          632 a          985 c         1024 g          532 t
ORIGIN      
        1 cggagcagcc tggggactcg tctcgtccgg ctacatccaa cgcaagcttt agtcgaaacg
       61 catttcgtcc aagagcgcgt aagtttggtt ccggtcgttt cggatgtctt ccaaaatcgc
      121 tttgagcccg gctcttcctt ctcgttccag cgtgcgttga ttgtaagcgt ggaccgaagc
      181 ggcgacggtg ctgggtgcct tcgcgacttc gaggttccga aggtcgagct cgaagatgtc
      241 tctgtagtgg ggtgggtcct gggcgcggat cacttcgagg cggcttcgtc gaaccagctg
      301 ggccgtcggg tcgggggtct gacgcaggaa ctgaatcgag cctgtttcgt caggagcgcc
      361 cgggtagaac ggcgcgctga aaaacacgtc gttcgtctgc tcggcgagcg agctcaccca
      421 ttccgcgaag gcatcgcgcc cgcttgtgaa tcgggggacg atcacgaatg ccacgtcgac
      481 cgtttcgtcc gcggcccagg ctctcaccaa gggcgtcagc tctgcggcgc tgcacgaaac
      541 ttcggcgacg acgtgtgtgc gtgtgcgacc ctccacgcgc gcagccttgg cccaggggca
      601 gaccgagaag cgttcgatca gctcccgatg gtagcgttca tgaagtcgga gcgcctcacg
      661 tatcagcgct tcacgggcgt cggcagggct caccgagcgt cctgcccgag ccggcgcgcg
      721 agatactctt cgagatgtgg gacgatttgc atgttgagcg cctcgtcggg cgtatggaac
      781 tcggcccgaa cgacctcgcg ctcgtcgacc aggaccttgg gttcgtcctc gacttccagc
      841 tcgtagatgt cgagggtgtc ttttcggtgc tcgaagagat gggtgccgtg atacgcgtgc
      901 accaatcgct tgggttgaac agtgatgccc acttcctcgc gtagctcgcg tgctgcggcc
      961 acccgccgct cctcgcgcgg acggatgtag ccgcccggca aagtgagctg cgctcgatag
     1021 gagttcttca cgagtaagac tcggccctgg taccagaccg ccacgagcgc ccccgtcgtc
     1081 tctggtcgcc gaatgaacca gagaagccgt agcccccaat gcgcgactcg atacaccgcc
     1141 cggatcaagg cgttgatcat tccccgaggt taccaaaaga cgccactcag gagaaatccc
     1201 tgctcaggga gtggtgctca gatggtctcg cccgcccggg cccagctgcc acggcgatac
     1261 gcgaggatgc acagggccat ccgcacgacg aacgaaatag gcagtgcgac ccagattccg
     1321 aaggcgcccc aaccgaccac aaagccgagg aaccaactga gcggcacctg aacgatcacg
     1381 gtcccgacga cgttgatcgc caaactggtg ttcgtggcgc cggccccgcg aagcattccg
     1441 atcagcgcga tgtgaacgcc cacgaccggc atgccccagc cgaggacacg catccaaagc
     1501 atcgaatagc gaccgagctc cgtggctggc tccacatcga agatggcgat gatcggctcc
     1561 gcgccgagga cgatcccgag accgatgacg gtcatgatca ccgtggagag aatcaccccg
     1621 ccgcgcacga cctcgcgtgc ctcgggtacc gagccggcgc cgagcgcgtt gccgaccatc
     1681 gcgccgatgg cctgggatac gccgagtccc gggacgaagg ccagcgactg gatcctcaat
     1741 ccgattccat gtgccgcgac agtgacctgc gccacccggg cgagcatccc tacgatcgac
     1801 aggaacgcga cgttgagaat gaccatgtcg agcgctgctg gcgcacccac ccgaaagagg
     1861 tcgttagcga gcggacgatc gaggggtgcc aagtgcaggc gggcccgcac accttggacg
     1921 acgtcacggc gaaggatgac gaccatcaag aacaccgcga cggcttgcga gatcacggtc
     1981 ccccaggcgg cgccgcgaac gcccatggcg ggaaagccga gtcgccccaa gatcagcccg
     2041 tagttgagca cgatgttgag gaagttgctg aagagcgcga tcatgaaagg aagcatggtg
     2101 tttccaaccg cgcgcaaagc ggctgcaaag agcatgttca gatagaagaa gaccgtcagc
     2161 gaaaagaggg gccgcaggta gtcgacaccg aggttacgga cggtcgcatc ggcgccgagc
     2221 atatccatcg cgaagccggc acctaggttt ccgacgagtg ccacggcaac cgagacgaga
     2281 taggtgagga gaacgctctg gtggagcacg tggttgacgc gcgagctgtc tccggcgcca
     2341 tgtgcccgcg cgatgaatgc gacggtgccc acggtcaacc cgagcatggc caccatgagc
     2401 aagaagatca gctggcttgc gaaaccgagc gccgtcaacg cctcctcagc gtcgacgagc
     2461 cggccgcaca tcgcggtgtc gatggcgagg gtgagaacgc tcagcaagtt caaacccacg
     2521 atcggcatcg ccaaatcgaa gaggcgccgg aagatggtgc gatcgacgcc tttggatgga
     2581 cctgacgctg ccttcatcag tggccgggga gcgtgcatcg acagccctgc tggcgccagc
     2641 gctttcccga tggtgctagg ctgagcatgg atggcgggcg agccgcgaga ctttggtcct
     2701 taccgtttgg tgcgccgact cggcgtgggc ggcatggcgg aaacctttga agcgacgcgc
     2761 ttgggcgcag gtggcttcga gcagcgtgtc tgcctcaaga gagtcctgcc cgcgttctgc
     2821 ggagacgaag agttcgtcac tcgctttcgc cgcgaggcca aactggcagc gcagcttcga
     2881 cacaccaaca tcgtcggcgt catagacttt ggggagatcg acagcgttca gtacatgagc
     2941 ctcgagctgg tggacggtct cgacctacga tcgctgctca agtcatcgcc aagggaaagg
     3001 ctcccggccg attgggtggc actgatcggg accgacttgg cgtgcgcgct cgaacacgca
     3061 cacgaaagcc tggtgcaccg ggacatctcg ccgtcgaaca tcttgctcag tcgctccggt
     3121 gaagcgaagc tcgcggactt cggcatcgcg aaggctctcg aggacacggc ggc
//
LOCUS       DASRZF010000130         4123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_431770, whole genome shotgun sequence.
ACCESSION   DASRZF010000130 DASRZF010000000
VERSION     DASRZF010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431770"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VFG22_05960"
     CDS             complement(<1..401)
                     /locus_tag="VFG22_05960"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HET6415819.1"
                     /translation="MPGEPLIAVFVDFENLAIGAQVTESGRFQIELVLKRLLEKGRIV
                     YKRAYCDWSRYRNAVREFHGQGIGLVDIPQTRMSGKNSADIHMVVDALDLCYSKDHID
                     TFALLSGDSDFSPLVYKLKENDKRIIGVGVK"
     gene            623..1405
                     /locus_tag="VFG22_05965"
     CDS             623..1405
                     /locus_tag="VFG22_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415820.1"
                     /translation="MLPRISKVDQDFWNIRGSFKIGGVVEIGTQTSLVRRASGKFVFL
                     DSCGLNESVENEVLALTDGGESVEAILNLHPFHTVYAKKLHERFPKARLYGTARHVSR
                     FPELRWEPQRTEDSALHGMFAEDFEFSVPRGVDFISSNENVHFSSVLVFHRSSKTIHV
                     DDTLMYVRFPKPLRMLGVTDLLSFHPTLSQALERRAGAARDFRGWAEELAERWRDSEN
                     LCAAHVAALMAKANSGSSIHARILQALDKAESKLTKHERKYG"
     gene            complement(1442..2461)
                     /locus_tag="VFG22_05970"
     CDS             complement(1442..2461)
                     /locus_tag="VFG22_05970"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HET6415821.1"
                     /translation="MANVSVVGAGAFGTALALYSLKLGHQVRVWAFDEGLPERVAAEG
                     ENAMYLPGFPVPKEMVFTNDLAEALDGAELVLLGIPSAYMRSVTAQVAPHLPPGALVT
                     STAKGVERGSLALVSDVLAETLPDHVASSTYLSGPSFAKDIASGFPAQVSLAAFDIDT
                     ARRVQSILHSPRLRVYASDDVIGVQLGGALKNVIAVACGAADGLGMGASAVASLMTRG
                     LAELARLGVALGANPLTFLGLAGVGDLTLTCTGELSRNRQLGKKLASGKTAKEILASQ
                     KAVAEGYYTAAAVHTLAAREDVEMPVSEAVYRVCYEDVPVLEEATRLMMREPKDELEG
                     IFLRK"
     gene            complement(2644..>4123)
                     /locus_tag="VFG22_05975"
     CDS             complement(2644..>4123)
                     /locus_tag="VFG22_05975"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET6415822.1"
                     /translation="AKPISTGFHTAAAASNDDTIILDLKRMNRIVDIDEKNQIAVIEP
                     YVRAIDLQTELLKIGLNCHIISAGAMHSLLASHTAAWGYGVTGASTSFSGRNLLGVEW
                     VLPSGEIVTLGSAGSGGGWFSCEGPGPSMRGVMRGFHGTFGGLGVFTKCSIKLYKYDG
                     PRAWDFEGQSPNYVLKEIPERSQFTAIAFPSRDAEKDGGYKMGEAELNYGEFRTPMFF
                     TALGLTNNNLELKEALDSGLFPKFANHVLSCAFFAASDGEFAWKQKALAEILKETNAA
                     PVPLNLSASVRSLKVAGKLLAGVKDPLAPIRKSTRLQKFAASSSFGADQRRATQSRLF
                     WLLVRNAVNIQSAFRPSQGMATMMGAFDTWDLAHTQAAYAAKAKQKYIEQGLIIADGG
                     DLGAGGTFENGHLGYLEGIILYDPSNPASVEAVRDLVEGGLRASIDQAMGVPIAGFGV
                     NANKELGPACYHYDHWVARLKAALDPNTASDPFFYSEPETDD"
BASE COUNT          793 a         1240 c         1268 g          822 t
ORIGIN      
        1 ttcttgactc cgactccgat gatgcgcttg tcgttctcct tgagcttgta aacgagtggt
       61 gaaaagtcgc tgtctcccga gagcagggcg aacgtgtcga tgtggtcctt cgagtaacag
      121 aggtccagcg catcgaccac catgtggata tccgcactgt tcttgccact catgcgggtc
      181 tggggaatgt cgaccaaacc gatcccttgc ccgtggaact cccgtaccgc gtttcgatag
      241 cggctccagt cgcagtaggc gcgcttatag acgatgcggc ccttttccaa caagcgcttg
      301 agcacgagct cgatttggaa acggcccgac tccgtgacct gggcgccaat ggccaagttc
      361 tcgaaatcga cgaagacagc gatcagcggc tcaccaggca tacactcctc gaacggctcg
      421 caggataggc gtcgtggccg atgtacgcca ccctgcattc aggcgccggg tcgaacgacg
      481 ttcgaactgg ccttcaatcc gacggcgcgc cgccgagcag catcccgttt cttgctgttg
      541 aattccgcct caagcgcagt gtagctgcaa agccacgttc tgatcgctga caaaccgctg
      601 aggtggcgta agatgagtcg ccatgctccc tcggatttcg aaagtcgacc aagatttctg
      661 gaacattcga ggatcattca agatcggcgg cgtcgtcgag attggtacgc aaacctcgct
      721 cgtcaggcgt gcgagcggta aattcgtctt tctcgattcc tgtggattga atgaatccgt
      781 cgagaacgag gtgctggccc tgaccgacgg gggcgagagc gtcgaagcga ttttgaactt
      841 gcaccccttc cacaccgtct acgcgaagaa gctacacgag cggttcccga aggcccgact
      901 ctatggcact gcgcggcatg tgtcgcgatt cccggaactg cgatgggaac cgcaacggac
      961 ggaggattcc gcattgcacg gcatgttcgc ggaagacttc gagttctccg tgccgcgtgg
     1021 cgttgatttc atctcctcga acgagaacgt tcatttttca tcggtcttgg tctttcaccg
     1081 ctcatcgaag acgattcatg tggatgacac cttgatgtac gttcgttttc cgaagccgct
     1141 gcgaatgctg ggtgtcaccg atttgctgag ctttcacccg accctttcgc aagcgctcga
     1201 gaggcgagca ggcgcggctc gcgatttccg cggctgggcc gaggagctcg cggaacgctg
     1261 gcgtgattcc gagaatctat gtgcggcgca tgtggcggcc ctcatggcaa aagccaattc
     1321 agggtcgtca atccacgctc gcattcttca ggcgctcgac aaggccgaga gcaaactgac
     1381 gaagcacgaa cgcaagtacg ggtgaggcag gccgatgcca ggggctcgtt caccggctcg
     1441 actacttgcg tagaaagatc ccttctagct cgtcttttgg ctcccgcatc atgagccgcg
     1501 tcgcctcttc gagaacggga acgtcctcgt agcagacacg gtaaaccgcc tcggaaacgg
     1561 gcatttcgac gtcttcccgg gcggcgagag tgtgaaccgc ggcggcggtg tagtaaccct
     1621 ccgcgaccgc cttctgtgag gccaggatct ctttggctgt ctttcccgag gcaagcttct
     1681 tgccgagctg gcggtttcgg gagagctctc cggtgcaggt gagggtgagg tcgccgactc
     1741 cggcgaggcc gaggaaggtc agcggattgg cgcccagggc cacgccgagc cgcgcgagct
     1801 ctgcgaggcc gcgggtcatc aaggaggcaa cggcggatgc tcccatgcca agaccgtccg
     1861 cggcgccgca tgccaccgcg atcacgttct tgagcgcgcc accgagctga acgccgatga
     1921 cgtcgtcgct cgcgtacacc cggagccgag gtgaatgaag gatcgattga acgcggcgcg
     1981 cggtgtcgat gtcgaacgct gcgagactga cctgggccgg aaagcccgac gcgatgtcct
     2041 ttgcgaagct cggaccagag agataggtcg agcttgcgac gtggtccggc agcgtctcgg
     2101 ccaagacgtc gctcaccagt gccaagcttc cgcgctcgac gccctttgcg gtcgaggtga
     2161 ccaaggctcc tggtggaagg tgcggggcaa cctgcgcggt gacggagcgc atgtacgcgg
     2221 aggggatgcc gagcagcacc agctctgcac cgtcgagcgc ctcggcgaga tcgttggtga
     2281 agaccatctc cttcggcacg ggaaaccccg ggaggtacat ggcattctcg ccctcggcag
     2341 cgacccgctc tgggaggcct tcatcaaagg cccacacacg cacctggtga ccgagcttca
     2401 aactgtaaag ggccagggca gtgccaaacg cccctgctcc aaccacactg acgtttgcca
     2461 ccgctcgcct ccactgcatg ggcgccgcac gacgtgcacg agctcccgag cggggaatca
     2521 tacaggtgga cgcgttctag tttcaaccgt ggattcacgg gctcttcggg tgaccgcgtc
     2581 gtttgcgacc tctcagggcc tgaaagcggc ggcgtatttc caaacgcatc gcgtatcagg
     2641 ggctcagtcg tcggtttcgg gttcggagta gaagaacggg tcggatgccg tgtttggatc
     2701 gagagccgcc ttgaggcgcg ccacccagtg gtcgtagtgg tagcaggcgg ggccgagctc
     2761 cttgttcgcg ttgacaccaa atcccgcgat cggtacgccc atggcttggt cgatcgatgc
     2821 gcgaagaccg ccttcgacca gatcgcgcac cgcctcgacg gaagccgggt tgcttgggtc
     2881 gtatagaatg atgccctcga gatagcccag atgaccgttc tcgaaggttc cgccggctcc
     2941 gaggtcacct ccatccgcga tgatcagacc ctgctcgatg tacttctgct tcgccttcgc
     3001 tgcgtacgcg gcctgggtat gagcaagatc ccaggtatcg aatgcgccca tcatggtagc
     3061 catcccctga gacggccgga atgcggactg gatgttgacc gcgttgcgca cgagcaacca
     3121 aaacagccgc gactgggtcg ctctgcgttg atcggcgccg aagctcgacg atgcggcgaa
     3181 tttctgaagt cgcgtggatt ttcgaatcgg cgcgagcgga tctttcaccc ctgctagcaa
     3241 cttccccgcg actttgagcg agcgaaccga ggcagaaaga tttaagggta ctggcgcggc
     3301 attggtttcc ttcaagatct ccgccagcgc cttttgcttc caagcgaact cgccgtcgga
     3361 ggcagcgaag aacgcgcaag acaagacatg gttcgcaaat ttcgggaaca ggccgctgtc
     3421 gagcgcttcc ttgagctcga ggttgttgtt ggtgagtccg agggcggtga agaacatcgg
     3481 ggtccggaac tcaccatagt tgagctctgc ctctcccatc ttgtagccac cgtccttctc
     3541 tgcatcgcgg gatggaaagg cgatggcggt gaactggctt cgctcgggga tctccttgag
     3601 tacgtagttc ggggactggc cctcgaagtc ccaagcccgt gggccgtcgt atttgtaaag
     3661 cttgatcgag cacttcgtga acacaccgag gccgccgaag gtcccatgaa agccacgcat
     3721 cacgccgcgc atcgacgggc cagggccctc acagctgaac catccaccgc ccgagcccgc
     3781 gctccccaag gtcacgattt ccccgcttgg aagcacccat tccacaccga gcaggttgcg
     3841 gcccgagaaa ctcgtcgaag cgccggtcac gccgtacccc caggctgcag tgtgggaagc
     3901 cagcagggag tgcatcgcac ccgcggatat gatgtggcaa ttgagcccga tcttgaggag
     3961 ctccgtctgc aagtcgatcg cacgcacata gggttcgatg actgcgatct gattcttttc
     4021 gtcgatgtcg acgatccgat tcatgcgctt gaggtcgagg atgattgtat cgtcgttcga
     4081 tgcagccgcc gcggtgtgga atcccgtgga gatcggtttg gcc
//
LOCUS       DASRZF010000131        17811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1117636, whole genome shotgun sequence.
ACCESSION   DASRZF010000131 DASRZF010000000
VERSION     DASRZF010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 17811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17811
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117636"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..857)
                     /gene="hisIE"
                     /locus_tag="VFG22_05980"
     CDS             complement(195..857)
                     /gene="hisIE"
                     /locus_tag="VFG22_05980"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HET6415823.1"
                     /translation="MSLDALKYDDQGLVAVIAQDAETGEVRMMAYADRAAIERTLATR
                     AAHFFSRSRQQPWKKGESSGNTLGVRSVWVDCDGDTLVYMVDPAGPSCHTGAETCFFR
                     RLNAEGELIEAGEQSAAPTLLRLERTLEARKASDAGKSYTKALLDAGPTKIDAKLREE
                     AAELGQALIGESDERVASEAGDVIYHLLVGLVSRGVSLRALFSELSKRFGQSGHEEKA
                     KR"
     gene            complement(1089..2009)
                     /locus_tag="VFG22_05985"
     CDS             complement(1089..2009)
                     /locus_tag="VFG22_05985"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HET6415824.1"
                     /translation="MVDKVDKIWLDGKLIPWEQANVHVLTHTLHYGLGVFEGIRCYKL
                     EDGRSAVFRLEEHIRRLFDSAHICMLPMPFSQAEIVAACLETVRANRLDECYLRPIAF
                     MGDGEMGLGAKTNATRVSIIAWRWGAYLGEEGLTRGIRAKVSSFQRAGVNTLMSKGKV
                     NGHYVNSILAKREVQAIGYDEAIMLDTQGYVSEASGENIFVVRDGEIATPPMGVSILG
                     GITRNSVMRVMRDLGLIVHERLISRDELYIADEVFMVGTAAEVTPVRELDDRSIGQGS
                     RGPITEKVQSAYFDAVRGKNTPEPSWLSIV"
     gene            2170..2553
                     /gene="yajC"
                     /locus_tag="VFG22_05990"
     CDS             2170..2553
                     /gene="yajC"
                     /locus_tag="VFG22_05990"
                     /inference="COORDINATES: protein motif:HMM:NF014725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HET6415825.1"
                     /translation="MLYSLFHILLQPDVSGGPVTAPTGGGGPAPGPMGCGGGGTEQIG
                     LLLLMFAVLYFLMIRPQQKRAKQHEAMMGALKKGDVVRTNGGILGEITALDERDVTIE
                     VADRTRIKVLRSHIAGLDQTPEQKS"
     gene            2571..4370
                     /gene="secD"
                     /locus_tag="VFG22_05995"
     CDS             2571..4370
                     /gene="secD"
                     /locus_tag="VFG22_05995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HET6415826.1"
                     /translation="MDRGWYFRLAIVVGTSLLGFLALWPSVDRWLPCPDIVKETFENQ
                     INPGLDIKGGLRLMYEVEVDEYIRDRRDRIAEQVQRQCGVLIGIVPEEEVDDIDKATL
                     TAIQQRCKVERVDKDGDAVRAIRITFSNQEDAQKLSKTWVKDYFRDLRVASGVGDLVV
                     DLHMREEQLANMRETAVRQSERTITNRIDNLGVLEPTVIARPNDGDIIIEIPGAQESS
                     FERIKSIISRTAQLQFQIVDDESDSRSVFTAPPEGIYLDADSQTFAEAFGEDARDRLR
                     SYIATLPVPDDHELAIGRQEADPERGGEGWRTYYLYRLADVTGEDLDDASVGFDPQSG
                     SPEVDFTMNTRGASRMGDLTGRSIGKRMAIVLDDRVESAPVIQGQIGARGRITLGAYL
                     DRNQLLQEAKDLVVVLQAGALPAPLRPANEQLIGPTLGQDSVQKGALAALVGILLVLV
                     FMAMYYQVGGLVADAMVLLNLQLLLGTMSGIGATLTLPGVAAIALTVGMAVDANVLIT
                     ERIREELRLGKSPRSAVEQGYARAYSSVFDSQLTTAIAGIVLWQFGTGPIKGFATMLL
                     IGIGTSLFTGTFCSKVLFDWLVRGARVQRLRVG"
     gene            4372..5505
                     /gene="secF"
                     /locus_tag="VFG22_06000"
     CDS             4372..5505
                     /gene="secF"
                     /locus_tag="VFG22_06000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HET6415827.1"
                     /translation="MELIKPGSYFDFMKYRGPVITVLALMATLSLVSLFYPGPNYGID
                     FAGGTEVQLAFRGDTTPNEVRKALENSGYSRPDVISVEGQPNQYIIRVQEVSSLPEEQ
                     VDKLREAVTALLGEDVVREMKVSPGGDKITLRVSGEVHEQQLQEALEAAGARVRSLNL
                     LAGSRDTRYEVHLVGVADELTAQLREKFGDRAPEGALRVEWVGPKAGEQLRDAAIQSL
                     LYAIAFIMLYVAFRFDMRFAPGGVIAMLHDALITLGIYVLLQKEVNLTTVAALLTIMG
                     YSINDTIVVYDRIRENMVRVRDKSLRELINISTSQMLSRTIVTSLTTLLSVSAFFFLG
                     TGVIADIAFALGVGILIGTMSSIFVAAPITEMMDRRFFNRSRS"
     gene            5510..7186
                     /gene="recJ"
                     /locus_tag="VFG22_06005"
     CDS             5510..7186
                     /gene="recJ"
                     /locus_tag="VFG22_06005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HET6415828.1"
                     /translation="MNRYSVRPSDERAAGMLADASGLGLSAAQVLLHRGVRDAEEARP
                     FLEARLRGLSAPGPMADRSLAAERIARAIGAGERIVVFGDYDVDGTTSAVILSEVITA
                     LGGEARTLIANRFEGGYGLSERALDRCLEEGPGLLITCDCGSSDHERIARAISRGVDV
                     IVVDHHLVPEDPLPAFAFLNPHRPDCGFAYKGLCSAGLAFSLGAALRAEMNMELDLRA
                     WLDLVAIGTIADVAPLTGDNRRLVRAGLKSIGSTTSRPALRALRTAAKIPESAELTAR
                     DVAFRFSPRLNAPGRLGVADLTLRFLSAKTPKEAWELLVEVEAQNDERKALADRATQE
                     ARAQVREVYGETPEAGLVVASDTWHRGVVGIVAARLVDTFGVPAVVISFDGEQGHGSV
                     RTTDDFDVYRALSRCASDLSAWGGHRAAAGLSLPRNALDSFRASFLAATEGAGSGALA
                     SREVDVALGGAFRAPSVEDLQSLGPFGEAFPAPTFLVDAHVVESTGVGEGRAHGKLKL
                     RIGQDSVRAFAPGMFSRLEGRDQLRLVGEFQPDHWMGGRSVELLVMDVLD"
     gene            complement(7183..7962)
                     /locus_tag="VFG22_06010"
     CDS             complement(7183..7962)
                     /locus_tag="VFG22_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET6415829.1"
                     /translation="MQDRDYQSLLVEQDGHIVTLTFNNPEKKNPLSQDLVNELLYALD
                     DAEAADEVRVIVLTGAGNAFSAGADLKGMNAERGKLEKKGEFDDLMLRFDTLQKPVVA
                     RIPGYVMGGAMGIVACCHFAIACESAILGTPEIKRGLFPMMIMAVLQRVIHPRDLLGL
                     MLLGEKISARKAKEIGLLSEVVPDDALDARVAELAGQLAKQSPNAMRMGLNAYNEQRR
                     MPLEEALPYLREQLYAVLGSEDAKEGLAAFFQKREPQWTGR"
     gene            8084..8692
                     /locus_tag="VFG22_06015"
     CDS             8084..8692
                     /locus_tag="VFG22_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415830.1"
                     /translation="MSRERIGYAFALLTVISLSASPCFVHAQSDPAQFTSVDATPKGT
                     IGLGLVGAELGLIIPSLSGLNEPWSLAVFPVVGAAGGALAGYFALDKPNREKGSVAAL
                     VVGLAGVLPAILVTVKGVRAERQSRWEPQPQIELRSEKEQRAFETAEAGPGLLRRSRS
                     GLRVAMPSVSVSRRAPQGRETASMGGLRDRTEMRLSLASGVF"
     gene            8702..9664
                     /gene="tilS"
                     /locus_tag="VFG22_06020"
     CDS             8702..9664
                     /gene="tilS"
                     /locus_tag="VFG22_06020"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HET6415831.1"
                     /translation="MISSKVRRTVRERELLKAGDHVLVACSGGPDSTVLLHVLHQLRR
                     ELGVTLSAASVDHALRPESASEVERVGAFAKELGIPFFPSRVEVTRQGASIQAQARQL
                     RYQALREIAASRGASIIAVGHTQDDQAETVLARMLRGAGLRGLGGIEPRRADGVVRPL
                     LDCGRSEVRAYALERALPFVDDPSNHQRAFERVRIRHEVLPGLLREDPRIVEHLSALS
                     DEVAEVNAYLDEQLPELPMAGERFIPAEALAELAPPLRIRWLRAWISRETGLSSARSH
                     LTELGRLLTGGGEVLLGSGWSVCRRSGGLSLEYREHRRTRSNRS"
     gene            9775..11721
                     /gene="ftsH"
                     /locus_tag="VFG22_06025"
     CDS             9775..11721
                     /gene="ftsH"
                     /locus_tag="VFG22_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET6415832.1"
                     /translation="MKQSQKTLLFWVLVILMCIVIFNFVAREEQPRSVPFSEFITDVS
                     QGKVQKVKIRPQDNSGEYTYWLKSIDESAEPAQRGARNEKVTVGIMGEQINKQLLDNE
                     VSVEYLPEDQNSLWTSILVTWLPMIFLVGILLFFMRQLQASGGKAMSFGKSRARLLNE
                     SQNKVTFKDVAGIDEAKDDCEEIIAFLKDPKKFQRLGGRIPKGVLLMGAPGTGKTLLA
                     RAIAGEAGVPFFSISGSDFVEMFVGVGASRVRDLFEQGKKHAPCIIFIDEIDAVGRHR
                     GSGLGGGHDEREQTLNQLLVEMDGFESSEGVIIIAATNRPDVLDPAILRPGRFDRRII
                     VPRPDLNGRQGILAVHTRRVPLGDDVQLQIIARGTPGFSGADLENLVNEAALLAARQD
                     KELVSMADFEMAKDKVMMGSERRSMVISEHERKTTAYHEAGHTLVAWMLPNHDPIHKV
                     TIIPRGPALGLTMALPEEDRQSYSAEWIRDRIAMALGGRLAEEVVFGQLTTGASDDFK
                     KATQLARSMVTEWGMSTSLGPLSYVEKEETGFLGQSYHKDYSEETAKEIDDEVRRIIH
                     TQYERAKVVIEAHRAELDAIAAALLERETLGREEIEAIMSGNDLPPPVIVSVPTYAEK
                     RRESEEKRKGAIFQPRPREVPSAG"
     gene            11737..12579
                     /gene="folP"
                     /locus_tag="VFG22_06030"
     CDS             11737..12579
                     /gene="folP"
                     /locus_tag="VFG22_06030"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HET6415833.1"
                     /translation="MPELWGVINVTPDSFSDGGRCLDPRDAVSKGRQLLDDGAHVLDV
                     GGESSRPAGHTYGKGFEPVPVSEEIRRVVPVIEVLAEELGARVSVDTVKHEVAVAALA
                     AGAGVVNDVRCAEDPRLAEAAASAGADYVIMHNRGRGELRSPNTDYQDVVEEVLDELL
                     AGAERVERAGVPRGSVWLDPGLGFAKTARQSAILLSSLGRFRKTGYRVLVGPSRKSFI
                     AELAPNSGGELPRADDRLGGTAAAVAICATAGVDAVRVHDVRVMSQLIRFSEALNKLR
                     GGAS"
     gene            12576..13493
                     /gene="cdaA"
                     /locus_tag="VFG22_06035"
     CDS             12576..13493
                     /gene="cdaA"
                     /locus_tag="VFG22_06035"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HET6415834.1"
                     /translation="MIDMLTDLSGGLFGFFRRNPWEVLHDCFDIGIVAVLIYWALVIL
                     RGTRAMQVAIGLCLVFVGYLFARRVGLVTVTTILDSVLAYLAVIVVIIFQSDIRRALA
                     RMGRRPLLRGQRTAKETQTIEEVIKAAGTLAQKRIGALVAFERSAALDEFIERGTEID
                     AAVSKELLHSIFVPSFENPMHDGAVVIREGRVWQAGVFLPMAGTAGGDRSLGARHRAG
                     IGITEETDAVVVVVSEERGAISLCFDGNIVRNLDVASLRDALYGLFYAPKRNRRKSSE
                     RRSTMAPPEPGRAQNQDGVISVKKAEETS"
     gene            13490..14470
                     /locus_tag="VFG22_06040"
     CDS             13490..14470
                     /locus_tag="VFG22_06040"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HET6415835.1"
                     /translation="MSGLVDAFNAIRLLFTENLGLKASALGIAILMFSLVHGAEDMER
                     NVYVDVVVQPPPDAKDMILVTEVPDRVRVRLKGSRARLNAIRQENLPPVDVELKTREE
                     PQYYFEKEVFDLPTGVTVMGVVPPSVVFKWVPRGVRDLPVQLFFDGELRAGLEWATDP
                     EVFPQTIMVEGPRDVVNSMRTVRSMDLDVEALEEGVVHREIPLVGAPAYTRFEPQTVL
                     VTLRVQQKMKERVLSPLRVDVEGVVPRSLRPQAVTIRVRGPELLIGELDPASVLAIAN
                     VVAAPDDKGTRSVPVEIRGLPDGVELLEAVPANVTVLLGHPDPRRSSERP"
     gene            complement(14454..15821)
                     /gene="glmM"
                     /locus_tag="VFG22_06045"
     CDS             complement(14454..15821)
                     /gene="glmM"
                     /locus_tag="VFG22_06045"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF008139.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HET6415836.1"
                     /translation="MSRNLFGTDGIRGLANRGDMTPEVAFRIGAAIAYQAGKRVEHVP
                     RIVVGKDTRLSGYLFETAVASGICALGGEVLLSGPLPTPAIAHLTTSMRADAGVVISA
                     SHNPYQDNGIKIFGPDGFKLPDEVEKEIEALIFGSELDEKRPTGTRVGRAERLDDAPG
                     RYVAFVKASFPNELTLEGVRIVVDAAHGAAYRTAPLVFSELGATTHTIGVRPNGKNIN
                     HKVGALHPDACAREVRKRGADIGIALDGDADRIIMIDEKGNRIDGDAIMALCATRMLR
                     AGRLRRRTLAATVMSNLGLERAIEADKGKLLRCDVGDRYVVEAMRKRGLNFGGEQSGH
                     LIFLDHATTGDGLVAAMQVLAIVLREGRPLSELASEAMSRVPQVLVNVTLPDRRPLAE
                     MPKTSRAIARVEKSLGKSGRVLVRWSGTEPKLRVMIEGPKQDRITTMANSIAEEATKE
                     FKVAR"
     gene            complement(15829..>17811)
                     /locus_tag="VFG22_06050"
     CDS             complement(15829..>17811)
                     /locus_tag="VFG22_06050"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HET6415837.1"
                     /translation="GGAFKTRGDFHSSLDWMSDSAEGLCGGDAYPRATLRAQLEEGVT
                     RVSMEGVVGEEKVLDLQASIETPLEDWLSSAEIPSWPVTQISADFYEAPTENLPYLCR
                     FAAGVLSAQLKAKDLFGEDPKLAFSLKSDDLRARRLEPARRTGMVNTIVETPPSITRI
                     SGGYENGLGEIDAEMQWWNGGSTTILASVPLRWDKQAPIPTLVERGEIDATAEFDRMP
                     LQAVLAWMAGVVNVEGILQGSVTAQGRGEDPRFVGSLDLSDGRVNLRSVGQTLEDVTG
                     RVIFDEDGVMISDLTAADTGGTAKVDGRVGFKRFKLNKVDFDVRTTNFPLRQEGSILA
                     RVDGDARLRAQFKDEGLIGEVRLRKLEVDIPENTATPLDLERHPDVFFVGETEEPVRR
                     ADAYRVELSIISEDKLIIRSRDQGFFVEASANLKTTLTDEVRVAGAVNLRRGNFQVFG
                     KRFEIRSGSMIFDGDSEMDAKVDLVARHTLRGSNDNVTVVVSGRLSAPTIEFKSSIPT
                     TSEAQVIALLVTGTTRQQRGINTSTAQASQETTNFLTGVAAGLFSASLQSQFGGLAPT
                     FGITQGEGVADDVAGDTAVQVGFNVNSVLPDDIPIRGLYVEGQFVARRNEGGPNTTAQ
                     AQRPGFLIEALWPLNFVTTGTFAPPSNWSIDVTWEP"
BASE COUNT         3160 a         5629 c         5632 g         3390 t
ORIGIN      
        1 ccaaggtacg cgccgacaaa ggcgagctca gctacggcga aaagaagctc atggagcaag
       61 cccgaaagct cttgatcacg gagctcgcgc tggcccgtcg ggtccgcgag gaaacgatcg
      121 agcgcgatat cgacgagatc ctgcactgag ggcggcgaga gcgcgccttt ggcgacagtc
      181 gattcgggcg ggggttagcg cttggctttc tcctcgtgtc cggactggcc aaaccgtttg
      241 gagagctcgc tgaagagcgc gcggagagag accccacgtg agaccaggcc gaccaagagg
      301 tggtagatca catcgcccgc ttcggaggct acccgctcat cgctttcgcc gatgagagcc
      361 tgacccagct ccgcggcctc ctcccgaagc tttgcgtcga tcttcgtggg tccggcgtcg
      421 agcagcgcct tggtgtagct cttgccggcg tccgacgctt tgcgggcctc cagtgtgcgc
      481 tcgagacgca acaaggtcgg cgcagcactc tgttcgccgg cttcgatgag ctcgccctcg
      541 gcgttcagcc gacggaagaa acaggtctcc gcgccggtgt ggcaagaggg gccggccgga
      601 tcgaccatgt aaacgagggt gtcaccgtcg cagtcgaccc atacgctacg taccccgagg
      661 gtgttgccgc tgctctcgcc cttcttccag ggttgctgac gcgagcggct gaagaagtgg
      721 gccgcccggg tcgcgagtgt tcgctcgatg gccgctctgt cagcgtacgc catcatgcgc
      781 acctcgccgg tctccgcgtc ctgcgcaatg accgcaacga ggccctgatc gtcgtacttg
      841 agcgcgtcga gcgacatgag cgggaggtac gccaggtcgc ggacttccgc aagtccggcg
      901 ccagggacag cggcagcaag ccgtgatgcg cggctgctcc accggagggc ggctcgggat
      961 gggcagcgca gcgattgggt cgagcctagc cgtaggtcgt cttgaggagc tgggggcgag
     1021 tgggtcccaa tcgcgagcac gcccgtcgcg agccgccctc cgatcgtccc agcgccgacg
     1081 ctgccccgct aaacgatcga gagccagctc ggctcgggcg tgttcttccc ccgcacagcg
     1141 tcgaaatacg cgctctgtac cttttcggtg atggggccac gggagccctg gccgatgctg
     1201 cgatcgtcga gctcgcgcac gggcgtgacc tctgccgcgg tgccgaccat gaatacctcg
     1261 tcagcgatgt agagctcgtc tcgggagatc aggcgctcgt gaacgatgag gcccaagtct
     1321 cgcatcacgc gcatgaccga gttgcgggta atcccgccga gaatcgatac gcccatcggg
     1381 ggcgtggcga tttctccatc gcgcaccacg aagatgttct cgccggaggc ctccgacacg
     1441 tagccctgtg tgtcgagcat gatcgcctcg tcgtagccga tcgcctggac ttcacgcttg
     1501 gcgagaatcg agttgacgta gtgaccgttg accttgccct tggacatcag cgtgttcacg
     1561 ccggctcgct ggaacgagct caccttcgcg cgaatgcccc gcgtgaggcc ttcctcgccg
     1621 aggtaggcgc cccaacgcca ggcgatgatc gagacacggg tggcgttcgt cttggcgccc
     1681 aggcccattt ctccatcgcc catgaacgcg atgggccgta ggtaacattc gtcgagccga
     1741 ttggcccgaa cggtttcgag gcatgctgcg acgatctccg cctgactgaa gggcatcgga
     1801 agcatgcaga tgtgggcaga atcaaagagg cgtcgaatgt gttcttccaa acgaaagacc
     1861 gcgcttcgcc cgtcttctag cttgtagcag cgaattccct cgaagacgcc cagaccataa
     1921 tggagggtgt gcgtcagtac gtggacgttt gcttgctccc aaggaatgag cttcccgtcg
     1981 agccagatct tgtcgacttt gtcgaccatc gctgctcctt acacccccac caatcggggg
     2041 tcgcgcggtt gtagcataaa tccgcaatta cgctgagttg acctgattgg gggcatggag
     2101 tagaaacgcc ggcgcttcag gagcctggat agcagtgtgc atccgggctt cgcgcgcatt
     2161 gaggttaggg tgctctactc gttatttcac attttgcttc agccggacgt cagcggcggt
     2221 ccggtaacgg cgccaacggg cggaggcggg ccggcgccag gacccatggg ctgtggtggc
     2281 ggcggaaccg agcaaatcgg tttgctcctg ctgatgttcg cggtgctcta cttcctgatg
     2341 atccgacctc agcagaagcg cgccaagcaa cacgaagcga tgatgggggc gctcaaaaaa
     2401 ggagacgtcg ttcgcacgaa cggcggaatc ctcggcgaga tcactgccct cgatgaacga
     2461 gacgtgacga tcgaggtcgc agaccgcacg cgaatcaagg tgttgcggag ccacattgcg
     2521 gggttggacc aaacccccga acagaaaagc tgaacggaca ggcgaggatc atggatcgag
     2581 gctggtattt tcgtttggca atcgtcgtcg gcacgtccct gctgggtttc cttgccctgt
     2641 ggccgagtgt ggatcgatgg ctgccatgcc cggacatcgt caaagaaacc ttcgagaatc
     2701 agatcaaccc tggtctcgac atcaagggcg gactccggct gatgtatgag gtcgaagtcg
     2761 acgaatacat tcgcgaccgt cgagaccgga tcgccgagca ggtgcagcgt caatgcggcg
     2821 tcttgatagg catcgttccc gaggaagaag tcgacgacat cgacaaggcc accctcacgg
     2881 cgatccagca gcgctgtaag gtcgagcgtg tcgacaagga cggcgacgcg gttcgtgcca
     2941 ttcgcatcac cttctccaac caagaagacg cccagaagct cagcaagacc tgggtaaagg
     3001 actacttccg tgaccttcgc gtcgcgagcg gcgtcggtga cctcgtcgtc gaccttcaca
     3061 tgcgcgagga gcagcttgcg aacatgcgcg aaacggccgt caggcagtca gagcgcacca
     3121 tcaccaaccg aatcgacaat ctcggcgtct tggagccgac cgtgatcgcc cgaccgaatg
     3181 acggcgacat catcatcgag attccgggtg cgcaagagag ctccttcgag cgcatcaagt
     3241 cgatcatcag ccgcaccgca caacttcagt tccagatcgt ggacgacgag tccgattcgc
     3301 gctcggtctt caccgcgcca cccgaaggca tatacctcga cgccgactcc caaacctttg
     3361 cggaggcctt tggcgaggac gctcgggacc gactgcgcag ctatatcgcc acacttccgg
     3421 tgcccgatga ccacgagctc gccatcggcc gtcaggaagc ggaccccgaa aggggaggcg
     3481 aaggctggcg tacctattac ctgtaccgcc tagccgacgt gaccggtgag gatctcgacg
     3541 acgcctcggt cggcttcgac cctcagagcg gcagcccgga ggtcgacttc acgatgaaca
     3601 cgcgcggcgc ctcgcggatg ggcgacctca cgggccggag catcggcaag cgcatggcca
     3661 tcgttctcga cgaccgggtt gaatcggcgc cggtcatcca gggtcagatc ggcgcacgcg
     3721 gccgcattac gctgggcgcc tacctcgatc gaaatcagtt gctccaagag gccaaagatc
     3781 tggtcgtcgt gcttcaggcg ggcgcgcttc ccgccccgct tcgaccggcc aatgagcagc
     3841 tcatcggtcc gacgctcggc caggactcgg tccagaaagg ggcgctcgcg gcgctggtcg
     3901 gcatccttct ggttctcgtc ttcatggcta tgtactacca ggtcggaggc ctcgtcgcgg
     3961 acgcgatggt gctcctcaac ttgcagcttc tgctcggcac catgtccggc atcggtgcga
     4021 cactcacgct gcccggtgtc gccgccatcg cgctcaccgt cggcatggcg gtcgatgcga
     4081 acgtgctcat caccgagcgc attcgcgaag agctgaggct agggaagtcc ccccgctccg
     4141 cggtcgagca ggggtacgcc agggcgtact cctcggtttt cgacagccag ctcactacgg
     4201 cgatcgccgg catagtcctt tggcagttcg gcactggccc gatcaagggc ttcgccacga
     4261 tgctcttgat cggtatcggc acgtcgctgt tcaccggcac gttctgctcg aaggttctct
     4321 ttgattggct ggtgcgcggc gcgcgcgtgc agcgactgcg ggtgggctga catggaactc
     4381 atcaaacccg gtagctattt cgacttcatg aagtaccgcg gaccggtcat cacggtcctc
     4441 gctctcatgg ccacgctgag cctagtttcg ctgttctacc cgggacccaa ctacggcatc
     4501 gattttgccg gcgggaccga ggtccaactc gccttccgcg gcgacacgac tccgaacgag
     4561 gtccgcaagg cgctcgaaaa ctcgggctac tcccgtccgg acgtcatcag cgtcgagggc
     4621 cagcccaacc agtacatcat tcgcgttcaa gaggtgtctt cgctgcccga ggagcaggtc
     4681 gacaagctcc gcgaggcggt gacggcactg ctgggcgagg atgtggttcg cgagatgaag
     4741 gtttcgcccg gcggagacaa gatcacgctt cgcgtctccg gcgaggtcca cgagcagcag
     4801 ctccaagaag cgctcgaggc ggctggagcg cgcgtgcgct cgctcaactt gctcgccggg
     4861 agccgcgaca cccgatacga ggtgcacctc gtcggagtcg cagacgagct cacagcacag
     4921 cttcgagaga agtttggcga ccgggctcca gagggcgcgc tccgagtcga gtgggtcggc
     4981 cccaaggcag gcgagcagct ccgcgacgcg gcgattcaat cgctgctcta cgcgatcgcg
     5041 ttcatcatgc tctacgtggc cttccgcttc gatatgaggt tcgcgcccgg tggcgtcatc
     5101 gcgatgctgc acgacgcgct catcacgctc ggcatctacg tccttctgca aaaggaagtg
     5161 aacctcacga ccgttgccgc gcttctgacg atcatgggct attcgatcaa cgacacgatc
     5221 gtcgtctacg atcgaatccg cgagaacatg gtccgcgttc gggacaagag cctccgtgag
     5281 ctgatcaaca tcagcacctc ccaaatgctt tcgagaacga tcgtcacgtc gctcacgacg
     5341 ctcctgtccg tttccgcgtt cttctttcta ggcaccggtg tcatcgccga catcgccttt
     5401 gcgctcgggg ttggcatttt gatcggcacc atgtcgtcga tcttcgtcgc cgcgcccatc
     5461 accgaaatga tggaccggcg cttcttcaat cgctcacgga gctagtccga tgaaccggta
     5521 cagcgtgcgg ccatccgatg agcgggcggc cggcatgctt gccgacgcct cggggctcgg
     5581 gctcagcgca gcccaggtgc ttctgcaccg gggcgttcgc gacgcggagg aagctcggcc
     5641 gtttctcgag gcgaggctcc gaggtttgag cgctcccgga cccatggccg atcgctcgct
     5701 tgcggccgaa cgcatcgccc gtgcgattgg cgctggggag cgcatcgtcg tgttcggcga
     5761 ctacgacgtc gatggaacca cctcggcggt gattctcagc gaggtcatca ccgccctcgg
     5821 cggagaggcg cgtacgctga tcgccaaccg cttcgagggg ggatacggtt tgagcgagcg
     5881 agccctcgac cgctgtctcg aggaagggcc cggccttctc atcacctgtg actgcggctc
     5941 gagcgaccac gaacgcatcg cccgagcgat ctccagaggc gtcgacgtga tcgtcgtcga
     6001 ccaccatctc gttcccgaag atcctcttcc ggcgttcgcg ttcctcaacc cgcaccggcc
     6061 ggactgtgga tttgcgtaca agggcctttg cagtgccggg ctcgcatttt ccctcggagc
     6121 ggcgctgcgc gcggagatga acatggagct cgacctgcgc gcctggctcg acctggtcgc
     6181 gatcggcacc atcgccgacg tcgcgccctt gacgggtgac aaccgccgct tggttcgggc
     6241 aggcctgaag tcgatcggct cgacgacgag ccgaccggcc ctgcgggcct tgagaacggc
     6301 ggcaaagatt ccggaatccg ccgagctgac ggcacgcgac gtcgcctttc gcttctcgcc
     6361 ccggctcaac gccccgggcc ggctcggggt ggcggacctc acgcttcgct ttctcagcgc
     6421 caagacgccc aaggaggcgt gggagctcct ggtggaggtg gaagcacaaa acgacgagcg
     6481 caaagccctc gccgatcggg ccactcagga agcgcgcgcg caagtgcgag aggtctacgg
     6541 tgagacgccc gaggccggtc tggtggtcgc gagcgatacc tggcatcgag gcgtcgtcgg
     6601 catcgtcgct gcccggctcg tggacacgtt cggcgttccg gctgtagtca tctcgttcga
     6661 cggagagcag gggcacggaa gcgtccgcac caccgacgac ttcgacgtct atcgagcgct
     6721 ctcgcggtgc gcatcggatc tgagcgcatg gggaggccac cgggcggcgg caggtctttc
     6781 tctgccgcga aacgccctcg attcctttcg cgcctcgttc cttgcagcga ctgagggtgc
     6841 gggttctggc gcattggcgt cgcgcgaggt cgacgtagcc ctcggagggg cattccgcgc
     6901 tccctcagtc gaggacctcc agagcctcgg gccgttcggc gaagccttcc cagcgccgac
     6961 gtttctcgtc gatgcccatg tcgtcgagtc aacgggcgtg ggggaagggc gcgcgcacgg
     7021 aaagctcaag ctgcgcatcg ggcaagattc agtacgtgcg ttcgcgcccg ggatgttctc
     7081 tcgactcgaa ggccgcgatc agctccgatt ggttggcgaa tttcaacccg atcactggat
     7141 gggcggtcgc tcggtcgagc tgctcgtgat ggacgtcctc gactagcgtc cggtccactg
     7201 aggctcgcgt ttctgaaaga aagcagccag tccttctttg gcgtcctcgc ttccgagcac
     7261 cgcgtagagc tgttcccgga ggtagggcag tgcctcttcc aacggcatcc tgcgctgctc
     7321 gttgtaggcg ttgagcccca tgcgcatcgc gttgggtgac tgcttggcaa gctggccggc
     7381 gagctccgcc acgcgagcgt cgagggcgtc gtccgggacg acttcgctga gcaggccgat
     7441 ctccttggcc ttgcgggcgc tgatcttctc acccaagagc atgagaccga gtaggtcgcg
     7501 cgggtgaatc acgcgctgca agacggccat gatcatcatc ggaaacaagc cccgcttgat
     7561 ttccggcgtt cccaggatcg cgctttcgca agcgatggca aagtgacagc aggcgacgat
     7621 gcccatggcg ccgcccatca catatccggg gatcctggcc acgacgggct tctggagcgt
     7681 gtcgaagcga agcattaggt cgtcgaactc acccttcttt tcgagttttc cgcgctcggc
     7741 gttcattccc ttgaggtccg ccccggcgct gaatgcgttt cctgctcccg tgaggacgat
     7801 cacccgcacc tcgtctgcgg cctccgcatc gtcgagcgca taaagcagct cgttgaccaa
     7861 gtcctggctg agagggttct tcttctccgg attgttgaac gtcagggtga cgatgtggcc
     7921 gtcctgctcc acgagcagcg attgatagtc tcggtcctgc atgcgcggag ttttgggcgg
     7981 cggttcctcg aattcaaggc aaatgattcc ccgaataaca tcgcatggtt gcggcggttt
     8041 cgagctgcgt gtaatgtgag ccgaggcaaa gccggagggg gatttgagcc gggaacggat
     8101 cgggtatgcg tttgcgctac tgacggtgat ttcactgtcg gcatcgcctt gcttcgtcca
     8161 cgcgcagtcc gatccagctc agtttacgtc cgtcgacgcg acacccaagg gcacgatcgg
     8221 cttgggcttg gttggcgccg agctcggtct gatcattccc tcgctctctg gcctcaacga
     8281 gccctggtcg cttgcggtgt tcccggtcgt gggcgctgcg ggcggcgcgc ttgctggcta
     8341 cttcgccctc gacaaaccga atcgcgaaaa gggctccgtg gctgcgctcg tcgtcggttt
     8401 ggcaggtgtg cttcctgcca tcctcgtcac ggtgaagggc gtacgcgcgg agcggcaaag
     8461 ccgctgggag ccccagccac agatcgagct gcgaagcgaa aaggagcagc gcgctttcga
     8521 gaccgccgaa gcagggcccg ggctccttcg tcgctcccgc tcgggcctcc gcgtcgccat
     8581 gccctccgtt tccgtttctc ggcgcgctcc gcagggcagg gaaacagcct cgatgggggg
     8641 cctccgggat cgcaccgaga tgaggctttc cctggcctcg ggcgtgttct agcgtctccg
     8701 catgatctct tcgaaggtgc gacggaccgt gcgggagcgc gagctgctga aggccggcga
     8761 ccacgttctg gtcgcatgct ccggcggccc cgattcgacc gtgctcttgc acgtcttgca
     8821 ccagctccgg cgcgagcttg gcgtcacgct ctccgcggct tcggtcgatc atgcccttcg
     8881 tcctgaatcg gcctcggagg tggaacgagt cggagccttc gcgaaggagc tgggaattcc
     8941 cttcttcccg tcgcgcgtcg aggtgacccg gcaaggcgct tcgattcaag cgcaggcgcg
     9001 acagctcaga taccaggcgc tccgagagat cgcggcctca cggggtgcat cgatcatcgc
     9061 cgtgggccac acccaggatg accaggcgga aaccgtcttg gcgcgtatgc tcaggggtgc
     9121 agggcttcgc gggctcggcg ggatcgagcc tcggagagcc gatggagtcg tccggccgct
     9181 cttggattgc gggcgttccg aggtccgtgc ctacgcgctc gaacgtgcgc ttccattcgt
     9241 cgacgatccg tccaatcacc agcgcgcctt cgagcgcgtt cgaattcgtc acgaggtgct
     9301 ccccgggctt ctccgcgagg atcctcgaat tgtcgagcat ctgagcgcgc tgagtgatga
     9361 ggttgcagag gtgaatgcct acctggacga gcagctcccc gagctgccca tggccggtga
     9421 gcgcttcatc cctgccgaag cgctcgccga gctggcacct ccgctccgaa ttcgctggct
     9481 tcgcgcctgg atctcgaggg aaaccggcct cagcagcgcc cgcagccatc taaccgagct
     9541 cggccgactt cttacgggcg gaggcgaggt cctgctagga tcaggatggt cggtctgtcg
     9601 ccggagcggc gggctttcgt tggaatatcg tgagcatcgt agaacacgta gcaatcgttc
     9661 ctgagccttc ggagaggcga tggttgccaa tcggcctccg cggcctcaca aagatggaag
     9721 gggccgtggc tccacgctag tccgaaacct cggccccgcg tcccgcctta tcatatgaag
     9781 caaagccaaa aaacactgct cttctgggtc ctcgtgatcc tgatgtgcat cgtgatcttc
     9841 aatttcgtcg cacgtgagga gcagccgcgg agcgttccgt tcagcgagtt catcaccgac
     9901 gtttcgcaag gcaaggtcca gaaggtcaag atccgcccgc aggacaacag tggcgagtat
     9961 acctactggc tcaagtcgat cgatgaaagc gcggagccag cccagcgcgg tgcacgcaac
    10021 gagaaggtca ccgtcggcat catgggcgag cagatcaaca agcagttgct cgataacgag
    10081 gtgagcgtcg agtacctgcc cgaagatcag aacagcctat ggacgagcat tctcgtgact
    10141 tggcttccga tgatcttcct cgtgggcatc cttctatttt tcatgcgtca acttcaggcc
    10201 tcgggcggca aagcgatgag cttcggcaaa tcgcgcgccc gcctgctcaa cgagtcccag
    10261 aacaaagtca ccttcaaaga cgttgctggc atcgacgagg ccaaggacga ctgcgaagag
    10321 atcattgcct tcctgaaaga cccgaagaag tttcagcgcc tcggcggccg catcccaaag
    10381 ggcgtcttgc tcatgggggc cccgggaacc ggcaagacgc tcctggcccg cgcgatcgca
    10441 ggcgaggcgg gcgtgccgtt cttcagcatc tcaggctcgg atttcgtcga gatgttcgtg
    10501 ggtgttggcg cgtcccgcgt tcgtgacctc ttcgagcagg gcaagaagca tgccccttgc
    10561 atcatcttca tcgacgagat cgacgccgtc gggcgccatc gtgggtcagg cctcggcggc
    10621 ggccacgacg agcgtgaaca aacgctgaac cagctcttgg tcgagatgga cgggttcgag
    10681 tcgagcgaag gcgtcatcat catcgcagcg accaaccgcc ccgacgttct cgacccggcg
    10741 attcttcgac ccggtcgctt cgatcgccga atcatcgtgc cgcgtcccga cttgaacgga
    10801 aggcagggaa tcctcgcggt gcacacccgg cgcgttcctc tgggggacga cgtgcagctc
    10861 cagatcatcg cccgaggcac acccggcttt tccggtgccg acctcgagaa cctggtcaac
    10921 gaggccgcgc tcctggctgc gcgccaggac aaagagctgg tttccatggc tgatttcgaa
    10981 atggccaagg acaaggtcat gatgggctcc gagcgccgct cgatggtcat cagtgagcac
    11041 gagcgaaaga ccacggcgta ccacgaagcg ggccacactc tggtcgcgtg gatgcttccg
    11101 aaccatgacc cgattcacaa ggtcacgatc attccgcgcg gtcccgcgct cggcctcacg
    11161 atggccttgc ccgaggagga ccgccagagc tacagcgccg agtggatacg cgatcgcatc
    11221 gccatggcgc tcggtgggcg tctcgcggaa gaggtcgtgt tcggtcaact caccaccggt
    11281 gcttcggacg acttcaagaa ggcgacccag ctcgcgcgct ccatggtgac cgagtggggt
    11341 atgagcacga gtctcggtcc tctctcgtac gtcgagaaag aggagacagg ctttctcgga
    11401 cagtcctatc acaaggacta ttccgaggag acggccaagg agatcgacga cgaggttcgc
    11461 cggatcatcc acacccagta cgagcgcgcc aaagtggtga tcgaggcgca tcgagcggag
    11521 ctcgacgcaa tcgcggcggc cctgctggag cgtgagacgc tcgggcgtga ggagatcgaa
    11581 gcgatcatgt ccggcaacga tttgccgccg ccggtcatcg tgagcgtacc cacgtacgca
    11641 gagaagcgtc gcgagagcga agagaagcga aagggggcga tctttcagcc ccgcccgcga
    11701 gaagtcccta gcgcagggta ggtgacggag gtcccgatgc ccgagctgtg gggcgtcatc
    11761 aatgtgacgc cagattcgtt cagcgacggt gggaggtgct tggacccccg agacgcggtc
    11821 tccaaggggc gccagctgct cgatgacggt gcccacgtcc tggacgtcgg cggcgagtcc
    11881 tcacgaccag ccgggcatac gtacggcaag ggtttcgagc cggttcccgt gagcgaagag
    11941 atccgtcggg tagtgcccgt gatcgaggtg ctggctgagg agctgggagc gcgcgtttcg
    12001 gtggacacgg tgaagcacga ggttgcagtc gcagccttgg cggcgggtgc aggggtcgtc
    12061 aatgacgttc gctgcgcgga agatcccagg cttgcggaag cggcggcttc ggcaggcgcc
    12121 gactacgtga tcatgcacaa ccgaggccgg ggcgagctcc gctcacccaa caccgactac
    12181 caggacgtcg tcgaggaggt gctcgacgag ctcttggccg gggctgaacg cgtggagcgt
    12241 gccggcgtcc cgagggggtc cgtctggctc gatcctgggc tggggtttgc caagacggcc
    12301 cgccagtccg caatcctcct aagttctctt gggcgtttcc gaaagaccgg ataccgtgta
    12361 cttgtggggc cttcgcggaa atccttcatc gccgagcttg cgccgaattc cggcggcgag
    12421 ctcccgcgcg ccgacgatcg gctgggcggt acagcggccg cggttgcgat ctgtgcgacc
    12481 gccggcgtcg atgccgtacg cgtccacgac gtgcgcgtga tgagccaact cattcgtttc
    12541 tccgaggcgc taaacaagct tcgaggtggc gcgtcatgat cgacatgctc accgatctca
    12601 gtggcggcct attcggtttc ttccgccgaa acccgtggga agtcctccac gactgcttcg
    12661 acatcgggat cgttgccgtg ctgatctact gggcgctcgt catcttgcgc ggcacccgag
    12721 ccatgcaggt tgccattggc ctttgtctcg tcttcgttgg ctacttattc gcgcgtcgcg
    12781 tcgggctggt gaccgtcacc acgattctcg attcggtgct cgcctatctg gcggtcatcg
    12841 tcgtgatcat cttccaatcg gacattcggc gcgcattggc acgaatgggc cgtagaccgt
    12901 tgctccgcgg acaacgcacc gcgaaggaaa cccaaacaat cgaagaagtg atcaaagccg
    12961 caggcaccct tgcccagaaa cgcatcggtg cgctggttgc attcgagcgc agcgccgcgc
    13021 tggacgagtt catcgaacgc gggaccgaga tcgatgccgc cgtgagcaaa gagctcttgc
    13081 acagtatctt cgttccgagc ttcgaaaacc ccatgcacga cggagccgtc gtcattcggg
    13141 aagggcgcgt ttggcaagcc ggcgtcttct tgccgatggc aggtaccgct ggcggtgacc
    13201 gctccctggg cgcgcgccac cgggcgggca tcgggatcac cgaggagacc gacgcggtcg
    13261 tggtcgtcgt cagtgaagag cgaggcgcga tctcgctctg tttcgacggt aacatcgttc
    13321 gcaacctcga cgtcgcatcg ctacgcgatg cactctacgg cctgttctac gcgcccaagc
    13381 gaaaccgccg taaatcgagc gaacggcgca gcaccatggc acctccggag ccgggccgcg
    13441 cccaaaacca agacggcgtg atcagcgtca agaaggccga ggagacctcg tgagcggcct
    13501 cgtcgatgcg ttcaacgcga ttcggctcct gttcaccgag aatctcgggc tcaaagcgag
    13561 cgcgctaggt atcgccatcc tcatgttctc gctggtgcac ggcgcagaag acatggagcg
    13621 caacgtgtac gtcgacgtcg tggttcaacc gccgcccgat gcgaaggaca tgattctcgt
    13681 gacggaggtt cccgaccgag tgcgggtacg actcaaaggc agccgcgcac ggctcaatgc
    13741 gattaggcaa gagaacctgc cgccggtcga cgtggagctc aagaccaggg aggaacccca
    13801 gtactatttc gagaaagagg tcttcgacct tccgacgggc gtgaccgtga tgggagtggt
    13861 tccgccttcc gtggtgttca aatgggttcc gcgtggcgtt cgtgacctcc cggtgcagct
    13921 gttcttcgat ggagagcttc gcgccgggct cgagtgggcg actgaccccg aggtgtttcc
    13981 acaaaccatc atggtcgaag ggccgcgaga tgtggtcaac tcgatgcgaa cggttcgttc
    14041 gatggatctg gacgtcgagg ccctcgaaga gggtgtcgtc catcgcgaaa ttccgctcgt
    14101 aggggcgccg gcctatacgc gcttcgagcc acagacggtg ctcgtcaccc tgcgggtcca
    14161 gcagaagatg aaggaacgag tgctttcgcc actgcgcgtc gatgtcgagg gtgtcgtgcc
    14221 tcgttccttg cgcccccagg cggtcaccat ccgcgtgcgc ggccccgagt tgctgatcgg
    14281 tgagctcgac ccggcatccg tgctggcaat cgccaacgtc gtcgctgcgc ccgacgacaa
    14341 gggcactcgg tccgtgccgg tggagatccg aggcttgccg gatggcgtcg agctgctcga
    14401 agccgtaccg gcgaacgtca cggttctgct cggccaccca gacccgcgcc gctctagcga
    14461 gcgaccttga actccttcgt ggcttcctca gcgatgctgt ttgccatggt cgtgatgcgg
    14521 tcctgcttgg ggccctcgat catgacccgg agcttcggtt cggtgccgct ccagcgaacg
    14581 agcacgcgtc cgctcttccc aagcgatttc tcgacgcggg cgatggcacg gctcgtcttc
    14641 ggcatctccg cgaggggtct ccggtcgggt agggtgacgt tgaccaacac ctgcggcacg
    14701 cgagacatcg cttcggaggc cagctcggaa agtgggcgtc cctctcgcag cacgatggca
    14761 agcacctgca tggccgccac cagtccatca cccgtcgttg cgtgatccaa gaagatcagg
    14821 tgccctgatt gctcgccgcc gaagttcagg ccgcgtttac gcatcgcttc gactacgtag
    14881 cgatcgccga cgtcgcagcg caagagcttg cctttgtcgg cctcgatggc gcgttccagg
    14941 ccgaggttgc tcatcacggt ggcggctaag gtacgacgac ggaggcgacc cgcgcgaagc
    15001 atacgcgtcg cgcacaatgc catgatcgcg tcgccatcga tccggttccc tttctcgtcg
    15061 atcatgatga tgcggtccgc atctccgtct agggcgatcc cgatgtccgc tcctcgtttc
    15121 cgaacctcgc gtgcgcaggc atccggatgc aacgcaccca ccttgtgatt gatgttcttg
    15181 ccgttcggtc gaacaccaat cgtatgcgtc gtggcgccca actccgaaaa gacgagaggc
    15241 gccgttcggt atgcggcccc atgcgcggca tcgactacga tgcgaactcc ttcgagggtc
    15301 agctcgtttg gaaacgaggc cttcacgaac gcgacgtagc ggcccggcgc gtcgtccaag
    15361 cgctcggcgc gccctacgcg ggtccccgtc gggcgcttct cgtcgagctc ggagccgaag
    15421 atcagggcct cgatctcttt ttcgacctcg tcgggaagct tgaaaccgtc gggcccgaag
    15481 atcttgatgc cgttgtcttg atatggattg tgcgaagcgc tgatcacgac tccggcatcg
    15541 gcgcgcatgc tcgtggtgag gtgcgcgatc gcgggggtgg ggagcggccc cgaaagcaga
    15601 acctctccac cgagggcgca gattcccgag gccacggcgg tttcgaagag gtatcccgag
    15661 aggcgcgtgt cctttcccac cacgatcctc ggcacgtgct cgacgcgttt tccagcttgg
    15721 taagcgatcg cagcgcctat acgaaatgcg acctccggtg tcatgtcgcc gcgattggcc
    15781 aggccgcgaa ttccgtcggt tccaaataga ttccggctca tgatcgattc aaggctccca
    15841 cgtgacgtcg atactccagt tcgatggcgg agcaaaggtc cccgtcgtca cgaagtttag
    15901 tggccaaagc gcttcgatca aaaatccagg gcgctgtgcc tgggccgtgg tgttcggtcc
    15961 gccttcgttg cgtcgcgcga cgaactgtcc ttcgacgtag aggccccgga tgggaatgtc
    16021 gtcgggcagc acgctattca cgttgaatcc aacctggacc gcggtgtcgc ccgctacgtc
    16081 gtccgcaact ccctctccct gggtaattcc aaacgtcggc gcgaggccgc caaactggga
    16141 ttgcaaagaa gccgagaaga gtcccgcagc cacgccggtc aagaagttcg tcgtttcttg
    16201 tgaagcttgc gccgtcgaag tgttgatacc gcgctgttgt cgcgtcgttc cggtgacgag
    16261 aagcgcaatg acctgggctt cgctcgtcgt ggggatgctc gacttgaact cgatggtcgg
    16321 agcgctcagg cgcccggaaa cgaccacggt cacgttgtcg ttgctgcctc tgagagtgtg
    16381 ccgcgcgacc aagtccacct tggcgtccat ttcggagtca ccatcgaaaa tcatgctgcc
    16441 agagcgaatc tcgaagcgct tcccaaagac ctgaaaattg ccgcggcgca gatttacggc
    16501 gcctgcaacc ctgacctcgt cggtcagggt cgttttcagg tttgcgctcg cttcgacgaa
    16561 gaaaccctgg tctctgctgc gaatgatcag tttgtcctcg gagatgatgc tcagctcgac
    16621 tcgataggcg tccgctcgtc gaaccggttc ctcggtttcc ccgacgaaga acacatcggg
    16681 atggcgctcg agatcgagcg gcgtggccgt gttttcgggg atgtcgacct ccaacttacg
    16741 cagccggact tccccgatca gcccctcgtc cttgaactgc gcgcgcaacc gggcatcgcc
    16801 atctacgcgc gctaggattg acccctcctg ccgcaacggg aagttggtcg ttcgaacgtc
    16861 gaagtcgacc ttgttgagct tgaatcgttt gaagccgact ctgccgtcga ccttcgcggt
    16921 gccgcctgta tcggctgccg tgaggtcgct gatcatgact ccgtcctcgt cgaatatcac
    16981 gcggcccgtt acgtcctcga gagtctggcc gacgcttcga aggttgactc tcccgtccga
    17041 gagatcgagg cttcccacga accgcgggtc ttcaccgcga ccctgggccg tcacgcttcc
    17101 ttggagtatc ccttccacat tgaccactcc cgccatccac gcgaggacgg cctgaagcgg
    17161 catccggtcg aactccgccg tggcgtcgat ttcgccccgc tcgaccagag tggggatcgg
    17221 agcttgcttg tcccagcgaa ggggtacgct tgccaagatc gtcgtcgatc caccgttcca
    17281 ccactgcatc tcggcatcga tctcgccgag accgttctcg tacccacccg agatacgagt
    17341 gatcgacgga ggcgtctcta cgatcgtgtt gaccatcccg gttcggcgcg ccggctccaa
    17401 tcgtcgcgca cgcaggtcgt ccgacttcaa agagaaagcc agcttcgggt cctcgccgaa
    17461 caagtccttt gccttgagct gcgctgacaa aacgccggct gcgaagcggc agagatacgg
    17521 caggttctcg gttggagctt cgtagaagtc tgcgctgatc tgggtgaccg gccaggaagg
    17581 gatctcggcg ctgctgagcc aatcctcgag cggcgtctcg atggacgctt gcaagtcgag
    17641 caccttctcc tctcccacca cgccttccat ggaaactctt gtgacgccct cttctaattg
    17701 tgctcgcagc gtagcgcgag ggtaagcatc gccgccgcaa agtccctccg cggagtcgga
    17761 catccaatcc aagctggaat ggaagtcgcc acgggttttg aacgcgcccc c
//
LOCUS       DASRZF010000132         3966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2839230, whole genome shotgun sequence.
ACCESSION   DASRZF010000132 DASRZF010000000
VERSION     DASRZF010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2839230"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2031
                     /locus_tag="VFG22_06055"
     CDS             <1..2031
                     /locus_tag="VFG22_06055"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HET6415838.1"
                     /translation="QIAVSAEEFSNDCAVADIDQDGDLDIVVPDAMLAPDNLFWFENP
                     GPATVLTASSWRRHAIGQTISWCKDLRVVDLDADGRLDVVARPQSRPPQLFFQEESGA
                     WTRVELIGIELGREGMAVGDLDSDGDTDLVIRGAWLQNPGSSLARVPTKWTLHSIGEA
                     PEDFKAFVADVDGDSDEDVLFSSSEDEAPIVWWERTGSDPSGWARHVVAEHASSAHTL
                     WSADLDLDGDLDVLAAELGRSRVTWYENLDGRGDQWRAEVIESRDGAVHNGIVVDLDR
                     DGDLDLFGAGFTGQQTKAAIWWNQLDPKRLRIGPFTAIEVTRRHVRALGSAIVDLNRD
                     GFKDIAAGPYWYRNPGGDMTQPWEQHVFPQTAGGSIDVIASVSVGDEAPSLVAMTPDA
                     RLWWLRPADDGFSITEVGSLPEADHGISTQGVEVAGLLPGDALELVLSNGGDEATGVG
                     VYAFRIVAEGPWPRRRITDRTSDEGIAVGDIDGDGNLDLVGTRGDRGEVEWYANPGGL
                     QSDWSAHPIATLENMSWLDRVGVSDLNCDGRLDVVVTEENGKARGAQTYWLEQPADPR
                     QSNWPAHLVASQGSTNSLDIADFDHDGDMDIVTGEHQGDLKVVVWENTDCGSFEAHLI
                     DSGKESHLGTKAVDLDDDGDIDLVSIAWDEPERIHVWRNDAISPESGISPRP"
     gene            complement(2063..3427)
                     /locus_tag="VFG22_06060"
     CDS             complement(2063..3427)
                     /locus_tag="VFG22_06060"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET6415839.1"
                     /translation="MYEVTELPYDKRPANRDLSHIQGEYGWPFVGKTLDMFKDPQALF
                     AHYYREYGPVSRISITGNKCVLLLHPDHVQRVLMDREQNFSIKMGWESVMADFFQGGL
                     VMRDYDEHRLHRGIMQSSFKPPAMRGYATKIQTIVEKTVERWAAEESILFYKEAKRLL
                     LDIAFEVFCWVDDSENTVPEISRAFTNMMEGALGIVRLDVPGFLYHRGLEGRRYLKRF
                     FMDLVEQKRASDDEDVFAHFCREKTEDGEYYSNEDIADHMVFLMLAAHDTTTGASTMA
                     AYYLANDLPLQDRLASELADWPRELSFDTVFHSTNELICVFYETIRLHPPVPLFLRRT
                     IRECEIGGVHVPADTMICVPGNYIHRLPDWWEEPDSFRPKRFSEEVSEHRKHNFMWVP
                     FGGGAHKCIGMHFARMLFQLTFRELIGKYRIEFVDDDYFPAKLQHFPFTRPVDSLPLR
                     LSAR"
     gene            complement(3435..>3966)
                     /locus_tag="VFG22_06065"
     CDS             complement(3435..>3966)
                     /locus_tag="VFG22_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415840.1"
                     /translation="PSHDTSHDRLSVLRDAHRTLIALADGADSSPGAQTAAARTVNEL
                     ARCFRSGRLPGDQAEWVMVLEAIDHVVLGDPDATETSALVMLVQQGTVTGAFVGESLA
                     YIIPPVGDAKLLTPGGRQAPGIGTGFASPRGFGPVKLDGKLITRRAARFEPDDLPAAI
                     AARSRNGVAKASLQVV"
BASE COUNT          785 a         1156 c         1270 g          755 t
ORIGIN      
        1 cagatcgccg tgtcggcgga agagttctcc aacgactgcg cagttgccga catcgatcaa
       61 gacggcgacc tggacatcgt cgtgcccgac gcgatgctgg cgcccgacaa tctattctgg
      121 ttcgaaaatc ccgggccggc gacggtgctc actgcctcga gctggagacg ccatgcaatt
      181 gggcagacga tctcctggtg taaggacctg cgcgtcgtcg acctggacgc tgatgggcgc
      241 ctcgacgtcg tcgcgcggcc gcagtcgcga ccccctcagc tgttcttcca agaggaaagc
      301 ggcgcatgga cccgagtcga gctcatcgga atcgagttgg gtcgcgaagg gatggcggtc
      361 ggtgacctcg acagcgatgg cgataccgac ctcgtcatca ggggcgcatg gctgcagaac
      421 ccggggtcca gcctggctcg tgtcccaacg aagtggactc ttcattcgat cggcgaagcc
      481 cccgaggact tcaaggcgtt cgtcgcggat gtcgacggag attcagacga agacgtcctg
      541 ttctcctctt ccgaggacga ggccccgatc gtttggtggg aaagaacggg atcggatccg
      601 agcggatggg ctcggcacgt cgtcgctgag catgcgtcgt ccgcacatac tctttggagt
      661 gctgacctgg acctcgatgg agacctagac gtccttgcgg ctgagctcgg gcgttctcga
      721 gtgacttggt acgagaatct cgatggtcgc ggagatcagt ggcgagccga ggtgatcgag
      781 tcacgggacg gagcggttca caacgggatc gtggtcgacc tcgatcgaga cggagacttg
      841 gaccttttcg gagcagggtt caccgggcag cagaccaagg cggcaatttg gtggaaccag
      901 ctcgatccca agcgtctgcg cattgggccc tttaccgcaa tcgaggtgac gagacgccat
      961 gtgcgcgctc tcgggagcgc catcgtcgat ttgaaccgag atggattcaa agacattgcc
     1021 gcgggtccct attggtaccg caatccgggt ggcgacatga cccaaccatg ggagcaacac
     1081 gtatttcctc agactgccgg tggctcaatc gatgtgattg catcggtaag cgtcggagac
     1141 gaagcgccga gcctcgtcgc gatgactccg gatgcaaggc tctggtggtt acggccggcg
     1201 gacgacggct ttagtatcac tgaagttggg agtcttcccg aagcggacca tggaatcagc
     1261 acccagggtg tcgaggttgc cgggctcctg ccgggcgacg ccctagagct cgtcttgtcc
     1321 aacggcggag atgaagcgac cggggtgggg gtttacgcct ttcgcatcgt cgcggaaggc
     1381 ccatggccta gaagacggat caccgatcgc acatccgacg agggtatcgc cgtgggtgac
     1441 atcgacgggg atggaaatct ggatctggtc ggaacgcgag gagatcgtgg ggaagtcgag
     1501 tggtacgcga acccgggggg tctgcagtca gactggtcag ctcatccaat tgcgacactc
     1561 gagaacatga gctggctcga ccgagtcgga gtgtcggacc tgaactgtga cggccggctc
     1621 gacgtagtgg tgaccgagga aaacggaaag gcgcgcggag cacaaaccta ttggctcgag
     1681 caacccgcgg atccacgcca atcgaattgg cccgcccacc tcgtcgcgag ccaaggctcg
     1741 accaatagcc tggacatcgc ggacttcgat cacgacggcg acatggacat cgtcaccggc
     1801 gagcaccaag gtgacctgaa agtggtcgtg tgggagaaca ccgactgtgg aagcttcgaa
     1861 gcccacctga tcgatagtgg caaggagagt cacctcggca cgaaggcagt cgacttggat
     1921 gacgacgggg acatcgactt ggtcagcatt gcttgggacg agccagagcg gatccatgtg
     1981 tggcgcaacg acgcgatcag tcctgagtcc ggtatctcgc cccgtccatg aaccgtaggc
     2041 ttcaaacagc taggtcagtg ctttaccgtg cgctgagccg caacggaaga ctatccaccg
     2101 gcctcgtaaa aggaaaatgc tgcaacttcg ccgggaagta gtcgtcatcg acgaactcga
     2161 tgcggtactt cccgatcagc tctcgaaagg tcagttggaa gagcatgcga gcgaagtgca
     2221 tgccgatgca cttgtgagcg cccccaccga agggaaccca catgaagttg tgcttgcggt
     2281 gctcgctcac ttcctcggaa aaccgcttcg gtctgaaaga gtcgggttcc tcccaccagt
     2341 ccggcagccg gtggatgtag ttgcccggga cgcagatcat cgtgtcggcc gggacgtgaa
     2401 cgcctccaat ctcacattcg cgaatcgtgc ggcgcaagaa gagtggcacc ggagggtgca
     2461 ggcgaatcgt ttcgtagaag acgcagatga gctcgttcgt cgagtggaac accgtgtcga
     2521 agctgagctc acgcggccaa tcggccagct cggaagccaa tctgtcctga agtggcagat
     2581 cgtttgcgag atagtatgcg gccatggtcg aagcgccggt ggttgtgtcg tgcgctgcga
     2641 gcatcaagaa caccatgtgg tcggcgatgt cctcgttcga atagtactcg ccgtcttccg
     2701 tcttttcgcg gcagaagtgt gcgaacacgt cttcatcgtc ggacgctcgc ttctgctcga
     2761 cgagatccat gaagaagcgc ttgagatagc ggcgtccttc caggcctcgg tgatagagaa
     2821 agcccgggac gtcgagccgg acgattccga gcgcaccttc catcatgttg gtgaacgccc
     2881 ggctgatctc cgggacggtg ttctcggagt cgtccaccca gcagaacacc tcgaacgcga
     2941 tgtcgagcaa gagccgcttt gcttccttgt agaacagaat gctctcctct gcggcccagc
     3001 gctcgaccgt cttctcgacg atggtttgaa tcttcgtcgc atagccgcgc atcgcgggcg
     3061 gcttgaagga gctctgcatg atgccccgat gcaggcggtg ctcgtcgtag tcgcgcatga
     3121 cgagcccacc ctgaaagaag tcggccatga ccgactccca gcccatcttg atcgagaagt
     3181 tttgctcgcg atccatgagc acgcgctgca catggtccgg gtgaagcagg agcacgcact
     3241 tgttcccggt gatcgaaatg cgactgaccg gtccgtactc gcggtagtag tgcgcgaaga
     3301 gcgcctgggg atccttgaac atgtccaagg tcttgccgac gaacggccat ccgtattcgc
     3361 cctggatgtg gctgaggtcg cgattcgccg ggcgtttgtc gtaaggaagc tccgtaactt
     3421 cgtacattca cttactatac gacttgtaaa ctagccttgg caaccccatt ccgactccgg
     3481 gcggcgatgg cggctggcag gtcgtccggc tcgaatcgag cggccctgcg cgtgatgagc
     3541 ttgccatcga gcttcacggg cccgaagcca cgggggctcg cgaaaccggt cccaatcccc
     3601 ggtgcttgcc gtcccccggg ggtgagcagc ttggcgtccc caacgggcgg gatgatgtag
     3661 gcgaggctct ccccgacgaa tgcacccgtg accgtcccct gttgtaccag catcacgagc
     3721 gcgctcgttt cggtggcatc ggggtcgcca agcacgacat ggtcgatcgc ttcaagcacc
     3781 atgacccact cagcctggtc tccgggaagc ctgccgctcc ggaagcacct cgcgagctcg
     3841 ttcacggtgc gtgcggctgc ggtctgcgcg cccggactcg aatctgcgcc gtctgccaag
     3901 gcgatgagag ttcgatgcgc atcgcgcagc accgagagcc ggtcgtggct cgtgtcgtga
     3961 cttgga
//
LOCUS       DASRZF010000133        13868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2496263, whole genome shotgun sequence.
ACCESSION   DASRZF010000133 DASRZF010000000
VERSION     DASRZF010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 13868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13868
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2496263"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VFG22_06070"
     CDS             complement(<1..196)
                     /locus_tag="VFG22_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415841.1"
                     /translation="MASTLKRRRIIVLVHESLVPPEDISDLSPAEIEPFKTEFDVREG
                     LRFLGHEVSMVGLSDELGPLR"
     gene            complement(181..1197)
                     /locus_tag="VFG22_06075"
     CDS             complement(181..1197)
                     /locus_tag="VFG22_06075"
                     /inference="COORDINATES: protein motif:HMM:NF027219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HET6415842.1"
                     /translation="MLIHWENLPTEELLDLRLSELGLEVANTWLEDCVDKLREELDGA
                     GLRRFRLHTWLSDEWCSPDGIPGVGIPFYLAHPRLMRLERQMMFEVEGGTRTECMRLL
                     RHEAGHALQHAYNLQRRKLWQRAFGRASEPYPEFYRPNPSSKGFVEHLDGWYAQAHPV
                     EDFAETFAVWLAPRSNWQEQYAHSAALTKLEYIDDLVAELGSKKPQVTSRARPYSLSR
                     LHYTLRRHYERRQDHYSPGFSDEYDRDLSKIFSNSPRYRNNETAASFLRRNRRQIREQ
                     ISVFTGEYQFTVDQVLRDIIGRCRELRLRLIKGERQTNLDFAIMLTVHTVHLLHRRDT
                     WHPL"
     gene            complement(1262..1921)
                     /locus_tag="VFG22_06080"
     CDS             complement(1262..1921)
                     /locus_tag="VFG22_06080"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6415843.1"
                     /translation="MATVMITGANRGIGLELSRQLAERGDQVISVSRKSSPELAALGV
                     RTFDGVDVTDRQALDSVSAELGDTTLDILINNAGIFTEEPFDALDLDRIRKQFEVNAL
                     GALQVTSAMESHLVKGAKIILITSRMGSIGDNSSGSYYGYRMSKAALNMAGVNLAHDF
                     AKRGISVAILHPGLVATEMTGNYGISTRESAEGLIARIDALQLADSGGFWHANGERLP
                     W"
     gene            complement(1983..2417)
                     /locus_tag="VFG22_06085"
     CDS             complement(1983..2417)
                     /locus_tag="VFG22_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415844.1"
                     /translation="MRVTMNHHATCLGIAAFVAILPGCIFVGDYDDVYEPAPKSITYE
                     VCYSSIDCFAGEYCEELAVPADRYTDYVNAICTVGCFDDLDCPISEFNGLPGACLEHA
                     ILGGPLASRICLERCEFDADCDVPAGFGCEVIAGDRLCVPIY"
     gene            complement(2661..4280)
                     /locus_tag="VFG22_06090"
     CDS             complement(2661..4280)
                     /locus_tag="VFG22_06090"
                     /inference="COORDINATES: protein motif:HMM:NF005511.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET6415845.1"
                     /translation="MSKLTPIVVENADSAQWDDSTDLLVVGLGAAGITTAIEARERGA
                     EVLLLDRFGGGGATSISGGVFYAGGGTHIQAEAGVQDSVDNMYRYLSMEVQDAVSDET
                     LRDFCETSAANLKWLADRGVPFLANLCPVKTSYPTNEYHLYYSGNEPLSPYDEKATPA
                     QRGHRVDGGAFPGANLIEPLRRTAITDGVRIELQARVTRLIVDPAGSVVGAEYRQIRS
                     VYWRAVHRHLHTLETAIAKVAPGIAAWLRKRCDAIEEHHSEPRRVRCKRGVVLCAGGF
                     IFNREMAAEHLPEFMPGLPLGTAGDDGMGIRLGQSVGGKVEHMKRASAWRFLYPPNAF
                     AEGLLINRNGERFANELWYGAKIGEAMVEENHGIATLIIDEELRKRARQQSKPGKLQW
                     FQRAAALMNLYFNCKKAKDLDALAKMLDVPRETLVNTLDEYNAAARGERPDRFLKARE
                     HMHALEQGPYYAIDCSLGSKRHLCAVMTLGGLAIDERTGEVLSENGGPVTGLYAAGRN
                     AAGVCSRQYVSGLSIADCVYSGLRAARHAIP"
     gene            4327..5391
                     /locus_tag="VFG22_06095"
     CDS             4327..5391
                     /locus_tag="VFG22_06095"
                     /inference="COORDINATES: protein motif:HMM:NF013255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET6415846.1"
                     /translation="MELPTHPNQVGPVCLVTGGNGYVGKHLVRRLLELGCEVRALDLA
                     PFVGDARVQSIVGDIRRISDVLPACQGVDTVFHTAAIINTLTLARPSVRREVYGVNVH
                     GTEVVIRACRTAGVKKLIFTSSITVAVDGPIREGTESAPYVGSDGLMDLYSQTKSRAE
                     KLVLEADDPNGLRTAAVRPGGVWGPGEGAMMVRDFLEHLAAGQFKATIGDGSSVTDNV
                     HVDSVVDVHFLVAHKLGEDPNLVGGEAYFVSDDEPINPVAWYRPIVEALGYTWPKVRI
                     PEPVAYAFAYLGELAHYFGGPFPTLTRRGVLSVCRDASFRVDKARAHLGYEPRTSAAE
                     GIPELMQEFRTTHDRMKAAQ"
     gene            5388..6116
                     /locus_tag="VFG22_06100"
     CDS             5388..6116
                     /locus_tag="VFG22_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415847.1"
                     /translation="MIKLIYLLWPREPMEPADRRVILLERCAPLLLQSGARYLQMNID
                     DDLAKVPSPAPTTKLTDPFVAEISIWVEDMAMQSALETVLLDASFHIAGYRVREHIYT
                     DYGGNEHGAARNWPDGSRSPYVISVTPLERPKRIPKDRWMRHWFNRQGPMSEKMQPRS
                     RYVRNVVDEVLTPGATAYEGIVEESWPSKTHMTNPFLFYGARNRFELVKNMAIMMQSV
                     AAFLPIWKITSVTMSEYFVRTPRR"
     gene            6171..6737
                     /locus_tag="VFG22_06105"
     CDS             6171..6737
                     /locus_tag="VFG22_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415848.1"
                     /translation="MHTDRRGFLKITFAGAAALSTMSAGATLSGCTSSAPANGMKLLR
                     PEDLELLRALTPAVMKGKIAPGDSAVIEQTLRSFDTLLVDLSEPVVAGVLQAFDVLNF
                     APTRGLTTGQWSSWTKASLSDAESALARLRDSGIGLLNAIYAAVIRLIASSYYLVPEN
                     AISTGYPGPPAKVAGAAPSIAPSGEVTP"
     gene            6734..8353
                     /locus_tag="VFG22_06110"
     CDS             6734..8353
                     /locus_tag="VFG22_06110"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HET6415849.1"
                     /translation="MRNLSTVTLGSSTDPYNKAEERGWDITDASQLSSALELEADVVI
                     VGTGAGGGTAAEILSKAGLRVVILEAGPLKTSSQFDMQERAAYRDLYQEGTGRGTKDG
                     GMVILQGRSVGGSTTVNWTTSFRTPPETLKFWAEEMGVSGCSVEEMAPYFAHMEERLN
                     IHVWPQPPNANNAMLQRASEKLGYSWDTIHRNVRGCLNLGYCGLGCPVDAKQSMLVTT
                     IPAALDLKAKLIHRARVVRVLREGDVVKGVEAEALTSDVRGTTGQKITVRAVRTVLAG
                     GAINTPAILLRSEVPDPHGRIGKRTFLHPTTLTMGIYDESIGPFYGAPQSIYSDHFQW
                     QSVSTGPVGFKLEVPPLQPAFAAGFYSAAGEELTKSMDQLDHTQCMIALMRDGFHEQS
                     QGGAVEINDKGQPVLDYPINEYLLEGVRRAWNVMLEMQFATGAKSARPAHKQATHYRS
                     WNEAKAAVAELEFAAHKVTIGSAHVMGGCPMGDDPERSVTDAAGKFHQLENLYIMDGS
                     LFPTSIGANPQLSIYGLVYKLSSNLAAQFRA"
     gene            complement(8375..9415)
                     /locus_tag="VFG22_06115"
     CDS             complement(8375..9415)
                     /locus_tag="VFG22_06115"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6415850.1"
                     /translation="MSDAEHRQIESNGIRLHAVTQGEGPAILFCHGFPGLWYSWRHQL
                     PVVADAGYRAIALDMRGYGRSDRPAHASEYGNQTIVADLTGVLDALGEDQAVFVGHDF
                     GAQAAWAAALHAPERVRGLVSIAVPYGVGFARRRPKDSKDPTRPSGTRTRGHKPSDTY
                     AEIAKDHFLHLHYFQERGPAEAELGANPRLFLKRIYWALSARGSLLDFKKFPSEGTGY
                     LNVLAEPGEPLPWPWLSEEDLDYLVAEYMRTGPETAFIGGLNSYRAMDVNWAQDPDYG
                     TSPIKQPALFVCGERDPVLQIITPKTLETMPNRVPDLRGVQLIPHAGHFAQQEQPEAV
                     NQSLLRFLATLD"
     gene            complement(9435..10436)
                     /gene="dctP"
                     /locus_tag="VFG22_06120"
     CDS             complement(9435..10436)
                     /gene="dctP"
                     /locus_tag="VFG22_06120"
                     /inference="COORDINATES: protein motif:HMM:NF037995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HET6415851.1"
                     /translation="MNRRLLPILLLVAAALALPGTNTPTKAEAAYTLRIASLAPAGSS
                     WMKILNAWNTTLQEKTDGKVKMRFYPGGSQGDERDFVRKMRVGQLDGGVVTMTGMSML
                     VPAMNVLTLPGFLDTYEELDRVRDKMATEFEGMFSKEGMRLVGWGDAGKTRLFSVQPI
                     KRPSDIKSMRPWVWKDDPIFVEFYQVIGASAVRLGVPEVYPALQTRMVDVISSSALTA
                     VALQWYTRVKYMTAHNSAIIAGGTVMRQDKYDELPPELQEAFNSTAARAHELLNKTIR
                     KDDEKAYQIVLKKGIQAVEAGDAKPEWDAADKKVRDNLTGRMFSKSLVAAVAEAAKP"
     gene            10664..11008
                     /locus_tag="VFG22_06125"
     CDS             10664..11008
                     /locus_tag="VFG22_06125"
                     /inference="COORDINATES: protein motif:HMM:NF014687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HET6415852.1"
                     /translation="MVGGLVENRESTARDHLANERTFLAWVRTALGLVGLGVLLERLV
                     ADGGAIAVAAGIGFIGFGGLSLIYAVSRYIRVAKNLERGMFPVAVRGPLVIAFGALIV
                     TAGAMLYVLLLR"
     gene            complement(11050..11580)
                     /locus_tag="VFG22_06130"
     CDS             complement(11050..11580)
                     /locus_tag="VFG22_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415853.1"
                     /translation="MMNARPIVLLAWVTICTASLLGCSKSQKAPLPESSLPAATLTNI
                     NDVLSAYEVVRGALAKDQSDVRTEALELASAARSGMESAPEGLRLPLEELSGASQRLA
                     ELDGTDLRRARSAFGEVSRELIALLTAEPGLQEGLWVYDCPMAEGYSLWVQADEKVSN
                     PYMGSEMLQCGKVAEF"
     gene            complement(11577..>13868)
                     /locus_tag="VFG22_06135"
     CDS             complement(11577..>13868)
                     /locus_tag="VFG22_06135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HET6415854.1"
                     /translation="IVVMRYGENALSVIDGIQQKLDELGASLPDGVELVITYDRSELI
                     KRAIGTLRNTLIEEMVVVSVIIFLFLLHARSALVPIITLPLAVMLSFIPMFYQGLTAN
                     IMSLGGIAVAIGAMVDASIIVIENIHKKLENWDDGGQQGPRSEVIVRAMQEVGPSLFF
                     SLLVITVSFLPVFTLESAEGRLFKPLAFTKTYSMGFAAILSVTLIPALAVMLIREKIR
                     HEEDNPINRWLISAYAPVVRFVVAHARGVIAFAVVAMAVTIPAFLALQSEFMPPLNEG
                     TILYMPTAPPGMSVTEAANVLQAMDRELKAFPEVVSVFGKAGRAETATDPAPIGMFET
                     TIVLKPRSEWRPGLTWDDLIKEMDQTLRYPGMPNIFWMPVQTRTEMLATGIRSPLGIQ
                     VFGNDLETIERTAVQIEAAVSKIPGTRSAFAERSVGGFFIDFKVDRDKAARYGLRVAD
                     INEVVMTAIGGMKVSETVEGRERYPVTVRYAREFRDNPSLLSNILISTKTGAQVPISQ
                     VATLDFVTGPPMVRSEDGKLVGFVFVDTDRPIAEYVRDAKRAVSEQVELPAGVWLGWA
                     GQFKYLERAKEKLKLVVPVTLLLVLLLLYWNTKSWTETWIVMLAVPFSLIGAVWLLFL
                     LDYNMSVAVWVGMIALAGLDAETGVIMLLYLTLAHRKWSEEGKLRSPDDLTEAIVDGA
                     ARRIRPKLMTVMTTTIALLPILWSTGTGADVMKRIAAPMVGGLLTSFFLELTVYPAIF
                     SIWKRGSLSTLQLDDVAENGGIR"
BASE COUNT         2583 a         4244 c         4289 g         2752 t
ORIGIN      
        1 tcctcagcgg cccaagctcg tcgctgagtc ccaccatgga gacctcgtgt cccagaaagc
       61 gaagcccttc tcgcacgtcg aattcggtct tgaaaggctc gatctccgcg ggcgagaggt
      121 cgctgatgtc ttcaggaggc accaggctct catggacgag aacgatgatt cgccgtcgct
      181 ttagagtgga tgccatgtgt cccgccggtg cagaaggtgc actgtgtgca ccgtcaacat
      241 gatggcaaag tcgaggttcg tctgacgttc ccccttgatg agccgcagcc gaagctcgcg
      301 acaccgtccg atgatatcac gaagcacttg atccacggtg aactgatact caccggtgaa
      361 aaccgagatt tgctcccgaa tttgtcgtcg attgcgccgc agaaacgagg ccgccgtctc
      421 gttgtttcga taccggggcg agttgctgaa aatcttgctg aggtcacggt cgtactcatc
      481 gctgaaaccc gggctgtagt gatcctgacg gcgctcgtag tgccgccgca gcgtgtagtg
      541 cagccgagac aatgaatatg ggcgagcccg agaagtcacc tggggcttct ttgaaccgag
      601 ctccgcaacc aggtcgtcga tgtactccag cttcgtgagc gctgccgaat gcgcatactg
      661 ttcttgccag ttggagcggg gggcgagcca aacggcgaag gtttcggcaa aatcctctac
      721 gggatgggcc tgcgcgtacc atccatccaa gtgctcgacg aatcccttgc tcgacggatt
      781 tgggcgatag aactcggggt agggctcgga cgcccggccg aacgcgcgct gccagagctt
      841 tcgccgttgg aggttgtagg catgttgcaa cgcatgacct gcttcgtgcc ggagcagacg
      901 catgcattcg gtgcgcgttc caccctcgac ctcgaacatc atctgccgct cgaggcgcat
      961 caatctcggg tgtgccagat agaacggtat gccaactccg gggatgccgt cgggagaaca
     1021 ccactcgtcg ctcaaccagg tgtgaaggcg gaagcgccga agccctgcgc cgtcgagctc
     1081 ttcccgaagc ttgtcgacgc aatcctccag ccaggtgttc gctacttcga gacccagctc
     1141 ggaaagccga aggtcgagaa gctcttcggt ggggagattt tcccagtgga tcagcaagat
     1201 tcgggcagct tgacggatct gagtttgtgc gacaacaggc ggcaacgcgc tggctacgcg
     1261 gttaccaggg cagcctttcg ccattggcgt gccagaagcc gccagagtca gcgagctgca
     1321 gcgcgtcgat gcgcgcgatc agtccctcgg ccgattcgcg ggtagaaatc ccgtagtttc
     1381 cggtcatctc agtcgcgacg agacctgggt ggagaattgc aaccgagatc ccacgcttcg
     1441 cgaaatcgtg agcgaggttg actcccgcca tgttgagagc ggccttggac atgcgatatc
     1501 cgtaatagct ccccgagctg ttgtctccga tcgagcccat ccggctggta atgaggatga
     1561 tcttggcgcc tttgacgagg tgactctcca tggcggaagt gacttgcagc gcgccaagcg
     1621 cgttgacctc gaattgcttg cggatccgat cgaggtcgag cgcgtcgaaa ggctcctcgg
     1681 tgaagatgcc cgcgttgttg atcaagatgt cgagcgtggt gtcgccgagc tctgctgaaa
     1741 ccgagtccaa ggcttgccgg tcggtgacgt cgacaccatc gaacgtgcgc acaccgagcg
     1801 ccgcgagctc cgggctcgac ttgcgactga ccgagatgac ctgatcgccc cgttcggcca
     1861 gttgccggct gagctcgagg ccgatgcccc ggtttgcgcc tgtgatcatt actgttgcca
     1921 tgaagcccgc ctttgttccg aggctaacat agcgccctgc tcgattcgtc gatgggttcg
     1981 cgtcagtaaa tggggacgca gaggcgatct cctgcgatca cctcacagcc aaaacccgcg
     2041 ggcacatcgc agtccgcatc gaattcacag cgctcgaggc agatgcgcga cgccagggga
     2101 ccacccagga tagcgtgctc gaggcaggcc ccgggtaagc cattgaactc ggagatcggg
     2161 caatcgaggt cgtcgaagca accgacagta cagatggcgt tgacgtagtc cgtgtagcga
     2221 tcggcgggca ctgcgagctc ttcgcagtac tcgccggcaa agcagtcgat gctcgagtag
     2281 cagacttcgt aggtgatcga cttcggagcg ggctcgtaca cgtcgtcgta gtcgcccacg
     2341 aagatgcatc cggggagtat cgcaacgaag gcggcgattc ctaggcatgt cgcgtggtga
     2401 ttcatggtga ctcgcatggc gtatttcctc tcttaggaga tagacgagcg agccgggcgc
     2461 cgattcaacg ccccctggcc accgagaaac ccagctgatt tcggcccggg gctcgcaagc
     2521 ttcgagtccg actctggcat ccctgtcata gcccgcgcgg gcctcgtatc ttgcgtcttg
     2581 cagcgagcgc agcactcgag gtcagcggct gctgaatcga gagggacccg gtacgaagag
     2641 gctgtcgcct cttcacgggg ttacgggatc gcgtgcctgg cggcgcgcaa gcccgaatac
     2701 acacagtcgg ctatcgagag accgctgacg tactggcgtg agcagacacc ggcggcgttt
     2761 cgccccgcgg cgtagaggcc cgtgactggg cctccatttt cactcagcac ctctccggtt
     2821 ctctcgtcga tcgccaggcc gccgagggtc atgaccgcgc agaggtgccg tttcgagcca
     2881 agcgagcagt cgatcgcata gtagggcccc tgctccagtg catgcatatg ctcgcgggct
     2941 ttgagaaaac ggtcaggacg ctcgcccctt gccgcggcat tgtactcgtc gagcgtattc
     3001 acgagcgtct cgcgcggaac atcgagcatc ttggcgagcg catcgaggtc cttggccttc
     3061 ttgcagttga aatacaggtt catgagggca gctgcccttt gaaaccattg caacttgccg
     3121 ggcttcgatt gctgccgcgc acgcttcctg agctcctcgt cgatgatcag ggtcgcgatg
     3181 ccgtggttct cttcgaccat cgcctcgccg atcttcgcgc cgtaccagag ctcgtttgca
     3241 aatcgctcgc cattgcgatt gatcagcaac ccttcggcaa atgcgttcgg cgggtagagg
     3301 aaacgccagg ctgaggcgcg tttcatgtgc tctaccttgc cgcccacgct ctgaccgagt
     3361 cgaatcccca tgccgtcatc gcccgcggtg cccagcggca ggcccggcat gaactctggc
     3421 aggtgctcag ccgccatctc ccgattgaaa atgaatccac cggcgcagag gacgacgcca
     3481 cgtttgcatc gaacccgacg gggctccgaa tggtgctcct cgatcgcgtc gcatcgcttt
     3541 cgcagccagg cggcgatccc tggcgcaacc tttgcgatgg cggtttcgag cgtgtgcagg
     3601 tgtcgatgta ccgcgcgcca atagaccgac cggatctgcc ggtattcggc tcccacgacg
     3661 cttcccgctg ggtcgacgat gagtcgtgtc actcgcgcct gaagctcgat gcgcacgcca
     3721 tctgtaatgg ccgttcttcg aaggggttcg atgaggttcg cgccaggaaa cgcaccgccg
     3781 tcgacccggt ggccccgttg tgctggcgtg gccttctcat cgtacgggga gaggggctcg
     3841 ttccccgagt agtacagatg atattcgttg gtcggatacg aggtcttgac cgggcagaga
     3901 ttcgccaaaa atggcacgcc tctgtccgcc agccacttca agttggcagc gctggtctcg
     3961 cagaagtcgc gaagagtttc gtccgacacc gcgtcttgca cctccatcga taggtagcgg
     4021 tacatgttgt cgacgctgtc ctggacaccg gcttcggcct ggatgtgcgt gcctccgccg
     4081 gcgtagaaga caccgccact gatcgacgtg gcgccgccac ccccgaatct gtcgaggaga
     4141 aggacttcgg cgccgcgttc gcgtgcctcg atggcggtgg tgatgcccgc agcgcccaat
     4201 cccaccacga gcaggtccgt ggagtcgtcc cattgcgcgc tgtccgcgtt ctctacgacg
     4261 attggcgtca gcttgctcat tttgacagcg gacgctagca cgcggcctcg aggtctgcag
     4321 cggatcatgg agcttcccac ccatccaaac caagtcggcc ccgtctgcct cgtcacgggt
     4381 ggcaacggct acgtgggcaa gcacctagtt cgccgactgc tcgagctcgg atgtgaagtt
     4441 cgagccttgg atctcgcgcc tttcgtgggc gacgcgcgcg ttcagagcat cgtgggggac
     4501 atacggcgaa tcagcgatgt gcttccggct tgccaaggcg ttgatacggt gtttcacacc
     4561 gccgcgatca tcaacacgct cactctcgcg cgcccgagcg ttcgccgtga ggtctacggc
     4621 gtcaacgtgc acgggaccga agtcgtgatt cgtgcttgca ggaccgcggg ggtaaagaag
     4681 ctcatcttca cgagctcgat cacggtggcg gtcgacggac cgatccgtga aggcacggag
     4741 tcggcgccct acgttggcag cgatggtctg atggatctct attcccagac caagagccga
     4801 gcggagaagc tcgtcttgga agccgatgat ccaaatggcc tgcgcaccgc tgccgttcga
     4861 ccaggtggcg tttgggggcc cggtgagggt gcgatgatgg tcagggattt cctcgagcat
     4921 ctcgcagccg gacagttcaa ggcgacgatc ggagacggtt cgtcagtgac cgacaacgtt
     4981 cacgtcgatt ccgtggtcga tgttcacttc ctggtggctc acaagctcgg tgaggaccca
     5041 aacttggttg gcggagaggc ctatttcgtg agcgatgacg agcccatcaa tccggtcgcc
     5101 tggtaccgcc caatcgtcga agcgctgggg tacacttggc cgaaggtccg gatcccagaa
     5161 ccggtggcgt acgccttcgc ttatctcggg gagctggcgc actatttcgg gggaccgttt
     5221 cccactctca cgcgccgagg agtgctctcg gtctgccgag atgcctcatt tcgggtggac
     5281 aaggctcgcg cacatctcgg ctacgagccg cgaaccagcg cagccgaggg cattcccgag
     5341 ctgatgcagg agtttcgcac gacgcacgac cgcatgaagg ctgcgcaatg atcaagctca
     5401 tctatctgct ctggccgcgt gagccgatgg agcccgcgga ccggcgtgtc atcctgctcg
     5461 aacgctgcgc tccgcttctt ctccagtcag gtgcgcgcta tcttcaaatg aatatcgacg
     5521 acgatctggc gaaagtgcca tcgccggcgc cgacgacgaa gctcaccgat cctttcgtcg
     5581 cggagatcag catctgggtc gaggacatgg ccatgcaaag cgcgctcgag accgtcctgc
     5641 tcgatgcgag cttccacatc gccggctatc gggtacgcga gcacatttac accgactacg
     5701 gtggaaacga gcacggcgcg gcgcgtaact ggcccgatgg ctcgcggtca ccttacgtca
     5761 tctcggtcac gcccttggag cgtccgaagc gaatcccaaa ggataggtgg atgcgccact
     5821 ggttcaatcg ccaggggccc atgtcggaga agatgcagcc tcggagtcgc tacgttcgca
     5881 acgtggtcga cgaggtgctc acacctggcg cgacagcgta cgagggaatc gtcgaggaat
     5941 cctggccttc caagacgcac atgaccaatc cgtttctgtt ctacggggcg cgcaatcggt
     6001 tcgagctggt caagaacatg gcgatcatga tgcagagcgt cgctgcgttc cttcccatct
     6061 ggaagattac gagtgtgacg atgagcgagt acttcgttcg cactccgcgc cgctgagctc
     6121 tcttttcttc tagacgtatc gctgcgattt gtgggtacac tcctcgtccc atgcacaccg
     6181 accgaagagg ctttctcaag atcacgttcg cgggcgcggc tgcgctgtcc acgatgagcg
     6241 ctggtgcgac tttgtcgggt tgcacctcga gcgcgccagc aaatggaatg aagctgcttc
     6301 gccccgagga cctagagctc ctgcgcgcgc tcacgccggc cgtcatgaag gggaagatcg
     6361 cgcctggcga cagcgcggtg atcgagcaaa cgcttcgatc tttcgacacg ctgctggtcg
     6421 atctttcgga gccggtcgtt gcaggcgtgc tccaggcttt cgatgttctc aacttcgctc
     6481 cgactcgagg actgacgacc ggacagtggt cctcctggac gaaggcctct ttgagcgacg
     6541 cggaaagcgc cctcgctcgc cttcgcgaca gcggcatcgg cctcttgaac gcgatctacg
     6601 ccgcagtgat caggctgatc gcgagctctt actacctggt ccccgagaat gcgatcagca
     6661 cgggctaccc cggtccgccg gcgaaggttg caggcgcggc gccgagcata gctccttccg
     6721 gggaggtgac gccgtgagga atctatcgac agtgacgctt ggaagcagca ccgatcccta
     6781 taacaaggcg gaggaacgag gctgggacat caccgatgcg agccagctca gctcagcact
     6841 cgagctcgaa gccgacgtcg tcatcgtagg aaccggcgca ggcggtggca ccgctgcgga
     6901 gattctcagc aaagcgggtc ttcgcgtcgt cattctcgaa gccggtccgc tcaagacctc
     6961 gagccagttc gacatgcaag agcgagctgc ctaccgcgat ctctaccagg agggtactgg
     7021 gcgcggcacc aaagacggcg gcatggtgat tttgcaaggc cgttcggtgg gcggctcgac
     7081 caccgtcaat tggacgacga gctttcgtac gcctcccgaa actctgaagt tctgggcaga
     7141 ggaaatgggg gtcagcggct gctcggtcga agagatggcg ccgtatttcg cccacatgga
     7201 agagcgcctc aacatccatg tatggcctca gccacccaac gccaacaacg ccatgctgca
     7261 gcgcgcgtcg gagaagctcg gttattcctg ggacacgatt caccgcaacg tccgaggctg
     7321 cctgaacctc ggatactgcg ggctcggttg ccccgtcgac gcgaagcaat cgatgctggt
     7381 gacgaccatc ccggcggccc tcgatttgaa ggcgaagctc attcaccgcg cgcgggtcgt
     7441 tcgcgttctg cgtgaaggcg acgtcgtcaa gggtgtcgag gccgaagccc tcacgtcaga
     7501 cgttcgagga acgaccggac agaagatcac ggtccgtgcc gtgcgcaccg ttttggccgg
     7561 tggcgcgatc aacacgcccg cgatcttgtt gcgctccgag gttcccgatc cgcacggacg
     7621 cattggaaag cggaccttcc ttcatccgac gacgctgacg atggggatct atgatgagtc
     7681 gatcggccct ttctacgggg cgccgcaatc gatctattcc gaccactttc agtggcagag
     7741 cgtgagcacg ggtccggtcg gtttcaagct cgaggtcccg ccgttacagc ccgcgtttgc
     7801 ggcagggttc tactcggcgg ccggcgagga gctcacgaag agcatggacc agctcgacca
     7861 cacccaatgc atgatcgctc tcatgcggga cggcttccac gaacagagtc aggggggcgc
     7921 cgtcgagatc aacgacaagg ggcagcctgt gctggactat ccgatcaacg agtaccttct
     7981 cgaaggcgtc cgccgggcct ggaacgtgat gctcgagatg cagtttgcca cgggcgccaa
     8041 gtccgcgagg ccggcacaca agcaggccac ccactaccga tcgtggaacg aagccaaggc
     8101 cgcggttgcc gagctcgaat ttgccgccca caaggtgacc atcgggagtg cgcacgtcat
     8161 gggcggttgt ccgatggggg acgatcccga gcgaagcgtc accgatgcgg cgggcaagtt
     8221 tcaccagctc gagaacctct acatcatgga tggctctctt ttcccgacca gcatcggcgc
     8281 caatccccag ctctcgatct acggtctcgt ctacaagctc tcgagcaacc tcgccgcgca
     8341 attccgcgcc tgatgaacga gaccgttcgc ttcgtcagtc gagggtcgcc aagaaccgga
     8401 gcagactctg gtttacggct tcgggttgct cctgctgagc gaagtgaccg gcgtgaggga
     8461 tgagctgcac gccacggagg tccgggactc ggttcggcat ggtttcgagg gtcttggggg
     8521 tgatgatctg gaggacaggg tctctctccc cgcagacgaa aagagccggc tgtttgatgg
     8581 ggctggttcc atagtcggga tcctgcgccc agttgacgtc catggcgcgg taggaattga
     8641 gaccgccgat gaaagcggtt tcgggtccgg ttcgcatgta ctccgcgacc aaatagtcca
     8701 ggtcttcttc acttagccag ggccagggta agggctcgcc cggctcggcc agcacgttca
     8761 gatagcccgt gccctcggac gggaatttct tgaaatcgag aaggctgcct cgcgcgctga
     8821 gcgcccagta gatgcgcttc aagaatagac gcggattggc gccgagctcg gcctcggcag
     8881 ggccgcgctc ttggaagtag tgcaggtgaa ggaagtggtc cttcgcgatc tcggcgtagg
     8941 tatcgcttgg cttgtgtccc cgcgtccgcg tcccggaggg tctcgttggg tctttcgagt
     9001 cctttggccg acgtcgcgcg aagccgacgc cgtacggcac tgcgatcgaa accagccccc
     9061 tcacacgctc gggtgcgtgc agcgccgcgg cccatgcggc ctgtgcgccg aaatcgtgcc
     9121 cgacgaacac cgcctggtct tcgccgagcg cgtcgagcac cccggtcaga tccgcgacga
     9181 tcgtctggtt gccgtactcg ctcgcgtgcg ccggccggtc gcttcggcca tagccgcgca
     9241 tgtcgagcgc gatcgctcgg tagcctgcgt ctgcgactac cggcagctgg tgtcgccacg
     9301 agtaccagag ccctgggaag ccatggcaga agaggatcgc tggcccttca ccctgggtca
     9361 ccgcatgcaa ccgaatcccg ttggattcga tctgccgatg ctctgcgtcg ctcacgagtg
     9421 ctgcgtgccg cgtactacgg cttcgcagcc tccgccaccg ccgccaccaa gctcttcgag
     9481 aacatgcggc cggtcaggtt gtcgcggacc ttcttgtcgg cggcatccca ttcgggcttt
     9541 gcgtcgccgg cttctaccgc ttggatgccc ttcttcaaga cgatttggta tgccttttca
     9601 tcatctttgc gaatcgtctt gttgagaagc tcgtgcgcac gggctgccgt actgttgaag
     9661 gcttcctgca attccggtgg gagctcgtcg tacttgtcct ggcgcatgac ggtgccgccg
     9721 gcgatgatcg cggagttgtg cgcggtcatg tacttcacgc gtgtgtacca ctgaagcgcc
     9781 accgcggtga gcgccgagga agagatgacg tcgaccattc gggtctgaag cgccgggtag
     9841 acctcgggaa caccgaggcg cacggcgctt gcgccgatga cctggtaaaa ttcgacgaag
     9901 atcggatcgt ctttccagac ccagggccgc atggatttga tgtcggacgg gcgcttgatc
     9961 ggctgcacgg aaaaaagccg tgtcttgccg gcgtcacccc agccgaccag cctcatgccc
    10021 tccttgctga acatgccctc gaactccgtg gccatcttgt cacggacacg gtcgagctct
    10081 tcgtaggtat cgaggaaacc cggaagcgtg aggacgttca tcgcagggac cagcatgctc
    10141 atgcccgtca tcgtgacgac accgccgtcc aactgtccga cccgcatctt gcgaacgaag
    10201 tcgcgctcgt caccctgcga gccgcccggg tagaagcgca tcttgacctt tccgtcggtc
    10261 ttctcctgga gcgtggtgtt ccaagcgttg aggatcttca tccaagaaga acccgcgggc
    10321 gcgagggaag cgatacgcag cgtgtaggct gcttctgcct tggttggcgt gttggtgccc
    10381 ggcagcgcga gtgccgcggc cacgagtagc aagatcggta aaagacgacg attcattctc
    10441 taaaaccttt ccctggcgag ggagcccgcc gagagtcaca agtaacgcgg cgcgcgtcaa
    10501 tatgaatggt ttgctttctg cagcatgcag gacgcttctt cttggaatag ctcgacgagg
    10561 cgagcggggt gtttactcac cgcctcgtcc atcctgaccc cgacgatcac ctttccggcg
    10621 tagcccacga tgcccaggcc gaggtatgct attcccccgt ccgatggtcg gaggtctcgt
    10681 cgagaaccga gagagcaccg cgcgcgatca cctcgcgaat gagcggactt ttctcgcctg
    10741 ggttcggacg gcgctcggcc tggtcggtct cggtgtccta ctcgaacgtc tagtcgccga
    10801 cggcggcgcg attgcggtcg ccgcaggcat aggcttcatc ggtttcggag ggttgtcgct
    10861 gatttacgcg gtgagccgtt atatccgtgt cgcaaagaat ttggagcgag ggatgtttcc
    10921 cgttgcggtg cgggggcctc tcgtgatcgc tttcggtgcc ttgatcgtca cggccggagc
    10981 gatgctctac gtgctgctgc tgaggtgacg ctggcaccgt gcagatgagg ccaaggccag
    11041 gtctggcgct cagaactccg ccaccttgcc acattgaagc atttcgctgc ccatgtacgg
    11101 gttcgacacc ttttcgtcag cctgcaccca caggctgtag ccctctgcca tcgggcagtc
    11161 gtacacccac aaaccctctt ggagacccgg ctctgcggtc aggagggcga ttagctcgcg
    11221 gctcacttcg ccgaacgccg acctggcgcg gcgcaagtcg gttccatcga gctccgcgag
    11281 acgctgcgac gcgccagata gctcttcgag cggcagtcgg agcccttcgg gcgccgattc
    11341 catgccggac cgtgctgcgc tcgccagttc gagcgcttcc gttcttacgt cgctctggtc
    11401 cttggcgagg gctccgcgca cgacttcgta ggcggagagc acgtcattga tgtttgtgag
    11461 ggtggcggcc ggaagggagc tctccggtag aggcgccttc tggctcttgc tgcatcccaa
    11521 gagcgaggcg gtgcagatcg tcacccatgc gagcagaact atcggtcgag cgttcatcat
    11581 cgaatccctc cattctcagc cacatcatcg agctgcaaag tcgaaagaga gccccgcttc
    11641 cagatcgaga aaatcgcagg gtagaccgtg agctcgagaa agaacgacgt cagtaggcca
    11701 ccgaccatag gagcggcgat gcgcttcatc acatccgctc ctgtgcccgt gctccaaagg
    11761 atgggcagga gcgcaatcgt agtggtcatg accgtcatca gttttggccg aatccgcctc
    11821 gcggctccat cgacgatcgc ctccgtgagg tcgtcgggac tccggagctt cccctcctcg
    11881 ctccacttcc ggtgggcgag ggtcaggtag agcagcatga tgactcctgt ttctgcatcg
    11941 agcccggcca gcgcgatcat cccgacccat actgccacac tcatgttgta gtcgagtaag
    12001 aagaggagcc ataccgcgcc gatcaacgag aaggggacgg ccagcatgac gatccaggtt
    12061 tcggtccagg acttcgtgtt ccaatagagg agcagcagca ccaagagcag cgtcacgggt
    12121 acgaccagct tcaatttttc cttggcgcgc tcgagatact tgaactgccc ggcccagccc
    12181 aaccataccc ctgccggcag ctcgacttgt tcgcttacgg cccgcttcgc gtcacgaacg
    12241 tactccgcta tgggccggtc cgtgtccacg aaaacgaagc ccacgagctt gccgtcttca
    12301 ctgcgaacca ttggcggacc ggtcacgaag tcgagggtgg caacctggct gatgggaact
    12361 tgcgcgcccg tcttcgtgct gatgaggatg ttgctgagaa gagacggatt gtcgcggaat
    12421 tctcgggcgt accggaccgt gaccggatag cgttcgcggc cttcgacggt ttcggagacc
    12481 ttcattccgc cgatggcggt catcaccacc tcattgatgt cagcgacccg aagcccgtag
    12541 cgcgcggcct tgtcccggtc gactttgaaa tcgatgaaga agccgcccac cgagcgctcg
    12601 gcaaacgcgc tgcgagttcc agggatcttg gagacggctg cttcgatttg caccgctgtt
    12661 cgctcgatcg tctcgaggtc attgccgaat acctggattc ccagagggct tcgaatcccc
    12721 gttgccagca tctccgtgcg tgtctggacc ggcatccaga agatgttggg catgccgggg
    12781 tagcgcaggg tttggtccat ctccttgatc aagtcatccc aagtgagacc cggcctccat
    12841 tcggatcgag gcttcagcac gatcgtggtc tcgaacattc cgatcggtgc cgggtccgtt
    12901 gcggtttctg ctcgacccgc ctttccaaat accgaaacca cttcgggaaa cgctttcagc
    12961 tcgcggtcca tggcttgaag cacgttggca gcctcggtga ccgacatgcc gggaggcgcc
    13021 gtcggcatat agaggatggt tccctcgttc aatggaggca tgaactcgct ctgcagcgcg
    13081 aggaatgcgg gaatcgtcac cgccatggcg acgaccgcga aggcgatcac gcccctcgca
    13141 tgcgcgacca cgaagcggac gaccggtgcg taggcggaga tgagccaacg attgatcgga
    13201 ttgtcctctt cgtgtcgaat cttttcacgg atcagcatga cggcgagggc cgggatcaga
    13261 gtgacggaaa ggatcgcagc aaagcccatc gaataggtct tggtgaacgc gagtggcttg
    13321 aagagacgcc cttccgcgct ttcgagcgtg aagacgggca ggaacgacac cgtaatcacc
    13381 aagagcgaga agaagagcga cgggccgacc tcctgcatgg ctctgacgat cacctcagac
    13441 cggggtcctt gctgaccccc gtcatcccag ttttcgagct ttttgtggat gttctcgatc
    13501 acgatgatcg acgcgtccac catagcgccg atcgcgaccg caatgcctcc gagcgacatg
    13561 atgttcgcgg tgagtccttg gtagaacatc gggatgaacg acagcatcac cgcaagcggc
    13621 agggtgatga tgggaacgag ggcgcttctc gcgtgcaaga ggaacaagaa gatgatgacg
    13681 ctcaccacga ccatttcctc gatcaaggtg tttcggagcg tgccgatggc tctcttgatg
    13741 agctccgagc ggtcgtacgt gatgaccaac tcgacaccat cgggaaggct ggccccgagc
    13801 tcatcgagct tctgctggat tccatcgatc acggagaggg cgttttcgcc gtatcgcatc
    13861 acgacgat
//
LOCUS       DASRZF010000134        17457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4218215, whole genome shotgun sequence.
ACCESSION   DASRZF010000134 DASRZF010000000
VERSION     DASRZF010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 17457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17457
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4218215"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..2024)
                     /gene="asnB"
                     /locus_tag="VFG22_06140"
     CDS             complement(150..2024)
                     /gene="asnB"
                     /locus_tag="VFG22_06140"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HET6415855.1"
                     /translation="MCGIAGFLTLEPDSAPDADVVRRMCDAMVHRGPDDHGYLTDGPC
                     SLGHRRLSIIDLRPEGAQPMTNEDGSIAAIVNGEIYNFQELRRELVAKGHHFKSRTDS
                     EVLVHLYEEEGPSFLDRLRGMFALALWDRPRRRLLLARDRFGKKPLFYHAGSKGLTFA
                     SELAALASSGHFERRANIDAIDEFLCLQYVPSPSTAFEGVHKLPPGCRLICENGRIGE
                     PERYFELRFDETVTGSLGELSERLHQHIDEAVRLRMVSDVPLGAFLSGGIDSSLIVAM
                     MAKRSSRPVKTFAVGFTSKDFSELPYARMVAERYGTEHHEIVLQPEMTSVIPELVRHY
                     GEPFADSSSLPTWYLCQYTRSGVTVALSGDGGDEAFAGYRRYGHARTARLLRQLPRPL
                     PSLLATTIGNVPTPFAQQVRGFGRRLMQPEHVRFLGLAAHIPQEDRMALYTASMRERY
                     ADDRIALRFGRLYAASTASDPVNRLLDLDIQTYLTDDILTKVDIASMAHSLEVRCPLI
                     DQDLMVFAASLPGRLKLRGLTTKRILREVAKPLLPEKILTRPKHGFRLPLDRWIREDL
                     AEMSRDLLLDRTARQRGIFEPAAIESLLRRHRRGEPLGDQIWALMMLELWYRELIDAC
                     "
     gene            complement(2029..4392)
                     /locus_tag="VFG22_06145"
     CDS             complement(2029..4392)
                     /locus_tag="VFG22_06145"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET6415856.1"
                     /translation="MTRSALILALALGLASFAVWTARRLQLDTGLEALLPDDSVSVLS
                     IEEARDKLGLEEPLTILIASDDADRNKALAGQLAETFRQWPETVWVMTSYGLDALAER
                     ALYYVDADTLYDWSELAEEALDWEVCKASPLCVTIADPPKLPDGDEVRAAIDRSPAGT
                     VLRELTGQTAKQAGTHDEDEAAAQALCNDDGSVCAVQVMLSGSPGDLGYARMIKERVE
                     AITVPLAASQPGETRIEVIGRYRVAPLEHGIIVNDLRLVSILAALGTLLVVLLFFRDF
                     IAVIQLMVPMLSGLAVAVGLITWIEPKINIISASALAILAGMAIDFGIHLLMHYQSAR
                     ADGLSPAASAKESVQELWVSLLVAGLTTACGFGALSMTDFQGFSQMGWMASLGIMVTL
                     LWTLAVFPSMVRLVPGSISAAQQPAAKNRSARGLAWAILLLGVLAIPLALQVGFESNL
                     NKLQPEGVGHGIDASVMRARGNINALYIGRSKEAVNAALDDDLMTRGALVALGVEPVV
                     VSAQVIFPEDADAKTEILENLRSTMDRARAKAIEREDQELLDELDELEPWVDVDGPPQ
                     PDQIPEWLKATLVLKDGSVGMSGIIYVPLRGSNAEAMERLSAWLDQLRKAHPDVTFAS
                     AVALLGEVTPALIADSPWILALVFLGLILATGLASRSFSVTRDVFLATALSTVLFATT
                     LSLFGIDLHLYNLLAVPVVVGLAVDGAVHVRWALDFGNTRQQYATYKAVAASTLTSMV
                     AFGALMTADSPGLRSLGEVGVIGLGISLFVNLVWVRAWCPPAQTQEA"
     gene            complement(4389..5264)
                     /locus_tag="VFG22_06150"
     CDS             complement(4389..5264)
                     /locus_tag="VFG22_06150"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET6415857.1"
                     /translation="MSFRNAIFAGGGSRCFWQLGFWEGAKEAGLDLQDSVKFVGSTSA
                     GCAMASAAVLNRSHDALALFQELTARNPKNIHWHNISPFKPGALLPHASMYREALARF
                     IGPKDFERIRGTSVHFLMSGYPAWLSGAMGAAMALCIYGLEKTLRQDPLHPKWPAKAG
                     FTPLVGRAADCRTAEEYIDMVLAASCVPPVLPEGRFGDRNVLDGGLIENIPVRLADDE
                     PGETLVLLSRKYERPLPSSARVTWVQPSAPIKIDKFDYANPGGLQETFELGIADGERF
                     GRSRVLDQQVDGSAQ"
     gene            5388..5987
                     /gene="thrH"
                     /locus_tag="VFG22_06155"
     CDS             5388..5987
                     /gene="thrH"
                     /locus_tag="VFG22_06155"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF010109.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HET6415858.1"
                     /translation="MPPLVVCLDLEGVLIPEVWIAFAEKTGIDALRRTTRDEPDYDKL
                     MRGRLEILDEHGFKLSDIDDVISTMEPLPGAKEFLDELRAQTQLIILSDTFYQFAAPF
                     MRQLGQPTLFCHELITDENQRVTDYRLRVADGKRKAVAALKSIGFRVHAAGDSYNDTT
                     MLKEADRGILFRCPDNVAAEFPQFQRTDAYSELLPALLR"
     gene            complement(6000..6923)
                     /locus_tag="VFG22_06160"
     CDS             complement(6000..6923)
                     /locus_tag="VFG22_06160"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6415859.1"
                     /translation="MRRMNAINATLLAAAVLLGGALTAAVSARTPAEHADDGARSVVD
                     ATGARVPIRAYARIVSASTIADQVLIEIIEPRRVLAVSRHTLRTQKSPAYRDKIGVEH
                     ARDIETIIELRPDIVFINNFVDRRHVARLKEAGLNVFDLGAMRGLETLLPNIQQLAEV
                     LDVREKGQALALDLVDRLRKVSAEVPIAERQRGLYVGIHGDRLYGGAEGTSFHDVLVA
                     AGLVDVASEAGFTGWPAFTSEQLLSLDPPWIITNPGTERSLCRHAGLGSLSACRLGQV
                     RSIETDLLTDPGLGILRAAQAVHAVVYGDAS"
     gene            complement(6925..7722)
                     /locus_tag="VFG22_06165"
     CDS             complement(6925..7722)
                     /locus_tag="VFG22_06165"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6415860.1"
                     /translation="MRSGEEPITLRTRGLSVKLGGRTVLEGIDLDAKPGEVLALLGPN
                     GAGKSTLLKALAGLLPYEGRVEIADFDLASLSPRKRAKRISYVPQRSLLRSALSVDEV
                     VALGRYVHGSNFGSMSKNDKEAIDHALETTHADVLRGRIFTRLSVGEQQRVLLARALA
                     SDAPIVLLDEPTAALDVGEAMAVLRLIRKLAARKHTLIVVLHDLADARSTTDRALLLK
                     KGRVVEQGKARDVISPDPIRAVYGVRLIENARIGFESETETETVGTK"
     gene            complement(7715..8689)
                     /locus_tag="VFG22_06170"
     CDS             complement(7715..8689)
                     /locus_tag="VFG22_06170"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET6415861.1"
                     /translation="MRLYPLLILLLIVAGALAVLVGSGDLSDPELRDTFLKLRGYRLA
                     AAFLAGAALAVGGVVVQGLFRNPLASPSVLGTTAGASFGGQVALLAYGVAGVGTSLVV
                     PEMIVPIGCFIGALAALAILLLFLRITEDLLALLLTGFVLSALFLSLSAFVTSIAQET
                     WELGRAVVSFSLGSVSGTGPRQLAFALPLVMTALLASWFWGRSLDLLLSGEEEAQAMG
                     ANVGTVRWWSAIWVSVLVAGAVSVGGNVGFVGLVVPHMLRPFTGTKHQRLIPAAALAG
                     GTFLVACDIIARVIPSRSEIPLGVVTGLIGAPLFLLLLVRLRRERAYA"
     regulatory      8731..8923
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            9002..11038
                     /locus_tag="VFG22_06175"
     CDS             9002..11038
                     /locus_tag="VFG22_06175"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET6415862.1"
                     /translation="MDQNQQVRSKRWGRQICRALGLVAVCAGAALPSFPALSQFGETA
                     EVTRPIPSTNSLDATAAGSEIVIRDRFVAQSTYQLLQESAGTRVVSEGGVGTPFCLRL
                     RGVACDQVTVMLGEVPISSPDTGSFDLSLVPLEALDGFEVFRGGSPSWLNNGAVGGVL
                     RLLPRTYKETEVGGRVTGGSYGTWRANLFGAASSKKVQFFGTAGGAGARNDYPYLDDG
                     GTRFDPSDDVERQRQNADFKEGFGFANMRARTSKTSHLDLVFLGLGRERGEPGPGSSP
                     VLQVRVEQTRLIGSLSWLQEKDGAHPYRLQVAINYDYGRNRTDDQFGELGRGGPLRSN
                     DQTHAVFGRVAASVKAMPWLEVTTIGTARYQVFDPDEELSSAPEPASDRLTATGTVET
                     RFFGDVGKVGLELRPSVRLSWTRAAIREAPLVGQAPGESSELLPTYRVAGAISPLEWM
                     AFRGSISSGYKLPSLLQLFGNRTSVEGNPSLVQERSLAYDGAVTLRGHTGAVSGYVAA
                     GAFLTHLDDAIRFRRTSPSKVKAENIGAARIRGVEIELRGGVTQHFIVQGEVTWTEAT
                     DQSSGNQLPAQPEWVAFVQPEAHSGTLSKSLSDIMAFFQISYLGKTYDDPANLVEVSA
                     RTVLATGVGVLMFEGRLGLSFRVDDILDVRGQDLVGYPLPGRRYTGRLSYRHAW"
     regulatory      11111..11291
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            11389..12717
                     /locus_tag="VFG22_06180"
     CDS             11389..12717
                     /locus_tag="VFG22_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415863.1"
                     /translation="MEREFRIILLICAALVMLGCGADGTDGDGGSAGTGGAAGMLGTP
                     ALDPLDAAPRFAVVSTDFSSSSIAMLDEDFVVIDESWLSSGTTYPGLVASLSGDVVLP
                     NRQAGDGTFAVIDRFFTDVVTRFFSPSGNLDGQVRTHGEVGDSGFSSNPQDFIFVDAD
                     SAWVPRYESNLNPAASPESRGNDLIEINPSDMSATGARIDLSTLDTSATVMAGEGSAE
                     VDVFARPSRGVLFGSTIVVGLDRISAGFEAAGSGMVAVVDLEDDSIVGLELSGLASCG
                     NAVPVPEAPNKVLVACVGFAQPFGDEPQVRASAGLVLLEVGASGVQIERSWRLADHPS
                     AAIAVNSVIAIDDRRALGVATGNFVDTVDQLYLIDLVTGDQELVLESGDSFVIGLSAY
                     DPDSRILYVPDAFENAVIELGADDDGFVELGSTEIAPGLGLPPSKVYLLD"
     gene            complement(12769..13224)
                     /gene="arfB"
                     /locus_tag="VFG22_06185"
     CDS             complement(12769..13224)
                     /gene="arfB"
                     /locus_tag="VFG22_06185"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HET6415864.1"
                     /translation="MTSEAPATDKDLVINERVTVPAHDLSWTASRASGPGGQNVNKVA
                     TKVTLRFDLRATNALTAPQKARLRKLAANRIDATGVLSLSAQAERSQRQNLTRARDRL
                     RGLIVEALIPPTRRVATKPSRAAKRRRLEEKRRRGDRKKARGKVSPAGD"
     gene            complement(13251..14093)
                     /gene="rfbD"
                     /locus_tag="VFG22_06190"
     CDS             complement(13251..14093)
                     /gene="rfbD"
                     /locus_tag="VFG22_06190"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HET6415865.1"
                     /translation="MNDFVLIGADGMLGRCWAELLTARGIHHVGTTIEDLDITDRANV
                     ARFVKPGTQWVVNCAAYTLVDAAEDYEDLANEINGRAVGRLAERCREVGAKLLHYSTD
                     YVFNGNASEPYPIDHPRDPVNAYGRSKALGEELIEQSGVEHLLVRSSWLYAPWAKNFV
                     LTIRKLAMSRDSLRVVDDQRGRPTDSRRLAEVSLALAELGQRGTFHVADSGECTWFEL
                     AAFIAHEVNPDCRVEPCTSDEFPRPAPRPAYSVLDIRATEEVAGPMMPWKDRVRDVLA
                     RVED"
     gene            complement(14086..14640)
                     /gene="rfbC"
                     /locus_tag="VFG22_06195"
     CDS             complement(14086..14640)
                     /gene="rfbC"
                     /locus_tag="VFG22_06195"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HET6415866.1"
                     /translation="MKVAAARIPGLLVVEPDVHGDERGFFMESYSRDRYVEAGLPGEF
                     VQDNLSLSARGILRGLHLQHPKAQGKLCFVLAGEVFDVAVDVRVGSPTFGSWEGLTLS
                     AENKRQFYVPPGFAHGFCVLSEQALFSYKCTEFYSAPSELGIAWDDPDIGIAWPIQSP
                     KLSRKDQQNFRLEDIPLEQLPRYE"
     gene            complement(14640..15521)
                     /gene="rfbA"
                     /locus_tag="VFG22_06200"
     CDS             complement(14640..15521)
                     /gene="rfbA"
                     /locus_tag="VFG22_06200"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016283109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HET6415867.1"
                     /translation="MIKKGIILAGGTGTRLHPLTKGLSKQLMPIYDKPMIYYPLSTLM
                     LAGIREILVITTPHERALFEGLLGDGESWGLRIEYAVQPSPDGLAQAFLIGERFIDGE
                     ACALVLGDNIFYGHGLQGLLAESARQTEGATVFGYYVNNPTAYGVAEFDEDGVVVGLE
                     EKPKEPKSNYAVAGLYFYDRDVVAHAKALKPSARGELEITDLNRRYLSVGKLRLQKFG
                     RGVAWLDTGTPVALLQAANFVQTIQERQGLRASCPEEISFRMGWIDEEQLERLAKQLA
                     KTEYGEYLHALARGLDI"
     gene            complement(15531..16583)
                     /gene="rfbB"
                     /locus_tag="VFG22_06205"
     CDS             complement(15531..16583)
                     /gene="rfbB"
                     /locus_tag="VFG22_06205"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HET6415868.1"
                     /translation="MKIIVTGGAGFIGANFLNRLVPELPDHQFINLDKLTYAANLGSL
                     RSIDSLPNYGLRRVDITDMNAVDAVFDEVDPDLVVHFAAESHVDRSILGPREFIRTNI
                     EGTFNLLEACRKRWSRDDHHLFHHVSTDEVYGSLGDTGLFEESTSYDPSSPYSASKAA
                     SDHIVRAYHRTYGLPIKITNCSNNYGPMQFPEKLIPLMILNALERKPLPIYGKGLNVR
                     DWLYVSDHCDAIWTVIQKGRVGRTYNVGGNNEMTNIEVVDILCRIMAEETGSDPEELL
                     RLKQFVTDRPGHDLRYAIDASRIREECGWEPKETFDTGIRKTVQWYLANHDWVADVRS
                     GDYRKWMDLNYGDRTE"
     gene            complement(16665..17396)
                     /locus_tag="VFG22_06210"
     CDS             complement(16665..17396)
                     /locus_tag="VFG22_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415869.1"
                     /translation="MKRFAFVLGTILTVAAGASAQDLPGTFSVEQNGVSLTGSGKEVR
                     EDVPEKKKAYNKKVGGIVWLEATAGPSRFDATRFRTLDIVPAGVQQLVPDVSVRGPEF
                     GAALRFRAGSTTIGARYKRADYEPFDLTTVGLDLGFLIRAVPYVHPSIRLGFNYCTTT
                     GGVVIPGFENLVNNVRSNGGGASIGAGLRIPIVRWVSIAADFDYSVIGLVFRGTETVT
                     GGQFTAGTAGTAIAGTFALTFHLGG"
BASE COUNT         3303 a         5419 c         5481 g         3254 t
ORIGIN      
        1 gagccatcct gggaatcgtc gtcgacgaac aggagctcgt agccaccttc gaagtcctct
       61 agggccgagt cgatggcctg agcgagcaag gggagattgt cagcctcccg gtaggtcggg
      121 accacgattg agatcacgcg atggcgtcct tagcacgcat cgatgagctc gcggtaccag
      181 agctcgagca tcatcagcgc ccagatctga tcgccaaggg gctcgccgcg ccgatgccgg
      241 cgaagcaagg attcgatcgc ggccggttcg aagatcccgc gctggcgggc cgtccggtcg
      301 agtaagagat cgcgcgacat ttcggccaaa tcctcccgga tccatcggtc taaaggaagc
      361 ctgaagccgt gcttgggtcg agtcaggatc ttctccggga gcagtggctt agcgacttct
      421 cgcaagatcc gtttcgtcgt cagtccgcgg agcttcagcc gacccggaag ggaggccgca
      481 aagaccatca agtcctggtc gatgagcggg cagcgtacct ccaacgaatg agccatcgat
      541 gcgatatcga ccttggtgag aatgtcgtcg gtgagatagg tctggatgtc caagtcgagg
      601 agacgattga ccggatcgga agccgtggat gccgcataga gtcttccgaa tcgcagcgcg
      661 atgcggtcat ccgcatagcg ttccctcatg gaggctgtgt agagcgccat tcggtcttcc
      721 tgcggaatgt gggcggccag gccgaggaat cgcacgtgct caggctgcat caagcgtcgc
      781 ccgaaaccac gcacttgctg cgcaaagggc gtagggacgt ttccaatcgt cgtcgccagc
      841 aaggacggca ggggtcttgg aagctgacgg agcaggcgag ccgtgcgggc gtgaccgtag
      901 cgacggtaac ccgcaaacgc ttcgtcgccg ccatcgccgc tgagtgcgac cgtgacgccg
      961 ctccgcgtgt attggcagag gtaccaagtc ggaagggacg agctatcggc aaaaggctcg
     1021 ccgtagtgac ggaccagctc gggaattacc gacgtcatct cgggttgaag cacgatctcg
     1081 tggtgctcgg tgccgtatcg ttcggccacc atccgcgcgt agggaagctc gctgaagtcc
     1141 ttgctggtga acccaacggc aaacgtcttc acgggacgcg acgatcgctt agccatcatg
     1201 gcgacgatta gagaagaatc gattcctcct gagaggaacg cgcctagtgg aacgtccgac
     1261 accatgcgga gccgcaccgc ctcgtcgata tgctggtgca gacgttcgct cagctcgccg
     1321 agggatcccg tgaccgtctc gtcgaaccga agctcgaaat agcgctccgg ctcaccgatg
     1381 cgcccgttct cacaaatcag ccggcagccg ggtgggagct tgtgaacgcc ttcaaatgcc
     1441 gtcgagggcg acggcacgta ctgaaggcag aggaactcgt cgatcgcgtc gatgttcgcc
     1501 cggcgctcga agtgccccga cgaagcgagc gcggcgagct cggacgcgaa ggtgagcccc
     1561 ttggaaccag catgatagaa caggggcttc ttgccgaagc gatcgcgcgc gaggagcaag
     1621 cggcggcggg gtcggtccca gagtgcgagc gcaaacattc cgcgcaatcg gtccaggaag
     1681 ctcgggccct cttcttcgta gagatgaacg agcacctcag agtccgttcg cgacttgaag
     1741 tggtgccctt tcgctacgag ctcgcgccgt agctcttgga aattgtagat ctccccgttg
     1801 acgatggccg cgatcgagcc gtcttcattc gtcatcggtt gagcgccttc cgggcgcagg
     1861 tcgatgatcg aaagccgtcg atggccaagc gagcaagggc cgtcggtgag gtatccgtga
     1921 tcgtcaggcc cgcgatggac catcgcatcg cacatccgcc tgacgacgtc cgcgtcgggc
     1981 gccgagtctg gctctagcgt caagaacccg gcgataccgc acatggagtc aagcctcctg
     2041 agtctgcgcg ggcgggcacc aggcgcgaac ccagacgagg ttgacgaata gcgaaatccc
     2101 aaggccgatg acgccgacct ccccgagcga gcgaagaccc ggactgtcgg cggtcatgag
     2161 cgctccgaat gcaaccatcg aggtgagtgt cgaagcagcg acggctttgt aggtcgcata
     2221 ctgctgtcgc gtgttgccaa aatcgagagc ccagcgaacg tgaacggcgc catcgacagc
     2281 gagaccgacg acgaccggca ccgcgagcag attgtagaga tgcagatcga ttccgaagag
     2341 gctcagcgtc gttgcgaaga gaacggtgct cagcgcggtc gctagaaaaa cgtctcgagt
     2401 cactgagaac gagcggcttg cgagaccggt cgccagaatc aatccgagaa acaccagcgc
     2461 aagaatccat ggagagtccg caatgagtgc cggtgtcacc tctccgagga gcgcaaccgc
     2521 tgatgcgaag gtcacgtcgg gatgtgcctt gcggagctga tcgagccagg ccgacagacg
     2581 ctccatcgct tcggcgttcg acccgcgcag tggaacatag atgattccgc tcattccgac
     2641 cgagccatct ttcaagacga gtgtcgcttt gagccactcg ggaatctgat cgggctgcgg
     2701 agggccgtcg acgtcgaccc agggctcgag ctcgtcgagc tcgtcgagaa gctcttgatc
     2761 ctcacgttcg atggccttcg ctcgcgcgcg atccatcgtg ctccggagat tctcgaggat
     2821 ttcggtcttc gcgtccgcgt cctcggggaa gatcacctgc gccgatacga cgaccggttc
     2881 cacgccgaga gcaaccagcg ctcctcgggt catcagatcg tcgtcgagcg ccgcgttcac
     2941 cgcttccttt gatcgaccga tgtagagcgc gttgatgttt ccgcgcgcgc gcatgacgga
     3001 ggcgtcaatt ccgtgaccga cgccctcggg ttggagcttg ttcaggttcg attcgaagcc
     3061 gacctgaagg gccagcggaa tcgccagcac gccgagcagc aggattgccc aggccagacc
     3121 gcgcgcgctc cggttctttg cagccggctg ctgggctgcg ctgatggacc ccgggacgag
     3181 gcgaaccatg ctcgggaata ctgcgagggt ccaaaggagc gtcaccatga tgccgagcga
     3241 agccatccaa cccatttgcg agaagccctg aaaatcggtc atcgacaggg ctccgaagcc
     3301 gcaggccgtc gtgaggcccg ccaccagaag cgacacccag agctcttgga cgctttcctt
     3361 ggcggacgct gcggggctca gaccgtcggc gcgagcggac tggtagtgca tcaagagatg
     3421 aatgccgaag tcgatcgcca tacccgcgag aatggccaat gccgacgcac tgatgatgtt
     3481 gatctttggt tcgatccagg tgatgaggcc gacggccacc gcgagtccgc tcaacatggg
     3541 gaccatgagc tgaatgaccg cgatgaaatc tcgaaagaac aggagcacca cgagcagcgt
     3601 ccccaaagcg gccaggatcg ataccaggcg caagtcgttc acgatgatcc cgtgctcgag
     3661 gggagctact cggtagcggc cgatgacctc gatacgagtt tcgcctggct gcgacgccgc
     3721 cagtggaact gtgatggcct cgacccgctc cttgatcatt ctggcatagc cgagatctcc
     3781 gggtgagcca ctgagcatga cctgcaccgc gcagacggag ccatcgtcgt tgcagagcgc
     3841 ctgcgctgca gcctcgtctt cgtcgtgcgt acccgcttgc ttggcggttt gtccggtgag
     3901 ctcacgaagt acggtccccg ctggcgaccg atcgatcgcc gcccggactt catcgccgtc
     3961 gggtagcttc ggggggtccg cgatcgtcac gcagagcggg gacgctttgc acacttccca
     4021 gtcgagcgct tcctcggcta actcgctcca atcgtagagg gtgtccgcgt cgacgtagta
     4081 gagggcacgc tcggcaagcg cgtccaagcc atagctcgtc atcacccaga ccgtttcggg
     4141 ccactgtcga aaggtctcgg cgagctggcc ggcgagggcc ttgttgcgat ctgcgtcgtc
     4201 ggaggcgata agaatcgtga gcggctcctc gagtccgagc ttgtcccggg cttcctcgat
     4261 ggacagcacc gagaccgagt cgtctggcag gagcgcttcg aggcccgtgt cgagctgtag
     4321 ccgcctcgcc gtccagacag caaacgatgc aagaccaagc gcgagggcga gtatcagtgc
     4381 ggatcgcgtc actgcgcgga accatcgacc tgctgatcga ggacacggct ccgcccgaaa
     4441 cgctcaccgt ctgcgatgcc cagctcaaat gtttcctgca ggccgccggg gttcgcgtag
     4501 tcgaatttgt cgatcttgat cggtgctgag ggctgaaccc aagtgactcg cgcgctcgag
     4561 gggagcgggc gctcgtactt gcggctcaag agaacgagcg tctcgcctgg ctcgtcatcc
     4621 gcgaggcgaa ccgggatgtt ttcgattaga ccgccgtcga gcacattgcg gtcgccaaat
     4681 cggccctccg gcaaaaccgg tggcacacag gacgcggcca agaccatgtc gatgtactcc
     4741 tccgccgttc ggcaatcggc ggcgcgaccg acgagcggcg tgaacccagc cttcgcgggc
     4801 cacttcggat gaagagggtc ctggcggagc gtcttttcga gtccgtagat gcagagagcc
     4861 atcgctgcgc ccatcgcccc gctcagccac gcggggtagc cgctcatcag aaaatggacc
     4921 gaagttccac ggatccgctc gaagtccttg gggccgatga atcgagccag tgcctcgcga
     4981 tacatgctcg catgtggaag caaggccccg ggtttgaaag ggctgatgtt gtgccagtgg
     5041 atgttcttcg ggttcctggc ggtcagctcc tggaatagag ccagtgcatc gtgagagcgg
     5101 ttgagcacgg ccgcgctagc catggcacag cccgcgctcg tcgaaccgac gaacttcacc
     5161 gagtcttgaa gatcgagccc cgcttccttg gctccttccc agaaaccaag ctgccagaaa
     5221 cagcggcttc caccgcccgc gaagattgcg tttctgaacg acatgagcgg cacattgtgg
     5281 acccagcgac gccgaactgt cgaggcaaag cgagttgcct gggctcgaat atcgggtaca
     5341 cgaagcgcct tctcacagct gcccggccgg gcattggagt accgctcatg ccgccactcg
     5401 tcgtctgcct cgatctcgaa ggagtcctca tccccgaggt gtggatcgcc tttgccgaga
     5461 agaccggaat cgatgccctc aggcgcacga cgcgcgacga gccggactac gacaagctga
     5521 tgcgtgggcg gctcgaaatc ctcgacgagc atggtttcaa gctgtccgac atcgacgacg
     5581 tcatcagtac gatggagccc ttgccgggcg ccaaggagtt tctggacgag cttcgcgcgc
     5641 agacgcaact catcattttg agcgacacct tctatcagtt cgctgcgcca ttcatgcgcc
     5701 aactcggcca gccaacgctc ttttgccacg agctgatcac cgacgagaac cagcgcgtga
     5761 ccgattaccg ccttcgcgtt gctgatggca agcggaaggc ggtcgccgcg ctcaagagca
     5821 tcggcttccg cgtgcacgcc gccggtgaca gctacaacga cacgacgatg ttgaaggaag
     5881 ccgaccgcgg aatactgttc cgatgtcccg acaatgtcgc cgcggagttt cctcagttcc
     5941 aacgcaccga cgcctacagc gagttgcttc cggccttgtt gcgctagggc gagtgcgttc
     6001 tacgaagcgt cgccgtagac caccgcatgc accgcttggg cggctcgaag gattcccagg
     6061 ccggggtcgg tcagcagatc ggtctcgatg ctccgcacct gtccgagtct gcaggcggag
     6121 agcgaaccta ggcccgcgtg ccggcaaagc gagcgttcag taccaggatt ggtgatgatc
     6181 catggcggat cgaggctcaa gagctgctcg ctggtgaatg ccggccagcc ggtgaatccc
     6241 gcttcggaag caacatcgac gagcccggcc gcgacgagca catcatgaaa gctcgttcct
     6301 tctgctccgc cgtagagccg gtccccgtga attccgacgt agagaccgcg ctgcctttcg
     6361 gcgataggca cctccgcgga gacctttcgc agtcgatcga ctaggtccaa cgcaagcgcc
     6421 tgccctttct cgcgaacgtc gagcacctcg gccagctgtt ggatgttcgg tagcaaggtc
     6481 tccaagccgc gcatcgcacc gagatcgaac acgttgagcc ctgcttcctt gagccgcgct
     6541 acgtgccggc ggtccacgaa attgttgatg aacacgatat ctggccggag ctcgatgatg
     6601 gtttcgatgt cgcgcgcgtg ctcgacgccg atcttgtctc gatacgcggg cgacttctgg
     6661 gtgcggaggg tgtgtctgct cactgccagc acccgccgcg gctcgatgat ctcgatcaga
     6721 acctggtcgg cgatcgtgct cgcgctcacg atgcgggcat atgcacggat cggtacgcgc
     6781 gcaccggtcg cgtccaccac gctccgagca ccatcgtctg catgctcggc gggcgttctc
     6841 gccgagactg ccgcggtgag ggctccgccg aggagcacgg ctgccgccaa aagggtcgcg
     6901 ttgatcgcat tcatgcgcct catgtcactt cgtacccacc gtctccgtct ccgtctcgga
     6961 ctcgaagccg attcgtgcgt tctcgatgag tcgtactccg tacaccgctc gaatcggatc
     7021 gggactgatc acatcgcgcg ccttcccttg ttcgaccaca cgcccttttt tgagcaagag
     7081 agcacggtcc gtcgtgctcc gcgcatctgc gagatcgtga agcacgacga tcaatgtatg
     7141 cttgcgcgcg gcaagcttcc tgatcaggcg gagcacagcc atcgcctcgc ctacgtccag
     7201 ggctgccgtg ggttcgtcga gcaagacgat gggcgcatcc gatgcaagcg cacgagcaag
     7261 caagacgcgt tgctgctcgc cgaccgagag tcgagtaaaa atccggcccc gcaagacgtc
     7321 tgcgtgcgtc gtctcgaggg catgatcgat cgcctccttg tcgttcttcg acatgctccc
     7381 gaagttgctc ccgtgcacgt atcggcccaa cgcgaccact tcgtctaccg aaagggccga
     7441 acgcaggagg ctccgctgcg ggacgtacga gatgcgtttg gcgcgctttc gcggtgagag
     7501 tgaggccagg tcgaagtcag cgatctcgac acgcccctcg tagggaagaa ggcctgcgag
     7561 cgccttcaag agcgtgctct ttccggcccc attcggaccg agaagggcga gcacctcgcc
     7621 gggcttggcg tcgagatcga tcccctcgag cacggtccgt ccaccgagct tcacgctcaa
     7681 gccgcgggtg cgaagcgtga tcggctcttc tccgctacgc atacgcgcgc tcccgccgca
     7741 gtcggaccaa gagcagaagg aagagcggag cgccgatcaa tccggtcacg acgccgagcg
     7801 gaatttcgct acgtgaagga atgacgcgcg caatgatgtc gcaagcgacg aggaacgtcc
     7861 ctccggccag tgcggcggct ggaatcaatc gctggtgctt cgtccccgtg aagggtctca
     7921 gcatgtgcgg caccacgagg cccacgaagc cgacatttcc gcccacggac actgcgccag
     7981 cgacgaggac gctgacccag atcgccgacc accagcgcac cgtgcccaca ttcgcgccca
     8041 tggcctgagc ttcttcttcg ccgctcagca agagatccag ggaacggccc cagaaccagg
     8101 acgccagcag ggctgtcatc accagtggca aagcaaacgc cagttggcga ggcccggttc
     8161 cgctgaccga gcccagcgag aaagacacca ccgcacgacc gagctcccag gtctcctggg
     8221 cgatgctcgt cacgaacgcg ctgaggctca agaagagcgc gctcaagacg aaccctgtga
     8281 gcagaagcgc tagcaagtcc tccgtgatcc gaaggaagag caagaggatt gccaacgccg
     8341 cgagggcacc gatgaagcag ccgatcggta cgatcatctc cggaacgacc aggctggttc
     8401 cgacgcccgc aaccccatag gcaagaagag cgacctgtcc accgaagctc gcaccagccg
     8461 tcgtaccgag caccgacggg ctcgccagcg gattccgaaa caagccctgc acgaccacac
     8521 cgccgactgc gagcgctgca cccgcgagaa aagctgcggc cagtcgatat ccacgcagct
     8581 tcaagaacgt gtcgcgaagc tccggatccg agagatcgcc cgatccgacg agtacagcga
     8641 gcgcccccgc gacgatcaag agcagaatga gcaacggata caggcgcacc gcgcgtcctt
     8701 accatggccc ccgagcacga tcgacacggt tccgagacga ttgaccctcg cgagcggatc
     8761 agtcatcgtc cgctcaagca gggaagtcgg tgagaagccg gcgcggaccc gccacggtaa
     8821 gtgactgaat gttctgtcgt gatgccactg ggcgctgagc ctgggaaggt gacaggacct
     8881 ggggtcacga gcccggaaac ctgcgaacgt attgttgtgc ttgtcgcctc ggggatggcg
     8941 agagtcgcgc gtcgggattc cgattccgtt cgatcgaccg ttcccgaatt gggaacggga
     9001 attggatcag aaccagcagg tgcgctccaa gcgatgggga cggcagatct gtcgtgcctt
     9061 gggcctggtc gccgtctgcg ccggagctgc gctgccgagt ttcccagctc tttcgcagtt
     9121 tggtgaaacg gcggaggtga ctcgcccgat tccctcgaca aactccttgg atgcgaccgc
     9181 ggcgggaagt gagatcgtca ttcgggaccg tttcgtcgcg cagtcgacct accaactcct
     9241 gcaggagtcg gcaggtacgc gggtcgtttc cgaagggggt gtcggcacac cgttctgtct
     9301 tcgcctccgc ggcgtggcct gcgatcaggt gacggtgatg ctcggggagg ttccgatcag
     9361 cagcccggac accggatcct tcgacttgag cctcgtgcct ttggaagcct tggatggctt
     9421 cgaagtattt cgggggggct caccttcttg gctgaacaac ggcgcggtgg gcggcgtgct
     9481 ccgcttgctg ccgcgcacat acaaggagac cgaggtcggg gggcgcgtca ccggaggcag
     9541 ttacgggacc tggcgtgcga acctcttcgg cgctgcgtcc tcgaagaagg tgcaattctt
     9601 cgggaccgcg ggcggcgccg gcgcgcgcaa cgattaccct tacctcgacg atggcggaac
     9661 ccggttcgat cccagcgacg acgtcgagcg ccagcgccag aacgcggatt tcaaagaagg
     9721 cttcgggttc gccaacatgc gcgcgcggac gagcaagacg agccatctcg atctcgtatt
     9781 cctcggcctc ggcagagagc gaggagagcc cggacctggt tcgagcccgg tgctgcaggt
     9841 gcgcgtcgag caaacacggt tgatcggatc gctttcctgg ttgcaggaaa aggacggcgc
     9901 gcatccctat cgcctccaag tggcgatcaa ctacgactac ggccgaaacc ggaccgacga
     9961 ccagttcggc gagctcggtc gaggcgggcc actgcgcagc aacgaccaga cccacgcggt
    10021 cttcgggcgg gtggcggcgt ccgtcaaggc catgccttgg ctcgaggtga ccacgatcgg
    10081 cacggctcgc taccaagtgt tcgatccgga cgaagagctc tcgagtgcgc ccgagcctgc
    10141 ttccgatcgg ctcactgcga cgggaactgt cgagacgcgg ttcttcgggg atgtcgggaa
    10201 agtcggtctc gagcttcgcc ccagcgtgcg gctgagctgg acacgcgccg cgattcgcga
    10261 ggcaccgctg gtgggtcagg cgccggggga gtcctccgag cttctaccca cgtatcgtgt
    10321 ggctggcgcc atctcacctc tcgagtggat ggcatttcga ggctcgatca gcagcggcta
    10381 caagctcccg tccttgttgc aactcttcgg gaaccgaacg agcgtcgagg ggaatccgag
    10441 cctcgtgcaa gagcgctcgc ttgcgtacga tggcgccgtg acgctccgag gacacaccgg
    10501 cgccgtcagt ggctacgtgg cggccggcgc gtttctcacg cacctcgacg acgcgatccg
    10561 attccggaga acctcgccat cgaaagtcaa agccgagaac atcggtgcgg cccgaattcg
    10621 gggggtcgaa atcgagcttc gcggaggggt cactcagcac ttcatcgttc aaggcgaggt
    10681 cacctggaca gaggcgaccg accagtcgag cggcaatcag ttgcccgccc aacctgagtg
    10741 ggtcgccttc gttcagccgg aagcacactc gggaacccta tcgaagtcgt tgtccgacat
    10801 catggcgttt ttccagatct cgtacctcgg caagacctac gacgatccgg cgaacttggt
    10861 cgaggtgtct gccaggactg tgctcgcaac gggcgtgggc gtgctcatgt ttgaaggtcg
    10921 actcggcctg agctttcgtg tggatgacat tctcgacgtg cgtggccagg acctggtcgg
    10981 ctatccgctt cccggacgcc gctatacagg gcggctgagc tatcggcacg cgtggtagat
    11041 tgactttggt cccgctgacg gtacggttcc gcctcttgcg agggttgaag gcggcttcgt
    11101 cgaagccgct ggagatagat cggcggagag aaatccgctc gattgaacag ggaagtcggt
    11161 gtgacgccga cgcggacccg ccacggtatg tgatcgaatg cgctgccacg acgccactgg
    11221 gtgagaacct gggaaggcgg cagagcctag ggtcacgagc ccggagacct acccatcgtg
    11281 agctcactcg aatcgcctcg gggagggcgg gtggttacca aggatttcgt tcaacccatt
    11341 gaacgcacct cgtttccgtc tgttgtcctc ctggcttgga ggaagccaat ggaacgggaa
    11401 tttcggatca ttcttctaat ctgcgcagcg ctggtcatgc ttggctgtgg cgcagacggc
    11461 acggatgggg acggcggcag tgcagggacc ggcggcgctg cgggcatgct cggcacccca
    11521 gcgctcgacc cgctcgacgc ggcccctcgt ttcgcggtcg tcagcacgga cttcagcagc
    11581 tcatcgatcg caatgctgga cgaggacttc gtcgtcatcg acgagagctg gctgagctcg
    11641 ggcaccacgt accccggcct cgttgcaagc ctctccggtg acgtggtgct gcccaacaga
    11701 caggcgggcg acggaacgtt tgccgtcatc gatcggttct tcacggacgt cgtgacgcgc
    11761 ttcttctcgc cgagcggcaa tctcgacggg caggtgcgaa cccatggtga agtcggcgat
    11821 tcgggtttct cttcgaaccc gcaggatttc atcttcgtcg atgccgacag cgcgtgggtt
    11881 ccgcgatacg agtccaatct caaccccgcg gcgtcgccgg agagccgggg aaatgacctc
    11941 atcgagatca acccttccga catgagcgct acgggcgctc gaatcgatct cagcacactc
    12001 gatacgtcag ctactgtcat ggctggcgaa ggctcggcgg aagtggatgt gttcgcgcgt
    12061 cctagccgtg gggttctctt cggctccacg attgtggtgg gcttggatcg aattagcgcc
    12121 gggttcgaag cggccggctc cggcatggtc gcggtcgtcg atctcgagga cgactcgatc
    12181 gtcggtttgg agttgagcgg cctcgcgagt tgcgggaatg ctgtcccggt tcccgaggcc
    12241 ccaaacaagg tattggtggc ctgcgtagga ttcgcgcagc cgttcggcga cgaacctcaa
    12301 gtgcgcgcct cggcgggcct cgtcctgctc gaggtgggcg cctccggggt ccagatcgag
    12361 cggagctggc ggctggcaga ccatccaagc gcggcaattg cggtgaattc agtgatagcg
    12421 atcgacgatc gacgcgcact tggcgtcgcc accggcaact tcgtggacac cgtagatcag
    12481 ctttacctga tcgacttggt gaccggtgac caggagctcg ttctcgagtc cggcgactcg
    12541 ttcgtcatcg gtctctcggc ttacgatcca gacagccgga tcctctacgt gcccgacgcg
    12601 ttcgaaaacg cggtaatcga gctcggagcc gatgacgacg gcttcgtgga gctgggttcg
    12661 actgagatcg caccgggcct cggtcttcct ccgagcaagg tttatctgct cgactgattt
    12721 cgaagatgcc cggagcaccg acgaggatat gcttggcggc gctccggttt agtcgccggc
    12781 cgggcttacc ttgccccgtg ccttcttgcg atctccgcgt cggcgctttt cctcgagacg
    12841 tcgacgcttg gcggctcgac tcggcttcgt cgcgactcga cgcgtcggcg gaatcaaggc
    12901 ctcgacgatc aatccgcgca agcggtcgcg tgctcgagtg agattctgac gttgcgaccg
    12961 ttcggcttgc gcgctcaggg acagcacgcc cgtggcatcg attcggttgg ctgcaagttt
    13021 tcgcaggcgc gccttctgcg gggcggttag cgcgttggtc gctcgcaggt cgaagcgaag
    13081 cgtgaccttc gtcgcgacct tgttcacgtt ctggccgcct ggccccgatg cgcgacttgc
    13141 ggtccaggaa aggtcgtgcg ccggaacggt tacacgctcg ttgatgacga ggtctttgtc
    13201 ggtcgcggga gcttcggagg tcatcgcgat tccaatgtgc gaacgaggcg ctagtcctcg
    13261 acccgggcaa gcacgtctcg aacccggtct ttccaaggca tcatcggacc tgcgacctcc
    13321 tcggtcgcgc ggatgtcgag aacgctgtac gcagggcgtg gcgcagggcg cgggaactcg
    13381 tcactcgtgc atggctccac gcggcaatcc ggattgactt cgtgggcgat gaaggcggcg
    13441 agctcgaacc aggtgcactc tcccgagtcc gcgacgtgga aggttccgcg ctgccccagc
    13501 tcagcgagcg ccaagctcac ttcggcgagc ctccgactgt ccgtcggacg tcctcgctga
    13561 tcgtcgacga cgcggaggct atctcggctc atggcgagct tccggatagt caggacgaag
    13621 ttctttgccc agggggcgta gagccaactg ctcctcacca gcaagtgttc cacgccggac
    13681 tgctcgatga gctcttctcc gagcgccttg ctacgcccgt aggcgttcac cgggtcgcga
    13741 ggatgatcga tcgggtaggg ctccgacgcg tttccattga aaacgtagtc ggtgctgtag
    13801 tggagcagct tcgcgccgac ttcccggcag cgttctgcca gacgtccgac ggcgcgccca
    13861 ttgatttcgt tcgcgagatc ctcgtagtcc tcggcggcat cgaccagcgt gtacgccgcg
    13921 cagttcacga cccattgcgt cccgggcttc acgaaccggg caacgttggc gcgatcggtg
    13981 atgtcgagat cttcgatcgt cgttcccaca tggtggatgc cgcgcgccgt caagagctcg
    14041 gcccagcagc gtccgagcat gccgtctgca ccgatgagaa cgaagtcatt catagcgggg
    14101 aagctgctcg agcgggatgt cctcgagccg aaaattctgc tgatctttgc gggagagttt
    14161 cggagattgt atgggccagg cgatgccgat gtctggatcg tcccaggcga tgccaagctc
    14221 gctcggagct gagtaaaact ccgtgcactt gtacgagaac aaggcctgct cgctgagtac
    14281 gcagaaccca tgcgcgaagc caggcggaac gtaaaactgc cgcttgttct cggcggaaag
    14341 cgtgaggccc tcccagctac caaaggtagg tgaccccacg cgcacgtcga cggcgacgtc
    14401 gaatacctct ccagcgagca cgaagcagag cttgccctgt gccttcggat gctgaaggtg
    14461 gaggccgcgc aaaatgcccc gcgcagagag agacaggttg tcctgaacga actcgccagg
    14521 aagcccggct tccacgtagc ggtcgcgcga gtaggactcc atgaagaagc ctcgctcgtc
    14581 cccgtggacg tcaggctcga cgacgagcag ccccggaatt ctcgcagccg cgactttcat
    14641 cagatgtcga ggcctctcgc aagggcgtgc aggtactctc cgtactccgt cttcgccagc
    14701 tgcttggcga ggcgctcgag ttgctcttcg tcgatccagc ccatgcgaaa tgagatctct
    14761 tcgggacaag acgcgcgcaa tccttggcgc tcctggatgg tttgtacgaa gttcgcggcc
    14821 tgaagcaagg ccactggagt tccggtgtcg agccatgcca caccacggcc gaacttctgc
    14881 aaccgcagct ttccaacgct caggtaccgc cggttgagat ccgtgatctc gagctcgcct
    14941 cgcgcggagg gcttgagcgc tttcgcgtgg gcaaccacgt cacggtcgta aaagtaaagc
    15001 cctgcgaccg cgtagttcga tttgggttcc ttcggctttt cctcgagacc gaccacgacg
    15061 ccgtcttcgt cgaactccgc caccccgtag gcggttggat tgttcacgta gtagccaaaa
    15121 accgtcgcac cttcggtctg acgggcgctt tccgccaaca agccctgaag cccgtgcccg
    15181 tagaagatgt tgtctccgag caccagggcg caggcctccc cgtcgatgaa gcgttcaccg
    15241 atcaagaaag cctgggcaag tccatccggg ctcggctgca ctgcgtactc gatgcgaagc
    15301 ccccaactct ctccgtcccc gagcaggccc tcgaaaagcg cgcgctcgtg tggggtcgta
    15361 atgaccagaa tctcacggat ccccgcgagc atcagcgtcg acagcggata gtagatcatc
    15421 ggcttgtcgt agatgggcat gagctgcttg ctcagtccct tggtcaacgg gtgcagtcgc
    15481 gtcccggtcc cgcccgccaa aatgatgcct ttcttgatca ttcctgctct ctattccgtg
    15541 cggtcgccgt agttcagatc catccacttg cggtagtcgc ctgagcggac gtctgcgacc
    15601 cagtcgtgat tggccaagta ccattgaacc gtcttgcgga tgccagtgtc aaaggtttcc
    15661 ttgggttccc acccgcattc ctcgcggata cggctcgcat cgatcgcgta acgcagatcg
    15721 tggcctggcc ggtccgtgac gaactgcttg agcctcagga gctcctcggg atcagagccg
    15781 gtttcctccg ccatgatgcg gcaaaggata tcgacgactt cgatgttcgt catctcgttg
    15841 ttgccgccga cgttgtaagt gcgacccacg cgtccttttt gaatcaccgt ccaaatcgca
    15901 tcgcagtgat cggacacata gagccagtcg cgcacgttga gccccttgcc atagattggc
    15961 agaggcttcc gctcgagcgc attcagaatc ataagcggga tgagcttctc gggaaactgc
    16021 atcggcccat agttgttgga gcaattggtg atcttgatcg gcagaccgta tgtccggtgg
    16081 taggcacgca cgatgtggtc acttgccgct ttcgaagccg aatacgggct cgaagggtcg
    16141 tacgaagtcg attcttcgaa gagtcccgta tccccgagcg agccgtacac ctcgtccgtg
    16201 ctcacgtggt gaaagaggtg gtggtcatcg cgcgaccagc gtttccgaca cgcttcgagg
    16261 aggttgaacg ttccctcgat gttcgtgcga atgaactcac gcggtccgag aatgctccgg
    16321 tcgacgtggc tctcggctgc gaagtgcacg acgagatcgg gatcaacttc atcgaagacc
    16381 gcgtcgacgg cgttcatgtc ggtgatgtcc acccgccgca gcccgtagtt cggcaaagag
    16441 tcgatcgacc tcaagctgcc gaggttcgcc gcgtaagtga gcttatcgag attgatgaat
    16501 tgatggtccg gaagctccgg caccaatcga ttcaggaaat ttgcgccgat gaagccggcg
    16561 ccgccagtga ctatgatctt catcgactgc tttcggcctc gacgattagc ataaccggaa
    16621 gatttgaccg gcgcacccgg tgttcgagac tatgggagca ggggtcagcc gcccagatgg
    16681 aacgtcagcg cgaaggtgcc cgcgattgcc gtccccgccg tgcccgccgt gaactggcct
    16741 cccgtcaccg tttcggttcc tcgaaagacg agaccgatga ccgagtagtc gaagtccgcc
    16801 gcgatggaga cccatctcac gatgggaata cgaagacctg caccgatgct cgcaccgccg
    16861 ccgttgctgc ggacgttgtt caccaggttt tcgaatcccg ggatgacgac cccgcccgtg
    16921 gtggtgcagt agttgaaacc aagtcggatc gacgggtgca cgtacggcac cgcacggatg
    16981 aggaatccga ggtccaagcc gaccgtcgtc aggtcgaaag gctcgtagtc ggcgcgcttg
    17041 tatcgcgcac cgatcgtcgt tgacccggcg cgaaagcgga gcgccgcacc aaactcggga
    17101 cccctcactg agacgtctgg caccagctgt tgtacccctg ctggaacgat gtcgagtgtt
    17161 cggaatcgag ttgcgtcgaa gcgcgaagga cctgccgtcg cctcgagcca aacgatgccg
    17221 ccgaccttct tgttgtatgc cttcttcttt tcggggacgt cttcacggac ctcttttccg
    17281 gagccagtca gcgagactcc gttctgttcg accgagaaag tccctggtag gtcctgcgcg
    17341 cttgcgccgg ctgccaccgt caaaattgtt ccgagcacga aagcaaagcg cttcatcggt
    17401 tccccctaac cgctgattcg acatgctcct tcaggagcca catcccccgt cgatcag
//
LOCUS       DASRZF010000135         2762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3183898, whole genome shotgun sequence.
ACCESSION   DASRZF010000135 DASRZF010000000
VERSION     DASRZF010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3183898"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /gene="rlmN"
                     /locus_tag="VFG22_06215"
     CDS             <1..750
                     /gene="rlmN"
                     /locus_tag="VFG22_06215"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine(37)-C2)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine(2503)-C2-)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HET6415870.1"
                     /translation="QCISSQVGCAMGCVFCASGVAGLKRQMSDAEIVSQVLLGRRELG
                     PEQRIRNVVFMGMGEPLHNYDAVARALVLLTHPEGIDLSKRRVTVSTSGLVKQIDQLG
                     KDFDGQVQLAISLHAVRDDERSKIMPVNRKHGLKQLTEALRRYPMSKRQRITIEYTLI
                     EGVNDSLADANGMVELLRGIPVKVNLIPMNPISASELRAPDSGHVTRFQEQLTRRGLS
                     VFVRKTRGDDVAAACGQLAFHGESRRAAGRE"
     gene            complement(771..1889)
                     /locus_tag="VFG22_06220"
     CDS             complement(771..1889)
                     /locus_tag="VFG22_06220"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:NF013836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HET6415871.1"
                     /translation="MASQDIDAQTAQVLHRFGFEDIPFESLKARLVRGELGAAQNRIK
                     GLVEPPDEGDLRALPPLGGDVRRALAERGEQAMRSGEVAAVILAGGMATRFGGVVKAA
                     VEVLDGQSFLELKLKDIAAVAARAGATIPVYLMTSFATDDAIRRMAEELSTERCPIKT
                     FPQFISLRVTPDGELFREGSGELSAHAPGHGDLTFALRRSGILADFRKRGGRVLMMSN
                     VDNLTATLDPAVIGAHLESGAALTAEMAPKEPGDKGGAPARVDGKAQIVEAFRFPESF
                     DQDRIPVFNTNTFVLDAAAIDADFPLTWFAVNKTVDGRPAVQFERLVGELTAFLESSF
                     LRVERHGPDARFQPIKTPDDMDKERPDIVKALKARGCI"
     gene            2003..>2762
                     /locus_tag="VFG22_06225"
     CDS             2003..>2762
                     /locus_tag="VFG22_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415872.1"
                     /translation="MTCPIRFWVFFALSVAAAMGGSARADYDGPDGYGSSSPSEFDVS
                     HDGYTREGQTPAAFDSSTYVVIEEEDGETEQVDGETVIVVQEPEPIAATAQAPPAPRT
                     VVVENTINLCPGGIWVDGYWGYGNGDYIWVDGHCVVERVNYVFVQPRWDFYSSVWWFV
                     PGYYRPCGVYVGFGYYRPWFWFPPYFHSFYPARRPVPVYRSVARRPTTTRVTPASRPP
                     SRRVGVTPGRTSTVRRAPITRTSTVTRAPSMRTST"
BASE COUNT          499 a          865 c          874 g          524 t
ORIGIN      
        1 cagtgcatat cgagtcaggt cggctgcgcc atgggatgcg tattctgtgc ttccggggta
       61 gccggcctca agcgccaaat gagcgacgcc gaaatcgtct ctcaggtgct cctcggccga
      121 cgtgagctcg gcccagagca gaggatccga aacgtcgtct tcatgggcat gggcgaaccg
      181 ctccacaact acgatgcagt agcgcgtgcg ctcgtgctcc tcactcatcc ggagggcatc
      241 gacctttcca agcggcgcgt caccgtatcg accagcggcc tggtcaagca gatcgatcaa
      301 ctcggcaagg atttcgacgg tcaggttcag ttggcgattt cgttgcatgc tgttcgcgac
      361 gacgagcgaa gcaaaatcat gccggtgaac cgcaagcatg gtctcaagca gctgaccgag
      421 gcgctacgcc gttacccgat gtccaagcgt caacgcatca ccatcgagta cacactgatc
      481 gagggcgtga acgattccct cgcggacgcg aacggcatgg ttgagcttct tcgtgggatt
      541 ccggtgaagg tcaacttgat cccgatgaat cccatttccg cctccgagct gcgcgcgccg
      601 gactcgggcc acgtgacgcg gtttcaggag cagctgacca gacgcggcct ttccgttttc
      661 gtccgcaaga cgcgcggaga tgatgtggcg gcagcctgcg ggcagctagc gtttcacggg
      721 gagtcgcgca gggccgcggg acgcgaatag cgccctacgc ggccccgggg ctagatacag
      781 cctcgcgcct tgagggcttt gacgatgtct ggtcgttcct tgtccatgtc gtcgggcgtc
      841 ttgatgggct ggaagcgtgc atctggtccg tgccgctcga cgcgaagaaa cgaagattcg
      901 aggaacgctg tgagctcgcc aacgagccgc tcgaattgca ccgctggcct gccatctacc
      961 gtcttgttca ccgcgaacca ggtgagcggg aagtcggcgt cgatggccgc ggcgtcgagc
     1021 acgaaggtat tggtattgaa cactggaatt cgatcctgat cgaagctctc cggaaagcga
     1081 aaggcttcga cgatctgcgc cttcccgtcg actcgcgcgg gcgcgccgcc cttgtccccg
     1141 ggttccttcg gcgccatctc tgcggtcagc gcggcgcccg attcgaggtg ggcgccgatc
     1201 acggcgggat ccagggttgc cgtgagattg tcgacgttcg acatcatcag cacacggcca
     1261 ccgcgcttgc gaaaatctgc gaggattccc gagcgccgca atgcgaacgt gaggtctccg
     1321 tggccaggcg cgtgggcaga gagctcgccc gagccttcac gaaaaagctc gccgtctgga
     1381 gtcactcgaa gggagatgaa ctgcgggaac gtcttgatcg ggcagcgctc cgtgctgagc
     1441 tcttcggcca tacggcggat cgcgtcgtcc gttgcgaagc tcgtcatcaa gtaaactggg
     1501 atcgtagcgc ccgcacgcgc tgctaccgcg gctatgtctt tgagcttcag ctctagaaaa
     1561 gactgcccgt cgagaacctc gaccgcagcc ttcacgactc cgccaaaccg tgtcgccatg
     1621 ccaccggcca ggatcactgc tgcgacctca cccgagcgca tggcttgctc gccccgttcg
     1681 gccagcgcac gccgcacgtc gcctccaagc gggggcaggg cgcggagatc gccttcgtcc
     1741 gggggctcga ccaggccctt gattcggttc tgcgccgcgc caagctcgcc gcgaaccagg
     1801 cgtgctttga gcgattcgaa gggaatgtcc tcgaatccga agcgatgaag aacttgggcg
     1861 gtctgcgcgt caatgtcttg tgaggccatc agaattgcca aaacccaagc actttccatg
     1921 cgctctgtcg agcgggccgc gcttgctcga gggcgagggg gtgacggcag gcccaattgg
     1981 acgcacagat agagcaggga ctatgacttg ccccattcgt ttttgggttt tcttcgccct
     2041 ctcagtggct gcagcgatgg ggggctccgc gcgcgccgat tacgacggcc ctgacgggta
     2101 cggctccagc agcccttccg agttcgacgt cagccacgat ggctacacac gagaggggca
     2161 gacccctgcg gccttcgact cgagcaccta tgtggtgatc gaggaagaag acggcgagac
     2221 cgagcaggtc gacggggaga cggtcatcgt cgtccaggag cccgagccga tcgccgcgac
     2281 tgcacaggcg cccccggctc cacgcactgt cgtcgtcgaa aataccatca acctctgccc
     2341 tggaggcatt tgggtggacg gatattgggg atacggcaac ggtgactaca tctgggtgga
     2401 cggacactgc gtcgtcgagc gtgtgaacta cgttttcgtc caacctcgct gggacttcta
     2461 ctcgagcgtt tggtggttcg taccgggcta ctacaggccc tgtggcgtct acgtagggtt
     2521 cggatactac cgtccatggt tctggtttcc gccatacttc cattcgttct acccagcgcg
     2581 tcgtccggtt ccagtgtacc ggagcgttgc acgccggccg acgacgactc gcgtaacgcc
     2641 agcgtctcgc cctccgagcc ggcgcgttgg tgtgactccg ggccgtacct cgacggtgag
     2701 gcgcgcaccg atcacgcgaa cgtcgaccgt gacgcgcgca ccgagcatgc gaacgtcgac
     2761 cg
//
LOCUS       DASRZF010000136         3827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2497483, whole genome shotgun sequence.
ACCESSION   DASRZF010000136 DASRZF010000000
VERSION     DASRZF010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2497483"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="VFG22_06230"
     CDS             complement(<1..664)
                     /locus_tag="VFG22_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415873.1"
                     /translation="MKSRLVLAVALCLLGFTCDETGAGPEAASKPGAGQPSQESADKS
                     PTEGPGAATPAPAVAPAPNGTAPYEDLLALLHLADIYDPAGLFIEFGTSARLKYTLGN
                     WNSGWQAESREGGATVSTFAKSARLYLPAATVQPMRLRIRARVYATGALIVYVNGETA
                     GEARVTQGGGFQDIVIPISGAHIREGENAIMLRATQTAQVRGEARSLALDWLRFEPAS
                     STD"
     gene            756..2681
                     /gene="speA"
                     /locus_tag="VFG22_06235"
     CDS             756..2681
                     /gene="speA"
                     /locus_tag="VFG22_06235"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF003763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HET6415874.1"
                     /translation="MGLMDGNGEPREWSVKKSAKLYQIKGWGEPYFRINEDGHVEVRP
                     DPTEEQAIDLYELTKQLAERGLDLPLLIRFPNILQDRIRLLNESFQSAIAQYGYEGTY
                     RGVFPVKVNQQRHLVEEIVESGRPWRFGLEAGSKPELLITLAAMEDEDGLIICNGYKD
                     LAYMETALVAQRFDNTVVVVLERVEELELAFRASEKLGIRPCLGVRAKLSSKGMGRWA
                     DSAGDRAKFGLSMSEIVHVVDELEKRDMLDCLQLLHFHLGSQVSSIIPVKNAMREAGQ
                     IYVELVKMGCEMRFLDVGGGLAVDYDGSKTDFSGSMNYDLQEYAYDVVAGIQDACEKA
                     DVKVPTIVSESGRSIAAYQSVLVFDALGVDHVDFEDPEAPSPDDHRVLDEFYETWKGI
                     QPKNVQESWHDAQQALEEARSLFKFGYLGMRELARAEHLFWSCCEKIRQNMRRLKYVP
                     EELNAVEDLMGAIYYCNFSLFQSAPDIWAMDHLFPFMPIHRLDEEPTVRARLADLTCD
                     SDGIIDHFIDVEEVQSSLDLHSVKPGEPYILGMFLGGAYQEILGDLHNLFGDTNAVHV
                     RLEDYGYSVANVIKGDSIDEVLRYLQYDPEEMVERVRRQAERALNQGRMTLPQLRTFM
                     QHYEESLRGYTYLKGDA"
     gene            2740..3480
                     /locus_tag="VFG22_06240"
     CDS             2740..3480
                     /locus_tag="VFG22_06240"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HET6415875.1"
                     /translation="MDVAEFQEALLQQMERKTHWAWPAFTAGMVAKDKLHIHLEQEWE
                     VYVRDFPIMVGRAYVQCPVAAVRRELAENLYEEETGGLAAGKPHPDLFMMYPLGLGMD
                     VERFSHVELLPAARAYREFLDEVTTGRGWSVAACIATLFIEGTAYERHELDPAAPARP
                     QPPLEDHPLAKHYGLPVECLALTKAHRSVEGEHRKAAWRVMLNHLPANDRPAVVAAMS
                     QAVDAWRVYRDGVAEACGVKREALSLTA"
     gene            complement(3469..>3827)
                     /locus_tag="VFG22_06245"
     CDS             complement(3469..>3827)
                     /locus_tag="VFG22_06245"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HET6415876.1"
                     /translation="TGDLATRTAEGAIRILGRRSTDLIKTGGYRVGAGEIEASLLEHP
                     SVREVAVVGVPDDDLGQRITAFVVLRPGEPEDPDSLTRFVAENLSPHKRPRDVRFMQE
                     LPRNAMGKVQKKLLGG"
BASE COUNT          751 a         1148 c         1214 g          714 t
ORIGIN      
        1 cgtcagtgct ggaagccggc tcgaaacgga gccagtcgag cgcgagtgac cgagcttctc
       61 cccggacctg ggccgtctga gtcgcgcgca gcatgatcgc gttctcgcct tcccgaatgt
      121 gagctccgga aatcgggatg acgatgtctt gaaagccgcc gccttgcgtc acgcgcgcct
      181 cgcccgccgt ctcgccgttg acgtacacga tcaaggctcc agtggcatag accctcgcgc
      241 ggatccgcaa ccgcatcggc tgaacggtcg cagccggaag atagagacga gcggacttgg
      301 cgaaggtcga cacggtcgcg ccgccttcac ggctctcggc ctgccagccg ctgttccagt
      361 ttccgagggt gtacttgagt cgggcgctgg tgccgaactc gatgaagagg cccgctgggt
      421 cgtagatgtc ggccaagtgc agcaaggcga gaaggtcctc gtagggggcc gtcccgtttg
      481 gagcaggggc gaccgccggt gccggcgtgg ccgcgccagg tccctccgtg ggcgatttgt
      541 cggcgctctc ttgcgatggc tgcccggcgc caggcttgga tgcggcctcg ggccctgccc
      601 cggtttcgtc acatgtgaat cccagcaggc atagagcgac ggcaagcacc aggcggcttt
      661 tcatagaagt tccccttcgc gcgttcggct accacggtcg ggccagcggg gcaacggcgc
      721 cgcggcgacc acgagccctc agtttatgct acttgatggg cctcatggac gggaacggcg
      781 aaccgcgcga atggagcgtg aagaagagcg cgaagctcta ccagatcaaa ggatggggag
      841 aaccgtactt ccgcatcaac gaagacggtc acgtggaggt ccggcccgac cctaccgagg
      901 aacaggcgat cgacctctac gagctcacca agcagttggc tgagcgcggg ctcgatcttc
      961 cactgctgat tcgctttccg aacattctcc aggaccgaat tcggctgctg aacgaatcgt
     1021 tccaaagcgc gatcgctcag tacgggtacg agggcacgta tcgcggtgtc tttcccgtca
     1081 aggtcaatca gcagcggcat ctcgtcgagg agatcgtcga atcgggaagg ccatggagat
     1141 tcggtctcga agcaggctcg aagcccgagc tgctgatcac gctcgcggcg atggaggatg
     1201 aagacggcct catcatttgc aacggataca aagacctcgc ctacatggag acggcgctcg
     1261 tggcgcagcg attcgacaac accgtcgtcg tagtgctcga acgcgtcgag gagctcgagc
     1321 tcgcttttcg cgcctccgag aaactcggca tcaggccgtg cctcggcgtc cgcgccaaac
     1381 tgagtagcaa aggaatgggt cgctgggccg attccgcggg cgaccgcgcg aagtttggat
     1441 tgtccatgtc ggaaatcgtc catgtcgtcg atgagctcga gaagcgggac atgctcgatt
     1501 gtctgcagct cctccacttc cacctaggaa gccaggtttc cagcatcatc cccgtgaaaa
     1561 acgccatgcg tgaggcgggg cagatctacg tggagctggt gaaaatgggc tgcgagatgc
     1621 gcttcctcga cgtcggcggt ggtctggcgg tcgactacga cggctcgaag accgacttca
     1681 gcggctcgat gaactacgac ctccaagagt acgcgtacga cgtagtcgcg gggatccagg
     1741 atgcctgtga gaaggcggac gtgaaagtgc cgacgatcgt gagcgagagt ggccgctcga
     1801 ttgcagctta tcagtcggtg ctggtcttcg atgcgcttgg cgtggaccac gtggacttcg
     1861 aagacccgga agccccttca cccgatgacc atcgtgtcct cgacgaattt tacgagacct
     1921 ggaagggcat acagccgaag aacgtgcagg aatcctggca cgacgcacag caggccctcg
     1981 aggaggcacg aagcctcttc aagttcggat atctcgggat gcgcgagctg gcgcgcgcgg
     2041 agcacttgtt ctggagctgc tgcgaaaaaa tccggcagaa catgcggcgt ctcaagtacg
     2101 tccccgaaga gctcaatgcg gtcgaagacc tgatgggtgc gatctactac tgcaacttct
     2161 cccttttcca gtcggcgccg gacatctggg ccatggacca tctctttccg ttcatgccga
     2221 ttcaccgcct cgatgaagag cccacggtgc gcgctcgctt ggcggacctc acctgcgact
     2281 cggacgggat catcgaccac ttcatcgatg tcgaagaagt tcagagctca ctcgacttgc
     2341 attcagtaaa acccggcgag ccctacattc tcggaatgtt cctaggcggt gcataccaag
     2401 agattctcgg cgacctgcac aatctgttcg gcgatacaaa cgcggttcac gttcgcttgg
     2461 aagactacgg atacagtgtg gcgaacgtca tcaaggggga ctcgatcgac gaagtcctgc
     2521 gttaccttca gtacgacccc gaagagatgg tcgaacgagt tcggcggcaa gcggagcgcg
     2581 ctctcaacca gggccgcatg acattgcccc agctgcgaac cttcatgcag cactacgaag
     2641 aaagcctgcg cggctacacc tatctcaagg gcgacgcctg aaattcactg attttcgggt
     2701 gttttcgaca gcgggccagc caagctctac cttgaaatgg tggacgtagc ggagtttcag
     2761 gaagcgctcc tccaacagat ggagcgaaag acccattggg cgtggccggc gttcaccgct
     2821 ggcatggtcg cgaaggacaa attgcacatc catctcgagc aggagtggga agtgtatgtg
     2881 agggacttcc ccatcatggt ggggcgcgcg tacgtgcagt gcccggtcgc cgcggtgcga
     2941 cgcgagctcg ccgagaacct ctacgaggag gagaccgggg gactggcggc tggaaaaccg
     3001 caccccgatc tgttcatgat gtatccactc ggtctcggca tggacgtcga gcgcttttca
     3061 cacgtcgagc tcttgccggc ggcgcgcgcg tatcgtgagt ttctagacga ggtgacgaca
     3121 ggacgcggtt ggtcggtcgc agcatgcatc gccacgctct tcatcgaagg gactgcctac
     3181 gagcggcatg agctcgaccc tgccgcgccg gcccgccctc aaccgcctct cgaggaccac
     3241 cccctcgcga agcactacgg tttgccggtt gaatgtctgg cgctcaccaa ggcgcatcgg
     3301 tccgtcgaag gcgagcatcg gaaggccgcg tggcgcgtga tgctgaatca cttgcccgca
     3361 aacgatcgac ctgccgtggt cgcggcgatg agccaagcgg tcgatgcctg gcgcgtttac
     3421 cgcgacgggg tagcggaagc ctgcggcgtg aagcgagagg cgctgtcgct aaccgcctag
     3481 aagctttttt tgcactttgc ccatggcgtt tctgggcagc tcttgcatga aacgaacgtc
     3541 tcggggccgt ttgtgcgggc ttaggttttc ggcgacgaag cgcgtgagag agtctggatc
     3601 ctccggctcg ccggggcgaa ggacgacaaa ggcggtgatg cgctgcccaa gatcatcatc
     3661 cggaactccc acgaccgcga cctctcggac cgaggggtgc tcgagtaagg aggcttcgat
     3721 ctctccagct ccgacgcggt acccgcccgt cttgatcaga tcggtgcttc ggcggcccag
     3781 gatgcgaatc gctccctccg ccgtgcgtgt tgcaaggtca cccgtga
//
LOCUS       DASRZF010000137         6572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3529328, whole genome shotgun sequence.
ACCESSION   DASRZF010000137 DASRZF010000000
VERSION     DASRZF010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 6572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6572
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3529328"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..736)
                     /locus_tag="VFG22_06250"
     CDS             complement(35..736)
                     /locus_tag="VFG22_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415877.1"
                     /translation="MYAQLKTFGLLLFAVAIAACGDDGNGTAGGGGAQTSTVTGTVYF
                     YEEGYPAVEGATVEVYETSLSTTTDSQGNFTLEGVPHGNRFFITKAAGSWGLVDYWYV
                     PDETPNGGAAELLVVRDAEVASWATAIGRPINDNHAMVDVIFQEGAQGGETATIDADS
                     DPPFTFSGWDAVDQQTLIADVDGFGELVFPNINPPSGTVDAEVMPARCFIDESDGLRY
                     PILPKSVTIIYALCD"
     gene            1180..1995
                     /locus_tag="VFG22_06255"
     CDS             1180..1995
                     /locus_tag="VFG22_06255"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HET6415878.1"
                     /translation="MSSNFKNYENPSIFHRWAAAQWRRPKDPTIYGATEVDMEPALAF
                     VEKVREEWGVNVTVTHLVTKALAIAIARHPRTNAKVRFWGKLEQRKTVDITVLIAGAG
                     GRDLSAHRLIAADKLPLRQLATDVIEAGELIRADQDPKFQQTKQLMDKLPWWMMRPFL
                     TLASLSMNELHLDLSKLGLPADLFGVGMVTSLGMHGVDEAYAPLTPIGRIMVDVLLTR
                     VRPRPWVGDDGELGVRRTLKLCATFDHRVVDGIQAARICQELHSLLGDPDSLR"
     gene            2009..3280
                     /locus_tag="VFG22_06260"
     CDS             2009..3280
                     /locus_tag="VFG22_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415879.1"
                     /translation="MSAFDFFITLDGNCLNGFEGLCGIARLRCDPDADRWEKEVHFFE
                     GLAGGHATQVNPTGTLAFLGNLSQTLLFYDPRTLREVRRLSTLRFCAPEVLYSSQTHV
                     VWLSEKSFITVLGDSFWRFDMDDLEHPVRLGAHLVKLPHALKLSPSRRYIGYGSMDHD
                     IKGYARELGVFDLTTNEARVVQLPATCWHIAAHPTKDFFYGPSQQVAPQDGEFGEYTL
                     AHFKNYVFEVDAETATVTRHASIAKELPAAFTSDVVVTADDVFYNACAGGTLVRVDLE
                     TFKHVQFIDERPSFLRSLPHLRSGWSNLVESFSRVDVPGNSHLLLKALRATRFSLLDG
                     SLGLQVSPDGRFILSAHRGLNEVIVYRHPEMKVHKRIRFPSIRGFFPEHIGLLDDPRL
                     GFHHSTISTATVGASTIVRSTELPGGTGHQR"
     gene            complement(3277..3990)
                     /locus_tag="VFG22_06265"
     CDS             complement(3277..3990)
                     /locus_tag="VFG22_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415880.1"
                     /translation="MWKLSCLALAVLTFAPLARADDADDLPPGSQPEYGKMPMTSEGR
                     NPRLERRLSLQTRHRQDRTYRGPAGILGFQGGVPIFVGSDVDRDVVKAGGSILGFGGV
                     DFGFGTVELEVGWMGNPTDPPGLDGVTAQRVVLGVGGRVQVPNKSVVVPFVGFGFAAQ
                     WWKYDLPIGGCTALFCSTGGSFRFAPGFNVRAGLSFLLGGVAAIEIGARYNISFETNG
                     IATRNLQFVEPTLGFRFWL"
     gene            4112..4732
                     /locus_tag="VFG22_06270"
     CDS             4112..4732
                     /locus_tag="VFG22_06270"
                     /inference="COORDINATES: protein motif:HMM:NF024436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HET6415881.1"
                     /translation="MIKHSSSPQHLLQPLLVLASLVVLLAGCAGPRAVRGQDTPGLDQ
                     AAMSTSLDRKDLETALKINMEALRTSKLMARWQAEQDPTVAVLPIRNETSEHIDSALN
                     ALMTEIESQLINFAPVRVISLEDQPVLIEEIRQQQQNSAYDQSRVAAWGKQIGVRYIV
                     SGRVYATDERTQSARRVQYYLFLRTVDVETGEILFQNQAPITKAII"
     gene            4736..6139
                     /locus_tag="VFG22_06275"
     CDS             4736..6139
                     /locus_tag="VFG22_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415882.1"
                     /translation="MTEESKRQKVGRPHHLAALVFAATAVLATAGCKTFSDYTRQTRS
                     ALDVSDLDGALRASNRALDVKRADNVPEELKKNDALLLLDRAMVLQARQQYDWSSRDF
                     QIADKAIEILDFSRSTSDEIVRYMFSDSAGPYKARPYEKLMLNTMNMINYLAPGNLEG
                     ARVEARRFSVMRDYLLGAEKVDHDAVKQAGVPGSYLAGYVFEKSGAPDAALRYYDEVL
                     QATDAPSLTHPIQRLTQLGGYRTPRLESAAQGAGQGAPSSNESSEMLIVISYGRVPAL
                     LAQRVPIGFALTIAAAFLSPAAMQQARRLAAQGLVTWINYPDLAPSPKEYPAPLVTID
                     GQTQTVHVIADVDAVARRAWNESKGRVMAAAVTRMLTRAAVGAGAGVAVGKASGKSGI
                     GVLASMITQASMVAADKPDTRSWATLPARIAVVRAELPPGEHAIEVTVLGRRRRLDTT
                     IGPSEWVLVDVSELSTR"
     gene            6169..>6572
                     /locus_tag="VFG22_06280"
     CDS             6169..>6572
                     /locus_tag="VFG22_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415883.1"
                     /translation="MTPSPGQASLMWIWLPILAVPIVGPAIVGWKIWDEVRRTISEDP
                     LVWEKQIHAFEKKERRRPTAKGAVVFAGSSTIRFWSTLARDMAPLPAVQRGFGGAKVN
                     DVIHYMDRLITSHDPAMVVLYIGANDMLGFGG"
BASE COUNT         1250 a         2199 c         1939 g         1184 t
ORIGIN      
        1 tacgcgtgcg atcgatctgc gctgtgcctt tcgcctagtc gcaaagcgcg tagatgatgg
       61 tgacggattt cggtaaaatt ggataccgaa gtccatcaga ttcatcgatg aagcagcgag
      121 ccggcatcac ttcagcatcg accgtgccgg atggtggatt gatgtttgga aagaccagct
      181 cgccgaatcc atccacgtca gcgatcaggg tttgttggtc gaccgcgtcc cagccggaga
      241 acgtaaacgg aggatcgcta tcggcatcga tggtcgcagt ctctccacct tgtgcgcctt
      301 cttggaagat gacatccacc atcgcatggt tgtcattgat cggacggccg atcgccgtgg
      361 cccaagatgc cacctcggca tccctcacga cgaggagctc tgcagcgcct ccattgggag
      421 tctcatcggg cacataccag tagtcgacga ggccccagct tccggccgct ttcgtgatga
      481 agaagcgatt gccatgtggc actccttcga gcgtgaagtt tccttgtgaa tcagtcgtag
      541 tggagagact ggtctcatag acctcaacag tggccccctc gaccgccgga tatccttctt
      601 cgtagaagta cacggtgccg gtcaccgtcg acgtttgcgc gccgccaccg cctgcagtcc
      661 cgtttccgtc atcgccgcac gctgcgattg cgacggcgaa aagtagcagg ccgaatgtct
      721 tcagttgagc gtacataagc acctccccgt tcagagaaca caggctctcc gggtgcacga
      781 cacgtgccaa ccggtgcgat accacggttt ctcactgttt ccgggtccgg ccaagcgcat
      841 ggcttgcgct gagcacgcct tttgcgccag gattgcgcgc tgatcaggcg actggcggga
      901 accacgggaa gccagattct ggatttgaaa cccgctgcgc ggatgggccc gcgcttacaa
      961 acccaaggct gctcgctccg gcgcttcagg gctccgggcg agaccctccg ggctgacgat
     1021 caatacgtcc aagtactcct ctgcatcaga gccaggcccg cttccgtctt gggcattgtt
     1081 tcgtatatgg tggtctggtg cttgaaccaa ttgtctcggg caacacgtat gccgtgctga
     1141 agcacgtccc gttctcgaca gtatccgcgg agcgaagcca tgagcagcaa cttcaagaat
     1201 tacgagaacc cgtcgatctt tcatcgctgg gccgcggccc agtggcggcg tccgaaggac
     1261 cccaccatct acggcgcgac cgaggtcgac atggagccag cgctggcttt cgtggaaaag
     1321 gttcgcgaag agtggggtgt caacgtcacc gtcacgcact tggtcaccaa ggcactcgcc
     1381 atcgccatcg cccgccaccc gcggaccaac gccaaggtgc gcttctgggg caagctcgag
     1441 caacggaaga ccgtcgacat caccgtcttg attgcaggcg cgggtggccg cgacctctcg
     1501 gcccaccggc tcattgcggc cgacaagcta ccccttcggc agctcgccac cgacgtcatc
     1561 gaagcgggcg agctcatccg tgccgaccag gatccaaagt tccagcagac caagcagctc
     1621 atggacaagc tcccctggtg gatgatgcgc ccgttcctca cgctcgcgag cctctcgatg
     1681 aacgagctcc acctcgacct gtcgaagctc gggctcccgg cagatctctt cggcgttggg
     1741 atggtgacca gcctcggcat gcacggtgtc gacgaagcct acgcgcccct gacaccgatc
     1801 gggcgcatca tggtcgatgt ccttctcacg cgcgtgcgtc cgcgcccctg ggtgggagac
     1861 gatggcgagc tcggtgttcg gcgcacgctc aagctctgcg ccaccttcga ccaccgcgtc
     1921 gtcgacggca tccaagccgc ccggatctgc caggagcttc acagcctgct tggcgatccc
     1981 gattcgctcc gctgaagaga ggaacagaat gtccgccttt gacttcttca ttacgctcga
     2041 tggcaactgc ctcaacggtt tcgagggcct ctgcggcatc gcccgcctcc gctgcgaccc
     2101 ggacgccgat cgctgggaga aggaggtcca cttcttcgaa ggactggccg gcggccacgc
     2161 cacgcaggtg aacccgaccg gcacgctggc gttcctcggc aacctctccc agacactcct
     2221 tttctacgat ccgcgcaccc ttcgcgaggt gcgccgcctc tcgacgctgc gcttctgcgc
     2281 gcccgaggtc ctctactcca gccaaacaca cgtggtgtgg ctgtccgaga agagcttcat
     2341 caccgtgctc ggcgactcgt tctggcgctt cgacatggac gacctcgaac acccggtacg
     2401 cctcggcgcc cacctcgtga agctccctca cgcgctcaag ctctcgccct cacgacgcta
     2461 catcggctac ggctccatgg atcacgacat caagggctac gcgcgcgagc tcggcgtctt
     2521 cgatctcacg accaacgaag cccgcgtcgt gcagcttccg gccacctgct ggcacatcgc
     2581 ggcgcacccg accaaggact tcttctacgg cccgagtcag caggtcgcgc cgcaggatgg
     2641 cgagttcggc gagtacacgc tcgcccactt caagaactac gtcttcgagg tagacgccga
     2701 aacggccacc gtcacgcgcc acgcctccat cgccaaggag ctccccgcag cattcacctc
     2761 cgacgtggtg gtcaccgccg acgacgtctt ctacaacgcg tgcgccggcg gcacgctcgt
     2821 gcgggtcgat ctcgagacct tcaagcacgt gcagttcatc gacgagcgcc cgagcttcct
     2881 ccgctcgctc ccccatctgc gcagcggctg gtcaaacctc gtagaaagct tcagccgcgt
     2941 cgacgtgccc ggcaactcgc atctgctcct gaaggctctg cgtgctaccc gcttcagcct
     3001 cctcgacggg agcctcggcc tgcaagtgag ccctgacggg cgcttcattc tctctgccca
     3061 ccgcggcctc aacgaggtca tcgtctatcg ccatccggag atgaaagtgc acaagcggat
     3121 ccgcttcccc tcgatccgtg ggtttttccc cgagcacatc gggctcctcg acgatcctcg
     3181 tctcggcttt caccacagca caatctcgac cgcgaccgtc ggcgcatcaa cgatcgtgcg
     3241 gtcgaccgag ctcccaggag gcacaggtca tcaacgctag agccagaaac ggaagccgag
     3301 cgtcggctcg acgaactgca ggttccgcgt ggcaatgccg ttggtctcga acgagatgtt
     3361 gtagcgggcg ccaatttcga tcgccgccac gccgcccaac agaaacgaga ggcccgcccg
     3421 cacgttgaag ccgggcgcga agcggaagct ccctccggtc gagcaaaaca gtgcggtgca
     3481 gccgccgatc ggtaggtcgt acttccacca ctgtgccgca aagccgaatc cgacgaacgg
     3541 aaccaccacc gacttgttgg ggacctgcac gcggcctccc accccgagga cgacgcgctg
     3601 tgcggtcacc ccatcgagcc cgggcgggtc ggtggggttg cccatccatc cgacctcgag
     3661 ctcgacggtg ccaaagccga aatcgacccc gccgaaaccg aggatgcttc cgccggcttt
     3721 caccacatcg cgatcgacat cgctgccgac gaagatcgga acgccgccct ggaaccccaa
     3781 gatgcccgcc ggaccacgat acgtccggtc ctgccgatgc ctggtttgaa gcgacagtcg
     3841 gcgctcgagc ctcgggtttc tgccttcgga ggtcatcggc atcttgccgt actccggttg
     3901 cgagcctgga ggaaggtcgt ccgcatcatc ggctcttgcg agcggtgcaa acgtcaagac
     3961 ggccagggcg agacaggaga gcttccacat ggttaggaag cgtacggtag cacaggaatg
     4021 tggcggagcc cgtgcgcccg ggctctctgc tttgcgcttg ccgccggggt cgctacgcta
     4081 cgcaacccga actcattcgc acggcaagga tatgatcaaa cactcgtctt caccccagca
     4141 ccttctccaa ccccttctgg tcctcgcctc gctcgtcgtc ctcctcgccg gttgtgccgg
     4201 cccccgggcc gttcgcgggc aggacacccc gggcctcgat caggccgcca tgagcaccag
     4261 cctcgatcga aaagatctcg agacggcgct caagatcaac atggaggcgc tcaggacgtc
     4321 caagctgatg gcccgatggc aggcagagca ggacccgacc gtcgcggtcc tgcccatccg
     4381 aaacgaaacc agcgagcaca tcgacagcgc gctcaacgcg ctcatgaccg agatcgagtc
     4441 gcagctgatc aacttcgctc cagtccgggt gatcagcctc gaagaccagc cagtcttgat
     4501 cgaagagatt cgtcaacaac aacagaacag tgcgtatgat caatcgcggg ttgcggcttg
     4561 gggcaagcaa atcggtgtgc gctacatcgt ttcgggtcgg gtgtatgcaa ccgacgagcg
     4621 cacgcaaagc gctcgaaggg tgcagtacta tctgttccta cgcacggtcg acgtcgagac
     4681 gggcgaaatc ctcttccaga atcaggctcc catcaccaag gcgatcatct agttcgtgac
     4741 cgaggagtcg aagcgccaga aggtcgggcg gccgcaccat cttgcggcgc tcgtcttcgc
     4801 agcgaccgcg gtgctcgcca ccgccggttg taagacgttc tcggactaca ctcgccagac
     4861 tcgcagtgcg ctcgacgtga gcgacctcga cggcgctctg cgcgcaagca atcgggcgct
     4921 cgacgtcaag cgcgccgaca acgtaccgga agagctgaag aagaacgacg cgctattgct
     4981 tctcgatcga gcgatggtgc tgcaggcccg gcaacagtac gactggtcga gccgcgactt
     5041 ccagatcgct gacaaggcga tcgagatcct cgacttctcg cgctcgacct ccgacgagat
     5101 cgttcggtac atgtttagcg atagcgcagg tccctacaaa gcccgtccgt acgagaagct
     5161 catgctcaac acgatgaaca tgatcaacta cctcgctccg ggcaatctcg agggcgccag
     5221 agtcgaggcg cggcgttttt cggtcatgcg tgactacctg ctcggcgccg agaaggtcga
     5281 tcacgacgcc gtgaagcaag cgggcgtgcc cggaagctat ctcgcggggt acgtcttcga
     5341 gaagtcgggt gctcccgacg ctgcgcttcg atactacgac gaggtcctac aggcgactga
     5401 cgcgccgagc ttgacgcatc cgattcaaag gctcactcag ctcggtggct accgaacccc
     5461 ccgcctcgag tccgccgccc aaggtgccgg gcagggcgcg cccagttcga acgaatcgtc
     5521 cgaaatgttg atcgtgatca gctacgggcg tgttcctgct ctcctggccc agcgcgtccc
     5581 gatcggcttt gcgttgacca tcgcggcggc ttttctgtct ccggctgcga tgcagcaggc
     5641 gagacgactt gcggcgcaag ggttggtcac ttggatcaac tatccggatc tcgcgccgtc
     5701 acccaaagag taccccgcgc cgttggttac gatcgacggg cagacccaga cagtacacgt
     5761 gatcgccgat gtcgacgcgg tcgctcggcg ggcgtggaac gagagcaaag ggcgcgtcat
     5821 ggccgcggcg gtgacccgca tgctcacccg agctgcggtc ggagcgggcg cgggggtcgc
     5881 tgtcgggaag gcgagcggta agagcgggat cggcgtgctc gcttcgatga tcacacaggc
     5941 aagcatggta gcggccgaca agcccgacac ccgaagctgg gcgactcttc ctgcgcggat
     6001 tgcggtcgtc cgcgccgagc ttcccccagg cgaacacgcg atcgaagtga ccgttcttgg
     6061 gcgacgacgt cgtctcgaca cgacgatcgg accgtctgaa tgggtcttgg tggacgtcag
     6121 cgagctctcc acccggtagc cgctgcccca caacggagga ccctggccgt gaccccaagt
     6181 ccaggacagg cttcgctcat gtggatttgg cttcccatcc ttgctgtccc catcgtcgga
     6241 cccgccatcg taggctggaa gatctgggac gaggttcgca ggacgattag cgaggacccc
     6301 ctcgtttggg aaaaacagat ccacgcgttc gaaaagaagg aacgcaggcg acccactgcg
     6361 aagggagctg tcgtctttgc ggggagctcg accattcggt tctggagcac ccttgcgcga
     6421 gacatggctc cactccccgc cgtgcagcgg ggcttcggcg gcgcgaaagt caacgacgtg
     6481 atccactaca tggaccgcct catcacgtca cacgatccag ccatggtcgt cctctacatc
     6541 ggtgccaacg acatgctcgg cttcggtggc aa
//
LOCUS       DASRZF010000138         8142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1468906, whole genome shotgun sequence.
ACCESSION   DASRZF010000138 DASRZF010000000
VERSION     DASRZF010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 8142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8142
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1468906"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..1308)
                     /locus_tag="VFG22_06285"
     CDS             complement(16..1308)
                     /locus_tag="VFG22_06285"
                     /inference="COORDINATES: protein motif:HMM:NF016915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415884.1"
                     /translation="MKRVRGFVDLVFDLVDESTNLVQRTHDSVVERSKRRFAPIEPAK
                     STAELVTGVQTAISGGIFASIRVLNGIARFGVNAAADVAEAGLEQQTDAESLELATPV
                     SSSALGTATWYVDYLQASINGFWGDHLSRKNNRLDLGMTLRHHGRHLPATPEAFAAAF
                     PSPTPKLCVFVHSLAATEWLWSLSSEKHYGDPDVTFGTRLRDDLGFTPIYLRYNTGRH
                     ISENGRALAKLISEVVDAYPVPVEEIALVGHSMGGLVARSAAHYAREHDEPWIGKLRH
                     VACIGSPHLGAPLEKAVNVFTHVLRKLDAAGAEVPAALLDSRSSGVKDLRYGYTVDEE
                     WAGRNPDEVFADARENVPLVEGVGYYFLAATISHDPEHPLGELLGDLMVRLPSAIGEA
                     PEPARRIHFSGGTVFPGMNHIHIANHPDVYEALRGLFA"
     gene            complement(1373..2656)
                     /locus_tag="VFG22_06290"
     CDS             complement(1373..2656)
                     /locus_tag="VFG22_06290"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET6415885.1"
                     /translation="MTTSQNDFESLRVGENLRRALRDVGYTEPTPIQSQAIPHLLQGR
                     DLLGIAQTGTGKTAAFALPLLQRLDEERSPAKPRRPRALILAPTRELALQIHEELGRY
                     GRYLSLRHTYIFGGVSANPQIKALAQGIDVVVATPGRLLDLAGQRKIDLSEVSVLVLD
                     EADRLLDMGFVRDVKRIVAMTPKTRQSLLFSATMPKEVVSLAREILRDPIRVDVSPKE
                     VTVSEIDQRVVMVGTNDKQRMLEHLLRDDEVSRAIVFTRTKHGANRVAKKLERAGIGA
                     EAIHGNKSQNARQRALSNFKNGDAWVLVATDIAARGIDIDAVSHVFNFELPHEPESYV
                     HRIGRTGRAGATGAAWSLVDPSERGRLRAVERLIRSSPSEVSVDLPPRDPAEQIRDRE
                     DERPRRDARPPRNRQRSGERPGEGQRPRRRRRPAA"
     gene            complement(2670..2951)
                     /locus_tag="VFG22_06295"
     CDS             complement(2670..2951)
                     /locus_tag="VFG22_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET6415886.1"
                     /translation="MSKKLFVGGLSWNTTDSELQQAFEACGTVAEAKVITDRETGRSR
                     GFGFVTFEDEQSAVRACEELNGSTLDGRTIRVDQANERPRNDRRSDNRW"
     gene            3222..3965
                     /locus_tag="VFG22_06300"
     CDS             3222..3965
                     /locus_tag="VFG22_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415887.1"
                     /translation="MKNLSFHVLSMILTVSLVLGCQSKGPTPKEGNAPGEPSPRVGTT
                     ETTGAHPGGTADPHAGMAAAGDPHAGMAPTQNPHGGTEVTGLPDDNGMLDVGAVAFKV
                     PGAWSVRPPKSQMRLAQLEAQGKAGPAELIVYFFGPQGAGTSDANIDRWIGQFANTDG
                     SPVTNATRSSSKVSGYDVARVEVAGEYGGGMAPPGQAQEAVPGQRLLAAIVASEGGPY
                     YFKFLGPDATVQEHREAFDGLIASIVPSP"
     gene            4040..5107
                     /locus_tag="VFG22_06305"
     CDS             4040..5107
                     /locus_tag="VFG22_06305"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HET6415888.1"
                     /translation="MIGTREQSSQVRPTDLRRRSRFVQVLAFSHSSAVSPAWRRLMTT
                     STYVLTWAGLTALIPLWIPIAFLVGVVRRCSFVILRLLTFFWFYLTIELMGLIAAGWI
                     HLVTRGNDRRRQDLMFQLECWWGSSLFSWLRRLLSLSVSIEGEEQILPGPVLVFIRHA
                     SIIDTAVPVALLSTRTGLRLRYVFKRELLVDPCIDVAGHASPNYFIDRGGSPEQELAG
                     VRKLAENLGDEGVLLYPEGTRFTKRKQEIALARLAKAHPDLVPTATSFRHCLPPKPGG
                     VLTLLDAAPHADVLIVAHRGLEGLANATDLLSGTVVGQKIEVSIWRIHAADIPGGSDE
                     RRRWLFDCWKRVDDFVARVDE"
     gene            5149..6198
                     /locus_tag="VFG22_06310"
     CDS             5149..6198
                     /locus_tag="VFG22_06310"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HET6415889.1"
                     /translation="MKIDTFNGETDWAKLAAAGRRAEALGFDGFAIPEITGDPLIHAA
                     MVANATERIQARTAIAVAFPRSPMVLASQAWSIQVNSGGRFALGLGTQVKAHNERRFS
                     TPWTAPRSRMVEYVKALRAIWRCWELGERLDYQGEHYQFTLMTPEFTPAKSNLPMVPV
                     YLAAVRPKMIESAASVADGLRLHGFCTKKYLNEVVWPAIDRGLASAGRSRSELDVCGG
                     GFIMTGPDEETVMKNREWVRYRLAFYGSTPSYLPVMSLHGWDDLAQKLLHMSKTGQWK
                     QMGAEIPDEMLDEFCVYAPYDALPKAIEARFGGISDTVEFNFEDDTDLDLAEEVVARV
                     HAIESQFEAPADHYA"
     gene            complement(6195..7958)
                     /locus_tag="VFG22_06315"
     CDS             complement(6195..7958)
                     /locus_tag="VFG22_06315"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF001209.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HET6415890.1"
                     /translation="MVDDFESTDSMRSGKPLRHVQKAVFPTPMRLHHGGSLPSVEVAY
                     ETYGTLDPDGSNAVLICHAITGDSHLACHDPRDEPGWWEGLVGPGKAVDTERLFVICS
                     NVLGGCRGTTGPATINPVTGIPYGSDFPLVTVEDMVDLQKRLVDLLGIKRLRAVIGGS
                     LGGHQTLCWATRYPEQVETAVVIAASARVTAQAIAFDVVGRNAIQSDPHFHRGQYYGT
                     GDFPQTGLALARMLGHITYLSSEAMTRKFDLDRHAPRDIVTDFEKRFSVGSYLAYQGE
                     QFVDRFDANSYVTVTLAMDNFDLGDTWEKRVAAFRAAQCDWLVISFSSDWLFPPSQSR
                     ELVSIMTTLGQPVSYCEIHTDGGHDSFLLPDDIEAFSPLVAAKLGAPAPRAPRISAED
                     DRILELIPPGSSVLDLGCGEGDLLARLKERGAPLLCGVEVSTDLLAATMQHGVEAIDY
                     DLNVGLPEFDDDRFDYVVLSSTLQAVPNVERLLEDALRVGHRAIVGFTNFAHRTLREM
                     FGLEGRAPKAPGPYSYEWYNTPNRRFPSIRDMLELCEKMGVTVEDARYYDDTQGRIIG
                     EDEDPNLAAETALLVLSKKSP"
BASE COUNT         1471 a         2471 c         2675 g         1525 t
ORIGIN      
        1 gcgcaagggg ctttgctagg cgaagagccc gcgcagcgct tcgtacacgt ccggatggtt
       61 cgcgatgtgg atgtggttca tgcccgggaa cacggttccg ccggagaaat gaatgcgccg
      121 ggcgggctcg ggcgcttccc cgatggcgct cggcagtcgg accatcagat cgccgaggag
      181 ctcgccgagc gggtgctccg gatcgtgcga gatggtcgcc gccaagaaat agtatccgac
      241 gccctcgacc agaggcacgt tctcgcgtgc gtcggcgaag acttcgtcgg gatttcttcc
      301 ggcccactct tcatcgacgg tgtagccgta gcgcagatcc ttcacgccgg agcttcgact
      361 gtcgagaagc gcggcgggaa cctcggcacc ggcagcgtcg agcttgcgca gcacgtgggt
      421 gaatacattc acagcctttt cgaggggcgc gccgagatgc ggcgagccga tgcaggcgac
      481 atgacgaagc tttccgatcc agggctcgtc gtgctcgcgc gcgtagtgcg ctgcgctgcg
      541 cgccaccagg ccgcccatgc tgtgcccgac gagcgcgatc tcttcgacgg gcacgggata
      601 ggcgtcgacc acttcgctga tcagcttggc cagggcgcgt ccgttctcgg agatatgccg
      661 gcctgtgttg taccgaaggt agatcggcgt gaagccgagg tcgtctcgca ggcgtgtgcc
      721 aaaggtcacg tccgggtcgc catagtgctt ttccgaggac aagctccaga gccattcggt
      781 cgcggcgagg ctgtggacga aaacacaaag cttcggcgtc gggctaggaa aggccgccgc
      841 aaaggcttcg ggcgtggcgg gaagatgccg gccatggtgc cgaagggtca tgccgagatc
      901 gaggcggttg ttcttccggc tgagatgatc tccccagaag ccgttgatcg aagcctgcag
      961 atagtcgaca taccaggtgg cggttccgag ggcgctcgag ctcaccgggg tcgcgagctc
     1021 cagggactcg gcgtccgtct gctgctcgag gcctgcctcg gcaacatcgg cggcggcgtt
     1081 cacgccgaaa cgcgcgattc cattgagtac gcggatcgac gcaaagatgc cgccggaaat
     1141 cgcggtttga acaccggtga cgagctccgc ggtgctcttg gcgggctcga tcggcgcaaa
     1201 gcggcgtttg gatcgctcga ccacggaatc gtgcgtccgc tgaacgaggt tggtcgattc
     1261 gtcgacgagg tcgaacacca gatcgacgaa accgcgtacg cgcttcatgg atcgagtact
     1321 aggtcgccga gaggcagcgc gcaatgcacg ctgcgactca ggaccgtttt gctcaagccg
     1381 ccggccggcg acgacgacga ggccgctgcc cctctccggg tcgttctccg gatcgctgac
     1441 ggttccgcgg cgggcgggcg tcgcgacgag gtcgctcgtc ttcgcgatcg cggatttgtt
     1501 cggccgggtc gcgcggcggc aggtcgaccg aaacctccga aggagagctt cgaatcaacc
     1561 tttccacggc ccgcaaacga ccgcgttccg atgggtcgac cagcgaccat gcagcgcccg
     1621 tggcacctgc gcgaccggtt cggccgatgc ggtgtacgta gctctcgggc tcgtggggga
     1681 gctcgaagtt gaacacgtga gagacggcgt cgatgtcgat gccgcgcgcg gcgatatccg
     1741 tcgccaccaa gacccaagcg tcgccgttct tgaagttgct cagcgcgcgt tggcgcgcgt
     1801 tctgcgactt gttgccatgg atggcctctg caccgatgcc ggcgcgttcg agctttttcg
     1861 ccactcggtt ggcaccgtgc ttggtgcgcg tgaacacgat ggcgcgagaa acctcgtcgt
     1921 cgcggagcag atgctcgagc atgcgctgct tgtcgttggt tccgaccatc acgacccgtt
     1981 ggtcgatctc gctgacggtc acctccttgg gcgacacatc cacgcgaatc gggtcacgga
     2041 gaatctcgcg agcgagagac acgacttcct tcggcatggt ggcggagaac aggagcgact
     2101 gacgcgtctt gggcgtcatg gcaacgattc gcttcacatc gcgaacgaat cccatgtcga
     2161 ggagcctgtc ggcttcgtcg agcaccagga ccgaaacttc agatagatcg atcttccgtt
     2221 gccccgcgag gtccaaaagc cggcccggcg tcgcaacgac cacgtcgatg ccctgggcga
     2281 gcgccttgat ctgcgggttg gcgctcacgc caccgaaaat gtacgtgtgg cgcaggctca
     2341 ggtaccgccc atagcggccg agctcttcat gaatctggag cgccagctct cgagtcggcg
     2401 cgaggatcaa ggcccggggt cgccggggct ttgcgggcga gcgttcctcg tccaagcgtt
     2461 gcagcaaggg caacgcgaat gctgcggtct tgcctgtccc cgtctgggcg atgcccaaga
     2521 ggtctctccc ctgcaacaag tgcgggatgg cctgggactg gatcggtgtg ggctctgtgt
     2581 agcccacgtc tctcagggca cggcgaagat tttcgccgac cctgagagat tcaaagtcgt
     2641 tctgactcgt ggtcattcag tagtcccgct taccaacgat tgtcggagcg gcggtcgttt
     2701 cttgggcgct cgttggcctg gtcgactcga atcgtgcggc cgtcgagcgt ggagccgttg
     2761 agctcctcgc acgcacgaac tgcactctgc tcgtcttcaa aggtcacgaa gccgaagcct
     2821 cgcgagcgac cggtttcccg atcggtgatc actttggcct cggcaacggt gccacaagcc
     2881 tcaaaagcct gctggagctc actgtctgtt gtgttccagc taaggccgcc tacgaatagt
     2941 ttcttgctca ttctgtctct ctctgatcat tctgcgaagc atggacgacg ggacctctcc
     3001 cgagtgcctg cttcgctttt acttgccgat gtcttctacg aaccgttaac cgttgtttcc
     3061 attcacggtg cgggtctgat aggaacaggt cggcagcgtg ctggtggccc acggtgagcc
     3121 accgcgccca cgatgggcga tggcagcaag gtagcagttg ccctgacctt gtccagaagg
     3181 cggttgaggg gtgcccagga aggtgctcca atacggcgat gatgaagaac ctcagcttcc
     3241 acgtcctctc gatgatcctc actgtttcct tggttctggg ctgtcagagc aagggaccga
     3301 ccccgaagga ggggaacgcg ccgggcgagc cctcacctcg agtgggcacg accgaaacca
     3361 ccggcgccca cccgggcggt accgcagacc cgcacgcagg aatggctgcg gcgggggacc
     3421 cgcatgctgg aatggcgccc acgcagaacc cacacggtgg aacggaggtc actggcctcc
     3481 ccgacgacaa cggcatgctc gacgtagggg ccgtcgcatt caaggtgccc ggagcctgga
     3541 gcgttcgacc gccgaagtcg cagatgcgac tcgcgcaact cgaagcgcaa ggaaaggcag
     3601 ggcccgcaga gctcatcgtc tatttcttcg ggccacaagg cgcgggaaca tcggatgcga
     3661 acatcgatcg ctggataggg cagttcgcca ataccgacgg ctcccccgtc acgaacgcga
     3721 cgcgatcatc gagcaaggtt tcgggctatg acgtggcgag ggtcgaggta gccggcgagt
     3781 acggaggcgg aatggcccct ccaggacaag cccaggaagc ggtgccgggt cagcgtctgc
     3841 tcgcggccat cgtcgccagc gaaggcggac cgtactactt caagttcctc ggtccggacg
     3901 ccaccgtgca agaacatcgc gaagcgttcg acggcttaat cgcgtcgatc gttccgtcgc
     3961 cctaaggcga cgtgacgcgt caacgcagag gcgaggggtt tcgttcgcga gccttcccgg
     4021 ataaattccg gctcgagtca tgatcgggac acgagagcag tcgagtcaag tgcggcccac
     4081 ggaccttcgc cgacgctcgc gcttcgtgca ggtgctggcg ttttcccatt cgagcgcggt
     4141 ctctcccgct tggcggcggc tcatgacgac ctcgacgtac gtcctgacct gggcgggtct
     4201 gaccgcgctt attcctttgt ggatcccgat cgcgttcctg gtcggtgtcg tccggcgatg
     4261 ctcgttcgtc attctccgcc tgctgacgtt cttctggttc tatctgacga tcgagctcat
     4321 gggactgatc gcggccgggt ggatccacct cgtgacgcgc ggcaacgacc ggcggcggca
     4381 ggatctcatg tttcagctcg aatgctggtg gggtagctcg ctcttcagtt ggcttcggcg
     4441 acttctttcg ctctcggttt cgatcgaggg cgaggagcag attcttccgg ggcccgtgct
     4501 ggtgttcatc cggcacgcga gcatcatcga cacggcggtg cccgtggccc tcctgtcgac
     4561 cagaacagga ctccggctgc gatacgtttt caagcgcgag ctcctggtcg acccgtgcat
     4621 cgatgtggcc ggtcatgcct cgccaaacta tttcatcgat cgcggcggaa gtcccgagca
     4681 ggagctcgcg ggcgtccgaa agctcgccga gaacctcggc gatgaaggtg ttcttctcta
     4741 tccggagggc actcgattca ccaaacgaaa gcaggagatc gcgctcgcgc gattggccaa
     4801 ggcccatccc gacctcgtgc cgacggccac gtcctttcgt cattgcctcc cacccaagcc
     4861 aggaggcgtg cttaccttgc tcgatgccgc gccccatgcc gacgtgctga tcgtcgccca
     4921 ccgagggctc gaaggtctgg cgaacgccac ggacttgttg agcggcacgg tggtgggtca
     4981 gaagatcgag gttagcatct ggcgcatcca cgccgcggac attccggggg ggagtgacga
     5041 gcggagacga tggctgttcg actgctggaa gcgcgtagac gacttcgtcg cgcgagttga
     5101 tgagtaggac ccctgatcag caggctcgag tcgtggtaga ggggatgcat gaagatcgac
     5161 acgttcaacg gcgaaaccga ctgggccaag ctcgccgcgg cggggcggag agccgaggcc
     5221 ctggggtttg acgggttcgc gattcccgag atcaccggcg accctttgat ccacgcagcc
     5281 atggtggcga acgctacgga gaggattcag gcgcgcaccg cgatcgccgt tgccttccct
     5341 cgaagcccga tggtgctcgc aagccaggca tggtcgatcc aggtcaattc gggagggcgc
     5401 tttgcgctcg gcctcgggac gcaggtcaaa gcgcacaacg aacggcgctt cagcacgcct
     5461 tggactgcac cacgaagccg catggtcgaa tacgtgaaag cgcttcgcgc gatctggcga
     5521 tgctgggagc tcggtgagcg cttggattat cagggagagc actatcagtt cacgctgatg
     5581 acgccggagt tcacgcccgc gaagtcgaac ctgccgatgg tgcccgtcta cctcgcagcg
     5641 gtccggccga aaatgatcga atcagcagcg agcgtggcgg atgggctcag gctccacgga
     5701 ttctgtacta agaagtactt gaacgaagtg gtttggccgg cgatcgatcg cggcctcgcc
     5761 tcggcagggc gctcgcgaag cgagctcgac gtttgcgggg gcggtttcat catgaccggt
     5821 ccggacgaag agacggtgat gaagaaccgg gagtgggtcc gctaccgatt ggcgttctac
     5881 ggatcgaccc catcgtatct gcccgtcatg tcgcttcacg gttgggatga cctggcgcag
     5941 aagctccttc acatgtcgaa gaccgggcag tggaagcaga tgggcgcgga gatcccagac
     6001 gagatgctcg acgaattctg cgtttacgcg ccatacgatg cccttccaaa agcgatcgag
     6061 gcgcgcttcg gcgggatctc ggacaccgtg gaattcaact tcgaagacga tacggacctc
     6121 gacttggcag aggaggtcgt ggcccgggtg cacgcgatcg agagccagtt cgaggctccg
     6181 gccgatcact acgcctaggg actcttcttg ctgaggacga ggagagcagt ttcggcggcg
     6241 aggttcgggt cctcgtcttc gccgatgatg cgaccctggg tgtcgtcgta gtagcgggcg
     6301 tcctcgaccg tgacgcccat cttttcgcag agctcgagca tgtcgcggat cgatgggaat
     6361 cggcggttcg gggtgttgta ccattcgtac gaatatgggc ctggggcctt gggggcccgg
     6421 ccttcgaggc cgaacatctc tcgcagcgtt cgatgggcga aattggtgaa gccgacgatc
     6481 gcgcggtgac cgacgcgaag tgcgtcttcg agcaagcgct cgacgtttgg aacggcctga
     6541 agcgtcgacg agagcacgac gtaatcgaat cggtcgtcgt cgaactcggg caaaccgacg
     6601 ttcaagtcgt agtcgatcgc ctcgacgccg tgctgcatcg ttgcggcgag cagatcggtg
     6661 ctcacctcga ctccgcagag aagcggcgcg ccacgctctt tcaagcgcgc cagaaggtcg
     6721 ccctccccac atccgagatc gagcacggag ctgcccggcg gaatcagctc gagaatgcgg
     6781 tcatcctccg cgctgattcg cggagcgcgt ggtgcaggcg cgccgagctt cgcggccacg
     6841 agtgggctga aggcctcgat gtcatcgggc aggagaaaac tgtcgtgacc gccgtcggtg
     6901 tggatctcgc agtacgaaac cggttggcca agagtggtca tgatcgagac cagctcgcgc
     6961 gactgactcg gcgggaagag ccaatcgctc gagaacgaga tgaccagcca atcgcattgg
     7021 gcagcgcgaa acgctgcgac gcgtttctcc cacgtatctc cgaggtcgaa attgtccatc
     7081 gcgagggtca cggttacgta tgagttggca tcgaagcgat cgacaaactg ctcgccttga
     7141 taggccaagt acgagccgac cgagaagcgc ttctcgaagt ccgtcacgat gtccctcggc
     7201 gcatgccggt cgaggtcgaa tttccgcgtc atcgcctcag aggacagata ggtgatgtgg
     7261 ccgagcatcc gcgcaagcgc gaggcccgtc tggggaaagt ccccggtccc gtagtactgc
     7321 cctcgatgaa aatgagggtc gctctggatg gcgttgcggc cgaccacatc gaaggcgatc
     7381 gcctgagccg tgactcgtgc cgaggcagcg atcacgaccg ctgtttccac ctgctcggga
     7441 tagcgggtgg cccagcagag cgtctgatga ccaccgagcg atccaccgat gactgcccga
     7501 aggcgcttga tgccgagcag atcgacgagc ctcttctgca agtcgaccat gtcctcgacg
     7561 gtgacgagtg ggaaatcgct gccataggga atcccggtca ccgggttgat cgtggccggc
     7621 ccggtcgtgc cccggcagcc accgagaacg ttggaacaga tcacgaacaa gcgctccgtg
     7681 tcgacggctt tgcccggacc gaccaatcct tcccaccagc ctggctcatc tcgtgggtca
     7741 tggcaggcga ggtgtgagtc gccggtgatc gcatgacaga tcagcacggc gttgctgcca
     7801 tccgggtcga gggtgccata ggtctcgtac gcaacctcca cactcggcag agatccgccg
     7861 tggtggaggc gcatcggagt cggaaagacg gccttctgta cgtgacgaag cggctttccg
     7921 cttcgcatcg aatccgtgct ttcgaaatcg tcgaccacga gcgtcggtta gcctacctca
     7981 cggcggcggc aagcgcctgg tcgatgtccg caatgatgtc tttcacatct tcgatgccga
     8041 ccgaaacgcg cacgtactcg ggcacgacac ccgcttgctg ctgctcggct gggctcaact
     8101 gctggtgcgt ggtcgaggcg ggatggatca cgagggtctt gg
//
LOCUS       DASRZF010000139         7930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4221735, whole genome shotgun sequence.
ACCESSION   DASRZF010000139 DASRZF010000000
VERSION     DASRZF010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 7930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7930
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4221735"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..1037
                     /locus_tag="VFG22_06320"
     CDS             93..1037
                     /locus_tag="VFG22_06320"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET6415891.1"
                     /translation="MKSPIVHFALLGLVLFVGARWLFPSEPTERAPDTRIVVSESRVA
                     ALVSQYEATSGMVATDDFRAELIQRFAEEEMLCREAQRWGLTENNQAIDLRLRQKMAF
                     VSDEEHSDEELERQAKALGLGSDDAVIRSMLVHNMRLLLSTRGEQEPTDEEVEAYYER
                     ERLRFSRPARMTGWHVFFSKDLRHSSAMAAARAAKEELDAEALEPEQAVELGDVSPSG
                     ASFKGQLAQQLASRFGPEFAKAAERLPEGQWSDPIETPFGAHLLLVQERTQPQTPPLS
                     EIRARVAAAYRSSQRQVRLDAAMEELRARYEVTSSSAE"
     gene            complement(1015..2901)
                     /locus_tag="VFG22_06325"
     CDS             complement(1015..2901)
                     /locus_tag="VFG22_06325"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HET6415892.1"
                     /translation="MDVELLYNPSLTVAIALAAGMVTQSIAYHLRIPSIVLLLAVGVL
                     LGPEVAGIVDPESLGSSLHALVGMGVAIILLEGGLQLELRRLRREQVVIRRLVTVGAV
                     ITAIGGAVAAHSALGWDWRLASLFGALVIVTGPTVVTPLLRRLRIRGAVPTVLEAEGV
                     LIDAIGAITAAVVLEVVLRPSDESFLLAAPQVALRLGFGVSAGLLAGGVLAGLLRFRG
                     LIPEAYHKVLTLSVAVLIYELANSVVHESGLAAATITGMVLANSPIRKSRDLIEFKEQ
                     LTVLLIGMLFILLAANTRVADIIELGWPGAITVAALVVLVRPIEVFLCSMGTTLSWRE
                     RVLIAWIAPRGIVAAAVASLFAIRLESEGIAGGAQLQALVFATIAATVIWSGLTGAAI
                     AQLLGLRRRSDAGWLILGTNELAIELAGLLQTPRQEVVCLDEDPHRVQAAEEAGIRVL
                     LGNALETRLLMRAEVELRTGAVAVTESPEVNLLFINKVHQEAKEQRCLVAIDTWSTGV
                     TPEIVETKDAEVLFGGTTDVAQWSQRIDRGEAQVEWWSCRAWRPKGIFTEGESRPAYL
                     PMVRRRWRRRIAVGTRTRFWRNDRVAVLVDLARQEEAHERLQHSGFVRSEDPASAVTP
                     RSSS"
     gene            complement(3130..3411)
                     /locus_tag="VFG22_06330"
     CDS             complement(3130..3411)
                     /locus_tag="VFG22_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415893.1"
                     /translation="MAKNLVMTLVGAAFAAALWLAPSQASAANCNFNKNEKIAMVSWI
                     IPIAGAVMTPLACKRDLFNKKSKGDAALPAEHRKQADIRFLDRRRHRMM"
     gene            3582..3770
                     /locus_tag="VFG22_06335"
     CDS             3582..3770
                     /locus_tag="VFG22_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415894.1"
                     /translation="MQVQKFLAVPTQVLQFGALACNEEEAGRHVLIARRGWHDDLDDL
                     EKLEEFALAKLEVDEQGA"
     gene            complement(5126..6097)
                     /locus_tag="VFG22_06340"
     CDS             complement(5126..6097)
                     /locus_tag="VFG22_06340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HET6415895.1"
                     /translation="MLIDSPLLLGGLVLLMGGAESLVRGASGIALIARVPPAVVGLTI
                     VAAGTSMPELVVSVQASLQGSPGLSMGNVIGSNIFNIGCILGLTAVLRPLRILGNTVR
                     FEWPVMMLAAVLIHLLARDLVVDRWESGFLFMCMVAFTAYAVWLGRKGVAAVEQTEFA
                     QLTTASFGRSGKAATGLNVLAVALGVGLLGTGSTALVRGAVGMASSFGVSDRVIGLTI
                     VAAGTSMPELVTSLMAARRGQDDIAVGNVVGSNIFNVLGIIGVVGSIRPLPVPREIVA
                     WDDWWMLGASALLFPLMRTDMTIKRIEGALLLAGFVTYVALLILRTT"
     gene            7201..7896
                     /locus_tag="VFG22_06345"
     CDS             7201..7896
                     /locus_tag="VFG22_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HET6415896.1"
                     /translation="MTDNHKEAHRTERIGWLRAAVLGANDGIVSTASLVVGVAAADAT
                     HESIMIAGVAGLVAGAMSMAAGEYVSVSSQADTENADLDRERTELATDGEFELSELTA
                     IYVERGLDEALARQVAKRLTAHDALGAHARDELGISAMMSARPVQAALASAATFAVGA
                     AAPLLTAFMSPTERIPMVVSFTSLGFLALLGGVSARTGGAPVGRAVARVTFWGALAMA
                     ATAGIGALFGVAA"
BASE COUNT         1557 a         2484 c         2441 g         1448 t
ORIGIN      
        1 ccggtcatcg aagatcagag ggtgatcaaa gagcgagcgt ggagctcgcc gatttggtac
       61 cgccctgaaa cgctttgaag ctacctaagc tcatgaagtc gcctatcgtg cactttgcgc
      121 tgctcgggct cgtcctcttc gtcggcgcca gatggctttt tccaagcgag ccgactgaac
      181 gagcgcccga tactcgcatc gtcgtttctg aatcacgggt tgcggcattg gtcagccagt
      241 acgaggcgac gagcggcatg gtggccactg acgactttcg agccgagctc attcagcgct
      301 ttgccgagga agaaatgctc tgccgtgagg cccagcgctg ggggctaacg gagaacaacc
      361 aggccatcga tctcaggctc cgccagaaga tggcgttcgt gtccgacgaa gagcactcgg
      421 atgaagagct cgagcgccag gccaaagcgt tgggcctcgg ttcagacgac gccgtgatcc
      481 gcagcatgct cgtccacaac atgcgcttgc tcttgtctac aaggggggag caggagccaa
      541 ccgacgagga ggtcgaggcg tactacgaac gcgagcgctt gcggttttcg cgtccagctc
      601 gaatgacggg atggcatgtc ttcttctcca aagacctccg gcattcgtcg gctatggcag
      661 cggcccgtgc ggccaaagaa gagctcgacg cggaggcgct cgagcccgaa caggccgtcg
      721 agctggggga cgtgtctccg tccggagcga gcttcaaggg acagctcgcc cagcagcttg
      781 cgtcccgctt tggcccagaa ttcgccaagg ctgccgagcg ccttcccgag gggcagtggt
      841 ccgacccgat cgaaactccg ttcggagctc acctcctgct cgtccaagag cgcacgcagc
      901 cgcagactcc gccgctttca gagatccgcg cccgcgtcgc ggccgcgtac cgatcgagcc
      961 agcgacaagt gcgcttggac gccgcgatgg aagagctgcg cgcgcgctac gaagtcacga
     1021 gctcgagcgc ggagtgaccg cggacgctgg gtcctcgctc cgcacgaacc cggagtgctg
     1081 caatcgttcg tgcgcttctt cctgccgggc caggtcgacg agaaccgcca ctctgtcgtt
     1141 gcgccaaaat cgtgttcggg tccccaccgc gatccgtctc ctccatcttc gccggaccat
     1201 cggcaggtac gctggacggc tctcgccctc ggtgaagatt cccttcggtc gccaggctcg
     1261 gcaagaccac cattcgacct gcgcctcacc gcgatcgatg cgctgactcc actgcgccac
     1321 atcggtggtt cccccgaaaa gcacctcagc gtccttggtt tccacgatct ccggcgtgac
     1381 tccggtcgac caagtgtcta tcgcgacgag acaacgttgc tccttggcct cttgatgaac
     1441 tttattgatg aacaagagat tgacctcggg gctttccgtg acggctaccg cgccggtacg
     1501 gagctcgact tccgcacgca tcaggagccg agtttcgagg gcgttgccga gtagtaccct
     1561 gatcccggct tcctccgcgg cctgcactcg gtgcgggtcc tcgtccagac acacgacctc
     1621 ctgccgcggt gtttgcaaca gccctgctag ctcgatcgca agctcattcg ttccgaggat
     1681 caaccaaccc gcgtccgagc gtcggcggag accgaggagt tgagcgatcg ccgctcccgt
     1741 gagacccgac cagatgaccg tagcggcgat cgtcgcgaac accagcgcct gaagctgcgc
     1801 gccccctgca atgccctcgc tttccagccg aatcgcgaac aaggatgcga ctgctgcggc
     1861 gacgataccg cggggtgcga tccacgcgat gaggactcgc tctcgccacg acagcgtggt
     1921 gcccatcgag cacaggaaca cttcgatcgg gcgaaccaat acgacgagcg cggcgaccgt
     1981 gatcgctccc ggccagccga gctcgatgat gtcagccacc cgagtgttcg ccgcgagcag
     2041 gatgaagagc atcccgatga gcaacacggt cagttgctct ttgaactcga tcaagtcacg
     2101 gctctttctt atgggggagt tcgcgaggac catgccggtg atggtggctg ctgcaaggcc
     2161 gctctcgtgc acgaccgaat tggctagctc gtagatcagt accgcgaccg agagcgtcag
     2221 gaccttgtgg tatgcctcgg gaatcagacc ccggaatcga agcaaacccg cgagcacgcc
     2281 accggcgagc agccccgccg agacgccgaa gccaagccgc aaggcgacct gaggcgcggc
     2341 caggaggaag ctctcgtccg aaggccgcaa cacgacttcg aggacgaccg ctgccgtaat
     2401 ggcgccgatc gcgtcgatga gcacgccttc ggcctcgaga accgtcggga ccgcaccgcg
     2461 tattcgcaag cgccgaagga gcggagtcac taccgttggc ccggtcacga tcaccagtgc
     2521 gccgaacaga gaagccaggc gccagtccca gccgagcgcg ctgtgcgccg cgaccgctcc
     2581 gccgatcgcg gtgatcaccg cacccactgt cacgaggcgc cgaatgacga cttgctctcg
     2641 gcgaagtcgg cgcaactcga gctgaagccc accttcgaga agaatgatcg caacgcccat
     2701 accgacgagc gcgtgcaacg aggaaccgag cgactcggga tcgacgatgc ctgcaacctc
     2761 gggccctagc agtaccccaa cggcaaggag caagacgatg ctcggtattc ggagatggta
     2821 cgcgatcgac tgcgtgacca tgcccgcagc aagcgcgatc gccacggtga gcgaaggatt
     2881 gtataagagt tcgacgtcca tcgagaaccg cccgcggcga gggaacgtag caccctcaat
     2941 agggccacgc ttcgtgactc atggcttcat ccgtcgtggc acttccgccc agcatcgcag
     3001 acggcgcgca gagcttggct acgaagtcga gctcgggatg gggctaaaga cggtggttga
     3061 cgcatgccgg gatgagcttc tggacaaacg aagacaccag cggagagact actcgttctg
     3121 cgcgcgccac tacatcatgc gatgccgacg acggtcgaga aaccgaatat cagcctgttt
     3181 gcggtgctcg gcaggtagcg ccgcatcgcc tttgctcttc ttgttgaaga ggtcccgctt
     3241 gcacgccaag ggagtcatca ccgcgccagc gatggggata atccaagaga ccatcgcgat
     3301 cttctcgttc ttgttgaagt tgcagttggc tgccgaggcc tgcgatgggg cgagccacaa
     3361 ggcggccgcg aacgcagcgc caaccagtgt catgacaaga ttcttcgcca ttctttcttt
     3421 cccttcgtgg catcgccacc gttagagtct gcttcgcccc gcaacctcaa ggtccattac
     3481 gtggtcatca acctagtgac acgctccatg tgcttatcaa gagctttggc ggcctaagca
     3541 tcgatgcaag catattggtt catcgttatc cttaggacgg tgtgcaggtc cagaaattct
     3601 tagctgttcc aacgcaagtc ctccaatttg gagcgctcgc ctgtaacgag gaagaagccg
     3661 gccggcatgt tctgatcgcc cgtcgaggct ggcatgacga tctcgacgat cttgaaaagc
     3721 tcgaggaatt cgcccttgct aaactggaag tcgatgagca aggcgcttga ggacggctgg
     3781 aagcgccacg agtccgggca gcggcgcgca tggatgaagc tgaccccggc acagcgcctc
     3841 gcatggctcg gggacgccaa gcggggccat tcatcgccga cgccgcaggc gagggctatc
     3901 gacgcgcggc gagcggagag cccaccccat acaatggctt cttctaccgg atgctgtact
     3961 cgcaggaccg agcgcgcagg ccggggcgaa agactacttc gtggctcggc gtatggtcgg
     4021 cggctttgcg ttgatcgcat ggcgcgcaca gtgaggcatc agcggagtca agacgttcat
     4081 cgtcaaccaa gacgacgtcg tctatgagaa agactttggc gacgacactg cgggtctagc
     4141 gaaccaaatt gtcgcgttcg atccggacaa atcttggagc cgcgtggagg aaggcgaagg
     4201 ccgctgagcg gaccgcgacg gccgctaagc tgtgctagcc cgcttcgagc ccgaccgcga
     4261 ctgagtaggc accggcgtaa tccagcgggt gcgccgggga gctaacttgc caaagagcaa
     4321 gcccggatcc atctttggaa gatgcggtcg gtgtctcggc tgtttgtgtt ggcggtagtt
     4381 atcgttggct cggccggtta tgcggttttg tgcctctcgc cgcaccagcc actcccccga
     4441 atcgaacgcg ttgccgcgaa ggtgaaatcg gccctgctgt tggctgcccc gcaagcaacg
     4501 ccagcctcgg aggtccgtct cacacagaaa tcggacgact tgggagcttt cgagcgcgca
     4561 gagaccgcgt tcgatcgaca tgcgagtcgt gtgttgactc cgtcggcgtc tcaccgacgc
     4621 caccgtgtga tttcggacca gcgcccagcg atacgactga cttgccgggg cacaagagcc
     4681 gcccattcga gcgacgaacc tcccgaacga cctcgttctc tatcctgaac gtccgggtct
     4741 gttcgaagaa tcgccatccg agttggctgg cgaatggagg aatttgtgat gcgtacgaat
     4801 ttgaaaagca gctcagttgc cagcacagtg aatgtccccc tggcgccaat gccagaagcc
     4861 gctgtcgttc caagtgagcc ggttgcgccg gcaccccaac ctacagcggc acgcccgcag
     4921 ggccggggct gggccgaaga ctggagcgac ccctccaagc tacctcaggt gagcgcactc
     4981 tggcccctgt tgtggttgac gatcccgctc gtcctgcttg ttctatacgg ctacttctcg
     5041 cggggttagc gacgtgggtc gtgcgcggcc ggaacgacca caccagcatt cttatccgga
     5101 tgtccatgtg aaggactgag ttggctcaag tagtccgaag gatcagaagc gccacgtacg
     5161 tgacaaaccc agctagaagc aacgcacctt cgatgcgctt gatcgtcatg tccgtcctca
     5221 tcaacgggaa caagagcgcg gatgcaccca gcatccacca gtcgtcccag gcgacgatct
     5281 cccgcgggac tggcagtgga cgaatcgagc ctacgacgcc gatgatccca agtacgttga
     5341 agatgttcga gcccaccacg ttgccgaccg caatgtcgtc ttgtcctctt cgtgccgcca
     5401 taagagaggt cacgagctct ggcatgctcg tacctgccgc cacgatggtg aggccaatca
     5461 cccgatccga gacgccgaac gagctggcca tcccgaccgc tccgcgcaca agggctgtgg
     5521 agcctgttcc gagcagcccg acccccaagg cgaccgccag gacgttgagg ccggtcgccg
     5581 cttttcccga tctaccaaag gacgcggtcg tcagttgcgc gaattcagtt tgctcgacgg
     5641 cggccactcc ttttcgtccg agccagacgg catacgccgt gaacgcgacc atgcacatga
     5701 acaggaaacc gctctcccat cgatcgacga ccaaatcgcg cgccagcaga tggatcagga
     5761 ccgccgcgag catcatgacg ggccattcga accggaccgt gttgcccaag atacggaggg
     5821 ggcgcaacac cgcagtcaat cccagaatgc aaccgatgtt gaagatgttg gagccgatca
     5881 cgtttcccat ggagaggccc gggcttccct ggagggaagc ctgcacagac acgacgagct
     5941 cgggcatcga ggtcccggct gctacgatgg tgagtcccac gaccgccggt gggactcgcg
     6001 caatcagcgc gatgccgctt gcgccccgta cgagagactc ggcgccgccc atgagcaaga
     6061 cgaggcctcc gagcaagagt ggtgaatcga tgagcatttc gagcgcccca gtttgattgt
     6121 ggcgtcaagc atgcagcggg ggcacgcctg ccggcaacgc cacatagctt gcgcgagtcg
     6181 tctcgcgcaa atcagccgaa gatcctcggc gcggcaccct caggtcaacg aaccgccgca
     6241 agcaacgaca ggccggttcg gcaagcgagc gctctgccag ccacttgtaa tcagcacgct
     6301 tcattcgacc tccctttttc gcctatcccc cgcctaattc ggccagtcag caccacttgg
     6361 tgtaggctct tgcgtgtgtc atcgcgttcg atggcgtggg aggttgcgcg atgacgaaac
     6421 tgcagttctt gtgcgcggtt gtgtttggat cgatgctggc tatcaccggt tgcggagaga
     6481 caactggtgg ctccgctggc tcaggaggct cgggcggttc cggcggaggc atggtgaccg
     6541 gttcgtgcca gatcatctgc aatgccgact gtgagctttt cggccccgac ccagctagcg
     6601 ccacgtgcgc ctcggactgc gtggcagaaa acttggaggg atgtgtctcc gagacgacag
     6661 cactagtggc atgcgccgat caagcgcagg gcggcgactg caatatagac ccaacggatt
     6721 cgtgccaatc gcaggttgct gccttcaatg cctgtgactc agggagcggc ggcacggcgg
     6781 gcgattgccc gaacggcact tatctctgta gcgctgccgc cacgtgcttc gaaacgattt
     6841 atggcgccgc cggagcacaa gcatctccaa cgtgcgtggc tgacgtctgt tcctttttcg
     6901 gcacgaatga ttgcgtggct cagcttgcgg ccgagcaagc atgtgcggct caagacaact
     6961 gcagtattga caactgccag gctgaatccg acgccgctga cgcgtgcacc ggcatctagc
     7021 tacgagaatg caccaagtcc aacgaccccc tacgccgagc gcaagccgat gaccactacc
     7081 ctgtaccagt tcgcgctctg tcgatgtcac tcaagtgtcg aatactttcg ccaatccggc
     7141 taagatccgc agtggatggc atctgcatga tggcgcgtgt ccgtcccgag gacgatatcg
     7201 atgactgaca atcacaaaga agcgcatcgt acggaacgga ttgggtggct tcgcgcagcg
     7261 gtgctggggg caaacgacgg tatcgtctca accgcgagct tggtcgtggg ggtcgccgct
     7321 gccgacgcca cccatgaaag catcatgatc gccggcgtcg ctggcctggt tgccggcgca
     7381 atgtcgatgg cagccggaga atatgtgtcg gtgagctccc aggcggacac ggaaaacgct
     7441 gacctcgacc gcgagcgaac ggagctcgcg acggatggcg aattcgagct ctctgagctg
     7501 actgctattt acgttgaacg cggattggat gaagcgcttg cgcgacaggt tgccaaacga
     7561 ttgacggcac acgacgcgct gggagcacac gcgcgcgacg agctaggcat ctccgcaatg
     7621 atgagcgccc gtcctgttca ggcagcgctc gcttcggctg cgacatttgc ggtgggcgcc
     7681 gcggcgccgc tcctgaccgc cttcatgtcg ccgacggagc ggatcccgat ggtcgtctcc
     7741 ttcacgtctt tgggttttct tgctttgctt ggtggggtct ctgcgcgtac cggtggtgcc
     7801 ccggtcggta gagccgtggc tcgggtcact ttttggggcg cactggccat ggcagcgacc
     7861 gctgggatcg gcgccctttt cggagttgcc gcgtaaggcc tcggagatcg ggagttgcgg
     7921 gggcctgcta
//
LOCUS       DASRZF010000140         5767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_438773, whole genome shotgun sequence.
ACCESSION   DASRZF010000140 DASRZF010000000
VERSION     DASRZF010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 5767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5767
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438773"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..568)
                     /gene="dtd"
                     /locus_tag="VFG22_06350"
     CDS             complement(119..568)
                     /gene="dtd"
                     /locus_tag="VFG22_06350"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HET6415897.1"
                     /translation="MRAVIQRVNEATVRVDGEQVGAIDRGLLVFLGAADGDEDRDVRY
                     AVDKIAGLRVFPNEEGRMSLSVGDIGGSILVVSQFTLFGDVRRGRRPSFDGAAKPEDA
                     ERLYEEVVERLRGSGLRVETGTFRAMMRVEAVVDGPVTIQIDSRKLY"
     gene            635..1255
                     /gene="coaE"
                     /locus_tag="VFG22_06355"
     CDS             635..1255
                     /gene="coaE"
                     /locus_tag="VFG22_06355"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736397.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HET6415898.1"
                     /translation="MKPVVGLTGGIACGKTTVASMFGDLGIPVVDADALAREVVEPGT
                     PGLQRIVDEFGKGVLDQGGRLDRKKVGDLVFGDEDAREKLNAIMHPMIGAAGAMHMAA
                     LQESDAPYLLYEAALLVETRSYEAFSALIVVSAEESLQRLRLIARDGFSVSEANARIA
                     SQLPLARKIAVADYVVTNNGDLEATRRQVADVHEKLVAKFARKESK"
     gene            1255..2313
                     /locus_tag="VFG22_06360"
     CDS             1255..2313
                     /locus_tag="VFG22_06360"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6415899.1"
                     /translation="MGALGRSVLVTGFPGRQPATHLVRELAAQGELSVCCVVTEARQA
                     RAIELLSALPPSVRHRVTLSVGDPRSMDLGLSGEEFAMLAERVEVIHHCASITDPAGT
                     SEEAAGNLRAAAEILELAEAAPRLKRLVCWSSATVSGNREGFVKETDLDDAMGFRNPV
                     EESLYRMERVFRESADELPIVILRPALIVGDSVTGEVEDLNGLYLLIRFLLSAPEDYR
                     IPTPSRTGVRISAVPVDYAVKAGLRIAAHESSIGRTFHIVDPKPVTVQYALRLFAEAT
                     GRPAPREHDPLNLATALMRAPGLQRFRSTTRAFLDQLKTDVIYDDRNARELLTGTGVL
                     CPDLESYVDTMVARAQRD"
     gene            complement(2310..3686)
                     /locus_tag="VFG22_06365"
     CDS             complement(2310..3686)
                     /locus_tag="VFG22_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HET6415900.1"
                     /translation="MRDAQQEAEALIELGRSVTERARRAGADVAEAGVHSGSHLSVKA
                     RMGEPELVEEAGSRALGLRVMVGQRVASTYTSDMSESGQRTLIEDALELARLSEADEF
                     AGPPDPSDLSSESQWMDLDAFDDGVSQIGADEALTRALRGERAAFDFDARVTNSEGAS
                     FTRARGVSALVTSGGFAGTDHGTYASIVVNPVADDEGGKKRSGYHWSASRHYSDLEDS
                     RVVGEEAARRTIAQLGAKKLSTQELPVVFDKDAARSILGLFAGCILGGSIWRKSSYLV
                     DRVGTRVASDLVDIVDDPLMPRAPGSRAFDGEGLLSRRNTVVESGLLKTYLMDTYSAR
                     KLGLRSTGSASRSPSGGISSSASNLILKPSAQSRDALLADTKHGLYVTGMMGFGFNAV
                     TGDFSRGASGFLIRNGELTDPVSEVTISLNLDQLLQRIDAVANDLDQRTAIASPTFRV
                     SSMTLAGS"
     gene            complement(3683..5128)
                     /locus_tag="VFG22_06370"
     CDS             complement(3683..5128)
                     /locus_tag="VFG22_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HET6415901.1"
                     /translation="MAARYHAPFAAQGSHTVDADIANRLLRIALAKGGDYADLFFEYS
                     VSGSFGYDEQILKSARRAVSMGLGVRVMKGDATGYAYCEDFSFDTMKQAAETAAEIAA
                     GGQPVAPREVSMRSTPSRYPVDVESFDVEGEAKKAILERADKAARAADPRIIRVDASF
                     AESMREILIANSLGHMAHDRQPLIRFGVHVIAEDGGNRQSGSSGGGGRIGLEYFEDKT
                     PEWHAEQAVQQALVMLDAREAPAGEMDVVLAPGDSGILLHEAVGHGLEADFNRKKTSN
                     YSDRIGQEVASALCTVVDDASLQGSRGSINVDDEGQLPSRSVLIENGVLKGYMQDRHS
                     ADFFGTTSDGHGRRQSFRSHPMPRMTNTMLLGGVDEPEDVIRSVRRGVYAKKFGGGQV
                     DISNGDFVFSLTEGYLIEDGKLTAPLKGVNLIGNGPEAMRNVVMLGHDFEVSDGIWTC
                     GKEGQSVPVGVGCPTIKIASMTVGGTQVAGG"
     gene            complement(5173..>5767)
                     /locus_tag="VFG22_06375"
     CDS             complement(5173..>5767)
                     /locus_tag="VFG22_06375"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HET6415902.1"
                     /translation="GAYGCFSFFPSKNLGGFGDAGLVTTQDAGLASKARVMRAHGSQP
                     KYYHAVIGGNFRIDALQAALLRVKLPELEGWTRRRQENANYYDAAFAESGLDEGLLRP
                     PARRFEGHIYNQYVIRTSRRDALQAHLQEENIATAIYYPVPLHRQECFASLGYREGSL
                     PRSEQASREVLALPIFAGLGESRQNRIIEAVIGFLRR"
BASE COUNT         1118 a         1849 c         1701 g         1099 t
ORIGIN      
        1 catgcccaaa ggccaatcac gagcacgagc acgacgaagg ccgcgaacac ccatgcgcgc
       61 cgctcgcttg agaaccgcag catcgaggcg agattatgcc gcgtctagat tgggaatcct
      121 aataaagctt tcggctgtcg atctggatcg tcaccggtcc atccacgacg gcttcgaccc
      181 gcatcatcgc gcgaaacgtg cctgtttcga cgcgcaaccc gctcccacgc aaacgctcga
      241 cgacctcctc gtagaggcgc tcggcatctt cgggtttggc tgcgccatcg aacgaagggc
      301 gccttcctct cctcacgtcc ccgaacagcg tgaattgcga aacgacgaga atcgaaccgc
      361 caatgtcgcc gacggacaaa gacatgcgtc cttcctcgtt tgggaacaca cgcaagcccg
      421 cgattttgtc taccgcgtaa cgaacgtctc tgtcttcatc tccatcggcc gctcctagaa
      481 aaaccaggag gccacggtcg atggctccca cctgttctcc gtcgactctt actgtcgctt
      541 cgttgacccg ctgtatcacg gctcgcatgc ggctcgcatt agcacaggcc gaatcgtgtt
      601 gctcgcagga ggctatgttc aatactgccg ccgtatgaaa ccggtggtgg gtctgacggg
      661 aggaatagcg tgcggcaaga cgaccgtcgc gagcatgttc ggcgacctcg gcattcctgt
      721 cgtcgacgcg gacgcgctgg cccgcgaagt ggtcgagcca gggacacccg gcttacaacg
      781 aatcgttgac gagttcggca agggagtgct cgaccagggg ggccgcctcg atcgaaagaa
      841 agtcggcgac ttggtgttcg gtgacgaaga tgcgcgcgag aagctcaatg caatcatgca
      901 tccgatgatc ggtgcggctg gcgccatgca catggcagcg cttcaagaga gcgacgcgcc
      961 atatctcctc tacgaagccg ctttgctcgt cgaaacgcgc tcctatgagg cgttttccgc
     1021 gctcatcgtc gtgagcgccg aagagtccct tcagcggctt cgcctgatcg cccgggatgg
     1081 tttcagtgtc agcgaggcga atgctcgaat cgcttcacag cttcctctcg cccgaaagat
     1141 cgccgtcgcg gactacgtcg tgaccaacaa tggcgatctc gaggcgacgc gtcggcaggt
     1201 cgccgatgta cacgagaagc tcgtggcgaa gtttgctcgg aaggagtcga aatagtgggg
     1261 gctctaggac gatcggttct cgtcactggt ttccccggac ggcaacccgc cacgcacttg
     1321 gtgcgcgagc tcgcggcaca aggagagcta tcggtctgct gcgtcgtcac cgaagcacga
     1381 caggcgcgcg ctatcgagtt gctgagcgcg cttccgccgt cggtgcgaca tcgggtaacg
     1441 ctctcggtcg gcgatcctcg ctccatggat ctcggactga gcggagaaga gttcgcgatg
     1501 ctcgccgaac gcgtcgaggt gattcaccac tgcgcatcga tcaccgatcc agcaggcacg
     1561 agcgaagaag ccgcggggaa tctcagagcg gccgccgaga ttctggagct cgcagaagcg
     1621 gcacctcgac tgaaacgcct cgtctgctgg tcgagcgcca cggtgtcggg taaccgtgag
     1681 ggcttcgtca aggaaactga cctcgacgat gcaatggggt ttcgcaaccc cgtcgaggaa
     1741 tcgctctatc gcatggagcg cgtgttccgc gaatcggcag acgagctacc gatcgtcatc
     1801 ctgcgcccgg ccctcatcgt gggcgactcc gttaccggtg aggtggagga cctgaacggt
     1861 ctctatctgt tgatccgatt cctgctgagc gcaccggaag actatcgaat cccaacgccg
     1921 agtcgtaccg gagtgcgcat cagcgcggtt ccggtggact atgcagtgaa ggcaggcctc
     1981 cgcatcgcag cgcatgagag ctcgatcggg cgtaccttcc acatcgtcga tccgaagccg
     2041 gtcaccgttc aatatgccct tcgcctcttt gccgaagcca ccggccgccc tgcccctcgg
     2101 gagcacgatc ccctgaatct cgcgaccgca ctgatgcgcg ctccagggtt gcagcggttt
     2161 cgaagcacca cccgcgcatt cctcgatcag ctgaagaccg acgtgatcta cgacgaccgg
     2221 aacgcgcggg agctactcac cggcacagga gttctctgcc ccgacctcga aagctacgtc
     2281 gacaccatgg tcgcccgcgc gcagcgggac taactgccag ccaatgtcat cgacgacacc
     2341 ctgaacgtcg gtgaagcaat cgccgtgcgc tgatccaggt cgttggcaac agcgtcgatt
     2401 cgctggagca gctgatccaa gttgagagaa atcgtgactt ctgaaaccgg gtcggtgagc
     2461 tcgccatttc gaatcaagaa gcctgacgcg ccgcggctga aatctcctgt cacagcgttg
     2521 aatccgaacc ccatcattcc ggtcacatac agcccatgct tggtatccgc gaggagcgcg
     2581 tcgcgagatt gcgcgctggg cttcagaatg agattgctgg ccgaggacga gatcccgccc
     2641 gaaggtgagc gagacgcgct cccggtgctc cgcaggccca acttgcgggc gctgtacgtg
     2701 tccatcaagt acgtcttgag tagaccggat tcgacgaccg tgtttctacg agacagaagt
     2761 ccttcgccgt cgaacgcacg ggagcctggc gcacgcggca tcaatggatc gtccacgatg
     2821 tcgacgagat cgctggcaac gcgagttcca acccggtcga cgaggtatga gctcttgcgc
     2881 cagatcgagc ctccgaggat gcatccggcg aaaagaccga gaatggaccg agccgcgtcc
     2941 ttgtcgaaga cgacaggaag ctcttgggtg gataatttct ttgcgccgag ctgtgcgatc
     3001 gtgcggcgcg cagcctcctc cccaaccacc cggctatcct cgagatcgga gtagtggcgc
     3061 gacgccgacc aatggtatcc gcttcgcttc ttgccgcctt cgtcatcggc caccggattg
     3121 actacgattg acgcgtaagt tccatggtcg gttccagcga atccgccgct ggtcaccagc
     3181 gcgctcacgc cgcgggcgcg tgtgaacgaa gcaccttcgc tgttggtgac ccttgcatca
     3241 aaatcgaatg ccgcgcgctc ccctcggagc gcacgagtca aggcttcgtc cgccccgatc
     3301 tggctgacgc catcatcgaa cgcgtccaag tccatccact gcgactcgga cgagagatcg
     3361 gacggatcag gaggcccggc gaactcgtct gcctcgctga gccgagcaag ctcgagcgca
     3421 tcttcgatca gggttcgctg tccggactcg ctcatgtcgc tggtgtacgt cgaagccact
     3481 cgctggccga ccatcactct caaaccgagc gcccgcgacc cagcttcctc gaccaactcg
     3541 ggctcgccca tgcgagcctt gacggaaaga tgcgagccgc tgtgtacccc cgcctcggcg
     3601 acatctgcgc ctgcgcgcct tgcgcgttcg gtgaccgagc gccccagctc gatgagcgct
     3661 tctgcctctt gttgcgcgtc tctcatccgc ccgccacctg agtcccgccg acggtcatcg
     3721 aagcgatctt gatggtgggg cagccgacgc ctaccggtac cgactgtcct tccttaccac
     3781 aagtccagat cccatccgat acctcgaagt catgaccaag catgaccacg ttgcgcatcg
     3841 cctcgggccc gttgccgatg agattgacac ccttgagcgg cgcagtgagc ttcccatcct
     3901 cgatcaggta gccttcggtc aacgagaaga cgaagtcccc gttcgagata tcgacttgcc
     3961 cgccgccaaa tttcttcgcg tagacgcccc ggcggacact ccgaatgacg tcttccggtt
     4021 cgtcgacgcc gccgagaagc atcgtgttgg tcatgcgtgg catcggatgg gagcgaaagc
     4081 tctgccgacg accatgtccg tcggaggtcg ttccaaagaa gtcggcgctg tggcggtctt
     4141 gcatgtatcc cttcaaaacg ccgttttcga tgagaacgga acgacttggg agctgccctt
     4201 cgtcgtccac gttgatcgac cctcttgaac cttgcagcga agcgtcatcg acgaccgtgc
     4261 aaagcgcgct cgcgacttct tggccgatac ggtcagagta gttgctcgtc ttttttcgat
     4321 tgaaatccgc ttcgaggcca tgccccacgg cttcgtgcaa gaggatgcca ctgtcaccgg
     4381 gcgccaagac cacgtccatc tcgccggcag gtgcttcgcg ggcatcgagc atgaccaggg
     4441 cttgttggac tgcctgctcg gcgtgccact cgggcgtctt gtcttcgaaa tactcgaggc
     4501 cgatcctgcc gcccccgccg gaggaccccg actggcggtt tccgccgtcc tccgcaatca
     4561 cgtgaacgcc gaagcgaatc agtggttgcc ggtcatgggc catgtggccg aggctgttgg
     4621 cgatcaatat ctcgcgcatg ctctctgcga aagaagcatc gactcgaatg atgcgcggat
     4681 cggcggctct ggcagccttg tccgcgcgct cgagaatcgc cttcttggct tcaccctcca
     4741 catcgaagga ctcgacgtcg accggatagc gactgggggt cgagcgcatc gacacctctc
     4801 gaggggcgac cggctggccg ccggctgcga tctcagcagc ggtctcagcc gcctgcttca
     4861 tcgtatcgaa cgagaagtct tcgcagtacg cgtacccagt tgcgtctcct ttcatgacgc
     4921 gtacgccgag ccccatcgaa accgcccgcc gagcggactt caagatctgc tcgtcgtacc
     4981 caaagctgcc gctgaccgaa tactcgaaaa acaaatccgc gtagtcgccg cctttcgcaa
     5041 gagcgattct caggagacga ttcgcgatgt ccgcatcgac ggtgtgcgac ccctgcgcgg
     5101 cgaacggtgc gtgataacgg gctgccatga gctgccgcgg actctagagc gcgggcgcgc
     5161 cattggccac ttctatcggc gtaagaagcc aattacggct tcgatgatcc ggttctgacg
     5221 tgattcgccg aggcccgcga agatcggaag tgccaagacc tcacgactcg cctgttccga
     5281 gcgaggaagc gacccctctc gatagccgag gctcgcgaaa cattcctgac gatggagcgg
     5341 taccgggtaa taaatcgcgg tcgcgatgtt ctcctcctga aggtgcgcct gcagcgcgtc
     5401 ccgccgcgat gtacggatga cgtactgatt gtagatatga ccttcgaacc ggcgtgcagg
     5461 cgggcgaagc agcccctcgt ccaatccgga ttctgcaaac gcagcatcgt agtagttggc
     5521 gttttcctgg cggcgccgcg tccagccttc gagctcggga agcttcacgc gcagaagcgc
     5581 ggcttgcaga gcgtctattc ggaagttccc gccaatgacg gcgtgatagt acttgggctg
     5641 gctcccatgc gcacgcatga cacgagcctt ggaagcgagg ccggcgtcct gagtggtgac
     5701 caatcccgcg tcaccaaagc ctccgagatt cttcgacgga aagaacgaaa aacaaccgta
     5761 ggcgccg
//
LOCUS       DASRZF010000141         8310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_439401, whole genome shotgun sequence.
ACCESSION   DASRZF010000141 DASRZF010000000
VERSION     DASRZF010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 8310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8310
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439401"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="VFG22_06380"
     CDS             complement(<1..669)
                     /locus_tag="VFG22_06380"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HET6415903.1"
                     /translation="MRWRSSWLTLGLGAFLLVACNDGSDDLGSETDIALRIEISARGL
                     SGDPAVGLEIPSIEGPLAVLGRRLFFTQSLGGDLDAACVSCHHPMLGGGDGLAQSIGV
                     GADDPNVLGPGRTHPDGDLTVPRNAPTTFNLALWRKGMFWDSRVEELGQAGIRTPDSE
                     FGTSDPEAGDSISAAQARFPVTSSEEMLGFEFELGGTRDEIRAALEDRLRADGRWAAQ
                     FEAAF"
     gene            784..1224
                     /locus_tag="VFG22_06385"
     CDS             784..1224
                     /locus_tag="VFG22_06385"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET6415904.1"
                     /translation="MRTEQLFEETRLLFHALKQWAETLHEGLGVSTAMRGVLELLLRG
                     GPMTVPDMARARGVSRQHIQQQVDALLESELVERSANPAHRRSPMIALSDRGRALIQN
                     MQAREQNAISRLQVGVSDNAVEEAAQVLAAWRAGLQRDAQRRSA"
     gene            1325..2215
                     /locus_tag="VFG22_06390"
     CDS             1325..2215
                     /locus_tag="VFG22_06390"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HET6415905.1"
                     /translation="MGLIYYAIPFFLATLVLEHWLVSRKVSASEEEVKLAGFTTKDSF
                     ASLSMGIGFLLINALLTLLPFAGLAWLYQFRLFDIPVVWWSWILLLFAEDFCYYWFHR
                     LHHEVRALWAAHVNHHSSTHYNLTTALRQSWTTPFTGFIFWVPLPLLGFPIEMILIQK
                     SISLLYQYWLHTELIAKLGWFESVFNTPSHHRVHHGRNPVYLDRNHAGIFIIWDKMFG
                     TFEPEAQAVDYGLTKNIHTYNPVRIAFHEWYAMLRDAWNARTWRGRFGYLLMPPGWTE
                     DGHGKTSHALRVEYLAEKGV"
     gene            complement(2222..4975)
                     /locus_tag="VFG22_06395"
     CDS             complement(2222..4975)
                     /locus_tag="VFG22_06395"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HET6415906.1"
                     /translation="MQRLARFLVLVANVSLAGCGSDEVTPITGDLEWPPDATVYFDQY
                     GVFNADCATDEDCAMALGYYHASERFVQMDVRRRFSTGRLADILIPIIAQQYIDDFAS
                     IRASFSNREGRPLEEQLIEEASPKTLALLQAYSAGVNKWLEEVKAGDPNAIFPNEFSS
                     VFLDYNPEDVPAWTPQDCVASIIALIDSLTNDETEIMNAGKARAAINAREPDIGDDKF
                     ADLWDRGPLLESSILPPDWVPPSSSSAASKRLAMCGPNRPLDPGAALSRAADRLDAID
                     ALRKMVLGAGVLGEDVGSNNWAVSGSRTASGNALLANDPHLGMTQPATWYLAHMDGKT
                     NGGGEFHSAGVTFAGLPWILIGQNESIAWGMTTTNMDFTDVYVEEVVRDESGNATGVM
                     FEGNEVDFVRVPWTVTFSDGTTHDFCDGADCEGPPLLFVPHHGPLRDISLTEPPNPED
                     DVALTLRWTGQEISTDINFLTELNRATTVEEARMALELMTTVGQNVVVVDTAGSIGWF
                     PYNQLPKRTWATGLDGEAPPWLPLDGRSGDYEWDEYFELQELPQAMNPDKGYIATANN
                     DMTGALLDGDPTTLPSGAIHPPYQVSAASGYRFVRIVDLIEDIDDQHTIGTMNQIISD
                     VYSLIGEAMVPKMIEIAENPQTLPGINGLKVIEALKSWNFECPTGLVGPYWDSDLSDD
                     PDELLASAGCAAFHVLLDDLRARIEENEFAPSFYDGDARSPSAAMYFSIVDTSRLTPE
                     NKDIFWDNPATMEVETKYQVMGESLQVVGDFLDGWLGSDEKTQWAWGRLHGLRLLSDI
                     GGLLGARSYDNPASDDPLFANDGGLYTVDVAYPTPTTCLNDDVNCEPFVQTWGASTRF
                     VCEALPQGPSCTIQLPGGQSGHLQSDNYEDLIFPYLENQPIPLVFDIAEAEASAVRTV
                     VFQ"
     gene            complement(5470..6942)
                     /locus_tag="VFG22_06400"
     CDS             complement(5470..6942)
                     /locus_tag="VFG22_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HET6415907.1"
                     /translation="MGKVTGFKEFPRETIPYGDPLERISGWDEFTVDVPEEHLRKQGA
                     RCMDCGVPFCQSATGCPIDNLIPEWNDLVYRGRWREAVDRLHKTNNFPEFTGRVCPAP
                     CEGACVLGINEPAVTIKNIECAIVDKGFAEGWIKPEPPLQRTGKKVSVIGSGPAGLTA
                     AQQLNRAGHSVVVYERDDRIGGLLTYGIPNMKLDKDVVERRLEIMRAEGVEFVTNAHV
                     GVNKDAQKIRAESDALVLACGATRPRDLPIPGRDLKGVHFAMEFLLKNTKSLMDSKLN
                     DGEYISAKGKRVIVIGGGDTGTDCIGTSIRHGCTSLVNFELLPRPPAERAPDNPWPEW
                     PTIFRVDYGHQEAEAKFGSDPREYRVLSKRFIDDGKGNVAGVETVRVEWTKDDEGKWR
                     MSEVPGSEEIFEADLIFLAMGFLGPEDTLAEQLGLERDARSNFKAEYGKYASSTEGVF
                     VAGDCRRGQSLVVWGIAEGRGAARAVDTFLMGSSDLLAPD"
     gene            complement(6947..>8310)
                     /locus_tag="VFG22_06405"
     CDS             complement(6947..>8310)
                     /locus_tag="VFG22_06405"
                     /inference="COORDINATES: protein motif:HMM:NF013645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HET6415908.1"
                     /translation="GLPWELGIAETHQTLVMNDLRSRVVLQTDGQMKTGRDVVIGALL
                     GAEEIGMSTAPLIALGCIMMRKCHLNTCPVGIATQDPELRKKFKGKPEHVINYLFMVA
                     EEAREIMASLGFRTFEEMVGRVDMLDTVDAIVHWKAQGIDLSPILTKAKKKNAVTDVY
                     CTKQQDHGLDKALDNRLIELAQPAICEGKRVRASLTIKNTNRTVGTMLSHEIAKKWGE
                     ELLPDGTVQFKFDGSAGQSFGAWLAKGVTLELEGDANDYVGKGLSGGTIIVYPSKKAT
                     FVPAENVIVGNVVLYGAVSGKAFFRGRAAERFCVRNSGAWAVIEGVGDHACEYMTGGR
                     VVVLGETGRNFGAGMSGGVAYVLDRNGVFHSRCNQEMVELERLQYPEEIAILKGLIQE
                     HQDRTRSKVAERLLQDWEQALKTFVKVMPTDYKRVLRQTGDLSVGVPGPDAAELQDLP
                     AAE"
BASE COUNT         1565 a         2569 c         2482 g         1694 t
ORIGIN      
        1 aaatgccgct tcgaattgag cggcccagcg gccgtctgcg cggaggcgat cctcgagcgc
       61 ggcgcgaatc tcgtcgcgag tgccgcctaa ctcgaactcg aagcccagca tctcttcgga
      121 cgaggtgact gggaaacggg cctgcgcagc ggagatgctg tcgcccgctt ccggatcgct
      181 ggtgccgaac tcgctgtccg gcgtccgaat accagcttgc ccgagctcct cgactcgcga
      241 atcccagaac attcccttcc gccaaagggc gagattgaag gtggtcggcg cattgcgagg
      301 cacggtgagg tcgccgtcgg ggtgggttct acctgggcca aggacgtttg gatcgtccgc
      361 gccaacgccg atcgattggg cgagtccgtc cccgccgcct agcatcgggt ggtggcagga
      421 gacgcaagca gcgtcgagat cgcctccgag gctctgcgtg aaaaagagcc tgcgcccgag
      481 gacggctagc gggccctcga tgctcgggat ctccagaccc accgcggggt cgccgctcaa
      541 gccgcgcgcg ctgatctcga tccgtagcgc gatgtcggtt tcgctgccga gatcgtccga
      601 tccgtcattg catgcaacga gcaggaaggc gccaaggccc agggtgagcc agctgcttct
      661 ccacctcata gatgaccgcc aaagcaaggt aaggtctctt cttaccacac agagcctcgg
      721 ggcgtcatgg gcaggctcgt ctcaacttga caaaactccg tcgttatgac aagtatcttg
      781 tcaatgcgaa ccgagcagct atttgaggaa acccggctcc tgtttcacgc gctgaaacag
      841 tgggcggaga ctttgcacga gggactgggc gtgagcactg cgatgcgagg tgtgctcgag
      901 ttgctccttc gaggcggccc catgactgtg ccagacatgg cgcgcgcccg cggcgtgtcg
      961 cgccagcaca tacagcagca ggtcgacgcg ttgctcgaaa gcgagctcgt cgagcgctcg
     1021 gccaaccccg cacatcggcg ctccccaatg atcgcgctga gcgatcgggg tcgggcgctc
     1081 atccagaaca tgcaggccag ggagcagaac gcaatctcgc gtcttcaggt aggcgtctcc
     1141 gacaacgccg tcgaggaagc cgcgcaggtg ctcgcggctt ggcgcgccgg ccttcagcgc
     1201 gacgcgcaga ggaggtcggc atgagagaac gaatcattca gcagttcaaa cgcccagaag
     1261 gcatcctcgg acaggcttag tcgtgctgct tgtgggactc acgacttgat cgtaattctt
     1321 cggcatgggt ctgatctact acgcgatccc cttctttctc gcgaccttgg tgctcgagca
     1381 ctggctcgtg agtcgaaaag tgagcgcttc ggaggaagaa gtgaagcttg ccgggttcac
     1441 caccaaggac tcgttcgcaa gcctctcgat gggcataggc ttcttgttga tcaacgccct
     1501 gctcacgctc ctcccctttg ccggcttggc gtggctctat cagttcaggc tcttcgacat
     1561 acctgtcgtc tggtggtcct ggatcctcct gctcttcgcg gaggactttt gctactactg
     1621 gtttcaccgg cttcatcacg aggtgcgggc actgtgggcc gcacatgtga accaccattc
     1681 gagcacccac tacaacctca cgacggcact gcggcagtcg tggacgacgc cattcacggg
     1741 tttcatcttc tgggttccgc tgcctcttct aggctttccg atcgagatga tcctgatcca
     1801 gaagtcgatc agtctgctct accagtattg gctacacacc gagctcatcg caaagctcgg
     1861 ctggtttgaa tccgtcttca acacgccctc gcaccacagg gttcatcacg gacgcaatcc
     1921 cgtttacctc gaccgcaacc acgccggcat cttcatcatc tgggacaaga tgtttggcac
     1981 cttcgagccc gaggcccagg cggtcgacta cggcctcacg aagaacatcc acacctacaa
     2041 tccggtgcga atcgcctttc acgagtggta tgcgatgctc cgcgacgcgt ggaacgcgag
     2101 aacatggcgc ggccgcttcg gttacctgct gatgcctcca ggttggacgg aggacgggca
     2161 cggcaagacg tcacacgcct tgcgagtcga gtacctggcc gaaaaagggg tctgaccgcg
     2221 tctattggaa gacgacggtg cggaccgcgc tcgcctcggc ttccgcgatg tcgaaaacca
     2281 gcggtatggg ttggttctcg agataaggga agatcaagtc ttcgtagttg tcagactgaa
     2341 gatggcctga ctggccgccg ggaagctgga tggtgcaaga cgggccctgc ggcagagcct
     2401 cgcacacgaa gcgggtcgag gcgccccagg tctgaacgaa cggctcgcag ttgacatcgt
     2461 cattcagaca tgtggttgga gtgggatagg cgacatccac ggtgtacagg cccccatcgt
     2521 tggcgaagag cggatcgtca gatgccggat tgtcgtagct ccgggcgccg agcagccctc
     2581 cgatgtcgga aaggagacgt agcccgtgca ggcgccccca tgcccactgc gttttttcgt
     2641 cgctaccgag ccagccgtcc aagaaatcgc cgaccacttg gagcgactcc cccatcacct
     2701 gatacttggt ttcgacctcc atcgtagcgg ggttgtccca gaagatgtcc ttgttctcgg
     2761 gcgtgagcct gctggtgtcg acgatggaga agtacatcgc agcgctcggc gaacgcgcat
     2821 ctccgtcgta gaagctcggc gcgaactcgt tctcttcgat gcgggcccgg agatcatcga
     2881 gcagcacgtg gaaggccgca caaccggcag atgccagcag ctcgtcggga tcgtctgaca
     2941 agtccgagtc ccagtacggg cccacgagcc ctgtcggaca ctcgaagttc caagacttga
     3001 gcgcttcgat cactttgagc ccgttgatgc cgggcaacgt ctgcgggttc tctgcgatct
     3061 caatcatctt cggaaccatg gcttcgccga tgagcgagta gacgtcgctg atgatctggt
     3121 tcatcgtccc gatcgtgtgc tggtcgtcga tgtcttcgat caggtcgacg attcgcacga
     3181 atcggtatcc cgaggccgcc gacacctgat agggaggatg aattgcacca ctcgggagcg
     3241 tcgtgggatc gccgtccagg agcgccccag tcatgtcgtt gttcgcggtc gcgatgtatc
     3301 ccttgtcggg attcatggcc tgtgggagct cttgaagctc gaagtactcg tcccactcgt
     3361 agtcgccact gcgcccgtcg agcggaagcc agggcggcgc ctcgccatcg agcccggtgg
     3421 cccacgtccg cttgggaagt tggttgtagg ggaaccaccc aatgcttccg gcggtgtcga
     3481 caacgactac gttctggccg acagtcgtca tgagttctaa cgccatgcgc gcttcctcta
     3541 ccgtggtggc gcggttgagc tccgtgagga agttgatgtc ggtgctgatc tcctggcccg
     3601 tccagcgaag agtgagtgcg acgtcatcct ccggattcgg gggctccgtc aatgagatgt
     3661 cgcgaagcgg cccgtggtgc ggaacgaaga gcagaggcgg tccctcgcag tcggcgccgt
     3721 cgcagaagtc gtgagtcgtg ccgtcgctga acgtgaccgt ccaaggaacc ctcacgaaat
     3781 cgacctcgtt gccttcgaac atgacgcccg tcgcattgcc gctttcgtcg cggactacct
     3841 cctcgacgta gacatcggtg aagtccatgt tcgtggtggt catcccccat gcgatgcttt
     3901 cgttttggcc gatcagaatc cagggcaggc ccgcgaacgt cactcccgcg ctgtgaaatt
     3961 ccccgcctcc attggtcttg ccgtccatgt gagcgaggta ccaagtcgcc ggttgggtca
     4021 ttccgaggtg agggtcgttc gccagcagcg cattgccact agcggtgcgt gagccagaca
     4081 cggcccagtt gtttgatccg acgtcttcgc caagcacgcc ggcgcccaaa accatcttgc
     4141 gaagtgcgtc gatggcgtcc aacctgtcgg cggctcgact gagcgcggcg cctgggtcca
     4201 atggacggtt cggaccacac atcgcgaggc gcttcgacgc ggcggaagat gagctcgggg
     4261 gaacccagtc gggcggcagg atggacgact ccagtagtgg gcctcgatcc caaaggtctg
     4321 cgaacttgtc gtcgccgata tcgggctcac gcgcgttgat agccgcacga gccttacccg
     4381 cgttcatgat ttcggtttcg tcgttcgtta gactgtcgat cagcgcgatg atcgaggcga
     4441 cgcagtcttg aggcgtccac gcagggacat cttctgggtt gtagtcgagg aagacgctcg
     4501 agaattcgtt tgggaatatc gcgttggggt cgccagcctt cacttcctcc agccacttgt
     4561 tgacgccggc actgtaggct tggagtagcg cgagggtctt tggagaagcc tcttcgatca
     4621 gctgctcttc gagaggcctg ccttctcggt tcgagaaact agcgcggatg gacgcgaagt
     4681 cgtcgatata ctgctgagcg ataatcggaa tcaagatgtc agccagtcga ccggtcgaga
     4741 acctccgccg aacgtccatc tgcacgaacc gctctgacgc gtggtagtag ccgagggcca
     4801 tcgcgcaatc ctcgtcggtt gcgcaatcgg cgttgaatac gccgtactgg tcgaagtaga
     4861 ccgtcgcgtc gggcggccat tcgagatcgc cagtgatggg agtaacctcg tcgctgccgc
     4921 agcctgccag ggaaacatta gcgacgagaa cgaggaatcg agcgagtcgt tgcatcgggg
     4981 accacctctt tgggataaga atgggctaga acataatccg tacacgtcaa aatccaaatg
     5041 acgctcctgg ggccccgtgc gcctgcgatc gactcaaccg atggatggga gtcgaatggg
     5101 cagcgtgggg ctggttgcaa gtaagggcgc cgggtgaatc gactgatcca cccggcgttg
     5161 agcaagcaag cttccgccgc tagttgcgcc gacagcggcg aatccccaag ccaaagacaa
     5221 gcgtcacgag aagaagaaca gcggtggacc cgtcatcgcg gatcacccgg acgtgacagc
     5281 caacgccacg cccacctaaa tttggctaag cctccatctt ggaccacgtg ggtcatccct
     5341 ttctacctgc ggcccttgag cccccggtag aaacgattac acaatatcaa cggattcgtg
     5401 cagtttcaac ggatgcgcta aaaaacggtt cctcggctgc tgggagacga taaacccttt
     5461 gatttcagcc tagtccggcg ctaacagatc ggagcttccc atcaggaagg tgtcgaccgc
     5521 gcgcgctgcg ccccgccctt cggcgatacc ccagaccacc aaggactgcc cgcgacggca
     5581 atcgccggcg acgaagaccc cttcggtgct gctcgcgtac ttgccgtact cggctttgaa
     5641 gttactgcgc gcatcgcgct cgagaccgag ctgttcggcc agcgtgtctt cgggaccgag
     5701 gaagcccatg gcgaggaaga tcaaatcggc ttcgaagatc tcctcggagc cagggacttc
     5761 gctcatccgc cacttgccct cgtcgtcctt ggtccattcg acgcgaaccg tctcgactcc
     5821 ggcaacgttc cccttgccgt catcgatgaa gcgcttgctg agaacacggt actcgcgagg
     5881 atcacttccg aacttcgctt cggcctcttg gtgaccgtaa tcgactcgga agatcgtagg
     5941 ccactccggc cagggattgt ccggcgcgcg ttcggcgggt ggcctcggca agagctcgaa
     6001 gttcacgagt gaggtgcagc catggcgaat cgaagtgccg atacaatcgg tacctgtatc
     6061 accacctccg atgacgatga cgcgcttgcc cttggcgctg atgtactcgc cgtcattcaa
     6121 cttgctgtcc atcaagctct tggtgttctt gagcaagaac tccatcgcaa agtgaactcc
     6181 tttgagatcg cggcccggaa tcggcaaatc gcgcggacgc gtcgcgccgc aggcgagcac
     6241 tagagcatcg ctctccgctc gaattttctg ggcgtccttg ttgacgccga cgtgagcgtt
     6301 cgtaacgaac tccacgcctt ccgcacgcat gatctcgagg cgccgttcga cgacatcctt
     6361 gtcgagcttc atgttgggaa tcccgtaggt caggagtccg ccgattcggt cgtctcgctc
     6421 atagacgacg acgctgtgac ccgcgcgatt gagttgctga gccgcggtga gccccgccgg
     6481 accgctcccg atgactgaga ctttctttcc ggtccgttgc agcggaggct cgggtttgat
     6541 ccagccttcg gcgaaaccct tgtcgacgat cgcgcactcg atgttcttga tcgtgacggc
     6601 gggctcgttg atgccgagca cacaggcgcc ttcacagggc gcaggacaga cgcgtccggt
     6661 gaactcgggg aagttgttgg tcttgtgcag gcgatcgacg gcttctcgcc agcgtcccct
     6721 gtagaccagg tcgttccatt cggggatcag attgtcgatc gggcagccag tcgccgattg
     6781 gcagaacggc acgccgcaat ccatgcaacg cgcgccctgc ttccgcaggt gctcctccgg
     6841 aacgtcgacc gtgaactcgt cccagccgga gatgcgctcc aatgggtcac cgtacggaat
     6901 cgtttctctc ggaaactctt tgaaaccagt taccttaccc atcgtcctac tccgccgccg
     6961 gaaggtcctg gagctcggcg gcatcgggcc cgggaacgcc caccgaaagg tcacccgtct
     7021 gacgaagcac acgcttgtag tcagtcggca tcaccttgac gaacgtcttc aatgcctgct
     7081 cccagtcctg cagcaatcgc tcagcgacct tcgatctggt gcgatcttgg tgctcttgaa
     7141 tgagaccctt gagaatcgcg atctcttcag ggtactggag ccgctcgagc tcgaccatct
     7201 cctggttgca cctgctgtga aagacgccgt ttcggtcgag gacatatgcg acgcccccgc
     7261 tcatgccggc cccgaagttt cgcccggtct cgccgaggac tacgactcga ccgcccgtca
     7321 tgtactcgca ggcatgatcg ccaacgcctt cgatgacggc ccatgcaccg gagttccgaa
     7381 cgcagaatcg ctccgccgcg cgtccacgaa aaaacgcctt tccactgacc gcaccataga
     7441 ggacgacatt gccgactatc acgttctccg cagggacgaa cgttgccttc ttcgacggat
     7501 aaacgatgat cgtgccgcct gagagcccct tgccgacgta gtcgttcgca tcgccttcga
     7561 gctcgagtgt gacgcccttc gcaagccatg cgccgaagct ctgacccgcg gagccatcga
     7621 atttgaactg caccgtgcca tcgggaagaa gctcttcacc ccatttcttg gcgatctcgt
     7681 ggctcagcat cgtgcccacc gttcgattgg tgttcttgat cgtgagcgac gcgcgaaccc
     7741 gcttgccttc gcaaatcgct ggctgcgcga gctcgatgag cctgttgtcg agcgccttgt
     7801 cgaggccgtg gtcttgctgc ttcgtgcaat aaacgtcggt aacggcgttc ttcttctttg
     7861 ccttcgtcag gattggcgaa agatcgatgc cctgtgcctt ccagtggacg atcgcatcga
     7921 cggtgtcgag catgtcgaca cggccgacca tctcctcgaa ggtccggaac ccgagcgagg
     7981 ccatgatctc gcgcgcctcc tcggcgacca tgaagaggta gttgatcacg tgctcgggct
     8041 tacccttgaa cttcttgcga agctccggat cctgtgtggc gatgccgacc ggacaggtgt
     8101 tgagatggca cttcctcatc atgatgcagc cgagcgcgat gaggggcgcg gtgctcatgc
     8161 ctatttcctc agcgccgagc agggcgccga tgaccacgtc tcgcccggtc ttcatctgtc
     8221 cgtcggtttg aaggaccact cggcttctca gatcgttcat gaccagagtt tggtgcgtct
     8281 cagcaatacc aagctcccat ggaagccctg
//
LOCUS       DASRZF010000142        25677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3533713, whole genome shotgun sequence.
ACCESSION   DASRZF010000142 DASRZF010000000
VERSION     DASRZF010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 25677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25677
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3533713"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="VFG22_06410"
     CDS             <1..892
                     /locus_tag="VFG22_06410"
                     /inference="COORDINATES: protein motif:HMM:NF016611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6415909.1"
                     /translation="MALRGHLTCRPCYPADVHWIGVVLACTLWLTATTSFADAANAHY
                     QLAKTLRAEGRYEEALAEVDLAVAERPTYAQGHLTRGSILRRLGRYQEALQAFERAVQ
                     LEPEDGGAQGLTGAALIRLERPKEAVPYLKRASELEPKNSNHWLNLGVAYRKSGDNDA
                     AIATYEKGLEAIPNDPQILNNLGVALRKARRYDEAIKALEKASAAAPHDAEVARNLAI
                     AYRGAKHYGQAVPIYIRALELGRGGPPDLLFDLASCYEKLGNDEMAIATFERYIAATN
                     ESDPAGANLARRAVENLKSK"
     gene            complement(929..1465)
                     /locus_tag="VFG22_06415"
     CDS             complement(929..1465)
                     /locus_tag="VFG22_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415910.1"
                     /translation="MKRFASAPVLLSLLLLGSWGTFGPVPTASADSDLVVIDGVFGGT
                     VSKYSGQRKWQPSLWLDATYRVAGPLHLGGYFQWLGKDWPLDEPGLGGGGVIVLRTNI
                     KKLRLSGAFGGGYLRVPLPDSVEGSGTISAFVGLGYGFLSWMGLEVRGRWMRYFKMPT
                     DAPDQAWTIEAGFSFYID"
     gene            complement(1526..3286)
                     /locus_tag="VFG22_06420"
     CDS             complement(1526..3286)
                     /locus_tag="VFG22_06420"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF013461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HET6415911.1"
                     /translation="MPTADPRPFEVEYIENPTQAELRELALAHTPCVQKTSVGSINKV
                     TRNKARMAKYTYIIDTQDRWSHQIIDPAKARGLIERQAKYIADKGKLIAIDGYSGLGR
                     GAFSTTWLYTLEGANIAGMQQVLAFPRSAVESKAKLAEPFEPTFRLFYTPDLMLDDMP
                     GRQAIIVDMENWTTYVMGADYFGESKKGILRMLNHYVYEQGGLVLHAGAKAVTPKSGN
                     KLTMTIMGLSGTGKTTTTFSKQGDLTEPIQDDMVSLWPHGAISITENGCFAKTDGLSL
                     ETEPVLYKSTIGPDAWVENVFLEADGSFNFSKGVLSIEDVKRLRDILIATGADAGKVD
                     KYIAGGVNIDEIVDSHGVPADGWDFVVWTQNGRSIVPLSSIPDAADLENIPEVKSMGI
                     LNRDEGYDAAMPGIVRFTSPEQAAGYFMLGETSKTSAAGKERGKTRSPFTQPFFPAKH
                     GLQAKRFAELAATMPGVSMWLMNTGYVGGEANDAKENKALNIKIPHSSAMLEALLSDA
                     VKWKTDPDFGYEIVDVDAPENAELVSKVPAEILEPRRFFDSRGRHADYEQWVRHMKEA
                     RTEFLKKFDVSDVIIRATAG"
     gene            3427..4554
                     /locus_tag="VFG22_06425"
     CDS             3427..4554
                     /locus_tag="VFG22_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HET6415912.1"
                     /translation="MRALPLNFRDAEGFFDPDWSDWRWQARNSVTNLQGLDKALDLTD
                     AERIGAARAMAAGLPISITPYYLALCDPKDPDCPIRRQCVPRAEEAIAVKGDLRDPLG
                     EEAHQVAPHLIQRYPDRVLLLATDRCGVYCRFCTRSRLVGDGGGARSMTALEPAFAWI
                     EAHPEVRDVIISGGDPCVMSTERLSRLLLRIREIDHVDYVRLATRAPVTLPQRITEEL
                     AAAIRESHESTWIMTHFNHPKELSEEARAACARLVDAGLPVMNQTVLLRGVNDDQNTL
                     EELFRGLVRSRVRPYYLLQMDPVGGTGHLRTPLRRGIELMAALQGRMSGIALPKLIVD
                     TPGGLGKVPIGPNYLRSEQDGVTVLETFRGDLVEYYDPLEL"
     gene            4646..5293
                     /locus_tag="VFG22_06430"
     CDS             4646..5293
                     /locus_tag="VFG22_06430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HET6415913.1"
                     /translation="MQRALTLHQTTIGKKVIMALSGLIIVGFAIGHFLGNLNIYLGQE
                     ALDGYAATLQSLPPLIWGTRLLLLFAFGAHIASAFSLWVRNMKARGSRYKKHQDLATD
                     YAARTMYWSGPILLLFVVYHLLHFTILPAHPGEVYRNVVEGFQVPLIAGVYMVGNIAL
                     GLHIYHGIFSALQTLGASHPRYNSYRRDAAIAISAAITIGNLSFPIAVLAGFVTL"
     gene            5302..7233
                     /locus_tag="VFG22_06435"
     CDS             5302..7233
                     /locus_tag="VFG22_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HET6415914.1"
                     /translation="MELRSNEPTGPIENRWAQHKENSKLVAPANRRKYSIIVVGSGLA
                     GGAAAASLGELGYNVKCFCYQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRLFYDTVK
                     GGDFRSRESNVYRLAELSVQIIDQAVAQGVPFAREYGGLLGNRSFGGAQVSRTFYARG
                     QTGQQLLLGAYQALSRQIEAGTVEMFPRTEMLDLIVHDGRARGIVTRDMTTGEVSPHL
                     ADMVILCTGGYGNVFYLSTNAKGCNCMAIWRAHRHGAAFANPCYTQIHPTCIPQAGTY
                     QSKLTLMSESLRNDGRVWVPRKKEDVTKRPEDIPEADRYYYLEERYPAFGNLVPRDVA
                     SRNALYVTNDGLGVGPEVDGERRGVYLDFADAIKRLGEQAIRERYGNLFDMYERIAGE
                     SPYKTPMRIYPATHYTMGGLWVDYNLETTIPGLFSGGEANFSDHGANRLGASALMQGL
                     ADGYFVLPTTVGNYLGEVRTLAPLTEDHDDVTQAVKAVEERIHKLMNAPDPKHAPEHF
                     HRKLGRIIWTKCGMSRNREGLEEALREIPKLRDQFWREVKITGTGTELNQTLERAGRV
                     ADFLELGELMCIDALKREESCGGHFREEHEENGEALRDDENFTYVAAWEWTGNPGQPR
                     LNKEPLSFEYVKPSKRSYK"
     gene            7245..8030
                     /locus_tag="VFG22_06440"
     CDS             7245..8030
                     /locus_tag="VFG22_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HET6415915.1"
                     /translation="MSDELLNLTLHVWRQDGPNEPGRFEAYQAKDITTHMSFLEMLDV
                     VNEELIEEGKSLIAFDHDCREGICGSCGMVVNGMAHGPQRMTTTCQVHMRQFKNGDEI
                     WLEPWRAKGFPIIKDLIVDRSAFDRIIQAGGYISIRTGSAPEANEMPIAKEIADQAMD
                     AAECIGCGACVAACPNASASLFTAAKISHLSLLPQGQAERQDRARKMVAQMDAEGFGG
                     CTNHGECEAACPKGVSLRWIARMNRELVRSMVKADTPIASRER"
     gene            complement(8027..8638)
                     /locus_tag="VFG22_06445"
     CDS             complement(8027..8638)
                     /locus_tag="VFG22_06445"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HET6415916.1"
                     /translation="MLDEFLHTLEHGPLYWSYLLVAASAAIEYVFPPIPGDTVALFAV
                     ALAARAQLNWVFVYVSMTLGAMAGGLAAWGFGAWLARHEESWPSFLKSPKAVRALDAV
                     RRGYERHGAMYLAANRFLPALRAFFFVGAGLSRMSLGAVILYGGISAALWNAMLLGAG
                     YAVGSNWEALSDLAERYTIATLVFVILAIVAVAARFVWDDRRS"
     gene            8761..9558
                     /locus_tag="VFG22_06450"
     CDS             8761..9558
                     /locus_tag="VFG22_06450"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HET6415917.1"
                     /translation="MSVLQRPNARVEDVAILIAKEANPVLDEHAMRRSLDGLGAEVLI
                     RRGLRSTPERDGRILAEFLFQELGFEGELDDYYNPYNSYLDKVMLRRKGIPISLSVLT
                     MVVGERAGMDVEGVGFPGHFLVRVGGPNGVYQDPFHGGELLDGNGLRRLAAQFLGVEM
                     ALHPSYLEPVDCHTITIRMLANLKNAHRRQGDYARAMLACDHLVELTQAPEHRRDRGL
                     LAHALRTFGAASEDLAAYLEARPDAKDAVAISLALEEARQQADQFLH"
     gene            9622..10716
                     /gene="hisC"
                     /locus_tag="VFG22_06455"
     CDS             9622..10716
                     /gene="hisC"
                     /locus_tag="VFG22_06455"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HET6415918.1"
                     /translation="MSIESLKALFRPELAELTAYRVPPAAPAVKLDANESPFELPPEA
                     RARIATMLHATEMNRYPDGRATALRNALAHGLGGSADEYVLGVGSDELIALLATAMST
                     PRAGAQRPVVAFPDPTFVMYAMTSRAHGWEPVGVPLDDDWDLDTNAMARALERRNPKI
                     AYYATPNNPTGNCFSPEKIELLVDAFPNTLHVIDEAYGAYSEQSFATFAEERAHCALM
                     GTLSKVGFAGIRVGWLRIHEALSAELEKVRQPFNLNTASQVIATLALTDLAAVIEEHV
                     TLVVAERERLAKQIDAYQELHCFPSDANFLLVRYTGDVRELSAALLDREIAVRLFLHG
                     DLRLQSCLRITIGTPDENQRLLEALSDILG"
     gene            10720..11646
                     /locus_tag="VFG22_06460"
     CDS             10720..11646
                     /locus_tag="VFG22_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HET6415919.1"
                     /translation="MSVAREITPERAVEALNWAANVAEDPTLLEPQPERPTPAEWRSV
                     PVSDFRAQMDRLLTAKHPEPGLDAIESLGCLDVWLPEVAAMVGFGDNEWRHKDVWKHT
                     KQVVKQSVPRPEVRWGALLHDIGKPKTRRIDERGNVTFHGHAEVGASMFRKRVAERIG
                     FEGAQRERVHFLILHHLRAAQYDEDWTDAAVRRFYKQMGDGLRDLLDLSRADITTKRP
                     EKRRRGVRQISLLAKRIRDLQEEDSKVAPLPKGLGAVIMSEFGVPPSKRLGDLMKQLT
                     AAAEAGEVEREQSAEYYVEYLSAHRAEFGLND"
     gene            complement(11669..12793)
                     /locus_tag="VFG22_06465"
     CDS             complement(11669..12793)
                     /locus_tag="VFG22_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415920.1"
                     /translation="MPRPRHHIVLVPGLFGFAKLGGFDYFMHVEEALASRFFERGADC
                     EFVLVSSPPTGSIVYRTEVLLDAIERNCGGDSGAIHLVGHSTGGLDVRLLASPSGSPE
                     APVWLDRVRTVTSIATPHYGTPLAYFLTTLAGTQLLEALSLLTYFTLRAGGPPLTVLA
                     PLIASLGHMDRALGLEIKLLERATELLLKFLGNEGQAEVREWIEGIRGDQGAIIQLTP
                     ESMDIFNAGIEDNANIRYGCVVTRMPAPAPVRLIRDLRSPASLLSALIFSTLYVGAGR
                     TSKVYPPPNPAPEVREYLETALGRTLDNSLNDGLVPTGSMIWGDVLWGGTADHLDILG
                     HFHGDRDSAHTDWMVSGAGFREDDFDEVMDAVAEFQLRRP"
     gene            complement(12853..13251)
                     /locus_tag="VFG22_06470"
     CDS             complement(12853..13251)
                     /locus_tag="VFG22_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415921.1"
                     /translation="MVKKSVQVGLDARRRCPCFGMFCFLCAFITSIFGAKSASAESPL
                     PAIRTTQIALPISVLGLNNPYLIAAATRTPRVIRFLLSRDIRDLAARVDKLARYGVDL
                     NQRKLPHTEKLQLRVRQRGPGGVVLLSYRH"
     gene            complement(13499..14716)
                     /gene="ftsZ"
                     /locus_tag="VFG22_06475"
     CDS             complement(13499..14716)
                     /gene="ftsZ"
                     /locus_tag="VFG22_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HET6415922.1"
                     /translation="MAISIEFADDAELQARIKVVGVGGSGGNALNTMIQRGLEGVEFI
                     AANTDAQALDRSLAQVKIQLGPELTRGLGAGGNPDVGRKAALEDVERLSEALEGADMV
                     FITAGMGGGTGTGAAPIISQVAHDQGALTVGVVTKPFLFEGRKRSKNAERGVQELIDV
                     IDSIITIPNQRLMSLGDDDITMLDAFARADDVLLQAVQGISDLIINAGMINVDFADVK
                     TIMSNNGRALMGTGIAKGDRRALEAAEMAVNSPLLDEVSVQGATGILINFTAGPDIKL
                     REINEAAGLVQQAAHEDAEIIFGVVTDPNLTDTVKVTVIATGFEKSGHAPMPIGLRHA
                     HSSNQSSNGSSANRAMRTVAARGDDLSSTLEGVPAVVPQMAGARAFGASALHDEAVLD
                     IPAYLRRGNRTVE"
     gene            complement(14726..15955)
                     /gene="ftsA"
                     /locus_tag="VFG22_06480"
     CDS             complement(14726..15955)
                     /gene="ftsA"
                     /locus_tag="VFG22_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HET6415923.1"
                     /translation="MAENEIIAGLDLGSTKVCAIVAEVSEEGLDIIGIGSVPSKGLKK
                     GVVVNIESTVQAIRAAVEQAETMAGVEINAVYAGIAGSHVRGMNQDGVAAIANREVSA
                     EDVMRVLEQAKAVPLPGDRQVLHVLPQEYIVDEQDGIREPIGMAGVRLEARVHMVTAA
                     STSVANVTKCAERCGLFVEEIVLQPLASAHAVLSSDEREIGGVLIDIGGGTSDIIIYV
                     DGAVVHTSSIPIGGINLTSDIATGLRTPMAEAERIKIKYGCAASRMVDDDETIEVPSV
                     GGRAPRSLPRRVLCDIIEPRVEEIFAACRHVIAETGYMDMLASGAIVTGGTTLLDGLP
                     ELAEEVLGLPVRRGAPTGIGGLIDVVKSPSYATGVGLVKHGAEQLAGPSAPDRTVEVA
                     ASRGLGRKIGAWFKEVF"
     gene            complement(16088..17173)
                     /locus_tag="VFG22_06485"
     CDS             complement(16088..17173)
                     /locus_tag="VFG22_06485"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HET6415924.1"
                     /translation="MNRRRSPSSARKKPAPKKPLQGSPAESKTSPSRPVRKKNSAAKK
                     TSSTATTRKTALKATPRKKIPRKTAKSDRGQNRRRPVAKKPRRSKSTKSWRERFAGAC
                     EGVRAWVISHGSLFRRTGQLILLGAGAAALLWLGDRTVQYAHSAEAFEIREIIIEGNH
                     QLGEIDVRRAARLQIGSNIFEVSSEGTRNHLLQHPWIEEANVVRRLPGRVRIEIVERK
                     PVALVALDQLYLVSEEGAVFKRLGVDDPVDLPVITGIASERFYDDLDYRTAILLRSMA
                     LLQDYEGAGLAEREPVSEIHFEGAHGIELFVGDDGMNVRLGNGQHRQKLRRLRQVLER
                     LEREKTRPSYVYLDNVRSPERVTVRLP"
     gene            complement(17170..18543)
                     /gene="murC"
                     /locus_tag="VFG22_06490"
     CDS             complement(17170..18543)
                     /gene="murC"
                     /locus_tag="VFG22_06490"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HET6415925.1"
                     /translation="MFRGRIRSIHFVGVGGVGMSGIAEVLLDSGFEVTGSDLRENEYT
                     KRLAEKGAKIFVGHQAEQVKHADVVVFSSAVPPTNPELLEARRAGVPVIPRAEMLGEL
                     MRLKDGIAIAGSHGKTTTTSLVATVLRGAGLDPTVIIGGKLNALGSGAAMGAGDLLVA
                     EADESDGSFHHLIPSIAVITNIDPEHLDHYGSLDNVKEAFVGFANRVPFYGLVVACLD
                     HPNVQDVLPRLDKRIVTYGFSAHADYRARNPVFSGLSVGFDLEHHGKSLGQFEVRMPG
                     IHNVLNALATIAVADELRVDHDQVRSALRDFEGVQRRFSIVGEAAGVTVIDDYGHHPA
                     EVEVTLEAAQQAYGSRIVVAFQPHRYTRTRDLFDELTRAFNRADVLFLTEVYPAGEEP
                     IDGADARRLAEAIRAHGHRDVTLVEDRKDLAEAMATRIQPGDVVITLGAGDITRTGPE
                     LLEVLRR"
     gene            complement(18550..19824)
                     /gene="murG"
                     /locus_tag="VFG22_06495"
     CDS             complement(18550..19824)
                     /gene="murG"
                     /locus_tag="VFG22_06495"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HET6415926.1"
                     /translation="MIQRIMVAGGGTGGHLFPGLAVVDELRRRLPEIEVRFVGTARGI
                     ESRILPRRGESLELLEVTPLKGQGLGARFKSFARIPTAMKKASALMDDFEPDLVLGVG
                     GYASGPVLLSASLSGRPTALLEQNAQVGMTNRILARFVDRAYISFEQTEEVFGRRKSR
                     LTGNPVRQEFVEHARLALADPEGFESRARTILVLGGSQGARKLNEDVPRALAKAGIAA
                     RRLEVVHQTGESMRDQVEAAYQELGIQARVVTFIDEIARAYSNAALVIARAGATTLAE
                     LCAIGRPSILVPFPFAADDHQTKNAQALEEQHASICLREADLEVDALGATIADLLDDP
                     VRRQAMAQAARDHGRPDAAAAIVDDMMAWVGGADPLGAEAAQPEREPPAGLQYSGLAM
                     APILRLGCPEVALRPASHRPRRPVVVDGALWE"
     gene            complement(19821..21020)
                     /gene="ftsW"
                     /locus_tag="VFG22_06500"
     CDS             complement(19821..21020)
                     /gene="ftsW"
                     /locus_tag="VFG22_06500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HET6415927.1"
                     /translation="MTRSSNPAPLAEPGPADVTLAATLVGLIAFGVVMVYSASAVYAN
                     NMFGNGYHFLVRQTVFAALSFVVLIIFTRVNLGLLRRSTYPVLLLAILLMIAVALGFG
                     RSAGGATRWLALGPVNVQPAELAKLAMIMWLAHSLTKKSNRVRTFSIGFLPHVLVAGL
                     LMLLCLAQPDFGSAVMIALLTFVVLFAAGAKAGYLLGCVLLALPVGYAAIASSPYRMR
                     RIKAFLEPFEHRQGAGYQITESLMSFGSGGWTGVGLGDGRQKLLFLPEAHTDFISAIV
                     GEELGFIGVTLLCAAFAWLVFRGLRAAWRAQDEYAGYLAAGMTLFIGLQAFTNLAVAM
                     GLLPTKGLALPFVSYGGSSLIVNAAAAGMILNTSRFGRSRDEESPAQAKVKQAQPRLR
                     TASGGAR"
     gene            complement(21017..22375)
                     /gene="murD"
                     /locus_tag="VFG22_06505"
     CDS             complement(21017..22375)
                     /gene="murD"
                     /locus_tag="VFG22_06505"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HET6415928.1"
                     /translation="MRVELAEKKAMVVGLGESGLAVVRFLRAHGARVRVNDRRDAEAI
                     GDAATEAERLGAELVLGGHPEAAFEGLDLIVVSPGVPPLPVLDLAERRGVPVISEVEL
                     AARFIDAPIVAITGTNGKSTVTTLIGEMAARLGRPVFIGGNLGRPMIEAAGSDAGSEQ
                     GLVIVELSSFQLERVSQMKAHLAVLLNVSADHLDRYPSFGDYAAAKARIFERQTERDF
                     AIVPADAPDIHELIKNGGTVVLFGGRGGEVRVVDGALVDTRSPLRVPISELKIRGSHN
                     VSNACAAALAARLLGVPAEDIASTLREFGGLPHRMQFVRSIDGIEYIDDSKATNVGAS
                     VASIDGLAGLDGKIVLIAGGVDKGGSYEPLRQRMLDRGRAIVLLGEAAPLIERAFADS
                     SLDSRRASSMAEAVGQAAALAQAGDVVLLAPACSSFDMFRSYAERGDQFQRAVQSLGE
                     QA"
     gene            complement(22372..23499)
                     /gene="mraY"
                     /locus_tag="VFG22_06510"
     CDS             complement(22372..23499)
                     /gene="mraY"
                     /locus_tag="VFG22_06510"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HET6415929.1"
                     /translation="MIYYLLYPLHTYESLGFLNVLRYVPFRFLMATMTSLLLSFGLYP
                     WFIRRLQRKQIGQVIRSDGPETHFSKAGTPTMGGALMLLALVLSTVLWADPTNAMVWV
                     VTVITVAYGVVGYIDDTSKIRTRSTGGLAGRYKLLLQFSMAILVCAWLYYGETGLPRD
                     WLEIRHRLAAPFFAFSKYPIELPPWFYVIFSSFVIVGTSNAVNLTDGLDGLAIGPVMI
                     SAGTYAILAYLAGLTLFGNVVANYLDIPHIESAGELSIFAASVVGVGFGFLWYNTYPA
                     QVFMGDVGSLALGGGLGALAVLTKNELLSVILGGIFVIEAVSVILQVGYFRMTGRRIF
                     LMAPIHHHFEKKGWPEPRVIVRFWIIAIMLAVFSLATLKLR"
     gene            complement(23496..24902)
                     /gene="murF"
                     /locus_tag="VFG22_06515"
     CDS             complement(23496..24902)
                     /gene="murF"
                     /locus_tag="VFG22_06515"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HET6415930.1"
                     /translation="MATPIPINTAGFRRDQVLSATGGSATGPDWESVSGVVIDSRAVQ
                     PGNLFVALRGERFDAHQFAARAAEAGATAVLVERGTSVPDAVSAVLVDDTLRALGDLA
                     HAHRLAWNGQLIGITGSVGKTTTKDLTAAALESAGRRVLKTGGNLNNRIGVPMTLFRL
                     DSTVDTAVVEMGTSEPGEIARLAEIASPDVGIVTRASLAHTQGLGSVEAVADEKVSLL
                     QALGRDGIAIAYGDDVPVKKRAAVVAAKRKLFYGRTAGNDVRIIDWDVHAGGTRAHLQ
                     VRGQELEIEMSLLGEGAVLNAAGALAIAFALQLPLEDAARGISQVSASPGRMEPRTGT
                     AQRLVIDDTYNSNPASVEIAVDTARMIASKRNAPLVVILGDMLELGTHSEEAHRLVGE
                     LVSNAEVFLFVGCGKGMHIAVTTANARGTDTLWFEDASECGELSDRLPLNAVILVKGS
                     RSMEMERVIAPLAKGEQR"
     gene            complement(24902..>25677)
                     /locus_tag="VFG22_06520"
     CDS             complement(24902..>25677)
                     /locus_tag="VFG22_06520"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HET6415931.1"
                     /translation="RVSVPVTRCSRRPESNAEIIASEWSVTRAGIRAEVDTPAGSISI
                     RSPMLGEHNLENILVALGCAHALGLDLKAAAAAWEAASGSPGRLERIPHPRDVAVLVD
                     YAHTTDALHRVLQTMRAVTPNRLIVVFGAGGDRDRGKRPEMGRVGIQGADLCVLTSDN
                     PRSEDPQVILDELEVGAKQAGGQRIAPRDLVGASRGYCVLADRKEAIRAAIGAAESGD
                     TVLLAGKGHETYQIIGSERTHFDDREEARTAIANLKGPS"
BASE COUNT         5045 a         8085 c         7811 g         4736 t
ORIGIN      
        1 gatggctcta cgcgggcatc tgacctgccg tccgtgctat cctgctgacg tgcattggat
       61 aggggtggtt ctggcttgca cgctgtggct cacggcaacg acgtcattcg ccgatgcggc
      121 gaatgcgcac taccaacttg cgaagacctt gcgcgcagaa ggacgctacg aagaggctct
      181 cgccgaagtc gatctcgccg tggcggagcg accgacctat gcccagggac atctcacccg
      241 aggctcgatt cttcgtcgat tgggtcgcta tcaagaagca ctgcaggcgt tcgagcgtgc
      301 agtccaactc gagcctgaag atgggggggc acaaggtctc accggcgcag ctctcatccg
      361 tctcgagcgt cccaaagagg ctgttccgta cttgaagcga gcaagcgagc tcgagcccaa
      421 gaacagcaac cactggctga atctcggcgt ggcgtaccga aaatccggcg acaacgatgc
      481 tgcgatcgca acctatgaga aggggctcga ggcgattccg aacgacccgc agatcctcaa
      541 caacctgggc gtggcgctgc gcaaggcgcg ccgctacgac gaagcgatca aagcgctcga
      601 gaaagcgagc gcagccgctc cacacgatgc cgaggtcgct cgcaacttgg ccatcgccta
      661 ccgcggagcg aagcactacg ggcaggcggt tccgatctac atccgagcgc tcgagctcgg
      721 ccgtggcggt ccgcccgatc tcttgttcga tctagcctcg tgctacgaaa agctcgggaa
      781 cgacgaaatg gcgatcgcga ccttcgagcg ctacatcgca gcaacgaacg agagcgatcc
      841 ggctggcgcg aatctcgcgc gacgtgcggt cgagaacctc aagagcaaat agcgagctct
      901 gcgccgcggt cggcgcatct tcgcagactc agtcgatgta gaagctgaag ccggcctcga
      961 tggtccaggc ctgatcgggg gcgtcggtcg gcatcttgaa gtagcgcatc cagcgacctc
     1021 gcacctcgag gcccatccac gagagaaacc catatccgag cccgacaaag gcgctgatcg
     1081 tgccgctccc ctcgacgcta tcgggcaagg gaacgcgaag gtatccgccg ccaaaggcgc
     1141 ccgagagcct cagtttcttg atgttggtgc ggagcacgat cactccgccg ccgcccaggc
     1201 ccggctcatc gagcggccag tcttttccga gccactggaa atagccaccc aagtgcaggg
     1261 ggccggccac tcggtaggtc gcgtcgagcc agaggcttgg ctgccatttc cgttggccgc
     1321 tgtacttcga gaccgttcct ccgaagaccc cgtcgatcac gacgagatcg ctgtccgcag
     1381 atgcggtcgg aaccgggccg aaggttcccc acgaccccag caagagcaat gacagcaata
     1441 ctggcgcgga cgcgaagcgc ttcacgccca ggtctgttac cacaagtccg agtgcggtcc
     1501 gcaaaacctg cgaaccggtc gagattcagc ccgcggtcgc ccgaatgatc acgtccgaca
     1561 catcgaattt cttgaggaat tcggtgcgcg cttccttcat gtgcctgacc cactgttcgt
     1621 agtccgcgtg ccgtccacgg ctatcgaaga aacggcgggg ttcgaggatc tcagcgggca
     1681 ccttcgacac cagctcggcg ttttcgggag cgtcgacatc gacgatctcg taaccgaagt
     1741 ctggatccgt cttccatttg acggcgtcgc tcaagagcgc ttcgagcatg gcgctcgagt
     1801 gcggaatctt gatgttcagg gccttgttct ctttggcgtc gttcgcctcg ccgcccacgt
     1861 accctgtgtt catcagccac atgctcacgc cgggcatggt cgccgcgagc tcggcaaatc
     1921 gcttcgcttg aagcccgtgc ttggccggga agaacggctg ggtgaacggc gagcgcgtct
     1981 tgccgcgctc cttgccggcg gcgctggtct tcgatgtctc gccgagcatg aagtagccgg
     2041 ctgcctgctc gggcgaggtg aaacgcacga tgccgggcat cgcggcatcg tagccctcgt
     2101 cgcgattgag gattcccatc gacttgacct cggggatgtt ctcgaggtct gccgcgtccg
     2161 gaatcgagct caagggaacg atagagcgac cgttctgggt ccagacgacg aagtcccaac
     2221 cgtcggcagg aacgccgtga gagtcgacga tctcgtcgat attgaccccg cccgcaatgt
     2281 acttgtcgac cttgccggca tccgcgcccg ttgcaatcaa gatgtcgcgc agacgcttca
     2341 catcctcgat ggacagcacg cccttgctga agttgaacga tccatccgcc tcgaggaaga
     2401 cgttttcgac ccacgcatcg gggccgatcg tggacttgta aagaacgggc tcggtctcga
     2461 gtgaaaggcc gtccgtcttc gcgaagcagc cgttctcggt gatcgagatg gcgccatgcg
     2521 gccaaagcga aaccatgtcg tcctgaatgg gttccgtgag gtcgccctgc ttcgagaagg
     2581 tggtcgtggt cttgcccgtg cccgagagcc ccatgatcgt catggtgagc ttattgccac
     2641 tcttgggggt gacggccttc gcgcctgcat gaagcacgag gcccccttgc tcgtacacgt
     2701 agtgattgag catgcgaagg atgcccttct tcgattcgcc gaagtagtcg gcacccatca
     2761 cgtacgtggt ccagttctcc atatcgacga tgatggcctg tcggcctggc atgtcatcga
     2821 gcatcaggtc cggcgtgtag aaaagacgaa acgtgggctc gaaaggctca gccaacttcg
     2881 ccttactttc cacggcggaa cgcgggaacg cgaggacctg ctgcataccc gcgatgttcg
     2941 cgccctcgag agtataaagc caggtcgtcg aaaacgcgcc gcgcccgaga ccggaatagc
     3001 cgtcgatggc gatcagcttc cccttgtcag cgatgtactt cgcttggcgc tcaatcaagc
     3061 cacgcgcctt agccggatcg atgatctgat ggctccagcg atcctgagtg tcgatgatgt
     3121 acgtgtactt cgccatccgc gctttgttgc gcgtaacttt gttgatgctg cctaccgacg
     3181 ttttttgtac gcagggcgtg tgtgcgagcg ccagctcgcg cagctcagcc tgggttggat
     3241 tctcgatgta ctctacctcg aaggggcggg gatctgcggt cggcatattc tctccgtttt
     3301 cgccttggcc actgggagca aaagacccat tcggcaagct ccggaactgg catatgacaa
     3361 tgtttctgga aggtgtccag gccaacgaga gttgccagtc acgcgacacc aactaagctg
     3421 cacgacgtgc gcgccctgcc gctaaatttt cgcgacgctg agggtttttt cgatccggat
     3481 tggagcgatt ggcgttggca agcgcgcaac tcggtcacga atttacaggg tctcgacaaa
     3541 gccttggatc tcacagacgc agaacgaatc ggcgcggcgc gcgcgatggc cgcgggactt
     3601 ccgatttcga ttaccccgta ctatctggcc ctctgcgacc cgaaagaccc cgactgtccc
     3661 attcgacgtc agtgcgtgcc gcgcgccgaa gaagcgatcg cggtcaaggg cgacctgcgc
     3721 gacccattgg gtgaagaagc tcaccaagtc gctccgcatc tcatacagcg ctatccagac
     3781 cgcgtcctct tgctcgcgac ggatcgctgc ggtgtgtact gccgtttttg cacgaggtcc
     3841 cgactcgtgg gcgacggtgg aggggcgcgt tccatgaccg cgctcgaacc ggcatttgcc
     3901 tggatcgagg cacatcccga ggtccgcgac gtcatcattt ccggcggcga cccatgcgtg
     3961 atgtcgacgg agcgcctgtc ccgcctgctc ctgcggattc gcgagatcga ccacgtcgac
     4021 tacgtgcgtt tggcaacgcg ggcgccggtc actcttcctc aacgaattac cgaggagctc
     4081 gcggcagcga tccgtgaaag tcacgaatcg acctggatta tgacgcattt caaccatccg
     4141 aaggagctga gcgaagaggc gcgagccgcc tgcgcccgac tcgtcgacgc gggccttccc
     4201 gtgatgaacc aaaccgtcct cttgcgcggc gtcaatgacg accaaaacac cctcgaagag
     4261 cttttcaggg gcctggtccg atcacgtgtc cggccctatt atttgttgca gatggatcca
     4321 gtcggaggaa ccgggcacct gcgaacgcct ttgcggcgcg ggatcgagct gatggctgcc
     4381 ctccaagggc gcatgagtgg tatcgcgctc ccaaagctca tcgtcgatac tccgggtggg
     4441 ttgggcaagg ttccgattgg accgaattac ctgcggagcg agcaagacgg tgtcacggtg
     4501 ctcgagacct ttcgcggtga tctcgtggag tattacgatc ccctcgagct ttgaaacctc
     4561 tgctttttcc gcccgccgcg tggcaggcac tggccggtag atgtatgaat cggccgctcg
     4621 gcgtaccggg cactgaggag aaaccatgca acgagccttg acgctgcatc aaacgaccat
     4681 cgggaagaaa gtgatcatgg ctctgtcagg cctgatcatc gtcggtttcg cgatcggtca
     4741 cttcctgggc aacctgaaca tctacctcgg ccaggaagcg ttggacggat acgccgccac
     4801 tcttcaaagc ttgccgccgc tgatctgggg aacccggctg ctgcttctct tcgcgtttgg
     4861 cgcgcacatc gcctcggcct tctcgctctg ggtgcgcaac atgaaggccc ggggttcgcg
     4921 gtacaagaag catcaggacc tcgcgaccga ctacgcagca agaaccatgt actggtccgg
     4981 tccgattctc ctgctcttcg tcgtctacca cttgcttcat ttcaccattc ttccggcgca
     5041 ccccggagag gtgtacagga acgtcgtcga gggttttcag gtcccgctga tcgccggcgt
     5101 ctacatggtt ggaaacatcg cgctcggttt gcacatctat cacggtatct tcagcgcgct
     5161 ccaaacgctg ggcgccagtc atcctcgcta caactcctac cgccgcgatg ctgccatcgc
     5221 gatttcggct gcgatcacga tcgggaacct gtcgttcccg atcgccgtgc tcgcgggatt
     5281 cgtcacactg taaaggtcgt catggaactt cgatcgaacg aaccgaccgg tcctatcgag
     5341 aatcgctggg cacagcacaa agaaaacagc aaactggtcg cgcccgcgaa ccgccgcaag
     5401 tacagcatca tcgtcgtcgg cagcgggctg gcgggtggtg ctgcggccgc ttcgctcggc
     5461 gagctgggct acaacgtgaa gtgcttctgt tatcaggaca gcccgcggcg cgctcacagc
     5521 attgctgcgc agggcggaat caacgcagcg aaaaattacc aaaacgacgg ggacagcgtc
     5581 tatcgactgt tctacgacac ggtcaagggc ggtgacttcc gctcgcgcga gtcgaacgtc
     5641 taccgattgg ccgagctcag cgtgcaaatc atcgatcaag ccgttgcgca gggcgtccca
     5701 ttcgcgcgcg aatacggtgg cctcttgggc aatcgctcat ttggcggcgc tcaggtttcc
     5761 cgcactttct atgcacgcgg acagacgggc caacagctct tgctcggcgc ataccaggcc
     5821 ctcagccggc aaatcgaggc tggcacggtc gagatgtttc cacgaaccga gatgctcgac
     5881 ttgatcgtcc atgacggccg cgcgcgcgga atcgtcacac gggacatgac gacgggcgag
     5941 gtcagcccgc atctcgcaga catggtgatt ctgtgcactg gcggctacgg caatgtgttc
     6001 tacctctcca cgaatgccaa gggctgcaac tgcatggcga tttggcgtgc acaccgccat
     6061 ggtgcggcat ttgcgaaccc ctgctacacg cagatccatc cgacctgtat tcctcaggcg
     6121 ggcacctatc aatcgaagct cacgctgatg agcgagtccc ttcgcaacga cgggcgcgtc
     6181 tgggtgcccc gcaagaagga agacgtcacc aagcgacccg aagacatccc agaagccgat
     6241 cgctactact atctcgaaga gcggtacccc gcgttcggca acctggttcc ccgcgacgtc
     6301 gcttcgcgca acgcgctcta cgtgaccaac gacggactcg gcgtcgggcc cgaagtcgac
     6361 ggcgagcgcc gcggtgtcta cctggacttc gccgatgcga tcaagcgcct gggcgagcaa
     6421 gcgatccgcg aacgctacgg caatctcttc gacatgtatg agcggatcgc gggagagagc
     6481 ccgtacaaga caccgatgcg catctacccg gccacccact acacgatggg tggcctctgg
     6541 gtcgactaca acctcgaaac taccatcccg gggctcttct ccggcggaga ggccaatttc
     6601 tccgatcacg gggcaaaccg actcggggcc tccgcgctca tgcagggctt ggccgatggg
     6661 tatttcgtgc tgcctaccac agtgggcaac tatctcggcg aggtacgcac ccttgcccct
     6721 ctcaccgaag accatgacga cgtcacgcag gccgtgaagg ccgtcgaaga gcggattcac
     6781 aagctcatga acgctcccga tcccaaacac gcaccggagc acttccatcg caagctcggg
     6841 cggatcatct ggacaaagtg cggcatgtcg cgaaaccggg agggtctcga agaggcgcta
     6901 cgcgagattc cgaagctgcg ggaccagttt tggcgggagg tgaagatcac cggcaccggc
     6961 accgagctca accaaacgct cgagcgcgcc ggccgcgtcg ccgatttcct cgagttgggc
     7021 gagctcatgt gcatagacgc cctgaagcgc gaggagagct gtgggggtca tttccgcgag
     7081 gagcacgaag aaaacggaga ggctctccgc gacgacgaga acttcaccta cgtggccgcc
     7141 tgggagtgga ccggaaatcc cggacagccc cggctcaaca aggagccact gagcttcgaa
     7201 tacgtcaagc ccagcaagcg gagctacaag taaggggcct atcgatgagc gacgaactac
     7261 tcaatctcac tctccacgta tggcgccaag acggccccaa cgagccaggc cgcttcgagg
     7321 cctaccaggc gaaggacatc acgactcaca tgtcgtttct ggagatgctc gatgtcgtga
     7381 acgaggaact gatcgaagag ggcaagtccc tgatagcctt cgaccacgac tgccgcgaag
     7441 gcatttgcgg gtcttgtgga atggtagtca acggcatggc gcacggccct caaaggatga
     7501 cgacgacctg ccaggtgcac atgcggcagt tcaagaacgg cgatgagatc tggctcgagc
     7561 cctggcgtgc gaaagggttt ccgatcatca aggacttgat cgtcgatcgt tctgcgttcg
     7621 atcgcatcat ccaagcgggt ggttacatct ccatccgtac gggaagtgcg cccgaagcca
     7681 acgagatgcc catcgcaaag gaaatcgcgg accaggcgat ggacgccgcc gagtgcattg
     7741 gatgtggcgc atgtgtggcg gcctgcccca acgcatcggc cagcctcttc acggcggcga
     7801 agatctcgca tctctcgctc cttccgcagg gacaagcgga gcgccaggac cgggcgcgga
     7861 agatggtcgc tcagatggat gcggaaggct tcggcggctg caccaaccac ggcgaatgcg
     7921 aagcggcttg tccgaagggc gtgagccttc gctggatcgc gcgtatgaat cgcgagctcg
     7981 tgcgctcgat ggtgaaagcc gatacgccga tcgcgagccg cgagcgctag gaccgccggt
     8041 cgtcccagac gaaccgggca gccaccgcga cgatcgctag gatgacgaag accaaggtcg
     8101 cgatcgtgta gcgctcggca aggtcgctca aggcctccca gttgctcccg accgcatagc
     8161 cagcgccgag cagcatggcg ttccaaagcg ctgccgatat cccgccgtag agaatcaccg
     8221 cgcccaggct catccgggag agcccagccc cgacgaagaa gaaggcacgt agcgccggca
     8281 agaatcgatt cgccgcaagg tacatcgctc cgtgccgctc gtaaccacga cgcactgcgt
     8341 cgagagcgcg caccgccttg ggcgacttca gaaaagacgg ccaactctct tcgtgtcggg
     8401 cgagccaagc accgaacccc caggccgcga ggccgccggc catcgctcct agggtcatcg
     8461 agacgtacac gaagacccaa ttcaattgcg cgcgagcggc caacgcgact gcgaagagcg
     8521 caaccgtgtc cccagggatt ggcgggaaaa cgtactcgat cgcggcggac gcagcgacga
     8581 gcaggtacga ccagtacagc ggaccgtgct ccaatgtgtg cagaaattcg tcaagcatca
     8641 gaacagcaca taggcttgcg atcaggtttg gtgtagcaca ctgctcgact agacatcagc
     8701 gctcgatgag tcaatctact tcaacgcaag gttagtatcg tggcgagtgg agatcctttg
     8761 gtgtcagtcc ttcaacgtcc caacgcacgg gtcgaagacg tcgccatcct catcgccaaa
     8821 gaagccaacc cagtgctcga cgagcatgcg atgcgtcgta gcctcgatgg cctcggggcg
     8881 gaggtattga ttcgtcgagg cctccgctcg accccggaac gcgacggacg aatcctcgcg
     8941 gagttcctct tccaggagct cggtttcgaa ggcgagctcg acgactacta caacccctac
     9001 aacagctacc tcgacaaagt gatgctgcga cgcaagggga ttcccatttc gctttcggtt
     9061 ctcacgatgg tggtcggtga acgtgcgggc atggacgtcg aaggggtcgg ttttccgggt
     9121 cacttcctgg tgcgcgtggg cggtccaaac ggcgtctacc aagatccctt ccatggcgga
     9181 gagctcctcg acggcaatgg cttgcgaagg ctcgcagctc agttcctcgg agtcgagatg
     9241 gcactgcacc cgagctatct cgagcctgtc gactgtcata cgatcacgat tcgcatgctt
     9301 gcgaatctga agaacgcaca tcggcgacaa ggcgactacg cgcgcgcaat gcttgcctgc
     9361 gaccacctgg tggagctgac gcaggcgccc gagcaccgtc gagaccgcgg cttgctcgcg
     9421 cacgccctgc gcactttcgg agctgcctcc gaggacttgg ccgcctatct cgaggcgcgc
     9481 ccggacgcca aggacgcggt cgcgataagc ctcgccctcg aagaagcgcg gcagcaggcc
     9541 gaccagtttc tccattgatt tgaggcggcc actttacacc aggccatggc gtgcaatgac
     9601 tcggcccgac cggtgagtcg tatgtcgatc gaatccctca aagcgctgtt tcggccggag
     9661 ctcgcagagc tcactgcgta ccgggtgcct cccgcggcgc ccgcggtgaa gctcgacgcc
     9721 aacgagagcc cattcgagct gccccccgag gcgcgggcac gaatcgcgac catgctgcac
     9781 gcgaccgaaa tgaatcgcta tcccgacgga cgcgcgaccg cgttgcgcaa cgctctggcc
     9841 cacggcctag gcgggagcgc ggatgagtac gtgcttggag ttggttcgga cgagctcatc
     9901 gcattgttgg cgacggcaat gtcgacaccg cgcgccggtg cacaacgccc cgtcgtcgcc
     9961 ttccccgacc cgacgttcgt gatgtatgcc atgacgagtc gtgctcacgg ctgggagccg
    10021 gtcggcgtac cactcgacga cgactgggac ctcgacacga atgcaatggc gagggcgctc
    10081 gaacgccgga acccgaagat cgcctactat gccaccccga ataatccgac tgggaactgc
    10141 ttttctcctg agaagattga gctcttggtc gacgcttttc caaacacctt acacgtcatc
    10201 gacgaagcct acggggcgta ctccgagcag tcgttcgcga cattcgccga ggagcgagct
    10261 cactgcgctc tcatggggac cttgtcgaag gtcggcttcg cgggcatacg cgtcggctgg
    10321 cttcgtatcc acgaggcgct ctcggcagag ctcgagaagg tgcgccagcc attcaacttg
    10381 aacacggcgt ctcaggtcat tgcgacgctg gctctgacgg acctcgcagc cgtgatcgaa
    10441 gagcacgtca ctctagtggt cgcggaacgt gagcggctgg cgaagcagat cgacgcgtac
    10501 caggagcttc actgcttccc gagcgacgca aacttcctgc tcgtgcgata cacgggcgac
    10561 gttcgggagc tctcagctgc gctactggat cgagaaattg cagttcggct gtttttacac
    10621 ggtgatctgc ggctccaaag ctgcttacgg atcacgattg gcacccctga tgagaatcag
    10681 cgtctcctag aggcgctaag cgatatacta ggctgagcga tgtccgtagc gcgagagata
    10741 acgcccgagc gcgccgtcga ggctttgaac tgggcggcca acgtggccga agacccgacg
    10801 ctgctggagc cgcagcccga gcgccctacc ccggcggaat ggcgctcggt tccggtgtcg
    10861 gactttcgcg cgcagatgga ccgcctgctc acagcgaagc atccggagcc aggcctcgac
    10921 gcgatcgagt ccctgggctg cctcgacgtc tggttgccgg aagtcgccgc catggtcgga
    10981 ttcggcgaca acgagtggcg tcacaaggac gtctggaagc acacgaagca ggtggtgaag
    11041 cagtccgttc cacgcccgga agttcgttgg ggcgccctgc tccacgacat cgggaaaccg
    11101 aaaacgcggc gaatcgacga gcgcggcaac gtgaccttcc atggtcatgc ggaagtcgga
    11161 gcttccatgt tccgaaagcg cgtggccgag cgcatcggct tcgagggcgc gcagcgcgag
    11221 cgtgtccact tcctgatttt gcaccacctt cgcgctgctc agtacgacga ggactggacc
    11281 gacgcggccg ttcggcgctt ctacaaacag atgggcgacg gcctgcgcga tttgctcgat
    11341 ctcagccgtg ccgacatcac caccaagcgc cccgaaaagc gccggcgcgg cgtgcgccag
    11401 atcagcttgc tcgcgaaacg cattcgcgac ctgcaggaag aagacagcaa ggtcgcgcca
    11461 ctgcccaagg gtttgggcgc ggtgatcatg agcgagttcg gcgttccgcc ttcgaagcgc
    11521 ctcggcgacc tgatgaagca actgaccgcc gccgccgagg ccggcgaggt cgagcgagag
    11581 caatccgcgg agtactacgt cgagtacttg tccgcacacc gggccgagtt tggtctgaac
    11641 gactgacccg cgccgtcgcg ggcgagcgtc acgggcgccg tagctgaaat tccgcgacgg
    11701 cgtccatgac ctcgtcgaag tcgtcctctc gaaacccagc tccactgacc atccaatcgg
    11761 tgtgcgcgga gtcccggtcg ccatgaaagt ggccgaggat atcgagatgg tcggcggtgc
    11821 cgccccagag gacatcgccc caaatcatgc tgccggtggg gacaaggcca tcgttgaggc
    11881 tgttgtctag cgttcgtccg agggccgttt ccaagtattc ccgcacctcg ggggccgggt
    11941 tgggaggcgg atagaccttc gacgtgcgac ccgctccgac gtagagcgtc gagaagatca
    12001 gcgcagagag caggctggca ggcgagcgca gatcccggat caggcgcacg ggcgctggtg
    12061 cgggcatccg agtgaccaca cacccgtatc gaatgttcgc gttgtcctca atgcctgcgt
    12121 tgaagatgtc catcgactcg ggggtgagct gaatgatggc gccctgatcg ccacgaatgc
    12181 cttcgatcca ttcccgtact tccgcctgcc cttcgttgcc gaggaacttg agcaggagct
    12241 cggtggctcg ctccagcagc ttgatctcga gcccgagcgc ccggtccatg tgacccagcg
    12301 aggcgatgag aggagcgagc acggtgagtg ggggtccgcc ggcgcgcaga gtgaaatacg
    12361 tgaggagcga gagcgcctcc agcaattgcg tgcccgccaa cgtcgtgagg aagtacgcga
    12421 ggggtgtgcc gtagtggggt gttgcgatgc tcgtgaccgt tcttacgcga tcgagccata
    12481 caggagcctc gggggacccg ctgggtgaag ccagaagtcg gacgtcgagt ccaccggtcg
    12541 aatgtcctac gagatgaatc gcgccggaat cacccccaca attccgctcg atcgcatcga
    12601 ggagaacctc ggtgcgatac acgatcgaac ccgtcggggg agacgagacc agcacgaact
    12661 cgcaatccgc gccccgctcg aaaaagcggc tcgcgagtgc ttcctcgaca tgcatgaagt
    12721 agtcgaaccc accgagcttc gcaaagccga agaggccagg cacgagcacg atgtgatggc
    12781 gagggcgcgg catgaaaccg gactctacca tgcagcgaga tcagcccccc gtctcgcggg
    12841 tgctcgcgcg ccttagtggc ggtacgaaag caagacgaca ccgccagggc cgcgttgtct
    12901 cacacgcaac tgaagcttct cggtgtgtgg cagctttctc tgattgaggt cgaccccgta
    12961 ccgcgcgagc ttgtcgaccc gtgccgcgag gtcgcgaatg tcacgcgaga gcaagaaacg
    13021 aatcacccga ggcgtgcgcg tcgcagccgc gatcaggtac gggttgttga gcccgagcac
    13081 tgagataggc aacgctattt gggtcgtgcg gatggccggc agcggactct cggccgaggc
    13141 gctcttcgcg ccgaagatcg aggtgatgaa ggcgcagaga aagcagaaca tcccaaaaca
    13201 tgggcacctg cgacgggcgt ctagccccac ctgaacactc ttcttcacca tgagctcttt
    13261 caaactatgc ctgagagcaa aaaaataagc tagaggggtc aattacgaac gggaacagcg
    13321 agccctcaca tgctggaagc tacccacttt caccaagctt gcaaagtgaa ttgggctcat
    13381 ttttggtaac tatcgggtga cgtcaccgca cggcgccccc aggagcgagc tgcaggcggc
    13441 gagtgaaaag cccggacaat gccgggcttg aagctaagtg tatgaaataa ctcttaactc
    13501 actcgacggt gcggtttccc ctgcgcaagt aggccgggat gtcgaggacg gcttcgtcgt
    13561 gaagggcgct cgcgccgaaa gccctggcgc cagccatctg cggtacgacc gccggcacgc
    13621 cctcgagcgt cgaagagagg tcgtctcctc gcgcggccac agttcgcata gccctattgg
    13681 ctgaggagcc gttagagctc tgattgctgg aatgagcgtg gcgaaggccg atcggcatgg
    13741 gcgcgtggcc cgatttttcg aagccggtcg cgatgacggt gaccttgacg gtatcggtga
    13801 ggttggggtc ggtcaccaca ccaaagatga tctccgcgtc ctcgtgtgcg gcctgctgaa
    13861 ccagaccggc cgcttcgttg atctcgcgga gcttgatgtc gggacccgct gtgaagttga
    13921 tcagaatgcc ggtcgcgcct tgcaccgaaa cttcgtcgag gagcggggag ttcaccgcca
    13981 tttcggcggc ctcgagcgcc cggcgatcgc ccttggcgat gccagtgccc atcaatgcgc
    14041 gcccattgtt cgacatgatg gtcttcacgt ctgcaaagtc gacgttgatc atgccggcgt
    14101 tgatgatcag gtcgctgata ccttggacag cctgcaggag gacgtcatcg gcacgtgcga
    14161 aggcgtccag catcgtgatg tcgtcatcgc cgagcgacat gagtctctgg ttgggaatcg
    14221 tgatgatgct gtcgatgacg tcgatgagct cctgaacacc gcgctcggcg ttcttggaac
    14281 gcttgcgccc ctcgaacagg aagggcttcg taacgacccc aaccgtcaac gctccctgat
    14341 cgtgcgcaac ctgggaaatg atgggagctg cgccggtacc cgtgccaccc cccataccgg
    14401 cggtgatgaa caccatgtcg gccccttcca acgcttctga aagacgctca acgtcttcga
    14461 gagcggcctt gcgaccgacg tcgggattcc cgcccgcacc caggccgcgt gtgagctcgg
    14521 ggcccagctg aattttcact tgagcaaggc tgcgatccaa cgcttgcgcg tcggtgtttg
    14581 cagcgatgaa ctccacgccc tccagaccac gttggatcat cgtgttgagt gcattgccgc
    14641 cactgccgcc aacgcctacg actttgattc gtgcctgaag ctctgcgtcg tccgcgaact
    14701 cgatcgagat agccatcgat tcctcctaaa aaacttcctt gaaccacgcg ccgatcttgc
    14761 gaccgagtcc acgactcgcg gccacttcca ctgttcgatc gggcgcagac ggtcccgcca
    14821 attgctccgc tccgtgtttc acgagtccga cgccggttgc gtaggacggg ctcttcacga
    14881 cgtctatcaa tccaccgatg cccgtgggtg caccacgccg cactggaagg ccgagcactt
    14941 cctcggcaag ctctggtaag ccatcgagga gcgtcgttcc accggtcacg atcgctccag
    15001 acgccaacat gtccatgtag ccggtctcgg caattacatg gcgacatgcg gcaaagattt
    15061 cttcgacacg aggctcgatg atgtcgcaga ggaccctgcg cggcagcgag cgcggtgccc
    15121 tacccccaac cgaaggcacc tcgatcgtct cgtcgtcgtc gaccatgcga gaggcagcgc
    15181 acccatattt aatcttgatg cgctcggcct cggccatggg cgtgcgaaga cctgttgcga
    15241 tgtcgctggt gagattgatt ccgccgattg ggatggagct cgtgtgcacg accgcgccgt
    15301 cgacgtagat gatgatgtcg gaggtccctc ctccgatgtc gatgagcacg cccccgatct
    15361 cacgctcgtc actgctcaac acagcatgtg cgctcgcaag cggctgaagc acgatttctt
    15421 ccacgaagag accgcagcgc tcggcgcatt tcgtaacgtt cgcgacgctg gtcgaggcag
    15481 cggtgaccat gtgcacgcgg gcttcgagac gaactccggc catgccgatg ggctccctga
    15541 tcccgtcttg ttcgtccacg atgtactcct gcgggagcac gtggaggact tggcgatctc
    15601 cggggagcgg tacggccttg gcctgctcga gcacccgcat cacgtcctcg gcggaaacct
    15661 cacgattcgc aatggcagcg accccgtcct ggttcatgcc gcggacgtga ctcccggcga
    15721 tgccggcgta caccgcgttg atctcgacac ccgccatcgt ttcggcttgc tcgactgccg
    15781 cccgaatcgc ttggacggtc gactcgatgt tgacgacgac gcccttcttc aaaccctttg
    15841 aaggaacgga gcctatcccg atgatgtcta gaccttcttc cgacacctcg gccacgatcg
    15901 cgcagacctt cgtcgaaccc agatccaacc ccgcaatgat ctcgttttcc gccatccctg
    15961 cctctgactg acccataccg aaacgggatt tggcaggaca acttttatgc agaaaaaaat
    16021 ggggtgaaac gcgttttttc ttcctgggag tttaacccct gcgcgaagtc gaacccgacc
    16081 tcggacctca agggagccgc accgtcacgc gctcaggact tcgaacgttg tcgaggtata
    16141 cgtaggaagg tcgcgttttc tctcgctcaa gtcgttcgag gacttggcgg aggcgacgga
    16201 gcttttggcg gtgttggccg ttgccaagtc ggacgttcat gccgtcgtcc ccgacgaaga
    16261 gctcgattcc gtgggcgcct tcaaaatgaa tttccgaaac cggctcgcgc tccgccaggc
    16321 ctgctccttc atagtcctga agcaaggcca tcgatcgcag gaggatcgcg gtgcggtagt
    16381 ccaagtcgtc gtagaacctt tccgacgcga tcccggtgat gaccggcaag tcgactgggt
    16441 cgtcgacgcc gaggcgcttg aagaccgccc cctcttcgct cacgagatac agttggtcca
    16501 aggccaccag cgctacgggt ttgcgctcga cgatttcgat tcgaacgcgc ccgggtagcc
    16561 ttcgaacgac gttcgcttcc tcgatccatg ggtgctgaag caagtgattc cgagttcctt
    16621 cgctcgaaac ctcgaaaata tttgaaccta tctgcaggcg agcggcccga cggacgtcga
    16681 tctcgccgag ctgatggttt ccttcgatga tgatctcgcg gatctcgaac gcctcggcgc
    16741 tatgagcgta ctgcacggtc cgatcgccca accagagcag cgcggcagcg ccagccccga
    16801 ggaggatgag ctgaccggta cggcgaaaga gcgaaccgtg agagatgacc caggcgcgta
    16861 ccccctcgca agctcctgcg aaccgttcgc gccagctctt cgtcgacttg ctgcgccgag
    16921 gctttttcgc gaccggccgt cgccgattct gaccacgatc ggacttcgcc gtctttcggg
    16981 gtatcttctt ccggggcgtc gccttgaggg cggttttgcg cgtggtcgct gtggatgagg
    17041 tcttctttgc tgccgagttc tttttgcgca cggggcgact gggggaggtc ttcgactcgg
    17101 ccgggcttcc ttggagcggc ttcttcggcg ctggtttctt ccgcgcactc gatggggagc
    17161 gccgacgatt catcgccgga gcacctcgag gagttcaggg ccggttcgcg taatgtcccc
    17221 ggcaccaagc gtgatcacga cgtcaccggg ctgtatccgt gttgccatcg cttcggccaa
    17281 gtccttccga tcttccacca aggtcacgtc ccgatgccca tgggcgcgaa tcgcctcggc
    17341 aagtcgacgc gcatctgccc cgtcaatggg ttcttcgccc gcggggtaga cctcggtcaa
    17401 gaataggaca tcggctcggt tgaacgctct ggtgagctcg tcgaagagat cccgggttcg
    17461 cgtatagcga tgtggctgga aggccacgac gatgcgagag ccgtacgcct gctgcgcagc
    17521 ctcgagagtc acctcgacct cggccggatg gtgaccgtaa tcatctatca ccgtgacccc
    17581 agccgcttcg ccgacgatcg agaaccgccg ctgcacgcct tcgaagtccc gaagtgccga
    17641 acgaacctga tcgtgatcga ctcggagctc gtctgcgacg gcgatcgtcg cgagggcatt
    17701 gagcacgttg tggatcccgg gcattcgaac ctcgaactga cccaagcttt tgccgtggtg
    17761 ctcgaggtcg aatcccaccg agaggcccga gaacaccggg ttccgcgctc gatagtccgc
    17821 atgcgccgag aatccgtagg tcacgatgcg cttgtcgagt cggggcaaca catcttgaac
    17881 gttggggtga tcgagacatg ccacgaccag tccgtagaat ggaactcgat tggcaaagcc
    17941 cacgaacgcc tctttcacgt tgtcgaggct tccgtagtgg tcgaggtgct ctggatcgat
    18001 gttggtgatc actgcgatgg aaggaatgag atggtggaag gatccatccg actcatctgc
    18061 ctcggcgacc aggagatcgc cggcgcccat ggcagcccct gaaccgagcg cgttgagctt
    18121 gccgccgatg atgacggtcg ggtccaagcc cgctcctcta aggacggtcg cgaccaacga
    18181 ggtcgtggtg gtcttcccgt gtgagccagc gatcgcaatg ccgtccttga gtcgcatgag
    18241 ctcaccgagc atctcagccc tcggaattac gggcacccct gctctgcgcg cttcgagcag
    18301 ctctgggttc gtaggaggga cagcggagga gaacacgacg acgtcggcgt gcttcacctg
    18361 ttccgcctga tggcccacga agatcttggc tcccttttcc gcgagtcgct tcgtgtactc
    18421 gttctcgcgg aggtccgagc cagtgacttc gaacccggag tcgaggagaa cctcggcgat
    18481 accgctcatc ccgaccccgc caacacccac gaaatggatg gatcgaattc taccgcgaaa
    18541 catggacgct tactcccaca gtgctccgtc gacgacaacc ggccggcggg gtcgatggct
    18601 cgccggtcgc aacgcgactt cagggcagcc aagcctgagg atgggcgcca tggccagtcc
    18661 gctgtattgc aggcccgctg ggggctctct ttcgggctgc gctgcctcag cgccgagcgg
    18721 atccgcacct cccacccaag ccatcatgtc gtcgacgatc gcagcggctg catccggcct
    18781 tccatgatca cgcgccgcct gagccatcgc ttggcgcctg accggatcgt ccaacaagtc
    18841 agcgatcgtt gcacccagcg cgtcgacctc caggtccgcc tcgcgcaggc agatcgatgc
    18901 atgctgctct tcgagcgcct gggcgttctt cgtttggtga tcgtccgcgg cgaacgggaa
    18961 cggcaccaaa atcgaggggc ggcctatcgc gcaaagctca gcgagagtcg tagcaccggc
    19021 acgcgcgatc acgagggccg cgttcgaata cgcacgagcg atttcgtcga tgaaagtcac
    19081 gactcgcgcc tggatgccga gctcctggta ggccgcctcg acctggtcgc gcatcgactc
    19141 tcccgtctgg tgaaccactt cgagcctgcg agccgcgatg ccggctttcg caagcgcgcg
    19201 cggcacgtct tcgttgagct ttctcgcccc ttgcgagccc ccgagcacca agatcgtccg
    19261 cgctcgtgac tcgaaacctt ccgggtcggc caatgcgagt cgcgcgtgct ccacgaactc
    19321 ttgccgaacg gggttgccgg tgagtctgga ctttctgcgc ccaaagacct cttccgtttg
    19381 ctcgaagctg atgtaggcgc gatcgacgaa tctcgcgagg atccgattgg tcatcccgac
    19441 ttgggcgttc tgttcgagca gtgcggtcgg tctgcccgag agggacgcgg agagtaggac
    19501 cggacccgaa gcgtatccgc ctacgccgag caccaaatca ggttcgaagt cgtccatcag
    19561 cgctgaggcc ttcttcatcg ccgttgggat tcgggcaaag ctcttgaagc gggcccccaa
    19621 cccctgtcct ttgagaggcg tgacttcgag caactccaag gattcgccgc gccgcggaag
    19681 gattctcgat tcgatgccgc gtgcggtccc cacgaacctc acctcgatct ctggaagtct
    19741 gcgacgcagc tcgtccacga ccgcgagccc cgggaagaga tgacctcccg tgccgccgcc
    19801 cgccaccatg atgcgttgaa tcatcgtgca cctccactgg cagtccgcag tcgtggctgc
    19861 gcttgcttga ctttcgcttg agctggggac tcttcatcac gagaacgccc gaaacgcgac
    19921 gtgttcaaaa tcatgcccgc ggcagcggcg ttgacgatca gggatgaacc gccgtagctg
    19981 acgaaaggaa gcgcgagccc cttggtcggc aaaagtccca tcgcaacggc caggttggtg
    20041 aatgcttgaa ggccaatgaa gagcgtcatg ccggccgcca ggtaacccgc atattcgtcc
    20101 tgggcgcgcc atgccgcccg aagacctcga aagaccagcc aagcgaaagc ggcacagagg
    20161 agtgtgaccc cgatgaaacc gagctcttcc ccgacgatcg cgctgatgaa atccgtatgc
    20221 gcctcgggca ggaacagcag tttttggcgg ccatccccga gccccactcc ggtccatcct
    20281 ccggacccga agctcatcaa cgactccgtg atctggtagc ccgcaccctg cctgtgctcg
    20341 aagggttcga gaaaggcctt gatgcggcgc atccgatacg gcgacgaagc gatcgcagca
    20401 tagccgaccg gtagcgccag caagacacaa ccgaggagat agccagcctt ggccccggcc
    20461 gcgaagagaa ccacgaaggt cagaagcgcg atcatgaccg cgcttccgaa gtccggctgt
    20521 gcgaggcaga gcagcatcag caaacccgca accagaacgt gaggaaggaa tccgatcgag
    20581 aatgttcgaa cgcggttcga cttcttggtc agagaatgcg caagccacat gatcatcgcg
    20641 agcttcgcca gctccgcagg ctgaacgttg actggcccca gagcgagcca gcgtgtggca
    20701 ccgcctgcgc tgcgtccgaa tccgagagca acggcgatca tcagcaatat cgcgagcaag
    20761 aggacgggat aggtcgagcg tcgaagcagg cctaggttga cgcgcgtgaa gatgatgaga
    20821 accacgaacg acagggcggc aaagacagtt tgccgaacca ggaagtggta tccgttccca
    20881 aacatgttgt tggcgtagac ggcgctcgcg ctgtagacca tcaccacgcc gaacgcgatc
    20941 aagcccacga gcgtggcagc gagcgtgacg tcagccggac caggttcggc cagtggcgcg
    21001 ggattcgagg aacgggtcat gcttgctctc ccagggattg aacggctcgt tggaactggt
    21061 cgccccgctc ggcgtaggac cggaacatgt cgaagctcga gcaagccggc gctaaaagca
    21121 cgacgtcccc ggcttgggcg agtgcggcgg cttgcccaac agcctcggcc atcgagctcg
    21181 cgcggcgact atcgagtgag gagtccgcga acgctcgctc gatgagtggc gcggcctctc
    21241 cgagaagcac gatcgccctg ccccgatcga gcattcgttg gcggagcggc tcatagcttc
    21301 cgcccttgtc gactccgccc gcaattagaa cgatcttgcc gtctaagccc gccaatccgt
    21361 cgatcgaagc gaccgatgcg ccgacgttgg tggccttgga gtcatcgatg tattcgatcc
    21421 cgtcgatcga gcgtacgaac tgcatccgat gcggaaggcc gccaaactcg cgcagcgtcg
    21481 aggcgatgtc ttcggccggc acgccgagaa gacgcgctgc aagtgccgcc gcacacgcat
    21541 tcgacacgtt gtgtgagcct cgaatcttga gctcgctgat cggaacgcga agtgggcttc
    21601 gtgtgtcgac gagcgccccg tcgacaaccc gcacctcgcc accgcgacct ccgaacaaaa
    21661 cgacggtgcc cccattcttg atgagctcgt ggatgtcggg ggcatctgcc ggcacgattg
    21721 cgaagtctcg ttcggtctgt ctctcgaaaa ttcgggcttt ggccgcggcg tagtcgccga
    21781 acgacgggta cctgtcgaga tggtcggcgc tcacgttgag caagactgca aggtgcgcct
    21841 tcatctgcga tacccgttcc aactggaagc tggagagctc gacgatgacc aggccctgtt
    21901 ccgacccagc gtcactgccg gccgcttcga tcatggggcg acccagattt ccgccgatga
    21961 agacaggccg cccgagacga gccgccatct ctccgatcag cgtcgtcacc gtgctctttc
    22021 cgttggttcc agtgatggcg acgatcggcg catcgatgaa ccgtgcggca agctcgacct
    22081 cgctaatcac ggggacgccg cgacgctcgg ccaggtcgag caccggcagc ggtggaactc
    22141 caggggaaac gacgatcaaa tccagccctt cgaatgcagc ctcggggtgg ccaccgagaa
    22201 ccagctctgc gccgagccgc tcggcttcgg ttgccgcatc gccgatcgcc tcggcatcac
    22261 ggcggtcgtt gactcgcacg cgcgcgccgt gagctctcaa gaagcgaacg acggccaacc
    22321 cgctttcgcc gagccccact accatcgctt tcttctccgc gagctccacc ctcacctcaa
    22381 tttcaacgtg gctaggctga acacagccag catgatcgcg atgatccaga aacgaacgat
    22441 gacccggggt tcgggccagc ccttcttctc gaaatgatga tgaatcgggg ccatcaagaa
    22501 gatccgacgc cctgtcattc ggaagtagcc aacctgcaga atcacgctca cggcctcgat
    22561 cacgaagatg ccgccgagga tcacggagag cagctcgttc ttcgtgagaa ccgccagagc
    22621 gccgagtccg cctccaaggg cgagcgaacc cacatcgccc atgaagacct gcgctggata
    22681 ggtgttgtac cagagaaaac caaaccccac gccgacgact gaagcggcga aaatgctcag
    22741 ctcaccggcg ctctcgatgt gagggatgtc gaggtagttt gcgactacgt ttccgaacaa
    22801 ggtgagaccc gccaggtacg cgaggatcgc gtaggtccca gcagagatca tcaccggacc
    22861 gatcgcgagt ccatcgaggc cgtcagtgag gtttactgca ttggatgttc ctacgatgac
    22921 gaatgaagaa aagatgacgt agaaccaggg aggaagctcg atcgggtact tcgagaacgc
    22981 aaagaagggc gcagcaagac ggtgacgaat ctcgagccaa tctctcggta ggcccgtctc
    23041 tccgtagtag agccaagcgc agacgagaat cgccatggag aactgcaaga ggagcttgta
    23101 ccggccggca agtccgcccg tcgatcgagt ccggatcttc gaggtgtcgt cgatgtagcc
    23161 cacgacgccg taggccacgg tgatcacggt gacgacccag accatcgcat tcgtcgggtc
    23221 cgcccaaaga accgtggaca gaaccagagc aaggagcatg agggcgccgc ccatggtcgg
    23281 cgtccccgcc ttcgaaaagt gcgtttcggg tccgtcggat cggattacct gaccgatctg
    23341 ctttcgctgc aaacgccgaa tgaaccaggg gtagagaccg aaggacagaa ggagcgaggt
    23401 catcgtcgcc atcaagaagc ggaacggcac gtaccgcagc acgttgagaa accccaagga
    23461 ctcgtaggtg tgaagcggat agagcaagta gtagatcatc gttgctctcc ctttgctaga
    23521 ggcgcgatca cgcgctccat ctccatcgac cgcgagccct tgaccagaat gacggcgttg
    23581 agcgggagcc ggtcggaaag ctcaccacac tcggacgcat cttcgaacca gagggtatcg
    23641 gttccccgcg cattcgccgt ggtgacggct atgtgcattc ccttgccgca gccaacgaac
    23701 aagaacactt cggcattcga aacgagctca ccaaccagtc gatgggcctc ctcgctgtgg
    23761 gtaccgagct ccaacatgtc accgaggatc acgaccaagg gggcattccg cttgcttgcg
    23821 atcattcgcg cggtgtccac tgcgatctcg accgatgctg gattggagtt gtacgtgtca
    23881 tcgatgacga ggcgctgagc ggttccggta cgaggctcca ttcggcctgg cgatgctgaa
    23941 acctgcgaga tcccgcgggc ggcatcttct agcggaagtt gcagagcgaa cgcaatcgcc
    24001 agggcgcccg cggcgttgag aactgcaccc tctccgagga gactcatctc gatctctagc
    24061 tcttgaccgc gaacctggag atgggcgcgc gtgcctccgg cgtggacatc ccaatcgatg
    24121 atgcgcacat cgttgcccgc ggtacgcccg taaaacagct tgcgcttggc ggccaccacc
    24181 gctgcgcgct tcttcaccgg aacgtcgtcg ccatatgcga tcgcaatgcc gtctcgcccc
    24241 agtgcctgta agagcgacac cttttcgtcg gctaccgctt caactgagcc gagaccctgt
    24301 gtgtgtgcga ggctggcccg ggtcacgatt cccacatcgg gagatgcgat ctcggcaaga
    24361 cgcgcgattt ctccgggctc gctggttccc atttcgacga ccgcggtgtc tacggtcgaa
    24421 tccaagcgaa agagagtcat cgggacaccg atgcgattgt tgaggttgcc gcctgtcttg
    24481 agcaccctgc gtccagccga ctccagggcc gccgcggtga ggtccttagt ggtcgtcttg
    24541 ccaaccgaac ccgtgattcc aatcagctgc ccgttccacg ccaagcggtg agcatgtgcg
    24601 agatctccga gcgcgcgcag cgtgtcgtcg accagcaccg cgctcaccgc gtcggggacg
    24661 gaggtgccgc gctcgaccag cacagccgtt gcaccggctt ctgcggctcg agctgcgaac
    24721 tggtgtgcat cgaagcgctc gccgcgaagc gcgacgaaca gattgccagg ctgaacggcc
    24781 ctcgagtcga tgaccacgcc agatacgctt tcccagtcgg gcccggtcgc gctgccaccg
    24841 gttgcggaga gcacctggtc tcggcgaaaa ccggctgtat tgatcggaat gggagtcgcc
    24901 atcagctcgg ccctttcagg ttcgcgatcg cggtacgcgc ctcttcgcga tcgtcgaagt
    24961 gggtgcgctc acttccgatg atctgatagg tttcgtgccc cttgccggcc aagagcacgg
    25021 tatccccgga ttcggctgcg ccaatggccg ctcggatcgc ttcctttcgg tcggccagga
    25081 cacagtaccc gcggcttgct ccgacgagat cgcgtggggc gattcgttgc ccgcccgcct
    25141 gcttcgcacc cacctccagc tcgtcgagga tgacctgcgg gtcttcgctt ctcggattgt
    25201 cgctcgtcaa aacgcagagg tcggcacctt ggatgcctac gcgccccatc tccgggcgct
    25261 tgccccggtc gcgatcaccg cccgctccga agaccacgat caagcggttc ggggtcactg
    25321 cccgcatggt ttgaagaacg cgatgcagcg catcggtcgt atgggcgtag tcgacgagta
    25381 cggcaacgtc gcgaggatgt gggatgcgct cgagccggcc aggacttccg ctggcggctt
    25441 cccacgcggc tgcggctgct ttcaagtcca ggcccagtgc atgcgcgcaa cccagtgcca
    25501 ccaaaatgtt ctcgagattg tgctcaccga gcatcggact tcggatcgaa atcgagcccg
    25561 caggtgtgtc tacctcggct cgaatcccag cgcgcgtaac ggaccattcg ctcgcgatga
    25621 tctcggcatt gctttcgggc cgtcgcgagc atcgcgtcac cgggacggat acgcgtt
//
LOCUS       DASRZF010000143         7349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4909447, whole genome shotgun sequence.
ACCESSION   DASRZF010000143 DASRZF010000000
VERSION     DASRZF010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 7349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7349
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4909447"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VFG22_06525"
     CDS             <1..266
                     /locus_tag="VFG22_06525"
                     /inference="COORDINATES: protein motif:HMM:NF016972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET6415932.1"
                     /translation="KDCAVRYLHDASGVQEKNLVYAGDSGNDRAAMLAGYRTIVVGNA
                     NSALKDDLARESAARGISERIYFAEHPYARGVLEGLRHFGLLP"
     gene            complement(356..3043)
                     /gene="polA"
                     /locus_tag="VFG22_06530"
     CDS             complement(356..3043)
                     /gene="polA"
                     /locus_tag="VFG22_06530"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HET6415933.1"
                     /translation="MATELPNKGDPKTIYVIDISSYVFRAYHALPPLSNSKGEPTHAV
                     TGVASMLLKLLREQEPRGVVVAMDSKGSSFRKDLFDAYKANRPPAPPDLEQQMERVRE
                     IAEAWGMSPMEAPGFEADDIIATLVTQARKKDLRVVIVSADKDLLQLIGPDVIMYDTM
                     RGKVFGAEETREKLGVDPEQVRDLLALMGDSSDNVPGVPSVGQKTAAKLLDAYGSLEG
                     IYANLEKITQKALKAKLDAHHEDALRSRTLVTLRHDVAIDEDAITRRFEGGDREALRA
                     LFGELELTRLLAQLEPAPSAQGRYETILSADDLGRIANLIRETGRFALYTVMDIDDPI
                     RAELVGVAVTSLEGMSVYIPLGHRYIGAPDQLSWEQAGPVLTPLLENSLIPKFTLAKR
                     ETVFWARQGVRLRGVRFDPSLASYLLDPARHAHRLEDVARQELDGSLRDEESVRGKGK
                     KALNWDEVEVAAVAGYANERADFTFRLSELLTPRMHEGEFERLFYEVELPLAGVLATM
                     ELNGIRVDTGLLQELSTAADSELERLHARCTELAGHEFNVSSPRQLETILFDELGLPV
                     LKKTKTARSTDQSVLEELAPMHDLPQAILDYRSVSKLKSTYLDALPREVNPETGRIHT
                     RYNQLVAATGRLSSSEPNLQNIPIRTEMGRRVRDAFVPEEGWSMLAADYSQIELRVLA
                     HLSRDPALVDAFSRGDDVHVQTACALFGVRPEEVTKHMRGQAKTVNFAVIYGQTEFAL
                     ARNLKIERGEAKRYIDAFFDQYAGVKSFLDEIVEQAVANGFVTTLLGRRRTLPDIRSR
                     NRNLRAGAERIARNTPIQGTAADIMKVAMVRIQHDMEKRRLQSRMVLTVHDELVFESP
                     QAERDVLQRLALDHMQNAVPLSVPLPVEAAWGPSWGAAH"
     gene            3155..4738
                     /gene="ffh"
                     /locus_tag="VFG22_06535"
     CDS             3155..4738
                     /gene="ffh"
                     /locus_tag="VFG22_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HET6415934.1"
                     /translation="MFETLTKGFRNARNRLAGVTELTEENIDQALRDVRLSLLEADVE
                     FGVTKAFLGRVKEKAIGQVVETAAKSKKRKVKVGPAEQFVKICQDELESMMAFDGEPV
                     EFAKRPKPTGIMMVGLQGSGKTTTSAKLARLLETSNARKPLLVAADVARPGAIEQLQV
                     LGEQIDVPVFSIPGGQPVEICKRGLAHAKKLKCDTIIYDTAGRLAIDEPLMKELDEIR
                     SSVDPQNVFLVVDAMIGQDSVKTAKSFHDLLGLSGVILTKLDGDARGGAAISIKNVTG
                     TAVKFVGTGETLDRLEEFRADGMASRILGMGDVVGLMKDFEDVVDEDKAEKDAKRMLR
                     GHFTLDDFLEQLQMLQSMGPLQDLLDKVPFFADSVPDGFQVDEREIGQIKAIVSSMTR
                     QERGKSELFQKQPTRLVRVAKGSGTSEKQVAEILQRFAFMKQMMGSIGSQAGMLSKIP
                     GMKQMAAANRLRDAVRTGGLEGNPMMANLAESLLEAAVAEQGGLGPGGSQAPRKIVDK
                     GKKKSKRKMQRKSRRKSRR"
     gene            4750..5595
                     /locus_tag="VFG22_06540"
     CDS             4750..5595
                     /locus_tag="VFG22_06540"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HET6415935.1"
                     /translation="MSVMHRALCLCLMFAGACTRDAPKAPPEQEGAAERRVEKTPRHD
                     LGGVPKIRVMHTKQPPNLDGSLDEPAWRLARSTGAFVETLKGGASKLQASAKLLWDEH
                     FLYLGAVVQDSLLRASQRHHDAHLWEQDSVELMIDPDGDELDYYEVQVSPGGVVFDTR
                     FDARRVPKPFGHMDWDGNVRVGVSTDGTLDDEERDTGYVVEIAIPWQAFSLEGKPVEA
                     PAVGDEWRANIYVLDLFEEGQRAAAWSPPGIGDFHVPPRFGILLFEGESDEMVNDLES
                     VGVPR"
     gene            5626..7302
                     /locus_tag="VFG22_06545"
     CDS             5626..7302
                     /locus_tag="VFG22_06545"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HET6415936.1"
                     /translation="MLRTRLIAGRTSGLILSAVFASIVTLASQVEVVLEPLRVDPLQP
                     APVTLRIPNDYLPKQLADRRAGLPQPLLVARGDTVSDPESQRIVRAFERERRPPEQEM
                     LLGIWISYFLMAYIFLAYLRLFTGGRGGLLRTQAGLLVLVGATCLGAKLVLLFTGLSP
                     FLLPLATVPLWAALYFDRRTATASGLVISLVCASFMHLSMPVVVVYLATTLGVVIFFH
                     DRKHATHMLVAGTAAGLFAALVLVSVAMSVGSGIDVIGDLARLNDSALLTTIAGGMLS
                     GVLAVLLQRLATATLGVVTRGRLQELTDVDQPLLRKMARDAPGSWQHARAMANLAEGA
                     AAAIGADALLTRVGAYYHDLGKTIQPKYFVENLVAGEPSPHGDLEPDVSADAIMAHVV
                     EGARILREGGVPEPVVEFAYTHHGTSVIEYFWHKCLEAGNPKGLSDAAFRYPGMRPRT
                     KETAILMLIDAIEAAARTVDEPSREKFEGVVQRVTNVKLRQGQLDECGLTMEDLRVLQ
                     STLTDTLCNAYHNRIKYPWQDREDEGETQLPVPGLATERDVALERSRDST"
BASE COUNT         1362 a         2266 c         2312 g         1409 t
ORIGIN      
        1 caaaggactg cgcggttcga tatttgcacg acgcctcggg ggtccaggaa aagaacctgg
       61 tttacgccgg agattcggga aatgatcgtg cagccatgct tgccggctac cgcaccatcg
      121 tagtcggcaa cgcgaactcc gccctcaagg acgatctcgc tcgcgagtct gcggccaggg
      181 gcatcagcga gcggatctac ttcgcagagc acccgtatgc gcgcggcgtg ttagaaggcc
      241 ttcggcactt tggactgtta ccttgaggat tcgaaacgca tgaaacgaat ttcgaccaaa
      301 ccggtctttg ccattgcgct acttgcgtcg ccctaggggt cgggcaccgg cgggcttagt
      361 gcgccgcgcc ccagctcgga ccccaggccg cttcgacggg gagcgggacc gacagtggca
      421 ctgcattctg catgtggtcg agcgcaagcc tttgcagcac gtctcgttcg gcttgcggcg
      481 actcgaagac caactcgtcg tgcacggtca gcaccatgcg gctctgaagc cgacgctttt
      541 ccatgtcgtg ctgtatgcgc accattgcga ccttcatgat atcggcggcc gtgccctgga
      601 tgggggtatt gcgcgcgatt cgctcggcac cggcgcgaag gttgcggttg cggctgcgta
      661 tgtcggggag tgttctgcgc cgacctagca aggtggtgac gaagccgttg gcgaccgcct
      721 gttccacgat ctcgtcgagg aacgacttga ccccggcgta ctggtcgaag aacgcatcga
      781 tgtagcgctt ggcttcgcca cgttcgatct tgaggttgcg cgccagggca aactccgtct
      841 gtccgtagat gacggcgaag ttcaccgtct tggcttggcc tcgcatgtgc ttggtcacct
      901 cttcgggtcg aaccccaaac aaggcgcagg cggtctggac gtgcacatcg tcgcctcgag
      961 aaaacgcatc gacgagtgcg ggatcgcggc tgaggtgggc gagcacccga agctcgattt
     1021 gcgaatagtc cgccgcaagc atcgaccagc cttcttcggg cacgaacgcg tcgcgaacgc
     1081 ggcgacccat ctcggtccgg atggggatgt tctggagatt cggctcactc gaggagagcc
     1141 tgcctgtcgc cgcgaccagc tggttgtagc gcgtgtggat gcgtccggtc tccggattaa
     1201 cctctcgcgg taaagcgtct aaatacgtgc tcttgagctt ggaaaccgag cggtagtcta
     1261 ggattgcctg cggcagatcg tgcatcgggg cgagctcttc caaaacgctc tgatcggtgg
     1321 atcgagcggt tttcgtcttc ttcaggacag gcaagccgag ctcatcgaag agaatggtct
     1381 cgagctgcct cggagaggag acgttgaact cgtgtcccgc gagctcggtg catcgcgcat
     1441 gcagcctctc gagctcgctg tccgccgcag tcgaaagctc ctggagcagc cctgtgtcga
     1501 cgcgaatccc gttgagctcc atggtcgcca ggaccccagc gagggggagc tctacttcgt
     1561 aaaagagccg ctcgaactcc ccctcgtgca tgcgaggggt gagcagctca ctcaggcgaa
     1621 aggtgaaatc cgcgcgctcg ttggcatatc ccgcgactgc ggcgacctcg acctcgtccc
     1681 agttcagcgc cttcttgccc ttcccacgga cggattcctc gtcgcgtagc gatccgtcga
     1741 gctcctgtcg ggccacgtcc tcgaggcgat gtgcatgtcg cgcagggtcg agaaggtagc
     1801 tggcgagcga cgggtcgaag cgtactcccc gaagcctgac gccctgccgc gcccagaata
     1861 cagtctcccg cttcgcgagc gtgaacttgg ggatcagaga gttttcgagc agcggggtca
     1921 gcacggggcc cgcttgctcc cagctcaact ggtcgggagc gccgatgtag cggtgcccga
     1981 gtgggatgta gacgctcatc ccttccaagc tggtcaccgc gactccgact agctcggctc
     2041 ggattggatc atcgatgtcc atcaccgtat acagcgcaaa acggcccgtc tcgcggatga
     2101 ggttggcgat tcggccaagg tcatccgcgc tcaggatcgt ttcgtatcgc ccctgggcgc
     2161 tcggcgctgg ctcgagctgg gcgagcaagc gagtgagctc gagctcaccg aagagcgcac
     2221 gaagggcctc cctatccccg ccttcgaatc tgcgcgtgat cgcatcttcg tcgattgcga
     2281 catcgtgtcg cagggtcacg agcgttcgtg acctcagggc gtcctcgtgg tgtgcatcga
     2341 gcttggcttt gagtgccttt tgcgtgatct tctcgaggtt cgcgtagatg ccctccaagc
     2401 tgccgtatgc gtcgagcagc ttagcagccg tcttctgtcc gaccgacggc acgcccggca
     2461 cgttgtcgga gctgtcaccc atgagtgcga gcaagtctcg cacctgctca ggatccaccc
     2521 cgagcttctc acgggtctcc tcggccccaa acaccttgcc gcgcatggtg tcgtacatga
     2581 tcacgtccgg accgatcagc tgcagcaggt ccttgtctgc gctcacgatc acgacgcgca
     2641 ggtccttctt tcgggcctgt gtcaccaagg tggcgatgat gtcgtcggcc tcgaacccgg
     2701 gggcctccat cggcgacata ccccaggctt ctgcgatctc ccggactcgc tccatctgct
     2761 gctccagatc gggcggtgcg ggcggacggt tcgccttgta ggcatcgaac aggtctttgc
     2821 gaaacgagct tcctttcgag tccatcgcga cgacgactcc tcgcggctcc tgctcacgaa
     2881 ggagcttcag cagcatgctt gcaaccccgg ttaccgcgtg ggtgggctcc cctttgctgt
     2941 tcgagagtgg tggaagcgcg tgatacgcac gaaacacata ggagctgatg tcgatcacgt
     3001 agatcgtctt tggatccccc ttattgggta gctccgtcgc catgcggtgg caccatccaa
     3061 cgctcgaatc cggaggtcaa ggagcgaagc aggttgcgtg cccgcttccg tgcatgctac
     3121 gcgcacgagc cgcagccccg aaaaccaccc ccgcatgttt gagacgctca caaagggctt
     3181 tcgcaacgcc cgcaaccgcc tcgctggagt cacagagctc accgaggaga atatcgatca
     3241 agcgcttcga gacgtgcgct tgtccttgct cgaggcggac gttgagttcg gcgtcaccaa
     3301 ggcatttctc ggacgcgtga aagagaaggc catcggccag gtcgtcgaga ccgccgccaa
     3361 gtcgaagaag cgcaaagtca aggtcgggcc tgccgagcag ttcgtgaaga tctgccagga
     3421 tgagctcgag tcgatgatgg cgttcgacgg cgagcccgtg gagtttgcca agagaccaaa
     3481 gccgaccggc atcatgatgg ttggccttca aggctcgggt aagaccacga cctcggccaa
     3541 gttggcccga ctgctcgaaa cgagcaacgc gagaaagccc ctcttggttg ccgccgacgt
     3601 tgccagacca ggcgccatcg aacagcttca agtgctgggc gagcagatcg acgttcccgt
     3661 tttttcgatc ccgggtggac agccggtgga gatctgtaag cgcgggctcg cgcacgcaaa
     3721 aaagctgaag tgcgacacga tcatctacga caccgccggc cgcctcgcga tcgacgagcc
     3781 cctcatgaag gagctcgacg aaattcggag ctccgtcgat cctcagaacg tgttcctcgt
     3841 ggtcgatgcc atgattggcc aggattcggt caagaccgcc aagtcttttc acgatctgct
     3901 cggtctgagc ggcgtgattc tcaccaagct cgacggtgat gcgcgcggcg gcgcggcgat
     3961 ttcgataaag aacgtgaccg ggacagcggt gaagttcgta ggcacgggcg agaccctcga
     4021 tcggctcgaa gagttccggg cggacggcat ggcgagccgc atcctgggca tgggtgacgt
     4081 cgtcggtctg atgaaggact tcgaggacgt cgtcgacgaa gacaaggcgg agaaggacgc
     4141 gaagcgaatg ctgcgcggcc atttcacgct cgatgatttc ctagagcagc tgcagatgtt
     4201 gcagagcatg ggtccgctcc aggacctgct cgacaaggtt ccgttcttcg ccgatagcgt
     4261 gccggacggc ttccaagtgg acgaacgaga gatcggccag atcaaagcca tcgtgagctc
     4321 gatgactcgt caagagcggg ggaaatccga gctctttcaa aagcagccca ctcgcctggt
     4381 gcgagtcgcc aaaggctccg gcacgagcga aaagcaggtc gcagagattc tgcaacgttt
     4441 cgcattcatg aagcagatga tgggcagcat cgggtcgcag gcgggcatgc tctcgaagat
     4501 cccaggcatg aaacagatgg ccgcggcgaa tcggcttcgt gatgcggtgc gaaccggggg
     4561 cctcgaaggc aatccgatga tggcgaacct cgctgaatcc ttgctcgagg ctgcggtggc
     4621 ggagcaaggc ggactgggcc cgggaggtag ccaggccccg cgtaagatcg tcgacaaggg
     4681 caagaagaag tccaagcgaa agatgcagag gaaatcgcgc cgcaagtcgc gccgatgaat
     4741 gcgccatcga tgagcgtcat gcaccgcgct ttgtgcctct gcctgatgtt tgccggcgcc
     4801 tgcacacgcg atgcacccaa agcgccaccg gagcaagaag gcgcagcgga gcggcgcgtg
     4861 gagaagactc cgagacacga cttgggtggc gtccccaaga tcagggtgat gcacacgaag
     4921 cagcctccta atctcgacgg ctcgctcgac gagccggctt ggcggcttgc gcggtccacc
     4981 ggcgcgttcg tcgaaactct caaaggcggc gcgtccaagc tgcaggcttc cgccaagctg
     5041 ctttgggacg aacattttct ctacctcggc gccgtagtgc aggactccct gctccgagcg
     5101 agtcaacggc atcacgatgc gcacctctgg gagcaagact cggtcgagct catgatcgac
     5161 cccgacggcg acgagctcga ctactacgag gtccaagtct cgccaggcgg cgtggtgttc
     5221 gacacgcgat tcgatgcgcg ccgggttccc aagcccttcg gtcacatgga ttgggacggc
     5281 aacgtgcgag ttggggtctc gaccgacggc acgctcgatg acgaggaacg cgacactggc
     5341 tacgtcgtcg agatcgcgat cccgtggcag gcgttcagtc tcgaaggcaa acctgtcgaa
     5401 gcacccgccg ttggtgatga atggcgtgcg aacatctacg tgctggactt attcgaggag
     5461 gggcagcggg cagccgcctg gtcgccgcca ggaattggag atttccatgt gccgccccga
     5521 ttcggtatcc tgctcttcga gggggagtcc gacgagatgg tcaacgacct tgaatctgtc
     5581 ggggtgcctc ggtagggtgc cgccctccgg gacggttcaa ccaggatgct tcgcacacgc
     5641 ctcatcgccg gtcgcacctc gggcttgatc ctcagcgccg tcttcgcttc gatcgtcacg
     5701 ctcgctagcc aggtcgaggt cgttctggag ccacttcgcg tcgatccgct acagcccgcg
     5761 ccagtgaccc tgcggattcc gaacgactac ctgcccaagc aactcgcgga tcgtcgtgct
     5821 ggcctgcctc agccgctgct ggtggcgcgc ggcgatacgg tcagcgaccc cgagagtcaa
     5881 cgtatcgtcc gcgcgttcga aagggagcgg cgcccccccg aacaggagat gttgctcggg
     5941 atctggatca gctacttcct gatggcgtac atcttcctcg cctatctccg cctattcacg
     6001 ggcggccggg gcggactcct gcggacccag gccgggctcc tcgtcctcgt gggggccacc
     6061 tgcctcggag ccaagctcgt gctcttgttc acggggcttt ctccgttcct gctgccactg
     6121 gccaccgtgc cattgtgggc cgccctctac ttcgaccgga ggacggccac ggcttcgggt
     6181 ctggtgatct cgctggtctg cgcttcgttc atgcatctct cgatgccggt ggtggtcgtc
     6241 tacctcgcca cgaccctagg cgtcgtgatt ttcttccacg accgcaagca cgccacccac
     6301 atgctcgtcg ccggcaccgc cgccggtctc tttgccgcgt tggtgctggt tagcgttgcg
     6361 atgtccgtcg gcagtgggat tgacgtcatc ggcgacctgg cccgactgaa cgactccgcc
     6421 cttctgacga cgattgccgg tggtatgctt tcgggcgtcc tggcggttct cctgcagcga
     6481 ctcgctacgg ccactcttgg cgtcgttaca cgtgggcggc tccaggagct taccgatgta
     6541 gaccaacctc ttctgagaaa gatggcgcgc gatgcaccag gctcgtggca gcatgcccgc
     6601 gcgatggcca acttagccga aggcgcggcc gctgcgatcg gtgcggatgc cctgctcacg
     6661 cgcgtcggcg cgtattacca cgacctcggc aagacgattc agccgaagta cttcgtggaa
     6721 aacctggttg ccggcgaacc ttctccgcat ggagatctgg agcccgacgt gagcgcagac
     6781 gcgatcatgg cacacgtggt tgaaggtgcg cggatcctac gcgaaggagg cgttccagag
     6841 ccggtcgtcg aatttgccta tactcatcac ggcacgagcg taatcgaata cttctggcac
     6901 aagtgtttgg aggccgggaa ccccaaggga ctttccgatg cggccttccg ttatccaggg
     6961 atgcggccca ggaccaagga aaccgcgatc ctcatgctca tcgacgcaat agaagcagcc
     7021 gcccgcaccg tggatgagcc gagccgtgag aaattcgaag gtgtcgtgca acgcgtgacc
     7081 aacgtcaagc tccggcaagg tcagctcgac gaatgcggcc taaccatgga ggacttgcgc
     7141 gtattgcaga gcacgctgac ggacacgctt tgcaacgcgt accacaaccg gatcaagtat
     7201 ccatggcaag acagggagga cgaaggcgaa actcagctgc ccgttccagg cctcgccacc
     7261 gaacgagacg tggcactaga gcggagccgt gactcgactt agcggtgcct ggctcgtcgg
     7321 cgccgcttgc ttgatcgggg cttgcgatc
//
LOCUS       DASRZF010000144        13204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3189629, whole genome shotgun sequence.
ACCESSION   DASRZF010000144 DASRZF010000000
VERSION     DASRZF010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 13204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13204
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3189629"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VFG22_06550"
     CDS             <1..482
                     /locus_tag="VFG22_06550"
                     /inference="COORDINATES: protein motif:HMM:NF014886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HET6415937.1"
                     /translation="AGRYPVQAVSMMDAIIREVESGYVEDLAREFRVLRNLGDEDWSF
                     ANAAARAAAELTIHLPLKAVVVFTQDGRSAGLLAEHRPRAPILAITSNVQVARRLALE
                     WGVIPKLEVPPESLDETLRIATSLLVQEKLCRPGEAFAMVVGWPVSGRTNTLKLHRL"
     gene            515..1753
                     /locus_tag="VFG22_06555"
     CDS             515..1753
                     /locus_tag="VFG22_06555"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6415938.1"
                     /translation="MEELPEQIGRYRIERLLGSGSMGNVYLGHDSDLDRPVAIKTLRD
                     LVLDAEMREVFLRRFQNEARAAARLQHPNIVQIFDVGEDPKLGPYLVFEYVKGHTLKQ
                     VLKKEGPLSRARLLDLAQCIADALATAHLAGVIHRDIKPENLLITENGQVKLADFGIA
                     RVPNADLTKEGQFLGTPCYSAPETLRTGEYSEQSDLFSFGAVIYEAVSGERAFPGMEA
                     MAVAHKVMSSDPVPPSEANRGARVPSKVDAAILRALRKNPSERYASARTFVSALREAY
                     DGRTPPRARRKRMGLPLSIVLGLAALVAVWIGIQTYDSARIGAVTDADAGTDSGADTD
                     SGTGTDAGTDAGTGTATLSPHEREEAAKDELATARDALERGDLDAAASALERAARFDP
                     SNPDIEALRIQVGAATPDGG"
     gene            complement(1750..2025)
                     /locus_tag="VFG22_06560"
     CDS             complement(1750..2025)
                     /locus_tag="VFG22_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415939.1"
                     /translation="MRKIVIQMAAVMALSMGCGHESAAMQQIRSAAAVDLDCDASMIE
                     FVEDKPMSKRVSGCGRTLTYMSKCNATASGGQSCQWKAVRDDSNKLN"
     gene            complement(2075..2788)
                     /locus_tag="VFG22_06565"
     CDS             complement(2075..2788)
                     /locus_tag="VFG22_06565"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HET6415940.1"
                     /translation="MSALVQVSHALSRAVSELEFGGPVTHVYNPLEYAKRSHEIYLGR
                     FGEGSKEVVLVGMNPGPFGMGQTGVPFGDVGFVRDWMGIEAPVDHPAREHPKRPVQGF
                     ACERSEVSGSRLWGWAKTRFGNPEQFFARFFVINYCPLLFIESSGRNRTPDKLPASER
                     AGLLSACDQALRSAIDVLEPKLVIGVGAWATKRVSAALGDDAPRIGTVLHPSPASPKA
                     NRGWAPQAESDLRALGVEL"
     gene            complement(2795..3703)
                     /gene="cysK"
                     /locus_tag="VFG22_06570"
     CDS             complement(2795..3703)
                     /gene="cysK"
                     /locus_tag="VFG22_06570"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HET6415941.1"
                     /translation="MTDRIYDGALDLVGHTPLVRLTRITGDKGAEICGKLESSNVGGS
                     VKDRPALAMILAAEASGELGPGATVVEATSGNTGISLAMICAVRGYRCVIVMPEDMSV
                     ARRHLLKAYGAEVVLTGAQDGMAGAVEQAERLLKTTPGSMMCGQFENPANPDIHAKTT
                     AEEIWEATSGRVDAFVAGVGTGGTLTGVGRVLKQRNPNVRVIAVEPRASAVLSGGKPG
                     LHGIQGLGAGFIPAVLDTELIDEVITVTDLAADRMTKRLAREEGLLLGPSAGANVHAG
                     LEVSRRMEVGQRVVTILCDGGERYLC"
     gene            complement(3730..4662)
                     /locus_tag="VFG22_06575"
     CDS             complement(3730..4662)
                     /locus_tag="VFG22_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET6415942.1"
                     /translation="MLLKLTGTGGSNLRALEAELGVSMGLRGSTIHLQGPNREVELVE
                     RVLHELIDALQFRDLSPVEMARAARTLRAHPQMRLRALLDDVVLTTSSRRIVSPKGVT
                     QQSYIQAIRSHDIVFGIGPAGTGKTYLAMAMAIRALQERQVKRIIMTRPAVEAGERLG
                     FLPGDLAEKINPYLRPLYDALHDMMEPGRIAALIERGTIEVAPLAFMRGRTLNDSFVV
                     LDEAQNTTPEQMKMFLTRLGFDSKTVITGDVTQIDLPGGTRSGLRHAERVLSKIKGIE
                     FIYFSHTDVVRHPLVQQIVLAYESHESGSSDGDI"
     gene            complement(4781..6817)
                     /locus_tag="VFG22_06580"
     CDS             complement(4781..6817)
                     /locus_tag="VFG22_06580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6415943.1"
                     /translation="MPPTVLLLDDDTQFRSSVTPALEALGLRVLNAAKGSIARAWLEE
                     EEPSLLVVDGLLPDTNGITWIEELRRDGFETPIIFVSAFYRDLTTFKHLTGDLGVLKV
                     FHKPVTVDRFAREVSSAISAPHSLAPVVTRLLDEPEDPIVFPDEASEPGVDAEKAKQA
                     YTSLLPIAADNLTGAIRRVHSEGDRTSVLAEALRQAHDLHGAAQTHGFGEISDAVGRI
                     EAELRQLQESGRLDWASMFESIDAVRAHAATAVGRPVDIRLTAAAPPSVTPAASFFLH
                     RPIPQDFVPTVVVLEDDPAMIAYLRSALDEVLVHLRPVSTVEEALKISGRNPPTAVLV
                     GWPLEEQDALSRFLPMLRSLHGCSDTPVIVLSLDDDPHTRALVAQLGADIFLPQPIEL
                     VRLHHAIDTAVERAMTPRPRVAVLRDPDAAREIQDAGIECLLYFSFDELLKEIDVQCP
                     DVILLGSSVTGKQVPAIIRMSAWDADCSLLCFDDSPLADELGITERLDPVSGWLVELH
                     REAERMARLRRQQLRCSQTGLYARPAAVAGLEAGLSSAQRNGRTYSIGLIRATGLDGL
                     DHAKARRLRAHLGRLIHGRFRREDVRGRWDEHTFVVGFEGSSTRAVVEVVRRLKDELD
                     AQRTRQAEELIFLHMAVGLSSYPLDGDTSRALILAAHERLETAAKRDPDALVWR"
     gene            complement(6874..7581)
                     /locus_tag="VFG22_06585"
     CDS             complement(6874..7581)
                     /locus_tag="VFG22_06585"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6415944.1"
                     /translation="MAPWAQQILDAANEHYERADYPLAQPYYEKLVQQGLRYPDVFNR
                     LGVIYHLGGRLPEARWCFQQALELNGGYMEAALNLTVTCNELGEYGSGVRTLERAQRH
                     SDSSNDAYRRGKVANLHRSLARAYLEIDRPEDAMHEFRRALRLCPKFHDIRLELAKLL
                     RLRGNAQGAREELTLILQLEPAHHDARALLGVIEIETGDRKEGLQHLRAVVEQCAEHP
                     VARAYLKQANSDFRRAS"
     gene            complement(7637..8437)
                     /locus_tag="VFG22_06590"
     CDS             complement(7637..8437)
                     /locus_tag="VFG22_06590"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HET6415945.1"
                     /translation="MGLRLYRFVFCMLLASSMACGNSSMSKKSSTRKLLDPDDDARSG
                     YEKALLNFRRGDCLAAEPMFREVRREFPYSRFAALAELRIGDCQFKNEAYPEAIQTYR
                     QFVRIRPSHKEIPYARFRIAEAYYNQIPGGWFMTPPAAERDQSAARDALIQLRRFVVD
                     YPDDQRVPKANELMEKCMSLLAAHELYVARFYLKRDAYRGVISRLNGLLTAYPGSGVE
                     REALLLLGEVYLEAKEVEAAKETFNQIIQRFPDSAEAKKARSMLGKLG"
     gene            complement(8505..9164)
                     /locus_tag="VFG22_06595"
     CDS             complement(8505..9164)
                     /locus_tag="VFG22_06595"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HET6415946.1"
                     /translation="MRKTARTLLISDVPEFGLASFQIWWQSSLVSASLAPGFLVAAPS
                     MQCPFFNHTLVLLIDHGNEGSFGFVVNRMTELGIADVFNEVGVTGPAAASVCAPVMLG
                     GPVSPESGWLLYDPEGVPQASSGQAIAVGPRIACSASIELLARMARGDGPETCAMILG
                     YSGWGAGQLESEMKQGSWIPVDLDSALVFDTPIENRWEAALATLGIDPARLIDNQIAS
                     A"
     gene            9136..9885
                     /locus_tag="VFG22_06600"
     CDS             9136..9885
                     /locus_tag="VFG22_06600"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET6415947.1"
                     /translation="MSKVRAVLRTGAFVGFSAGILAAYEASRALTPASEQENLADRYR
                     ERLTRGLLRVFGAKLLIEGHYSHRGGALVVSNHQSALDIGVMLSLFRGVLVSRHDVAD
                     WPLLGRLAKHGDTIFVDRDDRRSGAAALREIRRRVKEGRTVVAFPEGGTFPGDSVQEF
                     HPGAFAAVRSLGVPIIPVGLAYAPSVPYSGESFGRHIARVAARPMTRIAVHLGEPLPG
                     DLDARAMAEAARSRVESLVSDARRALDGVPA"
     gene            9989..11623
                     /locus_tag="VFG22_06605"
     CDS             9989..11623
                     /locus_tag="VFG22_06605"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6415948.1"
                     /translation="MVRLIQQSKETAPDRDNLGWELLPSALWDLDFQRPLEWIEARGC
                     RDANEFKDLVRNDPNALAECLDRIRIHRVSAGAVALVGAASEEECIRRSLELLTAESK
                     ADFLRVLIAAFRKEHDVHIETAIMTCSREPRDVRVHWRFVGGEEGRFQRAIVSVVDMT
                     AERDLQQRLLLAERMSAIGTLTASIIHEIKNPLTFVWNHLRSLRDSGERLSPEGRELL
                     EEAYEGTERIRLISNEITSLSHGADGVEVETVNLKEVLDAALRIAGPEIQHRATVVRE
                     YEEGSLYIRGSRTRVGQVFLNLIVNAAQAIDPGDLAQNAITVRARSTERDRVCIDIQD
                     TGPGISPQLLRRIFDPFVTTKPAGRGTGLGLSITRRIVHSLEGTIEIQSHPGQGTVAR
                     VVLPRASRPQRPLTVPPPSISAIRRAARGKLSILVVDDEPVIARLIQKALVDHDVTTA
                     HDGREAVALMGEHAYDVILCDLIMPEMTGMDVYRAALQRAKPMNDRIVFMTGGAFTQR
                     ARDFLESVPNLRIEKPFELTHLERTIYEAADIIDPH"
     gene            11630..13012
                     /locus_tag="VFG22_06610"
     CDS             11630..13012
                     /locus_tag="VFG22_06610"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET6415949.1"
                     /translation="MSRPKMIVIGLDCAAPSLVFDRYQDAMPNLGALMERGTWGPLRS
                     SEPPITVPAWTCMLSGRDPGELGLYGFRNRVEGETRLCLADGKDVRVKRVWDWLGEHG
                     YRVAPLFVPLTYPPAPVRGRMVSGFMWPGGEAPWCFPRGLEAELVERFGPYQADVEGF
                     RASDLDRIYRDIVAMTEQHLSIAEHIWDEEKPDFMMMVEIGLDRFHHAFWRHIDPAHP
                     GYDPASPWKDLGLEYYGRLDQNIGRLLARTDEGTSVLVVSDHGARAMHGGFCINEWLR
                     RNGYLSLMDVPNEPSALDHARVDWSRTRAWAEGGYYARVFLNVEGREPKGIVAPERIR
                     SLRTQLREELENVTDDEGNAIAVSVRVPEEDYREARGFPPDLMVYFDDLRLRAIGSVG
                     TGRIRVAENDTGPDGCNHDWSGIFVMSGGGTLARGYVDGAEIYDIAPTILGAFGIPRP
                     PELFGRDWTK"
BASE COUNT         2520 a         4121 c         4152 g         2411 t
ORIGIN      
        1 cagccggacg gtaccctgta caagccgtct ccatgatgga cgcgatcatt cgagaagtgg
       61 agtcgggcta cgtagaagat ctcgcgcgcg agttccgggt tctgcgaaac ctgggcgatg
      121 aagactggtc ttttgcaaac gccgctgcgc gtgccgccgc cgagctcacg atacatctgc
      181 cgctcaaggc cgtagtcgtt ttcacgcagg atggtcgctc ggcaggcttg ctcgccgaac
      241 atcggccgcg cgcaccgata ttggcgatca ccagcaacgt gcaagtagca cgccgcctcg
      301 cgctagaatg gggcgtcatc ccgaaactcg aggttccccc cgagtcactc gacgagacgc
      361 tgcgcatcgc cacgtcgctg ctcgtgcagg agaagctatg tcgacctgga gaggcgttcg
      421 cgatggtggt cggatggccc gtttcaggtc gcaccaatac gctcaagctg caccgtctct
      481 agcctcctgc tctcctatgc actagatcgc ggagatggaa gaactgccgg aacagatcgg
      541 ccgctatcgg atcgagcgac ttctcggctc gggctcgatg ggcaatgtct atctggggca
      601 cgattcagat ctcgatcggc ccgtggcgat caagacgctc cgcgatctcg tgctcgacgc
      661 ggagatgcgt gaagtgtttc tccggcgctt tcaaaacgag gctcgtgcag ccgcacgcct
      721 gcaacacccc aacatcgttc aaatattcga cgtgggtgag gatcccaagc tcggccccta
      781 tttggttttc gagtacgtga agggacacac actcaagcag gtgctcaaga aggagggccc
      841 gctgtcgcgc gcaaggctgc tcgacctcgc acagtgcatc gcagatgccc tcgcgacggc
      901 acatctggcc ggggtcattc atcgagacat caagccagag aatctgctca tcaccgagaa
      961 cggtcaggtg aagttggcag actttggcat cgcccgcgtt cccaatgcgg atctcaccaa
     1021 ggaaggtcaa tttctaggga caccctgcta ctcggctcca gagaccttgc gcaccggcga
     1081 gtacagcgag caaagcgatt tgttctcgtt tggggccgtc atttacgaag ctgtttctgg
     1141 cgaacgcgcg ttcccgggca tggaggccat ggcggtcgcg cacaaggtga tgagcagcga
     1201 cccggttccc ccgagcgaag ccaatcgggg cgcaagggtc ccgagcaagg tcgacgctgc
     1261 aatcctgcgc gcacttagaa agaacccttc ggagcgttac gcgagcgcgc gcacattcgt
     1321 ctctgcgctc cgggaagcgt acgacggacg aacgccgccc agagcaagac gcaagcgaat
     1381 ggggttgcca ttgtcgatcg tcttgggact cgctgctctc gtcgcggtgt ggatcggcat
     1441 ccagacttac gattccgcgc gaatcggcgc tgtcactgac gccgacgctg gcactgactc
     1501 tggcgctgac actgactctg gcactggcac tgacgctggc accgacgctg gcactggcac
     1561 cgcgacgctc tccccacacg agcgagagga agctgccaag gatgagctgg cgactgcgcg
     1621 cgacgcactc gaacgcggcg acctcgacgc cgccgcgagc gcactcgaac gcgcggcgcg
     1681 cttcgacccg agcaatccag atatcgaggc gctccgcatc caagtagggg ctgctacgcc
     1741 cgacggcggc tagttgagct tgttgctatc gtcgcggact gctttccatt ggcagctctg
     1801 cccgccgctt gccgtcgcat tgcatttgct catgtaggtg agcgtccgcc cacagccgct
     1861 cacgcgctta gacatcggct tgtcttccac gaattcaatc atggatgcat cacagtcgag
     1921 atcgaccgcg gctgcgctgc gtatctgttg catggctgcg gactcgtgac cgcagcccat
     1981 gctgagcgcc atgacggccg ccatctggat gacaattttc cgcatcgctc gatcccccac
     2041 gccaagcata ccgcatgcat gcgcgctact gcgcttataa ctcgacgccc aaggcgcgca
     2101 aatcgctttc cgcttgcgga gcccagcctc gattggcttt ggggctggcc ggactcggat
     2161 gaaggacggt tccgattctc ggcgcatcgt cgccgagcgc ggcgctcacc cgcttggtcg
     2221 cccaggctcc tacgccgatc acgagcttgg gttcgagcac gtcgattgcc gagcgcaacg
     2281 cttgatcgca ggcactcaac aggcctgcac gctcgctagc cgggagcttg tctggcgttc
     2341 ggtttcgtcc actcgattcg atgaacagaa gcgggcaata gttgatgacg aaaaaccttg
     2401 caaagaattg ctccgggttg ccgaaccggg tcttggccca tccccaaaga cgagagccgc
     2461 tgacttcgga ccgctcacac gcgaagccct gtaccggtcg tttcgggtgc tctcgggccg
     2521 ggtggtcgac cggtgcttcg atgcccatcc aatcgcgaac gaacccaaca tcaccgaacg
     2581 gcacgccggt ctgccccatt ccgaatggtc ccgggttcat cccaaccagg actacctctt
     2641 tgctgccctc tccgaatcta ccgaggtaga tctcgtggga gcgtttcgcg tactcgagcg
     2701 ggttgtacac gtgggtcacc ggcccgccga actcgagctc ggatacggcg cggctcaatg
     2761 cgtgggatac ttgaacgaga gcgctcatcg cagactagca gaggtatcgc tctccaccgt
     2821 cacagagaat cgtcacgact cgttgtccga cttccattcg gcgagacact tcgagcccgg
     2881 cgtgcacgtt cgcacctgcg gacggtccga gcagcaatcc ctcctcgcgg gcaagccgct
     2941 tggtcatgcg gtccgctgcg agatcggtca cggtgatcac ctcgtcgatc agctcggtgt
     3001 cgagcacggc gggaatgaaa ccagcgccca gaccctgaat tccgtgaagg ccaggcttgc
     3061 cccctgagag cacggcgctg gctcgtggct cgacggcgat cacgcgaacg tttggatttc
     3121 gctgcttgag cacgcgcccg accccggtga gagtgcctcc cgtgccgacg cctgcaacga
     3181 acgcatcgac tcggccggag gtggcttccc agatctcctc ggcggtcgtc ttcgcgtgaa
     3241 tgtcggggtt cgctgggttc tcgaactgcc cgcacatcat cgagccaggc gtggtcttca
     3301 agaggcgctc cgcctgctcc accgcgcccg ccatcccatc ctgagcgccg gtcaaaacga
     3361 cctctgcacc gtacgccttc agcaggtggc gacgggcgac gctcatgtct tcgggcatca
     3421 cgatgacgca tcggtagcct cgaactgcgc agatcatcgc aaggctgatc ccggtgtttc
     3481 cgctggtggc ttcgacgacc gtagcgccag ggccgagctc ccccgaagct tcagcagcga
     3541 ggatcatcgc cagggctggg cggtccttga ccgaaccccc tacgttgctc gattcgagct
     3601 ttccgcagat ctcggctccc ttgtcgcccg tgatccgcgt cagccgtacc aaaggcgtgt
     3661 ggccaacgag atccagcgca ccatcataga ttcgatccgt catgtggcgg ctagcatacc
     3721 aggcggggct tagatgtcac cgtcggaaga gccagattcg tgggactcgt acgccaggac
     3781 gatctgctga accaaggggt gcctcaccac gtcggtgtgc gaaaagtaga tgaactcgat
     3841 gcccttgatc ttggaaagca ccctctccgc atggcgaagc ccactgcgag tgccgcctgg
     3901 cagatcgatc tgagtcacgt caccggtaat gacggtcttc gagtcgaagc cgaggcgggt
     3961 cagaaacatc ttcatctgct ctggcgtggt gttctgcgcc tcgtcgagca ccacgaagct
     4021 gtcgttcaag gttcgccctc gcatgaaggc caggggagcg acctcgatcg ttccccgctc
     4081 gatcagagct gcgattcggc caggctccat catatcgtgc aaggcatcgt agagcggacg
     4141 aagatacggg ttgatcttct ctgccaagtc gccaggcaag aacccgagac gctcgcccgc
     4201 ttccacagcc ggccgcgtca tgatgatgcg cttgacttgc cgctcctgga gggcacgaat
     4261 cgccatggcc atcgccaggt aggtcttgcc cgttcccgcc ggcccgattc cgaagacgat
     4321 gtcgtggctg cggatcgcct ggatatagct ctgctgagtg acccccttcg gtgaaacgat
     4381 gcgacggctg gaggtcgtca agacgacgtc gtccaagagc gcgcgcagcc tcatctgcgg
     4441 atgtgcgcgg agcgtgcgag ccgcccgtgc catctcgacc gggcttagat cacgaaattg
     4501 aagggcgtcg atgagctcat ggagaacgcg ttcgactagc tctacctcgc gattgggtcc
     4561 ttggaggtga atcgtcgacc cgcggaggcc catcgacacg ccgagctccg cttctagagc
     4621 acgcagattc gatccgccgg tgccggtgag cttgagcaac aagcctggat ctgcgacctc
     4681 gatgtcggcg gctacatcga cctctgctgg ccgggatggc gcggtggttt cgatggccga
     4741 agtttaccct gtcctgcgcc gggaggcgag agccactcga ctagcgccac accagcgcgt
     4801 cagggtcccg cttggccgcg gtttcgaggc gctcgtgggc ggcaagaatc aatgcgcggc
     4861 tggtgtctcc gtcgagcgga taggaggaaa gccccactgc catgtgaagg aagatcagct
     4921 cttcggcttg cctggtccgc tgtgcatcga gctcgtcctt gagcctgcga acgacctcga
     4981 cgacggcccg cgtgctcgat ccttcaaacc cgacgacgaa cgtatgctcg tcccagcgtc
     5041 cgcgaacgtc ttcacggcga aacctgccgt ggatgaggcg acccaagtga gcgcgtagcc
     5101 gtcgcgcctt ggcgtggtcg agtccgtcga gccccgtcgc gcgaatcaag ccaatcgaat
     5161 acgtgcgccc attgcgctgg gccgaggaga gaccggcctc tagcccagca accgcggcag
     5221 gccgcgcata caagccggtc tggctgcacc gcagctgttg tcggcgaagc cgcgccatcc
     5281 gttcggcttc gcggtggagc tcgaccagcc agcccgatac ggggtcgagg cgttcagtga
     5341 tgccaagctc atcggcgagc ggcgaatcat cgaagcagag caggctacag tccgcatccc
     5401 aagcagacat gcggatgatg gcgggcacct gtttgccggt gacactcgag cccaggagaa
     5461 tgacgtcagg gcactgcacg tcgatctctt tcaggagctc atcgaaactg aagtagagca
     5521 agcattcgat gccagcgtcc tggatctcac gagcggcatc ggggtcacgg agtaccgcca
     5581 ctctgggccg cggggtcatc gcgcgctcga ctgccgtgtc aatcgcatga tgcaggcgaa
     5641 cgagctctat gggctgtgga agaaagatat cggctccgag ctgagcgacc aacgcgcgag
     5701 tgtggggatc gtcatcgagc gagagcacga tcaccggcgt atcgctgcag ccgtgcaggc
     5761 tgcggagcat gggcagaaat cgggacagcg cgtcctgttc ctcgagcggc cagccaacca
     5821 gcactgcagt gggcgggttc cgcccagaga tcttgagtgc ctcctcgacg gtcgaaacgg
     5881 gcctcaagtg gacgagcacc tcatcgagcg cagatctgag gtaggcgatc atggccggat
     5941 cgtcctcgag cacgacgacg gtaggcacga aatcctgggg aatcgggcgg tgcaagaaga
     6001 agcttgcggc tggagtcacc gaaggtggtg ccgccgccgt caatcgaatg tcgaccgggc
     6061 gccccacggc cgtggccgcg tgagcccgta cggcgtcgat cgactcgaac atcgacgccc
     6121 aatcgaggcg acccgactcc tggagctgtc gaagctcggc ttcgattcgg cccaccgcat
     6181 cggaaatctc gccaaacccg tgggtctgcg ccgcgccgtg aaggtcgtgc gcttgccgaa
     6241 gcgcttcggc caggacgctc gttcggtctc cttcggaatg cacgcgacgg atcgcgccgg
     6301 tgaggttgtc cgccgcgatg ggaagaaggc tggtgtacgc ctgcttggct ttctcggcat
     6361 cgacaccagg ctcggaagcc tcgtcgggaa agacgatcgg atcctctggt tcgtccagca
     6421 gacgcgttac cacgggcgca aggctgtggg gggccgagat cgcgctagac acttcacgcg
     6481 cgaatcgatc gaccgtgacc ggcttgtgga agaccttgag aacgccgaga tcgcccgtga
     6541 gatgcttgaa cgtggtgagg tcgcgataga aggccgaaac gaagatgatc ggggtctcga
     6601 agccgtcgcg tcgaagctcc tcgatccacg tgatcccatt ggtgtcgggg agcaagccgt
     6661 ccacgacgag caaggagggt tcctcttcct cgagccaggc gcgcgcgatg cttcccttcg
     6721 cggcattcag tacgcgaaga ccaagagcct cgagcgcagg agtgacactc gagcggaact
     6781 gagtgtcgtc gtcaagcagg agaacggtcg ggggcacgtg agcgctagtg tggcaccgtt
     6841 tggggcgccc acaaaacgat tcgaccgaga ggctcaggac gcgcggcgga agtcggaatt
     6901 ggcttgcttg aggtatgccc gagcgaccgg atgttcagcg cactgctcga ccacggcgcg
     6961 aaggtgctga agcccctcct tccggtcgcc ggtctcaatc tcgatgacgc ccaggagcgc
     7021 gcgcgcatcg tggtgcgcag gttccagttg aagaatcagg gtcagttctt ctcgagcccc
     7081 ctgcgcgttg cccctcagtc gcagcagctt cgcgagctcg aggcggatgt cgtggaactt
     7141 ggggcaaagc cggagcgcgc gccggaactc gtgcatggcg tcttcggggc gatcgatctc
     7201 tagatatgcc cgcgcaagag agcgatgtag attcgcgacc ttcccccgac ggtatgcgtc
     7261 attggaagaa tccgaatgtc gctgggcccg ctcgagcgtg cgaacgccgg aaccatactc
     7321 gccgagctca ttgcaggtca cggtgaggtt gagcgcagcc tccatgtacc cgccgttgag
     7381 ctccagcgct tgctggaagc accagcgtgc ttcgggaagc cggcccccga ggtggtagat
     7441 caccccaagt cgattgaata cgtcgggata tcggagcccc tgctgtacga gcttctcata
     7501 gtagggttga gcgagcgggt aatcagcgcg ctcgtagtgc tcgttcgctg cgtcgagaat
     7561 ctgttgagcc cagggagcca tggacccgaa ggtagcgatg ggccggatcg ctggccagaa
     7621 atcggcgccc cgcgcgtcag ccgagcttac cgagcatcga tcgggccttc ttcgcctcgg
     7681 cgctgtcggg aaagcgctgg atgatctgat tgaaggtctc cttcgcggct tcgacctcct
     7741 tcgcctcgag gtagacctca ccgagcaaga gcaaagcctc ccgctcgacg ccgctgccgg
     7801 ggtaggcggt cagcaaacca ttgagtcgcg agatcactcc acgataggca tctcgcttga
     7861 gatagaaacg cgcgacgtag agctcgtggg cagccagcaa gctcatgcat ttttccatga
     7921 gctcgttggc tttcggaact cgctggtcgt ctgggtagtc gacgacgaac cggcgcaatt
     7981 ggatcagcgc gtcgcgtgct gcgctctgat cgcgttctgc cgcgggaggg gtcatgaacc
     8041 agccgccagg aatctgattg taataagcct cggcgatgcg aaatcgagcg taagggatct
     8101 ccttgtggga cggtcgaatc cgcacgaact gcctgtaggt ctggatggcc tcggggtacg
     8161 cctcattctt gaactggcag tccccgatgc ggagctcggc gagcgcggcg aacctgctgt
     8221 aaggaaactc gcggcgaacc tcgcgaaaca tcggttcagc cgcgaggcaa tcaccccgtc
     8281 tgaagtttaa gagcgccttc tcatagccgg agcgcgcatc gtcgtccgga tcgagcaact
     8341 tcctcgtgga gctcttcttc gacatcgagc tgttgccgca cgccatcgac gaggcgagaa
     8401 gcatgcagaa gacgaaccgg tagaggcgca accccatcga ccgggaacta gcttctgagg
     8461 cacgcccgaa caagccgacg gacaggggcc gagctccgag gcgttcaggc gctggcgatt
     8521 tgattgtcga tcaatcgggc cggatcgatg ccgagagtgg cgagcgcggc ttcccagcgg
     8581 ttttcgatcg gagtgtcgaa caccagggca gagtcgaggt cgactggaat ccaggagcct
     8641 tgtttcattt cgctttcgag ctggccggcg ccccagcccg agtagcccag gatcatggcg
     8701 caggtctcgg ggccgtctcc ccgagccatc cgtgccagga gctcgatcga ggcgctgcaa
     8761 gcgattcgtg gaccgactgc aatcgcctgg ccggacgaag cctgcggcac tccttcaggg
     8821 tcgtagagga gccaacccga ctccggtgaa accggcccgc cgagcatgac aggcgcgcac
     8881 acactggccg cggccgggcc ggtcacgcca acttcattga agacgtcggc aatgccgagc
     8941 tcggtcattc gattgacgac aaatccgaaa gagccctcgt tcccgtgatc gatcaacagc
     9001 accagcgtat gattgaagaa tgggcactgc atggacggcg ccgcgacgag gaaacccggt
     9061 gcaagtgatg ccgacacgag cgaagactgc caccaaatct gaaagctcgc gagcccaaat
     9121 tcagggacat cggaaatgag caaggtacgc gcggtcttgc gcacaggagc attcgtcggg
     9181 ttctccgctg ggatccttgc agcatacgag gcgagccgag cgctgacccc ggcatcggag
     9241 caagagaacc tcgccgatcg ctatcgggag cgattgactc gggggctcct ccgcgtattc
     9301 ggcgcgaagc tcttgatcga gggacactac agccatcggg gcggagcact cgtcgtgtcc
     9361 aatcatcaga gcgcgctcga catcggtgtc atgctctctc tgtttcgtgg cgtgctcgtg
     9421 agccgtcacg acgtggcgga ctggccgctg ctcggtcgcc tggcgaagca cggcgatacg
     9481 atcttcgtcg accgcgacga ccggcgcagc ggcgctgccg cgcttcgtga gattcggcgc
     9541 cgtgtgaagg aaggacgcac cgtcgtcgcg tttccagaag gaggcacgtt cccgggcgac
     9601 tccgtgcaag agttccatcc cggtgcgttc gccgccgtcc gctctctcgg cgtcccgatc
     9661 attccggttg ggctcgcata cgcaccgtcg gtgccttact cgggagaatc tttcggaagg
     9721 cacatcgcaa gagtcgcagc gcgcccgatg acccgcatcg ccgtgcacct cggcgagcct
     9781 cttcccggag acctcgacgc gcgcgcaatg gcagaagccg cacgcagtcg tgtagagagc
     9841 ctggtatccg atgcgcggcg cgcgctggac ggcgttcccg cctgaaatca gggggccccg
     9901 cagagatcgg tgcgaccttt tggtcgagcg gcctgctctt cgctaggatc cgctagtccg
     9961 cgccccaggg gccaccacag acaagcgcat ggttcgtctc attcagcaat cgaaggaaac
    10021 ggcgcccgac cgcgataatc tcggctggga attgcttcct agcgcgcttt gggacctcga
    10081 ctttcaacga ccgctcgaat ggatcgaagc ccgaggctgt cgcgatgcaa atgagttcaa
    10141 ggatctggtt cggaacgatc ccaacgctct ggccgagtgt ctcgaccgga ttcgcattca
    10201 tcgcgtgagc gctggtgcgg tggccttggt cggcgccgca agtgaggagg agtgcataag
    10261 acgctccctc gagcttctca ccgcagagtc caaagcagac tttcttcgcg tgctgatcgc
    10321 ggcctttcgc aaagagcacg acgtccacat cgaaaccgca atcatgacct gctcgcgcga
    10381 gccgcgggac gtgcgcgtgc attggcggtt cgttggaggt gaagagggac ggtttcagcg
    10441 cgcgatagtg tcggtcgtcg acatgacggc ggagcgggac ctgcaacagc gcctcttact
    10501 ggcagagcgg atgtcggcaa tcggaacgct caccgcgagc atcattcacg agatcaagaa
    10561 tccactcacc ttcgtgtgga atcacctgcg ctcacttcga gactccggag agcggctttc
    10621 cccagaaggc cgcgagctgc tggaggaggc ctacgagggc accgagcgaa tccgattgat
    10681 ttccaatgaa atcacgtctt tgtcgcatgg cgcggacggc gtcgaagtcg aaacggtgaa
    10741 cctcaaggag gtgctggacg ccgcgctgcg gatcgccggt ccggagatcc agcaccgggc
    10801 aaccgtggtg cgcgagtacg aagaaggcag cctatatatt cggggctcac gaacgcgcgt
    10861 cggtcaggtg ttcttgaatc tcatcgtcaa cgcggcccag gcgatcgacc ccggtgacct
    10921 ggcacagaac gcgatcaccg tccgggcgcg aagcaccgag agggaccgcg tttgcatcga
    10981 catacaagac acggggcccg gtatctcgcc ccaactcctg cggcgaatct tcgacccctt
    11041 cgtgacgacc aagccggcgg gccgcggaac cgggctcggg ctttcgatca cgcgccggat
    11101 cgtgcactcg ctcgaaggca caattgaaat tcaaagccac cctggacagg gcaccgtggc
    11161 ccgagtcgtc ctgccacgcg cgtcgcgtcc gcaacgtccg ctcacggtcc caccaccatc
    11221 catcagcgcg atccgacgtg ccgctcgggg caagttgtcg attctggtgg tggacgatga
    11281 accggtcatc gctcgactga ttcaaaaggc gctggtcgat cacgacgtca ccacagccca
    11341 cgatggccgc gaggcggtcg ccttgatggg agaacacgcc tacgatgtga ttctctgcga
    11401 tctgatcatg cccgaaatga ctggaatgga cgtgtaccgc gccgccctcc agcgcgcgaa
    11461 accgatgaac gaccgcatcg tgttcatgac cggaggcgcc ttcactcagc gggcgcgcga
    11521 cttcctcgaa agtgttccca acctccgaat tgagaagcct ttcgagctca cccacctcga
    11581 gcgtaccatt tacgaagcgg ccgacatcat cgatcctcac tagatggcga tgagcaggcc
    11641 gaagatgatc gtgatcgggc tcgactgtgc ggcgcccagt ttggtgttcg atcgctacca
    11701 agacgcgatg cctaacctcg gcgcattgat ggaacgagga acctggggac cgcttcgaag
    11761 ctcggagcct ccgatcaccg tgccggcctg gacctgcatg ctgtccgggc gcgatccagg
    11821 cgagctcggt ctctacgggt ttcgaaaccg tgtcgaagga gagactcggc tttgcctagc
    11881 ggacggtaag gacgttcgcg tgaagcgggt ctgggactgg cttggggagc acggataccg
    11941 ggtcgctccc ttgttcgttc cgctaaccta ccctccggct cccgttcgcg gtcgaatggt
    12001 ctcgggtttc atgtggccgg gcggagaggc accgtggtgc tttccacgcg gactcgaagc
    12061 cgagctcgtc gaacgctttg gtccgtacca ggcagacgtc gaagggtttc gggcaagcga
    12121 tctcgatcgc atctatcgcg acatcgtcgc aatgaccgag cagcacctct cgatcgccga
    12181 gcatatctgg gacgaggaga agccggactt catgatgatg gtagagatcg gtctagaccg
    12241 ctttcaccat gctttctggc gccatatcga cccggcgcat cctgggtacg acccggcgag
    12301 cccgtggaaa gacctgggcc tcgagtacta cggacggctc gatcagaaca tcggacgact
    12361 cctcgcacgg accgacgaag gcacgagcgt gctcgtcgta agcgaccacg gcgcacgcgc
    12421 catgcacggc ggcttctgca tcaacgaatg gttgagacgc aatggctacc tatcgctcat
    12481 ggacgtgccc aacgagccct ccgcactcga tcatgcccgc gtcgattggt cacgcacccg
    12541 cgcatgggca gaaggcggct actacgcacg agtatttctg aacgtcgagg gccgggagcc
    12601 aaaaggaatc gttgcccctg aacgaatacg atcgcttcgc acgcaattgc gcgaagagct
    12661 cgagaacgtg accgacgatg aagggaacgc gatcgcggtc tcggttcggg ttccggagga
    12721 agactaccgc gaggcgcgcg gtttcccacc cgacctgatg gtctacttcg atgatttgag
    12781 gctccgcgcg attggctcgg tcgggacggg ccgcatcagg gtcgcggaaa acgacacggg
    12841 acccgatggc tgcaatcacg actggagcgg catcttcgtc atgagcggcg gcggcacgct
    12901 cgcgcgcggc tacgtggacg gtgcggaaat ctacgacatc gcgcctacta tcttgggcgc
    12961 gtttggcatt ccccggccac cggagctttt cggcagagac tggacgaagt gaccatgact
    13021 ctagaagcct cgtcgagtcg cgcccgaagt gcaggctgct ccacgatgct ttcgcgctag
    13081 gcttcgccga tgggtgcgcg cgcgctagtg atcggcttgg acggcttcga cctggggctc
    13141 gtcgagcagt tcggtccgga gcgcctccca cacatccaca gtgtgatggc gagaggcgcg
    13201 ttcg
//
LOCUS       DASRZF010000145        10259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_443079, whole genome shotgun sequence.
ACCESSION   DASRZF010000145 DASRZF010000000
VERSION     DASRZF010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 10259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10259
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443079"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="VFG22_06615"
     CDS             <1..795
                     /locus_tag="VFG22_06615"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6415950.1"
                     /translation="EEKAELRRALDLDRPVGAQYLSFLGDMADGSMGTSFRQPDRSVW
                     SMMRDVLPHTIALAIAGLLVAWCVAIPLGTIAAARAGRAWDKVASTIAVAGLAIPNIW
                     LGPLLILAFGVKLRWLPLPGDDLAGPEALILPALTIGTALAAVLTRQTRAAMIEVLSQ
                     QYITAARARGVSSVALLLRHALRNALLPVITIGAAQLGALLSGTVVTEKIFERQGLGT
                     LFLDAFFARDIPVVQGCVLVIAVIYVGVNLAVDLTYGVIDPRVRLS"
     gene            792..1610
                     /locus_tag="VFG22_06620"
     CDS             792..1610
                     /locus_tag="VFG22_06620"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6415951.1"
                     /translation="MRTLRSRLRRTPIGLLIVLVFFFLGFIGPWIAPYDPLAIDLNHE
                     YATPSWTHWLGTGDNGIDILSALLHGARLAAIVGLVVVGVSVVIGTLLGTWAGYAGRM
                     TDHAVTGVADLVQAFPAIVLNIAVLALVARPGLIHLIVALSVNGWVLYARLARAETLS
                     LREREFVEAARALGVPTHAVLRRHVIPNLLGPLVIQATTGLGVVILAESTLSFLGLGP
                     GKAVSWGALLDQGTSVLLRFPHVALFSGGAIALTVLGFNLMGDWLRDRLDPRLR"
     gene            1809..3614
                     /locus_tag="VFG22_06625"
     CDS             1809..3614
                     /locus_tag="VFG22_06625"
                     /inference="COORDINATES: protein motif:HMM:NF015194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005471 - ATP:ADP antiporter activity
                     [Evidence IEA];
                     GO_process: GO:0006862 - nucleotide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Npt1/Npt2 family nucleotide transporter"
                     /protein_id="HET6415952.1"
                     /translation="MTAHSVMETARDTLFLTSLPATHLPRAYLAIAMLAIVELKVHER
                     VLRHVSDRRMLLSASLLFGAFVTLGFWALLDELGAWALFGFYVWTGLLITVVLIEFWL
                     LLDDAVTVTQAKRIFPAIAAGGVTGAMLGSLIADGLLRIAPPMSLVFAAALILALASA
                     APFLWKIPSESWAGRRGSEQGASLTWLLRDSYLSRVLMLVLMGTIALTVVDFVFKSAV
                     AENISAERLGHFFARFYFGLNSVALLVQLVGAGWLLRAFGVHRSSALLPVLIFGGAFG
                     LMLGPVLVFAVALKAVDGSLRHTLYRSSIEVLYLTLDSRQRERAKGIIEVFGHRVGQA
                     LASLLIIAAVGVGLSTPQFAAVLLFLVAAWIAAIMSTRHQYVNEFRARLRHGAVDLDI
                     EELIASDEDLVRSKILRALGRSQAHDRSVVFDDVLLQEQLRESLLRISQLLQWRAAIE
                     VEGMAETPDAELLRVALREKERAALERTFWIMDLWHAEENFNLVWRGLTSDNARLQAA
                     SREVLEAALSGSFREAVLAIIDDGESPGWRARAAATALGETVRPISRQEAMVQMVQDQ
                     SEVLRSMAISLLEARQPASPISPARGPRCHSKTLK"
     gene            3694..4326
                     /locus_tag="VFG22_06630"
     CDS             3694..4326
                     /locus_tag="VFG22_06630"
                     /inference="COORDINATES: protein motif:HMM:NF012381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415953.1"
                     /translation="MSSGTLIDFDPQEARAKALPLSELLPRARLVEVSGRHASARTTT
                     AVSCVIQAQAHAELVAWVQPKDGALFPPDLDAAGVDLDSFIVVHVPRHAGPFGLVRAA
                     EWLARSGAFGLTVIDLSDALPPGSSLNWQGRLQGLLRQCDGRILLLTSSAYEEPSSGP
                     LVGLRVEPHRSPLEADHFEVQMRVLKDKLGVASELSSTKPLAPSGLRGAS"
     gene            4323..5804
                     /locus_tag="VFG22_06635"
     CDS             4323..5804
                     /locus_tag="VFG22_06635"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415954.1"
                     /translation="MKRVSCVLLPMLPLQILLRQKPHWSGAPIAVVDDDGPNGRITHL
                     NALARKSRLQIGMRQTVARDLLPNLHTAVVSPERASKVSQELISALQTFSPRVEPLDR
                     AGAFYIDPEGLRRLYGSYHNWATSIHRYLRARHWRNAVTVGFHRHRSLAVVMMQNGVT
                     ILESAEEELAKSNQTPLREFDLSGELCENLAALGIDTLGDFLTLPAGELSSRFGSEAR
                     ALHDSFSDDMQLPIQPHAFDEPARISFQVDPPDEDQNRLLFAIKGALHSLLHQVRARG
                     EAIQSLEISLHLERAPLHREQLEPAAPTLDLMLLLELIRLRLGELDLRGAVEEVELLA
                     NTVRAEAEQVVLPGHRAHRDMGAAHRALARIRAAYGEQSVTKARMREAHLPEASFRWE
                     PVQHARLSERAPAQHASSMIRRVYARPKPLASRAPEQPEAGPALARDHAIEHMYGPYR
                     VSGGWWKRLVERDYYYAQTDHGDLLWLFYDRPRKRWFLHGVLD"
     gene            5805..8867
                     /locus_tag="VFG22_06640"
     CDS             5805..8867
                     /locus_tag="VFG22_06640"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HET6415955.1"
                     /translation="MSAYVPLWVKSNHSFLEGASFPEELVERAHALGLPAIAITDRDG
                     VYGLVRAHVRAKELGVRLIHGAQLSVEVDGHAHRIIAIAKTRRGYAGLCRALSLGHAR
                     CEKGMSLIESNDLAEVSEVFFLCGTPGALRLLHESQGPSSLFALCARHLSDHERPLEK
                     TLRSVAAELAVRTLGANEVLYHDKDRRPLQDVLSCIRNGNVLSEAGHRICANAEHDLK
                     ANDEFARLFADDPECLRRTIEVAEQCTFNLDDLRYRYPEESLPLGRTEQSWLSELTFR
                     GARARYDGKVPSDVRAQIERELHIIRKLDYGGYFLTMYEIVQYCREQGILCQGRGSAA
                     NSVVCFCLGITAIDPVRMDLLFERFLSVERAEPPDIDLDIEHDRREQVIQHVYEKYGR
                     RHAAMVANIIRYRIRSAVRDVGKVLGLSRTDLETATRLLRHRDDVLNPELLREAGLEE
                     DAPATRQLWSLASQILDFPRHLSIHPGGFLLGQRPVDEIVPIEPATMEARTVIQWDKY
                     DVDNLALFKVDLLGLGALSCIHHAFDLLREHKGQTLEIATVPPEDEATYDMVSRGETV
                     GVFQIESRAQMAMLPRLRPRTFYDLVIEVAIVRPGPIQGDMVHPYLRRRKGEEEIVYP
                     HPKLERILNKTLGVPIFQEQVMKLAVEAAGYTPGEADQLRRDMAAWRKSGRIEKHRTR
                     MIPRMLEEGIPEEFAERVFSQIRGFGEYGFPESHAASFALLAYVTAWLRCHHHDAFTC
                     AILNAQPMGFYTPATLIEDAKRHGVEVRPIDVNKSHWDCTLEESGVSAFSVRMGLRYV
                     KGLGVLERERLESGVLPYPNLATFVRQTRLNKKALHALAEAGAFECFGLNRRDAIWQV
                     RSLSRFARDSLPLEPASSQISFASLASDDEVIWDYRSSYHSTRGHPMRGLRHALHSLG
                     IPTATQIRARAHGEELQYVGMVICRQRPGTASGVTFFTLEDETGFVNVVLWKQVFDRH
                     AALAKTAALLGVTGEIQKADGVIHLIAKSLWAPDVSLAKQTHRSRDFH"
     gene            complement(8877..9293)
                     /locus_tag="VFG22_06645"
     CDS             complement(8877..9293)
                     /locus_tag="VFG22_06645"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HET6415956.1"
                     /translation="MSASALSLVGYAAWTLLLGVSIVFFRTGIVLAKKRAANDFLPSG
                     EDVSPFSNRLCRAHANCYENLPVFAALILLALVTGNEAITDPLARWVLVARVAQSLVH
                     LISTSEVAVSLRATLLLVQLGIEAYWIVQLGLLGFG"
     gene            9378..>10259
                     /locus_tag="VFG22_06650"
     CDS             9378..>10259
                     /locus_tag="VFG22_06650"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="catalyzes the interconversion of alanine and
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HET6415957.1"
                     /translation="MKTLLLTRSDVEAIATMELAVTAVETAFAAFGRGEATMPPKVYL
                     PIADHDGDFRAMPARLGESAGIKWVNVHARNPERYGLPTVMAVYVLNDPANAFPLAIM
                     DGTLLTALRTGAAGAVASKYLAQSSPKTIGFIGCGAQAYTLHGTHRAVFEQFETLAFD
                     RDAANGQRFAEAVDGRVVPLSEAANADIVCTATPSRAPLLEARWVRPGAHINAMGADA
                     PGKRELSLEILKSAAVYIDDIHQATSSGEINVPLANGDFTIDEIAGTLGEVVAGILPK
                     PELSTSTIFDSTGLAIQD"
BASE COUNT         1904 a         3151 c         3061 g         2143 t
ORIGIN      
        1 gaagaaaagg ctgagcttcg ccgagcgctc gatctcgacc gccctgtcgg agcgcagtac
       61 ctcagctttc tcggtgacat ggcggacgga agcatgggta cctcgtttcg gcagcccgat
      121 cgttcggttt ggtcgatgat gcgagacgtc ttgccccaca cgatcgcgct tgcgatcgcg
      181 ggcttgttgg tcgcctggtg cgtggcaatt ccgcttggca ccatcgccgc agcccgggcg
      241 ggaagagctt gggacaaggt cgcgtcgacg atagcggtcg cgggtctcgc cattccgaac
      301 atctggctgg gaccgctgct aatcctggcg ttcggagtga agcttcgttg gctgcccttg
      361 ccgggcgacg accttgctgg ccccgaggcg ctgatccttc cggcgctcac gatcggaacg
      421 gcgctggccg cggtgctcac tcgccaaacg cgagccgcga tgatcgaggt tctatcccag
      481 cagtacatca cggccgcccg cgcccgtggg gtttcctcgg tcgcgcttct gctgcggcat
      541 gcgcttcgaa atgccctgtt gccggtcatc acgatcggtg cggctcagct cggggcgctc
      601 ttgtccggaa ccgtcgtcac agagaagatc ttcgagcgac aggggcttgg aaccctcttt
      661 ctcgatgcgt ttttcgcgcg tgacattccc gtcgttcagg gctgtgtgct cgtcatcgcg
      721 gtgatctacg tgggtgtcaa tctggccgtc gacctgacgt acggcgtgat cgacccgcgg
      781 gtgaggctct cgtgagaacg ctgcggagtc ggcttcggag gaccccgatc ggactcttga
      841 tcgtgctcgt attcttcttt ctaggcttca ttggaccgtg gatcgcgccc tacgatccac
      901 tcgccatcga cctgaaccac gaatacgcca ctccttcctg gacacactgg ctcggcacgg
      961 gtgacaacgg catcgacatc ttgagtgcct tgctgcacgg ggcgagactt gcggcgatag
     1021 tcggtctcgt cgtcgtggga gtctcggtcg tcatcggcac cttgctcggt acctgggccg
     1081 gatacgccgg acgcatgaca gatcatgccg tgaccggcgt cgcagatcta gttcaggcgt
     1141 ttcccgcaat cgtcctgaac atagcggttc tcgctttggt tgctcgtccc ggcctgatcc
     1201 atttgatcgt cgcgctctcg gtcaacggat gggtgctcta tgcgcgattg gcccgagccg
     1261 aaacccttag cctccgcgag cgtgagttcg tcgaagccgc gcgtgcgctc ggcgttccta
     1321 cccatgcagt gctcaggcgc cacgtcattc cgaatctgct cgggccgctg gtcattcagg
     1381 ccacgaccgg actgggggtc gtgatcctag cggagtcgac tctctcgttc ctcggactcg
     1441 gacctgggaa agccgtatcc tggggcgcgc ttctcgatca aggcaccagc gtgctcttgc
     1501 gattcccgca cgtggcgctt ttctccggcg gcgcgattgc gctgaccgtg ctgggcttca
     1561 acctgatggg cgactggttg cgcgaccggc tcgacccacg cctcagatag ccatgccgga
     1621 gcttccagat gtggagctct accttcattc gctccgctga cggttgacag gacgagcact
     1681 cgacccgcag cgcacggaaa tgaccggagc gcaattagac gttaggttgc ggcggacaca
     1741 tgcgcttttc cgttcgccgc gaggaccgaa ggaatctcgt cgtaggcttt atcggcttgc
     1801 tttcggtgat gaccgcgcat tccgtcatgg agacggcgcg cgatacgctg tttctgacga
     1861 gccttcccgc aacgcatttg cctcgggcgt atctggcgat cgcgatgctg gccattgtcg
     1921 agctcaaggt tcacgaacgg gttcttcggc acgtgagcga ccgaaggatg ctcttgtcgg
     1981 cgtctctcct gtttggggcc ttcgtgacac tcggtttctg ggcgctgctc gatgagctgg
     2041 gcgcatgggc ccttttcggc ttctacgtct ggacggggct gctgatcacc gtggtcctaa
     2101 tcgagttctg gctcttgttg gacgacgcgg tcaccgtgac ccaggcgaag cggatcttcc
     2161 ccgcaattgc cgcgggaggc gtcacgggtg ccatgttggg ttcgctgatc gccgacggtt
     2221 tgctgcgcat cgctccgccg atgtcgctcg tgttcgcggc ggcgctgatc ttggcgctcg
     2281 cttcagcggc gccgttcctc tggaagatac cgagcgagtc ctgggcgggg cgtcgcgggt
     2341 cggagcaggg cgcatcgctt acttggctgc tcagggatag ctatttgtcg cgcgtcctca
     2401 tgctcgtctt gatgggcacc atcgcgctca ctgtcgtcga tttcgtcttc aagagcgcgg
     2461 tcgccgagaa catttctgct gaacggctcg ggcatttctt cgcgcgcttc tatttcggac
     2521 tgaactccgt ggcgcttctc gtgcaactgg tcggggccgg ctggcttctt cgcgcgttcg
     2581 gcgtccaccg cagctcggcc ctcttgcccg ttctgatctt cggcggtgct ttcggtctaa
     2641 tgctcggacc ggttctggtg tttgcagtcg cgctcaaggc cgtcgacggg agccttcggc
     2701 atacgctcta tcgaagctcc atcgaggtgc tctacttgac cctcgacagt cgccagcgcg
     2761 agcgggctaa aggcatcatc gaggtgttcg ggcatcgtgt ggggcaagcg ctcgcatcct
     2821 tactgatcat cgccgccgtc ggagtcggcc tttcgacccc gcaattcgct gccgtattgc
     2881 tattcctggt ggctgcctgg atcgctgcga tcatgtcgac gcgtcaccag tacgtgaacg
     2941 agtttcgcgc acggctccgc catggagccg tcgacctcga tatcgaggag ctcatcgcga
     3001 gcgatgaaga cttggtgcgc tcgaagatct tgcgcgcact cggccgctcg caggcgcacg
     3061 atcgatcagt cgtgttcgac gacgttctct tacaagagca gcttcgcgaa agcttgctgc
     3121 gtatcagtca gctccttcag tggcgcgctg caatcgaggt cgaaggcatg gcggagacgc
     3181 cagatgccga gcttctgagg gtcgcgcttc gcgagaagga acgggcggcg ctcgaacgca
     3241 ccttctggat catggacctc tggcatgcag aggagaattt caatctggtt tggaggggtc
     3301 tgacgagcga caacgcgcga ctgcaggccg caagccgtga ggtgctggaa gcagccttga
     3361 gcggctcgtt ccgagaagcc gtgctcgcga tcatcgacga tggggagtcg cctggatggc
     3421 gggcgcgtgc tgcggcgacc gcgctcggag agacggttcg cccgatctcc cgccaggagg
     3481 cgatggtgca gatggtgcaa gaccagagcg aagtcttgcg cagcatggcc atatccctcc
     3541 tcgaagcacg gcagccggca tccccaatct ctccggccag gggccctcgt tgccattcta
     3601 agacactgaa ataaaaggcc tttcaatcaa aagttctctg ctgggcatct ctctcttgta
     3661 actgttaata cgttcagtat attgggtggt cccatgtctt ctgggactct catcgatttc
     3721 gatccgcagg aagcccgcgc gaaggctttg cccctttcgg agctcttgcc acgggcccgg
     3781 ctggtcgagg tctcagggcg gcatgcttcg gcccgaacga cgacagccgt gtcgtgcgtg
     3841 atccaggccc aagcgcacgc tgagctcgtg gcctgggtgc aacccaagga cggcgccttg
     3901 tttcctccgg atctggacgc ggcaggagtc gacctcgact cgttcatcgt agttcacgtt
     3961 ccgcgtcatg caggcccgtt cggcttggtt cgtgctgccg aatggctggc tcgttccggt
     4021 gcgtttggac tgacagtcat cgatctcagt gatgcactgc cacctggctc ttcgctcaac
     4081 tggcaaggcc gccttcaagg tctcttgcgt caatgcgacg gacgcattct cttgctgacc
     4141 tcgagcgcat acgaagaacc ttcttccggt ccattggttg gcttgcgtgt cgagccccat
     4201 cgcagccctc tcgaggcgga tcatttcgaa gtacagatgc gcgttctcaa agataagctc
     4261 ggggtcgctt cagagctttc atcgacgaag cccctcgcgc cgtctggttt acgaggcgct
     4321 tcgtgaagcg ggtgagctgc gtccttttgc cgatgcttcc cctacagatc ctgttgcggc
     4381 aaaaaccgca ctggagcggc gcgcccattg ccgtggtcga tgacgacggc cccaacggcc
     4441 gcattacgca tctcaacgcg ctcgcaagaa agagccgcct ccagatcggc atgcgccaaa
     4501 ccgtcgctcg tgatctcctg cctaaccttc acacggcagt cgtctccccc gaacgtgcat
     4561 cgaaagtcag ccaagagctt atcagcgcgc ttcagacctt ctcaccacgg gtcgagccgc
     4621 tcgatcgtgc cggcgcattt tacatcgatc cggaaggtct ccggcggctc tatggcagct
     4681 atcacaactg ggcgacatcc attcaccgat atttgcgcgc tcggcactgg cgcaatgcgg
     4741 tgaccgtcgg ctttcatcgc catcgctcgt tggcggtcgt gatgatgcag aatggagtca
     4801 ccatcctaga gagcgccgaa gaggagctag cgaagtcgaa tcagacgcct cttcgtgaat
     4861 tcgatctgtc gggtgagctt tgcgaaaatt tggcagcgct gggaatcgat acactgggcg
     4921 atttcctcac tctgcccgct ggtgagctct cgagccgttt cggttccgag gcacgcgcgc
     4981 ttcacgattc gttctctgat gacatgcagc ttcccattca accgcatgcc ttcgatgagc
     5041 cagctcgtat ctctttccaa gtcgatccac cggacgaaga tcagaaccgt ctactcttcg
     5101 caatcaaggg cgctctgcac tctttactgc atcaagttcg cgcccgtggt gaagccattc
     5161 aatctctcga gatttcgctt cacttggaac gggcgcctct tcatcgagag caattagaac
     5221 ctgctgcgcc aaccttggat ctgatgctgc tgctcgagct cattcgtctt cgcctcggcg
     5281 agctcgactt gagaggcgcc gtcgaagaag tggagcttct cgccaacacg gtgcgtgccg
     5341 aggcagagca ggtcgttctt cccggccatc gggctcaccg tgacatgggc gctgcacacc
     5401 gtgcacttgc tagaatccgt gctgcatacg gcgagcaatc cgtgacgaag gcccgcatgc
     5461 gcgaggcgca cttaccggaa gcctcctttc gatgggagcc cgtccaacac gcacggctca
     5521 gcgagcgcgc cccagctcag catgcttctt ccatgattcg gagggtttat gctcggccca
     5581 agccacttgc ctcacgcgca ccggagcagc ccgaggccgg tcccgcgctc gcgagggacc
     5641 atgcgatcga gcacatgtac gggccatacc gcgtgagcgg cggttggtgg aagcggctcg
     5701 tagagcgcga ctactactac gcccaaaccg atcacggcga cttgctctgg ctcttctacg
     5761 accgtccccg gaagcgttgg ttcttgcatg gagtactcga ttgaatgagc gcctatgttc
     5821 cgttgtgggt caagagcaac cacagtttct tggaaggcgc ttcctttcct gaagagttgg
     5881 tggaacgggc acatgcgctt ggtcttcctg ccatcgcaat tacggaccgt gacggagttt
     5941 acgggctagt acgcgcgcat gtgcgagcca aagagctcgg tgttcgcctc attcatggag
     6001 cgcagctcag cgtcgaagtc gacggccatg ctcatcgaat catcgcaatt gcaaagacac
     6061 gacgtggcta tgccggccta tgcagggcct tgtcgctcgg acatgcgcgc tgcgaaaaag
     6121 gcatgtctct catcgagtcc aatgacctcg ctgaggtgtc cgaagtgttt ttcctctgcg
     6181 ggacacccgg agcactgcgc ctcttacatg agagccaagg cccctcatcc ttgtttgctc
     6241 tctgcgcccg tcacctcagc gaccacgaga gaccactcga gaagaccttg cgctcggtcg
     6301 cggccgagct cgccgtgcgg acgttggggg ccaacgaagt cctctatcac gacaaagacc
     6361 gacgccctct tcaggacgtt ttgtcctgta ttcgcaatgg aaacgtgctt tccgaggccg
     6421 gtcacaggat ttgcgccaat gcagagcacg acctcaaagc caatgacgaa ttcgcgaggc
     6481 tctttgcgga tgacccggag tgccttcggc ggaccatcga ggtggcagag caatgcacgt
     6541 tcaacctcga cgatcttcgc tatcggtatc cagaggaatc gcttcctttg ggacgaaccg
     6601 aacaaagctg gttgagtgag ctcaccttcc gaggcgctcg cgctcgttac gatggcaagg
     6661 tcccttccga tgttcgagca caaatcgaac gcgagcttca catcatccga aagctcgatt
     6721 acggcggcta ctttctaacc atgtacgaga tcgttcaata ctgccgggag caaggtatct
     6781 tgtgccaagg ccgagggagc gcagccaaca gcgtcgtgtg tttctgtttg ggcatcacgg
     6841 caatcgaccc ggtgcgcatg gacttgttgt tcgaacgttt cttgagcgtc gagcgtgccg
     6901 agcctcctga catcgatctc gacatcgagc atgaccgacg tgaacaagtg attcagcacg
     6961 tctacgagaa gtacggacgg cgtcatgccg caatggtcgc gaacatcatc cgctatcgca
     7021 tccgatccgc agtgcgcgac gtaggcaagg tgctcggctt gtcgcgaacc gatctagaaa
     7081 ccgccacccg cttgctgagg catcgcgacg acgttctcaa ccccgagttg ctccgagaag
     7141 ccgggctcga agaagacgca cccgccacgc ggcaactctg gtctctagcc tcgcaaatac
     7201 tcgattttcc tcggcatttg tcgattcatc cgggcggttt cttgctgggc cagcgccccg
     7261 tcgacgaaat cgttcccatc gagccggcca ccatggaagc gcgcacggtc atccagtggg
     7321 acaagtacga cgtcgacaat ctcgctctgt tcaaggtcga cctcttgggc cttggagcgc
     7381 tctcttgcat tcatcatgcg ttcgatctgc tgcgcgagca caaagggcag accctcgaga
     7441 tcgcaaccgt ccctccggaa gacgaagcca cgtatgacat ggtgtccagg ggcgagactg
     7501 tgggcgtctt tcaaatcgaa agccgtgccc agatggccat gcttccgcga cttcgaccgc
     7561 gcacgtttta cgacctggtg atcgaagtag cgatcgtacg accgggtccg attcaaggcg
     7621 acatggtgca cccttactta cgaagacgaa agggtgagga agaaatcgtc tatccgcatc
     7681 ccaagctcga acgcatcttg aataaaacac tcggcgttcc gatctttcaa gagcaagtca
     7741 tgaagctcgc ggttgaagcg gcaggttata cgccgggcga agcggatcag ttgcgccgtg
     7801 acatggctgc atggcgaaag tccggacgga tcgaaaagca tcgcacgcgc atgattccgc
     7861 gtatgctcga agaaggtatt cccgaagagt ttgccgaacg tgtgttttca cagattcgtg
     7921 gattcgggga gtatggcttc ccagaaagtc acgcggcctc gtttgctttg ctggcgtacg
     7981 tcaccgcctg gttacggtgc caccaccacg acgcatttac ctgcgcgatc ctgaacgcac
     8041 agccgatggg gttctacaca ccagcgaccc tcatcgaaga tgccaagcgg cacggtgtcg
     8101 aggtccgacc gatcgacgtc aacaagagcc attgggattg cacattggaa gagtctggag
     8161 tttctgcgtt ctcggtcagg atgggccttc gatacgtaaa agggctcggc gtactcgagc
     8221 gagagcgcct cgagtcgggg gttctgccat atccgaacct ggcgaccttc gttcgccaaa
     8281 cgcggctcaa caagaaagcc ctccacgcct tggccgaggc cggcgcattc gaatgctttg
     8341 ggctcaaccg acgcgacgcg atctggcagg tccgcagtct ctcgcgtttc gcgcgggatt
     8401 cattgccact cgaaccagca tcgagtcaga tctcgttcgc atcgctcgca agcgatgacg
     8461 aggtgatctg ggactaccgt tcgagttacc acagcacgag gggtcacccc atgcgagggc
     8521 ttcgtcacgc gcttcattca ctcggcattc ccacagccac gcaaatccgt gctcgtgcgc
     8581 atggtgaaga gctccaatac gtcggcatgg tgatctgccg ccaacgcccg ggcacggcat
     8641 ccggcgtcac cttcttcacg ctcgaagacg aaacgggctt cgtcaacgtg gtgctttgga
     8701 agcaggtgtt cgatcgtcac gctgctcttg ccaagaccgc agccctcctc ggggtgacgg
     8761 gcgaaatcca aaaagccgac ggcgtcattc acctgatcgc caaatcatta tgggcgccgg
     8821 acgtttcgct ggccaaacag acccaccgct ccagggactt ccactgatgc ggtcgatcag
     8881 ccgaaaccaa gaagcccgag ctggacgatc cagtacgcct cgatacccag ttgcacgagc
     8941 agcagggtcg cgcgcagcga taccgccacc tcgctcgtgg agatcaagtg cacgagagat
     9001 tgcgcgaccc gagccaccag cacccatcgg gccaaggggt cggtgatcgc ctcgttaccc
     9061 gtgacgagcg cgagaagaat cagagccgcg aagaccggaa ggttctcgta gcagttcgca
     9121 tgcgcgcggc agagtctgtt cgaaaacgga gagacgtcct caccgctcgg aaggaagtcg
     9181 ttcgccgcgc gctttttcgc gagcacgatc ccggttcgaa agaatacgat cgaaacgccc
     9241 aataggagcg tccacgctgc atacccaacc aacgaaagcg ccgatgcgga catcatcatt
     9301 ctcctagtga cgtcgagacc ggcggactat acccaagccg ccaggcgctg gatagggtct
     9361 gaggggaggg tcgactcatg aaaacgctct tgctgacgag gtcggacgtc gaagccattg
     9421 cgacgatgga acttgccgtg acagccgtgg aaacggcttt cgccgcattc ggcaggggcg
     9481 aagcgacgat gccaccgaaa gtctacctgc cgatcgcgga ccacgacggg gactttcgcg
     9541 caatgccggc gcgcctcggt gaatccgcgg gaatcaagtg ggtgaacgtc catgctcgaa
     9601 atcccgagcg ctacgggctg ccgaccgtga tggcggtcta cgtgctcaac gacccggcca
     9661 acgcgttccc gctggcgatc atggatggca ccttgctgac cgcactgcgc accggagctg
     9721 ccggcgcagt tgcatcgaag tacctcgcgc aaagctcgcc gaagacgatc gggttcatcg
     9781 gctgcggagc gcaggcctac acactccacg gcacccatcg agccgtcttc gaacagttcg
     9841 agaccctcgc gttcgatcgt gacgctgcga acgggcaacg gttcgcagag gcagtggacg
     9901 gccgggtcgt accactgagc gaagccgcaa acgccgacat cgtctgcacg gctacaccgt
     9961 cgcgagcacc gttattggag gcgagatggg tgcggccagg agctcacatc aatgccatgg
    10021 gtgccgacgc cccgggcaag cgcgagctca gtctcgaaat cctcaagagt gcggccgtct
    10081 acatcgacga cattcaccag gcgaccagca gcggcgagat caacgttccc ctcgcgaatg
    10141 gggatttcac catcgacgag atcgcgggca ccttgggcga agtcgtagcg gggatcctgc
    10201 ctaagccgga actgagtacc agcacgattt tcgattcgac cggtctcgcg attcaagac
//
LOCUS       DASRZF010000146         9038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152 k141_98147,
            whole genome shotgun sequence.
ACCESSION   DASRZF010000146 DASRZF010000000
VERSION     DASRZF010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 9038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9038
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98147"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VFG22_06655"
     CDS             <1..253
                     /locus_tag="VFG22_06655"
                     /inference="COORDINATES: protein motif:HMM:NF014522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HET6415958.1"
                     /translation="ENLVKPENVATLRNILQYHVTTSALQLDWFKDGQTVSMANGKRT
                     TMNVKDGKVTVNGANILATIPAENGIVYVIDAVLLPPEK"
     gene            333..1211
                     /locus_tag="VFG22_06660"
     CDS             333..1211
                     /locus_tag="VFG22_06660"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6415959.1"
                     /translation="MSKLEGKRVLITGGAQGIGFEMAIKFAERGAAIVLADIDEEKLV
                     EAKAKLEERGITASGFRVDVTNPASIASLRAQINAEAGPIDVLVNNAGVVFGGPFTKT
                     SLDDHFKTYEVNVLGLIAMTHAFLDDLVARPEAHLVHIASASGFIGLPYGSTYASSKW
                     AVIGFGESIRAELNLQGHEHVHHTIVCPSYIGTGMFEGAEAPKATQILEPDYLAEKVV
                     QAVERNKLHVLEPFMVKLTPLIRNLLPTALYDRISHLFGADTSMAHWTGRDGDAVTTD
                     ESKPQSAGQDQAEAKR"
     gene            complement(1208..1987)
                     /locus_tag="VFG22_06665"
     CDS             complement(1208..1987)
                     /locus_tag="VFG22_06665"
                     /inference="COORDINATES: protein motif:HMM:NF044908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HET6415960.1"
                     /translation="MDGEAFFRQLDHGLYEATEWTRGPWNPKHQHAGPPSALVCGRLE
                     EIAGDALRVVRVAIEIPRPVPIARLRLETSVRRSGRSVTAIVGQLFDQEGKLVMNAEA
                     LALSRAEIGIPAPRPQMDELLPSESSPIEFPFHDSKPGYAAGMELRFARGTFGKGDVM
                     AWMRMRLPLLSDADLSPLERTLIAADSGNGVSQRVSPFEYTFLNADLTVTLHHPAEGE
                     WIGMAARTDFDIDGVGVADTRLYDARGPIGRGIQTLLIRKR"
     gene            2627..2702
                     /locus_tag="VFG22_06670"
     tRNA            2627..2702
                     /locus_tag="VFG22_06670"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2660..2662,aa:Asn,seq:gtt)
     gene            2832..3917
                     /locus_tag="VFG22_06675"
     CDS             2832..3917
                     /locus_tag="VFG22_06675"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HET6415961.1"
                     /translation="MEYLKPIIGLLRDWLPVLITVLVAIVVVWLAYWLLFKRRSPSSV
                     SRISQQLFIAALIGIFLVIVILELPIGDAPRGQLLSLLGLLVTAAIALSSTTLLGNAM
                     AGLMLRSVRNFKPGDFIIVDSHRGRVSELGLLRTEIQTDKRNLTTLPNLYLINNPVTV
                     VRPSGTFIAATVSLGYDVPRHSVEECLVKASENAGLTDPFVFVLELGDFSITYQTAGF
                     LEEVKYLISAESKLRECMLDALHEAGIEIVSPTFMNQRQLSEDRRFIPRNERHTVAKP
                     AAEEPRPEELMFDKAEVAESEAKATERLKAVVEEMEKIQKNEIKELSKEAAAARLEEL
                     QIARSKLELEIEARKEQKKEEPSDEGA"
     gene            complement(3914..5344)
                     /locus_tag="VFG22_06680"
     CDS             complement(3914..5344)
                     /locus_tag="VFG22_06680"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET6415962.1"
                     /translation="MTPRTRWIRILMGSVASVLLLNATRAQSQPLQFDEVLSSVTAHD
                     PRIRRAVEEVRRAKGVTTEARGAFDPRIDGDLSLTGGAYYDLRSANAELRQPTSLWGS
                     EIYVGYRVGVSIDQRWPTYRSDQTLSGGELRAGIELPIWRGGVIDEPRAERARAIHLE
                     EAAEEDLSSAALNLELAAAGAYWSWVSAGQKLEVTKALLALAEDRDTQLRRRLAAGSI
                     AEFDVTDNERILLERRAFLVSAQRSFEQAAFGLSLFLRDPAGRSVVPDPSRLPKEAVR
                     SAEPELAEDLVQSRVLACHPELGSARAKLDAAEIDVKLTRNQLAPELRGRFQYSRDLG
                     ELTGTDLDFTLPGNVFQAGLELSMPLLFRDERGRASAARAKVAASRAGIDLLEDQLVA
                     RMRDAASAVRAAHERVELTQQVVGTAEELAKGERRRFEFGSSNLIFVNLREQQAAMAR
                     VQFIDAVAVAEIERTRWETTTQVECR"
     gene            complement(5341..6687)
                     /locus_tag="VFG22_06685"
     CDS             complement(5341..6687)
                     /locus_tag="VFG22_06685"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HET6415963.1"
                     /translation="MTEVNRAIEETQHWTGFEQNEIPSGLEVPAPQRAASVAKLLVSA
                     FVLFTLAALLAPWTQNVRGRGRVVAYAPDKREQAIEASVSGVVAEWFVEEGSTVSKGD
                     PIVRLEDNDERILDRLGAQRAAIEVQRIAQGQRLETLTSRVESLRRSQRAEISGAESS
                     IAISQQDVAAAQQDLGAAQAELDTNELNLRRQRDLSRDGLVSQRELELAELAVRTSKA
                     QVASANADLSAARNRLSQSRAALRRVIASTEAEIENAEAGLRSAETEVASANGSLSRL
                     EVDIARQQDQVIRAPIEGTIVKVVGRLGGEQVSRGELLAVLVPTTEDRAVALYVDGND
                     AALITPGREVRLQFEGWPAVQFSGWPSVAVGTFGGRVAFVDATDDGRGDFRIVVVPDP
                     NDGPWPAASYLRQGVLAKGWILLDRVRLGFEVWRQFNGFPPTTAPPPMASQNEGAK"
     gene            complement(6684..8363)
                     /locus_tag="VFG22_06690"
     CDS             complement(6684..8363)
                     /locus_tag="VFG22_06690"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6415964.1"
                     /translation="MNQESLAAGRPSPLRRTRQLLELDRQDIVVIIIYGVALGLLSLA
                     VPIAVQSLVNTVAFGSLIQPLIVLTVLLAVALIGSAVLRALQVRIVEMLQRRLFVRIV
                     AEISGRLPRVSAKALEAANGPELLNRFFDVFTTQKSVASLLLGGIDALLIALVGLVVL
                     AFYHPALLAFDIVLIIGAVIVLIPLGRGGTRSSVEESKAKYAVAGWLEEIARHPLSFK
                     LAGGGEFAKERAQDLARSYLHKRERHFRVVFRQLIGALTIEVVASVALLAVGGLLVIE
                     RQLSIGQLVAAELIVTAVVASIAKLGGKVEIFYDLVAAVDKLGDIFDLPLERGNGESP
                     PPNQGSQGRLVLTQVEIETEDGSAPGPVSLEIRPGEWIGLSGASERVTTSLVDAVFGL
                     NAPKSGTIELDGHDVRDLALTDVRSRVTLVRGTEILPGCIADNLHAANPELAADQAWN
                     ALRRVGLDEWVKDSALGLDTKILPHGAPLTRSQGLALTIARALAGKPSILILDRTLDR
                     LGPSERIRVLDALLVDRDVSVLLVSDEPQVLARTDRLIDLSEMIKPQGEAA"
     gene            8444..8740
                     /locus_tag="VFG22_06695"
     CDS             8444..8740
                     /locus_tag="VFG22_06695"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET6415965.1"
                     /translation="MSIDPSTPPWTFLSNHGHILLSIARDPNIRVREIARSVGITERA
                     VQRILGELEEAGVISRLRQGRRTHYEIDRSQPLRHPVEAAHSVGELLRLARSRR"
     gene            complement(8746..>9038)
                     /locus_tag="VFG22_06700"
     CDS             complement(8746..>9038)
                     /locus_tag="VFG22_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415966.1"
                     /translation="NPQNDFFIPLFTYADFDITGDIDLTLSWFTNLVVTTIGNTNHTG
                     KADLAIELNSVIDLDIAFLFFRTEDPAPSSDPDVTVYKNDYQLVIGVSLELG"
BASE COUNT         1757 a         2814 c         2852 g         1615 t
ORIGIN      
        1 cgagaacctg gtcaagccag agaacgtggc cacgctgagg aacatcctgc aataccacgt
       61 caccacgtcg gcacttcagc tcgattggtt caaggacggc cagaccgtct cgatggccaa
      121 cggcaagcgg acgacgatga acgtgaagga tggcaaggtg acggtgaacg gcgccaacat
      181 cctcgccacg atccccgccg aaaacgggat cgtgtatgtc atcgacgccg tgctccttcc
      241 gccggagaaa taggccgcgc cgacgttaaa accgccggga tccagatccg gatcgcccca
      301 ggcaactaga gtcctgctac catccgctca ccatgagcaa attggaaggc aagcgggtgc
      361 tgatcacagg cggcgcccag ggcatcggct tcgagatggc gatcaaattc gccgagcgcg
      421 gagcagcgat cgtccttgcg gacatcgacg aagaaaagct cgtcgaggcc aaggccaagc
      481 tcgaggagcg cggcatcacg gcctcgggct tccgcgttga cgtcaccaac ccggcgagca
      541 tcgcgtcgct gagagctcag atcaacgccg aggccggacc gatcgacgta ctggtcaaca
      601 atgccggagt cgtcttcgga ggtccgttca ccaagacctc gctcgacgat catttcaaga
      661 cctacgaagt caacgtcctg ggcctgatcg ccatgacgca tgcgtttctc gacgatctgg
      721 tcgcacgccc cgaagcccac ctcgtacaca tcgcgagcgc atcgggattc atcggcctcc
      781 cgtacggatc gacgtatgcg tcgagcaaat gggccgtcat tggtttcggc gagtcgatcc
      841 gagccgagct caacctgcag ggacacgagc acgttcatca caccatcgtg tgtccgagct
      901 atatcggcac gggcatgttc gaaggtgccg aggcgccgaa ggcgacgcag atcctcgagc
      961 cggactacct ggccgagaag gtcgtccagg ccgtcgagcg aaacaagctc cacgtcttgg
     1021 agccgttcat ggtgaagctc acgcccttga tccggaacct cttgccgaca gcgctctatg
     1081 atcggatctc acacctcttc ggcgcggaca cctcgatggc gcattggacg gggcgcgacg
     1141 gcgacgccgt gacgacggac gaatccaagc cgcaatccgc cggtcaggat caggcggaag
     1201 ccaaacgcta gcgcttgcgg atcagtaggg tctggatgcc gcggccgatc gggccacgcg
     1261 catcgtagag acgggtgtcc gcgacgccaa cgccatcgat gtcgaaatca gttctggcgg
     1321 ccatcccgat ccattcgccc tcggccggat gatggagtgt caccgtgagg tcggcgttga
     1381 ggaatgtgta ctcgaaaggg ctcacccgct ggctcacgcc attgccgctg tccgcggcga
     1441 tgagggtgcg ttcgagtggc gagagatccg catcgctgag tagcggtagc cgcatgcgca
     1501 tccaggccat cacgtcgccc ttcccgaacg tgccccgcgc aaagcgaagc tccatccccg
     1561 ccgcgtagcc gggcttcgag tcatggaagg ggaattcgat cggcgaagat tcgctgggaa
     1621 gaagctcatc catctgagga cggggcgcgg ggattccaat ctcggcgcgg gagagcgcga
     1681 gcgcctccgc gttcatgacg agcttaccct cctggtcgaa gagttgaccg acgatcgcgg
     1741 tcacggaacg cccggaccga cgaaccgagg tctcgagtcg aagcctcgca atcggcacgg
     1801 gtcgcggaat ctcgatcgcc acgcgcacga cccgcaaggc atcaccagcg atctcttcca
     1861 agcgcccaca aacgagggcc gatggcggcc ccgcatgctg atgcttcggg ttccagggtc
     1921 cgcgcgtcca ctccgtcgcc tcgtagagcc catgatcgag ctgtcgaaag aacgcttcgc
     1981 cgtccatgaa cgagaatctg gcacgcagac gcgtggacga aacccgaaag gcacgcagga
     2041 gtgagctgca gcagcgcatg tggagggcgc tgacactggg agagtgatgc tttcagctgt
     2101 ttggatcgga gagcggctcg cgacgcacgt gctcgcgatt gggacccgct cggccggctc
     2161 tccaagattc cctacgtctc gcctcccgcc caatcgctgt gccgcgcatc ccgagccgcc
     2221 ctcctattta agcaccgaag catgaaccct ggcactggct tacgtagggg tggggcggaa
     2281 cgcgatgcgg ggttccggcg acgtcgcgaa tgacaatcgg tcagggagca accagccccg
     2341 attggcatga gcgccgaggc gaggcttgcg tggcccccgg ccccaagtcc caccggtcac
     2401 aattgcaagc cgagccgttc gacggaactc cgtgtcgtgt tccgccccgc cccgatcgcc
     2461 caatcgcgag cacgcccgtc gcgagccgtc ctccgcttgc tcggtggcgc aagtcagttg
     2521 ctggcgctgg cggatctacc gcggaagatg ggaatctgtg gggcagaatt cagaacgaat
     2581 tcccttgctt gacaggcgcg caaggcacgc tatcaatcgc agccattcca gcgtagctca
     2641 gttggtagag cgggcgactg ttaatcgctt ggtcataggt tcgagtccta tcgctggagc
     2701 catctaccgg gccgtctcaa gagcgtgagg acctttttcc tttcgcatac cggggcgtgg
     2761 caagtctcga accaacgtct gctcgcacgg gttacgaagc ggcgtgattt ctgcgagtat
     2821 cgcgggagct gatggaatat ctcaagccta tcataggcct actgcgcgac tggctgccgg
     2881 tgctgatcac ggtgctggtc gcgatcgtag tggtttggct tgcgtactgg ttgctgttca
     2941 agcgccgcag tccgtcttcg gtgagccgca tctcgcagca gctcttcatc gctgcgctga
     3001 tcgggatctt cctggtcatc gtgatcctcg agctccccat cggggacgca ccgcgggggc
     3061 agttgctgag cctactcggg ctcctcgtca cggcggcgat cgcgctctca tctaccacgt
     3121 tgcttggcaa cgcgatggcg gggttgatgc tccgctcggt tcggaacttc aagcccgggg
     3181 acttcatcat cgtcgatagt catcgtgggc gtgtttcgga gctcggcttg ctccgaacgg
     3241 agattcagac cgacaaacga aacctcacga cactgccgaa tctgtatctg atcaacaatc
     3301 cagtgaccgt cgtccggccg tcagggacat tcatcgctgc gacggtatcg ctcggttatg
     3361 acgtgccgcg gcactcggtc gaggagtgtt tggtgaaggc ctctgagaac gcagggctca
     3421 cggacccgtt cgttttcgtg ctcgagctcg gcgacttttc gatcacgtac cagaccgcgg
     3481 ggttcctcga ggaagtgaag tacctgatca gcgcggaatc gaagctccgg gaatgtatgc
     3541 tggatgcgct tcacgaagcc ggcatcgaga tcgtttcgcc gacattcatg aaccagcgcc
     3601 agctttcgga agatcgacgg tttattccca ggaacgaacg ccacaccgtg gcgaagcccg
     3661 ctgccgaaga acctcgcccc gaagagctga tgttcgacaa ggccgaagtc gccgaaagcg
     3721 aagccaaggc caccgaacgg ctcaaggccg tggtcgagga gatggaaaaa attcaaaaaa
     3781 atgagattaa ggagctcagc aaagaggccg ccgccgcgcg gctcgaagag cttcagatcg
     3841 cccgttccaa gctcgagctc gagatcgagg cccgcaagga gcaaaaaaag gaagagccct
     3901 cggacgaggg cgcttagcgg cactccactt gagtggtcgt ctcccaccgc gtccgctcga
     3961 tctcggcgac cgcgactgcg tcgatgaatt gaacgcgggc catcgcggcc tgctgctcgc
     4021 ggaggttcac gaagatgagg ttgctcgagc cgaactcgaa tctacgccgt tcgcctttgg
     4081 ctagctcctc ggccgtgccc accacttgct gcgtgagctc gacgcgttcg tgcgcggcgc
     4141 ggacggcgga cgccgcatca cgcattctgg caaccaactg atcctcgagc aggtcgatac
     4201 ccgcccgact ggcggcgacc ttggcgcggg cggcgctcgc acggcctcgt tcgtcgcgaa
     4261 agagaagagg catcgagagc tctagaccgg cctggaacac gtttcctggg agggtgaaat
     4321 cgaggtcggt gcccgtgagc tcgccgaggt cgcgcgagta ctggaaccgt ccccgcagct
     4381 cgggggcgag ctggttccgc gtgagcttca cgtcgatctc cgccgcatcg agcttggccc
     4441 gcgcgctccc aagctcgggg tgacaggcaa gcacgcggct ctgcaccagg tcttcggcta
     4501 gctccggctc ggcagagcga acggcctcct tcggaagcct gctcgggtcg gggacgaccg
     4561 agcgacccgc agggtcccgg aggaacaagg aaagcccgaa ggcggcttgc tcgaaacttc
     4621 gctgcgcgga aaccaggaaa gcgcgacgct cgagcaggat gcgctcgtta tcggtgacgt
     4681 caaactccgc gatcgatccg gctgcaagcc gccgccgcag ttgcgtgtcc cgatcttcgg
     4741 cgagggcaag aagcgccttg gtgacctcga gcttctgtcc cgcgctcacc caactccagt
     4801 aggcccccgc tgccgctaac tcgaggttga gagctgcgct cgagaggtct tcttccgcgg
     4861 cttcttcgag gtggatcgcg cgagcccgct ccgcccgagg ctcatcgatg acgcctccgc
     4921 gccagatcgg gagctcgatc ccagcgcgga gctcgccgcc ggagagggtt tggtcggagc
     4981 ggtaggttgg ccagcgttga tcgatgctga cgccgacacg gtatcccacg tagatctccg
     5041 aaccccagag gctcgtcggt tgacgaagct ccgcgttggc ggaacgaaga tcatagtagg
     5101 cacccccggt gagcgaaaga tcgccatcga tcctggggtc gaaagcgccg cgcgcttcgg
     5161 tggtcacgcc cttggcacgg cggacctctt cgacggcacg ccgaatgcgc ggatcgtgtg
     5221 cggtgacgga gctcaagacc tcgtcaaatt gcagtggctg ggactgcgcc cgcgtcgcgt
     5281 tcaggagcag aaccgaagcg accgagccca tcaggatgcg tatccaacgc gtccgtggcg
     5341 tcatttggca ccctcgttct gacttgccat cggaggcggc gcggtcgtcg gtggaaagcc
     5401 gttgaactgc ctccagacct cgaagccaag acgcacgcga tccagtagaa tccaaccttt
     5461 ggcgaggacg ccttggcgca gatagctcgc ggcgggccat gggccgtcgt ttggatcagg
     5521 caccacgacg attcgaaaat cgccgcgccc gtcgtccgtc gcgtcgacga acgcgacccg
     5581 accaccgaac gtgcccacgg caaccgaggg ccatccggag aattgaaccg caggccagcc
     5641 ttcgaattgg aggcgaacct ctctcccggg cgtgatcaaa gccgcgtcgt tgccatcgac
     5701 gtaaagcgca accgcgcggt cctcggtggt cggcaccagg acggcgagca gctcgcctcg
     5761 gcttacctgc tcaccgccca ggcggccgac gactttgacg atcgtgccct cgatgggcgc
     5821 acggatgacc tggtcctgct gccgcgcgat gtccacttcc aagcgtgaga ggctaccgtt
     5881 ggcggaagcg acctccgtct ccgcgctgcg cagcccagcc tccgcgttct cgatctcggc
     5941 ttcggtggag gcgatcaccc gacgaagcgc cgcccggctc tgcgacaagc ggtttcgcgc
     6001 cgcgctcaga tcggcatttg cggaggcgac ttgcgccttg ctggtcctca cggccagctc
     6061 agcgagctcg agctcgcgct gtgagactag cccatcgcga ctgaggtccc tctgacgccg
     6121 aaggttgagc tcgttggtgt cgagctcggc ttgcgccgcg ccgaggtcct gttgtgcagc
     6181 tgccacgtct tgctgggaga tggcgatgct cgattcggct ccactgattt cggcgcgctg
     6241 cgaccgccga agcgactcga ctcgggaggt aagggtttcg agacgctggc cttgagcaat
     6301 gcgctgtact tcgatggccg cgcgctgtgc cccgagacgg tctaggattc gctcatcgtt
     6361 gtcttccagt cggacgatcg gatcgccctt gctcacggtg gaaccttcct cgacgaacca
     6421 ttcggcgaca acgcccgaaa cggacgcttc gatcgcttgc tcccgcttgt cgggcgcata
     6481 ggcaaccacc ctaccgcggc cgcgaacgtt ttgggtccat ggcgcaagaa gcgcggccag
     6541 cgtgaacaga acgaacgcgc tcaccagaag cttggcgacc gatgcggcac gttgcggcgc
     6601 cggcacctcg agaccagaag ggatctcatt ctgttcgaat cccgtccagt gctgggtctc
     6661 ttcgatcgct cgattgactt cggtcatgcc gcctctcctt gcggtttgat catctcggac
     6721 aggtcaatca ggcgatcggt gcgcgcgaga acctggggct cgtcggaaac caataacacg
     6781 gagacatcgc ggtcgaccaa tagggcgtcg agaacccgaa tgcgctccga gggccctagc
     6841 cgatcgagtg tccgatcgag gatgagaatc gacggtttgc cggcgagtgc gcgcgcgatg
     6901 gtcaacgcca ggccctggga tcgggtcagc ggcgcaccgt gcggcaggat cttggtatcg
     6961 agcccgagag ccgaatcctt cacccattca tcgagaccga cgcggcgcaa tgcgttccag
     7021 gcctgatcag cggcaagctc gggattcgcc gcgtgcagat tgtccgctat acaacctgga
     7081 aggatctcgg tgccccgaac gagcgtcacg cgcgaacgga catcggtgag ggcgagatcg
     7141 cgaacatcgt gcccgtcgag ctcgatggtg ccggatttgg gagcgttgag tccgaaaacg
     7201 gcgtctacga gactggtggt gacccgctcg gacgctccgc tgagtcctat ccactcaccc
     7261 gggcgaatct cgagcgaaac gggacccggt gcgctgccgt cttccgtctc gatctctacc
     7321 tgggtgagga ccaacctgcc ttgcgacccc tggtttggcg gtgggctctc gccatttccg
     7381 cgctcgaggg gaaggtcgaa gatgtctccg agcttgtcga ctgcagctac caggtcatag
     7441 aaaatctcga ccttgccgcc aagcttggcg atcgatgcga cgacggcggt gacgatgagc
     7501 tctgcggcga cgagctggcc gatcgaaagc tgccgctcga tcactagcaa gccaccgacc
     7561 gcgagcagcg caacgcttgc tacaacttcg atcgtgagtg cgccgatcag ctgtcgaaag
     7621 acgactcgga agtggcgctc tcgcttgtgt aggtacgagc gcgcgagatc ttgcgcccgc
     7681 tctttggcga actccccgcc acccgcgagc ttgaacgaaa gtgggtgacg agcgatttct
     7741 tcgagccagc ccgcgaccgc atacttcgct ttcgactctt cgacgctgct tcgcgtacct
     7801 ccgcgcccga ggggaatgag cacgatgacg gcgccgatga tcaagacgat atcgaacgcg
     7861 agcaacgcgg ggtggtagaa cgcgagcaca actaggccga cgagagcgat gaggagtgcg
     7921 tcgatgcctc cgagcagcag cgaagcgacg ctcttctgcg tcgtgaacac gtcgaagaac
     7981 cggttgagaa gctccgggcc gttggcggct tcaagcgcct tggcgctcac gcgcggtagt
     8041 cggccgctga tctctgcgac gatccgaacg aagagacgac gctgcagcat ttccacgatg
     8101 cgtacctgaa gcgcacggag gaccgccgaa ccgatcaggg caacagcaag gaggacagtc
     8161 aacacgatga gcggctgaat caaagagcca aaggcgaccg tgttgaccag ggattgaacc
     8221 gcaatcggta ccgcaagcga gaggagaccg agcgctacgc cgtagatgat gatcacgacg
     8281 atgtcctgcc ggtcgagctc caacagctgc cgggtgcggc gaagggggga cggacggcca
     8341 gcagccaagg attcttgatt cacgatgctc caagcatgat aattttttcc tgcttgtcaa
     8401 tacatgtttt ctgttttagg taatgagaga caggaaaatg tcgatgtcga ttgacccaag
     8461 caccccaccc tggactttcc ttagcaatca cgggcacatc ctgctctcga tcgcccgaga
     8521 tccgaacatc cgggtccggg agatcgcgcg ctccgtcgga atcaccgagc gggccgtgca
     8581 acgcattctc ggggaattag aggaggcagg cgtgatttcg cgcctccgtc agggccgaag
     8641 aacgcactac gaaatcgacc gaagccagcc cctgcggcac cccgtggagg ccgcccactc
     8701 ggtaggggag cttcttcgct tggctcgttc gcgacgctag atcgctcaac ctagctcgag
     8761 gcttacgcca atgacgagct ggtagtcgtt cttgtagacc gtgacgtcgg ggtccgacga
     8821 aggcgccgga tcctccgtcc taaagaagag gaatgcgatg tcgaggtcga tcacgctgtt
     8881 gagctcgatt gcgaggtcgg ccttgccggt gtggttggta ttgccgatcg tggtgaccac
     8941 gaggttggtg aaccaactca gcgtgagatc gatatcgccg gtgatgtcga agtccgcata
     9001 ggtgaagagc gggatgaaaa agtcgttctg tggattct
//
LOCUS       DASRZF010000147        13486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3192773, whole genome shotgun sequence.
ACCESSION   DASRZF010000147 DASRZF010000000
VERSION     DASRZF010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 13486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13486
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3192773"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..1120)
                     /gene="dmpG"
                     /locus_tag="VFG22_06705"
     CDS             complement(92..1120)
                     /gene="dmpG"
                     /locus_tag="VFG22_06705"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: protein motif:HMM:NF006049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008701 - 4-hydroxy-2-oxovalerate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HET6415967.1"
                     /translation="MNLEGKKVTLHDMSLRDGMHPQRHQLTPDQMIEVATALDDAGVP
                     LIEVTHGDGLGGASVNYGFSAATDEEYLRAVVPRMKNAKISALLLPGIGTVDHLRMAH
                     DCGVHTIRVATHCTEADVSEQHIVLGAKMKMDTVGFLMMAHMISPEELLEQAKLMQGY
                     GANCLYITDSAGYMLPDHVTARVGLLREKLDPAVEVGFHGHHNMHMGIANSLAAIEAG
                     AARVDGSVAGMGAGAGNTPLEVFVAVLHRMGVTTGVDMWKLMDVAEDIVYPLMTHKVR
                     ADRDSLTLGYAGVYSSFLLHAKRAAEKYGLKSTDILVELGRMKTVGGQEDMIEDVALD
                     MKKAGAAE"
     gene            complement(1117..2010)
                     /locus_tag="VFG22_06710"
     CDS             complement(1117..2010)
                     /locus_tag="VFG22_06710"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006157.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HET6415968.1"
                     /translation="MTKVKAAIIGSGNIGTDLLYKALRSEIIEPVWMVGIDPESEGLK
                     RAKELGLKTTHQGVDGLEPHIVDDKIQIAWDATSAYVHRSNNERVKKYGVKMVDLTPA
                     AIGPFCVPPVNLREMVGRGEQNVNMVTCGGQATIPMVAAVSRVQPVEYGEIIATVSSK
                     SAGPGTRKNIDEFTRTTAKGVEVVGGADRGKAIIIINPADPPLIMRDTIHCLTKDPPR
                     QAEILESAEQMIAEVQKYVPGYTLKNGPVFDGNRVSFFMEVEGLGDFLPKYAGNLDIM
                     TAAGLRTAELIAEEIQRAEAA"
     gene            complement(2026..2826)
                     /locus_tag="VFG22_06715"
     CDS             complement(2026..2826)
                     /locus_tag="VFG22_06715"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET6415969.1"
                     /translation="MDDATRRKKIEELGDELYAALRARKTLSPLTERWPEITIEDAYH
                     VSLRMVQRRLETDGEKIIGKKIGVTSRAVQEMLGVFQPDFGDLTDKMLYPNGGPMPIS
                     EQLIQPRAEGEIAFKLKKDLKGPGVSNQDVLDATDYVMACFEVVDSRVHDWRVKIQDT
                     VADNASCGLFVLGDEHADPRALDFPSLRMRVFKNGELLSEGMGSAALDSPVNCVSWLA
                     NTLSEFGIDLNAGDVVLSGSLVPLEPVVAGDEMRLEVQGIGTASVRFT"
     gene            complement(2829..3998)
                     /locus_tag="VFG22_06720"
     CDS             complement(2829..3998)
                     /locus_tag="VFG22_06720"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6415970.1"
                     /translation="MPTREDLIQRARNLAPTLQERSKKAAELRRLPDETIEDFHRLGF
                     FKVVQPKRYGGYEMDPSIIFDLQLELGRGCASSAWVYGVLNVHSWQLALFSPEAQDEV
                     WAENPRTLISSSYMPVAKTEWVDGGVKLSGRWSFSSGCDACDWVFLGGFVPSEEGQPP
                     DMRTFLVPRSDYEIIDNWHVSGLRGSGSKDVVVDGAFVPDHRMHKFSDGFRQQSPGNE
                     INSSPVYKYPFGQIHVRSVSTPAVAAALGALDSYIDFMKSKVSQATGGKAKDSFTNSV
                     VAAEATAVLDREVLTLRRNFEEMSSYLQRGEKIPLERRVHFRNDSARAVRVSTEVVEQ
                     LFISCGARAAFVDHPINRHFQDVHAIRLHHANGPDTPASNMGGVLFGQKNNDFFI"
     gene            4204..5076
                     /locus_tag="VFG22_06725"
     CDS             4204..5076
                     /locus_tag="VFG22_06725"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET6415971.1"
                     /translation="MPMPSGLKAIDLMMNIPDGDPSAWYEFIKPLLLDEESRNLFTMP
                     AQYMFKDIPEVGEQKDYVQYAIGEMDKWGIERAMLGISGPNSTNLAACKKHPQRFFPS
                     YGANPNNGMEEVRTIRRLHKEHGLKAVTGFPSGLCPQVPLNDKKYYPIYAACVDLDIP
                     ICICVGVPGPRIPMEPQKVEHIDEVCWFFPELKFVMRHGGEPWVDLAVKLMIKYPNLY
                     YSTSAFAPKHYPKAIIEYANTRGADKIMYAGYFPMGLSLERIFTDMQNVAFKEHVWPK
                     FLSENAARVFQLDS"
     gene            complement(5121..7220)
                     /gene="rlmKL"
                     /locus_tag="VFG22_06730"
     CDS             complement(5121..7220)
                     /gene="rlmKL"
                     /locus_tag="VFG22_06730"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: protein motif:HMM:NF008748.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HET6415972.1"
                     /translation="MLPCPKGCEEVLAQELRDLDIEVTDLRPTAVHVNGTLRDGYRMC
                     LWSRVASRVLLPLVEVDAENDTGLYDALCHVSWWEHFDASRTFAIGSSQSSKSSRPAH
                     YWVQRAKDAIVDGFRNHGGTRPSIDKKDPEIRLHLHIGEGHHELSLDFSGDGLHRRGY
                     RRSGGKAPLKENLAAAILYLADWPKAARQGLALMDPTCGSGTFLVEGALMATNCAPGL
                     LRERFGFENWLKHDAAAYDAERATARAARKADCPGRIYGCDASEKALSLVRENLAAAG
                     VDQHVSLAVQELSTVRKPAEVGILVCNPPYGHRLGDEGTLFLFYQALGDTLKRAFDGW
                     TAYVFAAHGGNLKHLGLRPVRRHVLYNGAIECRLVEIPVRGVTGDDQSRAPAWRKPSE
                     SAQMFANRIKKNKKKWARWAKRNDVECYRIYDGDIPEYHVAVDLYQSKAIVHVFQKER
                     DADDDRVKQRVQDVLLTLPEALGIEPSDMVVKVRRKHDQGDQYARIAQQDSDIIVSEG
                     PLRFVVNLEDRIDTGLFLDHRTVRAYAFERCRGQRMLNLFAYTCSVSAAAAVGGAKQT
                     TSVDLSNTYLDWGKRNYEANGLDPAKHRFIRDDATRWMARDRNSYDWIFINPPTFSRS
                     KMSKGDFNIHKDHGGLIDNAMRSLDKSGELLFTTHARAFDLDDSIQKRFRVEDATQRF
                     VPPDFIRYPFQAFILRK"
     gene            complement(7258..7836)
                     /locus_tag="VFG22_06735"
     CDS             complement(7258..7836)
                     /locus_tag="VFG22_06735"
                     /inference="COORDINATES: protein motif:HMM:NF036523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HET6415973.1"
                     /translation="MDDRARRIVETAVELAERDGYQAVRLRDVAASAQVALGTVYKRF
                     ASKEEILIAALEQESEKLVSKIGKKPVPGNGPRERVAFVFSSLTKGLLRRPNLAKALV
                     RSLASGDPNITERVASFHALITAMVMAAIRGEAAADQSEWGGDVDQRERTIASILQNV
                     WFASFVGWAGGLHEAPQLLEDVDRTSELLLPE"
     gene            complement(7839..8813)
                     /locus_tag="VFG22_06740"
     CDS             complement(7839..8813)
                     /locus_tag="VFG22_06740"
                     /inference="COORDINATES: protein motif:HMM:NF039286.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET6415974.1"
                     /translation="MSTTEEKEEELFPGYPRGWFVIGVSSELAVGEVKPIKYFDREMV
                     LYRSESGATIVHDAFCPHLGAHLGHGGKVVGESIQCPFHAWRFDETGKCVEVPYAKRI
                     PPRACVKNNPVQEHNGLIFMWNAHIEGEEPRWEIPDLPEYGSDGWTDWGSALIDIKTH
                     PKEIVENLADKGHFGPVHGTWANEFGNEFEDHIGIQRVKGVAYPRGGGEDHFELQSTY
                     YGPGYMLTRMQSVLPNIMLLCHTPVDKNRVHVRIGAMIDVSNVKKGKEEFEEGYRRNI
                     IVGFKEDIAIWENKKYRTRPILCDGDGDVGALRRWYQKFYMPQEKVGY"
     gene            complement(8844..10454)
                     /locus_tag="VFG22_06745"
     CDS             complement(8844..10454)
                     /locus_tag="VFG22_06745"
                     /inference="COORDINATES: protein motif:HMM:NF005863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HET6415975.1"
                     /translation="MEYNLADLYEAIADVVPDNIALASGDVRRTYAELDKRANRLAHY
                     FQSCGIGPGDHIGLHLFNGHEFVEAILALFKIRAVGINMNYRFVGPEVRYMIENADLE
                     GIVTQRRFLPIINEAMEGLDPLKSLIVIEDGSDAVAAQESIEYETALARGSEARDFAP
                     RSGEDLYIIYTGGTTGMPKGVMWRHEDLFFAGLQGGSPGGDPVESPEQLIEQAKEGWY
                     TVTMLPCAPFIHGAAQWTQWICHTTGGKLVLQHGPSFDAKRILSLVAEEEVSTISLVG
                     DAMAIPLVEELRTGNYDMSNLAVIASAGAILSGSVQDEIEALLPDVMVINSFGTSESG
                     DLGRAAGDEDSHEGRPSFYMGDEVTVIDENGNQLEPGSGQIGMVARSGRLPLGYYKDP
                     EKTAERFKEFKGRRWVVPGDFATIELDGRITFLGRGSKCINTGGEKVFPEEVEEALKA
                     HPEIIDALVVAVPDPRFGSKVAAVFNTRGNKQLSLDEVQQHCRKHIAGYKVPRQISIT
                     ETVSRMPSGKPDYPWAEEIARNEASAQG"
     gene            complement(10468..11616)
                     /locus_tag="VFG22_06750"
     CDS             complement(10468..11616)
                     /locus_tag="VFG22_06750"
                     /inference="COORDINATES: protein motif:HMM:NF006180.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HET6415976.1"
                     /translation="MAEPCAVIGIGQTKHRESRRDLSIPGLVRDAAIRALQDADMTWK
                     DIDSIVIGTAPDMFEGVMMPELWLSDALGASGKPIFRVHTAGSVGGSTAVVASHLIQG
                     GVHEKVLTLAFEKQSEANAMWALSPAIPFQPQLVAGAGGYFAPLMRSYIRRAEAHPDT
                     GCMVAVKDRQHGLLNPNAHLHLDQTLEQVKASPMLWDPIRYSETCPSSDGAVAMVLVN
                     EKNARRVKNPAWVKGTSVRTERTFFAGRDQVNPAAGKLCAKDVYDEAGIHNPLKEIDC
                     AEVYVPFSWFEPMWLENLGFAEEGEGWRMTMDGKTRIGGEIPWNMSGGVMCTNPIGAS
                     GMLRFAEAARQVRGTAERHQVDGAKVALGHAYGGGSQFFAMWIVSSQR"
     gene            complement(11609..12670)
                     /locus_tag="VFG22_06755"
     CDS             complement(11609..12670)
                     /locus_tag="VFG22_06755"
                     /inference="COORDINATES: protein motif:HMM:NF005924.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HET6415977.1"
                     /translation="MRDVAIVSFSQLPSVKAIDAADEPELVQPVTSDALKQVGFSQDD
                     IGFTCSGSTDYLPGRPFSFVAAVDGLKAWPPIRESHVEMDGAFALYEAWVRLLHGDID
                     TALVFSFGKSSLGPVSQILNLQNDPYYVQPLGLDAISAAALQAQAMIDAGKASERDFA
                     EVVARNRKNAIDNPNAHIKGDFSVDAMLEEPYLSSPLRKSFCPPASDSAAAIVLAVGD
                     VARKLVKRPAWIRGIDHRTEPHALGQRDLTVSPSTKLAAEKAGVARGRVDVAELHAPF
                     AHQEIILREAMGLADDVKVNPSGGALAANSLMTAGLIRFGEAATRILSGDAERAVAHT
                     TSGVCLQHNLVAVLEASHG"
     gene            complement(12670..>13486)
                     /locus_tag="VFG22_06760"
     CDS             complement(12670..>13486)
                     /locus_tag="VFG22_06760"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HET6415978.1"
                     /translation="YEQLEGVKTADGRVLCPPLEYDEEGEPTTGEFVPLRPSGTVKSW
                     SWVQEPRTGHPLDRAFAWALIQIDGADNAMVHIVDAEKAKQMQTGMRVRLRWAEQKNG
                     TIRDIECFEPISPILPGPVHIEYKVNPGRHYSAYLRGLQQGKLIGGRDPQLGKVYVPP
                     RAADPMTGEPTSEYVEVADEGVLTTFTIIRIPFEGQKLKPPYCFGAIVLDGADMPIYH
                     LISGVPHEDIRMGMRVRAKWKPPEEWETSLENILYFEPTGEPDAPFESYKEHL"
BASE COUNT         2565 a         4206 c         3929 g         2786 t
ORIGIN      
        1 actgccgctg gtgccccaaa gcgatttggg cgcgttcgcg cgaacccagt cccgcgccga
       61 tgcgcggaag tcatccagct cactcacgcg atcactccgc tgcaccggcc ttcttcatgt
      121 cgagcgcgac gtcttcgatc atgtcctctt gaccaccgac ggtcttcatc cgtccgagct
      181 cgacgagaat gtcggtcgac ttgagcccgt acttctcggc ggcacgcttc gcatgaagca
      241 aaaacgacga atagaccccc gcgtagccga gcgtcagcga atcacggtcc gcgcggactt
      301 tgtgcgtcat cagtgggtag acgatgtcct cggccacatc catcaacttc cacatgtcga
      361 ctccggttgt gacacccatc cgatggagca ccgctacgaa cacttcgagc ggcgtgttgc
      421 ccgcccctgc tcccataccc gcgaccgagc catcgactcg ggccgcaccc gcttcgatcg
      481 cggcgagcga attcgcgatc cccatgtgca tgttgtggtg cccgtggaaa ccaacctcca
      541 cggctgggtc gagcttttcg cgcagaagcc ctacccgggc ggtgacgtga tcgggaagca
      601 tgtaaccggc cgagtcggtg atgtagaggc agttcgcgcc atagccttgc atcagcttcg
      661 cctgctcgag gagctcttca ggcgaaatca tgtgggccat catcagaaag cccacggtgt
      721 ccatcttcat cttggcgccg agaacgatgt gctgctcgga gacgtcggct tcggtgcaat
      781 gagtcgcaac ccgaatcgta tgcaccccgc aatcgtgcgc cattcgcaga tggtcgaccg
      841 tgccgattcc aggaagcaag agcgccgaaa tcttcgcgtt cttcatgcga ggcacgacgg
      901 cacgaaggta ctcctcgtcc gtcgccgccg aaaaaccata gttgaccgat gcgccaccga
      961 gtccgtcgcc gtgcgtgacc tcgatgagcg gcactccggc gtcgtccagc gcggtcgcaa
     1021 cttcgatcat ttggtcaggc gtgagctgat ggcgctgcgg atgcatgccg tcacgcaagc
     1081 tcatgtcgtg aagcgttacc ttcttgcctt cgagattcat gctgcctccg cccgctggat
     1141 ttcctccgcg atcagctccg ccgtacgcag gcccgccgca gtcatgatgt cgagattgcc
     1201 agcgtacttc ggcagaaagt cgccaagacc ctcgacctcc atgaagaagg agactcgatt
     1261 cccatcgaat acggggccgt tcttgagcgt atagcccggc acgtactttt gaacctccgc
     1321 gatcatttgc tctgccgatt ccaagatctc tgcctgtcgc ggaggatcct tggtgagaca
     1381 gtgaatcgtg tcccgcatga tgagcggagg gtcggcagga ttgatgatga tgatcgcttt
     1441 tccacggtcc gcacctccta cgacctcgac tcctttcgcg gtcgtgcggg tgaactcgtc
     1501 gatgttcttg cgtgtccccg ggccagccga tttggaagaa accgtcgcga tgatctcgcc
     1561 gtattcgacg ggctgaaccc tgctgacggc agcgaccatg ggaatcgtgg cctgaccacc
     1621 gcaagtcacc atgttcacgt tttgctcgcc ccgtccgacc atctctcgaa gattgaccgg
     1681 aggcacacag aaggggccga tcgcagcggg cgttagatcg accatcttga cgccgtactt
     1741 cttgacgcgt tcgttgtttg atcggtgtac gtatgcgcta gtcgcatccc aagcgatctg
     1801 aatcttgtcg tcgacgatgt ggggttccag tccatcgacg ccttggtgcg tcgtcttcag
     1861 tccgagctcc ttcgctcgct tcagtccttc agactcggga tcgatgccaa ccatccacac
     1921 cggttcgatg atctcgctcc gaagtgcttt gtagagcaga tcggtgccga tgtttccaga
     1981 tccaatgatc gctgccttca ctttcgtcat ctgctcttcc ctatcctaag tgaatctcac
     2041 cgaggcggtt ccgattcctt gcacctcgag gcgcatctcg tcccccgcga cgacgggttc
     2101 gagtggcacg agagagccag agagcaccac gtcaccggcg ttgagatcaa tgccgaactc
     2161 cgacaaagtg ttggcgagcc aactcacgca gttgaccggg gagtcgagtg cggcggatcc
     2221 catgccctcg ctgaggagct caccgttttt gaagacgcgc atcctcaagc tggggaagtc
     2281 gagcgcccgc ggatctgcgt gctcatcacc gagcacgaac aaaccgcaac tcgcgttgtc
     2341 ggcgacggtg tcttggatct tgacgcgcca gtcgtggacg cggctgtcca cgacctcgaa
     2401 gcaggccatc acgtaatcgg tcgcgtcgag gacatcctgg ttgctgaccc cgggaccctt
     2461 gaggtctttc ttgagcttga atgcgatctc gccttcggca cgcggctgaa tcaattgctc
     2521 gctgatcggc atcggcccac cgttcggata gagcatttta tcggtgaggt ctccgaagtc
     2581 gggttggaac acgccgagca tttcctgcac cgcgcgactc gtaacgccga tcttcttgcc
     2641 gatgatcttc tctccatccg tctcgagacg gcgctgcacc atccgcaagg agacatgata
     2701 cgcgtcttct atcgtgatct cgggccaacg ctcggtgaga ggactcagcg tcttgcgggc
     2761 gcgcagcgcg gcgtagagct cgtcgccaag ctcctcgatc ttttttcgcc gcgtcgcatc
     2821 gtccatgcct agatgaagaa atcgttgttc ttctggccga agagcactcc gcccatgttc
     2881 gatgccgggg tatcaggacc gttggcgtgg tgaagtcgaa tcgcatgaac atcctgaaag
     2941 tggcggttga tgggatgatc cacgaatgca gcgcgagccc cacaagatat gaagagttgc
     3001 tccacgacct cggtcgaaac gcggaccgcg cgcgccgagt cgttgcggaa gtgaacgcgt
     3061 cgctcgagcg ggatcttttc gcctcgttgc agataggaag acatctcttc gaaattgcgt
     3121 cggagggtca agacctctcg gtcgagcacc gcggtcgcct ccgctgctac gacgctgttg
     3181 gtgaacgaat ccttcgcctt tccaccggtg gcttgcgaga ccttggactt catgaagtcg
     3241 atgtacgagt ccagcgcgcc gagcgctgct gcgaccgccg gggtcgagac cgaacgaacg
     3301 tggatttggc cgaacgggta cttgtagacg ggcgaggaat tgatctcgtt gcctgggctc
     3361 tgctgccgga atccatcgga aaacttgtgc attcgatgat ctggaacgaa ggccccgtcc
     3421 actaccacgt ccttgctccc cgaacctcga agcccggaaa cgtgccagtt gtcgatgatt
     3481 tcgtagtccg atctgggtac gaggaacgtg cgcatatccg gcggctggcc ctcttcgctg
     3541 ggcacgaacc ctccgagaaa gacccagtcg caagcatcgc agccgctcga gaacgaccaa
     3601 cggccgctga gcttcacccc gccatcgacc cattcggtct tggcaaccgg catgtacgag
     3661 gaagatatga gggtcctcgg gttctcggcc caaacctcgt cctgcgcctc tggcgagaag
     3721 agtgcgagct gccagctgtg gacgttcaat actccgtaga cccaggcgct cgatgcacag
     3781 ccgcggccga gctcgagttg caagtcgaag atgatcgaag gatccatctc gtacccgcca
     3841 taccgcttcg gctggacgac cttgaagaac ccgagccgat gaaagtcttc gatcgtctcg
     3901 tcgggaagcc ttctcagctc agcggctttc ttcgaacgtt cctgcagcgt cggtgcgaga
     3961 ttgcgggcgc gctgaatcaa gtcttctcgt gtcggcatcg ggctctgatc tccgtccatg
     4021 gggcatctcc gggctcggtc ggctttccgc cgccgattct tgatgggtca tctggtctcg
     4081 gctttacaga tgggccacct caggccataa agtagaacgt gttctagccc acgggccgag
     4141 acatgtccag cgggctcatg ccaacataga gcccagactg ggacccggaa aggacgcaat
     4201 ttcatgccga tgccgagtgg cctcaaagcc atcgatctga tgatgaacat ccccgacggg
     4261 gacccgagcg cctggtacga gttcatcaag ccgctcctgc tcgatgagga gagccgcaac
     4321 ctcttcacga tgccggccca gtacatgttc aaggacatcc cggaggttgg cgagcagaag
     4381 gactatgtcc agtacgcgat cggcgagatg gacaagtggg gcattgagcg cgcaatgctt
     4441 ggcatttcgg ggcccaactc gacgaacctc gccgcttgca agaagcaccc tcagcgtttc
     4501 ttcccatcgt acggggcgaa cccgaacaac ggaatggagg aggtccgaac gattcggagg
     4561 ctccacaagg agcacggcct gaaggctgtg acggggttcc cctccggcct ctgcccccag
     4621 gtcccactga acgacaagaa gtactacccc atttatgcgg cctgcgttga cctggacatc
     4681 ccgatttgca tctgtgtggg cgtcccaggc ccgaggatcc cgatggagcc tcagaaggtc
     4741 gagcacatcg acgaggtctg ctggttcttc ccggagctca agttcgtgat gcgccacgga
     4801 ggagaaccct gggtcgacct ggccgtgaag ctcatgatca agtacccgaa tctctactac
     4861 agcacgagcg cttttgcgcc gaagcactac ccgaaagcga tcatcgagta cgcgaataca
     4921 cgcggggccg acaagatcat gtacgccgga tactttccga tgggcctttc gctggaacga
     4981 atcttcacgg acatgcagaa cgtcgcgttc aaggagcacg tctggcccaa gttcctcagc
     5041 gagaacgccg cgcgcgtgtt ccagttggat agctgagctc tgagcatcgg ccaggtcggg
     5101 agcggccggt cctgcctccc tcatttacgg aggatgaagg cctggaacgg gtaacggatg
     5161 aagtcgggag ggacgaaacg ttgggttgca tcctcaactc gaaatcgctt ttgaatggaa
     5221 tcgtccaagt cgaaggcccg ggcgtgggtc gtgaacagga gctcgccgct cttgtcgagc
     5281 gaccgcatcg cgttgtcgat caagccccca tgatccttgt gaatgttgaa gtcccccttg
     5341 ctcattttgg atcgcgaaaa ggtcggagga ttgatgaaga tccagtcgta gctgttgcgg
     5401 tctctggcca tccagcgcgt ggcgtcgtcg cggatgaatc gatgtttcgc tggatccaat
     5461 ccgttggctt cgtagttccg tttgccccag tcgagataag tgttggatag gtccacgctg
     5521 gtggtttgct ttgcaccacc taccgcggcc gcagcgctga ccgaacacgt gtacgcaaag
     5581 agattgagca ttcgttggcc cctgcagcgc tcgaacgcgt atgcgcggac cgtgcggtga
     5641 tccaagaaaa ggcccgtatc gatgcgatcc tctagattga cgacgaagcg cagtggcccc
     5701 tcgctgacga tgatgtcgct gtcttgctgt gcgatgcgcg cgtactgatc gccctggtca
     5761 tgtttccgac ggactttcac gaccatgtca ctcggctcaa tcccgagcgc ttcggggagt
     5821 gtcagcaaga cgtcttggac ccgctgcttg acgcggtcat catccgcgtc ccgctccttc
     5881 tggaatacgt gaacgattgc cttcgactga tagagatcca ctgcgacgtg gtactcgggg
     5941 atgtctccat catagatacg gtaacactcc acatcgtttc gcttcgccca gcgcgcccat
     6001 ttcttcttgt tcttcttgat ccgattggcg aacatctgag cactctcgct cggttttcgc
     6061 catgccgggg cccgggactg gtcgtcgcca gtcaccccgc gaacgggaat ttccacgagc
     6121 cgacactcga tggcgccgtt gtagagcacg tgacggcgga cgggccggag cccgaggtgc
     6181 ttcagattgc cgccgtgggc cgcaaagacg taggcagtcc atccatcaaa ggcgcgcttc
     6241 agagtgtccc ctaacgcttg atagaagaga aacagggtgc cttcatcacc gaggcgatgg
     6301 ccgtacggag ggttgcagac gagaatgccg acctcggctg gcttccggac cgtggacagc
     6361 tcttggacgg caagcgagac gtgctgatcg acgcctgccg cggccaagtt ttcgcgaacc
     6421 aaggagagcg ccttctcgga ggcgtcgcaa ccgtagattc gacctgggca gtcagccttg
     6481 cgcgcagcgc gtgcagtcgc tcgttctgcg tcgtacgccg cggcgtcgtg cttcagccag
     6541 ttctcgaacc caaagcgctc tcggaggagc ccgggtgcgc aattcgtcgc catgagagcg
     6601 ccttcgacca agaaagtccc cgaaccgcag gtggggtcca tcagtgcaag accctgccgc
     6661 gccgccttgg gccaatcggc gagatagagg atcgcagcgg cgaggttctc tttgagcggc
     6721 gccttccccc cggacctgcg gtagccgcgc cggtgcaatc catcccccga gaaatcgagc
     6781 gaaagctcgt ggtgcccctc accgatgtga aggtgaagtc ggatctccgg atccttctta
     6841 tcgatgctgg ggcgtgtgcc gccatgattg cgaaacccat cgacaatcgc gtcctttgcg
     6901 cgctgcaccc aatagtgcgc cggccgcgag ctcttggatg actgcgagct cccgatcgcg
     6961 aacgtccgcg acgcgtcgaa gtgctcccac cacgagacgt ggcagagcgc gtcatagagc
     7021 ccggtgtcgt tctctgcatc gacctcgacg agcggaagca agacacgcga tgccacccgg
     7081 gaccagaggc acattcgata accgtcgcga agtgtgccat tcacgtgcac tgccgtaggc
     7141 cggaggtcgg tcacctcgat gtcgagatcg cgcaattcct gagcgagcac ctcctcgcaa
     7201 cccttggggc aaggaagcac ccacctgaat cgccgcgcag cgtcgatgcg tccccgccta
     7261 ctctggcaac aggagctcgc tcgtcctgtc gacgtcctcg agcagctgtg gggcctcgtg
     7321 caaaccgccg gcccaaccta cgaacgatgc aaaccaaacg ttctggagaa tcgacgcgat
     7381 ggtccgctcg cgctggtcga cgtccccgcc ccactcgctt tgatccgccg cggcttcacc
     7441 gcgaatcgct gccatgacca tggccgtgat gagcgcatgg aagcttgcga cacgttcggt
     7501 gatgttggga tcgcccgatg cgagcgatcg gaccagagct ttggcgaggt tcggccggcg
     7561 caagagtcct ttcgtcaacg aagagaaaac aaaggctacg cgctctcttg gtccattgcc
     7621 gggaacgggc ttctttccga tcttcgaaac cagcttctcg ctttcctgct ccagcgcagc
     7681 gatgagaatt tcctctttgc tcgcgaagcg cttgtagacg gtgccgagcg caacctgtgc
     7741 acttgccgcg acgtctcgca gacgaaccgc ttgataccca tctcgctccg cgagctcgac
     7801 ggcggtttcg acgattcgcc tggctcgatc atccatgact agtatccaac cttctcttgc
     7861 ggcatgtaga acttctgata ccagcggcgc aacgctccaa catcgccgtc accatcgcag
     7921 agaatcggtc gcgtccgata ctttttattc tcccaaatgg cgatatcttc cttgaaaccg
     7981 acgatgatgt ttcggcggta gccctcctcg aactcttcct tacccttttt cacgttcgaa
     8041 acgtcgatca ttgcgccgat tcgaacgtga acgcggttct tgtccactgg agtgtgacag
     8101 aggagcatga tgttcggcag aacactttgc atcctggtga gcatgtagcc agggccgtag
     8161 tacgtggact gaagctcgaa gtggtcctca ccgcccccgc gtggatacgc gacccctttc
     8221 acgcgttgaa tcccgatgtg gtcctcgaac tcattgccga actcattcgc ccaggtcccg
     8281 tgcaccgggc cgaagtgtcc cttgtcggcg agattctcga cgatttcttt cgggtgggtc
     8341 ttgatgtcga tcagtgccga tccccagtcg gtccagccat cgctcccgta ctcgggaaga
     8401 tcggggatct cccaacgggg ctcctcccct tcgatgtgcg cattccacat gaagattagg
     8461 ccgttgtgct cctgcacggg attgttcttc acgcatgcgc gcggtgggat ccgtttcgca
     8521 tagggcactt cgacgcactt gccggtctcg tcgaaccgcc acgcatggaa cggacattgg
     8581 atcgactcgc cgaccacttt gcccccatgc ccgagatgcg ccccgaggtg tggacaaaac
     8641 gcgtcgtgca cgatcgtcgc accactctca ctgcgataca aaaccatctc gcgatcgaag
     8701 tacttgatgg gtttgacctc gccgactgcc agctcgctcg agactccgat gacgaaccaa
     8761 ccacgcgggt acccggggaa aagctcctcc tctttttctt ctgtggtcga catgggttcc
     8821 tctcgattct ccgtggtgcg agctcagcct tgagcgctgg cctcattgcg cgcgatctct
     8881 tcggcccatg gatagtcggg cttgccactc ggcatgcggg aaaccgtttc agtgatggaa
     8941 atctgtcgcg ggactttgta gcccgcgatg tgcttgcggc aatgctgttg gacttcgtcg
     9001 agcgagagct gcttgtttcc tcgggtgttg aagacggcgg caaccttcga gccgaatcgc
     9061 gggtccggaa cggcaactac gagcgcatcg atgatctcag gatgcgcctt gagagcctcc
     9121 tccacctctt ccgggaacac tttttcgcca cccgtattga tgcacttgct gccgcggccg
     9181 aggaaagtga tgcgaccgtc gagctcgatg gtggcgaagt cgccgggcac gacccaacgc
     9241 cgtcccttga actccttgaa gcgctcagcg gttttttcag ggtctttgta gtacccgagt
     9301 ggcaagcggc ccgatcgagc gaccatgcca atctgccctg agccgggctc gagctggttc
     9361 ccgttctcgt cgatcaccgt gacctcgtca cccatgtaga acgacggacg cccttcgtgg
     9421 ctgtcttcgt cgccggcggc gcgaccgagg tcacccgact cactggtacc aaagctgttt
     9481 atcaccatga cgtccggcag aagcgcctcg atttcgtctt ggacgctccc agaaaggatc
     9541 gcgcccgcag aggcgatcac cgccaagttc gacatgtcgt agttgccggt ccgtagctct
     9601 tccaccaagg gtatcgccat cgcatctccc acgagcgaga tggtcgaaac ctcttcctcg
     9661 gcgaccagcg acagaatacg tttcgcgtcg aagctcgggc cgtgctggag cacgagcttc
     9721 ccgccggtcg tatggcaaat ccactgggtc cactgcgccg caccgtgaat gaacggagca
     9781 cagggaagca tggtgaccgt gtaccaaccc tctttggcct gctcgatcag ctgctcggga
     9841 gactcgaccg ggtccccgcc cggcgacccg ccttgcagtc ctgcgaagaa aaggtcctcg
     9901 tgccgccaca tcacaccctt gggcatcccc gtcgtgccac ctgtatagat gatgtagagg
     9961 tcctcgccgg atcgcggcgc gaagtcacgc gcctccgaac ctcgtgccag cgcggtttcg
    10021 tactcgatcg actcctgcgc agccaccgca tccgagccgt cttcgatcac gatgagcgac
    10081 ttgagagggt cgagaccttc catcgcctcg ttgatgatcg gaagaaagcg gcgttgagtg
    10141 actattccct cgaggtccgc gttctcaatc atatatcgaa cttcgggccc tacgaacctg
    10201 tagttcatgt tgatgcccac cgcgcggatc ttgaagagcg cgagaatcgc ctcgacgaac
    10261 tcgtgcccat tgaagaggtg gaggcctatg tggtcgcccg gaccgatgcc gcaggactgg
    10321 aagtagtgag cgagccggtt ggctcgctta tctagctctg cgtaagtgcg acggacgtcc
    10381 cctgaagcca aggcgatgtt gtccggcacg acgtccgcga tggcttcgta aagatctgca
    10441 agattgtatt ccacttggtc tctcctgcta gcgctgcgag ctgacgatcc acatcgcgaa
    10501 gaactgcgag ccacctccgt aggcatgacc gagggcgacc ttggcaccgt cgacctgatg
    10561 ccgttcggca gttcctcgca cctgccgggc cgcttccgcg aaccggagca tgccactggc
    10621 gccaatcgga ttggtgcaca tcacaccacc cgacatgttc cacggtatct cgccaccgat
    10681 cctcgtcttt ccgtccatcg tcatgcgcca gccctcgcct tcttcggcga agcccaggtt
    10741 ctcgagccac atcggctcga accacgaaaa cggaacgtac acctcggcgc agtcgatctc
    10801 cttgagcggg ttgtgaattc cggcttcgtc gtagacgtcc ttggcgcata gctttccggc
    10861 ggcggggttc acctggtcgc gtcccgcaaa gaacgtccgc tccgttcgaa ccgacgttcc
    10921 cttgacccaa gcgggattct tcacgcgtcg ggcattcttt tcattgacga gcaccatcgc
    10981 aacggcgcca tcggacgatg ggcaagtctc ggaatagcgg atgggatccc aaagcatcgg
    11041 ggaagccttc acttgctcca gggtctgatc gaggtgcaga tgggcgttcg ggttgagcaa
    11101 gccatgctgg cgatctttca ccgcgaccat gcacccggtg tccggatgcg cttccgcacg
    11161 acggatgtac gaacgcatca gaggtgcgaa gtagcctcca gcgccggcga cgagttgagg
    11221 ctgaaagggg atcgccggtg aaagcgccca catcgcgttg gcctcgctct gcttctcgaa
    11281 cgcgagcgtg aggacctttt cgtgcacccc accttggatc aggtgcgatg caaccaccgc
    11341 agtcgagcca cccaccgagc cggcggtatg cacccggaag atgggcttcc cggaagcacc
    11401 gagagcgtcc gaaagccaga gctccggcat catgacgcct tcgaacatgt cgggagcggt
    11461 gccaatgacg atggagtcga tatccttcca agtcatgtcc gcgtcttgca acgctcgaat
    11521 agccgcatct cgaacgagac ctgggatcga cagatcgcgg cggctctcgc gatgtttggt
    11581 ctgtccgatc ccgatgaccg cgcacggctc agccatggct cgcctccaaa actgcaacga
    11641 ggttgtgctg aaggcagacg ccgctcgtcg tgtgcgcgac ggcgcgctcc gcatctcccg
    11701 agaggatccg tgttgcggct tcgccgaatc gaatcagtcc tgcggtcatg agcgagttgg
    11761 ccgccaacgc accgccggat gggttgacct tcacatcgtc cgcgaggccc atggcctctc
    11821 gcaggatgat ctcctggtgc gcgaacggcg cgtgaagctc agccacgtcg accctgcccc
    11881 tcgcgacacc agccttttcg gccgcgagct tcgtggacgg cgagacagtc aggtcgcgct
    11941 ggccgagcgc atggggctcg gtcctatggt cgataccgcg aatccatgct ggccgcttca
    12001 cgagcttcct cgcaacatct cctaccgcca ggacaatcgc cgcagcggaa tccgacgcag
    12061 gagggcagaa gctcttgcgc aacggtgagc tgaggtacgg ctcttcgagc atcgcgtcga
    12121 cgctgaaatc gcctttgatg tgggcgttcg gattgtcgat cgcgttctta cgattgcgtg
    12181 cgacgacttc tgcgaagtca cgctcgctcg ccttgcccgc gtcgatcatc gcctgcgcct
    12241 gaagcgccgc agcgctgatc gcgtcgagtc caagtggctg aacgtagtag ggatcgttct
    12301 gcaagttcaa gatctgagac accgggccaa gtgagctctt tccgaacgaa aacacgagcg
    12361 cggtatcgat atctccatgc aggagacgaa cccacgcctc gtagagcgca aaggccccgt
    12421 ccatctcgac gtgagactca cgaatggggg gccaggcctt gagaccatcg accgcggcca
    12481 cgaaagagaa cggcctccca ggaaggtagt cggtgcttcc ggagcaagtg aatccaatgt
    12541 cgtcttggct gaaaccaact tgtttcaacg catccgatgt gacgggctgg acgagctccg
    12601 gctcatcggc tgcgtcgatg gccttgaccg atggaagctg cgaaaaagaa acgatggcta
    12661 cgtcgcgcac tagagatgct ccttgtaact ctcgaaggga gcgtccggct cgcccgtggg
    12721 ctcgaagtac agaatgtttt cgagcgaagt ctcccactcc tctggaggct tccacttggc
    12781 cctcacgcgc attcccattc gaatgtcttc gtgtggaaca ccgctgatca aatggtaaat
    12841 cggcatatcg gccccatcga ggacgatcgc gccgaagcaa tatggcggtt tgagcttctg
    12901 gccttcgaag gggatgcgga tgatcgtgaa cgtggtgagc acaccttcgt ctgcgacctc
    12961 gacgtactcg ctggtcggct cgccggtcat ggggtccgcg gctcgcggcg gcacgtagac
    13021 tttcccgagt tgcggatcgc gaccgccgat cagcttaccc tgctgcaacc ctcggagata
    13081 cgcactgtag tgccgtccag ggttcacctt gtattcgatg tgtaccggac cgggcaagat
    13141 gggggaaatc ggctcgaagc attcgatgtc acgtatcgtg ccgttctttt gttcggccca
    13201 acgcaaacgc acgcgcatcc cggtctgcat ctgcttggcc ttctcggcat cgacgatgtg
    13261 aaccatggcg ttgtcggctc cgtcgatctg gatgagagcc caagcaaacg cacgatcgag
    13321 gggatgcccg gtgcgaggct cttgtaccca ggaccaggac ttgaccgtcc cggacggacg
    13381 caaggggacg aactcgcccg tcgtgggctc accctcctcg tcgtattcga gcggagggca
    13441 gagcacgcga ccgtcggctg ttttcacgcc ttcgagttgc tcgtac
//
LOCUS       DASRZF010000148         4419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1475925, whole genome shotgun sequence.
ACCESSION   DASRZF010000148 DASRZF010000000
VERSION     DASRZF010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1475925"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="VFG22_06765"
     CDS             <1..478
                     /locus_tag="VFG22_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415979.1"
                     /translation="QDSFAHTIRSDATALRTIVHVLNYVDAIYKDFNESRDGIAHSRH
                     LDKCDEADVVALRQAADLSMQDLLAAFLQTRAGDSTAIDDLLDDWLTLEEGCNVENDF
                     CGNASEVATARQDPTDPILPEWMTCSARVEAVPGTWETVAGMFLVLALAARRSRRT"
     gene            475..1398
                     /locus_tag="VFG22_06770"
     CDS             475..1398
                     /locus_tag="VFG22_06770"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET6415980.1"
                     /translation="MTRSGAAILCIALSLGAQTALADGEPGLAAPDVEQETPSHPEAE
                     RSYRLEWHYKRVHWAEGVTAGVLGVAAFAIEFSPIRSEPRWTRNNAFDDWFQDRLIAP
                     SGKQQKYAKASDALAATSIAFPVLVDLIGMTLIGDRNKDVGLQMFAIQAQAFAITGFV
                     TSLVKLSGRERPCAQSDDSASGSTDCNDPNRSYFSAHTSLAFAGAGLTCVEHQHLEFF
                     GRVGSPITCASALALATTTGVFRVVSNRHWMSDVITGAGVGLLAGWLMPWLMHYRHDL
                     SKREQSKMRHLYYLAPYGDAGSIGLSAAGAF"
     gene            complement(1401..2513)
                     /locus_tag="VFG22_06775"
     CDS             complement(1401..2513)
                     /locus_tag="VFG22_06775"
                     /inference="COORDINATES: protein motif:HMM:NF018880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415981.1"
                     /translation="MKLSLIRFVILLGLASGLIACSSDTDSTTPPSSYRDVGPWETGV
                     TTLMLADRMVEVWYPVRPGDEGDREQDAYFIRDALPDVFDALLPEDVNPPFLTDAYRD
                     APASKAGPFPLVLFAHGAASYRNQSTFLTTHLASWGFVVASVDYLERGLASVLGAQPD
                     PRIEDTELTRMVVALLASENEREGGLLQGRVATDRIAITGHSAGGGTAIRFGGEPDVV
                     TYIPISAGLSSDSTVTLPDQPSLWLAGSIDAVIEADRTAETYAQASAPARFVLIENMG
                     HLGPSDICAIGDSGGGVVQIALDAGLPLSDNLVRLGTDGCQADALPVEDGWPTIRHFV
                     TAQLRWAFGIDEEPIGLSQDVAEYLPEATFTYEESL"
     gene            complement(2582..3931)
                     /gene="rmuC"
                     /locus_tag="VFG22_06780"
     CDS             complement(2582..3931)
                     /gene="rmuC"
                     /locus_tag="VFG22_06780"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HET6415982.1"
                     /translation="MMDPFLVAVAILLVINLIVTLVLLKRGVGDSRSREQAEAEDRAE
                     TARLLREELRAAREEASAQASELRQEVSRTHQASNETLVKTIAQIGESQTTKFQAFTV
                     QLKSLTDSNQTGLKHLQETVDGRLQELRSSNEQKLDQMREVVDEKLQSTLEKRLGESF
                     KLVGDRLEAVQKGLGEMRSLASGVGDLKKVLTNVKTRGTWGEVQLGALLEQILTPDQF
                     DRNVKPHPDSRDVVEFAIRLPGSSNEPEQCVWLPIDSKFPQEDYLRLVEASDNGDAPA
                     VQQAHGALVRSIQDSAKDVSAKYLNPPFTTDFAILFLPTEGLYSEVLRHPGLVERLQL
                     DHRIVVAGPTTLAAVLSSLRIGFRTLAIEQRSSEVWKVLGAVKTEFSKFGDVLDKVKR
                     QLETAGRTLDETGRRSRVMERKLRSVEELPAGTATGLLELADDAPVNLLETTEDGAA"
     gene            4039..>4419
                     /locus_tag="VFG22_06785"
     CDS             4039..>4419
                     /locus_tag="VFG22_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415983.1"
                     /translation="MPKGGTPEEELKALLPASIAVRFDEMFPAVGNGRVRRRDRKRAE
                     MLRKAEPVLQRVLNPMEVVRFATNGVRQLTWWLLTAGSMNPFANRTTLVLTDRRVLLI
                     HTDSKQRPRMFANQLPLERIRSTSG"
BASE COUNT          803 a         1436 c         1391 g          789 t
ORIGIN      
        1 ccaagacagc ttcgctcaca cgattcgctc cgatgccact gccctgagaa cgatcgtgca
       61 cgttctcaac tacgtcgacg ccatctacaa agacttcaac gagtcgcgcg acgggattgc
      121 tcactcgcgt catctcgaca agtgcgatga ggccgacgtc gtcgctctgc ggcaagccgc
      181 tgacttgtcc atgcaagact tgctcgccgc atttcttcaa acacgagcgg gggactcgac
      241 ggcaatcgac gacttgctcg acgattggct cacgctcgag gagggttgca acgtcgagaa
      301 cgatttttgc ggcaacgcgt ccgaagtcgc gacggctcgc caagacccaa ccgaccccat
      361 tcttcctgag tggatgactt gctcagcgcg cgtcgaagcg gtcccgggca cctgggaaac
      421 ggtcgcgggg atgttcttgg tgctggcgct cgcggcgaga cgaagcaggc gcacgtgacc
      481 cgaagcggcg ctgccatctt gtgcatcgcc ttgtcgttgg gcgcgcaaac cgccctcgca
      541 gacggcgagc cgggtctcgc tgcgccggac gtcgagcagg agaccccttc gcatcccgaa
      601 gccgaacgaa gctatcgact cgagtggcac tacaagcgcg tgcactgggc ggagggcgtg
      661 accgcgggcg tgctcggggt cgcggccttc gccatcgagt tttcaccgat ccgctccgag
      721 ccccgttgga ccagaaacaa tgccttcgac gattggtttc aggaccgcct catcgcccct
      781 agcgggaaac agcagaaata cgccaaggcc agcgacgcgc tcgcggcaac gagcatcgcg
      841 ttcccggtcc tggtcgacct catcggcatg accttgatcg gcgaccggaa caaagacgtg
      901 ggccttcaga tgtttgcgat ccaagcacag gcgtttgcga ttacgggctt cgtcacgagc
      961 ctcgtgaagt tgagcgggcg agaacgtcca tgcgcgcaat cggacgactc cgcatcaggc
     1021 agcaccgatt gtaatgaccc caatcgatcc tacttcagcg cgcacacctc gctggcgttc
     1081 gccggcgccg gcctcacctg tgtggagcat caacatctcg agttcttcgg ccgggtcggt
     1141 agcccgatca cctgcgcgag cgcgctggcc ttggcaacca cgacgggggt gttccgcgtc
     1201 gtgtcgaacc gccactggat gagcgacgtc atcaccggcg caggggtcgg tctgctcgcg
     1261 ggttggctca tgccctggct gatgcactac cggcacgacc tcagcaagcg agagcagagc
     1321 aagatgcgcc acctctacta cctcgcgccc tacggcgacg cgggctccat cggtttgagc
     1381 gccgccggcg cgttctagcg ctagagcgac tcttcgtacg tgaacgtcgc ctcgggaaga
     1441 tactcggcga catcttgcga aaggccgata ggctcttcat cgatgccgaa ggcccagcgg
     1501 agctgcgctg tgacgaagtg gcgaatggtg ggccagccgt cttcaacagg aagtgcatcg
     1561 gcctggcagc cgtccgtgcc aagtcgcacg aggttatcgg aaagcggaag gcccgcgtcg
     1621 agcgcgatct gcacgacgcc gccgccggaa tcgccgatcg cgcagatgtc gctcggaccg
     1681 aggtgcccca tgttctcgat gagaacgaag cgcgccggcg cactggcttg tgcgtacgtc
     1741 tcggccgttc gatcggcttc gatcaccgcg tcgatgcttc ctgcgagcca aagcgacggc
     1801 tggtctggca gcgtcaccgt gctgtcgctc gagagccctg ccgagatcgg gatgtacgtc
     1861 acgacgtcgg gctcgccgcc aaagcgaatc gcggtgcctc cgccggccga gtgcccggtg
     1921 atggcgatcc ggtcggttgc cacacggcct tgtagcaaac cgccctcgcg ctcgttctca
     1981 gatgcgagca gcgccacgac catccgcgtg agctccgtgt cttcgatcct gggatcgggc
     2041 tgtgcaccga gcaccgaggc cagcccgcgc tcgaggtagt cgaccgacgc cacgacgaaa
     2101 ccccaggacg caagatgcgt cgtgaggaag gtcgactggt ttcggtagct cgcggcaccg
     2161 tgcgcgaaga gcaccaaggg gaatggcccg gccttgctcg ccggcgcatc gcggtacgca
     2221 tcggtgagga acggagggtt cacgtcctcg ggaagtaggg catcgaaaac atcgggaagg
     2281 gcgtcccgaa tgaagtaagc gtcctgctcg cggtcgccct catcaccggg cctcaccggg
     2341 taccagacct cgaccatgcg gtcggccagc atcagcgtcg tgacgccggt ttcccaaggg
     2401 ccgacgtcgc gatagctcga cggaggggtc gtgctgtccg tatcggacga acaagcgatc
     2461 agcccagatg ccaagccgag aagaatgaca aatcgaataa gtgatagttt cataagcgct
     2521 ccaggccggg gcgtgtagca cagcaaaccg tgaccccaaa aggcgcggag ggcacccgcc
     2581 ctcaggccgc gccgtcttcc gtggtttcga gcaggttgac aggcgcatcg tccgcgagct
     2641 cgagcagccc cgtggccgta ccggccggga gctcttctac ggagcggagc ttgcgctcca
     2701 tgactcggga gcgacggccg gtttcgtcga gcgtgcggcc tgcggtttcg agctggcgct
     2761 tcaccttgtc gagcacgtcg ccaaacttgc tgaactcggt cttcacggcg ccgagcacct
     2821 tccagacttc gctcgagcgc tgctcgatgg ccagcgtgcg gaagccgatg cgcaggctgc
     2881 tcagcaccgc agccaacgtg gtcggcccag cgacgacgat ccgatggtcc aactgcaggc
     2941 gctccaccaa cccaggatga cgaagcacct cggagtagag cccctcggtc ggaagaaaga
     3001 gaatcgcgaa gtcggtggtg aatggcgggt tcaggtactt cgcgctcacg tccttcgcgg
     3061 aatcctggat cgaacgaacg agcgcgccgt gcgcttgctg gaccgcgggc gcgtcgccgt
     3121 tgtcgctggc ctccacgagg cgcaagtagt cctcttgagg aaacttcgag tcgatgggaa
     3181 gccacacgca ctgctcgggc tcgttgctcg aacccggaag ccgaatcgca aactcgacca
     3241 cgtcacggga gtccggatgc ggcttcacgt tccggtcgaa ctgatcgggc gtgaggatct
     3301 gctcgagcaa ggcgccgagc tgcacctctc cccaggtccc gcgcgtcttt acgttcgtga
     3361 gcactttctt gaggtcgccg acgccgctgg ccaggcttcg catctcgccg agcccctttt
     3421 gcaccgcttc gagccgatcg ccgacgagct tgaacgattc gccgagccgt ttctcgagcg
     3481 tgctctgcag cttctcgtcg acgacttcgc gcatctgatc gagcttttgc tcgttgctgc
     3541 tgcggagctc ttgaaggcgg ccgtcgacgg tctcctgcaa gtgtttgagc cctgtctgat
     3601 tcgaatcggt gagcgacttg agctgcacgg tgaacgcctg gaacttcgtg gtttgcgact
     3661 cgccgatttg agcgatcgtc ttgaccaggg tctcgttgct cgcctggtga gtgcgggaga
     3721 cctcttggcg gagctcgctc gcctgggccg acgcctcctc gcgtgccgcg cgaagctcct
     3781 cccgaagaag gcgagccgtt tcagcgcggt cctccgcctc ggcctgctcc cttgaccggg
     3841 aatccccgac cccccgcttg agaagcacga gtgtcacgat gagattgatg accaagagaa
     3901 tcgctacggc caccaagaac ggatccatca tcgagagctc ccccaagaac gcgaacgagg
     3961 ggcgatacta gcaccatcct gacgagctga gcagcgccga atgagggcgg ctcgcacgtg
     4021 ctaggctacg cgctgccgat gcctaagggg ggtacaccag aagaggagct caaagcgctc
     4081 ctgccagcgt caatcgcggt ccgcttcgac gagatgttcc cggcggtcgg caatgggcgg
     4141 gtccgccgcc gggatcgcaa gcgcgctgaa atgctgcgga aagccgagcc ggtcctccaa
     4201 cgcgtgctca atccgatgga ggtcgtgcgc ttcgccacca atggtgttcg tcaactcacc
     4261 tggtggctgc tcacggccgg ttcgatgaat ccttttgcca atcgcaccac gctcgtcctc
     4321 accgatcggc gtgtgctctt gatccacacc gattcgaagc agcgtccgcg catgttcgcc
     4381 aatcagctcc cgctcgagcg cattcgctcg acctcgggc
//
LOCUS       DASRZF010000149         4189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2164961, whole genome shotgun sequence.
ACCESSION   DASRZF010000149 DASRZF010000000
VERSION     DASRZF010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2164961"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VFG22_06790"
     CDS             complement(<1..222)
                     /locus_tag="VFG22_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415984.1"
                     /translation="MRRLRAAPVFMLVFFLAVSARAESRRTVAVKVVEIAGGRAYLAP
                     GQAAGVGLGSVVVFGSRRYRVVGATDSHAV"
     gene            complement(219..1133)
                     /locus_tag="VFG22_06795"
     CDS             complement(219..1133)
                     /locus_tag="VFG22_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HET6415985.1"
                     /translation="MRSGLLFLIGVAALAGLAGIAVAKDPKPKAAPATAPNHNSITEG
                     IDCGNCHTPAGWKMSTGKAGQGFDHSRTGFPLTGRHESAACNQCHSGEKRVTRECENC
                     HSDPHQSRMSGRCASCHSSRRWDDMEPFEIHRRTRFPLTGMHALAACTECHLRNNERQ
                     WTTVQSDCFSCHASDFLDPNIHPSHRGTATSAPFPQDCSQCHVASAWSPAFIDPSAIA
                     SSSTASLTGLGGMVVPANHDRHFPVSHGPHRGAPCNSCHIAAQTPRLVRCTGCHAHNA
                     VKLQQQHGRRVSSSGGACLGCHPGGTAR"
     gene            complement(1130..2767)
                     /locus_tag="VFG22_06800"
     CDS             complement(1130..2767)
                     /locus_tag="VFG22_06800"
                     /inference="COORDINATES: protein motif:HMM:NF014176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HET6415986.1"
                     /translation="MSGRIKALVIVGPLVIAAAIAGALVPRSSGAQFLSPGKLTTAHA
                     EIDGDANCNQCHSTGKRIDANACLGCHDDLGSRIRRQAGLHGREYRGQDCGGCHVEHL
                     GRNASLVRWPGGTPDRFDHGQSGWPLEGAHRSVRCQDCHKVRNRRGNATYLGLRTECA
                     SCHEDPHKGRFDDANCTSCHNQTDWSQVTNVRKNHPGLSLAAGHAKTKCEACHDQGNV
                     VAPSAGAECVACHAQVHDAKFGDRCENCHASIRWMGLPSKIGLRAHPLTGFPLEGLHR
                     DVDCRRCHSPKLSPQKRFRGLQFDQCADCHRDVHRGTLGAVDCATCHDVHGFWPTRFT
                     VEQHGRTEFPLEARHQFVPCSDCHESKRPRYLLTVKARACGDCHENPHGQQFAAEMRD
                     GGCAHCHSERGWESPKIDHSVWPLTGAHAESACDSCHTPSRQDRLSGRGATYRGVSRE
                     CAGCHREVHAGQFRLTEPERACDACHITASFGIEAFKHEELAGYPLEGGHAAVECSAC
                     HKKEKLRGGARVVRYRLGYQECADCHANPHARRKGQR"
     gene            complement(2764..3690)
                     /locus_tag="VFG22_06805"
     CDS             complement(2764..3690)
                     /locus_tag="VFG22_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415987.1"
                     /translation="MRFGWGPVVHRGGIGNVSRNSTKARRTRLAVLLVLLLLSVLSYA
                     LWGLGQDFYLSDLEARVEHSAYETLRPGGLIGHGYGIVGTILILANLLYLARRRLARW
                     SLGSMATWLNLHVFTGLLGSLLILFHSAFQFRTPIAVVTAASLGIVVVTGIAGRYLYA
                     LTPRPDRSALEQALESLDELTPGCSTRVYHALAIHKVTQVDTHRWLLAKLAAVPKWAS
                     EARARGFLVSYVIEDAPVSHVLDRKERRRFRKAKRAVRRYATREVVAVGASSLLQSWR
                     GFHRLLALILVLLVPVHIAVAWVYGYRWIFSE"
     gene            3750..>4189
                     /locus_tag="VFG22_06810"
     CDS             3750..>4189
                     /locus_tag="VFG22_06810"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET6415988.1"
                     /translation="MNVWIALGMFAAVTLALNLYWRKKEQVRTETGRRELDELRAMGD
                     LIPASIHPYIDPIRCIGSGACVYACPEKSVLTVVNGRGVLTNPLGCIGHGACASACPV
                     NAISLVFGTAKRGLELPQVDPDFQTNQPGVYIVGELGGMGLIRNA"
BASE COUNT          807 a         1327 c         1306 g          749 t
ORIGIN      
        1 gactgcgtgc gaatccgttg ctccgacgac gcgataccgg cgactgccaa agacgacgac
       61 ggatccgagc ccgaccccgg cggcttgacc gggcgcgaga tatgcgcggc ccccggcaat
      121 ctcgaccacc ttgaccgcca cggtgcggcg tgactccgcg cgcgctgaga ccgccaagaa
      181 gaagaccagc atgaatactg gagccgctct cagacgcctc atcgcgcggt gcctcccgga
      241 tgacagccaa ggcaggcgcc tccgctgctc gatacccggc gtccgtgctg ctgctgaagc
      301 ttcactgcat tgtgcgcgtg acaaccggtg caccgaacca ggcgaggtgt ctgagcagcg
      361 atgtgacagc tgttgcaggg cgctccgcgg tggggaccat gcgaaactgg gaagtgccta
      421 tcgtggttgg ccggcaccac catgccccca agccccgtca acgaagcggt cgatgagctt
      481 gcaatggcgc ttggatcgat gaacgccgga ctccatgcgc tcgccacgtg gcattgcgag
      541 cagtcctgcg gaaacggagc tgaagtagcc gtgccccgat gactcggatg aatgttggga
      601 tccagaaagt ccgacgcatg gcacgagaag cagtcgctct ggaccgtggt ccactgcctc
      661 tcgttgttgc gtagatggca ctccgtgcac gcggccagcg cgtgcatgcc cgtgagaggg
      721 aaacgcgttc gacggtggat ctcgaagggc tccatgtcgt cccaacgccg cgacgagtgg
      781 cagctcgcgc aacggccgct catgcgcgat tggtgcggat cactatggca gttctcgcat
      841 tcgcgagtga cgcgcttctc tccgctgtgg cactgattgc atgcagcaga ctcgtgccgt
      901 ccggtgagag ggaacccggt ccgagagtga tcgaagcctt gcccagcctt acccgtgctc
      961 atcttccaac cggcgggtgt gtggcagttg ccgcaatcga tcccttcggt gatcgagttg
     1021 tgattgggcg cggtggcagg ggcggccttg ggcttcggat ctttggccac ggcgatgccc
     1081 gcaagtccag caagagccgc aacgccgatg agaaagagaa gtccggacct catcgctgcc
     1141 cctttcgacg cgcatgggga ttggcgtggc agtccgcgca ctcctgatac ccgagtcggt
     1201 atctcacgac ccgcgcgcca ccccgaagct tctctttctt gtggcaagcc gagcactcga
     1261 ccgcggcgtg tcccccctcg agcgggtagc cagccagctc ctcgtgcttg aaggcttcga
     1321 tcccgaacga cgcagtgatg tgacaggcgt cacaagcgcg ctcgggctca gtcagacgga
     1381 actgcccagc gtgaacctcg cggtggcaac ctgcgcattc gcgcgacacg ccccgatagg
     1441 tcgctccgcg tccgctgaga cggtcttgcc gcgacggagt gtggcaacta tcacaggcag
     1501 actcagcgtg cgcaccggtg agcggccaca cgctgtggtc gatcttcgga gattcccagc
     1561 cacgttcaga gtggcaatgc gcgcaaccgc cgtcgcgcat ctcggcggca aactgttggc
     1621 catgtgggtt ctcgtggcag tcgccgcaag ctcgtgcctt cacggtcagg aggtacctag
     1681 gccgcttcga ctcgtggcaa tcgctgcagg gaacgaattg gtgccttgct tcgagcggaa
     1741 actcggttcg cccgtgctgc tcgacggtga agcgagtcgg ccagaacccg tgcacgtcgt
     1801 ggcacgtggc gcagtcgacg gcaccgagag ttccgcgatg cacgtctcga tgacagtcgg
     1861 cgcactgatc gaactggaga cctcgaaatc gcttctgcgg ggaaagcttg ggtgagtggc
     1921 agcgcctgca gtcgacgtca cgatgcaagc cctccagcgg gaaaccggtg agtggatggg
     1981 cgcgaagccc tatcttcgat ggcagaccca tccaacgaat gctcgcgtga cagttctcgc
     2041 atcgatcgcc gaacttcgcg tcgtgaactt gcgcatgaca agccacgcac tcggcaccgg
     2101 cactcggagc tacgacattg ccctggtcgt gacaggcctc gcacttggtc ttggcgtgac
     2161 ccgcggcgag gctcaagcca gggtggttct tcctgacgtt ggtgacctgg ctccagtcgg
     2221 tctggttgtg gcacgacgtg caattggcgt cgtcaaagcg gcccttgtgc ggatcctcgt
     2281 gacaagaagc gcattcggtt cggagtccga ggtatgtcgc gtttccgcgc cgattgcgga
     2341 ccttgtgaca gtcctgacat cgaaccgacc gatgggcgcc ctcgagcggc cagcccgatt
     2401 gaccatggtc gaaccgatcc ggtgtgccgc caggccagcg aaccagcgaa gcgtttctcc
     2461 caaggtgctc gacgtgacag ccaccgcagt cctgtcctcg gtactcccga ccgtggagac
     2521 ccgcttgtcg ccggattcga gatcccaggt cgtcgtggca gccgaggcaa gcgttcgcgt
     2581 cgattcgctt accggtggaa tggcactggt tgcaattcgc atccccatcg atctctgcat
     2641 gggccgtcgt gagcttgccc ggtgacagaa actgagcacc gcttgaacga ggtaccagcg
     2701 cgccagcaat cgccgccgct atgaccaggg ggcctacgat caccagcgct ttgatacgcc
     2761 cgctcattcc gagaagatcc agcgataacc gtaaacccac gctacagcga tgtggacagg
     2821 aacgaggaga acgagaatca acgccaacaa gcgatggaag cctctccacg attgcagcag
     2881 agaggacgct ccgacggcga cgacttcgcg cgtcgcatat ctgcgcaccg ctcgcttcgc
     2941 ttttcggaag cgtcgtcgtt ccttccgatc gagaacgtgg ctaacgggtg cgtcctcgat
     3001 cacgtagctc accaggaatc ccctagcccg tgcctcgctt gcccacttcg gcaccgcagc
     3061 gagcttggcg agtagccagc ggtgggtgtc gacctgagtg accttgtgga tcgcgagcgc
     3121 gtgatagacc cgggtgctgc acccaggcgt gagctcgtcc aaagattcga gcgcctgctc
     3181 gagtgcgctt cgatcgggcc gcggggtcag cgcgtacaag tatcggccag cgatgcccgt
     3241 caccaccacg atcccaaggc tggccgccgt aacgaccgcg atgggcgtcc ggaactggaa
     3301 cgccgaatgg aacaggatca agagcgaacc aaggagtccg gtgaacacgt gcaggttcaa
     3361 ccaagtggcc atcgatccaa gcgaccagcg ggcgagccta cgccgcgcca ggtagagcag
     3421 attcgccagg atcagaatcg tcccgacgat accgtacccg tgcccgatca gtcccccagg
     3481 acgcagggtc tcgtacgcgc tgtgctcgac cctggcctct aggtccgaga ggtagaaatc
     3541 ctgaccgagt ccccacaagg catacgagag cacgctcaag agcagtagga cgagcagaac
     3601 ggcaagcctc gttcgccgag cctttgtgct gtttctcgac acgttgccga tacccccccg
     3661 atgaacgacg ggtccccacc cgaagcgcat actagactac cccaagggta ccccagaaaa
     3721 acctcgtgtt cacacaaaag ggctaattaa tgaacgtatg gatcgcactg ggcatgtttg
     3781 ccgctgtgac cctcgctctc aacctctatt ggcgcaaaaa ggagcaagtt cgcacggaaa
     3841 cggggcgccg agagctcgac gagctgaggg ccatgggcga cctgattccg gccagcattc
     3901 atccatacat cgacccgatc cggtgcatcg gctccggggc gtgcgtgtat gcctgtcccg
     3961 agaagagcgt tctaacggtt gtcaatggca gaggggtgct gaccaatcca cttggctgta
     4021 tcggccacgg tgcctgcgcg tcggcatgcc cggtcaacgc gatcagcttg gtttttggaa
     4081 ccgccaagcg tggtctggag cttccgcaag tcgaccctga cttccagacc aaccagcccg
     4141 gcgtgtacat cgtgggcgag ctcggcggga tgggactgat tcgcaacgc
//
LOCUS       DASRZF010000150        28987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2167412, whole genome shotgun sequence.
ACCESSION   DASRZF010000150 DASRZF010000000
VERSION     DASRZF010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 28987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28987
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2167412"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1353
                     /locus_tag="VFG22_06815"
     CDS             <1..1353
                     /locus_tag="VFG22_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415989.1"
                     /translation="FYKMRYYQSALNVFDEISAMGPAHLFFGDTLEWLGLLASKLPES
                     SGIIEKVGRYGIGALDEFKEKNPKLYNQLLYMMGRHLYAQASFRQAIDVFQEVAPRSS
                     QYTYAKFFEGISFVRMRQARPAIASFRSILEALDAGDVKGVDQERMRNLAWISLARVY
                     YTAANQGKGGQIDGTLLGQSVESWTKVEQASEYWLDSLFESSWAFFLADEYSRALGNV
                     HTLYSPYFQDSFYPEALVLKAVTFFVNCQVDNAEATVAQFHDRYDPVKQELDAVLQKH
                     QDNADFFEFLQQVRANEDDLSPRVRPIVSTALSDRTVLQHLEYVSILDAEEARLGKSS
                     AEFQGAPVGAKILEDVALARSFAVDQAGDLARGRYQRLIRELRDLSNQVDTVELEIAT
                     FRRGQIDQELQQQMSLAKQSRGGDVNVDEEHQLWPFDGEWWRDELGFYRQQVTNLCTR
                     "
     gene            1359..4958
                     /locus_tag="VFG22_06820"
     CDS             1359..4958
                     /locus_tag="VFG22_06820"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6415990.1"
                     /translation="MRYTASFLFGLLALVVGGHAAAEQVPMSTAPMCIPESTERAIVA
                     CPDGARKEAAKEGGNAPVSRMKAAAPKRSGTQGPTGPSLEIDMSTRLGREQTRARAEG
                     LLEKEIAILQRLVKNSAEDDPRRPDVLLRLAETQFEMQIAKNAKVRSFDDPVYEACTR
                     DEDKARCKEALDGQKAAQAELNAIREDSIRTYATLVRDHPNFKRMDEVLFSLAFALQE
                     VDQFDKARSVYRRLIKDYPKSRFVPNAYLSFAEYYFNESDMEAAGRFYRKVIEFPPKE
                     NSVYGYALYKIAWVEYNLERYRQSLQGFVDILEFARKNKYANDANNLARQARKELVLP
                     YSHYGSPGKALDFFRRYSKDDAEAHEMLEHLAELYYDTGQWAEAITVYHKLMADGPRS
                     NSLCDWQTKVTNAVISSRPNQEQIVELKRLVDVYRTFNQSGKPADKVEECKVETATTM
                     LWLGTSWHREAVGTDAAPGTKNKKTMSQAAVLYRTLLKEFPDMEQMEFKNIDKRDWPT
                     EYKISYFYAELLWKMDEWGQCGPAFDHVVELNPQGEFTSDAAYAAVLCYNNLYQQQYV
                     PRETETNFVSKSDQRALKNTKAAPKNEFAPKKLTPLQEGMLEAFNRYVCYVDKAEDLA
                     TIKYRRARIYYEANRFEEAAVLFRDIAFNHKDSELSEYAANLYLDCLNVLGTQIDEPR
                     VACVQELSASIDPMSASFCGTSQLADEHPDLCGTLGTLQCQVRRKEAETYQKTKQFKK
                     AAATYVRIFRRHEECGEMDEMLYNAAINFEAAHLLGRAIQVRTVLTERYPESELSKKA
                     IYLIGQNFQALAYYEQAAKYYEQFARKFPGEDGSRCTAEDKENGTCPNAIEGLEQATF
                     FRIGLGDDKAAMEDADLFARNYQRKLPRQTSQVMFSIGSIYERQKRWFEVISHYRDYL
                     KNYSKVGMPHQVIEANTAIGRAFWALNKKGEAKPYFQAAVKGWSSGAPKRILSLSKTP
                     KESKVLYLREALDSAAEAQFHMSEYEFEQFQKVAFPRYAGGKSLASVKKWSDSQFKKW
                     VQRKQSVLRTAEAEYAQVAKMTVEVGGVEMKSAPWQIAAAARTGEMYRSFVDEFRDAP
                     VPREIEKDANLYDIYVGALDEVSEPLQRQAIDKFEFCLKTATQVRWFNNWSRSCEQEL
                     NGLNPRKYPVAAELRGEPNYVKATVGDPGAVDLGAINEQRLNTKDAVANREEP"
     gene            4958..6235
                     /locus_tag="VFG22_06825"
     CDS             4958..6235
                     /locus_tag="VFG22_06825"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6415991.1"
                     /translation="MRNHQVAFIAIALMAGCGGGGSSSGSSTTPPADVARTAGGEAIT
                     TADGSAVSKKAHGKWESALEEFEANEKAGWTSAKCNASESSFERTAEAQGGGFAEAHY
                     MAGLSLSRCGDSAAMKHYERALGADPKFCKARVAIGLQDLHDGRTSQAKSAFEQSIKD
                     DPQCTSGYTNLAIVQRAQGESEEALKNLRRALAIESDYLSAFNQMALLHYDRGRAGNA
                     AELDLAEVVCRQAQMLDADYAPIYNTWGLIKVYKGDVISALRFFQRAITLDPDLFEAQ
                     MNFGEVTISFRGYEDGRAAFARAVELDPKNYDAVIGLGTALRGLNQFAEAETQYERAK
                     QLDDRRPEAYFNLGVLYQDYMSGSVADLKKAKIYFTEFLKRAGKKTEFRASVEDVSNR
                     CAEKSSKSRGAPKCRPGRMQNIDISVEAMQVTG"
     gene            6245..6514
                     /locus_tag="VFG22_06830"
     CDS             6245..6514
                     /locus_tag="VFG22_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6415992.1"
                     /translation="MKQKTSKFIVQTFVFIALFSQPAFLVPSAEAQSAPAPETTYDFE
                     DDLVTGDLVRPDGEQLIVRRRGRRASLIQIREHFIPEMLKSVEDL"
     gene            6527..8554
                     /locus_tag="VFG22_06835"
     CDS             6527..8554
                     /locus_tag="VFG22_06835"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HET6415993.1"
                     /translation="MTESSHNTSVAFSVYDGDALLSSETLSQEIIKIGRLQTSDIQLD
                     DDKVSRMHAVIEVTAPGEVFIIDLGSASGTLVNGEKINKRKLQTGDQVQVGNKRMHID
                     IITGTVAVASSPPVAMAAGGGRATPVVDERAMRAMLDEESEPRVKATVTASKADEESG
                     PKFNPFEVGAKRLSRTPFGNSREEDGPEYGLVATTAALPAHEIETDERAIEVAVLWGD
                     RNVLNVDYLTPPRDYYVGEPNGEDVDYVLGHDAIGVDRMPLCIAQGAGVTAIVPDGAK
                     GEATIGDQTRSFDLMRGQGDFRPSSTVAGAMEFPVPEGGSVRMKHRDFTFIIKDVKAG
                     KKVAGHAKMDIRPMIYVAGAALFASLVLTMFYFQPPRPRGLSSDLLATDSRLIQFLME
                     PEELEEPEPEWLNGQDSSGGGTGKAHEGEQGQMGDEKAEKTKNKYGIQGPQDNPDPVM
                     AREQAEEEARTAGIIGTLASMQGSFNSPTSPYGADFALGNDPSSALGAMTGNDIGQNF
                     GFGGLGLRGTGRGGGGLGQGTIGMGNFGTIGRGGGRGDGEGGYGRGVGGFRGRSAGVP
                     QVRSGGAEVRGSLSKEVIRRVVQRHINEVKFCYEQQLNARPDLQGRVTTKFVISPTGS
                     VQSAMVGDSTLRNEAVESCIVRAVRRWTFPAPDGGGVVVVNYPFLLDAAGQ"
     gene            8762..9631
                     /locus_tag="VFG22_06840"
     CDS             8762..9631
                     /locus_tag="VFG22_06840"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6415994.1"
                     /translation="MRAHSFGVVLVGLILMGGNCSRARVESMNEMNAGVELAEMKRFN
                     EAAQSLQKAAVLDPTNHQAYFNLAIVEIELRSFSAAREALERAIVAKPDVAGYHEKLG
                     TVLMELEDWKGAQVAFTEALELDPSLFKAHFKLGRCSEELEDAQNALYQYTDAIKTGP
                     RFLPSYSALGRLYADLRYPDQAAQVLREALKVAMPATEEEANIHHLLGTVYQQQRKYD
                     EAIAEFQAALGIVPGMSDALFSLGWTYALQGNDEEAKRYLDKYVMMAGTDAPEHYVKA
                     ARDRLAELRTESP"
     gene            9647..19699
                     /locus_tag="VFG22_06845"
     CDS             9647..19699
                     /locus_tag="VFG22_06845"
                     /inference="COORDINATES: protein motif:HMM:NF025912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6415995.1"
                     /translation="MSEEQSGDEVRELTAGEVAELEKLDDAIAKFEQQKRWSDMIRSI
                     LKKAALIQLPAEKVELFRQAGTLYLERSSNQAEAIKCFEELLEYEAHDVEAIQRLKEM
                     YEKRRDWEKLIRTMQREVELLPEADQLIAYAEMADLATQRLRKPEVCIELWQKVLDAD
                     PENPSALENLAQLYERAREWEPLAEVLETLVAADSDVTSMKQQLQKLGMIYADKIGND
                     EGAIRAFTKLLELDPSDRRAQEQLKRRYVSLGAWDELEAFYAANEQWDELIRIVERQA
                     EAQETAPEERIPLLFRAARLWEEMKGKPDRAARVYEKILGADPDNVQAAEALTPIYEE
                     AHDARKLVSVLEVRFAHASDDDAKVELLLKIGQVFEERLRQPEVAFERYLEAFALAPT
                     HDEARIDVERLAEEMNGWDRLVSGFESAIDAVSDPHQAVPIRLSYGQTLAQLDRIDDA
                     IEQYQLVYELESNNLAAITALGELYVRTERFRDLLDIYERRIELEEDPEARLEIAYRR
                     AKLFAQALNAPAPAIEAFEGIIDEYGVEQGDACRELERLYEAESRWQDLAQLLERRVE
                     ARPESHEELAALKFRLARVCESQLDDKLRAVELYREVLSILAEHDGARARLEALLDDD
                     EVAAEASRVLEPVYEMSDDWNHYINALRVQHRFSTLPREKLQLLTRVADTYFQHIGDA
                     QRGFVSLSEAFREMPSDLGTLQRLEMLATEQGLFEAVADLTGDLAANVSDVSLSRSLW
                     IKTAEIHESRLDDTEAAVSAYRKVLETDPGDFEVLGALDVLYRGAERWHEVLGVLRAR
                     AEAYADPDEKIGVLAEMARIHADLLDEQEPAIALQNEILELDPSNRGALGTLDDLLAR
                     QGLWSDLADNVERQLSMTDDPQEQTALLLRLAEIREQRMGSIDSAIEIYRDVLTNDAS
                     NPAALGALERLLESAEYQAAVADILEPIYKNGGASARLVDVYEIQAHHATAPERRLGL
                     FQEIAKLQEFSLSDLRSAFESYARAFREDPGHPKAKEELERLSAAVGASDALASVYEE
                     VVTSLDDPQLSTALLLRAAEIREDDLGQLDRAVEHYQNVLEIDEEHRYSADALERLFQ
                     QTERYEDLASIYRRKARMLPSPSDQRDQLVKAAAIYEDILEKPAEAIAVHQQVLEIDP
                     TDIESLDRLIELELGLGKWSALLQTYERKAEVVSDPDARKEIFAAMGRVHEAQLDDGD
                     KAIDAYQRVLEIDPDDVQALRHLDALHESMSQWRDLLQVLERQAELATDEDLRIELCF
                     RIGELWRRHLEDPKQAIEIYRDILAEAPEHQRTLDALTAMTRDAVEPLAAAEALEQVY
                     LAAGEYRKLAEVREVQVRFTEEPAECVEMLQQLAEIYEIQLEDPPSAFAAFVRALPLD
                     TRNELTLSSLERLAEQTGNWHEAASRYDAEVQRMREQSPADVVHLAKRSAVINEVQLD
                     DVDAAISRYQFVADVDPSDVTAIEALDRLYEQTERWDDLAATLRMEAAIASTPEAVLN
                     LQFRTGQVFQNQLGKMDSAIDQYREILAAAPEYAPAVSALEDLFSSGVQPMAIGEVLE
                     PLYRMQGNWDRLLNVQEVQVEHQKDPIERVVMMHRLAEIAEDKTSDHGRAFEWMQKAL
                     LEDPSHDHSLGEAERLSAIVDGWTELATTYANGIQHAGDPLNKAELGRRLAKVYENEL
                     HDLERAEETYRFVLGVEAHDDKALKALDRIYTEHGAYRALCEVLRKRIEGCNNSSELI
                     DLHYRLGTVLEQRLGCTEDAIEVFTKLLDEYDSEHSQTIHALEGIYANLGRWPELFAA
                     FEKELSIALGDSQQADVFARMAKLSTNRLSDPEKAIGLWQRVLDLRGEDPEALNALGD
                     IYASQGNWRDLVDVLEREAGIADDDAFRIQVYSDLARVWYEKLERERNAIENWERVLD
                     IDPGNAYALFSIAEIQRAGGQSRERADTLHRVIDVGAATLEDADLITVYMQLGHIYAN
                     ELNQPGDAVEAYRNVLTIDPRFFEALDSLELIHRKEGDWEEAIGVMEQRAQALADPQE
                     KIRQLIAVTELWETELGNKEGAVGAFGRILEVDAMHEYAFSESQSLLRNAERWDELID
                     LYVTRIEATESRKERIELLRAAARVYEEHLDDGEQAYHTLYIAWTEDFANKNTAVDLE
                     RVTAANDKWNELLTSANQVLQDGQEPETQIAICLCCAKWYGQDLGHPEYAIPYYEQIR
                     ALDPSNAKAWWQLGDLYETTHQWDKLAQALAKLVELTDDDDVKANTYVRMGDLADDRF
                     AMRQEAEDYYRRALQLQDKHVGALEALERIYREDGRSNKLLNILERKAEAVEDVESVV
                     SALLQVAEAYEERFDDPKAATENYERAAELSPTDLTALRGLERLYTQDGRYQDLIGIL
                     EREYEVVATERERIEILMRLAKMWEEEFRKPENAAERLEQVLDIDPLHDEALCGLEEL
                     YRTMQSWDDLVRTYDRHASATADTEERVRIFRELGQVYATQKEDVEQAIDSYLNALNL
                     NEQDLFSLQALTKLYERKGDYAAALDMMGQLVGVSDDPSDQVALKHRMGRILDQELGD
                     RVSALEHFQTAIDLDPAHLPSLEAMRSIYVDSGDWAAAAKTLCLEVEHTEKDRIRAVR
                     LVELGRIRAERLDDEDGAVEAFEAAIEADADNEDAMLPLVDAYVSAGRHENAFPLLRT
                     LVQRSDKREHEEQHRLASMLGETATMLGHADEAVSAYERAYELDSNDSVTLKGLAGAH
                     FRAERWDDAFKYHQLLLVQHRDALGRDEITEIFYRLGVVKREQGEEKKARNMFDKALE
                     EDTHFVPALEAMVALQTADGDWSSVVEYKKRILDVADEPSVRAAILVELGDLWHEKLG
                     EPGSAAEAYTESLDLEPENHVVLHKLLVAYQAAERWSEAIDVIERVRGIEERDEAKAK
                     YTYTIGVILRDKLEDEDAALERFNDALDIDCTQLKPFEAVNKLLNERKDWKALERAYR
                     KMLHRIINQGNTDLEHNLWHTLGIIYRDRQRNFDAAAESFQMAAGLKPDDPVGHQILA
                     ELYATMPEKTEQAIVEHQWLLGADPNRLDSYRALYKLYFDARAYDKAWCIARTLSYFE
                     KADEEQRKFYQQYKQDREIQPKTRLSNEAWVKELMHADQDLYISKIMEVIAPAVRASM
                     AMTDKKLNLNKRKPEDLANPSLALARQFKLAQDVLNVTIPVRLFIQKELSGALRDEPR
                     SNPPAIITGYTLLSGYRPVDLAFVCGRHLTYYRGEHFIRTMYNSHTDLRTLLLAAMRV
                     VGMGGGDASVESTAKQLAKYMDQSHLDVLKQVVRKFVDAGGQADIKRWMQAVEVTALR
                     AGLLLCDDVDTAVRMTQQIPSESTADLAPRDKVKEIVLYSVSESYFRLREQLGIQIKV
                     "
     gene            19739..20242
                     /gene="tsaE"
                     /locus_tag="VFG22_06850"
     CDS             19739..20242
                     /gene="tsaE"
                     /locus_tag="VFG22_06850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HET6415996.1"
                     /translation="MGMLELELHSEADTVALARTLAAVLAPGDVIGLEGGLGAGKTTF
                     TRAAVHGLGVPAETAVTSPTFALLHQYQGRLPIAHADFYRLGDEAELEELGVDELLEE
                     GGVLFVEWGKKFPAMGARTALWVELEILSDLARRARFRPESARGDAIISALSKRGSDE
                     DLPSCGP"
     gene            20288..23647
                     /gene="carB"
                     /locus_tag="VFG22_06855"
     CDS             20288..23647
                     /gene="carB"
                     /locus_tag="VFG22_06855"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009455.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HET6415997.1"
                     /translation="MPRRDDIESILLIGSGPIVIGQACEFDYSGTQGAKALKEEGYRI
                     ILVNSNPATIMTDPEFADRTYVEPLTPEILETIIEREKPDAVLPTLGGQTALNLALSL
                     ADRGVLEKHGVELIGANVEAIRKAEERELFKAAMDKIGLESARAEVARTVDHAHEIAE
                     RIGFPIILRPSLTMGGSGGGVAYNKAEFDTAIRWAFQCSPVHECLVEESLLGWKEYEL
                     EVIRDKADNFGVICTIENFDPMGVHTGDSITVAPAMTLTDREYQRLRDAARAIITEIG
                     VETGGSNIQFSVNPADGRVIVIEMNPRVSRSSALASKATGYPIAKIAAKLAVGYHLDE
                     LRNDITGTSAAFEPTIDYVVTKIPRFAFEKFAGADPRLTTQMKSVGEAMAIGRTFKES
                     LQKAARSLETGRDGLTSLLKKVDYRDLAAQVRRLIETGEAINTAKPMLADLPDATDAE
                     LRESILDLVRTPLADRLWYLADGLRVGLTVEDLHGATQIDVWFLEQMRQILAFEGTLK
                     ESTGKALGEGLLRDAKALGFSDAQIATLRGETSKSVRAERAAKGVEPVFSRVDTCAAE
                     FPALTPYMYSTWGLDSEARVGGDKKKVMILGGGPNRIGQGIEFDYCCVHASFALKEMG
                     FETIMVNCNPETVSTDYDTSDRLYFEPLTFENVMHIVEVEKPDGIIVQFGGQTPLKLA
                     VPLSREGVPILGTTADAIDRAEDRERFEELLTKLGLDRPRGGIAKGAKDAFAVAERIG
                     FPVVVRPSYVLGGRAMEIVHSQEELARYMTVALEAVADAEAQTILVDEFLKDAIEVDV
                     DCVSDGHDVVIGGLLQHIEEAGIHSGDSSMSLPAHALPDDVLATLRQSTRALALELGV
                     VGLMNVQFAVRGSAVFVIEVNPRASRTIPFISKAIGVPLAKIAAKVMAGSTLRELGFT
                     REIVTEHIAVKESVFPFTKFPGVDTILSPEMRSTGEVMGIADNFPEAFYKALLSAYTE
                     LPDAGKVFISVKESDKPAAAQLAHRLKGLGFEIVATSGTRNVIAEAGVDAENVHKVGE
                     GRPHIVDIIQNGEINLVINTTAGAKAIRDSRSMRRQTVLSDVPYFTTIAAAVAAVDAI
                     EARRRRPLSVCSLQEYHARNRELATLLPPSD"
     gene            23690..24160
                     /gene="greA"
                     /locus_tag="VFG22_06860"
     CDS             23690..24160
                     /gene="greA"
                     /locus_tag="VFG22_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HET6415998.1"
                     /translation="MERVPMTPAGHAALTAELNALKAERPKISQEIGVARDHGDLKEN
                     AEYHAAKEKQGMCEARIAEIEDKLSRAEVIDPSTLSGEKVKFGAIVELEDMDNGKMVT
                     YRLVGPDESDIKEGKISITSPVARALVGREVGDEVRVETPGGLRHYEVSDVRWG"
     gene            24192..26288
                     /locus_tag="VFG22_06865"
     CDS             24192..26288
                     /locus_tag="VFG22_06865"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HET6415999.1"
                     /translation="MPTLLDHPVETLPGIGSTTGAHLRARGFESVGDLLWLLPRGYDD
                     QRAPTPIHELRCGEYAVVEGLVRRARSFPRGGRLGFEVRLDAPEAPAEVDGYRELKLV
                     WFRAIPGLARRFAEGERVRVAGKVTDYRGTATMAHPESLPADVRGAIEPRYPEVPGVR
                     RKILRRAVRAAVDRAVAEVPDLIPAALRAEVGACTVAEALRSIHVPDPEAFDAVANAE
                     MLGPAHHRLALEELVLWELALRLRRASERGGLSRAFEIGASVSAASGAFPFELTAAQR
                     AAVRDVSAALCSETPMRRLLQGDVGCGKTAVAMVACAQVAAGGAQAVFLAPTELLADQ
                     HAETLRPTAEKLGLRLGVLTGALNKDHRRSMLDRLATGAVDLVVGTHALLSGDVRFAE
                     LGLVIVDEQHRFGVAQRLRLGSRGSTRTPHLLVMTATPIPRSLALVLYAGLELTTIDA
                     KPPGRVPVTTKMLSRLKRDAVYRQIERALEAGGRAFVVCPAISTSEELVGVDQVFTEM
                     EARFGSARVGRIHGKLSGDERRVLMRQFSEGELDVLVGTTVLEVGVDVPKANIMVIEQ
                     AERFGLAQLHQLRGRVGRAGQRSACLLMFSEPLSADAEARLQALCETDDGFRLAERDL
                     ELRGAGHLFGYRQSGASGLQFANLARDQSLLQRSGDLADRIIGEDPGLSKPEHAAARA
                     AIERWESCGAVREEAG"
     gene            26351..27079
                     /locus_tag="VFG22_06870"
     CDS             26351..27079
                     /locus_tag="VFG22_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HET6416000.1"
                     /translation="MPLVHHAQREVTLKLVYYGVGLGGKTTNLETIHAQTRPEARGKL
                     VSVNTEAERTLFLDFLPLQLGTFRGYTVRLHLISVPGQIAQDSTRQMVLRHVDGVVLV
                     VDSQADAIEGNNYSIRNLDYNLRQQGVDPDRIPMVVQYNKRDLPGILDVDELAERLGV
                     PEGIPELVASARDGWGVFETVKSIVRICMHQLGDPSLRGDGRVECLLGEPRDRFYPSG
                     PVSMIHALRPEEELELEDLELISG"
     gene            27084..27959
                     /locus_tag="VFG22_06875"
     CDS             27084..27959
                     /locus_tag="VFG22_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6416001.1"
                     /translation="MNPPGQVSGGHDVRIIAPTIEMLPVRTPTLPPATHTNSFLVGTG
                     EAVLIEPATPYREEQDRMADWVRSARLKGVEPIAILATHHHPDHVGGAMALRERLELP
                     LWAHAMTAQRLDGIVEVDRLIQDGERIELDGPTPTSLSALHTPGHAPGHLCFTDEVSN
                     IMIAGDMVASVGTIIVEPTDGDMLLYLESLEKMSALEPAGLLPAHGDLITEPEAILRF
                     YIQHRLMRERKVLEALEERGKPSRPRHLVASAYADTAKALWPLALQSIEAHLIKLERE
                     GRIAKEGDRWAPITH"
     gene            28291..28623
                     /locus_tag="VFG22_06880"
     CDS             28291..28623
                     /locus_tag="VFG22_06880"
                     /inference="COORDINATES: protein motif:HMM:NF017451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HET6416002.1"
                     /translation="MGKKNVYERARAVVRELAPALQTIRDCDKSLADQLKRAAQSLVL
                     NIAEGRGSDPGNARARFSTACGSAKEVRAALNVASDWGYIESTTAAHLDEKLDEVCAI
                     TWCLGRRL"
BASE COUNT         5957 a         8702 c         9247 g         5081 t
ORIGIN      
        1 ttctacaaga tgcgctacta ccagtctgcg ctgaatgtgt tcgacgagat cagcgcgatg
       61 gggccggcgc acctcttttt cggagacacc ttggagtggc tcggactgct ggcgtccaag
      121 cttcccgagt cctcggggat catcgagaag gtggggcgat acgggatcgg cgctctcgac
      181 gagttcaaag agaaaaaccc gaagctctac aatcagttgc tctacatgat ggggcggcac
      241 ctctatgcgc aagcgtcgtt tcgtcaggcg atcgatgtct ttcaagaagt cgcgccacgc
      301 tcgtcccagt acacgtatgc gaagttcttc gaaggcatct ccttcgtgcg catgcgacaa
      361 gcgcgcccag cgatcgcttc gttccgttcg attctcgagg cgctcgatgc cggggacgtc
      421 aaaggggtgg accaagaacg catgcgcaac ctagcgtgga tatcgctcgc tcgcgtctat
      481 tacaccgcgg ccaatcaggg gaagggcggc caaattgacg gaacgctcct cggtcagtcg
      541 gtcgagtcct ggacgaaggt cgagcaggcc agtgaatact ggctcgattc gctcttcgag
      601 tcgtcctggg cgttctttct ggcggacgag tactcgcgcg cgctcggcaa cgtgcacact
      661 ttgtactcgc cctatttcca ggactcgttc tatcccgaag cgctcgtgtt gaaggcggtg
      721 accttcttcg tgaactgcca agtcgacaat gcagaagcca cggttgcgca gttccacgat
      781 cgctacgatc cggtcaagca ggagctcgac gccgtcctgc agaagcatca ggacaatgcc
      841 gatttcttcg agttcttgca gcaggttcgc gccaacgagg atgatctgtc gccgcgggtc
      901 cgtccgatcg tatcgaccgc tctcagcgat cgaaccgtgc ttcagcatct ggagtatgta
      961 tcgatattgg acgccgaaga ggcacgcctc ggtaagtcga gcgcggagtt ccaaggcgct
     1021 cccgtcggcg caaagattct cgaggacgtg gcactcgcga gatcattcgc agtcgaccag
     1081 gccggggacc tcgcgcgcgg ccgctaccag cgactgatcc gcgagttgcg ggacctcagc
     1141 aatcaggtcg atacggtcga gctcgagatc gcgacctttc gtcgcggtca aatcgatcaa
     1201 gagcttcagc aacagatgtc gctcgcgaag cagtcgcgag gcggcgacgt gaacgtggac
     1261 gaagaacatc agctctggcc gttcgatggg gagtggtggc gagacgagct cggattctat
     1321 cgacaacagg tgacgaacct atgcacgcgc taacgaaaat gaggtacacg gcgagctttc
     1381 tctttggatt gctcgcactg gtagtcgggg ggcatgccgc ggcagagcaa gtgccgatga
     1441 gcaccgctcc gatgtgtatt ccggaaagca ccgagcgagc gatcgtggcc tgtcccgacg
     1501 gggcacggaa ggaagcggca aaggagggcg gcaacgcgcc tgtgtctcgc atgaaggcgg
     1561 cagcgcccaa acggagcggc actcaaggtc ccacgggccc ttcgctggaa atcgacatga
     1621 gcacgaggct tggtcgggag cagacacgag ccagggctga gggtcttctg gaaaaggaga
     1681 tcgccattct tcaacgtctg gtcaagaaca gtgctgaaga cgatccgcgt cgcccggacg
     1741 tgcttctccg tctcgccgaa actcagttcg agatgcagat cgcgaagaac gccaaggttc
     1801 gttccttcga tgacccggtc tacgaggcct gcactcgtga tgaagacaag gcgcgctgca
     1861 aagaggcgct ggatggccag aaagccgctc aggccgagct gaatgcaatc cgggaggaca
     1921 gcattcgcac ctatgcaacg ctggtgcgcg accatccaaa cttcaagcgc atggacgagg
     1981 ttctcttctc gctcgccttt gcgctgcagg aagtcgatca gttcgacaag gcgcgctcgg
     2041 tctaccgacg cctcatcaaa gactacccga agagtcgttt cgtaccgaac gcctatcttt
     2101 cgttcgcgga gtactacttc aacgagagcg acatggaggc ggccggcaga ttctaccgca
     2161 aggtcatcga gttccccccg aaggagaact cggtctatgg ctatgcgctc tataagattg
     2221 cctgggttga atacaacctc gagcgatacc gacaatcctt gcagggcttc gtcgatattc
     2281 tcgagttcgc tcgcaagaac aagtacgcca acgacgcgaa caatcttgcg cgccaggctc
     2341 gcaaagagct cgtgttgccg tactcgcatt acggctcgcc gggcaaggcg ctcgatttct
     2401 tccggcgtta ttccaaggac gatgccgagg cgcacgaaat gctcgagcac ctcgccgagc
     2461 tctactacga caccggccaa tgggccgagg cgatcacggt ctaccacaag ctcatggccg
     2521 acgggccgcg cagcaattca ctttgcgatt ggcagaccaa ggtcaccaac gcggtgatct
     2581 catcgcggcc gaatcaggag caaatcgtcg aattgaagcg cctggtcgac gtctacagga
     2641 cgttcaatca gagcggaaag cccgcggaca aagtcgagga gtgcaaggtc gagaccgcca
     2701 caacgatgct ttggctcggg acgtcttggc atcgcgaggc cgtcggcacc gacgccgcgc
     2761 cgggaaccaa gaacaagaag acgatgtcgc aggcagccgt gctgtaccgg actctcttga
     2821 aagagttccc cgacatggag cagatggagt tcaagaacat cgacaagcgc gactggccga
     2881 cggagtacaa gatttcgtac ttctacgccg agctgctctg gaagatggat gaatggggtc
     2941 agtgcggccc tgcgttcgat cacgtcgtgg agttgaatcc tcagggtgag ttcacgagcg
     3001 acgcggccta cgctgcagtc ctttgttaca acaatctcta ccagcagcag tatgtcccgc
     3061 gcgagacgga gacgaacttc gtcagtaaga gcgatcaacg ggcgctgaag aacaccaagg
     3121 cagcgccaaa gaacgagttc gcccccaaga agctgactcc actccaggag ggcatgctcg
     3181 aggcattcaa tcgctacgtt tgctacgtgg acaaggcgga ggacctggcg acgatcaagt
     3241 atcgacgtgc tcggatctac tatgaggcga atcgcttcga ggaggccgcc gtccttttcc
     3301 gagacatcgc cttcaaccac aaggacagcg agctctccga gtatgcagcg aatctctatc
     3361 tcgactgcct caacgtgctt ggcacgcaaa tcgacgagcc gcgggtcgcc tgcgtgcagg
     3421 agctgagcgc ttcgatcgat ccaatgtcgg cgtcgttctg tggcacctcg cagttggcag
     3481 acgagcaccc agatctttgc ggcacgctcg ggacgcttca gtgtcaggtt cgccgaaaag
     3541 aagccgaaac gtaccagaag accaagcagt tcaagaaggc cgcagcgacc tacgttcgga
     3601 tcttccgccg ccacgaggaa tgcggcgaga tggacgagat gctctacaac gcagcgatca
     3661 acttcgaggc ggctcacttg ctcggtcgcg caattcaggt gcgcaccgtg ctcaccgagc
     3721 gttatccgga gagcgagctt tcaaaaaagg caatctactt gatcggtcag aacttccagg
     3781 cactcgcgta ctacgagcag gccgcgaagt actacgagca gttcgctcgc aagttcccgg
     3841 gtgaagacgg gagccgatgt accgctgaag acaaagagaa cggcacgtgt ccaaacgcca
     3901 tcgaaggctt ggagcaggcg acgtttttcc gtatcggact tggtgacgac aaggccgcca
     3961 tggaggatgc cgacctcttc gcccggaact accagcgtaa gcttcctcgc cagacgtcgc
     4021 aggtcatgtt ctccatcggt tccatctacg aacggcagaa gcgatggttc gaggtgattt
     4081 ctcactaccg cgactacctc aagaactaca gcaaggtcgg catgccgcat caggtgatcg
     4141 aggccaacac cgcgatcgga cgggcattct gggctctcaa caagaagggc gaggccaagc
     4201 cgtacttcca ggcggccgtg aagggatggt cctcgggcgc accgaagcga atcctctccc
     4261 tctcgaagac acccaaggaa agcaaggttc tctaccttcg cgaggcgctc gacagtgccg
     4321 ccgaagcgca attccacatg tccgagtacg agttcgaaca gttccagaag gtcgcttttc
     4381 cgcggtacgc cggaggcaag agcttggcca gcgtgaagaa gtggtcggac tcgcagttca
     4441 agaagtgggt gcagcgaaag cagtccgtgc ttcgcacggc agaggctgag tacgcgcagg
     4501 tcgccaaaat gaccgtcgag gtcggcggtg tcgagatgaa gtcggcgcct tggcagatcg
     4561 ccgctgcggc tagaacaggc gagatgtacc gaagcttcgt ggatgagttc cgcgacgcgc
     4621 ctgttccgcg tgaaatcgaa aaggacgcca atctgtacga catatacgtt ggggctctgg
     4681 acgaagtgtc cgaaccattg cagcggcagg cgatcgacaa gttcgagttc tgcctgaaga
     4741 ccgcaacaca ggtccgatgg ttcaacaatt ggtcgcgttc ctgcgaacag gagctcaatg
     4801 ggctcaaccc gcgaaagtat ccggtcgcgg ccgagcttcg gggtgagccg aactacgtga
     4861 aggccacggt tggcgatccg ggcgcagtgg acctcggtgc gatcaatgag cagcggctga
     4921 acacgaagga cgcggtcgct aacagggagg agccgtgatg cgaaatcacc aggtagcatt
     4981 cattgccatc gcgctcatgg caggctgcgg tggaggcggt agtagctcgg gctcgagtac
     5041 cacgccgcct gcagacgtgg cgcggacggc cggaggcgag gccatcacca ctgccgacgg
     5101 aagcgctgtc agcaagaagg cgcacgggaa gtgggagtcg gcgctcgagg agttcgaagc
     5161 gaacgaaaag gctggctgga cctcagcgaa gtgcaacgcc tcggagagct cgttcgagcg
     5221 aaccgccgag gcgcaaggcg gtggtttcgc cgaggcgcac tacatggcgg gtctctcgct
     5281 ttcacgctgt ggtgactcag ccgcgatgaa gcactacgag cgagcgctcg gcgctgatcc
     5341 gaagttctgc aaagcgcggg ttgccattgg gctccaagat ctccacgacg ggcgcaccag
     5401 tcaggccaaa tcggcgttcg agcaatcgat caaggacgac ccacagtgca cttccgggta
     5461 cacgaacctc gcgatcgtgc agcgtgcgca gggtgagagc gaagaggctc tgaagaactt
     5521 gcgccgagca ctggccatcg agtccgacta tctttcggcc ttcaatcaga tggcgctgct
     5581 tcactacgac cgcggacgcg cggggaatgc ggcagagctg gacctcgcgg aggtcgtatg
     5641 ccgccaggct cagatgctcg atgccgacta tgcgcccatc tacaacacct ggggcctgat
     5701 caaggtgtac aaaggcgacg tcatctccgc gctgaggttc ttccagaggg ctatcacgct
     5761 cgatcccgat cttttcgaag cgcaaatgaa cttcggcgag gtcacgatct cgtttcgagg
     5821 ctacgaggat ggacgcgcgg ccttcgcacg cgccgtcgag ctcgatccga agaactacga
     5881 cgcggtcatc gggttgggga cggcgcttcg cgggctcaac cagttcgcgg aggctgagac
     5941 gcagtacgag cgagccaagc aactcgatga tcggcgcccc gaggcctatt tcaatctcgg
     6001 ggtgctctat caggactaca tgtcgggctc ggtggcagat ctcaagaagg ccaagatcta
     6061 cttcaccgag ttcttgaagc gcgcaggcaa gaagacggaa ttccgcgcga gcgtggaaga
     6121 cgtcagcaat cgttgcgccg agaagtcgag caagagccgc ggcgcgccga agtgcaggcc
     6181 agggcgaatg cagaacatcg acatctccgt cgaagccatg caggtcacgg gataagggcg
     6241 aatcatgaaa cagaagactt cgaagttcat cgtacaaaca ttcgttttta ttgcgctatt
     6301 ttctcagccg gcattcctgg tgcccagcgc ggaggcgcag agcgcgcccg ctcctgaaac
     6361 gacctacgac ttcgaggatg acctcgtcac gggtgacttg gtgcgtcccg atggcgagca
     6421 gctgatcgtg cgccggcgcg gacgccgagc gtcgctgatt cagattcgag agcattttat
     6481 tcccgagatg ttgaaatctg tggaagattt gtagtggggg atgcgggtga ctgagtcgag
     6541 ccacaatact tcggtcgcgt tttccgtcta cgacggcgac gcgttgctca gcagtgaaac
     6601 gctgagccaa gagatcatca agatcgggcg tctgcagacg agcgacattc agctggatga
     6661 cgacaaggtc agccgcatgc atgccgtcat cgaggtgact gcgccggggg aggtgttcat
     6721 catcgatctc ggttccgcgt cggggactct cgtcaatggc gagaagatca acaagcggaa
     6781 gctgcaaacc ggtgatcagg tgcaagtcgg caacaagcgg atgcacatcg acatcatcac
     6841 cggaacggtg gcagtggcgt cgagtccgcc cgtggcgatg gcagccggcg gcggacgtgc
     6901 gacgccagtc gtcgacgaac gcgcgatgcg agccatgctg gacgaggagt ccgagccacg
     6961 agtcaaggca acggttactg ccagcaaggc tgacgaggaa tcgggaccta agttcaatcc
     7021 gttcgaggtt ggagccaagc gcttgtcgcg caccccattt gggaacagca gagaagaaga
     7081 cggtccggag tacggcctcg tcgcgaccac cgcggcgttg ccggctcacg aaatcgaaac
     7141 cgatgagcga gcgatcgaag tcgccgtgct ctggggcgac cggaacgttc tgaacgtcga
     7201 ctacctcacc ccgcctcgcg actattacgt cggcgagccc aacggcgaag acgtcgacta
     7261 tgtcctcgga cacgatgcaa tcggtgtgga tcgcatgccc ctttgcatcg cgcagggcgc
     7321 gggtgtcacc gccatcgtcc ccgacggagc caaaggcgaa gccacgatcg gcgaccagac
     7381 gcgttcgttc gatttgatgc gcggccaagg agatttcagg cccagctcta ccgtggcagg
     7441 ggccatggag ttccccgtgc ccgagggtgg ctcggttcga atgaagcacc gagacttcac
     7501 cttcatcatc aaagacgtga aggccggcaa gaaggtcgcc ggccatgcga agatggacat
     7561 ccgtccgatg atctacgtgg ccggtgcggc gctcttcgcg agcctcgtgc tcactatgtt
     7621 ctacttccaa ccgccacgcc cgagaggcct ttcgtcagac ttgctggcga cagactcgcg
     7681 cctgatccag ttcctcatgg agcccgagga gctcgaggag cccgaacccg agtggctcaa
     7741 cggccaggat tcctctggcg ggggcacggg caaggcacac gaaggcgagc agggccagat
     7801 gggcgacgag aaggccgaga agaccaagaa caagtacggc attcagggac cccaagacaa
     7861 tccagacccg gtcatggcgc gcgaacaagc ggaagaagaa gcacgcaccg ctggcatcat
     7921 cggaacgctg gcctcgatgc agggctcatt caattctccg acttccccct acggcgccga
     7981 ttttgcgctc ggtaacgatc cgagtagcgc gcttggtgcg atgacgggca acgacatcgg
     8041 ccagaacttc ggatttggcg gcctcggctt gcgcggcacc ggccgtggtg gcggcggtct
     8101 cggacagggc accatcggca tggggaactt tggtacgatc ggtcgcggtg gcggccgggg
     8161 tgacggcgaa ggcggctacg gtcgtggcgt tggcggattc cgcggtcgct cggcgggcgt
     8221 gcctcaagtc cgaagcggcg gtgcagaggt tcgaggctcg ctctcgaagg aagtcatccg
     8281 gcgcgtggtc caacgccaca tcaacgaggt caagttctgc tacgagcaac agctcaacgc
     8341 gcgtcctgat ctgcaagggc gtgtgacgac caagttcgtc atctcgccga cgggttcggt
     8401 tcagtcggcg atggtcggcg actcgacctt gcgaaacgag gcggtcgaga gctgcatcgt
     8461 tcgcgcggtt cgcaggtgga ccttcccagc tccggatggc ggcggtgtgg ttgtcgtcaa
     8521 ctacccgttc ctgctcgatg cagcgggcca gtgacgctca gggcgtcaga cgaaaagcga
     8581 gattcccctc tgatatccct gcttttcagg gcctgctccg gcgttcaggg aactggggat
     8641 agtctgaaac tggccaagaa cgacgaaaca gctgaaaaag agctgtgttt actgttgttg
     8701 gccagggaag taatgctgta ggattgaagt gcttgtgaat ttccaagcct aggaggcaag
     8761 catgcgagct catagtttcg gggtagtgct ggtgggtcta atcctgatgg gcgggaactg
     8821 ttctcgcgcc cgtgtggaat cgatgaacga gatgaatgcc ggcgtcgagc tcgccgagat
     8881 gaaacggttc aacgaagccg cgcaaagcct ccagaaggct gccgtgctcg atccaaccaa
     8941 tcaccaggcc tacttcaacc tagccatcgt cgagatcgag ctgcgctcct tctcggccgc
     9001 acgcgaagcg ctcgagcggg cgatcgtggc caaaccggat gtggccggat atcacgagaa
     9061 gctcggcacc gtgttgatgg agctcgagga ttggaagggg gcgcaggtcg cgttcaccga
     9121 ggcgctcgag ctcgatccat ccttgttcaa ggcgcacttc aagttggggc gctgcagtga
     9181 agagttggaa gacgcgcaga atgcgctcta ccagtacacg gatgcgatca agaccgggcc
     9241 gcggttcttg ccgtcttact cggcgcttgg ccgtttgtac gccgatcttc gttacccgga
     9301 tcaagcggcc caagtcctca gggaggcgct caaagtcgcg atgccggcca ccgaagagga
     9361 ggccaacatc caccatcttc tgggcacagt ctatcaacag caacgcaagt acgatgaggc
     9421 gattgccgag ttccaggcgg cgcttggaat cgtgccgggc atgagcgacg cgttgttttc
     9481 gctcggctgg acttatgcac ttcagggcaa cgatgaggaa gcgaagcgct acctcgacaa
     9541 gtacgtgatg atggcgggca cggatgcgcc tgagcactac gtcaaagctg cgcgagaccg
     9601 tctcgcggaa ttgagaaccg aatcgccgta agggggctgg ggcaagatgt cggaagaaca
     9661 atcgggggat gaggttcgtg agctgaccgc gggcgaagtt gccgagctcg aaaagctcga
     9721 cgacgcgatc gcgaagttcg aacaacagaa gcgatggtcg gatatgatcc gctccatcct
     9781 caagaaggcc gcgctgattc agttgccggc cgagaaggtg gagctgttcc gccaggcggg
     9841 cacgctttac ctcgaacgtt cgagcaatca ggccgaggcg atcaagtgct tcgaggagct
     9901 tctcgagtac gaggcgcacg acgtcgaggc gatccagcgt ctcaaggaga tgtacgagaa
     9961 gcggcgggac tgggagaagc tcatccgcac catgcagcga gaagtcgagc tcctgcctga
    10021 agcggatcag ctcattgcct acgccgagat ggccgacctg gcgacgcagc gccttcgcaa
    10081 gccggaggtc tgcatcgagc tttggcagaa ggtgctcgac gctgatcccg agaatccaag
    10141 cgcgctcgag aatctggcgc aactctacga gcgagcccgg gaatgggagc cgctcgccga
    10201 ggtcctggag actctggtcg ccgcagactc ggacgttact tcgatgaagc agcagcttca
    10261 gaagctcgga atgatctatg ccgacaagat cggcaacgac gaaggcgcca tcagggcctt
    10321 cacgaagctg ctggagctag acccctcgga tcgccgggcg caggaacagc tcaagcgtcg
    10381 ctatgtctcc ctgggcgcct gggacgagct agaggctttc tacgccgcca atgaacaatg
    10441 ggatgagctc atccgcatcg tggaacgaca ggccgaagct caagagaccg ctcctgagga
    10501 gcgcattccg ctcctgttca gggccgcccg tctctgggaa gagatgaaag ggaagcccga
    10561 cagggctgcg cgcgtctacg agaagatcct cggcgccgac ccggacaatg tccaggccgc
    10621 agaagcgctc acaccgatct acgaagaggc acacgatgcc cggaagctcg tgtcggttct
    10681 ggaggtccgt ttcgcgcacg cgtcggacga tgacgccaag gtcgagcttc tgctgaaaat
    10741 cggacaggtg ttcgaggaac gtctgaggca gccggaagtc gcattcgagc gctacttgga
    10801 ggcgttcgcg cttgctccga cgcatgacga ggctcgcatc gacgtcgagc gcctcgccga
    10861 ggagatgaac ggctgggatc gcttggtgtc tggattcgag tccgcaatcg atgcggtgtc
    10921 ggatccacat caggcagtgc cgattcgcct cagctacgga cagactttgg cgcagctcga
    10981 tcggatcgat gacgccatcg aacagtacca gctcgtttac gagctcgaaa gcaacaacct
    11041 cgccgcgatc actgcgctcg gggagctcta cgttcgaacc gagagattcc gcgatctgct
    11101 ggacatctac gagcgccgaa tcgagctcga ggaggatccg gaggctcgtc tcgaaatcgc
    11161 gtaccggcgc gccaagctgt ttgcccaggc gttgaacgcc ccggcaccgg cgatcgaggc
    11221 cttcgaaggc atcatcgacg aatacggtgt ggagcaaggc gatgcctgcc gtgagctcga
    11281 gcgactctat gaagccgagt cgcgatggca agatctcgca caacttctcg agcgccgggt
    11341 cgaagctcgt cccgagagcc atgaagagct tgcagccctg aaattcagac tggctcgcgt
    11401 gtgcgaatcg caactcgacg acaagctgcg agcggtcgag ctctaccgcg aggtgctttc
    11461 gattctcgct gagcacgacg gagcccgagc gcgactcgaa gccctcctcg acgacgacga
    11521 ggtcgcggcc gaggcgtcac gcgttctcga gcctgtttac gagatgagtg atgactggaa
    11581 tcactacatc aacgcacttc gcgtgcaaca ccgtttcagc acgctgcccc gcgagaagct
    11641 tcagctgctt acccgcgtcg ccgacacata ttttcagcac attggcgatg ctcagcgggg
    11701 cttcgtatcg ctctcagagg ccttccgtga gatgccgtcc gatctgggca ccctgcagcg
    11761 cctcgagatg ctcgcgaccg agcagggctt gttcgaggca gtggccgatc tcaccgggga
    11821 cctcgcggcg aacgtctcgg acgtttcgct gagccgatct ctttggatca agactgccga
    11881 gatccacgaa tcgaggctcg acgacactga ggccgccgta tcggcctacc gaaaagtcct
    11941 cgaaacggat ccaggcgact tcgaggtgct cggcgcgctc gatgtgctct accgcggcgc
    12001 ggagcgttgg cacgaggtcc tgggcgttct tcgggcccga gcagaagcat acgcggaccc
    12061 agacgaaaag atcggcgttc tggccgaaat ggcccgaatc catgccgacc tgctcgacga
    12121 gcaagagcca gccatcgcac ttcagaacga gattttggag ctcgacccga gcaaccgcgg
    12181 cgctcttggg acgctcgacg atctgctcgc gcggcagggc ctctggagtg atctcgccga
    12241 caacgtcgaa cggcagctat cgatgacgga cgatccgcag gagcaaacgg cgcttctgct
    12301 tcgcctcgcc gagatccgcg agcagcggat gggctcgatc gattcagcga tcgagatcta
    12361 ccgcgacgtc ttgacgaacg atgcgagcaa tccggccgca ctcggcgcac tggaacgcct
    12421 gctcgagtcg gccgaatacc aggctgctgt cgcagacatc ctggagccga tctacaagaa
    12481 cggtggagct tcagctcgac tcgtcgacgt gtacgaaatt caagcccacc acgccactgc
    12541 gccagagcgc cggcttgggc tgttccaaga gatcgcaaag ctgcaggagt tctccctgag
    12601 cgacctgcgc agtgcttttg aaagctacgc gcgggcgttc cgagaagacc cagggcaccc
    12661 caaggccaaa gaagagctgg agcgtctgag cgccgcggtt ggggcgtccg acgcgctcgc
    12721 gagcgtgtac gaggaagtcg tcacgagctt ggacgatccg cagctctcta ccgcgcttct
    12781 ccttcgcgca gccgaaatcc gtgaggatga cctgggtcag ctagaccgcg cggtggagca
    12841 ttatcagaac gtcctcgaaa tcgacgaaga gcaccggtac tcggccgatg cgctggagcg
    12901 cctgttccag cagaccgagc gctacgaaga cctggcttcc atttaccggc gcaaggcgcg
    12961 gatgttgccg tctcccagtg accagaggga ccaactcgtc aaagctgccg cgatctacga
    13021 agatattctc gaaaagccgg ctgaggcgat tgccgtgcat cagcaggtgc tcgagatcga
    13081 cccgacggac atcgaatcgc tcgaccgttt gatcgagttg gagctggggc tcggtaaatg
    13141 gagcgctttg ctccagacct acgaacgcaa ggccgaggtg gtttccgacc cagacgcgcg
    13201 aaaggagatt ttcgcggcga tgggccgggt tcacgaggct caactcgacg acggagacaa
    13261 ggcaatcgac gcgtatcagc gcgtcctcga gatcgacccg gacgacgttc aggcgctccg
    13321 tcatttggac gcactccacg agagcatgtc tcagtggcga gatctgttgc aggttttgga
    13381 gcgccaagcc gagctggcga cagatgagga tctgcgcatc gagctttgct tccggatcgg
    13441 tgagctctgg cggcgccatc tcgaggatcc caagcaggcg atcgaaatct accgcgacat
    13501 tctggctgag gcccccgaac atcaaagaac gctcgatgcc ttgaccgcga tgactcgcga
    13561 cgcagtggaa ccattggctg cggcggaggc gctggagcag gtctacctag ctgctggtga
    13621 gtatcgaaag ctcgccgagg ttcgcgaggt gcaagttcga ttcaccgaag aaccggccga
    13681 gtgcgtggag atgcttcagc agctcgccga aatctacgaa attcaactcg aggatccgcc
    13741 ttcggccttt gccgccttcg tccgtgcgct tccgctcgac acgcgcaacg agctgaccct
    13801 ttcgtcgctg gaacgcctcg cggagcagac tggcaattgg catgaagctg cttctcgtta
    13861 cgatgcggaa gttcagcgaa tgcgggaaca atcacccgca gacgtggtcc acctggccaa
    13921 gcgctctgcc gtgatcaacg aggtgcagct cgacgatgtg gacgcggcga tctcgcgcta
    13981 tcagttcgtc gccgacgtcg atccgagcga cgtgacggcc atcgaagcgc tcgaccgact
    14041 ctacgagcag accgaacgtt gggacgacct ggccgcgacg cttcgaatgg aggcagcgat
    14101 tgccagcacg cccgaggcgg ttctcaatct gcagttccgg acggggcaag tcttccaaaa
    14161 tcagctcggt aagatggatt ccgccatcga ccagtaccgc gagatcctcg ccgctgcccc
    14221 cgagtatgcg ccggccgtct cggcactcga ggatctgttc tcgagcggtg tgcagcccat
    14281 ggcaattggc gaagtgctcg agccgctcta ccgcatgcag ggcaattggg atcggctgct
    14341 caacgtgcag gaggtgcaag tcgagcatca gaaagacccg atcgagcgcg tcgtcatgat
    14401 gcaccgactg gcggagatcg cggaggacaa gacttcggac cacggtcgcg ccttcgagtg
    14461 gatgcagaag gcgctgctcg aagaccctag ccacgaccac tcattgggtg aggcagagcg
    14521 cctttcggcg atcgtggacg gctggaccga gcttgcgact acctacgcca atggcattca
    14581 gcacgccgga gacccgctca acaaggcgga gctcggacgc cggctcgcca aggtctacga
    14641 gaacgagctc cacgatctcg aacgcgcgga ggagacctat cgattcgttc ttggcgtgga
    14701 agcccacgac gacaaggcgc tgaaggcgct cgatcgaatt tacacggagc acggcgcata
    14761 ccgcgcgctc tgcgaagtgc tgcgcaagcg aatcgaaggt tgcaacaaca gctcggagct
    14821 catcgatctt cactatcggc tgggcaccgt gctcgaacaa cgccttggat gcaccgaaga
    14881 cgccatcgag gtgttcacca aactgctcga cgagtacgac tccgagcatt ctcagacgat
    14941 ccatgcgctc gaaggcatct atgccaatct cgggcgctgg ccggagctat ttgcagcatt
    15001 cgagaaagag ctgagcatcg cgctcggcga ttcccagcag gccgacgtgt ttgcgcgcat
    15061 ggcaaagctc tcgacgaacc ggctcagcga cccggagaag gcgatcggcc tctggcagcg
    15121 cgttctcgac ctgcgcggag aggatcccga ggcgctcaac gcgctcggtg acatctatgc
    15181 gtcgcagggc aattggcgcg acctcgtcga cgtgctcgag cgagaggctg gcatcgccga
    15241 cgacgatgcg ttccggatcc aagtgtactc cgacctcgcg agggtctggt acgagaagct
    15301 cgagcgcgag cgaaacgcga tcgagaactg ggaacgcgta ctcgacatcg atccgggcaa
    15361 cgcgtacgcg ctgtttagca tcgcggagat ccagcgggcg ggggggcaat cccgggagcg
    15421 ggccgacacc cttcaccgcg tcatcgacgt cggtgcggcg accctcgagg acgccgatct
    15481 gatcaccgtt tacatgcagc ttgggcacat ctatgcaaac gagctgaatc aaccgggcga
    15541 cgccgtcgag gcctaccgca acgtactgac gatcgatccg cgcttcttcg aagcgctgga
    15601 ctccctcgag ctgattcacc gcaaagaggg tgattgggaa gaggcgatcg gggtgatgga
    15661 gcagcgcgcg caggccctcg ccgatccaca agagaagatt cgccagctca tcgcagtcac
    15721 cgagctttgg gagaccgagc tgggcaacaa ggaaggcgct gtgggtgcct tcggacgcat
    15781 cctcgaagtc gacgcgatgc acgagtatgc attctcggag tcgcagtcgc tgctgaggaa
    15841 tgcggagcgc tgggacgagc tcatcgacct ctacgtgacg cgcatcgaag ctacggaaag
    15901 ccggaaagag cgcatcgagc tgttgcgcgc ggccgcgagg gtctacgagg aacacctcga
    15961 cgatggcgag caggcctatc acacgctcta catcgcgtgg accgaggact ttgccaacaa
    16021 gaacaccgcc gtcgacctcg agcgagtaac cgcagccaac gacaagtgga acgagcttct
    16081 cacttcagcc aatcaggtct tgcaagacgg tcaggaaccg gagacacaga tcgcgatctg
    16141 cctctgctgc gccaagtggt acggccagga cctcggacat cccgagtacg ccattccgta
    16201 ctacgagcag attcgcgcgc tcgacccgag caacgcgaag gcttggtggc agctaggcga
    16261 cctctacgag acgacccatc agtgggacaa gctcgctcag gcgctcgcca agctggtcga
    16321 gctcacggac gacgacgacg tgaaggccaa tacctacgtt cgtatgggag atctggcgga
    16381 tgaccgcttt gccatgcgtc aagaggctga ggactactac cgaagggcgc ttcagctcca
    16441 ggacaagcac gtgggggccc tcgaggcgct cgagaggatc taccgggagg acggccgatc
    16501 caacaagctg ctcaacatac tcgaacgaaa ggcggaagcc gtcgaggatg tcgagagcgt
    16561 cgtctcggcg ctgttgcagg tggccgaggc ctacgaggaa cgattcgatg acccgaaagc
    16621 tgcgaccgaa aattatgaac gggcggccga gctttcgccc acggatctca ctgcgcttcg
    16681 aggtttggag cgtttgtata cgcaagatgg acgctatcag gatctgattg gcattctcga
    16741 gcgtgagtac gaggtcgttg caaccgagcg cgagcgcatc gagattttga tgcgactcgc
    16801 caagatgtgg gaagaggagt tccgcaaacc ggagaacgcg gccgagcggc tggagcaggt
    16861 gctggacatc gacccgcttc atgatgaagc gctttgcggc ctggaggagc tctaccggac
    16921 catgcagagc tgggacgacc tggttcggac ctacgaccgc catgcgagcg cgacggccga
    16981 taccgaggaa cgggtccgga ttttccgaga gcttgggcag gtctacgcga ctcagaagga
    17041 agatgtcgag caggcgatcg acagttacct caacgcgctc aacctcaacg agcaggatct
    17101 cttcagtctt caggcgttga cgaagctcta cgagcgcaag ggcgactacg cggcggccct
    17161 cgacatgatg ggccagcttg tgggggtctc cgacgatccc agcgatcaag tggcgctcaa
    17221 acaccgaatg ggacgcatcc tcgaccaaga gcttggggac cgcgtgtcgg ctctggagca
    17281 cttccaaacc gccatcgatt tggatccggc acatctcccg tcgctcgagg cgatgcggag
    17341 catttacgtc gactcgggtg attgggctgc cgctgccaag accctctgcc tagaggtgga
    17401 gcacaccgaa aaggatcgga ttcgggccgt gcggctcgtc gagcttgggc gcattcgtgc
    17461 cgagcggctc gacgacgagg acggcgcggt ggaagccttc gaggctgcaa tcgaggccga
    17521 tgccgacaac gaagacgcga tgttgccgct cgtcgatgcg tacgtgagcg cggggcgcca
    17581 cgagaacgcg ttcccgcttc tgcgaaccct cgtacaacgc tcggacaaac gcgagcacga
    17641 ggagcagcac cgccttgcct cgatgttggg tgaaaccgct acgatgctag ggcacgccga
    17701 cgaggccgtg agcgcttacg agcgcgcgta cgagctcgat agcaacgatt cggtcacgct
    17761 caaggggctg gcgggagcac acttccgagc ggaacgctgg gacgatgctt tcaagtatca
    17821 ccagcttctt ctggttcagc accgcgatgc gctcgggcga gacgagatta ccgagatctt
    17881 ctaccggctc ggtgtcgtca agcgagagca aggcgaagag aagaaggcgc ggaacatgtt
    17941 cgacaaggcc ctcgaagagg acacgcattt cgtgcccgcc ctcgaggcca tggtcgcctt
    18001 gcagaccgcc gacggcgact ggagctcggt cgtcgagtac aagaagcgaa tactggacgt
    18061 ggccgacgag ccctctgttc gggcggcgat tctcgtcgag ctcggagacc tctggcacga
    18121 aaagctcgga gaaccgggct cggcagccga ggcatacacc gagtcactgg acctcgaacc
    18181 cgaaaatcac gtcgtgcttc acaagctcct cgtggcctat caggctgcgg agcgctggtc
    18241 ggaggccatc gacgtcatcg agcgcgttcg tggaattgaa gagcgggacg aggccaaggc
    18301 caagtacacc tacacgatcg gggtcattct acgcgacaag ctagaagacg aagatgcggc
    18361 actcgagcga ttcaacgatg cactcgacat cgattgcact cagctgaagc cattcgaggc
    18421 ggtcaacaag ctgttgaacg agcggaagga ctggaaggca ctcgagcgag cgtaccgcaa
    18481 gatgcttcat cgcatcatca accagggcaa cacggacctc gagcacaacc tctggcacac
    18541 gctcggtatc atctatcgag accgccagcg taacttcgat gctgccgcgg agtcgttcca
    18601 gatggccgcc ggcctcaagc ccgacgaccc cgtcgggcac cagatattgg ccgagctcta
    18661 cgcgaccatg ccggaaaaga ccgagcaggc tatcgtcgag caccagtggc ttctcggcgc
    18721 tgacccgaac cggctcgatt cgtatcgggc cctctacaag ctctacttcg atgcccgcgc
    18781 ttacgacaaa gcttggtgca tcgcgcgcac cctgagctac ttcgaaaagg cggacgaaga
    18841 gcagaggaag ttctaccagc agtacaagca ggatcgggag atccagccga agacgcggct
    18901 gagcaatgaa gcctgggtga aggagctgat gcacgccgat caggacctct acatctccaa
    18961 gatcatggag gtgatagccc ctgcagttcg agcttcgatg gctatgaccg acaagaagct
    19021 caacttgaac aagcgcaagc ccgaggacct cgccaatccg agcctcgcgc tcgcccggca
    19081 gttcaagctt gcgcaggacg tcctcaacgt gacgattccg gttcgcctat tcatccagaa
    19141 ggagctttcc ggcgcgctgc gggacgaacc gaggagtaat ccgcctgcga tcatcaccgg
    19201 gtacacgctt ctctcgggct accggcctgt ggatctggcg ttcgtatgcg gccgtcacct
    19261 cacatattat cgcggtgagc atttcatccg gacgatgtac aactcgcaca ccgacttgag
    19321 gactctcttg ctagccgcca tgcgtgtggt cggcatggga ggcggggacg caagcgtcga
    19381 gtccacagcc aagcagttgg cgaagtacat ggaccagtcg cacctggacg tgctcaagca
    19441 ggtcgttcgc aagttcgtcg atgctggagg gcaggcggat atcaagcgtt ggatgcaggc
    19501 ggtcgaggtt acggcgcttc gcgccgggct actcctctgc gacgacgtgg acaccgctgt
    19561 ccgcatgacg cagcagatcc cgtccgaatc cacggccgac ctcgctccgc gcgacaaggt
    19621 caaggagatc gtgctctaca gcgtttcgga gagctacttc cgccttcgag agcagctggg
    19681 catacaaatc aaggtctaga cgagcaggcg tgcactggct ccccggatgt gacagggcat
    19741 gggcatgctc gagctggagc ttcacagcga ggcggacaca gtcgccctgg cacgcaccct
    19801 tgcagccgtg cttgcgcccg gggacgtgat cggtctcgaa ggggggctcg gcgctggcaa
    19861 gacgaccttc acgcgcgccg ctgttcacgg gctcggcgtg ccggcagaga cggcggtcac
    19921 cagtcccacc tttgctctgc tgcatcagta ccaggggcgt ttaccaatcg cgcatgcgga
    19981 cttctaccga ctgggagacg aagccgagct cgaagagcta ggagtcgatg agctcctcga
    20041 ggagggtggc gtgctcttcg tggagtgggg aaagaagttt cctgcgatgg gggctcggac
    20101 ggcgctttgg gtcgagctcg agatcttatc cgacctcgcc cgccgggccc gttttaggcc
    20161 ggagagcgca cgtggtgatg cgatcatcag cgcactcagc aagcgaggtt cggacgaaga
    20221 ccttcccagt tgcggcccct agggcgctct ggtaagcccg cgcgaacgcc caggttgaac
    20281 tgattgcatg ccccgccgcg acgatattga aagcatccta ctgattgggt cggggccgat
    20341 cgtgatcggt caggcctgcg agttcgatta ttccgggact cagggcgcca aggcgctcaa
    20401 agaagagggt taccgaatca tcctcgtcaa ctcgaacccg gcgacgatca tgaccgaccc
    20461 ggagttcgcg gaccgcacat acgtcgaacc gctcaccccg gagatcctcg aaacgatcat
    20521 cgaacgggag aagcccgatg ccgtgcttcc gaccctcggt gggcagacag cgctgaacct
    20581 cgccttgtcg ctcgccgacc ggggtgtgct cgaaaagcac ggggtcgagc tcatcggcgc
    20641 gaacgtcgag gccatacgca aggccgaaga gcgcgagctg ttcaaggcgg ccatggacaa
    20701 gatcggactc gaaagtgcgc gagccgaggt cgctcggacc gtcgatcatg cccacgagat
    20761 cgcagagcgg atcgggtttc cgatcatctt gcgtccgtcg ctgacgatgg gcggctcggg
    20821 cggtggcgtc gcctacaaca aggcggagtt cgacacggcg attcgctggg ccttccagtg
    20881 cagccccgtg cacgagtgcc tcgtcgagga aagcctgctc ggttggaagg agtacgaact
    20941 cgaggtcatc cgggacaagg cggacaactt cggggtgatt tgcacgatcg agaacttcga
    21001 cccgatgggg gtccacaccg gcgattcgat cacggtggcg ccggccatga cgctcaccga
    21061 ccgcgagtac cagcgtctgc gagacgccgc ccgggcgatc atcacggaga tcggcgtcga
    21121 gacgggcggc tccaacatcc agttttcggt caacccagcg gacggcaggg tgatcgtcat
    21181 cgagatgaac ccgcgcgtct cgcgatccag cgcgctcgcg tcgaaggcca ccggctatcc
    21241 gatcgccaag atcgcggcca agctcgccgt gggctatcac ctggacgagc ttcgcaacga
    21301 catcacgggc accagcgctg cgttcgagcc cacgatcgat tacgtcgtca ccaagattcc
    21361 gcggttcgcg ttcgagaagt tcgccggcgc tgatccgcgc ctcacgacgc aaatgaagtc
    21421 ggttggcgag gcgatggcga tcggccgcac gttcaaggag tccctccaaa aggcggcgcg
    21481 ctcgctcgaa acgggaagag acgggttgac ctcgcttctc aagaaggtgg actacaggga
    21541 cctcgccgct caggtgagac ggctcatcga gacgggcgaa gcgatcaaca ccgccaagcc
    21601 gatgctcgcc gacctgcccg atgcgaccga cgccgagctt cgagagtcga ttctagatct
    21661 cgtgcgcacc ccgctcgccg atcgcctttg gtatctcgcc gacggtctgc gagtagggct
    21721 taccgtggaa gacctccatg gagcgactca gatcgatgtt tggttcctcg agcaaatgcg
    21781 ccagatcctc gccttcgagg ggaccttgaa ggagtcgacg ggcaaagcgc tcggcgaggg
    21841 gctccttcgc gacgccaagg ctctgggttt cagtgatgcc caaatcgcga cgctccgtgg
    21901 tgagacgagc aagtccgtgc gcgccgagcg cgcggcgaag ggcgttgagc cggtcttctc
    21961 tcgagtcgac acatgcgctg cggaatttcc tgcgctcacc ccttacatgt attcgacctg
    22021 gggactcgac agcgaggctc gagtcggcgg ggacaagaag aaggtgatga ttctcggtgg
    22081 agggcccaac cggatcgggc aggggatcga gttcgactac tgctgcgtcc acgcctcctt
    22141 cgccctcaaa gagatgggct tcgagacgat catggtgaac tgcaaccccg agacggtgtc
    22201 cactgactac gacacgtcgg acaggttgta cttcgagcca ctcaccttcg agaatgtgat
    22261 gcacatcgtc gaggtcgaga aaccagacgg catcatcgtg cagtttggcg ggcagacacc
    22321 cctgaagctc gccgtgccct tgtcgcgtga gggcgtgccc attcttggaa ccacggccga
    22381 tgccatcgac cgcgctgagg atcgagagcg gttcgaggag ttgctgacga agctcggtct
    22441 cgaccgtccc cgtggcggta tcgccaaggg cgccaaggac gcattcgcag tcgccgaacg
    22501 cattggattc ccagtcgtcg tgaggccaag ctacgtgctt ggcggtcggg ccatggaaat
    22561 cgttcactca caagaggagc tcgcgcgata tatgacggtt gccttagaag ccgtcgccga
    22621 cgcggaagcc cagaccatct tggtcgacga gttcttgaaa gatgcgatcg aggtcgacgt
    22681 agactgtgtc tcagacggcc atgacgtcgt cattggtggc ctgctccagc acatcgaaga
    22741 ggcggggatt cactccggtg actcctccat gtcgttgccc gctcacgcgc tcccggacga
    22801 cgtgctcgcc acgctccggc agtcgacgcg cgcgcttgct ttggagctcg gggtcgtcgg
    22861 actcatgaac gtgcagtttg ccgttcgggg tagcgcggtg ttcgtcatcg aggtcaatcc
    22921 cagggcgtct cgaacgatcc ccttcatcag caaagcgatc ggcgttccac tcgcaaagat
    22981 cgctgccaag gtcatggccg gttcgaccct tcgggagctc ggcttcacgc gggaaatcgt
    23041 gaccgaacac atcgccgtca aagaatcggt cttcccattc accaaattcc caggcgtcga
    23101 cacgatcctc agccccgaga tgcgctccac gggtgaggtg atgggaatcg ccgacaattt
    23161 ccccgaagcg ttctacaagg cgctgctctc cgcatacacc gagctgcctg acgccggaaa
    23221 agtgttcatc agcgtcaaag agagcgacaa acctgcggct gcccagctcg cgcaccgcct
    23281 caagggcttg ggcttcgaaa tcgtcgccac gtcggggacg cgaaacgtga tcgccgaggc
    23341 cggggtcgac gctgagaacg tgcacaaggt gggagagggc cggccgcaca tcgtcgacat
    23401 tattcagaac ggtgaaatca acttggtgat caacaccacc gccggagcaa aggcgattcg
    23461 ggacagccgg tcgatgcgtc gccaaaccgt cctgagcgac gtgccgtatt tcaccacgat
    23521 cgccgctgcg gtcgctgcgg tcgatgccat cgaagcgcgc cgccgccggc ctctttcggt
    23581 ctgctcgctc caggagtacc acgcgcgcaa ccgcgaattg gccaccctcc tgccgccgag
    23641 cgactagcgt tgggttgcct catctggggc cagacactac gaggagcgca tggaacgggt
    23701 tccgatgacc ccagctgggc atgcagccct taccgctgag ctcaacgccc tcaaggccga
    23761 gcgaccgaag atctcccagg agatcggcgt tgcgcgtgac cacggcgatc tcaaagagaa
    23821 tgccgagtat cacgccgcca aagagaagca gggcatgtgc gaagcgcgta tcgcagagat
    23881 cgaggacaag ctttcgcgcg ccgaggtcat cgatccctcg acattgagcg gcgaaaaggt
    23941 gaagttcggc gccatcgtcg agctcgagga catggacaac ggcaagatgg tcacctaccg
    24001 cctggtcgga ccggacgagt ccgacatcaa ggagggcaaa atctccatta cttcacccgt
    24061 ggcccgcgcg ttggttggca gggaagtggg cgacgaggtg cgggtcgaga cgccgggtgg
    24121 ccttcgccac tacgaagtga gcgacgttcg ctggggctga aggggccacg gctctggggg
    24181 gaagggcgcc agtgcccaca ctgctcgatc atcctgtcga aacgctcccg gggatcggat
    24241 ccacgacggg cgcccatctg cgggcacgcg gcttcgaatc ggtgggggat cttctctggc
    24301 ttctaccacg cggatacgac gaccagcggg ctccgacccc gattcatgag ctccgttgcg
    24361 gcgagtacgc ggtcgtcgaa ggcctggttc gcagggcgcg atcctttccc cgcggcggtc
    24421 gtctcggatt cgaggtccga ctggacgccc ctgaggcgcc ggctgaggtc gacggctacc
    24481 gagagttgaa gctggtctgg tttcgagcga ttccggggct cgctcgtcgt ttcgccgagg
    24541 gcgagagggt gcgggtagcg ggtaaggtca ccgattatcg cggcacggcg accatggcgc
    24601 atccagagtc gctgcccgcc gacgtgcgtg gagcgatcga acctcgctat cccgaggtgc
    24661 ccggtgtccg gcgcaagata cttcgacgcg ctgtgcgagc cgcggtggat cgcgcggtcg
    24721 ccgaggttcc cgatctcatc ccagcggcgc tccgcgccga ggtcggcgca tgcaccgtgg
    24781 cagaggcgct ccgttcgatt cacgttcccg atccggaagc gttcgatgcc gtcgccaacg
    24841 ccgagatgct aggcccggcc caccatcggc ttgcgctcga agagctcgtg ctctgggagc
    24901 tcgcgcttcg tctgcgtcga gcgtctgagc gaggaggact ttcgcgcgcc ttcgaaatcg
    24961 gggcttcagt gtccgcggcc tcaggtgcat tcccattcga gttgaccgca gcgcagaggg
    25021 cggccgtgcg agacgtgagc gcagcactct gtagcgaaac gcccatgcgt cgtcttctcc
    25081 agggcgacgt gggatgtgga aagaccgcgg tggcgatggt cgcctgcgcg caagtcgctg
    25141 caggcggcgc gcaagctgtg ttcttggccc ccaccgagct ccttgcagac cagcacgcag
    25201 agactcttcg accgacggca gaaaaactag gtttgcgatt aggcgtgctc acaggcgccc
    25261 tcaacaagga ccatcgacgg tccatgctcg atcgcttggc gaccggagcc gtcgatttgg
    25321 tcgtcggaac gcacgcgctc ctgagcggag acgttcgatt cgcagagctc ggattggtga
    25381 tcgtggacga gcaacatcgc tttggcgtcg cgcaacgttt gcgactcggt tcgcgtggtt
    25441 cgacgcgcac ccctcatctt ctcgtcatga ccgcgacgcc gattccgcga agccttgcgc
    25501 tcgttttgta cgcggggctc gagctcacca cgatcgatgc caagccgccc ggacgggttc
    25561 cggtgaccac caagatgctc tcccgcctga agcgcgatgc ggtgtaccgt caaatcgaac
    25621 gcgcgctcga ggccgggggt cgagccttcg tcgtgtgccc ggccatctcg acctccgaag
    25681 agctggtcgg tgtggaccag gttttcaccg agatggaagc gcgattcggg agcgctcggg
    25741 tcggccggat tcatgggaag ctttccggcg acgaacgccg cgtcctgatg cgccagttct
    25801 cggagggcga gctcgatgtc ttggtgggca cgaccgtctt ggaagtcggc gtcgatgtgc
    25861 ccaaggcgaa catcatggtc atcgagcagg ccgagcgctt cgggctagcc cagctccacc
    25921 agcttcgagg gcgcgtcgga cgcgcgggcc aaaggagcgc ctgcttgctg atgttctccg
    25981 agccgctgag cgccgacgcc gaggcccggc ttcaagccct ttgcgagact gacgacggct
    26041 ttcggctcgc cgagcgcgat ctcgagctgc ggggtgcggg tcaccttttc ggataccgcc
    26101 agtcgggagc gagcgggctg cagtttgcga atctcgcgag ggatcagagc ctgcttcagc
    26161 gctcgggcga tctggcggac cgcatcatcg gagaggatcc gggtctctcg aagccggagc
    26221 acgcggcagc ccgggcagcg atagagcgct gggaaagctg cggggcggta cgcgaagagg
    26281 cgggctgacg gcctggcgcg cccacacagg ctttcttgac gccttcgcgg gacccaggta
    26341 gttcccaatc atgccgctcg ttcaccatgc gcagcgcgaa gtcacgctca agctcgtcta
    26401 ctacggcgtc gggctggggg gcaagacgac caatctcgaa accatccacg cgcagacccg
    26461 tccggaggcc cgtggcaagc tggtgtcggt caacacagaa gcggagcgga cgctcttcct
    26521 cgactttctc cccctgcagc tcgggacctt ccgcgggtac accgtgcgtc ttcatctcat
    26581 ttccgttccc gggcaaatcg ctcaggactc cactcgccag atggtgctcc ggcacgtcga
    26641 tggcgtcgtt ctcgtagtcg acagccaagc cgacgccatc gagggcaaca actacagcat
    26701 tcgcaacctc gactacaacc tccgacagca gggcgtcgat cccgatcgaa tcccgatggt
    26761 cgttcaatac aacaagcgag acctaccggg catcctcgat gtagacgagc tcgcggagcg
    26821 cctgggcgtt ccggaaggca tcccggagct cgtggcgtcc gcgcgggacg gctggggcgt
    26881 attcgagacg gtgaagtcga tcgtgcgcat ttgcatgcat caactcggtg acccgagcct
    26941 caggggcgac ggtcgcgtcg agtgcttgct cggtgagcca cgcgatcgtt tctacccaag
    27001 cggcccggtg tcgatgattc acgccctcag gcccgaggag gagctcgagc tggaagacct
    27061 cgagctcatc agcggctgag ccgatgaatc cgcctggcca agtgtccggt ggccacgatg
    27121 tccggatcat tgctccgacg atcgagatgc tcccggtacg cacgccgacg ctgcccccgg
    27181 cgacccacac gaacagcttc ttggttggca cgggtgaggc cgtgctcatc gagcccgcga
    27241 cgccgtatcg cgaggagcag gaccggatgg ccgactgggt ccgctccgcc cgattgaaag
    27301 gtgtagagcc catcgccatc ctcgcgacac accaccaccc cgaccacgtc ggtggcgcca
    27361 tggccctccg ggagcgtctc gagctcccgc tctgggcgca tgccatgacg gcgcaacgcc
    27421 tcgacggcat cgtcgaagtg gaccgcttga ttcaagacgg cgagcgcatc gagctcgacg
    27481 gcccgacgcc cacgtcgctc agcgccctgc acaccccggg tcatgcgccg ggccaccttt
    27541 gcttcaccga tgaggtcagc aacatcatga tcgccggcga catggtggcc tccgtcggga
    27601 cgatcatcgt cgagcctacg gacggcgaca tgctcctcta cctggagtcg ctcgagaaga
    27661 tgtcggcgct cgagccggcg gggcttctgc cggctcatgg agatctgatt accgagccag
    27721 aagcgattct gcgcttttac atacagcatc gattgatgcg tgagcgcaag gtgctcgaag
    27781 cgctggaaga acgcggcaag ccgtcgcggc cccgccacct ggtcgcctcg gcgtacgccg
    27841 acaccgcgaa ggcactctgg cctctagcac ttcagtcgat tgaagcgcat ctcatcaagc
    27901 tcgaacgaga aggtcgcatc gccaaagagg gcgaccgctg ggcaccgatt acgcactgac
    27961 actgaactaa cggggcacgg gcgagtgggt cctaatcgcg agcacgcccg tcgcgagccg
    28021 tcctccggcg actccgtagc gcaacacggt cgctggcgcg ggcgctgggc gctgcaccgg
    28081 cactggcact ggcacgggca cgggcacggg cactgacgct ggcactcgca ctggcacggt
    28141 cgctggcgct ggcactgact ctggcactgg gcactgacac gggcaccgac gctgctgtcg
    28201 gcttcttcgc atctctcctt gtcatcgctt gtccttggat tgggttttgg ccgcacgggt
    28261 gaggccaaaa cccaatccaa ggagtcgctc atgggaaaga agaatgttta cgagagggcg
    28321 cgagcggtcg tgcgggagct cgctccggcg ctgcagacga ttcgcgactg tgacaagtcg
    28381 ctcgccgatc agttgaagcg ggctgcacag agtctggtgc tgaacatcgc ggaggggcgg
    28441 gggagtgacc ccgggaatgc acgggctcgg ttctctacgg cttgcggatc agccaaggaa
    28501 gtccgggctg cgctcaacgt cgcgagcgac tggggttaca tcgagtcgac gaccgcagcg
    28561 cacctcgacg agaagctcga cgaggtgtgc gcgatcacct ggtgtttggg ccggagacta
    28621 tgatccgccc tgtcactgcc acagccaggt acggtccgaa gcttcgattg caccctggtg
    28681 cgcagagagg aggacggttc gggatgggca gcacagcgat tgggtcgagc ctagccggtg
    28741 ggtcgtctta gagaactggg ggcgagtggg tcccaatcgc gagcacgccc gtcgcgagcc
    28801 gtcctccggt gactccgtag cgcagcgctg ggcgctggca ctggcactgg cactggcact
    28861 ggcgctgggc gctggcactg ggcactgacg ctgggactgg cgctggcact gacgctgggc
    28921 gctgccgctg gggaccggcg ctgccacagc caggtacggt ccgaagcttc gattgcaccc
    28981 tagtgcg
//
LOCUS       DASRZF010000151         5995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2852730, whole genome shotgun sequence.
ACCESSION   DASRZF010000151 DASRZF010000000
VERSION     DASRZF010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 5995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5995
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2852730"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VFG22_06885"
     CDS             <1..663
                     /locus_tag="VFG22_06885"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416003.1"
                     /translation="PLTNQRTDRWGGSLENRARFARTVLGEIRKNVGDAFPIGIKLNS
                     ADFQRGGFTENESMQVLGMLQEDGIDLVEISGGSYESRVMLDKAENHREAFFLDYARK
                     ARAEVDIPLMVTGGFRSRPIMAEALAQGALDVVGMARPFTNDPAVAQDLLDGRSDRAA
                     DPPAIPGLGRLGGTSEAMMSVAQMALLAQGKSPTLRFGGLGAVFASLVQEGRDLFKPK
                     RS"
     gene            complement(644..1387)
                     /locus_tag="VFG22_06890"
     CDS             complement(644..1387)
                     /locus_tag="VFG22_06890"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET6416004.1"
                     /translation="MFETSTVLVLVVACFVTATISGILGMAGGVTLLGVMTALLPAPL
                     VVPLHGIVQLASNWTRTWAFRRHVRWSIFFAFMAPAVLGVAVAASIWADLKLTWFRAW
                     VGVFILLFLLWRRYKPKLRNLPIGAYAVLGLAAGLLAIFVGATGPFLAPFFLRDDFEN
                     EEVIATKAVCQTWLHLLKIPAFLALSFDYMPYAPLLLGLVVAVIGGTYFGKHLLSRIS
                     KERFVFWFQLVLALLALYLIAASLRSAWA"
     gene            1452..2102
                     /locus_tag="VFG22_06895"
     CDS             1452..2102
                     /locus_tag="VFG22_06895"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HET6416005.1"
                     /translation="MASRVLHFHFDYISPYSYLAWHQLQAFARAHELSVEPKPTLLAA
                     LLNALGHKGPGEIPPKRSYMFKDCIRAAAQQHVPFVPPYSHPFNPLASLRATLLDMDA
                     GTRQRLVTRLFDATWAESRDLGSPEVVAQICAEVGVPDARERIQDPAIKERLFAASQR
                     AIELGVFGVPTMIVDGELFWGTDSFPHLARYLAGQDPIRSEDIAAWAAIPASAHRK"
     gene            complement(2107..3156)
                     /locus_tag="VFG22_06900"
     CDS             complement(2107..3156)
                     /locus_tag="VFG22_06900"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6416006.1"
                     /translation="MLLRQLFDHETWTYTYLLADEASGEAVLIDTVIDQLERDIKLLK
                     ELGLKLMYTLDTHTHADHITGCGLLRQRLGSKAVVGANAGTAGADVLVDDGSKLKFGS
                     LELEVRATPGHTDGCVTYVTSDHKHAFTGDALFIRGTGRTDFQQGSAERLYRSIHDKI
                     FTLPDDTIIWPGHDYRGFTFTTVGEEKRLNPRVGDGKSEAEFVEIMSHLNLPAPKKLG
                     VAVPANLHCGIVPPSSMSGDPLPERGWAAIERTPDGTPEVTLAWVAENRGVIRMVDVR
                     EIDEAESDGKIEGVEVIPMGTVDEASKSWDKEEPVITICRSGRRSGRVALELEKLGFK
                     RIASMRGGMVAWQSQ"
     gene            3297..3728
                     /locus_tag="VFG22_06905"
     CDS             3297..3728
                     /locus_tag="VFG22_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HET6416007.1"
                     /translation="MESFTPIASTVGGLMIGVAAAGLLLFHGRIAGISGIAGGIFRRE
                     SGDISWRVMFIVGLFTAGAVWSQFFPLDYEVGIERSTLALIIAGLTVGIGTQLGSGCT
                     SGHGVCGIGRLSKRSTVATISFMITAAITVFVVNEVLGGSI"
     gene            3725..4198
                     /locus_tag="VFG22_06910"
     CDS             3725..4198
                     /locus_tag="VFG22_06910"
                     /inference="COORDINATES: protein motif:HMM:NF042761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HET6416008.1"
                     /translation="MKVKIGAFLGGSLFGVGLAIAGMTQPAKIIGFFDFFGSWDPSLA
                     FVMLGAIVVYTPVYRWAIRTWQRPIWAPAFSLPTREDIDARLIVGSAIFGVGWGLGGY
                     CPGPALTSVGAGSEQAFIFGAAMLIGVGAYQLFMRVRERRTGGAVDPQAAAMIDG"
     gene            4226..5614
                     /locus_tag="VFG22_06915"
     CDS             4226..5614
                     /locus_tag="VFG22_06915"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3336 domain-containing protein"
                     /protein_id="HET6416009.1"
                     /translation="MGGYTDWLRKVEEAADRAEVERWRAEPRSSVYDAKLLREQTEEL
                     TRTRETGKALELVPSLREALYRQLGELADPRLYAVTPLGTKYRVEAFYEEALACIAYL
                     ADPDQTGLSPEFTLAGFRRAAHIFGRSALLLSGGATLGFFHLGVVKALSEQGLLPNVL
                     SGASMGALIACGTGVRTDEEIKTLFEDPRVIRTDALKRLPPKRAAKERAVYDPARLAE
                     VVANNCGDYTFEEAHARTGRTVSISVSPTRERQKPRVLCHYTTPNVLMNSAAVASASI
                     PGAFPGAMLQQRGPSGEPIPYMSTERWIDGSLKGDLPMRRLSRLHNVNHFIVSQVNPH
                     VAPVRSVTGKRGIVPVVSGVILSSFRAQMTHQLELARDLTKSTPLYSTFDVAHSLAGQ
                     PYGGDIDIHPRLRPVALLRAMSNISHEELKKHIREGEYATWPLLARVRDQTCVSRALD
                     AAIDRLSAELAR"
BASE COUNT         1091 a         1859 c         1901 g         1144 t
ORIGIN      
        1 ccgttgacga accagcgcac cgatcgctgg ggaggctcgc tcgagaatcg cgcccgattc
       61 gcgcgcacgg tgctcggcga gatccgaaag aacgtcggcg atgcgttccc gataggcatc
      121 aagctcaatt ctgccgactt ccagcgcggc ggcttcacgg agaatgagtc gatgcaggtg
      181 ctcggcatgc tccaagaaga cggtatcgat ctcgtcgaga tatccggcgg cagctacgag
      241 tcccgggtca tgctcgacaa agcggagaac catcgggagg cgttctttct cgactacgcg
      301 cgcaaggctc gagccgaagt cgacattccc ttgatggtca caggtggctt tcgatcgagg
      361 ccgatcatgg ctgaagcgct cgcccagggt gcgctcgacg tggtgggcat ggcccgcccg
      421 ttcaccaacg atcctgcggt cgcgcaggac ctgctcgatg ggcgtagcga ccgagcagcc
      481 gacccgccgg ccattcccgg actcggccgc ctggggggga cctcggaggc catgatgagc
      541 gtggcgcaaa tggcgctcct cgcgcagggc aagagcccga cgctacgatt cggcggtctt
      601 ggggcggtgt tcgcttccct cgttcaagag ggcagggacc tgttcaagcc caagcgctcc
      661 tgagggacgc tgcgatcaag tacagggcga ggagcgcgag caccaactgg aaccagaaca
      721 cgaagcgttc tttggagatt cggctcaata agtgcttacc gaagtacgtg ccgccaatga
      781 cggccaccac caagcccagg agcaagggtg cgtagggcat gtagtcgaag ctcagcgcca
      841 gaaaagccgg gatcttgagc agatggagcc aggtttgaca gacggccttg gtcgcgatga
      901 cttcttcgtt ctcgaagtcg tcgcgcagga agaagggcgc tagaaacgga cccgttgctc
      961 cgacgaagat cgcgagcagc cccgccgcga gacccagaac ggcgtaagcg cctatcggaa
     1021 gatttcggag cttcggcttg tagcggcgcc agagcagaaa cagaaggatg aaaaccccga
     1081 cccaggcgcg gaaccaagtc agcttcaagt ccgcccaaat gctcgcggcc accgcaaccc
     1141 ccaggactgc gggggccatg aacgcgaaga agatcgacca gcgtacgtgt cgtcggaacg
     1201 cccaggtacg cgtccagttc gaggcgagct gaacgatgcc gtgcagcggg actacgagcg
     1261 gcgccggaag caaggcggtc atcactccga gcagggtgac tcccccagcc atccccagga
     1321 ttcccgagat tgtcgccgtg acgaaacagg cgacgaccaa cacgaggacc gtgctcgtct
     1381 cgaacatcca tgcaggctag cactgctttc cgcttgtgct tcatccgcat cgtgggtatt
     1441 cgacgagacc tatggcgtcc cgcgtccttc actttcattt cgactacatc tcgccctaca
     1501 gctatctcgc ttggcaccag cttcaggcat ttgcgcgggc gcatgagctc agcgtcgagc
     1561 cgaagccgac cttgctggcc gccttgctca atgccctggg gcacaaaggg ccgggagaga
     1621 ttcctccaaa gcggagctac atgttcaagg actgcattcg agcggcagcc cagcagcacg
     1681 tccctttcgt tccgccgtat tcccatccgt tcaatcctct ggcttccttg agggcaacgt
     1741 tgctcgacat ggacgcgggc acgcgtcagc gtctcgtgac caggctgttc gacgcgacct
     1801 gggccgaaag ccgcgatctg ggttcgcccg aggtggtggc gcagatctgc gccgaggttg
     1861 gcgttcctga cgctcgcgag cgcattcagg acccggcgat caaggaacgt ttgttcgctg
     1921 cgagtcaacg ggcgatcgag ctcggcgtgt tcggtgtccc gacgatgatc gtagacggtg
     1981 agctcttctg gggtacggac agctttcccc atctggctcg ctatctcgcg ggccaggatc
     2041 ccatccggag cgaggatatt gcagcctggg ccgccatccc tgcgagcgcg caccgcaagt
     2101 agagcgttac tggctttgcc aggcgaccat gccgccgcgc atcgatgcga ttcgcttgaa
     2161 gccgagcttc tcgagctcga gcgcgacacg gcctgagcga cgacccgatc ggcagatcgt
     2221 gatcaccggc tcctccttgt cccaggattt cgatgcttcg tcgacggttc ccatcgggat
     2281 cacctcgaca ccctcgatct tgccatcgga ctcggcttcg tcgatctctc gcacgtcgac
     2341 catccgaatt acgcccctat tttcggcaac ccaggcaagc gtcacttcgg gagtgccatc
     2401 cggtgtgcgc tcgatggccg cccagccccg ctctggcagt ggatcaccag acatcgacga
     2461 cggaggcacg atgccacaat gcaggttcgc gggaaccgcc acgccaagct tcttgggcgc
     2521 cggcagattg aggtggctca tgatctcgac gaactccgcc tcgctcttcc catccccaac
     2581 gcgcggattg agacgctttt cttcacccac ggtggtgaat gtgaagccgc ggtagtcgtg
     2641 gcccggccag atgatcgtgt catcggggag ggtgaagatc ttgtcgtgga tggaacggta
     2701 gagccgctcc gcgctgcctt gctggaaatc ggttcggccg gtgccgcgaa tgaagagcgc
     2761 atctccggtg aaagcgtgct tgtggtcgga tgtgacgtag gtgacgcacc catcggtgtg
     2821 acctggcgtc gcgcgcacct cgagttcgag cgaaccgaac ttcagtttgc tcccgtcgtc
     2881 caccagtacg tcggcgcccg cggtcccggc gtttgcgccc acgaccgcct tactgcccag
     2941 acgctgacgc agaagcccgc aacccgtgat gtgatcggcg tgcgtatgag tgtccagggt
     3001 atacatcagc ttgaggccca gctccttcag tagcttgatg tcacgctcga gttggtcgat
     3061 gacggtgtcg atcagcaccg cctcgccgct tgcctcgtcg gcgagaaggt atgtgtaggt
     3121 ccaggtttca tgatcgaaga gttgccgaag cagcatggaa ggcctcctga gacttgcgag
     3181 aagcacgggg gttcccgtgg ctaagtcact gaattaacag cactcggggg gttctgcaaa
     3241 cccagtcgat acaaatcact ggcggtttgg gaccggtgct gctttgaatg tgcaccatgg
     3301 agtcttttac acccattgct tccacggtcg gtggtctaat gatcggcgtc gcggccgccg
     3361 ggctgctctt gtttcacggc cggatcgccg gtatcagcgg catcgccgga ggcatctttc
     3421 gccgggagtc cggtgacatt tcctggcgtg tcatgttcat cgttggactt ttcaccgctg
     3481 gcgcggtgtg gagccaattc tttcccctcg attatgaggt cgggatcgag cgttcgacgc
     3541 tggcgctaat cattgcgggt ctcacggtcg gcattggcac gcaactcggg agtggttgca
     3601 cgagcgggca cggggtctgt gggatcggac gcttgtcgaa gcgttcaacg gttgcgacga
     3661 tctcgttcat gatcaccgcc gcgatcaccg tattcgtcgt caacgaggtt ctcggaggct
     3721 cgatatgaag gtcaaaatcg gcgccttcct cggcggatcg ctcttcggtg ttggcttggc
     3781 catcgcgggg atgacgcaac cggccaagat catcggcttc ttcgatttct ttggcagctg
     3841 ggaccccagc ctggccttcg tgatgctcgg cgccatcgtc gtttacacgc cggtctatcg
     3901 atgggcgatt cgaacttggc aaaggccaat ctgggcgccg gcgttttccc tgccaacgcg
     3961 cgaagacatc gacgctcgac tcatcgtcgg gtcggccatc ttcggcgtgg gctggggcct
     4021 cgggggctac tgtccgggcc ctgcgctgac ttccgttggg gcgggttcgg agcaggcctt
     4081 cattttcggt gccgcgatgc taatcggagt cggggcctat cagcttttca tgcgagtccg
     4141 ggagcgccgg accgggggcg cagtggatcc gcaagccgcc gccatgatcg acggatgagc
     4201 ctgagcgttc tggaagatcg gtagcgtggg cgggtacacc gactggctga ggaaagtcga
     4261 ggaggcagcg gaccgcgccg aggtagagcg gtggcgcgca gagccgcgct cgtcggtata
     4321 cgacgccaag ctcttgcgcg agcagaccga ggagctcacg cgaacacgcg agacggggaa
     4381 agcgctagag ctcgttccat cgctgcgcga agccttgtac cggcagctcg gtgaactggc
     4441 ggatcctcgg ctctacgcgg tcactccact cggcaccaag taccgggtcg aggcttttta
     4501 cgaagaggca ctggcgtgca tcgcgtacct ggcagacccc gatcagaccg ggctatcccc
     4561 cgagttcacg ctcgctgggt ttcgaagggc ggcccacatc ttcgggcgtt cggcgctcct
     4621 gctctccggc ggtgcgacgc tcggcttctt tcatctggga gtcgtcaagg cgctttcgga
     4681 gcaagggctc ttgcccaacg tgctttcggg cgcctccatg ggcgcactca tcgcctgcgg
     4741 aaccggcgtt cgaaccgacg aagaaatcaa gacgctcttc gaggacccac gcgtcatccg
     4801 caccgatgcc ctcaagcgtc ttccgcccaa gcgcgccgcg aaagagcgcg ccgtctacga
     4861 tccagcacga ctcgcagagg tggttgcgaa caattgcggc gactacactt tcgaagaggc
     4921 acacgcgcgc acaggccgca ccgtgagcat cagcgtttca ccgactcgtg agcgccagaa
     4981 accaagagtt ctgtgccact acacgacgcc caacgtgttg atgaacagcg ctgcggtcgc
     5041 gtcggcgtcg attcctggag catttccagg tgccatgctt cagcagcgcg gtccttcggg
     5101 cgagcccatc ccctacatgt cgaccgaacg ctggatcgac ggctcgctca aaggcgacct
     5161 gccgatgcgc cggctgagtc gattgcacaa cgtcaaccac ttcatcgtga gtcaggtgaa
     5221 tccgcatgtc gccccggttc gcagcgtcac aggtaagcgc gggatcgtcc ccgtcgtttc
     5281 gggcgtgatc ctgtcgagct tccgcgcaca gatgacgcac caactcgagc tcgcccgcga
     5341 cttgaccaag agcacccccc tctacagcac gttcgacgtc gcccactcgc tggcaggcca
     5401 gccctacggc ggcgacatcg acatccatcc gaggctccgt cccgtcgcgc tgctccgggc
     5461 catgtcgaac atctcacacg aagagctcaa gaagcacatc cgagaaggcg aatacgcgac
     5521 ctggccgtta ctcgcgcgag ttcgggacca gacctgcgta tcccgggccc tcgacgccgc
     5581 gatcgatcgg ttgtcggccg aattggcgcg ctgagacggc gcccctccat gcactcccgg
     5641 tgtcagtgcc agtgccagtg tcagtgtcgg tgtcagtgcc ggtgtcagtg ccagtgccag
     5701 tgtcagtgtc ggtgtcggtg tcagtgccgg tgtcggtgtc ggtgtcggtg tcggtggtca
     5761 tccgtgggga tctgcatagg agggcggctc gggatgcgcg gcacagcgat tgggcgggag
     5821 gctagacgca gggaaacttg aagagacggc cgagcgggtc ccaatcgcga gcacgtgcgt
     5881 cgcgagccgc tctccgatcg ttacagctgg aagaatcact ctgtcagagc cagcgccagc
     5941 gccagtgtca gtgttcatcc gtggggatct gcataggagg gcggctcggg atgcg
//
LOCUS       DASRZF010000152         5205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3886271, whole genome shotgun sequence.
ACCESSION   DASRZF010000152 DASRZF010000000
VERSION     DASRZF010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3886271"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VFG22_06920"
     CDS             <1..291
                     /locus_tag="VFG22_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HET6416010.1"
                     /translation="LILDEPCACLDPGARELLIDDLDTLCAQKKGPTLILVSHHVEEI
                     PSCATHALLLKEGHTLAAGPLSEVLRNDTISELYEHTCQVGAANGRYWLRLR"
     gene            complement(350..763)
                     /locus_tag="VFG22_06925"
     CDS             complement(350..763)
                     /locus_tag="VFG22_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6416011.1"
                     /translation="MEINGIAHTFVTVGDFEKARAFYGELLPFLGLKNVMDIPGTYYC
                     VGGRTGFAIREASDENRGERFDQNRVGLHHICFRARSREDVDEAYEFVRNLGATIIHA
                     PEEAVWAPGYYSILFEDPDGIRLEVNYVPGKGLLE"
     gene            complement(774..1982)
                     /locus_tag="VFG22_06930"
     CDS             complement(774..1982)
                     /locus_tag="VFG22_06930"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6416012.1"
                     /translation="MIYLEVPKKLKPIVNQAHQVAKHVFRPISRKYDRAEHDYPKELD
                     LLAALMDGLSAGGGQSGAGVDGLRQGAGGDEGIRNGANMSTCLGLTELCWGDIGLALS
                     IPRQGLGNAAISAVADAEQKRRFAGTWAAMAITEPGAGSDSANIRTTARLDGDHWVLN
                     GEKIYVTAGSRADSVVVWATLDRNLGRAAIKSFVVPHGTPGMEVVRLDKKLGIRASDT
                     ASITLTDCRIPKENLLGTPDIDTKQGFGGVMQTFDNTRPVVAGMAVGVARAALEQTRE
                     LLSAEGIEVRYAGSIFKKTSAEAELIRMEADWEAGRLLMLKAAWMADNGIPNSTEASM
                     SKAKAARVANQITLRCVELCGGLGYSEDVLLEKWARDSKILDIFEGTQQIQLLIIARR
                     LLGKRSSELR"
     gene            complement(1992..3314)
                     /locus_tag="VFG22_06935"
     CDS             complement(1992..3314)
                     /locus_tag="VFG22_06935"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6416013.1"
                     /translation="MDNEKIIGDVMGGGLRVLNRIADSPLVERLGLEGRAQKLVYQGA
                     KRAAETAAKAAKRLAPKRSKERSRSSTKFDLSLSEEQQLMRDTLRRFADDVMQPAARE
                     ADDRSSAPDDLLEAFSELGAIAMSVPESLGGAAERVSAASQMLIVEDLARGDMGLAYA
                     VLSPLSFVNALVHWGDTEQQKRLLPSFVEGDLTAASIAMVEPHPLFDASTLRTTAKKQ
                     GSDYVLRGEKSMVALADRASWFLVSAEIPKLGPRFFLVERDAPGIEIVPEAAMGLRSA
                     SLGRVRLDKVHVPSEALLGGEAALKEGKKAHQDAVNRARVAWGAMSLGTAQALLDYAI
                     PYCNERVAFGEPISHRQAVAFMIADIGIELESMRMLVYRAAGRIDAGLDCQREAYLAR
                     TLCSEKGMEIGTNGIQLLGGAGFIKDHPMETWYRQLRSIGVMDGNLLV"
     gene            3417..4277
                     /locus_tag="VFG22_06940"
     CDS             3417..4277
                     /locus_tag="VFG22_06940"
                     /inference="COORDINATES: protein motif:HMM:NF024981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HET6416014.1"
                     /translation="MLSGLDKALIAALTLTLMVGMGASLTPADYRRALERPRAVLIGL
                     LSQFGWVPFLAYLAVRGLGLEGTDALGLMLMACTSGGNASNMFSYFSRADLALSISMT
                     AVSTLVSVVMMPVLLYVYAASFAQSEMQIPYFAVMGTLALMLAPIAAGMWIRRRSLDA
                     AKRVERVGGLAGALLLVVLLITSVSDQLDLLISARDESLVACVFVVVSAMVLGYGLAR
                     LAGLGPRQRRAVSLETGVQNAPLAIAVILATFPEADQDALLKLPFLYAMTALCVGTVA
                     TLAYRRFPSA"
     gene            4308..>5205
                     /locus_tag="VFG22_06945"
     CDS             4308..>5205
                     /locus_tag="VFG22_06945"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET6416015.1"
                     /translation="MGFRGILVCAILLSGCGESTKGGAGGNGGIAGSGGEGGTAGGLG
                     SAGSGGAGGIAGTGGAGGTGTVSHLEVTPYISGLDNPWDIAWLPNGTVLVTERTGPLN
                     VYVDGIDSAPFEIVPEDLVANGEGGMLGLEVDPSFATNGYVYVCMCSNAGGENDVRLL
                     RLTLSTPDGDELLARDDIVTGMPYSSGRHSGCRPRFGPDGYLWVGTGDAAIGTTPQDD
                     DSLGGKVLRIDRNGAAAPGNPGGRRWYSKGHRNVQGVAFRPSDSLGVSAEHGPSVDDE
                     INPLLPGNFGWDPVPGYNESVPM"
BASE COUNT          924 a         1675 c         1600 g         1006 t
ORIGIN      
        1 ctgattctcg acgaaccttg cgcctgcctc gaccccgggg cgcgagaact gctgatcgac
       61 gacctagaca ctctctgcgc ccagaagaag ggccccacgc tcatcctagt aagccaccac
      121 gtcgaagaga tcccgagctg cgccacccat gcgctcctgc tcaaagaagg ccatacgcta
      181 gcggcaggtc ccctgagcga ggtcctacga aacgatacca tcagcgagct ctacgaacac
      241 acgtgccaag tcggcgccgc caatgggcgc tactggctgc gcctccgcta gcggtggaag
      301 caaaccacgc tcgagctacg aaacggtgac tggccccgct cctcgcagcc tactcgagca
      361 agcccttccc cgggacgtag ttgacctcta gacggattcc atccgggtct tcgaagagga
      421 tggagtagta gccgggtgcc cagaccgcct cctcgggagc gtgtatgatc gtcgcgccga
      481 ggtttctcac gaactcgtag gcttcgtcga catcttcgcg cgagcgcgcg cgaaagcaga
      541 tgtggtgaag accgactcgg ttctggtcga aacgctctcc gcggttctcg tccgaggcct
      601 ctcggatggc aaagccggtg cggccgccca cgcagtagta cgttccaggg atgtccatga
      661 cgttcttcaa tcctagaaac ggcagaagct ctccatagaa ggcgcgcgcc ttttcgaagt
      721 cgcccaccgt cacgaacgtg tgcgcaattc cgttgatctc catcggcttc tccttaccgg
      781 agctcggacg accgttttcc gagcaagcgc cgagcgatga tgagcaattg aatctgctgg
      841 gtgccttcga atatgtcgag gatcttcgaa tcccgtgccc acttctcgag taggacgtct
      901 tcggagtaac cgaggccgcc gcaaagctcg acgcagcgga gcgtgatctg gttggccaca
      961 cgggccgcct tcgccttgga catcgatgcc tcggtggagt tcggaatgcc gttatcggcc
     1021 atccaggctg ctttcagcat cagaaggcgc cccgcctccc agtcggcctc catgcgaatg
     1081 agctctgcct ccgcggaagt ctttttgaag atcgagccgg cgtatcggac ctcgatgccc
     1141 tctgcgctga ggagctcgcg cgtctgctcg agggctgccc gcgcgacgcc aaccgccatc
     1201 cccgcaacga ccggccgggt gttgtcgaaa gtctgcatga ctcccccgaa cccctgtttg
     1261 gtgtcgatat caggggtccc gagcaggttt tccttgggta tcctgcaatc cgtgagcgtg
     1321 atgctcgccg tgtccgaagc tcgaatcccc agcttcttgt cgagacgcac gacttccatc
     1381 cctggcgtcc catggggcac gacgaacgac ttgatcgccg cgcgcccgag gttgcgatcg
     1441 agcgtggccc agaccaccac tgaatcggcc cggctccccg ccgtgacata gattttctcc
     1501 ccgttgagca cccagtggtc gccatcgaga cgagcggtcg tacggatgtt tgcggagtcg
     1561 gagcccgcgc cgggctcggt gattgccatc gctgcccagg tcccggcaaa acgccgcttc
     1621 tgttccgcat cggcgacagc actgatcgca gcgttaccga gcccttgacg gggaatggac
     1681 agagcaagac cgatgtcgcc ccagcagagc tcggtcagac caaggcatgt cgacatgttc
     1741 gcgccgttgc gaatgccttc gtcgccgcct gcgccttgtc gcagaccatc gacgcccgca
     1801 ccgctttgcc caccgcccgc gctcagaccg tccatgagcg cggcgagcaa gtccagctct
     1861 ttcgggtagt cgtgctcggc gcgatcgtac ttccgggaaa ttgggcggaa tacgtgcttg
     1921 gccacctgat gcgcctgatt gacgatcggc ttgagcttct tcggaacttc caaatagatc
     1981 atctgaatcc cctaaaccag caggttgccg tccatgacgc cgatggacct cagttgccga
     2041 taccaagttt ccatcgggtg gtccttgatg aaaccggctc caccgagaag ctgaatgccg
     2101 ttcgtaccga tctccatgcc cttctcggag caaagagtgc gagcgagata ggcctcgcgc
     2161 tggcagtcga ggccggcatc gatgcggcct gcggcgcggt agaccagcat gcgcatcgac
     2221 tcgagctcga ttccgatgtc ggcgatcatg aaggcgaccg cttgccggtg tgaaatcggc
     2281 tctccaaagg caacccgctc gttgcaatac gggatggcgt agtccagcaa ggcctgggcc
     2341 gtcccgagcg acatcgcgcc ccacgcgaca cgcgcgcggt ttactgcatc ctgatgagct
     2401 ttcttgcctt ccttgagcgc agcctcccct ccgagcaaag cttcggaagg cacgtgcact
     2461 ttgtcgagac ggacgcgacc gaggctcgcg gaccgaagac ccatcgcggc ctccgggacg
     2521 atttcgatgc cgggtgcatc gcgctccacc agaaagaaac gaggaccgag cttgggaatc
     2581 tcggccgaaa ccaggaacca gctcgcgcgg tccgcgaggg ccaccatcga cttttctccg
     2641 cggagcacgt agtccgagcc ctgcttcttc gctgtcgtgc gtagcgtgct tgcatcgaaa
     2701 agcggatgcg gctcgaccat ggcaatgctg gcagccgtca gatccccttc gacgaacgag
     2761 ggcaagagac gcttttgttg ttctgtgtcc ccccaatgaa ccagagcgtt gacgaaggac
     2821 agaggggaca gcaccgcata ggccaagccc atgtcgccgc gggcgagatc ctcgacgatc
     2881 agcatctggg aagcggccga aacccgttcg gccgccccgc cgagcgattc gggcaccgac
     2941 atcgcgatgg ctccgagctc actgaacgct tcgagcaggt cgtccggagc cgaggagcga
     3001 tcgtcggcct ctcgcgcggc cggctgcatc acgtcgtcgg cgaatcgccg aagtgtgtcc
     3061 cgcatgagct gctgttcttc gctcaagctg agatcgaact tcgtcgagct gcgactgcgc
     3121 tccttcgacc gcttgggcgc aagccgtttc gcagcttttg cggcggtctc tgcagcgcgt
     3181 ttagcccctt gatagacgag cttctgggcg cgaccttcga gcccgagccg ttcgacgagc
     3241 ggcgagtctg cgattcggtt gagcacgcga aggccgccgc ccatcacgtc gccgatgatc
     3301 ttctcattat ccacgaacag atgctgggcc cgagtcgaat agaggtcaat acggggacgg
     3361 cggtcttgga cgaaagattc cagttttgct taatcctgcg catgtatgat gctggaatgc
     3421 tcagcggtct cgacaaagcg ctcatcgcgg cgcttacgct gaccctgatg gtcggcatgg
     3481 gcgcttcgct cacgcccgct gactacaggc gcgccctcga acgtccacgc gccgtgttga
     3541 tcggactctt gtcgcagttc ggctgggttc ccttcctcgc atatctcgcg gtccgtggcc
     3601 tcggtctcga gggaaccgat gccctcgggc tcatgctcat ggcctgcacc tcgggaggca
     3661 acgcgagcaa catgttcagc tacttctccc gggcggacct ggcattgagc atctcgatga
     3721 cggccgtctc gacgctggtc tccgtggtga tgatgccggt ccttctttac gtatacgctg
     3781 cctcgttcgc gcagagcgag atgcagattc cgtactttgc cgtcatgggc acgctggcct
     3841 tgatgcttgc gccaatcgcg gcaggcatgt ggattcggcg acgctccctc gatgcggcaa
     3901 agagagtgga gcgtgtgggc gggctcgcag gggcgttgct gctcgtggtt ctcctgatca
     3961 cgagcgtgtc ggaccaactc gacctcttga tcagcgcgcg agacgaaagc ctggttgcgt
     4021 gcgttttcgt tgttgtgagc gccatggtgc tcggctacgg gctcgcgcgg ctcgccggtc
     4081 ttggcccccg tcaacgccgc gctgtctcgc tcgaaaccgg tgtccaaaac gcgccgctcg
     4141 caatcgcagt catcctcgcg acgtttcccg aagctgatca ggacgccctt ctgaaattgc
     4201 cgtttctcta cgcgatgacc gcactttgcg tgggcactgt cgctacattg gcataccgcc
     4261 gctttccctc ggcttgaact cagtcccgtc aaccgcagaa tgaagccgtg gggttcaggg
     4321 gtatcttggt ctgcgccatc ctgctcagcg ggtgtggcga atcgacaaaa ggcggagcgg
     4381 gcggcaacgg cgggatcgcg ggatctggcg gcgaaggtgg aaccgctggc gggctcggtt
     4441 ctgcgggcag cggcggcgcc ggtgggatcg cgggcaccgg gggtgccggc ggcacgggaa
     4501 cggtgtccca tctcgaggtg actccctaca tttccggcct cgacaacccc tgggacatcg
     4561 cctggctgcc gaacggaacg gtactggtga cggaaagaac aggcccgctc aacgtgtatg
     4621 tcgatggcat cgactcggcg cccttcgaaa tcgtcccgga agacctggtg gcgaacggcg
     4681 aaggcggcat gctcggcctc gaggtcgatc cgagctttgc gacgaacggc tatgtctacg
     4741 tttgcatgtg ctcgaacgct ggtggcgaaa acgatgtgcg cctacttcgt ctgacgctga
     4801 gcacgcccga cggcgacgag ctcctcgccc gcgacgacat cgtcaccggc atgccctaca
     4861 gctcgggaag gcacagcgga tgccggccgc gcttcggacc cgatggttac ctttgggtcg
     4921 gtaccggcga cgctgcgatt ggcacgacgc cgcaggacga tgactcgctc ggcggcaagg
     4981 tgcttcgcat tgaccgcaat ggcgccgcag cgcccggtaa tccgggcggg cgccgttggt
     5041 attcgaaagg gcaccgcaac gtgcaaggcg tcgcgttccg tccgagcgac tcgctcggcg
     5101 tttcggcgga gcacggcccc tccgtggacg atgagatcaa cccgctgcta cccggaaact
     5161 tcggctggga tcccgtgcct ggatacaacg aatcggttcc gatga
//
LOCUS       DASRZF010000153         3262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_792603, whole genome shotgun sequence.
ACCESSION   DASRZF010000153 DASRZF010000000
VERSION     DASRZF010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792603"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="VFG22_06950"
     CDS             <1..476
                     /locus_tag="VFG22_06950"
                     /inference="COORDINATES: protein motif:HMM:NF014418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HET6416016.1"
                     /translation="LRAEQEAALDRFRHWVDAHAEGAFIEYKPQAIAVHVRAIAETDP
                     ERAERLLREADERARELGLHVRRGKALREAEAERQDKGDALREIFERSGAKSVFFAGD
                     DITDFPAIDFAIQHGVGAFVRSEEQRGMPSEEAELLESVTEVAEILSRLLEKIRI"
     gene            515..1339
                     /locus_tag="VFG22_06955"
     CDS             515..1339
                     /locus_tag="VFG22_06955"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HET6416017.1"
                     /translation="MLDRDIAEAARIAREAGAILMAVYRTDFSVAYKTEADPVTEADT
                     RANAYIVAELEKAFPGDGVVAEETEDQSAALRGGRCWYVDPLDGTKEFVARNGEFSVM
                     LGLAIDGVSAAGVVYQPVNDKLYTGVVGEAAFLEREGERYKLEVSRVSEPSQLKLVVS
                     RSHRNRAVNAVVKRLGIRRETTSGSVGLKAGLIAEQQADVYLHISDKSSMWDACGPEA
                     VLKAAGGRFTDLNGQPYRYGGTDMRNRRGILACNAAAFDCVLPVARDAARAVGLID"
     gene            1349..1945
                     /locus_tag="VFG22_06960"
     CDS             1349..1945
                     /locus_tag="VFG22_06960"
                     /inference="COORDINATES: protein motif:HMM:NF017534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6416018.1"
                     /translation="MAPVDWKEAWKAGRTPWDAGASAPALLDLIAQGRVPAGRVLVPG
                     CGTGYDLATLARSDREVVGVDLSEDAKTAFLRAHPELPGDVVYEVTDFFSFAPEERFD
                     FVWDYTFFCALDPGQRGTWGETMRRLIKPGGLLATLLFPFEDPISDREGPPWSINTAL
                     VRNHIEDAFEELEAREVENTHAGREGKERLALWRRREP"
     gene            complement(1942..2508)
                     /locus_tag="VFG22_06965"
     CDS             complement(1942..2508)
                     /locus_tag="VFG22_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416019.1"
                     /translation="MAWSKCPVAVRAAAMLLVSLAGCPGRERSVEPPEPRRDGKSLRA
                     IDPYGNSPSRFGVVPLHAGFSPDPRVVGGRASGQVPAESMRRKCRGWISESPDYLLRA
                     DTAFFKLHILARSRGDLVLVVRKPNGEVLCNDNRSGTKDPMIASDIPIGTTQVWVGVH
                     EQGEELDYRLGFSEVKWQSSSISLPKND"
     gene            complement(2631..>3262)
                     /locus_tag="VFG22_06970"
     CDS             complement(2631..>3262)
                     /locus_tag="VFG22_06970"
                     /inference="COORDINATES: protein motif:HMM:NF025702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HET6416020.1"
                     /translation="AGDTNVDILVDGVTAIEDLEFAEATGFIELPAGNYDFGIAPAGS
                     TDSVFEFPATLVDGAVLTVVAYRTVASGDDAPVNVLVFDGSTAELPPGQGRVLVGHGA
                     DDTLLDPVDVIVPAACPPPLLNDFMFGAVEGPLDLDAGTVGIAFSLASDENECQVDVG
                     PLDAPVTADVVTLLIAVDNDTTDESVAPAVYALIDDFSGMGIPTLEASP"
BASE COUNT          635 a         1009 c         1055 g          563 t
ORIGIN      
        1 agctcagggc ggagcaagaa gccgcgctcg atcgatttcg gcactgggtc gacgcgcacg
       61 ccgagggcgc tttcatcgag tacaagcccc aagccatcgc cgtgcacgtt cgcgcgatcg
      121 ccgaaacgga ccctgagcgc gcagagcgcc tgcttcgtga agcggacgag cgcgcgcgcg
      181 agctcggtct gcacgttcgc cgaggcaaag ccttgcgcga agccgaagcg gaacgtcagg
      241 acaaaggcga cgctttgcgg gaaatcttcg aacgctcggg agcgaagtcc gtgttctttg
      301 caggtgacga catcaccgac tttcctgcga tcgatttcgc gatccagcac ggggtcggcg
      361 cattcgtccg ttcggaagag cagcgcggca tgccctccga ggaggccgag ctgctcgaaa
      421 gcgtgaccga agtcgccgaa atcttgtcgc gcttgctgga gaagatccgg atctgagcgc
      481 tggcaccggt atgccctcgt ggtagagatt tcagatgctg gatcgagaca tcgcggaggc
      541 cgcacgcatc gcgcgcgagg caggcgcaat cttgatggcg gtgtaccgaa cggactttag
      601 tgtggcctac aagaccgagg ccgacccggt aaccgaagcc gacacgcggg ccaatgcgta
      661 catcgtcgcc gagctcgaaa aagccttccc aggtgacggc gtcgtcgcgg aagagaccga
      721 agaccagtcg gccgcactac gggggggtcg ctgttggtac gtcgatccgc tcgacggtac
      781 caaagagttc gtagcaagaa acggcgagtt ttccgtgatg ctcggtctcg ccatcgatgg
      841 ggtttccgcg gccggcgtgg tctatcagcc ggtcaacgac aagctttaca caggcgtcgt
      901 gggcgaggcc gcttttctcg agcgcgaggg cgagcgatac aagctcgagg tcagccgcgt
      961 ctccgagcct agccagctga agctcgtggt ctccaggtct cacagaaacc gcgccgtgaa
     1021 tgcggtcgtc aagcgactcg gcattcgtcg tgaaactacg agcggctcgg tcggattgaa
     1081 ggcgggcctc atcgcagagc agcaggccga cgtctatctt cacatctcgg acaagtcgag
     1141 catgtgggac gcgtgtggtc cggaggcggt tctcaaggcc gccggtgggc ggttcaccga
     1201 tctgaacggc cagccatacc ggtacggcgg gaccgacatg aggaatcgca gaggcattct
     1261 cgcctgcaac gcagcggcct tcgactgtgt gctccccgtg gcaagagatg ccgctcgagc
     1321 ggtcgggctc atcgactgag gaggacggat ggcgccggta gattggaaag aagcctggaa
     1381 agcaggacgc acaccctggg atgctggcgc ctcggcgccc gccctcctcg acttgattgc
     1441 gcagggtcgg gtcccggcgg ggcgcgtcct cgttccgggc tgtggcaccg gctacgactt
     1501 ggcgactcta gcgcgctcgg atcgcgaggt cgtgggcgtc gatctgtccg aggacgcgaa
     1561 aaccgctttt ctaagagccc atcccgagct gcccggagac gtcgtttacg aagtgacgga
     1621 cttcttttct ttcgcgcccg aggagcgctt cgatttcgtc tgggactaca ctttcttctg
     1681 cgctctcgat cccggccaac gcggcacctg gggcgagacg atgcggcgcc tgatcaagcc
     1741 aggcgggctt ctggcgacgc tgctctttcc gttcgaagat ccgatctccg accgagaggg
     1801 tccgccctgg tcgatcaaca cggccctggt acggaaccac atcgaggacg ctttcgaaga
     1861 gctcgaagct cgcgaagtcg agaatacaca cgcgggccgt gaaggaaaag agcggctggc
     1921 gctctggcgc cgccgcgagc cctaatcatt cttgggcagc gagatcgacg aggactgcca
     1981 ctttacttcg gagaatccga gccgatagtc cagctcttcc ccctgctcgt ggacgcccac
     2041 ccaaacctgc gtcgtgccga tggggatgtc ggaggcgatc atcgggtcct tcgttccgct
     2101 gcgattgtcg ttgcatagca cctcaccgtt cggctttcgc acgaccaaga cgaggtcgcc
     2161 tcgcgagcgc gcgaggatgt gcaacttgaa gaacgccgta tcggcccgga gcaggtagtc
     2221 cggggactcg gatatccagc ccctgcactt gcggcgcatc gattcggcgg gaacctggcc
     2281 gctggccctg cccccaacga cccgggggtc ggggctgaat cccgcgtgca gcggaaccac
     2341 gccgaaacga gaaggggaat ttccatacgg atcgatcgct ctcaacgact tgccgtcgcg
     2401 cctgggctcg ggcggctcca cggatcgttc tctaccgggg catccggcga gggataccag
     2461 caacatcgca gccgcgcgca cggccacggg acacttgctc caagccatca ccgtgccgat
     2521 cgtcgtagcc ggactccgag cgacccgtca actgtacaat agagtggggc cgttcaccca
     2581 aagtgagcgg ccccaagtct acgctacaaa aggggatgaa tgaggaagga ctagggactc
     2641 gcttctagag taggaatgcc catccctgag aaatcgtcga taagcgcata aaccgccgga
     2701 gcgaccgact cgtcggtcgt gtcgttgtcg accgcgatga gcagcgtcac aacgtctgcg
     2761 gtcaccggcg catcgagcgg gccgacatcg acttggcact cgttctcgtc actcgccaga
     2821 gagaaggcga tccctacggt accggcatcg aggtcaagag gtccttcgac cgccccgaac
     2881 atgaagtcgt tcaagagtgg tggtggacaa gccgcgggca caatcacgtc gaccggatcg
     2941 agaagcgtgt cgtcggcgcc gtgcccgacc aggacgcgac cctgccccgg gggaagctca
     3001 gcggtgctgc catcgaaaac caatacgttg accggcgcgt catccccgct cgcgacggtt
     3061 cgataagcga cgaccgtgag aaccgccccg tccacgagcg ttgccgggaa ctcgaagact
     3121 gaatcggtcg agcctgccgg tgcgatgccg aagtcgtaat ttccagctgg aagctcgatg
     3181 aagcccgtgg cctcggcgaa ctcgaggtct tcaatcgcgg tcacaccgtc cacgagaatg
     3241 tcgacattgg tgtcgcctgc gg
//
LOCUS       DASRZF010000154         3549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_103712, whole genome shotgun sequence.
ACCESSION   DASRZF010000154 DASRZF010000000
VERSION     DASRZF010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103712"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1112
                     /locus_tag="VFG22_06975"
     CDS             <1..1112
                     /locus_tag="VFG22_06975"
                     /inference="COORDINATES: protein motif:HMM:NF028442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HET6416021.1"
                     /translation="ADVPESESLYAGGSYDFLRLSASAAHVAGKRYVSSETFPSVGAL
                     SLSEDQARLLMGRAFSAGINRLVHHGNAYPYLHQDGQRWYPFHPLPDSAFATGPLDIT
                     FDIHPGAEIWASLPALNAGAARLSYALSRGSAVAEVAWLHPDWRVKNFPSFGVEPGAN
                     ESQISASLRRAGFSYDRVSRGALASSTSAEGILTVGETSFEALLIEDVHAADPEMLAA
                     IEQAVAAGVPVVWMGELPSRAIGLADAEIRDADVNARIESLRGAVTFVGSAEQIPAAI
                     SSAGVTPPLSPVDAAGMQISVQHRRVINGDLYFLFNESFAARTEQIRIAEAFGDARLL
                     DPETGAAITTDLQDDVLTLSLDGARGVILWVARAE"
     gene            1195..3165
                     /locus_tag="VFG22_06980"
     CDS             1195..3165
                     /locus_tag="VFG22_06980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HET6416022.1"
                     /translation="MYFYRTFFLGLGLLICLAGAAACGEGTADGGGRTGGAGGDGGTG
                     GVGAVGGVGGDAGIGGHAGSGGVDPTDDCGDGERQPTEACDDGNRRSDDGCAGDCMMI
                     EEGFRCPSPGATCVPLVCGDSRIDPPEQCDDNNDKGSDGCSATCELEPGWVCLQPGVA
                     CGAAECGDGIVAGFEQCDDGNAVTNGCSASCQLEDGFKCETPGAPCTATACGDGVREG
                     TEQCDDGNLTPFDGCDLNCKNEPDCSGGDCQSVCGDGVILPGTDEACDDGNTVSGDGC
                     SAICEIENGFTCTTVASELPDELVVPVIFRDFRSQQTGAGVDPTFNPDFNPPGTGTHL
                     GITTDQLDAEGKPQFNSGGAGVGSGGWPQSTTSFAAWYRTSPTLVADGNIEVVRELTL
                     TRPNPSIPNRYEFRSSSFFPLDDDGWELQAPPLQELPMTNGHNFGFTTEVHYFFVYQG
                     DEVLRFEGDDDLWVFVDGRLCMDIGGIHAAISGVMSFDPGVVDGNAARQAIVDACIAD
                     LGLQAGKVYEVAIFHAERHVTQSNFRLTLDGFVTERSECSHTCGDRIRTRYEFCDDGS
                     AENTGEYGHCNGSCTALGPHCGDGILQEAFEDCDNGENLGGLNGCNPDCSSGPFCGDG
                     IRQPGLGESCDAGQQNGQPGSGCSALCEVVVQ"
     gene            complement(3210..>3549)
                     /locus_tag="VFG22_06985"
     CDS             complement(3210..>3549)
                     /locus_tag="VFG22_06985"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET6416023.1"
                     /translation="VEIESLVNGEVRQHQSTSDLIYTPVEMLHFVAAAFPNASLSKGT
                     IVLSGTPGGVAMSTPRWLVRLSNLIGVSRFRKLSAKLEGDSSRFLSVGDEVMVRGPGL
                     GQVAVTISAE"
BASE COUNT          674 a         1073 c         1165 g          637 t
ORIGIN      
        1 tggcggacgt cccggaatcc gagagtctgt acgccggtgg ctcctacgac ttccttcgat
       61 tgtcggcatc cgctgcgcac gtggcaggca agcgctacgt cagcagcgag acgttcccct
      121 cggtcggcgc tctgagcctg agcgaagacc aagcgcgtct tttgatggga cgagctttta
      181 gtgcgggcat caatcgactg gtacaccacg ggaacgcgta cccctacctc caccaggatg
      241 ggcagcgctg gtatccgttc caccctctcc ccgacagcgc gttcgccacc ggtccgctcg
      301 acatcacgtt tgacatccat cccggcgccg aaatttgggc atcgctgccg gcgctgaacg
      361 ctggggccgc ccgactgagc tacgccctga gccgtggcag cgcagtcgcc gaggtggcct
      421 ggctccatcc ggactggcgc gtaaagaact tcccgagctt cggtgtggaa ccaggcgcga
      481 atgaatctca gatcagcgca tccttgcgcc gcgccggatt ctcctacgat cgcgtaagtc
      541 gaggagcgct ggcctcctcg acgagtgccg aggggatctt gaccgtaggt gagacctctt
      601 tcgaagctct gctcatcgaa gatgttcatg ccgccgatcc ggaaatgctc gcagcgatcg
      661 agcaggcagt ggcagcaggt gttccggtgg tttggatggg ggagcttccg tcgcgtgcga
      721 tcggtctcgc ggacgcggaa atcagagatg ctgacgtgaa tgctcgcatc gagagcctgc
      781 gaggcgcggt cacattcgtg ggctcggctg agcagatccc cgcagccatc tccagcgcgg
      841 gagtcacacc tccgttgagc ccagtcgacg cggccggaat gcagatctcc gtacaacatc
      901 ggcgcgtcat caatggtgac ctctacttcc tcttcaacga gagcttcgcg gcgcggaccg
      961 aacagatccg aatcgctgag gcattcggcg acgcgcggct tctcgacccg gaaaccgggg
     1021 cagcgatcac cacggacctt caggatgatg tcttgacgct gagcttggat ggtgcccgtg
     1081 gggtcattct gtgggttgcc agggccgagt gaacgcgggt aggacccaac aaagcgccca
     1141 aaaacgcgct ttccgggact ggcgggtctc ggcttcggca tctatactgt aaaaatgtac
     1201 ttttacagga ctttcttcct tggactggga cttctcatct gcctggctgg cgcagcagcc
     1261 tgcggtgagg ggaccgcgga cggcgggggc cgaaccggag gcgctggagg ggacggcggc
     1321 accggcggtg tcggagctgt gggcggcgtc gggggcgatg cgggcatcgg aggtcatgcg
     1381 ggaagcggcg gcgtcgatcc gaccgacgac tgcggggacg gcgagcgaca gccgacggag
     1441 gcctgcgatg atgggaaccg ccgaagcgat gatggctgcg ctggcgactg catgatgatc
     1501 gaggagggtt ttcgctgccc gagtccaggc gcgacttgcg tgccactggt ctgcggggac
     1561 tctcgtatcg acccgccgga gcagtgcgac gacaacaatg acaagggctc ggacggttgc
     1621 agcgcgactt gtgagctcga gcctggctgg gtgtgcttgc agccgggcgt tgcctgcggt
     1681 gcagcggaat gcggagatgg catcgtcgcg ggcttcgagc aatgtgacga cgggaacgcg
     1741 gtgaccaacg gttgctccgc gagctgccag ctcgaagacg gattcaaatg cgaaacgccc
     1801 ggcgcgcctt gcacagccac ggcctgcggc gacggcgtac gcgaaggaac cgaacagtgt
     1861 gacgatggga acctgacgcc tttcgacggc tgtgacctca actgcaagaa cgaaccggat
     1921 tgctccggcg gtgactgcca atccgtctgt ggcgacggcg tgatccttcc agggacagac
     1981 gaagcctgcg acgacggcaa cacggtgagc ggtgatggat gctccgcgat ttgcgagatc
     2041 gagaacggct tcacctgcac aaccgtggct tccgagctcc cggacgagct cgtggtgccg
     2101 gtgatcttcc gagacttccg aagccagcaa acgggagccg gtgtcgatcc cactttcaat
     2161 cctgatttca accctcctgg gacggggacc caccttggga ttacgactga ccagctcgac
     2221 gcggagggca aaccccagtt caactcgggc ggtgccgggg tcggtagcgg cggctggccg
     2281 cagagcacca cgtccttcgc ggcatggtat cggacgagcc ccacgctcgt cgcggatgga
     2341 aacatcgaag tggttcgcga gctcacgctc acacgaccca atccgagcat tccgaatcgc
     2401 tacgagttca ggagcagctc gttcttcccg ctcgacgacg acggctggga gctgcaggcg
     2461 cctccgctcc aagagcttcc aatgaccaac ggacacaact tcggattcac cacggaggtg
     2521 cactatttct tcgtgtacca gggcgacgaa gtgcttcgct tcgagggaga cgacgacctc
     2581 tgggtattcg tggacggccg actctgcatg gacatcggcg gaattcacgc tgcgatcagc
     2641 ggtgtgatga gtttcgaccc tggggtggtc gatggcaacg ccgcacgaca ggcaatcgtc
     2701 gacgcctgta ttgccgatct aggcctgcaa gcaggcaagg tatatgaggt cgcgatcttt
     2761 cacgccgagc gtcatgtgac acaatcgaac ttcaggctga cgctcgatgg cttcgtcacg
     2821 gagcgttccg agtgcagcca tacgtgcgga gataggattc gtacccgtta cgagttctgc
     2881 gatgacggaa gcgcagagaa tactggcgag tacgggcact gcaacggttc ctgcacggcc
     2941 ctcggcccgc actgcggcga tggcatcctc caagaggcct tcgaagactg cgacaacgga
     3001 gaaaacctcg gcggtctgaa cggctgcaat cccgactgca gcagcggacc gttttgcggc
     3061 gacggcatcc gtcaacccgg gctcggcgag tcctgcgacg ccggccaaca gaatggtcag
     3121 ccgggatctg gctgcagcgc tctttgcgaa gtagtggtcc aatagccgaa ccgcagcatc
     3181 ggcggagctc gagcgctcta gcggagctgt cactcggctg agatcgtcac ggcgacttgc
     3241 ccgaggcctg ggcccctgac catgacttca tctccgacac tgaggaagcg cgagctgtct
     3301 ccttcgagct tggccgagag cttgcgaaaa cggctcacgc cgatgaggtt gctcaagcgt
     3361 acgagccagc gaggcgtgct catcgcgacc ccgccgggcg tcccgctgag cacgatcgtg
     3421 cccttcgaga gtgacgcgtt ggggaaagcg gcagcaacga aatggagcat ctcgaccggt
     3481 gtgtagatca gatccgacgt ggactgatgc tgacgcactt cgccgttgac caggctctcg
     3541 atctccaca
//
LOCUS       DASRZF010000155         2346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_104256, whole genome shotgun sequence.
ACCESSION   DASRZF010000155 DASRZF010000000
VERSION     DASRZF010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104256"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VFG22_06990"
     CDS             complement(<1..585)
                     /locus_tag="VFG22_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HET6416024.1"
                     /translation="MRTSFTSDRPPESRLERWAYALKPASWPKLFVPALFGQALGASE
                     KGALGLEALGWGFAFTLFGVGFIVLLNDWGDREVDAIKRNMFPDGCSPKTIPDRLIDS
                     RAIAIAGVVFGAATLLVSAGAERFLSRPFAFEAGLACMLLFVAYTLPPIRLNYRGGGE
                     LLEMAGVGLALPLYNAYLQAGSVALTAWPWILGFS"
     gene            complement(592..1656)
                     /locus_tag="VFG22_06995"
     CDS             complement(592..1656)
                     /locus_tag="VFG22_06995"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HET6416025.1"
                     /translation="MTLLFVYVGLALSVSFICSILEAALLSITPSYMAKLEQDRPKVG
                     ARIRSLKLDIDRPLAAILSLNTIAHTVGAAGVGAEAQRLWGSEVLAIASVVLTLLILL
                     LSEIIPKTLGAIYWRRMTGFIAGFLPPLITLLLPLVWLSEVITGFLRRRKVAQTISRE
                     EFAALARVGEEQGIFDASEGRILRNLFLFGSLRARDIMTPRTVMFSLEENTTVREALA
                     ERGSMMFSRIPLWKQTPDQVTGYILKDQLLLRAARNELDLPVSAFAREALMVPDTSPL
                     PALFEKLLDYREHIAVVVDEYGGVDGVVSMEDVIETLIGLEIVDEVDSVHDMRAMARA
                     KWEVRAKHIESDPPTPPEPD"
     gene            complement(1653..>2346)
                     /locus_tag="VFG22_07000"
     CDS             complement(1653..>2346)
                     /locus_tag="VFG22_07000"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6416026.1"
                     /translation="RLMKRLSFQFHWRNEGYESFDDFVAEFRSSMRKKLRKERRIAAE
                     ANLRIEELSGDAIAVEDWHTLERLYRATCRRKGSYPYLTPEFFELAPSTLGGSPLVLA
                     AKDQGQIVAASINFAKGGHLYGRYWGASQRHDMLHFELCYYRLIEHAIAQGMRRFEAG
                     AQGTHKLRRGLMPVPIHSAHWVRHPILQQAVADFLPREAFSVQQQIRELAEHGPFRRL
                     ADEDSARPRASR"
BASE COUNT          515 a          701 c          721 g          409 t
ORIGIN      
        1 cgagaacccc aagatccaag gccatgcggt cagcgctaca gacccggctt gcagataggc
       61 gttgtagagc ggaagtgcga gaccgacacc agccatctcg agaagctctc cacctcctcg
      121 gtagttcaaa cggatcggcg ggagcgtgta agcaacgaac agcagcatgc aggccaatcc
      181 ggcctcgaac gcgaagggtc gcgatagaaa gcgctccgcg ccggccgaga ccagcaaggt
      241 cgcggcccca aagacgacgc ccgcgatcgc gatcgcgcgc gagtcgatca ggcggtcggg
      301 tatcgtcttc ggcgaacagc catccgggaa catgttccgc ttgatcgcgt cgacttcgcg
      361 atcgccccaa tcgttcaaca atacgatgaa gcctacaccg aagagcgtga acgcgaaccc
      421 ccatcccaaa gcctcgagcc ccagcgctcc tttctcgctt gcgccaagcg cctgcccaaa
      481 gagagcgggc acgaatagct taggccaact ggctggcttc aaagcatacg cccatcgctc
      541 taggcgcgac tccggaggcc ggtcgctggt aaacgaagtc ctcatgggtc ctcaatcagg
      601 ttccggcgga gtgggcggat cgctctcgat gtgcttggca cggacctccc acttcgcgcg
      661 cgccatggct cgcatgtcgt ggacagagtc cacctcgtcc acgatttcga ggccgatgag
      721 cgtttcgatc acatcctcca tgctgacgac tccatcgacc ccaccatatt cgtcgacgac
      781 cacggcgatg tgctcgcgat agtcgaggag tttctcgaac agagcgggaa gcggactcgt
      841 gtccggcacc atgagtgcct ctcgagcgaa agcactcacc ggaagatcga gctcgttgcg
      901 cgctgcccga agcaagagtt ggtccttcag tatatagcca gtcacttgat ccggggtttg
      961 cttccaaagt ggaatccgag aaaacatcat ggagccgcgc tcggcaagtg cttccctgac
     1021 ggtggtattc tcttcgagcg agaacatgac ggtccggggc gtcatgatgt ctcgtgcccg
     1081 aagtgatcca aagagaaaca aattgcgaag aattctgccc tctgatgcgt cgaagatgcc
     1141 ttgctcttcg ccgacgcgag cgagcgcggc gaactcctcg cgggagatgg tctgggcgac
     1201 ctttcgacgc cgcaggaaac ccgtgatcac ctcggaaagc cagaccaagg gaagaagcaa
     1261 cgtaatcaac gggggcaaga atccggcgat gaagccggtc atgcggcgcc aatagatcgc
     1321 tccaagcgtc ttgggaatga tctccgaaag caagaggatg agcagcgtca ggaccaccga
     1381 agcgatggct aggacctcgg aaccccaaag acgttgcgcc tctgcgccca cgccggctgc
     1441 accaacggtg tgggcaatcg tattgagact gaggattgcg gcaaggggtc gatcgatgtc
     1501 gagcttgagt gagcgaatcc gtgcgccaac cttgggacga tcctgctcga gcttggccat
     1561 gtaggaagga gtgatgctga ggagcgcagc ctctaggatc gagcagatga acgagacgct
     1621 gagtgccaag ccgacataga cgaacagcag ggtcatcggg aagcccgggg tcgcgcgctg
     1681 tcctcgtcgg cgagacgccg aaatgggccg tgttccgcga gctcgcggat ctgctgttgc
     1741 acggagaacg cctctcgagg gaggaaatcc gcaaccgctt gctggagaat cggatgccta
     1801 acccagtgtg cgctatgaat gggcaccggc atcaagcctc gccgcagctt gtgggtgccc
     1861 tgagcgcccg cctcgaagcg acgcatgccc tgcgcgatcg cgtgctcgat caggcgatag
     1921 taacagagct cgaaatgaag catgtcgtga cgctgcgacg caccccaata tcggccgtag
     1981 agatgtccgc ccttcgcgaa attgatcgat gccgcgacga tctgaccttg gtccttcgcc
     2041 gcgagaacga ggggcgaacc gccgagcgtg ctcggcgcga gctcgaagaa ctcgggtgtg
     2101 aggtaaggat acgaaccctt gcggcggcag gtcgcacggt agaggcgctc gagcgtgtgc
     2161 caatcctcga ccgcgatcgc gtcgccactg agctcctcga tacggagatt ggcttcggcg
     2221 gcgatgcgac gctctttccg cagcttcttg cgcatggacg agcggaattc ggcgacgaaa
     2281 tcgtcgaacg attcgtatcc ttcgtttctc cagtgaaact gaaacgacaa gcgtttcatg
     2341 agccgg
//
LOCUS       DASRZF010000156         2428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2513145, whole genome shotgun sequence.
ACCESSION   DASRZF010000156 DASRZF010000000
VERSION     DASRZF010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2513145"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="VFG22_07005"
     CDS             complement(<1..896)
                     /locus_tag="VFG22_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416027.1"
                     /translation="MCGYRQPPLAALCSLAIGSLLLASHPALAQPPSAESPTEPAEEA
                     EGVEPARPDAVPTDPQQAFIPDIVFARMAENPVSGITRVPVANFANFGIPPNKRVGDA
                     LVLAPIIPVRLKGGWSLLTRTTIPALVTLPFGRQASGAPDPNGRTTGFGDIGLEVLGA
                     KQIRGKKGQFYDVGVGPFVGAPSATDDFLGRGRWLLGPEIALGISAKKWVTVLIARNE
                     WSVGKSSSRADVNQMWIQYFLFYNLPKLFYLVYEPIVTVNWERQSGDRATLPVGIGWG
                     RHLRLPKRPRLAMTTRYSGFYNA"
     gene            967..1665
                     /locus_tag="VFG22_07010"
     CDS             967..1665
                     /locus_tag="VFG22_07010"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET6416028.1"
                     /translation="MPTRTLEHGIPGILHGLSDELRTALEEAEVRVRYRDGEQIQARG
                     DRTPGLRIVRAGAVRAGNVGAGGEYLVLGVFGPGQTFGEMTLFAGLPRTFDVHASGPT
                     TIGYIDEARMDRLLRDHPELARLFLRTLATQLHAALEFVEDLRRLSVTVHVAKLLSTM
                     VKTETKIASLEVTQESLGKILGVTRISVARALDRLEEDGLLRRCYGRIEVPDVACLRA
                     WVAEHSKLPPARSA"
     gene            1910..>2428
                     /locus_tag="VFG22_07015"
     CDS             1910..>2428
                     /locus_tag="VFG22_07015"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET6416029.1"
                     /translation="MAKKPPDIHLFTASTMNGWKPLIFLQEAGVDYDLTHVDFGKREQ
                     KSAEYRRLNSNGRIPTILDRGNDDFVVFESNAILWYLAEKFERFLPEGPKGRSQVLQW
                     LMFQVSGVGPMMGQAMFFQRIAAPQGHCDAYAIERCVGESRRLLEVVDAQLAGKSYLL
                     GDVYTIADMAMYP"
BASE COUNT          448 a          738 c          786 g          456 t
ORIGIN      
        1 gcattgtaga agccgctgta tctcgtggtc atcgcgaggc gcggacgctt cggcagtcga
       61 aggtgccgtc cccagccgat cccaacaggc agggtcgcgc ggtcacccga ctgcctttcc
      121 cagttgaccg tgacgatcgg ctcgtagacc aagtagaaga gcttcggtag attgtagaag
      181 agaaagtatt ggatccacat ctggttcacg tcggcccgac tgctgctctt tccaaccgac
      241 cactcgttgc gcgcgatgag caccgtcacc cacttcttcg cgctgatgcc cagggcgatc
      301 tccgggccaa gcagccagcg tcctcgtccg agaaaatcat cggttgcgga tggagctccg
      361 acgaacggtc cgacaccgac gtcgtagaac tgccccttct tgcccctgat ctgcttggcg
      421 ccaagcacct ccaatccgat gtccccgaag cccgtcgttc gcccgtttgg gtcaggtgcg
      481 cccgaggcct ggcgcccaaa gggcagcgtc accagggcgg ggatcgtggt ccgcgtcagg
      541 agactccagc cccccttgag ccgaaccggg atgatcgggg cgagcacgag ggcgtccccc
      601 acgcgtttgt tcggcgggat gccgaagttt gcgaagttgg cgacggggac ccgtgtgatc
      661 cccgagaccg ggttctcggc cattcgtgcg aacacgatgt cggggatgaa ggcttgctga
      721 gggtcggtgg gcactgcgtc aggccgggcc ggctcgacac cctccgcttc ctccgctggc
      781 tcggtgggac tttcggccga aggcggctgg gcgagggccg ggtggctcgc gagcaacaac
      841 gacccgatcg cgagggagca caatgctgcc aagggcggct gtcgataccc acacatgcga
      901 cttccaaaca acctcggctt gctagcatgg ttgagtctgg gaaattgtcg acgaggctac
      961 aatcacatgc caacccggac cttggaacac ggaatccctg ggattctcca tgggctcagc
     1021 gacgagcttc gaaccgccct cgaggaggcc gaagtacgcg tccgctatcg agacggcgaa
     1081 cagattcagg cccggggcga cagaacgccg ggactgcgga ttgtgcgtgc aggtgccgtt
     1141 cgagccggga acgtcggggc agggggggaa tacctcgtgc tgggcgtctt cgggcccggt
     1201 cagacctttg gcgaaatgac gctgtttgcc ggcttgccgc gaacgttcga tgtccacgcc
     1261 tcgggcccga ccacgattgg gtacatcgac gaggctcgca tggaccgact cctgcgagat
     1321 catcctgagc tcgccagact gttcctgagg accttggcga cccagctcca cgccgcgctc
     1381 gagttcgtcg aagacctcag gaggctttca gtgaccgttc acgtcgcgaa gctactgtcc
     1441 acgatggtca agacggagac aaagatcgcg agtctcgaag tgacccagga atccttgggc
     1501 aaaatcctcg gggtgacacg catctccgtc gctcgcgcat tggatcgcct cgaagaagac
     1561 gggttgctcc gtcgatgcta cggaagaatc gaggtccccg acgtggcctg cttacgggcg
     1621 tgggtcgccg agcattcgaa gcttccgccg gcgaggtctg cataggtatc caagccgttc
     1681 gctggccggc aggacgtcct tgcgatcgcg cacgaacgcc gtgcggggga tgccgatgtc
     1741 gtgacgactg aggatctgca gcgagcgcag cttgtcgcgc gagttcgcag attcggattt
     1801 ccgtggttga gctcgatggc gaagcgaagc gtgttctcag gcttggtgcg ggtgcaaagc
     1861 cgacggggcg gcgtcgctga cttgcggaag cgggctacac tggctctcca tggcgaagaa
     1921 accgcctgac attcacctgt tcacggcatc gacgatgaac ggttggaagc cgctgatctt
     1981 tctccaggag gctggcgtcg actacgacct cacgcacgtc gactttggca aacgcgaaca
     2041 gaagtcggcg gagtatcggc gtctcaactc caacggccgc atcccgacga tcctcgatcg
     2101 tggcaacgac gatttcgtgg tcttcgagtc gaacgccatc ctctggtacc tcgccgagaa
     2161 gttcgaacgg ttcctccccg aggggcccaa aggccgttcc caggtactcc agtggctgat
     2221 gtttcaagtc agcggcgtag ggccgatgat gggccaggcg atgttcttcc aacgcatcgc
     2281 tgcgccccag ggtcattgcg acgcgtacgc gattgagaga tgcgttggcg agtcacgtcg
     2341 cttgctggag gtcgtcgacg ctcagctggc gggcaagagc tacctgctgg gtgatgtcta
     2401 cacgattgcc gacatggcga tgtatccg
//
LOCUS       DASRZF010000157        16223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3891510, whole genome shotgun sequence.
ACCESSION   DASRZF010000157 DASRZF010000000
VERSION     DASRZF010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 16223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16223
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3891510"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VFG22_07020"
     CDS             <1..252
                     /locus_tag="VFG22_07020"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET6416030.1"
                     /translation="VDIRDAASFGRYRIPSAIHIPANELASRTAELQPFDGRIRVLYG
                     RSNEAQEWSQKLQADGVQVGFLTGGFLHWEADGLEVERG"
     gene            249..593
                     /locus_tag="VFG22_07025"
     CDS             249..593
                     /locus_tag="VFG22_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416031.1"
                     /translation="MKRKLQLVGDSGGQPLAMPRFVNLGQLTVSVIDAGDGSETQRFV
                     DTLIETDAALTDPKADAAADFSIVIRATPRTSEGRLRERVLEEGADLVLGSARPVFAK
                     LFGSACARWVNS"
     gene            630..1778
                     /locus_tag="VFG22_07030"
     CDS             630..1778
                     /locus_tag="VFG22_07030"
                     /inference="COORDINATES: protein motif:HMM:NF010639.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HET6416032.1"
                     /translation="MAEVREYIPGLAGIPAARSSICLIDGSIGKLQYRGYPIEVLAER
                     GTYEEVAYMLLFGRLPTRPELDEFREQLIAERGLKFRIIDLLKTLPESGEPMDALTAA
                     VAAMGMFYKGDHISDPEFLRLSMIRLVAKLPTIVAAWDRIRRGDDPIKPSTELSHAAN
                     FLYMLEGKVPTRLAERVFDVALILHAEHSMNASTFTARVTGSTLTDPYAVVASAIGSL
                     AGPLHGGANEQVLSTLNSIEDRSPHAIRRWAEDRLARKEKVMGFGHRVYKVKDPRATI
                     LQGLATELFKKYGSTPDYDLAVELETQMADLVGGKGIYPNVDFYSGIVYQKLGIPTDL
                     FTPIFAISRVAGWLAHLLEQLEGNRIFRPTQIWVGEADRTFVPIEDRG"
     gene            complement(1972..3123)
                     /locus_tag="VFG22_07035"
     CDS             complement(1972..3123)
                     /locus_tag="VFG22_07035"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HET6416033.1"
                     /translation="MRESASRVDADDVHDESGAVSTAPSRACAAIALLAFALGCQSDR
                     EPGAPIAPGSVPDTTGRPLLSRTRPLMSTVFRIQVDAPPANAETVIRQAFEEVERLET
                     VLSEWRDESDISKINALAGKKSVKVGDDTLRVVDAGMDVSAWSRGAFDLSWAALWGLY
                     DFRPDSIRIPTAEEVRPRLPLIDYKNIRVSKKYKTVFLKKRGMAIGTGGIAKGYALDH
                     AGAILRGGGVDNYMIFGGGQVQVHGKRNGRPWRVGIQHPRDPDKYFAALESTGASFST
                     SGDYEHAAFDGAGKRWHHIIDTKTGLPADKSLSVTIMTSEGIYADALSTAAFVLGPQK
                     AIKMLSRIAYPAEAVIVGADCRLYMTPKAEQRLRLNVELVDGRLPNCTP"
     gene            3143..3910
                     /locus_tag="VFG22_07040"
     CDS             3143..3910
                     /locus_tag="VFG22_07040"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HET6416034.1"
                     /translation="MRAMFGVHWGRLLLVLTCFSVSPPERASAFGDATAVEIVSLDVG
                     NGLEGGRGVAERLAWEVRKRSSVEPILEPGRARLDKTSLFRSPFIYWRGSEEFPPLSE
                     EAIEGLRKYIHRGGFILIDDATAGAEEFDRAVRRELLRAFPALPLKPISEDHTIYRSF
                     YLLQRPVGRVMGPPFLEGITYGDRIAIVYSRHDLGGAFQRDKLGAWSQAVVPGGEGQR
                     ERAIRLGVNLVLYALCTDYKDDQVHAPFIMRRRGSGR"
     gene            3920..6130
                     /locus_tag="VFG22_07045"
     CDS             3920..6130
                     /locus_tag="VFG22_07045"
                     /inference="COORDINATES: protein motif:HMM:NF018757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HET6416035.1"
                     /translation="MADWQWQLGILGGSAAALVLGLALFAAWLVELRSIRGESDRRRR
                     WALGALGGLGILAVYLLALQITLVRETFEDVAGGTGILLDSSRSMTLSGENGARSDAV
                     RALIKQWQEDDRVSPTVYLFGSHPRAAIWTDLEQSYNPADDETKIREALNYVLGKGAA
                     QELGALIIITDGADPSFRVPGLALGPEAPAIHAVVVGADNPLDDQAIVALHADTTAYV
                     GVPALIRADVRAVGELRGSELRVQLWHESRLEQETVALLDETGSGTVSFEVTPNRTGR
                     ALYRLLIPAAASDQVPENNDRAALLRVGRERLRALLVAGRPTWDVRFLRDFLKRDGSV
                     DLVSFFILRAAADLTAAPTNELALIPFPTDELFREHLGSFDVVIFQNFDYAPYEMQTY
                     LPRIRDYVQRGGSFVMIGGDRSFSEGRYAGTAIEEILPVKLGGAGVLTGSFRAEVNKS
                     LARHPIVALGPDPSLTRKTWQTLPPLHGMNMVTGIKDGAQVLLHHPRARLNGRARLPV
                     LVVGEAGSGRVAAMTTDASWRWRFAADDATVGSDEYELFWDRMIRWLTRDPLLEPARI
                     STDREHYGAGAEISVSGRFRDSGYRPMKKARVALHVQPDSGEGPPVIADADEDGVLQA
                     ILRAPTEQGAYEVVASVDGKDLAREVFIVERSGDELANLEAIPDRLQALAEKTGGRLF
                     LGTEGVPPLSELASSSRRATGVVSKQPLLTPWFILLTILILGTTWVLRRRWGRR"
     gene            6205..11520
                     /gene="uvrA"
                     /locus_tag="VFG22_07050"
     CDS             6205..11520
                     /gene="uvrA"
                     /locus_tag="VFG22_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET6416036.1"
                     /translation="MQPTKLRGARTHNLRSIDLDIAPGTYLAIVGPSGAGKSSLAFRT
                     LYAEGQRRYVESFSAYARQFLERLSRPPVDSLDPVPVGIAVDRQAPVRTSRSTVGTMT
                     EIADYAKHLWAHAAELRCPGCGQQVHRDAPTRAAQSVMSALPGEKLLVTYPVAVADEE
                     HFIGVREGLLRDGYRRVRVKDEVRDLDAVRPSDVVNGRSTHLDVLADRTVARAEDQTR
                     LVEALEVAMRRGGGRASVWSAAGAELGFSEGLHCPSCDRSFRNATPGLFSFNSPIGAC
                     ETCRGFGRTIEIDLDRVIPDYDISPDEGAIRAWQGKATTWERRELKKSAKKAGIPLDK
                     PLRDWSPAELAWLIDGDDVGYPKGWWGIRSWFKWMESRAYKMHVRVFLSRYRKYETCR
                     DCQGTRLKAEALAWHIDGLSLPELYGLPASNALSFLEKQEQRFIGDAGASLLWREALG
                     RLRALHDVGLGYLSLDRTSRTLSGGETQRVALTSALGATLNGAMFVLDEPTVGLHPRD
                     VERLLGVVRALSKDDNIALVVEHDPEMILGADRVIELGPGAGENGGQIVFDGTPSALR
                     KAKTATGAALKASRKTSRPRREAKAWIVLKGASGHNLRSVDVEFPLGVMTCVTGVSGS
                     GKSSLVLETLLPAAQRQLGLKSDGAPLAYESVSGFESLRGVVGVDQAPLGRTSRGNAA
                     TYLKIWEVFRKRFTQQPLAKERGYKPGFFSFNVAGGRCEACRGDGAETVEMQFLADVV
                     FSCPECQGRRFVGPALDVELKGLCIADVLELTAHQAAEHFADDALIARALAPLIDVGL
                     GYLRLGQSLNTLSGGEAQRLKLAEALLRATPGSLLIFDEPTAGLHANDVEPLLEVLDG
                     LVDRGDTVVVVEHDMHVAAHADYVIDLGPGAGGAGGKLVAHGSPEAVAKKRSSQTATY
                     LARALGMSKRAKPRGSRKIGKASSKPLQEIRIAGAREHNLKNVTVHIPREQLVVLTGP
                     SGSGKSTLAFDVLFAEGQRRYLETLSPYARQYMPQLPRPAVDQVLGVPPSVSLEQRLT
                     RAGANSTVATVTEVSHYLRLIWARIGVLHCVDCEVPISARPASQLAHDLGRRFAARKL
                     TLLAPVVRGRKGIHRELLKKAMKDGFTEARIDGKVQPLEPGMKLDRYTEHDVELVVGR
                     VEASSPELLPILEKALKVGDGAAWAIAGTEEMQLSSKRACPNCGRGYPELDPRFFSFN
                     TRQGQCPACEGKGEIVQKTGRGRRRKKSVERRLCDQCDQTRLSPLARGVSVDEMSITA
                     LFDQSVAEAIETLRGIDLEGREATIAKTPLQEALRRLSFLEEVGLGYLGLGRAANTLS
                     GGEMQRVRLAAQLGSGLTGVLYVLDEPTIGLHPRDTGRLLGALRGLVERGNSVLVVEH
                     DADTIRAADHVIDVGPGGGKHGGRIVAQGTLRAMKGSVTAAALERPPTIPAKRRPTAN
                     CSWLELGGVTHHNLRGVDVRFPLERLVAVTGVSGSGKSSLVREVLLRATREAVGMVNE
                     IAAGQHDRIRGFETLKRAIEIDQSPIGRTPRSVPATYIGIWNTVRQFLAGTPEARARG
                     YTASRFSFNVEEGRCPECKGQGAIHVEMAFLPDVDVPCESCNGMRFTPETLSVRWQGL
                     NAGEILELEVAEAVEVFSPVRTIRQPLQLLDELGLGYLHLGQASNTLSGGEAQRIKLV
                     SELAAGLNTGPTLYVMDEPTTGLHRDDVNRLLGVLNRLVDRGDTVAVIEHHPDVMLAA
                     DWIVDLGPEGGIHGGRVVAQGTPETIAKRARSHTGQVIRREMKRA"
     gene            complement(11527..12003)
                     /locus_tag="VFG22_07055"
     CDS             complement(11527..12003)
                     /locus_tag="VFG22_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416037.1"
                     /translation="MPSPEHAADLERLLSRAAEECDAREGIWFGNGSPQVLRLALEAH
                     GTGTGTEPAEIAFVEVRAVTTRGRAATDAVPSDLSCPVVGLSLSTLDELSSLFRTEVE
                     GGVQLSRLICPVAVFDFGPNGVRVREVRHGLTAADLQQRLATPLYCGPDLKELGSH"
     gene            complement(11978..13090)
                     /gene="carA"
                     /locus_tag="VFG22_07060"
     CDS             complement(11978..13090)
                     /gene="carA"
                     /locus_tag="VFG22_07060"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003280874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HET6416038.1"
                     /translation="MTEVAHLVLADGTRFSGTRFGAEGATTGEAVFTTGMTGYEEVLT
                     DPSYCGQIVTMTAPQIGNTGINLEDGEAKRPALRGFVVRELSAVESNWRTKESLDAYL
                     RRQGIVGISGVDTRTLTRHIRDHGAQLAALGTEDPATLHDRAKAATPMTGLDLTGEVT
                     TAKTYSWTEGSGVWSQQREGGRPWHVVAMDFGVKLNILRCLVDEGCKVTVVPATATAE
                     EVLALDPDGVFLSNGPGDPAAVTAGIGTVRALLGQRPIFGICLGHQLMSLALGGESYK
                     LKFGHRGLNQPVKDLKTGRVEVTTQNHGFCVDVDSLKNKCEVTHLHLNDGTCEGIEHL
                     ETGAFSVQYHPEAAAGPHDSRYLFRRFTDAIARARS"
     gene            complement(13093..14391)
                     /locus_tag="VFG22_07065"
     CDS             complement(13093..14391)
                     /locus_tag="VFG22_07065"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HET6416039.1"
                     /translation="MLNQLIVLRGGRVLDPACGLDTIADVVIEAGRIKSVGRDAGAAY
                     EAGDDVRIVDASGRWVCPGFVDLHVHLREPGQEYKEDIGSGLDAAAAGGFTAVCPMPN
                     TKPANDTRAITEMMLARAQQHGGTRLLPFGAVTKGQRGSELTEMADLRDAGAIGVSDD
                     GRCVMNAAVMRHAMEYARTFDMLVSQHCEDHDLTEGAQMHEGARSTQLGLRGWPRAAE
                     DIIVARDLILAETTGARYHVAHISSFGAVRLIREAKSRGLRVSAEVTPHHLLFTDEAL
                     LGYDTYCKVNPPLRELEDRESLREALADGTIDCIATDHAPHTDLEKDCEFEAASVGIN
                     GLESAIGSMFQLVRDGVLKPLRFVEVLSTAPARLIPDFEGGSLREGRPADVTLLDPET
                     QWILDKEALRSKSHNTPMLDCELTGRPIMTIVAGKVVHEL"
     gene            complement(14435..14677)
                     /locus_tag="VFG22_07070"
     CDS             complement(14435..14677)
                     /locus_tag="VFG22_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416040.1"
                     /translation="MRISNSFSEGEIQVLEFILQTLLRGGTPSMATRNKDFASVYRKV
                     MTMKKRVEDKKTHEKLSSSVGTVAAADDDDLQRKAG"
     gene            complement(14893..>16223)
                     /gene="pilB"
                     /locus_tag="VFG22_07075"
     CDS             complement(14893..>16223)
                     /gene="pilB"
                     /locus_tag="VFG22_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HET6416041.1"
                     /translation="VSSESAILSALEKAYAQPEISYEDIMEGFDESEIEVATSDDDEN
                     LLDLERASEDAPVIRLCNAILLNAIKQGASDIHVEPYERVLRVRYRVDGVLHEEMTPP
                     IKLKNAIASRLKIMSSLDIAERRLPQDGRIKLKLGKGREMDYRVSSVPTLWGEKIVLR
                     LLDKSNLQLDMTKLGFEQKPLSDFRDAIHQPYGMVLVTGPTGSGKTTTLYSAMSDLNE
                     VGTNISTAEDPIEFNLMGINQVQMHEDIGLNFAAALRSFLRQDPDIIMVGEIRDFETA
                     EIAVKAALTGHLVLSTLHTNDAPSTVTRLLNMGVEPFLVTASVNLVLAQRLARKICEE
                     CKVQVPTDEQVLIDVGFKDGEIADAVAFRGTGCRVCNDTGYKGRVALYEVMPFRDRLR
                     EIVLQGGSTAEVKDEMIRDGVATLRRAGLNKVLQGVTTIDEIVRVTAADN"
BASE COUNT         2951 a         5093 c         5148 g         3031 t
ORIGIN      
        1 gtcgacattc gcgacgccgc atcgtttgga cgctatcgaa ttccgtctgc gattcacatt
       61 cctgcaaacg agcttgcgag ccggaccgcg gagctgcagc cgttcgacgg gcggattcgc
      121 gttctctatg gccgcagcaa cgaggcgcag gagtggagcc agaagctcca agcggacggc
      181 gtccaagtcg gattcctgac tgggggcttc ctacattggg aggcggacgg actcgaggtc
      241 gagcgcggtt gaagcgcaag cttcagctcg taggcgattc gggcgggcag ccgctcgcca
      301 tgccgcggtt cgtgaacctg ggtcaactca cggtctcggt gatcgacgct ggagacggct
      361 cggagaccca gaggtttgtg gacaccctca tcgaaaccga cgcggccctg accgacccca
      421 aggccgacgc ggcggcagac ttcagcatcg tcatccgcgc aacgcccagg acctccgaag
      481 gtcgactacg ggagcgggtg ctcgaggaag gtgcagacct ggttctcggc tcggcacgtc
      541 ctgtatttgc aaagcttttc ggatctgctt gcgcccgttg ggtgaacagc taggttgccg
      601 cccgaccttt tagcccgaaa gtggagacga tggcggaagt gcgcgaatac attccaggct
      661 tggccggcat tccagcggcc cgcagcagca tctgtttgat cgatggatcc atcgggaagc
      721 ttcagtaccg agggtacccg atcgaggtgc tcgccgaacg tgggacctac gaagaggtcg
      781 cgtatatgct gctctttggg cgccttccga cccgccccga gctcgacgag ttcagggagc
      841 agctgatcgc cgagcgcggg ctcaagttcc gcatcatcga cctcttgaag acgctgccgg
      901 agagcggcga accgatggat gcgctgaccg ccgccgtcgc cgcgatgggc atgttctaca
      961 agggcgacca catctccgac cccgaattcc ttcgtttgag catgatccgc ttggtcgcga
     1021 agctgccgac catcgtcgcg gcctgggatc ggattcggcg cggagatgac cccatcaagc
     1081 cgagcaccga gctcagccat gccgcgaact tcctctacat gctggagggt aaggtcccaa
     1141 cccggcttgc cgaacgggtt ttcgacgtcg ctctgatcct ccatgccgag cactcgatga
     1201 acgcgtcgac attcacggcc cgcgtgacgg gatccacgct caccgacccg tatgcggtcg
     1261 tggcatctgc gataggcagc cttgcggggc cgctccatgg aggcgccaac gagcaagtgc
     1321 tttccacctt gaacagcatc gaggaccgct cgccgcacgc gatccgccgc tgggccgagg
     1381 accggctcgc gcgcaaggaa aaggtgatgg gcttcggcca ccgcgtctac aaggtgaaag
     1441 atccacgtgc gaccatcctg caagggctcg caacggagct tttcaagaag tacgggagta
     1501 cgccggacta cgacctcgcc gtcgagctcg aaacgcagat ggccgacctc gtgggcggga
     1561 agggcatcta ccccaacgtc gacttctaca gcggcatcgt ttaccagaag ctcggaattc
     1621 cgacggacct cttcacccca atctttgcga tttctcgggt ggctggctgg ctggcgcact
     1681 tgctcgagca gctcgagggc aaccggatct tccgcccaac gcagatttgg gtcggcgaag
     1741 ctgaccggac cttcgtgccg atcgaagacc gcggttagtc tccactcgag cctctggggt
     1801 cctacagcat gaacaattag gtagagatcc cgcgatgaag gcgcgggaag catctcacca
     1861 agatcgcagt ccttcgcatc gttgatgcga ccaatcagtc accatattcg cgtcaacttc
     1921 ggctccagcg accagggcac gggcgcgcgc ctgcaaggcg ggcgtcagca tctacggtgt
     1981 gcaatttggc aagcgtccgt ctaccagctc gacattgagg cgtaatcgtt gttcggcttt
     2041 gggtgtcatg tagagcctgc agtcggcgcc cacgatcact gcctcggcgg ggtaggcgat
     2101 tcggctcagc attttgatcg ctttttgggg gcccagtacg aacgctgcgg tgctcagggc
     2161 atccgcgtaa atgccttccg atgtcatgat cgtaaccgag agggatttgt ccgcggggag
     2221 gcccgtcttg gtatcgatga tgtggtgcca gcgcttgccc gcgccgtcga aggcagcgtg
     2281 ctcgtagtca ccggaggttg aaaacgacgc tcccgtggac tcgagcgcag cgaagtactt
     2341 gtctgggtcg cgcggatgtt ggatgccgac acgccaggga cgcccgttcc gcttgccgtg
     2401 cacctgaacc tggccgccgc cgaagatcat atagttgtcg acgcccccgc cgcggagaat
     2461 cgcgccggcg tgatcgagcg cgtacccctt ggcgattccg ccggtcccaa tcgccatgcc
     2521 gcgcttcttc aggaagacgg ttttgtactt cttgctgacc cgtatgttct tgtagtcgat
     2581 cagcgggagg cggggcctga cctcttcggc tgtgggaatc cggatcgaat cgggacgaaa
     2641 atcgtagaga ccccagagcg cggcccagga gagatcaaac gcacccctcg accatgctga
     2701 aacatccatc cccgcatcca cgacgcggag ggtgtcgtcg cccaccttga ccgacttctt
     2761 tcccgcgagc gcgttgatct tcgagatgtc gctctcatcg cgccattccg aaagcacggt
     2821 ctccaatcgc tccacttcct cgaaggcttg tcgaatcacg gtctccgcat tggcaggcgg
     2881 agcgtcgact tgaatgcgaa agaccgtgct catcagcggc cgcgttcgcg acaagagagg
     2941 gcggcccgtc gtatcgggga cgctgccagg tgcgatgggg gcgcctggtt cgcgatcgct
     3001 ctgacagccc aatgcgaatg cgagcaatgc aatcgcggcg caggcgcgag atggagccgt
     3061 cgacacggcg ccactttcgt cgtgaacatc gtctgcgtca acccggcttg cgctttcacg
     3121 catcgttggt actcctctct tcatgcgggc gatgtttggg gtgcattggg gtcgccttct
     3181 attggtactg acctgttttt cagtatcgcc tcccgagcgg gcatctgcgt ttggcgatgc
     3241 cactgcagtg gaaatcgtgt ccctcgacgt cggcaacgga ctcgagggtg gacgcggagt
     3301 ggccgagcgc ctcgcctggg aggtgcgcaa gcgttcgagc gtggagccga tcctcgaacc
     3361 cggccgtgcc aggctcgaca agacgtcgct gtttcgcagc ccattcatct actggcgtgg
     3421 cagcgaagag tttccaccgt tgagcgaaga ggccatcgaa ggactgcgca aatacatcca
     3481 tcgcggcggc ttcattttga tcgacgatgc aaccgccggt gcagaggaat tcgatcgcgc
     3541 ggttcgccgc gagcttcttc gcgcttttcc ggccttgcct ctcaaaccca tttccgagga
     3601 ccacaccatc tatcgctcgt tctaccttct acaacggccg gttgggcgcg tgatgggacc
     3661 gccgttcctc gaaggcatca cgtacggaga ccgcattgcg atcgtctact cgcgccacga
     3721 tctcggaggt gctttccagc gggacaagct cggcgcctgg agccaggccg tcgtccccgg
     3781 gggggaaggt cagcgcgaac gagccatccg cctaggcgtc aacttggtcc tctacgcgct
     3841 ttgtaccgac tacaaggacg accaggtcca cgcgcccttc atcatgcgtc gacggggctc
     3901 gggccgctga ggtttgcgcg tggcggactg gcagtggcag ctcgggattc tcggaggcag
     3961 cgctgccgcg ttggtgctcg gactggcctt gtttgccgct tggctggtcg agctgcgttc
     4021 gattcgtggc gaaagcgatc ggcggcgacg ttgggcgctc ggcgctctcg gcggtttggg
     4081 aatcctagcg gtctaccttc tcgctcttca gatcacattg gtgcgcgaga cgttcgaaga
     4141 cgtggccggt ggaaccggta ttctgctcga tagctcgcgg agcatgacgc tctcgggcga
     4201 gaatggcgcg cggagtgatg ccgtccgggc actcatcaaa cagtggcaag aagacgaccg
     4261 agtcagtccc actgtttacc tgtttggctc ccacccacgc gccgcgatct ggactgacct
     4321 cgaacaaagc tacaatccgg ccgacgacga aaccaagatc cgagaagccc tgaactacgt
     4381 tctcgggaag ggcgcggccc aggagctcgg ggccctcatc atcatcaccg atggcgccga
     4441 tccgagcttt cgggtcccgg ggctcgcgct gggtcccgag gcgccggcaa tccacgcggt
     4501 cgtggttggg gcagacaatc ctctcgatga tcaggcgatt gtcgctttgc acgcggacac
     4561 cactgcatac gttggagttc ccgcgctgat tcgcgccgac gtgcgcgcgg tcggagagct
     4621 acgcgggagc gagcttcgcg ttcaactctg gcacgagagt cgtctcgaac aggaaacggt
     4681 cgcgctgctc gacgagaccg gcagcggcac cgtgtctttc gaagtgacgc cgaaccgcac
     4741 ggggcgggca ctctatcgct tgctgattcc ggccgcggcg agcgaccagg tgccggaaaa
     4801 caacgatcgg gcggcgctcc tgcgggtagg gcgagaacgg cttcgcgcgc tcctcgtggc
     4861 aggtcgaccg acttgggacg ttcgattcct tcgggacttc ctcaagcgcg atggctcggt
     4921 cgacctggtc tcgttcttca ttctgcgcgc cgcggccgac ctcaccgcgg cacccacgaa
     4981 cgagctcgcc ttgattccgt tcccaaccga cgagctgttt cgcgagcacc tcggcagctt
     5041 cgacgtggtg atcttccaga acttcgacta cgcgccctac gagatgcaaa cctacctgcc
     5101 gcgcatacgc gactacgtcc agcgaggcgg ttcgttcgtg atgatcggtg gcgatcgatc
     5161 tttctcggag ggccgatatg ccggtactgc catcgaagaa atccttcccg tgaagctcgg
     5221 cggggccggc gtgctcacgg gcagcttccg cgccgaggtg aacaagtcgc tcgcacgcca
     5281 ccccatcgtc gccctgggcc ccgacccctc tctgacgagg aagacctggc aaacacttcc
     5341 accgctgcac gggatgaaca tggtcactgg gatcaaggac ggcgcgcagg tgctcttgca
     5401 tcatccgcgc gcccggctca acggccgcgc tcggcttccg gttttggtcg ttggcgaagc
     5461 cgggagcgga cgagtcgctg caatgaccac ggatgcatcg tggcgatggc gcttcgcggc
     5521 cgacgacgcg acggtgggct ccgacgagta cgagttgttc tgggatcgga tgattcgctg
     5581 gctcacccgc gacccattgc tagagccggc acgcatctcg accgatcggg agcactacgg
     5641 agccggcgcc gagatctcgg tgagtggcag attccgcgat agcggctacc gaccgatgaa
     5701 gaaggcgcgc gtggcgctgc acgtccagcc tgattcaggc gaggggcctc ctgtcatcgc
     5761 cgatgccgat gaagacggcg tgctccaagc gattctccgc gcacccacgg agcagggtgc
     5821 gtacgaagtg gttgcatccg tcgacggcaa ggaccttgcg cgtgaggtgt tcatcgtaga
     5881 acggagcggg gacgagttgg cgaacctgga ggccattccc gatcgactgc aagcccttgc
     5941 cgagaagacc ggagggcgtc tgttcctcgg caccgaaggc gtcccgccgc tgtccgagct
     6001 cgcgtccagc tcacgaaggg cgactggcgt cgtttcgaag cagcctctgc tcacgccctg
     6061 gttcattctg ctgaccatac tgatcctcgg cacgacctgg gtgctgcgca ggcgctgggg
     6121 acgtcgataa acgtcgagtg cgcgtgacca cgtgttccgg gctggacatc gctcgctccc
     6181 atcctagaga tagcagtcac gcccatgcag cccaccaagt tacgcggtgc gcgcacgcac
     6241 aaccttcgtt cgatcgatct cgatatcgcc ccgggcacgt acctcgcgat cgtaggtcct
     6301 tctggcgctg gtaagtcgtc actcgccttc cgaaccctct acgcggaagg tcaacggaga
     6361 tacgtcgaga gctttagtgc gtacgcgcgg cagtttctcg aacggctttc gagaccaccg
     6421 gtagactcgc tcgatccagt gcctgtcggg attgccgtgg atcgtcaggc gccggtgcga
     6481 accagtcgct cgaccgtcgg cacgatgact gaaatcgccg actacgcgaa gcacctttgg
     6541 gcccatgcgg ccgagctacg atgtcccggt tgtggccagc aggtgcatcg cgacgcaccc
     6601 actcgcgccg cgcaatcggt gatgagcgcg cttcccggtg agaagctgct cgtcacctat
     6661 ccggtcgctg tggccgacga agagcacttc atcggtgttc gcgaaggcct gctgagggac
     6721 ggctaccgac gggttcgggt caaagacgag gttcgcgacc tcgacgcggt tcgaccgagc
     6781 gatgtcgtca acggccgaag cacgcacctc gatgtcctcg ccgaccggac cgtggcacgc
     6841 gcagaggatc agacacgctt ggtcgaggcg ctcgaggtcg ccatgcgcag gggaggaggc
     6901 agggccagcg tctggtcggc ggcaggcgcc gagctggggt tctcggaggg gcttcattgc
     6961 ccgagctgtg accgttcgtt tcgcaacgca acgccgggac tgttcagctt caacagcccc
     7021 atcggagcgt gcgagacgtg ccgcggcttc ggacggacga tcgaaatcga tttggatcga
     7081 gtgatcccgg actacgacat ctcacccgac gagggcgcca tccgcgcctg gcaagggaaa
     7141 gcaaccacct gggagcgccg cgagctgaag aagagcgcga agaaggctgg gatcccgctc
     7201 gataagccgc tgcgagattg gtccccggcc gagctcgcct ggctcatcga cggtgacgac
     7261 gtcggctacc cgaagggctg gtgggggatt cggagttggt tcaagtggat ggagtcacgc
     7321 gcgtacaaga tgcacgttcg cgtcttcctc tcgcgctacc gaaagtatga aacctgtcgt
     7381 gactgtcagg gcactcgact caaggccgaa gccctggcct ggcacatcga cggcctgagc
     7441 ctacccgagc tctacggact cccagcgtcc aatgcgctct cgttcctcga aaagcaggag
     7501 caacggttca tcggcgacgc cggcgcctcc ctcctctggc gtgaagcgct cggtcgattg
     7561 cgggcgctcc acgacgtggg gctcggatat ctttccttgg atcgcacgtc gaggacgctt
     7621 tccgggggcg agactcagcg ggttgcgctg accagcgcat tgggggcgac gctcaacgga
     7681 gcgatgttcg tcttggacga acccaccgtg ggcttgcacc cccgcgacgt ggagcggctt
     7741 ctcggcgtcg ttcgggccct gtcgaaagac gacaacatcg ccctggtggt ggagcacgac
     7801 ccggagatga tcctcggcgc cgaccgcgtg atcgagctcg ggccgggcgc cggtgagaac
     7861 ggaggccaaa tcgtcttcga cgggacaccg agcgcgctga gaaaggcaaa gaccgcgacc
     7921 ggcgcagccc tgaaagccag ccggaaaacg tcgagaccgc ggcgtgaggc caaggcttgg
     7981 atcgtgctca agggcgcttc cggtcacaac ctccgctcgg ttgatgttga gtttccgctc
     8041 ggcgtgatga cctgcgtcac gggcgtcagc ggatcgggga aaagcagcct ggtcctcgaa
     8101 accttgctgc cagccgcgca gcggcagctc ggcctcaagt cggacggggc gccgctcgca
     8161 tacgagtcgg tctcgggctt cgaatccctt cggggtgtgg tcggtgtcga tcaagctcca
     8221 ttaggccgaa cttctcgcgg aaacgctgcg acatatttga agatttggga agtatttcga
     8281 aaacgattca cgcagcagcc actggcaaaa gaacgtggct acaaacccgg attcttctcg
     8341 ttcaacgtgg cgggcggtcg ctgcgaggct tgccgcggag atggggcgga gacggtcgag
     8401 atgcagttct tggccgacgt cgtgttcagc tgtccggagt gtcagggaag acgctttgtg
     8461 ggcccggcgc tggacgtcga gctcaagggc ctctgcatcg cagacgtcct cgagctgacc
     8521 gcgcatcagg ctgccgaaca cttcgccgac gacgctctga tcgctcgggc ccttgcaccg
     8581 ctgatcgacg tgggactcgg ctacctgcgc ctcggccaat cgctcaatac cctctccggt
     8641 ggggaagcgc agcgattgaa gctggctgag gccctccttc gggccacgcc aggctcactc
     8701 ctgatcttcg acgagccgac ggccggattg cacgccaatg acgtggaacc gctgctcgag
     8761 gtgctcgacg gtctcgtcga ccggggcgac acggtggtcg tggtcgagca cgatatgcac
     8821 gtcgccgcgc atgcggacta cgtgatcgac ctcggacccg gagccggtgg cgcgggcgga
     8881 aagctcgtcg cccatgggag ccccgaagcc gtagccaaga agcggtcgtc gcagacagcc
     8941 acgtacctgg ctcgagcgct cggaatgtcg aagagggcca aacccagggg cagccggaag
     9001 atcggcaagg ccagctccaa accgcttcag gagatccgaa tcgccggtgc gcgcgagcac
     9061 aacctcaaga acgtcaccgt tcacattccg cgcgagcagc tcgtcgttct cacgggaccg
     9121 agcggaagcg gaaagagcac tttggcgttc gacgtcttgt ttgccgaagg tcagcgacgg
     9181 tacttggaga cgctctcgcc gtacgcccgc cagtacatgc cgcagctgcc gagaccggcg
     9241 gtcgatcagg tgcttggcgt gccccctagc gtctcactcg agcaacggct tacgcgcgca
     9301 ggggcgaact ccaccgtggc caccgtcacc gaggtctcgc actatctgcg cttgatttgg
     9361 gcacggatcg gtgtgctcca ttgtgtggat tgcgaggttc cgatctccgc acgaccggca
     9421 tcgcaactgg cccatgacct cggccggcgc ttcgctgccc gcaagctgac cttgcttgcg
     9481 cccgtggtgc gcggtcgaaa aggcattcat cgcgagctgc tcaagaaagc gatgaaagac
     9541 ggattcaccg aggcccgaat cgacggcaag gtgcagccgc tcgaaccagg gatgaaactc
     9601 gaccggtaca ccgaacacga cgtcgagctc gtcgtgggcc gcgtcgaagc atcgagcccc
     9661 gagctgctgc ccattctcga gaaggcgttg aaggtgggtg atggcgctgc gtgggcgatc
     9721 gcgggcaccg aggaaatgca gctttcgagc aagcgcgcct gcccgaattg cggtcgcggc
     9781 tacccagagc tcgatccgcg atttttttcc ttcaacacac ggcaagggca gtgccccgct
     9841 tgcgaaggaa agggcgagat cgtccagaag acggggcgtg ggcggcggcg caagaaatcc
     9901 gttgagcggc gcctctgcga tcaatgcgat cagacccgac tctcccccct ggcgcgtggc
     9961 gtttccgtcg atgagatgtc gatcaccgct cttttcgatc agagcgtcgc tgaggcgatc
    10021 gaaactcttc gaggtatcga tctcgagggt cgcgaggcaa ccattgcaaa gacgcctcta
    10081 caagaagcgc ttcgccggct ctcctttctc gaagaggtcg gtctcggcta tctcggcctc
    10141 ggccgcgcgg ccaataccct gagcggaggc gagatgcagc gcgtgcgact cgcggcccag
    10201 ctcggaagcg ggctcaccgg agttctgtac gtgctcgacg agcccaccat cgggctacac
    10261 ccgcgggaca ccggccgtct gctaggcgct ttgcgcgggc tcgtcgaacg gggcaactcc
    10321 gtgctcgtcg tcgagcacga tgcggatacg attcgcgccg cagatcacgt gatcgacgta
    10381 ggaccgggcg gtggcaagca cggcggcaga atcgtggccc aaggcacact gcgcgcgatg
    10441 aagggatcgg tgacagccgc ggccctcgag cgaccgccca cgatccccgc aaagcgccgc
    10501 ccaacggcaa actgctcttg gctcgagctt ggcggggtga cgcaccacaa cttacgtggg
    10561 gtcgacgtac gcttcccgtt ggagcgcttg gttgccgtca ctggcgtcag cggttcgggc
    10621 aaaagctctc tggttcgcga ggtcttgctg cgcgcgaccc gggaggccgt cgggatggtg
    10681 aacgagatcg cggcaggtca gcacgatcgg attcgcggct tcgagacctt gaagcgcgcc
    10741 atcgaaatcg accagagccc gattggccga accccgcgct ccgtacccgc tacttacatc
    10801 gggatctgga acacggtgcg tcagttcctt gcaggcaccc ccgaggcgcg cgcccgagga
    10861 tatacggcga gtcgcttctc gttcaacgtc gaagagggcc gctgcccgga atgcaagggg
    10921 cagggggcga tccacgtcga gatggccttc ctgcccgatg tcgacgtgcc ctgcgaaagc
    10981 tgcaacggaa tgcggttcac accggagacg ctctcggtca gatggcaggg tctcaatgct
    11041 ggagagatcc tggagctcga ggtcgccgaa gcggtcgagg tcttctcccc agtgcgaacc
    11101 atccgtcagc ctttgcagct actcgacgag ctgggcctcg gatatctgca cctgggtcaa
    11161 gcgtcgaaca ccttgagtgg cggcgaagcc cagcgcatca agctggtttc ggagctcgca
    11221 gccgggctca acacgggccc cacactctac gtgatggatg agccgaccac tggtcttcac
    11281 cgtgatgacg tgaaccgcct cctcggcgtc ctcaaccgac tggtcgatcg aggcgacacc
    11341 gttgcggtca tcgagcacca tccggacgtg atgctcgcag ccgactggat cgtggacctc
    11401 ggacccgagg gcggaatcca cggaggtcgt gtggtggccc agggcacgcc cgaaaccatc
    11461 gccaaacgag cccgaagcca cacggggcag gtgattcggc gagagatgaa aagggcttag
    11521 ccgctctcag tggctgccga gctctttcaa gtcgggcccg cagtagagcg gggtggcgag
    11581 ccgctgctga aggtcagcgg cggtaaggcc atggcgaact tcacgcacac gaacgccatt
    11641 cgggccgaaa tcgaagaccg cgaccgggca gatgagccgg ctaagctgaa cccctccttc
    11701 gacttccgtg cggaacagcg agctgagctc gtccagcgtg gagaggctga gaccgacgac
    11761 cgggcagctc aagtccgagg gaacggcatc ggtcgccgcg cggcctctcg tcgtcaccgc
    11821 gcgcacctcc acgaatgcga tctcggcagg ctccgtaccc gttccggtgc cgtgcgcttc
    11881 cagcgcgagc ctcaggacct gaggcgagcc gttaccgaac cagatcccct cccgcgcatc
    11941 gcactcctct gcggcgcggg agagcaggcg ctcgagatca gctgcgtgct cgggcgatgg
    12001 catctgtgaa gcgccggaag aggtagcgcg agtcgtgtgg accagcggcc gcctcagggt
    12061 gatactgaac gctgaaggcc ccggtttcga ggtgttcgat cccttcgcag gtgccgtcgt
    12121 tcaagtgcag atgggtcact tcacacttgt tcttgagtga gtcgacatcg acgcagaatc
    12181 cgtggttctg ggtggtgacc tcgacgcgcc ccgtcttgag atctttgacc ggctggttga
    12241 gcccgcgatg tccgaacttc agtttgtagc tctcgccacc gagcgcgagg ctcatcaact
    12301 ggtggccgag gcagatgcca aaaatgggac gctggcccag aagcgcccgc acggtcccga
    12361 tcccagcggt gacagcggcc gggtcgccgg ggccgttcga cagaaacacg ccgtccggat
    12421 cgagcgcgag cacctcttcc gcggtcgccg ttgcgggcac gacggttacc ttgcatccct
    12481 cgtcgactaa acagcgcagg atgttgagct tcactccgaa gtccatcgcc acgacatgcc
    12541 acggtcgccc gccctcacgc tgctgcgacc agactccgct cccctcggtc catgaatagg
    12601 ttttggcggt ggtcacctcg cccgtgagat cgagaccggt catcggggtt gcggcctttg
    12661 cgcgatcatg gagtgtggct gggtcttccg tgcccaacgc agcgagctgc gcaccatggt
    12721 cgcggatgtg ccgagtcagc gttcgagtat cgactccgct gatcccgacg atcccttgcc
    12781 gtcgaaggta agcgtcgagt gactccttgg tgcgccagtt gctctccact gcggacaatt
    12841 cgcggacgac gaatcccctg agggcaggtc gtttggcttc gccgtcttcg agattgatgc
    12901 cggtgtttcc gatttgcggt gccgtcatcg tgacgatctg accgcagtac gaggggtcgg
    12961 tgagcacctc ttcatagccg gtcattcccg tggtgaacac ggcttcgccc gtggtggcgc
    13021 cttcggcacc aaagcgggtc cccgagaatc gggttccatc tgcgagtacg aggtgtgcta
    13081 cttcggtcat gttcagagct cgtgcacgac tttgcccgca acaatcgtca tgatgggcct
    13141 gccagtcagc tcacaatcga gcatcggcgt gttgtgcgat ttggagcgta gcgcttcttt
    13201 gtcgagaatc cattgagttt ccggatcgag gagtgtgacg tccgccggac gcccctctcg
    13261 aagcgagcct ccttcaaaat cgggtatcaa gcgtgcaggc gccgtcgaca gcacctcgac
    13321 gaagcggagg ggcttgagca cgccatcgcg gaccagttgg aacatcgacc cgatcgccga
    13381 ttcgaggccg ttgatgccca ccgaggcggc ctcgaactcg caatccttct cgagatccgt
    13441 gtgaggggca tgatcggtgg cgatacaatc gatcgtgccg tcggccaacg cctctcgaag
    13501 cgactcgcga tcctcgagct cgcggagggg agggttgacc ttgcaataag tgtcgtaacc
    13561 gagaagtgct tcatcggtga aaagcagatg gtgaggcgtg acctcggcgg agacacgcag
    13621 gcctcgggac ttggcctcac ggatgagtcg aaccgctcca aagctcgata tgtgggccac
    13681 gtggtatcgc gcgccggtgg tctcggccag gatcagatcc cgtgcgacga tgatgtcctc
    13741 ggctgcgcgc ggccagcctc gcagcccgag ttgcgtagag cgcgcgccct cgtgcatctg
    13801 agcgccctcg gtgagatcgt gatcctcaca atgctggctc accagcatat cgaaggtgcg
    13861 cgcgtactcc atggcgtgac gcatgacggc cgcattcatg acgcaacggc cgtcgtcgct
    13921 gacgccgatg gcgcccgcgt cacgcaggtc ggccatctcg gtgagctcgg atccccgctg
    13981 tcccttggtg acggcgccaa agggcaacaa gcgtgttccg ccgtgctgct gggcgcgcgc
    14041 gagcatcatc tcggtgatcg cacgggtatc gttcgccggc ttggtgttcg gcattggaca
    14101 aaccgcagtg aacccccctg cagcggccgc atccaaaccc gacccaatgt cctctttgta
    14161 ttcctgcccc ggctcgcgca gatgcacgtg aaggtcgacg aagccagggc atacccaacg
    14221 ccctgaggcg tctacgatcc gcacatcatc gccagcctca tatgcggctc ccgcgtcgcg
    14281 gcccacgctc ttgatccggc ctgcctcgat gacgacgtcc gcgatcgtgt cgagcccgca
    14341 tgccgggtcg agaacgcgtc cgccccgaag gacgatcagc tggttcaaca tagcggtccg
    14401 cgcttacgtg acggacccaa accagtcaac ccggtcagcc ggccttgcgc tgaagatcgt
    14461 cgtcgtccgc ggctgcgacg gtaccgacag aagaagacag cttctcatgc gtcttcttgt
    14521 cttcgacgcg cttcttcatc gtcatcacct tgcggtagac cgacgcgaag tccttgttgc
    14581 gcgtcgccat cgagggggtg ccgccacgca ggagggtctg gaggatgaat tcgagaacct
    14641 gaatctcgcc ttcggagaaa ctattgctga tgcgcatgcg atcggatcta ccaccggtcc
    14701 cgctgcatga caatcagtga cgtgcaacgt agtgccatga gatgtcatat tctttctagg
    14761 tttgcagtcc aaatccccga cgaacaacga ttaatagcgc agcctatccg gccatagacg
    14821 atcgcaccga agcggccagg agggtcaacg aggttgacaa acgctccaag aaacgcggct
    14881 accagcgcgg gctcagttgt cggccgcggt cacccggacg atctcgtcga tcgtcgtgac
    14941 cccctgaagg actttgttga ggcccgccct gcgaagggtg gcgacgccgt cccgaatcat
    15001 ttcgtctttc acctcggccg tcgatccgcc ttggaggacg atctcgcgga ggcgatcccg
    15061 gaacggcatc acctcgtaga gcgcgactcg tcccttgtag ccggtgtcgt tgcagactct
    15121 gcatccagtc cctcggaagg ccaccgcgtc ggcaatctcg ccgtctttga agcccacgtc
    15181 gatcaggacc tgctcgtcag ttggcacctg aaccttgcac tcttcgcaga tcttgcgggc
    15241 aagccgttgg gcgagcacga ggttcaccga tgcggtcacg aggaacggct ccacgcccat
    15301 gttgagcaga cgcgtgacgg tgctcggcgc gtcgttggtg tgcaaggtcg ataagaccaa
    15361 gtgacccgtc aacgccgcct tgaccgcgat ttcggccgtt tcgaagtctc gaatctcacc
    15421 gaccatgatg atgtcggggt cctgacgcag aaacgacctc agcgccgcgg cgaaattcaa
    15481 tccgatgtct tcgtgcatct gcacctgatt gatgcccatc aagttgaact cgatcggatc
    15541 ctcggcagtg ctgatgttcg tcccgacttc gttcagatcc gacatggcgg agtagagtgt
    15601 cgtcgtcttg ccggaacccg tcggcccggt gaccagcacc atgccgtatg gctggtgaat
    15661 cgcatccctg aaatccgaca gaggcttctg ctcgaatccg agcttggtca tgtcgagctg
    15721 caagttcgac ttgtcgagaa gacggagaac gatcttctcg ccccagagtg tgggtaccga
    15781 cgaaactcgg tagtccatct cgcggccctt gccgagcttg agcttgatac ggccatcttg
    15841 cggcaagcgg cgctctgcaa tgtcgagtga ggacatgatc ttcaaacgcg aggcgatggc
    15901 gttcttcagc ttgatgggcg gcgtcatctc ctcgtgaagc accccatcca cacggtatcg
    15961 gacgcgaagg acgcgctcgt agggttcgac gtgaatgtcg gaagcccctt gcttgatcgc
    16021 gttcaacaga atcgcgttgc atagtctgat gacgggcgcg tcctcgctgg ccctctcgag
    16081 gtcgagtaga ttctcatcgt catcgctggt agcgacttcg atctccgact cgtcgaagcc
    16141 ctccatgatg tcttcgtaag atatctctgg ctgtgcatac gccttctcga gcgcggacaa
    16201 gatcgcgctc tccgacgaga cca
//
LOCUS       DASRZF010000158         6840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_106047, whole genome shotgun sequence.
ACCESSION   DASRZF010000158 DASRZF010000000
VERSION     DASRZF010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 6840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6840
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106047"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..834)
                     /locus_tag="VFG22_07080"
     CDS             complement(25..834)
                     /locus_tag="VFG22_07080"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET6416042.1"
                     /translation="MYSRVAAITGMPRSGTSWIGQIVDSSPDVQYRMSPLFSYEFKNQ
                     IREGATREDWERVLRGSYVSQNDFMSQTIRRKAGEYPTFGLKNANPSLLVIKYNRFQN
                     LTEEMLELLPELKMLAIVRHPCGAIHSWLTAPKEFPPEADPLEHWRSGAAKKSGYGDF
                     FGFDDWCWVTRLHLRLARERPDQFRLVRYEQFVDDAERQTAALFDWLELEYSEQTADF
                     LRESQTSAVSGDYSVFRAPSVKDRWRSELQPAIRDAILSELGGTDLADLLR"
     gene            complement(839..1537)
                     /locus_tag="VFG22_07085"
     CDS             complement(839..1537)
                     /locus_tag="VFG22_07085"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6416043.1"
                     /translation="MIDWRHYWEDFPQKFAEGDFCRQVGRTANGGIPTPARELNVVID
                     EIVELLDLVPGDRLLDLCCGNGLLTVRLAQVCTNVVGVDFSNPMIQIAREHHSAPNAE
                     YVEGSVLDLDQILDTAAFDKMCMVESLAYFDPGQLSRILRGLSSVAAARATILFSGVL
                     DDDAKWSFFDTAERRAVYEQQRREGREIMGRWWTRSEIAWAAAENGFQAKILPQSPRL
                     NTAHYRFNVLMERG"
     gene            complement(1561..2493)
                     /locus_tag="VFG22_07090"
     CDS             complement(1561..2493)
                     /locus_tag="VFG22_07090"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HET6416044.1"
                     /translation="MSIEKEPSDAENFVRHRQFCTRARDGFTHLLRQTWPGGKSRILL
                     PAYIGHSPLEGSGIFDPLIALGIPYGFYAIDHALHFDLASLERELARQDVFGVLAIHY
                     FGSRQPELGEMKRLCERGGVILIEDCCHCIERPGQPIGDTGDYALYSIHKVLPCEDGG
                     ILQHARNAPDPGALPAELVCSDLPVRLWRDAAFHAIAERRVNNYRQMSTELQGLSGIT
                     PFWDRLQEGAVPLNFPVLVESADRFEVYKRMRAMGVGVVALYHTLVAAIEPDRFPVSH
                     EISRKILNLPIHQDVGSEDVSRIVATLSNALKSS"
     gene            complement(2490..3215)
                     /locus_tag="VFG22_07095"
     CDS             complement(2490..3215)
                     /locus_tag="VFG22_07095"
                     /inference="COORDINATES: protein motif:HMM:NF020463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HET6416045.1"
                     /translation="MRLGIMQPYFAPALGYFDLISLCDKWVVFDVADYRPKSWMVRNR
                     ILHPTSGWQYIKAQTGKVSRGTPIRDVRLVANREWRDLIIWQLEHYRRRAPFFEDTRR
                     LVEHSLDSDTSSLAQLSVRILSEFCARLEIDFSPLFFSEMDLPIGPIAGPGDWALRIS
                     EAMGATEYVNPPGGRDLFDAQAFADAGIKLSIREFEDLGYEPRGYEHVPMLSIVDVMM
                     WNSPERVIEHLSRERRKFESAEP"
     gene            3344..4567
                     /gene="egtB"
                     /locus_tag="VFG22_07100"
     CDS             3344..4567
                     /gene="egtB"
                     /locus_tag="VFG22_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HET6416046.1"
                     /translation="MTHDRLASDFSRVRAQTEALAAPLSDEDQCVQSMADASPTKWHR
                     AHTTWFYETFVLGAAGIDGLSPSAYAVLFNSYYNTVGAQYHRPSRGLLSRPSADDVSA
                     YRQRVDAAVLDLLAAEPSAELLRRVEVGLHHEQQHQELILTDIKDLFSCNPLEPSYID
                     RAPDSGVRSSPPPMRWQSNEGGVVSIGCGPSRFHFDNEGPVHEVLLRSFELGQRLVTN
                     AEYLAFIDDGGYERPELWLSAGWDTVQKHGWQAPRYWRRDQGSWSTFTLRGRREVRQE
                     EPVVHVSYYEADAYARWSGSRLPTEQECEAANSDPEPAPLCGREPLVHPTTPSPYGAA
                     WQWTSSSYSAYPRYRTAEGALGEYNGKFMCGQYVLRGSSSVTPVGHSRRTYRNFFPPE
                     ARWQFSGIRLARDGG"
     gene            4572..5561
                     /gene="egtD"
                     /locus_tag="VFG22_07105"
     CDS             4572..5561
                     /gene="egtD"
                     /locus_tag="VFG22_07105"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HET6416047.1"
                     /translation="MIAFDPRSTEERVRIVDLHPDLGDIRAEATDGLRRNQKKLPCKY
                     FYDALGARLFEAITELPEYYPTRTELGIMNASVHEMAEAIGGHATLVEFGSGVGLKTQ
                     KLLDALIEPTGVVLVDISRAALEASAYQLADRYDDMEVIAVCADYTQALRLPRPNRPS
                     ARTVAFFPGSTIGNFTHPEAISFLGRVAELVGRGGGLLVGIDRRKDTEVIENAYNDSL
                     GVTAAFNLNILRRLNESGANFDLGKFRHHAPFLEHEGRIEMRLISEVEQSVRVGTSSF
                     SLQAGEHIVTEHSHKYDLSQFEDMARSAGFRLTKHWSDARDWFSVCFLEVDDD"
     gene            5554..6111
                     /locus_tag="VFG22_07110"
     CDS             5554..6111
                     /locus_tag="VFG22_07110"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HET6416048.1"
                     /translation="MTERSVLDSLALLFEETARAHHEAFAATEGVDEDWALWYAARIQ
                     DPLAKLTGGKLSKAEIVVALVDAEAEHQARFPDELWPRSYAKYFCERYTCEPDETLAL
                     YYTPSCPFCVRVLRVIDELKIEVELRDIWINETYRSELREARGRTTVPVLRCTSASKD
                     RWMPESADIVRYLRGRFATTSNSAE"
     gene            complement(6066..>6840)
                     /locus_tag="VFG22_07115"
     CDS             complement(6066..>6840)
                     /locus_tag="VFG22_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416049.1"
                     /translation="ACGVVETSCLSKGCECGVAVNEASLIHYQRKLAECRGQEKLAVC
                     DFACETPFGRCFKGACVLTSEPPDLFRRGRSVQALCEASRGTYVGCPECAPNERCESC
                     LPCECPSSHRWTKKGCRAVVQTEARDIRVEVRPASLSFEDKLKARVHNDSKREIWLKT
                     ICGTPFYRARKKEDAWEKGYEPFHAAKCRVGSVELRPGASRPFVVTNLAKFEDPSGQS
                     ASAGTYRFELTYTDGNESFRYSAPVYSAEFDVVANRPRK"
BASE COUNT         1390 a         2033 c         2024 g         1393 t
ORIGIN      
        1 tttcccagta ttccggttca ggggtcatct aagcagatcc gcgagatccg ttccgcccag
       61 ctctgaaagt atcgcgtcac ggatcgctgg ttgaagctcg gaccgccagc ggtccttgac
      121 ggatggtgcg cgaaagaccg agtagtcgcc agaaaccgcc gaggtttgcg actcgcgcag
      181 aaaatctgca gtctgttcgg agtactcgag ctcgagccag tcgaacagag cggcggtctg
      241 ccgctctgcg tcgtctacga actgctcgta acgaaccagc cggaattggt ccgggcgctc
      301 gcgggcgagg cgcaggtgca ggcgcgtcac ccagcaccag tcgtcgaacc cgaagaaatc
      361 cccatatcca gatttcttgg ccgctccgga gcgccagtgt tcgagcggat cggcttcggg
      421 cggaaattcc ttgggtgccg tcagccagga gtgaatcgct ccacagggat gtcgaacgat
      481 tgccaacatc ttgagttcgg ggagcagctc gagcatctcc tcggttaggt tctgaaacct
      541 gttgtacttg atgaccagta ggctcgggtt cgcgttcttc agtccaaagg tggggtactc
      601 gccggctttt cgcctgatcg tctggctcat gaaatcgttc tgagaaacgt acgaccctct
      661 tagaaccctc tcccaatctt cgcgtgtggc cccctctcga atctggttct tgaattcata
      721 ggagaacagt ggggacatcc ggtattgaac atctgggctg ctatcgacga tctgaccgat
      781 ccagctggtg ccggaacggg gcatacccgt aatcgccgcc actcgggagt acacagggct
      841 atcctcgttc catgagcacg ttgaacctgt agtgtgccgt gttcaggcgc gggctctgcg
      901 gcaagatctt ggcttgaaaa ccgttttcgg ccgcagccca tgcaatttca gatcgagtcc
      961 accatcgacc catgatctct cttccttctc tccgctgctg ctcatagacc gctcgccgct
     1021 ccgcggtgtc aaagaagctc cacttcgcgt catcgtccaa tacccccgag aacagaatgg
     1081 tagcgcgcgc cgcagccacc gacgacagtc cacgaaggat tcgtgacagc tgtccaggat
     1141 cgaagtaggc cagcgattcg accatgcaca tcttgtcgaa cgcagccgtg tccaggattt
     1201 gatcgagatc gagcacggat ccctcgacgt attctgcatt gggggccgag tggtgttcgc
     1261 gtgcgatttg gatcatcggg ttggagaaat cgacgccaac cacgttcgtg catacttgcg
     1321 cgagccgtac cgtcagcagt ccgtttccgc agcagagatc caatagacgg tctcccggca
     1381 cgaggtccaa cagctccacg atctcgtcga tgacgacgtt cagctcgcga gcgggcgtgg
     1441 gtatcccgcc gttggccgtc cgcccgactt ggcggcagaa atccccttcg gcgaacttct
     1501 gcgggaaatc ttcccaatag tggcgccagt cgatcatgac ccctgagtcc tcatcattga
     1561 ctaactggat ttgagagcat tactgagcgt tgccacgatc cgcgagacgt cttcgctccc
     1621 aacgtcctga tgaatcggta ggttgagaat tttccgcgaa atctcgtgcg agactgggaa
     1681 tcgatcaggt tcgatcgccg cgaccaaggt gtggtagagc gcgacgacgc ccacacccat
     1741 ggcgcgcatt ctcttgtaga cctcgaatcg gtcggcgctc tcgactagca cagggaagtt
     1801 cagcggtacc gcgccctctt ggagtcgatc ccagaaggga gttatcccgg acagcccctg
     1861 aagctcggtg ctcatctgtc gatagttatt cacgcgtcgt tcggcaatcg catggaatgc
     1921 tgcgtcacgc cagagtcgca caggcaaatc ggaacagacg agctcagccg gtagagcacc
     1981 ggggtctgga gcatttcggg cgtgctgcaa aatacctccg tcctcgcacg gaaggacctt
     2041 atgaatcgaa tagagagcat aatcgccagt gtctccgatg ggttgccctg gtcgctcgat
     2101 gcagtgacaa cagtcctcga tgagaatgac accaccccgt tcgcagagtc tcttcatttc
     2161 accaagctcg ggttgacgac ttccgaaata gtggatcgcg agaacgccga atacatcttg
     2221 cctcgccaat tcgcgttcga ggctggccaa gtcgaaatgg agagcgtggt cgatcgcata
     2281 gaacccgtac gggatcccga gtgcgatgag agggtcgaag atccccgagc cctcgagtgg
     2341 ggaatggccg atgtacgcag ggagcagaat cctggacttt cctcccggcc aggtttgacg
     2401 aagaaggtgt gtgaagccgt ctcgcgcgcg agtgcagaat tgcctatggc ggacgaagtt
     2461 ctctgcatcg gaaggctctt tttcgatgct catggctcgg ccgattcgaa cttcctgcgt
     2521 tcgcgcgaaa gatgctcgat aaccctctcc ggtgagttcc acatcatgac atctacgatc
     2581 gagagcatcg gaacgtgctc gtacccgcgg ggttcgtaac cgaggtcctc gaactctcga
     2641 atgctgagtt tgattccggc atccgcgaag gcctgcgcgt caaatagatc tcgtcctcct
     2701 ggtggattta catattcagt ggcgcccatc gcctcgctga ttctcagtgc ccaatcgcca
     2761 gggcccgcaa taggaccgat gggtaaatcc atctcggaaa agaacaacgg ggagaaatcg
     2821 atctcgaggc gggcgcaaaa ctcggacagg atccgaacgg acagctgcgc caaggagctc
     2881 gtatccgagt cgagactgtg ctccacgagg cggcgcgtgt cctcgaaaaa tggcgctctt
     2941 ctccggtagt gttccagctg ccaaatgatg aggtcgcgcc attcccggtt ggcgacgagc
     3001 cggacgtccc ggatcggcgt tccgcggctc actttgcccg tctgggcttt gatgtactgc
     3061 cagccggaag tcggatggag aattctattc ctcaccatcc agctcttggg gcggtagtct
     3121 gcgacatcga aaaccaccca tttgtcgcac aacgagatta ggtcgaaata gccaagcgcc
     3181 ggcgcgaaat acggctgcat gatccctagt ctcatcttga ttcgggtggc ggtcaggccg
     3241 ccattcccca agtcaaaaca atagcatggg agtgacgcgc ggcctctttt ttggtaacac
     3301 gaacgctctt gacccgttct aggcggtacc tatggaaggt cagatgacgc acgatcgact
     3361 tgccagcgat ttcagccggg tgcgtgcgca aaccgaagct ctcgctgcgc cgctctccga
     3421 tgaggatcag tgcgttcaga gcatggcaga cgcgagcccc acgaagtggc atcgcgcgca
     3481 tacgacctgg ttctacgaga ccttcgtctt gggggccgca gggatcgatg ggttgtcgcc
     3541 aagcgcgtac gcagttttgt tcaacagtta ctacaatacg gtcggcgctc aataccatag
     3601 gccgtcacgc gggcttctga gcaggccaag cgccgacgac gtgagcgcat atcgtcagcg
     3661 cgtggatgcc gcggtgctcg atttgcttgc cgcggagccc agcgcggagc tcctgcgtcg
     3721 cgtcgaggtt ggcctgcatc acgaacagca gcaccaggag ttgatcctga ccgacatcaa
     3781 ggatctgttt tcctgcaatc ccttggaacc gagttacatc gatcgagctc ccgactcggg
     3841 ggtcaggtct tccccacctc cgatgcgatg gcagagtaac gagggcggtg tcgtttccat
     3901 cggctgcggc ccgagtcgat ttcatttcga caacgaaggc ccggttcacg aagtgcttct
     3961 tcgaagcttc gagctcgggc agcgcctcgt gacgaatgcc gagtacctcg cgttcatcga
     4021 cgatggcgga tacgagcgac ccgagctctg gctcagtgct ggttgggaca ccgtgcagaa
     4081 gcacggctgg caagcgcctc gatattggcg aagggatcaa ggttcgtgga gcacgttcac
     4141 gctccgaggc cgacgggagg ttcgccaaga agagcccgtg gtgcacgtca gttactacga
     4201 agcggatgcc tacgcgcgat ggtccggttc tcgcctcccg acggagcagg agtgtgaggc
     4261 cgcaaactcc gaccctgagc cagcacctct ctgcggacgg gagccgctgg tgcatccaac
     4321 gactccaagc ccctatggcg ccgcctggca atggacgagc tccagctact ccgcttatcc
     4381 gagatatcgc acggccgagg gcgcgctcgg cgagtacaac gggaagttca tgtgcggcca
     4441 gtacgtcttg cgaggcagca gctctgtaac cccggtcggc cactcacgcc gtacctaccg
     4501 aaacttcttt cctcctgagg ctcgctggca attcagcggc atcagactgg ctcgggacgg
     4561 cggctgattc gatgattgca tttgatccac gaagcaccga ggagcgcgtt cgcatcgtcg
     4621 accttcaccc tgacttgggt gacatcaggg ccgaggccac cgatgggctc cgccggaacc
     4681 agaagaagct tccctgcaaa tacttctacg atgcacttgg ggcgcggctc ttcgaggcga
     4741 taaccgagct tccggagtat tatccgaccc gcaccgagct ggggatcatg aacgcttctg
     4801 tccatgagat ggcggaagcg atcggcgggc atgccacctt ggtggagttc ggatccgggg
     4861 tagggctcaa gacccaaaag ctcctcgatg cattgatcga gccgacgggt gtcgtgctgg
     4921 tcgacatcag cagggctgcg ctcgaggcct ctgcatacca gctcgccgac cgatacgacg
     4981 acatggaagt catcgcggtc tgtgcggact acactcaggc tctgcgtctt ccccgtccga
     5041 accgaccgag cgctcgcacc gtcgctttct ttcctggaag caccattggg aacttcaccc
     5101 accccgaggc catctctttt cttggacgcg ttgcggagct ggtggggcgc ggcggcggac
     5161 tgctcgtcgg catcgacagg cgaaaagaca ccgaagtcat cgagaatgcg tacaacgatt
     5221 cgctcggcgt caccgcagca ttcaacctca acatactccg acgcctcaat gaaagcggtg
     5281 ccaatttcga tctcggtaaa ttccggcacc acgcgccctt tctcgaacac gagggtcgaa
     5341 tcgagatgcg gctgatcagc gaagtcgagc agtcggttcg agtcggaaca tcgagcttct
     5401 cgctgcaggc gggtgaacac atcgtgaccg aacactctca caagtacgac ctctcccaat
     5461 tcgaagacat ggctcgttca gcgggcttcc ggctgacgaa gcactggagc gacgcgcgcg
     5521 attggttcag cgtttgcttc ctggaagtcg acgatgactg agcgttcggt gcttgattcg
     5581 ctggcgttgc tcttcgaaga aaccgcgcgc gcgcaccacg aggcgttcgc agcgaccgag
     5641 ggggtggatg aagactgggc actctggtac gccgcccgga tccaggatcc gctcgcaaag
     5701 ctcacgggcg ggaagctcag caaggccgaa atcgtcgtgg cgctcgtcga cgcggaggcc
     5761 gagcatcagg cacggtttcc cgacgagctt tggccgaggt cttacgcgaa atacttctgc
     5821 gagagataca cctgcgagcc cgacgaaaca ttggctctct actacacgcc gagctgcccg
     5881 ttttgcgttc gtgtgcttcg agtgatcgac gagctgaaga tcgaggtcga gctcagagac
     5941 atttggatca atgaaaccta ccgtagtgag ctcagagaag cccggggccg aacgaccgtt
     6001 cccgttctgc ggtgcacgag cgccagcaaa gatcgctgga tgcccgaaag cgcagacatc
     6061 gtgcgctact tgcgcgggcg attcgcgacg acgtcgaatt ccgccgaata gacgggagcg
     6121 ctgtatcgga agctctcgtt gccatcggtg taggtgagct cgaagcgata tgtgcccgca
     6181 ctagcgctct gtccggatgg gtcttcgaac ttcgccaaat tggtcacgac gaagggtcgg
     6241 ctcgcgccgg ggcggagctc gacggaacca acacggcact tggccgcatg aaaaggttcg
     6301 tagcctttct cccatgcgtc ttccttcttt cttgcgcggt agaagggggt cccgcagatc
     6361 gtcttcaacc agatctcgcg cttcgagtcg ttgtggacgc gggccttgag cttgtcttcg
     6421 aagctgagcg aggcagggcg aacttcgact cgaatgtctc gtgcctcggt ctgaactaca
     6481 gcgcgacagc ccttcttcgt ccatcgatgg ctagacggac actcgcaagg caaacaggac
     6541 tcgcagcgtt cgttcggcgc gcattcgggg cagccgacat aggtgccgcg agaggcttcg
     6601 catagcgctt gcacgctccg ccctcttcga aagagatcgg gtggctcgct cgtgagaacg
     6661 caggctcctt tgaagcatcg cccaaaaggc gtctcgcatg caaaatcaca aacggcgagc
     6721 ttctcctggc cgcggcattc ggcaagctta cgctgatagt gaatgaggct tgcttcattg
     6781 acggcgacgc cacactcgca gcccttcgac aggcagctcg tttcgacgac tccgcaggcc
//
LOCUS       DASRZF010000159        12154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1828128, whole genome shotgun sequence.
ACCESSION   DASRZF010000159 DASRZF010000000
VERSION     DASRZF010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 12154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12154
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1828128"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="VFG22_07120"
     CDS             <1..514
                     /locus_tag="VFG22_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416050.1"
                     /translation="GTGGTGGTGGTGGTGGDPGVCAGGALLEPIPNCSPEPLPSTGDA
                     HADCVARINQFRWECQCLPPLARWVDGESCTDSNAEYDSVNGVHASFYDIPCGSGARA
                     QNECPGWPSNQQVITGCLQAMWDEGPEDGNPDTVNGHYESMASTTYTQVACGFFTTPT
                     GSVWGVQNFD"
     gene            526..1098
                     /locus_tag="VFG22_07125"
     CDS             526..1098
                     /locus_tag="VFG22_07125"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HET6416051.1"
                     /translation="MRGAKTTALTFVVLALGCGDFLDPQGVGGTTGTSGDPRFTAECS
                     NGPLNAPVANCRPDPLEGSGDAYADCVTRINQLRWECQCLPPLQRWNEAEGCADQHSQ
                     YDSTRSAHSGFRDEICSPSGWAQNECPGWSSTEHVITGCLQSMWDEGPGEPYSEHGHY
                     INMTNPSYSMVACGFYETPDGRFWSVQNFR"
     gene            complement(1118..1408)
                     /locus_tag="VFG22_07130"
     CDS             complement(1118..1408)
                     /locus_tag="VFG22_07130"
                     /inference="COORDINATES: protein motif:HMM:NF016265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+)-trafficking protein"
                     /protein_id="HET6416052.1"
                     /translation="MPEADPNRLVHCIKLDRELPGLAKPPFPNELGQLVFEKVSKEGW
                     DLWLKESVRYINTYRVDLSSREGSEFMIKQLRIWMGLEEGDLAATAWTPPSE"
     gene            complement(1445..2026)
                     /locus_tag="VFG22_07135"
     CDS             complement(1445..2026)
                     /locus_tag="VFG22_07135"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HET6416053.1"
                     /translation="MTHLGLPDLRAQLQGRTPRELAEHEKWAAVAMLLRDGERGPEVF
                     FIRRAEHPNDPWSGHMAFPGGRQDECDATLLHTAMRETHEEVGLDLSSGAEHIGQLDD
                     LQAIARGKPQETVIRPFVFEVHREWPLRVEEREVAEALWTPLLPLYRGEVDTIRPYQW
                     HGTQIDFPAYDVEGRIVWGLTYQMLRSFFRILG"
     gene            complement(2032..2274)
                     /gene="purS"
                     /locus_tag="VFG22_07140"
     CDS             complement(2032..2274)
                     /gene="purS"
                     /locus_tag="VFG22_07140"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HET6416054.1"
                     /translation="MKANIFVTLKREVLDPQGDAVRRSLSSLGFEEVKSARVGKLIEL
                     ELRGSDKASAESHIQSMCEKLLANPVIEDFHYEILD"
     gene            complement(2271..3242)
                     /locus_tag="VFG22_07145"
     CDS             complement(2271..3242)
                     /locus_tag="VFG22_07145"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HET6416055.1"
                     /translation="MTISAEALREGLAKTLDGTNFETLGHKYEGKVRDCYTADGKRYI
                     VVTDRISAFDRVLGTLPYKGQVLNGLAAWWFEETKGIVPNHVIDVPDPNVMVGIECEP
                     LPVEMVVRAYITGVTSTSIWTHYEKGTRVFCGHELPDGLKKHQKLPDPILTPSTKAEK
                     GDHDVSVSREEILAMGRISEEDFDEAASFAMALFAHGQKICAERGLILVDTKYEFGKT
                     PEGKIVIIDEIHTPDSSRFWYANSYPQRFEAGEDPESFDKEYVRRWLASNGYRGDGPI
                     PTIPDEVRVEASRRYIQACDEIRGTAFEPDTSDPLPRIAKNLGLEGA"
     gene            complement(3244..4545)
                     /gene="purB"
                     /locus_tag="VFG22_07150"
     CDS             complement(3244..4545)
                     /gene="purB"
                     /locus_tag="VFG22_07150"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HET6416056.1"
                     /translation="MIPRYTPAEFQALWSQKRKYEAWFDVEIAGCRAMENAGIVPKGT
                     ADKVGAFRAQLDADAIDEIEKVTRHDVIAFLTHVEGLAGEPARWLHRGMTSSDVLDSS
                     LALLLVEATDGLLVRLDAVLEAMRGRVEEHRRTPAIGRSHGIHAEPVTFGLILAGHYA
                     ELARGRQRLLVAREEIAVGKIAGAVGTYAHLTPAIEAEALSSLGLRPETAATQVVARD
                     RHAAYVATLGIIAAGIERFSTNVRHWQRTEVGEAEEHFKKGQKGSSAMPHKRNPVLTE
                     NLCGLGRVVRAAVVPGLENVALWHERDISHSSAERMMLPDATSTLAFMLERMRAVVAD
                     LVVYPERLRQNLDQTGGLWASEGILLALVGQGLGRQDAYVHVQRNAMKAFEGEGDFRT
                     NLREDAEIGKYLSSDDIDHEFDLNHALAHVDTIIDRVLKGD"
     gene            complement(4647..4949)
                     /locus_tag="VFG22_07155"
     CDS             complement(4647..4949)
                     /locus_tag="VFG22_07155"
                     /inference="COORDINATES: protein motif:HMM:NF019491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HET6416057.1"
                     /translation="MIERIVLFKLKDEYCNDAARAEFAERTQADLSGLDNVQSVTVGI
                     PADEASEASWDISIVIRFANMQDVQRYISDPAHRAYVDQYATPRIQVRKAWNFRIA"
     gene            complement(4954..6006)
                     /locus_tag="VFG22_07160"
     CDS             complement(4954..6006)
                     /locus_tag="VFG22_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET6416058.1"
                     /translation="MLPVPPKNTGPVRDAHRFDEARLDAWMSDNVEGYSGSLQVAQFK
                     GGQSNPTYWLADRERQYALRKKPPGKLLQSAHAVDREYRVMRALGETDVPTAKMYALC
                     EDDSVIGTSFFVMEYVAGRIFWNVQLPDLDPEERRAIYEELARVLAAIHSVNLDAVGL
                     SDYGRPDAYVARQVKRWTKQYLASQTANVEPMNTLIEWLPANIPDDEATALVHGDYRL
                     DNLIFHPTEPRALAVIDWELSTLGHPLSDLAYTCMLYDVMLPKIGGLAGVSFQKTGIP
                     PEEAFVARYCELVGRDGVPDLNYYKAFSIFRLAAIAQGVYKRSLDGNASSTEAAMFGA
                     AVPHLAGIGCRLAGIG"
     gene            6067..7278
                     /locus_tag="VFG22_07165"
     CDS             6067..7278
                     /locus_tag="VFG22_07165"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6416059.1"
                     /translation="MDFSTPPELEDLLSRAQELMEAEVYPLEASARQSFEEVLPAANE
                     ARERCKAAGLWAPQMPTEYGGMGLDFLPHAHMSELLGRSPLGHYIFGCQAPDAGNMEI
                     LHKFGTDEQKENWLRPLAAGKIRSCFSMTEPDRAGSNPTWMETTAVRDGDDLVINGRK
                     WFTTGGHLADFAIVMAVTNPDAAPHMRASQIIVPTSNPGFHHIRRLSIMGDVGYGWQS
                     HSEVAYEDCRVPANNLLGQEGFGFLIAQERLGPGRIHHCMRWLGICERSFDLMCERAA
                     RRMVAPNRPLGSQQAVQHWIAESRAEIDSARLMVYQAAWKIDREGTRAARNEISAIKF
                     MVAGVLQKVVDRAIQVHGGLGMTDDTPLAWFFRHERAARIYDGPDEVHKSALARSILK
                     KYGIELGSQGS"
     gene            7289..8740
                     /locus_tag="VFG22_07170"
     CDS             7289..8740
                     /locus_tag="VFG22_07170"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HET6416060.1"
                     /translation="MRWARYLRLSFGGFLIAGALLCAVQVTFGQKPDKEADDSGYRPA
                     PSANVEGDVQALPIDAPAGSEVVVVAIDRTVELGLAAFVRRAIETNPDAAAIVLEVNT
                     LGGRVDAAIQIRDALLEAKPRTVAFIHPRAISAGALISLACDNIIMTDGATIGAATPI
                     QAGPGGQAEAVGEKMVSYMRAEMRTTAEANGRRGDIAEAMVDSEVVIDGIVEAGKLLT
                     LDTDQALKMGMADAKATDLADVMKILTLESPEIIEVELNWGEEIARWLTEPTVSGLLL
                     SVGMLGLMVAFYTRSVGAFTLVGFVSLALFFGGHAVVNLVGWEEALLFLSGLVLVVVE
                     IFFVPGLGVPGVLGLIFVIAALVLALIGIPIDVSFKTGVLADAMTRVLLSLLGAFVLA
                     LVAMRLLSRTMAGKGFILENAETGFLSAPSASNLVGQVGEALTDLRPAGKIVVGGKRH
                     EATSEREFVARGARVQVIGKDGPTLIVRPEREA"
     gene            8737..9207
                     /locus_tag="VFG22_07175"
     CDS             8737..9207
                     /locus_tag="VFG22_07175"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HET6416061.1"
                     /translation="MTPVIIVVVLVVGLLLLFTEVAVIPGFGVAGALGLLALGAGAVA
                     AWTELGPFWGGISTGFSFVAAGFMLFWLPKSRMGRMMVLEHSQADAVSQRDQSELLGR
                     RGITVTPLRPIGRVRFGSSEVDVESEGEYIASNLEVEVITVEGSRVVVRIPDAN"
     gene            9220..10206
                     /gene="floA"
                     /locus_tag="VFG22_07180"
     CDS             9220..10206
                     /gene="floA"
                     /locus_tag="VFG22_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237964.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HET6416062.1"
                     /translation="MQFAPLALIFLLFIVIFVLFVWLIPVRLWIAAWSSGARVGFGEL
                     IGMRLRRVSPGAVVNPRIAAVKAGIDTETNILESHYLAGGNVQRVVNALISADKAGIS
                     LGFSQAAAIDLAGRDVLEAVQTSVNPRVITTPKITAVAKDGIQLLVITRITVRSNIDR
                     LVGGATEETVVARVGEGIVSTIGSSLNYKEVLENPDSISKNVLARGLDSGTAFNILSI
                     DIADIDVGTNIGAKLMTEQAEADKQVAQARAEGRRALAVAAEQEMRAKVEEMRAHLVE
                     AEAKIPEAMADALRAGNLGVMDYYNLRNVSADTEMRDSISKSTRGSNDDGEI"
     gene            10190..10342
                     /locus_tag="VFG22_07185"
     CDS             10190..10342
                     /locus_tag="VFG22_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416063.1"
                     /translation="MTAKSRPFAIKALLAQPRTFVARLIASDLIAKRGEGPLERKVLV
                     YKRRRR"
     gene            10348..11964
                     /locus_tag="VFG22_07190"
     CDS             10348..11964
                     /locus_tag="VFG22_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416064.1"
                     /translation="MEELLREHLGEIALLIYILYPLLKRWWDRQKKKREQRAGTPEPT
                     GQTPTRGEAPRPQTPVPHRPPSKPRIQPSVAKQSSEADLLEAARAERLRLEQASSALL
                     ARAEGEPRLARLVPAVRDDLLARLRVVERALSARPTVSSVVHETTVMRGLESLLRHLK
                     MMAQQRMLGRSAFLSEADAVADACYAPLIEFAGAQGLDLKTSQPLIVTGDWDLSIVPS
                     FASTRLAPLRLPRAFEQSLWGWPAIAHEVAHDFYYSLKGLEPSLRARLGLPSKVEMPM
                     SSAELDGAWLRNLFGAWLPEVVADTLGTTSLGPAYVETMLRAFRNPSSPQRTAAILQD
                     RSLIDEHPPPRLRLYMATRVLHHLGRHSEADALWARWEADHAEVGFYFLPLAGQWVGL
                     SDEALHSVADSIIDALVQRAWPELEGFHLLNIPGFAYQHAEHAKVTELSADLLHGAQL
                     DADVRWIIAAAVLAASAQPALHDPILRLARLSIAGRAQPAVAFVAPKRRHTSATIGET
                     LVASLRRPEAIKEAIILGAAIRPYKRPRWH"
BASE COUNT         2282 a         3833 c         3739 g         2300 t
ORIGIN      
        1 gggcaccgga ggcaccggag gcaccggggg caccggaggc accgggggag accccggcgt
       61 ttgcgccggt ggcgcactgc tcgaaccgat cccgaattgc agtcctgagc ctttgccgag
      121 cacgggagac gcgcacgccg actgcgttgc gcgcatcaat caatttcgct gggagtgtca
      181 gtgtcttcct ccgctcgccc gctgggtcga cggggagagc tgcacagaca gcaatgcaga
      241 gtacgacagt gtcaacggcg tacacgcgag cttctacgac atcccctgcg gcagcggcgc
      301 acgtgcgcaa aacgagtgcc cgggatggcc gtcgaatcag caagtgatca ccggctgcct
      361 acaggcgatg tgggacgagg ggccagagga cggaaacccg gacaccgtga acgggcacta
      421 cgaaagcatg gcgtcgacga cttacacaca ggtcgcatgc ggattcttca ccacgcccac
      481 cggaagcgtc tggggcgttc agaacttcga ttgagggggc actccatgcg cggagcaaag
      541 accacagcat tgacctttgt cgttttggcc ctgggctgcg gcgacttctt ggacccgcag
      601 ggtgttggag gaaccaccgg gacctctggc gacccgaggt tcacggccga atgcagcaac
      661 gggccgctca acgcgccggt ggcaaactgc cgacccgatc cgctcgaagg ctcgggcgac
      721 gcgtacgccg actgcgtcac ccgaatcaac caacttcgct gggagtgcca atgcctgccg
      781 ccccttcagc gttggaacga agccgaaggc tgtgcggatc agcattcgca atatgacagc
      841 acgaggagcg cgcattctgg tttccgcgac gagatctgct caccgagcgg ctgggcccag
      901 aacgagtgcc ccggttggag ctctacggag catgtgatca ccggttgcct gcaatcgatg
      961 tgggacgagg gccccggtga accgtactca gagcacggcc actacatcaa catgacgaat
     1021 ccctcgtatt cgatggtcgc ctgcggcttc tacgaaaccc cggacgggcg tttctggtcc
     1081 gtgcagaact tccggtagcg agacgaagcg gcgccggcta ttcgctgggc ggcgtccagg
     1141 cggttgcggc taggtcgcct tcttcgagcc ccatccagat tcggagctgc ttgatcatga
     1201 actcggagcc ctcccgcgag gaaaggtcga cgcgataggt attgatgtag cgcacgctct
     1261 ctttgagcca aagatcccag ccctctttcg agaccttttc gaagacgagc tggccgagct
     1321 cgttcggaaa tggcggcttt gcgagaccgg gaagctctcg atcgagcttg atgcagtgga
     1381 ccaggcgatt gggatcggct tcaggcatgc ttggagtcga acctagcacc cggacggttg
     1441 ctggctagcc cagaatgcgg aagaaagagc ggagcatttg atacgtgagc ccccagacga
     1501 tgcggccttc gacgtcatag gccgggaagt cgatttgggt tccatgccac tggtatggcc
     1561 tgatcgtgtc gacctcgccg cgatagagcg gcagcagcgg ggtccaaagg gcctcagcga
     1621 cctcccgctc ttcgacgcgg agtggccatt cgcgatgtac ctcgaagacg aaggggcgga
     1681 tgacggtctc ctgcggcttg ccacgtgcaa tggcctgcag gtcgtcgagc tgcccgatgt
     1741 gctccgcgcc gctcgacaga tccaagccta cttcctcgtg cgtctcacgc atcgcggtat
     1801 gcaacagggt ggcatcgcac tcgtcctgac ggccgccggg aaacgccatg tgccccgacc
     1861 agggatcgtt gggatgctcg gcccgccgta tgaagaacac ctccgggcct cgttctccgt
     1921 cgcggagcaa catcgccacg gcagcccact tttcgtgctc tgcgagctcc cgaggagtcc
     1981 tcccctggag ctgagctcgc aagtccggca ggccgagatg cgtcatgcca actagtcgag
     2041 aatctcgtag tggaagtcct cgatcacggg gttagccagg agcttctcgc acatggactg
     2101 aatgtgggac tccgcagacg ctttgtcgct accgcggagc tccagctcga tgagcttacc
     2161 gacgcgtgca gacttgacct cttcgaagcc aagggagctg agcgaacgac ggacggcgtc
     2221 gccctgcgga tccagcacct cgcgcttgag tgtaacgaag atgttcgctt tcatgctccc
     2281 tccaggccca ggttctttgc gatacgaggt aacggatcgc tggtgtcggg ctcgaacgcc
     2341 gtgccacgga tctcgtcgca agcctgaatg taccggcgtg acgcctcgac gcgcacctcg
     2401 tccggaatcg tgggaatcgg accatcgccc ctgtaaccgt tgcttgcgag ccagcggcgc
     2461 acgtactcct tgtcgaagct ttccgggtcc tcccccgcct cgaaacgctg cggatagcta
     2521 ttcgcgtacc aaaaccgcga ggaatccggg gtgtgaatct cgtctatgat gacgatcttt
     2581 ccctcgggcg tctttccaaa ctcgtacttc gtgtcgacga gaatcaaacc gcgctcggcg
     2641 caaatcttct gaccgtgcgc gaacaaagcc atcgcaaagc tcgctgcttc atcgaagtct
     2701 tcctcgctga tccggcccat cgcgaggatc tcttcgcgcg acacggatac atcgtgatcg
     2761 cccttctcgg ccttggtgct cggcgtcagg atggggtcgg gcagcttctg atgcttcttc
     2821 aagccatccg gtagctcgtg gccgcaaaaa acgcgcgtgc ccttttcata gtgagtccag
     2881 atgctcgtcg acgtaacgcc agtgatgtaa gcgcgcacga ccatctcgac cggcaagggc
     2941 tcgcactcga ttccgaccat gacgttgggg tccggaacgt cgatgacgtg attcggaacg
     3001 atccctttgg tttcctcgaa ccaccaagcg gcgaggccgt tgagcacctg ccctttgtag
     3061 ggaagtgttc ccagcacgcg gtcgaaggca ctaattcggt cggtgacgac gatgtagcgc
     3121 ttgccgtcgg ccgtatagca gtctcgtacc ttgccttcgt acttgtgacc aagcgtctcg
     3181 aagttcgttc catctagcgt cttggcgagt ccttctcgca acgcctctgc cgagatcgtc
     3241 atctcaatct cctttcagca cacgatcgat aatcgtgtct acgtgcgcga gcgcatggtt
     3301 caagtcgaac tcgtgatcga tgtcgtcgga cgagagatac ttcccaatct ccgcgtcttc
     3361 tcgaaggttg gtacggaaat cgccctctcc ttcgaatgct ttcatcgcgt tccgctgaac
     3421 gtgaacatac gcatcctggc ggccgagccc ttgccccacc agcgccaaga ggatcccctc
     3481 gctggcccag agacctccgg tctgatccag gttctgtcgc agccgctccg ggtagacgac
     3541 caaatcggcg accaccgcac gcatgcgttc cagcataaaa gcaagggtag aagtcgcgtc
     3601 ggggagcatc atgcgctccg ccgatgaatg gctgatgtcc ctctcgtgcc agagcgcaac
     3661 gttctcgagg ccgggcacca cagcagcgcg gacgacgcga cccaagccac ataggttctc
     3721 ggtcagtacg gggtttcgtt tgtgcggcat ggcgctcgag cccttctggc ccttcttgaa
     3781 atgctcctcc gcctcgccga cttcagtgcg ctgccagtga cgaacgttcg tcgaaaatcg
     3841 ctcgatcccg gcagcgatga tcccgagcgt agcaacgtag gcggcgtgac gatcgcgagc
     3901 gacgacctgc gtggctgcgg tttcgggcct caacccgagc gacgatagtg cctccgcctc
     3961 gatcgccggc gtgagatgtg cgtaggtgcc cacggcgccg gcaatcttcc cgaccgcgat
     4021 ctcctcgcgg gccaccagca gacgctgccg gcctcgcgcg agctctgcgt agtgtcccgc
     4081 caagatcaac ccgaaagtga cgggctcggc gtgaatgcca tgcgagcgac cgatggcagg
     4141 tgttcgccga tgttcctcga cgcgcccacg catggcttcg agcacggcgt ccaatcgaac
     4201 caagagcccg tcggtcgctt ccaccaagag cagagccaat gaagagtcga gcacgtcgga
     4261 tgaggtcatg ccccggtgaa gccaacgcgc aggttccccg gcgagcccct cgacatgcgt
     4321 gagaaatgcg atcacgtcgt gtcgcgtcac cttctcgatt tcgtcaatcg catcggcgtc
     4381 gagctgcgct cgaaatgcgc cgaccttgtc cgcggtccct ttgggaacga ttccagcgtt
     4441 ctccatggct cggcagccgg cgatctcgac atcgaaccaa gcctcgtact tgcgtttttg
     4501 tgaccagagg gcttggaatt ctgcaggggt gtatcgtgga atcatcgttg cgcggcgaag
     4561 ctagcagacc ggcagccccc gtgcgaccga gccccatcag tcagtcgaag agagcgatta
     4621 agccctctga aaaggggtct gacccttcat gcgattcgga agttccaagc tttgcgaact
     4681 tggatacgag gcgtggcgta ctgatccacg taggcgcggt gcgccgggtc gctgatgtat
     4741 cgctgcacgt cttgcatgtt ggcgaaacgg attacgatgc tgatgtccca gcttgcttcg
     4801 gatgcttcat ctgcgggaat accgactgtc acactttgta cgttgtcgag gccgctcagg
     4861 tctgcctggg ttcgttccgc aaattcggca cgcgctgcat cgttgcagta ctcgtctttc
     4921 aacttgaaca atacgattcg ctcgatcatc gccctatccg attcctgcca gtctacagcc
     4981 gatgcccgcg agatgcggaa ccgcggcgcc aaacatcgcc gcctccgtgg agctggcgtt
     5041 cccgtccagg cttcgcttgt acacgccttg cgcgatggca gccaagcgga agatcgaaaa
     5101 cgccttgtag tagttcaagt ccgggacgcc gtcgcgcccc acgagctcgc agtaacgcgc
     5161 gacgaatgct tcctcgggcg ggatgccggt cttctgaaag ctcacgcctg ccagaccgcc
     5221 gatcttcgga agcatcacgt cgtacagcat gcaggtgtag gccaggtccg agagaggatg
     5281 gcccagcgtc gagagctccc agtcgatcac ggcgagcgct cgaggctcgg tcggatggaa
     5341 aattagattg tcgagccgat aatccccgtg caccagcgca gtcgcttcat cgtccgggat
     5401 attggccggc aaccattcga tcaacgtatt catcggctct acgttggccg tctgtgaagc
     5461 gaggtactgc ttcgtccagc gtttgacctg ccgcgccacg tatgcgtcgg gccggccgta
     5521 gtcggagagc cccacggcgt cgaggttcac gctgtgaatt gcagccaaga cacgggccag
     5581 ctcttcgtag attgcgcgcc gctcctcggg atcgagatcc ggaagttgga cgttccagaa
     5641 gatacggccc gcaacgtact ccatcacgaa gaacgaggtc ccgatcaccg agtcgtcttc
     5701 gcaaagcgcg tacatcttcg cagtcggcac atcggtttcg cccaatgcgc gcatcactcg
     5761 gtactcacgg tcgactgcat gcgcggattg caggagcttg cctggtggct tcttcctcaa
     5821 tgcgtactga cgctcgcgat ccgcgagcca gtaggtcgga ttcgattgcc cgcccttgaa
     5881 ctgagcgact tgaagcgagc cggaatagcc ctccacgttg tcgctcatcc aggcatcgag
     5941 ccgcgcctcg tcgaagcggt gtgcatctcg aacgggtcca gtatttttcg ggggcaccgg
     6001 cagcatacgt cctcgctttc ggaaggtgcg gagcctatca tgctcaaccc aatccggagg
     6061 ctatcaatgg acttcagtac accgcccgag ctcgaggacc ttctcagcag agcccaagag
     6121 ctcatggagg ccgaggtcta cccgctcgaa gcgagcgcgc gccagtcatt cgaagaggtc
     6181 cttccggcgg caaacgaagc acgcgagcga tgcaaggctg cggggctgtg ggcgccgcaa
     6241 atgccgaccg agtacggagg catgggactc gacttcctgc cgcacgcgca catgagcgag
     6301 ctgctcggca ggagcccact cggccactac atattcggct gtcaggcccc ggacgcaggc
     6361 aacatggaga tcctgcacaa gttcggcacc gacgagcaga aagagaactg gctacgcccg
     6421 ctggctgccg ggaagattcg gagctgtttc tcgatgacgg aacccgatcg cgcggggtcc
     6481 aatcccacct ggatggagac gaccgcggtt cgcgacggcg acgatctcgt catcaacggg
     6541 cgcaagtggt tcacgaccgg aggacatctg gccgatttcg caatcgtcat ggccgtgacc
     6601 aacccggacg ctgcgccaca catgcgagcg agccagatca tcgttcccac cagcaatcct
     6661 gggttccacc acattcggcg cctctcgatc atgggcgacg tgggctacgg ctggcaaagt
     6721 cactccgagg ttgcgtacga agattgccgg gtgcccgcga acaatctgct cggtcaagaa
     6781 ggcttcgggt tcctgatcgc gcaggagcgg ctcggacctg ggcgcattca ccactgcatg
     6841 cgctggctgg ggatctgcga acgctccttc gatttgatgt gtgaacgcgc cgcccggcga
     6901 atggttgcac ccaaccggcc gctcgggtct cagcaggccg tccagcactg gatcgctgaa
     6961 agccgcgccg aaatcgactc tgcccgcctg atggtctacc aagcggcctg gaagatcgat
     7021 cgcgagggga cccgggccgc ccgcaacgag atatcggcca tcaagttcat ggtggcaggc
     7081 gttttgcaaa aagtcgtgga ccgggcgatt caagttcacg ggggtctcgg catgaccgac
     7141 gacactccgc tggcatggtt ctttcggcac gaacgggcgg cgcggatcta cgacggaccc
     7201 gatgaggttc ataagtccgc tctcgctcgc tcgattctca agaagtacgg cattgagcta
     7261 gggtcacaag ggagctaggt ttcatcgcgt gaggtgggca cggtatttgc gtttgagctt
     7321 cgggggcttt ctgatcgccg gagcgcttct atgcgcggtg caagtcacgt tcgggcagaa
     7381 gcccgacaaa gaagcggatg attctggtta caggcctgcc ccgagcgcca acgttgaagg
     7441 cgatgtgcag gccctgccga tcgacgcacc cgctggcagc gaggtcgtcg tcgttgcgat
     7501 cgaccggacg gtcgagctag gactggctgc gttcgtgcgg cgcgcgatcg agacgaatcc
     7561 cgatgccgca gcgatcgtcc tcgaggtgaa cacgctcggc ggccgcgtcg acgcggccat
     7621 ccagattcgc gatgctctgc tcgaagccaa gccgcgtacc gtcgcgttca tccaccctcg
     7681 tgcgatcagc gccggtgctt tgatcagttt ggcgtgtgac aacatcatca tgaccgacgg
     7741 cgctaccatt ggcgccgcga cgcccatcca agccggccca ggcggtcaag ccgaggcggt
     7801 cggcgaaaag atggtctcgt acatgcgtgc cgagatgcgc acgacggcgg aggccaacgg
     7861 ccggcgtggc gacatcgccg aggccatggt cgacagcgaa gtcgtgatcg acggcatcgt
     7921 ggaagcgggc aagctactga ccttggacac cgaccaggcg ctcaagatgg gcatggcgga
     7981 tgcaaaggcg accgatctcg cggacgtgat gaaaatcctc acgctggaaa gcccagagat
     8041 catcgaagtc gagctcaact ggggcgaaga gatcgcgcgc tggctcaccg agcccacggt
     8101 gagcggcctg ctgctctcgg tcgggatgct cggattgatg gtggcctttt acacgcggtc
     8161 cgtcggagcc ttcaccctcg ttggctttgt gtcgctcgcg ctgttcttcg gcggccacgc
     8221 cgtcgtcaat ctcgtcggat gggaagaagc gctcttgttc ctatcaggtc tcgtgctggt
     8281 cgtcgtcgag atctttttcg tgcctggcct cggtgtgccg ggagtgctcg gattgatctt
     8341 cgtcattgct gcgctggttc ttgccctgat cggcattccg atcgacgtat cgttcaaaac
     8401 cggcgtgctc gccgatgcca tgactcgcgt cttgctctca ttgctcggcg cattcgttct
     8461 cgcgctcgtg gccatgcgct tgctctcacg aaccatggcg ggcaaaggct tcatcttgga
     8521 gaatgcagag acgggatttc tctcggcgcc aagcgcatcc aacctggtcg gacaggtcgg
     8581 cgaggcgttg accgacctgc gacccgccgg aaagatcgtg gtcggcggga agcgccacga
     8641 agctacgagc gagcgtgagt tcgttgcgcg cggcgcgcgg gtccaggtga tcggcaaaga
     8701 tggaccaacc ctcatcgttc gccccgagag ggaagcgtga ctccggtcat catcgtcgtg
     8761 gtcctcgtgg ttggcctact cttgctcttc acggaagttg ccgtgatacc cggcttcggc
     8821 gtggcaggag ccctcggcct gctcgcactc ggggcaggcg cggtcgccgc gtggacggag
     8881 ctcggaccct tctggggcgg catctccacg ggtttttcgt tcgtagccgc gggcttcatg
     8941 ctgttctggc tgcccaaaag ccgaatggga cgaatgatgg tcctcgagca cagccaggcc
     9001 gacgccgtgt cccagcggga tcagagcgag ctgctcggac gccgggggat caccgtgaca
     9061 ccgctccgtc cgatcggccg cgtccgtttc gggtcgagcg aagtcgatgt ggaatccgaa
     9121 ggtgaataca tcgcgtctaa tctcgaagtc gaagtcataa ccgtggaagg ctcacgcgtc
     9181 gtcgtgcgca tcccggatgc aaactaggaa ggtaatctca tgcagttcgc tccactcgcc
     9241 ctgatctttc tcctcttcat cgtcatcttc gtcttgttcg tctggctgat cccggtacgc
     9301 ctgtggatcg cagcctggtc gagcggtgcg cgggtcggct ttggcgagct gatcggaatg
     9361 cgccttcgtc gtgtttcacc gggcgccgtc gtcaatcctc gaatcgcagc ggtgaaggct
     9421 gggatcgaca ccgagaccaa cattctggaa tctcactacc tggcgggcgg caacgttcaa
     9481 cgtgtcgtca atgctctgat cagcgccgac aaagcaggca tctccctcgg cttttcgcaa
     9541 gccgcagcga tcgatctggc cggccgcgat gtgctcgagg ccgtacagac cagcgtcaat
     9601 ccccgggtga tcacgacccc gaagatcacc gccgttgcaa aggacggtat ccagcttctc
     9661 gtcattacgc gaatcacggt tcgctccaat atcgaccgcc tcgtcggtgg cgcaacggag
     9721 gaaacggtgg tcgcgcgcgt cggtgaaggc atcgtatcca cgatcggttc gtccttgaac
     9781 tacaaggagg tcctcgagaa ccccgacagc atctcgaaga acgtgcttgc gcgcggattg
     9841 gacagcggca cggccttcaa catcctttcg atcgacatcg cagacatcga tgtcggcacc
     9901 aacatcggtg cgaagctgat gaccgagcag gcagaagccg acaaacaggt tgcgcaggcg
     9961 cgcgccgaag gacgacgcgc gctggctgta gcagctgagc aagagatgcg cgccaaggtc
    10021 gaggagatgc gggctcacct ggtcgaggcc gaggcgaaga tccccgaggc gatggccgac
    10081 gcgctccggg ccggcaatct cggagtcatg gactactaca acctgcgcaa cgtttcggcg
    10141 gacaccgaga tgcgtgattc gatcagcaag tctacacgcg gctcgaacga tgacggcgaa
    10201 atctaggcct tttgcgatca aagcattgct ggcgcagccg aggaccttcg tggcgcggct
    10261 gatcgccagt gatctcatcg cgaagcgcgg ggaaggcccg ctcgaacgca aggtgctggt
    10321 ctacaaacgg cgaaggcgct gacggcgatg gaggagttgc tcagggaaca tctcggcgaa
    10381 atcgcgctcc tgatctacat cctctaccca ctgctcaagc gatggtggga tcggcagaag
    10441 aagaagcgcg agcagcgtgc cggcacgcct gagccaacgg ggcagacccc aacacggggc
    10501 gaagccccgc ggccgcagac tccagtcccc catcgaccgc cttcgaaacc ccgaatccaa
    10561 ccgtcggtcg caaagcagtc gtccgaggcc gatctcctgg aagccgcgcg cgctgagcgc
    10621 cttcgcctcg agcaagcgag ctcagcgttg ctggcgcgcg ctgaaggcga gccgcgactg
    10681 gccagactcg ttccggcggt gcgcgatgat ctgctcgctc ggcttcgcgt ggtcgagcgg
    10741 gcgctgagcg cgcggccgac ggtgtcgagc gtcgtccatg agaccaccgt gatgcgcggc
    10801 ctcgaatcgt tgcttcggca tctgaagatg atggcacaac agcggatgct gggccggtcc
    10861 gcgtttctct ccgaagccga cgccgtcgca gacgcttgct acgcgcctct catcgagttc
    10921 gcgggggcgc aggggctcga tctcaagacc agtcagccgc taatcgtcac cggcgactgg
    10981 gatctttcca tcgtacccag cttcgcgtcg actcgcctgg cccctctccg cttgccgagg
    11041 gccttcgagc agagcctttg gggctggccg gcgatcgcgc atgaggtcgc ccacgacttc
    11101 tactacagcc tcaaaggcct cgagcccagc ttgcgcgcac ggctcgggct gccgagcaag
    11161 gtcgagatgc cgatgtcctc ggcggagctc gatggcgcct ggctgcggaa cctatttggc
    11221 gcctggcttc ccgaggtcgt ggccgacacg ctcggcacca cgagcctcgg ccctgcctac
    11281 gtggaaacga tgctccgcgc cttccgtaat ccaagctcgc ctcagcgaac cgccgcaata
    11341 ctccaagatc gaagcctgat cgacgagcac ccgcctccgc ggctccgcct ctacatggcg
    11401 acccgcgtcc tacatcacct cggacggcac agtgaggcgg acgcactctg ggcacgatgg
    11461 gaggcggacc acgccgaagt tggtttctac ttcttgcccc tcgctggaca atgggttggt
    11521 ctttcggacg aagcactaca ctcggttgcg gattcgatca tcgacgccct ggtacaacgc
    11581 gcctggcccg agctcgaagg cttccacctg ctgaatattc ccggcttcgc gtatcagcac
    11641 gcggagcacg cgaaagtgac ggagctcagc gccgacctgc tgcacggagc ccagctcgat
    11701 gccgacgttc gatggatcat cgcggccgca gtcttggcgg cttccgcaca acctgcgctt
    11761 cacgatccca tcctccgctt agcgaggctt tcgattgccg gacgggccca acccgcggtc
    11821 gcattcgtcg ccccgaagcg tcgccacacc tcggcaacga tcggtgaaac cctggttgca
    11881 tcattgagac gaccagaggc catcaaagag gcaatcatcc tgggcgccgc gatcaggccc
    11941 tacaaacgac cccgctggca ttgacactgt cgatccggct gccccggtgc tagacgtacg
    12001 caatgcccct gaccggcttt caccccgcgg tggccacctg gttcgaacga acgttcggcg
    12061 aaccaacgcc cccgcagctg gaaggttggc cggctatccg cgacggacaa cacaccttga
    12121 tcgcatcgcc cactggatca gggaaaacgc tggc
//
LOCUS       DASRZF010000160        21211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2858926, whole genome shotgun sequence.
ACCESSION   DASRZF010000160 DASRZF010000000
VERSION     DASRZF010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 21211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21211
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2858926"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="VFG22_07195"
     CDS             <1..662
                     /locus_tag="VFG22_07195"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET6416065.1"
                     /translation="KQIFAREAPLEFDGVRYQIPYHGEGSTGQGKPLRSMIRARADIP
                     VYTGSMAPKSQAMAAEIADGCLLTCMHPERPEVLMKNFNEGFEKAGNGKGLESFDIAP
                     TVVVVIGEPERVMIPCKMQLALYIGGMGSKTKNFYKDYLSSVGFEEACEKVQSLFMQG
                     KRNEAIAAVPDEMVDALYLVGSKDRIRDRFQRWKDSPIGTMMVGTQDMDTLRFMAELN
                     A"
     gene            670..1326
                     /gene="npdG"
                     /locus_tag="VFG22_07200"
     CDS             670..1326
                     /gene="npdG"
                     /locus_tag="VFG22_07200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HET6416066.1"
                     /translation="MKIGIVGGTGREGRGLAVRWAKAGHDVFIGSRQAEKGAAKAAEL
                     SKEFGVGIRGADNVAACEYAELIIVTVPYGAHRPTFESIKDAVGDKVVVDITVPLQPP
                     KVRSVNLPKGESAALEARALLGEGTRLVAALHHISSEHLSDPEHAFDCDVLVCGDDKD
                     ARASVISLVSDLGLRGVDAGVLKNAIALESLTPVLLHINRHYKSVGSGIRITGISETE
                     "
     gene            1323..2138
                     /gene="cofE"
                     /locus_tag="VFG22_07205"
     CDS             1323..2138
                     /gene="cofE"
                     /locus_tag="VFG22_07205"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HET6416067.1"
                     /translation="MSPGEPPSSGLSVGSELLVRAIRGVPTIRPGDDLAEIIASSLAR
                     AGVELADGDVVVLASKIVSRAEGCFVDLSSISPTEDALRLGAEVDKDPRLVELILRES
                     VEVSRKTRGALIVRHRLGFISANAGIDASNAAPIDPPDGTGPWVLVLPRDPDASARRL
                     LTALGERFSVDIAVMITDSWGRPFRRGTVGFALGIAGLPPVWDRRGSTDRHGRMLEAT
                     ETAVADSIAAAADLVAGQADEGRPVTIVRGLHFQRSEESSEALLRNPDDDLYA"
     gene            2135..3070
                     /gene="cofD"
                     /locus_tag="VFG22_07210"
     CDS             2135..3070
                     /gene="cofD"
                     /locus_tag="VFG22_07210"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HET6416068.1"
                     /translation="MSDSLGDQLRVVALAGGVGGARLVDGLDHALPPGSLTVVVNTGD
                     DFRHWGLSVSPDLDTVVYTLAGLSPEDRGWGIDGDTFHVLAEAQRRGVDDWFQLGDRD
                     LVTHLTRTGAMARGDSLTDVTAALCKTLGVNRPILPMSDAPRPTTIVTRDGAELSFQH
                     WLVKERAMPAVQAVRSEGSELATKPVLDALSRADLVLICPSNPYVSIDPILGLRGVED
                     TLRNTRTVAVSPIIGGKAVKGPLAKMIREIEDRPPSAEAVAAHYEGLLNGYAAHSGDG
                     FAAPFPVLETNILIQSKEDRVRFAIELLKFSRSLS"
     gene            3067..3666
                     /gene="cofC"
                     /locus_tag="VFG22_07215"
     CDS             3067..3666
                     /gene="cofC"
                     /locus_tag="VFG22_07215"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HET6416069.1"
                     /translation="MSRWALIPIKGFERGKSRLSEVLAAPERARLARELFEHVVRVLR
                     ESAVIDEIAVVSDSMEARQHAERLGVIALADAADTGGLADVVDEALRDLSRRGASSVM
                     ICMGDLPNLTVQDIASVARLLDESDVVLVPDLSQQGTNVIALKSPTILPSCLGHEDSL
                     LRHKAQARQLGLTVGIQLSSGIGFDVDRPSDLARLRRRY"
     gene            complement(3660..4469)
                     /locus_tag="VFG22_07220"
     CDS             complement(3660..4469)
                     /locus_tag="VFG22_07220"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6416070.1"
                     /translation="MSRFAERYGPWAVITGASAGIGAEFATRVAELGVNVVLVARRRE
                     RLEEIASNIEDMYRVEARVAPADLSARDIVEVLRPVIADIEIGLLINCAGFGSSGPFL
                     DMDPGVQEAMLNLNCRAPLLLTHEIAQGMRERGRGGVIIVSSVNAFSAASSMANYNAT
                     KSYDLLLAEGLAGELRPYGVDVQALCPGGTLTEFQQVAGLDTRKFGPFASLVFMSPTS
                     VVTTSLRTLGRRVTVVPGLLNKLTVVSMKLLPRRLAAWLLGAIMRRFSASQ"
     gene            complement(4510..5787)
                     /gene="cofH"
                     /locus_tag="VFG22_07225"
     CDS             complement(4510..5787)
                     /gene="cofH"
                     /locus_tag="VFG22_07225"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: protein motif:HMM:NF005609.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HET6416071.1"
                     /translation="MTTAIETCLERVSPDVRLILERSLAGHEVSVEDGIALNEARGTD
                     LHALCLVADELRRAQVGDRVTYVVNRNINFTNVCIKNCKFCAFARTVRSEQGYFLPHE
                     EVARRVRQAWEMGATEVCLQAGLSPNLTGDSYIELCRVVKEAAPKVHIHAFSPEEVKF
                     GASLKRVSYGDYLAELKEAGLGTLPGTSAEILDDRLRKTISPTRITTSEWLDVITAAH
                     RLGIRTTSTMMFGHLESVEDRMRHMDLLRRVQRETGGFTEFVPLSFVHEEAPLFATSG
                     VPGVRPGPTGDEVLRLYATARLVLGRDIPNLQASWVKEGLRTAQLLLSCGVNDLGGTL
                     MNESISTAAGARNGQLMTPATLRRVIRDAGRVPAERGTDYRIIREFGGTSDQDPSEPL
                     DTVADPDSVFGSYDALTADPRFHYEPRGLRVVS"
     gene            complement(5784..6926)
                     /gene="cofG"
                     /locus_tag="VFG22_07230"
     CDS             complement(5784..6926)
                     /gene="cofG"
                     /locus_tag="VFG22_07230"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF004884.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase CofG"
                     /protein_id="HET6416072.1"
                     /translation="MSSVSSILERASSEPVSDADAYALSALGTAPLCEAARARRDRAW
                     GRTLTFSPKVFLPITNLCRNRCDYCSFRRSPGDPGEWTMSPDEVRTWLHRARKQGCIE
                     ALFCLGDTPETGFREYHALLRSWGHASTVEYLHWAAEEALGAGLLPHTNAGILSREEM
                     IRLRPVNVSLGLMLENVSERLCGKGMPHQRAPDKRPARRIQMTREAGELRIPFTSGLL
                     IGIGESVEERVDTILAIRSLQREHGHIGEVIVQNFRSHPDTPMGFATEPTPDELAATI
                     ALARLILDDEVSVQAPPNLNPASTAALIQAGINDFGGISPVSPDYINPRHPWPFLDQL
                     RTACQGEGFRLEPRLPLYPTHLDKPGFVDATLRPRVDQVHRELASP"
     gene            complement(6923..7798)
                     /locus_tag="VFG22_07235"
     CDS             complement(6923..7798)
                     /locus_tag="VFG22_07235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HET6416073.1"
                     /translation="MKFSASIAMAPPEHYIPVAQEAEKLGYHAIVLPDSIFFSEEVSK
                     PYPYTQDGSRMWDGETPWIEPIVGAAAMAAATSSIYFYTSVVKLPVRHPVLVAKQVSS
                     LAALSNNRFGFGCGLGWLPEEFKWCGTEFETRGRRMDECLEILRLIWGGGMVEYHGEH
                     YDFGKIQMSPVPSEPIPIYIGGHTEPGLRRAAKFGDGWSSAMLTSKKLLDLIGRIEAY
                     RKEYGRDHLPMEYQAVVTDVWDADGYKRLEDAGVTDIITVPWLMYGTPMVGGELQGRI
                     DGLNRFADTVIAKMQ"
     gene            complement(7821..9026)
                     /locus_tag="VFG22_07240"
     CDS             complement(7821..9026)
                     /locus_tag="VFG22_07240"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET6416074.1"
                     /translation="MTTKSHRETDPRGVGLTTQDVEAIWDSVVTFYKAGLHPAIALCI
                     RRRGEIILDRAIGHASGNSPQDPEGTPLVKATPDTLYNFFSGSKSVTAMLIHLLQEEE
                     QLHVDEPVATFIPEFAKRRKHSITIRDVLTHRAGIPATPEEAINLDLMSHPEEIVRAF
                     CELEPTHRPGSVQAYQAVTSGFILGEIIRRVTGKDIRGFLTARVREPLKMKHFNYGVP
                     PEMLDRVAVDAFTGPTPTWPAKDLVQVAFGASMQELVDLGNDPRFRTGVAPAANLIAT
                     PEEISRFFETLLCGGTYEGTRVFDQRTVARAIEPQVTGQVDRVLMMPIPLSMGFMLGG
                     RTFGFYGPRTATAFGHLGFTNVLGWADPDRDISVGFMNTGKPLLSARMLAWLNVTRVI
                     GTRIPRDRA"
     gene            complement(9089..12760)
                     /gene="metH"
                     /locus_tag="VFG22_07245"
     CDS             complement(9089..12760)
                     /gene="metH"
                     /locus_tag="VFG22_07245"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HET6416075.1"
                     /translation="MSDDATTERLKQLIEQRIVFFDGAMGTMIQRAGLGESDFRGDRF
                     RDHPSSLQGNNDLLVLTRPDVIRGIHEAYLDAGADIIETNTFNANAFSQADYDLQEVV
                     YDLNVAAARLAKEATASRSDKPCFVAGAIGPLNKTLSLSPDVNDPGYRALTFDQARKA
                     YAEQVRGLIDGGSDLLLVETIFDTLNAKAALCAIDDVFGEKGVRLPIVISVTIVDKSG
                     RTLSGQTVEAFYISIEHAEPLAVGINCSLGAQEMRPFLKELAGIADCYVHCYPNAGLP
                     NAFGGYDQTPEEMAGLLRGFAADGMINIIGGCCGTTPDHIRAISEAVRDVAPRPKPHR
                     ETHYPRFSGLEPLIIRPETNFMMVGERTNVTGSARFRKLITQGDYPEAVSVALQQVRS
                     GANIIDINMDEGMLDSEQAMTTFLNLIGAEPEISRVPIMLDSSKWSVLQAGLKCVQGK
                     SIVNSISMKEGEPEFLERAREVRRYGAAAVVMAFDEEGQADTIERKVEICSRAYRILV
                     EEVGFAPTDIIFDPNIFAIATGIEEHNDYAINFLEATKQIKKRCPGALISGGVSNLSF
                     SFRGNDRIREAMHSAFLYHAIASGMDMGIVNAGQLEVYEEIPKELLEHVEDVLFNRRA
                     DATERLVTLAETVKGKGKTQVHDLSWRDAPVEERLSHALVKGVVEFVVEDTEEARLKY
                     PRPLDVIEGPLMSGMAVVGDLFGAGKMFLPQVVKSARVMKKAVAHLEPFMEEEKADGS
                     AQGKVLMATVKGDVHDIGKNIVGVVLGCNNYAVVDLGVMVPADKILDTAIAEGCDIVG
                     LSGLITPSLDEMVNVASEMQRRGMKLPLLIGGATTSRAHTAVKIAPAYEGSTMHVLDA
                     SRVVNAVSGLLSPERRAEFDAKNREEQEKSRKLFEHRQNRELVSIEVARENHVPIDWA
                     SQGLPTPAFRGRRIIDEVSLEEIAEYIDWTFFFSAWELRGKFPKILEHESYGPAAREL
                     YQHGRELLDRIIAEKLLTPRGVYGFWPANQDGDDIVVWHDEAREKERLRFHMLRQQGV
                     KPNEQPYFSLSDFVAPIDSGIADYIGAFAVTTGIGAEALSRAFEQDHDDYNAIMVKAL
                     ADRLAEAFAELMHQRARREWGYEPENGFSKEELIAEKYRGIRPAFGYPACPDHTEKKT
                     LWKLLGADEIGISLTESFAMNPGASVSGIYLGHPSSRYFAVGPIDRDQVEDYARRKSM
                     PLEEAERWLQPNLGYQRGG"
     gene            12898..13953
                     /locus_tag="VFG22_07250"
     CDS             12898..13953
                     /locus_tag="VFG22_07250"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET6416076.1"
                     /translation="MNSNRLAFGTAWVRFLLAACGIWLVPLIYPPMSAHRWVFVAYAG
                     VALVEQFLIWRDIAGPWRPVLGGILDMALLTFIVQRIGSSRTAIVALYFVAGIVNSLV
                     VTPRLGVLLSSIGAAMYSSVVMMEVRGVLPFAPDGPLWLQAKPPTALEGFMQAFLVTI
                     LLMFGTAIVATLIQKINQRESQLLVANERLAELSRQDPLTKLWNRRHILSHIEQGLAW
                     LNRGRPMAVVMIDLDQFKRVNDDRGHLEGDQVLQDVADALRRSTREVDIAGRYGGDEF
                     VIVLPDTNQEEGRVAGNRFVTAIREVGMKFDPASPVTASAGLSIARPGDTVEAVLKRA
                     DDFAYSAKGKGGNRLCG"
     gene            13957..14808
                     /locus_tag="VFG22_07255"
     CDS             13957..14808
                     /locus_tag="VFG22_07255"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6416077.1"
                     /translation="MSDKVLIAGCGDVGNALAKRLLAEGCDVWGVRRRVEALADGVQP
                     WRIDLMNDPRSFGAPPAAFDYLFYTASADRREEDAYRAIYVDGLRNLWTALRDARCKL
                     RRVLFTSSTAVYGQSKGEWVDENSATEPQGFNGRILLEAEAVVRETPEEGISLRLSGI
                     YGPGRTRLVRKVWSGQATATGSWTNRIHVDDCAGALHHLMRVENPHSLYLGSDDRPAT
                     TAEVVTWLSNELRVPTPPNAEAEQLNKRCRTSRLRESGYRFAYPTYREGYGPIVREFL
                     SQTGVDG"
     gene            complement(14813..15133)
                     /locus_tag="VFG22_07260"
     CDS             complement(14813..15133)
                     /locus_tag="VFG22_07260"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET6416078.1"
                     /translation="MADQINASHILLMYAGSQRSSATRSKENAQSQIADIEQQLRVGA
                     DFAALAREHSDCGSAAAGGDLGAFGRGQMVKGFEDAAFALDVGGTSGVVETPFGFHII
                     RRTA"
     gene            complement(15189..16574)
                     /locus_tag="VFG22_07265"
     CDS             complement(15189..16574)
                     /locus_tag="VFG22_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HET6416079.1"
                     /translation="MDELSLLAAANEAPHRDCLVVNGTIWSYGDLADRVCAALSALIA
                     RGVAPGDRVALTPRVDLDSIVSLYALFELGCPAVLLHPRLSSREQSLVLEEAEPVHVI
                     AEPLPRDLQTVAPQRSESIASDSTLAIVYTSGSKGRPRGARLSRRAFIASASAHAENL
                     DWRPDDRWLLTMPLAHVGGLSIVTRSLIARRCVVLGPTAFDPREVAQIMETQDVTLLS
                     VVPTMLRRLLQLAQPEWKPGTGLRAVLVGGAPFPDALRELATERKVPTLATYGCTEAC
                     SQISTQTPMQTGQPGSGRPVPGVDVRIESGEIQVRGDVLMDGYLGEDRSAEPWTRDGW
                     LRTGDFGSFLPDGQLLVLGRIDDLIVTGGENVAPAEVEAWLETVPGIAMACVFSQPHE
                     EWGEEVCAAIVIEGAEYSGDALRDRMTNELATHKRPRRIALLNALPLNRTGKVDRAAA
                     RSLSVSKLRPI"
     gene            complement(16567..17640)
                     /locus_tag="VFG22_07270"
     CDS             complement(16567..17640)
                     /locus_tag="VFG22_07270"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HET6416080.1"
                     /translation="MTELHAQIDVLEGKLSQSLVGAATGLEERTFAVLRLMRSDGTSG
                     QGEASPLPGYSLDSIDEVASELQRLAEGPIRVNPLAGPLELLTETFAAHPMAHPASRL
                     AFETALLDWLGQTRGEPLHRVLAGDAERQRIPIADLVMDPDPGSWPARADALIADGAT
                     HLKLKIGPDLETELRALRTIRETHPSVALRLDGNQRVSLSALRKHSAVLESLGLELIE
                     EPVTPGDWIAALSLPLPFALDETLRDPDLTERLLDTGKIRAVVIKPMVIGGLRAAFAA
                     AEHAACHAVPYLVSHSFDGPIGRAVTAELALALQTELAAGLGAHPALALWPPHRIAAI
                     EGREIVPHDEPGLGLQFEELADG"
     gene            complement(17637..18527)
                     /locus_tag="VFG22_07275"
     CDS             complement(17637..18527)
                     /locus_tag="VFG22_07275"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: protein motif:HMM:TIGR00751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HET6416081.1"
                     /translation="MANPAPGSVGAWFLAARPATLTAAFAPVAVGTACAWSVNRFRWD
                     AALAALLGAFLIQIATNFANDMFDFQKGADDEERLGPTRTAQAGLLTTGQLKQGIILA
                     FALALGTGIYLTWLAGPVVILIGLASMAAGLAYTGGPFPLAYNGLGDVFVMAFFGFVA
                     VAGTAFVQASFVPEVAWLASLPIGSLATAILVVNNVRDFEGDARAGKGTLVVRFGRSR
                     GVLEYGFLLLLSYATPMVLLGLGWTSAWVLLPLLTIPIAARLYHSVVADRGVALNRTL
                     SGTAKLLSIFGVLFAIGIAL"
     gene            complement(18527..19273)
                     /locus_tag="VFG22_07280"
     CDS             complement(18527..19273)
                     /locus_tag="VFG22_07280"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6416082.1"
                     /translation="MWTLIHGFAGSPRSWTHLVELARFERPPLLATLAGHGPNWRHRK
                     VDSFERELERLASEIESTEQPRLVCGYSMGARIALGLLARRPRLFDAAVLVGVHPGLD
                     SQSDRGARREADSAYSRLLRDQGLAAFVDAWEDVPLFSSQRTLSAETLAEQRAIRLDH
                     EAEGLALALELLGLAEMPDYRALMRSYETPITLVTGALDSKFSAIAETLRAANPTIGV
                     ERIPGVGHNVVLEAPDALAELLNRVERQVA"
     gene            complement(19273..21057)
                     /gene="menD"
                     /locus_tag="VFG22_07285"
     CDS             complement(19273..21057)
                     /gene="menD"
                     /locus_tag="VFG22_07285"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HET6416083.1"
                     /translation="MATVAELQTEWARLVIDSFVQAGVGDAIISPGSRSTPFVIAALE
                     HEGLECTSAIDERSAAHYALGQARGALRPSLLVCTSGSAPANYFPAVVEASEAGVPLI
                     VLSADRPPELHGCGANQTTDQVGLFGTKVRFFANLGEPRDDALGLRAARRIIAQAVSE
                     ALGAKPGPVHINAQARKPLEPVPVASELHARVDAVLAEPLTQLHRARPLDPKLVELVP
                     SLVTDSVAEALDSAARPVILCGPADLRSAMFVDALDELAERSGAVVLAETSSQFRCWA
                     KSRALGGFDTIWQTANGRSLCRPDFVLQLGATPVSVGWERICAEERIRRIVVHPWKWA
                     DPSSDAEAVVQADIGSFLVGLCEAEYHLNRRHPEFLPGFEWAESAVWDEVSAILEQSG
                     DALSQAGAARALLDALPSPSALVLGNSLAIRDADIWAPPSDKLLLVHSQRGVSGIDGV
                     VSGAAGVASVVEGPTTLLIGDVSFLHDINGLDLASRLDGPLVIVVVNNAGGQIFQQLP
                     IAQQGKESWLSYFTTPHGADLESAARVYGCEFESANTVSGFRRALDSAYGRAGCTVVE
                     ALVPTQSAREQSRELIRRVERALRSESS"
BASE COUNT         4080 a         6611 c         6551 g         3969 t
ORIGIN      
        1 tgaagcagat tttcgcgcgc gaggctccgc tcgagttcga cggtgttcga tatcagatcc
       61 cctaccacgg cgaaggatcg acggggcaag gcaaaccgct tcgaagcatg attcgcgccc
      121 gcgccgacat acccgtctac accggatcga tggcaccgaa atctcaggcg atggccgccg
      181 agatcgcaga tggctgtttg ctgacctgca tgcacccaga gcgcccggag gttctcatga
      241 agaacttcaa cgaaggcttc gagaaggctg gcaacggaaa gggactcgag agcttcgaca
      301 tcgcccctac cgtcgtcgtg gtcatcggcg agccggaacg ggtgatgatt ccttgcaaga
      361 tgcagctcgc gctctacatc ggtgggatgg gctcgaaaac gaagaacttc tacaaggact
      421 acctcagctc cgtcggtttc gaagaagctt gcgaaaaggt tcaatctctg ttcatgcagg
      481 gaaagcgcaa cgaggcgatc gccgccgtgc ccgatgagat ggtcgacgcc ctctacctgg
      541 taggctccaa ggatcgcatc cgtgatcgat tccagcgctg gaaagactcg ccgatcggta
      601 ccatgatggt cggtacgcag gacatggata cgcttcggtt catggccgag ctcaacgcgt
      661 gaggcaacga tgaagattgg aatcgtaggt gggacggggc gcgaaggtcg cggtttggca
      721 gtccgatggg ccaaggccgg gcacgatgta ttcatcggct cacgccaagc cgagaaggga
      781 gcagccaagg ctgccgagct gtcgaaggag ttcggagtcg gcattcgcgg cgccgataac
      841 gtcgccgcat gcgaatacgc cgagttgatc atcgtcacgg ttccatacgg agcgcatcga
      901 ccgacgttcg agtcgatcaa ggacgccgtg ggagacaagg tcgtcgtcga catcaccgta
      961 cccttgcagc cgccgaaagt gcgttcggta aatctgccca aaggcgaatc cgccgcgctc
     1021 gaggcccgcg ccctactcgg cgaaggcacg cggctcgtcg cagcgttgca ccacatcagc
     1081 tcggaacacc tcagtgaccc ggagcacgct ttcgattgtg acgttctggt ctgcggcgac
     1141 gacaaagacg cgcgtgcttc cgtgatttca ctggtttccg atctgggact gcgcggcgtg
     1201 gacgcgggtg ttctcaagaa cgcgattgcg ctcgagtccc tcacgccggt tctgctgcac
     1261 atcaatcgcc actacaagag cgttgggtcc ggcatacgaa tcacgggcat ctcggagacc
     1321 gagtgagccc tggcgagcca ccttcgagcg gcctaagcgt cggctcggag ctcctggttc
     1381 gcgccattcg aggtgtgccc acgatacgcc cgggtgacga cctcgccgaa attatcgctt
     1441 cgagtctcgc gcgtgctggg gtcgagctcg ctgatggcga tgtggtcgtt ctcgcgagca
     1501 agatcgtttc ccgcgcggaa ggctgcttcg tcgatttgag tagcatctcg ccgaccgagg
     1561 atgccctgcg actcggcgca gaagtcgaca aagatccgcg gctcgtggag ctcattctgc
     1621 gtgagagcgt cgaggtttcc cgaaagacac gaggtgcgct gatcgtacgt catcgcttgg
     1681 gtttcatttc ggccaacgcg ggtatcgacg cgagcaatgc agcgccaatc gatccaccgg
     1741 atggcacggg cccctgggtc ctggttttgc ctagggatcc cgatgccagc gctcggagac
     1801 tcctcactgc actcggagag cgattctccg tcgacatcgc cgtgatgatc accgactcct
     1861 ggggccgccc attccgacgc ggcaccgtcg gctttgcgct cggcatcgcc ggtcttccgc
     1921 cagtctggga ccgtcgcggc tcgacggacc gacacggccg gatgctcgag gcaaccgaga
     1981 ccgccgtcgc cgattcgata gccgcagccg cggacctcgt ggcgggccaa gccgatgaag
     2041 gccggcccgt gaccatcgtt cgggggctcc actttcaacg gagtgaggag tcgtctgaag
     2101 cccttctgcg caacccagac gatgatctct acgcatgagt gactcgttag gagaccagct
     2161 gagggtcgtc gcgctcgccg gaggagtggg tggagcgcga ctcgtggatg gactcgacca
     2221 cgccctgccc ccgggttcac tgaccgtcgt cgtgaataca ggggatgact ttcgacattg
     2281 gggcctctcc gtgagccccg acctcgatac ggtcgtgtac accctggctg gcctctctcc
     2341 ggaggatcgt ggctggggca tcgacgggga cacgtttcac gtcttggctg aggctcaacg
     2401 ccgtggcgtc gacgattggt ttcaactcgg tgaccgggac ttggtcacac atctgacgag
     2461 gaccggcgcg atggcccgag gcgactcgct cacggacgtg accgccgcgc tttgcaagac
     2521 cttgggcgtc aatcggccga ttctcccgat gagcgacgcc ccgcgcccaa cgaccatcgt
     2581 gacgcgagac ggcgccgagc tcagctttca gcactggctg gtgaaggagc gagcgatgcc
     2641 agcggtccag gcggtccgct ccgaaggaag cgagctggcg accaagcccg ttctcgatgc
     2701 cttgagccgt gccgatctgg tcctgatttg cccttcaaat ccgtacgttt cgatcgatcc
     2761 gattctgggc ttgcggggcg tcgaggatac acttcgaaac acgagaaccg tggcggtcag
     2821 cccaatcatc ggtgggaaag ccgtgaaggg cccgctggcc aagatgatcc gagagatcga
     2881 agaccgtccc ccgtctgccg aagcggtcgc cgcacactac gaaggcctcc tgaacggata
     2941 cgccgcccat tcaggggatg gtttcgcagc accctttcca gtactggaga cgaacatcct
     3001 gatccaatcg aaggaagatc gggtacgctt tgccatcgag ctgctcaaat tttcccggag
     3061 cctctcatga gtcgttgggc gctaattcca atcaagggtt tcgagcgggg caagtccagg
     3121 ctttccgagg tgctcgcggc accggaacga gcgcgcttgg ctcgcgagct cttcgagcac
     3181 gtcgtgcgtg tcctcagaga gtccgccgtc atcgacgaga tcgccgtcgt cagcgactcg
     3241 atggaagcac ggcaacatgc cgagcgactc ggtgtcattg cgctcgccga cgcagctgat
     3301 accggcgggc tcgccgatgt agtcgacgag gcactccgag acctcagccg ccgtggagcg
     3361 agcagcgtga tgatctgcat gggcgatctc ccaaacttga cggtgcaaga catcgccagc
     3421 gttgcgcggc tgctcgacga aagcgacgtc gttctcgtgc cggacctctc gcagcaaggc
     3481 accaacgtca ttgccttgaa atcaccgacg atcctcccta gttgcctggg gcatgaagac
     3541 agcctgctcc gtcacaaagc gcaggcgcgc cagctcggcc tcacggtcgg cattcagctc
     3601 agcagcggga tcggcttcga cgtcgaccgc ccgagtgacc tcgcccgctt gagacgccgc
     3661 tactgactcg ccgagaagcg acgcatgatg gcccccaaaa gccaggcggc taggcgccta
     3721 ggcaagagct tcatagacac gacggtgagc ttgttcagga gtcccggaac gacggtcact
     3781 cgccggccga gtgttcgcag gctggtcgtt acgaccgagg tcgggctcat gaagaccagc
     3841 gacgcgaacg gaccaaactt ccgcgtatcg agacctgcga cctgctggaa ctccgtaagc
     3901 gtgcctcctg gacacagcgc ctggacatcg acaccgtagg gacgcagctc accggcaagt
     3961 ccttcggcga gcaataggtc atatgacttg gtcgcgttgt agttggccat gctgctcgca
     4021 gccgaaaacg cattgaccga ggaaacgatg atcaccccgc cccgcccacg ctcgcgcatg
     4081 ccttgcgcta tctcgtgggt gagcaggagg ggtgctcggc aattcaaatt gagcattgcc
     4141 tcttgaactc caggatccat gtcgagaaat gggccactcg aaccgaaacc cgcgcagttg
     4201 atcaaaaggc cgatttcgat gtctgcgatg accggccgca agacctccac gatgtcccgc
     4261 gcagacagat ctgcgggcgc cacccgtgcc tctacgcgat acatgtcttc gatgttcgag
     4321 gcgatctctt cgagacgctc ccgccttctt gcaaccagga ccacgttcac gccgagctcc
     4381 gcgacgcgcg ttgcgaactc cgccccgatt cctgcggatg ctccggtgat gaccgcccag
     4441 ggtccatacc gctctgcgaa cctactcatc agccgccacc gtaccatgcg gcactggaac
     4501 gcacagcgac tagctaacga cccgaagacc tcgcggctcg tagtggaaac gcggatctgc
     4561 ggtcagggca tcgtacgagc caaagacgga gtcgggatct gccacggtat ccagcggttc
     4621 gctcgggtcc tgatcggatg taccgccaaa ctcgcgaatg atgcgatagt cggtgccccg
     4681 ctccgccggg actctcccgg catctcgaat cacgcgacgc aacgtcgccg gcgtcatgag
     4741 ttgcccgttg cgcgcaccgg cagcggtaga aatgctctcg ttcatcagag tgccgcccaa
     4801 atcgttcaca ccacagctca gcaggagttg cgcggtgcgc agcccctcct tgacccacga
     4861 tgcttggagg ttgggaatgt cccgacccag caccagacga gcggtcgcgt agagacgcaa
     4921 gacttcatcg cccgttggac caggacggac gcctggcaca ccgctcgtgg cgaacagagg
     4981 ggcctcttca tggacgaaag aaagagggac aaactcggtg aaaccgcccg tctctcgctg
     5041 aacgcggcga agcaggtcca tgtgccgcat acgatcctcg accgactcca ggtgcccaaa
     5101 catcatcgtc gacgtagtcc gaattccaag tcgatgcgcc gccgtgatca cgtcgagcca
     5161 ctcgctcgtg gtgattcgag ttggcgagat cgtcttgcgc aagcggtcgt cgaggatctc
     5221 cgcagaggtt ccaggaagcg tccccaatcc cgcctccttg agctccgcca agtagtcgcc
     5281 ataggaaact cgcttgaggg acgcgccgaa cttcacttct tctggcgaga atgcgtgaat
     5341 gtgaactttc ggcgcggctt ccttcacgac tcgacaaagc tcgatgtaac tatcgccggt
     5401 gagattcggt gagagcccag cttgcaggca gacctccgtg gcccccattt cccaggcctg
     5461 tcgtacgcgc cgggccacct cttcgtgtgg aagaaagtag ccctgctccg agcgtaccgt
     5521 gcgagcgaat gcgcagaact tgcagttctt gatgcaaacg ttcgtgaaat tgatgttgcg
     5581 gttgacgacg tacgtgacgc ggtcaccgac ctgcgcacgg cgcaactcgt cggccaccaa
     5641 gcaaagagcg tgcagatcgg tgccacgcgc ttcattcagt gcgatgccat cttccacgct
     5701 gacttcgtgg ccggccaggc ttcgctcgag gatgaggcga acgtccggag agactctttc
     5761 taggcaggtt tcgatggcag tcgtcatggc gaggcaagtt ccctgtgaac ttggtcgacg
     5821 cgggggcgca acgtcgcgtc gacgaagccc ggtttgtcga ggtgagtggg gtataacggg
     5881 agtcgcggtt cgagtcgaaa gccttcgccc tggcaagccg ttcgaagttg gtccaaaaac
     5941 ggccaggggt gcctcgggtt gatgtagtct gggctcaccg gggagatccc gccgaaatcg
     6001 ttgatgccag cctgaattag ggccgcagta gaggccggat tgaggttggg aggcgcctga
     6061 acgctgacct cgtcgtcgag aatgagtctt gccaaagcaa tggtcgccgc gagctcatcc
     6121 ggggtgggtt ctgtcgcaaa tcccatcggc gtatctgggt gggaccgaaa attctgaacg
     6181 atgacctcgc cgatgtgacc gtgctcacgc tgcaagctcc gtattgcgag gatcgtgtcg
     6241 accctttctt ctaccgattc gccgatgccg atcagcaagc ccgacgtaaa cgggatcctc
     6301 agctcgccgg cctcccgtgt catttggatg cgtcgggcgg gtcgcttatc cggcgcccgc
     6361 tgatggggca tccctttgcc gcagagtcgc tcgctcacgt tctcgagcat caaacccaag
     6421 ctcacgttga ccggcctcaa acgaatcatc tcttcgcgtg agaggatccc tgcatttgtg
     6481 tgtggaagca ggccagcgcc cagggcctct tctgccgccc agtgcagata ctcgaccgtg
     6541 ctcgcgtggc cccaggagcg aagcaacgcg tggtattcgc ggaacccggt ctcgggcgtg
     6601 tcgcccaagc agaagagcgc ctcgatacaa ccttgtttac gcgcgcgatg cagccacgtc
     6661 cgaacttcat cggggctcat cgtccactct cccggatctc ctggggaacg tcggaacgaa
     6721 cagtaatcac atcgattgcg acaaagattg gtgatcggga ggaacacctt cggcgagaac
     6781 gtgagagttc ggccccatgc tcggtctcgc cgcgcacgcg ccgcttcgca aagaggagcg
     6841 gtaccgagcg cgctcagagc gtaggcatcg gcatccgaca ccggctcgct gctcgcccgc
     6901 tccaaaatcg aagaaacgga gctcattgca tcttggcaat aacggtgtct gcgaaacgat
     6961 tcaagccatc gatacgcccc tgcaactctc ctcccaccat cggagtcccg tacatgagcc
     7021 agggaaccgt gatgatgtcg gtgacaccag cgtcttcgag tcgcttgtag ccatcggcat
     7081 cccaaacgtc agtcacgacc gcctggtact ccatcggcag atggtcgcgt ccgtactcct
     7141 tgcgatacgc ctcgattcga ccgatcaggt ccagaagctt cttcgatgtc agcatcgcgg
     7201 aagaccagcc atcgccaaac ttggctgcgc gccgaagccc cggctcggtg tgccctccga
     7261 tgtaaatcgg aatgggctcg ctcggcaccg ggctcatttg gatctttcca aaatcgtagt
     7321 gttctccgtg gtactcgacc attcccccgc cccagatcag gcgaagaatc tcgaggcact
     7381 cgtccatacg cctgccacgg gtctcgaact ccgttccaca ccacttgaac tcttcgggca
     7441 gccaaccgag accgcaacca aagccaaagc ggttgttgga gagcgcggcg agtgaggaga
     7501 cctgcttggc aaccaggacc ggatgacgca cgggcagctt caccacgctc gtgtagaagt
     7561 agatcgacga ggttgcggct gccatcgcgg ccgcaccaac gatcggctcg atccagggcg
     7621 tctcgccgtc ccacattcga ctgccgtcct gcgtatacgg atagggtttg ctgacctctt
     7681 ccgagaagaa aatcgagtcg ggcaagacga tggcgtgata gccgagcttc tcggcctcct
     7741 gagccaccgg gatgtagtgc tcgggtggcg ccatggcgat cgaagcgctg aatttcatgc
     7801 cgcagaccct agcacgcggc tcaggcacgg tcccgcggga tgcgggtgcc gatcacacgg
     7861 gtgacgttca gccaagcgag cattctggcc gacaagagcg gcttgcccgt gttcatgaat
     7921 ccaacgctga tgtcgcggtc tgggtcggcc cagccgagga cgttggtgaa gccgagatgc
     7981 ccgaaggcgg tagcggtccg cgggccgtag aagccaaacg tgcgccctcc gagcatgaac
     8041 cccatcgaca gggggatggg catcatcagg actcgatcga cttgcccggt gacctgaggt
     8101 tcgatggcgc gcgcaacggt tcgctgatcg aacacgcgtg tgccttcgta ggtgccgccg
     8161 caaagaagcg tctcgaagaa gcgcgatatc tcctcgggtg tggcgattag gttggctgcg
     8221 ggcgcgacac cggttcgaaa acggggatcg ttaccgaggt cgaccagctc ctgcatcgac
     8281 gccccaaagg ccacttgcac taggtcttta gccggccagg tgggcgtagg ccccgtgaat
     8341 gcatcgacgg cgacccggtc gagcatctcg ggcggcacgc catagttgaa gtgcttcatc
     8401 ttgagcggtt cccgaacccg agcggtcaag aagcctcgta tgtctttgcc ggtcacgcga
     8461 cggatgatct cacccagaat gaacccactc gtcaccgctt gatacgcttg tacgctcccc
     8521 ggtcgatgcg ttggctccag ctcgcaaaac gcgcgaacga tctcttcggg gtgggacatg
     8581 agatccagat tgatcgcctc ttcgggcgtc gccggaatgc cagccctgtg tgtcagaaca
     8641 tctcgaatgg tgatgctgtg cttccggcgt ttggcaaact cgggaatgaa cgttgcgacc
     8701 ggctcgtcta cgtgaagctg ctcctcctcc tgcagaaggt gaatcagcat cgcggtgacg
     8761 gatttcgagc cggaaaagaa gttgtacaag gtgtccggtg tggccttcac gagcggcgtg
     8821 ccctcggggt cctgcgggct gttcccactg gcgtggccga tcgctcgatc gagaatgatc
     8881 tcgcctcttc ggcgaatgca gagggcgatc gccgggtgaa gcccagcctt gtagaaagtc
     8941 acgaccgagt cccagatcgc ctccacgtcc tgagttgtga gcccgacgcc acgcggatcg
     9001 gtttcccgat gactcttcgt agtcacggca tcagagtcga agcggcgaat ggtgaccgga
     9061 agcaaatctc ggagccgggg cctccgcatc agccaccccg ctgatagccg aggttgggtt
     9121 gcagccagcg ctcggcctcc tcgagcggca tgctctttct tctcgcgtag tcttcaacct
     9181 gatcgcggtc gatgggtccg acggcaaagt accgagagct ggggtggccg aggtagatgc
     9241 cgctcacgct cgcaccgggg ttcatcgcga agctttcggt gagcgaaatg ccgatctcgt
     9301 cggcaccaag cagcttccag agcgtcttct tctccgtgtg gtccgggcaa gccggatacc
     9361 caaaggccgg cctgatgccg cggtacttct ctgcgatgag ctcttctttg ctgaagccgt
     9421 tctccggctc gtaaccccat tcacggcggg cacgctggtg catgagctcg gcgaatgcct
     9481 ctgccaagcg gtcggccagg gccttgacca tgatcgcgtt gtagtcgtcg tggtcctgtt
     9541 cgaaagctcg cgaaagggct tcggcgccta tgccggtcgt gaccgcgaag gcgccaatgt
     9601 agtcggcgat ccccgaatcg attggggcaa cgaagtccga caacgagaag tacggctgct
     9661 cgttgggctt gaccccctgc tgtcgcagca tgtggaaacg caaacgttct ttctcgcgtg
     9721 cctcgtcatg ccagacgacg atatcgtccc catcctgatt ggccggccag aagccgtaga
     9781 ccccgcgggg agtgagaagc ttctccgcga tgattcgatc gagcaactcg cgtccgtgct
     9841 gatagagctc ccgcgctgca ggtccgtagc tttcgtgctc cagtatcttc ggaaacttcc
     9901 ccctcagctc ccaggcggag aaaaagaacg tccagtcgat gtactcggcg atctcttcga
     9961 ggctcacctc gtcgatgatg cggcgccctc gaaacgcggg ggttggcaga ccctggcttg
    10021 cccagtcgat cgggacgtga ttttcccggg cgacttcaat cgagacgagc tcgcgattct
    10081 ggcggtgttc gaagagcttg cgggacttct cttgttcttc acgattcttc gcatcgaact
    10141 cagcacgacg ttcgggactc aagaggcctg agaccgcatt gacgacgcga gaagcgtcga
    10201 ggacgtgcat ggtgctcccc tcgtacgccg gagcgatttt aaccgcagtg tgagcacgac
    10261 tcgtcgttgc cccgccgatc agaagcggca atttcatccc tcgacgctgc atttcagaag
    10321 cgacgttcac catctcgtcg agcgagggcg tgatcagacc actcaatccg acgatgtcgc
    10381 aaccttccgc aatcgcggtg tcgagaatct tgtcggctgg caccatcacc ccgaggtcga
    10441 cgaccgcgta gttgttgcat ccgagaacga ccccgacgat gttcttgccg atgtcgtgca
    10501 catcgccttt gacagtggcc atcagcacct ttccctgcgc cgaaccatcc gccttctcct
    10561 cttccatgaa cggttcgagg tgggcgacgg ccttcttcat cactcgtgcg ctcttcacga
    10621 cctggggaag aaacatcttc cctgcaccga agaggtcccc aaccacggcc atcccgctca
    10681 tgaggggccc ttcgatcacg tcgagcggcc gggggtactt cagccgcgct tcttcggtgt
    10741 cttcgacgac gaactcgacg acgcctttga ccaaggcgtg cgagagacgc tcctcgactg
    10801 gtgcgtctcg ccagctgagg tcgtgaactt gtgtcttacc cttgcccttg accgtctcgg
    10861 ccagagtgac gagccgctcg gtggcatcag cgcggcggtt gaagagaacg tcctcgacgt
    10921 gctcgagaag ctctttcgga atttcctcgt atacctcgag ctgcccagcg ttcacgatgc
    10981 ccatgtccat gccggacgca atcgcatggt acaaaaacgc ggagtgcatc gcctcgcgga
    11041 tgcggtcgtt cccacggaaa gagaagctca gattgctcac accgccgctg atgagcgcgc
    11101 cggggcagcg cttcttgatc tgcttcgtcg cttcgaggaa gttgatcgcg tagtcgttgt
    11161 gctcttcgat tcctgtcgcg atcgcaaaaa tgttcggatc gaagatgatg tccgtgggcg
    11221 cgaaacccac ctcttcgacc agaatccgat acgcacggga gcaaatttcg accttccgct
    11281 cgatcgtgtc ggcctgcccc tcctcgtcga acgccatgac gacggcggcg gcaccgtacc
    11341 tccgcacctc cctcgctctc tctaggaact cgggctcacc ctctttcatg ctgatcgagt
    11401 tgacgatcga tttgccttgg acgcacttga ggccagcctg gagaacgctc cacttcgagc
    11461 tgtcgagcat gatcggcacg cgcgagatct caggctcagc accaatgaga ttcaggaacg
    11521 tggtcattgc ttgctccgag tcgagcatgc cttcgtccat gttgatatcg atgatgttgg
    11581 cgccgctgcg aacctgctga agggccaccg acaccgcctc tgggtagtcg ccttgagtga
    11641 ttaactttcg gaatctcgct gagccggtca cgttggttcg ctcaccgacc atcatgaaat
    11701 tcgtctcggg tcgaatgatg agcggctcga ggccgctgaa gcgaggatag tgcgtttcgc
    11761 gatgcggctt tggccgtgga gccacgtcgc gaacggcttc ggaaatggcg cggatgtggt
    11821 ctggagtcgt gccgcagcaa cctccgatga tgttgatcat cccgtctgcg gcaaaaccac
    11881 gaagtagccc ggccatttcc tccggcgtct ggtcgtagcc gccaaaggca ttcggaaggc
    11941 ccgcattcgg atagcaatga acgtagcaat ccgcgattcc agcgagctct ttcaagaatg
    12001 gacgcatttc ctgagcgcca agcgagcagt tgatgcccac ggccaaaggc tctgcatgtt
    12061 cgatcgagat gtaaaacgcc tcgacggtct gtccagacag agtgcgaccg ctcttgtcga
    12121 cgatggtaac cgagatgacg atcggaaggc gaacgccctt ctctccgaac acgtcgtcga
    12181 tcgcgcagag agctgccttc gcgttgagtg tgtcgaaaat tgtttcgacg aggagcagat
    12241 ccgagccgcc gtcgatgaga ccgcggacct gttcagcgta ggccttcctc gcttggtcga
    12301 aggtgagtgc gcggtagcca gggtcgttga cgtctgggct gaggctcaac gtcttgttga
    12361 gcggaccaat cgcgcccgcg acgaagcagg gcttgtcgga acggctggcg gtcgcttctt
    12421 tggcaagacg cgccgcggcg acgttgagat cgtacacgac ttcctgaagg tcgtagtccg
    12481 cttgagagaa cgcattggcg ttgaacgtgt tcgtttcgat gatgtcggcg cctgcatcga
    12541 gatacgcttc gtgaattccc cggatcacgt ctggtcgtgt gaggaccagc aggtcgttgt
    12601 tcccttgcaa gctgcttgga tggtcgcgaa atcggtcgcc ccggaagtcg gattcgccga
    12661 ggccggcgcg ctggatcatg gtgcccatcg cgccatcgaa aaatacgatg cgttgctcga
    12721 tgagctgctt cagccgttct gtcgttgcgt cgtcactcat gcgctcagat cttggtgaga
    12781 ggaactccag taatgaccca aaaagccggc ttgtgcccgt acaaccgggc ggccagcagt
    12841 caatcctgat atattcctga tagcccaaga taggatactt agactggtgc gcgctcgatg
    12901 aattccaaca gactggcttt tggcacggcc tgggttcgct tcctcctcgc ggcctgcgga
    12961 atctggctcg tccccttgat ctatccgccg atgagcgccc atcgctgggt cttcgtcgcg
    13021 tacgctggag tcgcgctcgt cgagcagttt ctcatctggc gggacatcgc gggaccttgg
    13081 cggcccgtcc ttggcggcat cctcgacatg gcgctgctga cgttcatcgt tcagcgcatc
    13141 ggttcttctc gaacggctat cgtagcgctt tacttcgtag ccggtatcgt caactcgctc
    13201 gtggtcacgc cgagactggg ggtcctgctc tcgagcatcg gtgcagcgat gtactcatcg
    13261 gtcgtcatga tggaggttcg cggcgttctt ccattcgcgc cggatggccc gctctggctg
    13321 caggcgaagc ctcccaccgc gctcgaaggg ttcatgcaag cgttcctcgt cacgatcctc
    13381 ctgatgtttg gaacggcgat cgtggcgact ctgattcaga agatcaatca acgcgaaagt
    13441 caactcctag tcgccaacga acgactcgcc gagctctcgc gtcaggatcc tctcaccaag
    13501 ctctggaatc gccgccatat tctgagccac atcgagcagg ggctggcctg gttgaatcgt
    13561 ggccggccga tggcagtcgt gatgatcgac ctcgaccagt tcaagcgggt caatgacgac
    13621 cgtggacacc tcgagggcga tcaagtcctc caagacgtgg ctgatgcgct tcggaggtcg
    13681 acgagagagg tcgacatcgc cggccgctac ggcggggacg aattcgtgat cgtgcttccg
    13741 gacacgaacc aagaagaagg tcgggttgcc ggcaaccgct tcgtcactgc gattcgagaa
    13801 gtcggcatga agttcgaccc ggcgagcccg gtgaccgcca gcgcgggcct cagcatcgcg
    13861 cgccccgggg acacggtcga ggccgtcttg aaacgcgccg atgattttgc atattcagcc
    13921 aaaggcaagg gcggcaaccg cctttgtggg tgagcgatgt ctgataaggt cttgatcgcc
    13981 ggctgcggcg acgtgggaaa cgcgcttgca aagcgcctcc tcgccgaagg gtgcgatgtt
    14041 tggggcgtgc gccggcgggt cgaagccttg gccgatggcg ttcagccgtg gcgaatcgac
    14101 ctgatgaacg accctcgctc ttttggcgcg cctcctgcgg cgttcgacta tctgttctac
    14161 acggccagcg cggaccggcg cgaggaggat gcctaccggg cgatctacgt cgacggcctg
    14221 cgcaatctct ggactgcgct tcgggatgca cgctgcaaat tgcgcagagt cctcttcact
    14281 tcgagcacgg cagtgtacgg ccagtcgaaa ggagaatggg tcgacgaaaa ctctgcgacg
    14341 gagcctcagg gatttaatgg tcgaatcctg ctcgaagcag aggccgtcgt tcgcgaaact
    14401 ccggaagagg ggatcagctt gaggctttcg gggatctacg gacccggaag aacgaggctc
    14461 gtccggaagg tctggagtgg acaagcgacc gcgaccggta gttggaccaa tcggatccac
    14521 gtcgatgact gcgccggagc gctgcaccac ctgatgcgcg tcgagaatcc ccactcgctc
    14581 tatctgggat ccgacgatcg ccccgccacg accgcagagg tcgtgacctg gctcagcaac
    14641 gagttgcgtg tccccactcc gccaaacgcg gaagccgagc aattgaacaa gcgttgtcgc
    14701 acctcgaggc tccgagagag cggttatcga ttcgcgtatc caacctaccg ggagggatat
    14761 gggccgatcg tgcgagagtt cctaagccaa actggcgtcg acggatgatc gctcaagcgg
    14821 tccggcgaat gatgtgaaaa ccaaatggcg tttcgacgac tccgctcgtg ccgcccacgt
    14881 cgagggcaaa agccgcatct tcgaaaccct tgaccatttg cccgcgaccg aacgcgccta
    14941 agtcgccacc cgccgccgcc gagccacaat cggagtgctc acgcgcaagc gcggcaaaat
    15001 cggcgccgac tcgaagctgt tgctcgatgt ctgcaatctg agattgggcg ttttccttgc
    15061 tgcgtgtagc cgaggagcgc tgagagccgg cgtacatgag taggatgtgt gaggcgttga
    15121 tctgatctgc catggtcgtc tcccttcgag gcggagaccc tagcagaaat cgggatttcg
    15181 caaggtgact aaatcggtcg gagcttgctc acggaaagcg atctggcagc ggcacgatcg
    15241 accttccccg ttcgattcag cggaagagcg ttcagcagcg cgattcgcct gggtcgcttg
    15301 tgtgtcgcaa gctcgttcgt cattcgatcg cgcaacgcgt ctccgctgta ctccgctccc
    15361 tcgatcacga tagcagcaca tacttcctct ccccattcct cgtgtggctg tgagaagacg
    15421 caagccatcg cgatgccagg aacggtctct agccaggctt cgacctccgc aggagcgacg
    15481 ttctctccgc ccgtcacgat gaggtcatcg attcgcccca gaacgagaag ttgaccatca
    15541 gggaggaacg aaccgaaatc tccggtgcgc agccatccgt ctcgagtcca tggctcggcg
    15601 cttcggtcct ctccgaggta gccgtccatg agaacgtccc cgcggacttg aatctcgccg
    15661 gactcgatcc gcacgtcgac tccaggaact gggcgaccgg agcctggttg cccggtttgc
    15721 atgggggtct gggtcgagat ttgagaacag gcttcggtgc aaccgtaggt cgccaaggtc
    15781 gggacctttc gctccgtcgc gagctcacga agcgcatcgg gaaacggggc gccgccgacc
    15841 aagacggcgc gcagcccggt gcctggcttc cactcgggtt gcgcgagctg cagcagtcgc
    15901 cggagcatcg tcggtaccac gctcagcaac gtgacgtcct gggtctccat gatctgcgcc
    15961 acttctcgcg gatcgaaagc cgtgggccca aggaccacgc agcgcctggc gatcaaggaa
    16021 cgcgtcacga tcgaaagacc acctacgtgt gcgagtggca tagtcagcag ccaacgatcg
    16081 tcgggccgcc aatcgaggtt ttcggcgtgg gccgatgcgg acgcaatgaa cgcccgacgc
    16141 gacaaacgcg cgcctcgcgg ccggcctttg gatcccgatg tgtacacaat cgccaaggtc
    16201 gagtccgaag cgatcgattc ggagcgctgc ggagccacgg tttggagatc cctcggaagc
    16261 ggctcggcga tcacatgcac gggctcagcc tcctcgagga cgagggactg ttcgcgactg
    16321 ctgaggcgcg gatgcaggag cacggccgga catccgagct cgaaaagcgc atagagcgag
    16381 acgatcgagt cgaggtcgac cctgggggtg agcgcaacgc gatcccccgg cgccacgcct
    16441 ctcgcaatca gtgccgagag agcggcgcac accctatcgg ctagatcgcc gtacgaccaa
    16501 atcgtcccat tcacgacgag gcagtcgcga tgcggcgcct cgttcgcggc ggcgagcaag
    16561 ctcagctcat ccatcggcga gctcttcaaa ttggaggccc aaacccggct catcatgtgg
    16621 cacgatctcg cggccttcga tggcggcgat tcgatgcggt ggccaaagcg caagcgcggg
    16681 gtgcgcgcca aggcccgcgg caagctctgt ttgaagcgcc agcgcgagct ccgcggtgac
    16741 agctctcccg atcggcccgt cgaagctgtg agacaccagg tatggcacag cgtgacaggc
    16801 tgcatgctcg gcggccgcaa acgcggcgcg aagcccaccg atcaccatcg gtttgatgac
    16861 gacagcgcgg atctttccgg tatcgaggag gcgctcggtc aagtccggat cacgcaaggt
    16921 ctcgtccagc gcgaacggca gcggaaggct caaagccgcg atccagtcgc cgggtgtcac
    16981 cggctcttcg attagctcca gcccgaggga ctcgagtacc gcgctgtgct tgcgaagcgc
    17041 gctgagcgac acccgctggt tgccatcgag ccggagagca acgctcggat gcgtctctcg
    17101 aatcgttcga agcgctctga gctccgtctc gagatccggg ccgatcttga gcttcaggtg
    17161 ggtggcaccg tctgcgatca gcgcgtccgc tcgcgccggc cagctcccgg gatctggatc
    17221 catgaccaag tcggcgattg gaatgcgctg cctttccgca tcgccggcta gaacccgatg
    17281 caatggttct ccgcgggttt ggccgagcca gtctaagagc gccgtttcga atgccaatct
    17341 cgacgcaggg tgcgccattg gatgcgcggc gaacgtctcg gtcaggagct cgagcgggcc
    17401 cgcgagtggg ttgacccgaa tcggtccctc ggcgaggcgc tgaagctcgc tcgccacctc
    17461 atcgatggaa tccagcgaat atccgggcaa tggggaggcc tcgccctgtc cgctcgtacc
    17521 gtcgctccgc atgagtcgaa gcaccgcgaa ggttcgctcc tcgagcccgg ttgccgcgcc
    17581 gaccagcgat tgcgaaagct tcccttcgag aacgtcgatc tgggcgtgga gctcggtcat
    17641 agcgcgattc cgatcgcgaa cagaacgccg aagatcgaga gcagcttcgc ggttccggac
    17701 agggtccggt tgagggccac gccccgatcc gcgacgaccg aatgatacaa gcgggcggcg
    17761 attggaatcg tgagcagtgg aagcaagacc catgcgctgg tccagccaag tccgaggaga
    17821 accatgggcg tggcgtacga cagcaagagt aagaagccgt actccaggac acctcggctc
    17881 cgcccgaagc gaaccacgag ggtgcccttc cctgcgcggg catccccttc gaagtcgcgc
    17941 acgttgttca cgacgagaat cgccgtcgcc aaggatccga ttggcagcga tgcaagccag
    18001 gccacctctg gaacgaacga ggcttgcacg aaggcggtgc ccgcaacagc gacgaatccg
    18061 aagaatgcca tcacgaagac atcgccgaga ccgttgtaag cgagcgggaa agggccgccg
    18121 gtgtaggcca gacctgcggc catcgacgcc agaccaatca ggatcacgac cggaccggcg
    18181 agccaagtca gataaatgcc ggttccgagc gccaaggcaa aggccaagat gatgccctgc
    18241 ttcagctgcc ccgtggtcag caagccggcc tgcgcggtac gtgttggtcc gaggcgttcc
    18301 tcgtcgtcgg ctcccttttg gaagtcgaac atgtcgtttg cgaagtttgt cgcgatctgg
    18361 attaagaatg cgccgagaag ggcggcaagg gccgcgtccc agcggaagcg gttcacgctc
    18421 catgcgcaag cggtgcctac ggcgaccgga gcgaacgccg cggtgagtgt cgcgggacgc
    18481 gccgcaagaa accatgcgcc aaccgagccc ggtgcgggat tcgccatcag gcaacctgcc
    18541 tctcgacgcg gttcagcaat tcggcgagag cgtctggcgc ctcgaggacg acgttgtggc
    18601 cgacgcccgg gatgcgctcc acgccgatgg tcgggttcgc tgcccgaagt gtctctgcga
    18661 tcgcggagaa tttcgaatcg agcgcgccgg tcaccagtgt gattggcgtc tcataggagc
    18721 gcatcaaggc tcgatagtcg ggcatttcgg ccaagcccag aagctccagc gcgagagcca
    18781 gcccctctgc ctcgtggtcg aggcgaatgg cgcgttgctc cgccaaggtc tctgcggaaa
    18841 gcgtgcgctg gctcgagaac agcggcacgt cttcccaagc gtcgacgaag gcggcgagac
    18901 cctggtctcg cagcaagcgc gagtaagccg aatcggcctc tcgccgcgct ccgcggtcac
    18961 tttggctatc gaggcctgga tggacgccga ccagtacggc tgcatcgaag aggcgcggcc
    19021 gacgggcgag aaggcccaac gcgatccgtg cacccatgga atagccgcaa accaatcgcg
    19081 gctgctccgt gctttcaatt tcggaggcca ggcgctcgag ctcgcgctcg aacgaatcga
    19141 ccttcctatg tcgccagttt ggaccatgac ccgcgagggt tgccagcagc gggggtctct
    19201 cgaaccgcgc tagctcgacg agatgggtcc agctccgtgg agagcctgcg aagccatgga
    19261 tcagcgtcca catcaagagc tctcggaacg aagcgctcgc tcgactcgac ggatgagctc
    19321 acggctctgt tctcgcgcgc tctgagtagg caccagcgct tcgacgacag tgcacccggc
    19381 gcggccgtac gcgctgtcga gcgcgcgacg gaagccgctc acggtgttcg cgctctcgaa
    19441 ttcgcagccg tagacccgcg cggcgctctc gaggtctgcg ccatgcggcg tggtgaagta
    19501 cgaaagccag ctctctttgc cctgctgcgc gatgggcagc tgctggaaga tctgtccccc
    19561 agcgttgttg accaccacga tgaccagagg tccatccagg cgcgatgcca agtcgagccc
    19621 attgatgtcg tgaagaaagc tgacgtcacc gatcagcagc gtggtcggtc cctcgacgac
    19681 cgaagccacg ccggccgcgc ccgacacgac gccgtcgatc ccgctgacac cacgctggga
    19741 atggacgagg agcagcttgt cgctcggcgg cgcccagatg tcggcgtcac gaatcgccag
    19801 actattgccc aagaccaggg cgctcggact gggcagcgca tctagcagcg cccgcgccgc
    19861 gccggcctga ctgagtgcat ctcccgactg ctcgaggatc gcagagacct cgtcccagac
    19921 cgcggactct gcccattcga aacccggtag gaactccggg tggcgacggt tcaggtggta
    19981 ctccgcttcg cagagtccca cgaggaacga gccaatatcc gcctggacga cggcttcggc
    20041 gtccgagctc gggtcggccc atttccacgg atggacgacg atccgacgaa tccgctcctc
    20101 ggcacaaatc cgttcccagc ctacggagac gggcgtcgct ccgagctgaa gcacgaaatc
    20161 cggtcggcaa agcgacctcc cgttggcagt ctgccaaatc gtatcgaacc ctccgagcgc
    20221 tcgcgacttc gcccagcatc ggaactgact cgaggtttcg gcgagcacga ccgcaccgga
    20281 acgttcagcg agctcatcga gcgcatcgac gaacatcgca ctccgcagat ccgccggacc
    20341 gcagagaatg accgggcgtg ccgctgaatc gagcgcctcg gcgaccgagt cggtcaccaa
    20401 gctgggaacc agctcgacga gcttgggatc gagcggacgt gctcgatgga gctgggtgag
    20461 cggctccgcg agcactgcgt ctacccgggc atgaagctca ctcgctaccg ggaccggctc
    20521 gagaggctta cgcgcttgcg cgttgatgtg gaccggtccc ggcttcgcgc cgagggcctc
    20581 agaaaccgct tgagcaatga tccttcgcgc cgcccgaagg cccagcgcat catcccgcgg
    20641 ctctcccaga ttggcgaaga agcggacctt ggttccgaag agacccactt gatccgtggt
    20701 ttggttcgcc ccacatccgt ggagctctgg cggccggtca gcagaaagca cgatcaaggg
    20761 aacgccggct tcactcgcct cgacgaccgc agggaagtag ttggcgggtg cgcttccgga
    20821 agtacagacg aggagagagg ggcgaagcgc gccgcgcgcc tgaccgagcg catagtgcgc
    20881 agcggaacgc tcgtcgatcg cgctcgtaca ctcgagccct tcgtgctcga gggcggcgat
    20941 cacgaacggc gtggaacgcg accctggact gatgatcgcg tcaccgacgc cggcttggac
    21001 gaagctgtcg atgacgagcc tcgcccactc cgtctgtaac tcagcgactg tcgccatgga
    21061 gcgctccaag aagcgcctgc atcttgagct cggtctcggt gtattcggcc tcgggatcgg
    21121 agtcggccat gattccggcg ccagcgtaca cccaggcctt cccgtcgtga agcaatccag
    21181 agcgaagggc taccacgaac tcgccgtccc c
//
LOCUS       DASRZF010000161        16577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3892825, whole genome shotgun sequence.
ACCESSION   DASRZF010000161 DASRZF010000000
VERSION     DASRZF010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 16577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16577
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3892825"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..1196)
                     /locus_tag="VFG22_07290"
     CDS             complement(177..1196)
                     /locus_tag="VFG22_07290"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET6416084.1"
                     /translation="MTVSLVTGGCGFVGAAIARALQARGDTVIIVDVAPRCPVEGADY
                     RRIDITDKAAVTEACRGVDAIIHNASIVHTKQNKHDVIWGVNLGGTENMLEAARQHGV
                     PRFIYISSGSVVYEGKDIENGDESLPYSSVSQAPYADSKIAAEKLVLAENGRGGMATC
                     ALRPHVIFGPGDNRFMPTLLEKGRNGQLRVQIGRGIWLSDYTYVSNLTDAVLLADEAL
                     AKDGLESIAAGQAYFITNGEPMPFWDFIRKVAARLGFPPVKYRAPKSLIYAIAAVKEG
                     IDTLKGGTLNAEDGLTRFAIRYICTHHYFSIEKARRELGYDPSVSVDEGIELTCQHLE
                     ATGAV"
     gene            complement(1200..1874)
                     /locus_tag="VFG22_07295"
     CDS             complement(1200..1874)
                     /locus_tag="VFG22_07295"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HET6416085.1"
                     /translation="MKVLYVILVSYASAGLAQAALFYAYRSKNAQQYLISDDPHRSKS
                     DRELHWRVALNISVSITLIFGVMFGLGEYLYYDHAVPAWRYVLEAVTVVLIYDFAYYF
                     MHRYPFHEWKLLRGVHSVHHAARNPRFIDSLLLHPVETVLGLGLFFGSVALVGGVHLY
                     TLGALFTTYTALNILNHAGLDVPHFPFKTLGVLAAKHDRHHHSMLSGNYASITPLPDI
                     IFGTVE"
     gene            complement(1903..3066)
                     /locus_tag="VFG22_07300"
     CDS             complement(1903..3066)
                     /locus_tag="VFG22_07300"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HET6416086.1"
                     /translation="MSVAFHELKVSAVIRETSECSSFELLVPAELEDAYRWKPGQHIT
                     IGIPWADFEVARCYSISSCPELGETLKFAVKRVEGGRVSNWLNDNLKPGDAIKVSVPE
                     GRFVLDPDTRSGAPLFLFAGGSGITPVIALLKSALFGTRRAVILLYANQDQESVIYRA
                     EIDRLAVENPDRLTVRHHFDSENGYLSPQDIRSLLHHTDIAEYYVCGPEPFMELIETT
                     LHSAGVHEELVYFERFVSPADPDRVETDLVEIESLEAPETFSLWLGGRSRTVPYVRGK
                     TLLECAQAAGLQPSHSCESGYCGSCMAHVNTGKVHMRTHEALSDRDIARGVALLCQSI
                     PAGREPLELDSDSTSFRAASAVKSSYSKRASQFAAVAVFAFMVAGTLILRFVH"
     gene            complement(3063..3671)
                     /locus_tag="VFG22_07305"
     CDS             complement(3063..3671)
                     /locus_tag="VFG22_07305"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6416087.1"
                     /translation="MLDAAERVFGEEGYEATKVQRIASEAGVSLTTLYSVVESKWEIY
                     RAVHRRRLNEVMRLTEAVLRRELSSLETLIAGTRMQLGFFMTRRDFTKMQLKEVTAWS
                     TIELLRSPEQVEALGAGLKLFGELLRQCIEDGFLVDDDPELMARMIIASQQVRLAMWM
                     DRECKEDPERVSDAALRHLLRSYCKAEKLAAALHAAGLEDAP"
     gene            3780..5372
                     /locus_tag="VFG22_07310"
     CDS             3780..5372
                     /locus_tag="VFG22_07310"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET6416088.1"
                     /translation="MSIPISAGSRSSISNESNEIACLDPATRQRLGTVRIDSPEDVDA
                     AVARAKVAQRAWRKTSFEERRELLRELLAYTVEQKDEICIAVQRDSGKTRENALMGEI
                     WPTCEKFRWMIKNGEKYLRPEPVSSGLLVHKKARLEYHPLGVVAAIIPWNYPFQNIVN
                     PLIPALMAGNAIVLKPSEWVAWSSARFIDELRTVIETAGHSPDLVQAVQGYGPTGAAL
                     ARSAVDTILFIGSVQNGRRVIEASAERVIPVVMELGGKDAFIVCEDADLEQAVHAALA
                     GCYINCGQNCVASERILVYDSIYEAFEKRAADLVDGFRQGSSRESLVDIGAIVTPLQL
                     EVIERAVDGAVAQGARIVRGGKRTLNEKGDYFEPTILADVTADMDIAQDEVFGPVMLL
                     MRVHDDAEAVKVANGVSYGLSSSVFSKDRARARRIASELEAGMSAINEFAGITYMAQG
                     LTFGGVKASGFGRMNGREGLRSMCNIKSVVEDRLPIHQANRIFPIAERDYGLFSGVIE
                     LIYGRGFGQRWRGFLKLFGRKS"
     gene            5372..6967
                     /locus_tag="VFG22_07315"
     CDS             5372..6967
                     /locus_tag="VFG22_07315"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HET6416089.1"
                     /translation="MEEFDYIVVGGGSAGCIVASRLADDARLKILLLESGPSAEEHPE
                     TLVAAGYKEAFINDAVMGERFSVPQVHSARQRVFAGTGTVLGGSGSVNGMVYTRGARE
                     DYAEWPKGWQWDEIQDEFQAIEKTLRPHQRPPTNWTEACIASAEQNGFTRKRDLNDGD
                     MHNAIGYEWMSYEGEERRSSYVAFMKDVGPRPNLTVITGAHVHRIVFNAAKRARAVEF
                     EHEGALKRASAVTEIILSAGALETPKILMLSGVGPSEHLRAFGIPVVADRPSVGEGLH
                     DHPNVPVFFKTKAEIDCFYPQVYSFFRTNEDSDLPKSQSDTCYVYWPAPSAMRQMMQR
                     MLPPMVVPNSLYGPRSRQIVRSLVGTAFKIGAIQRFTDQMFGVILILGKPKSRGTLRL
                     ESADARAQASIDPAYYSHPEDLHTMVKGVRIARAIGSSNGLAAWNVKELMPGKRVNSD
                     ESIANYIRRNTITTYHFAGTCGMGTLERDAVDPELRLRGVSGLRIADASAIPWTPVSA
                     LNAPSMLIGYRASELILNGATLS"
     gene            7039..8052
                     /gene="dctP"
                     /locus_tag="VFG22_07320"
     CDS             7039..8052
                     /gene="dctP"
                     /locus_tag="VFG22_07320"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HET6416090.1"
                     /translation="MNKAKHAFIALLAASAFFLPGAVSVPAANAASPIIIRFASLAPP
                     GSAFMKLMQAWNRSLKKETENRVQLRFYSGGSQGDERDFIRKVRAGQMDAAGVTTTGL
                     GMVARPVLVLTAPGMITEYDQLERARAKLSDRFAKLFEEAGFVMLAWGEAGKNRIFSM
                     EEFAKPEDLKKLRPWAWKDDPVFAGYISTIGANAVRVGVPEVYGGLQTRMLDTVPASA
                     LAAVALQWYTRLNYMAKTNFGIIIGGSLIKQEKFDELTEHDQKVLLDTSERAARAGDV
                     MARRDDDRAYASLLKRGMIEVDTNEYKAEWDAVAKTTRENLTGRVYSKSLLEAVEAAI
                     APN"
     gene            complement(8078..8914)
                     /locus_tag="VFG22_07325"
     CDS             complement(8078..8914)
                     /locus_tag="VFG22_07325"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6416091.1"
                     /translation="MVVEAVPEGKYVDVGSGITLHYHQAGTGDQGTVLFIHGSGPGAS
                     GWSNFKGNYPILAEHGYRAIVPDTMGYGYSSKPEEGTFSLDDVAAQYKALLDALDVAR
                     VTVVGNSQGGAIAISMALNYPQLVQKLVLMAPGGLETRETYMAMEGIKAMIRVLYKEG
                     VSKETMRRVFALQLHDETKITDEVIEERYQVAMTQHKDNIARIHVTNQEDRLSEIHCP
                     VLCFWGANDKFCPSSGAAKIASRCSSSRTMLISSCGHWVMVEYPKLFNELTLKFLADD
                     LG"
     gene            complement(8976..9899)
                     /locus_tag="VFG22_07330"
     CDS             complement(8976..9899)
                     /locus_tag="VFG22_07330"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6416092.1"
                     /translation="MSAVKQLGYLVFEVSDLAAWERFGTQILGLQLVDERPDAGFGLR
                     MDGYRQRFFVERGPADDLVAIGWEVSGPEELGRMIDRLRSAGHEVTECTDEEAAKRNV
                     EALIRFTDPAGNPVEVFHSPQMAAEPFVSDIVRSGFVADEQGFGHVVVVSKDMDEHEA
                     FYATMFGLKLSDYIRCEYFGHKIDVTFMHANARHHSLAFGGAQLKRIHHFMVQVKSID
                     EVGMCYDRFLFSGGMVHQTLGRHPNDKMFSFYGYTPSGFHFEFGAGAIEIDEETWETK
                     THGKVSEWGHHPPQLLTKAYRRAKEAGEKKH"
     gene            9961..10662
                     /locus_tag="VFG22_07335"
     CDS             9961..10662
                     /locus_tag="VFG22_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416093.1"
                     /translation="MESSTKLRGPTLPTAAVIGIAVVLGTSFHVWLHHGVHGVYNPRQ
                     IGLAFFLVINVLVAWWEIALFVCQDQIRTEYDATQEPYRGREMTRIAEVFARPIPIFR
                     VFCFCEWSNIWSSYSLFDPGYSDRRSFGYNIDVGNGFTTLIPALLFAFGMTFELMPAR
                     VLGIMGVIVFWQMFYGTAVYFFQFFNNRRHRGHSLRDLLLFVGLTNLMWFVFPLWGLS
                     TSIQLIMDGSYALFV"
     gene            complement(10659..12038)
                     /locus_tag="VFG22_07340"
     CDS             complement(10659..12038)
                     /locus_tag="VFG22_07340"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6416094.1"
                     /translation="MSEKIGIVGGGIAGVAAAWSLHRAGFEVELFEKGPALGGNAKTF
                     RWPAREGHAESPLLVVAWPQMYYHNYELLLDELGVGIETLPISYFVQTPDGYFCQDGA
                     TELHRRFEDDFRRWRKLVAFVSRVNSFFLPKERHDSLYHFSYFNPLNLIPLYWLARLF
                     GVSSDFWVTIFVPIHCASFITTSMRGVPAVILPLLESIVPLEEPTQMGTWRGAPRQVF
                     ERMTAPFSDKVHTDHEITSVKRRNGGFSIGDLQGRTYQVDKLVFACDASSVLDALESP
                     TWLQKALFSNAQYVDDVDPTFSRFIVHSDTSIFPEKHRARILSDYNTYSEIDDAGELE
                     CTFVISAQNPSTKDAGVPMLVTFNSKKAIGEVHKRIELPHPTHTVSLRNLIIMSLMKF
                     LQGKGDIHYCGTFTTPEGGHDLSFMSGLVAASAIGAPYPFAGKSPNAVADFNQMQRMM
                     LRARARRLV"
     gene            complement(12063..13307)
                     /locus_tag="VFG22_07345"
     CDS             complement(12063..13307)
                     /locus_tag="VFG22_07345"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET6416095.1"
                     /translation="MAELIEIRGRYEPRFEPVAKLMRKQISQYGGGAAAAVFLDGKPV
                     VDIWAGPARHDGTPWKQDTMTICYSGTKGIVSTAIHMLATDGLIDYEAPVAEYWPEFG
                     CKGKERITVRQLLSHQAGLHKIVHLVEKLTDILDWDLIVSRLEKTEPDFAPGTANAYQ
                     AMTFGWLVGELIRRISGLTVPEFLAQRIVEPLGLDGLFIGNADTEMDRIPDFVGLPQL
                     QRSGAKRLSIDYSIPFWVRSKRMRDLCRRGLTPRNAKQLFSHPAFWKASLPAMNGVAT
                     ARSLAKMYAVLSMGGELDGVRLVSPEIIAKATEVQTKRADKVTIYPLHWKLGYHRADA
                     FLLDVPESFGHFGLGGAGGWANPEHRLAFAFLHSSFPVSIIGQTRTVMMTAAVYESLG
                     IYKGLCHTLRHGPIVDLVPKNH"
     gene            complement(13339..14151)
                     /locus_tag="VFG22_07350"
     CDS             complement(13339..14151)
                     /locus_tag="VFG22_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416096.1"
                     /translation="MTTAHSPRWFSVNPSKAWGEKFFLVYSPIWMTFMGVVMASGTTK
                     RIGELGFLAIGIAVALPLLVVPALIRDERPIGRRWYQTYWFKANLYVAIFNFAANYFG
                     SEYFFDVLGMVYDYPMIEINLDAALVGSGVQRVPIIMYLLTQAYFLTYHTTAVIVLRR
                     IRTSGLPIGPLLWPILLLSVAYFWAWMETKAMANPWIEAQFYYRDMDRMLRYGSLFYS
                     LYFIASFPIFYQLDEDRDRNWSLQITCAAALSASMIMMFLLDFAASVFGPLT"
     gene            complement(14148..15527)
                     /locus_tag="VFG22_07355"
     CDS             complement(14148..15527)
                     /locus_tag="VFG22_07355"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET6416097.1"
                     /translation="MTARAQATSAPLGGGMLKQAPKLKGGLPLIGHTMDFVKDTIGLL
                     QRTYEECGKVGRFKLFGKDMILFTGPEAHEALFRQPDEVLSPNAAYKMMVPVFGEGVA
                     YGAEPAKMAEQMHMLYPALRNNRMRTYTEIVARETLDVLADWGDSGEIEASAFFGMLT
                     NFTSSSCLLGKEFRNEMSEEFSKVYYDLERGIVAWGYIHPYLPIPAFRRRDRARARLG
                     HMVSEIVEKRRQSGYRGEDFLQTLMDAEYSDGSKLSDHEITGMLVAAMFAGHHTSSVT
                     TAWTLIELLRHPRFLEEVRGEIRRSYSANEDYTFQSLRDLEKTEAAVKEALRIHPPLF
                     ILLRAALEDCEILGYRVKKGNWVAVSPMVAHRDEDVFKNALEYDPDRYGPGREEDKRP
                     FAYISFGGGRHKCMGNAFAILQIKTIIAVLLNRFDFELVGDPIETDFQGLVLGPKPPI
                     RIRYRRRAA"
     gene            complement(15524..16147)
                     /locus_tag="VFG22_07360"
     CDS             complement(15524..16147)
                     /locus_tag="VFG22_07360"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6416098.1"
                     /translation="MSEAIARARSAKASMYRSLVIDSAEHLFATQGYEKTKIQDIAAA
                     SGLSLGTLYSVFAGKADIHEAVHDERLAELFRLTGHAMKSEASAAERLLAGNRVFVRW
                     LTEHPDYLRIHLDAGGAWSSDPQQVGEGLVAAWHRGIDLMARVLEEAMRDGDVYQADP
                     VITARLMAAIQQVFMSSWFDTGMEDSATSLVQQIERQLRRSLFRSGS"
     gene            16234..16425
                     /locus_tag="VFG22_07365"
     CDS             16234..16425
                     /locus_tag="VFG22_07365"
                     /inference="COORDINATES: protein motif:HMM:NF018595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HET6416099.1"
                     /translation="MRIKVDFDLCQGHANCMGEAPEVFQVDEQGFLNILQEEPPEELR
                     EKVELAVKYCPTGAIGMED"
BASE COUNT         3418 a         4877 c         4972 g         3310 t
ORIGIN      
        1 tcactgatac tggacaccta cacctccaaa ccggcatatc gcttggtagg gcaactggtt
       61 cattcctgcg cgcgttcaaa ggggcgactg caggggccgg gggcgggccg cgttcggggg
      121 gactgcggcc cgtccgccaa atccggctgc agcaggattg gggcgagtcg attcgtctag
      181 acggcgcctg tggcttcgag atgctgacag gtgagctcta tgccctcgtc cacactgacc
      241 gaaggatcat agccgagctc ccgtcgtgcc ttctcgatgc tgaaataatg atgggtgcaa
      301 atgtagcgaa tggcaaaacg cgtcagaccg tcctcggcgt tgagtgtccc accctttagc
      361 gtatcgatcc cttctttgac cgccgcgatc gcatagatga gtgacttcgg cgctcggtac
      421 ttcaccgggg ggaagccgag acgggccgcg actttgcgaa tgaagtccca gaacggcatc
      481 ggttcaccgt tcgtgatgaa gtaagcttgg cccgcggcga tgctctcgag tccatctttg
      541 gcgagggctt cgtcggcgag aaggacggcg tccgtcaagt tgctcacgta ggtgtaatcg
      601 gaaagccaaa tgccccgtcc gatctgcacg cgcagctggc cgttacgccc cttctcgagc
      661 agcgtcggca tgaaccggtt gtcgcccggg ccgaagatga cgtggggccg gagcgcgcag
      721 gttgccatac ctccccgccc gttctccgcc agaaccagct tctctgcagc gatcttgctg
      781 tcggcgtagg gcgcctggga gaccgaggaa tacggcagac tctcgtctcc gttctcgatg
      841 tcctttccct cgtacacgac gctgccggag ctgatgtaaa tgaaccgcgg tacgccgtgc
      901 tgacgggcgg cttcgagcat gttctccgta ccgccgaggt tcactcccca gatcacgtcg
      961 tgcttgttct gcttcgtgtg aacgatcgaa gcgttgtgga tgatcgcatc gaccccgcgg
     1021 cacgcttcgg tgaccgctgc tttgtcggtg atgtcgattc gtcgatagtc ggccccttcg
     1081 acggggcacc tgggcgcgac gtcgacgatg atgaccgtat caccccgagc ctgaagtgcg
     1141 cgagctattg ccgcgccaac gaatccgcag ccccccgtga ccaaactgac agtcatgaat
     1201 tactcgaccg tcccgaagat gatgtcgggc aacggcgtga tggatgcgta gttgccggaa
     1261 agcatgctgt ggtggtgccg gtcgtgcttt gccgcgagaa ctcccaaagt cttgaatggg
     1321 aaatgcggga cgtcgagccc agcatgattg aggatgttga gcgcagtgta agtcgtgaag
     1381 agcgcgccca aggtgtagag gtgcacgccg ccgacgaggg ccaccgaccc gaagaacaag
     1441 ccgagcccga gaactgtttc gactgggtgc aacaagaggc tgtcgatgaa tctggggttc
     1501 ctggctgcgt ggtgaacgga gtgtaccccg cggaggagct tccattcgtg gaatggatag
     1561 cgatgcatga agtagtacgc gaagtcgtag atcaaaacta cggtcactgc ttctaggacg
     1621 taccgccaag cggggaccgc gtgatcgtag tagaggtact ctcccagccc gaacatcacc
     1681 ccgaagatca gagtgatcga aacggagatg ttcagagcga ctcgccagtg gagctcgcgg
     1741 tcgctcttgg agcgatgagg atcatcgctg atcaggtact gctgcgcgtt cttcgagcga
     1801 taggcataga acaatgccgc ctgtgcaagg cccgcggacg catagctgac caggatgacg
     1861 tacaagacct tcatgtcgat ttccaaagag acattctacc acctagtgta cgaacctcag
     1921 aatgagcgtc ccagcgacca tgaacgcaaa cacggctacg gctgcgaact gggacgcgcg
     1981 cttgctgtaa gacgacttga ccgcggacgc cgcgcgaaaa gaggtcgagt cgctgtcgag
     2041 ctcgagcggt tcgcgaccgg ctgggatgct ctggcaaaga agagccacac cccgagcgat
     2101 gtcgcggtcg gaaagcgctt cgtgcgtcct catgtgaacc ttgccggtgt ttacatgggc
     2161 catgcaggag ccacagtatc ccgactcgca ggagtgcgaa ggttgcaggc cagcggcctg
     2221 ggcgcactcg agaagagtct tgcccctgac gtatggcacc gtgcgcgacc ggccgccgag
     2281 ccaaaggctg aatgtttcgg gagcttcgag cgactctatt tcgaccaggt cggtttcgac
     2341 tcgatcggga tcagccggcg agacgaagcg ctcgaagtag acgagctctt cgtgcacgcc
     2401 tgcggagtgc agcgtcgttt cgatcagctc catgaagggt tccggtccac acacgtagta
     2461 ctcggcgatg tccgtatggt ggaggaggga ccgaatatcc tggggcgaca aatagccgtt
     2521 ctctgagtcg aagtgatgac gcactgtcag ccggtccgga ttctcgacgg caagtcggtc
     2581 gatctccgct ctgtaaatga cggattcctg atcttggttg gcatagagca gaatcaccgc
     2641 gcgcctcgtg ccgaagagcg cgctcttcag cagggcaatc actggcgtga tcccgctccc
     2701 gcccgcaaac aagaaaagcg gcgcgcccga acgggtgtcg gggtctagaa cgaagcgacc
     2761 ctcgggtacg ctgaccttga tcgcatcacc tggcttcaga ttgtcgttga gccagttcga
     2821 gactcgccct ccttcgacgc gttttactgc aaatttcaag gtttcaccga gctccgggca
     2881 gctcgaaatc gagtaacacc gagccacctc gaagtccgcc cagggaatcc cgatggtgat
     2941 gtgctggccg ggcttccagc ggtatgcatc ctcgagctcc gctggcacga gaagctcgaa
     3001 agagctgcac tctgacgtct cgcggatcac cgcagagacc ttcagctcgt gaaatgcgac
     3061 gctcacggtg catcctccag gcccgctgcg tgcagtgctg cggcgagctt ctccgctttg
     3121 caataactac gaagcagatg cctaagcgct gcatcggata cgcgctcggg gtcttccttg
     3181 cactcgcgat ccatccacat cgccaaccga acttgctgcg aggcgatgat catccgcgcc
     3241 atgagctccg ggtcgtcgtc gacgaggaaa ccgtcctcga tacactgccg aagcagctcc
     3301 ccgaagagct tcagtcctgc accgagcgcc tcgacttgct cggggctccg caagagctcg
     3361 atcgtcgacc aggcagtgac ttctttgagc tgcatcttgg tgaagtctcg acgtgtcatg
     3421 aagaacccga gttgcatgcg agtacccgcg atcagggttt cgagcgaaga cagctctcgt
     3481 cgaagaaccg cctcggtaag ccgcatgacc tcgttcaaac gccgccgatg caccgcacgg
     3541 tagatctccc acttggactc gaccacgctg taaagcgtgg tgagcgagac gccggcctcg
     3601 gacgcaattc gctggacctt ggtcgcctcg tacccctctt caccgaacac gcgctcggcg
     3661 gcgtcgagca cggcatctcg gtaaagctct cgcttcctat ctctcaggga ttcctgggct
     3721 tttggaactg ctgtgctcgt tgacacggta gggtcggaat gataaaggcg gaaatcagaa
     3781 tgtcaattcc gatttctgct gggtctcgct catcgatctc gaatgagtcg aacgagatcg
     3841 catgcttgga ccctgcgaca cggcaacggc tcgggaccgt tcgcatcgac tccccggaag
     3901 acgtggacgc cgcggtggcg cgggcgaaag tcgctcagag agcctggcgg aaaaccagct
     3961 tcgaagagcg ccgtgagctg ctccgcgagc tgctggcata cacggtcgaa cagaaagacg
     4021 agatttgtat cgccgtgcaa cgtgactccg gcaagacgcg cgagaacgcg ctgatggggg
     4081 agatctggcc aacctgcgag aagttccgct ggatgatcaa gaacggcgag aaatacctgc
     4141 gccccgagcc ggtgtcttcg ggcttgttgg tgcacaagaa ggcgcggctc gagtaccacc
     4201 cgcttggggt cgtagcggcg atcattcctt ggaactaccc gttccagaac atcgtcaatc
     4261 ctctcatccc ggcgctgatg gcggggaacg ccatcgtgct caagccgtcc gagtgggttg
     4321 cctggtcgag cgcccggttc atcgacgagt tgcgcacggt catcgagacc gccggtcaca
     4381 gtccggatct cgttcaggcc gtccaaggat acggtccgac cggcgcggca ttggcacgct
     4441 ccgccgtgga tacgattctc ttcatcggct ccgttcagaa cgggcgccgc gtcatcgagg
     4501 caagcgccga gcgcgtgatt cccgtggtca tggagctcgg cggcaaagac gcgttcatcg
     4561 tctgcgagga tgcggacctc gaacaggcag tccacgctgc tttggccggc tgctacatca
     4621 attgtggtca gaactgcgtc gcctcggagc ggattctcgt ttacgactcg atctacgagg
     4681 ccttcgagaa acgtgcagcg gacctcgtcg acggattcag gcagggcagc agtcgcgaat
     4741 ccctcgtcga tatcggggcc atcgtcacgc ccctgcagct ggaggtgatc gaaagagccg
     4801 tagacggggc ggtcgcccaa ggcgcacgca tcgtgagggg cggtaagagg acgctgaacg
     4861 agaagggcga ttatttcgag cccacgattt tggctgacgt gaccgcggac atggacatcg
     4921 cccaggacga ggtattcggg cccgtgatgc tcctgatgcg agtgcacgac gacgctgaag
     4981 cggtgaaggt cgcgaacggc gtgtcctacg gtctttcatc gtcggtgttc tcgaaggatc
     5041 gtgcgcgcgc gcgccgcatc gcctctgagc tcgaggctgg catgagcgca atcaacgagt
     5101 tcgcaggcat cacgtacatg gcgcaaggct tgaccttcgg aggcgtgaaa gcatctggtt
     5161 tcggacgcat gaatgggcgc gaagggctgc gctcgatgtg caatatcaag tccgtcgtcg
     5221 aggatcgtct tccgatccac caagccaacc gcatatttcc gatcgccgaa agagactacg
     5281 gcctcttcag cggcgtcatc gagctgatct acggccgggg ctttgggcaa cgctggcgcg
     5341 gcttcctcaa gctcttcggt cgcaagagct gatggaggag ttcgactaca tcgtcgtcgg
     5401 aggagggtca gcgggttgca tcgtcgcgtc tcgacttgcg gacgacgcgc gactgaagat
     5461 ccttcttctc gagagtgggc cgtccgctga agaacacccg gagactttgg ttgcggcggg
     5521 ctacaaggag gccttcatca acgacgcggt gatgggtgag cgattctcgg taccccaggt
     5581 gcactcggcc agacaacgag tctttgcagg caccggaacc gtcctcggag gaagcggctc
     5641 ggtgaacggg atggtctaca cccgcggcgc tcgcgaagac tatgcagagt ggccgaaagg
     5701 atggcagtgg gacgagattc aagatgagtt ccaagcaatc gagaagacac ttcgcccgca
     5761 tcagcggccg ccgacaaact ggaccgaagc ctgcatcgcc tcagccgagc aaaacggctt
     5821 cactcgcaag cgggatctca atgacggcga catgcacaac gcgatcggat acgagtggat
     5881 gtcctacgag ggtgaggaga gacgaagctc gtacgtggca ttcatgaagg atgtcggtcc
     5941 acgtccgaac ctcaccgtga tcaccggagc acacgttcac cggatcgtat tcaatgcggc
     6001 gaagcgggca cgcgccgtcg agttcgaaca cgagggcgcc ctcaagcgcg ccagcgccgt
     6061 tacggagatc attctcagcg ccggtgccct cgaaacgccc aagatcctga tgctctctgg
     6121 agtcggtccg agcgagcacc tgcgcgcatt cggcataccc gtggtcgcag accggccctc
     6181 cgtcggagag ggactacacg atcatcccaa cgtccccgtc ttcttcaaaa ccaaagccga
     6241 gattgattgc ttctaccccc aggtgtacag cttcttccgg acgaacgaag acagcgacct
     6301 gccaaaatcg cagagcgaca cctgctacgt ttactggcca gcaccatcgg cgatgcgcca
     6361 aatgatgcag cgcatgctcc caccgatggt cgtacccaat tcactctacg gcccacgctc
     6421 gaggcagatt gttcgcagcc tggtcgggac tgctttcaag atcggcgcca tccaacggtt
     6481 caccgaccaa atgttcgggg tcatcttgat tcttggaaaa ccgaagagcc gtggcacgct
     6541 tcggctcgag tccgctgacg cgcgcgccca agcgtccatc gaccccgctt attactcgca
     6601 cccggaggat ctacacacga tggtaaaggg agtgcgcatc gcgcgagcga tcggcagctc
     6661 caacgggctt gcggcctgga acgtgaaaga gctcatgcct ggcaagcgcg tcaatagtga
     6721 cgagagcatc gcgaattaca ttcgaagaaa caccatcacc acgtatcact ttgcaggcac
     6781 ctgcggcatg ggaacccttg aacgcgatgc cgtggacccc gagctccggc ttcgtggggt
     6841 ctctggcctg agaatcgcag atgcgtctgc gatcccgtgg acgccggtct ctgcgctcaa
     6901 tgcgccgagt atgctcatcg gctaccgcgc ttcagagctg atcctcaatg gggcaacgct
     6961 gagctaggca gtaggcagag actcgatgcc gcagtgaggc gattgctgct agtatctcgc
     7021 cttccccccg agacatccat gaacaaagcg aaacatgcct tcattgcact cctcgccgcg
     7081 agtgcctttt tccttcccgg tgcggtttcg gtgcccgctg cgaacgcggc ttcaccgatc
     7141 atcattcgct ttgcttcgct cgcgcctcct gggtcggcgt tcatgaagtt gatgcaagcc
     7201 tggaatcgaa gtctcaagaa ggaaaccgag aaccgggtcc agcttcggtt ctattccggc
     7261 ggaagtcagg gcgatgagcg ggacttcatc cgcaaagtgc gcgccgggca gatggatgca
     7321 gccggggtga cgacgaccgg cctcggtatg gttgcgcgcc cggtgctggt cttgaccgcg
     7381 cctggcatga tcaccgaata cgatcagctc gaacgcgcac gggcaaagct gagcgatcga
     7441 ttcgcgaagc tgttcgaaga agccgggttc gtgatgcttg cctggggtga ggctggaaag
     7501 aatcgaatct tctcgatgga ggagttcgcg aagccagaag atctcaagaa gctccgcccc
     7561 tgggcctgga aagacgaccc ggtctttgca ggctacatca gcacgatcgg cgccaacgcc
     7621 gtccgagtgg gcgtaccgga ggtgtacggc ggccttcaaa cccgcatgct cgacacggtg
     7681 cccgcatcgg ctctcgcagc ggtcgcgctc cagtggtaca cgcgtctcaa ctacatggcg
     7741 aagaccaact tcggcatcat catcggcggc tcactgatca agcaagagaa gttcgacgag
     7801 ctcacggagc acgatcagaa ggtgctgctc gacaccagcg agcgcgctgc gcgtgcgggc
     7861 gacgtgatgg cgcgccgcga cgacgaccga gcctatgcct cgctgctcaa gcgcggcatg
     7921 atcgaggtcg acacgaacga gtacaaggcc gagtgggatg cggtggcgaa gacgacacgg
     7981 gaaaacctca ccggccgtgt ctacagcaag agcctcctcg aagccgtaga agcggcaata
     8041 gcgccaaact agcgctccgc gccggacggc ttccgttcta tcccagatcg tcggcgagaa
     8101 acttcagcgt gagctcgttg aagagcttgg ggtactcgac catgacccaa tggccgcagc
     8161 tcgagatgag catcgtccgg gagctcgagc atcgagaggc aattttggcc gccccactcg
     8221 atgggcagaa tttgtcgtta gcgccccaaa aacagaggac cgggcagtgg atttccgaga
     8281 ggcggtcttc ttggttggtc acgtgaatgc gggcgatgtt gtctttgtgt tgcgtcatgg
     8341 caacttgata tcgctcttcg atgacttcat cggtgatctt cgtttcgtcg tgaagctgca
     8401 gcgcgaacac cctgcgcatc gtctctttgc tcacgccctc tttgtagagg actcgaatca
     8461 tcgccttgat gccctccatc gccatgtacg tctctcgagt ctcgaggccg ccgggcgcca
     8521 tcaagactag cttctgaacg agctgtggat agttcagcgc catgctgatc gcgatcgcac
     8581 cgccttgtga gttacccact acggtcactc gtgcgacgtc gagcgcgtcg agtaacgcct
     8641 tgtactgcgc ggcgacgtca tcgaggctga acgttccctc ctcaggttta gatgagtacc
     8701 cataccccat cgtgtcaggc acgatcgcgc ggtagccgtg ctcggcgagg attggatagt
     8761 tccccttgaa gttgctccag ccgctcgccc cagggccgct accgtggatg aacaggacgg
     8821 taccttgatc cccggtgccc gcctggtggt agtgcagagt gatgccgctc ccgacatcga
     8881 cgtattttcc ttcgggaacc gcttctacga ccatgtttca aacctcgaac tagaacgtgt
     8941 tctagctcac ccaaaccggc cggtccaatg gccgctcagt gcttcttttc gcccgcttcc
     9001 ttcgcccggc gataggcctt ggtgaggagt tgaggcggat ggtgccccca ttcgctgacc
     9061 ttgccgtgcg tcttcgtctc ccaggtctct tcgtcgattt caatggcacc cgcaccgaac
     9121 tcgaagtgaa acccggacgg agtgtacccg tagaacgaaa acatcttgtc gttcggatgc
     9181 cggcctagcg tttgatgcac cattccgcca gagaagagga aacggtcgta gcacattcca
     9241 acttcgtcga tcgatttcac ctggaccatg aagtggtgga tgcgcttcaa ctgggctcca
     9301 ccgaatgcca gcgagtgatg ccgcgcgttg gcatgcatga aggtcacgtc gatcttgtgc
     9361 ccgaagtact cgcagcggat gtagtcactg agcttcagac cgaacatcgt tgcgtagaag
     9421 gcctcatgct cgtccatgtc tttcgacacg acgacgacat ggccgaagcc ttgctcatca
     9481 gcgacgaacc ccgatcgaac gatatcggaa acgaatggtt cggccgccat ttgcgggctg
     9541 tgaaatacct cgaccggatt gcctgccggg tcggtgaacc gaatcaatgc ctcgacgttt
     9601 cgcttcgcag cttcttcgtc ggtgcactcg gtcacctcat gaccggcgct cctcagccga
     9661 tcgatcattc ggcccagctc ttcgggcccg cttacttccc agccgatcgc gacgaggtcg
     9721 tcggccggac cccgttcgac gaagaaacgt tggcgatagc catccattct gaggccaaat
     9781 ccggcgtccg ggcgctcgtc gaccagctgc aggccgagaa tctgcgtccc gaaacgctcc
     9841 caggcagcaa gatcgctcac ctcgaaaacc aaatatccaa gctgcttcac cgcactcatt
     9901 tgcctcgcct ccggcgggat catactacgg ggaacttctg aggctacgga gaaaagatcg
     9961 atggaaagca gcactaagct gagaggcccc acgcttccaa cagcggcggt catcggaatc
    10021 gcagtcgtgt tgggcacgtc gtttcacgtc tggctgcatc acggcgtgca cggtgtctac
    10081 aaccccaggc agatcggttt ggcgttcttc ttggtgatca acgtgttggt cgcgtggtgg
    10141 gaaatcgctc tcttcgtatg ccaagaccag attcggaccg aatacgatgc aactcaggag
    10201 ccctatcgag ggcgtgagat gacacgcatc gcggaagtct tcgcgaggcc gatcccgatc
    10261 ttccgtgtct tctgcttctg tgaatggagc aacatctggt cgagctactc gctattcgat
    10321 cccggctact cggaccgtcg ttccttcggc tataacatcg acgtcggaaa cgggttcacc
    10381 acgctgatac cggcgctgct ctttgcgttt ggaatgacgt tcgagctcat gccggcccgg
    10441 gttctcggca tcatgggagt gatcgtcttc tggcagatgt tctacggaac ggccgtctac
    10501 ttcttccagt tcttcaacaa ccgccgtcac cgaggacatt ccctcagaga cttactgctg
    10561 ttcgtcggac tgacgaacct gatgtggttc gtcttcccac tttgggggct cagcacgagc
    10621 attcagctga tcatggacgg ttcctacgct cttttcgttt agaccagtcg acgcgcccga
    10681 gccctcagca tcatccgttg catctgattg aagtctgcaa cggcgttggg gctcttaccg
    10741 gcgaaggggt acggtgcccc gatcgcactc gccgcgacca ggcctgacat gaaggacaaa
    10801 tcatgtccgc cttccggtgt ggtgaaggtc ccgcagtagt gaatgtcgcc cttcccctgc
    10861 agaaacttca tcagggacat gatgatgagg ttgcggagcg acaccgtgtg ggtcggatgt
    10921 gggagctcga tgcgcttgtg aacctcgccg atcgcctttt tcgaattgaa ggtgaccaac
    10981 atcggaacac cggcgtcttt cgtgctcggg ttttgggcgg aaatgacaaa agtacattcg
    11041 agctcgcccg catcgtcaat ctccgagtaa gtgttgtagt cggagagaat tcgcgcacgg
    11101 tgcttctccg gaaaaatgct cgtgtccgaa tggacgatga aacgcgaaaa cgtgggatcg
    11161 acgtcgtcga cgtattgcgc attgctgaag agagcctttt gtagccacgt cggcgactcg
    11221 agcgcgtcga gaacggagct cgcgtcgcag gcaaatacca acttgtcgac ttgataggtc
    11281 cggccctgca aatcccctat cgagaatccc ccgttacgac gtttgacgct ggtgatttcg
    11341 tgatcggtgt gcaccttgtc cgagaacggc gcagtcattc gttcgaagac ttgtcggggt
    11401 gcgccgcgcc aggttcccat ctgagtcggc tcttcgagcg gcacgatgct ttccagcaga
    11461 ggcaatatta cggcggggac gccacgcatg gaggtcgtga tgaagctagc gcagtggatc
    11521 ggaacgaaga tcgtgaccca gaaatcactc gaaactccga agagcctggc gagccagtag
    11581 agcggaatga ggttgagcgg attgaagtac gagaagtggt agagcgagtc gtgtctttcc
    11641 ttcggcagga agaaagagtt cactcggcta acgaacgcaa ccagctttcg ccagcgcctg
    11701 aaatcgtcct cgaatcgtcg atgcagctcg gtggcaccgt cctggcagaa gtacccatcg
    11761 ggggtctgta cgaagtacga gatcggaagc gtttcgatgc cgacgccgag ctcgtccaat
    11821 agcaactcgt agttgtggta gtacatctgc ggccaggcga cgacgagcag tggagactct
    11881 gcgtggcctt cacgggcggg ccagcgaaac gtcttcgcgt tgcccccgag cgcgggaccc
    11941 ttttcgaaga gctccacctc gaagccagcg cggtggagcg accaggcagc agcaacgccg
    12001 gcgataccgc cccccacgat tcctatcttc tcgctcattc ccaggcctcc aggagcctct
    12061 tgctaatggt tcttcggaac cagatcgacg atgggtccgt gcctcagcgt gtggcataag
    12121 cccttgtaaa tgccgagcga ctcgtagact gcagccgtca tcatgacagt ccgtgtctgc
    12181 ccgatgatcg acaccggaaa gctactgtga agaaaagcga acgccagccg gtgctcaggg
    12241 ttggcccatc ctcccgcgcc gccaaggccg aagtgtccga acgattcagg tacgtccagg
    12301 aggaacgcat cggctcgatg gtatccaagc ttccaatgaa gcggatagat tgtgactttg
    12361 tccgctcgtt tggtttgcac ttcggtcgcc ttcgcgatga tctccggaga gacgagccgc
    12421 acaccgtcga gctcgccgcc catggacagg acggcataca tcttcgcaag agagcgcgcc
    12481 gtcgcgactc cgttcatcgc cggcaacgag gctttccaaa atgcgggatg agaaaagagc
    12541 tgcttggcgt ttcgaggcgt gagtccgcgc cgacagaggt ctctcattcg cttcgaacgc
    12601 acccagaacg ggatgctgta gtcgatcgaa agccgctttg ccccacttcg ctgcagctgt
    12661 ggaagtccaa cgaaatcggg gatgcgatcc atttcggtgt ctgcattgcc gatgaatagc
    12721 ccatcgagcc ccaagggctc cacgatccgc tgggcgagga actccggaac ggtaagcccg
    12781 gaaatgcgcc taatgagctc gccgacgagc cacccgaacg tcatggcctg gtacgcgtta
    12841 gcggtgccag gagcgaaatc aggttcggtc ttctccaagc gtgaaacgat cagatcccag
    12901 tcgaggatgt cggtgagctt ctcgaccaga tgcacgatct tgtgcaagcc agcctgatga
    12961 gagagcagct gtcgaacggt aatccgttcc ttgcccttgc aaccgaactc cggccaatac
    13021 tcagcgacgg gcgcctcgta gtcgatgaga ccgtctgtcg cgagcatgtg gatcgcggtc
    13081 gagacgattc ctttcgtacc cgaatagcaa atcgtcatgg tgtcctgctt ccagggcgtg
    13141 ccatcgtgcc gcgcgggtcc ggcccatatg tcgacgacgg gcttgccatc gaggaacacc
    13201 gcagcagcag cgccaccacc atactggctg atctgcttgc gcatcagttt tgcgaccggt
    13261 tcgaatcgcg gctcgtacct gcctcggatc tctatcagct ctgccatcgg ttccgacctg
    13321 ggtagcgcac ctgggccact atgtcagggg cccgaagacg gacgctgcaa agtccaacag
    13381 gaacatcatg atcatgctcg cggagagcgc cgctgcgcag gtgatctgca ggctccaatt
    13441 ccggtctcga tcctcatcga gttggtaaaa gatggggaag ctggcgatga agtacagtga
    13501 atagaaaagc gaaccgtatc ggagcatacg gtccatgtcc ctgtagtaga actgcgcttc
    13561 gatccaagga ttggccatcg ccttggtctc catccaagcc cagaaatagg cgacggaaag
    13621 cagcagaatg ggccaaagca agggcccgat tggaagaccc gaagtgcgga tgcgccgaag
    13681 cacgattacc gctgtcgtat gataagtgag gaagtacgcc tgcgtgagca gatacatgat
    13741 gatcggaacc ctctgcacgc cggatccaac aagagccgcg tcgaggttga tctcgatcat
    13801 cgggtagtcg tagaccattc cgagaacgtc gaagaagtac tccgagccga agtagttcgc
    13861 agcgaaattg aagatcgcga cgtagagatt ggctttgaac caataggtct ggtaccagcg
    13921 gcggccgatt ggcctctcgt cacgaatcag ggccggaact acgagtaagg gaagcgcgac
    13981 agcgatgccg atggcgagga agcccagttc gccaattcgc ttggtagtcc cggatgccat
    14041 cacgacaccc atgaaagtca tccaaatcgg ggaatagacg aggaagaact tctcgcccca
    14101 ggctttgctg gggttcacgg agaaccaacg cgggctgtgc gcggtcgtca tgctgctcgg
    14161 cgtcgatagc gaattcgaat cggcggcttc ggccccagga ccaatccctg gaagtcggtt
    14221 tcgatcgggt cgcctacgag ctcgaaatcg aatcgattga gtaagactgc gatgatggtc
    14281 ttgatttgaa ggatggcgaa ggcatttccc atgcacttgt ggcgccctcc accgaatgag
    14341 atgtatgcga agggacgctt gtcttcctcg cggccagggc cgtagcggtc gggatcgtat
    14401 tcgagtgcat tcttgaacac gtcttcgtcg cgatgcgcga ccatcggcga aacggccacc
    14461 cagttgccct tcttgacccg gtaaccgagg atttcgcagt cttccagagc ggctcgaagg
    14521 aggatgaaca gcggtgggtg tatccgcaac gcctctttca cggcggcttc ggtcttctcg
    14581 aggtcacgaa gcgactggaa cgtgtagtct tcgtttgcgg aatagcttcg gcgaatctcc
    14641 ccacgcactt cctcaaggaa gcgtgggtga cggaggagct cgatgagggt ccatgcggtc
    14701 gtgacggagc tggtgtggtg acctgcgaac atcgctgcaa ccagcattcc cgtgatctcg
    14761 tggtcgctga gcttactgcc gtcggagtac tcggcatcca tcaaggtctg aaggaagtcc
    14821 tcaccgcggt atcccgactg tcttcgcttc tcgacgatct ccgagaccat gtgaccgagg
    14881 cgggcgcgcg ctctatcccg tcggcggaac gcaggaatgg gcaggtaggg atgaatgtac
    14941 ccccaggcca caatcccgcg ctcgaggtcg taatagacct tcgagaactc ctcgctcatc
    15001 tcgttgcgaa actccttgcc cagcaagcag ctgctcgagg tgaagttggt gagcatcccg
    15061 aaaaacgcgc tggcttcgat ttcgcccgag tcaccccaat cggccagaac atcgagcgtc
    15121 tctcgcgcta cgatctccgt atacgtgcgc attcgattgt tgcgaagcgc tgggtagagc
    15181 atgtgcatct gctccgccat tttcgcgggc tcggcgccat acgcgacgcc ctcgccgaaa
    15241 acgggaacca tcatcttgta agccgcgttc gggctcagaa cttcatcagg ctggcgaaag
    15301 agcgcttcat gcgcctcggg ccccgtgaag aggatcatgt ctttcccgaa aagcttgaac
    15361 cggccgacct tcccgcactc ttcgtaggtt cgctgaagta gtccgatcgt atccttgacg
    15421 aagtccatgg tatggccgat caaagggagg ccgcccttga gcttcggcgc ttgcttgagc
    15481 atcccaccgc caagaggtgc cgacgtagct tgtgctcgag ctgtcatgat ccactcctaa
    15541 ataggctccg ccgaagctgc cgttcgatct gttgtacgag gctcgtggcg ctgtcttcca
    15601 tgcctgtgtc gaaccacgaa gacatgaaaa cctgttgaat tgcagccatg agtcgcgccg
    15661 tgatgacggg atcagcttgg tagacgtctc cgtcgcgcat cgcctcttcg agcacgcgcg
    15721 ccatcagatc gatccctctg tgccaggcgg ccacgaggcc ttcaccgacc tgttgcggat
    15781 cggacgacca agctcccccg gcgtcgagat gaatgcgcag gtagtccggg tgttcggtca
    15841 accatcggac gaagacacga ttccccgcca acaaacgttc ggcagcgctc gcttcactct
    15901 tcatcgcgtg gccagtgagg cggaagagct cagccaagcg ctcgtcgtgg actgcctcgt
    15961 ggatgtcggc cttcccggcg aaaaccgaat acagagtgcc tagcgacaga ccagacgcgg
    16021 ccgcgatgtc ctggatcttc gtcttctcgt agccctgcgt cgcgaagagg tgctcggccg
    16081 agtcaatgac gagggaccga tacatcgacg ctttcgcact gcgggctcga gcaatggctt
    16141 ccgacacgat aaaaacagag tagaacatca ttactttttt tgtaacaata ttctacgttt
    16201 ggaactatat ggggaatagg aagggcattt ttcatgcgga tcaaggttga tttcgatctc
    16261 tgtcagggac acgcgaactg catgggcgag gcgcccgagg tattccaggt cgatgagcag
    16321 gggttcctga acatcctcca agaggaaccg cccgaagagc tccgggagaa agtcgagcta
    16381 gcggtaaagt actgccccac cggcgcgatc gggatggagg actgaccggg caccgagcga
    16441 ctagcgggag cggcatggtg gcccgccggg tggtgggcat tcggatacgg cgagcaggtc
    16501 catcgtgagc gcaacgccta cgtgaatgaa tacgcctccc cagatggagc gcgtccgcat
    16561 cgaaagcgtc ccgaggg
//
LOCUS       DASRZF010000162         8550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3893111, whole genome shotgun sequence.
ACCESSION   DASRZF010000162 DASRZF010000000
VERSION     DASRZF010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 8550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8550
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3893111"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..1280)
                     /locus_tag="VFG22_07370"
     CDS             complement(117..1280)
                     /locus_tag="VFG22_07370"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6416100.1"
                     /translation="MAIFMTGATGYLGSYATEWLLRNTDHELALLVRAPDYDAGVERL
                     WRSLQLHMKADEFHRHLDRVCFISGDLSKPGLGVSAADYDWLISNAESVLHNAAALNF
                     RSERTCTNHNLRGTLSVIKLARAIQEDHPLRRFTLISTAAVAGNRSGAIVREDEPLDW
                     SRSDFNPYTRTKKFCEHMVRELLPDVPCLVLRPSSVIGDSRFGETTQFDMVRALCALS
                     DLPVLPLSRDLRIDIVNADWVGHGIAELHLREDLAYDTYHLSAGAESRTVGEISEAFA
                     SALGKRVPRFAGPLAAPFEHAMDLLAKLPKTGNLQRIGALLSVFVPYLAADIVYDNTR
                     TVADLHTTPSPFTAYGPAVYKYAKSVNFEFPYEPLPENLDLSSVPPSPVRAMA"
     gene            complement(1287..2114)
                     /locus_tag="VFG22_07375"
     CDS             complement(1287..2114)
                     /locus_tag="VFG22_07375"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6416101.1"
                     /translation="MLAHSLIEPKTSDPAQYMLFLHGILGTRANWRAIARRFVEGRPK
                     WGAVLVDLREHGDSLGLAGPHSLSAAAADLVELEQALALPIGGALGHSFGGKVVLEWL
                     RSREGQATEAWTIDSSPSRGSANRDASATGEVLRTLETMPRTWASRDAFVRALTDSGQ
                     PEPLAQWLAMNLRRSEGGARHFGPDLGVIRELIDDYLRADCWDVVEALPLGTTLDLVI
                     AGRSSVFSRADRERVEALASTNPRISVHLIEQAGHWVHVDSADALLALLTSRAAPQG"
     gene            complement(2150..2851)
                     /locus_tag="VFG22_07380"
     CDS             complement(2150..2851)
                     /locus_tag="VFG22_07380"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET6416102.1"
                     /translation="MSGKVHASEAERRGAEAAELVAKAKAGDTHAFDELVRRYRARVY
                     SLALHLTGQASDADDITQDAFLKAYHKLSEFEGRSEFFTWIYRITLHRALNAKRDRKR
                     RRSVPLDDPRLVAAVAVDSHGDPERAAQLRDRYRALLEAFDRLSSLLQTTVVLTTLQG
                     LSYKEAAVVLETSEGTIAWRIHEARRKMQEYLEALETGSTRTKRRPITDSAVFRLEKA
                     LMQLVPTIPPSVAAT"
     gene            3108..4142
                     /locus_tag="VFG22_07385"
     CDS             3108..4142
                     /locus_tag="VFG22_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HET6416103.1"
                     /translation="MTDWFYGLFSNDLAIDLGTATTLIYVKGRGIVSCEPSVVAVQAD
                     PRGGKRVLAVGKEAKEMLGRTPGSIRAVRPLRDGVIADFDITEAMLRYFIARAHHNRR
                     TLVKPRIIICVPFGITEVEKRAVKESAEGAGAREVFLIEEPMAAAIGAGLPITEPSGN
                     MVVDIGGGTTEVAVISLAGIVYSQSVRVGGDKMDDAIIAYLKRKYNLLIGEQTAERVK
                     CTIGNAYPDGEASTMEVKGRDMVAGVPKTVIVNSDEIRDALTEPINAITEAVMMALER
                     TPPELSADIVDKGIVLTGGGALLKNLDVLLREETGLPVMVADDPVSAVVLGSGKALDH
                     LDLLREVTIA"
     gene            4165..5013
                     /gene="mreC"
                     /locus_tag="VFG22_07390"
     CDS             4165..5013
                     /gene="mreC"
                     /locus_tag="VFG22_07390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HET6416104.1"
                     /translation="MNYFQTIRDAAILLALLIIPAVFLRANLKNPSELSWLDRGLIQV
                     SGPVQHVSEWAADGVTSVIEDYVYLVDVKSDNDHLRAENDRLRREMRMLRTDAQRLKE
                     LESLLGLRERLATETVTARVIARDISPSFRVVRLAVDQGEQAGVRAGMPVVADEGLVG
                     QVRRVSGRFADVILTIDPESRVDVVVGESRARGRIEGLGERGRYRCRIQFDRADDQVD
                     VGDEVFTSGLGKKFPASILIGYVSSISDQEFGLQQEGEVTPSVDFTRLDEVLILTSGP
                     KDSEAL"
     gene            5021..5536
                     /locus_tag="VFG22_07395"
     CDS             5021..5536
                     /locus_tag="VFG22_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416105.1"
                     /translation="MRGARASIIVVLLAFAMLLTQSAFARVLAPYPFSPYLGLPLVFA
                     LGTAPGVRVLRGAATAFALGYLEDLFTGNPLGLHTFVFVVGYLVAWLVGYLLSFRGVV
                     FEMVLTFSLTVLLGGLLELIRSFTPGGMSWSGVSLTISLVASSFATALVAPILFALVR
                     LIDPESVRAPT"
     gene            5533..7395
                     /gene="mrdA"
                     /locus_tag="VFG22_07400"
     CDS             5533..7395
                     /gene="mrdA"
                     /locus_tag="VFG22_07400"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HET6416106.1"
                     /translation="MITLSQRREVGEFRKRYRWMALFVVLVFGGIIVRIAQVQLVQRD
                     RWSGIAQQNITKTLELPATRGVIRDIAGRVVASNRPSYDVYTTPRLLDPEAVQRVASL
                     MGLTKEERATLESRLAEVPQSRRSHQLKLYGDIDREQLAALETHLAELPALDIVTVPL
                     RDYPYATLGAHLIGYLNEVNSEDLAREPDASAYRSGDRIGRSGLEKHYEHLLKGTRGF
                     RRVVVDARGKALDGASPDSLEVEMLRKPVPGHEIQLTIDMELMEAFDRAFRPHPSGAA
                     VVVDIHTGAVRALYSKPVYDPNEMSSGLSTKRYAELTGDPFRPLIDKTIYETYFPGST
                     FKVITALAALEDAVVDPAQRVECLGSMKIGRQKFRCNKRHDWVDLYEAIVQSCNVYFW
                     ALAEEVGLERINSYARAFGFGERTGIGVNSEASGFLATREWYTEHYGAFRIGYTLNTA
                     IGQGNTRVTVLQLALANAAIANGGTLYVPQVVQNVTAPDGSLSDTFQPRIKREVGVSA
                     EHLDMMRRALIDVVEDESGTAYRARNKDGIRVAGKTGTAQVVSRKGRTEESSSAWYLD
                     RSHGWFAGFAPADDPQVAFAVLVEHGGSGGASAAPIATSVMQQYFSHPEPTEAE"
     gene            7398..8507
                     /gene="rodA"
                     /locus_tag="VFG22_07405"
     CDS             7398..8507
                     /gene="rodA"
                     /locus_tag="VFG22_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HET6416107.1"
                     /translation="MAIGRTIRDDFDWPLFIAVSTIALFGVLNLYSATDGGTSSDAYV
                     QQLYWLILGFGVAGLTVAIDYRQFERYGWVIYGACTTALVLVFVLGRSVRGSTRWISL
                     GGFSLQPSELMKIGLIIALAKYLHHDTKTGGRTLVDLIIPGLILALPMSLVLLQPDLG
                     TAMVLAFIFASVMMLTQLKMRSVVTLLASFVVAAPVTWFYLLKDYQKERVTAFWDRLD
                     GTQTDLLDSGWHTHQSLVAIGSGGLTGKGFTQGTQTRFRFLPDQHTDFPFPVWAEEHG
                     FLGALILICLYLFLVLWSLRIASQAKDRFGAVVAVGVGAMIFWQTVVNLGMVSGLLPV
                     VGMTLPLFSYGGSSLLSTMLGLGLLMNVSMRRFSH"
BASE COUNT         1531 a         2589 c         2688 g         1742 t
ORIGIN      
        1 tctccgccct tgcggaagat gccccattcg gtgcgcaccc agtcgacggt gttcttcagc
       61 cccatcctcg gcccttctac caccccgacg ggcgcatgaa aagcgcctga ccttgctcag
      121 gccatcgcac gcacgggact cggaggcacg ctcgaaagat cgaggttttc cggcaggggc
      181 tcatatggga actcgaaatt cactgatttt gcatatttgt ataccgccgg gccgtacgcg
      241 gtgaacggac tcggcgttgt gtgcagatcg gcgacagttc gtgtgttgtc gtagacgatg
      301 tccgccgcca agtaggggac gaacacgctg agcagcgcac cgatacgctg caggtttccc
      361 gtcttcggga gctttgccag tagatccatc gcgtgctcga acggcgcagc gagaggcccg
      421 gcaaaccggg ggactcgttt gccgagggcg gatgcaaagg cttccgaaat ctcaccaacg
      481 gttcgcgact cggcaccggc actgagatgg taggtgtcat acgcgaggtc ttcccggaga
      541 tgcaattccg cgatcccatg cccgacccag tctgcgttca cgatgtcgat tcgaagatcg
      601 cgcgaaagtg gcaacaccgg aaggtccgaa agcgcacata gtgctcgcac catgtcgaac
      661 tgggtggttt cgccgaagcg tgagtcaccg atcacgctgc tcgggcgaag caccagacat
      721 ggtacgtcgg ggagtaactc gcgtaccatg tgctcgcaga acttcttcgt tcgtgtgtaa
      781 gggttgaagt ccgacctgct ccaatcgagt ggctcgtctt ccctcacgat ggcgccgctg
      841 cgattccccg cgacggccgc tgtggagatg agcgtaaagc ggcgcagtgg atggtcttct
      901 tggatcgcgc gcgccagctt gatcaccgac aaggtgccgc ggaggttgtg attcgtgcag
      961 gttcgctcgg agcgaaaatt gagcgcggcg gcgttgtgga gcaccgattc cgcgttcgag
     1021 atcagccagt cgtagtctgc cgccgagact ccgaggccag gcttgctgag gtcaccgctg
     1081 atgaagcaaa cccgatccag gtgacgatgg aactcgtcgg ccttcatgtg cagctgcagt
     1141 gaccgccaga ggcgctcgac ccctgcatcg tagtcgggag cgcgaaccag caacgcgagc
     1201 tcgtggtccg tgttacgaag gagccattca gtcgcgtacg agccgagata gcccgtcgcc
     1261 ccggtcatga agatcgccat cgaagcctag ccttgtgggg ctgcccgaga agtcaagagc
     1321 gccagcagtg cgtccgccga gtccacgtgc acccaatgcc cggcctgctc gatcaggtgc
     1381 accgaaattc gggggttcgt cgaggccagt gcttcgacgc gctcacgatc cgcgcgcgag
     1441 aacaccgaag aacggccggc gatgaccagg tcgagggtcg tgcccagggg tagggcttcc
     1501 acgacatccc agcagtcggc ccgcagatag tcatcgatca gctcgcggat caccccgagg
     1561 tccggaccaa agtggcgagc accgccttcg cttcggcgaa ggttcatggc aagccattgc
     1621 gcgagcggct caggctgccc tgagtcggtc agtgcgcgga cgaacgcgtc tcgcgatgcc
     1681 catgtcctgg gcatggtttc gagtgtgcga aggacctcgc ccgtagcgga cgcatcgcga
     1741 ttcgcgctgc cccgggatgg ggaagagtcg atggtccagg cttcggtcgc ttggccttcg
     1801 cgcgaacgca accattcgag tacgaccttg ccgccaaagc tatgccctag cgccccgccg
     1861 atgggcaagg cgagtgcctg ctcgagctcg actaggtctg cggctgcggc cgaaaggctg
     1921 tggggaccgg ccagcccaag ggaatctcca tgctcccgga ggtcgacgag gaccgctccc
     1981 catttcggtc ttccctcgac gaaacgcctc gcaatggctc gccaattggc ccgggtgccc
     2041 agaataccgt gcaagaacag catgtactgc gccgggtcgg aggtcttggg ttcgatcaag
     2101 ctgtgggcaa gcacgcgctt ggcttaccac agatgtggca agggcccgat caggtcgccg
     2161 caaccgaggg cggaatcgtc ggcacgagct gcatcagggc cttctctagc cggaacaccg
     2221 cactgtctgt gatcggccgc cgcttcgtgc gggtcgaacc cgtttcgagg gcctcgagat
     2281 actcttgcat cttccgtcgc gcctcgtgga tgcgccaggc gatggtccct tcgctcgttt
     2341 cgaggaccac agccgcctcc ttgtagctga gaccttgcaa ggtcgtcagc acgaccgtcg
     2401 tctgcagaag cgatgacaag cgatcgaagg cctccagtag ggcacggtat cgatctcgca
     2461 actgagcggc gcgctccggg tctccgtgcg aatcgaccgc aacggctgcg accaggcggg
     2521 ggtcgtccag tgggacgcta cgcctgcgct ttcgatcgcg ctttgcgttg agagcgcggt
     2581 ggagcgtgat ccgatagatc caggtgaaaa actcgcttct cccttcgaac tcggaaagct
     2641 tgtggtaggc cttgagaaat gcgtcctgcg tgatgtcgtc cgcatcgctg gcctgcccgg
     2701 tcaggtgcag cgcgagtgag tacacgcgcg cccggtaccg gcgcacgagc tcgtcgaatg
     2761 cgtgggtgtc tccggccttg gcctttgcga cgagctccgc ggcctccgcg ccacgccgct
     2821 ccgcctccga tgcatgtact ttcccgctca acaggccccc atctgttcag gacccctaaa
     2881 ggctactcag tttcctggcc catacccaaa tcgctgggtc atcatgcgct gtaggggctt
     2941 gtgggatagc cgtgggctat ccgccatggc ctcgcttgaa acgcctaatc caagctgtta
     3001 catgcaaccc gcctgcgcgc ggaacaccct ggaaatggtg gggaattctc tctagcccgc
     3061 gctggatctc atctgctttt cgcctcgggc ccaagctcac acccatgatc accgattggt
     3121 tctacggcct cttctcaaac gacctcgcca tcgacttggg cacggcgacg accctcatct
     3181 acgtgaaggg ccgcggcatc gtgtcctgcg agccctccgt ggtcgcggtc caggcggacc
     3241 cgcgaggcgg taagcgcgtt ctggcggtcg gcaaagaagc caaggaaatg ctcggtcgca
     3301 cgccaggttc gattcgagcg gtgcgaccac ttcgcgacgg ggttatcgcc gacttcgaca
     3361 tcactgaagc gatgcttcgc tacttcatcg cgcgggcgca ccacaaccgc cgtacactcg
     3421 tgaagccccg gatcatcatc tgtgtgccct ttggcatcac cgaggtcgag aagcgtgccg
     3481 taaaggagag cgctgaaggt gccggtgcgc gcgaagtgtt tctgatcgaa gagccgatgg
     3541 cagcagcgat cggtgccggc ctgccaatca ccgagccgag tggcaacatg gtcgtcgaca
     3601 tcggtggcgg tacgacggag gttgcagtca tttcgctggc ggggatcgtc tactcgcaat
     3661 cggttcgtgt cggcggcgac aaaatggatg acgcgatcat cgcgtacctc aaacgcaagt
     3721 acaatcttct gatcggcgag cagaccgccg agcgagtcaa gtgcaccatc ggaaacgctt
     3781 acccagatgg cgaggcgtcg acgatggaag tcaagggtcg cgacatggtg gccggagtcc
     3841 ccaagaccgt catcgtaaac tccgacgaga ttcgcgatgc gctcactgaa ccgatcaacg
     3901 cgatcaccga ggcggtgatg atggcactcg agcgaacccc gcccgagctt tccgccgaca
     3961 tcgtcgacaa gggcatcgtg ctcaccggtg gcggggcact cctgaagaat ctggacgtgc
     4021 ttttgcgcga ggaaacaggg cttccggtga tggttgcgga tgatccggtg tccgctgtcg
     4081 tgctcggttc cggcaaggcg ctcgaccatc tggatctgct ccgtgaggtg acgatcgcgt
     4141 agtgcgaggg gcaagaacgc ggcgatgaac tatttccaga cgatccgaga tgccgccatc
     4201 ttgcttgcat tgctgatcat ccccgccgtt ttcttgcgcg cgaatctcaa aaacccgtcg
     4261 gagctttcct ggctcgaccg cggcttgatc caagtgagcg gtccggttca gcacgtttca
     4321 gagtgggcag cggacggagt gacgtcggtg atcgaagact acgtgtactt ggtcgatgtc
     4381 aagagcgaca atgaccattt gcgtgcggag aacgatcgct tgcggcgtga gatgcgcatg
     4441 ctgcgtaccg atgcccagcg cttgaaggag ctcgaatcgc tactgggcct tcgcgagcgt
     4501 ttggcgaccg agaccgttac ggctcgcgtg atcgccaggg acatctcgcc gtcgtttcgc
     4561 gtcgtgcgac tcgcggtcga tcagggcgag caagcgggtg tgcgcgccgg catgcccgtc
     4621 gtcgccgacg aagggctcgt cggtcaggtt cgacgcgtct cggggcgctt tgccgacgtc
     4681 atcctcacga tcgatcctga gagccgggtc gatgtcgtcg ttggcgaaag cagggcgcgc
     4741 gggcgcatcg agggcctcgg cgagcggggc cgttatcgct gtcgcattca gttcgatcgc
     4801 gctgacgatc aagtggacgt tggcgacgag gtgttcactt cgggcctcgg caagaaattc
     4861 cctgcgtcca ttctcatcgg atatgtcagc agcatcagtg accaggagtt tggattgcag
     4921 caggaaggcg aagtgactcc gtcggtcgac ttcacgagac tggacgaggt gctcatcctc
     4981 acctccgggc ccaaggactc ggaggcgctc tagggcaggt atgcggggcg cgcgagccag
     5041 catcatcgtc gttttgctcg cgttcgcgat gctgctgact cagtctgcgt tcgcgcgggt
     5101 gctggctccg tatccgtttt cgccttacct ggggctcccc ttggtcttcg cgctgggtac
     5161 cgctccgggg gtgcgggttt tgcgcggcgc cgcgacggct tttgcgctcg gctatctaga
     5221 ggacctcttc actgggaatc ccctcgggct gcatactttc gtattcgtcg ttggctatct
     5281 ggtggcttgg ttggttgggt acctcctgtc gtttaggggc gtggtgttcg agatggtgct
     5341 cacgttttca ctcacggtgc ttctcggtgg cttgctcgag ctgattcgga gtttcacgcc
     5401 aggaggcatg agctggagcg gagtcagcct cacgatttca cttgttgctt catccttcgc
     5461 gaccgcgcta gtggcgccca ttctcttcgc gttggttcgt ttgatcgacc ccgagtccgt
     5521 gcgagcgccg acgtgatcac tctctctcag cgcagagagg tcggtgaatt ccgcaagcgg
     5581 tatcgctgga tggcgctttt cgtggtgctc gtcttcggag gaatcatcgt gcgcatagcg
     5641 caggttcaac tcgttcagcg cgatcgctgg tcgggtatag cgcaacagaa catcacgaag
     5701 acgctcgagc tgcccgccac ccgcggggtc attcgggaca ttgccggtcg cgtcgtcgcc
     5761 tcgaaccgcc cgtcctacga cgtttacacg accccacgac ttctcgaccc cgaagccgtg
     5821 cagcgcgtcg cttcgttgat gggtcttacc aaggaagagc gagcgaccct cgaatcgaga
     5881 ttggctgaag tgcctcagag ccgtcgcagt catcaactca agctctatgg tgacatcgat
     5941 cgagagcagc ttgccgctct ggagactcac ttggccgagc ttcccgctct ggacatcgtg
     6001 acagttcccc tccgagatta cccctacgcg actctgggcg cccatttgat cggatacttg
     6061 aatgaagtga actccgagga tctcgcccgc gagcctgacg cgagcgccta tcggtccggc
     6121 gaccgcatcg gtcgaagcgg cctcgaaaag cactacgagc atctgctcaa gggcacgcgc
     6181 ggattccgcc gtgtcgtcgt cgatgcgcgc ggcaaggcgc tcgacggcgc cagtccggat
     6241 tcgctcgaag tggagatgct gaggaagccc gtcccgggtc acgaaatcca gctcactatc
     6301 gatatggagt tgatggaggc attcgaccgg gcgtttcgcc cgcacccctc cggcgccgcc
     6361 gtcgtcgtcg acattcacac gggggccgtt cgcgctctct attcaaaacc ggtctacgac
     6421 cccaacgaaa tgagcagcgg tctcagcacg aagcgctacg cagagctcac gggtgacccg
     6481 tttcgacctc tgatcgacaa gacgatctat gaaacctatt tcccagggtc gacgttcaaa
     6541 gtcatcacgg ccctcgcagc actcgaagac gcggtcgtcg atcctgcaca gagggtcgaa
     6601 tgcctgggat cgatgaagat tgggcggcag aagttccgct gcaacaagcg ccacgactgg
     6661 gtcgacctct acgaggcgat cgtgcagtcc tgcaacgtct acttctgggc gctggctgaa
     6721 gaggttggcc tcgagcgcat caacagctac gccagggcct tcggatttgg cgagcgcacc
     6781 gggatcggcg tgaacagcga agccagtgga tttctggcaa cgcgcgagtg gtacaccgag
     6841 cactacgggg cgtttcgcat cggctacacg ctcaataccg cgataggcca gggcaacact
     6901 cgggtgacgg tgcttcagct cgccttggcg aacgcggcaa tcgcgaatgg agggactctc
     6961 tacgttccgc aggtggtaca aaacgtgaca gcgcccgacg gttccctgtc ggacactttc
     7021 cagccgcgca tcaaacgaga agtcggtgtt tctgccgaac acctcgatat gatgcggcga
     7081 gcgctgatcg acgtggtcga ggacgagtcc ggaacggcat acagggcgcg gaacaaggat
     7141 ggaattcgtg tcgccggcaa gaccgggact gcgcaggtcg tcagccgtaa ggggcgcacc
     7201 gaggaatcct cgagtgcctg gtacctggac cgttcgcacg gttggtttgc tggctttgca
     7261 ccggctgacg atccgcaagt ggcctttgcg gtcttggtcg agcacggtgg ctccggcggg
     7321 gcgagcgctg cccccattgc aacctccgtg atgcagcagt acttcagtca cccagagccc
     7381 acggaagcag agtagccatg gcaatcggca ggaccatccg agacgatttt gattggccgt
     7441 tgttcatcgc ggtctcgacc atcgcgctct tcggcgtgct caacctctac agcgcgaccg
     7501 atggcggcac cagctcggac gcctacgtgc agcaactcta ctggctcatt ctcggctttg
     7561 gtgtcgctgg tttgacggtt gccatcgact accggcagtt cgaacggtac gggtgggtca
     7621 tctacggggc ttgcactacc gctctcgtgc tggtgttcgt gctcggccgg agcgttcgcg
     7681 gttccacgcg gtggatttcg cttggcggtt tttccttaca gcccagcgag ctgatgaaga
     7741 tcggcctgat catcgcgctt gccaagtacc tacatcacga taccaagacc ggtgggcgca
     7801 ctctcgtcga cctgatcatc ccagggctca tactggccct tcccatgagc ctggtccttc
     7861 ttcagccgga ccttggcacg gcgatggttc tcgcgttcat ctttgccagc gtgatgatgc
     7921 tgacgcagtt gaagatgcgc tccgtcgtca ccctgctggc cagcttcgtg gtcgccgcac
     7981 cggtgacctg gttctatctt ctgaaggact accagaagga gcgagtcact gcgttttggg
     8041 accgattgga cgggactcag acggacctct tggattccgg atggcacaca caccagtcgc
     8101 tggttgcgat agggagtgga gggctgaccg gcaagggatt cacccagggc acccagaccc
     8161 gctttcgatt ccttcccgac caacacaccg actttccctt tccggtctgg gcggaggagc
     8221 atgggtttct gggcgccctg atcctgatct gcctctactt gttcctcgtt ctctggtccc
     8281 tccggatcgc gtcccaggcg aaggaccgtt tcggcgcggt tgtggcggtc ggtgttggcg
     8341 ccatgatctt ctggcaaacg gtggtcaatc ttgggatggt ctcggggttg ctgcccgtcg
     8401 tcgggatgac gcttccgctg ttttcctacg gtgggtcgag cttgctctcg accatgttgg
     8461 gcctgggctt gttgatgaac gtgtcgatga ggaggttttc acattgagcg agtcgaagcg
     8521 agtgatggca ggagtcgtgc agatgacctc
//
LOCUS       DASRZF010000163         3955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_797987, whole genome shotgun sequence.
ACCESSION   DASRZF010000163 DASRZF010000000
VERSION     DASRZF010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797987"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..314
                     /locus_tag="VFG22_07410"
     CDS             165..314
                     /locus_tag="VFG22_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416108.1"
                     /translation="MISTVSFIQIRRLDGGSTGKLGFSLRGLVKVFSKWIDVNHEVES
                     IRLRK"
     gene            complement(281..2959)
                     /locus_tag="VFG22_07415"
     CDS             complement(281..2959)
                     /locus_tag="VFG22_07415"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HET6416109.1"
                     /translation="MSQIHEVAPERVFESLQSRSEGLTADEAAERLRELGKNTLGAPV
                     RFRLIRSLFRQLTNFFSLLLDVAAGLCFVADAMQPGERMNVLGWALLGVSLLNALFAF
                     FQEYRAERAMQALAQFLPPRLVLRRGGRELDMLAEDVVPGDVLLIREGDRISADARLV
                     ECNDLLVNNAPLTGESAAAALTEAAESGRLIDSSNIVFAGCSVLKGSGVAVVFATGER
                     TQFGKVAALSRDIRRPASPLERQTTHMVRVLTAIAVTMGVGFFTYGVFAGRPLWVNLV
                     FMLGIIVANVPEGLLPTFTLSLAMGSLRMARKNVLVKSLNAVEALGAVHAICTDKTGT
                     LTLNELSIARMVDARASEIPPSDPRRANLLLSGLIASEVRRQADKWSGDPLDVALADM
                     FETEVGDVDGVLRGVTRRFSFDVRKRRSAGVWHDGGESVVAVKGAWEALRPLVAEIAE
                     PDGTARSATEELLTETDALVDELAGEGQRIIAIATRGLSVGAEDAEQDQLEHGLTLLG
                     FLCLEDPLRAEVPEAVNQCHQAGIRVLLVTGDHPRTAFAVARKAQILPASASSPEDGL
                     TLLVTGDELERLRQTELCDLLENGACVFARTTPEQKMKIVTGLKRLGLVVAMTGDGVN
                     DAPALKAADVGVAMGVGGTDVAREAAQIILLDDNFASIVAGVAEGRTIFDNIGKFTNY
                     VLVSNGPEILPYLIYILFPVPLALTIIQILAIDLGTDIIPSMALGQEPTDQDTMKRPP
                     RRRDAPLLSFRLVAHSYFFLGLIEAAYSLFLFFWVLHSGGWRYGEELAAADPLYRSAT
                     GLALATIMLMQIGNLVGRRSATRSGLDRGLLTNHLMLIGVALEILFSWGALYFPPLCA
                     VLGTGPVSLQMYAMAWLGAPLIFMADYLRKRMLSTS"
     gene            complement(2956..3795)
                     /locus_tag="VFG22_07420"
     CDS             complement(2956..3795)
                     /locus_tag="VFG22_07420"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET6416110.1"
                     /translation="MLHFAYDGSIHGDWVARYAIQMATRLPEPRLRVLHVRDGQVSDA
                     ALQRGLARIESECASLSVPCELAVHPARGTVAATLLGLVPQSPDQYLVCGTRARHKGR
                     GFLTGTVSQQLLREGGCQVLSLRVIQPGLLGVPRSFLVPVSGRPEGIQVGLPFLRLLV
                     PESAHLEVLMVRRMSRQRFRDLSQDEAARLIERGQVYVLGIERSLSTELQLPFGRLDA
                     AVVLSDDLPKEIVIQANKAKSQIIYMGASAVSLRQRSVYGSPIEQVLRNAPCDIAIYG
                     TPS"
BASE COUNT          792 a         1235 c         1243 g          685 t
ORIGIN      
        1 tcctcggcta ctgagcccga cctgcgtgga cgatcggatt gccctcggtg tcgcggctga
       61 tgtggagggc ccacggtcgg cagcacacgt cacagtcctg gattagctcg ccctcgttct
      121 cagggtcgac gtagacctcc aggcgttcga aacaataggg acaagtgatt tcaaccgtgt
      181 cgttcatcca gattcgccgc ttggacggag gctcgacggg caagcttggg ttctcgctcc
      241 gaggcctcgt caaggtgttc tcaaaatgga tcgacgtcaa tcacgaggtc gagagcatcc
      301 gtttgcgcaa gtagtcagcc atgaatatca gcggcgcgcc gagccaggcc atcgcgtaca
      361 tctgcaagga caccggtcca gtgccgagga cagcgcagag cggagggaag tagagcgcgc
      421 cccaagagaa cagtatttcg agcgcgacgc cgatgagcat gaggtggttc gtgagaagtc
      481 cgcgatccaa gcccgaacgg gtggcggaac ggcggccgac cagattgccg atctgcatga
      541 gcatgatcgt ggcaagcgcg agcccggtcg cagatcggta caatgggtcc gcagccgcga
      601 gctcctctcc gtagcgccag cctccggagt ggaggaccca gaagaagagg aagagcgaat
      661 acgcagcctc gatcagtccg aggaagaagt aactgtgagc gaccaaccgg aagctgagca
      721 gaggcgcatc gcgccttcgg ggcggccgct tcatggtgtc ctggtccgtc ggttcttggc
      781 cgagcgccat cgaaggaatg atgtcggtac cgaggtcgat cgcgaggatc tgaatgatcg
      841 tcagtgccag cggcaccgga aagaggatgt agatcaggta cggcaggatc tccggtccat
      901 tgctgacgag cacgtagttg gtgaacttcc cgatgttgtc gaagatcgtc cgtccttctg
      961 cgactcccgc gacgatcgat gcgaagttgt catcgagcaa gatgatctga gcggcctcgc
     1021 gtgcgacgtc tgtgcctcca acacccatcg ctacgccgac atccgctgct ttgagggccg
     1081 gcgcgtcgtt gacgccgtcg ccagtcattg caaccaccag gccgaggcgc ttcagcccgg
     1141 tgacgatctt cattttctgt tcaggcgtcg tccgcgcgaa aacacaagcg ccgttctcga
     1201 ggaggtcgca gagctcggtt tgccgtaagc gctccaactc gtcgccggta acgagaagcg
     1261 ttagcccgtc ttcgggagac gaggcggatg ctggcaggat ctgggccttg cgcgccaccg
     1321 caaaggccgt ccggggatgg tcaccggtga cgagcagaac tcgaatgccc gcttgatggc
     1381 attggttcac ggcttcaggg acttctgcgc gcagcggatc ctcgaggcat aagaaaccaa
     1441 gtagcgttag cccgtgttcg agctggtctt gctccgcgtc ctcggcgccg accgaaagac
     1501 cgcgtgtcgc gatcgcaatg atgcgctgac cctcaccggc tagttcatcg acgagcgcat
     1561 cggtttccgt gagcaactcc tctgtcgcgc ttcgggccgt cccgtccggc tccgcgatct
     1621 cggcgaccag cggacggagc gcctcccagg cacccttgac cgcaacgacc gactcccctc
     1681 cgtcatgcca aaccccggcc gaccgtcgtt tgcggacgtc gaacgagaat cggcgcgtga
     1741 ccccccgaag aactccatcg acgtcgccga cttcggtctc gaacatgtcc gcaagggcaa
     1801 cgtcgagcgg atcccccgac catttgtccg cttgccggcg gacttcactt gcaatcagac
     1861 ccgagaggag caagttcgcg cgccttgggt ccgaaggcgg aatttcgctg gctctcgcat
     1921 cgaccattcg cgcgatcgac agctcgttga gggtgagcgt gccggtcttg tcggtgcaga
     1981 tcgcatgcac cgcaccgaga gcctcgaccg cgttgaggct cttgacgagc acgttcttgc
     2041 gcgccatccg caagcttccc atcgccagcg aaagagtgaa cgtgggaagc aagccctccg
     2101 gaacgttggc cacgatgatg ccgagcatga agacgagatt cacccagagg gggcggcccg
     2161 cgaagacgcc gtaggtgaag aagcccacac ccatcgtgac cgcgatcgcc gtcaggacgc
     2221 ggaccatgtg cgtcgtttgg cgctcgagag gggaagcggg ccgtcggatg tcccgggaaa
     2281 gcgccgccac tttgccgaat tgcgttcgtt cgccggttgc gaacacgacc gcgacgccgg
     2341 aacccttcag aacggaacaa cccgcgaaga cgatgttgct gctatcgatc aggcgcccgg
     2401 actcggcggc ttccgtgagc gccgcagcag cggattcacc ggtgagcggc gcgttgttga
     2461 cgaggaggtc gttgcactcg accagccgtg catctgccga aatccgatcg ccttccctga
     2521 tcagaagcac gtctccaggg acgacgtctt ctgccaacat gtcgagctcg cgacccccgc
     2581 ggcgcaacac cagacgaggc ggcaagaact gcgctaaagc ctgcatggca cgctcggctc
     2641 ggtactcttg gaagaatgca aacaaggcgt tgagcagcga gacgccgagc agggcccacc
     2701 cgaggacgtt cattcgctcg ccaggctgca tcgcatcggc gacgaaacag agtcctgcag
     2761 ccacgtcgag caggaggctg aagaagttgg tcagttgccg gaaaaggctt cggatcaggc
     2821 gaaaccgaac gggagcgccc aaggtgttct tgccgagctc gcgcagacgt tccgcagcct
     2881 cgtccgccgt caggccttcg gaacggctct gtagcgattc gaacacccgc tccggtgcga
     2941 cttcgtggat ctgactcacg acggcgtacc gtagatggcg atgtcacacg gcgcgtttcg
     3001 caagacctgc tcgatcggac tgccgtacac cgagcgctga cgaagcgaca cggcagacgc
     3061 acccatgtag atgatctgag acttggcctt gttcgcctgg atgacaattt ctttgggcag
     3121 gtcgtcggag aggacgacgg ctgcgtcgag ccggccgaat ggaagctgta gctcagtgct
     3181 gagcgaccgc tcgatgccga gaacgtagac ctgtcctcgc tcgatcaagc gcgctgcctc
     3241 gtcctggctg aggtcccgga accgctgcct gctcatccgg cgcaccatga ggacttccaa
     3301 atgtgcagat tcgggcacca acaagcgaag aaagggcaat ccgacctgaa tgccttccgg
     3361 ccggccgctt accggaacca ggaagctgcg aggcaccccc agaagccccg gctggatgac
     3421 tcggagtgat aggacctgac agcccccttc gcgaagcaac tgttgcgaca ccgttcccgt
     3481 cagaaaaccg cggcccttgt gacgggcgcg ggttccgcaa acgagatact gatcgggact
     3541 ctgcgggacg agcccaagga gcgtcgccgc caccgtgccg cgggccgggt gcaccgcgag
     3601 ctcgcacggg accgacagtg atgcgcattc ggactcgatc cgcgcaagac ctctctggag
     3661 agccgcgtcc gagacttgcc catcgcgaac gtgcagcaca cgcagcctcg gctccggaag
     3721 tcgcgttgcc atctggatcg catagcgagc aacccagtcg ccgtgaatcg acccgtcgta
     3781 ggcgaagtgg agcatgactg acccgagggg acccacggtc accccgaatt ggaaagagct
     3841 tttgtcgaga gccttaccag agcgctctag tcgctgcaat tgaggcctcg taacgactct
     3901 gaggcgtctg aggcgcactc gcctctaggc aagtctcctg ctaggttgcc tgcga
//
LOCUS       DASRZF010000164         9890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1484680, whole genome shotgun sequence.
ACCESSION   DASRZF010000164 DASRZF010000000
VERSION     DASRZF010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 9890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9890
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1484680"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /gene="pip"
                     /locus_tag="VFG22_07425"
     CDS             complement(<1..826)
                     /gene="pip"
                     /locus_tag="VFG22_07425"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HET6416111.1"
                     /translation="MAELFPEIEPYDSGHLRVDSIHSIYYEQCGNPEGKPAIFVHGGP
                     GGGSSPVHRRFWDPSKYRIILFDQRGCGRSTPHAELQNNTTWHLVDDIERIRVHLGIE
                     RWQLFGGSWGSTLSLAYAQAHPERVTELILRGIFLLRRREIDWYYQEGASRIFPEAWQ
                     KYLEPIPEEERGDMLSAYHRRLTSSDREERIRAARAWSMWEGSTSRLIPDPVLIERTG
                     DVTFAEAFARIECHYFINAGFLEEDDRLLRQVGIIRHIPAIIVQGRYDIVCPAESAY"
     gene            897..2489
                     /locus_tag="VFG22_07430"
     CDS             897..2489
                     /locus_tag="VFG22_07430"
                     /inference="COORDINATES: protein motif:HMM:NF001964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HET6416112.1"
                     /translation="MGQNEYQRRRTFAIISHPDAGKTTLTEKLLLYGGAIHAAGAVKS
                     RKAKRSAVSDWMALEKQRGISVTTSVLQFEYDEVRWNLLDTPGHNDFSEDTYRTLMAA
                     DCAIMILDAARGVEPQTRKLFHVCRMRNTPVVTFINKMDRPARDAFALMSEVEEVLGV
                     STVPYTWPIGSGDLFQGVYDRINKLVLRFERSSEGQAKRAEMKVTGLDDPELDTLLGA
                     EAAQTLRDEVELLDGAGEQWDEDRFRAGEITPVFFGSALTNFGVEPFLRAFKGLCPPP
                     GKREASGDVVVSPDDDRFSAFVFKVQANMDPSHRDRIAFARICSGKFEGGMRVHHYRL
                     GKEIRLNRAEQFFAQERESVLEAYAGDIVGLYDPGLFRIGDTLSTNGPMSFDAVPRFS
                     PEHFGRLQLLDPLRRKQLLKGIQELSEEGTVQLFLEPGREKDPVCGVVGRLQFDVLMF
                     RLEHEYGAKVTFDLLPYQHARWVEGAADCTELTAKRVPMAVLDRDDRPVALFRDEWEL
                     GRAERDNENWTFHETAPIFVAA"
     gene            2552..3151
                     /locus_tag="VFG22_07435"
     CDS             2552..3151
                     /locus_tag="VFG22_07435"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HET6416113.1"
                     /translation="MKHASRAIVILSTCLAFPTISEADATTFRVRDDGKSKTTFVSDA
                     PLETMTGNTSKVTGSLSVDPSDITTTTGSFQAPVVSLRTGNELRDEHLQGDGWLDAKK
                     YPNIYFEISEVILGKKGSAELKPKKDVKVEVKGKFTAHGITKLVVAKGTVSWSDKTLR
                     IKAKFPVTLEDHEISVPSIVRLKVANEIAVSVDLLAVAD"
     gene            complement(3165..5192)
                     /locus_tag="VFG22_07440"
     CDS             complement(3165..5192)
                     /locus_tag="VFG22_07440"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HET6416114.1"
                     /translation="MPNPLIQLGFEIPFDQIEAAHVEPAIDTLLAESSAAIDSIVANE
                     EPRTYANTLGALEAATEMLERASTVVGHLESVATNEALREAYNVTHPKVSAFWSDLAM
                     NDGLYDAVRAFSKTDEAKALPPTEARFLKKTLDDFRRHGAELDSEAKARLREIEVELT
                     KLTTQFSQNVLDETNAFELLIEDEAKLAGLPESAKQAAAENAKTKGAEGWRFTLHAPS
                     MIPVLTYLDDGAIREQVWRAYNGRAVSGPRDNRPIITRILELRRAKAELLGYTDFSDL
                     VTEDRMAKAGATARAFIEDLRGRTLDAFARENRQLQAYRAELEGDSAPALEPWDLGYY
                     AEKQRQAKYDFNEEELRPYFPLDRVLGGLFSTAEALYEIEIVEREAATWDPEVKSYAI
                     RERDGTLIAAFYVDLFPRENKRGGAWMNSLISAAYRESKPRPHLGLFCANVNPPVGGK
                     PALLTHREVETLFHEFGHLLHHCFSRVSVRSLAGTNVAWDFVELPSQIMENWCWERSG
                     LDLFAAHYETGERVPEALYEKMVRARTYRAANAQMRQLGFSAMDLALHQDYRPSRDGD
                     VMAYAKEIIQTYSAAALPDDYAMLAGFTHVFAHPVGYAAGYYSYKWAEVLDADAFTRF
                     KKEGITNPKVGRAFRDTILSKGDSEEAEKLYRDFMGRGPELGPLLERAGLG"
     gene            5301..6197
                     /locus_tag="VFG22_07445"
     CDS             5301..6197
                     /locus_tag="VFG22_07445"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HET6416115.1"
                     /translation="MAVPTRYILHQGPVLGALARAAFVALRSASGQPNSAPSEIPGPV
                     ISARIPPRPADLVRDYIRHVGGDPAWYRGQVPAHLFPQWVFPLQARGIEGLQYPIQRV
                     LNGGSRLQMNAPLPLGEPYELKVQLLDIDDNGRRAVIHQRATTSTSAHPEAIVADVYG
                     IVPLGGGDKSAKRKDDRPRVPVMAKELARWKLGTHVGLEFAKLTGDFNPIHWIPPAAR
                     ANGFRNVINHGFSTMARAIEGLNRSLFSGDVSRLEAIDVKFTKPLVLPAKVGLYIEDD
                     SVFVGDAPGGPAYLVGTYSVRS"
     gene            6204..7628
                     /locus_tag="VFG22_07450"
     CDS             6204..7628
                     /locus_tag="VFG22_07450"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF006110.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HET6416116.1"
                     /translation="MTDLLLENQNARKLIKTLGLPIPVPERLARSKAPYEERPLDDKT
                     VLVGGAGGLQGVLAQILTKAGATPWVVGESDAVLEPYLGPGEAWARTPHRVDPGDAPE
                     GQRIDAIVFDGTGLESPDDLRQLYEFMHPWIRRLNRSGRVVVIGRPSATAKKPARAAT
                     RAGLEGFTRSLAKEIGANGSIANSVFVDEGAEDRLAPVLRFLLSPRAAFISCQPFHVS
                     MNAKGEEAPETHVLGGKVALVTGAARGIGEATAELLAAEGAHVVCLDRPADDGPCSQV
                     AQRIGGSTLLVDITDEDAPSRIASELKERFGGVDIVVHNAGVTRDKTLGRMKADAWDM
                     AVDINLGAVIRITDRLLEDAVLRPGGRIICLSSVSGIAGNRGQTNYSASKAGIVGFVE
                     ALANKVGKKGITVNAIAPGFIETRLTSAMPVAIREAARRLSALGQGGKPEDVGQAITF
                     LATPGAAGLTGQTVRVCGGGLIGA"
     gene            7631..8941
                     /locus_tag="VFG22_07455"
     CDS             7631..8941
                     /locus_tag="VFG22_07455"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HET6416117.1"
                     /translation="MAKKKNGWGKKQRAVIVAGVRTPFVKAFGKFLKLDSIDLGDIAV
                     RSLLERTDLPHAEIDSIVWGGVILPSHAPNIAREIALDLRLPPGVEGMTCTRACASGL
                     QAVTLAAAAIERGEADVVIAGGSDSTSNAPLNLPQKVVHALAPLAFGKASTPADYLGV
                     LSQMMPITEILPKMPKIAERTTGEVMGESAESMARRNEISREAQDRFAERSHHRAAAA
                     IASGRFDDEVTAVETPSGWVYADNLVRGDTTYEKLAKLRPVFAKDGTVTAGNASPLTD
                     GAAAVLLMSEEKARALGYRPLAAFRSWSYVGVDPADQLLIGPALAMPLALDRAGMELE
                     DISVVDMHEAFAAQVLSVTKALASQGFAEVRLGRSKAVGEIDDDKFNLYGGSLSIGHP
                     FGATGARMVTTMANELANTGKETALLGICAAGGLGAAAVLEAVR"
     gene            8944..9822
                     /locus_tag="VFG22_07460"
     CDS             8944..9822
                     /locus_tag="VFG22_07460"
                     /inference="COORDINATES: protein motif:HMM:NF024841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HET6416118.1"
                     /translation="MRVGAAVAVTLLLACASCKKTEPRGPGRIVALETDVAGLSGLTV
                     DEHGAFWAPGEDGDAIIRIDPETFGVTRYLVSGGPPATDLEAMAWVDGVRFILGTETQ
                     EKGRLQDLILDGRLDGDRFAVAPIGQIDYARWQLTAPDNHGIEGICHVDGILILATEL
                     VELQRGRRWAPLGMFDPRTQSWTAHRVGLGSKTGKLAAMSCRLVDGAIVALAVERHFG
                     VSRLIRFQIPRGPEGHWIEPRVVLDLSKSIEPLPNFEGLAWMEDGSAVLLSDNKYGRG
                     AREPSRLYFIPASSIQ"
BASE COUNT         1835 a         3044 c         3141 g         1870 t
ORIGIN      
        1 cataggcgct ctcggcaggg caaacgatgt cgtagcggcc ctgcacgatg atcgccggta
       61 tgtggcggat gatgccgacc tgcctcagca gtcgatcgtc ttcctctaag aagccggcat
      121 tgatgaagta gtggcattcg attcgggcga acgcttccgc aaatgtgacg tctccggtac
      181 gttcgatcag cacggggtct ggaatcagtc ggctcgtact gccctcccac atgctccatg
      241 cgcgggcggc gcggatgcgc tcttcccgat ccgagctcgt gagtcggcgg tggtacgcgc
      301 tcagcatgtc tccacgctct tcttccggga tcggctccag gtacttctgc caggcttccg
      361 ggaagattcg gctcgcgcct tcctggtagt accaatcgat ctcgcggcgc ctgagcagga
      421 agatgccgcg aaggatcaat tcagtgacgc gttcgggatg cgcttgcgcg taggccaggg
      481 aaagcgtgct gccccaggag cccccgaaca gttgccagcg ttcgatgccg agatgcacac
      541 gaatgcgctc gatgtcgtcg actaggtgcc aggtcgtgtt gttttgcaac tccgcgtggg
      601 gcgtgcttcg gccgcaacca cgctgatcga agaggatgat cctgtacttc gacggatccc
      661 agaaccggcg gtgcaccggg ctggagccgc cgccgggccc tccgtgtacg aaaatcgcag
      721 gctttccttc cggattgccg cactgctcgt agtaaatcga atggatcgag tcgacgcgga
      781 ggtgtccgga atcgtagggc tcgatctctg gaaatagctc tgccattggg agttggcatc
      841 tcagcacgtt cgcggactgg cgcaactgac gcgagtgact atcttgccgc ccgggcatgg
      901 gccagaacga atatcaacgg cgacgaacgt tcgcgatcat ctcgcatcca gacgccggca
      961 agacgacttt gaccgaaaag cttctgcttt acggaggcgc gattcatgcg gcgggcgccg
     1021 tcaagtctcg caaggccaag cgctctgccg tgagcgactg gatggcactc gagaagcaac
     1081 gcggtatttc ggtcaccaca tcggtcctcc agttcgagta cgacgaggtc cgctggaatc
     1141 tgctcgatac gccgggacac aacgacttca gcgaagacac ctacagaacg ctcatggcgg
     1201 cggactgcgc aatcatgatc ctcgacgcgg cgcgaggcgt ggagccgcaa acacgcaagc
     1261 tcttccacgt ttgccgaatg cggaacaccc cggtcgtcac cttcatcaac aagatggacc
     1321 ggcctgctcg ggatgcgttc gccctgatga gcgaggtcga agaagttctc ggcgtgagca
     1381 cggtgccgta cacatggccc atcggaagcg gtgatttgtt tcagggcgtc tacgaccgca
     1441 tcaacaagct cgtgcttcgg ttcgagcgat cgtccgaggg ccaggccaaa cgcgcggaga
     1501 tgaaagtcac cggactcgat gaccccgagc tcgacacctt gctgggtgcg gaggcggcgc
     1561 agacccttcg cgacgaggtc gagcttctcg acggtgcggg cgagcaatgg gacgaagaca
     1621 ggtttcgcgc cggcgagatc acgccggtct tcttcggcag cgcgctcacg aatttcggtg
     1681 tcgagccgtt tcttcgggcg ttcaaggggc tatgtccgcc acccgggaaa cgtgaggcat
     1741 cgggcgacgt cgtcgtgagc cccgacgacg atcgcttcag tgcgttcgta ttcaaagtcc
     1801 aggccaacat ggatccgtcg cacagagacc gaatcgcgtt tgcacgcatt tgctcaggca
     1861 agttcgaagg cgggatgcgt gttcaccact accgcctcgg aaaagagatt cggctcaacc
     1921 gcgcggagca gttctttgcg caagagcgtg aaagcgtctt ggaagcctac gccggcgata
     1981 tcgtaggcct ctacgatccg gggctctttc gcattggcga cacgctttct acgaacggac
     2041 ccatgagctt cgatgcagtt ccacggttct ctccagagca tttcggacga ttgcagcttc
     2101 tcgacccgct caggcgcaag caactcctca aaggaattca ggagctttcg gaagagggca
     2161 ccgtgcagct ctttctcgag ccgggacgtg agaaggatcc agtctgcggt gtcgtcggcc
     2221 gcttgcagtt cgacgtgctg atgtttcgac tcgagcacga gtatggtgcg aaggtgacgt
     2281 tcgacttgct tccgtatcag catgcacgtt gggtcgaggg agccgcggat tgcaccgagc
     2341 tgacggccaa gcgggttccg atggcagttc tcgatcggga cgatcgcccc gtcgccttgt
     2401 ttcgcgacga atgggagctt ggccgcgccg agcgggataa cgagaattgg accttccacg
     2461 agaccgcccc gatattcgta gcggcctgaa tacggggatg aagcgcccgt ccaaaaccgg
     2521 catactggaa atcaggggga acgagcgaat gatgaagcac gcgagcagag cgattgtgat
     2581 cttgtccaca tgtttggcct ttccgacgat ctccgaggcc gatgcaacga cctttcgagt
     2641 tcgtgacgat ggcaagagca agaccacgtt cgtttcggac gcgcccctcg agacgatgac
     2701 gggcaatacc tccaaagtga ccggcagcct ctccgtggac ccttctgaca tcactacgac
     2761 gacaggctcg ttccaggcac cggtcgtgtc acttcgcacc ggaaacgagc tccgcgacga
     2821 gcaccttcaa ggtgacggct ggctcgacgc gaagaagtat ccgaacatct acttcgagat
     2881 cagcgaggtg atcctcggaa agaagggctc agccgagctc aagccgaaga aggacgtcaa
     2941 ggtcgaggtc aaaggcaaat tcacagccca cgggatcacc aagctcgtcg tcgcgaaagg
     3001 caccgtgagc tggtcggaca agacacttcg gatcaaggca aagttcccgg tcactctcga
     3061 agatcacgag atttcggtgc cgtcgatcgt gcgactcaag gtcgccaacg agatcgccgt
     3121 atcggtcgac ctccttgcgg tcgcggacta gttcgcggca gttttcatcc cagtccagcg
     3181 cgttcgagga gtgggccgag ctcaggaccc ctccccatga aatcccggta gagcttctcc
     3241 gcttcttcgc tgtcgccctt cgagaggatc gtatcgcgga acgctcgccc gaccttgggg
     3301 ttcgtgattc cctctttctt gaagcgcgta aatgcgtcgg cgtcgagcac ctcggcccac
     3361 ttgtagctgt agtagccagc cgcgtagccg accgggtgag caaagacatg ggtgaaccct
     3421 gcgagcatcg catagtcgtc ggggagcgct gccgccgagt acgtttgaat gatctctttg
     3481 gcgtacgcca tcacgtcccc gtcgcggctg gggcgatagt cctggtgaag tgccaagtcc
     3541 attgcggaga agccgagctg acgcatctgc gcgttggccg ctcggtaggt tcgcgcgcgg
     3601 accatctttt cgtacagcgc ctcgggaaca cgctcgcccg tctcgtagtg cgccgcgaaa
     3661 agatcgagcc cgctccgctc ccagcaccag ttctccatga tctgtgaggg aagctcgacg
     3721 aagtcccagg ccacgttggt tccggcgagg cttcgaacgc tcacgcggct gaagcagtgg
     3781 tgcagaaggt ggccgaactc gtgaaagagc gtttcgacct cgcgatgcgt gaggagcgcc
     3841 ggctttccac ccaccggcgg gttcacgttc gcgcaaaaga ggcctaggtg cggccggggc
     3901 ttcgattccc tgtatgccgc gctgatcaac gagttcatcc aggcgccgcc acgcttgttc
     3961 tcgcgcggga atagatcgac gtagaatgct gcgatcagag tgccatcgcg ttcacgaatc
     4021 gcatacgact tgacctccgg gtcccaagtc gcggcctcac gctccacgat ctcgatctcg
     4081 tagagcgcct ctgcggtaga gaagagtcct ccgagcaccc ggtcgagcgg gaagtagggc
     4141 cggagctcct cttcattgaa gtcgtacttc gcctgacgct gcttctcggc gtagtagccc
     4201 agatcccagg gctcgagtgc cggcgccgag tcgccttcga gctcggcccg gtaggcctgc
     4261 aactgccgat tctcacgtgc gaatgcgtcc aacgttcgcc cgcgcaggtc ctcgatgaag
     4321 gctcgtgcgg tagcgcccgc cttggccatg cggtcctcgg tcaccaggtc cgagaagtcc
     4381 gtatatccga ggagctccgc cttggcgcgt cgaagctcga ggattcgcgt gatgatcggt
     4441 cggttgtcgc gggggccgga gacggctctg ccgttgtacg cgcgccagac ttgctcgcga
     4501 atcgcgccgt cgtccaagta agtcaggacc gggatcatgc tcggggcgtg aagcgtgaag
     4561 cgccagccct cggcgccctt tgtcttggcg ttttctgcag cggcctgctt tgcgctctcc
     4621 ggcaagcccg caagcttcgc ttcgtcctcg atgagcaact cgaacgcatt ggtttcatcg
     4681 agcacgttct gggagaactg ggtcgtgagc ttggtcagct cgacctcgat ctctcgaagc
     4741 ctagctttgg cttcggagtc gagctctgct ccgtgtcgcc ggaagtcgtc caaggtcttc
     4801 ttcaggaacc gagcttcggt cggtggaaga gccttggctt cgtcggtctt ggagaaggcg
     4861 cgaacggcgt cgtagaggcc gtcgttcatc gcgagatcgg accagaaggc gctcaccttc
     4921 gggtgcgtga cgttgtaggc ctcgcgcagt gcctcgttcg tcgcaacgct ttccagatga
     4981 ccgaccaccg tgctcgctcg ctcgagcatt tcggtggcag cctcgagtgc gccaagggtg
     5041 ttcgcgtaag tacgcggctc ctcgttggcc acgatcgagt cgatcgctgc ggaggactcc
     5101 gctagcagcg tgtcgatcgc cggctcgacg tgagcggcct cgatttggtc gaatgggatc
     5161 tcgaatccga gctggatcaa cgggtttggc atcgtgcaag cctagcagcg cgaccgggtt
     5221 tgcgtggttt tctaccaaat ggtagagact cgcctcgggc gacagggact ccaccgcacc
     5281 gacgagccgg gagaatcgat atggcggtac ctacgcgtta catcctccat caggggccgg
     5341 tgctcggcgc tcttgcccgt gcggctttcg tcgcgctgag gagcgcgtcc gggcagccga
     5401 acagcgcgcc gagcgaaatc ccgggccctg tgatttcggc gcgcattcca ccacgacccg
     5461 cagacctcgt ccgggactac attcgacacg tcggaggtga tccagcctgg taccgcggcc
     5521 aagtgcctgc gcacctgttc cctcagtggg tcttcccgct gcaggctcga ggcatcgaag
     5581 gtcttcagta tccgatccag cgcgttctaa atgggggcag ccgattgcag atgaacgccc
     5641 cgctccctct cggcgaacca tacgagctca aggtgcagct gctcgacatc gacgacaacg
     5701 gacggcgtgc cgtgatccat cagcgcgcca ccacctcgac gtcggctcat cccgaggcga
     5761 tcgtcgccga cgtgtatggc atcgtgccgc tcggcggtgg agacaagtcc gccaagcgca
     5821 aagacgaccg cccccgcgtt ccggtgatgg ccaaggagct cgcgcgttgg aagctaggca
     5881 cgcacgtcgg tctcgagttc gcgaagctca caggcgattt caatccgatt cattggatcc
     5941 cgcctgcggc ccgggccaac ggttttcgca acgtcatcaa ccatgggttc tcgacgatgg
     6001 cgcgtgcgat cgaaggactc aatcgttcgc tcttttcggg cgacgtgagt cggctggaag
     6061 cgattgacgt gaagtttacg aagcctctgg tgcttcccgc caaggtcgga ctctacatcg
     6121 aagacgacag cgtgtttgtg ggtgacgctc ctggcgggcc cgcgtaccta gttggaacct
     6181 attcagtgag gagctgagct caaatgactg atcttctgct cgagaatcaa aacgcccgaa
     6241 aactgatcaa gacgctcggc cttccgatcc cggtgccaga gcggctcgct cgatccaaag
     6301 ccccgtacga agagaggccg ctcgacgaca agaccgtgct ggtgggaggt gctggcggct
     6361 tacaaggcgt cctcgcgcaa attctgacca aggcgggcgc caccccgtgg gtcgtgggcg
     6421 agtccgacgc cgttctcgag ccttacttgg gaccaggcga ggcctgggcg agaacgccac
     6481 accgcgtgga ccccggcgat gcgcccgagg gccaacggat cgacgccatc gtcttcgacg
     6541 gcacgggtct ggagtctccc gacgacctgc gtcagctcta tgagttcatg catccttgga
     6601 ttcgccgcct gaatcgttcg ggacgcgtcg tcgtcatcgg ccgcccgtcg gcgaccgcga
     6661 agaagccggc gcgcgcggca acccgcgcag gcctcgaagg cttcacacgc agcctcgcaa
     6721 aggagatcgg cgccaatggg tcgatcgcga atagcgtgtt cgtcgacgaa ggcgcagagg
     6781 atcgcctcgc tccggtgctt cgctttcttc tttcgcctcg ggccgctttc atcagttgcc
     6841 agccgtttca cgtcagcatg aatgcgaaag gcgaagaagc gccggagacc cacgtgctcg
     6901 gaggaaaagt ggcgctggtg actggcgcag cgcggggcat cggtgaagcg accgccgagt
     6961 tgctcgccgc cgaaggcgcg cacgtggtct gtttggatcg ccccgcggac gatggtccct
     7021 gcagccaagt cgcacagcgg atcgggggct cgacactgct ggtagacatc acagacgagg
     7081 acgcgcccag ccgcatcgcc agcgagctca aggagcgttt cgggggagtc gacatcgtcg
     7141 tccacaatgc cggcgtgacg cgcgacaaga ccctgggccg catgaaagcc gacgcctggg
     7201 acatggccgt cgacatcaat ctcggcgcgg tgatccgaat caccgaccgg ctgctcgagg
     7261 atgcggtgct gcgtccgggc gggcgcatca tctgcctctc ctctgtttct ggaatcgccg
     7321 gaaatcgggg acaaaccaat tattcggcaa gcaaggcggg catcgtcggt tttgtcgagg
     7381 cgctcgcgaa caaggtcggc aagaagggga ttacggtcaa cgcgatcgct cctgggttca
     7441 tcgagacccg gcttaccagc gcgatgcccg tcgccattcg tgaagccgcg cgccggctga
     7501 gcgcgctagg gcagggcggc aagccagaag acgtcggcca agcgatcact ttccttgcaa
     7561 cccccggagc ggcaggcctg acgggtcaga ccgttcgagt ctgcggtggc ggactcatag
     7621 gagcatgaac atggcgaaga agaagaatgg ttggggcaag aagcagcgcg cggtcatcgt
     7681 ggccggtgtg cgcaccccgt ttgtcaaagc cttcgggaag ttcctcaagc tcgacagcat
     7741 cgacctcggc gacatcgcgg tgcgctcgct cctcgaacgc acagaccttc cccatgccga
     7801 gatcgacagc atcgtctggg gaggtgtcat cctgccctcg cacgcgccga acatcgcccg
     7861 cgagatcgcg ctggacctcc ggcttccgcc tggcgtcgag ggcatgacct gcacccgagc
     7921 ttgcgcatcc ggccttcaag cggtgacgct cgctgccgcg gccatcgaac gcggcgaggc
     7981 ggacgtggtc atcgccggtg gatcggattc cacgagcaac gcaccactga acctgccgca
     8041 gaaggtagtc cacgcgttgg cccctctcgc gtttgggaag gcgtccacgc ccgctgacta
     8101 cctcggtgtg ctctcccaga tgatgcccat caccgagatc ctgccgaaga tgcccaagat
     8161 cgccgagcga acgaccggcg aggtcatggg cgagtccgct gagtcgatgg cgcgtcggaa
     8221 cgagatctcc cgagaggcac aggatcgatt tgccgagcga tcgcaccatc gcgctgccgc
     8281 ggcgatcgca tcaggccgtt tcgatgacga agttacagcg gtcgaaaccc cgagcggatg
     8341 ggtgtacgcg gacaacctcg tccgtggcga caccacctac gaaaagctcg caaagcttcg
     8401 ccctgtcttc gcaaaggacg gcacggtcac cgcgggcaac gcgtcgcctc tcaccgatgg
     8461 cgctgcggcg gttctgctca tgagcgagga gaaggcgcgc gcactcggct acaggccact
     8521 cgcagcgttc cgcagttggt cgtacgtcgg tgtcgatccc gccgatcagt tgctgatcgg
     8581 gccggcgctc gcgatgccgc tggcgctcga tcgtgcgggc atggaactcg aggacatcag
     8641 tgtcgtcgac atgcacgagg cattcgctgc gcaggttctt tccgtgacca aggcgctggc
     8701 gagccaggga ttcgccgagg tcaggctcgg taggagcaag gcggttggcg agatcgatga
     8761 cgacaagttc aacctctacg ggggctcact ttcgatcgga catccctttg gggccactgg
     8821 agcccgaatg gtgaccacca tggcaaacga gctcgcgaac accggaaaag agaccgctct
     8881 tctcggtatc tgtgcggcgg gcggccttgg cgcggctgcc gtgctcgaag cggttcgctg
     8941 agcgtgaggg tcggggcggc agttgccgtg acgcttctgc tcgcctgcgc cagctgtaag
     9001 aaaacggaac cgcgcggccc gggtcgtatc gtcgcactcg agaccgatgt ggccggccta
     9061 tcgggtctca ccgtagacga gcatggagcg ttctgggctc ccggcgaaga tggcgatgcg
     9121 atcattcgga tcgatcctga gaccttcggt gtcactcgct atctcgtctc gggtggcccg
     9181 ccggccaccg acttggaggc tatggcctgg gtcgacggag tgcggttcat cctcggcacc
     9241 gaaacacaag agaagggacg tctccaagac ctgattctcg acggccggct cgacggagac
     9301 cggttcgcgg tcgcgccgat cggccagatc gactacgcgc gctggcaact cacggcccca
     9361 gataaccatg gcatcgaggg gatttgccat gtagatggca tcttgatttt ggccaccgag
     9421 ctcgtggaat tgcaacgcgg acgtcgatgg gcaccactcg gcatgttcga ccccaggaca
     9481 caatcttgga ctgcgcaccg cgtaggcctc ggcagcaaaa ccggcaagct cgcggcgatg
     9541 agctgccgac tcgtcgacgg cgcgatcgtt gcgcttgcgg tcgagcggca cttcggggtc
     9601 tcgcgtctca ttcgctttca gattcctcgg ggccctgagg ggcattggat cgagccgaga
     9661 gtcgtgctcg acctgtcgaa gtcgatcgag cctcttccga acttcgaagg tttggcctgg
     9721 atggaggatg gctcggccgt tcttctcagc gacaacaagt atgggcgcgg cgctcgcgaa
     9781 ccaagccggc tctacttcat tccggcgagc tcgattcaat gaggcagagc gctttctgct
     9841 tgatgctggc ggtcggcatt gccgggcccg ctcaggccgc gcccctcgag
//
LOCUS       DASRZF010000165        30385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3203268, whole genome shotgun sequence.
ACCESSION   DASRZF010000165 DASRZF010000000
VERSION     DASRZF010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 30385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30385
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3203268"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="VFG22_07465"
     CDS             <1..512
                     /locus_tag="VFG22_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HET6416119.1"
                     /translation="GLHYLGGLVTLAPELTALYSPFVNSYKRYVLGVWAPLNASWGIE
                     NRTCGSRVILGPDDAATRIEFRQTAADINPYIAMAACLGSGLYGIEHEIDPPAMTSGD
                     ATSDSGPSLGLPLTLEAAVERLRESQAARAALGDDFVDHFTRTRDWECREYRKAVSDW
                     ELRRYFEAV"
     gene            522..2228
                     /locus_tag="VFG22_07470"
     CDS             522..2228
                     /locus_tag="VFG22_07470"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HET6416120.1"
                     /translation="MFKRFLSERPLRLPHLRPYVPRPSDPSIFWFNSERDDVVSDVVK
                     RICDDHETDRERLEMALNDAAFHEIRRLEKQRDEEARDRLGYWRSMLRRIGKMDDVEQ
                     RRVLHTIVTNMTRDVAGNFDPRVYRFARRAIPRLIGGVMEPRSLGKELVGSASDSLDR
                     VLKVQGDLEHLRYLQGEGSLVFVPTHSSNLDSIVLSQALETSGLPPVVYGAGKNLFTN
                     PIISFFMHNLGAYRVDRRIRVSLYKDVLKLYSQVMIERGYHSLFFPGGTRSRSGMIER
                     HLKLGLLGSAVEAFTTNRVRRVDRPVWFVPTTINYALVLEAETLVKDWLTEEGQARYI
                     IEDDEFSRIDRWFAFFRKMVGMRGACIIRFGEPIDPFGNRVDGRGQSLTPTGRTIDAG
                     GYVERRGTPVADPARDAAYTRELGEVLAKRYEQDTVLMSTQVVAHVLYRDLVQTTPGM
                     DLFARQRLRGEVGLDRESLCRELGEARDRLVALEAEGRVRTSDVVKEDSPDEMLERAL
                     SFWSGYHDSTAATTEGNRIVIGDPSLLLYYQNRLVPFAEAIADPGQLRAACEIENLGA
                     SR"
     gene            2225..3160
                     /locus_tag="VFG22_07475"
     CDS             2225..3160
                     /locus_tag="VFG22_07475"
                     /inference="COORDINATES: protein motif:HMM:NF019122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET6416121.1"
                     /translation="MSLRIGVVGAGGFGRAIALASARHGHDVWLWSRRERELPDPIRV
                     STDLMALRECELLFLAVPSRHAEEVSEQLCEVVDGRHLMVHVSRGLIGAELKTITQVI
                     REKTVCRRLGALAGPIVAEALAEGRPSGAILGTRFPELTELVREALGGSELRMYETRD
                     VIGVELASATVGFLTVVLAYAQEIGVDPGTLAVLATRGMVEVSRIGQTLGADESTFMG
                     LAGLGDLIAAVAGDQRPEQLLGRALAQGADLSNAVGKAGAYIEGIEVARHLMEHARRL
                     GLETPISSTFVALSEGRISPDDAMHALMERRVGKE"
     gene            3157..4332
                     /locus_tag="VFG22_07480"
     CDS             3157..4332
                     /locus_tag="VFG22_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HET6416122.1"
                     /translation="MTDSKDLVIWSFPTRVLLGNGAAARCAEEAIRLGASRVLLVSDK
                     GVERAGLLDSIRSALDAAGLQHQSALEISSNPLEAEVLGATREFDQLKADLILAVGGG
                     SVLDVAKLVRLAATHPGPLAQYDDAIGGGDKINGPLPPMIAIPTTAGTGSEVGRSGVV
                     TIQSTNRKTVIFSPLLLPDVAILDPEMSRTMPPSMTAATGMDALTHCIEAYASIGDHP
                     MADAIALGGVRLCQQSLKAATDDGDDLKARADMLKAAMMGAVAFQKGLGACHSLAHPL
                     SAEFGMHHGLANAICLPAVLDFNRAAVPERIAEVARELGVRGNDESTLSFECAGAIRA
                     LRKSIGLPSNLRSQGVEESDLPRLAALAFADSCHQLNPRRCTEDDLLALYKAALDDD"
     gene            4325..4801
                     /locus_tag="VFG22_07485"
     CDS             4325..4801
                     /locus_tag="VFG22_07485"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HET6416123.1"
                     /translation="MTKRKKGQLESIGKLLKGVYPAPEQLEAAKVFAWWSRSVPPRIL
                     EHARPVRLSRGVLIVHVSSSVWANELHYLSDDLLTRIREAAPTTGIERIRFQVGPLPD
                     IPKHAQPAPAPPQPIRLASLPEELGRALSRVEDDALRTAIADAASTSLARIREPKD"
     gene            complement(4830..5153)
                     /locus_tag="VFG22_07490"
     CDS             complement(4830..5153)
                     /locus_tag="VFG22_07490"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET6416124.1"
                     /translation="MATIDVISANLPILRELRSRLGQLELADRVGVSRRTIARLENAE
                     VADPGVDLMKRIAGELGVTLHVLTESKLGATTVPLPDDVSSQLASADGAETLEAMIRA
                     ARQTR"
     gene            complement(5194..5994)
                     /gene="trpA"
                     /locus_tag="VFG22_07495"
     CDS             complement(5194..5994)
                     /gene="trpA"
                     /locus_tag="VFG22_07495"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HET6416125.1"
                     /translation="MGRIADAFENARNEKRAALVIYLCAGDPDLGTTVDLVVAAAEAG
                     ADVIELGVPFSDPTADGPAIQRAAERSLRGGTNVKKVLDTVRVIRGRTDVPILLFGYY
                     NPVLAYGERKLVDDAAQSGADGFLIVDLPPEESAALREPAVAKGLDFVPLIAPTSDPA
                     RIRQAGDAATSFIYYVSMTGVTGSQAADLVNASKRAGVLQRELGRPLALGFGVKTKED
                     VRMVAPNVDGVVVGSAVVQAIEEASSSDEAVAAVRTLVTDLARGLTRQ"
     gene            complement(5996..7210)
                     /gene="trpB"
                     /locus_tag="VFG22_07500"
     CDS             complement(5996..7210)
                     /gene="trpB"
                     /locus_tag="VFG22_07500"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HET6416126.1"
                     /translation="MRYVPRLMQPPEPTAGRYGPYGGRYVAETLMPALDELTQAYQES
                     QRDPEFQAELDSLLHQYAGRETPTYFASRLSEKVGARIWLKREDLCHTGAHKVNNTIG
                     QVLLAKRMGKTRIIAETGAGQHGVATATACALMGLPCEVYMGAEDVRRQAPNVRRMEL
                     LGAQVHSVTSGSSTLKDAMNEALRDWVTNVRTTHYCVGSVAGPHPYPMIVRDYQSVIG
                     QEARAQMLKQAGRLPDAVVACVGGGSNAMGIFAGFIDDPSVRLIGVEAAGEGIETGHH
                     AAPLCAGGVGVLHGAKSYVLQTADGQIVEAHSISAGLDYPGVGPEHAWLKDTGRATYT
                     AVTDDAALDAFQQLCRIEGIIPALETSHAFAYLPTLAKELGPDADILVCLSGRGDKDL
                     ETVLEKLKERAN"
     gene            7226..8026
                     /locus_tag="VFG22_07505"
     CDS             7226..8026
                     /locus_tag="VFG22_07505"
                     /inference="COORDINATES: protein motif:HMM:NF015915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET6416127.1"
                     /translation="MHMTQPSNTTIPPLNREAYERMLQRTSFLSEWFRPSIEGLENIP
                     TKEGALLVTNHGHFGIDLPVLLRLILEGTGRAVRSLGDRIVFATPFFRDIAHTMGAIE
                     GEPETTVHLLEDDQLVLVYPGGAREALGDPKDAYRLQWEQSRGFIRTALRAQRPIVPV
                     AGLGNEELYVQVVPQDRVRKSGIGRLITQLLGDKYVMPIYMGLGALPFPTELHYIIGK
                     PIHLPYGPEAADDPEVVAGLHHEVTETTQRLIDGGLDARKQLGLADIG"
     gene            complement(8037..8630)
                     /locus_tag="VFG22_07510"
     CDS             complement(8037..8630)
                     /locus_tag="VFG22_07510"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6416128.1"
                     /translation="MHLPDTSRIRRADSSDLDAVLDILADAFEMDPVMRWVTRNPNYP
                     RYVFSLTVPFCLGHGLTHLAEDGSGAASWLPPGVALESPVSPSVVWDGLTRYGPRSLL
                     RAFATLIQTQKRHPREDFYYLFAIGTRQAHRARGVGGALMRAGLERCDEDRMPAYLES
                     SNVNNLPFYRAHGFEIVEEIRLAMGGPPMWLMWREAR"
     gene            8664..9215
                     /locus_tag="VFG22_07515"
     CDS             8664..9215
                     /locus_tag="VFG22_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416129.1"
                     /translation="MKYKLSRRTFTKLGVLSGASALVLPGCSDESVITEGSAKLTADE
                     RELLERFAEVYLPTEGTSLKPRSEVPVVDNIEHAFSLMDAPTLEQVRIGLKLFNLGSI
                     LIGLHFARFTHLSAEHRLAYVRKWEEGVEVQRGISTLLKKLVCYGYWKDIEAGRAIGY
                     QGPVSEASGIVSLGNAPMPATPS"
     gene            9227..10780
                     /locus_tag="VFG22_07520"
     CDS             9227..10780
                     /locus_tag="VFG22_07520"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HET6416130.1"
                     /translation="MDESNPAIQERPVLKVTAPSAIPDAGLTLVADVVVIGSGAAGAV
                     TAYELARSGAKVVVLEAGPHIPSSDFKEHAPTALETLYEDHGNQVNTTGDLTILQGRC
                     VGGSTVVNAAACFRVPDQVLEHWTTEHGLDNLSPDALAPYFEKVERNLSIRQNAAHEV
                     NYNGRLLIEGAQKLGIPASPISRNVKDCALSGHCISGCKTDRKQSMLVTYLPWASELG
                     VTILSGTRAETFQTSGRRVTSVNAVATDENGNQKPVRVEAGLVLVAAGAVQTPLLFQK
                     NALANSSGQIGLNFACHPSLAVFGEHEGDVFAWVGATLTAQAGDIENPLKGGYLLEAG
                     MVGPVVTSSWIDGGLGRDFASFMENSKKYQAAVTLIHDHNVGRVYLEGDRKRIEYDLD
                     DRDFESMREALRGTARIYFAAGARRVYLPTTRRTTIESAEQIDSVIDRLANGKHMYRM
                     NSYHPQGTMRMGADPAKSVVGPDGRCHDLDNVYVPDASLFPSSLLVNPQVTVYTMASY
                     ISDQILARA"
     gene            complement(10781..11179)
                     /locus_tag="VFG22_07525"
     CDS             complement(10781..11179)
                     /locus_tag="VFG22_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416131.1"
                     /translation="MRLTILASLFTAAAMWIALGGNPAQSQEQAKPASCQTCGQGAAG
                     TSCPHCAKGQACPHCDQGKACPHCKSGPHHGWHAAHKHEYKCVHQSERASKKAAEEMS
                     AQFSALGEDGWRLAKADAGFWCFSRIRGAQ"
     gene            complement(11296..11910)
                     /locus_tag="VFG22_07530"
     CDS             complement(11296..11910)
                     /locus_tag="VFG22_07530"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HET6416132.1"
                     /translation="MVEVKVCGLTTVDDTLRCIEAGADAIGLNFWPRSPRFVDLAVAR
                     RIVAAARDDARMVGVFVDLDLAQIKDILAETGIEWAQLHGSEPPDLVAALLPRAYKAI
                     GVKDGSAIELARSYPGEHLLLDASVPGLPGGTGRTFDWKIAAEVAKERKLILAGGLTP
                     SNVADAIRAVNPQQVDVASGVESAPGRKDPSLVRAFIRAAKNSP"
     gene            12057..13934
                     /locus_tag="VFG22_07535"
     CDS             12057..13934
                     /locus_tag="VFG22_07535"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HET6416133.1"
                     /translation="METSAAARVRPSPRETLDSVVIRFAGDSGDGMQLTGSQFTTATA
                     DAGNDLSTFPDFPAEIRAPAGTTYGVSGYQIHFASHDVQTPGDAPDVLVAMNPAALMV
                     NSEALKPGGLVVINTGAFTANNLKKAGYENNPLEDGSLDRFRVLALDVTKMTLDAVQE
                     VGLGSKDAGRCKNMWTLGLMYWLFGRERDQTVSWLKKKFAKTPKLAEANIAALNAGHI
                     YGENAELPHGINAYDVPAAKLSPGEYRNISGNEATAWGLVTGARLAGLELMYGSYPIT
                     PASNLLHNLARLKHFGVTTFQAEDEIAAVSAAVGASFGGSIGVTGTSGPGVALKSEAI
                     GLAIAAELPLVIVDVQRGGPSTGLPTKTEQSDLFQAVVGRNGDAPLAVLAAATPGDCY
                     YMAIEAVRMAVKYMTPVILLTDGFVANSAEPWLLPDLDSLRAFPVRFHKDPEGFHPFL
                     RDAQTLARVWAIPGTPGLEHRIGGLEKHRDTGHISYDPQNHHRMTKIRHQKIAGIARD
                     IEQAKVDQGESSGDLLVVGWGSTYGSISQAVKLLRERGHQVSHLHLRNIWPLPEGLGE
                     LLGSFNSVLVPELNFGQLVKLLRAEFLVDAESLTRVTGQPFRIGDLIDAVEERLQRSS
                     R"
     gene            13931..14944
                     /locus_tag="VFG22_07540"
     CDS             13931..14944
                     /locus_tag="VFG22_07540"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HET6416134.1"
                     /translation="MSPNPPKKEYDRKDFTSDQEVRWCPGCGDYAILAAVQRTLPSLG
                     STPENTVFISGIGCSSRFPYYMNTYGFHTIHGRAPAIATGLKLANPKLDVWVITGDGD
                     GLSIGGNHLLHVLRRNIDLQILLFNNQIYGLTKGQYSPTSQVGKVTPSSPLGSIDQPV
                     VPARFALGAGATFVARAFDTSKKLPEALTAAREHRGASFVEILQNCPVFNDGVFELIT
                     DRKSGPAYQLWLEHGKPLKFGANGEKGLRLNPRTLNLEVVTVGEDGVTEDDIIIHDET
                     NASLAWMLTDLPEIVALGVLYRHEGPVYNESVAKQRELAEKRFGEPDLHALLREGHTW
                     TVT"
     gene            complement(14900..15637)
                     /locus_tag="VFG22_07545"
     CDS             complement(14900..15637)
                     /locus_tag="VFG22_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HET6416135.1"
                     /translation="MDDDDRDASGEGILHPEGMRAARDLAAGIVHEVNNILGVIIGNA
                     HLAKKNLGDVEALQKYVGEVRDAAEEGRELMRNLSILASSQSPRARALSLNDLLSNVV
                     SGLGSAVELDVSVDDPVVVLDLWLAQDALSSLLRFMVATKTVTSIRIATRVLGSAAAL
                     TIEDDGASPSSEELRTLFTPFVKIDRRPKVELALTKLADLASRADGFITATVRQPHGL
                     RIVLTLPVAEARASGDGPGVSLPKKGV"
     gene            complement(15643..16017)
                     /locus_tag="VFG22_07550"
     CDS             complement(15643..16017)
                     /locus_tag="VFG22_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6416136.1"
                     /translation="MRRYRCLVVEDSKTMRNMLAESLLRIPGMEIVEAENGLTGIRAL
                     AAHRFDLVVTDVNMPVMDGLKLVRHVRSDPRHEKTPIVIITTESAPNDRDRAMALGAN
                     AYIEKPIQGPRVIRAVKKLLEI"
     gene            complement(16066..17148)
                     /gene="cheB"
                     /locus_tag="VFG22_07555"
     CDS             complement(16066..17148)
                     /gene="cheB"
                     /locus_tag="VFG22_07555"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HET6416137.1"
                     /translation="MGDLRALVVDDSSLNRRTIAAILREIPGVSSVDVANDGAEALRA
                     VESNPPDFITLDLEMPRLDGFDFLQLLMDRNPVPVVVVSGLSAKENIFRALELGAIDF
                     LAKPEGEDAPLESLRRQLVEKVRVIQQLTPLSVRRTAVGGLALDEESGAGTSANRFRE
                     PSVLKHVPSNVICIGASTGGPKALLTLFRHLHEEIDAAFVIAQHMPPRFTTTFASRLD
                     RTGVIHVSEAKQYERLARGHAYVCPGGRCIEIVPSDRGPALRVVAPDPRAHYVPSVNQ
                     LFHSAARVLGDKAIAVVLTGMGDDGAAGVRDVADRGGAILIEAPETAVVAGMPLAARR
                     SGVRHHSLGIWALGDMIAELTRPTKG"
     gene            complement(17149..18030)
                     /locus_tag="VFG22_07560"
     CDS             complement(17149..18030)
                     /locus_tag="VFG22_07560"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HET6416138.1"
                     /translation="MTLLHDAGPSLNLNEFRLLRDFVNKFCGIYFADDSAYIVERRLR
                     ERLRDLGIDDFAEYYRYLQYHPKAQAELEAAAEVLTTNETYFFREAYQLRAFTDEILP
                     VVRSRALARGSKRLDVWSAGCSSGEEVYTIAMLVDATGLFEGWDVRVFGNDISRRVLR
                     KARGATYTSASFRAMIPEYDRYFIDTAEGKQVHPRIRSMCHFGHLNLMNQRRAAIVGR
                     VDVTFCRNVLIYFDDASRRQVLDTIYERLNRGGYLLLGHSESLLQSSTAFEIAQLTTD
                     IVYRRPIPYTDLPPRRG"
     gene            complement(18032..20056)
                     /locus_tag="VFG22_07565"
     CDS             complement(18032..20056)
                     /locus_tag="VFG22_07565"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET6416139.1"
                     /translation="MTSDRLEDAFHSADPDVRRQAVYQMSLRPQGTPLKQILDALSDR
                     DWRVRREAISLAAQTVEKSALIDALLALLVETDNIGLRNAAIEVLGLCAQGQSQKFAE
                     ACAGAGEGAKKFLVEAMGKTRDPQMIDHLELLVRGSDANVAAAAVEALVCIGGARAEA
                     LLKEGLCTPHLFLRISIVEGLTRLGTRVAWEELKPAVEDTIVRRICTELLGRTGAPEA
                     LEFLIQLATDTSPQTSAAALRAIGELAEAAEDQQAELVERLGASNESFRHALHEALLH
                     GDTATRQSAAYLSVLCRDETSIEAVIQAVADDVANPETVEALRSWGPSLVEPLLMHRR
                     GEPRVWAIALELAAELSSRHLDEVPPGTRDRIRSLIERDLTDATDAVRAAAAESLRWW
                     GDARDCRALVECLNSSSHEVRAAATSALEAIAQRAPDLVEAALARADLDGPGGADIAQ
                     VLSRLASNGAVEVLKRGLHSGDPRTRRAVVQALAMADATDVAQLIGYAVADEDIDVQI
                     AAVRTLGQLRTSEANAPLRTALDSPFPPIRAEAALALGRRDASTAIPQIRVLLEDPEP
                     VVIAAALDSLAWLGDSEVEAAVERALSHSDHEVFQAALRAASTLPATDAEGFLARGLQ
                     HAAWNVRMLAIKLLLDIGTKRTRKLLTDALSIESDSMVRHAIESGLRLGD"
     gene            complement(20053..20553)
                     /locus_tag="VFG22_07570"
     CDS             complement(20053..20553)
                     /locus_tag="VFG22_07570"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET6416140.1"
                     /translation="MRAGRAFSATLAKDLIRVEVNGVMYALEVARIREIVNPLPLIAL
                     PHERPFVIGVSDYRESVVAIVDLRRLFGLPAQEENRRTKWIIIESDGKLVGVVVDAVL
                     DVFSSAEEKSREVPVLDERHRERGIASAFRHDRQLVFLLDASRLVEPAMEIPRGELPS
                     LPSEAP"
     gene            complement(20555..21046)
                     /locus_tag="VFG22_07575"
     CDS             complement(20555..21046)
                     /locus_tag="VFG22_07575"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET6416141.1"
                     /translation="MSSRAQKATLVPDPHSKGGAVAVLAVLAGDEAYGFPLSSVREIL
                     VPPPVAEVPRTAEHILGVISVRGQIITLIDLPKLLSLAVAERRSCGRVLLVDNGEELI
                     GVAVDEVIQVYRMEPAQIEYASAMGAELSEYVIGIGRMSSSGPTATDDMLILIDPVSL
                     LGG"
     gene            complement(21043..23118)
                     /locus_tag="VFG22_07580"
     CDS             complement(21043..23118)
                     /locus_tag="VFG22_07580"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HET6416142.1"
                     /translation="MSDPRDNVQQEFLAEAQELVEALSRDLLLLDEAHHRGERINPAL
                     INEIFRSVHTLKGIAGMFGYKHVGAVAHALEDLLDDLRLGRTPVAQQLLDVLFDSVER
                     FQRLLGLEDPQSPEEGPAVQAYIAAIRSIGMMPSDEEGDLLERYALDPAVLSVLTEYE
                     EHRLRACIREGLRLYRLRAAFDLTAIDDSIETLREVARDDAEIITYLPSMEGGNNETI
                     ELVVLLASRVPEQELKNHFEGRYGTIDAIPEQTHLPTLPPHLTSMPPSMATSIGPRQD
                     EDASQASLRSVANSVRVDIRKLDHLMNVVGELALVRSAIGRVVDELKRESLDRQSAMS
                     LDRIGRGFERQLAELRSGILDVRMVPLGQVFNKLARIVRQAAREQNKQVRLVVTGANT
                     EVDKLIVEELTDPLMHLVRNAIDHGIESSEGRGRVSKRSEGTLALNAYHKGSNVVIEV
                     EDDGNGMNPHKLRETAIARGLLSPDLGQELTRQDMLNLVFLPGLSTNSEVTDMSGRGV
                     GMDVVKTNILRLGGMVSIASEQGIGTKITVTLPITLAIIRALIVTIASRTFALPITAV
                     REAIPYVPKAVRTIQGREVITLRGETLAICDLSRLFGFRRSNEEQGRFIVVGSMGNRE
                     LGFVVDGLIGQEDVIIKSLGKSLASVRGFAGATDLGDQKVALVLDAPALFAEYFDGPE
                     ALRSLETRP"
     gene            complement(23115..23504)
                     /locus_tag="VFG22_07585"
     CDS             complement(23115..23504)
                     /locus_tag="VFG22_07585"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6416143.1"
                     /translation="MVLHALIVDDSSAVRAFVRASLEDADFARVEEAATGFEALRLLA
                     SNAFDVVIVDVNMPDINGLELLAFMKKSPRQQGARKILISTQVEGRDARRGTELGADA
                     FLEKPFEVDELRELIRGLLSTAAGGAG"
     gene            complement(23555..23896)
                     /locus_tag="VFG22_07590"
     CDS             complement(23555..23896)
                     /locus_tag="VFG22_07590"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HET6416144.1"
                     /translation="MSDAPEPVELRAPRGARVMEIDWADGHQSVYPHELLRGFCPCAH
                     CQGHEGPIQFVEGGDFELSDVQPVGNYAIRLVWRDGHQTGIYSHRFLRSLCACSSCAP
                     DAPKSRSFWRE"
     gene            complement(23893..24651)
                     /gene="recO"
                     /locus_tag="VFG22_07595"
     CDS             complement(23893..24651)
                     /gene="recO"
                     /locus_tag="VFG22_07595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HET6416145.1"
                     /translation="MPSRSRQSRALLLRSIDYRDSDRIVTLLTEDYGKISALARGARK
                     SQRRFGGALQPYVLMNAHFRPGRGELAHLEQVSVDRSFHEILRSLEAIGSAGAAMAVI
                     RERVPDHEPEPAVFDAAVRFLAALNERAPAKEALLCLQIRVLTILGFAPTLDRCVHCG
                     KVPAVGRAASFDAARGGIVCRACGGGRLILSAGALRRWVLIQSTNELPEQPWPERERQ
                     ELDDALGELDAIHATVTVRERTAMASGSWEGRTP"
     gene            complement(24567..25055)
                     /locus_tag="VFG22_07600"
     CDS             complement(24567..25055)
                     /locus_tag="VFG22_07600"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6416146.1"
                     /translation="MRESIGDYLKTERETRRLSLEEVSSTTRIPRKTLESLEHDRFED
                     LPSGVFVRGFIKAYASAVHIDADEVLERFDRQRPKTLPPAPLATVDSRRGYGAVVLAV
                     VTAALFAIIVATFVLVRRAPAQKDPIELSSALPCQVEAARAERSSCDPSTIATRTESS
                     LS"
     gene            complement(25055..25846)
                     /locus_tag="VFG22_07605"
     CDS             complement(25055..25846)
                     /locus_tag="VFG22_07605"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6416147.1"
                     /translation="MLRIWGLVCLACLALACATTSASGTPSGERSFREFQLAASLRDE
                     GQTASAIEHLRMALELDSSNAEAHLLLGFIQMERRDYENAEQHLNAGIKLLEKQKRGG
                     STLAEARNIYGLCLMELDRPDDAIVVLRQSAEDELNTAPHLAWGNLGLAQLNAGEYQE
                     AVTSTMEAVRIQPRFCVGYFTMAQALWHLQQLKDAERALVSALQADPSCAKSPQLQGA
                     WRLRGEVRARLGHRQDAVADLERCVELDPYSNDGRMCQTILEDSR"
     gene            complement(25896..26549)
                     /locus_tag="VFG22_07610"
     CDS             complement(25896..26549)
                     /locus_tag="VFG22_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET6416148.1"
                     /translation="MPESADELEIERYYQSFSAGSTLYYAGAPASELYLIREGRVQLI
                     KRARGVERSVGLFGSQEIVGEEALLPGAHRNATARAIEPVTALVIESDTFRALARRRP
                     DVGDSVMQQLARRLQRAEEQIENFLLPDPTIRVLNSLLRAAESDTAGPLELSPLELST
                     RTALELDQVKAVVGQLKDRGYLSLGDQTITISDPSALRQLHDLLALKEDVRHGLSCR"
     gene            complement(26589..27566)
                     /locus_tag="VFG22_07615"
     CDS             complement(26589..27566)
                     /locus_tag="VFG22_07615"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HET6416149.1"
                     /translation="MSEQTIVRAESLRKYFRGPSRGIGRGHAELRAVDGVSLEVMRGE
                     TLGLVGESGCGKSTLGRLLLRLYEPTSGRIYFEGQDITELSQSALRPFRRKMQIIFQD
                     PYSSLNPRMTVRAALAEALRVHGIVRSGLEEEQRIAALLERVGLRPEQMRRYPHEFSG
                     GQRQRIGIARALAVSPSFIVADEPVSALDVSIQAQIVNLLSDLQVELGLSFLFIAHDL
                     HVVEHVSRRVAVMYLGRIVELARSEQIYSSPRHPYTEALLSAAPEPDPGARKRRLLLE
                     GDVPSPLDPPSGCAFHPRCPISEKGLCDREVPALRSLAEGHAAACHLAR"
     gene            complement(27563..28537)
                     /locus_tag="VFG22_07620"
     CDS             complement(27563..28537)
                     /locus_tag="VFG22_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6416150.1"
                     /translation="MSEPLLSLRHLITSFETDEGYLRAVDDVSFDVFEGRTLGIVGES
                     GCGKSVTSLSIMRLIPSPPGFIEGGEALFDGRDLMQISESEMRRLRGNEISMIFQEPM
                     TSLNPVYTVGSQIVEAIRLHRKMTRREAKARAIELLDLVGIPAPAERVDSYPHTLSGG
                     MRQRAMIAMALACEPRLLIADEPTTALDVTIQAQILDLLRSLQQRLGMSIIFISHDLG
                     VMAEFAQDIAVMYAGRIVERASTNELFRNPRHPYTRGLLRSLPSARNRGQRLPTIRGI
                     VPDLGNLPRGCRFQDRCDLVSEVCRSEEPGLQQLEQSEVACFHAEVSA"
     gene            complement(28578..29390)
                     /locus_tag="VFG22_07625"
     CDS             complement(28578..29390)
                     /locus_tag="VFG22_07625"
                     /inference="COORDINATES: protein motif:HMM:NF025490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HET6416151.1"
                     /translation="MTRACLTALLCSVLSIAGCKSVPACPVQARTDAAKALEDHAQRQ
                     AGWRSIKAEARVTQWGRNGRVRGTVLMFLERPSRVRFDVMTPVGPAAVLTSDGESFQL
                     SDLRAGTFLHGPTCPQNIARLLGISVEAENVLLLLTGDTPLIEAEEQSMECRDGRYLV
                     TLVAADGSTQELAFSIDESDRAKAPGEQRLTLRRSTERGPDGKTRWTATYDEHIEIDG
                     HSFPTNVRFTDEVNDADTSVRVKSIFVDPEVPADAFQQAPGPGMSIEFAPCS"
     gene            complement(29390..>30385)
                     /locus_tag="VFG22_07630"
     CDS             complement(29390..>30385)
                     /locus_tag="VFG22_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416152.1"
                     /translation="AFDIAYRLTDGLPMYEGQALVQSTCGDGAFCHSPAATGTERIGV
                     PAGLNFDVNLACIDDSLDPSCSQPIASCESGQTSTPYCSRLSRLQSNVNTVNDWAEGM
                     IQEIRAGSMPPGAAGESVRNNTPWIRKSDGSELPSIGTKEAEEIIRNWLACEAPVVAR
                     TEIPPSAELELTACASVDDEICIYNGPEGDLPDPAWSDIYWSIMFQSCVICHGPANSN
                     VDQNPDNPIGGAIPGGASPAGLAALNLTGADTTNTASWPSESWSAVVDALAADPGACA
                     GQGTLVIPDDSPGSIMIQKMRGVQTCGDRMPLGGSISESLIQVVEEWIALGAPND"
BASE COUNT         5885 a         9538 c         9218 g         5744 t
ORIGIN      
        1 ccggccttca ctacctgggc ggtctggtga cgctggcgcc agagctgacg gcgctctact
       61 cccccttcgt caacagctac aagcgctatg tcctgggcgt ttgggcgcct ctcaatgctt
      121 cctggggcat cgaaaaccga acctgtggct cgcgagtgat cctcggtccg gacgacgcgg
      181 ctacgcgcat cgaatttcgg cagacggccg ccgacatcaa tccctacatc gccatggccg
      241 cctgcctcgg ctccggtctc tacggcatcg agcacgagat cgatccaccc gcgatgacct
      301 caggagacgc gaccagcgat agcggcccaa gcctcgggct acccctcacg ctggaggcgg
      361 cggtcgagcg gctccgagaa agccaggccg ctcgggccgc actcggagac gacttcgtcg
      421 atcatttcac ccgcactcgc gactgggagt gccgcgagta ccggaaggcc gtgagcgatt
      481 gggagctccg ccgatacttc gaggcagtat aggtaaatag gatgtttaaa cgcttcttgt
      541 cggaacggcc gcttcgtctc ccgcacctca gaccctacgt gcccaggccg agcgatccgt
      601 cgatcttctg gttcaacagc gagcgtgatg acgtcgtctc cgatgtcgtg aagcgaatct
      661 gtgatgatca cgaaacggat cgcgagaggc tcgagatggc gctcaacgac gccgcgttcc
      721 acgagattcg gcgtctcgag aagcagcgtg acgaagaagc gcgcgatcga ctaggctact
      781 ggcgctcgat gctccgccgc atcggcaaga tggatgacgt agaacagcgg cgggtgctgc
      841 acaccatcgt gacgaacatg acgcgcgacg tggcaggcaa cttcgatcct cgcgtctatc
      901 gcttcgcgcg tcgtgcgatt ccgcgtctga tcggcggcgt catggagccg cgaagtctcg
      961 gcaaggagct cgtcggtagc gcctcggatt cgctggatcg cgtgctgaaa gtacagggtg
     1021 atctcgagca tctccgctac ctccagggtg agggaagcct ggtcttcgta ccgacgcatt
     1081 cttcgaacct ggattcgatc gtgctctcgc aggcgctcga aacctctggc ttgcccccgg
     1141 tcgtctacgg ggccggcaag aacctcttca cgaacccgat catcagcttt ttcatgcaca
     1201 acctaggggc ttatcgagtc gatcggagga tccgcgtcag cttatataag gacgtattga
     1261 agttgtactc acaggtgatg atcgagcgtg gctatcactc gcttttcttc ccgggaggca
     1321 cacgaagccg ctcgggaatg atcgagcgtc acttgaagct cggcttgctc ggttcggccg
     1381 tcgaggcctt cacgaccaat cgagttcgac gcgtcgaccg tcctgtctgg ttcgttccga
     1441 ccaccatcaa ctacgcgctg gttctcgaag cagagaccct cgtcaaggac tggctaaccg
     1501 aggagggaca ggcccgatac atcatcgagg acgatgagtt ctcacggatc gatcgctggt
     1561 ttgctttctt tcgaaagatg gtcgggatgc gcggcgcttg catcatccgc ttcggcgagc
     1621 cgatcgatcc ctttggcaat cgtgtggacg ggcgcgggca atcattgacg ccgacaggaa
     1681 ggacgatcga tgcaggggga tacgtcgaac ggcgaggcac tcctgtcgct gatccggcgc
     1741 gagacgcagc gtacacgcgc gagctcggcg aggtgcttgc aaagcgctac gaacaggaca
     1801 ccgttctgat gagcacccag gtcgtagcgc acgttctcta tcgcgatctc gtgcagacga
     1861 caccgggcat ggacctgttc gcgcgccaac gcctccgcgg tgaagtcggt ctcgatcgag
     1921 agagcctctg ccgtgagctt ggagaggcac gcgaccgcct cgtcgcgctc gaagcagaag
     1981 ggcgcgtgag gacgagcgac gtcgtaaagg aagactcgcc ggatgagatg ctggagcggg
     2041 ctctcagctt ttggagcggt taccacgatt cgacggcggc aaccacggaa ggaaaccgca
     2101 tcgtcatcgg ggacccgtcc cttcttctct actaccagaa tcgcctcgtt ccatttgccg
     2161 aagcgatcgc agaccctggt cagcttcggg ccgcctgcga gatcgagaac ctgggggctt
     2221 cccgatgagc cttcgaatcg gagtggtcgg tgcaggcgga ttcggccgcg cgatagcgct
     2281 ggcctcggcg cgccatggcc acgatgtctg gctctggagt cgacgagaac gagagctccc
     2341 ggatccaatt cgcgtgtcca ctgatttgat ggcgcttcgc gaatgcgagc tgctcttctt
     2401 ggcggtgcca tcgcgccacg cagaagaagt cagcgagcaa ctgtgcgaag tggtcgacgg
     2461 acgccacctg atggttcacg tcagccgcgg tctcatcggc gcggagctca agaccatcac
     2521 ccaagtgatt cgtgagaaaa ccgtttgccg ccgcctcggc gcgctcgcag gtccgatcgt
     2581 cgcggaagcc ctggctgaag gtcgaccgag cggagcgatt ttgggaactc gctttcccga
     2641 gctcaccgaa ttggtgcgtg aagcgttggg tggatccgag cttcgaatgt acgagacgcg
     2701 cgacgtcatc ggtgtggagc tcgcgagcgc cacggttggg tttttgacgg tcgtgctggc
     2761 gtatgcgcag gagatcggcg tcgatcccgg aactctcgct gtgctggcca cgcgagggat
     2821 ggtcgaagtg tcccggattg gccaaacgct gggggcagac gagtcgacgt tcatggggct
     2881 cgcgggcctc ggcgacctca ttgccgccgt cgcgggcgat caacgacccg aacagcttct
     2941 cggccgtgcg ctggcgcaag gtgccgactt gagcaatgcg gtcggtaaag cgggtgccta
     3001 catcgagggc atcgaggtcg cccgccattt gatggagcac gcgcggcgcc ttggcctaga
     3061 aactccgatt tcgtccactt tcgtcgctct gtccgagggt cgcatcagcc cggatgatgc
     3121 tatgcatgcg ctcatggaga ggcgcgtggg caaagaatga cggactcgaa ggacctggtt
     3181 atctggagct ttccgactcg ggtgcttctg gggaacggcg cagcggcccg ctgcgcggaa
     3241 gaagcgattc gacttggggc ctcgcgggtg ctgctcgtgt cggacaaagg cgtcgagcga
     3301 gcgggactgc tggactcgat tcggagcgcg ctcgacgcgg ccggactcca acaccagtca
     3361 gccctggaga tctcatcgaa tccgctcgaa gcagaggtgc tcggagcaac gcgtgagttc
     3421 gatcagctca aggctgatct gatcctggcc gtgggcggcg gaagcgtcct cgacgtcgcc
     3481 aagctggttc gtctggcggc cacgcatcca ggtccgctcg cacagtacga cgatgccatc
     3541 ggcggcggcg acaagatcaa cggaccgcta ccgcccatga tcgccattcc cacgacggcg
     3601 ggaaccggca gcgaggtcgg ccgaagcggt gtcgtgacga ttcaatccac gaaccgtaag
     3661 acggtcattt tttcgccctt gctgctgcct gacgtggcga ttctcgaccc tgaaatgagc
     3721 cggaccatgc ctcccagcat gacggctgcg acggggatgg atgcgctcac ccattgcatc
     3781 gaagcctacg catcgatcgg tgatcacccc atggccgatg cgatcgcgct aggcggagtt
     3841 cgtctttgcc aacagtcact caaagcggcg acagacgacg gcgacgactt gaaggcgcgc
     3901 gcggacatgc tgaaggccgc aatgatggga gccgttgcat tccagaaggg gctcggagct
     3961 tgtcattcac tcgcccatcc cttgtctgcg gagttcggaa tgcatcacgg cctggcaaac
     4021 gcgatttgtc tgcccgccgt cctcgacttc aatcgtgcag ccgtgcctga gcgcattgcg
     4081 gaggtcgcgc gtgagctcgg agtgcgcggc aacgacgaga gcacactgtc gttcgaatgc
     4141 gccggcgcaa ttcgggcgct tcgaaagtcg atcgggctgc cttcgaacct gcgatcgcag
     4201 ggtgtcgagg agagcgactt gccgcgcctc gcagcgctag cctttgccga ctcctgtcac
     4261 cagctcaacc cacgccgctg caccgaagac gacctgctcg ccctctacaa ggccgccctc
     4321 gacgatgact aagcgaaaaa aggggcaact cgaatcgatc ggcaagctgc tgaaaggcgt
     4381 ctaccctgcc ccagaacaac tcgaggcagc gaaggtgttt gcttggtgga gccgctcggt
     4441 tccgccacga atcctcgagc atgcacgccc ggtgcgactc tcccggggcg tgctcatcgt
     4501 gcacgttagc tcctcggtct gggccaacga gcttcactat ctcagcgacg atcttctcac
     4561 acgaatccgc gaggccgcgc ccacgaccgg catcgagaga attcgttttc aagtgggccc
     4621 ccttcccgac atccccaaac acgcgcagcc cgcaccggcg ccaccgcagc ccattcgact
     4681 cgcgtccttg cccgaggagc tagggcgcgc gctgagtcga gtcgaagatg acgccctgag
     4741 aacagcgatt gcagacgcgg cgtccacgag cctcgcgaga atcagggagc cgaaggacta
     4801 ggccggagtc gctgacgctg gcactcggct cagcgggttt gtcttgctgc gcgtatcatg
     4861 gcttcgagcg tttccgctcc gtctgcgctt gcaagttggc tcgagacatc gtctggtagg
     4921 ggcacggtcg tcgcgccgag cttggactcc gtgagcacat gaagagtcac gcccagctcg
     4981 cctgcgatgc gtttcatcag gtcgacgcct gggtcggcga cttccgcgtt ttcgaggcgg
     5041 gcgatcgttc gccgggaaac acctacgcgg tcggcaagct cgagctgacc caggcggctc
     5101 ctcagctcgc ggaggatcgg aaggttggcg gatatgacat caatagtggc cattatgaga
     5161 cactatcgtc tgctttaggt catggcaagg ccgctactgt ctggtcaggc cccgcgccag
     5221 gtcggtgacc agcgtccgaa cggcggcaac cgcttcatcg ctgctcgaag cctcctcgat
     5281 ggcctggacg acagcactgc ccaccaccac gccatccacg ttcggggcca ccattcgcac
     5341 gtcctccttg gtcttcactc caaagccgag cgccagaggc cgaccgagct cgcgttgcaa
     5401 gacgccggct cgcttcgacg cgttcaccaa gtccgccgct tgggagccgg ttacgcccgt
     5461 catcgagacg tagtagatga aggaggtagc tgcgtccccc gcctgtcgga tgcgagccgg
     5521 atcgctcgta ggcgcgatca gcgggacgaa gtcgagcccc ttggcgacgg ctggctccct
     5581 cagcgcggcg ctctcctcgg gaggcagatc gacaatcaga aagccgtcgg ctccgctttg
     5641 ggctgcatcg tcgacgagtt tccgctcgcc gtaggctaga acgggattgt agtaaccgaa
     5701 gagcaagatc ggcacatcgg ttcggccgcg aatcacgcgc acggtgtcga gcaccttctt
     5761 gacgttggtc ccaccgcgca gtgagcgctc ggcggcccgc tgaatcgccg gaccatcggc
     5821 agttgggtcg ctgaagggaa caccaagctc gatcacgtcc gcaccagctt cggcggctgc
     5881 gactacgagg tcgaccgttg tgccgaggtc tgggtcgccc gcgcaaaggt agatgacgag
     5941 cgccgcgcgc ttctcgtttc tggcgttttc gaaggcgtcg gcaatccggc ccacttcagt
     6001 tcgccctttc cttcagcttt tcgagcactg tctcgaggtc cttgtcaccc cggcccgaga
     6061 ggcagaccag gatgtccgcg tcgggaccga gctccttggc aagcgtgggc agatacgcaa
     6121 aggcatggct cgtttcgagc gctgggatga tcccttcgat cctgcagagc tgctggaacg
     6181 catcgagcgc agcgtcatcg gtcacggcgg tgtacgtggc gcggccggta tccttgagcc
     6241 atgcgtgctc gggtccgacc ccggggtagt cgaggccggc gctgatggaa tgcgcttcga
     6301 cgatttgccc atcggcggtc tgaagcacgt agctcttggc gccatgcaat acaccgacgc
     6361 ctcctgcgca gaggggggcc gcatggtgcc cggtttcaat cccctcgcct gcagcctcga
     6421 cgccgatcag acgaacggat gggtcgtcga tgaagcccgc gaagatcccc atcgcgttgg
     6481 agccgccgcc cacgcatgca acgacggcat cgggaagcct cccggcttgc ttgagcatct
     6541 gcgcccgcgc ttcctggccg atcacggact gatagtcgcg aacgatcatc ggatacggat
     6601 ggggccctgc caccgagcct acgcagtagt gcgtcgtgcg aacgttcgtg acccagtcgc
     6661 ggagcgcttc attcattgcg tccttcaaag tcgaagaccc cgacgtgacc gagtgcacct
     6721 gagcgccaag caactccatc cgccgcacgt tgggcgcctg tcgacgtacg tcttcggccc
     6781 ccatgtagac ttcgcagggc agccccatca gcgcacacgc ggtcgcggta gccacaccat
     6841 gctgacccgc cccggtttcg gcgatgatcc gggtcttgcc catccgtttg gccagcagca
     6901 cttgcccaat cgtgttgtta accttgtgcg cgccggtgtg acatagatcc tcgcgcttga
     6961 gccagatccg agctcccacc ttctcagaga gccgagaggc gaaatacgta ggcgtctcgc
     7021 gccctgcgta ctggtgcagc agcgagtcga gctcggcctg gaactcggga tctcgttgcg
     7081 attcctggta ggcctgcgtc agctcgtcta gcgccggcat cagggtctca gcgacgtagc
     7141 gtccgccgta tggaccgtag cgcccagcag ttggttcggg gggttgcatg agacgtggaa
     7201 cgtagcgcat agaagaccct cgcgcatgca catgactcag ccaagcaaca cgaccattcc
     7261 accactaaat cgagaggcct atgagcgcat gctgcagcgc acatcattct tgagcgaatg
     7321 gtttcgaccg agcatcgaag gtctcgaaaa catcccgacg aaggaaggcg cgctgctcgt
     7381 gaccaaccac ggtcacttcg gcatcgacct tcccgttctc ctccgcctca tcctagaagg
     7441 gaccgggcgt gcggttcggt cgctcggaga ccggatcgtc ttcgccaccc cattctttcg
     7501 cgatatcgca cacacgatgg gcgccatcga gggcgaaccc gaaacgactg tgcacttgtt
     7561 ggaagacgac cagcttgtgc ttgtctaccc gggcggcgct agggaggccc tcggcgatcc
     7621 caaggatgcg taccggctgc aatgggagca aagtcggggc ttcatccgca cggcgctacg
     7681 cgcacaaaga cccatcgtcc cggtggccgg gctcggcaac gaggagctct acgtgcaagt
     7741 ggttccgcag gaccgtgtgc gaaagagcgg catcggccga ctcattaccc agctgctcgg
     7801 tgataagtac gtcatgccca tctacatggg cttgggcgct ctcccgttcc caacggagct
     7861 gcactacatc atcggaaagc cgattcacct tccgtatgga cccgaggccg ctgatgatcc
     7921 ggaggtcgtc gctgggcttc accatgaggt caccgagacg acccagcgct tgatcgacgg
     7981 aggtttggat gcccgaaagc aactcggttt ggcggacatc ggctagtcag ggtgcttcac
     8041 cgcgcctctc tccacatcag ccacatgggc ggtccgccca tggccaatcg aatctcctcg
     8101 acgatctcga acccatgcgc ccggtagaag ggtaagttgt tcacgttcga gctttcgaga
     8161 taggccggca tccggtcctc gtcgcatcgt tccaaacccg ctcgcatcag cgcgcctcct
     8221 acgccgcgtg ctcggtgcgc ttggcgcgta ccgatcgcga agagatagta gaagtcctcc
     8281 ctgggatgcc gcttctgtgt ttggatcaag gtggcaaacg cgcgcaagag actccgaggt
     8341 ccataccgcg tcagaccgtc ccagacgacc gacggactca ctggggactc gagtgccacg
     8401 ccgggtggaa gccaggaagc agcgcccgaa ccgtcctcgg ccaaatgagt gagaccatgt
     8461 cctagacaaa acggaacggt cagactgaac acgtacctcg gatagttcgg gttgcgagtg
     8521 acccatctca tgacggggtc catttcaaag gcatcggcca gaatgtccag cacagcgtcc
     8581 aagtcgctcg aatcagctcg gcgaatccga cttgtgtctg gtaaatgcat cctgacccgg
     8641 tatagtcgca gagcgggcct caggtgaagt acaagctttc tcgacgaacg tttacgaagc
     8701 tcggtgtgct aagcggcgct agcgcgttag tcctcccggg gtgcagcgac gaatcggtaa
     8761 tcacggaggg gtccgccaaa ctgactgctg acgagcgaga gctcttggag cgcttcgcgg
     8821 aggtttacct cccgaccgag gggacctctc tcaagccgcg ctcggaagtt cccgtcgtcg
     8881 ataacatcga gcacgcgttc tcgctcatgg acgccccgac cctcgaacag gtgcgcattg
     8941 ggctcaagtt gttcaacctg ggctcgatcc tgatcgggct ccacttcgcc cgtttcactc
     9001 acctctccgc ggaacaccgg ctcgcctacg tgcgaaaatg ggaggaaggc gtcgaggttc
     9061 aacgcgggat ctcgaccctt ctcaagaaac tggtctgcta cggctattgg aaagacatag
     9121 aagccggtcg cgcaatcggc taccagggtc cagtctcaga ggccagcggc atcgtaagcc
     9181 ttggtaatgc gccgatgccg gcaaccccga gctagggagc acactcgtgg acgaatcaaa
     9241 tccggctatt caagagcgtc cggtgctgaa ggtgaccgcg ccaagcgcga ttccggatgc
     9301 cggtctcacg ctggtagccg atgtcgttgt gatcggttcc ggagcagcag gggcggtgac
     9361 cgcgtacgag cttgctcgca gcggcgcgaa ggtcgtggtc ttggaggctg gtccacacat
     9421 cccgtcgagc gacttcaagg agcacgcgcc aaccgcgctc gaaaccttgt acgaggacca
     9481 cgggaaccag gtgaacacca cgggtgacct gacgatactc cagggcaggt gcgtgggggg
     9541 ctcgacggtc gtcaatgcgg ccgcgtgttt ccgtgtaccc gatcaagtcc tcgagcactg
     9601 gactacggaa catggcctcg acaatctctc tcccgatgct ttggcgcctt acttcgagaa
     9661 ggtggagcgg aacctctcga ttcgccaaaa cgctgcccac gaggtcaact acaatggacg
     9721 cctcctgatc gaaggtgcgc agaaactcgg tattccggca tctccgattt cccggaacgt
     9781 caaggactgc gcgctcagcg gccactgcat ctccggctgc aagacggatc gcaaacagtc
     9841 gatgctggtc acgtatctac cctgggcaag tgagctcggc gtcacgatcc tgagcggcac
     9901 acgtgctgaa acgttccaaa cgagcggtcg tcgcgttacc tcggtaaatg ctgtcgccac
     9961 cgatgaaaac ggaaaccaga aacccgtgcg ggtggaagcg gggctcgtcc tggtcgcggc
    10021 tggcgccgtc cagactcctc tcctttttca gaagaacgcc ctcgccaaca gcagcggcca
    10081 aatcggcctc aacttcgcgt gccatccgtc actcgcggtc tttggagagc acgaggggga
    10141 cgtttttgct tgggtcggcg caaccttgac cgcgcaagcg ggcgacatcg agaaccccct
    10201 caaaggtggt tacctgctgg aagcgggaat ggtgggaccc gtcgtcacca gcagttggat
    10261 cgacggaggg ctcggccggg acttcgcctc gttcatggag aacagcaaga agtatcaggc
    10321 agcggtcacg ctgatccacg accacaacgt cggtcgcgtg tacctcgagg gtgatcgcaa
    10381 acgcatcgag tacgacctcg acgaccgcga cttcgaatcg atgcgcgagg ccctgcgcgg
    10441 aactgcgcga atctatttcg ccgccggagc ccgtcgggtg tacctgccca cgactcgccg
    10501 gacgaccatc gaaagcgctg agcagatcga ctccgtgatc gatcggctcg ccaacggaaa
    10561 gcacatgtac cgaatgaact cataccatcc gcagggtacg atgcgaatgg gcgcggaccc
    10621 ggcgaagagc gtcgtcgggc cggacggtcg gtgccacgac ctcgacaacg tctatgtgcc
    10681 ggatgccagc ctcttccctt cgtcgctctt ggtgaacccg caggtcaccg tgtacacgat
    10741 ggcgagctac atctccgacc aaatccttgc cagagcttga ttactgagcg ccgcgaattc
    10801 ggctgaagca ccagaacccg gcatcggcct tggcgagacg ccatccatcc tcgccgaggg
    10861 cgctgaactg ggcactcatc tcctcggccg ccttcttgct ggcgcgctcg ctctgatgga
    10921 cacacttgta ctcatgcttg tgcgcagcgt gccaaccatg gtgggggccg cttttgcagt
    10981 gagggcatgc cttgccttga tcgcagtggg gacacgcttg tccttttgcg cagtggggac
    11041 aggatgttcc cgcggcgccc tggccgcagg tctgacagct agcgggcttg gcctgttctt
    11101 gagattgagc cgggttgcca ccgagcgcga tccacatcgc ggccgcggtg aaaagagaag
    11161 ccagaatggt gagacgcatg tttcctcctt gagttgaggc cgggacccta ccacgaacaa
    11221 ttcacgcttc gtaaaaggcg gactgtcgtc gatgctggag aagccgttat gcgccgcctc
    11281 gtgcccatga accgcctatg gcgaattttt ggcggctcga atgaaggctc gaaccaggga
    11341 gggatccttg cgaccggggg cagactctac gcctgaggcg acgtcgacct gctgagggtt
    11401 cacggctcga atcgcgtcgg cgacattgct gggcgtgagg cccccagcga ggatgagctt
    11461 gcgttccttg gcaacctcgg cggcgatctt ccagtcgaag gttctgcccg tcccgcctgg
    11521 caatcctgga acgctggcat cgaggagcag atgctcgccc ggatagctcc gggccagctc
    11581 gatcgcggag ccgtctttca cgcctatcgc cttgtacgcg cgtggcagga gcgcagcgac
    11641 caaatccggt ggctcgcttc catggagttg agcccattcg atgcccgtct cggcgaggat
    11701 gtctttgatc tgggcgagat cgaggtcgac gaatacgccg accatccggg catcatcccg
    11761 ggcagccgcc acgatccttc tcgcgacggc caggtcgacg aatctcggag acctcggcca
    11821 gaagttgagg ccgatcgcat cggcgcccgc ctcgatacaa cgcaatgtgt cgtcaactgt
    11881 ggtcaaaccg cagaccttga cctcgaccat gctccgagga tgggccgggg ctgtccgctc
    11941 gtccaacctc cggcgattca cttgcgtctc tgaacctccg gctctagaac gcgttccagt
    12001 ttagccggca agaacgggcg ctcgggcccc tgccggacta gaatcgacga tcgagcatgg
    12061 aaacatctgc cgccgcgcgc gttcggccca gtccgcgtga aaccttggac tccgttgtca
    12121 ttcgctttgc cggcgactcc ggcgatggaa tgcaactcac agggagtcag ttcacgactg
    12181 ccaccgccga cgcggggaac gacctttcga cgtttcccga ttttccggcc gagattcgtg
    12241 cgcccgccgg aactacctac ggcgtctcgg gctatcagat tcatttcgca tcgcacgacg
    12301 tgcagacacc gggggacgca cccgacgtcc tcgtcgcgat gaacccagcc gcgctcatgg
    12361 tcaacagcga agcgctaaag cccgggggcc tggtcgtgat caacacggga gcgttcacgg
    12421 cgaacaatct caagaaggcc ggatacgaga acaatcctct cgaagacggg tccctcgatc
    12481 gcttccgtgt ccttgcactc gacgtcacca agatgacgct cgatgccgtc caggaggtcg
    12541 gtctgggatc aaaggacgca ggtcgctgca agaacatgtg gaccttgggt ctcatgtatt
    12601 ggctgttcgg acgcgagcga gatcagacgg tatcctggct caagaagaag ttcgcgaaga
    12661 cgccgaagtt ggccgaagca aacattgcag cactcaatgc aggacatatt tacggcgaaa
    12721 acgccgaatt accgcacgga atcaacgctt acgacgtgcc tgcagcgaag cttagccccg
    12781 gcgaataccg caacatcagc ggcaacgagg cgaccgcctg gggtctggtc accggcgcgc
    12841 ggctcgcggg cctcgagctc atgtacggtt cctacccgat cacgccggcg tcgaatctct
    12901 tgcacaatct ggcgcgtctc aagcatttcg gcgtgacgac cttccaagct gaggacgaaa
    12961 tcgctgcggt ttcggccgcg gtgggtgcga gcttcggtgg ctcgattggt gtcaccggca
    13021 cctcgggtcc cggcgttgcc ttgaaatccg aagcgatcgg gttggcaatc gccgccgagc
    13081 ttcctttggt catcgtcgac gtacagcgtg gcgggccgtc gaccggcctg ccgacgaaga
    13141 ccgaacaatc cgacctgttc caggcagtcg tgggacgcaa cggcgatgcg ccactggccg
    13201 tcttggccgc agcaacgccc ggcgattgct actacatggc gatcgaggcc gttcgcatgg
    13261 cggtgaagta catgactccg gtcattctgc tcaccgacgg gtttgtggca aacagcgcag
    13321 agccttggct ccttcccgac ctcgactcgt tacgagcgtt cccggtccgc ttccacaaag
    13381 acccggaagg gtttcatccg ttcttgcgcg acgcgcagac ccttgcgcga gtctgggcca
    13441 ttcctgggac tcccggcctc gagcaccgca tcggcggcct ggagaagcat cgggacaccg
    13501 gacacatctc ttacgacccg cagaaccatc accgaatgac caagatccgg caccaaaaga
    13561 tcgccggcat cgccagggat atcgagcagg cgaaggtcga ccagggcgag tctagcggag
    13621 acctgctcgt cgtcgggtgg ggctcgacct acggctcgat aagccaggcc gtgaagctgc
    13681 ttcgggaaag gggccaccag gtttctcact tgcaccttcg taacatctgg ccgcttccag
    13741 aaggccttgg agagctgctt ggatcattca acagcgtcct cgttccggag ctcaacttcg
    13801 gtcagttggt gaaactactc cgcgccgaat tcctagtcga cgccgagagc ctcacccgag
    13861 tcactggaca gccatttcga attggcgacc taatcgacgc cgtggaagag cggctgcaac
    13921 ggagttcgcg atgagtccca atccgcccaa gaaggaatac gatcggaaag atttcacgtc
    13981 cgatcaagag gttcgttggt gtcccggatg cggcgactac gcgatcctcg cagccgtgca
    14041 gcgcactttg ccgagccttg gctcgacacc ggagaacacc gtgttcatct ccggaatcgg
    14101 ttgctcgagc cggtttccct actacatgaa cacctacgga tttcatacga tccacggccg
    14161 agcgcccgcg atcgcgaccg gcctcaaact cgcgaacccg aagctcgacg tctgggtcat
    14221 cacgggtgat ggcgacggat tgagcatcgg cgggaaccat ttgttgcacg tgctgcggcg
    14281 caacatcgac ctgcaaattc tgttgttcaa taatcagatc tacggtctca ccaagggcca
    14341 gtactcaccc acctcgcagg tcggcaaagt caccccatcg agtccgctcg gttcgatcga
    14401 ccaaccggtg gtaccggcgc gtttcgccct gggcgccggc gcgacgttcg tggcgagggc
    14461 attcgatacc tcgaagaaac tccccgaggc tctgaccgca gcgcgggagc accggggcgc
    14521 ctcgttcgtc gagattcttc aaaactgccc cgtgttcaac gatggcgtct tcgagctgat
    14581 caccgatcga aagtccggtc ccgcctacca actctggttg gagcacggaa agcctttgaa
    14641 atttggcgca aacggcgaga aaggcctgcg attgaaccca cgcacgctga acctcgaggt
    14701 cgtgacggtc ggcgaagacg gcgtcaccga agacgacatc atcattcatg acgagaccaa
    14761 tgcttccctc gcctggatgc tcaccgacct gccagagatc gtcgcgctcg gtgttctcta
    14821 ccgccatgag ggcccggtct acaacgaaag cgtggcgaag cagcgcgagc tcgcagagaa
    14881 acgcttcggg gaaccggact tacacgccct tcttcgggag ggacacacct ggaccgtcac
    14941 ctgaggcccg agcttcggcc accggcaagg tgagaacgat gcgaaggccg tgtggttggc
    15001 gcacggtggc ggtgatgaag ccatccgcgc gagatgccag gtctgccagc ttggtgagcg
    15061 ccagttcgac cttgggccga cgatcgatct tcacgaaggg cgtgaacaag gtgcgaagct
    15121 cctcactaga tgggctcgct ccgtcatctt cgatggtcag tgcagcggcc gacccgagca
    15181 ccctggtggc gatgcgaatg gaagtgacgg tcttggtggc gaccatgaat cgcagcagac
    15241 tcgacaacgc atcctgggcc agccagagat cgagtacgac caccggatcg tcgacgctga
    15301 cgtccagctc cacagcggag ccaaggccgg aaaccacgtt gctcaagagg tcgttcagcg
    15361 acagagctct tgcacggggc gactgactgc tcgccaagat cgacaggttt cgcatcagct
    15421 cgcgaccttc ttcggcggcg tcccggacct ctccgacgta cttctggagc gcctcgacgt
    15481 ccccgagatt cttcttggcg agatgcgcgt tcccgatgat caccccgaga atgttgttca
    15541 cctcgtgaac gatgcctgcg gcgaggtctc tcgctgcccg cattccttct ggatgtaaga
    15601 tgccttctcc ggacgcgtct cggtcgtcgt cgtccatcgg cctcagatct ccaaaagctt
    15661 cttgaccgca cgaatgacgc gcggtccttg aatgggtttc tcgatgtaag cgtttgctcc
    15721 caaggccatc gcacgatctc gatcgttcgg tgcgctctcc gtggtgatga tcacgatggg
    15781 cgttttctcg tgacgcggat cgcttcgaac atgcctcacg agcttgagac catccatcac
    15841 cggcatgttg acgtcagtca cgaccagatc gaaacgatgc gccgccagcg cgcgaatccc
    15901 agtcagtccg ttctcggctt cgacgatctc cataccggga attctcaata gcgactccgc
    15961 aagcatgttg cgcatcgtct tggagtcttc gactacgagg catcggtagc gacgcatggt
    16021 tatctcaatc gtacgacagg acgcggcgtg ctcaaagttc gagcctcatc ccttggttgg
    16081 ccgggtgagc tccgcgatca tgtcgccgag agcccaaatc ccgagtgagt ggtgccgcac
    16141 cccggaccgt cgagcagcca gtggcattcc agcaacgacc gccgtctccg gcgcttcgat
    16201 gaggatcgcc ccgccacggt cggcgacgtc gcggactccc gccgcgccat cgtcccccat
    16261 ccctgtcaga acgacggcga tcgccttgtc accgagcaca cgagcagccg agtgaaagag
    16321 ttgattgacc gacgggacat aatgcgcccg aggatcgggc gctacgacgc ggagggctgg
    16381 acctcggtcc gatgggacga tctcgatgca ccggcctccg ggacaaacgt aggcgtgtcc
    16441 gcgagctagc cgctcgtact gcttcgcttc ggacacgtgg atcactcccg tgcgatcgag
    16501 acggctggca aacgtagtgg tgaagcgcgg aggcatgtgt tgcgcaatca cgaacgctgc
    16561 gtcgatttcc tcgtgcaggt gccggaacaa cgtgagcagc gccttgggac cgccagtcga
    16621 agcgccgatg cagatgacgt tgctcggcac gtgcttcagc acggaaggtt ctcgaaatcg
    16681 gttggctgaa gttcccgctc cggattcttc gtcgagcgcc aaccccccga ccgcggtacg
    16741 acgaactgaa aggggtgtca gctgttgaat gacccgaacc ttctcgacga gttgacgccg
    16801 aaggctctcg agtggtgcgt cttctccttc gggtttggcg agaaagtcga tcgccccaag
    16861 ctccagtgca cgaaagatgt tctctttcgc cgaaagccca gaaactacga ccacgggcac
    16921 cggatttcga tccatcaaga gctgaaggaa gtcgaagccg tcgagacgcg gcatctccag
    16981 gtcgagcgtg atgaaatccg gtggattgga ttctacggct cggagcgctt cggcaccgtc
    17041 gttcgcgaca tccacactcg aaacgcctgg aatctcccgg aggattgcgg cgatcgttcg
    17101 ccgattgagt gacgaatcgt ccacgaccag tgcgcggaga tcgcccacct agcccctcct
    17161 cggaggcaag tccgtgtaag ggatgggtcg ccgatagacg atgtcggtgg tcaactgggc
    17221 gatctcgaaa gccgtgctgc tttggagcag tgattcgctg tgaccaagga gtagatatcc
    17281 accgcggttg agccgttcgt aaatcgtgtc gagcacctgg cgccgggacg cgtcgtcgaa
    17341 atagatcaga acgtttcggc agaatgtcac gtctacccga cccacgattg cggcgcgccg
    17401 ctggttcatc aggttgaggt gcccaaagtg gcacatcgag cgaatgcgcg gatgcacctg
    17461 cttgccctcc gcagtatcga tgaaataacg atcgtactcg ggaatcatcg ctcgaaagct
    17521 ggcagacgta tatgtagctc cacgtgcctt gcgaagtacg cgacgggaga tgtcgttgcc
    17581 gaagacgcga acgtcccatc cctcgaaaag ccccgtcgca tcgacgagca tcgcaatcgt
    17641 gtagacttct tcgccactcg aacagcccgc actccaaacg tcgagccgct tcgaaccgcg
    17701 cgcaagagcg cggctcctca ctacgggaag gatctcgtcc gtgaaggccc gaagctggta
    17761 cgcctcgcga aagaaatacg tttcgttggt ggtcaagacc tcggccgcgg cctcgagctc
    17821 cgcctgagcc ttgggatggt actgcaagta ccggtaatac tcagcgaagt cgtcgatgcc
    17881 gaggtcacgg agacgctctc tgagtcgtcg ttcgacgatg tacgctgaat cgtctgcgaa
    17941 gtagatcccg cagaacttgt tgacgaagtc gcggaggagg cgaaactcgt tcaggttcag
    18001 gctcggcccg gcatcgtgca acaaggtcaa gtcagtctcc gagccgcagc ccgctttcga
    18061 tcgcgtgacg aaccatcgaa tcgctctcga tggaaagcgc gtcggtcagg agtttgcggg
    18121 tgcgtttggt accgatgtcc agcagcaact tgatcgctag catccgaacg ttccaagccg
    18181 catgttgaag gccccgtgcg aggaagccct ctgcatccgt cgctggtaga gtgctagccg
    18241 cccggagcgc ggcctgaaat acctcgtggt ctgaatggga aagtgcccgc tcaacggccg
    18301 cttcgacctc ggaatctccc agccatgcga gcgaatccaa agccgcggcg atcacgactg
    18361 gctccgggtc ctctagaagg acgcggatct ggggaatcgc cgtgctggcg tcccgccgac
    18421 cgagagcgag cgcagcctct gcgcggatcg gagggaacgg agagtccagc gccgtgcgaa
    18481 gtggcgcgtt ggcctcgctg gtgcgcaact gtccgagtgt gcgcacagcc gcaatctgaa
    18541 cgtcgatgtc ttcatctgct acggcatagc cgatcagctg agcgacatcg gttgcgtccg
    18601 ccatcgccag ggcctgcacg accgccctgc gtgtgcgtgg atcgccgctg tgcaggccgc
    18661 gcttgagcac ctcgaccgcg ccgttggacg ctagacgtga caaaacttgc gcgatgtcgg
    18721 cgccgccggg tccatctaga tccgctcgcg cgagggcggc ctcgacgaga tcgggagcgc
    18781 gctgggcaat cgcctcgaga gcgctggtcg cggcagcacg cacttcgtga gaggagctgt
    18841 tgagacattc gacgagcgcg cggcaatctc gcgcgtcacc ccaccagcgg aggctctccg
    18901 cagccgcagc acgtaccgcg tccgttgcat cggtcaggtc gcgctcgatg aggcttcgta
    18961 tgcgatcgcg agtaccgggc ggaacttcgt cgagatgtcg tgagctcagc tcggccgcca
    19021 actcgagagc aatcgcccaa acccgcggct cgccgcggcg gtgcatcaac aatggttcga
    19081 cgaggctcgg tccccagctc cgaagagctt cgacggtttc aggattggcg acgtcgtcgg
    19141 ctacggcttg gatgaccgct tcgattgagg tttcatctct gcatagcacc gacaaatagg
    19201 cggcactctg ccgagtcgcc gtgtccccgt gcaagagagc ttcatggagt gcgtgtcgaa
    19261 acgattcgtt cgaggcaccc agacgttcga ccaactcagc ctgctggtcc tcggcggctt
    19321 ctgcgagctc cccaattgca cggagggctg cagcggatgt ttgaggcgaa gtatcagtcg
    19381 caagctggat taggaactcg agcgcttctg gagccccggt tcttccgagg agctccgtac
    19441 agatgcgacg cacgatcgtg tcttccaccg cgggcttgag ctcttcccag gccactcgcg
    19501 tgcctagccg tgtcagccct tcgacgatcg agatccgtag gaagaggtgc ggcgtgcaca
    19561 aaccctcttt tagaagtgcc tccgctcgcg cccctccaat gcagaccagt gcttcgaccg
    19621 cggccgcagc gacgttggcg tccgagccac gcacgagcag ctcgaggtga tcgatcatct
    19681 gagggtcacg agtcttcccc atcgcttcta cgagaaactt cttcgctccc tccccggctc
    19741 ccgcgcacgc ctcggcgaat ttctgggatt ggccctgagc acacaagcca agcacctcga
    19801 tggccgcgtt ccgcagaccg atgttgtctg tctcgaccaa gagggccaag agcgcgtcga
    19861 tcaacgcgct cttctcgacc gtctgggccg ccagagaaat ggcctccctc cgaacgcgcc
    19921 aatctcggtc ggacagcgcg tcgaggatct gcttgagggg tgtaccctga ggacgaaggc
    19981 tcatctggta gacggcctgc cgccggacgt ccggatcggc agaatgaaac gcgtcttcga
    20041 gtctgtccga ggtcacggcg cctccgatgg caacgagggc agctcgcctc gaggtatctc
    20101 catggcaggc tcgaccagtc gactcgcgtc gagcaggaac accaactgcc ggtcgtgtcg
    20161 gaaggccgat gcaatgcctc gctcccggtg acgttcgtcg agaacgggaa cctcacgaga
    20221 cttctcttcg gctgatgaaa acacgtcgag gacagcgtcg acgacgacgc ctacgagctt
    20281 tccatccgat tcgatgatga tccacttggt gcgtcgattc tcctcctgcg caggcagtcc
    20341 gaacagccgt cgtaaatcga cgatcgctac gacgctctcg cggtagtccg aaaccccgat
    20401 gacgaaaggc cgctcatgcg gaagcgcgat cagcgggagt gggttcacta tctcccgtat
    20461 tctggcgact tcgagcgcat acatgacgcc gttgacttcg actcgaatga ggtctttggc
    20521 cagcgttgcg ctaaatgctc ttcctgccct cacgtcatcc tcccagcaag gacacggggt
    20581 caatcaaaat cagcatgtcg tcagtcgcgg taggcccgct cgaggacata cgcccgattc
    20641 cgatgacgta ctccgacagc tcggcaccca tggcgctggc gtactcgatc tgcgcgggtt
    20701 ccattcgata gacctggatg acttcgtcga ccgcaacccc gatgagctcc tccccgttgt
    20761 cgacaaggag cacgcgtcca caagagcgtc gctccgcgac cgcgaggctc aacagctttg
    20821 gcagatcgat caaggtgatg atttggccgc gaaccgagat gacacccagg atgtgctcag
    20881 ctgtccttgg aacctcggca actggaggcg ggacgagaat ctcacgcact gaagacaggg
    20941 ggaaaccata cgcttcgtcg ccagccaaga cggccaaaac ggcgaccgca ccgcccttgc
    21001 tatgcgggtc gggtacgaga gttgcttttt gcgccctcga gctcatggcc gcgtctccaa
    21061 agacctcagt gcctcggggc cgtcgaagta ctccgcaaag agagcaggcg cgtcgagcac
    21121 cagggcgact ttctggtcgc cgaggtcagt ggcccctgcg aacccacgaa cggatgcgag
    21181 gctcttccca agcgacttga tgatgacgtc ctcctgtccg atgagtccgt ccactacgaa
    21241 gcccagctca cgattgccca tcgaaccgac gacgatgaaa cgcccctgct cctcgttgga
    21301 gcgccgaaaa ccgaagaggc gtgagagatc gcaaatcgca agtgtctcgc cccgcaaagt
    21361 gatcacctca cgcccttgga tggtgcggac tgctttcgga acgtacggaa tcgcttcgcg
    21421 gacagccgtg atggggaggg caaacgttcg ggaagcgatt gtgacgatga gcgctcgaat
    21481 aatcgccaac gtgatgggca gcgtgacggt gatcttcgtt ccgattccct gctcgctggc
    21541 aatcgagacc attccgccta ggcgaagaat gttcgtcttc accacgtcca tgccgacgcc
    21601 cctgcccgac atgtcggtga cttcagagtt tgtgctgagt cccggaagga agaccaggtt
    21661 cagcatgtcc tgacgcgtca gttcctggcc taggtcaggg ctcagcagac ctcgcgcgat
    21721 tgcggtctcg cgaagcttgt gcggattcat gccgtttcca tcgtcttcga cctctatgac
    21781 gacgttgctg cccttgtgat acgcgttgag cgctagcgta ccctcggacc tcttggatac
    21841 acgaccgcgt ccctcagacg actcgatccc atgatcgatg gcgtttctaa ccaagtgcat
    21901 cagtggatcg gtcagctcct cgacgatcag cttgtcgacc tcggtgttcg cacctgtcac
    21961 cacgagtcgc acctgcttgt tttgttcgcg ggcggcctgt cggacgatgc gagcaagctt
    22021 gttgaagacc tggcccagcg gaaccattcg gacgtcgagt attcccgaac gaagctcagc
    22081 cagctggcgt tcgaatccgc gaccgatgcg gtccaagctc atcgccgatt gcctgtccag
    22141 cgattcgcgc ttgagctcat cgacgacccg accgatcgcg ctacgaacca atgcaagctc
    22201 cccaacgaca ttcatcaagt gatccagctt gcgaatgtcc acgcgaactg aattagccac
    22261 ggagcgcagc gacgcctgac tcgcatcttc gtcctggcgt ggcccgatgc ttgtagccat
    22321 ggagggcggc atcgacgtga gatgcggggg tagcgtggga aggtgggttt gctccgggat
    22381 cgcgtcgatc gttccgtatc gcccttcgaa atgattcttc agctcctgct cggggactcg
    22441 actcgcaagc aggactacca actcgatggt ctcgttgttt ccgccctcca tgctgggcag
    22501 ataggtgatg atctctgcat catcccgcgc gacttcgcgg agggtctcaa tcgagtcatc
    22561 aatggcagtc aagtcgaacg cggcgcgaag gcggtagagt cgaaggcctt cgcgaatgca
    22621 cgcgcgaagc cggtgttctt cgtactcggt gagaacggaa agcacggctg gatcgagcgc
    22681 gtacctttcg agtaggtcgc cctcctcatc agagggcatc atcccgatcg accgaatggc
    22741 tgcaatgtaa gcctggaccg caggcccttc ttcgggagac tgtgggtcct cgagcccaag
    22801 aagtctctga aaacgttcca ccgaatcgaa gagcacgtct aggagctgct gcgccacggg
    22861 agtccggccg agacgcaaat catccagcaa gtcctcgagc gcatgcgcga cggcgccaac
    22921 gtgcttgtac ccgaacatcc cggcgatgcc cttgagcgtg tgcacgcttc tgaagatctc
    22981 gttgatcagg gcaggattga tacgttcacc acgatggtgc gcctcgtcga gcagcaagag
    23041 gtctcgcgac aatgcttcga cgagctcctg agcctctgcc aggaactctt gttgaacgtt
    23101 gtcgcgggga tcgctcaccc ggctccccct gcagcggtgg acaacaaacc tcggatcaac
    23161 tctcgaagct catccacctc gaacggtttc tcgaggaagg cgtccgctcc aagctccgtg
    23221 ccccggcggg catcccgccc ttcgacctgg gtactgatta aaatcttccg ggccccttgc
    23281 tgccgagggc tcttcttcat gaaggccaag agctcgagcc cgttgatgtc gggcatgttg
    23341 acgtcgacga tgaccacatc gaacgcattg gacgccagca gccgtagcgc ctcgaagccg
    23401 gtcgcggcct cttcgacccg ggcgaaatcc gcatcctcca atgaggcgcg aacaaatgcg
    23461 cgcacggcgc tgctgtcgtc gacgattaga gcgtgaagaa ccactcgtac cccccgtgta
    23521 cagggtaaca gagacaaagt aggggggacc cgccctattc acgccaaaac gaacgcgatt
    23581 tcggagcatc cggcgcgcag gatgaacatg cgcacaggct tcgcaggaag cgatgactgt
    23641 agattcccgt ctggtgacca tcgcgccaca ccagtcgaat ggcgtaattg ccgacgggtt
    23701 gaacgtcgct gagctcgaag tcaccccctt cgacgaattg gatgggtccc tcgtgtccct
    23761 ggcagtgcgc gcatgggcag aagcccctca agagctcatg cggatacaca ctctgatgcc
    23821 cgtcggccca gtcgatctcc atcactcgtg ctccgcgagg cgcgcgaagc tccaccggct
    23881 cgggtgcgtc actcacgggg ttctgccttc ccagctgccc gacgccattg ccgtgcgctc
    23941 gcgaaccgtc acggtcgcat ggattgcatc aagctctccg agcgcgtcgt cgagctcttg
    24001 ccgctcgcgt tcgggccaag gctgctcggg aagctcgttt gtcgactgaa tcaggaccca
    24061 gcgccgtaga gcgccagccg agagaatcaa acggcctcct ccgcacgccc ggcatacgat
    24121 cccaccccgc gccgcatcga aggaggccgc gcggccgacg gcaggaacct taccgcaatg
    24181 aacacagcgg tcgagcgtcg gcgcaaatcc gagaatcgtg aggaccctga tctgaaggca
    24241 cagtagagcc tccttcgccg gcgctctttc gttcagagct gcgagaaagc gtaccgctgc
    24301 atcgaagacg gcaggctcgg gctcgtggtc gggtacgcgc tcccgaatga ccgccatcgc
    24361 agcacctgcg gatccaatcg cctccagact ccgaaggatc tcgtgaaacg atcggtcgac
    24421 ggaaacctgc tccaagtgtg cgagctcacc gcggccggga cggaagtgcg cgttcatcaa
    24481 cacgtagggt tgcagtgcgc cgccgaagcg ccgctggctc tttctagcgc cccgcgcgag
    24541 cgcagaaatc ttgccgtagt cctcggtcag gagagtgacg attcggtccg agtcgcgata
    24601 gtcgatggat cgcaggagga gcgctcggct ctggcggctt cgacttggca cggcagggca
    24661 gaggatagct caatcggatc cttctgtgca ggcgcccgcc tgaccaggac gaaagtcgcc
    24721 acgatgatcg caaaaagcgc cgctgtcacg acggcaagta ccaccgctcc atagcctctg
    24781 cggctatcga ccgtcgcaag gggagccggg ggcaaggtct tcggccgctg tcggtcgaat
    24841 ctctcgagaa cctcgtcagc atcgatgtga acggccgatg cgtaagcctt gatgaagcca
    24901 cgtacgaaaa cgccgctagg cagatcttcg aaacgatcgt gttcgagtga ttcgagcgtc
    24961 tttcggggaa tccgcgtcgt gctcgaaacc tcttccagcg acaaccgacg cgtttcgcgc
    25021 tcggtcttca agtagtcacc aatcgattcg cgcatcatcg cgagtcctcg agaatcgttt
    25081 ggcacatgcg accatcgttg gaataggggt cgagctccac acagcgctcg aggtctgcga
    25141 ctgcatcttg ccgatgacca agtcgcgcgc gcacctcgcc gcgtagacgc caagcgcctt
    25201 gaagctgggg gctcttggca caagaaggat cagcctgaag ggcgctcacg agagctcgct
    25261 cggcatcctt gagctgttgc aagtgccaaa gtgcttgcgc catcgtgaag tagcccacgc
    25321 agaacctcgg ctgtatccgg accgcttcca tcgtcgaggt caccgcttcc tgatattccc
    25381 ccgcgttcaa ttgtgccagt ccgaggttgc cccatgcaag gtgtggtgct gtgttcagtt
    25441 catcctcggc ggactgccga agcacgacga tcgcatcgtc gggacgatcc aactccatca
    25501 gacagagccc gtagatgttg cgcgcttcag cgagcgtgga gccgccccgc ttctgcttct
    25561 cgagaagctt gatcccagca ttcagatgct gctccgcatt ttcatagtcc cggcgctcca
    25621 tctggatgaa cccgagcagc agatgggcct cggcattgct ggaatcgagc tccaacgcca
    25681 tgcgcagatg ctcgatggca ctggccgtct gtccttcatc ccgaaggctt gctgcgagct
    25741 ggaactctcg aaacgagcgc tcgccgctcg gagttcccga cgcggaagtc gtcgcacatg
    25801 ccagggccag gcaagccagg caaactagtc cccaaattcg aagcacttgg aaggtctact
    25861 gagtggcgcg accggggtca agaaggggtg tcggattacc gacagctcaa tccgtggcgc
    25921 acatcctctt tgagcgcgag caaatcgtgc agttgccgca acgccgaggg gtcggagatg
    25981 gtgatcgtct gatcacctag gctgaggtat ccacggtctt ttagctgacc caccaccgcc
    26041 ttgacttgat cgagctccaa tgcagttcgg gtggataatt cgagaggaga aagctcgagc
    26101 ggcccggcgg tgtcgctctc ggctgcgcga aggaggctgt tcagcacccg gatcgtaggg
    26161 tcgggcagca agaagttctc gatctgctcc tctgctctct gcagacgccg ggcgagttgc
    26221 tgcatcacac tatcgcccac atcgggccgc ctgcgggcca acgcccggaa cgtatcgctc
    26281 tcgatcacca aggcggtcac aggttcgatc gcccgggccg tcgcattgcg atgggccccg
    26341 ggcaggaggg cttcctcgcc gacgatctcc tgcgagccaa aaagcccgac gctccgctcg
    26401 accccgcgcg cccgcttgat cagctgcacc cggccctcgc ggatcaagta gagctcggat
    26461 gccggcgcgc ccgcgtagta gagcgtggac ccagccgaga agctctgata atagcgctcg
    26521 atctcgagct catcggccga ttcgggcatc ggacctagtt accccagttg gcgtaaatcc
    26581 cgcaaccgct atcgggcgag atggcaggcc gcggcatgcc cctcagcaag gctccgaagc
    26641 gccggaacct ctcgatcgca aagacctttt tcggaaatgg gacagcgcgg gtgaaatgcg
    26701 cagccggacg ggggatcgag cgggctcggc acgtcgccct cgagcaagag ccgtcgcttg
    26761 cgcgcacctg ggtcaggttc gggcgcggcg ctgagcagcg cctcggtata ggggtgacgg
    26821 gggctggagt agatctgctc ggagcgggca agttccacga tccgaccgag gtacatcacc
    26881 gcaacgcgtc gactcacgtg ctccaccacg tgcagatcgt gtgcaatgaa gagaaaactg
    26941 agcccaagct cgacctggag gtcgctgagc aggttgacga tctgggcctg aatcgaaacg
    27001 tccagggcgc taaccggctc atccgccacg atgaagctcg gcgaaacggc gagcgcgcgc
    27061 gcgatgccaa ttcgctgacg ttggccccct gagaactcgt gtgggtatcg gcgcatctgc
    27121 tcggggcgca aaccgacgcg ttcgagcaat gccgcgatcc gttgctcctc ttccaacccc
    27181 gagcggacga tcccatggac tctcagcgct tctgcgagag cggcccgtac ggtcatcctt
    27241 ggattcagcg agctgtatgg atcttgaaag atgatctgca tctttctgcg gaagggtcgc
    27301 aacgctgact gcgacagctc ggtgatgtct tggccttcga aatagattcg tcccgacgtc
    27361 ggctcataga gccgaagaag cagacgtccc agggtgctct tgccgcaccc cgactcgcca
    27421 actaggccca acgtctcccc tcgcatcact tccaggctca cgccatcgac cgcgcgaagc
    27481 tcggcatgcc cacgcccgat gccgcgggac ggaccacgaa agtacttccg aagtgactct
    27541 gcacgtacga tcgtttgctc gctcacgcag agacctccgc atgaaagcag gcgacctcgc
    27601 tctgctcgag ctgctgcaag cccggttctt cgctgcggca tacctcgctc acgaggtcgc
    27661 agcggtcttg aaaccgacaa cccctcggca gattgcccaa gtctggcacg atgccacgaa
    27721 tggtcggcaa ccgctggcca cgattgcgag cgctgggcaa actccgaagc aaacctcgtg
    27781 tgtacgggtg tcgcggattt cggaacagtt cgttggtgct tgcgcgctcc acgatccgcc
    27841 ccgcgtacat gaccgcaata tcctgggcga attctgccat cacgccgagg tcatgactga
    27901 tgaagatgat gctcatccct agccgctgct gcagcgaccg aagcaggtcc aggatctgag
    27961 cctgaatcgt gacgtcgagc gcggtggtag gctcgtccgc gatcaacaga cgcggctcac
    28021 aagcaagcgc catcgcgatc atcgcgcgtt gacgcatacc tcccgaaagc gtgtgcggat
    28081 aggagtcgac ccgctcggcc ggagcgggga tcccgaccaa gtccaagagc tcgatcgcgc
    28141 gcgccttcgc ttcgcgccga gtcatctttc gatgcaggcg aatcgcttcg acgatctgcg
    28201 agccaaccgt gtagacggga ttgagcgagg tcataggctc ttggaaaatc atcgaaatct
    28261 cgttgcctcg gagcctgcgc atctcggact cggatatctg catgagatct cgtccgtcga
    28321 acagagcttc gcctccttcg atgaagccag gcggactcgg aatcaagcgc atgatcgaca
    28381 aggacgtcac gctctttccg caaccgctct cccctacgat accgagcgtg cggccttcga
    28441 aaacatcgaa cgagacgtca tcgacggctc tcaaatagcc ttcgtcggtc tcgaacgacg
    28501 tgatcagatg gcgcagactg agcagcggtt ctgacatgcg gcgctacctt agcaccaccg
    28561 agccgatgct cggcttttca tgagcaaggg gcgaattcaa tcgacatgcc cggccctgga
    28621 gcctgctgaa atgcgtctgc cggaacctcc gggtcgacga atatcgattt cacgcgaacc
    28681 gacgtgtccg cgtcgttgac ctcgtcggtg aaccgcacat tcgtcggaaa cgaatgaccg
    28741 tcgatctcga tgtgttcgtc gtaggtcgct gtccagcgcg tcttgccgtc aggacctcgc
    28801 tccgtggagc ggcggagcgt caatcgttgc tccccgggcg cctttgcacg atccgactcg
    28861 tctatggaaa acgctagctc ttgcgtagac ccatccgcgg cgacgagggt gacgaggtag
    28921 cgcccgtccc ggcattccat cgactgctcc tccgcttcga tcaggggcgt gtcgcccgtg
    28981 agaagcagaa gaacgttctc ggcttcgacc gagattccga gcagtcgcgc gatgttctgc
    29041 ggacaagtcg ggccatggag aaaagtcccc gcgcggagat cgctcagttg aaagctctcg
    29101 ccatcgctcg ttagaaccgc agcaggtccg accggcgtca tcacatcgaa tcgtacccgg
    29161 cttgggcgtt cgagaaacat tagcaccgta ccgcgaacgc gaccgttgcg gccccactgc
    29221 gtcaccctgg cttcggcctt gatcgatctc caccctgctt gccgctgcgc atgatcttcg
    29281 agcgcctttg ccgcgtcggt cctcgcctga acaggacatg cggggacgct cttgcatcct
    29341 gcgatggaga ggacggaaca gagcaatgct gtgaggcagg cgcgtgtcac tagtcgttgg
    29401 gcgcgccgag cgcgatccac tcttcgacga cttgaatgag gctttcgctg atcgagccgc
    29461 cgagcggcat ccgatcgccg caggtctgaa cgccccgcat cttctgaatc attatcgagc
    29521 cgggagaatc gtccggtatg acgagcgtgc cctgccccgc gcatgcgcca gggtcagctg
    29581 ccaatgcgtc gaccactgct gaccaggatt cgctgggcca actcgcggtg ttcgtcgtat
    29641 ccgcgccggt gaggttcaat gccgccagac ccgcggggct tgcgccccca ggaatcgcgc
    29701 cgcctatggg attgtcgggg ttctggtcga cgttgctgtt ggccgggccg tggcagatca
    29761 cacagctctg gaacatgatg ctccagtaga tatcgctcca ggctggatcg ggcagatctc
    29821 cctcggggcc gttgtaaatg cagatttcgt cgtcgaccga agcacaggcc gtgagctcga
    29881 gctccgcgct cggtggaatc tccgttcgtg caacgaccgg cgcttcacat gcgagccaat
    29941 tgcggatgat ctcctcggct tctttggttc cgatcgaagg tagctccgag ccatcggatt
    30001 tccgaatcca tggcgtgttg tttcgaacgc tttcacccgc agcgccgggc ggcatcgacc
    30061 cggcgcggat ctcttgaatc atcccttccg cccagtcatt gaccgtgttc acattgctct
    30121 gcagccgcga gagacggcta caatacggcg tgctggtttg accgctttcg caactggcga
    30181 tcggttgaga acaagagggg tcaagcgaat cgtcgatgca ggccaggttc acgtcgaaat
    30241 tcaaacctgc cggaacgcca atcctttcgg taccggtcgc agcgggcgaa tggcagaacg
    30301 ctccgtcgcc gcaggtcgac tgcacgagcg cttgcccttc gtacatcggc aagccgtcgg
    30361 tgaggcgata cgcgatgtcg aacgc
//
LOCUS       DASRZF010000166         3936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1486178, whole genome shotgun sequence.
ACCESSION   DASRZF010000166 DASRZF010000000
VERSION     DASRZF010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1486178"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2040)
                     /locus_tag="VFG22_07635"
     CDS             complement(<1..2040)
                     /locus_tag="VFG22_07635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HET6416153.1"
                     /translation="MPEIKVKLTSEGYAKVAREVDKVDQKTEKATKSSAGYTKALIAI
                     GAATVGIVAIRRSLEDVIRTGAAFQDSMTKSLAIMGDLTSTLENDLTNAARKAGKESV
                     FSANQAADAYFFLASAGLSAEQSIAALPIVLATATAGAFDLATATDLLTDAQSALGLS
                     SKDAAENMENMARVADVLVKANTLANATVRQFSEALTTKAGTALRIVNKSVEEGTAVL
                     AAFADQGIKGALAGDALNIVLRDLSNAALSAPKAFERLGIEVFDSAGKMRHMADILAD
                     LEGALEGASDEQTRITLKQLKFQDRSVSYLLALVGMSDKIRDMDASLQAAGGTVEKIS
                     EKQMRSLVNQTKLVTGRIEDLKIEIFQQLEPALAAAVKSTKEWLDANDEMIKADIIGF
                     VGNAADAWRDMGDVLGAVKDDFRDIVGLVKDIPEAFWQLGAPLLLFAAGRPVLGTLAV
                     GAQVVRPVARSFTASRDEAFARAEIERQEKFTGGALSSEEINRILAGAHAARRQAEDN
                     AASESGGNQRFAAGGALQSQILKALDTKDSGGLNTLKAFSGDLGLVEDAFGNVVGAMS
                     LAEPVFQNASAAFGEITKTAGAASKVVKGAGDSAEMSAAQWTKNAGALFLVEQKISDN
                     QKALDDWRESQIRAIDSMIAMGAEANAIRLNVRPYQALGDEIERIERLATMFPDLL"
     gene            complement(2033..2287)
                     /locus_tag="VFG22_07640"
     CDS             complement(2033..2287)
                     /locus_tag="VFG22_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416154.1"
                     /translation="MKATLDQPSLGFPEAYYWDRYCQLSAYRSGELQPQPISIPDIAA
                     YCDLLGITAPSIRENMLDAVTILEAESRRLRTEDNEASNA"
     gene            complement(2365..2793)
                     /locus_tag="VFG22_07645"
     CDS             complement(2365..2793)
                     /locus_tag="VFG22_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416155.1"
                     /translation="MTKAKAKGTNLFKLLETDPDLEAEGVWCAYPGTQSRFKVRSLEG
                     SDAEKYRQKLIQERVEEIREGGESQRVALTDIAQSVLSDVLVADWDGVLGDDDEPLPF
                     SIENCRLLLARVRPVRSWLERQCASTETYLLRKDVVGSGN"
     gene            complement(2825..>3936)
                     /locus_tag="VFG22_07650"
     CDS             complement(2825..>3936)
                     /locus_tag="VFG22_07650"
                     /inference="COORDINATES: protein motif:HMM:NF039819.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HET6416156.1"
                     /translation="QSAELRSDRQIPYLAEVGAGASGGINMELSYGTYDSWLESLLSN
                     VWVPLTGGARQQLGSTVITNPAGTIAATGIHLGVVVGQYIRLDTCEDPANDGFYLVTT
                     VGTDTITVTPSPPTTNIADTTVFVSADHLENGVTEKSFSIEREFAFGVSPAVTKRFTM
                     FNGMEVQALSLNLTEGAIIGGGFTFLGKEGTVATATGAGTPVAATTTDPINATSNVGT
                     ISDTYGLATRATLSTALQSLTFNIDGSLRQRRAIANRTACGIGAGRFQVTGSVNAYFE
                     DGALFEKYILHTATSLSANIHDGATGASAAATLANRIAGNTYIITFNRVQFAQSPGPS
                     AGATDADVFTNLNFQGIYDSETGCTVQIDRFPAIA"
BASE COUNT          700 a         1240 c         1119 g          877 t
ORIGIN      
        1 caataggtcg gggaacatcg tggcgagtcg ttcgatgcgt tcgatctcgt ccccgagagc
       61 ttgatacggg cggacattga gccggatcgc attggcttcc gcgcccatag cgatcataga
      121 atcaatcgcc ctgatctgcg attctcgcca atcgtccaac gccttctgat tgtcactgat
      181 cttctgctcc acgaggaaca gcgccccggc attcttcgtc cactgtgccg cagacatttc
      241 ggcggaatcc ccagcgccct ttactacttt cgatgctgcg cctgcggtct tcgtgatttc
      301 accgaatgcc gcgcttgcat tctggaatac cggctctgcc aatgacattg cgccgactac
      361 attcccgaac gcatcctcga cgagtccgag atccccgctg aaggctttga gcgtattgag
      421 ccccccggaa tcttttgtat cgagcgcctt gaggatctga gattgcagcg cacccccagc
      481 agcaaagcgt tgattcccgc ccgactcaga tgcagcattg tcttcggcct gccgtcgtgc
      541 agcatgtgcc ccagcaagga ttctgttgat ctcctcgctt gagagagcac cgccggtgaa
      601 cttctcctgc cgctctatct ctgcacgggc gaatgcttca tccctggaag ccgtgaaact
      661 tcgcgccaca ggtctaacca cttgcgcccc tacagcaagt gtgccgagta ccggtcgccc
      721 tgccgcgaat aggagcaacg gcgctccgag ttgccagaag gcttcgggga tgtccttgac
      781 gaggccaaca atatctcgaa aatcatcctt cactgcgccg agaacgtcgc ccatgtcgcg
      841 ccacgcatcg gcagcgttac cgacgaagcc gatgatgtcg gcctttatca tttcgtcgtt
      901 cgcgtcgagc cattccttcg tggatttcac agcggcagca agcgccggtt cgagctgctg
      961 gaaaatctct atcttgaggt cttctattct gccggtgacg agcttcgttt ggttcaccag
     1021 cgaccgcatc tgcttctcgg aaatcttctc tacggtcccg cctgcggctt gcagcgatgc
     1081 gtccatgtcg cggatcttgt cgctcatacc gacaagcgcg agcaggtacg acacggagcg
     1141 atcctggaac ttcaactgct ttagggtaat ccgcgtctgc tcgtccgacg cgccctctaa
     1201 tgccccctcc aggtccgcga ggatgtcggc catgtgccgc atcttcccag ccgaatcgaa
     1261 tacctcgatt ccgaggcgct cgaatgcctt cggtgcgctc agtgccgcgt tcgaaaggtc
     1321 gcggaggacg atgttgagag cgtccccggc gagagcaccc ttgatgcctt gatcggcgaa
     1381 tgctgccagt accgccgtgc cctcctcaac gctcttgttc acgatacgga gcgcggtccc
     1441 cgccttcgtc gtcagcgctt ccgagaactg cctcaccgtt gcgttggcta gcgtgttggc
     1501 cttcaccaat acgtcggcga cgcgggccat gttctccatg ttctcggctg cgtctttcga
     1561 cgataaaccg agggcggatt gcgcatccgt caggaggtcc gtagccgtcg ctagatcgaa
     1621 tgcaccagcc gtcgccgtcg cgagcacgat gggcaacgcg gcgattgact gctccgccga
     1681 gagtccagcc gaggccaaga agaagtaggc gtctgcggcc tggttcgcgg agaagacgga
     1741 ctccttgccc gccttccgcg ctgcgttcgt gagatcgttc tcaagcgtcg aggtcaggtc
     1801 gcccatgatg gcgagcgact tcgtcatcga atcttggaag gccgcgcccg ttcggattac
     1861 gtcttccaac gagcggcgga ttgcgacgat tccaaccgtc gctgcgccga tagcgatcag
     1921 cgccttggtg tatcccgcag acgactttgt cgccttctct gttttctggt ctaccttgtc
     1981 gacttcacgc gcaaccttcg catagccttc ggaggtaagt ttgactttga tctcaggcat
     2041 tgctcgcctc gttatcctcc gttcgcaggc ggcgcgactc ggcctccagg atcgtcaccg
     2101 cgtcgagcat gttctcgcga atggacggag ccgtaatccc taacaagtcg caatacgcgg
     2161 cgatgtctgg gattgaaatc ggttgcggct gaagctcgcc gctccggtaa gcggacaact
     2221 ggcaataccg atcccagtag tacgcttcag ggaacccgag cgaaggctga tcgagtgttg
     2281 ccttcaccgt aatcccctcc ttccttagcc gttcaaacca tccggctttt gcggcccaac
     2341 gccagctcca gcgcagggcc gcaatcagtt tcccgagcct accacatcct tccgaagcag
     2401 gtatgtttcc gttgacgcgc attgacgctc tagccaggag cggaccggcc tgacccgagc
     2461 gagcagtagg cgacagttct cgatggaaaa cggcaacggc tcgtcgtcgt ccccgagaac
     2521 tccgtcccaa tcggctacga gtacatcgga tagaacggac tgtgcgatgt ccgtcagtgc
     2581 aacccgctgc gactctccgc cttcgcggat ttcttccact cgttcctgga tcagcttttg
     2641 ccgatacttc tcggcgtctg acccttccag ggatcggacc ttgaaccggc tttgcgttcc
     2701 agggtaagcg caccacactc cctccgcctc taagtccggg tctgtttcca gcagcttgaa
     2761 aaggttcgtt ccctttgctt ttgctttcgt catctcggct cctgtctgcg tgctgcgcta
     2821 ccgcttatgc gatggcgggg aatcggtcga tctgaacggt gcagcctgtc tctgaatcgt
     2881 agatgccctg gaagttcagg ttggtgaaca cgtcggcatc agtcgccccg gcactcggtc
     2941 ccggtgactg cgcgaattgc acccggttga acgtgatgat gtacgtgttc ccggcgatgc
     3001 gattcgccag cgttgcggct gcgctcgcgc cagttgcccc gtcgtggatg ttggccgaga
     3061 gcgaggtcgc ggtgtgcagg atatacttct cgaagagcgc accatcctcg aagtaggcgt
     3121 tgacgctgcc ggtgacttgg aagcggcctg cgccgatgcc gcacgcggtc cggttcgcta
     3181 tcgctcggcg ctgccgcagc gacccgtcga tgttgaacgt gagactctgc aacgcagtcg
     3241 agagcgtggc gcgagtcgcc agcccgtagg tgtcgctgat cgtacccaca ttgctcgtcg
     3301 cgttgatcgg gtccgttgtc gttgcagcca ccggcgtccc tgcgcccgtt gccgttgcta
     3361 ccgtgccctc cttgccgaga aaggtgaagc cgccgccgat gatcgcgccc tcggtgagat
     3421 tcagcgacaa cgcttgaacc tccatgccgt tgaacatcgt aaagcgcttg gttacggctg
     3481 gcgagacacc gaatgcgaac tcccgctcga tcgagaacga cttctccgtg acgccatttt
     3541 cgagatggtc tgcgctgacg aagaccgtcg tatcagctat gtttgtcgtg ggcggagacg
     3601 gagtcacggt gatcgtgtcc gtgccgacgg tagtgacgag gtagaatccg tcatttgcgg
     3661 ggtcctcgca cgtatcgagg cggatatact gcccgaccac aacgccgaga tggatacccg
     3721 tcgcagcgat agtcccagcc ggattcgtga tgacggtgct gccgagttgc tgccgagcac
     3781 cgcctgtcag cggaacccat acgttcgaca acaaggactc cagccatgag tcgtaggtgc
     3841 cgtaggacag ctccatgttg atgccgccgc ttgcacctgc gccgacctct gcgagatacg
     3901 ggatctgtcg gtcactgcga agctctgcgg actgaa
//
LOCUS       DASRZF010000167         2031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1832450, whole genome shotgun sequence.
ACCESSION   DASRZF010000167 DASRZF010000000
VERSION     DASRZF010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1832450"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VFG22_07655"
     CDS             <1..284
                     /locus_tag="VFG22_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416157.1"
                     /translation="VEGHVFSKLTGVPITSASVEVSAVGSNHEPWLIVGRALSDGNGF
                     YSIRINAEDVDRAHLSIHASCLTEAGLKTTNRWTSPVDLRPNPQRRDLY"
     gene            332..1563
                     /locus_tag="VFG22_07660"
     CDS             join(332..614,614..1563)
                     /locus_tag="VFG22_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611958.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HET6416158.1"
                     /translation="MARRNKFSQEVRERAVRMVSDHRADYGSEWEAIRSIAEKIGCSP
                     EALRTWVRRVQVDSGSRAGVTSDERARMKDLEKENRELRRANEILRKASALFRTGGAR
                     PPTQMMVEFIDDYREEYGVEPICRVLPIAPSTYHEQKARQADPSRLPERWVRDAELRL
                     EIERIWKENRSVYGARKTWLQMKREGFSVARCTVVRLMRHMELRGVVRGKQYKTTTIV
                     DEEAQRPVDLVQRNFTAERPNQLWVADITYVATWAGFVYVAFVTDVFSRRIVGWRVSR
                     SLRSDLALDALEQALHARPEKEKLIHHSDRGVQYLSICYTERLAEAGIEPSVGSVGDS
                     YDNALAETIFGLFKAEVIWPNGPWRSFEEVEFATLEWVDWFNNQRLLEPIGDIPPAEF
                     EAMYYARHESPPTEVGLN"
     misc_feature    610..726
                     /locus_tag="VFG22_07660"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     gene            complement(1667..>2031)
                     /locus_tag="VFG22_07665"
     CDS             complement(1667..>2031)
                     /locus_tag="VFG22_07665"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET6416159.1"
                     /translation="QTAPSVGPGVAKALIVDLPELGALGRRQITSLVGLAAFAKDSGK
                     KSGYRRIRAGRAAPRTALYLAAMNGARFNPVLKPMYERMIAAGKPPKVAFIALARQLV
                     TILNAMIRDRNDWQQVTN"
BASE COUNT          410 a          608 c          630 g          383 t
ORIGIN      
        1 ttgtcgaagg ccatgtattc agtaagctca cgggcgtccc catcacaagt gcctcagttg
       61 aggtctcagc ggtgggaagt aatcacgaac cctggctaat cgttgggcgc gcactatcgg
      121 acggaaacgg tttctattcc atccgcatca acgcggagga cgtcgaccga gcccacctca
      181 gcatccacgc cagttgcctg accgaagctg ggctcaagac aacgaaccgc tggacgtcac
      241 cggtggacct tcgacccaat ccccaacgcc gggacctcta ctgaaccgcc ccgggtttgc
      301 cggaggctcc atttcttgag aggatggagc catggcacga agaaacaagt tctcacagga
      361 ggtgcgtgag cgagcggtcc ggatggtttc ggatcatcga gccgactacg gctcagaatg
      421 ggaagcgatc cgctcgatcg ccgagaagat cggctgttcg cctgaggccc tccgcacctg
      481 ggtgcggagg gtgcaggttg attccggttc tcgagccggt gtcacaagcg atgaacgggc
      541 tcggatgaag gatctcgaga aagagaatcg agaacttcgc cgagcgaacg agattcttcg
      601 caaggcgtcc gcctatttcg cacaggcgga gctcgaccgc cgacccagat gatggtggag
      661 ttcatcgacg actaccgaga ggagtacggg gtcgagccga tctgcagagt cctgccgatc
      721 gccccgtcga cctatcacga acagaaggcc agacaagctg atcccagtcg gctgcctgaa
      781 cgatgggttc gcgatgcaga gctgcgcctc gagatcgagc ggatctggaa ggagaaccgt
      841 tcggtctacg gagcccgtaa gacctggctg cagatgaagc gagaaggctt cagtgtggct
      901 cggtgcactg tggtccggct gatgcggcat atggagcttc gtggtgttgt tcgcggcaag
      961 cagtacaaga cgaccacgat cgtcgatgaa gaagcgcagc ggccggtgga tctcgtccag
     1021 cgcaacttca cggccgaacg acccaaccag ctttgggtcg ccgacatcac gtacgtcgcg
     1081 acctgggcgg gcttcgtcta tgtcgcgttc gtgaccgatg tcttctcgcg ccggatcgtg
     1141 ggctggcgcg tatcgaggtc gctacgaagc gatctcgctc tcgatgcttt ggagcaggcg
     1201 cttcacgcac gacccgagaa agagaagctg atccatcaca gcgaccgcgg cgttcagtat
     1261 ctctcgatct gctacacgga gagattggcc gaggcgggca tcgaaccctc ggtgggcagc
     1321 gtcggcgact cgtatgacaa cgccctggcc gagacgatct ttggcctctt caaagccgaa
     1381 gtgatctggc ccaatggccc ctggcgaagc ttcgaggagg ttgagttcgc aacgctcgaa
     1441 tgggtcgact ggttcaacaa ccaacgcctt ctcgaaccga tcggggacat acccccggcg
     1501 gagttcgagg cgatgtacta cgcaaggcat gaaagcccgc ccacggaggt cggactcaac
     1561 taaacgagcc tccggagaag ccggggcggt tcactgggcg ggtctctcgc acgctgtcga
     1621 gctccggagt ccgccgtcta acaagcaacc ctgattcagt ttctttctag ttcgtaacct
     1681 gctgccagtc attccgatct cgaatcatcg cattgaggat ggtcacaagc tgcctggcga
     1741 gggcgatgaa ggcgaccttg ggcggttttc ccgcagcgat cattcgttcg tacattggct
     1801 tcagcaccgg gttgaagcgc gccccgttca tcgccgcgag gtacaacgcg gtgcgaggtg
     1861 ctgcgcgccc cgcgcggatc ctgcggtatc cgctcttttt cccgctgtcc ttcgcgaacg
     1921 ccgccagacc cacgagcgat gtgatctgtc gccgccccag agcgccgagc tcgggcaggt
     1981 cgacgatcag ggcttttgca actccaggcc ccaccgaggg tgccgtctgc a
//
LOCUS       DASRZF010000168         6734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3897037, whole genome shotgun sequence.
ACCESSION   DASRZF010000168 DASRZF010000000
VERSION     DASRZF010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 6734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6734
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3897037"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1822)
                     /locus_tag="VFG22_07670"
     CDS             complement(<1..1822)
                     /locus_tag="VFG22_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416160.1"
                     /translation="MSTSRKDSWAPRATRWVIPALLLLCAFGFARFYAQFYPIGEWLF
                     WRYASYWVACLFWSAGCLSAGYALVRRLRGTPLPFAETLCVSFAAGVVLFYLAINALG
                     SINGLHASVFFALPLVMIAIGAGPLWRLCRRYARHVRYRSSGRRRPSVLHLAIWAFGI
                     AVLAMIYFQMLTPENVQFDARWKHLALAEQYAHRGFIPRFGEGWTVATNPHLASILFT
                     WAFLLPGGVLFDQVELAAHMELTCFIWTLATIPPAVRLLVPGARARASWVARLLFPGV
                     LLYDSSLAAGADHIAALFALPIFILMLRSIPGLQPGRLALLALLMAGAAMPKLTAGMM
                     LVPGPALVIASVFIWRAARERRLSWTGPAAAVAVALGATAFFWLRNWIWYGDPLYPSL
                     HEYLTLRPWSQDAATLFEFGYKEYQFWRPSRDWEGVKETLSALATFSFIPHDYGRYHG
                     AVPVFGSLYTLLLVALPFLRRTRRIWLLVILTQLAIFTWFWVHHQDRYLQTLMPWMAA
                     VTAALMIRIWRTGWGSKLALSALVATQVLVGSDVYFVQTHAMIRSPIKKVNDLLSAGY
                     EKRYEQRLDVFKSWTRARDAIPEDAHVLLHDNHVHLGLSRA"
     gene            1985..2914
                     /locus_tag="VFG22_07675"
     CDS             1985..2914
                     /locus_tag="VFG22_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416161.1"
                     /translation="MIKQTIARILILASTLLVLSSCKKDSTTAADASQDPVLLLDAGA
                     EPKAPLRYKVAEGTTTKSNMDFTIATLATTDDAAALSIVPGVRLHIVSGPAMLTDKGV
                     KFEVNITKAEAAVPQGLDPDLAAELQQSAEILDDVGGTVEMNDRGIALSAELNDKAKS
                     PELPVRLLMMIVNARSTLSRVVLPAEPVGLGARWESRKELTLYGFKVQQVDSYTLVER
                     VGDEIKLSVVVTQNALPQTVDFPDEEISIGVEQMRANANGEIILNLNALESDAAASGE
                     ATDKLSVTGPEGSKKVELNEAFEIRMTNTTAYE"
     gene            2993..4447
                     /locus_tag="VFG22_07680"
     CDS             2993..4447
                     /locus_tag="VFG22_07680"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET6416162.1"
                     /translation="MSEHRETFDAVVVGSGPNGLSAALALAQAGASVLVLEAADEIGG
                     GTRTAELTLPGFQHDVCSAVHTTGILSPFFRSLPLEQHGLEWILPRASVAHPLDDQPA
                     VIMWRSVEETCAGLGADASSYRDLVSPFLGNPEGFFADALGPLGWPEHPLSFTRFGLR
                     ALWPATTFATWRFQQSRARALFAGCAGHSILPLSKMFTAALGLIFSIAAHVEPWPVAA
                     GGSAAIPRALGNLLRQLGGQIRTGVRVTTAADLPDARVYLFDTSPDQLASIAEPVLPG
                     GYLRRLRRYRYGPGAFKLDWALDGPIPWRDPSCLEASTVHLGGTLEEIADGEAKVFRG
                     EHPSRPYVLLCQQSQLDASRAPEGKHTGYAYCHVPGGSTVDMTEAIENQVERFAPGFK
                     ERILARHSMNTRDFQEYNPNFVGGAVTGGVADIFQLFTRPVARLDPYTTPNPRLFICS
                     ASTPPGGGVHGMCGYHAAQSALKRIERFEPESLV"
     gene            complement(4453..4824)
                     /locus_tag="VFG22_07685"
     CDS             complement(4453..4824)
                     /locus_tag="VFG22_07685"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET6416163.1"
                     /translation="MPPLELAALYLLAFAMVAIGILHFVRPKPFVRIVPKYLPAPLAL
                     VYISGFFEILGGLGLLIPSTRSWAAWGLIALYIAVFPANIYMLTDNISLDPRKPIPRW
                     ALWLRLPFQLLFIAWAYWFTG"
     gene            4872..6119
                     /locus_tag="VFG22_07690"
     CDS             4872..6119
                     /locus_tag="VFG22_07690"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET6416164.1"
                     /translation="MGKERSKPLRAEQFSTARAAQPFWYRAINGVGRLLPRLGNPSAE
                     AWFAKSKRQNPGVGNPRPETLAALDALLRSLREDAALSFAGRIAAQMDCLRMAGQHLL
                     VEQALAEKPEILATEIPSPIFLIGWMRTGTTFVHRLLAQDPDTRTMPYWESMYPAPPK
                     GTKDERPVQLARLLEQLESISPNYAAIHPMGAEEPEECVALFTNVFRTLQYNVQYRVP
                     SYVSWLQGQDPRIAYGQYRQQLQLVHYHRPHGTRFALKDPTHSVFVETILELFPDARF
                     VFTHRDPATTLSSLCSLYAYTRALFSDDVHAEALGPELLNSYLPPTLEHALEVVDGLP
                     AGRVAHVRHVDVRRDPISAMASAYRDLGMELGEPARAAMQKMLDAESRKPRDIHIHSP
                     EAFGLSADEVRARLASYCARFDL"
     gene            complement(6114..6638)
                     /locus_tag="VFG22_07695"
     CDS             complement(6114..6638)
                     /locus_tag="VFG22_07695"
                     /inference="COORDINATES: protein motif:HMM:NF019150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1523 family protein"
                     /protein_id="HET6416165.1"
                     /translation="MRKLGIALAVLVTLLFAGTLVYFMPRATMARITGTDVKRMDKTD
                     MAGGEGTTRDVRFLYATELDSKKARVFRNEDNGWYLKWNSGDIAAEASKLASKEEEKI
                     ALVKFYGLRIAILDSYPNVLSLKEVDADYVYVPWVNMVVLLVLLVFVIWAGVKIRHAF
                     GSAKAKLSGKPRAS"
BASE COUNT         1316 a         2090 c         2146 g         1182 t
ORIGIN      
        1 tagcgcgaga taggccgagg tggacgtggt tgtcgtgcag caatacgtgt gcgtcttcgg
       61 gaatggcgtc acgcgccctc gtccacgatt tgaagacgtc gagacgctgc tcgtatcgct
      121 tctcatagcc ggcggaaagg aggtcgttca ccttcttgat gggggagcga atcatcgcgt
      181 gcgtctgcac gaaatacacg tcacttccga cgaggacttg tgtggcgacg agcgcgctga
      241 gggccaactt ggacccccaa ccggtgcgcc atattcgaat catgagcgcc gcagtgaccg
      301 cagccatcca aggcatcagc gtttgcaggt accgatcttg gtgatgaacc cagaaccagg
      361 tgaagattgc gagctgagtc aagatcacga gaagccagat gcgccgagtc cgccgaagaa
      421 aagggagggc tacgagcaga agcgtgtaaa gcgagccgaa tacgggaacg gcaccgtggt
      481 agcgaccgta gtcgtgcgga atgaatgaaa acgtcgcgag ggcgctgagc gtttctttga
      541 cgccttccca atcccggctg ggtctccaaa attgatattc cttgtagcca aactcgaata
      601 gcgtggcggc gtcttgagac cagggacgta gcgtcagata ctcatgcagc gacggataga
      661 gcgggtcccc gtaccaaatc cagttgcgta gccagaagaa cgccgttgcg ccgagggcga
      721 cagcgacggc cgcggccggt ccggtccaag agaggcgtct ttcgcgggcg gcccgccaga
      781 tgaacacgct tgcgatgacc agtgccgggc ctgggaccag catcataccg gcggtgagct
      841 tcggcatcgc cgcaccggcc atgagcaacg cgagcagcgc gaggcggccg ggctgcaagc
      901 cggggatcga gcggagcatc agaatgaaga tcggcagcgc gaagagcgcg gcgatatggt
      961 ctgcgccggc tgccagcgag gagtcgtaca gcagcacgcc gggaaagagg agccgtgcca
     1021 cccaggaggc ccttgcgcgg gcgcctggca cgagaaggcg cacggccggc gggatggtgg
     1081 ccagagtcca gatgaaacag gtgagctcca tgtgcgctgc cagctccacc tggtcgaaga
     1141 gcacgcctcc tggcagcagg aatgcccagg tgaagaggat gctcgccaaa tgcggattcg
     1201 tagcgaccgt ccaaccctct ccgaaccgcg ggatgaatcc tcggtgcgca tactgctcgg
     1261 cgagcgccag gtgcttccag cgggcatcga actgaacgtt ctcgggcgtg agcatttgga
     1321 aatagatcat cgcgagaact gcaatgccga atgcccagat ggcgaggtgc agaaccgacg
     1381 ggcgccgacg ccccgaagat cgataacgaa cgtggcgcgc gtatcgtcgg caaagtcgcc
     1441 atagcggacc cgcaccgatc gcgatcatca ccaatgggag ggcgaagaac accgatgcat
     1501 gaaggccatt gatgctgcct agggcattga tcgcgaggta gaagagcacc acacccgccg
     1561 caaagctgac gcagagcgtt tccgcgaacg gaaggggcgt cccgcgcagt cgacggacca
     1621 gcgcatagcc tgcgctcaag cagcccgcgc tccagaacag gcatgccacc cagtacgagg
     1681 cgtagcgcca aaacagccat tccccgatgg ggtagaactg cgcgtagaat cgggcgaatc
     1741 cgaacgcaca gaggagtagc agcgcaggaa tgacccagcg cgtggcccgc ggtgcccagc
     1801 tgtctttacg gctcgtgctc accgatttcc acctcttgaa tcgctcataa cacagtctcg
     1861 gcggtagggc ggctaaggac cgctcgctgc agccttggtc acggcgacca acggcttgac
     1921 catctcgagc ctcgcgacta agtgggccgc accggacggg attcgaacgc cggtaggaga
     1981 gccaatgatc aagcagacca tcgcacgtat tttgattctc gcatcaacgc tgctcgttct
     2041 cagcagctgt aagaaggact cgactaccgc cgccgacgcc agccaggacc cggtgctcct
     2101 tctcgacgcc ggcgccgagc cgaaggcacc gcttcgctac aaggtcgcgg agggtacgac
     2161 gaccaagtcc aacatggatt tcacgattgc gactctggcg acgaccgacg acgcggctgc
     2221 gctttccatc gtgccaggcg tgcggcttca catcgtttcc ggacccgcca tgctcaccga
     2281 caagggcgtg aagttcgagg tcaacatcac caaggctgag gccgccgtgc cgcaaggcct
     2341 cgaccccgat ctcgctgccg agcttcagca gagcgcagag atcctcgacg acgtgggtgg
     2401 cacggtcgag atgaacgacc gcggtatcgc tttgtccgcg gagctcaacg acaaggcgaa
     2461 gagccccgag ctccccgtgc gtcttctcat gatgatcgtc aacgcacgca gtacactttc
     2521 ccgcgtcgtc ttgccggcgg agccggtggg cctcggagcg cgatgggagt cgcggaagga
     2581 gctcacgctt tacggtttca aggtacagca ggtcgattcc tatacgctcg tcgagcgggt
     2641 tggcgacgag atcaagctca gcgtggtggt cacgcagaac gcgcttccgc agacggtgga
     2701 ctttccggat gaagagatct cgatcggcgt cgagcaaatg cgggccaacg cgaatgggga
     2761 gatcatcctc aacttgaatg ccttggaatc ggatgctgcg gcatcgggcg aggccaccga
     2821 caagctcagc gtcaccggcc ccgaaggatc caagaaggtc gagctcaacg aggccttcga
     2881 gatccggatg accaacacga cggcttacga atagcgctcg agcgctccga aaaaggcgcg
     2941 gtgcgaccct tttttgagat ggttacgctg cgagcgggac tatgttcttg ccgtgagcga
     3001 gcatcgggag acatttgacg ccgtcgtcgt cggctcgggg ccaaacggtc tgagcgctgc
     3061 gctggccttg gcgcaggctg gggcctcggt cctcgtgctc gaagctgcgg acgagatcgg
     3121 cggaggcacg cgcaccgccg agctgaccct accagggttt cagcacgatg tttgctcggc
     3181 cgtgcatacg accggcattc tctcaccgtt cttccgttcc ttgccgctcg agcagcacgg
     3241 gctcgagtgg atcctgccgc gggcctcggt cgcgcatccg ctggacgatc agcccgccgt
     3301 gatcatgtgg cgctcggtcg aggagacctg cgccggcctc ggagcggatg cgagcagcta
     3361 tcgagacttg gtttcgccct ttctcggcaa tcccgaaggc tttttcgcgg atgcgcttgg
     3421 gccgctcggc tggccggagc atccgctgag cttcacgcgt ttcggtctgc gggcgctctg
     3481 gccagctacc acgttcgcaa cctggcgctt ccagcagtcg cgcgccaggg cgcttttcgc
     3541 gggctgtgcg ggtcacagca ttctcccgct gagtaagatg tttaccgcgg cgctggggct
     3601 gattttctcg atcgcggctc acgtcgaacc ctggccggtc gctgccggag gctcagcggc
     3661 gattccccgc gcgctcggaa atctgttgcg tcagctcggt gggcagattc gcacgggcgt
     3721 tcgtgtcacc accgcggccg atctgcccga cgcccgggtc taccttttcg acacgagccc
     3781 cgaccaactc gcctcgatcg cggagcccgt tcttcctggc ggttacctgc ggcgacttcg
     3841 gcgctatcgc tacggccccg gtgccttcaa gctcgattgg gcgctcgacg gtccgatccc
     3901 ttggcgggat ccgagctgct tggaagcctc gacagtgcac cttggtggca ccctcgagga
     3961 aatcgccgac ggtgaggcca aggtgtttcg cggtgagcac ccaagccggc cctatgtgct
     4021 cctctgccaa cagagtcagc tcgatgcgag ccgcgctccg gagggcaagc acaccggcta
     4081 cgcgtactgc cacgttccgg gcgggtcgac cgtcgacatg accgaagcga tcgagaacca
     4141 ggtcgaacga ttcgcaccgg gcttcaagga gcgaatcctc gcccggcaca gcatgaacac
     4201 ccgcgacttc caggagtaca acccgaactt cgtaggcggt gcggtgaccg gcggtgttgc
     4261 ggacatcttc cagctcttca ccaggccggt ggcgcggctc gatccgtata cgacacccaa
     4321 cccgcgcctc ttcatctgct cggcttcgac tcctccaggc ggcggcgtgc acggcatgtg
     4381 cggctaccat gccgcgcaaa gcgccctcaa acggatcgag cgattcgagc cagagtcact
     4441 cgtctagccg agtcagccgg taaaccaata cgcccaagcg atgaacagga gctgaaacgg
     4501 caatcgcagc caaagcgccc agcgcggtat cggctttcgc gggtcgagcg agatgttgtc
     4561 ggtgagcatg tagatgttcg ccgggaacac cgcgatgtag agcgcgatga ggccccaggc
     4621 cgcccagctt cgggtgctgg ggatgagcag gccgaggcca ccgaggatct cgaagaatcc
     4681 gctgatgtag accagtgcta gcggtgccgg caggtacttc gggacgatgc gaacgaaagg
     4741 cttcgggcga acgaagtgga ggatgccgat cgcgaccatg gcgaaggcga ggaggtagag
     4801 ggcggcgagc tcgagaggag gcacgagctt gcttgtagcg tagagccgtc ccgagggata
     4861 gtgtcgatcg aatgggcaag gagcgcagca agccccttcg ggcggagcag ttcagtacag
     4921 cgcgtgccgc gcagccattt tggtatcggg ccatcaacgg cgttggccgg ctcctgccgc
     4981 gcttgggcaa tccttcggcg gaagcctggt tcgcgaaatc gaagcgacaa aacccgggcg
     5041 tgggcaaccc acgacctgag accttggcgg cgctcgatgc tctgcttcgg tcgctgcgtg
     5101 aagacgccgc tctcagcttc gcgggccgca tcgccgcgca aatggactgc ctccgcatgg
     5161 cggggcagca tctgctggtc gagcaggcct tagccgagaa accggagatc ctcgccaccg
     5221 agatcccatc accgatcttt ctgatcggct ggatgcgcac gggtacgact ttcgtccatc
     5281 gcttgctcgc ccaagatccg gacacgcgga cgatgccgta ttgggagagc atgtatcccg
     5341 cgcctccgaa gggcaccaaa gacgagcgcc ccgtgcagct ggcgcgcttg ctcgagcagc
     5401 tggagagcat ctctccgaac tatgcggcga ttcatcccat gggcgcagag gagcccgagg
     5461 agtgcgtcgc gctgttcacg aacgtcttcc gaacgcttca gtacaacgtt cagtaccggg
     5521 tccccagtta cgtgagctgg ctgcaagggc aggacccccg catcgcgtac gggcagtatc
     5581 gccagcagct gcaattggtt cattaccacc gaccgcacgg aacgcgcttc gcgctcaagg
     5641 atccgacgca ctcggtgttc gtcgagacga ttctcgagct cttccccgat gcgcgtttcg
     5701 tgttcacgca tcgcgatcca gcaacgaccc tcagctcgct ttgcagtctc tacgcgtaca
     5761 cccgggcgct cttcagcgac gatgttcatg ccgaggccct cggtccggag ctgctgaata
     5821 gctaccttcc gccaaccctc gagcatgctc tcgaggtcgt tgatggcctg cccgccggcc
     5881 gagtcgctca cgtgcgccac gtcgatgtgc gacgcgatcc gatcagcgcg atggcgagcg
     5941 cgtatcggga cctcgggatg gagctcggcg agccggcgcg cgcggcgatg cagaagatgc
     6001 tcgatgccga gagccgcaag ccccgggaca tccacattca ttcgccagag gcgttcggcc
     6061 tcagcgcaga tgaagttcga gcccggctgg cgagctactg cgcgcgcttc gacctatgaa
     6121 gcgcgcggct taccgctgag cttggctttt gcgctaccga atgcgtggcg aatcttgacg
     6181 cctgcccaga tcacgaagac gagcaagacc agcaagacga ccatgttgac ccagggtacg
     6241 tagacgtagt ctgcatcgac ttctttgagg gacagaacgt tcggatagga gtccaagatg
     6301 gcgatgcgaa gcccgtagaa tttcacgagc gcgatctttt cctcctcctt gctggccagc
     6361 ttcgatgctt cggcggcgat gtcacccgaa ttccatttca agtaccagcc gttgtcctcg
     6421 tttcgaaaga cccgcgcctt cttcgagtcg agctcggtcg catagaggaa gcgaacgtca
     6481 cgtgtcgtgc cttcgcctcc agccatgtcc gtcttgtcca ttcgtttgac gtcggttccc
     6541 gtgatccggg ccatggtcgc gcggggcatg aaataaacga gcgtgcccgc gaacagaaga
     6601 gtcaccagaa ccgcaagcgc tatacctagt tttcgcatcg atttgctcct cgcggctgat
     6661 ccatacggcc tttgtggggg cgcgcgcaag cgcgacctgt gttcagagcc ctcgcgacca
     6721 ttcgggacga agcg
//
LOCUS       DASRZF010000169         4157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3205174, whole genome shotgun sequence.
ACCESSION   DASRZF010000169 DASRZF010000000
VERSION     DASRZF010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3205174"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..2353
                     /locus_tag="VFG22_07700"
     CDS             245..2353
                     /locus_tag="VFG22_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416166.1"
                     /translation="MKYWATCLGMMTLSALLAAGCGSDSTSTPGTGGDGGSGGTGGSG
                     GAIPAPPLGCDPLTPSYCGFPYPNDYWTVPDATTVTGLRLALPEVTMPVNRNGVRSKP
                     DAFNEMDGFSPGIAAMTHFPGATVTGLATPDSIQDSLAETSPTIILNTKTGERLAHWV
                     DLDEYVVEAKRRLDDGKEPPDPKNFEITRELEELEQEQALMLRPAIRPEDATRYIVAI
                     RNVRDDAGEPVAPSPGFQALRDDAPSDDPVIESRRAHFDEIFATLEDAGIARDDLQIA
                     WDFTTSSRENNTRAMLHIRDDALARFSDGVEYSIELKNEGLIEGIACRFEITFQMPLY
                     MTQGEAGGLLNLGSDGLPEQNGFYPYAAALIVPERAKVEPAPLVEFGHGQLGAKEQVQ
                     GFQPIAASENLAAFGLDWKGFAFDDVPIVASVIFGGDMSTFRAIPERMHQGFLNFLMA
                     MRTLSREAQGGPTTKLNQALADDCGGAMLDGSKRYYFGGSQGGILGATLMALTTDVER
                     GLIAVPGQSYNLLLNRSVNFDEYSGLWYGLYGWNALDMQMNLALIQGLWDRAEPTGYS
                     KYIRTNRLPNTPAHEILIQVSKADHQVTNLGAHIMARTIGGVVNLASGDDVMNVIRPV
                     WGIDVQSGSRLGSSMIEVDFGNPDPPITNIPHWADTMDDPHGRATELIGIGATLRNFY
                     DTGVAENPCNGPCDEDDIAP"
     gene            2363..2977
                     /locus_tag="VFG22_07705"
     CDS             2363..2977
                     /locus_tag="VFG22_07705"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET6416167.1"
                     /translation="MGTSPNRVLVLFAHPALEKSRVNRAMVRGLDGLQGITFHDLYEA
                     YPSFDIDVDAEQRLLLEHDVLVLHHPFYWYSTPALIKQWEDLVLTHGWAYGSEGTALR
                     GKLMMSAITTGGSEAAYQPGGHNRFTVSELLRPIEQTARLCGMDYMPPFIVYGTHGMT
                     DDAIRGHAEDYRRVLLALKDGALDLAAARQFPHLNSNLNAVVRG"
     gene            2987..>4157
                     /locus_tag="VFG22_07710"
     CDS             2987..>4157
                     /locus_tag="VFG22_07710"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HET6416168.1"
                     /translation="MHGQGFFYQAFVYLGAAVVAVPIAKRLGLGSVLGYLIAGMAIGP
                     FGIGLIGAEGEDVMHFAEFGVVMMLFLIGLELQPSLLWRLRGPIVGLGGLQVSASALV
                     IAVIAGATGLTWNTALAVGMILALSSTAIVLQTLSEKGLSNTDGGQSAFAVLLFQDIA
                     VIPMLALLPLLASETEAGSGLAAHANQSTAWVDDLSGSAQTLAVLAAVAAIVVGGRYL
                     MRPVFRFIARTRQSEIFTAAALMLVIGIALLMSQVGLSPALGAFLAGVVLADSEYRHE
                     LEADIEPFKGLLLGVFFIAVGASVDFGLILNDPVPIAAIVGALVVVKLGVLLVLGRIF
                     GMGVDQNLLFAFSLAQGGEFAFVLFSFAAQQGVIDTETTQTLIAAVAISMTATPIL"
BASE COUNT          767 a         1266 c         1283 g          841 t
ORIGIN      
        1 tggaccaagc tccagcgccg atggtctcgg ccgttgcgcg agcggcctcc ccgtcgatgt
       61 ccgtcacgag gacggcgaat cccttcttgg cgagaccttt ggcgatttcc cgcccgagac
      121 ccttgccggc tcctgtcacg accgctgttt tcatgcctgc ctcttttgaa gcatcccggg
      181 cgaagagcaa gaggtactcg accggcactg gtaagcacac gcgaatccgt ctagcatccg
      241 ccagatgaaa tattgggcca cttgccttgg catgatgacg ctcagcgccc ttctcgctgc
      301 cggatgcggc agcgacagca ccagcacccc tggtaccggg ggggacgggg gaagcggagg
      361 cacgggcggc agcggaggcg cgattcctgc cccccctctt ggatgtgatc cgctgacgcc
      421 gagctactgc ggttttccgt atcccaacga ctactggacc gttccagacg caaccacggt
      481 caccgggctt cgactcgcct tgccggaagt cacgatgcca gtgaatcgaa atggggtccg
      541 ttccaagccc gacgctttca acgaaatgga tggcttctcg ccaggcattg cggccatgac
      601 ccattttcca ggcgcaacgg tcacgggact tgccacaccg gacagcattc aggactcgct
      661 cgctgagaca tcgcccacga tcatcctcaa caccaagaca ggtgagcgct tggcgcactg
      721 ggtggatctc gacgagtacg tcgttgaggc gaagcgccgc ttggacgatg ggaaagagcc
      781 gccggaccca aagaacttcg agatcactcg cgagctcgag gagctcgagc aagagcaggc
      841 gctcatgctc cgccccgcaa tccgtcccga agacgcgacc cgctacatcg tcgcgattcg
      901 gaacgtgcgt gacgatgcgg gagagcccgt cgctccttcc cctgggtttc aggctttgcg
      961 ggacgatgct ccatcggatg atccggtcat cgaatcgcgc cgggcccact tcgacgagat
     1021 cttcgcaacc ctggaagacg cgggcatcgc gcgtgacgac ctccagatcg cctgggattt
     1081 caccacgtcg agccgggaaa acaatacccg cgcaatgctg cacatccggg acgatgcgct
     1141 ggcgcggttt tccgacggtg tggagtactc tatcgagctc aagaacgagg ggctcatcga
     1201 gggaatcgct tgccgtttcg agatcacctt ccagatgcct ctctacatga cccagggtga
     1261 agcaggcggc ctgctgaacc tcggctcaga cggtctcccg gagcagaacg gattctatcc
     1321 ctatgcggcc gccctgatcg tgcccgagag ggcgaaggtt gagcccgcgc cgcttgtcga
     1381 attcggccac ggccagctcg gcgcgaagga gcaagtgcag ggctttcaac cgatcgcggc
     1441 ctcagagaac ctcgcggcgt tcgggctcga ctggaaggga ttcgcgttcg acgacgttcc
     1501 gatcgtcgca tccgtcatct ttggaggcga catgtcgacg tttcgggcga ttcccgagcg
     1561 aatgcatcag ggcttcttga acttcttgat ggcaatgcgg accttgtcgc gggaggccca
     1621 gggcggtcca acgaccaagt tgaatcaagc tctcgccgac gactgcggtg gcgccatgct
     1681 cgacggatcc aagcggtact acttcggcgg cagccagggc ggcatcctag gggcaacgct
     1741 catggctctg acgaccgacg tggagcgcgg cctgatcgcc gtcccggggc agtcttacaa
     1801 cctcttgctc aatcgtagcg tgaacttcga cgagtactct gggctctggt acgggctcta
     1861 cggctggaac gcgctcgaca tgcagatgaa cctggcgctg attcaggggc tttgggatcg
     1921 cgccgagcct acgggctact cgaagtacat ccggaccaat cgcctgccga atacgcccgc
     1981 acatgagata ctgatccagg tctcaaaagc cgatcatcag gtaacgaacc tcggcgcaca
     2041 catcatggct cgcacgatcg gaggagtggt taatctggcg tccggcgatg atgtcatgaa
     2101 cgtcatacgg ccggtctggg gaatcgatgt ccaaagcggt tcacgcctcg gttcgtcgat
     2161 gatcgaagtt gatttcggaa accctgaccc gccgatcacg aacataccgc actgggccga
     2221 caccatggat gacccgcatg gacgcgcaac ggagctcatc ggcatcggcg ctaccttgcg
     2281 gaatttctac gacacgggcg tcgccgagaa cccctgcaac ggcccctgcg acgaagacga
     2341 tatcgcaccc tagtctttcg ggatgggcac ttctccgaat cgcgttctcg ttctcttcgc
     2401 acatccggcg ctcgagaaat ctcgagtcaa cagagccatg gttcgcgggc tcgacggact
     2461 ccaggggatc acctttcatg atctctacga ggcttatcca agcttcgaca tcgatgtgga
     2521 tgccgagcag cggctcctcc tcgagcacga tgtgctcgtg cttcaccatc cgttctactg
     2581 gtacagcaca cccgccttga tcaaacagtg ggaagacctc gtgctgacgc acggctgggc
     2641 gtacggaagc gaagggaccg cgctgagggg caagctgatg atgagcgcca ttacgaccgg
     2701 gggaagcgaa gccgcctatc aacccggtgg gcacaaccga ttcaccgttt ccgagctact
     2761 ccggccgatc gagcagacgg cgcggctatg cggcatggac tacatgccgc ccttcatcgt
     2821 gtacgggacc cacggcatga cagacgatgc gatccgtggg cacgccgagg actatcgccg
     2881 tgttctcttg gcactgaaag acggtgcctt ggacctcgcg gcggctcggc agtttccgca
     2941 tctgaattcc aacttgaatg ccgtggtgag aggctgaggg cacgggatgc acgggcaggg
     3001 cttcttctac caagcgttcg tctacctcgg ggccgcggtc gtggccgtgc cgatcgcgaa
     3061 gcgccttgga ctcggctccg tcctcgggta cctgatcgca ggtatggcga ttggcccctt
     3121 cgggatcggg ctcatcggtg ccgaggggga ggatgtcatg cacttcgccg aattcggcgt
     3181 cgtcatgatg ctcttcctga tcggcctcga gctccaaccg tctttgcttt ggcggctgcg
     3241 cgggccgatc gtggggctcg gaggcctcca ggtttcagca agcgcgctgg tcatcgcagt
     3301 cattgcaggg gcaacggggt tgacgtggaa caccgcgctg gcggtcggaa tgattctcgc
     3361 gctttcgtct acggcaatcg tgttgcagac gctctcggag aaggggctgt ccaacacaga
     3421 cggcggacag agcgcgttcg cggttcttct ttttcaagac atcgcggtca tcccgatgct
     3481 cgcgctgctg ccacttcttg caagcgagac cgaggcgggc tcagggctgg ctgcacatgc
     3541 aaaccaatcg actgcatggg tcgacgattt gtcggggtcg gctcaaacgc tcgccgtgct
     3601 ggctgcggtc gctgcaatcg tggttggtgg ccgatacttg atgcgtcccg tctttcgatt
     3661 catcgctaga actaggcaaa gcgagatctt caccgcggcg gcgttgatgt tggtcatcgg
     3721 catcgcgctg ctgatgagtc aggtcggatt gagtccggcg cttggcgcct ttttggctgg
     3781 agtcgtccta gcggatagcg agtaccggca cgagctcgag gccgacatcg aaccgttcaa
     3841 gggcttgctt ttgggggtct ttttcatcgc agtcggagct tcggtggatt tcggactcat
     3901 cctgaacgac ccggttccca tcgcggcgat agtgggcgcg ctagtcgtcg tgaagctcgg
     3961 cgtgcttctg gttctgggtc ggattttcgg tatgggcgtg gaccagaatc tgctgttcgc
     4021 attctccttg gcgcagggcg gcgagtttgc ttttgtcctg ttctcgttcg ccgctcagca
     4081 aggtgtcatc gatacggaaa cgacgcagac tctcattgcg gctgtcgcga tttcgatgac
     4141 tgcaaccccg atcctca
//
LOCUS       DASRZF010000170         4938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2179395, whole genome shotgun sequence.
ACCESSION   DASRZF010000170 DASRZF010000000
VERSION     DASRZF010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2179395"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /gene="serA"
                     /locus_tag="VFG22_07715"
     CDS             <1..806
                     /gene="serA"
                     /locus_tag="VFG22_07715"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:NF008759.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HET6416169.1"
                     /translation="ARNAYEVRGKCLGIVGYGRIGSQLGILAEGLGMRVLYHDVEHKL
                     PLGNAMPVTTLNELLAAADVLTLHVPETPATRNMIDEQALRSMRPGSLLINAARGTVV
                     DIDALASALASGHIGGAAIDVFPVEPKSNQDTFESPLRQFDNVILTPHIGGATEEAQL
                     NIAREVVAKLVKYNDNGSTVSAVNFPQVAIPDHEGRRRILHIHRNEPGVMEQINRVFS
                     SRGINVAAQYLETRADVGYVVMDIELDDAGPLLEELEAIPATIRVRILR"
     gene            complement(809..1900)
                     /locus_tag="VFG22_07720"
     CDS             complement(809..1900)
                     /locus_tag="VFG22_07720"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET6416170.1"
                     /translation="MSNPENGSVRPEKRDESNQSVMQAAVRLSLLALLVFWCLKIVGP
                     FINPILGGVVIAIAVQTPYAKLTRALGGRPRLAAVALVAIALLVLIIPTLTLGASLVE
                     STADLSGNLMHDRIELPPPPEGLADLPLVGERLQAFWLGSSENLAAALVKLAPYLKDF
                     GLWLVSTVGNLGLGLAMFIVAIVIAGALLPNGRAASNLARKFAFMVAGERGPALANLA
                     ASSVQSVTRGVLGVAVIQSLLSGLGMLAVGVPGAGLWTLFILILAVMQLPSMIVLLPV
                     ILYVFSTSVTWIAIVFTIWAVLIGLSDNVLKPLLMGRGSSVPMLVLFMGSLGGFISSG
                     ILGLFVGAVVLSLGYTVFVAWIDDADAPP"
     gene            complement(1897..2637)
                     /locus_tag="VFG22_07725"
     CDS             complement(1897..2637)
                     /locus_tag="VFG22_07725"
                     /inference="COORDINATES: protein motif:HMM:NF023390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HET6416171.1"
                     /translation="MIEVMTDLPDRVLGVKASGEVTADDYRTVLVPAIEEKLTQHKKA
                     RLLYVLSDEFRGYSGGAAWEDAKVGMKHLTSFERVAVVTEVEWIEKMVRAFGFAIPGE
                     VRVFDDDALEDARRWISEPASKGKLSFDLLEDRGVLIMEPHGELEAADFERLSAEVDP
                     YIARAGSLRGVMIVARHFPGWDDFGALTTHIRFVREHHKKIRRVALVSADRLVSAIPR
                     IASRFVDAEVRAFPFEARDEALLWVGGP"
     gene            2706..3194
                     /locus_tag="VFG22_07730"
     CDS             2706..3194
                     /locus_tag="VFG22_07730"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET6416172.1"
                     /translation="MTNKIAANKVVTLSYTLRDDDGEIIDQSEDGSPLLYLHGAMNIV
                     PGLEEQLEGVAQGESIKATVSPEKGYGPRIGEAQEVPRNLFPADADLVAGMQVVAHDD
                     QGRQIPFFITGITEETITVDPNHPLAGETLHFEVTVVSLRDASDEEIEHGHPHGAGGH
                     HH"
     gene            complement(3207..4838)
                     /locus_tag="VFG22_07735"
     CDS             complement(3207..4838)
                     /locus_tag="VFG22_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416173.1"
                     /translation="MRSLAPAIPVILVLVCASATLADEPDAGAPAERGGSGQNAPTPE
                     NKRPRLVKPEGPGADNAQLGGEPSLTETAVEPSTQVDAGQSSDEDCDLDCLEARINKE
                     EAREQQRKSTLDLAKETDSVNIDAQDGSGDALSAEPSTTGLSGAGTESTEGNLRLARL
                     GPVRIRIGDSNDWVGIGFAVQMEFDSAQQFAGGGETKSNSTTLEFRRIRATLSSSFID
                     GRISSLFQINMTPSSFELIDMWFAFTRLKFASMRIGQFKIPYDRYRAQSFAALSFIDW
                     APTTRMFGSERQIGVEVLAKGGFLNLEYAGGIFTGVNARASHAVGITEVYGLTPMNPS
                     SLGTGEVVREFHPALVGRVAKNFGAINTDTNSDVTGEKKLRHSVGAGVAWDARPTPTQ
                     DLGLRLSAEWLGKIRHIDINVISYLAWYAPWQGGKILFGPIGFMAEVGYRFTPMWELA
                     IRYSTTYLTPWLRSDARSFGEAQIANASDPGAAQAQFGQNGNQITNDELALAGTSHIF
                     GNSLKVLAQAAWESQLWAQGRRNGLALNLQLQFLF"
BASE COUNT          999 a         1556 c         1488 g          895 t
ORIGIN      
        1 cggctcgaaa tgcctatgag gtgaggggga agtgcctagg catcgtcggt tacggccgaa
       61 tcgggagtca gctcggaatc ctggcggaag gtctcggcat gcgggtcctc taccacgacg
      121 tcgagcacaa gctcccgctc ggaaacgcaa tgcccgtcac cacgctcaac gagctcttgg
      181 cggcggcgga cgttctcacc cttcacgttc ccgagacccc ggccacgcgc aacatgatcg
      241 acgagcaagc tctccgctcc atgcgcccag ggtcgctcct catcaatgct gcacggggga
      301 ccgtcgtcga tatcgatgcg ctggcctcag cgctcgcctc gggtcacatc ggcggcgctg
      361 cgatcgatgt cttcccggtg gagccgaaat ccaatcaaga cacgttcgag tcccctctga
      421 ggcaattcga caatgtcatt cttacgcctc atatcggcgg cgccacggag gaagcccaac
      481 tcaacatcgc ccgggaggtc gttgcaaagc tcgtgaagta caacgacaac ggctccacgg
      541 tgtctgcggt gaacttccct caggtcgcga tccccgatca cgaaggtagg cggcgtatcc
      601 tgcacattca caggaacgag cccggcgtca tggaacagat caatcgcgtg ttctcgagcc
      661 gtggcatcaa cgtggccgcg cagtatctcg agacgcgcgc agatgtcggt tacgtcgtaa
      721 tggacatcga gctcgacgat gcgggaccct tgctcgagga gctcgaggcc atcccggcca
      781 cgattcgtgt tcgtattctt cgctgagtct agggcggcgc gtcggcatca tcgatccacg
      841 ccacgaagac cgtatacccg agcgagagga cgaccgcgcc cacgaacagg ccgagaatcc
      901 cgctcgagat gaatccaccg agcgagccca tgaacagcac gagcatcggt accgacgagc
      961 cgcgtcccat gagcagtggc ttcaacacat tgtcgctcag cccaatcagc acggcccaga
     1021 tcgtgaacac gatcgcaatc caagtcacgc tcgtcgagaa tacgtagagg atgaccggaa
     1081 ggagaacaat catcgaaggc agctgcatca ccgcgaggat caagataaac agcgtccaga
     1141 gacccgcgcc aggcacgcca acggcgagca tgcccaagcc ggaaagcaag gactggatca
     1201 cagccacacc gagaacaccg cgtgtcacgc tctgcacgct agacgctgcg agattcgcga
     1261 gtgcagggcc acgctctccg gcgaccatga aggcaaactt tcgcgccagg ttggaggccg
     1321 ctctgccatt cggcaagagc gcaccagcga tgacgatggc cacgatgaac atggccaaac
     1381 cgagtcccag gttgccgacg gtagacacca gccagagacc gaagtctttg aggtagggcg
     1441 caagcttgac cagcgctgcc gccaggttct ccgaagagcc gagccaaaag gcctgcaatc
     1501 gttcgccgac cagaggtagg tctgcaagtc cctctggcgg agggggtagc tcgatccggt
     1561 cgtgcatcag gttgccggat aggtcggccg tcgactcgac caagctcgcg ccgagcgtga
     1621 gcgttgggat gatgagaacc agcaaggcga tcgcgacgag cgctaccgca gcgagtcgtg
     1681 ggcgtccgcc gagcgcgcgg gtcagttttg catagggcgt ctgtaccgcg attgcgatca
     1741 cgaccccgcc gaggatcgga ttgatgaatg ggcccacgat cttcagacac cagaacacga
     1801 gcagagcgag caggctcaac cgaaccgcgg cctgcatcac gctctgattg ctttcgtcgc
     1861 gcttttcggg tcgcacggac ccattttcgg gattgctcac ggtccgccga cccagaggag
     1921 tgcttcgtcc cgagcctcga acggaaaggc gcggacctcg gcatcgacga agcgcgaagc
     1981 aattcgcggg atcgcggaaa ccagtcggtc cgcggacacc aaggcgactc gacggatctt
     2041 cttgtggtgc tcgcgtacga agcgaatgtg ggtcgtcagc gccccgaagt cgtcccagcc
     2101 aggaaagtgc cgcgcgacga tcatgacgcc tctcagggaa cccgcacggg cgatgtaagg
     2161 gtcgacctcg gcgctgagac gctcgaagtc agcggcttcg agctcgccat gcggctccat
     2221 gatcagcacg cccctgtcct cgagcagatc gaacgaaagc ttgcccttcg aagcaggctc
     2281 ggaaatccaa cgccgggcat cctcgagagc atcgtcgtcg aagacgcgaa cctcaccggg
     2341 gatcgcgaag ccgaatgccc tcaccatctt ctcgatccac tccacctcgg tcacgacggc
     2401 gactcgctca aacgaagtga ggtgcttcat tccgaccttg gcgtcctccc aggcggcgcc
     2461 ccccgagtat ccacggaact catcgctcag cacgtagaga agacgtgcct tcttgtgttg
     2521 tgtcagcttc tcttcgatcg ccgggaccag aacggttctg tagtcatctg ccgtgacctc
     2581 accgctcgct ttgaccccga gcactcggtc gggaaggtcg gtcatcactt cgatcatgga
     2641 ggactcctct cgatggaaca gcaactagca tttcgggcca agtatggcat ggtcccgtgc
     2701 aggtcatgac caacaaaatc gcagcaaata aagtcgttac gctcagctac accctgcgcg
     2761 acgacgacgg tgaaatcatc gatcagtccg aggacggaag cccgcttctc tacctccacg
     2821 gcgccatgaa catcgtgccc ggtctcgagg agcagctcga gggcgtggct caaggcgagt
     2881 cgatcaaggc gacggtttcg cccgagaagg gatacgggcc gcggattggc gaggcccagg
     2941 aagtaccgcg caatctcttc ccagccgatg cggatctcgt cgcaggaatg caggtggttg
     3001 cccacgatga tcagggccgc cagattccat tcttcatcac tggcattacc gaagagacga
     3061 tcacggtcga tccaaaccac ccgctggctg gcgaaacctt gcatttcgag gtgaccgtcg
     3121 tctcgctccg cgatgcatca gacgaagaga tcgagcacgg acatccgcac ggcgcgggcg
     3181 gccatcacca ctgagcccac gcgagctcag aagagaaact ggagctgtag gttcagggcg
     3241 agtccgtttc ttctgccttg agcccagagc tgcgactccc aggccgcttg cgccaggacc
     3301 ttgagactgt ttccgaatat gtgcgaggtg cccgcaaggg cgagctcgtc gttggtgatc
     3361 tggtttccgt tctgaccaaa ctgcgcctgc gcagcacctg gatcgctggc attggcgatc
     3421 tgcgcctcac cgaaggatct agcgtcgctt cgaagccagg gtgtcagata tgtagtactg
     3481 tagcggatcg ccagctccca catcggcgtg aagcgatacc ctacctctgc catgaacccg
     3541 atcggaccga agaggatctt gccgccctgc caaggtgcgt accaggcgag gtagctgatg
     3601 acgttgatgt cgatgtggcg gatctttccg agccactccg cggagagccg gaggcccaag
     3661 tcctgcgtcg gtgtcggcct tgcatcccag gcgaccccgg cacccacact gtgtcggagc
     3721 ttcttctccc cggtcacatc gctgttggtg tcggtgttga tcgctccgaa gttcttggcc
     3781 acacggccta cgagcgcggg atggaactcg cgcaccacct ctcccgtccc cagcgaagaa
     3841 ggattcatgg gagtcagccc gtagacttct gtgatcccga ccgcgtgcga agctcgagca
     3901 ttgaccccgg tgaagatgcc gccagcgtac tcgaggttca ggaagccgcc cttggccagc
     3961 acctcgaccc caatctggcg ctcggagccg aacattctcg tggtgggcgc ccagtcgatg
     4021 aacgaaagtg ccgcgaagga ctgtgcccgg tagcggtcat acgggatctt gaactggccg
     4081 atgcgcatag acgcaaattt cagacgcgtg aacgcgaacc acatgtcgat caactcaaaa
     4141 gagctcggcg tcatgttgat ctgaaagagg ctgctgatac ggccgtcgat gaacgagctc
     4201 gacagagtcg cccggatccg gcgaaattcc agtgtcgtcg agttgctctt cgtttctcct
     4261 ccacccgcga actgttgagc ggagtcgaat tccatctgca cggcgaaacc aattccaacc
     4321 cagtcgtttg aatcgccgat ccggatgcga accggaccca gacgcgccag ccgtaggttc
     4381 ccctccgtac tctccgtccc ggcaccagaa aggcctgtgg tgctcggctc ggccgaaagg
     4441 gcatcgccgg aaccatcctg cgcgtcgatg ttgaccgagt cggtttcctt ggcgagatcg
     4501 agcgtactct tgcgttgctg ttctcgcgct tcctccttgt tgatccgagc ttcgagacag
     4561 tcgaggtcgc agtcctcgtc ggagctctgc ccggcgtcta cctgagtgga gggttcgacc
     4621 gcggtttccg tcagcgaggg ctcgcccccg agctgcgcgt tgtccgcgcc aggcccctcg
     4681 ggtttcacga ggcgtgggcg cttgttttcg ggagtgggcg cgttctggcc gctaccgcct
     4741 cgttccgctg gcgcgcccgc atccggctcg tctgccagcg tggccgaggc gcacacgagc
     4801 acgaggatca cggggatcgc tggggccagg gaacgcatcg gctgcagaat gccagcctat
     4861 gagggttggg agctagtttt ccaaggtgct gaggttgagt tcgtcgatct tcgcggcctg
     4921 gatgtccgca tcgcagta
//
LOCUS       DASRZF010000171         4331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3210215, whole genome shotgun sequence.
ACCESSION   DASRZF010000171 DASRZF010000000
VERSION     DASRZF010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 4331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4331
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3210215"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1614
                     /locus_tag="VFG22_07740"
     CDS             <1..1614
                     /locus_tag="VFG22_07740"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET6416174.1"
                     /translation="SFQQPLIAPLYGGRNPLEILAAFAGEKNPNAHDEVQATQKDTLQ
                     KMTVSLLMLGFPPDGVSSDAVWRGGLHSGVFVQPQQGEAKVLLNPILDALASSPARRT
                     PVGDGNFEVNFEPDPALWDGTHANNLWALELPSPMTKLVWDNAALISPATSRALGVKN
                     GQMLRISKGDAKVEVPAWIVPGHADNCVTLPLGWGRTAAGRYGNGKGFDVNPLRSSDG
                     MDFTDGVSIEPLREGYNLVQTQTHDRMEGRPIAIDATLDEYKKEPDFASYRSVDPVST
                     PPLWKQQDYSQGHKWGMAIDLNGCTGCNACVVACQAENNIPTVGKFEVERGREMHWLR
                     IDRYFVGDDENQPEVAVQPVTCQQCEEAPCENVCPVNATVHSPEGLNDMSYNRCVGTR
                     YCANNCPYKVRRFNYLDWHGHLDDKFGWYEDFPESRKLQFNPNVTVRMRGVMEKCTYC
                     VQRIEEGKIAAKREGRMLKDGDIKSACQQACPAQTIAFGDLNDPNSTVAKWSAVDRNY
                     KLLAEIGTRPRTTFLARIRNPNPKLKGNG"
     gene            1621..3078
                     /gene="nrfD"
                     /locus_tag="VFG22_07745"
     CDS             1621..3078
                     /gene="nrfD"
                     /locus_tag="VFG22_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HET6416175.1"
                     /translation="MSYDAPIKELGETSLLPEGTTANDVTETIAGVTENPAPRGWWLL
                     FLLSVSVLGVMGAALSYLFIQGVGVWGNNAPIYWAWDITNFVWWIGIGHAGTLISAIL
                     FLFRQKWRTSINRFAEAMTVFAVLCAGLFPAIHTGRPWFDYYLFPLPNQMDMWPNFKS
                     PLMWDVFAVSTYLTISVIFWYVGLIPDFATLRDRSKNLTRKRVYGFFALGWRGAHRHW
                     QHYERAYLIFAGISTPLVLSVHSVVSFDFATSVVPGWHTTIFPPYFVAGAVFSGFALV
                     MTLMLICRSIFKLHNIVTIRHLDLMNKFMLGTALLVGYAYAMEFFIAWYSSNPYEQWV
                     FFHNRLGWGQLDSKGNWGPLWWAYYAMVFCNVIVPQIFWFKRARTSVPIMLIASVLIN
                     IGMWFERFVIIVTSTYQDFLPSSWGMFHPTAIDILTYLGTFGLFFTLFLLFVRWIPMI
                     AIAEVKGNLPGAHPDLHAIQAHEEPGAAAVPVPAE"
     gene            3087..3665
                     /locus_tag="VFG22_07750"
     CDS             3087..3665
                     /locus_tag="VFG22_07750"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HET6416176.1"
                     /translation="MANNTKKPALYLAEYDTPNAIAQAAMKVRDAGYKIWDCHTPYAM
                     HGMDEAMGLAPTRIGVVSFIFGMLGVATAVFMMQYTNAFSFNLMNIGAGYPLVVGGKP
                     PGAFPSMVPIMFELGILLTGFATLFGLLGLIKLPMHNHPIFESDRFEAATDDKFFISI
                     EASDEKFDLERTKALLEATHPTYLELVEEEVQ"
     gene            3665..4288
                     /locus_tag="VFG22_07755"
     CDS             3665..4288
                     /locus_tag="VFG22_07755"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET6416177.1"
                     /translation="MRSLWIAFTFAALAAGCIGAESSEPPVVGIRNMYNQPRYDTQER
                     QPFFADQRSMRPQVEGTVSREMSKGFDVATGRSAQGNKWIEKVPRGVIAGFGSREAIM
                     ARGKDRYEIYCSVCHSISGDGQGMVSRRAAALGATALMAPSFHDDRLRHIPDGQLYAT
                     ITNGVRNMPAYAHNLPVDDRWAIVQYVRALQLSQAPAAAPEKTEQSQ"
BASE COUNT          855 a         1355 c         1265 g          856 t
ORIGIN      
        1 tcatttcagc agcctttgat cgcaccgctc tacggtggcc ggaacccgct cgagatcttg
       61 gccgcgttcg ccggcgaaaa gaaccccaat gcccacgacg aggtacaggc gacacagaaa
      121 gacacgctcc agaagatgac cgtgtcgctg ctgatgctgg gcttcccgcc cgatggcgtg
      181 agctcggatg cggtctggcg tggcggttta cactcgggcg tcttcgtcca gccacagcag
      241 ggcgaagcca aagtgctcct caatccaata ctcgacgcgc tggcttcgtc gcccgctcgg
      301 cggacgcccg tcggcgacgg caatttcgag gtgaacttcg agcccgatcc tgcgctatgg
      361 gacggtacac acgccaacaa tctctgggcg ctcgagctgc cgagcccaat gaccaagctg
      421 gtttgggaca acgccgccct catctccccg gcgacgagca gggcgctagg cgtgaagaac
      481 ggccagatgc tccgcatcag caagggcgac gccaaagtcg aagtgccggc ctggatcgtt
      541 cctggacatg ccgataactg cgtcaccctc ccgcttggat ggggtcggac ggcagctggt
      601 cgctacggaa acggcaaggg cttcgacgtg aacccgcttc gcagcagcga cggcatggac
      661 ttcacggatg gggtctccat cgagcccctt cgcgaagggt acaacctcgt tcagacgcaa
      721 acgcacgatc gcatggaggg ccgcccgatc gccatcgatg cgactctcga cgagtacaag
      781 aaagagcccg acttcgcctc gtaccgttcg gtggatccgg tcagcacgcc gccgctctgg
      841 aagcagcagg actacagcca agggcacaag tgggggatgg cgatcgacct caacggatgc
      901 acgggctgca acgcttgcgt cgtagcgtgt caggccgaaa acaacatccc caccgttggc
      961 aagttcgagg tcgagcgcgg ccgcgaaatg cattggctcc gcattgatcg ctacttcgtc
     1021 ggcgatgacg agaatcagcc cgaggtggct gtccagcccg ttacctgcca gcagtgcgaa
     1081 gaggcgccat gcgagaacgt ttgcccggtc aacgcgaccg tgcactcgcc cgagggcctc
     1141 aacgacatgt cgtacaaccg ttgtgtgggc acgcgttact gtgcgaacaa ctgcccatac
     1201 aaggtccggc gcttcaatta cctcgattgg cacggccatc tcgacgacaa gtttggctgg
     1261 tacgaggact tcccagagtc gaggaagctt cagttcaacc cgaacgtcac tgttcgcatg
     1321 cgcggcgtga tggagaagtg cacctactgc gtgcagcgta tcgaagaggg gaagatcgcg
     1381 gccaagcgtg aaggccgcat gctcaaagat ggcgatatca agagcgcgtg ccaacaggct
     1441 tgcccggctc aaacgatcgc gttcggagac ctaaacgatc cgaacagtac ggtagccaag
     1501 tggtcggcgg tcgaccgcaa ctacaagttg ctcgccgaaa tcggaacacg acctcgtacg
     1561 acgtttctcg cacggatccg caacccaaac cccaagctga agggaaatgg gtaagaacca
     1621 atgagctacg acgcacctat caaagaactc ggcgaaactt cccttcttcc ggaaggcacg
     1681 actgccaacg atgtcaccga gacgatcgca ggcgtgacgg agaacccggc cccgcgtgga
     1741 tggtggctcc tgtttctcct ctcggtgtct gtgctcggag tcatgggcgc tgcgctctcg
     1801 tatttgttca ttcaaggcgt cggcgtctgg ggcaacaacg ccccaatcta ctgggcctgg
     1861 gacatcacca acttcgtctg gtggatcggt atcggccacg ccggaacgct gatttccgcg
     1921 attctcttcc tgttccggca gaagtggcgg acgagtatca accgctttgc cgaggcgatg
     1981 accgtcttcg cggtgctctg cgctggactc tttcccgcga ttcacaccgg tcgcccctgg
     2041 ttcgattact acctgttccc tctgcccaat cagatggaca tgtggccgaa cttcaagagt
     2101 cctctgatgt gggacgtctt cgcggtcagc acctacctca cgatcagcgt gatcttctgg
     2161 tacgtgggcc tgattccgga cttcgcgacg ctgcgcgatc ggtccaagaa cctcacgcga
     2221 aagcgcgtat acggtttctt cgctctaggc tggcgcggcg cccatcggca ctggcagcac
     2281 tatgagcgcg catatctgat cttcgccggt atctccacgc cactggttct ttccgtccac
     2341 tccgtggttt cattcgactt cgccacctcg gtcgttcctg gctggcatac gacgattttc
     2401 ccgccgtact tcgtcgccgg cgccgtcttc agcggctttg cgctcgtcat gacgctgatg
     2461 ttgatctgcc gctcgatttt caagttgcac aacatcgtaa cgattcggca cctcgacctg
     2521 atgaacaagt tcatgctcgg gacggcgctc ctggtcggat acgcctatgc catggagttc
     2581 ttcatcgcct ggtactcctc caacccttac gagcagtggg tgttcttcca caacagactc
     2641 ggttggggcc agctcgacag caagggcaat tggggtcccc tctggtgggc ttactacgcg
     2701 atggtgtttt gtaacgtcat cgtgcctcag attttctggt tcaagcgagc gcgcaccagc
     2761 gttccgatca tgctcatcgc gagcgtgctc atcaacatcg gaatgtggtt cgagcgcttc
     2821 gtcatcatcg taacatcgac ctaccaggac ttcctcccgt cctcctgggg catgtttcac
     2881 ccgacggcga tcgacatctt gacgtatctc ggcaccttcg ggctgttctt cacgctcttc
     2941 cttctcttcg tgcggtggat tcccatgatc gccatcgcag aggtcaaagg aaaccttccg
     3001 ggagcgcatc ctgacttgca tgcgattcaa gcacatgagg aaccgggcgc ggctgccgtg
     3061 ccggtgccgg cggagtgagg tgaatcatgg ctaacaacac gaaaaagcct gccctttacc
     3121 tggccgaata cgacacgccc aacgcgatcg ctcaggcggc gatgaaggtg cgcgacgcgg
     3181 gttacaaaat ctgggactgc cacacgccat acgccatgca cggcatggat gaggccatgg
     3241 gtctcgcgcc aacccgcatc ggcgtcgtct cgttcatttt cggaatgctc ggcgtcgcga
     3301 ccgcggtctt catgatgcag tacacgaacg cgttctcctt caacctcatg aacatcggtg
     3361 cgggataccc actcgtggtg ggtggcaagc cgcctggggc gttcccttcg atggtgccca
     3421 tcatgttcga gctcggcatt ctcttgaccg ggttcgccac gctgttcggc ttgctgggtc
     3481 tcatcaagtt gccgatgcac aaccacccga tcttcgagtc cgaccgattc gaagcagcta
     3541 cggacgacaa gttcttcatc tccatcgagg ctagcgacga gaagttcgac ctcgagcgaa
     3601 cgaaggcgct cctggaagcg acccatccga cctacctcga gctcgtcgag gaggaggtgc
     3661 agtgatgcgt tctctttgga tcgcgttcac gtttgccgcc ttggccgcgg gctgcatcgg
     3721 cgccgagagc agcgagccgc ccgtcgtcgg tatccgcaac atgtacaatc agcctcggta
     3781 cgacacgcag gagcggcagc cgttctttgc agaccagcgc tcgatgcgac ctcaggtcga
     3841 ggggacggtc tcccgcgaaa tgtcgaaggg attcgacgtc gcaacaggac gttccgcgca
     3901 gggcaacaag tggatcgaga aagttcctcg cggcgtgatc gcgggctttg gaagtcgtga
     3961 agcgatcatg gcgcgaggca aggaccgcta cgagatttac tgctcggtgt gtcactccat
     4021 ttcgggggac gggcagggca tggtctcgcg gcgagccgct gcgcttggcg ccaccgcact
     4081 catggcaccg agcttccacg atgacaggct tcggcacatc cccgacggcc aactctacgc
     4141 gacgatcacc aacggcgtgc gcaacatgcc cgcgtatgcg cacaacctcc cggtcgacga
     4201 ccgctgggcc atcgtccagt acgtccgcgc gctacagctg agtcaggcgc cagccgccgc
     4261 gcccgagaag acggagcaat cccagtgacg gcagcaaaga agaacgacta tcgcgttgga
     4321 aacgatgccg t
//
LOCUS       DASRZF010000172        20024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2868365, whole genome shotgun sequence.
ACCESSION   DASRZF010000172 DASRZF010000000
VERSION     DASRZF010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 20024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20024
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2868365"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VFG22_07760"
     CDS             <1..525
                     /locus_tag="VFG22_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HET6416178.1"
                     /translation="EAVERGASTVDRGVTVSAEAERALKKILESSKQSTAMVRGIARA
                     TVEQAKGSRQVTDAIGRIAETVQQIAAATAEQARGSELIMKSADNMRLITQQMERSSQ
                     EQAEGGRQMTGAIENISDMVLQLHQSHRKHSESSEQTLAAANRIQDLTREYERRVRDM
                     HRAVERLRQIRFGS"
     gene            621..1022
                     /locus_tag="VFG22_07765"
     CDS             621..1022
                     /locus_tag="VFG22_07765"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HET6416179.1"
                     /translation="MFGIGPTELAFFLVIVLLAVGPDKLPAFMRTVGKGLRQVRNASR
                     EFKEAIGLDELMREGDPFRAPPLRPPATRPVPLGDKPPVPSPDAIDAADTPPAPDAAP
                     IDDASPATDTPSSGEPGSAANSEAPDEAKGP"
     gene            1019..1819
                     /gene="tatC"
                     /locus_tag="VFG22_07770"
     CDS             1019..1819
                     /gene="tatC"
                     /locus_tag="VFG22_07770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HET6416180.1"
                     /translation="MTSAEIQVPEPEPRAVPEEDQEMTFFQHIAELRTRLLRSILGLI
                     PAVAISWVFKKEIKDFLVAPLQPAWEKLNLPGQAVLRFSGPADAFMMYMQNSLIAGVL
                     LASPWIFWQLWMFIAPGLYKKEKALAIPFIVASTICFVGGAVFGYKLVFPEAFTVLLG
                     FSDETLVATMFVKDYMPFFRRMLLAFGVVFEVPVLVTFLAGAGIVTWQQLIKFSRWWI
                     VCAAFLAMILTPADVYSMVMMLIPLVLLYYIGVGCAYLISVFRKKPSP"
     gene            complement(1816..2646)
                     /locus_tag="VFG22_07775"
     CDS             complement(1816..2646)
                     /locus_tag="VFG22_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6416181.1"
                     /translation="MDQVYADGLFADEVVLITGGGTGIGLVAATEMGLLGAKIAICGR
                     RPEPLRNAVTELQSRGIDAFGAPCDIREPEAITAYVDAVLERFARIDVLINNAGGQFP
                     TTAEALSPKGFAAVIRNNLVGTWSMTHAVATKAMIPQKRGRIVNVIAQIIRGFPGMVH
                     TGAARAGVDNMTKTLAVEWALHGIRVNAVAPGVIVTSGTKQYPPELLETAEKANPLKR
                     LGTAEEVSHLITYLASRYADFVAGQTFYIDGGASIWGDQWFLPEDLPKYPPYPVPERG
                     "
     gene            complement(2691..3080)
                     /locus_tag="VFG22_07780"
     CDS             complement(2691..3080)
                     /locus_tag="VFG22_07780"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6416182.1"
                     /translation="MTAKKTILVLDDDPSVRHMLQVLLEQDGHAVVAAGDVRSALAQC
                     KVALPDLMVVDLMLPIEDGEMFLREFHRRWRHSEVPVILLSASTARNDIARRLNVEAT
                     LAKPFFAQDLRELVATLVTQSEAVAAS"
     gene            3159..3746
                     /locus_tag="VFG22_07785"
     CDS             3159..3746
                     /locus_tag="VFG22_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416183.1"
                     /translation="MTSALRASVMFSCLLVACAGSEPRPSSLDSIELSEFFPLHAGNA
                     WSYDVDTGEPSTTLGVTRVEAFDGHLAEVRTGRAMVRYELLAEGIRVPPEDVWLLRAP
                     LEEGASWPARGGRTAQIISIDTRIETLAGKFERCIEVSETGGKLELEIRIVYCPGVGP
                     VALDSTMRSETSDRVVTVFARLRGYEVSRESRSGP"
     gene            complement(3718..4143)
                     /locus_tag="VFG22_07790"
     CDS             complement(3718..4143)
                     /locus_tag="VFG22_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416184.1"
                     /translation="MAYPGNDDEGRPLQTVGRVLDSRWMMAFAVLAVLVAIAWALIER
                     EPIGGDGSIAQGDARMATVELLGVPAGAEVTLDGRRIDNTLFGVSPGTRHALEVTDGD
                     GRSWRQVFLVDGSLSLVVQLRTHFVEVDVSAKAPSETRD"
     gene            4242..5165
                     /locus_tag="VFG22_07795"
     CDS             4242..5165
                     /locus_tag="VFG22_07795"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6416185.1"
                     /translation="MTKAILEARGLTKAYGNRVVVDHLDLRCRRGTVLGLLGPNGAGK
                     TTTLRMLYGFVPPDEGSISYAGQQFSVNRAEAKRMIGVCTQEDSLDYDFTVAENLFIY
                     ASYFRPRVEGLERRVTDLLDRFELTPYANVSPHVLSGGFMRRLLIARSIVHRPSVLFL
                     DEPTTGLDPSARLDVWELIDGMRAEGLAIVLTTHYMDEAERLSDELLVIHEGQNVAHG
                     EPHRVIDSVLGDHVIIVPRSEPAREEIATWLRDRGAEQVATVLGELRVPVSKDDLAEL
                     SARFTGATWMVRPPNLDDLFIALARRKARHS"
     gene            5162..5980
                     /locus_tag="VFG22_07800"
     CDS             5162..5980
                     /locus_tag="VFG22_07800"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6416186.1"
                     /translation="MSGLRNLAAVSDWRSWAVFRRNTLVFLRNWRTALIPPAMEPVIF
                     FVAFGLGFGGFIEDLNYHGVEINYATYVSAGLIAYTGFGTPYYEALYSAYVRMFYQKT
                     WDGLLATQVELPNVVWGEILWGATRGMVNISIVSSVVAVFDLLGFVEVKVEFLLLMPL
                     LGFVAGSAFASFALIFTAIVPAIDHMNYPVFLIGWPLSVVSNTFFPIEPQSQWLFALM
                     QLNPVYQLAECARGLLVLGSPGWHLLGLLGSTALFLIPCTLISIRLTRRRVLGD"
     gene            6074..8536
                     /gene="gyrB"
                     /locus_tag="VFG22_07805"
     CDS             6074..8536
                     /gene="gyrB"
                     /locus_tag="VFG22_07805"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012535740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HET6416187.1"
                     /translation="MADPPIAEQRPAAHGYDQDAIQVLEGLEAVRKRPGMYIGDPHDG
                     SGLHHLVWEAVDNAVDEHLAGHCDAVQVIVHSEGSVTVIDNGRGIPVGMHSKGVSAAE
                     VVMTQLHAGGKFNSDSYSVSAGLHGVGVSAVNAVSEWLKMEIRREGKLWFQEYERGVP
                     KAPLRAIGTSQKTGTKVTFKPDPQIFTMTDFSFEVLNNRLREISFLNAGLEVSLEDER
                     GDGKKVMHKFEGGIREFVEALNKKKTPLHDDVIYLHAVKDGVDVEIAMQWSDSYNESI
                     FPYTNNVFNRDGGTHLTGLRTALTRVINQYGSREKLLKDLKNPLSGEDVREGLTAIIS
                     IKHPDPAFSSQTKDKLVSSEVKGIVESILNEKLGQYLEEDPKAGKRIVEKTVLAARAR
                     EAARRAREMVQRKGVLDASTLPGKLADCQSKDATESEIYIVEGDSAGGSAKQGRDRRF
                     QAILPLRGKILNVERARFEKMISSAEIGTLITALGCGVAGGENFDIEKLRYHQVIIMT
                     DADVDGSHIRTLLLTFFYRQMPQLIDKGYLYIAQPPLYKVKKGKKLRYLQHDDALSRH
                     LIELGTDGIQLSMKGGGAELTGEPLRNLVKDMARFRLLLDNIGRRFDALVVEALVTAN
                     DLEVEDLADREKVEAGIEALKKYVESKRPGEIDTTIQYDEEHDRHRVIIETKDSGARR
                     VTTVDFDFLSAGNIAELRQIYQGIRALGEPPFVLTVLDKDGRPTGEPSEVPDLESLWD
                     TIDARARKGLGIQRYKGLGEMNPEELWETTMDPETRTLLQVRIEDALEAEELFSVLMG
                     DQVEPRRAFIESNALNVKNLDI"
     gene            complement(8533..10716)
                     /locus_tag="VFG22_07810"
     CDS             complement(8533..10716)
                     /locus_tag="VFG22_07810"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6416188.1"
                     /translation="MNRGPAADTLVWMGVGQKEESAWEQGFAPSTEAVAAHEPLDAGQ
                     RWAMFNATTARHVVTMFLSAGLAYLGLSFGQWSSDVDQTRLKLMTIYGLTGVAMLALS
                     WRFKRQAPPRMWSVHIGGALFLVVTSTVTICYGLSGEPSDFYLYVLIQVIAGAVIHSR
                     RWVIAIMILGDLGWGLTSLSVEGVDWGRSIGYLAGFSVVALALNHVRRQTLVTVEERR
                     LAAERASEAKTEFMANLSHEIRTPMNGVLGLSALLLDTNLDDQQTKMIMAIRESADAL
                     VGIVDEVLTFSQLQKGQVVLDQVPFDLTAVVDGVISLMRPRAEAKGLSLDADLRGFSD
                     RRFVGDAGRIRQVLLNFVNNAIKFTDVGSVLVSAKVVRRSETTRVRLSVRDTGIGIPE
                     ESLSRIFTRYHRNETSPKSSAVGNGLGLAITKQLVDLMGGEVGVQSSLDQGALFWVEL
                     DLAPGREDTLRVADPGGAGEVLIREGMRALVAEDNPTSRMVTEALLKKLSCEVDIAVD
                     GVEALAKAKSNPYDVVFMDCRMPNMDGFQATERIRKSGVREGLPIIALTASVSEGDRI
                     RCLKAGMNDTIGKPVRISMLARALERWVPVSGRPSARPVSSLPPPSALDLEMVRRLVS
                     LDGEDDDFIEEVMMGYVVQLKESMLKLGDALEAQDLDTVGLIAHSIKGASKQIGATKV
                     GELLGAIERETSVDAVRQLLGLVDQEVPRVDEAVQALLRRSRRAS"
     gene            complement(10713..11528)
                     /locus_tag="VFG22_07815"
     CDS             complement(10713..11528)
                     /locus_tag="VFG22_07815"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET6416189.1"
                     /translation="MPNEEDSERALEVAERAAREAGGLVMRAWRSVSEVSKKGRFDLL
                     TEYDLESERLIRKELSSAFPSHRIVGEEGEETGEGEMVWYVDPIDGTTNFAHGHFFFA
                     MSIALYRGSEGLIGVVHAPALDVTWKATRGRGAFRNDSPCSVSKRARLEEALCATGFP
                     YDRWSNPDNNHAELALFLQRARGIRRCGSAAIDLCLVADGTYDLYWEQSLNAWDMCAG
                     ALIVQEAGGRLSTYEGDAADPRTGKLIASNGLLHEAAVRTIREARHKLQPHKP"
     gene            11682..13385
                     /locus_tag="VFG22_07820"
     CDS             11682..13385
                     /locus_tag="VFG22_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HET6416190.1"
                     /translation="MPDKDTVTPSIRNPFKVDRDPSLPSDKPEAQRPYDEAVREGEQL
                     RARPRKAAGPVQIGKQHDKRRMTVWERMDVLRDPGTEPTILYQNWGPNLDGASIVTAV
                     IEVEGREVALYGHDFTVRAGSMDATNGTKLANLIRLAGRQGMPLVGMNDSAGAFVPAG
                     IGGLDGYAEAFTALRKISGVVPSIMCMFGYNAGGGAYLPRQGSFMIQPSNTFFGLTGP
                     GVVKSVLGEDVTADELGGPGVHGQSGVVDLTVRDEVGALRTARRLLAYLPGNNHEFAP
                     YQETSDPIDRPTGEIDTLLRKAFNSPTGFNTPIDVSIILQQICDHGDYFELQPQRARN
                     TITAFGRLGGHVVGFVCNNSAVASGQIDVDAAFKNSRFIRFCNLYNIPVIFMEDTTGF
                     LPGRDQEGRGIVQAGRAMLDSIIDLRTPRLLLIIRNAFGGAYAAFNCYATGADHVMAL
                     PTTRVAVMGPAGKEFVYKQEMRDTRTKAKLRVQEAIAAGASKNDAEAEAAAWLKEQDA
                     AFNARYERELMNPKEALSLGSISEIVMPTDVRAALAKNLNFYLSHYTPEPLQAVQREF
                     H"
     gene            13385..16189
                     /locus_tag="VFG22_07825"
     CDS             13385..16189
                     /locus_tag="VFG22_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HET6416191.1"
                     /translation="MDSDNYLHNPLIHRDRRLGESESEWTRSFACEDMTVLIVCRGPV
                     RQEALDTFSEMGMTHVGILLSEKDSIVYPRALAPELRRLDPSRVHAVSDYTGASKEER
                     TQRIAQIIKIAKKNGYDYIFAGYGFMAEDEDFVRSIEEAGLRFIGPCSHTVQAAGFKD
                     EAKRTAERVDVSVTPGINNATARLLLRKHPDLKALSKLAEKHSLEVAELSDGALELET
                     VADGVLAASYDAGIDLYTVDELGEQIELEVASILKKYEGSRIRLKAIGGGGGKGQRIV
                     NAAKQAPAMVREILGEVKALGVGDNKNILVELNIETTRHNEIQLLGNGEWTVSLGGRD
                     CSLQMHEQKLLEVSITQETLAAAVERERAAGNEAHAAAIEQDLDILRRMEAEAERFGE
                     AVKLDSASTFECIVDGARHYFMEVNTRIQVEHRVSELCYALRFRNPEDRDEFFDIHSL
                     VEAMALLAKHKVRLPRPERVQRHAAAVEARLNATDQSLSPHAGGEIMSWSDPIEGEVR
                     DDQGICFKNPDTHLFMRYKLAGAYDSNIALLVTTGEDRRDSYERLREVLRRTVIRGPD
                     LQTNLQFHYGLVHWFLANGVDAKPTTRFVVPYLTLAGELLHESNEIDVDYAYDRLCRK
                     YLSAVSTDPAAAKATTDLSVLKKTLLGRPLGRILRQPHLLSAWLSANKNHFEVKKGRV
                     TWLRNPLSVLADTYFLLHMDAEVDLPAAHRIWEQDQTVLRDGAAFYEAVEERLGSRPW
                     PELDQALKAKKPPKGFDAALWQEVREAHLGFQLGMELYSLLPLIGDKVGFFDLRVNDD
                     LTISIPERLLDSAHQAKMKKVLVPPPVNKADEIVAVSGGMYYAQEAPGMPHFIEEGQH
                     FEIGDPLYIVEVMKMFNKVYAPFAGTVDKILVETNGTIVSKGQALFKVTPDEIFVEED
                     PVEVRARIQSNTEQYLSVVA"
     gene            complement(16203..17258)
                     /locus_tag="VFG22_07830"
     CDS             complement(16203..17258)
                     /locus_tag="VFG22_07830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET6416192.1"
                     /translation="MRIHTLRGPKLEESVLGASSSRARSVTRVLEEALEASVLAVRAT
                     AGAIYIGQNVDCLRCVVSHGPDAKSLLERGGPPHPDLAARALGRRTPFVVPSSREDSE
                     LSIHAADETWTALLASLHDGGRPFGFLQLTDPEPPFRFTERETRIVEGLSRYITTSLI
                     QTSSLRRQEGLAAHDPLTGLRNVRELNNELTKAVSSARRGQRDMAIMFVDVDHLKRVN
                     SKLGHAAGSEALKRTGKALEEAAAGVGEVFRFGGDEFVVVQKRASKESARALADHMRS
                     HVAASTGGAMRPGGTLPKITVSVGVATLLGLRDLNGQPPSDLKSRLMTTADRALFRAK
                     GAGRNRVVLATPRDDRR"
     gene            17424..18392
                     /gene="pdhA"
                     /locus_tag="VFG22_07835"
     CDS             17424..18392
                     /gene="pdhA"
                     /locus_tag="VFG22_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HET6416193.1"
                     /translation="MPNKDDLLRKYRVMMLIRRFEEEAARHYAKGNIGGFLHLYIGQE
                     AIAVAAADVLQPQDYIFQTYRDHGIALARGMSPNSAMAELFGKDTGCSRGFGGSMHFF
                     DVERGFLGGWAIVGGHLALAAGTAFKSKYNGEDRVTICFFGEGSTNIGGFHEAMSLAG
                     LWKLPIVFVCENNQYAMGTPLERTSPLRDLSIKAEGYGMMGDHLEGHDVDVVQAHLAK
                     AVDRARTGGGPTFVEIKTYRFRGHSMSDPAKYRTRTELEQRKQQDPVTVVRERLLGMG
                     ADETVLDDIDLEVDETLDAAVRFAEESEPAREELMMSTALAPEGDS"
     gene            18389..19375
                     /locus_tag="VFG22_07840"
     CDS             18389..19375
                     /locus_tag="VFG22_07840"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HET6416194.1"
                     /translation="MRELRYREALREAMLEEMERDERVFLMGEEVGHYQGAYKVSEGL
                     LEKFGEKRVIDTPIAEAGFAGVGIGAAMVGLRPIIEFMTWNFSFVAFDQIISNAAKVH
                     QMSAGAIRVPIVFRGPNGAAKQVGSQHSHSCAPFYTSIPGLYVIQPSTPRDAKGLLKS
                     AIRSNNPVIFLEGETLYGVKGEVPDEGVEELIPIGESRLARPGADCTIVSWGRPYYTV
                     LGAVEKLAAEGIDCEVIDPRTLRPLDTKLMVESVKKTNRCVVVHEHWPYGGPGAEIVD
                     RIQREAFDYLDAPVERVTGLDVPMPYALNLEDKVLPTEERVIDAVKRVSYVK"
     gene            19379..>20024
                     /locus_tag="VFG22_07845"
     CDS             19379..>20024
                     /locus_tag="VFG22_07845"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HET6416195.1"
                     /translation="MAKIIGLPKLSPTMEEGTLVAWIKKEGDEVEVDDLLAEVETDKA
                     TMEFRSFDRGVLLKILVKEGETLEPDVPVAIIGKAGEDIGELLAQVEARAGTAAGTAA
                     EPAAAPESAAESAAVSEPAAVSEPAAVSAPAAVVEMESSDRILSSPLVRRLAREKSID
                     LRAVEGSGPRGRIIKRDIDSYEERPSSARTYSGERLPPRVLQASSMRRTIARQLT"
BASE COUNT         3889 a         6161 c         6200 g         3774 t
ORIGIN      
        1 gaagcggtcg agcgtggagc gagtaccgtc gaccgaggtg tgacggtgag cgccgaggca
       61 gagcgtgcgc tcaagaagat cctcgaatcg tcgaagcagt cgacggcaat ggtacgtgga
      121 atcgcgcgcg ctacggtcga gcaggccaag ggctctcgcc aagtgacgga tgcgattggt
      181 cgtattgcgg agacggtcca gcagatcgcg gcggcgaccg ctgaacaggc ccgtggatca
      241 gagctcatca tgaagagcgc ggacaacatg cggttgatta cgcagcagat ggagcgcagc
      301 agtcaggagc aggccgaggg tggccgccag atgaccggcg cgatcgaaaa catttccgac
      361 atggtgcttc aactccatca gtctcaccgc aagcattccg agagctcgga gcagacgctt
      421 gccgcggcca accgcattca ggatttgacc cgcgagtacg agcggcgcgt tcgcgacatg
      481 cacagggcgg tcgaacggct ccgccagatt cgcttcggct cgtgattgtg gtgatcaaga
      541 ggccgcgctc gttcgagcgg cccgcttccg ccatgcctct ggttgcctcc cgggggggca
      601 agcattcatc ttaggcacac atgtttggga ttgggccgac ggagttagcg ttctttctcg
      661 tgatcgtctt gttggcggtg gggccggaca agctgccggc attcatgcgc accgtgggaa
      721 agggccttcg ccaggtccgg aacgcgagtc gggagttcaa ggaagcgatc gggctcgatg
      781 agctcatgcg ggagggcgat cctttccgcg cgccgccctt gcgtccacca gcgacgcgac
      841 cagttccgct cggagacaag ccgccggtgc cgagcccgga cgctatcgac gcggccgaca
      901 cacctcccgc gcccgacgcg gctcccatcg acgacgcaag tccggcaacg gatacgccgt
      961 cgagcggtga acccgggtcc gcagcgaact cagaagcgcc cgacgaggca aagggtccat
     1021 gacttccgca gagatccagg tccctgaacc cgaaccgaga gcggtccccg aagaagatca
     1081 ggagatgacc ttcttccagc acatcgcgga gctgcggaca cgcttgcttc gctcgatcct
     1141 cggtctgatt ccagcggtcg cgatcagttg ggtcttcaaa aaggagatca aagacttcct
     1201 cgtcgcaccg ctccaacccg cttgggagaa gctcaatctg cccggtcagg ccgtgctgcg
     1261 attttccggt cctgcggacg ccttcatgat gtacatgcag aactcgctca tcgctggcgt
     1321 gctgctcgcg tcgccttgga tattctggca gctctggatg ttcatcgccc cggggctcta
     1381 caagaaggag aaagcgctcg caattccgtt catcgtcgca tcgacgatct gctttgtcgg
     1441 cggtgcggtc ttcgggtaca agctcgtgtt ccccgaagcg ttcaccgtac ttctcggctt
     1501 cagtgacgag accctcgtcg ccacgatgtt cgtgaaggac tacatgccct tctttcggcg
     1561 catgctgctg gcgtttggcg tcgttttcga ggttccagtc ctcgtcacct ttcttgcggg
     1621 tgcgggcatc gtgacctggc agcagctcat caagttctcg cgatggtgga tcgtctgtgc
     1681 ggcgttcctc gcgatgatcc tcacacccgc ggacgtctac agcatggtga tgatgctcat
     1741 cccgctcgtg ctgctctatt acatcggcgt cgggtgcgcc tacttgatca gcgtattccg
     1801 gaagaagccg agcccttagc cgcgttcggg aaccgggtac ggcggatact tcggcagatc
     1861 ctctggaaga aaccactggt cgccccaaat actggcgcca ccgtcgatgt aaaacgtctg
     1921 gccggcgacg aaatctgcat atctcgatgc gagatatgta atcaagtgag aaacctcctc
     1981 cgccgtcccg aggcgcttca gtgggtttgc cttctctgcg gtctcgagga gctccggcgg
     2041 gtactgcttg gtgccgctgg ttacgatcac gcctggggcg acggcattca cccgaatacc
     2101 gtgtagcgcc cattcgacgg caagagtctt ggtcatgttg tctacgcctg cacgtgccgc
     2161 gccggtgtgc accattccgg ggaagccgcg gatgatctgc gcgatcacgt tcacgattcg
     2221 tccccgtttc tgcggaatca tcgccttggt cgccacggca tgggtcatgc tccaggtacc
     2281 gaccaggttg tttctgatca ccgccgcgaa gccttttggc gatagcgctt cggcggtcgt
     2341 cgggaactgg ccacctgcgt tgttgatcag cacgtcgatg cgcgcaaatc gctcgagcac
     2401 ggcgtccacg tatgcagtga ttgcctccgg ctcgcgaatg tcgcagggag cgccgaaggc
     2461 gtcgatgccc cttgactgga gctcggtgac tgcgttccgc aagggctctg gccgcctgcc
     2521 gcagatcgct atcttcgcac caagcaggcc catctcggtg gcagcgacga ggccgatgcc
     2581 agtgccgcca cccgtgatca atacgacttc gtctgcgaac agcccgtcgg catacacctg
     2641 atccatgacg cggctcatac cacgcagggc gagatccgac gattcgatcc tcagctggcc
     2701 gctacggctt cgctctgtgt cacgagtgtc gccaccagct cccgcaagtc ctgcgcgaag
     2761 aaaggcttgg cgagggtggc ctccacgttc aggcggcgcg cgatgtcgtt tcgcgcagtg
     2821 ctcgcgctca ataggatgac cggcacctcg gagtgcctcc aacggcgatg aaactctctc
     2881 aaaaacatct cgccgtcttc gatgggcagc atcaaatcga cgaccatcag atctggcaga
     2941 gcaaccttgc actgcgcaag tgccgaacgg acgtcaccgg cagcgacgac cgcatgccca
     3001 tcctgctcca gcaggacctg gagcatgtgc cgcaccgagg ggtcgtcatc gagaacgaga
     3061 atcgtcttct tcgcggtcac ccctgcatcc taacaggcgg accgctcatc gcgaatatgc
     3121 gcttcatgcc tacatgagag ctgctatcct cacctgccat gacttcagct ttgcgtgcca
     3181 gcgtgatgtt ttcctgtctg ctggtcgcct gcgcgggcag cgaaccccgg ccttcgtcgc
     3241 tggactcgat cgagctcagc gagttttttc ctctgcacgc aggcaacgct tggtcctacg
     3301 acgtcgacac aggcgagccg tcaaccacgc ttggcgtcac ccgggtcgaa gcgttcgacg
     3361 ggcacctcgc ggaggttcga acgggacgag cgatggtgcg ctacgagctg ctagccgagg
     3421 ggattcgcgt gcccccagag gatgtgtggc ttctccgagc acccctagaa gaaggtgcaa
     3481 gctggcccgc gcgcggaggt cggaccgccc aaatcatatc gatcgacact cgcatcgaaa
     3541 cgctggcagg caaattcgag cgttgcatcg aagtctcgga gaccgggggc aagctcgagc
     3601 tcgagattcg aatcgtctac tgccctggcg taggcccagt cgcgctcgac tccaccatgc
     3661 gttccgagac gagtgatcgg gtcgtaacgg tatttgcgag gcttcgcggt tatgaagtca
     3721 gtcgcgagtc tcgctcgggg ccttagcgga gacatcgacc tcgacgaagt gcgttcggag
     3781 ctggaccact agcgagagcg aaccgtcgac gaggaaaacc tggcgccagc ttcggccgtc
     3841 gccgtctgtc acttcgagcg cgtggcgcgt tcctggcgaa acaccaaaca aagtattgtc
     3901 gattctccgc ccgtcgagcg tgacctcggc ccccgccggt acgccgagaa gctcgaccgt
     3961 cgccatcctt gcgtcgcctt gtgcgatgct gccgtcccct ccgattggtt cgcgttcgat
     4021 caacgcccat gcgatggcca cgagcacggc cagcactgcg aacgccatca tccagcggct
     4081 gtcgagcacc cgccctacgg tctgtaaagg tctgccttcg tcgtcgttgc cggggtaggc
     4141 catctcagct ccgcatcgcc acggtttccg aaacgtgcac gatcgcatcg ctattgtagg
     4201 atgctctcgg aggttccgtc accttcccgg gtggattcac catgacgaaa gcaattctgg
     4261 aagcgcgagg tctcaccaag gcctacggga accgcgtggt cgtggatcac ctcgacctca
     4321 gatgccgccg cggcacggtg ctcgggctcc tcggcccgaa tggggctggc aagaccacga
     4381 ctctgcggat gttgtacggt ttcgtgcctc cggacgaggg ctcgatttcg tacgcaggcc
     4441 agcaattttc ggtcaaccgc gcagaagcca agcgcatgat cggcgtttgc actcaagagg
     4501 actcgctcga ctacgacttc accgtggccg agaacctctt catttacgcg agctacttcc
     4561 gtcctcgggt cgagggtctc gagcgccgcg tcaccgacct cctcgaccgg ttcgagctga
     4621 caccttacgc gaacgtgtcg ccgcatgtgc tctcgggtgg cttcatgagg cggctgctca
     4681 tcgcccgttc gatcgtgcac cgcccgagcg ttctgttcct ggatgaaccg acgacgggcc
     4741 tcgatcccag cgcacgcctc gacgtctggg agctcatcga cgggatgcgc gccgaaggct
     4801 tggcgatcgt gctcacgact cactacatgg acgaggccga gcggctgagc gacgagctgc
     4861 tggttattca tgaagggcag aacgtggcgc acggagagcc gcaccgagtc atcgacagcg
     4921 tcctcggtga ccatgtgatc atcgttcccc gcagcgagcc cgcacgcgag gagatcgcaa
     4981 cttggctccg tgaccgcggt gccgagcagg tcgccaccgt tctcggcgag ctacgggtgc
     5041 cagtgagcaa ggacgacctc gctgagctca gcgcacgctt cactggtgca acctggatgg
     5101 ttcgtccccc caacctcgac gacctcttca ttgcactggc ccgccgcaaa gcgaggcact
     5161 cgtgagcggg ttgcgaaacc tcgccgccgt gtcggattgg cgctcctggg cggtttttcg
     5221 ccggaatacc ctcgtattct tgcgcaattg gcgcacagct ctgattcccc cggcgatgga
     5281 gcccgtcatc ttcttcgttg ccttcgggct cggttttggg ggcttcatcg aggacctcaa
     5341 ctaccatggc gtcgagatca actatgcgac ctacgtttcg gctgggttga tcgcctacac
     5401 ggggtttgga actccgtact atgaagccct ttactctgcc tacgtgcgga tgttctatca
     5461 gaagacctgg gacggtctcc tcgctacgca agtcgagctt ccaaatgtcg tctggggcga
     5521 gatcctctgg ggggcgactc gtggaatggt gaacatctcg atcgtgtcgt cggtcgtcgc
     5581 agtcttcgat cttctgggat tcgtcgaagt gaaggtagag ttcttgcttc tgatgcccct
     5641 tctcggtttc gttgcgggct cggcattcgc atcgtttgct ctcatcttca cggcaatcgt
     5701 tccggcgatc gatcacatga actatccggt gttcctcatc ggctggccgc tcagcgtggt
     5761 gagcaacacc ttctttccga tcgagcccca gagtcagtgg ctcttcgcgc tcatgcaact
     5821 caatccggtc taccaactcg cggaatgcgc gcgagggctc ttggtgctcg gaagcccggg
     5881 ttggcatctc ctcggactgc tcggttcgac ggcgcttttt ctcattccct gcaccctgat
     5941 ttccatccga ttgacccgtc ggcgagtcct cggcgattga gctccgctcg tccataaatt
     6001 cttttgtcag atcaaagagt tagacgtcgc atcagtcctt gcgtcagccc cccttcaagg
     6061 ctagaagtgc cctatggcag atcccccgat cgcggaacag cgacctgctg cacacggcta
     6121 cgatcaagac gcgattcagg tgctcgaagg gctcgaggcc gttcggaagc gtccgggcat
     6181 gtacatcggc gatccgcacg acggatccgg cctacaccac ctcgtctggg aagcggtaga
     6241 caacgcggtc gacgagcatt tggcgggcca ctgcgacgcc gtacaagtca tcgttcactc
     6301 cgagggctcg gtcactgtga tcgacaatgg aagaggtatt cccgtcggga tgcactccaa
     6361 gggtgtgagc gccgccgagg tcgtcatgac ccaactccat gcgggcggga agttcaacag
     6421 cgatagctac tcggtgtccg ctggtttgca cggcgtcggc gtgagcgccg tgaatgcggt
     6481 cagcgaatgg ctcaagatgg agattcgcag ggaaggcaag ctctggtttc aggaatacga
     6541 acgtggcgtg cccaaggcgc cgctgcgcgc gattggaacg agccaaaaga cgggaaccaa
     6601 ggtcacgttc aagcccgacc cccagatctt caccatgacc gacttcagct tcgaggtgct
     6661 gaacaaccgc ctgcgggaga tatcgttcct caatgccggt ctcgaggtct cgcttgaaga
     6721 cgagcgaggt gacggcaaga aggtcatgca caagttcgaa ggcggaattc gagagttcgt
     6781 cgaagcgctc aacaagaaga agacaccgct acacgatgac gtgatttacc tccacgccgt
     6841 caaggacggg gtcgacgtcg agattgcgat gcagtggagc gactcgtaca acgagtcgat
     6901 tttcccgtac acgaacaacg tcttcaatcg cgacggcgga acccatctca ccggccttcg
     6961 caccgcactc actcgcgtca tcaatcagta tgggtcgcgt gagaagcttc taaaagatct
     7021 caaaaacccc ctcagtggcg aggacgtgcg cgagggactg accgcgatca tttcgatcaa
     7081 gcatccggac ccggccttct cgtcccagac gaaggacaag ctcgtctcga gtgaagtcaa
     7141 gggcatcgtc gagagcatcc tgaacgaaaa gctcggtcag tacctcgaag aggaccccaa
     7201 ggcgggtaag cgcatcgtcg agaagacggt cctcgccgcg cgtgccagag aggcggcccg
     7261 ccgggcacgc gagatggtgc agcgcaaggg agtcctcgat gcgagcacgc ttcctggcaa
     7321 gcttgccgac tgtcagagca aagacgcgac cgagagcgaa atctacatcg tcgagggtga
     7381 ttctgcgggc ggaagtgcga agcaaggacg agaccgccga tttcaagcga ttcttccatt
     7441 gcgcgggaag atcctgaacg tcgagcgcgc gcgtttcgag aagatgatct ccagtgcgga
     7501 gatcggtacg ctgatcacgg ctcttgggtg cggcgtagcg ggtggcgaaa acttcgacat
     7561 cgaaaagcta cgctaccacc aggtcatcat catgacggac gcggatgtcg acggttccca
     7621 catccgaacc ctgctgctga ctttcttcta ccgccagatg ccacagctca tcgacaaggg
     7681 atacctctac atcgcgcagc cgccactcta caaagtgaag aagggcaaga agctccgtta
     7741 tctgcaacac gacgacgcct tgagccgcca cctgatcgag ctcggaaccg atggcattca
     7801 gctcagcatg aaagggggcg gcgcggagct cacgggggag ccgcttcgaa atctggtcaa
     7861 agacatggcg cggttccggc tcttgctcga taacatcggg cgtcgttttg acgcgctggt
     7921 agtcgaagcg ctggtcacgg cgaacgatct cgaggtcgag gacctcgcgg atcgcgagaa
     7981 ggtcgaagct ggcatcgagg cgctcaagaa gtacgtcgag tccaagcggc caggcgagat
     8041 cgacacgacc atccaatacg acgaagagca tgatcgccac agggtgatca tcgagaccaa
     8101 ggacagcgga gcgcgtcgcg tgacgaccgt cgatttcgat ttcctttcgg ccggcaacat
     8161 cgcggagctt cggcagatct atcaggggat tcgtgctctc ggagagccgc cgttcgtgct
     8221 cacggtgctc gacaaagacg ggcggcccac gggtgagcca tccgaggttc ccgacctgga
     8281 gtccctctgg gacaccatcg acgccagagc acgtaagggt ctcggtattc agcgctacaa
     8341 aggcctcggc gagatgaacc cggaagagct ctgggagacc acgatggacc cggagacgcg
     8401 gacgctgctt caggttcgca tcgaggacgc gctcgaggct gaggagcttt tcagcgtgct
     8461 catgggtgac caagtggagc cacgcagagc gttcatcgag agcaacgcac tcaacgtgaa
     8521 gaacctggat atctagctgg cgcggcgcga gcggcgcagt agggcttgga ccgcttcatc
     8581 aacccgcggg acttcctgat cgacgaggcc gagcagctgc ctgacggcgt cgacgctcgt
     8641 ttcgcgttcg attgcgccga ggagctctcc caccttggtt gcgccgatct gcttgctcgc
     8701 gcccttgatc gagtgcgcga ttagcccaac ggtgtcgagg tcctgcgctt cgagggcgtc
     8761 gcctagcttc agcatggact ccttgagctg gacgacgtag cccatcatga cctcctcgat
     8821 gaagtcatcg tcctctccgt cgagggagac caggcgtcgg accatctcga ggtcgagcgc
     8881 cgacggtgga gggagggacg acaccggcct cgccgagggc ctaccggaga ccggaaccca
     8941 gcgctccaaa gctctcgcga gcatcgagat gcggaccggt ttgccaatcg tgtcgttcat
     9001 acccgccttc aggcaccgga tccggtcccc ttcgctaacg ctggcggtga gcgcgatgat
     9061 gggcagtcct tcacgaaccc ccgacttccg aatgcgttcc gtcgcttgga atccgtccat
     9121 gttcggcatg cggcaatcca tgaacaccac gtcgtatggg ttgctctttg cctttgccaa
     9181 cgcctctacg ccatccacgg cgatgtccac ttcacaagag agcttcttca ggagcgcctc
     9241 ggtcaccatg cggctggtcg ggttgtcctc cgcgacgagc gcacgcatgc cttcgcgaat
     9301 cagcacttcg cccgcgccgc ccgggtcggc cacgcgaagc gtatcctccc gaccgggcgc
     9361 tagatcgagc tccacccaga acagcgcgcc ctggtcgagt gagctctgca ctccgacttc
     9421 cccgcccatc aagtcgacga gctgcttggt gatcgcgagt ccaagcccgt tcccaacagc
     9481 actgctcttc gggctcgtct cgttccggtg atagcgcgtg aagatccgcg atagggactc
     9541 ctcggggatg ccgatgccgg tgtcccttac ggagaggcgc acacgcgtcg tttcggagcg
     9601 ccggacaacc ttcgcactga cgaggaccga gcccacatcg gtgaacttga tcgcgttgtt
     9661 gacgaaattg aggaggacct gccggatgcg cccggcgtcg ccaacgaaac ggcgatcgct
     9721 gaatcctctt agatctgcat cgaggctgag ccccttcgcc tcagcgcgag gtcgcatgag
     9781 ggagatcaca ccatcgacga cagcagtcag atcgaacgga acctggtcga ggacgacttg
     9841 tcccttctga agctgcgaaa acgtcagcac ttcgtcgacg atcccgacca gagcatccgc
     9901 cgactcgcga atcgccatga tcatcttggt ctgctggtca tcgagattcg tgtcgagcag
     9961 caatgcgctc aggccgagca ccccattcat cggcgtgcgg atctcgtggc tcaagttggc
    10021 catgaactcg gtcttggcct cagaggcgcg ctccgcggcc aagcggcgct cctcgaccgt
    10081 aaccagggtt tggcgacgta cgtggttcag cgcgagagct accaccgaga acccagcgag
    10141 gtatccgatg ctgcgccccc agtccacgcc ttcgaccgaa agcgaggtca aaccccagcc
    10201 gaggtcgccg aggatcatga tcgcgatcac ccacctccgg ctgtgaatca cggcccccgc
    10261 gatgacctga attagtacgt acagatagaa gtcggagggc tcgccgctca gcccgtagca
    10321 gatcgttacc gtcgaggtga ccaccaggaa caaagctccg ccgatgtgca ctgaccacat
    10381 cctgggcggc gcttgacgct tgaagcgcca gctcagggcg agcatcgcca ctccggtgag
    10441 tccgtagatc gtcatcagct tgaggcgcgt ttggtcgacg tcgctcgacc actggccgaa
    10501 ggagagtccg aggtacgcga ggcctgcgct gaggaacatg gtcaccacgt gccgcgcggt
    10561 cgtcgcgttg aacatagccc agcgctggcc ggcatcgagt ggctcgtgtg cggcgacggc
    10621 ttcagtggaa ggagcgaatc cctgctccca agcgctttct tccttctgcc cgacccccat
    10681 ccaaaccagt gtatcggccg ccgggccgcg attcacggct tgtggggctg aagtttgtgt
    10741 cgtgcctcac gaatcgtgcg aaccgccgct tcgtggagga gtccgttcga cgcgatcaat
    10801 tttcccgtgc gcggatcggc ggcgtctccc tcgtaggtcg agaggcgtcc gccggcctcc
    10861 tgcacgatca gcgcaccggc gcacatgtcc caagcgttga gactctgctc ccaatagagg
    10921 tcatacgtgc cgtcggcgac caggcagagg tcgatcgccg ctgagccgca gcggcggatg
    10981 ccccttgctc gctgcagaaa gagggccaac tcggcatgat tgttgtctgg gttcgaccac
    11041 cgatcatagg ggaatcccgt tgcgcagagg gcttcttcga gccgagcacg cttcgacacc
    11101 gaacaggggc tgtcgttgcg aaaggcgccg cggcctctgg tggctttcca ggtgacgtcg
    11161 agggcgggtg catgcaccac gccgatcagc ccctcggaac cgcgatagag cgcgatggac
    11221 atcgcgaaga agaaatggcc gtgggcgaag ttcgtcgttc catcgattgg gtcgacgtac
    11281 cagaccatct ctccttctcc ggtctcttcg ccttcctcgc cgacgatccg gtgacttggg
    11341 aacgccgagg agagctcctt gcgaatcaga cgctcgctct cgaggtcgta ctcggtgaga
    11401 aggtcaaagc gacccttttt cgagacctcg gacaccgagc gccaggctcg catcacgagc
    11461 ccgccagctt cccgggcggc tcgctcggcc acctcgagcg cgcgctcgct atcctcttcg
    11521 ttaggcatga agtcttgcat tttcgcggat ttccgggact gcgctatgcg taactgagcg
    11581 cctgaagggt gttcgctggc atttccgccc caaagcccgt aaaaggcggt gcgctcctga
    11641 gcccgcgttt cgagcgttta ccaatgtagg ccaatgtagt tatgcccgac aaggacaccg
    11701 tcacgccgag cattcgcaac ccattcaaag tggatcgcga tcccagtctc ccaagcgaca
    11761 aacccgaggc gcaacggccc tacgacgagg ccgtacgaga aggtgagcag ctgcgcgcgc
    11821 gcccgcgcaa ggcggcgggg ccagttcaga ttggcaagca gcacgacaag cgccgcatga
    11881 ccgtctggga gaggatggat gttctccggg atccgggaac cgagccgacg attctctatc
    11941 agaactgggg gccgaacctc gatggggcgt cgattgtcac ggccgtcatc gaggtcgaag
    12001 gccgtgaggt tgcgctctac ggtcatgact tcacggttcg cgccggttcg atggatgcaa
    12061 ccaacggcac caagctcgcc aatctcattc gactggccgg tcggcaaggg atgccgctcg
    12121 tcggaatgaa tgacagtgca ggagctttcg ttcctgcagg aatcggtgga ctcgatggct
    12181 acgcggaggc gttcacggca ctgcggaaga tcagtggcgt ggtcccgagc atcatgtgca
    12241 tgtttggcta caacgccggc ggcggtgcat acctgcctcg gcagggaagc ttcatgatcc
    12301 agccttccaa cacgtttttc ggcctcaccg gccccggggt cgtgaagtcg gttctcgggg
    12361 aagacgtgac cgccgatgag ctcggcggac cgggggtcca cggacagagt ggcgtggtcg
    12421 atctcaccgt acgggacgag gttggcgctc ttagaaccgc tcggcgcttg ctggcttatc
    12481 tgcccggcaa caatcacgaa ttcgccccat atcaggagac ttccgacccg atcgaccgtc
    12541 ccacaggcga gatcgacacg ctcctgcgaa aggcgttcaa ctcgccgacc gggttcaaca
    12601 cgcccatcga cgtcagcatc atcctgcagc aaatctgcga ccacggtgat tacttcgagc
    12661 tccagcccca gagggcccga aacacgatca ccgcgttcgg ccgcctgggc ggacacgtgg
    12721 tcggtttcgt ttgcaacaac agcgcggtcg cttcgggtca aatcgatgtc gatgccgcat
    12781 tcaagaactc gcggttcatt cgattttgca acctctacaa cattccggtc atcttcatgg
    12841 aggacaccac gggtttcttg ccgggtcgtg accaggaggg ccgcgggatc gtgcaggcgg
    12901 gtcgcgcgat gctggattcg atcatcgatc tgcggacacc gcgtctcttg ttgatcattc
    12961 ggaatgcgtt tggcggcgcc tatgcggcct tcaactgcta tgcgaccggt gccgaccacg
    13021 tgatggcgct tccgactacg cgcgtcgcgg tgatgggacc tgcgggcaag gagttcgtgt
    13081 acaaacaaga gatgcgcgac acgcgaacca aggcgaagct ccgcgtgcag gaagccattg
    13141 cggccggtgc gtccaagaac gacgccgagg ccgaagccgc ggcctggctc aaggagcagg
    13201 acgcagcatt caacgcgcga tatgaaaggg agctcatgaa tcccaaggaa gcgctgagcc
    13261 tcggttcgat ttccgaaatc gtcatgccga ccgacgtccg agccgcgctg gcgaaaaacc
    13321 tcaacttcta tctctcccac tacacaccgg agccgcttca ggcggtgcag cgtgagttcc
    13381 attgatggat tccgacaact acttgcacaa cccgctcatt catcgggatc ggcgccttgg
    13441 cgagtccgaa tccgaatgga ctcgctcctt cgcctgcgaa gacatgaccg tgctcatcgt
    13501 ctgccgcggc cccgtgcggc aggaagcgct ggacaccttc agcgaaatgg gaatgacgca
    13561 cgtcgggatc cttctctccg agaaagattc gatcgtctac ccgcgggccc tggccccgga
    13621 gctccgccga ctcgacccat cacgcgtgca cgcggtatcc gactacacgg gtgccagtaa
    13681 ggaagagcga acgcagcgca tcgctcagat catcaagatc gcaaagaaga atgggtacga
    13741 ctacatcttc gccggctacg gattcatggc ggaggatgaa gatttcgtcc gctcgatcga
    13801 agaggctggg cttcgtttca tcggtccttg ttcgcacacg gtccaggctg ccggcttcaa
    13861 ggacgaggcg aaacgcacag ccgagcgcgt cgacgtgagc gtgacgccgg gcatcaacaa
    13921 cgctacggct aggctccttc tccggaagca cccggatctg aaggcactga gcaagctggc
    13981 cgagaaacac tcgctcgaag tcgccgagct cagcgatgga gcgctcgagc tcgagaccgt
    14041 cgccgatggc gtcttggcag cgtcctacga tgctggaatc gatctctaca ccgtcgacga
    14101 gctcggcgaa cagatcgagc tcgaggtcgc gtcgattctg aagaagtacg aggggagccg
    14161 gattcgcctg aaggcgattg gtggcggtgg aggcaaaggg cagcgcatcg taaacgccgc
    14221 aaagcaagcg ccagcgatgg tgcgcgagat cttgggcgag gtgaaggcgc tcggcgtcgg
    14281 cgacaacaag aacatcctcg tcgagctcaa catcgagacc acgcgccaca acgagatcca
    14341 gcttcttggg aacggcgaat ggaccgtttc cctcggcggc cgcgattgct cgctccagat
    14401 gcacgagcag aagctgctcg aggtgtcgat cacccaggaa acattagctg ccgcggtcga
    14461 gcgagagcgc gctgcgggga acgaggctca cgccgctgcg atcgagcaag acctcgacat
    14521 tctgcgccga atggaggcag aggccgagcg ctttggtgaa gcggtgaagc tcgattcggc
    14581 atcgaccttc gagtgcatcg tcgacggtgc gcgccattac ttcatggaag tcaacacgcg
    14641 cattcaggtc gaacatcgtg tctccgagct ctgctatgcg cttcgatttc gaaatcccga
    14701 ggatcgggac gagttcttcg acatccactc gctcgtcgag gcgatggctt tgctcgcgaa
    14761 gcacaaggtc aggcttcccc ggcccgagcg cgtgcagcgc catgcagccg ccgtagaggc
    14821 tcgtctgaac gccacggacc agtccttgtc cccgcatgcg ggcggagaga tcatgtcctg
    14881 gtccgacccg atcgaagggg aagtgcgcga tgaccagggg atttgcttca agaaccccga
    14941 cacgcatctc ttcatgcgct acaagctcgc cggcgcatac gacagcaaca tcgcgctcct
    15001 ggtcacgacg ggtgaggacc gccgagatag ctacgagcgc ttgcgcgagg tgctccggcg
    15061 aacggtgatt cgcggcccag atctgcagac gaatctgcag tttcactacg gcctggtgca
    15121 ttggttcttg gcgaacggtg tcgatgccaa gccgacgacc cgctttgtgg tgccttacct
    15181 cactctcgca ggggagcttc tccacgagtc caacgaaatc gacgtggact acgcctatga
    15241 ccggctctgc cgcaagtatc tgtccgccgt ctcgaccgac cccgcggcag ccaaagctac
    15301 gaccgacctt tcggtcttga agaagacgct tctcgggcgg ccgctcggca ggattctcag
    15361 gcagccgcat ctcttgtcgg cctggctcag tgcgaacaag aaccacttcg aagtgaagaa
    15421 aggccgcgtc acttggttgc gcaacccgct ctcggtgctc gcggacacgt attttcttct
    15481 ccacatggac gcggaggtcg acctcccagc cgcgcaccgc atttgggaac aggaccagac
    15541 cgtgctgcgg gacggcgccg ctttctacga agccgtcgag gagcgcttgg gcagccgtcc
    15601 ttggccggag ctcgatcaag cgttgaaagc gaagaagccg cctaagggtt tcgatgccgc
    15661 gctatggcag gaggtgcgtg aagcgcatct cggtttccag ctcggcatgg agctctatag
    15721 cttgttgccg ctgatcgggg acaaggttgg gttcttcgac ctccgagtga acgacgacct
    15781 caccatcagc atcccggagc ggctcctcga ttccgcccac caggcgaaga tgaagaaggt
    15841 gctcgtaccg ccgccggtca acaaggccga tgagatcgtg gctgtgagcg gcgggatgta
    15901 ctatgcgcag gaagcgccgg gcatgcctca tttcatcgaa gaggggcagc acttcgagat
    15961 tggcgatccg ctctacatcg tcgaagtcat gaagatgttc aacaaggtct acgcgccttt
    16021 cgctggcacc gtcgacaaga tcctcgtcga gacgaatgga actatcgttt ccaagggcca
    16081 ggcgctgttc aaggtcactc ccgatgaaat tttcgtcgaa gaagatccag tcgaagttcg
    16141 agcacggatc caatcgaaca ccgagcagta cctcagcgtt gtcgcctgag gctccgagcc
    16201 gtttagcggc ggtcgtcgcg cggggtcgcg agaacgactc ggttgcgtcc ggcccctttc
    16261 gcgcggaata gcgcgcgatc tgccgtggtc atcagccgcg acttcagatc actcggtggt
    16321 tgaccgttga ggtcgcgaag gcctagcaga gtggcgacgc ccacggacac cgtgatcttg
    16381 ggaagtgtgc cgcctgggcg catcgcgcct ccagtgcttg cagccacgtg gctgcgcata
    16441 tgatccgcga gggcccgcgc gctttccttc gaggcgcgct tttgcacgac cacgaactcg
    16501 tcacctccga agcgaaagac ctcgccgacg ccagctgcag cctcttccag agccttgccc
    16561 gttctcttga gcgcctcgct tcctgccgca tgcccgagct tgctgttcac ccgcttcagg
    16621 tggtcgacat cgacgaacat gatcgccatg tcgcgctgac cgcggcgtgc cgaggacacg
    16681 gctttggtga gctcgttgtt gagctcccgc acgttcctga ggccggtgag tgggtcgtgc
    16741 gcggcgagcc cctcttgccg tcgaagcgag gaggtctgga tcaaagatgt ggtgatgtaa
    16801 cgcgacagac cttcgacgat gcgcgtttcc cgctcggtga aacgaaaggg cggctccgga
    16861 tcggtcaact gcagaaaacc gaatgggcgg cctccatcgt gcagtgaagc gagtagcgca
    16921 gtccaagtct cgtctgcagc atgaatcgag agctctgaat cctctcgaga cgaaggaacc
    16981 acgaagggtg tccggcgtcc gagcgctcgg gcggcgaggt ccggatgagg cggaccgcct
    17041 cgttcgagca aggacttcgc atcgggcccg tgcgagacca cgcagcgaag gcagtctacg
    17101 ttctggccga tgtagatcgc cccggccgtc gcgcgaaccg cgagcactga cgcctctagc
    17161 gcttcttcga gcacacgcgt gacggaccgc gcccgggagc tcgacgcccc gagcacggat
    17221 tcctcgagct ttggccccct caaggtgtga atccgcacac tcaaatccta ggcagttcca
    17281 gactctcgag ccaacttggt atgctcctgt ggtggcctgt aggcactgct ggcttggcgt
    17341 ggcgaaacgc atgatcgtct cccgcgccaa gttctacgga tggcggttcg gccgggttac
    17401 actatgaaac tcagggggca ccgttgccga acaaggacga tcttcttcga aaataccgag
    17461 tgatgatgct gattcggcgc ttcgaggaag aggcggcgcg tcactatgca aaggggaata
    17521 tcggaggatt tctccacctt tacatcggcc aagaggcgat cgcagtcgct gcggctgacg
    17581 tcctccagcc ccaggactac atctttcaga cctaccgtga tcacggcatc gcgctggccc
    17641 gcggaatgtc acccaactcc gccatggcgg agctttttgg caaggatacg ggctgttcgc
    17701 ggggcttcgg gggctcgatg cacttcttcg acgtcgagcg tggcttcctc ggcggctggg
    17761 cgatcgtggg cggtcacctc gccttggcgg ccggtacggc cttcaagtcg aagtacaacg
    17821 gagaagaccg agtcaccatc tgtttcttcg gcgagggctc gacgaacata ggcggcttcc
    17881 atgaagcgat gagcctggcc ggtctctgga agctgccaat cgtcttcgtc tgcgagaaca
    17941 atcagtacgc gatggggacg ccgctcgaac ggaccagccc gctcagggat ctctcgatca
    18001 aggccgaggg ctacggcatg atgggcgacc acttggaggg gcacgatgtg gatgtcgtcc
    18061 aagcgcacct cgccaaggcc gtcgatcgcg cacgaactgg gggcggaccg actttcgtcg
    18121 agatcaagac gtatcggttt cgtgggcact cgatgagcga cccggcgaag taccgaactc
    18181 gcaccgagct cgagcagcgc aaacagcaag accctgtcac cgtggttcgt gagaggctcc
    18241 tcggcatggg cgccgatgag acggtgctcg atgacatcga tctcgaagtc gacgaaacac
    18301 tcgacgcggc cgtgcgtttc gcagaagaat cagagccggc tcgtgaggaa ctcatgatga
    18361 gcacggcgct cgctccggaa ggtgattcgt gagggagctg cgttacagag aagctctccg
    18421 cgaagcgatg ctcgaggaga tggaacggga cgagcgcgtc ttcttgatgg gtgaggaagt
    18481 cgggcactat cagggggcgt acaaggtctc ggagggcttg ctcgagaagt tcggtgagaa
    18541 acgcgtcatc gacacgccaa ttgcggaggc cggtttcgcg ggcgttggca tcggcgccgc
    18601 gatggtcggc cttcgcccga tcatcgagtt catgacctgg aatttctcct tcgtcgcgtt
    18661 cgaccagatc atcagcaacg cagccaaagt tcaccagatg agcgcgggcg cgattcgcgt
    18721 acccatcgtg tttcgcggac ccaacggtgc ggcgaaacaa gtcggctctc aacacagcca
    18781 cagctgcgcg ccgttctaca cgagcattcc cgggctctac gtgatccagc cgagcacgcc
    18841 ccgtgacgcg aaggggctcc tcaagagcgc gattcgaagt aacaaccccg tgatctttct
    18901 cgaaggggaa acgctgtacg gcgtcaaagg tgaggtcccg gacgaaggcg tcgaggagct
    18961 gatcccgatc ggagaaagcc gcctggcgcg gcccggtgcg gattgcacga tcgtgagctg
    19021 gggccgccct tactacaccg tgctcggagc ggtcgagaag ctcgcggccg agggcatcga
    19081 ctgcgaggtc atcgacccga ggacgcttcg tccgctcgac accaagctga tggtggagag
    19141 cgtgaaaaag accaatcgct gcgtcgtggt tcacgagcac tggccctacg gcggcccggg
    19201 tgccgagatc gtcgaccgga ttcaacggga ggcgttcgac tatctcgacg cgcccgtaga
    19261 gcgggtgacc gggctcgacg tgccgatgcc ctacgccctg aacctggaag ataaagtcct
    19321 gccaaccgag gagcgagtga tcgacgcggt caagcgcgtc agttacgtca agtgagtcat
    19381 ggctaagatc atcggactac ccaagctttc tccgaccatg gaagaaggca ccctcgtcgc
    19441 ctggatcaag aaggagggcg atgaggtgga ggtcgatgac cttctcgcgg aggtcgaaac
    19501 cgacaaggcc acgatggagt tccgctcctt cgaccgcggc gtcttgctca aaatcctcgt
    19561 gaaggaaggc gagaccctgg agccggatgt gcccgtggcg atcatcggga aggccggtga
    19621 ggacattggg gagctgcttg cgcaggtgga ggcccgggca gggacagcgg cagggacagc
    19681 ggcagagcca gcggcagcgc cagagtcagc ggcagagtca gcggcagtgt cggagccggc
    19741 ggcagtgtcg gagccggcgg cggtgtcggc gccagcggcg gtggtcgaga tggagtcttc
    19801 cgatcgaatt ctctcttcgc ctctcgttcg tcgacttgct cgcgaaaaga gcatcgatct
    19861 tcgtgcggtc gaagggtcgg ggccgcgggg ccggatcatc aaacgagata tcgactccta
    19921 cgaagaaagg ccgagctccg cacgaacgta tagtggcgag cgtctcccgc cgcgcgtgct
    19981 gcaagccagc agcatgcggc ggacgattgc gcggcagctc accg
//
LOCUS       DASRZF010000173         2132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_807075, whole genome shotgun sequence.
ACCESSION   DASRZF010000173 DASRZF010000000
VERSION     DASRZF010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_807075"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            281..1156
                     /locus_tag="VFG22_07850"
     CDS             281..1156
                     /locus_tag="VFG22_07850"
                     /inference="COORDINATES: protein motif:HMM:NF024096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HET6416196.1"
                     /translation="MSAATKVEPFVPTEGMHLDLPMEVYRAAEGVAASDLKNMQRSPA
                     FARMRSHVTSPSKEWGTAVHTAVLEPHALEDRYGLDPESPKGGYPAGWRNTKDYKAQR
                     AELFETQPTLEGLLTPQEFQDLGRIQTRVRESEVGEMIEGVRGEREASVWHYDDEFSL
                     WRKVRPDYLISAARMVVDLKTAVDHRERAFSRACHNYGYHVSDAYYRDTITAALDFDV
                     DFYLFLVVASDAPFEVAGYVLDNDSVKQGRHEYRHQLAKWRECEERQRWPGGIHDITE
                     IRLPEYGINYMNGDI"
     gene            1156..1905
                     /locus_tag="VFG22_07855"
     CDS             1156..1905
                     /locus_tag="VFG22_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416197.1"
                     /translation="MSMTETTENKPAKHADIIPSDQERTFALAQRQAMAFANSDIVPD
                     TFRGNMANCMIALELAGRIGASPLMVAQNLDVIHGRPSFRATFLIACLNRCGRFTPLR
                     YKIEGDGMKKSCIAYAHDTETGELYEGPPVTMQMAHDEGWSTKKGSKWLTMPDLMLRY
                     RAAAFFARTTAPEVAMGLYTADEAWDMAAPKEASTNAKDAAEALRSARDVTPAKDDVE
                     VDEFDEPAQDVEDEFDPETGECIPRDLFAGN"
BASE COUNT          471 a          631 c          676 g          354 t
ORIGIN      
        1 ggaagaggag gaggagtggc aacgctatct caccgagcgg cgcacaagat ccaagcgcga
       61 ctaggcttcc gctactgcct cgcgtgcaac cacctcacgt cgaaccagta ccgccctgtc
      121 agttttgccc tgagcggcaa acggcccgtg tcgtcgagtg acgatgcgac tcctccctag
      181 cgcacagccg cctagtgcag gagaggcgcc cggcccacat cgtggtgctt ctgggaggag
      241 tattgccggg cgccggccac agttttagag gagactcacc atgtccgcag caaccaaagt
      301 agagccgttc gtcccgaccg aggggatgca cctcgacctg ccgatggagg tctatcgcgc
      361 agccgaaggt gtcgccgcgt cggacctcaa gaacatgcaa cggagcccag ccttcgccag
      421 gatgcggtcg catgtgacga gcccgtcgaa ggaatggggc accgccgtac acaccgccgt
      481 actcgaacct cacgcgctcg aagatcggta tggactcgac cctgagagtc caaaaggagg
      541 ttacccggcg ggttggcgca acacgaagga ctacaaggca cagcgagccg agctcttcga
      601 aacgcagcca acactcgagg gcctgcttac gccgcaggaa ttccaagacc tcggccgaat
      661 ccagacgcga gtgcgcgaat ccgaggtcgg cgagatgatc gagggcgtgc gaggcgagcg
      721 cgaggcgagt gtctggcact atgacgatga gttctcgctg tggcgcaaag tgaggccgga
      781 ctacctgatc tcggcagcac gcatggtcgt cgacctgaaa acggccgtcg atcaccggga
      841 gcgcgcattc tcacgcgcct gccataacta cggctaccac gtaagcgacg cctattaccg
      901 ggacacgatc acggctgcgc tcgacttcga cgttgacttc tacttgtttc tcgtcgtggc
      961 ctcggatgcg ccgttcgagg tcgccggata cgtgctcgac aacgactctg tcaaacaagg
     1021 acggcacgag tatcggcacc aattggctaa gtggagggag tgcgaagagc ggcagcggtg
     1081 gcctggaggc atccacgaca tcaccgagat tcggctgccg gagtacggaa tcaactacat
     1141 gaacggagat atctgatgag catgacagag acgacagaga acaagccagc aaaacacgcc
     1201 gacatcatcc cgagcgatca ggaacgaaca ttcgcactcg ctcagcgtca agcgatggcc
     1261 ttcgcaaata gcgacatcgt gcccgacacg ttccgaggaa acatggcgaa ctgcatgatt
     1321 gcgctcgaac tcgccggacg gatcggcgct tcgccgctca tggtcgcgca gaatctcgac
     1381 gtgattcacg ggcgacccag tttccgggcc acgttcctaa tcgcgtgcct gaatcgctgc
     1441 ggtcggttca cgccgctccg gtacaagatc gaaggcgatg ggatgaagaa gagctgtatt
     1501 gcttacgcgc acgatacgga gacgggtgag ctctacgaag gaccgcccgt gacgatgcag
     1561 atggcccacg atgagggctg gtcgacgaaa aagggcagca agtggctcac gatgcctgac
     1621 ctcatgctgc gctaccgagc tgccgcgttc ttcgcacgca cgactgcgcc cgaggtcgcc
     1681 atgggcctct ataccgccga tgaggcgtgg gatatggccg ctccgaaaga ggccagcacg
     1741 aacgcgaagg acgccgcaga agcgttgcgg agtgcgcgag acgtgacgcc ggcaaaggac
     1801 gatgttgagg tcgacgagtt cgatgagccg gctcaggacg tggaagatga gtttgatcca
     1861 gaaacgggag agtgtatccc gcgcgacctg ttcgcgggta actgatttgc cgcgctcaag
     1921 tcgagcgcgt ctaacggccc tgtcgagcga ctggatttga acgcacggca gactcgccta
     1981 gcagggtagt agctgcgaag cgggtgcccg ggaacctatc agaaccgggc tggcctttga
     2041 tttaggggga gtgatgaccc tataccgcct agttggtgtc gtgccacccg aacaaccctt
     2101 ctgcgactgg cgcgtctgcg actggcgcag gg
//
LOCUS       DASRZF010000174         4064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1838363, whole genome shotgun sequence.
ACCESSION   DASRZF010000174 DASRZF010000000
VERSION     DASRZF010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1838363"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /locus_tag="VFG22_07860"
     CDS             complement(<1..953)
                     /locus_tag="VFG22_07860"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET6416198.1"
                     /translation="MRTFGRMNEPPGFGTNGPPIETELTRDLGLVSVLAIGVGTMIAA
                     GIFTLSGLAVGYVGSAAIASFLLAALVATFTALTYCEFSALYPQSGEGYLYARRTFPA
                     LIAYMVGWCLLLGYTASCGFYIASFSSYFNEFIVHSRIEVLPGLVTVAALTLLNIKGT
                     KESGTFQVVVTIGKVLLLFWFVGGGLFHVDPEVIVERFSTDVGMIGSTAAMVFITFFG
                     FSAIAASAGEVKNPIKTIPRAIFISMGFVTVLYTLVVLVVVAADLTEYSESAMGTAAK
                     DFLGPVGGMVIVAGALFSMVSASNASILAGSRVALSMSRLG"
     gene            1127..1765
                     /locus_tag="VFG22_07865"
     CDS             1127..1765
                     /locus_tag="VFG22_07865"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HET6416199.1"
                     /translation="MSLKLGTTAPDFQAETTEGPIRFHDWAGDSWVVFFSHPADYTPV
                     CTTELGLVAKLKEKFAKRNTKALAISVDSLESHLGWIKDIEDTQNVKMNFPIVADPDQ
                     KVATLYEMIHPKADAKLTVRSVFFIDPKKKIRATITYPPATGRNFEEVLRVLDGLQLT
                     DSYQVATPGNWKDGEDVVIVPSITDAAEIKKKFPKGHKELRPYLRMTPQPNK"
     gene            complement(1842..2201)
                     /locus_tag="VFG22_07870"
     CDS             complement(1842..2201)
                     /locus_tag="VFG22_07870"
                     /inference="COORDINATES: protein motif:HMM:NF039825.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5684 domain-containing protein"
                     /protein_id="HET6416200.1"
                     /translation="MDGNEGSFFGLIIQLVVIAVYAWVAWKIFEKAGKPGWAALIPIY
                     NAIVFLEIVGRPWWWIFLFFIPLVNIVIGIIVALDLSRSFGHDLAFALGLILLGFVFY
                     PILAFGADTYRGPSAAG"
     gene            complement(2262..>4064)
                     /locus_tag="VFG22_07875"
     CDS             complement(2262..>4064)
                     /locus_tag="VFG22_07875"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HET6416201.1"
                     /translation="LKYPDLPLGMTDIQLDAKAVHPGGNLDKMRIDVPKYAITAGKSH
                     ANGRLSVTRPISQPEIELVLDGRFDLAEIAKAYPVPEVEAIEGLVVAKVDLSAKGERI
                     EKLTGEIRVTDLLYRPEKAPAIRIPTAQIELSPESTKIKELRAEVAESDVAMNGIVSP
                     ITVLLLGDQPTSASIWVQSKRLRVEDFLAPDAPADSSEPGGFVLPDDVDARLQFEVGV
                     LTYGDLVLKDFKGSGRIRNRKLVLNDVRANALGGSMKLDGTMATPVDAPAVFDIHYAV
                     DKVRFADAFQALPSLRAYAPIARYLDGRFSTDFNAKGTLDEELSPNLDSIDASGLASA
                     FQTKLSSDFKPLALLNEAIPSIPKPLDMESIRARFQIKDGAIQLKPTTVQARGVSMQL
                     SGTHGLDQEMKYQVTSDVPLDKLTSSLAKEVKVLGVDLSKAKTVAVRAKLTGSIQSPR
                     ISVDVDTSALRGAVADAVSAELAEQKARAFGEAAKQAKRLVDEAEKRADQIRAEAAKA
                     AEQLRKEGYARADQVQKEGAGNPLKEIAAKEGAKRIRSETDKRTKQLTAEADKRADQM
                     VAEARKRADQTLQEAVKRGDQATDSIEKQTDKAR"
BASE COUNT          839 a         1267 c         1184 g          774 t
ORIGIN      
        1 tgccccagcc ggctcatcga gagcgcgacg cgcgaacccg ccaggatgga ggcgttcgac
       61 gccgacacca tcgaaaagag cgcgccagcc acgatcacca tgcccccaac cgggccgagg
      121 aagtccttgg ccgccgttcc catcgccgat tcgctgtact cggtgaggtc ggcggccacc
      181 acgacgagca ccaccaaggt gtagaggacg gtgacgaagc ccatcgagat gaagatcgcg
      241 cgaggaatcg tcttgatcgg gttcttgact tcgccagctg aagccgcgat tgcagagaac
      301 ccgaagaaag tgatgaacac catcgctgca gtgctgccga tcatgccgac gtcggtactg
      361 aatcgctcga cgatcacctc cgggtccacg tgaaacaacc cgccgcccac gaaccagaac
      421 aggagcagca ccttgccgat ggtgacgact acctggaagg tcccgctctc cttggtgccc
      481 ttgatgttga gcagcgtcag cgccgcgacc gtgacgagcc caggaagaac ctcgatgcgc
      541 gaatgcacga tgaactcgtt gaagtagctc gagaaactag caatgtagaa gccgcacgag
      601 gccgtgtaac cgagcagcag gcaccagccg accatgtaag cgatcagggc cggaaaggtt
      661 cggcgtgcgt agaggtagcc ctcccccgac tgcgggtaga gcgcgctgaa ctcgcagtag
      721 gtgagcgccg tgaacgtggc gacgagcgcg gcgagcagaa acgatgcgat cgccgctgac
      781 ccgacgtagc cgacggcgag gccggagagc gtgaaaatgc ccgccgcgat catcgtaccc
      841 acgccgatcg cgagcaccga gacgaggccg aggtcgcggg tcagctccgt ttcgattgga
      901 ggaccgttgg ttccgaaacc tggtggctcg ttcatgcggc cgaaggttcg catgccgggg
      961 cccccttgtg gaaccaccta tctcgcgtga gcgatggacc gagaccaggt cgctgaaaaa
     1021 aagagccgac cccacttacg cccacggcgg ggtgccctag cgtctgggag acaagaggga
     1081 tagctttcgg tcccctgcgt cccaaccgac aaacaaagga gccatcatga gcctcaagtt
     1141 aggaaccacc gcccccgatt tccaggccga gaccacagag ggtcccattc gctttcacga
     1201 ctgggccggt gacagctggg tcgtcttctt ctcgcacccg gccgactaca ctccggtctg
     1261 caccaccgag ctcggtctgg tcgccaagct caaagagaag tttgcgaagc gcaacaccaa
     1321 ggccctcgcg atcagcgtcg attcgctcga atcccatctc ggctggatca aggacatcga
     1381 ggacacccaa aacgtcaaga tgaactttcc catcgtcgcc gacccagatc aaaaagtcgc
     1441 caccttgtac gaaatgatcc accccaaagc cgacgccaag ctcacggtgc gctcggtctt
     1501 cttcatcgat ccgaaaaaga agattcgcgc caccatcacc tacccgccgg ccacgggccg
     1561 aaacttcgaa gaggtccttc gcgtcctcga cggactccaa ctcacggaca gctaccaagt
     1621 ggcaacaccc ggaaactgga aggacggcga agatgtcgtg atcgtccctt cgatcaccga
     1681 tgccgctgaa atcaagaaga agttcccgaa gggccacaaa gagctgcgcc cgtatttgag
     1741 aatgacgcct cagcccaaca agtagtcgcc ccgagtgaca ggcattcatg ggcgactgct
     1801 actaaatcga ccagaccgtg gcgtcggtcc gtacgtaccg gtcaacccgc ggcggatgga
     1861 cctcgatagg tgtccgcacc aaacgcgagg attggataga aaacgaagcc cagcaagatg
     1921 aggccgaggg cgaacgcgag gtcgtgtccg aacgaccgcg atagatccag cgccactatg
     1981 attccaatga cgatgttgac caacggaatg aagaacagaa agatccacca ccaggggcgt
     2041 cctacgatct cgagaaagac gatcgcgttg tagatcggga tgagcgccgc ccagccgggc
     2101 ttgccagctt tctcgaagat tttccaggcc acccaggcgt agaccgcgat gactacgagt
     2161 tgaatgatca aaccaaagaa actcccctca tttccatcca tgtctgctct ccttgcagcg
     2221 ccctagggac gcatcaggag actaacacgg gtctccctct ctcaacgagc cttgtcggtc
     2281 tgcttctcga ttgagtccgt tgcctggtcg ccgcgtttga cggcctcttg cagcgtttga
     2341 tccgcccgct tccgcgcctc cgcgaccatt tggtccgcgc gcttgtcggc ttcggcagtc
     2401 agctgcttgg ttcgcttgtc ggtctcgctt cgaatgcgct tggcgccttc cttggcggca
     2461 atttccttga gcgggttccc cgcaccctct ttttgcacct ggtcagcgcg cgcatagcct
     2521 tcttttcgta gctgctccgc agccttggcg gcttcagcac gaatctgatc ggcgcgcttc
     2581 tcggcctcgt cgaccaacct cttggcctgc ttggcggctt ctccgaacgc acgagccttt
     2641 tgctcagcta gctctgcaga gaccgcgtcg gcgactgcac cccgcagagc cgaagtgtcc
     2701 acatccaccg agatgcgggg tgattggatc gacccggtca gcttggcgcg tacagcaacg
     2761 gtcttcgctt tcgagaggtc cactccgagt accttcactt ccttggcgag cgacgatgtg
     2821 agcttgtcca aaggaacatc gctcgtgacc tggtacttca tctcctggtc gaggccgtga
     2881 gtgccactga gctgcatcga gacgccccgc gcttgcacag tcgtgggttt gagctggatc
     2941 gcaccatctt tgatctggaa acgtgcccga atgctttcca tgtcgagggg cttcggaatg
     3001 gacggaatgg cctcgttcaa cagggcaagc ggcttgaagt cgctgctgag cttggtttgg
     3061 aacgcggatg caaggccact ggcatcgatc gaatcgagat tgggcgagag ctcctcgtcc
     3121 agcgtgccct tggcgttgaa gtcggtcgaa aaccggccgt cgagatatcg tgcgatgggc
     3181 gcgtaggcgc gaagagacgg aagcgcctgg aacgcatctg cgaagcggac cttgtcgact
     3241 gcgtagtgga tatcgaatac ggccggagca tcgaccggcg tcgccatagt gccatcgagc
     3301 ttcatcgaac cgccgagggc gtttgcgcgt acgtcgttga gaacgagctt tcgatttcga
     3361 attcgcccag atcctttgaa atccttcaag accaggtcgc cgtatgtcag cacgccgact
     3421 tcgaattgga gcctagcgtc gacgtcgtca ggaagcacga atccgcccgg ctcgctgcta
     3481 tccgcgggag cgtcaggggc caagaagtcc tcgacgcgaa gtcgtttgga ttgcacccaa
     3541 atgctcgcgc tagtcggttg atctcctaga agcagcactg tgatcggcga gacgatcccg
     3601 ttcatcgcta cgtcgctttc agcgacctca gccctgagct ctttgatttt cgtgctctcg
     3661 ggcgaaagct cgatctgcgc cgtgggaatc cggatcgcgg gtgccttttc gggacgatag
     3721 agcagatcgg tgaccctgat ctctcccgtc agcttttcga tccgctcgcc cttcgcgctg
     3781 aggtcgacct tggcgacgac caagccctcg atggcctcca cctcgggcac cggataggcc
     3841 ttggcaatct ccgctagatc gaatcgaccg tcgagcacga gctcgatctc gggttgcgaa
     3901 atcggtcgtg tgacgctcag gcgcccgttc gcgtggctct ttcccgcggt gatggcatac
     3961 ttcggcacgt cgatccgcat cttgtcgagg tttccgccgg ggtgaaccgc tttcgcatcg
     4021 agttgaatgt ccgtcatgcc gagcggcagg tccggatact tcag
//
LOCUS       DASRZF010000175         4848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4587334, whole genome shotgun sequence.
ACCESSION   DASRZF010000175 DASRZF010000000
VERSION     DASRZF010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 4848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4848
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4587334"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VFG22_07880"
     CDS             <1..201
                     /locus_tag="VFG22_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416202.1"
                     /translation="RYLEVFEVAKDHARSNPGGSQLVAYRSLARWGGMHSMVAGMGLM
                     RKPVQLNRNHAPTGTWTLSAAS"
     gene            complement(248..3523)
                     /locus_tag="VFG22_07885"
     CDS             complement(248..3523)
                     /locus_tag="VFG22_07885"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET6416203.1"
                     /translation="MKDLNELDGFDPDKERGILAWAAKNSVAANVLMLILIVGGVVTL
                     ASGIKQEVFPEVELDLVSISVIYPGASPAEVEQAVVLAVEEAVRGIDGVKKVTSSAFE
                     GQASTLVELLLNTDKDRALNDVKSAIDRITSFPEDVERPTVSLLSNRKQVISLVLYGD
                     VDEATLRAVAENSRRELLQDPRITYIELSGIRPLEISIEVPQAQLRKYGLTLDEIAAR
                     VRAASVELPGGGVKTQGGEVLLRTTERRDRGTEFGEIVLLSESDGSQVRVSDLATVRD
                     GFRETDKEATYNGKPAVMINVFRVGNETPLTVSAAAKEYVAKLQATLPEGLYAANWFD
                     TAEMYEGRLSLLLRNARIGLVLVLLTLALFLEARLAFWVTLGIPISFAGSLLFFPLAE
                     VSINMISLFAFIITLGMVVDDAIVVGEAIYKQRTDGKGRLQAAIDGAKEVAQPVIFAI
                     LTSCVAFAPMLMVPGPMGKFFRVVPIVVISVLLISLAESLLILPAHLAHPMPWWLRVV
                     LSPYLWVMRFIGKLDIPHRLQHHIQHTYLPVLEKALRWRYFTIAAGVALLIATMGYVA
                     GRIPFTFLPKVEGDLITAHLKMPVGTPVSETERIADGIAAAAQSIVDEEDATLESGTI
                     ARGLYEEIGAISSLEANPEGAIANEGSHMATVMMYLIDAGERELTTQQFVQRWRDEIG
                     EIPGADSLTFAFEVGVQPGKPIDIQLIHDDVPTLEAAAERLASEISSYSGLRDIDSGV
                     TKGKDQLDFRLTDAALSQGLTEIELARQVRGAFFGAEAVRQQRGRDELRVYVRLPLED
                     RRSLYNVEELIVRTPMGGEMPLAQAAIVERGQAYTVIKRTDGRRNISVTADVADEDAN
                     ANEINAQIIRRELAQLMADIPGLTYSLGGEQERQAETMGALGLGFALALIVMFSMLAV
                     VFRSYGQPILVMVAIPFGMVGAVWGHVVMGFSLSLMSMMGVVALSGVVVNDSLVLIDA
                     INRFREEGMGIWEAVVSGAARRFRPILLTSLTTFFGLAPMILEKSVQARFLVPMAVSL
                     GFGVLLATFITLLIVPCSYIILDDAQRSSGNLSARLRGRPTVPPPPPTIPTDAEVELL
                     TAE"
     gene            complement(3520..4734)
                     /locus_tag="VFG22_07890"
     CDS             complement(3520..4734)
                     /locus_tag="VFG22_07890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET6416204.1"
                     /translation="MSKRSERRLKWALSIGVVVAAVAAAVILVATKAEPPRAEKPLEG
                     TLVDVIQIGARRHDVDLHAKGTVVPAKEVVLQAELGGRVIWQSPDLVAGGRFKEGQPI
                     VRIDPRDYQLAVESARSQVNQAKLEMSLEKRRGEVAKREWNAFGEVDVSEEQRALAQR
                     EPQLESTRLALKAAQSALKKSQLDLTRTTLRAPFNAMVVSEGVDTGQLVSPQSSVARL
                     VGTDEYHVQVSVPVSALRTVAARTGDSPGSAAKIIQRAGAETIERPGEVIRQLPDLDP
                     GGAMARLLVRIENPLGKDGDLPLLLGSFVDVEIEAEPIENAIRLPRVALRNGRNVYVM
                     NDDDTLEIRDVEIAWTEPEAVLVTGGLEANERVVTSRIPTPVPNMLLRTLAPTPEPEP
                     ASPSAPAAQATP"
BASE COUNT          894 a         1548 c         1498 g          908 t
ORIGIN      
        1 cgctatctcg aggtgttcga agtcgccaaa gatcacgccc ggagcaatcc gggcggctca
       61 cagctcgtcg cctaccgcag ccttgcccga tgggggggga tgcattcgat ggtcgccggg
      121 atggggctca tgcgaaagcc ggtccagctg aaccgcaatc acgcccccac cggtacctgg
      181 accctctctg cggcttcctg acacgtgcga accactccga gtctccgagt cgccgccagc
      241 gtgctcgtca ctcggccgta aggagctcga cctcagcgtc cgtcggaatc gtaggcggtg
      301 gcggtggcac cgtcggccgg ccgcggagcc gcgcggacaa attgcccgag ctgcgctgcg
      361 catcatcgag gatgatgtag ctgcacggca cgatcagaag cgtgatgaat gtcgcgagga
      421 ggactccgaa ccccaaagat accgccatcg gcaccaggaa gcgtgcctgg actgattttt
      481 cgaggatcat cggagccagt ccgaagaagg ttgtcagtga cgtcaggaga atcggacgga
      541 agcggcgtgc agcacccgaa accacagcct cccagatacc cattccttcc tcgcgaaaac
      601 ggttgatcgc gtcgatgagg acgagcgaat cgttgacgac gaccccggac aacgcgacca
      661 cgcccatcat gctcatgagg ctcagcgaga agcccatgac cacgtgaccc cataccgctc
      721 cgaccatgcc gaatggaatc gccaccatca ccaagatcgg ctgcccgtag cttcgaaaca
      781 ccacggccag catcgagaac atcacgatca gggccagagc gaacccgagc ccgagcgcgc
      841 ccatcgtttc ggcctgtcgc tcctgctccc ctccgaggct gtaggtgagc ccagggatgt
      901 ccgccatgag ctgcgcgagc tcccgtcgga tgatctgcgc gttgatctcg ttggcattgg
      961 cgtcttcgtc tgcgacgtct gccgtgacgc tgatgttgcg gcggccatcg gttcgcttga
     1021 tcaccgtata ggcctgaccc ctctcgacga tggcagcctg cgcgagcggc atctctccgc
     1081 ccattggggt gcgcacgatc agctcttcga cgttgtagag cgagcgcctg tcttcgagcg
     1141 gaagacgtac gtacacgcga agctcgtccc ggcctcgttg ctgccgcacc gcttcggccc
     1201 cgaagaacgc gccgcgtacc tgtctggcga gctcgatctc ggtgaggccc tgggacaagg
     1261 ctgcgtcggt cagacgaaag tcgagctggt ccttgccctt ggtaacaccg ctgtcgatgt
     1321 cacgcagtcc gctgtacgag gagatttcgg aggcgagtcg ctccgcggcc gcttcgagcg
     1381 tgggcacgtc gtcgtgaatg agctgaatgt cgatcggctt tcctggctgg actccgacct
     1441 cgaacgcgaa cgtgagcgaa tccgcccccg gaatctcgcc gatctcgtcg cgccatcgtt
     1501 gcacgaactg ctgagtggtc agctctcgtt ctcccgcatc gatgagatac atcatcacgg
     1561 tcgccatgtg gctaccttca tttgcaatgg cgccttcggg gttggcctct aagctgctga
     1621 tcgcgccaat ctcctcgtac aagccacggg cgatggttcc gctttccaat gtcgcgtctt
     1681 cttcgtcgac gatcgactgc gcagccgccg caatcccgtc tgcgatacgt tcggtttccg
     1741 acaccggggt gccaaccggc atcttcaggt gtgccgtgat caaatcaccc tcgaccttcg
     1801 gaaggaatgt gaagggaatg cggcccgcca cataccccat cgtcgcgatc aagagcgcaa
     1861 cgcctgcggc gatggtgaag taccgccaac gcagggcctt ctccaagaca ggcaggtagg
     1921 tatgttggat gtgatgctgc aggcggtgcg gtatgtcgag cttgccgatg aagcgcatga
     1981 cccagagata gggcgagagc acgactcgga gccaccatgg catgggatgc gcgaggtgcg
     2041 ccggcaggat tagaagcgac tcggctagcg aaatcaaaag gacggagatg accacgatcg
     2101 ggacgacgcg gaaaaatttc cccatgggtc cggggaccat gagcatcggc gcaaatgcca
     2161 cgcagctcgt tagaatcgcg aagatgacgg gttgagcgac ctctttcgcg ccatcgatcg
     2221 ccgcctggag gcgtcccttt ccgtccgtgc gctgcttgta gatcgcctcg cccacgacga
     2281 tcgcatcatc gaccaccatg ccgagcgtga tgatgaacgc gaaaagcgag atcatgttga
     2341 tggacacttc cgcaagcggg aagaagagca gggagcccgc gaatgagatc ggaatgccca
     2401 gggtgaccca gaatgcgaga cgcgcctcga ggaacaaggc tagcgtgaga agcaccagca
     2461 cgaggccgat gcgcgcattc cgcaagagca acgagagtcg tccctcgtac atctcggccg
     2521 tatcgaacca gttcgccgcg tagaggcctt ctggcagagt cgcctggagc tttgccacgt
     2581 actccttcgc cgcggcggag acggtgagag gggtctcgtt gcccacccga aagacgttga
     2641 tcatcacggc gggtttgccg ttgtaggtgg cctctttgtc tgtctctctg aaaccatcgc
     2701 gcaccgtcgc caggtcgctc acgcgcacct gagaaccgtc cgattcgctc aagagcacaa
     2761 tctcgccaaa ctcggtgcca cggtctcgcc gctccgtcgt tcggaggagc acctcgccgc
     2821 cttgcgtctt cacgccgcca cccgggagct cgaccgatgc cgcgcgcacc cgggcggcga
     2881 tctcgtcgag cgtgagcccg tacttgcgga gctgggcctg cggcacctcg atgctgatct
     2941 cgagtggccg aattccggag agctcgatgt aagtgatgcg cgggtcctgc aggagctcgc
     3001 gacggctgtt ctcggccacc gcacgcaggg tggcttcatc gacgtcacca tagagcacca
     3061 acgagatgac ctgcttccgg ttcgaaagga ggctgaccgt cggacgctcc acgtcttcag
     3121 ggaacgaggt gattcgatcg atcgcgctct tcacgtcgtt gagggcgcgg tccttgtcgg
     3181 tgttcagcag gagctcgacc aaggtgctcg cctgcccttc gaaagccgac gaggtgactt
     3241 tcttcactcc gtcgatgcct cgaacggcct cttccactgc gagcacgact gcttgttcga
     3301 cttctgcggg gctcgcgcct ggatagatga cgctgatgga gaccaaatcg agctcgacct
     3361 ccggaaagac ctcctgcttg atgcccgagg ccagggtcac gacgcccccg acgatcagga
     3421 tcagcatcag cacgttcgcc gcgacggagt tcttggccgc ccaggcgaga attccgcgct
     3481 ctttgtccgg atcgaagccg tccaattcgt tcaaatcctt catggtgtgg cttgagccgc
     3541 aggcgcactc ggactcgcgg gctccggctc gggcgtgggg gctaaggtgc gaagcagcat
     3601 gttggggacg ggggtcggga ttcggctcgt cacgactcgc tcgttggctt cgagcccgcc
     3661 ggtgacgagc acagcctcag gttcggtcca ggcgatctcc acgtcccgaa tctcgagcgt
     3721 gtcgtcgtcg ttcatgacgt agacgttgcg accatttctg agagcgactc tagggagccg
     3781 gatcgcattc tcgatcggtt cggcctcgat ctcgacgtcc acgaaggatc cgaggagcaa
     3841 gggtaggtcg ccgtccttgc cgagtgggtt ctcgatccga accagaagtc gcgccatcgc
     3901 gccgccggga tccaggtcgg gaagctggcg aatgacctcg ccgggccgct cgattgtctc
     3961 agcgcctgcc cgctgaatga tcttggccgc ggaacctggg ctgtcgccgg ttcgtgcggc
     4021 gacggttcgc aacgcgctca ccggcacgga aacctgcacg tgatactcgt cggtgcccac
     4081 cagtcgtgcg acggagcttt gcgggctgac aagctgcccg gtgtccacgc cctcgctcac
     4141 gaccatcgcg ttgaagggcg cgcggagcgt cgttcgtgtc aagtcgagct gggacttctt
     4201 gagagcgctc tgcgcggcct tgagcgcgag ccgggtcgac tcgagctgtg gctcacgttg
     4261 cgccagcgcg cgttgttctt ccgaaacgtc cacttctccg aaggcgttcc actcccgctt
     4321 cgcgacttcg ccacggcgct tctccagcga catttcgagc ttggcttggt tgacctggga
     4381 gcgagcggac tcgacagcga gctggtagtc gcgtggatcg atgcgaacga tcggctgacc
     4441 ctccttaaag cgtccgccgg cgacgagatc cgggctctgc caaatcacac gccctccgag
     4501 ctcagcctgg agtacgactt ccttcgcagg taccacggta cccttcgcgt gcagatcgac
     4561 gtcgtggcgc ctcgcgccga tctgaatgac gtcgaccagt gtaccctcga ggggcttttc
     4621 ggcgcgcggc ggctcggctt tcgtcgccac caggatgacc gcggcggcga cagcagcgac
     4681 aacgacgccg atcgacagtg cccatttcaa tcgacgctca gaacgcttac tcattctctg
     4741 ggtctccaaa ttcttccggc catgcgccac cgagcgctcg atagagctgc acgcgataag
     4801 aaacgagtcg ccgacgcgag gccacgagtt gaagctccga gagctgac
//
LOCUS       DASRZF010000176        14516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2868980, whole genome shotgun sequence.
ACCESSION   DASRZF010000176 DASRZF010000000
VERSION     DASRZF010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 14516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14516
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2868980"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VFG22_07895"
     CDS             complement(<1..219)
                     /locus_tag="VFG22_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416205.1"
                     /translation="MDKKNNFWLMVVAAMMFGLLGCDSSSDGAAGMGGTGGTAGTGGT
                     AGDGGTGGRGGTDPGMAMVRVAHLAPEIP"
     gene            405..1292
                     /locus_tag="VFG22_07900"
     CDS             405..1292
                     /locus_tag="VFG22_07900"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET6416206.1"
                     /translation="MNQEAKYRVGMVSKMTGLSTHTLRIWEKRYAAVVPKRTEAGGRL
                     YTDADVERLRLLHELVQSGHSIGGIARLPDVDLRHMAAQFPQASTQPALQHLPEVRDR
                     VLAAIERLRVEEAEQVLSRAALSTEPREFLIGVVAPVMTEVGDRWERGELRIAHEHAC
                     STVMRGLLFSLMRLYPSSDARRRVVVATPASEDHELGALMVAMLAAMHGWGVLYLGTD
                     LPAEEIAYAVAKTGADLLMLSITNLDPEASERELAAIERAVPARVKILAGGQAAKALP
                     ESRIEIQQDLAIAERELSR"
     gene            complement(1282..1923)
                     /locus_tag="VFG22_07905"
     CDS             complement(1282..1923)
                     /locus_tag="VFG22_07905"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6416207.1"
                     /translation="MPGGFLIVEDEPTLARTLARTFGRHGPVEVVYSMAAAREQLQNH
                     DEWTGVVLDLILPDGYGLDLLAEIRQIHGALPVLVLTGELNRDVVNKVQTMRGEYVCK
                     PASVDNLNPFIQRALAQERVDDAAIGQRIEHLARQLKLTRRETELLTLSVDGLSRKEL
                     ADALGVAENTVKAQIRSLLKKSGARSLATLAQKVIKGATRDSVPPGEADEPSD"
     gene            2146..3795
                     /locus_tag="VFG22_07910"
     CDS             2146..3795
                     /locus_tag="VFG22_07910"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HET6416208.1"
                     /translation="MTAGDMYSVVITEEGGSRRKLDFSKPELTVGRVQGNDIVLSKRN
                     VSKQHARLLLKGEQAVVVDLNSTNGTWVNGRKITSAQPLKQGDKIYIADFIITLEPAN
                     DDPQHASSVPSVSEPPPLPSKRAAEPSAMPARPAREAQRPAPPVSRTPSPVDVRATSL
                     PPRIESRPTDAPRASARTETGFLAADPLHALLSQLAERIDIENVVPAAMKNQERWSAA
                     RAAIAETFLAMQTEGSVNADVDMRHIAHVALHEAVGLGPLDDLLSDESVRTIVVNGPD
                     HLFVDKGEGIEAVDREFSSEKALRRIARRLAAETGQALADQQVLHGRLSFGPRLTILQ
                     SPLVTKGPVIEIRIWSSKSLGELAEAGWMSSDAATYLSKAVAECRNIVVAGPRGSGVT
                     ELMSALARELSETENAVAIETAPDLDIDRNRVVSLTAADAGISLAEAIDHGTRLQSEH
                     LIINDVRGADTMGALSAVLGREPGHLIGVHCWSSKNAIEGLMLAAGCGGAERALIAQL
                     VGSAVDLVIGMRRGPDGQRVGGILEVQGHEAGDVSYQSVPF"
     gene            complement(3806..4534)
                     /locus_tag="VFG22_07915"
     CDS             complement(3806..4534)
                     /locus_tag="VFG22_07915"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET6416209.1"
                     /translation="MKSLPGVLARGDLSVVYQPIVSLSTMKPFAYEALVRSDSPHWTN
                     PPTLFDEAIKAGCVGALGRSIRELATTHCGGVPLFLNVHPNEFDESWLVQPDDPIFTH
                     DEPIYLEVTESVPLTHFAYCHSVLREIRGKGVSLAVDDLGAGYSNLKYIVDLKPEIVK
                     LDRTLIADLADDRRAQVLVRHLVKLCQELGAKVVAEGIEQETEMKVVIDCGAHYGQGY
                     YFARPAYPAPGVSATPRTVMRKGA"
     gene            4708..5739
                     /gene="fbp"
                     /locus_tag="VFG22_07920"
     CDS             4708..5739
                     /gene="fbp"
                     /locus_tag="VFG22_07920"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HET6416210.1"
                     /translation="MSSRVSIGPAEPSPIGTTLERHLLDRQRRMAEATGDFTGLFQQI
                     TLAAKIIGSRVSKAGLSGILGRTGTTNVQGEQVQKLDDFANTTIVSTVEAGGHVCVMG
                     SEEVEEPIAIPAQYPQGKYVLQFDPLDGSGNIDINASIGTIFSIHRRLSPEGGPGSLR
                     DLLQPGRDIVAAGYIVYGSSNMLVYSTGDQVDGFTYDPGVGEFFLSHPKIRIPTRGGI
                     YSINEGNYHSWDTGIRNWVDWIKTPSETKPAYRLRYIGAMVADLHRTLLRGGIFVYPS
                     DAKNRKGKLRLLYEASPMAFLVEAAGGTASDGKNRILDIAPTALHQRTPVFMGSPDDV
                     ADALRFAAS"
     gene            complement(5744..6334)
                     /locus_tag="VFG22_07925"
     CDS             complement(5744..6334)
                     /locus_tag="VFG22_07925"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HET6416211.1"
                     /translation="MTQGSSLPPLKKNDGKYGLVALLLLLSAGGLWLFLKPAGGDSEP
                     QAEVAAPAPEVAREQFAPEIEIPELDAAIDGVEEQEPSREAPPSPDDRPADWICDGTL
                     SASQLSEVIRGQPSKQVQTCYEQRLKENNLLQGSMTVLLTIGASGSVKAVSLSGSLGD
                     PQVYACVKRIAKTWKFPQPEGGCVRTAVPFQMTPKL"
     gene            6503..8110
                     /locus_tag="VFG22_07930"
     CDS             6503..8110
                     /locus_tag="VFG22_07930"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET6416212.1"
                     /translation="MIDKFKGFFLVLLVVLLSAVFALQFGGGQAEGCAAGGSTYLARI
                     YDQTLSRGDFEAAYAVANFGRLPEETQRSMRLPELVLDGLIDRTLLAREARKVGFDVD
                     QDEVMVRFVNEGIILLTLGVGAPPMLPQGEIPVSFTDKDGAFNKDLAERYIQNGLRRS
                     VGEFADAQVDEYLAAQMRELISNSVNVSEAEVWDDYVRENDNAKIKYVRFSPAYYAQE
                     KPPISESALDAWMTENDARLVAEYEANKHRYTNLEKQVRARHILVKAGSYASAEQKAE
                     AKKKAEALQMRAAKGEDFVALAKAQSDDPVTAKNGGDVGFLRKGAMPEAFDEALFAMK
                     VEEVSEVVETPYGFHVIKVVAIREGDVPVDEAKRELAEGLYRSDWLEARARDAAASTL
                     RSWKERQNDDEVAEKLTADGQKRGDSALTPTLEETTDFGRSDTPVPGLSTSALLDAVF
                     ALPEGEVFPDQPVKLGREWVIFRLIERQRPDEKAFTDAVRESTREVLLTLKRRETVDL
                     YIQQLRARATADKALRVNPLSTEDGRS"
     gene            8115..9401
                     /locus_tag="VFG22_07935"
     CDS             8115..9401
                     /locus_tag="VFG22_07935"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET6416213.1"
                     /translation="MSLPELPTESLTLGNGLRLRLIHLPHLQSATVSFFIRVGSRYES
                     QPTNGLSHFLEHMLYRGTKMHPAAHELSLAIERLGGTLDASTHVDFTSYDLTLPSETI
                     AEGVGLLAEVLQKPLLKELRTEKQIIREEILEELNEDGKQIDIDNVSRQLLYPDHALG
                     FTIAGPLENLARFDSEDLRRHHAAFYSARNSVLCVSGAFDPREIEDTVRSSFGAMPAG
                     EAIASGSKPVNAATERFDYVLEHGSQTDVRLSFHTPGAASPEAPTLTLLSRVLDDGLS
                     TRVHQIICEERGLAYEAFAGNDTYEDCGVFDFGASVEHKKAPLLVETVFELIDEIRRH
                     APSDEEIEKAKRRYLWDLRMVRDDPEGSAGFVGSSALFGLPERIEVIAEQVARVTADD
                     VQRAVQSYFDPQDAHVTCVGVLDEALRSDVRGLAGA"
     gene            complement(9398..10069)
                     /locus_tag="VFG22_07940"
     CDS             complement(9398..10069)
                     /locus_tag="VFG22_07940"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HET6416214.1"
                     /translation="MRVEVHYYAAARELAGCESSTFDFEPQSVPQSDLRAAVVDRFPS
                     LAAFLERMRLAINGDFVDASHAVQDGDRIDILPPVAGGSPIVLSEISAEKISLDEVRK
                     AVEHPAAGGVCVFHGVVRDHSDGQAVARLDYEAHESLAKKEMTRVLEGVAAEHPGVRI
                     AAVHRIGQLDIGDVAVCVAVSAAHRDDAFHACRKAIDRIKETVPVWKKEWGPDGRAHW
                     VNLDA"
     gene            10139..10969
                     /locus_tag="VFG22_07945"
     CDS             10139..10969
                     /locus_tag="VFG22_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416215.1"
                     /translation="MTASGLKLILWVVAASVAGTMLAIPQPVDPWEMPSLVLDRVAVS
                     DAIALDQTLAADAPESEEAEALRALFLDHGRSEANQPYERAEYEQRQIAIHHAMDAVT
                     KKHGAQALAALRAEAVETFMQQFGDGTRKPETDQGIAVLGGAPKILERYGAIYRGVLV
                     APPLTARVFYKARWNAVHRRPHVEDFSDIELQAYWGWFALHGWGKPLDQREEALLAFR
                     DAGGVGTEEAAALLDLLSGHPDRAARSLEKQYASSGELRLRNLALGILHAGLLPAGAP
                     "
     gene            11049..11828
                     /gene="pssA"
                     /locus_tag="VFG22_07950"
     CDS             11049..11828
                     /gene="pssA"
                     /locus_tag="VFG22_07950"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HET6416216.1"
                     /translation="MKVDLKKSLFILPNLFTLSSVLCGYYSILILSDERTNDDFYRAA
                     LLIVLALFFDGIDGRVARLTKTQTAIGVQLDSLADVISFGVAPAILVYHWSLAEFGTA
                     GMLIGFLFVAAGAIRLARFNVSSTGDDGEPHEPDKYTQGLPIPAAAVILISIVVANTL
                     TGKLPFSPFIISVLVITLSVFMVSAMPFRSFKDLRLNWRTVLFIGVTLSVTVWIALKI
                     HPSMALVWLLAAYVIIGIVESLIVLMNKARAGIEKASERSS"
     gene            complement(11825..13486)
                     /locus_tag="VFG22_07955"
     CDS             complement(11825..13486)
                     /locus_tag="VFG22_07955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET6416217.1"
                     /translation="MIDARALRGRDQLVGALLGIGYVLLLVKTAPDLAMSRDESFYVH
                     AAKDYGAWLSQLFSEPGLALARESIDRAWDYNWEHPAWMKLSFAVSWLVQKHWALFPS
                     ESLAFRFPAILTAGLLLWLIYSWGASVMRREGALFAALAFALMPRVFYHSHLACFDVP
                     IAFFVTLTTYAYWRALGDRRWVPIVGLAFGLALATKHNSWMLPAIFLIHFIWLRADEA
                     RSERSGRVSIAWLPSMLLVGPLVLIGTWPWLWNDTWARLLAYARFHLRHVHYTYEYFG
                     VSYFEPPLPVSVPFVMTLFTVSLTVLALAALGLYDRRSALRFSWRGESGGELGRRTEI
                     LWLGCMLAPLLAIALPSSPIFGGTKHWITAYPFLALFAGRGLLSVLERPELDVWTGRH
                     WPSWGLATLFLLPGAVETAHSHPFGLSHYMPLAGGVPGAADLGMNRQFWGFTTGSVAG
                     WIARRLPEGGSVWICDTTHEAWLMMQRDGLIPGNIRAVSSMRSADLVLVHHEAHFSEV
                     DHQAWVAFGNVRPVHVLLYDGVPIISIYEKPTGRGVDRAERPIDD"
     gene            complement(13479..14417)
                     /locus_tag="VFG22_07960"
     CDS             complement(13479..14417)
                     /locus_tag="VFG22_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416218.1"
                     /translation="MAVAGRKRGLAPWTWVLVAAIAVLELAAHAWIVAAIPREQSWEA
                     ASAFVRARFRAGDRIVAAPAWVDPIVRNQLGDLLSLRSAAAVDLGGVDRIWELSIRGA
                     EERRESVELDQRFGAVRVRMWPLLSEPLIYDFVEEVARAKVELLGAEGSKPCAWMRAR
                     SSSGGLGQGPTTPEERFVCDPRRPWLWVGATVLTDLDFHPRRCVFQHPTGVDPVRVTF
                     QDVPLGERLVVHGGIQYESERSRQHDPVTLRVWIDGRLAGELVHQDGDGWTGMEIDTS
                     ALARERAEVRFETTTSNPKARLFCWSASSRSAARHD"
BASE COUNT         2821 a         4542 c         4514 g         2639 t
ORIGIN      
        1 gggtatctcg ggggccagat gcgcgactcg caccatcgcc atccccgggt ctgttccccc
       61 tctgccgccg gtgccgccat cgccagcagt gccaccggtt ccagcagtgc caccggttcc
      121 gcccatgccg gcggcgccat cactgctgct gtcgcatcca aggaggccga acatcattgc
      181 cgccaccacc atcaaccaaa aattgttctt tttatccatt agactcctcc gagttgtcta
      241 agttacgtcc aggttctgtc caggtctata acgatgtgga gcgctgcgtc aagtcgcctg
      301 cgtgaatttt tgtcctgaac gggcttgaac gttggaatca caagcaatag acaagtctta
      361 gacagtggct acccaccgtc ccaggggctt ccttagaaat cgagatgaat caagaggcga
      421 aataccgggt cggcatggtc tcgaagatga cggggctctc gactcacacg ctccggatct
      481 gggagaagcg ctacgccgcg gtcgtcccga agcgcacgga agcaggaggt cggctctaca
      541 cagacgctga cgtcgagcgg ctgcgcctac tgcacgagct tgtccagagt ggccattcga
      601 tcggcggcat cgcgcgattg ccggacgtcg acctccgaca catggccgct cagttcccgc
      661 aggcctccac tcaacccgcg ctgcagcatc ttcccgaggt gcgtgatcgt gtgctggccg
      721 cgatcgagcg actccgggtc gaagaagcgg agcaagtgct ctcgcgcgca gcgttgagca
      781 cggagccgcg cgagttcctg atcggcgtcg tggccccggt gatgaccgag gtcggcgatc
      841 gctgggagcg aggggagctt cgcatcgcac acgagcacgc ttgctcaacg gtcatgcgag
      901 ggcttctctt ctccttgatg cgtctgtacc cctctagcga cgcgaggcgc cgggtcgtgg
      961 tagcgactcc cgcatcggag gatcacgagc tcggcgcgct catggtcgcg atgctcgctg
     1021 cgatgcacgg atggggcgtg ctctatctcg gcacggatct accggctgaa gagatcgcgt
     1081 acgccgtggc caagaccgga gcggaccttc tcatgctctc catcaccaat ctggatcccg
     1141 aagcctcgga gcgggagctc gccgcgatcg aacgggcagt gcctgcgcgt gtgaagattc
     1201 tcgcgggtgg gcaggcggca aaggcgctgc ccgaaagccg catcgagatc cagcaagacc
     1261 tcgcgatcgc cgagcgcgag ctcagtcgct aggttcgtcg gcttcgccag gcggaaccga
     1321 gtcgcgcgtg gcccctttga tcaccttctg cgcgagcgtc gcgaggctcc ttgccccact
     1381 cttcttcagg agggatcgaa tctgtgcttt gaccgtgttc tcggcgacac caagggcatc
     1441 ggcgagctcc ttccgagaga gcccgtcgac cgagagcgtg agaagctcgg tttcgcggcg
     1501 tgtgagcttg agctgacggg ccagatgctc gatgcgttga ccgatcgccg cgtcgtccac
     1561 gcgctcttgc gcgagcgcgc gctggatgaa tgggttcaga ttgtcgacgg aagcgggctt
     1621 gcagacgtac tcgccgcgca tcgtctgaac cttgttgacc acatcacgat tgagctcgcc
     1681 cgtcaggacc agcaccggca atgcgccatg gatctgccga atctcggcca gcaaatcgag
     1741 tccgtacccg tcgggcagaa tgaggtccaa gacgacaccc gtccattcgt cgtggttctg
     1801 caactgctct cgggccgccg ccatgctgta cacgacctcg accggtccgt gccgcccaaa
     1861 cgtccgcgcg agggtccgag ccaacgtcgg ctcatcctcg acaatgagaa accctcccgg
     1921 catgcggcgt aagctaaccc agttgacccc ttcggtggta ccgattccct cctcttggcg
     1981 cacattttta acccttatct gctcggaatc gtggaagcag agcagaaatg cttcatattt
     2041 tcatatattt atagaaatag gccgggaggc ttagctcacg cgcaggcctc agccgacggg
     2101 gcgagaatcg gcagtctctt gacgccgggg ttcggcgcgc tttgaatgac cgcgggggat
     2161 atgtactcgg tcgtaatcac agaagaaggt ggctctcggc ggaagctcga tttctccaag
     2221 ccggagctga ccgtgggtcg cgttcagggg aacgatatcg ttctgtcgaa gcgcaacgtg
     2281 tcgaagcagc acgcgcgctt gctgctgaag ggcgaacagg ccgtggtcgt cgacctcaac
     2341 agcacgaacg gcacctgggt caatggccgc aagatcactt ccgcccagcc cttgaagcag
     2401 ggcgacaaga tctacatcgc cgacttcatc atcacactcg agccggcgaa cgacgatcca
     2461 cagcacgcga gctcggtgcc gagcgtctcc gagccgcctc cgcttccgtc caagcgagca
     2521 gccgaaccga gtgccatgcc tgcgagaccg gcacgcgaag cacagcgccc ggcgccgccg
     2581 gtctcgagaa cccccagccc cgtggacgtt cgcgcgacga gccttccacc tcgcatcgaa
     2641 tcgcggccca ccgatgcgcc tcgagcgtcc gcacgaaccg agacggggtt tctggccgca
     2701 gaccctttgc acgcgctgct gagccagttg gccgaacgca tcgacatcga gaacgtcgtg
     2761 ccggcggcga tgaagaatca agaacgctgg tcggccgctc gcgcagcgat cgcggaaacc
     2821 ttcctcgcga tgcaaaccga gggctcggtg aatgccgacg tcgacatgcg tcacatcgct
     2881 cacgtggcct tgcacgaggc cgttggcttg ggtccgctcg acgatctgct ctcggacgag
     2941 tcggttcgca cgatcgtcgt caacggaccc gatcacctgt tcgtcgacaa gggcgaaggg
     3001 atcgaagcag tcgaccggga gttctcttct gaaaaggctc tcagaagaat cgcgcgacgg
     3061 cttgcggccg agaccggaca agctctcgcg gatcagcaag tgctgcacgg tcggctttcg
     3121 tttggcccac ggctcacgat cttgcagtct cctctggtca cgaagggccc ggtgatcgag
     3181 attcgcatct ggagctcgaa atccctcggt gagctggccg aagcagggtg gatgagcagc
     3241 gacgctgcga cctacctgag caaggccgtc gcggaatgcc gcaacatcgt ggtagccggg
     3301 ccgcgtggct ccggcgtcac cgagttgatg tccgcgcttg cccgggagct ctcggaaacg
     3361 gagaacgcgg tggcgatcga gactgctccc gacctcgaca tcgatcgaaa ccgcgtcgtt
     3421 tcattgacag cggccgacgc ggggatctcg ctcgcggaag cgatcgacca cggaacgcgg
     3481 cttcaatccg aacatctgat catcaacgat gtcaggggag cggacacgat gggtgcgctt
     3541 tcagcggtgc tcggtcgcga acccggccat ctgatcggtg tgcactgctg gagcagcaag
     3601 aatgcgatcg aaggcctcat gcttgctgcg ggctgcggcg gcgctgaacg cgccttgatc
     3661 gcgcagctcg tgggaagcgc agtagatctc gtgattggga tgcggcgtgg acccgatggt
     3721 cagcgcgtag gcggaattct agaggttcaa ggtcacgagg cgggcgacgt cagctaccaa
     3781 tccgtgccgt tctgagctga gcggctcaag cgcctttgcg catcacggtg cggggagtcg
     3841 cgctgacgcc aggggctgga tacgcgggac gcgcgaagta gtagccctga ccatagtgcg
     3901 ccccacagtc gatgacgacc ttcatctcgg tttcttgctc gatgccctcg gcgacgacct
     3961 ttgctccgag ctcctggcat agcttcacga ggtgacgcac caggacctgc gcgcgtcgat
     4021 catcggcaag atctgcgatc aaagtacgat cgagcttcac gatctccggc ttgaggtcga
     4081 cgatgtactt caagttcgag taccccgcac cgaggtcatc gacggcgagc gagacgccct
     4141 tgcctcggat ctcgcgaagc acgctgtggc agtatgcaaa gtgagtgaga ggaaccgact
     4201 cggtcacttc gaggtagatc ggctcgtcgt gtgtgaagat cggatcatct ggctgcacga
     4261 gccaggactc gtcgaactca ttggggtgca cgttgagaaa aagtggcacc ccgccgcagt
     4321 gcgtcgtggc aagctcgcga atcgagcgtc cgagcgcgcc gacgcatcca gctttgatcg
     4381 cctcgtcgaa gagcgtcggc gggttggtcc agtgtggcga gtcactccgg acgagggcct
     4441 cgtaggcaaa aggcttcatg gtcgagaggg agacgatcgg ctggtaaacg acgctgaggt
     4501 ccccccgggc gagcacgcct ggcagcgact tcatgctgcg gaccttcggg agccccgatc
     4561 gccgttgctg cgtcgcctca agccagttgc ggaagttcgt cacagatgcc ctaacacggt
     4621 aagtttacaa tatcacagac ccgaattgat atcctaattg tgggatgcct agcgcttcta
     4681 cgggggctct ggtatgcccc cgcagctatg agcagccgag tatcgattgg ccccgctgag
     4741 ccgagtccca taggcacgac ccttgaacga cacctgctcg accgccagag gcggatggcg
     4801 gaggcgacgg gcgatttcac tgggcttttc cagcagatca ccctggcggc gaaaatcata
     4861 ggcagccgcg tcagcaaagc agggctctcg ggcatcctcg gccgaaccgg cacgacgaac
     4921 gtccagggcg agcaggttca gaagctcgat gacttcgcca acaccacgat cgtgagcacg
     4981 gtcgaggcag gcgggcacgt ctgcgtgatg ggaagcgaag aggtcgagga gccgatcgcg
     5041 attcccgcgc agtatccgca gggcaagtac gttctgcagt tcgatccgct cgacggctcg
     5101 ggcaacatcg acatcaacgc atcgatcgga acgatctttt caattcaccg tcgcctctcg
     5161 cctgaaggcg gtccggggtc acttcgggat ttgttgcagc ctgggcgcga catcgtcgct
     5221 gcgggttaca tcgtttacgg ctcgtccaac atgctcgtct actccactgg cgaccaagtc
     5281 gacggtttca cttacgaccc gggcgtcggt gagttcttcc tctcgcaccc gaagatccgg
     5341 atccccacgc gcggcggcat ctattcgatc aacgaaggca actaccacag ctgggatacg
     5401 ggcattcgaa actgggtcga ctggatcaag acgccttcgg agaccaagcc ggcctaccgt
     5461 ttgcgctaca tcggcgcgat ggtcgcagac ctgcatcgaa cgctgcttcg gggcggcatt
     5521 ttcgtctatc cgagcgacgc caagaaccgt aagggcaagc tacgtctgct ctacgaagcc
     5581 tcgccgatgg cattcctggt cgaagccgca ggaggcaccg catcggatgg caagaatcga
     5641 atcctcgata tcgcgccgac cgcgcttcac cagcgcacgc cggtcttcat gggaagtccg
     5701 gacgacgttg cggacgcgct gcgcttcgcg gcctcatgac cgatcaaagc ttcggggtca
     5761 tctgaaacgg aactgcggtt cgcacacagc cgccttccgg ctgcgggaac ttccaggtct
     5821 tggcgatgcg cttcacgcag gcgtagactt gcgggtcgcc gagtgagccg ctcaaggaca
     5881 cggctttgac gctgccgctc gcgccgatgg tgaggagcac ggtcatcgat ccctgcagca
     5941 ggttgttctc cttgaggcgc tgctcgtaac aggtctggac ctgcttgctc ggttggcccc
     6001 ggatcacctc gctgagctgc gatgccgaaa gcgtcccatc gcatatccag tcggctggac
     6061 ggtcgtcagg gcttggcgga gcctcccggc tgggctcttg ctcttcgact ccgtcgatcg
     6121 ccgcatcgag ctcgggaatc tcgatttcag gggcgaactg ctccctggca acctcgggtg
     6181 cgggagccgc aacctcggcc tgaggttcgg aatcgccccc agctggtttt aggaacagcc
     6241 agaggcctcc ggccgagaga agaaggagta aggcgaccag cccgtacttc ccatcgttct
     6301 tcttgagtgg tggtaggctc gatccctggg tcacttcccc ctcactttcc ccggttcgag
     6361 gcgcgtagcg cgactgaagc ccgagtgtag attccctggc cgatcaagcc aactctcgag
     6421 acaccttggg gcatcgtaaa ccagagtttg ccgcaagccg acgtctcctc tatggttcgg
     6481 cctctttaaa tggcaattgg cgatgattga taagttcaaa ggcttttttc tggttcttct
     6541 cgtggtgctg ctttcagcgg tgttcgccct tcaattcggg ggtggtcagg ccgaagggtg
     6601 cgcagcgggt ggcagcacat atctggctcg catctatgac cagacgctga gtcgaggcga
     6661 tttcgaggcc gcgtatgccg tcgccaactt cgggcggctt ccggaggaga cgcaacgctc
     6721 gatgcggttg ccggagctcg tgctcgacgg cctgatcgat cgcacgctgc tcgcgcgaga
     6781 agcgcgcaag gtcggcttcg acgtcgatca agacgaggtc atggtgcgct tcgtgaacga
     6841 aggcatcatt ctgctgacgc tgggcgtcgg cgcgcctccc atgctcccgc agggcgagat
     6901 tccggtttcg ttcaccgaca aagacggagc cttcaacaag gatctcgctg agcgttacat
     6961 ccagaacggg ctccggcgaa gcgtgggcga gttcgccgat gcgcaggtcg acgagtactt
     7021 ggccgcgcag atgcgtgagc tcatcagcaa ttcagtgaac gttagcgaag cggaagtctg
     7081 ggacgactac gttcgtgaga acgacaacgc caagatcaag tacgttcgct tctcgcctgc
     7141 ttactacgct caagaaaagc ctccgatctc cgaatccgct ctcgacgcct ggatgaccga
     7201 gaacgacgcg cgtctcgttg cagagtacga agccaacaag caccgttaca ccaacctcga
     7261 gaaacaggtc cgagcgcggc acattctcgt gaaggccgga agctatgcct ccgcggagca
     7321 gaaagccgaa gccaagaaga aggccgaggc gcttcagatg cgcgccgcca agggcgagga
     7381 tttcgttgcc cttgccaagg cgcagagcga tgacccagtc acggccaaga acggcgggga
     7441 cgtgggtttc ctgcggaagg gcgcgatgcc cgaagcgttc gacgaggctc tgttcgcgat
     7501 gaaagtcgag gaagtctccg aagtggtcga gacaccctac gggttccacg tcatcaaggt
     7561 cgtggcgatc cgcgaaggag acgtcccagt cgacgaggcg aagcgcgagc ttgcggaagg
     7621 gctctatcgg tcggactggc tcgaagcgcg cgcgagagac gccgctgcga gcacgctgcg
     7681 ttcctggaag gagcgccaga acgacgacga agtcgcggag aagctgaccg cggatggtca
     7741 gaagcgcggc gattcagcgc tgaccccgac cttggaggaa accaccgatt tcggtcgctc
     7801 agacaccccc gttccaggcc tctcgacctc tgcattgcta gacgcagtgt tcgcgctgcc
     7861 cgaaggcgaa gtattccccg accagccagt gaagctcggt cgcgagtggg tgatttttcg
     7921 tctcatcgag cggcagcgtc ccgatgaaaa ggcattcacc gatgccgttc gcgagtccac
     7981 gcgcgaggtt ctgctgaccc tcaaacgcag agagaccgtc gacctctaca ttcaacagct
     8041 tcgagcgaga gccactgccg acaaggcgct tcgggtcaac ccgctctcga ctgaggatgg
     8101 tcgtagctaa cggtgtgagt ctccccgagc ttccgaccga gtcgctcacg ctcggtaacg
     8161 gccttcgctt gcgtctcatt cacttgcctc acctgcaatc tgccacggtc tcgttcttca
     8221 ttcgcgtcgg gtcgcgctac gagtctcagc caaccaacgg cctgagccac ttcctcgagc
     8281 acatgctcta tcgcggcacc aagatgcacc ctgcggcgca cgagctgagt ctcgcgatcg
     8341 aaaggcttgg aggcaccctg gacgcctcca cccacgtcga cttcaccagc tacgacctga
     8401 ccttaccctc cgagacgatc gccgaaggcg tcgggctgct cgcagaggtt cttcagaagc
     8461 ctttgttgaa ggagctgagg acggagaagc agatcatccg cgaagagata ttggaagaac
     8521 tgaacgagga cggtaagcag atcgacatcg acaacgtgtc acgccaactg ctctatccgg
     8581 accatgcgct cggattcacc attgcaggtc cgctcgagaa cctggcacgc ttcgatagcg
     8641 aggacctgcg ccgtcatcac gctgctttct actcggcgcg gaattcagtg ctctgcgtct
     8701 cgggagcctt cgatccacga gagatcgaag acaccgttcg ctccagcttc ggggcaatgc
     8761 ccgccggcga ggcgatcgcg tcgggcagca agcccgtgaa cgcagcaacg gaacgcttcg
     8821 actacgtgct ggagcacggg agccagacgg acgttcgcct ctcctttcac acgcctggcg
     8881 ccgcgagccc tgaggcccct actctcaccc tgttgagccg ggtgctcgac gacggcctca
     8941 gcacgcgcgt ccatcagatc atctgcgaag agcgggggct ggcctacgaa gccttcgcgg
     9001 gaaacgacac gtacgaagat tgcggcgtgt tcgatttcgg ggcttccgtg gagcacaaga
     9061 aggctcccct tctggtggaa accgtattcg agctgatcga cgagatccga aggcacgctc
     9121 cgagtgacga ggagatcgag aaagcaaagc ggcgctacct ttgggatctc cgtatggtcc
     9181 gtgacgaccc cgagggctcc gctggattcg tcgggagcag cgcgctcttt gggctacccg
     9241 aacgaattga ggtcatcgcc gagcaggtcg cccgagtcac ggcagacgac gtccagcgcg
     9301 cagtgcagtc ctacttcgat cctcaagacg cacacgtcac ctgcgtcgga gtgctcgacg
     9361 aagcgctgcg ttcggacgtt cgcggccttg ctggcgccta agcgtccaga ttgacccagt
     9421 gtgcgcggcc gtcgggaccc cactccttct tccagaccgg tacggtttct ttgattcgat
     9481 cgatggcttt tcggcaagcg tggaatgcat cgtcgcgatg cgcggcgctg accgctacgc
     9541 aaaccgcgac gtcaccgatg tcgagctgcc cgatgcggtg aacggccgcg atccggaccc
     9601 ctgggtgctc agcagcgacg ccttcgagca cacgcgtcat ctcttttttc gcgagactct
     9661 cgtgcgcctc gtagtctagg cgggccacgg cttgaccatc cgagtggtcg cgaactacgc
     9721 cgtggaaaac gcaaacacca ccggccgcag ggtgctcgac agcctttcgt acctcatcga
     9781 gggagatctt ctcggcgctt atctcgctca ggacaatcgg agagccgccg gcgaccggag
     9841 gcaagatgtc gatgcggtcg ccatcttgaa ctgcgtgcga agcgtcgacg aaatcgccgt
     9901 tgatcgccag gcgcatgcgc tcgaggaagg ccgccaagga ggggaaccga tcgacgaccg
     9961 cagcgcggag atcgctttgg ggtacgcttt ggggctcgaa atcgaacgtc gagctttcgc
    10021 agccggcaag ctcccgggcg gcagcatagt aatggacctc gaccctcatc gtgctggcga
    10081 agcctagcag gcatcgaact aggggcagcc gtgaggcgca tgttaggatt cgccccaggt
    10141 gaccgcctcc ggactcaagc tgatcctctg ggtcgtcgca gcaagcgtcg caggcacgat
    10201 gctggcgatt ccgcagcctg tagacccctg ggagatgccg agcctcgtgc tcgaccgggt
    10261 cgcggtgtcg gacgcaattg cgctcgacca gacccttgcc gcagacgcac ccgagagcga
    10321 ggaggccgag gccctgcgcg cactcttcct ggaccacggc cgctccgaag cgaatcagcc
    10381 gtacgaacgg gcggagtacg agcagcgcca gattgcgatt catcacgcga tggacgcagt
    10441 gaccaagaaa cacggtgctc aggcgctcgc ggctctgaga gccgaggcgg tcgaaacctt
    10501 catgcagcag ttcggcgatg gcacgcggaa accggagacc gatcagggga tcgcagtgct
    10561 cgggggagcg ccaaagatcc tcgagcggta cggcgccatc tatcgtggcg tgctggtcgc
    10621 gccgccgctc actgcgcgag tgttctacaa ggcccgctgg aacgcggttc accggcggcc
    10681 ccatgtcgag gacttctccg acatcgagct ccaggcctat tggggctggt ttgccctgca
    10741 tggatgggga aagcctctgg atcagcgaga agaagctctg ctggcgtttc gtgacgccgg
    10801 tggagtgggt accgaggagg cggctgcgct tttggatctg ctctccggac atccagatcg
    10861 ggccgccagg tccctagaga agcagtacgc atcgagtgga gagcttcgct tgcgaaacct
    10921 tgcgcttggc attctacacg caggcctttt gcccgcaggc gcgccgtgat acgagactcg
    10981 gagtggacag cgaattccgc ctcccgtagc ctacgggttc ggatcgagcg gaaagagtcg
    11041 agcctggcgt gaaagtcgac ctcaaaaaga gcctcttcat actgcccaat ctcttcacgc
    11101 tctcgagcgt gttgtgcggg tactactcga tcttgatctt gtccgatgag cggacgaacg
    11161 acgatttcta ccgtgctgcg ctgctgatcg tgctcgccct ctttttcgat ggcatcgacg
    11221 gtcgagtcgc ccggctcacc aagacccaga cggcaatcgg cgtacaactc gactcacttg
    11281 ccgacgtgat ttcgtttgga gtggcgcccg ccatcctggt gtaccactgg tcgctcgccg
    11341 agttcgggac tgccggcatg ctcattggtt ttctcttcgt cgcggcgggc gcgattcggc
    11401 tcgcgcgctt caatgtgagc tccaccgggg acgacggcga acctcacgaa cccgacaaat
    11461 acacgcaggg ccttccgatt cctgcggctg ccgtcatttt gatttcaatc gtggtcgcca
    11521 acacgctgac cgggaagctt ccgttttcgc cgttcatcat ttccgtcctg gtcatcacgc
    11581 tttcggtctt catggtgagc gcgatgccct tccggtcctt caaggatctg cgcctcaatt
    11641 ggcgaaccgt gctcttcatc ggcgtgacgc tgagcgtaac cgtgtggatc gcattgaaga
    11701 tccacccttc gatggcgctt gtctggctgc tggcggccta cgtgatcatc gggatcgtcg
    11761 agagcctgat cgtgctcatg aacaaggcgc gtgccggcat cgaaaaagcc agcgagcgct
    11821 cttcttagtc gtcgatcggc cgttcggccc gatccactcc gcgacccgtt ggcttctcgt
    11881 agatcgaaat gatcggcacg ccgtcataga ggagcacatg gaccggtcgg acgttgccaa
    11941 aggcgaccca ggcctgatga tcgacctcac tgaaatgggc ttcatggtgc accagcacga
    12001 ggtccgccga gcgcatcgac gatacggcgc ggatgttccc tggtatgaga ccgtctctct
    12061 gcatcatgag ccaggcctca tgcgtggtgt cgcagatcca aacagacccg ccctccggaa
    12121 gtctacgggc aatccatcct gcaacgctgc cagtcgtgaa cccccagaac tgccgattca
    12181 tcccgagatc cgctgcgccg ggaacacctc ctgcaagagg catgtagtgc gagaggccga
    12241 acgggtggct gtgcgccgtt tcgaccgctc cgggaagtag aaacaaagtc gcgagccccc
    12301 aggacggcca atggcggcct gtccaaacgt cgagctcggg cctttcgagc acgctcagaa
    12361 gcccacggcc tgcaaagaga gccaggaatg gataggcagt gatccaatgc ttggttcctc
    12421 caaatatcgg tgaggaagga agcgctatgg cgagcagcgg cgcaagcatg catccaagcc
    12481 aaaggatctc ggtcctgcga ccgagctcgc cccccgactc gccgcgccaa gaaaatcgaa
    12541 gcgcgctgcg gcggtcgtag aggccgagcg cggcgagcgc gagcaccgta agtgacaccg
    12601 tgaacagcgt catgacgaag ggcaccgaaa ccgggagcgg gggctcgaag tagctgacgc
    12661 cgaaatactc gtaggtgtag tgcacgtggc gaagatggaa tcgcgcgtat gcgaggagac
    12721 gcgcccaggt gtcattccaa agccagggcc aggttccgat caagaccaac gggccgacta
    12781 ggagcatcga aggcagccaa gcgatcgaga cccgtccgct ccgctcggag cgcgcttcat
    12841 cggcgcgaag ccagatgaaa tgaatgagga aaatcgctgg aagcatccaa ctgttgtgct
    12901 tcgtcgcaag cgcaagccca aacgcgagac ccacgatggg cacccatcgg cgatctccga
    12961 gcgcgcgcca gtacgcgtac gtggtcagcg ttacaaagaa cgctatggga acatcgaagc
    13021 aggcgagatg actatggtaa aagacgcgcg gcatgagggc gaacgcgagg gccgcgaaga
    13081 gcgctccttc tctgcgcatc acggaggcac cccaggaata gatcagccaa agcagcaggc
    13141 cggcggtgag tatcgccgga aatcggaagg cgagcgattc actcggaaac agagcccagt
    13201 gcttctggac gagccagctc acggcgaagc tcagtttcat ccacgccggg tgctcccagt
    13261 tgtagtccca ggcgcgatcg atgctctccc gggcgagtgc cagtcctggc tcgctgaaga
    13321 gctggctcag ccaagccccg taatccttgg ctgcgtgcac gtagaagctc tcgtcgcgcg
    13381 acatggccag gtccggtgcg gtcttcacga ggagcaacac gtatccgatg ccgaggaggg
    13441 cacctacgag ctggtcccgg cctctgagcg cgcgcgcgtc aatcatggcg ggccgccgat
    13501 cggctcgatg cggaccagca gaacagacgc gccttcgggt tcgaggtcgt agtctcgaac
    13561 cgcacctctg cgcgctcgcg tgcgagcgcg gaggtgtcga tctccatgcc cgtccagcca
    13621 tccccatcct gatgaacgag ctcgccggcg agccgcccgt cgatccagac ccggagcgtg
    13681 acggggtcat gctggcgact tcgctcactc tcgtattgaa ttccaccgtg cacgacgagc
    13741 cgctcaccga gtggcacgtc ctggaaggtg actctgaccg gatctacacc ggtcggatgc
    13801 tggaagacgc agcgcctcgg gtgaaaatcg aggtccgtca gcaccgtcgc acccacccag
    13861 agccagggtc gccgcgggtc gcagacgaag cgttcctccg gcgtcgtggg gccctggcca
    13921 agacctccac tagagctccg agcccgcatc caggcacagg gcttcgaacc ttcggcgccg
    13981 agcaattcga cctttgcacg cgccacctcc tcgacgaagt cgtagatcag cggctcactc
    14041 aagagaggcc acattcgaac acgaaccgcg ccgaatctct ggtccagctc gacggactcg
    14101 cgtcgctctt cggccccgcg gatgctgagc tcccagatgc ggtcgacacc gccgaggtcg
    14161 accgcggcag cgctccggag actgagcagg tcgccgagtt ggtttcgaac gatggggtcg
    14221 acccaagcgg gcgccgccac gatgcgatcc ccggcccgga agcgggcgcg gacgaaggcg
    14281 gacgctgctt cccaggattg ctcgcgagga atcgcggcga cgatccacgc gtgcgccgcc
    14341 agctcgagca ccgcaattgc ggccaccagc acccaagtcc acggagcgag gccccgcttt
    14401 cgccctgcta ccgccacgcc agcgcgatgc tagcagggct cagatcattc cgcagcttcg
    14461 cagaagaact cgaagctcca gccctcgagt gtcggagtga tcggcgggct gttcga
//
LOCUS       DASRZF010000177         2841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_117109, whole genome shotgun sequence.
ACCESSION   DASRZF010000177 DASRZF010000000
VERSION     DASRZF010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117109"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1403
                     /locus_tag="VFG22_07965"
     CDS             <1..1403
                     /locus_tag="VFG22_07965"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6416219.1"
                     /translation="RLGVGGMAETFEATRAGAGGFEQRVCLKRVLPAFCGDEEFVARF
                     RSEAKLAAQLRHTNIVGVIDFGEIDSVQYMSLELVDGLDLRSLLKSSPRERLPPDWVA
                     LIGTDLAYALEHAHESLVHRDISPSNILLSRSGEAKLADFGIAKALEDTAATASRSAK
                     GKVPYMSPEHMRGEAVDGRSDLFSLGVALFEAVAGCRPFDGAHDVETMQKILSGERLS
                     LEHVAPGAPGPLREVIERLIEVDRDARTPSASRLIEELAVLSPTPAVRRELAATVEEH
                     RGGPSARLHVRAKEEERDTELGRAPRPHADSAATESAATQKTKPRSKRRALILSAAML
                     TSLAAMIAGTQWLGSVPVSVPAPAPAPVSVPASVPAPVPVPVPAPVPASVPAPVSAPV
                     PEPAPRPSKGWLNVVVQPWGNVWIDGNWMGRAPVKARLPKGRHLVEAGRKRPIQKRVI
                     RVLAGSRKEVEFSLPD"
     gene            complement(1407..2690)
                     /locus_tag="VFG22_07970"
     CDS             complement(1407..2690)
                     /locus_tag="VFG22_07970"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HET6416220.1"
                     /translation="MRNWLSIVFVGLGGLGMLAACGGDGTGGPPKGGSGGSSGSVLLD
                     CSTLSNPPADCDTSCVSDSECAASYCENSTKCVANCTANEGCGAGSTCNTTRGRCVPD
                     VGTGGTGGTGNSIGCQSVEVTPSRSIPNVMFLVDQSGSMTSNFAGQERWTAAHGAITE
                     IVADLDSIVRFGLSTYTSDNGNSNLPCPRLPVAGDPFAVSPRVDFDLNNSSAISNNNI
                     YPSNYPSNAGGDTPTGDSIDALVSIIQSDPPPAEGPTIIVLATDGEPDTCEQPNPQNG
                     QAEAVAAATASHAAGLDVFILSVGNEVSDAHLQEMANVGVGKPANESGNPAPFWKATT
                     AELLAEAFQQIISDSISCEIEIDKPFNTDTLAQACDEGEVRLNGVLLDCPTDWQVKNE
                     PPYQTIELLGNACDTLKGGEVTFTAEFPCGTIVVE"
BASE COUNT          546 a          885 c          899 g          511 t
ORIGIN      
        1 gccggctcgg cgtgggcggc atggcggaaa cctttgaagc gacgcgcgca ggcgcaggtg
       61 gcttcgagca gcgtgtctgc ctcaagagag tcctgcccgc gttctgcgga gacgaagagt
      121 tcgtcgctcg atttcgcagt gaggccaaat tggccgcgca gcttcgacac accaacatcg
      181 tcggcgtcat cgactttggg gagatcgaca gcgtccagta catgagcctc gagctggtgg
      241 acggtctcga cctgcgatcg ctgctcaagt catcgccaag ggaaagactc cctcccgatt
      301 gggtggcact gatcgggacc gatttggcgt acgcgctcga acacgcacac gagagcctgg
      361 tgcaccggga catctcgccg tcgaacatct tgctcagtcg ctccggtgaa gcgaagctcg
      421 cggacttcgg catcgcgaag gctctcgagg acacggcggc cactgcaagc cgaagcgcga
      481 agggcaaagt tccgtatatg tcgcccgagc acatgcgtgg cgaggcggtc gatggcagga
      541 gcgatctgtt ttctctgggg gtcgcgctct tcgaagcggt ggccggatgc cggcccttcg
      601 atggagcgca cgacgtcgag acgatgcaaa agatcctgag cggagagcga ctgtcgctcg
      661 aacatgttgc gccgggagcg cccggaccgc tgcgggaggt catcgagcgt ctgatcgaag
      721 tcgatcgcga cgcgcgaaca ccgagcgcgt ctcgcttgat cgaagagctg gcggttctgt
      781 cgccaacccc ggcggtccgc cgcgagctcg ccgcaacggt cgaggaacac cgcggtggcc
      841 cgagcgcgcg cctgcacgtg cgggccaagg aagaggagcg cgacaccgag ctgggccgcg
      901 cgccgcgtcc gcatgcggac agcgcggcaa cggaatccgc tgcaacgcag aaaaccaagc
      961 cccgaagtaa gaggcgagcg ctgatcctta gcgcggccat gctgacgagc ctggccgcga
     1021 tgattgcggg aacgcagtgg ctagggtcag tgcctgtgtc agtgccagcg ccagcgccag
     1081 cgccagtgtc agtgcccgcg tcagtcccag cgccagtgcc agtgccagtg ccagcgccag
     1141 tgcccgcgtc agtcccagcg ccagtgtcag cgccagtgcc cgagccagcg ccacgccctt
     1201 ccaaaggatg gctcaacgtc gtcgttcaac cctggggcaa cgtttggatc gacggcaact
     1261 ggatgggacg agcaccggtg aaagcacgcc ttcccaaggg tcggcacctg gtagaggccg
     1321 gtcgcaagcg cccgatccaa aagcgggtca tacgagtgct cgccggctcc cgaaaagaag
     1381 tcgagttcag tctgccagac taaacgctat tcgacgacga tcgtcccgca ggggaactcg
     1441 gcggtgaacg tgacttcgcc acccttgagc gtatcgcagg cgttccccaa gagctcgatg
     1501 gtctgatatg gcggctcgtt cttcacttgc cagtcggtcg ggcagtccaa gagcacaccg
     1561 ttgagccgaa cctcgccctc gtcgcaagcc tgggccagcg tgtcggtgtt gaacggtttg
     1621 tcgatctcga tttcacacga gatgctgtca ctgatgatct gctggaatgc ctctgcgaga
     1681 agctctgcgg tcgtcgcctt ccagaacggc gcggggttac cgctctcgtt ggcaggcttg
     1741 ccgaccccca cattcgccat ctcctgaaga tgcgcgtcgc tgacctcgtt gccaacgctc
     1801 aaaatgaaga catcgagtcc ggcggcatgc gacgccgtgg cggcagcgac ggcctcggct
     1861 tgaccgtttt gaggattggg ctgctcgcag gtgtctggct caccatccgt ggcgagcacg
     1921 atgatcgtgg gcccttccgc cggcggcgga tcggactgga tgatactcac gagcgcgtcg
     1981 atgctgtcgc cggtgggcgt atcgccacct gcgttgctcg ggtagttgct cggatagatg
     2041 ttgttgttgc tgatggcgct agagttgttg agatcgaaat cgacccttgg actgacagcg
     2101 aaaggatcgc cggccacggg aagtcgaggg caaggaagat tggagtttcc gttgtcggat
     2161 gtgtaggtgc tcaagccgaa gcgcacgatg gaatcgagat ccgcgacgat ttcggtaatc
     2221 gccccatggg cagcggtcca ccgctcttgt ccagcaaagt tcgaggtcat cgacccactc
     2281 tggtcgacca agaacatcac gttcggaatg cttcgcgacg gcgtcacctc gaccgactga
     2341 caaccgatgc tgttccccgt gccccccgtg ccgcccgtac cgacgtcggg aacgcaacga
     2401 ccgcgcgtgg tgttgcaggt cgagcccgca ccgcagcctt cgttggccgt gcagttcgcg
     2461 acgcacttcg tgctgttctc gcagtaactc gccgcacact ccgaatctga gacgcaggac
     2521 gtgtcgcagt ccgcaggagg attgctgagc gtcgagcagt cgagaagcac gctcccgctg
     2581 ctgccaccac tgccgccttt ggggggtcct ccggtgccgt cgccgccgca cgcggcgagc
     2641 atcccgaggc cacccaagcc cacgaataca atcgaaagcc aattacgcat tacgaactcc
     2701 caaacatgac aaagccaact atgtcaacat tctacactgg ctctgtacgt cccgcaaatg
     2761 ccaagaggcc aatggcatag acgattatct attagtttcg atatgttatg gagattcacg
     2821 ctccggccct cacgagctct a
//
LOCUS       DASRZF010000178         3281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1496100, whole genome shotgun sequence.
ACCESSION   DASRZF010000178 DASRZF010000000
VERSION     DASRZF010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1496100"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..909)
                     /locus_tag="VFG22_07975"
     CDS             complement(94..909)
                     /locus_tag="VFG22_07975"
                     /inference="COORDINATES: protein motif:HMM:NF014510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HET6416221.1"
                     /translation="MKAGGHLSEASESQILVASAFELARNLGIPRILVLADLITDRRT
                     VEQARQNETVIWLTHGGERESYPEIRGGDACIALPPTALGRLDQVKLGLTLAVLQRHV
                     DVDTSVVCLTGVAGSKRLDMLAIINASRDFPCFRGREGKPVGAIFVLGDPNDIEPHTR
                     QLIFNPCKGHAHSVRNIHNPEFFETLRELTALDGAFLVDERGVVARAGAYLDAPASKK
                     IRVRRGLGARHVAAAAMTAHTDALSLVISESSGNITVFARGAALLELEKPPPS"
     gene            complement(1040..2230)
                     /locus_tag="VFG22_07980"
     CDS             complement(1040..2230)
                     /locus_tag="VFG22_07980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HET6416222.1"
                     /translation="MRLAILSTAPRCYSTRRLKQAAIERGHDVKVLNTLRFAIELSHG
                     NPNLHFRSKLLSRYDAVLPRIGASITYFGTAVVRQFEQMDVYTPNTSNGITNSRDKLR
                     SLQILSRHDIGIPRTAFVRDRKDVLPAIERLGGAPVIIKILEGTQGVGVILADNTKVA
                     EAIIETLQSTKQNVLIQAFVKESKGRDIRAFVVGGRVVAAMRRVAQGDEFRSNVHRGG
                     KAEPVTLDSEFQETAIRAAQIMGLRVAGVDMLESASGPQIMEVNSSPGLEGIEGATGL
                     DIAGAVIDHIGNQVAFPELDVRQRLTVSTGYGVADLLIPAGAELVGKTILQSGLRDKD
                     IVVLNLHRGTSVISNPKGTRILEAGDKLLCYGKLDSMRSMIPVSRTSARKKVRRLDPS
                     SDLG"
     gene            complement(2717..>3281)
                     /locus_tag="VFG22_07985"
     CDS             complement(2717..>3281)
                     /locus_tag="VFG22_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416223.1"
                     /translation="LQYWPIEKVMLALDAMFYGPVRSGQRAKSDSSVPIGGLFYDNDT
                     ARRAIGNVAIGGDFFITKKVMIETGFFTDFSSAINIPANPDRIYNPQINRYAGTVSIG
                     LDVGGVSLAVGATFLYGKGPATGVFIDRDNLATGYVRTEASSRSVFLHVTGATRAVSS
                     LTDKTAKGIKSYREKKHSEDKEKPSED"
BASE COUNT          597 a          998 c         1022 g          664 t
ORIGIN      
        1 agttcgcatc gctggggtcg ccatctttgc ccggcttcgg ctaggccctt tctttgcaag
       61 atgtgtcctc gaatcgaggg ccgcgcttat cggtcagcta ggcggaggct tctccagctc
      121 gagcagcgcc gccccccggg cgaagacggt gatgttcccc gacgactccg agatgacaag
      181 cgacagcgcg tcggtgtgcg ccgtcattgc ggcagcggcg acgtgacgtg ctccaagccc
      241 ccggcggaca cggatcttct tgctggccgg agcgtccaga taggccccag cccgagccac
      301 cacgccgcgt tcgtcgacca ggaatgcgcc gtcgagagcg gtcaactcgc ggagcgtctc
      361 gaaaaactcc gggttgtgga tgttgcggac ggagtgggca tgtcccttac agggattgaa
      421 gatcagctgg cgcgtgtgcg gttcgatatc gttggggtcg ccgaggacga aaatggcgcc
      481 gaccggtttg ccctcgcggc cccgaaaaca cgggaagtca cggctcgcgt tgatgattgc
      541 cagcatgtcg aggcgcttcg acccagcaac gcccgtcagg cacacgacgc tggtgtccac
      601 gtcgacatgc cgctgcagta ccgccagtgt caggcccagt ttgacttggt ccaggcgacc
      661 cagtgccgtg ggtggcaatg cgatgcatgc atcgccccct cggatctccg gatagctttc
      721 gcgctcaccg ccgtgcgtca accagatgac agtctcgttc tggcgtgctt gctcaaccgt
      781 ccggcggtcg gtaatcaagt cggcgaggac cagaatacgc gggatcccca agttgcgggc
      841 gagctcgaaa gccgacgcga ctaggatttg ggactctgac gcttcggaga ggtggcctcc
      901 ggccttcatt cgaggtttcg acttcggtcg agcttcaagc atagcgtttc acgatactcg
      961 cagttcgtcg gccgatccat cgtcgatatc atccgcggat tgcaaggtgg tcggaacgcg
     1021 ccgaatcgct cccgcaggct caaccgaggt ccgacgacgg atcgagtctc cggacctttt
     1081 tgcgcgccga ggttcggctc accgggatca tcgaacgcat cgaatcgagc ttcccgtaac
     1141 agagtagctt gtcgcctgcc tcgaggatcc gggtgccttt ggggttggag atgaccgatg
     1201 tgccgcggtg caggttgagc acgacgatgt ccttgtcgcg caggcccgac tggagaatcg
     1261 ttttgccgac gagctccgcg cccgcgggaa tcagcaggtc agccacgccg tagccggtcg
     1321 aaacggtaag ccgctgtcga acatcgagct cggggaacgc gacctgattg ccgatgtggt
     1381 cgatcactgc tccggcaatg tcgagtccgg tggcgccctc gatgccctcg agcccgggcg
     1441 aggagttgac ctccatgatt tgtgggccgc tcgccgattc gagcatgtct accccggcca
     1501 cgcgcagtcc catgatctga gctgcccgga tggctgtctc ctggaattcg gaatcgagcg
     1561 tcaccggctc ggccttccct cctcgatgca cgttgctgcg gaactcgtct ccctgggcga
     1621 cgcgccgcat ggcggcgacc acgcgtccac ccaccacgaa cgcgcgaatg tcccgacctt
     1681 tgctctcctt gacgaacgcc tggatcagca cgttctgttt ggtgctctgc agcgtctcga
     1741 tgatcgcctc ggcaaccttc gtgttgtcgg caaggatgac gcccacgccc tgggttccct
     1801 cgaggatttt gatgatcaca ggtgcaccgc ccagccgttc gatggccggc aggacgtcct
     1861 tgcgatcacg cacgaacgcc gtgcgcggga taccgatgtc gtgacgactg aggatctgca
     1921 gcgagcgcag cttgtcgcgc gagttcgtaa tgccgttcga agtgttcggg gtgtagacgt
     1981 ccatctgctc gaactggcgg accacggcgg tgccgaagta ggttatcgag gcgccgatcc
     2041 tagggagcac cgcatcgtag cgactcagca acttcgaacg aaaatgcaga tttggatttc
     2101 cgtggctgag ctcgatggcg aagcgaagcg tgttgagcac cttgacgtcg tgcccccgtt
     2161 cgatggcggc ttgcttgagc cgtcgcgtac tgtagcaacg gggagcggtc gataggatgg
     2221 cgagtctcat gttctctcct tcgaaaatcc gcgcgggacg cgtgttctcg ggcttggtgc
     2281 gggtgcgaaa aacgtcggca cagcgctgat cggcacgccg gatcggttcg gcacctggaa
     2341 cataccccga ggactcgctc ggtgataggc gcgtcatccg caagcaactg ggtcactgct
     2401 tctcgacggt tcgacaggtt ctttcaactg acggccacgc ctcgggattc tcgaggacgc
     2461 gaggggtctc cgaatcggcg gggtttcagg cccaatatcg actttctcgg attccaagca
     2521 gggcgttcca gtcgcacacg aagccaagta ccgagttcca acttcacatc gatggggtca
     2581 ccaaaccgga gcggatctcc gcacctacaa cggcgcagcc caaagcatcg cagaggggaa
     2641 gtaggtcact cttgcccgcc tcgagcgcgt tgcgcgtgct gtgatgctgg ccacaggacg
     2701 tagtgcgagc gcccggctaa tcttcgctcg gcttctcttt atcttcgcta tgctttttct
     2761 cgcgatacga cttgatgccc ttggccgtct tgtcagtgag actggaaacc gcccgagtag
     2821 cgcccgtgac gtgtaggaag acgctacgag agctcgcttc tgtgcggacg taacctgttg
     2881 ccagattgtc tcgatcgatg aagacaccgg tcgccggtcc cttgccgtag aggaaggtgg
     2941 ctcccacggc aagcgagaca ccccctacgt cgagacctat cgagacagtg cccgcgtagc
     3001 gattgatctg cgggttgtag atccgatctg gattcgcggg aatattgatc gctgaagaga
     3061 aatcggtgaa gaagccggtt tcgatcatga ccttcttggt gatgaagaaa tctccgccga
     3121 tcgcgacgtt cccaatggca cggcgcgcgg tatcgttgtc gtagaacagg cccccgatcg
     3181 gtaccgatga gtcgctcttc gcgcgctgcc ctgagcgcac cgggccgtaa aacatcgcgt
     3241 ccaaggccaa catgactttc tcaataggcc aatactggag a
//
LOCUS       DASRZF010000179        10578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_810214, whole genome shotgun sequence.
ACCESSION   DASRZF010000179 DASRZF010000000
VERSION     DASRZF010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 10578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10578
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810214"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..3865)
                     /locus_tag="VFG22_07990"
     CDS             complement(8..3865)
                     /locus_tag="VFG22_07990"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HET6416224.1"
                     /translation="MHRLLIHALIGLSLIVTTSASAQSTGNSFRDEFEGSSLGSGWTF
                     TDHFADAYPSRTADHVSLSMSGDQVSMSFRGGAEHNQWWIEHAQITRPFLGAGVYEMR
                     MDTGIMSNEQFGIVFQGENPATFMMFMMYGNGSVRGYIERFVSVDGAIYRHTIPGSFR
                     PSIPLPSAGPYYMRVTVSDDVPTQRVWKFEYSLDGTTWHLSREEVLEDFGLGENIGAI
                     TEVGLFVGNHPSALPGFDGRFAYFDYKPFGQLPPPAPSNLIALADDGSVELIWNAVFG
                     SEGYNVYRDDGAGPTLLTTTTDTSHLDTTVANGTQYTYAITAFQGALESSAATVVALP
                     QGVVPPVDPPADFPVEGMLLWLDADTALAETGAGNPATLWRDGSGWANDAAAGSIEAP
                     TVIPAAINGRAALQFDGIDDHLRLPAGFEDFRDGMTLFIVAQPTALQPGFKLVALGNG
                     AGSDMIVLGRNGGTAGLQYFTSDANDYVKWFGSADALVPGEAGLITVRQGAGIENAVV
                     STSVFKNGEPVGSGNVYVPPVIARSENLIGRSFWNEGMFEGKLAEVLIYDRELADAEA
                     AIVQAYLDRKYALGIDPSNPPPDPDPGPDPIVLDAPANLVATAGDANVSLSWDAVAGA
                     TGYRLWRSEGSGSTFNSFYEITDTSHTDTGLVNGQAYTYVVTAFDVTSESADSSPVTA
                     TPVANNPDPPSSGQLPAAGLVLLLDAEQAIADFGAGAEVTSWQDLSGTGSHAEAFPGE
                     APVVVANAINGRPALSFDGVDDYFSVTSGLDDFTSGLTLYVVARTTVLQQGFKLLALG
                     NGAGNDMIVLGRAGGSSGLQYFTSDDRGAVGWFNTGSALTAGDIALYSVRQGGGGTGD
                     PVTAVVERDLDVVGSGRVFVPTVTARTGSLIGRSFWNEGMFQGQLAEVVLYDRSLSDA
                     ERAEVEAYLDDKYSLGLIQTVPPDPGPDPEPDPVPPNAPAILDAVGGDAVVTLSWSAV
                     TGASGYRLWRANDGSTFGLLYEGPATSFIDAPLTNGQAYYYVVTAFDSAGESADSVQA
                     TATPVAIVPPDPDPEPDPPPAALPSSGLRLLLDAGSAAADFGLGVPVTSWRDQSPDGS
                     DAWSSVATAPVLVADAIGGQPALRFDGIDDHFNLPTGFEDFTAGVSLFVVMRPTVLQQ
                     GFKILALGNGANQDMLVLGRAGSQNGLQYFTNNEFGNVTWFNSAPVLSEGSAALFAIE
                     HEGSTARVVHNGVVITESAVDLPSVSPRGVNYIGRSYWREGYLQGDLAEIILYDRTLT
                     DDERLSVEAYLDAKYSLGIGATP"
     gene            complement(4303..4536)
                     /locus_tag="VFG22_07995"
     CDS             complement(4303..4536)
                     /locus_tag="VFG22_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416225.1"
                     /translation="MERVTDWPTSTYVEIDHPWISNDETPLYRWTFPEASTDEELHAC
                     LAAPQGVAGTPEEIMVAGGHGGFLTTCANWFGL"
     gene            4648..5277
                     /locus_tag="VFG22_08000"
     CDS             4648..5277
                     /locus_tag="VFG22_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416226.1"
                     /translation="MGEIDWSPTIGDPTLMGWLTVLAYFVTAWLCLRAFNTEKRGPKR
                     PYRQTIPALLRVLRKSWPEPPILARRAALWLFLAIVFVCLGINKQLDLQSLLTQVGRT
                     LAHQQGWYDQRRLAQAILVGVVLIAGVAALATLGLLVRGQLSDFRLPLAGLTFIVVFV
                     VIRATSFYHVDRLIGFELRGVRMNWVLELTGIGIVAVGAIRRQRRGLEP"
     gene            complement(5321..6724)
                     /gene="cls"
                     /locus_tag="VFG22_08005"
     CDS             complement(5321..6724)
                     /gene="cls"
                     /locus_tag="VFG22_08005"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HET6416227.1"
                     /translation="MSSTLAIVLLVSELFGVLLAVDAVMRPRSPQGAIAWSVALVSIP
                     TVAIPVYLVFGRTQFRGYAEALRDKERQLDERIPDWYRRMAAFAAQPKAGLERIEAVA
                     RRLTNVPFTAGNCVELLVDAEATYGSMLDAIGRAQTYVLVQFYIVRDDRSGRALRDAL
                     IERARDGVRVCFLYDEIGSIKLPASFLDEMRRASIRVSGFRTTQGFRNRFQINFRNHR
                     KLLVVDGRTGFIGGLNLGDEYRDFRDTHLRIEGPAVQQIQLTIRKDWYWATREIIDFA
                     DQPIVATEPGQAVTIVNTGPADPVPKCSILFSELVAGARSRVWIASPYFVPDDVMTRA
                     LQSAAKRGVDVRIVLPDEPDQRFVELASLTYYTEMMGSGVRLFRYKGRFMHHKVILVD
                     DDLAAVGTVNLDYRSLYLNFEETALVADQQFARRVGAMLDDDLAHCDEVAPGYLENQP
                     FHIRIQARVARLASPLL"
     gene            complement(6794..7525)
                     /locus_tag="VFG22_08010"
     CDS             complement(6794..7525)
                     /locus_tag="VFG22_08010"
                     /inference="COORDINATES: protein motif:HMM:NF011765.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HET6416228.1"
                     /translation="MRISSGLLGAALGAWLVLCALVGCQQKSAEMPAPRVETVLTSEQ
                     QQKLTPAQVIEALQEGNQRFVSGDLTVRDHSAQVRAAVGGQFPKAAILSCLDSRIPVE
                     DVFDRGIGDVFVARVAGNFENTDILGSLEFATKVAGAKVVLVLGHEHCGAVKAAIDGV
                     ELGNITPMLSNIQPAVGHFSDYAGDKSSKNEDFVEMVSEENVRMTMKDIRSRSPIIKE
                     LEDAGSLKIVGGIYDMDTGQVNLIE"
     gene            8009..8461
                     /locus_tag="VFG22_08015"
     CDS             8009..8461
                     /locus_tag="VFG22_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416229.1"
                     /translation="MTKALWIGSLALLAAQLGGCKLKKHLDLVTANEAEATFVLQYEH
                     GFEHYLVEWDKAEKEALERCTAWGYSAVEFSDSGTVECIEKHQRNVTGARPPSTSAEP
                     GMGTEAAERERMRRSLGTVGKSVSRTPPAGAEYGCIRWRVTYTGHCFD"
     gene            complement(9165..9713)
                     /locus_tag="VFG22_08020"
     CDS             complement(9165..9713)
                     /locus_tag="VFG22_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416230.1"
                     /translation="MPLVGCGDNNGGGGSGGTGGGGGSGAAGGGEIGDQFPAIDTLVL
                     TLNRVYDDGDQRGDEQYTFQVAELTVTHEWLRSSVPPESLPPATREAMDRLVSVVQGV
                     SYRINEDCGMWAVDYVPFPPMLSVVHAQDRLVLSVSDSKCADNDHSFVGPVMSCTDFD
                     KAFAAIREIVPEVESETCADYW"
     gene            complement(9770..>10578)
                     /locus_tag="VFG22_08025"
     CDS             complement(9770..>10578)
                     /locus_tag="VFG22_08025"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HET6416231.1"
                     /translation="GAQTVVTEFEIVIDNDVILDGEGNLTVDGDQHHRMFWVRNGVVA
                     ELRGVSVRRGRALGEVTEDREGGGIFNEGTLTLTHSTVSGNGTEYQGGGIFNEGTLTL
                     THSTVSENSAIVDWADAEGGGIYKLTGTLALTDSTVSGNRATSEGGGIYNFLGTLTLR
                     SSLIDGECAGTIASAGHNIESPGDTCSFDQPTDQVNVSADDLNLGPLQDNGGPTQTRA
                     LGADSVAIDQIPAEDCVDADGAPLTTDQRGQPRPETGGTMCDVGAFEVQP"
BASE COUNT         2032 a         3294 c         3192 g         2060 t
ORIGIN      
        1 ctgtttccta gggggtcgcg ccgattccta gggaatactt tgcgtcaaga taggcctcga
       61 cggacaaacg ctcgtcatcg gtgagcgttc ggtcatagag gatgatctcg gcgaggtcac
      121 cttggagata gccctcccgc cagtagctcc ggccgatgta gttgacgccg cgcggtgaga
      181 cgctggggag atcgacggcg ctctcggtga tcacgacccc gttgtgcacg acccgtgcgg
      241 tggagccctc gtgctcgatc gcgaacagag cagccgagcc ctctgacagc accggcgcgc
      301 tattgaacca tgtcacattc ccgaactcgt tgttcgtgaa gtattgaagg ccgttctggc
      361 tcccggcacg accgagaacc agcatgtcct ggtttgctcc gttgccgagc gccaagatct
      421 tgaagccctg ctgaagaaca gtcgggcgca tcaccacgaa gagcgagacg cccgcggtga
      481 agtcctcgaa gccggtgggc aagttgaaat gatcgtcgat gccgtcgaag cgcagcgccg
      541 gctgtccgcc aatcgcatcg gcaacgagca cgggagcggt agccacgctg gaccaggcgt
      601 cggagccgtc cggggactgg tctcgccaag acgtcacagg cactcccaag ccgaagtcag
      661 ctgctgcgga gccagcgtcg aggagcaggc ggaggcctga gctcggaagc gccgcgggag
      721 gcgggtcggg ttctggatca gggtcgggcg gcacgatcgc aaccggcgtc gcggtggctt
      781 gcactgaatc ggcagactct ccagcggagt cgaacgcagt gaccacgtag tagtaagcct
      841 gcccgttcgt cagcggcgca tcgatgaagc tagttgccgg gccttcgtat aggagcccga
      901 aggtcgaacc atcgttggca cgccacaagc ggtagccact ggcacccgtg accgcgctcc
      961 aggaaagcgt gacgaccgca tcacccccaa cagcgtcaag gatagccgga gcgttcggtg
     1021 gaaccggatc tggctccgga tcggggccgg gatcgggcgg cacggtctgg atcaagccga
     1081 gcgagtactt gtcgtcgagg taggcttcca cttcggcgcg ctcggcgtcg ctcaagctgc
     1141 gatcgtagag aacgacctcc gcaagttgcc cctggaacat tccttcgttc cagaagctcc
     1201 gcccaatgag gctgccagtg cgggccgtca cggtgggcac gaagaccctg ccgctgccaa
     1261 cgacatcgag atcgcgctcg accaccgcgg tgactggatc gccagtgcca ccacctccct
     1321 gccggacgga gtacagcgcg atgtcgcccg ctgtcagcgc ggatccggtg ttgaaccagc
     1381 caacggcccc gcggtcatca ctcgtgaagt actgaagacc agacgaccct ccggcgcggc
     1441 cgagtacgat catgtcgttc ccggcaccat tgccgagggc cagtagcttg aagccttgct
     1501 gaagaacggt cgttcgcgcg accacgtaga gggtgagacc cgaggtgaaa tcgtcgagcc
     1561 cactggtcac actgaagtag tcgtcgacac catcgaagga aagagcagga cgaccgttga
     1621 tcgcgttggc aaccaccacg ggcgcttcgc cggggaacgc ctcggcatgg gagcctgtac
     1681 cgctgagatc ctgccacgac gtgacctcgg cgcctgcacc aaaatcagcg atggcctgct
     1741 ccgcgtcgag gagcaaaacg agacccgcag cagggagctg cccggagctc ggcggatcag
     1801 gattgttggc gacgggcgtc gcggtaaccg gcgacgagtc cgcggactcg ctggtcacat
     1861 cgaaggcggt caccacgtag gtgtacgcct gaccattgac caagccggta tcggtgtggc
     1921 tggtatcggt gatctcgtaa aagctattga acgtgctccc gctgccttcc gagcgccaca
     1981 agcgatagcc cgtcgcgcca gcaaccgcgt cccatgagag cgaaacgttg gcgtcgccgg
     2041 ctgttgccac gaggttcgcc ggcgcatcga gtactatcgg gtccggaccg ggatccggat
     2101 cgggcggcgg attgctcggg tcgatcccga gggcgtactt ccggtcgaga taggcctgca
     2161 cgatggcggc ctcggcgtca gcgagctcgc gatcgtagat cagaacctct gccaatttgc
     2221 cctcgaacat gccttcattc cagaagctcc tgccgattag attctcggag cgcgcgatga
     2281 ctggaggcac atacacgttg ccactgccca ccggctcgcc attcttgaat acgctcgtcg
     2341 acacgaccgc gttctcgatg ccagcgccct gccggacggt gatgagaccg gcctccccag
     2401 gaacgagagc gtcggcgctg ccaaaccatt tcacatagtc atttgcgtcg ctcgtgaagt
     2461 actggaggcc cgctgtgcct ccgttacggc cgaggacgat catgtcggag cccgcaccgt
     2521 ttcctagcgc cacgagcttg aatccgggtt gcaacgccgt cggctgagcg acgatgaaca
     2581 gcgtcatgcc gtccctgaag tcttcgaacc cggcgggcag gcgaaggtga tcgtcgatgc
     2641 cgtcgaactg aagagcggcg cggccgttga tggctgcggg gatgacggtg ggtgcttcga
     2701 tggatcccgc cgcggcgtcg tttgcccacc ccgaaccgtc acgccaaagc gtagccgggt
     2761 tgcctgcgcc cgtttcggct agcgccgtgt cggcatcgag ccagagaagc atgccttcga
     2821 cgggaaagtc tgcgggtgga tcgacgggtg gcacgacacc ttgcggcagt gcaacgaccg
     2881 tggcggcgga ggattcgagt gctccctgga acgccgtgat ggcataggtg tactgcgtgc
     2941 cattcgcgac tgtcgtatcc aggtgcgagg tgtcggtcgt cgtggtcaac aaagtcgggc
     3001 cggcaccatc gtcgcggtag acgttgtagc cttctgaacc aaatacggcg ttccagatca
     3061 gctcgacgga tccgtcgtct gccagggcga tcaagttgct gggtgcgggt ggcggaagct
     3121 gaccgaacgg cttgtagtca aagtacgcaa atcgtccgtc aaatccggga agagccgaag
     3181 ggtgatttcc gacgaagagg ccgacttcgg taatcgctcc tatgttttcc cctagaccga
     3241 agtcttcgag gacttcttcc cgcgagaggt gccaggtcgt gccgtccagc gagtactcga
     3301 acttccagac tcgctgcgtt ggaacgtcgt cgctgacggt cactcgcatg tagtacggac
     3361 cggcgctcgg gagcggaatc gagggccgga agcttcctgg aatcgtgtga cggtagatcg
     3421 ctccatcgac ggatacgaag cgttcgatgt acccgcgtac gctaccgttg ccgtacatca
     3481 tgaacatcat gaaggtggcg gggttttcgc cttgaaagac gatgccgaac tgctcgttgc
     3541 tcatgatccc ggtgtccatg cgcatctcgt agacgcccgc acccaagaac ggacgtgtga
     3601 tctgcgcgtg ctcgatccac cattggttgt gctcggcgcc gccgcggaac gacatcgaaa
     3661 cttgatcccc gctcatggaa aggctgacgt ggtccgcggt ccgcgaagga taggcgtcag
     3721 caaagtgatc ggtgaacgtc catccagacc ctaggctcga gccttcgaac tcgtctcgaa
     3781 acgagttgcc agtcgactgc gccgaggctg acgtggttac aattaacgac aatccgatga
     3841 gggcgtggat cagcaatcgg tgcattcgaa ggcctgcaat attgacgggt tggggggtaa
     3901 acagggctga acagacgaat cgttcaggag agtgcaaagc ggaacaacgc ttcgtatatc
     3961 aaactgtaaa cgaatgtaaa cgcaaaatct gcgctatccc aagtcaatat gtgtgagatt
     4021 gcttacataa ccggcatgtt tcccagccct cggctgcgat cgaggacagc cgcgtcaaca
     4081 agagggtgag tgaaggctca agcccagtgc catgggtgca aggagcgctc gggctccgcg
     4141 agcttgggcg cgacgaagtt agccgtcact cggggagccg tcaggcgcgg accatcgatc
     4201 tgcgcaagtg gccggatcct gcagagaaac cagaaatccg ccctagtgcc gggggcggga
     4261 ttcgaccagt tgatcccacg ctctctacgc ctgtcctacc cttcacagtc caaaccaatt
     4321 agcgcaggtc gtaaggaaac cgccgtggcc cccggcgacc atgatctcct cgggggtacc
     4381 cgcgacccct tggggtgctg cgaggcacgc atgaagttcc tcgtcagtgc tcgcctctgg
     4441 gaacgtccat cgatagagcg gcgtttcgtc gttcgagatc cacggatggt cgatctcgac
     4501 gtacgtggaa gtgggccaat cggtcacgcg ttccacgacg tccttgatag taccgcttct
     4561 cgagctcgaa agccgattgc aatcgatccc ggcacgctcg acagtgccga cgtgtaccgg
     4621 gccgctctag gctgaggagg ttcaccgatg ggagagatcg attggtcccc tacgatcggc
     4681 gatcccacgc tgatgggatg gctcaccgtg cttgcctact tcgtcacggc ctggctctgt
     4741 ctccgagcct tcaacaccga gaagcgcggt cccaagcgcc cgtaccgaca aaccatccca
     4801 gccttgcttc gggttctgcg taagagctgg ccggagcctc cgattctcgc gcgtcgcgcg
     4861 gcgctctggc tgtttctggc gatcgtattc gtctgcctcg gcatcaacaa gcagctcgat
     4921 ctgcaatctc tgttgacgca agtgggcaga accttggccc accagcaggg ctggtacgat
     4981 cagcgccgcc tcgcgcaagc gatcctcgtt ggcgtcgtgc tgatcgcagg cgtggctgcc
     5041 ctcgcgacgc tcggcttgct cgtccgcggg caacttagcg atttccgtct accgctcgcg
     5101 ggtcttacat ttatcgtcgt cttcgtggtg atccgggcca cgtcgtttta ccacgtcgac
     5161 aggttgatcg gcttcgagct tcgcggtgtg cgcatgaact gggttctaga gctcacgggc
     5221 attggcatcg tcgcggttgg cgcgattcgg cgccagcgta gaggcctcga accttgaagc
     5281 gccctcagcg tccgaatcgt gtgattcaag ctgtgaaccg tcataagagc ggtgaggcga
     5341 gccgagccac gcgcgcctgg atgcggatgt ggaacggctg gttttcgagg tagccaggag
     5401 ccacctcgtc gcaatgcgct aggtcgtcgt cgagcatcgc cccgacccgt cttgcgaact
     5461 gctggtcggc aacgagggcg gtctcttcga aattcagata gagagagcgg taatccaaat
     5521 tgacggtgcc aaccgcagct aagtcgtcgt cgaccaagat gaccttgtgg tgcatgaagc
     5581 gccccttgta gcgaaacagt cgcacgccgc tgcccatcat ttccgtgtag taagtgagcg
     5641 atgcgagctc gacgaagcgc tggtccggtt cgtcgggaag cacgatgcgc acgtccactc
     5701 cgcgcttggc agcggattgc agcgcgcgtg tcatgacgtc atccgggacg aaatacgggc
     5761 tcgcgatcca gactcgcgat cgcgcgccgg cgacgagctc tgagaagaga atgctgcact
     5821 ttggaactgg atcggcggga cccgtattga cgatggtcac cgcctgacct ggctcagtcg
     5881 cgacaatggg ttggtcggca aagtcgatga tttcgcgagt cgcccagtac cagtcttttc
     5941 gaatagtcag ctgaatctgc tgaaccgccg gcccctcgat tcgcaggtgc gtgtcgcgga
     6001 agtcgcggta ttcgtcgccg aggttcaagc ccccaatgaa ccccgttcgc ccatccacga
     6061 ccagtagttt gcggtggttg cggaagttga tctgaaaccg attgcgaaag ccctgggtcg
     6121 tccgaaagcc agagacccgg atcgacgcgc gacgcatctc atcgagaaaa gaagccggta
     6181 gcttgatcga gcctatttcg tcatagagga agcacacgcg gacgccgtcc cgagcccttt
     6241 cgatgagagc atcgcgaagc gcccgtccac tcctgtcgtc tctcacgatg tagaactgga
     6301 ccagcacgta ggtctgcgcc cgtccgatcg cgtcgagcat ggatccgtag gtcgcttcgg
     6361 catcgacgag cagctccacg cagttcccag ctgtgaaggg cacgttggtc agccgtcgag
     6421 cgaccgcttc gattcgttct agccccgcct tcggctgggc cgcaaaggcc gccatccgtc
     6481 ggtaccaatc gggtatccgc tcatcgagtt gccgttcctt gtcgcgcaaa gcctcggcgt
     6541 aacctcggaa ctgcgttcgc ccgaacacca gatagacggg aatcgccacc gtcggaatag
     6601 agacgagcgc gaccgaccag gcgatggctc cctgaggcga gcgcggccgc atcacggcgt
     6661 cgactgccag tagcactcca aacagctcgg agacgagcag aacgatagcc aacgtcgagg
     6721 acatcagaac tccgggtcgg gggggtcagg gcagactagc cctaccactt cccacgcggc
     6781 ccgacagcgt ctctcattcg atgagattca cctgtccagt atccatgtcg tagatcccgc
     6841 cgacgatctt caaactgcct gcgtcttcga gctctttgat gattgggctg cgcgaccgga
     6901 tgtccttcat ggtcattcga acgttttctt cgctgaccat ctccacgaag tcctcgttct
     6961 tgctggattt gtcgcccgcg tagtcggaga agtgacccac ggcgggttgg atgttgctca
     7021 gcatcggcgt gatgttgccg agctcgactc catcgatggc ggccttgacc gccccgcagt
     7081 gttcgtgccc gagcacgaga acgaccttgg caccagccac cttcgtggcg aactcgaggc
     7141 tgccgaggat gtccgtgttc tcgaagttgc ccgcgacccg cgccacgaaa acgtctccaa
     7201 tgccccgatc gaacacatcc tcgacgggaa ttctagagtc gaggcacgac aagatcgcag
     7261 ctttggggaa ctggcctccc acggcggctc tcacttgcgc gctgtgatct cgcaccgtga
     7321 gatcgcccga gacgaagcgt tgattcccct cttgcaacgc ctcaatcact tgggcgggcg
     7381 tgagcttctg ttgttgttcg ctggtgagga cggtttccac ccgtggcgct ggcatctcag
     7441 ccgacttctg ctggcaaccc accagcgcgc acaacacgag ccaagctccc agtgcggccc
     7501 ctagtaaacc tgaactgatt ctcatcgcgc ctcctcggtg tccaccccgc gcgggcggta
     7561 gaacaggcgc catgttaggt cgtcaggata tctctcacaa gggcgcacgc catcaggtca
     7621 tgtcatgtcg cccggcttcc ttgatcgagt ggagggaacc atggcggcaa tggacgtgtg
     7681 accgtcatga agactgatag cccacctccg cccggaatgt tccggtagtc gagggtctgc
     7741 tgttcccaga ttcgattttc gttgaggcgt gcacctgacc agtgacgaac aagccggccc
     7801 aaatgccgaa ctcctcgtcg gtctgtgcgc agcccccgtg ccgcaccaaa gcgagcgccc
     7861 cgatacaaag gactagaaga aaccgttggc gcatgtgcat gcccacaatc tgatgtcctg
     7921 ggggcgtttg tctttgaccg ctaggaacac cccgacgggc gccgcttcac acctgcacta
     7981 gcggatgata cagtgcctga acgttcctat gaccaaagcc ctttggatcg gttcgcttgc
     8041 tttgctcgcc gcacaactcg gcggatgcaa gctcaaaaag catctagacc tcgtaactgc
     8101 caacgaggcc gaggcgacct ttgttctgca gtatgagcac ggtttcgagc actatctggt
     8161 cgaatgggac aaggccgaaa aggaagccct ggagcggtgc accgcgtggg ggtattcagc
     8221 cgtggagttt tccgactcgg gcaccgtcga atgcatcgag aaacatcagc gcaacgtcac
     8281 cggcgctcgc ccccctagca cctccgcgga gcccggcatg ggcaccgagg cggcggagcg
     8341 agagagaatg cgtcgcagtc tcggtaccgt gggcaagagc gtcagtcgca cgcccccggc
     8401 tggggctgaa tacgggtgta ttcgttggcg cgtgacgtat accggtcatt gcttcgacta
     8461 gccgaacgag aagatctaac cgacatcggc ccaaacgaag cgagcatcga aagattgcct
     8521 tctggtttcg tgctgtttcg ttctctcttt tcgcttcagt cgccaacttg tttttcgtgc
     8581 catggggaac gctccttggc gcgaatgagg acgtaatgga aacccaacct aatgccgggg
     8641 gcgggattcg aactcgatga gccccacatc cgcaaacctc tttcacacat agcaattcca
     8701 accgattatc aataatcgct gggacttgcc cgcatcgccg tgttcacggt tcgtcacttg
     8761 atttcacgtc gccatgtcaa cgtcaggtca acgagcgcaa gcaggacgag cgagtttact
     8821 accctgccgc tagaccgtcc gcggagacga tctcgcccca ggtcgcgact gagggagaac
     8881 aacgctgtgg gttgcggggg ccagatttga actgacgacc ttcgggttat gaggacgcgc
     8941 tatgtgcctt gctcgacttg tgtagagcgt ttctagcacc tctttcgatc acggcgactt
     9001 gccatgccta tcggacttgg cctgttcgct ggtcctgtgg cccccaaatg gccctagtct
     9061 ttcttttcct acggctgtac ctcaaatgcg cccacgtcgc acatcgtgcc gccgggtggc
     9121 ggtgtgccaa cgctgcgacg acgctttggg cgagtgaagg ccgattacca gtagtcggcg
     9181 cacgtttccg actcgacctc gggcacgatc tcgcgaatgg cagcgaaggc cttgtcgaaa
     9241 tcggtgcagc tcataaccgg tcccacaaaa ctgtggtcgt tgtcagcaca tttgctgtcg
     9301 ctcacactca ggaccaaccg gtcttgtgca tgcacgacag acagcatcgg cggaaatggt
     9361 acgtaatcca ccgcccacat cccgcagtcc tcattgatgc ggtaggaaac gccctgtacg
     9421 acactcacga gccggtccat cgcttcacgg gtcgcagggg gaagcgactc gggtggaacg
     9481 gaactccgca accactcgtg cgtgaccgtc agctcggcaa cctgaaacgt gtactgctcg
     9541 tcaccccgct ggtcgccgtc gtcgtacacc ctgttgagtg tgagcaccaa cgtgtcgatc
     9601 gcaggaaact gatccccgat ttcaccgccg cccgccgctc ccgaaccgcc gccccctccc
     9661 gtgccaccac tgccaccgcc accattgttg tcaccgcacc ctactagagg catggcacct
     9721 aacgcgcaga cacatacaaa tcccactacg taacgcatga cccacctccc tacggctgca
     9781 cctcaaacgc gccaacgtcg cacatggtgc cgcccgtctc tggtcgtggc tgtccacgct
     9841 ggtccgtcgt gagcggtgcg ccgtctgcgt ccacgcagtc ctcggcagga atctggtcga
     9901 tggctacgct gtccgcgcca agcgcacgcg tctgcgtcgg cccgccgttg tcttgcagcg
     9961 gtccgaggtt caggtcgtcg gcgctgacgt tgacctggtc ggtgggttgg tcgaagctgc
    10021 aggtgtcgcc gggagattcg atgttgtggc ccgccgaagc aatcgtgccc gcgcactcgc
    10081 catcgattag gctgctcctc agcgtcagcg ttcccaagaa gttgtagatg ccgccgccct
    10141 cgctcgtcgc cctattgccc gacaccgtgc tgtcagtcag cgccagcgtg cccgtgagct
    10201 tgtagatgcc cccgccttcg gcgtcggccc agtctacaat agcgctattc tccgacaccg
    10261 tgctgtgggt cagcgtcagc gttccctcgt tgaagatgcc gccgccctgg tactccgtgc
    10321 cattccctga caccgtgctg tgggtcagcg tcagcgttcc ttcgttgaag atgccgccgc
    10381 cttctctgtc ctcagtaacc tcacccaacg ccctcccgcg gcgaaccgac accccgcgga
    10441 gctccgccac tacgccgttt cgaacccaaa acatgcggtg gtgttgatcg ccatcgaccg
    10501 tgaggttacc ttcgccgtcg agaatgacgt cgttgtcgat gacgatttcg aattcggtca
    10561 ccaccgtctg cgctccat
//
LOCUS       DASRZF010000180        13034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4250062, whole genome shotgun sequence.
ACCESSION   DASRZF010000180 DASRZF010000000
VERSION     DASRZF010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 13034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13034
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4250062"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VFG22_08030"
     CDS             complement(<1..227)
                     /locus_tag="VFG22_08030"
                     /inference="COORDINATES: protein motif:HMM:NF017046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HET6416232.1"
                     /translation="MSEFAARRQQLLDAMGDGVAVFPSAPLALRNHDVEHPYRQDSDL
                     HYLSGFDEPESLLLLTNQHADHRVVLFVRPR"
     gene            complement(244..1689)
                     /locus_tag="VFG22_08035"
     CDS             complement(244..1689)
                     /locus_tag="VFG22_08035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005416 - amino acid:monoatomic cation
                     symporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid carrier protein"
                     /protein_id="HET6416233.1"
                     /translation="MLQSLDGGFGAHVVAPIAKVLFFDLIFWDNGSDHDLKLPFIVFW
                     LIAGATFFTLRFGFINLRAFRHAWVVTTGHYDDPDDPGEVTHFQALSSALSATVGLGN
                     IAGVAVAVAVGGPGAIFWMIVAGFLGMSSKFTECTLGQMYREVADDGHILGGPMRYLD
                     RGLGELGLPRLGRVLAVVFAVMCIGGSFGGGNMFQANQSYAQVADIMPFFAGGWGSLA
                     YGVTLSFLVGIVIIGGIRRIGQVAGFIVPVMCGIYLVAGLFIIITNFDQILPSLQKIV
                     GEAFSPEAGFGGMLGVLVTGFRRAAFSNEAGVGSASIAHSAAATDEPVREGIVALLEP
                     FIDTIVVCTMTGLVVVITGAYEGGGEEGVLMTSKAFATVLPWFPNVLSLAVFLFAFST
                     MISWSYYGERCWTFLFGAQFSMIYKVLFLGFIVLGSVLKLGNVIDFSDLMILGMSFPN
                     ILGAFLLSGKVSDALKSYWSRYKSGEMQPRS"
     gene            2316..2675
                     /locus_tag="VFG22_08040"
     CDS             2316..2675
                     /locus_tag="VFG22_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HET6416234.1"
                     /translation="MFSAYLPVFLMLGVAVLVAVGVFALTSVLGPKNMTAEKAIPYES
                     GSETTGASHIRLSIKFYLTAILFVVFDIEAVFLYPWASLFRDLGWVGFIEMFSFLVVL
                     GVTLVYAWRKGALNWES"
     gene            2663..3208
                     /gene="nuoB"
                     /locus_tag="VFG22_08045"
     CDS             2663..3208
                     /gene="nuoB"
                     /locus_tag="VFG22_08045"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HET6416235.1"
                     /translation="MGELKVIDGGGEVAVTTRLSDLLQWARKWSLFQYPFVTACCGME
                     FMATAAPRYDIARFGAEAPRFSPRQSDVLWVVGTISQRQGPAVRRIWEQMADPKWVIA
                     FGTCASCGGFYDNYTTIPGVDKVIPVDVYIPGCPPRPEAVLDALMLLQEKIQANDRKP
                     IVVPPRLVPERARGRSGEGQS"
     gene            3205..3933
                     /locus_tag="VFG22_08050"
     CDS             3205..3933
                     /locus_tag="VFG22_08050"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HET6416236.1"
                     /translation="MSQAVLDRLSTQFGERVLGTSDAFGDYELQIALKDWVEVAQFMK
                     ADEQLRMDHFIDLTAIDYPERESARFDLTLTTRSSSNCDRIRIRTYVKEDEEPGTLSQ
                     VWAGANWAEREVWDMFGIRFKGHPDMRRILLYEEFVGHPLRKDYPIDKAQPLVPYREV
                     DDIEKLPPFGIEEGQPFARIDWESRLAGGDQQVSPSIGVQQGQRRTLSDSEVAEHLHA
                     RIAARMAKEADTASSDAGEKGSGE"
     gene            3934..5151
                     /locus_tag="VFG22_08055"
     CDS             3934..5151
                     /locus_tag="VFG22_08055"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HET6416237.1"
                     /translation="MEPADELSNDATALELHSDPMRLNMGPSHPAMHGTIRMVVDLDG
                     ENLVNLDVQPGYLHRGFEKSCERGTWAQVFPYVDRLNYVSPMLNNVGWSLAVEKLLQI
                     EVPERCQYYRVILGELARIADHFTCNGASAMELGAFTPFLWLLKVRDWVWDILERETG
                     ARMTHSFGRIGGMAAPPTINFKDDVLRLIPEVHKVVKETEIMLLRNRIFLDRMQGVGV
                     LTKEQALSYGVTGPVARSTGIPYDVRKDHPYLVYDRFDFDVPVGEDGDNWDRFMVRLE
                     EIRQSVRIIEQAIEQMPDEGPVNVDDPRIVYPAKGDVYTTIEATIAHFKLVMEGLRTP
                     KGEVYSFTEGGNGELGFHIVSDGSGTPYRVRVRPPCWYNLAAAREMLLGGMIADIIPT
                     FGSVNMIGGECDR"
     gene            5155..6729
                     /locus_tag="VFG22_08060"
     CDS             5155..6729
                     /locus_tag="VFG22_08060"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET6416238.1"
                     /translation="MPKFVLDGKEIAFEPGETVMQAAWREGIEIPHYCWHPGLSIAAN
                     CRMCLVQIESGRQMAMPQLAFDEAKGEYVPATKPKLQPACQLAAAEDMVISSTNDEVK
                     NAQASVQEFLLLNHPVDCPICDQAGECKLQDYYYEHQGTLKRKLTEPVHKPKGVRFGP
                     TIVYDAERCIMCTRCIRVCDEVAKDHVLDMRERGNKNEIILAPGRELDHKYTLMTEHV
                     CPVGALTSQDFRFKARVWFLKSRPSVCTGCATGCNSHADFDPRDGKIYRLRPRDNEAV
                     NKFWMCDDGMMSYHRHDTDRVLMPTVGRGDERTPIGEHEALELAAEQLRGVDPSKLAV
                     VLSAQSSTEDNLALAKLAATLGAGQLYLGALGGWEGDDILRSADNNPNRAGATQAASG
                     APLKSVAELVADVQGGTVQGVLALGWATAEKLEELAPLVALNAVISLTSHVGPLPAVA
                     SITIPVADTFEIDGSFVNEKGIAQSFGPVLPLPAGVEPAWKTVSDLARVLGAKLGFDD
                     LKGLRQGLQDAAEAAQ"
     gene            6726..8141
                     /locus_tag="VFG22_08065"
     CDS             6726..8141
                     /locus_tag="VFG22_08065"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HET6416239.1"
                     /translation="MSTTFLVVSSMVKVLVILLITVGAFAPILIWAERRQSAMIQDRL
                     GPSRAAIVLPASALAFIDRARLPIWRLAALTGIVAIVALVTLTFVAIKGSNPLPVSTS
                     TLTTSLVGFGVASVLLRAGYGVHSMIVRNGGKIMLIGLLHPLADAIKFIFKEDFVPPK
                     ADKLLFSAGPIIALIPAIAAFAVIPFASTLHWDYLNQVLPRTGEISGPAVPMQVASIN
                     VGILFIFAIASTGIIGAAISGYASDNKYSLLGGIRAASQMVSYEVVLGLTLVPAFMVY
                     ASLRLEEMATWQYEHLWGVFIPPLTLASINFFVAAVAETKRVPFDLPEGESELVGGYL
                     TEYSGMKFGMFFMGEFVEVVSLAALASVIFLGGWDVPFLFRDGFEFSLPFVAGSVSVP
                     LVHWLVVAIQFVFFLFKIIVLIWFQLMIRWSLPRFRYDQLMDLCWKGLLPVSLLNILV
                     VGAFILSGWLDGWVAFFEQLL"
     gene            8150..8743
                     /locus_tag="VFG22_08070"
     CDS             8150..8743
                     /locus_tag="VFG22_08070"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HET6416240.1"
                     /translation="MTVEVKVIKKPGRTVAEQVYLPEIAKGLGFAMGRFFKNTFGPQD
                     KKEIATIKYPEETVEYPERFRGIHRLTQRTDGSPRCVACLCCSTACPAQCIHIEPAEY
                     EPEDPRHGYERYPAVFVIDELRCIFCGYCVEACPCDAIRMDTGMHMPPSTNRDHFLFD
                     KETLLSIPGRDGTYLTANPRHEPGDPTHPGIDRERGH"
     gene            8746..9594
                     /locus_tag="VFG22_08075"
     CDS             8746..9594
                     /locus_tag="VFG22_08075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6416241.1"
                     /translation="MSEERAASRTAIMVAAYRGRASAMRNAICNDPWALDLAGPEGEA
                     LSRRWDAHSPSMELWMALRTRYIDDSVTRAIDRGIGQVVILGAGLDTRAARLAKPGVR
                     FFELDQPASQLHKRTQLSRFDGYPVDAATFVPCDFERDDFVELLESAGLRRDRPSCFV
                     WEGVIYYLIEAAARSTLERLASEFDPRSLLIFDYLNSKMARSSPRLRDEDRAMKEIID
                     ELGEPMRFGIDDPTPLMAESGYRLLRTVSFDELALEYTGTYSRDRFFRFQSIALASPS
                     AELTPW"
     gene            9588..10355
                     /locus_tag="VFG22_08080"
     CDS             9588..10355
                     /locus_tag="VFG22_08080"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6416242.1"
                     /translation="MVERRRLGARTVTFRHEPGRTPSLICVHGAADNHQVFDRLLDGM
                     PEVDRYAINLPGRAGTDGPPLQNVREMEAFLSSLLEAELDDGDYIVVGHSLGGAVAIE
                     HALRCTSARLKGIVLLATGARLRVHPMIPQLFEQASKSGQMPPLTPGLYERRTDPELV
                     AEASKKRLATPIETGSQDWRAADSFDRMHDLGNIRVPALIVAGTDDALTPSKYAEYLG
                     AHIPQNVMKLFDGACHMFVLERASELGTAIERFAAAR"
     gene            10443..12347
                     /locus_tag="VFG22_08085"
     CDS             10443..12347
                     /locus_tag="VFG22_08085"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HET6416243.1"
                     /translation="MIDIDSALQDRFGLRAFHSWQREAIEELLHGRKNALVIAPTGGG
                     KSLCYQLPATVLEGTTIVISPLIALMEDQVRALTERGITATYLASTLEPEELGQRERE
                     IFNGRYQLVYVAPERLESPWLVDRLASLRPPLLAIDEAHCISQWGHDFRPSYLRLGDV
                     IKRLQPSRVLACTATATPIVRREIQERLNLGSERSAVILRGFSRPNLHLAVVESDNSN
                     ERRNLMLSALRAALGSPDDLKGGAIVYAATRRNTEKIAEAIAKAGWRVAAYHAGLPAS
                     VRERVNRRFARAELDVVVATNAFGMGIDRPDIRTVVHAQAPGSVEAYYQEVGRAGRDG
                     QPAQGLLLTSSGDLGLRRRLIGFSGGRDPGSVVDPMHVQQQWSLFLDLMRYVEAGSCR
                     HDFILRYFGDEQETLGGCGHCDVCERLDEQGEDGDERATSTDEALIVRKALSGVARNQ
                     GRAGLTAVSEMLHGTDNERLRRLGLTELTTHGLLEKHPKAWVLALLRRLITAGLVDLS
                     NSEFPIPYLTPLGVATMKGEEPVRVLVPPPDAGRARAAKKERRRADHTLPEHVDSSLF
                     ESLREKRLDLARAKGVPAYVICHDRTLAEIAAYKPTSLEALADIHGMGPARIERYGAP
                     FLETVSSHAS"
     gene            complement(12363..>13034)
                     /locus_tag="VFG22_08090"
     CDS             complement(12363..>13034)
                     /locus_tag="VFG22_08090"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HET6416244.1"
                     /translation="ERIERETLGVERDVAGEDQKLEGVVRLTATEMLTTRFIAPHLRR
                     FRERYPEIQLELQCTNLDVNLARREADIALRLARPTQDDLIIKRLSFIHLGLYASLEY
                     VDRFGLPKDSLAGHQMILFANVRPFRRENDWIEARMDGARVALRSDSVSATYSATVAG
                     TGIALLPCLVADHDPHLVRVPLEGAPAPRQIWQAVHKDLRDSARIRAVLDFLGKILAQ
                     PPEAT"
BASE COUNT         2480 a         4125 c         4021 g         2408 t
ORIGIN      
        1 tttcgcgggc ggacgaagag caccactcga tggtccgcgt gttggttcgt gagcaggagc
       61 aggctctccg gctcatcgaa gccgctcaag tagtgcaggt cgctgtcctg gcgatatggg
      121 tgttcgacgt cgtgatttcg aagcgcgagc ggggccgagg gaaatacggc aacgccgtcc
      181 cccatggcat cgagaagttg ctggcggcgc gctgcgaact ccgacatctc acgatcctag
      241 cagctagctt ctcggctgca tctcgccgct cttgtagcga gaccagtagc tcttgagagc
      301 atcgctcact ttgccgctca acagaaaggc cccgaggatg ttgggaaacg acatgcccaa
      361 gatcatcagg tccgagaaat cgatcacgtt gccgagcttc aagaccgacc caagcacgat
      421 gaacccgagg aacagcacct tgtagatcat cgagaactgc gcaccaaaga ggaaggtcca
      481 gcaacgctcg ccgtagtagc tccaagagat catcgtcgag aaggcgaaca agaacacggc
      541 gaggctgagc acgttgggaa accagggaag gaccgtcgcg aacgccttcg acgtcatgag
      601 aacgccttcc tcgccaccac cttcgtaggc tccggtgatc accaccacca agccggtcat
      661 cgtgcagacc acgatcgtgt cgatgaacgg ctcgagcagg gctacgatgc cttccctcac
      721 cggctcgtcg gttgccgcag cggagtgcgc gatcgatgcc gaacccaccc ccgcttcgtt
      781 cgaaaatgca gcgcgccgga agcctgtgac gaggacgccc agcattccgc caaatccggc
      841 ttcgggtgaa aacgcttcgc cgacgatctt ctgcaaggag gggaggatct ggtcgaagtt
      901 cgtgatgatg atgaacaggc ctgcaacgag ataaatgccg cacatcaccg gaacgatgaa
      961 tcccgctact tggccgattc tgcggatgcc gccgatgatg acgatgccga cgaggaagga
     1021 gagggtgacc ccgtaggcga gtgaacccca acctccggca aagaacggca tgatgtcggc
     1081 aacctgcgca tagctctggt tggcctggaa catgttgccg ccgccaaagc ttccaccgat
     1141 gcacatcacc gcaaaaacga cagcgaggac acgaccgagc cgcggaagcc cgagctcacc
     1201 gaggccgcga tcgagatatc gcatcggccc gccgaggatg tgcccatcgt ccgcgacttc
     1261 gcgatacatc tgccccaggg tgcattcggt gaacttcgac gacatgccga ggaagcccgc
     1321 tacgatcatc cagaagatcg cgccgggtcc tcccaccgcg accgcgaccg ccacaccggc
     1381 gatgtttccg agcccgaccg tggccgagag cgcggacgac agcgcttgaa agtgcgtcac
     1441 ctcgccgggg tcgtccggat catcgtagtg accggtagtg accacccagg cgtgcctgaa
     1501 ggcacgcaag ttgatgaagc cgaatcgcag cgtgaaaaac gtcgccccgg cgatgagcca
     1561 gaagacgatg aaggggagct tcaggtcatg gtctgatccg ttgtcccaga agatcagatc
     1621 gaagaagagg accttggcga ttggggcaac cacgtgcgca ccaaaaccgc catcgagcga
     1681 ctgcagcacg cctccgatcc ctgtctccgc gctggaacgg gcctcagccc gggcgacggc
     1741 gggatacaaa ccggcccata aggcggcaac tgctatcaga tgtctgccca ttcccctagg
     1801 caaccatcgc acaacactga aattctctcc agttttattc tgagtagaat attcgctctt
     1861 aaacgcattt ctcctgaaca accaagggca accgtgacga tgatgcgcgg ctgcacggat
     1921 ccgtcgaaga cgttctcgaa gagcgcaccg tgtctggagg cagcgtgcag tacgggcccg
     1981 tcgttcgcgc agaagagctc gggaccgcat ccgctcggga aggatccggc tgacactgcg
     2041 cggcgagcag atcgcctggg acggaatcag cgatcttggc gctcagccac gaatcgatga
     2101 ccacactgcc tccggtaccg gctctgcgtt cgcggcgtcg aggaccgccg gcggcccctt
     2161 gggattgagc tcactcatct tcggagcttt gctgctgaat cgccgccgcc gaagataggg
     2221 gcgagcggcc ggcgaccttc attgacaccc cctgagggtg atactaagca cccctcgacc
     2281 ggcgcaacca gccccgtcgc ggagtccgac tctctatgtt ctcggcatac ttaccggtgt
     2341 tcctgatgct tggcgtcgcc gtgctcgtcg cggtcggcgt cttcgccctc acctcggtgc
     2401 tcggcccgaa gaacatgacg gccgagaagg cgatccccta cgaatctggc tccgagacca
     2461 cgggggcaag ccacatccgg ctgagcatca agttttatct cacggcgatc ctgttcgtcg
     2521 tcttcgacat cgaggcggtg ttcctctacc cctgggccag tctctttcga gatctcggat
     2581 gggtcggctt catcgagatg ttctcgttcc tcgtcgttct gggggtgacc ttggtctacg
     2641 cctggagaaa gggtgcgctc aattgggaga gttgaaggtc atcgatggtg gcggcgaggt
     2701 tgcggtcacg acgcgcctaa gcgacctcct ccagtgggcg cggaagtggt ccctctttca
     2761 gtatccgttc gtgacggcct gctgcggcat ggagttcatg gctaccgctg cgccgcgcta
     2821 cgacatcgca cgcttcggcg cagaggcccc tcgtttcagc ccgaggcaat ccgatgtgct
     2881 ctgggtcgtg ggaacgatca gccagcggca agggccggcg gttcgacgca tttgggaaca
     2941 gatggccgat ccgaaatggg tcatcgcgtt cggcacatgt gcgtcctgtg gcgggttcta
     3001 cgacaactac acgacgattc ccggcgtcga taaggtcatt ccggtggacg tatacatccc
     3061 cggctgccct ccgcgccctg aagcggttct cgatgcgctg atgcttcttc aagagaagat
     3121 tcaagccaac gaccgcaagc ccatcgtcgt gcctccgagg ttggtgcccg aacgcgcacg
     3181 aggccggtcc ggggagggcc agtcatgagc caagcggtcc tcgaccggct cagcacccaa
     3241 ttcggagaac gagtcctcgg aacgagcgat gcgttcggcg actacgagtt gcagatcgcc
     3301 ctcaaagact gggtcgaagt cgcgcaattc atgaaagccg atgagcagct tcggatggat
     3361 cacttcatcg acctcacggc gatcgactat ccggagcgcg agtcggcgcg gtttgacctc
     3421 acgctgacga cgcgctcgtc gtcgaactgt gaccggattc gcattcgcac atacgtcaag
     3481 gaagacgaag agcccgggac cctcagtcaa gtctgggccg gggcgaactg ggcggagcga
     3541 gaggtctggg acatgttcgg gatcaggttc aagggccacc ccgacatgag gcgcatcctt
     3601 ctctacgagg agttcgtcgg gcatccgctc cgcaaagact atccgatcga caaggcgcaa
     3661 ccgctggttc cgtaccgcga ggtagacgat atcgagaagc tccccccgtt cggcatcgag
     3721 gagggtcagc ccttcgcgcg gatcgattgg gagtcgcggc tggccggcgg ggaccagcag
     3781 gtgtcgcctt cgatcggcgt acagcagggc cagcgccgga cgctgagcga ttccgaggtg
     3841 gccgagcacc tgcacgcacg catcgcggcg cgcatggcca aggaagcaga cacggcgtcg
     3901 agcgatgccg gcgagaaagg ttcgggggag tagatggaac ccgctgacga gctctcgaac
     3961 gacgcaactg cgctcgagct ccactccgac ccgatgcgct tgaacatggg accgtcccat
     4021 ccggccatgc acggtacgat ccgcatggtc gtcgacctcg acggcgagaa tctggtcaac
     4081 ctcgacgtgc agcccggcta cctgcatcgc ggcttcgaga agtcctgcga gcgcggaacc
     4141 tgggcacagg tctttccata cgtcgaccgg ctcaattacg tgtccccgat gctcaacaac
     4201 gtgggctggt cgctggcggt cgagaagctc cttcaaatcg aggtgcccga gcgctgccaa
     4261 tactacaggg tcattctcgg tgagctggcg cgtatcgcag accacttcac ttgtaacggc
     4321 gcctcggcga tggagcttgg cgcgttcacc cccttcctct ggctgctgaa ggtgcgcgac
     4381 tgggtctggg acatcctcga gcgcgaaacc ggggcgcgca tgacccacag cttcggccgg
     4441 atcggcggga tggcagctcc gcccacgatc aacttcaagg acgacgtgct ccgcctgatc
     4501 ccagaagttc acaaggtcgt gaaagaaacc gagatcatgc ttctccgcaa tcggatcttc
     4561 ctcgaccgaa tgcagggagt gggagtgctc accaaggagc aggcgctctc gtacggcgtc
     4621 acgggtccgg tcgcgcgttc gaccggcatc ccctacgacg tccgcaaaga tcacccctac
     4681 ctggtctacg accgattcga cttcgacgtg cccgtcggcg aagacggcga caactgggat
     4741 cgcttcatgg tgcgtctcga agagatccgc caatcggtgc gaatcatcga gcaggccatc
     4801 gaacagatgc ccgacgaagg cccggtcaac gtagacgatc cgcggatcgt ctatcccgca
     4861 aaaggcgacg tttacacgac gatcgaagcg accatcgctc atttcaagct cgtgatggag
     4921 ggcctgcgca cgcccaaggg cgaggtctac agctttaccg aaggcggcaa cggcgagctc
     4981 ggcttccaca tcgtctccga tggaagcggc acgccctacc gagtgcgcgt ccgtccgccg
     5041 tgctggtaca acctcgcggc agctcgcgaa atgctgctcg gtggcatgat cgcggacatc
     5101 attccgactt tcggatcggt caacatgatc ggcggggagt gtgatcggtg agcgatgcct
     5161 aagttcgttt tagatggcaa agagatcgcg ttcgagccag gggagacggt catgcaggcc
     5221 gcttggcgcg aggggatcga gattccgcac tactgctggc atcccgggct ttcgatcgcc
     5281 gccaactgcc gcatgtgcct cgttcaaatc gagagcgggc gccaaatggc catgccccag
     5341 ctcgccttcg acgaagccaa gggcgaatac gtccccgcca cgaagccgaa gttgcagccc
     5401 gcttgtcagc tcgccgctgc cgaagacatg gtcatctcgt cgacgaacga cgaggtcaag
     5461 aacgcgcaag cctcggttca ggagtttctg ctcctcaacc acccagtcga ttgcccgatc
     5521 tgcgatcagg ccggcgagtg caagcttcag gattactact acgaacatca ggggacgctg
     5581 aagcgcaagc tcaccgagcc ggtgcacaag cccaagggtg tgcgattcgg accgacgatc
     5641 gtctacgatg cggagcgctg catcatgtgt acgcgctgca ttcgcgtctg tgacgaggtc
     5701 gcgaaagacc acgttctcga catgcgtgag cgtggaaaca agaacgagat catcctcgct
     5761 ccgggtcgcg agctcgacca caagtacacc ctgatgaccg agcacgtttg tcccgtgggt
     5821 gcgctcacga gccaagattt ccgattcaag gcgcgagtct ggttcctgaa gtcacggccc
     5881 agcgtctgca ccggctgcgc gacgggctgc aacagccacg ccgacttcga tccgcgcgat
     5941 ggcaaaatct accgcctccg tccacgcgac aacgaggccg tcaacaagtt ctggatgtgc
     6001 gacgacggca tgatgagcta ccaccgtcac gacacggacc gggtcttgat gccgaccgtc
     6061 ggccggggcg acgaacgaac cccgataggc gaacacgagg cgctcgagct tgccgccgag
     6121 cagctgcgag gggtggatcc gtcgaagcta gccgtggtct tgagcgcaca gagcagcacc
     6181 gaggataacc tggcgctcgc caagctcgcg gccacgctcg gtgccgggca gctctatctt
     6241 ggagcgctcg gtggctggga aggcgacgac attctgcgaa gcgcggacaa caacccgaat
     6301 cgtgccggcg ccacgcaggc tgcatcgggc gctcctttga aatcggtcgc cgagctcgtg
     6361 gccgatgtgc aaggcggcac cgttcagggt gtgctcgcgc tcggctgggc gaccgccgag
     6421 aagctcgagg agctggcgcc cctcgtcgcc ttgaacgccg tgatttccct cacgagtcac
     6481 gtgggtcctc ttccagcggt agcctcgatc acgattccag ttgctgacac gttcgagatc
     6541 gacggcagct tcgtcaacga gaagggcatc gcccagtcct tcggaccagt gctcccgctt
     6601 cccgcaggag tcgaaccggc gtggaagacc gtttcggacc tggcgcgcgt cttgggcgcg
     6661 aagctcggct tcgacgattt gaaggggcta cgacagggtc tccaagacgc ggcggaggcg
     6721 gcccaatgag cacgacgttt ctcgtagtct cgtcgatggt gaaggtgctc gtgattctgc
     6781 tgatcacggt cggggccttt gcgccgattc tcatttgggc agagcgccgg cagagcgcga
     6841 tgattcaaga ccggctcggg ccgagtaggg cggccatcgt gctcccggcc tcggcgcttg
     6901 cgttcatcga ccgggcgcga cttccaattt ggcgcctggc agcgctgacg ggcatcgtgg
     6961 ccatcgtcgc attggtcaca ctcacgttcg tcgccatcaa gggcagcaat ccacttcccg
     7021 tttctacgtc cacgctcacg acgagcctcg tcggcttcgg tgtggcgtcg gtcctgcttc
     7081 gtgccggcta cggggtgcac agcatgatcg ttcgcaatgg cggcaagatc atgctcatcg
     7141 gcctgcttca ccccttggca gacgcgatca agttcatctt caaagaggac ttcgttcctc
     7201 ccaaggccga caagctcttg ttctcggccg gcccgatcat cgcgttgatc ccggcgatcg
     7261 cggcatttgc ggtgatccct ttcgccagca ccctgcactg ggattatctc aatcaggtcc
     7321 ttccgcgcac cggcgagatc tccggacccg cggttccgat gcaggtcgcg agcatcaacg
     7381 tcggaattct cttcatcttt gccattgcca gcaccggcat catcggtgcg gcgatctccg
     7441 ggtacgcatc cgacaacaag tacagtctcc tcggcggcat ccgtgctgcg agccagatgg
     7501 tgagctacga ggtcgtcctc ggcctcaccc tcgtaccggc cttcatggtg tacgcctcgc
     7561 ttcggctcga agagatggcc acctggcagt acgagcatct atggggtgtc ttcatccctc
     7621 ctctgacctt ggcctcgatc aatttcttcg tggccgcggt cgccgaaacc aagcgtgtcc
     7681 cgttcgacct ccccgagggc gaaagcgagc tcgtcggtgg ctacctcacg gagtactcgg
     7741 gcatgaagtt cggcatgttc ttcatgggcg agttcgtcga ggtggtgtcg ctcgcggcgc
     7801 tggcgtctgt catctttctc ggcggatggg atgtaccatt cctcttccgc gacgggttcg
     7861 agttctccct gcctttcgtg gcgggtagcg tttcggtacc cctggttcac tggctggtcg
     7921 tggccattca attcgtgttc ttcttgttca agatcatcgt cttgatctgg ttccagttga
     7981 tgattcgctg gtcgcttccg cggtttcgct acgatcagct catggatctc tgctggaaag
     8041 gtctgctccc cgtctccttg ctcaacatcc tggtcgtagg tgcgttcatt ctgtcgggct
     8101 ggctcgacgg atgggttgca ttcttcgagc agctcctctg aggtcccgca tgacagtcga
     8161 agtcaaagtc atcaagaagc ccgggcgcac cgtcgcagag caggtctacc tccctgagat
     8221 cgccaagggc ctcggcttcg caatgggccg attcttcaag aataccttcg ggcctcagga
     8281 caagaaggaa atcgctacca tcaagtatcc ggaggagacg gtggagtacc cggaacggtt
     8341 ccgtggcatc catcgactca cgcagcgcac cgacggttcg ccgcgctgcg tggcctgcct
     8401 ctgttgcagc acagcgtgtc ccgcacagtg catccacatc gaacccgctg agtatgaacc
     8461 cgaggatccg cgtcacggct acgagcgcta cccggcggtg ttcgtgatcg acgagcttcg
     8521 ctgcatcttc tgtggctact gcgtcgaggc ctgcccttgc gacgcaatcc gcatggacac
     8581 ggggatgcac atgccaccgt ccacgaaccg cgatcacttc ctcttcgaca aagagacgct
     8641 gctttcgatt ccgggacgtg acggcacgta tctcaccgca aatccgcgtc acgaaccggg
     8701 cgacccgacg catccgggga tcgatcgcga acgcgggcac tgagcgtgtc cgaggagcga
     8761 gcggccagcc gtacggccat catggtcgcc gcgtatcggg gtcgggcgtc cgcgatgcgc
     8821 aatgccatat gcaatgatcc ctgggctctc gatctcgccg gcccagaggg tgaggctcta
     8881 tcgcgccgtt gggacgcgca ctctccttcg atggagctct ggatggcgct acgaacgcgg
     8941 tacatcgacg actcggtgac gcgggcgatc gaccggggga ttgggcaggt tgtgatcctc
     9001 ggcgcggggc tcgatacccg ggcagctcgg ctcgcaaagc ctggcgtacg ctttttcgag
     9061 ctcgaccagc ccgcgagtca gctccacaaa cgtacacagc tttcgaggtt tgacggctat
     9121 cctgtcgatg ctgcgacctt cgtcccatgc gacttcgagc gtgatgactt cgtggagctt
     9181 ctggaatccg ctggcctccg gcgcgaccgg cctagctgct tcgtctggga aggcgtcatc
     9241 tactacttga tcgaagcggc ggcacgatcg acgctcgagc ggctcgcctc ggagttcgat
     9301 ccccgctcgc tcctgatctt cgactacctg aacagcaaga tggctaggtc gagcccgcgg
     9361 ctccgggacg aggaccgcgc catgaaggag atcatcgacg agctcggtga gccgatgcga
     9421 ttcggcatcg acgacccgac ccccttgatg gccgagtcag gataccgcct cctgcgcacg
     9481 gtttccttcg atgagctagc gctcgagtac accggaacgt actcacgaga tcgctttttt
     9541 cgcttccagt cgatcgcgct cgcgagtccg agcgccgagc tcacgccgtg gtagagcgcc
     9601 ggcggctcgg tgcacggacc gtcacctttc gacatgagcc cggccggacg ccctcgttga
     9661 tctgcgtcca cggtgccgca gacaatcacc aggtcttcga ccgcttgctc gatggcatgc
     9721 cggaggtgga ccgctacgcg atcaatctcc cgggacgcgc cggaaccgat ggacctccgc
     9781 tccaaaacgt ccgcgagatg gaggcgttct tgtcgagcct cttggaggcc gagctggacg
     9841 acggagacta catcgtggtt ggtcattccc tcggcggcgc cgtggcgatc gagcacgctc
     9901 ttcgctgcac ttcggctcgc ctcaaaggaa tcgtcctgct ggccactggg gcgcggctcc
     9961 gcgttcatcc catgatccct caactcttcg agcaagcatc gaagtccggt cagatgccgc
    10021 cgctcacccc ggggctctac gagcggcgaa cggaccctga gctggtcgca gaagcttcga
    10081 aaaagcgcct cgccacgccg atcgagacag gcagtcagga ctggcgcgcc gcggacagct
    10141 tcgaccgcat gcacgacctc gggaacatcc gggttccggc tctcatcgtc gcgggtaccg
    10201 atgacgccct gacgccttcc aagtacgccg agtacctggg ggcccatatt ccgcaaaacg
    10261 tgatgaagct gttcgacggc gcctgtcaca tgttcgtgct ggaacgggcg tccgagctcg
    10321 gcaccgcgat tgagcgcttt gccgcagctc gttaggcaac gcacccgggc tcgcaacgcc
    10381 tgcgatgcgc ggccgatagc cctggcatgc agtcatcggc cgcactaggt tgccctccgt
    10441 tcatgatcga catcgactcg gccttgcagg atcgctttgg tcttcgtgca tttcattcct
    10501 ggcaacgcga ggcgatcgag gagctcctcc acgggcgaaa gaacgccctg gtcatcgcgc
    10561 caacgggcgg gggcaagtcc ctctgctacc agcttccggc cacggtcctc gaaggcacga
    10621 cgatcgtcat ctcgccgctg atcgctttga tggaagacca ggtgcgcgca ctgaccgagc
    10681 gggggatcac cgcgacctac ttggcatcga ccctcgaacc ggaggagctc gggcagcgcg
    10741 agcgcgaaat tttcaatggc cgttaccagc tcgtgtacgt cgcgcccgag cggctcgaat
    10801 cgccctggct ggtcgacagg ctcgcgagcc tccggccgcc gctcctggcg atcgacgaag
    10861 cgcactgcat ttcgcaatgg ggtcacgact tccgcccgtc gtatctgcgt ctcggcgacg
    10921 tcatcaagcg cttgcaacct tcacgggtcc ttgcctgcac ggcaaccgca actccgatcg
    10981 tgcgtcgcga gatccaagaa cgattgaatc taggctcgga acgaagcgcg gtcatcttgc
    11041 gaggcttctc gcgtcccaac cttcatctgg ccgtcgtgga aagcgacaac tcgaacgagc
    11101 ggcgcaactt gatgctctcc gcgcttcgag cggcgctcgg cagccccgac gatctaaagg
    11161 gcggagcgat cgtatacgca gccacgcgca ggaataccga aaagatcgcg gaagccattg
    11221 ccaaagccgg ttggcgagtc gcggcctacc acgcgggcct ccccgcttcg gtgcgtgaac
    11281 gcgtcaaccg acggtttgcg agagcggagc tggacgtggt cgttgcgacc aacgcgttcg
    11341 gcatgggcat cgaccggccc gacattcgaa ccgtcgtcca cgcgcaggcg cccggttcgg
    11401 tagaagcata ctatcaagag gtcggccgcg ccgggcggga tgggcagcca gcgcagggtc
    11461 tcctgctgac cagctcgggg gacctcggtc ttcgacgtcg attgataggg ttcagcgggg
    11521 gacgtgatcc gggtagcgtg gtcgatccca tgcacgtgca acagcagtgg agcctgtttc
    11581 tagacctgat gcgctacgtc gaagctggga gctgccgtca cgacttcatc ctgcgctact
    11641 ttggcgatga gcaggagacg ctcggaggct gcggtcactg cgacgtctgc gagcgactcg
    11701 acgagcaagg cgaagatggc gatgaacgag ctacttcgac cgacgaagca ctcatcgttc
    11761 gcaaggccct ctcgggcgtg gcgcggaatc agggccgcgc ggggcttacc gcggtgagcg
    11821 agatgcttca tgggacggac aacgaacggc ttcgtcggct cggcctcacg gagctcacga
    11881 cacacggcct tctcgagaag cacccgaagg cctgggtgct cgctcttctg aggcgcctga
    11941 tcaccgcggg cctcgtcgac ctcagcaaca gcgagttccc cattccctat ctcacgcctt
    12001 tgggcgtggc cacgatgaaa ggggaggaac ccgttcgggt cctcgttcca ccgccagacg
    12061 cgggccgtgc ccgagcggcc aagaaagagc gccgtcgcgc cgatcacacg cttcccgagc
    12121 acgtcgactc atcgctgttt gaaagcttgc gcgagaagcg gctcgacttg gcgcgcgcaa
    12181 aaggcgtgcc cgcatacgtg atttgccacg accggacgct tgctgaaatc gctgcgtaca
    12241 aaccaacctc actcgaggcg ctcgcagata tccacggcat ggggccagcg cgcatcgagc
    12301 gctacggagc gccgttcctg gaaaccgtca gctctcacgc gagctgagcg atggtgcagg
    12361 gcctaggtcg cttcgggcgg ctgtgccaga atcttgccga ggaagtcgag aacggcacga
    12421 attcgtgcgc tgtcgcggag gtctttgtgc acggcctgcc agatctgccg aggtgcgggg
    12481 gcgccctcga gcggaacccg aaccaggtgt gggtcatggt cggccaccag gcaaggaagc
    12541 agagcaatgc cggtgcccgc aacggtagcc gagtacgtcg cggacacgct gtccgaacga
    12601 agagcaaccc gggcaccgtc catccgagct tcaatccagt cgttctcgcg tcggaacggg
    12661 cgaacgttgg cgaagaggat catctggtgc cctgcgagcg aatccttcgg gagcccgaaa
    12721 cgatcgacgt actcgagcga cgcgtacaag ccgaggtgaa tgaaagaaag acgcttgatg
    12781 atcaaatcgt cctgtgtggg tcgagcgagt cgaagtgcga tgtccgcctc ccgcctggcg
    12841 aggttgacgt ccaagttcgt gcactgaagc tcgagctgga tttccggata gcgctcgcga
    12901 aaacgtcgaa ggtgcggcgc aatgaaccgc gtggtgagca tctccgtcgc ggtgagccga
    12961 accacgccct cgagtttctg atcttctccc gcgacatccc gctccacgcc gagcgtctct
    13021 cgttcgatcc gctc
//
LOCUS       DASRZF010000181         2288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1497921, whole genome shotgun sequence.
ACCESSION   DASRZF010000181 DASRZF010000000
VERSION     DASRZF010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1497921"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..1049)
                     /locus_tag="VFG22_08095"
     CDS             complement(6..1049)
                     /locus_tag="VFG22_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416245.1"
                     /translation="MTEPAVPLIVTAAWDAYGDHREVVKVREISAPVSTNSVYLLVLD
                     DGHAVVAKRSSYGSYVHFRQDHYRIHEWIHRLRGTRYAAFLAPVLLKNGEVFTYREED
                     EWVVFYSQQPFYDFLPKVLSDDQVESFGQEMGLFHRACLEISDRVKPTWQSLGADVAS
                     LHDAIGSAEWRAERGFMDSSISFVRAHCDAFLGNADDLGYHEWTKIPILIDWNLGNFS
                     VGMDGDGFKFFSRWDYDWFRIEPRMLDLYFCARVCRAEGDQTAFSYTADPLFDERFMK
                     FLRAYHEVHRLTKEELLFLKESYRFFILNYVLRVGEHFFRPEICTRLQHEAVEEYLPQ
                     LELLDFGPLLEIL"
     gene            complement(1067..1981)
                     /locus_tag="VFG22_08100"
     CDS             complement(1067..1981)
                     /locus_tag="VFG22_08100"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HET6416246.1"
                     /translation="MSDRVEKILANYTGENPGVIGNLRRLMNTGRLAGTGKLVILPVD
                     QGFEHGPARSFAPNPPGYDPAYHPALAVEAGCNGYAAPLGFIESIAHQYAGQLPLILK
                     VNNSDSLGGPDEPCSALTSSVDDALRLGCAAIGYTIYPGSGLRNPMYQDLRDLINDAR
                     AVGLPTIVWSYPRGAGLSSEGETAIDVVAYAAQIACQLGAHVVKVKPPTDLVFQKAAK
                     KSYENVPIGTLAERIQHVVQAAFNGKRIVIFSGGAAKGTDAVLEEISQIAKGGGYGSI
                     VGRNAFQRSNAEAVKLLHDIMDLYAAQA"
BASE COUNT          424 a          701 c          726 g          437 t
ORIGIN      
        1 cgctgctaga gaatctccag cagtggccca aaatccagca gctcgagctg cggcagatac
       61 tcctccaccg cctcgtgctg cagccgggtg cagatctccg ggcggaagaa gtgctcacct
      121 acccgaagca cgtagttcaa aatgaagaag cggtaggatt ccttgaggaa taggagctct
      181 tccttcgtga ggcggtggac ctcgtggtac gcgcggagga acttcatgaa gcgctcgtcg
      241 aagagggggt cggcggtgta gctgaaggcg gtttgatcgc cttcggcccg acagacgcgc
      301 gcacagaagt agaggtcgag catccgcggc tcgattcgga accagtcgta gtcccaacgc
      361 gaaaagaact tgaagccgtc cccgtccatg ccgaccgaga agttcccgag gttccagtcg
      421 atcaagatgg ggatcttcgt ccactcgtgg tagccgagat cgtccgcatt gccgaggaaa
      481 gcgtcgcagt gcgcgcgcac gaagctgatg gatgaatcca tgaagccgcg ctccgcgcgc
      541 cactcggcgc taccgatggc atcatgaagg ctcgccacat cggcgccgag cgactgccag
      601 gttggcttta cccggtccga aatctcgagg cacgcccggt gaaagaggcc catctcctga
      661 ccgaacgatt ccacctggtc gtcgctgaga accttcggca ggaagtcgta gaacggctgt
      721 tgcgagtaga aaacgaccca ttcgtcctcc tcgcgatagg tgaagacctc gccgttcttg
      781 agcagcaccg gcgccaggaa cgccgcgtag cgggttccgc gaagccgatg gatccactcg
      841 tggatgcggt agtgatcctg gcggaagtgc acgtaggagc catagctcga gcgctttgcg
      901 accacggcgt gtccgtcgtc gaggacgagc aggtacaccg aattcgtcga caccggggcg
      961 ctgatctcgc gcaccttcac gacttcccgg tgatccccgt aggcatccca ggctgcggtc
     1021 acgatgagtg ggacggcagg ctcagtcacg cgtcgatgaa cgcttcttac gcttgtgcgg
     1081 cgtaaaggtc catgatgtcg tgcagaagct tcacggcttc cgcgttggag cgctggaacg
     1141 cgttccggcc gacgatcgaa ccgtagccgc cgcccttggc gatctggctg atctcttcga
     1201 gaactgcatc ggtacccttt gctgcgccgc cagagaagat gacgatgcgc ttcccgttga
     1261 aagcggcctg cacgacgtgc tgaatgcgct cggccaaggt gccgatggga acgttctcgt
     1321 agctcttctt tgccgccttc tgaaaaacca agtcggtcgg gggctttacc ttcactacgt
     1381 gcgcgccgag ctggcaagcg atctgtgccg cgtaggccac gacgtcgatc gcggtctcgc
     1441 cctctgagct cagaccggca ccgcgcgggt acgaccagac gatcgtcgga aggccgaccg
     1501 cccgtgcgtc gtttatcagg tcgcgcaggt cctggtacat cggattgcga agcccgctgc
     1561 ccgggtagat cgtgtagccg atcgcagcgc agccgaggcg cagagcgtca tcgaccgacg
     1621 aggtgagcgc ggagcaaggc tcgtccggtc cgccgagcga gtcgctgttg ttcaccttca
     1681 agatcagagg aagctgaccc gcgtattggt gcgcgatcga ctcgatgaag ccgagcggcg
     1741 cggcgtagcc gttgcaaccg gcctcgacgg ccagcgccgg gtggtatgcc gggtcgtagc
     1801 ccggaggatt tggcgcaaac gatcgcgccg gtccgtgctc gaaaccttgg tcgaccggca
     1861 ggatcacgag cttgcccgtg ccggcaagcc ggccggtatt catcagccgc ctgagatttc
     1921 caatgactcc agggttctcc cccgtgtagt tcgcaaggat tttctcgact ctatcactca
     1981 tgcccgcagc ctccgttctt cgcccatgac cctagctcag agatcgagga cggtcatgcc
     2041 gtttgcggaa ggaatttcgc gcaagtcttg ccccgaacgc cgggcgcccg gggggagggg
     2101 tccgggaacg gggtctcctg ccctccgtca ggccctgggc tgctgggccg ccggggcgct
     2161 ctctgcgtag aacgctgcaa tctccttcgc gtacttctca ttgacgatgt tgcgcttcac
     2221 tttgagggtg ggcgtgagct cacctccatc gacgctgaac gtggtcggga gaatcttgat
     2281 cttcttga
//
LOCUS       DASRZF010000182         4205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_466293, whole genome shotgun sequence.
ACCESSION   DASRZF010000182 DASRZF010000000
VERSION     DASRZF010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466293"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..2326
                     /gene="clpA"
                     /locus_tag="VFG22_08105"
     CDS             32..2326
                     /gene="clpA"
                     /locus_tag="VFG22_08105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HET6416247.1"
                     /translation="MAGPVIDKELQATLRKAFDRAGLMRHEYVTLEHLLLALTEDPKA
                     SKALHACGADIRRLRKKLEEFIQERLSRVPESVSVDPRQTLAVERVLQRAAIHAISSE
                     MKTIDGSNVLVQLLKEEESFAAFILQEEGVNALDLKRYIAHGVGTDIVPGESDAYTDT
                     DFEDDEGEGAAGRDPLEAFATNLITEAAEGRIDPLIGRNEELDRTIQVLCRRRKNNPV
                     FVGEPGVGKTAIAEGLALRIHEGKVPEILEDAEIYSLDMGALLAGTKFRGQFEERLKA
                     VMKRLQELDNAILFIDEIHTIVGAGATTGSSMDASNILKPALASGKLRCLGSTTYADF
                     KHLERDRALARRFQKIEVLEPTVADTIEILKGLRQYYEDHHDVKYDDDALAAAAQLAQ
                     KHIVERFLPDKAIDVIDEAGSLERMRPEGERTHRITVKEIERVVSKMARVPVESVSAE
                     ERDQLKELGPELRKVIFGQDEAIDKVVSSITLARAGLRADNKPIGSFLFAGPTGVGKT
                     ELARQLARVMGVELLRFDMSEYGERHSVSRLIGAPPGYVGFDQGGLLTDAVRKHPHAV
                     VILDEIEKAHGELFNILLQVMDHAKLTDNNGREADFRNIVLVMTTNAGAFEATEKVVG
                     FGGGDGPSDFAKGRAKQAMERIFTPEFRNRLDAVVYFAGLRKEVIRKVVDKEIGLLSA
                     MLSEKQVRLELDDAARDWLADHGYDPQMGARPMARLVEQKLKKPLAEAMVFGDLKEGG
                     TARAAVQDGEIVLSFDETGPPTVH"
     gene            2393..3118
                     /gene="aat"
                     /locus_tag="VFG22_08110"
     CDS             2393..3118
                     /gene="aat"
                     /locus_tag="VFG22_08110"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HET6416248.1"
                     /translation="MPVYLLDKELTFPAPEGANEEGIVAVGGDVSPERLLAAYRNGIF
                     PWPARGYPLLWFSPDPRFALRPSKAHVSRSLRKLIRKGQLRITADLAFAEVITACAAV
                     PRPHQQGTWITPELQSGYLGLHRLGYAHSIEAWHDDRLVGGLYGVALGRTFAGESMFA
                     SEPDASKVAFATLLGHLASWGFRLVDCQVHTEHLARFGASMWPRQRFLSEWRAAVDEP
                     SRLAPWRLSMSPAEALAQLDAGA"
     gene            complement(3148..>4205)
                     /locus_tag="VFG22_08115"
     CDS             complement(3148..>4205)
                     /locus_tag="VFG22_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416249.1"
                     /translation="APFIGAGFAQLEWIRSARRTVAVALALGFAWALWVAPRLEPFVP
                     FGFGEPRLGHPFAVAEVIRANVEEPKILAPIQDSWPLMFAVPEAKFLIDGRVPFYGPE
                     FIRRVTNSFSDPAALNALVSEYGVDTIVLDHVRADQSAAVEYLSRSRDWVLGQVQDRH
                     SLFVRPERAPSMLPLQVIGAGYRVGRLLDSDVSDAQIEAEIRRISPHQSSDAIRGWAR
                     GLRALRPLARDGARAGIRMARTDAERDAAREAYRHLSEAAAVYPGFTSIELYRAMAAL
                     AACDESAAREALAWAAYSGETRETSLVAIELALRMDAESRRAAAESRILSLAGDARSA
                     EDPWVIAIARDREARCP"
BASE COUNT          829 a         1355 c         1322 g          699 t
ORIGIN      
        1 tggagcccga agaggaggaa gacgcgtaga catggctggc cccgtcatcg ataaagagct
       61 tcaagcaacc cttcgcaagg ccttcgaccg tgcggggctg atgcgccacg agtacgtgac
      121 gctcgagcac ttgcttctcg ccctaacgga ggatccgaaa gcgtccaagg cactgcacgc
      181 ttgcggggca gacatccgcc gtttgcgaaa gaagctcgag gaattcattc aggagcgcct
      241 ttcgcgcgtg ccagagagcg tcagcgtcga cccccgtcaa acgctcgcag tcgagcgggt
      301 actccagcga gccgcgattc acgcgatctc ttcggagatg aagacgatcg acggcagcaa
      361 cgtgctcgtc cagctgctca aggaagagga gtccttcgct gccttcatcc tccaggaaga
      421 aggggtgaac gcgctcgatc tcaagcgcta catcgcgcac ggagtcggca ctgacatcgt
      481 ccccggcgaa agcgacgcct acacggacac tgacttcgaa gacgacgaag gcgaaggcgc
      541 cgcgggtcgc gatcccctcg aggcgtttgc caccaacctg atcacggagg ccgccgaggg
      601 acgcatcgac cctttgatcg gccgcaatga agagctcgac cgcaccatcc aggtgctctg
      661 tcgccggcgc aaaaacaacc ctgttttcgt cggcgaacca ggcgtgggta agacggcaat
      721 cgccgagggg ctcgccctac gcatccacga aggcaaggtc ccagagatcc tcgaagatgc
      781 cgagatttac tcgctcgaca tgggcgccct gctcgcagga accaagtttc gcggccagtt
      841 cgaagagcgt ctcaaggcgg tcatgaaacg actgcaagag ctcgacaacg cgatcctctt
      901 catcgacgag atccatacca tcgtcggggc cggcgcgacg acgggcagct cgatggacgc
      961 ttccaacatt ctcaagcccg ccctggcgtc gggaaagttg cgatgcctag gctcgacgac
     1021 ctacgcggac ttcaagcatc tcgagcgcga ccgagccctt gcgcgcaggt ttcagaagat
     1081 cgaggtgctc gagcccacgg tcgcggacac catcgagatc ctcaagggcc tacgccagta
     1141 ctacgaagac caccatgacg tgaagtacga cgacgacgcc ctcgcggcag cggcgcagct
     1201 cgctcagaag cacatcgtcg agcgattctt gccggacaag gcgatcgacg tgatcgacga
     1261 agcaggctcc ctcgagcgga tgcgcccgga gggcgaacgc actcatcgca tcaccgtgaa
     1321 agagatcgag agggtcgtca gcaagatggc tcgtgtgccg gtcgaatcgg tgagcgctga
     1381 agaacgcgac cagctcaagg agctcgggcc cgagctccgc aaggtgatct tcggccagga
     1441 cgaagccatc gacaaggtcg tcagctcgat cactttggcg cgggccggcc tgcgcgcgga
     1501 caacaagccg atcgggtctt ttctctttgc gggcccgacc ggcgtcggca agacggagct
     1561 tgcccggcag ctcgcccgag tgatgggggt cgagcttctg cgcttcgaca tgagcgagta
     1621 cggtgagcga cactccgttt cgcgtctgat cggagcgccg cccggctacg tcggcttcga
     1681 ccagggtggc ctgctcacgg acgcggtgcg gaagcaccct cacgccgtgg tcattctcga
     1741 cgaaatcgag aaggcccatg gggagctgtt caacatcctc ctacaggtga tggaccacgc
     1801 caagctcacc gacaacaacg ggcgcgaggc tgatttccga aacatcgtgc tggtgatgac
     1861 gaccaacgca ggcgccttcg aagccacgga gaaggtcgtt ggttttggtg gcggcgacgg
     1921 acccagcgac ttcgccaagg gtcgcgcgaa gcaggcgatg gagcgcatct tcacgccgga
     1981 gttccgaaat cgcctcgacg cggtcgtcta ctttgccggg ctccgaaaag aggtcatccg
     2041 caaagtggtg gacaaagaaa tcggcttgct cagcgcgatg ctgagcgaga aacaggtgcg
     2101 gctggagctc gatgatgcgg cgcgcgactg gctcgccgac catggctacg atccccaaat
     2161 gggcgcccgt ccaatggcac gtctggtcga gcagaagctc aagaagcctc tggccgaggc
     2221 gatggtgttc ggtgacctca aagaaggcgg cacagcgcgg gccgcggtcc aggacggcga
     2281 aatcgtgctt tcattcgacg agaccgggcc gcccaccgtg cattgagcgg agagcgtcta
     2341 ggcaaggaac caccgccgag atgccgcttg cgctagcctg agccgttcgc cgatgcccgt
     2401 gtacctactc gacaaggagc tgaccttccc ggcgccggaa ggggcaaatg aagaaggcat
     2461 cgttgccgtc ggcggagacg tatctccgga acgtctcttg gcggcctatc gaaatggcat
     2521 cttcccatgg cccgcgcgcg gctatccgct gctttggttt tcgcctgatc cgcgcttcgc
     2581 gttgaggccg tcgaaggccc atgtctctcg gtccttgcgc aaactaattc gcaaggggca
     2641 actccgcata accgcggacc tggcttttgc cgaggtgatc accgcatgcg ctgcagtgcc
     2701 tcgcccacat cagcagggaa cctggatcac tccggagctc cagtcaggct acctcggact
     2761 ccaccgactc gggtacgcgc acagcatcga ggcgtggcac gacgatcgac tcgtcggcgg
     2821 cctctacggc gtcgcgctcg ggcgaacctt cgcgggcgaa tcgatgttcg cttccgaacc
     2881 cgatgcatcg aaggtcgctt ttgcgacctt gctcggacat ctggcgagct ggggttttcg
     2941 tctcgtcgat tgccaggtcc acaccgaaca tctggctcga ttcggcgcca gcatgtggcc
     3001 tcggcaacgt tttctttccg aatggcgcgc tgcggtcgac gaaccgagcc gcttggcgcc
     3061 ctggcggctg agcatgtccc cggccgaggc tctcgcgcag ctcgacgcag gtgcatgagc
     3121 ggcctcaacg cgctccgtgg atccccttca tggacagcga gcttcgcggt ctcgcgcgat
     3181 cgcgatgacc caggggtcct cggcgctccg cgcatcccct gcgaggctca gaatcctcga
     3241 ctcggcggcg gcccgtcggc tttctgcgtc catgcggagc gcgagctcga tggcgaccag
     3301 agaagtctcg cgcgtttctc ctgaataagc agcccaggcc agcgcctccc gcgccgcgga
     3361 ctcatcgcat gccgccaacg ccgccatcgc ccgatagagc tcgatcgaag tgaaaccagg
     3421 atagaccgct gcggcttcgc tgaggtgtcg ataggcctcg cgagccgcat cgcgctcagc
     3481 gtccgtccgc gccatacgta tgccggcccg tgcgccatcg cgcgcaagcg gtcgtagcgc
     3541 tcgaaggcct cgagcccatc ctcgaatcgc gtcggagctc tggtgcggcg atatgcgtcg
     3601 gatctcggct tcgatctgtg catccgaaac atcggaatcg agcagtcgcc ctacccgata
     3661 cccggctccg atcacttgga gcggaagcat cgagggggca cgctcgggac gcacgaacag
     3721 ggaatggcga tcctgaacct ggccgagcac ccagtcgcgg gagcgagaga ggtactcgac
     3781 cgccgcactc tggtccgctc ggacgtgatc gagcacgatc gtgtcgacgc cgtactccga
     3841 gaccagcgcg ttcagagccg ccggatcgct gaagctgttg gtcacccgtc gaatgaactc
     3901 cggcccatag aacggcacgc ggccgtcgat gaggaacttg gcctccggaa cagcgaacat
     3961 cagcggccag ctatcttgga tcggcgctag gatctttggc tcttcgacgt tcgcgcggat
     4021 cacttcggcg accgcgaacg gatgccccaa tctcggctcc ccaaaaccga acggcacgaa
     4081 tggctcgagg cgcggtgcaa cccagagcgc ccaagcgaac ccgagagcaa gcgcgaccgc
     4141 tacggttcga cgtgccgatc gaatccattc gagctgtgcg aagccggcgc cgatgaaggg
     4201 cgccg
//
LOCUS       DASRZF010000183         6984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3562095, whole genome shotgun sequence.
ACCESSION   DASRZF010000183 DASRZF010000000
VERSION     DASRZF010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 6984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6984
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3562095"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..1376)
                     /locus_tag="VFG22_08120"
     CDS             complement(108..1376)
                     /locus_tag="VFG22_08120"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6416250.1"
                     /translation="MTEIDDSERKAPDSGVSKAPHGLLGHTIDQRYRVESLLGEGGMG
                     LVYRVRHTQLNKPLAIKVLRRENTRDPEVLARFRREAESASAIGNEHIVDINDFGVLD
                     DGSTYFVMECLEGIDLIDAIDAAQRMPEARAIRIAMQVCRALGAAHDAGIIHRDLKPE
                     NVFLIRRNETDDFVKVLDFGIAKVANGPKRLTRAGEVLGTPHYMSPEQCEGDNVDHRT
                     DIYALGVLMYEMVTGHVPHDADTMMGILTKQMYEDPIPPKVRAPQVSDQLEQLIMRCL
                     EKKPEQRYQTMHEIEDDLRRVREGQEPVGPDTVTLTPTRPPRPARARVSPLYLGGLGL
                     AVLALVGTLAIRAANQRPQVGQMGHLVPGAEPSEARATESIEEPPVTASGDAPKGDVE
                     TPSPKRARRKVRQASKAPSQHDQAILDPWK"
     gene            complement(1455..4127)
                     /gene="ppdK"
                     /locus_tag="VFG22_08125"
     CDS             complement(1455..4127)
                     /gene="ppdK"
                     /locus_tag="VFG22_08125"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HET6416251.1"
                     /translation="MTLLYPFDAELDSDVRLQLLGKKAAGLQEMSLLGIPVPPGFTIT
                     TEVGNQFRKTARWPDGLDAAIEQALRSIEARMGRGFGAADQPLLLSVRSGAPVSMPGM
                     MDTLLNVGLSEGTLSGLAAQRGGERFALDSYRRLLQMYGAIVRGIGADRFQRLLSDLK
                     TKLGYPRMLDSEIPVVALEELVAAYLSLIDEASGSPFPQDVDTQLWDAIGAVFASWDN
                     PRATRYRRMQRIDRASGTACIVQAMVFGNAGARSGSGVAFTRNPSNGDKQLYGEFLPN
                     AQGEDVVSGMRTPVALTKSAAAPGKEGFTLEASLPEAFEAISSYCASLEKHFGDMQDI
                     EFTIEEGLPYLLQTRTAKRAAKAAVRIAVDMVKEGVLSKEDALLRVDARSLEQLLRAR
                     LPSDAELSAQGVEALTVGLPAGPGAASGRIVFGPDEAVRWAAEGQDVILIRQETRADD
                     IHGMKAAKGVVTAAGGMTSHAAVVARGLGKCCVVGCADLEIDVERRTVRLAGAYSSEP
                     LREGDLLTLDGTNGKVYSGALDLVASATVDELQELMSWADEVRRMQVYAEADTPQTAR
                     AALSYGAQGVGLCRTERMFFAQDRLFAMRCALLSVEDDQRSKWLSDLGRAQEEDFAEI
                     FRAMDGRPVTIRLLDRALGEFLPEDETALRAVADALDLELDEVITAAARHRESNPAFG
                     HRGVRAGLTIPGLYEMQLRAMLVAARTCTDAGVPVELEVLIPMVSYSSEVEAVRAVLA
                     RVRDQVFVESTSLFTCRVGVMIELPRACLIADELAELTDFFSFGGNDLTQTTLGISRE
                     DAGRFLPSYLNELGMMENDPFNCLDARVAELMQIALERAKRRNQSLIAGLCGDQGGSP
                     ATIAICERLGLDFVSAPLSQIPGARLAAAQARLR"
     gene            complement(4105..6213)
                     /gene="glyS"
                     /locus_tag="VFG22_08130"
     CDS             complement(4105..6213)
                     /gene="glyS"
                     /locus_tag="VFG22_08130"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HET6416252.1"
                     /translation="MSSEFLLEIGTEELPASFVTHALRSMKSNAVELLGQARLGTDAF
                     EVETLGTPRRLAIRVRGLAQHQPDRDETVMGPPWSAAFEANGTPKKAAVGFAKKHGVA
                     VGDLRKQTTDKGDYVSVRVHEAGKGTHEVLREILPVLCQRITFPKSMRWGAGEIAFGR
                     PIHWIVSLLESDVVGFEFAGVSAGRASRGHRFLSPASFDLESADRYEAALEDAHVLVD
                     LLRRKARMMDDLVASAKSLGGVLEPDAFLADECVSLVEEPFIVPGSFDESYLELPDAV
                     VISVMRDHQRYFAVRASKGGPLLPAYLNVVNTAEDPETITKGNDRVLRARLADARFFV
                     EEDRKYPLASRLEKLESVVFQTKLGSMGAKVRRLQSNARALAERAGADVDACVAAARL
                     CKADLETLIVFEFPELQGEMGRWYALREGIDPSVADAIRDHYRPQGADDVVPDQVVGA
                     VVAVADRIDTLVGCFGIGLVPTGSADPFALRRAALGIIRIALEGPIDVDLHELIAQSR
                     TAYEGDADLIAAEDLRASLDDFFRGRIRGVLKDSHGGDLVDACLGAWDGKSLRDLRSR
                     IEAVSELGTTPEFEALAVAFKRAFNITKESARGAVDRSLLEDGPETALAERFWTVRDQ
                     IREATGERRYMDALKLVAKELGAPIDRFFEEVFVMVDDPRVRDNRLRLLGEIADTVNG
                     IAHFHQLGTKLQRRDPSLSV"
     gene            complement(6273..>6984)
                     /locus_tag="VFG22_08135"
     CDS             complement(6273..>6984)
                     /locus_tag="VFG22_08135"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HET6416253.1"
                     /translation="YQVILKPSPLDIQELYLESLRALGTDPSRHDIRFIEDDWESPTL
                     GAWGLGWQVWLDGLEISQFTYFQQCGGIDCKPISGELTYGLERIAMYLQDVDDVYELQ
                     WAPGIRYGDLYKRAEWEWSTYNFEQADIELQKNLFGEYEKQVAMLLGLSDTKGKGGAL
                     QFDREPLERLALPAYDAVIKCSHYFNVLDARGAISVTERQRYIGRVRILARAVAESWY
                     AQREALGFPLLDELAAAQ"
BASE COUNT         1241 a         2248 c         2182 g         1313 t
ORIGIN      
        1 caagaacccg agagcgaaac ggatgggcgc gccctgcggg tcgaatcgcg accccatggg
       61 gcgccaccct agccgttcac cctagagggg gcaactggca gcggggccta cttccaggga
      121 tccaggatgg cctggtcgtg ctgcgaaggc gctttagagg cctgccgcac cttgcgccgc
      181 gccctcttcg gagatggcgt ttcgacatcg cctttcgggg catcgcctga cgcggtgacc
      241 ggcggctcct cgatcgattc cgtcgctctc gcttccgagg gttcggcgcc ggggactagg
      301 tgacccattt gcccaacctg ggggcgctgg ttcgctgctc tgatcgccag ggttcccacg
      361 agcgcgagca cggccaaacc gagccctccg agatagagtg gtgagacacg ggcccgggcg
      421 gggcgcgggg gccgcgtggg tgtcaaagtc accgtgtccg ggccgacggg ctcctgtcct
      481 tcgcgaacac gtcgcaggtc gtcctcgatc tcgtgcatcg tctgatagcg ttgctcaggc
      541 ttcttttcga ggcagcgcat gatcagctgc tcgagctggt ccgagacctg aggcgcccgc
      601 accttcggtg gaatcgggtc ctcgtacatc tgtttggtga gtatgcccat catggtgtcc
      661 gcgtcgtgcg gcacgtgacc ggtcaccatc tcgtacatca atacgccgag cgcgtagatg
      721 tccgtccggt ggtcgacgtt gtctccttcg cactgctccg gcgacatgta atgaggcgtg
      781 ccgagcacct cgccggcgcg cgtgaggcgc ttgggtccat ttgcgacctt ggcaatcccg
      841 aagtcgagta ccttcacgaa gtcgtcggtc tcgttgcgcc ggatcaagaa cacgttttcg
      901 ggcttgaggt cgcggtgaat gatcccagca tcgtgcgcgg cgccgagcgc gcggcagacc
      961 tgcatggcga ttcgaatggc gcgcgcttcg ggcatgcgct gcgcggcgtc gatcgcgtcg
     1021 atcaggtcga ttccttcgag gcactccatc acgaagtatg tcgagccgtc gtcgagcacg
     1081 ccgaagtcgt tgatatcgac gatgtgctcg ttgccgatcg ccgaggcgct ctccgcctcg
     1141 cggcgaaatc gggctagcac ctcgggatcc cgagtattct cgcggcggag cactttgatt
     1201 gcgagaggct tgttgagctg cgtatgcctg acgcgataca ccagccccat tccgccctcg
     1261 ccgagcaagg actcgacccg ataccgctga tcgatcgtgt gaccgagcag cccatgcggg
     1321 gccttcgaca cccccgaatc aggcgccttc cgctctgagt cgtcaatctc agtcacgccg
     1381 ttgggcatac agtctcacca aaatcttcca ggaatctaca ttccaacacg ccccggcgcg
     1441 gccagcaacc ccttttatcg gaggcgcgct tgggccgcgg ccaggcgggc accgggaatc
     1501 tgcgagagag gggccgagac gaagtccagc cccagacgct cacaaatggc gatggtggcg
     1561 gggctcccgc cctggtcgcc acagagccct gcaatcaggc tctgattgcg gcgcttggcc
     1621 cgttcgaggg cgatttgcat gagctcggcg acccgggcgt cgaggcagtt gaaagggtca
     1681 ttttccatca tccccagctc gttgaggtag cttggaagaa agcgcccggc gtcttcgcga
     1741 ctgatgccga gagtcgtctg cgtgaggtcg ttgcctccga aggagaagaa atccgtgagc
     1801 tccgccagct catcggcgat cagacaagcg cgcgggagct cgatcatcac gccgactcgg
     1861 caggtgaaga ggctcgtgga ctcgacgaag acttgatcgc ggacgcgggc caatacggcg
     1921 cgaacggctt ccacctcgga gctgtacgag accatgggaa tcaagacttc gagctcgact
     1981 gggacgccgg cgtccgtaca cgtgcgcgcg gcgaccagca tcgcgcgaag ctgcatttcg
     2041 tagaggcctg gtatcgtgag gcccgcgcgt acgccccgat gaccaaatgc cgggtttgat
     2101 tctcgatgcc gggcggctgc cgtgatgact tcgtcgagct ccaggtcgag cgcgtctgca
     2161 accgctctca acgccgtttc gtcctctggc aggaactccc cgagcgcacg gtcgagcaaa
     2221 cgaatcgtga ccggccttcc gtccatcgcg cggaagatct cggcgaagtc ctcttcttgc
     2281 gcgcgtccga gatcagacag ccatttgctt ctttggtcgt cttcgaccga gagcaacgcg
     2341 cagcgcatcg caaacagtcg gtcttgagcg aagaacatcc gctcggtccg gcagaggcct
     2401 accccctgcg caccgtagct caatgcagcc cgcgcagtct gcggcgtatc ggcctcggcg
     2461 taaacttgca ttcgacgaac ctcatcagcc caggacatca gctcctgaag ctcgtcgacg
     2521 gtcgccgatg cgactagatc gagggcgccc gagtagactt tgccattcgt gccatcaagg
     2581 gtcagcaagt caccctcccg caacggctcc gatgagtatg cgcccgcgag tcgcaccgtg
     2641 cggcgctcga cgtcgatttc gagatcggcg caccccacca cgcagcactt gccgagtccg
     2701 cgagccacga ccgcagcgtg cgaggtcatt cccccggctg cggtgaccac ccccttggcg
     2761 gccttcatcc cgtgtatgtc gtcggccctg gtctcttgac gaatcaggat tacgtcttgc
     2821 ccttctgcag cccagcgaac ggcttcgtcg ggcccgaaca cgatgcggcc ggaagccgca
     2881 ccaggccccg cagggaggcc caccgtgagc gcttcgaccc cctgcgcact cagctcggcg
     2941 tcgctcggaa gtcgcgccct gaggagctgc tcgagcgagc gagcgtcgac ccgaagcaat
     3001 gcgtcctctt tgctcaggac accttccttg accatgtcca ccgcgatacg gacggccgcc
     3061 ttggcggctc gtttcgccgt ccgggtctgc aacagatacg ggagcccttc ctcgatggtg
     3121 aactcgatat cctgcatgtc accgaagtgc ttttcgaggg aagcgcaata ggatgaaatg
     3181 gcttcgaatg cttcggggag cgatgcctcc aaagtgaatc cttcctttcc tggggcggcc
     3241 gcactcttgg tgagcgcgac gggagtccgc atccctgaga ccacgtcttc gccctgtgcg
     3301 tttggaagga actctccgta gagctgcttg tctccgttcg acggatttcg ggtgaaggcg
     3361 actccagaac cgctccgtgc gccggcgttc ccaaaaacca tcgcctgcac gatgcacgcg
     3421 gttccgctgg cccggtcgat ccgttgcatg cgacggtatc gcgtcgcccg aggattgtcc
     3481 caggacgcga acaccgcgcc gatggcgtcc cagagctgcg tgtctacgtc ctgcggaaac
     3541 ggcgaaccag atgcttcgtc gatcaaagag aggtacgcgg cgaccaactc ctcgagtgcc
     3601 acgacgggaa tctcggagtc gagcattctc ggatatccga gtttggtctt gaggtcactc
     3661 agcagtcgct ggaaccggtc tgctccgatc ccccgcacga tggcgccgta catttgcagc
     3721 aggcgacggt atgaatcgag agcgaaacgc tcaccgccgc gctgcgccgc gagcccgctc
     3781 agcgtccctt cgctgaggcc gacgttcaag agagtgtcca tcatccctgg catggacacg
     3841 ggcgccccgg atcggaccga aagaagcaat ggttggtcag cggctccaaa gcctctgccc
     3901 atccgcgcct cgatcgatcg cagtgcctgc tcgatcgccg cgtcgagacc atccggccat
     3961 ctggcggtct tgcggaactg gttgccgacc tcggtcgtga tggtgaatcc cgggggcacc
     4021 ggaattccga ggaggctcat ctcctgcagg ccggccgcct tcttcccgag gagttgcagg
     4081 cggacgtccg agtccagctc tgcgtcaaac ggataaagaa gggtcacggc gctgcaactt
     4141 tgtcccaagc tggtggaagt gcgcaatgcc attgaccgta tctgcgattt ccccgagtaa
     4201 tcgcaatcga ttgtcgcgca ctcgcgggtc gtcgaccatg acgaacacct cttcgaagaa
     4261 tcgatcgatc ggtgcgccga gctccttggc gacgagcttc aacgcatcca tgtagcggcg
     4321 ctcacccgtt gcttctcgga tttgatcgcg aacggtccag aatcgttccg ccagcgcggt
     4381 ttcgggtccg tcttcgagaa gcgagcggtc gacggcgccc ctcgcgctct ctttggtgat
     4441 gttgaaggcg cgcttgaatg caacggcgag cgcttcgaat tccggggtgg tgcccagctc
     4501 cgagaccgct tcgattcggc tccgcagatc gcgcagcgac ttgccatccc aggcgccaag
     4561 gcaggcatcg accaggtcgc caccgtgcga atccttgaga acgccgcgga ttcggccgcg
     4621 gaagaaatcg tcgagggagg cgcgaagatc ctctgcggcg atgagatcag cgtcgccctc
     4681 gtatgcggtg cgcgattggg cgatcagctc atgaaggtcg acgtcgatgg gaccttcgag
     4741 cgcgatgcga atgatcccga gtgcagcgcg tcgcagagca aacgggtcgg ccgagccagt
     4801 aggaacgagg ccgatgccga agcatcccac caaggtgtcg atccggtcgg ccacggcgac
     4861 tacggcgccc accacttgat cagggaccac gtcgtccgcg ccttgaggcc ggtagtgatc
     4921 gcgaatcgca tcggcgaccg aggggtcgat tccttcgcgc agagcgtacc aacgacccat
     4981 ctcgccctgg agctcgggaa attcgaatac gatgagcgtc tccaggtccg ccttgcagag
     5041 ccgggctgcc gcgacacaag cgtcgacgtc ggcgccggct cgctcggcga gcgcacgggc
     5101 gttcgattgg agtcgtcgga ccttcgcgcc catcgagcct agcttcgtct ggaatacgac
     5161 cgattcgagc ttctcgagcc tgcttgcgag cgggtacttg cgatcctctt cgacgaaaaa
     5221 gcgggcatcg gcgagccggg cgcgaaggac ccggtcgttg cctttcgtga tggtctccgg
     5281 atcttcggcg gtgttgacta cattcaagta cgcagggagc agaggcccgc ccttggacgc
     5341 ccgaacggcg aagtatcgct ggtgatcgcg catgaccgag atgacgaccg cgtcaggtag
     5401 ctcgagatac gactcgtcga agctcccggg gacgatgaag ggctcttcga cgagcgaaac
     5461 acattcgtcg gccagaaacg cgtcaggctc gagcactcct cccagcgatt tcgcggaggc
     5521 gactagatcg tccatcatgc gagccttgcg ccgcagcaag tcgaccagga cgtgcgcgtc
     5581 ttccagagct gcttcgtacc gatccgcact ctcgaggtcg aagctcgcag gtgagagaaa
     5641 tcgatgcccc ctgctggcgc gaccggcact cacgccagcg aactcgaaac cgacgacgtc
     5701 ggactcgagc aaggagacga tccagtgaat cggcctgccg aacgcgattt caccagcgcc
     5761 ccagcgcatc gatttcggaa atgtgattcg ctggcagagc accggaagga tttccctcag
     5821 cacttcgtgg gtgcctttcc ccgcctcatg cacgcgcacg ctcacgtagt cgcccttgtc
     5881 ggtcgtttgc ttgcgaaggt ctcccaccgc gacgccgtgc ttcttggcga atccgacggc
     5941 ggccttcttt ggcgtgccat tcgcctcgaa tgccgcgctc cagggtggcc ccatcaccgt
     6001 ctcgtctcga tcaggctggt gctgcgcaag tcctcggacg cggatcgcga gccttcgagg
     6061 cgtgcccaag gtctccacct cgaacgcatc ggttcctagc cgggcctggc ccagtagctc
     6121 cactgcgttc gacttcatcg agcgcaaggc gtgggtcacg aaggatgcag gcagctcctc
     6181 ggtcccgatc tccagcagaa actccgatga catggagtgg cgttgctacc agaccggatc
     6241 cagggacgca acggggcggg gctagaaacg ggtcattgag ccgctgcgag ctcgtccagc
     6301 agtgggaagc cgagggcttc gcgctgcgcg taccacgact cggcaaccgc tcttgcgagg
     6361 atcctcacac ggccgatgta acgctggcgc tcggtcacgc tgatcgcgcc ccgggcatcg
     6421 agtacgttga agtagtggct gcacttgatg accgcatcgt aggcggggag cgccaatcgc
     6481 tcgagcggct cacggtcaaa ctgcagtgcg ccgcccttcc ccttcgtgtc gcttaggccg
     6541 aggagcatcg cgacctgctt ctcgtactcc ccgaagaggt tcttctgcag ctcgatatca
     6601 gcctgctcga agttgtaggt gctccactcc cactcggctc gcttgtagag gtcgccgtag
     6661 cggatgccag gggcccattg gagctcgtac acgtcgtcga cgtcttgaag gtacatggcg
     6721 atgcgttcga gcccgtaggt cagctctccg ctgatcggtt tacagtcgat gccaccgcat
     6781 tgctggaaat aggtaaattg actgatctcg agcccgtcga gccagacctg ccagccgaga
     6841 ccccatgcgc ccagggtcgg tgactcccag tcgtcctcga tgaagcggat gtcgtggcgg
     6901 ctcgggtccg taccgagcgc ccggagcgac tctaggtaga gctcctggat gtcgagcggc
     6961 gagggtttga gaatcacttg gtac
//
LOCUS       DASRZF010000184         2631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2878596, whole genome shotgun sequence.
ACCESSION   DASRZF010000184 DASRZF010000000
VERSION     DASRZF010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2878596"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="VFG22_08140"
     CDS             complement(<1..662)
                     /locus_tag="VFG22_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416254.1"
                     /translation="MTKQALLRILAVVCGLLVFQGCKVRTGVVDTKDPVVVLEYGVGP
                     RTPLRYAIDEGSTTTTTLEISTSSMMTTTSSGEKLTKGPSLRFVVSSGPAIKLPTGNV
                     RLDIRIVDAEAVLPPGFEPQASRDFSQSAALLRGVGGWIEVDDRGNIVRSELDQAAKN
                     PKVPTRLLMTIVQERTSLARVVFPAEPVALGARWEARKPLKLYGFEIQQTDRYTLRDR
                     SG"
     gene            826..1293
                     /locus_tag="VFG22_08145"
     CDS             826..1293
                     /locus_tag="VFG22_08145"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET6416255.1"
                     /translation="MAIIGSLMKTEMITVKPGDSVADAARTMSQNHVGAVLVVEGGVM
                     QGILSERDVVSRVVALGKDPSTTLVSEVATAEVVTVDVGTRVRKCAEMQFALDVRHLP
                     VTENGKPVGILSSRDFLAYVAGGLERIIDKLDYEETLGEGVDPYDHLGGSYGT"
     gene            1466..1759
                     /locus_tag="VFG22_08150"
     CDS             1466..1759
                     /locus_tag="VFG22_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416256.1"
                     /translation="MRERKSSTSDLHAQISDALRFGRVAEALDLYERIERRRPDEPRW
                     SHRKGDLLRRMGRDVDAVMAYRRAAELYAAKGFEARATATAKLMRQILARQPA"
     gene            1896..2315
                     /gene="mscL"
                     /locus_tag="VFG22_08155"
     CDS             1896..2315
                     /gene="mscL"
                     /locus_tag="VFG22_08155"
                     /inference="COORDINATES: protein motif:HMM:NF001843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HET6416257.1"
                     /translation="MLKEFKEFALKGNVMDMAVGIIIGAAFGKIIASLVGDVLMPVVG
                     LFTGGVDFSQSYVLLGDGSYESLAAAEAAGAAVLKYGAFIMSVFDFVIIAFVIFMMIK
                     GMNSLRRKEEKAEVAAPPPPPKEQVLLEEIRDLLAKQ"
     gene            complement(2364..>2631)
                     /locus_tag="VFG22_08160"
     CDS             complement(2364..>2631)
                     /locus_tag="VFG22_08160"
                     /inference="COORDINATES: protein motif:HMM:NF024592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4010 domain-containing protein"
                     /protein_id="HET6416258.1"
                     /translation="GDPGLYVIATISGLTDVDAITLSTANLIEAGRLDPGSGWRAIVL
                     ASLSNLVFKGVLGSALGGRALMKHLAPLFLTSIAAGITVLLLWH"
BASE COUNT          556 a          780 c          804 g          491 t
ORIGIN      
        1 ttgccgctcc tgtcccgaag cgtgtaccgg tcggtctgct gaatttcgaa gccgtagagt
       61 ttcagcggtt tgcgggcctc ccaacgagcg cccagtgcca ccggctcggc aggaaaaacg
      121 acacgtgcca gcgacgttcg ctcttgaacg atcgtcatga gcaaccgcgt gggcaccttc
      181 ggattcttcg ccgcctggtc cagctcgctg cgcacgatgt tcccgcggtc gtcgacctcg
      241 atccaaccac ctacgcctct gaggagcgca gcgctttggc tgaaatcacg cgaggcttgc
      301 ggttcgaatc caggcggcag caccgcctcg gcatcgacga ttcgaatgtc caaacggacg
      361 ttgcccgtcg ggagcttgat cgcaggaccg gaggacacca cgaagcgaag gctcggcccc
      421 ttggtcaatt tctcgccact gctagtcgtc gtcatcatcg acgaagtcga gatctccaag
      481 gttgtagtgg tggtggaacc ctcgtcgatt gcgtagcgaa ggggagtcct cggcccaacg
      541 ccatattcca gcaccacgac cggatccttc gtgtcgacta cacctgtgcg aaccttgcac
      601 ccctggaaga cgagcagccc acatacgacg gccaatatgc gcagcaatgc ttgcttggtc
      661 atgaccgccc tccgttcatc gcacccgaag acaccatcct catcaccgca ccctctctgg
      721 tttggatcca cgagagactt aagcacggaa cgcgtcgaca accaagcgaa tgcgtcccat
      781 caattcgtcg ggtaggctag cccgtcactg aaaggaggcg accccatggc aattatcggt
      841 tcgttgatga agacggagat gatcacggtg aagccgggcg attccgtcgc agacgcggct
      901 cgaaccatgt cccagaacca cgtaggcgcg gtactcgtcg tcgaaggcgg agtgatgcaa
      961 ggcattctct ccgagcgcga tgtagtcagc cgagtcgtgg ccctcgggaa ggatccgagc
     1021 acgacgctgg tttcggaagt cgcgacggca gaggtcgtca cggtagatgt cggtacccgg
     1081 gtgcgcaaat gcgcggagat gcagtttgca ttggatgtgc gccacctgcc ggtgacagag
     1141 aacggaaagc ccgtgggtat tctctcgagc cgcgactttc tcgcatacgt ggccggaggt
     1201 ctcgagcgca tcatcgacaa gctggactac gaggagacgc tcggcgaagg cgtggaccca
     1261 tacgaccacc tcggcggatc ctacggcact tgaacgagcg cgattacaaa cgcctgaaat
     1321 gacggtattt ttcatcatgg accctgaccg caggatcaga tgaacgaata attacgtata
     1381 gacgcagggc tgaaacgggt gttttctaat cgtcctcccc gcgtcgcgtt gtgcgatgca
     1441 acagatgcaa gtcttggttg ggcctatgcg cgaacgcaag agcagtacct cggatcttca
     1501 tgcgcagatc agcgacgcac tccgtttcgg gcgtgtcgct gaagcgctcg atctctacga
     1561 gcggatcgaa agaaggaggc ccgacgagcc ccgatggtcg catcgaaagg gcgacctgct
     1621 gcgccgaatg ggcagggacg tcgacgcggt gatggcctac cggcgcgccg cagagctcta
     1681 cgccgccaaa ggcttcgaag cgcgcgctac ggctaccgcg aaactgatgc ggcaaatcct
     1741 ggcccgccag ccggcgtagt cgccaccaag ctcggcgtca tattcgctgg gaaacggggg
     1801 tcacgtgctt ggctgcgcgc agcggttgcg gccctgccgg gatccatgca tacgagcacg
     1861 cctccgtccg aggcaatgaa agggaagaga aagagatgct caaagagttc aaagagttcg
     1921 ccctgaaggg caatgtgatg gacatggcgg tcggcatcat catcggagcc gcctttggta
     1981 agatcatcgc ttcgctggtc ggcgacgttc tcatgccggt agtcggactc ttcaccggcg
     2041 gcgtcgattt cagccagagc tacgtcttgc tgggtgacgg cagctatgaa tcgcttgcgg
     2101 ccgcagaagc agcgggcgcc gcagtgctca agtacggtgc tttcatcatg agcgtgttcg
     2161 acttcgtcat catcgcattc gtgattttca tgatgatcaa agggatgaac tctctacgtc
     2221 gtaaggaaga gaaagcggaa gtggccgctc cccctccccc gcccaaggag caggtcctct
     2281 tggaagagat ccgcgacctc ctggccaagc agtagcagcg cgagggacgg ttacccggaa
     2341 ggccccatct cggatcgttc ccgtcaatgc caaagcagga ggactgtgat ccctgccgcg
     2401 atcgaagtga ggaagagggg cgcgagatgc ttcatcagtg ctcggcctcc aagcgcgctg
     2461 ccgagcacgc ctttgaagac caagttggag aggctagcga gcacgatggc gcgccaaccg
     2521 gaccccggat cgaggcgacc cgcctcgatc aaattggcgg tggacagtgt gatcgcgtcg
     2581 acgtcggtga ggcctgagat tgtcgcaatc acatagagac ctggatctcc a
//
LOCUS       DASRZF010000185         3440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2534591, whole genome shotgun sequence.
ACCESSION   DASRZF010000185 DASRZF010000000
VERSION     DASRZF010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2534591"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VFG22_08165"
     CDS             <1..257
                     /locus_tag="VFG22_08165"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF013398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HET6416259.1"
                     /translation="YVPGDRILGLGKLPRLVEAYARRFQIQEDLVRDIAREMMESGHA
                     RGVRVQSEARHLCMISRGPQSAASVTRTEHALGCLGDESE"
     gene            411..1187
                     /locus_tag="VFG22_08170"
     CDS             411..1187
                     /locus_tag="VFG22_08170"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET6416260.1"
                     /translation="MLGFFVESKPTKSLTLGDLTQHIEPRRLVVLEPYEHEQVAFTAL
                     PLEQVLDEAYGPSWRTHEAILFTCLDGYEPTIPVRRVLEHRAFLAIDRPGDAGFTVLK
                     DEEGTRKRIDLGPFYVVWENLDNARVRNEGDYGWPYQVVRIDLVSFRSRFREMAPPPK
                     AGEEVVAGFDAFVAHCSKCHTINGHGGAIGPELNHPANPTEYMKDEWLRKWIDDPTSM
                     RRAPRMPPLNPELPDRARIIDEIVAYLDVMASHKIAPDGP"
     gene            1184..1846
                     /locus_tag="VFG22_08175"
     CDS             1184..1846
                     /locus_tag="VFG22_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416261.1"
                     /translation="MTYAELYGSFIQHPLLLWAATILGLAAALSRTGLSSTVRAYCVV
                     LATMSLLDAWLTSNQVLGIGSLTGAAASFVPLAFVLLGDFRYFLFVEIAKPDGALSVS
                     AGALIRACVWTLLVPILSQLAMVALGSNDSRVLFFVYESLFVLLAVGISRLYLPRRSR
                     ALLWTRRVTNFVLVYYALWATADALILTTGSDAGYLLRILPNVFYYGGLVPAIAWAGP
                     RP"
     gene            complement(1848..3230)
                     /locus_tag="VFG22_08180"
     CDS             complement(1848..3230)
                     /locus_tag="VFG22_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HET6416262.1"
                     /translation="MVYQAGVQRDRSRRAQSAIVAIVFSICVGCGGSSADPRGGGGAA
                     GGGGGTGGAGGNPECTSSEQCLAPEVCEPVTQTCVAPLISCTSQTECTGGTYCLTPSN
                     VCVPSAVGTSCAGPDNCNGECLNGFCGCSGVAHERQLESSPLDIYLVLDRTGSMGTDC
                     SYVAGSSPPVSSKACFATYALADYLINVSPSVDTSLAFNVMSLSNDCSGASYDPALIA
                     STSLPVASDSALIQRISDENFSGGYGTRIEGALRDIANYTVNHQAAGREMIGVLITDG
                     DATNCQTNNSTLAGIIEDHLTDTGLRTFIIGMNGANEDKLEELAIAGGADPHDDFCGG
                     VAPPCHYWNVGDASGDVLASALQAISQQAVPLPCEIDVTGLTAPEGEVLDYGRVNVTL
                     TEGTSVTTIPQAANEAACPTDQPAWYYDSPSAPSKIFLCEGACATVSAAGDGAELNVV
                     AGCTNTIVVQ"
BASE COUNT          617 a         1059 c         1068 g          696 t
ORIGIN      
        1 cctacgtgcc gggagaccga attctcggac tcgggaagct gccgcgtctc gtcgaggcat
       61 acgcgcgacg tttccagatt caggaggacc tcgtgcgcga catcgctcga gagatgatgg
      121 aatcgggcca tgcgcgaggc gtacgggtgc aatccgaagc ccgacacctg tgcatgatca
      181 gtcgcggtcc tcagagtgcc gcgtcggtca cccgcaccga gcacgcactc ggatgcctcg
      241 gcgacgagag tgaatgagcc ttgcccatgg gcgtggctag agccccggtg aaaaacgcta
      301 gctcaggccg gcgggctata gtcctggcca tcgcgtgcgc ggcgatagcg tacggcgggt
      361 tggcttgtgc ccgcggcgac cgaaattcgg caagcgacga ggagcgccga gtgctcgggt
      421 ttttcgtcga gtccaagccg acgaaatccc tcacgctcgg agacctgaca cagcacatcg
      481 agccccgccg cctcgtggtg ctcgagccgt acgagcatga gcaggttgca ttcaccgctt
      541 taccactcga gcaggtgctc gacgaagcct atggaccttc gtggcgaaca cacgaagcga
      601 ttctatttac ttgtctcgac ggatatgaac caacgatccc cgtgcgacgc gtattggaac
      661 atcgggcatt tctggcgatc gatcgacccg gcgatgccgg attcacggtc ctcaaggacg
      721 aagaggggac gagaaagcga atcgacctcg gacctttcta tgtcgtttgg gagaacctgg
      781 acaatgcgcg agtacgcaac gagggtgact acggttggcc ctaccaggtc gttcgaatcg
      841 acctggtctc gtttcgatcg cgatttcgcg agatggcgcc gccccccaag gccggcgaag
      901 aagtcgtcgc aggcttcgat gcctttgtcg ctcactgttc gaagtgccac acgatcaacg
      961 ggcacggagg cgcgatcggc ccggagctga accacccggc caaccccacc gaatacatga
     1021 aggacgagtg gctccggaag tggatcgacg atcccacgag catgcgccgc gctccacgca
     1081 tgccccctct gaatccggag cttccagacc gcgcgcggat catcgacgag atcgtcgcgt
     1141 atctggacgt gatggcgtca cacaagatag cgccggacgg tccgtgactt acgcggagct
     1201 ctacggttcg ttcatccagc atcctctgct cctctgggcg gcgacgatcc tcgggcttgc
     1261 ggccgctctg tcgcggactg gcctctcgag cacggtgcgc gcgtattgcg tcgtgctcgc
     1321 aaccatgtcg cttctcgatg cttggctcac cagtaaccag gttcttggaa tcggttcatt
     1381 gaccggggcg gcggcctcat ttgttcctct ggctttcgtg cttctcggcg atttccggta
     1441 cttcctcttc gtcgaaatcg caaaacccga tggtgcgctc tcggtgagcg ctggagcttt
     1501 gatacgagcg tgcgtctgga cgctgctcgt gcccatctta tcgcagctgg cgatggtcgc
     1561 gttgggctcg aacgattctc gggtcctgtt cttcgtctac gagtcgctgt tcgtattgct
     1621 cgcagtcggc atcagtcgcc tttacctgcc ccggcgcagc cgcgcgcttc tgtggacacg
     1681 gcgggtgacg aattttgtcc tcgtatatta tgccctgtgg gctacagccg atgcgctcat
     1741 cctcacaacg ggttcggatg cgggttatct cctgcgtatc ttgccgaacg ttttctacta
     1801 cgggggcctg gtgccagcga tcgcctgggc tgggcctagg ccctagctca ctgcacgacg
     1861 atcgtatttg tgcagccggc caccacgttg agctccgctc cgtcacccgc agcgctcacc
     1921 gtcgcgcaag cgccttcgca gaggaagatc ttgctgggcg cactcgggct atcgtagtac
     1981 caggccggct gatcggtcgg gcatgcagcc tcgttcgccg cctgcgggat ggtggtgacg
     2041 cttgtaccct cggtgagcgt cacgttgacc cggccatagt cgaggacttc cccctcggga
     2101 gcagtgaggc cagtcacgtc gatctcacag ggcagtggta ctgcctgctg ggaaatcgct
     2161 tgtagagcgc tcgccagcac gtcgcccgag gcgtcgccca cgttccagta gtggcatgga
     2221 ggagccacac cgccgcagaa gtcgtcgtgt ggatcggcac caccggcaat ggcgagctcc
     2281 tcgagcttgt cctcgttggc gccattcatc ccgatgatga aggttcgaag gcccgtgtcc
     2341 gtgaggtggt cttcgatgat cccggcaagg gtggaattgt tggtctggca gttggtggcg
     2401 tctccgtcgg tgatgagaac gccgatcatc tcgcgaccgg ccgcttgatg gttgacagtg
     2461 tagttggcaa tgtcccgaag cgcgccttcg atccgggtac cgtatccacc actgaaattt
     2521 tcgtcgctta tgcgttggat gagggcgctg tccgaggcga ctggcagcga ggtgctcgca
     2581 atgagcgcag ggtcatagga cgcgccgctg caatcgttcg acagtgacat gacgttgaat
     2641 gcaagactcg tgtcgacgga aggagatacg ttgatgaggt agtcggccag agcataggtc
     2701 gcgaagcatg ccttcgagct caccggcggc gagcttcccg cgacgtaaga gcagtcggtg
     2761 cccatgctcc ccgtacgatc gagcaccagg tagatgtcga gcggactgct ctcgagctgt
     2821 cgttcgtgcg caacaccgct gcacccgcaa aaaccgttga ggcattctcc attgcagttg
     2881 tccggacctg cgcaggatgt gcccaccgcg gatggcacgc agacgttcga tggcgtcagg
     2941 cagtaggttc cgccggtaca ctcggtctga ctcgtgcacg agatgagggg cgcgacgcag
     3001 gtctgcgtga cgggctcgca gacttcaggg gccaggcact gctcgctgct cgtgcactct
     3061 gggttgcccc ctgcgcctcc ggttccgccg ccgccgcctg cagctccgcc tccgccccga
     3121 gggtccgcgc tcgaacctcc gcagcccacg caaatcgaaa agacgatcgc aacgatagcg
     3181 ctctgcgccc ttcgcgagcg atcccgctga accccagcct ggtagaccat actcaacagg
     3241 atagcgactc agaaatgacg ccccaaatcg gaggaatccc ccgaagcacc tcccgcttga
     3301 attcccgaga aagtgtggtc attgtttgtt cctcgatgcc gggcgggatg atttagaaaa
     3361 gcggagggcc tgagaaatgc gattaagcac aatgatcctt ggtatggccg ccctggcttg
     3421 cgtctcggtc gcctgcagca
//
LOCUS       DASRZF010000186        16230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4598023, whole genome shotgun sequence.
ACCESSION   DASRZF010000186 DASRZF010000000
VERSION     DASRZF010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 16230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16230
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4598023"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VFG22_08185"
     CDS             <1..189
                     /locus_tag="VFG22_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416263.1"
                     /translation="YHAGPKAARTAISEHEGTGLSQNILIRDKVVAVGLGAVPQAAPR
                     DEIAVERLSWVVRLGGHG"
     gene            182..1141
                     /locus_tag="VFG22_08190"
     CDS             182..1141
                     /locus_tag="VFG22_08190"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HET6416264.1"
                     /translation="MADTDFYAVLGVSRSSSPEEIKKAYRQLAKKLHPDRNPDNKAAE
                     ERFKDVSAAYMVLSDPEKRKLYDEFGEMGLREGFDPEVYHAASQGAGGFGGFDFGEIF
                     RGGGRGSGGGGRHVEFNLEDLFGGAGPGRASYVRAPQRGADLRSEVTIDFRDAVLGCT
                     KELSLRSAEGERTIKVRIPAGARNEGKIRLRGQGGSGAQGGPAGDLVLQVRVRKHPYF
                     SMRGKQLHVQVPVTPLEAYAGAKVSIPTPQGSVQLSVPAGSQNGSKLRLRGKGIQSKE
                     GPRGDLIAHLEIVLPKGKSKEVEEALQTVQDAFDGDPRSALEF"
     gene            1259..2422
                     /gene="bioF"
                     /locus_tag="VFG22_08195"
     CDS             1259..2422
                     /gene="bioF"
                     /locus_tag="VFG22_08195"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HET6416265.1"
                     /translation="MGFQRQIREALAELEAAGLLRSPVRISGPQGPEVEINGAKVLCF
                     CSNNYLGLASHPAIAAASAASPANDGVGAAASRLVTGTMDAHLDAEAAYADFVHAPAA
                     ALFSSGYAANVGAIQALAGPGDAIFSDALNHASVIDGARLSRAEVFVYSHRDPEHLQS
                     LLAQHRARARKALIVTDSLFSMDGVIAPLRELHALARDYDAGLMVDEAHALGVFGPNG
                     RGLSAALGIEPDVIVGTLGKSFGLAGAFVAASEEVVTLVRNRARSFVYSTAPPPMLAR
                     AAIAALRLVREADHARVRLLEHASKLRSGLAQSGFEIPEGESQIIPVLIGENRRTMQL
                     SAKLLERGVFVQGIRPPTVPAGTARLRLTPMATHQAEQIEQVIAAFASIVLKR"
     gene            2434..3108
                     /gene="bioD"
                     /locus_tag="VFG22_08200"
     CDS             2434..3108
                     /gene="bioD"
                     /locus_tag="VFG22_08200"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HET6416266.1"
                     /translation="MKGAFITATGTGVGKTWLATALTRACVQTGKRVASIKPIESGVD
                     PDPLDALELARACGNESLARAPGLYRAKEPLAPYAIEKRGGPAVGSLRKLAARIRELA
                     SDADYTIVEGVGGVLVPIDREHSIADLASLLDLQTVLVARNSLGVLSYTLTAVEALRK
                     RKISVHAIVLNDVEGDTENDLSRSSNADVLADYFPRIAILSVPRVQSPEQLVDAASQL
                     LKILKV"
     gene            complement(3152..3901)
                     /locus_tag="VFG22_08205"
     CDS             complement(3152..3901)
                     /locus_tag="VFG22_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416267.1"
                     /translation="MGETQGIARCASGAAVLSLALLFAMPALAQQTPDDAAAREYFER
                     GRSAFEEADYESSLVYFRHAYGLSGRSQLQYNIGVAADRLQREEEALDAFEQYLRDTE
                     NPAREAEVRKRVTALRKSIAERKATERALEEAAIRYQAAGRLEDTSDGKRIGRSALIG
                     GSALAAVGVGGIAAMAVGLARDGACSDQVAGRCVTESAATSWTWVYGGLGIAALAGSA
                     SWIAVSAKRRKSKGETEVSITPTGFMVSGKF"
     gene            4033..4105
                     /locus_tag="VFG22_08210"
     tRNA            4033..4105
                     /locus_tag="VFG22_08210"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4066..4068,aa:Ala,seq:cgc)
     gene            4181..5269
                     /gene="serC"
                     /locus_tag="VFG22_08215"
     CDS             4181..5269
                     /gene="serC"
                     /locus_tag="VFG22_08215"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HET6416268.1"
                     /translation="MARVINFNAGPATLPLPALEYAHNEFLDHEGTGMSLIEHSHRAA
                     AYEKVHQEAKSLFRELLDIPDTHEVLFMQGGASGQFALVPMNLRGEGKSADYINTGVW
                     SDKAMEEAKILGAAREAGSGKVDGKYTRVPNQAELDLDPKAAYVHFTTNNTIMGTQFH
                     TYPDTGNVPLVADMSSDILWRPIDVSKFGLIYAGAQKNLGPAGITILILRKDLAERSP
                     STIPKIFRYKTILAGDSLQNTIPTFPVYMVRNVLRWVRDQGGAPGMEKRNLEKAGYIY
                     GMIDENPDFFRCPVERESRSVMNPVFRLPSEDLEKKLIAEAAAAGMVGIKGHRSVGGI
                     RTSIYNAMEPSDVQTFVDFAKTFARKNG"
     gene            complement(5266..5982)
                     /locus_tag="VFG22_08220"
     CDS             complement(5266..5982)
                     /locus_tag="VFG22_08220"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HET6416269.1"
                     /translation="MNFERLLDVTTATAASAARPNFGNVHYARAKVPEQPLEIYEFEV
                     CPYCRIAREAMSALSLDPIVYPCPKGGALFREKVKRQGGRYQFPYLVDPNTGVSMYES
                     ADIVEYLFREYGDGRAPWFLKQRAFAVSTSMLASAFRPTRGRHAVRSRQPEKLLELYS
                     YEGSPFCRIAREALCELELPHRLHNVPRRSPDRDGFVAISGKMQVPYLLDPNTGIQMF
                     ESGDIRRYLYETYAVDPAEA"
     gene            6037..8073
                     /gene="uvrB"
                     /locus_tag="VFG22_08225"
     CDS             6037..8073
                     /gene="uvrB"
                     /locus_tag="VFG22_08225"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HET6416270.1"
                     /translation="MGRDLELITELRPKGDQPAAISELLQGLSNGDPHQVLLGITGSG
                     KTFTVANVIATTKRPTLIIAPNKTLAAQLYSEMRELFPNNAVEYFVSYYDYYQPEAYI
                     PSSDTYIEKDAIINDKVDRMRHSATRSLLSRRDVIIVASVSCIYGIGSSEWYQEMLIN
                     LEVGQEFRRDRLLRRLVDIQYKRNDIDFHRGTFRVRGDVVEIFPAHSENLAVRIEYWG
                     DEIERIVEIDPTRGKVHEELEQYGVYPGSHYVTPDEHRQRAIKSIRAELLEWLPKLES
                     EGKLLERQRLEQRTMYDLEMLEQMGFCHGIENYSRHLSGRKEGEPPPTLLDYFPDDFL
                     VILDESHITVPQLGAMFRGDRSRKETLVEHGFRLPSALDNRPLRFSEWQERVGQVIHV
                     SATPGDWEIEQAGGVLVEQVIRPTGLLDPQINVRPTKHQVDDLLGEIRQRVERNERVL
                     VTTLTKRMAEDLTEYYGELGVRIRYLHSDIDTLERVDILRDLRRGEFDVLVGINLLRE
                     GLDLPEVSLVGILDADKEGFLRSARSLIQTTGRAARNAGGEVIMYADRITDAMRYAIE
                     ETERRRERQARYNAEHGITPQTIKKAVLEMDPSTGQGDYVVIPILKRGELPGSPADIP
                     ARIEELRSEMLLAAEELEFEKAADLRDQVEQLRSQLHPGKSDRKRDRRSQRPKR"
     gene            complement(8102..9022)
                     /locus_tag="VFG22_08230"
     CDS             complement(8102..9022)
                     /locus_tag="VFG22_08230"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET6416271.1"
                     /translation="MPGIEVEFEVEEDDAGYRLDVFLARRASGLSRARAKRMIEEGGV
                     LVDGRRVRKSYVLAQGNRITLESLAPRVDFHARPDPDVPIVIVRETDRYVLVEKPAGV
                     PSHPLEEDELGTMAGALVARYPEMRGVGYSNREPGLLHRLDTHTSGLMLAARNREAFD
                     ALRQQLRSGEIEKRYLARCQGVVDAPIVIETAIANDPRDRRKVRTCTDPREIKRLGAQ
                     AARTEVLSSIPAAMGSLLELRANNARRHQIRVHLASIGHPLLGDTLYGGPALDEPAHH
                     LLHASAIKLGTETMVTSLWKYAISDPLPAS"
     gene            9102..10196
                     /gene="dnaJ"
                     /locus_tag="VFG22_08235"
     CDS             9102..10196
                     /gene="dnaJ"
                     /locus_tag="VFG22_08235"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HET6416272.1"
                     /translation="MQKRDYYVILEVSRAASGDEIKQAYRALAMKYHPDRNPDDPAAE
                     DCFKEASEAYAVLSDPDKRSQYDRHGHSAFEAGGPGFDGSDFGAVSEILEGLFGEVFS
                     GGRRKKRSARDLNYDLEISFVEAALGTDKTIDVSRPAPCESCDGTGAKPGTPVHKCNV
                     CQGRAQVKYQRGLFAASRPCHACGGSGKKIPTPCEACKGAGSQMRAESMSVKIPAGVQ
                     DGAVRTVRGAGEASASGNGDLHINIKVVDHALFQREGADILLSLPISFPQAVLGASIE
                     VPTLDGTVVMKVPPGTQSGKIFRLRGKGIPVYGGYGKGDQLVTVVVEVPQEISRRQRK
                     LITELAQEIGDEAHPQHASFLRKLRGLFEA"
     gene            10243..10920
                     /locus_tag="VFG22_08240"
     CDS             10243..10920
                     /locus_tag="VFG22_08240"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET6416273.1"
                     /translation="MRTAVMYMLAGALLVGCPKEEANQPTSEGEETPAPTPTKAVEVT
                     QKASQAPAQYTVELDTSKGAIVIDVRRDWAPHGADRFYELVQNGYYTDVAFFRVIGGF
                     MAQVGISGDPALNAQWRENRIPDDAVKASNTRGTVTFATSGPNSRTTQFFINFDDNSR
                     LDAMGFAPIGKVKDMGPVDALYSGYGEGAPNGRGPSQPLMQSQGNSYLRESFPKLDYI
                     KSAKIIE"
     gene            complement(10910..11980)
                     /locus_tag="VFG22_08245"
     CDS             complement(10910..11980)
                     /locus_tag="VFG22_08245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HET6416274.1"
                     /translation="MTLLRARGFRNLQALEFEPGSHFNVIHGDNGAGKSNLLEAIYYL
                     GALKSFRGAKTDDLIALESEAASLEARLESGPAPQKLRIDIARSRPRRLQLDGKRPRS
                     TSAWHQTVRMVLFHPGDLVLAAGAADKRRAFLDRMLEQMDPIYGSTLASYEKALRSRN
                     RLLKDERADRRSVRAYDAILSKAGAVVGKARARLIDDLAPRVVRAFSEVFAGDTPLGV
                     RYLPRVEPSEEMIAAALERAYEKDRARGFTADGPHGDDLELQLHEVGARHHGSQGQHR
                     TIVLALKTAELDLLTERTGRVPILLLDDVSSELDRGRNRRFFGVLSQAGGQVFLTTTH
                     PEFILLDQDRVDFHVEGGRVTR"
     gene            complement(12037..13173)
                     /gene="dnaN"
                     /locus_tag="VFG22_08250"
     CDS             complement(12037..13173)
                     /gene="dnaN"
                     /locus_tag="VFG22_08250"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HET6416275.1"
                     /translation="MDLTIDKQQFLRGLARTHGVADRKSSMHILSNVLLSAEGPDRLR
                     LSATDLYLGVTAVVPASIKKGGTIAVAARTLFDIVKNLPDGEVSWKLSDSHAVELSCG
                     KVRYRIPAMPGEDFPPLPNPGDADFAHLDAGVLAELISLTQYSMSHDDTRPHLAGTLF
                     EGDGKVVRMVTTDGHRLSKAEHKVGGKGSMMNFTMLVPHKGISELKKLLDDIKGGKGK
                     GDEHATIGIATAGGNAFFLRDDLCLSVKLADENFPPYSKVIPSKQSRTVIAARGPLVE
                     ALRRISLVANDKSGGVQLIIEPGMLRLQSQNPEVGEGSEEVDVDYAGDELRIGFNARY
                     LLDALTALPHDEVAIELSGERDPGVVKPVGGNSDFIGVIMPMRI"
     gene            complement(13524..14882)
                     /gene="dnaA"
                     /locus_tag="VFG22_08255"
     CDS             complement(13524..14882)
                     /gene="dnaA"
                     /locus_tag="VFG22_08255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HET6416276.1"
                     /translation="MEHLWEKALVSLKGDLSTENFETWLKPLQFGGVENDFIVLRIPN
                     QFFAEWIAAHYLDLILETLQSHAGDSPVPTKVRWELDETLREKAQAARESTPPPAPRA
                     PRATPQAPITADLNPKYRFKNFVVGPANQLAHAASVACGSDPGRRYNPLFIYGGVGLG
                     KTHLVNAIGHAIIQRNPRARILYVSAERFTNEFIWSLQNHEINSFRKRYRTDCEVLLM
                     DDIQFLAGREQTQEEFFHTFNALYHSDKQIVVTSDVYPQQISEMQERLISRFQSGMVA
                     DVQAPELDTRIAILQQKAESEGIELRGEVALFLAQTVRSNVRELEGTLLRIAVKAELL
                     GIPMDLELAKETMRAVIPNQDTEMTVEDIQRIACGYFGIRLADLKSRRRHRAVSFPRM
                     VAMYVCRQRLGTSYPELGERFGGKDHTTVISAVRKIGGLIESQDERVLGAVGAIERKL
                     GI"
     gene            complement(15331..>16230)
                     /locus_tag="VFG22_08260"
     CDS             complement(15331..>16230)
                     /locus_tag="VFG22_08260"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET6416277.1"
                     /translation="VPVWVALGLFIGLLRGRAFIILRLLIFAWFYFGFELIALLLVGG
                     VFLSRPAGEARSEALYRLQAWWGSLNLKAAQKLLRLQIEVEGARCVVPGPTILLIRHA
                     SILDTLLPATYVQRPYGFRIRYVLKQELLFDPCIDIVGNALPNYFVDRTGNTDRELEG
                     IRSLVAQLGTDGVLIFPEGTRFSQKKRSKALRMLSATRPELHDAAQAMTHVLPPKPGG
                     VLALLDALPETDCVFFAHSGLEAFAKIKDLMSGEVVGSTVRVHMWRVDAREIPPSEEE
                     RLVWLYREWAKVNALVGNQTETR"
BASE COUNT         3260 a         4995 c         4907 g         3068 t
ORIGIN      
        1 tatcatgcag gtccaaaggc agcaagaacg gctatctcag agcatgaggg gacaggcttg
       61 tcacaaaata tactcataag agacaaagtt gtcgcagttg gcctcggcgc agtccctcaa
      121 gccgctccgc gcgatgaaat cgccgttgaa aggttatcct gggtggttag gttaggtggt
      181 catggctgac actgatttct acgcagtctt gggggtttcg cggagctcga gccctgaaga
      241 gatcaagaag gcctaccggc aactcgccaa aaagctgcat cctgatcgga accctgacaa
      301 caaggcggca gaggaacgat tcaaagacgt gtcggcggcc tacatggtgc tgtccgatcc
      361 cgagaaacgg aagctctacg acgagtttgg tgagatgggt ctgcgcgaag gattcgaccc
      421 tgaggtttac cacgcggcaa gccaaggagc cggcggcttc gggggcttcg acttcgggga
      481 gatctttcgc ggaggggggc gcggcagtgg cggtggcggc cgtcacgtcg agttcaacct
      541 ggaggatctc ttcgggggag cgggaccagg tcgggcctcg tacgtgagag cgccgcaaag
      601 aggcgctgac cttcgctccg aggtgacgat cgatttccgc gacgccgttc tcggctgtac
      661 caaggagctg agccttcgct cggcggaggg ggagcgaacg atcaaagttc gaatcccggc
      721 cggcgcgcgc aatgagggca agattcgcct ccgcggacag ggcggctccg gagctcaggg
      781 cgggccggcg ggcgacctgg tcttgcaggt cagggtgcga aagcaccctt acttctcgat
      841 gcgtggcaag cagcttcatg ttcaggtgcc cgtgactccc ttggaggcct atgcgggtgc
      901 caaggtcagc attccgactc cgcaaggaag cgttcagctc tccgttccgg ctggctctca
      961 gaatggctct aagctgagac tccgcggaaa gggcatccag tcaaaggagg gtccccgcgg
     1021 cgatttgatc gctcacctcg agattgtgct cccgaagggg aagtccaaag aggtcgaaga
     1081 ggctctccag acggtgcaag acgcctttga cggcgatccc cgcagcgctc tcgagttctg
     1141 aatgtttcac gtgcaacacc gacctccgag ctcatccgca gcgcgagatg tttcacgtgc
     1201 aacattgccc catcggactc gtttgacgcc cagggacccc gcgccttaga ttccgcgcat
     1261 gggctttcag cgccagatcc gggaggccct agcggagctg gaggcagcgg gactcctacg
     1321 gagtcctgtt cgcatctcgg gtccacaggg ccccgaggtc gaaatcaatg gcgccaaggt
     1381 cctctgcttc tgctccaaca actacctcgg cttggcgagc catcctgcga tcgctgccgc
     1441 ctcagctgcc tcgcccgcca acgacggcgt cggtgcagcg gcatcccgtc tcgtcacggg
     1501 aaccatggac gcacacctcg acgcagaggc cgcctacgca gacttcgtcc acgctccggc
     1561 cgccgctctc ttctcgagcg gctacgctgc caacgtcggt gcgatccaag ctcttgccgg
     1621 tcctggggac gccatcttca gcgacgcgct caatcacgct agcgtgatcg acggggcgcg
     1681 cctgtcacgc gctgaggtgt tcgtctactc ccatcgcgac ccggaacatc tccaatctct
     1741 gctcgcgcag catcgcgcga gagcccggaa ggccttgatc gtcaccgact ccctcttctc
     1801 gatggacggc gttatcgcgc cgctccgaga gctccacgcc ttggctcgcg actatgacgc
     1861 cggtctcatg gtcgacgagg cccacgcact tggtgtcttc ggaccgaacg gacgcggcct
     1921 gtctgcagcg ctcgggatcg agcccgatgt catcgtcgga acacttggaa agtccttcgg
     1981 cctggccggg gcgttcgtcg cggcaagcga agaggtggtg acgctggttc gcaatcgcgc
     2041 gcggagcttc gtctattcga cggcgcctcc accgatgctc gcccgcgctg cgatcgccgc
     2101 gctccgtctg gttcgcgaag ccgaccacgc gagggtcagg ttgctggaac acgcctcgaa
     2161 gctgcgttcc gggcttgctc aatcggggtt cgaaatcccg gagggagaaa gtcagatcat
     2221 ccctgttttg attggggaaa atcgtcgtac gatgcagctc tcggcgaagc tgctcgaacg
     2281 aggggtcttc gttcagggca tcagaccacc gacggtgccc gcgggtacag ctagacttcg
     2341 gctcacaccc atggccacgc accaagcaga gcaaatcgaa caggtgatcg cggccttcgc
     2401 ttcgatcgtc ctcaaacgat aggaggccag cggatgaaag gcgcgttcat cacagcaacc
     2461 ggcaccggag ttgggaagac ttggctcgcg actgcgttga ccagagcctg cgttcaaacc
     2521 ggcaagcgcg tcgcttcgat caaaccgatc gaatccggag tggatccaga tccgctggac
     2581 gcgctcgagc tcgcccgtgc ctgcggcaac gagtccctcg cgagagctcc gggcctctac
     2641 cgagccaagg agccactcgc cccgtacgcg atcgagaagc gtggaggtcc ggccgtcgga
     2701 tcgcttcgca agctagccgc gcgcatccgg gagctcgcaa gcgatgcgga ctacactatc
     2761 gttgaagggg tcggcggggt tctggttccg atcgaccggg agcacagcat cgcggatctc
     2821 gcttcgcttt tggatctgca gacggtgctc gttgcgcgca actcgcttgg agtgctctcg
     2881 tacacgctca ccgcggtcga agcgctccgg aaaagaaaga tctcggtgca cgccatcgtg
     2941 ctcaacgacg tggagggcga taccgaaaac gacctgagtc gaagctccaa cgcggacgtc
     3001 ttggccgatt acttcccgcg catagcgatt ctgagcgtgc ctcgcgtcca aagtccggag
     3061 cagctcgtcg acgccgcttc gcagctgctc aagatattga aagtttgaag gttctcaagc
     3121 accccccgga agaatcactc gtgatactag cctagaactt tcctgatacc atgaagccgg
     3181 tcggagtgat ggaaacctcc gtttcgccct tgctcttcct ccgcttcgca ctgactgcga
     3241 tccagcttgc actgcctgcc aacgcggcga ttccaagtcc tccgtacacc caagtccagg
     3301 acgtcgccgc gctttcggtg acgcagcgcc ccgcgacttg atctgagcac gcgccgtcgc
     3361 gcgcaagtcc cacggccatg gccgctatgc cgccgacacc caccgcggcc aatgcacttc
     3421 ccccgatgag cgctgagcgc ccaatccgct tgccatcgct cgtgtcctcg agccgccccg
     3481 ctgcttggta acggatggcg gcttcctcga gcgcgcgctc ggtcgccttg cgttctgcga
     3541 tcgattttcg aagcgcagtg acgcgtttcc gcacctccgc ttcgcgcgcc ggattttcgg
     3601 tgtcgcgcag gtattgctcg aacgcgtcga gagcttcctc ttctcgctga agtcgatccg
     3661 ctgccacgcc gatgttgtac tgaagctggc ttctcccact caagccgtat gcatgtcgga
     3721 agtagaccaa cgaggattcg tagtcggcct cttcgaacgc tgagcgaccg cgctcgaagt
     3781 actctcgcgc cgccgcgtcg tccggcgtct gttgcgcgag agccggcatg gcgaacagca
     3841 gcgcgagcga caaaactgcc gccccgctcg cgcagcgagc tattccttgc gtctccccca
     3901 tggcttcaag gtagaggctt ggagacccca tcggcaagcg ggtccccggg ccagaaatta
     3961 cctcctgagt gcctgatcag gcccggtcga gttgcacgcg gaggcggcgc ggctatacga
     4021 acgcccgacc tcggggttgt agctcagttg ggagagcgcc gcgttcgcaa tgcggaggtc
     4081 aggggttcga tccccctcag ctccaccgtt tttcgaggtg ttccgcccgg acgaccgagc
     4141 gagttgcgcc gccccaaacc gcggctaggc tccgccgcct atggctcgcg tcatcaactt
     4201 caatgcggga cccgcaaccc ttccgttgcc ggcactcgag tacgctcaca acgagttcct
     4261 cgaccacgag ggcaccggga tgtcgctcat cgagcacagc catcgcgctg cggcgtacga
     4321 gaaagtgcac caagaagcga agtctctctt tcgcgagctc ctcgacattc ccgacaccca
     4381 cgaagtcctc ttcatgcagg gtggagcgtc aggtcagttc gctctcgtcc cgatgaatct
     4441 tcgcggcgaa ggcaagtctg ccgactacat caatacgggc gtctggagcg acaaagccat
     4501 ggaggaggca aagatcctcg gcgcggctcg cgaagctggg agcgggaagg tcgacgggaa
     4561 gtacacgcgc gtcccgaatc aggccgagct cgacctggat ccgaaggcgg cctacgtgca
     4621 cttcacgacg aacaacacga tcatgggcac gcagttccac acgtaccctg acactgggaa
     4681 cgttcctctc gtcgcggaca tgtcgagcga catcctctgg cgaccgatcg acgtttccaa
     4741 gttcggtctg atctacgcgg gagcccagaa gaacctcggc ccagcgggga tcacgatcct
     4801 gattcttcgc aaggacttgg cagagcgcag cccgagcacc atcccgaaga tcttccgata
     4861 caagaccatc ctcgcaggcg actcacttca aaatacgatt ccgacgttcc cggtctacat
     4921 ggtgcgcaac gtcctgcgtt gggtccgtga tcagggtggc gccccgggta tggagaagcg
     4981 gaacctcgag aaggccggct acatctacgg catgatcgac gagaaccccg acttcttccg
     5041 atgtccggtg gagcgcgagt cccgatcggt gatgaatcca gtcttccggc ttcctagcga
     5101 ggacctcgaa aagaagctca tcgccgaagc ggccgctgcc ggcatggtcg ggatcaaagg
     5161 ccaccgttcc gtcggcggca tccgcacctc gatctacaac gccatggagc cgtccgacgt
     5221 tcaaacgttc gtggacttcg ccaagacgtt cgctcgcaaa aacggctagg cttcggccgg
     5281 gtcgaccgcg taggtctcgt agagataccg gcgaatgtcg cccgactcga acatctgaat
     5341 tccggtgttt ggatcgagca aatagggcac ttgcatcttt ccggaaatcg ccacgaagcc
     5401 gtcccgatcg gggcttcggc ggggaacgtt gtggagccga tgcggaagct ccagctcgca
     5461 gagggcctcg cgggcgattc ggcagaaggg cgagccctcg tacgagtaga gctcgagcag
     5521 cttctcgggc tgccgcgagc gaaccgcatg cctgccccgc gtcgggcgaa acgcagatgc
     5581 gagcatcgac gtcgagaccg cgaacgcgcg ctgcttgaga aaccaggggg cacgtccgtc
     5641 cccgtactca cgaaagagat attcgacgat gtcggctgac tcgtacatcg aaactccggt
     5701 gttcggatcc acgaggtacg gaaactggta gcgacctccc tggcgcttga ccttctctcg
     5761 gaagagagct ccgcctttcg gacaggggta gacgatgggg tcgaggctga gcgccgacat
     5821 cgcttcccgc gcgatgcgac agtacgggca gacttcgaac tcgtaaatct cgagcggctg
     5881 ctctggcact ttcgcccgcg cgtagtgcac gttgccgaag ttgggccggg ccgcactggc
     5941 cgccgttgcg gtggtcacgt cgaggagccg ttcgaagttc atacgctgct cactaatggg
     6001 cctgctcccg ttttcaaggg gtgataggct ccgctgatgg ggagggacct tgagctcatc
     6061 accgaattgc ggcccaaggg agaccagcca gcggcaattt cggagctttt acagggactt
     6121 tcgaatgggg acccgcatca ggttctgctc ggaatcacgg ggagcggaaa gacgttcacg
     6181 gtcgccaacg tcatcgcgac caccaagcgc ccgacgctga tcatcgcgcc caacaagacc
     6241 ttggccgccc agctctacag cgagatgcgc gagctctttc cgaacaatgc ggtcgagtac
     6301 ttcgtcagtt actacgacta ctaccagccc gaagcctaca tcccctcgag cgatacatac
     6361 atcgaaaaag acgcgatcat caacgacaag gtcgaccgca tgcggcacag cgcgactcgc
     6421 tccctgctgt cgcggcgcga cgtgatcatc gttgcgagcg tctcctgcat ctacggtatc
     6481 ggttcatccg aatggtacca agagatgctg atcaacctgg aagtgggtca ggagttccga
     6541 cgcgaccgct tgctccggcg gctcgtcgac attcaataca agcgcaacga catcgatttc
     6601 catcggggga cgttccgggt gcgcggtgac gtcgtggaga tcttcccggc gcactcggaa
     6661 aacctggccg ttcgtatcga gtattggggc gacgagatcg aacgaatcgt cgagatcgac
     6721 ccgacacgag gcaaagtgca cgaggagctg gagcagtacg gcgtctatcc ggggagccac
     6781 tacgtcactc cggacgagca ccgccaacgg gcgatcaaat cgatccgcgc cgagctcttg
     6841 gaatggctcc caaagctcga atctgagggc aaattgctcg aaagacagcg tctcgagcag
     6901 cgaacgatgt acgacctgga gatgctcgaa cagatgggat tctgccatgg aatcgagaac
     6961 tacagccggc acctttcggg gcgcaaagaa ggagagccgc cgccgacctt gctcgactac
     7021 ttcccggacg attttctggt gattctcgat gaatcacaca tcacggtgcc tcagctcggt
     7081 gcaatgtttc gcggtgatcg ttcgcggaag gagaccttgg tggagcacgg gtttcgattg
     7141 cccagtgccc tcgacaaccg gcccctgcgc ttcagcgaat ggcaggaacg cgtaggccaa
     7201 gtgatccacg tgagcgcgac gccgggcgat tgggagatcg agcaagcggg cggtgtgctc
     7261 gtcgaacagg tcattcgacc tacgggcctc ctcgatccgc agatcaacgt gcgaccgacc
     7321 aagcaccagg ttgacgatct tctcggtgag attcgccagc gcgtggaacg aaacgagcgc
     7381 gtcttggtca cgaccttgac caaacgcatg gccgaagatc tcaccgagta ctacggagag
     7441 ctcggggtgc gcattcggta cctccacagc gacatcgaca ccctggaacg tgttgatatt
     7501 cttagagatt tgaggcgggg agagttcgac gttctggtcg ggatcaatct cttgcgagag
     7561 gggctcgatc tgcccgaagt ctcgctcgtg ggcattctcg acgccgacaa ggaaggcttt
     7621 ctccgttcgg ctcgttcgct gatccaaacg accggccgcg ccgctcggaa cgcaggcggt
     7681 gaggtcatca tgtacgcgga ccgaatcacc gacgcgatgc gatacgcgat cgaagagact
     7741 gagagaagac gcgagcgtca agccaggtac aacgcggaac acggaatcac tccgcagacg
     7801 atcaagaagg ccgtcctcga gatggatcca tccacgggac aaggcgacta cgtcgtgatt
     7861 ccgattctga aacggggtga gttgccagga agtcctgctg acatacccgc gcgcatcgaa
     7921 gagctgcgaa gcgagatgct actcgccgcc gaagagctgg agttcgaaaa ggccgcagac
     7981 ctccgtgatc aggtcgagca gcttcgttcc cagctacatc ctgggaagag cgatcgtaaa
     8041 cgtgatcgac gatcgcagcg cccaaagcgt tagcccctca aaaatgccca ttttgcggct
     8101 ctcacgatgc tggcaagggg tctgagatcg catacttcca aaggctcgtg accatggtct
     8161 ccgtccccag tttgatcgcg ctggcgtgaa ggagatggtg cgcgggctcg tcgagcgccg
     8221 ggcctccata gagcgtgtcg ccgagcagag ggtgacctat cgaagccagg tggacgcgga
     8281 tttgatgtcg ccgggcgttg ttggcacgga gctcgagcaa ggagcccatg gcggcaggga
     8341 tgctgctgag gacctcggtc cgggcggcct gggcgccgag gcgcttgatc tcgcgcgggt
     8401 cggtgcaagt gcgaaccttg cgacgatcgc gagggtcgtt cgcaatcgcc gtctcgatga
     8461 ctatgggtgc gtcgacgacg ccctggcatc gcgcgaggta tcgcttctcg atctctcccg
     8521 aacgaagttg ctggcggagc gcatcgaagg cttctcgatt gcgagccgcc agcatcagtc
     8581 ctgaagtgtg ggtatcgagg cgatggagca gcccgggctc tcgattggag tatcccacgc
     8641 cgcgcatctc gggataccgt gcgacgagcg cacccgccat ggtgccgagc tcgtcctctt
     8701 cgagtggatg acttggaact cccgccggct tctcgaccag gacgtagcgg tcggtttcgc
     8761 gcacgatcac gataggcacg tctgggtctg gccgggcatg aaagtcgact cggggcgcaa
     8821 ggctttccaa agtgattcgg ttgccctggg cgaggacgta gctctttctg acccttcgcc
     8881 cgtcgacgag aacgcctcct tcctcgatca tgcgtttggc cctggcccgg ctcagcccgg
     8941 acgcacggcg agcgagaaaa acatccagcc ggtacccggc gtcatcctct tcgacctcga
     9001 actcaacctc gatgcctggc atttgagata catcccacat ccggccgcac cgctccatgc
     9061 catttgcacc gggtgtgggc gcgtctacac tccgcaaacc tgtgcagaaa cgtgattact
     9121 acgtgatcct cgaagtcagc cgcgccgcgt cgggcgacga gatcaagcaa gcctatcgcg
     9181 cgctcgcgat gaagtaccac cccgaccgaa atccggacga cccggctgcc gaggattgct
     9241 tcaaagaagc gagcgaagcg tacgcggtct tgagtgatcc cgacaaacgc agccagtacg
     9301 atcgccacgg tcactctgcg ttcgaagccg gcggcccagg cttcgatggg agtgacttcg
     9361 gtgcggtgag cgagattctc gagggcctct ttggggaggt cttcagtggt gggcgtcgca
     9421 agaagcgcag cgctcgcgat ctcaactacg acctagaaat ctctttcgtc gaggcggcgc
     9481 tcggcaccga caagacgatc gacgtcagcc gacccgcgcc atgcgagagc tgtgacggaa
     9541 ccggtgcgaa gccaggtacc ccggttcaca agtgcaacgt ctgccaggga cgtgcgcagg
     9601 tgaagtacca gcgcggactg ttcgcggcct cacgtccctg ccacgcatgc ggcggctctg
     9661 ggaagaagat cccaacaccc tgtgaagcat gcaaaggcgc tggcagtcaa atgcgggcgg
     9721 aatcgatgag cgtgaaaatc cctgccggcg ttcaggatgg cgcggtgcgc accgtccgag
     9781 gggcagggga agcgagcgca tccggaaacg gcgatcttca catcaacatc aaggtcgtcg
     9841 accacgccct cttccagcga gaaggggcgg acatcctgct ttcgctcccg atctcgtttc
     9901 cacaggcggt gctcggagcc agcattgagg ttcccaccct cgatggcacg gtggtcatga
     9961 aagtcccacc cggaacgcag tcgggcaaga ttttcagact ccggggaaag ggaattccgg
    10021 tgtacggggg ctacgggaaa ggcgatcagc tggtcactgt cgtcgtcgag gttccccaag
    10081 agataagccg tcggcagcgc aagctgatca ccgagcttgc ccaggagatc ggcgacgagg
    10141 ctcaccccca gcacgcgagc ttcctgagaa agcttcgcgg gctattcgag gcgtagcggg
    10201 ccaagagctt gcgctcgcgg cggggcttgc caaggtacgg ccatgcgaac cgcagtcatg
    10261 tacatgttgg ccggagcact cctcgtcggg tgccccaaag aagaagcaaa tcagcccacg
    10321 agtgaggggg aagagacccc tgcaccgact cccacgaagg ccgtcgaggt gacgcaaaag
    10381 gcatcacaag ctcccgccca atacacggtc gagctcgaca cgagcaaggg cgccatcgtg
    10441 atcgacgttc gtagagattg ggcgccccac ggtgcggacc gattctacga gctcgtgcag
    10501 aacggctact acaccgatgt ggcgttcttt cgagtgatcg gcggcttcat ggcccaggtc
    10561 ggcatcagtg gcgaccctgc gctcaacgcg caatggcgtg agaaccggat ccccgacgac
    10621 gcggtcaagg cgagcaatac gcgcggcacc gtcaccttcg cgacctcagg accgaactcg
    10681 cgtacgaccc agttcttcat caacttcgat gacaattccc gactggacgc catgggcttc
    10741 gcgccaatcg gcaaagtcaa agacatgggg ccggtggacg cgctttacag cggctatggc
    10801 gaaggtgccc cgaacggccg aggcccgtcg caacctttga tgcaatccca gggtaactcc
    10861 taccttcggg aaagctttcc gaagctcgac tacatcaaga gcgccaagat catcgagtga
    10921 cgcggccgcc ctcgacgtga aagtcgactc gatcttggtc gagcagaatg aactccgggt
    10981 gcgtcgtggt gaggaagact tggcctcccg cctgactcaa gaccccgaaa aaacggcgat
    11041 tccgccctct gtcgagctcg ctcgaaacgt cgtctaacaa gagaattggt acacggccgg
    11101 tccgctctgt gagcaaatcc agctccgccg tcttgagtgc caggacgatt gtgcggtgct
    11161 gtccctgcga gccgtggtga cgcgcgccga cttcatggag ctgaagctcg agatcatcgc
    11221 catgtggtcc gtccgcagtg aaccccctgg ctcggtcttt ctcgtatgcg cgctccagtg
    11281 ccgcggcgat catttcctcg cttggctcca cccgaggcaa atagcggacc cctagtggtg
    11341 tgtcgcctgc aaacacctcc gagaaggcgc ggaccactct tggggcgaga tcatcgatca
    11401 gacgcgcacg agccttccct acgaccgccc cggccttgct cagaatcgcg tcataggcgc
    11461 gaaccgaccg tctgtccgcg cgttcgtcct tgagcaatcg attgcggctg cgaagcgcct
    11521 tctcgtaact agcaagcgta gaaccgtaga ttggatccat ctgctcgagc attcgatcga
    11581 gaaacgcgcg acgcttgtcc gccgcacccg ctgcgagcac caagtctccc gggtgaaata
    11641 gaaccattcg tacggtctga tgccaggcgc tcgtcgagcg cggacgcttt ccatcgagtt
    11701 gaagacggcg gggccgcgaa cgcgcgatgt cgatccgtag cttctgcggc gccggcccgg
    11761 actccagcct tgcttcgaga ctggcggcct cggattccaa cgcgatcagg tcatcggtct
    11821 tggcgccgcg aaagctcttg agcgcgccca gatagtagat cgcctcgagg agattcgact
    11881 tcccggcccc gttgtcgcca tgaatcacgt tgaaatgcga gcccggctcg aattccaggg
    11941 cctggaggtt tcgaaagccc cgagcgcgca gcagggtcac ccggcttgga ttgagagcgc
    12001 tccgcgaatc cgaggtcaac gccctgcccc gctcgactag atgcgcatgg gcatgatgac
    12061 gccgatgaag tcggagttgc cgccaacagg cttcactacg cccgggtcac gctcaccgct
    12121 gagctcgatc gccacctcat catgaggcaa ggcggtcaaa gcatcgagca aataccgggc
    12181 gttgaacccg atccgaagct cgtcgcccgc atagtccacg tcgacctctt cgctaccctc
    12241 accgacttcg gggttctggc tctgcagtct caacatgcct ggctcgatga tcagctgtac
    12301 accgccgctc ttgtcgttcg ccacgaggct gatgcgccgc aacgcctcga cgagcggccc
    12361 gcgggctgcg atcaccgtac ggctctgctt gctcggaatg accttgctgt aaggcggaaa
    12421 attctcgtcc gcgagcttca cgctgaggca caggtcgtca cgtaagaaga acgcgtttcc
    12481 cccggccgta gcgatgccta tggtggcgtg ctcgtcgccc ttgcccttac cccccttgat
    12541 gtcgtccaag agcttcttga gctcgctgat gcccttgtgg ggcaccagca tggtgaagtt
    12601 catcatcgag cctttgccgc caaccttgtg ctcggccttc gagaggcgat gaccatcggt
    12661 cgtcaccatg cggacgactt ttccatcgcc ctcgaacagg gtccctgcga gatgcggccg
    12721 ggtatcatcg tgagacatgg agtactgcgt gagcgaaatt agctcagcga gaacgcctgc
    12781 atcgagatgt gcaaagtctg catcacccgg atttggcaag ggaggaaaat cctcaccggg
    12841 catggcaggg atgcgatatc gaactttgcc gcaactgagc tcgaccgcat ggctgtcgct
    12901 gagcttccag ctgacctcgc catcggggag attcttgacg atatcaaaaa gcgtgcgggc
    12961 agcgacggct atcgtgccgc ccttcttgat gcttgcaggg acgacagcgg ttacgccgag
    13021 gtacaagtcc gttgcagata ggcgaagacg atccggacct tcagcgctaa gcaacacatt
    13081 ggacaagatg tgcatcgagc ttttgcgatc cgccacaccg tgggtgcgag cgagaccacg
    13141 taggaactgt tgcttatcga tcgtgagatc catcttttgg ttcaacccct gaagaactgt
    13201 gtctgtttat ccaccgcgtt agatctctta atttaaaaat tcgtcgtcgt cttaacagct
    13261 gtgaattgtg aatgaagctg ggtaactgag cgaaagaact aagattaaga tgaattcccg
    13321 tgaggggctt gatcctgagg cgattgggga caagccgact caccgttgat ctccagtggc
    13381 tttccccagg tagatctcag cctcggcaca ggcgctggta tacaccaaaa gcgcgtggaa
    13441 ggcccgaccc aggtagagcg cgcgaaccaa gccaattcgg ggaaaatccc gcctctgaat
    13501 cgaaacctcg aaacagccga cacctagatg ccgagcttac gctcgatcgc acccacggcg
    13561 ccgaggaccc gctcgtcctg ggactcgatc aggccgccga tcttgcgaac cgcgctgatg
    13621 acagtcgtgt ggtccttgcc gccgaagcgc tcgccgagct cgggatagct ggttccgagg
    13681 cgctgtcggc agacgtacat cgcgaccatt cgtgggaagc tgacggcgcg gtgacgtctc
    13741 cgcgatttca gatcagcgag gcggatgccg aagtagccgc aggcgattcg ctggatgtcc
    13801 tcgacggtca tctcagtgtc ctggttcggt atgaccgctc gcatcgtctc cttggcgagc
    13861 tcgaggtcca ttggaatccc gagaagctct gccttgacgg cgatccggag aagggtgccc
    13921 tcgagctcac gcacgttgct ccgtacggtc tgagccagga agagcgcaac ctcacctcgc
    13981 agctcgatgc cctcgctctc agccttctgc tgcaagatgg cgatgcgggt atcgagctcc
    14041 ggagcttgca cgtcggccac catgccagat tggaaacggc tgatcagacg ttcctgcatt
    14101 tcagagatct gctgcggata gacatccgag gtcacgacga tctgcttgtc cgagtgatag
    14161 agcgcgttga aagtatgaaa gaactcttcc tgtgtttgct cgcgacccgc caggaactga
    14221 atgtcgtcca tcaagagcac ttcacagtcg gttcgatatc gctttcggaa cgaattgatc
    14281 tcgtggttct gcaaagacca gatgaactca ttcgtgaaac gctctgcgga gacgtagaga
    14341 atgcgcgcac ggggatttcg ctgaatgatc gcgtgcccga tcgcattgac gaggtgtgtc
    14401 ttgccgaggc cgacgcctcc atagatgaag agggggttgt agcggcgccc cggatcggag
    14461 ccgcaggcca cacttgcggc atgcgcgagc tgatttgcgg ggccgactac gaaattcttg
    14521 aaacggtact tcgggttgag gtcagcagtg ataggcgcct gcggcgtagc cctgggtgcc
    14581 cgcggcgctg gcggcggcgt ggattcgcga gccgcttgtg ccttctcgcg cagcgtttcg
    14641 tcgagctccc agcgcacctt ggtcggcacc ggcgaatcgc ctgcatggct ttgcagcgtc
    14701 tcgagtatca ggtcgaggta gtgggccgcg atccactccg cgaagaactg attcgggatt
    14761 cgaagaacga tgaagtcgtt ctccacgccg ccaaactgaa ggggcttcag ccaggtctcg
    14821 aagttctccg tcgagaggtc cccctttagg ctaaccagcg ctttttccca cagatgttcc
    14881 atcgtctcct tacccccgaa aagcgcgcag ttatgcgtcc acgtccccag ggaattcaca
    14941 gcccctcggg aacgagcact cgtgtgccgt ccaccaacat ccgccggcct tggcgaccgc
    15001 cgacgtgtgt ccttccagtg aagggcgccg aaactagcag cgagtttcgg aggcgtctac
    15061 ggggatcgac gatcaaattt ctaaccgctt gtgatcgcga aagattcgca tgcagttttt
    15121 ttgacatcgg cgcgcgcaga tttacgcgtt gtcgccctct tgttgacaag ctgttgcaat
    15181 cattctgaat taagtcttgt tatcgtttgc gcaacataga ccgcggtttc atgctctgtt
    15241 cttccacagt tgtgtgtact tctcgtcgtg cacgatcacg aatgaatctg aagcgctcga
    15301 cactcgttcc acaacttgtc caccgacaag tcacctcgtt tccgtttggt ttccgacgag
    15361 cgcgttcacc tttgcccact cacgatacag ccaaacgagg cgttcctcct cgctgggtgg
    15421 aatctctcgc gcgtcgacac gccacatgtg aacgcgcacc gtcgaaccga cgacctcacc
    15481 gctcatgagg tccttgatct tcgcgaaggc ttcgagcccg ctgtgagcga agaagacgca
    15541 atcggtctcg ggcagcgcgt cgagaagggc cagaactcca cccggtttcg gcgggagcac
    15601 atgcgtcatc gcctgcgcag cgtcgtgcag ctccggcctc gtggcactga gcatacgtag
    15661 cgctttcgac cgttttttct gggaaaaacg cgttccctcg ggaaagatca gaaccccgtc
    15721 cgtcccgagc tgcgcgacca atgagcgaat cccttcgagc tcgcggtccg tgttgcccgt
    15781 ccggtcgacg aaatagttcg gaagggcatt cccgacgatg tcgatgcaag gatcgaatag
    15841 aagctcctgc ttgaggacat agcgaattct gaaaccgtac ggccgctgga cgtaggttgc
    15901 gggaagcaga gtatcgagaa tgctcgcatg gcggatcagc aggatcgttg gtccgggcac
    15961 cacgcatcga gccccttcga cctcgatctg caatcgaagt agcttttgcg ccgccttcag
    16021 attgaggctc ccccaccagg cttgaagtcg atagagcgct tcggaacgcg cttcgccggc
    16081 aggacgggac agaaagacgc cgccgacgag caatagcgca atcagctcga agccgaaata
    16141 gaaccaggcg aagatcagca gtcgaagaat gatgaaggca cgccctcgga gcaggccgat
    16201 gaacaagcca agtgcaaccc agaccggcac
//
LOCUS       DASRZF010000187         9592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2881067, whole genome shotgun sequence.
ACCESSION   DASRZF010000187 DASRZF010000000
VERSION     DASRZF010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 9592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9592
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2881067"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="VFG22_08265"
     CDS             complement(<1..774)
                     /locus_tag="VFG22_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416278.1"
                     /translation="MTAGRNRTVFVISLAFQGAAVIALTVAARSGAAPSLVVLCLAVC
                     FVPYAALLACAGALADPRAVSRIAIIAATLFGAVWVLAPPMLSDDLYRYLWEGRLWLE
                     GLNPYRFAPDDPVAAHLRDEAWSRINNRPLASIYPPLSQMLFVVAAAFGGTATSVKIL
                     ALLAHLVSAAVLGHVTSSSRATVALALNPLLLSEAALNGHLDILCGMALLIAAWALGQ
                     RRFVRAGAAALAAIGLKLVGLVLLPLLWRRPKVLLATALG"
     gene            complement(771..1412)
                     /locus_tag="VFG22_08270"
     CDS             complement(771..1412)
                     /locus_tag="VFG22_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416279.1"
                     /translation="MRKRLAIAASALLLACSGTGAGGPSSETAREVERAEEQPALVEA
                     PDLVSIVAIATAREIPRDCANPSEESCNGLDDDCDGVIDDGCGYGSGLMQVTASWDTG
                     ADIDLYVTGPLGDTLSFQRPSTPFGARVDHTGRGDCSGDTPNPRVENVRWVGQRPMNG
                     VYEVEAHYWGECGSAGGPTNVTISVAVGRRIAGQYLQSLLPGERIRVLRFVIQ"
     gene            1458..2264
                     /locus_tag="VFG22_08275"
     CDS             1458..2264
                     /locus_tag="VFG22_08275"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HET6416280.1"
                     /translation="MEALEQAKQAIAQRTGRPEVAMVLGSGLGDYADSLGGARAIPYG
                     EVPHMPLSGVSGHAGNFVVGEKAGKPIIVMQGRTHLYEGHSARDVVFGVRLMALLGAK
                     TLIVTNAAGGISPELQAGDLMAITDQLNLTGRNCLTGPNDQRRGPRFLDMTRAFDPAL
                     IESASSVSSRLGFTLKRGVYAGLLGPSYETPAEVRMLATLGADAVGMSTVLEVLAARH
                     MGLRVLGVSCISNLAAGIAEGPLSHEEVTETARRVRSRFEALLSGVLESL"
     gene            2264..2662
                     /locus_tag="VFG22_08280"
     CDS             2264..2662
                     /locus_tag="VFG22_08280"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET6416281.1"
                     /translation="MDEKAWDALKRSAMEARGRAYAPYSGFSVGAAIRTESGEVFIGC
                     NVENASYGATICAERAALAAAVAAGERELVALAIASDAHAPIPPCGICRQCLAELGTN
                     LPIRSYAGDAELEYQLSALLPDPFGREQLD"
     gene            complement(2670..6260)
                     /locus_tag="VFG22_08285"
     CDS             complement(2670..6260)
                     /locus_tag="VFG22_08285"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6416282.1"
                     /translation="MIKVNCPSCDASYDVDEHRLPDKGLRMRCPKCSDSFQVHRDGST
                     ARSGGGAAPGAKKPPRRKPTQVGLGPSSPPAGPPPPPVAVIKPQPSNLSANVEEIDLP
                     TPAGVRELADLPAPFAGRQQGDLPAPKKGGRSLDFDPFEVPGLADLPAPKTDPRASEF
                     DPFADMDLPAPLDSPGGTDLPAPMSHSYGGEIDLPVAKGATRARGIEQVGDEVDLPMA
                     LSDAELPTPRRGSAVPSPISLDSELPMAIGEEDLPVARDDFMDLDLEGPERVHGGGPI
                     ELDLPDGGDLDLDMDLEEPAKAPGPPPPLGHAFGAPGAAPEVARHADKRISDDADELE
                     FSELPGERADGVHRMPRPAGTGPEQVAPQKGKLKISVRRPPWLMKAVAALGVMALVLG
                     AGVYSGTTQYGLFGIHLLEPFLPAAGDELVVAQSIEEAEMIFAADTYAASQQALAHLQ
                     AARRDASLNRSLVARALLHESYHQVRYGQDATSAEVADSLRLHLQRRGDTATRVHVAL
                     AADALRNADNATAKSELALAAQDDPNDTYVDLVAGELWLRERDGQAARAAFERALQKH
                     ESARAQWGLARSSEILGDTGAAVAAARATLRLSPNHAAARVAVADSLIDEGKADEAYA
                     LLQIPAGLAEEQGVALQVARSDQSAALTRIARIEESRGRLGAAREMYDKAIELDTSNA
                     NATLGAARLVLLEGAYQDAYARFQTVLGSDIRPGAVMHPTGKPKVLVEAKLGAAEALL
                     AMNKAEEAKKLLADLETDEPVSAEVELLQGKVAENVGDSKAAVTHFRRAIVLAPKSFS
                     PYMALAQHYSSTKRPGEAVAVLLEAQKNVEITPEVRRVIGWAELQRNRVDDAVKEFLA
                     ALEMDPGDSASRFGLAVAYRRKGMLEEAAAELDEIQELDAKFPGLPLERGRLAEAGGD
                     MDAAASRYREALQESPKDAALKSRLGAVLVLTGQLDEGEKLLREVLTAQPYSAEAEHY
                     LGRIELERGQLESARQYFLRASRLETDKGLYRMYVAWVALEANEMTSALRELDATLKL
                     DPTLGDAYWLRARIRIRAGTVRDALADLNKAIELNPSRIEAWAAMGECHYQLGQMGEA
                     ISDWEKAVAGAPEHGYWWYRLGRLQLDEGRREEALTSLTTATSLGDPDPEDKGWLADA
                     HRLTGDIYFAQKKRQDAVVQYGRYLELADREAIDRADVEAKLRRIGQGLD"
     gene            6417..6734
                     /locus_tag="VFG22_08290"
     CDS             6417..6734
                     /locus_tag="VFG22_08290"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HET6416283.1"
                     /translation="MKRNVQIEIAGARYRMSSDADEAYLQRLADIVNERVNALGPKAA
                     RAASPAQLLAVVALSLAEDLDASERRRGDLETKTRELVSNAIRRIDNRLQADAELAQQ
                     IEP"
     gene            6731..7333
                     /locus_tag="VFG22_08295"
     CDS             6731..7333
                     /locus_tag="VFG22_08295"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HET6416284.1"
                     /translation="MSKEKTERLESQLRQRAKMALRSRMRAVRGALPDSACDARSKKI
                     DARLWELTELERASTVLAFGSIGNEVRTRAFMESAWAKGKRVGLPRVVGDELLLHLVE
                     RGTALAKGSYSVPEPAEDAPRLEPIDVDFALIPALAVDSRGYRIGYGAGYYDRLIPLL
                     KRACTCAVAYDFQLISEVPELPFDVAVDLVVTDERVIRSR"
     gene            7377..9275
                     /gene="uvrC"
                     /locus_tag="VFG22_08300"
     CDS             7377..9275
                     /gene="uvrC"
                     /locus_tag="VFG22_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HET6416285.1"
                     /translation="MVAEAAQRKLDKLPPSPGVYVFHGADGKVLYVGKARSLRSRVRS
                     YFQPGSSDLRAFVSRLERELVDIDTYVTHTDKEAALLENQLIKSHQPKYNVKLRDDKE
                     YLSVRLDTKKPWPRLEVVRRPKPDGAQYFGPYHSATAARQTLRLVNRYFQLRTCTDTE
                     LRLRSRPCLQYQIKRCPGPCVLDVDETEYRAQVTNVARFLEGRHGELVRDLDDRMKVA
                     SDELDYERAAIYRDQLRAVERAREEQRVAGVQKSDQDVIGFHREGDQVEVAVLRLRGG
                     RLFGVRTLPLRRVAVPNDEMLGAFLRQHYADRPDLPDEILVPTSIEMSEALEELLSEG
                     RNRRVRIVEPKRGAKAKLLTLAHENAEHAYGEKERAKEDVESRLADLQKQLRLSVLPR
                     RIECVDISHSGGEETVAVFVTLQDGTPARERYRSFRVKGVGGGDDYGAMYEVLMRRLR
                     RGRNEEEGWELPDLLVVDGGKGQLGVAVRAREDVGVPELEVASVAKPRVTVMGEEEGD
                     RVFRPGQKNAIAVRANSALSLLLLARDETHRASNVLRKKVGRKRRLRSELDGVPGVGP
                     KTRGKLLRNLGSLREVMAATEEELIEAGATRKQAEAIQRALGTQTSPPPETEAAEDTA
                     IDNAFQPD"
BASE COUNT         1813 a         3049 c         3096 g         1634 t
ORIGIN      
        1 gccaagcgct gtcgcaagca gcaccttcgg ccgtctccac aagagcggga gcaagaccag
       61 gccgacgagc ttcaggccaa tcgcagccaa ggccgctgca cccgcacgca cgaaacgtcg
      121 ttgcccgagc gcccaggccg cgatgagcag ggccataccg caaagtatgt ccaagtgacc
      181 gttgagagcg gcttcactca agagcaatgg attgagcgcc agtgcaacgg tcgctcttga
      241 cgaggaagtg acgtggccca ggacagcagc cgaaaccaga tgcgccagca acgccaggat
      301 cttcacggat gtcgcggttc cgccgaatgc tgcagccacc acgaacagca tctgcgaaag
      361 aggcggatag atgctggcga gcggtctgtt gttgatccgc gaccaagctt catcgcgtag
      421 gtgcgccgca accggatcat cgggggcaaa gcgataaggg ttcaaccctt cgagccagag
      481 ccgcccctcc cagagatagc gatagaggtc gtccgagagc atgggcggcg caagaaccca
      541 cacggcgccg aacagcgtcg cggcgatgat cgcaatgcgc gaaacggcgc gcggatccgc
      601 cagtgcgcct gcgcaggcga gcagagcggc gtaaggaacg aagcagaccg ccagacaaag
      661 cacgacgaga gaaggcgcgg cgcccgaccg cgccgcgacc gtgagagcga tgaccgcagc
      721 tccttgaaac gccaggctga tgacgaagac cgtgcggttc cggcctgccg tcattggatg
      781 acgaaccgca agacacgaat ccgctcaccc ggcagaagac tctgtaggta ctgacctgcg
      841 attcgacgcc cgacggctac tgagatcgtc acgttggtag gcccgccagc gctcccacac
      901 tcgccccaat agtgcgcttc tacctcgtag acgccgttca tgggtctttg accgacccaa
      961 cgaacgttct cgacgcgagg gttcggcgtg tccccggaac agtcgccacg tccggtgtga
     1021 tcgaccctcg cgccgaaggg cgtggagggc ctctgaaagc tcaaggtgtc gccgaggggt
     1081 ccggtgacgt agaggtcgat gtcagcaccc gtatcccaag aagccgtgac ctgcatcaga
     1141 ccggaaccgt agccgcagcc atcgtcgatc acgccatcgc agtcgtcgtc gagcccattg
     1201 cacgactcct cacttggatt ggcacaatct ctcgggattt cccgagctgt tgcgatagcg
     1261 acgatcgata ccaggtcggg cgcctctacg agagcgggct gttcctccgc ccgctcgacc
     1321 tctcgcgccg tctcgctgct gggtccgcct gcgcctgttc cggagcacgc gagcaagagc
     1381 gccgatgcgg cgatcgcgag tcgtttcctc atccggcagg cactatagcc gaggtagcgt
     1441 acaatgcggg ggcaatcgtg gaagcactcg agcaagcaaa gcaagccatt gcccagcgca
     1501 ccggaagacc agaggtcgcc atggtcctgg gatcggggct cggtgactac gctgacagcc
     1561 tgggaggagc gcgcgccatt ccgtacggag aggtacccca catgccgctc tcaggcgtgt
     1621 cgggacacgc cggcaatttc gtagtcgggg agaaggccgg gaagccgatc atcgtgatgc
     1681 aggggcgaac gcatctctac gaaggtcact cggctcggga tgtcgtattc ggtgtacggc
     1741 tgatggcgtt gcttggagct aagactctga tcgtcaccaa cgcagccgga gggatctcgc
     1801 cagagctgca ggccggggat ctgatggcga tcaccgacca actgaacctc acggggcgaa
     1861 actgcctgac cggcccaaac gaccagcgac gaggaccgcg attcctcgat atgaccagag
     1921 ccttcgaccc ggcgctcatc gagagcgcga gtagcgtttc aagccggctc gggttcacgc
     1981 tcaagcgcgg cgtatatgcc ggcttgctgg gtccctccta tgaaacgccg gcagaggttc
     2041 gaatgctcgc gacgctcggc gccgatgcgg ttggaatgag caccgtgctc gaggtactcg
     2101 ccgcgcgtca catgggtctc cgcgtcctcg gtgtctcgtg catctcgaat ctcgcggccg
     2161 gaatcgcaga gggccctttg agccacgagg aggtgacgga aaccgcgcgg cgtgtccgtt
     2221 cgcggttcga agcacttctg tcgggtgtgc tggagtcgct ctgatggacg agaaggcatg
     2281 ggatgcgctc aaacgaagcg ccatggaagc tcgagggaga gcctatgcgc cctactctgg
     2341 attcagcgtg ggcgcggcca tccggaccga gagcggtgag gtgttcatcg gctgcaacgt
     2401 ggaaaatgct agctacggcg cgacgatttg cgccgaacgg gcggcattgg cagccgcggt
     2461 cgcggcaggg gaacgcgagc tagtggctct agcgatcgcc tcggacgcac acgcacccat
     2521 cccgccgtgc ggaatctgca ggcaatgctt ggccgagctc gggacaaacc ttcccatccg
     2581 gagctacgcc ggcgacgcgg agctcgagta ccagctctcg gcgctcttgc cggatccgtt
     2641 tggccgcgaa cagctcgact gacacgcgat cagtcgaggc cctgcccgat tcggcgaagc
     2701 ttggcctcaa cgtcggcgcg atcgatcgcc tcgcgatcgg cgagctcgag gtagcggccg
     2761 tactggacga cggcgtcctg acgtttcttc tgggcgaagt agatgtcccc ggtcaagcgg
     2821 tgcgcatcgg ccaaccagcc tttgtcctcg ggatccggat ctccaagcga ggtggcggta
     2881 gtgagggagg tgagcgcctc ctcacgcctg ccctcatcga gctgaagacg tcccaagcgg
     2941 taccaccagt atccgtgctc gggagcccca gcaacggctt tctcccagtc cgagatcgcc
     3001 tcgcccattt gaccgagctg gtaatggcac tcgcccatcg cagcccaggc ttcgattcgg
     3061 ctcggattga gctcaatggc cttgttgaga tccgccaggg cgtcgcgcac cgtgcctgcg
     3121 cgaatgcgaa tgcgcgctct gagccagtaa gcgtccccaa gggtcgggtc gagcttcagg
     3181 gtagcgtcga gctctctcag cgcagaggtc atttcgttcg cctcgagggc gacccaggcc
     3241 acgtacattc gatagaggcc tttgtcggtt tctagacgcg acgcgcgtag aaagtactgg
     3301 cgagccgact ccaactggcc acgctccagc tcgatccgac cgagatagtg ttcggcctcc
     3361 gccgagtacg gctgagcggt gagcacttcg cgcaagagct tctcgccttc gtcgagctgg
     3421 ccggtgagca ccaatacggc gcccaagcga gatttgaggg cggcatcctt tggtgattcc
     3481 tgcagagctt cgcggtagcg cgaggccgca gcgtccatgt cgccacccgc ctcggcgagc
     3541 ctgcctcgct ccaaaggaag gccagggaac ttggcatcga gctcctgaat ctcgtcgagc
     3601 tcggctgcgg cctcttcgag catccccttg cgccggtagg ccaccgcgag gccgaaccgc
     3661 gaagccgaat ccccgggatc catttcgagg gcggcaagga actccttgac cgcatcgtcg
     3721 acgcggttgc gctgtagctc cgcccatccg atcacgcgcc gaacctccgg ggtgatctcc
     3781 acgttcttct gtgcctcgag gagcaccgca accgcctcgc ccgggcgttt ggtggagctg
     3841 tagtgctgag caagcgccat gtacgggctg aacgacttcg gcgcgagcac gatcgcgcgt
     3901 cggaaatgcg tcaccgcagc cttgctgtct ccgacattct cggcgacctt gccctgcaag
     3961 agctcgacct cggcgctgac cggctcatcg gtttcgagat cggcgagcag cttcttcgcc
     4021 tcctctgcct tgttcatggc gaggagcgcc tcggccgcgc ccagtttcgc ttcgaccagc
     4081 acttttggct ttccagtcgg atgcatcacc gcgccaggcc ggatatcgga cccgaggacg
     4141 gtctgaaagc gcgcgtaagc gtcctgataa gccccctcga gcaagacgag gcgtgctgcg
     4201 ccgagcgtgg cgttggcgtt gctcgtgtcg agctcgatcg ccttgtcgta catctcgcgc
     4261 gcggcgccga ggcggcctcg gctctcctct atccttgcga tccgcgtgag cgccgcagac
     4321 tgatcgctgc gcgccacctg gagagcgacg ccctgctcct cggcaagtcc tgccggaatc
     4381 tgcaaaagcg cataggcttc gtcggccttg ccttcgtcga tgagcgagtc ggccacggct
     4441 acgcgcgcag cagcgtgatt cggactgagt cggagcgttg ccctggcggc cgcgacggcc
     4501 gcgcccgtat cgccgaggat ttcggaagag cgggccagcc cccattgtgc gcgtgcgctc
     4561 tcgtgcttct ggagtgcgcg ttcaaacgcg gcgcgcgcgg cttgcccatc gcgctctctc
     4621 agccagagct cgccggcgac caggtcgacg taggtgtcgt tgggatcatc ctgcgcggcg
     4681 agcgcgagtt cggacttggc agtggcattg tccgcgttgc gcaatgcatc cgccgcaagg
     4741 gcgacgtgga ctctcgtagc cgtgtcaccg cgacgctgaa gatgcaggcg taagctgtct
     4801 gcgacctcag cgctcgtcgc atcctgtccg tagcgcactt ggtggtacga ctcgtgcagc
     4861 agggctcgag cgacgaggga gcggttcaag ctcgcgtcac ggcgagccgc ttgcagatgg
     4921 gcaagggctt gctggctggc cgcgtacgtg tcggcagcga aaatcatctc cgcttcctcg
     4981 atgctctgcg cgactacgag ctcgtctccg gcggccggaa ggaaaggctc cagcagatga
     5041 atcccgaaaa gtccgtactg cgtggtgccc gaataaaccc ccgcaccgag tacgagagcc
     5101 atgacaccga gcgcggccac cgccttcatc agccacggag ggcgccgtac ggaaatcttg
     5161 agcttgccct tctgcggagc gacctgctcc ggaccggtgc cggccggccg aggcatccgg
     5221 tgcacgccgt ccgcgcgctc tccgggaagc tccgaaaact cgagctcgtc ggcatcgtcg
     5281 ctgatacgct tgtcggcgtg ccgcgcaacc tcaggcgctg cgcccggagc gccgaaggca
     5341 tgaccgagcg gtggcggcgg cccaggagcc ttcgcgggct cttccagatc catgtcgagg
     5401 tcgagatcgc caccgtcggg caaatcgagc tcgatggggc cgcctccgtg gacgcgctcg
     5461 gggccctcga ggtcgaggtc catgaagtcg tctcgggcta cgggaaggtc ctcttccccg
     5521 atcgccatcg gaagctccga gtcgagcgaa attggcgaag gcaccgccga gcctcggcgc
     5581 ggcgtcggca gctcggcgtc gctcagtgcc atgggcaagt cgacctcatc gccaacctgc
     5641 tcgatacccc tcgcacgcgt ggcgcccttt gcaaccggta ggtcgatctc gcctccgtag
     5701 gaatgcgaca tcggtgcagg aaggtcggtg cctccgggcg agtcgagggg cgccggcaaa
     5761 tccatgtcgg caaaaggatc gaactccgaa gctcttgggt ccgtcttcgg cgcggggagg
     5821 tccgccaaac ctggaacctc aaaaggatcg aagtcgagcg agcgtccccc tttcttcggt
     5881 gcgggcaaat cgccctgctg ccggccggcg aaaggcgcgg gaagatctgc gagctcgcga
     5941 acgccagcag gcgtagggag atcgatttct tctacgtttg ccgacaaatt cgaaggctgc
     6001 ggcttgatga ccgcaaccgg cggcggtggc ggtccggctg ggggggagct cggcccaagc
     6061 ccaacctggg ttggtttgcg tcgcggaggt ttcttggccc ccggagccgc gcccccaccg
     6121 ctcctggcgg tcgagccgtc gcgatgaacc tgaaaactat cgctgcactt cgggcagcgc
     6181 atccgaagcc ctttgtcagg caatcggtgc tcgtccacgt cgtaagacgc atcacatgac
     6241 gggcaattaa ccttgatcat gatcccccac tgttccccgg gatcaaagat aacacgtcag
     6301 cggctcgact cataaaccct cgtagtcgga ttccgatcgt agcggaacca agacggaatg
     6361 gatgtgcatg tcctaccttg acgccaaagg agctggagga ctaccttcca tcaatcgtga
     6421 agcgcaacgt tcagatcgag attgctggcg cccgttaccg catgtcttcg gacgctgacg
     6481 aagcatacct acaacgtctc gccgatatcg tgaacgagcg cgtaaacgcg ctcggcccca
     6541 aagccgcccg agcggcttcg cccgcacagt tgcttgctgt cgtggcgctc agcctggcgg
     6601 aggatctcga cgcgtccgag cggcgtcgcg gcgatctcga gaccaagacg cgtgagctgg
     6661 tgagcaacgc gattcgacgc atcgacaatc gactgcaggc ggatgccgag ctcgcccagc
     6721 aaatcgagcc gtgagcaaag agaagacgga gcgcctcgag agccagctcc gtcagcgcgc
     6781 gaaaatggcg ctgcgcagtc gaatgcgcgc ggtgcggggc gccctccccg attcggcgtg
     6841 cgacgcacgt tcgaagaaga tcgatgcgcg tctctgggag ctgactgagc tcgagcgtgc
     6901 atcgaccgtg ctcgcgttcg ggtcgattgg aaacgaggtg cgcactcgtg cttttatgga
     6961 gtcggcctgg gccaagggga aacgcgtagg actgccacgc gtcgtcggcg atgagcttct
     7021 cttgcatctc gtcgaacgcg gaacggcgct cgcgaagggc tcgtactccg tgccggagcc
     7081 agcggaagat gcgcctcgat tggaaccaat cgatgtagat ttcgcgctga tccccgcctt
     7141 ggcagtcgat tcgaggggct accgaatcgg ttacggggct ggctactacg acagactgat
     7201 ccctcttctg aagcgcgcct gcacctgcgc agtcgcctac gacttccaat tgatttcaga
     7261 agttccggag ctgcccttcg acgtcgccgt cgacctcgtc gtcaccgatg agcgggtgat
     7321 tcgcagccgc tgagcgtcgg ggcgatcgtg ggcaggccgg atggtatagg tcgagcgtgg
     7381 tcgccgaggc agcacaacga aagctcgaca agctcccgcc ctcgcctggc gtctacgtat
     7441 ttcatggggc cgatggaaag gttctctacg tcggaaaagc gcggagtctg aggagccgcg
     7501 ttcggagcta ttttcagcca gggtcgagtg acctgcgcgc cttcgtgagc aggctcgagc
     7561 gcgagctagt cgacatcgac acttacgtca cacacaccga caaagaagcc gcgctgctcg
     7621 agaaccagct gatcaagtcg catcagccga agtacaatgt gaagcttcgt gacgacaagg
     7681 aatacctatc ggtacgactc gatacgaaga aaccatggcc gagactggag gtcgttcgac
     7741 gcccgaagcc cgacggcgcc cagtacttcg gtccgtatca ctcggcgacc gcggcgcggc
     7801 agacgctgcg actggtgaac cgatacttcc agcttcggac ctgcacggac acggagctgc
     7861 ggctccgatc ccgtccctgt cttcagtatc agatcaagcg ctgcccggga ccctgcgtgc
     7921 tcgacgtcga cgaaacggag tatcgggccc aggttaccaa cgtagcccgc ttcctcgagg
     7981 ggcggcacgg cgagttggtc cgcgatctcg atgatcgaat gaaggtcgcg tcggacgagc
     8041 tggactacga gcgggcggcc atctatcgcg accaacttcg cgcggtagag cgcgctcgcg
     8101 aagagcaacg cgtagcgggc gtgcagaagt cggaccagga cgtgatcggt tttcatcgag
     8161 aaggggacca agtcgaagtc gcggtcctga gactgcgcgg cggaaggctc ttcggcgtgc
     8221 ggacgcttcc gcttcgccgg gtcgccgtgc ccaacgacga gatgctaggc gcgttcttgc
     8281 gtcaacacta cgccgaccga ccggacttgc ccgacgagat cttggtgccg acgagtatcg
     8341 aaatgagcga agcgctcgag gagcttctca gcgaaggccg gaaccgtcgc gtacgcatcg
     8401 tcgaacccaa acgcggtgca aaagccaagc ttctgacgct ggcgcacgag aacgcggagc
     8461 atgcctacgg ggagaaagaa cgcgcgaagg aggacgtcga aagccgcctt gcggatctgc
     8521 agaagcaact tcgcttgtcg gtccttccgc gacgaatcga gtgcgtcgac atttctcaca
     8581 gtggtggcga ggagacggtc gcggtcttcg ttacgctgca ggacggaaca ccggcaaggg
     8641 agcgttatcg aagctttcgg gtgaaaggcg tgggcggagg ggacgactac ggagccatgt
     8701 acgaagtgct gatgagacgt ctcagacgtg ggagaaacga ggaagagggc tgggagctcc
     8761 cggacttgct cgtcgtcgac gggggaaagg gccagctcgg tgtagcggtt cgtgcccgcg
     8821 aagacgtagg agtcccggag ctcgaggtcg catcggtcgc caaaccgcgc gtgacggtca
     8881 tgggggaaga agaaggcgac cgcgttttcc gtccggggca aaagaacgcc attgccgtgc
     8941 gcgccaattc ggcactctcg ctactccttc tcgcccgcga tgaaacccac cgggcctcca
     9001 atgtcctcag aaagaaggtc gggcgtaaga gacgtctcag aagcgagctc gacggagtgc
     9061 ccggagtggg gcccaagacc agaggcaagc tgctccgcaa cttgggctcc ctgcgcgaag
     9121 tgatggcggc gaccgaggaa gagctgatcg aagcgggcgc aactcggaag caggccgagg
     9181 cgatccaacg agccctcgga actcagacct cgcccccccc cgagaccgag gccgccgaag
     9241 acaccgccat cgacaacgcg tttcaacccg attaggcgcc ggcaccggcg ctatgaagat
     9301 cggagggcgg ctcgcgacgc acgtgctcgc gattgggacc cgctcggctg tctgcgtacg
     9361 acgcgatacg tctggcctcc cgcccaatcg ctgtgccgcg catcccgagc cgccctcctt
     9421 tgtacacggc taagcactga tgctgacacc gacgctacca ctggcactga caccggctct
     9481 ggcactggca ctggcattgg cactggcacc gaactatcgg gccgccggca gcctaacctc
     9541 aaatgctgcc ccgccctgct cccggttctc cgcctcgatc gaaccgccca gc
//
LOCUS       DASRZF010000188         4210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2881341, whole genome shotgun sequence.
ACCESSION   DASRZF010000188 DASRZF010000000
VERSION     DASRZF010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2881341"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /gene="dacB"
                     /locus_tag="VFG22_08305"
     CDS             <1..954
                     /gene="dacB"
                     /locus_tag="VFG22_08305"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HET6416286.1"
                     /translation="EAASFRAAISAFSVERNSYVVHIGPGPKVDAPGRVRVLPEEYVQ
                     IENRTVTTAGGPPRPRIDEKLTDDGRLRILVDGTVPQQVRTLYYRRRIPEPKVHAATL
                     LVRALRKAGVGGELAVVYDLAPQPQPLIADLASPPLSILLYSVGKWSDNFSAEMLLKI
                     MGAEAERPGTSARGVIAVREELERMGVNTQGLVMVNGSGLFDGNQVAPSHLTATLVAA
                     YHDPAIRAEYVAQLAVAGADGTLQSRLQDLPRARMVRAKTGTLRDVVALSGYVLGAPE
                     RSLAFSFLANGVAGRQGAARNLADDIVRALANYAVQPSADH"
     gene            1011..3125
                     /locus_tag="VFG22_08310"
     CDS             1011..3125
                     /locus_tag="VFG22_08310"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET6416287.1"
                     /translation="MPKALFVLAVLLLASCAGSPKPTTGAPTPRAEPPVAATHPHSVT
                     LHGDTRVDDYAWLQDKGSPEVISYLEAENAFADARMESTQELQDSLYEEIVGRIQETD
                     QSAPYRKGAFEYYQRTIAGKQYPIHCRRVPGKEDSEQVLLDMNALAEGRPFLGLGVYE
                     VSPDGNYLAFSLDETGFRDYTLQVKDLRSGELLGERVEKVKSLAWANDSETFFYTVDD
                     ASKRPHEVWRHVLRRSAADELVYREDDERFFVAVWRSRSGEYVFRGAFSHTTSEIGFV
                     SASKPRGIWRIVAKRRQNHEYDVEHRRDHFLIRTNDRGRNFRVVRAPVSSPGPASWKE
                     LVAHRPDVMIESLDAFDGFYVMLERERGLPYLRVSRFLDGESHRVATEEPVYALYPGD
                     NEEFASTSFRFRYESLTTPDSVFDYDVVSKTRTLVKQVEVLGGYDPARYSSERLWAIA
                     EDGTEVPVSLVYRHDTPRDGSAPMLLVGYGAYGYPYPTGFSHARVSLLDRGMIYAIAH
                     VRGGGELGKRWHDEGRMDKKMNSFTDFIDVAEHLLGARYTSSDRLAIQGGSAGGLLMG
                     AVVNLRPDLFAAVVSLVPFVDVLNTMLDASLPLTIGEYEEWGNPNEPDAYFRIKAYCP
                     YTNVRAQAYPTMLVRTSINDSQVMYWEPAKYVAKLRALKIDDNPLLFKINMEAGHGGA
                     SGRYDYLKETALDYAFILTHAH"
     gene            complement(3371..>4210)
                     /locus_tag="VFG22_08315"
     CDS             complement(3371..>4210)
                     /locus_tag="VFG22_08315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6416288.1"
                     /translation="VQESDRAVHRLAGDFADERDIVGDHVRMTPTFKRYLTHVRDAQK
                     AQQRAKNRFVAANLRLVVSIARRYNRGRLPLIDLIQEGNIGLMKAVERFDHNRGYRFS
                     TYASWWIRHAISRALADKGRAVRIPVHMLDTYNRVARATQAIATRTGKEPTPEELEKE
                     TGIAAEKLEKIKGYWAETPFSLDRPVNDEDGRKFIDFLEEENVPTPYEQLVSRKWSDE
                     VRRLLDTLTPMEARILRWRFGLDDEDELTLKEIGDKYNLSRERIRQLQEQALGKIRRQ
                     IKE"
BASE COUNT          783 a         1295 c         1325 g          807 t
ORIGIN      
        1 gaagcggcgt cgtttcgcgc cgcgatctcc gcattctccg tcgagcgcaa cagctacgtc
       61 gtgcacatcg ggccgggtcc gaaagtcgac gcacctgggc gcgttcgtgt gctcccagag
      121 gagtacgtgc agatcgaaaa ccgcacggtc acgactgctg gcggaccacc gaggcctcga
      181 atcgacgaga agctcaccga cgacggacgc ttgcgcattc tcgtcgacgg aacggttcca
      241 cagcaggttc gcacgctgta ttaccggcgc cgcattcctg agcccaaggt ccatgcggca
      301 acgctcttgg tgcgggccct caggaaagcc ggagtgggag gcgagctcgc ggtcgtctac
      361 gacctggcgc cccagcccca gcctttgatc gcggacctcg cttcgccgcc actcagcata
      421 ctgctctact cagtcggcaa atggagcgac aacttcagcg cggagatgct tctgaagatc
      481 atgggcgccg aggccgagag acccgggact tcagcgcggg gcgtgatcgc cgtgcgcgag
      541 gagctcgaga gaatgggtgt gaacacccag ggactggtca tggtcaacgg ttcgggccta
      601 tttgatggga atcaggtcgc tccttcccat ctcacggcga cgctggtagc ggcgtaccat
      661 gatccggcga tccgagccga gtacgtagcg cagctggcgg tggccggtgc ggacggcacg
      721 ttgcaatcac gactgcagga tctcccgcgg gcgagaatgg tacgcgccaa gaccggaacg
      781 ctgagagacg ttgtcgcctt gtccggatac gtgctcggcg cgccggagcg ctccttggcc
      841 ttcagcttct tggctaacgg tgtcgcgggg cgacagggcg ccgcccgcaa ccttgccgat
      901 gacatcgtca gggcgctcgc aaactacgcc gttcaaccga gcgcggatca ctgaaaccgc
      961 attcgacacg gtcgactgcg tctccgttta gaggtgctag accttcgccg atgcccaagg
     1021 cccttttcgt tctcgcggtg ctcttgctgg cgagttgtgc tggctctcca aagccgacga
     1081 ctggagcgcc aactccccgc gcggagccgc ccgtcgctgc gacccacccg cattccgtta
     1141 ccctgcacgg tgacacgcgc gtagacgact acgcctggtt gcaggacaag ggatcgcccg
     1201 aggtcatctc ttacctcgag gccgagaacg cgtttgccga tgcacgcatg gagtcgaccc
     1261 aggagctaca ggactcactc tacgaagaaa tcgtcggacg cattcaagag accgatcaga
     1321 gcgcgccgta tcggaaggga gcattcgagt actatcaacg gacgattgcc ggcaagcagt
     1381 acccgatcca ttgccggcgc gtgccgggaa aagaagatag cgagcaagtc ctcctcgaca
     1441 tgaacgcgct cgccgagggt cggccgtttc tcgggctcgg cgtgtacgag gtgtcgcccg
     1501 acggcaatta cttggcgttc tctctggatg aaactgggtt tcgggactac acgcttcaag
     1561 tcaaggacct gcggtctggg gagttgctgg gcgagcgagt cgagaaggtc aaatcgttgg
     1621 cctgggcgaa cgatagcgag acgtttttct acacggtcga cgacgcaagc aagcgacccc
     1681 acgaagtctg gcgtcatgtg cttcgccgga gcgccgcaga cgagctcgtc tatcgggaag
     1741 acgacgagcg gttcttcgtc gcggtctggc gttcccgaag cggagagtac gtatttcggg
     1801 gagccttcag ccacacgacc agcgaaatag gattcgtttc tgcttccaaa ccccgcggca
     1861 tctggagaat cgtagcgaaa cggagacaga accacgagta tgacgtcgaa caccgacgtg
     1921 atcatttctt gatccgaacg aacgaccggg gacgcaattt ccgcgtcgtc cgggcgccag
     1981 tttcgagtcc tggacccgct tcgtggaaag agctggttgc ccaccggccc gatgtgatga
     2041 tcgaaagcct ggacgcattc gacggtttct acgtgatgct cgaacgcgag cgcggccttc
     2101 cctaccttcg tgtctcccgc ttcctcgatg gggaatccca tcgagtcgca acggaggagc
     2161 cggtttacgc actgtacccg ggcgacaatg aagagttcgc ttcgacttct tttcggtttc
     2221 gctacgagtc cctcacgacg cccgattcgg tgttcgacta cgacgtagtt tcgaagacac
     2281 gcacgctcgt gaagcaggtc gaggtgcttg gcggctacga ccccgcccgg tactcatcgg
     2341 agcgcctttg ggcgatcgct gaggacggga cggaagttcc ggtctccctc gtctaccggc
     2401 atgatacgcc gcgtgacggc tcggctccca tgctgctcgt gggctacggc gcgtacggct
     2461 acccgtatcc gaccggattc tcgcacgcgc gagtgagcct tctcgatcgg gggatgatct
     2521 acgccatcgc ccacgtccga ggtggcggcg agcttggcaa gaggtggcac gatgagggcc
     2581 gcatggacaa gaagatgaac agcttcaccg atttcatcga cgtcgccgag cacctgctgg
     2641 gtgcgcgcta cacatccagc gatcgtcttg cgatccaggg cggaagcgcc gggggcttgc
     2701 tcatgggcgc agtcgtcaac ctccgcccag acctcttcgc agcggtcgtc tcgttggtgc
     2761 cgttcgtcga cgtgctcaat accatgctcg acgcaagcct tccgctcacc atcggtgagt
     2821 acgaggaatg ggggaatccc aacgagcctg atgcctactt ccgcatcaaa gcgtattgcc
     2881 cttacacgaa cgtccgggcg caggcctacc cgacgatgct ggtgcgcacg tccatcaacg
     2941 acagccaggt catgtactgg gagccggcaa agtacgtcgc caagctgcgc gcgctcaaga
     3001 tcgacgacaa tcccctgctc ttcaagatca acatggaagc gggccatggc ggggcttcgg
     3061 gtcgctatga ctacctgaag gaaacggcgt tggactacgc attcatcctg acgcacgcac
     3121 actagctgca accggggcgc cgtggaacac gacacggggt tccgacgaac ggctcgggat
     3181 gggcggcaca gcgattgggc gggaggcgag acgtagggaa acttggagag acggccgagc
     3241 gggtcccaat cgcgagcacg tgcgtcgcga gccgctctcc gttcgaacgg tgccatggtc
     3301 ggtgtgtgcc cggaattgca aacgccggcg gggaatccct cgccggcgtt cgacgttcgc
     3361 gagcctcagc tcactctttg atttgtcggc gaatcttccc cagcgcttgc tcttgcagct
     3421 gccggattcg ctcgcgggac aggttgtact tgtcgccgat ttctttcagc gtgagctcgt
     3481 cctcgtcgtc gaggccgaag cgccagcgta gaatgcgggc ctccatcggg gtgagcgtgt
     3541 cgagaagcct tcgcacctcg tcgctccact tcctcgagac gagctgctcg taaggcgtcg
     3601 gcacgttttc ctcttcgagg aaatcgatga acttgcggcc gtcctcatcg ttgaccggac
     3661 ggtcgagcga gaacggggtc tcggcccagt agcccttgat tttctcgagc ttctcggccg
     3721 caatgccggt ctctttctcg agctcctcgg gcgtcggttc cttgcctgtg cgggtcgcaa
     3781 tggcctgagt cgcacgagcg acgcggttgt aggtgtcgag catgtgcaca ggaatgcgaa
     3841 ccgcccgacc cttgtccgcg agtgcgcgac tgatcgcgtg gcgaatccac caggacgcat
     3901 aggtgctgaa tcggtaacca cggttgtggt cgaatcgctc gacggccttc atcagaccga
     3961 tgttgccttc ttgaatgagg tcgatgagcg gaagccggcc acggttgtag cggcgggcga
     4021 tcgaaacgac tagacgcagg tttgctgcca cgaagcgatt cttcgctcgc tgctgggctt
     4081 tctgcgcatc gcgcacgtga gtgagatacc gcttgaacgt cggcgtcatg cgcacgtggt
     4141 caccgacgat gtcacgctcg tccgcgaagt cgccagcgag ccgatggacc gctctgtcgg
     4201 actcctgcac
//
LOCUS       DASRZF010000189         2984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_129464, whole genome shotgun sequence.
ACCESSION   DASRZF010000189 DASRZF010000000
VERSION     DASRZF010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129464"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(357..1277)
                     /locus_tag="VFG22_08320"
     CDS             complement(357..1277)
                     /locus_tag="VFG22_08320"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET6416289.1"
                     /translation="MSAQSKMGTEGRALAGSGYVLVTAAYNEERHIADTLRSVADQRL
                     LPCRWVVVSDGSTDTTDQIVRAWAERHPFIEFIRVEKAEKHDFAAKVHALRQGFARLA
                     DVDYDFIGVLDADLSFQPDYFERLLEHFAAEPELGVAGGNIEQLVDGAIVPRVKDLNS
                     VAGAVQFLRRECFEQTGGLPALKHGGEDAAMEIAARMHGWQTRTFPELKVLHFGLVGA
                     GAGGALRARFKWGRMNFNLGYHPLFQLARSAYRVGEKPYLFGSFAELAGYAAGRARDR
                     QPSVDGDVVRYLRREQLAKLRHPLNARRSK"
     gene            complement(1280..2002)
                     /locus_tag="VFG22_08325"
     CDS             complement(1280..2002)
                     /locus_tag="VFG22_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416290.1"
                     /translation="MQAEPMLSFGVPVRSGARFLRRLLTSVEAQDFDYFEVVLEPTSA
                     RRCVEANPDAVGVTTLWRYVDDEVNQVLLNDSGIRVDSKSVQRRISRMLWCLQADFGA
                     DPIYSALRSSMLKGIGLLPIGPWTDRVPALELAIDGPFCQVHHRRATRRNAREPAKVR
                     LARHHSAIKEVEHATEVRLAPRWAMYRGLAKVVINSFLEPADRVLGTTLVISFGGLHH
                     ARALGERALKLVGQGQAAATTA"
     gene            2153..>2984
                     /locus_tag="VFG22_08330"
     CDS             2153..>2984
                     /locus_tag="VFG22_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416291.1"
                     /translation="MNNDVRDMSDMFTEYGATNIHPVAAVFMLFMIVMVLSSRRSRVI
                     LAFLLVCVFMPMMQRIVVGGLDFNMMRLLLVVAWGRVFVRGEYRGFKPGKLDYLFVAW
                     TASLAIFHVLRVGPGGIVYRLGTSFDALTVFFLFRVLVRRREEVLLIARELAWIVIVL
                     GGFMAYEMATRLNVFSIFGSAPLISVIRDGKVRCQGPFSHPILAGTFGAVLVPIFVGV
                     FRGQRKERKLMAAALLFATITTVASGSSGPIITWAVGVFGWGVWLVRSRTRAIMWSLA
                     G"
BASE COUNT          565 a          957 c          875 g          587 t
ORIGIN      
        1 gtcccgctcg agcgacgacg gcagtccgct gaacgcgttc gcccaatccg acccgtcctt
       61 cgcgcctccg ccaccgggac gtacgtaccg cgcccgtgtg ctgcacccgc tgaagctgaa
      121 cccctcttcg cagcagaacc ctgtcgtgtg taccgagccc ccacaaacac aggacatgct
      181 caacgcctct ccagtcggac agtcgttcag gagttcgctt ggtcgttcca aaccgcctcc
      241 ggtccgcggc ccgtcagaag caccatcgct agaacgcgtg catgcagtcg acatcaagct
      301 cagaaagagc aacgcgaaac agttccgcat gaagccacca tacccgcaat ggccgactac
      361 ttcgagcggc gagcgttcaa cggatgcctg agcttggcga gttgttctcg acgcaggtac
      421 cgtacgacgt cgccatcgac ggagggctgc cggtcgcgcg ccctgcctgc agcatatccg
      481 gcgagctccg caaagctgcc gaacaagtac ggcttctctc cgacgcggta cgccgaacga
      541 gccaactgaa acagcggatg gtatccgagg ttgaagttca ttcgccccca cttgaagcgc
      601 gctcggagcg ccccaccggc gccagcgcct actagcccaa aatgaaggac cttcagctct
      661 ggaaaggttc tcgtctgcca gccgtgcatt cgcgccgcaa tttccatggc agcgtcttca
      721 ccaccgtgct tcagcgcagg caagccgccc gtctgttcaa agcattcccg acgaagaaac
      781 tgaaccgctc ccgcaaccga gttgaggtcc ttcacccgcg gtacgatggc tccgtcgacg
      841 agctgctcga tgttgccgcc cgcaacgccg agttcgggct ctgccgcaaa atgctccaga
      901 aggcgctcga agtaatccgg ctgaaaagaa agatccgcgt cgagcacgcc gatgaagtcg
      961 tagtcgacgt cggcgaggcg agcgaaacct tgacgtagcg catggacttt tgccgcaaaa
     1021 tcgtgcttct cggccttttc tacgcgaatg aactcgatga aggggtggcg ttcagcccat
     1081 gcgcgaacga tctgatcggt ggtgtccgtg gatccgtcac tcacgaccac ccaacggcat
     1141 ggcaacagcc gctgatcggc gaccgatcgg agcgtatcgg caatgtggcg ttcctcgttg
     1201 taggcggcgg taacgagcac gtatccgctt ccagcgagtg cgcgtccctc ggtccccatc
     1261 ttgctctgag ccgacatggc taagccgtag tcgcagcagc ctggccctgc ccgacgagct
     1321 tgagcgcgcg ctcacccaag gcgcgcgcat ggtgcagccc gccaaaagag atgaccaacg
     1381 tcgtgcctaa cacccgatcc gcaggctcca ggaaagagtt gatcacgacc ttcgccaaac
     1441 cccgatacat cgcccagcgc ggcgccaagc gcacctcggt cgcatgctcg acctccttga
     1501 tcgccgagtg atgcctggcc agtcgcacct tcgccggctc gcgagcgttt cttcgcgtcg
     1561 cgcggcgatg atgcacctgg caaaacggtc cgtcgattgc caattccaga gcaggcacgc
     1621 ggtccgtcca ggggccgatg ggcaagagcc ctatcccttt caacatgctg ctccgaagag
     1681 ccgaatagat cgggtcagct ccgaaatcgg cctgcaaaca ccagagcatg cgcgagatgc
     1741 gccgctgcac cgacttcgag tctacgcgaa tccccgaatc attgaggagc acctgattga
     1801 cttcatcgtc tacgtagcgc caaagggtgg tcaccccaac cgcgtccggg ttggcttcga
     1861 cgcagcgccg tgcggaggtc ggctcaagga cgacctcgaa atagtcgaaa tcttgcgctt
     1921 ccaccgaggt gagcagtcgc cgaagaaatc gcgcgccgct tcgcaccgga accccgaagc
     1981 taagcatagg ttcggcctgc atcttcgtac tagcctgcat catggccgag gacacgctat
     2041 tgtgaccaca gagcctcatt gtgcacactg ggccccgcgt ccgcaagcgc cgctgaggga
     2101 gtcctgggca tcctatgcat gacgacgtga gctggttccg gagggaggcg ctgtgaacaa
     2161 cgacgttcgc gacatgtctg acatgttcac ggaatacggg gcgaccaaca tccacccggt
     2221 cgccgccgtg ttcatgctgt tcatgatcgt catggtgctg tcgtcgcgcc gttcaagagt
     2281 cattctggcg tttctcttgg tgtgcgtctt catgcccatg atgcagcgca tcgtcgttgg
     2341 aggtctcgac ttcaacatga tgcgactgct cttggtcgtc gcctgggggc gagtcttcgt
     2401 gcgcggcgag tatcgcggat tcaaaccggg caagctggac tatctgttcg tcgcttggac
     2461 ggcttcgctc gcgatcttcc acgtgttacg cgtcggccct ggcggcatcg tctatcgatt
     2521 gggaacgtcg tttgacgcgt tgacggtatt cttcttgttc cgagttctcg tcagacgacg
     2581 cgaagaggtg ttgttgatag cgagagagct cgcgtggatc gtgatcgtgc ttggcggatt
     2641 catggcctac gaaatggcca cccggctcaa cgtgttctcc attttcggca gcgcgccact
     2701 gatctcagtg atccgcgacg ggaaggtgcg ctgtcaaggg ccgttttcgc atccgattct
     2761 cgccggaacg ttcggcgccg tgctggttcc catcttcgtc ggggtgtttc gaggccagag
     2821 gaaggagcgc aagctcatgg ccgcggccct tctctttgca accatcacca ctgtggcctc
     2881 aggctccagt ggcccgatca tcacctgggc tgtgggcgtt ttcggctggg gggtgtggct
     2941 cgtgcgaagc cgcactcgcg cgataatgtg gtctctggca ggcc
//
LOCUS       DASRZF010000190        20304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1852578, whole genome shotgun sequence.
ACCESSION   DASRZF010000190 DASRZF010000000
VERSION     DASRZF010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 20304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20304
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1852578"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VFG22_08335"
     CDS             <1..357
                     /locus_tag="VFG22_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416292.1"
                     /translation="GRIAGRRLLGDTLQYARGTWYNSAKLMDIEYTTAGLVNWKLERE
                     ENWFHEERGKVNSTTRIVAQDDRVVGFNMLGRRWDHSVLARWIEERRKLDWVLQHLND
                     AAFDTEFVAPLRLPRR"
     gene            359..1489
                     /locus_tag="VFG22_08340"
     CDS             359..1489
                     /locus_tag="VFG22_08340"
                     /inference="COORDINATES: protein motif:HMM:NF024585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HET6416293.1"
                     /translation="MDQQGQNGLLATYRSGWRNRSPIGIAISVLLVSFYVVLFWEHKL
                     QAAAGVAPFTAFSEALGLRNRWYLYGFLYSIAMSAGAVFYLRRHGNSRYNRFRITVNV
                     AIQIMLGFTLPFIMPLFGGKELYFSYFWPLKYDYFYPQTLADVPLYFALYTVLGSLIA
                     APILAVFYGKRWYCSWVCGCGGLANTFGDPWRHLTSKSTKSWRFEQLSIHLVLALAVA
                     TTFLIGLDSLIGSEHPTFHRIVAGEDWQDWSVKSFYGFAVGAVLAGVIGTGLYPVLGP
                     RVWCRNFCPMAAVLGWIQKVGRYRIKVKPDMCISCGNCSTYCEMGIDVRSYAQTNQSF
                     TRASCVGCGMCAHMCPRGVLKLESSWQRAEDKQREKLSVVDF"
     gene            complement(1493..2005)
                     /locus_tag="VFG22_08345"
     CDS             complement(1493..2005)
                     /locus_tag="VFG22_08345"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: protein motif:HMM:NF001664.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HET6416294.1"
                     /translation="MSTKVRIVQGDLCHADTDAIVNAANPRLLGGGGVDGAIHRAAGP
                     ALLRACRALPELRPGVRCPRGEARITPGFDLRARYVIHTVGPVYAGRAEDPDLLSAAH
                     RSSLALAVENGLESIAFPAISCGVYGYPIDEAAAVSAKVVREKQWDLREIRFVLYSAE
                     ILAAWNRAFA"
     gene            complement(2005..2415)
                     /locus_tag="VFG22_08350"
     CDS             complement(2005..2415)
                     /locus_tag="VFG22_08350"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02266 family protein"
                     /protein_id="HET6416295.1"
                     /translation="MSEGGRERRESGRATLELPIEYDRLNALLSDYTNNISRGGTFIR
                     TDRPLPLGTVLSFTIRAPELGEPLVLRGAVRWVVEAGKAESRRPAGMGIAFLYDSPQQ
                     KEAIERRLDRLMVQSLGPLAFEQLMGKPAPVDKT"
     gene            2564..4849
                     /locus_tag="VFG22_08355"
     CDS             2564..4849
                     /locus_tag="VFG22_08355"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HET6416296.1"
                     /translation="MTETDSKTSETSKEEVAPVESQATVSSYQPQVEAVPVAATVTVT
                     EPAPEPAEKTAEADTVAGEVTAVETHADTVSGESAPETGEESSAGSVAAEGAGKKKRR
                     RRRKKGGQAGASESPSDPKSAHHHAPFLHLFSGGAARKHAFSAGEEIAGRVERVEHGT
                     VIIDVFGKATAIANVDEPRDIAHAEPVVDPILAITQASEATELPEAHVPVHTPVVPAI
                     TTTPTPVVEAPTPEMAKLAQGDASSWEFQPTEVTDVPDDELGEADTVVGAQAFDPGSL
                     IDPQLAATQPPAIAVTEPVPAASSTEEVAPESEAMSEPPEDPAEADLSLLDPLPEFEA
                     PQIGAIFRGRIGGVAESGHIVIVNRLIDKAAARKALRAARDLHKRVEGVVYGFNRGGF
                     DVLVGGVRAFCPASAMSLAPIDDPNALVGHRFEFSLPPDKGGNKKSIIVSRRGILEKE
                     ARTKARERMAELQVGAKLRGKVLEVRDYGVLVDLGDGLDGLVHMSEVAWGRGVRPQDA
                     AKVGDEVDVEVLKVQPASRKDRFGRVSLSMRACQPDPLDAAKDSIRPGMPIKGKVVRT
                     TEFGAFIELQENIEGLLHISELGGSRELTHAKQAVTEGDEIHVVVERVDRQQRRISLS
                     KLSEADAAAIEAGEIEIGTAPRSLKPGTVVSVIVKRVDHSGAQVQVGGMLGKRGRGFI
                     PNRELQDVQEDKRKGVSQGDVIEVKVIGTERDGTLRCSIRARLLDDERKAVREYRKES
                     AKQGLGTFGDLLKAKLGGSSE"
     gene            4943..5014
                     /locus_tag="VFG22_08360"
     tRNA            4943..5014
                     /locus_tag="VFG22_08360"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4975..4977,aa:Val,seq:cac)
     gene            5092..6114
                     /gene="meaB"
                     /locus_tag="VFG22_08365"
     CDS             5092..6114
                     /gene="meaB"
                     /locus_tag="VFG22_08365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HET6416297.1"
                     /translation="MADLSTLVQRIQSGEMRALARACRLIDERDPRAFDLLKQVFTQD
                     GHAYVIGVTGTPGAGKSTLVDGLIRLYRQQDKRVAVLAIDPTSPYSGGAILGDRSRMQ
                     QHFTEEGVFIRSIATRGHLGGLSRSTGDVLHVLQAAQFDVVILETVGVGQDELEVTGL
                     ADTTLVVMAPGMGDDVQAIKAGILEVADVFAVNKADRVGADATAQDLQQMLSLRRVPV
                     GASKMQGHTAVSSLRKGPEAKADEWVPPILKTVAHRREGLEELIDAFARHREFLATTE
                     TGRRLREQRRIAEFRAILYQALIDEVDLRFADRVTERLQDILDGRADPYSSAEALVRG
                     ALGDDI"
     gene            6157..6933
                     /locus_tag="VFG22_08370"
     CDS             6157..6933
                     /locus_tag="VFG22_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET6416298.1"
                     /translation="MSSFVEVTHEGHVSTLTINRPDKLNALSREVLSDLGAAIDGLAG
                     NQAVRAAVLTGTGKAFVAGADIAAMKNMTEAEGRAFGALGHGVFAAIEHLRFPVIAAV
                     NGFALGGGCELALACDFIYASSKAKFGQPEVNLGIIPGFGGTQRLPRRVGPARARELI
                     YTGKMIDAAEALRIGLANAVFEPDELLAGAQKAALEIAAKGPLAVSAAKRLIRDGIDL
                     PLPAANQLEQNAFGEAFGTEDQSEGMAAFLEKRAPTFKGV"
     gene            complement(6942..9635)
                     /locus_tag="VFG22_08375"
     CDS             complement(6942..9635)
                     /locus_tag="VFG22_08375"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HET6416299.1"
                     /translation="MSRILERVHFPLLAFCAALIACGGSKTAVEPTPMPVRAPEGKLP
                     DGVRPTAYRLNLQIIPDGDSFHGTADIDVELDAPSSIIWMHGQDLDVTSIHARHGSAR
                     VQADWEQQTSDGVVRVVFTDTLPQGRSTLEIEYSAKFDTPLRGLYRVESGDDSYAFTQ
                     FESISARLAFPCFDEPRFKTPFEVTLTVPDDQIAAANTPIERATRLPNGLQRVSFLPT
                     PPLPTYLVAWAVGPLDVVIGPTIAPTKERSFPIPLRGLAAKGKGEQLRFALQRTGKFI
                     EALEDYFGIPYPYRKLDLVAVPDFAAGAMENAGLITFREWLLLIDEAHATESQYRAFA
                     YVMAHELSHQWFGNLVTMPWWDDIWLNEAFATWMGNKVVQALHPEYRSDLAALGSGQR
                     AMDLDSLTSARSIRQPIESNHDIKNAFDAITYEKGGSMLSMFERWMGVDVFRDGIRLY
                     LRRHENSTATSDDLLAALDEASDLEVTAPFLSFLTRPGVPLMTVAGDEGCEGGFRNLR
                     LSQQRYLPIGSRGSPKQTWKIPFCVQFDLGVSCGLLTEAEASVQVPGCPSWWMPNADG
                     AGYFRFAMSPDEWTHLRTEGFAKLSDGGQMAVADSLAAAFDRGALGAEDLLPWFPIFV
                     RSPLRQIATAPMESLRFMIVQGAPPDLRGEVTKYASRLYRSRYERLGWRTRNGESSDT
                     KLLREAVIRFMVMDVRDGGARARAAKLGRTYVGYRTQAKPSVVDAQLADLVLAAAVQE
                     GGEALFDHLLHLLASSTDATTRNRIISALGHAEAPSLSERALDLALDPGLRVNEIPRL
                     LGTQFGNPRNRERAWEWLTEHFDALAARFGSTQVGGTPWYASSFCSETAADDVARFFE
                     PRVSELDGGPRNLAGAVEAISLCAEKTRVQQPGVERAFAGK"
     gene            complement(9632..10702)
                     /locus_tag="VFG22_08380"
     CDS             complement(9632..10702)
                     /locus_tag="VFG22_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416300.1"
                     /translation="MISLLLLAASAVVPWLVCSILRGIFGSDESGSIPARERALGRYR
                     RATLVSGLLVLPAAAVAGALLVDPSLSTRSPAAGSWFFASLSATTAWVSLALARRTRE
                     EAAAMSALQTMGRAVQTASVLILATGLALLAGSAADLLLPSGRIAAALTVALLSVVSV
                     LVLSPWLVIMLGLWPVLPKRIEAGGRQWKLAHLPAPTRFLTHTAALPWLRTVLVSDGL
                     FNGAPERHWENLVRYEIGGALHARSARSSRWTVAVVLSLMAFMVAAALSANDPAMRVA
                     TTALAVLFTSTVAWLANRQPSSTLQFDSDGPSMQELAQTLRSLPPRLGQALPRTSCNP
                     LGSALYDRLFALGHDPGRRPQP"
     gene            complement(10699..11469)
                     /gene="xth"
                     /locus_tag="VFG22_08385"
     CDS             complement(10699..11469)
                     /gene="xth"
                     /locus_tag="VFG22_08385"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HET6416301.1"
                     /translation="MKVATWNVNSIRARRQHVLDWLDASEPDVLCLQELKVTDADFPY
                     GELQERGYEAAVFGQKTYNGVAILSRHPIENVVEGIDDDEDDPQARLISGDVGGVTIL
                     SAYFPNGSEPGSDKFEYKLRWMSRLRALLGRRFDPENDQVALCGDFNVAPFDDDIGRP
                     AEWRSSVLACDPVRDALNEVASFGLHDLFRPFHPAGGVFSWWDYRGRGFERNNGLRID
                     HVYGTRKLAERTLGAIVDREERGRKSPSDHAPVMVELA"
     gene            11494..11886
                     /locus_tag="VFG22_08390"
     CDS             11494..11886
                     /locus_tag="VFG22_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET6416302.1"
                     /translation="MTNKRIETDRAPAAIGPYCQAVAAGEWIYCSGQLPLDPDTAEMV
                     GDGDIKAETLQVLQNLDAVLRKAGASRADVVKATVYLTDMNEFSNMNQIYAAFFKDVV
                     PPARATVQVAALPRGSKIEIDFIARLDD"
     gene            complement(11890..13170)
                     /locus_tag="VFG22_08395"
     CDS             complement(11890..13170)
                     /locus_tag="VFG22_08395"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HET6416303.1"
                     /translation="MSALVVVGAQWGDEGKGKVVDLYAPYAELVVRYAGGANAGHTLV
                     VNGEKLILHLIPSGILHEETKCVVGQGTVIDPAVLLKEIRSLDERGVATQGHLLVSQR
                     AHVVLPHHKSIDELREKRSGGAIGTTKRGIGPCYEDKVARRGVRMGDLLNPGRLRAKL
                     ESNLECWRPVALDLGGTLPTADEITTAYSELGQALSAYIGDAAAIIRAAVQADEKILL
                     EGAQGTMLDIDNGTYPFVTSSNAVAGGACAGAGIGPTHIRRAIGIAKAYTTRVGGGPF
                     PTELDDETGQRLRDAGAEYGSTTGRPRRCGWLDLVALRHAVAVNGLDEIAITKLDVLS
                     AVPELKICVAYDLDGKRLSHPPYERVEDVVPVYETLEGWSEDLSGCRSRGDLPAAAQA
                     YLSRIEKEVGCRVGVVSVGPDREDTADLSDPFDR"
     gene            complement(13167..14510)
                     /gene="hisZ"
                     /locus_tag="VFG22_08400"
     CDS             complement(13167..14510)
                     /gene="hisZ"
                     /locus_tag="VFG22_08400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HET6416304.1"
                     /translation="MPPSARKRPPGSAARLGITNAGLPLTPPGGMRDLLPPASMTRTL
                     LWQQLMETFDLYGYQRVTTPPVEYAEVIERGLGTVDRRDLVRFVEPDSGEVALLRPDI
                     TPQIARIVATRLQDRPPPWRLCYEGTIIRRRRGRARKQHQVLQCGIECVGLKGPEADA
                     EVIEVAARACERVGLRDFLVELAQVKIGAEVLEHVPANARLAVIETLAAKDATNLEKL
                     LKESRVTAAERRRLLMLCDLYGDRTVIADARRGLQNAAAKRALDELERVVDRLGAVGL
                     GERVAIDLSDLRRHSYYTGVSMTLLAAGPGEPLGMGGRYDDLLGRYGAPSPATGFAFE
                     VDNVLWALSEAGVSFQGKLPLRVVLAGGSYARSARFAGAWRARGARAAVIPAKSADEA
                     KRYAKAWDYDLLVWQPGSGAQLTRLKDGRTRTLKGQPSDESFSELVSWARVGNES"
     gene            complement(14512..16101)
                     /gene="serA"
                     /locus_tag="VFG22_08405"
     CDS             complement(14512..16101)
                     /gene="serA"
                     /locus_tag="VFG22_08405"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HET6416305.1"
                     /translation="MSNVRVLVSDKLAEAGLRVLREAPGVELEFRPGMSEDELCSVIG
                     EYDGLIIRSATRVTPRVIEEAHRLRVVGRAGIGVDNVDIPAASRRGIVVMNTPTGNSV
                     TTAEHALALLASLARNIPQAVASMRDGNWEKTKFQGREIAYKTLGIIGLGNIGRIVAD
                     RAQGLKMKVVGVDPVMSSDRAAELGIELVELDDLLARADFITIHAPLTPETKNLFSDA
                     AFEKMKKDALLVNAARGGIVDEEALVRAITGGKIAGAALDVFGTEPIDPKHPLLGLDR
                     VLCTPHLGASTAEAQERVAVEIAEQAIAYLQLGIVKNAVNVPALPQETAERLKPYLDV
                     ARALGSLVGQLDPIDVRELRVTCTGEAGELGVTPIARAALAGFLEQHLEEPVNPISAP
                     YEAQERGIRLAEVKEPSDRYSTTVRVTVTGEKGIHTATGTPGRKGEPRLVGMEGYEID
                     AVMEGSILIMRNQDRPGVIGSVGTLLGSRGINVSRMQVGLDEKSGQALALWNVDSNVG
                     KDALDELRAIDYVSSVHTLRL"
     gene            complement(16189..18186)
                     /locus_tag="VFG22_08410"
     CDS             complement(16189..18186)
                     /locus_tag="VFG22_08410"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6416306.1"
                     /translation="MPRPTSPPPAPPPNTVLGAYTLVDRIAVGGMAEVFRALEPRGAG
                     EPRVVVLKRMLPHIANESNAQQMFAEEARLASQVTHPNVVHVLGHGEIDGKPYLTLEY
                     VPGCDLWRLNRRLTLESKKLEHDLIVYLIRQILSGLEAVHDAKDRSGSTLGIVHRDIS
                     PSNVLISIHGDVKIADFGIASANVNALEADTAARANGKLGYLAPEQVSGGAVDRRADL
                     FSTGVIAAELFMRRPLFAGGSELAVLLAIRDAEVHPFIEFIPQLPVGLGEVILSALAA
                     RAEERPPTARAFWAALEPWQSRPEEVLRAELAAIVGSIAAETRMRRTSADTLPKATMP
                     PTPLVLAGDRANTTNVTPLEYKIRKPNGATVGPLAYAKVVEAVATGQVDGSDDISIAG
                     GDFQPLSSVKDLYRHVPASRMTPTTTRVESTASADRSMDFEEGGFVSALVRTLVTRRT
                     GLWLCEQGGVRKEVYVKHGVPAFVTSNLAGELLGEYLVKKRVINRSELDMALAVMPRF
                     EGKLGDTLVALGLVEPLELFQHIGEQVREKLLDLFTWASGTATFYEGVDPPSSSFPLR
                     LDAWEILDRGLQRRIAAGFESALILERGQDLLVQADRIDTAVATAALPDDLQAALKRC
                     TTPQTVEQLAQFVDARSGHTDPQRGYRVASLLLSLGAIHWM"
     gene            18261..18926
                     /locus_tag="VFG22_08415"
     CDS             18261..18926
                     /locus_tag="VFG22_08415"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6416307.1"
                     /translation="MADPSSRAGERYASAQILEYAARTHHPADPALLRAFEAPARHGM
                     PEIQLGPAEGRLLELLLRLAGAKRVVEIGTLAGYSALWIARALPPGGHLWTIEADRKH
                     AGVAAEVIGEAGLGDRVTVIADEALAVLPKLSDSGPFCAVFLDADKGRYDIYGRWATE
                     NLRPGGLLIGDNAYLFGSLLEQSEDAQAMRRFHEEMASNYRSVCVPTPDGLAVGIRLD
                     SKA"
     gene            19161..19799
                     /locus_tag="VFG22_08420"
     CDS             19161..19799
                     /locus_tag="VFG22_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HET6416308.1"
                     /translation="MQIFPRELNYLPLALAVVAGGLGAGVFILFWVYFTPPNLQVGYT
                     PKQPVAYSHRLHAGELGMDCRYCHSQVERSAKAMVPPTQTCMGCHAVVRTESEKLALV
                     RDSWATDESIPWVRVHQLPDHAFFNHSAHLNGGIGCQSCHGRIDQMEVVGVVSPIAMQ
                     WCLECHRNPEPHVRPKTEVTNMKWEADEAWLAQRAEVAATLNPPLHCSGCHR"
     gene            19796..>20304
                     /locus_tag="VFG22_08425"
     CDS             19796..>20304
                     /locus_tag="VFG22_08425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HET6416309.1"
                     /translation="MTRRNSYELNPGKGPSMWRTLEDKNTDPEELKKLAEEEQPGGFI
                     TDLLSPKALISRRSFVQGSSIAALAAGVQGCVRRPVNEILPYSKAPEYLVPGIASHFA
                     TVTARGRDSLGVVVTSHDGRPTKVEGNPEHSRSFGGTDVRAQAYVWDLYDPDRSQSPA
                     QRKGNALVNA"
BASE COUNT         3698 a         6341 c         6612 g         3653 t
ORIGIN      
        1 ggtcgcatcg caggtcggcg gctcctcggc gacaccctgc aatatgcgcg tggcacctgg
       61 tacaactccg cgaagctcat ggacatcgaa tacacgactg ccggcctggt gaactggaaa
      121 ctcgagcggg aagagaattg gttccacgaa gagcggggca aggtcaacag cacgacgcgg
      181 atcgtggcgc aggatgatcg ggtcgtgggc ttcaacatgc tgggtcgccg ctgggatcac
      241 agtgtgctcg cgcggtggat cgaggaacgg cgcaagctcg attgggtcct ccagcacttg
      301 aacgatgcag ccttcgacac cgagttcgtc gcgccgctcc gacttccgag gagatgacgt
      361 ggatcagcaa ggacaaaacg ggctactggc gacctatcga agcggctggc gcaaccgcag
      421 cccgatcggc attgcgatct ccgtgctctt ggtgagcttc tacgtcgttc tcttctggga
      481 gcacaaactc caggcggcgg ccggtgttgc gccgttcacc gcgttctccg aggcgcttgg
      541 cttgcgcaat cgctggtacc tctacggatt cctgtactcg atcgccatga gcgctggagc
      601 cgtgttctac cttcggcgcc acggcaacag ccgctacaac cgttttcgaa tcacggtgaa
      661 cgtggcgatt caaatcatgc tcggtttcac gttgccgttc atcatgcccc tctttggcgg
      721 caaggagctc tacttctcgt acttttggcc gctcaagtac gactacttct atccgcaaac
      781 cttggccgat gtgccactgt acttcgccct ctacaccgtg ctgggttccc tcatcgccgc
      841 ccccattctg gcggtcttct acgggaagcg ctggtactgc tcctgggtct gtggctgcgg
      901 aggcttggcg aacaccttcg gtgacccgtg gagacacctc acgagcaagt ccacgaagtc
      961 ttggcgcttc gagcagctga gcattcatct cgttctcgcc cttgcagtgg cgacgacgtt
     1021 tctgatcgga ctcgattcgc tcatcggcag cgagcatccg accttccacc gaatcgtggc
     1081 gggcgaggat tggcaggatt ggtcggtcaa gagcttctac ggatttgcag tcggcgcggt
     1141 actggctgga gtcatcggca caggcctcta tccagttctc gggccacgcg tctggtgccg
     1201 gaatttctgc ccgatggcgg cggtgctcgg atggatccag aaggtggggc gctatcgcat
     1261 caaggtgaag cccgacatgt gcatctcttg tggcaattgc agcacctact gcgaaatggg
     1321 gatcgatgtg cgctcgtacg cccagaccaa ccagagcttc acccgcgcgt cctgcgtggg
     1381 ttgcggcatg tgcgcacaca tgtgcccacg aggcgtgctg aagctcgaga gctcatggca
     1441 gcgggccgaa gacaagcagc gggagaagct ctcggtcgtc gatttctgag gctcaggcga
     1501 acgcgcggtt ccaggcggcg aggatctcgg ccgagtacag aacgaagcga atctccctga
     1561 ggtcccattg cttttcgcgt acgaccttcg ccgaaacggc agccgcctcg tcgatcgggt
     1621 agccataaac gccgcaggag atggcgggaa atgcgatcga ttccagacca ttttcgactg
     1681 caagtgcaag cgacgatcga tgcgccgccg agagcaagtc agggtcctct gcgcgccctg
     1741 cgtacaccgg gccgacggta tggatgacat accgggcccg aaggtcgaag ccgggcgtga
     1801 tacgtgcctc gcccctgggg cagcgcaccc ccggccgcag ctccggaagg gcacggcagg
     1861 cgcgaaggag cgcggggccc gcagcacgat ggatggctcc gtccactccg ccgccgccga
     1921 gcagccttgg gttggccgcg ttcacgatcg catcggtgtc cgcatggcag aggtcgccct
     1981 ggacgatgcg aaccttggtg ctcatcacgt tttgtcgacc ggcgcgggct tgcccatgag
     2041 ctgctcgaag gcaagaggtc cgagcgattg aaccatcagt cggtccaagc gccgctcgat
     2101 cgcctccttt tgctggggcg agtcgtagag gaacgcgatg cccatgccgg ctggacgtct
     2161 ggactcggct tttccggcct cgacgaccca gcgcacggcc ccccggagca cgagcggttc
     2221 ccctagctcc ggggctcgaa tggtgaacga gagcacagtt ccaaggggta aggggcgatc
     2281 ggttcggatg aaggttcccc cccggctgat gttgtttgtg taatcggaca ggagcgcgtt
     2341 cagacggtcg tattcgatgg gcaattcgag cgttgcccgg cctgactctc gacgctctcg
     2401 gcccccttca gacatcgtct gccgcccagg gtagagtccc tccgagcccg cggcaacaac
     2461 acggtcgctt gacgccgaat cgggcactgc ctaatctacg agacccttct aacccggacg
     2521 ttccgcagtg gcgttgagcg tctttcgcgc gcgaggattg cgcatgactg aaaccgattc
     2581 caaaacaagt gagacctcaa aggaggaggt ggcgcctgtg gaatcgcaag ccaccgtttc
     2641 ctcctatcaa cctcaggtcg aggctgtgcc tgtcgctgcg acggtgacgg tcacggagcc
     2701 tgcgcccgag cctgctgaga agacggcgga agccgacaca gtggcaggcg aggtgactgc
     2761 agtggagaca catgcggaca ccgtcagcgg cgaatcggct ccggagacgg gcgaggagag
     2821 ctccgcgggc tctgtcgcgg ccgaaggggc gggcaagaag aagcgtcggc ggcgcaggaa
     2881 gaagggcgga caagcggggg cgagtgagtc gccgtccgat ccgaagtcgg ctcatcatca
     2941 tgcgccgttc ctgcacttgt tctcgggcgg cgcggcgcgc aagcacgcct tctcggcggg
     3001 cgaggagatc gctgggcgtg tggagcgcgt ggagcacggc accgtcatca tcgacgtgtt
     3061 cggcaaagcg accgccatcg cgaacgtcga cgagccacga gacatcgcgc acgccgagcc
     3121 cgtggtcgac ccaatcttgg cgataaccca ggccagcgag gctacggagc ttcccgaggc
     3181 gcacgttccg gttcacacgc cagtggttcc cgcgattacg acgactccga cgcctgtcgt
     3241 ggaagcgccg accccggaga tggccaagct ggcacagggc gacgcatcga gctgggagtt
     3301 tcagcccacc gaagtgacgg acgtgcccga cgatgagctc ggcgaagccg atacggtcgt
     3361 cggtgcgcag gcgttcgacc caggcagctt gatcgacccg cagcttgcgg ccacacagcc
     3421 accggcgatc gcggtgacgg agccagtgcc tgcggcctcg tcgaccgaag aggttgcgcc
     3481 agagtccgaa gcgatgagcg agcctcctga ggacccagcg gaggcggatc tgagcctcct
     3541 cgatcctctg ccagagttcg aagctccgca gattggcgcg atcttccgcg gacgcatcgg
     3601 cggtgtagcc gagagtggtc acatcgtgat cgtcaatcgg ctgatcgaca aggccgcggc
     3661 ccgcaaggcg ctgcgtgcgg cgcgtgatct ccacaagcgg gtcgaaggcg tcgtgtacgg
     3721 cttcaaccgc ggtggcttcg atgtgctcgt gggtggcgtg cgcgcattct gccccgcaag
     3781 cgcgatgtcg ctcgcgccca tcgatgatcc gaacgcgttg gtcggacacc ggttcgagtt
     3841 ctctcttccc ccggacaagg gcggcaacaa gaagagcatc atcgtcagcc ggcgaggcat
     3901 tctcgagaaa gaagctcgga cgaaggctcg cgaacggatg gcagagcttc aggtcggcgc
     3961 gaagctcagg ggcaaggtcc tcgaggtgcg cgactacggt gtgctcgtcg atttgggcga
     4021 cggcctcgat ggcctggtcc atatgagcga ggtggcctgg ggccggggtg tgcgtccaca
     4081 ggacgcggcc aaggtcggcg acgaggtcga cgtcgaagtc ctgaaggtcc aaccggcctc
     4141 gcgtaaggat cggttcgggc gcgtgtccct ctcgatgcgt gcctgccaac ccgacccgct
     4201 ggacgccgcg aaggattcga tccggccggg aatgcccatc aagggcaagg tcgttcggac
     4261 caccgagttt ggcgcattca tcgagctcca ggaaaacatc gaagggttgc tgcacatctc
     4321 ggagctcggc ggaagcagag agctgacgca cgccaagcag gcggtcaccg agggcgacga
     4381 gatccacgtc gtcgtcgagc gcgtggatcg tcagcaacgc cggatctcgc tctcgaaact
     4441 ttcggaagcg gacgctgccg cgatcgaggc aggcgagatc gaaatcggca ctgcacctcg
     4501 ttctctcaag ccgggaactg tggtttcggt gatcgtcaag cgcgtcgatc acagcggcgc
     4561 gcaggttcag gttggaggga tgcttggcaa gcgagggcgc ggattcattc cgaaccgtga
     4621 gctgcaggat gtccaggaag acaagcgcaa gggcgtgtcc cagggtgacg tcatcgaggt
     4681 gaaggtcatc ggaaccgagc gcgacggcac gctgcgttgc tcgatccggg cccgccttct
     4741 cgacgacgag cgaaaggccg tgcgtgagta ccgcaaggag tccgcgaaac agggcctcgg
     4801 caccttcggg gacttgctga aggcgaagct cggaggctcc agcgagtaga tcgcggcgca
     4861 gcgtccctca atcgcgaatt caccggctac gccggcttgt cgcttggctg ttcgtcggat
     4921 aggctccgcg ccgttctgaa ctgggcgatt aactcagggg tagagtggtt tcttcacacg
     4981 gaacaagtcg ctggttcaaa tccagcatcg cccacccatt gctccagttg ccttgaaggc
     5041 ggccctttga agccgcccga aggcagctag agccaatcat aggagaatcc cttggctgac
     5101 ctgtcgactt tggtccagcg aattcagtcc ggggagatgc gcgcgctcgc tcgcgcgtgc
     5161 cggctgatcg acgagcggga tccgcgcgcg tttgatttgc tcaagcaggt ctttacccag
     5221 gacgggcacg cttacgtgat cggcgtcacg ggaacaccgg gcgccggaaa gagcacgctg
     5281 gtcgacggct tgattcgact ctaccgccag caagacaagc gtgtcgccgt gctcgcgatc
     5341 gacccgacga gcccgtattc gggcggcgcg attctaggcg accggagtcg catgcagcag
     5401 cacttcaccg aagagggcgt gttcattcga tcgatcgcca ccagagggca cctcggcggg
     5461 ctgtcgcgct cgactggcga cgtgcttcat gtcctgcaag ccgcgcaatt cgatgtcgtg
     5521 attctcgaga ccgtaggcgt tgggcaggac gagctcgaag tcacgggtct cgcggacacg
     5581 acgctggtgg tgatggcgcc tggcatgggc gacgacgttc aagcgatcaa ggctggcatc
     5641 ctcgaagtcg ccgatgtctt tgcggtcaac aaggccgatc gtgtcggcgc agacgccacc
     5701 gctcaggacc tccagcagat gctttcgctt cgccgggtcc ccgtgggggc gagcaaaatg
     5761 caggggcaca ccgcggtttc cagcttgcgg aagggtccgg aggccaaagc agacgagtgg
     5821 gtcccaccga ttctcaagac ggtggcgcac cgtagagaag gcctcgaaga gctgatcgac
     5881 gcttttgcca ggcatcggga gttcctcgcg accacggaga ccggtcgaag gctccgcgag
     5941 cagcggcgca tcgccgagtt tcgggccatc ctctaccagg cactgatcga cgaagtggat
     6001 ctgcgcttcg cggaccgggt gacggagcgt ctgcaggaca ttctcgatgg acgggcggac
     6061 ccgtattcga gcgcagaggc gctcgtgcgc ggcgcgctcg gagacgacat ctaggagcct
     6121 ctggcaacgc ggttcccttg ggctagagtg cgcgacatga gctcgtttgt ggaagtgacg
     6181 cacgaaggcc acgtcagtac gttgacgatc aaccggccgg acaaactcaa cgcgctgagc
     6241 cgggaagtgc tttcggacct aggtgccgct atcgacggcc ttgcaggcaa tcaggctgtt
     6301 cgagctgccg ttcttaccgg aacgggaaaa gctttcgtcg caggcgccga catcgcggcg
     6361 atgaagaata tgaccgaagc cgagggacgt gcgttcggcg cgcttggaca cggggtattc
     6421 gcggcaatcg agcatctgcg ctttcccgtg atcgcggcgg tcaacggctt tgcgctcggc
     6481 ggcggctgcg agctcgccct ggcttgcgat ttcatctacg cgtcgagcaa agcgaagttc
     6541 ggacagccgg aggtgaacct cgggatcatt cccggcttcg gcggcacgca gcgtcttccg
     6601 cgtcgggtcg gacccgcgcg cgcgcgtgag ctgatctaca cgggaaagat gatcgacgcc
     6661 gcggaagcgc tccgaatcgg tttggccaac gcagtattcg aaccggacga gttgctcgcc
     6721 ggagcgcaga aggccgcttt ggagatcgcg gccaagggac cgctggcggt ctccgcggcg
     6781 aagcgcctga ttcgcgatgg aatcgatctt ccgttaccgg ctgccaatca gctcgaacag
     6841 aatgcgttcg gagaagcgtt cggcaccgag gatcagagcg aagggatggc cgcgtttctc
     6901 gagaagcgag cgcctacgtt caagggagtc tagagctcgt cctacttgcc cgcgaacgcg
     6961 cgctccacac ctggctgttg gacgcgtgtt ttctcggcgc acagagaaat cgcctcgacg
     7021 gcgccggcaa gattgcgcgg tcctccgtcg agctcagaca cgcgcggctc gaagaaccgc
     7081 gccacgtcgt cggcagcggt ttcgctgcaa aaggacgagg cataccaggg cgtgccccct
     7141 acctgagtgc ttccaaaccg ggccgcgagg gcatcgaagt gctcggtgag ccattcccag
     7201 gcgcgctcgc gatttcgagg gttgccgaac tgcgttccga gtaagcgggg gatctcgttt
     7261 acgcgaagac cagggtcgag cgcgaggtcg agagcccttt cggagaggga cggtgcctcc
     7321 gcatgaccga gagccgagat gattcggttg cgggtcgtcg cgtcggtgct cgacgcgagg
     7381 agatggagaa gatggtcgaa gagagcttcg ccgccttctt gcacggctgc tgcgaggacc
     7441 aggtcggcga gctgagcgtc gacgacactg ggcttcgcct gggttcgata accgacgtag
     7501 gtccggccga gcttcgccgc ccgagcgcgg gctcccccgt cgcgcacgtc catcaccatg
     7561 aagcgaatga cggcttcccg aagaagcttg gtgtcgctcg attcgccgtt tcgggtccgc
     7621 catccgagtc gctcgtagcg actacgatag agacggctgg cgtacttggt gacttcgcct
     7681 cggaggtcgg gcggcgcgcc ctgcacgatc atgaagcgta gggactccat cggcgcagtg
     7741 gcgatctgtc tgagcgggct ccgaacgaag atcggaaacc agggcaggag atcctccgct
     7801 ccgagcgccc cgcggtcgaa cgcggcggcc aagctgtcgg ccacggccat ctgaccgcca
     7861 tcggagagct tggcaaagcc ttcggttcgc agatgcgtcc attcatctgg ggacatggcg
     7921 aaacgaaaat aaccggcgcc gtctgcgttg ggcatccacc aggacgggca accgggaacc
     7981 tgaacgctcg cctccgcttc ggtcagcaat ccacagctca ctcccaggtc gaactgaaca
     8041 cagaagggga tcttccaggt ttgcttcggg ctgccgcgcg agccgatcgg gagatatcgc
     8101 tgctgcgaga gtcggaggtt tcgaaagccg ccctcgcagc cctcgtcgcc ggcgaccgtc
     8161 atcagcggaa ccccgggtct agtgaggaag ctgagaaacg gtgccgtcac ttcgagatcg
     8221 ctcgcctcgt cgagggcggc gagcaggtcg tccgaggtcg cggtgctgtt ctcgtggcgt
     8281 cgcaggtaga gacgaatgcc atcgcgaaat acgtcaaccc ccatccagcg ctcgaacatc
     8341 gacagcatcg agccgccttt ttcgtacgtg atggcgtcga aagcgttctt gatgtcgtga
     8401 ttgctctcga tcggttggcg gatgctgcgt gcgctggtga ggctgtcgag gtccattgcg
     8461 cgctgcccag acccgagagc agcgagatcc gagcgatact cggggtgtag cgcttggacg
     8521 actttgttgc ccatccaggt ggcgaaggcc tcgttgagcc aaatgtcgtc ccaccaaggc
     8581 atcgtgacga ggttcccgaa ccactggtgg gagagctcgt gcgccatcac gtatgcgaat
     8641 gcgcgatact ggctctccgt cgcgtgtgcc tcgtcgatca atagaagcca ttcgcggaac
     8701 gtgatcaggc cggcgttctc catcgcgccg gccgcgaagt cgggcacggc gaccaggtcg
     8761 agcttgcgat acgggtacgg gatgccgaaa tagtcctcca gcgcctcgat gaacttcccg
     8821 gttcgctgaa gagcgaaccg aagctgctcc cctttgccct tggccgccag cccgcggagc
     8881 gggatgggaa agcttcgctc cttcgtgggc gcgatggtcg gcccgatgac gacgtcgagc
     8941 ggaccgacgg cccaagcgac gaggtaagtg ggcagcggtg gcgtaggcag aaagctgacg
     9001 cgttgaaggc cattgggaag cctagtcgca cgttcgatcg gggtgttcgc ggcggcgatc
     9061 tgatcgtcgg gaaccgtgag ggtcacttcg aagggggtct tgaaacgcgg ctcgtcgaag
     9121 caggggaacg ccagccgtgc gctaatcgac tcgaactgag taaatgcgta cgaatcgtcg
     9181 ccggactcga cacggtagag tcctcggagg ggcgtatcga actttgcgct gtactcgatc
     9241 tcgagagtgc tccggccctg gggaagtgta tccgtgaaaa ccacgcggac caccccatcg
     9301 ctcgtttgct gctcccaatc cgcttgcaca cgcgcgctcc catgtcgtgc gtgtatcgac
     9361 gtcacgtcca agtcctggcc gtgcatccag atgatgctcg atggggcgtc gagctcgacg
     9421 tcgatgtccg cagtgccgtg gaatgaatcc ccatcgggga tgatctggag gttcaagcga
     9481 taggccgtcg gccgtacccc atcggggagc ttgccctcgg gcgctcgcac gggcatgggc
     9541 gtcggctcga ctgccgtctt cgagccgccg caggcgatca gagcggcgca gaacgcaagc
     9601 aagggaaaat gcactcgttc caagattcgg ctcacggttg tggccttcgt ccggggtcat
     9661 gtccgagcgc gaacagtcga tcgtaaagag ccgagcccag aggattgcac gaggtgcggg
     9721 gaagcgcttg gccgagacga gggggcaagc tgcgcagcgt ttgggcgagc tcctgcatcg
     9781 aggggccatc cgagtcgaac tgcagtgtcg aggatggttg tcgattggcg agccaagcca
     9841 ccgtgctcgt gaagaggacc gctaacgccg ttgtagccac ccgcatggcc gggtcgtttg
     9901 cgctcagcgc cgccgcgacc atgaacgcca tgagcgacaa aacgacggcg accgtccagc
     9961 gagacgaacg cgcgctccgc gcgtgaaggg cgcccccgat ctcgtagcgg accagatttt
    10021 cccaatgcct ttcaggggca ccgttgaaga gaccgtcgct caccaatacc gtgcggagcc
    10081 agggcaaggc agccgtgtgc gtcagaaacc gggttggcgc cgggaggtgg gcgagcttcc
    10141 attgcctacc gcccgcttcg atgcgcttcg gcagcacggg ccagagcccg agcatgatca
    10201 cgagccatgg gctcagcacg agaacagaaa ccaccgaaag cagggcaacc gtcagcgcgg
    10261 ctgcgatgcg ccccgaggga agaagcaagt cggccgctga gccagcgagc agtgcaagcc
    10321 cggtagcgag aatcaggacc gaggcggttt gcaccgctcg ccccatcgtc tgtagagcgg
    10381 acatcgctgc cgcttcttcg cgggtccgcc gggcaagagc gaggctgacc caggcagtgg
    10441 ttgcgctgag gcttgcgaaa aaccaggagc ctgcggccgg cgatcgagtc gacagcgacg
    10501 ggtccacgag tagcgctccc gcaactgcag cggccggaag gacgaggagg cccgaaacga
    10561 gtgtggcgcg gcgatagcgc ccgagagcgc gttcccgggc aggtatggat cctgactcat
    10621 cgctgccaaa aatcccccgc aggattgagc agaccagcca tggaacgacg gctgatgcag
    10681 ccaagagcag gagcgagatc acgcgagctc caccatgacc ggcgcgtgat cggagggaga
    10741 tttccgtccg cgctcctcgc gatcgacgat cgcgcccagc gtgcgttcgg ctagctttcg
    10801 cgtcccatag acgtggtcga tccgaagccc attgttgcgc tcgaacccac gcccgcggta
    10861 gtcccaccag gagaagactc cgcccgccgg atgaaatggg cggaacaggt catgcagccc
    10921 aaacgaagct acctcattca aggcgtctcg aacgggatcg caggcaagca ccgatgagcg
    10981 ccattccgcc ggacgcccga tgtcgtcatc aaagggagcc acgttgaagt ccccgcagag
    11041 cgcgacctgg tcgttctccg gatcgaaacg cctgcccagg agcgcacgga gacgcgacat
    11101 ccatcggagc ttgtactcga atttgtccga acccggctcg ctgccgttcg ggaagtacgc
    11161 actgagaatc gtcacgcctc cgacatcgcc gctgatgagc cgggcctgtg ggtcatcctc
    11221 atcgtcgtcg atgccttcca ccacgttctc gatcgggtga cggctcagga tggcgacgcc
    11281 attgtacgtc ttctgcccga acacggccgc ctcgtatccg cgttcctgga gctcgccgta
    11341 cgggaagtcc gcgtcggtca ccttgagctc ctgcagacaa agcacatcgg gctcggaggc
    11401 atcgagccag tcgagcacgt gctggcggcg cgcccggatc gagttcacgt tccacgtggc
    11461 gaccttcatc ccaggatgaa gtagcatgga atcatgacga ataaacgaat cgaaaccgat
    11521 cgcgccccgg cggcgatcgg accgtattgc caggcggtcg ccgcgggtga atggatctat
    11581 tgttccggtc agctaccgct cgatcccgac acggctgaga tggttgggga cggcgacatc
    11641 aaagccgaga cccttcaggt ccttcagaac ctggacgccg ttcttcgcaa agcgggcgcg
    11701 agcagggcgg atgtcgtgaa agcgacggtc tatctgacgg atatgaacga attctcgaat
    11761 atgaatcaga tttacgcagc ctttttcaag gatgtcgtgc cgccggcacg cgccaccgtc
    11821 caggtggccg ctcttccgcg aggctcaaag atagaaatcg acttcatcgc caggctggat
    11881 gactgagctt cagcggtcga acgggtcgct gaggtcggcc gtgtcttctc ggtcggggcc
    11941 tacgctgacc acacccactc ggcagcccac ttctttctcg attcgactga gataagcctg
    12001 ggcagcggcg ggaagatcgc ctcggcttcg gcagcccgag agatcctcgc tccagccttc
    12061 caaggtttcg tagaccggga ccacgtcctc gacccgctcg tagggtgggt gactgagacg
    12121 tttgccatcg aggtcgtagg cgacgcagat tttgagctcc gggacggcgc tcagcacgtc
    12181 gagcttggtg atcgcgatct cgtcgaggcc gttgacggcc accgcatgcc gtagcgcgac
    12241 gaggtcgagc cagccgcagc ggcgcggccg cccagtcgtg gagccatact cggcgccggc
    12301 gtcgcgaagc cgttgaccgg tttcgtcgtc gagctcggtc gggaaggggc caccgccgac
    12361 gcgcgtggtg taggcctttg cgattccgat cgcccgccga atgtgggttg gaccaatccc
    12421 tgcgccagca caggcgccgc ccgccaccgc gttcgagctc gtgacgaacg gatacgtgcc
    12481 gttgtcgatg tcgagcatcg tgccttgcgc gccttcgaga aggatctttt catccgcttg
    12541 gactgctgcg cgaatgatgg cggccgcgtc tccaatgtat gcgctcaagg cttggccgag
    12601 ctccgagtac gccgttgtga tttcgtccgc agtcggcaac gtgcctccga ggtctagtgc
    12661 caccggacgc cagcactcga ggttcgattc gagctttgcg cggagccgcc ccgggttcaa
    12721 gaggtctccc atacgcacgc cccgccgagc taccttgtcc tcgtagcagg gcccgatgcc
    12781 gcgcttggtc gttccgattg caccgccgga gcgcttctcg cgaagctcgt cgatcgactt
    12841 gtggtgcggg agaacgacgt gagcccgctg actcaccagg aggtgaccct gcgtcgccac
    12901 gccgcgctcg tcgagggacc ggatctcttt caacaagacg gccgggtcga tgaccgtgcc
    12961 ctggccgacg acgcacttgg tttcttcatg gagaattccc gaggggatca agtggaggat
    13021 caatttctcc ccgttcacga cgagcgtgtg ccccgcgttt gcgccccccg cgtatcggac
    13081 caccagctcg gcatagggag cgtagaggtc gacgactttg cccttgcctt catcgcccca
    13141 ctgggctcct acaacgacca atgcactcat gattcgttcc caactcgtgc ccaagagacc
    13201 aactcgctga acgactcgtc gctgggctga cccttgagcg tccgagtccg accgtccttc
    13261 agacgcgtca gttgcgcgcc gctccctggt tgccagacca gcagatcgta gtcccatgcc
    13321 ttggcgtagc gtttggcttc gtcggcgctc ttggcgggaa tcaccgcagc acgtgcaccg
    13381 cgtgctcgcc aagcgccggc gaagcgcgcg cttcgcgcat agctgccgcc cgccagaacg
    13441 actcgcaagg gcagcttgcc ttggaacgag acaccggctt cagagagggc ccagagcacg
    13501 ttgtcgactt cgaacgcaaa gccggtggca ggcgaaggcg cgccatagcg gcccaagagg
    13561 tcgtcgtatc tgccacccat gccgagcggc tcgcctggtc ccgctgcgag caaggtcatg
    13621 ctcacgccgg tgtaatagct gtgacggcgc aggtcgctga ggtcgatggc aacgcgctcc
    13681 ccgagcccta ccgctccaag tctatccacc acgcgttcga gctcgtcgag cgcgcgcttc
    13741 gcagccgcat tctggagccc tcgtcgcgca tcggcgatca ccgtccggtc tccatagagg
    13801 tcgcaaagca tgagcagacg ccggcgctcc gccgcggtca cgcgcgactc tttgagcagc
    13861 ttctcgagat tggtcgcatc cttcgccgcc agtgtttcga tcaccgcgag gcgggcgttg
    13921 gcgggcacgt gctcgagaac ttcggcgccg atcttcacct gcgcaagctc caccaggaaa
    13981 tctcgaagcc cgacacgctc acatgcgcgt gcggcgacct cgatgacctc cgcatctgct
    14041 tcgggcccct tcagacctac gcactcgatt ccgcattgca gaacttgatg ttgcttacgc
    14101 gcccgaccac gtcggcgccg gatgatcgta ccctcatagc agaggcgcca ggggggaggg
    14161 cggtcctgaa gtctcgtcgc gacgatgcgc gcgatctgcg gcgtgatgtc gggtcgcaga
    14221 agggcgacct cgcctgagtc gggctccaca aaacgaacca agtcgcggcg gtcgacggtg
    14281 ccgagccctc gttcgatcac ttcggcgtac tcgaccggag gtgtggtgac ccgctggtag
    14341 ccgtagaggt cgaaggtctc catcagctgc tgccagagca gggtccgtgt catggacgct
    14401 ggcggcagca ggtcgcgcat tccacccggg ggagtcaagg gcaggccagc attcgtgatg
    14461 ccaagccgcg ccgcactccc cggtggtcgc tttcgcgccg aagggggcat ctcagagcct
    14521 cagggtatgc acactgctga cgtagtcgat ggcgcgcagc tcgtctagag cgtctttgcc
    14581 gacgttcgaa tcgacgttcc agagcgccag agcctgtccg ctcttctcgt cgagcccgac
    14641 ctgcatccgc gacacgttga tgcctcggct gccaagcagg gttcctaccg atccgatgac
    14701 accgggccgg tcctggttgc gcatgatcag gatggagcct tccatgaccg cgtcgatttc
    14761 atagccttcc atgccgacga gccgtggctc acccttgcgg cctggcgtac ccgttgcggt
    14821 gtggatcccc ttttcgccgg tgaccgtcac acgcaccgtc gtcgaatacc ggtcgctcgg
    14881 ttccttgacc tcggcgaggc gaatccctcg ctcctgcgct tcgtaggggg cgctgatcgg
    14941 attcaccggc tcttcgagat gctgctctag gaacccggcc agggccgccc gcgcgatcgg
    15001 ggtcacgccg agctcaccag cctcaccggt gcaggtcacc ctcagctcgc gcacgtcgat
    15061 cgggtcgagc tggcctacca ggcttccaag cgccctggcc acgtcgaggt acggcttgag
    15121 gcgctctgcg gtttcctggg gaagcgctgg gacgttgacc gcgttcttga cgataccgag
    15181 ctgcaagtag gcgatcgctt gttcggcgat ttccaccgcg acgcgctcct gcgcttccgc
    15241 tgtcgacgca ccgaggtgtg gtgtgcacag gacgcggtcg agcccgagaa gcgggtgctt
    15301 cggatcgatc ggctcggtgc cgaatacgtc gagcgctgcg ccggcgatct tcccgccggt
    15361 gatcgcgcgc acgagcgcct cctcatcgac gatgccgcct cgggcggcgt tcacgagcaa
    15421 ggcgtccttc ttcatctttt cgaaagccgc atcgctgaac aagttcttgg tttccggcgt
    15481 gagcggagcg tgaatcgtta tgaagtcggc acgcgcgagg agatcgtcga gctcaaccag
    15541 ctcgatgccg agctcggcag cgcgatcgct gctcatcacc gggtccactc cgacgacctt
    15601 catcttcaaa ccttgcgcgc ggtccgccac gatccggccg atgttgccga ggccgatgat
    15661 gccgagcgtc ttgtaggcga tctcgcggcc ctggaacttg gtcttctccc agttgccgtc
    15721 gcgcatcgac gccaccgcct gcgggatgtt cctcgccaac gaagcgagga gcgccaaggc
    15781 gtgctcggcg gtcgttacgg agttccctgt cggggtgttc atcaccacga tgccgcgccg
    15841 actcgcagcc ggaatgtcca cgttgtcgac gccgatgccc gcgcgcccga cgaccctgag
    15901 tcggtgcgct tcctcgatga cccttggggt gacccgtgtc gcagatcgaa tgatgaggcc
    15961 gtcgtattcg ccgatgacgc tgcagagctc gtcttcactc atccctggcc ggaactcgag
    16021 ctcgacgccg ggcgcctcac gcaggactct gaggcccgcc tcggcgagct tgtctgacac
    16081 gaggacacgt acgttggaca tggatactcc tatggaatcg gccccaaaaa gccgggcgga
    16141 gagctagcag ccgcctaccg aggggtcaag gagcgacgac ccttggattt acatccagtg
    16201 gatggcgccg aggctcagca gcagcgaggc aacgcggtat ccgcgttgtg ggtcggtatg
    16261 gccggatcgg gcgtccacga attgcgccaa ctgctcgacc gtctgaggtg tcgtgcagcg
    16321 cttcagcgca gcttggaggt cgtccggaag tgcagcggtg gcaacggcgg tgtcgatccg
    16381 atcggcttga acgagcaagt cttgtcctct ctcgagaatg agcgcgctct cgaaaccggc
    16441 agcgattcga cgttgaagtc cgcggtcgag gatctcccaa gcgtcgagcc ggagcggaaa
    16501 gctgctgctg ggcgggtcca cgccctcgta gaacgtcgcc gtgcccgagg cccaagtgaa
    16561 caggtccaga agcttctcgc ggacctgctc cccgatatgc tgaaagagct ccaagggctc
    16621 gacgagaccg agcgccacca gggtgtcgcc aagcttgccc tcgaaccgcg gcatcaccgc
    16681 caaggccatg tcgagctcac tgcgattgat cacgcgcttc ttcacgaggt attcgccaag
    16741 gagctcgcct gcgaggttgc tcgtcacgaa cgcgggcacg ccgtgcttca catagacttc
    16801 ctttcggacg ccaccttgct cgcatagcca gaggcccgtt cgtcgcgtga ccagggtgcg
    16861 aaccagcgca gacacgaaac ctccctcttc gaagtccatg ctgcggtccg cagacgccgt
    16921 ggactcgacc cgagtggtcg tcggcgtcat gcgcgatgca ggaacgtgac gatagagatc
    16981 cttcaccgat gacaagggct gaaagtcgcc gccggcgatg ctgatgtcgt cgctgccgtc
    17041 cacttgtccc gtcgccaccg cttcgacgac cttcgcgtat gcgagaggcc cgaccgttgc
    17101 gccgttgggt tttcggatct tgtattcgag tggcgtgacg ttcgtcgtat ttgcgcggtc
    17161 gcccgccagt acgagcggag tcggcggcat cgtcgccttg ggcagcgtgt cggcagaggt
    17221 ccgccgcatg cgcgtttcgg cggcaatcga tccaacgatc gcggcgagct cggcgcgcag
    17281 gacttcttcg gggcggcttt gccatggctc gagcgcagcc cagaatgcgc gcgcggtcgg
    17341 agggcgttcc tcggctctgg ccgcaagcgc gctgagaatc acctcgccga gtccaacggg
    17401 gagctgcgga atgaattcga tgaagggatg cacttcggcg tcgcggatcg cgaggagcac
    17461 ggccagctcg ctgccgcctg cgaacaaagg ccgcctcatg aagagctctg ccgcgatcac
    17521 gccggtggag aacaggtccg cccgccgatc caccgctcca cccgagacct gctcgggagc
    17581 caaatagccg agcttgccat tcgcgcgtgc cgccgtgtcc gcctcgagcg cgttgacgtt
    17641 cgcgcttgcg attccgaagt cggcaatctt cacgtctccg tgaatcgaga tgagcacgtt
    17701 ggaaggactg atgtcgcggt gcacgattcc caacgtgctg ccgctgcggt ccttcgcatc
    17761 gtgcacggcc tcgagcccgc tgaggatctg acgaatgaga tagacgatca gatcgtgctc
    17821 gagcttcttg ctctcgagcg tgagccgccg gttgagacgc cagaggtcgc aaccgggcac
    17881 gtactcgagc gtgagatagg gtttgccatc gatctcaccg tgaccgagca cgtgaaccac
    17941 gttcgggtgt gtaacctggc tcgcgagtcg cgcctcctcg gcgaacatct gttgcgcatt
    18001 cgactcgttt gcgatgtgcg ggagcatccg cttcagcacg accacccgcg gctcgccagc
    18061 gccgcgcggc tccaatgcgc gaaacacttc cgccatgcct ccgactgcaa tacgatcgac
    18121 cagcgtgtag gcgcccagca cagtattcgg tggtggcgcc ggtggcggtg acgtaggtct
    18181 tggcacatct ggattgtaac ggtcacaacg gaaaatatcg atattaggaa aataaaacac
    18241 ggggcgatgg ggagaatttc atggcggatc caagttccag ggcaggcgag cggtatgcca
    18301 gcgcacaaat tctcgaatat gccgctcgaa cgcaccatcc tgcggacccg gcgctcctcc
    18361 gagcgttcga ggcgccagca cgccacggca tgcccgagat ccaactgggt cccgccgagg
    18421 ggaggctact cgagctcttg cttcggctcg cgggcgccaa gagggtcgtc gagatcggca
    18481 ccttggctgg ctattcggcg ctctggatcg cccgggccct gccgccggga ggacatctct
    18541 ggacgatcga agccgatcgc aagcacgcgg gcgtcgctgc ggaggtcatc ggagaggctg
    18601 gtctcgggga tcgggtgacc gtgatagccg acgaggccct ggcagttctt ccaaagctga
    18661 gtgattccgg gccattttgt gcggtgttcc tcgatgcaga caagggccga tacgacatct
    18721 atggtcgttg ggcgacggag aaccttaggc ctggagggct actcatagga gacaacgcgt
    18781 acctcttcgg gagcttgctc gagcagagcg aggatgccca agcgatgcgc cgctttcatg
    18841 aggagatggc ctcgaactac cgatcggtct gtgtgccgac cccggacggg ctcgcggtgg
    18901 ggattcgact cgattcaaag gcttgaccaa atcgagccgt acggcgatat aggcttccgc
    18961 gttcgggacc gctccaggct tgaccggttc tgggagcggc cctagcgggc cccgcaggga
    19021 gaggaaaagt cgagtgcagc ctggctgcgc aaccgccgcc tgggagttgt tcgtcaaaaa
    19081 gtgacgaacc ggcttgtccg aaccgcgagc ttcgcctaca tggcgcccgg tcgtttcgag
    19141 gactcaagag aaaacgcagc atgcagattt tcccccgcga gttgaactac ttgcccctcg
    19201 cgttggccgt cgtggccggc ggtctcgggg caggcgtgtt catattgttc tgggtttact
    19261 tcaccccacc caaccttcag gttgggtaca cgccgaagca acccgttgcg tacagccacc
    19321 gattgcacgc cggcgagctc ggcatggatt gtcgctattg ccactctcag gtggagcgct
    19381 cagccaaggc gatggtgccg ccgacgcaga cctgcatggg ctgccatgct gtggtgcgca
    19441 ccgagtcgga gaagctagcg ctggtgcgtg acagctgggc gaccgacgag tcgatcccct
    19501 gggttcgcgt ccaccagctt ccagatcacg ctttcttcaa ccacagcgcc catctcaacg
    19561 gcggcatcgg ttgtcagtcc tgccacggcc gaatcgatca gatggaagtg gtcggcgtcg
    19621 tgtcgcccat tgccatgcag tggtgcctgg agtgccaccg aaatccggag ccgcacgttc
    19681 ggccgaagac cgaggtcacc aatatgaagt gggaagccga cgaggcttgg ctcgcgcaac
    19741 gcgcggaggt cgccgctacg ctcaatccac ccttgcattg ctcggggtgc caccgatgac
    19801 acgaagaaac tcgtatgagc tgaatccggg caaaggcccg tcgatgtggc ggacgctcga
    19861 agacaagaac accgacccgg aagagctgaa gaagctcgcc gaggaggagc aacctggcgg
    19921 gttcatcacc gatctcctca gccccaaggc gctcatctcg cgacgctcgt tcgtgcaagg
    19981 ttcctcgatc gctgcgcttg ccgcaggagt tcaaggctgt gtgcggcgac cggtgaacga
    20041 gatcctgccc tacagcaagg cgcccgagta cttggtgcct ggcatagctt cgcacttcgc
    20101 tacggtcacc gcgcgcgggc gcgactcgct tggcgtcgtg gtcaccagtc acgatggccg
    20161 ccccaccaag gtcgagggca acccggagca ttcgaggagc tttggcggca ccgacgttcg
    20221 ggcgcaggcc tacgtctggg atctctacga cccggatcgc tcccagtctc ccgcgcagcg
    20281 caaaggcaat gcgctggtca atgc
//
LOCUS       DASRZF010000191         6068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2195760, whole genome shotgun sequence.
ACCESSION   DASRZF010000191 DASRZF010000000
VERSION     DASRZF010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 6068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6068
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2195760"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(225..1229)
                     /locus_tag="VFG22_08430"
     CDS             complement(225..1229)
                     /locus_tag="VFG22_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416310.1"
                     /translation="MRWRSGMHAVVAAGLWLVASSAGADDTHYRSVPIGAHAIGLGGA
                     FTGVVDDASAAFYNPGGLMLDATTGIAGGLTINAWDKVEIDNALENPDGVAKSNATGA
                     RTIPLFIGALVKFGPENVFKQKKFALALSVVEPNFSREGDFLELPSDPVEFTTTYTIA
                     VNDRATWYGLSFASRINLKHMIGTSLYLSVRRLNQNELGLALAGGTQIATDPAQFVGT
                     TSAATNQRLNFKAFHLALRFGWLFRFKPQLQFGVMLQPPGIPLKQRVGAVSQEFLNDN
                     QDPSSPLTTTVAYLNDDFDANLPIPLELRTGVQYWPAEKVMLALDAMFYGPVRSGQRV
                     "
     gene            1393..2538
                     /locus_tag="VFG22_08435"
     CDS             1393..2538
                     /locus_tag="VFG22_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416311.1"
                     /translation="MAGQAKTNRTPNRLVDRLVQHGEVDSFRELCTRVLTAKTRYLVL
                     DLDRTIHLGRDPGQDLGWELCAYQGYGREHFERIEHRQESGRFLLDWDHPRKTAQYLA
                     RSLKIWAYPGVYYGVWGKAAARLDWLRRRGFEHFVADPVRAAQRVPQLTLLRHLQTAA
                     EDVLRELAKQIWMRHEPDQVIDREDLDWVRSQWPEIEIVLSSASPKPTVEVAGEALGV
                     NHVHYSTLDRINSGEAKIERLREICPRVGEPDVEIVGISDTSRGEDHCWVDHFTKVVD
                     INSPTPFPAIVSSASPLLEVHSATLLSKYERQRRAAGDPSYLDPRREKLALSPSKRDL
                     RRDDLERRLGWLLKRVNALASAPGQISGDMAYRLAVLREASTTLVRA"
     gene            complement(2561..4054)
                     /locus_tag="VFG22_08440"
     CDS             complement(2561..4054)
                     /locus_tag="VFG22_08440"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HET6416312.1"
                     /translation="MDGIWIVLLTLLAILGAPLFAIFGAAAMLLFGALPDTSITGTAN
                     DVFSEKFADSPLLVTIPLFTFAGYVLAESGTPHRLVKLSRAWLGWMPGGLAMVCLVAS
                     AFFTTFTGGSGITIVAIGGLLYPALVADKYDENFSLGLVTTGGSLGLLFPPSVPMIIY
                     GVVAGIMIEKLFLAGLIPGIITVAALAIYSSITGIKHKMRRTRFDAKEAVRSLWEAKW
                     EALLPVALLGGMYSGLLRIHEAAAFTAIYTLIIEVWVYQDVSFRKDLPRIIRESMTLV
                     GAILAILATAIGFTAFLIQARVPMMILDAMEGYITSQVMFLIALNFFLIGVGMLMDIF
                     SAIVVVVPLIVPIALRFGVDPYHLGIIFLLNLEIGYMTPPVGLNLFISSFRFNKPITQ
                     LYRAVLPFIGLLGLALIIVTYVPALSTWIPNMVESNAVNLNEPGDEGGDDFGMDLDAL
                     EDDNADSLDLDSLEGDDEELDLDSLEGDDEELDLDGLEGDDESPDLE"
     gene            complement(4058..4735)
                     /locus_tag="VFG22_08445"
     CDS             complement(4058..4735)
                     /locus_tag="VFG22_08445"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HET6416313.1"
                     /translation="MNRIRRLDRGLARGEAAIAATVLLLMIGVAATQAALRNLTNLDF
                     TWANLALERMEWADSFLQKGTLWLAFFGASLSTFDEKHIAIDVLPRLSPPRIKQLLRA
                     IVCVFSAVTCFYLGRVFWLSVLNNAREIPLEYSVLGATDQMIHICAAPLDLLADAGLS
                     KPTIFCAVRSALDVFGAEMSTPDVALQLIVPAMFMFMAIRFLSRAVAASAAFITKNYP
                     EESNGEG"
     gene            complement(4772..5695)
                     /locus_tag="VFG22_08450"
     CDS             complement(4772..5695)
                     /locus_tag="VFG22_08450"
                     /inference="COORDINATES: protein motif:HMM:NF028121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HET6416314.1"
                     /translation="MSCRLRSAAGLFCVLVCVSALGACDVAKFTADSTAGLFTRAAPA
                     FESYWDYELAGEALPATVVQFEGILRVVPDNEAILAQLAQAYIAYAYGWVEADVESME
                     FDGDYEEADVQRQRARIMYLRALDLTRHWVRLHDPDVDVAVKATVAELEEWLKSAFVQ
                     KADAEMLLWHGYAWGSYINAAKDDMEAVADLEYAKAFVARSIELDPDFYNAAGYTFMG
                     VATASEMAGDMDEAKVYFEKALERTERRALQIQLNMARYYAVKEGDRELFDTLLAEVM
                     DAQDPLPEARLANRMARVRAALYIENADQLF"
     gene            5807..>6068
                     /locus_tag="VFG22_08455"
     CDS             5807..>6068
                     /locus_tag="VFG22_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416315.1"
                     /translation="MLRFRSLPVILGPVLAALLFTSPAHGSIVQGLDLEELVSNADRI
                     VLGRVVLSESFPRPNGQIATWHRIEVKRELRGHAPDETEVIVE"
BASE COUNT         1232 a         1855 c         1906 g         1075 t
ORIGIN      
        1 agtccgatcg atatggtgcc cgcgtaccga ttgatttgcg gattgtagta ccgatcggga
       61 ttatcaggga tgttgatcgc cgaggaaaag tcggtgaaga agcccgtctc gatcatgatc
      121 ttcttgctga tgaagaaatc cgcgccgacc gcgacgttcc cgatggcgcg gcgcgcggta
      181 ttgttgtcgt agaacaggcc cccgaccagt cccgacgagc cgatttacac gcgctgcccc
      241 gagcgcacgg ggccgtagaa catcgcgtcc aaggccagca tgactttctc agccggccaa
      301 tactggacac cggttcggag ctccagaggt atcggcagat tcgcgtcgaa gtcgtcgttt
      361 aggtaggcga ccgtggtcgt gagaggcgaa cttgggtctt gattgtcatt gaggaactct
      421 tgcgagaccg cgccaacgcg ttgcttgagc gggattcccg gaggctgcag catcacgccg
      481 aactggagct gcggtttgaa tcgaaagagc cagccgaagc gaagcgctaa gtggaacgcc
      541 ttgaaattca gtcgctgatt ggtggcggcg ctggtggttc cgacgaactg ggccgggtca
      601 gtcgcgatct gtgttccgcc agccagcgcc agcccgagct cgttttgatt gagcctgcgt
      661 acgctcaagt agagagatgt gccgatcatg tgcttcaagt tgattcggct ggcgaatgaa
      721 agcccatacc aagtcgcacg gtcattcacc gcgatcgtgt acgtggtcgt gaactcgacg
      781 ggatcggatg gcaactcgag gaagtcgcct tctctgctga agttcggctc gaccacgctg
      841 agagccagcg cgaacttctt ttgcttgaat acgttctcgg gtccaaactt gacgagggcg
      901 ccgatgaaga gcgggatcgt gcgagcgccc gtcgcgtttg acttggccac gccatccggg
      961 ttctcgagag cgttgtcgat ctcgaccttg tcccaggcgt tgatcgtgag accgcccgcg
     1021 atgcccgtcg tcgcatcgag catcaatccg ccagggttgt agaacgctgc ggaagcgtcg
     1081 tctacaacac ccgtaaacgc gccaccaagc ccgatggcat gcgccccgat cggcaccgaa
     1141 cgataatggg tgtcgtcggc gccggcgctg ctggcaacga gccatagacc ggcggccacg
     1201 accgcgtgca tacccgagcg ccagcgcaca gtccttcttc tagacaatga ccgccccatc
     1261 gcaagcgatt tgtaccatcg gtccgggccc ccgccctcgc gaatcgatcc attccgagct
     1321 tgtgaaccga ggacgccagc gtttgaccga acttttacaa acgacccgcc aaagtcgcct
     1381 tagattgaag ctgtggcagg gcaagccaag acgaatcgca ctccgaaccg cctggtggac
     1441 cggctggttc agcacggtga ggtcgattcc tttcgtgagc tttgcactcg ggtgctcacg
     1501 gcgaagaccc gctacttggt gctcgacctg gaccggacga ttcacctcgg ccgagatccg
     1561 ggccaggacc tcggctggga gctctgcgca taccaggggt acgggcgcga gcatttcgag
     1621 cgcatcgaac accggcagga gagcgggcgc ttcctgctcg actgggacca tcctcgaaag
     1681 acagcgcagt acctcgcgcg cagcttgaag atctgggcat acccgggcgt gtactacggt
     1741 gtctggggaa aggccgcggc acggctcgat tggcttcggc gtcgcggctt cgagcacttt
     1801 gtcgccgatc cggtacgggc tgcccagcgc gtcccccagc tcaccttgct ccggcacctg
     1861 cagaccgcgg ccgaggacgt gttgcgcgag ctcgccaagc agatctggat gcggcacgag
     1921 cccgaccagg tgatcgatcg ggaggatctc gactgggtcc ggtcgcagtg gcccgagatc
     1981 gaaatcgtcc tcagcagcgc ttcccccaag ccaaccgtcg aggtcgccgg cgaagcgctc
     2041 ggcgtgaacc acgttcacta ttcgacgctc gaccggatca acagcggtga ggcgaagatc
     2101 gagcgattgc gcgagatctg cccacgcgtg ggcgagccgg acgtcgagat cgtcggcatc
     2161 agcgacacca gtcgcggcga ggatcactgc tgggtagacc acttcacgaa ggtggtcgac
     2221 atcaacagtc cgaccccctt cccggcgatc gtgtcgagcg cttccccgct gctcgaggtt
     2281 cactccgcca cgctcctgag caagtacgag cgtcagcggc gcgccgccgg agacccgagt
     2341 tatctggacc cgcggcgcga gaagctggcg ctcagcccgt cgaagcgaga tctccgacgc
     2401 gacgacttgg agcgtcgact gggttggctg ctcaaacgag tgaacgcgct cgcgagcgcc
     2461 cccggacaga tcagcggcga catggcctat cggcttgccg tgctacgcga agcatcgacg
     2521 accctagtgc gcgcctgagc gcggcacgaa gagcctccca ctactcgagg tcgggcgact
     2581 cgtcgtcccc ttcgagaccg tcgaggtcga gctcctcgtc gtcgccttcc aggctgtcga
     2641 ggtcgagctc ctcgtcatca ccttccaggc tgtcgaggtc gaggctgtcc gcgttgtcgt
     2701 cttcgagggc gtccaggtcc atcccgaagt cgtcgccccc ttcatcccca ggctcgttga
     2761 ggttcacggc gttgctctcc accatgttcg ggatccaggt cgagagcgca ggcacgtaag
     2821 tcacgatgat cagcgccaga ccgagcaggc cgatgaatgg caggacggca cgatagagtt
     2881 gtgtgatcgg cttgttgaat cggaagctcg aaatgaagag gttcagaccg accggcggcg
     2941 tcatgtagcc gatctcgaga ttgagcagga agatgatgcc gagatggtac ggatcgactc
     3001 caaaccgaag agcgatcggc acgatcagcg gcacgaccac gacgatcgcc gagaagatgt
     3061 ccatcagcat gcccacgccg atgaggaaga agttcaaggc gatcaggaac atcacttgtg
     3121 aggtgatgta gccctccatc gcatcgagga tcatcatcgg cacgcgggcc tgaatcagga
     3181 atgccgtgaa cccgatcgca gtcgctaaaa tcgccaggat ggcgccgacg agcgtcatcg
     3241 actcgcggat gatgcgcggc aggtccttgc ggaagctcac gtcctggtac acccagacct
     3301 cgatgatcag cgtgtagatc gccgtgaacg ccgcggcctc gtggatgcgc agcaggccgc
     3361 tgtacatacc gccgagcagg gccacgggaa gcagcgcctc ccacttggcc tcccaaagcg
     3421 accggaccgc ttccttggca tcgaaccgag tccggcgcat cttgtgcttg atcccggtga
     3481 tcgagctgta gatcgcgagc gcggcgacgg tgatgattcc cggaatgaga ccagcgagga
     3541 agagcttctc gatcatgatc ccggcgacga cgccgtagat gatcatcggt acgctcggcg
     3601 ggaacagaag gccgagggac ccgccggtcg tgacgagacc gagcgagaag ttctcgtcgt
     3661 acttgtctgc cactagggcc ggatagagga gcccaccgat ggcgacgatg gtgatgccgc
     3721 taccacccgt gaacgtcgtg aaaaaggcag atgccacgag gcagaccatg gccaggccgc
     3781 ccggcatcca gccgagccag gccctcgaga gcttcaccaa gcgatgaggc gttccgctct
     3841 ccgcgagcac gtagccagca aaggtaaaca agggaatcgt gacgagcagc ggagagtcag
     3901 cgaatttttc gctgaagacg tcgttggctg taccggtgat gctggtgtcg gggagcgctc
     3961 cgaagagaag catcgccgcc gcaccgaaaa tcgcaaagag aggcgcgccc aaaatggcga
     4021 gcagggtgag gagcacgatc caaatgccgt ccatcgctca gccctccccg tttgattctt
     4081 cggggtagtt cttggtgatg aacgctgcgc tggctgcgac ggcgcgactc agaaagcgaa
     4141 tcgccatgaa catgaacatc gcagggacga tcagctgaag ggcgacatcc ggagtggaca
     4201 tctcagcacc aaacacatcg agggcgctgc gaaccgcaca gaaaatggtg ggtttcgaaa
     4261 gcccggcgtc ggctagcagg tcgagagggg ccgcgcagat gtgaatcatt tggtcggtag
     4321 cgccgagtac cgagtactcg agtggaatct cgcgtgcgtt gttgagcacc gagagccaga
     4381 aaacacggcc gaggtagaag caggtcaccg cagagaagac acaaacgatc gcgcgaagta
     4441 gctgcttgat gcgaggcgga gagagccgtg gaaggacatc gatggcgatg tgcttctcgt
     4501 cgaaagtcga aagcgatgcc ccgaagaacg caagccagag agtgcccttc tgcagaaacg
     4561 agtccgccca ctccatccgc tccagggcga ggttggccca agtgaaatcg agattcgtga
     4621 ggttgcgaag cgcggcctgc gtcgcagcca ccccgatcat gagcaggagg acagtggcag
     4681 cgatggctgc ctcgccgcgg gcgagccctc tgtctaggcg ccggattcta ttcacgcttg
     4741 cttctccgat ggggccgtcg cgccgatatc actaaaagag ttggtccgcg ttctcgatgt
     4801 agagcgccgc tcgaacgcgc gccatgcgat tggcgagccg ggcctccgga agcgggtcct
     4861 gggcgtccat cacctcggcg aggagagtgt cgaagagctc ccggtcgcct tctttcacgg
     4921 cgtagtagcg cgccatgttg agctggatct gcagggctcg ccgctcggtt cgctccaatg
     4981 ccttctcgaa atagaccttc gcctcatcca tgtcgcccgc catctccgac gccgtggcca
     5041 cgcccatgaa cgtgtaccca gccgcgttgt agaaatccgg atcgagctcg atggaacgcg
     5101 cgacgaacgc ctttgcgtac tcgagatcgg cgacagcttc catgtcgtcc ttagccgcat
     5161 tgatgtagga gccccaggcg tagccgtgcc aaagcagcat ctccgcgtcg gccttctgca
     5221 cgaaggcgga cttcagccat tcttcgagct cggcgacggt cgccttgacc gcaacgtcga
     5281 catccgggtc atggagcctc acccagtgcc gcgtgaggtc gagcgctcgc agatacatga
     5341 tgcgcgcgcg ctggcgttgc acgtcggctt cctcgtagtc gccgtcgaac tccatggact
     5401 ccacgtcggc ttccacccaa ccgtaggcat aggcgatgta ggcttgagcg agctgcgcca
     5461 aaatggcctc gttgtcgggg acgacgcgta gaatgccttc gaactgcacg accgtcgcgg
     5521 gaagcgcctc gcccgcgagc tcgtaatccc agtacgattc aaacgccggt gccgcgcgag
     5581 tgaacagccc cgccgtgctg tcggcggtaa acttcgcgac gtcgcaagcg ccgagcgccg
     5641 agacacaaac aagaacgcaa aacaggccag ccgccgatcg cagacggcag gacatcagat
     5701 cctggtacat agagcgaggt gtacgaaatt tagggggaaa ttgtcaatcg agatcgtgtg
     5761 gggtcgatcg cttgacggcg ccaagcgcgg cccgcagcat taggaaatgc ttagattcag
     5821 gtcgctgccc gtgatcctcg ggccagttct tgccgcgcta ctgttcacga gcccagccca
     5881 tgggtccatc gtccaaggcc tggacctcga ggaattagtc tccaacgccg accgcatcgt
     5941 gctcggccgt gtggttcttt ccgagtcgtt cccgcgcccc aatggccaaa tcgccacctg
     6001 gcaccggatc gaggtcaagc gcgagctccg cggtcacgcc cccgacgaaa ccgaggtgat
     6061 cgtcgaaa
//
LOCUS       DASRZF010000192        33595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2196100, whole genome shotgun sequence.
ACCESSION   DASRZF010000192 DASRZF010000000
VERSION     DASRZF010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 33595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33595
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2196100"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VFG22_08460"
     CDS             complement(<1..463)
                     /locus_tag="VFG22_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HET6416316.1"
                     /translation="MRYLPHTDQEIRAMLTRVGVEHIDDLFAAIPASHRLDRELDLEP
                     ALDEPRLMQHLAALAQASTGANMLSFLGGGMYRHHVPPAVDQLLQRSEFYTAYTPYQA
                     ELSQGTLQAIFEFQTVVCELFGTDVANASMYDGASGAAEAVLMARRIKRRTH"
     gene            complement(479..871)
                     /gene="gcvH"
                     /locus_tag="VFG22_08465"
     CDS             complement(479..871)
                     /gene="gcvH"
                     /locus_tag="VFG22_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HET6416317.1"
                     /translation="MATYPRDLQYTREHEWARIEGDLIRVGITSYAVEQLGDVTLVDM
                     PAPGEDVQAHERFGDIESVKTVSELFAPVSGEVVEVNGALESSPELVNEGPYEGGWLI
                     MIRPSDQGELQGLMDAAGYEEYLGTLDS"
     gene            complement(874..1968)
                     /gene="gcvT"
                     /locus_tag="VFG22_08470"
     CDS             complement(874..1968)
                     /gene="gcvT"
                     /locus_tag="VFG22_08470"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HET6416318.1"
                     /translation="MTESLRETSLTDEHRALGARLVPFAGWLMPVQYAGIKKEHDAVR
                     SRAGLFDVSHMGEILVEGPRALSEVDRLVTNDVSKLAVGSALYTACCNEAGFILDDLI
                     VYRLEDEKVLVVCNASNRGKIVTHFSKNLSLQTPWNDVSDAWALLALQGPNAVSVMAS
                     LGASSELTGLPYFRVARATLAGVDLWVARTGYTGEDGFELFCASDDAVKLWRALLEAG
                     QPHGLEPVGLGARDTLRLEAKLMLYGNDISEETNPFEAGLGWVVKLDAGDFIGRDALR
                     GIKAAGLQRRLVGFEMTGRGIARHGYPIVADGERIGEVTSGSPGPTVGRNIGLGYVPL
                     ALAKVGTNLGIEIRGKVIDAVVVTTPFYKR"
     gene            2036..3580
                     /locus_tag="VFG22_08475"
     CDS             2036..3580
                     /locus_tag="VFG22_08475"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET6416319.1"
                     /translation="MVQDDILWLCRGGRRIRSFRVGAGAIEVGSHPGCDIVVHAAAVP
                     ARALLFRAHGGTVQVYDLAKHPGLGSPALMPIGGQLSVGAGYSVLRVKGEKVEPRSVS
                     TEILGRHQSESRRLSLLSGQGAGARAYHLGDEPLSIGCAPDNAISLPDRAVSRHHCRL
                     EPSTGGVSVRDLGSTNGTWVDGLRIGHQALRPGALLRIGRTELRVVGRSGGRDAFGDL
                     VVESNLMLSVMAQVERLAALPWPVLVCGETGVGKEHVARALHERGPRRKGPFVPLNGG
                     GLPRELIESELFGHEKGAFTGAVRARRGAFEEAHGGTLFLDEVAELPADLQTRLLRVL
                     ETWHVRRVGGESARRVDVRLVCATHRDLRAMVHEGRFRPDLYYRIHRLVVEVPPLRAR
                     AGDIAPLARHFIRRMESELGERQLDPHALERLRRYPWPGNVRELRNVLELAAVHCPSV
                     VLDLEAIDRALQRCAEPSVLRPSGDSLREALEHYGGNMSAAARALGIPRSTLRDRLRS
                     PAESEG"
     gene            complement(3577..4290)
                     /locus_tag="VFG22_08480"
     CDS             complement(3577..4290)
                     /locus_tag="VFG22_08480"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET6416320.1"
                     /translation="MNGLDLSVVIFAFNEEANIAPVLLELREWLGANETSAEIVFVDD
                     GSSDGTSAEAARALSGAPHQLLRHETNRGIGAALKTGVRAAKGAWVTFLPADGQIAPE
                     AIGTLRAAAARDQSEVVFSVYDHRNDGVHRKILSAGVRGLIFLVHGVRMQSDGPYLFR
                     RPLFDADQLVPDTFFLNFEFPIRMLGAAVPNSVVTIQCRPRRAGHSKSTGLKRIAGVA
                     QDLFELRIRRAKESFARNA"
     gene            complement(4287..4805)
                     /locus_tag="VFG22_08485"
     CDS             complement(4287..4805)
                     /locus_tag="VFG22_08485"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET6416321.1"
                     /translation="MRHRSHGDGSFRADQFARCGADCVFEAGVLVFHPERIELGTNVY
                     VGHHAILKGYHKNTMRIGDETWIGQQAFLHSAGGIDIGARVGIGPGVRIITSFHGEAG
                     RDVPILSSPIELAGVVIEDDCDLGVGATVLPGVRIGRGAQIGAGAVVTEDVPPYAVAV
                     GVPARVTRERPE"
     gene            complement(4809..6779)
                     /locus_tag="VFG22_08490"
     CDS             complement(4809..6779)
                     /locus_tag="VFG22_08490"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HET6416322.1"
                     /translation="MTDRILGLPVQSRRATAAATAALFAYYVATMCRSFSMYDSPELA
                     LVAEQLGLGHPFGQPLHTLLGALAVRLPGLDPLIALNGLSALAGALTVIPATSLAQTL
                     VRPDPRCPPGDLRFISPAVALAGVHTALWEPSTRIEVYSLAVFFVLWAAARCSNALLD
                     QDQRSRLYFSVGLAFGLAASANVVCAFGGAIAITPRLLIAVFRKEIPRRALALLIGGG
                     LLGLATYAYVFLVAGREDVVVWGAPTDAAAIKHYFTAADFSAKGVTSWAEWWDHMKQL
                     FFWSLQNGLFALLLTGFAGYAIYARRRGLGRFFFNATLLFFAAFIARNGVFATDVLDY
                     NGYLAIPAWIAASGVGLLVAYLGARKPQFAVASLAAVLLLVLTAPPGPAHRTRHRDKF
                     TADIAREALAAAPRDAIVIVRDDHWIGPMWYLQEQTGLRPDITLLAYGLSASGWYWDH
                     LYRRHPDLALITLRGPGGRDARVLRFLRANPGHPVQIESVALADQLGLPTCPSDWMLD
                     VRTGCAPDSHERSLGGYARAALAELGLGSPGTDGLIAMITLDRGHDLYSQGFPRAAIA
                     TLLSGVPRTDGIEEPNLDSVPERIPRLIRPVPSYDPPVALGHPAQNLHYASIIAHETG
                     ATGLGIYFAGLSAAMGPVQPKFASRPAAPANL"
     gene            complement(6772..7746)
                     /locus_tag="VFG22_08495"
     CDS             complement(6772..7746)
                     /locus_tag="VFG22_08495"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HET6416323.1"
                     /translation="MHDNGERMNSSGERRRRYLRWGRIAVTIAAVAYLASRVHPRDVL
                     KAFERLSPGTALTAVSVVFLGLFFGMIRWKLLLRAYGAIDIPSWKRIAHLYFVGHFYN
                     TYAPGGMGGDILRGVAGRKAFGDIDWASATSGVAVVFIERVIGVSALLLLAAGAYLLH
                     PSAGIENVGLWAGLGLAGAAFAMLGIAVAPRLAPHLPEKLGKPLRALPRLYSFWPFLG
                     AIVLSLLIHFVNVVAGHAIMHSLEPSVQFAESAVVMPLISASAFFPFTVGGAGVREAA
                     FAALYGTVGVPEASAYAASLGFWATQLFSAGVGGVINLFVPLPGRDGD"
     gene            complement(7748..8374)
                     /locus_tag="VFG22_08500"
     CDS             complement(7748..8374)
                     /locus_tag="VFG22_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416324.1"
                     /translation="MNFFGHAVVAGWADKRPGHLLGSMLPDFEGMLGIRLVEVRDAQI
                     RCGIELHHQTDEAFHRTPSFLAFCSYALDELTLRGVRRGTARAVGHIGSELFLDGWLT
                     RDRTHVDDYLAALAVEADGALRWEDEGQAFSALHRRLAIWGAPRDYAEPDFVLARLSD
                     ALRRRPALELLEEHSSSVAAFLPSLRERVECGASELLVELRNALGFEH"
     gene            complement(8371..9303)
                     /gene="miaA"
                     /locus_tag="VFG22_08505"
     CDS             complement(8371..9303)
                     /gene="miaA"
                     /locus_tag="VFG22_08505"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HET6416325.1"
                     /translation="MTEPLWPILVVAGATGTGKTSVAIELARHFGGELVGADSVQVYR
                     GFDIGAAKPTASELGDVRHHLIDVFDPDQDVDAMTFAKLADTAIGEIRGRGHLPIIVG
                     GTGLWIRALVRGLVAVPPVDPALRSRLETAARNEGAAALHAKLTEVDPLAAELIHPND
                     ALRIVRALEVYEQTGTALGALRAAHALGAPRYQAMFVVLEMDRSLHEALIERRAEQML
                     GAGWVDEVRALRERWGDQVRPLGSVGYRDVLAHLRDGISLDETLRRVRKSTRVYARRQ
                     RTWFKGEPGVSWRCESDELRSPEGLERIAKELKR"
     gene            complement(9296..10960)
                     /gene="mutL"
                     /locus_tag="VFG22_08510"
     CDS             complement(9296..10960)
                     /gene="mutL"
                     /locus_tag="VFG22_08510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HET6416326.1"
                     /translation="MSPTVRLLGPTLIDQIAAGEVIERPANLVKELLENAIDARAGAI
                     TVSVEDGGRGQVRVTDDGIGMSPEDAELCIQRHATSKIASFEDLVQVRSLGFRGEALP
                     SIAAVSKMTITTRPKEDLAGTRVLVEGGDLREISPSGCPPGTTVEVQDLFYNVPARKK
                     FLRARQTETSRIFEVCQRVALGRPELRLQVTSDGRTARQYLPARSLAERAYQVFGEVA
                     LQEIHAERDGVLLDALLAPVEYARAGARHLYLFVNGRPINDRGLARAVAFAFGDRLPP
                     GQYPRGLVSLRLAPEDVDVNAHPQKTEVRFRNTAQLVDRVTRMIAARLPAHRPGDAYW
                     EARIGAHGPIEGRATEAPGSAPPSTLAEPIEVFRPRYSNGLRFVAQVRDRILICESKE
                     ELVVLDRARAETEVRYDALRQASEAGTLPRSNLLFPDRLDLNGSEQSSLERHEAILRS
                     LGFDWSALGERSYVLRAIPALLAEARPPALFKGAIASLGSGAEPVFGTTLLALAKTAA
                     TPNGESLDERGVQAIVANVRLSDDRHRACILARVTLPSPAAGIADD"
     gene            11085..12671
                     /locus_tag="VFG22_08515"
     CDS             11085..12671
                     /locus_tag="VFG22_08515"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6416327.1"
                     /translation="MKVCPACRVDYGGGEVFCPVDATRLVTTSQMTVNPLNPDDPLIG
                     RVLAGKYVVKRRIGEGGMGLVYEGIHRDIDKQVAIKVLRDDLSRRPEVVARFRREARS
                     ASRIGHEHIVDISDFGETTRGASYFIMEFLDGEDLGNVLGREGTVDAERACKIVLQCC
                     RALSATHDKGIVHRDIKPENIFLTTRDGRNDFVKIVDFGIAKMSDVETDGAPGRKLTK
                     TGMIFGTPEYMSPEQAAGKELDHRVDVYALGIILYECLAGRVPFEGDTFMGVLTQHLF
                     AEVPPIAELNSNAKPSRELELVIRKSLAKDPDDRYQDTNELAEAIECALDGRLSRATV
                     RTPPSAFGPPSLAYLDGVPRRKASRWVWALSILGALGAIGWVALGPASGPASEAGASD
                     VPAQAMRAPIERVPDLEESPSADVESAETLDEPVLVRVHVTTDPTGAAVLLEDGSKAC
                     SPTPCTVEAAPGASLVLRAKLGRRRGQTAITPTDEETVLIQLEVPKRAKPSPRSAAAP
                     RRERERPVRSSDLKVPEWAQ"
     gene            12659..15046
                     /locus_tag="VFG22_08520"
     CDS             12659..15046
                     /locus_tag="VFG22_08520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HET6416328.1"
                     /translation="MGTVSEVERKTLADLEWEQVEWAVAERCRGPLANGLKLPLAESF
                     AETERALAEAKEAFSLLTRNEPLPLEGICEIGVSLQHLEREGVLDGSALKDIRTTLRA
                     AAELRRFLGQRKESVPYLHRACAIDPTLESLEEELGMSIGDDGSILDSASAELARLRE
                     EAGNLRARIIRKLEDLIQKRSAILQDSFYTIREGRYVLPVRADAHERFHGIVHGSSAS
                     GATVFVEPRELVERGNRLKMVQAELEREERRILAALADLVRQYVPQMRAAAEALDHAD
                     LRGASARLAVDLGAEFIELSEQPRISLRGARHPLLVLRGIDVVANDLDLSGGEALVIS
                     GPNAGGKTVALKMLGLAALMIRAGLPVPAEEGSVCGFFSPVLAAVGDEQSLERSLSTF
                     SAHLVTLRGVLELAGERVLVLLDELAGATDPEAGASLACAIIEALVERGTAVAVTTHY
                     EALKALAAEREDMRNAAVGFDFDNMAPTFRLAMGVPGGSNALEIARRYGIPGEIIEAA
                     RSALPEHSRSFETLVAKLEAATKAVLEERTSLQEMRTRLSEESSKLDARQAALKAREA
                     KGLGKEAQELVDQVRETQRRLERAKKDLRERAEAESIALAGATLRDAGAVLGDLDAHR
                     LSLEPEPVETSALDVEAVRVGDRVWVDRLRSAADVIEGPARGKVRVSAGMMKLWVDLS
                     DLRALKQSGLAPTKDRTAQSGAHPKPQKSIRTSDNTLDVRGLRADDAVALAEAFLDQM
                     YGAAEPNVFIVHGVGSGALRHAIHEHLARDASYVAGFREATQDEGGPQVTVVSLK"
     gene            complement(15050..15910)
                     /locus_tag="VFG22_08525"
     CDS             complement(15050..15910)
                     /locus_tag="VFG22_08525"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HET6416329.1"
                     /translation="MHAFLFIPWFRLEAWDIPLPFSLPVLGDTLSIQPFGVLVATGVL
                     LGAWVAGRFAQRNGLDTVATGDLVTYAVVTGFILGYFLNGLFYERETLVEVLRNPSMF
                     FTTWLGLSSYGGFFGGILGCFIWKYRKKTPLLPYANAVCFGLPFGWLFGRLGCFVVHD
                     HPGKVTDFALAVADYRFGNPPFEARHDLGLYEVLFSISIIALFLWLEHRKRRPVGFYC
                     VLLPLLYAPVRFFLDFLRATPLEGGDVRYGGLTPAQWSSILMVCLGLAVWQLGVKPRM
                     AEGAPASSEI"
     gene            15985..17262
                     /gene="eno"
                     /locus_tag="VFG22_08530"
     CDS             15985..17262
                     /gene="eno"
                     /locus_tag="VFG22_08530"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016387099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HET6416330.1"
                     /translation="MTEIIAIGAREILDSRGNPTVEVDVELMGGASGRAAVPSGASTG
                     EHEALELRDGDPSRYLGKGVLKAVDNVLTEIAPQVIGHDALDQAAVDQVMLDYDGTPS
                     KAKLGANAILGVSMAVSRAAADAVGLPLWRYLGGVQAQVLPTPLMNIINGGAHADSGL
                     EIQEFMVVPHGFDTFSDALRAGAETFHNLKKLLKEAGHSTGVGDEGGFAPHLSTNEEA
                     LAFVARAIEAAGYRPGEQISIALDCAASEFYDKKTGVYTFDKKPVDAAGLVETYSDYC
                     SKYPIVSIEDGMDENDWDGWKVQTDVLGARVQLVGDDLFVTNTERVQIGIDRGIANAI
                     LIKVNQIGTVTETLHTIRLGALHGYASIISHRSGETSDTFIADLAVATGAGQIKTGSA
                     SRSERIAKYNQLLRIEEQLGEAAVYARKSTFAA"
     gene            complement(17280..18422)
                     /locus_tag="VFG22_08535"
     CDS             complement(17280..18422)
                     /locus_tag="VFG22_08535"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HET6416331.1"
                     /translation="MFEKVLILRRGEAATRVARTCRRLGVESIVVAPKNETASRHIEA
                     ADRIIEVDFEEARGIPTDQLPAILEQAGADAAHLGYQGQPEMFELAAAAEKADVAVIG
                     TDLDVLGALTDQASVKAAADRAEVRTVHDRPDPSRPRAIGVIVIADSHGNTTAIAECD
                     RSLSLEGHTLIHETPSPELLFRSDGEAFRMALFESARRLAAELRYAGLLEIQFQIDPS
                     GLFWVSDVRIGLPRHHTLIEMVMGVDLVALQLRIASGEAIPEEIETIEPRGHALDASV
                     LALDQQEAVVSSYSVSPAPQGSVRATSSATVGLPLPADDRPLIAKVTTFEPIRHRALL
                     SMDRMLAEMRVQPFATNVHILRKILSDYAFRAGQYDSESSKRFAVG"
     gene            18506..18949
                     /gene="ybeY"
                     /locus_tag="VFG22_08540"
     CDS             18506..18949
                     /gene="ybeY"
                     /locus_tag="VFG22_08540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HET6416332.1"
                     /translation="MPVWLRSRGLERRSVRHSELRWRAEAMLESLGLGQAELSILLCD
                     DESIRRLNRRYRKKNRATDVLAFPMQEGEGPIVHPGLLGDVVISLPTAIVQAAAHDRP
                     IVDEVTFLLAHGLLHLLGYDHATKREELEMSARTRDLLAAVEAAR"
     gene            19337..19651
                     /locus_tag="VFG22_08545"
     CDS             19337..19651
                     /locus_tag="VFG22_08545"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET6416333.1"
                     /translation="MTAGKRMTKAQIMSELADKSGLTKKEIGAVFDALRDLVKKELGR
                     RGTGEFVIPDMIKLKVRKIPAKPARMGRNPATGEEMMLPPKAASKKIRATPLKKLKDL
                     VL"
     gene            complement(19686..20993)
                     /locus_tag="VFG22_08550"
     CDS             complement(19686..20993)
                     /locus_tag="VFG22_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416334.1"
                     /translation="MRTGQYLDPFSTAFGIVASTLLHIAALAYISSATAHYDFDFDLT
                     LPAEVEFGLTGEMQMAAASEPPRATGVPDPRWAREAVEGGEDHPPDAGVLPDTDAEPD
                     TDTDTDTDTGADTDADTGADTDADTDADTDTVVAPPTLSGPSRIPPGAQLALRVDLKR
                     VRQSTLAPDVTRFLRGVPDWQLLLDGSGIDPVADLDRLLVATPNLERSRLVLAGRHGR
                     EDGFARQSVNRLARSRGKSVRWQRRYGVATAPWHNRDKTLRTIAVLGPKHFSITRKQD
                     LERVLAMAQARELRDAKKEGLVAARGPEALLSMGPEEVISLEIEGVHQFILGNIKHVP
                     IRLRLAVRESAPNVVTVSVLATYPSNRLAQEASVYWRKVAMFYSQQLIMSLAGFGKTL
                     RRMDFKPVDERIEVSFELNADQIRFILSYVEGRLRGSQTKKGP"
     gene            21048..22448
                     /locus_tag="VFG22_08555"
     CDS             21048..22448
                     /locus_tag="VFG22_08555"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET6416335.1"
                     /translation="MSIRSLVFVMVAASSLPLASGGCDLDEPFELIVNKRPPEPPDLG
                     PPQRVFAKRFVSPIRSAPSPEAERVGYLRAGALLHSKTSKPLGYEGCEGGWYELDTGG
                     FACNARDVIVFSGERLPELRARQADRDAVMPYEYVTVRRNTPVYKRIPRAEEIYEIPV
                     DAPDAGADAAEREASEARGPLDNPLVVRVLQPGFYVSLDRSFERDGVTYWRTQQNGFL
                     PGDHLRHKKWSEFKGQELSVGDWGLPVAVTRSDETTVYRMNERGKLRATRGRLDKRAW
                     LQVRSRQRIGDAAFLMLQDGTLVRETDVLLIRPSAPPEGVPEGERWIDVDLEHQVLVA
                     YDGMRPRYVTLVSTGRSKTPSPELDYRTPAGLHRIRAKHLTSTMDNDEPGAPPYSLED
                     VPYVMYFKGAYAFHSAFWHDRFGRPRSHGCVNLAPRDAKWLFNWAGPDLPETWHGGSA
                     TEDNPGTWVYVHGETP"
     gene            complement(22509..23522)
                     /gene="dctP"
                     /locus_tag="VFG22_08560"
     CDS             complement(22509..23522)
                     /gene="dctP"
                     /locus_tag="VFG22_08560"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HET6416336.1"
                     /translation="MRKSIVCVAAFFAISFLFAGPAPASAQEYVLEMGTVVPAGSPWA
                     LQLKRLKKYIEEETGGRVKVKLRLGGSNERSLARRTQMGSKQGFAGSVGGLSSIVREV
                     NVLEAPYVFNTVEQADKALDDPEVLKQVRAILEKQKLVFALWGENGFRSYFSRRPIRV
                     PDDMKGIRYRSQEAIAHVEAYRALGASPVTIDVANTMTSLQTGVVDGFDNTPLFAMAT
                     TWYQGLEDGDRNLILSQHSYQPGIVVYSKKWFDTLPADIQKVLLTVPYDLTTWGREQV
                     RKMEPVLLKNLVRYGYDIHDPSPEELQKFKDKEKGVPEAVAKQVGPDGQKLLKAVRAA
                     LAK"
     gene            complement(23586..24497)
                     /locus_tag="VFG22_08565"
     CDS             complement(23586..24497)
                     /locus_tag="VFG22_08565"
                     /inference="COORDINATES: protein motif:HMM:NF028121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HET6416337.1"
                     /translation="MMSSLKTGHWVALVCMVALAAGCGSTRETAWDTTSGSVQLDDAA
                     RTQVAQLMATAEAAWANRDDPAQAKAAIDAWTKASEIDPKNVKALDELTHAIYFYSDC
                     HLRFDEENPEAYKNMHETGTRAAERALSARSPAFAEKMAAGERIEDAISVLNADAVPA
                     LYWRSSNLGRWATLESFATLLSYKDEIRAIMEFCLDEDPLYFYQGPDRYFGVFYAKAP
                     SFAGGDMKKSAAHFNVAISAHPNYYGTRILMAEEWAVKEDNRPLFEELVNYVLKGDPN
                     AIPELVPEALCEKRKAAKLMSEIDDIF"
     gene            24711..25211
                     /gene="rimP"
                     /locus_tag="VFG22_08570"
     CDS             24711..25211
                     /gene="rimP"
                     /locus_tag="VFG22_08570"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HET6416338.1"
                     /translation="MSQATEHLVQALERLIDPICRAHGVELVDVRYLREPKGAVIRIV
                     IDREVPGLEIGSGVSLEDCTGVSRDVSTSLDVHEDLLPSGAFRLEVSSPGLERPLVKL
                     SDFERFAGREVKLRTQTPIRDQRRFRGKLVRVVEQSIELEQDGEVLLIPHEEIAQANL
                     VYRFSN"
     gene            25220..26992
                     /gene="nusA"
                     /locus_tag="VFG22_08575"
     CDS             25220..26992
                     /gene="nusA"
                     /locus_tag="VFG22_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HET6416339.1"
                     /translation="MAMQQSALSLQHVIEQVSQEKGIDAKILVEATEQAILTAAKKTF
                     GPDRELEARFNNETGAVDLFQYMTVVRSVENSEQEISVEEAEAHGLEAEIGEELGFQI
                     FYLPEDRDKAREQDKQFGELLSLEQSRSRFGRIAAQTAKQVIIQRVRDAERERVYAEY
                     KSRQGEIITGVVRRFERGSNIIVDLGRGVEAVLPPREQTPRESYRPGDRIVALLKDID
                     REARGPQIILSRTDVGLLVKLFEMEVPEIYEGIVRIVAAAREPGARSKIAVSSRDSDV
                     DPVGACVGMKGSRVQAVVQELRGEKIDIVPWDRDPARFVCNAIAPAEVIRVVIDEGNS
                     NMELVVPDPKLSLAIGRRGQNVRLASQLTGWRLDIVSESRFKQIEEEAMAALARLSTD
                     EELSRSLYRMGFRSLDEVVDASEGELASVPGVSADEVARLRSTAADALEELRKERLAA
                     MAELVEAPSERDKLLLVRGVNARVADLLEQNGYTSVAVIAEEEDTDRLAIKSGIGASK
                     AQALKLAVAAFISTEWPSVEAKMLASIAAAEAKAAEVARQEAEAQAAEAATAEAAEAA
                     AVADGEPDIESSIESTVSVETTQE"
     gene            26999..27607
                     /locus_tag="VFG22_08580"
     CDS             26999..27607
                     /locus_tag="VFG22_08580"
                     /inference="COORDINATES: protein motif:HMM:NF016204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF448 domain-containing protein"
                     /protein_id="HET6416340.1"
                     /translation="MLAVMGKTERDKPSRRMCAGCGARGERDDLVRLVVGEDAPYVAV
                     DLGRRLGGRGVSVHPTKPCIRSACQRGGLARALRGVAQVEPESIEAMIVAQFERRMRG
                     LLSSAQRARAVSLGTDAVRAALQANRGDLLIRAKDSRGRADELARSATAIACATATWG
                     TKASLGDAFDRSELGVLLVTDRGIARAILDCLTRVEALTEDG"
     gene            27604..30111
                     /gene="infB"
                     /locus_tag="VFG22_08585"
     CDS             27604..30111
                     /gene="infB"
                     /locus_tag="VFG22_08585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HET6416341.1"
                     /translation="MSKVRVYEVARELGVENRDLIQRIATLGIQVRNHMSVLDPIEVD
                     RIKRSLGKDRSEAMVEERIRPTVVRRKRRKPAETAEPAPAETTATPVPVDLPRAEPVA
                     ASPSPEPVFEPEPQPVEAQAPAQAPAAAPESDSPREPMAAEPGPAPAPALPPEEQGRF
                     SHDALPPGVMRRGKAKAPSATPLSEGARRRIVAEHAAQREQHAPRRREIRGRSSIGPT
                     GRPQGRPGKKRLQPGKKPKQTEITVPSAQKRVIRIEDNVQLQTLAQRMSLKATDVLMK
                     LMELGVSGVHINSTLDADTASVLANEFNYEVENVARSEDEIVGDARGEFEDKAGDRQH
                     RPPVVTVMGHVDHGKTSLLDKIRASDVVAGEAGGITQHIGAYRVDTDRGTVVFLDTPG
                     HEAFTAMRARGAQATDIVILVVAADDGVMPQTREAVNHAQAANVPIVVAVNKMDKPEA
                     NPDNVKNELAALGLQPEEWGGETIFIPCSAMTGEGVDSLLENVVLQAELLELTANSNI
                     PAEGVVLEAYLDRGRGPVANVLIRDGSLDTGDYVVAGGAWGRVRAMTDDRGRQLTSAG
                     PATPVEVLGLAELPAAGDLFYEVTDQRKAQEVAGARKKPGTSSMAPAGGMRGLDQFQA
                     LMQSGDVQELRLVVKADVQGSVEALQGALTDLATEKVKVNVIHTGVGGITENDVMLAS
                     ASQAIVLGFNVRPQGKASSTAKKEGIEIRTYSVIYEAIDEVKAAMKGLLAPTIVQADL
                     GKAEVRQTFGIPKLGTIAGCMVTEGKINRGARARLVRDGVVVWEGPMGSLRRFKEDTK
                     EVQAGMECGIGLQGFNDIKEHDIIECYEEKQVEATLE"
     gene            30135..30446
                     /locus_tag="VFG22_08590"
     CDS             30135..30446
                     /locus_tag="VFG22_08590"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HET6416342.1"
                     /translation="MVVGVCQISLSLPGVSSLKAKRSIVRKVLDRTANRFNVAVAEVG
                     QLDVHGQSTLGFAVVSNDRRHANSMLDSIAEFVEGSADAVVLNRSTELLSLGELTDWT
                     G"
     gene            30450..30839
                     /gene="rbfA"
                     /locus_tag="VFG22_08595"
     CDS             30450..30839
                     /gene="rbfA"
                     /locus_tag="VFG22_08595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HET6416343.1"
                     /translation="MVQKSFRRADRVSERIRSELMELLIRGAMRDPAAKDVVISAVRM
                     TDDLSIARVYVRVLGEIGAEQQDAVVDALKRGSGFLRREIGQRLQLRHVPSLEFYWDE
                     VVDSAIRIESILDEIRETPSSGPEGSK"
     gene            30836..31816
                     /locus_tag="VFG22_08600"
     CDS             30836..31816
                     /locus_tag="VFG22_08600"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HET6416344.1"
                     /translation="MIEEVLEIARDGERFLVACHRRPDADSLGSALGLIKCLRAVGKE
                     ATLFMPEAVPDSLLFLMDDEKGIAAPPQGARFDATFVMDIAAKALLPPGFPKPEVTGP
                     VVIIDHHHAHDDVGDIVFRDTDAAATGEVVVQLMEALGLESLPEGAATPLYAAIVSDT
                     GGFRYATTRPATLRLGARLVEAGANPWQVAYQLFERWEPARLRLLSAIIATLEVQFDG
                     RVALMRVTRQMLEICGANDDMVEGMVNYARRVEGVEIGALLWEWPVRDADGEHLETKI
                     SLRSSGTADVSVIAAALRGGGHRAAAATQLNITMDEAERRLHAELTEYLG"
     gene            31813..32724
                     /gene="truB"
                     /locus_tag="VFG22_08605"
     CDS             31813..32724
                     /gene="truB"
                     /locus_tag="VFG22_08605"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HET6416345.1"
                     /translation="MNGILIVDKPAGCTSHDVVQRVRKALRQRSVGHAGTLDPLATGV
                     LVVAIGEATKLVSHLQADDKRYEVTIALGVETDSLDADGEITRTLAVPPLDRAKVEQA
                     LRGFIGRHPQEAPKLSAIKLGGTPLHRRIRRGEDVSAPVREVELYEAKVEEVSGDAIR
                     LSLHCAKGFYVRSLARDLALELGTLGHVRALRRTASGGFSIEQALHGDGMTADAIAER
                     LIPLREACRSLRPVELTDEGVAHARHGRPIAPNLVRGDAWQQASAEESLALFAPDGEP
                     VALGRRNDEGIRVVRGFTVPSPDVVSP"
     gene            32726..>33595
                     /locus_tag="VFG22_08610"
     CDS             32726..>33595
                     /locus_tag="VFG22_08610"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6416346.1"
                     /translation="MERTRYLVVGAGVTGLAFADWLRDEDCLILEANEEIGGYCRTIK
                     RDGFIWDYSGHFFHFRHPEIERYLVERMGAQAVRRISRESRIWYGDRFIDFPFQRNIR
                     QLSEADYQDCLRGLRERTKEEGGNFKDMLLARYGRGIAEKFLIPYNEKLYALNLSALD
                     ADAMGRFFPHVSAEQILDEALAPSADGYNATFTYPEGGAIQYVQALARGIRPEQISLN
                     ERLLSLDLEQKVATTTRRQIRYDSLISSAPLVRLLEICGLEYDASILSHNKVQVFNLG
                     FDSKGPADVHWIYY"
BASE COUNT         6395 a        10204 c        10977 g         6019 t
ORIGIN      
        1 cgtgggtgcg cctcttgatt cgccgggcca tcagcacggc ctctgcggcc ccgctggcgc
       61 cgtcgtacat cgaggcgttg gcgacgtcgg tgccgaagag ctcgcaaacc accgtttgga
      121 actcgaaaat cgcttggagc gttccctgcg aaagctcggc ctgatacggg gtgtaggccg
      181 tgtagaactc gcttctctgc aggagctggt cgaccgccgg cgggacatgg tgccgataca
      241 tccctccgcc gagaaacgac agcatgttgg cgcccgtgct cgcctgcgcc agcgcggcga
      301 ggtgttgcat gagtcggggc tcgtcgagag cgggctcgag gtccagctcg cggtccaaac
      361 ggtgcgacgc gggaatcgca gcgaagagat cgtcgatgtg ctctacgccc acgcgcgtga
      421 gcatggctcg aatttcttgg tccgtgtgcg gaaggtatct cacgttcgta ccggtcgctc
      481 agctgtcgag ggttccgagg tattcctcgt acccggccgc atccatgagc ccttggagct
      541 cgccttggtc ggagggacga atcatgatca accagcctcc ctcgtagggc ccttcgttga
      601 cgagctccgg gctagactcg agcgctccat tgacttccac gacctctcca ctgaccggtg
      661 cgaagagctc gctgacggtc ttcacggact cgatatcccc gaagcgctca tgcgcttgaa
      721 cgtcctcgcc cggtgctggc atgtcgacca aggtcacatc gccgagttgc tcgacggcgt
      781 acgacgtaat ccccacccga atgaggtcgc cttcgatgcg ggcccactcg tgctcgcgcg
      841 tgtactgtag gtcccttggg tacgtcgcca ttgtcacctc ttataaaacg gggtcgtcac
      901 taccacggcg tctatgacct tgccacggat ctcgatcccc aggttggtcc ctaccttggc
      961 tagcgccagg ggcacatagc cgagaccgat gttccgtcca accgtcgggc cgggcgagcc
     1021 actggtaacc tctccaatcc gctctccatc ggccacgatc gggtagccat ggcgggcaat
     1081 tccgcgtccc gtcatctcga acccgaccaa ccttctttgg agacctgcag ccttgatgcc
     1141 acggagcgcg tcgcggccga tgaagtcgcc ggcatcgagc ttcaccaccc atccgagccc
     1201 cgcctcgaag ggatttgtct cctcgctgat gtcgttcccg tagagcatga gcttggcttc
     1261 gagacgaagt gtgtcgcgcg cgccgaggcc tacgggttcc aatccatggg gctggcctgc
     1321 ttcgaggagg gcgcgccaaa gcttgacggc atcatcgctg gcgcagaaga gctcgaatcc
     1381 gtcctcgccg gtgtagccgg ttcgagcgac ccaaaggtcg acgccggcca gggttgcgcg
     1441 ggctacgcgg aaatagggga gcccggtcag ttccgagctc gcgccgaggg acgccatcac
     1501 cgagacggca ttcgggccct gcaacgcaag aagcgcccat gcgtccgaca cgtcattcca
     1561 gggcgtttga aggctcaggt ttttggagaa gtgagtgacg atctttccgc ggttcgatgc
     1621 gttgcagacc accagcacct tttcgtcctc caatcggtaa acgatgaggt cgtcgaggat
     1681 gaaccccgct tcgttgcaac aagccgtgta cagcgcagag ccgaccgcga gcttcgagac
     1741 atcattcgtt accaagcgat cgacttcgga aagcgcacgg ggaccttcga cgaggatttc
     1801 tcccatgtgc gatacgtcga agagacctgc tcgactccgt acggcgtcgt gctctttctt
     1861 gatgcccgcg tactggacgg gcatgagcca gccggcgaag ggaacgagac gggctccaag
     1921 cgcacggtgc tcgtcggtga gcgatgtttc gcgaagtgat tcggtcaagg tgcgcagagc
     1981 ttagggacag cgggcgctta ggcaaccgct gaacgcttgg gtcgataaat cctcgatggt
     2041 ccaggacgat atcctctggc tgtgccgcgg ggggcggcgc attcgttcat tccgggttgg
     2101 cgctggcgcg atcgaggtcg gctcgcatcc cggctgcgac atcgtcgttc atgccgctgc
     2161 cgtgccggct cgtgcgctgc tttttcgagc tcatgggggc acggtccagg tctatgacct
     2221 ggccaagcac ccgggcttgg gttcgcctgc gctgatgccg atcgggggac aactatcggt
     2281 cggagctggc tattcggtgc tcagagtcaa gggcgagaag gtcgagccga ggtcggtcag
     2341 tacggagatt ctggggcggc accaatcgga atcgcgacgg ctttctctgc tcagcgggca
     2401 gggtgcgggt gcgcgagcct accacttggg cgacgagccg ctgtcgatcg ggtgcgcacc
     2461 cgacaatgcc atcagcctgc ctgatcgagc ggtcagccgg caccattgcc gcctcgagcc
     2521 ctcgacggga ggtgtgtccg ttcgggatct cggcagcacg aacggcacgt gggtcgacgg
     2581 gcttcgcatc gggcaccagg cgcttcgacc tggagcgttg cttcgcatcg ggcggaccga
     2641 gcttcgtgtg gtcgggagaa gcgggggccg tgacgcattc ggggatctgg tcgtcgagtc
     2701 gaatctgatg ttgtcggtca tggcccaagt cgagcgattg gcagccttgc cctggccggt
     2761 cctggtctgc ggggaaacag gggtcggaaa ggagcacgtc gccagggcgc tgcacgaacg
     2821 agggccgagg cgcaaaggtc cattcgtgcc tctcaacgga ggaggcctcc cgcgcgagct
     2881 catcgagagc gagctcttcg gtcacgaaaa gggagccttc acgggcgcgg ttcgagcgcg
     2941 gcgcggagcg tttgaagaag cccacggcgg aaccctcttt ctggacgaag tcgccgagct
     3001 gccagccgat ctgcagaccc gtctgcttcg agtgctcgaa acctggcacg tgcggcgcgt
     3061 cggcggcgaa tcggctcgcc gcgtcgacgt tcgattggtt tgcgcaaccc atcgcgacct
     3121 gcgcgccatg gtccacgagg gtcgcttccg tcccgacctt tactatcgaa tccaccgtct
     3181 ggtcgtggag gttccaccgc tccgcgcccg ggcgggggac atcgcgccac tggcgagaca
     3241 tttcatccgt cggatggagt ccgagctcgg cgaacggcag ctggacccgc atgcgctcga
     3301 acgccttcgg cgataccctt ggccgggcaa cgtgcgggaa cttcgcaacg tgctcgagct
     3361 cgccgccgtg cactgtccga gcgtcgtcct cgacctcgag gcgatcgatc gcgccctgca
     3421 gcgctgcgca gagccgtccg tcctgagacc gtcgggagat tcgttgcgcg aggctctgga
     3481 gcactacggt ggaaacatgt cggccgcggc gcgggcgctc ggcatcccga ggtcgacact
     3541 tcgtgaccgt ctcaggagcc cggcagagtc ggaaggctaa gcgttccgag cgaacgattc
     3601 tttggcgcgg cgaatccgca gctcgaaaag atcttgagca accccggcga ttcgcttgag
     3661 ccccgtggac ttcgagtgac cggctcgcct cggcctgcat tggatcgtga cgacgctatt
     3721 cgggacggcc gcgcccagca tccgaatcgg gaactcgaag ttcagaaaga aagtatcggg
     3781 cacgagttgg tcggcgtcga agagcggacg gcggaagagg taggggccgt cgctctgcat
     3841 gcgaacccca tgaaccaaga agatcaaacc acgcacgcct gcagaaagaa tcttccgatg
     3901 aacgccgtcg ttccggtggt catagacact gaagacgacc tcggattgat ctcgcgccgc
     3961 agcggcgcga agggttccga tcgcttcggg cgcgatctgt ccgtcagccg gcagaaacgt
     4021 gacccaagca cccttggctg cgcgcacacc cgtcttgagg gccgcgccga tgccgcggtt
     4081 ggtctcgtgg cgcagtagct ggtgcggagc gccgctcaaa gcccgggcgg cctcggcaga
     4141 tgtgccatcg gatgatccgt cgtcgacgaa gacgatctct gcagatgttt cgttggcgcc
     4201 gagccattct cggagctcga gtagcacggg tgcaatgttg gcctcttcgt tgaatgcgaa
     4261 gatgaccacg cttaggtcca gcccgttcat tccggccgtt ccctggtgac cctggctgga
     4321 acacccaccg ccaccgcgta gggtggaaca tcttcggtca ccactgcgcc agctccaatt
     4381 tgagcgcctc gaccaatccg aactccggga agaaccgttg ctcccacgcc gaggtcacaa
     4441 tcgtcttcga tgacgacgcc tgcgagctcg atcggcgagc tcagaatcgg aacgtcgcgt
     4501 ccagcctcgc catgaaagga cgtgatgata cggacgccgg gtccgatgcc gacgcgcgcg
     4561 ccgatgtcga tcccgccggc gctatgcaga aatgcctgct ggccgatcca ggtttcgtcg
     4621 ccaatgcgca tggtgttctt gtggtagccc ttgagaatcg cgtgatgccc aacgtagacg
     4681 ttcgtgccga gctcgattcg ctcggggtga aataccaaga cgcccgcttc gaagacgcag
     4741 tcagcgccgc agcgcgcgaa ctggtcggcg cgaaaagagc cgtcgccgtg actgcggtgg
     4801 cgcatctttc agagattagc aggagcggcc ggtcgagacg caaacttcgg ctgaacgggt
     4861 cccatcgcag cagagaggcc ggcgaaatag atgccgagtc ctgtcgctcc cgtctcatgg
     4921 gcaatgatag aggcgtagtg caggttctgc gcagggtgtc cgagcgcgac tgggggatcg
     4981 tatgacggaa ccggtcgaat caaccgaggg attcgctcgg gcaccgagtc gaggttcggc
     5041 tcttcgatgc cgtcagtgcg agggacgccg ctcagaagtg tcgcgattgc cgcgcgcggg
     5101 aagccttggg agtagaggtc atggccgcgg tcgagcgtga tcatggcgat caagccgtcg
     5161 gttcccggcg aacccagccc gagctccgca agcgctgccc gtgcatagcc tccgaggctc
     5221 cgctcgtggg agtcgggggc gcatcccgtt cgaacgtcca gcatccagtc gctagggcag
     5281 gtgggcaggc cgagctggtc tgccagtgcg acgctttcga tctgcaccgg atgcccaggg
     5341 ttcgcccgca agaaacgcaa aacccgcgca tcgcgtcctc ctgggccacg aagcgtgatc
     5401 aaggcgaggt ccgggtggcg tcgatacaga tgatcccaat accatccaga tgcggagagt
     5461 ccgtacgcga gcaaggtgat gtctggccga agacccgtct gctcttgaag gtaccacatt
     5521 ggaccgatcc aatgatcgtc gcgcacgatg acgatggcgt ctcgaggcgc agctgcgagc
     5581 gcttcgcgcg cgatgtctgc cgtgaacttg tcgcgatgtc gcgtgcgatg tgcggggccg
     5641 ggtggtgcgg ttagcaccag taagagaact gcagcgaggc tggccacggc aaactggggt
     5701 ttgcgagcgc cgaggtaggc aacgaggaga ccgacacccg aggcggcgat ccaggcaggg
     5761 atggcaaggt atccgttgta gtcgagaaca tcggtggcaa agacaccgtt gcgcgcgatg
     5821 aatgcagcga agaagaggag cgtcgcgttg aaaaagaatc ggccgagccc acgccttcgc
     5881 gcgtagatcg cgtagccggc gaaacccgtc agcagcaacg cgaacaggcc gttctgaagc
     5941 gaccagaaga acagctgctt catgtgatcc caccactcag cccatgaggt cacacccttg
     6001 gcactgaaat cggcggcggt gaagtagtgt ttgatcgcag cggcgtccgt tggtgcgccc
     6061 caaaccacga catcttcccg gcctgcaacg agaaatacgt aggcgtatgt tgcgagcccg
     6121 agcagtcccc cgccgatcag cagagcgagc gcgcgccgcg gaatctcttt gcgaaaaacg
     6181 gcgatcaaca atctcggtgt aatcgcgatg gctccgccaa aggcgcaaac cacgtttgca
     6241 cttgcggcga gtccaaaggc gagccctacg gagaaataga gtcgcgacct ctgatcttgg
     6301 tccagcaatg cgtttgagca tcgagccgct gcccagagca cgaagaagac cgccagtgaa
     6361 tacacctcga ttcgggtcga aggctcccaa agcgcggtgt ggacccctgc gagcgccacg
     6421 gcgggagaga tgaatcggag gtccccgggc ggacagcgag gatcagggcg caccagggtc
     6481 tgtgcgaggc tggttgcagg aatgaccgta agggctccag cgagcgcgga aagaccgttc
     6541 aatgcgatca agggatcgag gccgggcaag cgcacggcaa gcgcgccgag gagcgtatgg
     6601 agcggctgtc caaaaggatg accgagcccg agctgctccg cgacgagggc gagctcgggg
     6661 ctgtcgtaca tcgagaagct ccggcacatc gtggcgacgt agtaggcgaa gagggcagcc
     6721 gtagccgcgg ccgtggcgcg gcgcgactgg actgggaggc ccaggattcg gtcagtcacc
     6781 gtctcgacct ggcagcggca cgaagaggtt gatcacgccg cccacacctg cgctaaacaa
     6841 ctgcgtggcc cagaagccaa gcgaagctgc ataggcgctg gcctcgggaa cacccaccgt
     6901 tccgtagagc gcggcgaagg cagcctctcg gaccccggcg ccaccgaccg tgaatggaaa
     6961 gaacgcggac gcgctgatca agggcatcac cacggccgat tcggcgaact gcacggacgg
     7021 ttcgagagag tgcatgatcg catggccagc cacgacgttc acgaagtgaa tcagcaaaga
     7081 caagacgatg gcgcctagaa acggccagaa agaatagagg cgcggaagcg cccgcaaagg
     7141 cttgccgagc ttctccggga gatgaggagc gaggcgtggt gcaaccgcga tgcctagcat
     7201 cgcaaaggcc gctccggcga ggcctaggcc ggcccataag ccgacgtttt cgatgcctgc
     7261 actcgggtgc agaagatatg ctccagcggc gaggagcagg agcgccgaga cgccgatgac
     7321 gcgctcgatg aagacgaccg ctacaccgct ggtcgcgctc gcccagtcga tgtcaccgaa
     7381 ggccttgcga ccggctacac cgcgcaagat gtcgccgccc attccaccag gcgcgtaggt
     7441 gttgtagaag tggccgacga agtagaggtg agcgatgcgc ttccaagaag ggatgtcgat
     7501 cgccccgtat gcacggagca ggagcttcca tcgaatcatt ccgaagaaga gcccgaggaa
     7561 cacgacgctg accgcggtca acgcggtgcc cggtgagagt cgttcgaagg ctttcaggac
     7621 atcgcgcgga tggactcggc tcgcgagata ggcaaccgcg gcgatcgtga cggcgatgcg
     7681 cccccacctg aggtagcgcc gccgccgctc gccgctcgag ttcattcgtt ccccgttgtc
     7741 gtgcatctca gtgctcgaac cctagcgcgt tccggagctc gacgagcagt tctgaggcac
     7801 cgcattccac cctttcacga agggagggca gaaaggccgc gacgctggaa gagtgctctt
     7861 cgagcaactc caaggcgggc cgccgccgca gtgcatcgga aagcctagct aggacgaaat
     7921 ccggctcggc gtaatcccgc ggggcacccc aaatcgcgag gcgacggtgc aacgcggaga
     7981 acgcctgccc ttcgtcttcc cagcgcaagg ccccgtcggc ctcgaccgcg agtgcggcca
     8041 ggtagtcgtc cacgtgcgtg cggtcccggg tcagccatcc gtccaagaac aactcgcttc
     8101 caatgtgacc gaccgcacgg gcagttcccc gtcgcacccc acgaagagtc agctcgtcca
     8161 gcgcatagga gcagaaggcc aggaagctcg gcgtgcgatg gaatgcttcg tcggtttggt
     8221 ggtgcagctc gatcccgcac cgtatctgcg cgtcgcgaac ttcgaccagt cgaatgccga
     8281 gcatgccttc gaagtcgggt aacatactgc caagcaaatg cccggggcgc ttgtctgccc
     8341 agccggcgac gacggcatgt ccgaagaagt tcaacgtttc agttccttcg cgatccgctc
     8401 gaggccttcc ggacttcgaa gctcatcgct ctcgcagcgc cagcttacac cgggctcgcc
     8461 cttgaaccag gttcgttgcc ggcgagcgta gactcgcgtc gacttccgaa ccctgcgcag
     8521 cgtctcgtcg agcgaaattc catcacgcag atgggccaga acatcgcgat atccgacact
     8581 tccaagagga cggacctggt cgccccagcg ctctcgaagc gcacgcacct cgtcgaccca
     8641 tccggctccc agcatctgct ccgcccgcct ttcgatgagc gcctcgtgta ggctccggtc
     8701 catttccaga actacgaaca tcgcctgata gcgcggagca ccgagcgcgt gcgctgcacg
     8761 aagagctccg agggctgtgc cggtctgctc gtaaacctcg agcgcgcgca cgatgcggag
     8821 cgcgtcgttt ggatggatca gctcagcggc gagcgggtcc acctccgtca acttggcgtg
     8881 tagggcggct gcgccttcgt ttcgcgctgc ggtttcgagc cgactgcgca gcgctgggtc
     8941 gaccgggggc accgcgacga gccctcgcac gagcgcacga atccagagcc cggtgccgcc
     9001 gacgatgatc ggaaggtgtc cgcggccgcg aatctcacct atcgcagtgt ctgcgagctt
     9061 ggcaaacgtc atcgcgtcga cgtcctgatc ggggtcgaag acatcgatca ggtgatgcct
     9121 cacgtcgccc agctcagatg cggtgggttt cgccgccccg atgtcgaagc cacggtagac
     9181 ttgcacgctg tccgctccca ccaactcgcc gccgaagtgg cgtgcaagct cgattgcgac
     9241 gctggtcttg ccggtgcccg ttgcacccgc gacgacgaga atgggccaga gcggttcagt
     9301 catcggcgat tccggcggct ggcgatggaa gtgtaactcg cgcgaggatg caggcgcggt
     9361 gccgatcgtc gctgagccgc acgttggcaa cgatcgcttg cacgccgcgt tcgtccagcg
     9421 actcgccgtt gggcgtggcg gccgttttcg ccagggctag cagggtggtc ccgaacacgg
     9481 gctcagcgcc ggaccccaag cttgcaatcg cgcccttgaa cagcgcgggg gggcgagctt
     9541 ccgccagcag cgccggaatt gctctcagca cgtaggatcg ttcgccgagc gcggaccagt
     9601 caaatccgag ggaacgaagg atcgcttcat gcctttccaa ggatgattgc tccgatccgt
     9661 tcagatcgag gcggtccgga aacaacagat tcgatcgagg cagcgtgccg gcctctgaag
     9721 cctgccgaag cgcatcgtat cgcacctcgg tctccgctcg ggcgcgatcg agcacgacga
     9781 gctcctcctt cgattcgcag atcaagatgc ggtccctcac ctgggcgacg aaacggaggc
     9841 cgttcgaata gcgcgggcgg aatacctcga tcggttcggc caaggtcgaa gggggtgccg
     9901 atccgggagc ttccgtcgcc cggccctcga tcgggccatg cgcaccgata cgcgcctccc
     9961 aataggcatc gcccggccga tgagccggaa gccgcgcggc gatcatccga gttacgcggt
    10021 ccaccaattg cgccgtgttt ctgaagcgca cctcggtctt ctgaggatgc gcattcacgt
    10081 cgacgtcttc cggtgcgagg cggagtgaga ccaggccgcg cggatactgt ccgggtggca
    10141 gccggtcgcc gaaagcaaac gcaactgcac gggccagccc gcggtcgttg attgggcgcc
    10201 cgttgacgaa gagatagagg tggcgcgccc cggcgcgggc gtactcgacc ggcgctagca
    10261 gcgcatccag caggaccccg tctcgttccg cgtgaatctc ttgcagcgcg acctctccaa
    10321 agacctggta tgcgcgttcg gcgaggctcc gtgccgggag gtattgcctc gcggtgcgcc
    10381 catcggatgt cacttgcaat cgcagctccg ggcgaccgag ggccacgcgc tgacaaacct
    10441 cgaatatgcg cgaggtttcg gtctgacggg cccgaagaaa cttctttctt gctggcacgt
    10501 tgtagaagag gtcttggact tcgaccgtcg tcccaggtgg gcatccgctg ggagatatct
    10561 cgcgcagatc gccgccttcg accaggactc tggttcccgc caggtcctcc ttgggtcggg
    10621 tcgtgatcgt catcttcgag acagccgcga tggagggcag ggcctcgccg cgaaaaccga
    10681 gcgatcggac ctggaccagg tcctcgaacg aggcgatctt actggtcgcg tggcgttgga
    10741 tgcagagctc ggcgtcctcg ggcgacatgc caattccgtc gtcggtgacg cgtacctggc
    10801 ctcttccccc gtcttccact gacacggtaa tggcgcccgc cctggcatcg atggcgttct
    10861 cgaggagctc tttcacgaga ttggctgggc gctcgatgac ctcgccagca gcgatctggt
    10921 cgatcagcgt tggaccgagc aatcttacgg ttgggctcac cgcctgcgcc tatcaaagct
    10981 ggtttcgctt ggcaacgtgg gccggcaatt ccggggactt cgttgctccc ctcacaattg
    11041 ctctgctacc gtgatttcgg ggtctcaaac agcaatttgc catcatgaag gtttgtccgg
    11101 cgtgcagagt tgactacggc gggggagagg tattctgccc cgtcgacgcg acgcgtctcg
    11161 tcacgacgtc gcagatgacc gtcaatccgc tcaatcctga cgatccgctg attggaagag
    11221 tgctcgccgg caaatatgtc gtgaagcgcc ggatcggcga aggggggatg ggcctggtct
    11281 acgagggcat tcaccgcgac atcgacaagc aggttgcgat caaggtcctt cgggacgacc
    11341 tctcgcgacg gcccgaggtg gtggcgcggt ttcgacggga ggcgaggagt gcatcgcgga
    11401 ttgggcacga gcacattgtc gatatctccg acttcggtga aaccacgcgc ggcgccagtt
    11461 atttcatcat ggagttcctc gacggggaag acctcggaaa cgttctcgga agagagggca
    11521 ccgtcgatgc agagcgcgct tgcaagatcg ttctccagtg ttgccgtgcg ctttccgcca
    11581 cgcatgacaa ggggatcgtc caccgcgaca tcaagcccga gaacatcttc ctcacgacac
    11641 gcgatggaag gaacgacttc gtcaagatcg tcgacttcgg catcgccaag atgagtgacg
    11701 tcgagaccga tggcgcacca gggcgaaagc tcaccaagac cgggatgatc ttcgggacac
    11761 cggaatacat gtcacccgag caggccgcgg gcaaagagct cgaccatcgg gtcgacgtct
    11821 atgcgctcgg catcattctt tacgagtgct tggccggccg tgttccattc gaaggggata
    11881 ccttcatggg agtgctgact cagcacctgt tcgccgaggt gcctccgatt gcggaattga
    11941 actcgaatgc caaaccgagc cgcgagctcg agctggtgat ccgcaagtcg ctcgcaaagg
    12001 atcccgacga ccggtaccag gacacgaacg agctcgctga ggcgatcgag tgcgctctcg
    12061 acggccgatt gagccgcgcg acggtgcgga ctccgccgtc cgcattcggg cccccgtcgc
    12121 tcgcgtatct cgatggggtg ccccgccgca aggcgtcgcg ttgggtctgg gcgttgagta
    12181 tcctgggtgc tctgggcgcg attggctggg ttgcactggg gcctgcttcc gggccagcga
    12241 gtgaagcggg cgctagtgat gtgccggctc aggcgatgcg cgccccaatc gagcgggttc
    12301 ccgatttgga ggaatcgcct tcagcggacg tcgagtcggc ggagacgctc gacgaaccgg
    12361 ttctcgttcg cgttcatgtg acgaccgatc caaccggcgc cgcggtcctg ctcgaggatg
    12421 gctcgaaggc ttgctcgccg acaccctgca ctgtcgaggc agcgccgggg gcgtccttgg
    12481 tgcttcgcgc gaagcttggt agacggaggg ggcagaccgc gattacccca accgacgagg
    12541 aaaccgtttt gatccagctc gaagtcccga agagggcgaa gccaagtccc cgcagcgcgg
    12601 cggcccctcg tcgagagcgc gagcgaccgg tccggtcctc ggatctcaag gttcccgagt
    12661 gggcacagtg agtgaggtcg agcgcaagac gctcgccgac ctcgagtggg agcaggtgga
    12721 gtgggcggtg gccgaacggt gccgaggacc gcttgccaac ggcctgaagc tgcctctcgc
    12781 cgagtcgttc gccgagaccg agcgcgcgct cgccgaggcc aaggaggctt tttcgcttct
    12841 tacccggaac gaaccgctgc ctctcgaggg gatttgcgaa atcggggttt ccttgcagca
    12901 cctcgagcgc gaaggcgtcc tagacggaag cgctctcaag gatatccgaa cgaccctgcg
    12961 tgcggccgcc gagctgcgcc ggtttctcgg tcaacgaaaa gagagcgtgc cgtatctgca
    13021 ccgcgcctgc gcaatcgatc cgaccctcga gagtctcgag gaagagcttg gcatgtcgat
    13081 cggagacgac ggctcgatcc tagactccgc aagcgcagag cttgcacgcc ttcgtgagga
    13141 ggcaggcaac ttgcgggccc gaatcatccg caagctcgaa gacctgattc agaagcgaag
    13201 cgcaatcctc caggactcgt tctacacgat ccgcgaggga cgctacgtac ttcccgtgcg
    13261 ggccgacgcg catgaacggt tccacggcat cgtgcacggc tcgagcgcga gcggcgcgac
    13321 ggtcttcgtc gaaccgagag agctcgtcga gcgcggcaac cgcctgaaga tggtgcaggc
    13381 cgagctcgaa cgggaagagc gcagaatcct cgccgcgctc gccgacctgg ttcgccagta
    13441 cgttccccag atgcgtgcag ccgccgaggc cctcgaccac gcggacctgc gcggagcgag
    13501 cgcccgtctc gccgtcgacc tcggcgccga attcatcgag ctgagcgagc agccgcgcat
    13561 ttcgctgaga ggcgcccgtc atcccctctt ggtgctgcgg gggatcgatg tcgtggcgaa
    13621 cgacctcgat ctctctggag gcgaagccct ggtgatctcg ggcccgaacg ctgggggcaa
    13681 gacggttgca ctcaagatgc tcggactcgc ggcgctcatg atccgggcag gtctccccgt
    13741 gcccgcggag gaggggagcg tctgtggctt tttttcgccc gttcttgcag ccgtgggcga
    13801 cgagcaaagt ctcgaacgaa gtctttcgac gttttctgcg cacctggtca cgctccgcgg
    13861 cgtgctcgag cttgctggcg agcgggttct cgtcctcctc gacgagctcg caggcgccac
    13921 cgatcctgaa gctggggctt cgcttgcttg cgcgatcatc gaggcattgg tcgaacgcgg
    13981 gaccgcggtt gccgtcacca cgcactacga agcgttgaag gcgcttgctg ccgagcgcga
    14041 ggacatgcgc aacgctgcag tcggcttcga tttcgacaac atggccccca ccttccgatt
    14101 ggcgatgggt gtgccgggag gctcgaacgc actcgagatc gcgaggcggt acggtattcc
    14161 gggcgagatc atcgaggctg cgcgcagcgc gcttccagaa cactcccgga gcttcgagac
    14221 gctggtggcc aagctcgagg cggcgaccaa ggccgtgctc gaggaacgca cttcgctcca
    14281 ggaaatgcgg acgaggctca gcgaggagag cagtaagctc gacgcacgcc aggcggctct
    14341 caaggcgcgg gaggcgaagg gcctgggcaa ggaggcgcaa gagctggtgg accaggtgcg
    14401 cgagacgcag cgcagactcg agcgggcgaa gaaggatctc cgtgagcgag ccgaagccga
    14461 gtcgatagcc ttggcgggcg ccacgctccg cgatgcgggt gccgtgctcg gagacctcga
    14521 cgcgcaccgt ctgagcctgg aacctgagcc tgtcgaaacg tcagcgctcg atgtcgaggc
    14581 ggttcgcgtt ggagatcgcg tctgggtaga ccggcttcgc agcgctgccg atgtcatcga
    14641 gggtccggcc cggggcaagg ttcgtgtctc tgcaggaatg atgaagctct gggtcgacct
    14701 cagcgatctt cgcgcgctca agcaaagcgg gctagctccc acgaaggacc ggacggccca
    14761 atcgggagcg cacccaaagc cacagaagag cattcgaacg tccgacaaca cgctcgatgt
    14821 ccggggcctt cgtgcggacg atgcggtcgc cctcgcggag gcgtttctcg atcaaatgta
    14881 tggcgcagcc gagccgaacg ttttcatcgt gcacggcgtc ggaagcgggg cactccgaca
    14941 tgccatccac gagcacttgg cgcgtgatgc gagctacgtt gccggtttcc gcgaggctac
    15001 tcaggacgaa gggggccctc aggtgaccgt ggtgtccttg aagtaggggt cagatttcgc
    15061 tgctggcggg tgcaccctcg gccatgcgcg gcttcacacc gagctgccag accgcgaggc
    15121 caaggcacac catcaggatc gacgaccact gcgcaggggt taggcctccg tatcgcacgt
    15181 ctccgccctc gagtggcgtg gctcggagga agtcgaggaa gaaccggacg ggtgcgtaga
    15241 gcaggggcag caagacgcag tagaacccga ctggacggcg cttgcgatgt tcgagccaaa
    15301 ggaagagcgc gatgatcgag atcgaaaaga gtacttcgta gagcccgaga tcgtgccgcg
    15361 cctcgaacgg ggggttcccg aagcggtagt cggccaccgc aagcgcgaag tccgtaacct
    15421 tgcccggatg atcgtgcacc acgaagcagc ccagtcggcc gaacaaccaa ccgaacggca
    15481 aaccgaaaca cacggcgttc gcgtacggaa gaagcggcgt cttctttcgg tacttccaaa
    15541 tgaagcatcc gagaatgccg ccgaagaaac ctccatagga cgagagcccc agccaggtcg
    15601 taaagaacat cgaggggttt cgcagaactt cgacgagggt ttcacgctcg tagaacagac
    15661 cgttcaaaaa gtaaccgagg atgaatcccg tgacgactgc gtacgtcacc aaatctccgg
    15721 tcgcgacggt gtcgagcccg tttcgttgcg caaatcgacc cgcgacccac gcgcccagaa
    15781 gcacgccggt ggcgaccagc acgccaaacg gttgaatcga caaggtatct cccagcaccg
    15841 gcagcgagaa cggcagcgga atgtcccaag cttcgagacg gaaccaagga atgaacagaa
    15901 atgcgtgcat atggcggccg agcctagcac ccacatcctt gcatcgcccc tagacagccg
    15961 ctaagggcag cgcgaggtga aatcatgacc gagatcatcg cgatcggcgc acgagaaatt
    16021 ctggactccc ggggcaatcc aaccgtcgaa gtcgacgtgg aattgatggg cggagcgagc
    16081 ggcagggccg ctgttccttc cggagcttcc accggcgaac atgaagcgct cgagcttcgt
    16141 gacggcgatc ccagcagata tctcggcaaa ggcgttctca aggccgtgga caacgtccta
    16201 accgagatcg cgcctcaggt gatcggacac gatgcgctcg atcaagcagc cgtcgatcag
    16261 gtgatgctcg actacgacgg tacgccgtcg aaggcaaagc tgggggcaaa cgcgattctg
    16321 ggcgtctcga tggccgtatc gagggcagct gccgatgcgg ttggcctgcc gctgtggcgg
    16381 tatctagggg gcgtccaggc ccaggtgctt cccacaccgc tgatgaacat catcaacggt
    16441 ggcgcccacg cggacagtgg cctcgagatt caagagttca tggtcgtgcc gcacggcttt
    16501 gatacgttct ccgatgccct ccgagctggg gcggagacgt tccacaacct caagaagctt
    16561 ctcaaggagg ccgggcactc gacgggcgtc ggtgacgagg gcggcttcgc tccacacctt
    16621 tcgaccaacg aggaagcgct tgcgttcgtc gcgcgggcga tcgaagcggc cggctatcgc
    16681 cccggagagc agatttccat cgccctcgat tgcgcggcca gcgaattcta cgacaagaag
    16741 accggggtct ataccttcga caagaagccg gtggatgcag caggtctagt cgaaacgtat
    16801 tcggactact gctcgaagta tccgatcgtg agcatcgaag acggcatgga cgagaacgat
    16861 tgggatggct ggaaagtcca gaccgacgtg cttggagccc gagttcagct cgtgggcgac
    16921 gatctcttcg tgacgaatac cgaacgcgtt cagatcggca tcgaccgagg gatcgcaaac
    16981 gcgatcctga tcaaggtcaa ccagatcggc accgtcaccg aaactctcca cacgatccga
    17041 ctcggcgcgc tgcacggcta tgcgtcgatc atctcgcatc gcagcggcga aacatccgac
    17101 acgttcatcg ccgacctcgc cgtggccacg ggtgctggtc agatcaagac cggctccgcg
    17161 tcgcgctcgg agcgaatcgc gaagtacaat cagctccttc gcatcgagga gcagctaggc
    17221 gaagcagcgg tctatgcgcg aaagtccacc ttcgcggcat gaagtgaaca tggaaggcct
    17281 cagccgacgg cgaagcgctt tgacgactcg ctgtcgtact gaccggcgcg aaacgcgtag
    17341 tcgctgagga tttttcgcaa gatgtgcacg ttcgtcgcga agggctgaac gcgcatctct
    17401 gcgagcattc gatccatcga tagaagcgct cgatggcgga taggttcgaa ggtcgtcacc
    17461 tttgcgatca aaggtcggtc gtcggccggc aggggcaggc caaccgtcgc cgaggacgtt
    17521 gcgcgaacgg atccctgggg cgccggcgac accgaatacg acgagacgac ggcttcttgc
    17581 tggtcgagcg cgagaactga tgcgtcgagc gcgtgaccgc gtggctcgat ggtttctatc
    17641 tcttccggga tcgcttcccc cgatgcgatt cgcaattgga gcgcgaccaa gtccacgccc
    17701 atgaccattt cgatcagcgt atggtgtcgc ggtaggccga ttcgcacgtc ggacacccag
    17761 aacaaacccg aagggtcgat ctgaaactga atctctagca gcccggcata ccgcagctcc
    17821 gccgcaagtc gtcgagcgct ctcgaagagc gccatccgaa acgcttcgcc gtccgagcga
    17881 aagagaagct cgggcgaagg agtctcgtgg atcagcgtgt gcccttccag agaaaggctc
    17941 ctgtcgcact cggcgatcgc ggtcgtgtta ccgtgcgaat ccgcgatgac gatcacgccg
    18001 atcgcccgcg gcctcgacgg gtccggtcga tcatgcacgg tccgaacctc ggctcggtcc
    18061 gccgccgctt ttacggatgc ctgatcggtg agcgcgccaa gaacatcgag gtccgttccg
    18121 atcaccgcca catcggcctt ctccgccgcc gccgcgagct cgaacatctc cggctgcccc
    18181 tggtaaccga ggtgcgctgc gtcggcgccg gcctgctcta gaattgcggg cagctggtcg
    18241 gttggaatcc ctcgcgcctc ttcgaagtcg acctcgatga tccggtcggc tgcttcgatg
    18301 tgtcggctcg cggtttcgtt cttgggtgcc actacgatgg attcgacgcc cagccttcgg
    18361 caggtccgcg caacgcgggt cgcggcctct ccacggcgca gaatcagtac tttctcgaac
    18421 accggacgag catagcaacc ggtccgccca aggcaccccg ccgcttggct tgacccaatc
    18481 cccaggcgga gccactagcc ccacaatgcc ggtttggctt cgttcacgag gattggaacg
    18541 ccgttccgtt cgtcactccg agcttcgatg gagagctgaa gcgatgcttg aatcgctggg
    18601 tctcggccag gcggagttgt cgattcttct ctgcgacgac gagtcgatcc gcaggttgaa
    18661 ccgccgttat cgaaagaaga accgcgccac cgacgttttg gccttcccga tgcaagaggg
    18721 cgagggaccc atcgtgcatc cgggcctcct cggcgatgtg gtgatctccc tgccgaccgc
    18781 tatcgtccag gcggccgcac acgatcgccc aatcgtcgac gaagtgacct ttttgttggc
    18841 ccatggactg cttcatctgc ttggatacga tcacgccacc aagcgcgagg agcttgaaat
    18901 gagcgcccgc acccgcgatc tgctggccgc ggtcgaggcg gccaggtagc cgtcagcgct
    18961 gtggataacc gccctgcgag gggccaaatc cgagggccga ttcttcgcgc aacgctttga
    19021 atttgacgtc taagccaccg aaaggtcgtg tgattcagca acgcgcgagt gttcttctcg
    19081 atgtgccagg cccgagcgga agagcctgtg ggatctctgt ggcaaaggcg cgcggcgcgc
    19141 ccggggttgg ccgcaggtgt tcccgcttgg gtcgccgcag attgattaac cccaagaaat
    19201 ttgttcgcga aagctcacaa agacccttgt cagcgcggaa aaccccaggc atagatggcg
    19261 ccgtgtctgt ctgaggcttc gttttggggg ccttttcttt agatcgaacg gcaggccgga
    19321 gagttaggag ggaactatga cagcgggcaa gcgaatgaca aaggcccaga tcatgagcga
    19381 gctggccgac aagagcggcc ttaccaagaa ggaaattggt gcggtcttcg atgcacttcg
    19441 agatctcgtc aaaaaagagc ttggtcgccg tggcaccggg gagttcgtca ttcccgatat
    19501 gatcaaactc aaggttcgta agattccggc aaaacctgcc cggatgggac gcaatccagc
    19561 caccggcgag gagatgatgc ttccgcccaa ggccgcgtca aagaagattc gcgcgacgcc
    19621 tctcaagaag ctcaaggacc tggtcctctg agtcactgag acaacgaatc gagaaagagg
    19681 ggccctcagg gccccttttt tgtttgcgat ccgcgtagtc ggccctcgac gtaactgagg
    19741 atgaaccgaa tttgatcggc gttgagctcg aaggaaacct cgatgcgctc gtctaccggc
    19801 ttgaagtcca ttcggcgcag ggtcttgccg aaacctgcga gactcatgat cagctgctgc
    19861 gaatagaaca tcgccacttt cctccaatag accgaggcct cctgcgcgag cctgtttgag
    19921 gggtaggtcg ccaagacgct gactgtcacg acgttgggag cgctttctcg cacggccaag
    19981 cgcagacgga tcggtacgtg cttgatgttg ccgaggatga actggtgaac accttcgatt
    20041 tcgagggaga tcacctcttc gggacccatc gagagcaacg cttcgggacc ccgtgccgcc
    20101 acgagacctt cctttttcgc gtcgcgaagc tctcgcgcct gcgccatcgc gagtacccgc
    20161 tcgagatcct gttttcgggt gatgctgaag tgcttcgggc cgagaaccgc gatcgtgcga
    20221 agtgtcttat cccggttgtg ccaaggcgcc gtcgcaactc cgtagcgtcg ttgccaacga
    20281 accgacttgc cccgggagcg cgcgagccga ttcacgcttt ggcgggcgaa tccatcctcg
    20341 cggccatgtc ggcccgcaag cacgagccgg gaccgctcca ggttgggggt ggcgacgaga
    20401 agtcggtcga ggtccgcgac cggatcgatc ccggacccgt ccagtaacaa ctgccaatca
    20461 ggcactccgc gcaagaagcg cgtcacatcg ggagccaggg tcgactgccg cacccgcttc
    20521 aaatccacgc gaagcgcgag ttgagcgcct gggggaatcc gcgaggggcc gctgagcgtc
    20581 ggtggtgcga cgacagtgtc ggtgtcagcg tcagtgtcag cgtcggtgtc agcgccggtg
    20641 tcagcgtcgg tgtcagcgcc ggtgtcggtg tcagtgtcag tgtcggtgtc aggctcagcg
    20701 tccgtgtcag ggaggacgcc ggcgtcgggg gggtggtctt ccccaccttc gacggcctcg
    20761 cgggcccagc gcgggtccgg cacgccggtg gcccgaggcg gttcggacgc tgcggccatc
    20821 tgcatctcac cggtgagccc gaactccacc tcggcgggca gcgtgaggtc gaagtcgaag
    20881 tcgtagtgag ccgtggcgct gctgatgtag gcgagggccg caatgtggag caaggtcgat
    20941 gccacgattc cgaacgccgt ggaaaacggg tcgaggtatt gaccggtgcg catcctaatg
    21001 acagttcgta gcagaacgaa gccgtgcaat gcacacctcc aactgccatg agcatccgta
    21061 gcttagtttt cgtcatggtg gccgcgtcgt cgctgccgct ggcttcaggc ggttgcgatc
    21121 tcgacgagcc attcgagctc atcgtgaaca agcgacctcc ggagcccccc gatcttgggc
    21181 cgcctcagag ggtctttgcg aagcgcttcg tgagcccaat ccggtctgct ccaagcccgg
    21241 aggctgagcg cgtgggttac ttgcgcgcag gcgcgctgct gcactccaag accagcaagc
    21301 ctcttgggta cgaggggtgt gaaggcggtt ggtacgagct cgacaccggc gggttcgctt
    21361 gcaacgcacg ggatgtcatc gttttttctg gcgagcgatt accggagctt cgggcacgcc
    21421 aagccgatcg tgatgccgtg atgccttacg aatacgtcac ggtccgcagg aacacgccgg
    21481 tctacaagcg cattcccaga gcggaggaga tctacgagat tccggtcgat gccccggatg
    21541 ccggtgccga cgcggcagaa cgcgaggcct ccgaggcgcg cggacctctc gacaacccac
    21601 tcgtagtgcg ggtacttcag ccgggctttt acgtgagcct cgatcgcagc ttcgaaagag
    21661 acggcgtcac gtattggcgc actcagcaaa acggattcct tcccggagac catctgcgtc
    21721 acaagaagtg gagcgagttc aaggggcaag agctttccgt cggcgactgg ggccttccag
    21781 ttgccgtgac gcggtccgac gaaaccaccg tttaccggat gaacgaacgg ggcaagctgc
    21841 gagccacgcg gggccggctc gacaagcgcg cttggctcca ggtgcgctcg cgccagcgca
    21901 tcggcgacgc cgccttcctg atgctgcaag acggaacctt ggtgcgcgaa acggacgtgc
    21961 tcctgattcg gccgagcgcg ccgcctgagg gcgtcccaga aggcgagcgc tggatcgatg
    22021 tcgacctaga gcaccaggtc ttggtggcct atgacggaat gcgccctcgc tatgtgacgc
    22081 tggtatcaac cgggcgaagt aagaccccga gccccgagct cgactaccgc acgcctgcgg
    22141 gcctgcacag gattcgggcc aagcatctca cttcgaccat ggacaacgac gagcccggtg
    22201 cgccgccgta ctcgctcgag gacgttccgt acgtgatgta cttcaagggc gcgtatgcgt
    22261 tccacagcgc cttctggcac gaccgattcg gccgcccaag gagtcatggc tgtgtaaatc
    22321 ttgccccccg cgatgcgaag tggctcttca actgggcagg gccggacctc ccggagacct
    22381 ggcatggagg gtcggcgaca gaggacaacc cggggacctg ggtctacgtg cacggagaga
    22441 ccccctgaag tcgatgcctg gcatcgaccc cggggcaaac ctgaaccgga aggaaaggaa
    22501 aggcgcgctt acttcgccag tgcggctctg accgctttca ggagcttctg accgtcgggt
    22561 ccgacttgct tcgctacggc ctccggaacg cccttttctt tgtccttgaa cttctggagc
    22621 tcttcaggac ttgggtcgtg aatgtcgtat ccgtagcgga ccaggttctt caagagcact
    22681 ggctccatct ttcgcacctg ttcgcggccc caggtcgtga ggtcataagg cacggtcaag
    22741 agcactttct gaatgtccgc tggcaacgtg tcgaaccact tcttcgagta gacgacgatg
    22801 ccgggctgat aggagtgctg ggagaggatg aggtttcggt cgccgtcttc taggccctgg
    22861 taccaggtcg tcgccatcgc gaagagtggg gtgttgtcga agccgtcgac cacgccggtc
    22921 tgtaagcttg tcatggtgtt tgccacgtcg atcgtgacag ggctcgctcc aagagccctg
    22981 taggcttcta catgggcgat cgcttcttgt gaccggtagc gaataccctt catgtcgtcg
    23041 ggaacgcgaa tgggccttcg gctgaagtac gaacggaagc cgttctctcc ccagagcgcg
    23101 aagacgagct tctgcttctc gaggatcgca cggacctgct tcaatacttc gggatcatcg
    23161 agcgccttgt cggcctgctc gaccgtattg aacacatagg gcgcctcgag gacgttgacc
    23221 tctcgaacga tggagctgag gccgccgacg gagccggcaa agccttgctt gctccccatc
    23281 tgagttcgcc gcgcgagcga ccgttcgttc gagccgccga ggcgcagctt gaccttcacc
    23341 cggccgccgg tctcttcttc gatgtatttc ttgagccgtt tgagctggag cgcccaaggg
    23401 ctacccgcgg ggacgaccgt ccccatctcc aagacgtatt cctgcgccga tgctggggct
    23461 ggtccagcaa acaggaagga aatagcgaaa aacgccgcaa cacagactat ggacttacgc
    23521 attggattct cctcggaggg gcgggcttgg cccagtggac gctagggtgt tcgagcctat
    23581 tcagttcaga agatgtcgtc gatctcggac atcagcttcg cggccttgcg cttttcgcag
    23641 agggcctccg gcacgagctc ggggatggca ttggggtcgc ccttgagaac gtagttgacg
    23701 agctcctcga agagcgggcg gttgtcttcc ttcacagccc actcttcggc catcaggatt
    23761 cgagtgccgt agtagttggg atgcgcgctg atcgcgacgt tgaagtgtgc cgcggatttc
    23821 ttcatgtcgc cgcctgcgaa gctcggcgcc ttcgcgtaga agactccgaa gtagcggtct
    23881 gggccctggt agaagtagag ggggtcctca tcgaggcaga actccatgat cgcgcgaatc
    23941 tcgtccttgt acgagagcaa agtcgcgaag ctctcgagcg tggcccaacg gcccaggttc
    24001 gacgagcgcc agtagagtgc aggcaccgca tcggcattga gcacgctgat cgcgtcctcg
    24061 atgcgctcgc cggccgccat cttctcggcg aaggcaggcg accttgcgga aagtgctcgc
    24121 tccgcggcgc gcgttcccgt ctcgtgcatg ttcttgtacg cttcggggtt ctcctcgtcg
    24181 aaacggaggt gacaatccga gtaaaagtag atcgcgtgcg tcagctcgtc gagcgccttc
    24241 acgttcttcg gatcgatctc tgaggctttg gtccaggcgt cgatcgctgc cttcgcttgt
    24301 gccgggtcgt cgcgattcgc ccaagcagct tccgccgtgg ccatgagctg agcgacctgc
    24361 gtgcgggccg cgtcatcgag ttgaacggat cctgaagtcg tgtcccaggc ggtttcgcgg
    24421 gtcgacccac aaccggctgc cagggcaacc atgcatacca gggcaaccca atgcccggtc
    24481 ttcaacgaac tcatcatgct ttcctcctgc tcctcgccga tcaggcgggg gtactggcgg
    24541 ggcataccaa gaggggtttc acgctgtcaa cgtcgcaaag cggttgcgcg tcggtttgct
    24601 tgcagggggt ggggcgctgt ggtacgagcc tgcctgaaaa tttggtttac ctaatcctgg
    24661 gtgggccgcc acggcccgcc cgtttttcgt ttagcgaccc tgagtagaat ttgagccagg
    24721 ctactgaaca tctcgtccag gcattggagc gactcatcga ccccatttgc cgcgcccatg
    24781 gcgtggagtt ggtcgacgtt cgctacctga gagagcccaa gggtgccgtg attcgaatcg
    24841 tcatcgaccg ggaggttccc ggtttggaaa tcgggagcgg ggtgtctttg gaggattgca
    24901 caggggtgag tcgggacgta tcgaccagtc tggacgttca tgaggatttg cttccttcgg
    24961 gggcgtttcg cctcgaggtg agctcgccgg ggctcgaacg ccctttggtg aagctctccg
    25021 atttcgaacg attcgccggt cgcgaggtca aattgcggac acaaactcca atcagggacc
    25081 agcgccgctt ccgcggcaag ctcgtcaggg tcgtcgagca gagcatcgag ctcgagcaag
    25141 acggcgaagt cttattgatt ccgcacgagg aaatcgcgca agcgaacctc gtgtatagat
    25201 tctcgaatta ggtgggtaga tggctatgca gcaaagcgcg ctctcccttc agcacgtaat
    25261 cgaacaggtc agccaggaga agggcatcga tgccaagatc ctggtcgagg ccaccgagca
    25321 ggcgattctg acggcggcca agaagacctt cggtccagat cgcgagctcg aggcgagatt
    25381 caacaacgaa actggggcgg tcgatctttt ccagtacatg accgtcgtca ggtcggtgga
    25441 aaacagcgaa caggaaatct ccgtcgaaga ggcggaggcg cacggtctag aggccgagat
    25501 tggcgaggag ctcggctttc agatcttcta cctgcccgaa gaccgcgaca aagcgcgtga
    25561 gcaggataag cagttcggtg agctcctgag cctcgagcag agcaggagcc gtttcgggcg
    25621 catcgcagcg cagaccgcga agcaggtgat cattcagcgc gtccgtgatg cggagcgcga
    25681 gcgagtgtac gcggagtaca agagccgcca gggcgagatc atcaccggag tggtgcgacg
    25741 attcgaacgc ggatcaaaca tcatcgtcga tctcggtcga ggtgtagagg cggtgctgcc
    25801 gccacgagaa caaactccgc gcgagagtta tcgcccgggg gaccgcatcg tcgccctcct
    25861 gaaggacatc gaccgcgagg cgcgcggacc gcagatcatt ctctctcgca ccgacgtcgg
    25921 cttgctcgtc aagctcttcg aaatggaagt gcccgagatc tacgagggta tcgttcgcat
    25981 cgtggcagcg gctcgcgagc caggtgcgcg ctccaaaatc gcggtgagca gccgggactc
    26041 ggatgtcgac ccagtgggtg cctgtgtagg catgaagggc tctcgcgtgc aggccgtcgt
    26101 tcaggagctt cgcggcgaga agatcgacat cgtaccctgg gatcgggacc cagcacgctt
    26161 tgtttgcaac gcgatcgcac ctgcagaggt gattcgtgtc gtcatcgacg aggggaactc
    26221 caacatggag ctggtggtcc ctgatccgaa gctttcgctc gcgattgggc gccgcggcca
    26281 gaacgtacgg ctcgcttcgc agctgaccgg ctggaggctc gacatcgtga gcgagtcacg
    26341 gttcaagcaa atcgaagaag aggcgatggc cgccctcgcg cggctcagca ccgacgagga
    26401 gctatcccgt tcgctgtacc gcatgggctt ccgaagcctc gatgaagtcg tcgatgcgag
    26461 cgagggtgag ctggcgtctg ttcccggcgt gtctgcagat gaggtcgcaa gacttcgctc
    26521 taccgccgcg gatgccctcg aggaattgcg caaggagcga ttggccgcga tggccgagtt
    26581 ggtggaggcg cccagcgagc gcgacaagct gctcttggtt cgcggtgtga acgcccgtgt
    26641 tgcggatttg ctcgagcaga acggctacac gtcggttgcc gtgatcgccg aagaagaaga
    26701 caccgatcgc ctggcgatca agtcggggat cggcgcgtcg aaagcgcaag cgctcaagct
    26761 cgcggtcgcc gcgttcatca gcacggagtg gccgagtgtc gaggccaaga tgctcgcgag
    26821 catcgcagcc gctgaagcga aagcggctga agtggcgagg caggaagccg aggcccaggc
    26881 ggccgaagcg gcaacggcag aggcggcgga ggcagccgca gtcgccgacg gcgagcccga
    26941 tatcgaaagc tcgatcgaat ccacagtctc cgtggagacc acgcaggagt aggcgccgat
    27001 gttggctgtc atgggcaaga ccgaacgaga caagccgtcg aggcggatgt gtgctggctg
    27061 cggtgctcgc ggcgagcgcg acgatctcgt tcgtctcgtc gtcggagaag acgcgccata
    27121 cgtcgctgtg gatctcggac gaaggctcgg tggtcgaggt gtctcggtcc atccgaccaa
    27181 gccctgcatc cgttcggcgt gccagcgcgg aggactcgcg cgcgctttgc gcggcgtagc
    27241 ccaggtggaa cctgagagca tcgaggcgat gatcgtcgct cagttcgagc ggcgcatgcg
    27301 ggggctcctg agctccgcgc agcgggcgag ggcggtatcc ctgggaacgg acgcggttcg
    27361 cgctgcgttg caagcaaata ggggcgatct attgattcgc gcaaaggatt cgcgtggtcg
    27421 cgcggatgag ctggcgcgtt ccgcgaccgc gattgcttgc gcgacggcca cttgggggac
    27481 aaaggcgagc ttgggggacg cgtttgatcg ttcggagctc ggcgtcctct tggtgacgga
    27541 tcgcggcatc gctcgcgcaa tcctagattg cctgacgcgg gtcgaggccc taacggagga
    27601 tggatgagta aggttcgagt ctacgaggtc gcgcgagagc tgggggtcga gaaccgcgac
    27661 ctgatccagc gaattgcgac tcttgggatc caggtgcgca accacatgag cgtcctcgat
    27721 ccgatcgaag tggaccgcat caaacgttcg ctcggcaaag accgcagcga ggcgatggtc
    27781 gaagagcgta ttcgtccgac cgtcgtccgt cgtaagcggc gtaagcctgc ggaaacggcc
    27841 gagcctgcgc cggcagagac gacagcgaca ccggtccccg tcgatttgcc gagagctgag
    27901 ccggtggcgg ccagccccag tcccgaaccc gttttcgagc cggagcccca gcccgtcgaa
    27961 gcacaagctc cggcgcaagc gccggccgca gcgccggaaa gcgactcgcc acgggagccg
    28021 atggcggcgg aaccgggacc cgcgcctgca cccgcgcttc caccggaaga acaaggtcgt
    28081 ttctctcacg atgcgcttcc tcctggcgtc atgcgccgcg gcaaggccaa agccccaagc
    28141 gcgacccctc tttcggaggg tgcgcggcgg cgcatcgtgg ccgagcacgc cgcacaacga
    28201 gagcagcacg cgcctcggcg tcgcgagatc cggggccgct cttcaatcgg tcctaccggc
    28261 aggccgcagg ggcgccctgg caagaagagg ctgcagcctg ggaaaaagcc aaaacagacc
    28321 gaaattacgg ttccgagcgc acagaagcgg gtcatccgca tcgaagacaa cgttcaactc
    28381 caaaccttgg cgcagcgaat gagcctcaag gcgacggacg tcctcatgaa gctgatggag
    28441 ctcggtgttt cgggcgtgca catcaacagc actctcgatg cggacaccgc ttccgtcttg
    28501 gcgaacgagt tcaactacga ggtcgagaac gtcgcccgga gcgaggacga aatcgtcggt
    28561 gatgcccgag gcgagttcga agacaaagcc ggagatcgtc agcatcggcc gccggtggtc
    28621 acggtcatgg gtcacgtcga tcacggcaag acttcgttgc tcgacaagat ccgcgcgagc
    28681 gatgtggttg caggagaagc gggcggcatc acccagcaca tcggcgccta ccgggtcgat
    28741 acggatcgcg gcaccgtggt tttcctcgat acgcccggtc acgaggcgtt caccgccatg
    28801 cgtgcgcggg gcgctcaggc caccgacatc gtcatcctgg tcgtggcggc cgatgatgga
    28861 gtcatgcctc agaccagaga ggccgtgaat cacgcccagg ccgcgaatgt tccgatcgtc
    28921 gtcgcagtca acaagatgga caagccggag gccaatccgg acaacgtgaa gaacgagctg
    28981 gcggccctcg ggctgcagcc cgaagagtgg ggcggtgaga ccattttcat cccgtgctcg
    29041 gccatgacgg gcgagggtgt cgactccctg ttggagaatg tcgtgcttca ggccgagctc
    29101 ctcgagctca cagccaactc gaacattcct gcggagggcg tggtcttgga ggcatatctc
    29161 gatcgcggcc gaggtccagt ggctaacgta ttgattcgag acggttcttt ggacaccggc
    29221 gactacgtag ttgctggcgg tgcatggggg cgcgttcgag caatgaccga cgaccgcggc
    29281 aggcagttga cctccgcggg ccctgccacg ccggtagagg tgctcggctt ggcggagcta
    29341 cctgcggccg gcgacctatt ctacgaggtc acagaccagc gaaaggccca ggaggtcgct
    29401 ggcgctcgca agaagcccgg caccagcagc atggcgcctg caggcggcat gcggggcctc
    29461 gatcagttcc aggctctcat gcagtcgggc gacgtgcagg agcttcgtct cgtcgtgaag
    29521 gcggacgttc agggttccgt tgaggcgctg caaggtgcgc tcaccgacct tgcgaccgag
    29581 aaggtcaagg tcaacgtcat ccacaccggg gttggcggca tcaccgagaa cgatgtgatg
    29641 ctcgcgagtg catcgcaggc gatcgtactc ggcttcaacg tgcgcccaca gggtaaggcc
    29701 tcctcgaccg ccaagaagga aggcatcgag atccgcacgt acagcgtgat ttacgaagcg
    29761 atcgacgaag tgaaagcggc gatgaagggc cttctcgccc caaccatcgt gcaggcagac
    29821 ctcggcaagg cggaggtcag gcagaccttt ggcattccca agcttggcac gatcgcgggc
    29881 tgcatggtta ccgaaggcaa aatcaaccgt ggtgcgcggg cacggctcgt gcgtgacggg
    29941 gtcgtggtgt gggaaggtcc aatgggctcc ctccgtcgct tcaaagaaga cacgaaggaa
    30001 gtccaggccg gcatggaatg cggcatcggc cttcagggct tcaacgacat caaagagcac
    30061 gacatcatcg agtgctacga ggaaaagcag gtcgaggcga cactcgagtg agctgatcgc
    30121 cgagaggtcc gaacatggtc gttggcgtgt gccagatttc gctttccttg ccgggtgtga
    30181 gctcgctcaa agcgaagcgc tcaatcgtgc gcaaggtgct ggatcgaact gcaaatcgtt
    30241 tcaacgtcgc ggtcgccgaa gtcggacagc tggacgtaca cgggcaatcg acgctcgggt
    30301 tcgccgtggt ctcgaatgat cggcgtcatg cgaattccat gctggattcc atcgcggagt
    30361 tcgtggaagg ctccgcggac gcggtggtct tgaaccggtc gacagagctg ctttccttgg
    30421 gtgagctcac ggattggacg ggctgaacga tggtacaaaa aagctttcga agagcagatc
    30481 gcgtttctga gcggattcga agcgagctca tggagctctt gattcgtgga gccatgcgtg
    30541 acccagccgc caaggacgtc gtgatatcgg cggttcgaat gaccgatgac ttgagcatcg
    30601 cccgcgtgta tgtgcgagtg ctcggggaga ttggcgcgga gcagcaggac gcggtagtcg
    30661 acgcactcaa gcgcgggagt ggttttcttc ggcgggaaat cggacagcgg cttcaacttc
    30721 ggcatgtgcc gtccttggag ttctactggg atgaggtcgt cgattctgcg attcgcatcg
    30781 agtcgattct cgatgagatt cgcgagacgc cgagctcggg gccggagggg tcaaagtgat
    30841 cgaggaagtc cttgagattg cccgcgatgg ggaacgcttt ctcgtggctt gccaccggcg
    30901 ccccgatgcg gattcgctgg gatccgcgct cggactgatc aagtgccttc gtgccgtcgg
    30961 caaggaggcg accttgttca tgcctgaggc cgttcccgac tcgctgctct tcctcatgga
    31021 cgatgagaag ggtattgcgg cgccgccgca gggggcgcgc ttcgacgcga cgttcgtgat
    31081 ggacatcgcg gcgaaagcgc tcttgccacc gggatttccg aagcccgagg tcaccggccc
    31141 cgtcgtcatc atcgatcatc atcacgctca cgatgacgta ggcgacatcg tcttcaggga
    31201 caccgatgcg gccgccaccg gagaggtcgt cgtccagctg atggaggcct tggggctcga
    31261 atccctccca gaaggggcgg cgaccccctt gtatgctgcc atcgtgtcgg ataccggtgg
    31321 ctttcgctac gcgacgactc ggcccgcgac cctacgactc ggggctcgct tggtggaagc
    31381 cggggcaaac ccctggcagg tggcctacca gttgttcgag cgctgggagc ccgcccggct
    31441 tcggctgctt tcagcgatca tcgcgacgct cgaggtccag ttcgacgggc gcgtggcgct
    31501 gatgcgagtg acccggcaga tgctcgagat atgtggagcc aacgacgaca tggtcgaggg
    31561 catggtcaac tacgcgcggc gcgtcgaggg cgtcgaaatc ggggccttgc tctgggagtg
    31621 gccggtgcgc gatgcggatg gcgaacacct ggaaacgaag atcagtctcc gttcgagcgg
    31681 aacggccgac gtatcggtca tcgcggcggc gcttcgcgga ggtgggcacc gcgcggcggc
    31741 tgcgacgcag ctcaatatca cgatggacga ggctgagaga aggctgcacg cggagctgac
    31801 tgagtacttg ggatgaacgg catcctgatc gtcgacaagc cggcgggatg cacttcacac
    31861 gatgtcgtcc agcgggtccg caaggctttg cggcagcgat cggttggcca cgccggcacc
    31921 ctcgatccgc tcgccacggg tgtgctggta gtggcgatcg gagaggcaac gaagctcgtc
    31981 tcgcatcttc aggcggacga caaacgttac gaggtgacga tagcgcttgg cgtggaaacg
    32041 gactcgctcg acgccgacgg cgagatcacc cgtaccctag cggtgccgcc gctcgatcgg
    32101 gccaaggtcg agcaggcctt gcgtggattc atcggccggc acccccaaga ggcgccaaag
    32161 ctcagcgcga tcaagcttgg cggcacgcct cttcaccgcc gaattcggcg aggtgaagat
    32221 gtctcggccc cggtccggga agtggagctg tacgaagcga aggtcgagga ggtcagcggg
    32281 gatgcgattc gcctgagtct ccattgtgcc aaggggttct acgttcgctc gctggcccga
    32341 gacctggccc tcgagctagg gaccctcgga catgtgagag cccttcgtcg cacggccagc
    32401 ggtggttttt cgatcgagca ggcccttcat ggggatggca tgacggctga cgccatcgcg
    32461 gagcgcctga ttccgcttcg agaggcctgc cgatcactca ggcccgtcga gctcaccgac
    32521 gaaggggttg ctcacgcgcg acacgggcgc ccaatcgctc caaacctggt gcgcggcgac
    32581 gcatggcagc aagcctctgc cgaggaatcc ttggcgctct tcgctccgga cggggagccg
    32641 gtggcactcg gtcggcgcaa cgacgagggt attcgcgtgg tgcgcggctt cacggtgccg
    32701 tctcccgatg tggtaagccc ttagcgtgga gcggacgcgt tacctagtgg tgggcgccgg
    32761 cgtgaccgga ctcgcgtttg cagattggct gcgcgatgag gactgtctca ttctcgaagc
    32821 gaacgaggag attggcggct attgccgcac catcaagcgc gacggcttca tctgggatta
    32881 ctcgggccac ttttttcact ttcgccatcc ggaaatcgag cgatacctcg tcgaacgcat
    32941 gggcgcgcaa gcggttcgtc gtatctcgcg ggaaagccga atttggtatg gcgaccggtt
    33001 catcgacttc ccttttcaac gcaacatccg tcagctctcc gaggccgact accaagactg
    33061 ccttcgcggg ctccgtgaaa gaaccaagga agagggcggc aatttcaagg acatgctcct
    33121 tgcacgctac ggccggggca tagccgagaa gtttctcatt ccgtacaacg agaagctcta
    33181 cgcgttgaac ctctccgcgc tcgatgccga tgccatgggg cgcttctttc cccatgtcag
    33241 cgcagagcag atcctggacg aggcgcttgc gccttcggcc gatggataca acgcgacatt
    33301 tacatatccc gagggcggcg cgattcaata cgtgcaggcg ctcgctcgcg ggattcgacc
    33361 cgaacaaatc tcactgaacg agcgtcttct gagtctcgac cttgaacaga aagtcgccac
    33421 gacgacgaga cgccaaatac gctacgactc gctgatcagt agcgcgccgt tggtccggct
    33481 gctcgagatc tgcggcctcg agtacgacgc gtcgattctg agccacaaca aggtgcaggt
    33541 cttcaacctg ggtttcgata gcaaggggcc cgcggacgtg cactggatct actat
//
LOCUS       DASRZF010000193         7092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1854997, whole genome shotgun sequence.
ACCESSION   DASRZF010000193 DASRZF010000000
VERSION     DASRZF010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 7092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7092
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1854997"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /locus_tag="VFG22_08615"
     CDS             complement(<1..1085)
                     /locus_tag="VFG22_08615"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HET6416347.1"
                     /translation="MVRIWLVGFGFVCVAATSACSESSSSSGGNVEIVASEDGSVEVL
                     VGGKPTFALAPTGPVARTFSERAVGVGTIGFERSSEITDPLTVRSVSNEGTGARIEYA
                     NASGSRTATLTANVIDDQLGEFRLELSGPAADSIAVSIRCDADGTFHGFGEQYNATNQ
                     RGEAFELLVNEQGNGRDGSPGVSVGDEHTTYFPMPYYVDARGFGALFVTERRVDVDLC
                     ASDQDVAWIEVISGAPVQWRVFLGPSGLDVIRQLGDFVGRPTQPPVWAYNRWMAGQGG
                     RDNVLAEVAALEAADVPVAAFWVQDWGGIRRNFDGGSGVQYVWSADEDLYPSFASMVS
                     DFHEQGYKFLAYVNPFIVDPSSVQEVD"
     gene            complement(1161..2240)
                     /locus_tag="VFG22_08620"
     CDS             complement(1161..2240)
                     /locus_tag="VFG22_08620"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HET6416348.1"
                     /translation="MTKKIAAWLGSVLLALFLYLFLWPVPIDPVAWSPDPAPALDGDF
                     SENRVLQGMELFATPNSHGPEDVAIDSEGRAYVGVEEGPILRYAADGSDPQVFTDTGG
                     RPLGLDFDTNGNLIVADAVKGLLSIDPNGEITVLCTEAGDRPFRFTDDVDVDSQNVAW
                     FSDASDKWGQENHAMEEALESAANGRLIKYDIASGECTVVLDELYFANGVAVSPDETY
                     VLVNETMRYRVKRVYVRGPRAGEVESFIDNLPGFPDGISTGEDGIFWLAIFAPRNELL
                     DLAGPKPWLRELIYRIPAALQPKPKRHPFVLGLDADGKVVRNLQDSDGVRFAKSTSAE
                     QMGDWLYIGSLTEPSWGRIRISSLP"
     gene            complement(2243..3031)
                     /locus_tag="VFG22_08625"
     CDS             complement(2243..3031)
                     /locus_tag="VFG22_08625"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET6416349.1"
                     /translation="MRSPALAAEHDKQGWLALYSDDAVVEDPVGTPASHKGRRRGRLG
                     DELGRFYEAFIAPSGIRMSTRQDVVSDRYVFRAVDIHTTNLSTGLEMKVPANLLYEII
                     PADRGLAIRRMQAHWELSRMSRMLMSKGSLGFRTIAVMNWSMLRAFGPKWLMAYFKAS
                     QKGVGRSGKELVGRLASEVGHHGSSAFSSDAIVELPGGIKVSAPAFLEGCSSLEVRDL
                     LASGETVSGLASLDWGGRKREAALVAELDEAGTRVHRFRLFWEG"
     gene            complement(3152..3811)
                     /locus_tag="VFG22_08630"
     CDS             complement(3152..3811)
                     /locus_tag="VFG22_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416350.1"
                     /translation="MSKDSCAGTAATSTLRSLTALAVCVAVLGIAERVAAQRTLMSSP
                     TCEDLTIMDFLARLDEEQRKHKRGLDAEDRRELSQLRREVCAPYVGYGVTRHPTATWS
                     NGRTIVRNYLELNHPNGRIAKSRAGEWFYPSGARAKSALGQWEYPNGFLARTARGENW
                     YTREGKYVENLEKVRADACEYLGKELCPLGKADEDLLAVTVMGLINRTAPPREEQKPK
                     N"
     gene            complement(3804..5999)
                     /gene="ppk1"
                     /locus_tag="VFG22_08635"
     CDS             complement(3804..5999)
                     /gene="ppk1"
                     /locus_tag="VFG22_08635"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HET6416351.1"
                     /translation="MDSTSTSNGHPSSNVAPDASARAGEEIDLDASEYYLNRELTWLN
                     FVKRVLHEAEDDRTPLLERVKFLAITASILNEFFMKRIGGLKQQVAAGVHKLTIDGRT
                     PMQQIREAHEIIRALTVEQQKVWVGLRKKLEAIGVHVTPHESLPDDDKRWLRDHYLDN
                     IFPLVTPQAMDPAHPFPFVSNLSLNLLVSLHYPGDPTPLLARVKVPLGSGVPRFIQIR
                     DSLVFVALEEIVASNLDLLFPGMEIERTELFRVTRNADVESDEDKADDLLALIESELQ
                     ERKFAPIVRLEVVQGMDSAHRGRLAAELGLDEQADVFEVDGFLGLADLFQLAVLDIPT
                     QRYPDHRPIDAPVLVGRSNVFHAIRDNGAILLHHPYEAFGTSVERFLREASEDPKVRA
                     IKMTLYRTSSKSRVIQHLINAALNGKQVAVAVELKARFDEEANINWAGKLEQAGIHVT
                     YGVVGLKTHCKVILVVRRDHDGLRRYVHIGTGNYHEETAKLYSDVGLLTCDPSIGSDA
                     TELFNYLTTGYKPSRAYKKLLPAPKILKPALLKKIRREIRRVSEGKKGLIQIKTNALE
                     DVDITRALYEASQAGVHVDLIVRDSCRLRPHVPGLSENVSVVSVVGRFLEHSRIYYFQ
                     NGGEEEYYIGSADCMTRNLVSRVEVLAPVEPPALQAELRTILDALLSDQRSAWDMMPD
                     GSYVQRNGGKDVEGTHQQMIKWAEGRFAEANRLRRRSARGPRGLATSDE"
     gene            6138..6776
                     /locus_tag="VFG22_08640"
     CDS             6138..6776
                     /locus_tag="VFG22_08640"
                     /inference="COORDINATES: protein motif:HMM:NF037005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6416352.1"
                     /translation="MTDQLTPRRKPSQERSRDRVERILDATAALLIDTAADKITTAAI
                     AETAGVPIGSVYQYFPNKLAILAELARRVMEEVDAKTASLIAADFGVLPWDQAIDRAI
                     DATMEGYAEQPGYLQLLLSIRPTPEFRAITDESNQRVAAMLAFHPALQSLIPPDRIEL
                     VTRAAIQAANSLQDWALSEDDALRRNRIISEMKTLLKGYLAVYMDESSPFRS"
     gene            6891..>7092
                     /locus_tag="VFG22_08645"
     CDS             6891..>7092
                     /locus_tag="VFG22_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416353.1"
                     /translation="MNFKVLISVIFAAAIAVVGCDSSSGTGGTGGTAGTGGSAGTGGS
                     AGTGGTGGTGGTGGGTPVTVDCD"
BASE COUNT         1391 a         2226 c         2102 g         1373 t
ORIGIN      
        1 tcgtcgacct cttgtacgct gctcggatcg acgatgaatg ggttgacgta cgcgagaaac
       61 ttgtagccct gctcgtggaa gtcggaaacc atcgaagcga agctcggata gaggtcttcg
      121 tcggcactcc atacatactg aactccggat ccaccgtcga aattcctgcg gattccgccc
      181 cagtcctgaa cccagaatgc ggcgacagga acgtccgccg cctcgagggc tgcgacttcg
      241 gcaaggacgt tgtcgcgtcc accttggcct gccatccacc ggttgtaggc ccagaccggg
      301 ggttgggtcg gacgcccgac gaaatctccg agctgacgga tcacatcgag cccagacggg
      361 ccgaggaaga cacgccattg aactggcgct ccactgatga cttcgatcca ggctacgtct
      421 tggtcgctgg cgcagaggtc gacatcgacg cgacgttccg tgacgaacag tgccccgaaa
      481 cctcgcgcgt cgacgtagta cggcatcggg aagtacgtgg tgtgctcatc gccaaccgaa
      541 acaccagggc tcccgtcgcg cccgttaccc tgttcgttga cgagaagctc gaatgcctca
      601 ccacgctggt tcgtcgcatt gtactgctcg ccaaacccat gaaacgttcc atccgcgtcg
      661 cagcgaatgc tgaccgcgat cgagtcggcg gccggcccag aaagctcgag tcgaaactcg
      721 ccgagttggt catcgatcac gttcgcggtc aatgtcgcag tgcgagagcc gctggcattc
      781 gcgtactcga ttcgtgcacc cgtgccctcg ttcgacacgg atcgcactgt gagcggatcg
      841 gttatctccg agctgcgctc gaaccctatc gtaccaacgc ccaccgcgcg ctcgctgaac
      901 gtccgtgcga ccggccccgt gggcgccagc gcaaacgtcg gcttgccgcc aacgaggacc
      961 tcgacggagc cgtcttcact ggcgacgatt tcaacgttgc cccccgaaga agagctgctc
     1021 tcgctgcaag cgctcgttgc tgcgacgcaa acgaacccaa agcctacaag ccaaattctc
     1081 accatcgcgc cgctcccatc taagtaatgg acgcatagtt caaggttcga cacccatgag
     1141 caagcgcacg gactgaggtc tcagggaagc gaggagatcc gaattcgacc ccagctcggc
     1201 tccgtgaggc tcccgatgta cagccagtcg cccatttgct cggcgctcgt ggacttcgca
     1261 aaacggacgc cgtccgaatc ttgcaaattg cgcacgacct ttccgtccgc atcgagcccc
     1321 agaacgaaag gatggcgctt cggtttgggc tgaagcgcag ctggaattcg gtagatcagc
     1381 tcgcgcaacc aaggcttcgg tccagccaag tccagaagct cattgcgagg cgcgaatatc
     1441 gccagccaga aaatcccgtc ctcgcccgtg gaaatcccgt ctggaaaccc gggaaggtta
     1501 tcgatgaagc tctcgacctc ccccgctcga ggcccgcgaa cgtagactcg cttgacccga
     1561 taccgcatgg tctcgttcac caagacgtag gtctcgtcgg gtgaaaccgc gacgccattc
     1621 gcaaagtaaa gctcgtcgag gacgactgtg cactcgccgc tcgcgatgtc gtacttgatg
     1681 agtcggccat tcgcggcact ttcgagagcc tcctccatcg catggttttc ctgtccccac
     1741 ttgtcggagg cgtccgaaaa ccaggcaacg ttctgcgaat cgacgtcgac gtcatcggtg
     1801 aatcgaaagg gccgatcgcc cgcttcggtg cacaagaccg tgatctctcc gttcgggtcg
     1861 atcgagagca agcccttgac cgcgtccgcc acgatcaagt tgccattcgt gtcgaagtcc
     1921 aaaccgagcg gccgtcctcc ggtatccgtg aagacctggg ggtccgagcc atcggcggcg
     1981 tagcggagga ttgggccctc ctcgactccg acatacgccc tgccctccga atcgatcgcg
     2041 acgtcctcgg gcccgtggct gttcggcgtc gcgaatagct ccatgccttg caggacccgg
     2101 ttctcgctga agtctccatc gagcgccggc gcgggatcgg gggaccaagc gaccgggtcg
     2161 atcggcacgg gccataggaa gagatagagg aagagggcga gcagcacgct gcccagccag
     2221 gccgcaatct tcttggtcat cgtcatccct cccagaacaa acgaaagcga tgcactcgag
     2281 tgcccgcctc atcgagctca gcaaccaagg cggcctctcg cttgcggcct ccccaatcga
     2341 gggacgccag ccccgacacc gtctcgccgg acgcgagaag atcccggacc tcgagtgagg
     2401 agcagccttc gagaaatgcg ggcgcggaca ccttgatgcc tccgggaagc tcgacgatcg
     2461 cgtcagacga aaacgccgag ctcccatggt ggccgacttc gcttgcgagc cgtccgacca
     2521 gctctttccc ggatcgcccg acacctttct gcgatgcctt gaagtatgcc atcaaccact
     2581 tcggtccgaa cgcacgcaac atgctccagt tcatcaccgc gatggtcctg aatcccagtg
     2641 agcctttgct catcagcatt cggctcattc ggctcagctc ccagtgtgcc tgcatgcggc
     2701 gaatcgcgag ccctcggtcg gcggggatga tttcgtacag aagattcgcg ggcaccttca
     2761 tttcgagccc ggtactgagg ttggtcgtgt ggatgtcgac ggcgcggaag acatagcgat
     2821 cggagacgac gtcctgacga gtcgacatcc gaattccgct cggtgcgatg aacgcttcgt
     2881 agaagcgtcc aagctcgtcc cccaatcggc cacgacgtct gcctttgtga gacgccggcg
     2941 tgcccacggg gtcttcgacc acggcgtcat cagagtagag cgcgagccat ccttgcttgt
     3001 cgtgctctgc ggccaacgcg ggcgacctca tggcggcccg aaggagggtg tcccgctcac
     3061 tcggcaagga cgctgactgg ggcgaaggcg cattcatcag agcgcctttt accacatcga
     3121 cgccctactc gtcttgggcg gattcgctgg ctcagttttt gggcttctgc tcctcacgcg
     3181 gtggcgcagt ccggttgatc aggcccatga ccgtcactgc gagaaggtct tcgtcggcct
     3241 tgccgagagg gcaaagctcc ttgccgaggt actcacaggc gtctgcccgc accttctcga
     3301 gattttcgac atacttccct tcgcgcgtgt accagttctc accccgcgcc gtacgggcga
     3361 ggaagccgtt cggatactcc cactgaccga gcgcactctt cgctctcgcg ccactcggat
     3421 agaaccactc gcccgctcgg ctcttggcga tgcgcccatt gggatgattc agctccaggt
     3481 agtttcgaac gatggtccga ccgttggacc aggtcgcagt gggatgccgc gtgaccccat
     3541 agccgacgta cggggcgcag acttcgcgtc gaagttgcga cagctcacgc ctgtcttccg
     3601 cgtccaagcc gcgtttgtgc ttcctctgtt cctcgtcgag acgagcgaga aagtccatga
     3661 tcgtgagatc ttcgcaggtc ggggatgaca tcaatgtgcg ttgtgctgcc acgcgctcgg
     3721 ctatcccgag aaccgcgacg cagaccgcaa gcgcggtcag ggatcgcagg gtcgaagttg
     3781 ccgccgtacc tgcgcacgaa tccttactca tcggacgtgg cgagtcctcg cgggccccga
     3841 gcactgcggc gccgcaaacg gttggcttcc gcgaaacggc cttcggccca tttgatcatc
     3901 tgctggtgag tgccctcgac gtccttgccg ccatttcgtt gcacgtagct tccgtccggc
     3961 atcatgtccc acgcgctgcg ctgatcactc aagagcgcgt cgagaatcgt gcgcagctcc
     4021 gcctgcaagg cgggaggctc gaccggtgca agcacctcga cccgactgac gaggtttcgc
     4081 gtcatgcagt ccgccgatcc gatgtagtac tcttcctcgc cgccgttctg gaagtagtag
     4141 atacgcgaat gctccagaaa ccggcccacg acgctcacga cggaaacgtt ctcggaaagg
     4201 ccaggcacgt gcggacgcaa tcggcacgaa tctcgaacga tcaagtccac gtgcacgccg
     4261 gcctgggagg cttcgtacaa agcacgggtg atgtcgacgt cctcgagcgc attcgtcttg
     4321 atctggatga gtcccttctt gccctcgctg actcggcgaa tctcgcgccg gattttcttg
     4381 agaagcgcag gcttgaggat cttcggagcg gggagaagtt tcttataggc gcgactgggc
     4441 ttgtagcccg tcgtcaggta gttgaagagc tcggtagcgt cgctgccgat gctcggatca
     4501 caggtgagca agcccacgtc cgaatagagc ttggccgtct cctcatgata gtttccggtc
     4561 ccgatgtgaa cataccgacg caatccatcg tggtcgcgac ggaccaccag gatgaccttg
     4621 caatgggtct tcaacccaac gaccccgtag gtaacgtgaa tcccggcttg ctcgagcttg
     4681 cccgcccagt tgatgttggc ctcctcgtcg aagcgagcct tgagctcgac tgcgaccgcg
     4741 acctgcttcc cgttcagcgc ggcgttgatg agatgctgaa tgacgcgtga tttagaggaa
     4801 gttcgataga gcgtcatctt gatggcgcgc accttcgggt cttcgctggc ctcccggaga
     4861 aatcgttcga cggaggtgcc aaaggcctca tacgggtggt gaagcaggat ggcgccattg
     4921 tcccgaatcg cgtggaaaac gttgctccga cccaccagga caggagcgtc gatcggtcga
     4981 tgatccgggt accgctgggt cggaatgtcg agcaccgcca attggaagag gtccgccagt
     5041 cccaagaacc catcgacctc gaacacgtca gcctgttcgt cgagaccaag ctccgcggcg
     5101 agacggccac ggtgcgcgct gtccatgccc tgcacgacct cgaggcgaac gattggggcg
     5161 aatttccgct cttggagctc tgactcgatc agggcaagca ggtcatctgc cttgtcctca
     5221 tcgctttcga cgtcggcgtt gcgcgtgacg cggaaaagct cggttcgctc gatctccatc
     5281 cctgggaaca gaaggtcgag attgctggcg acgatttctt cgagggctac gaacacgaga
     5341 gaatcgcgga tctgtatgaa gcgcgggacg cccgagccca aaggtacctt gacccgcgcc
     5401 aagagtggcg tcgggtcgcc ggggtaatgg agcgaaacca ggagattcag agaaagattg
     5461 gagacgaatg gaaacggatg ggccggatcc atcgcttggg gcgtcacgag tgggaagatg
     5521 ttgtccaggt agtggtcgcg aagccaacgc ttgtcgtcgt ccgggagcga ttcatgcggg
     5581 gtcacgtgca cgcctatcgc ctcgagcttc ttgcgaagcc cgacccaaac cttctgttgc
     5641 tccaccgtga gggcgcgaat gatctcatgc gcctcccgaa tctgttgcat cggggtgcgg
     5701 ccatcgatcg tgagcttgtg gaccccggcc gccacctgct gcttgaggcc gccgatacgc
     5761 ttcatgaaga actcgtttag gatcgatgcg gtgatcgcga gaaacttcac gcgctcgagc
     5821 agtggcgttc gatcgtcctc cgcctcatgg agcacgcgct tcacgaagtt gagccaggtc
     5881 agctcccgat tgagatagta ctcggaggcg tccagatcga tctcttcgcc cgctcgcgcc
     5941 gatgcatcgg gtgcgacatt tgacgacgga tgcccgttgc tggtcgatgt ggagtccatg
     6001 gccgagaaat cgtgaaatcc cagtgtagcg cgcaattgtc gttcggatct aggggccgcc
     6061 tctcggaagg gctagtcatc cactgcattt tccgctaatt ccgggcgccg acgaggtctt
     6121 cagacaatcc tgaccccatg accgaccaac tcactccgcg acgaaagccg agccaggagc
     6181 gttctcgtga tcgcgtggag cgaattctcg acgcgaccgc ggcgctcctg atcgacacgg
     6241 cggccgacaa gatcaccacc gcagccattg cggaaaccgc tggcgtgccg attgggtcgg
     6301 tgtaccagta cttcccgaac aagctcgcga tccttgcgga gctcgcgcgc cgggtcatgg
     6361 aagaggtcga cgcgaagacc gcaagtctga tcgccgcaga ttttggcgtg cttccctggg
     6421 atcaggcgat cgaccgcgcg atcgacgcga cgatggaagg ctacgcggag caacccgggt
     6481 acttacagct tctgctgagc attcgtccca ctccggagtt tcgcgcaatc acggacgagt
     6541 ccaatcaacg cgtcgccgca atgctcgcgt tccatccggc cctccaatct ctgatcccgc
     6601 cggatcgcat cgagctcgtc actcgcgctg ccattcaagc ggcgaattct ttgcaggact
     6661 gggcgctctc cgaagacgat gcgctccgca ggaatcgaat catcagcgaa atgaaaacgc
     6721 tcctcaaggg ctacctcgcg gtttacatgg atgagtcgag cccatttcgg agctaaaaac
     6781 ccattgattt cagttatttg agaaaataag caaattgtga gtgatactca cttttcaagt
     6841 tcggcaattg gtgatacaaa cggaaacctt ttggaggagg agacccctaa atgaatttca
     6901 aagtacttat cagcgtgatt tttgcggctg ctatcgcagt cgtcggctgc gatagcagca
     6961 gcggcactgg cggcactggc gggacggcag gcaccggcgg cagcgcaggt accggcggca
     7021 gcgcgggcac tggcggcacc ggcggcaccg gcggcaccgg cggcggaacc ccagtcaccg
     7081 tggactgcga ca
//
LOCUS       DASRZF010000194         7137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3570781, whole genome shotgun sequence.
ACCESSION   DASRZF010000194 DASRZF010000000
VERSION     DASRZF010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 7137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7137
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3570781"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2511)
                     /locus_tag="VFG22_08650"
     CDS             complement(<1..2511)
                     /locus_tag="VFG22_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416354.1"
                     /translation="MRWLMRIVSWFLGWPMLALVSVLASVLFHLDTDLGRKIGRDMLN
                     DFVSEQMAGTLHAGYITQLRLWHTIVKDTFVYDPDGRAIIYGETVELGIDPIAGLRGR
                     LRFYYADLTNGWVDLVDDGEGAPTFLAAFEEADPTPLVGEPFHAIVDNIEIRNLEVTG
                     ELLGLEGLRVVDMDTRGRMEFYWITEIEIRSADGRIVSPFPFEGSLDSLVGSVRTDAR
                     GTEVTAEVSRSHQERATAELVYRPREGSAPEDPFDLDLFVRVEPISAETLHDVGFDWA
                     ESLKGEATGWVRLWGPAGDYRLRADLDTAGGRARLNGELPSEGATRIRISSPRAKLAK
                     VIGGAPDVEVGGKIEIVTDPANEDVLGVAIDAEAFEYGEVHVPAFVAKVRTRADGIDI
                     DSVQSEYAGGNLTLSGHVEYKGVSEIHARGYVPEVSADPNFQKFVPDLEGGAEFDLHI
                     RQAVGGDFETKGSVRFDHLDYGALSAHFLMLEGRVWGDPAKPMLDLELNGAAVRVAGY
                     PLGSGAATIKGGPQQYTAAGAFNAQGDRHASFDARVAVTGNVYRLDVDTVELSVGDLS
                     WRGSVKNAALDLDEGISFDRILMGKGSQRLEAQGVWLFDGPDDIRADLQDFDLAVLRI
                     LYPSQAPNVEGRVDLHFEFRGDLEKDPTIVAEGTLTDATLWEVAPVDAAYLIHYEKGV
                     LDADAQVNFGGRGNFTLSTTGFVTSVPQGIVQSLREGVYETTLSTGAMDLTLLELVLE
                     EDMPNVHGYADASIRFSGPIDASSFEGDIHIPSLIIEDWGPVDLTSDFRYEYGALLAQ
                     VRLADDKGELIESEGSLLVDLVHLARAPGEAVEALATSP"
     gene            complement(2511..4655)
                     /locus_tag="VFG22_08655"
     CDS             complement(2511..4655)
                     /locus_tag="VFG22_08655"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HET6416355.1"
                     /translation="MARRRCICLLLVLASGCKTIPEDAYGIDRIRFTGMEKLDPEALQ
                     ACLASQERDRVTVSFGTNADPKCGVPPFDGKRVSFNLWSKLRKGWPTLDSAVFERDMQ
                     RVERWYESRGYYDAAVVSTAFDPASAASDDRIVVAPGGTAPCEREDEGEGCKLSIEIE
                     VEEGEPILVDSIQVTGHEGWDSGKRDRIEKSIELEIGKPFDEAIYERSKRAIRRQLKE
                     ASYACGTVSGRVEIDRESKRAFVTLLIEGGPSCVLGDITVTGNADLPGGTILDVADLK
                     TGQVYSSNKLADAQRAVYALGALASVNVTSTARLDEEERCTGIVDVNIAVEPGRAIRW
                     GIGAGIQSGTITTLTLQQDTRISDFHLFGLFEHRNFIGGFRRLRIEDRPKIVALDPFF
                     RFAQGFIFGNELRVEYRRPAFLEPRTTASIQFRHDWGPDPFELFRRHDFQTRGSLARP
                     FFDGRLLLVGALNYDTFLVVDQDPRFPTSYQVVFLDESVIVDLRDDSRTPRAGFFFQI
                     ASQQSPAKLERSWQYVRVTPEIRGYAPMPLSSTLAFRFGIGAMFIFSRDRDRLDLVGQ
                     ELGPQNYRLRSGGPTSHRGFFGGFLGDPGNLDSLNREGFDARNDGGLRRWEAGVEWRV
                     PVTQAFATALFMDFGDVNRGQEFRFNYIRAAAGFGFRYQTPVGPIRLDFGFLIPKWQI
                     VGEEPQPNAYNIFRFIRGGVPGAIHFTIGEAF"
     gene            4764..6197
                     /gene="miaB"
                     /locus_tag="VFG22_08660"
     CDS             4764..6197
                     /gene="miaB"
                     /locus_tag="VFG22_08660"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HET6416356.1"
                     /translation="MKQYFVQTFGCQMNVHDSRRIEEVLRANGYREASEPALADLLLV
                     NTCSVREKAEHKLMSLLGTFRPFKEARRGTVLAVAGCVAQQEGERLLRKAPFVDIVLG
                     PDNIPELPALIREVEQGAPPITRTVFDMDDPQFLRATPEADRLEVTAYVTVMKGCDER
                     CTFCIVPYTRGSERYRAADEIVGEIADLVSGGVREVTLLGQTVNSWHEPNAEGQPVKT
                     GSKMAESSSQFAELLRRIAREIPELARLRYTSPHPRHITPELVAAHAELDVLPAHVHL
                     PVQSGSDRVLRRMARRYTANEYVARTDALKKAKPGLTLSTDFIVGFPGESEEDFQRTL
                     ELVRRVEFVAAFAFKYSPRPYTPALNLGDEVSEEVKDERLARLFDLIDSQQQAHLNSL
                     VGRELEVLVEGPSPGDTGRFAGRSERNEIVHLIAPAGHDPRGELVTVTVDQPFRHSLS
                     GYMSGAEGRLERGRRTRLSVLDVGGAS"
     gene            6251..6583
                     /locus_tag="VFG22_08665"
     CDS             6251..6583
                     /locus_tag="VFG22_08665"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HET6416357.1"
                     /translation="MALIFVISSFEVRVPGLAHVPLRDKGIHLVEYGVLGWLCAAASA
                     RTWPSASSWRTAAFAVFVTVLWGISDEIHQAFVPGRTPELADVTADLFGSIVGSAARH
                     FLSDRTVS"
     gene            6919..>7137
                     /locus_tag="VFG22_08670"
     CDS             6919..>7137
                     /locus_tag="VFG22_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416358.1"
                     /translation="MNRLKRYFPIPYLLLATLGIGLIIREMIRGPQVSLAEVGALLAI
                     VPLVAFMSSLLMFKRARTSRNQYLMLALS"
BASE COUNT         1309 a         2279 c         2188 g         1361 t
ORIGIN      
        1 gggggaggtc gcgagcgctt ccaccgcctc gccaggcgct cgtgccaagt gaaccaagtc
       61 gaccagcaag ctgccctcgc tttcgatgag ctcgcccttg tcatctgcca agcgaacctg
      121 cgcgagcagc gctccgtact cgtatcgaaa gtccgacgtg aggtccaccg ggccccagtc
      181 ttcgatgatg aggctcggga tgtggatgtc tccctcgaac gaggacgcat cgatcggtcc
      241 ggagaaccga atactggcat ccgcataccc atgcacgttg ggcatgtcct cctcgagaac
      301 cagctcgagc aaggtgaggt ccatcgcgcc ggtgctcagc gtcgtttcgt acacgccttc
      361 gcgcagcgat tgaacgatgc cctggggcac cgacgtgacg aatccggtcg tgctcaacgt
      421 gaaattgccc cggccgccga agttcacctg ggcatcggcg tcgagcacgc ccttttcgta
      481 gtgaatcaaa taggccgcgt cgaccggcgc gacttcccag agggtcgcgt cggttaacgt
      541 gccctccgcc acgatcgttg ggtccttctc gagatccccc cggaactcga agtggaggtc
      601 gactcttcct tcgacgttcg gtgcctgcga cgggtagagg atccgcaata cggccagatc
      661 gaagtcctga aggtccgcgc ggatgtcgtc cggtccgtcg aacagccaga cgccctgagc
      721 ctcgaggcgc tgtgaaccct tgcccatcag aattcgatcg aaggagatcc cttcgtccaa
      781 gtccaaagct gcgttcttca cggagccgcg ccacgacagg tcgccaacgc tgagctcgac
      841 cgtgtccacg tccagtcgat acacgttgcc ggtgactgcc acgcgagcgt caaacgacgc
      901 gtggcgatcg ccctgcgcat tgaacgcgcc tgccgcggtg tattgctgtg gaccgccctt
      961 gatcgtcgcc gctccgctgc caagcggata tcccgccacg cgcacggcag cgccgttcag
     1021 ctcgaggtcg agcatgggtt tcgccgggtc tccccagacg cggccttcga gcatcagaaa
     1081 gtgtgcgctg agcgctccgt agtccaagtg gtcgaagcgg acggacccct tcgtttcaaa
     1141 atcacctccc acggcctgcc gaatgtgaag gtcgaactcg gcaccgccct cgaggtctgg
     1201 cacaaatttc tggaagttgg ggtctgcgct gacttctgga acgtatccgc gcgcatggat
     1261 ctcggagaca cccttgtatt cgacgtgccc actgagcgtg aggttgccac ccgcgtattc
     1321 gctttgcact gagtcgatgt cgatgccgtc ggcgcgcgtt ctcaccttgg cgacgaaagc
     1381 gggaacgtgc acttcgccgt attcgaaagc ctcggcgtcg atggcgacgc ccagcacgtc
     1441 ttcattcgcc ggatcggtga cgatctcgat tttgccgccc acctccacgt cgggcgcacc
     1501 tccgatcact ttggccagct ttgctctagg gcttgaaatc ctgattctgg tggcaccctc
     1561 gctcggcagc tctccattga gccgtgcgcg tccaccggcc gtatcgagat ctgccctcag
     1621 gcgatagtca cccgccgggc cccagaggcg aacccagccg gtcgcctcgc ccttgagtga
     1681 ttccgcccag tcgaagccga cgtcgtggag cgtctcggca ctgatcggtt cgacccgcac
     1741 gaagagatcg agatcgaacg ggtcctctgg cgcgctgcct tctcgtggac ggtagacgag
     1801 ctcggcggtc gcgcgctcct ggtggctgcg gctcacctcg gccgtcacct ccgttccccg
     1861 cgcgtccgtg cggacgctgc cgaccaagct gtcgagactt ccttcgaatg gaaagggact
     1921 cacgatccgg ccgtcggcgg aacggatctc gatctccgtt atccagtaga actccatgcg
     1981 tcctcgcgta tccatatcca cgacgcgaag gccttcgagc cccagcaact cgccggtgac
     2041 ctcgagattg cgaatctcga tgttgtccac gatggcgtgg aagggctcac ccacgagcgg
     2101 cgtcggatcg gcttcttcga acgcggccag gaacgtaggc gcaccttcgc cgtcgtccac
     2161 caaatcgacc cagccgttcg tcaggtccgc gtagtagaag cgaagtcgcc cgcgaagccc
     2221 cgcgattgga tcgataccga gctcgacggt ttcaccgtag atgatcgccc ggccatcagg
     2281 gtcgtacacg aacgtgtctt tgacgatcgt atgccaaagc cggagttgtg tgatgtagcc
     2341 tgcgtgaagt gtgccggcca tttgctcgga cacaaaatcg ttcagcatgt ctctgccgat
     2401 cttgcggccc aggtcggtgt cgaggtgaaa cagaacgctc gcgagcaccg atacgagggc
     2461 cagcatcggc caccccagaa accaactgac gatgcgcatg agccaacgca tcaaaacgct
     2521 tcaccaatcg tgaaatggat cgccccgggc acgcctcctc ggatgaagcg aaagatgttg
     2581 tacgcgttcg gttgtggctc ttcgcccacg atctgccatt ttggaatgag gaagccgaag
     2641 tcgaggcgaa tcgggcccac cggggtttgg tagcgaaagc cgaatccggc cgcagcgcgg
     2701 atgtagttga agcggaactc ttgcccgcga ttcacgtcgc cgaagtccat gaacagggcg
     2761 gtcgcaaagg cttgcgtgac cggaacgcgc cactcgaccc cggcctccca acggcggagc
     2821 ccaccatcat tgcgcgcgtc gaagccctcc cggttgagcg agtcgagatt gcccgggtcg
     2881 ccgaggaacc caccaaagaa ccctcggtgg ctcgtcggac cacccgagcg caaacggtag
     2941 ttctggggac cgagctcctg gccgacgagg tccaggcgat cacggtctcg agagaatatg
     3001 aacatcgcgc cgatgccgaa tcgaaacgca agcgtcgatg agagcggcat cggtgcataa
     3061 ccgcggatct ccggagttac acggacgtac tgccaggagc gctcaagctt ggcgggtgac
     3121 tgctgactgg cgatctgaaa gaagaatccg gcgcgcggcg tcctcgagtc atcgcgaaga
     3181 tcgacgatga cgctttcgtc gaggaatacg acctgatagc tcgtcggaaa ccgtgggtcc
     3241 tgatcgacca cgagaaacgt gtcgtagttg agcgcgccca ccagcaagag gcgaccgtcg
     3301 aagaacggtc gcgcgaggct gccacgagtt tggaaatcgt gccggcgaaa cagctcgaaa
     3361 ggatcgggac cccagtcgtg gcggaactgg atgctcgcgg tcgtccgtgg ctcgaggaac
     3421 gctggccgcc gatactccac gcgtagctcg ttgccgaaga tgaatccctg cgcgaagcga
     3481 aagaacggat cgagggccac gatcttgggg cgatcctcga tacgcaggcg tctgaatccg
     3541 ccgatgaaat tgcggtgctc gaatagcccg aacaagtgga aatcggagat tcgagtgtcc
     3601 tgctggagcg tcaaggtggt gatcgtgccg ctctggatgc cggcgccgat gccccagcga
     3661 atcgcccgcc cgggctccac ggcgatgttg acgtcgacga tgcccgtgca ccgctcctcc
     3721 tcgtcgagtc gcgcagtcga cgtcacgttc accgaagcca gcgcgcccaa cgcgtaaacg
     3781 gctctctgcg catccgcgag cttgttcgag ctgtagacct gcccggtctt gaggtccgcc
     3841 acgtcgagga tcgtcccccc ggggagatca gcattgccgg tcacggtaat gtccccgagt
     3901 acgcaactcg gaccgccttc gatcaagagt gtcacgaacg cacgtttcga ctcacggtcg
     3961 atttccacgc gtccggagac ggtgccgcag gcgtagctcg cttccttgag ctggcgtcga
     4021 atcgcgcgct tgctgcgctc gtaaatcgct tcgtcgaagg gctttccgat ctcgagctcg
     4081 atggatttct cgattcggtc gcgcttgccg gagtcccaac cttcgtggcc ggtcacttgg
     4141 atcgagtcga ccaagatggg ctcgccctct tcgacctcga tctcgatgct cagcttgcag
     4201 ccctcgccct cgtcttctcg ctcgcacgga gccgtgccgc ccggagcgac gacgattcga
     4261 tcatcgctgg ccgcagaggc ggggtcgaag gcggtgctca ccacggccgc gtcgtagtag
     4321 cccctcgact cgtaccagcg ctcgactcgc tgcatgtccc gctcgaacac ggccgaatcc
     4381 agcgtcggcc agcctttgcg gagcttgctc cagaggttga acgaaacccg tttgccgtcg
     4441 aacgggggga caccgcactt gggatcggcg ttcgtgccga aggagacggt gacgcggtcg
     4501 cgctcctggc tcgcgaggca ggcctgcaga gcctccggat ccagcttctc catgccggtg
     4561 aaacggatgc gatcgatgcc atatgcatcc tctgggatcg tcttgcagcc gctcgccaga
     4621 acgagcaaga ggcagatgca tcgccggcga gccatctacc ttaggcttgt agcgcggagc
     4681 gccccagttc cgccagcgaa cgacgtaatt tcggctgttt ggcgagcgcc ccagcgactg
     4741 ctacagctat cgccccaaag cccatgaagc agtacttcgt ccagaccttc ggctgccaga
     4801 tgaacgttca cgattcccgt cgaatcgagg aggtcttgcg agcgaacggt tatcgggagg
     4861 ccagcgagcc cgcgctggcc gatctcttgc tggtcaacac ctgcagcgtg cgcgagaagg
     4921 cggagcacaa gttgatgagc cttctcggca cgttccggcc tttcaaagag gctcgacggg
     4981 gcaccgtgct ggcggttgcg ggctgtgttg cgcagcagga gggcgaacgg ctcctccgca
     5041 aggcgccctt cgtggacatc gttctcggac cggacaacat cccagagctt ccggccttga
     5101 tccgtgaggt cgagcagggc gcgccgccga tcaccaggac cgtcttcgac atggacgacc
     5161 cgcaattcct tcgggcaact cctgaggccg atcgcctgga ggtcaccgcg tacgtcaccg
     5221 tgatgaaagg ctgtgacgag cgctgcacct tttgtatcgt tccttatacc cgtgggtcgg
     5281 aacggtatcg agcggccgac gagatcgtcg gagagatcgc ggacctcgtt tctggcggcg
     5341 tgcgggaggt gaccctgctg gggcagacgg tcaactcatg gcacgagccc aacgccgaag
     5401 ggcaacccgt caagactggg tcgaagatgg cggagtccag ctcgcagttt gccgagctcc
     5461 ttcgacgcat cgcccgcgag attccagagc ttgcccgcct gcgctacacg tcgccgcatc
     5521 cgcgtcacat cacccctgag ctggtggcgg cccacgcgga gctcgacgtc ttgcccgcgc
     5581 atgttcactt gccggtccag tcgggatcgg accgcgtgct ccgacgcatg gcgcgacggt
     5641 acacggccaa tgagtacgtt gcccggaccg atgcgctcaa gaaggcgaag ccgggattga
     5701 cgctttccac ggacttcatc gtcggatttc cgggcgaatc cgaagaagac ttccagcgga
     5761 cgctcgagct ggtgcggcgg gtcgaattcg tggccgcgtt cgcgttcaaa tactcgccac
     5821 gaccgtacac gccggccctc aacctgggcg acgaggtttc cgaggaagtg aaagacgaac
     5881 gcctggctcg cttgttcgac ttgatcgatt cgcagcaaca ggcccatttg aattcactgg
     5941 ttggccggga gctcgaggtg ctagtcgaag ggccgtctcc cggagatacc ggacgctttg
     6001 cggggcggag cgaacgcaac gagatcgtcc atctcattgc gcctgcgggc cacgatccaa
     6061 gaggcgagct cgtcaccgtc acggtcgatc aaccgtttcg ccacagcctt tccggctaca
     6121 tgagtggagc cgaagggcgg ctcgagcgcg gccggcgcac ccgcctttcc gtgctcgatg
     6181 taggaggggc aagctgagcg ccgattcctt ctgggagcgc ctgcgcatct ggcgctttgc
     6241 gctgctgtac atggcactga tcttcgtgat ctcgtcattc gaggttcgag tgccaggcct
     6301 cgcgcacgtc ccgctgcggg acaaagggat ccatttggtc gagtacggcg tcctcggctg
     6361 gctctgtgct gcggcctccg cgcgcacatg gccttcggct tcctcctgga gaacggccgc
     6421 attcgcggtg ttcgtcacgg tgctttgggg gatctccgat gagattcatc aggcgttcgt
     6481 tccgggccgc acgccggagt tagctgacgt gacagcggat ttatttggta gcattgtagg
     6541 ctcggcggca cggcattttc tttcagaccg aactgtaagc tgatgtaccg gaaaactcgg
     6601 ttccgagggc ggggcaccat tgacggttcg agcgccagta tctaaatttt aatccaacag
     6661 gacggtggcg tcctcggaac caaaaataaa aagtttagcc ggcggaaccc cccccaataa
     6721 gtccgccggt tgtccccgcc cggtcgcgtc tccccccctc caagcgaccg ggcgaccctt
     6781 tttaagcccc ggaagcagcg acagtgtcag tgtcagtgac agtgtcagtg acagtgtcag
     6841 cgacagtgac agtgtcagcg acagcgccag tggcagcgtc aacgtaacct tccttcgcct
     6901 cgcccgtcgt agctccgcat gaatcgtctc aagcgctact tcccgattcc atacttgctc
     6961 ctcgccacgc tgggcatcgg gctgatcatc cgggagatga ttcgaggacc gcaggtctcg
     7021 cttgccgagg tcggggcgct gctggcgatt gtgccgctcg tcgccttcat gtcgtctctg
     7081 ctgatgttca agcgagcccg cacgtcccgc aatcagtatc tcatgctggc gctttcg
//
LOCUS       DASRZF010000195         9848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2541834, whole genome shotgun sequence.
ACCESSION   DASRZF010000195 DASRZF010000000
VERSION     DASRZF010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 9848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9848
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2541834"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..589
                     /gene="crcB"
                     /locus_tag="VFG22_08675"
     CDS             209..589
                     /gene="crcB"
                     /locus_tag="VFG22_08675"
                     /inference="COORDINATES: protein motif:HMM:NF014584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HET6416359.1"
                     /translation="MSFVWVAIGGAAGSCLRFWVSLILGPVTAAHGFPWATLAANVLG
                     SLGLGLVFVLGEGRSWFGGDLRLLLGTGVMGGFTTYSAFNLESIGLMQDGAWGRAGLY
                     MGGTMLLCLLAGALGVIVGRAIRG"
     gene            complement(591..2234)
                     /locus_tag="VFG22_08680"
     CDS             complement(591..2234)
                     /locus_tag="VFG22_08680"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HET6416360.1"
                     /translation="MVSLLTTVRDLERLRQIAGVLAVHGFGEVVDRTGLSTLIPGRKR
                     GDGGRMPAGIRLRKVLEDLGPSFVKLGQMMSTRPDLIPEDILVELKKLQDDVPPEPFD
                     VIRQQVERELGASLSEVYQSFEEAPLASASVAQVHRARLRNGEDVAEVVVKVQRPEVQ
                     TVMARDVDLLYWLAKAIVRSMPESHLYRPVSLVEEFDRSVSAELDFSLEADNHERFTE
                     NFRNNPHAKFPKVYREASAKRVLTLEYLEGRKVDAAVCAGASGEIIAKRSVEIIIQQI
                     FEDGFFHADPHPGNIIILGADDDPIIGMIDVGMVGRLAPKMRDRTIDLMLAAVREDYG
                     GIADALFAIGRPQHKINRDAYDAEVATLAQRYLGKRLKDIELGPLLRDLIMGSRKFGV
                     EIPSEFLMLGKSLMTVEGVGKEIYPELDLLEEVRPYFMKLFQQRYSPERMTQDALRGL
                     RRLSVATSEVPMQLEEILEDLRSGAFTVRTQQTQLESAADRLGRRAFSGLVVGSLIIA
                     GAILLSQGSYWLGGLAAGAGALYATLHTARVWLLGKPEP"
     gene            2387..2725
                     /locus_tag="VFG22_08685"
     CDS             2387..2725
                     /locus_tag="VFG22_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HET6416361.1"
                     /translation="MKKVEAIIKPFKLDEVKDALNEVGIKGMTVTEVKGFGRTGGKRE
                     VYRGSAYVVDFVPKVKVEIVVPDSLVVSVIEAIENAAKTGRIGDGKIFVSPIEESVRI
                     RTGERGEAAV"
     gene            2884..3750
                     /gene="asd"
                     /locus_tag="VFG22_08690"
     CDS             2884..3750
                     /gene="asd"
                     /locus_tag="VFG22_08690"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00163.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HET6416362.1"
                     /translation="MGKSASRLAAQTVRVLPRKRLSRALGMLASARAPKPVVDAAIAA
                     FVRAYDVDLSEVDMPPGGFRTFDEFFTRTLLDGARPVDRDPGVLVSPADGRIEDLGEI
                     TLEGELIVKGQPYTAAALLGDSDAAAMYQGGHYFIVYLSPRDYHRVHAPTCGSVQYTR
                     YIPGSLFPVNRIGTEFIPQLFARNERLAIVQEGVVHGVVTTIMVGAIGVGRIGLSFDD
                     VQTNRGDTPGVRSYVEAPIPRDRGDELGVFHMGSTAIVMTPPRCKLEILVRAGTTIRM
                     GEAMARGGVDER"
     gene            3740..5164
                     /locus_tag="VFG22_08695"
     CDS             3740..5164
                     /locus_tag="VFG22_08695"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6416363.1"
                     /translation="MSGESADKPGGLQSHAPRRRRALRVPVTDIPRASVLDESKHSLE
                     NGGGNGAGYLTSNVVELDPPSGITTALDASDLYDDRDPTEPSFLTEPDPLGGDGPIVD
                     AADLSEPSIDVHFSDPPAIHPSTTPPASRRAGYSEPPEELFLDDADVLSRSDIAPPPD
                     RAAEMMIPIPKPPPPPAESADAGPPEVEADEDGPWYEHFFGEAYLRTVRASTPKEVAA
                     ECDFIEKALRIPVGSRVLDVGCGLGVQTVELASRGYALVGLDISPTMVSRAYDEAEDH
                     GLHIDFVRGDMRDVTFEEQFDALLCWGTSFGYFNEEENELTLRQFHQALKPNGTLLLE
                     VVNRDFMIGSQPNQVWFEVDGAVCMEETDFDYVTSRLRVKRRVASHSGQQNDRHYSIR
                     LYALHEIRGLLERNGFRVDEISGREATQGIFFGVHSPKMIIRAERLAGLFSEDGNTPP
                     PMPTPPIPKGALRPSEFPSDPVDD"
     gene            complement(5173..5769)
                     /locus_tag="VFG22_08700"
     CDS             complement(5173..5769)
                     /locus_tag="VFG22_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416364.1"
                     /translation="MRWMLFGLPWLALGCWGQTEEPPGEAVGTFNAVGFMVEQSCGEA
                     IPAPDPLDLDFELRSESNGRAYWRRLGGSMFAGVEKDGEFTFQVSQSWMVIQPDRFRG
                     YVGCSVTQRDVFTFDVEPVEVGADAGVEDGGANDAGSDLDAGTEPNLLTLSGSQTTEI
                     VPIAGSDCTPAVAALGGPFLSLPCRVEYVLTGTGVAIE"
     gene            complement(5860..6681)
                     /locus_tag="VFG22_08705"
     CDS             complement(5860..6681)
                     /locus_tag="VFG22_08705"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HET6416365.1"
                     /translation="MSNPLGNLPPWALETGWVVLAIATHAALLVFMPKVPAGPGFEDP
                     LDAVAVAIVDEDPLAEEPESPADDEASQPEEAPEPEPVKPPPKPKTFKAPEEPVEPEE
                     EPPAAAPETPVAFDNIVLTNEGDEPASWAMDPGSGESSDKPIGRPDAVVSGRSRDGIV
                     GGVIGGTGTGVVVDVGDLSKQPIPPSLKSKLERLYPKKARQDGIEGEAVIRLQIAPDG
                     LPSKIRIISESPGGYELGKACMKTLQGERWQPPIDKSGKPVTTRVTYRCGFEIRY"
     gene            complement(6702..7121)
                     /locus_tag="VFG22_08710"
     CDS             complement(6702..7121)
                     /locus_tag="VFG22_08710"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET6416366.1"
                     /translation="MAGGARSGDDDDLITDINVTPLVDVVLVLLIILMVTATAIVSKT
                     IPMELPKAATGEQTPSTLAVSIDQSGQVFLDTIPVTTDELRTRVRAARETDEDIRAVI
                     AADGRIAHAKVVQVIDILRQERITKFAINVRPEEIGR"
     gene            complement(7124..7768)
                     /locus_tag="VFG22_08715"
     CDS             complement(7124..7768)
                     /locus_tag="VFG22_08715"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET6416367.1"
                     /translation="MIDIQERLTAFAMLGATWVMWLLIFLSIIGVAIIFERIYYFFAT
                     RDDVRKLQEDLRLLLAKNDVPGARERLAQSKSFQARIVYSGIEVVDDGSAAVEERLAG
                     ETAVARTQMERNLGFLGTVGNNAPFVGLLGTVIGVIKAFHVLDESAGKVTSGLMTEVG
                     EALVATAIGLLVALPAVAFYNYFQRLIRYRLTWSDALGRDLLAYLKGNSSKGKG"
     gene            complement(7795..8832)
                     /locus_tag="VFG22_08720"
     CDS             complement(7795..8832)
                     /locus_tag="VFG22_08720"
                     /inference="COORDINATES: protein motif:HMM:NF013322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HET6416368.1"
                     /translation="MTLPYLTASIDPIPGELKTTPEDFEVEEVPAYAPSGDGEHVFAL
                     IEKRGLTTRDAVRALCELVGADPNAAGWAGLKDKHALTRQWISIWGATPESVKAAEIE
                     GVRVLEAAQHPQKIRTGHLRANRFRIRLRQVDTERIDDVRKVLSEIETQGLPNYYGEQ
                     RFGREGDNAERALRWVRGEARMPRSGFQRKIQISALQSEIFNRRVAERVETSTLAKVF
                     SGDLMKKHESGGVFVASYLDEVEARVNAWEISPTGPIFGAKMRWPEGEALAREQALLE
                     EAGLTVAQLAKWKRLAPGTRRFVRVPVPQITLGVSDNTVTLEFTLPAGSYATILIREI
                     LKRDAQPPKTS"
     gene            8886..>9848
                     /locus_tag="VFG22_08725"
     CDS             8886..>9848
                     /locus_tag="VFG22_08725"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET6416369.1"
                     /translation="MAEDPRTRIERILGRELEAACSDGVFPGASAAVAVWLGASWSYI
                     EASAGVRFEGAERVGPDTVYDLASLTKPLVANAALRLHQAGVFDLAERVDVLVPEADG
                     LPIGERTWEEVLSHRSGLAAWAPFYETLPAAPGSEEAGAWILAELLPRWERSQVGKAV
                     YSDLGYILAGIGMTRATDQALSQIVEERVTAPLGIDTELFFGADRQDDGWKARSAPTG
                     WSPWRQRILVAEVHDDNCAALGGVAGHAGMFGDAKSVARFGAACVGGWHGRRGAADEE
                     LVRHSTAVRAGGTLRLGWDGKAPEGSAAGTSIDADAFGHLGFTGT"
BASE COUNT         1777 a         3054 c         3136 g         1881 t
ORIGIN      
        1 gaaggcgaag tcctcgctga tcacggcgcg gactctagca cggtcagtct cagtcgagcg
       61 tgttcactgc agcgggatag gcgtccacca tcaggcagcg gctcgcccgg accgacggtg
      121 caccggtcat cactcagacc ctgccgaaga cagtattcgt cgcgcgcaat ccttgcgtct
      181 ccgcaaactt gtcgtctaca acgcaagcgt gagcttcgtt tgggtggcca tcgggggagc
      241 ggcaggatcc tgtcttcgat tttgggtttc attgatcctc ggacccgtca cggctgcgca
      301 cggttttccc tgggcgacgc tcgcggcgaa cgtgcttggc tcgctcggcc tcggtttggt
      361 gttcgtcctc ggcgaggggc gctcatggtt cgggggtgac ctgcgcttgc ttcttggaac
      421 cggtgtcatg ggcggcttca cgacgtactc ggcgttcaac ctcgagagca ttgggctcat
      481 gcaagatggc gcctggggtc gcgccggact gtacatgggc ggcacgatgc tgctctgcct
      541 actggcgggc gcgctcggcg tgatcgtcgg tcgcgcgatc cgcggatgac tcagggctcg
      601 ggcttgccta agagccagac acgcgcggtg tgcaaagttg cgtagagggc gcccgcgccg
      661 gcggcgaggc ctccgagcca gtagctgccc tgtgagagca agatcgcgcc cgcgatgatc
      721 aaagagccga cgacgaggcc gctgaacgca cgcctgccca agcggtcggc agcagactcg
      781 agctgcgtct gctgggtacg aacggtgaat gcgccgctcc gaaggtcctc gaggatctct
      841 tcgagttgca tcggcacctc ggaagtcgca acgctcagcc gccgcaggcc acgaagcgca
      901 tcttgggtca tgcgttccgg tgagtaacgc tgctgaaaga gcttcatgaa gtacgggcgc
      961 acctcttcga gcaggtcgag ctccggatag atctccttgc ccaccccttc aacggtcatc
     1021 aggctcttac cgagcatcag aaactccgag gggatctcta cgccgaactt tcgtgagccc
     1081 atgatcaggt cgcgaaggag agggccgagc tcgatatcct tgagccgctt gccgaggtag
     1141 cgctgcgcca aggtcgcgac ctcggcgtcg taggcatcgc ggttgatctt gtgctgcggc
     1201 cggcctatcg cgaagagcgc gtctgcaatg ccgccataat cctcacgcac cgcggcgagc
     1261 atcaagtcga tggtccggtc gcgcattttg ggcgcaagcc ggccgaccat gcccacgtcg
     1321 atcatgccga tgatcgggtc gtcgtcggcg ccgaggatga tgatgtttcc agggtgcggg
     1381 tcggcgtgaa agaatccgtc ttcgaagatc tgctggatga tgatctccac cgaacgcttg
     1441 gcgatgatct ctccgctggc acctgcacaa accgcggcat cgaccttgcg accctccagg
     1501 tactcgagcg tgagaacacg cttggcgctc gcttcccggt ataccttggg aaacttcgcg
     1561 tggggattgt tccggaaatt ctccgtgaag cgctcgtgat tgtccgcctc gagcgagaaa
     1621 tcgagctcag ccgacaccga acggtcgaac tcctcgacga gactcaccgg acggtaaagg
     1681 tgggactccg gcatcgaccg aacgattgcc ttcgccagcc agtagagaag gtcgacgtcc
     1741 cgggccatca cggtttggac ctcgggtcgc tggaccttca ccaccacctc tgcaacgtct
     1801 tcaccgtttc gaagccgcgc gcggtgcacc tgcgccaccg acgcggaggc caggggcgcc
     1861 tcctcgaacg actggtagac ttcggacagc gaagcgccga gctcgcgttc cacctgctga
     1921 cgaatgacgt cgaagggttc ggggggtacg tcgtcttgga gcttcttgag ctcgacgagg
     1981 atgtcctccg ggatgaggtc gggccgcgtg gacatcatct gcccaagctt cacgaaggaa
     2041 ggcccgaggt cctccaggac cttgcgcagg cggatcccgg ccggcattct gccgccatcg
     2101 ccgcgctttc gcccagggat cagcgtgctg agccccgtac ggtccaccac ctcgccgaat
     2161 ccatgaacgg cgagcacccc ggcgatctgt cgaagccgct cgaggtcgcg gacagtcgtg
     2221 aggagagaga ccatgggtca gaatcctagc atcgccgact gcgacccttc ctagatcgcc
     2281 tagccgcact cgtatttacg gccatgaaac aattccgaaa taatcggggg gtacatccat
     2341 tcgcatcggc ggttctcgtt gggctgcttg aagtttcgga ggtcccatga agaaggtgga
     2401 agccattatt aagcctttca agctcgatga ggtgaaggat gccctcaacg aggtaggcat
     2461 caaaggcatg accgtcacgg aggtcaaagg gtttggccgt acgggcggca agcgagaggt
     2521 ttatcgagga tccgcatacg tggtcgactt cgtccccaag gtgaaggtgg agatcgtcgt
     2581 cccggattcg ctggtggtct cggtaatcga agcgatcgaa aatgccgcga aaaccggacg
     2641 catcggcgac ggtaagattt tcgtttcgcc cattgaggag tctgtccgaa tccggaccgg
     2701 cgagcgcgga gaagcagcgg tctagtcgac caagcgatcg tgagaacctc gctgggcctg
     2761 acatcccggc gaggtcttcc tctgctgcag atgaaaatca tcgggatttc ggcaacttga
     2821 ccgagtgcag aggtttgacg cggcctcgtc cggtcggata ccctttcgtg acagcaccgg
     2881 ttcatgggga agagtgcttc acgactagcc gcgcagacgg ttcgtgttct gccgcgcaag
     2941 cggctgagca gggcgcttgg gatgcttgcg agcgcccgag ctccaaagcc tgtcgtcgat
     3001 gcggccatcg cggccttcgt gcgcgcgtac gacgtggatc tgagcgaggt ggacatgccg
     3061 cctggcggat tccgcacgtt tgacgagttc ttcacaagga cgctgctcga cggtgcgcgt
     3121 cctgtcgacc gcgatcccgg ggttctggtg tcgcccgccg acggacgaat cgaggatctc
     3181 ggcgaaatca cgctcgaagg cgagttgatc gtgaagggcc agccgtacac cgccgcggcg
     3241 ctgctcggcg actccgacgc ggccgccatg taccagggcg gtcactactt catcgtctac
     3301 ctatctccac gcgactacca tcgcgtacac gcgccgacct gcggaagcgt gcagtacacg
     3361 cggtacatac cgggatcatt gttcccggtg aaccgaattg ggacggagtt tatccctcag
     3421 cttttcgctc ggaacgaacg tcttgctatt gttcaagagg gcgtggttca cggagtcgtg
     3481 acgacgatca tggtgggggc gatcggtgtg gggcgtatcg gcttgagctt cgatgatgtg
     3541 cagacgaatc ggggggacac tcctggcgtg cgcagctacg tcgaggcgcc gattccgcgc
     3601 gaccggggcg atgagctcgg cgtgttccac atgggctcga cggcgatcgt gatgacgcca
     3661 ccgcgctgca agctcgagat cctcgtccga gcaggaacga cgatccgcat gggcgaggcg
     3721 atggctagag ggggcgtcga tgagcggtga atccgccgac aagcccgggg gccttcagtc
     3781 gcatgcgccg cgtcgccgca gggcacttcg ggttccagtc accgacatcc cacgcgcgag
     3841 cgtcctcgat gagtcgaagc actcgctcga gaacggcggc ggcaatgggg ctggatacct
     3901 gacatcaaac gtcgtggagc tcgacccccc cagcgggatc acgactgcgc tcgatgcatc
     3961 ggacctctat gatgaccgag atccgaccga gccatcgttc ctgaccgagc ccgatcctct
     4021 cggcggtgat ggaccgattg tcgatgcggc cgacctgtcc gagccgtcca tcgacgtgca
     4081 cttcagcgat cctcccgcga ttcatccgtc gactacacct ccggcgagcc ggagagctgg
     4141 gtacagcgag ccacctgaag agctctttct cgatgacgcg gacgttctat ctcgcagcga
     4201 catcgccccg ccgccggaca gggccgccga gatgatgatt ccaattccaa agcctccgcc
     4261 gccgcctgca gaaagcgcgg acgccgggcc gcccgaggtc gaggcggacg aggatggacc
     4321 ctggtacgag cacttctttg gagaggccta tctccgcacg gttcgtgctt cgacgccaaa
     4381 agaggtggcc gccgagtgcg atttcatcga aaaagccctc cgaattccgg tcggctcacg
     4441 cgttctcgac gtgggctgcg ggctcggcgt tcaaaccgtc gagctggctt cgcgaggcta
     4501 cgctctagtg ggcctcgata tttcgccaac gatggtctcg cgcgcctacg acgaagcgga
     4561 agatcatggt ctgcacatcg atttcgtgcg tggtgacatg cgggatgtga cgtttgaaga
     4621 gcaattcgac gcactgctgt gctggggcac ttcgttcggc tacttcaatg aagaggagaa
     4681 cgagctcaca cttcgtcagt tccatcaggc gctcaagcca aacggtacgc tcttgctgga
     4741 ggtcgtcaat cgcgacttca tgatcgggtc gcagcccaat caagtttggt tcgaggtcga
     4801 cggcgccgtg tgcatggagg aaacagactt cgactacgtg acctcacgtc tccgcgtgaa
     4861 gcgccgtgtt gcaagccaca gcggccagca gaacgaccgt cactactcga ttcgcctgta
     4921 tgccctgcac gagatccgcg gactgctcga gcgcaacggc tttagggtgg acgagatcag
     4981 cggccgcgag gcgactcagg ggatcttctt cggcgttcac tccccgaaaa tgatcatccg
     5041 cgccgaacga ttggccgggc tgttttccga agatggcaac acacctccac ccatgccaac
     5101 gcctccgatt cccaagggtg cgttgaggcc gtctgaattc ccgtccgatc cggtcgacga
     5161 ttagggctcg ggctactcga tcgcgacgcc ggtcccggtg agcacgtact cgacgcggca
     5221 aggcagcgaa aggaaaggac cacccagcgc ggcgaccgcg ggcgtgcagt ccgagccggc
     5281 aatgggaacg atctcggtgg tctgtgaacc tgacagagtc agcagatttg gctcagtccc
     5341 agcgtcgaga tcggatcctg cgtcgttcgc cccgccgtcc tcgacccccg cgtcggcgcc
     5401 cacctcgacg ggctcgacgt cgaaggtgaa gacatctcgc tgcgtgaccg agcagccgac
     5461 gtagccgcga aacctatcgg gttgaatgac catccagctc tggctgactt ggaacgtgaa
     5521 ctccccgtct ttctcgacac cggcaaacat cgagcctccg agacggcgcc agtaggcccg
     5581 gccgttcgat tcgctccgga gctcgaaatc gaggtccagc ggatcaggcg cagggatcgc
     5641 ctcgccgcag ctctgctcca ccatgaagcc gaccgcgttg aacgtgccca ccgcttcgcc
     5701 gggcggttcc tcggtctgcc cccagcaacc gagcgcaagc caaggcaggc cgaatagcat
     5761 ccaccgcatt caagccaggt accgccacaa ctgctcgggc gtcaaagaaa ctgctgcgag
     5821 ggacggcccc gaaaaggcgg cccaaagcaa cgaaagacct cagtagcgga tctcgaagcc
     5881 gcagcggtag gtgactcggg tcgtgaccgg tttaccgctc ttgtcgatgg gcggctgcca
     5941 gcgctcgcct tgcagcgtct tcatgcaggc tttgccaagc tcatacccgc ccgggctttc
     6001 cgagatgatc cggatcttgc ttggaaggcc gtccggggcg atctgcagac ggatcacggc
     6061 ctcgccttcg atgccgtcct ggcgagcctt cttgggatag agcctttcta gctttgactt
     6121 caaagatggc ggaattggtt gctttgaaag atcacccaca tcgacgacaa ccccggttcc
     6181 agtgcctcca atgacgccgc ccacgatacc gtcgcggctc cttccgctga ccacggcgtc
     6241 cggtctgccg atcggcttgt cgctgctctc gcccgagccc ggatccatcg cccaagacgc
     6301 cggttcgtca ccctcattgg tgagcacgat gttgtcgaag gccactggcg tttcaggggc
     6361 cgcggccggg ggctcctcct cgggttcgac gggctcctcg ggcgccttga acgtctttgg
     6421 cttcggtggg ggcttcacgg gttccggctc gggagcttct tcgggctgcg aggcctcgtc
     6481 gtcggcgggc gactccggct cctcggcaag gggatcctca tcgacgatcg cgacggccac
     6541 ggcatcgagc gggtcttcga aaccgggtcc cgcgggcacc ttgggcatga agactagaag
     6601 cgccgcatgt gtcgcgatgg ccagcacgac ccatcctgtt tcgagtgccc aaggcggcag
     6661 attcccgagc ggattggaca tcgtatcgcg cgccgcgctg ctcagcgtcc aatctcctcg
     6721 ggacgcacgt tgatggcgaa tttggtgatc cgttcttggc gcaagatgtc gatcacctga
     6781 acgaccttcg cgtgcgcgat tcggccgtct gcggcgatca cggctcggat atcctcgtcg
     6841 gtctcacgcg ccgcacgaac ccgggtccgg agctcgtcgg tggtaaccgg aatggtgtcg
     6901 agaaacacct ggccgctctg atcgatcgag acggcgagcg tcgaaggagt ctgttcgccc
     6961 gtcgccgcct tgggaagctc catcgggatc gtcttcgaga cgattgcggt tgcggtcacc
     7021 atgaggatga tgagcaacac gaggaccacg tcgacgagcg gcgtgacgtt gatgtccgta
     7081 atcagatcgt cgtcgtcgcc cgatcgtgcg ccgccagcca tgactagcct ttccccttgg
     7141 agctgttccc tttgagatag gcgagcaggt cgcgtccgag cgcatccgac caagtgagcc
     7201 gataacggat gagcctttga aagtagttgt agaaggccac ggcgggaagg gcgacgagaa
     7261 ggcctatcgc agttgcgacc agcgcttcgc cgacctcggt catcaggccg ctcgtcactt
     7321 tgccggcgct ctcgtcgagc acgtggaacg ccttgatgac accgatgacc gtcccgagga
     7381 ggcccacgaa cggagcgttg ttgccgaccg ttccgaggaa cccgaggtta cgctccatct
     7441 gggttcgcgc gacggccgtt tctccggcga gacgctcttc gacggcagcg gacccatcgt
     7501 cgaccacttc gattcccgag tagacgattc tggcctggaa cgacttcgac tgcgccaggc
     7561 gctcacgtgc tccgggaacg tcgtttttgg ccaggagcag ccgcagatcc tcctggagct
     7621 ttcggacgtc gtcgcgggtt gcgaagaagt agtagatccg ctcgaaaatg atcgcaacgc
     7681 cgatgatgga gaggaatatc agaagccaca tgacccaggt cgcgccgagc atggcgaagg
     7741 ctgtgagtct ttcttgaatg tcgatcatgc ttttcctcgg gtagaagcgc gcttttagct
     7801 ggtcttgggc ggctgcgcat cccgcttgag aatctcccga atcaatatgg tcgcgtagga
     7861 gccagcggga agggtgaatt cgagggtcac ggtgttgtca ctcacaccga gcgtgatttg
     7921 cggaaccgga actcgcacga aacgcctggt tccgggggct agtcgcttcc acttggcgag
     7981 ctgcgcgacg gttaatccgg cttcctccaa gagggcctgt tcccgagcga gcgcctctcc
     8041 ttctggccag cgcatcttgg cgccgaaaat cggacccgtt gggctgattt cccaggcatt
     8101 cacccgcgcc tcaacttcgt ccaggtatga cgcgacgaag accccgccgc tctcgtgctt
     8161 cttcatcaaa tcccctgaga aaaccttcgc gagcgtcgag gtttccacgc gctccgcaac
     8221 gcggcgattg aagatctcgg attggagagc cgagatctgg atctttcgtt ggaatccact
     8281 gcgtggcatg cgggcttcgc cccggaccca gcgaagagcc cgttccgcat tgtcgccttc
     8341 gcgcccgaag cgctgctctc cgtaatagtt gggcaaaccc tgggtttcga tctccgacaa
     8401 cactttgcgt acgtcgtcta ttcgctccgt gtcgacttgc cgaaggcgaa tgcgaaaccg
     8461 attggcgcgc aggtgtccgg ttcgaatttt ctgcggatgc tgcgcagcct ccaaaacgcg
     8521 cacgccttcg atctccgctg ccttcacgga ctccggcgtc gcgccccaga tgctgatcca
     8581 ttggcgcgtg agcgcgtgct tgtctttgag ccctgcccag cccgcggcgt tcggatcggc
     8641 gccgacgagc tcgcatagag ctcgcaccgc atccctcgtt gtgaggccac gtttctcgat
     8701 gagcgcaaat acgtgttcgc cgtcgccgct cggcgcgtac gcgggaactt cctcgacctc
     8761 gaaatcctcg ggagtcgtct tgagctcgcc tgggatgggg tcgatcgagg cagtgaggta
     8821 aggaagcgtc atcggggccg gcgcttcctg tagaccattg cagaccgatt gggtagacag
     8881 cagtcgtggc cgaagacccc aggacccgca tcgagcgaat tttggggcgg gagctcgagg
     8941 ccgcttgcag cgatggggtc tttcccggtg caagcgcagc ggttgctgtc tggctcggag
     9001 cgtcctggtc gtacatcgaa gcatcggccg gcgttcggtt cgagggtgcg gagagggtcg
     9061 gtccggacac ggtctatgat ctggcctcac tcaccaagcc cttggtcgcc aacgctgcgt
     9121 tgcgattgca tcaggccggc gtcttcgatc tcgcggagcg cgtggacgtc ctggttccag
     9181 aggcagatgg gcttcccatc ggagagcgca cttgggaaga ggtcttgagc caccgctcgg
     9241 ggctcgcggc ctgggccccg ttctatgaaa cgcttcccgc agcgcccgga tccgaggaag
     9301 ccggcgcctg gatcctcgcg gagcttctcc cccgctggga acgcagtcag gtcggcaagg
     9361 ccgtgtacag cgaccttggc tacatcctgg cgggcattgg aatgactcgc gccaccgatc
     9421 aagccttgag ccaaatcgta gaggagcgag tcacagcgcc gctcggcatc gacacggagc
     9481 tattcttcgg agccgatcgg caagacgacg gatggaaagc acgctcagcg ccgaccggct
     9541 ggtccccgtg gcgacaaaga atcctcgttg cggaagtgca cgacgacaac tgtgccgcgc
     9601 tcggcggcgt ggcagggcat gcaggcatgt ttggtgacgc gaagagcgtc gctaggtttg
     9661 gagccgcgtg tgtcggaggc tggcacggca gacgcggggc cgccgatgag gagctggttc
     9721 ggcattctac ggccgtccga gccggcggca cgctgaggct cggctgggac ggcaaggccc
     9781 cggagggcag cgccgcgggc acgtcgatcg acgccgacgc gttcgggcac ctcggcttca
     9841 ccggtact
//
LOCUS       DASRZF010000196         8590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2541934, whole genome shotgun sequence.
ACCESSION   DASRZF010000196 DASRZF010000000
VERSION     DASRZF010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 8590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8590
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2541934"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="VFG22_08730"
     CDS             <1..786
                     /locus_tag="VFG22_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416370.1"
                     /translation="LALRFGWLFRFKPQLQFGVMLQPPGIPLKQRVGAVSQEFVNDEQ
                     DPSSPLTTTVAYLNDDFDANLPIPLELRTGVMYWPAEKVMLALDAMFYGPVRSGQRVK
                     NGSSGLVGGLFYDNNTARRAIGNVAVGADFFISKKIMIETGFFTDFSSAIKIPDNPDR
                     YYNPQVNRYAGTISIGLDVAGVAFAVGTTFLYGKGPATGAVIDQNDLALGYTRTEATT
                     RTIFLHLTGATRAVSTLSDKTAKGIKTHRDKKQNGDKQTQGED"
     gene            complement(783..1856)
                     /locus_tag="VFG22_08735"
     CDS             complement(783..1856)
                     /locus_tag="VFG22_08735"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6416371.1"
                     /translation="MNLFQRIEVGVSERLLRASDQFVERFTKDRWEVDGELFDRRAQY
                     VFTLGRWVERSMPAMTPPDARSYYARLCRILESAPPEVGGTEELTIPMRSGPRPARIY
                     YPRGADETTESRPALVYYHGGGWTIGSIDTHDTLCRRLCSNARIVVVSVDYRLAPEDP
                     FPAAVEDCYDAYLWVSDQAPALGICANRIAVGGDSAGGNLAAVVTALARDKGKPLPSV
                     QLLIYPGVGTVHHIGRQKAELQTGYGLDDKTTRWFFANYVPEGEEENPLVAPLHLASH
                     AGLSPAIVVTAQFDLLCGEGLEYVERLEAAGVPVTHLHQTDLPHGFATMSVLPRAREA
                     VSELAGALGRALRHGREEARTLG"
     gene            2014..2096
                     /locus_tag="VFG22_08740"
     tRNA            2014..2096
                     /locus_tag="VFG22_08740"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2048..2050,aa:Leu,seq:tag)
     gene            2355..3593
                     /locus_tag="VFG22_08745"
     CDS             2355..3593
                     /locus_tag="VFG22_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HET6416372.1"
                     /translation="MLNIAALFLVITALLAYLNHRFVRMPTTIGVMSAALVLSLSLVG
                     LSALGVAESLLQYEQSLLRSIDFSQVLMQGMLSLLLFAGALHVDLSELKTYRLPVILL
                     AVLGTLLSTLLVGYGMWLVLPLVGLEIPLMYCLCFGALISPTDPIAVMGILKSAGAPK
                     ELELVIAGESLFNDGVGVVIFSLLLEMLTSGAAPTPAHGLQLLLHEAGGGLLFGFALG
                     YVTFRLLKSVDNYQVEVLLTLATVTGGYALASQLHVSGPLAMVVAGLMIGNHGRALAM
                     SDTTRHYLDMFWELVDEILNAVLFVLIGMEVLLVVFSASVLVAAGLAVAVTLLARLVT
                     VGLPVRLLRGAFKLPHGSWKVLTWGGLRGGISVALALSLPLGADRDTVVSLTYGVVVF
                     SILVQGLSIGYVTRRSVASV"
     gene            complement(3900..6035)
                     /locus_tag="VFG22_08750"
     CDS             complement(3900..6035)
                     /locus_tag="VFG22_08750"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HET6416373.1"
                     /translation="MGQTQLASWHRDEHGIPQIVADDINGLYWGMGYCHAMDRGLQML
                     IMRILGQGRAAELLDGSDEMVQVDRFFRRMNWNGGVAEEVENLSAEARAAVEAYCSGV
                     NARFAESSPWELKLVGAKPEPWAPEDSLLLARMTGFLTLAQSQGEVERLFVEMVQAGV
                     SDAHLEALFPNSTAGLDRAMVSQVQLGERMVPDSVKWVAAASRMTASNNWCVSGSRTA
                     SGAAMLANDPHLETNRLPNVWVEQSFRWRDSHAILMTMPGLAAPLIGRNEHLSWGATY
                     TFMDAIDSWVEHCRDGKYRRGEQWVDFERRVETIRRKKREPITETFYENLHGVLDGDP
                     TDEGYLLATRWSGSFGGAQSINSLLRMWTAKTVPEGMVAYAQVESSFNWIFADSAGDI
                     GYQMSGLMPVRPNDWNGFAPRPGWDQRFDWRGFVVQSLLPRVHNPESGMVVTANQDLN
                     HLGEADPINAPMSDYRARRIEQLLAARDDHDVDSFRAIQLDTHSIQAAEFMEILQPLL
                     GTGPIADALRAWDYRYDLDSVGATAFEIFYAALLVEMFGPGCGTAVIEHLRDATGTFI
                     DFHQNFDRLLVSESSPWLDGRTRDEVWRAALEKVARQVPEPWGQRNRVSLTNMFFGGK
                     LPRFLGFDPGPIPIRGGRATPHQGQIYQSGGRLTSFAPSLRLVTDMSDQSLHTAICGG
                     PSDRRFSKWYKSGVEGWLEGSLKVRKPPT"
     gene            6056..6334
                     /locus_tag="VFG22_08755"
     CDS             6056..6334
                     /locus_tag="VFG22_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416374.1"
                     /translation="MANGGERFDGVTTGRSEPALNEEVGMVVAQACDGNHLHGRTIEL
                     GRLLGYCHRHRHPPTGAPQLRMSVIRVLPDGDGLDTSFGDEGVARLMP"
     gene            6492..7103
                     /locus_tag="VFG22_08760"
     CDS             6492..7103
                     /locus_tag="VFG22_08760"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HET6416375.1"
                     /translation="MNEARRSSPLPDEVQWLYRRLMNKDHLAQARAQWSHRGSGRPEW
                     AIDPGENQESVWDYPRPPRLDLDSRLVQVVHEDEVIAESRRAIRVLETASPPTFYIPP
                     QDVRTELLTRAPGASLCEWKGSATYWSLCLDGHEIRNAAWSYEQPFVDFASLRGYLSF
                     YPGKLECYVGEDRVTAQPGGFYGGWVTPEIVGPFKGEPGTGGW"
     gene            complement(7134..8300)
                     /locus_tag="VFG22_08765"
     CDS             complement(7134..8300)
                     /locus_tag="VFG22_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416376.1"
                     /translation="MHLNRFAICVLALTSVLGCSSGGGEADNGGTGGSDVDFTSFEWT
                     ELNGDAPWSRRAGLHTVELAGRFYLMGGRTPTDPMGAPFPPADFWADVWVSDDEGVTW
                     EKILSNDAPGHWAPRAYFQAVVKGDAMYVLGGQDFGFPTSNFYSDVWSSEDGIAWTER
                     TADAGWEPRAGLRAVTFQNEIYIFGGSQFDDNAILPGPSGPARIYYNDVWKSADGAQW
                     QEVTANAPWEPRAGAVVVVKDDYMYLLGGEAGFLCDTNADPDCELPYFNDVWRSQDGA
                     DWELVTDSAGWSPRPGHQCAVSFDHIVCWGGFGLPENPMDVWVSPDGATWEQVSDSPW
                     NAESPDEIKYDFEGIAVGDVIYTFGGDRETFDFSDPSNYLRVDNDVWRYALPAP"
BASE COUNT         1553 a         2658 c         2728 g         1651 t
ORIGIN      
        1 ttagcgcttc gcttcggctg gctctttcga ttcaaaccgc agctccagtt cggcgtgatg
       61 ctgcagcctc cgggaatccc gctcaagcaa cgcgttggcg cggtctcgca agagttcgtc
      121 aatgacgagc aggacccaag ttcgcctctc acgaccacgg tcgcctatct aaacgacgac
      181 ttcgacgcga atctgccgat ccctctggag ctccgaaccg gtgtcatgta ttggccggct
      241 gagaaggtca tgttggcctt ggacgcaatg ttctacggcc ccgtgcgctc ggggcagcgc
      301 gtgaaaaacg gctcgtcggg actggtcggg ggcctgttct acgacaacaa taccgcgcgc
      361 cgcgccatcg ggaacgtcgc ggtcggcgcg gatttcttca tcagcaagaa gatcatgatc
      421 gagacgggct tcttcactga cttttcctcg gcgatcaaga tccctgataa tccagatcgg
      481 tactacaatc cgcaagtcaa tcggtacgcg ggcaccatat cgatcggact tgacgtcgct
      541 ggtgtcgcgt tcgcagtggg aaccaccttc ctctatggaa agggaccggc gacgggcgcg
      601 gtcatcgatc agaacgacct ggccctcggg tacacgcgca ccgaagccac cacccgcacc
      661 atcttcctgc acctcacggg cgctacccgg gccgtttcaa ccctcagcga caagacggcc
      721 aagggtatca agacgcatcg cgacaagaag cagaacggag ataaacagac acagggcgaa
      781 gattagccaa gggttctggc ctcttcgcgt ccgtggcgca gcgcgcgacc gagcgctccg
      841 gcaagctccg aaacggcttc gcgcgcgcgg ggcaggacgc tcatcgtggc gaagccgtgc
      901 ggcaggtctg tttggtgcag gtgtgtcacc gggacgccgg cggcctcgag ccgctccaca
      961 tattccaggc cttcgccaca gagcagatcg aattgcgctg tgaccacgat ggctgggctg
     1021 agtcccgcgt gggaagctag atgaaggggg gcgacgagag gattctcctc ttcgccttcg
     1081 ggcacgtagt ttgcgaagaa ccagcgcgtt gttttgtcgt ccagcccata gccggtttgg
     1141 agctcggcct tctggcggcc gatgtgatgg acggtgccga cacctggata gatcagaagc
     1201 tggacgctcg gtaggggttt tcctttgtcc cgcgcgagcg cggtgaccac tgctgccagg
     1261 tttccgccgg cgctgtctcc gcctacggcg atccggtttg cgcagatccc cagggcaggg
     1321 gcctgatcgg agacccagag gtaggcgtcg tagcaatcct cgacggcagc ggggaagggg
     1381 tcttcgggcg ccaagcggta gtcgacggag accacgacga tccgtgcatt gctgcagagg
     1441 cgccgacaga gtgtgtcgtg cgtatcgatg cttccgatcg tccacccgcc gccatggtag
     1501 tagacgagcg cgggccggct ctcggtcgtt tcgtctgcgc cgcgagggta gtaaatgcga
     1561 gcggggcgcg gaccggagcg catcgggatc gtcagctctt ccgtgccacc gacttctggc
     1621 ggggcggact cgagaattcg gcagaggcgc gcgtagtagc ttcgtgcgtc cgggggcgtc
     1681 atcgccggca tcgagcgctc gacccacctt ccgagcgtga acacgtactg ggcgcgccgg
     1741 tcgaacagct cgccgtcgac ctcccaccgg tccttcgtga agcgctcgac gaactgatcc
     1801 gaagcacgaa gaaggcgttc tgagacgcca acttcgattc gctgaaagag gttcatgctc
     1861 cgcgatccgg aagtcgtccg tttgtcatgt gccgcccaaa actaaaggca ggagctgcag
     1921 ggcgcaatcg gaggccagaa gacccatggc gcgcgtcttc gcgaggctgg ccaggtcccg
     1981 tgaaggtgct aatccccgaa acctgtgcac cgagcgaggg tggcggaatt ggcagacgcg
     2041 ctggatttag gttccagtgt ccttagggcg tgcaggttcg agtcctgtcc ctcgcactgg
     2101 tgtgatgtcg gggggttagc gcgggcggcg ttcttcgctc cctcgaaacg tcttttgatc
     2161 tgtcccccta ctggtggatg cctgaatacg acgagcaacg gccccacgat gcactcggtg
     2221 acctgacccc gacggaagcc cgccaaaaaa ccgccagaat ctctacttac aaactgccgc
     2281 cttgacgggg aagcttacga ctgcgccaca gtcacgtacg ctccgtctgg cccagtacaa
     2341 cctcgaccga ttctatgctc aatattgccg ctctctttct cgtaattaca gcgctgcttg
     2401 cctacctgaa ccaccgtttc gtccgcatgc caacgaccat cggtgtaatg agcgccgccc
     2461 tggtcctgtc gctatcgcta gtcgggctga gcgccttggg cgtggcggag agcctgctgc
     2521 agtacgaaca atccttgctg cgctcgatcg atttttccca ggtactgatg cagggtatgc
     2581 tctcgctgct gctctttgcc ggcgcgctgc acgtcgatct gagcgagctc aagacgtacc
     2641 gccttccggt cattctgctg gccgtgctgg gaacgctgtt gtctaccctc ttggtgggct
     2701 acggaatgtg gcttgtgctg ccgttggtcg gtttagagat acctctgatg tattgcctgt
     2761 gtttcggcgc gctgatctcg ccaaccgacc cgatcgctgt catgggtatt ctcaagtccg
     2821 ccggcgcacc aaaggaactg gagctggtga tcgcaggcga gtcgcttttc aatgatggcg
     2881 tgggtgtcgt gatcttctcg ctgttgctcg agatgctgac gagcggcgcc gcgccgacgc
     2941 cggctcatgg tctgcaactg ctgcttcatg aggccggtgg cggcctgctg ttcggctttg
     3001 cactgggtta tgtgaccttt cgacttctga agagcgtgga caattaccag gtcgaagtgc
     3061 tgctgacgct ggccacggtc acaggcggct atgcgctcgc gagccagctg cacgtgtccg
     3121 gcccgctggc gatggtggtc gcaggcctga tgatcggtaa ccacggtcgt gcactggcca
     3181 tgtccgatac gacgcggcac tacctcgaca tgttctggga gctagtcgac gagatactca
     3241 acgcagtgct gttcgttctg atcggcatgg aagtactgct ggtcgtcttc tcggccagcg
     3301 tgctggtcgc cgcaggcctg gccgtggccg tgacgctgct tgcccgactg gtcacggtcg
     3361 ggctaccggt gcggttgctg agaggggcat tcaagctgcc acacgggtca tggaaagtgc
     3421 tcacctgggg cggcctgcgt gggggcattt cggtggcact tgccctgtca ctacccctgg
     3481 gcgccgaccg ggataccgtc gtctcattga cttacggcgt cgtcgtcttc tccatactcg
     3541 tgcagggact ctcgatcggc tacgtcacgc gccgatcggt tgcgagcgtg taacgggcgg
     3601 cgccgacggt aagcgcccgg ccgtcgaagg ctgcgcagga tgagcaggca gtacacgaag
     3661 gtggcgacga aggtgccgag caccacctgg ttggcggtat cccgcatgaa gttgcgcagc
     3721 aagcggggac ccagctgaga gaaggcaacc gcagacaccg cagctctgaa gcgtacgcag
     3781 cggccaactt ctctggcgcc gtgtacgctc ataccgactg tcgccggccg ctcgcgtcga
     3841 caccgccagg gcctttgttt tgcctatctg ccgaagctca tgccatgcgg ctagattggc
     3901 taggttggcg gctttcggac tttgagagag ccctcgagcc agccctccac gccactcttg
     3961 taccatttcg agaaccgccg atcggacggg ccgccgcaga tggcggtgtg caggctctgg
     4021 tcgctcatgt cagtcacgag ccggagcgaa ggcgcgaacg aggtgagccg accgcccgac
     4081 tggtagatct gaccttggtg cggggtcgcg cgtccgccgc gaattgggat tgggcctgga
     4141 tcgaagccaa gaaagcgggg aagcttgccg ccaaagaaca tgttcgtgag cgacacgcgg
     4201 ttgcgctggc cccacggctc ggggacctgt cgagcgacct tttcgagcgc tgcgcgccat
     4261 acctcatcgc gcgtgcgtcc gtcgagccaa ggcgaggatt cggagacgag gagacgatcg
     4321 aagttttggt ggaagtcgat gaatgtaccg gtcgcgtcac ggagatgctc gatgacggcg
     4381 gtaccgcacc cggggccgaa catctcgacg agaagcgccg cgtagaagat ctcgaacgcg
     4441 gtcgctccca cggaatcgag atcataccgg tagtcccagg cccgcaaggc gtcggcgatg
     4501 gggcccgttc cgagcaaggg ctggaggatc tccatgaact cggcggcctg gatcgaatgg
     4561 gtgtccagct gaatcgcgcg aaacgagtcg acatcgtggt cgtcgcgtgc cgccaggagc
     4621 tgttcgatac gccgcgcgcg atagtcactc attggcgcgt tgatcgggtc cgcctcgccg
     4681 aggtggttga ggtcttggtt cgcggtcacg accatcccgc tctcggggtt gtgcacgcga
     4741 gggagcaggc tttgaacgac gaagccgcgc cagtcgaatc gctggtccca gccggggcga
     4801 ggagcgaatc cattccagtc gttcggccgc accggcatca aacccgacat ctgatatccg
     4861 atgtcaccgg cgctgtccgc gaagatccaa ttgaacgacg actcgacctg ggcgtaagcg
     4921 accatgccct ccggcaccgt cttggccgtc cacatccgga gcagtgagtt gatcgattgg
     4981 gcgccgccga acgagcccga ccatcgtgtg gcgagcagat acccttcgtc ggtgggatcg
     5041 ccatcgagta cgccgtgcag gttctcgtag aacgtctcgg taatcggctc gcgcttcttt
     5101 cgccgaatcg tctccacgcg ccgctcgaaa tcgacccact gctcaccgcg ccggtacttg
     5161 ccgtcacggc aatgctcgac ccaggagtcg atcgcatcca tgaatgtgta ggtcgcgccc
     5221 cacgacaggt gctcattgcg cccgatcagc ggtgcagcca ggccgggcat ggtcattaga
     5281 atcgcgtggc tatcccgcca tcgaaagctc tgctcgaccc acacgttggg gagccggttg
     5341 gtctcgagat gcggatcgtt cgcaagcatg gcggcgcccg acgcggttcg cgaccccgac
     5401 acacaccagt tgttggacgc cgtcatgcgt gatgcggcgg ccacccattt gacggaatcc
     5461 ggcaccatgc gctccccgag ctgcacttgg gacaccatcg cgcgatctag accggctgtg
     5521 ctgttcggga agagcgcctc gaggtgcgca tcgctgaccc ccgcttgaac catctccacg
     5581 aagagacgct ccacttctcc ctgcgactgt gcgagcgtga gaaagccggt cattcgcgcg
     5641 agcaagaggc tgtcctctgg cgcccagggc tcgggctttg ccccgaccag cttcagctcc
     5701 cagggactgg actccgcgaa acgtgcattc acgccgctgc agtacgcctc gacggcggcg
     5761 cgcgcctcgg cgctgaggtt ttccacttcc tctgcaacac cgccattcca attcatgcga
     5821 cgaaagaaac ggtcgacctg gaccatctcg tcgctgccat cgaggagctc ggcggcgcgc
     5881 ccctgcccga ggatccgcat gatgagcatc tgcagcccgc gatccatcgc gtggcagtag
     5941 cccatgcccc aatagaggcc gttgatgtca tccgcgacga tctgcggaat gccatgctcg
     6001 tcgcgatgcc agctggctag ttgcgtctgt cccatgacca gcagttgtcc tgggcttggc
     6061 gaatggtggc gaacgattcg acggggtcac gaccggccgc agcgagccgg ccctgaacga
     6121 agaagtgggc atggtggtcg ctcaggcatg tgatgggaat cacctgcatg gccggacgat
     6181 agaactcgga cgcctgcttg gctactgtca tcgccaccgc catccgccga ccggcgcgcc
     6241 gcagctccgg atgagcgtga tccgtgtcct tccggacggc gacggcctcg acacgagctt
     6301 tggcgacgaa ggcgttgccc gactcatgcc ctgaggagtt cgaattgaac gctgatctac
     6361 tcagggaggg aagggcaagg cggcttcgca agcctggatc gcagcttcgt tcggcagcac
     6421 cgagggagcg agatctgggg gctctgtgaa ggccgaccgt gatctgcgcc atcttcgagt
     6481 agcctcgcct tgtgaacgaa gcacgccgat cctcaccact tccggacgag gtccagtggc
     6541 tctatcgtcg tctgatgaac aaggaccacc tagcacaggc acgtgcacaa tggagccatc
     6601 gtggctcggg gcggccggaa tgggccatcg atccggggga gaaccaggag tcggtttggg
     6661 actatcctcg tccgcctcga ctcgatctcg actcacgtct cgtgcaggtc gtgcatgaag
     6721 acgaagtgat tgcagaatcg cgtcgcgcga ttcgcgtgct cgagaccgct agcccaccta
     6781 ccttctacat cccgccgcaa gacgtccgga cggagctgtt gacacgagcc ccgggcgcct
     6841 cgctctgcga atggaagggg tctgcaacat actggtcgct atgcctcgat gggcatgaga
     6901 tccgcaacgc ggcttggagc tacgagcaac cgttcgtgga cttcgcttcg ctccgcggat
     6961 acctgagctt ctatccgggc aagctcgagt gctacgttgg cgaggatcgc gtgaccgcgc
     7021 aacctggtgg cttctacggc ggttgggtga cgcctgagat tgtcggcccg ttcaaaggag
     7081 agcctggcac cgggggttgg tgagcaaggt ctgcgacgag tcggtttctt cggtcagggc
     7141 gcgggaagtg cgtatctcca gacatcgttg tcgacgcgga ggtaattgct ggggtcgctg
     7201 aagtcgaaag tctcacggtc gccgccgaac gtgtagatca cgtctccaac cgcgatgccc
     7261 tcgaagtcgt acttgatctc gtcggggctc tccgcgttcc aaggggaatc gctgacctgc
     7321 tcccaggtcg ccccgtcggg gctgacccac acgtccatcg ggttctcggg aagcccaaag
     7381 ccgccccagc aaacgatgtg gtcgaacgaa accgcgcact ggtggccagg ccgcggtgac
     7441 cagccggccg aatcggtgac gagctcccaa tccgcgccgt cttgcgaacg ccagacgtcg
     7501 ttgaagtagg gcagctcgca atcggggtcg gcgttggtgt cgcagaggaa gccggcctcg
     7561 ccgccgagca agtacatgta gtcgtctttc accaccacga cggcgcccgc gcgaggctcc
     7621 catggtgcat ttgcggtcac ttcttgccac tgagcgccgt cagccgactt ccagacgtcg
     7681 ttgtagtaga tccgggctgg gccagaaggt ccgggcaaga tcgcgttgtc gtcgaactgt
     7741 gatccgccga aaatgtagat ctcgttttgg aaagtcaccg cccgaagacc cgcgcgaggc
     7801 tcccagcctg cgtcggcggt acgctcggtc catgcgatac cgtcttcact ggaccagacg
     7861 tcgctgtaga agttgctcgt cggaaaacca aagtcctgac cacctagaac atacatcgcg
     7921 tcgcctttga cgacggcttg gaaatacgcg cgcggggccc aatgaccggg cgcgtcgttc
     7981 gagagaatct tctcccaggt gacgccctcg tcatcgctca cccagacgtc tgcccaaaaa
     8041 tcggcaggtg gaaacggggc gcccatcggg tcggtcgggg tgcgcccgcc catcaggtag
     8101 aacctgccag caagctcgac cgtgtgaaga ccggcgcgcc gagaccaggg cgcatcaccg
     8161 ttgagctcgg tccattcgaa gctcgtgaag tcgacgtcgg agccgccggt tcctccgtta
     8221 tcggcctctc cgccgccact gctgcagccg agcactgagg tcaaagcgag aacgcaaatc
     8281 gcgaatcggt tgaggtgcat cgtggtactt gtatcataga gatccggagg cgactgcttt
     8341 caattatacc cggagcgccc gcctcagcgt tgtatgccgc gcccgtgcgg ttactatttc
     8401 caaactcctg ctagaagtgg acctgatggg cgcaccgaag gaaatccgca cagggcggcc
     8461 gcaccgtccg ttgggtgtga gcttgatcaa tcagtttggc cgactggcca atgcgatggg
     8521 gatgcatccg gtcacgctcg atccggatcg actcgtcgcg aaggcgattc agaaggccgg
     8581 cagcgcggac
//
LOCUS       DASRZF010000197        10156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_480094, whole genome shotgun sequence.
ACCESSION   DASRZF010000197 DASRZF010000000
VERSION     DASRZF010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 10156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10156
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480094"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..814
                     /locus_tag="VFG22_08770"
     CDS             143..814
                     /locus_tag="VFG22_08770"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HET6416377.1"
                     /translation="MHDLDRAIRERRSVRGFRPEPVPREVLEEVFELAQRAPSNCNVQ
                     PWRVYVALGERVEALRRALVEAVSAGEASAAMAPVDEFRDGYREKQIACAVELYGKMG
                     IERGDRAGRSMAMLRNFEFFDAPHVAYVCMAKSFGIGVALDVGMYVQTLMLAMQSRGI
                     GSCAQAALRAYPSLVARQLGIPEDEQILCGLSFGYEDESVAANRTRQPRDPISNNVFF
                     RGDAG"
     gene            complement(811..1599)
                     /locus_tag="VFG22_08775"
     CDS             complement(811..1599)
                     /locus_tag="VFG22_08775"
                     /inference="COORDINATES: protein motif:HMM:NF005126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HET6416378.1"
                     /translation="MTEERPRVLTERDGHIFHIVLDRGDKMNAFDLRMLRELAEAVTE
                     YESDESLWCALLYANGEHFTAGLDLAEVGPAVRSGQPLFPEGSIDVLSLHEPKRKKPL
                     VMAVQGWCLTIGIELLLAADIRLAAEGARFGQIEINRGIFPFGGATIRLPEVAGWGNA
                     MRWLLTGDRFDSEEALRIGLVQEVVPANELRAKAIEIARTVAKRAPLGVQATIRSSRT
                     AQLEGHDAAIAELLVIARELMDTEDAAEGIRSFIERREGDFKGR"
     gene            complement(1596..2063)
                     /locus_tag="VFG22_08780"
     CDS             complement(1596..2063)
                     /locus_tag="VFG22_08780"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HET6416379.1"
                     /translation="MSIELLIAIGLPLLLIVVGRVAGSRIERSHYESILEREARFSKQ
                     PAFSIKQIDMPGTIGSASLAIGSVVVSVDHFKRFVSGFRMLIGGEVRAYSSLIERARR
                     EAVLRMKESQPNADAYINTRIETSTISSTAGNEGMGTIEVLAYGTAVHYDRAP"
     gene            complement(2071..2385)
                     /locus_tag="VFG22_08785"
     CDS             complement(2071..2385)
                     /locus_tag="VFG22_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HET6416380.1"
                     /translation="MILTNIDSVPGRRIVEHFGLVQGSTIRAKHFGRDFAAGLKNIVG
                     GELKGYTELLQESRAEATSRMVTQAQELGANAVINVRFTTSSVAQGASEILAYGTAVW
                     IE"
     gene            complement(2432..3748)
                     /locus_tag="VFG22_08790"
     CDS             complement(2432..3748)
                     /locus_tag="VFG22_08790"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6416381.1"
                     /translation="MSAVRVHYLNHASLLVDVGKVRMLFDPWLEGTAFSDGWGLRYEN
                     PEAFALGAKATHLWISHWHSDHLHAPSLSKLAELNPEMVVLANVSANFSMEQRLASFG
                     FRDVRPIRERETLHLDAETSCCRYPTAGIDNMLHIRSGAWSMVNYNDCNLPINAIRSL
                     RKQIGPIDILFTNYNHAGKLFDSLDDAARKQQLIDTLELTVNAIHPTHVVPFASSHYY
                     RAEESADQNSSMLDFSELEALSKTDDRYLVLRIGDEAELRSQTTPLVRRREPPIAPRE
                     LETLRYSSSVPWEQLLAEVSVRANALQSQFLHMARLAVPDLIVRVSDHGRCLRLAMGA
                     NATEAEASAEPQIEAHSQALRTWLARRFGDDTFIAGAHFRVLQPDTSVVERWALVTLL
                     EASHLDPQRFLRYLGEPEGRRFLWNRREEILATVLGRRWTAGRMRI"
     gene            complement(3761..5377)
                     /locus_tag="VFG22_08795"
     CDS             complement(3761..5377)
                     /locus_tag="VFG22_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416382.1"
                     /translation="MKGQFVPERASVAVLAVACLAHYWICSSLSSEIALDVDPINLVY
                     GMREFNIAHYAPHPPGYLVYVWMLRALHAVVGGTPLGTVQLLARVLSTLTIPLVFMAV
                     TTLRPTDPVARGYAAVLTAFHPFLIYHAVDAQTHTSEAFAAALLLLALVHYLGRPTII
                     WAAAMGAALALGSAFRPSFVVAGIGPIIWAIGFRRIAHLSVAGISSLLGAFSWMMPTF
                     QASGGFAQWRAANDALIQQTIVRTSSPFSDQAIAGFVAFNMRSTALWLLLALAPAFVA
                     MLSRLGSAAPPDPAYTRARSIAFWSAGPAVLFYLAMFCSEPGYLLGFVPAIIAVTALA
                     ASPKLSHARRRFALIAALATQLGILMLPPASSQVAKVPSVPELVRREVLYRTVFERMR
                     DELPRGARALYVSDYQDVVLSRQLPVLSPNVHSLVYHREYEPPYQQTSISFATADDWI
                     PIPGPILLQPGPPSVQAMPYTYDFIVVDPIASADLRKELSRASSCDLRVPEGAVDVLV
                     VPAERCFPEGVIASHGRGIRFSLASAPDPG"
     gene            5487..6803
                     /locus_tag="VFG22_08800"
     CDS             5487..6803
                     /locus_tag="VFG22_08800"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HET6416383.1"
                     /translation="MKLCVIGTGYVGLVTGTCFAATGQRVVCVDKDASKVERLQRYEI
                     PIYEPGLEEMVKENASAGRLTFTTDLAAAVNDVDLAFIAVGTPPTHDGEADLSAVFAV
                     AQELAKHATQDLVIVNKSTVPVGTNAQVQKLVADAKHNIQVVSNPEFLKEGGAINDFM
                     YPDRIVIGVRPGHDRARRLMERVYHPLNLSSTKIVWMNPESAEVTKYAANTMLAMRIS
                     FMNELAVLCEEVGADVQSVRKGVGSDPRIGSQFLHAGPGYGGSCFPKDVKALVATARR
                     HGIELELADATNRVNVRHRSFLVRKLKRHFGSDLAGKRIAIWGLAFKPGTDDIRESPS
                     ITSIEFLLNEGCEIVAHDPEAMGNIREVFGESVALVDDAYDAVKNADALMLLTEWREY
                     QYPEFDRIREMMRTPVILDGRNIWVTYKLGEQGFEYAGVGIRTDPV"
     gene            6810..7433
                     /locus_tag="VFG22_08805"
     CDS             6810..7433
                     /locus_tag="VFG22_08805"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HET6416384.1"
                     /translation="MCGRFTLTAADEETLIGGLPFDTFSETRIQFRPRYNIAPGQRSP
                     IVHFESGKPVLLDALWGLDRAGSGLVVNARSETADRTALFREAFREGRCLVPADGFFE
                     WRREGAVNQPYLFQREDGGLLLMAGLRRGERFVVLTEESKGVVAEIHDRMPVLLEGER
                     ARDWLGEGTLVSAPRLKRTAVSRRVNRIENDDPDCLSPLAQSSFEFD"
     gene            complement(7443..8060)
                     /locus_tag="VFG22_08810"
     CDS             complement(7443..8060)
                     /locus_tag="VFG22_08810"
                     /inference="COORDINATES: protein motif:HMM:NF024636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HET6416385.1"
                     /translation="MKQYSRDEVEEILRRALESQPVEALSHEDLVAAAVEAGIDAADV
                     ESAARQLEEERELLLEEDRIVSARKKRFLQSVYTYVVVNAGLFVIDIMSGPGWWVQWI
                     LAGWGIALALGARRALAPDRQRLRARARRRMQKRQRGNWEKQVRKEVDRAIQLGVDAL
                     VDAASRKLSARAEESSRERPRVRVDRAAAETIDVEALENAKRGRS"
     gene            complement(8063..9805)
                     /locus_tag="VFG22_08815"
     CDS             complement(8063..9805)
                     /locus_tag="VFG22_08815"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6416386.1"
                     /translation="MADKETTSFMRSLCMGRIEQDVIFPFPTLSDAERETLADISGAL
                     EGLLGPRAEDFRKWDVAGELPEEFIEELKEFGMFGLVIPESYGGMGLGNKAYSRTLQE
                     VAKYDASVAVTIGAHSSIGMRGLLLFGTEDQNNRYMEKLASGEMIAAYCLTEPGAGSD
                     AAAVKTTAVKEGDHWVINGNKLWITNGGIADFFTVFAKTPEEGGRGKMTGFIVTKDME
                     GVSIGPHEDKMGLRSSSTTTVHFDNVKVPNENVLGEVGQGFKVAMAILNSGRTGLGGG
                     SVGAMKNLIKMATVQANERKTFGQPISSYGLIKKKVGEMVIDCYTSEAAVTVVGGLID
                     EGYKEYQVEAAISKVYATECLWRTADEALQIAGGNGYMREYPYERIVRDCRINRIFEG
                     TNEILRLLIALTAMNDVASQLQELSATMKDIFNEPIKGFGLLSDYARKHAALRTGIRG
                     EAKFSGLDPIIHEQAQIFEESTRFLAQATDRILRKHGKGIIGKQFASHRLAEIMIDLF
                     VMACTLSRVQASIDAKGVDAAALEIDILRTFTRAAKVRIKRNFRRIDSNDDEMLKSLA
                     EDAFKAEGFRWDTL"
BASE COUNT         1931 a         3053 c         3239 g         1933 t
ORIGIN      
        1 tggggtcgga gttcgtgcgc agtggggtga tcgagcccgc gctccgaaaa gtggtgggag
       61 aggtacgcgc aatccaaatc gaaaaagcgg tcccaaggcc gaagcggcgt gcctaagagg
      121 atcgaggcgc tcaggggtga cgatgcatga tctagaccga gcgattcgcg agcgaagatc
      181 cgtccgtggg ttcaggccag aaccggtgcc tcgcgaggtc ctcgaagagg tgttcgagct
      241 cgctcagagg gctccgagca actgcaacgt tcagccatgg cgcgtctacg tggcgctcgg
      301 agagcgggtc gaggcgcttc gccgcgcgct cgtcgaggca gtgagcgcgg gcgaggcttc
      361 ggctgccatg gcgcccgtgg acgagtttcg cgacggctac cgtgaaaagc agatcgcctg
      421 cgcggtcgag ctctacggca agatggggat cgaacgcggc gacagagcgg ggcgctcgat
      481 ggccatgctg cgcaacttcg agttcttcga tgcacctcac gtggcctacg tgtgcatggc
      541 gaagagcttt ggtattggag ttgccctaga cgtcgggatg tacgtgcaga cgctgatgct
      601 cgcgatgcag tcgcgaggaa tcgggtcctg cgcgcaggcc gcgttgcgcg cctatccgag
      661 cctggttgca aggcagctgg gcatccctga agacgagcag atcctctgtg ggctgtcttt
      721 cggctacgaa gacgagtccg ttgcggccaa ccgaaccagg cagccccgcg atccgatttc
      781 gaacaacgtg ttcttccgag gcgacgcggg ctaacggccc ttgaaatcgc cttcgcgacg
      841 ctcgatgaac gagcggatgc cctccgcagc gtcttcggtg tccatcagct cgcgggcgat
      901 cacgagtaac tcggcgatcg cagcgtcgtg gccctcgagc tgcgcagtgc gcgaggagcg
      961 aatcgtcgcc tgcacaccga ggggtgcgcg cttcgcgaca gtacgcgcga tttcaatcgc
     1021 cttggcgcgg agctcgttcg cgggaaccac ttcttgaacg aggccgattc tgagggcctc
     1081 ttcggaatcg aagcgatctc ctgtgagaag ccatcgcatg gcgtttcccc agcccgcgac
     1141 ctcggggagg cgaatggttg cgccgccgaa ggggaagatc ccgcgattga tctcgatctg
     1201 accgaagcgg gctccttctg ccgcgaggcg aatgtcggcc gcaagcagaa gctcgatgcc
     1261 gatcgtgagg caccagcctt gcacggccat gaccagcggt ttcttccgct ttggctcatg
     1321 aaggctcagg acgtcgatag aaccctcggg gaacaaaggc tggccgctgc gaaccgccgg
     1381 acccacctct gccaggtcga ggcctgcggt gaagtgctcg ccgttggcgt agagcagggc
     1441 acaccagagg gactcgtctg actcgtactc ggtgacggcc tctgcgagct ctcggagcat
     1501 gcggagatcg aaggcgttca tcttgtcgcc gcgatcgaga acgatgtgaa atatgtgtcc
     1561 gtcgcgttcg gttagcaccc gggggcgttc ttcggtcatg gggcgcgatc gtagtggacg
     1621 gcggttccgt aggccaggac ttcgatggtg cccatcccct cgttgcccgc agtgctggaa
     1681 atggtcgacg tctcgatgcg ggtattgatg taggcgtcgg cattcggctg agattccttc
     1741 atgcggagga ccgcctcacg cctcgcgcgc tcgatgagcg aggagtacgc gcggacctcg
     1801 cccccgatca gcatccgaaa gccactgacg aagcgtttga agtggtcgac cgaaaccacc
     1861 acggacccaa tggcgaggct cgccgagccg atggtacctg gcatgtcgat ttgtttgatg
     1921 gagaacgcgg gttgcttgct gaaccgtgct tcgcgttcga ggatgctctc gtagtgactg
     1981 cgttcgatgc gcgatccggc gacccggccg actacgatga gtagcaaggg taagccgatc
     2041 gcgatcagta gctcgatgga catggcacgc ctactcgatc catacggcgg ttccgtaggc
     2101 caagatctcc gaggctcctt gagccacgga ggaagtggtg aaacgcacgt tgatgaccgc
     2161 gttggcgcca agctcctgcg cctgggttac catccggctc gttgcttccg cgcggctttc
     2221 ttggagaagc tcggtatagc ccttcagctc tccgcccacg atgttcttga gcccggcggc
     2281 gaaatctctt ccgaagtgct tcgcccgaat ggtgcttcct tgcaccaagc cgaagtgctc
     2341 cacgattcga cgccctggca cgctgtcgat gtttgtcaga atcactcggc ccccttgaac
     2401 aattgttgta gcagaaagcg ctggctcgat tctagattcg catgcgtccg gccgtccagc
     2461 gccggccgag gaccgttgcg aggatctctt cgcggcgatt ccagaggaag cgtcgcccct
     2521 ccggctctcc gaggtatcgc aggaaacgct gagggtcgag gtgactggct tcgagcaagg
     2581 tcaccagcgc ccatcgctcg acgaccgaag tgtcgggctg cagcacacga aagtgggccc
     2641 ccgcgatgaa tgtgtcatcg ccgaagcgac gggcgagcca ggttcgtagg gcttgcgagt
     2701 gcgcttcgat ctgcggctca gctgaggctt cggcctcggt cgcgttggca cccatcgcca
     2761 gacgcaggca gcggccatgg tcgctcacac gcacgatcaa atccggcacc gcgagcctgg
     2821 ccatgtgcag gaactgactc tgcagcgcat tcgctcggac tgaaacttcg gcgagcaatt
     2881 gctcccaagg caccgacgac gagtaccgga gtgtctcgag ctctcgtggg gcgataggcg
     2941 gctcgcgccg acgaaccagc ggagtggtct gagaacggag ctcggcctcg tcgccgatcc
     3001 gcagcaccaa gtatcgatca tcggtcttgg agagcgcttc gagctccgag aagtcgagca
     3061 tcgaagagtt ctgatcagcg ctttcctccg cccggtagta gtggctcgat gcgaaaggca
     3121 ccacgtgagt cgggtgaatc gcgttgactg tcagttcgag cgtgtcgatg agttgctgtt
     3181 tgcgagcggc atcgtcgagc gagtcgaaca gcttgcctgc atgattgtag ttcgtgaaca
     3241 gaatgtcgat tggcccgatc tgcttgcgga gggagcgaat cgcgttgatg gggaggttgc
     3301 agtcgttgta attgaccatg gaccaggcgc ccgacctgat gtggagcatg ttgtcgatgc
     3361 ctgcggttgg ataccggcag cagctcgttt ccgcgtcgag gtgcagagtc tctcgctccc
     3421 ggatcgggcg tacgtcgcga aagccaaagc tcgccaagcg ctgctccatg gagaagtttg
     3481 ccgacacatt ggccaaaacc accatctccg ggttcagctc tgcgagcttc gacaaggatg
     3541 gggcgtgcag atgatcgctg tgccaatgcg aaatccagag atgcgtcgcc tttgcgccga
     3601 gagcaaacgc ctcggggttc tcgtagcgaa gaccccagcc gtcggagaat gccgtgcctt
     3661 cgagccaggg atcgaagagc atgcgtacct tgccgacgtc gaccagaagc gaagcatggt
     3721 tgagatagtg aacgcgaacc gcagacatca cgacttcctt tcagcctgga tcgggcgcgg
     3781 atgccagtga aaaacgaatg ccccgtccgt gcgacgcgat tacgccctca ggaaagcagc
     3841 gctccgcggg gacgaccaga acgtcgacgg cgccttcggg gactcttaga tcacagctcg
     3901 aggctcggct cagctccttg cggaggtctg cactcgcgat cgggtcgacc acgatgaaat
     3961 cgtaggtgta gggcatcgcc tgaacagaag ggggacccgg ctggaggagg atgggtccgg
     4021 gaatggggat ccaatcgtct gcggtggcga aactgatcga ggtctgctgg tagggtggct
     4081 cgtattccct gtgatagacc aacgaatgca cgttcgggct gagcaccggg agctgccgac
     4141 tcaagaccac gtcttgatag tcggaaacgt acagcgcccg cgcgcccctc ggaagctcat
     4201 cccgcatgcg ttcgaatacg gtccggtaga gcacctctcg gcggacgagc tccggcaccg
     4261 atggcacctt ggcaacttgg ctcgaggcag gaggcagcat gaggattccg agctgcgtcg
     4321 ccaaggccgc aatcagagcg aagcggcgcc gtgcatgcga tagcttgggg ctggccgcga
     4381 gtgccgtgac ggcgatgatc gccgggacga agccgagtag atatcccggt tcagagcaga
     4441 acatcgcgag gtagaagaga actgccggcc cggcgctcca gaacgcgatc gagcgcgctc
     4501 tcgtgtatgc tgggtcgggc ggcgccgcgc ttccgaggcg actcagcatg gcgacgaatg
     4561 caggcgccag cgctaggagc aaccacaggg cagtgctcct catgttgaaa gcgacgaatc
     4621 ctgcgatcgc ctggtcgctg aacgggctgc tcgtgcgaac gatcgtttgc tgaatgagcg
     4681 cgtcgttcgc ggctcgccat tgcgcgaagc cgccgctcgc ttgaaaggtc ggcatcatcc
     4741 aggagaacgc cccaagcaga ctgctgatcc ctgcgacgct caaatgagcg attcgacgga
     4801 acccaatcgc ccaaatgata ggaccgattc ccgccacgac gaagctcggg cgaaaggcgc
     4861 tcccaagcgc caacgctgca cccatggccg ccgcccagat gatcgtggga cggcccaggt
     4921 agtggacgag cgccaagaga agaagagcgg cagcgaacgc ttcggaggtg tgggtctgcg
     4981 catcgactgc gtggtagatg aggaacgggt gaaacgcggt gagaacggcc gcataccctc
     5041 gggcgaccgg atcggttggc cggagtgtcg tgacggccat gaagacgagc ggaatggtga
     5101 gcgtcgagag gactcgcgcc aagagctgca ccgttccaag cggggttccc ccgaccacgg
     5161 cgtgcagcgc tcggagcatc cagacgtaaa ccagatagcc tggcgggtgt ggagcatagt
     5221 gcgcgatgtt gaactcacgc atgccataga cgagattgat cgggtcgacg tcgagtgcga
     5281 tctcggagct gagcgagctg cagatccaat agtgcgccag gcaggccaca gccagcaccg
     5341 cgacggacgc acgctccggc acaaactggc ctttcaagcc accgatcgcc tcccccccca
     5401 aatgctgccc tttcgtcgtc ccacaatacc gtctcacccc gagggtggca tctttcagga
     5461 agctggaatc gtgctatgcc ccgctcgtga aactctgcgt aattgggaca ggatacgtgg
     5521 gcctcgtcac cggtacctgc tttgcggcga ccggacagcg cgtggtttgc gtcgacaagg
     5581 atgccagcaa ggtcgaacga ctgcagcgct acgagattcc gatctacgag cccggtctcg
     5641 aggagatggt caaagagaac gcgagcgccg gccgtctcac tttcactacc gatctggcgg
     5701 ctgcagtgaa cgacgtcgat ctggcgttca tcgcggtagg cactccaccg acccatgacg
     5761 gggaagcgga tctaagtgcc gtgttcgcgg tcgctcagga gctcgcgaag catgcgactc
     5821 aggacctggt catcgtcaac aagagcacgg tccccgtggg cacgaacgct caagtccaga
     5881 agctcgttgc cgacgccaag cacaacattc aggtcgtctc caatccggag tttctaaaag
     5941 agggtggggc gattaacgat ttcatgtacc cggaccggat cgtcatcggc gtacgtcccg
     6001 ggcacgatcg cgcgcggcgc ctcatggagc gcgtgtacca cccgctcaac ctttcgagca
     6061 ccaagatcgt ctggatgaac cccgaaagcg ccgaggtgac caagtacgcg gcgaatacca
     6121 tgctcgcgat gcggatttca ttcatgaacg agcttgcggt tctctgtgaa gaagtcggcg
     6181 cggacgtcca gagcgtgcga aagggtgtcg ggagcgaccc tcgaatcgga tctcagttcc
     6241 ttcacgcggg gccaggatac ggcggctctt gctttccgaa ggacgtgaag gcgctggtcg
     6301 cgaccgcacg gcgacacgga atcgagctcg agctggccga cgcgacgaat cgcgtgaacg
     6361 tacgacatcg aagcttcctc gtgcgcaagc tcaaacggca tttcgggagc gatctcgctg
     6421 gaaaacgcat cgcgatttgg ggattggcat tcaagcccgg caccgacgac attcgcgagt
     6481 ctccctcgat taccagcatc gagttcttgc tcaacgaggg ctgcgagatc gtcgcacacg
     6541 atcccgaagc aatgggaaac atccgggaag tcttcggcga gagcgttgcg ctcgtcgacg
     6601 atgcgtatga cgcggtcaag aatgcggatg cgctcatgct tctgaccgaa tggcgcgaat
     6661 atcagtaccc ggagttcgac cggattcgcg agatgatgcg tacaccggtg atcctcgacg
     6721 gtcgcaacat ctgggtcacc tacaagctcg gcgagcaagg cttcgagtac gcgggtgtgg
     6781 ggattcgtac cgatccggtc tgagcgggga tgtgcgggcg attcacgctg accgcggctg
     6841 acgaagaaac gttgatcggc ggccttccgt tcgacacgtt cagcgaaacc cgcatccagt
     6901 ttcgtccgcg atacaacatc gcgccgggtc aacgcagccc aatcgttcat ttcgaaagtg
     6961 gcaagccggt tctgctcgac gcgctttggg gcctcgatcg cgccgggtca ggtctcgtgg
     7021 tcaatgcccg gtccgaaacg gcagaccgga cggcgctctt ccgcgaagcg ttccgtgaag
     7081 gaagatgcct ggtccctgca gacggattct ttgaatggcg aagggaaggg gcggtcaacc
     7141 agccctatct ctttcagcgc gaggacggcg gtctccttct catggcgggt cttcggcgcg
     7201 gcgagcgctt cgtcgtactt accgaagaat caaaaggagt agttgctgaa atccacgatc
     7261 gaatgccggt actactcgag ggagagcgcg cccgcgattg gctcggtgag ggcacgctcg
     7321 tttccgcgcc gagactgaag cgaaccgccg tttcacggcg agtcaaccgc atcgaaaacg
     7381 acgacccgga ttgtctcagt ccgctcgccc aatcgagctt cgagttcgac tgaagacctc
     7441 gactacgagc ggccgcgttt agcattctcg agggcttcga cgtcgatagt ctctgcggcg
     7501 gcacgatcta cccggacgcg gggacgctct cgggaagact cctcggcgcg cgcagagagc
     7561 ttgcgcgacg cagcatcgac gagcgcatcc acaccgagtt gaatcgcccg atcgacctct
     7621 tttcgaacct gcttttccca attgccgcgc tggcgcttct gcatgcggcg acgtgcccga
     7681 gcgcgaagcc gctgccggtc gggggccagg gcccggcgcg cgccgagcgc gagcgcgatg
     7741 ccccagccgg ccaggatcca ttgcacccac cagccaggcc cagacatgat gtcgatgacg
     7801 aagagaccgg cgttgaccac gacataggtg tagacgctct gaaggaatcg cttctttcgc
     7861 gcgctgacga tccgatcttc ctcgagcaag agctcacgct cttcttcgag ttggcgcgca
     7921 gcggactcca cgtcagccgc gtcgatgccg gcttccaccg ctgcggcgac cagatcctcg
     7981 tgcgagagcg cttctaccgg ttgggactcc aacgctcgcc gcaggatctc ttcgacctcg
     8041 tctcgcgagt actgtttcac ggctagagcg tgtcccagcg gaacccttca gctttgaatg
     8101 cgtcctcggc aagggacttc agcatttcgt cgtcgttgct gtcgatccgt cggaagttgc
     8161 gcttgatgcg caccttggct gcgcgcgtga aggttcggag aatgtcgatt tccagggcgg
     8221 cggcatcgac gcccttggcg tcgatcgagg cctgcacacg gctcagtgta caagccatca
     8281 cgaagaggtc gatcatgatc tccgcgagac ggtggctcgc gaactgcttt ccgatgatgc
     8341 cttttccgtg ctttcgaagg attcggtcgg ttgcctgcgc gaggaaccgg gtcgattctt
     8401 cgaagatttg cgcctgttcg tggatgatcg gatcaaggcc cgagaacttc gcttcgccgc
     8461 gaatcccggt tcggagggcg gcgtgtttgc gggcgtagtc ggagagaagg ccgaagccct
     8521 tgatcggctc gttgaagatg tccttcatcg tggccgagag ctcctggagc tggcttgcca
     8581 cgtcgttcat cgcggtgaga gcgatgagca agcgtaggat ttcattggtc ccctcgaaga
     8641 tccggttgat ccggcaatca cggacgatgc gctcgtaggg atactctcgc atgtatccgt
     8701 tgccgcccgc gatctgcagt gcttcgtcgg ctgtccgcca gaggcattcc gtggcgtaga
     8761 ccttcgagat cgcagcctcg acctggtact ccttgtaccc ctcgtcgatc aagccgccga
     8821 cgaccgtcac cgcggcttcg gacgtgtagc agtcgatcac catttcaccg accttcttct
     8881 tgatcaggcc gtagctcgag atgggctggc caaaggtctt gcgctcgttc gcctgaacgg
     8941 tggccatctt gatgaggttt ttcatcgcgc cgacggagcc gccgccaagg ccggtgcggc
     9001 cgctgttcaa gatggccatc gcgaccttga agccctggcc tacctcgccg agcacgttct
     9061 cgttcggcac cttcacgttg tcgaaatgga ccgtggtggt cgagctcgag cgtagcccca
     9121 tcttgtcttc atgaggtccg atgctcacgc cctccatgtc tttggtgacg atgaagccgg
     9181 tcatcttgcc gcgaccgcct tcctcgggcg tcttcgcaaa gacagtgaag aagtcggcga
     9241 tgccaccgtt ggtgatccac agcttgttgc cattgatgac ccagtgatcg ccttccttga
     9301 ccgccgtcgt cttcacggca gcggcgtccg agccagcgcc cggttcggtc aagcaataag
     9361 ccgcgatcat ctcgcccgag gcgagcttct ccatgtagcg gttgttctgg tcctcggtac
     9421 cgaagagcag cagtccgcgc atgccgatcg agctgtgcgc gccgatcgtc accgcgacgg
     9481 aagcgtcgta cttggcgacc tcttgaagcg ttcgagaata ggctttgttg ccgagcccca
     9541 taccgccgta cgactccggg atgaccaggc cgaacatccc gaactccttg agctcctcga
     9601 tgaactcctc tggcagctcc ccggcaacgt cccatttgcg gaagtcctct gcccgtggac
     9661 ccagtagccc ttcgagggca cccgagatgt cagccagcgt ctctctctcg gcatccgaca
     9721 gggtgggaaa ggggaagatg acgtcctgct cgatgcgccc catgcaaagg gatcgcatga
     9781 agctcgtggt ttctttgtcg gccactggtc gctccttttt cgaagggccc atcatgcacg
     9841 tcttgcttcc agccgtccat ggaaccctgg ctgagtctta gggccgtgcg gcggatcgcg
     9901 ccagcacgcg cgggcgcttc ctagggctca tccacgaaaa aaggccgccc gctttcgcag
     9961 gacggccttc ttccttgggg tgctcgaaga ttcgagcgcc ggactattca gccgaggcgg
    10021 tcgccgcgga gtccgacttg ttcttgttct tgcccatgac gccccagaag gcggttccag
    10081 cgccgatgcg ccaggcgaac gaagcgttgg tgttgctccc ggtgaccgtg atccccggac
    10141 ccagatcgaa gaagaa
//
LOCUS       DASRZF010000198        11434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2887348, whole genome shotgun sequence.
ACCESSION   DASRZF010000198 DASRZF010000000
VERSION     DASRZF010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 11434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11434
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2887348"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="VFG22_08820"
     CDS             <1..900
                     /locus_tag="VFG22_08820"
                     /inference="COORDINATES: protein motif:HMM:NF013260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6416387.1"
                     /translation="EAARRALEIAAAGSHHMLLIGSPGAGKTMLARALPSILPTPSAE
                     EALEIATIASSAGLRSLRILLGRQRPFRAPHHTASAAAIVGGGEPIRAGELTLAHRGV
                     LFLDELPEFRRDAIETLRTTMEEGEVVISRARQRVRLPAEPMVIAAMNPCPCGYHGDP
                     TRHCRCGELQIARYRGRVSGPLLDRFDLQVAVPRVSARVMRKATPGESSKAVQERVER
                     ARERLGITGARRSLESLTRNVAQEALTLLELGVERLKMSARAYLKALRVAHTIAVLDE
                     SSEVLPAHTAETLQYRVLDREPS"
     gene            954..1952
                     /gene="recA"
                     /locus_tag="VFG22_08825"
     CDS             954..1952
                     /gene="recA"
                     /locus_tag="VFG22_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HET6416388.1"
                     /translation="MKQALKVIDDLLQNVEKQFGKGAMMRLGDHKAERVPSIPTGSPS
                     LDEALGCGGYPKGRVVEVFGPESSGKTTLTLHAIAQCQAMGGVAAFIDAEHALDIHYA
                     KNLGVDAKELLVSQPDSGEQALDIAEILVKSGAIDLIVIDSVAALVPKAELEGDMGAS
                     HVGLQARLMSQALRKLAGAAHRTGTTIVFINQLRMKIGVMFGSPETTTGGHALKYYAT
                     VRLDVRRIGAVKQGDVVLGNRTRVKVVKNKLAPPFRKAEFDIRYGKGIDAIGDLLDQA
                     VEASIVEKSGSYYRFGGKNIAQGRESARDAMEKDSKLLEAIRIQLRPPAEDTSKAA"
     gene            1991..2944
                     /locus_tag="VFG22_08830"
     CDS             1991..2944
                     /locus_tag="VFG22_08830"
                     /inference="COORDINATES: protein motif:HMM:NF013255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET6416389.1"
                     /translation="MRCLVTGATGFIGGGVLRGLEADGHQVVAYVREGSDSRALHDVE
                     RVVGDLDDPSALARAASGCDAFVHAAGIADPAASVEALGWAHVAGTENALNAAKHAGC
                     RRFVHISCADVTLHDGPRSFWNEEAQPARPYGELARSKLQAEELVRVSGSKGFWTIAL
                     RPALVWGPGDTTHLPGWKAEAESGGIRIVGGGKKLLATTYIRNLAHAVLCALRTGVNT
                     GNVYYVVDTELSVSRELFTELSAALAWPPPKSAGPYRWAWFWSRTGLSSLHPAEVIRR
                     GQTSAFEMTKAKQDLEYEPIVTREQGLAELAAWFRETQAAR"
     gene            2941..3438
                     /gene="tadA"
                     /locus_tag="VFG22_08835"
     CDS             2941..3438
                     /gene="tadA"
                     /locus_tag="VFG22_08835"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HET6416390.1"
                     /translation="MTDDPSNENTDERWMRSAIAEARLAAAEGEVPVGAVIVHEGRII
                     ACAHNERESSQDPTRHAEMIAIQEAAKKLGSWRLIDTTLYVTLEPCPMCAGALVNARV
                     PRVVWGCDDPKAGATRTLYEIGTDARLNHRFECVPGVLDEACSALLTEFFGAIRAKSK
                     PRDSR"
     gene            3470..3559
                     /locus_tag="VFG22_08840"
     tRNA            3470..3559
                     /locus_tag="VFG22_08840"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3504..3506,aa:Ser,seq:cga)
     gene            3643..3727
                     /locus_tag="VFG22_08845"
     tRNA            3643..3727
                     /locus_tag="VFG22_08845"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3677..3679,aa:Ser,seq:gga)
     gene            3730..3820
                     /gene="ffs"
                     /locus_tag="VFG22_08850"
     ncRNA           3730..3820
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFG22_08850"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            3875..4663
                     /locus_tag="VFG22_08855"
     CDS             3875..4663
                     /locus_tag="VFG22_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416391.1"
                     /translation="MRFSIAFISLAMVIRPALALGQQTGAQPKDEPSSSIVVIDTPPP
                     QPSPAQPQPPSTGVILQRPGTVLLSGTPVRLKFRSPTDGVSFLLQSAGSYTSISGVTV
                     GMGWGGYGWGGWGWGGYGFGGMAPYYGEMVTQAYQPICAAPCDATLLSGRHRFALSLN
                     DGRPVNVPKALDLSVDSVVEGRYVDKSRMRKAGWATFVAGAVTGMALMFASINYHADP
                     FYGDQIRYRPMMFTGVGLFVGSIITGSVLASQNDEAQVIVYPED"
     gene            complement(4969..5505)
                     /locus_tag="VFG22_08860"
     CDS             complement(4969..5505)
                     /locus_tag="VFG22_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416392.1"
                     /translation="MEHSLSNTFRNASLVAIALLFALTTGCGDGLFQKSGGELSSSLS
                     RRIVERMGGTEVDLVELTGCEVLESNDPEARCEGVSDTQIEVFIDGESIIFDFSNVTK
                     GGKISASEFEGYLVSVTEHSRLPRILEAILDATQSTIDSQDLDIQFDDKNVAVNFQGL
                     DYDDATFVKIDLVFEDAI"
     gene            5731..6339
                     /locus_tag="VFG22_08865"
     CDS             5731..6339
                     /locus_tag="VFG22_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416393.1"
                     /translation="MTKYLLNAACLSLLSLMLVACGDAGVNQGDEDLNFDDPTVDEFV
                     DEEVVDEEIVDDELVDEEVVEELIAPFDNDSAANPAVDEFLSITNTREIVYTDQISAE
                     DGDNEDFVQFELPNNSNPNQRIEIEIDCDVFGDENVFARVELLNVDGNDTTRINGGPI
                     DCNEGPFQVTVRNDQVQLARIYVQGVPQEQTLIEYTLVVAPF"
     gene            complement(6427..6702)
                     /locus_tag="VFG22_08870"
     CDS             complement(6427..6702)
                     /locus_tag="VFG22_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416394.1"
                     /translation="MSDEKERAELLAALHAAGAASEEDEKELEALLAADPALAAVVAD
                     FQDSIMALASSFEPLETSPKTLGQIQKKIAKEKGGLLGRLRGWFSKL"
     gene            complement(6699..7283)
                     /locus_tag="VFG22_08875"
     CDS             complement(6699..7283)
                     /locus_tag="VFG22_08875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6416395.1"
                     /translation="MTDGRANDPAEFVAAMAAGDSGAPLSALYSLYASTVLALLTKML
                     GSRSEAEEILQEVFVELWRRAPQYDSSRGSVVAWVVTVARSRALDALRARSRRYGDKQ
                     MQFSDRHALGTDSGRPDVLVSSTRWHQALRQAFKQLSEDQRAVLELSYFLGMSHDQIA
                     ETLKLPVGTVKSRILAGMRALRTMLPIIHRSMVA"
     gene            complement(7280..8926)
                     /locus_tag="VFG22_08880"
     CDS             complement(7280..8926)
                     /locus_tag="VFG22_08880"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6416396.1"
                     /translation="MNPTPVIYNEGVLDTGVGQSANSREFLQQRVAAFGRTVAVLLFL
                     SLIGRIFLGVVFGYWQKEVAHPSFWTHAGAWLPVACIWLICRSRPLQTRTIQIVEGVG
                     LVLTSIALVVMGANLPAAAGAATTTAYALSFGLIARAIFVPSTAKHTAWLGLAIGIPL
                     VIAVYLNFLTVDLDVWNSHGFFRTIEKKQTLARSQAIQIGFAWALTTLLAAWATRVIY
                     GLRKDARDARKLGQYIVEAKLGEGGMGVVYRARHGLLKRPTAIKLLSADRNTPEDIDR
                     FRREVQMTAKLRHPNTITIYDYGCTEDGTFYYAMELLDGVTLLEIVEAEGTLPEPRVV
                     HIMRQCAMALHEAHAIGLVHRDIKPNNIMMTQQGGVHDVTKVLDFGLVKNVDSKVDMT
                     NTVSEAIIGTPRFMSPEQILDPPSVDARSDLYALGALGYYLLTGRFVFEGTSAIEICA
                     QHLSAIPDPPSSVAQRDINPGLERIIMQCLEKKRDDRPASAAEIAEELRSLPIERWSQ
                     PHASQWWEATGIELMMGRDRDSDVGSLATIQAEPLLTGTR"
     gene            9035..9211
                     /locus_tag="VFG22_08885"
     CDS             9035..9211
                     /locus_tag="VFG22_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416397.1"
                     /translation="MFTALRQPVVSNSVLNELATSGFFPDPALFVYGVLGALMVASLL
                     SAYLLFGRSDSIPS"
     gene            complement(9297..11207)
                     /locus_tag="VFG22_08890"
     CDS             complement(9297..11207)
                     /locus_tag="VFG22_08890"
                     /inference="COORDINATES: protein motif:HMM:NF015254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectin acetylesterase-family hydrolase"
                     /protein_id="HET6416398.1"
                     /translation="MRLTNLGLVALVAIPLVMGVGCSDTGSSGGTGGSGGDGGTGGSA
                     GTGGSAGTGGTGGDTTPESYAGNVCVGAKQAAASTFCKSMFDAWSAWEGDQDDDARGA
                     AEEAARTALGDAWDAAESDAQDASADCADLALTSSDAASGIEAQVTSIVSSINDGLDL
                     GQIDQATCGAAVLTAAGDACENVLAAESAYIGDLYADLDGSTLDAAKSAALDAFSSAW
                     ADATSENCPTSASEEEVRNDLQNLTDELVGNTIVAPSLDDQQYTTLTPGETEYLGRTY
                     TPKCMEASEYRYFAKRGSVNKLLMYYQGGGACWDNLTCGGTPLTGAICKTSTAEDDPN
                     SFSTGFANLSNENNPFRNWNIVFVSYCSCDVHFGDATQDYGDVTVEHKGYHNSKVAEK
                     WAREHFLNPEEVFVTGSSAGAYGAWFNAPLLHEVWPASQMHVLADAGNGVITPDFLQN
                     EFGNWNFVANLPDIPGVIEAITTGNGMPAYTEAVATEFPNTNWAHYSTKHDGGSGGQT
                     GFYNVMLTGSPLGALSWWDASCAFGDTALAQSMSTFDTVPSNYRYYFGTGSRHTMWGN
                     DKVYSDTTGGVPTVVDWVEGMLASGPDGRDPAWSNVECDNCGLLLAGDPRPDPLEAPF
                     EQQGEDVVIVCE"
BASE COUNT         2158 a         3660 c         3505 g         2111 t
ORIGIN      
        1 gaagcggcac gtcgtgctct cgagatcgcc gcagcaggca gccaccacat gctgctcatc
       61 ggaagcccgg gcgccggcaa gacgatgctc gctcgcgcgc ttccctccat cctgcccact
      121 ccttctgcag aagaggcgct cgagatcgcg acgattgcga gctccgccgg gcttcgatcg
      181 ctacggattc tgcttgggcg gcagcggcct ttccgcgcac ctcatcacac ggcaagcgcg
      241 gccgcgatcg tcggaggcgg cgagccgatt cgcgccggcg agctcacgct tgcgcaccgg
      301 ggcgtgctct ttctcgacga gcttccagag tttcgtagag acgccatcga gactttgcgc
      361 accaccatgg aagaaggcga ggtcgtgatc tcgcgagcac gtcaacgcgt gaggcttccg
      421 gccgagccca tggtgatcgc cgcgatgaac ccctgccctt gcggctacca tggcgatccg
      481 acccgccatt gccgctgcgg cgagctgcag atcgcgcgct accgcggccg ggtttcgggc
      541 cctcttctcg atcgcttcga tctgcaagtg gcggtgccgc gggtgagtgc ccgagtcatg
      601 cgcaaggcca cgcccgggga atcctcgaag gcggttcaag agagggtcga acgggcacgc
      661 gagcgcttgg gaatcacagg cgcaagacgc agcctcgaat ctctcacgag aaacgtcgcg
      721 caggaggccc tcaccctcct cgagctcggc gtcgagcgtc tgaagatgag cgctcgtgcc
      781 tacctcaaag cgcttcgcgt cgcgcacacc atcgccgtcc tcgacgaatc gagcgaagtc
      841 ctgcccgctc acacggccga aacccttcaa taccgcgttc tcgatcgcga gccgtcctga
      901 agctcgccga agagcgccat caaacatccc aaacgaaaga ggagcaagaa cacatgaagc
      961 aagcactcaa ggtcatcgac gatctcctac agaacgtcga gaaacaattc ggcaagggcg
     1021 cgatgatgcg gctcggcgac cacaaggccg aacgcgtgcc ctccattccc acgggcagcc
     1081 cctcgctcga cgaagcgctt ggctgcggcg gctacccaaa ggggcgtgtc gtcgaggttt
     1141 tcgggcctga atcgagcgga aagacgacgc tcacgctcca cgcgatcgcg cagtgccagg
     1201 ccatgggagg cgtggccgct ttcatcgatg ccgagcacgc actcgacatc cactacgcaa
     1261 agaacctggg cgtggacgcc aaggagctgc tggtcagcca gcccgacagc ggcgaacaag
     1321 ccctggacat cgcagagatc ctcgtcaaga gcggcgcgat cgatctcatc gtgatcgatt
     1381 cggtggcagc gctggtcccc aaggccgagc tcgagggcga catgggcgcc tcacacgtgg
     1441 gtcttcaggc ccgcctcatg agtcaggcgc ttcgcaagct ggcaggggcc gcacatcgaa
     1501 cgggcaccac gatcgtcttc atcaatcagc tccgcatgaa gatcggcgtc atgttcgggt
     1561 ctcccgaaac cacgaccggc ggccatgcgc tcaagtacta tgccaccgtg cggctcgacg
     1621 tgcgtcgcat cggcgcggtc aagcaaggcg atgtggtgct cggcaatcgc acacgcgtca
     1681 aggtcgtgaa gaacaagctc gcgccgccgt tccgaaaggc cgagttcgac atccgctacg
     1741 gcaagggcat cgacgcgatc ggcgatcttc tcgaccaagc ggtcgaggcc agcatcgtcg
     1801 agaagagcgg ctcgtactac cgcttcggcg gaaagaacat cgcccagggc cgggaaagcg
     1861 cgcgggatgc catggagaaa gacagcaagc tgctcgaggc gattcgcatt caactgcgcc
     1921 cccccgccga agacacgtcc aaggcggcct aggcgtttgt gaaccgggcg cttcctgtta
     1981 caagacggac gtgcgctgtc tggttacagg ggcgaccggt ttcatcgggg gcggggttct
     2041 gcgagggctc gaggcagacg gccaccaggt ggtcgcctat gttcgcgagg gctccgattc
     2101 acgcgcgctt cacgacgtcg agcgcgtggt cggtgatctc gacgatccaa gcgctctcgc
     2161 ccgggcagcc tcgggttgcg acgccttcgt ccacgccgcg ggcatcgccg atcctgctgc
     2221 gagcgtcgag gccctcggtt gggcgcacgt tgccggcacg gagaatgcgc tcaacgcggc
     2281 aaagcacgcg ggttgccggc gcttcgtgca catctcctgc gccgacgtca cgctccatga
     2341 cggaccgcgc tctttctgga acgaagaagc acagcccgcg cgcccctacg gcgagctcgc
     2401 gcgatccaag ctgcaggccg aagagctcgt acgcgtctcc ggaagcaaag ggttttggac
     2461 cattgcgcta cgacccgccc tcgtatgggg gccgggggat acgacacacc ttcccggctg
     2521 gaaggccgaa gctgagagcg gcggcatccg catcgtcgga ggaggcaaga agctcttggc
     2581 caccacgtac atccgcaacc tcgctcatgc cgtgctctgc gcccttcgaa ccggcgtcaa
     2641 caccggaaac gtctactatg tcgtcgatac ggagctctcc gtctctcgcg agctcttcac
     2701 cgagctcagc gctgcgctcg cctggccgcc gccgaagagc gccggtccat accgctgggc
     2761 ctggttctgg tcgcgaaccg ggctatcgtc cctgcacccg gccgaggtga tccggcgcgg
     2821 gcaaaccagc gcgttcgaga tgacgaaagc aaagcaagac ctcgaatacg agcccatcgt
     2881 gacgcgggag caaggcctgg ccgagctcgc ggcctggttt cgagaaaccc aggctgcgcg
     2941 atgaccgacg atccgagcaa cgagaataca gacgagcgct ggatgcggtc ggccatcgcc
     3001 gaagcccggc tcgccgcggc agagggcgaa gtacccgtcg gcgcagtcat cgtgcacgag
     3061 ggcaggatca tcgcgtgcgc acacaacgaa cgagagtcct ctcaggatcc cacccggcac
     3121 gcagaaatga tcgcaattca agaggccgca aagaagctcg gaagctggcg gctcatcgac
     3181 acgacgctct acgtcacgct cgaaccctgc cccatgtgcg ccggcgcgct cgtcaacgcg
     3241 cgtgtcccgc gagtcgtctg gggctgcgac gacccgaaag cgggcgcgac ccgaacgctc
     3301 tacgagatcg gcaccgatgc tcggctcaac catcgcttcg agtgcgttcc tggagttctc
     3361 gatgaagcgt gcagtgcact tctgaccgag tttttcggcg caattcgggc caagagcaag
     3421 ccgcgcgaca gtcgttgaca ccccaacgcg ttttgctagc ttccgccgcg gagagctgac
     3481 cgagcggtcg aaggtgcttg attcgaaatc aagtgtactg gcaacggtac caagggttcg
     3541 aatcccttgc tctccgccaa gttctttgac ctacaagttg agggaagcaa aggtcgctgc
     3601 cgaaagcagc ggccgacgcg tttaggaagg cggactccgc ctggagaggt gaccgagtgg
     3661 tcgaaggtgc acgactggaa atcgtgtgta ccgaaaggta ccgggggttc gaatcccttc
     3721 ctctccgcct tttggtcctg ccaacgtcgg cccgtgaaac ccgccaggcc cggaagggag
     3781 caacggtagc ggtaaccgac gtgtggcggg gccactttta acgctatgct gcttcgagcg
     3841 gggcaagcgg tttcactcag caatgtagcg aatcatgcgt ttttcgatcg ccttcatctc
     3901 gctcgccatg gtcattcggc ccgctctcgc gctcggtcag cagacggggg ctcaaccgaa
     3961 ggacgagcct tcttcgtcca tcgtggtgat cgacacgccg cctccgcaac cttcgccggc
     4021 gcagccgcag ccaccgtcga cgggcgtgat cctccagcgc cctggcaccg tgctcctttc
     4081 cggcaccccc gtgcgcctga agttccgctc ccccacggac ggcgtctcgt tcctccttca
     4141 aagcgccgga agctacactt cgatcagcgg cgtgaccgtc ggcatgggct ggggcggata
     4201 tggctggggc ggatggggct ggggcggata cggtttcggt ggcatggcgc cctactacgg
     4261 cgagatggtc acccaagcgt accagccgat ctgcgcagcc ccctgtgacg ccaccctgct
     4321 gtctggccgt caccgcttcg cactctcctt gaacgacgga aggccggtca acgtccccaa
     4381 ggcgctcgac ctcagcgtag attcggtggt cgaaggccgc tacgtagaca agagccgcat
     4441 gcgaaaagcc ggctgggcaa ccttcgtcgc cggcgcggta accggcatgg ccctgatgtt
     4501 cgcatcgatc aactaccacg ctgacccgtt ctacggcgat caaatccgct accgccccat
     4561 gatgttcacc ggcgtcggcc ttttcgtagg ctccatcatc accggctcgg tgctcgcctc
     4621 gcagaacgac gaagcccagg tgattgtgta cccagaagat tgaccggaca ctggcacgga
     4681 ctctggcact ggcgctggca cggacgctgg cactgacact ggcactggcg ctgacgctga
     4741 cactggcact gacactgacg ctgccgctga cactgacgct gccgctgccg ctgccgctgc
     4801 cgctgccgta cggaggggga cggctcggga tgggcagcgc agcgattggg tcgagcctag
     4861 ccttaaggcg tcttagagaa ctgggggcga gtgggtccca atcgcgagca cgcccgtcgc
     4921 gagccgtctc ccggtgacac tggcgccggc aagagtcgga ggcgccgctt agatcgcgtc
     4981 ctcgaacacg aggtcgatct tgacgaaggt ggcgtcgtcg tagtcgaggc cctggaagtt
     5041 caccgcaacg ttcttgtcgt cgaactgaat gtcgaggtcc tgagagtcga tggttgattg
     5101 ggtcgcatcc aggatcgctt cgaggatccg cggcaggcgc gagtgctccg tcaccgatac
     5161 gaggtagccc tcgaattcgg aggcggaaat cttgccgccc ttcgtcacgt tggagaagtc
     5221 gaagatgatg ctctcgccat cgatgaagac ttcgatttgg gtgtccgata cgccctcgca
     5281 gcgggcctcg gggtcgttgc tttccaggac ttcacagccg gtgagctcga cgagatccac
     5341 ctcggtgccg cccatgcgct ccacgatgcg cctcgacaag gaagagctga gctcgccacc
     5401 gcttttttgg aacagcccat cgccgcagcc cgtggtcagg gcaaacagca aggcgatggc
     5461 gaccaacgaa gcgttgcgaa atgtgttgct gagcgagtgt tccattcttc tttcccttgc
     5521 ctgagtgccc gttctgctgg gcctacggcg gggaaggaga aatggattaa ccagaggctc
     5581 gattctctat cgttcagatc gactgagcag acgcttcgaa aaaaagatca tgagccctta
     5641 atccaaatgc gagtcctcga cgtaggggtg tttgaaggcg gatttgagcc gccaaaaccc
     5701 ccaaacgcga ccgaatgagg agaacagacc atgaccaagt acctactcaa cgcagcttgc
     5761 cttagcctcc tttccttgat gctcgttgcc tgcggcgacg caggcgtgaa ccagggagac
     5821 gaagacctca acttcgacga tccgaccgtg gacgaattcg tcgacgaaga ggtcgttgat
     5881 gaagagatcg tggacgacga gctcgtggac gaagaagtcg tcgaagagct gattgctccg
     5941 ttcgacaatg acagcgcggc caacccggcg gtcgacgagt tcctgagcat cacgaacact
     6001 cgcgagatcg tctataccga ccaaatctcg gcagaagatg gcgacaacga ggacttcgtg
     6061 cagttcgagc tccccaacaa cagcaacccc aaccagagaa tcgagatcga aatcgactgc
     6121 gatgtgttcg gggatgaaaa cgtgttcgct cgcgtcgagc ttctcaacgt cgacggcaac
     6181 gatacgaccc gaatcaacgg cggcccgatc gactgcaatg aaggcccgtt ccaagtcacg
     6241 gtgcgcaatg accaggtcca gctggctcgc atctacgtcc agggagttcc tcaggagcag
     6301 accctgatcg agtacaccct cgtcgtggcc cctttctagc cgctgaccag acgcttcgga
     6361 acggtcctct gacccctgtc tcgcaggccg gctctcttcg gagggccggc cttttcgtgc
     6421 gtgcgatcag agcttcgaga accaaccgcg cagcctgccg agaagaccgc ccttttcttt
     6481 ggcgatcttc ttctggatct gcccgagggt cttgggcgat gtctcgagcg gctcgaagct
     6541 cgaagcgagc gccatgatcg aatcttggaa gtccgcgacg accgcggcga gagcaggatc
     6601 tgccgcaagc agcgcctcga gctccttttc gtcttcttcc gatgctgccc cggccgcgtg
     6661 gagagcggcg aggagctcgg cacgttcctt ttcgtcactc atgcaaccat gctccgatga
     6721 atgatgggga gcatcgtgcg caacgcacgc atgcccgcga gaattcgcga ttttacggtt
     6781 ccgaccggca acttgagcgt ttcggcgatc tgatcgtggg acatcccgag aaagtacgac
     6841 agctcgagca ccgcacgctg gtcttcactc agctgcttga acgcctgcct caaggcctga
     6901 tgccaacgcg tggacgagac gagcacgtcg ggccgccccg agtcggtccc gagcgcatgc
     6961 ctgtcgctga actgcatctg cttatcgccg taccgccgcg agcgtgctcg caacgcgtcg
     7021 agcgcccgac ttcgcgcaac ggtcacgacc caggcgacca cgctacctct ggagctatcg
     7081 tactgtggcg cgcgccgcca aagctcgacg aagacctcct gcaggatctc ctcggcctcg
     7141 gagcgtgaac ctagcatctt cgtcagcagg gctaggaccg tgctggcgta gagggaatag
     7201 agagcgctca gcggcgcgcc cgaatcgccc gctgccatgg ctgcgacgaa ctctgcgggg
     7261 tcgttcgctc gtccgtcggt catcgcgttc ccgtcaagag cggctcggct tgaatcgtgg
     7321 cgaggctgcc aacatcggag tcacggtcac gccccatcat gagctctatt cccgtagcct
     7381 cccaccattg ggacgcatgc ggctgcgacc agcgttcgat gggcaagctg cgaagttcct
     7441 ccgcgatctc cgctgccgag gccggacggt cgtcgcgctt cttctccagg cactgcatga
     7501 tgatgcgctc caggccgggg tttatatcac gctgggctac ggacgaaggg gggtccggta
     7561 ttgcgctcaa atgctgcgcg cagatttcga tggcgctcgt cccttcgaat acaaaccgcc
     7621 cagtgagcaa gtagtacccg agcgcgccca gcgcgtagag atcggaccgc gcgtcgacgg
     7681 acggaggatc caggatttgc tcggggctca tgaaacgagg cgttccgatg atcgcctcgg
     7741 acaccgtgtt cgtcatgtcg accttggaat ccacgttctt caccaagccg aaatccaaca
     7801 ctttcgtgac gtcatgaaca ccgccctgtt gagtcatcat gatgttgttg ggcttgatgt
     7861 cgcggtgcac gagaccgatc gcgtgcgcct cgtgcagcgc catcgcgcat tgtcgcatga
     7921 tgtggaccac ccgaggctcc ggaagcgtgc cttcggcctc cacgatttct agtagcgtga
     7981 ccccgtcgag cagctccatc gcatagtaga aggtcccgtc ctcggtgcat ccgtagtcgt
     8041 aaatcgtgat ggtgttggga tgcctgagct tcgcggtcat ctgcacttcg cgtcgaaacc
     8101 gatcgatgtc ctccggcgtg tttcgatcgg cgctcaagag cttgatcgcc gtgggtcgct
     8161 tcaggagccc atgccgcgcg cgatagacga cccccatacc gccctcgccg agtttcgctt
     8221 cgacgatgta ttggccgagc ttcctggcgt cgcgtgcgtc cttgcgtaga ccgtagatca
     8281 cacgcgtggc ccaggccgcg agcaaggtgg tgagtgccca cgcgaacccg atctgaatgg
     8341 cctggcttct cgctagcgtc tgcttcttct cgatcgtgcg aaagaacccg tggctgttcc
     8401 agacatccag atccaccgtg aggaagttca ggtagacggc gatgacgagg ggaattccaa
     8461 tggcgagccc aagccaagcc gtatgcttcg cggtgctggg aacgaagatc gcgcgagcga
     8521 tgagcccgaa cgatagcgcg taggcagtag tcgtggcggc accggccgcc gccgggaggt
     8581 tggcccccat cacgacgaga gcgatgctcg tcagcaccaa gccgacacct tcgacgatct
     8641 gaatggttcg cgtctgcaag ggacgcgatc gacagatcag ccagatgcat gcgaccggta
     8701 gccaagcgcc cgcgtgagtc caaaatgagg ggtgcgcgac ctccttctgc caatagccga
     8761 acacgacccc aagaaagatc cggccgatca acgagaggaa cagaagaacg gccacggtgc
     8821 gcccgaacgc agccacccgc tgctgcagga actcccggct attggcgctc tgaccgactc
     8881 ccgtgtccaa aaccccttcg ttgtagatga ccggcgtcgg gttcaaaaga agaaaacgat
     8941 tcgtcagtat aacgacgtgg tttcaacgat ttacacattc cgttaatcca actgcggtcc
     9001 accggcgtag ccccgataga agggagattt cgccatgttc acagcactca ggcagcccgt
     9061 cgtcagcaac agcgttctca atgagctcgc cacctcgggc ttctttccgg accctgccct
     9121 cttcgtctac ggcgtcctgg gcgctctcat ggtggcgagc ctcttgagcg cgtacctcct
     9181 cttcggccgc tctgattcga tcccctctta gctcgctgcg cgcagtgaat tccgcgcagc
     9241 caggcgcggc agatgggaat ggccgagccc atgcgagatc tcaattgcag tgtgccctac
     9301 tcgcagacga tgactacgtc ttcgccctgt tgctcgaatg gagcttccag agggtcgggt
     9361 ctggggtctc cggcgagtag caatccgcaa ttgtcgcact cgacgttgct ccaggctggg
     9421 tcgcggccat cggggccgct tgcgagcatg ccctcgaccc aatcgacgac ggtggggacg
     9481 cctcccgtgg tgtcgctata caccttgtca ttgccccaca tcgtatggcg agagcccgta
     9541 ccgaagtagt atcgatagtt cgatggaaca gtgtcgaagg ttgacatcga ctgagcaaga
     9601 gcggtgtcgc cgaacgcgca gctggcgtcc caccaactga gcgcgccaag tgggctaccg
     9661 gtcagcatga cgttgtagaa gccggtctgg ccgccgctgc ccccgtcgtg cttcgtcgag
     9721 tagtgcgccc agttcgtgtt tgggaactcg gtggcgactg cctcggtgta agctggcata
     9781 ccgttaccgg tggtgatcgc ctcgatcact ccagggatgt ctggaaggtt ggcgacgaag
     9841 ttccagtttc cgaactcgtt ctggagaaag tcgggcgtga tgacgccatt gccggcgtcc
     9901 gcgagcacgt gcatctgcga tgcgggccag acttcgtgaa gcagaggagc gttgaaccaa
     9961 gcgccgtatg ccccggcgct cgagccggtc acgaagacct cctcggggtt gaggaagtgc
    10021 tcgcgtgccc atttttcggc caccttggag ttgtgatagc ccttgtgttc aaccgtgacg
    10081 tcgccgtaat cctgcgttgc gtcgccgaaa tggacgtcgc agctgcaata tgaaacgaac
    10141 acgatattcc aattccgaaa cgggttgttt tcattgctca ggttggcgaa cccggtcgag
    10201 aagctgttcg gatcgtcttc tgcagtcgaa gtcttgcaga tcgcgccggt gagcggagta
    10261 ccgccgcagg tcagattgtc ccagcacgcg cctcccccct gatagtacat gagcaacttg
    10321 ttgacgctgc cgcgcttggc gaaatagcga tactcggacg cctccatgca cttcggcgtg
    10381 tacgttcgtc ccaggtattc ggtctcccct ggggtgagtg tcgtgtattg ctggtcgtcc
    10441 agcgacggtg ccacgatcgt atttcccacg agctcgtcgg tgaggttctg aaggtcgtta
    10501 cgaacctctt cttcggatgc gctggtgggg cagttttcgg aggttgcatc agcccaggcg
    10561 ctcgagaagg catcgagagc cgcgctcttc gccgcgtcga gggtcgaacc gtccaggtcc
    10621 gcgtagagat cgccgatgta cgcgctctcc gccgcgagca cgttctcgca ggcgtcgccc
    10681 gccgcagtga gcacggcagc gccacatgtg gcttggtcga tctgcccgag gtcgagccca
    10741 tcgttgatgg agctgacgat cgaggtgacc tgggcttcga tgccagaggc cgcgtcactc
    10801 gacgtcaagg cgaggtccgc gcagtctgcg ctagcgtctt gagcgtcgga ctcggccgcg
    10861 tcccaggcat ctcccagcgc cgtccgggcc gcttcttccg cggcgcctcg cgcgtcgtcg
    10921 tcttgatcgc cctcccaggc agaccaagcg tcgaacatgc tcttacagaa agtgcttgct
    10981 gccgcctgct tggcgccaac acatacgttg cccgcgtagc tttcgggtgt agtgtcgccg
    11041 ccggtgcctc ccgttccagc gcttccgcct gttccagcgc ttccgccggt tccaccgtca
    11101 cccccggacc cgcccgttcc gccgctcgaa ccggtatcgc tacaaccgac gcccatcacg
    11161 agcggaatcg ccaccagcgc cactagaccc aagtttgtaa gtcgcatgtc cacctccaca
    11221 actcaggttg tcgtacaagc ggctgcgcct agtcaagccc aaacccaggg cctgggagat
    11281 gggtacgcgg tggctcgcca accagttcgt ttcggagcga agctccgcct tttcggccct
    11341 cgcactggcg ctggcagcgg cgctgtcgct gccaacggcc ctgcgctgta tcgagcggcg
    11401 caatgcgagc tggcggactc aagaggactc gaac
//
LOCUS       DASRZF010000199        21415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2542607, whole genome shotgun sequence.
ACCESSION   DASRZF010000199 DASRZF010000000
VERSION     DASRZF010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 21415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21415
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2542607"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VFG22_08895"
     CDS             <1..213
                     /locus_tag="VFG22_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416399.1"
                     /translation="QLPGNDGVEVARQLRAHNPDLVVILMGEAAREPVSKERSTDVSA
                     YLPRPFRFEALRALIESLQLTPAPAE"
     gene            234..2102
                     /locus_tag="VFG22_08900"
     CDS             234..2102
                     /locus_tag="VFG22_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HET6416400.1"
                     /translation="MKRLLSCLCFVVSASLLGAAIAQSTPGFIHVDEIRPGMKGYGLS
                     VFRGTAPERFDVEVIDVLHNFRPDQDLILIRTPHPLLDRARGVAGMSGSPIYLDGRLA
                     GAYAYGWSYGVDPVVGVTPIANMLAELRRPVNLDMFPGARPLPSLPRAESTHRPPSKE
                     RLAGLPPYRGEHRVDALSTLRALQSKKAATEVPLGLHRSATPIMLGGLNDSVAQMLAK
                     ELEPLGFVAAQSSTGGGSDTGPAGFEPGGAIAVELARGDIAITGVGTVTYVGKGARTL
                     AFGHPMMDAGATGLPTATARILHVLVSELRSFKIAESGRPLGALVQDRQAAIVVDPDI
                     QAARIPVRVKINGIEGAPRTEWNVEVASHRMLTPSIVFGVIANAVKSTAGDVTDVIFR
                     ARSKVGIEGHGVVSLEEQGFSPMGAASPAVFSQLRMFALMEAAFANPFESSRPTSVDL
                     ELDLEASREVFQISEASVAYDEVDPGEETTIYVRLKRVDQPDTLRAVKVHIPEAAAGR
                     TVRVTVAAGNQIAIEQPIPGSFEDLIEQTQRRYPATSLVVALEMPTRGLRFRGHVVDS
                     LPESALNSLQLVSSSEDSRPFITQSRTEIELQQVVIGGARLTLRVREVPQDQLLGN"
     gene            2110..4137
                     /locus_tag="VFG22_08905"
     CDS             2110..4137
                     /locus_tag="VFG22_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416401.1"
                     /translation="MTIRKTTLQIALAGLLLATSATVAAVTTESFVLDSADAFFEGEL
                     EGAAVHSDGSVRAGAAVRRTELENVPLAYSMAERGGTTFIGTGTNGVVYKFDGKSAKP
                     FAETGELLVASLAFGADGALYAGTLPNGRIYRIDPKSGATKRFSVPEGAKHIWALHYD
                     KKRGRLIAGTGPEGKVFAIDPIGRAQEIHKAEASHIMALAGDGKGTIFAGTSDSALVL
                     AISLKNEVSVVHDFPGNEVTALDFYEGQLAVAANEFKTGPGTQFKPTPAATTPSVAPP
                     ATRPRPGTGEVWRVGADGRSEMLVGRKDTHFTSVQWGTDGAIYAGGGHEGRILRIEPD
                     ASYSIWVDVEERQILALDLRGKNPSFVTGDAAAIYRLESGKRQDAVWTSKPLDARFNS
                     SWGRLTWRGQGRVVFQTRSGNTQSPGETWSPWSKDLKQPSRIASPPGRFIQVRAKLPK
                     DDDAVVRAVELFYLPQNQRARVSNVQGKRPPLKRGETPSQPLPPTTMLTLAWQVDNPD
                     RDTLRYRVTYREEGQPTWREMFGEDTVLSEVNYVWDTDSIPDGYYVVRVEASDEGSNP
                     EALTLRSSAISEPIAIDNHPPRIEELKVRKGRVVGRVTDTLGPIARIQLSVDAGIWRD
                     LYPVDSLLDSPSERFDVQLSELSDGSHIIAIRAFDASGNQANREIIVKKGK"
     gene            4325..4975
                     /locus_tag="VFG22_08910"
     CDS             4325..4975
                     /locus_tag="VFG22_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416402.1"
                     /translation="MNISKLALIALLGGALMAFGCSDDASGDGGTGGGTGGTGGGTGG
                     TGGVQVPAGAYAFSCTLQTIPIPLPVTITINAEDPGFTEGEPSDLVTLLNFTVAPDVI
                     GLLPGLAPGAMVADISATISGVGGTPTAIEHTAEGLPFAPVADFDSDEVTTAVTPDAA
                     ATEIELSVTAMSALIDGLGDLVPGGEITLEAGAGDCGPVEPVEGSGPVTFSVTAAM"
     gene            complement(5136..6188)
                     /locus_tag="VFG22_08915"
     CDS             complement(5136..6188)
                     /locus_tag="VFG22_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04552 family protein"
                     /protein_id="HET6416403.1"
                     /translation="MSFPEFRSLSDFTLADLEGIRVLLRGGSVVDWHRLNFASDEEAE
                     AFVRAQEIDPDDEGDAARVTEVKEAAIDYLRRHYDFPVPQPVAKKDLVGLLMLASSKG
                     HRQICACAILKVMHIIHHLEARELLFVLRTADEEVFQWVEQKVYRVMAAALANDFPIL
                     EFIGGRKHKDSLYTKLLSKPDVSASQIYDKVRFRIVTRTDRDIFSVLNYVQRSIVPFN
                     FVIPGQSTNTLVRFHEYCRSEPALMKLVPSLQLPLDLEDELSPIDNRFTAPSYRIVHF
                     VADVPVRVPEKVLAAAPSAAASHGHVIFVQTEFQVIDRSTDESNEQGDASHQAYKERQ
                     KLAVMRRLKVGRFSKP"
     gene            complement(6191..6340)
                     /locus_tag="VFG22_08920"
     CDS             complement(6191..6340)
                     /locus_tag="VFG22_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416404.1"
                     /translation="MLGRRSSWGAGLLGLTAIGGCHGGIAFNALVLLVSVGIFVGTIL
                     FERSP"
     gene            6441..8804
                     /gene="lon"
                     /locus_tag="VFG22_08925"
     CDS             6441..8804
                     /gene="lon"
                     /locus_tag="VFG22_08925"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HET6416405.1"
                     /translation="MTDSIFPAGAEPILPILPLRNSVLFPATVVPVNVGRPRSVRLIE
                     EACGRERPAIGVVSQLKPEVEDPTFDQVYHTGTIARVLKVIRLNSGQYSVVLQGVSRM
                     RIEEPLGRHPTMRARVHRYHEVPAVDVEIEALAAHLRESARQLLHELPTASRESLHIL
                     DNVREPGALADLIESNLPLPNDAKQKVLETLDIRERVRLVLAQVNRQNEVLRVKEEIS
                     TMVEEEMSSSQREYLLRQQVKAIRRELGETDVDEDEIENLRERIALGRLPKEPAAAAK
                     RELRRMGNMNTASSEYQVARTYVEWLADLPWKKKTPDRLDVAQARQVLDEDHYGLEKP
                     KRRIVEYIAIRKLRRDGRAPILCFVGPPGVGKTSLGRSIARASGRAFVRVSLGGVSDE
                     AEVRGHRRTYVGAFPGRIISGLKKAGTRNPVMVLDEIDKLGRDQRGDPSSALLEVLDP
                     EQNHQFTDHYIDAPFDLSKVMFIATANQKSTIPSALLDRMEVIELPGYTPREKRSIAR
                     DFLIPRQLSEHGLSPERLDFSDEAIDMLIESYTHEAGVRKLEQQAAAVCRAIAVRLAA
                     GEDLSIMADPPFIESVLGAAKTRRQETERSARAGVATGLAWTPAGGDLLFVEASQMPG
                     TGKLHLTGNMGDVLKESAAAAFTFVRARAGDLGLSDDFVSHTDVHVHLPGGGVPKDGA
                     AAGIPLFVALASALTRVKIRPDVAMSGEITLRGNILQVGGIKEKCLAAHRAGVTRILL
                     PKRNEPDLEEVPDEVREDLEICLVSRVDEVMSLVTAIEPTGAPAAAE"
     gene            8808..11096
                     /locus_tag="VFG22_08930"
     CDS             8808..11096
                     /locus_tag="VFG22_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416406.1"
                     /translation="MFRLITFRSSATFAAVLGIVLAFIPLLAVHGVESALVLGLLLPP
                     WVAANAASYTDRNRTTRGIDLMLRSIGAGLFIWAIPMVLLGLSSLRIRQCAPGEGLAF
                     MILGPAIGTALAGCAGVWVAGYVDRPRLSPWLAASVPVSAALLGLWAFYSTPTVYVFG
                     AFAGYFPGAIYDDLVQIPTRYLTYRATIVLALLALALLFDALWDPAAGMLEFRQRGRR
                     QLGTVLVALAALGIVVASYWHGDALGHWVSEEYLTERLGKTEQGRHCIVHMPRETSPE
                     DANRSLEDCDFHVARTRALIGASSNEPVTAYFFRNPDEKKDLIGVGRTLIAKPWRREV
                     YLQMSGWPHPVLGHEIVHAVLAEAGRGPFRVASMFGGLIPNPGIVEGAAVALARDVRD
                     ELDPDQWSKILMDRNELPAARTIMSLQFSALPARRAYMAAGSIIRLLIATRGMDAFLD
                     AYRHGRIEDLDALDAEWRAYLEDVPVTAHERGVAEVELAMPSIFSSVCPHELAKLRAH
                     LGGDTAARDDLRTIETCRAILDINESEAEAQAALVGALARSGQNAEALATLEALRDAM
                     AAPKPIVAAALEAYADASWSLGRLDEAAKLYDELLAIPRTDGATRQAEVKKLALGARD
                     DERSLIYQMLLDSSPSAVVVHLAHALAAIRDDGLGQYLEARQLMGQSRYALALPLLQD
                     AERLGLPSVRLERELSRMLGITYFALGRYDESAEAWREQAWTSRAARAEAERWIERIE
                     YAQTGTLSAVLPSPSSAPRAAP"
     gene            complement(11047..11913)
                     /locus_tag="VFG22_08935"
     CDS             complement(11047..11913)
                     /locus_tag="VFG22_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416407.1"
                     /translation="MEPTELTPAEFDGARKNRYRPRLPWARIGFVVLILALISGAYFW
                     RQKVRADALRERIEALHAERVAPILAQLEATRVDLEAKALDAKSGAANRLVEAEVPLS
                     ALHEKKVVYLKLRAPELASEETLRAAIDSEEVDAIGACLGLELTPLLELSDVPEVLTV
                     KWLRRVDDTNDMQRLSVREEQLRRAIERELPALAQRVPADYFLLLVVQGKSRLRDPVD
                     VFLWDLAQDKLVLRSRTENRGRLITVRSQIGPKSDSAKAKGDPIVIADCSIAAHIKAQ
                     LGEPTMDLAAPR"
     gene            complement(11938..12471)
                     /locus_tag="VFG22_08940"
     CDS             complement(11938..12471)
                     /locus_tag="VFG22_08940"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET6416408.1"
                     /translation="MAIKRPEPELLRHIPLKFVRERVSGHARKSVDHQIPLIPFIDFL
                     IVLVIFLLMSFSASGELVAQQPTITMPDAKNTAKIEISPVIAVDERVITLDGTRVADT
                     QTQGQSAQVDRIEPLIQGLETEKRKWETIHPSEPFAGQVIVQADRNIDFRVVKKVMFS
                     AAAAGYGNVSFAVNQRE"
     gene            complement(12481..12972)
                     /locus_tag="VFG22_08945"
     CDS             complement(12481..12972)
                     /locus_tag="VFG22_08945"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET6416409.1"
                     /translation="MAAIDSGGGNSGKKSVNQEIPLIPFIDLLLCCVMFLLATAVWNQ
                     LAAVNANQQVPGQASTEDAPPEDEKVKLILQVRNSGYVLGSSAGESIDISKRAVADQK
                     EFDVDELQTKLAQWKQAWDKRDDLIVAPEDGVRYEDVIVAMDVAAAAGFTTLSMADGA
                     AFL"
     gene            complement(12981..13631)
                     /locus_tag="VFG22_08950"
     CDS             complement(12981..13631)
                     /locus_tag="VFG22_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET6416410.1"
                     /translation="MAKLAYHFEAGGWAMWLILTCLVFAIGFTIERAMYLYKASIDKD
                     EFIAKMQKCITAGDVAGAIKVCSAEHNPLARIVKQGLLKVNRPDAEVQAAMDEAALKE
                     LPLIERRTPYLGLLANVGMLCGLFGTVVGLITAFGEVAKADAASKATGLAKGIEEAMN
                     CTAFGLITAVFALLMMGLLNGKTQKIIDDINAATVQVLNLVVGNRSKVDLQNLPAE"
     gene            14062..14694
                     /locus_tag="VFG22_08955"
     CDS             14062..14694
                     /locus_tag="VFG22_08955"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET6416411.1"
                     /translation="MHEIWEALLEGAPFSFINLAVLAFGVGVIIDRAWYIRMNYSVDA
                     AEFMNRIRKLVQAGNIDRAIRQCEAKAVPLLEVVKSGLTQVNRGEEAVIASMEEKMSE
                     VIPALEKRTGSLWTLANIATLIGLLGTISGLIRAFKAVGTIDDPSQKTALLSAGIAEA
                     MWNTFLGLLIAVIFMVAHLVLHGLTKRHKHEMEKVTMQLENLLTLRRQGG"
     gene            14707..15264
                     /locus_tag="VFG22_08960"
     CDS             14707..15264
                     /locus_tag="VFG22_08960"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET6416412.1"
                     /translation="MPARLTPRQRAYVRKRTIVPDADPSELSDELNIVPFLDIVVNII
                     MFLLMTLTTVAFFSQVEAALPEYRKGGIGKRAAADEALNLNVTVTREGVVVSGSGGKL
                     ARGCTTTAPGKVITVPAGMNGEHDWKALTTCVTRVKEQFEDETRVTLSADPEVPYEDL
                     IAAMDAVRGSESELFPDVLLSAGIR"
     gene            15264..15866
                     /locus_tag="VFG22_08965"
     CDS             15264..15866
                     /locus_tag="VFG22_08965"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET6416413.1"
                     /translation="MANESSRPPHDSLAPPVSEKPVSMSYVNRVVRAKLRRRAEPEHF
                     GLNIYPMMDMLTILLVYMMAIFATSSAAAVQESSELRIPYSTSKVEVSEAIGVQISRS
                     GVAVDGKPVLELRNGIVDPSLKQGGSSGFLIMPLYKTLSEIRDRKKRTAARFANQPFV
                     GDVQIVADKRTPYRTLSEVIYTLGQTEFKNLRFVVNKKSE"
     gene            complement(16097..17380)
                     /locus_tag="VFG22_08970"
     CDS             complement(16097..17380)
                     /locus_tag="VFG22_08970"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HET6416414.1"
                     /translation="MATKVTMPQLGESVVEGTIGKWLVSVGQSVAKDEPLVEILTDKA
                     DSELPSPVAGVVTQLLASADDIVAVGDAICEIDESGKAEVTPPTKTKQAPKAAVSDAP
                     SPTSPSVRKLAREKDVDLSSVRGSGDGGRIVKDDVMAAIGSSTAPERMATPPPPPPEP
                     AARPSAPPAPQRPAPAPSRGTGPSGAFRLPPYVPKEGDELVPFTRRRRIIADHMVYSK
                     QTAPDVVTFAECDLHRTAVLREQHKAHFKKDGVNLTYLAFAIAATARALREFPEINSR
                     VLDDAFVKLAEVNIGIAVDTDEGLVVPVIRNTDDLTIRGVARAVADVAERARTGNLTP
                     NDLSGKTFAISNPGRRGNLVGGAIISQPNVAILRLGTIKKRVVVIEHEGEDLMAIRPV
                     MHMALTYDHRVVDGVHANGFLYRIAEILEYGDFEL"
     gene            17518..17844
                     /locus_tag="VFG22_08975"
     CDS             17518..17844
                     /locus_tag="VFG22_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HET6416415.1"
                     /translation="MLDALNREDRLQLMRFVCSFAWADLEVSDEEREFIVKMVIRLGL
                     NEEEQDQVAQWLEVPPRADDLDPADIPREHRKLFLDAAKAMILSDGTVEDAEAENLII
                     LDQLLR"
     gene            complement(17852..19003)
                     /locus_tag="VFG22_08980"
     CDS             complement(17852..19003)
                     /locus_tag="VFG22_08980"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HET6416416.1"
                     /translation="MSLRRKKTIGIDLGTTNTVIARNFESLSIEGGSCVMPSAVAFPP
                     SGVTLIGQAARRRRAMDPRNTIISAKRIIGARWRSSYASQFRHHYPYDLAETADGGVA
                     FRTRLGLFSPVEIGSLVVGGLCRLTKCPVEDVHAVVTAPVGFDGRRRDATALAVSQAR
                     FASVRVIEEPIATALAYLNRSSLKHAFVYDLGGGTFDAAVVDCSSYPFRVIGHGGDPY
                     LGGDDVDRTIALHIADRTLARDGWDLSNDAVIFDRLVLACELAKCELSESEQAAIAIP
                     SVDPAAPESCGVISITREEVQQLTLDLVRRTFVICDEVLAQAGVKARDMDAVFLAGGS
                     TLLPGLRRYIESYFGKRPRFDLDPMQVVSLGASLAAARPAVSELLDPCA"
     gene            19182..20237
                     /locus_tag="VFG22_08985"
     CDS             19182..20237
                     /locus_tag="VFG22_08985"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HET6416417.1"
                     /translation="MPADFRKLQIAGIIQETHDTRSYVLAVPESLREEFRYRAGQFLT
                     FEVPWDGMQLRRCYSLSSAPETDPWPKVTVKRVDEGRVSNWFNDRLQVGDSILVQPPE
                     GRFVLRSNEGDHGIVLFGGGSGITPVLSILKSALRKTSRKVKLIYANRDPRSIIFRDE
                     IALWLAEFPDRLEVVHHLDSEKGFMSIADVQAQIRGWENAEFFVCGPTGYMDTVEEAF
                     KASQIDTGQTKFERFISPVDPDRRDASAAPVPEQSADEIPDSFKMQLEGHSLEVPYEK
                     GLTLLESAVKAGHKPPSSCEDGYCGCCMALLKSGSVKMSNHDALEPSDIERGWVLACQ
                     ARPASREPIEVDFDAEY"
     gene            complement(20234..20764)
                     /locus_tag="VFG22_08990"
     CDS             complement(20234..20764)
                     /locus_tag="VFG22_08990"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET6416418.1"
                     /translation="MPSYPLEEVEEAFRHFWQTGMIREDWNAWADLFTEDVVYYERVL
                     GTMRGRETVRAWIVPLMEEYGEIYGVYDWHMADPSGRVVFHMINRRDHPSGEGAMDFP
                     GITVLQYAGNNQWSSEEDYWAEKLGIQQWQAYGAALKTYGPEHRKNRTRKDWGSGPDW
                     TIGKSSFWDWPDRQRP"
     gene            complement(20764..>21415)
                     /locus_tag="VFG22_08995"
     CDS             complement(20764..>21415)
                     /locus_tag="VFG22_08995"
                     /inference="COORDINATES: protein motif:HMM:NF039286.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416419.1"
                     /translation="ANMVMCWHHAEGEPPTFEIPDEIPEWGTKDGVRNEEWTEFVDRE
                     WTIRSRNQEMAENAVDSAHFHYLHGTTNMPKSEATIDGPILRVFSGAGMETPRGTVDG
                     SIESLNYGFGLAVVRFKGIVETLNVASTTPIDADHVHLRFHFCVKKTAGAEMARGVGK
                     AFVAEVTRQLEQDIPIWENKIQYEKPLLCDGDGPIGIFRRWCTQFYCWPEGKEAAE"
BASE COUNT         4010 a         6843 c         6690 g         3872 t
ORIGIN      
        1 cagttgcccg gaaacgacgg agtcgaagtt gcgcgacagc tccgggcgca caatcccgac
       61 ctcgtcgtca ttctcatggg tgaagcggcc cgagagccgg tctccaagga gcggtccacc
      121 gacgtgagcg cttacctgcc caggcctttt cgattcgagg cgctgcgagc gctgatcgag
      181 tcgctccaac ttacaccggc ccccgcggag taggcctgct aggcttcttc agcgtgaagc
      241 ggctgctctc ctgcttgtgt ttcgtagtga gcgcgtcgct cttgggcgcc gccattgccc
      301 agtcgacacc gggcttcatt cacgtcgacg agattcgccc aggaatgaag ggctatggtc
      361 tctcggtatt tcgcggcacg gctcccgagc gcttcgacgt cgaagtcatc gatgtcctcc
      421 acaacttccg ccctgatcag gacttgatcc tgatccgcac gccccatccc ctgctcgacc
      481 gggcgcgggg cgtcgcaggc atgagcggaa gcccgatcta cctcgatgga cgcctcgctg
      541 gcgcctacgc ctacggctgg agctacggag tggacccggt ggtcggcgtc acgcccatcg
      601 cgaacatgct cgcggagctc cggcgacccg tgaacctcga catgtttcct ggcgcgaggc
      661 cgcttccttc gctgccacgc gccgaatcga cccaccgacc gccctcgaag gagcgtctcg
      721 ctggcttgcc cccgtaccgg ggcgagcatc gagtcgacgc gctgtcgacg cttcgcgcac
      781 tgcagagcaa gaaggccgct acggaagtgc ccttggggct tcatcgcagc gccaccccga
      841 tcatgcttgg cggtttgaac gactccgtcg cccagatgct cgcaaaggag ctcgagccct
      901 tgggcttcgt ggccgcgcaa tcgagcacgg gcggaggctc ggacaccggg ccagcgggct
      961 tcgagccggg cggcgcaatc gcggtcgagc tcgcgcgcgg cgacatcgcc atcacggggg
     1021 tcggcaccgt cacgtacgtg ggcaagggag ctcgaacact ggcgtttggt cacccgatga
     1081 tggacgcggg cgccacgggg cttccgacgg cgaccgcgcg catccttcac gtgctggtga
     1141 gcgagctccg ctcgttcaag atcgccgagt cgggccgccc tctgggtgcc ctcgtgcagg
     1201 accgacaggc ggcgatcgta gtcgaccccg acattcaagc cgcgcgcatt ccggttcgtg
     1261 tcaagatcaa tggcatcgag ggtgcgccga ggacggaatg gaacgtcgag gtagcgagtc
     1321 accgcatgtt gacgccttcg atcgtcttcg gagtgatcgc gaacgcggtc aagtcgacgg
     1381 ccggcgatgt caccgatgtc atcttccggg ccaggagcaa ggtcggcatc gaaggtcacg
     1441 gcgtagtctc gctcgaagag cagggattct ccccgatggg ggcagcgagc cctgccgtgt
     1501 tctcgcagct gcgcatgttc gcgctgatgg aagccgcttt cgccaacccg ttcgagagca
     1561 gccgcccgac ctccgtcgac ctcgagctcg acctggaagc gagccgtgaa gtgtttcaaa
     1621 tctccgaggc ttcggtcgcc tacgacgaag tggaccctgg cgaggagacc acgatttacg
     1681 tccggctcaa gcgcgtggac cagcctgata cgctccgggc ggtgaaggtc catataccgg
     1741 aggccgccgc cggacggacg gttcgcgtga cggtggccgc gggcaatcag atcgcgatcg
     1801 agcagccgat tccgggctcg ttcgaggacc tgatcgagca aacccagcgg cgttatcccg
     1861 caacgtccct cgtagtggcg ctcgagatgc ccacccgggg cctgcgattt cgcggccacg
     1921 tggtcgattc gctcccagag tccgcactga attcacttca gctcgtcagt agctcggagg
     1981 acagcaggcc tttcatcacg cagtcccgaa ctgagatcga gctgcagcag gtggtgatcg
     2041 gtggcgcgcg cctcacgctt cgcgtccgcg aggtcccgca ggaccagctt ctcggcaact
     2101 aggaaaacga tgaccattcg aaaaacgacc ctccaaatcg ccctcgccgg cctcctgctc
     2161 gcgacgagcg cgaccgtggc cgcagtaacg acagaaagct tcgtgctcga cagcgcggac
     2221 gctttcttcg aaggcgagct cgagggcgcc gcggtccact ccgacggttc cgtgcgtgcc
     2281 ggcgccgcgg ttcggcgtac ggagctcgaa aacgtgcctc tcgcctacag catggcggag
     2341 cgcggaggaa cgacattcat cggaaccggc accaacggcg tcgtctacaa gttcgacgga
     2401 aagagcgcga agccgttcgc agaaaccggc gagctcctcg tcgcatcgct ggcattcggg
     2461 gcggacggtg cgctctatgc ggggacgctc ccgaacggcc gcatctaccg gatcgatccc
     2521 aagtccggag cgacgaagcg cttttcagtc cccgaaggcg caaagcacat ctgggcgctg
     2581 cactacgaca aaaagcgcgg acgcttgatc gctgggacag gaccggaagg caaggtgttc
     2641 gccatcgacc cgatcgggcg ggctcaggaa atccacaagg ccgaggcctc ccacatcatg
     2701 gcgctcgccg gcgacggaaa agggacgatc ttcgctggta cgagcgattc ggccctggtg
     2761 ctcgcgatct ctctcaagaa cgaggtctcg gtcgtgcacg acttcccggg gaacgaggtg
     2821 accgccctcg atttctacga ggggcagctc gcggtcgccg caaacgagtt caagacaggg
     2881 ccaggaacgc agttcaagcc gactcccgca gcgacgactc cgagcgtcgc cccgcccgcg
     2941 acccgtcccc gccccggaac cggagaggtc tggcgcgtcg gggccgatgg gcgctccgaa
     3001 atgctcgtcg gtcgcaagga cacccacttc acctcggtcc agtgggggac ggacggcgcg
     3061 atctacgcag gtggcggcca cgagggacgc atccttcgaa tcgaacccga cgcgagctac
     3121 tcgatttggg tcgacgtcga agaacgccaa atcctggcgc tcgaccttcg cgggaagaac
     3181 ccttcgttcg tcaccggcga cgccgccgcc atctatcggc tggagtccgg gaagcgccag
     3241 gacgcggtct ggaccagcaa accactcgac gcacgcttca actcgagctg gggccggctc
     3301 acatggcgag gtcaggggcg cgtagttttc caaacacgct cggggaatac gcaatcgcca
     3361 ggcgagacgt ggagcccttg gtcgaaggac ctcaagcagc cgagccggat cgccagtccg
     3421 cccggccgct tcattcaagt gcgcgcaaag ctccccaagg acgacgacgc ggtcgttcgc
     3481 gccgtggagc tcttctatct gcctcagaac cagcgcgcac gcgtctcgaa cgtgcagggc
     3541 aagcgaccgc cgctcaaacg cggagaaacg ccgagccaac cactcccgcc gacgacgatg
     3601 ctcaccctcg catggcaggt ggacaacccc gaccgggata ccttgcgcta tcgcgtcacc
     3661 taccgggaag aaggtcagcc gacttggcgc gagatgttcg gtgaagacac ggtgctcagc
     3721 gaggtcaact atgtctggga caccgacagc atccccgacg gctactacgt cgtgcgcgtc
     3781 gaggcctcgg acgaaggctc gaacccagag gccctgacac tccgctcgag cgcaatctcg
     3841 gaaccgatcg caatcgacaa tcacccgcca cggatcgaag agctcaaggt ccgcaaagga
     3901 cgcgtcgtcg gccgcgtgac cgatacgctc ggcccgatcg cgcgcattca gctctcggtg
     3961 gacgcaggga tctggcgcga tctctacccc gtggactccc tgctcgacag cccgagtgag
     4021 cgcttcgatg tccagctcag cgaattatcg gacggttctc atatcattgc gatccgggcg
     4081 ttcgatgcct cgggcaacca ggcgaaccgc gaaatcattg tgaaaaaagg caagtagcgc
     4141 gggaacaccg ccgtcgggcc gggacggttc gatttgatgg caggcggaag gccctggcat
     4201 ctgataatct gttctcttac tttgattcac ttggttggag atccggggga agtcgtggta
     4261 agcacgcgca ctcaaaaggg gcgacgtgga tccggactat cccggacctt aaggagaaga
     4321 acgaatgaac atcagcaaac tagctttaat cgcactgctt ggcggtgcac tcatggcctt
     4381 cggctgcagc gacgacgcga gcggtgacgg cggcactggc ggcggcactg gcggcactgg
     4441 cggcggcact ggcggcactg gcggagttca agtgccggct ggcgcgtacg ccttcagctg
     4501 cacactgcag accatcccca ttccgctccc agtgaccatt acgatcaacg cggaagatcc
     4561 tggtttcaca gagggtgaac cctcggatct ggtaacgttg ctcaacttca ccgttgctcc
     4621 ggacgtcatc ggtcttcttc cgggcctcgc gccgggcgcg atggtcgctg acatcagcgc
     4681 cacgatatct ggcgtgggtg gtacaccgac agcgatcgag cacaccgcag agggcctgcc
     4741 gtttgcgccc gtcgctgact tcgactcaga cgaggtcacg actgcggtca cgccggacgc
     4801 ggctgcaacc gagatcgagc tttcggtgac cgcgatgtca gccctgatcg atggccttgg
     4861 cgatctcgtc cccgggggtg agatcacgct cgaagctggc gcaggcgatt gtggcccagt
     4921 tgagcccgtg gaaggctccg gtccggttac gttttccgta accgcggcga tgtaacgatt
     4981 cctcggccca cgtaggccga aatcgtctag acacgacgaa gggcggatgc cgagaggcat
     5041 tcgccctttg tcttttctgc tgcgcctgcg aacacgcggc cgacctcgaa tcgatgcatg
     5101 gccgcgaacg gtttgtccgg gactcgaacc gcgcctcagg gcttgctgaa gcgcccgacc
     5161 ttgagcctgc gcatgacggc cagcttctgt cgctccttgt acgcctggtg gctcgcgtcg
     5221 ccctgctcgt tgctctcatc ggtcgaacgg tcgatcacct ggaactcggt ctgtacgaag
     5281 atgacgtgcc catgcgaggc cgcggcggac ggcgccgccg caagcacctt ttccggcacc
     5341 cgcaccggca cgtccgcgac gaagtgcacg atgcggtagc tcggggccgt gaagcgattg
     5401 tcgattggag aaagctcgtc ctccagatcc agcggtagct gcaggcttgg cacgagcttc
     5461 atgagcgccg gctccgagcg gcagtactcg tggaaccgaa ccaaggtatt ggtcgactgg
     5521 cccgggatga cgaaattgaa gggaacgatg ctccgctgca cgtagttgag caccgaaaag
     5581 atgtcccggt ccgtgcgggt cacgatgcga aatcgcacct tgtcgtaaat ctgcgacgcg
     5641 ctcacgtcgg gcttcgaaag aagcttggtg tagagcgagt ccttgtgctt gcggccaccg
     5701 atgaactcca gaatcggaaa gtcgttggcc agcgccgcgg ccatgacccg gtagaccttc
     5761 tgctccaccc attgaaagac ctcctcgtcc gccgtacgca aaacgaaaag aagctcgcgg
     5821 gcttcgaggt ggtgaatgat gtgcatcacc ttcagaatcg cgcaggcgca aatctgccgg
     5881 tgacccttcg aggaagccag catcagcagg cccaccaagt ctttcttggc aaccggttgc
     5941 ggcaccggga aatcgtagtg gcggcggagg tagtcgatcg cagcctcctt gacctccgtg
     6001 acgcgtgccg catcgccctc gtcatcggga tcgatctcct gggcccggac gaatgcctcg
     6061 gcctcctcgt cgctcgcgaa attgagccgg tgccagtcga ccaccgagcc tccgcgcaaa
     6121 agcacgcgga taccctcgag gtcggccagg gtgaagtcac tcagactgcg gaactccgga
     6181 aaagacacaa ttaaggggac cgttcgaaca aaatcgtgcc tacgaagatg ccgacgctga
     6241 ccagcaagac gagggcgttg aacgcaatcc cgccgtgaca gccgccaatc gcggtcagtc
     6301 ccagcagtcc agctccccaa ctcgatctcc gtcccagcat cacgggcgcc gggttgatac
     6361 cagccgaccg cgacctgcgc cagtggattg cgctggcgca ttgctggtag gctagatctc
     6421 gaatatcgtt ggcaaaacca atgaccgatt cgatctttcc tgcaggtgcc gagccgatcc
     6481 tgcccatttt gcccctgcgc aattcggtgc tcttcccggc gaccgtcgtg ccggtcaatg
     6541 tcgggcggcc tcgatcggtc cggctcatcg aagaggcctg cgggcgcgag aggcccgcga
     6601 tcggggtggt ttcgcagcta aagcccgagg tcgaagaccc gaccttcgac caggtctacc
     6661 atacggggac gatcgcccgc gtcctcaagg tcattcgcct gaactcgggg cagtacagcg
     6721 tggtgcttca gggcgtgagc cggatgcgca tcgaagagcc gctcggcagg cacccgacca
     6781 tgcgcgcgcg cgttcaccgc taccatgaag tcccggcggt agacgtggaa atcgaggcgc
     6841 tcgcggctca tctcagagag tcggcgcgcc agttgctcca cgagcttcca accgcttccc
     6901 gtgaatctct ccatattctc gacaacgttc gcgagcccgg cgcgctcgcc gatctcatcg
     6961 agtcgaatct ccccctgccg aacgacgcca agcagaaggt tctcgaaacg ctcgacattc
     7021 gcgagcgtgt ccgcttggtc ctcgcccagg tcaatcgcca aaacgaggtc cttcgggtca
     7081 aggaagagat ctcgaccatg gtcgaagaag agatgtcgag ctcgcagcgc gagtacctcc
     7141 tccgccaaca ggtgaaggcc atccgccgtg agttgggcga aaccgacgtc gatgaggatg
     7201 aaatcgagaa ccttcgtgag cgaatcgcgc ttggccggct tccgaaagag cccgcagctg
     7261 cggcaaagcg cgagcttcgc cgaatgggca acatgaacac ggcgagcagc gaataccaag
     7321 tcgcccggac ctacgtcgag tggctcgcgg acctcccttg gaagaagaag accccggatc
     7381 ggctcgacgt tgctcaagcg cgacaggtgc tcgacgagga ccactacgga ctcgaaaagc
     7441 ccaagcgccg aatcgtcgag tacatcgcca tccgcaagct tcggcgtgat ggccgcgctc
     7501 ccattctctg cttcgtcggt ccccccggcg taggaaagac ctcgctcggt cgctcgatcg
     7561 cccgtgcctc gggccgggcg ttcgtccgcg tctccctcgg cggtgtgtcc gacgaagccg
     7621 aagtgcgtgg gcatcgccgg acctacgtcg gtgcgtttcc gggacgcatc atatctgggc
     7681 tcaaaaaggc cggcacgcga aacccagtga tggtcctcga tgaaatcgac aagctcggcc
     7741 gagaccagcg cggcgatccg tcgagcgccc tactcgaggt gctcgacccc gagcagaatc
     7801 accaattcac cgaccattac atcgacgcgc cgtttgacct cagcaaggtg atgttcatcg
     7861 ccactgcgaa tcagaagagc acgatcccca gcgcactgct ggaccgcatg gaggtgatcg
     7921 agctgcctgg gtacacgccg cgcgagaagc gctccatagc gcgtgatttc ctgattccaa
     7981 gacagctcag cgagcacggg ctctcgcccg agcggctcga cttttcggac gaagcgatcg
     8041 acatgctgat cgagtcgtac acgcacgagg cgggggttcg aaagctcgag caacaagccg
     8101 ctgcagtctg cagagcaatt gcggtacggc tcgccgccgg agaagacctt tcgatcatgg
     8161 ccgacccgcc cttcatagag tccgtgctcg gggcggccaa gacgcggcgg caagagactg
     8221 agcgcagcgc gcgagcaggc gtagcgacag gccttgcctg gaccccggcc ggcggcgacc
     8281 tgctcttcgt cgaagcgtcc cagatgccgg gcacgggcaa actccacctc accggcaaca
     8341 tgggagatgt attgaaggaa tcggctgcgg ccgcgttcac cttcgtgcga gcgcgcgccg
     8401 gagacctggg gctctccgac gacttcgtga gccacaccga cgttcacgtg cacctgcccg
     8461 gaggcggcgt tccgaaagac ggcgccgctg caggtattcc cttgttcgtg gcgctcgcat
     8521 ccgcgctcac gcgggtgaag atcaggccgg acgttgcgat gagcggagaa atcacactgc
     8581 gcggaaacat cctccaggtc ggcggcatca aggagaagtg ccttgcggcg caccgtgcag
     8641 gggttacccg catcctcctg ccgaagcgaa acgagccgga cctcgaggaa gtgcccgatg
     8701 aggttcgcga ggatctcgaa atctgtctgg tgtcccgagt cgacgaggtc atgagcctcg
     8761 taaccgccat cgaaccgacc ggggcgcccg cggccgccga ataagccgtg ttccgcctca
     8821 tcacctttcg ctcgagcgca accttcgccg ctgtcctcgg catcgtactc gccttcattc
     8881 ccctgctcgc cgtgcacggg gtcgagagcg ctttggtact cggactgctg cttccaccct
     8941 gggtggccgc caacgccgca agctacacgg atcgaaaccg aacgacacgc ggcatcgatc
     9001 tgatgctccg cagcatcggc gcgggactct tcatctgggc catccccatg gtcttgctcg
     9061 ggctgagctc gcttcggatc cggcaatgcg cgccaggcga aggcctggcc ttcatgatcc
     9121 tcggtcccgc gatcggcaca gcgctcgcag gctgcgcagg cgtttgggtt gcgggctatg
     9181 tcgatcggcc gcggctctct ccctggcttg cagcctccgt gccggtcagc gcagcgctcc
     9241 tcggcctctg ggcgttttac tcgactccga cggtgtacgt gttcggagcg tttgcgggtt
     9301 acttccccgg cgcgatctac gacgacttgg tccagatccc cacccgctac ttgacctaca
     9361 gagcgacgat cgtcctcgcc ctgctcgctc tggctttgct cttcgatgcg ctctgggacc
     9421 ccgccgcggg aatgctcgag tttcgccaaa gaggacgaag gcagctcggt acggttctcg
     9481 tggcgctggc tgcgctcggc atcgtcgtcg catcgtattg gcacggcgac gcgctggggc
     9541 actgggtgag cgaggagtac ctcaccgagc gactcggcaa gaccgaacaa ggtcggcatt
     9601 gcatcgtgca catgccgcgg gagacctcgc ccgaggacgc aaatcgatca ctcgaagact
     9661 gtgatttcca tgtggcgcgc actcgtgcac tgattggcgc ttcgtcgaac gagcccgtga
     9721 cagcgtactt ctttcgcaat ccagacgaaa agaaggactt gatcggcgtg gggcggacac
     9781 tgatcgcgaa gccttggcgc cgcgaggtct atcttcagat gtctggctgg ccgcatccgg
     9841 tgctcggaca cgagatcgtg catgcggtcc tcgcagaggc gggacgcggg cctttccgag
     9901 tcgcatcgat gttcggcgga ctgataccaa atcccgggat cgtcgaaggt gcggcggttg
     9961 cgctcgcccg ggacgttcgc gacgagctcg accccgatca atggtcgaag atcttgatgg
    10021 accggaacga gctccccgct gcgcgcacga tcatgagcct tcagtttagc gccctcccgg
    10081 cgcggcgcgc gtacatggcg gccggatcga tcatccgtct cttgattgcg acacgcggaa
    10141 tggacgcgtt cctcgatgcg taccggcacg gtcgaatcga agacctcgat gcactggacg
    10201 ccgagtggcg agcctacctc gaagatgtcc cggtcacggc gcacgagcga ggcgtggccg
    10261 aggtcgagct cgcgatgcct agcatcttct cgtcggtctg cccgcatgag ctcgcgaagc
    10321 tccgggcaca cctcggcgga gacaccgcgg cccgcgatga cctgcgcacg atcgagacct
    10381 gccgggcgat cctcgacatc aacgaaagcg aagccgaggc ccaagccgcg ctcgtgggcg
    10441 cgcttgcgcg cagcgggcag aacgccgaag cgctggccac actcgaggcg ctccgggacg
    10501 ccatggcggc gccaaagccc attgtcgctg ctgcgctcga ggcgtatgcc gacgcgagct
    10561 ggtcgctcgg caggttggac gaggccgcga agctctacga tgagcttctc gccattcccc
    10621 gcaccgacgg cgcgacccgt caggccgaag tgaagaagct cgcgctcggg gcccgtgacg
    10681 acgagcgcag cctgatctac cagatgctcc tcgacagttc gccgagcgca gtggtggtcc
    10741 atcttgcaca tgcgcttgcg gcgattcggg acgacggcct cgggcagtac ctcgaggcgc
    10801 ggcagctcat ggggcagagc cgctacgccc tcgcgcttcc ccttctccaa gatgccgaac
    10861 gactcggcct gccgagcgtg cgtctcgagc gcgagctgag ccgcatgctg gggatcacgt
    10921 acttcgcgct cggtcgctac gacgagtcgg ccgaggcctg gagagaacaa gcctggacga
    10981 gtcgcgctgc acgcgccgaa gcggagcgct ggatcgagcg catcgagtac gcacagacgg
    11041 ggactctcag cgcggtgctg ccaagtccat cgtcggctcc ccgagctgcg ccttgatgtg
    11101 cgctgcgatc gagcaatcgg cgatgacgat cgggtcgccc ttggccttgg cagagtcgga
    11161 tttcgggccg atctgactac gcaccgtgat caaacgccca cggttctccg ttcgcgagcg
    11221 caggacgagc ttgtcctgag cgaggtccca gagaaacacg tcgacgggat ctcgaagccg
    11281 gcttttcccc tgcaccacga gaagaaggaa atagtccgcc ggcacccgct gcgctaaagc
    11341 cggaagctct ctctcgatcg cgcgcctcag ttgctcctcg cgcacggaga ggcgctgcat
    11401 gtcgttcgtg tcatcgactc gccggagcca cttcacggtc aagacctcgg gcacatccga
    11461 gagctcgagg agtggcgtca gctcgaggcc gagacaagca ccgatggcgt ccacttcctc
    11521 cgaatcgatc gccgctcgaa gtgtctcctc gctggcgagc tcgggcgccc tcaacttgag
    11581 gtagacgacc ttcttttcgt ggagcgccga aagcggcacc tcggcttcga ccagacggtt
    11641 cgctgcgccc gacttggcat cgagagcctt ggcctcgagg tcgacccttg tggcctctaa
    11701 ctgcgccaga atcggcgcaa cgcgctcggc gtgtagcgcc tcgatgcgtt ccctcagagc
    11761 gtccgcacgg accttctgcc gccagaagta cgccccggag atcaaggcca ggatcagaac
    11821 gacgaaaccg atacgcgccc acggaagacg tggccggtat cggttctttc gagcgccgtc
    11881 gaactcggca ggagtgagct cggtcggctc catgatgcga agcttactcg aagatcgcta
    11941 ctcgcgctga ttcacggcaa aactcacgtt gccgtagccc gcggctgctg ccgagaacat
    12001 caccttcttc acgacccgga agtcgatgtt gcggtccgct tgcacgatga cctggcccgc
    12061 gaacggctct gaaggatgaa tggtctccca ctttcgcttt tcggtttcga gcccctgaat
    12121 cagcggctcg atccggtcga cctgtgcgga ctgcccctgg gtctgcgtgt ccgcgacccg
    12181 cgtcccgtcg agcgtgatca cacgttcgtc gaccgcgatg accggcgaga tctcgatctt
    12241 cgcggtgttc ttggcatccg gcatcgtgat cgtcggctgc tgcgcgacga gctcgccgga
    12301 agcagaaaac gacatgagca ggaagatcac gagaacgatc aggaagtcga tgaaaggaat
    12361 cagcggtatc tgatggtcca cggacttgcg cgcgtgaccg gacacccgct cgcgcacgaa
    12421 cttcagcgga atgtgacgga gcagctctgg ttctggcctc ttgatcgcca tgatttcccc
    12481 ctacaggaat gcggctccgt cggccatcga caaggtcgtg aaccctgccg ctgccgcgac
    12541 atccattgcc acgatgacgt cctcgtatcg aacgccatcc tcgggcgcga cgatcaagtc
    12601 gtcacgcttg tcccaggcct gcttccactg agcgagcttg gtttgcagct cgtcgacgtc
    12661 gaactccttc tggtccgcga cggcacgctt actgatgtcg atgctctccc cggccgagga
    12721 ccccagcacg tagccggagt tccgaacctg caggatgagc ttcacctttt cgtcctccgg
    12781 gggcgcgtct tccgtggaag cctgcccggg gacctgctgg ttggcgttca cggcggcaag
    12841 ttggttccaa accgcggtgg ccagcaagaa catgacgcag cagagaagga ggtcgatgaa
    12901 cgggatcagg gggatctcct gattgaccga cttcttcccg gagtttcctc cgccactatc
    12961 gattgcagcc atctagcccg ttactccgcg ggaaggttct gaaggtcgac cttggagcgg
    13021 ttaccaacga ccaggttgag aacctgcacg gtggccgcat tgatgtcgtc gatgatcttc
    13081 tgggtcttgc cgttgagaag acccatcatc aggagtgcga agaccgcggt gatcagaccg
    13141 aacgcggtgc agttcatcgc ttcctcgatg cccttcgcca gaccggtggc cttgctggcc
    13201 gcgtcggcct tggcaacctc accgaacgcg gtgatgagac cgacgacggt tccgaagaga
    13261 ccgcagagca tgccgacgtt cgcgagcagg ccgagatacg gcgtgcggcg ttcgatcagc
    13321 ggaagctctt tgagcgcagc ttcatccatg gccgcctgaa cttcggcgtc cgggcggttc
    13381 accttgagca aaccctgctt gacgatgcgc gccagcgggt tgtgctctgc ggagcaaacc
    13441 ttgatcgcgc ctgcgacgtc tcccgcagtg atgcacttct gcatctttgc gatgaactcg
    13501 tctttatcga tagacgcttt gtagagatac atcgctcgct cgatcgtgaa tccgattgcg
    13561 aacacgagac acgtgaggat gagccacatc gcccatccgc ctgcttcaaa gtggtatgcc
    13621 agcttggcca ttttttcctg cctcctgcga actttacttc aacagtgtta tccggacccc
    13681 gagtgagcac ggtctgatcc ccagcactgt cttctgacta aaactcgctg ttccccccag
    13741 aacagtaagc aattccaacg tttgcttact acatagggat gataacggtt gggtggagca
    13801 agtcaacatc gtgcccgagt cgaattctta gaaaccgggc cggttcgggc cttgggtcgc
    13861 aaagcggaaa aagctgtagt cgcgttgaca agtttgcagg gcgtggggta gcttgcccct
    13921 cggtcacggg gttcgccccc gacagtcgca atgatttcaa tggattccgg gtcaccgggt
    13981 ccaaatttca gtgaggctga agggaagcga aaaaagaggg gtttctcgcc attcacaggc
    14041 gagccggagg gagccgcaag catgcatgaa atctgggagg cactgctcga aggtgctccg
    14101 ttttcgttca tcaatctcgc tgttttggct tttggtgtgg gcgtgatcat cgaccgcgcc
    14161 tggtacatcc ggatgaacta cagcgtcgac gccgctgaat tcatgaaccg aattcgaaag
    14221 ctcgtgcagg ccgggaacat cgatcgcgcg atccgtcagt gcgaggccaa ggccgtgccc
    14281 ctgctcgagg tggtcaagtc cggtctcacg caggtaaacc gcggcgagga agccgtcatc
    14341 gcctcgatgg aagagaagat gagcgaggtg atccccgcgc tcgagaagcg cacgggttcg
    14401 ctctggacac tcgccaacat cgccactttg atcggcctgc tcggcacgat cagcggtctc
    14461 attcgggcct tcaaggccgt cggcaccatc gatgacccct cgcaaaagac agcactgctc
    14521 tcggccggta tcgccgaggc catgtggaac accttcctcg gcctcttgat cgccgtgatc
    14581 ttcatggtcg ctcacttggt ccttcacgga ctcaccaagc ggcacaaaca cgagatggaa
    14641 aaggtcacga tgcagctcga gaatcttctc acgctgcgcc ggcaaggcgg ttgatcgggc
    14701 ccgaccatgc ccgcacgact gaccccacgc caacgcgcct atgtccggaa acgaaccatc
    14761 gttcccgacg ccgatccctc ggagctcagt gacgagctga acatcgttcc gttcttggac
    14821 atcgtcgtga acatcatcat gttccttctg atgactctga ccacggtcgc gttcttctcc
    14881 caggtcgaag cggcgcttcc ggagtaccgc aagggcggga tcggcaagcg ggccgcggcc
    14941 gatgaggcgc tcaatttgaa tgtcacggtc acgcgcgaag gcgtcgtcgt ctccggctcg
    15001 ggcggcaagc tcgcacgcgg ctgcaccacc accgcgcccg gcaaggtcat caccgtaccc
    15061 gctggaatga acggcgagca cgactggaaa gccctcacca cctgcgtgac ccgggtcaag
    15121 gaacagttcg aggacgagac gcgtgtcact ttgagcgccg accccgaggt tccctacgag
    15181 gacttgatcg ccgcaatgga cgccgtccgc ggcagcgaga gcgagctctt ccccgacgtc
    15241 ttgctctcgg cggggattcg ataatggcca acgagagcag caggccgccc cacgactcgc
    15301 tcgcgccccc ggtgtctgaa aagccggtct cgatgtcgta cgtgaaccgc gtcgtccgcg
    15361 cgaagctgcg acggcgtgcg gagcctgagc acttcgggct caacatctac ccgatgatgg
    15421 acatgctcac gatcctgctc gtgtacatga tggcgatctt cgccacttcg tcagcggcgg
    15481 ccgtccagga aagcagcgag ctccgcatcc cctactccac ctccaaggtc gaggtcagcg
    15541 aagcgatcgg cgtccagatc tcccgaagcg gcgtcgcggt cgacggaaag cccgtgctcg
    15601 agctccgcaa cggcatcgtc gacccgagcc tcaagcaagg cggaagcagc ggctttctga
    15661 tcatgccgct ctacaagacc ctgagcgaaa tccgcgatcg caaaaagcga accgcggcgc
    15721 gcttcgccaa ccagcccttc gtcggcgacg tgcagatcgt cgcggacaag cgaaccccgt
    15781 accgcacact gagcgaagtg atctacacgc tgggtcagac cgagttcaag aacttgcggt
    15841 tcgtggtgaa taagaagagc gagtagtcgc gggcactggc actgggagaa tgacgcttcc
    15901 agctgtaccg atcggagggc ggctcgcgac gggcgtgctc gcgattggga cccgctcggc
    15961 cgtctcccca agtttcccta cgcctagcct cccgcccatc gctgtgctgc ccatcccgag
    16021 ccgccctcct atgcagatcc gacggattaa cactgacgct gacgctggca ctggcactgg
    16081 cgctgccccg gacggcctag agctcgaagt cgccgtactc gaggatttcg gcgatgcggt
    16141 agaggaagcc attggcgtgc actccgtcga cgacgcgatg gtcgtaggtg agcgccatgt
    16201 gcatgaccgg acgaatcgcc atcaagtctt cgccctcgtg ctcgatgacg accacgcgct
    16261 tcttgatggt gcctaggcga aggatggcca cgttgggctg cgagatgatc gcgccgccga
    16321 ccagattgcc tcggcgtccg ggattcgaga tggcgaaggt cttgcccgag agatcgttcg
    16381 gcgtgagatt gccggtgcgg gctcgttccg cgacgtctgc gaccgcacgg gccacgccgc
    16441 gaatcgtgag gtcatcggta tttcgaatca ccggcaccac gagaccttca tccgtgtcga
    16501 ccgcgattcc gatgttgact tcggcgagct tgacgaacgc gtcatcgagc acgcgggagt
    16561 tgatctccgg gaactcccga agcgcgcgcg cggtcgctgc gatggcaaag gccaaatacg
    16621 tgaggttgac gccgtctttc ttgaagtggg ccttgtgctg ttcgcgaagc acggccgtgc
    16681 ggtgaaggtc gcactcggcg aaagtcacga cgtcgggcgc ggtctgcttc gagtagacca
    16741 tgtggtcggc gatgatccgg cgacgacgcg tgaagggcac gagctcgtcg ccctctttcg
    16801 gaacgtaggg gggcaggcga aacgcgcccg acggaccggt cccgcgggac ggcgctgggg
    16861 ccgggcgttg cggagcggga ggcgcagagg gcctcgcagc cggttcgggt ggaggtggcg
    16921 gcggtgtcgc catccgctcg ggggcggttg agctgccgat cgccgccatg acgtcgtcct
    16981 tgacgatgcg gccgccatca cccgagcctc ggacgctcga gagatcgacg tccttctcgc
    17041 gtgcaagctt gcggactgag ggtgaggtcg gcgaaggagc gtcggaaact gcagccttgg
    17101 gcgcctgctt ggtcttcgta ggtggagtga cttccgcctt gccgctctcg tcgatctcgc
    17161 aaatcgcatc acccacggcg acgatgtcgt ccgcggatgc caagagctgc gtgactacgc
    17221 ccgcgacggg cgaaggcagc tcactgtccg ctttatcggt gaggatctcc acgagaggct
    17281 cgtccttggc gacggattgc cccacggaga cgagccattt gccgatcgtg ccctcgacga
    17341 cgctctcgcc gagttgcggc atggtcactt tggtggccat tcgggcagga acatagccca
    17401 aaaagcccgg ggcggtagcg tcttcgagaa cttgttctgc gcatggcgtt cttttcagcc
    17461 atcgagatgt cctcttccga ctagtgttga gttgcgggtc ggagtagagt ccggcgcatg
    17521 ctggatgctc tcaaccggga agacaggctt caattgatga ggtttgtgtg ctccttcgcc
    17581 tgggccgacc tcgaggtcag cgacgaagaa cgcgagttca tcgtcaagat ggtcatccga
    17641 ctgggcttga acgaggagga gcaggatcag gtcgcccagt ggctcgaggt tccgcctcgc
    17701 gccgacgacc tcgaccccgc cgacatcccg agggagcatc ggaagctgtt cctagacgcg
    17761 gcaaaagcca tgatcctatc ggacggcacc gtcgaggacg cagaggcgga gaacctgatc
    17821 attctcgacc agttgctgcg ctagcacccg ctcaagcgca agggtcgaga agctctgaaa
    17881 cggccgggcg tgcggcggcc aagctcgcgc cgaggctgac gacctgcatc gggtcgaggt
    17941 cgaaacgggg ccgcttgccg aagtaggatt cgatgtaacg gcgaagaccg ggaagaagcg
    18001 tgctgccgcc cgcgaggaag accgcgtcca tgtcgcgggc cttgacgccc gcttgagcca
    18061 gaacctcatc gcagatcacg aaggtgcggc gtaccaagtc cagcgtgagt tgctgaacct
    18121 cttctcgggt gatcgaaatg acaccgcagc tttcgggagc tgcaggatcg acgctcggaa
    18181 tcgcaatcgc cgcttgctcg gactcgctga gctcacactt ggcgagctcg caagcgagaa
    18241 ccagccgatc gaagatgacc gcgtcgttac tgagatccca accgtctcgc gcaagcgtcc
    18301 gatcggcgat gtgcagcgcg atggtcctgt ccacgtcgtc gcctccgagg tacgggtcac
    18361 cgccgtgacc gatgacgcgg aagggatagc tgctgcaatc gaccacggcg gcatcaaagg
    18421 tgccaccgcc caagtcgtac acgaacgcat gcttgaggct gcttcgattc aaataagcga
    18481 gtgccgtcgc gatcggctcc tcgatcactc ggaccgaggc gaaccgcgcc tgggaaaccg
    18541 caagggcggt cgcatcccgg cggcgtccat cgaagcccac gggcgccgtc acgaccgcgt
    18601 gcacgtcctc cactggacac ttggtgagtc ggcagagccc tcccaccacg agcgagccga
    18661 tttccacggg cgagaacaag cctaggcggg tcctgaacgc cacaccgccg tctgcggtct
    18721 ccgctagatc gtacggatag tggtgacgaa actgagacgc ataggatgag cgccaacgag
    18781 cgccgatgat ccgcttggca gagataatgg tattcctggg gtccatcgcg cgccggcgcc
    18841 gcgcagcctg gccgatcagc gtaaccccac ttgggggaaa cgcgacggcg gagggcatga
    18901 cgcaactgcc tccctcgatc gagagggact cgaaattcct agcaatcacc gtattggtgg
    18961 tgccgaggtc aattccgatc gtcttctttc tgcggagaga catcggtcga ccggaagtct
    19021 ggggtctgag gttggtgagg tccatggggg gagggagtgg cctctgggaa ataaccaggc
    19081 tacgtgattc agaagctgcc tgccacactc acttttgccg gctggaccga gccccaggtc
    19141 tgttctaaag tgaaacacgt tctagttgga ggcttcggcc catgcccgcg gactttcgca
    19201 agcttcaaat cgcaggaatc attcaggaaa cgcatgacac gcggtcttac gtgctcgcgg
    19261 tccccgagtc gctgcgcgag gagttccgat accgagccgg gcaattcctg acattcgagg
    19321 tgccctggga cggcatgcag ctccggcgtt gctactcgct ctccagtgct cctgaaaccg
    19381 atccctggcc caaggtgacc gtgaagcggg tcgatgaagg gcgggtttcc aactggttca
    19441 acgatcggct gcaggtcggg gactcgattc tcgttcagcc tcccgagggc cggttcgtcc
    19501 tccgctcgaa cgaaggcgat catgggatcg ttctcttcgg tggaggcagc ggtatcacgc
    19561 cggtcctctc gatcctgaag tcggcgctgc ggaagacctc tcgcaaggtg aagctcatct
    19621 acgccaatcg cgacccgcgc tcgatcatct tcagagacga aatcgcgctc tggctcgctg
    19681 agttcccaga tcggctcgag gtcgttcatc atctcgacag cgagaagggg ttcatgtcga
    19741 tcgccgacgt tcaggcacag atccggggct gggagaacgc cgagttcttc gtttgcggtc
    19801 cgaccggcta catggacacc gtcgaggagg ccttcaaagc gtcccagatc gacacagggc
    19861 agaccaagtt cgagcgtttc atttcgccgg tcgatccgga tcgcagggat gcatccgccg
    19921 ctccggttcc cgagcaaagc gccgatgaga tcccggactc cttcaagatg cagctcgaag
    19981 ggcattctct cgaggttccc tatgaaaagg gtctgaccct tctcgagtcg gccgtgaaag
    20041 ccggccacaa gccgccctcg tcctgcgaag acggctattg cggctgctgc atggcgctcc
    20101 tgaagagcgg ctcggtcaag atgagcaatc acgacgctct cgagccgagc gacatcgagc
    20161 gcggctgggt cctcgcctgt caggcgcgac ctgcgagccg ggagccgatc gaggtcgact
    20221 tcgacgccga gtactaggga cgctggcggt cgggccagtc ccagaaggag ctcttgccga
    20281 tcgtccagtc cgggccgctg ccccagtcct tgcgcgtccg gttctttcgg tgttcgggac
    20341 cgtaggtctt cagcgcagcg ccgtacgcct gccactgctg aatcccgagt ttctcagccc
    20401 agtagtcttc ctcggaggac cactggttgt tgcccgcgta ctgcagaacg gtgattccgg
    20461 ggaaatccat cgcgccctcg ccgctcggat ggtcacgccg gttgatcatg tgaaacacga
    20521 cgcggccgct cggatccgcc atatgccagt cgtagacgcc gtagatttcg ccgtactcct
    20581 ccatgagcgg aacgatccac gcacgaacgg tttcgcgtcc acgcatcgtg ccgagtacgc
    20641 gctcgtaata gacgacgtct tcggtgaaga gatccgccca ggcgttccag tcttcccgga
    20701 tcatcccggt ctgccagaag tgccgaaacg cctcttcgac ctcctcgagc ggatacgaag
    20761 gcactactct gcggcctctt tcccctcggg ccagcagtag aactgcgtgc accaacggcg
    20821 gaagatcccg atcggtccgt ctccgtcaca gagaagtggc ttttcatact ggatcttgtt
    20881 ctcccagatg gggatgtcct gctcgagctg gcgcgtcacc tccgcgacga aggccttgcc
    20941 cacgccccgc gccatctcgg caccggcggt cttcttcacg cagaagtgaa agcgaaggtg
    21001 cacgtggtcg gcgtcgatcg gggtggtcga tgccacgttg agcgtttcga cgatgccttt
    21061 gaagcgaacg acggcaagac cgaagccgta gttgaggctc tcgatcgacc cgtcgaccgt
    21121 gccgcgtggc gtttccatgc cggcgcctga gaagacgcgg aggatcggac catcgatcgt
    21181 ggcctcggac ttcggcatgt tggtcgtgcc gtgcaagtag tgaaagtgag ccgaatcgac
    21241 ggcgttctcg gccatctctt gattgcgcga acggatcgtc cactcgcggt ccacgaactc
    21301 ggtccactcc tcgttgcgga cgccatcttt ggtgccccat tcgggaatct catcggggat
    21361 ctcgaaagtc ggaggctctc cctcggcgtg gtgccaacac atcaccatgt tggcg
//
LOCUS       DASRZF010000200         4643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2200954, whole genome shotgun sequence.
ACCESSION   DASRZF010000200 DASRZF010000000
VERSION     DASRZF010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2200954"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..287)
                     /locus_tag="VFG22_09000"
                     /pseudo
     CDS             complement(6..287)
                     /locus_tag="VFG22_09000"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
     gene            complement(478..1281)
                     /locus_tag="VFG22_09005"
     CDS             complement(478..1281)
                     /locus_tag="VFG22_09005"
                     /inference="COORDINATES: protein motif:HMM:NF016972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HET6416420.1"
                     /translation="MSITRSLLASDLDGTLIPLERDAKRLREVAELVAAFEDVESLDL
                     AYVTGRHLSLGLEGIAEVGLPLPRWFVCDVGTSVYQRNNGAFEADEKYRGVMRDAFGG
                     LRGSEVRSAIGQIDGLQLQEEEKQGEFKVSYYTEGRPERLFPLIESRLAEVDAEVNLV
                     ASHDPVGDRGLLDVLPAGIAKDCAVRYLHDASGVQEKNLVYAGDSGNDRAAMLAGYRT
                     IVVGNASSALKADLARESAARGIGERIYFAEHPYARGVLEGLRHFGLLP"
     gene            1345..4416
                     /locus_tag="VFG22_09010"
     CDS             1345..4416
                     /locus_tag="VFG22_09010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET6416421.1"
                     /translation="MHRFHKRPVIFGEVLFDHFPDGSRILGGAPFNVAWHLRGFNSNP
                     LVISAVGDDAEGREVIERMTSWDLMTHGLQVDAGHPTGRVVASMVDGENHFEIAPDQA
                     WDFIRANEAVEATSDAPTGLLYHGSLALRSEQSWQTLKRLKDEARSRSFVDINLRAPW
                     WTKDEVDWCLSQGDWVKLNDKELALLTGRPTDSFEDCRDAAFALSRMHGNDGVIVTRG
                     SKGAISVLDDTTAFEAKAAPLANLIDTVGAGDAFSAVMCLGILQEWDQQVTLDRAAAF
                     AADICGIRGATTTDFELYERHLSEWAKDTSAGSIRAPGGRELYILSLTIHGLVRATEI
                     ELGRDADTGGQVSYVVDQARALAACPDVKRVDVVTRLIEDRRVDESYSRPFEPICEGA
                     QIVRIPFGPRRYLRKETLWEHLDSLLDQLTRYVRMQERAPDLIHGHYADAGYVGSRLA
                     KLLGVPFVFTGHSLGRVKQLRLAAKGDVSEQTYRFTQRIEAEERTLESAALVIASTRQ
                     EVREQYELYDHYQPDRMQVIPPGVDLSRFSPPVPSAPRPPIADELDRFLRDPGKPMVL
                     ALARADERKNFEGLLHAFGQTEGLREIANLVIVAGNRDEIADMSAGPKRVLTQILMLI
                     DRYDLYGAVAYPKHHDSNDVPEFYRLAAASKGLFVNPALTEPFGLTLLEAAATGLPLV
                     ATNDGGPQDIIGTCKNGILVDAMDPKAIGAAISEALGDPERWKRWSKDGLEAAHANYS
                     WDSHAARYVEEVTRVVKGAQPVPVYRQRTKLAGMDRLLVTDVDDTLTGDDAALATLMK
                     RLDSTDAKVGFGIATGRTLNEALALMDQLNLPIPDVLITAAGSELHYGRHMLPDRSWE
                     RQIRHRWDRSEVQRVLGGVPGLERMESSETEYRLRYQLDPERAPSLKEIRRLARKEGL
                     RVTTILDHEIYLDVIPIRASCGFAIRFFCFKWNLEPQRLLVAGDSGNDWDMLSGDTLG
                     VVVSNHTAELERLRGRPRVYFAKSPHARGILEGIDWYDFLGDISVPPEEQE"
     gene            4413..>4643
                     /locus_tag="VFG22_09015"
     CDS             4413..>4643
                     /locus_tag="VFG22_09015"
                     /inference="COORDINATES: protein motif:HMM:NF013058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416422.1"
                     /translation="MIEALEDLLNTHREPTYLLFRRLKALDRQFLLWSDLVHEFEDFS
                     ASDVGASLLETKMSWIMRHTQEAVVDEPFVCLA"
BASE COUNT          919 a         1462 c         1396 g          866 t
ORIGIN      
        1 gagccctagg gcgacggtct tccctgcacg gtgatcgtct cgcagatctc ggacctgggc
       61 acgccgcttt cgaccgcgcg tcgaactggg ccgattcgtc gatcgatggc gaacgaccag
      121 gtctgcagcg gaaagcccgc catgaagcga ccgttcatga agtacgatgg cgttcccggg
      181 atgttcagct tctgaccaag tgcgtattcc ccgtcgaggc gcgcggcgta cttgtcgctg
      241 tcgagcgctt ccctgaactt cttcatgtcg cgaccgagct ctttcgcgag acgatcgtca
      301 tcagcgcgtc gtccgggccc atttgcggct cgtcgccact gaggggcacc gggttagagg
      361 acccgtgggt cgagctcccc tggcgccgct tccaagatgg tcttggcggg ctccacgggc
      421 tcagatgcga tggtaaacgc cggtttcatc gaagctcgtt tcatgggttt caagtcctca
      481 aggtagcaat ccaaagtgcc gaaggccttc taaaacgccg cgcgcatacg ggtgctctgc
      541 gaagtagatc cgctcgccga tgcccctggc cgcagactcg cgagcgagat cggccttgag
      601 ggctgagctc gcgttgccga ctacgatggt gcggtagccg gcaagcattg ccgcgcgatc
      661 atttcctgaa tctccggcgt aaaccaagtt cttctcctgg accccggagg cgtcgtgcaa
      721 atatcgaacc gcgcagtcct tcgcgatacc cgcgggcaga acgtccagaa gcccccgatc
      781 gccgaccggg tcgtggctcg ccacgagatt cacctcggca tcgacctcag cgagccgtga
      841 ctcgatgagg ggaaacaagc gctcgggccg gccctcggtg taatagctca ccttgaactc
      901 tccttgcttc tcttcttctt ggagctgcaa tccgtcgatt tgtccgatgg cagagcggac
      961 ctccgagccg cgcagccctc cgaatgcatc gcgcatgacg ccgcgatatt tctcgtcagc
     1021 ctcgaacgcg ccgttgtttc gctggtaaac gctcgtgccg acgtcacaaa caaaccagcg
     1081 cggcagcggg agcccaacct ccgcgattcc ttcgagtcca agcgacagat ggcggccggt
     1141 gacgtatgcg aggtccagac tctcgacgtc ttcaaatgca gcgacgagct ccgcaacctc
     1201 gcgcaatcgt ttcgcgtcgc gctccaacgg aatgagggta ccatctagat ctgacgcgag
     1261 cagtgaccgt gttatgctca tcgagacgtg agcctagcga ggactgaacc ctcctgcatc
     1321 aaggaaagcg tcagctgaga acacgtgcac cgttttcaca aaagaccggt cattttcggc
     1381 gaagtgctgt tcgatcattt cccggacggc agccgcattc tgggaggcgc gccgttcaac
     1441 gttgcctggc acctcagggg attcaactcc aatccactcg tgatcagcgc cgttggcgac
     1501 gacgccgagg gccgcgaagt gatcgagcgc atgaccagct gggacctgat gacgcatggc
     1561 ttgcaagtcg acgccgggca cccaacgggc cgcgtcgtgg cgagcatggt cgacggcgag
     1621 aaccacttcg aaattgcgcc cgaccaggcc tgggacttca tccgcgcgaa cgaagccgtg
     1681 gaggcgacat ccgacgcccc gaccggtttg ctctatcacg ggtcgctcgc gcttcgttcc
     1741 gagcagagct ggcagacgct caaacgccta aaagatgaag cacgaagccg atcgttcgtt
     1801 gacatcaacc tccgcgcgcc ctggtggacc aaagacgagg tcgactggtg cctatcccag
     1861 ggtgactggg tcaagctcaa cgacaaggag ctcgcgctcc ttaccggcag gccgacggac
     1921 tcattcgagg actgtcggga cgcggcgttc gcgctgtctc gaatgcacgg caacgatggg
     1981 gtaatcgtca cacgcggatc gaagggagcc atctcggtcc tcgacgacac cacggcgttc
     2041 gaggccaaag cagccccgct cgcaaatctg atcgatacgg tcggcgcggg ggacgcgttc
     2101 agtgccgtca tgtgccttgg cattcttcag gagtgggatc agcaggttac gctggaccgt
     2161 gcagctgcat tcgcggctga catttgcggg atccgcggcg cgacgacgac cgacttcgag
     2221 ctttatgagc ggcacctgag cgaatgggcc aaggatacga gtgcgggctc gatacgcgca
     2281 ccagggggtc gcgagctcta catcttgtca ctgaccattc atggcttggt tcgggcgacc
     2341 gaaatcgagc taggtcgtga cgcggatact ggcggacagg tctcatacgt cgtcgaccag
     2401 gcgagggcgc ttgccgcgtg ccccgacgtc aagcgcgtcg atgtggtgac ccgtttgatc
     2461 gaggaccgac gcgtcgacga aagctactcc cgccccttcg aaccgatctg cgaaggggcc
     2521 caaatcgttc gaatcccttt tggtccgcga cgctacctcc ggaaggaaac gctctgggaa
     2581 catctcgaca gcctcctcga tcagctcact cggtacgtcc gcatgcagga gcgcgctccg
     2641 gatctcatcc acggacacta cgccgacgcc gggtatgtcg gctccaggct agccaagctc
     2701 ttaggcgtgc ctttcgtgtt caccgggcat tcgctcggac gtgtcaaaca actgcggctc
     2761 gccgcgaaag gcgacgtcag cgaacagacc tatcgcttca cccagcgaat cgaggccgag
     2821 gaacgcaccc tcgaaagcgc ggcactcgtc atcgcgagca cgcgccagga ggttcgcgag
     2881 cagtacgagc tctacgacca ctatcagccc gatcgtatgc aggtcatccc accgggcgtc
     2941 gacctgtcgc gcttttctcc ccccgtcccg tccgcgcctc gtcccccgat tgccgatgag
     3001 ctcgaccggt tcttgagaga ccccggcaaa ccgatggtcc tcgcgctggc ccgggcggat
     3061 gaacggaaga actttgaagg gctgcttcat gccttcggac aaaccgaagg cctgcgggag
     3121 attgcgaatc tggtcatcgt cgccggcaat cgagacgaga tcgccgacat gagcgcgggt
     3181 ccaaagcgag tcctgaccca gatcttgatg ctcatcgatc gctatgacct atacggtgcg
     3241 gtcgcctatc ccaagcacca cgactcgaac gacgttccgg agttttatcg gcttgccgca
     3301 gcgagcaaag gtctgttcgt gaacccagcg ctgactgagc ccttcggcct cacgctgctc
     3361 gaagctgcag ccaccggcct tccgctggtc gcgacgaatg acggtgggcc gcaagacatc
     3421 ataggcacct gcaagaacgg cattctcgtc gacgcgatgg accccaaagc gatcggagcc
     3481 gccatcagcg aggccctcgg cgaccccgaa cgctggaaac gatggtcaaa ggatgggctc
     3541 gaggccgccc atgcgaacta ctcttgggac agccacgcgg cacgctacgt tgaagaagtc
     3601 actcgggtcg tgaaaggcgc gcagccagtg cccgtatatc gccaacgtac caagttggcc
     3661 ggaatggaca gactgctcgt cacggacgtc gacgacactc tgacgggcga cgacgcagcc
     3721 ctggcgacgc tcatgaagcg cttggactcc accgatgcga aggttggatt cggcattgca
     3781 accggtcgca cactgaatga agcgctggcg ctcatggatc agctcaacct gccaatcccc
     3841 gacgtgttga tcaccgcagc cgggagcgag cttcactacg ggcggcacat gctccccgac
     3901 cgctcctggg aacgccagat ccgccatcgc tgggaccgaa gcgaggtgca gcgcgtgctc
     3961 ggcggggttc ctggcctcga gcgcatggag tcgtctgaaa cggagtatcg tctccgttac
     4021 cagttggacc cggaacgtgc accaagcctc aaggaaatcc gaaggctcgc acgcaaagag
     4081 gggctgaggg tgacgacgat cctcgatcat gaaatctacc tggacgtgat tccgattcgc
     4141 gcctcctgtg gattcgcgat tcgcttcttc tgtttcaaat ggaacctgga accccagaga
     4201 ttgctcgtgg cgggcgattc agggaacgac tgggacatgc tttctgggga cacactgggc
     4261 gtggtcgtca gcaatcacac cgctgagctc gagcgacttc gcggaaggcc gcgagtctat
     4321 ttcgcaaaaa gtcctcacgc acgcgggatc ctcgagggga tcgattggta tgatttccta
     4381 ggagacatca gcgtcccacc cgaggaacag gaatgattga agcgctcgaa gacctgctca
     4441 atacccatcg cgaaccgacc tatctcttgt tcagacggct gaaggccctc gaccgacagt
     4501 tcctcctttg gtccgatttg gtgcacgagt tcgaggactt ctccgcctcg gatgtgggcg
     4561 cctcgcttct cgagacgaag atgtcgtgga tcatgcgtca cacgcaggag gcggtggtcg
     4621 acgaaccttt cgtctgcctg gcc
//
LOCUS       DASRZF010000201         3593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2889809, whole genome shotgun sequence.
ACCESSION   DASRZF010000201 DASRZF010000000
VERSION     DASRZF010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2889809"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VFG22_09020"
     CDS             complement(<1..590)
                     /locus_tag="VFG22_09020"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HET6416423.1"
                     /translation="MKVVDLRSREARSRTLCGGKGAELAALIDAAMPVPEGFVVTTAA
                     FTEFLEHNGLQSACSELHDADLPQRVELGERIRQGISAGDFPRSIDEQIQRTLVEIEE
                     RVGRRPLWAVRSSAIAEDTDSASFAGQYDTSLGVAASQITEAVRHSWRSSFSERAIRY
                     RALQGTHENRMAVLVQVLVHAETAGVCFTIDPLTGEP"
     gene            complement(587..1621)
                     /locus_tag="VFG22_09025"
     CDS             complement(587..1621)
                     /locus_tag="VFG22_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416424.1"
                     /translation="MLELADEQWHSPTKHRHWNESFYFNFFDQATGWACAARVGVTPN
                     AGERDGFICLYLPDQTTGFIRLTEPIGVLGEEIRAAGIELGCVEPFHTWRIRYDGPIL
                     HFSEPASDADLRRTLDASRPSKHLKLDLEVSAAHPPFDYDERSIRPRPFKEFSRRFAR
                     EKPLRTLQRNFHALLALPSMMGAHHYEQSGTVRGTLEMDGASVKIDGVGQRDHSWGVR
                     DMRAPASWRWLSCQFGAELSFNATQVDLLGMRVQGGFIMHDGTAEALTSWRCEPTYGS
                     SAFWPDAVGLVLTAKSGYQFALEAQVVTPLPVVVKTEREDVLVTAARAKYVWGERVAH
                     GMVEFMEQVS"
     gene            complement(1634..2899)
                     /locus_tag="VFG22_09030"
     CDS             complement(1634..2899)
                     /locus_tag="VFG22_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416425.1"
                     /translation="MNAKRKRVTGLTIALCLLTSNGASANIEPSSYDAKSIGAGTTGM
                     AYLENPSALAINPANLAGIDRGGMSVNITPVFSRSQAPLAGPNSEVTSDLGFGPIPSL
                     FGAVRIADRVVLGFGAYALGIFGAKYSNVQDLYGVSGETGDFQVTFVSGEAAVGVAIE
                     ILSSLDLGVTLRVPFAWQNATIPAELGPTIVPVTATLGGAGWPGGRIGLTWRATPMVA
                     ASLMYRTVATSRLSGELKTLEGLDLSSGVTAKFSTPHVLSGGVAFSLLSGKALTVAAE
                     LHVMFYEASNQRQTLYLDAFAGTELGTAEIPFLWQNAFSVRFGADYQITPRFAVRAGY
                     VPSSSGTTNAGAQPVTPPPAWSHSFSAGFGANWKSFAVDFALQTAFAKAHVGQSSDPD
                     ACSGFIRVGCEGDYKTINGSASVQFVYKR"
     gene            complement(2896..3540)
                     /locus_tag="VFG22_09035"
     CDS             complement(2896..3540)
                     /locus_tag="VFG22_09035"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6416426.1"
                     /translation="MAAPEESVSKGDSRRSQRAAETRLKLLSASAELLAERGYSRLAT
                     AAIAKRAGVAHGTLFKHFATKTELMAATTERVLEEVIGEFLRMAQQFAGQPEPIDHAL
                     NAVWSLFRSERLQATLELYIAARTDDSLRDALRPIFATHRSAFLSAARALLPDSADGA
                     HFESNVTGILATLLGGALLWSVVPEPEFFQSELAFVDRITRAELARLGSQNGER"
BASE COUNT          673 a         1151 c         1119 g          650 t
ORIGIN      
        1 ggctctccgg tcagcggatc gatggtgaag cacacgcctg ccgtttcggc gtgaacaagg
       61 acctgcacga gcaccgccat gcgattttcg tgggttcctt gcagcgcacg gtagcgaatg
      121 gcgcgttcgc tgaacgatga gcgccaggaa tgccgcacgg cctctgtgat ctggctcgcg
      181 gccacgccca gactcgtgtc gtactgaccc gcgaagctcg cgctgtccgt gtcttccgcg
      241 atcgccgatg agcgcacggc ccagagtggg cgtcgcccga cgcgctcctc gatttccacg
      301 agcgtccgtt gaatctgttc gtcgatcgag cgagggaagt cgccggccga gattccctgc
      361 cgaatccgtt ccccgagctc cacacgttgt gggagatccg catcatgcag ctcggagcac
      421 gcggattgga gcccattgtg ctctagaaac tcggtgaatg ccgccgtcgt gacgacgaat
      481 ccttcgggaa ccggcattgc ggcatcgatc agcgcggcga gctcagctcc cttgccacca
      541 caaagcgtcc gggatctggc ctcgcgcgag cgcaggtcca cgaccttcac gagacctgct
      601 ccatgaactc caccattccg tgggcgactc gttcgcccca gacatatttc gcgcgggcgg
      661 cggtcaccag gacgtcttcg cgttcggtct tcacgacgac cggcagtggt gtgacgacct
      721 gggcctcgag agcaaactgg tacccagact ttgcagtgag cacgagcccc acggcgtcgg
      781 gccagaacgc gctagagcca tacgttggtt cgcagcgcca gctcgtgaga gcctctgccg
      841 tgccgtcatg catgatgaat ccgccctgga cccgcatccc gagcaggtcc acttgtgtcg
      901 cgttgaagct gagctcggcg ccgaactggc aggagagcca gcgccagctg gcaggggctc
      961 gcatgtcgcg aacaccccag gaatgatcgc gctgcccgac tccgtctatc ttcacgctcg
     1021 cgccgtccat ctccagcgtc ccacgcacgg ttccggactg ctcatagtgg tgcgcgccca
     1081 tcatcgatgg aagcgcgagg agcgcatgga agttccgctg cagcgtccgc agaggcttct
     1141 cccgcgcaaa gcgcctcgaa aactccttga acgggcgcgg tcgaatcgag cgctcgtcat
     1201 agtcgaaggg tgggtgtgcc gcgctgacct ccaaatcgag cttcaaatgt ttgctcggcc
     1261 tgctggcatc gagagtccgg cgcaggtcag catcggaagc cggctcgctg aaatgtagaa
     1321 tcggtccgtc gtagcggatg cgccaggtgt gaaagggctc cacgcagcca agctcgatcc
     1381 cggcagcccg gatctcttcc ccgagcacac cgatcggctc cgtcagacga atgaaccccg
     1441 tcgtttgatc gggcaggtaa aggcagatga agccgtcgcg ctctccggcg ttgggtgtca
     1501 ctcccacacg cgccgcgcac gcccagcccg tcgcctgatc gaagaagttg aaatagaacg
     1561 actcgttcca gtgccgatgc ttcgtcgggg agtgccattg ctcgtctgcg agttccaaca
     1621 tcgttcgggg cacctagcgc ttgtagacga actgcacgga ggccgagccg ttgatcgtct
     1681 tgtagtcacc ttcgcagccg acgcgaatga agccgctgca cgcatcgggg tcgctgctct
     1741 gcccaacgtg ggctttcgca aaggccgttt gtaacgcaaa atcgacggcg aagctcttcc
     1801 agtttgcgcc gaatccagcg ctaaaggaat ggctccaggc ggggggcgga gtcaccggct
     1861 gcgcgccggc attcgtcgtg cccgacgagc ttgggacgta tccggcgcgg accgcaaagc
     1921 gtggggtgat ttgatagtcg gccccaaagc ggacactgaa cgcgttctgc cagagaaacg
     1981 ggatctcggc ggtaccgagc tcggttccag cgaatgcgtc cagatagagc gtctgtcgct
     2041 ggttggacgc ttcgtagaac atcacgtgga gctcggcggc gacggtgagt gccttgccgc
     2101 tcagcaatga gaacgcgaca ccgcccgaga gaacatgtgg cgtggaaaac ttcgcagtca
     2161 cgcccgagct gaggtccagc ccttctagag tcttcagctc gccactcaga cggctcgttg
     2221 cgacggttcg gtacatcagc gacgccgcca ccatcggtgt tgcccgccag gtcagtccga
     2281 tgcgcccgcc cggccagccc gcgccgccca atgtggcggt gaccggaacg atcgtcggcc
     2341 caagctccgc aggaatggtc gcgttttgcc aggcaaacgg aacacgcaga gtgacgccaa
     2401 gatcgagcga ggagaggatc tcgatggcca cgccgaccgc tgcctctcct gagacgaacg
     2461 tgacttggaa gtccccggtc tccccagaaa caccgtacag atcctgcacg ttcgaatact
     2521 tcgcgccgaa gattccgaga gcatacgcgc caaagcccag gaccacccgg tctgcgatcc
     2581 gcaccgctcc aaagaggctg ggaatggggc cgaatccgag gtccgaggtc acttcactgt
     2641 ttggtccggc gagcggcgct tgcgaacggg agaagaccgg cgtgatgttc acgctcatgc
     2701 caccgcggtc gatccccgcc aagttggccg ggttgatcgc tagcgcagac gggttctcga
     2761 ggtatgccat cccggtcgtg cccgcaccga tgctcttcgc gtcgtacgag gaaggctcga
     2821 tgtttgccga cgctccgttg gaggtcagca agcagagcgc tatcgtgagg cccgtgactc
     2881 gtttcctctt cgcgttcatc tttccccgtt ctgcgagccc aggcgcgcga gctctgcgcg
     2941 agtgattcga tcgacaaacg cgagctccga ttgaaagaac tctggctcgg gcacgaccga
     3001 ccagagcagt gccccgccaa gcaaggtcgc gaggatcccg gtgacgttgg actcgaagtg
     3061 cgcgccatcg gcgctgtcag gcaaaagggc acgtgccgcg gacaggaatg cgcttcgatg
     3121 ggtcgcgaag atcggacgga gggcgtcgcg caaggaatcg tcggtgcggg cggcgatata
     3181 cagctcgagg gtcgcctgaa gacgctcact tcggaacaag gaccaaaccg cgttgagcgc
     3241 atgatcgatc ggctccggct ggcccgcgaa ctgctgcgcc attctcagaa actccccaat
     3301 cacttcttcc agaacccgct cggtggttgc tgccatgagc tcggtcttgg tggcaaagtg
     3361 cttgaacaga gtcccgtgtg cgacgcccgc gcgttttgct atcgcggcgg tcgcaaggcg
     3421 gctgtagccc cgctcggcca gcagctcggc cgaggcgctc agaagcttca gtcgcgtctc
     3481 cgcggcccgc tggcttcgac gagaatcgcc tttcgaaacc gactcttccg gggcggccat
     3541 gaaagtgagt atctactcac tcacgaagaa agtctatcct gctttgcacc caa
//
LOCUS       DASRZF010000202         2314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2545428, whole genome shotgun sequence.
ACCESSION   DASRZF010000202 DASRZF010000000
VERSION     DASRZF010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2545428"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VFG22_09040"
     CDS             <1..247
                     /locus_tag="VFG22_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416427.1"
                     /translation="EVVAEFDDSALMKTFGQDGVGVFCSPSVMEAEVKRQYGVQVVGR
                     ADDIRERFYAISPERRLKNPAVVAICESAREELFAKR"
     gene            complement(305..1438)
                     /locus_tag="VFG22_09045"
     CDS             complement(305..1438)
                     /locus_tag="VFG22_09045"
                     /inference="COORDINATES: protein motif:HMM:NF015012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-dopachrome tautomerase-related protein"
                     /protein_id="HET6416428.1"
                     /translation="MAKLFLRVLLVLLGIIAVLAITLLIRYGGGEPYPDLSGPPLFSE
                     DALEVVVTSPEPIGNVAVSADGRVFYTIHPESRPSGPKLVEWVDGAPKPFPSLEAQAL
                     FETPLGIVVDRQNRLWTIDHGNHGTGTARLLAFNLSTGAVVHSHDFHASVAELGSFLQ
                     DLQVDSTGRHVFIADVSFWRKNPGLVVYDVETGVARRVLNSDPSVTPQSWIIRNPTKF
                     MEFFGGLVVLKPGVDGIAISRDDQWIYYGAMAHDTMYRIAVADLLDESLSPEALAARV
                     QAVGRKPLNDGLSTDLDGNLLITDVEHGAVMRMNPEGALTTLIRTRRIRWADALSYGP
                     EDWLYVADSAIPDQMLRSKAHMADAAPYHIFRFKPGIAGSPGQ"
     gene            1636..>2314
                     /locus_tag="VFG22_09050"
     CDS             1636..>2314
                     /locus_tag="VFG22_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416429.1"
                     /translation="MKSTHLMSLILLVSGAAACEDPVTLPSGGWGRLVKMLDGPSVYD
                     GPGAEDDVVRGPRLERGAAVYAKVVIGGADLRSADPEIFTVDDVEVREVGEICESDGE
                     CRTDYDTWFTLTGQSVGEADLIVEGPGGDERRLPLSVGEFSSAQIAYVLPQGQFRDSH
                     SDRPTCAQLGHALSEIVVDGALSFEYEWIRFEWLALLVCDDTGTRLLTHATWRLDSDE
                     DAQFINAV"
BASE COUNT          410 a          665 c          786 g          453 t
ORIGIN      
        1 tgaagtcgtt gccgagttcg atgacagcgc gctcatgaaa acgttcggtc aagacggggt
       61 gggggtcttc tgctctcctt cggtgatgga ggccgaagtc aagcgtcagt acggagtcca
      121 agtcgtgggg cgggccgacg acatccggga gcggttctac gccatctcgc ccgaacggcg
      181 cctcaagaac cccgcggtgg tggcgatctg cgagagcgcg cgagaggagc tcttcgccaa
      241 acgttaggag ggagcgttga agtccctcca atcgccatcg aaccaggctg ggattgccgc
      301 cggctcactg tccggggcta cccgcaatgc ccggcttgaa gcggaagatg tgataagggg
      361 cggcgtccgc catgtgggct ttcgagcgca gcatctgatc ggggatcgcg ctgtcggcga
      421 cgtagagcca gtcttcggga ccgtagctca aagcgtcggc ccagcggatc cgacgggttc
      481 taatcaaagt ggtgagcgcg ccttccgggt tcatgcgcat gaccgcccca tgctcgacgt
      541 cggtgatcag aaggttcccg tcgaggtcgg tgcttagacc atcgttgagc ggcttgcgac
      601 cgaccgcttg cacccgcgcg gcgagcgcct cggggctcag ggattcatcg agcaggtcgg
      661 cgactgcgat tcgatacatc gtgtcgtgcg ccatcgcccc gtagtaaatc cactggtcat
      721 ctcggctgat tgcgatgccg tccacgccgg gcttgagaac gaccagaccc ccaaagaact
      781 ccatgaactt ggtcgggttg cgaatgatcc agctctgcgg ggtgaccgat ggatcgctgt
      841 tcagcacgcg ccgcgcaacg ccggtttcga catcgtagac gacgaggccc gggttctttc
      901 gccagaagct cacgtccgca atgaatacgt gccttcccgt ggaatcgacc tgcaagtctt
      961 ggagaaacga gcccagctcg gcgacggagg cgtgaaaatc gtgggagtgc acgaccgcgc
     1021 ctgtgctgag attgaacgcg agaagccgtg ccgtgcccgt cccgtggttg ccgtggtcga
     1081 tcgtccagag tcgattctgc cggtcgacga cgattccgag cggcgtctcg aacagggcct
     1141 gggcctcgag actcggaaac ggcttcggcg ctccgtcgac ccattcgacg agcttgggtc
     1201 cgctcggtcg gctttccgga tggatcgtgt agaacacacg tccgtccgcc gagaccgcga
     1261 cgtttccgat cggttcgggg ctggtcacca cgacttcgag cgcatcttcg ctgaacagcg
     1321 gcgggccgct cagatcggga tagggctcgc cgccgccgta gcgaatgagc aaggtgatgg
     1381 ccagcacggc gatgatcccg agcagcacga gcaagacgcg gaggaagagt ttcgccatgg
     1441 ggcgcacggt agcatggaca tgggtggtct agccgagcgc ttcatggacc tccgtctgtg
     1501 aaccctatgc gtcagcgatg aggtgctgag gtcacacctt gccgcgagct gctgtgagat
     1561 accgtccgtt cctcggtggc atgggcgttg caagtgcctc ttgcgttgaa gcggtgccga
     1621 ggcgggagcg attccatgaa atctactcac ctgatgagtc tgattctgct tgtttcggga
     1681 gcagcggcct gtgaggaccc cgtgactctt ccgagcggag gttgggggcg gcttgtcaag
     1741 atgctggacg gaccttccgt ctatgacggg cccggtgcag aggacgatgt agttcgcggt
     1801 cctcgcttgg agcgcggtgc ggcggtctac gcgaaggtcg tgatcggcgg tgccgacctc
     1861 cgctcagccg atccggaaat cttcacggtg gatgatgtag aagtacgaga agtaggtgag
     1921 atctgtgaga gcgacggcga gtgtcgaacc gactacgaca cctggtttac gttgacgggt
     1981 cagtccgtcg gtgaggcgga tctgatcgtc gaaggccccg gcggggatga gcgtcgtctt
     2041 ccgctgagcg tcggggagtt ctcatccgct cagatcgcct atgtcttgcc gcagggccag
     2101 ttccgggact ctcactccga tcgaccgacg tgtgcgcagc tgggccacgc gctcagcgaa
     2161 atcgtggtcg atggagcgct ctccttcgag tacgaatgga tccgtttcga gtggctggcg
     2221 ctgctcgtgt gtgacgacac gggcacgcgc ttgttgacgc acgcgacttg gcggctcgac
     2281 agtgatgagg acgcgcagtt catcaatgcg gtgc
//
LOCUS       DASRZF010000203         2671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1171826, whole genome shotgun sequence.
ACCESSION   DASRZF010000203 DASRZF010000000
VERSION     DASRZF010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171826"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1646)
                     /locus_tag="VFG22_09055"
     CDS             complement(<1..1646)
                     /locus_tag="VFG22_09055"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF016994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HET6416430.1"
                     /translation="MDTREQRNRMVDRHVEARGVHDPAVLAAMRSVPREAFLTEHLAE
                     FAYEDTPLPIEEGQTISQPFIVAAMVEALKLGPDDKVLEIGTGSGYAAAVLAEVAAEV
                     YTIERHRLLADQADRRLREIGYENVHVLCGDGTLGWPEHAPFDAILVSAGGPDVPRVL
                     VEQLAPRGRLVVPVGPDLRDQRLLRVTKQPDGGTETEDLGGVRFVPLIGAGGFPESVE
                     VPSAKRIPRPEVPVTHLIREACQPVSSIADVDVGPLLEQIGDARVVLLGEASHGTSEF
                     YRMRARITGELVMRRGFNIVAIEGDWPDAARVDHHVRALDFPLREDQPFTRFPTWMWR
                     NREVLELIAWLKSHNDVIEDPDRRVSFHGLDLYSLYKSLAAVLHYLKEVDPKAAQIAA
                     ERYACLSPWERDPALYGRAALSEGYAKCERPVVSVLRDLLERELDYARSDGDRFFDAA
                     SNAHLVAAAEQYYRVMYYGGPASWNLRDQHMFDTLERLLAFRGPKAKAIVWAHNSHVG
                     DARATEMGASRGEHNIGQLCRQAFGDAAFSIGFGTHTGTVA"
     gene            complement(1802..>2671)
                     /locus_tag="VFG22_09060"
     CDS             complement(1802..>2671)
                     /locus_tag="VFG22_09060"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HET6416431.1"
                     /translation="GVFKGKQRVAGRTEEELFKTVGLRYIEPELRENRGEIEAAQKNR
                     LPHLVSLKDIRGDLHAHSSATDGHHSLREMAEAAKSRGYVYLAITDHSKRVTIAHGMD
                     EARLRRQIDAIDRLNATLKGMRILKGVEVDILKDGSLDIRDSVLKRLDVVVGAIHSGF
                     GARRVEQTERIIRAMDNPYFNILAHPTGRLINQRPTYEVDMERVMRAALERGCFLELN
                     AQPDRLDLNDVHCRMAKDLGLKLAIATDAHSTSCLDYMRFGVDQGRRGWLEPGDVLNT
                     RSWRELKTLFARR"
BASE COUNT          491 a          858 c          807 g          515 t
ORIGIN      
        1 gccactgttc ctgtatgggt gccaaagcca attgaaaatg cggcatcccc aaaggcctgg
       61 cggcacagtt gcccgatgtt gtgctcgcct cgcgatgcgc ccatctccgt cgcgcgggcg
      121 tcgcccacgt gcgaattgtg cgcccaaacg atcgccttcg ccttgggccc gcgaaatgcc
      181 agcagccgtt ccaaggtgtc gaacatgtgc tggtcacgaa ggttccacga ggcgggcccg
      241 ccataataca tgacgcggta gtactgctcg gcggcagcga ccagatgcgc gttcgatgcc
      301 gcgtcgaaaa accgatcacc atcggacctc gcatagtcga gctcccgctc gagcaggtcg
      361 cgaagaaccg atacgaccgg gcgctcgcat ttggcgtacc cttcactgag cgccgctcgc
      421 ccgtaaagag ccggatcgcg ttcccaaggc gaaagacatg cgtatcgttc agcggcgatc
      481 tgcgctgcct tcgggtcgac ctccttcaag tagtggagaa ccgccgcaag tgacttgtac
      541 aagctataga ggtcgagtcc gtgaaagctc acgcgtcgat cggggtcctc gatcacgtcg
      601 ttgtgggact tgagccaggc gatcaactcg agcacctctc ggttccgcca catccaagtg
      661 ggaaagcgcg tgaacggttg gtcctcgcga agaggaaaat ccagcgcgcg aacatggtga
      721 tcgacgcgcg cggcgtcagg ccagtctccc tcgattgcca cgatgttgaa tccgcgtcgc
      781 atcacgagct cgcccgtgat gcgcgctctc attcgataga actccgacgt tccatgtgag
      841 gcctcgccga gaagcacgac gcgcgcatcg ccgatctgct cgagcaaagg accgacatct
      901 acgtcagcaa tacttgaaac cggttggcag gcttcgcgaa ttagatgcgt gactgggacc
      961 tcgggtcgcg ggatgcgctt tgcactgggg acctccacgc tttccggaaa gccgcctgct
     1021 ccgatgagcg gaacgaagcg gacaccgccc aggtcctcgg tttccgtgcc cccgtcgggc
     1081 tgcttggtca cgcggagcaa acgctggtcg cgcagatcag gtccaactgg gacgacgagc
     1141 cgcccgcggg gtgcgagttg ttcgacgagt actcgcggca cgtcgggtcc gcctgccgaa
     1201 accaggatcg cgtcgaacgg tgcatgctca ggccatccaa gtgttccatc tccacacaga
     1261 acatgaacgt tctcatagcc gatctcgcgg agccggcggt cggcctgatc cgcgagcagg
     1321 cgatgtcgct cgatggtgta gacctcggcg gcgacttcag cgagcactgc ggccgcatag
     1381 ccagagcccg tcccgatctc caagaccttg tcatccggac cgagcttcaa ggcctcgacc
     1441 atcgccgcga cgatgaaggg ctgtgaaatc gtctgccctt cttcgatggg caggggcgtg
     1501 tcttcgtacg cgaactccgc aaggtgctcg gtaagaaacg cttcccgcgg gaccgagcgc
     1561 atggctgcga gcacggccgg atcgtggaca ccccgggcct ccacgtgccg atcgaccatt
     1621 cgatttcgct gttcacgcgt atccattatg cgtgccttcc gagttcccgc ctcgcgcact
     1681 tgcgcagacc agttcagtga agcaacaagc gggccaagac gatttgttgc gccagaaggc
     1741 cgttggacgc gccgagagag ggacgatttc ggctccgagg cgcagaacct gcgcctcgcg
     1801 ctcatcttcg cgcaaataga gtcttgagct ctcgccacga acgcgtgttg agcacgtcgc
     1861 ctggctcgag ccaaccgcgg cggccctggt ctactccgaa gcgcatgtag tccaggcagc
     1921 tcgtcgagtg cgcgtccgtc gcaatggcaa gcttgagacc caggtccttg gccatgcggc
     1981 agtggacgtc attcagatcc agtcggtcgg gctgggcgtt cagctcgaga aagcatcccc
     2041 gttcgagggc ggccctcatg acgcgctcca tgtcgacttc gtaggtcgga cgttggttga
     2101 tcaggcgccc ggtcggatgc gccaagatgt tgaagtatgg attgtccatc gcgcgaatga
     2161 ttcgctccgt ttgctcgacg cggcgcgctc cgaacccgct gtgaatcgcg ccgacgacga
     2221 cgtccaagcg cttcaggacg ctatcgcgga tgtcgagcga cccgtctttc aggatgtcga
     2281 cctcgacgcc cttcagaatg cgcattcctt tgagagtggc gttcaagcgg tcgatcgcgt
     2341 cgatctgacg ccgaagccgc gcttcgtcca tcccatgcgc aatggtgacg cgtttcgaat
     2401 ggtccgtgat cgccaggtac acgtagcccc gactcttggc cgcctcggcc atctcgcgca
     2461 gcgaatgatg gccgtcggtc gcgctgctgt gggcatgcag gtcaccacgg atgtccttca
     2521 gcgaaaccaa atgcggcagc ctgttcttct gcgcggcttc gatctcgccc cggttctccc
     2581 gcagctcggg ttcgatatag cgaagtccca ccgtcttgaa gagctcctct tccgttctac
     2641 ccgccactcg ctgtttgccc ttgaagacgc c
//
LOCUS       DASRZF010000204        28318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2203443, whole genome shotgun sequence.
ACCESSION   DASRZF010000204 DASRZF010000000
VERSION     DASRZF010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 28318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28318
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2203443"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..258
                     /gene="rpsR"
                     /locus_tag="VFG22_09065"
     CDS             70..258
                     /gene="rpsR"
                     /locus_tag="VFG22_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005705387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HET6416432.1"
                     /translation="MGLPPDFKFDYKDPQQLRNFITDRGKITPRRISGLNAKQQRDLT
                     RAIKRARNIALLPYTATK"
     gene            266..733
                     /gene="rplI"
                     /locus_tag="VFG22_09070"
     CDS             266..733
                     /gene="rplI"
                     /locus_tag="VFG22_09070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HET6416433.1"
                     /translation="MASTVEVVLKDNLEHLGSIGDVVRVRRGYARNFLLPRGLAVVAS
                     RGNVRQIEHEKAIQAQRIAKLRSEQEKIVAELAKVTVMIAKEAGEDGKLFGSVTSNDV
                     IDGLIAKGVDVEIDKRKLVMPPQPIKEVGSYEVGLKLPYGLTAIVKLEVKTRA"
     gene            818..2206
                     /gene="dnaB"
                     /locus_tag="VFG22_09075"
     CDS             818..2206
                     /gene="dnaB"
                     /locus_tag="VFG22_09075"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HET6416434.1"
                     /translation="MSSPQQVGQVPPNSLDAERAVLGGILLENDAMNVVVEVLSASDF
                     YSEANGKIFEAMSELFRRSQPVDHVTLREALVQSGKLASIGGDEYLLSLSNTIPSVSN
                     IQSHARIIREKSVIRSLIRVCHEVASRGYGDYGPVEEFLDQSESSIFAVARERARNPY
                     EHVKDVVMRTFKEIHETANRGEAITGLPTGYKQLDKMTAGMHPGDLIIVAGRPGMGKT
                     SFALNVAHHACQKSKAPVGVFSLEMPKGQLVRRLLGSEARVDGNRIRTGQLHKDDWPK
                     LADAAGSLSEMPIWIDDTPAISMLELRAKARRLKSEVGLSLIVVDYLQLMRSGSRNDS
                     REQEISEISRSLKALAKELEIPVIALSQLNRGVESRGVKDKRPQLSDLRESGAIEQDA
                     DTIWFIYRDEVYNRESDDRGIAEIIIGKQRAGPTGTCRVRFFNEYTRFDDLAESDYYG
                     GASSGIADFQDN"
     gene            complement(2248..3681)
                     /locus_tag="VFG22_09080"
     CDS             complement(2248..3681)
                     /locus_tag="VFG22_09080"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HET6416435.1"
                     /translation="MNHEIMRSTVVPIIYLLSTVLFIFGIKQLSKVKTARRGNALAAV
                     GMLLAIIGALLELGLVDYRWIAGGILLGTIIGAIFAIRVQMTSMPEMVALFNGFGGIA
                     SALVATSVYWDKIVEQAPVSRVYQTLGTPEAVTVMLSILIGGITFTGSLIALFKLQGT
                     LKNGQPILLPARHYITFALLFGSFAFGGIAVAATASDSLVWVLVLGAVSLLLGVLLTI
                     PIGGADMPVVVSLLNSYSGVAASMAGFVIGNPLLIIAGAMVGAAGLILTQIMCVAMNR
                     SLLNVLVGGFGVTDGGGGGGSDEYTTVTSCGPEEAAMILENAESVIIVPGYGLAVAQA
                     QHAVRELADMLKARGTKVTYAIHPVAGRMPGHMNVLLAEADVPYEELLEMDAINPEFK
                     NTDVVIVLGANDVVNPIASKDPTSPIAGMPILNAHEARSVFVVKRSLSPGYAGIKNEL
                     FEYPNTMMIFGDAKKVLQGLIAEVKAL"
     gene            complement(3678..4031)
                     /locus_tag="VFG22_09085"
     CDS             complement(3678..4031)
                     /locus_tag="VFG22_09085"
                     /inference="COORDINATES: protein motif:HMM:NF024179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HET6416436.1"
                     /translation="MTLGPLLIGIYVFVLASFVGFEMITKVPPTLHTPLMSGANAISG
                     ITVVGAVAAATTGAPTVANILGAIAIVTATINVVGGFLVTDRMLRMFGKKKKPKALEP
                     DSKGVTAGEREAVSK"
     gene            complement(4047..5180)
                     /locus_tag="VFG22_09090"
     CDS             complement(4047..5180)
                     /locus_tag="VFG22_09090"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HET6416437.1"
                     /translation="MPQVFVPKEIANGETRVACTPETTKRYVKEGFDVVVESEAGKYA
                     HFTDQHYLDAGAKIVSGAEAAAAWGSADIVFRVYPITPEEASKLKQGALLIGFMSPHR
                     NLESVKILRDRKVSTVAMELIPRISRAQSMDALSSQASISGYQAVLMAATYLDKYFPL
                     LMTAAGTIQPAKIVIMGAGVAGLQAIATARRLGAVVEVSDIRPSVKEQVESLGGRFID
                     FPMDQSGEGEGGYARELTAEQLKAQQAIVRKKVVAADAVITTALVPGRPAPKLITKDM
                     VEEMREGAVIVDLAVEQGGNCELSRNGEIVVEHGVTIIDYPNLPAATPADASLLYSRN
                     ILALLLSTVTDGKVVLNLEDEILDGALLTHNGEVRHGPTAEAL"
     gene            5372..7372
                     /gene="tkt"
                     /locus_tag="VFG22_09095"
     CDS             5372..7372
                     /gene="tkt"
                     /locus_tag="VFG22_09095"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET6416438.1"
                     /translation="MGNPAKLDELAIKTIRGLSMDAVQAANSGHPGTPMGLAPLGWAL
                     FSELRRHDPAHPDWPDRDRFVLSCGHASMLQYALLHLSGYAVSLDDIRKFRQWGSITP
                     GHPESHVTPGVETTTGPLGQGVGNGVGMAMAERHLAARFNRPGHTVFDHRTWVIASDG
                     DIMEGVASEAASIAGHLKLGKLLVFWDDNRITIDGRTELCFSEDVLARFASYGWHTES
                     VDDGEDLAALVTAAHAAVADARPSFIRVRTIIGRPSPNKQDTSAAHGAPLGEEEIIAT
                     KQMMEWPTEPFFVPDEIVNATEAIRDRGAKSYGAWQRRFESYRAEFPDQAAELESVMA
                     GELPAGWDEDLPAFEADPKGIPTRKASGAVLNALAAKIGGLVGGSADLAGSNNSHMKG
                     LPSFLPGAEGIPRNVDWGIREHGMAAALNGMALHGGVIPYGATFLVFSDYMRPSVRLA
                     ALMKLHTRYIYTHDSIGLGEDGPTHQPVEHLAALRAIPNLAVFRPADANEVRECWKAA
                     LEWDGPAALALTRQNVAVLDRKILASAEKAKRGAYTLREAGGGGPDVILVATGSEVEI
                     ALAAADVLEQEGAAVRVVSMPCWELFARQDEQYQREVLPRAGAKVVIEAGIRQGWDRW
                     VGNEAAFVTIDRFGASAPYEVLYEKYGITAEHVVAEAQRLLS"
     gene            complement(7369..7866)
                     /gene="tpx"
                     /locus_tag="VFG22_09100"
     CDS             complement(7369..7866)
                     /gene="tpx"
                     /locus_tag="VFG22_09100"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HET6416439.1"
                     /translation="MATTALKGTPVQTNGDLPAPGSTAPDFLLVGSDLSEKRLKDFAG
                     KKKILTINPSYDTPVCQATARTFNERAASAKNVVVLVISSDLPFAQKRFCAAEGLEGV
                     IPLSTFRGSFLDDYGVRMVDGAMKDLAARAIVVVDENDKVVHTELVSDIVKEPDYDAA
                     LTAAR"
     gene            7965..8204
                     /locus_tag="VFG22_09105"
     CDS             7965..8204
                     /locus_tag="VFG22_09105"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HET6416440.1"
                     /translation="MKPSLVDLPFGQHAEIVSIDCGRSLSRRLMEMGLLPGTRVRVVR
                     VAPLGDPIELRLRNYSLSLRRAEAAKIAVRVEGAA"
     gene            8201..10369
                     /gene="feoB"
                     /locus_tag="VFG22_09110"
     CDS             8201..10369
                     /gene="feoB"
                     /locus_tag="VFG22_09110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HET6416441.1"
                     /translation="MSAGAPARQVLIAGNPNCGKSTLFNALSGGSAHVGNYPGVTVDR
                     TSARLRSGALNIELVDVPGMYSLTAGSPEEQLAVNALIDEKTAGVICVVDATTLQRGL
                     YLALQLIESGVPVVIALNMMDAAERLGLRIDTERLAALLCSPVIPVVATRRKGLDALV
                     AALENELASSAVPTELGIEYPQTTQADVTKLVPAIAEWRPGTPPEQLRVFALWCLLSL
                     GDDSLRDVPDSVRKAVERTRAVAESEGRNLDLEIISARYAWIDRALKESCSAHDPSPS
                     WSERIDRVLTHPLAGLLIFAVVMLGIFEALFAGAEPLIEGIESLTGFMQAQAAALIPA
                     GVFRDLVSEGIIAGVGNVIVFAPQILMLFLFIGFLEDSGYLARVAFVIDRVMKGVGLH
                     GKAFVPLLSGFACAVPAVMATRTIERRRDRLVTMLALPLMSCSARLPIYILVVGTVFA
                     GQSFGWFSAGAVALFSMYTLSVLVTLAAAAVIRRTVLPGPAPTFVLEMPPYRWPSAQI
                     LWLNAWRRLRIFLVDAGTIILAMTILLWALLSFPKSDDIAQHYAGERQRILETAAAEP
                     KQAEALQSLDSKEAGEQLAFSFGGRFGHAIEPLLRPIGFDWQIGVGIIGAFAAREVFI
                     STLGVVFGIGEADEENKPLRTALREARHADGTPVMTPLSGVSLMVFFLLACQCMSTIA
                     VVRKESGSWKWPVFLFSYMTVLAYIASFLVYQGGQLMGFG"
     gene            10572..11582
                     /locus_tag="VFG22_09115"
     CDS             10572..11582
                     /locus_tag="VFG22_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET6416442.1"
                     /translation="MSRSATIEGLRKFRTKLLRERDPWQDTSRRFRIRRFAGPECFLE
                     HLEGVRVAHLTDLHVGRVTPMAVQYDAVSMTNAGKPDIVVISGDFVCHSQVYLDDLTD
                     VISKIDAPVFAVLGNHDHWAGAEPVRQALRRGGAEVLDNAHTSITIGHQPLQVVGLDD
                     AYTGHADRREALKGLRADLPVIALSHIAEEADALWEEGVPLVFSGHTHAGQVTVARLH
                     ELSVGKLAGHKYIHGLYGSRDAEDKKGAVYVGAGVGAAIVPLRLGERAKREVAFFELG
                     AQPGSFDEHHSEQAAHRGRVPSPKTKERRAQAVLRKQERRERKNGNGNGNGNGKGARF
                     NP"
     gene            complement(11579..12526)
                     /gene="trxB"
                     /locus_tag="VFG22_09120"
     CDS             complement(11579..12526)
                     /gene="trxB"
                     /locus_tag="VFG22_09120"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HET6416443.1"
                     /translation="MSHRVLIIGSGPAAHTAAIYAARAELKPVLFEGFMAGGIAAGGQ
                     LTTTTDVENFPGFPEGIMGPELTDRFRAQSQRFGTTIHTETINEIDFSSRPFRFKADA
                     QEGSADCVIIATGATAKRLEIPGANDGELWQKGISACAVCDGALPAFRNQPLAVIGGG
                     DSAVEEASFLTKYGSTVYIIHRRDELRASKIMQQRALDNPKIQMLWSQVVTQAKGADF
                     LEAIRVKNLKTDEERDIPVAGLFFAIGHTPNTAFLNGQIDTDEQGYIVTKPDSTQTNI
                     PGVFAAGDVQDKKWRQAITAAGTGCMAALEAEHFLGSNS"
     gene            complement(12580..13689)
                     /locus_tag="VFG22_09125"
     CDS             complement(12580..13689)
                     /locus_tag="VFG22_09125"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET6416444.1"
                     /translation="MSEAVDAVVIGAGVVGLACAHELSKRLDSVVVIEREVGPGREIS
                     SRNSGVVHAGIYYPKDSMKTRLCIEGNRRLYQWCEKHDVPHKHTGKLIVATNAEDEQR
                     LRAIARHASEIGAAELRLMTAAEATAQEPELRCALALHSPSSGIVDVHELIASLVHEI
                     KEADGMIVYHTQVDQILKTRDGWLVRTADTTGSEMELEAKRVVNAAGLAALDVAARSG
                     LPETERPWRLYPCKGSYFALGAGAPTTRNSLIYPLPSGGGLGVHITADIAGARRAGPD
                     AEFVETIDYSVDESRAERFAEAVARYLPAIRPEHLTPDYSGIRPKLVGPEGGFADFVI
                     TMPASQPGLVHLVGIESPGLTAALSIGAHVSKLIA"
     gene            complement(13701..16073)
                     /locus_tag="VFG22_09130"
     CDS             complement(13701..16073)
                     /locus_tag="VFG22_09130"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HET6416445.1"
                     /translation="MTQRGIIALLFAAVLFASLARSAHASPDVPMCVADGNNASDPPL
                     SLVQWRAPMCGPYLDAFAIAEAKPPKERAVEAYLAAGDQIAAASYEEARLQLDLARRG
                     LPRIVDRIALQSARLELLRNQPARAAAFFAEAQQSPHESVRFEARYGEVLSLLRADDP
                     GADQLMSHLLDDYPEAPKRSELIFEQVQSLIRRSRYEEAIAVMHAVRVEQPGNRLAGW
                     AEAELNRLETLGYETPSMTGDERVERARRLVGTGPLEQAKVEIAALLESPLTGEQHAE
                     VHYLAGRLARDEGRWQAAETYLRTAQLFPMESLAAQRNVESRASGMAETARARDPKYV
                     QQQLTGLRAGRSNASIPSVRLLDMLKLASAAGLRADTDSILQTLDKRAGVPSPVLFGA
                     AMAAVGTGSEGHIIELLENLVKRPGASYQAAGMYHLARALERSGRLVEAALYFTQAKS
                     TAEAQGDLYYALWASNGLQRLEHGERSGSLEGLSNPEEIDVPRAPRTSNRALAKKLEP
                     IGELHWESYPWIARAADLLRVGEREAATAELFETYLTWRHALGKPIARAGLTCVAQAD
                     SRTQEPIASDVRVARLELSNEERSTLAEVAIALGDMGTAGGFQGPEFIDTLPRAYEWL
                     AVPAANRYGLDPNLLLAVMRVESAYQKHIVSYAGAVGLMQIMPRTGQLIAHALGHDDF
                     TPADLLDPELNLEFAAWYLTSLIRRFDGRLPLAIAAYNGGPHNVRRWIQESPADAPVD
                     VLLERIPFTQTHRYVRKVLTHYEAYRKQRELAMPQLALTIPAQRLDPLAF"
     gene            complement(16164..16358)
                     /locus_tag="VFG22_09135"
     CDS             complement(16164..16358)
                     /locus_tag="VFG22_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416446.1"
                     /translation="MSRGDAVYQTAKWRTLGLALGFAVLAGVGVVTVLLPELADEPDA
                     EEQSSSATSEDLESDTHLQE"
     gene            complement(16363..18084)
                     /locus_tag="VFG22_09140"
     CDS             complement(16363..18084)
                     /locus_tag="VFG22_09140"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HET6416447.1"
                     /translation="MRYSRAFIPTVKEVPKDATMPSHVLMLRAGYARMVGAGIYELLP
                     LGTRVLHKIEQIIREEMDAAGAQELLMPTFVPAEYFKETGRWDLYGQTLLRIKDRKGG
                     EYHLAPTHEEIITDLVRREIKSYRQLPLNLYQIQLKYRDEPRPRAGLLRCREFIMKDA
                     YSFDVSEEAALESYAQMRDTYHRIFTRLGLEYRVVEADSGAIGGKQSAEFQVLAQTGE
                     DWIVACDACDYAANVEVATAGSSQFSASASVGKDLAELEKVETPDTRTIDEVVAFFGG
                     DTTASKTLKSLLYVAGDEVILAIVRGDHEINEIGLARLLGVDEVVLASDAAVKAATRT
                     EIGFVGPVGFKGRIIADPDAVAVRNAIAGAGETPYHYRNVNHGRDFEAEVAPIRQATS
                     GDPCPNCDGRLELYRGIEGGHIFVLGTHYSDKMNASFLDEAGTNRSIVMGCYGIGVSR
                     LVAAIMEQHHDEHGIRWPVQVAPYQVIITPIGKDAEPLAAATEIYEALRADGVEALLD
                     DRPERPGVKFKDADLLGIPVRITVGARGLEQGTVELKKRIESESTDVPVKNAVEAAKA
                     ALAELGA"
     gene            complement(18156..19391)
                     /locus_tag="VFG22_09145"
     CDS             complement(18156..19391)
                     /locus_tag="VFG22_09145"
                     /inference="COORDINATES: protein motif:HMM:NF004936.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HET6416448.1"
                     /translation="MAQVPEVYVLGGYQTDFARNWSKEKKHFVAMMREAVLGALEETQ
                     IEPSEVEVAHTGNFAAELYSKQGHLGAFFLEIDPAFSGIPTARHEAACASGSIALLAA
                     SAEIEAKRYDLACVVGVEQMKTVGPGEGGDFLGTAAWYELEAKGIEFPFPKLFGKLGD
                     EYDKRYGLKDEHLAEISTINYANAKLNPNAQTRTWYMNYDHAMSRDEFNGSIGGRIHI
                     SDCSQVTDGAAAIMVASAEYAKKWADRVGVPLESIPRIKGWGHHTARIQFEDKIEESR
                     NAKYVLPHVRSTVTDAQKRAGIANSDGIDAIETHDCFTTSEYMAIDHFGLTAPGESWK
                     AIEAGDIAFGGKTPINPSGGLIGAGHPVGATGVRQVLDCHKQVSGEAGDYQVEGAKNV
                     QTLNIGGSGTTSVSFIIGV"
     gene            complement(19414..20757)
                     /locus_tag="VFG22_09150"
     CDS             complement(19414..20757)
                     /locus_tag="VFG22_09150"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HET6416449.1"
                     /translation="MLSATPSAAGGKSSDLLSSRSIKHVVVLGANGAMGLGSGALFTT
                     VVPRVTFLARTKEKADGGLAAAVKMVRSSTVAKRVDTGSYDADFDRVVAEADLIFEAV
                     TEQLPLKKEFFDRIDKVRRPDSIVATVTSGLSINLLAEGRSDSFRKNFVGLHFFNPPN
                     VIVGTELIAGKDTDPKLVDFLDEYCTKLLGRAMVRTADTAGFAGNRVGFKVLNECAIL
                     AEQHGPLLVDELVGPYTGRAMTPLATIDLVGWDIHRAIVDNIYELAPDEAHETLKLPK
                     YMAELMAKGVLGRKTGGGFFGKDDAGGRIVLDPKTQNYVSKSSISRPDLGFIDEISNL
                     HRVGRYVDGMRAFAAATGPYAAIAQKVIAGYISYSFNRVGEVTDDISGIDRIMGYGFN
                     WAPPSVLVDTIGLKPTIEMIEKAGLAVPAALANAQEGTTFFDDPQVNVGKFFVAA"
     gene            20960..21997
                     /locus_tag="VFG22_09155"
     CDS             20960..21997
                     /locus_tag="VFG22_09155"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HET6416450.1"
                     /translation="MEAASIANALGISQEAAEIFLSSDAIDLHIESFLWRRIFGYDLN
                     RVHRGGLFGRRFFGHADLPSVQQVGLGGATWVISTNPLRAAGRRRQVFEQNLASLRNL
                     LDEAPGVRHVRNAAEFRAARASGEHAAFIGIQGGNALDHDLDALDVLADQSVLRVTLL
                     HLTSSRLGATSAPGGGRSAGLTDFGKAYVERLNELRIGVDLAHISRPGFFDAVEVHDK
                     TQPLLVTHTGLAGVYEHWRNISDLQLRAVADSGGTVGVMMHAAYLGRGRVTVATLVDH
                     LQHIVATVGEDYASIGTDYDGFIIPPRDLPGIWALPRLVQEMLDRGWSELRIGKILGG
                     NALRVVEALRG"
     gene            22021..22440
                     /locus_tag="VFG22_09160"
     CDS             22021..22440
                     /locus_tag="VFG22_09160"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HET6416451.1"
                     /translation="MALLEKIDADLKTAMLARDEVTRDTLRMIKSELLTLENPDELAV
                     LSRAVKSRRESIKSYVEGGRQDLADKEQAEIEVIERYLPKQLSEDEAREAIAKIIEEL
                     GLSSKKDLGQVMREIKARYPGQVDGKLASSIAAKLLS"
     gene            complement(22446..23645)
                     /locus_tag="VFG22_09165"
     CDS             complement(22446..23645)
                     /locus_tag="VFG22_09165"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET6416452.1"
                     /translation="MGSGNKSDSTYLIKNAPSDRIEAPSPSEAPQTEPPDGRWRTSNP
                     PPGESLEAKWWPDRELGKAFRRWTSIPPARGDDARMMAMKFLVEHLNVGDLSAVGSAP
                     RAPLPTIEPPDFLARVRTMVVRLGMHGPNEEAPDHGTLLAMGEDVLPVLRDFFPGRLW
                     FNRWEPHVQPPAGSSVSGLCRTLVAFGEPAVPFVGELLRSDDPETRYYASLVAAELPH
                     PRLLEPLATALFDDDQGVSRSALYALELFHEQEGIEGLKESLRALAVASDSDQRSRLL
                     AIRALAVLRDEACVEALIPVLSERSVIGEAAWRVLRMLTAQDFGDRAADWHAWLLANR
                     ERSRVEWLIDGLDHPDAEVRAIASRDVVRASGHDYGFKTDMASQDRKAIRERYRLWWA
                     AQSSVQD"
     gene            complement(23672..24733)
                     /locus_tag="VFG22_09170"
     CDS             complement(23672..24733)
                     /locus_tag="VFG22_09170"
                     /inference="COORDINATES: protein motif:HMM:NF039236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET6416453.1"
                     /translation="MMNEPLQHLTEKSVNNGFAPGWYIVAASGDVGAKPKAVSLNGLP
                     LVTFRDSAGQTIALLDRCSHRNAPLSAGRVAGDNIQCHYHGWEFDGAGVCRAVPGLCG
                     SPDHPARRVPAFACRDSQGWVWVYSEADVEPPSAPYRIPCADEPGYLTIRERLEMPGP
                     LDAVAENALDVPHTAFVHAGLFRKNKDRKPIEVVIRALEGGVEAQFIGEQRPEGLAGR
                     ILAPQGGEVFHWDRFLLPCIAQVEYRLSEHSHIIATTALTPRSPTVTDMFATVAAKAP
                     VPNLILRTVFKPVALRILRQDMEILRRQTEQVQRFGAESFASTEIDVLGLRIKKLLRE
                     AASGKAISAQEERRIKMLV"
     gene            24851..25456
                     /locus_tag="VFG22_09175"
     CDS             24851..25456
                     /locus_tag="VFG22_09175"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6416454.1"
                     /translation="MSRQINKERTRRRLLQATLKTIQRYGHGTLTTGRVAQLAGVAQP
                     TFYVHFRSMDQALEQVAEWVAEELSPGLEPEASAELDRAADVLEQAVRKCTRSLVRDR
                     KLAEVFLSNRRDQTSALGRRWTVLTGSLRERMRQIVVRVRPDVTPADAALHAELLVSV
                     IFGLAEAHLDGRVDDLDRATATASRAIVTSILTSSSVADAA"
     gene            25515..26693
                     /locus_tag="VFG22_09180"
     CDS             25515..26693
                     /locus_tag="VFG22_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6416455.1"
                     /translation="MTDLFNPSAEHRALRELVRSFTEREVDPQAEQSDHEERFNVELF
                     RKLGDLGLLGVTVEERFGGAGLDPVAAVIAHEELAAADPGFTLAYLAHSMLFANNLQV
                     NGSDAQRSKYLPDACSGKKVCGMCMSEPGAGTDVLGMQTSAAKHGGVYLLNGAKMWIT
                     NGAISDTELGDTFLVYARIHGSPRNQLSMFIVEKGFPGFSLGQKIKGKLGMRASTTAE
                     LVFENCEVPAENLVGSEGAATLHMMRNLEIERVTLAGMSLGIARRSIEVMNRYASERS
                     SFGKPLRDFGQIQRYIAQSYAEYMAGRAYTYYTAATMSLETPGHRLDSDGVKLFTSTM
                     GKDVADRAIQVLGGYGYVSEYAVERLWRDAKLLEIGGGTLEAHQKNMCRELSHTERIL
                     "
     gene            26844..>28318
                     /locus_tag="VFG22_09185"
     CDS             26844..>28318
                     /locus_tag="VFG22_09185"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6416456.1"
                     /translation="MSGDDVTQDSAASQLPRPPVDLPVLGKFAGYDVLGRLALGGMAE
                     ILLARHANTEGDHRFLVVKRILPHFEQDSDFVQMFLDEARIGMQLKHPNICQFHQFGA
                     DEGSHFIVMEWVNGVPLGRLIRKARNSGGVGLPIAIRIGVDVARALHYAHIANDEHGE
                     PFNIIHRDVSPHNIMIAYTGAVKLLDFGIAKADHRAHKTQAGVVKGKFAYMAPEQCTA
                     GQADHRLDIFALGVCLFETLTGRSLYRRQTEAATMRAVIMDPVPSLKERLPDVSDELD
                     KILQKALAKEASDRYVTAEAFARDLEEFGKANKLFATQREVAEFVKKVFPEEFTRGPR
                     VDTVPFGASITLDMHHANDSSSAKSYVSQIPGAIRELEVDVGGYGDLGHLLDSPVVAP
                     LPLPASEPANSDAQAAPKEVPKRAAPKKRPAGPKKSSGFGWKGILITLLVLLGGVAVA
                     YDQSDIVKDAVGNLSEMFEEKGPPEVSVDDNPLLREHGARIL"
BASE COUNT         5200 a         8655 c         8955 g         5508 t
ORIGIN      
        1 gacattagac aggatttcga cgttgaggcc ggtggccgcc gccgtggtgc acgcaagaaa
       61 gcgccgggct tggggctacc tccggacttc aagttcgact acaaagatcc ccagcagctt
      121 cggaacttca tcaccgatcg cggaaagatc actccccgcc ggatcagcgg tctcaatgca
      181 aagcagcagc gtgacctgac ccgtgcgatc aagcgagctc gcaatattgc gctgctcccc
      241 tacacggcga cgaagtgagg tagccatggc atccacagtg gaagtcgtac taaaagataa
      301 cctggagcac ttgggctcca tcggcgacgt ggtgcgtgtg cgccgcgggt acgcgcggaa
      361 cttcctcctg ccccgcggcc tagcggtcgt agcgagccgg ggcaatgttc gccagatcga
      421 acacgagaag gcaatccagg ctcaacgcat cgcgaagctt cgctccgaac aggagaagat
      481 cgtcgctgag ctggcaaaag tgaccgtcat gattgccaaa gaagccggcg aggatgggaa
      541 gctcttcggt tcggtcacct cgaacgacgt catcgacggg ctcattgcca agggcgtgga
      601 cgtggagatc gacaagagga agctcgtgat gccgcctcag cccatcaaag aggtgggcag
      661 ctacgaggtt ggcttgaagc tcccatatgg cttgaccgcc atcgtgaagc tcgaagtcaa
      721 gacccgcgcc tgagccctaa aagccgacgc ctccattgcg gggcttccgc tcgtccgccg
      781 taggagctag aaccaaagct ctgaagggag cagtacgatc agttcaccgc aacaagttgg
      841 ccaggttcca ccgaactccc tcgacgcgga gcgcgcggtg ctcggcggta ttcttctcga
      901 aaacgatgcg atgaacgtcg tcgtcgaggt gctatccgcc tcggacttct acagcgaagc
      961 gaatgggaag atcttcgaag cgatgagcga gctctttcga cggagccagc ccgtcgatca
     1021 cgtcaccctg agagaggcgc tcgtccagag cggtaagctc gcatcgattg gcggcgacga
     1081 gtacctgttg tcgctgagca acaccattcc gagcgtttcg aacattcaat ctcatgcgag
     1141 gatcatccga gagaagtcgg tcatccgctc tctgattcgc gtttgccacg aggtcgcctc
     1201 acggggctat ggcgactacg ggcccgtcga ggagttcctc gatcagtcgg agtcgtcgat
     1261 ttttgcagtt gcgcgagagc gcgctcgaaa tccgtacgag cacgtcaaag acgtcgtcat
     1321 gcggactttc aaggagatcc acgagacggc taaccgcggt gaggcgatca ccggtctgcc
     1381 tacgggctac aagcagctcg acaagatgac ggccggtatg catccaggcg acctcatcat
     1441 cgtcgctgga cgtcctggca tgggaaagac ctcttttgcg ctcaacgtcg cgcaccacgc
     1501 ctgtcaaaag agcaaggcgc cggtgggcgt gttttcgctc gagatgccga aagggcagct
     1561 ggttcgccgt cttcttggaa gcgaagcccg cgtcgacggc aaccgcattc ggacgggaca
     1621 gctccacaag gacgactggc cgaagctcgc tgatgcggct ggcagcctga gcgaaatgcc
     1681 gatttggatc gacgacacgc cggcgatctc gatgctcgag ctgcgggcca aggcgcgtcg
     1741 tctcaagtcc gaagttgggc tcagcttgat tgtcgtcgac tatttgcagc tgatgcgttc
     1801 ggggtcccgc aacgactcac gggagcaaga gatcagcgag atcagccgaa gcctgaaggc
     1861 gctcgccaag gagctcgaga ttccggtcat cgctctttca cagctcaacc gcggcgtgga
     1921 atcacgcggc gtcaaagaca agcgcccgca gctctccgac cttcgcgagt ccggtgcgat
     1981 cgagcaggac gccgatacca tctggttcat ctatcgcgac gaggtctaca accgcgagag
     2041 cgacgaccgt gggatcgccg agatcatcat cggcaagcag cgcgcaggcc ccacgggaac
     2101 ctgccgcgta cggttcttca acgagtacac ccggttcgat gacctcgcgg agtccgatta
     2161 ctatggcggc gcctcgtccg ggatcgccga tttccaagac aactgacctc acccccgaag
     2221 acggttttga cgtctcgggg ggctcggtca aagggctttg acctcagcga tgaggccttg
     2281 aagcaccttc ttggcgtcgc cgaagatcat catcgtgttg ggatactcga agagctcgtt
     2341 cttgatcccc gcatagccag ggctgaggct tctcttcacg acgaacaccg agcgtgcttc
     2401 atgggcgttc aagatcggca tccctgcgat ggggctggtc gggtccttgc tggcgatcgg
     2461 attcaccacg tcgttcgcac cgaggacgat caccacgtcc gtgttcttga actcggggtt
     2521 gatcgcgtcc atttcgagaa gctcttcgta agggacatcg gcttccgcca gaagcacgtt
     2581 catgtgaccg ggcatgcggc cggcgacagg atgaatcgcg taggtgacct tggtgccgcg
     2641 cgccttgagc atgtcggcga gctcgcgaac ggcgtgctgc gcctgtgcca ccgccaagcc
     2701 gtagcctggc acgatgatca ccgattcggc gttctcgaga atcatggccg cttcctcggg
     2761 gccgcaggag gtgacggtcg tgtactcgtc actaccaccg cccccgccgc cgtccgtgac
     2821 gccgaagccg cccaccagga cgttgagcag cgagcggttc atcgccacgc acatgatctg
     2881 tgtgaggata agccctgccg cgcccaccat cgcacccgcg atgatcagca aggggttgcc
     2941 gatgacgaac cctgccatcg aggccgccac gcccgagtac gagttgagga gtgagaccac
     3001 gaccggcatg tctgctccgc caatgggaat cgtcagcagg acgccgagga gcagcgagac
     3061 ggcaccgagg actagcaccc agaccaggga gtcggaagcg gtcgcggcca cggcgatgcc
     3121 accgaacgcg aaggaaccga agagcaacgc aaaggtgatg taatgtctcg ccgggagcag
     3181 gatcggttgg ccatttttca gggtgccctg cagcttgaag agggcgatga ggctacccgt
     3241 gaaggtaatg ccgccgatca ggatcgacag catcacggtg accgcctcag gggtgccaag
     3301 cgtttggtag acgcggctga ctggcgcctg ctccacgatc ttgtcccagt agacggaggt
     3361 cgcgaccagc gccgacgcga tgccgccgaa cccgttgaag agggcgacca tctcgggcat
     3421 cgaggtcatc tgaacgcgga tggcgaagat cgcgccgatg atcgtgccga gaaggatccc
     3481 tccggcgatc cagcggtagt cgacgaggcc gagctccaga agcgcaccga tgatcgcgag
     3541 cagcatgccc actgcggcga gcgcattgcc acgccgggca gtcttcacct tggaaagctg
     3601 cttgatcccg aaaatgaaaa ggaccgtgga gagaagatag atgatcggaa ccacagtcga
     3661 acgcatgatc tcgtggttca tttgctcacc gcctcccgtt ctccggcagt gacgcctttg
     3721 gaatcaggct cgagagcctt gggcttcttc ttctttccga acatgcgcag cattctgtcg
     3781 gtcacgagaa agccaccgac cacgttgatc gtcgcagtga cgatggcgat cgcgccgagg
     3841 atgttggcta cagtcggcgc acccgtcgtt gcggctgcca ccgcgccgac gaccgtgatg
     3901 ccactgatgg cattggcgcc gctcatcaag ggcgtgtgca aggttggtgg caccttggtg
     3961 atcatttcaa agccgacgaa gctggcgagc acaaaaacgt agatgcctat gagcagaggt
     4021 cccagggtca tggtcgctct cctttcttag agggcctcgg cggtcggtcc gtgccggact
     4081 tcgccgttgt gagtgagtag ggcgccatcg agaatctcgt cctcgagatt gaggaccact
     4141 tttccgtccg tcacggtgct gagcagcagg gccaggatgt tgcgagagta gagcaggctc
     4201 gcgtcggccg gtgtcgctgc aggtaggttt gggtaatcga tgatggtgac gccatgctcg
     4261 acgacgatct cgccgttcct cgagagctcg cagttgccgc cttgctcgac ggcgaggtcg
     4321 acgattacgg ctccttcgcg catctcctcg accatgtcct tggtgatgag cttgggcgcc
     4381 gggcggccgg ggacgagcgc tgtggtgatc acggcatccg cggccactac cttcttgcgg
     4441 acgatggctt gctgcgcctt gagttgctcc gccgtgagct cgcgcgcgta gccgccttct
     4501 ccttctccgc tctggtccat cggaaagtcg atgaagcgtc caccgagcga ttcgacttgc
     4561 tccttgaccg agggtcgtat gtccgaaacc tcgaccaccg cacccagacg gcgcgcagtg
     4621 gcaatcgctt gaagcccggc gacgccggca cccatgatga cgatcttggc tggctgaatc
     4681 gtcccggcgg cggtcatcag cagggggaag tacttatcga ggtaggtcgc tgccatgagc
     4741 acggcctggt aacccgagat cgaagcctgc gagctgagcg cgtccatcga ctgggctcgg
     4801 ctgatgcggg ggatgagctc catcgccacc gtgctcacct tgcgatctcg caggatcttc
     4861 accgactcga gatttcgatg tggggacatg aagccgatga gaagggcacc ttgcttgagt
     4921 ttggacgcct cttcaggcgt gatcgggtag acccgaaaca cgatgtccgc cgagccccat
     4981 gcagcggcgg cctcggctcc ggagacgatc ttcgccccgg catcgagata gtgctgatcg
     5041 gtgaagtggg cgtacttacc cgcttcgctt tctacgacga catcgaagcc ttctttaacg
     5101 tagcgcttcg ttgtttctgg tgtgcaggcg acccgagtct cgccattggc gatttccttg
     5161 ggcacgaaaa cctgaggcat gccttcctcc tgagcgagcg gtgtcggagc gacgaacccg
     5221 atcgaactag tcgacaatcg gatgcccgcg tccgggggtg ttagtggcga tccagggcga
     5281 tgtccagtgg cgagcaaaaa agccttcggc gcaccttttg cgtttagtcg cagcctgcta
     5341 gatccggccc gcgaacctgg aggacaagag catgggaaat cccgcgaagc tcgatgagct
     5401 cgctatcaag acgattcgtg gcctgagcat ggacgccgtc caggccgcga actcggggca
     5461 ccccggaacg ccgatggggt tggcgcctct gggatgggcg ctcttttccg agctccgccg
     5521 gcatgatcca gctcatccgg actggccgga ccgtgaccgc ttcgtgcttt cctgcggcca
     5581 cgcctcgatg ttgcagtacg cactgcttca tttgagcggt tacgcggtgt ccttggacga
     5641 tattcgcaag tttcggcagt gggggagcat cacccccgga cacccggagt cccatgtcac
     5701 ccctggagtc gaaacgacga ccggtcccct tggacagggc gttgggaatg gggtcgggat
     5761 ggcgatggcc gagcggcacc tggccgcgag gttcaaccgg ccggggcaca cagttttcga
     5821 tcatcgaacc tgggtcatcg catccgatgg ggacatcatg gagggcgtcg cctcggaggc
     5881 ggcctcgatc gcgggtcacc tcaagctcgg caagttgctg gtcttctggg atgacaaccg
     5941 gatcacgatc gacggcagga ccgagctctg cttcagcgaa gatgtgcttg cgcgtttcgc
     6001 atcgtacggt tggcacaccg agagtgtcga cgatggtgaa gacctcgccg ccttggtcac
     6061 cgccgcgcac gcggctgtcg ccgatgcgcg cccgagtttc atccgcgtgc gcaccatcat
     6121 cggtcgcccc tcacccaaca agcaggatac gtcggctgcg cacggcgccc ccctcggcga
     6181 agaagagatc atcgccacca agcagatgat ggagtggccg accgagccct ttttcgtgcc
     6241 cgacgagatc gtcaacgcga ccgaggccat tcgggaccgt ggggccaaga gctacggggc
     6301 ctggcagcgg cgcttcgaaa gctaccgggc cgagttcccg gaccaggcag ccgagctcga
     6361 gtcggtgatg gcgggggagc ttcctgcggg atgggatgag gatctccccg cgttcgaggc
     6421 cgatcccaag ggtattccaa cccgcaaggc aagtggtgcc gtgctgaacg cgctggccgc
     6481 gaagatcggc gggctcgtcg gcggttccgc cgatctggca ggctccaaca acagccacat
     6541 gaagggcctt ccaagcttct tgcccggcgc ggagggcatt ccccgcaacg tcgattgggg
     6601 cattcgtgag cacggcatgg cggcagctct gaatggaatg gcgctccatg gcggcgtcat
     6661 cccatacggc gcgactttcc tggtgttcag tgattacatg cggccctcag ttcggctcgc
     6721 ggcgctgatg aagctgcaca cgcgctacat ctacacgcac gacagtatcg gcctagggga
     6781 agacgggccg actcaccagc ccgtcgagca tctcgcggcg ttgcgtgcga ttcccaatct
     6841 cgcggtgttt cggccggccg acgcgaacga ggttcgcgag tgttggaaag ccgcgctcga
     6901 gtgggatggt cctgcggcgc tggcgctcac acggcagaac gtggcggtgc tcgatcggaa
     6961 gatccttgca agcgctgaga aggcaaagcg aggcgcctac acgctccgcg aggcaggggg
     7021 cggcggtcct gacgtgatcc tcgtagccac cggtagcgaa gtggagatcg cactcgctgc
     7081 ggcagacgtc ctcgagcaag aaggtgctgc cgtccgtgtc gtcagtatgc cgtgctggga
     7141 gctttttgcg cgacaagatg aacagtatca gcgagaggtg cttccgcgtg ctggtgccaa
     7201 ggtcgtcata gaggccggga ttcgccaagg ctgggatcga tgggtgggca acgaagccgc
     7261 cttcgtcacc atcgatcggt tcggcgcctc tgcgccgtac gaggtgctct acgaaaagta
     7321 cggcatcacc gcagagcatg tggtggcaga agcacagcgc ttgttgtctt agcgcgccgc
     7381 ggtgagtgca gcgtcgtagt cgggctcttt gacgatgtcg gagacgagct cggtgtgaac
     7441 gactttgtcg ttctcgtcca cgaccacaat cgcacgcgcg gcgaggtcct tcatggcccc
     7501 gtcgaccatg cggacgccat agtcgtcgag gaagctgcct cggaacgtgg aaagcggaat
     7561 cacaccctcc aggccttcgg ccgcgcagaa acgcttctgt gcaaacggca ggtcggagct
     7621 gatcaccagc acgacgacgt tcttggcgct cgctgcgcgc tcattgaaag ttcgcgccgt
     7681 cgcttggcac acgggcgtgt cgtagctcgg gttgatcgtg aggattttct tcttgcccgc
     7741 gaagtccttg agcctctttt cgctgaggtc ggatcccacg aggagaaagt ccggggcagt
     7801 gctgccaggg gcgggcaggt cgccgttggt ctgaaccggg gttcctttga gcgcagtggt
     7861 ggccatgatt ctccttcttg agcttgagcc gttccgagtt atagggactt ggccccaaaa
     7921 accaagcggt tgccatcgaa aaagaaattc attatcagta gtggatgaaa ccgtcgctcg
     7981 tcgatttgcc ctttgggcag cacgctgaga tcgtgtcgat cgactgcggg cgcagcctat
     8041 ctcggcgctt gatggagatg gggctgcttc ctggaacccg cgttcgcgtc gttcgcgtcg
     8101 cgccgcttgg cgacccgatc gagcttcggc tgcgaaacta ctccctttca ctgcgtcgag
     8161 ccgaggcagc caagatcgcc gttcgcgtcg aaggagcagc gtgagcgctg gtgcaccagc
     8221 ccggcaggtc ctgattgccg gcaaccccaa ctgcggaaag agcacgctgt tcaacgctct
     8281 ttcgggagga agtgcccacg ttgggaacta tccgggggtg accgtcgata ggaccagcgc
     8341 gcgcctgcga tcgggcgcgc tgaatatcga gttggtggac gtgccgggaa tgtacagcct
     8401 gaccgcaggc tcgccagaag agcagctagc ggtgaacgcg ctgatcgacg aaaagaccgc
     8461 gggggtgatt tgcgtggtcg atgcaacgac cttgcagcga ggtctatatc tggccttgca
     8521 gctgatcgag tccggagttc ccgtcgtaat cgcgctcaac atgatggatg ccgctgagcg
     8581 ccttggtctt cgcatcgata cggagcggct ggcggcgctc ctttgctctc cggtcatccc
     8641 ggtggtcgct acgagacgaa aagggctgga cgcgctcgtt gcagcgttag agaacgagct
     8701 ggcctcttcc gcggttccga ccgagctcgg aatcgagtac ccgcagacga cacaagcgga
     8761 tgtgaccaag ctcgttccag ccatcgccga atggcgcccg ggcacgccgc ccgagcagct
     8821 ccgggtgttt gcgctctggt gcctcctctc gctcggggat gatagccttc gtgacgtacc
     8881 cgattcggtg aggaaggccg tggagcggac ccgagcggta gcagagtccg agggtcgcaa
     8941 cttggatctc gaaatcatct cggcccgcta tgcctggatc gatcgcgcgc taaaggaatc
     9001 ctgctctgcg cacgatccga gtccgtcatg gagcgagcgc atcgaccgtg tgctgaccca
     9061 tccgctcgcc gggctcctga tcttcgcggt ggtgatgttg ggaatcttcg aggccctttt
     9121 tgccggagcc gagcccttga tagaggggat cgagagcctc acggggttca tgcaagcgca
     9181 ggccgcggcg ctcatcccgg ccggggtgtt tcgcgacctg gtctccgagg ggatcatcgc
     9241 cggggtcgga aacgtgattg ttttcgcgcc tcaaatcttg atgctctttc tcttcatcgg
     9301 gttcctcgag gactccggat acctcgctcg cgtggccttc gtcatcgaca gggtgatgaa
     9361 gggggtcggc cttcacggaa aggccttcgt tccgcttctg tcgggtttcg cttgcgcggt
     9421 gccggccgtc atggccaccc gtacgatcga acggcgaaga gaccgtctcg tgacgatgct
     9481 tgcacttccg ttgatgtcgt gcagcgcgcg cctaccgatt tacatcttgg tcgtgggcac
     9541 ggtcttcgcg ggacagtcgt tcggttggtt cagtgctggc gctgtagccc tgttctccat
     9601 gtataccctg tcggtcctcg tcacactcgc ggccgcggcc gtcattcgaa ggacggtgct
     9661 acccggtccg gcacccacgt tcgtgctcga aatgccgccg taccgttggc cctcggccca
     9721 gatcctctgg ctcaatgcct ggcgccggct gcgcatcttc ctggtcgatg cgggcacgat
     9781 catcctcgcg atgacgatcc tgctctgggc gctgctctcg ttccccaagt ccgatgatat
     9841 cgctcagcac tacgccgggg agcgccaacg gattctagag accgcggctg cagagccgaa
     9901 gcaagccgag gcgctacaat cgctcgattc caaggaagcc ggtgagcagc tcgcgttcag
     9961 ctttggcggc cgctttggtc acgcgatcga gccgctgctg aggccaattg gcttcgactg
    10021 gcagattggg gtcggaatca tcggcgcctt tgccgcccgc gaggtgttca tatccacact
    10081 gggcgtggtc ttcggaatcg gcgaggcgga cgaggagaac aagcccctcc gaacggccct
    10141 ccgggaggca cgccatgccg acgggactcc cgtcatgacc cctctcagcg gcgtttcgct
    10201 catggtgttc ttcctcctgg cttgccaatg catgagcacg atcgccgtag tgaggaagga
    10261 gtccggctcc tggaagtggc ccgtcttcct attctcctac atgacggtgc tcgcctacat
    10321 cgcctccttt ctggtgtatc agggtgggca gttaatgggt ttcggatagg ttttgggtat
    10381 gccctggcag caaatcgtga caggcttgat cgtcgcgtcc gccagcgcct acgtcatttg
    10441 gcggctccgc ggtaagaagc gaaatcaacg ccggtctcag ggtccagacg tgcctgtctc
    10501 gagattgacg aagaagcgtc cgccgtcaga ttcgtgcggg cattgatcgg aatgattaca
    10561 ttggatcgaa catgagccga tcggccacca ttgaaggact gcgaaagttc cgaacgaagc
    10621 tcctgcgcga gagagatccg tggcaggaca cctcgcgccg gtttcgcatt cgacgtttcg
    10681 cgggtcccga gtgctttctc gagcacctcg aaggggtccg ggtcgcgcat ctcacggatc
    10741 tgcacgtggg acgcgtcacg cccatggccg tgcaatacga cgcagtgtcc atgaccaacg
    10801 caggcaagcc cgacatcgtg gtcatcagcg gcgatttcgt gtgtcacagc caggtctacc
    10861 tggacgacct caccgacgta atcagcaaga tcgacgcccc ggtgtttgcc gtgctcggga
    10921 accatgatca ctgggcgggg gcagagcctg tgcggcaggc gctccggcgc ggcggcgccg
    10981 aggtgctcga caacgcgcac accagcatca ccatcggaca tcagccactc caggtcgtcg
    11041 ggctcgatga cgcgtatacc gggcatgccg atcgacgcga ggcgctcaag ggtctccgag
    11101 cggaccttcc cgtcatcgcg ctatcccaca tcgcagagga ggccgatgcg ctctgggagg
    11161 aaggcgtccc cctggtgttc tcgggccaca cccatgcagg gcaggtcacc gttgccaggc
    11221 ttcacgagct ctcggtaggc aagctagccg gtcacaaata cattcatggc ctctatggtt
    11281 cgagagacgc tgaggacaaa aaaggggcgg tctatgtagg tgccggggtc ggtgctgcga
    11341 tcgttcccct tcggctaggg gagcgcgcga aacgcgaagt ggcgttcttc gagcttggcg
    11401 cgcagcctgg ctctttcgac gaacaccaca gcgaacaagc ggcgcaccgg gggcgagtcc
    11461 catcgcctaa gaccaaggag cggcgcgctc aggcggtctt gcgcaaacaa gagcgtcgcg
    11521 aacgcaagaa cggcaatgga aacggcaacg gaaacggcaa aggtgcccgt ttcaaccctt
    11581 aggaattcga ccccaagaag tgctccgctt ctagagctgc catacaaccc gtaccggcgg
    11641 ccgtgatcgc ctggcgccac ttcttgtcct gcacgtcgcc ggcggcgaag acacccggga
    11701 tgttggtctg agtcgagtcg ggcttggtca cgatgtagcc ctgctcatcg gtgtcgatct
    11761 ggccgttcag gaaggcggtg ttcggggtgt ggccgatcgc gaagaagagc ccggcgacag
    11821 gaatgtctcg ctcctcgtcg gtcttgaggt tcttgacccg gatcgcttcg aggaagtcgg
    11881 cgcccttggc ctgcgtcacg acctgcgacc agagcatttg gatcttcgga ttgtcgagcg
    11941 cgcgctgctg catgatcttc gaagcgcgga gctcatcgcg ccggtgaatg atgtacacgg
    12001 tcgatccata cttcgtgagg aacgacgctt cctcgaccgc cgagtcgccg cctccgatca
    12061 cggcgagcgg ctgatttcgg aacgcgggca acgctccgtc gcagacagcg caggcggaga
    12121 ttcccttttg ccagagctct ccatcattgg cgccgggaat ctcgagacgc ttcgccgtcg
    12181 caccggttgc gatgatcacg caatcggcgc tgccctcctg ggcgtccgcc ttgaagcgga
    12241 acgggcggct ggagaagtcg atctcgttga tggtctcagt gtgaatcgtc gtgccaaatc
    12301 gctgcgactg cgcgcgaaac cggtcggtga gctccggacc catgatccct tcgggaaaac
    12361 ccgggaagtt ctcgacgtcc gtggtggtgg tcagctggcc acctgcggcg atgccaccgg
    12421 ccatgaaacc ttcgaaaagg acgggcttga gctcggcgcg agcggcgtaa atcgctgcgg
    12481 tatgggcggc aggtccgctg ccaatgataa ggacacgatg gctcatggca ctcctttaga
    12541 cgcccgggtt atgcatacct gcaggcggcg gcgcaagccc taagcgatca gcttggacac
    12601 gtgcgcaccg atcgaaagag ctgcggtgag ccctggagat tcgataccaa ccaggtgcac
    12661 gaggcccggt tgcgaagcgg gcatggtgat cacgaagtct gcgaagccgc cctccgggcc
    12721 gactagcttc gggcgaatcc cactatagtc cggtgtcaag tgctcgggcc ggatcgcggg
    12781 cagatagcgg gcgactgcct cggcaaagcg ttccgcccgg gattcgtcga cggagtagtc
    12841 gatcgtctcg acgaactcgg cgtcgggacc tgcgcgacgc gcgccagcga tgtccgcggt
    12901 gatgtgaacc cccagtccgc ctcccgaagg aagcggatag atcaagctat tgcgcgtcgt
    12961 aggagcaccg gcaccgagcg cgaaatacga gcccttgcat gggtacagcc gccagggacg
    13021 ctcagtttcg ggcaagcccg atcttgccgc gacgtccaga gccgcaaggc cggccgcgtt
    13081 gacgactcgt tttgcttcca gctccatttc gctaccggtc gtgtcggccg ttcggacgag
    13141 ccagccatct cgggttttca gaatctggtc tacctgcgtg tggtagacga tcatgccatc
    13201 cgcttccttg atttcgtgca cgaggctcgc gatcagctcg tgtacgtcga cgatgccgga
    13261 gctaggagag tgaagggcga gcgcgcaacg aagctccggc tcctgcgccg tcgcttccgc
    13321 tgcggtcatc aagcgaagct cggcggcgcc gatctcgctt gcgtggcggg cgatggcgcg
    13381 aagacgttgc tcgtcttcgg cgttcgtagc gacgatcaat ttgcccgtat gcttgtgcgg
    13441 cacgtcgtgc ttctcgcacc actggtacag gcgtcgatta ccttcgatgc agagtctcgt
    13501 cttcatcgag tccttcggat agtagatccc cgcgtgaacg acgccgctgt tgcggctcga
    13561 gatttctctg ccgggcccga cctcgcgttc gatcactacg acagagtcga ggcgctttga
    13621 gagctcatgg gcgcaagcga gaccaaccac gccagcgccg atcacgaccg catcgactgc
    13681 ttcggacatc acggattctc ctaaaaagcg agtggatcaa ggcgctgagc tggaatggtg
    13741 agcgcaagct gcggcatcgc aagctcgcgc tgcttccggt acgcctcgta gtgcgtgagc
    13801 accttgcgga catacctgtg cgtctgagtg aaagggattc gttccaacaa gacatcgacc
    13861 ggcgcatcag cggggctttc ttgaatccat cgccgtacgt tgtggggacc gccgttatac
    13921 gccgcgatcg cgagtggcaa acgaccatcg aagcgccgaa tcaaagatgt cagataccag
    13981 gccgcaaact cgaggttgag ctcggggtcg aggagatcag ccggagtgaa atcatcgtga
    14041 ccgagagcgt gggcaatgag ctgccccgta cgaggcatga tctgcatgag accgaccgcg
    14101 ccggcgtacg aaacgatgtg cttctggtac gcgctctcga ctcgcatgac tgcgagcaga
    14161 aggttcgggt ctaggccgta gcggttcgct gccgggaccg cgagccattc gtaggcgcgg
    14221 ggcagggtat cgatgaattc cggaccctgg aagccgcctg cggttcccat gtcaccaagg
    14281 gcgatggcca cctctgctag ggtggaacgc tcttcgttgg acagttcgag gcgcgcgact
    14341 cgtacatcgc tcgcgatcgg ctcttgcgtt cggctgtcgg cttgggcaac gcaggtgagt
    14401 cccgctctgg cgatcggctt tccaagcgca tgacgccagg tgaggtaggt ttcgaacaat
    14461 tcggccgttg cagcctcgcg ttctcccaca cgaagaaggt cggcagcgcg cgcgatccac
    14521 ggatagctct cccagtggag ctctccgatt ggttcgagct tcttggcgag cgcgcgattc
    14581 gaggttcggg gcgcgcgggg cacgtctatt tcttctggat tggaaagtcc ctcgaggctg
    14641 ccgcttcgct cgccatgctc gagcctctga agcccgttgg aggcccagag cgcgtagtag
    14701 aggtctccct gcgcttccgc tgtagacttc gcttgagtga agtagagtgc cgcctcgacc
    14761 aacctgccgg agcgttccaa agcgcgcgcc aaatggtaca tccctgccgc ctggtagctc
    14821 gctccgggac gcttcacgag gttttccagg agctcgatga tgtgcccttc gcttccggtt
    14881 cccactgctg ccatggctgc accgaaaagg acgggcgaag gcaccccagc tcgtttgtcc
    14941 agcgtttgca ggatcgaatc cgtgtcagct cgaagaccgg cggccgaggc cagcttcaac
    15001 atgtcgagga gacggactga aggaatcgac gcgttcgagc gcccggcgcg caagcccgtg
    15061 agctgctgct ggacgtattt cggatcgcga gcccgcgccg tctcggccat gccgctcgcc
    15121 cgactttcga cgttgcgctg cgcagcgagg ctctccattg gaaagagctg agcagtgcga
    15181 agataggttt ccgcggcctg ccaacgacct tcatcgcggg cgagtcgtcc cgctaggtag
    15241 tgcacctcgg cgtgctgctc tcccgtgagc gggctttcga ggagcgccgc gatctccacc
    15301 ttggcctgct ctagagggcc ggtgcctacc aggcggcgcg cgcgctcgac gcgctcgtcg
    15361 ccggtcatcg acggcgtctc gtagccgagc gtctcgagac ggttgagctc ggcctcagcc
    15421 cagcctgcga ggcggtttcc cggctgctcg acgcggaccg cgtgcatgac cgcgattgcc
    15481 tcctcgtagc gagaccgacg aatcaggctc tggacttgct cgaagatgag ctcgctgcgc
    15541 ttcggagctt cggggtagtc gtccaagaga tggctcatga gctgatccgc tccggggtcg
    15601 tccgcacgaa gaagcgacag cacttccccg tatcgggctt cgaacctcac ggattcgtgc
    15661 gggctctgct gagcttctgc aaagaaggcg gccgctcgag cgggctgatt gcggaggagc
    15721 tcgagtcgtg cgctctgcag cgcgattcgg tcgacgatcc gcgggagtcc ccggcgggcc
    15781 aggtcgagct gaagtcgagc ttcttcgtag cttgccgccg cgatctgatc gccggccgca
    15841 agatatgctt cgaccgcgcg ctccttcggc ggcttggctt cagcgatggc gaatgcatcg
    15901 aggtacggac cgcacatggg agcccgccac tgaacgaggc tcagcggtgg gtcactcgca
    15961 ttgttcccat cagccacgca catcggaacg tcgggtgatg cgtgagccga tcgcgcaagc
    16021 gatgcgaaca gcaccgccgc gaagaggaga gcgatgattc cgcgctgggt catgctccct
    16081 acaagatggc aaatttgtga ggtgagacgc aaagatcgag tggctggcac tccctcttta
    16141 tacgtgttcg gcccgcctgc gcgtcattct tgcaagtgcg tgtcggattc taggtcctcg
    16201 ctggtcgcgc tgctgctctg ttcctcggca tcaggctcat ccgcgagctc gggaaggagg
    16261 acggtgacta cgccgacccc ggccagcacc gcaaagccca gtgccagccc gagggtgcgc
    16321 cactttgccg tctggtagac cgcgtcgcct ctggacatag gctcaggccc caagctcggc
    16381 caaggcggcc tttgctgcct cgacggcgtt tttcacgggc acgtccgttg actcgctttc
    16441 gatgcgcttc ttgagctcca cggtcccctg ctctagcccg cgcgcaccga ccgtaatccg
    16501 aacgggaatc cccaggaggt cggcatcctt gaacttgacg cccggtcgct ccgggcggtc
    16561 atcgaggaga gcttcgacgc cgtctgctcg aagcgcctcg tagatctcgg tcgcggccgc
    16621 aaggggttcg gcgtccttgc caatgggggt gatgatcacc tggtaggggg ctacttggac
    16681 tggccagcgg attccgtgct cgtcgtggtg ctgttccatg atggcagcga ccaagcgact
    16741 gacgccgatg ccgtagcaac ccatgacgat cgacctattc gttcccgctt cgtctaggaa
    16801 ggatgcgttc atcttgtcgc tgtagtgcgt gccgaggacg aagatatgac ccccttcgat
    16861 cccccggtag agctcgagcc tgccgtcgca gttggggcaa gggtccccgg aggtggcttg
    16921 ccgaatcgga gcgacctcgg cctcgaagtc cctgccgtgg ttcacgttcc tgtagtggta
    16981 cggggtctcg ccagcgcccg cgatcgcgtt cctcacagca accgcatccg ggtcggcgat
    17041 gatgcggcct ttgaacccga ccggacccac gaaaccgatc tccgtccgag tcgctgcctt
    17101 cacggctgcg tcggacgcca gcacgacctc gtcgacaccg agtagccgcg cgagcccgat
    17161 ctcgttgatc tcgtggtcac cgcgaacgat ggcgagaatg acttcgtcgc cggcgacgta
    17221 gagcaaagac ttcaaggtct tggatgccgt ggtgtcgcca ccaaagaagg ctacgacctc
    17281 gtcaatcgta cgcgtatcgg gcgtctcgac cttttcgagc tcggctaggt ccttccccac
    17341 cgacgcgctc gcagaaaact gactcgagcc ggccgttgca acctctacgt tggccgcgta
    17401 gtcgcacgca tcgcacgcga cgatccagtc ttccccggtc tgcgccagaa cttgaaactc
    17461 ggcgctttgt ttgccgccga tcgctccgct atcggcttcg acgacgcggt attcgagtcc
    17521 gagccgagta aagatccgat ggtatgtatc gcgcatctgc gcgtagcttt cgagcgcggc
    17581 ctcttcggac acgtcgaatg aatacgcgtc cttcatgatg aactcgcggc agcgcaagag
    17641 ccccgcccgc ggccgtggct cgtcgcggta cttcagctgg atttggtaca agttcagtgg
    17701 aagctgtcgg tagctcttga tctcccgccg cacgaggtcg gtgatgatct cctcgtgcgt
    17761 aggcgccaga tgatactcgc cacccttgcg atccttgatc cgaaggagtg tctggccata
    17821 gagatcccaa cgtccggtct ctttgaagta ctcggcggga acaaacgtag gcatgagaag
    17881 ctcctgggct cctgctgcgt ccatctcttc gcggatgatc tgctcgatct tgtgaaggac
    17941 ccgcgttccc aacggcaaga gctcgtagat gccggccccg accatccggg cgtacccggc
    18001 ccgaagcatg agcacgtgcg acggcatcgt cgcgtccttg gggacttctt tgacggtcgg
    18061 aatgaacgcg cgcgagtatc gcatcgcgcc gtcgtagcag gcagggagga gagtttccac
    18121 cggggtcgag ggaccccggt gtccctcttt ggaggtcaga ctccgatgat gaagctgacg
    18181 ctggtcgtgc ccgagccacc gatgttgagc gtctggacgt ttttcgcgcc ctcgacctga
    18241 tagtcgcctg cttctccgct gacctgcttg tggcaatcga gcacctggcg gacgccagtg
    18301 gcgcccaccg ggtggccggc tccgatgagg ccgccgctcg ggttgatcgg cgtcttgcca
    18361 ccgaaggcga tgtcaccggc ttcgatcgcc ttccagctct cgccgggtgc ggtgaggccg
    18421 aagtggtcga tcgccatgta ctcggaggtg gtgaagcagt cgtgggtttc gatcgcatcg
    18481 ataccgtcgc tgtttgcgat tcccgcgcgc ttctgcgcat cggtcaccgt gctccggacg
    18541 tgcggcaaga cgtacttggc gttgcggctc tcttcgatct tgtcctcgaa ttgaatccgc
    18601 gcggtgtggt gcccccatcc cttgatgcgg ggaatcgact cgagtggcac gccgacccgg
    18661 tcggcccact tcttggcgta ctctgctgag gcgaccatga tcgcggccgc gccgtccgtg
    18721 acctgagagc agtccgagat gtggatgcgg cccccgatgc tgccgttgaa ctcgtcccgg
    18781 ctcatcgcgt ggtcgtagtt catgtaccag gtgcgggtct gcgcgtttgg attgagcttg
    18841 gcattcgcat agttgatcgt gctgatctct gcgaggtgct cgtccttgag tccgtagcgc
    18901 ttgtcgtact cgtcacctag cttgccgaaa agcttgggga aagggaactc gattcccttg
    18961 gcctccagct cgtaccaagc ggcggtgccg aggaagtcac caccttcgcc cgggccgacc
    19021 gtcttcatct gctcgacgcc gaccacacat gcgagatcgt atcgcttcgc ctcaatctca
    19081 gccgaggcgg cgaggagggc gatgctgccc gatgcgcaag ccgcttcgtg acgtgccgta
    19141 ggaatgccgc tgaacgccgg gtcgatctcc aagaagaacg caccgaggtg gccctgcttg
    19201 ctgtagagtt cggcggcgaa gttgccggtg tgtgccactt cgacttcgct cggctcaatc
    19261 tgagtctcct cgagcgcgcc gagtaccgct tcgcgcatca tggcgacgaa gtgcttcttc
    19321 tctttgctcc agttacgagc aaagtccgtc tgatagccgc cgagaacgta aacttctgga
    19381 acctgtgcca tttctttcac caaaaccttt cgctcatgca gcgacgaaga acttacccac
    19441 attgacctga gggtcatcga agaacgttgt gccctcttgg gcgtttgcca gtgcggctgg
    19501 caccgcgagc ccagcctttt cgatcatctc aatggtcggt ttgaggccga tggtgtcgac
    19561 caggacgctt ggaggcgccc agttgaagcc gtagcccatg attcgatcga tcccggaaat
    19621 gtcgtccgtt acctctccga ctcggttgaa cgagtagctg atgtatccag cgatcacctt
    19681 ttgcgcgatc gccgcatatg gtccggttgc cgcagcgaag gcgcgcatcc cgtccacgta
    19741 tcgtcccacg cgatgaagat tcgaaatctc gtcgatgaag ccgaggtccg gtcgggagat
    19801 cgaggacttc gagacgtagt tctgagtctt cgggtcgagc acgatacgcc cgcctgcgtc
    19861 gtccttgcca aagaagcctc cgccggtctt gcggccgagg acgcccttgg ccatgagctc
    19921 ggccatgtac ttcggaagct tcagcgtttc atgcgcttcg tctggagcga gctcgtagat
    19981 gttgtcgacg atcgcgcggt ggatgtccca gccgaccaga tcgatggttg ccagtggcgt
    20041 catcgctcgg ccggtgtagg ggcccacgag ctcgtcgacc aagagcggtc cgtgctgctc
    20101 ggcgaggatc gcgcattcgt tgagcacctt gaagccgacc cgattgccag caaagccagc
    20161 agtgtcggcc gttcgcacca ttgcgcgtcc gaggagcttc gtgcagtact cgtcgaggaa
    20221 gtcgaccagc ttggggtccg tgtctttgcc cgcgatcagc tcggtcccga cgatcacgtt
    20281 gggtgggttg aagaagtgca ggccgacgaa gttctttcgg aacgaatcgc tgcggccttc
    20341 agccagcaga ttgatcgaga gaccggaagt gaccgttgcg acaatcgagt ccggacgtcg
    20401 caccttgtcg atgcgatcaa agaactcctt cttgagcggg agctgctcgg ttacggcttc
    20461 gaagatcagg tcggcttccg caaccacgcg atcgaagtct gcatcgtagc ttccggtgtc
    20521 gactcgcttg gccaccgtgc tcgagcggac catcttcacc gcagcagcga gcccgccatc
    20581 tgccttttcc ttggtgcgcg ccaaaaaggt gacccgcggt accaccgtcg tgaacagagc
    20641 accgcttccg aggcccatcg cgccgttcgc accgaggacc accacgtgtt tgattgagcg
    20701 ggagctcagc aaatccgagg acttgccacc tgccgctgat ggggtcgccg acaacatctc
    20761 ctgtcctttc tgtgcctcgg ctgtttacaa cttaactagc cgggaagccc caatgtgcgt
    20821 ggggtgaggg gtcgcgtcaa ccgaagtgac ccaaaaacag gcatttacag acgatttaca
    20881 aaacggcaat tttgaagatt tcggccaaat agccgggatt acggcgatct cgtacgcggc
    20941 gtgttaggga agcgtttcga tggaggcagc gtcgatcgcc aatgcgctcg ggatctcgca
    21001 agaggctgcc gagatcttcc tcagctcgga cgcgatcgac ctccacatcg agtcgttcct
    21061 ctggcgccga atcttcggct acgacctcaa tcgcgttcac aggggcgggc tcttcggccg
    21121 tcggttcttt ggtcacgcgg atcttccaag cgtccagcag gtagggctcg gcggcgccac
    21181 ctgggtgatc tcgaccaatc cgctgcgagc tgctgggcgc cgccggcagg tgttcgagca
    21241 gaacctcgct tcccttcgga acttgctcga tgaggcgcct ggggtgcggc acgttcggaa
    21301 cgccgcggag ttccgcgcgg cgcgagcctc tggcgagcat gcggccttca tcggcattca
    21361 aggtggcaac gccctcgacc atgatctcga cgcgctcgac gtgctcgccg accagtccgt
    21421 gcttcgggtg acgctcctcc acctcacctc gtcgcgacta ggtgccacga gtgcgcccgg
    21481 aggggggcga agcgcaggcc tgaccgattt tggcaaggcg tacgtcgagc gtttgaacga
    21541 gcttcggatc ggcgtcgatc ttgcccacat cagccgcccg gggtttttcg atgcggtgga
    21601 ggtacacgat aagacacagc cacttctcgt gacgcacact ggcctggccg gcgtctatga
    21661 gcactggaga aacatcagcg acctgcagct tcgcgcggta gccgactccg gtggaaccgt
    21721 tggtgtgatg atgcacgctg cctacctcgg tcgaggtcgc gtcaccgtcg ccacgctggt
    21781 cgaccatctc cagcacatcg tcgctaccgt aggagaagac tacgcgtcga ttggtacgga
    21841 ctacgacgga ttcatcattc ctccgcgcga tttgcctgga atttgggcgc tgccgcggct
    21901 ggttcaagaa atgctcgacc gaggctggtc ggagctgcgg attgggaaga ttctcggcgg
    21961 caatgcgttg agggtggtcg aggcattgcg cggctagccg cgcgcgcaca ggcttcgctc
    22021 atggcactgc tagaaaagat cgacgccgac ctcaagacgg ccatgctcgc tcgggacgaa
    22081 gtgacccgcg atacgcttcg catgatcaag tccgagctcc tgacactcga aaatcccgat
    22141 gagctcgccg ttctgtcccg cgcggtgaag tcacgacgtg agagcatcaa gagctacgtc
    22201 gaagggggcc ggcaggatct cgcggacaag gagcaagcgg agatcgaagt gatcgaacgc
    22261 tatcttccaa aacagctcag cgaagatgag gcccgcgagg cgatagccaa gatcatcgag
    22321 gagctcgggc tctctagtaa gaaggatctc ggacaggtca tgcgggagat caaggcgcgc
    22381 tatcccggcc aggttgatgg caaattggcc tcttcgatcg ccgcaaagct gctctcatga
    22441 agcgctcagt cttgcactga gctctgcgca gcccaccaaa gtcggtatcg ttcgcggatc
    22501 gccttgcgat cctggctcgc catgtctgtc ttgaagccgt agtcgtgacc ggaagcgcga
    22561 acgacgtctc ggcttgcaat ggcgcggacc tcggcgtccg ggtgatcgag accgtcgatc
    22621 agccactcga cgcgcgaccg ctcacgattg gccaggagcc aggcatgcca atccgctgcc
    22681 cggtccccga aatcctgcgc ggtcagcatg cgaagcacgc gccatgcagc ctctccgatg
    22741 acgctacgct cggaaagcac ggggatcagt gcctcgacgc aggcctcgtc gcgcagcacg
    22801 gcgagcgctc gaatggcgag caagcgcgaa cgttgatcgc tgtctgaggc gaccgcgagc
    22861 gctctgaggg actccttgag gccttcgatg ccttcttgtt cgtggaaaag ctcgagcgca
    22921 tagagggcgc tccgagatac gccttggtcg tcgtcgaaca gtgcagtcgc cagcggctct
    22981 agcagacgcg gatgcggaag ctccgctgcg acgagcgacg catagtaacg cgtttctggg
    23041 tcgtcggacc tcagaagctc tccgacgaag ggcacggctg gttcgccgaa cgccacgagc
    23101 gttctgcaca gtccggacac gctgctccct gccggaggct gtacgtgcgg ttcccaacga
    23161 ttgaaccaga gtcgcccggg aaaaaagtct ctgagcaccg gcaaaacatc ttcgcccatc
    23221 gcgagcaagg tcccatggtc gggagcttcc tcgttgggtc cgtgcatccc gagacgaacg
    23281 accatggtcc gcacccgcgc gaggaaatcc ggaggctcta tcgtgggcag cggggctcgc
    23341 ggtgcgctgc ccaccgcgct gaggtcaccg acgttcaggt gctcgaccag aaacttcatc
    23401 gccatcatgc gggcgtcgtc tcccctcgct ggcggaatcg acgtccagcg gcggaatgcc
    23461 tttccgagct cgcggtcagg ccaccacttg gcctctaagc tctcaccagg tggcggattc
    23521 gaggttcgcc agcggccatc cggaggctct gtctgcgggg cttcgctggg actcggagcc
    23581 tcgatgcgat ccgagggcgc gttcttgatc agataggtcg aatccgactt gttccctgac
    23641 cccatgggct atcagggtac accaatccgc atcaaaccag cattttgatt cggcgctctt
    23701 cttgagcgct gatggcctta ccgctcgccg cttcgcgcag cagttttttg atgcggagcc
    23761 caagaacgtc gatctcggtg gaggcgaagc tctcggcgcc aaagcgctgc acctgctcgg
    23821 tttggcgccg gaggatctcc atgtcttgcc gcaggatcct gagcgcgacc ggcttgaaca
    23881 ctgttctcag gatgagattc gggacgggcg ctttggccgc taccgtggcg aacatgtcgg
    23941 tgaccgtcgg actgcgcggc gtcagtgcgg ttgtggcgat gatgtgactg tgctcgctca
    24001 ggcggtattc gacctgtgcg atacagggga gcaggaatcg gtcccaatga aatacctcgc
    24061 ccccttgcgg ggcaaggatg cggccggcca agccttcggg acgttgctcg ccgatgaact
    24121 gcgcttctac gccaccctcc agcgcgcgga ttaccacctc gatgggtttt cgatccttgt
    24181 tctttcggaa gaggccggca tggacgaacg cagtgtgcgg aacatccagt gcgttttccg
    24241 ccacggcgtc gagaggaccc ggcatttcga gccgctcgcg aatggtgagg tatccaggct
    24301 cgtccgcgca aggtatcctg tacggcgcag acggcggttc gacatcggcc tcggagtaga
    24361 cccacaccca gccctgcgaa tcacgacaag cgaacgctgg aacacgacga gcggggtgat
    24421 ccggcgaacc gcaaaggccg ggcaccgctc tgcagacacc tgctccgtcg aactcccagc
    24481 cgtggtagtg gcattgaatg ttgtcgccag cgacacggcc agcagagagc ggcgcgttgc
    24541 ggtgcgagca acgatcgagc agggcgatgg tttgtccggc tgagtcacga aatgtaacca
    24601 gcggtaaccc gttcaatgaa accgctttgg gcttcgctcc gacgtccccg ctcgctgcaa
    24661 cgatgtacca gcccggcgca aaaccgttgt tcacgctctt ctcggtcaaa tgttgcagag
    24721 gttcgttcat catgacccgc gcgcatattc tgcgcccgcg tggctgtgac tttgtttcct
    24781 ttgcgtaatt agcacaaccc ggccgcaagt ttcgatggaa cggggcggtt tcgactgcta
    24841 cacgaaccgc atgtcacggc aaatcaacaa ggaacgtaca cggcgtcgcc tccttcaggc
    24901 gaccctcaaa acgatccagc ggtacggcca cggcaccttg acgaccggac gcgtcgcgca
    24961 gctcgcggga gtggcgcagc ccacgttcta tgtgcatttc cgaagcatgg accaagcgct
    25021 cgagcaggtg gccgaatggg tcgccgagga gctcagtccc ggtctcgagc cggaggcgtc
    25081 ggccgagctc gatcgggccg ccgacgtgct cgagcaggct gtgcggaagt gcacgcgctc
    25141 tctggtgcga gatcgaaagc ttgccgaggt cttcctcagc aatcgccggg accaaacctc
    25201 cgcgttgggc cgccgctgga ctgtcctcac cggaagcttg cgagagcgga tgcggcaaat
    25261 cgtagtgcgg gttcgtcccg acgtcacgcc agccgacgct gcgctccacg cagagctcct
    25321 cgtgagcgtc attttcggac tggcagaagc tcatctcgac ggccgcgtcg acgacctcga
    25381 ccgggcgacc gccacggcct cgcgggcgat cgtcacgagc atcttgacga gctcctcggt
    25441 cgccgacgct gcatgagtgg gctggtcggg ccggtccgct tccgatccat ctgcttgcgt
    25501 gttaagcccg tgatgtgact gacctgttca atccatcggc tgagcatcgt gccctccgcg
    25561 agctcgttcg ttcgttcacc gaaagagaag tcgacccgca ggccgagcag tcggaccacg
    25621 aggagcgctt caacgtagag ttgttccgca agctcggtga cttggggctt cttggcgtta
    25681 ccgtcgagga gcgatttggc ggcgctggtc tagatcccgt ggcggcggtg atcgcgcacg
    25741 aagagctcgc cgccgctgat ccgggcttca ccttggcgta cctcgcccac tccatgctct
    25801 ttgccaacaa cctacaggtc aacgggagcg acgcgcagcg aagcaagtac ctgcccgatg
    25861 cctgctcggg caagaaggtt tgcggcatgt gtatgagcga gccgggtgct ggcaccgacg
    25921 tacttggcat gcagaccagt gcggccaagc acggaggcgt ttacctgctc aatggcgcca
    25981 aaatgtggat cacaaacgga gcgatcagcg acacggagct cggcgatacg ttcctcgtct
    26041 acgctcgcat ccacggcagc ccgcgcaacc agctctcgat gttcatcgtc gaaaagggtt
    26101 ttccgggatt tagcctcggc cagaagatca agggcaagct cggaatgcgc gcgtcgacca
    26161 cggctgagct cgtgttcgag aattgcgaag tcccagccga gaacctcgtc ggctccgagg
    26221 gagcggcaac gctgcacatg atgcgcaacc tcgagatcga gcgggtgact ctggccggca
    26281 tgagtctcgg aattgcgcgg agatccatcg aagtcatgaa tcgctatgcg agtgagcgct
    26341 cgtcgtttgg caagccgctg agggacttcg gccagattca gcgctacatc gcccaatcgt
    26401 atgccgagta catggcaggt cgtgcgtaca cgtactacac cgccgcgacg atgagcctcg
    26461 agacgccagg ccaccgcctc gactccgacg gggtgaagct gttcacctcg acgatgggca
    26521 aggatgtcgc ggaccgggcg atccaagtgc tgggcggcta tggctacgtc tccgagtacg
    26581 ccgtggagcg cttgtggcgc gacgccaagc tcctcgagat tggtggaggc acgctcgaag
    26641 cgcaccagaa gaacatgtgc cgcgagctca gccataccga gcgcatcttg tgagaaaccc
    26701 cacaggtttc cgatcccgtt tggattttcc catcacgaag taggcgctcg cccattcgag
    26761 tcgctatgct gtgcggagcg acactttcct ttgggggaga gccgggcacc gggcgaaatg
    26821 cgcggtaggc gtcggctggg aaagtgtctg gggacgacgt gacacaggat tccgcagcca
    26881 gccagttgcc cagaccgcca gtcgatcttc cggtcctcgg aaagttcgcc ggctatgacg
    26941 ttcttggaag gctcgcgcta ggcggcatgg cggagatcct tcttgcgcgt cacgccaaca
    27001 ccgagggtga tcatcgattc ctggtggtga agaggatcct tccccatttc gagcaggaca
    27061 gtgacttcgt tcagatgttt ctcgacgagg cgcgtatcgg gatgcagctc aagcacccga
    27121 acatctgcca gttccatcaa ttcggtgcgg acgaagggtc gcacttcatc gtgatggaat
    27181 gggtcaacgg cgtgcccctg ggaaggctga ttcgcaaagc gcgcaactct ggtggtgtag
    27241 ggctgcctat cgcaattcgc atcggcgtcg acgtagcacg ggcgctgcac tacgcgcaca
    27301 tcgcaaatga cgaacacggc gagccgttca acatcatcca tcgcgacgta tcacctcaca
    27361 acatcatgat tgcctacacg ggcgcggtga agctcctcga cttcgggatc gcaaaggcag
    27421 atcatagggc tcacaagact caagccggcg tggtcaaggg caagtttgcc tacatggcac
    27481 ccgagcagtg cactgctggt caggccgacc accggctcga catcttcgcg cttggcgtct
    27541 gtctcttcga gacactcacg gggcgctcgt tgtaccggcg tcaaaccgaa gccgcgacga
    27601 tgcgagccgt gatcatggat ccggtcccct cgctcaagga gcgtcttccc gacgtgtccg
    27661 acgagctcga caagattctg cagaaggcgc tcgccaagga ggcaagcgat cgctacgtga
    27721 ccgcggaggc ttttgcccgc gatctggagg agttcggcaa agcaaacaag ctcttcgcga
    27781 cgcagcgtga ggtcgccgag ttcgtcaaga aggtgtttcc cgaggagttc acgcggggtc
    27841 cgcgcgtcga tacggtcccg ttcggagcat cgatcacgct cgacatgcat catgccaacg
    27901 actcgtcctc tgcgaagtct tacgtatcgc agatccctgg cgcgattcgc gagctcgagg
    27961 tcgatgttgg aggctacggc gacctcggac acttgctcga ttcgccggtc gtcgcgccat
    28021 tgccgctgcc cgcgtccgaa ccggcaaact ccgatgcgca ggctgctcca aaagaggttc
    28081 caaagcgcgc cgcgccgaag aagaggcccg cggggcccaa gaagtcatca ggcttcggct
    28141 ggaagggtat cctgatcact ctgctcgtgc tgctcggcgg agtcgcggtt gcttatgacc
    28201 agagcgacat cgtgaaggat gccgtcggta atctgagcga gatgttcgag gagaaggggc
    28261 cgcccgaggt gtcggtagac gacaacccgc tactacgaga gcatggggcc cgcattct
//
LOCUS       DASRZF010000205         5732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3927902, whole genome shotgun sequence.
ACCESSION   DASRZF010000205 DASRZF010000000
VERSION     DASRZF010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 5732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5732
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3927902"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VFG22_09190"
     CDS             <1..383
                     /locus_tag="VFG22_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416457.1"
                     /translation="QSLVEIMLPRTEAIPLDPAPMDLPRAFDGFLADEPEHNVKDLEK
                     ALVLIEFGPLAFDKKLTTFSRLDPEARSVHWNQWAVSDDLLRRQVSIAMRKFFNLVYF
                     DRAEVWPYIGYLGPSMKRAGKAKP"
     gene            380..1909
                     /locus_tag="VFG22_09195"
     CDS             380..1909
                     /locus_tag="VFG22_09195"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HET6416458.1"
                     /translation="MSGGIIDLSKEAPPRELSTEICIIGSGCGGATAARLLSESGHEV
                     IILEEGRDLVGPERTQRDAAMYDQLYMDRGGRATSDLSVSVLQGRVLGGGGVINACDV
                     VPSPDAVLQHWATKYGLEDFAPASFKPFQDAALEDLSASRIPETLVNKANNKLRQGAE
                     ALGLRGEVMLHNRVGCQGLGTCLIGCPIHAKRNPRMVALPAAIEAGTRVFTRARATQI
                     KKADQEMKEVTVRTLDPLGYHERASLIVRAKIVIVAANAIASAQLLLRSGIGNEHVGR
                     NLTLQPQLPIIGLFDERIAAFDGVPQSYAITEFEEDDNAEYGLWGYRIEGISGTPGIT
                     ATLLPYSGAEGMEGMALYDRMASSLILVPDAPSGRVEVARDGRMTIEYTQQTNHQERL
                     RHAVETGAKAYLEAGAREVLVPTAPPLQIRSSSDAKKASQLTFKPATAPLISAHQQGT
                     VRFAASERDGGANLDGEVYGTRGVYVFDSSGYPTSASSHTMTPIIASSRMLTTKLLTR
                     I"
     gene            2027..2284
                     /locus_tag="VFG22_09200"
     CDS             2027..2284
                     /locus_tag="VFG22_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416459.1"
                     /translation="MMVPEEIDLNELRRQLAARFGDAPPAGYVCGKGDLRAAVVDILG
                     CSELEAEQLVDTLESRHLIRYEGDRSDGIDRLETHWLLGND"
     gene            2317..4413
                     /locus_tag="VFG22_09205"
     CDS             2317..4413
                     /locus_tag="VFG22_09205"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HET6416460.1"
                     /translation="MKRFALAFTLISLVAGCTSSDECADLGSTCPALEQRVEQLLSEM
                     TLEEKVAQMSGTTFISGLYGTEPWNVPGVERLGIPPFKMSDGPRGVGVQDGSTAFPVA
                     IARGASWDPELERRVGEAQGRELRAIGGNVLLAPTINNLRHPSWGRSQETYGEDVHLL
                     SRFAVASVQGLQKYVLANPKHFAANSIERTRFQVDVSLDERTLREIYLPHFRAAVHEG
                     GAASIMSAYNSVNGQFCGENEVLLRGILKGDWGFDGFVLSDFTLGTHPDSAVNGLDLE
                     MPTANIFRSLLQQVRSGEVPEEVVDDAVRRMVRKKLQYKLDKPSAVDESVIASDEHLA
                     LAREAAVRGSVLLKNDSNALPLDRSVLTRIAVVGELADAINIGDNGSSSVRPAFVVTP
                     LDGIRAAVGDDVSVDFIGTDTLNGNDLATLEAADAVIVVTGLTLDDEGEGLIGAGDRI
                     DLGLSPERVALIQDVAAANARTIVVLEGGGAITMGDWMPDIEALLMVWYPGQMGGHAI
                     ADLLFGIENPSGKLPITFPVSLDQLPEFDNESLAVTYGYFHGYRYVDLNGTDPEFPFG
                     FGLSYTTFSVANLAASQAQASAGDVVRFSVDVTNDGPVAGSEVVQLYVTYPNSSVERS
                     VRELKGFAKVELEPGATETVTIELRVNDLAYYDVAASAWALEALEHEVLIGTSSRDLP
                     LATNLSVNAAAPVEVY"
     gene            4462..5247
                     /locus_tag="VFG22_09210"
     CDS             4462..5247
                     /locus_tag="VFG22_09210"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6416461.1"
                     /translation="MQRFVQIAAASVLVVGATVGVSSAAEIGFRSVDFPTTGGIQGRA
                     DVYESQDSSATLILLFHQAGWSRGEYREIAPQLVERGYRVMAVDQRSGGGVNGVPNET
                     HRRAAKKGLGTSYLDAYADLEAALAYAHEALKAERVIVWGSSYSASLVFRLAAEHARD
                     VAAVMAFSPGEYFQKGVGAEYVRGFAKRVDQPLFVTSAKTEREQAKPIFDASPATKKI
                     LFTPASMGQHGSRALWKKWKDQDVYWAAVGAFLKEYAPPTPPK"
     gene            5294..>5732
                     /locus_tag="VFG22_09215"
     CDS             5294..>5732
                     /locus_tag="VFG22_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416462.1"
                     /translation="MTQLPVQRVMDTERARLAGRQLGVDVDIHSQLRPFEAHRWREGD
                     SPHPTLHLDDVSAIPFLADIAGIEEYQHRGRLRAGDGDIYVTITPAGEGYESYCRHTL
                     RMGHPEHLVADPLGPAIAVARSCKVGAAHARLVEATRESGGLTI"
BASE COUNT         1118 a         1779 c         1789 g         1046 t
ORIGIN      
        1 tgcaaagtct ggtcgagatc atgctgccgc gaaccgaggc gatcccgctc gatcccgccc
       61 cgatggatct tccgcgcgcg ttcgatggtt tcctcgccga cgaaccggag cacaacgtga
      121 aggatctcga aaaggccctg gtcctgatcg agtttggtcc gctcgcgttc gacaagaaac
      181 tgaccacgtt ttcgaggctc gatcccgaag ctcgcagcgt gcattggaac cagtgggcgg
      241 tcagcgacga tttgctgcgg cggcaagtca gcattgccat gcgaaagttc ttcaacctcg
      301 tctacttcga tcgggccgag gtctggccgt acatcgggta tcttggtccg tcgatgaagc
      361 gcgccgggaa ggcgaagcca tgagcggcgg gatcatcgac ctttccaagg aagccccgcc
      421 gagggagctc agcaccgaaa tctgcatcat cgggtcggga tgtggagggg caacagcggc
      481 ccgacttctg agcgagtcag ggcacgaggt catcatcctc gaggagggac gcgacctcgt
      541 cggacccgaa cggactcagc gcgatgcggc gatgtacgac cagctctaca tggaccgagg
      601 cggtcgtgcg accagcgatc tttccgtgag tgtgctgcaa ggacgtgtac tcggcggagg
      661 cggagtgatc aacgcctgcg acgtcgtccc ttcgcccgat gcggtgcttc agcactgggc
      721 gacgaagtac gggctcgagg acttcgcacc cgcgagcttc aagccctttc aagatgctgc
      781 gctcgaagac ctctccgcaa gccgcattcc cgagactctg gtcaataagg ccaacaacaa
      841 gcttcggcag ggcgccgaag cgctcggcct ccgtggcgaa gtgatgttgc acaatcgtgt
      901 gggctgtcag ggacttggca cttgcctcat cgggtgccca attcatgcga agcgaaaccc
      961 tcgcatggta gcgctccctg cggcgatcga agcgggcacg cgggtcttca ccagagcccg
     1021 ggctacgcag atcaagaagg ctgatcagga gatgaaagag gtgaccgtgc gcaccttgga
     1081 ccctctcgga tatcacgaac gagcttcgct catcgttcga gccaagatcg tcatcgtcgc
     1141 agccaatgcc attgcctcgg cgcagctgct gcttcgctcg ggaattggaa acgagcacgt
     1201 cggccggaac ctcacccttc agccgcagct tccgatcata ggcctattcg atgagcggat
     1261 cgccgccttt gatggtgttc ctcagtccta cgcgatcacc gagttcgaag aagacgacaa
     1321 cgccgagtac ggcctctggg gctaccgtat cgagggcatc tccggcactc cgggcatcac
     1381 cgcgaccctt ctgccctact ccggcgctga aggcatggaa ggaatggccc tctacgatcg
     1441 catggcctcc tccctgatat tggtccccga tgcgccctcc ggccgagtgg aggtcgctcg
     1501 ggatggcagg atgacgattg aatacacgca gcagaccaac caccaagagc gcctccgcca
     1561 cgccgtagaa acaggggcga aagcgtacct agaagcaggt gcaagagagg ttctcgtacc
     1621 gaccgcgcca ccactgcaga tccgttcgtc gagtgatgcc aagaaggcca gtcagctaac
     1681 cttcaagccg gccacggcgc cgctgatcag cgcgcaccag cagggcaccg tgcgcttcgc
     1741 cgcatcggag cgcgacggcg gcgcaaatct cgacggcgag gtctatggta cgcgcggcgt
     1801 ctacgtcttc gactcctcgg gctacccgac cagcgcctcg agccacacca tgaccccgat
     1861 catcgcctcg tcgcgcatgc tcacgacgaa gctcttgact cgaatctagg catgctgcgc
     1921 ccatgaagtc gagcctgaaa cgcggccatg cgtcggtgaa tccactcaat gggatttcgg
     1981 tggcgcggct ttgccatacg cccgtttgag tcgtacaacc tgaacgatga tggttcccga
     2041 ggagatcgac ctgaacgagc tcaggcgcca actggccgca cgcttcggtg atgcgccccc
     2101 tgcgggttac gtctgcggaa agggcgacct ccgcgccgcc gtcgtagaca ttctcggatg
     2161 ctccgagctc gaagccgaac agctcgtcga tacgctcgaa agccgccacc tgattcgcta
     2221 cgagggcgac cggagcgacg gcatcgaccg gctggagacc cactggctgc tgggaaacga
     2281 ttgagctggc gtccaaatcg gctatcgtcc ctcgctatga aacgctttgc acttgccttc
     2341 actttgatca gcctcgtagc gggctgcacg agctccgacg agtgcgccga tctcggcagt
     2401 acctgccccg ctctagaaca gcgcgtcgaa caactgctct ccgagatgac actcgaagag
     2461 aaggtcgcgc agatgtctgg aacgacgttc atcagcggac tctatgggac cgagccttgg
     2521 aatgttcccg gggtcgaacg tctcggcatt ccgcctttca aaatgtccga cgggccgaga
     2581 ggtgttggcg tccaggatgg atccaccgcg tttcccgtgg ccatcgcacg gggagccagc
     2641 tgggacccag agctcgagcg ccgagtgggc gaggcgcagg gtcgcgagct ccgcgccatc
     2701 ggggggaacg ttcttctcgc gccgacgatc aacaacctgc ggcatccgag ctggggacgg
     2761 tctcaggaaa cgtacggcga agacgtgcac cttctctcgc gcttcgcggt ggcctcggtc
     2821 cagggtctgc agaagtacgt gctcgcgaac ccaaagcact tcgccgccaa cagcattgaa
     2881 cgcactcgct ttcaagtgga cgtgagcctc gacgagcgga cccttcgcga gatctatctc
     2941 cctcatttcc gggcggccgt gcacgaaggc ggtgcagcgt cgatcatgag cgcttacaac
     3001 tccgtcaacg ggcaattctg cggtgagaat gaagtccttt tgagaggcat cttgaagggg
     3061 gactggggct tcgacggttt cgtgctttcc gacttcacac tcgggacgca tcccgattca
     3121 gcggtcaacg gactcgacct cgagatgccg acggcgaaca tctttcggag ccttctgcag
     3181 caggtgcgct ccggcgaggt gcccgaagag gtcgtggacg acgcggtccg gcgcatggtc
     3241 cgtaagaaac tccaatacaa gctcgataaa ccgagcgcgg tggatgaaag cgtgatcgca
     3301 agcgacgagc acctcgcgct cgcaagagaa gcggcagtcc gtggttcagt cttgctcaag
     3361 aacgactcga atgccttgcc actcgaccga tcggtcctca cgcgcatcgc ggtcgtgggt
     3421 gagctcgcag atgcgatcaa catcggagac aacggcagca gtagcgttcg tcccgccttc
     3481 gtggtgacgc ccttggacgg gatccgggcg gcagttggcg acgacgtctc ggtggatttc
     3541 atcgggaccg acacactgaa cggcaacgac cttgcgacgc tggaagccgc ggatgcggtc
     3601 atcgtggtaa cgggcctcac cttggatgac gagggcgaag gcttgatcgg cgcgggcgac
     3661 cgtatcgacc tcggcctctc gccggaacgc gtggcactga tccaagacgt ggcggcggcg
     3721 aacgcacgca ccatcgtcgt attggaaggc ggcggcgcta tcacgatggg cgactggatg
     3781 ccggacatcg aagcactgtt gatggtttgg tatcccggac agatgggcgg tcacgccatc
     3841 gcggacctct tgttcggcat cgagaatccc tcgggcaagc tgccgatcac cttccctgtc
     3901 agcctcgatc aactccccga gttcgataac gagtcgcttg ccgttaccta cggctacttc
     3961 cacggctacc ggtacgtgga tctcaacggt acggaccccg aatttccgtt tggcttcggg
     4021 ctcagctaca cgacgttttc ggtcgctaat cttgctgcga gccaagcgca ggcgagtgcc
     4081 ggcgacgtgg tgcgcttcag cgtcgacgtg accaacgacg gccccgtagc cggctccgag
     4141 gtggtgcagc tgtacgtgac ctaccccaac tcctccgtgg aacgctcggt gcgcgagttg
     4201 aagggtttcg cgaaagttga gctggagccc ggggccactg agacagtcac gatcgagctg
     4261 cgggtcaacg acctggcgta ctacgacgtt gccgcgtctg cctgggccct cgaagcgttg
     4321 gaacacgagg tgctgatagg gacgtcgtcg cgcgacctac cgctcgcaac gaatctctcg
     4381 gtgaacgcgg cggcgccggt cgaggtgtac tgatctcgat ttccgggcta ccttgagcgc
     4441 cgttcgtcaa aggagaagtg gatgcaaagg ttcgttcaaa tagccgctgc ctcggtcctc
     4501 gtcgtcggag ctacggtggg ggtttcctct gcagccgaga tcggtttccg cagcgtcgac
     4561 tttccaacca cgggagggat ccaaggccga gcggacgtct atgagtcgca ggacagctcg
     4621 gcgacgctga tcctcctctt tcatcaggcc ggctggagcc gaggtgagta tcgagagatc
     4681 gcgccacagc tcgtcgagcg tggctatcgc gtgatggcgg tcgaccagcg ctcaggcggc
     4741 ggcgtcaacg gagtgccaaa cgaaacgcat cgtcgagccg ctaagaaggg cctcggaacg
     4801 agctatctcg acgcctacgc agacctagag gcagcgctcg cctatgcaca cgaagcgctc
     4861 aaagccgagc gagtgatcgt ttggggaagc tcgtattccg cctccctggt ttttcgattg
     4921 gctgccgagc atgctcgaga tgtggcagcc gtgatggcgt tttcaccagg cgaatacttc
     4981 caaaagggag tgggtgctga gtacgtccgc ggcttcgcca agcgggttga ccagcccttg
     5041 ttcgtgacct cggcgaaaac ggaacgcgag caggcgaaac ccatattcga cgcatcgcct
     5101 gcgacgaaga agatcctatt cacccctgca tccatgggtc agcacgggtc tcgtgcgctt
     5161 tggaagaagt ggaaggatca ggacgtctac tgggcggcgg ttggcgcctt cctgaaagag
     5221 tacgcgccgc ccacgccacc aaagtgaaac cgaggtcgct ggaaacgctg cgaagctgag
     5281 ctacatgggt cctgtgactc agctcccggt gcagcgtgtg atggacaccg aacgggcgcg
     5341 tcttgccggt cgacagctcg gcgtcgacgt cgatattcat agccagctcc ggcccttcga
     5401 agcgcatcgc tggcgcgaag gcgactcgcc tcaccccact ctgcatctcg acgacgtgtc
     5461 cgcaatcccg ttcctcgccg acattgcagg gatcgaggag taccaacacc ggggccggct
     5521 ccgcgccggc gacggagaca tctatgtgac catcacgccc gcaggtgaag gctacgagtc
     5581 gtactgccgt cacacattgc ggatgggaca tccggagcac ctcgtggccg atccgctcgg
     5641 cccggcgatc gccgtcgccc gctcctgcaa ggtgggcgcg gcacacgctc gcctggtcga
     5701 agcgactcga gagtccggcg gattgacgat cc
//
LOCUS       DASRZF010000206         4445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3236628, whole genome shotgun sequence.
ACCESSION   DASRZF010000206 DASRZF010000000
VERSION     DASRZF010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3236628"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..1139
                     /locus_tag="VFG22_09220"
     CDS             114..1139
                     /locus_tag="VFG22_09220"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6416463.1"
                     /translation="MSNHEILVTGGAGYIGAVLVPELINDGYSVTVLDAFLYGTDPSR
                     DPIVHERCRKIRADIRDFDFVDSVLKEGRFDTIIHLAAISNDPSSALDPDVTRSVNLE
                     AVDHLMHSAKRHRIKRFLYASSASVYGIKDTENVTEDLPLDPITIYAKCKSEGETILN
                     DLVDESFVGVSVRSATVCGYSPRLRLDLTINLLTDQGLTDQRIRVFGGDQMRPNVHVR
                     DLSAFYRTLITAPAEKISGEAFNVCRENASVMGLAEMIRDELDPALPIDTVPSDDPRS
                     YHLCADKALNLLGFEPSRDLVTAVKELRQAYDSGKVPDSQSEVYRNVVWMKARPDVWK
                     NVTKRAS"
     gene            1136..2242
                     /locus_tag="VFG22_09225"
     CDS             1136..2242
                     /locus_tag="VFG22_09225"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HET6416464.1"
                     /translation="MRAVPYVDLVKQNASLRSQIAEAVDRVIRHGHFTNGPEVEQFES
                     ALATELGIENVIGVGSGTAALTLVMRAFDIGPGDEVITVAHSYVATASAILLVGATPV
                     FVDVDPDTALMDPKLLLNKLTPRTKAIVPVHLGGVACDLEPIERFCAARDLHLIEDCA
                     QAIGATYQGRSVGSVGTGCFSLHPLKSLAACGDGGFITTNDSELAALLRKLRTLGHRD
                     RDHVDYISENARLDTLQAAILNVKLPHLNDWIGARRNHGAVYQRALARYFELTSITES
                     CEPAFSAFVLRHDARDELLSHLADHGFDAKVHYPIPIHKQRAFASLAPVSLPHTESLA
                     RRILSLPVSPELDPMDRDALVDVLIAWSKARESS"
     gene            2229..3782
                     /locus_tag="VFG22_09230"
     CDS             2229..3782
                     /locus_tag="VFG22_09230"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET6416465.1"
                     /translation="MRAPEVERKIFEVDDLGVERAKLRANGKTVVQCHGCFDIVHPGH
                     IRYLRFARSLGDVLIVTVSSDDVVMKGYDRPYIPEDLRLDNLAELACVDYVALSKDEW
                     AGPVLEALQPDIYVKGREYESKSDPRFANEKTIVEAYGGRVVLGSGDVIFSSTEIGRR
                     KGNTLDMEQQKIQRFCRINEITPARLKNAISRFSDLRILVLGDAIIDHYSHCEGARVA
                     SESPIVSVKPVTDEWFVGGAGLIVRQASALGAKATFVTCSAEDEGLDRIRRTLRRFGA
                     EVHNLEVRGRGSYTKTRFIVGGKKVFKVDRGNPSPIPIETTTELIGKLGELLESHDGL
                     IVTDFGYGLFGSTLCDAISELASSAGKPYFADVSTNGQANILKFRRPHLATPTEDELR
                     FALADAESGISNLASRYFAASDARGLIVTMGRRGCLSFDPPGKQSQRLRTHYLPALEV
                     NEVDAVGAGDVFLAGSSLAQLAGEPVPVGMYIGSSLASISAGILGNEGAPLPDVYDFF
                     EQRVELAGE"
     gene            3779..>4445
                     /locus_tag="VFG22_09235"
     CDS             3779..>4445
                     /locus_tag="VFG22_09235"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HET6416466.1"
                     /translation="MSFRRGGPRRWLILGVGLSISGLALYFLLSRVDGTQLVSALRSA
                     NPWILSVCLLTKGSVLALNAARTRILLLPLRRYSFVDCFIPWLSGYVTDNLLPFRLGE
                     LVRIDLLARAGEVSRSSTIAVVGLDRLIDLMSMLLLFAVAAPLLTFDLARADRLAIVT
                     FIIVAGLALALWLATHPSAISRGTATLVRPLAPAIQQWLVEKAQRFSEGLGALRSRSM
                     IASV"
BASE COUNT          884 a         1347 c         1297 g          917 t
ORIGIN      
        1 tgcagcgcag ctgaatccac caggacgcca tccatgtcga agaggacccc ggttgtcgtc
       61 atttttcatt caccctgcag ttaggatatc ttacttgaac acaaggggtg gaaatgtcga
      121 atcacgagat tctcgtcacg gggggcgcgg gctatatcgg cgccgtgctc gtgccggagc
      181 tgatcaacga cggttacagc gtcaccgtcc tggacgcgtt tctctacggc accgatccga
      241 gtagggaccc gatcgtacac gagcggtgcc ggaaaatcag ggcggacata cgagatttcg
      301 acttcgtgga ttccgtgctc aaggaaggcc gcttcgacac gatcattcat ctggcggcca
      361 tctcgaacga tcccagctct gcgctggacc ccgatgtgac ccgcagcgtg aatctcgaag
      421 ccgtggatca tctgatgcat tcggcgaaac gacaccgcat caagcggttt ctctacgctt
      481 catcggcaag tgtctatggc atcaaggaca cggagaacgt aaccgaggac ctgccactcg
      541 atccgatcac catctacgcg aaatgcaaat cagagggtga gaccatcctg aacgacctcg
      601 tggacgaaag cttcgttggc gtttcggtcc gttccgcaac cgtgtgcggt tactcgccac
      661 gcctgcgtct cgatctcacc atcaacttat tgactgacca gggcctgacc gatcagcgaa
      721 ttcgcgtgtt cggcggagac cagatgcggc ccaacgtgca cgtccgggat ctcagcgcat
      781 tctaccgaac attgatcacg gctcccgcag aaaagatctc aggagaagcc ttcaacgtct
      841 gccgggagaa cgcttcagtc atgggcctcg ctgagatgat ccgcgacgag ctcgatccag
      901 cactgccaat agataccgtc ccatcagacg acccacgttc gtaccacctc tgcgcagaca
      961 aggcactcaa cttgcttggc ttcgagccga gccgcgatct agtcacggcg gtcaaggagc
     1021 ttcgccaggc ctacgattca ggcaaggttc ccgacagcca gtccgaggtc taccgcaacg
     1081 tggtatggat gaaggcccga cccgacgttt ggaagaacgt cacgaagcgt gcttcatgag
     1141 ggctgttcct tacgtcgatc tggttaaaca gaacgcatcg cttcgctcgc agatcgcaga
     1201 ggccgtcgat cgagtaatcc gtcacgggca tttcacaaat ggtcccgagg tggagcagtt
     1261 cgagagcgcg ttggcgaccg agctcgggat tgaaaacgtc atcggagtcg gatctggaac
     1321 ggcagcactg acgctggtga tgcgtgcttt cgacatcggt ccaggagacg aagtcatcac
     1381 tgtcgcgcac tcgtatgtgg ctacggcttc cgcgatcctc ttggtcggcg ctactccggt
     1441 cttcgtcgat gtcgatccgg acaccgcgct catggatccg aaactgcttc tcaacaagct
     1501 cacccctcgc accaaagcca tcgtccctgt ccatctgggc ggggtagctt gcgatttgga
     1561 gcccatcgaa cgcttctgcg cagcgcgtga tttgcacctg atcgaggact gtgcccaagc
     1621 gatcggagcg acataccagg gccgcagcgt cggcagcgtt ggaacgggct gcttctcgct
     1681 tcatcctctg aagtcgctgg cagcctgtgg cgacggcgga ttcatcacga cgaacgactc
     1741 cgagctcgcc gcgctgttgc ggaagctgcg cacactggga catcgcgacc gtgatcatgt
     1801 cgactacatc agtgagaacg ctcgactcga cacgcttcaa gcagcgattc tcaacgtgaa
     1861 attacctcat ttgaacgact ggatcggggc gcggcggaac catggagcag tgtatcagcg
     1921 agcgctggcc cgatatttcg agctgacttc cattacggag tcgtgcgagc ccgccttcag
     1981 cgccttcgtg cttcgacacg acgcacgcga cgagctgttg tcgcatttgg cggatcacgg
     2041 attcgacgcg aaagtgcact acccgattcc gattcacaag cagcgtgcat tcgcctccct
     2101 tgcgccggtg agcctgccac acactgaatc tttggctcga cggatcctga gccttccagt
     2161 atcgcctgag ctcgatccga tggatcgaga tgcgctagtc gacgtactaa ttgcatggag
     2221 caaagcccgt gagagctcct gaggtcgagc gcaagatctt cgaagtggat gacctgggcg
     2281 tcgaacgggc caaactgcgc gcgaatggaa agaccgtggt tcagtgccac gggtgcttcg
     2341 acatcgttca tcccggtcac attcgatacc tgcgtttcgc gcgcagcctg ggtgacgtcc
     2401 taatcgtaac cgtgagtagc gacgatgtgg tgatgaaggg ctacgatcgg ccctacatcc
     2461 ccgaagactt gcggctagac aatttggcag agctagcctg cgtcgattac gtggccctga
     2521 gcaaagatga atgggcgggc cctgtcctgg aggcgctgca gcccgacatc tacgtaaagg
     2581 gccgtgaata cgagtccaag agcgaccctc gcttcgcaaa cgagaaaacg atcgtcgaag
     2641 cctacggcgg acgggtggtc ctgggatcag gcgatgtaat cttctcgtcg accgagatcg
     2701 gccggcgcaa gggcaacacg ctcgacatgg agcaacagaa gattcagcgc ttctgtcgaa
     2761 tcaacgagat cacgcccgcg cgcctgaaga acgccatatc gcgcttctcc gatctacgga
     2821 tcctcgtgct gggagacgcg atcatcgatc actactcgca ctgcgaaggg gcgcgagtcg
     2881 cctccgagag cccgattgtg tccgtcaagc ccgtcactga cgagtggttc gtgggaggcg
     2941 ctggactgat cgttcgacag gcatcggcgc tcggtgcgaa ggcgacattc gtaacctgct
     3001 ccgcggaaga cgagggcctg gaccgcattc gacgcacgtt gcgtcgcttt ggagcggagg
     3061 tccacaactt ggaggtccgt ggacggggtt cgtacacgaa aactcgcttc atcgtcgggg
     3121 gaaagaaagt cttcaaggtc gaccgtggca acccctcacc cattccgatc gaaacgacga
     3181 cggagctcat cggtaagctt ggagagttgc tcgaatcaca cgacggactg atcgtcacgg
     3241 acttcggtta cggcctgttt ggcagcacgc tgtgcgacgc tatttcggag ctcgcttcat
     3301 cggccggcaa gccttacttc gcggatgtca gcaccaacgg ccaggccaac atcttgaagt
     3361 tccggcggcc tcatcttgcc acaccgacgg aagacgagct tcgtttcgcc ctggcagatg
     3421 ccgagagcgg catttccaac ctcgcaagcc gctacttcgc cgcaagcgac gcacgcggcc
     3481 tcatcgtgac gatgggacgc cgcggctgtc tatcgttcga cccacccggc aagcaaagcc
     3541 agcggctacg aacccactat ctgcccgcat tggaggtcaa cgaggtagac gcagtcggcg
     3601 cgggtgatgt ctttctggca gggtcgagcc tggcacaact cgcgggcgag ccagtgcccg
     3661 tggggatgta catcggaagc tcgcttgcat caatatcggc cggcatcctc ggaaacgagg
     3721 gggcgccgct tcccgatgtc tacgacttct tcgaacagcg cgtggagctc gctggcgaat
     3781 gagctttcgg cgaggcggcc ccaggcgatg gctgattctc ggggtgggac tctcaatctc
     3841 ggggcttgct ctctatttct tgctgagtcg cgtcgacggg acccagctcg tctccgcgct
     3901 gaggtcggca aacccttgga ttctctccgt ctgtctcttg acgaaaggtt ctgtgctcgc
     3961 tctcaacgcc gctcgaactc gaattctgtt gctaccgctt cgtcggtata gcttcgttga
     4021 ttgcttcata ccttggctct cgggctacgt caccgacaac ctcttgccct tccgccttgg
     4081 cgagctggta cgcatcgacc tcttggctcg agccggcgag gtgtccagaa gctcgacgat
     4141 tgcggtggtc ggactcgatc gcctgatcga tctgatgtcg atgctgctct tgttcgccgt
     4201 cgctgccccg ctcctgacct tcgatctcgc ccgggccgac cgcttggcta tcgtcacgtt
     4261 catcatcgtc gctggcttgg cgctggccct ttggttggct acacatcctt cggcgatctc
     4321 gcgtggtacg gcgacactcg tacgtcccct agcgcccgcg attcagcaat ggctagtcga
     4381 gaaggcacaa cggttctccg agggcctcgg agccctgcgt tctcggagca tgattgcctc
     4441 cgtga
//
LOCUS       DASRZF010000207         7052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4272354, whole genome shotgun sequence.
ACCESSION   DASRZF010000207 DASRZF010000000
VERSION     DASRZF010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 7052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7052
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4272354"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            294..818
                     /locus_tag="VFG22_09240"
     CDS             294..818
                     /locus_tag="VFG22_09240"
                     /inference="COORDINATES: protein motif:HMM:NF042800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding protein"
                     /protein_id="HET6416467.1"
                     /translation="MAATFVVETGTGSSTANSYSTEAAADQYHENYGDPTDWSGATTT
                     VKENALRVATQYMDARFGTRWLGTKANETQRLDWPRCGAVVEEYTIDSDVIPEPLQDT
                     TAVLALKHVQGTALIPDVTNAGTLSRVRVKVGPIEEEKEYAGSFGETDQTTFELAERL
                     IASLVWPVGWAVRG"
     gene            815..1192
                     /locus_tag="VFG22_09245"
     CDS             815..1192
                     /locus_tag="VFG22_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416468.1"
                     /translation="MTGALDATARAVAGRLIDKYGKALTFTSAATTYDPDSDVVVGAG
                     TEYEWNGVLDKFGRAYPLRVTEKHGMTIIYVPVVNTAGTVATFTPEAGWTVTVDAEEF
                     RVVAVDEVKSGELGAMWILETNK"
     gene            1195..1626
                     /locus_tag="VFG22_09250"
     CDS             1195..1626
                     /locus_tag="VFG22_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416469.1"
                     /translation="MPVVTEAQIEAARFKTFESAASFNRAVADFADSIGPTMLVPFHK
                     KLHLQGLRGIVQKTPVDTGRARGNWQSSRDIEPVGELGIRSGGDAVSEGVTVTRTLAP
                     YTVSYITNNLPYIEFLEAGGSDQTPAGMVSVTITELETQFP"
     gene            1623..2033
                     /locus_tag="VFG22_09255"
     CDS             1623..2033
                     /locus_tag="VFG22_09255"
                     /inference="COORDINATES: protein motif:HMM:NF024943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator-like protein"
                     /protein_id="HET6416470.1"
                     /translation="MSYAAIHDAIRTRFKNLVADAESVPVAYPNAPFETPDDQSMWVR
                     LSILDGQSSQIELEANDGSTYRRVGNLIAQIFAPAQQSTGDAMDLADVIAAAFRRVYA
                     IPVRYRVPSVQTIGRSNGWWQVNVVCPWESELIS"
     gene            2089..3258
                     /locus_tag="VFG22_09260"
     CDS             2089..3258
                     /locus_tag="VFG22_09260"
                     /inference="COORDINATES: protein motif:HMM:NF039819.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HET6416471.1"
                     /translation="MADTDKVSLAYEEETVEGTINATPATTLARLTGESLKQNTTTQI
                     PDELDPAAQIATAPVRTDVSAGGDINYLLSHATYNDFIEATYRSTWSAAVVVEAASAL
                     IAATNSTTLTLSAGAWDNTPVVGSWIEVSGFDGQTETVMYTRVSAATTTTITSAFAIF
                     TAEAEGDSVTITQLARVTNGTTERAFTLEKKYLDADGAGTDEYAQYVGSVFGGMTLNA
                     AIQASMAGSFSVLSRTEISASLPLDASPVQAPTGEPYSTVSDVPFVLESTAAITTATE
                     LNSTVTNNLRSEPAIGSLGAHRVGRGRFEASGTLKRYMLSEALIDKYLNYTTSGLALV
                     CVNSDGATIFDYPSVKYTDGQRLAGGPNQSVLTELTWQAFKDATLGFSSQVAKTA"
     gene            3337..3687
                     /locus_tag="VFG22_09265"
     CDS             3337..3687
                     /locus_tag="VFG22_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416472.1"
                     /translation="MRQAPDDRNGEDVWYMCVSPRADRKVMRIVRDLAQDLTERKGKE
                     LSKEETEAIGRAAHVVDWENLRDDDGAEIECTPERVFRLLVEECPEYGVDVQLMMQLA
                     AFRTGRSEAAEEGK"
     gene            3825..4037
                     /locus_tag="VFG22_09270"
     CDS             3825..4037
                     /locus_tag="VFG22_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416473.1"
                     /translation="MILHRSRDGTAPIRATEIEAEARIRGIEDVEDFARLIRVMDSEW
                     VAYVEEQRNADSEPRYRRSSSSARRR"
     gene            3982..7005
                     /locus_tag="VFG22_09275"
     CDS             3982..7005
                     /locus_tag="VFG22_09275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HET6416474.1"
                     /translation="MPTLSLAIDARRARQGAGEFEQATGRVQRATTSALRSVTALAGG
                     ISAAFAARKIVTDAARFEQGLVGVGKTADLSGAALKALGQDVLELSTRIPVATEELLG
                     IAQAAGQLGVTGSANILKFTDTVAKLGTASDLSGEAAATTLARLLNVTNESVSGVDRL
                     ASVIVRLGNEFAATESEIAAVGQRVAQSTAAFDVTSAQAVALGAALRSAGVEAELGGT
                     EVGRAFRLIEAAVRDGGEELQKFGELTGKSGREFADAFKSSNIRGFQLFIDGLARVAA
                     EGGSVSGTLQDIGLDGARSSAVFATLAATSSELGRALSTANDELSNTTALETEAARGA
                     DTLSGDWQRLLNTIKAVSVEAGDAGLLGALRDIVQTTSDVVRVLAGLETGFQKSRERA
                     ELLVSAVKAFSAALLTIPARAAAAAIAAIASSAAAATGSVTALAFATQSLVAFITGPV
                     GIAVAVGAAVFAFDQLRAEAVDLKLELSEIASVTSEAASAQQAYNAALKDGAIDLQVL
                     ALRRQQDALLAVASELREVRAVAGSRAEAEFEVVANVTGLRRDEIEAVVARELGAGVD
                     ADLRFSDRVSDDLAKQILRVLEEVPEIDLGDRLTLGASAARIKEISAELTDLVKATGS
                     GERAVALLLERYGVDRLPIEAALAAVGDAFDDLEGKIATAGTGAAAGALGGLAESAGK
                     AMGELDRLGRTQRIVAASTSELGEGSQDLAGAFGLLGSTIARQTAEAAVLRATWDTIP
                     IVFGVAKAALKEYRDEADQAARLDAFAELKRNAASAREELARLEESLRLEIVAQSPGG
                     GVLEADLIRVRSLAQAAGEDADKAAERFQALFDELDALEQSRLFAEGIVDPIINGLDE
                     AITRGKNLGEVLEDIARNIFRNLFNQLALQPLQESLVNIVQGAAGGLFSAKGNVFTPG
                     FGGPQVVPFASGGILNGPVNFPLAGGRRGIAGEAGPEAIMPLERDGSGRLGVRSQGGG
                     GQSIVFNITTPDADSFRRSERQIYRNAQRQLRGAG"
BASE COUNT         1368 a         2052 c         2319 g         1313 t
ORIGIN      
        1 gtgcaagcgc aagtaatgcc cacttccatc ctcccccgca aatcgcttga tgagaacggc
       61 cgcgctcgcg tgtggctgct cgacgagaag cgatggcata cgtgttggcc tgttgatgcg
      121 cgcgagatga tcgatctcgg acaagcagaa ttggacgagc ctgtcaccaa ggccgctccg
      181 ccgcctccgc gcacccctgc gccggctccc gcaacgccgc cggggccgaa gtagtcccct
      241 ccccggggcg cgtggcgtga ttccttctag cgtcacgcgc ccacctatca cctatggccg
      301 ccaccttcgt agtcgagact ggtactggat catcgaccgc taattcctat tcaacggaag
      361 cggcggcgga tcagtatcac gagaactacg gtgacccgac tgactggagc ggtgcgacta
      421 cgacggtcaa agagaacgca ttgcgggttg cgacccagta catggacgct cggtttggca
      481 cgcgatggct gggcacgaag gcgaacgaaa cgcagaggct cgattggcct cgatgcggcg
      541 ccgtggtcga ggagtacacg atcgacagcg acgtaatccc tgagccgttg caagatacta
      601 cagcagtcct tgcactaaag cacgttcagg ggacggctct gatccccgac gtgacgaatg
      661 cgggaacgct gtcgagggtt cgcgtcaagg tcggtccgat cgaggaagag aaagaatacg
      721 ccggcagctt cggcgagacg gaccagacga catttgaact ggcggagcgc ctgattgcat
      781 cgctcgtgtg gcccgtgggc tgggcggtgc gcggatgacc ggcgctctgg acgccaccgc
      841 acgcgctgtt gcgggccggc ttatcgacaa gtacggcaag gcgctgacgt ttacgagtgc
      901 ggcgacgacg tacgacccgg actctgatgt ggttgtcggc gcggggactg agtacgagtg
      961 gaacggcgta ctagacaagt tcggccgcgc ctatcctctg cgcgtgactg agaaacacgg
     1021 gatgacgatc atctacgtgc ccgtggtgaa cacggccggt acggtggcga cgttcactcc
     1081 cgaggctggc tggacggtga cggtcgacgc ggaggagttc cgtgtggtcg cagtagatga
     1141 ggtcaagagt ggcgagcttg gggccatgtg gatcttggag acaaacaagt agcgatgccc
     1201 gtcgtcacgg aagcccaaat cgaagccgcg cgtttcaaga cgtttgagag cgccgcgagt
     1261 ttcaaccgtg ccgtcgcgga ctttgcggac agcattggcc cgactatgct ggtgccgttc
     1321 cacaagaagc tgcaccttca aggactgcgc gggatcgtgc agaaaacacc cgttgatacc
     1381 gggcgcgcgc gagggaattg gcagagcagc cgggacatcg agccggttgg cgaactgggc
     1441 atccgcagcg gcggggatgc ggtcagcgag ggcgtcaccg tgacacgtac gcttgccccc
     1501 tacaccgtct cctacatcac gaacaacctg ccctacatcg agttcctcga agcggggggg
     1561 tccgaccaga cgcccgccgg catggtctct gtaacgatca ccgaactgga gacgcagttc
     1621 ccgtgagtta cgccgccatc cacgacgcga tccggacgcg gttcaaaaat ctagtcgccg
     1681 acgccgagtc ggttcctgtt gcgtacccga acgcaccgtt cgagacgccc gacgatcagt
     1741 ccatgtgggt tcgcctgtcg atcctcgacg ggcagtcctc gcagatcgaa ctagaggcga
     1801 acgatggttc tacataccgg cgcgttggga acctcattgc tcagatcttc gctccagctc
     1861 agcagagcac gggcgatgcg atggaccttg cagacgttat tgcagccgca ttccggcgcg
     1921 tgtacgccat cccggtccga taccgagtcc cttccgttca gaccatcggc cgaagcaatg
     1981 gctggtggca agtcaacgtc gtttgtccct gggaatccga actcatttcc tgaccgaacc
     2041 cttggcggtg cccgatagtg ccctgccgca acagaagaga gcactgatat ggctgatact
     2101 gacaaggtaa gcctcgccta cgaggaagag accgtagagg ggacgatcaa cgcaaccccc
     2161 gcgacaactc ttgcccgtct aaccggcgag agcttgaagc aaaacacaac cacgcagatt
     2221 ccggacgagc tggaccccgc tgcccagatt gcgacggctc ctgttcgtac ggatgtcagt
     2281 gctggcggcg atatcaacta cctgctatcg cacgcgacct acaacgactt catcgaggcg
     2341 acgtatcgga gcacgtggtc ggcggctgtt gtggtcgagg ctgcgagcgc gctcattgcg
     2401 gcaacgaaca gcacgactct tacgctgagc gctggggcct gggacaatac cccggtggtt
     2461 ggatcttgga ttgaagttag cgggttcgac ggtcagaccg aaacggtcat gtatacccgc
     2521 gtttcggcgg ctacgacgac aaccattacg agtgcgttcg cgattttcac ggcggaggcc
     2581 gaaggtgatt cggtcacgat cacccagctc gcccgcgtaa ccaacggaac caccgagcgg
     2641 gcattcacac tcgagaagaa gtacctcgat gcggatggtg ccggaacgga cgagtacgcc
     2701 caatacgtcg gatcggtgtt tggtggcatg acgctgaatg ctgccattca agcctcaatg
     2761 gccggctcgt tctctgtgct gtctcgtacc gagatcagcg catcgttacc gctggacgct
     2821 tcgcctgtgc aggccccgac tggcgagccg tattcgacgg tcagcgacgt tccgtttgta
     2881 ctggaaagca cggccgcgat cactacagca accgaactca actccaccgt cacgaacaac
     2941 ctgcgttctg agccggctat cggttcactc ggcgctcaca gggtcggacg tggcagattc
     3001 gaggcgagcg gcacgctcaa gcggtacatg ctgagcgaag cgcttatcga caagtacctc
     3061 aactacacga cctctgggct cgccttggtg tgtgtcaact cggacggagc aaccatcttt
     3121 gactatccga gcgtcaagta caccgatggg caaaggctcg ctggtggccc taatcagtcc
     3181 gtcctgacgg aactcacttg gcaggcgttc aaggacgcca cgctcggctt ttcttctcag
     3241 gtcgccaaga cggcatgatc cagtatgccg acgattagcg aactcaaggc cgcgtcaagc
     3301 caagcccgga ctggggtatg gtttgatgtg gccgagatgc ggcaagcgcc cgatgatcgg
     3361 aacggcgagg acgtctggta tatgtgcgtc tcgccgcgtg ccgatcgcaa ggttatgcgt
     3421 atcgtgcgcg acttggcgca agaccttacc gaacgcaagg gtaaggaact cagcaaggaa
     3481 gaaaccgagg ccattgggcg cgcggcgcat gttgtcgatt gggagaactt gcgcgacgac
     3541 gacggtgccg agatcgaatg cacgcctgag cgtgtgtttc gcttgctggt cgaggagtgc
     3601 cccgagtacg gcgtcgatgt ccaactgatg atgcagcttg cggcgtttcg tactggccgc
     3661 tcggaggcgg ccgaagaggg aaagtagcag ggtggctgcg gcacaagctc actaccgagc
     3721 cgcttcgaaa ggagctcgag caactcgcgc gatggggtgc tatcgaggca ccggagccac
     3781 ctccgctact tcctatctgg ctcaaggatc ccgtagaggc gtggatgatc cttcatcgct
     3841 ctcgcgacgg gactgctcct atccgagcga ccgagatcga ggccgaggcg cgcatccgcg
     3901 gaatcgagga cgtggaagac ttcgcacggc tcattcgtgt gatggactcc gaatgggtcg
     3961 cctacgtgga ggagcaacgc aatgccgact ctgagcctcg ctatcgacgc tcgtcgagct
     4021 cggcaaggcg ccggtgagtt cgagcaggct accgggcggg tccagcgtgc cacgacttcc
     4081 gcgctacgca gcgtaacggc acttgccggc ggtatctcgg ccgcgtttgc ggcgcggaag
     4141 atagtcactg acgccgctcg cttcgagcag ggattggtgg gtgtcgggaa gacggccgac
     4201 ctgagcggcg ctgcgctcaa ggcgttgggc caggacgtgc tcgagctttc aacgcgcatc
     4261 ccggtcgcga cggaggaact gctcgggatc gcgcaggctg cgggtcaact tggggtcacg
     4321 ggctcggcga acatcctcaa gttcaccgac accgttgcaa aactcggcac cgcgtcggac
     4381 ctttcgggtg aggctgccgc cacgacgctt gcccgacttc taaatgtcac gaatgaaagc
     4441 gtatcgggcg ttgaccgtct ggcttctgtt atcgttcgtc ttggaaacga gttcgcagcg
     4501 accgagagcg agattgcagc ggtcggccaa agggttgctc agagtacggc ggcgttcgac
     4561 gttaccagcg cgcaggccgt tgcgcttggc gcggcgctgc ggtcggcggg cgtcgaggcg
     4621 gaactgggcg gtacggaggt cggacgggcg ttcagactga tcgaggctgc cgtccgggac
     4681 ggaggagaag aactccagaa gttcggggaa ctgaccggca agtccgggcg tgaattcgcg
     4741 gatgcgttca aatcatcgaa catccgtgga ttccagttgt tcatcgacgg acttgctcgg
     4801 gtcgccgcgg agggtggatc ggtctcgggc acgcttcaag acatcgggct cgacggcgcg
     4861 cggtcgtccg cggtgtttgc gactttggcc gcgacgagta gcgaactcgg acgcgctctt
     4921 tcgaccgcga acgacgaact atcgaacacc accgcgctcg agaccgaagc tgcccgcggc
     4981 gcggacacgc tctctggtga ctggcagcgc cttctgaaca cgatcaaggc cgtcagcgtc
     5041 gaggcggggg atgctgggct tctaggcgcg ctacgcgata tcgttcagac gacgtctgac
     5101 gtggtgcggg tgctggccgg gcttgagact ggattccaga agagcaggga gcgggcggaa
     5161 ttgcttgtta gtgcggtcaa ggcattcagt gccgcgctgc tgaccatccc ggcccgagct
     5221 gccgccgcag caattgctgc tattgcttcc agcgcggctg ccgcgaccgg cagcgtgact
     5281 gctcttgctt ttgccactca gtccctggtt gcgtttatca ctggcccagt cgggattgcc
     5341 gttgctgttg gcgcggcagt gttcgcgttc gatcagctta gagcggaggc ggtagacctc
     5401 aagctggagc taagcgagat tgcctccgtc acgagtgagg cagcgagcgc acaacaggca
     5461 tacaacgcag ccctgaaaga cggggcgata gaccttcaag tgctcgccct gcggcgccag
     5521 caggacgcgc tcctggctgt agcgtcggaa ctgcgcgagg tccgcgctgt ggctggcagc
     5581 cgcgcggaag cggagttcga ggtagttgcg aacgttaccg gtctgcgtcg cgatgagatc
     5641 gaggcggttg ttgcgcgcga gctgggcgct ggggtagacg ctgatcttcg gttttcagat
     5701 cgtgtctcag acgaccttgc aaagcagatt cttagggttc tggaagaagt acccgagatt
     5761 gaccttggcg acaggctaac gcttggagca agcgccgcta ggatcaaaga gatttccgca
     5821 gagctaaccg atctggtcaa agcgaccgga tccggtgagc gtgcggttgc tctactcctg
     5881 gagagatacg gggtagaccg gctgcccatc gaggctgccc tagcggctgt tggtgatgcg
     5941 ttcgacgatc ttgaggggaa gattgccacc gctggcaccg gcgcagcggc aggcgctctg
     6001 ggtgggcttg cggaatcggc gggcaaagcg atgggcgagc tcgaccgcct tggtcggact
     6061 cagcgcatcg ttgccgcatc cacctctgag cttggcgagg gatcacaaga ccttgccggg
     6121 gcgttcggct tgctcggatc cacgatcgca cgccaaacgg cagaggccgc agtattgcgt
     6181 gcgacgtggg acaccatccc gattgtgttc ggagtcgcga aggcggcgct gaaagagtat
     6241 cgggatgagg cggatcaagc tgcacgtctt gatgcgttcg cggaactcaa gcgcaacgcg
     6301 gcatcggcgc gtgaggaact cgcacggcta gaggaatcgc tgcggctgga aattgtcgcg
     6361 caaagcccgg ggggtggcgt actcgaggcg gatttgatcc gtgttcgttc gcttgcgcag
     6421 gcggctgggg aagacgccga taaagcggct gagaggttcc aggctctttt cgacgagctt
     6481 gatgcgttgg aacagtcgcg cctcttcgcc gaaggcatcg tcgatccgat catcaacggg
     6541 ctggatgagg cgattacgcg cggcaagaac ttgggagagg ttctggaaga catcgcccgc
     6601 aatatcttcc gcaacctgtt caaccagctc gcgctccagc ctttgcagga gtcgcttgtg
     6661 aatatcgttc agggcgcggc tggaggactg ttcagcgcga agggaaacgt cttcactccc
     6721 ggctttggtg gcccacaagt cgtgccgttc gcgtcgggcg gaatcttgaa cggccccgtc
     6781 aacttcccgc tcgctggcgg tcggcgcggt atcgcgggcg aagctggtcc agaagcgatc
     6841 atgccgctcg agcgcgacgg ctctggtagg ctaggcgtgc gctcgcaggg tggtggaggc
     6901 cagtccatcg tcttcaacat caccacgccc gacgcggaca gcttccgccg gtcagagcgc
     6961 cagatatacc gcaacgcgca gcggcagctt cgaggggctg gctgatgggc ttccacgaag
     7021 tacagttccc tgtgacgatc agctacgggt tc
//
LOCUS       DASRZF010000208         3480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4611937, whole genome shotgun sequence.
ACCESSION   DASRZF010000208 DASRZF010000000
VERSION     DASRZF010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4611937"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VFG22_09280"
     CDS             complement(<1..619)
                     /locus_tag="VFG22_09280"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HET6416475.1"
                     /translation="MIKNRSRWLPISLALASAQLGLLCESKVGGLPVSSSPIAVSQDG
                     ASLWVVNPDSDTVGKIDTASAALIDEIPVGDNPRTLALTEHPGLHWLFVANQDSDSLS
                     RINTASGKTKDLELPRGSAPYGVALTPDSKLLLVTLERTHQLAFVDPRTLKVEGMIPV
                     SRTPRGIAVTASGDRAYLSHFITFEPSNSSFITEIDIASRTVTRTI"
     gene            715..1218
                     /locus_tag="VFG22_09285"
     CDS             715..1218
                     /locus_tag="VFG22_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416476.1"
                     /translation="MERSGFLERSGSWLDVRVRVLFRAPTGSPAGLIGADSRNAGRIF
                     PYIGGEEVNGNPTQAHRRYVINFGQISLEAAEQRPDLMRIVRERVKLDVRDRSAATKR
                     PCRRSSAVISGRAVFELNDGASMELGPGDTIVRNETRHAWCNLHSERCRMAVFGGRQA
                     KMTRNRT"
     gene            complement(1318..1980)
                     /locus_tag="VFG22_09290"
     CDS             complement(1318..1980)
                     /locus_tag="VFG22_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416477.1"
                     /translation="MRFEFTLCIVLIISSPILSEQARAANSVEIVWISSTGSGTPGSS
                     TIDAVAGDRLVAQVRITPDAGGVSGYATTLSFDADLADELDLVSATEMLPPPMALQLS
                     PGVVRAMIESNGAQAGSVGEFEALNLSGGPTTGTMVAGELMFDVTANVATDGDDVTPG
                     FSVIDGMGSNSYLGVTPVFVGAAVNLGATVVPSLSTSAMIVLALLLASFALLGTSIHQ
                     RC"
     gene            complement(2045..2434)
                     /locus_tag="VFG22_09295"
     CDS             complement(2045..2434)
                     /locus_tag="VFG22_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416478.1"
                     /translation="MTNGLGWIVMVAVLSIASEGTAACVGGARVPPPTTDWCVLVPGT
                     QTAADDTDGDGYADQCDADYNQDCYVTVLDDLFGPQGFALRFGSTVPPASAIYDHNGD
                     NIVGIADFSIWTGVFNTTGPLPQMDHQ"
BASE COUNT          673 a         1027 c         1135 g          645 t
ORIGIN      
        1 cgatcgtgcg ggtcaccgtg cgcgaggcga tgtcgatctc ggtgatgaac gaactgttcg
       61 agggttcgaa agtgatgaag tggctgagat aggcgcggtc gccggacgcg gtcacggcga
      121 ttccgcgcgg cgttcgagac actggaatca tgccttcgac cttcagggtt cgagggtcga
      181 cgaaagcgag ctgatgcgtt cgttccaacg tcacgagcaa caatttgctg tccggcgtga
      241 gcgcgacccc gtacggcgcc gagccacgag ggagttcgag gtctttcgtt ttgccgcttg
      301 cggtgttgat gcgggagagg ctgtctgaat cctgattcgc gacgaacagc caatgcagcc
      361 cgggatgctc ggtcagcgcg agcgtacgcg gattgtcacc gaccgggatt tcgtcgatga
      421 gcgctgcact cgctgtgtcg atcttcccga cggtgtctga atcggggttg accacccaga
      481 ggctcgcgcc gtcttggcta acggcgatcg ggctactgga taccggaagg ccgccgacct
      541 tcgattcgca gagcagcccg agttgtgccg aggccagagc cagcgagatc gggagccatc
      601 gagaacggtt cttgatcatc gtgcgcgcct cggttccaca cccccagggt cagtttccac
      661 gccccgctgc agcattgtca agccggctgg aaggggtggg gtggcggctc ccgcttggag
      721 cggtccgggt ttttggagcg gtccgggtct tggctcgacg tccgagtacg ggtcctattt
      781 cgagccccta cggggagtcc ggcgggactg atcggggccg actcgaggaa cgcagggcgc
      841 atcttccctt acatcggcgg cgaggaggtc aacggcaatc ccacacaagc tcatcgaaga
      901 tatgtcatca actttggaca gatcagtctc gaggcggccg aacagcggcc tgatctgatg
      961 cggatcgtgc gcgagagggt caagctcgat gttcgcgatc gatccgcggc aacgaagcga
     1021 ccttgtcgcc gctcctctgc cgtgatctcg ggccgtgcgg tcttcgagct gaacgatggc
     1081 gctagcatgg agctcggccc cggcgacacg atcgtccgga acgaaacacg tcacgcctgg
     1141 tgcaaccttc actcggagcg ttgccgcatg gccgtgtttg gtgggcgcca agcgaaaatg
     1201 acacggaacc gaacatgaca aggcgccgtt acggccacgt cagcgtgccc gagctggtgg
     1261 ctcaccccgt cgagccgcat gacggtggcg gcgcgtggca agaagagcga acactgatca
     1321 gcaacgctga tggatggagg ttccgagtag ggcgaaggag gccaggagca gtgccagaac
     1381 gatcatcgcc gatgtggaga gcgacggaac gacggtggct ccgaggttga ctgccgcgcc
     1441 gacgaagacg ggcgtcacgc ccaaatagga gttgctcccc atcccgtcga tgacggaaaa
     1501 tccaggcgtc acgtcgtctc cgtcagtcgc cacgttcgcc gtcacgtcga acatcaattc
     1561 gcccgccacc atcgtccccg tcgtaggtcc gccagatagg ttgagggcct cgaactcgcc
     1621 taccgagccg gcctgtgcgc cattgctctc gatcatcgcc cgcacgacgc ccggcgagag
     1681 ctggagggcc attgggggcg gaagcatttc cgtcgcgctc acgagatcca attcgtcagc
     1741 cagatcggcg tcgaacgaaa gggtcgtcgc gtagccggag acccccccgg cgtccggcgt
     1801 gatgcgaact tgcgcgacga gtcgatcgcc tgcgacggca tcgatcgtcg agctgccggg
     1861 cgtgcctgac cccgtgctcg agatccagac gatctcgacg ctgttggctg cgcgggcttg
     1921 ctcggagagt atcgggctcg agatgatgag cacgatgcat agcgtgaact cgaatctcat
     1981 tgccgtagcc tccgggtcga cgtcgcgctg ttggcggccc tgcctcgctg ggagatgctg
     2041 cccgtcattg gtggtccatc tgcggcagag gcccagtagt gttgaagaca ccggtccaga
     2101 tggagaagtc agctatgccg acgatattgt ctccattgtg gtcataaatg gctgaggccg
     2161 ggggcacggt tgagccgaaa cggagagcaa agccctgcgg gccgaaaagg tcgtctaata
     2221 cggttacgta gcagtcctga ttgtagtcgg cgtcgcactg atcggcgtaa ccatcgccgt
     2281 ccgtgtcgtc agcagcagtc tgagttccag gaaccaggac gcaccagtct gtagtcgggg
     2341 gaggaactcg agcaccgccc acacacgcag cggtgccctc ggacgcgatc gaaagcacgg
     2401 cgaccatcac gatccagccg agtccgttgg tcatcgttcc tcgcttctga atcggcagtg
     2461 cgattcggat tccttcatga cacgggattt tgcgcccgtg gggtccccgg gtggagaggg
     2521 cagaatcgat cgatgcccga cgaggccagg gaggccaagg cctaggggat tcgctcacct
     2581 tcctcgcatc gtcggagcaa gtcaaaggaa ttcgaagcag cgcggccggg agcagcacgc
     2641 ctcatgggtc ggcctctgaa tcggttagag aacattgctt ggtagacggt cgcgcccagt
     2701 gaccgcgagc aggcaggagt caggaatcgt ccgcgcattg cggacaccta agctccggcc
     2761 gaggcaacgg cgggccgggc ttcacgaagc cagatcaacg cactcctctc ggtctatcgg
     2821 gcaacgactg attttgtgct tccaagaaac tgattttcga cttcaagaat gccgagacaa
     2881 cctggtgtca gagaggtttc agcgattaga aagggcctag gaatgaatgg ctcttcatgg
     2941 aatcgtgtgg gtcgctcgca accgtggaat cgcgggaggc gttctgaagt cggcaagggt
     3001 ggtcgggaag cacatgggtc cctcctttga gaggaaccct cgctctacct cgtgtcagac
     3061 ccggtctcga gcggagctaa cgccataacc gcaagcgcac ttgcggcccg catgctggca
     3121 gcttcattcg tcaccgaccg accgatctca tcccccagat gggtggggcc tggagtccct
     3181 tcgaaccctc ggccggtgta gcgatcccga gaccattggg catcgcgctg atctgtctgc
     3241 taggtacctc gcccaaccgc gttgtcacga aagcctacgc cggcgcgaat ggtcgcccaa
     3301 caacagcggg cagcgacaac taattactag ggtccaatcg gcccgtggta ttctggcggc
     3361 gagcagctgc gtcccagcga tggtcgtcag tcgccgctgc taggtgctgt tgaagcgcat
     3421 tgcgttaggc agcgtagacg tgacacctag gagcgtcacc aaccaaagga gcattcgtga
//
LOCUS       DASRZF010000209         8260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3579928, whole genome shotgun sequence.
ACCESSION   DASRZF010000209 DASRZF010000000
VERSION     DASRZF010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 8260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8260
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3579928"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..773)
                     /locus_tag="VFG22_09300"
     CDS             complement(6..773)
                     /locus_tag="VFG22_09300"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HET6416479.1"
                     /translation="MMGMRIIQLLALSVLVGTLGLSGCKKGKDTSSAADEKHGPPPPS
                     GTMSFQGMYSYMADAGLFLDCASRQQWAVAHEGDNASLERAYADAKIAPGSPLLVTVE
                     GGLDFRPKVDGPGKETKLIIQRFVRTWPRETCGSVQPATLEDTTWALLDLRGKTITVQ
                     VNDKAPYLVLNSKKASAYGFGGCNRFFGAYETSGQSLSFGAMGATRMACPDGMDQEQE
                     FLTALNATKRYEIHGSKLLLFDGQTLVARFEARGPAE"
     gene            complement(844..1572)
                     /locus_tag="VFG22_09305"
     CDS             complement(844..1572)
                     /locus_tag="VFG22_09305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HET6416480.1"
                     /translation="MNIRAIVVDDEPLARDELRFMLGQCEAVEVVGEARNAPEAQGLC
                     NAEQPDVAFLDLRMPGPDGVALAETLMANHPQLKVVIVSAHDEGALRAFEARVADYLL
                     KPVRLERLRQAVERVRESVTGGSSSQEPLDRIAVRRKDAYIVLELSDVIYFEVKDELV
                     WAVVAEDRFAIDKTLAQLEAQLDPDEFFRSHRGFIVRYDRIRAIEPTGAGTYQLLLEH
                     PERPKIPLARERAKKLRERIPFSG"
     gene            complement(1569..2798)
                     /locus_tag="VFG22_09310"
     CDS             complement(1569..2798)
                     /locus_tag="VFG22_09310"
                     /inference="COORDINATES: protein motif:HMM:NF019314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET6416481.1"
                     /translation="MDIRLLIPELFEKLSLIATAGLLGVLVPPLRNRLLGVGQPRDIA
                     VVVIFGLLFSLWGSTMGVDWLGHHLNLRAIGIMIAGILGGPRAGALTGVLGGGFYVTR
                     VAPEAFPWALIASVLDGWLAGRMSVRREQMFIGWRVLGAAALVQLADLSVTAAGLSLS
                     GANPVRAIPSLAAQLGGVALGVALFMNVALVVIARQEQAVALVEAQSAANALSLTALR
                     SRLEPHFLFNALNTLRATIRRDPEQARALVSDLADLYRYLLHHPHDASVQNEVEHACA
                     YLAIEKARLGAERLAVQTSVDENVRDVRIPALLLQPIVENAVKHGITPKQGIGRITIR
                     ASDAGDRIRIEVEDQAEGPHQGPSTKGSGLALQTLRKRLDQQYEGRATLQLSSQDQGT
                     LVTVELPKDTDIIGELG"
     gene            2869..3060
                     /locus_tag="VFG22_09315"
     CDS             2869..3060
                     /locus_tag="VFG22_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416482.1"
                     /translation="MLRGVAWLCSYLLILGCGVNIKPKQVDQPCTRTTQCDTGLACEG
                     GVCVPSSDGGVDDGTDAGG"
     gene            complement(3064..3453)
                     /locus_tag="VFG22_09320"
     CDS             complement(3064..3453)
                     /locus_tag="VFG22_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HET6416483.1"
                     /translation="MKKLGGILGVIIGAVFVLAACEKSSSPMSFENLDPDFGGLQGGK
                     TVRVIGQSVRLDIGYAVYFGEFRSPQVSIENEKALLAVTPRRTEPGVVDVTIRADNGS
                     VFVVKRGFEYINQGGNLLDEAADPAAP"
     gene            3547..5916
                     /locus_tag="VFG22_09325"
     CDS             3547..5916
                     /locus_tag="VFG22_09325"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HET6416484.1"
                     /translation="MTKQNLLVVDADPRSLRVLEVSLRNAGYNVAGCPSVGKALEILH
                     ANKPDLILSDTTFSDMNGFDFVEQLRQNSEWSQIPFMFLSSDGSIESKIRGLELGVED
                     YLTKPIYIREVVARVGIELARQRRAGLAHKSSDARTRFSGSLSEMSVVDLLQTIDVSR
                     KSGVLTLVSTDGQEGMISFDSGAVINATVEDLVGEDAIYRHLLWHDGTFDLEFRRVSL
                     SERTVHRTTQALLMEGMRRLDEWSRLSELLPPFETVLEVDPAMLRERLREMPDDQNAM
                     VRLIDGTMTVSEVLRAHGGDHVEALRKVVDLYFEGMVREVGRIQDSIPILTEVSVRSS
                     APPLKEGVNTIPGPAGLPVAPLAVPAAPSVPTMTPLSRSAIVSELPAVFGPTSDHLTT
                     PVSLPAPDSQEDLELEEATTTPSGSPPKLELGWQPAPAPGSSRKVTPKGFQAVRPQSS
                     KYAGGTILNWAGRVPEIDTIEEVSGRETVEVGQWDRSLEDLREDPEVEEAIVAIDSDP
                     PPGPFVRSSPPGPPLEDQAEAVAFAAVRQSTPVPSYHEPPEVDPPPLTYEQVFNDTQS
                     AASLKAPRPVQPTSLTAKSSSASSMLPWALLILGVGFAFAAVVFLTSPWRGPQPAPSI
                     ELVAVGEPEPEMERAAATKPAPAPEPLAPKEPLDAEDATPVPVPEVIYAETGAIDTYD
                     AQLALARRLKRGSRAVAAYRRAIELNPQESPALAELGRLMLARNNTREAAELAERATA
                     IDPSNALAWVTLGAARQTRGDRQGARQAYQNCVKLGQGQYVSECRAMLR"
     gene            5991..7025
                     /locus_tag="VFG22_09330"
     CDS             5991..7025
                     /locus_tag="VFG22_09330"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6416485.1"
                     /translation="MNGRDPVGIAVIGAGGWGKNHVRNYAALPDADLRYICDRDEGIR
                     NAMAALYPSVEAVAELRTVLDDPSVKAIVVATHAPSHFEVAEAALREGRDVFVEKPLC
                     LSGEQAAALCALAERGGRILMVGHLLLYHPAVERLKGLIDDGELGEVLYVYAQRVNLG
                     VVRREENAWWSLAPHDISVANYLLGSSPEAVSATGSCYLQSERGIEDVVFATLHYPGG
                     RMAHVHVSWLDPHKTRKLTVVGDRKMAVFDDTSADQKLAVFDKGVEPPATLTYEEGVR
                     IRTGDILIPALKMAEPLRRECLAFLDAIRTRKAPVADGQSGLHVVRALEGGSASLAQG
                     GARIELSRGQ"
     gene            7025..7597
                     /locus_tag="VFG22_09335"
     CDS             7025..7597
                     /locus_tag="VFG22_09335"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET6416486.1"
                     /translation="MRAPYVHETAVVDQGANLGEGTRIWHFCHVSAGSKIGAGSVLGQ
                     NVFIGPGVSIGAGVKIQNNVSVYAGVTIADDVFLGPSCVFTNVLTPRAHVERKDEFAP
                     TLVGRGVTVGANATIVCGHALGEYALIGAGSVVTKDVRAHAVMVGNPARRRAWACRCG
                     EVLPKDGVCARCGDEYEVAAESCRWLRNRD"
     gene            7590..>8260
                     /locus_tag="VFG22_09340"
     CDS             7590..>8260
                     /locus_tag="VFG22_09340"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HET6416487.1"
                     /translation="MTKDEIPMLDVVAQNGPLIDRLAATAASVIRSGHYILGPEVAAF
                     EREVADYIGVQHAVGVSSGTDALLVALMALGIGPGDEVITTPFTFFATAGCITRVGAT
                     PVFVDIRPDTFNIDVEQVATAISERTRAILPVHLFGQSCDMEGLLELGRKHDLPIIED
                     AAQAIGTKSPVGPIGSLGAYGCFSFFPSKNLGGFGDAGLVTTQDAGLASKARVMRAHG
                     SQPKY"
BASE COUNT         1512 a         2566 c         2607 g         1575 t
ORIGIN      
        1 cggcttcact ccgcggggcc ccgtgcttcg aaccgtgcga ccagcgtctg gccgtcgaaa
       61 agcaagagct ttgacccgtg aatctcgtat cgcttcgtgg cattcagtgc ggtcaggaac
      121 tcttgctcct gatccatccc gtcagggcag gccatgcgag tcgcacccat cgcaccgaaa
      181 ctgagggact gccccgaggt ttcgtaggcg ccaaagaagc ggttgcaacc gccgaatcca
      241 taagcgctag ccttcttcga gttgagcacg aggtacggcg ccttgtcgtt cacttgcacc
      301 gtgatcgtct tgcccctgag atcgagcagg gcccaggtcg tgtcttcgag tgtggctggt
      361 tgcacgctcc cgcaggtctc cctcggccaa gtgcgcacga agcgctgaat gatcaacttg
      421 gtctccttcc ccgggccgtc aaccttgggc cgaaagtcca ggccgccctc caccgtgacg
      481 agcaatggcg agcccggggc gatcttcgcg tcggcatacg cgcgctcgag cgaagcgttg
      541 tccccttcgt gggcaaccgc ccattgctgc cgggacgcgc agtccagaaa gagtcccgca
      601 tcggccatgt aggagtacat tccctggaag ctcatcgttc ccgaaggcgg cggcggcccg
      661 tgcttctcgt cggcggcact tgaagtgtct ttgcccttct tgcagcccga aagcccgagc
      721 gttccgacca gcacactgag cgccagcagt tgaatgattc gcatccccat catgccacgg
      781 catgtaccac ttgagccggc aaaggctaaa ggatttcgat tacgggcccc gtcttatgag
      841 agctcacccc gaaaaaggga tgcgttcgcg cagcttcttt gctcgctcac gggccagcgg
      901 aatcttgggc cgctccggat gttctagcag cagctgatac gtgccggcgc ccgtgggctc
      961 gatggcgcga atccgatcat agcgaacgat gaaacctcgg tgggaccgaa agaattcatc
     1021 ggggtccagc tgggcttcca gttgagcgag cgtcttgtcg atcgcgaaac ggtcttccgc
     1081 gacgaccgcc catacgagct cgtctttgac ctcgaaatag atcacatcgg acagctcgag
     1141 aacgatgtac gcatcctttc ggcgaacggc gattctatcg agtggttctt ggctgctcga
     1201 gccgcccgtg acgctttcgc gcacacgctc gaccgcctgg cgcaatcgct ccagccggac
     1261 cggtttcaac aagtagtcgg caacccgggc ttcgaatgcg cgcagcgcgc cttcatcgtg
     1321 tgcggagacg atgacgactt tgagctgggg gtggttggcc atcagtgttt ccgcaagcgc
     1381 gacaccgtcc ggtccaggca ttcgcaaatc caggaatgcg acgtcgggct gttccgcgtt
     1441 gcagagccct tgcgcctcgg gagcgttgcg ggcctcgccg acgacctcca cagcctcaca
     1501 ctgtcccaac atgaaccgaa gctcgtcgcg agcaagaggt tcgtcgtcga cgacgattgc
     1561 gcgaatgttc atcccagctc tccaatgatg tcggtgtcct tcggcaactc cacggtcacc
     1621 agcgtaccct gatcctgcga ggaaagctga agcgtggcgc ggccctcgta ttgctggtcc
     1681 agtcgcttgc ggagcgtctg aagcgcgagc ccggagccct tggtcgatgg cccctgatgc
     1741 gggccctcgg cctggtcctc gacctcgatt cgaattcggt cgccggcatc agaggcccga
     1801 atcgtgatcc tcccgatccc ttgtttcggc gtaatcccgt gcttgaccgc gttttccacg
     1861 attggctgca gaagtagtgc agggattcgg acgtcgcgaa cgttttcgtc gacggaggtc
     1921 tgtaccgcaa ggcgctccgc tccaaggcgc gccttctcga tcgcgagata ggcgcaggcg
     1981 tgctctacct cattctgaac ggaagcgtcg tgcggatgat gcagtaggta ccgatatagg
     2041 tcggcgaggt cggagaccag cgcccgagcc tgctccgggt cccgccgaat ggttgcccgg
     2101 agcgtgttga gtgcattgaa caggaagtga ggctcgagtc ggctgcgcag cgcagtgagg
     2161 gacagcgcgt tggctgcgga ttgggcctct accagggcaa cagcttgttc ctggcgagcg
     2221 atgacgacca gcgcgacgtt catgaagagc gcgacgccca gcgcgacccc tccaagttga
     2281 gcggccagcg acggaattgc ccggaccggg tttgccccgg atagcgacaa gcctgccgcg
     2341 gtcacggaca gatctgcgag ttgcacgagg gcagcggcgc ccagcacgcg ccaaccgatg
     2401 aacatctgct cccggcgcac ggacatgcga ccagccaacc agccgtccag taccgatgcg
     2461 atcagcgccc acggaaaggc ctccggggcc acgcgcgtca cgtagaatcc gccaccgagg
     2521 acaccggtga gggcgcccgc acgaggtcct cccaagatgc ccgcgatcat gatgccgatg
     2581 gcgcgaaggt taagatgatg tccgagccaa tcgacgccca tggtcgagcc ccagagcgaa
     2641 aacagcagcc cgaaaattac gacgacagcg atgtcccttg gttgaccgac gcccaggaga
     2701 cggttgcgca gtggcggcac gagcacgccg agaaggcctg cggtcgcgat caggctgagc
     2761 ttctcgaaga gctccggtat caggagccgg atgtccacgt acggagccta gcagcgtttg
     2821 cgtcatcagg cgtggtgtca gatcgaaggc tgagctatcg tcgccgagat gcttcgcgga
     2881 gttgcctggc tgtgttctta cctgctgatc ctcggctgcg gtgtcaacat caaaccgaag
     2941 caagtcgacc agccatgcac gcgaacgacc cagtgtgaca ctggcttggc ttgcgagggt
     3001 ggggtttgcg ttccctcgtc cgatggggga gtcgacgacg gcaccgacgc cggcggctga
     3061 gcctcagggt gctgcagggt ccgcggcttc gtctaggaga ttgcctcctt gattgatgta
     3121 ctcgaagccc cgcttgacta cgaacaccga tccgttgtcg gcgcggatgg tcacgtccac
     3181 cacaccaggc tccgttcgcc gcggcgtcac ggccagcaag gctttctcgt tctcaatcga
     3241 cacctgggga gagcgaaact cgccaaagta gacggcgtag ccaatgtcca aacgtacgct
     3301 ttgcccgatg actcgtacgg tctttccgcc ttggagcccc ccgaaatccg ggtccaggtt
     3361 ctcaaaggac atcggtgagc tcgacttttc gcacgccgcc agcacgaata ctgcgccgat
     3421 gatcacgcct aggatgcccc cgagcttttt cattccaaca ataccaagtt atcacgttcg
     3481 tcgcgggaag gcatgcttga catgacactg tacccattcg cacactgccg ctgacaaaag
     3541 ggtgcagtga ccaagcagaa cctcctcgtc gtcgatgccg atccgcgcag cctccgcgtg
     3601 ctcgaagtga gccttcggaa cgcgggctat aacgtcgcgg gttgccccag cgtaggaaag
     3661 gcgttggaga tactccatgc caacaagccc gacctgattc tctctgacac gacgttcagc
     3721 gacatgaatg ggttcgattt cgtcgagcag ctccggcaga actcggaatg gtcgcagatc
     3781 ccattcatgt tcctttcgag tgacggttcc atcgagagca agattcgcgg gctcgagcta
     3841 ggcgtcgaag actatctcac gaagccgatt tacattcggg aggtcgtagc ccgggtcggc
     3901 atcgagctcg cgaggcagcg gagagccggt cttgcgcaca agagcagtga tgcacggact
     3961 cggttcagtg gctcgctgtc ggagatgagc gtcgtcgatt tgctccaaac gatcgacgtg
     4021 agccgcaagt ccggggtgct gacgctcgtg tcgaccgacg gtcaagaggg catgatctct
     4081 ttcgattccg gtgcggtcat caacgcgacc gtcgaggact tggtcgggga ggacgcgatc
     4141 tatcgtcatc tactctggca cgacgggacg tttgatctcg agttcaggcg cgtgagtctg
     4201 agcgaacgca cggtccaccg aaccacgcag gcgctcttga tggagggcat gcggcggctc
     4261 gacgaatgga gtcgtctttc cgagcttctt ccccccttcg aaacggtgct cgaggtcgac
     4321 cctgcaatgt tacgtgagcg acttcgcgag atgcccgacg atcagaacgc aatggtccgg
     4381 ctaatcgacg gaacgatgac cgtgagcgag gtgctcaggg cccacggagg cgatcacgtc
     4441 gaggcgcttc gcaaggtcgt cgacctctac ttcgagggca tggtgcgcga ggttggtcga
     4501 attcaggaca gcattccgat tctgacggag gtctccgtcc gatcgtctgc accgcctctc
     4561 aaagaggggg tcaacaccat ccccggcccc gctggcctgc ctgtcgcgcc actcgcggtg
     4621 cctgctgcgc cctcggttcc cacgatgacc ccgctctctc gttcggcgat cgtctcagag
     4681 ctgccggcgg tcttcgggcc gacgtcggat cacctcacga cgccggtttc acttcctgct
     4741 ccagactctc aggaggatct cgagctcgaa gaagcaacca cgactccgtc cggatcgcca
     4801 cccaaattgg agctcggatg gcagccggcg ccggcgcccg gctcttcgcg aaaggtaacg
     4861 ccgaagggtt ttcaggcggt gcgccctcag agctccaagt acgcgggtgg caccatcttg
     4921 aactgggccg gtcgcgttcc cgaaatcgac acgattgaag aggtttccgg gcgtgagacc
     4981 gtcgaagtcg ggcaatggga tcgctcgctc gaggatctcc gtgaagatcc ggaggtcgaa
     5041 gaggcgatcg tcgcgatcga ctccgaccca ccacccggtc cgttcgttcg gtccagtcca
     5101 ccgggtcccc cgctggagga ccaggcggaa gcagtcgcgt tcgctgccgt caggcagagc
     5161 actccggtcc cgagctacca cgagccaccg gaggtcgacc cgccgccact gacctacgag
     5221 caggtgttca acgacacgca gagcgctgcc agcctgaagg cgccgcgccc agttcagccg
     5281 acatcactca ctgccaagtc ttcctcggcg tcatcgatgt tgccttgggc gcttctgatt
     5341 ctgggcgttg gcttcgcctt tgcggcggtc gttttcctga cctcgccatg gcgggggccc
     5401 cagccagcgc catcgatcga gctggtggcc gtaggggaac cagagcccga gatggagcga
     5461 gcggcggcga ccaagccagc gccggcgccg gagcctttgg caccgaagga acccttggat
     5521 gcagaggatg cgactcctgt ccccgttccc gaggtcatct atgcagaaac cggtgctatc
     5581 gacacctacg acgcccagct cgctttggcg cgccgactga agcgaggctc gcgcgcggtc
     5641 gcagcgtaca ggcgtgcgat cgagctaaat cctcaggaaa gccctgcgct ggccgagttg
     5701 gggcgcctca tgttggcccg taacaatacg cgggaggcgg cagagctagc ggagcgggcc
     5761 acggcgatcg acccgagcaa tgccctggcc tgggtgacgc tcggggcggc acggcagacg
     5821 cgcggagatc ggcaaggggc gcggcaggcg taccaaaact gcgtcaagct tgggcagggt
     5881 cagtacgtct cggaatgtcg cgccatgctg cgctgacgcc gcggttccgg cacaagtttc
     5941 ggggatggcc gcccgtcctg ctaagctccg cggatgaagc agggggcggc gtgaacggaa
     6001 gagatccggt cgggatcgca gtcatcgggg cgggtggctg gggcaagaac cacgtgcgca
     6061 actatgccgc ccttccggat gcggacctcc gctacatttg cgatcgagac gaaggcatcc
     6121 gaaacgcgat ggccgcgctc tatccgagcg ttgaagccgt ggcggagctg cgcaccgttc
     6181 tcgatgatcc ctcggtcaaa gccatcgtcg tcgcgacaca cgcgccgtcg catttcgagg
     6241 tcgccgaggc tgccctgcgc gagggccgag acgtcttcgt ggagaagcct ctgtgccttt
     6301 cgggggaaca agcggcggcg ctttgtgcgc tcgcggaacg aggaggccgc atcctgatgg
     6361 tgggtcacct gctcctttat catcctgccg tcgagcgtct gaagggcctg atcgatgacg
     6421 gcgagttggg cgaagttctt tacgtttacg cgcagcgcgt caatttaggc gtcgttcgcc
     6481 gggaggaaaa cgcctggtgg tcgctcgctc cgcatgacat ctcggttgcg aactacctcc
     6541 tcggttcctc gccagaagcg gtgagtgcaa ccggctcctg ctatctccag tccgagcgcg
     6601 gaatcgaaga cgtcgtgttc gcgacgcttc actatccggg tggtcgcatg gcgcacgtgc
     6661 atgtgagctg gctcgatccc cacaagacgc gaaagctcac ggtcgtcgga gatcgaaaga
     6721 tggccgtctt cgatgatacc tccgctgatc agaagctggc tgtcttcgac aagggcgttg
     6781 aacctccagc taccctgacc tacgaagaag gcgttcgaat tcgaacgggt gacatcttga
     6841 taccagcact caagatggcc gagccactgc gccgcgagtg cctcgctttt ctcgatgcga
     6901 tacgtactcg gaaggccccc gtcgccgatg ggcagtcggg actgcacgta gttcgggcct
     6961 tggagggtgg ctctgcatca cttgcgcagg ggggcgcgcg catcgagctt tcgaggggcc
     7021 agtgatgcgt gctccctacg ttcacgaaac ggctgtggtc gaccagggcg cgaacctagg
     7081 cgaggggacg cgcatctggc atttctgcca cgtgtccgct ggttcgaaga tcggcgcggg
     7141 gtccgttctc ggccaaaacg tcttcatcgg acctggcgtg tcgatcggag ccggcgtgaa
     7201 gatccagaac aacgtttcgg tctatgccgg cgtgacgatc gccgatgatg ttttcctcgg
     7261 cccgagctgc gtatttacca acgttctcac cccccgtgcg catgtcgagc gtaaggatga
     7321 gttcgcgccc acgctggtcg gccggggcgt tacggtaggc gccaacgcaa ccatcgtctg
     7381 tgggcatgcg ctcggcgagt atgcgctcat cggagccggc agcgtggtca cgaaggacgt
     7441 tcgagctcac gccgtgatgg tcggcaaccc tgcgcggcgc cgggcctggg cctgtcgctg
     7501 cggtgaagtg ctgccgaagg acggtgtctg cgcccgatgc ggcgacgagt acgaagtggc
     7561 ggccgagagc tgccgttggt tgaggaatcg tgactaaaga tgaaattcca atgttggacg
     7621 tggtggccca gaacggtccg ctcatcgatc gattggccgc aactgcggct tctgtcattc
     7681 gttcagggca ctacattctc ggtccggaag tagccgcgtt cgagcgagaa gtcgccgact
     7741 acatcggagt ccaacacgcc gtcggcgttt cgagtggcac ggacgctttg ctcgtcgccc
     7801 tcatggcgct cggcattgga cccggcgacg aggtcatcac gacgccgttc accttcttcg
     7861 ccaccgccgg ctgcattacc cgcgtgggcg cgactccggt tttcgtggac attcgacccg
     7921 atacgttcaa catcgacgtc gagcaggtag ccaccgcaat cagtgagcga actcgcgcga
     7981 ttttgcccgt gcatctgttt ggtcagtctt gtgacatgga gggcttgctc gagctcgggc
     8041 gcaagcacga tctacccatc atcgaagatg ctgcgcaggc gatcgggacg aagagccccg
     8101 tcggtccgat cgggagcctc ggcgcctacg gttgtttttc gttctttccg tcgaagaatc
     8161 tcggaggctt tggtgacgcg ggattggtca ccactcagga cgccggcctc gcttccaagg
     8221 ctcgtgtcat gcgtgcgcat gggagccagc ccaagtacta
//
LOCUS       DASRZF010000210         4967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1866465, whole genome shotgun sequence.
ACCESSION   DASRZF010000210 DASRZF010000000
VERSION     DASRZF010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 4967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4967
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1866465"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="VFG22_09345"
     CDS             complement(<1..693)
                     /locus_tag="VFG22_09345"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET6416488.1"
                     /translation="MLRSLGIALLCLTAVASSRVKAEAPYVIVKRGDALSLIAERANI
                     SVEQLREWNRLDGDLIRIGQKLIIGQDARRPPPKAAKVHKTEPRSGNRLGSGSDDDGI
                     DWTPPFDYAAAAAEVEMEAEDEPTPRIAARDPETAVAVRSYDKPAKKKTLRRRYSVQS
                     GDTLAEIALRHDTTIADLLEANPGLDANRIHPGQTIDLGAPMPAVVFKLERGDTVLAA
                     ASRYGVSSRDLQR"
     gene            complement(805..2055)
                     /locus_tag="VFG22_09350"
     CDS             complement(805..2055)
                     /locus_tag="VFG22_09350"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6416489.1"
                     /translation="MRNSSAKDMASTDPDRRRRLSGGSIAPTRSTPEKIGRYELCFEL
                     ASGGMASVYLARAAGAPGFQKLVALKRIHAHLAKEKEYVEMFLDEARIASRITHANVC
                     SVFDFGEVNGEYFIAMEYLVGEPLSRVHRRVVANAEERSSPLLPSRMARIIAQAAEGL
                     HAAHELNDADGQSLNVVHRDVSAENLFVTYDGDTQVVDFGIAHARQRVHHTEAGQVKG
                     TFPYMAPEQMTAAVVDRRVDVWALGAVLWELLTLRRLFLRDTDVNTMYAVLSGEIRPP
                     SEYRSDVPPELDEIVLKALQRNPDERWQSAREMGKALRRFLAKQEELVGPAEIAEWMA
                     ELFPNGEARKHHLMEIARTSPDQIAADSPYQSELKSQRRNMAIPSTAKYRVPAPPPLP
                     ASLLVMVIGVATVLTLVALDWLRQ"
     gene            2215..2766
                     /locus_tag="VFG22_09355"
     CDS             2215..2766
                     /locus_tag="VFG22_09355"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HET6416490.1"
                     /translation="MNLGETYVLDEIDRQLLGELQSDCKRSLKEIGAAVGLSAPSIME
                     RVRKLEGAGIIKGYHALLEARKLGLDISAFIGVSISSPGLLSAFEDWVDSIAQVLECH
                     HVTGGHTLLLKVKTQNTKALEQLISRIRSMEGVASTETMVVLSTHTERVEIALPRGEV
                     ASDARRRKRPRRPADASKPIPTT"
     gene            2769..>4967
                     /locus_tag="VFG22_09360"
     CDS             2769..>4967
                     /locus_tag="VFG22_09360"
                     /inference="COORDINATES: protein motif:HMM:NF012531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HET6416491.1"
                     /translation="MQYGLPEKHGLYDPENEKDACGVGFVAQIKGVASHQIVKDANRI
                     LCNMDHRGARGSETNTGDGAGMLTAIPDKLLRIAAKAELGIDLPEPGKYGVGNIFLPT
                     NDEERAYCKAAFMKAIEAQGQRFLGWRVVPTDPDGANLGPTALESMPRIEQLFIGAAD
                     GMDQDSFERQLYVVRKSAASPLRVDETLEQRKMLYACSLSSRTIIYKGMLACEQVLPF
                     YADLTNPNYETHLAMVHSRFATNTFPTWERAQPLRCLSHNGEINTLRGNKNAMYSRQG
                     VVESELFGDDLKKLFPVAELGLSDSGTLDNVLEFLYMNGRSMPEAAMMMIPEAWQKHQ
                     QMPQVKRDFYEYHSCLMEPWDGPASIAFTDGKIIGATLDRNGLRPSRYYLTHDDRVIM
                     ASEVGVLPVPPETVKHKGRLQPGRMFLIDFEQGRMIPDEELKNDFATRRPYGEWLREQ
                     RLELSKLPVHGDAHGFEPETLLARMQSFGYTTETMQFMLRPMIEEKRDPVGSMGNDSA
                     LAVLSDQPRLAYDYFKQLFAQVTNPPIDSIREELIMGVECYVGPERNLLTTTPEHAFR
                     LRIPHPILTNEEVRALKTIDERGWRSKLIDITWARSEGPEGLELTLQRIRQEAEQAVD
                     EGYSLILLTDRGAGQDRIPVSALLATGAVHHHLVATEKRTRVGIIVATGEAREVHHHC
                     CLLGYGADAINPYLAFEALWQERRLGTLDAERFPDDDSIVDGYRKAVAKGL"
BASE COUNT          987 a         1562 c         1479 g          939 t
ORIGIN      
        1 gcgttgaaga tctcgcgagc taacgccgta gcgactcgca gctgctagga cggtgtcccc
       61 acgctcgagc ttgaagacca ccgctggcat cggcgctccc aagtcgatcg tctggccagg
      121 gtggattcgg ttcgcgtcta atccggggtt ggcctcgagc agatccgcaa ttgtcgtatc
      181 gtgcctcaga gcgatttctg caagcgtgtc gccgctctgc acactgtagc gtctacgcag
      241 ggtcttcttc tttgccggtt tgtcgtaact ccgtacggcg accgcagttt cggggtcgcg
      301 ggctgcaatt cgcggagtgg gttcatcctc agcctccatc tcgacttcgg cggccgccgc
      361 tgcgtagtcg aatggagggg tccaatcgat gccgtcgtca tcggagccag aacctagccg
      421 attccccgag cgcggttcgg tcttgtgcac tttcgcagct ttgggcggag gacgtcgagc
      481 atcctgtccg atgatcagtt tttgtccgat gcgaatcaga tcgccatcca accgattcca
      541 ttcgcgcagt tgctcgaccg atatattcgc gcgttccgca atcaagctca gcgcgtcgcc
      601 tcgtttcacg atcacgtaag gagcttcggc cttcactcgg gacgaagcca ccgcggtgag
      661 gcacaagagt gcgatcccga gcgaacggag catgcgagat cggtgtatcg agaatcgctc
      721 ttttcgccaa gaaagaagcg agatgcgaga aattttcgct cgaacgagga ctgatcattt
      781 gtagagatgc ttggctcgat gacgttactg gcgaagccag tcgagcgcca ccagcgtgag
      841 caccgtcgcc acaccgatca ccattaccag cagactggcg ggcagtggcg gaggcgctgg
      901 cacgcgatac ttcgccgtgg acggaatcgc catgttccgc cgctgactct tgagctcgga
      961 ttggtacgga gaatctgcgg cgatctggtc tgggctcgta cgcgcgatct ccatgagatg
     1021 atgcttgcgc gcctccccgt ttggaaacaa ctcggccatc cattcagcga tttccgccgg
     1081 gcccactagc tcctcctgct tcgcgagaaa acgtcggagg gctttcccca tctctcgagc
     1141 gctttgccaa cgttcgtcgg gatttcgctg cagcgctttc aatacgatct cgtcgagctc
     1201 cgggggcacg tcactcctat attcagaagg cggccgaatc tcgccggaca gaaccgcgta
     1261 catcgtgttt acgtccgtgt cccggaggaa cagtcgtcgc agtgtgagca gctcccaaag
     1321 gacggcgccg agcgcccaaa catcgactcg tcggtccacc accgccgcag tcatctgctc
     1381 gggtgccatg taagggaacg tgcccttgac ctgtcccgct tcggtgtggt gcactcgttg
     1441 tcgcgcgtgc gcgattccga aatccacgac ctgggtgtcg ccatcgtaag tgacaaatag
     1501 gttctcggca gacacgtccc ggtgcacgac gttgagcgat tggccgtcgg cgtcgttgag
     1561 ttcgtgcgcg gcatgaaggc cctcggcggc ttgcgcgatg attcgagcca ttcgcgaagg
     1621 aaggagtggc gagctgcgtt cctctgcgtt cgccaccacc cgacgatgaa cgcgcgacag
     1681 aggctctcca actaggtact ccattgcgat gaagtactct ccattgactt ctccgaagtc
     1741 gaagacactg caaacgttgg cgtgcgtgat ccgcgaggcg atgcgcgcct cgtcgaggaa
     1801 catctccacg tactccttct ccttggcgag atgggcgtgg atgcgcttga gcgcgacgag
     1861 cttctggaat ccgggcgccc ccgccgcgcg cgcgagatac acgctcgcca tgcctccgga
     1921 cgcaagctcg aagcagagct cataccgtcc gatcttctcg ggcgtgctgc gcgtcggagc
     1981 gatcgagcct cccgagagcc gccgccggcg gtctgggtct gtagatgcca tgtccttggc
     2041 gctcgaattc ctcatcagga gtttctctgg ttaggagcag gaacgcccct tagccgtcta
     2101 catcaaagta ttacacaaat aacgcagttc acagggatat ttcgaccaga atcgctcggt
     2161 ttgactttca gggatgtgca ccctatctat taggccccgt aaaggattca cctaatgaac
     2221 ttaggagaaa catacgttct ggatgaaatt gatcggcagc tcctgggcga gcttcagagc
     2281 gattgcaagc gctctctgaa agagatcggc gctgccgtgg gtctcagcgc cccgtcgata
     2341 atggaacgtg tccggaagct cgaaggcgcc gggatcataa agggatatca cgcgctcctc
     2401 gaggctcgga aactgggcct cgacatttcc gcgttcatcg gggtctccat cagcagcccc
     2461 gggctgcttt cagccttcga ggactgggtc gattcgatcg cgcaggtcct cgaatgccac
     2521 cacgtcacgg gtggccacac cctgcttctc aaggtaaaga cgcagaatac gaaggccctg
     2581 gagcagctca tcagccgtat caggtccatg gaaggggtcg cgagcaccga gaccatggtg
     2641 gtgctttcga cccacaccga gcgggtagag atcgcactac cccgtggcga ggtcgcgtcc
     2701 gacgcgcgcc gacgtaaacg cccccgccgc ccggcggacg ccagcaaacc catacccacc
     2761 acgtgaacat gcagtacgga cttccagaaa aacacggtct ctatgacccg gaaaacgaaa
     2821 aggacgcgtg cggcgtcggc ttcgttgctc agatcaaggg cgtggcctct caccagatcg
     2881 tgaaggacgc caatcgcatt ctctgcaaca tggaccaccg tggtgcgcgt ggctcggaga
     2941 cgaacacggg cgacggcgct ggaatgctca ccgcgatccc cgacaagctt ctgcgcatcg
     3001 ccgccaaggc ggagctcggc atcgaccttc cggagcccgg caagtatggc gtcggcaaca
     3061 tcttcttgcc gaccaacgac gaggagcgcg cctattgcaa ggcagccttc atgaaagcca
     3121 tcgaggctca gggacagcgc ttcctcggct ggcgcgtcgt acccaccgac ccggacggcg
     3181 ccaatcttgg acccaccgcg ctcgaaagca tgccgaggat cgagcaactg ttcatcggcg
     3241 ccgcggatgg gatggaccaa gattcgttcg agcgacagct ctatgtggtc cgtaagagtg
     3301 cagcgagccc gcttcgcgtc gatgaaaccc tcgagcaacg caagatgctc tacgcgtgct
     3361 ctctctcgag ccgcacgatc atctacaagg gaatgctcgc gtgcgagcag gttctgccgt
     3421 tttacgcgga cctcacgaac ccgaactacg aaacccacct cgcgatggtg cattcgcgct
     3481 tcgcgacgaa caccttcccg acctgggagc gcgcgcagcc actgcgctgc ctcagtcaca
     3541 atggcgagat caacaccctt cgcggcaaca agaacgcgat gtattcgcgt caaggcgtcg
     3601 tagagagcga gctattcggc gacgatctca aaaagctttt tccggtcgcc gagctggggc
     3661 tctcagactc gggcaccctc gataacgtcc tcgagttcct gtacatgaac gggcgctcga
     3721 tgcccgaagc cgcgatgatg atgattcccg aggcctggca gaagcaccag cagatgcctc
     3781 aggtgaagcg ggacttctac gagtaccact cctgtctgat ggagccctgg gacggccctg
     3841 cctcgatcgc gttcaccgac ggaaagatca tcggcgccac gctggaccgc aatggtctgc
     3901 gcccgagccg ctactatctc acccacgatg atcgagtgat catggcgtcg gaagtcggcg
     3961 tgctccccgt cccacccgag acggtgaagc acaagggccg cctacagccc gggcgcatgt
     4021 tcctgatcga cttcgagcag ggccgcatga ttccggacga ggagctcaag aacgacttcg
     4081 caacgcgtcg tccctacggc gaatggctca gggagcagcg tctcgagctc agcaagctac
     4141 ccgtacatgg cgatgcgcac ggcttcgagc ccgagacact tctcgcgcgc atgcagtcgt
     4201 tcgggtacac gaccgaaacg atgcagttca tgttgcgccc gatgatcgag gagaagcgcg
     4261 atccggtcgg ttccatgggc aacgactcgg cgctcgcggt cctgagcgat caaccgcgcc
     4321 tcgcgtacga ctatttcaag cagctctttg cgcaggtcac caacccgccg atcgactcga
     4381 tccgggagga actgatcatg ggagtcgagt gctacgtcgg gcccgagcgg aacctgctca
     4441 ccacgacgcc cgagcacgcg ttccgccttc gtattccgca cccgatcttg accaatgagg
     4501 aggtcagggc cctgaagacc atcgacgagc gcggctggcg cagcaagctc atcgacatca
     4561 cttgggctcg aagcgagggc cccgaggggc tcgagctcac gctccaacgc attcgccaag
     4621 aagctgagca ggcggtggac gaggggtaca gcctcatcct gctgaccgac cgtggcgcag
     4681 gccaggaccg cattccggtc agtgcgctgc tggccaccgg cgcagtccac catcacctcg
     4741 ttgcgaccga aaagcgcacg agagtcggaa tcatcgttgc aaccggcgag gcgcgcgaag
     4801 tgcaccatca ttgctgcctc ctcggatacg gggcggacgc gatcaacccc tatctcgcgt
     4861 tcgaggccct ctggcaagag cgccgcctgg ggacgctcga cgcggagcgg ttccccgacg
     4921 acgacagcat cgtcgatggt tatcgcaaag cggtggccaa gggcttg
//
LOCUS       DASRZF010000211        34200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4274009, whole genome shotgun sequence.
ACCESSION   DASRZF010000211 DASRZF010000000
VERSION     DASRZF010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 34200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34200
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4274009"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VFG22_09365"
     CDS             complement(<1..467)
                     /locus_tag="VFG22_09365"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF013398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HET6416492.1"
                     /translation="MKLTDWLTEHITDDPRASRWFDSPEAEGRIARCYKDLLSGYEID
                     PSTILKTTRKLGADEARGRVEVREIAFYSICAHHFLPFFGHVSITYVPGDRILGLGKL
                     PRLVEAYARRFQIQENLVRDIAREMMESGHARGVRVQSEARHLCMISRGPQSAA"
     gene            complement(464..1108)
                     /locus_tag="VFG22_09370"
     CDS             complement(464..1108)
                     /locus_tag="VFG22_09370"
                     /inference="COORDINATES: protein motif:HMM:NF021998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase CarF family protein"
                     /protein_id="HET6416493.1"
                     /translation="MLQFLRRAVLAASIFMAVILTAWVGLSVGRFMSEGWWLAVLIAL
                     PLGIAAIDLLTGIVHWACDRFGEASTPVLGPLLIRAFREHHVDPRQMVEHDWIETNGE
                     PCFFSALALLALAFVAPSVQSGLVAALVTIVWTMAAVGCWANQVHKWAHMSPAPRWAR
                     LLQHAGLALRPNAHACHHQAAHDSGYCISTGWMNPLLDRLGIWSWLERSLRRTP"
     gene            1257..1664
                     /locus_tag="VFG22_09375"
     CDS             1257..1664
                     /locus_tag="VFG22_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416494.1"
                     /translation="MFVPKKMIPRLAAAALALTGCGDGGGTAGTGGTAGTGGTAGAGG
                     TAGTGGIGGALAESLEAFCVKLVDCFPGVYGSTQECTEYLTTYYGISGGTSATCEAAL
                     VSYLDCGTQLSCEELTTSNSCDDEFEAAYNACT"
     gene            complement(1737..2042)
                     /locus_tag="VFG22_09380"
     CDS             complement(1737..2042)
                     /locus_tag="VFG22_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416495.1"
                     /translation="MAHALVGLRSVLRSRLADENVFGVQVDHGCLSIGRWRRRLRRRY
                     AASLYAIEQLIEKGWALFSLEEIGPALDSEIAKLHRLESLENQAQLALSSTDIGVGD"
     gene            complement(2044..2250)
                     /locus_tag="VFG22_09385"
     CDS             complement(2044..2250)
                     /locus_tag="VFG22_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416496.1"
                     /translation="MMFWQCNECGGLLDAVPRSSVCPECGTASSQIMRAAESDSDLSE
                     LRDLWVHIGMEQDAPLLDFSGTQG"
     gene            2539..3024
                     /locus_tag="VFG22_09390"
     CDS             2539..3024
                     /locus_tag="VFG22_09390"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET6416497.1"
                     /translation="MLTRWNDIGFGGLNRELLSLNDLRTRMDRLFLDFERGWGTGHDP
                     EARQAIAMGSAWPRIVLYDAGSELRLRAELPGMSESDLDITVEQSSLTVRGARETQVP
                     EGYSVHRQERGNRSFARSFSLPCRIDAEKTRANLKNGILDMTLPKVPEERPRQIRVQV
                     S"
     gene            3050..3427
                     /locus_tag="VFG22_09395"
     CDS             3050..3427
                     /locus_tag="VFG22_09395"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET6416498.1"
                     /translation="MNSETTSLTQRQAPNPERPEQAELISITPAVDIYENQDEILVVA
                     DFPGVPKDSLSIELDRSELLIEGAQAPVEGGPQPIVFSRAFRVPNTVDPDGVTAELTQ
                     GVLKVHLAKSEAAKPKRIEVRGG"
     gene            3535..4248
                     /locus_tag="VFG22_09400"
     CDS             3535..4248
                     /locus_tag="VFG22_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HET6416499.1"
                     /translation="MELLDPSAARPPPIPTSIEVIYRDDAVVVVDKPSGLSVHRGDDQ
                     GSTFALNLTRDAIGQWVYPVHRLDRATSGVLVFALSSEHARALQESLDQRSFHKTYLA
                     LVRGTPPERGVIDSPMAKREGGPDVESITEYELLFVSDRRLSLVEARPRTGRRHQIRR
                     HLRRIDHPIAGDVNYGNGADNRRYRAEVGLYRLALHAKRLSFLHPASGERVTFESPVP
                     EDLAEPLRRIGVPETLLGT"
     gene            complement(4428..6005)
                     /locus_tag="VFG22_09405"
     CDS             complement(4428..6005)
                     /locus_tag="VFG22_09405"
                     /inference="COORDINATES: protein motif:HMM:NF023649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HET6416500.1"
                     /translation="MRRIKPALWTLFALAMAVIGCKPPPPTTPAAIASEPNRLYWGDT
                     HVHSSYSSDAYAMGNHTADPDTAYRWAKGLPVVHPRTQAKVQIEEPLDFMVLTDHAED
                     LGRHLDLDPASAWGEIVEAAERNYAPCSFTTFIGWEWTSAPDGKSLHRIVLMKDAAEQ
                     ANQLEPFSALHSARPEDLWSWLEETSARVETDFLAIPHNSNLSKGAMFAKVDSEGQPI
                     TAAYAASRMRWEPLAEVSEGDYARSGLLRGLAIGQAVGANPFELGLLGSTNSHTGLAS
                     AEEDNFWGAAASESETGDRPQSGTEGIRGAELSAQGLTAVWAEENTRASIFEAFKRKE
                     VYATTGPRIRVRFFGGWGFNKKQAGDPRMVTLGYTLGWPMGSKLKKAPEDKAPGFLMY
                     AVKDPDGANLDRIQIVKGWVDADGKTHERIYDVVWSGKREPGPDGKVPPVTDTVDSAT
                     AQYLNSVGAPSLRGFWADPDFDSTQRAFYYVRVLQIPTPRHTLYDAVARGIDPKTLKQ
                     PLTIQERAYTSPIWYTP"
     gene            complement(6012..6509)
                     /locus_tag="VFG22_09410"
     CDS             complement(6012..6509)
                     /locus_tag="VFG22_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416501.1"
                     /translation="MLMAGLLTLLNAMGASPTPQKMENYKLDYRERMHHVHMDFSGTR
                     EKSTSGRREKTMTIGIGYEYHIDRQFNGVSFEVLGQKLGKFFGKGPQDWWVGGGIGWW
                     PIRHVKVFMQAGALFDDLGTSTTGRVGVGYNLNFFMLAVMPYVYVQTTDNAQFTWNIG
                     ARIQY"
     gene            complement(6516..6977)
                     /locus_tag="VFG22_09415"
     CDS             complement(6516..6977)
                     /locus_tag="VFG22_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416502.1"
                     /translation="MNVASILFVSLLLLPATCMLACGGQGEVRKTGGTYAGRGPDCDY
                     RVIRNPIAEPVEPYEEIGVIDIDAFGAQQLPNDEERFRKVVGPKVCALGGDGVIPAIN
                     IYGRWVHGTVIRFNPAQCDRCNAEVHGSPAIVDRPSVEDIPVIKDVKGEEG"
     gene            7104..7754
                     /locus_tag="VFG22_09420"
     CDS             7104..7754
                     /locus_tag="VFG22_09420"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HET6416503.1"
                     /translation="MEPLPRFVPLDRTIAFSDGVFAVVITVLVLGIEIPSDAVLTGAA
                     LAVERMKLLHQLLIYVVAFWMVGMYWSQHSLFFAGLKQIDRGIVVLNLLFLLLVTLLP
                     FVTQLMGAKRHDWKVVLVFGVVNLFATLVLERMWSRIAAMPEAHKSPQTAMLAARIRI
                     GVRVYAGVMVLGVLVASLDVRAGTFVFILIPIAHFYNYLRDPFRSRFGGVSSGANK"
     gene            7760..9316
                     /locus_tag="VFG22_09425"
     CDS             7760..9316
                     /locus_tag="VFG22_09425"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HET6416504.1"
                     /translation="MPILAIDLGTSNVKAAVVDRKGTLLGTGRGSIETLHTADGGAEQ
                     DPELVWEAVLNATDQALASLANRRDVRGVACASQYSSIVPVDAAGSPTGNMVVWMDKR
                     GAPERLRQLPGGTQMKPSLLQMARWLQIHGIPPLASGSDSLAHMRWIKLARPEVYGRT
                     ATFLEPMDYVTARLSGRRVTNLCSSFMMLLTDNRRPNPAYHPTLLKWSGLDIEKLPEL
                     VPIDEPLGPILPAIAERLGLAPDTLVFPPLNDTQAGGIGASAFAGEHAGVSIGTTGVC
                     ITHVDFKKTDVLNSLASMPSPFPGRNFVMAEAGIGGGAVKYFLEHLVFARDRFGDHSL
                     EERFSALERAIEPIEPGSDGVLFLPWLTGSITPAEDGKVRGGFLNISLRTTREHLAKA
                     VLEGVAFNQRWVQERVERFAKRDISRIKFYGGGALSSAWSQIFADVLNCPVEQLRDPE
                     FAVSRGVALLGFHRLGELALDEIEAGVPVAAVYQPRPEASVRYDAMFTQFVRVFKKNR
                     DVFRALNVGS"
     gene            9319..10743
                     /locus_tag="VFG22_09430"
     CDS             9319..10743
                     /locus_tag="VFG22_09430"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HET6416505.1"
                     /translation="MSDTIEDLMRGLYPYAESHGVIRAFPEQGRSTDAILDELRAIAS
                     QEDQAWENGRCSGTMYSGDHEHYDFLNAVFSLFSYVNSLQRDICPSMTRFESDIIAMT
                     LDLMHGEEVRKQNPAQKAVGAVGFGGTESIINPLLIYRDKARAERGIDRPKVIVPDTA
                     HPAFRKGAHLLGIEVVDAPTHPKTTEVDLDFVRDRLDARTVAIVGSAGNYPYGTIDPI
                     EALSELALEHGVGLHVDGCLGGFILPWGQRLGYEIPVFDFRLPGVTSISADTHKFGYG
                     LKGTSVVVYRDASFRKYQYHIEPMWKGGIYVSNGLAGSRSGGLIAATWAAMVRYGKEG
                     YLRYAKQIFETSFRMQDSVKSHPDLSIMGTPTFCFSFRSEEFNIYHVNDFMKTRGWRF
                     NGQQHPAAIHMCVTRPQTRSGIAEAFETDLAEAVAYARKHRDAEPKSSAIYGGGATGL
                     PTDSAEGIELFMAMALDTLQSYPF"
     gene            10812..11681
                     /locus_tag="VFG22_09435"
     CDS             10812..11681
                     /locus_tag="VFG22_09435"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET6416506.1"
                     /translation="MTRIYILAFTGIAVIAALDYWTGHEISFAVFYLLPVALLAWRTS
                     LRTAIAAAVLSAVLWYSADVSSHEYSHPAIGVWNAIARSIIFIFAAVALDRVRTLLER
                     ERALARTDPQTGLYNLRAFEELLEHELSRTQRTGRAFTLAYMDLDRFKAVNDRFGHAA
                     GDLVLREVGTKLATTLRGIDATARLGGDEFAILLSETDEAQAVLALARIQRAIALTLR
                     SIRAASEIGVGASIGAVVYSSGTATSGDLLGEADALMYRAKQERPGSIRIESPEAWRT
                     STSPAAVMTRSEP"
     gene            11713..13095
                     /gene="mgtE"
                     /locus_tag="VFG22_09440"
     CDS             11713..13095
                     /gene="mgtE"
                     /locus_tag="VFG22_09440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HET6416507.1"
                     /translation="MTQTAGSAEDQAPPWETLQRLIDAQDRPAAQAFVQGVPAGDLPR
                     LLSLLGNEESARLLEMLEPTTSGFLMESIPEAQAAEVLGELDSDKAAEIVAHLPSNEQ
                     ADLIASLPDDEAQAILEQMPPEDAADAERLAQYPEGTAGALMITEYLSFPHDVTVGAV
                     LQDLRQNTERYQRHDVQYIYVTDAAGILRGVLRLRDLVLTPERTPVEQIMITAMEAVP
                     VQAPLATLRDFFAARSYRGVPVTDRAGRLVGVVRHADVAEAVGEREVSDHLKAQGIVG
                     GEELRTMPTLTRAWRRLSWLSINILLNIMAASVIAFYQETLAAVIVLAVFLPIISDMS
                     GCSGNQAIAVSMREITLGLIRPSDALRVWSKELAVGLINSVALGTLLGLAAYFWQGNI
                     YLSLVVGLALAVNTIVSVSLGGLVPLFLRGMRFDPALASGPILTTVTDMCGFFLVLSF
                     ASAMLPLIGG"
     gene            13147..14274
                     /locus_tag="VFG22_09445"
     CDS             13147..14274
                     /locus_tag="VFG22_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416508.1"
                     /translation="MPSVIPTRIAPSVAPADLSPAKVSRSFHQLVESGAKIRAAGEAK
                     SDPMLLLSSGYTPKYRIDLFDTHFYFSNVLQNPALRFFVAYVVQKRPTTGRTEIFPRI
                     FYKDLSLVWRCASHVIATDGDFWIGKGDVKVLARGGFEVTECVESTTDLPFEMQAALE
                     TLNRKTRHARMDEEAIYLVLRSAPSSRTAPYRDFTEPRRKAAADRRNLINGGRSIARF
                     TRKNDPTSLQIVDGFVPDFTKGVVEVSQIKSAMYGGMLERFRILSRNRKIQYLFTAGP
                     KHVWIIPPQALTTELSSYGVRTVDVVADEDLFVPGYEYHYFDESADESEHFSQIPEGF
                     AGELSEHDNDRADASAWLDQIPVIREFRRKVMGRKHKPFRF"
     gene            complement(14365..14937)
                     /locus_tag="VFG22_09450"
     CDS             complement(14365..14937)
                     /locus_tag="VFG22_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416509.1"
                     /translation="MKPTLLLASLGLLACASTESPGDAGIDSGLDASVLERRIFVTAS
                     VQNADFGGLDGADALCASQASAAGLDGEFKAWLSTVVSSVSDRLTQSSEPYVLVDGTP
                     VANDWDDLVDGSILVPINLDASGQLRGGDVWTGTRPNGLPYTQGDCSGFTNGTDGIGL
                     CGTTASAGANWTANDTPKCSLGLRLYCIEQ"
     gene            15101..15670
                     /locus_tag="VFG22_09455"
     CDS             15101..15670
                     /locus_tag="VFG22_09455"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HET6416510.1"
                     /translation="MAVDSSIVEWSLPSGYAASSVEARDTGFRGMGLFARRLIREGET
                     IVAFGGTVIDRAGLLGLPRSAIRHTLQIDEELFLVSDRPGPADYVNHSCQPNAGLCGQ
                     VVLVAMRTISRDEEITYDYAMSDACAYDEFPCSCGSIQCRGAVSGSDWARPDLWKRYP
                     GFFSPYIQSQIEGLRRLSRRTGRKVRSIR"
     gene            15667..17277
                     /locus_tag="VFG22_09460"
     CDS             15667..17277
                     /locus_tag="VFG22_09460"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6416511.1"
                     /translation="MSPPNEDGGAPAAKQTGKASGVKVRRATLEDIPGIYACEIAAYP
                     QIPPHTLPAARELQFEIEAFPDGQLVAVSGDTVVGYASSLIVQLDDDSPWYSWGEITG
                     NGTFSTHTPSGDTLYGADIAVHPDFRGRGISKKLYQGRKRILRRFNLRRMVAGGRIPG
                     YQEHAGSITAEEYVARVIRGELRDPSLNAHLHAGYHVNSVHMGYARDEQSLNYATFLE
                     YLNPNFSPERRSIAGAPLKRPVRRIRVCAAQYEMRRISSWEEFEQQVDFFVATAEQYN
                     SHFLCFPELFTAQLFSMMPPTMQPLEAVRELASMADRYKEMFVKRAKKSGIFIIGGSH
                     PVVVDGKMLNVAHLFTPSGNVYTQSKLHITPNERAEYGIQPGDGLHIFDTGHARIAIL
                     VCYDVEFPELARILTLAGAEVLFVPFSTDERKAYLRVRYSSQARAVENMVYVVLSGNV
                     GNLPQVDNFLINYGEAIICTPSDFAFPPDAIAAKADFNTETVVISDLDLGALEQVREL
                     GSVRPLRDRRPDLYHIAPREPVKIIRTR"
     gene            17474..17926
                     /locus_tag="VFG22_09465"
     CDS             17474..17926
                     /locus_tag="VFG22_09465"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET6416512.1"
                     /translation="MSESADNGLSASEQLLEIEAIKQLKARYFRLMDSKEWLPFSQVF
                     TDDVVVDVSQDKHAPGDTIEGRVNVVDFIRKAVGKATTVHHGHMPEIEILSPTEARGV
                     WAMFDYVEFRPSRGIRGYGHYHEEYRKERGRWHICKLKLTRLRVDPLG"
     gene            18012..19913
                     /locus_tag="VFG22_09470"
     CDS             18012..19913
                     /locus_tag="VFG22_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET6416513.1"
                     /translation="MPILVAAGLSKAYGETRLLDGVDLSIHEGERIGLVGNNGSGKST
                     LARILVGEEPPDEGRVARRREASIRYLPQEPTLPAGRSAREVVLEGLGAWAEAHARHE
                     KLSRLIAAQEQGWEAYSEPQAAAAQEVERLGGWENSLRADVLIQQLGLHDIERSVDEM
                     SGGERRRVALARLLVEQPALAILDEPTNHLDADTIEWLETYLREQYRGALVLITHDRY
                     VLDRVVTRTLEVDQGQVYSYEGGWHAYLEAKAERLAHANRAEANRQNLLRTELEWLRR
                     SPKARTTKSKSRVDRVEALRDQTAPKGEQVAQFAAQTQRLGNTVLEAQSLGVSIGTLE
                     LVESLDLILSRGDRLGIVGPNGAGKTTLLRVLLGELEPASGRVVVGANTKFAYFDQGR
                     SGLSEDDSVQQNVAPDRPQVTFQGRSMDIRSYLKRFLFAPHRTRDKVSSLSGGERARV
                     ALAKLLLVPANVLVLDEPTNDLDVSTLSALEEMLVESNSTALIVSHDRYFLDRVANKI
                     LAFEGGCRVVAYAGNYSDYREQRNAAQIDGKRGTEKGPKAPKTSSAVKVQKLSYKEKQ
                     ELESIVGEIERAEREAAEAEALLNDPMLYADRAAQVPAIVARQAELRAQVDRLMSRWI
                     ELEAKSNPG"
     gene            complement(19910..21256)
                     /locus_tag="VFG22_09475"
     CDS             complement(19910..21256)
                     /locus_tag="VFG22_09475"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET6416514.1"
                     /translation="MFRRPSKTTNLRTQLGNALKAERLNEALDLYELIEKQTPDEPRW
                     SHRRGDLLQRMGRKADAVLAYERAVDLYAAKGFVARAAAMAKLILAIDPSKGEVLERV
                     DPQAARRLHRQNRALIVTADDSDVEDDDGTPTDTQSLITDALPLIADRRAPDGETRFM
                     KPEKTEDITLDLTNVELLERPKPSDALVSQRPSAATLAQLPSMPLFAEVPRGLLQTMV
                     RESGLVDVEDGQRLTTAGTSADALYVLVEGNAVGRRGSDMQSMLLGEGDVAGVSCLLS
                     NVSYGEEVVACGRVRALRIGKDLLDRLVARHPPFGDVLLEVLSRRLIATLLRTNPIFT
                     AFDEVTRKQLARLFEVRRAHAGTRIVEAGKRSDAMYLPLHGRLVVRTADGRRLGNMDL
                     GYPIGQESLLTREASRVTIEAASEVLILRMPVPRFSELLMKRPDIVQRFQMLQLRA"
     gene            21443..22174
                     /locus_tag="VFG22_09480"
     CDS             21443..22174
                     /locus_tag="VFG22_09480"
                     /inference="COORDINATES: protein motif:HMM:NF004858.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HET6416515.1"
                     /translation="MTEIVRYERSGPVGTITMDDGKVNCMSLAMLSELNRALDQAQAD
                     GVALVITGREGMFSGGFDLATFKQGGEALYQMLKGGAELVERVMSFPRPIVTAAGGHT
                     IAMGAFLAIGADARIGAEGAFKIVCNEVAIGLTLPRFAIEIIRHRLTPAHFNRALVLA
                     APYSPKEAVEAGFFDRVVPPSEVLTEATNLAQALSQLNAKAHAESKLLVRERALNALR
                     EAIESELGSFQIFADTIGSGMKVSR"
     gene            complement(22193..22702)
                     /gene="greB"
                     /locus_tag="VFG22_09485"
     CDS             complement(22193..22702)
                     /gene="greB"
                     /locus_tag="VFG22_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HET6416516.1"
                     /translation="MSRQRTRPDTSGDPGYITPEGYRRFEEEADRLWNVDRPKMAKAV
                     QVAAAEGDRSENAEYQYSKQRLAAIDRRLRFLGRRLKVLTIVDEKPPDDGRVYFGSWV
                     TIEHPNGQSETYRIVGPDEIDAKKKWISMDSPMGRSLLSREVGDEITVRRPKGEVTCE
                     IIEVRNGDG"
     gene            complement(22759..24429)
                     /locus_tag="VFG22_09490"
     CDS             complement(22759..24429)
                     /locus_tag="VFG22_09490"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET6416517.1"
                     /translation="MAEPQLVLDDAYRWEKTVPNQLWMTQPIGNGQVQTYTWAEAMDQ
                     VRRMAAHLKSLNLEPGSHIGLISKNCAHFILADLAIWMAGHVSVALYPTLNADTIDYI
                     LNHSESKLLFVGKLDGWDEIKKGIPSGMPCISLPLAPPNDYESWDEITARTEPLRGNP
                     SRGADEPAIIVYTSGSTGQPKGVMHSFGAIAASVKGAEEIFHATAKDRMLSYLPLAHV
                     YERFCVETQGIHAGMQLFFAESLDTFVADLNRAQPTIFLSVPRLWLKFQLGVFHKLPP
                     EKLNRLLRIPLLNRVIKKKVLTGLGLQHVRIAASGSAPIPPDLIQWYRDLGLELLEGY
                     GMSEDFAYSHATYPGRGRYGYVGNPMPGVDVKISDEGEILIKSPGNMMGYYKQPELTA
                     ECYTDDGYFRTGDRGERDEDGRLKITGRVKELFKTSKGKYVAPVPIENLLNNHVHVEM
                     CCVTGSGHPQPYALILLAEELRPKLSDGKVRAEVEAELVQLLKEVNQEVEAFERLQFL
                     AVVKDPWLIENGFLTPTMKMKRSMLEDTYGPKSDDWYAAGKKVVWEDS"
     gene            24428..24697
                     /locus_tag="VFG22_09495"
     CDS             24428..24697
                     /locus_tag="VFG22_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416518.1"
                     /translation="MVTPGLSERGAYTVEAVLWDRNSASSGAGDFRPISRICTAASRH
                     DDPERADLDDFEIIADGESPPQGRAGQWLPLDLSNRTREVGCFLD"
     gene            24708..25400
                     /locus_tag="VFG22_09500"
     CDS             24708..25400
                     /locus_tag="VFG22_09500"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6416519.1"
                     /translation="MLDFSGREAVVTGGTGALGSAVVGRLVRAGATVHVPVYAPKELE
                     RFPYREHASVRLHEGLDLSVERDVERLFGGTSSLYASIHIAGGFAMGPIESTGLEVWD
                     QLMRMNATSCFLSCREAVKAMAPREGRIVNVAARPALVPTAQMSAYAASKAAVAALTL
                     SLAEELAASSIWVNAIVPSIINTAANRNAMPDADHGVWPMPDEIAETIAFLASPQNAV
                     TRGALVPVYGRS"
     gene            25473..26465
                     /locus_tag="VFG22_09505"
     CDS             25473..26465
                     /locus_tag="VFG22_09505"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6416520.1"
                     /translation="MKYTLLGRTGVMVSRVCLGCMSYGTPDWRPWVHDAEASAPFFKR
                     AIEAGINFFDTADMYSLGVSEEVTGRWLREYGNLDEIVIGTKVFFPMSDRPNMGGLSR
                     KHIQQACEDSLRRLGVDTIDLYQIHRLDPNTPMEEMLAALDQLVRQGKVRYIGASSMY
                     AWQFQRLLSMSERNGWARFVSMQNHYNLVYREEEREMMPLCEDEGIAVTPWSPLARGM
                     LAGTRSKLGDSSTSRSSSDGLDQILYDQPSDWDVVEAVKKVAAERGETPARIALAWLL
                     SKPAVTAPIVGATKLKHLDDAIAATEVVLSTEEVAILEAPYRAHPVKGLEAPKS"
     gene            complement(26478..27293)
                     /locus_tag="VFG22_09510"
     CDS             complement(26478..27293)
                     /locus_tag="VFG22_09510"
                     /inference="COORDINATES: protein motif:HMM:NF004825.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6416521.1"
                     /translation="MTRRDFKGRTVVITGGAGGIGSALARSFGRVGAKIAILDLPSSP
                     LEEAREALERAGIESVALPCDITDVEQVDRTMAAVRAALGTVDVLINNAGIVHRSAFA
                     KTRPEVFRRVMEVNLFGAIHCTQACLDDLVASRGLIIAISSIAGLAPLYGRSGYAASK
                     HAMHGLFESLGAELVEKGVGVLMVCPSFVASGLEASTLGGDGNRVSRPRSKVGQLGSP
                     DSVAADVLKAAEAGRRRLVLTPVGKTSALLSRLAPGLYERLMIRNLRSELDES"
     gene            complement(27280..28254)
                     /locus_tag="VFG22_09515"
     CDS             complement(27280..28254)
                     /locus_tag="VFG22_09515"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6416522.1"
                     /translation="MTAGPAVLVTGAAGYVGRLTVAALARRVDELSALVALDWIEVDP
                     EDRLPGVLYEQGDICDPALRELFRRHGIDTVVHLASIVRAPDGAPKDLAYRVDVLGTQ
                     NVLEACEASNAKRLIVTSSGAAYGYHPDNPEWLDEEDPLRGNDEFEYSKNKRLVENML
                     ARWREERPELRQVVFRPGTVVGEDVHSPVTDLFEKPVMLGILGADSPFVFIWDQDLVA
                     CLVDAVFSDKVGIYNQAGDGALTPREIARMLDKPYVPLPASVVKVILWTLKRLGLTEN
                     GPERVAFLRYRPVLSNRRLKEEYGYTPQLTSREAFELYARARGLLDPA"
     gene            complement(28254..30005)
                     /locus_tag="VFG22_09520"
     CDS             complement(28254..30005)
                     /locus_tag="VFG22_09520"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HET6416523.1"
                     /translation="MTQMVGGEALARMLAAEGVEVVFGIVDGTYMGFFASFQKHGIRM
                     VSPRHETSAAHMAGAYARLTGKLGVCMASNGPGVANILPGVAVESGEGNRVLLITSCR
                     RTPIAYPDRGGTYQYFNQAGVTRAMTKWSGAVPSFERLPEFFRRAARISWTGRPGVVH
                     LDVPESVMNGVFEADGIDLRPPQQYRRTEPMEPSASQVARTAELLRNAERPHLHAGSG
                     VLHARASNALERVAEALGSPVTTSWAGRGALPDNHEQLLPIWAMAPINQARSEADVVL
                     VIGSRLGETDWWGKPPYWGKAGEQKMVQVDIDEEILGLNKATELAIVSDAGTFLGALA
                     DELERSPLSEERLQANRARTAGLAEAQAKVRAQLDMALQNKSSPMHSSQVPAACQRFF
                     EDDTVYVFDGGNATVWASFFSEINRPDTLLSTFKFGMLGAGVSQALAGQIAKPDSKVV
                     CIIGDGAMGFHFQELETALRNELPVIYLVLCDKQWGMVKLTQSIGLGMLRPAIGTDQQ
                     GTINADFKEIEFDKVAQAMGCHGERVSDPAELEGALQRCVDSGKPSVIHVDVDPNMHL
                     FAPGLQEFKAMHQEPGA"
     gene            30210..31835
                     /locus_tag="VFG22_09525"
     CDS             30210..31835
                     /locus_tag="VFG22_09525"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET6416524.1"
                     /translation="MRDFLSGRFRFVILVWCIGALLLSCSVTRVKWRIKPDRELGAGR
                     SEYLEAPLAEAKPARQPNIIILLADDLGKYEVSTYGAEHISTPNIDRIGAEGVVFEEG
                     YVTAPTCAPSRAGIMTGRVQNRYGFETQIMEHYPTNWVEYISGRWIVDTGEFVVKAKP
                     SFPSEWQAHKQGIPPTEITLAEVLKKYGYTTGIVGKWHLGASRKQVPLERGFDSQFGF
                     IGAFSLYTPERNWPHVINHEHQSFSSQYQWDMGRRDEAAIVENGKVVHEEEYLTFAFR
                     DRMKAFIYEHRDEPFFLYAAFSAPHVPFQAPVDYYCRYAYVEDDNKRVYYSMISALDD
                     AIGEVHQSIKDAGIEEDTLIFLLSDNGGASYTHATDNGPLKGGKLTQFEGGINVPFMM
                     KWKGTVPAGVRYKKPVSSTDIFATSVAAAGGVLPADREYDGVDLIPYVTGEDETKPHE
                     QLFWRADHIWAIRDGKYKLILSTRDGWAELHDLEVDKSEQIDLREQMPELYEKLREEH
                     EQWQKEKLRTKPMWPRIMDKKFVLDGKEYLFPA"
     gene            31832..32473
                     /locus_tag="VFG22_09530"
     CDS             31832..32473
                     /locus_tag="VFG22_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416525.1"
                     /translation="MRKWLLISSFVLAPAVVLAAPSSGAAQQPKLDIKVFAGGSFTTY
                     VEMLETGRVRDTYPGWQAGFGPRIRKRKWFVETLLSFNRWQYSGSVAIDADLSLKLTA
                     RVNSFQLPFNAGYIPYQNAYFRLFLYGGYVNHFNTKVLASVEVPGEPTVSDKFKPKEV
                     DLAVYQALARFGLNIDLAMFNLDFNYSISMNSASTTSYRTGFHQLQLNISYLF"
     gene            complement(32489..33868)
                     /locus_tag="VFG22_09535"
     CDS             complement(32489..33868)
                     /locus_tag="VFG22_09535"
                     /inference="COORDINATES: protein motif:HMM:NF013394.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416526.1"
                     /translation="MQEAVESSLSAAAARPRRTEADAEAAREFGGTFGAAFIMSLSHA
                     LMLYLWIAWRFHDGAVFYPTGLGDVGPFFVGIWDQIVAHATPTWGTFGAYWVFLLVEG
                     LMAAFLPGLEIKGLPIPSRGGTRLVYRCNGLTAWYLSLAAVAVLHFTGVFPLETIYEQ
                     FGAFMIAAMLTANVVALFVYFGAKATGNAERMSGSFLYDFFMGAWLNPRIGPLDLKMW
                     AEVRVSWLTLFLLTASGAAHQHATYGTLSTPMIFMVVAHFLYTNACMKGEECIPTTWD
                     IFYEKWGWMLVFWNLAGVPFVYCFNSMYIASRPPFEHSIPFTVFCFVLLFGAYYVWDT
                     AQSQRNRFRMQLNGSYVKRKAFPQLPWGTLEKPRYLDTEAGSKLLVDGWWRYARKIHY
                     TADILMALSWGLICGFDHFLPYLYVTFFVIMILHRAGRDISRCKKKYGADWDRYCEEV
                     PYLFIPYVF"
     gene            33957..>34200
                     /locus_tag="VFG22_09540"
     CDS             33957..>34200
                     /locus_tag="VFG22_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416527.1"
                     /translation="MDRGSLFKIAMAGILAGVVAWIYASGAYEELDPATMRVWFRDAG
                     AWGGLLFIVTYSLFQPFGVNGLVFLLSAPLIWSPTQA"
BASE COUNT         6554 a        10685 c        10366 g         6595 t
ORIGIN      
        1 gcggcgctct gaggaccgcg actgatcatg cacaggtgtc gggcttcgga ttgcacccgt
       61 acgcctcgcg catggcccga ttccatcatc tctcgagcga tgtcgcggac gaggttctcc
      121 tgaatctgga aacgtcgagc gtatgcctcg acaagacgcg gcagctttcc gagtccgaga
      181 atgcggtctc cggggacgta ggtgatgctg acgtgcccaa aaaaaggcag aaagtgatgt
      241 gcgcaaatcg agtagaacgc gatctcccgc acctctaccc gtcctcttgc ttcgtccgcg
      301 ccgagcttgc gcgtcgtctt gagaatcgtg gacgggtcga tctcgtagcc gctgagcaga
      361 tctttgtagc atcgagcaat tcgaccctcc gcctcggggc tgtcgaacca gcgcgaagct
      421 ctcgggtcgt cggtgatgtg ttcagtgagc cagtctgtga gtttcaaggg gttctcctga
      481 gcgagcgttc gagccacgac cagatgccga gacgatccaa aagggggttc atccagcccg
      541 tgctgatgca atagcccgaa tcgtgggccg cctgatgatg gcacgcatgc gcgttcgggc
      601 gcaaagcgag gcctgcatgc tgaagaaggc gcgcccatct cggagcgggg gacatgtgcg
      661 cccatttgtg aacttgattg gcccagcatc caacagcggc catcgtccaa acgatggtga
      721 cgagagccgc cacgagcccg gactgaacgc ttggcgccac gaaggccagc gccaacaagg
      781 cgagcgccga aaagaagcaa ggctcgccat tggtctcgat ccagtcgtgc tcgaccatct
      841 gcctcggatc cacgtggtgc tctcgaaagg ctcgaatgag aagcggaccg agcaccggtg
      901 tcgaggcctc tccaaacctg tcgcaggccc agtgaactat acccgtaagc aaatcgatcg
      961 ccgcgatacc caaggggagg gcaatcagca ccgcgagcca ccacccttcg ctcatgaagc
     1021 gcccgacgct caatccgacc caagcggtca ggatcaccgc catgaagatc gatgcggcca
     1081 agaccgcgcg acgcaggaac tgcagcatgc gtatcgcaac tctacggcgg agtattctcc
     1141 cacgccagag tgagcctcgt ctgcccatcg aacctcagcg ctgccaacga ggattcttga
     1201 tgcgctcacg cttcagagcc cgcatagtgc ctcctcaaca aggaaggcgg tcgggtgtgt
     1261 tcgttcccaa aaagatgatt ccaagattgg ccgcagcggc cctcgcgctc accggttgcg
     1321 gtgacggcgg cggcacagcg ggtactggcg gcacagcggg tactggtggc acagcaggtg
     1381 ctggcggcac cgctggaacg gggggcatcg gtggcgcgct cgcggagtcg ctcgaagcgt
     1441 tctgcgtgaa gctcgtggac tgctttcccg gcgtttacgg aagcacgcaa gaatgcaccg
     1501 aatatctcac gacgtactac gggattagcg gggggaccag cgccacgtgc gaggccgcgt
     1561 tagtcagtta cctcgactgc ggcactcaac tgagttgcga agagctcacg accagcaaca
     1621 gctgcgacga cgagtttgaa gctgcctata acgcttgcac atgacgaggt gattcgcggc
     1681 gaatgctagc gacgaagcac ccgtccattt gcgagaccgg ttcgagtcga tccggcctaa
     1741 tccccgacgc cgatgtcagt cgagctgagc gcgagttgcg cctggttttc cagtgactcg
     1801 aggcgatgaa gctttgcaat ctcgctgtct agggctggac cgatctcctc gagactgaat
     1861 aaggcccagc ccttttcgat caactgttcg atagcgtaga gcgaagccgc gtagcgccga
     1921 cgtaagcgcc gacgccaacg cccaatcgac agacagccgt gatcgacctg cactccgaaa
     1981 acattctcgt ccgctagccg agaccgaagg acggacctca gaccgacgag cgcatgggcc
     2041 acctcaccct tgggtaccgg agaagtccaa gagcggagcg tcttgctcca tcccgatgtg
     2101 cacccacagg tcgcggagct ccgaaagatc ggagtccgac tctgccgccc gcatgatctg
     2161 agagctggcc gtcccgcact cgggacatac agaacttcga ggaaccgcat cgagcagtcc
     2221 accacattcg ttacattgcc aaaacatcat gtgacctcct tgagctggta cgcatgaccg
     2281 cctcgcttga atcggccgca actcgaagtc gcgaagggca ggcgattgtc ctagttaaat
     2341 tcgctaagca cgcttgccga gctgtcaacg gccgtcgcga ttttttctgg tgcaacgcgt
     2401 caagtcgctc gccgacgcca gcgagcgctc ttcgtccaac tcgcgggaac gcgcttccag
     2461 ggtcttgatc tcgtcagtcc tgtgattact gaattaagaa tcccgccgaa gaaggcggca
     2521 tcgagaaagg aggtacacat gttgacgaga tggaatgaca tcggcttcgg gggtctgaac
     2581 cgggagctcc tgtccttgaa cgaccttcgg acccggatgg accggctctt cctcgatttc
     2641 gagcgaggtt ggggaacggg tcacgatcct gaggctcgcc aagccattgc gatgggctct
     2701 gcttggcctc ggatcgtcct ctacgacgcg ggctccgagc ttcgtctgcg tgcggagctc
     2761 cccggcatgt cggaaagcga cctcgacatc accgtcgagc agagctcact caccgtgcgg
     2821 ggtgcacgcg aaactcaggt ccccgagggc tactccgtgc accgccagga gagaggaaac
     2881 cgatctttcg cgcggagctt ctcattgcct tgtcgcatcg acgccgagaa gaccagggcg
     2941 aacctgaaga acgggattct ggacatgact ctgcccaaag tccctgagga gcggccgaga
     3001 caaatcaggg tccaggtttc gtgaatcgca aaccaaagga ggtgagacga tgaatagcga
     3061 gacgacatca ttgactcaac gacaagctcc gaacccagag cggcccgagc aagccgagct
     3121 catttcgatc acgccggctg tcgacatcta cgaaaaccag gatgagatcc tggtcgttgc
     3181 cgacttccct ggcgtgccga aagactcgct ttcgatcgag ctcgatcgct ccgagcttct
     3241 catcgaagga gcgcaggcgc ccgtggaggg aggcccgcag cccatcgttt tttcgcgcgc
     3301 attccgggtg ccgaacaccg tggacccgga cggcgtgaca gcggagctga cccaaggcgt
     3361 gctcaaggtc catctggcca agtccgaggc tgccaaaccc aagcgaatcg aagtacgcgg
     3421 cggctagcgg ccgccgtccc cgccgcgtga tcgacgcgta tcccaaggcc cgggcctcgc
     3481 ccgggctttg cttatcggcc ttcgtcgctg cagaacgcca gtgagccttg gcagatggaa
     3541 ctcctcgacc caagcgcggc gcggccgccc ccgatcccca cgtcgatcga agtgatctat
     3601 cgggacgacg ccgtcgtcgt cgtcgacaag ccctccgggc tttccgtgca ccgcggtgac
     3661 gaccagggct cgaccttcgc cctcaacctc acacgcgatg cgatcggtca gtgggtgtac
     3721 cccgttcatc gtctcgatcg cgcgacgagt ggcgtgctcg tcttcgccct ctcctcggag
     3781 cacgcccggg cacttcagga atctctcgat cagcgcagct ttcacaagac ctatctcgcg
     3841 ctcgtacgcg gcacgccacc cgagcgcggc gtcatcgaca gccccatggc caaacgtgag
     3901 ggaggtcccg acgtggaatc gatcaccgag tacgaattgc tcttcgtctc cgatcgacgg
     3961 ctctcgctcg tcgaggcgcg gccgcgcacc gggcgtcgtc atcagattcg gcgccacctg
     4021 cggcgcatcg atcatccgat cgcgggtgac gtaaactacg gcaacggcgc agacaaccgc
     4081 cgctatcgcg ccgaggtcgg tctctaccgg ctagccctgc atgccaaacg gctctcgttc
     4141 ttgcatcccg ctagcggcga acgcgtcacg ttcgagagcc cggtcccgga ggatctcgcc
     4201 gagccgctgc gacgcattgg tgtgccggag acgctcttag ggacctgagg tcgaccgtac
     4261 gaagcgagct accaaatgat ctcgacgtac tctgccgcgt tcccctcatc gcttttgcag
     4321 ccgccgatca ggagcgtgag cgtcaacgca agaatcggtg ttcgtcgtga catagccatg
     4381 ggcccaagcc tccaacctct tcgaccgcgc acgtgatccg gtcgaggcta gggcgtgtac
     4441 cagattggcg acgtatacgc tcgctcttgg atcgtgagcg gctgcttgag cgtcttgggg
     4501 tcgattccgc gagcaacggc gtcatagagg gtgtgccgtg gcgtcgggat ctggagcacc
     4561 cggacgtagt agaaggcccg ttgcgtggaa tcgaaatccg ggtcggccca gaaacctctt
     4621 agcgacggtg ctcccacgga gttgagatac tgagctgtcg cggagtctac ggtatcggtg
     4681 actggcggca cctttccgtc cgggccgggc tcgcgtttgc cggaccagac gacgtcatag
     4741 attctttcgt gcgtcttccc atcggcgtcc acccagccct tgacgatttg gatgcgatcg
     4801 aggttggctc cgtcgggatc tttcacggca tacatcaaga acccgggcgc cttgtcctcg
     4861 ggggccttct tcagcttgct ccccatcggc cacccgagcg tgtaacccaa ggtgaccatc
     4921 ctggggtctc cagcctgctt cttgttgaat ccccagccgc cgaagaaccg aactcgaatc
     4981 cggggtccag tggtggcgta cacctctttg cgcttgaacg cctcgaagat cgacgcgcgg
     5041 gtgttctcct cggcccagac cgcggtgagg ccctgcgcgc tgagctcagc gcctcggatt
     5101 ccctctgttc cagactgcgg ccgatcgccg gtctccgatt ccgaggccgc tgcaccccag
     5161 aagttgtcct cctctgcaga agcgaggcct gtgtgggaat tcgtggaacc gagcaatccg
     5221 agctcgaagg gattggctcc aacggcctga ccgatggcga ggccgcggag taagccgctc
     5281 cgcgcatagt cgccctcact gacctcggcc aagggctccc atcgcattct cgaggcagcg
     5341 tacgcagccg taatcggctg cccttcactg tcgaccttcg cgaacatcgc acccttgctc
     5401 aggttcgagt tgtgaggaat cgcgagaaaa tccgtttcca cccgcgcgct ggtctcttcg
     5461 agccagctcc agagatcttc tgggcgcgcg ctgtggagcg cgctgaacgg ctcgagctga
     5521 ttcgcttgct ctgcggcgtc cttcatcaag acgatgcgat gcaaactctt cccgtcgggg
     5581 gccgaggtcc attcccagcc gatgaacgtg gtgaacgagc agggcgcgta gttgcgctcc
     5641 gcggcttcga cgatttcacc ccaggcggat gccggatcga gatcgaggtg cctaccgagg
     5701 tcctcggcgt ggtccgtcag caccatgaag tcgagcggct cttcgatctg caccttcgct
     5761 tgcgtgcgtg ggtgaacgac tggaagacct ttggcccagc ggtacgcggt atccggatct
     5821 gccgtgtggt tgcccatcgc gtaggcgtcg gacgagtaac tgctgtggac gtgggtgtca
     5881 ccccaataga gtcgattcgg ctcggatgcg atggcggcgg gagtcgtcgg cggaggcggc
     5941 ttgcaaccga tcaccgccat agcgagggcg aacagtgtcc acaaagctgg tttgatgcgt
     6001 cgcatagctg gctagtactg aatccttgcc ccgatgttcc aagtgaattg tgcgttgtcg
     6061 gtagtttgaa cgtagacgta cggcatcacg gctagcatga agaagttgag gttgtagccc
     6121 acgcccaccc gacccgtcgt ggaggtcccg aggtcgtcga agagcgctcc ggcctgcatg
     6181 aagactttga cgtgccggat cggccaccag ccgatgccac cgccgaccca ccagtcctgc
     6241 gggcctttgc cgaaaaactt gccgagcttc tggcccagca cctcgaagga gacgccattg
     6301 aactggcgat cgatgtggta ttcgtacccg atgccgatcg tcatggtctt ctcccgccgc
     6361 cccgaggtcg acttctcgcg agtgcccgag aaatccatgt gcacgtgatg catgcgctcc
     6421 cggtaatcga gcttgtagtt ctccattttt tgaggcgtcg gggaggctcc catcgcgttg
     6481 agtaacgtca gtagtccggc catcaacatg ggtccctagc cttcctctcc cttgacgtcc
     6541 ttgatgaccg gtatgtcctc gaccgacggg cgatcgacga ttgccggaga gccatgcacc
     6601 tccgcattgc agcggtcaca ttgggctgga ttgaaccgaa tgacggttcc atgaacccat
     6661 cgaccataga tgttgatggc cggaatcacc ccatcgcctc ccaaagcgca gaccttgggc
     6721 cccacgacct tgcggaagcg ctcttcgtcg ttcggcagct gctgtgcccc gaacgcatcg
     6781 atgtcgatga cgccgatctc ctcgtaaggc tcgactggct cggcgatcgg atttcggatg
     6841 acgcgataat cgcagtcggg gccccgaccg gcgtaagtgc cgcccgtctt gcgaacctct
     6901 ccctggcccc cgcaggcgag catgcaagtc gcggggagca gcagcaatga aacaaacaag
     6961 atagacgcaa cgttcatttt gtccgcggac gctagcacca cggatcgagc tcgtacaatc
     7021 gtcacgaggt ccccgaggca acttggtatt ctccatcact tcgggtaaga aaggcgttca
     7081 agcatcctct ccccaggtcg acaatggaac ccttgcctcg attcgtcccg ctggatcgga
     7141 cgatcgcctt cagcgacggc gtctttgccg tcgtcatcac cgtcttggtg ctcgggatcg
     7201 agattccctc cgatgctgtt ttgacgggag cggcgcttgc ggtggagcgc atgaagctcc
     7261 tccatcaact cctcatctat gtcgtcgctt tctggatggt cgggatgtat tggtcgcagc
     7321 acagcctctt tttcgccggc ctcaagcaga tcgaccgggg gatcgtggtc ctcaatctct
     7381 tgtttctatt gcttgtgacc ctgctcccct tcgtcacgca gctcatgggc gcgaagcggc
     7441 acgactggaa ggtcgttttg gtcttcggtg tggtcaatct attcgcgacg ctcgtgctcg
     7501 aacggatgtg gagccgtatc gcggccatgc ccgaagcgca caagagccct cagacggcga
     7561 tgctcgccgc gaggattcgg atcggagtgc gcgtatacgc aggcgtgatg gtcttgggag
     7621 tcctcgtcgc gtcgctcgat gttcgagccg ggactttcgt gttcattctg atcccgatcg
     7681 cacacttcta caactacctc cgcgatccgt ttcgctctcg ctttggcggg gtatcctcgg
     7741 gcgcaaacaa gtgagctgag tgccgattct cgccatcgat ctcgggacat cgaacgtgaa
     7801 ggccgccgtg gtcgaccgca aaggcacact gctcggcacg ggtcgtggtt cgatcgaaac
     7861 gctgcacacg gccgacggcg gagccgagca ggatcccgag cttgtatggg aggccgtgct
     7921 gaacgcaacc gatcaggccc tcgcatctct ggcaaaccgg cgtgatgttc gcggcgtagc
     7981 ctgcgcgagt cagtactcct cgatcgtccc ggtcgacgcg gccggcagcc cgacggggaa
     8041 catggtcgtc tggatggaca agcggggcgc gcccgagcgg ctacggcagc tgccgggcgg
     8101 gacgcaaatg aagccaagtc tcctgcagat ggcccgctgg cttcagatcc acggcattcc
     8161 gcctctcgct tccggaagcg attccctagc gcacatgcgc tggatcaagc tcgcgcgccc
     8221 tgaggtctac ggacggactg cgacgttttt ggagccgatg gattacgtga cggcgcggct
     8281 ctccgggcgg cgcgtcacaa acctgtgctc gagcttcatg atgctgctga ccgacaaccg
     8341 gcgccccaat cccgcgtacc atccaaccct gttgaaatgg tctggactgg acatcgaaaa
     8401 actgccggag ctcgtcccca tcgacgagcc acttgggccg attctacccg ctatcgccga
     8461 gcgcctcggg ctcgccccag acacgctggt cttcccaccg ctcaacgaca cacaggccgg
     8521 cggaatcggc gcgagcgcct tcgctggaga acacgccggg gtttcgatcg gcacgaccgg
     8581 cgtctgcatc acccacgtcg acttcaagaa gaccgacgtc ctcaattctc tggcaagcat
     8641 gccgagcccg ttcccgggcc gcaacttcgt catggccgag gcgggcatcg gtggtggggc
     8701 agtcaagtac ttcctcgagc atctcgtctt cgcacgcgac cgcttcggcg accacagcct
     8761 cgaagagagg ttttcggcac tggaacgcgc catcgagccc atcgagcctg ggagcgacgg
     8821 ggtgctcttt ctcccctggt tgacgggctc gatcacgccg gcggaagacg ggaaggtgcg
     8881 tgggggtttc ttgaatatct cgctgcgaac gacgcgagag catttggcaa aagccgtgct
     8941 cgaaggggtt gcgttcaatc agcgctgggt tcaggagcgc gtggagcgct ttgctaagcg
     9001 tgacatcagc cgaatcaagt tctacggagg gggtgcgctg tcatcggcat ggtcccagat
     9061 cttcgccgat gtcctgaatt gcccggtgga acagctccga gacccggagt ttgccgtgag
     9121 ccgcggcgtc gctcttcttg gtttccatcg gctcggcgag ctcgccctcg acgagatcga
     9181 agccggcgtt cccgtggccg ctgtctacca gccccggccc gaggcgagcg tccgctacga
     9241 cgcgatgttc actcagttcg tccgcgtctt caaaaagaac cgcgatgtat ttcgcgcgtt
     9301 gaacgttggg agctagtcat gagcgacacg atcgaagatc tgatgcgagg cctctatccc
     9361 tatgcggaga gccacggggt gatccgcgct ttccccgagc aaggccgctc gaccgatgcg
     9421 attctcgacg agcttcgtgc gattgccagc caagaagatc aagcttggga gaacggccgc
     9481 tgctcgggaa cgatgtacag cggtgaccat gagcactacg atttcttgaa cgcggtcttc
     9541 tccctattct cgtacgtcaa ctcgctgcag cgagacatct gtccgagcat gacccgtttc
     9601 gaatccgaca tcatcgcgat gaccctcgac ctcatgcatg gcgaggaggt ccgcaagcaa
     9661 aacccggctc agaaggctgt gggcgcggtc ggattcggtg gaacggagag catcatcaac
     9721 ccattgctga tctatcggga caaggcccgc gccgagcgag gcatcgatcg cccgaaggtc
     9781 atcgttccgg acactgccca ccccgcattc cgaaaagggg cccaccttct cggaatcgaa
     9841 gtcgtggatg cgcctacaca ccccaagacg accgaggtcg atctcgactt cgtgcgcgac
     9901 aggctcgacg cacggaccgt ggcgatcgtc ggttccgcag gcaactaccc atacggaacg
     9961 atcgatccca tcgaggcctt gtcggagctg gctctcgagc acggggtcgg actccacgtc
    10021 gacgggtgcc tcggcgggtt catccttccc tggggccagc ggcttggtta cgagataccg
    10081 gtcttcgatt ttcggcttcc tggggtcacg tcgatctccg cggacaccca caagttcggg
    10141 tacggactca agggcacttc cgtggtggtc tatcgagacg cgtccttccg caagtaccag
    10201 tatcacatcg agcctatgtg gaaggggggg atctatgtgt ccaatgggct tgcgggcagt
    10261 cgctccggcg gcctgatagc agcgacctgg gccgcgatgg tgcgctacgg aaaggagggc
    10321 tacctgcgct acgccaagca gatcttcgag acctcgttcc ggatgcaaga ttcagtcaag
    10381 agccatcccg acctgagcat catgggaacg ccgactttct gcttttcgtt tcgttcggag
    10441 gaattcaaca tctaccacgt caacgatttc atgaagacgc gcggttggcg tttcaatggg
    10501 cagcagcacc cggcggccat tcacatgtgc gtgacgcggc ctcaaaccag gtccgggatc
    10561 gcggaggcat tcgaaacgga ccttgctgag gcggtcgcct atgcgcgcaa acaccgtgac
    10621 gccgaaccga agagctcagc gatctatggc ggcggggcta ccgggttgcc gaccgattcg
    10681 gccgagggga tcgagctctt catggctatg gcactcgata ccctccagtc ctatccgttc
    10741 tgagaccctc gcgggctaaa aggtcgcaac cccctcgaac gccggagtag aatgacggat
    10801 tggggaagag catgacgagg atctacatac tcgcgttcac cggcatcgcg gtgattgcag
    10861 cgctcgacta ctggaccggg cacgagatct ccttcgccgt attctatttg ctgcccgtgg
    10921 cgctcttggc atggcggacc agtttgcgga ccgcaatcgc cgctgcggtg ctctctgccg
    10981 tcctttggta ttcagcagac gtgtcgagcc atgaatatag ccacccggcc atcggcgtct
    11041 ggaacgccat cgcgcgttct atcatcttca tcttcgcggc ggttgcgctc gaccgcgttc
    11101 ggactctgct cgagcgtgag agggcgcttg cccgaaccga cccccagacg ggactctaca
    11161 atctgagagc attcgaggag ctactcgagc atgagctctc gaggacccag cgcacgggtc
    11221 gcgcgttcac gctggcgtac atggacctcg accgcttcaa ggccgtgaac gatcgtttcg
    11281 gtcatgcggc cggtgatttg gtcttgcgtg aggtaggcac gaagcttgcg acgactcttc
    11341 ggggcatcga cgcgacggca cggctcggcg gcgatgagtt tgcgatcctt ttgagcgaga
    11401 ctgacgaagc tcaagccgtg ctcgcattag cgcgcattca gagggccatc gcgctgacgc
    11461 tccggagtat tcgcgcggct tctgaaatcg gagtcggtgc aagcattggt gcggtcgtct
    11521 attccagcgg aacggccacc tcgggcgacc tgctcggaga agccgatgcg ctcatgtatc
    11581 gggccaagca ggagagaccg ggctccatac ggatcgaatc ccccgaagct tggcgcacca
    11641 gcacgagtcc tgcagcggtg atgactcgtt cggaaccgtg acttcggcac cacttggtga
    11701 tagctgccgg ccatgaccca gactgccggt tctgccgagg accaagcgcc tccctgggag
    11761 acgctccaaa gactcatcga cgctcaagac cgccccgccg cccaagcctt tgtgcaagga
    11821 gtgcccgctg gggacctacc gcgtctcttg agcttgctcg gcaacgaaga gagcgcgcgc
    11881 ctcctcgaga tgctcgaacc cacgacgagc ggcttcctga tggagagcat ccccgaggcc
    11941 caggccgccg aggtgctggg agagctcgat tcagacaagg ccgcagaaat cgtcgcgcac
    12001 ttgccgagta atgagcaggc cgatctaatc gcgagccttc ccgatgacga agcccaggcc
    12061 atcttggagc agatgccgcc ggaggacgcg gccgacgccg agcgtctagc gcagtacccc
    12121 gaggggaccg ccggcgcgct gatgatcacg gagtacctga gctttcctca tgacgtgacg
    12181 gttggagcgg tccttcaaga tctccgtcaa aacaccgagc gctatcagcg ccacgacgtg
    12241 caatacatct atgtcacgga tgcagccggc atcttgcgag gcgtgctgag gttgcgagac
    12301 ctcgtgctca cgccagaacg gaccccggtc gagcagatca tgatcacggc catggaggcc
    12361 gtaccggttc aagcgcctct tgccactctt cgcgacttct tcgctgcgcg ctcctaccga
    12421 ggcgttcccg tcactgaccg cgctggccgc ctcgtcggtg tcgtgcgcca tgccgacgtc
    12481 gcagaagccg tgggcgagcg cgaggtgagc gaccacctca aggcgcaggg catcgtcggc
    12541 ggtgaagagc ttcgcaccat gccgaccctc acccgcgcct ggcgccgctt gtcctggctc
    12601 agcatcaata tcctgctcaa catcatggcc gcgagcgtca tcgcatttta tcaagagacc
    12661 ctcgcggcgg tgatcgtgtt ggccgtgttc ctgccgatca tctccgacat gagcggctgc
    12721 tcgggcaatc aggcgatcgc cgtcagcatg cgtgagatca cgctcgggct cattcgtccc
    12781 tccgatgcac tacgcgtctg gagcaaggag cttgccgtcg gtctcatcaa ctctgtcgcc
    12841 ctggggactc tcctcggcct ggccgcgtac ttctggcaag gcaacatcta cctgagtctc
    12901 gtggtcggcc tcgccttggc ggtcaatacg atcgtctccg tttcgctagg gggcctggta
    12961 ccgctcttct tacgcggcat gcgattcgat ccggcgctcg catcaggtcc gatcctgacg
    13021 accgtcaccg acatgtgcgg gttcttcctg gtgctcagct tcgcaagtgc catgcttccg
    13081 ctcatcggag gctagggcgc gattcgctgc ccgtctcgcc aatcgagaac ttggaagaaa
    13141 cagacaatgc cctcagtcat tcctacgcgc atcgctccaa gcgtggcgcc cgccgacctc
    13201 agccctgcga aggtgtcccg gagctttcac caactcgtag aatccggtgc gaagatccgc
    13261 gctgccggtg aagcgaaaag cgacccaatg ctgctgctgt cttccggcta caccccgaaa
    13321 tatcggatcg atctctttga cacccatttc tacttcagca acgtgcttca gaatccggcg
    13381 ttgcgattct tcgtcgctta cgtcgtccag aaacgcccga cgaccggtcg aaccgagatc
    13441 tttccgcgta ttttctacaa ggacctgtcc ctggtctggc gctgcgcctc tcacgtgata
    13501 gcgaccgatg gcgacttctg gatcggcaag ggagacgtca aggtcctggc ccggggtggc
    13561 ttcgaggtca ccgagtgcgt ggagtcgacc acggacctgc ccttcgaaat gcaagcggcg
    13621 ctcgagacgc tcaaccgcaa gacgcggcac gctcgcatgg acgaggaggc gatctatctc
    13681 gtgctccgat ccgcgccaag ctcccgaact gcaccgtacc gggactttac cgagccccgg
    13741 cgcaaagccg ctgccgaccg ccgtaacctc atcaacggcg gccgcagcat cgcgcggttt
    13801 actcgcaaga acgatccgac ttcgctccaa atcgtcgatg ggttcgtgcc cgatttcacc
    13861 aagggcgtcg tcgaagtgag ccagataaag agcgccatgt acggcgggat gctcgagcgg
    13921 tttcgcattc tttctcgcaa ccggaagatt cagtacctct ttaccgctgg gcccaagcac
    13981 gtgtggatca ttccgcccca ggctctgacc accgaacttt cgagctacgg cgtgcgcacc
    14041 gtcgatgtcg tcgccgacga ggatctattc gttccgggct acgagtatca ctacttcgac
    14101 gaatcggcag acgagtcgga gcacttcagc cagatccccg aaggcttcgc cggcgagctc
    14161 agcgagcacg acaacgaccg agccgacgca tcggcctggc tcgatcagat cccggtcatc
    14221 cgcgagttcc ggcgcaaggt aatgggccgg aagcacaagc cgtttcggtt ttgagaagcg
    14281 ctacgaggct gctgccagtt gcggttgccg tttccgttag cccgggaggg gaggcgtcct
    14341 accatgaccc gcggcaatct gcctctattg ctcgatgcag tatagccgga ggccgagcga
    14401 gcacttcggt gtgtcgttgg ccgtccagtt ggcgcccgcc gacgcggtcg tcccacagag
    14461 cccgatgcca tcggtgccgt tggtgaagcc ggagcaatcg ccctgggtat agggcagtcc
    14521 gttcggccgc gtgcccgtcc agacgtcccc accacggagt tgtccgctgg cgtccaagtt
    14581 gatcggcacg aggatcgaac cgtcgaccag atcgtcccag tcgttggcga caggggttcc
    14641 gtcgaccagc acgtacggct cgctcgattg cgtgagccgg tccgagaccg aggagaccac
    14701 cgtcgagagc caggccttga actctccgtc gagcccggcc gctgaggcct gagacgcaca
    14761 gagcgcatcc gcgccatcga gcccgccgaa gtcggcgttc tgaacgctgg ccgtgacgaa
    14821 gattcggcgt tcgagaacgc tcgcgtcgag gccggagtcg atgcccgcat caccgggcga
    14881 ctcggtcgat gcacaggcca gtagcccgag cgaggcaagg agaagtgtgg gcttcatttc
    14941 ctcgctttcg catcccagct ggggcgacgc aacagcagtc ttggtgacac gtgagcgctg
    15001 ctcctacacc ggccggctcg atccccgatg tcgatttgct gtttgctcga ggacggattg
    15061 tgctaatcac ggccgcaaca agcatgtgtg gggtctcccg atggctgtag atagttcgat
    15121 tgtcgaatgg tccttgccaa gtggctatgc cgcctcttcc gtcgaggcca gggataccgg
    15181 cttccgagga atgggcctct tcgctcggcg gctcatccga gagggggaga cgatcgtggc
    15241 atttggcggc accgtcatcg accgcgccgg cctcctcggt ctgccgcggt ccgcgattcg
    15301 gcataccttg cagatcgacg aagagctctt tctggtttcg gacaggccgg ggccggccga
    15361 ttacgtgaat cactcctgcc agcccaatgc ggggctctgc ggccaggtgg tgctggtcgc
    15421 catgcggacc atttctcgag acgaagagat cacctacgac tacgccatgt ccgacgcctg
    15481 cgcctacgac gagttcccgt gctcctgcgg gtcgattcag tgccgcggcg cggtctcggg
    15541 ctccgactgg gcccggcccg atctctggaa gcgctacccg gggttctttt ctccttatat
    15601 tcaaagccaa attgaagggc ttcgccggct gtcgaggcgc acgggacgca aggtacgaag
    15661 catccgatga gtccccccaa cgaggacggc ggagctcctg cggctaagca gacgggcaag
    15721 gcttccggag tcaaggttcg ccgcgccacc ctcgaagaca ttccgggcat ctacgcgtgc
    15781 gagatcgcgg cgtaccctca aattccaccc cacaccttgc ccgccgcccg ggagctccag
    15841 ttcgagatcg aagcctttcc agacggacag ctcgtcgccg tttccggaga taccgtcgtc
    15901 ggctacgcca gctcgctcat cgttcagctg gacgacgact cgccttggta ctcctggggc
    15961 gagatcacgg gtaatggcac cttcagcacg cacaccccga gcggcgacac cctctacgga
    16021 gccgacatcg ccgtccatcc cgacttccga ggacgcggca tctcgaagaa gctctaccaa
    16081 gggcgcaaac ggatcctccg gcgtttcaat ctacgcagga tggtcgcggg tggtcgcatt
    16141 cccggctacc aggagcacgc gggtagcatc acagccgagg agtacgtcgc ccgcgtgatc
    16201 cgtggcgagc tccgcgatcc ttcactcaac gcgcaccttc atgcgggcta ccacgtcaat
    16261 tccgttcata tggggtacgc gcgcgacgag cagagtctca actatgcgac tttcctcgag
    16321 tacctgaatc cgaatttcag cccggaaagg cgcagcatcg cgggggcccc gctcaagcgg
    16381 cccgtccgtc gcatccgggt ctgcgcggcc cagtacgaaa tgaggaggat ctcctcctgg
    16441 gaagagttcg agcaacaggt cgatttcttc gtcgcgaccg cggagcaata caacagccat
    16501 ttcctctgct ttcccgagct cttcaccgcc cagctcttca gcatgatgcc ccccacgatg
    16561 cagccgctcg aggcggtgcg tgagctggca tcgatggccg accgctacaa agagatgttc
    16621 gtgaagcgcg ccaagaagag cggcatcttc atcatcggag ggtctcatcc ggtggtcgtc
    16681 gacggcaaga tgttgaacgt tgcccatctc ttcacgccgt ccgggaacgt ctacacgcag
    16741 tccaagcttc acatcacacc aaacgagcgg gccgagtacg ggatccaacc cggagatggg
    16801 cttcacatct tcgacacggg ccatgcgagg atcgcgatcc tggtctgcta cgacgtcgag
    16861 tttcccgagc tcgcgcgaat cctcaccctg gctggcgccg aggtgctctt cgttccgttc
    16921 agcaccgacg agcgcaaagc ctatctccga gttcgctaca gcagccaggc ccgtgccgtc
    16981 gagaacatgg tctacgtggt tctctcgggg aacgttggca acctcccgca agtcgacaac
    17041 ttcctgatca actacggcga ggcgatcatc tgcacgccga gcgactttgc gtttccgcct
    17101 gatgccattg cggcgaaggc cgacttcaac acggagaccg tcgtgatcag cgacctggat
    17161 ctgggtgcgc tcgagcaagt ccgagagctc ggaagcgtcc gaccgcttcg tgaccgccgg
    17221 ccggacctct accacatcgc gccgagggaa ccggtcaaga tcattagaac tcgctagcgg
    17281 cgatcaatcc gacgcgatct cctgaagtcg cacgttatac caaggtcccg ttccgattcg
    17341 atcccgtgca ttctctgtcg aggtgctgag gtatgcgcgc cagtttcggt cgccagctcc
    17401 caccgcatcg gccagggtcg agcgccgaga cccctctggc aagcggccgc aggccagggg
    17461 atactcctct acgatgagcg aatcggccga caacggactc tctgcttccg agcaactgct
    17521 cgagatcgaa gcgatcaagc agctcaaggc gcgctacttt cgcttgatgg acagcaagga
    17581 atggctgccc ttttctcaag ttttcaccga cgacgtagtc gtcgacgtgt cgcaggacaa
    17641 gcatgcgccc ggcgacacga tcgaagggcg ggtcaacgtg gtcgacttca ttcgcaaggc
    17701 ggtcggtaaa gccacgaccg tgcaccacgg gcacatgccg gaaatcgaga tcttgagccc
    17761 gactgaagcg cgcggcgttt gggccatgtt cgactacgtc gagttcaggc cctcgcgtgg
    17821 catccgcgga tacggccact atcacgagga gtaccgcaaa gagcgcgggc gctggcacat
    17881 ctgcaagctg aagctgacgc gcctcagagt ggacccgctc ggctgagccc ggcggttctc
    17941 ctgcacccga cgttccaaac cagtccgtgg ccgacttgtg aacgttgcgg agatcgagca
    18001 caagcacgct cgtgcccatc ttggttgcag cgggtctgag caaggcatac ggggagacgc
    18061 gcctgctcga tggagtggac ctatcgattc acgaagggga gcgcatcggg ctcgtgggca
    18121 acaacggctc gggcaagtcg acgctcgctc gcatcttggt cggtgaggag ccccccgatg
    18181 aggggcgagt cgcgcgccgt cgtgaagcga gcatccgtta cctcccccag gaaccgacct
    18241 tgcccgccgg gcgcagcgcg cgcgaggtag tcctcgaagg tctgggcgcc tgggcggagg
    18301 cccatgcgcg gcacgagaag ctcagtcggt tgatcgcggc gcaagagcag ggctgggagg
    18361 cctattcgga gcctcaggcg gcagcggccc aggaagtgga gcgactcgga ggctgggaga
    18421 acagtctgcg agctgacgtg ctgattcagc agcttgggct ccatgacatc gaacgcagcg
    18481 tcgacgaaat gagcgggggc gagcggcggc gcgttgcgct cgcacgactg ctggtcgaac
    18541 agcccgcgct ggcgattctc gatgagccaa ccaaccatct cgacgcggac acgatcgaat
    18601 ggctcgaaac ctaccttcgc gagcagtatc gcggagcgct cgtcttgatc actcacgacc
    18661 gctacgtgct cgatcgagtc gtgactcgaa cgctcgaagt ggatcaggga caggtataca
    18721 gctacgaggg cggttggcac gcttacctcg aggcgaaggc agagcgcttg gcgcatgcga
    18781 accgtgcgga agccaatcgc caaaatcttc tcagaacgga gctcgaatgg cttcgaaggt
    18841 cgcccaaggc gcgcaccacg aagtcgaagt cccgggtgga tagggtcgag gcgctccgcg
    18901 atcagaccgc gcccaaaggg gagcaagtgg cgcagttcgc ggcgcagacg cagaggctcg
    18961 ggaacacggt gctcgaggca cagagcctcg gagtttcgat cggtacactg gagctcgtcg
    19021 aatcgctgga tttgatcctg tcgcgcgggg atcgcctcgg catcgtcggt ccgaacggag
    19081 ccggaaaaac gacgctgcta cgcgttctgc tcggcgagct cgagccagcg agcggccggg
    19141 tcgtcgttgg cgccaatacg aagttcgcat acttcgatca gggccgaagt ggtctcagcg
    19201 aagacgattc cgtccagcag aacgtcgcgc ccgaccggcc tcaggtgacc ttccaaggtc
    19261 gctcgatgga cattcggagc tacttgaagc gcttcctctt cgctccccat cgtacgcgtg
    19321 acaaagtctc ctcgctttct ggcggggaac gggctcgcgt cgccctcgcc aagctgctgc
    19381 tggtcccagc caacgtcctg gtgctcgacg agccgaccaa cgacctcgac gtgtccacac
    19441 tctccgccct cgaagagatg ctcgtcgaat ccaactcgac ggcgctcatc gtcagccacg
    19501 atcgctattt tctcgaccgg gtcgcgaaca agatcctcgc cttcgaaggc ggctgtcgtg
    19561 tggtcgcgta tgcaggcaac tacagcgact atcgcgaaca gcgaaatgcc gctcagatag
    19621 atggcaagag gggaaccgaa aaaggcccca aggctccgaa gacatcgtct gcggtcaaag
    19681 tgcagaagct ctcgtataag gagaagcaag agctcgagag catcgtgggc gagatcgagc
    19741 gtgcggaaag ggaagcagcc gaagccgagg ccctcttgaa cgatccgatg ctctatgcgg
    19801 accgtgccgc acaggtgcca gcgatagtgg cgcgccaagc agagcttcga gcccaagtcg
    19861 accgattgat gagccgctgg atcgagctcg aagccaagag caaccctggt taggctcgca
    19921 gctggagcat ttggaagcgc tgtacgatgt ccggccgctt catgagaagc tcgctgaatc
    19981 gcggcactgg catacgcaga attaggacct cgctcgcggc ctcgatggtg actcgagacg
    20041 cttcgcgggt cagcagcgac tcttggccga tggggtagcc caggtccatg tttcctaggc
    20101 gtcttccgtc cgcggtacgc actacgagtc gtccgtgtag tggaagatac atggcgtccg
    20161 agcgtttacc ggcttcgacg attctagtcc cagcgtgtgc ccggcgaact tcgaacaggc
    20221 gcgccagctg ctttcgagtg acctcgtcaa acgcggtgaa gatcggattg gttcgcagca
    20281 gcgttgcgat gagccggcga ctcaggactt cgagaagcac gtcaccgaac ggaggatgcc
    20341 ttgcgacgag ccgatccaga agatcctttc cgattcgaag ggcgcgaacc ctgccgcatg
    20401 cgacgacttc ctcgccgtaa ctcacgttgg agagcaggca cgaaacccca gcgacgtcgc
    20461 cctcgccgag caacatgctc tgcatgtccg agccacgcct tcccaccgca ttcccttcga
    20521 ccagaacgta cagcgcgtcg gcgctcgttc ccgcagtcgt gaggcgctgt ccgtcctcca
    20581 cgtcgacgag cccggattcc ctcaccatgg tctgaagtaa accgcgcgga acttccgcga
    20641 agagcggcat cgatggaagc tgtgcgagcg ttgccgcgct tggacgctgg cttacgagag
    20701 cgtcgctcgg cttcggcctt tcgagcagct cgacgttcgt gagatcgagg gtgatgtctt
    20761 ccgtcttctc gggtttcatg aatcgggttt ctccgtccgg ggctcggcgg tcggcgatca
    20821 acggaagcgc gtcggtgatg aggctctgag tgtctgtcgg cgtgccgtca tcgtcctcaa
    20881 cgtctgaatc gtccgcggtg acgatcagcg cccggttttg acgatggagt cttcgtgcgg
    20941 cctgcggatc gacccgctcg agcacctcgc ccttgctcgg gtcgatcgcc aagatcagct
    21001 tggccattgc cgccgcccgg gccacgaaac ccttggcagc gtagaggtcc acggcccgct
    21061 cgtaggcgag caccgcgtcg gccttgcgtc ccattcgctg caggagatcg ccgcgacggt
    21121 gcgaccatcg aggctcatcc ggtgtttgct tctcgatcag ctcgtagaga tcgagcgctt
    21181 cattgagccg ctcggccttg agcgcgttgc cgagctgcgt tcgcagattc gtcgtcttgc
    21241 ttgggcgtct gaacatccgt cccccctctc ggaacgaaac acacaattca ccctagcggg
    21301 ttagaggtca gacccccaaa tccaagttcc aagcccctca aatcacagca aattgaagcc
    21361 gcgcaggtcc aggctgaggc ggcaacgacg cccgttgact ggcagaagcg agtatcgcaa
    21421 ggctcccgag gaggagagca acatgaccga gattgtccgc tacgaacgct ctggccccgt
    21481 cggaaccatc acgatggacg atggaaaagt gaactgcatg tcgcttgcca tgctctcgga
    21541 gctgaatcgc gcgctcgacc aagcgcaggc ggacggcgtg gctctcgtga tcacgggccg
    21601 tgaggggatg ttctcaggcg ggttcgatct cgccaccttc aagcaggggg gggaggcgct
    21661 ctatcagatg ctcaagggcg gtgcagagtt ggtcgagcgc gtcatgtcgt ttccgaggcc
    21721 catcgtcacg gccgcaggag gtcacacgat cgcgatgggc gcgttcttgg cgatcggcgc
    21781 agacgcccgg attggagcgg agggggcgtt caagatcgta tgtaacgagg tcgcgattgg
    21841 gctgactctg ccgagatttg cgatcgagat cattcgacac cggctcaccc ccgcgcactt
    21901 caaccgtgcg ctcgtactcg ctgcgcctta ttcgcccaag gaggccgtcg aggcgggttt
    21961 cttcgatcgc gtcgtccctc cgtcagaagt tctcacggaa gccacgaacc tcgcgcaggc
    22021 actcagccag ctcaacgcga aggctcatgc tgaatccaag ctcctcgtcc gagaaagagc
    22081 gctcaacgct ttgcgagagg ccatcgagtc cgagctcggc tcgttccaaa tcttcgccga
    22141 taccatcggc agcgggatga aagtgagtcg ctagcgcgag agaggggctt gctcagccat
    22201 ccccgttccg cacttcgatg atctcgcaag ttacctctcc cttggggcgc cgaaccgtga
    22261 tctcgtcacc gacttcgcgt gacaagagcg atcgccccat cggagagtcc atgctgatcc
    22321 acttcttctt ggcgtcgatt tcgtctgggc caacgattcg gtaggtctca gattggccat
    22381 tcgggtgttc gatcgtgacc cagctcccga aatacacgcg cccatcgtcg ggcggctttt
    22441 cgtcgacgat ggtcaagacc ttgagccgcc gccccaagaa tcgcagccgt cgatcgatcg
    22501 cggcgagtcg ctgcttgctg tactgatact cggcgttctc ggaacgatcc ccttccgccg
    22561 cggccacttg cactgccttt gccatcttgg gccggtcgac gttccacagg cgatcggcct
    22621 cctcttcgaa acgacggtat ccctccggcg tgatgtagcc agggtcgccc gaagtatccg
    22681 gtcgtgttcg ttgccgtgac atggtgctct acgcgtcgtt cagcccatcg atgccccggc
    22741 gcccgtgact cggttcgttc aggagtcttc ccagacgacc ttcttcccgg cagcgtacca
    22801 atcgtcggac tttggcccgt aggtatcctc gagcatgctt cgcttcattt tcatcgttgg
    22861 cgtcaggaat ccgttttcga tcagccacgg gtccttcacg accgccaaga actgtaggcg
    22921 ctcgaatgcc tcgacttcct ggttgacctc tttcaagagc tgcaccagct ctgcttcgac
    22981 ctccgcgcgc accttgccat cgctcaactt cggacggagc tcttcggcga gaaggatgag
    23041 cgcgtacggc tgtggatgcc cggaaccggt cacacagcac atctccacgt gcacgtgatt
    23101 gttgagcagg ttctcgatgg ggaccggcgc gacatacttt cccttgctcg tcttgaagag
    23161 ctctttgacg cgaccggtga tcttgaggcg gccgtcttca tcgcgttccc cgcggtcgcc
    23221 agtcctgaag tagccgtcgt cggtgtagca ctccgcagtc agctccggct gcttgtagta
    23281 gcccatcatg ttgcccggag acttgatcaa gatctcccct tcgtcgctga tcttcacatc
    23341 gacgccgggc atcgggttgc cgacgtaccc gtagcgaccg cgacctgggt atgtggcgtg
    23401 cgagtacgcg aagtcctcgc tcattccata tccttcgagg agctcgaggc ccaagtcccg
    23461 gtaccactga atgagatcgg gtggaattgg agccgaacca ctggccgcaa tccgcacgtg
    23521 ctggagcccg agcccggtca gaaccttttt cttgatgact cggttgagca gcgggatacg
    23581 cagcagccgg ttcagcttct ctggcggaag cttgtgaaac acgccgagct gaaactttag
    23641 ccagaggcgg ggtaccgaaa ggaagatcgt cggttgggca cgattgagat cggccacgaa
    23701 ggtatcgagc gactcggcga agaagagctg cattcccgcg tgaatgccct gtgtctcgac
    23761 acagaaacgc tcgtacacgt gagcgagcgg cagataggac agcattcggt ctttggcggt
    23821 tgcgtgaaag atctcctcgg cgcctttcac cgatgcggcg atcgcgccaa agctgtgcat
    23881 cacgcccttc ggctgcccgg tgctgcccga tgtgtacacg atgattgcag gctcatcggc
    23941 gccgcgcgac ggattgcctc gcaacggctc ggttcgggcg gtgatctcgt cccaggattc
    24001 gtagtcattg gggggggcta gcggaagtga aatgcaaggc atgccgctcg ggatgccctt
    24061 cttgatttcg tcccatccgt cgagcttgcc gacgaaaaga agcttcgatt cactgtggtt
    24121 caggatgtag tcgatcgtgt cggcgttcag cgtcggatag agcgccacgc tcacgtgtcc
    24181 ggccatccaa atcgccaggt cagcaagaat gaagtgcgcg cagttcttcg agatgagccc
    24241 gatgtgactg ccgggctcca ggttgagcga cttgaggtgc gcggccatcc gcctcacctg
    24301 atccatggcc tcggcccagg tgtacgtttg gacctggcca ttgccaatcg gctgcgtcat
    24361 ccaaagttgg tttgggaccg tcttttccca gcggtatgcg tcatcgagta cgagttgagg
    24421 ctcggccatg gtcactcctg gtttgtcgga gcggggagca tacaccgtcg aagcggtgct
    24481 gtgggaccga aattcggcat cttcaggagc cggtgacttt cgtccgatct cgcgaatctg
    24541 cactgctgct tctcggcacg acgaccccga gcgtgcagat ctcgatgact tcgagatcat
    24601 cgccgatggt gagtcgcccc cgcaaggccg cgccggccag tggctcccgt tagacctcag
    24661 taacaggacg cgtgaggttg gctgcttcct agactaagct ctcgcgcatg ctggatttct
    24721 caggtcggga agcggtcgtt accggcggca cgggtgcgct tggcagtgcg gtggtcgggc
    24781 gtctcgtccg ggcaggggcc acggttcacg ttccggtcta cgccccgaaa gagctcgaga
    24841 gatttccgta tcgagagcat gcctcggtga ggcttcacga agggctcgac ttgagcgtcg
    24901 aacgtgacgt cgagcgtctc tttgggggga cgtcttcgtt gtatgcgagc attcacatcg
    24961 caggtggctt tgccatgggc ccgatcgagt cgaccggact cgaagtctgg gaccaactca
    25021 tgcgaatgaa cgccaccagt tgtttcctct cttgtcggga ggccgtgaaa gcgatggctc
    25081 cacgcgaggg ccgaatcgtg aacgtcgccg ctcgcccggc gctcgtgccg accgcgcaga
    25141 tgagcgcata cgccgcttcg aaggccgcgg tggctgcgct gactctctcg ctcgccgaag
    25201 agctggccgc gtcttcgatc tgggtgaacg ccatcgtccc gagtatcatc aacaccgctg
    25261 cgaatcggaa cgcaatgcct gacgcggacc acggagtctg gcccatgcca gacgagatcg
    25321 cggagaccat cgcgttcctc gcgtcgccac agaacgccgt tacccgcggc gccctcgtgc
    25381 ctgtctacgg gcgaagctag atttccaggc ggcgggacga aggcaaacgg aatgatgatc
    25441 gtttaccgat cgatttcggt tagtattcgg ccatgaagta cacgcttctt gggcggaccg
    25501 gggttatggt ttcgcgggtt tgcctcggtt gcatgagtta cggaacaccg gactggcgac
    25561 cctgggttca cgacgcggag gcttcggcgc ctttcttcaa gcgagccatc gaagcaggga
    25621 tcaatttctt cgataccgcc gacatgtact cgctcggggt gagcgaggag gtcaccggac
    25681 gttggcttcg cgagtacgga aatctcgatg agatcgtcat cgggacgaag gtcttcttcc
    25741 caatgagcga ccgtcccaac atgggcggtc tctcgcgcaa gcacattcaa caggcttgcg
    25801 aggacagcct gcggcgcctt ggcgtcgata cgatcgacct ataccagatc cacaggctgg
    25861 acccgaacac gccgatggaa gaaatgctgg cggcgctcga ccaactggtc aggcaaggaa
    25921 aggttcgcta catcggcgcg agctccatgt atgcgtggca gttccagcgc ctgctctcga
    25981 tgtccgagcg caatggctgg gcgcgcttcg tctcgatgca gaaccactac aacctggtct
    26041 atcgcgaaga agaacgggag atgatgcccc tctgcgagga cgagggcatc gccgtcactc
    26101 cctggtctcc actcgcgcgg ggtatgctcg ccgggacccg aagcaagctt ggcgacagct
    26161 cgacgagtcg ctcttcgagc gacggcctcg atcagattct ctatgatcaa ccatccgact
    26221 gggatgtcgt cgaagccgtg aagaaggttg ccgcggagcg cggtgagaca ccggcccgta
    26281 tcgcgctcgc gtggctgctt tccaagcctg ccgtaacggc gccgatcgtg ggagccacga
    26341 agctcaagca cctcgatgat gccatcgccg cgacggaggt cgtgctcagc acggaagagg
    26401 tcgcaattct cgaggcgccg taccgcgcac atcccgtcaa gggcttggaa gcccccaaga
    26461 gctgagcaca gcgatcgtca gctctcgtcg agctccgagc gcaggtttcg gatcatcaag
    26521 cgttcgtaga gacctggagc gagtcgcgaa aggagtgcgc tcgtcttgcc gaccggagtg
    26581 agaacgagcc gtctgcgacc cgcttccgcg gctttcagca cgtcggcggc gaccgagtcc
    26641 ggactgccga gctggccgac cttcgaacgt ggacggctca cgcgattgcc gtcccctccg
    26701 agggtcgagg cttccaggcc ggatgcgaca aacgacgggc acaccatcag caccccgaca
    26761 cctttttcga ctagctctgc accgaggctc tcgaatagcc cgtgcatggc gtgcttcgat
    26821 gcggcgtatc cacttcgacc gtagagtggc gccagtccag cgatgctcga aatcgcgatg
    26881 atgaggccgc ggctcgcaac caggtcgtcg agacacgcct gcgtacaatg aatggcaccg
    26941 aagagattga cctccatcac acggcgaaaa acctcgggtc gagttttcgc gaatgcgctt
    27001 cgatgaacga tgccggcatt gttgatcaat acgtccaccg ttccgagagc agcgcgaacg
    27061 gccgccatcg tccggtccac ctgctccacg tctgtgatgt cgcaaggcag ggcgaccgac
    27121 tcgataccag cacgttcgag cgcttcacgg gcctcttcca aggggctcga cgggagatcg
    27181 aggattgcga tcttggcccc gactcgcccg aagctccgcg cgagcgcgct tccgatgcca
    27241 ccggcgcctc cggtgatgac gaccgtgcgc cccttgaagt cacgccgggt caagaaggcc
    27301 ccgggcccgc gcgtagagct cgaaagcttc gcggctcgtg agttgggggg tgtagccgta
    27361 ctcctccttc agccgccggt tcgagagaac cggccggtag cgcaggaacg caacgcgttc
    27421 ggggccattc tcggtgaggc ccaggcgctt cagtgtccag aggattacct tcacgaccga
    27481 cgcgggcagc ggaacgtagg gtttgtccag cattcgagcg atttcgcgag gtgtgagcgc
    27541 cccatcacca gcttggttgt agatgccgac tttgtccgag aagacggcgt cgacgagaca
    27601 ggcgaccaag tcctggtccc agatgaagac gaaaggcgag tccgccccca ggatgccgag
    27661 catcaccggt ttctcgaaca gatcggtcac ggggctgtgc acgtcctcgc ccacgaccgt
    27721 ccccggacgg aacacgactt gccgaagctc cggtcgttct tcgcgccatc gcgcgagcat
    27781 gttttcgact aggcgcttgt tcttcgaata ctcgaattcg tcgttgcctc gaagtgggtc
    27841 ctcctcgtcg agccactccg gattgtcggg gtgatatcca tatgccgcgc cactcgacgt
    27901 gacgatcagg cgcttggcat tcgaggcttc acaggcttcg aggacgttct gcgttccgag
    27961 cacgtccacc ctgtacgcaa ggtccttcgg cgcaccgtcc ggcgcacgga caatcgaagc
    28021 caaatgcacg accgtatcga taccgtgccg gcggaacagt tccctcagcg cggggtcgca
    28081 gatgtcacct tgttcgtaga gcacgcccgg aagacgatcc tcgggatcga cctcgatcca
    28141 gtccaaggcg acgagtgccg acagctcgtc tacacgtctc gctagtgccg cgacggtcag
    28201 gcgaccgaca tagccggccg ccccggttac caagacggca gggcccgccg tcattaggcg
    28261 ccgggctcct ggtgcatcgc cttgaactct tgaagcccag gcgcgaatag gtgcatgttc
    28321 ggatcgacgt cgacgtgaat gaccgagggt ttgccggagt cgacgcagcg ttgaagagcg
    28381 ccttcgagct cggccgggtc tgagacccgt tctccatgac agcccatcgc ctgagcgact
    28441 ttgtcgaact cgatctcctt gaagtccgcg ttgatcgtgc cttgctggtc ggtgccgatg
    28501 gcgggccgca gcatgccgag ccctatcgac tgcgtgagct tcaccatgcc ccactgcttg
    28561 tcgcagagaa ccaagtagat gaccggaagc tcgttgcgaa gggctgtttc gagctcttga
    28621 aagtggaaac ccatcgcgcc gtcgccgatg atgcagacga ccttggagtc tggcttcgcg
    28681 atctgccccg cgagcgcttg cgagacgcca gcgccgagca tcccaaactt gaacgtcgag
    28741 agcagggtgt ccggacggtt gatctccgag aagaagctcg cccagaccgt agcgttgccg
    28801 ccgtcgaaca cgtagacggt gtcgtcttcg aaaaagcgct gacacgccgc cggaacctgg
    28861 gagctgtgca tcgggctcga cttgttttga agcgccatgt cgagctgggc tcgaaccttc
    28921 gcctgggctt cagcgagtcc ggccgttcgc gcgcgattcg cttgcaagcg ctcctcgctg
    28981 aggggcgagc gttcgagctc gtcggcaagc gcgccgagaa acgtgcccgc atcggaaacg
    29041 atcgcgagct cggtggcctt gttgagcccg aggatctctt catcgatgtc gacttggacc
    29101 atcttctgct cgccggcctt tccccagtaa ggcggcttcc cccaccagtc ggtctcgccg
    29161 agccgtgagc cgatcaccag caccacgtcg gcttcactgc gggcctggtt gatcggcgcc
    29221 attgcccaga taggcagcag ttgctcgtgg ttgtcgggga gggcgccgcg ccctgcccag
    29281 ctcgtcgtga ccggcgagcc cagggcttcc gcaacccgtt cgagcgcgtt ggacgcacgc
    29341 gcgtgaagca caccgctccc tgcgtgcagg tggggacgct cggcgttgcg aagcagctcg
    29401 gcagtgcgcg cgacctggct cgcggagggc tccatgggct cggtccgccg gtactgttgc
    29461 gggggccgaa ggtcgatgcc atcggcttcg aaaaccccat tcatgaccga ttcgggaaca
    29521 tcgagatgca ccacgcccgg ccgcccagtc cagctgatgc gcgctgcccg tcgaaagaac
    29581 tcggggaggc gctcgaagga aggaactgcg ccgctccact tcgtcatcgc acgcgtcacg
    29641 ccagcctgat tgaagtactg ataggtgcca ccgcgatccg gataggcgat cggcgtgcgc
    29701 ctgcacgaag tgatcaacag aacgcgattg ccctcaccgc tctcgaccgc gactccggga
    29761 aggatgttcg ctacaccagg cccattcgat gccatgcaaa cgccgagctt tccggtcaat
    29821 cgcgcgtaag ccccggccat gtgcgcggca ctggtttcgt ggcgaggtga gaccatccgg
    29881 atgccatgct tctggaagct cgcgaagaag cccatgtagg tgccgtcgac gattccaaac
    29941 acgacttcga ccccctcggc agccaacatg cgagcgagcg cctctcctcc aaccatctgg
    30001 gtcatggcga tccttcctgc gtctttgtgg ccgccaaccg cgagcggctc gggacgccga
    30061 ggcaagaatc tacacccacc cgtttcggag ctgaaagcga aacttttgcg cctggaaatc
    30121 catccgagat tcgccaaagg ctgcgcgtca cgacctcgat cctgctagtt ttttgcccgt
    30181 gccatacgtt cgctagaaac tcttcgtcga tgcgagactt ccttagcggt cgtttcaggt
    30241 tcgtgatcct cgtttggtgc attggagcgc tcctcctgag ttgctcggtg actcgcgtga
    30301 agtggcgaat caagcccgac cgcgagctgg gtgcggggcg ctcggagtac ctagaagcgc
    30361 cactggccga agccaagcca gcgcgtcagc ccaacatcat catcctcctg gccgacgact
    30421 tggggaagta cgaggtgtcc acgtacggcg cagaacacat ctcgactccc aacatcgatc
    30481 gaataggggc cgagggcgtg gtcttcgaag agggctatgt gaccgctccc acctgtgcgc
    30541 cctcacgcgc tggaatcatg accggtcgag ttcagaatcg ctacggcttc gaaactcaga
    30601 tcatggagca ctatccgacc aactgggtgg agtacatctc cggtcgatgg atcgtcgata
    30661 cgggcgagtt cgtcgtcaag gccaaaccgt cgtttccctc cgagtggcag gcgcacaagc
    30721 agggcattcc tccgacggag atcacgctgg cagaagtcct gaagaaatac ggctacacga
    30781 ctggcatcgt cgggaagtgg cacctcggtg cttctcgcaa gcaggtgcct ctcgaacgcg
    30841 gtttcgactc tcagttcgga ttcatcggcg cgttctcgct ctacacaccg gagcgcaact
    30901 ggccgcatgt gatcaatcac gaacatcagt cgttcagctc gcaataccag tgggacatgg
    30961 gacgccggga cgaggctgcg atcgtcgaga acgggaaggt cgtccatgag gaggagtatc
    31021 tcaccttcgc gtttcgcgac cgaatgaaag cgttcatcta cgagcaccgc gatgagccgt
    31081 tctttctcta tgcggcattc agtgcgcctc acgtgccttt tcaggcgccc gtcgattact
    31141 actgcaggta tgcctacgtc gaggatgaca acaagcgcgt ctactactcc atgatttccg
    31201 cgctcgatga tgcgataggc gaagtccatc agagcatcaa ggatgctgga atcgaagagg
    31261 acaccctgat ctttcttctc agtgacaacg gcggggcctc ttatacgcat gcaaccgaca
    31321 acggcccgct caagggcggt aagctcaccc agttcgaagg cggtatcaac gtccccttca
    31381 tgatgaagtg gaaaggcacg gttccagcag gggttcgcta caagaagccc gtctcttcca
    31441 ccgacatctt tgcgacttcc gtggcggctg ccggtggtgt gctgcccgcc gatcgagagt
    31501 acgacggcgt cgacttgatc ccgtacgtca cgggcgagga cgagaccaag ccgcacgagc
    31561 aactattctg gagagcagat cacatctggg cgattcgtga cggcaagtac aagctcattt
    31621 tgagcacacg tgatggctgg gccgagctcc acgacctcga ggtcgacaag tccgagcaga
    31681 tcgatctgcg cgaacagatg ccggagctct acgagaagct ccgtgaggag catgagcagt
    31741 ggcaaaagga gaagctgcgc acgaaaccga tgtggccgcg catcatggac aagaagttcg
    31801 tgctcgacgg aaaggaatac ctctttccgg catgaggaag tggctactga tttcgtcgtt
    31861 cgttctcgcg ccggctgtgg tcctggcagc gccttctagt ggtgctgcac agcagcccaa
    31921 gctcgacatc aaggtcttcg ccggagggag cttcacgacc tacgtggaga tgctcgagac
    31981 gggccgagtg cgagatacat atccgggctg gcaggcgggt ttcggtcctc ggattcgcaa
    32041 gcgcaagtgg ttcgtggaga cgcttctttc tttcaatcga tggcagtact cgggatccgt
    32101 ggctattgac gcggacctca gcctcaagct caccgcacgg gtgaactcgt tccagcttcc
    32161 cttcaatgca ggatatatcc cctaccagaa tgcctatttc aggctgttcc tctacggcgg
    32221 ttacgtgaac cacttcaaca cgaaggtcct cgcgtccgtc gaagtccccg gcgaaccaac
    32281 cgtctcggat aagttcaaac ccaaggaggt cgatcttgcc gtttaccagg ccctcgctcg
    32341 attcgggctc aacatcgacc tcgcgatgtt caacctagac ttcaactact cgatttcgat
    32401 gaacagtgcg agcaccacct cttaccgcac tggtttccat cagctccagc tcaacatttc
    32461 ctatttgttc tagccataga acccaggctc agaacacgta gggaatgaag aggtatggca
    32521 cctcttcgca gtagcgatcc cagtccgcgc cgtacttctt cttgcaccga ctgatgtcac
    32581 gacccgcgcg atgcaggatc atgatcacga agaaggtgac gtagagatac ggaaggaagt
    32641 gatcaaaccc gcagatcaag ccccaggaga gcgccatcaa gatatcggcc gtgtagtgga
    32701 tcttgcgcgc gtagcgccac caaccatcga ctaacaactt ggatccggcc tccgtatcga
    32761 gataccgcgg cttctcgagc gtaccccagg gaagctgcgg gaacgctttg cgtttcacat
    32821 acgagccatt gagttgcatt cgaaagcgat tgcgctggct ctgcgcagtg tcccagacgt
    32881 agtacgctcc aaagagaagg acgaagcaga agacggtgaa tggaatcgag tgctcgaaag
    32941 gcggtctcga cgcgatgtac atcgagttga agcagtaaac gaagggcact cctgccaggt
    33001 tccagaatac cagcatccag ccccacttct cgtagaagat atcccatgtc gtagggatgc
    33061 attcctcacc cttcatgcag gcattcgtat agagaaagtg tgcgacgacc atgaagatca
    33121 tcggagtgct cagggttccg taagtggcgt gttggtgtgc ggcgccgctg gcggtcagaa
    33181 ggaagagggt cagccaggag acgcgaacct ctgcccacat cttgagatcc aagggaccga
    33241 tgcgcggatt cagccaggcg cccatgaaga aatcatagag gaagctgccg ctcatccgtt
    33301 ctgcgttccc agtcgccttg gctccgaaat agacgaacaa ggccacgacg ttggccgtga
    33361 gcatggcggc gatcatgaac gcgccaaact gctcgtagat cgtctcgaga ggaaacactc
    33421 cagtgaaatg cagtactgcg acggcagcca ggctgagata ccaggccgtg agtccgttgc
    33481 agcgatatac gagacgcgtt ccaccgcgcg acggaatcgg caaccccttg atttcgagcc
    33541 ctgggagaaa agccgccatc aagccttcga cgagcaagaa cacccagtaa gcaccaaacg
    33601 tcccccaggt cggcgtcgca tgagcgacga tctgatccca gatcccgacg aagaatggac
    33661 cgacgtcgcc gagacccgtc ggataaaaga ccgcgccatc gtggaagcgc caagcgatcc
    33721 acaagtacag catgagcgcg tgcgagagcg acatgatgaa agccgcgcca aaagtgcctc
    33781 caaactcacg cgcagcttcg gcatcggcct cggtccttcg aggacgcgcg gctgcggctg
    33841 acaaactgga ctccactgct tcctgcatgc cgacctcctg actgtccggg ccattttttt
    33901 cacactccaa ctttgaagtc cagcgtctct gaggcgacct gcgctaggct ctgcggatgg
    33961 atcggggaag tctcttcaag atcgcaatgg ctgggatcct tgccggtgtg gttgcgtgga
    34021 tctacgccag cggggcgtac gaggagctcg accctgcgac gatgcgagtc tggttccggg
    34081 atgcgggtgc ctggggcgga ctgctcttca tcgtcacgta ctccttgttc caacctttcg
    34141 gggtgaacgg cctcgtgttt ctgctcagcg cgccgttgat ctggagcccc actcaagcct
//
LOCUS       DASRZF010000212         2422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_144094, whole genome shotgun sequence.
ACCESSION   DASRZF010000212 DASRZF010000000
VERSION     DASRZF010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144094"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VFG22_09545"
     CDS             <1..656
                     /locus_tag="VFG22_09545"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HET6416528.1"
                     /translation="ERMAGSQIAMLRKMVDSGEMDMVTKVRAIRVNTGLGKAVPAAAK
                     SRPTPSTTPSSAGDPLVQSIQRDLVALGYDPGNTRGELSTPTVVAISKFQAENNLEVT
                     GEATPQLAGILAAKRDAAASSTATASSGTESLEEAQAACLQQKIDAVKKKKQGFGRFM
                     KAAANTASRYGGTKVSNEVEKASAEAYKADATAKDLEEAADALGLSKADIESCRNPT"
     gene            684..1307
                     /locus_tag="VFG22_09550"
     CDS             684..1307
                     /locus_tag="VFG22_09550"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6416529.1"
                     /translation="MDLKALGTEVRDRLLRAAALEIARHGFKGASLRGVAARADIRAA
                     SIFHYFPGGKEELARAMLEHIMETIGTRMTPTIDAGSSLPPADLILECAAQLWDFMAE
                     YPEYAGALMREAFEPDEETIVEVVRENGQRIVKLASAYIKTAQTSGQLGSFNVERFLF
                     RLASFTITFHASPSMRRQILGARFSAATEREAFLTSIREEITGNDKT"
     gene            1352..2200
                     /locus_tag="VFG22_09555"
     CDS             1352..2200
                     /locus_tag="VFG22_09555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6416530.1"
                     /translation="MMSIEGKNVMITGPTEGIGRATAFALAERGARLHLLCRNPTKAA
                     ALRDALLSRYGDSRVELYPADLGNFAEVRRAASAFIARNEPLDVLINNAGVINTRRIL
                     LDNGQEQMFAVNHLGHFLLTTLLLDRLMQAEQGRVVVVASEAYRFCRGIRFDDLQWNE
                     GFSAFPTYGHSKLANILFARELAERVRDTRVTVNAVHPGAVRSQLGAQNTWYAGRILV
                     LLKPFLRTAERGAETSIYLATSDEVTTTSGEYFYDCKPKKTKRTACDQEQARRLWELS
                     ERLVAA"
BASE COUNT          509 a          769 c          746 g          398 t
ORIGIN      
        1 tagagcggat ggcgggctcg cagatcgcga tgttacgcaa gatggtcgac agcggcgaga
       61 tggacatggt gacgaaggtt cgggcgattc gagtcaatac ggggcttggc aaggcggtcc
      121 cggctgccgc gaaatcccgg ccgaccccat ccactacgcc gtcttcggcc ggcgatccgc
      181 tggtgcagtc gatccaacgc gatctcgtgg ccctcggcta tgaccctggc aatacgcgtg
      241 gcgagctgag cacgccgacc gtcgttgcca tctcgaagtt tcaggcggag aacaacctcg
      301 aggtcacggg cgaggcgacc cctcagctcg ccggcattct ggccgcgaag cgcgatgctg
      361 ccgcttcctc cacggcgacc gcgagctccg gcacggagtc attggaggaa gcccaggcgg
      421 cgtgcctcca gcagaagatc gatgcggtca aaaagaagaa gcaggggttt ggcagattca
      481 tgaaagccgc cgccaacacg gcatcccgct acggcggcac caaggtcagc aacgaggtgg
      541 agaaagcctc cgccgaggcc tacaaagcag acgccacggc aaaggacctc gaagaagccg
      601 cagacgcgct cggcctctcg aaagccgaca tcgaatcttg ccgcaatccg acatagcgaa
      661 cggctgaggc gcagcacggg tgcgtggatc tgaaggcgtt gggtaccgag gtccgcgatc
      721 gcttgctccg ggccgccgcc ctggaaatcg cgcggcatgg cttcaaaggg gcgtcgctgc
      781 ggggggtcgc cgcacgcgcc gacatccgcg cagccagtat cttccactac ttccccggtg
      841 ggaaggaaga gctcgctcgc gccatgttgg agcacatcat ggagacgatc ggcacgcgca
      901 tgacgccgac gatcgacgcc gggtcgagcc ttccaccggc cgacctcatc ctggaatgcg
      961 cagcgcagct ctgggacttc atggccgagt accccgagta cgcaggcgcc ctgatgcggg
     1021 aagcattcga gcccgatgaa gaaacgatcg tcgaagtcgt gcgcgaaaac gggcagcgca
     1081 tcgtaaaatt ggcctccgct tacatcaaga ccgcgcagac cagtggccag ctcggaagct
     1141 tcaacgtgga acgtttcctg tttcgcctgg cgtcattcac catcacattc cacgcctctc
     1201 ccagcatgcg acgccagatc ctcggcgctc gcttttccgc tgcaacagaa cgagaggcgt
     1261 tcctgacgtc gatacgcgag gaaatcaccg gcaacgacaa gacctgaggc ggcgtgtgta
     1321 gacgcccgaa tgcagtacag agaacgctat gatgatgtcg atcgagggca agaacgtgat
     1381 gatcaccggt ccgaccgagg ggatcggacg agccacggcc ttcgccctgg cggaacgagg
     1441 cgcacggctt cacctgctct gccgcaatcc aacgaaagca gcagcgcttc gggatgcgct
     1501 cctctcgagg tatggcgact cgcgcgtcga gctctacccc gccgatcttg ggaatttcgc
     1561 cgaggtgcgc agagccgcga gtgcgttcat cgcccggaac gagcccctcg acgtgcttat
     1621 caacaacgcc ggagtcatca atacgcgccg gatcctgctc gacaacggcc aggagcagat
     1681 gttcgcggtc aaccaccttg ggcatttctt gctcacgacg ctgctgctcg atcggctgat
     1741 gcaagccgag cagggccggg tcgtcgtcgt ggcgtcggaa gcctatcggt tctgccgcgg
     1801 tattcgtttc gacgatctcc aatggaacga gggcttcagc gcctttccga cctacggaca
     1861 ttcgaagctc gcgaacatcc tgttcgctcg ggagcttgcc gaacgtgtgc gggatacccg
     1921 cgtgaccgta aacgcggttc accccggcgc ggtccgtagc cagctggggg cgcagaacac
     1981 gtggtatgcc ggtcgcatcc tggtgctgct caaacccttc ttgcggaccg ccgagcgagg
     2041 cgccgagacg agtatctacc tcgcgacgtc cgacgaagtg acgacgacca gcggcgagta
     2101 cttctatgac tgcaagccaa agaagaccaa gcgaaccgct tgcgaccaag aacaggctcg
     2161 acgcctctgg gagctgagcg agcgcctagt cgcggcctga cggacttgcg cagcctcgtc
     2221 gaccctcgcc accgtgcccg ccgttaacga aaaacgcgga catcgcgcga ccgatcgacc
     2281 gctgaaaact gaaccggaat cgtgcagagc tcaaacaacg tgctctgcat agccactact
     2341 cggatatgac cagatacatt tctcttgcga gaaaatgctc cttagaaagg aggtgatcca
     2401 gccccaggtt cccctagggc ta
//
LOCUS       DASRZF010000213         4484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2552520, whole genome shotgun sequence.
ACCESSION   DASRZF010000213 DASRZF010000000
VERSION     DASRZF010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2552520"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1334)
                     /locus_tag="VFG22_09560"
     CDS             complement(<1..1334)
                     /locus_tag="VFG22_09560"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HET6416531.1"
                     /translation="MHTAQSFPSRSTFCALLLLITTASCSSASPEVDVEAGPVALRRL
                     TADQYSSSIRDVLGKHITVPRRIDPDDRRDGLLAVGASFASVTPSGFEKYEAAATAVA
                     DQALDASHRDELVQCQPASIASSDDECARAFIERVGRRLFRRSLTTAEAEARLSIASE
                     AANALGDFYEGLELSLTSLLVSPEFIFRVEIAEAIPRDPSRNRVTSIAMASRVSYFLW
                     NTTPDEELLSAGENGDLVDENGLAQQVDRMLESPKLEAGIRSMFSDLYDFKQFDDGLV
                     RKDTALFPVYTQTLVEEAKEQTLRTIVAHVSEEEDYRDLFTTSESFMTRRLGVVYQVP
                     VATANGWEPYSFSEDTLRAGLLSHVSLNALHSHPGRSSATLRGKFVREVLLCQDIPSP
                     PANIDFSIVENTTGELRTARERLEKHVTNDSCAGCHSLMDPVGLALENFDAVG"
     gene            1514..1894
                     /locus_tag="VFG22_09565"
     CDS             1514..1894
                     /locus_tag="VFG22_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416532.1"
                     /translation="MLRKFVLITGFLDIPIGIATWAAAILEPQDAHFGALMTCGAFLV
                     FAGAALLWSAQEMKTRAPIIFWQGFVRLTAVASILYMVPRGIAESWQYGIVAFDGAIA
                     LVYIFGMIQHSGASFFQLLSGKSG"
     gene            1949..2962
                     /locus_tag="VFG22_09570"
     CDS             1949..2962
                     /locus_tag="VFG22_09570"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET6416533.1"
                     /translation="MVRLAAFGVFLVVLHLGQPIVIPILLATLIAISLSTIIDLSERG
                     LPHWVAVVLACLAAVTVVSGLGFLTTRAATDFALAFGQYRGDFDRLQFETASWLWSLG
                     LGIPATAVEQFDPGELVRGLTVPGLTLALSVVSSGTLVLLITVFLVVESGSISTKLAG
                     TDRIGRIDVQVLKGAARDVQRYLLIKTVTSAVTGLLVAALTGVVGLGHSLLFGLIAFV
                     LNYIPSIGSILASIPAIALGLVTLGWLPAFGLAFGYFLINLVIGIMIEPRWAGQATDL
                     SPSVVVLSMVFWGFVLGPIGALLSVPLTIIVRITASQSAEWSWLAMLLSSPLSVARTA
                     DSV"
     gene            3129..3305
                     /locus_tag="VFG22_09575"
     CDS             3129..3305
                     /locus_tag="VFG22_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416534.1"
                     /translation="MKQQIRTDAQGRPIVTGISKGTDPGTFEQYAAVQLGEDIHYGVG
                     AAILALIETSGLPE"
     gene            complement(3348..3821)
                     /locus_tag="VFG22_09580"
     CDS             complement(3348..3821)
                     /locus_tag="VFG22_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416535.1"
                     /translation="MLGGRPEIGEQPEGVGIEFDVAMWPLVFVTMPPTIRMNDIVYLQ
                     QSYEHVFAAPMRHALVVDTTTIENIPDATLRRHMKDFEDGRRAIIREKNIGSAIVLSN
                     SLVRGAYTALRWISPQPAPNKAFSNVRDAARWCVEGIQADGQEVPAAAYILAGML"
     gene            complement(3830..4255)
                     /locus_tag="VFG22_09585"
     CDS             complement(3830..4255)
                     /locus_tag="VFG22_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416536.1"
                     /translation="MWPLVLISMPPITTTSDIGYMQACYEHVFAAPTRHALVVDTTSI
                     VRVPDATLRREMKAFEDSRRPIIRDKNIGSAVVIQNSIVRGAYTALRWISPQPAPNKA
                     FPTLLDAARWCVEEIEADGQVVPIAAYALAGLVSSAAAR"
BASE COUNT          777 a         1404 c         1365 g          938 t
ORIGIN      
        1 cccaccgcgt cgaagttctc gagggcgaga cctacagggt ccatcaagct gtgacagcct
       61 gcgcaagagt cgttggtgac gtgcttctcc aagcgctcac gcgccgtccg aagctccccc
      121 gtggtgttct ccacgatcga gaagtcgatg ttcgcaggcg ggctcggaat gtcttggcaa
      181 agcagcacct cgcggacgaa ctttccacgc aacgtggcag agcttcggcc cggatgggag
      241 tgcagcgcgt tcaggctgac gtgtgagagc aagccggctc tcagtgtgtc ttcggaaaag
      301 gaatagggct cccagccgtt cgccgtagct accgggactt ggtacacgac accgagcctt
      361 cgagtcatga aggactccga tgtggtgaac agatcccggt agtcctcctc ctcgctcacg
      421 tgcgctacga tcgtacggag cgtctgctcc ttggcctctt cgaccagggt ctgggtgtag
      481 acgggaaaca gcgccgtgtc cttgcggacc aggccgtcgt cgaattgctt gaagtcgtag
      541 aggtccgaga acatcgaccg tatgccggcc tccagcttcg gggactcgag cattcggtcg
      601 acctgctgag cgagaccgtt ctcatcgact aggtctccgt tttcgcccgc cgaaagcaac
      661 tcttcatcag gcgtcgtgtt ccacaagaag tagctgacgc gggaagccat cgcgatgctg
      721 gtcacgcgat tcctggaggg atcgcgcggg atggcctccg cgatctcgac acggaagatg
      781 aactccggcg aaaccaatag gctcgtcagg gagagctcta gcccttcgta gaagtcaccc
      841 agcgcgttgg ccgcctcact tgcgatgctc aggcgcgctt cggcctctgc cgtagtcaag
      901 gatcgccgaa agagtcgcct gcccacgcgc tcgatgaaag cccttgcaca ttcgtcgtca
      961 ctagaagcta ttgaagctgg ctggcattga acgagctcgt cccgatgtga tgcgtcgagc
     1021 gcctggtcgg caacggcggt cgccgcggct tcgtactttt cgaaacccga tggcgtcacg
     1081 ctggcaaagg aagcacccac ggccagaagt ccatcgcgtc gatcatcggg gtcgatccgg
     1141 cgcggtacgg tgatgtgttt cccgagaaca tcgcggatgc tactcgaata ctgatctgcg
     1201 gtgagacgac ggagcgcgac cggtcccgct tcgacgtcga cttctggcga cgcgctgctg
     1261 cagcttgccg tcgtgatcaa gaggagaagc gcacaaaagg tcgagcgact tggaaatgat
     1321 tgggcggtgt gcacctgggg tcctcttgat ggatgcgcag aagccccgga cggtacgccg
     1381 ttgtcacgat tctcgcaatc gcctgtctgg atcttggagc catacggcac cgttcgcgcg
     1441 ctgaggtgac actcgaggat gggtcgcata gactccgccc cgcacgctcg tacgagctcg
     1501 gaaggagacc tgagtgctta gaaaattcgt tttgatcacc gggtttctcg atatcccgat
     1561 cgggatcgcg acctgggccg ccgccatact cgaaccccag gacgcacatt tcggcgcgct
     1621 gatgacctgc ggggcctttc tcgtcttcgc cggagctgcg ctcctctggt ccgcgcagga
     1681 gatgaagacg cgcgccccga tcatcttctg gcagggcttc gtccgcctca ccgccgttgc
     1741 gagcattctt tacatggttc caagaggcat cgccgagtcc tggcagtacg gcatcgtcgc
     1801 cttcgacggc gccatcgccc tcgtctacat cttcgggatg atccagcaca gcggcgcgtc
     1861 attcttccag ctactgagcg gcaagagcgg gtaggggcgc cttcgacggc gccagcgcgc
     1921 gcgtctacgt ggtgctagcc tgcgtcccat ggttcgcctg gcagcgtttg gggtgttctt
     1981 ggtcgtcctg cacttgggac aaccgatcgt aattcccata ctgttggcta ccctgatcgc
     2041 gattagcctc tcgacgatca tcgacctcag tgagaggggt ctgccccact gggttgcggt
     2101 cgtattggct tgtctggccg ccgtgaccgt cgtctccggg ctaggttttc tgaccacgcg
     2161 ggctgcgacc gacttcgcgc tcgcgttcgg ccagtaccgg ggtgacttcg atcgcctgca
     2221 gttcgagacg gcgtcctggc tctggagtct cgggctgggc attccggcca cagccgtgga
     2281 gcagttcgac ccgggcgagc tggtgcgcgg gctcaccgtt cctggtctga cgctcgcgct
     2341 cagcgtcgtc tcttcgggca cgttggtcct cctgatcacc gtgtttctcg tggtcgagag
     2401 cggctcgatt tcgactaagc tcgccggaac ggaccgaatc ggaaggatcg acgtgcaggt
     2461 cctcaaaggc gcggcgcggg acgtccaacg ttatctgttg atcaagacgg tcacgagtgc
     2521 ggtcaccggg ctactcgttg cggcacttac cggcgtcgtc ggtctcgggc actctctcct
     2581 gttcgggttg atcgccttcg ttctcaacta catcccgagc atcggaagca tcctcgcttc
     2641 gattccggcg atcgccctcg gcctcgtcac gctgggttgg ctgccggcgt tcggattggc
     2701 tttcggctac ttcctcatca acttggtcat cggcatcatg atcgagcccc gctgggccgg
     2761 gcaggccacc gacttgtcgc catcggtcgt cgttctttcc atggtcttct ggggtttcgt
     2821 gctcgggccg atcggcgcgc tcttgtcggt cccgttgacg atcatcgttc ggatcaccgc
     2881 ttcgcagtcg gctgaatggt cctggctggc catgcttctg agctcgcctc tttcggtcgc
     2941 aaggactgcc gactccgtct gatcggcggc ctctggcacc ccttcccgag aatgccactc
     3001 cgagcccgcg ttttccgccg ggctaagcta gtagacgcat ctcagtgatg aggctcccga
     3061 tatgcgaacg acaaagccgg cggctctccg cactcgcgca ttgctctgcg cggcgcgcag
     3121 tcgcaggcat gaagcagcag attcgaaccg acgcgcaggg acgacccatc gtcacgggta
     3181 tttcgaaggg caccgatccg ggcaccttcg agcagtacgc ggctgtgcag ctcggagaag
     3241 acattcacta cggggtcggg gcggccatcc tcgcgctcat tgaaacctct ggccttccgg
     3301 aatgacccgg ggactgcttg gttgcgcgcg cagagcgcgc cagcgcgcta caacatgccg
     3361 gccagaatat aggcagccgc cgggacttct tgaccgtccg cttggatgcc ctcgacgcac
     3421 cagcgcgcgg cgtcgcgcac gttgctgaag gccttgttgg gcgcaggctg cggtgagatc
     3481 caccgaagcg ccgtgtacgc accacgcacg agcgagttcg acaagacgat cgcactaccg
     3541 atgttctttt cgcgaataat cgcgcgtctt ccgtcctcga agtccttcat gtggcgccgg
     3601 agggttgcgt ccgggatgtt ctctatcgtc gtcgtgtcca cgacgagcgc gtgtcgcatc
     3661 ggagcggcaa agacatgctc gtaggactgc tgcagataga cgatgtcatt catgcgaatt
     3721 gtcggcggca tcgtgacgaa aaccaacggc cacatggcca cgtcgaattc gatcccaaca
     3781 ccttccggct gctcgccgat ctctggacgt ccccccaaca tgatctacct catcgggcag
     3841 cagcgctgct cacgagaccc gcgagcgcat aagcggcgat cggtacgacc tgcccgtccg
     3901 cttcaatttc ctcgacgcac caccgagccg cgtccagcaa ggtcgggaag gccttgttgg
     3961 gagccggttg cggagagatc cagcgcaacg cggtatatgc cccgcgcacg atcgagttct
     4021 gaatgacgac cgcgcttccg atgtttttat cgcgaattat cggacgtcga ctgtcctcga
     4081 acgctttcat ctctcgtcgc agcgtcgcat caggcactct cacgattgaa gtcgtatcga
     4141 cgaccagtgc gtggcgagtc ggtgccgcga aaacgtgttc atagcaggcc tgcatgtaac
     4201 cgatgtcgga tgtcgtcgtg atcggcggca ttgaaatgag aacgagtggc cacattgaaa
     4261 catcgaaccc aagccccacg gcgggtggtc gtcccgcaat cctcggcgcg ttccccatgg
     4321 cgcgattatc ggcattgcgg ctgccgaagc aaactaggcg ccaatctctg agccgagctg
     4381 ccgttttcga cgggcaattc ccgggattcg tggcaccgga tccgccgcta gcgctacaga
     4441 tgcttttgcg cggcctgatt caacttcgct tcgaaagtct cctg
//
LOCUS       DASRZF010000214         7615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3240834, whole genome shotgun sequence.
ACCESSION   DASRZF010000214 DASRZF010000000
VERSION     DASRZF010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 7615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7615
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3240834"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="VFG22_09590"
     CDS             <1..642
                     /locus_tag="VFG22_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416537.1"
                     /translation="FHELGHAAASWLSSRIAIPLPFFTFWYDEQSILMGSLVAGGLGW
                     LMFHTYREKNWFMLSSASVLFLAWLVITFAFAPERTLMWQILAGALGEIVFGGFILVA
                     FHFPLPDRFRWDFWRWVALIPASLSFVHATMLWRTVAADVSQMPWGSAIGSEDDGDMN
                     RLVQQFHWSANALAEVYLTTAYFALAALAVAYAYAGYRYFVRNGGWPVFQRVR"
     gene            complement(635..985)
                     /locus_tag="VFG22_09595"
     CDS             complement(635..985)
                     /locus_tag="VFG22_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HET6416538.1"
                     /translation="MTWVITRLCRDCVDKSCVDVCPVECIYEYKGDDHKAFPNQLYIQ
                     PEECIDCGACEPECPWEAIYEDASVPEVFTEDTQLNAKMMENQDDFEVPVNDGTEQPT
                     PEQVAENKAKWGLT"
     gene            complement(1063..1593)
                     /locus_tag="VFG22_09600"
     CDS             complement(1063..1593)
                     /locus_tag="VFG22_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416539.1"
                     /translation="MKSVALGPAAFLLAASFLMACGGSKTSSSGSTGKVSAIEAMGIT
                     PPDSDWEQMSVADKEFYMIGKVNPIMKELFAGHDAEEFGDFDCVDCHGEEMREIDFKM
                     PAPSMYIVPPEGTPGHRGMLATFPDEVKFMQETVTPSMGKLLGIESFSCAGCHPSEAP
                     KKKAPATKRKPSKPKG"
     gene            1624..2415
                     /locus_tag="VFG22_09605"
     CDS             1624..2415
                     /locus_tag="VFG22_09605"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6416540.1"
                     /translation="MTAMFAQFQRQMIFPGPEGITVPLLNDVASEVGATELQIATGDG
                     ETLYGWHRAAIWSGPRRVVLYFHGNASSLVAQLGLQSRLLSEGWDFVGIHYRGYPGST
                     GVPSEAGLHEDAKAAWTWITSKLGVPPKQVAIHGRSLGGAVAVQLAAKVNPGALVMES
                     TFTSLVELAKEHYRWLPIGSLLAHRFMSRDFANRVACPVLVAHGSADSIVDVHHGREL
                     ARLFQAETYVEVPRIGHNDMLFVGTIAARYLGFLEAAIPPPRQSK"
     gene            2419..4344
                     /locus_tag="VFG22_09610"
     CDS             2419..4344
                     /locus_tag="VFG22_09610"
                     /inference="COORDINATES: protein motif:HMM:NF005511.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET6416541.1"
                     /translation="MFYFFPMAKGKKSLAPKPAEPPLILTKSNGDKWDAEAEVVVVGF
                     GGAGACAALEAHAEGARVLVLDRFHGGGATAISGGVFYAGGGTHIQREAGVSDDADEM
                     FRYLSLEVKGVVSEDTLRDFCDKSVANLDWLEAHGVPFEATLCPYKTSYPTDDYYLYY
                     SGNEGFSPYKDAAKPAPRGHRAKGAGLPGESFYEPLRESVVRAGIEVEYEARVTRLVI
                     DGDDRVLGVEYRQVEAGLWSKLYRRLHQAGIAIVKYNPKLAAKLRERCFRIEAEHSVT
                     KRVRAQKGVILAAGGFIYNRKMVNEIAPKYRSGMPLGTTSDNGSGILLGQSVGGKTDL
                     MNRISAWRFINPPEAFAKGIIINQKGERYINEFMYGAAVGEAMVEQNDGVAILVINGE
                     LKKLAREQCKPGKAQWFQRAPALLNLWFNSKEAKSVEELAKVARVPLESLRKTLDEYN
                     DAADGKITDRFSKDPEKMHAMRKGPYFAINAGIKSKRFPCPTLTLGGLVVDEQTGQVR
                     REDGGLVPGLYAAGRNAVGVCSRQYVSGLSIADCVYSGRRAGGAAARGDLYTAKNVPD
                     ATQTRAVEAKPLEGLPVLQESQVADPSPEAHERKGRRARTDAAYPRQALNRTIRRPGG
                     TTRVASSGRGRLSKRAT"
     gene            4386..4982
                     /gene="folE"
                     /locus_tag="VFG22_09615"
     CDS             4386..4982
                     /gene="folE"
                     /locus_tag="VFG22_09615"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HET6416542.1"
                     /translation="MTERIDEEEAAECVRKLLGYIGDDPEREGLLDTPKRVLGAIQEH
                     FRGYHEDPQEHLARTFTEVEGYDELVLISDIEIYSHCEHHVVPFVGKAHVAYIPNGKV
                     VGLSKIARVVDTFAKRLQVQEKLTAQIANAINETLSPQGTAVILQCQHFCMCYRGVRK
                     PGSWTTTSRLTGCFLEDEAARMELLTLIGMQRSIGGAG"
     gene            5027..6673
                     /locus_tag="VFG22_09620"
     CDS             5027..6673
                     /locus_tag="VFG22_09620"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HET6416543.1"
                     /translation="MREQHYDWVIIGSGFGGSVSAHRLTQKGYKVLVLEKGRRFSDDD
                     FPKTNWDVKRWMWNPALGLKGIFQMSFMKHMTVLHGVGVGGGSLVYANTLPTPKSAFF
                     QSGSWSNLRDWETELAPFYATAKRMLGATPNPLETKGDRVMREIAKDLGREEHMHPTE
                     VAVYFGEPNKNVPDPYFDGEGPERTGCNFCGACMTGCRIGAKNTLVKNYLYLAEKNGA
                     EVRAETEVTAIGPREGGGYRVTTKPTFSKSGADEVTADRVILSGGVMGTMPLLLQMKE
                     DPAGLPRLSERVGDFVRSNSESLIAVLSPKKGTNFSKGVAITSILHTDEHSHMEPVRY
                     GAGSGFFRTLLAPHAPGPTVLSRLWGGFAAFLRQPARWLRALFVKDLAKHSQVLLYMR
                     TLEGTLRMRLGREWRTGYRRSLVTKVDDPSQAPSAFMEEATDLAERFADKVEGVTTTL
                     MTETLLGVPSTAHILGGACMGDSAETGVIDENHEVFNYPGLYVIDGSAISANPGVNPS
                     LTITTLAERAMSLIPAKHARLSTHPSEKGSAGFSEALRAG"
     gene            complement(6648..7316)
                     /locus_tag="VFG22_09625"
     CDS             complement(6648..7316)
                     /locus_tag="VFG22_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416544.1"
                     /translation="MWRCLLIVLALGFGCAGRQTPDGSQETVVSPIPVPQPVYPREAL
                     SNDLQELWARVEEAVAVRPPEPPEGASAEVIEAWAEGAFRQWLLQRQAATDRALAATH
                     ALKTHPLFERGIGTALFGYMYEDMAGSIRGAPVPDEIAADEELLEIYTGALTEHITPF
                     AELSARAYYACVALFTKLDDPQWGEWAYYCDERGAEVVGTFKLQPPEPSDTNTTLTQL
                     STLR"
     gene            7405..>7615
                     /locus_tag="VFG22_09630"
     CDS             7405..>7615
                     /locus_tag="VFG22_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416545.1"
                     /translation="MVAPLVELTDAASVDRAARALFGAPLDTRPAVSHSFAAWRSEPD
                     APLTAIKINPQSPKSDLDFLALHIAR"
BASE COUNT         1439 a         2453 c         2371 g         1352 t
ORIGIN      
        1 tttcacgagc tcggccacgc agcagcgtcc tggctcagca gccggatcgc gatcccgctt
       61 ccgttcttca cgttctggta cgacgagcaa tcgatcctga tgggctcgct ggtcgccggc
      121 ggtctcggct ggctgatgtt ccacacctat cgcgagaaga actggttcat gctgagcagc
      181 gcctcggttc tgttcctggc ctggctcgtg atcaccttcg ccttcgcccc cgagcggacg
      241 ctgatgtggc aaatcttggc aggcgcgctc ggcgagatcg tcttcggcgg attcatcctc
      301 gtcgccttcc acttcccgct gccggatcgc ttccgatggg atttctggcg ctgggtcgcg
      361 ctgatccctg cgtctctgag cttcgtgcac gcaacgatgc tttggcgaac cgtcgcggct
      421 gacgtttcac agatgccctg gggctcggcc atcgggagtg aagatgacgg cgacatgaac
      481 cggctggttc agcagttcca ctggagcgcc aatgcacttg cggaggtgta cctcacgacc
      541 gcctattttg cgctggcggc cttggccgtc gcctatgcct acgcgggcta tcgctacttc
      601 gtacgaaacg ggggttggcc cgtgtttcag cgcgtcaggt gaggccccac ttggccttgt
      661 tctcggccac ctgctcaggc gtcggctgct cggttccatc gttcaccgga acctcgaagt
      721 cgtcctggtt ctccatcatc ttggcgttga gctgagtatc ctcggtgaag acttcgggga
      781 ccgatgcgtc ctcgtagatc gcttcccatg ggcactccgg ctcacaggca ccgcagtcga
      841 tgcactcttc gggctggatg tagagctggt tgggaaacgc tttgtggtcg tcgcccttat
      901 actcgtagat gcactcgacg ggacagacat caacgcagga tttgtcgacg cagtcgcggc
      961 acagacgtgt aatgacccag gtcatcggac tccctttcga gaaaaaagtg gctgaacgct
     1021 agcaggggca tctagctggg tcaaagatgg gcagcgacgc gactatccct tgggtttgga
     1081 cggcttgcgc ttggtcgcgg gcgccttctt cttgggcgct tcgctcggat ggcacccggc
     1141 gcagctgaag ctctcgatgc cgaggagctt gcccatcgaa ggcgtcaccg tctcctgcat
     1201 gaacttcacc tcgtccggga aggtggcaag cattccgcga tgcccgggcg tgccctcggg
     1261 cggaacgatg tacatcgagg gcgcgggcat cttgaaatcg atctcgcgca tctcttcgcc
     1321 gtggcagtcg acgcaatcga aatccccgaa ctcttcggca tcgtgaccgg cgaagagctc
     1381 cttcatgatc ggattcacct tgccaatcat gtagaattcc ttgtccgcca cgctcatctg
     1441 ttcccagtcg gaatcgggcg gcgtgatccc catcgcttcg atggcgctca ccttcccggt
     1501 cgagcccgac gacgaggtct tcgagccccc gcaagccatt agaaaagacg cagccagcag
     1561 aaatgcggca ggtccgagcg cgactgattt catgattcct gtcatcgcca cgcatggtaa
     1621 gtcatgactg cgatgttcgc acagtttcaa aggcagatga tcttcccagg gccggagggc
     1681 atcaccgtcc ccttgctgaa tgacgtcgcc tctgaggtgg gcgccaccga gctccagatc
     1741 gcaaccggcg acggcgaaac tctctacggt tggcatcggg ctgcgatctg gtccgggccg
     1801 cgacgcgtcg ttctctactt ccacggcaac gcctcctcac tcgtcgcgca gctggggctg
     1861 cagagccgct tgctgagcga gggctgggac ttcgtaggca tccactaccg cggttacccc
     1921 gggagcacgg gcgttccaag cgaggcgggg ctccacgagg atgcgaaagc tgcctggacc
     1981 tggatcacta gcaagctcgg cgttccgccg aagcaggtcg cgattcacgg acgaagcctc
     2041 ggcggggcgg tcgccgttca gcttgccgcg aaggtgaacc ccggcgcgct ggtaatggag
     2101 tccacgttca cttcgctggt cgagctagcg aaggagcact atcgctggct gcccatcggg
     2161 agcctcttgg cgcaccgctt catgagccgt gatttcgcca accgcgttgc atgcccggtg
     2221 ctcgtggcgc acggaagcgc ggacagcatc gtggacgttc accacggcag agagctcgcg
     2281 aggctctttc aagcggagac atacgtcgaa gtgccgcgga tcggccacaa cgacatgctc
     2341 ttcgtaggaa ccatcgctgc ccggtatctc ggcttcctgg aggcagcgat cccgccgcct
     2401 cgacagagca aatgaaacat gttctatttc ttccctatgg ccaaagggaa gaagtcgttg
     2461 gcgccgaaac cggcggaacc gccgttgatt ctcaccaaga gcaacggaga caagtgggac
     2521 gctgaagccg aggtcgtggt cgttggtttc gggggcgcgg gcgcatgcgc ggcgctcgaa
     2581 gcgcacgccg agggggcccg ggtcctggtc ctcgaccgct ttcatggagg cggtgccacg
     2641 gcgatcagcg gtggggtgtt ctacgcaggg ggcggcacgc acatccagcg cgaagccggc
     2701 gtgagcgacg acgccgacga gatgtttcgc tacctctcgc tcgaagtgaa gggcgtcgtc
     2761 tccgaagaca cactgcgcga cttctgcgac aagagcgtcg ccaacctcga ctggctcgaa
     2821 gcgcacggcg ttccgttcga agccacgctc tgtccgtaca agacgagcta tccgaccgac
     2881 gactactacc tctactactc cggcaacgaa ggcttctctc cctacaaaga tgccgccaag
     2941 ccggcgccgc gcggtcatcg ggcaaaggga gccgggcttc cgggcgagag cttctatgaa
     3001 ccgcttcggg agtccgtcgt gcgggcgggc atcgaagtcg agtacgaagc gcgggtcacc
     3061 cgcctcgtca tcgatggcga cgatcgagtc ctcggcgtcg agtaccggca agtcgaggca
     3121 ggcctctgga gcaagctcta ccgtcgactg caccaggccg gaattgccat cgtgaagtac
     3181 aacccaaagc tcgctgcaaa gctccgcgag cgttgcttca ggatcgaagc cgagcattcg
     3241 gtgaccaagc gcgtgcgcgc tcagaagggc gtgatcctcg ccgctggcgg tttcatctac
     3301 aaccgaaaga tggtcaacga gatcgctccg aaataccgct ccggcatgcc gcttggaacc
     3361 accagcgaca acgggagcgg catcctgctc gggcagagcg tgggtggcaa gaccgacctg
     3421 atgaaccgaa tctccgcgtg gcgattcatc aacccccccg aggcgttcgc caagggcatc
     3481 atcatcaacc agaaaggcga gcgctacatc aacgagttca tgtacggtgc agccgtcggc
     3541 gaagcgatgg tcgagcaaaa cgacggcgtc gccatcctcg tcatcaacgg cgagctcaag
     3601 aagttggcac gggaacagtg caagcccggc aaggcgcaat ggtttcagcg cgcgcccgcc
     3661 ctgctcaacc tctggttcaa cagcaaagag gcgaagtcgg tcgaagagct cgcgaaggtc
     3721 gcgagggtac cactcgaatc acttcgcaag acgctcgacg agtacaacga cgccgccgac
     3781 ggcaagatca cggatcggtt ctcgaaggac ccggaaaaga tgcacgcgat gcgaaagggt
     3841 ccgtacttcg ccatcaacgc ggggatcaag agcaagcgct tcccctgccc gacgctcacg
     3901 ctcggcggtc tcgtggtcga cgagcaaacc ggccaggtcc gtcgcgaaga cggcggactg
     3961 gttcccggcc tctacgcggc cggtcgcaat gccgtaggcg tctgctcgcg acagtacgta
     4021 agcggtttgt cgattgccga ttgtgtgtac tccggacgcc gcgcaggcgg cgccgcagct
     4081 cggggcgatc tctacacggc caagaatgtg cccgacgcga cgcagacccg cgcggtcgag
     4141 gcgaagccgc tcgaaggcct tccggtcctt caggagtctc aggtcgcgga cccttcaccc
     4201 gaggcccatg agagaaaggg ccgccgcgcg cggacggatg cggcctatcc gcgccaagct
     4261 ctgaatcgga cgattcggcg cccaggcggg accacgcgtg tggcatcatc gggtcgcggg
     4321 cggctttcaa agcgcgcgac ctgacttgac gccgcagggc tgcggcatga aggtggccaa
     4381 cggcgatgac tgaaagaatc gacgaagaag aagccgccga gtgtgtgcga aagctcctcg
     4441 gctacatcgg tgacgatcct gagcgggaag gtctgctgga cactccgaaa cgggttctcg
     4501 gcgcgattca agagcacttc cgcgggtacc acgaagaccc gcaggaacac ctcgcgcgca
     4561 cgttcaccga ggtcgagggc tacgacgagc tggtcctgat cagcgacatc gagatctaca
     4621 gccactgcga gcatcacgtc gtgccgttcg tgggcaaggc ccacgtcgcg tacatcccca
     4681 acgggaaggt ggtggggctt tcgaaaatcg cgcgcgtcgt ggacactttt gccaagcggc
     4741 tacaggtcca ggaaaagctc acggcccaga tcgccaacgc catcaacgag acgctcagcc
     4801 cgcaaggaac ggcggtcatt ctgcagtgtc agcacttctg catgtgctac cgcggtgtcc
     4861 gtaagcccgg gtcctggacg accacgagcc gactcaccgg ctgtttcctc gaagatgagg
     4921 ccgcgcggat ggagcttctc acactgatcg gaatgcagcg atccatcggc ggcgcgggct
     4981 agaaggccgg ctcgatctcg ccgcgatccg ggcgtagagt gagggcatgc gcgagcagca
     5041 ctatgattgg gtcatcatcg ggtcgggttt cggcggaagc gtatccgcac atcgcctgac
     5101 gcaaaaaggg tacaaggtcc tcgtcctcga gaagggacgg cgcttctcgg acgacgactt
     5161 ccccaaaacc aactgggacg tgaagcgctg gatgtggaac ccggcgctcg gtctcaaggg
     5221 cattttccag atgtcgttca tgaagcacat gaccgtgctt cacggcgtgg gcgtcggcgg
     5281 cggctcactg gtgtacgcca acaccttgcc cacgcctaag tcggcgttct tccaatcggg
     5341 gtcctggagt aacctccgcg attgggaaac cgagctcgcg cccttttacg cgacggcgaa
     5401 acgcatgctc ggcgccacgc ccaatccgct cgaaaccaag ggcgaccgcg tgatgagaga
     5461 gatcgcaaaa gacctgggcc gcgaagaaca catgcacccc accgaggtcg cggtctactt
     5521 tggcgagccc aacaagaacg tgcctgaccc atacttcgac ggtgaaggtc ccgaacgcac
     5581 cggctgcaat ttctgcggtg cctgcatgac cggctgccgc atcggcgcaa agaacacact
     5641 ggtcaagaac tacctctacc tcgccgaaaa gaacggcgct gaggtgcgcg cggaaaccga
     5701 agtcaccgct atcggaccgc gcgagggcgg cggctatcgg gttacgacca agccaacctt
     5761 cagcaagagc ggcgcggacg aggtcacggc cgaccgagtg atcctttccg gcggcgtcat
     5821 gggcaccatg ccgctgctcc tccaaatgaa ggaagacccc gcaggtctcc cgcgcctctc
     5881 cgagcgcgtc ggggacttcg tccgctccaa cagcgaatcg ctcatcgcgg tcctttcgcc
     5941 aaagaaggga accaacttca gcaagggcgt cgccatcacg tcgatcctcc acaccgacga
     6001 gcacagccac atggaaccgg ttcgctacgg cgccggctcc ggcttcttcc gcaccttgct
     6061 agcgccccac gcgccaggcc caacggttct ctcgcggctc tggggcggct tcgcggcctt
     6121 ccttcgccaa ccggcccgct ggctgcgcgc gctcttcgtc aaggacctcg ccaagcactc
     6181 tcaggtgctc ctctacatgc gcacgctcga aggcaccctt cggatgaggc tcgggcgaga
     6241 gtggcggacc ggctaccggc gctcgctggt caccaaagtc gacgacccca gccaggcccc
     6301 ttctgcattc atggaagaag ccaccgacct ggccgaacgc ttcgcggaca aagtcgaagg
     6361 ggtcaccacg acgctcatga ccgagacgct tctcggcgtc ccctcgaccg cacacattct
     6421 cggcggcgcc tgtatgggcg actcggccga gacgggcgtc atcgacgaga atcacgaggt
     6481 cttcaactac cccggcctct acgtcatcga cggctctgcg atttcagcga accccggcgt
     6541 gaacccatcg ctcaccatca cgacgctcgc cgaacgcgcg atgagcctca tccccgccaa
     6601 gcacgcaagg ctatccaccc atccttcgga aaaggggtcc gccggcttca gcgaagcgtt
     6661 gagagctggg tgagggtcgt gttggtgtcg ctcggctcgg gcggctggag cttgaacgtt
     6721 ccgacgacct cggcgcctcg ttcgtcgcag tagtacgccc attctcccca ttgcggatcg
     6781 tcgagcttgg tgaagagcgc gacgcaggca tagtaggcgc gcgccgagag ctcggcgaac
     6841 ggggtgatgt gttcggtgag ggcgccggtg tagatctcga gcagctcttc gtcggccgca
     6901 atctcgtccg ggactggagc gccgcggatg ctaccggcca tgtcttcgta catgtagccg
     6961 aagagcgcgg tgccaattcc acgctcgaac aggggatggg tcttgagggc atgcgttgcg
     7021 gcgagcgccc gatcggtcgc cgcttggcgc tgaagcagcc attgtcggaa cgcaccctca
     7081 gcccaggctt cgatgacctc ggccgaagcg ccctccggag gctcgggcgg gcgcacggcc
     7141 accgcctctt cgactcgcgc ccagagctcc tgcaagtcgt tgctgagggc ctcgcgcgga
     7201 tacaccggtt gtgggacggg aatcgggctg acgaccgttt cctgcgaccc atcgggggtc
     7261 tggcggccgg cacaaccgaa tcccagggcc agcacgatca agagacaacg ccacatccgg
     7321 cgagattaac aggcgcagat ctcgggggcg agttttggga tcaacgccgt tgacttgatc
     7381 gtcaatccac catttcatcg agccatggtt gcccctctcg tcgagctgac cgatgccgcc
     7441 agcgtcgacc gggcggcgag ggccttgttc ggggcacccc tcgacacgcg acccgcggtc
     7501 tcgcacagct tcgcggcctg gcgctctgaa ccggacgcgc cgctcactgc gatcaagatc
     7561 aacccgcaga gcccgaagag cgatctcgat ttcctcgctc tgcacatcgc ccgtg
//
LOCUS       DASRZF010000215         6741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1523292, whole genome shotgun sequence.
ACCESSION   DASRZF010000215 DASRZF010000000
VERSION     DASRZF010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 6741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6741
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523292"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VFG22_09635"
     CDS             complement(<1..260)
                     /locus_tag="VFG22_09635"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6416546.1"
                     /translation="MGLYENYILPPLIHLACGAKPNMRQREKVVPLAEGEVLEIGMGS
                     GLNLGYYDPRKVRKIWGLEPSPGMRQLARKNMGKVELDVELI"
     gene            complement(378..1448)
                     /locus_tag="VFG22_09640"
     CDS             complement(378..1448)
                     /locus_tag="VFG22_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416547.1"
                     /translation="MQKHFFNRFFELDPFLDDAEARSLVRLCEDFGSYGMYSEEGLNE
                     GIGEGLPQRFDAVFNFLRTGGRLGRRDADMGMLVARTNYFRETYAYGDEISASGIESL
                     YRNERLVEAAKQIFDRPIVEPAIVYANILLPGQELAVHTDVPEFRGLNRKLHPQWLIV
                     VAHHSGLFDEYRMPIATSVSWYQDTNGGEFAFYPDGSDGAARALDVHFNTALIMDTDS
                     VFHGVDRVLEISQPLPSLLPRMRLFPEGDGHWVVRDGEREIGRYEWDEMRFSISWKAY
                     CFRDAAERAAWRDHSRDLDAETVLDTLCDDLRARGRISDERPPNRELAEILVDEYVKF
                     PPVFNQPMRRQTHESTPKERGA"
     gene            1493..2623
                     /locus_tag="VFG22_09645"
     CDS             1493..2623
                     /locus_tag="VFG22_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416548.1"
                     /translation="MSVPSNLPRTWALLANGIILGFLASFAWLAVCEPDAYYRSVQED
                     QALEWASFWGFLIAGLAFGMVAFRQRRSIGALPWFPIGLALFCFFVAMEEISWGQRVF
                     GHQPPVYFLETNYQQELNLHNIAGTWLRLFVFRAIVLGYGVLLPLLARIPVARRVLER
                     LRIVPPPASLVPSMLAMFCVHLWYPWKFTGEVIECALGMGFLFAAIANASQLTPEPNR
                     NPSRWTLGLTTLIVALGFSTAWWSQNRSTDDHAKVALAEAETAALARDLIEAANARSK
                     QTITSCGLHKRLYTFVEGNERARSLEAGAFAALIARGLPSTRAEFYLDAWNSPYWIRD
                     RCDKKRGRRAVFVYSYGPNRIRDSSRWEILGDDVGAYILVEP"
     gene            2694..4691
                     /locus_tag="VFG22_09650"
     CDS             2694..4691
                     /locus_tag="VFG22_09650"
                     /inference="COORDINATES: protein motif:HMM:NF026213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HET6416549.1"
                     /translation="MRAWSRSILILSLTLGACAPEQAAPSFDGADLPADVAGNTAPSA
                     TTAAFNAAFGARLPLSDQQDFEDARRGLVATDPELRVEGSDGHLIWDMPAYGFIQGAS
                     PDSVNPSLWRQAQLNNIHGLFEVTKGVHQLRGFDLANMTLIEGKTGWIVVDPLTSEET
                     ARAAIAFAREHLGNKPVSAVIFTHSHIDHFGGVLGVLTPDEASERGVPIIAPELFIDE
                     ATSENIVAGVAMSRRALFMYGFPLPRTVRGHVGSGLGKAPANGTFGILQPTQTISKTT
                     TDLTIDGVRFVFQYAPESEAPTELTFYLPDLKAFCGAEVVSHNMHNLYTLRGAKVRDA
                     IKWSGYIDEIIELFGDAEVYFGSHHWPIWGNARIIDFLEKQRDTYKFINDQTLRLALL
                     GFTPREIADQLELPESLRTTFSNRGYYGTIKHNAKAVYQRYFGWYDGNPANLDPLPPQ
                     EAGKKYVELAGGAAKLIENAQTAYDAGEYRWAAEVLNHLVFADPENRDARELLARVYD
                     QMGYQAESGPWRDVYLTGAFELRHGGPKEGINLASAKDMIKHAPLERFLDAMAARLDG
                     AKAEGKEMILNLVITDLGTTHVLELKNSVLRHYERDARSDANATMKISHALLLRLLTG
                     TAGLKDTLFSDDLKIEGSRLDLLGFFRLFDKPEGTFNIVTP"
     gene            4697..5119
                     /locus_tag="VFG22_09655"
     CDS             4697..5119
                     /locus_tag="VFG22_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416550.1"
                     /translation="MHEMFAFARIPSFAALCVLLATACTEQGNSCPQAPGLIAQGVVR
                     FSPSDVDCEEIPSSMALLEPMASRGPVPADWPTQAVLSTEGETHRAKIAIASGTSLYG
                     TGEIAGPILRNGFVTQTWGVQPYRHRLHGRVSHFHVRR"
     gene            5058..6089
                     /locus_tag="VFG22_09660"
     CDS             5058..6089
                     /locus_tag="VFG22_09660"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HET6416551.1"
                     /translation="MCSPIDIDYMDGFRIFTFDDEKFPDPSGLNEYLHGIGFKSVWIL
                     DPGVKVEPGFAVYDEGLAGDHFIRKPDGAVFTGGSWPGDSVWPDYTKPETRAWWQSYI
                     PDFLDNGIDGIWIDLNEPSIVFPLGAPYPEDLLHAGGGDLPPDSHARYHNAYGKLMSQ
                     ATHEGMKLARPERRPFLLSRSNYLGGQRYAAMWTGDNSASWDHLRWSATMTLTMGLSG
                     QPFAGPDIGGFFRAPSDSLYAHWIGVGAFFPFSRTHTIQLSPPQEPWAFGPEVEAISR
                     AAIERRYRMLPYIYTLFRQSSLNGLPVWRPVFFADPSNRSSPKAIHTRSRHPLGSGAS
                     SPWWTRTRP"
     gene            6086..6454
                     /locus_tag="VFG22_09665"
     CDS             6086..6454
                     /locus_tag="VFG22_09665"
                     /inference="COORDINATES: protein motif:HMM:NF028447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5110 domain-containing protein"
                     /protein_id="HET6416552.1"
                     /translation="MTPELPVLKVRGGAIVPLGRVVQSTTEALLEPLTLLVSLDANGR
                     AQGVLYEDAGDGYGYQNGDYLFTTYSAEQAEGEVRVRIESEEGDRARPERTVAVIVIT
                     DDGELEGSGSETSTITVPLQ"
     gene            complement(6435..>6741)
                     /locus_tag="VFG22_09670"
     CDS             complement(6435..>6741)
                     /locus_tag="VFG22_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HET6416553.1"
                     /translation="DQIGGTAHDNLLATLGNVSVTAHILGGCVIGAGPDEGVIDVDHQ
                     VFGYPGLFVTDAAAIPANLGVNPSLTITALAERFSARFPSKEAANRERAHDATEAEP"
BASE COUNT         1302 a         2116 c         1981 g         1342 t
ORIGIN      
        1 tcgatcagtt cgacgtcgag ctccactttg cccatgttct tgcgcgccag ctgacgcatg
       61 cccggcgacg gctcgagtcc ccagattttt cgaaccttcc gggggtcgta gtaaccgaga
      121 ttcagaccac tgcccatgcc gatttcgaga acctcgccct ccgcgagcgg cacgaccttt
      181 tcgcgctgcc tcatattggg tttggctccg caagcaaggt ggatcagggg tggaaggatg
      241 tagttctcgt agaggcccat cttgtagaaa cgtaccagct ccccgcgcta cgcgccgccc
      301 ccagctgcct cgctccaaaa aggctggcgc tcaccgtcgt tactggataa cgtcgtcgcc
      361 ttctcggagg gagaggctca tgctccgcgt tccttcggcg ttgattcatg ggtttgccgc
      421 cgcattggct ggttgaaaac cggtgggaac ttcacgtatt cgtcgaccag gatctccgcg
      481 agctcacggt tcggaggccg ttcgtcgctg atgcgcccgc gcgcccgaag atcgtcgcag
      541 agcgtatcga gcaccgtctc cgcatccagg tccctgctgt gatcccgcca agcggctcgc
      601 tccgcagcgt ctcggaagca gtatgccttc caggagatcg agaatctcat ctcgtcccac
      661 tcatagcggc cgatctcccg ttctccgtcc cgaacgaccc aatggccatc tccctcggga
      721 aacagacgca tgcgcgggag aaggctcggc aagggctgcg atatctcgag gacgcgatcg
      781 acgccgtgga agacgctgtc ggtatccatg atgagcgctg tgttgaaatg cacgtcgagc
      841 gctctggccg cgccgtcgct tccgtctggg tagaatgcga attccccacc gttcgtgtct
      901 tgataccagg acaccgaagt tgcgatcggc atgcggtact cgtcgaagag gcctgaatga
      961 tgcgcgacga cgatgagcca ctgtggatgc agctttcggt tcaaaccgcg gaactctgga
     1021 acatccgtgt gcaccgcgag ctcctgacct ggcagcaaga tgttcgcata gacgatggcc
     1081 ggttctacga tcggacgatc gaagatctgc ttggcggctt caacgagtcg ctcgttgcgg
     1141 tagagcgatt ctatccccga tgcggaaatc tcgtccccgt aagcgtatgt ctcccgaaaa
     1201 tagttcgtgc gggcaacgag catgcccatg tcggcgtcgc gacgccccag tcgaccgccg
     1261 gtacgtagga agttgaatac ggcatcgaag cgctggggca gaccttcgcc gatgccctcg
     1321 ttgagtccct cttcggaata catgccgtaa ctgccgaagt cctcgcagag ccgaacgagc
     1381 gaccgcgcct cggcgtcatc gagaaaggga tcgagctcga agaagcgatt gaagaagtgc
     1441 ttttgcatag gcgaagaccc gttcgcggca tctgttgtac catgaaggac cagtgtctgt
     1501 tccctcgaac cttccccgca cctgggctct ccttgccaac gggatcatcc tcggctttct
     1561 ggcgtcgttc gcctggctcg cggtttgcga accggacgcc tactaccgca gcgttcaaga
     1621 agaccaggct ctcgaatggg cttcattctg gggcttcctg atcgccggcc tggcgtttgg
     1681 gatggtcgcg ttccgtcagc gtcgcagcat cggcgccctg ccctggttcc caatcgggct
     1741 cgcgcttttc tgcttcttcg tagcgatgga ggagatctcc tggggccagc gggttttcgg
     1801 ccatcagcca ccggtatatt tcctcgaaac gaactatcaa caggagctca acctgcacaa
     1861 catcgcgggc acgtggcttc gcttgttcgt atttcgcgcg atcgtgctgg gctacggggt
     1921 cctgctgccc cttctcgccc ggattcccgt ggcacgccga gtgctggagc gccttcgtat
     1981 cgtacctcca ccggcctcgc tcgtgccttc catgttggcg atgttctgcg ttcacctttg
     2041 gtacccatgg aagttcacgg gcgaagtcat cgaatgcgcg cttggaatgg gctttctgtt
     2101 tgccgctatc gcgaacgcgt ctcaactgac gcccgaaccc aatcgaaacc cttcacgctg
     2161 gaccctcggg ctcacgactc tgatcgtcgc cctcggcttt tcgacggcct ggtggtctca
     2221 gaatcggagc accgatgacc atgccaaagt cgcgctcgcc gaagcagaga ccgcggctct
     2281 ggcacgcgac ctcatcgagg cggcgaacgc gcgtagcaag caaacgatca cgagctgtgg
     2341 cttgcacaag cgtctgtaca ctttcgtaga gggaaatgag cgtgcgcgat ctctcgaggc
     2401 aggtgccttc gcggccctga tcgcgcgcgg gcttcccagc acgcgtgcag agttctatct
     2461 cgacgcttgg aattcaccgt attggatccg cgatcgatgc gacaagaaga ggggaaggcg
     2521 ggcggtgttc gtatactcgt atggtccgaa ccggatccgg gactcgagcc gttgggaaat
     2581 cctcggtgac gacgtaggcg cgtacatact cgtcgagccc tgacgcgcag catttgccgt
     2641 tccaaatgcg ctcggtttcc cgtaggctcc ctccgtcgag gagggaaatg cccatgcgag
     2701 cttggagccg ttcgattttg atcttgtcgt tgacgctcgg agcctgcgct cccgagcagg
     2761 ccgcaccgag cttcgacggc gcagatctgc cggcggacgt cgcgggcaac acggcgccct
     2821 ccgcaacgac ggccgcattc aacgcggcgt tcggcgcacg cctccccctc tccgatcaac
     2881 aggacttcga agacgcgagg cgcggcctgg ttgcgaccga tcccgagctt cgcgttgaag
     2941 gctccgacgg tcacctcatt tgggacatgc ccgcatacgg tttcatccaa ggcgcctccc
     3001 ccgatagcgt gaacccgagc ctctggcgcc aagcgcagct caataacatt cacggcttgt
     3061 tcgaagtcac caaaggggtg catcaacttc gcggcttcga cctcgcgaac atgacgctga
     3121 tcgaaggcaa gaccggatgg atcgtcgtcg acccgctgac ctcggaagaa acggcccgtg
     3181 ccgcaatcgc cttcgctcgc gagcaccttg gaaacaaacc agtatccgcg gtcattttca
     3241 cgcatagcca catcgatcac ttcggtggcg tgctcggggt tctcacccca gacgaagcaa
     3301 gcgagcgcgg ggttccgatc atcgcgcctg agctcttcat cgacgaggcg accagcgaga
     3361 acatcgtcgc aggcgtagcg atgtcgcgac gcgcgctctt catgtatgga tttcccttgc
     3421 ctcgcacggt ccgcggtcac gtcggttccg gtcttggaaa agcacccgct aatgggacat
     3481 tcggcatcct tcaacctacc cagacgattt ccaagacgac gactgacctc acgatcgacg
     3541 gcgtgcggtt cgtgtttcag tacgcaccag agtcagaggc gcccaccgag ctgaccttct
     3601 acctgccgga tctcaaggct ttctgcggcg ctgaagtggt gtcgcacaac atgcacaacc
     3661 tgtacacttt gcgtggcgcc aaggtccgtg acgcgatcaa gtggagtggt tacatcgacg
     3721 agattatcga gctgttcggt gacgccgagg tctactttgg tagtcaccac tggccgatct
     3781 ggggcaatgc tcgcatcatc gacttcctcg agaagcagag agacacgtac aagttcatca
     3841 atgatcaaac actgcggctc gcactgctcg gcttcacccc gcgtgagata gccgatcagc
     3901 tcgagctacc ggagtcgctc cgaacgacgt tttcgaatcg gggctactac ggcaccatca
     3961 agcacaatgc gaaggccgtc taccaacgct acttcggctg gtacgacgga aaccccgcaa
     4021 atctcgaccc gcttccccct caggaagcag gcaagaagta cgtggagctt gctggcggcg
     4081 ccgccaagct gatcgagaac gcacagaccg cctacgacgc tggagagtat cgctgggccg
     4141 cggaggtgct gaaccacctg gtctttgcgg atccagagaa tcgtgacgcg cgcgagctac
     4201 tggcccgcgt ctacgatcaa atggggtacc aggcggaatc agggccctgg cgtgacgtct
     4261 atttgaccgg cgctttcgag ctccggcacg gagggcccaa ggaaggaatc aacctcgctt
     4321 cggcgaaaga catgatcaag catgcaccct tggagcgatt tctcgatgcg atggccgcac
     4381 gcctcgacgg tgccaaggcc gaaggcaaag agatgattct gaacttggtg atcacggacc
     4441 tcggaaccac ccacgtgctg gagctcaaga actcggtgct gcgccactac gaacgcgacg
     4501 cgcgatccga cgcgaatgca acgatgaaga tcagccatgc gctcttgctg cgattgctga
     4561 ccggcaccgc aggcctcaaa gacaccctct tctcggacga cttgaagatc gagggaagcc
     4621 gactcgactt gctcgggttc ttccgcctct tcgacaaacc cgagggcacg ttcaacatcg
     4681 tcaccccata gaaccaatgc acgagatgtt tgcgttcgct cgaatccctt cattcgcagc
     4741 gctatgcgtg ctcctggcca ccgcgtgcac ggagcaaggc aacagctgtc cgcaagcccc
     4801 gggcctgatc gcacaaggcg tcgtccgctt cagcccgtcg gacgtcgact gcgaagagat
     4861 tccaagctcg atggctcttc tcgaacccat ggcgtcccga ggtcccgtcc ctgcggactg
     4921 gcccacgcaa gcggtactca gcaccgaagg tgaaacgcat cgagcgaaga tcgcgatcgc
     4981 tagcggcacc agcctctacg gaactggaga gatcgccggt ccgatcctga gaaacggctt
     5041 cgtcacgcag acctggggtg tgcagcccta tcgacatcga ctacatggac gggtttcgca
     5101 ttttcacgtt cgacgatgag aagtttcccg atccgagcgg attgaacgag tacctgcacg
     5161 gaattggctt caagtcggtt tggattctgg acccgggagt gaaggtcgag cccggtttcg
     5221 ccgtgtacga cgagggcctc gcaggtgacc acttcattcg caaaccagac ggcgccgtat
     5281 tcaccggcgg aagctggccc ggggattcgg tctggccgga ctacacgaaa cccgagaccc
     5341 gtgcctggtg gcagagctac attccggatt tcctcgacaa tgggatcgac ggcatctgga
     5401 tcgatttgaa cgaaccttcg atcgtcttcc cgttgggcgc gccctatccc gaggatctct
     5461 tacacgcggg cggcggcgac ctgcctccgg attcgcacgc gcgctaccac aacgcctacg
     5521 gcaagctcat gtcgcaagcg acccacgagg gaatgaagct ggcgcgaccc gaacgaaggc
     5581 ccttcttgct ctcgcgctcc aactatcttg gcggacagcg atacgccgcg atgtggaccg
     5641 gggacaattc agcgagctgg gatcacctac gatggtcggc gacgatgacg ctgacgatgg
     5701 ggctctccgg gcaaccgttt gcaggaccgg acatcggagg gttcttccgc gcaccgagcg
     5761 actcgctcta cgcgcactgg atcggcgtcg gggccttctt tccgttcagc cgtacacaca
     5821 ccattcagct ttcacctcct caagagccct gggcgttcgg gccagaggtc gaagcgattt
     5881 cgcgggcagc catcgagcgt cgataccgaa tgctcccgta catctacacc ttgttccgtc
     5941 aatcctcgct caacggcttg ccggtttggc gccctgtgtt cttcgcggat ccgtcgaacc
     6001 ggtcctcacc gaaggcgatc cacactcgtt ccagacaccc gctgggatct ggcgccagtt
     6061 caccttggtg gacgaggacc cggccgtgac tcccgagctt ccggtcttga aggttcgcgg
     6121 aggagcgatc gtacctttgg gtcgtgtcgt gcaaagcacc acggaggccc tactcgaacc
     6181 gctcactttg ctggtgtccc tcgatgccaa cggtcgagcg cagggcgtgc tctacgaaga
     6241 cgccggagac ggttacggct accagaacgg cgactacctc ttcacgacct actcggccga
     6301 gcaggctgaa ggggaagttc gggtccgaat tgaatccgaa gaaggcgacc gtgcacgtcc
     6361 cgaacgcacc gtggcggtga tcgtaatcac ggacgatggg gagcttgaag ggagcggttc
     6421 ggaaacctcg acgatcacgg ttccgcttca gtagcgtcgt gcgctcgttc acggttggcg
     6481 gcttccttgg atggaaatcg cgcgctgaat cgttccgcca gggccgtgat ggtgagtgag
     6541 ggattgacgc ccaaatttgc ggggattgca gccgcgtcgg ttacgaaaag ccccgggtat
     6601 ccgaagacct gatgatcgac gtcgatgacc ccttcgtcgg gacccgcacc gatcacgcaa
     6661 ccgccgagaa tgtgggcggt gaccgagacg tttcccaacg tcgcaagcag gttgtcatgc
     6721 gccgttcctc ctatctggtc c
//
LOCUS       DASRZF010000216         2439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2553212, whole genome shotgun sequence.
ACCESSION   DASRZF010000216 DASRZF010000000
VERSION     DASRZF010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2553212"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VFG22_09675"
     CDS             complement(<1..279)
                     /locus_tag="VFG22_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416554.1"
                     /translation="MQYPSPDQPFAVKAINRVGAFARRIGIGASGFRAEDLMHEAAKK
                     TGLSDFGDEGFIPGLQILVESLNEEARLSTIGRIGAKDMLLTCLSNRLR"
     gene            complement(358..948)
                     /locus_tag="VFG22_09680"
     CDS             complement(358..948)
                     /locus_tag="VFG22_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416555.1"
                     /translation="MNLQMKIKMASMLFAIATCFADGSALAQDASKGEAPKLFADLSS
                     STRVYPNANAALTGFALGSTYKPTEKCLHIAGELFARFGRKDGTPDDLKMRTAGGAIG
                     ALWGTGDWDHWFGAGAMFDLGWGRVGNQDEFVSSAYLRALLLWRLRGEFWVNLDLRTG
                     YVIAPITVAGVGIKGPLLGLGIGVTWGKDEDEYAPK"
     gene            1041..1931
                     /locus_tag="VFG22_09685"
     CDS             1041..1931
                     /locus_tag="VFG22_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416556.1"
                     /translation="MNLKIRHLWPLLGFVLLVSRTVAAQDDLASDATPDPVAEDAIES
                     GWTYPQSYSIRPLTMQKGMVRANGIFTVWDDGVDTNIGITLGGSISVTDDIELGLNNY
                     RIGSSLQKNYEGLVPVVFYPSAGYGNIPIYGRYRFLHLDKLDFAGDLILVMPIDTPFA
                     LEVALPFRIKPNDRLSVDTGLEVRGTFGSAKYADIRIPAIVNYNFSDHAFIAGETGVQ
                     FINLGSSYGTAPNDTKGVAIPVGFRAGGTWKKPDKHMIDLFAGFSFPTLFEIGTSRST
                     VDAGIWDIFVGVAFYTRPLF"
     gene            1996..>2439
                     /locus_tag="VFG22_09690"
     CDS             1996..>2439
                     /locus_tag="VFG22_09690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HET6416557.1"
                     /translation="MPGRLLPRDFYARDALDVARDLLGQEIWHGPVGLRITEVEAYRY
                     PDDSANHCRAGVTPRNKPMWGPPGHAYVYLCYGMHQMLNLVTNQDGEGAAVLIRSCEP
                     IEGHRTIRRRRGGKQGPVLLTGPGKVGQALGLDPGFSGHPLYRRGG"
BASE COUNT          475 a          782 c          721 g          461 t
ORIGIN      
        1 gcgaagccga ttgctcaggc aggtgagcaa catgtccttg gccccgatcc gcccaatcgt
       61 cgagagacgg gcctcctcgt tgagcgattc gaccaagatt tgaagcccgg gaatgaaccc
      121 ttcgtcaccg aagtcggaaa gtccggtctt cttggcggcc tcgtgcatca gatcttcggc
      181 gcggaacccg gaggctccaa taccgatccg gcgcgcgaag gcgcccactc gattgatcgc
      241 cttcaccgcg aacggctggt ctggggacgg atactgcatc tagggcgcag gtatacatcg
      301 ccattgcgga cgaggcgaag gttgcccgcg agtcaggcgt ctccatgggg gagcagccta
      361 tttcggcgcg tactcgtctt cgtccttgcc ccaggtgacc ccgatcccga ggcccagcag
      421 gggccccttg attcccacgc cggcaacggt gatgggcgcg atcacatacc cggtccgcaa
      481 atcgaggttc acccaaaact cccctcgcaa tcgccagagg aggagggccc gcaaatacgc
      541 gctggacacg aattcatcct ggttgcccac gcgaccccag ccgaggtcga acatcgcgcc
      601 ggccccgaac cagtggtccc agtcacccgt accccagagc gcaccgatgg ctcccccggc
      661 ggtgcgcatc ttgagatcgt cgggcgttcc atccttgcgc ccgaagcgcg caaagagctc
      721 gcccgcgatg tgaaggcact tttcggtcgg cttgtaggtc gaccccaacg cgaacccggt
      781 gagcgcggca ttcgcattcg ggtacacgcg cgtgctcgag ctgagatcgg caaagagctt
      841 cggcgcttcg cccttggaag catcctgggc cagcgcgctt ccgtcggcga agcacgtggc
      901 gattgcgaag agcatggacg ccatcttgat tttcatttgc aagttcatgg gtcctccgtc
      961 cgaaccttcg ggatcttgaa ttcactttag cgccaaccga ccccagattg acagcactga
     1021 gcgaaggcgt aggtaccatc atgaacctaa aaatccgaca tctgtggcca ctgcttgggt
     1081 ttgtcctgct ggtaagccga acggtcgccg cgcaagacga cctggcctcc gacgcgactc
     1141 ccgacccagt cgcagaggat gcgatcgaaa gtgggtggac ctatccacag agctattcga
     1201 tccgccctct caccatgcag aagggaatgg tccgcgcgaa tgggatcttc acggtctggg
     1261 atgacggggt cgacacgaac atcggcatca cactcggcgg gtcgatcagc gtgaccgacg
     1321 acatcgagct tggcctgaac aactatcgga tcggctcctc gttgcaaaag aactacgagg
     1381 gactcgtccc ggtggtcttc tatccctcgg caggctacgg caacattccg atctacgggc
     1441 ggtatcgatt cctgcacctc gacaagctcg acttcgcagg cgacctcatt ctcgtgatgc
     1501 cgatcgatac gccgtttgcg ctcgaagtcg ccttgccatt ccgaatcaag ccgaacgacc
     1561 gactctccgt cgacaccggg ctcgaggtgc gcggcacctt cggttctgcg aagtacgcgg
     1621 acattcgaat tccggccatc gtgaactaca acttctccga ccacgcattc atcgctggtg
     1681 agactggggt gcagttcatc aatctcggaa gcagctacgg cacggcgccc aacgatacca
     1741 aaggcgtagc gatccccgtc ggctttcgtg ccggcggcac ttggaagaag cccgacaagc
     1801 acatgatcga tctgtttgcg ggattctcct tcccgacgct attcgaaata ggcaccagtc
     1861 gcagcaccgt cgacgccgga atttgggaca tcttcgtcgg cgtcgcgttc tacacgcgtc
     1921 cactcttcta gacctctgcg actcgctcga gcagggccat tggcaccggc acgattgctg
     1981 agacaaactt cagccgtgcc gggccgcctt cttcctcgcg atttttatgc acgcgacgcc
     2041 ctagacgtcg cgcgggactt gctgggtcag gagatctggc acggccccgt gggcctccga
     2101 atcaccgagg tcgaggctta tcggtatcca gacgacagcg cgaaccattg ccgtgcgggc
     2161 gtcacaccga gaaacaaacc gatgtggggg cctccgggcc acgcctacgt gtatctgtgt
     2221 tacggcatgc atcagatgct gaacttggtg accaatcagg acggggaggg tgcagccgtc
     2281 ttgatccgct cctgcgagcc gatcgaaggg caccgcacga tccgccgtag acgcgggggc
     2341 aagcaaggtc cggtgcttct caccggtccg gggaaggtcg gccaggcgct cggactcgac
     2401 ccgggcttca gtgggcatcc cttgtatcgc cgcggtggg
//
LOCUS       DASRZF010000217         2649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4958933, whole genome shotgun sequence.
ACCESSION   DASRZF010000217 DASRZF010000000
VERSION     DASRZF010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4958933"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /gene="rplU"
                     /locus_tag="VFG22_09695"
     CDS             <1..363
                     /gene="rplU"
                     /locus_tag="VFG22_09695"
                     /inference="COORDINATES: protein motif:HMM:NF013025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HET6416558.1"
                     /translation="AATSRLGALETQGLTDSSMYAVIQTGGKQYRVAEGDTIRVEKLA
                     GDVGSKVEFNDILMVGGETVAVGKPHVGGAKVTAEIVAQDLAKKIIVFKMKRRKGYRN
                     RRGHRQPYTELRIESVKG"
     gene            373..633
                     /gene="rpmA"
                     /locus_tag="VFG22_09700"
     CDS             373..633
                     /gene="rpmA"
                     /locus_tag="VFG22_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HET6416559.1"
                     /translation="MAHKKGQGSSRNGRDSNAQYRGVKVFGGQKVPAGSILVRQVGQT
                     FHPGANVGVGKDYTLWAKVDGVVTFERRGRTGKRVAVYPADN"
     gene            642..2129
                     /locus_tag="VFG22_09705"
     CDS             642..2129
                     /locus_tag="VFG22_09705"
                     /inference="COORDINATES: protein motif:HMM:NF017568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IG family 5'-nucleotidase"
                     /protein_id="HET6416560.1"
                     /translation="MQERFPAPEFDSPSGSAFPPPARRRVYCSRNLRLDQVKAVGFDM
                     DYTLAIYRQAEMDRLSIEATVAKLVDRGYPEELLGMKFRTDFPIRGLLIDRKLGNVLK
                     MDRFRYVKTAYHGFRKLSREERRGAYHTRRLRPGTGRYHWVDTLYSLSEVAVYAAVIE
                     HLEGRGNTLDYGRLFADIRECADLSHQDGSILDMVLEDLPRYVKRDPDLGQLLHKLRS
                     AGKRLFLLTNSGPEYSDAMMSFLLDDALGEYPSWKNYFDFICTFSKKPTFFTGKSPFI
                     ELESGREIRELSRGRVYTGGNIVDLERTLGVAGDEVLYVGDHIYGDVLRAKKESSWRT
                     AMIIQEMEDELRVLREHALSFERADSLEQTRDAVQDELREHQARLKRVERKLGEHESG
                     RERAKFEAKRVRHRRSIDRLRAQLKDLDAERAALSDVLDEAFHPFWGSVFKAGNEVSS
                     FGNQIEQYACLYTSRATNLSQYSPMHYFQSPRHRMPHEIGSVPGA"
     gene            2202..>2649
                     /locus_tag="VFG22_09710"
     CDS             2202..>2649
                     /locus_tag="VFG22_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416561.1"
                     /translation="MAKAAAKKSKKRATKKKSASKKNVKKSAKKAAPKKARKKAAKKA
                     AKKAAKKAAPKKAARKAAKKKTVKSEAANLKKKAKKRFTKRKKQATKAIAKFEKKFEK
                     TIDEAVAALKPKTSTKKQATKAIADIEKKIDEAVAALKPKTKTKKKP"
BASE COUNT          627 a          792 c          812 g          418 t
ORIGIN      
        1 gccgcaacct ctagactagg cgcccttgaa acccagggat tgacggattc atctatgtac
       61 gcggtcattc aaacaggcgg aaaacagtat cgggttgccg aaggcgacac gattcgcgtc
      121 gaaaagctgg caggcgatgt cggctccaaa gtcgagttca acgacattct gatggtcggt
      181 ggcgagaccg tcgcggtggg caagccgcac gttggcggcg caaaggtcac ggccgagatc
      241 gtcgcgcagg acctcgccaa gaagatcatc gtcttcaaga tgaagcgccg aaagggctat
      301 cggaaccgtc gcgggcaccg gcagccgtac accgagctcc gcatcgagag cgtgaaaggc
      361 tgagaaggcg ccatggcaca caaaaaaggt cagggctcat cccgtaacgg tcgcgattcc
      421 aatgcgcagt accgtggcgt caaggtcttc ggtggccaga aggttcccgc gggcagcatc
      481 ctggttcgcc aggtgggcca gacgttccac cccggcgcaa acgtgggcgt gggcaaagac
      541 tacacgctct gggccaaggt ggacggtgtg gtgacgttcg agcgtcgcgg ccgtaccggc
      601 aagcgcgtcg ccgtctatcc cgcagacaac tgagcgactc cttgcaggag cgttttccgg
      661 cgcccgagtt cgactccccg tccgggagcg cgttcccccc acctgcgcgc cgtagagtct
      721 actgcagtcg caacttgcgt ctcgaccagg tcaaggccgt tggcttcgac atggactaca
      781 cgctagcgat ctatcgacaa gccgagatgg atcggctgag catcgaggca acggtcgcga
      841 agctggtcga caggggctat cccgaggagc tcctcgggat gaagttccgc accgacttcc
      901 cgatccgcgg cctgctgatc gatcggaagc tcggcaacgt gctgaaaatg gatcgattcc
      961 ggtatgtgaa gacggcgtat cacggattcc ggaagctcag ccgggaagag cgccgcggcg
     1021 cgtatcacac ccgacgtctc cgccctggaa ccggtcgcta ccactgggtc gacacgctgt
     1081 actctctttc cgaggtggcg gtctacgccg cggtcatcga gcacctcgaa gggcggggca
     1141 acaccctcga ctacggcagg ctcttcgccg acattcgcga gtgcgccgac ttgtcgcacc
     1201 aggatggctc cattctcgat atggtcctcg aggatttacc gcgctacgtg aagcgcgacc
     1261 cggacctcgg ccagctcctt cacaagctgc gaagcgccgg taagcgactc ttcctgctga
     1321 ccaactccgg ccccgagtac agcgatgcga tgatgtcgtt cctgctggat gatgcgctcg
     1381 gcgagtaccc gagctggaag aactacttcg acttcatctg cacgttctcc aagaaaccca
     1441 ccttttttac ggggaaatcg cctttcatcg agctggagag cggccgcgag atccgggagc
     1501 tgtcgcgggg ccgagtctac accggaggca atatcgtcga cctcgagagg accttgggcg
     1561 tcgcgggaga cgaagtgctc tacgtgggcg accacatcta cggcgacgtg ctccgcgcca
     1621 agaaggagtc cagctggcgc accgccatga tcatccagga gatggaagac gagctcaggg
     1681 tgcttcgtga gcatgcgctg agcttcgaac gggcagactc cctcgagcag acgcgagatg
     1741 cagttcaaga cgagctgcgc gagcaccagg ctcggctcaa gagggtcgag cgcaagctcg
     1801 gcgagcacga atccggccgg gaaagagcca aattcgaggc aaaaagggtc cgtcatcggc
     1861 gatcgatcga ccggctccgc gctcagctca aggacctcga cgcagaacgc gccgccctga
     1921 gcgatgtcct cgacgaagcg tttcatccgt tctggggttc ggtcttcaag gcaggcaacg
     1981 aggtgtcgag cttcggcaat cagatcgagc agtacgcctg tctctacacc tcgcgtgcga
     2041 ccaacctctc gcagtactcg cccatgcact acttccagag cccgcgacat cggatgccgc
     2101 atgagatcgg cagcgtcccc ggcgcctagc cgcatctttt tttctacgca tttcgcgctt
     2161 cgcctgtcta tactgcgcac tcactcacaa cggagcgtgc aatggcgaaa gcagccgcaa
     2221 agaaatccaa aaagcgggcg acgaagaaaa agtcggcgtc caaaaagaac gtgaagaagt
     2281 ccgccaagaa ggcagcgccg aagaaggcgc gcaagaaggc agcaaagaag gcagcaaaga
     2341 aggcagcaaa gaaggcagcg ccgaagaaag cagccaggaa agcggccaag aagaagactg
     2401 tgaagtctga agccgccaac ctgaagaaga aggcaaaaaa gaggttcacg aaacgcaaga
     2461 agcaggccac caaggcgatc gccaagttcg aaaagaaatt cgagaagacg atcgacgagg
     2521 cggttgcggc gctgaagccc aagacctcga ccaagaagca ggccaccaag gcgatcgccg
     2581 atatcgaaaa gaaaatcgac gaggcggttg cggcgctgaa gcccaagacc aaaaccaaaa
     2641 agaagcccg
//
LOCUS       DASRZF010000218         5665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2901100, whole genome shotgun sequence.
ACCESSION   DASRZF010000218 DASRZF010000000
VERSION     DASRZF010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2901100"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..1312
                     /locus_tag="VFG22_09715"
     CDS             104..1312
                     /locus_tag="VFG22_09715"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET6416562.1"
                     /translation="MRHSLGRRVTSLLVAGLTLGWGCAHVPKRFPLPEEHVESAEVLG
                     IARARLWGDAPPPWEHDWLAKNKTDMKDRYPGIYGRRHAYLAISGGGEDGAFAAGLLL
                     GWTEAGDRPEFTTVTGISAGALIAPFAFLGPEYDDVLRKVSVDVTEKDLFNKRGKIRT
                     LRTDALATTEPLQALIAKYVNDETMQKIAAEHRKGRELNIGTTNLDSMRPVVWRIGVI
                     ANSGHPDALKLIRQILLASASVPGGFPPVLIEVQAGNKEYDELHVDGGATSQVFIYPV
                     GIDYDEILDKLSVTGRPEVYIIRNARLDPTYEAVKNRFLPILGRSIESIVRTQGIGDL
                     YRIYLQTCRDGLDFNLAYIPSDFTAESEGYVDTEYMKKLFDTAFDLAKAGYAWDKAPP
                     QLDTESIRCN"
     gene            complement(1315..2445)
                     /locus_tag="VFG22_09720"
     CDS             complement(1315..2445)
                     /locus_tag="VFG22_09720"
                     /inference="COORDINATES: protein motif:HMM:NF019436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416563.1"
                     /translation="MSSPTHHIVLIPGMFGFSRLAGYDYFVHIATALGDRMRTAGESC
                     ELVVVSAPPTGSIRRRTQVVIDTIEEHCTKGEGPIHVIGHSTGGLDARLLTSPSVTLD
                     VPSWIERVRTVVTLNTPHRGTPVAYFFSTLSGTRLLYALSLFTFATLRFGGPSLTVFS
                     SWIAFLGRLDNAFGVNIKLLDRSVQLVLRFIEKEGQIEVGEWLDGIRHDQGGVIQLTP
                     ESMDMFNASVEDSSKVRYGSVASWGPSPGPVRFLSSARSPMAALSATLYSTLHVIASR
                     ASKTYPYSSVEGTAREMIEHALGRELTDEVNDGLVPTASMLWGDLLWAGAADHLDILG
                     HFHGEPGSTHTDWLTSGAGFREDDFGQVMDAVTDFLLDRRCI"
     gene            complement(2553..3623)
                     /locus_tag="VFG22_09725"
     CDS             complement(2553..3623)
                     /locus_tag="VFG22_09725"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HET6416564.1"
                     /translation="MDYLDSIVVLLRDWLAVLITILVGILVIWGAYRLLLKRQASTSK
                     SRLFQQVIMVMLVAIFVITLILELPIGDAPRGQLLSLLGILVAAAIALSSTTLLGNAM
                     AGLMLRSLRNFRPGDFIIVEGIRGRVSELSLLRTEIQTERRELTTLPNLYLVTNPVTV
                     VRSSGTLISATVSLGYEIPREKVEKHLLEAAENSGLSDPFTFVLQLGDFSITYQIAGF
                     LEEVKFLISAESKLRENMLDALHGAGIEIVSPTFMNQRQLSTSQVFIPETKLATKAEP
                     EAAEEPRPEERMFDKAELAETESKAEQELKNVIEELEALKKADAEASKPRISELEARV
                     AELEAAIKMRQEQKEESNDGED"
     gene            complement(3791..4909)
                     /locus_tag="VFG22_09730"
     CDS             complement(3791..4909)
                     /locus_tag="VFG22_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416565.1"
                     /translation="MSDVERNELSVAMAIADRIKELIAGLRRDRITELIRRHLLQFRL
                     IDGTGRWQAGELAALGDWLLGEDLDTDDALELVDEAAHCAAANPYLTEFLDRAKGQVF
                     DRLYQKVQMEGARVLPSVVTYGMLLERLTQAMRNDWGIIKACREIWMRARNADGMNSH
                     LTVFERAKVLSIDVTIDWVLGFHESGSVAPKFTRVLLPMNIIEAMFEDVRLGNRDNAA
                     SAWPLVKDRPGSWAREAKTGAVAAHWSEDRKAISLSFALPRKWADLYATWNLAFVSHY
                     GDFPYLMTKLLIPQVNGYQNCPEEYIYNRLLALYCQLHYTGFGRVDLARQGRDAIDWH
                     DEALTKLWSSVNRQSAAKYSEAVRQLERAGLRRSTFPG"
     gene            complement(4933..>5665)
                     /locus_tag="VFG22_09735"
     CDS             complement(4933..>5665)
                     /locus_tag="VFG22_09735"
                     /inference="COORDINATES: protein motif:HMM:NF024585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET6416566.1"
                     /translation="CRHKAEHLGKACDVPQEICLSLNGGANFVIGRGFGRPIDKQEAL
                     SIVKKARKLGLVQIADNVKNEPAYVCNCCACCCGQLSSINDFGLKAVNPSGFEPAHHP
                     DNCKGCSRCSRACPIAAITMAPARTGGQRKNELRPVFDSELCIGCGVCATACKQDAIS
                     MERRESPSHVPENTIERVVRQMLEHGRLADLLFDESESRGAAFLNHAVQAITKLPPAQ
                     RVLASDQVRSRFVQFVLKNAPAIEA"
BASE COUNT         1136 a         1772 c         1659 g         1098 t
ORIGIN      
        1 cccaagatgc actgcgaacg aaaagcgcgg tcaagccaag cgcacgtgaa gagaatgacc
       61 aacgcatgcc agtcgattgc tattcgtgct tggagcattc ctgatgcgac atagccttgg
      121 gcgtcgcgtc acctcgctgc tcgtcgccgg gctcacgctc ggctggggct gcgctcacgt
      181 tccaaagcgt ttccctttac ccgaggagca cgtcgaaagc gccgaagtgc tcgggattgc
      241 gcgagcgcgg ctatggggtg acgcgccccc gccatgggaa cacgactggc tggccaagaa
      301 caagacggac atgaaggatc gctaccccgg catatacggt cgtcgtcacg cctatcttgc
      361 gatttctggg ggcggtgagg acggcgcgtt tgccgcaggg ctcttgctcg gttggacaga
      421 agccggggat cggccggagt tcacgacggt caccggcatc agtgcggggg cgctcattgc
      481 gcccttcgcg ttcctcggac ccgagtacga cgacgtgttg aggaaggtct cggtggacgt
      541 caccgagaag gatctattca acaagcgtgg gaagatcagg acacttcgca ccgatgccct
      601 agcgactacc gaaccgctcc aagcattgat cgcgaagtac gtgaacgacg agacgatgca
      661 gaagatcgcc gccgagcatc gcaagggccg ggagctgaac atcggcacca cgaacctcga
      721 ttcgatgcgg cctgtagttt ggcgcatcgg agtgatcgca aacagcggcc atcccgatgc
      781 cttgaagcta atccgccaga tcctgcttgc ctcggcgtcc gttccgggcg ggtttccacc
      841 cgtgctcatc gaggttcaag caggcaacaa agagtacgac gagctccacg tcgatggtgg
      901 cgcaacctcg caagtgttca tctacccggt cggcatcgac tacgacgaaa tcctcgacaa
      961 gctctctgtg accggccgcc cggaggtcta catcattcgc aacgcacgcc tcgatccgac
     1021 gtacgaggca gtcaagaaca ggttccttcc catcttgggc cgctcgatcg agtcgatcgt
     1081 ccgcacccaa ggcatcggcg atctctaccg catctacctc caaacgtgcc gcgacggtct
     1141 agacttcaat ctcgcgtaca ttccctcaga cttcacggcg gagtccgaag ggtacgtcga
     1201 cacggagtac atgaagaagc tcttcgacac ggccttcgac ctcgccaagg cgggctacgc
     1261 ttgggacaag gcaccgcccc agctcgacac cgagtcgatc cgctgcaact gaagtcaaat
     1321 gcaacgacgg tcgagcagaa aatcggtgac ggcatccatc acctgtccga agtcgtcctc
     1381 acggaagcct gcgccgctcg tcagccagtc ggtgtgggtc gagccgggct caccgtggaa
     1441 gtgaccaagg atgtcgagat ggtccgccgc tcccgcccaa aggagatctc cccagagcat
     1501 gcttgccgta ggcaccaagc cgtcattgac ctcgtctgtc aattctcgac cgagcgcatg
     1561 ctcgatcatc tcgcgcgccg tcccttcgac ggaagagtag ggataggttt tcgaagcgcg
     1621 actcgcgatc acgtggagcg tcgaatacag cgtggccgag agtgcggcca ttggcgaacg
     1681 agcactcgaa agaaaccgaa ctgggcccgg tgacggaccc cagcttgcga ccgagccata
     1741 tcgtaccttc gagctgtctt cgacggacgc gttgaacata tccatcgatt ccggagtgag
     1801 ttggatcacg cctccttggt cgtgacggat cccgtccagc cactcgccca cttcgatctg
     1861 cccttccttc tcgatgaatc gcaaaacgag ctgaacgctc ctgtcgagca gcttgatatt
     1921 caccccaaag gcattgtcga gcctgcccag gaatgcgatc cacgacgaga acacggtcag
     1981 cgaagggccg ccgaatcgca gcgtcgcaaa cgtgaaaagc gagagcgcat agagaagccg
     2041 tgttcccgac agggtagaga agaaataggc caccggcgtg ccccggtgag gggtgttgag
     2101 ggtgacgacg gtgcgaacgc gctcgatcca cgacggaacg tcgagggtga cgctcggcga
     2161 ggtcaacagc cgagcgtcga ggccgcccgt cgaatggccg atcacgtgaa tgggtccctc
     2221 acccttggtg cagtgttcct cgatcgtgtc gatcacaact tgggttcgtc ggcggatgga
     2281 ccccgtcggc ggcgcggata cgaccacgag ctcgcaagac tcgccggctg ttcgcatgcg
     2341 gtcccccaac gccgttgcga tgtggacaaa atagtcatag ccggccagac gtgaaaagcc
     2401 gaacatgcct ggaatcagga cgatgtgatg cgtaggcgag ctcatctggg ttcctgccag
     2461 cgaatactcc cgtgtttgtg cgtgtcaacc ggagggacgc gcccgagcgt ggcagtttgc
     2521 ctccacgcct cgacaacgcg gtcggctaat ccctaatcct ctccgtcgtt tgattcctct
     2581 ttctgttcct gacgcatctt gatcgcggcc tcgagctcgg cgacccttgc ctcgagctcg
     2641 ctgatacggg gtttggaggc ttctgcgtcg gcctttttga gcgcctcgag ctcctcgatg
     2701 acattcttga gctcctgctc cgccttagac tcggtttcgg cgagctcggc cttgtcgaac
     2761 atgcgttcct cgggtcgggg ctcctcagcc gcctcgggct cggctttcgt tgcgagcttc
     2821 gtttcaggga tgaacacttg actggtcgag agctgacgct gattcatgaa cgtcggtgag
     2881 acgatctcga tgcccgcacc gtgcaaggca tccaacatgt tctcgcgaag cttcgattcc
     2941 gcgctgatca ggaacttcac ttcctcgaga aaccccgcga tttggtaggt gatcgagaaa
     3001 tctccgagct ggagaacgaa tgtgaaaggg tcgcttagac ctgagttttc ggcggcctcg
     3061 agcaggtgct tttcgacttt ctcgcgcggg atctcgtatc cgagtgaaac cgtcgcggag
     3121 atcaacgtgc ccgacgagcg aaccaccgtc accgggttcg tcacgaggta tagattcggc
     3181 agcgtagtca gctcccgtcg ctcggtctgg atctccgtgc ggagcagtga gagctccgac
     3241 acacgcccgc ggattccctc gacgatgatg aagtccccgg gtctgaagtt tcgcagcgac
     3301 cgcaacatca gccccgccat ggcgtttccg agcagcgtcg tcgaagacaa agcaatcgct
     3361 gccgccacca gaatcccaag caggctcaaa agctgcccgc gtggcgcatc tccgatcggt
     3421 agctcgagga tcagcgtgat gacgaagatc gcaaccagca tgaccatgat gacctgttgg
     3481 aacaaccggc tcttcgacgt agaggcctgg cgtttgagca gcagacggta ggctccccag
     3541 atgaccagga tgccgaccaa gatcgtgatc agaacggcga gccaatcccg gagaagaaca
     3601 acaattgagt cgagatagtc catgagcatg cgcaagcgtc gcagaaatca tgccctgacg
     3661 taaaaatctc agcccaagcc gctcgggttt ccgcccgtcc atgaataagg cggcggctgc
     3721 agcacgcgcg acgagtgcta cccagatcca aagtctacgc ttccaatgcc cgatatctga
     3781 gcatctcact tcaacctgga aatgtggaac gacggagccc ggcgcgctcc agctgcctga
     3841 cggcttctga gtacttcgcg gcgctttgcc ggttgacgct gctccagagt ttcgtgagcg
     3901 cctcgtcgtg ccaatcgatc gcatcgcgac cctgccttgc aagatcgaca cgcccgaacc
     3961 ccgtgtagtg aagttggcag tacaaggcca agaggcggtt gtagatgtac tcctccgggc
     4021 aattttgata gccgttcacc tgaggaatga gcagcttcgt catcagatat gggaaatcac
     4081 cgtagtgaga gacaaacgcc agattccagg tcgcatacaa atccgcccat ttgcgcggca
     4141 gcgcgaagct cagcgaaatc gccttcctgt cctccgacca gtgcgccgct accgcgcccg
     4201 tcttcgcttc gcgagcccat gacccgggcc ggtctttcac gagcggccac gcgctcgctg
     4261 cgttgtcccg attgccgagc ctgacgtctt cgaacatcgc ctcgattatg ttcatgggca
     4321 gcagcactct cgtgaacttt ggagccacgc tacccgactc gtgaaacccg agaacccagt
     4381 cgatcgtgac gtcaatcgaa aggaccttgg cgcgctcgaa gactgtcaag tggctattca
     4441 taccgtccgc gtttcgggct ctcatccaaa tctctcggca ggctttgatg atcccccagt
     4501 cgttgcgcat cgcttgggtc aggcgttcga gcagcatccc atacgtcacc accgagggca
     4561 gaaccctcgc tccctccatt tgaaccttct ggtacaggcg atcgaaaacc tggcctttcg
     4621 cccgatccag aaactcggtc aggtacgggt tggccgcagc gcagtgagct gcttcgtcga
     4681 cgagctcgag cgcgtcgtcc gtgtccaggt cctcgcccag gagccagtcg ccgagtgccg
     4741 ccaactcccc cgcttgccac ctgcccgtgc cgtcgatcag gcgaaactgc aggagatgcc
     4801 gccggatcaa ttcggtgatg cgatcacggc gcagcccggc aatgagctcc ttgatgcggt
     4861 cggcaatcgc cattgccacc gagagttcgt ttcgctctac atcactcatg tccgctcctg
     4921 ctcaagggga gtctaggctt cgatggcggg cgcgttcttc aacacaaatt gaacgaaccg
     4981 cgacctcacc tgatcggaag cgagcacccg ctgggcggga ggcagcttgg tgatcgcttg
     5041 taccgcgtgg ttgaggaacg ccgcgccacg gctctcgctc tcgtcgaaga gaaggtcggc
     5101 caggcgaccg tgctcgagca tttggcgcac gacacgctcg atcgtgtttt cgggcacgtg
     5161 gcttgggctc tcccggcgct ccatgctgat cgcatcctgc ttgcaggccg tggcgcaaac
     5221 gccgcacccg atgcagagct ccgaatcgaa gaccgggcgc agctcgttct tgcgctgtcc
     5281 tccggtgcgc gccggtgcca tcgtgatcgc cgcgatggga cacgcccgag agcatcgcga
     5341 gcaacctttg cagttgtcgg ggtggtgcgc tggctcgaag ccgctcgggt tgacggcctt
     5401 gagcccgaag tcgttgatgc tcgaaagctg cccgcagcaa cacgcgcagc aattgcacac
     5461 ataggcaggc tcgttcttca cgttgtcggc gatttgaacc agcccgagct tccgcgcctt
     5521 tttcacgatg ctcagcgcct cttgcttgtc gatgggacgg ccgaagccac gcccaatgac
     5581 gaaatttgcg ccgccgttga gcgacagaca tatctcctgg ggaacgtcgc acgccttgcc
     5641 caaatgctcg gccttgtgcc ggcag
//
LOCUS       DASRZF010000219        12825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2901221, whole genome shotgun sequence.
ACCESSION   DASRZF010000219 DASRZF010000000
VERSION     DASRZF010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 12825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12825
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2901221"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="VFG22_09740"
     CDS             <1..551
                     /locus_tag="VFG22_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416567.1"
                     /translation="AQLVEDARWDLILVDLESTGHALMFFDLPSVLEVFLKDGPLRQV
                     LDSATKLVRDVDRSVIHIVTVPEPLAVNETILLYRRLLARDDLHLGHLFVNRYPPIWF
                     DEEEQRLLHAELNGPSRGQRWAADLELAEYLVRRRETADKCLRGLRRDIDLPTTVFDA
                     QDQGSAAIIEALSRAIELSEVW"
     gene            548..1627
                     /locus_tag="VFG22_09745"
     CDS             548..1627
                     /locus_tag="VFG22_09745"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HET6416568.1"
                     /translation="MIRLPEDCKLAFCVGVGGVGKTTFAAALALREALNGRSVLVLTA
                     DPARRLADALGMHALRDAPSRIALPTGAAGGQLHAAMLETKASADEIIRRAANDEDRA
                     QRVLDNRIYQAFSNTLARSHAYAAMERVYEAVQDPRYDLVVLDTPPAQSSIEILDAPA
                     RLVRFLDQRVVRWFLRAANDSQPLRRPPLSQRLLRAIAGDSLVSALSEFLTEMAFLRE
                     GFAARARVVRDLMRSTSTKFVLVSTADTVGIEAARSVAAELVGRDFELSALVCNRSFI
                     PELVSSGREATTYPARLAALAPKLERMRTLLTDEQERKHANMLALSGELGVAGWALPE
                     ASRSLAHTSALATWIEQARPVGPTP"
     gene            1645..1992
                     /locus_tag="VFG22_09750"
     CDS             1645..1992
                     /locus_tag="VFG22_09750"
                     /inference="COORDINATES: protein motif:HMM:NF021188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HET6416569.1"
                     /translation="MPSSVAPGGLMEHIIKHDLSPELAKKAAEKAAEHYTQKWQKYNA
                     KTTWVTDSKAEITFHVKGVHVAATVDMQPGQAVIEMDKVPLLLRPFKNMALDVVQKTM
                     QKWIDKAKSGELA"
     gene            complement(2070..3413)
                     /locus_tag="VFG22_09755"
     CDS             complement(2070..3413)
                     /locus_tag="VFG22_09755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6416570.1"
                     /translation="MPSNKSSDNTMMNEVIDAVKPRGWITYDEASQVIAKIGATQGTV
                     RKFMRRLEAAGVEVTVKQGKNRRGDRRRSAKLNENTNVLSAEPVQTYLDWMGAVSLLT
                     REGEVELARQIESGRQTIYDAVVAQGLHAEEIAEVRERVTTGELAVREAVIESSFESV
                     LAALGSYAQSCEEGSTARKKAEADSGLPASRLRQLHELVCTALAEVERAKSEMVEANL
                     RLVVAIAKKYLKRGLPFMDLIQEGNMGLMRAIDKFDYRRGYKLSTYASWWIRQSMARA
                     TTDQARTIRIPVHACELLTKVTGMTRKLTGELGREPTAAEIAKRMKLPADQVANLLMI
                     SRDTISLEMPVGNDGNSELRDLISDEEAATPMDSLVGEDLSKSVLRALDALNPREQRI
                     IRMRFGIGEKEAHTLAEIGREFGLSRERIRQLQALALRKLRNVHGDAALKQFVSA"
     gene            3589..5097
                     /locus_tag="VFG22_09760"
     CDS             3589..5097
                     /locus_tag="VFG22_09760"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET6416571.1"
                     /translation="MQGFSEYDAHDALGLAELVRRGEIKSGELVEACISRIRRLNPEI
                     NSVVFELFDAARERSKETLGGPFAGVPFLVKDLIQRIPGVPTQEGSRFFKGWTPSDET
                     NLYRRWIDAGVIPVAKTATSELGLLPVVETELHGATRNPWMLDRTAGGSSGGSGASVA
                     AGIAPMASGGDGGGSIRIPASCCGLFGHKPSRARNPSGPFASEHWSGYVSEHVLTRTV
                     RDSAAMLDATHGPEETSPYYAAPIEGTFLEAAERDPRPLKIAFHCEPALPATVHPDCK
                     AAVEDAARLCAELGHRVDEVSPGHDTMALGRAFLKVIGANVAAEIREGEKTRNRRATS
                     KDFQLTTWVTGMLGRAISGEDFLVAARELQTEARRLVDVYRDYDLILTPTLGTPPVRI
                     GELDPKGADLIAQKVLAALDWSAPLASTKIIDQAAQSTFSFVPFTPVANFTGQPSMSV
                     PLHWNAEGLPIGVMFTGRPEEETLMFSLAGQLERARPWRDRRPPLNARPINH"
     gene            5181..6797
                     /locus_tag="VFG22_09765"
     CDS             5181..6797
                     /locus_tag="VFG22_09765"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HET6416572.1"
                     /translation="MPEEQPYPVLRSRADTKSETYQSNRAGNLAALDAVNQALAKANA
                     GGGEKYVSRHLARGRILPRQRIEMLLDRDSFFLEIAALAGHGMKGEVSGSSVVGGVGL
                     VSGVECCIVASEATVKGGAVTPISMLKTGRLAEICEQNRLPGISCIESAGADLPKQDQ
                     IFVPGGAGFRHLTQRSENKIPTICLVFGSSTAGGAYMPGMSDYVVMVKEQAQVYLAGP
                     PLVKMATGEETDHEELGGAEMHSTISGVSDYLAEDEMDAIRLGREIVAHLEWEKRGRL
                     RPRPVEAPLHDPEELLGIASADIRLPFDQREVIARIVDGSRFSEFKPLYGKTMLCGWA
                     HIHGFPVGIIANNGTIFSDSANKGAQFIQLCNSSDVPLVFLQNTTGFMVGKQYEQNGI
                     IKHGAKMINAVSNSTVPAITIMTGSSYGAGNYAMMGRSYRPRFLFTWPNHKIAVMGGK
                     QLAGVLDIIQRDAAAKKGIAVDEKMLEVAKMMTEKQIEDQSTALYATARVWDDGIIDP
                     RDTRTVIAISLSAAYNASVEGTTSWGVFRH"
     gene            6815..8794
                     /locus_tag="VFG22_09770"
     CDS             6815..8794
                     /locus_tag="VFG22_09770"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HET6416573.1"
                     /translation="MTNTIQKILVANRGEIARRIMRTCRKMGIATVAVYSDADAEMPF
                     VAEADEAVCIGPAPSTESYLRIDKILEAAALTKSDAIHPGYGFLAENAAFADACAAGG
                     VIFIGPTPDAIRSMGSKREAKALVEKAGVPVIPGYDGASQDPAVLAAEAIRVGFPVLL
                     KASAGGGGKGMKLVREKAGLPDAIASAAREGQSSFGDGTLLVEKYIEDPRHVEIQILG
                     DRHGNLIHLNERECSIQRRHQKIIEETPSPALDASLRARMGEAAVLCGKAIGYQNAGT
                     VEFILAPDRSFYFLEVNTRLQVEHPITECVTGLDLVEEQILVAQGDALRLSQDEVRFD
                     GAAIEVRLYAEDPAAGFLPQSGRVVDWHLPSAAGLRVDSGVETGTEVGIHYDPMLAKI
                     ITSGESRQLALQRMRSALRSLSVQGVTTNREFLLQVLDHPAFVAGDIDTHFIDRHLQD
                     ALGKGVAEVDMQRAAMAAALAEQQQRETERLILRTIPSGWRNNYHTPQTVEYERGELA
                     VRVGYRHLGSNRFTVWTGEVARDVRVVSWESPTLVLEEGSHRWTARVLFDGDRTYVHA
                     ANFSLGLARKPRFPDKSLAIPAGGCVAPMPGKVIELRVAEGDAVTAGQVLLIMEAMKM
                     EHSVTAPRDGTVAQLMVVAGDQVDADALMIVVSEG"
     gene            complement(8804..9985)
                     /locus_tag="VFG22_09775"
     CDS             complement(8804..9985)
                     /locus_tag="VFG22_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET6416574.1"
                     /translation="MLSDKVLDNLPHEIRRRLEPYVEAVQLMTATNNLKVLRSMAPSG
                     VRGLILQRGKQGVPTTMPSHHRVYFDWTYPNDQPEMRELYTRAKEQQWNGDDLPWHID
                     VDPENPEKPIIPRQFMDYDALRGMGIKLTDLEERRLNASVASWMLSQFLHGEQGALFA
                     AAQVTEAVQFFDGKLYGSTQVVDEGRHVEVFHRYLDSKMNKLYQINDNLFVIIDALIQ
                     DSRWDVKFLGMQIMVEGLALGAFGTMYQMTGEPMLKQLLKMVIQDEARHVHYGVLALR
                     DHFRSELSESERREREDWAFEVALLMRNRFLSFEVYEEWFEHKLTRKAWAQFVTDSPG
                     FAIFRQVMFKRLVPNLREIGLLSDRIRPHYERAGLMKYADGESATQLSGDQMIAELDA
                     A"
     gene            complement(10000..10743)
                     /locus_tag="VFG22_09780"
     CDS             complement(10000..10743)
                     /locus_tag="VFG22_09780"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET6416575.1"
                     /translation="MTASAEALNLDEERWCYRMKDLCEATSLPRQAIHFYIQQGLLPP
                     GRKTGRNMAWYTEEHVERLHLIKKLQHERFLPLKAIKALFDGEDGVFSPRQHAFLAGV
                     KERLAESLKTSEDRKPTLTPREIAESTGVDAEDVERAFELGLVGGAESNGSLRIAAAD
                     VWIFEVFGEIRSFGFTPDLGFRVEDIALYQDAITNLLNEEVRLVSSRLSGLPPEKVAM
                     MIEAVVPILDRYIMRLHSTKIREFFSEVL"
     gene            complement(10740..12152)
                     /locus_tag="VFG22_09785"
     CDS             complement(10740..12152)
                     /locus_tag="VFG22_09785"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HET6416576.1"
                     /translation="MEGNRRRRPGRLDAAASLAARALPNAFTPWIDQARLDARMVIDR
                     TSGRSPAPLLDAIETPVPLGEVDPLDLLPRAIADRVRAVGRDLGMLGKRLVLGQTPPP
                     LVDRPRAGSGAIVSLPRKTPNMARPVKVVKVVRETKDAVSIYLTEADGSPIEFRPGQF
                     LSVDVNVDGQRLRRAYSLASACLPGVPVHVTVKRIEGGRASNQLNETIKEGDQLEVLG
                     PSGNFTVEPRSVNERHLVMVAGGSGITPIMSIAETVLRVEAASRVTLIYGNRSLDDVI
                     FRDRLASLCEELGGRLHVDHVLERPPNDWQHGRGLLTGEVLESRLQALGIEDDGVTRY
                     FLCGPTPMMEAAHEALQRRGVDASRIAEERFTSPEARSGNAGSYKTELVVVAKAGHDH
                     GIQVEPGQTILEAALAAGIEMPFSCAMGGCGACRVRRAEGEVQMEEPNCLSRAERERG
                     YVLTCVGRPLSQCKIEVEGS"
     gene            12239..>12825
                     /locus_tag="VFG22_09790"
     CDS             12239..>12825
                     /locus_tag="VFG22_09790"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET6416577.1"
                     /translation="MAPSPLISAAEVLPLIGRAVFLDARAGPDADRAYAGAHLSGAIH
                     LDAESALAVPGDPSDGGRHPLPALDAWLERLGACGVRPGTPVIVYDSAGGGMAAARAW
                     WMLRAVGHEPLAVIDGGWQAVVDAGLPIESGIAIGSRSEKYPSAIERWPTVDANWVER
                     VRQDPTWRLIDARAPERYQGSSEPLDPVAGHIPGA"
BASE COUNT         2399 a         4002 c         3965 g         2459 t
ORIGIN      
        1 tggcgcagct cgtcgaagac gcccgctggg acctgattct cgtcgacctc gagtcgacgg
       61 gacacgcact gatgttcttc gatcttccca gcgttctcga ggtcttcctc aaggatgggc
      121 cccttcgcca ggtcttggac agcgccacga agctcgttcg agatgtcgat cgctcggtga
      181 ttcacatcgt gacggtgccc gaacctctgg ccgtcaacga aacgatcctc ttgtaccggc
      241 gtctgctagc acgagacgac ctgcacctgg gccacctctt cgtcaaccgc tacccgccga
      301 tctggttcga cgaggaggaa caacgactgc tccatgccga gttgaacggt ccgagccggg
      361 ggcagcgctg ggccgcggat ctggagcttg cggaatacct ggtgcggcga agggagacgg
      421 ccgacaagtg cctacgcggg cttcgccgcg acatcgactt acccacgacc gtcttcgacg
      481 cccaggatca gggaagcgcc gcgatcatcg aagcgctctc gagagcgatc gagctgtcgg
      541 aggtctggtg atcaggctac ccgaggattg caagctcgcg ttctgcgtgg gtgtcggtgg
      601 ggttggcaag acgacttttg cggccgcatt ggcactgcga gaggctctaa acggcaggtc
      661 ggtgctcgtc ttgacggccg atccagcgcg gcgcctcgcg gacgcgctgg gaatgcatgc
      721 gttgcgtgat gctccctcgc ggattgcgct gccaacaggc gccgccggtg ggcagttgca
      781 cgctgccatg ctggagacca aggctagcgc ggacgagatc atccgcaggg cggcgaatga
      841 cgaggaccgg gcgcagagag ttctcgacaa tcgcatctac caggccttct cgaatactct
      901 cgcgcggtcg cacgcttatg cggccatgga gcgggtctac gaggcggtgc aggaccctcg
      961 ttacgacctc gtcgttctcg atacgcctcc cgcgcagagc tccatcgaga tcctcgatgc
     1021 gccggcccgc ttggtacggt ttctcgacca gcgcgtagtt cgttggttcc tccgagccgc
     1081 caacgattcc cagccgctcc gcagacctcc gctctcccaa aggctcctgc gtgcaattgc
     1141 aggggactca ctcgtctcgg ccctgagtga attcctgacc gagatggcat ttcttcgcga
     1201 gggtttcgcc gctcgcgcga gagtggtgcg cgacctcatg cgatcgacct cgacgaaatt
     1261 cgtactcgtc tcgactgcgg atacggtggg catcgaggct gcgcgatcgg ttgcagccga
     1321 gctcgtgggc cgcgatttcg agctctccgc gctcgtctgc aaccgctcat tcattcccga
     1381 gctcgtctcc agtggccgcg aagcgacgac gtatccggct cgactggccg cgctggcgcc
     1441 aaagctcgaa cgaatgcgga cgcttctcac cgacgagcag gagcggaaac acgccaacat
     1501 gctcgcactc tccggtgagt tgggagttgc cggctgggcg ctccccgagg cgtctcgttc
     1561 gctcgcgcat acgtccgcgc ttgcgacctg gatcgagcaa gcacggccgg tcggtcccac
     1621 gccgtagcga caactcgcat tttcatgcca tcctccgtcg caccgggagg cctcatggag
     1681 cacatcatca agcacgacct cagtcccgag ttggcaaaga aggctgctga gaaagccgcg
     1741 gagcactaca cgcagaagtg gcagaagtac aacgccaaga ccacctgggt cacggattca
     1801 aaagcagaga ttacgtttca cgtgaagggt gttcacgtgg ctgccacggt ggacatgcag
     1861 ccaggccaag ccgtcatcga aatggataag gtcccgttgc tgctccggcc cttcaagaac
     1921 atggccctcg acgtggtgca gaagacgatg caaaagtgga tcgacaaggc gaagagcggc
     1981 gagctcgcat gagacgactc gcctagcgaa cacgtcgcct ccgttcgttt tcacccgcga
     2041 tgcggttcgg gaaccgcggc gctcatggct tatgcgctca cgaactgttt caacgctgcg
     2101 tcgccgtgaa cattgcgaag cttgcgcagc gctagcgctt ggagctgacg aatgcgctcg
     2161 cggctaagcc cgaactcgcg accgatctcg gccaacgtgt gcgcctcttt ttcaccgatg
     2221 ccaaatcgca tccgaatgat tcgctgctcg cgtggattca gcgcatcgag agcgcgaagc
     2281 acggacttcg agagatcctc accaaccagc gaatccatcg gggtcgcggc ctcttcgtct
     2341 gagatcaggt ccctgagctc gctgttgccg tcgtttccga ctggcatctc caaagaaatc
     2401 gtgtcgcgcg agatcatgag cagattggcc acctgatcgg ctggaagctt cattcgcttt
     2461 gcgatctccg ccgcggtcgg ctcacggcct agctccccag tgagctttcg cgtcatgccc
     2521 gtcactttgg tgagcaactc acatgcgtga accggaatgc gaatcgtgcg agcttggtcg
     2581 gtcgtcgcgc gggccatcga ctggcgaatc caccaggacg cgtacgtcga gagcttgtac
     2641 ccgcggcgat agtcgaactt gtcgatagcc cgcatcaagc ccatgttgcc ttcctggatc
     2701 aggtccatga acggcagacc tcgcttgaga tatttcttcg cgatggcgac gaccaaacgt
     2761 aggttggcct cgaccatctc cgatttcgcg cgctccacct cggcaagggc cgtgcagacc
     2821 agctcatgga gctggcgaag cctcgacgcg ggaaggccag agtcggcctc ggccttcttt
     2881 cgagctgtcg agccctcctc acacgattgc gcgtaggatc cgagcgcagc gaggaccgat
     2941 tcgaagctcg actcgatgac cgcttcacga acagcgagct cgccggtagt cacccgctcg
     3001 cgaacttcag cgatctcctc cgcatggagt ccctgcgcga cgaccgcgtc gtagatcgtc
     3061 tgacggccac tttcgatttg gcgtgcgagc tcgacttcgc cctcgcgtgt gagaaggctc
     3121 accgcgccca tccaatcgag ataggtctgc accggctcag cactgaggac gttcgtgttc
     3181 tcgttcagct ttgctgaacg ccgtcgatcg cctctgcggt tcttgccttg cttgacggtc
     3241 acctcgactc ctgcggcttc gaggcgccgc atgaacttac gcacagtgcc ctgggtcgcc
     3301 ccaatcttgg cgatcacctg tgaagcctcg tcgtacgtga tccaccccct cggcttcacc
     3361 gcatcgatca cttcgttcat cattgtgttg tcgctggatt tattcgatgg cattgctggt
     3421 tctccgctgt gtctaagcgt gtggtaccac cacgttggaa aattagcaac catatgatca
     3481 aaaaaatgta gttatactgt gaatgtaaca tcactgttgg ctagctcaca gtcctggatc
     3541 cggaaaaccc cattgctatt gagcctttgc ctgagttagg cctcggcgat gcagggattc
     3601 tccgaatacg acgcccacga cgccctgggc cttgctgagc tggtccggcg cggtgagatc
     3661 aaaagcggcg agctcgtgga ggcctgcatt tcacgcattc ggcgcctcaa tccagagatc
     3721 aactccgtag tgttcgagct cttcgacgcg gcccgggagc ggtccaagga gaccctgggc
     3781 ggccctttcg cgggcgtccc cttcctcgtc aaggacctga ttcaacgtat ccccggtgtc
     3841 cctacccaag agggctcgcg gttcttcaag ggctggaccc caagcgacga aaccaatctc
     3901 tatcggcgct ggatcgatgc cggagtgatc ccggttgcca agaccgcgac ctccgagctc
     3961 gggcttcttc ccgtggtcga aaccgagctt cacggggcca ctcgcaaccc ctggatgctc
     4021 gaccgaactg caggtgggtc gagcggcggt tcaggagcca gcgtcgcggc aggcattgcg
     4081 ccgatggcct ccggcggaga cggtggcggc tcgattcgca tacctgcttc ctgctgtggc
     4141 ttgttcgggc acaagccgtc cagggcgcgt aacccatcgg gccctttcgc gagcgagcac
     4201 tggtctggct atgtttccga gcacgtgctc acacgcaccg tccgagacag cgcggcgatg
     4261 ctcgacgcga cgcacggccc cgaggaaacg tctccgtatt acgccgcccc gatcgaaggt
     4321 acgtttctcg aggccgcaga acgcgatccc aggccattga agattgcgtt tcattgcgag
     4381 cccgcgctgc cggcgaccgt ccacccggat tgcaaagcag cggtcgaaga tgcggcgcgg
     4441 ctctgtgccg agcttggcca tcgggtggat gaggtatctc cgggacatga cacgatggct
     4501 ctcggccgcg cgtttctcaa agtgatcggg gccaacgtcg ctgccgaaat ccgcgaaggt
     4561 gaaaagacca gaaatcggcg cgcgacttcg aaagacttcc agctcacgac ctgggtcaca
     4621 ggcatgctcg gcagagcgat tagcggcgag gacttcctcg tcgccgcccg agagttgcaa
     4681 accgaggcgc gacggctggt ggatgtctat cgagactatg atctgatcct gactccgacc
     4741 ttgggaacgc cacccgttcg aatcggggag ctcgatccca agggtgctga cctcattgcg
     4801 cagaaggtgc tggctgcgct cgattggagc gctcctctgg ctagcaccaa gatcatcgat
     4861 caggctgcgc agagcacctt ctcgttcgtc cccttcacgc cggtcgcgaa cttcacgggc
     4921 cagccttcga tgtccgtgcc cctgcactgg aacgccgagg ggcttcccat tggagtgatg
     4981 ttcaccgggc gtcccgaaga agaaacgctc atgttctcgc tcgcggggca gctcgagcgc
     5041 gcgcggcctt ggcgcgaccg gcgtcctcct ttgaatgcca gacccataaa tcattagggt
     5101 ttcggggtcc ttccactgat tgaaccctca ttcatttttg ggttgcccga gcttgggccg
     5161 agcctctagg attcacccgc atgcccgaag aacagcctta cccagtcctg cgttcgaggg
     5221 ccgacacgaa gtccgagact taccagtcca atcgtgcggg caatctagct gctctcgacg
     5281 cggtgaatca ggcactcgcc aaggccaacg ccggtggagg cgagaagtat gtgagtcggc
     5341 atcttgcgcg cggccgcatt ctgccgcgtc agcggatcga gatgctcctc gatcgagaca
     5401 gcttctttct cgagatcgct gcgcttgcgg gtcacgggat gaagggagag gtcagtggtt
     5461 cgtcggtcgt cggcggcgtc ggcctcgtga gcggggtgga gtgctgcatc gtggcgagcg
     5521 aagcgaccgt gaagggtgga gcggtcactc cgataagcat gctgaagaca ggccgcttgg
     5581 ctgagatctg cgagcagaat cgcttgccgg gaatcagctg catcgagtcc gctggcgccg
     5641 atctcccaaa acaggatcag attttcgttc caggcggtgc gggcttccga cacctcacgc
     5701 agcgctccga gaacaagata ccaaccatct gcctcgtttt cggaagctcg accgcgggcg
     5761 gtgcatacat gcccgggatg agcgactacg tcgtgatggt gaaagagcag gcgcaggtct
     5821 accttgccgg tcccccgctg gtgaagatgg cgaccggaga agagaccgac cacgaagagc
     5881 tcggtggtgc cgagatgcac tcgacgataa gcggtgtttc cgactacctc gccgaagacg
     5941 agatggatgc cattaggctg gggcgcgaaa tcgttgcgca tctagagtgg gaaaagcgtg
     6001 gcaggcttcg gccgcgaccg gtcgaagcgc cgcttcacga cccggaggag ttgttgggaa
     6061 tcgcgtctgc ggacatccgg cttcctttcg atcagcgtga ggtgatcgcc cgaatcgtcg
     6121 atggatcccg cttcagtgaa ttcaagccac tctacggaaa gaccatgctc tgcggctggg
     6181 cccacatcca cggctttccg gtcggaatca tcgcgaacaa cgggacgatt ttcagcgaca
     6241 gcgcaaacaa aggcgcacag ttcatccagc tttgcaacag cagcgacgtt ccgctggttt
     6301 tcctgcagaa caccaccgga ttcatggtgg gtaagcagta cgagcagaac gggatcatca
     6361 agcacggcgc caagatgatc aacgccgtat cgaacagcac ggtaccggcg atcaccatca
     6421 tgactggatc gagctacggg gccgggaact acgcgatgat gggccgctcg taccgaccac
     6481 gcttcctctt cacctggccg aatcacaaga tcgcggtcat gggcggcaag caactcgctg
     6541 gcgtgctcga catcattcag cgagacgcgg ctgccaagaa gggcatcgca gtcgacgaga
     6601 agatgctgga ggtcgccaaa atgatgaccg agaagcagat tgaggatcag tccaccgcgc
     6661 tctacgcgac cgcgcgcgtg tgggatgacg gcatcatcga tcctcgggac acgcgtacgg
     6721 tcatcgcgat cagcctgtcg gctgcgtaca acgcttcagt ggagggcacc acatcatggg
     6781 gcgtgtttcg ccactaatag ggagcaagcg ggcgttgacc aatacgattc aaaagatcct
     6841 cgtggccaac cgtggcgaaa tcgcccgtcg gatcatgcgt acctgccgca agatgggcat
     6901 cgccacggtg gcggtctact ccgatgccga cgccgagatg ccattcgtcg ccgaggctga
     6961 cgaggcggtt tgcataggtc cggcaccgag caccgagagc tatctgcgca tcgacaagat
     7021 tctcgaagcc gctgctctca cgaagagcga tgcgattcat ccaggctacg gcttcctagc
     7081 tgagaacgcg gctttcgcag acgcctgcgc cgctggcggc gtgatcttca tcgggcccac
     7141 ccccgatgcg attcgatcga tgggctcgaa gcgcgaagcg aaggcgctcg tcgagaaggc
     7201 cggcgtgccc gtgattcctg gctacgacgg cgccagccag gatccagccg tgctcgcagc
     7261 cgaggccatc cgagttggct tccccgttct gctcaaggcc agcgcgggcg ggggcggcaa
     7321 gggcatgaag ctcgttcgcg aaaaggcggg gctcccagac gcgatcgcgt cggccgcacg
     7381 cgaggggcag agctcgttcg gggatggaac gctcctggtc gagaaataca tcgaagatcc
     7441 gcgccacgtg gagatccaga ttctgggcga ccgccacggc aatctgattc atctcaacga
     7501 gcgcgagtgc tcgattcagc ggcgtcacca gaagatcatc gaagagacgc cttcgcctgc
     7561 gctcgatgct tcgctgcgcg cgcgcatggg cgaggccgcc gtgctctgcg ggaaagcgat
     7621 cggctaccag aacgccggca ccgtcgaatt cattctggcg cccgatcgga gcttctactt
     7681 cctcgaggtg aatacgcgcc ttcaggtgga acaccccatc acggaatgcg tgacggggct
     7741 cgatctcgtg gaagaacaga tcctggtcgc ccagggcgac gcgcttcggc tctcacagga
     7801 cgaggttcgg ttcgacggcg cggcgatcga ggtgcgcctc tatgccgagg acccggccgc
     7861 cggctttctt ccgcagagcg gacgagtcgt cgactggcac cttccgtctg ccgcaggcct
     7921 gcgggtcgat agcggcgtcg agacggggac tgaggttggg atccactacg atcccatgct
     7981 ggccaaaatc atcacctccg gcgagagccg gcagctggcg cttcagcgca tgcgaagcgc
     8041 gcttcgatcg ctcagcgtgc aaggggtgac gaccaaccgg gagttcctgc ttcaggtgct
     8101 cgatcatccg gcattcgttg cgggcgacat cgatacgcat ttcatcgatc ggcacctcca
     8161 agatgcgcta ggcaaaggtg tcgccgaggt agacatgcaa cgcgccgcga tggcggcggc
     8221 tctcgccgag cagcagcagc gcgagactga gcggctgatc ctacggacga tcccttctgg
     8281 atggcgcaac aactaccaca ccccgcaaac cgtcgaatac gaacggggcg agctcgcggt
     8341 gcgcgtcggc tatcgtcacc tcggaagcaa tcgcttcacc gtctggactg gcgaagtcgc
     8401 gcgagacgtc agagtcgtgt cctgggagtc gcccactcta gtcctcgaag aaggatcgca
     8461 ccgatggact gcgcgtgttc tcttcgatgg tgatcggacc tatgtgcacg ccgccaattt
     8521 cagtctcggc ctcgcgcgaa agccgcgctt tcccgacaag tcgctggcta ttccagcagg
     8581 tggctgcgtg gcccccatgc ctggcaaggt gatcgagctc cgcgttgcag aaggcgatgc
     8641 cgtcacagca ggtcaggtgc tcctcatcat ggaagccatg aagatggagc actcggtgac
     8701 cgctccccgg gacggaaccg tggctcagct catggtcgtg gccggggacc aagtcgatgc
     8761 cgacgctctg atgatcgtag tctccgaagg ctgagcggcg cgctcaggcg gcgtctagct
     8821 cggcgatcat ctgatctccg ctgagctggg tggcagattc gccatcggcg tacttcatca
     8881 agccagcgcg ctcgtagtgc ggcctgatgc ggtcgctcag aagaccgatc tcgcggaggt
     8941 tcggcacgag gcgcttgaac atcacttgcc ggaagatcgc gaaaccaggg gaatcggtga
     9001 cgaactgggc ccaggccttg cgcgtcagct tgtgctcgaa ccactcctcg taaacctcga
     9061 acgagaggaa gcggttgcgc atcagaaggg cgacctcgaa ggcccagtct tcacgttcac
     9121 gcctttcgct ttcactcaac tccgatcgga aatggtcgcg gagcgcgagg acgccgtagt
     9181 gcacgtgacg agcctcgtct tggatgacca tcttcagaag ttgcttgagc atcggctccc
     9241 cggtcatctg gtacatcgtt ccaaatgcgc caagtgccaa gccttcgacc atgatctgca
     9301 tgccgaggaa cttcacgtcc cagcgcgagt cctggatgag agcgtcgatg atcacgaaca
     9361 ggttgtcgtt gatctgatag agcttattca tcttggaatc gaggtaacga tggaacacct
     9421 cgacgtggcg gccttcgtcc acgacttggg tcgagccgta gagcttgcca tcgaagaatt
     9481 gaacggcttc ggtgacttgg gccgcggcaa agagcgcgcc ttgctcgccg tgcaggaact
     9541 ggctcagcat ccacgacgcg acgctggcgt tgaggcggcg ctcttccaga tccgtgagct
     9601 tgatccccat gccgcgcagc gcgtcatagt ccatgaactg gcggggtatg atcggtttct
     9661 ctggattttc cggatcgacg tcgatgtgcc aggggaggtc gtcaccgttc cactgctgtt
     9721 ccttcgcgcg cgtgtagagc tcgcgcatct ccggttggtc gttgggataa gtccagtcga
     9781 agtaaaccct gtgatgcgat ggcatcgtag tgggcacgcc ctgcttgccc cgctgcagaa
     9841 tgagaccgcg cacacctgac ggtgccatcg aacgcagcac cttgaggttg ttcgtcgccg
     9901 tcatcaactg cacggcttct acgtagggtt cgagccggcg gcgaatctcg tggggcaagt
     9961 tgtcgagcac tttgtcactg agcatggcca gtcccttcct tagagcactt cggagaagaa
    10021 ttcgcgaatc ttggtggaat gtaaacgcat gatgtaacga tcgaggatcg gaacgacggc
    10081 ctcgatcatc atagcgacct tttcgggtgg aagccccgat agccgtgaag acaccaggcg
    10141 aacctcttca ttgagcaagt tggtgatggc gtcttgatag agcgcgatgt cctcgacccg
    10201 aaagccgagg tccggcgtaa aaccaaagga ccggatttcc ccgaaaacct cgaagatcca
    10261 aacgtctgct gcagcgatcc gaagggagcc gttggactct gcaccgccga ctagaccgag
    10321 ctcgaaggct cgctcgacgt cttcggcgtc gacgcccgtc gactccgcga tctcgcgtgg
    10381 tgtgagggta ggttttcggt cttcgctggt cttgagcgac tccgccagcc gctccttcac
    10441 gccggcgaga aacgcgtgct ggcggggcga gaacacgccg tcctcgccat cgaaaagcgc
    10501 cttgatcgct ttcaggggca ggaagcgctc gtgctggagt ttcttgatca gatgaagccg
    10561 ctcgacgtgc tcctccgtgt accaggccat gttgcggccg gtcttccgtc cgggaggaag
    10621 gagtccttgc tggatgtaga agtggatcgc ctggcgcggc aagctcgtgg cctcgcaaag
    10681 atccttcatg cggtagcacc aacgttcctc atcgaggttc aacgcttcag cgctcgccgt
    10741 catgagccct ccacttcgat cttgcattgc gagagaggac gcccgacgca cgtgagcacg
    10801 tagcctcgct cgcgttcggc cctgctcaga caattcggct cttccatctg aacctcgccc
    10861 tcggccctac ggacgcggca cgcgccacag ccacccatcg cacaggaaaa gggcatctcg
    10921 attccggcag ccagcgccgc ctcgaggatc gtctgtccgg gctccacttg gataccgtgg
    10981 tcgtgacctg cctttgcgac gacgacgagc tcggtcttgt agctccccgc gttgccagag
    11041 cgcgcctcgg ggctggtaaa ccgctcttca gcgattcggc tcgcgtccac cccgcgtctt
    11101 tgaagtgcct cgtgcgcggc ttccatcatc ggtgtagggc cgcaaagaaa ataccgcgtc
    11161 acgccgtcgt cttcgatccc gagcgcttgc agccgtgatt cgagcacctc gcccgtcaga
    11221 agacctcgcc cgtgttgcca gtcattcggt ggccgctcga gcacgtgatc cacgtgcaaa
    11281 cgcccgccaa gctcctcgca gagcgatgcc aatcgatcgc gaaaaatcac gtcgtccaag
    11341 ctgcggttcc cgtagatcag cgtcacgcgg ctcgctgctt ccacgcgaag caccgtttcc
    11401 gcgatgctca tgatcggcgt gatgccgctg cctcccgcga ccattaccaa gtgccgctcg
    11461 tttaccgagc gcggctcgac cgtgaagttg ccggaggggc ctaggacctc aagctggtct
    11521 ccttccttga tggtctcatt cagttgattg ctcgcacggc cgccttcgat tcgcttgacc
    11581 gtcacgtgaa cggggacccc tggcagacac gcgctcgcca aggagtaagc gcgtcgcagg
    11641 cgctgcccat cgacgttgac gtccacactg aggaactgcc ccggccggaa ttcgatgggt
    11701 gagccatccg cttcggtgag atagatcgaa acggcgtcct tcgtttcgcg aaccaccttc
    11761 acgaccttga cgggccgcgc catgttgggt gtcttgcggg ggagcgaaac gatcgccccc
    11821 gaaccggcgc gcggtcggtc gaccaaagga ggaggcgtct ggcccaagac caatcgcttg
    11881 cccagcatcc cgaggtcgcg gccgacggct cgcactctgt cggcgatcgc gcgaggaagc
    11941 aggtcgagcg gatcgacctc gccgagggga accggggtct cgatcgcatc gagaagcggc
    12001 gcaggggatc ggccgctcgt tcggtcgatc accatcctgg cgtcgagacg tgcctgatcg
    12061 atccacggcg tgaacgcgtt cgggagcgcc cgagcagcga gcgatgcggc tgcgtccaag
    12121 cggccgggac ggcggcggcg gttaccctcc ataagacgag cagagtgccg aacttcgaac
    12181 aaagcgtcaa gtgtgactag acactttact cacattgtgc gctagagtag gccatcgcat
    12241 ggctccgagt cccctcattt cggccgccga ggtgctccct ttgatcggac gagcggtctt
    12301 tctcgacgcg cgggccggcc cggacgcgga tcgtgcctat gccggcgctc atctcagcgg
    12361 cgcgatccat ctcgatgccg aatccgcgct tgcggtgccc ggcgatccat ccgatggagg
    12421 gcgccacccg ctgcctgctc tcgatgcttg gctcgagcgg cttggcgcct gcggtgtgcg
    12481 acctgggacc ccagtgatcg tatacgacag cgcgggaggc gggatggctg cggcccgtgc
    12541 ctggtggatg cttcgcgcgg tcggtcacga gcctctcgcg gtcatcgacg gcggatggca
    12601 ggccgttgta gacgcgggac tgccgatcga gtcaggcatt gcgatcggga gtcgatctga
    12661 gaagtacccc tcggcgatcg agcgctggcc gacggtcgac gccaactggg tcgagcgggt
    12721 gcggcaggat ccgacttggc gcctgatcga cgcgcgcgct cccgaacgat accagggaag
    12781 cagcgagcct ctcgatccgg tcgccggaca catccctggt gccca
//
LOCUS       DASRZF010000220         3838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2556177, whole genome shotgun sequence.
ACCESSION   DASRZF010000220 DASRZF010000000
VERSION     DASRZF010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2556177"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2226)
                     /locus_tag="VFG22_09795"
     CDS             complement(<1..2226)
                     /locus_tag="VFG22_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416578.1"
                     /translation="MKRTTRTPSGWIVLFLLGIAACGGESAAPREVTLEASSGPAASM
                     LEVYGLDFASCPLRTSEIRVGGETAPAVVGASGEVVMRLPLFYDEAMKWAAPPSEPQD
                     VEVFCNGRLWTMLPEALTVTELPPAPGTTQTIVADYAQISADYRRMLDVLVPEPGVQQ
                     QLFAAFFSALDEALTGTDPSSLPVLLGDLSRDDPEGLALMDAVHAASGVDQDVAAFKD
                     FIEEMSAQAVASAGAAPAGTPAGPAVSAPKAAEYDFPIPIIKSDIELAKAMQSYVVLD
                     DFSQQVIKQTSEKFSTGMGLLSVALRKVPASKAVSIFLLLADYVMNKLVVSALPANLD
                     LIDLAIPQTKLQNSEVTHSSFILYASNVPAKFSISDMTSIVLTVLGIADKEPATPGVA
                     ADWINSLEELLPQATMFLLERLHKAFEEYALVFPEGIKYDRELFSVVPKLHFQAFGET
                     RELYVLHPEHSEAVAPLADALEWQASPTHWGSAEVYITPGPGPEGGFWGSLYTGRAFG
                     VSDIRSNGVVVTVGDLSILIDQPTVSVPEGGSAAFGVKLSNPPADAQAAVVVNVEGKG
                     GDQDISIISGQSISFNESNWNAFQPVMLAAAEDEDDEDGQATISASALVDFGDEEPRK
                     VIASIEANEVDNDRLKFVSTEASVKVPEGETATFGLKLNQSPPSAVKARVRHSSGDPD
                     LFVEYPSNLRFNSSNWNTFQTVTLYALTDEDSVEGSAQIRIAADPPARVDDLYIRATE
                     DE"
     gene            2491..3777
                     /locus_tag="VFG22_09800"
     CDS             2491..3777
                     /locus_tag="VFG22_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HET6416579.1"
                     /translation="MLQRSSLVLLALSLTYCSSSTPEEAGEAGPGKLVESSKARITTP
                     DASSQEVGQLARDNAAFAWDFYREAAKPGENLFLSPHSISVALAMTWAGARRETEAEM
                     ADAMHFTLGQERLHAAFNALDLELQKRAEAGSAEAPLPFRLNVTNALFGQVGFSLLDP
                     FLDTLAQHYGAGMRLMDFVAETEQSRLAINQWVSERTEQRIEELIAPGIIDAATRLVL
                     VNAIYFTASWAEPFDEAATRDAAFRRLDGSEVMVPTMHQTVQAVYGEGEGFQATELPY
                     DGQQLAMLLLVPDEGKFAALEEALSERMVDEIRAGLSTHLVELAFPKFSFRSNVPAKA
                     PLQALGMVAAFGPGQADFSGMTDTAPLFIQDVVHQAFVAVDERGTEAAAATAVIVGET
                     SAPPPATLTVDRPFIFAIIDRTTGATLFLGRVLDPS"
BASE COUNT          687 a         1122 c         1272 g          757 t
ORIGIN      
        1 ctcgtcctct gtcgcccgga tgtacaggtc gtccaccctc gccggcgggt ccgctgcgat
       61 tctgatttgc gcggagcctt ccacgctgtc ttcgtcggtg agcgcgtaga gcgtgacggt
      121 ttgaaaggtg ttccagttgc tcgagttgaa ccgtaggttt gacggatact ctacaaagag
      181 gtcggggtcg ccactcgaat gccggactcg cgctttgaca gcggacggcg gggactggtt
      241 gagcttcagg ccgaaggtgg cggtctcgcc ctcgggcacc ttcacactcg cctcggtgct
      301 tacgaacttg agccggtcgt tgtcgacttc gttggcctcg atgcttgcga tgactttgcg
      361 gggctcttca tcgccgaagt cgacaagcgc cgaggcggag atcgtggctt gaccgtcctc
      421 gtcgtcttca tcctcggcgg cggcaagcat cacgggctga aaggcattcc agttggattc
      481 gttgaaggag atggactgcc cagagatgat ggaaatgtcc tggtcgccgc ccttcccctc
      541 gacgtttacg acgacggccg cttgcgcatc ggcgggtgga ttactgagtt tgacgccgaa
      601 ggctgcggac ccaccttcag ggacggagac tgtgggctgg tcgatcagga tggagagatc
      661 gccgaccgtg acgaccacgc cattggagcg gatgtcgctc acaccaaacg cgcggcccgt
      721 atagagtgag ccccagaacc caccctcggg cccaggtccc ggcgtgatgt aaacctcggc
      781 gctaccccag tgcgtggggc tcgcctgcca ctcgagagca tcggccaagg gcgcgacggc
      841 ctcgctgtgc tcggggtgca agacgtacag ctctcgggtc tcgccgaagg cttgaaagtg
      901 cagcttgggc acgacggaga agagctcgcg gtcgtacttg attccttctg gaaaaaccaa
      961 tgcgtactct tcgaaggctt tgtggagcct ctcgagcaaa aacatggtgg cttgcgggag
     1021 gagttcttcg agagagttga tccaatctgc agcgacgcca ggggtcgccg gctctttgtc
     1081 ggcgatgccg aggacagtca gcacgatgga cgtcatgtca ctgatagaga acttggcagg
     1141 cacgttgctg gcgtagagga tgaagctgga atgggtgacc tctgaattct gcagtttcgt
     1201 ttgggggatc gcgaggtcga tgaggtccaa gttcgcgggt agagccgaga cgaccagctt
     1261 gttcatgacg tagtcggcaa gcaacaagaa gatgcttacc gccttggacg ccgggacttt
     1321 gcggagggca acggacagca accccatacc ggtcgaaaac ttctcggacg tctgtttgat
     1381 gacctgctgc gagaaatcgt cgaggacgac gtaggactgc atcgctttgg ccagctcgat
     1441 gtcggatttg atgatcggga tcgggaagtc atattcggcg gctttgggtg cgctgacggc
     1501 tggccctgcc ggtgtacctg ctggagccgc tcctgccgaa gccaccgctt gggcgctcat
     1561 ctcttcgatg aagtctttga atgcggccac gtcctggtcg acgcccgacg ccgcgtgcac
     1621 agcatccatc agcgccaagc cttcggggtc gtctcgcgag aggtcaccga ggagcaccgg
     1681 caggctgctt ggatcggtgc ctgtgagcgc ctcgtcgagg gcgctgaaga aggccgcaaa
     1741 gagctgctgc tgaacccccg gctcgggaac caggacatcg agcatgcgtc gatagtccgc
     1801 gctgatctgt gcgtagtcgg cgacaatcgt ctgggtcgtg cctggtgcgg gcgggagctc
     1861 cgtcacggtg agcgcttcgg gcaacatggt ccagagacgc ccgttgcaga agacttcgac
     1921 gtcttgcggc tcgctcggag gggctgccca tttcatggct tcgtcgtaga acaggggcag
     1981 cctcatgacg acttccccgc tagcgccaac cacagcgggc gccgtctcgc ctccgactct
     2041 gatctcggat gtgcgtagcg gacaggatgc gaaatccaag ccgtagactt cgagcatcga
     2101 ggcggccggc cccgaagagg cttccagggt gacttcgcgc ggcgctgccg attcaccgcc
     2161 gcaagcggcg atgccgagga gaaatagaac aatccatcca gagggcgtgc gcgtggttcg
     2221 cttcatcgga ttaacgcgat gcatcaggcg tgccaggacg gcggccgggt tttcttcatg
     2281 gtcttgggcc agatcgcgca atcaatgcgc ttcgctcggc gtcctgcgca ttgggtgcgg
     2341 cggggcaggc gaagctctcg ccgaggggct ttgcgacggg cgcgagaagc ttcatcttgg
     2401 gaacgatgcg gagcttgtgc gcggcgacgc gattttcgca gcggtgcctg gcacgagacc
     2461 tgcgtgctac aagaaaacgg aggtagattc atgctacagc gttcgtcctt ggttcttctg
     2521 gctttgtcgc tgacgtactg ttcttcgagc acgcccgagg aagccggtga agcggggccc
     2581 ggtaagctcg tcgagtcatc caaggcccga atcacgactc cggatgcgag cagccaggag
     2641 gtcggccaac ttgcaaggga caacgccgcg tttgcctggg acttctaccg ggaagccgcg
     2701 aagcccggcg agaacttgtt cctgtcgccg cacagcatct cggtggcgct tgcgatgacc
     2761 tgggccggcg cgaggcgcga aaccgaagcc gagatggcgg atgcgatgca tttcacgctc
     2821 gggcaggagc gactgcacgc ggcattcaat gcgctcgacc tcgagttgca gaagcgggcc
     2881 gaagccggca gcgcggaggc gcccctgccg tttcgtctca acgtcaccaa tgctcttttt
     2941 ggtcaagttg ggttcagctt gctcgatccg ttcttggaca cgctggctca gcactacggc
     3001 gctggcatgc gtttgatgga cttcgtcgcc gagacggagc aatcgcggct cgccatcaat
     3061 cagtgggtga gtgagcgcac ggagcaacgc atcgaagagc tcatcgcgcc cgggatcatc
     3121 gatgcagcga ctcggctggt tctggtgaac gcgatttact tcacggcatc gtgggccgag
     3181 cccttcgacg aagcagcgac tcgggacgcg gcgtttcgac gcctcgatgg gagcgaggtg
     3241 atggtgccga cgatgcatca aaccgtccag gccgtgtacg gcgagggaga gggctttcag
     3301 gccacagagc tcccctatga cgggcagcag ctcgcgatgc tccttctcgt gcccgatgaa
     3361 ggaaagtttg cggcgcttga agaggcgctg tcggagcgca tggtcgacga gattcgagcg
     3421 gggctcagca cgcatctcgt cgagctcgcg tttccgaagt tctcgtttcg ctcgaacgtt
     3481 cccgcgaagg cgcctctaca agcgctcggg atggtcgcgg cgttcggtcc tggacaggct
     3541 gatttctctg ggatgaccga cacggcgccg ctcttcattc aggacgtggt gcaccaggcg
     3601 ttcgtcgccg tggacgaacg gggaaccgaa gccgcagcag ccacggcagt catcgtgggg
     3661 gaaacctccg cgcccccgcc tgcgacgctc acggtcgacc gtcccttcat cttcgcaatc
     3721 atcgaccgga cgacgggcgc gaccttgttc ctggggcggg tgctcgaccc ttcatgagct
     3781 tcggcgggtt cgtgctagag caggccctca ggccctgcgc atgcaaccga ttatttcc
//
LOCUS       DASRZF010000221        16937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3244895, whole genome shotgun sequence.
ACCESSION   DASRZF010000221 DASRZF010000000
VERSION     DASRZF010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 16937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16937
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3244895"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VFG22_09805"
     CDS             <1..240
                     /locus_tag="VFG22_09805"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET6416580.1"
                     /translation="MDITDANPAAEVPYSCRAATCGTCRVEVVAGGDALSTPDEFELE
                     VLEIFGDTPDRVRLCCQARIVGGADGPVVLKVCDD"
     gene            complement(257..754)
                     /locus_tag="VFG22_09810"
     CDS             complement(257..754)
                     /locus_tag="VFG22_09810"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HET6416581.1"
                     /translation="MSSDIVKIKPKPQGLDKPYVGKIIKTMSKVNVAIYRWTGGLLGS
                     TWRVGSAFPRGIPVLLLTTIGRKSGQPRIAPLIFIEDGGNVIIVGSQGGLPKDPLWYK
                     NLQANPDCEVQIKRRKMKMRAHTASPEERARLWPKLVEHYADFASYETWTDRVIPVVV
                     LEPVA"
     gene            complement(762..1592)
                     /locus_tag="VFG22_09815"
     CDS             complement(762..1592)
                     /locus_tag="VFG22_09815"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6416582.1"
                     /translation="MGHRTALITGAGGGLGHATVERLVANGWKVFAADISTDLLRSSM
                     HDPDVVPILMDVTDRESIKSAYETISSHTDRLDGVVNFAGVIGLGSLTDIPEERLARI
                     LDINVMGTFRVNKKFLPLLEAAKGRIVNISSETGWQSAAPFNGPYAMSKYAIEAYSTA
                     LRRELALLGIRVITVQPGPFRTDMVAGIERSFTQAENETQKFASVIRALKGLAAKQIN
                     SAHDPDILAQVIETALTAKRPKPVYSVKPDRLRSSLEKLPLGVIDRVYATVLRRAYKE
                     "
     gene            complement(1597..3177)
                     /locus_tag="VFG22_09820"
     CDS             complement(1597..3177)
                     /locus_tag="VFG22_09820"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET6416583.1"
                     /translation="MTNERLFDMPLMPHLVIDGLNRYDERPFLILGDATITYAEVRAD
                     ASRYAQALESKGIRKGSRTAVLSANRPEVLYNLCANMLVGSCNTPLHPLGSAEDQAYV
                     LRDAEIETLVFDPAYFGQRAAELKTRVPGLKHLLAFGPTEVGEDLNALAASFDPKPLV
                     APDIRPDDLNTIVYTGGTTGRPKGVRQPYRSAAYMTMVQMAEWEWPKEVRFLIATPLS
                     HAAAAFVVPTLQSGGAFVALQGFTPDLFFDAIEKHKITSTFLVPVMIYPLLDHPRAST
                     ADLTSLETLFYGASPMSPARLKEGVEKWGQIFFQCFGQSEAPMALTHLKKADHDLSKP
                     ERFASCGRPSPWVHLALLDESNQPAPDGEAGEICVRAPLVMEGYNGLPEQTAEAFAGG
                     WLHTGDVGRFDDDGFLHIVDRKKDMIVTGGFNVFPREIEDVISAHPAVAQVAVVGVPD
                     ERWGEAVKAVVILRPGHDPSDDLAAELKSAVKDAKGSVQSPKTIDFVASIPLSALGKP
                     DKKALRKQFWGEQTRGVN"
     gene            complement(3174..4097)
                     /locus_tag="VFG22_09825"
     CDS             complement(3174..4097)
                     /locus_tag="VFG22_09825"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6416584.1"
                     /translation="MTESFAPPVPLLRFELPNGMSIAADAHGDPRNQPVLFLHGGGQT
                     RHAWGRTAETLAGHGFYTICTDHRGHGESSWAGEGQYRLDHFVEDLRGVVAQLARKPI
                     LVGASLGGIASLLAQTEGDRSVAAGLVLVDVTPRMEADGVDRILDFMRGGTAGFASLE
                     EAADTIAKYLPHRKRPKDLSGLAKNLRRGSDGRYYWHWDPQMLKTWDPDRYTEDDDRT
                     VKARLQNVRGLDIPTLLIRGRLSDLVSAEAAAEFLEMVPHAEYVDLAGAHHMVAGDRN
                     DAFTDSITEFLLRRFTPEGAGNEDRVQRTSQ"
     gene            complement(4102..4737)
                     /locus_tag="VFG22_09830"
     CDS             complement(4102..4737)
                     /locus_tag="VFG22_09830"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6416585.1"
                     /translation="MSRAEIAQRQSGTRDLILESAERLFAERGFAATSIRDIVREAEL
                     TAPSLYHFFGSKENLLVELINVRHAAYCDELEADLEQAKSSFEVCQRFVRFVLTNMTK
                     RPTTAKFLLGIMFGPRLNIPEDVVGETLLRSQQILQARLRDVAVGLSESRIVFARMML
                     LGMVTPAVLMFLTAGVQSFSSKLPACIAYRVVEMMTDSHPTCAWPSVGLQS"
     gene            complement(4737..5537)
                     /locus_tag="VFG22_09835"
     CDS             complement(4737..5537)
                     /locus_tag="VFG22_09835"
                     /inference="COORDINATES: protein motif:HMM:NF005864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HET6416586.1"
                     /translation="MSEPHFLYEKRGHIAICTFNRPEKKNALSIEMLVRMADAWDEAD
                     ADAEVRAIILTGTGGVFCAGSDLGSMKSGKWDADDAWLNRMKESPDIHWKALLRHYLT
                     KKPLIAAVEGYALAGGTEILQGTDIRVAGEGAVFGVTEVQLGLFPLGGSSVRLRRQIP
                     YTIAAEMLLAGRRLTAAEALHYGLIGHVVPDGRALEKAMEIGERVAANGPVAVQAVKQ
                     SLIENDGVPAKEALARSLEIGQPVFKTRDAIEGPTAFMEKRKPQFQGS"
     gene            5660..6736
                     /locus_tag="VFG22_09840"
     CDS             5660..6736
                     /locus_tag="VFG22_09840"
                     /inference="COORDINATES: protein motif:HMM:NF013255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET6416587.1"
                     /translation="MPTVEPLEDLGVCLVTGAAGFLGSHLVRGLLDQGYSVRAVVRRT
                     ALPLEHPELEIVRGEMEDAERMMEICRGVDTVFHAAAHLALLGGSAASAKYRKAAYEA
                     NVRGVENLIAACQANGVSRFVHTSSIDVCFNSEVDTEMNELTPYASNMSCLYTETKIE
                     GEKRVLEANGQGGLLTTVLRPDGIYGPGNNLMLDEMFEQVTKGRLVATIGYPGALHDH
                     IYIDNLVDAEIRIAKRLVAGSPVCGKAYFVTDGRPLPLFEFMRPLIEGMGYKVPKLDI
                     PYRPVLAFLRLWQYLHFRIGLPAPFFSPHEIRKLAISHCASSAAAERDFDYRPIVSPD
                     DAMARCLAHYRERLASVRALAPPK"
     gene            6821..8614
                     /locus_tag="VFG22_09845"
     CDS             6821..8614
                     /locus_tag="VFG22_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416588.1"
                     /translation="MRRSFVLAHLLPLALILGCGSDDSSTQKCSADSTLGVVQSIFET
                     RGCTASSCHGADAEEASANLDLRPDHLYENVVNVRAGTGDLPLVFPADEERSVLYLKL
                     AAKTLDTELSDFGISGGPMPATADALTEDELAVVRAWIRGGAPRVGVVKDAPDVLGCG
                     SELEATPNKIPPLEPPPADKGIQFYSGGWKLPAESENEVCFVTYYDYSDRVPAEVRLP
                     CPDAYGGPTRECFGYSNFLLAQDPQSHHSIVETYIPPSDKQEEWDPGYEGWKNWQCLD
                     GANDGMPCDPTIEGVCGERSTCATAPLTALGCTAYQNAPPDLGTLLGFFGQASTRQNI
                     AVAQEATFIEDYAPGVYGVMPLKGFTIWNSHSFNLTKQDTTVEQYMNFTYSRPEDRKY
                     QREDLTVLNDIFAMGTVPPFTSQQACATFTLPVGARLVTLSSHTHQFGRDFRIWYPPN
                     EPCAAGPGCPAPTREPDYQSFFYQDPLYQRFDESNNLELDSAETNDRTFRYCAIWDNG
                     ATDPEEVRRHSNRPDANTCVFGDLVGFVGQCGCPPEQRACLGGPNQGAICNGDDAACG
                     EGGVCDACPVWGGVTTEEEMFGILGAYYVVD"
     gene            8940..9013
                     /locus_tag="VFG22_09850"
     tRNA            8940..9013
                     /locus_tag="VFG22_09850"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8974..8976,aa:Ile,seq:gat)
     gene            9025..9100
                     /locus_tag="VFG22_09855"
     tRNA            9025..9100
                     /locus_tag="VFG22_09855"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9058..9060,aa:Ala,seq:tgc)
     gene            9510..12514
                     /locus_tag="VFG22_09860"
     rRNA            9510..12514
                     /locus_tag="VFG22_09860"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            12644..12760
                     /gene="rrf"
                     /locus_tag="VFG22_09865"
     rRNA            12644..12760
                     /gene="rrf"
                     /locus_tag="VFG22_09865"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(12855..13235)
                     /locus_tag="VFG22_09870"
     CDS             complement(12855..13235)
                     /locus_tag="VFG22_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416589.1"
                     /translation="MYGTMAKLILFGALLLGVGACKTAPTEQDEAARIVDPDDASRAA
                     LQRVVDDALHTNVNLANDALTKSSILIIHRKPMRDLNGIPIDGRRLEAPIQFRLVTNR
                     EHCILIDQRDQSRYELENTRCEPE"
     gene            complement(13235..14806)
                     /locus_tag="VFG22_09875"
     CDS             complement(13235..14806)
                     /locus_tag="VFG22_09875"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HET6416590.1"
                     /translation="MRVSILAMGLASCGDSGGAAWPGSGGTGGEWQPGVFQPASVFES
                     QCEEPRVGVNPFTDQPYSDVQGTITDENNFLRSYSDDTYLWYDEINDQDPALFDDQIL
                     YFNQLKTNALTSSGQPKDKFHFSIDVQEYLSLSQSGVSAGYGVQWALLSAVAPREILV
                     AYTEPDSPATNPQVALERGAKVLAIDGFDIDTDTDAGIDALNAALFPSEAGATHTFTI
                     QDRDSQDSRSITMTSAFIESEPVKNVRVLETPTGRVGYILFNDHIATAELGLFEAVNQ
                     LNEGEGIDDLVLDMRYNGGGFLALASELAYMIAGPAATDGRAFELIEFNDKHPDTNPV
                     TGRKISPTPFYDTTLGSPFNAPEDQPLPTLNLPRVFVLTSGSTCSASEAVMNGLRGID
                     VEVIQIGTTTCGKPYGFYATDNCGTAYFTIQFRGVNDKDFGDYTDGFSPANTEGTMGV
                     SIPGCSVADDFGAELGDLNEDRLAAALSYRETQTCPEPPGLASGFASKSGDGRDDTDA
                     LVPKSLWQTNRTMLP"
     gene            complement(14911..16179)
                     /locus_tag="VFG22_09880"
     CDS             complement(14911..16179)
                     /locus_tag="VFG22_09880"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET6416591.1"
                     /translation="MRIIGLFVVPLLVLGACSSSDPEAQDVTIDTFNLALAGAFIPYE
                     AERRQPLADAIAEMDSDIVCLQEVWTQADKEMIRAAAATNFPNAVLFANDLDTPLDDP
                     EDQNGDDPPAPTTVPCPPIEVLPGVTVEDQMSLAIECVSENCNTIAPGDQDGQTTSTE
                     CAQDFCLGEVAALILGDPQQLRCYSCLATQLPTSTFAEIGSSCPVIINQDLAFEGQNG
                     VMILSRYPLKDPVNWVIPGTWNRRVIISATAELPNGSELDVYCNHLTPIFDSIAYPYT
                     GQYGDGLTGAEGWEAEQQLQAKKLIDHVAATSGDTPAVILGDLNAGRAYPDESLVAEG
                     AATLERLEAAFTPAYTESYEPQCTFCADNPLTDTTSSVWIDHILLNGLTSDAVQSTVR
                     TFDEAVVPVGGDELVPLSDHYGLRSVIRIP"
     gene            complement(16318..16761)
                     /locus_tag="VFG22_09885"
     CDS             complement(16318..16761)
                     /locus_tag="VFG22_09885"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HET6416592.1"
                     /translation="MASRYRHLRARALKAMFKTHAAIYERSGGRLGAWLGLPTLLLSV
                     TGRKSGKTFATPLVYFKDGESYVVVGSDGAAKHDPQWWKNLQANPACVVRVGRRKLAA
                     KAALASGADRERLWQQGKTVNPMWSKYQARTERELPVVVLTPTHD"
BASE COUNT         3413 a         4973 c         5180 g         3371 t
ORIGIN      
        1 atggacatca ccgacgccaa tcctgcggcg gaggttccct actcttgccg cgcggcgacc
       61 tgtggcacct gcagagtcga ggtcgttgcg ggcggcgacg cgctctccac gccggacgaa
      121 ttcgagctcg aagtcctcga gatcttcggg gacacgccag atcgggtccg cctctgttgc
      181 caagccagaa tcgttggcgg cgccgacggc ccagtcgtcc tgaaggtttg cgacgattga
      241 acgccgagag aggacctcag gcgaccggct cgagaacgac caccgggatc acgcgatcgg
      301 tccaggtctc gtaacttgcg aagtcggcgt agtgttcgac gagcttcggc cagagacggg
      361 cgcgttcctc gggtgaagcg gtgtgggctc tcatcttcat cttgcgccgc ttgatctgaa
      421 cttcgcagtc cgggttcgcc tggaggttct tgtaccagag cgggtccttc ggaagaccgc
      481 cctgtgagcc cacgatgatg acgttccccc catcttcgat gaagatgagc ggcgcgattc
      541 tcggctggcc gctcttgcgc ccgatcgtcg tgagcagcaa gacggggatg cctcggggaa
      601 acgcgcttcc aacacgccaa gtcgagccca gcaatccgcc ggtccagcgg tagatcgcca
      661 cgttcacctt cgacatggtc ttgatgatct tgccgacata gggcttgtcg aggccctggg
      721 gcttgggctt gatcttcacg atatcgctgc tcatgccgtg tctattcctt gtatgcccgc
      781 cgtagcacgg tcgcgtaaac gcgatcgatc acaccgagcg gcaacttttc gagagacgac
      841 ctcaaacggt ccggcttcac cgagtagacc ggctttgggc gcttcgccgt gagcgcggtc
      901 tcgatcacct gggccaagat gtctggatca tgcgcggagt tgatctgctt ggcagccaag
      961 cccttgagcg cgcgaatcac cgaagcaaac ttctgggtct cgttctcggc ctgcgtgaac
     1021 gatcgctcga tccctgccac catgtcggtg cgaaacgggc cgggctgaac cgtgatcacc
     1081 cgaatgccga ggagcgccag ctcacgccgt agcgcggtcg aatacgcctc gatcgcgtac
     1141 ttgctcatcg cgtagggccc attgaagggc gccgcgcttt gccagccggt ctccgagctg
     1201 atgttgacga ttcgcccctt cgccgcttcg agcagaggca agaacttctt gttgacccgg
     1261 aacgtgccca tcacgttgat gtcgagaatt cgcgcgaggc gctcctcggg gatgtcggtc
     1321 aacgagccga gtccgatcac cccggcgaaa ttcaccacac cgtcgagacg gtcggtatgg
     1381 ctggaaatgg tttcgtaggc gctcttgatg ctctcgcgat cggtcacgtc catcaggatc
     1441 ggcacgacgt ccgggtcgtg catcgagctc cgaaggaggt ccgtgctgat gtccgcggcg
     1501 aagaccttcc agccgttggc aaccaagcgt tcgacggtgg cgtgacccag tccgcctcct
     1561 gctccggtga tcaatgcggt tcgatgaccc atgggctcag ttgactccac gcgtttgctc
     1621 gccccagaac tgcttacgca gcgccttttt gtcaggcttg cccagcgctg aaagcggaat
     1681 ggatgccacg aagtcgatcg tcttcggaga ctgcacggac cccttggcgt ccttcacggc
     1741 agacttgagc tcggcggcca ggtcatcgct ggggtcatgc cccgggcgga gaatcacgac
     1801 cgctttcacg gcttcgcccc agcgctcatc gggcacgccg acgaccgcca cctgtgccac
     1861 cgcaggatgt gccgagatca cgtcttcgat ctcgcgcggg aacacgttga acccacctgt
     1921 gacgatcatg tccttcttcc ggtcgacgat gtgaagaaag ccgtcgtcgt cgaagcgtcc
     1981 gacatcgcct gtgtgaagcc agcctccggc aaaggcctcg gcggtttgtt cggggagtcc
     2041 gttgtaaccc tccatcacga gcggtgcgcg cacgcagatc tccccggctt ctccatcggg
     2101 agcgggctga ttgctctcat cgagaagcgc cagatgcacc cagggacttg gacgtccgca
     2161 ggaggcgaaa cgctccggct tcgaaaggtc gtggtcggcc ttcttcaagt gcgtcaaagc
     2221 catcggcgct tcggattgcc caaagcattg gaagaagatc tgcccccact tctcgacgcc
     2281 ttccttgaga cgtgccgggc tcatcggtga ggctccatag aacagcgttt cgaggctcgt
     2341 gaggtccgca gtgctggccc gcggatgatc gagcagcggg tagatcatca caggaaccaa
     2401 aaaagtcgag gtgatcttgt gcttttcgat cgcgtcgaag aacagatccg gagtgaaccc
     2461 ctgcaaggcc acgaatgcac ctccgctctg caaggtcggc acgacgaatg cggcagctgc
     2521 atgcgaaagc ggcgtcgcga tcaggaaccg cacctccttc ggccactccc actccgccat
     2581 ctgaaccatc gtcatgtaag ccgccgagcg atacggctgg cgcacgccct tcggacgccc
     2641 cgtcgtaccg cccgtgtaga cgatcgtatt gagatcgtcg ggcctgatgt cgggcgcgac
     2701 gagcggcttg ggatcaaagc ttgcggccag ggcgttcaag tcctcgccga cctcggttgg
     2761 gccgaacgcg agaagatgtt tcaaacccgg cacgcgggtc ttgagctcgg ccgcccgctg
     2821 cccgaagtaa gcaggatcga aaaccagtgt ttcgatctcg gcgtctcgta gcacgtaagc
     2881 ctggtcttcg gccgatccga gcggatggag tggagtgttg caagacccaa cgagcatgtt
     2941 ggcgcagagg ttgtagagaa cctccgggcg gtttgccgaa agcaccgccg tgcgcgagcc
     3001 cttgcgaatc cctttggact cgagcgcctg cgcatagcgg ctcgcgtcgg cccgcacctc
     3061 tgcatacgtg atcgtcgcgt cccctagaat caggaacggc cgctcgtcgt agcgattgag
     3121 tccgtcgatc accaggtgcg gcatcagggg catgtcgaaa agtcgttcgt tcgtcattgg
     3181 gaggtccttt gtactcgatc ttcgtttcca gcgccctcgg gcgtaaagcg acgaagtagg
     3241 aattcggtga tgctatcggt gaacgcgtcg tttcggtcac ccgcgaccat gtggtgtgca
     3301 cccgcgaggt cgacgtactc cgcatggggc accatctcga gaaactcggc agcggcttca
     3361 gccgacacca agtcgctcag gcggccacga atcagcaagg tcgggatgtc gaggccgcgc
     3421 acgttctgca ggcgcgcttt gaccgtgcgg tcgtcgtcct ccgtgtacct gtctgggtcc
     3481 caggtcttga gcatctgcgg gtcccagtgc caatagtagc gcccgtcgct tccgcgcctc
     3541 aggttcttgg ccagccccga aaggtccttt gggcgttttc tgtgtggcag gtattttgcg
     3601 atcgtgtctg ccgcttcttc gagcgaggcg aagcccgctg tgccgccccg catgaaatcg
     3661 aggatccgat cgactccgtc cgcttccatg cgcggcgtca cgtcgaccag taccagcccc
     3721 gcggcgaccg aacgatcgcc ttcggtttgc gccaagagag aggcgatccc gccgagtgac
     3781 gcgccgacca gaataggctt ccgcgccaac tgtgcgacca cccccctgag gtcctcgacg
     3841 aagtggtcga ggcgatattg accctcgccg gcccaactgc tttcgccgtg gccgcggtgg
     3901 tcggtgcaga tggtgtaaaa cccgtgccca gccagcgtct ctgcggtgcg cccccaggca
     3961 tgtctcgttt gacccccgcc gtgcaggaac agcactggct ggttcctcgg atctccgtga
     4021 gcgtccgccg cgatcgacat cccattgggc agctcgaatc gcaggagagg aacaggtggt
     4081 gcaaaagact cggtcatcgt ctcaggattg cagccccacg ctaggccacg cgcaagttgg
     4141 atgtgagtcc gtcatcattt cgacgacgcg gtacgcgatg caagccggca actttgacga
     4201 aaaggattga acgccagcgg tgagaaacat gagcaccgcg ggcgtcacca tcccaaggag
     4261 catcattcgt gcaaacacga tgcgcgactc cgacaggcca acggcgacgt cacgcagccg
     4321 cgcctggaga atctgctgcg agcgtaggag cgtttcacca accacgtctt cagggatgtt
     4381 gagccggggc ccgaacatga tgcccagcag gaacttcgcg gtagtaggac gcttcgtcat
     4441 gttggtgaga acgaagcgaa cgaagcgctg gcacacctcg aacgagctct tggcctgctc
     4501 gaggtcagct tcgagctcgt cacagtacgc cgcgtggcga acgttgatca gctcgaccag
     4561 gagattctct ttgctgccga agaagtgata gaggctcgga gccgtgagct cggcttcccg
     4621 cacgatgtcg cgaatgctcg tggctgcgaa gccccgctcc gcaaagaggc gttccgcgct
     4681 ctcgagtatg agatcccgcg tgccgctctg gcgctgcgct atttcggctc gagacatcag
     4741 gagccttgaa actgcggctt tcgcttctcc atgaaggctg tagggccttc gatggcatcc
     4801 ctggtcttga agaccggttg tccgatttcg agtgagcgcg ccagcgcttc tttggccggc
     4861 acgccgtcgt tctcgatgag cgattgcttg acggcctgca cggcgaccgg gccgttcgca
     4921 gcaacgcgct cgccgatctc cattgccttc tcgagcgctc gtccgtccgg caccacgtgc
     4981 ccgatgagcc cgtagtgcag tgcctcggcg gctgtgaggc gacgtcccgc aagcagcatc
     5041 tcggcggcaa tcgtgtaggg gatctgccgg cggagtcgca cgctcgaacc tcctaggggg
     5101 aacagaccga gctgaacctc cgtgacgccg aacacggcgc cctctccggc gacacgaatg
     5161 tcggtgcctt gaaggatctc cgtgccaccg gcgagcgcat acccctccac tgctgcaatc
     5221 aaaggcttct tggtgaggta gtgccggagg agcgccttcc aatggatgtc cgggctctct
     5281 ttcatacggt tcagccaagc gtcatctgcg tcccacttgc ccgacttcat cgagccgagg
     5341 tcactgcccg cgcaaaatac gccccccgtc ccggtgagaa tgatcgcgcg aacttccgcg
     5401 tccgcatcgg cctcgtccca ggcgtccgcc atccgcacca gcatctcgat gctcagcgcg
     5461 ttcttcttct cgggccggtt gaaggtgcag atcgcgatgt gaccgcgctt ttcgtagagg
     5521 aaatggggtt ccgacataga agcttctccg tagctcatcc aagctcccgt ggctataacg
     5581 aacgttacat atacagtcaa gccctggcac gcttggcaga cttgctcctt cgcggccccg
     5641 tgctccaatc gccaccacga tgccgaccgt tgaaccgctc gaagatctcg gagtctgtct
     5701 cgtcaccggg gcggccggtt ttctcggcag ccacttggtc cgcggtttgc tcgaccaggg
     5761 ctactcggtt cgtgccgtcg ttcgccgcac ggcgttgcct ctcgaacacc ccgagctcga
     5821 gatcgtgcgc ggcgagatgg aagacgccga gcgcatgatg gagatctgtc ggggcgtgga
     5881 caccgtgttc cacgcggcgg cacacctcgc gctgctcggt gggagcgcgg cctccgcaaa
     5941 gtatcgtaag gccgcgtacg aagcgaacgt gagaggcgtc gagaatctca tcgccgcgtg
     6001 tcaagcgaat ggggtcagcc gcttcgtaca cacgagctcg atcgacgtct gcttcaactc
     6061 cgaggtcgat accgagatga acgagctgac gccctatgcc agcaacatga gctgcctcta
     6121 caccgaaacg aagatcgaag gcgaaaagcg ggttctggag gccaacggcc agggcggctt
     6181 gctgacgaca gtcctaaggc ccgatgggat ctacggtccc ggcaacaacc tcatgttgga
     6241 cgagatgttc gagcaggtga ccaagggccg actcgtcgcg acgatcggct atcccggtgc
     6301 gcttcacgat cacatctaca tcgacaacct cgtcgacgcc gagatccgca tcgccaaacg
     6361 cctggttgcc ggcagtcccg tttgcggcaa ggcgtacttc gtcaccgacg ggcgcccgct
     6421 cccgctgttc gagttcatgc ggcctttgat cgaaggcatg ggctacaagg ttccgaagct
     6481 cgacattccg tatcgcccgg tgctcgcgtt ccttcgattg tggcaatacc tgcactttcg
     6541 cattggcttg ccggcgcctt tcttcagccc tcacgagatc cgcaagctcg cgatttcaca
     6601 ttgcgcgagc agcgcggcag ctgagcgcga cttcgactac cggccgatcg tcagcccgga
     6661 cgacgcgatg gctcgatgcc tggcgcacta ccgcgaacga ctggccagcg ttcgcgcgct
     6721 cgcgcccccg aaatagagcc cgatccattg acgcaggcgg ccgatacggt tatccccgac
     6781 ccgccgctcg accccgtttg tggagtgaag gagacgcccc atgcgccgct cgttcgtact
     6841 cgctcatctg ttgcccctcg ctctgatcct tggctgcggg agcgacgact catcgactca
     6901 gaaatgcagt gcggacagca cccttggtgt ggtgcagtcg atttttgaaa cccgaggttg
     6961 cacggcgagc agctgtcatg gcgccgatgc cgaagaagct tcggccaatc tcgatctccg
     7021 accggaccat ctctacgaga acgtggtcaa cgtccgagct gggacagggg atcttcctct
     7081 cgtcttcccc gccgatgaag agcggagcgt gctctacctc aagctcgcgg cgaagacgct
     7141 cgacaccgag ctttcggact tcgggatctc gggcggcccg atgccggcga cggccgatgc
     7201 gctcaccgaa gatgagctcg cggtggttcg cgcctggatt cgcggcggag caccgcgcgt
     7261 cggcgtcgtg aaggacgcac ccgacgtgct cggttgtggt tcggagctcg aagccactcc
     7321 caacaagatc ccgccgctcg agccgccgcc tgcagacaaa ggcatccagt tctattccgg
     7381 aggctggaag cttcccgccg aatcagagaa cgaagtctgc ttcgtcacct actacgacta
     7441 tagcgaccgt gtgcctgccg aggttaggct gccatgcccc gatgcctatg gcggaccaac
     7501 ccgcgaatgc ttcggatact cgaatttcct actcgcgcag gacccgcaat cccaccactc
     7561 gatcgtcgag acgtacatcc ctccctccga taagcaggaa gaatgggacc cgggctacga
     7621 aggttggaag aactggcagt gtctcgacgg agccaacgat ggcatgccct gcgaccccac
     7681 gattgagggc gtctgcggcg agcgcagcac ctgtgcgact gcaccgctca ccgcgctcgg
     7741 atgcaccgcg tatcaaaacg cgcctccaga cctcggcacg ctcctgggct ttttcggaca
     7801 agcatcgacg cgccagaaca tcgcggttgc gcaagaagcg acgttcatcg aggactacgc
     7861 gcccggcgtc tatggcgtga tgcccctcaa gggcttcacg atctggaaca gccactcgtt
     7921 caacctcacc aagcaggaca ccacggtcga gcagtacatg aacttcacgt acagcagacc
     7981 agaagaccgc aaataccagc gcgaagacct caccgttctc aatgacatct ttgcgatggg
     8041 caccgtgcct ccgttcacga gtcagcaggc ctgcgcgacg ttcaccttgc ccgtaggtgc
     8101 gcgcttggtg accttgtcct cgcacacgca ccagttcggg cgagatttcc gaatttggta
     8161 tccgccaaac gaaccatgcg cagcaggccc gggttgcccg gcgccgaccc gcgaacctga
     8221 ctaccagagc ttcttctatc aggacccgct ctaccaacgc ttcgacgaga gcaacaacct
     8281 cgagctcgac agcgctgaaa cgaacgatcg cacgttccga tactgcgcga tctgggacaa
     8341 cggcgcgacc gatccagaag aagtccgcag gcattccaac cggcccgacg ccaacacgtg
     8401 cgtgtttggg gatctcgtcg gtttcgtagg gcagtgcggc tgccctcccg agcagcgagc
     8461 ctgcctcggc ggcccaaatc agggtgcgat ctgcaacggc gatgatgcgg cttgcggcga
     8521 aggcggcgtc tgcgatgcct gcccagtctg gggcggcgtg acgaccgaag aagagatgtt
     8581 cggcattctt ggcgcctact acgtggtcga ctgaattatt atataattct aagtagttac
     8641 tggcatgtcc gagaagaggg acataggcgc gcttcaaggc gcccccgagg aggatcaagc
     8701 gaactaccta ggcgccggaa gcgcttgaga aatggcctgt tctcctcttt tggaaaagct
     8761 tctttgaaag tgcttgacgg gtttggggaa ggccgctaaa gaaggcgtcc ctttgcggat
     8821 gaagttgggg acgggaacca acaaaacgga gtagttttca gggcttaggc ccgaaccagc
     8881 ttcgaaaacg gttcacggaa acagcgtctg acgctctttg acagtttgcg agaatacacg
     8941 gggcactagc tcaggtggtt agagcgcacc cctgataagg gtgaggtcgc tagttcgagt
     9001 ctagcgtgcc ccactaaata ggacggggct gtagctcagc tgggagagcg ccggctttgc
     9061 aagccggaag tcaggggttc gatccccctc agctccacca cgttacaccc acccccgccc
     9121 ccgggctcct ccacggaccc tccgggtccg ggattggata gccgagtgtc gactcggcac
     9181 ttgccaagac acccgtgaag acccagtctt gattccttcg gtcggcgacg cgaaagcacg
     9241 cgtcttcgac ccgagtaatc aagattcaaa gctcgattct cgcagctctt tgacaactga
     9301 atatctgtag aaattttctc tttctagccg tagctgtgac caggctcttc tttcgaaccc
     9361 tggtctcgag ttacgaaggt gctgagccat aaagactttc cgtcatggcg aagcacccgg
     9421 tgtggaactg ggtatcgcta tccaggaaag caccaactta gcgcttcgat ttgcgtcgaa
     9481 gcgccgagac gaaatgcgtc ttaggaaaag gtcaagctag gaagggcaca cggtggatgc
     9541 cttggcgtcg agaggcgatg aaggacgtgg gcagctgcga aaagcttcgg ggagtggcta
     9601 acacactttg atccgaagat atccgaatcg ggaaaccgtc tggggttata cccagaagtc
     9661 caacgctgaa ttcataggcg ttggatagcc aacccaggga actgaaacat ctaagtacct
     9721 ggaggaaaag acatcaaccg agactcccct agtagcggcg agcgaacggg gactagccta
     9781 aaccatatca atgtaagctt aggggcgttg ttgatatggg gtcgtgggtc cagtggtgga
     9841 gcgccctaat gctccgacag agttacaaag ccaaacgtta gatgaacgat ctggaaaggt
     9901 cggcgataca aggtgacagc cctgtagtcg aaaacggttg gtctctgcac tggtacccga
     9961 gtaccacggg gcacgtggaa tcctgtggga atctgggagg accatcttcc aaggctaaat
    10021 actccttgtc gaccgatagt gaactagtac cgtgagggaa agatgaaaag tactcctgtg
    10081 aggagagtga aatagtacct gaaaccgtgt gtctacaagc agtcgaagca ctatgccctt
    10141 cggggaatgt gcgacggcgt accttttgca taatgggcct acgagttacg ctttgttgca
    10201 aggttaagcc gataggtgaa gccgtaggga aaccaagtcc aaatagggcg atttagtagc
    10261 agggcgtaga cccgaaaccg agcgatctat ccttgggcag gttgaagcgt gggtaacact
    10321 gcgtggagga ccgaacccac tgaagttgaa aattcagggg atgacctgag gatcggagtg
    10381 aaaggctaat caagctcgga gatagctggt tctccgcgaa atatattgag gtatagcgtc
    10441 tagtgaattg ccccgggggt agagcactgg atgggctagg ggtcctacca gattaccaaa
    10501 cccaaccaaa ctccgaatac cggtgacaac tgctaggcag tcaggctgcg ggtgataagg
    10561 tccgtagcca agagggaaag agcccagatc gacagctaag gtccctaatc cagactaagt
    10621 gtgaaaggat gtgggggcgc tcagacagcc aggaggttgg cttagaagca gccatccttt
    10681 aaagaaagcg taacagctca ctggtcgagc gaacccgcgc cgaaaatgta acggggctaa
    10741 gtctggtacc gaagcttcgg gttctaattt attagagcgg tagcggagca ttctcacgca
    10801 gatacaaggt gtaccgtaag gagcactgtc gggtgtgaga agagcttatg taggcatgag
    10861 tagcgataaa cagggtgaga aaccctgtcg ccgtaagccc aaggtttccc aggcaagaat
    10921 aattcgcctg tgggttagtc ggcccctaag tggagcccga aaggggtaca cgatgggaag
    10981 caggttaata ttcctgcacc actgaggaat ccgttgatct aagcagggac ggagaaggat
    11041 aggccgatcg ggcgaatgga agtgcccgtt caagcaggta ggaggtttcc gtaggaggcg
    11101 atagccacaa acgcggaaac attactccga gacgtgatga ggtggagctc cggctccgta
    11161 actcggtgat tccatgcttc caagaaaagc tgcgtagtga gggtcttcag tgaccgtacc
    11221 gcaaaccgac tcaggtgggc ggggtgagaa tcccaaggcg cttgagagaa cactggctaa
    11281 ggaactcggc aaattgacac cgtaacttcg ggagaaggtg tgcccgtgtc cgtgatgaga
    11341 cttgctctct gagcgggtgt gggttgcaga gaatagaggg ttgcgactgt ttaccaaaaa
    11401 cacaggactc tgcgaactcg taagaggacg tatagggtct gacgcctgcc cggtgctgga
    11461 aggttaagag gagttgttag cttcggcgaa gcagcgaatc gaagccccag taaacggcgg
    11521 ccgtaactat aacggtccta aggtagcgaa attccttgtc gggtaagttc cgacctgcac
    11581 gaatggcgta acgacatccc tactgtctcg gccagtgact cagcgaaatt gtattgagcg
    11641 tgaagatacg ctctacccgc gacaagacgg aaagaccccg tgaaccttta ctgcagcttg
    11701 gcagtgaagt tcgggacagt ctgcgtagga taggtgggag acttcgaagc cgggattctg
    11761 gtttcggtgg agtcaacctt gaaataccac cctggctgtt ctggacttct aacccagcac
    11821 cgtgatccgg tgcggggaca ttgcctggtg ggcagtttga ctggggcggt cacctcctaa
    11881 aatgtaacgg aggtgcgcga tggttccctc aggctgattg gaaaccagcc gtagagtgca
    11941 aaggcataag ggagcttaac tgtgagaccg acaggtcgaa cagatgcgaa agcaggcctt
    12001 agtgatccgg taactccata tggaagggtt atcgctcaac ggataaaagg tactccgggg
    12061 ataacaggct gatcgcgcct gagagttcat atcggcggcg cggtttggca cctcgatgtc
    12121 ggctcatcgc atcctggggc tggagcaggt cccaagggtt tggctgttcg ccaattaaag
    12181 cggtacgcga gctgggttca gaacgtcgtg agacagttcg gtccctatct gtcgtgggcg
    12241 gaggatactt gaggggagtt gaccatagta cgagaggacc tggttgaacg tatccctagt
    12301 gttccggttg tttcgccaga agcatagccg ggtagctata tatggaaccg ataaccgctg
    12361 aaagcatcta agcgggaagc gggccccaag atgaggtatc cctggacttc ggtccctaaa
    12421 ggccactcgg agactacgag tttgataggc tgggtgtgga agcgtggcaa cacgtggagc
    12481 tgaccagtac taatcggccg tgaggcttga ccttcttcta agacacgttt cgtgcgtgat
    12541 ttgagatcag ggttggaagg aagagctcga gacaatccat caacacggac tctcggactc
    12601 gcttctgatc cgcacagagc ggcacagaga aactttctac ggattcggtg gcaatgtcgg
    12661 tagggtcaca cccgatccca tcccgaactc ggaagttaag cctatcagag ccgatggtac
    12721 tgcaggggca gccctgtggg agagtaggac gccgccggaa ttatgaaagc ccgtctcgaa
    12781 agagacgggc tttcctcttt ttgccgatac gggctcgtga tctagccccc gcattgagct
    12841 ggccggccga cgagctattc gggttcgcag cgcgtgttct cgagctcgta acgggattgg
    12901 tcacgctggt cgatcaggat gcagtgttca cggttggtca ccaggcggaa ctggatcggg
    12961 gcttcaaggc gacgaccatc gattgggatg ccgttcaagt cgcgcatggg cttccggtgg
    13021 atgatcagga tgctgctctt ggtcaaggcg tcgttcgcga ggttgacgtt cgtgtgcaat
    13081 gcgtcgtcga ctacgcgttg aagcgctgcg cggctcgcgt catcggggtc gacgatgcgg
    13141 gcggcctcgt cttgctcggt tggtgctgtt ttgcaggcgc cgactccgag caagagcgca
    13201 ccgaacagga tcagcttggc catcgttccg tacatcacgg caacatggtc ctattcgttt
    13261 gccaaagcga tttcggtacg agcgcgtccg tatcgtcccg gccgtcgccc gacttgctcg
    13321 cgaagcccga cgcaagcccc ggtggctccg ggcaggtttg agtctcgcgg tagctcaatg
    13381 cggccgcgag gcgatcttcg ttcagatcac cgagctccgc gccaaaatca tccgctacgg
    13441 agcagcctgg gatggaaaca cccatggtgc cctccgtatt ggcgggcgag aaaccgtctg
    13501 tgtagtctcc gaagtccttg tcattcacgc cgcgaaactg aatcgtgaaa taggccgtgc
    13561 cgcagttgtc ggtggcgtag aacccatagg gcttgccgca ggtcgtggtt ccgatttgaa
    13621 tcacctcgac atcgattcct cgcagaccat tcatgaccgc ttcgctggcc gaacaggtgg
    13681 acccgctggt gaggacgaag acacggggca ggttcagtgt cggaagcggt tggtcttccg
    13741 gcgcattgaa gggcgaaccg agcgtcgtgt cgtagaatgg cgtgggcgag atcttccgtc
    13801 cggtcaccgg attggtgtcg gggtgcttgt cgttgaactc gatgagctcg aaagcccgtc
    13861 catccgtggc ggccggccct gcgatcatgt aggcgagctc gctcgcgagg gcgagaaacc
    13921 cacccccgtt gtagcgcatg tcgagaacga ggtcgtcgat gccttcgcct tcattgagct
    13981 ggttcacggc ctcgaagagc cccagctccg cggtggcgat gtgatcgttg aacaggatat
    14041 agccgacgcg gccggtgggc gtttcgagca cgcgaacgtt cttcacaggc tccgattcga
    14101 tgaacgccga cgtcatcgtg atggaccggc tgtcctgcga atcgcgatcc tgaatcgtga
    14161 acgtatgcgt tgcgcccgct tcggacggaa acaaagcggc attgagcgcg tcgattccgg
    14221 cgtccgtgtc cgtgtcgatg tcgaaaccat cgatggccag aaccttggcg ccgcgttcga
    14281 gagcgacttg aggattcgtc gccggtgagt ccggctcggt gtaagcgacg aggatctcgc
    14341 gcggggcgac ggcagagagc aaggcccact ggacgccata gcctgcagac acaccggatt
    14401 gcgaaaggct cagatactct tgtacgtcaa tcgaaaagtg gaacttgtct ttgggttgac
    14461 cggaagacgt gagcgcgttt gtcttcagct gattgaagta gagaatctgg tcatcgaaga
    14521 gcgcggggtc ctggtcgttg atctcgtcgt accagaggta cgtatcatcg ctgtaagacc
    14581 ggaggaagtt gttctcgtcc gtgatcgttc cttggacatc cgagtagggc tgatccgtga
    14641 acggattgac gccgacgcga ggctcctcgc actgcgattc gaaaacgctc gcaggctgga
    14701 acactcccgg ctgccattcg ccacccgttc cacccgagcc cggccaggcc gcgccgccgc
    14761 tgtcaccgca ggaggccagc cccatggcca aaattgagac ccgcatgtag cgcgccattc
    14821 gtttggacac cccccggttt cccatcccta tttgaattac gcagatccac gcctctgtcg
    14881 actcttcttt aggtgtgggc aattacgaaa ctagggaatc cgaattaccg aacgcaagcc
    14941 gtagtggtcc gaaaggggaa cgagctcatc acccccgacg gggactacgg cttcgtcgaa
    15001 cgtgcgaacc gtggattgca ccgcatcgga tgtcaggccg ttgagcaaga tgtgatcgat
    15061 ccagaccgag ctcgtcgtgt cggtcaaagg attgtctgca cagaaggtgc actgcggctc
    15121 atagctctcg gtataagcgg gcgtgaacgc cgcctcgagc cgctctaagg tagcggcccc
    15181 ttcagcgacc aggctctcat cgggataggc gcgacctgca ttgaggtcgc cgaggatcac
    15241 cgcgggcgtg tcgccgctgg tggctgccac gtggtcgatg agtttcttgg cttgaagctg
    15301 ttgctcggcc tcccagccct cggcaccggt cagcccgtct ccgtactggc cggtgtaggg
    15361 ataggcgatg ctatcgaaga ttggggtcag gtggttgcag tacacgtcga gctcgcttcc
    15421 gtttggcagc tcggctgtag cgctgatgat gacacgtcgg ttccaggtgc ccgggatgac
    15481 ccagttcacc gggtctttta gcgggtagcg agagaggatc atcacaccgt tctgcccctc
    15541 gaaagcgagg tcttgattga tgatgacagg acagctcgag ccgatctccg cgaatgtgct
    15601 cgtgggaagc tgcgtggcga ggcaggagta acagcgaagc tgctgcgggt ctccgaggat
    15661 gagcgccgcc acctcgccca agcagaagtc ttgggcgcat tcggtgctgg tggtttgccc
    15721 gtcctgatca cccggcgcga tcgtgttgca gttctccgag acgcattcga tcgcgaggga
    15781 catctggtct tcgacggtga cgccgggcag cacttcgatc ggcgggcacg gcaccgtcgt
    15841 cggggcgggc gggtcgtctc cgttctggtc ctccgggtcg tcgagcggcg tgtccagatc
    15901 gtttgcaaaa agcaccgcgt tcgggaagtt cgtcgctgcg gcggcgcgga tcatttcttt
    15961 gtccgcctga gtccagacct cctgcaaaca cacgatgtct gagtccatct ccgcgatcgc
    16021 gtcggccagt ggttgccttc tttccgcttc gtacggaatg aacgccccag cgagggcaag
    16081 gttgaaggta tcgatcgtga catcttgggc ttcagggtcg ctcgaactac aggcgccaag
    16141 cacgagcaac ggcaccacaa acaaacctat gattcgcatc tagtcctcca ggtcggcgaa
    16201 cgccggcatc caagctcgac agcgtacttc gcgaatctgt cacaaagccg ggacaaaggg
    16261 aagggccgca gccgctcgga gcagctcgca tccacggtcg gtgctgaacg ggcctagcta
    16321 gtcgtgcgtc ggcgtgagca cgaccaccgg cagctcgcgc tcggttcggg cctggtactt
    16381 cgaccacatc gggttcacgg tcttgccctg ctgccagagg cgttcgcgat cggcgcctga
    16441 tgcaagcgca gcctttgccg cgagcttcct ccgaccgacg cgaaccacgc atgctggatt
    16501 tgcctgcagg ttcttccacc attgagggtc gtgtttcgca gctccgtccg agccgaccac
    16561 cacatagctc tcgccgtctt tgaaatagac caagggcgtc gcaaacgtct tgccggactt
    16621 ccgcccagtc acggagagaa gcaaggtcgg caatccgagc caggcgccga gacggcctcc
    16681 gctacgctcg tagatcgccg catgcgtctt gaacatcgcc ttgagcgcac gcgcccgaag
    16741 gtgcctgtag cgcgaggcca tcgacggacc cttacgcgtc gctcgccatg atgaaaagcg
    16801 gcaaaccgtt gaaggatgtg tacttcggtc gcattcggcc ggtgtcatag tacttttcga
    16861 cgtcgacgaa cttcttcgtt ccgatgatcg tttccatcgg cacactgccg tagcggccat
    16921 tgtgcacgga gacgagt
//
LOCUS       DASRZF010000222         4452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_840312, whole genome shotgun sequence.
ACCESSION   DASRZF010000222 DASRZF010000000
VERSION     DASRZF010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 4452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4452
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840312"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1774)
                     /locus_tag="VFG22_09890"
     CDS             complement(<1..1774)
                     /locus_tag="VFG22_09890"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HET6416593.1"
                     /translation="MDFFEEQALARKRTLRLTLLFAAAVVGVIFCVYVLAMLFYGFGA
                     TDAAGVAYVVGDYDRVRPAILALWNPRVFLFAMSATVTVVGLGSLYKIAQLRGGGSTV
                     AQSLGGRRVDPDSTRLEERRLLNVVEEMAIASGIPVPEVFVLDREPGINAFAAGNTTS
                     DSVIGVTNGTLQLLRRDELQGVVAHEVSHILNGDSRINVRAIGLLHGIFLLALIGRFL
                     IRGSTYNRKKEGAGVVLLGLGLLSIGSIGVFFGRIIQSSISRQRELLADASAVQFTRD
                     TEGLVGALKKIGGLGSSSYLETPRADEASHIFFSDAIRRLRLFTGLFRTHPPLGDRIR
                     KLEPQWDGEFEDVEFPQISDGMSTPPDARLAPVSAFAEAPTEESVSRSIEHIGSPRPE
                     QITFARSLHGALPELWIHAVRQAPMAQAVVFGLLLTQDEKLRGTELMRLAELTDSEMA
                     DLTLRFHSEALHRSSAEKIALVDMAMPTLRTLSFEEYERFRTVIDALMQSDQRIDLFE
                     YTLSRMIRRHLGRYFEGAGPTSLRFRSLRALLPDARVLLATLARVGSRSDAAAERAFR
                     HGAQTLQLKASAGAILPREQCSLAA"
     gene            complement(1778..2365)
                     /locus_tag="VFG22_09895"
     CDS             complement(1778..2365)
                     /locus_tag="VFG22_09895"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HET6416594.1"
                     /translation="MGWLLGFLLVAVALLAWFGGMYNSLVSLRNRFKNAFAQIDVQLK
                     RRHDLIPNLVETAKGYLTHERATLEAVISARNSAEGARSNAAADPGNAQAMQKLSTAE
                     AGLSGVLGRFFALSEAYPDLKSNQNMMQLSEELSTTENKISFARQAYNDAVMQYNNKC
                     EMVPSNIVAGMFGFQRAEFFEVESEDERGPVKVQF"
     gene            2435..3352
                     /locus_tag="VFG22_09900"
     CDS             2435..3352
                     /locus_tag="VFG22_09900"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6416595.1"
                     /translation="MEPEQTVLITGASTGIGLAIARALSGTRYRVILTARQSSLPRFA
                     LHSIEESERVRIRSLDVTNSDERTAIIAEAERDWRGVDVLINNAGVSYRAVVEHVEEQ
                     DRLAQMDINFRSPMELIRLVLPGMRERCRGKIITVSSVGGMMAMPTMALYSASKFALE
                     GAHEALWYEVRPWNIRVSLVQPGFVYSDGFQKVRYTPQSGRSEHELAEPYHAHYVNMS
                     PFIERLMKASSATPESVAKVVLRTIRRKRPPLRVPATLDAMGFAFLRRILPRTTYHWL
                     LYKLLPNVDSWGPSPTRPSSLPPSRDDKR"
     gene            complement(3353..4177)
                     /locus_tag="VFG22_09905"
     CDS             complement(3353..4177)
                     /locus_tag="VFG22_09905"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6416596.1"
                     /translation="MSRFQDKTVVITGASSGIGESCARKFVAEGARVVVAARSAEPLQ
                     RLVNELGAEVAHAVPTDVSDFAACERLLAEAKAKFGSIDILVNNAAHNCRGPVENLPM
                     DQMLQMLHVNLRGPMVLTRLALPTMRSQDSGTVVNVASLAGRFPLEDEATYSTTKFGL
                     RIFSFALAEELRGTNVRVCVVSPGPVDTGFITDAGVIDSVPPLVFSQPMSTADEIADL
                     ILESAADGARERVKPALGGKLATLGYLVPSMRRLLIPIFERKGEREKKKYIARHSK"
     gene            complement(4174..>4452)
                     /locus_tag="VFG22_09910"
     CDS             complement(4174..>4452)
                     /locus_tag="VFG22_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416597.1"
                     /translation="LVLVADATKPRSLDHLLEAQTKGLGFTSSRPSILLINKTDETRN
                     FALSQAQLDAAKAFPWHLIQASAKSGDNVVQAFTKLAELMIETRKATG"
BASE COUNT          885 a         1359 c         1342 g          866 t
ORIGIN      
        1 ccgcagccag gctgcactgc tcccggggca aaatcgcccc cgccgatgcc ttgagctgca
       61 gagtctgcgc cccgtggcga aaggcgcgct cggccgccgc gtccgatcgg ctgcccaccc
      121 tcgccaacgt ggcgagcaag acccgcgcgt ccggcaagag cgcgcggagc gatcgaaacc
      181 gtagagacgt tggcccagcg ccttcgaagt acctgccgag atgacggcga atcatccggc
      241 tcaatgtgta ttcgaacaaa tcgatgcgtt ggtcactctg catcaaagca tcgatgaccg
      301 tgcggaagcg ctcgtactcc tcgaacgata aggttcgaag ggttggcatc gccatgtcga
      361 ccagcgcgat cttctcagcg gatgagcgat gtagagcctc cgaatgaaac cgaagggtga
      421 ggtcggccat ctccgagtcc gtgagctcgg ccaaacgcat cagctcggtt ccgcggagct
      481 tttcgtcctg cgtaagcagc agcccgaata cgaccgcctg tgccatcggt gcctggcgaa
      541 cggcgtgaat ccacagctcg ggcagggccc catgaagcga gcgggcgaat gtgatctgct
      601 ccggacgtgg gctgccgatg tgctcgattg atcggctcac cgattcctca gtcggagcct
      661 ccgcgaacgc gctgactggg gcgagacgcg catcgggtgg ggtgctcatg ccgtccgaga
      721 tctgtggaaa ctcgacgtcc tcgaactcac catcccattg cggctcgagc tttcggatgc
      781 ggtcgccgag cggcggatgc gtgcggaaca gcccggtgaa gagacgcagg cgacgaatgg
      841 cgtctgagaa gaagatgtgg cttgcctcat cggcccttgg tgtctcgagg tacgagctcg
      901 aacccaggcc gccgatcttc ttgagagcgc caacgagtcc ttccgtgtcg cgtgtgaatt
      961 gcacggcgga cgcatctgcg agcagctcgc gttgccgtga aatcgagctt tggatgatgc
     1021 gcccgaaaaa gacgccgatt gaaccaatcg acagaagccc gagtccgagc aagacgacgc
     1081 cggcgccttc cttctttcgg ttataggtcg atccacggat cagaaatcga cctatgagcg
     1141 cgagcaagaa gattccgtga aggagcccga tcgcacggac attgatacgg gagtctccat
     1201 tgaggatgtg actcacctcg tgtgcgacta cgccttgaag ctcgtctcgc cgcaagagtt
     1261 gaagcgttcc gttggtcact ccgattacgg aatcactggt cgtattgccc gcagcgaacg
     1321 cattgatgcc gggctctcga tcgagcacga aaacctcggg aaccggaatg cccgacgcga
     1381 tggccatctc ctcgacgacg ttgagcagac gtcgctcctc gagccgcgtc gagtcggggt
     1441 ccactcttcg tcctccgagg ctctgagcaa ccgtcgagcc tccgccgcgt agctgcgcga
     1501 tcttgtagag ggagcccagg cctaccacgg tgaccgtagc gctcatggcg aacagaaaca
     1561 cgcgggggtt ccacaaggcc aggattgcgg gacgcacgcg gtcgtagtcc ccgaccacgt
     1621 aggccactcc ggcggcgtcg gtcgctccga atccatagaa gagcatcgcg agcacataga
     1681 cacagaaaat gaccccgacg actgcagcag cgaagagaag cgtcaagcgc agcgtgcgct
     1741 tccgcgcgag agcttgctcc tcgaagaagt ccatagctca gaactgaact ttgacgggcc
     1801 cgcgctcgtc ttcggactcg acttcgaaaa actccgcgcg ttggaatcca aacatgccgg
     1861 caacgatgtt cgacggcacc atttcgcact tgttgttgta ttgcatcacg gcgtcgttgt
     1921 acgcctgcct tgcgaacgag atcttgtttt ccgtggtcga aagctcttcg gaaagctgca
     1981 tcatgttctg gttcgacttg aggtccggat aggcctcgga aagagcgaaa aatcggccga
     2041 gcacgccgct gagcccggct tctgcagtcg agagcttttg catggcctga gcgttgccgg
     2101 gatcggcagc agcgttgctc cgcgcgcctt cagccgagtt tctggctgag atgaccgctt
     2161 ccagggtcgc gcgttcgtgc gtcaggtagc ctttggccgt ttcgacgagg ttcggaatca
     2221 agtcatggcg ccgcttgagt tgcacatcga tctgagcgaa cgcgtttttg aagcgatttc
     2281 gcagactcac caggctgttg tacatgccac caaaccaagc aagcagcgcg acggcgacca
     2341 gcaggaaccc gagcaaccaa cccatagcaa cctccccaga acctccaaaa ggtactttca
     2401 gtgtaagggt caccccctat aaactcaact ccgcatggag cccgagcaaa cggtcttgat
     2461 tacgggcgcc agcacgggca tcgggctcgc catcgcgcgc gctctctcgg gaacccgcta
     2521 ccgcgtcatc ctgacggcgc gtcaaagctc gctgccgagg tttgcattac actcgattga
     2581 agaatcggag cgtgtccgca ttcggtctct cgacgtgacc aactcggacg agcgcactgc
     2641 gatcatcgca gaggccgagc gcgactggcg cggagtcgac gttctgatca acaatgccgg
     2701 cgtttcatat cgagcggtcg tggaacacgt cgaggagcag gatcgacttg cgcaaatgga
     2761 catcaacttc cgctctccga tggagctgat tcgactggtc ctaccgggga tgcgtgaacg
     2821 ttgccgcggc aagatcatca cggtgagctc ggttggcggc atgatggcga tgccgacgat
     2881 ggctctgtac agcgcttcga agttcgcgct cgagggggcg cacgaggcgc tctggtacga
     2941 ggttcgaccc tggaacatca gggtgagtct ggttcagccc ggcttcgtct actccgacgg
     3001 gtttcagaaa gtgcgttaca cccctcagag cggtcgctcg gagcatgagc tggcggaacc
     3061 ctaccacgcg cactacgtga atatgtcgcc gttcatagaa aggctgatga aggcgtcttc
     3121 cgccacgccc gaatccgtcg cgaaggttgt tctccgcacg atccgacgta agcgcccgcc
     3181 gcttcgtgtg cccgccactt tggacgcgat ggggtttgcc ttcttacgcc ggatcttgcc
     3241 gcggaccacc tatcattggc tgctctacaa gctgctcccg aacgtggatt cctggggacc
     3301 gtcgccgacg cgcccgtcgt cgctgccgcc ctcgcgagat gacaagcgat gatcacttcg
     3361 aatggcgggc gatgtacttc ttcttctctc gctcgccttt tcgttcgaat atcggaatga
     3421 gaagccgccg catggagggt actagatagc caagcgttgc gagcttgccg ccaagtgccg
     3481 gtttgactct ttctcgtgcg ccgtcggcag ccgactcgag tatcaggtcg gcaatttcgt
     3541 ctgccgtgct catcggctgc gagaagacga gcggcggaac ggagtcgatg acgcccgcat
     3601 ccgtgatgaa tccggtatcg accgggccgg gcgaaaccac gcaaacgcga acgttggtgc
     3661 cgcgaagctc ctcggccaga gcgaaagaaa aaatccgaag cccgaacttc gtggttgaat
     3721 aggtggcctc gtcttcgagc ggaaaacggc cggcgagcga tgcgacgttc accacggtgc
     3781 cagaatcctg cgagcgcatc gtcggcaacg cgagacgggt caagaccatg ggtccgcgca
     3841 agttcacgtg gagcatctgg agcatctgat ccattggaag attctcgacc ggaccgcgac
     3901 agttgtgcgc ggcgttgttt acgagaatgt cgatgctccc gaacttcgcc ttcgcttctg
     3961 ccagcaagcg ttcacacgcc gcgaagtccg acacatcggt gggtacggca tgggcgacct
     4021 cggcaccgag ctcgttgacc aaccgctgga gcggctccgc actccgcgca gccacgacga
     4081 ccctcgcacc ttctgcgacg aacttccgtg cacacgactc gccgatccca ctgctggccc
     4141 cagtaatcac cactgtcttg tcttgaaacc tactcatccc gttgcctttc tcgtttcgat
     4201 catcagctcg gcaagcttcg tgaatgcttg aacgacgttg tcccctgact tcgcacttgc
     4261 ttggatcagg tgccagggga aggccttggc ggcgtccagc tgggcttgac tgagagcgaa
     4321 gttccgtgtc tcgtcggtct tgttgatgag cagaatcgag ggccgcgagc tggtgaaccc
     4381 caaccccttg gtttgcgcct ccaacaagtg gtcgagcgat cgtggcttcg tggcatctgc
     4441 gacgagtacg ag
//
LOCUS       DASRZF010000223        24150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4619661, whole genome shotgun sequence.
ACCESSION   DASRZF010000223 DASRZF010000000
VERSION     DASRZF010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 24150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24150
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4619661"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..1203
                     /gene="gcvPB"
                     /locus_tag="VFG22_09915"
                     /pseudo
     CDS             58..1203
                     /gene="gcvPB"
                     /locus_tag="VFG22_09915"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
     gene            complement(1205..2281)
                     /gene="add"
                     /locus_tag="VFG22_09920"
     CDS             complement(1205..2281)
                     /gene="add"
                     /locus_tag="VFG22_09920"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HET6416598.1"
                     /translation="MELTLAQIQDLPKTDLHVHLDGSLRLSTMLELAEEQGVSLPGDD
                     PETLAKALHCGEHAGSLVEYLKAFDTTLKVLQTADALYRVAYELCEDAAAENVRYMEV
                     RYSPMLHTRGGLRLTSVVEAVLTGLWDAQKAHGIVANAILCGIRNISPESSFEMAQLA
                     VAYKNRGVVAFDLAGAEYDHPAKHHLRAFKLVRDNNIAVTIHAGEAYGPASIHQAIHV
                     CGAHRIGHACRLREDGDLLHYVNDHRIALECCPSSNVQTGAVKDLRTHPLKLYHDLGL
                     RVTINTDNRLITDTTVSRELWLCHTQMGLDRDDIHRMIINGFKAAFLPFHQKQTMVRS
                     VSRELSSAERASGPQGSRRADASA"
     gene            complement(2300..3193)
                     /locus_tag="VFG22_09925"
     CDS             complement(2300..3193)
                     /locus_tag="VFG22_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416599.1"
                     /translation="MGSFPHLAYGTPRKLPKPDSLPGRVVVLDVAFAANAGGASYERT
                     TLPFIKGLGARLAMWIDHHDHDRHRDYADDSRFILRTKAQHGACPELVTPERVAAAGP
                     IDTVCCHLDFDGLCSAAKWIRGGVEPYRGADADARAIDTRLGEPSERARVLDRALRAQ
                     PRNEALRGLMVRYLADGANDAALYREFQNAAEALQAMEREAKRLSARYEVHGKLAICD
                     ATRRDGPYDKTELLLIGQRLAPISLVHDETTVTVAAGFDSGIDLVSALGLNGGMPTRV
                     SVAAKRLDEVIEILGRFEVAE"
     gene            3361..4659
                     /gene="amt"
                     /locus_tag="VFG22_09930"
     CDS             3361..4659
                     /gene="amt"
                     /locus_tag="VFG22_09930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HET6416600.1"
                     /translation="MEEQLSELTTYANALWVLVSAALVFFMHAGFALVESGFCRRKNA
                     VMVLMKNFGVVAISSLVFYLVGYGVMFGEGNAFMGLASFFPANGGADADLPPYIFLFF
                     QLVFAATACTIVSGAIAERGRLLTFFLFNAIVTAIIYPVVGHWVWGGGWLSEQGFIDF
                     AGSTVVHGVGGGMALAGALIIGPRLGKFQPDGSVKPMPAHNFPLAALGVLILWLGWFG
                     FNGGSELAIDADVGRIVLVTNLAASAGFVAALAWIRMRSGLLDLSMALNGALAGLVGI
                     TAGCYNITGGWSIIVGLAAGILCVEGVLFIDKIKIDDPVGAITVHGICGMFGTLAVGL
                     VGYGNAATGEEPIGLLVGGGASQLGTQAMGTFSGVGFAFVVASIIWLAMKYLVPGGIR
                     VSEAHELEGLDIAECGVEAYNEELKGFHSGATAMSGAPAE"
     gene            4784..6133
                     /locus_tag="VFG22_09935"
     CDS             4784..6133
                     /locus_tag="VFG22_09935"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET6416601.1"
                     /translation="MRRILSLAVMCSLAFPAMALAEEPEAPKEAADDLAAEYGEEAAE
                     WTEKSPPKEAEMKMPEPPKMAMDDLEAQGEPEEDGSWKDGLSWQILASAFYRLGGYAG
                     DGVLAGSYNNLLDAEGRQGYPYTNYNGFGLNFAGGDVMYTGEKFAVRLDLRFGTGAPL
                     LTPAAPVKQAYAAWMPTEKLNFDLGFFDTIFGAEVVDEWNNANYTRGALYFLRQPFNH
                     MGLRMGLELTEIVGMTLMVTNGGVYGGTVIDDNETPALSWQLGFTPDGHNSVRRRGLR
                     DGYRGVGLFVGGSYAASGNDGNQDWVQFYDVVFSMDLKWAHIIFNGDYYINGSETSVD
                     TGATGTEFTYGHSLALIFDASDHWSIGLRGEHLSGNAYYRDETGPDFGGLATGTITLR
                     YKPVEYLVISMEGRGEWSTSDIYFSRSATDTDGDGILVPNQKKNYAAILGVTAHIGN"
     gene            complement(6230..6898)
                     /locus_tag="VFG22_09940"
     CDS             complement(6230..6898)
                     /locus_tag="VFG22_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416602.1"
                     /translation="MTRSLLLMGCTVLLGVAACKTATSTQISQSFRNPGFEETVFKQL
                     FVIGVAEDQEGRKAFEDAFVAAIVKKGGGAQASSNILPESTLLSEEQIQSAIEVSGCD
                     GVLITRLLSVENKTTNYTPPRKYNRPRTHLGFVHGGPAWGAGMGGFYGLYVTSFTEVH
                     RPGYFDTSKTFRLETNLYAVGASKDLVWTAQSETLDPKSVPDLLASMTKAVAKRLSKE
                     GLIP"
     gene            7055..7573
                     /locus_tag="VFG22_09945"
     CDS             7055..7573
                     /locus_tag="VFG22_09945"
                     /inference="COORDINATES: protein motif:HMM:NF015317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqhA family protein"
                     /protein_id="HET6416603.1"
                     /translation="MKRFLVACRFFLVLPVVGCVVLTAGVVTMGVGRIVTEGSQTFRT
                     GDFSAKAAKAVALASIEIIELFLVGTVAYIAAVGIYKLFLDDGKVELPFRLKIRSLKD
                     LKDKIIGLIVAALAVVFLGEAAEGAAGGVELLHYGGGIALVVASLALFMSQDGKAESA
                     GEAASGSPTSRP"
     gene            complement(7580..8611)
                     /locus_tag="VFG22_09950"
     CDS             complement(7580..8611)
                     /locus_tag="VFG22_09950"
                     /inference="COORDINATES: protein motif:HMM:NF015226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HET6416604.1"
                     /translation="MTGSSSDAAERSSFAEIADSTLRGIGQVVLQNNPWTGLVILVGI
                     YINSWIYGTLCLFGALLATLTALVVGADRGAIRAGLHGFNGALVAIALDAYTSSNYTE
                     GTIPGWQLMLLMILAVILSVFVAMAIGKFTSLVGLPALTASFIVVSLVFVSGALAVPM
                     IESTPIEAPAFANPAEAIAPYTLATLLEGSGKGVGQIFLQDNYITGALILVGIAINSR
                     IGALMALMACVLSTAVAMGFGYPEENIHAGLYTYNGVLTAIALGGLFFVLSWRSFLFT
                     LLAVLLTFEVTAGLATILTRVGLPVYTLSFVLVCWVLMAAGQSLRTLRYVMPADATTP
                     EDVLSKFRD"
     gene            complement(8608..9378)
                     /gene="ureG"
                     /locus_tag="VFG22_09955"
     CDS             complement(8608..9378)
                     /gene="ureG"
                     /locus_tag="VFG22_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HET6416605.1"
                     /translation="MHDHDDAHDHDHHHDDHGHSHEHLEHPGLFHERAQRLDERDYTR
                     RAFTVGIGGPVGSGKTALCLELCRALRTQRSLAAVTNDIFTREDAEFLQRNEALEPGR
                     IRAVETGGCPHAAIREDITSNLLALEGLTREFHPEILFIESGGDNLAADFSRELADYT
                     IYVIDVAGGDKVPRKGGPGITQSDLLVINKVDLADAVGADLGVMERDAKKMRGTGPTV
                     FAAVKHGKGVDLVIDGILSAWRDATELEPEAARRRVSR"
     gene            complement(9407..10072)
                     /locus_tag="VFG22_09960"
     CDS             complement(9407..10072)
                     /locus_tag="VFG22_09960"
                     /inference="COORDINATES: protein motif:HMM:NF013858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HET6416606.1"
                     /translation="MSDELMIWQIADTAFPVGGFAHSGGLESACHAGEVKGPGHLREF
                     IEAAIVQARFGTLPFVKGAYETDEPVGDLDALCDAFLVNHVANRASRSLGRGMLYVAG
                     ASFGHAALEALAQEWQDSGAPQHYGVVFGAVSRLLELPLPSTLRLAMFIAVRDLMSSA
                     VRLNVLGPLRAQGLLFELQPSITEASDPDGVPSPYEAVQTAPVMDLLQNTHDGLYSRL
                     FQS"
     gene            complement(10124..11842)
                     /gene="ureC"
                     /locus_tag="VFG22_09965"
     CDS             complement(10124..11842)
                     /gene="ureC"
                     /locus_tag="VFG22_09965"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HET6416607.1"
                     /translation="MSHTITRENYAALYGPTTGDKVRLGDSSLILEVEKDYAVYGDEC
                     KFGGGKVIREGMGQATGVSEAAALDLVIMNALIVDYTGIYKADVGIKNGRISAIGKAG
                     NPDVMNGVTPNMIVSVSTEVIAGEGMILTAGCIDTHIHFLCPQQYDEAIASGSTTMVG
                     GGTGPATGTTATTCTPGSHHIRLMLQALDDLPLNFGLTGKGNTARPEGLYEMLYAGAC
                     GFKLHEDWGTTPAAIDNCLTVADEYDLAVTLHTDTLNESGFYADSMAAMKGRTIHSYH
                     SEGAGGGHAPDILQICGEPNVLPSSTNPTRPYTKNTIDEHLDMLMVCHHLDPDLPEDV
                     AFAESRIRGETIAAEDLLHDMGAISAFSSDSQAMGRIGEVITRTWQTADKMKQQFGAL
                     PEDSGSGNDNFRIRRYVAKYTINPAIMHGMSHEIGSIEVGKLADLVLWSPKLFGTKPQ
                     MVIKGGMIAWSQMGDPNASITTPQPSFMRPMFGGRGKTASSLSVAFVSQASLDNHVVD
                     GFGLNKKLMAVKNCRNIGKADMKLNDKTPNIKVDAETYAVTADGKPLTCDPIDVVPLA
                     RRYYLF"
     gene            complement(11907..12668)
                     /gene="ureB"
                     /locus_tag="VFG22_09970"
     CDS             complement(11907..12668)
                     /gene="ureB"
                     /locus_tag="VFG22_09970"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF009671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HET6416608.1"
                     /translation="MHLTPREFDKLRLHNAGFLAQKRLARGLRLNHPEAVALIATVLF
                     ELIRDGEKSVAELMDLGKTILGRRQVMPGVPSMVYSVQVEGTFPDGAKLVSVGDPIAN
                     EDGDLTLALYGSFLPVPDLKVFGKDKDKLPKPGETITAKGNIVLNDDRETVTIEVKNV
                     GDRPIQVGSHYHFVETNRELAFDRAAAYGMRLDIPSGTAVRFEPGEKKTVQLVAIGGN
                     RVITGGNNAASGEVNDHNKEKALERLRDMGFRMSP"
     gene            complement(12731..13618)
                     /locus_tag="VFG22_09975"
     CDS             complement(12731..13618)
                     /locus_tag="VFG22_09975"
                     /inference="COORDINATES: protein motif:HMM:NF013899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HET6416609.1"
                     /translation="MDEVVPPADSFRPWAAPASPSYIATDGLLEVTWSRGKSAVTRAR
                     AASPLKLLVPRSKSEVARVSIATYGGGMLAGDETSLRVHLRPGAKAIISTQSSTKVFK
                     SVDGRTTRQSLTASLEPGAVAAFAPDPVACFADARYAQRQRFEVAGDATLFVLDWFTS
                     GRRARGEIWAFEHYSSRQQVFLDGRRIIEDAIRLDRSEGEIGSRFRGGRYHCHALLML
                     VGRRTQAAIERLRELASQQVTHGPKARAGLLVVANPVTHGVLLRVAGEETEAVAQFIS
                     REIEPCLGLFDASIWGRKF"
     gene            13848..14768
                     /locus_tag="VFG22_09980"
     CDS             13848..14768
                     /locus_tag="VFG22_09980"
                     /inference="COORDINATES: protein motif:HMM:NF013246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005254 - chloride channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HET6416610.1"
                     /translation="MLLEGRHSLARLLRTTVKIVIPLTVLSYAVLWAYRSTSLGTGSL
                     LYNKIGYVLSAISIFIAFRVNQAYGRWWEARQLWGSLINESRSWGRLVVTHFSSRAPL
                     SRDLLMRQIAYVNILRIALRHGTAEKGRAMMKAQLRRLLPEEDDAIVDRSKNVPTQLL
                     VSQAATIEEALSSGLESRLVWSQLTGILSTINAAQGGCERIKNTVFPAGITHFTRFMV
                     WAMAPLMTFAILGDSDRYDAIELPVVILVTSIFVVIEQLARELQTPFENAPDDTPMNA
                     ICVTIETDLRQMLGDGDLPAQEQPVRGVLM"
     gene            14834..15487
                     /locus_tag="VFG22_09985"
     CDS             14834..15487
                     /locus_tag="VFG22_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416611.1"
                     /translation="MTHASLFASLILAAITVGSLHSLAPDHWLPIAAVARARDWSRGR
                     AARVAFLCGFGHVTVSVMLGLLALLFGEQVLRSVGERMVSLAGLLLIGFGVVYALWGM
                     RSAMAHRFHGHHHHHYDHVHDPSNASTWSLFLIYCADPCIAVVPILFAAAPLSTVEQI
                     SIVVAYEVATVSAMVVLVALAHSGARRFKGPWIERYGDSTAGALIVATGIMVAVLDW"
     gene            16030..17376
                     /locus_tag="VFG22_09990"
     CDS             16030..17376
                     /locus_tag="VFG22_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416612.1"
                     /translation="MIRAQRLALVAMALVPWLSASHADAQGATDSDEHFHYLHFDIEG
                     ADAELQYPDGPPVEGLEDSNADGGPSSTDAEPETETTDSEDAELEIADVASFDLSGWL
                     WFNYSIQPWRDTTLAKYGNFEFNQLQLNGSAKWGGFSMRFEIRFFTFMFILRQGWIQY
                     EWKDNAIQLGHLNVPWGRHLFSSNSWWFTLGWYATVEDDYDYGLRYIKDTERYRVDVG
                     YYFRDQIGFSDPANRWDSDVTPLGEQQNREVSSAAVRGIYKLPHREGLRSEFGAVVQA
                     GLLPNVATGDLGYRWLAQVMYNGNYKGWQPVVQATRHEFRPNNPAEIDGVPVDDRVIR
                     ISQFGGYRDVAAKYWLCNFNLGKEIRFDRRFLDALVPYVEYSMFLKDEAAFPDSHLIT
                     VGMQVHLGPIWIWLDLLTGKNATYLNDSLANSALANGGNRSYRWENQTNLQIQYYF"
     gene            17419..18756
                     /locus_tag="VFG22_09995"
     CDS             17419..18756
                     /locus_tag="VFG22_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416613.1"
                     /translation="MNVHRKIPPSTRTSLFFILALAALGLAACTKPTPPADAAPTHAP
                     ETPEAPTEAPAAKEAPAFVCDDDIDTSELTPEQQALADKLWDQTLTYLRGFTTALETS
                     KLCLDSPLAMIQTNDGKSDDPQARCVSRYRDVELMVKHVNWVLDNPALARQCFDVQKN
                     YEAFPLYTPTEAMKQASDVAAWIDRPTLDEYHADKEGKVGEAGRDLVQNFEAILKNTN
                     STAFVDVDMTYQGLPDLWTAVGWLPFYAENPKAINERFRGGYAYAEVMGPWGLLRIKE
                     IEGELVGAEIGMTIQVESFYPYHFHHSQELYMNIGKSSCPNQNTFMVMHWDNPSFTQA
                     RTDEGWDVAIDKGKGKHRKATWFRPTSPSDPNEWIAYYERNAIHATKVGGKCGEGNAP
                     SGFAQVWARTSSRDNNQTTKICTPVGKDGNPLEGVTKVTETDPVVCNLEDWKP"
     gene            18822..19460
                     /locus_tag="VFG22_10000"
     CDS             18822..19460
                     /locus_tag="VFG22_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416614.1"
                     /translation="MNGRLPLIASLLLSIVLMGGYGCKKNESKTTVAGSWRSPELTDA
                     PFEKFFVIGAGSNDDYRRLYEDSLVAALERKGVEARASHDVLPQSEELTEEQIRSAMG
                     AGAYDAIIITSLLHVSQTVEVVEPRTERVPVGQVPGGVAWYQWAYDTVHVPGYARLHT
                     RYNIEARLYRVDDGERVWWGLSETVNPDSVMEIIGSVSAAMASQLEQDGLLR"
     gene            complement(19541..20164)
                     /locus_tag="VFG22_10005"
     CDS             complement(19541..20164)
                     /locus_tag="VFG22_10005"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HET6416615.1"
                     /translation="MRHCLLVVLVLASCLTLLGCGNATKIGEITVSYDEDADFSQFQT
                     FTVLTRELVPDAREPDEEEELFDELVNDLIIEAMTSEPVCMTFIPPEEVTEENEPDLF
                     AGNGLSRRTEEGTTWKCVGGWWWGRWGAFWDPCAWLVAVPIEFEVGSLLIPVGPRPEE
                     GEDPAPVFTGLGEAALQSQSNVERKVRIAVEEIFDQWPERRTCATSP"
     gene            20336..21919
                     /locus_tag="VFG22_10010"
     CDS             20336..21919
                     /locus_tag="VFG22_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416616.1"
                     /translation="MTQGREHGEGPRAFRCARLATLACIAIAALVSSTTRAEEPAEIS
                     QEALSKAQQELDEAQAKLNEAQAKLDAATKTLPTGMREPTAEEEDDPLDPVDDSTNEL
                     EPVGPGFMSADGEAKRLHWALNKDDSRYFQISLWLQVWTRALQLNPGTTIGDDPETSE
                     AENEQAWYGDVGIRRARVMMFGEIFPRVFLMMHFGINNQTFKRSNFKETFFFHDLWAE
                     FAAVREHLYIGAGLIYWNGISRMTNASTITAMSLDLPIVNWPTIEQTDQFARHLGLYA
                     KGKAGLFDYRVAVTRPFEPNVGPPTAMGNYNRYANTWAYAGYFQLQFWELESNVLPFT
                     TGTYLGTRRVMNLGTGFYLQPKGVAYLDAADELRERSLSVASADFFVDLPLAAKNGGA
                     LTAYGVYYWMDFGPNNIRNVGIMNPGDPGSGTSLNGPGNAYPMIGTGSTGYGQFGWLM
                     PWKINRLQFQPYVLSQMSKFEALNAMMFQFGVGMNMFVYGHNAKVSLEYRNRPIFDTE
                     GNVESRGGSGLVLQIHFFI"
     gene            complement(21912..23537)
                     /locus_tag="VFG22_10015"
     CDS             complement(21912..23537)
                     /locus_tag="VFG22_10015"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HET6416617.1"
                     /translation="MSASADDEYPLDPPDISSPEATINTFLTEATAAITAYGSGDEAA
                     MDASTDRLFQTLDVDMPNNDAEFRAAVESALYLFEILIRIDIPPAKQIPGPDLNPGSM
                     PAYWTIPHTELRLVRIDSTIGAPPAYRFSAETVGRLPEFYERARALPVKKRYANFDGV
                     KERYRLRPGFSAPAFVHRTVDSLPSGWFATLGGAPRWKWLAVVLVAVMGLMLFGLGYR
                     LARTLNGGNRSTSRLAALAHPLLAIGTIALIAFMRHVVIDWIRVIGREREVILGALSV
                     LAHLTVIWLLFLVARLFADAVIRMREMGAYALDAQLVRLVSKLVAVLLSLYALTSLAD
                     SLGVPVAPMLAGLGVGGLAVALAVRPTLENVVGGFVLFADAPVRVGEFCRFGDKLGTV
                     EAIGLRSVRVRGIDRTVITIPNADFSQLELVNFSRRDSILLQATLQLVYETTPDQLRL
                     VLVRLRELLLEHPKISPEPARVRFIRYGESSLDVEIFAYVMTRDFNEFLAIQEELNLR
                     IKDLVEDAGSGFAFPSQTLYVAQSDDPERSIPR"
     gene            complement(23684..>24150)
                     /locus_tag="VFG22_10020"
     CDS             complement(23684..>24150)
                     /locus_tag="VFG22_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416618.1"
                     /translation="FHDIPEVAEPSKRLKEILGLRADLDEFYLTNNPTDHSPREIGIQ
                     PRPLIGVMNYLSQSVEPPKRDIDAGRVTVTRNRDGSEFDWPVLSKDLFRVASSSKQPD
                     NAFVRVQYRDTWFYIEDSDLSSKSTFNLLDQLFQLQSGNTGNVAPMLTLPIN"
BASE COUNT         4663 a         7315 c         7503 g         4669 t
ORIGIN      
        1 ctgagccaac aaaacttcgc gatcgacacc ggcatgtacc cgctcggctc ctgcacgatg
       61 aagtacaacc ccaaggtcaa cgagtgggcc gcgaggttgt ccggctttgc agacctgcat
      121 ccgtacatgc cagacgagct cgtacaagga gccctggagc tcatgtggcg actcgagcgc
      181 gggcttgcgg aggtctgcgg catggatcgt gtttccctgc acccggccgc cggcgcccag
      241 ggcgagctca ccggcctcat gatgattcga gcgtaccatc aggccaaagg gcgcgacccc
      301 aggaaggtgc tgatccccga cacggcacac ggcaccaacc cggcctcctg cgcgctcaac
      361 ggactcgaag cggtcccttt ccccgttgga gaagaaggca tcgtgactac cgaggcgctc
      421 gcgcctttcg tcgacgatga cgtggcagcg gtgatggtca ccaatccaaa caccgtgggt
      481 ctgttcgaga gcaacatgcc cgccatagcg gagctcatcc acagcaaggg cggcctcgtc
      541 tatggcgacg gggccaacct gaacgcgttg atggggaagg cgcggccggg ggatctcgga
      601 atcgacgtga tgcagttcaa tctgcacaag accttcacga ccccgcacgg cggtggggga
      661 cccggttgcg gtcctgttgg ctacaaagcg cttctggatc cgtacgctcc cgcccccgtg
      721 gtcgagaagc gcgatggccg gttcgtcttg gactacgaga gccggccgca ttcggttgga
      781 cggctgcgtt cgtttcaagg caacttcggg atgatggtgc gcgcgtacgc atacctccga
      841 gagatgggag cgaaaggtct cgagcaagcc accgagctgg cggtgctcaa tgcgaactat
      901 ctacgagcac gtctcggcga ggtctggcac gtgccctacg atcagatctg tatgcacgag
      961 gtagtgatca gcgatcgtca cttgaaggac tcaggagtga cgaccatgga cgtcgcaaag
     1021 cgactgatgg actacggatt tcacccgccg acggtctact ttcccctcgt cgtgaagggc
     1081 gcgatgttga tcgagccgac ggagaccgaa agccttcaaa ctctggacga gttcgtcgac
     1141 gtcatgaaga ccatcagcga cgaagccatg atcgacccgg ggctcgcgaa agccgcaccc
     1201 aagatcacgc gcttgcgtcg gctcgacgag acccttgcgg cccggaagcc cgttctgcgc
     1261 tggagagctc ccgagacacg gagcgaacca tcgtctgctt ctggtgaaac ggcaaaaacg
     1321 cggccttgaa gccattgatg atcatgcggt gaatgtcgtc gcggtcgagg cccatctgag
     1381 tatggcaaag ccacagctcg cgcgagacgg tggtgtccgt gatgagccga ttgtcggtgt
     1441 tgatcgtcac gcgcagtccg aggtcgtgat agagcttgag cggatgggtt cgcagatcct
     1501 tgacggctcc ggtttgcacg ttggatgacg gacagcattc gagcgcaatc cggtggtcgt
     1561 tcacgtagtg aagcaggtcg ccgtcctcgc gtagccgaca cgcgtgtccg atccggtgtg
     1621 cgccacagac atgaatggcc tgatgaatcg aggcgggccc atatgcctca cccgcgtgga
     1681 tggtcaccgc gatgttgttg tcgcggacca gcttgaacgc cctgaggtgg tgcttcgcag
     1741 ggtgatcata ttcggctccc gcgagatcga aggcgactac gccccggttc ttgtaggcca
     1801 cggccagctg cgccatctcg aatgacgact cgggcgagat atttctgatt ccgcagagga
     1861 tggcgttagc gacgatcccg tgggcttttt gagcatccca gagtccggtg agcacggctt
     1921 caaccacgct cgtgagacgc aggcctccac gcgtgtgcag catgggcgag tagcggacct
     1981 ccatgtagcg cacattctcc gcggcggcgt cctcgcacaa ctcgtaggcg actcggtaga
     2041 gcgcgtcggc ggtctgtagc accttgagcg tggtgtcgaa ggccttcagg tactcgacca
     2101 gcgacccagc gtgctccccg cagtgaagcg ccttggccag cgtctccgga tcgtcgcctg
     2161 gaagagagac tccctgctct tcggcgagct cgagcatcgt cgacagtcgc aaagagccgt
     2221 cgaggtggac gtgcaggtcg gtcttcggga ggtcctggat ttgggcgagc gtgagctcca
     2281 tgaacttcag tgtacctcgt tattcggcca cttcgaaacg ccccaatatc tcaatgactt
     2341 catcgagtct cttggcggcg acgctcacgc gcgtcggcat cccgccgttg agtcccaggg
     2401 cgctcacgag gtcgatccca ctgtcgaagc cagccgccac tgtgacggtc gtttcgtcgt
     2461 ggaccagcga aatcggcgca aggcgctggc ctatgaggag cagctccgtc ttgtcgtacg
     2521 gaccgtctcg ccgcgtcgca tcgcaaatcg ccaacttgcc gtgaacctcg tatcgagcgg
     2581 acagtcgctt agcctctcgt tccatcgctt ggagtgcttc ggccgcgttc tggaattctc
     2641 ggtagagcgc cgcgtcgttc gcgccatccg cgaggtagcg gaccatcagg ccccgaaggg
     2701 cctcgttgcg aggctgagcg cgaagggccc gatcgagaac gcgcgctcgc tcactcggct
     2761 cgcccaggcg tgtgtcgatc gcccgcgcgt cggcgtcggc gcctcgataa ggttcgacac
     2821 ccccacggat ccatttcgcg gccgagcaga gcccatcgaa gtccaggtga cagcagaccg
     2881 tgtcgatcgg gccggcggcc gcaacccgct cgggcgtcac gagctcgggg caggcaccgt
     2941 gctgggcctt cgttcgcaat atgaaacgtg aatcatccgc gtaatccctg tgccggtcgt
     3001 ggtcgtgatg atcgatccac atggcgagac gcgcgccgag gcccttgatg aacgggagcg
     3061 tggtccgttc atagctggcc ccccctgcat tggccgcgaa ggccacgtcc aggaccacga
     3121 cacggccggg aaggctgtct ggcttgggca gcttgcgggg cgttccgtac gccaaatgcg
     3181 ggaaactacc catgatccga gcatgtggcc cccctgggtg cgacgcaact cgcggatacc
     3241 ctgatcggtt tttcccaaac cgatgcctcg gcgaaacaaa ccggaaatgc aaggcagcca
     3301 tagatcctcc cgagccgctg ccgagggtgc gctgcggcca tgactaagag gtgcaagatc
     3361 atggaagaac aactttcgga actgacaacg tatgcaaacg cattgtgggt tctcgttagc
     3421 gccgcactcg ttttcttcat gcacgcgggc tttgctctgg ttgagagtgg gttctgccgc
     3481 aggaaaaacg cggtgatggt gctgatgaag aacttcggtg tggtcgcgat ttcgtcgctc
     3541 gtattctact tggtaggcta cggcgtgatg tttggggaag gcaacgcctt catggggctc
     3601 gcgagcttct tccccgccaa tggaggagcg gatgcggacc tgccgcctta catattcctc
     3661 ttcttccagc tggtctttgc ggcgaccgcc tgcacgatcg tctcaggcgc aatcgccgag
     3721 cgtggccggc tcctgacctt cttcctgttc aacgcaatcg tgacggcgat catctatccg
     3781 gtggtcgggc attgggtctg gggcggaggc tggctctccg aacagggctt catcgacttc
     3841 gccgggagca cggtcgttca cggggtcggt ggcgggatgg cactggcagg tgcgctgatc
     3901 atcggacccc gactgggcaa gttccagcct gacggctcgg tcaagcccat gccggcacac
     3961 aatttcccgc tggccgcgct tggcgtgttg attctctggc tcggctggtt cggcttcaat
     4021 ggcggctccg agcttgccat cgacgccgat gtcggccgga tcgtgctggt gaccaacctg
     4081 gccgcgtccg ctggcttcgt cgccgcgctg gcatggatcc gaatgcgcag cggcttgctc
     4141 gatctctcga tggccctgaa cggtgcgctc gccggcctcg tgggcatcac ggcgggttgc
     4201 tacaacatca cgggcggctg gtcgatcatc gtcggcctcg cagccggcat cctttgcgtc
     4261 gagggcgtcc tgttcatcga caagatcaag atcgacgacc cggtcggcgc gatcaccgtc
     4321 cacggcattt gcggcatgtt cggaacgctc gccgtaggcc tggtcggcta cgggaatgca
     4381 gcgaccggcg aggagccgat cgggcttctg gtcggtggcg gagcatcgca gctcggcact
     4441 caggccatgg gcaccttctc gggcgtcggt ttcgcgttcg tcgtggcgtc catcatttgg
     4501 ttggccatga agtacctcgt gcctggaggc atccgagtct ccgaagcgca cgagctcgag
     4561 ggtctcgaca tcgccgagtg cggcgtcgaa gcctacaacg aggagctcaa gggctttcat
     4621 tctggcgcca ccgcgatgtc gggggcccct gctgagtagg agcttcgaga ggaggggtgg
     4681 gacggctccc cggtctgttt cgcccctctt ctctaccctt ttttcaatgt cggatttgga
     4741 taacaagttt gagcgtggag acacgctggg agtggaggta gtgatgagaa ggattctttc
     4801 cctggcggtg atgtgcagtt tggcgttccc cgcaatggcc ctcgcggaag agccggaggc
     4861 gccaaaggag gccgccgacg accttgcagc ggagtacggc gaagaagcgg cggaatggac
     4921 cgaaaagtct cctcccaaag aggccgagat gaagatgccc gagccaccca aaatggcgat
     4981 ggatgatctc gaggcacaag gggagcccga ggaggacggt tcctggaaag acggcctctc
     5041 gtggcagatc ctggcgagcg cgttctaccg actcggtggc tacgccggag acggcgtcct
     5101 ggcgggctcg tacaacaatc tgctcgatgc cgagggccgg cagggatacc cgtatacgaa
     5161 ctataacggc ttcggtctga acttcgccgg cggtgacgtg atgtacaccg gcgagaagtt
     5221 cgctgttcga ctcgacctgc gtttcggcac aggagccccc ctcttgacgc ccgccgctcc
     5281 ggtcaagcaa gcctatgcgg cgtggatgcc gacggagaag ctgaatttcg accttggctt
     5341 cttcgacacg atcttcggcg ctgaggtggt cgacgagtgg aacaacgcca actacacccg
     5401 cggcgccctg tacttcctcc gccagccgtt caaccacatg ggtcttcgca tgggcctcga
     5461 gctcaccgag atcgtcggga tgacgttgat ggtgaccaac ggcggcgttt acggcggtac
     5521 ggtgatcgac gacaacgaga cgcccgcgct ctcgtggcag ctcggtttca cgcctgatgg
     5581 ccacaacagc gtgcgacgtc gaggtcttcg cgacggttac cgcggggtcg gtctcttcgt
     5641 cggcggcagc tacgcggcaa gcggaaatga cggcaaccag gactgggtac aattttacga
     5701 cgtcgtattc agcatggacc tcaagtgggc ccacatcatt ttcaacgggg actactacat
     5761 caacggttcg gagaccagcg tagacactgg tgcaacgggc accgagttca cgtacggcca
     5821 cagcctcgcg ttgatcttcg atgcatccga ccattggtcg atcggtctcc gcggcgaaca
     5881 cctgagcggc aatgcctact acagggacga aacaggcccc gatttcgggg gcctcgccac
     5941 cggcacgatc acgcttcgct acaagccggt tgagtacctg gtgatcagca tggagggtcg
     6001 aggcgagtgg tcgacgagcg acatctactt ctcacgaagc gccactgata ccgacggtga
     6061 cggtatcctc gttccgaacc agaagaagaa ctacgcggcg attctgggtg tcacggcaca
     6121 catcgggaac tgacacgaac ggcgaccatc acgtgtgaaa ggccggctca ttcgagccgg
     6181 ccttttgccg ttaatgcttg gatggaatca cgtgggcacc gacgcgatcc tatggaatga
     6241 gtccctcttt gctgagcctc ttcgcgactg ccttcgtcat cgaggcaagg aggtcgggaa
     6301 cggatttcgg atcgagcgtt tccgattgcg ccgtccagac gagatccttg ctcgcgccca
     6361 ctgcgtatag gttggtctcc aatcgaaagg ttttgctggt gtcgaagtaa cccggccggt
     6421 gcacctcggt gaacgaggtc acgtagaggc catagaatcc gcccatgccc gcgccccatg
     6481 caggtccacc atggacgaag cccaagtgcg tcctcggccg gttgtacttc ctggggggtg
     6541 tgtagttcgt agtcttattc tcgacgctca gcagccgcgt gatcagaaca ccgtcgcagc
     6601 cgctgacctc gatcgcgctt tggatctgtt cttcagaaag gagcgtgctc tcgggcaaaa
     6661 tgttcgagct cgcttgggct ccgcctccct tcttcacgat cgctgccacg aacgcgtcct
     6721 cgaaggcttt tcgcccttct tggtcctccg caactccgat cacgaacagc tgcttgaaca
     6781 cggtctcttc gaagccaggg tttcggaagc tctgcgagat ctgggtactg gtagctgttt
     6841 tgcatgcagc cacccccaga aggacggtac agcccatgag gaggagcgat cgggtcatcg
     6901 acgggagagt agagcgaaac atgactgcgg gcatcagaaa gaacgggctg gaagccacaa
     6961 cgggctcccc aagtcggagt ttctccgaga aatcgtcgga ccggttggtt ttggatcgag
     7021 cccatgcgat cctcgtcgca aataggagtt gggcatgaag cgttttctcg ttgcttgtcg
     7081 tttctttctc gtcctcccgg tcgtcggctg cgtcgtcctg accgcaggcg tcgtcaccat
     7141 gggtgtgggc cgaatcgtga ccgagggttc gcaaacgttt cgtacgggcg atttctctgc
     7201 gaaagccgcg aaggccgtag cgctcgcaag cattgagatc atcgagctct tcctggtggg
     7261 aaccgtggcg tacatcgccg cggtcgggat ctacaagctc ttcctcgacg acggaaaggt
     7321 cgagctgccc tttcgcttga agattcgcag tctaaaagac ctcaaagaca agatcatcgg
     7381 cttgatcgtc gccgcactcg cggtggtttt tctcggtgaa gcggccgaag gcgcggccgg
     7441 tggggtggaa ctgctgcact atggaggcgg gatcgccctc gtcgtggcat cgctcgcatt
     7501 gttcatgagt caggacggca aagcggagtc tgcaggcgaa gcagcgtccg gatcgccgac
     7561 ctctcgtcca tgagcgtcgc tagtctcgaa acttgctcag cacgtcctct ggggtcgtcg
     7621 cgtcggccgg catgacgtac cgcagggttc ggagcgattg accggcggcc atcaagaccc
     7681 agcagaccag aacgaacgag agggtgtaca ccggcagccc aacccgcgtg aggatcgttg
     7741 ccagaccggc ggtcacctcg aacgtcaaga gcacggccag cagagtgaac aggaatgacc
     7801 tccaactcaa cacgaagaac agacctccga gggcgatcgc ggtgagcacg ccattatagg
     7861 tgtagaggcc ggcgtgaatg ttttcctccg ggtatccgaa gcccatggcg acggcggtcg
     7921 acaggacgca ggccatcagc gccatcagcg ctccgatgcg cgaattgatc gcgataccca
     7981 cgaggatcag ggctccggtg atgtagttgt cctgcagaaa gatttggcct acccccttgc
     8041 ccgagccctc gaggagggtc gccagcgtgt acggggcgat ggcttcggcc ggattcgcga
     8101 aggcgggagc ctcgatgggt gtgctttcga tcatggggac agcgagcgcg cccgagacga
     8161 agacaagact cacgacgatg aacgacgctg tgagcgccgg caatccgacg agactggtga
     8221 acttgccgat ggccattgcg acgaacaccg aaagaatgac cgccaagatc atgagcaaca
     8281 tcagttgcca cccggggatc gtgccctcgg tgtagttgct gctcgtgtac gcgtccaatg
     8341 cgatggcgac gagcgccccg ttgaatccgt gcaggcccgc acggatcgca ccgcgatccg
     8401 ccccgacgac gagcgccgtc agcgtggcga gcagagcgcc gaagaggcag agcgttccgt
     8461 aaatccatga gttgatgtag atccccacca agatcaccag gcccgtccag gggttgtttt
     8521 gcaagaccac ctgtccgatg ccccgaagcg tcgagtcggc aatctcggcg aacgacgagc
     8581 gctccgctgc gtccgatgac gagccggtca tcgtgacacc ctccgcctgg ctgcctcggg
     8641 ttccaactcc gtggcgtccc gccaggcgga caagatcccg tcgatgacga gatcgactcc
     8701 cttgccatgc ttgaccgccg cgaacacggt gggtccggtt ccccgcatct ttttcgcgtc
     8761 gcgttccatg acaccgaggt ccgcgcccac cgcatcggcg agatcgactt tgttgatcac
     8821 gagcagatcc gattgcgtga ttccagggcc gcctttgcgt gggactttgt cgccccccgc
     8881 aacgtcgatg acgtagatcg tgtagtcggc gagctcgcgg ctgaagtccg cagcgagatt
     8941 gtccccaccg gactcgatga acaggatctc cgggtgaaac tcccgggtaa gtccctcgag
     9001 ggcgagcaga ttgctcgtga tgtcctctcg aatggccgca tgcgggcagc cacctgtttc
     9061 gacagcgcga atccttccgg gctcgagggc ttcgttccgc tggagaaact ccgcgtcttc
     9121 ccgggtgaag atgtcgttgg tcacagccgc cagcgagcgc tgggtcctca gggcgcggca
     9181 gagctcgaga cacagggcgg tcttgccgct cccgacgggt ccaccgatgc caacggtgaa
     9241 ggcccggcgc gtgtagtccc tttcgtcgag ccgctgagca cgctcgtgaa aaagcccagg
     9301 gtgctcgagg tgctcgtgcg aatgaccatg atcgtcgtgg tggtggtcgt ggtcgtgagc
     9361 atcgtcgtgg tcgtgcatga gaggtctcct cgttgcgtat cgatcgtcag ctctggaaga
     9421 gccgggagta aaggccgtcg tgtgtgttct gtaagaggtc catcaccggc gccgtctgga
     9481 cggcctcgta cggcgacggc acgccatctg ggtctgatgc ctcagtgatc gagggctgga
     9541 gctcgaacaa gagaccctgc gcccgaagtg gaccgagcac gttgagcctc acagcgctag
     9601 acatcaggtc gcgcacggca atgaacatcg ccaacctcag ggtactcggg agaggcagct
     9661 cgaggagtcg gctcacggca ccgaacacga ccccgtaatg ctgcggggcg ccactgtcct
     9721 gccattcctg cgcgagcgct tcgagggcgg catgcccaaa cgacgctcca gccacataga
     9781 gcatcccacg acccagcgat cggctcgccc gattggccac gtgattcacc aggaacgcat
     9841 cgcagagagc gtccaggtcg ccaaccggtt cgtccgtttc gtatgctccc ttgacgaagg
     9901 gcaacgttcc aaacctcgcc tgaacgatcg ccgcctcgat gaactctcgc aaatgccccg
     9961 ggcccttcac ctcaccggcg tggcaggcag actcgagccc gcccgaatga gcgaacccgc
    10021 cgaccgggaa tgccgtatcg gcgatctgcc agatcatcag ctcgtcggac atcgaaacct
    10081 ttcgattcgc tcgttcgcga cctttagcaa cgcacgccca atctcaaaac aggtagtacc
    10141 gtcgagcgag cgggacgaca tcgatcgggt cgcaggtcag cggcttaccg tccgcggtca
    10201 ccgcataggt ttcggcgtcg accttgatgt tcggcgtctt gtcgttgagc ttcatgtccg
    10261 ccttgccgat gttcctgcag ttcttcacgg ccatgagctt cttgttgagc ccgaaaccgt
    10321 cgacgacatg attgtcgaga gatgcctggc tcacgaaagc cactgaaagg ctgctcgccg
    10381 tcttgcctcg gcctccgaac atcggacgca tgaagctcgg ttggggggtc gtgatcgatg
    10441 cgtttggatc acccatctgg gaccaggcga tcatgccgcc cttgatgacc atttgcggct
    10501 tcgtcccgaa gagcttgggg ctccacaaga cgaggtccgc gagcttgcct acctcgatgg
    10561 acccgatctc gtgcgacatc ccgtgcatga tcgcggggtt gatcgtgtac ttggcaacat
    10621 atcgccggat tcgaaagttg tcgttgcccg atcccgaatc ctcggggagc gcgccgaact
    10681 gttgtttcat tttgtcggca gtttgccagg ttcgcgtgat gacctcgccg atgcggccca
    10741 tggcctgtga atcggagctg aacgcgctga tcgcccccat gtcgtggagg agatcctccg
    10801 ccgcaatggt ttctccgcga atgcggctct ctgcgaacgc aacatcctca ggcaggtccg
    10861 gatccagatg gtgacagacc atcagcatgt cgaggtgctc gtcgatcgtg ttcttcgtgt
    10921 acggccgcgt cgggttcgtc gagctcggga ggacgtttgg ctctccgcag atctggagga
    10981 tgtcgggagc gtgcccgccg cctgcaccct cggagtgata ggagtggatc gtccggccct
    11041 tcatcgccgc catggagtcg gcgtagaacc ccgactcgtt gagcgtgtcg gtgtgaagtg
    11101 tcacggccag atcgtactcg tcggccacgg tcagacagtt gtcgatcgcc gcgggcgtcg
    11161 tgccccagtc ctcgtggagc ttgaaaccgc atgcgcccgc atagagcatc tcgtacagcc
    11221 cctcgggtcg agccgtgttg cccttccccg tcaagccgaa attgaggggc aggtcgtcca
    11281 gcgcctgaag catcagccgg atgtgatggc ttccgggcgt gcaggtcgtg gcggtcgtgc
    11341 cggtggccgg gccggtgccc ccgccgacca tggtcgtcga gccactcgca attgcttcgt
    11401 cgtactgctg cggacagagg aaatgaatgt gggtgtcgat gcaaccggcg gtgaggatca
    11461 tgccctcgcc ggcgatgacc tcggtgctca cgctgacgat catgttcggc gtcacaccgt
    11521 tcatgacgtc gggattgccg gccttgccga tcgcggaaat ccgcccgttc ttgatgccga
    11581 cgtcggcctt gtagataccg gtgtagtcga cgatgagcgc attcatgatg accaggtcga
    11641 gagccgcggc ctccgagaca cccgtggcct gtcccatgcc ctcacgaatg acctttccgc
    11701 caccgaactt gcactcgtca ccatagacgg cgtagtcctt ctcgacttcg aggatcagag
    11761 acgaatcgcc gagcctcacc ttgtccccgg tggtcggccc gtagagggcc gcgtagtttt
    11821 cgcgtgtaat cgtatgactc atgacaagct gctcctcgtg tgttggctgt gtcgtagatc
    11881 gctgctgaac ctggcacgtt gcggagtcat ggcgacatcc tgaagcccat gtcgcgcaga
    11941 cgctcgaggg ccttttcttt gttgtggtcg ttgacctcac ccgaggcggc gttgttgcca
    12001 ccggtaatga cgcggtttcc gccgatggcg acgagttgca cggtcttctt ttcaccgggc
    12061 tcgaaacgaa ccgcagtgcc cgacggaatg tcgagccgca tgccgtaggc agcagcccga
    12121 tcgaaggcca gctcccgatt ggtctcgacg aagtgatagt gacttccgac ctgaatcgga
    12181 cggtcgccga cgttcttcac ctcgatcgtc accgtctctc ggtcgtcgtt gaggacgatg
    12241 ttacccttcg cggtgatggt ctcgccgggt ttcggcagct tgtccttgtc cttcccgaat
    12301 accttgaggt ctggcaccgg gagaaagctt ccatacaggg ccagggtcag gtcgccgtcc
    12361 tcattggcga tggggtcccc cacgctcacg agcttggcgc cgtcgggaaa cgtgccttcg
    12421 acctgaaccg agtagaccat ggatggaaca cctggcatga cctgacgccg ccccaggatg
    12481 gtcttgccga gatccatgag ctcggcgacg ctcttttcgc cgtcgcggat gagctcgaag
    12541 agcaccgtcg cgatgagcgc caccgcttcc ggatgattca gtcgcagccc gcgcgccagg
    12601 cgcttctgcg caagaaagcc cgcattgtgc aggcgtagtt tgtcgaattc tcgtggcgtc
    12661 agatgcatgg ttccctcgtt ggtttggaag ggtggatgta agccaatttc gtttgtaatc
    12721 gctactgcat ttagaacttc cggccccaaa tactcgcgtc gaacaggccg aggcacggtt
    12781 ctatttcgcg gctgatgaac tgggcgacgg cttccgtctc ttcgcccgct acccggagca
    12841 gcaccccgtg cgtaaccggg ttcgcgacga cgagcagacc ggcacgggcc ttcgggccat
    12901 gggtgacctg ttggctggcc aactcacgca agcgctcgat ggccgcttgt gtcctccggc
    12961 ctacgagcat gagaagcgcg tggcagtggt atcggcctcc acgaaagcgg ctgccgattt
    13021 ccccctcgct gcggtcgagc cgaatcgcgt cttcgatgat ccggcgccca tcgagaaaca
    13081 cctgctggcg cgagctgtag tgctcgaacg cccagatctc gccacgggcg cggcgaccgc
    13141 tcgtgaacca atcgagcacg aacaaggtgg cgtctcctgc gacctcgaat cgctggcgct
    13201 gcgcgtatcg tgcgtcggcg aagcaagcga cgggatcggg tgcgaaggca gcaacggctc
    13261 ctggctctag cgaggccgtg agcgactgtc gtgtggtgcg gccgtcgacg cttttgaaga
    13321 ccttggtcga agactgagtg gagatgatcg ccttggcgcc cggtcgaaga tggacgcgca
    13381 gactggtttc atcgccggcg agcatgccgc cgccgtaggt cgcgatcgaa acccgggcga
    13441 cctcgctctt gcttcgcggc acgaggagct tgagcggtga agcggcgcgc gcccgagtca
    13501 ccgcgctctt gcctcgagac caggtcactt cgagcaggcc gtccgttgcg atgtagctcg
    13561 gcgatgctgg cgcggcccac ggtctgaaag agtcagccgg cggtactacc tcgtccaacc
    13621 ccggatgatc cgcgcgcgcc gtttgtccca atgcaggagg cataccagga gcgtgatgtc
    13681 ccgcttttcg attcaacagt caaggtcgat cgcaatgcca gtcgcgcaaa ggttgcgcgg
    13741 tcgctcactc gtcaccctgt cgtcaccgaa acccagggtc ccactcgaca tgaacgcgac
    13801 gcttcagatt gatctcgtcc ctcagccggt gatagcttcg cgccgacatg cttctcgaag
    13861 gccgtcactc gctcgctcga cttcttcgca ccacggttaa gatcgtgatt cccctcacgg
    13921 tgctgtcgta cgcagttcta tgggcgtacc ggtcgacgag cctaggcacc ggctctctcc
    13981 tatacaacaa gatcgggtat gtcctgtcag ccatttcgat tttcatcgcc tttcgggtga
    14041 accaggccta cggccgatgg tgggaagccc ggcagctttg gggttcgctc atcaacgaga
    14101 gtcggtcatg gggccgactg gtggtgacgc acttctcgtc gcgggctccg ctcagccgcg
    14161 acttgctgat gcggcaaatc gcgtacgtga atattttgcg catcgctctt cggcatggga
    14221 ccgccgaaaa gggccgggcc atgatgaagg cacagctaag aaggctgctc cccgaagagg
    14281 atgacgcgat cgtggaccga tcgaagaacg tgcccacgca gctcttggta tcccaggcgg
    14341 caacgatcga ggaagccctc tcgtctggtc tcgagagtcg gctcgtatgg tcccagctga
    14401 ccggcatctt gagcaccatc aatgcggcgc agggcggatg cgagcgcatc aagaacaccg
    14461 tgttccccgc cgggatcaca cacttcacaa ggttcatggt ctgggccatg gcgcctctca
    14521 tgacgttcgc catcctgggg gattccgacc gctacgatgc gatagagctt ccggtggtga
    14581 tcctagtcac gtcgatcttt gtcgtcatcg aacaactcgc tcgcgagcta cagacaccat
    14641 tcgagaacgc gcccgacgac acgcccatga atgcgatctg cgtgacgatt gaaacggacc
    14701 tacggcagat gctgggtgac ggggacctgc cggcgcaaga gcagcctgtt cgtggagttc
    14761 tgatgtaaca cggaaactaa aatggacgca tcccatccca agagctcaac ggcatttctg
    14821 agattgacac accatgaccc atgcaagcct tttcgcatcc ctgatcctgg cggcaattac
    14881 cgtcggctcg ctccattctc tggcgcctga tcattggctc ccgatcgccg cggtcgcgag
    14941 agcgcgcgac tggtcgcgcg gtcgtgcggc gcgcgtggcg ttcctctgcg ggttcggtca
    15001 cgtgaccgtc tccgtgatgc tgggtctcct ggccctcctc ttcggcgagc aagttcttcg
    15061 gtcggtcggg gaacgcatgg tgtccttggc aggtctgctc ctcattggat ttggggtcgt
    15121 gtacgcgctt tggggcatgc gaagcgcgat ggcgcatcgc ttccatggac atcaccatca
    15181 tcactacgac cacgtgcacg atccatcgaa cgcctcgacc tggtctttgt tcctgatcta
    15241 ctgcgccgac ccctgcatcg cggtcgttcc gatcctattt gcggcggcgc ccttgtcgac
    15301 ggtcgaacag atttcgatcg tggtggcgta cgaggtcgcg acggtgagcg ccatggtcgt
    15361 cctcgtcgcg ctggcgcact ccggggcccg acgcttcaaa ggcccgtgga tagagcgcta
    15421 cggcgacagc accgcgggcg cgctgatcgt ggccacgggc atcatggttg cggttctgga
    15481 ttggtgactg gaccacgcct caccagttcc aggggtgctg atgtagcgcg tcgcggagcc
    15541 tctctaggtc atctgcatcc gaagaagcac tgacattcac agtttccgtc gatgcaatca
    15601 tagggtccat agtccgggtc cccgcacacg tcctcgcatt cgcggacgca ttcctgttgc
    15661 gtctcgcacg caccgccctc gcagacgacc atatcggtct cgtcgctgca gccgaaccac
    15721 agcatgctgc atacagtcag aagcatccat ggaagtactc ttcgcatcag cagccctccg
    15781 tttggaggac ggtataccaa ggcggtcgtc gtgcacaaac gagatctagc aatggcgatc
    15841 cgacgattgc tccgcgatga ctgaaagctg catcatgcat tcgacactgg tacgccgcag
    15901 gggtcatcga atcgatcgat ttcggtttca ggatggtaac aagaggaccc aaaggtcttg
    15961 cggtcgccgc gatgaagtgg gtatagtcgg cgggaaattc ggggtgaaat cggagtgaag
    16021 gaggcagtga tgatcagagc ccaaagattg gcgctcgtag cgatggctct ggtgccttgg
    16081 ttgtccgcct cgcatgccga tgctcaggga gcgaccgatt ccgacgagca cttccactac
    16141 ctccacttcg acatcgaggg agcggatgcg gagctccagt atccggatgg tccgccagtg
    16201 gaagggctcg aggacagcaa cgccgacggt gggccctcga gcaccgatgc cgagcctgaa
    16261 accgaaacca ccgattcgga agacgcggaa ctggaaatcg cggacgttgc ctcgttcgac
    16321 ttgtctggat ggctgtggtt caactactcg atccaaccct ggcgagacac gacgctcgcg
    16381 aaatacggga acttcgagtt caaccagctt cagctgaatg gttcggcgaa atggggcggc
    16441 ttctccatgc ggttcgagat tcggttcttc acgttcatgt tcattctccg ccagggatgg
    16501 attcagtacg aatggaaaga caacgcgatc cagcttggcc atttgaacgt cccgtggggc
    16561 cggcatctct tctcatccaa ctcttggtgg ttcacgctgg gatggtacgc gaccgtcgaa
    16621 gatgactacg actacggtct ccggtacatc aaagacacgg agcgataccg ggtcgacgtc
    16681 ggctactact tccgcgatca aatagggttc tccgaccccg ccaaccgatg ggattcggat
    16741 gtcacgccgc tcggggagca gcagaaccgt gaggtctcga gcgccgccgt acgcggcatt
    16801 tacaaactcc cgcaccgtga gggactcaga agcgaattcg gtgctgtggt ccaggcaggg
    16861 ttgcttccca acgtcgccac gggcgacctc gggtatcgat ggctcgcgca agtcatgtac
    16921 aacgggaact acaaaggttg gcagccagtc gttcaggcga cacggcacga gttccgcccg
    16981 aacaacccgg ccgagatcga cggcgtgcca gtcgacgacc gcgtgatccg catctcgcag
    17041 tttggcgggt atcgagatgt cgcggccaag tactggctct gcaacttcaa tctcggcaaa
    17101 gagatccgct tcgatcgcag gttcctcgac gcactcgttc cctatgtcga atacagcatg
    17161 ttcttgaaag acgaggcggc cttccccgac tcgcacctga tcacggtcgg catgcaagtt
    17221 cacttgggcc cgatctggat atggctcgat ctgctgacgg gcaagaacgc gacctacttg
    17281 aacgattccc tcgcgaacag cgcactcgcc aacggcggca accgctccta ccgatgggag
    17341 aatcagacca atcttcagat tcagtactac ttctgaccca ctagcccccc tcacccgcga
    17401 ccacctccgg agctctccat gaacgtccac cgaaagatcc caccgtcgac ccgcacgtct
    17461 ctcttcttca ttctagcttt ggccgcactc ggcctggccg cgtgtacgaa gccgactcct
    17521 ccagcggacg cggcgccgac gcacgccccg gagacccccg aagcaccgac cgaggctccc
    17581 gcggccaaag aggctcccgc cttcgtctgt gacgatgaca tcgatacgag cgagctgacg
    17641 ccggagcaac aggcgttggc cgacaagctc tgggatcaaa cgctcactta cctgcgaggg
    17701 ttcacgaccg cgctcgagac ctcgaagctc tgcttggatt ccccgttggc catgattcag
    17761 acgaacgacg gaaagtcgga cgatccgcaa gcgcggtgcg tttcgcgcta ccgcgacgtc
    17821 gagctcatgg tgaagcacgt caactgggtg ctcgacaatc ccgcgttggc ccggcagtgc
    17881 ttcgacgtcc agaagaacta cgaagcgttt ccgctgtaca cgccaaccga agcgatgaag
    17941 caagcttcgg atgttgcggc ttggatcgat cggccgaccc tcgatgagta ccacgccgac
    18001 aaggaaggaa aggtgggcga agccggacga gacctcgtcc agaatttcga ggcgatcctg
    18061 aagaacacca actcgacagc gttcgtcgac gtcgacatga cctaccaagg cctgccggat
    18121 ctctggactg cggtcggctg gcttccgttc tacgccgaga atccaaaagc gatcaacgaa
    18181 cgattccgcg gtggatacgc ctatgccgag gtgatgggcc cgtggggcct cctgcgtatc
    18241 aaggaaattg aaggagagct cgttggtgcc gagatcggaa tgacgattca agtcgaatcg
    18301 ttttatccct accacttcca ccactcgcaa gagctctaca tgaacatcgg caagtcctcg
    18361 tgtccgaacc agaacacctt catggtgatg cattgggaca acccgagctt cacccaggcg
    18421 cggacggacg agggttggga cgtcgccatc gacaagggca agggcaagca tcgcaaggct
    18481 acctggttcc gaccgacctc accgtcggac ccgaacgagt ggattgcgta ctacgagcgc
    18541 aatgccattc acgcgacgaa agtgggcggc aagtgcgggg agggcaacgc gccctccggc
    18601 ttcgcgcagg tctgggcgcg cacctcctcg agagacaaca atcagacgac caaaatctgc
    18661 accccggtgg gcaaagacgg caatccgctc gagggggtca cgaaggtgac cgagactgat
    18721 cccgtcgttt gcaacctcga agactggaag ccctgaagtc ggcgaaaagt cccgattccg
    18781 ttgcttgcgc gattcggcgt tcgcccctag cctcgcgagc catgaacgga cgactgcccc
    18841 tcatcgcatc actgcttctt tcgatcgtgc tcatgggggg ctacggctgc aagaagaacg
    18901 aatcaaagac cacggtcgca ggttcttggc gaagcccaga gctcacagac gctcctttcg
    18961 agaagttctt cgtcatcggg gcgggaagca acgatgacta tcgtcggctc tacgaggata
    19021 gcctcgtggc cgcgttggag aggaagggag tcgaggcgcg ggcgagccac gacgttcttc
    19081 cccaaagcga ggagctgacg gaggagcaga ttcgaagcgc gatgggcgct ggcgcctacg
    19141 acgcgatcat catcacgagc cttcttcacg tgagccagac tgtcgaggtc gtggaacctc
    19201 gaacggaacg tgtgccagtc gggcaggttc caggcggggt cgcctggtac caatgggcct
    19261 acgacaccgt ccacgtgccg ggctacgcga ggcttcacac acgctacaac atcgaggcca
    19321 ggctgtatcg cgtcgatgac ggagagcgcg tctggtgggg gctctcggaa accgtgaacc
    19381 ccgactcagt catggagatt atcggctcgg tcagcgccgc gatggcatcg caactagagc
    19441 aagacggact cttgcgctag cagaaggcag agctgccggg tcgcggagtt ctcatgtgag
    19501 tcacgcgcgc tcagcgtggt gacgggcatc ggggttgcgg ctacggactg gtagcgcagg
    19561 tgcgtcgttc gggccactga tcgaaaatct cttccacggc gatacgaacc ttgcgttcca
    19621 cgttggactg actctgcagg gccgcctccc cgagccccgt gaaaactggc gctgggtcct
    19681 cgccctcctc aggccggggt ccgacgggga tcagcaggct gccgacttcg aattcgatgg
    19741 ggaccgccac cagccaggca cagggatccc agaacgcgcc ccaccttccc caccaccatc
    19801 ctccaacgca tttccacgtc gtgccctctt ctgtcctccg cgagagacca ttccctgcga
    19861 acaggtccgg ctcattctcc tcggtgactt cttcgggggg aatgaaggtc atgcagaccg
    19921 gctcagaggt catcgcctcg atgatcaagt cgttgacgag ctcgtcgaag agctcctctt
    19981 cttcatctgg ctcacgtgca tccggcacca gctcacgcgt caggaccgtg aaggtctgga
    20041 actgggagaa gtcggcatcc tcgtcgtagc tgaccgtgat ctcgccgatt ttggtggcgt
    20101 tgccgcaacc gaggagggtc aagcaagaag cgaggaccag aaccacgagc aaacagtgac
    20161 gcatgatgaa ctccgtggag tcatcgctag cacggcggaa cgcagaatca attgcgcaac
    20221 catatgcctt ctgccggttg ataagattcg ccgaagccgt catagcccgt gagatgatac
    20281 tgtcgccgcg gcaatcccaa aggcaagagt ggtcgcgcac aaagaggcga gtccgatgac
    20341 gcaaggacgg gaacacggcg aggggccgcg agcgttccgg tgcgcccggc tcgcgaccct
    20401 ggcctgcatt gcaatcgcgg cactggtgtc gagtacgaca agggcggaag agccggcgga
    20461 aatctcgcag gaggcgctca gcaaggctca gcaggagctg gatgaagcac aggccaagct
    20521 caacgaagca caggccaagc tggatgccgc tacaaaaacg ctccccacag gcatgcgcga
    20581 accgacggcc gaggaggagg acgatcccct cgatccggtc gacgactcga ccaatgagct
    20641 cgaaccggtc ggtccgggat tcatgtcggc cgacggggag gcgaagaggt tgcactgggc
    20701 cttgaacaag gacgactcgc ggtacttcca gatttcgctc tggttgcagg tgtggacacg
    20761 tgcgctgcaa ttgaaccctg ggacgacgat cggagacgac cctgagacct ccgaagccga
    20821 gaacgagcag gcctggtacg gagacgtcgg gattcgccgc gctcgcgtga tgatgttcgg
    20881 cgagattttt ccgcgcgtgt ttttgatgat gcacttcggc atcaacaacc agacgttcaa
    20941 acgttcaaac ttcaaggaga ctttcttctt ccacgacctt tgggcggagt ttgccgcggt
    21001 gcgagagcat ctctacatcg gcgctggact catctattgg aacggcattt ctcgcatgac
    21061 caacgccagc acgatcactg cgatgagcct cgatctaccg atcgtgaact ggcctacgat
    21121 cgagcagacg gatcagttcg cccgtcattt agggctttac gccaaaggca aagccgggtt
    21181 gttcgactat cgcgtggcgg tgacgaggcc cttcgagccc aacgtggggc cacctacggc
    21241 catgggcaac tacaaccgat acgccaacac ctgggcctat gccgggtatt ttcaacttca
    21301 gttctgggag ctcgagtcca acgtgctccc cttcaccact ggcacttatc tcggcacgcg
    21361 gcgcgtcatg aatcttggca ccgggttcta cctgcaaccg aagggggtcg catacctcga
    21421 tgccgccgat gagctgcgcg agaggagtct gtcggtcgcc tcggcggact tcttcgtcga
    21481 cctgccactc gcggcgaaga acggcggcgc gctcaccgcc tacggggtgt actactggat
    21541 ggacttcgga ccgaacaaca tccggaacgt cggcatcatg aatccaggtg atcctgggtc
    21601 cgggacatcg ttgaatggac cgggcaacgc gtacccgatg atcgggacgg gaagcaccgg
    21661 ttacggccaa ttcggctggc tgatgccctg gaagatcaac cggcttcagt tccaaccgta
    21721 cgtcttgagc cagatgtcga agttcgaggc tctcaacgcc atgatgtttc agttcggtgt
    21781 tggcatgaac atgttcgtct acggacacaa tgccaaggtc agcctcgagt atcgaaaccg
    21841 gccgatcttc gacaccgagg gcaatgtgga gagccgtggg ggcagcggtt tggtcctcca
    21901 gatccacttt ttcatctagg gatggatcgc tcggggtcgt ctgactgcgc gacgtagagg
    21961 gtttgcgagg ggaaagcaaa gccgctgccc gcgtcctcga ccagatcttt gatgcgcaga
    22021 ttgagctcct cttgaatcgc caagaactcg ttgaaatccc gagtcatcac gtacgcgaag
    22081 atctctacgt cgagtgacga ttcgccgtag cggatgaatc gaacgcgcgc tggctcgggc
    22141 gaaatcttcg ggtgctcgag caagagctca cgcaatcgca cgagaacgag ccgcaattgg
    22201 tcgggcgtgg tctcgtagac gagctggagc gtggcctgca ggaggatcga atcccggcgc
    22261 gagaagttca cgagctcgag ctgggaaaag tcggcattcg gaatcgtgat gaccgtgcga
    22321 tcgattccgc gcacgcggac cgagcgcagg ccaatggctt cgaccgtccc gagcttgtcg
    22381 ccgaagcggc agaattcgcc gacgcggacc ggtgcgtccg cgaagagcac gaagccacct
    22441 accacgttct cgagagtggg ccgtacggcc agagccaccg ccaagccacc gacgccgagc
    22501 cctgcaagca tgggcgccac cggtactccg aggctgtctg ccaagctagt gagcgcgtag
    22561 agtgacagca gcacggcgac cagcttggaa accaggcgca cgagttgcgc atcgagggca
    22621 taggccccca tttcgcgcat ccgaatcacg gcgtcggcga acagcctcgc gaccaggaag
    22681 agcagccaaa tcacggtgag gtgtgcgagg acggacaagg ctcccaagat cacctcgcgc
    22741 tcgcgtccga tcacacggat ccagtcgatg accacgtgcc gcatgaaggc gatcaaggcg
    22801 atggtgccga tggccaagag ggggtgagcc aaggcagcca ggcgggaggt cgatcgattc
    22861 ccgccgttga gagttctggc caatctgtag ccgaggccga agagcatcag gcccatgacg
    22921 gctacgagga ccaccgcgag ccacttccac cgaggcgcgc cacccaaagt ggcgaaccag
    22981 ccggatggga gcgaatcgac agtgcgatgc acgaaagcgg gtgccgagaa cccgggtcgt
    23041 aagcgatatc tctccttgac cccgtcgaag ttcgcatagc gcttcttcac gggcagggct
    23101 cttgcccttt cgtagaactc gggcaggcgc ccgacggtct cggcagagaa gcgataggcc
    23161 ggaggtgccc cgatggtgga gtcgatgcgc accagacgaa gctcggtatg gggaatcgtc
    23221 cagtacgccg gcatgcttcc gggattcaga tcgggacccg gaatctgctt cgcgggcgga
    23281 atatcgatgc gaatcaagat ctcgaaaaga taaagcgcag actcgaccgc cgcgcgaaac
    23341 tcggcatcgt tgttcggcat gtcgacgtcg agagtctgaa acaggcggtc agtgctagcg
    23401 tccatcgccg cctcgtcgcc gctgccatac gccgtgatcg ctgccgtcgc ctcggtcaga
    23461 aacgtgttga tcgtcgcctc gggggacgag atgtcgggag gatcgagcgg atactcgtcg
    23521 tctgccgacg cagacatagg catcgccagc atcgtgagcg ccgcaacgat gaatatcaga
    23581 cgggatcctg atgcgagtcg cgtgaaaggc gagtcatagt ttcggacatg caggtcgatt
    23641 gggtgagctt acttgcaccc aagcgctgcg ccaagtcacg ccgttagttt attggcagcg
    23701 tcagcatggg cgcgacgttg cctgtgttcc cagattgaag ctggaagagc tggtccagca
    23761 gattgaacgt ggacttcgag ctgaggtcag agtcctcgat gtagaaccag gtgtcgcggt
    23821 attggacacg gacgaatgcg ttgtcgggct gtttcgagct cgaggcgaca cggaagagat
    23881 ccttgctcag cacgggccag tcaaactcgg acccgtcgcg atttcgggtc acggtcacgc
    23941 ggccggcgtc gatgtcccgc ttcggtggct cgacgctctg cgacaggtag ttcatcacgc
    24001 cgatgagcgg acggggttgg atgccgatct cccgtggcga atgatcggtc gggttgttgg
    24061 tcaagtagaa ctcgtcgaga tccgctcgta ggcccaggat ctccttgagg cgtttcgatg
    24121 gctcggccac ctccggtatg tcgtggaagc
//
LOCUS       DASRZF010000224         2863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2903060, whole genome shotgun sequence.
ACCESSION   DASRZF010000224 DASRZF010000000
VERSION     DASRZF010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2903060"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VFG22_10025"
     CDS             complement(<1..306)
                     /locus_tag="VFG22_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416619.1"
                     /translation="MIDGRRPWFGRWWAVVLLIALVVLATAMLTVKALGLRSLDARAE
                     PFEQPLDDFDWGWWNQALGRWVRAAGVDYDAVRSDEGDLRRFVATLGAVGPRSTPERF
                     "
     gene            359..712
                     /gene="arsC"
                     /locus_tag="VFG22_10030"
     CDS             359..712
                     /gene="arsC"
                     /locus_tag="VFG22_10030"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_258191237.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HET6416620.1"
                     /translation="MSGITIWHNPRCSKSRQTLQLIVDHGMRPKVVEYLKTPPSTEEL
                     SEALTKLGLEPRALIRKKEAAYEELGLSDEGLSREELLAAMTAHPVLIERPLVFRGKR
                     AVLGRPPENVLSLLG"
     gene            complement(709..1413)
                     /locus_tag="VFG22_10035"
     CDS             complement(709..1413)
                     /locus_tag="VFG22_10035"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET6416621.1"
                     /translation="MRPIIALFDIDGTLIRAGGAGRRAVELALGEVFAQLDVEFSLDS
                     VEFAGRTDPWIVRTALGRYGVNAEDALIDEVLQRYVAHLPRELELASAFEVLPGVLGL
                     LTELSQREDVVLGLGTGNTKPAAYAKLARGGLDAFFSFGGFGSDHSDRAELLRTGLNR
                     GLEKTGASRGEAKVVVIGDTPHDVAAAHAIGADCVAVSTGGYDRAALEGAGARVVVSD
                     LRDAEVRAALKRIETD"
     gene            1463..2155
                     /locus_tag="VFG22_10040"
     CDS             1463..2155
                     /locus_tag="VFG22_10040"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET6416622.1"
                     /translation="MDLQLLAKLPLWLAAFLLSLTCHEAAHALVGKLGGDSTAARQVT
                     LDPLPHIRREPFGTLVVPILSFVFQGGGWMIGWASAPYDPTWASRHPRRAAAMAAAGP
                     AANFTLAVLAAIAIRVGISTGYFELPGAGITLETLAVAPAGAARGLALFCSVLFSINL
                     VLGCFNLLPLPPLDGYAIVPLVLNERMRDKWFGLFSGGGVWMGLIIAWVVFDRLMPPV
                     FRTAIGWLYAGI"
     gene            2182..>2863
                     /locus_tag="VFG22_10045"
     CDS             2182..>2863
                     /locus_tag="VFG22_10045"
                     /inference="COORDINATES: protein motif:HMM:NF016611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6416623.1"
                     /translation="MHWIGVVLACTLWLTATTSFAEAANAHYQLAKTLRAEGRYEETL
                     AEIDLAVAERPTYAQGHLTRGSILRRMGRYQEALQAFKRAVQLEPEDGGAQGLTGAAL
                     IRLERPKEAVPYLKRAIELEPKNSNHWLNLGVAYRESGDNDAAIATYEKALEAIPNDP
                     QILNNLGVALRKARRYDEAIKALEKASASAPHDAEVARNLAIAYRGAKHYGQAVPIYI
                     RALELGRGG"
BASE COUNT          534 a          960 c          843 g          526 t
ORIGIN      
        1 aaagcgctca ggcgtcgacc ggggccccac cgcgcccagc gtggcaacga accggcgcag
       61 atctccctcg tcgctgcgga ccgcatcgta gtccacgcca gctgcgcgca cccatcggcc
      121 aagcgcttgg ttccaccagc cccaatcgaa gtcatccaga ggctgctcaa acggctccgc
      181 acgtgcatcg agcgaacgca aaccgagcgc cttcaccgtg agcatggctg ttgccagcac
      241 gaccagggcg atcaagagga cgaccgccca ccatcgtcca aaccaaggtc ttcgaccatc
      301 aatcacgtcg ggagtattgc acgacgctcc gattcgttgc atcccgctca tcaacgacat
      361 gtcgggcatc acgatttggc acaatcctcg ctgcagcaag tctcgtcaga ctttgcagct
      421 catcgtcgat catggaatgc gacccaaggt ggtcgagtac ctgaagacgc caccttcgac
      481 tgaagagctg agcgaggccc tcaccaagct tggactcgag cctcgtgccc tcatccgcaa
      541 gaaggaagcg gcgtacgaag agcttgggct gagtgatgaa ggcctgagcc gcgaggagct
      601 cctggccgcc atgaccgcac atcccgttct catcgagcgc cctctcgtgt ttcgaggaaa
      661 gcgtgcggtg ctgggacgcc cgccggagaa cgtcctctcg cttctcggtt agtcggtttc
      721 gatccttttc aacgcggcgc gaacttcggc atctcgaagg tcggacacca cgacccgggc
      781 cccggctcct tccaacgcag cgcggtcgta cccgccagtg ctcacggcca cgcagtcggc
      841 gccgatcgca tgagccgcag ccacgtcatg tggcgtgtcg ccaattacga cgaccttcgc
      901 ttcgccccga ctggcgccag tcttctcgag gccccggttc aaaccggtgc gtaggagctc
      961 agcgcgatca ctgtggtcgg aaccgaaacc gccgaacgag aagaaagcat cgagccctcc
     1021 gcgggcgagc ttcgcatacg ccgcgggctt ggtgttccct gtgcccaggc ccaggaccac
     1081 gtcctcacgc tgactcagct cggtcagcaa ccccagcacg ccgggcagga cctcgaacgc
     1141 gctcgcgagc tcgagctctc gcggcaaatg ggcgacgtaa cgctgtagca cctcgtcgat
     1201 cagcgcatct tcggcgttca ctccgtatcg accgagggcc gtgcgtacga tccacggatc
     1261 ggtgcgtccc gcaaactcga ctgagtccag gctgaactcc acatcgagct gcgcaaacac
     1321 ctcgcccagg gcgagctcga ctgcacgtcg acctgcgcct cctgctctga tgagtgttcc
     1381 gtcgatgtcg aaaagggcga tgatcggtcg catcgtgcga atgtagcagc cgcggggttt
     1441 catactaggc tgcgctttgt cgatggacct tcaacttcta gccaagcttc ccctctggct
     1501 tgcagcgttc ttgctgtccc tcacttgtca cgaggcagcg cacgccctcg tcggtaagct
     1561 tgggggcgac tcgaccgcgg cgaggcaagt cacgctcgac ccactgccgc acatccgcag
     1621 ggagccattc ggcaccctgg tcgttcccat tctctcgttc gtctttcagg gtggcggctg
     1681 gatgatcggc tgggctagcg cgccgtacga tccgacctgg gcaagtcgcc atccacggcg
     1741 tgcagcagca atggcggccg cgggtcctgc cgcaaatttc acgcttgcag tgctggccgc
     1801 gatcgccatc cgcgtcggaa tctcaacagg ctacttcgag ctaccaggcg ctgggatcac
     1861 tctcgaaacg ctcgcggtcg ctcccgcggg cgcggcccgg ggcttggcgc tgttctgcag
     1921 cgttctcttc tcgatcaatt tggtcctcgg gtgcttcaat cttctccccc ttccacctct
     1981 cgatggctac gcgatcgtcc ccttggttct caacgagcgt atgcgcgaca aatggttcgg
     2041 cctgttctcc ggaggaggcg tgtggatggg cctgatcatc gcctgggtgg tcttcgaccg
     2101 gttgatgccg ccagtattca gaaccgcgat cggatggctc tacgcgggca tctgacctgc
     2161 cgtccgtgct atcctgctga cgtgcattgg ataggggtgg ttctggcttg cacgctgtgg
     2221 ctcacggcaa cgacgtcgtt cgccgaggcg gccaatgcgc actaccaact cgcaaagacc
     2281 ttgcgcgcag aaggacgcta cgaagagacg ctcgccgaga tcgatctcgc cgtggcggag
     2341 cggccaacgt atgcccaagg acatctcacc cgaggctcga ttcttcgtcg aatgggtcgc
     2401 tatcaggaag cgctgcaggc gttcaagcgt gcggtccaac tcgagcctga agatgggggg
     2461 gcacaaggtc tcaccggcgc agctctcatc cgtctcgaac gtcccaaaga ggctgttccg
     2521 tacctgaagc gagcaatcga gctcgagccc aagaacagca accactggct taatctcggc
     2581 gtggcctacc gagaatccgg cgacaacgat gctgcgatcg caacctatga gaaggcgctc
     2641 gaggcgattc cgaacgaccc gcagatcctc aacaaccttg gcgtggcgct tcgcaaggcg
     2701 cgccgctacg acgaagcgat caaagcgctc gagaaagcga gcgcatccgc tccacacgat
     2761 gccgaggtcg ctcgcaactt ggccatcgcc taccgcggag cgaagcacta cgggcaggcg
     2821 gttccgatct acatccgagc gctcgagctc ggccgtggcg gtc
//
LOCUS       DASRZF010000225         7595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1873885, whole genome shotgun sequence.
ACCESSION   DASRZF010000225 DASRZF010000000
VERSION     DASRZF010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 7595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7595
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1873885"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..1542)
                     /locus_tag="VFG22_10050"
     CDS             complement(4..1542)
                     /locus_tag="VFG22_10050"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET6416624.1"
                     /translation="MSTSQPIPPRAALVGGKYRLNEVIGSGGVGTVYRATHLWTEREV
                     AVKVLDQSLPHFHQLRDAFLMEARATVQLRHPNVVEVLDMGEDEAGATYMVMELLDGP
                     TLRDVLAEQGRLPPQETATILLPIIDALDYAHELGIVHKDFKPENIILSVDAFDVMTP
                     KLLDFGVAQILRETRSRDFAAPDEVIVGTPQYMSPEQARDERDLIGPQTDVWGVGVVW
                     YECLTGRSPFDRDTPLEVLNAVCEAPIDFDGVPESQASFLKHALERSPRRRTQRLSEL
                     RAQIEQSGLVVPATTDRVARSSRPPASERPSYAHRTLQGVGPSAYRSSGSRPVRFDSE
                     PLSLPKRSNRKSVLGGLALTIAVGLAAWWTIDESRPLESRILESPTVEVTRPERSAEP
                     EPEAAEVVRPERNAEPEPKRVTAPTSEAPPPAATEEILEDVIEDVIEEEHSSAEQAAP
                     VEEALPDPQPRVAPAKAAPKPTTPRTPRTPRTPSVQRPVEEEKGPPASRPGYQKPPDL
                     VTKW"
     gene            1854..4178
                     /locus_tag="VFG22_10055"
     CDS             1854..4178
                     /locus_tag="VFG22_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416625.1"
                     /translation="MDTDYAVPTEVDRVRARVAKIVETDGGVEEVETWVRVFPLSSDE
                     PAEANTYHLPATFAVLPSASDIDREVVIELEAIATNGNEVLVTRRLRTGFVPGQARLV
                     RMLIHRSCAEISCPEGDSCGCPGASSCATPSCVDERVSPESLETIDDPALLPPDSEFP
                     IAPGDGGVPDSGVPDGGIECEPPLVLCNQECVNTATDLRYCGGCNNACPNGFVCEASV
                     CRDLGDCRAAEGVCTGFTYCDDSTGNCLRGCANDDQCVRNQELCDVAAHECVCTPDFE
                     RCAFDCVDTQSDPRFCGGCETFCEAGEVCEVGICLDPGDCRSNGFGCSGFTYCDETTG
                     DCLRGCEETAQCTGKNQVCDTSLHECVCDVDFHECDGVCVSSLDAASCGTSCTPCLFP
                     PDAIPICALGVCDFVCTDGFERCDDRCCPTSCPPGEVLFEQACASVHVRTVDDQGDVG
                     EYTSIALDAAGQPHIAYYGRNGKKLLYSVRPTGGPWSQEVAESPGDVGKYASLAIDSA
                     GNPQIAHYEEGGRDLLFGRREVAGTWTQEIVSEKDDVGEYASLAFDANGTAQIAFYDK
                     SNKDLMFATELAGGAWSTQTVYSDGGVGKFASLAFDPNGTAHIAFYDETNKDLMIATR
                     LAAGVWFPPQRVYGEGDVDVGKFASLAFDANGTAKIAFYDETNKDLMLATKPALGAWS
                     VQTVDSLGDVGEFASLAIDASGTTHVSYFDATGLDLKYAVQKPGGVWSSETIDSVGEV
                     GQYTSIAIDAEGHAHISYYDKTNTNLKYALVAGN"
     gene            4217..5398
                     /locus_tag="VFG22_10060"
     CDS             4217..5398
                     /locus_tag="VFG22_10060"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET6416626.1"
                     /translation="MKHSELDSTDVSTSAERFAGQSVRGSTLSYAEYRRSRFFPSLDG
                     IRCFCILAVLWHHSMAQVLPWQFFDRGFLGVDMFFILSGYLIVTLLLREKDRHGSISL
                     SAFYARRTLRIFPIYYLLVFGTGVVTLFLSEDSATRRATNEIFGYAVTYTMNFAPASE
                     SVYSHLWSLSIEEQFYLLWPLAEKRLARTAMAPLLLLLFGLVVLADTGAFHGHETDGL
                     PWRLLIRGPFANAIVWGVLLAHLLHDPAWYERIRRMLRWRWSSVLILGLLLSVLAIPG
                     QRHPWPALATHVLMTLFLAACLVRPRNPLAPILNFKPIAYVGVVSYGIYLYHMHVRHF
                     CVALMGSFGADSPWLTFVLMTAASIGVASLSYHFLELPILAHRSRFVRTEAGRPAANA
                     K"
     gene            complement(5438..6178)
                     /locus_tag="VFG22_10065"
     CDS             complement(5438..6178)
                     /locus_tag="VFG22_10065"
                     /inference="COORDINATES: protein motif:HMM:NF004858.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HET6416627.1"
                     /translation="MASSLVTLDIDRSVATITMDDGKRNALSPAMFDEIYAALERAER
                     EGAALVITGREGVLSAGFDLKVMKSGGVQAVKMLRAGYALTARLLSFPTPVVIASPGH
                     AYAMGAFMLLSGDYRFGVPGPYTYVANEVAIGLPMPRVACEVLRLRLNPAARERAVVL
                     SEEFSPEEALQVGFVDALIPAERLLDAARDKAAALLNLDAAAHALTKKRLRADTLTNI
                     RAALPLDLKDALLLGAKRAAKARLARRK"
     gene            6177..7073
                     /locus_tag="VFG22_10070"
     CDS             6177..7073
                     /locus_tag="VFG22_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416628.1"
                     /translation="MRAFIARGVLQTSPLLQDFDLPLYTSALDARAWCNGKVLISMRA
                     ISIVLAREYLADAPGWPTREELDPNNPGVFIEAWDQGAVDVGNGRSDSAWVHDDLRAT
                     ALALSFGEQRALLIMADTYSYFGSDIAVMLERVRPRLPLEWSEAPILVSATHNHHGPD
                     TAFSINDDWFSLMADELAAAAEDAVAAMQPATVSSAVGDHGYGVDDVRDPVIRNTLLN
                     VLAIDAAEDGEPIATLVQWTSHPESTLGWHPPAEAANLEEVCAIKGWTDGNCTAEGRY
                     LTADYPGVLRARLQASRGGEVL"
     gene            7098..>7595
                     /locus_tag="VFG22_10075"
     CDS             7098..>7595
                     /locus_tag="VFG22_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416629.1"
                     /translation="MGPGAAPTWYVDEAHPVGDGLTVPDGAVPLTECEDRAPYLCRSF
                     AKTESIGTELANAVRAAMEQSKPVTVTELSVDIESFYTRLTNIGFRVLIADGDIGWQP
                     PTLYNCMGKPFSDDSCVEDGGEIIDDPLLAFFDSRIARGDVIESQIARVDFGNVGMLF
                     MPGELP"
BASE COUNT         1356 a         2357 c         2392 g         1490 t
ORIGIN      
        1 ttctcaccat ttcgtcacga gatctggcgg tttctggtag cccgggcgtg acgcgggagg
       61 gcccttttct tcttcgaccg ggcgttgcac actcggtgtc ctcggtgtcc tcggtgtcct
      121 cggagtggtc ggctttgggg ctgctttggc cggcgcgacc cgcggctgcg gatctgggag
      181 cgcttcctca acaggcgcgg cctgctctgc agatgaatgc tcctcctcga tgacgtcctc
      241 gatgacgtcc tcgaggatct cctcggtggc cgctggcgga ggcgcttcgc tcgtcggcgc
      301 ggtcacccgc ttgggctcgg gctcggcatt gcgttcgggt cgaaccactt ccgcggcctc
      361 gggctcgggc tcggcactcc gttcaggccg ggtcacctcc acggtcggcg attccaggat
      421 acgcgactcg aggggacggc tctcgtcgat cgtccaccag gccgccagac ccacggcgat
      481 ggtcagagcg agcccaccga gcaccgactt tcgattggac cttttcggta aactcaacgg
      541 ctcggaatcg aatcgcaccg gtcgagagcc cgagctcctg taagccgagg gcccgacgcc
      601 ctggagcgtc cgatgggcgt agctcggtcg ctcgctagca ggcgggcggg atgatcgcgc
      661 gacccgatcg gtggttgctg gcacgaccag gccgctctgc tcaatttgag ccctcagctc
      721 ggaaagacgc tgagtacgcc gccgaggcga acgctcgagg gcgtgcttca ggaacgacgc
      781 ctggctttcg ggaaccccat cgaaatcgat gggcgcctcg cagaccgcgt tgagcacctc
      841 gagaggcgta tcccgatcaa aaggagaccg accggtcaaa cattcatacc agaccacgcc
      901 gaccccccag acgtcggtct gcggcccgat gaggtcgcgc tcgtcgcgcg cttgctctgg
      961 agacatgtac tgaggcgtcc cgacgatgac ctcgtcgggc gccgcaaaat cccgcgagcg
     1021 cgtctcacga aggatctggg cgacgccaaa gtctaggagc ttcggtgtca ttacgtcgaa
     1081 tgcatcgacg ctgaggatga tgttctcagg cttgaagtcc ttgtgcacga tgcctagctc
     1141 gtgcgcatag tccagcgcat cgatgatcgg aagcaggatc gtggcagtct cttgcggcgg
     1201 tagtcggccc tgctcggcca ggacgtcgcg aagcgtaggg ccatcgagca gctccatcac
     1261 catgtaggtc gctcccgcct cgtcttcgcc catgtcgagt acctcgacga cgttggggtg
     1321 ccggagctgg accgtggctc gcgcctccat caggaaggca tcccgcagct gatggaagtg
     1381 cggaaggctc tgatcgagaa ccttgactgc aacctcccgc tccgtccaca ggtgcgtggc
     1441 gcggtacacg gtgccgaccc caccggaccc tatgacctcg ttgaggcggt acttgcctcc
     1501 gaccaaggcg gctcgaggcg gtatgggctg cgaagtggac atgaaccaag gtcgaccaag
     1561 cggctgctgg gtggatcaca gcagcaaacc aagaaggggg ggacagataa tggtgagaga
     1621 tttccgggcc ctgcacaaga ggaagcgggg tgcctttctc tcgcttggcc gtcggcttgc
     1681 gagcgcgcgg agcgtcggta cccttcttgg tcttagtgtc cccccatttg ccagacgcag
     1741 tcgcgccaac ctgtcatcgg agagatgcga gtcgcctcgc gccgctgctc gtcttgtttt
     1801 cactctttgg ggttaattgt tccgagacca cggcgactga gctcgtggta ttgatggaca
     1861 ccgactacgc ggtaccgact gaagtcgacc gtgtccgagc ccgggtcgcc aagatcgtcg
     1921 agaccgatgg cggcgtagaa gaagtggaaa cctgggttcg cgtcttcccg ctctcgagcg
     1981 atgagcccgc ggaggccaac acgtaccatc tgccggcgac gtttgccgtg ttgccgtccg
     2041 cttcggacat cgaccgcgag gtcgtgatcg agctcgaggc cattgccacc aacggcaatg
     2101 aggttctcgt tacccgacgc ctgaggaccg gattcgtgcc gggccaagcg cgtctggtga
     2161 gaatgctgat ccaccgttcc tgcgctgaga tctcctgtcc ggaaggcgat agctgcggct
     2221 gcccgggggc gagctcgtgc gccacgccat cgtgcgtaga cgaacgcgtc agcccggaga
     2281 gtctcgaaac catcgatgac cccgcgcttc tgccgccgga ttcggagttt ccgatcgcgc
     2341 ctggagacgg aggtgtgccg gacagcggtg tgccggacgg cgggatcgaa tgcgagccgc
     2401 ccctggtgct ctgcaaccaa gaatgcgtga acacggccac tgaccttcgc tactgcggcg
     2461 gctgcaacaa tgcgtgcccc aacggattcg tctgcgaagc gtcggtgtgc agggatctcg
     2521 gcgattgtcg cgcggccgag ggcgtatgca caggcttcac atactgcgat gactcgacgg
     2581 gcaactgctt gcgcggctgc gcgaacgacg atcagtgcgt ccgaaaccaa gagctctgcg
     2641 acgtggcggc ccatgaatgc gtctgtacgc ccgactttga aaggtgtgcg ttcgactgcg
     2701 tggataccca gtcagatcca cgcttctgcg gcggttgtga gaccttctgc gaggcgggcg
     2761 aagtctgcga ggtcgggatc tgcctggatc cgggcgattg ccgcagcaac ggcttcggat
     2821 gcagcggctt cacgtactgc gatgaaacca cgggggattg cttgcgcggc tgtgaggaga
     2881 ccgcgcagtg caccggcaag aaccaggtct gcgacacgag cctgcatgaa tgcgtttgcg
     2941 acgtagactt ccacgaatgc gatggcgtgt gtgtctccag cctggacgcc gctagctgcg
     3001 ggacgtcctg taccccttgt ctttttccgc cggacgcaat tccgatctgt gctttgggcg
     3061 tttgtgattt cgtctgcacc gatgggttcg agcgatgtga cgatcgctgt tgcccaacga
     3121 gctgccctcc tggcgaagtg cttttcgagc aggcgtgcgc gagcgtgcac gttcggacag
     3181 tggatgacca gggcgacgtc ggcgagtaca cttcgatcgc gctcgacgcc gcggggcagc
     3241 cgcacatcgc gtactacggg cgaaatggga agaagctgct ttacagcgtt cggccgactg
     3301 gcggcccatg gtcgcaagag gtagccgaat cgccgggcga tgtcgggaag tacgcctcct
     3361 tggcgatcga ttcggctggg aacccgcaga tcgcccacta cgaagagggt ggtagagatc
     3421 ttctctttgg aaggcgcgaa gtcgcaggaa cgtggactca ggagatagtg tctgagaaag
     3481 acgacgttgg agaatacgcc tcgctggcct tcgacgcgaa tggcaccgcc cagatcgcct
     3541 tttacgacaa gagtaacaaa gacctgatgt tcgccacgga gctcgctggc ggagcttggt
     3601 cgacgcagac ggtttacagc gatggcggcg tggggaagtt cgcgtcgctg gccttcgacc
     3661 cgaatggcac cgcccacatc gccttttacg atgaaaccaa caaagacctg atgatcgcga
     3721 cgcgactcgc ggccggagtc tggttcccgc cccagcgcgt ttacggcgaa ggcgacgtcg
     3781 acgtcgggaa gttcgcatcg ctggcctttg acgcgaacgg cactgccaaa atcgccttct
     3841 acgatgaaac caacaaagac ctgatgctcg caaccaagcc cgctctcgga gcttggagcg
     3901 tgcagacggt cgatagcctg ggtgacgtcg gcgagtttgc gtccctagct atcgacgcgt
     3961 ccggtacgac tcacgtcagc tacttcgacg cgacggggct cgatctcaag tacgcggttc
     4021 agaagcctgg aggagtctgg tcgagcgaaa cgatagacag cgtcggcgag gtcggtcagt
     4081 acacctcgat tgcgatcgac gcggaaggcc acgcgcacat cagctactac gacaagacga
     4141 atacgaatct caagtacgcg cttgtcgcgg gcaattagta ctctcaaagc cccaagcacc
     4201 gatccacgtc gaaacgatga agcactccga gcttgacagt acggacgttt cgacatcagc
     4261 agagcgcttc gccggccaga gtgtccgtgg ttcgacgctc agctatgcgg agtaccgccg
     4321 ctcgcggttc ttcccatcac tggatgggat ccgctgcttc tgcattctcg ccgtgctctg
     4381 gcatcattcg atggctcagg tcctgccgtg gcagttcttc gatcgcggat ttctcggcgt
     4441 cgacatgttc ttcatcttga gcggatacct gatcgtgacg ctcctgttgc gcgagaagga
     4501 caggcacggt tccatttcgc tcagcgcgtt ctacgcgcga cgaacgctca gaatttttcc
     4561 catctattac ctcttggttt tcgggactgg cgtcgtcacc ctgtttcttt ccgaagattc
     4621 ggccacccgc cgagctacga atgaaatatt tggatacgcg gtcacgtaca cgatgaactt
     4681 cgcaccggcg tcggagagcg tctactcgca cttgtggtcg ctctccatcg aagagcagtt
     4741 ctatctgcta tggccgctag ccgaaaagcg actcgcccga accgccatgg cacctctgct
     4801 tctgctccta ttcggcctcg tagtccttgc tgacacgggg gcttttcacg gtcacgagac
     4861 ggacggcttg ccttggcgtc tgctgatccg gggacccttc gcgaatgcga tcgtctgggg
     4921 cgtgctgctc gcgcacctgc tgcacgatcc ggcctggtac gagcgcatca gacgcatgct
     4981 cagatggcgc tggtccagtg tcttgattct ggggcttctg ctctcggtgc tggcgattcc
     5041 ggggcaacga cacccttggc ccgcgttggc gacccacgtg ctgatgactc tatttctagc
     5101 cgcatgcctg gtgcgtccgc gaaacccgtt ggcgcccatc ttgaacttca aaccgatcgc
     5161 ctacgtcgga gtcgtcagct acggaatcta tttgtatcac atgcatgtgc gtcatttctg
     5221 cgtggctctg atgggctcgt ttggcgccga ctctccgtgg ctcacgttcg tgctgatgac
     5281 ggccgcttcg attggcgtgg catcgctgag ctatcacttc ctggagctgc cgattctggc
     5341 gcaccgttcg cgcttcgttc gtaccgaagc cggtcgtcct gctgcaaacg cgaagtgagg
     5401 gcgtgttgcg agtcggtagg gcaagccggc gaggaggtta tttcctccgc gccagccttg
     5461 cctttgcggc acgcttggca ccaagcagca acgcatcctt gaggtccaag ggaagcgctg
     5521 cgcgaatgtt cgtcagcgtg tcggcgcgca ggcgcttctt ggttaacgcg tgcgcggccg
     5581 catcgaggtt caagagcgcc gcagctttgt cgcgcgctgc atcgagcaac cgctccgccg
     5641 gtatcaacgc gtcgacgaag ccaacctgca gcgcctcctc cggcgaaaac tcttcggaca
     5701 agaccaccgc gcgctcgcga gccgctgggt tcaagcgcaa tcgaagcacc tcgcaagcca
     5761 cacgcggcat cggaagaccg atcgccactt cgttggcaac gtaggtgtag gggcccggaa
     5821 ctccgaaacg gtagtcgccg gatagcagca tgaacgctcc catcgcgtat gcatgacctg
     5881 gcgacgcgat gaccacgggc gtcggaaacg acaacagccg tgcggtcagc gcgtaacctg
     5941 cgcgcagcat cttgaccgcc tgcacgccgc cagacttcat caccttgagg tcgaagcccg
     6001 cggacagcac cccttcgcgc cccgtgatga ccaaggccgc gccctctcgc tccgcgcgct
     6061 ctagcgccgc gtagatctca tcgaacattg ccggagatag cgcgttgcgt ttgccatcgt
     6121 ccattgtgat cgtcgcaacc gagcgatcga tgtcgagtgt caccaacgag ctagccatgc
     6181 gcgcgtttat agcacgtggc gtgctgcaaa cctctccgct cttgcaagac ttcgacctgc
     6241 cgttgtacac ctcggctctg gatgcacggg cttggtgcaa cggtaaggtg ctgatttcaa
     6301 tgagagcaat atcgattgtc ttggcgcggg agtacttggc cgatgctcca ggctggccta
     6361 cgcgcgagga gctcgatccc aacaatccag gtgtgttcat cgaagcctgg gaccaaggag
     6421 cggtggacgt cggcaacggt cgctcggatt ccgcatgggt ccacgatgat ctgagagcga
     6481 ccgcactcgc gctttcgttt ggcgagcagc gcgcgctcct gatcatggcc gacacctatt
     6541 cgtacttcgg ctccgatatc gccgtgatgc tcgagcgagt gcgacctcgc ttgccgctcg
     6601 aatggtcgga ggcgccgatc ctcgtttcgg cgacgcacaa ccatcacggg ccggacacgg
     6661 ccttcagcat caacgacgac tggttctccc tcatggcgga tgagctcgcc gcggcagcgg
     6721 aagacgcggt tgcggcgatg cagccggcca ccgtctcgtc ggctgttggt gatcatggat
     6781 acggagtcga cgacgtacgg gatccggtta tccgcaacac gctgctcaac gtgctcgcca
     6841 tcgatgcagc ggaggacgga gagccgatcg cgacgctcgt ccagtggacc agccaccccg
     6901 agtcgacgct cggctggcac cctccggccg aggcggcgaa tctcgaggag gtttgcgcca
     6961 tcaagggctg gacggatggt aactgcacgg cggagggccg ctacctcacc gcggactacc
     7021 cgggcgtgct gcgcgcgcgc ctgcaagcaa gccgaggcgg cgaagtgctc tagttcaacg
     7081 gacccctcgg cagccaggtg ggcccagggg ccgctccgac ctggtacgtc gatgaggcgc
     7141 acccggtcgg cgacggtctg acggtcccgg atggcgcggt gccgcttacc gagtgcgagg
     7201 accgggcacc ctatctttgc cgaagcttcg cgaagaccga gtccatcggc accgagctcg
     7261 ccaacgctgt gcgcgccgcg atggagcagt ccaagcctgt caccgtgacc gagctgagcg
     7321 tcgacatcga gagcttctac acgcgcctga ccaacattgg tttccgggtt ctgatcgccg
     7381 acggcgacat cggctggcag ccgccgactc tctacaactg catgggaaag cccttcagcg
     7441 atgacagctg cgtcgaggat ggcggagaga tcatcgacga ccccttgctc gcatttttcg
     7501 acagtcggat tgcccgtgga gacgtcatcg agtcgcagat cgctcgcgtc gattttggca
     7561 acgtgggcat gctgttcatg cccggcgaac tcccg
//
LOCUS       DASRZF010000226         5271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3247468, whole genome shotgun sequence.
ACCESSION   DASRZF010000226 DASRZF010000000
VERSION     DASRZF010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 5271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5271
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3247468"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1590
                     /locus_tag="VFG22_10080"
     CDS             <1..1590
                     /locus_tag="VFG22_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416630.1"
                     /translation="VAVKVVEIAGGRAYLAPGQAAGVGLGSVVVFGSRRYRVVGATDS
                     HAVVEMNGRELRVGMGGRAEAKVERSTATALPKPEPLEAYENEWPDPVLPATKQEPEY
                     VALGSWSDRKRIDLAVSTNVGGTLALSDGESFGRASLRTRLHAEPFAQPVFFDVDFAV
                     QSWFGGNIGDRPGNSSRPVLLVRDLQFGYGTSQTAQVGLGRLPYVSVGVGQLDGLRVR
                     SPSWAGFSIGAFGGTVPNPLDNMPSLDTTRFGAQLAYQNESLDARPFVGISVHGSTFD
                     GTLDERRLNAEFDVFPGTGHIAGHFELSLHDENNPWQAPRVEISMAGFDASVRIGLFQ
                     IAGRFDMRLPERSLWLTSYLPLGYLCNTVPDPAANAGDEVICLNSSDQRYFGGVDTSF
                     SFARWVLSAGASVVHSETADQFEQVVGYLQARILRIADIMWTDVSVAAYTRSFISDYA
                     ARLGVGVDIGRIGEISAFYRVSLNQYTAAPSGFLQQSAGGVVYLTLGHDLDLGLRVDG
                     MFGEDVRVLVLGSNLTWRPSW"
     gene            complement(1619..3052)
                     /locus_tag="VFG22_10085"
     CDS             complement(1619..3052)
                     /locus_tag="VFG22_10085"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HET6416631.1"
                     /translation="MIPVSTLDAAMLYAETAEMPMHTMGVLIFERDDDESDDQGDASS
                     FEQMRKLFAERIHLIAPFRRRIVQGPLQIGDPHWIEDPAFDLDKHLFNISLPSPGGMR
                     ELREFVGTFASQRLERDRPLWKVALVQGLEGNKRALVMKIHHAAMDGGRSAAIAGELL
                     DTSPEGRIIAPPDEEWIPDREPTIPWLAADTLRTLVGKPRRALEAAAKMASTLRATES
                     TRASRPPDEEPPEKPPLFEAPATMFNAALTPERSVSLVDVSFDDVKAIKAAFGTTVND
                     VVLAACAGSLRSWLAEHGGLPDQPLVATVPVSVRHDGDEAGNRVSVIRVHLPIQIDDP
                     VERLMAINQETVRQKKRHQSTGGGDILKNFADLVNNVTIPWITTHLIGLYSSSHLADR
                     VPPLWNLIISNIAGPAVPLYTKGARLTHLFPLGPVQHGSGLNITVMSAIDRLCFGALA
                     CTDLVPDVQDIGTGFVNEIEALKVRID"
     gene            complement(3049..4290)
                     /locus_tag="VFG22_10090"
     CDS             complement(3049..4290)
                     /locus_tag="VFG22_10090"
                     /inference="COORDINATES: protein motif:HMM:NF015892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HET6416632.1"
                     /translation="MKDSMVVSRIVHESYERARPLMPSGHWWEHLDDDFARRPEKFEL
                     ARLDRLQIGATAALDHVVRAVFGTMVGSTAIPFGFNPLELRRSMQNVGLYADMAESGD
                     RRRFFKDPPSGVRVRVKRGRWFPRFEPDDGVCEVLRFDSPFQPVHPSQHKPYLRHDRN
                     RVAYARMIRHRDGPRPTIVAIHGFTAEGYLINEWFFALPWFYRMGCDIALFTLPFHGP
                     RQTRFSPFSGHGFFAGGSSRFNEAVAQSVFDFRILFNWLRQEQGVEQVGVTGLSLGGF
                     ISSMLASVEENLAFALPNVPIISVADLVLEWQPLGALMRTALAASGLTAADARRLVAV
                     SCPLSYPPVLPPERLMIVGGAGDRLAPPKHSRLLWEHWQRCRMYWFPGSHSVHLDRGK
                     YLTEMARFMMGLGFLKPGSTR"
     gene            4399..5019
                     /locus_tag="VFG22_10095"
     CDS             4399..5019
                     /locus_tag="VFG22_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416633.1"
                     /translation="MRELSWPGALLGALTVLVPADLVAAQDDSAIDIITEPPAQTEPS
                     AVEPPAPELGKPKEPLGHLRVGGGIGLGFATDFFSIGISPQVSYIIKRIVEPGLGLTY
                     QFARDRVTIPETTWQTFGSSIFVRLYPIPQFFVLVEGELINTGWKQGDVSSGRMNYGN
                     LLLGGGLLMGVGKGVFVATSLKVPVLRNPFYPTAFPIIAAGVGYSF"
     gene            complement(5041..>5271)
                     /locus_tag="VFG22_10100"
     CDS             complement(5041..>5271)
                     /locus_tag="VFG22_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416634.1"
                     /translation="GYQHPEWGHAVWRDELEIGYESWKLDEVEPDDYCSIHVHNLVRA
                     KMGNKTGIGILETIVFGRHEPSGFKDLFDGAP"
BASE COUNT          993 a         1600 c         1632 g         1046 t
ORIGIN      
        1 gtggcggtca aggtggtcga gattgccggg ggccgcgcat atctcgcgcc cggtcaagcc
       61 gccggggtcg ggctcggatc cgtcgtcgtc tttggcagtc gccggtatcg cgtcgtcgga
      121 gcaacggatt cgcacgcagt cgtcgagatg aatggccgcg agcttcgggt gggcatgggc
      181 ggacgggccg aggccaaggt cgagcgatcc acggctaccg cgttgccaaa gcccgaacca
      241 ctcgaagcgt acgagaacga atggcccgat ccggtgcttc cagccacgaa acaggaaccc
      301 gagtacgtcg ctctcggaag ctggtcagac cgcaagcgga tcgacctcgc cgtttcgacc
      361 aacgtcggcg gtacgctcgc gctcagcgac ggagaatcct tcggacgcgc atcgctgcgc
      421 acccggcttc acgccgagcc cttcgcccaa cccgtgtttt tcgacgtcga tttcgcggtg
      481 caatcttggt tcggtgggaa catcggggat cgaccgggca actcctctcg ccccgtgctt
      541 ctggttcgcg acctgcagtt cggctacggg acgagtcaaa cggcgcaagt cggcttgggc
      601 agacttcctt acgtttccgt cggcgtcgga cagctcgatg gtcttcgtgt tcgctcgccg
      661 tcttgggcag gattttcgat cggtgcgttc gggggcacgg tgcccaaccc gctcgacaac
      721 atgccgagtc tcgacacgac ccggttcggc gctcagctcg cttatcagaa cgaatcgctg
      781 gatgctcggc ctttcgtggg aatcagtgtg cacggttcga cgttcgacgg cactctcgat
      841 gaacgtcgcc tcaatgctga attcgacgtg ttcccgggga ccggtcacat cgctggacac
      901 ttcgagcttt cgctacacga cgagaacaac ccctggcaag cgccgcgggt cgaaatcagc
      961 atggcaggct tcgacgcgtc ggttcgcatc gggttgttcc agatcgcggg tcgcttcgac
     1021 atgcgattgc cggaacggtc gctttggctc acctcgtatc ttccactcgg ttatctctgc
     1081 aacacagttc ccgaccccgc cgccaatgcg ggcgacgagg tcatctgcct caacagcagc
     1141 gatcagcgct acttcggcgg agtggacact agcttctcgt ttgcgcgctg ggttttgagt
     1201 gcgggcgcga gcgtcgtgca cagcgagacc gcagaccagt tcgagcaagt cgtcggctat
     1261 ctgcaggcac gaatcctgag aatcgcggac atcatgtgga cggacgtttc cgtcgccgcg
     1321 tacactcgct cgttcatttc cgactatgca gcccggctcg gagtcggcgt ggacatcgga
     1381 cggataggag agatctccgc gttctaccgg gtgtccctca accagtacac ggccgctcca
     1441 agcgggttcc tgcagcagtc cgccggaggc gtcgtctatc tcacgctcgg gcacgacctg
     1501 gatctcggcc tgcgcgtcga tggaatgttc ggggaagacg ttcgagtgct ggtgctcggc
     1561 tcgaatctga cctggcgtcc gtcctggtga cggcattgcg tgacgagcgc cctagccgct
     1621 agtcgatacg caccttgaga gcttcgatct cattcacgaa gccggtcccg atgtcttgca
     1681 cgtcggggac gaggtccgtg caagccaatg cgccgaaaca cagtcggtcg atggcgctca
     1741 tcacggtgat gttgagaccc gaaccatgct ggaccgggcc aagtggaaac aggtgcgtaa
     1801 gccgcgcgcc cttcgtgtac aaggggacag ctgggcccgc aatgttcgaa atgatcaagt
     1861 tccagagcgg cgggacccgg tcggcaaggt gtgacgagga gtagagcccg atcagatgag
     1921 tggtgatcca gggaatggtg acgttgttca cgaggtcggc gaagttcttg aggatgtcgc
     1981 caccaccagt gctttgatgg cgtttcttct gtcgcacggt ttcttggttg atcgccatga
     2041 gtcgctcgac cggatcgtcg atttggatcg gtaaatgcac gcgaatcacg gaaacccgat
     2101 tgccggcttc atccccatcg tgtctaaccg acaccggcac cgtcgccacc aatggctggt
     2161 cgggaagccc accatgctcg gcgagccagg atcgcaagct cccggcgcat gcggcaagca
     2221 ccacgtcgtt tacagtcgtg ccaaaggcgg ccttgatcgc tttcacgtcg tcgaacgaca
     2281 cgtccacgag ggaaacggaa cgctcgggtg tgagggctgc attgaacatc gtggccggag
     2341 cctcgaacag aggcggtttc tctgggggtt cctcgtcggg agggcgcgac gcacgagtcg
     2401 attccgtggc ccgtagtgtg gaagccatct tggctgcggc ttcgagcgca cgccgaggct
     2461 tgcccaccag tgtgcgcaag gtgtccgcgg cgagccaggg aatcgtaggc tcccgatcgg
     2521 gaatccactc ttcgtcgggc ggagcgatga tgcggccttc ggggctggtg tccaagagct
     2581 ctcccgcgat ggcagcactt cgacctccgt ccatggcggc atgatggatc ttcatcacga
     2641 gtgcgcgctt gttgccttct aggccctgta ccagcgcaac cttccacagg ggtcggtcgc
     2701 gttcgagtcg ctgggaagca aatgtcccca cgaattcacg gagctcgcgc atccctcctg
     2761 gagaggggag ggatatgttg aagagatgct tgtcgaggtc gaacgctgga tcttcgatcc
     2821 agtgaggatc gccaatctgc aggggccctt ggacgatccg tctccggaac ggcgcaatga
     2881 gatggatcct ttcggcgaac aatttgcgca tctgctcgaa tgacgaggcg tcaccctgat
     2941 cgtcgctctc atcgtcgtct cgctcgaaga tcaatacccc catcgtgtgc atcggcattt
     3001 cggccgtttc cgcgtaaagc atggccgcgt cgagcgttga aacgggaatc atcgcgtact
     3061 cccgggtttc aagaacccaa ggcccatcat gaagcgggcc atctcggtca ggtactttcc
     3121 gcggtcgaga tgcacgctat ggctccccgg gaaccagtac atcctgcagc gttgccaatg
     3181 ctcccagaga agcctcgagt gcttcggcgg cgcaagacga tcgccggcac cacctacgat
     3241 catcaagcgc tccggcggga ggactggtgg gtagctgaga ggacaactga ccgcgaccaa
     3301 ccgtcgcgcg tcggccgccg tcaggccgga ggctgcaagc gcagtgcgca tcagtgcgcc
     3361 gagcggttgc cattccagga ccagatctgc cacgctgatg atcggtacgt tggggagagc
     3421 aaatgccaag ttctcttcca cgctggcgag catcgaagag atgaatccgc cgaggctcaa
     3481 gccggtcacc cccacctgtt cgacaccttg ctcctgccgt agccagttga agagaattcg
     3541 gaagtcgaat acggactgcg cgacagcctc gttgaatcga gacgagcccc ctgcgaagaa
     3601 tccgtgaccc gagaagggtg agaagcgtgt ctgccgtggt ccatggaagg ggagcgtgaa
     3661 gagggcgatg tcgcatccca ttcggtagaa ccaaggaagc gcgaagaacc attcgttgat
     3721 caggtatccc tctgcggtga agccgtggat cgccacaatc gtcggtcgtg gtccatcgcg
     3781 gtgccgaatc attcgagcgt aggctacacg gttgcgatcg tgtcgaagat atggcttgtg
     3841 ttggctcggg tggactggct ggaacgggct gtcgaagcgc agcacctcgc aaacaccatc
     3901 atcaggctcg aaacggggaa accagcgccc gcgcttgacg cgcacccgga cgccggacgg
     3961 aggatccttg aagaagcgtc tgcgatcgcc ggactccgcc atgtcagcgt agagaccgac
     4021 gttttgcatc gaacgccgaa gctcgagcgg attgaaccca aagggaatgg ccgtcgaccc
     4081 taccatcgtg ccaaagaccg cacgcaccac atggtccaag gcggccgtgg ctccgatctg
     4141 caagcggtcg agtcgcgcca gctcgaactt ctccggcctt cgggcgaagt cgtcgtcgag
     4201 atgctcccac cagtgaccac tcggcatcag cggacgcgcc cgctcgtaag actcgtgtac
     4261 gatccgagac accaccatgg agtctttcat ggtggaagct tagcccagtc gcgctcgacg
     4321 gaaaggcgca atcgcgcggc ttccggcggg ccaagacctt cgaagatctc gaggattcgt
     4381 ttctgttata aagaaagcgt gcgtgagctt tcttggcctg gagctctctt gggagccctc
     4441 actgtgctgg ttccagcaga cctcgttgcg gcgcaggatg attccgcgat cgacatcatc
     4501 acggagcccc cagcgcagac cgagccgagc gccgttgagc cgcccgcacc cgagctcggc
     4561 aaacccaagg agccactcgg ccatcttcga gtcgggggtg gaatcgggct tggtttcgcg
     4621 acggacttct tctccatagg aatttcgccg caggtctcct acatcatcaa gcgcatcgtc
     4681 gaacccggtc tcggtttaac gtaccagttc gcgcgagacc gagtcaccat cccagaaacc
     4741 acttggcaaa ccttcgggag ctccatcttc gttcgactgt atccgattcc gcagttcttc
     4801 gtgctggtcg aaggcgagct gatcaacacc ggttggaaac aaggtgacgt cagctcgggt
     4861 cgcatgaact acgggaacct actgctcggt ggcggattac tgatgggtgt cggcaaaggg
     4921 gttttcgtcg cgacctcgct caaggtcccg gtgctgcgga atccctttta ccccacggcc
     4981 ttcccgatca tcgctgctgg cgtcggctac tcgttctaag aacgcgccgg agtcagcgtc
     5041 tcacggagcg ccgtcgaaca ggtccttgaa gccactcggt tcgtgtcgtc cgaagacgat
     5101 ggtttcgagg atgccgatac ccgtcttgtt acccattttg gcgcggacca gattgtgtac
     5161 gtgaatggag cagtagtcat ccggctccac ttcgtcgagc ttccagctct cgtatccgat
     5221 ctcgagctcg tcgcgccaga ccgcgtggcc ccattcaggg tgctggtatc c
//
LOCUS       DASRZF010000227         4569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_500895, whole genome shotgun sequence.
ACCESSION   DASRZF010000227 DASRZF010000000
VERSION     DASRZF010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500895"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1377)
                     /locus_tag="VFG22_10105"
     CDS             complement(<1..1377)
                     /locus_tag="VFG22_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416635.1"
                     /translation="MASPVDDRGIDAFLDLLGEGGKGPGPKSEALEALALRPVFLATW
                     EPRAEGFRTLINADGEEALAVFSSEDLLKSAASRFDWIGADGSIATHQSLGGDILRHA
                     WTREYAFVVIDIGSDHSLEYEREELKLILREMESTGPFRTSRPPAPSEKPAAESSFPP
                     PVERISTMYSMAPTQTETLDRLSDLPTKPHEVPFDSEPPTKPRTALPSKRPAPRKQPP
                     RPRQRKSSRPAQKPKSEPPSPQIEEVRVRNAELGDRGTYGAASIIPSSERRPQPISPS
                     MPVTEDEISRADSASASASAPAAPKLTSDAPTAPAAHPLPSLEPGRLKLPAIGPIGAG
                     PAEPPPPLDTKQTTAKTEKPPVPKGARVPVPSIGDGIKLVEVHEPPDEALLQSLAGVL
                     RGYFEIEWASYCEVARPAGPASPAVGLRITDGYRDNVTAIIKELCEASRKHDVEIDVL
                     LIDGHDMLR"
     gene            1492..1564
                     /locus_tag="VFG22_10110"
     tRNA            1492..1564
                     /locus_tag="VFG22_10110"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1525..1527,aa:Phe,seq:gaa)
     gene            complement(1607..3322)
                     /locus_tag="VFG22_10115"
     CDS             complement(1607..3322)
                     /locus_tag="VFG22_10115"
                     /inference="COORDINATES: protein motif:HMM:NF021597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HET6416636.1"
                     /translation="MSRFRPLLGFGPLLLLSMVSLPQGAKACGGLFCSATAPVNQAAE
                     RIIFSKNADGTVTAVVQIQYQGPSEEFAWVLPVPGIPEVKVSSDLAFTRLQLASNPQY
                     NLSTRVEGECLDDGTRFNLPTSGNADIDGSGGGGGAGGGGVTVLAQGNVGPYDYVVIK
                     PDGAFDRVGDVMVEWLTTEGYDVVPPGGDPGDISDLLGSYLEGGMNLIAFRLTKGNDE
                     GTIRPIRITYASDQPMIPIRPTAVAANDDMGVMVWVLGESRAVPVNYRSLQLNEALLD
                     WLTGGSNYNQVVVAAANEAGGQGFVTERAGEAAAYENVIVNDFERDQWESLEMTLPSL
                     SPEEVLRESAGFAQWDGYLGVLEDFIPEAELDAFIQCPQCSENLTPFDTGAFMNALAE
                     DVIAPMLETEALLGSRPYVTRLYTTLSAPEMDLDPLFDFNADLEDVTNVHNATRVIEC
                     SPFITFDEAPSRVELEDGRVVRLTPGNLTWPFTPDLKDAPPANAIVAQLTTSGPGTVV
                     TDNSDAIDAALARHNAQVPTLADMGLTTAGGCAVGQIQAGATLWLGLGLAALLLWRRR
                     ALRGR"
     gene            3492..>4569
                     /locus_tag="VFG22_10120"
     CDS             3492..>4569
                     /locus_tag="VFG22_10120"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HET6416637.1"
                     /translation="MQSRWSDEDAQEAIDRYRDEPRVNEDVALRVYTSRLIGQDPSLV
                     LHGGGNTSVKTRVVDDLGCEVDVLCVKGSGWDLGTIEPAGLPAIRLETLGPLRDLAAL
                     SDEDMVNAQRTRLVDSRAPNPSIETLLHAFLPHKFIDHSHADAILALLDQAQAETICR
                     EVYGGRLAFVPYVMPGFALAKLAVETFEENPAVEGLLLSKHGLFTFGASAKESYERHI
                     HAVDAAIRFASEKRARVRVARTIPAPLVYETIAPILRGRLGGGLRSYLLSIRQSAEID
                     AFLAREDLDAVSQRGTATPDHVLRTKRLPLLLDLDSAMDEAAIAKQIDERLTAYREDY
                     RAYVAEGSARKKVPITPLDPDPRII"
BASE COUNT          788 a         1439 c         1450 g          892 t
ORIGIN      
        1 gcggagcatg tcgtgaccat cgatgaggag cacgtcgatt tctacgtcgt gctttctgct
       61 ggcttcacag agctccttga tgatcgcggt gacgttgtcc cggtacccgt cggtgattcg
      121 aaggccgacc gcagggcttg ccgggccggc cgggcgcgcg acctcgcagt acgatgccca
      181 ttcgatctcg aagtaacctc gcaggacgcc ggcgagactc tggagcagcg cctcgtccgg
      241 aggttcgtgg acttctacca gcttgatccc atcgcctatc gaaggcacgg ggacgcgcgc
      301 gcctttgggt acgggcggtt tttccgtttt cgccgtcgtt tgcttggtat ccagcggggg
      361 cgggggttca gcagggccgg cacctatggg tccgatcgct ggcaacttga gcctgccggg
      421 ctccaaggat ggtagcggat gcgcagcagg cgccgtgggc gcgtcggagg tgagctttgg
      481 cgccgctggc gcagaggcgg aggcggaggc cgagtctgct cgggaaatct cgtcttcggt
      541 gacgggcatc gatggcgaga tcggttgcgg ccgccgctct gacgacggaa tgatgctggc
      601 cgcgccgtag gttccgcggt caccaagctc cgcgttgcgc acccgaactt cttcgatttg
      661 agggcttggc ggctcgctct tcggcttctg cgccggtcgt gagctcttgc gttgtcttgg
      721 acgtggcggc tgcttacggg gcgcggggcg tttgctcggc agcgcggtgc ggggcttcgt
      781 gggcggctcg ctgtcgaaag gcacttcgtg cggctttgtg ggcagatcgc tcaggcggtc
      841 gagcgtctca gtctgggtcg gcgccatgct gtacatggtc gaaatacgct cgaccggtgg
      901 cgggaacgag ctttcggcgg cgggcttctc ggacggagcc ggcggtctcg aagtgcggaa
      961 cgggccggtg ctctccatct cgcgcagaat tagcttgagc tcttcccgct cgtactcgag
     1021 cgagtggtcc gaaccgatgt cgatcaccac aaacgcatac tcccgagtcc atgcgtggcg
     1081 caggatgtca ccgccgagcg attgatgcgt cgcgatcgat ccgtccgctc cgatccaatc
     1141 gaagcgcgac gctgcgctct tcagcaagtc ttccgagctg aacaccgcca gagcctcttc
     1201 tccatcggcg ttgatcagcg ttcggaatcc ctccgcacgc ggctcccagg tcgctagaaa
     1261 gaccggtctt agcgcgaggg cttcgagcgc ctctgacttc gggccgggcc cctttccccc
     1321 ttcaccgaga aggtcgagga acgcgtcgat accgcgatca tccactgggc ttgccatccc
     1381 gagtcacacc atccagcatt cattgtaatc ctgaaggcgt accggaacgc aagttttctc
     1441 tgatgcttga caggccccca agcacggcta ggctccgcgc ctgttttccg ggcccagata
     1501 gctcagttgg tagagcagtg gactgaaaat cctcgtgtcc ctggttcgat tccaggtctg
     1561 ggcacccagc cccccgcagc tccggcccgc ggggggtgcc gtgcgtttag cggcctcgga
     1621 gggctcggcg gcgccagagg agcaaggcgg cgaggccgag acccagccag agcgtagcgc
     1681 cggcctgtat ctgccccact gcgcagcccc ctgccgtggt gagtcccatg tctgccaagg
     1741 tgggtacctg cgcgttgtgc cgagctagcg ccgcgtcgat cgcatcgctg ttgtcggtca
     1801 cgaccgtccc ggggccgctg gtcgtcagct gggctacgat cgcattcgcc ggaggcgcgt
     1861 ctttcaagtc gggcgtgaac ggccaggtca ggttgcccgg ggtcagccgt acgacccggc
     1921 cgtcctcgag ctccacccgt gacggcgctt catcgaacgt gatgaacgga ctgcattcga
     1981 tcacacgcgt cgcattatgc acgttcgtca catcttcgag gtcagcattg aaatcaaaca
     2041 aaggatcgag gtccatctcg ggtgctgaca aggtggtata gaggcgtgtt acgtacggcc
     2101 gtgatcccag aagcgcctcg gtctcgagca tgggcgcgat gacatcctcg gcgagtgcat
     2161 tcatgaaggc gccggtatcg aacggcgtca ggttctcact gcactgcggg cactggatga
     2221 acgcatcgag ctctgcctcc gggatgaagt cctcgagcac gccgagatat ccatcccatt
     2281 gagcgaagcc ggcgctttct cgtaggactt cctccggcga gagactgggg agcgtcattt
     2341 cgagcgactc ccactgatct cgctcgaagt cgttcacgat gacgttctca tatgctgccg
     2401 cttcgccggc acgttccgtc acgaatcctt gccctccggc ttcattggcg gcggctacga
     2461 cgacttggtt gtagttgctg ccgccggtga gccagtcgag tagggcttcg ttgagctgaa
     2521 gagatcggta gttgacggga acggctcggc tctcgccgag tacccagacc atcaccccca
     2581 tgtcgtcgtt cgcggccacc gccgtcggac ggatcgggat catgggttgg tccgacgcat
     2641 aggtgatccg gatcggccga atcgtgcctt cgtcgttgcc cttggtgagt cgaaacgcga
     2701 tcagattcat tccgccctcg agataggagc cgagcaggtc ggagatgtcc ccaggatcgc
     2761 ctcccggtgg aacgacgtcg tatccttctg tcgtcagcca ttcgaccatc acgtcgccaa
     2821 cgcggtcgaa cgccccatcc ggcttgatga ccacgtagtc gtatggtccg acgttgccct
     2881 gagcgaggac ggtcaccccg ccgccccccg cgccgccccc tccaccgctg ccgtctatat
     2941 cggcgttgcc gctcgtcggc agattgaacc gggttccatc gtcgagacat tcgccttcga
     3001 cccgggtgct caagttgtac tggggattgc tcgccaactg cagtcgggtg aacgcaaggt
     3061 cggacgaaac cttcacctct ggaatccccg gaaccggaag aacccaggcg aactcctccg
     3121 agggtccctg gtactggatt tggacgaccg cggtaactgt gccatccgca ttcttcgaaa
     3181 agatgatacg ttcggctgcc tggttcaccg gcgccgtggc cgaacagaag agcccgccac
     3241 aggccttcgc gccctggggg aggctgacca ttgagagcag cagaaggggt ccaaatccaa
     3301 gtagggggcg aaatcgagac atttttgtca tccttatctg gcgaccgggc gcagattggc
     3361 acacagatcg gtgattctac cagcaagtag agcaagcgac gtgccagcgg agcgagaggc
     3421 tcgcgggtcg atttgggcgc gatggaccca tgttaagctc cgccggccca cagctaggga
     3481 ggcagtccga cgtgcaaagc cgatggtccg acgaagatgc gcaagaggcg attgaccggt
     3541 accgcgacga gccgcgcgtc aacgaggacg tcgcgctccg ggtctatacc tcgaggctga
     3601 tcggccagga cccatcgctc gtgcttcatg gaggcggcaa cacttcggtg aagacccgcg
     3661 tcgtcgacga cctaggctgc gaggtcgacg tgctctgcgt gaagggcagc ggctgggacc
     3721 tgggcaccat cgagccggcc ggcttgccag caatccgtct cgaaaccctc ggccccctgc
     3781 gtgacttggc tgcgctctcc gacgaagaca tggtcaacgc gcagcgaacc cgacttgtcg
     3841 attcccgcgc gccaaatcct tcgatcgaaa cgctcttgca cgcgttcctg cctcacaaat
     3901 tcatcgacca ctctcacgcc gacgcgatcc tggccctgct cgatcaggcc caggcggaga
     3961 cgatttgcag ggaggtctac ggcgggcggc tcgcgttcgt accttatgtc atgcccggtt
     4021 tcgcgctcgc caagctagcg gtcgagacct tcgaagagaa tcccgccgtc gaaggtctgc
     4081 tcttgtccaa gcacggtctg ttcaccttcg gggccagcgc gaaggagagc tatgagcggc
     4141 acatccatgc ggtcgatgcg gcgattcgat tcgcttcgga gaagcgggcg cgcgtgcgtg
     4201 tcgctcgaac gattccggcg ccgctggtct atgagacgat cgcgccgatc ctccgtggaa
     4261 ggctcggcgg aggcctccgg agctaccttc tctcaatcag acaaagcgcc gagatcgacg
     4321 ccttcttggc gcgggaagac ctcgacgctg tcagccaacg tggaacggcg acgcccgacc
     4381 acgttcttcg caccaagcga cttcctttgc tcctcgatct cgactccgcc atggacgagg
     4441 cagcgatcgc gaagcaaatc gacgaacggc ttaccgcgta tcgcgaggac taccgtgcat
     4501 acgtggccga gggttccgct cgaaagaagg ttccgatcac acctctggat ccggacccca
     4561 gaatcattc
//
LOCUS       DASRZF010000228         5767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3250930, whole genome shotgun sequence.
ACCESSION   DASRZF010000228 DASRZF010000000
VERSION     DASRZF010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 5767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5767
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3250930"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="VFG22_10125"
     CDS             <1..808
                     /locus_tag="VFG22_10125"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HET6416638.1"
                     /translation="SKSGYYVYLRRGPSRRTQEDDALKVHITAIHERSQGTYGSPRIY
                     RRLRQKDFEVGRDRVARLMRELQRCGTPKRRYRCTTDSDHDAPVAPNLLQRDFEAKRP
                     NRRWVTDITYVWTSQSWMYLAVVMDLFSRRVVGWAMRPHLQAELALGALRMALGRRAP
                     EPGLVHHSDRGVQYAAHAYQNVLHQHGIVCSMSRKGDCWDNAVVESFFARLKTELLHR
                     QSWRTGREVQDAVARYIEGWYNPHRLHSSLGYLSPNQFEQRHFVNQAQAA"
     gene            complement(869..1972)
                     /locus_tag="VFG22_10130"
     CDS             complement(869..1972)
                     /locus_tag="VFG22_10130"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET6416639.1"
                     /translation="MLRLVLSDLHLGTGTRRGQLNPLEDFIHDDRFVELLAYYDHLSG
                     RDGSIELILNGDIFDLLKVKIADEWPTEVTEEIAIAKLQQCLEGHPKFVHSIREFLQK
                     PTNRVTYLPGNHDLELWFAGVQELFLRYVAPGNAAERVHFVTSSDTYYLPEGIQIRHG
                     HQFERIHRVDYSQMTRTLRDGTEILALPWGSLWILEVMNPLKEIRSHIDRIQPLRRFL
                     WSSALLDPGFVFKFLWRSTVYFLRHRVFTIRAWRHRIMNFPRLVREEVLEVTRGGYDE
                     QAIRALNKIRGAHTLIVGHSHGPRFLQLPNDRILVNTGTWVKMINLDLQYLGQDSGLT
                     YAVIGYDDDGKPETTLMRWQGAHEACEAIPYAD"
     gene            complement(2021..2773)
                     /locus_tag="VFG22_10135"
     CDS             complement(2021..2773)
                     /locus_tag="VFG22_10135"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HET6416640.1"
                     /translation="MEVVRAASVDSDLDQENRAVEASTAEELIEWASQRFGDKLIAST
                     SFGATSAVMLHLVSSVAPRTPVICVDTGYLFPETYQFAETLIKRLGLDVRFYSATMSP
                     ARQEALYGRLWEQGDEGVKRYLEINKVEPMQRALRELDADAWMAGLRSEQTAHRAGLR
                     RIDRQDGRVKIHPVLDWTSEHMTEYMERFDLPYHPMFEQGYRSIGDHHSTIPTTADMD
                     PRDGRILGKTRECGLHLPLTEEQNQSLKSSGL"
     gene            complement(2918..4255)
                     /gene="gorA"
                     /locus_tag="VFG22_10140"
     CDS             complement(2918..4255)
                     /gene="gorA"
                     /locus_tag="VFG22_10140"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HET6416641.1"
                     /translation="MAEYDYDLFVVGAGSGGVRAARVASELGARVAIAEEARMGGTCV
                     NVGCIPKKLYVYGSHFAYDLRDAESYGWSNESSFDWTILRQNKDREIARLNRIYEGLL
                     ERAGVRSIPGRARLLDAHTVEVEAARFTAGRILIATGGKAILPDIQGREHALTSNDVF
                     SLETLPKRVMMVGAGYIALELGSVFRALGVEVTLVHRGEEILRGFDRDLRCHLHDELE
                     VKGVRIILNTQVQELRKTNAGFEASLSNGETLETDFPVFAIGREPNVEGLGLEEIGVE
                     MGRRGGIVVDEDFTSSVSSIHAVGDVTDHVQLTPVALAEGMHFAHRFFGEGQHRVDYA
                     NIPTAVFCQPELATVGLTEAEAWHRCQDVRVYKSVFTPLKLSLSGRKEHSIVKLVVDA
                     SNDRVVGCHMAGHGAAEIIQGLAVAMKAGATKAQFDATMGIHPSSAEEFVTLR"
     gene            complement(4307..4972)
                     /locus_tag="VFG22_10145"
     CDS             complement(4307..4972)
                     /locus_tag="VFG22_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416642.1"
                     /translation="MTASLKEPVPSPAQELHPRDESATYSKRDEDFVRLLNAAGLACE
                     LDTGQLSAITATLEDSRIDSRRMDILEMYYRGNEDASIAARRRRSDRYFMHNDFFSAS
                     AHQLVSHLANLNPEIAPVTLQRIGANDGPLVLRAGEHFSAVTDEDDEDAELGTVAVRA
                     LVRALNALLAKTSVSERLVGLVPDASRELYVGVTEEGAMTLLQGGCTELSAPAALREF
                     ASW"
     gene            complement(5112..>5767)
                     /locus_tag="VFG22_10150"
     CDS             complement(5112..>5767)
                     /locus_tag="VFG22_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416643.1"
                     /translation="RASQDSLVARRDPFRHSFVMVSLRELLSLESPDLTAVASHLDAL
                     ESSERVAEIRTLGRAHQARLFDAAKGWRPITLADLVPIRRDAMQEVVHEGKNSLPLFN
                     HFAKAFVRPAPSDGKELWGYNRAGSFVETVVGPGYFVAYPFEVEGEVLVDYLRVPPKR
                     PEHWPPILPNSARLSRFVYNHTQDILRGVSAHVSIGRATKRGQPMSAWFVLCRQDPP"
BASE COUNT         1109 a         1782 c         1741 g         1135 t
ORIGIN      
        1 cagcaagagc ggctactacg tctaccttcg gcgtgggccg agtcggcgaa cgcaggaaga
       61 cgacgcgctg aaggtacaca tcaccgcaat tcacgagcgc agccagggca catacggcag
      121 tccgcgtatc tatcggcgac tgcgtcagaa ggacttcgag gtcggtcgcg accgcgtggc
      181 ccgtttgatg cgcgaattgc agcgctgcgg cacgccgaaa cggcgctacc gatgcacgac
      241 cgacagtgac cacgatgcac cggtcgcgcc caatctgttg cagcgtgact tcgaagcgaa
      301 gcgtcccaat cggcgctggg tcaccgacat cacctacgtg tggacctcgc agagctggat
      361 gtacttggcg gtcgtgatgg acctgttctc gcgtcgagtc gtcggctggg ccatgaggcc
      421 gcatcttcaa gccgagctcg cgctcggagc tctacgcatg gcgctcggac gtcgcgcgcc
      481 cgagcctggg ctcgtccacc actcggaccg cggcgtgcag tacgcggcgc acgcctacca
      541 aaacgtgctc catcaacacg gcatcgtctg ctcgatgagc cgtaagggag actgctggga
      601 caacgcggtc gtcgagagct tctttgcccg gctcaagacc gagctccttc accggcagag
      661 ctggcgaacc ggccgcgagg tgcaagacgc cgtcgcccgc tacatcgagg gctggtacaa
      721 cccgcaccgc ctccactctt cgctcggcta tctcagcccc aaccaattcg aacagaggca
      781 ctttgtgaac caggctcagg ccgcataatc aacccgtcca ctttttccag ggaagatcac
      841 agcgtcagtg ccagcgcccg tgccagtgtc agtctgcgta ggggatggct tcgcaggctt
      901 cgtgggcgcc ttgccatctc atgagcgtgg tctccggctt gccgtcgtcg tcgtagccaa
      961 tcacggcgta ggtcagtccg ctatcctgac cgaggtactg aaggtccaag ttgatcattt
     1021 ttacccaagt gccggtgttc acgaggatgc gatcattggg gagttgcaag aatcgcggac
     1081 cgtggctgtg gcccacaatc aacgtgtgcg cgccgcggat cttgttgagt gcgcgaatgg
     1141 cttgctcatc gtagccccct ctggtcacct cgaggacctc ctcgcgaacg agccgcggga
     1201 agttcatgat gcggtggcgc caagcccgga tcgtgaagac acggtgccgc aggaagtaga
     1261 cggttgagcg ccagaggaac ttgaagacaa agcccggatc caagagcgcg gacgaccaga
     1321 gaaatctccg cagcggctga atacgatcga tgtggcttcg aatctccttg agtggattca
     1381 tcacttcgag aatccaaagg ctgccccagg gcagcgcgag gatttcggtc ccgtcgcgca
     1441 gagtacgggt catctgagag tagtcgacac ggtgaatgcg ttcgaactgg tgtccgtggc
     1501 ggatctggat gccctcgggc aagtagtagg tgtccgagga cgtcacgaag tgcacacgtt
     1561 ccgctgcgtt ccccggcgcc acgtatcgaa gaaagagctc ctgcactccg gcgaaccaga
     1621 gctcgaggtc gtgattcccc ggtagatacg tcacgcggtt cgtcggcttc tgcagaaact
     1681 cccggatcga atgcacgaac ttggggtgtc cttcgaggca ctgctgaagc ttggcgattg
     1741 cgatctcttc agtaacttcc gtcggccact catcggcgat cttcaccttg agaaggtcga
     1801 agatgtcacc gttgaggatc agctcgatgc tgccatctct cccgctgagg tgatcgtagt
     1861 aggcgagcag ctcgacgaag cgatcgtcgt ggatgaagtc ctcgagaggg ttcagttgtc
     1921 cgcgccgggt tcccgtcccg agatgcaggt cgctgagaac gagccgaagc aaagtcaccc
     1981 tcgttcgagg ttacgcaaac cccggcgatg ggccagcttc ttacaatccg ctcgatttga
     2041 ggctttgatt ctgctcttcg gtcagcggaa ggtgcagccc gcattcccgt gtcttgccga
     2101 gaattcggcc gtcgcgcgga tccatgtcgg cagtggtcgg aattgtggag tggtggtcac
     2161 cgatcgaccg gtagccttgc tcgaacatcg gatggtaggg cagatcgaag cgttccatgt
     2221 actccgtcat gtgttcgctc gtccagtcga ggacggggtg gatcttgaca cgaccgtcct
     2281 gccgatcgat gcggcgcagc cctgcgcggt gcgccgtctg ttcggaacgc aggccggcca
     2341 tccaagcgtc tgcgtcaagc tcacgaagcg cgcgttgcat gggctcgacc ttgttgatct
     2401 ccaagtagcg tttcacaccc tcgtcgcctt gctcccaaag cctgccgtac agggcttcct
     2461 gccgagctgg cgacatcgtc gcggagtaaa agcgaacgtc gagacccaag cgtttgatga
     2521 gcgtttcggc gaactgatac gtttcaggaa agaggtagcc ggtgtcgaca cagatgaccg
     2581 gagtgcgggg cgcgacgctc gacacgaggt gcagcatcac cgccgaggtg gctccgaagc
     2641 tcgtcgacgc gatcagcttg tcgccgaacc gctgcgacgc ccattcgatg agctcttccg
     2701 ctgtcgatgc ctctacggct cgattttctt gatcgagatc ggaatcgact gaggctgcgc
     2761 gaacgacttc catgatggct cggactaact tgacaagttt actgcgggtt tgcaatgcgt
     2821 ccagtggcga agcgccaatc tagatcttaa atgccactaa acgcgtcaat ttatgtccgt
     2881 gcacgccatc tttattcttg attgattaag tcgtgaatca tcgcagcgtg acgaattctt
     2941 cagcggagct cgggtggatg cccatggtcg cgtcgaactg agccttggtg gcccccgctt
     3001 tcattgccac ggctagcccc tggatgattt cagccgcgcc gtggccagcc atgtggcaac
     3061 caaccacccg gtcgttcgat gcgtcgacga cgagcttgac aatgctgtgc tccttacggc
     3121 cactgaggct gagtttcagt ggcgtgaaca cggacttgta gacgcgcacg tcctggcacc
     3181 ggtgccaggc ctccgcctcg gtcagcccga cggttgcaag ctcgggttga cagaatacag
     3241 cggtggggat gttggcgtag tcgacgcggt gctgaccctc tccgaaaaac cggtgcgcga
     3301 agtgcatgcc ttccgcgaga gcgaccggcg tgagctggac atgatccgtc acatctccga
     3361 cagcatggat ggaggacacg gagctggtga agtcctcgtc gaccacgatg ccaccgcgcc
     3421 gacccatctc caccccgatc tcttcgagtc caaggccttc cacgttcggc tcacgcccga
     3481 tcgcgaacac cgggaagtcg gtttcgagcg tctctccatt gctgagactc gcctcgaacc
     3541 cagcattggt ctttcgcagc tcttgcactt gggtgttcaa gatgatccgc acgcccttga
     3601 cttcgagctc gtcgtgcaga tgacatcgca agtcgcgatc gaacccgcgt aagatctcct
     3661 cgccgcggtg aacgagcgtg acttcgacgc cgagagcgcg aaagacggag ccgagctcga
     3721 gagcgatgta gcccgccccg accatcatga cccgctttgg tagcgtctcg agtgagaaca
     3781 cgtcattcga agtgagggcg tgctcccgtc cctggatgtc cggcaagatg gcctttccgc
     3841 cggtcgcgat cagaattctt ccagccgtga accgggccgc ctcgacctcc actgtgtgag
     3901 catccaagag tctcgcgcgg cccgggatgg atctcacccc cgcgcgctcg agtagtcctt
     3961 cgtagattcg gttgagacgt gcgatctcac gatccttgtt ctgccgcaag atggtccaat
     4021 cgaagctgga ttcgttagac cagccatagc tctcagcatc tcgcaggtcg taggcaaaat
     4081 gagacccata cacgtagagc ttcttcggga tgcatccgac gttgacgcag gtgccaccca
     4141 tccgcgcctc ttcggctatc gccacccttg ctccaagctc gctcgcgacg cgagccgccc
     4201 tcacgccccc tgagcccgca cccacgacga agaggtcgta atcgtattca gccatttaca
     4261 ctcacctcga gtccgtggcc tgaggtatgg ctgagccgcg tctagattac cagctcgcga
     4321 attcgcggag cgccgcaggc gcggaaagct ccgtgcaacc accctgcagc agggtcatcg
     4381 cgccttcctc cgtcacgccc acgtagagct cgcgtgaggc gtcgggcacg agaccgacga
     4441 gcctttcgct cacggacgtt ttggccagta gcgcgttgag ggcgcgaacc agagcacgca
     4501 cggcgacggt tccgagctcg gcgtcctcgt catcctcgtc ggtgactgca ctgaagtgtt
     4561 ccccggctct cagaaccaat ggcccatcat tggccccgat ccgctgaagc gtcaccggcg
     4621 caatctctgg gttcaggttg gccaagtgag acacgagctg atgcgcgctc gccgagaaga
     4681 aatcgttgtg catgaagtag cggtcgctcc tgcgacggcg cgctgcaatg gacgcatcct
     4741 cgttgcctcg gtagtacatc tcgaggatgt ccattcggcg agagtcgatc cgcgaatcct
     4801 cgagggtcgc cgtgatcgcc gacagctgtc cggtatcgag ctcgcacgct aggccagccg
     4861 cgttcagcaa gcgtacgaaa tcctcatcgc gcttcgagta ggttgcgctc tcgtcacgag
     4921 gatgcagttc ttgggccggc gacgggaccg gctctttcag cgacgctgtc atgaccccct
     4981 cgcaattcag tgttcaacaa atggcgaaaa aggtcgaccc tctgacagag atcactcacc
     5041 gatcagcgcg aatcatcgat aattgagcgg atcgactccg gtgacccgca cagttgttca
     5101 ccatcgcccg cctagggcgg gtcctgccgg cacagcacga accaagctga cattggctgg
     5161 cctctcttgg tggcgcggcc gatggacacg tgcgccgaga cgccacgcag gatgtcctgc
     5221 gtgtggttgt acacgaacct cgataagcga gctgaattcg ggaggatcgg tggccagtgt
     5281 tcggggcgct tcgggggaac cctgaggtag tcgacgagca cttcgccctc gacctcgaac
     5341 ggatacgcca cgaagtagcc cggcccgacc accgtttcga cgaagctgcc cgcccgattg
     5401 tacccccaga gctccttccc atcgctcggg gcggggcgga cgaacgcttt ggcgaagtgg
     5461 ttgaacagag ggagtgagtt ctttccttcg tgaaccacct cctgcatcgc gtctctccgg
     5521 atcgggacga ggtcggcgag tgtgatcggg cgccagccct tcgcagcatc gaagagccga
     5581 gcctgatgcg cgcggcccaa ggtgcgaatc tctgcgactc gctccgacga ttcgagcgca
     5641 tcgagatgcg aggcaacagc ggtcagatcg ggcgattcga gactcagcag ctcacgcagg
     5701 ctcaccatga cgaagctatg tctgaacggg tcgcgacggg caactagcga atcctgcgat
     5761 gcccgtg
//
LOCUS       DASRZF010000229         4681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2908630, whole genome shotgun sequence.
ACCESSION   DASRZF010000229 DASRZF010000000
VERSION     DASRZF010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 4681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4681
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2908630"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VFG22_10155"
     CDS             <1..498
                     /locus_tag="VFG22_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416644.1"
                     /translation="IDLAILHEEGVRLVGRVGGVDGTRISFRDDLGRNIDAAEQKLDR
                     VLQRIDRFIDETCMAADIPDRRPPIAISEAPTELELRDAGISSVLWATGYRRRYPWLQ
                     VPVLNIRGEIIHDAGVTPAAGLYVLGLNFQRTRKSSFIDGVGDDARAVAEHLSQRFHR
                     SSIAA"
     gene            510..1682
                     /locus_tag="VFG22_10160"
     CDS             510..1682
                     /locus_tag="VFG22_10160"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET6416645.1"
                     /translation="MIMQPINNAYDVVIVGARCAGAATAMLLAALGLRVLVFDKSRLG
                     SDTVSTHALMRPAALLLHRWGLAERLEREGTPRIQRTAFRYADDRGTEVVEVDIRPRH
                     GVDALYAPRRTVFDRMLADAARDEGADVRHGVQMLDLLRTPAGRVCGVVLRDESGNVR
                     EIEAGLVIGADGRRSKVAQLVGATPYVWASHATSCAFGYFRNLPVDGNRWYYRPGMGA
                     GAIPTNHEETLVFASFSDDFVAGSAAERDRAFVRVIEESAPDIGARIRAAEQVGKLRF
                     FPGMRGFVRQPWGEGWALVGDAGYMTDPITAHGITNALRDADILARTIGYGGDLADYQ
                     MARDDLSLKFFELSDRVASLDWDIPTVKQYHRMMSQEMNREEEAMVAMEIASASAA"
     gene            complement(1669..2139)
                     /locus_tag="VFG22_10165"
     CDS             complement(1669..2139)
                     /locus_tag="VFG22_10165"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HET6416646.1"
                     /translation="MSTETNEVWPRGMPAIGQCAARTRTVEQRDIELFTEISGDRNPL
                     HYDEAVAKQTRFGEIVVQGGITSAILNAVVAEDLPGPGTVFLHVDWSFKAPVRPGDRI
                     TGHVEVLEVRTDKPITRLKTEVVRNDGTLVLSGEALCYTMPLGRQLRDSRSTQR"
     gene            complement(2146..3048)
                     /locus_tag="VFG22_10170"
     CDS             complement(2146..3048)
                     /locus_tag="VFG22_10170"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET6416647.1"
                     /translation="MNLRAAVLTGITLLAFAANSLLCRMALASDLIGPVVFSAIRLGS
                     GVAILVPLSALMTEPKHEACLSGSWKSGLALFVYAMAFSLAYVSLETGVGALILFGSV
                     QATMIGAALRQGERPRPQEWFGLVMAMAGLVYLVLPGMRAPNPLGAVLMLASGVGWGV
                     YSLRGKASSAPVAATAGNFARAFALAIALLIVGLFMSWNVQRASVRGAALAVISGALS
                     SGLGYVIWYIALRDLTATRAAIVQLAVPVIAAAAGIIVLGEEPSVRLAIASLMILGGV
                     GGAILARNHGAALDPCENSPERRP"
     gene            complement(3104..4615)
                     /locus_tag="VFG22_10175"
     CDS             complement(3104..4615)
                     /locus_tag="VFG22_10175"
                     /inference="COORDINATES: protein motif:HMM:NF019045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Na+/H+ antiporter"
                     /protein_id="HET6416648.1"
                     /translation="MKRFSLGLGKLAAVTTLWMLIAWPAIAAASGHAESPTIDFPPSL
                     DGYGDAGFASLGAILRNRVEKVPFNLWATLLFIGAIVHTFGTHRFRHISHVLEQRHDA
                     KIRAGETQNRVSALARVFHFLGEPEVVFALWTVPLFFTIDLFYDQATAASYINDGVNF
                     TEAVFVVVIMTIASTRPILELAEGAMRQLARLGGETPLAWWLVLLIVGSALGSFITEP
                     AAMTICASLLARKFYALKPSKALAYSTLGLLFVAISVGGTLTNFAAPPVLMVADAWGW
                     TTRHMLTQFGWKAMLGIVTATLAYYIYFRKELRELGRGALLRDPEEPAELPIPIWVTL
                     GHVLFMVLTVANAHHPKQLIFTILFFLAFVEVTEDFQRSFSLRSPVLVGLFLAGLVIH
                     GGLQQWWIAPVLGGLDELPLVLGATALTAFNDNAAVTYLCTLVPSMTEGMKYAAVAGA
                     VTGGGLTVIANAPNPAGQSILDKHFEDGISSLGLLAGALLPTLVVLGCFLAFR"
BASE COUNT          955 a         1451 c         1464 g          811 t
ORIGIN      
        1 atcgatcttg cgatccttca cgaggaaggc gtgcgactcg taggccgggt tggtggagtc
       61 gacggcacgc ggatctcgtt ccgcgacgat ctcggccgca acatcgacgc ggccgagcag
      121 aagctcgatc gcgtgctcca gcggatcgac aggttcatcg acgagacttg catggccgcc
      181 gacattcctg atcgtcgccc gccgatcgcg atttcggagg ctcccaccga gctcgagctg
      241 agagacgccg gtatttcgag cgtcctttgg gcaacggggt accgccgccg atacccgtgg
      301 ttgcaagtgc cggtcctcaa catccgcggg gagattattc acgacgctgg ggtcacccct
      361 gctgcgggcc tctacgtgct cgggctcaac ttccagcgca cccgcaagtc gagcttcatc
      421 gacggggttg gcgatgatgc tcgcgccgtt gccgagcatc tcagtcaacg ctttcatcgt
      481 tcttcgattg ctgcctagga gtcacgacca tgatcatgca acccatcaac aatgcctatg
      541 acgtcgtcat cgtcggagcc cgatgtgcgg gtgccgccac agccatgctt ctcgcagcac
      601 ttgggcttcg cgtgctggtg ttcgacaaga gccgccttgg ctccgacacc gtatcgacgc
      661 acgcgctcat gcgacccgcc gcgctgcttc tgcaccgatg ggggcttgcc gagcgactag
      721 aacgagaggg cacgccccgc atccagagaa ccgctttccg ctacgcggat gaccgcggca
      781 ccgaggtcgt agaagtcgac atcagaccac ggcacggcgt cgacgcgctg tacgctcccc
      841 gccggaccgt gttcgatcgg atgctggccg acgctgcgcg cgacgaagga gccgacgttc
      901 gccacggggt ccaaatgctc gatcttcttc gcacgcctgc cgggcgtgtg tgcggtgtcg
      961 ttctgcgcga cgaatcggga aatgttaggg agatcgaggc aggcctcgtc atcggagcgg
     1021 acgggcggcg ttccaaagtt gcgcagctcg tgggagcgac cccctatgtc tgggccagtc
     1081 acgccacgag ttgcgccttc ggttactttc gaaatcttcc agtcgacgga aaccgttggt
     1141 actaccgtcc gggtatgggc gcaggcgcca ttccgaccaa ccatgaggaa accctcgttt
     1201 ttgcttcgtt ttcggacgat ttcgtggccg gttccgcagc ggaacgcgac cgcgccttcg
     1261 ttcgcgtcat cgaggagagc gcgccggaca tcggggcccg tattcgggct gcggagcaag
     1321 tgggcaagct ccgtttcttc ccggggatgc ggggctttgt tcgtcagccc tggggcgaag
     1381 gttgggcgct cgtgggggac gccgggtaca tgacggatcc gatcaccgca cacggcatca
     1441 ccaatgcgct gcgagatgcg gacatcctgg cccggaccat cggctacggt ggcgacttgg
     1501 ccgactacca gatggcgagg gacgatctat ccttgaaatt cttcgagctc tccgatcgtg
     1561 ttgcatcgct cgactgggac atccccaccg tgaagcagta tcaccgaatg atgagccagg
     1621 agatgaaccg cgaagaggag gcgatggtcg cgatggagat tgcttccgct agcgctgcgt
     1681 agacctggag tcgcgaagct gccgacccaa gggcatcgtg tagcaaagcg cttccccgga
     1741 gagcacgaga gtcccgtcat tgcgaacgac ttcggtcttc agtctcgtga tcggcttgtc
     1801 tgtcctgacc tcgaggacct cgacgtggcc ggtgatccga tcgccggggc ggaccggtgc
     1861 cttgaagctc cagtccacgt gcaagaaaac ggtgccaggg ccgggcagat cctcggcaac
     1921 gactgcattg agaatcgcgc tcgtgatgcc gccttggacc acgatttctc caaaccgggt
     1981 ctgcttggcc accgcttcat catagtggag tggattgcga tcgcctgaaa tctcagtgaa
     2041 tagctcgatg tcccgctgtt cgaccgtgcg cgtgcgcgct gcgcattgcc cgatggcggg
     2101 catgccccgg ggccaaacct cattggtttc ggtgctcatg gtgggtcatg gtcgacgctc
     2161 cggcgaattc tcgcaagggt cgagcgctgc tccgtggttg cgggccagga tagcgccacc
     2221 cactccgccg aggatcatca ggctggcgat cgcgaggcgg acgctgggct cctcgccgag
     2281 gacgatgatt ccagcagcgg ctgcgatcac ggggacggcg agctgcacaa tcgcggcccg
     2341 ggtcgccgtc aagtcacgaa gcgcgatgta ccagatcacg tacccaaggc ccgagctgag
     2401 cgctcccgaa atcaccgcga gggccgctcc gcgtaccgaa gcccgttgca cgttccacga
     2461 catgaagaga cccacgatca gcaatgcgat tgcgagtgca aaggcacggg cgaaatttcc
     2521 cgccgtcgcg gccaccggcg ccgagcttgc cttcccgcgg agcgaataca cgccccatcc
     2581 gacccccgag gcgagcatca acactgcccc gaggggattc ggggcccgca tacctggcag
     2641 gaccaagtag actagcccgg ccatggccat gaccagaccg aaccattcct ggggtctggg
     2701 acgctctccc tgacgcaggg cggcgccgat catggtggcc tgcaccgagc cgaagaggat
     2761 cagggcgccc acgccggttt cgagcgacac ataggccaat gagaacgcca tcgcatagac
     2821 gaatagcgcc aggccagatt tccaagaccc agacaagcac gcctcgtgtt tgggctcggt
     2881 catcagagct gacaacggga cgaggatcgc gacgccgctg ccgaggcgaa tcgcggagaa
     2941 tacaacgggg ccgatgagat cggacgccaa cgccatccta cagagcagcg agtttgcagc
     3001 aaaggcgagc agggtgattc cagtgagtac ggcggcacgg aggttcacga cgccgacgat
     3061 actactagcc agtgcaaaaa tgtccgtcgt cctcgtgtga gcctcagcgg aaggcgagaa
     3121 agcagcccag cacgaccaag gtggggagca gagcgcctgc gagaaggccc agcgagctga
     3181 tgccgtcctc gaagtgcttg tcgaggatcg actgaccagc cggatttgga gcgttggcga
     3241 tcacggtcag cccacctccg gtcactgcgc ccgcgacggc cgcatacttc atgccttcgg
     3301 tcatcgacgg aaccagagta cacaagtacg tcaccgccgc gttgtcgttg aacgccgtca
     3361 gcgccgtggc gccaagcacc agtggaagct cgtccaagcc gcccaacacg ggggcgatcc
     3421 accattgctg aaggcccccg tgaatgacga gacccgccaa aaaaagtccg accagaacgg
     3481 gagaccgcaa gctgaagctt cgctgaaagt cttcggtcac ttccacgaac gcaagaaaaa
     3541 acagaatcgt gaagatgagt tgcttggggt ggtgtgcgtt cgctacggtc aggaccatga
     3601 agagcacgtg tcccagggtg acccaaatcg gaatcggcaa ctccgcaggc tcctctggat
     3661 cccggaggag cgcaccgcgc cccagttcgc gaagctcctt acgaaaatag atgtagtacg
     3721 cgagagtggc ggtcacgatg ccgagcatcg ccttccagcc gaactgagtc agcatgtggc
     3781 gggtcgtcca accccaggca tcggccacca tgagcacggg cggcgctgca aagttcgtca
     3841 acgtcccccc caccgaaatg gccacgaaca agaggccaag tgtcgaatac gccaaggcct
     3901 tgctcggttt gagcgcgtag aacttgcggg ccaagagcga cgcgcagatc gtcatcgcgg
     3961 ccggctccgt gatgaaggat ccgagcgccg aaccgacgat cagcaagacc aaccaccatg
     4021 cgagcggggt ctcgccgccc agtcgagcaa gctggcgcat ggcaccctcg gccaattcca
     4081 gaatgggcct agtcgacgca atggtcatga tcaccacgac gaacacagcc tcggtgaagt
     4141 tcacaccatc gttgatgtac gaggcggcgg tggcttgatc gtaaaagaga tcgattgtga
     4201 aaaatagcgg aaccgtccag agagcaaaga ccacttcggg ttcgccgagg aagtggaata
     4261 cacgtgcaag cgccgacaca cggttttgcg tttcgcccgc gcgaattttc gcgtcgtgac
     4321 gctgctcgag gacgtgcgaa atatgccgaa accggtgcgt tccaaaggta tgaacgatgg
     4381 cgccgatgaa cagcagagtg gcccagaggt tgaagggtac tttctcgacg cggttccgaa
     4441 ggatggcccc gaggctggcg aagccggcat ctccatagcc gtcgaggctt ggggggaagt
     4501 cgatcgttgg cgactcagcg tggcccgacg ccgccgcgat cgccggccaa gcgatgagca
     4561 tccaaagagt cgtcacggcc gccaatttgc ctaagccaag gctgaaacgt ttcacagcac
     4621 ccatctttcg cgcgtatgct atcagctgtc agaacaccgt gcccaccgac gtcactttcg
     4681 a
//
LOCUS       DASRZF010000230         7773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3596105, whole genome shotgun sequence.
ACCESSION   DASRZF010000230 DASRZF010000000
VERSION     DASRZF010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 7773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7773
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3596105"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="VFG22_10180"
     CDS             complement(<1..799)
                     /locus_tag="VFG22_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416649.1"
                     /translation="MENSTLSSTERLDLQRRIAAIAGERLGESQESIRQLEAILEVEA
                     EDAEAMAVLDRLYRAERRFADLRSLYERRLLSVTDPAEKWVLLNEVAQVEEEQLGDLP
                     AAAERHWKILESNPHDVDALRAVERLSQQLKQWDRLGAALERRLQSRVGDEDRLAVYL
                     QLADLRRIYLEDPAGALEHYRSALALDGRNEVAIAGLEALSAEGGGVGVEAIDLLEPA
                     YAKRGHFEKLAGLLRARLEKADDAEERHALKLRLADLSASELGDAAGA"
     gene            complement(914..3442)
                     /locus_tag="VFG22_10185"
     CDS             complement(914..3442)
                     /locus_tag="VFG22_10185"
                     /inference="COORDINATES: protein motif:HMM:NF024574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416650.1"
                     /translation="MSKLTVANYLGLLLEDPYDTKLVEGLRELLKSPEPANDAQDPLR
                     MLEAARGGHERRGEFLAASWLIELESELVSDDPEFQKVLVKELGRVRREELMDEQGAL
                     SAYEKLGGLDSEDPEVAEAVDQILQVEEKWAELARRFIEEARDAVDPRLKTSLLTRAA
                     SLMWQYGGDSTVEETDAIFDEALDADPSHMCTARQYALSLRARGRWDEVVSVFVRAAK
                     AARTREEKAAAWLQAARVLRRLLEDVEGAADAYQKVLELTPTDEEALGALVEHFTDLE
                     AWDELAAMYESALRSRQKLEAEKGMLLQLAMVHWRFRKDAEAAEPFFARLRKMDAAHP
                     GMLDFYRERIGSEDPSGRLLTILGDALRTATEPERKLALARELGHRAQKADRPERALE
                     AWKLVERLAPGDLDARAALQLLYQNSGKWNALSESIRGEIEALSQDSKEEKLRLLRDL
                     IPIYRDALELDSMLIQVYAEILTLSPFDEEALGALAELYESAGRWNELIHVLDQQAEI
                     VDDTQQKVELYVRIANLWIDRFGNLNQATGPLERVLRLQADHPSALAQLKDVYTTKRK
                     WDALFTVLGREAELASDPAERTKKKIEMAELCTERLHQNAIAIRLWKEILAEAPETGE
                     ALERLENLSEREKDWETLASVLESRAEAAVSDADQLAQLQRLGVVLMERLARTDDAIA
                     VWEHVLELDPGNSRVRRMLKDAYVAQGSWESLETLYGENEDWAGLAEVLGQAAERAEE
                     ADAIVGLSLRAADVYRERLDEPHRALRYYERALATDPSNVAAAKGVVPIYERDHKWPD
                     YVKALEVIVSGSGSETEIDLRLERIASLRSVYLQRLRDPEASLG"
     gene            complement(3638..4330)
                     /locus_tag="VFG22_10190"
     CDS             complement(3638..4330)
                     /locus_tag="VFG22_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416651.1"
                     /translation="MATLTALMLAAPLLGLSLQIDVSMLEAPAVIVDPGAVLAADFAI
                     ELNPPKEPSTADLIRKRNKIKKVHKWFGISTWSLMTLSVVSGFIQFHNQYGWWQSLGS
                     TPCVQGTAVFSQKACSGVPVGHATLVALTSAAYFTTFGLSFAMPDPLKVSEGNSDFAR
                     KLRTHKVLRWVTFAGMIAQIGLGIVTANADWFNIDRANNYKALRGIATGHLAVSLMTY
                     GAMTWAGAIMVF"
     gene            4439..5359
                     /locus_tag="VFG22_10195"
     CDS             4439..5359
                     /locus_tag="VFG22_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HET6416652.1"
                     /translation="MVSHSKLPARVSVYEMSPRDGLQNESAQVATHAKVRLIEALVDA
                     GIRRIEVGSFVAPKWVPQMADADEVCRMIERRPGVTYAGLCPNARGLERALAAKVDEI
                     AVFVSASETHNSKNVNKTSAETLKAFEPVIGPAVAQGLKVRGYVSTMWGCPYEGEVDP
                     RRGLELAKHLLGAGCYQISLGDTIGVGTPLQTEKILQLFTSAVAVEQIALHLHDTRGT
                     ALANVLVGLQAGVTTFDASVAGLGGCPYAPGAAGNLATEDLVYTLHGMGIETGIDLEK
                     LWQAGQVAEAIVGRELPGKVHRAGVRSLAK"
     gene            5374..6636
                     /locus_tag="VFG22_10200"
     CDS             5374..6636
                     /locus_tag="VFG22_10200"
                     /inference="COORDINATES: protein motif:HMM:NF014213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HET6416653.1"
                     /translation="MPVQDLLEYIDACPTPFHAVAETVTRLEAEGYRALDEADVWDVG
                     PGDKIYVVRGGGSVAAFHIGTAPYAQAGFRLLGAHTDSPNLRVKPNPELKQAGYGQLG
                     VEPYGGVLLHTWLDRDLSLAGRVALADGTTRLVDFGRELLRIPSLAIHLNRDVNVEGL
                     KLNAQKHMAPVLTMLNELGEVDLRSVLATEIGDIKAEDLLAWDLMAYDVQASTRSGLG
                     REFIHAARIDNLASCHAGLVALLGNQGAGDITRGVVLYDHEEVGSRSTQGAASNFLRS
                     CLERLCGTPAENFQRAVAKSFLISADMAHAVHPNYSDLHDPKHKPVLGEGPVVKINVN
                     QSYATDGESWASFERWAREAKVKTQRFVIRSDLRCGSTIGPITAAELGIRTVDVGNPM
                     LSMHSCREVAAAADVPKMIDVLKRFFHA"
     gene            6629..>7773
                     /locus_tag="VFG22_10205"
     CDS             6629..>7773
                     /locus_tag="VFG22_10205"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET6416654.1"
                     /translation="MPEHRRPPGPPNRLGLGNLRGMMGDPLAYMTAAAEYGPLVDFPI
                     LHERYVLVFDPDLIQQSLTQGHAVMRKDRFTHDLERVLGQGLVTSEDALWRKQRKLVA
                     HAFTPRRIRTYADSMVSAAQRSLDGWHHGQTLAIHKGMAELTLDVVGRTLFDADVRGA
                     AREVGHALEVISDFYAAVLDSPIQPPTWWPSPRMFRFRKAIRNVDAIIGRIIEERRRS
                     GEDRGDLLSALLAAQDDDGSAMSDRQLRDECVTLFLAGHETTSIALGNTLYLLSKHPE
                     IEARVHEEASAVLGSRRASSEDFARLELTERVIKESMRLYPPVYTIGRQLLSDFELGG
                     YTIPKGHMLLFVQWVTQRSDKCFVDPLRFDPDRWLPERAKAIHKYAY"
BASE COUNT         1444 a         2533 c         2345 g         1451 t
ORIGIN      
        1 acgcgcccgc tgcatcacct agctcggagg cagacagatc cgccagtcgc aacttgaggg
       61 catgtcgctc ttcggcatcg tccgccttct cgagacgcgc gcgcaagagc ccggccagct
      121 tctcgaagtg accgcgtttc gcgtacgccg gctcgagaag gtcgatcgct tcgacgccga
      181 caccgccacc ttccgccgac aatgcttcga ggccggcgat cgcgacttca tttctgccat
      241 cgagcgcgag cgcgcttcgg tagtgctcga gcgcgcccgc tggatcctcc agatagattc
      301 gtcggaggtc agcgagttga agatagacgg cgagtcggtc ctcatcgccc accctcgatt
      361 gcaagcgcct ttcgagcgcg gcaccgagtc tgtcccactg cttgagttgc tgtgacaagc
      421 gctcgaccgc tcgaagcgcg tccacgtcgt gaggattact ctcgaggatc ttccagtgtc
      481 gctctgccgc ggcgggcaag tcgccgagtt gctcttcctc gacctgcgcg acttcgttga
      541 gaagcaccca tttctcggcc ggatcagtaa cgctcaagag gcggcgttcg tagagcgagc
      601 gcaggtccgc gaaccgccgc tccgcacgat agagccggtc gagcaccgcc atcgcttctg
      661 cgtcttcggc ttcgacctcg aggatcgctt cgagctgccg aatcgattcc tgagactccc
      721 cgagccgctc gcctgctatc gcggcgattc tccgttgcag atcgaggcgt tccgtactgg
      781 aaagggtact gttctccaat cgatcgcgga agagtgcaac caagtccgcg tatgcgcccg
      841 ccgcttctgc tgattcctcg agaccggcaa cgatgtctgg atcggaaggc gccaggaggt
      901 acccacgggt ggctcagccg agcgaagctt ccggatcccg aagcctctgc aggtagacac
      961 tgcgaagcga tgcgatccgt tcgagccgca ggtcgatctc ggtttccgat ccggatcccg
     1021 atacgatgac ctccaacgct ttgacgtagt caggccactt gtggtcccgc tcgtagatgg
     1081 gcacgacgcc ctttgctgcg gcgacgtttg atggatcggt agcgagcgcg cgctcgtagt
     1141 agcgaagtgc gcggtgcggt tcatcgagac gctcgcggta cacgtcggcc gcacgaaggc
     1201 tgaggccaac gatcgcatcg gcttcctctg ctcgctccgc cgcctgaccg agaacttcgg
     1261 ccaatcccgc ccaatcttcg ttctcgccgt acagcgtttc gagcgattcc caactgccct
     1321 gcgcgacgta cgcatctttc agcattcgcc tgactcggct gtttcccggg tcgagctcga
     1381 gtacgtgttc ccagaccgca atcgcgtcgt ccgtccgggc gagtcgctcc atgaggacga
     1441 ccccgagccg ctgaagttga gcgagttgat ctgcgtcgct taccgcagcc tctgcacggc
     1501 tctcgagaac cgaggccagg gtttcccagt ctttctcgcg ctccgacaga ttctcgagcc
     1561 tttcgagggc ctcaccggtc tcgggcgcct cggcaaggat ctctttccag agccggatgg
     1621 cgatcgcgtt ctgatgcagc cgctcggtgc agagctccgc catttcgatc ttcttcttcg
     1681 tgcgctccgc cggatcgctc gcgagctcgg cctcacgacc gagcaccgta aacaaagcgt
     1741 cccactttcg cttcgtcgtg tagacgtcct tgagctgtgc caaggccgag ggatgatcgg
     1801 cctgcaaacg cagaacgcgc tcgagcggcc ccgtcgcctg attgagattt ccgaaccgat
     1861 cgatccatag gttcgcgatc cgtacgtaga gctcgacctt ctgctgtgtg tcgtcgacta
     1921 tctccgcctg ctgatcgagc acgtgaatca gctcgttcca tcgaccggcg ctttcgtaga
     1981 gctcagccag ggcgcccaac gcctcctcgt cgaacggaga cagcgtcagt atctccgcat
     2041 aaacctgaat cagcatcgaa tccagctcga gcgcatcacg gtagatcggg atcaagtccc
     2101 gaagcagccg aagcttctcc tccttcgaat cctgggacag ggcttcgatc tctccgcgaa
     2161 tcgactcgga cagcgcgttc cacttgccgc tgttttgata gagcagctga agagctgcgc
     2221 gggcgtcgag gtcgcccggt gccaaacgtt cgaccaactt ccacgcttcg agcgcgcgtt
     2281 cgggacgatc ggctttctgt gcccgatgcc cgagctcgcg tgccagcgcg agctttcgct
     2341 cgggttcggt cgcggtgcgc agagcgtctc cgaggatcgt gagcagccgg ccgcttggat
     2401 cttcgctgcc gattcgctcg cgatagaagt cgagcatccc tggatgcgcg gcgtccatct
     2461 tgcgcagcct cgcgaagaac ggctccgccg cctctgcgtc tttgcgaaag cgccaatgca
     2521 ccatcgccaa ctggagcagc atgccctttt ccgcttcgag cttctgacgc gagcgaagcg
     2581 cactctcata catggctgcg agctcgtccc aggcctcgag gtcggtgaag tgctccacga
     2641 gcgctcccag cgcttcttcg tcggtgggcg taagctccaa gaccttttga taggcgtcgg
     2701 cggcgccctc gacgtcctct agcaagcgtc gcaggacgcg cgccgcctga agccaggccg
     2761 ctgccttctc ttcgcgagtc cgtgcagcct tcgccgcgcg aacgaatacc gaaaccacct
     2821 cgtcccagcg gccacgcgca cgcaagctga gggcgtactg tctcgcagtg cacatgtgcg
     2881 aggggtcggc gtccaaggcc tcgtcgaata tcgcgtcggt ttcctcaacc gtcgagtcgc
     2941 ctccgtactg ccacatcagc gaagcggccc gcgtgagcaa ggaggtcttc agccgcgggt
     3001 cgactgcgtc gcgtgcctct tcgatgaagc gacgcgctag ctcggcccac ttctcctcga
     3061 cctggagaat ctgatcgact gcttccgcga cctcggggtc ctccgaatcg agaccaccaa
     3121 gcttctcgta cgctgagagc gcaccctgct cgtccatgag ctcctcgcgc cgaacgcgtc
     3181 ctagctcttt gacgagaacc ttctggaact ccgggtcgtc ggagaccaac tccgactcga
     3241 gctcgatcag ccaggatgcc gcgaggaatt caccgcgccg ctcgtgtccg ccgcgcgccg
     3301 cctcgagcat gcgaagcgga tcctgcgcat cgttggcagg ctcgggactc ttgagcagct
     3361 ccctcagacc ttcgacgagc ttggtgtcat aagggtcttc aaggagaaga cccaggtagt
     3421 tggcgaccgt caacttggac actccaactc ctgctcgttg attcggaagg atttttgcac
     3481 ccttcggtga tctcatgaaa cccccaaaag caaaaggatc ctgcgaccta agctccccac
     3541 tgcgcaggcg acttagatgt tagccgcgcg gcgatcctat gggcaagcct cgaattctgg
     3601 gaaattcgag caaatgccga tgttggtgag atcgctctca gaagaccatg atggcgccgg
     3661 cccaggtcat ggccccgtag gtcatgaggc tgacggccag gtggccggtc gcaatcccgc
     3721 ggagcgcctt gtagttgttg gcccggtcga tgttgaacca gtcggcgttc gccgtgacga
     3781 tcccgaggcc gatctgtgcg atcatcccgg caaaggtgac ccagcgaagc accttgtggg
     3841 tgcgtagctt ccgcgcgaaa tcgctattgc cctcactcac tttgagcggg tcaggcatgg
     3901 cgaaagagag cccaaaggtc gtgaaatatg cggccgaggt cagtgcgacc agcgtggcat
     3961 gcccgacggg aaccccgcta caggcctttt gcgagaagac cgctgtccct tggacacaag
     4021 gcgtgctgcc caggctctgc caccagccgt actggttgtg gaactgaatg aacccggaca
     4081 cgaccgacag ggtcatcaag gaccaggtgc tgattccgaa ccacttgtgg actttcttga
     4141 tcttgttgcg cttccgaatc aggtcggccg tcgagggttc cttgggcggg ttgagctcga
     4201 tcgcgaagtc ggcagccaag acggcgcctg ggtccacgat gacggccggt gcctcgagca
     4261 tcgaaacatc gatctgcaag gacaatccca gcaagggcgc agcaagcatg agagcggtca
     4321 atgtcgccat ggcgggcgga gtatctacca aattgggcta ttaggccagg caactgccga
     4381 atcgaggcac ctcgaccacc ggcttttcac ggcgcgccac tcgcagtaga tcgaggatat
     4441 ggtctcccat tcgaagcttc ccgcccgcgt gtccgtgtat gagatgtccc cgcgagatgg
     4501 gctccagaac gagagcgctc aggtcgccac gcatgcgaaa gtaaggctca tcgaggcgct
     4561 cgtcgacgcc ggcatccgcc gaatcgaagt cgggagcttc gtagccccca agtgggttcc
     4621 acaaatggcg gacgcagacg aggtctgccg gatgatcgag cgtcggccgg gcgtcaccta
     4681 cgcgggcctc tgccccaatg cgcggggcct tgaacgggct ctggccgcca aggtcgacga
     4741 gatcgccgtc ttcgtgagcg ccagcgagac gcacaacagc aagaacgtca acaagacgag
     4801 cgcagagacg ctcaaagcgt tcgagccggt catcggaccc gctgtcgccc aggggctcaa
     4861 agtgcgcggc tacgtgtcga cgatgtgggg ctgcccctac gaaggcgagg tcgacccgcg
     4921 acgcgggctc gagcttgcca agcacttgct cggcgcgggt tgctatcaga tctcgctcgg
     4981 ggacacgatc ggcgtgggca ccccgctcca gaccgagaaa atcctccagc tcttcacgtc
     5041 tgcggtcgcg gtggaacaga tcgccctgca cctccacgat acccggggca cggcgctcgc
     5101 gaacgtcctg gtgggcttgc aggctggcgt gacgaccttc gacgcctcgg ttgcggggct
     5161 cggaggctgc ccctacgccc cgggcgccgc tggcaacctc gcaaccgagg acctggtcta
     5221 cacgcttcac ggcatgggta tcgaaaccgg catcgatctg gaaaagctct ggcaagccgg
     5281 ccaagtcgcg gaagcgattg tcggtcgcga gcttcccggt aaagtgcacc gcgccggtgt
     5341 gcggtcgctg gcgaaataga ccaagggagc caagtgccag ttcaagatct actcgaatac
     5401 atcgacgcct gccccacccc ctttcacgcc gtcgccgaaa cggtcacacg acttgaagct
     5461 gagggctatc gagcgctcga cgaagcggac gtctgggatg tcggccccgg ggacaagatc
     5521 tacgtcgtac gcggcggcgg ttcggttgcc gctttccata tcggtacggc gccctacgcc
     5581 caagcgggtt tccgcctgct cggggcgcac acggattcac cgaacctacg cgtcaaaccc
     5641 aatcccgagc tcaagcaagc cggttacggg caactcggag tcgagccgta tggcggggtt
     5701 ctcttgcaca catggctcga tcgggatctc tcgcttgctg gccgggtcgc tcttgcagac
     5761 ggaaccacga ggctcgtgga ttttggccgg gagctgctgc gcatcccctc gcttgcgatt
     5821 cacctgaatc gcgacgtcaa cgtcgaaggc ctcaagctca atgcccaaaa acacatggcg
     5881 cctgtcctga ccatgttgaa cgagctcggc gaagtggacc tgcgcagcgt cctagcgacc
     5941 gaaatcgggg acatcaaagc agaagacctg ctggcctggg acctcatggc ctacgacgtg
     6001 caggcttcga cgcgaagcgg cttagggcga gagttcatcc atgcagcgcg catcgacaac
     6061 ctcgcgagtt gccatgccgg cctggtcgcc ctactcggca atcaaggcgc tggcgacatc
     6121 acgcggggcg tcgtcctcta tgaccatgaa gaggtcggta gccgaagcac tcagggcgct
     6181 gcgtccaact tcctgcgcag ctgcctggaa cggctctgcg gcaccccggc ggaaaatttt
     6241 cagcgcgcgg tcgcgaagtc gttcttgatt tctgccgaca tggcccacgc ggtacacccg
     6301 aattattcgg atcttcacga tccaaagcac aagcctgtcc tgggcgaagg cccggtggtc
     6361 aagatcaacg tcaaccagtc gtatgcgacc gatggcgaga gctgggcgag cttcgagcga
     6421 tgggcacgcg aagcgaaggt gaagactcaa cgcttcgtga ttcgttcgga cctgcgttgc
     6481 ggcagcacga tcggtcccat cacggcggcc gagctaggca ttcgaacggt cgacgtcggc
     6541 aatccgatgc tctccatgca ctcgtgtcgc gaagtcgccg ccgcagcgga cgtgcccaag
     6601 atgatcgacg tcctgaagcg cttctttcat gcctgagcat cggagacccc caggaccacc
     6661 aaaccgcctg ggtctcggca acttgcgcgg gatgatgggc gacccgctcg cgtacatgac
     6721 cgcggccgct gagtacggcc cgctcgtcga cttcccgatc ctccacgagc gctacgtgct
     6781 ggtgttcgat cccgatctca tacagcaaag cctgactcag gggcatgcag tgatgcgcaa
     6841 ggaccgcttc acgcacgatc tcgaacgcgt cctcggtcag ggcctagtca cgagcgagga
     6901 cgcgctctgg cgcaagcaac gaaagctcgt agcgcatgct ttcactccgc ggcgcattcg
     6961 aacgtatgcc gactcgatgg tctccgcagc gcagcgctcg ctcgacggct ggcaccatgg
     7021 tcagacgctg gccatccaca aaggcatggc cgagctcacg ctagacgtgg tcgggcgcac
     7081 gctcttcgac gccgatgttc gtggcgcggc ccgggaggtc ggacatgccc tggaggtcat
     7141 cagcgacttc tacgctgcgg tcctagacag ccccattcaa cctccaactt ggtggccctc
     7201 cccgcgcatg ttccgcttcc gtaaagcgat tcgaaacgtg gacgcgatca tcggcagaat
     7261 catcgaggag aggcggcgaa gcggagagga ccgaggggac ctgctgagcg cgctcctcgc
     7321 cgcacaggac gacgacggca gcgccatgag cgacaggcag ttgcgcgatg aatgcgtgac
     7381 gctcttccta gccgggcacg aaaccacctc gattgcccta ggcaacacgc tctatctcct
     7441 ctcgaagcac ccggaaatcg aagctcgcgt ccacgaagag gcgagcgcgg tcctcgggag
     7501 ccgaagggcg agctccgagg acttcgcgcg cctcgagctc accgagcggg tgatcaagga
     7561 atcgatgcgc ctttatcccc cggtctacac gatcgggcgg cagctcctct cggatttcga
     7621 gctaggcggc tacacgatcc caaagggaca catgcttctg ttcgtccagt gggtcacgca
     7681 gcggagcgac aagtgcttcg tcgatccact ccgattcgac cccgatcgct ggctcccaga
     7741 gcgcgccaag gcgattcaca agtacgcgta ctt
//
LOCUS       DASRZF010000231         4458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3596627, whole genome shotgun sequence.
ACCESSION   DASRZF010000231 DASRZF010000000
VERSION     DASRZF010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 4458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4458
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3596627"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VFG22_10210"
     CDS             <1..289
                     /locus_tag="VFG22_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416655.1"
                     /translation="LDPRLAYWALPLAEYGLQGQPTPNSVLVCAPMSVEDGEVIDTGS
                     DCETLDANDFIHDGASLIVPVAPSAWLSSDPLNGLYYTEVNFEFGPIYLCP"
     gene            387..860
                     /locus_tag="VFG22_10215"
     CDS             387..860
                     /locus_tag="VFG22_10215"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HET6416656.1"
                     /translation="MYTKLIKWFAQTRVGGWMFLNVFDKIDKPLMRLTNARLSTGIGT
                     ALSKNAILLTCTGAKSGVERDVALLCTPSGDDIVLVASRTGHANNPGWYYNLKAHPHC
                     VVRFRGKKFACVAKEATGAQREELWKLATEQYPGYDTYATRTARVIPVMLLVAAP"
     gene            complement(1135..2760)
                     /locus_tag="VFG22_10220"
     CDS             complement(1135..2760)
                     /locus_tag="VFG22_10220"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HET6416657.1"
                     /translation="MSAEYDYIVIGSGSSGGIVASRLSEDPTVRVLLLEAGGSDRTAL
                     IRKPGMGGLVHMVKPLKKKLDWGFRTVPQAHMNQRRVPYTRGRVLGGSSSINGMLYLR
                     GHRQNYDDWAAAGCEGWSHEEVLPYFKKLESHEDGETAYHGATGPIRVTRHPADQVSP
                     VSSAFLEAASEVCRIPIGDDFNAENQHCASMFHMSAADGVRCSVGECYVAPATERANF
                     HVETRALVRRVVVENGRATGVEYLRGSSTQLVRATREVIVSGGAIGSPQILMLSGIGP
                     AEHLREHGIEVEIDLPGVGQNLHDHLFVPITYRSPTSRHRGTALHFLGGMIQEYLLGG
                     GWFGRMNFEAGAFIKSEESAPIPDVQLHTMPWGYFTPNQDGPGRPDLDKGHCLSILVT
                     LIYPNSRGELRLSSDRPDQEPHIDPAFLAEPEDTQVLIRAIRKTREICTHRAMSGHIA
                     EELSPGAARASDQELVEEIKLRGCTGYHPVGTCKMGVDPMAVVDPSLKVHGVEGLRVA
                     DASVMPLVPGGNTNAPCMMVGERAASLILGQTS"
     gene            complement(2910..3158)
                     /locus_tag="VFG22_10225"
     CDS             complement(2910..3158)
                     /locus_tag="VFG22_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416658.1"
                     /translation="MILCSSALARRVLHGAAGMLDLDLTFVAFAAALLLGSCAAWAVA
                     LWLVEKRLGDSFQRQLNAARREAERRQEEQTADPLKRR"
     gene            3199..4125
                     /locus_tag="VFG22_10230"
     CDS             3199..4125
                     /locus_tag="VFG22_10230"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HET6416659.1"
                     /translation="MSERRQPLRDGLPSMVDGRRLGSMGRIVVEDIEEYSSVELAKKY
                     KSTAGSQPKADPNEVDRCMEAIERDGFAVIENRISPELVAEIKEDLIPRFEYESGRNN
                     FEGFKTQRLYAFFEKSLVCNPLVEDSLILGCLDRIFEPNYLLSQLQAINILPGESQQP
                     LHPDDAFYRVPRPRPPLGAATIWAIDEFTEENGATVVIPKSHLWDDRLPTEADLKDLQ
                     PVVMPAGSVVFFVGTLWHGGGANRSDAARLCVSAQYCAPWCRTQENYSLSLSRETVKR
                     CSEHIQRMLGYSIHPPFMGFVNGKHPKRLLED"
     gene            4202..>4458
                     /locus_tag="VFG22_10235"
     CDS             4202..>4458
                     /locus_tag="VFG22_10235"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HET6416660.1"
                     /translation="MAMSIPVWVLLAFAVWTLLVLVLTVGIYRWGLIFAGRVQVKEFR
                     ADQIEGSDRYRRSMRAHLNCVENLPVYGAIVLVIVVSGAQSS"
BASE COUNT          825 a         1456 c         1360 g          817 t
ORIGIN      
        1 gttggaccct cgactagcgt actgggcact cccgctcgcc gaatatggct tacaggggca
       61 accgactccc aactcggtgt tggtgtgtgc tccgatgagc gtggaagacg gtgaggtcat
      121 cgacaccggc tcggactgcg aaacgctcga cgccaacgat ttcattcacg acggtgcctc
      181 cctcatcgtc cccgtcgcgc catccgcatg gctatcatcc gatccgctga acggcctcta
      241 ctacaccgag gtcaacttcg agttcggacc gatctatcta tgcccgtgac ttgccgggcc
      301 gttgctcgtg cgcgggcagg ccaacggcca gcgcaagatt cgcaggcggg gacaaaaagg
      361 gcgtttggat ttactatgag cccatcatgt acaccaaact catcaaatgg ttcgcgcaaa
      421 cgcgcgtggg cggctggatg ttcctcaacg tgttcgacaa aatcgacaag ccgttgatga
      481 ggcttactaa cgcgcgtcta tcgacgggta tcggcacggc cctgagcaag aatgcgatcc
      541 tgctaacttg caccggcgcc aagtccggag tcgaacgtga tgtagcgttg ctttgtaccc
      601 ccagcggcga tgacatcgtg ctcgttgcgt cgcgcaccgg ccacgccaac aaccccgggt
      661 ggtactacaa cctcaaggcc catccgcact gtgtggtgcg ttttcgcggc aagaagtttg
      721 cctgcgtcgc aaaggaagct acgggtgctc agcgcgaaga gctttggaag ctcgcaaccg
      781 agcagtaccc gggctatgac acgtatgcga cgcggacggc gcgagtgatt ccagtgatgt
      841 tgttggtggc ggcgccgtga ggggacacgc gacccccttc cgagctgtct gatattgggc
      901 acttaggcct tgaaccggcc gtctgagcac ggcgacgtag ccaacacctg cctgcctgcc
      961 ctacgcagac tctcacgccg ccagcctccg tagcccatcg caatccgtga ggaaccccac
     1021 cacttggttc caactcagca tcattgggta cgccgccgct cgggcccgga gtagatgctc
     1081 cgggcccttt ttcttttctt ggggtcgacg cgagcaacgc aaggggtggg atgcctagct
     1141 cgtctgcccc agaatcaggg aagccgcccg ctccccaacc atcatgcaag gcgcattggt
     1201 gttgccgccg ggcaccaagg gcatgacgga ggcgtcggcg actcgaagtc cctccacgcc
     1261 gtgaaccttg aggctcgggt cgacgacggc catcggatcg acgcccatct tgcacgtgcc
     1321 gaccgggtga taccccgtgc acccgcggag cttgatctcc tcgacgagct cttgatcgct
     1381 cgcacgcgcg gcccctgggc tcagctcctc agcaatgtgc cccgacatcg cccggtgcgt
     1441 gcagatctct cgagtcttgc gaatggcgcg aatcagcacc tgcgtatctt cgggttcggc
     1501 gaggaacgcg ggatcgatgt gcggctcctg gtccggccgg tccgagctca ggcgcagctc
     1561 accgcgactg ttcgggtaaa tcagcgtcac cagaatggaa agacagtgcc ccttgtcgag
     1621 gtccggcctc cccggcccat cctgattcgg cgtgaagtag ccccaaggca tcgtgtggag
     1681 ctgcacgtcg gggatcggag cactctcctc gctcttgata aaggcgccgg cttcgaagtt
     1741 catgcgcccg aaccaaccgc caccgaggag gtactcctgg atcatccctc ctaggaaatg
     1801 aagggccgtc ccgcgatgac gcgaggtggg cgagcgatac gtgatcggca cgaagaggtg
     1861 atcgtgcagg ttctgcccca cccccggcag gtcgatttcg acctcgattc cgtgctcacg
     1921 caggtgctcg gccggcccga tgcccgagag catgaggatc tgcggcgagc caatcgcgcc
     1981 gccggacacg atcacctcgc gcgtcgcgcg caccaactgt gtgctgctgc ctcgcaggta
     2041 ctcgacgccg gtggcacgcc cattctcgac gaccacgcgc cgcaccagag ccctcgtttc
     2101 gacgtggaag ttcgcgcgct ccgtggcggg cgcgacgtaa cactccccga cgctgcagcg
     2161 aaccccgtcc gccgccgaca tgtgaaacat gctcgcgcaa tgctgattct cggcgttgaa
     2221 gtcgtccccg atgggaatcc ggcagacctc ggacgctgcc tcgagaaagg cactcgaaac
     2281 ggggcttacc tggtcggccg ggtgacgcgt cactcgaatc gggcccgtcg cgccgtgata
     2341 cgccgtctcc ccgtcttcgt ggctctcgag cttcttgaag tagggaagca cctcttcgtg
     2401 gctccacccc tcacaacccg cagccgccca gtcgtcgtag ttctgccggt gaccgcggag
     2461 gtagagcatc ccgttgatgg agctcgagcc gccgagcacc cttccgcgcg tgtagggaac
     2521 gcggcgctgg ttcatgtgcg cctgcggaac cgttcggaag ccccagtcga gcttcttctt
     2581 gagtggcttc accatgtgca ccaagccgcc catccccggc tttcgaatca gcgccgtccg
     2641 gtcgctcccc ccggcctcga gaagaagcac gcgcacagtc gggtcctccg acaggcggct
     2701 cgccacgatg ccacccgacg atccagaacc aatcacgata tagtcgtact ctgcgctcat
     2761 gggtccgagc atagcggctt catcgaacga cgagaacggg ctgcagtcgc agagcgcgac
     2821 cgggtagaca cttgagggct tgcctgccgc aagcctcccc tcggcggtcg aaagccgcta
     2881 gttcagcgaa cccacgacac cggctttgct caacgccgtt tcaacgggtc cgccgtctgc
     2941 tcctcttgac ggcgctctgc ctcgcgtcga gccgcgttga gctggcgttg gaacgaatcg
     3001 ccaaggcgct tctccacgag ccatagcgcg accgcccagg ctgcacacga tccgagcagc
     3061 aaggcggcgg cgaacgcgac aaaggtgagg tccaaatcga gcatccccgc tgcgccatga
     3121 agcacgcgac gtgccaacgc cgaggagcaa agaatcattg aggatccagc gttgcgaccc
     3181 catggggcta ggacacagat gtccgagcga cggcagcctt tgcgggatgg gttgccatcg
     3241 atggtagacg gccggagact cggctcgatg ggccgcatcg tcgtcgaaga catcgaggag
     3301 tacagctcgg tcgagcttgc caagaagtac aaatcgacgg cggggtccca accgaaagcg
     3361 gaccccaacg aggtcgaccg atgcatggag gccatcgagc gggatggctt cgccgtcatc
     3421 gagaatcgga tttcgccgga gctcgtcgcc gagatcaagg aagacctgat cccgcgcttc
     3481 gaatacgagt ccgggcgcaa caacttcgaa gggttcaaga cgcagcggct ctacgcgttc
     3541 ttcgagaagt cgctggtctg caaccccttg gtggaggact cgctgattct cggctgcctc
     3601 gaccggatct tcgagccgaa ctacctcttg tcccagcttc aggccatcaa catcctaccg
     3661 ggagaatcgc agcagccgct tcatcccgac gatgcgttct accgcgtgcc gcgtccgcga
     3721 cctccgcttg gcgcggcgac gatttgggcc atcgatgagt tcaccgaaga aaacggcgcg
     3781 acggtggtga tcccgaagag ccatctctgg gacgaccggc tacccacgga agccgatctg
     3841 aaagacttgc agccggtcgt gatgccagcg ggctcggtgg tgttcttcgt cgggacgctc
     3901 tggcatggcg gtggagccaa ccgtagcgac gccgcgcgct tgtgcgtgtc cgcgcagtac
     3961 tgcgccccct ggtgccgcac ccaggagaac tacagcctct cgctctcgcg cgagaccgtg
     4021 aagcggtgca gcgagcacat ccaacgtatg ctcggctaca gcatccatcc gcccttcatg
     4081 ggcttcgtca acggcaagca cccgaagcgg ctgctggagg actaagcgac gaagtcgtct
     4141 atttctgacg gtcccgcgtt ccgttgaacg cccacgactg catccgtggt acgagtgcga
     4201 gatggccatg tcgattccgg tttgggtgtt gctcgcgttc gcggtctgga cgctgctcgt
     4261 gctggtgttg accgtcggca tctaccgctg ggggctgatc ttcgcgggcc gggttcaagt
     4321 caaggagttc cgtgcagacc aaatcgaagg ctccgatcgg taccgccgct cgatgcgcgc
     4381 gcacttaaac tgcgtcgaga atctgccggt ctatggcgca atcgtgctcg tcatcgtcgt
     4441 cagcggtgcg cagagctc
//
LOCUS       DASRZF010000232         5541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4972177, whole genome shotgun sequence.
ACCESSION   DASRZF010000232 DASRZF010000000
VERSION     DASRZF010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 5541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5541
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4972177"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..715
                     /locus_tag="VFG22_10240"
     CDS             59..715
                     /locus_tag="VFG22_10240"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6416661.1"
                     /translation="MTRLTELTQHILGRLGSRRTFESQSYWKERYRKGGTSGAGSYGR
                     LAQFKSEVINDFVAENAVESVIEFGCGDGNQLSLYELPRYIGLDVSPEIVAQCRERFA
                     DDPSKTFHLTAPEGLQADLAMSIDVLFHLTEDEVFERYMRDLFAAATRFVIIYSSNSD
                     HNGWLQPKHVRHRKFTVWVEANEPGWTLQETIENKYPLKSNDKIESPADFFIYRKSPA
                     "
     gene            744..1886
                     /locus_tag="VFG22_10245"
     CDS             744..1886
                     /locus_tag="VFG22_10245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6416662.1"
                     /translation="MAGSFGEPKRRVAVFNTNYLLYSQTFVYEQLVNHERYVPELFCW
                     RTKNLEWFPFNPIHRANAAYGFTRYSPAFFRRFRDVRFSLVHAHFATGAVYALPYVQR
                     FELPFVVTFHGYDVPLLWNAQRFQPEFWPFLLHSKEVLERMTLGLCASTELKELLISH
                     GVPEDKLRVHRLGIDLSRFNPKSVRPEGPVRLCIVGRFVEKKGLDYAIRAYAPHASED
                     VLLDIIGDGPLAGDLHALVARLGLERFVSFLGVLRPEEVAERVSASDVLLAPSVVTRS
                     GDRESGLIVAKEAAACGVPVLATMHGGLPDIIEDGHTGFLVPERDVQRLSERLGRLIA
                     DPALRRKMGGAARLKMEREYDVRERVTELEGLYDEAIARHVRPAGG"
     gene            complement(1883..3337)
                     /locus_tag="VFG22_10250"
     CDS             complement(1883..3337)
                     /locus_tag="VFG22_10250"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HET6416663.1"
                     /translation="MTEAGKQLNRGLIWLGSAAAVGRVADLAATVTVLFFLSKEELGG
                     ATIAWAIGMVLEAVCRLGLGVAVLQAEDVTRIQLDTSWWMMTITTLGLGALAVGTGPH
                     VGPLLNEPLLGLYLIPSAVKMLFLVWGEIPLQLMNRKLQFASIAGVYSGATILSAIAR
                     VVLAATGFGAWSLLIAHALYGFFVWLMAMVASPFWPRLTMRIKEIHGLLRFGRYVTGD
                     WLTRELYQNADYLILGGLVGPAGVGVYRVAFEIAMQPAMAVVSVLNRTALPVMARLRG
                     RQRAEIFVDASGKLAIVMGAVCAVVIATADDITAIFQNGEYAAAAMPAQLLVIAGALR
                     ALFQLFPDMFTAAGAARLTFRLGMLSLIVLGSCLGVALLAIGVERAAIAMAVGWLGVY
                     PILLPVALWLGASSFALRPQDYWLKLVAPMSSAVAASLLGLRLHALLSDETPLWARIT
                     IVSVAVVATYALAQVAVGRVTRGVAARDETDPAS"
     gene            complement(3347..4594)
                     /locus_tag="VFG22_10255"
     CDS             complement(3347..4594)
                     /locus_tag="VFG22_10255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6416664.1"
                     /translation="MPRRILFSYENLLPTSQADAEVVLNTAGALAGRGYEAIVSVPAP
                     ADLSPDFEREVLDYYGIQTPLHITAVPSFTKNIALQHAYHAMHFPEHPAFADADFIYA
                     RNPIVVMRALQYGQRVLMDHYRPWGDQFPPLQPIFRRFMNHPRFLGLVVHSAYSYESY
                     RRLGVPEAKLRVVHNGFDPRRMEPVLSKQAARALLDLPQSRRIVTYAGRVNDKKGLEV
                     ILELADRMPEVTFVLVGSTERGPIEIASESKDNVRVVPWQTSSSISPYLYASDVLTIP
                     PSSVPLQRVGNTVLPLKLFLYLAAGRPIFAGASPDTAEILEHDKNAWLVSTGEASEAE
                     KQLRVLLSDTHRLDRLGRACREQAEGLTWDSRAAKIDAFVDECLARDPSVLQKGAWSA
                     SACARDSMKWLVTGISTGKWIYR"
     gene            4829..>5541
                     /locus_tag="VFG22_10260"
     CDS             4829..>5541
                     /locus_tag="VFG22_10260"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET6416665.1"
                     /translation="MKSIFRTSITPNMITLSTVVLGFSAGCAFIWGTPTAMFFGGAGV
                     WAAAILDGADGILARAKNQQSEFGRALDGSADAMVAVFTVFPAFYHIWVTYHNPYHLA
                     LMLPALVVTVIHLAVYDFYKEAYLRAIRPGQGGEGHDADEIAETLEAARSKGPITRIA
                     IKYFLVPHLERQKALVHWLNPDAWRLSQVLQSDSQTTEIYKKYNVWPMRLWALVSLAP
                     HSYLMAICAMFDRLDVYLF"
BASE COUNT         1110 a         1732 c         1642 g         1057 t
ORIGIN      
        1 accgatgggc tccattcatg ctggggcgac gccccgtgct agtgtaggct ccgcagcgat
       61 gacgcgtttg accgaactca ctcagcacat cctcggccgc ttaggcagcc ggcgcacctt
      121 cgagtcccag agctattgga aggaacgcta tcgcaagggc ggaacctcgg gggcgggctc
      181 gtatggccgt ttggcgcagt tcaaatccga agtcatcaat gacttcgtcg ccgagaacgc
      241 cgtcgaatct gtcatcgaat tcggctgcgg cgacggcaac cagctgtctc tttatgagct
      301 tccccgctac atcggactcg acgtgtcccc agagatcgtc gcccaatgcc gggagcgctt
      361 cgccgatgat ccgtccaaga cgtttcacct caccgcgccc gaaggacttc aagccgacct
      421 ggcgatgtcg atcgacgtgc tcttccacct gaccgaggac gaggtcttcg aacgctacat
      481 gcgtgacctc ttcgccgccg caacccgctt cgtcatcatc tactcatcga acagcgatca
      541 caacggctgg cttcagccga agcacgttcg ccaccggaag ttcaccgttt gggtcgaagc
      601 caacgagccc gggtggacgc tgcaagagac gatcgagaat aaatacccgc tgaagagcaa
      661 cgacaagatc gaatctccag cggacttctt catctaccgc aagagtccag cgtagggcag
      721 tgtcagcaaa gccgattgag agcgtggctg gttccttcgg ggagcccaag cgtcgggtgg
      781 cggttttcaa cacgaactac ctgctctact cgcagacctt cgtatacgag cagctcgtca
      841 atcacgagcg atatgtgcca gagctatttt gttggcgtac caaaaacctc gagtggtttc
      901 ccttcaaccc gattcaccga gcaaacgctg cgtacggttt caccaggtat agccccgcgt
      961 tctttcgccg gttccgggac gtgcgattca gcctcgtcca cgcccacttt gcaactggcg
     1021 cggtgtacgc cctcccctac gtccagcgat tcgagctgcc gttcgtcgtg acgttccacg
     1081 gctacgacgt accattgctc tggaacgcgc agcgatttca acccgagttt tggcctttct
     1141 tgctccactc gaaagaagtt ctcgagagga tgaccttggg tctgtgcgcc tcgaccgagc
     1201 tcaaggaatt gctgatctcc catggcgttc ccgaagacaa gcttcgtgtt catcgccttg
     1261 gcatcgacct cagtcggttc aatcccaaat cggtccgacc agaggggccg gttcgactct
     1321 gtatcgtggg gcgcttcgtc gagaagaagg gcctcgatta tgcgatccga gcctacgccc
     1381 cgcacgcttc agaagacgtt ctgctggaca tcatcggcga tggacctctt gccggagatc
     1441 tccacgcact cgtcgctcgc ttgggactcg aacgcttcgt ctcgtttcta ggcgtgctgc
     1501 gccccgagga ggtcgccgag cgtgtcagcg cgtcagacgt tcttctcgcc ccgagtgtgg
     1561 tgacgcgcag cggcgacaga gagtcggggc tcatcgtagc caaagaggct gctgcgtgtg
     1621 gcgttccggt gctcgcgacc atgcacggtg ggctcccgga catcattgaa gatgggcaca
     1681 cgggttttct cgtgcccgag agagacgtgc agcgactttc cgagcgcctc ggacgactga
     1741 tcgccgatcc ggcattgagg cggaaaatgg gcggcgcggc acggctcaag atggagcgtg
     1801 agtacgatgt tcgggagcgg gtgaccgagc tcgaagggct ttacgatgag gccattgcac
     1861 gccacgtgcg tcccgctggg ggctagcttg cgggatcggt ttcgtcgcgg gcggccacgc
     1921 ctctcgtcac gcgaccgact gcgacctggg caagggcgta ggttgcgacg acagcaacgc
     1981 tgacgattgt gatccgggcc caaagcggag tctcgtccga aagcagcgcg tggaggcgga
     2041 gcccaagcag cgatgcggcc acggcgctcg acataggcgc tacgagcttc agccagtagt
     2101 cctgaggcct caaggcaaag ctcgacgcgc ccagccagag ggcgaccggt aacaagatgg
     2161 gatagacccc gagccaaccc acggccatcg cgatcgcagc tcgttcgact ccgatggcga
     2221 gcagcgcaac gccgaggcaa gacccgagaa cgatgagtga aagcatcccg agccgaaacg
     2281 tgagtcgagc ggcgccggcc gccgtaaaca tgtctgggaa aagctggaag agcgcacgca
     2341 gagcccccgc gatgaccagc agttgagcgg gcatggctgc cgccgcgtat tcgccgtttt
     2401 gaaagatcgc cgtgatgtcg tccgcggttg cgatgaccac tgcgcaaacc gcgcccatga
     2461 cgattgctag cttgcctgac gcatcgacga agatctcggc ccgttgcctt ccacgaagtc
     2521 gcgccatcac aggtaaggcc gtccggttga gcacgctgac gacggccatt gcgggctgca
     2581 ttgcgatctc gaacgcaacc cgatagacgc cgacgcctgc gggccccacg agcccgccga
     2641 ggatgagata gtccgcgttc tgatagagct cacgggtgag ccagtcgccc gtgacgtacc
     2701 tgccgaatcg aagaagacca tggatttcct tgatcctcat cgtgaggcgc ggccaaaacg
     2761 gactcgcaac catcgccatg agccacacga agaagccgta gagcgcgtga gcgatgagaa
     2821 gtgaccaagc tccgaatccg gtggcagcga gcacgactcg cgcgattgcg gacaagatcg
     2881 tggcgcccga atacaccccc gcaatcgatg caaactggag ctttcgattc atgagttgaa
     2941 gggggatctc gccccaaacc aagaagagca tcttcaccgc gcttggaatc aagtagagcc
     3001 caagcagtgg ctcgtttagt agggggccca catggggtcc tgttccaacc gcgagggcgc
     3061 ccaatcccag cgtcgtgatc gtcatcatcc accacgaggt gtcgagttga atcctcgtga
     3121 cgtcctctgc ctgaaggacg gcgacgccga gtccgagccg gcacaccgcc tccagaacca
     3181 ttccgatcgc ccacgcgatc gtcgctccgc ctagctcttc cttgctgagg aagaagagca
     3241 cggtcaccgt cgcagcgaga tccgccacac gaccaacggc tgctgcagac cctagccaga
     3301 tgagacctcg attgagctgc tttccggctt cggtcattcc tatcgattat cggtaaatcc
     3361 acttaccggt cgaaataccg gtgacgagcc atttcatgct gtcccgtgcg catgcggacg
     3421 cagaccaagc gcccttctgg agcacgcttg gatccctggc caagcattcg tcgacgaatg
     3481 cgtcaatctt ggcggctcga ctatcccagg tgagcccctc ggcttgttcc cgacaggccc
     3541 gtccgagtcg atccagtcga tgcgtgtcgc tcaagagcac gcgaagctgc ttctcggcct
     3601 cggaagcctc ccccgtcgat accagccatg cgttcttatc gtgctcgagg atttctgcgg
     3661 tgtcgggact cgcccccgcg aaaattgggc gccctgcggc gaggtacagg aagagcttga
     3721 gtggcaagac ggtgttgccc actcgctgaa gcggaaccga agacggcgga atcgtgagaa
     3781 cgtccgaggc gtagaggtac ggcgagatcg aagaagaggt ttgccacggc acgacgcgca
     3841 cgttgtcctt gctctccgac gcgatctcga tgggaccgcg ctccgtcgaa ccgacgagaa
     3901 cgaacgtcac ctccggcatt cggtctgcca actcgagaat cacctcgagc cccttcttgt
     3961 cgttgacgcg accggcatag gtcacgatgc gtctactctg cggaagatcg agaagcgcgc
     4021 gcgccgcctg cttcgagagc accggttcca ttctgcgcgg atcgaaaccg ttgtgaacga
     4081 cgcgaagctt ggcctccgga accccgagtc ggcggtagga ctcatacgaa taggccgagt
     4141 gaacgaccaa accgaggaac cgtgggtggt tcatgaagcg ccgaaatatc ggttgcagcg
     4201 gagggaactg atcaccccat ggacgatagt gatccatgag aacgcgctgg ccgtactgca
     4261 gcgcgcgcat caccacgatc gggttgcgcg cgtaaatgaa atccgcatca gcgaaggcgg
     4321 ggtgctcggg aaagtgcatc gcgtggtacg cgtgctggag cgcgatgttc ttggtgaagc
     4381 tcggcaccgc agtgatgtga agcggcgtct gaatcccgta gtagtcgagc acctcccgct
     4441 cgaagtccgg agagagatcc gccggagcgg gaaccgagac aatcgcctcg tacccgcgtc
     4501 ccgctagcgc tccagccgtg ttgaggacca cttcggcatc cgcctggctc gtcggcagga
     4561 gattctcgta gctgaaaagg atccgcctcg gcacggcggg ggatctacca cgccgacgcg
     4621 ccgcgtatcg tccgcgccgg gccggcgcag tcagaggtct cttttggagc tttgtgagtg
     4681 atctcgcaga atcggcctgc ttattgcatc gggggccctc atgcgtcccg caatggctca
     4741 acttcccccg tttcgcagcg tcgtgaaacc gaacgacgtc gaagacccgg tgaatcggta
     4801 tctgcaccga accctggcgt atttgttcgt gaaatcgatc ttcaggacct ccatcacccc
     4861 gaacatgatc acgctgtcga cggtcgtgct cggcttttcg gccggttgcg cctttatctg
     4921 gggtacgccc accgcgatgt tcttcggcgg ggctggcgtt tgggcggccg caatcttgga
     4981 cggcgccgac gggattttgg cccgagcgaa gaaccagcag tccgaattcg gtcgtgcctt
     5041 agacggctcg gcagatgcca tggtcgcggt ctttaccgtc ttcccagcct tctatcatat
     5101 ctgggtaacc taccataatc cttaccatct tgccctgatg ctacccgctc tggtggtcac
     5161 ggtcattcac ctcgccgtct acgatttcta caaagaggcc tacctccgcg cgatccggcc
     5221 gggccagggc ggcgaaggcc acgatgccga tgaaatcgcc gaaaccctcg aagccgctcg
     5281 cagcaagggt ccgattacgc ggatcgcgat caagtacttc ctcgtgcccc atctggagcg
     5341 gcagaaagca ctggtccact ggctcaaccc cgacgcctgg cgccttagcc aggtgctcca
     5401 aagcgactca caaaccactg aaatctataa aaaatacaac gtctggccga tgcgcctctg
     5461 ggctctggtc tccctcgccc cgcatagcta cttgatggcc atttgcgcga tgttcgatcg
     5521 actcgatgtg tacctcttca t
//
LOCUS       DASRZF010000233         2859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3950183, whole genome shotgun sequence.
ACCESSION   DASRZF010000233 DASRZF010000000
VERSION     DASRZF010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3950183"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="VFG22_10265"
     CDS             <1..468
                     /locus_tag="VFG22_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416666.1"
                     /translation="YDQPALTEEDICAPLQPTGLDAPSRDGDLFAQLAAGCVFDADPV
                     AGRGDIEEVDGTERGREHIFTTTIDGNAPLEIERDGTTARMPMAGTETVLDDSSPSVG
                     DAMEVPAPGSPPPTDMQGALERQEPLPLLEGLDDLDGLGGDPIAGLGLEGDEP"
     gene            465..1484
                     /locus_tag="VFG22_10270"
     CDS             465..1484
                     /locus_tag="VFG22_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416667.1"
                     /translation="MNDANRWRPQKLRGGTVIQSGIDLRARQHSLDPKKRPFRALVLA
                     TRVVGEEGNLRNLSAECDLLLVRTNIQIMNVPVAQRQHGVNNVHDLWIPRASTRVLET
                     NEGIPVATSSEELNFQSTHTRRGGELPVPTSLGRADGDMVLLDFIEGDIAWPIITHAL
                     THERTNRVVRTGDGWREGSATERGNPKRDELYTHHYGAELRINELGDLLIDTVGAYSD
                     PATEDESVSSGQVRIRVKDSQKLTVAMGDDEDVLEVWKDGSQLRIDLGEGADEKLVLG
                     DTFKSYLDAELAKVNNFWSLLYNAHTHTVPMGGSSGPPASLQTETLSTMPDSALSDLA
                     KTKKT"
     gene            1485..1763
                     /locus_tag="VFG22_10275"
     CDS             1485..1763
                     /locus_tag="VFG22_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416668.1"
                     /translation="MALLAGTATTPNSLAKAIYDATVVEFGTVAGDLDTDRQRMCKAI
                     AVGIVNHIAVFADVRISTSDAGLQRDNTGGNPATLAPSTDVVLAGAVE"
     gene            1800..>2859
                     /locus_tag="VFG22_10280"
     CDS             1800..>2859
                     /locus_tag="VFG22_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416669.1"
                     /translation="MPRAPQRIESSRFVLNGPDGGAANISELRTGFKQGFIFEVLDLE
                     SGRTIPDGVHVMVLNPQRYSLTEPFQVTLTPTENDTVVAEENGIIVREITLEGTFGIT
                     EKRARGFQGAQGGGNPLSGADHFEALRNLFRRYSARKKDPLTAAKTQMIFHALRDGDH
                     FIVVPREFTTPRDAGKTRTHHEYRITLAAIAPAIQSGLVASRSGTDFFDDVTNVLRDI
                     NEGFNDMRAGIAEVTASLGQIKRKVGNINAILNGAIQLANAVGGFLAGATALINYPLQ
                     LAANVVEAVDGIADSLLLSVEGAAFGVFDENARSLRRISAAADKIIVFGDKFEATTRR
                     IEDLYSGERRLTANDIDNR"
BASE COUNT          601 a          886 c          898 g          474 t
ORIGIN      
        1 tacgaccagc cagcgctcac cgaggaagac atttgcgcac cgcttcagcc gaccggtctc
       61 gatgcgccta gccgagacgg cgatctgttc gcccagctcg cagcaggatg cgtgttcgat
      121 gccgaccctg ttgctgggcg cggtgacatc gaggaggtcg acggtaccga gcgcgggcgc
      181 gagcacatct tcactacgac aatcgatggt aatgcaccgc tcgaaataga gcgtgatggc
      241 actaccgccc gtatgccgat ggccggtacg gagactgtac tcgacgactc gtcgccttcg
      301 gtaggcgacg cgatggaagt cccagctccc ggctcgccgc cgcctaccga tatgcagggt
      361 gcattggagc ggcaggaacc gctgccgctt cttgaagggc tcgatgacct cgatggtctc
      421 ggaggtgacc ctatcgctgg attgggtctg gaaggggatg agccttgaac gacgctaacc
      481 ggtggaggcc gcagaagcta cgtggtggga cggtcatcca gtctgggatc gatctgcgag
      541 ctcgacagca ctctctcgac ccgaagaagc gccccttccg cgcgctggtg ctcgccacgc
      601 gcgtggtagg tgaggagggt aatctccgca acctctccgc cgagtgcgac ctcctcctcg
      661 tgcgcacaaa catccagatc atgaatgtcc cggtggcgca gcgtcagcac ggcgtaaaca
      721 acgtgcatga cttgtggatc cccagagcat cgacccgcgt actcgaaacc aacgagggca
      781 tccctgtcgc gacctcgagc gaagagctga atttccagag cacgcacacg cgccgtggcg
      841 gcgaactgcc ggttccgacg tcgctcggtc gcgcggacgg tgacatggtg ctcctcgact
      901 tcatcgaagg agacatcgcc tggcccatca tcacgcacgc gctgacgcac gagcgcacga
      961 accgagtcgt gcggactggc gacgggtggc gcgaaggaag cgccaccgag cgcgggaacc
     1021 cgaagcgtga cgagctatac acgcaccact acggagcaga gctgaggatc aatgagctcg
     1081 gagacctgct catcgacacg gtaggtgcct acagcgaccc ggcgaccgag gacgagagcg
     1141 tgagctcggg gcaggtccgc atccgcgtaa aggacagcca gaagctcacg gtcgcgatgg
     1201 gtgatgacga agacgtgctc gaggtgtgga aggacggatc gcagctccgt atcgacctcg
     1261 gcgagggcgc ggatgagaaa ctagtgctcg gagacacctt caagagctac ctcgatgcag
     1321 agctggcgaa ggtcaacaac ttctggagct tgctctacaa cgcgcacacg cacaccgtgc
     1381 ccatgggcgg tagctctggc ccgccagctt ccctacagac cgaaacgcta tccaccatgc
     1441 cagactccgc gctctcagac ctggcgaaaa cgaagaagac gtaaatggcg ttactcgcag
     1501 gaacagccac gactcccaac tcactggcga aggccatcta cgacgctacg gtcgtagaat
     1561 tcggcacggt cgccggagat ctggataccg accgccagcg catgtgcaag gccattgcgg
     1621 taggcatcgt gaatcacatc gcagtctttg ccgacgtgcg catttcgacg agtgatgctg
     1681 ggctccagcg tgacaacaca gggggcaatc cggcgacact cgcgccatcc acggacgtag
     1741 tccttgccgg ggctgtcgag tagcgtggct ctcttgaggg gcaagtcgta ggatgggcca
     1801 tgcctcgggc accacaacgc atagagtcgt cgcgcttcgt tctcaatggc ccagacggtg
     1861 gggccgcgaa catctccgag ctccgcacag gcttcaagca gggcttcatc ttcgaagtgc
     1921 tcgacctcga aagcggccgg acgatccccg acggtgtgca cgtgatggtg ctcaatccgc
     1981 agcgctacag cctcaccgag ccattccagg tcacgctcac gcccacggag aatgacactg
     2041 tagttgccga ggagaatggg atcatcgttc gcgagatcac gctcgagggc accttcggga
     2101 tcacagaaaa gcgcgctcgt ggcttccaag gcgcgcaggg cggcgggaac ccgctgagtg
     2161 gagccgacca cttcgaggcc ctgcgaaacc tgtttcgccg gtactccgcg cggaaaaagg
     2221 atccgctgac ggctgcgaaa acgcagatga tctttcacgc gcttcgcgat ggagaccact
     2281 tcatcgtagt tccgcgcgaa ttcaccacgc cccgagatgc ggggaagacg cgcacgcacc
     2341 acgaataccg gatcacgctc gcagccattg ctccggcgat ccaatccggg cttgtggcgt
     2401 cgcggtcggg cacggacttc ttcgacgacg tgacgaacgt cctgcgggac atcaacgagg
     2461 gcttcaacga catgcgcgcg gggatcgccg aggtcacggc gagcctgggc cagatcaagc
     2521 gcaaggtcgg gaacatcaac gccatcttga atggcgcgat tcaactagcg aatgcggtgg
     2581 gcgggttcct cgccggggcg acggctctta tcaactaccc tctacagctc gccgcgaacg
     2641 tcgtagaggc ggtggacggt attgccgact ccctgctgct atccgtcgaa ggtgcagcct
     2701 ttggagtttt cgacgagaac gcgaggagtt tgcgccgcat ctcggccgca gccgacaaga
     2761 tcatcgtatt cggggacaag ttcgaagcga cgacgcgccg tatcgaagac ctctacagcg
     2821 gtgagcggcg tcttacggcc aacgacatcg acaaccggg
//
LOCUS       DASRZF010000234         8027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_853917, whole genome shotgun sequence.
ACCESSION   DASRZF010000234 DASRZF010000000
VERSION     DASRZF010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 8027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8027
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_853917"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VFG22_10285"
     CDS             <1..385
                     /locus_tag="VFG22_10285"
                     /inference="COORDINATES: protein motif:HMM:NF013234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HET6416670.1"
                     /translation="AGNEDGAVQATYASLPAISIDHGIMEKLSSVSVVPGAFEWSDLG
                     NWTSAWELAPQDENANVLPAGGIVVDAKGNYVTAPKGKLVALVGVSDLVVVDAGDALL
                     IMPRERSQDVRQIVDALRKRDDPRR"
     gene            390..1745
                     /locus_tag="VFG22_10290"
     CDS             390..1745
                     /locus_tag="VFG22_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HET6416671.1"
                     /translation="MNPHVFRQYDIRGHAERDFDDDFVRDLGLSLGTFFSKRGLWRVA
                     VGRDCRLSSPRLHRALTAGLVETGMHVVDIGIGPTPLLYFAVHQLDLEGGVQITGSHN
                     PPDENGFKMMVGKASLYGDDITELRTAMESRSFRLTPGGSVENVDPTPAYVKKAAADI
                     VLGRRNIRFAVDAGNGAGGPLALETMGALGLDPVALLCDMDGTFPVHHADPTDPETLE
                     LLRERVLEDGLELGFAYDGDADRVGVIDARGEVIWGDKLMIILSRALLEKHPGATIIG
                     EVKCSQTMYDDIEAHGGRGVLWKTGHSLIKTKMKEEHALLAGEMSGHIFFADRYYGFD
                     DAIYATLRILEIVSQSDVPLHEMLSDVPVTFATPEIRVDCDDALKFDLVQRVVEHYRP
                     THELVDIDGARIQFDGGWALVRASNTQPVLVLRFEAESEQRLESIRAQVESVLGRFQN
                     G"
     gene            complement(1716..3485)
                     /locus_tag="VFG22_10295"
     CDS             complement(1716..3485)
                     /locus_tag="VFG22_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416672.1"
                     /translation="MSRDVGVGQLVQELERRGDRLPFEIGAFVALEACEGLLQQSVKL
                     DADLVRVTAEGSVVVAKSAESADPQEAARSLVSVLARLLVAAGPGVPPYLLDLVSESA
                     SGQSPRDLRHLHDAIEASLIPINRGASRRVLARLVRESERPPAAAVPHVDPRELDAEL
                     DELLRDPASRSLEAPRAPAPEIEEPVTERIRIPRAVARDSDTDTDTAFASFPPPVPDL
                     DTQPETLPVPPSEPAFGRASQRIPRLDAPLTPVATAAQLEEPLRNRAPEPITATIRKW
                     QPSEAPKAERSAESSSAALAASSSVTVSESVTVTAPESEIETATESVFALGSVSASES
                     IPAPQGLAGSLPPGAPQYDRPSIAEPRPPIRRGGWAAWVLAALLGVGTYAIISTGAVD
                     AWLRPASVPAIVSTDGVIEVSVTPADAQVFVFVGRGPAVASGLPVDAAHEFVVFDDGL
                     RPSRALVPKGASWTASERGSLYELAVQAETAAPSDTLDLGSPESVPAPTGTDGSGTIR
                     IITNPQGAKVYRFVGVGPTIRILAPSIHEGHEILVYHPQYETRRAVIGPSDWLSAEGG
                     YRAKLDLRLPERAASAVLEAPEN"
     gene            complement(3495..4271)
                     /gene="hisF"
                     /locus_tag="VFG22_10300"
     CDS             complement(3495..4271)
                     /gene="hisF"
                     /locus_tag="VFG22_10300"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HET6416673.1"
                     /translation="MLAKRIIPCLDVKEGRVVKGVNFVGLRDAGDPVESAQRYSAGGA
                     DEITFLDITASHENRRTLFDLVDRTASTVAVPLTVGGGVRERRDVNELLHAGADKVSI
                     NSAAVANPDFIREMAEAYGSQAIVVAVDAKRAPGQTDRWTVYTHGGRRDTGLDAIAWT
                     RQMADYGAGEILLTSMDRDGTRDGYDLVLTRAVVDAISIPVVASGGVGTLAHVHEGLA
                     AGGADAALAASIFHDGHYTVAQVKDYLLEQGVCVRPPERD"
     gene            complement(4271..4990)
                     /gene="hisA"
                     /locus_tag="VFG22_10305"
     CDS             complement(4271..4990)
                     /gene="hisA"
                     /locus_tag="VFG22_10305"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HET6416674.1"
                     /translation="MELIPAIDLLGGKVVRLHQGRYDQVTVYDDDPVSMAKRFEEQGA
                     ERLHVVDLEGARSGKPAHVSTIEGILRETQLAVQIGGGVRSEEIAAQWINAGAARVVL
                     GTMVVKAPKLARAICARFPAGVVMALDARDGKIAVEGWQEQSEQDVVELAKDVDTWNI
                     GAILFTNIHKDGTRDGPDVEGTAGLQAQVGTTVIASGGIGKLDDLRALALAGVRSAVC
                     GRALYSGAFTLSEAFAALEEL"
     gene            complement(4981..5610)
                     /gene="hisH"
                     /locus_tag="VFG22_10310"
     CDS             complement(4981..5610)
                     /gene="hisH"
                     /locus_tag="VFG22_10310"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HET6416675.1"
                     /translation="MTVGRVAIVDLGLGNLHSVAKAFERAGADAVLSSDPDVLLKADR
                     LVMPGQGAFRECAHALAGECGEAVRAYIASGRPYLGICLGMQALFESSEEAPGEKGLG
                     IFRGTVKRFRTDLRDAEGRRLKVPHIGWNQITSRHAVFEDQEWYYFVHSYYCVPDDQG
                     IVVATTDYGGPFCSAIARDNVLACQFHPEKSQHAGARLIGHFLGDISWS"
     gene            complement(5607..6200)
                     /gene="hisB"
                     /locus_tag="VFG22_10315"
     CDS             complement(5607..6200)
                     /gene="hisB"
                     /locus_tag="VFG22_10315"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HET6416676.1"
                     /translation="MGRTATIERRTTETDIRVVLDLDGRGRYEVETPIGFLTHMVEQI
                     AKHGLFDLDVRALGDTHIDGHHTTEDLAIALGQAFAVALGDKAGIRRFGSTTLPMDEA
                     CVTCALDLSGRPYFVWAVTLPKAKIGDFDSELAEVFFEGFARGAQCNLHMRQHAGDNL
                     HHVIEISFKAFARALREATERDPRVLGVPSTKGTLTE"
     gene            complement(6243..6725)
                     /locus_tag="VFG22_10320"
     CDS             complement(6243..6725)
                     /locus_tag="VFG22_10320"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HET6416677.1"
                     /translation="MPDAKEASDYYALLGIIRTANADQIRAAFHRFAREHHPDNFIGS
                     PEETERHTELYQLGSEAYRVLLNPTKRKLYDEGLEKGVLRYSEDRAKEKRRTMKPPGG
                     VVLRSSKARVFFTRAHKAIESEDWPQAKLNLQMAIQNEPDNEALKAKLEEVRERMKSG
                     "
     gene            complement(6729..>8027)
                     /locus_tag="VFG22_10325"
     CDS             complement(6729..>8027)
                     /locus_tag="VFG22_10325"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HET6416678.1"
                     /translation="NLDFALTRQQLEHMCQPLMARSFDVCEDAMKAAGLRPTQLDNVI
                     LVGGSTRIPLLQRMVAEYFGRKPLVNIDPDLVVAQGAALQAHTLSMPARKAPLGRVAL
                     KKVAQPGSEKHPRITEESFEDEVTNVGDRPPLPPPPVARAARRKATLSGQAPPPPPLQ
                     LKTPADSALDDGLLDTPTRSREEPLEQDRTRLAEAVDPLDFQPTRMADASGLLMQLGA
                     EGEPQLEEIGDEPSGLITELGDDAFGGQDSDLPTGVSPPGLPPIDLSSTAPALLRAAL
                     DEDVDTVTGAVAVTEGPQAPLLLDVTPLSLGVETVDGYCEHIIRRNSAIPAGQTKMFT
                     TARDGQEAVVVRICQGEDRQITGNQLLGEVVLDGLRPAARGKVQIAVRFMIDADGTLQ
                     VRAQDVGTGQQQQIAIKLIGELAEEQIEQLRERHEAKLGS"
BASE COUNT         1418 a         2595 c         2522 g         1492 t
ORIGIN      
        1 ggccggcaac gaagacggtg ccgtgcaagc aacctatgct tcactccccg cgatcagcat
       61 cgatcacggg atcatggaga agctcagctc ggtgagcgtg gtgcctggtg ctttcgagtg
      121 gtccgacctc ggcaactgga cctcggcctg ggagcttgcg ccgcaggatg agaacgcgaa
      181 tgtcctgccg gcaggcggaa tcgtcgtcga cgcaaagggc aactacgtca ccgcgccgaa
      241 aggaaagctg gtagctctgg tcggggtcag cgacttggtc gtggtggatg caggtgatgc
      301 gctgctgatc atgccgaggg agcgttcgca agatgtccgc cagatcgtcg acgcgcttcg
      361 caaacgggac gatcccagaa ggtgacccag tgaacccgca cgtttttcgt cagtacgaca
      421 ttcgaggaca cgccgagcgt gactttgatg acgatttcgt gcgcgacctg gggctgagcc
      481 tcggcacgtt cttctcgaag cgcggcttat ggcgcgttgc cgtggggcgt gactgccggc
      541 tgagctcacc acgcttgcat cgtgcgctga ctgcaggtct ggtcgagacc ggaatgcatg
      601 tggtcgacat cggcatcgga cctactcccc ttctttactt cgccgtgcac caactcgatc
      661 tcgagggcgg ggttcagatc acgggcagcc acaatccgcc ggacgagaac ggtttcaaga
      721 tgatggttgg caaagcctcg ctctatggag acgacatcac ggagctacgg acggccatgg
      781 agagccggag ctttcgtctg actcctggag gctcggtgga gaacgtcgat cccacgcccg
      841 cgtacgtgaa gaaagccgcc gctgacatcg tgcttggacg acgcaatatt cgattcgctg
      901 tggacgccgg aaacggcgct ggaggcccgt tggcgctcga aaccatgggg gcactgggcc
      961 tcgacccggt tgccttgctt tgcgacatgg acggcacctt tcccgtacat cacgcggacc
     1021 caaccgaccc ggagacgctc gagctgctcc gggagcgcgt gctcgaagac ggcctagagc
     1081 tcggctttgc ctacgatggg gacgctgacc gcgtgggggt catcgacgcc cgcggcgagg
     1141 tgatctgggg cgacaagttg atgatcatcc tgtcgcgcgc tctgctagag aagcatcccg
     1201 gagccacgat catcggcgag gtaaaatgct cacagacgat gtacgacgac atcgaagccc
     1261 acggtggacg gggcgtgctt tggaagacgg gacactcgct catcaagacc aagatgaagg
     1321 aggaacacgc gctcctcgca ggagagatga gcggccacat cttctttgcc gatcggtact
     1381 acggcttcga tgacgcgatt tacgccacgc ttcggattct cgagattgtc tcccagagcg
     1441 acgtcccatt gcacgaaatg ctcagcgacg tgcctgtgac cttcgccacc ccggagatcc
     1501 gagtggactg cgacgacgcc ctcaagttcg acttggttca gcgcgtggtc gagcactacc
     1561 gccccacaca cgagctcgtc gatatcgacg gcgcccgcat ccagtttgac gggggctggg
     1621 cgctcgtgcg cgcctcgaac acccagcccg ttctggtcct ccgtttcgaa gccgagtctg
     1681 agcaacggct cgaatcgatt cgagcccaag tcgagtcagt tctcgggcgc ttccaaaacg
     1741 gctgatgctg ctcgctcggg gagccgaaga tcgagctttg ctctgtaccc gccctcggcg
     1801 gagagccaat cgctcgggcc aatcaccgcg cgccgcgttt cgtactgcgg gtggtagacc
     1861 agtatctcgt gcccctcgtg gatcgaaggt gcgagaatcc gaatcgtcgg tccaaccccg
     1921 acgaaacgat ataccttcgc tccctgagga ttcgtgatga tgcggatcgt tccagaaccg
     1981 tcggtgccgg tgggagccgg aacgctttcc ggggaaccta aatcgagcgt gtcggaaggc
     2041 gccgccgtct cagcttgaac ggcaagctcg tagagcgagc ctcgttcgct cgccgtccag
     2101 ctcgcacctt tgggaactaa agctctcgat ggcctaaggc cgtcatcgaa cacgacgaac
     2161 tcgtgggcag cgtcgacggg taaacccgaa gcgaccgccg gaccacgccc cacgaacacg
     2221 aacacctggg cgtcggccgg tgtgacactc acctcgatta cgccgtcagt ggaaacgatg
     2281 gctgggaccg atgccggcct caaccaggca tccacggcgc cggtcgagat gatcgcgtag
     2341 gtcccgacac ccagaagcgc agcgagcacc caggcggccc agccgccgcg ccggatcggt
     2401 ggccgcggct cggcgatcga gggtcgatca tactgaggtg ctcctggcgg gagagagcca
     2461 gccaacccct gaggcgcggg aatcgattcg gacgcgctca cggaccccaa cgcaaagacg
     2521 gactccgtcg cggtctcgat ctctgactcc ggcgctgtta cggtcaccga ctcggacacg
     2581 gtcaccgacg agctcgccgc gagagctgcc gatgaggact cagcgcttcg ttctgctttc
     2641 ggcgcttcgg atggttgcca ttttcggatc gtggcggtga tgggctcggg cgcacggttc
     2701 cgcagtggct cctcgagttg ggcagcggtg gctaccgggg tcagcggcgc atcgagccgg
     2761 ggaatgcgtt gagaggcgcg cccaaaagca ggctccgaag gcggaaccgg aagggtctca
     2821 ggctgagtat cgaggtcggg aaccggaggc ggaaaagacg caaacgcagt gtcggtgtcg
     2881 gtgtcgctgt cccgcgctac cgctcttggg attcgaatgc gttcggtgac cggctcttcg
     2941 atttccggcg ccggggctct cggagcctcg aggcttcggg acgcgggatc gcgcaagagc
     3001 tcgtcgagct ctgcgtcgag ctcccgcgga tcgacatgcg ggaccgctgc cgcgggcgga
     3061 cgctcgcttt cacggaccag tcgagcgagc acgcgccggg atgcgccgcg gttgatcgga
     3121 atcagcgacg cctcgatcgc gtcgtgaagg tgccgaagat cgcgagggct ctgcccgctg
     3181 gcgctctcgc tcaccagatc caggagatag ggcggaacac caggacccgc ggcgacgagc
     3241 agccgggcta gcaccgacac gagcgatcgt gcggcctcct gcgggtctgc gctctccgca
     3301 gacttagcca cgaccaccga gccttcagcc gtcactcgga cgaggtcggc atcgagcttg
     3361 acggactgct ggagcagccc ttcgcaggcc tccagcgcga caaaggcgcc tatctcgaac
     3421 gggagccggt cgccgcgcct ctccagctcc tgcaccagtt ggcccacgcc gacgtctctg
     3481 ctcattgctt cccctcaatc tcgctctggc ggacggacgc acaccccttg ctcgagcaag
     3541 tagtccttga cctgggccac agtgtagtga ccgtcgtgaa agatagaagc tgcaagcgcg
     3601 gcatcggccc ctccggctgc gaggccctcg tgcacgtgcg ccagggtccc cacccccccg
     3661 gaagctacaa ccggaatgga gattgcgtcc acaactgcgc gagttaacac caaatcgtag
     3721 ccgtcccgag tgccgtcgcg gtccatgctg gtcagcaaaa tctcgccagc cccgtagtcg
     3781 gccatctgtc gcgtccaggc gatcgcatcg agcccggtgt cgcgcctgcc gccatgagta
     3841 tagaccgtcc acctgtccgt ctggcccgga gcgcgcttcg cgtcgaccgc caccacgatc
     3901 gcctgggagc cgtaggcttc ggccatctcc cggatgaagt ccgggttggc gacggcggcg
     3961 ctgttgatgg agaccttgtc ggcgcccgca tggagaagct cgttcacgtc gcgcctctcg
     4021 cgcacgccgc cgcccacggt cagcggaacc gcgacagtcg aagcggtgcg gtcgaccagg
     4081 tcgaacaagg tcctgcgatt ctcatgcgac gcggtgatat cgaggaaagt gatctcgtct
     4141 gcaccgcctg cgctgtagcg ttgcgcggac tccaccgggt cgcccgcatc gcgcagaccg
     4201 acgaagttga cgcccttgac gacgcgccct tctttgacat cgagacaggg gatgattcgc
     4261 ttcgcgagca tcagagctct tctagcgctg caaacgcttc ggacaacgtg aaggcgccag
     4321 agtagagggc gcgcccgcag accgccgagc ggacgccggc aagcgcaagc gcccgcaagt
     4381 catcgagctt acctatccct cccgaggcga ttaccgtagt tccgacctga gcttgcaggc
     4441 cagccgtgcc ctccacatcg ggcccatcac gcgtgccgtc cttgtgaatg ttggtgaaga
     4501 gaatcgcgcc gatgttccag gtgtcgacat ccttcgcgag ctccacgacg tcttgctcac
     4561 tttgctcctg ccagccctcg acggcgatct ttccatcacg cgcgtcgagc gccatgacga
     4621 cgcccgctgg gaagcgggcg cagatcgcgc gcgcgagctt cggagccttg acgaccatcg
     4681 tcccgagcac gactcgagcg gcgccagcat tgatccactg cgcggcgatt tcttcgctac
     4741 gcactcctcc gccgatttgt acggcgagct gggtctctcg aagaatccct tcgatcgtcg
     4801 agacgtgcgc gggcttcccg ctccgagcgc cctcgagatc gacgacgtgc aagcgttcgg
     4861 ccccctgctc ctcaaatcgc ttggccatgg acaccggatc gtcgtcatag accgtgacct
     4921 ggtcgtagcg gccctggtgt aaccggacca ctttgccccc gagcaaatcg atggctggaa
     4981 tcagctccac gagatatctc ccaggaagtg cccgatcagt cgtgcgccgg cgtgctggct
     5041 cttttcaggg tggaactggc aagcgaggac gttgtcccga gcgatggcag agcagaatgg
     5101 gcccccatag tcggtcgtgg ccacaacgat tccctgatcg tcgggaacgc aataatagct
     5161 gtgaacgaaa tagtaccact cctggtcttc gaatacggca tgccgactcg tgatttggtt
     5221 ccagccaatg tgaggaacct tgagtcgccg gccttctgca tccctgagat cggtcctgaa
     5281 ccgcttcacg gtcccccgga aaatcccgag gcccttctca ccaggcgcct cctcgctcga
     5341 ttcgaacaat gcttgcatac cgaggcaaat gccgagatac ggacggccgc tcgcgatgta
     5401 agcccggacc gcctccccgc actcaccggc gagcgcatgc gcgcactccc tgaatgcgcc
     5461 ctgccccggc atgaccaggc ggtctgcttt gagcaagacg tcggggtccg agctgagcac
     5521 ggcatccgcc cctgcgcgct cgaacgcctt ggctacgctg tgcaggttcc cgagaccgag
     5581 gtcgacgatg gctacccggc cgaccgtcat tcggtcaacg tacccttggt cgagggaaca
     5641 cccaggaccc gggggtcgcg ctcggtcgcc tcgcgaagcg cacgcgcgaa cgccttgaag
     5701 ctgatttcga tcacgtggtg gaggttgtcg ccggcatgtt gtcgcatgtg cagattgcac
     5761 tgtgcgccgc gcgcaaaccc ttcgaagaac acttccgcga gctccgagtc gaagtcaccg
     5821 attttggcct tcggcaaggt cactgcccag acgaagtacg gccgtcccga gagatccaag
     5881 gcacaggtca cgcaggcctc gtccatcggc aaggtggtcg aaccgaagcg ccggatccca
     5941 gccttgtcgc cgagggctac cgcgaacgcc tgaccgagcg cgatcgccaa gtcttcggtg
     6001 gtgtggtgac catctatgtg ggtgtcgccg agcgcacgca catcgagatc gaacaggccg
     6061 tgctttgcga tttgctcgac catgtgtgtg aggaagccta tcggcgtctc cacttcgtac
     6121 cggccgcgcc cgtcgagatc gagcaccaca cggatgtcgg tctcagtggt tcgacgctct
     6181 atcgtcgctg ttcgtcccat gacgcggcta ccatagcccg gaaatctctc cgggctacca
     6241 ccttagcccg acttcatccg ctcgcgaact tcttcgagct tggccttgag cgcctcgttg
     6301 tcaggctcat tttgaatggc catttgcagg ttgagcttgg cctggggcca gtcctctgat
     6361 tcgatcgcct tgtgcgcgcg cgtgaagaag acgcgggcct tgctcgaacg gagcacgaca
     6421 ccccctgggg gtttcatcgt tcggcgcttc tccttggcgc ggtcctcgga atagcggagc
     6481 actccctttt cgaggccttc gtcgtagagc ttgcgtttcg tcggattgag gagcacccgg
     6541 tatgcttcac tccccagctg gtagagctcc gtgtgccgtt cggtctcctc cggcgaccca
     6601 atgaaattgt cgggatggtg ctctcgggca aatcgatgaa acgcggcgcg gatctggtcc
     6661 gcattcgcgg tgcggatgat ccccagaagc gcgtagtagt cggaagcttc ctttgcgtct
     6721 ggcatcactt agctcccgag cttcgcctcg tgacgctccc tcaattgctc gatttgctcc
     6781 tctgccaact caccgatcaa cttgatcgca atctgctgtt gctgaccggt gcccacgtcc
     6841 tgtgccctca cctgaagagt gccatcggca tcgatcatga accgaaccgc gatctgaacc
     6901 tttccgcgcg ccgccggtct gaggccatcg aggaccacct cgccaagcag ctggttcccc
     6961 gtgatctgac gatcctctcc ctggcaaatg cggaccacga ccgcctcctg cccgtcccgc
     7021 gccgtggtga acattttggt ctgtccggca gggattgcgg agttgcgacg gatgatgtgc
     7081 tcgcagtacc cgtcgaccgt ttcgacaccg agcgagagtg gcgtcacgtc gaggagcagc
     7141 ggcgcctgag gtccttcggt cacggcgacg gcgccggtta cagtgtcgac gtcctcgtcg
     7201 agcgctgctc tgagcagggc cggagccgtg ctgctgaggt cgatcggcgg caagcctggc
     7261 ggactgacgc ccgtgggcag atcggagtcc tggccaccaa atgcatcgtc gccgagctcc
     7321 gttatcaagc ccgagggctc gtcgccgatc tcttcgagct gcggctctcc ttcggcgccg
     7381 agttgcatca agagccccga cgcatcggcc atccgcgtgg gttggaagtc gaggggatcg
     7441 acggcctcgg caagtcgagt gcgatcctgc tccaagggct cctctcgtga ccgtgtcggc
     7501 gtgtccaaca gaccatcgtc gagcgcggag tcggcaggcg tcttgagttg tagcggtggt
     7561 ggcggcggcg cttgcccgga cagcgtcgct tttctgcgcg ctgctcgtgc gaccggaggc
     7621 ggcggaagcg ggggacggtc accgacgttg gtgacctcgt cctcgaagct ctcttcggtg
     7681 atgcgcggat gcttttcgct acccggttgc gccaccttct tgagggcgac ccgaccgagc
     7741 ggtgccttcc gcgccggcat cgacagagtg tgcgcctgaa gtgcggcgcc ctgcgccacg
     7801 accaagtccg ggtcgatgtt gacgagtggc ttgcgcccga agtactcggc caccatcctc
     7861 tgaagcaacg ggatccgcgt cgagccgccc accaaaatga cgttgtcgag ctgggttgga
     7921 cgaagacccg cggctttcat cgcgtcctcg cagacgtcga agctccgcgc catgagcggt
     7981 tggcacatgt gctcgagctg ctgacgcgtg agcgcgaagt ccagatt
//
LOCUS       DASRZF010000235        14876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2918080, whole genome shotgun sequence.
ACCESSION   DASRZF010000235 DASRZF010000000
VERSION     DASRZF010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 14876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14876
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2918080"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VFG22_10330"
     CDS             complement(<1..297)
                     /locus_tag="VFG22_10330"
                     /inference="COORDINATES: protein motif:HMM:NF014464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsfS/YbeB/iojap family protein"
                     /protein_id="HET6416679.1"
                     /translation="MVKQAESAQRKSSPSPASSNEQVVGRELALKVAEAALDKKAGHI
                     EIIDVGGKVDYTDYVVVMSGRSDRQVSAIARGIEEDLKLKYGSRCSGVEGLPQGN"
     gene            complement(314..1579)
                     /locus_tag="VFG22_10335"
     CDS             complement(314..1579)
                     /locus_tag="VFG22_10335"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HET6416680.1"
                     /translation="MSDDLKELVTGLAQRAKKASRVLATASTEQKNAVLRRAARALRG
                     EAGDRVLQANVKDMAAAEQMGLSKAMLDRLELDRKRLDSVADGLEQVAELPDPVGTLV
                     EERVLSNGLRVGKMRAPLGLIGIIYESRPNVTADAASLCLKSGNAVLLRGGKEAFHSN
                     TAIAEIFTQALEDEGLPAEAVTLLPTTDREATLVMIGLDGILDMVIPRGGEGLIRFVA
                     EHARVPVIQHYKGVCHVFVDRTADPEMALEIALNAKVQRPGVCNSMETLLVDANVAPT
                     FLPKLGAAMRAEGVEIHADDRAAKHLNGVKKATEADWDTEYLDLILNVGVVDGIDGAI
                     EHVAKHGSNHTEAIVTADSANADRWTREVDASLVLVNASTRFNDGYALGLGAEIGIST
                     TKLHAYGPMGLAELCTLKWVGRGEGQIRQ"
     gene            complement(1626..2735)
                     /gene="proB"
                     /locus_tag="VFG22_10340"
     CDS             complement(1626..2735)
                     /gene="proB"
                     /locus_tag="VFG22_10340"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HET6416681.1"
                     /translation="MDDQDIRTALSDCKRIVVKIGSRALCADGGRFEQVAKQVAAQVT
                     AGRRLVLVSSGAVALGRERLGLTERPKKIALLQAAAATGQSLLMRAYEDAFEPLGIHV
                     AQVLLTHDAVTDRDRYLHARQAIDELLKLGVVPIINENDTVSIDELRFGDNDQLAAMV
                     CTLVGADLLILLSDVVGILDDKNERISLVHDVSEIDRFIRPPEGDVGLGGMQSKVEAA
                     RRATLHGLPVVIGPAGSSNFLDALMRGDDVGTLLLPHGSPLASRKHWIAYTLKPRGRI
                     VVDAGACRALEKRNSSLLPAGIVSVTGSFKVGDSVSIVSESGKELARGLARYSAEDVK
                     LLAGGHSQQIAERIGNHTGDEVVHRDDIVLTGRGA"
     gene            2826..3728
                     /locus_tag="VFG22_10345"
     CDS             2826..3728
                     /locus_tag="VFG22_10345"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HET6416682.1"
                     /translation="MFAGEVARWFVWLFAFAWGAVWGSFFNVAIYRWPRGMSVVTPPS
                     HCPGCGQAIRVWNNVPILGWLFLRGKASCCGSAISPRYPFVELLTGVLSVVIAEIWVV
                     RAAPETLFLHASIEAILYFVFAGGLLIATFVDLEWMEIPDEVSLPCAALGLLSAPFRA
                     EPGLWSAAVGAGAAYLSVQLIFVWGYERIAGRRGMGEGDAKLLLMIGAFLGWKGALFS
                     VVAGSMQGLLFAAFALLTGRKLTPEIEERLIDGEIIEAPEEPDTSRVGQLKLPFGPFL
                     ALGALEYLLAGDAILSWYFGLLQR"
     gene            complement(3731..4036)
                     /locus_tag="VFG22_10350"
     CDS             complement(3731..4036)
                     /locus_tag="VFG22_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET6416683.1"
                     /translation="MSKRLFCGGLAWATTTEDLQDAFAPFGEIADAKVVSDRETGRSR
                     GFGFVTFTTEEAASAARDQMDGASLGGRTIRVDLAREREGGGGGGGGGGGGRGGYRR"
     gene            complement(4088..4453)
                     /locus_tag="VFG22_10355"
     CDS             complement(4088..4453)
                     /locus_tag="VFG22_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416684.1"
                     /translation="MSKSPLSIVKERFGDDPKKAKEKLVAAVKKLAGKDVWLDRLNEG
                     KGLDHVSNKKLLHLEQVFTAVSKEVGSRDKLISEIANLQGRSKDVDYKARLAEESTPA
                     LWDRFQAVQSNSSGKPGSN"
     gene            4620..5294
                     /locus_tag="VFG22_10360"
     CDS             4620..5294
                     /locus_tag="VFG22_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416685.1"
                     /translation="MNNPKLAQVQAVSGLLFSVFALVHLVNIALAILGADLYDGFQSS
                     ARGIYQWPPLELALVATLIVHIVSGVLRMRGRRGSKAKPPLRLRLHRYAAYYLALFVF
                     GHMAATRLPALLADAPPFFGGVSFSMHFMPWFFYPYYGLLGIAGLYHLFYGVPVALGV
                     LGVAAPRSIRRGPGFWLPVGTGALVIVIALLGFGGLLYEIDDPFDNDFARAYENAIDR
                     LSLDPQ"
     gene            5297..5899
                     /locus_tag="VFG22_10365"
     CDS             5297..5899
                     /locus_tag="VFG22_10365"
                     /inference="COORDINATES: protein motif:HMM:NF037007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6416686.1"
                     /translation="MGRPKDADPERTRATILDAAEDEFAEHGFHGTRMTAIANRAGVT
                     HGLLHYYFDSKDHLYEQVVGRLFDRHLRLFQGLAADGNKPGAREVVLESFDLFWEYPN
                     QVRIMLWEMASGDDRVERAMRPFYEVMASTLAGLAEGAPADCESGRDPRDVYVSILGA
                     LVVYFFRDPAIKSLFGENRFTEEDRKRRRAHMGALLDALL"
     gene            5951..9583
                     /gene="dnaE"
                     /locus_tag="VFG22_10370"
     CDS             5951..9583
                     /gene="dnaE"
                     /locus_tag="VFG22_10370"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HET6416687.1"
                     /translation="MVAEFVHLHVHSEFSMLDGAIRIPQLVEHVGRMEMPAVALTDSG
                     NMFGAVQLVRACSKTEVKPILGCEVNFTSGDRRDPNNHELHHLLLLASSQEGYQNLVR
                     LVSAGWVDGMAVYDQPRIDFEVLRRHSKGVVGLSACMGGYLAQEILNKGPAVGRAALA
                     QLRDSFDPGYFFVELQDHGFPENRPLNEVLGRLAGELELPLVATNDCHYLERQSAHAQ
                     LVLQCIGAGRSVREMEAVHHHSSELYLKTPEQMVNLFAHVPDAAKNTLLVAEMCAGRA
                     TPLAEPKLPRFTVPGGASEEDFLRALSVAGLGRRMVEMRGRGEVFEEQRYHSRLELEL
                     GVILKMGFAGYFLIVQDFINWAKDNGVPVGPGRGSGAGSLVAWALRITDLNPITHGLL
                     FERFLNPERVSMPDFDIDFCMDQRDRVIQYVREKYGQASVGQIATFHQLKSRSVVRDV
                     GRVMGMTPGEAGLLATMIPEPVGGKSVSIRDALEQEARLKAAYEQDKEAHELLDTAQE
                     LENLTRHAGMHAAGVVISEGPIWDHVPVFCPEKDVYVTQFHKDDVEAAGLVKFDFLGL
                     RTLTVIDIATKLIVRRPDRQDEPFRIDEIPMDDDATYALLQSGETTNVFQLESSGMQN
                     LFKQLRPDRFDDIVAAVALYRPGPMGAQMDKDFVHRKHGRQRVQYPHPSLEGILEETR
                     GVIVYQEQVMQIAQEMAGYSLGGADLLRRAMGKKKASEMETQKAIFIKGATERGHTQE
                     KAEEVFDLMAYFAGYGFNKSHSAAYALITYQTAYLKSHYPVEFTCATLTADKEKIDKV
                     VRTVAEARSMGITVLPPDVNESEIEFTVVYNPDDAQDVARLRHKPVCYNGEVHDPMGP
                     KIRFGLGAIKGVGAGAVEAILEARHGTPEVEGGEALERPFVDLFDFCARVDLRRVNKS
                     VIEALVQCGAFDTVHHAIDVHRAQSFVAIEQAIERGKRIQSERSSGQESLFGLMGGED
                     DTQAYQHPGGSFPVLAPWDSRDQLSREKGTLGFYVSGHPLDRYRGELLRFCDATTESA
                     AQLGNNRQITVGGTVEGYRERRTKMGHTMAFFHIEDAYGRIEVIVRPKPLEKGGLREA
                     LKAGEPILLTGRVKHEQDRNDESAAPEAKILLEEATLLSEALQARTTSITVRLSVETI
                     DRSKLDSLRSMLEKYPGPCPVTLELNSPGDWRVNLAETGLFVDPSDALLTGLERLFGT
                     KVCELH"
     gene            9583..10305
                     /gene="mutH"
                     /locus_tag="VFG22_10375"
     CDS             9583..10305
                     /gene="mutH"
                     /locus_tag="VFG22_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HET6416688.1"
                     /translation="MGAGSDPSLEGRVEPPGSVDELLSRAWRVAGLNLAELAAQVGIQ
                     VPDEAVRAKGIAGQILEKVLGATAASKAEPDFVGLGIELKTIPLDASGKPKESTFVCS
                     ISLSAMADTEWEQSTVLKKLSKVLFVPVEAASETPLRDRRVGRAYLWTPSHQERRLLQ
                     TDWERLAEMIAAGDVERITGHLGEVLQVRPKAPHGRSRRRAPDEEGAVQWTMPRGFYL
                     RPSFTGSVLARLRAEELDGARR"
     gene            complement(10342..12492)
                     /locus_tag="VFG22_10380"
     CDS             complement(10342..12492)
                     /locus_tag="VFG22_10380"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HET6416689.1"
                     /translation="MNMLNGIARSGLVLFTLACVLTACSSDGSATDTVSSESGRVRVM
                     TDPFSLRVEDNTGKLVLTTLARTEDAGDTLYAPLGFVTGDDPELRYQVLAGFGGQPDP
                     NPTNPVPPKRHSATEVRSIERSGDALLVVLDTDDPDGRTMTLRIAPDETGALAVDAEV
                     SDAAGVSAVFASFASPPDEAFHGFGGRRESTDLRGLAFQNWVQDYRFPDLTTGYYYAQ
                     PQFISSEGYGLFIDTDALASWRLASDLDDAWRLIAAGDRLSLMIAPGDGARATALLTE
                     ATGRHRVAPAWSLGPTLSRTVITFPGVETAETYRDRVLDDLAMIESRDLGVEAYAFEG
                     WARLEPDFVSEVIQRLAREGIRAILYIRPFVAEDLVLTEPSGRFAEAVDQGYVATTAA
                     GAAYIYPNSFDGGEAAVIDFTNPEARSWWKGLVQQMLDLGADGFMNDFGEQVIADMHF
                     SDGSTGATMHNAYPRLQHEATREAIDEYLVEHPEREIFIFVRAGYSGRAGSAAFESAT
                     FPGDEAVDWSEENGLPSIVPDMLNRAVGGAYGFSTDIGGYADWTGIPGTSKELFVRWA
                     QAAVFTTHFRVHGSAFNGVRMPWFFEPGDPEPKAEALWKAAADLHVRARPLILQLWQE
                     AVDTGMPITRPMWLHDPSAEGNPHNDDQWMLGPDLLIAPVLAKGATQREVWLPEGCWQ
                     LHGEGEELSGRANVTVDAPLDVLPWFTRCGASPL"
     gene            complement(12619..13785)
                     /locus_tag="VFG22_10385"
     CDS             complement(12619..13785)
                     /locus_tag="VFG22_10385"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET6416690.1"
                     /translation="MGAAGEAPTIDYDPIYDPFNPEVIANPYPFYERLRDEHPVYHNE
                     RMNFVVLSRYDDIFHALRRPQIFSSAQGLTPAKDEISKLGIPPTFIMMDPPDHTRLRR
                     LITKAFTPDTVKELEPTIRQFVVERVDAMKQKAADGVAVDLIEEIASPLPTMVVANIL
                     AVPAEDREQFDPWSDGITSASADFDIDLKQAAGAVQGLVTYFGKMCQARRANPTDDMI
                     SKLTQAEIDGDTLSDWDILGFCFVMIAGWNDTTTNPIGNSMRWLDDYPDQRREMIQDP
                     STIPNAIEELLRIESVVFEPSGFEVDAQPGETIMDITDAHPAAAVPYSCRAATCGTCR
                     VDVLEGGEALAAADEFELEVLEIFGDTPDKVRLCCQAKLVGGTVGQVTLRVRED"
     gene            complement(13897..>14876)
                     /locus_tag="VFG22_10390"
     CDS             complement(13897..>14876)
                     /locus_tag="VFG22_10390"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET6416691.1"
                     /translation="ETRFEPCDLTDANAVLRLVARVKPNVVVHCAAVVAPIAYAEPER
                     AEAVNLHGTQHLIDATRKHAPDAFFVFVSSYAAFGPCGPRDPNRQSTDPCWPDDNYGL
                     QKLTAESWLRFSGLRQCSLRLGAVMDLRDMMPRHPSYRPFVFMVSLDQPEHGIAARDA
                     ARAIAAAAVVQPDGHVLLIGGDDSWKLPAGRLRKDMFGAIGLPVPPERAFRPGPDPRS
                     NAGWFYECWMDGKQSEQLLRFQRIGHDAFMEELRKRHRKQRVALAPLKPFVSWALGAA
                     SPYTGRNAIQPGPTIWDDICRVYEIPEGVARNRSSVPPPPPPFGVGSGG"
BASE COUNT         2611 a         4650 c         4789 g         2826 t
ORIGIN      
        1 attgccctgc ggaagacctt cgacacccga gcaacgggaa ccgtacttga gcttgagatc
       61 ctcttcgatg ccgcgtgcga tggccgacac ttgacggtcg cttcgcccgc tcatcactac
      121 gacgtagtcg gtgtagtcga ccttgcctcc gacatcgatg atctcgatgt ggcccgcctt
      181 cttgtcgagc gctgcctcgg cgaccttcaa tgccaactcg cggccgacca cttgctcgtt
      241 gctcgatgcc gggcttgggc tcgattttcg ctgtgcactt tctgcttgtt tgaccaacta
      301 gcttcctcct tcactactga cgaatttggc cctcaccgcg gccaacccat ttgagagtac
      361 agagctcagc caagcccatt ggaccatacg cgtgaagctt tgttgtcgaa attccgatct
      421 cggctcctag gccgagtgcg tagccgtcgt tgaagcgcgt ggaggcgttc acgagaacca
      481 ggctcgcgtc gacctcgcgc gtccaacgat cggcgttggc cgagtctgcg gtgacgatcg
      541 cttcggtgtg attcgagccg tgcttcgcca cgtgctcgat cgcgccgtcg atcccatcga
      601 cgacgcccac gttgaggatc aaatcgagat actcggtgtc ccagtcggcc tcggtcgcct
      661 ttttgacgcc gttgaggtgc ttcgccgcgc gatcgtcggc gtgaatctcg acgccctcgg
      721 cacgcatcgc ggcgccaagc ttcggcagga acgtcggtgc gacattggcg tcgaccagga
      781 gggtttccat cgagttacaa acacccgggc gctgcacctt ggcgttcaag gcgatctcca
      841 atgccatttc ggggtcggcg gtgcgatcca caaagacgtg acacacgcct ttgtagtgct
      901 ggatgactgg gactcttgcg tgctcggcga cgaaccgaat caatccttct ccacctctcg
      961 gtatgaccat gtcgagaata ccatcaagac cgatcatcac caaagtcgct tctcgatcgg
     1021 tggtgggcaa gagtgtgaca gcttcggccg ggaggccttc gtcttcgagc gcctgcgtga
     1081 agatctcggc gatcgccgtg ttgctgtgaa atgcctcctt gccgccgcgg aggaggaccg
     1141 cgttgccgct tttcaggcag agcgaagccg cgtcggcggt gacgtttgga cgggattcat
     1201 agatgattcc gatcaagccc agcggtgcgc gcattttgcc gacgcgaagt ccgttgctga
     1261 ggacacgttc ctcgacgagg gtgccgacgg gatcgggaag ctcggcgacc tgctcgagcc
     1321 cgtccgcgac cgagtctagg cgtttgcgat cgagctctag gcgatcgagc atggctttcg
     1381 agaggcccat ctgctcggcc gccgccatgt ccttcacgtt ggcttggagc acacggtcac
     1441 cggcctcgcc gcgcaaggct cgagcggcgc gacgcaagac cgcgttcttt tgctcggtgc
     1501 tcgccgtcgc tagaacccgc gaggctttct tggctcgctg ggccaggccc gtgacgagct
     1561 ctttgaggtc atcgctcata gcccgtgacg gtagcacctt ttgcgcggag ggggagccgt
     1621 gcccctcagg ccccacggcc ggtgagaacg atgtcgtcgc gatgcacgac ttcgtcgccc
     1681 gtgtggttgc cgattcgctc tgcgatctgc tgactgtgac cgcctgcgag gagtttcacg
     1741 tcctcggcgc tatagcgggc caggcccctc gcgagctcct tgccagactc gctcacgatc
     1801 gacaccgagt caccgacctt gaacgagccc gtgaccgaaa cgatgcccgc tggcagcaag
     1861 ctggagttcc gcttctcgag ggctcgacat gcgcccgcat cgacgacgat gcggccccgc
     1921 ggcttgagcg tgtacgcgat ccagtgcttt cgactggcca gcggtgagcc gtgcggcaag
     1981 agcaaggttc ccacgtcgtc gccgcgcatg agggcgtcga gaaaattcga cgaaccagcc
     2041 gggccgatga ccaccggaag accgtgcagc gtggcacgcc gtgcggcttc gactttggac
     2101 tgcatcccgc cgagcccgac atctccctcg ggaggccgga tgaagcgatc gatttccgaa
     2161 acatcgtgca cgagcgagat gcgctcgttt ttgtcatcga ggattcccac gacgtcgctc
     2221 agcaagatca gcagatcggc tccgaccagc gtgcagacca tggccgccag ctgatcgttg
     2281 tcgccaaagc ggagctcgtc gattgaaacc gtgtcgttct cgttgatgat cgggaccacc
     2341 cccaacttca agagctcgtc gatcgcttgc cgcgcgtgca agtatcgatc gcgatcggtg
     2401 accgcgtcgt gcgtcagcag aacctgcgcg acgtgaatcc ccaaaggctc gaaggcgtct
     2461 tcgtacgcgc gcatgagcaa ggactggccg gtggcggccg cagcttgaag gagcgcgatc
     2521 tttttcgggc gctcggtgag cccgagacgc tctctgccca aggcgaccgc acccgacgag
     2581 acgagcacga gtctgcgccc ggccgtcacc tgcgcggcga cttgtttggc gacctgctcg
     2641 aaccgccccc catcggcgca aagagcgcgg ctgccgattt tgaccacgat tcgcttgcag
     2701 tcgctgagcg cggttctgat gtcctggtcg tccatgggat gcccactgaa tcgcattctg
     2761 acggcgaccc tgcaaccggc gagcgaaaaa ggggacagtc gccttttcgc tggtagcatt
     2821 gtggaatgtt tgcgggggag gtagcgcgct ggttcgtctg gctttttgct tttgcctggg
     2881 gggcggtctg gggaagcttc ttcaacgttg cgatctaccg ctggcctcgg gggatgtccg
     2941 tggtcacgcc gccgagccac tgtccgggct gcggccaagc gattcgcgtt tggaacaacg
     3001 tgccgatcct cggctggctg ttcttgcgag gcaaagcttc ctgctgcggc tccgcgatct
     3061 cgccccgcta ccccttcgtc gagctgctca ccggggttct atcggtcgtg atcgcggaga
     3121 tctgggtcgt gcgcgccgcg ccggaaacct tattccttca cgccagcatc gaagccatcc
     3181 tctacttcgt cttcgcgggc ggtctgctga tcgccacctt cgtcgatctg gaatggatgg
     3241 agatccccga cgaggtgagt cttccctgtg cggccttggg cttgttgagc gctccgttta
     3301 gggcggagcc cggactttgg tccgccgcgg tgggagctgg ggcagcgtac ctctcagtgc
     3361 aattgatctt cgtctggggc tacgagcgaa tcgccggccg gcgcggcatg ggcgaagggg
     3421 atgcgaagtt actcctgatg atcggcgcat ttctcggatg gaaaggtgcg ctcttcagcg
     3481 tcgtggctgg gtccatgcag gggctgctgt tcgcagcgtt tgcattgctg accggacgca
     3541 agctcacccc cgagatcgag gaacgcctca tcgacggcga gattatcgaa gcgcccgagg
     3601 aacccgacac gagtcgcgtc gggcagctga agcttccgtt tggtccgttt ctcgcgctcg
     3661 gcgcgctaga gtaccttctc gcaggcgacg cgatactcag ctggtacttc ggactgctgc
     3721 agcgatgagc ctagcgacgg tagccgccgc ggccgccacc accaccgccg ccaccaccgc
     3781 cgccaccctc acgctcacgc gcaaggtcga cgcggatggt gcggccgccg agcgaggcgc
     3841 cgtccatctg atcgcgtgct gctgaggccg cctcttctgt cgtgaaggtc acgaatccaa
     3901 agccgcgcga gcggccggtc tcacggtcgc tgacgacctt ggcatcggca atctcaccga
     3961 agggcgcgaa cgcatcctgg aggtcttcag tggtcgtggc ccaggcgagc ccaccgcaga
     4021 acagtctctt actcatggtc aactctttca ctgtgcaacc gacacggcgc catgccgtgg
     4081 cggtgcttca attcgatccg ggcttcccgg aagagttgga ttgtaccgct tggaaccgat
     4141 cccacaaggc cggcgttgac tcctcggcca agcgtgcctt gtagtccacg tccttggacc
     4201 gaccctgaag gttggcgatt tcgctgatta acttgtcgcg agaaccgacc tctttgctga
     4261 ccgcggtgaa gacctgctcg aggtgaagca gcttcttgtt ggacacgtga tccagcccct
     4321 tgccctcgtt caagcggtcg agccagacgt ccttgccggc aagcttcttt acggctgcga
     4381 ccagtttctc tttcgctttc ttcggatcgt caccgaagcg ctcttttaca atcgacaggg
     4441 gggacttgct catcacatgc accttggccc caaaaaatgc cgggttttct ggcctgggga
     4501 gcgcccggag tagcacggag accctcccac gcaagccgcc aacccaatct tcccaggttg
     4561 agattggagg gggcgagact ggcttgacag gtattaacac cgctgttact aacagtgctg
     4621 tgaataatcc aaagctggct caggttcagg cggtttcggg gctcttgttc tccgtcttcg
     4681 ccctagtcca cctcgtgaac atcgccctcg ccatcctggg agccgacctc tacgacggct
     4741 ttcagagctc ggcccggggc atctaccagt ggccgccgct cgagctcgcc ctcgtcgcga
     4801 ccctgatcgt ccacatcgtg tcaggcgtcc tcaggatgcg tggcaggcgc gggtccaagg
     4861 ccaagccccc gcttcgattg cggcttcatc gctacgcggc gtactatctc gcgctcttcg
     4921 tgttcggcca catggcggcg actcgccttc ccgcgctgct cgcggatgca ccaccgttct
     4981 tcggaggggt ttcgttctcc atgcacttca tgccctggtt cttctacccg tactacgggc
     5041 tcctcggcat cgcgggtctc taccacctct tctacggggt cccggtcgcg ctcggcgtgc
     5101 ttggggtcgc ggcaccgcga tcgattcgcc gcggccctgg gttctggctg ccggtcggca
     5161 ccggcgcgct cgtgatcgtg atcgccctgc tgggctttgg cggcctactc tatgaaatcg
     5221 acgatccttt cgacaacgac ttcgcgcgcg cctacgagaa cgcgatcgat cgactttcgc
     5281 tggacccgca gtaacgatgg gacgacccaa ggacgccgat ccggaacgaa cccgagcgac
     5341 catactcgac gccgccgaag acgagttcgc cgagcacggc tttcatggga cgcgcatgac
     5401 ggccatcgcc aatcgcgcag gcgtcactca cgggctcttg cactactatt tcgattcgaa
     5461 agatcactta tacgagcagg tcgtcggccg cctcttcgat cgtcacctcc gcttgtttca
     5521 aggtctggcc gccgacggaa acaagcccgg cgcgcgtgag gtcgtgctcg agagctttga
     5581 tctgttctgg gaatatccca atcaagtccg aatcatgctc tgggagatgg cctcgggcga
     5641 cgatcgcgtc gaacgcgcga tgcggccttt ctatgaagtg atggccagta cgctcgccgg
     5701 ccttgctgag ggcgcgcccg ccgactgcga atcagggagg gacccgcgcg acgtatatgt
     5761 gtcgatactc ggcgccctgg tcgtctactt cttccgtgat ccagcgatca agagcctctt
     5821 cggcgaaaat cggttcaccg aggaggaccg aaagcgacgg cgcgcccaca tgggcgccct
     5881 actcgacgcg ctgctttgaa cgaaagtccg gtggattcgg tccagcccag gcagtagagt
     5941 cagcccgcac atggttgccg aattcgttca cctgcacgtt cacagcgagt tctcgatgct
     6001 ggacggggcc attcggattc cgcagctcgt cgagcacgtg ggcaggatgg agatgcccgc
     6061 ggtcgcgctc accgacagcg gcaacatgtt cggcgccgtc cagctcgttc gggcgtgcag
     6121 caagaccgag gtgaaaccca ttctcggttg cgaggtcaat ttcacatcgg gcgaccggcg
     6181 cgatccgaac aatcacgagc ttcatcacct gttgctgcta gcgagctcac aagaaggcta
     6241 tcagaacctc gtgcgcctgg tgagcgccgg ctgggtagac gggatggccg tctatgacca
     6301 gccgcgcatc gacttcgagg ttctccgacg acacagcaaa ggagtcgtcg gcctgagtgc
     6361 gtgcatgggc ggctacctgg cccaggagat cttgaacaag ggacctgcgg tcgggcgcgc
     6421 tgccctcgcg cagcttcggg actcgttcga tccggggtac tttttcgtcg agctgcagga
     6481 ccacggtttt cccgagaatc gaccgctcaa cgaagttctg ggcaggcttg cgggggagct
     6541 cgagctgccg ctcgtcgcga caaacgactg tcactacctc gagaggcagt ctgcgcacgc
     6601 tcagctcgtt cttcaatgca tcggcgccgg gcgtagcgtg cgtgaaatgg aagcggtgca
     6661 tcaccattcc tcggagctct atctgaagac gcccgagcaa atggtgaacc tcttcgcgca
     6721 cgtgcccgat gcggcaaaga acactctctt ggtcgccgag atgtgcgctg gccgagcaac
     6781 gccgctcgcc gaaccgaagc taccgcgatt cacagtgccg ggcggcgcaa gcgaagaaga
     6841 tttcctgcgg gcgctctcgg ttgccggttt agggcgccgc atggtggaga tgcgcggccg
     6901 cggtgaggtc ttcgaagagc agcggtacca cagtcgtctc gagctcgagc tcggtgtgat
     6961 cttgaagatg ggctttgccg gctacttcct catcgttcag gacttcatca actgggcaaa
     7021 ggacaacgga gtaccggtgg gtcctggacg cggatccgga gctgggtcgc tcgtcgcttg
     7081 ggcgcttcgc atcaccgact tgaatccaat cacgcatggg ctgctgttcg agcgattcct
     7141 caaccccgaa cgcgtttcga tgcccgactt cgacatcgac ttttgcatgg accagcgcga
     7201 tcgcgtcatt cagtacgtac gcgagaagta cgggcaggcg agcgtcggac agatcgccac
     7261 cttccatcag ctcaagagca gaagcgtcgt tcgcgacgtg gggcgcgtca tgggcatgac
     7321 gcccggcgaa gcgggcttgc tcgccacgat gattcccgag ccggtcggcg gtaagagcgt
     7381 gtcgatccgc gatgcgctcg agcaagaggc gcgtctcaaa gcggcctacg agcaagacaa
     7441 ggaggcgcac gagctcctcg atacggcgca agagctcgaa aacctcacgc gccacgcggg
     7501 catgcacgcc gccggcgtgg tcatcagcga gggcccgatt tgggaccatg tgccggtgtt
     7561 ttgtcccgag aaggacgttt acgtcacgca gttccacaag gacgacgtgg aggccgccgg
     7621 actggtcaag ttcgacttcc tcgggcttcg tacgctcacg gtcatcgaca tcgcgaccaa
     7681 gctgatcgtc cggcgcccgg accgccagga cgagcctttc cgcatcgacg agatcccgat
     7741 ggacgatgac gcaacgtacg cgcttctcca atccggagag acgaccaacg tcttccagct
     7801 cgagtcgagc ggcatgcaga acttgttcaa gcaacttcgc cccgaccgat tcgacgacat
     7861 cgtcgccgcg gtcgcgctct atcgacccgg cccgatgggc gcgcagatgg acaaggactt
     7921 cgtgcatcgc aagcacggcc ggcagcgggt ccagtatccg catccctcgc tcgagggcat
     7981 tctcgaagag acgcgcggcg tgatcgtcta ccaggagcag gtcatgcaga tcgctcagga
     8041 gatggcgggc tactcgctcg ggggcgcgga cctcttgcgg cgcgcgatgg gcaagaagaa
     8101 ggcctccgag atggagacgc aaaaggccat cttcatcaag ggcgcgaccg agcgcgggca
     8161 cacgcaggaa aaggccgagg aagtcttcga cttgatggcg tacttcgccg gctatggttt
     8221 caacaagagc cactcggccg cgtatgcgct catcacttac cagacggcgt atctgaagtc
     8281 gcactacccg gtcgagttca cctgcgccac gctgaccgcc gacaaggaaa agatcgacaa
     8341 ggtggttcgc acggtcgccg aagcgcgatc gatggggatc accgtactgc cccccgatgt
     8401 gaacgaatcc gagatcgagt tcaccgtcgt ctacaacccg gacgacgcgc aagacgtagc
     8461 caggcttcgg cacaagccgg tttgttacaa cggtgaggtg cacgacccga tggggcccaa
     8521 gatccgtttt ggcctgggcg caatcaaggg ggtcggcgcc ggcgcggtag aagcgatcct
     8581 cgaagcgcgg cacgggactc ccgaggtaga aggcggagaa gcgctcgagc ggccgttcgt
     8641 ggatctgttc gatttttgcg cgagggtgga tctgcgtcgg gtgaacaaaa gcgtgatcga
     8701 ggcgctggtt cagtgcggtg cattcgacac ggttcaccac gccatcgacg tgcaccgggc
     8761 tcaatcgttc gtggcgatcg agcaagcgat cgagcgaggg aagcgcatcc agtcggagcg
     8821 ttcgagcggc caggaaagcc tctttggttt gatgggcggc gaggacgaca cgcaggcgta
     8881 ccagcaccca gggggcagct tcccagtgct cgcgccctgg gattcgcggg accagctttc
     8941 gcgggagaag ggcacgctcg ggttttacgt gtcgggacat ccactcgacc ggtatcgcgg
     9001 cgagctcttg cgcttctgcg atgcgacgac ggagagcgct gcgcagctcg gcaacaaccg
     9061 gcagatcacc gtgggcggca cggtcgaagg ataccgggaa cgccgtacga agatggggca
     9121 cacgatggcg ttctttcaca tcgaggacgc gtacggccga atcgaggtga tcgtgcggcc
     9181 gaagcctctc gaaaagggcg gcctccgcga agcgctcaag gcgggtgagc ccatcttgct
     9241 cacgggccgt gtcaagcacg agcaggaccg caacgatgaa agcgcggcgc ccgaagcgaa
     9301 gatcttgctc gaggaggcaa cgctcctaag cgaggcgctt caagctcgga ccacgtcgat
     9361 cacggtccga ctcagcgtcg aaaccatcga ccggagcaag ctcgactcgc tgcgctcgat
     9421 gctcgagaag tatccggggc cctgccctgt gaccctggag ctcaactctc caggggactg
     9481 gcgcgtgaac cttgccgaga ccgggctctt cgtcgatccg agcgacgcgc tcttgaccgg
     9541 cctcgaacgg ctattcggca ccaaggtctg cgagcttcac tgatgggagc ggggtcagac
     9601 ccctcgctcg aagggcgggt cgaacctccg ggctccgtgg acgagctctt gtcccgtgcc
     9661 tggcgggtcg ccgggctgaa cctcgcggag ctcgctgcgc aagtcgggat ccaagttccc
     9721 gatgaagccg tgcgtgcaaa ggggattgcg ggacagattc tcgaaaaggt cttgggcgcg
     9781 acggcggctt cgaaggcgga accggatttc gtaggactcg ggatcgagct caagacgatt
     9841 ccactcgacg cgagtggcaa gccgaaggag tcgacgttcg tatgttcgat ttcgctttcg
     9901 gcgatggccg acacggaatg ggagcagagc acggttctga agaagctatc caaagtcctg
     9961 ttcgtccccg tcgaagccgc gtccgagacg ccgcttcgcg atcggcgcgt cggccgtgcg
    10021 tacctctgga cgcctagcca tcaggagagg cgcttgcttc aaaccgactg ggagcgtctc
    10081 gccgagatga tcgccgcggg ggacgtcgag cggatcaccg gtcacctcgg tgaggtcttg
    10141 caggttcggc cgaaggcccc gcatgggcgg agtcgtcgcc gtgcacccga tgaagaaggt
    10201 gcggtgcagt ggacgatgcc gcgcgggttc tacttgcggc cttcattcac cgggtcggtg
    10261 cttgcgcgac ttcgcgcgga ggagctcgat ggcgcgaggc gctgagctgg tggctggtga
    10321 ctcgcgcgcg gcggcacccg tctaaagggg cgatgccccg caacgcgtga accagggcaa
    10381 gacgtcgagg ggcgcatcga ccgtcacgtt cgcgcggccg ctgagctctt cgccctcccc
    10441 gtgcagctgc cagcaccctt cgggcaacca gacctcgcgt tgggtggcgc ccttcgcgag
    10501 aaccggagcg atgagcaggt ccgggccgag catccattgg tcgtcgttgt gcgggttgcc
    10561 ctcggcgctt gggtcgtgaa gccacatcgg gcgcgtgatc ggcatgcctg tgtccacggc
    10621 ttcctgccag agctgcaaga tgagaggccg ggcgcgcacg tgaagatcgg cggccgcttt
    10681 ccaaagcgct tcggccttgg gctcgggatc gccgggctcg aagaaccagg gcatgcgcac
    10741 tccgttgaag gccgagccgt gaactcggaa gtgggtcgtg aacaccgcgg cctgcgccca
    10801 acgcacgaag agctccttgc tggtgccggg gattccagtc cagtcagcgt accctccgat
    10861 gtcggtagag aacccatacg caccgccgac cgcccgattg agcatgtcgg gcacgattga
    10921 ggggagcccg ttctcctcgc tccaatcgac cgcttcgtct ccgggaaagg tcgcgctctc
    10981 gaacgccgcc gagcccgcac ggccgctgta cccggcacgg acgaagatga agatctcgcg
    11041 ttcgggatgt tcgacgaggt attcgtcgat cgcttcgcgc gtggcctcgt gctggaggcg
    11101 cgggtacgcg ttgtgcatgg tcgctccggt gctgccgtcg gagaagtgca tgtctgcgat
    11161 gacctgctct ccgaaatcgt tcatgaagcc gtcggcaccg aggtcgagca tctgctgaac
    11221 gaggcccttc caccaggaac gggcttcagg gttcgtgaag tcgatgaccg cggcctctcc
    11281 accgtcgaag gagttcggat agatgtacgc cgcacccgcg gcggtcgtgg cgacgtagcc
    11341 ctgatcgacc gcttcggcga agcgtcccga cggctccgtg agcaccaaat cctccgcaac
    11401 gaaggggcga atatagagga tcgcgcggat gccctcccgg gcgagccgct gaatcacctc
    11461 ggaaacgaaa tccggttcga gcctcgccca gccctcgaat gcataggcct cgacaccgag
    11521 gtcgcgggac tcgatcatcg ccaagtcgtc gaggactcgg tcccgatagg tttccgcggt
    11581 ttcgaccccg ggaaacgtga tcacggtgcg cgatagcgtc ggcccgaggc tccacgccgg
    11641 cgcgacgcga tgccgtcccg tcgcctcggt gagaagcgct gtcgctctag cgccgtcgcc
    11701 gggtgcgatc atcaaagaga ggcggtcgcc cgcagcgatg aggcgccatg cgtcgtcgag
    11761 gtcggacgcc agccgccagc tcgccagggc gtcggtgtcg atgaagaggc catagccctc
    11821 cgatgagatg aactggggtt gcgcgtagta gtagccggtc gtcagatcgg ggaaacgata
    11881 gtcttgaacc cagttctgga atgcaaggcc gcggaggtct gtgctctcgc ggcgaccgcc
    11941 gaatccgtga aacgcctcgt ccggaggcga agcaaagctg gcgaatactg ccgagacgcc
    12001 cgcggcatcg ctcacctcgg cgtcgaccgc caaggcgccc gtttcatcgg gcgcgatccg
    12061 gagggtcatg gtccgtccat ccgggtcgtc ggtatcgagg acgacgagca gcgcatcgcc
    12121 gctgcgctcg atgcttcgaa cctccgtggc cgagtggcgt ttcggcggga ccggattcgt
    12181 tgggtttgga tcgggctgcc cgccgaagcc cgcgaggact tgatagcgga gctcgggatc
    12241 gtctccggtg acgaagccca gcggcgcgta gagcgtgtcg ccggcgtcct ccgttcgagc
    12301 caaggtggta aggaccagct tcccggtgtt gtcttctacg cggagcgaga acggatcggt
    12361 catgacgcga acgcgtccgc tctcgctgct gaccgtgtct gtggctgagc catcgctgct
    12421 gcacgccgtg agcacgcacg ctagggtgaa taagacgagt ccagaacggg cgattccatt
    12481 aagcatattc atttgcattc gatagctcag ccagcccttg ggcggtagtc gatcgtgcgg
    12541 tcgtcggacg agagggggat ccgcgggacc gggctcatag cgcgggtacc ggggcagcta
    12601 tgcgtttccg ttgccgagct agtcttcgcg gactctcaag gtgacttgac cgacggtgcc
    12661 gccgacgagc ttggcctggc aacagaggcg caccttgtcg ggcgtgtcac cgaagatctc
    12721 gagcacctcg agctcgaact cgtcggccgc ggcgagggct tcgcccccct cgagcacgtc
    12781 gactctgcac gttccgcagg tggcggcccg gcaagaataa gggacagcgg cggcggggtg
    12841 cgcgtcggtg atgtccatga tcgtttcgcc tggttgtgcg tcgacctcga agccggaggg
    12901 ctcaaaaacg acggattcga tgcgcaggag ctcttcgatc gcgttgggaa tcgtggatgg
    12961 atcttggatc atttcgcgac gctgatcggg atagtcgtcc agccaccgca tcgagtttcc
    13021 gattggattc gtcgtggtgt cgttccagcc cgctatcatc acgaagcaga agccgagtat
    13081 gtcccaatcg ctgagcgtgt cgccatcgat ctcggcctgg gtgagcttgc tgatcatgtc
    13141 gtcggttggg ttcgcgcggc gggcctggca catcttgccg aagtacgtga ccagcccctg
    13201 cacggcgccc gcggcctgct tcagatcgat gtcgaaatcc gcggaagccg aggtgatgcc
    13261 atcggaccaa gggtcgaact gctcgcgatc ttcggcaggg acggcgagga tgttggccac
    13321 taccatcgtc ggaagcggag aggcgatctc ctcgatcagg tcgaccgcta ctccatcggc
    13381 ggccttctgc ttcatcgcat cgacgcgttc gacgacgaac tgccggatcg tcggctcgag
    13441 ctccttcacc gtgtcgggcg tgaacgcctt ggtgatcaga cggcggagcc gcgtgtgatc
    13501 gggcggatcc atcatgatga aggtcggggg aattccgagc ttgctgatct catccttggc
    13561 gggcgtcagg ccttgtgccg aggagaagat ctggggtctt cggagcgcgt ggaagatgtc
    13621 gtcgtagcga gagagcacga cgaagttcat tcgctcgttg tggtaaaccg gatgctcgtc
    13681 tctcagccgc tcgtagaacg gatagggatt ggcgatcacc tccgggttga agggatcgta
    13741 gatcgggtcg tagtcgattg ttggtgcctc gcctgccgcg cccatcgtct ccagtccttc
    13801 tgccgatcgg ccgcccacag ccacggcgtc aagactagga cactaattga gtttggccgg
    13861 tcgtcgcgag agatgcgcgc tccgaagaat gccccctcaa ccgccgctcc ctaccccgaa
    13921 cggtggcggt ggcggtggca cgctgctgcg gttgcgggcc acgccttctg ggatctcgta
    13981 gacgcggcag atgtcgtccc aaatcgtcgg gccgggctgg atcgcgttcc tgcccgtgta
    14041 cggtgaggcg gcgccgaggg cccaggacac gaacggcttt agcggcgcga gcgcaacccg
    14101 ttgcttccgg tgacgcttgc ggagctcctc catgaaggcg tcgtgaccga tgcgctgaaa
    14161 gcggagcagc tgctcgctct gtttcccgtc catccaacat tcgtaaaacc atccggcgtt
    14221 gctcctcggg tcgggcccgg gccgaaacgc acgctctggc ggcaccggta gaccgatggc
    14281 tccaaacatg tcttttcgaa gccggcccgc tggcagcttc caggaatcgt cgccgccgat
    14341 gagcaagacg tggccgtccg gctgcacgac cgccgctgct gcaatcgccc tcgccgcgtc
    14401 gcgcgctgcg atgccgtgct ccggctgatc gagcgacacc atgaagacga agggccgata
    14461 gctcggatgc ctgggcatca tgtcccgcag atccatcacc gcgccgagcc gcagcgagca
    14521 ctgccgcagt cctgaaaaac ggagccagct ctcggcggtg agcttctgca ggccatagtt
    14581 gtcgtctggc cagcaagggt cggtcgattg gcgattcgga tcccgcgggc cgcaagggcc
    14641 gaacgccgca tagctcgaca cgaacacgaa gaacgcatcg ggcgcatgct tccgcgtcgc
    14701 gtcgatgagg tgctgagtcc catgaaggtt caccgcttcg gcgcgctccg gttccgcata
    14761 ggcgatcggc gcaacgaccg ccgcgcagtg aacgaccacg ttgggcttca cgcgagcgac
    14821 gagccgcagc accgcattgg cgtcggtgag gtcgcagggc tcgaatcgag tctccg
//
LOCUS       DASRZF010000236         3924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2918689, whole genome shotgun sequence.
ACCESSION   DASRZF010000236 DASRZF010000000
VERSION     DASRZF010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3924
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2918689"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..111
                     /locus_tag="VFG22_10395"
     CDS             <1..111
                     /locus_tag="VFG22_10395"
                     /inference="COORDINATES: protein motif:HMM:NF019274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Kazal-type serine protease inhibitor
                     domain-containing protein"
                     /protein_id="HET6416692.1"
                     /translation="CTADYNPVCGCDGKTYANACSAKANGVSVKQGGACP"
     gene            350..1201
                     /locus_tag="VFG22_10400"
     CDS             350..1201
                     /locus_tag="VFG22_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HET6416693.1"
                     /translation="MRWKGRRQSAHVEDRRGARSGTKLAGGGLGLLLLVGVGLFLGVD
                     PSTLVQVVQIQQESSAGPVANGVPAEDEQARFVSVILADTEDTWKIIFRAAGRDYQEP
                     TLVLFSGAVRSACGFAQAAMGPFYCPADQKVYIDLSFYSDLQDRFHAPGDFAQAYVIA
                     HEVGHHVQTLLGISEEVQRKRAGLGEVESNRLSVRQELQADCLAGIWAHHADRTRGLL
                     EDGDIEEGLNAASAIGDDRLQKQSQGYITPESFTHGSSAERVHWFKRGYATGDVGDCD
                     TFEGARR"
     gene            complement(1246..2034)
                     /locus_tag="VFG22_10405"
     CDS             complement(1246..2034)
                     /locus_tag="VFG22_10405"
                     /inference="COORDINATES: protein motif:HMM:NF017551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HET6416694.1"
                     /translation="MCTIIVRHGMDDWCTTIIASNRDEFYDRPSSGPLVLRADPLVIG
                     GRDEREGGTWFGLTPAGFFAGLTNQRSFGVRHDTLRSRGGLVVDVLAKRSLEEAKRFL
                     AGVDPRSYNEFNLIFGDGVELCVAYGRGEEAEIEIEPISSGVHVLCNDRLGSPEFPKA
                     DKARERVASIPPAPWHGLRGGLAEVLADRSLPDPAEVPSLPPDAPFDREVARRLQAIY
                     VETPVYGTVSSTIAAVSPGRVHEYQFTEGLPTEVGFKDMLNLTR"
     gene            complement(2038..2892)
                     /locus_tag="VFG22_10410"
     CDS             complement(2038..2892)
                     /locus_tag="VFG22_10410"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET6416695.1"
                     /translation="MRADSPSGTSLLVSFARGLGVEDRPLDSLAAELLPPWLGRIASA
                     PRYLGVAGSFYRNALRASSFGLVDHAVLRTQAIDTHLVSALRSGIEQLVILGAGLDAR
                     AWRLPALQAATVFEVDHPATQRYKRKRIGSRVAPTDVRYVPVDFEKEQFSPSLVRAGF
                     RMEDPSVWIWEGVTMYLHRQAIGQTLEQLTKLSASGSELAVTYLVPNSLPFGALGRFA
                     IPAMFKAAGEPLKGALDSRELATAIAPSWEVVYDQDSRGWQELTRSRARSPQTFRSER
                     LAVAKRRA"
     gene            complement(2896..3705)
                     /locus_tag="VFG22_10415"
     CDS             complement(2896..3705)
                     /locus_tag="VFG22_10415"
                     /inference="COORDINATES: protein motif:HMM:NF013782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HET6416696.1"
                     /translation="MRVINTVWEFPLTLPRNAFTAREAPRAGDLWRLCQDVATLASIR
                     SGWPPSRFRAEKVAFIVYKMTMRHQAETPYGAVLDTKTWVSRLRRRTLSTREVRVRIA
                     DQPVASATQEWVHVDFETQKPKQGSEETAAAFPEVDIEPSVTMPGYDPLPGAEQELVF
                     DMWQTWSDPLAHANHPQYIDWCDESTSRRMLASGLDPVLLQPLAEQVTFRSSVLPGER
                     VSVRTERVGVIGGNAVVLKHHLATERGPAADAVSVRSLAEGSAEALISAWD"
     gene            complement(3743..>3924)
                     /locus_tag="VFG22_10420"
     CDS             complement(3743..>3924)
                     /locus_tag="VFG22_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416697.1"
                     /translation="RYAKILDDVPPERVICYCGSGVTACHLLLAMEACGLIGAQLYVG
                     SWSEWCRQARPGAVG"
BASE COUNT          746 a         1233 c         1276 g          669 t
ORIGIN      
        1 tgtaccgccg actacaaccc cgtctgcggg tgcgacggga agacgtacgc caacgcctgt
       61 tcggccaaag ccaacggcgt gtcggtcaag caaggcggag cttgcccgta gcgcctcacg
      121 cccgggcacc gagccaccga accgttgccg atctccaccg gtcagttgtt gacgtcggca
      181 cgggggggct tctcgcgcgc atcatgtcca gccatgagtg ctacaggaga aggtcaatgt
      241 aggtacagtc agggtccagc ccctacgacc gaaaatcgtt tgctccaaga agcatgctgt
      301 ccgtatgctc acggctggtt tccctagcga ccctctggaa aggctcactg tgcgctggaa
      361 aggccgaaga caaagtgcgc acgtcgagga tcggcgcggc gcacgttccg gaaccaagct
      421 cgcgggcggc ggactgggct tgctgctgct ggttggggtg ggtctgttcc tgggggtcga
      481 cccgagcacc ctcgttcaag tggttcagat ccagcaggaa agcagcgctg ggccggtcgc
      541 caatggggtt cccgcggagg acgagcaggc gcggttcgtt tcggtaatcc tcgcggatac
      601 ggaagacacc tggaagatca tctttcgcgc cgcagggcgc gactaccagg aaccgacgct
      661 cgttctgttc agcggcgccg ttcgttcggc ttgcggcttc gcccaggccg cgatgggacc
      721 gttctattgc ccggcagatc agaaggttta catcgacctc agcttctact cagacctcca
      781 agaccgcttt cacgcgccgg gggatttcgc tcaggcgtac gtcatcgcgc atgaggtcgg
      841 tcaccacgtg cagaccctgc ttggcatctc cgaagaggtg caacgaaaaa gagctgggct
      901 aggcgaagtc gagtcgaacc ggctttcggt gcgccaggag ctccaagcgg attgtctagc
      961 gggcatttgg gcgcatcacg cggaccgaac ccgtggtttg ctcgaagacg gcgacatcga
     1021 agaaggcctc aatgcggcga gcgccattgg cgacgaccgc ctgcagaagc agtcgcaggg
     1081 ctacatcacg ccggaatcgt tcacccacgg gagctcggcc gagcgcgtgc actggttcaa
     1141 acgcgggtac gcaactggag atgtcggcga ctgcgatacg ttcgaggggg ctcggcggta
     1201 acgagcagag tgtagccggc gctcacactg aggcccgcct gcggattaac gggtgaggtt
     1261 gagcatgtct ttgaagccaa cctccgtcgg gagcccctcg gtgaactggt actcgtggac
     1321 gcgtccaggg ctgaccgctg cgatggtcga cgagacggtg ccatacaccg gcgtctcgac
     1381 gtagatcgct tggaggcgcc gcgcgacctc gcgatcgaag ggtgcatcgg gagggagaga
     1441 cggcacctcg gcaggatcgg gaagcgagcg atccgcgagc acttccgcca ggcccccccg
     1501 gagcccgtgc cagggtgcgg gtggaatcga ggcgactcgc tcccgcgctt tgtccgcctt
     1561 ggggaactca ggagagccga gcctatcgtt gcagagcaca tggacgccgc tcgaaattgg
     1621 ctcgatctcg atctcggcct cctcccccct tccgtaggcc acgcaaagct cgactccgtc
     1681 gccgaagatc agattgaact cgttgtagct ccgaggatcg accccagcga gaaagcgctt
     1741 cgcctcttcg agagaacgct tcgcgagcac atccaccacg agcccgccgc gagagcgcaa
     1801 ggtgtcgtgc cgcactccga agctcctctg gttggtcagc ccagcgaaga aaccggcagg
     1861 agtcagaccg aaccaggttc ccccttcgcg ctcgtcgcgc ccaccgatca cgagcgggtc
     1921 cgcacgcaga acgagcggcc ccgatgacgg ccgatcatag aattcgtccc gattcgacgc
     1981 gatgatcgtc gtacaccagt cgtccattcc gtgccgaacg atgatggtgc acatcgctca
     2041 ggccctccgt ttcgcgaccg ccaagcgctc ggagcggaag gtctgcggcg aacgggcgcg
     2101 cgagcgggtg agctcctgcc agccgcgtga gtcctggtca tacacgactt cccagctcgg
     2161 cgcgatcgcg gtggcgagct ctcgcgagtc cagcgcacct ttgaggggtt cgcccgcggc
     2221 cttgaacatc gcgggaatcg caaaacggcc gagggccccg aacgggagcg aattcggaac
     2281 gagataggtg accgcaagct cgctgcctga agcggatagc ttggttagct gctcgagggt
     2341 ctgcccgatc gcctgccggt gcagatacat cgtaacgcct tcccagatcc aaacgctcgg
     2401 gtcttccatc cgaaacccgg cgcgtacgag gcttggcgag aattgctcct tttcgaaatc
     2461 gaccggaacg tagcgcacgt cggtgggagc gaccctgctg ccaatgcgtt tgcgcttgta
     2521 gcgctgcgtg gcgggatgat cgacttcaaa cacggtcgca gcttggaggg caggcaatct
     2581 ccaggcgcgt gcgtcgagcc ccgcacccag gatcacgagc tgctcgatgc cagaacgcaa
     2641 agccgaaacg aggtgcgtgt cgatcgcttg ggtacgcagc actgcgtggt cgaccaatcc
     2701 gaagctcgag gctcgtagcg cgtttcggta gaatgaaccg gccacgccga ggtaccgagg
     2761 cgcgctcgcg atgcgcccga gccaaggtgg caagagctcc gccgccaacg agtcgagcgg
     2821 ccgatcttcg actccgaggc cccgcgcgaa ggaaacaaga agagacgtgc cgcttggaga
     2881 gtccgcgcgc accggtcaat cccaggccga gatgagggct tcggcgcttc cctcggcaag
     2941 agaacgcacg ctcacggcgt ccgcggcggg cccgcgttcg gtcgccaaat ggtgtttcaa
     3001 gacaaccgca ttgccgccga tcacgccaac ccgctccgtt cgcacgctta ctcgttcgcc
     3061 aggaaggacg gagcttcgaa aggttacttg ctcggcgagc ggctgaagca ggacggggtc
     3121 caggccggac gcgagcatgc ggcggctcgt cgactcgtca caccagtcga tgtactgggg
     3181 gtgattggcg tgcgcgagcg gatcggacca ggtctgccac atgtcgaaca cgagctcttg
     3241 ctccgctccg ggcagtgggt cgtacccagg catcgtgacc gagggctcga tgtcgacctc
     3301 cggaaaagca gcggcggtct cctccgagcc ctgctttggc ttctgtgtct cgaaatcgac
     3361 atgaacccat tcctgggtgg cggacgcgac cggctggtcc gcgattcgca cccgcacctc
     3421 acgagtactg agcgttcggc ggcgaaggcg cgatacccag gtcttggtgt cgagcacggc
     3481 gccgtagggt gtttcagcct gatggcgcat cgtcatcttg tacacgatga acgcaacctt
     3541 ttccgcgcgg aacctggacg ggggccatcc cgagcgaatc gaggcgagcg ttgcgacatc
     3601 ttggcagaga cgccagaggt ctcctgctct tggagcctcg cgcgctgtga acgcgtttcg
     3661 aggcaaggtg agcgggaact cccaaaccgt gttgatcacc cgcatgagat agcatctcga
     3721 gcgctgggtg cgaccggccg gatcagccga ctgcgccagg acgcgcttgg cggcaccatt
     3781 cgctccagga gccaacgtac agctgcgcgc cgattaggcc acaggcttcc attgcaagga
     3841 gaagatggca ggccgtcacc ccggacccgc aataacagat cacacgctct ggggggacgt
     3901 cgtcgaggat cttcgcgtaa cgtt
//
LOCUS       DASRZF010000237        14287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2232204, whole genome shotgun sequence.
ACCESSION   DASRZF010000237 DASRZF010000000
VERSION     DASRZF010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 14287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14287
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2232204"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..1453)
                     /locus_tag="VFG22_10425"
     CDS             complement(86..1453)
                     /locus_tag="VFG22_10425"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HET6416698.1"
                     /translation="MNPTIHGGLGLLLLASFALAPTARAQSAKGLLDKMLEEESKRAK
                     GVDDYAMDISMMGHDTTLFYERVSAKAPNGKPIDTFRLVPFEEMKSRQESGQGMPPEA
                     WQAYSDALRQTGSALSSETEKGMNEAGLPAGMLGAMDSGASAEPWASPNPSTMMNSMA
                     DFAEAAGSAPTADDAVADEAATAKSMALFRKRAKIVGKESIGKRRAIQARADDLNLVE
                     EANGEELTIQTVSLWVDAEKHVPLKMRMEGVAKRDGKSREIFIERLDQDYRTVPGSKL
                     YMPYMSLVRMHGILGDDQQRQMEDARRQLAEFDQQLAEMPPEQRAQVERMAGSQIAML
                     RKMVDSGEMDMVTKVRAIRVNTGLGKAVPAAAQSRPTPSTMPSSTGDPLVQSIQRDLV
                     ALGYDPGNTRGELSTPTVVAISKFQAQNNLEVTGEATPQLAGILAAKRDAAASSAATA
                     SSGTE"
     gene            complement(1562..2773)
                     /locus_tag="VFG22_10430"
     CDS             complement(1562..2773)
                     /locus_tag="VFG22_10430"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET6416699.1"
                     /translation="MNTKIKTSESLRGVRYEIRGQLAQRAHEMERQGYEIISMNIGNP
                     GLFGFRTPETMRMAMIENLPSSEAYCHQKGIFPAREAVVMQQQERGVLNVTAEHVFMG
                     NGVSELIDLTLRALLDDGDEVLIPSPDYPLWTAAVRLNRGRPVHYPCPPEKGFQPDLD
                     ALKGLISKRTRAIVIINPNNPTGAVYSREVLEAMVALAEEHKLVIFSDEIYDGMVYDG
                     AEFIPVAPLVKNTLCGTFSGLSKVYRACGFRVGWVVFNGSNELAKDYLQGLDLLASLR
                     LCSNVPGQWGVQTALGGYQSLKDLIRPSGRLYESRQAVIRGVEASEYLTLVTPRGSMY
                     AFIGADTNKLPNFDDQEFAMELLETEHVLVAPGSSFNVDYRNYFRVTILPQAKVIDDV
                     FRRIEKVLRDK"
     gene            complement(2838..3197)
                     /locus_tag="VFG22_10435"
     CDS             complement(2838..3197)
                     /locus_tag="VFG22_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416700.1"
                     /translation="MGQRVLDVGRTAVDDYVRVYAHCSYERPGAVTLLAINLDPDQSV
                     RLEVEGIPSTGKELYLLSADALDSSDIALNGVILRDADGALPALEPRQIGRNPADLPP
                     RTMAFVVFPDAEAAACQ"
     gene            complement(3197..3712)
                     /locus_tag="VFG22_10440"
     CDS             complement(3197..3712)
                     /locus_tag="VFG22_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416701.1"
                     /translation="MLTAAQVDGIFEFAEALDYDVMFTIGAGKGVRDEDKAWTPDMAR
                     ALLGEFYHELSFGGVSSAYWPSLDEVTKVYPDFLQMGGANLIDVITWHYYPQQSERGG
                     SVVSDPWERGLLLDPERLDIVFDWADEIVDYRDADAPGTPIWFGETGHAQYGGQRIRD
                     RTIGHPSYGVG"
     gene            complement(3737..4099)
                     /locus_tag="VFG22_10445"
     CDS             complement(3737..4099)
                     /locus_tag="VFG22_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416702.1"
                     /translation="MRNLTYAIFAAGLLGACNDGGMGTLAPADTTAELSIDASRVVAT
                     VDERYLSVAVDTAGVVGGRFWSEDGSVELVGQERIPEYDFARPRLRALAAGLAPAFLR
                     IGGTDADRVIYDMSADPI"
     gene            4242..5063
                     /locus_tag="VFG22_10450"
     CDS             4242..5063
                     /locus_tag="VFG22_10450"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6416703.1"
                     /translation="MNRDAQRPSSGDPRLPSGQAEWPADAGRDRLIGDWHIYQRAGGH
                     RTSTDDLITAWYAVHRNAGSPRLYLDLGCGVGSVLLMVSHKLRPKIAVGVEAQDQSVV
                     MAARAIAELPTHSTRIELRHADFRDLDFAGERFDLITGSPPYFPMNTGVLPEDPQRRA
                     CRFEERGGVEAYVETAARLMGEHARFYLVFQTLWSERVSSSAARHDLHLSGRADFHMR
                     ADKSEPFLSVFELSKRPVDSPHSFRCSVRDADGAVSPEYQRIRLELGVGGFNDSR"
     gene            5181..5909
                     /locus_tag="VFG22_10455"
     CDS             5181..5909
                     /locus_tag="VFG22_10455"
                     /inference="COORDINATES: protein motif:HMM:NF024919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HET6416704.1"
                     /translation="MKKPHTAQLQLDILPQPGDTTCGPTCLHAVYRYWRDEVPLDALV
                     TEVKPLPEGGTLAVSLATHALQRGYDAEIYTYNLQLFDPSWFAGGVDIAERLEAQRKH
                     KRTQKLRISTDAYIEYLRLGGIVRFEELRPALIRRFLNRGVPILTGLSATYLYGCARE
                     HEDEYDDLRGEPVGHFVVLSGYDRKKRQVTVADPSHDNPRFRSHRYSVPMTRLITAIA
                     LGVMTYDANLLVLTPKPAVGETAA"
     gene            5906..7357
                     /locus_tag="VFG22_10460"
     CDS             5906..7357
                     /locus_tag="VFG22_10460"
                     /inference="COORDINATES: protein motif:HMM:NF020032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family protein"
                     /protein_id="HET6416705.1"
                     /translation="MTTLIVVERTANWPLEIPGTEVVAASDYLTNPRFVEMKRAKVFN
                     ICRSYGYQSAGYYVSLLAAARGHKPLPSVMTMQDLRQSSMVRIASETTELLLQQTLAP
                     IKADRFVLSIYFGRNMAKRYDRLCRALFNHFPAPLLRAEFVRGDQWRLEKLRVIASHE
                     IPESHHDFVVEQAKRFFDRPRPSGVKRARYDLAILVNPDEVDAPSDPKAIRRFIRAAK
                     KVGLDAWVVGKEDFGRIAEFDALFLRETTAVDHYTYRFARRAEAEGLVVIDDPESILR
                     CTNKVYQAELFEQNGVDCPKTVVVDRDSAHLVAERLGLPCVLKKPDSSFSAGVVKANN
                     ASELAAHLKQFLQESELVIAQEFVASEFDWRIGVIDRKPLYACRYHMARGHWQIQKAT
                     GESERLYGKTDTIAIEDAPKAIVDLAVRASGLIGDGLYGVDIKEVDGRLLVMEVNDNP
                     SIDSGTEDEILKDDLYLTIMQSMYDRLERRGRQ"
     gene            7354..8598
                     /locus_tag="VFG22_10465"
     CDS             7354..8598
                     /locus_tag="VFG22_10465"
                     /inference="COORDINATES: protein motif:HMM:NF016032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HET6416706.1"
                     /translation="MTASTTLHLFDAFGIEIEYMIVDAKTLSVRPIADELLKTVAGDY
                     EMEVDRGALRWSNELALHLIELKTNGPVASLANVHESFQRDIGAIDDLLRPMGARLLP
                     SAMHPWMGTDELRLWPHEDDAIYAALDRVFDCRGHGWSNLQSMHINLPFADDTELERL
                     HAAIRLMLPILPSLAASSPFVDGAASGYLDTRLEVYRHNAKRIPSVSGRVSPEAVFSK
                     SDYEALLESIYRDLAPLDPDGTLQHEWINARGCIARFDRMALEIRLIDVQECPRMDLA
                     IAGAALAVTQALVEETWAPLADQRAWHETELEKILLSTIADADHATLDAPRFLEAFGL
                     TGTRSIRAGELWQHLIETQVETRPEYAAAGDALRIISREGCLARRIMRASGPNPSPES
                     LREVYLQLADCLARGQAFSSAP"
     gene            8595..9302
                     /locus_tag="VFG22_10470"
     CDS             8595..9302
                     /locus_tag="VFG22_10470"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HET6416707.1"
                     /translation="MKGYALVLSCEHGGASVPQRYRHLFDSKPARDALRGHRGSDLGA
                     LRLARSLERRHRVPLYASTVTRLLVDLNRSLGHPQLFSEFTRDLDPAERESLLDRHYF
                     PHRTAVESWIDARVRGGDQVLHLGVHSFVPQIDGRARTADIGLLYDPSRSPERRFCDR
                     WKSSLHEIAPELRVRRNYPYLGKSDGFTTHLRRALGSPQYLGIELEVNQRALRTDENQ
                     RFLARVISDSLRAVLRP"
     gene            9488..11710
                     /locus_tag="VFG22_10475"
     CDS             9488..11710
                     /locus_tag="VFG22_10475"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: protein motif:HMM:TIGR00178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HET6416708.1"
                     /translation="MTQKPTIQYTKTDEAPLLATYSLLPILRAFTAPAGITVELPDIS
                     LAGRILASFPEALKKEQRIPDALAELGDLAKTPEANIIKLPNISASVPQMKAAVAELQ
                     AKGYAVPDYPEEPKTEEERAIAERYGKVKGSAVNPVLREGNSDRRAPKAVKEYARANP
                     HSMGAWARDSKTHVSTMSTGDFFHNEKSITLPAATTVRIEHVAKDGSTTVLKDGLALL
                     QGEILDGTFMSKAALLTFLREQVCDAVGLGVLFSLHMKATMMKVSDPIIFGHAVRVYF
                     AKVFEKHGETLAALGANPNDGFGEVLARIESLPADQRAEIEADIAAAYEKGPALAMVN
                     SDKGITNLHVPSDVIVDASMPAMIRTSGQMWNAAGDTQDTKAVIPDSSYAGVYREVID
                     FCKEHGAFDPTTMGSVPNVGLMAQKAEEYGSHDKTFEIPSAGRVRVVDAKGNVLMEHE
                     VEAGDIWRACQTKDAAVRDWVGLAVRRARATGAPAVFWLDAKRAHDAQLIAKVKAYLP
                     EHDTAGLDIRIMEPAAATRFSLERISEGKDAISVTGNVLRDYLTDLFPILEVGTSAKM
                     LSIVPLMNGGGLFETGAGGSAPKHVQQFEKENHLRWDSLGEFLALAASLDFLSETHDD
                     PKVKVLATTLDAAATKYLLENKSPSRKVNQIDNRGSHFYLALYWAQALAKCDDPELRA
                     YFEPIAKALADNENKISEELLAVQGKPVDIGGYYLPDDALASQAMRPSPTLNAIIDAL
                     "
     gene            complement(11739..13175)
                     /locus_tag="VFG22_10480"
     CDS             complement(11739..13175)
                     /locus_tag="VFG22_10480"
                     /EC_number="1.2.1.68"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050269 - coniferyl-aldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006081 - cellular aldehyde metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl aldehyde dehydrogenase"
                     /protein_id="HET6416709.1"
                     /translation="MAQTAQLRRIEIVENEAQRIFKLQRDAYLRDPYPSREVRAERLT
                     ALERILLDNSDAIVAAIREDFGHRCAEESKILEIFPCVDGIRHARKKLRKWMRPQRRE
                     VSLLFATGSNRVIPQPKGVVGIVSPWNYPLFLTVSPLISVLAAGNRAMIKMASNSQTL
                     CRLLQEKASEVFPEDTLAFLPGVPGRDFSSLPYDHIIFTGSADAGRTVMRSAAESLTP
                     VTLELGGKSPTIVCDDFDIDEAASRILYAKFINAGQTCLAPDYLFLPEAGRDRFVESA
                     KRIVAERYPDTNDGSYTSVIDDKSYRRLRMNLDDAEAKGAKLVPLVPGASFNDALRKI
                     PPHLVLDVTDDMMIMQEEIFGPLFPVMTYRDLDESIDYVTKRDRPLGFYVFTNDRSRQ
                     EKLLYSTISGGVTINNCIMHIAQHDLPFGGVGASGMGQYHGHEGFVEFSKIRPVFTNP
                     RLSLLHLFYPPYTGKQNRLIDFLLKWAR"
     gene            complement(13226..14149)
                     /locus_tag="VFG22_10485"
     CDS             complement(13226..14149)
                     /locus_tag="VFG22_10485"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HET6416710.1"
                     /translation="MEKEVLVVGANGRIGRWSVEAFLDAGWKVRAFVRTGSSARVRDG
                     VTVFEGDAFDAEAVTAASEGVAVIVNALNPPYERWADEVPKITRSILSAAEGSGATVM
                     LPGNVYNYGEGMPPVLREDTPHLPTTRKGRLREEIEEAYRQASARGIQTIVLRAGDFI
                     ERKATGNWFDTYVANKVDKGIVTYPGPLDRVHAWAYLPDLARAMVGLAAVRESLGAFE
                     TIGFGGFSLTGAELVETMERALERPLKVKRFPWGLVRLMGAFSAPMREVVEMRHLWEV
                     PHGIDGSKLARILPDFQPTACVTAIHEALER"
BASE COUNT         2692 a         4520 c         4371 g         2704 t
ORIGIN      
        1 ctttcatgaa tctgccaaac ccctgcttct tctttttgac cgcatcgatc ttctgctgga
       61 ggcacgccgc ctgggcttcc tccaattatt ccgtgccgga gctcgcggtc gccgcagagg
      121 acgcagcagc atcgcgcttc gccgcgagaa tgccggcgag ctgcggggtc gcctcgcccg
      181 tgacctcgag gttgttctgc gcctggaact tcgagatggc aacgacggtc ggcgtgctca
      241 gctcgccacg cgtattgcca gggtcatagc cgagggccac gagatcgcgc tggatcgact
      301 gcaccagcgg atcaccggtt gaagacggca tagtggatgg ggtcggccgg gattgcgcgg
      361 cagccgggac cgccttgcca agccccgtgt tgactcgaat cgcccgaacc ttcgtcacca
      421 tgtccatctc gccgctgtcg accatcttgc gtaacatcgc gatctgcgag cccgccatcc
      481 gctctacctg ggcgcgctgc tcgggcggca tctcggcgag ttgctggtcg aattcggcaa
      541 gctgcctgcg tgcgtcctcc atctgtctct gttggtcgtc gccgaggatt ccgtgcatgc
      601 gcaccaggct catgtagggc atgtagagct tgctgcccgg caccgtgcgg tagtcctggt
      661 cgagccgctc gatgaagatc tcgcgtgact tcccatcccg cttcgccacg ccctccatgc
      721 gcatcttcaa cgggacgtgc ttctccgcgt cgacccaaag gctgacggtc tggatcgtga
      781 gctcctcacc gttcgcctcc tcgactagat tcaggtcatc tgcacgggcc tggatggcgc
      841 gccttttccc gatcgactcc ttgccgacga tcttcgcgcg cttcctgaag agggccatgc
      901 tcttggccgt tgccgcctcg tccgcgacgg cgtcgtcggc cgtgggtgca ctgccggctg
      961 cctcggcgaa gtcggccatg ctgttcatca tcgtgctcgg attgggcgac gcccagggct
     1021 ctgcgctcgc gccactatcc atggcgccga gcattccggc tgggaggccg gcttcgttca
     1081 tgcccttctc ggtttcgctg ctcagtgccg agccggtttg tctgagtgcg tcggaatagg
     1141 cttgccaggc ttctggcggc atgccttgcc ccgactcttg gcggctcttc atttcctcga
     1201 acggcaccag gcggaacgtg tcgatgggct tcccgttcgg ggctttggcg gacacgcgct
     1261 cgtagaagag ggtcgtgtcg tgccccatca tgctgatgtc catcgcatag tcgtcgacac
     1321 ctttcgcgcg cttgctctcc tcctccaaca tcttgtcgag gagacccttg gcgctttggg
     1381 ctctggcggt cggggcgagg gcgaacgagg cgagtagcaa gaggccaagt ccaccatgga
     1441 tggtcgggtt catgagcacg gtcctctcac cagaagtata ccgcatgggc gcgcactcca
     1501 cgattgaaca acacgaccgg ttcgtcgata ccttcacccg atccgagccg cgatgacgtc
     1561 attacttgtc ccgtagtacc ttttcgatcc ttcggaacac gtcgtcgatg accttcgctt
     1621 gcggcaagat ggtgactcgg aagtagttgc ggtagtcgac gttgaagctc gagcctggcg
     1681 ccacgagaac gtgctctgtc tccagcaact ccatcgcgaa ttcttgatca tcgaaattgg
     1741 gcagcttgtt cgtgtcggcg ccgatgaagg cgtacatgga gccacggggc gtgacgagcg
     1801 tcagatactc gctggcttcg accccgcgga tcacggcctg gcgagactcg tacaggcgtc
     1861 cgctcggacg aatcagatcc ttcaggctct ggtagcctcc gagcgccgtc tgcacgcccc
     1921 actgcccggg tacattgctg caaaggcgca gagacgccag caagtcgagg ccctggaggt
     1981 agtccttcgc gagctcgttg ctgccgttga acaccaccca gcccactcga aaaccgcagg
     2041 cgcggtagac cttggacagg ccgctgaacg ttccgcagag ggtgttcttc acgagcggcg
     2101 cgaccgggat gaactcggcg ccgtcataga ccattccgtc gtagatctcg tcgctgaaga
     2161 tcaccagctt gtgctcttcg gcgagcgcga ccatcgcctc cagcacctcg cggctgtaca
     2221 cggcgccggt gggattgttc ggattgatga tgacgatcgc ccgggtgcgc ttgctgatca
     2281 gacccttcag cgcgtcgagg tccggctgaa accccttctc cgggggacag gggtagtgca
     2341 caggccggcc gcgattgagc cggaccgccg ccgtccagag cggatagtcg gggctcggga
     2401 tcagcacctc gtcgccgtca tcgagcagcg cgcgcaaggt cagatcgatc agctcgctga
     2461 caccgttgcc catgaacacg tgctccgccg tcacgttcag gacgccgcgt tcttgttgct
     2521 gcatcacgac ggcttcccgc gcagggaaga tgcctttctg atgacagtac gcctcgctgc
     2581 tgggcaggtt ctcgatcatc gccatgcgca tcgtttccgg cgtgcgaaaa ccaaagaggc
     2641 cagggttgcc gatgttcatc gagatgatct cgtagccctg gcgctccatc tcatgcgcgc
     2701 gctgcgcgag ctgtccacgt atttcgtagc gaacgccgcg caggctctcg ctggtcttga
     2761 tcttggtgtt cattggctcc gctcggatga tagccgtctt gggacggatt ggcttccgac
     2821 caggcgcgga tctcggctca ctggcaggcg gcggcttcgg cgtctggaaa gacgacgaag
     2881 gccatcgtgc gcgggggaag gtccgcaggg ttccgcccga tttgcctggg ttcgagagcc
     2941 ggaagggcgc cgtcggcatc gcgcaagatg acgccgttca acgcgatgtc ggacgaatcg
     3001 agcgcgtccg cgctcagcag gtagagctcc ttgcccgtgc ttggaatccc ctcgacctcg
     3061 aggcgaacgc tctggtctgg gtcgagattg atggcgagca aggtcacggc gcccgggcgc
     3121 tcgtaggagc aatgcgcata gactcgtacg tagtcgtcga ctgcggttct ccccacatcg
     3181 aggacgcgtt gccccatcaa ccgacgccat aggacggatg cccaatagtc cggtctcgga
     3241 tccgttgtcc tccatactgg gcgtgccctg tctccccgaa ccaaatcgga gtaccggggg
     3301 cgtccgcgtc ccgatagtcc acgatctcgt cggcccagtc gaacacgatg tccaggcgtt
     3361 ccggatcgag gagcagccca cgctcccagg ggtcggagac caccgaaccg ccgcgctccg
     3421 attgctgcgg gtagtaatgc caggtgatca cgtcgatcag gttggcgccg cccatctgga
     3481 ggaagtcggg atagaccttg gtgacctcgt ccaggctcgg ccagtacgcg ctagaaacgc
     3541 cgccgaacga gagctcgtgg tagaactcgc cgaggagcgc gcgcgccata tccggtgtcc
     3601 aggccttgtc ctcgtctcgc acccctttgc ccgccccgat cgtgaacatc acgtcgtagt
     3661 cgagagcctc ggcaaactcg aagatgccat cgacctgtgc tgcggtgagc acgaattcga
     3721 acccctctgg cgcttcctaa atggggtctg cgctcatgtc gtagatgaca cgatcggcat
     3781 cggttccacc gattctcagg aacgcaggag ccagtccggc ggcgagcgcg cggagccgag
     3841 gccgagcgaa atcgtattcc gggatgcgtt cctgtccgac gagctcgacg ctcccgtcct
     3901 cggaccagaa acgacccccg acgaccccgg ccgtgtcgac cgcgaccgag agataccgct
     3961 cgtccaccgt ggccacgacg cgcgaagcat cgatgctgag ctcggcagtc gtgtcagctg
     4021 gcgctagggt ccccatccca ccgtcgttgc aagctcccag gaggccagcg gcgaagattg
     4081 cataagtgag atttcgcatg ttgccctcat ttacggcaca caggccggag gcgccaagcg
     4141 aggcgagatg gcccaggatc cgtcgaacct cggcgcggtc gagctggtat ccgattgtgt
     4201 ttgaaggcgg aagagggcag gtctaggcct gggtcttcca aatgaaccga gacgcgcaga
     4261 gaccgagtag cggagatcca cggctgccat ccggacaagc ggaatggccc gcggatgcgg
     4321 ggcgcgaccg tctcattggc gattggcata tctaccagcg agccggggga caccggacca
     4381 gcaccgacga cctgatcacc gcttggtatg ccgtccatcg caacgctggg tcacccaggc
     4441 tctatctcga ccttggatgt ggcgtcggct ccgtgctcct gatggtgagc cacaagctcc
     4501 gtcccaagat cgccgtcggc gtcgaggctc aggatcaatc ggttgtcatg gcggcacgag
     4561 ccattgcgga attgccaacg cacagcactc ggatcgagct acggcacgcc gacttccggg
     4621 acctcgactt cgcgggtgag cgcttcgacc tcatcacagg gtccccgccc tattttccca
     4681 tgaacaccgg tgtgcttccc gaggatccgc aaagacgcgc ctgtcgtttc gaggagcgcg
     4741 gcggagtcga agcctatgtg gagacggcgg cccgcctcat gggcgagcac gcacggtttt
     4801 acttggtctt tcaaactcta tggagcgagc gggtcagctc ctcggctgcc cgccacgatc
     4861 tgcacctcag cggccgggcg gatttccaca tgcgcgcaga caaatccgag ccattcctca
     4921 gcgtcttcga gctctccaag cgacccgtcg attctccgca ttcgttcagg tgttcggtac
     4981 gggatgccga cggggcagtg agccctgagt accagcggat caggctggag ctaggtgttg
     5041 gcgggtttaa cgattcgagg tgattgcagc aggcaaggtg ctaatacgca cgtcccgagc
     5101 tcgatccgcg tgaacccccg gtgagcgacg aacccaggtc gggcccagtt gccgcgttca
     5161 tctctcgttc gcgttcagat atgaaaaaac cacacaccgc ccagctgcag ctggacatac
     5221 ttccgcagcc cggagacacg acctgcgggc cgacctgcct tcatgcggtg taccgctact
     5281 ggcgtgacga ggttccactc gatgcgctgg tgaccgaggt caagcctttg cccgagggcg
     5341 gaacccttgc cgtctcactc gcaacccatg cgcttcagcg cggttacgac gccgagatct
     5401 acacctacaa cctgcagttg ttcgacccga gttggtttgc aggtggcgtc gacatcgcgg
     5461 aaaggctcga ggcccagcga aagcacaagc gcacgcagaa gctcagaatc tccaccgacg
     5521 cttacatcga gtacctccgc ctcggaggca tcgtccgatt cgaagagctt cgtccggcct
     5581 tgatcagacg cttcttgaat cgaggcgtcc ccattctcac cgggctcagc gcgacgtatc
     5641 tctatggctg cgcaagagaa cacgaggacg agtatgacga cctgcgagga gagccggtgg
     5701 gccacttcgt cgtcttgagt ggctacgatc gaaagaagcg ccaggtcacc gtcgccgacc
     5761 cctcgcacga caatccacgc tttcgaagcc atcgatacag cgttccgatg actcgcttga
     5821 ttaccgcaat cgcactcggc gtgatgacct acgatgcaaa tcttctcgtg ctcacgccca
     5881 agccagcagt tggagaaacc gccgcatgac cacgctcatc gtcgtcgaac ggacagcaaa
     5941 ttggccgctc gaaatcccgg gcactgaagt cgtcgccgcc tcggactatc taaccaatcc
     6001 gagattcgtc gagatgaaga gggccaaggt tttcaacatc tgcaggagct atggctatca
     6061 gagcgccggc tactacgtct ctctcttggc ggcagcgcgc ggacacaaac cattgccctc
     6121 cgtgatgacc atgcaggacc tgcgccagtc gtcgatggtt cgcatcgcct cggagacgac
     6181 cgagcttctg cttcagcaga cgctcgcgcc gatcaaggcc gatcgcttcg tgctgagcat
     6241 ctacttcggc cggaacatgg ccaagcggta cgatcgactc tgtcgcgcgc tcttcaatca
     6301 tttccccgca ccattgctcc gcgcggagtt cgttcggggc gaccagtgga ggctcgagaa
     6361 gctccgcgtc atcgcgtcgc atgagatccc cgaatcccac cacgatttcg tcgtcgaaca
     6421 agcgaaacgc ttcttcgatc gacctcgccc ttccggagtg aagcgagcgc gctacgatct
     6481 cgcgatcctc gtcaacccgg acgaggtgga cgccccatcc gacccgaagg cgattcggcg
     6541 cttcatccgc gctgccaaga aagttgggct cgatgcctgg gtcgtgggca aagaggactt
     6601 cggacgaatc gcggagttcg acgctctgtt cctccgcgaa acgaccgcgg tcgaccatta
     6661 cacgtatcga ttcgcgcgca gagccgaagc agagggactc gtggtgatcg acgatccgga
     6721 gtcgattctc cgatgcacca acaaggtgta ccaagctgag ctcttcgagc agaacggcgt
     6781 cgactgtcca aagacggtcg tggtcgatcg cgacagcgcc cacctggtgg ccgaaagact
     6841 cggcctcccc tgcgttctca agaagcccga cagctcgttt tcggccgggg tggtgaaggc
     6901 caacaacgcc agcgagctgg ccgcccacct gaagcaattc ctccaggagt ccgagttggt
     6961 catcgcgcag gagttcgtcg cctccgagtt cgactggcga attggcgtca tcgatcgaaa
     7021 gcccctctat gcctgccgat accacatggc tcgcgggcac tggcagattc agaaggccac
     7081 gggcgagagc gagcgcctct acggcaagac ggatacgatc gcgatcgagg atgcgccgaa
     7141 ggcgatcgtc gatttggccg tccgtgcgtc cgggctcatc ggggatggac tctatggggt
     7201 cgacatcaag gaagtcgacg gacggctcct cgtcatggaa gtcaacgaca atccgagcat
     7261 cgattcgggt accgaggacg agattctcaa ggacgatctg taccttacga tcatgcaaag
     7321 catgtacgac cgcctcgaac ggcgagggcg ccaatgacgg cttcgacgac attgcacctg
     7381 ttcgatgctt tcgggatcga gatcgaatac atgatcgtcg atgccaagac gctttcggtg
     7441 cgcccgatcg cggacgagct cttgaaaaca gtagccggcg actacgagat ggaggtcgat
     7501 cgcggtgccc tgcgatggtc caacgagctc gcgctgcatc tcatcgagct aaaaacgaac
     7561 ggcccggtcg cgagcctcgc gaatgtgcac gagagcttcc agcgagacat cggggccatc
     7621 gatgacttgc tccgtcccat gggcgcacga ctgctgccaa gtgccatgca tccctggatg
     7681 ggcaccgacg agcttcggct ctggccccac gaggatgacg ccatctacgc agcgctcgac
     7741 cgcgttttcg actgccgcgg gcacggctgg tcaaacctgc agagcatgca catcaacctt
     7801 ccattcgcgg atgatacgga gctcgagcgc ctgcacgcgg cgatccgctt gatgcttccg
     7861 attctcccgt ccttggcagc cagctccccc ttcgtcgatg gggcagcgag cggttatctc
     7921 gacacgcggc tcgaggtcta tcgacacaac gcgaaacgta tcccgtccgt gagcggtcga
     7981 gtgagtcccg aggccgtctt cagcaaaagc gactacgagg cgcttttgga gtcgatctac
     8041 cgtgatctgg cccctctcga tccggacggc acgctgcagc acgaatggat caacgcgcgc
     8101 ggttgcatcg cccgcttcga tcggatggcc ctcgagattc gtctcatcga cgtgcaggag
     8161 tgcccacgca tggatctggc gatcgcaggg gcggctctcg cggtcacgca ggcgctcgtc
     8221 gaggagacct gggcaccact tgccgaccag cgcgcctggc atgagacgga gctcgagaag
     8281 atcctgcttt cgacgatcgc agatgcagac catgcgacgc tcgacgctcc gcgattcctc
     8341 gaggcattcg gtctcaccgg gacacggtcg atccgggcag gtgagctctg gcagcacttg
     8401 atcgagaccc aggtagaaac gcggccggag tacgcagccg ccggggacgc cttgcgcatc
     8461 atttcgcgcg aaggctgctt ggcgcgccgg atcatgcgtg cgtcgggtcc gaatccgagt
     8521 ccggagtcgc ttcgtgaggt ctatctccag ctggccgact gcttggctcg tggtcaggcg
     8581 ttttcgagcg ctccatgaag gggtacgcgc tcgtgctgag ctgcgagcat ggtggcgcga
     8641 gtgtccccca aaggtatcgg cacttgttcg acagcaagcc cgctcgtgat gcgctgaggg
     8701 gtcatcgagg aagcgatctc ggcgcacttc gtctggctcg ctcgctcgag cgccgccatc
     8761 gagtcccgct ctatgccagc acggtcaccc gcctcctcgt cgacttgaac cgctcgctcg
     8821 gacatcccca actcttctcc gagtttacgc gagacctcga tcccgcggag cgtgagagct
     8881 tgctcgatcg gcactacttc ccgcatcgaa cggcggttga atcttggatc gatgctcgag
     8941 tccgcggtgg tgatcaggtc ttgcacctcg gcgtgcacag cttcgtgccg cagatcgacg
     9001 gtcgggcccg aacggcggac atcgggctcc tctacgatcc atcgcgatct cccgagcgaa
     9061 gattttgtga tcggtggaag agctcgttgc acgagatcgc ccctgagctc cgcgtccgcc
     9121 ggaactaccc gtacctaggc aaatccgacg gattcacgac gcatcttcgt cgagcgctcg
     9181 gctcaccgca gtacctgggc atcgagctcg aggtcaatca gcgggccctt cgcacagacg
     9241 agaaccagcg cttcctcgca agggtgatct ccgacagcct gcgcgcggtg ctccgtccat
     9301 agaatcgtcg agagaatgcg atgcgcgggt tcccggacta gggtcgaatc cggccccaaa
     9361 atcgggggcg gaacctcaac gctctgcgag accggagatc gcacggggag cggctggctt
     9421 gcggcggagg ccagtttgcg ggaaacccgc ggtcgctccc ttctactttc ctgagagaag
     9481 catcacgatg acccagaagc cgaccattca gtatacgaag accgacgaag cacccctcct
     9541 ggcgacctat tcactgttgc cgatcctgag ggcgttcacc gcgcccgctg gcatcacggt
     9601 cgagcttccc gacatctcgc ttgctggacg aatactggcg agctttccgg aggcgctcaa
     9661 aaaggaacag cggatccccg acgctctggc ggagctcgga gatcttgcca agacgccgga
     9721 agccaacatc atcaagctgc cgaacatcag cgcttcggta ccccagatga aggccgccgt
     9781 agccgagctg caggccaagg gctacgcggt tccggactac cccgaagagc ccaagaccga
     9841 agaagagcgg gcgatcgccg aacgttacgg caaggtcaag gggagcgccg tgaacccggt
     9901 tctgcgcgag ggcaactcgg accgccgcgc gcccaaagcc gtaaaggaat acgcgcgcgc
     9961 caatccgcac tcgatgggag cctgggcgcg cgattccaaa acacatgttt cgacgatgag
    10021 cactggagat tttttccaca acgagaagtc gatcacgcta ccggctgcta ccaccgtgcg
    10081 catcgagcac gtcgcgaagg atgggtcgac gacggttctc aaggacggcc tcgcgctgct
    10141 tcaaggcgag atcttggacg gtaccttcat gagcaaggct gcgctcctca ccttcctccg
    10201 cgagcaggtt tgtgatgcgg tcggtctcgg cgtcctcttt tcgctccaca tgaaggcgac
    10261 gatgatgaag gtcagcgatc cgatcatctt cgggcacgcg gttcgcgtgt attttgccaa
    10321 agtattcgag aaacacgggg aaacgctggc ggctctcgga gccaatccca acgacggatt
    10381 cggagaggtg ctggcgcgta tcgaaagctt gccggccgat caacgtgccg agatcgaagc
    10441 cgatatcgcg gccgcctacg agaagggccc ggcgctagcg atggtcaact ccgacaaggg
    10501 catcaccaac cttcatgtgc cgagcgacgt catcgtcgac gcatcgatgc cggccatgat
    10561 ccgcacttct ggccagatgt ggaacgctgc gggcgacacc caggacacca aggccgtgat
    10621 ccccgacagc agctacgctg gcgtctaccg ggaggtgatc gacttctgca aggagcacgg
    10681 ggccttcgat ccgacgacca tgggcagcgt tcccaacgtc ggtctcatgg cgcagaaggc
    10741 cgaggaatac ggatcccacg acaagacctt cgagatccca agtgccggcc gtgtgcgcgt
    10801 ggtcgacgcg aagggcaatg tgctcatgga gcatgaggtt gaagcgggtg acatttggag
    10861 agcctgccag accaaagacg cggcagtacg ggactgggtc ggcctcgcgg ttcgccgcgc
    10921 gcgcgcaacc ggggcgcccg cggtcttctg gctggacgcg aagcgcgccc atgacgcaca
    10981 gctcatcgcg aaggtgaagg catacctccc cgagcacgac accgcgggct tggacatccg
    11041 gatcatggag cctgcggccg ccacacgctt ctcactcgag cgcatcagcg agggcaagga
    11101 cgcgatctcc gtgacgggca atgttttgcg cgactacctt accgacctct ttccgatcct
    11161 cgaggtcggc accagcgcga aaatgctatc gatcgtgcct ctcatgaacg gcggcgggct
    11221 cttcgagacc ggagcgggcg gctcggcgcc caaacacgtt caacagttcg aaaaagagaa
    11281 ccatctgcgt tgggactcgc tcggcgagtt cctcgcgcta gccgcgtcac tcgacttttt
    11341 gtctgagaca cacgacgacc ccaaggtgaa ggtccttgca acgacgctgg acgctgcagc
    11401 gaccaagtac ctgctcgaga acaagtcacc gtcgcgcaaa gtgaaccaga tcgacaaccg
    11461 ggggagccac ttctacctcg ctctctactg ggcgcaggcg cttgccaaat gcgacgatcc
    11521 ggagctgcgc gcctacttcg agcccatcgc caaggcattg gcagacaacg aaaacaagat
    11581 ctccgaagag ctcctcgcgg tgcaaggcaa gcccgtcgac atcgggggct actacctacc
    11641 ggacgatgct ctcgcttccc aagcgatgcg cccaagcccc acattgaacg cgatcatcga
    11701 cgcgctctga gggctccgag cgcccgcgat cggagccctt agcgcgccca cttgagcagg
    11761 aaatcgatca ggcgattctg cttccccgtg tagggcgggt agaacaggtg caggagcgag
    11821 agccgcgggt tggtgaagac cggtcgaatc ttgctgaact ccacgaagcc ctcatggccg
    11881 tggtattgcc ccattccgct cgctccaaca ccgccgaagg gtaggtcgtg ttgggcgatg
    11941 tgcatgatgc aattgttgat cgtcacgcca cccgagatcg tgctgtaaag aagcttttcc
    12001 tgtcgcgaac ggtcgttggt gaagacgtag aaaccgagcg gccgatccct cttggtgacg
    12061 tagtcgatcg actcgtcgag atcccgatac gtcatcacgg ggaagagcgg accgaagatc
    12121 tcctcctgca tgatcatcat gtcatcggtg acgtcgagca ccagatgtgg cgggatcttg
    12181 cgcaacgcgt cgttgaaaga agctccaggc acgaggggta ccagcttcgc gcccttggcc
    12241 tctgcgtcat cgaggttcat gcgcagtcgg cgatacgact tgtcgtcgat taccgacgtg
    12301 taactgccgt cgttcgtatc cgggtagcgc tccgcgacga tacgcttggc cgactccacg
    12361 aaccggtccc gccccgcctc gggcaagaag agatagtctg gtgcgaggca ggtctggccc
    12421 gcgttgatga acttggcata gaggattcgc gaggccgctt cgtcgatgtc gaaatcatca
    12481 cagacgatcg tgggtgattt tccaccgagc tcgagcgtca ccggcgtgag gctttccgcc
    12541 gcggatcgca tgacggttcg tccggcgtcc gcggagccgg tgaaaatgat gtggtcgtag
    12601 ggcagcgaag agaagtcgcg gccaggtacg cccggcagaa aggcgagggt gtcctcgggg
    12661 aaaacctcgc tggccttctc ctgaagcagg cggcaaaggg tttgggagtt gctggccatc
    12721 ttgatcatgg cgcggttccc tgcggccaag acgctgatca gcgggctcac cgtgagaaaa
    12781 agcggatagt tccacggcga gacgatccct acgacgccct tgggctgcgg gatgacccgg
    12841 ttcgagccgg tcgcgaacag cagcgaaacc tcgcggcgtt gtggacgcat ccatttccgg
    12901 agcttcttcc gggcatgccg gattccatcg acacagggaa agatctcgag gatcttcgat
    12961 tcctcggcgc agcgatgtcc gaagtcctcc cggatggctg cgacgatcgc atcgctgttg
    13021 tcgagcagaa tccgctcgag cgcggtcagt cgttctgcgc gaacctcgcg gcttgggtag
    13081 gggtccctga ggtatgcatc gcgctgcagc ttgaagatgc gttgcgcttc gttttcgacg
    13141 atctcgatgc ggcggagttg agcggtttga gccatatgga ctgctggtgt agcgcctcgg
    13201 gacggtcgag cgcaagcaag gcctgtcagc gttccagcgc ctcatgtatt gcggtcacgc
    13261 aagccgttgg ctgaaagtcc ggcagaatgc gggcgagctt gctgccatcg atcccgtggg
    13321 ggacttccca taagtgtcgc atttcgacga cttcacgcat gggcgcgctg aaagcgccca
    13381 tcagtcgaac cagtccccag ggaaagcgct tgaccttcaa cgggcgctcg agggcgcgct
    13441 ccatggtttc gacgagctcg gccccggtaa gcgaaaagcc accgaagccg atcgtttcga
    13501 aggcgccgag gctttcgcgg accgccgcca ggccaaccat cgcccgagcg agatccggca
    13561 agtaggccca ggcgtggacg cgatcgagag gccccggata cgtcacgatc cctttgtcga
    13621 ccttgttggc gacataggta tcgaaccagt tgcccgtcgc cttgcgctcg atgaagtccc
    13681 cggcgcgaag cacgatcgtc tgtatgccgc gcgcgctcgc ctgccggtaa gcctcttcga
    13741 tctcttcgcg tagtcgccct ttgcgggtcg tcgggaggtg cggcgtgtct tcgcgcagaa
    13801 cgggaggcat gccttccccg tagttgtata cgttgcccgg tagcatcacc gtagcgccgc
    13861 tgccctcggc agcgctcagg atgctccgcg tgatcttagg aacttcgtcc gcccagcgtt
    13921 cgtagggagg gttgagcgcg ttcacgatga ccgcgacgcc ctcggacgcc gccgtaacgg
    13981 cttcagcgtc gaacgcatcc ccctcgaata cggttactcc gtcccgtacg cgcgcggaag
    14041 aaccggtgcg cacgaaggcc cgcaccttcc atcccgcgtc caagaacgcc tccaccgacc
    14101 agcgtccgat gcgaccattg gcgcccacga ccagtacttc tttttccatg ctgtcctgct
    14161 ccttgccgca gtccgctgcc gttcggtttg aatcatacgc atgcaaaaat gatggtaaat
    14221 acgccaaata tagaatatag atatacaaga atgaatagct gcccagatgc ctgctgagtt
    14281 cgactgg
//
LOCUS       DASRZF010000238         4052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2574922, whole genome shotgun sequence.
ACCESSION   DASRZF010000238 DASRZF010000000
VERSION     DASRZF010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2574922"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VFG22_10490"
     CDS             complement(<1..244)
                     /locus_tag="VFG22_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416711.1"
                     /translation="MSNNEPPDEWHEIVSLIDDAGETLPTSDPHPQVWVRGSIGEITI
                     VQVEHPGDIQGVSRLLKDAGIEKTLIVPRSVRFMRLR"
     gene            356..541
                     /locus_tag="VFG22_10495"
     CDS             356..541
                     /locus_tag="VFG22_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416712.1"
                     /translation="MSYDWRNDPASYYEWERRNRALIIVPAAPIETDYEKARKYEPAL
                     WDIDSDIGPAKGPARIN"
     gene            544..1005
                     /locus_tag="VFG22_10500"
     CDS             544..1005
                     /locus_tag="VFG22_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416713.1"
                     /translation="MESAFKIGDKVMFGRPNGEQTHGIVRKVNGKTILVEQAEARGRE
                     RIREAGAKWRVAASLVRHAVPRQYVVGGVLIESDSMGEPPSHPLRGLVPPLVKRSKEA
                     IEADIDSVESQLSPENLFWDGERPRGEALAAGRILNRRLRALEAELLSTLA"
     gene            1056..1376
                     /locus_tag="VFG22_10505"
     CDS             1056..1376
                     /locus_tag="VFG22_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416714.1"
                     /translation="MNGYTYCACRDCMEIAIGESGKALCHACEDAGCELGSECGAEGN
                     YNVLDNHDDERDMGEDEAEDGPEEEPPVCPLDGGDGIPLGGLGNLMHYRCRACGMNFS
                     HTKE"
     gene            1380..1718
                     /locus_tag="VFG22_10510"
     CDS             1380..1718
                     /locus_tag="VFG22_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416715.1"
                     /translation="MTMATLYYVSSHATRDERVAARDDAALLPKGKYDVTARIDVGDI
                     GTSPADCISAADALFERFNIGDRAGLSVRSMSVGDVVAFDGRAIAMLCCKVGWRAVPA
                     SRLPEPTKAS"
     gene            1765..2478
                     /locus_tag="VFG22_10515"
     CDS             1765..2478
                     /locus_tag="VFG22_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416716.1"
                     /translation="MTSVRTESDAVACLATLGVAVSALKLETAADGSSIVVESYSCGR
                     CGGHGHLGRGTCDVPWGVCYRCFNGPKRAGISRTPLIEYARKAKATVARRKSAAAKRE
                     AKDNARVERRLEGERKWCEANGHGRVTFAERDAKRAAEWAARDAKKVHVGVVGERSVF
                     TATVKAIPSWDSAYGTVYMVIMEDAAGNSLVWKTGSPCLEVGETATFKASVKEHGDYK
                     GVARTLVTRVKPEPIAEAA"
     gene            2475..2717
                     /locus_tag="VFG22_10520"
     CDS             2475..2717
                     /locus_tag="VFG22_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416717.1"
                     /translation="MSEEAEIVVEVERVRCGMHNSRLGAARITSGEPYVLAGFEGTDL
                     PSLATELAETGHVFRVEIVERFVSMLEWYGCESEVG"
     gene            2717..3235
                     /locus_tag="VFG22_10525"
     CDS             2717..3235
                     /locus_tag="VFG22_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416718.1"
                     /translation="MFISSGRGGYTRSGEQRASGRFLMLFCVKPGGELRVLVRHVRMR
                     QCGHWMMANVTVKSAGAREAAESEPAYKSNYWRRRVFDGRFEIGLSGTYGCDGLPISA
                     DDYPGLWDQLHPVPAELTEACWKGGGHNSAGSEGPAMFEWAKSNAVELSRLRVSSGSA
                     DTSTGRDEAVTT"
     gene            3282..3620
                     /locus_tag="VFG22_10530"
     CDS             3282..3620
                     /locus_tag="VFG22_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416719.1"
                     /translation="MIHDKRAFNIVTVRSAAALAKKLSEHTWTLCSGFSHGGYLFLND
                     SFSEDGAAEFAVVRESDALQVESITFGWCNVEESLGYIKRAVAGEFDAAGHSKIELLN
                     HGDYRCYHCA"
     gene            3631..3831
                     /locus_tag="VFG22_10535"
     CDS             3631..3831
                     /locus_tag="VFG22_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416720.1"
                     /translation="MDDLRTAFAEADRLTKHTAREHIVRNRGFAHKPDYIVVPRYEAG
                     FEGYRHDGGYAGLGLWVTERPS"
     gene            3828..>4052
                     /locus_tag="VFG22_10540"
     CDS             3828..>4052
                     /locus_tag="VFG22_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416721.1"
                     /translation="MNRPFYQYLAGALTARENCKKAGNAEWLDRWESRLEQLDKLLPS
                     GSGFDAGSVVVKGESSSRQIVIATSFHHMDE"
BASE COUNT          801 a         1178 c         1365 g          708 t
ORIGIN      
        1 gtcgcaggcg catgaagcgg acgctgcgag gcacgatgag cgttttctcg attccggcat
       61 ctttcagcaa gcgagacacg ccctggatgt cgccaggatg ttcgacctgg acaatggtga
      121 tctctccgat cgaacccctg acccagacct gtgggtgcgg atctgacgtt gggagcgttt
      181 ctccggcgtc gtcgatcaga gagacgattt cgtgccactc gtcgggcggt tcgttgttcg
      241 acatgccgcg aaatatgtgg aggttgacag cgtttgtcaa cggcgaacgg tgccgtggac
      301 ggctacttga caaaccgacc tggtaccccc atattggctg tgaaaggaac cccaaatgag
      361 ctacgactgg cgaaacgacc ctgcttccta ctacgagtgg gagcgtcgaa accgcgcctt
      421 gatcatcgtc cctgccgcgc cgattgagac cgactatgaa aaggctcgca agtacgagcc
      481 cgcgctctgg gacatcgact ccgacatcgg ccccgcgaag ggccccgcaa ggatcaactg
      541 accatggaaa gcgctttcaa gattggggac aaggtaatgt tcggtcggcc caacggcgag
      601 caaacccacg gaatcgtccg caaggtcaac ggcaagacga ttcttgtcga gcaagccgag
      661 gcccgaggac gggagcggat ccgagaggcg ggggccaagt ggcgggtcgc ggcgtcgctg
      721 gttcgtcatg cggttccgcg tcagtacgtt gtgggcgggg tgttgatcga gtcagattcc
      781 atgggagaac ccccttcgca cccgcttcga ggtcttgtgc ctcctttggt gaagcggtcc
      841 aaggaagcaa tcgaggcgga catcgactcc gtggagtcac aactttcgcc cgagaacctg
      901 ttctgggatg gcgagcgccc acgtggtgag gctctggcag ctggccgaat cctgaatcgc
      961 cggttgcgag cacttgaggc cgagctgctg agcacgctgg cttgacaaac cggatagata
     1021 ccattagatt gaatcgtgga aggagaacgt tgaccatgaa tgggtacaca tactgtgcgt
     1081 gtagggactg catggaaatc gccatcggcg aatctggcaa ggcgctgtgt cacgcctgcg
     1141 aggacgcggg ttgcgagctt ggcagcgaat gcggggccga gggcaactac aacgtgctcg
     1201 acaaccatga cgacgagcgt gacatgggcg aggacgaggc cgaggacggg cccgaggagg
     1261 agccgcccgt ctgcccgctc gacgggggcg acggcatccc gctcggcgga ctcggcaacc
     1321 tcatgcacta ccgctgccgc gcctgcggca tgaacttctc tcacaccaag gagtgaacga
     1381 tgacaatggc aaccctttac tacgtctcca gtcacgcgac ccgagatgag cgcgtggcgg
     1441 cccgagatga cgccgcgctc ctgccgaagg gcaaatacga cgtcaccgcg cgcatcgacg
     1501 ttggggacat cggaacgagc ccggctgact gcatttcggc tgccgatgca ctgttcgagc
     1561 ggttcaacat cggtgaccgc gccggtctgt ccgtgaggtc gatgtcggtc ggagacgtcg
     1621 tcgccttcga cggtcgggca atcgcgatgc tgtgctgcaa ggtcggctgg cgggcggtac
     1681 cggcatcccg tctgccggag ccaacgaagg cttcttgaca gacgcatctg ttggcactat
     1741 cttgattctc ggaaggaaag aaaaatgacc tcggttcgca cagagtctga cgcggttgcc
     1801 tgcctggcga cgctcggcgt tgcggtgagc gcgctgaagt tggagaccgc cgccgacggt
     1861 agctcgatag ttgtcgagtc gtattcctgc ggacgctgcg gcggccacgg tcacctcggc
     1921 cgcggcactt gcgacgttcc ctggggcgtc tgctatcggt gcttcaacgg cccgaagcgc
     1981 gcgggcatca gccgcacgcc gctgattgaa tacgctcgca aggccaaggc gactgtggcg
     2041 cggcgcaaga gcgcagcggc caagcgagag gccaaggaca acgcccgcgt ggagcggcga
     2101 ctcgaagggg agcgaaagtg gtgcgaggcc aacggccacg gtcgtgtgac cttcgcggag
     2161 cgcgacgcca agcgagctgc cgagtgggcc gcccgagacg ccaagaaggt ccacgtcgga
     2221 gtggtcggcg aacgctcggt cttcaccgcc acggtcaagg ccatcccgag ctgggacagc
     2281 gcctacggca cggtctacat ggtcatcatg gaggacgcgg ccggaaactc cctggtctgg
     2341 aagaccggga gcccgtgcct ggaggtcggc gagacggcga ctttcaaggc cagcgtcaag
     2401 gaacacggcg actacaaggg tgtcgcccgg accctggtca cccgagtgaa gcctgagccg
     2461 attgcggagg ccgcatgagc gaagaagcag aaatcgtagt cgaggtcgag cgcgtgcggt
     2521 gcgggatgca caactccagg ctcggagcgg ctcgcatcac aagtggcgag ccttacgtgc
     2581 tcgcgggctt cgagggcacc gacctgccga gcctggccac agagctggct gagaccggcc
     2641 atgtgtttcg ggtcgaaatc gtcgagcgct tcgtctcgat gttggaatgg tacggatgcg
     2701 aaagtgaggt tggctgatgt tcatttcctc tggtcgtggt gggtacacca ggtctggtga
     2761 gcagcgcgca agcgggcgct tcctgatgct gttctgcgtc aagccgggcg gcgaactgcg
     2821 cgtgctcgtg cggcacgtcc ggatgcgcca atgcggccac tggatgatgg ccaacgtcac
     2881 ggtcaagtcc gcgggcgcgc gcgaggcggc cgagtctgag cccgcgtaca agtcgaacta
     2941 ctggcgacgt cgagtgttcg acggccggtt cgagatcggc ctgtccggaa cctacggctg
     3001 cgacggcttg ccgatcagcg ccgacgacta cccaggtctc tgggatcagc tgcatcccgt
     3061 accggccgag ctgaccgagg cgtgctggaa gggcggcgga cacaacagcg cgggctccga
     3121 gggcccggcc atgttcgagt gggccaagtc gaacgccgta gagctttcga ggctgcgtgt
     3181 cagttcgggg agcgccgata cgtctactgg tcgagacgag gcagtgacca cttgacaaac
     3241 gcatggtcga taatcagatt ggtagcgaaa ggcgacatct catgattcac gacaagcgag
     3301 cgttcaacat tgtgactgtc aggtctgcgg ccgcgctggc caagaagctc tccgagcata
     3361 cgtggacgct ttgctctggc ttcagccacg gcggctatct gtttctcaac gactcgttca
     3421 gcgaggacgg cgcagccgag ttcgcggtcg tgcgcgagtc cgacgctctc caagtcgaga
     3481 gcatcacgtt cggctggtgc aacgtcgagg agtcgctcgg ctacatcaag cgagcggtgg
     3541 ccggtgagtt cgacgcggca gggcacagca agatcgagct gctgaatcac ggcgactacc
     3601 gttgctacca ctgtgcatga aggataactc atggacgatt tgagaacagc gtttgccgag
     3661 gccgacaggc tgaccaagca cactgctcgc gaacacatcg ttcgcaatcg agggttcgcg
     3721 cacaagccgg actacatcgt ggtgcctcgc tacgaggcgg gattcgaggg ctaccgccat
     3781 gacggcgggt atgccggact gggcctttgg gtcacggaga gaccatcatg aacagaccat
     3841 tttaccaata cctcgccggg gccctgaccg cgcgcgagaa ctgcaagaag gctggcaacg
     3901 ccgaatggct cgatcgctgg gagtcgaggc ttgaacagct cgacaagctc ttgccgagcg
     3961 gcagcgggtt cgatgccggg tcggtggttg tgaagggcga gtccagtagc cgccagatcg
     4021 tcatcgcgac ctcgttccac cacatggacg aa
//
LOCUS       DASRZF010000239         3557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3265695, whole genome shotgun sequence.
ACCESSION   DASRZF010000239 DASRZF010000000
VERSION     DASRZF010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3265695"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..643
                     /locus_tag="VFG22_10545"
     CDS             182..643
                     /locus_tag="VFG22_10545"
                     /inference="COORDINATES: protein motif:HMM:NF013811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET6416722.1"
                     /translation="MPTSLEADLAVTAAVVDHFAEKTPDVVFVHLDEVDHQGHVAGYS
                     AMVPEYLDAIEMIDAQIGEMLDALRARDTYAMEDWLVIVTTDHGGLGRGHGGQSAEER
                     TIFLIASGPSVSSGQVISPGPGQTAVPATVMKHLGLSVDPVWGWESEAFGL"
     gene            complement(650..1423)
                     /locus_tag="VFG22_10550"
     CDS             complement(650..1423)
                     /locus_tag="VFG22_10550"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6416723.1"
                     /translation="MRPMGFEVAPDGYDAFIGRYSRELAPRFADFAEVVAGPALDVGC
                     GPGALTLELAARVGSDEVAGVDLSERFVEACRARVPGADIRLASAESLPFATGSFASA
                     LSQLVVSFVGNPSKMASEMARVVHAGGTVALCMFEANGIAMLGAFWEAARRFDPRAPD
                     DARMPFRTSAELVQLCERAHLRDLVTTEISIEASYSSFDELWSPFSHGIGPAAEYLQA
                     QDPARRLAIREAYFDLLGRPADAFVLPAKVIAVRGRTAD"
     gene            complement(1503..2411)
                     /locus_tag="VFG22_10555"
     CDS             complement(1503..2411)
                     /locus_tag="VFG22_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416724.1"
                     /translation="MKTQFLFALSACMAFVFQLGCSSDATVDPSTQNLTPACIENAAD
                     LDGEDWLCPNDLTVECEDGAADPALIYLEPSGDLPESCDEIELSINDEGPFELGTHDI
                     VVTAEASGSDAGTVEVSCEATLTVVDTVPPEASDEVIELWPPNHKFHAISGEDCVRDA
                     CDGDLAVTFLSASSDEPVNDKGDGNTEPDIILDCDQVQLRSERQGGSNGRVYTLGWVA
                     VDDAGNETEGECIVTVPHDQSGRAAVDDGAAYQITLDSDECDHGAGGSGGQGGEGGMG
                     GSAGEGGAGGMGGSAGEGGAGGVLVE"
     gene            complement(2595..>3557)
                     /locus_tag="VFG22_10560"
     CDS             complement(2595..>3557)
                     /locus_tag="VFG22_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416725.1"
                     /translation="FITSLVLVLGFSAGACGGDPTGENRGGGGTAGMGAGGTGVVGGN
                     GGVSGSTGTGGTAGTGGTTGTGGTAGTSGACFPVLCPDGNSYGCGDCFDNDGDGLVDD
                     KDLECLGPCDNTEGPVLLTGTPGETGNQCGADCYFDRGNGSGSGECKWDRRCDPFGPK
                     DGCVYNPALIGAQNYCPDVQPELCQQDCGALTPNGCDCFGCCTFPELNGGYVFLGSYV
                     GNDGTCTLNDLDDPALCRPCTPAGNCLNTCERCEICLGKPTIPADCFPGTGGSGGMGG
                     GGVGGNGGGLRCPVGKQACGLPGDPACAVGAFCLTGCCTEIVVQ"
BASE COUNT          679 a         1156 c         1100 g          622 t
ORIGIN      
        1 gccttcgctg gtggcgaacc gggcaccccg accgagcaag cgacgttcag cggccccggt
       61 tggagcagca tcctcactgg ggtctgggtc gacaagcatg gagtaaccga caacagcttc
      121 gatggtgcgc gattcgacga gtatccgcat ttctttgcac gcgtccgaga ggagaaccct
      181 gatgccaacc tcgctcgaag ccgatctcgc cgtgaccgca gcggtggtcg accacttcgc
      241 agaaaagacg cccgacgtgg tgttcgttca cctggatgaa gtcgaccatc agggccacgt
      301 cgcaggctac tccgcgatgg ttccggagta tctcgatgcg atcgagatga tcgatgccca
      361 gatcggcgag atgctggacg cgctccgggc gcgggacacc tacgcaatgg aagactggct
      421 cgtcatcgtg acgacggacc acggcggtct cggccggggg cacggcgggc aaagcgcgga
      481 agagcgcacg atcttcctca tcgcgagcgg cccgtcggtt tcgagtgggc aagtgatttc
      541 cccgggaccc ggacaaaccg ccgtgccagc cacggtgatg aagcacctcg gcctcagcgt
      601 tgaccctgtg tggggttggg aaagcgaggc gttcggcctc tagaagcgat caatcggccg
      661 tccggccacg gaccgcgatc accttggcag ggagcacgaa ggcgtcggcg ggtctgccga
      721 gcagatcgaa gtaggcctcg cgaatcgcaa ggcggcgcgc tgggtcttgg gcctgcaagt
      781 actcggctgc tgggccgatt ccatgcgaga acggcgacca gagctcgtcg aagctcgagt
      841 acgacgcttc gatggagatc tcagttgtca ccaagtcgcg cagatgcgct cgctcacaaa
      901 gctgcacgag ctctgccgat gttcggaacg gcatgcgcgc atcgtcgggg gcacgaggat
      961 cgaaacgacg tgcggcttcc cagaacgcac ccagcattgc gatcccgttt gcttcgaaca
     1021 tgcataaagc cacggtgcca ccggcgtgca cgactcgagc catctccgat gccatcttcg
     1081 atgggttgcc gacgaaggaa acgaccaact gtgaaagcgc ggaggcaaag ctgcccgtag
     1141 caaagggcag actctcggcc gaggcgagtc ggatgtcggc cccagggacc ctagcgcggc
     1201 aagcttccac gaaccgctcc gacaaatcga cgccggcaac ctcatcagaa ccgacacgcg
     1261 ccgcgagctc gagcgtgaga gcgcctggcc cgcagcctac gtcgagcgcc ggccctgcga
     1321 ccacctccgc gaagtctgcg aaacggggtg cgagctcacg cgagtaccgt ccgatgaacg
     1381 cgtcgtagcc gtcaggcgca acctcgaatc ccatcgggcg cattgagcac ccgatgaggt
     1441 tcaggtcaag gtgccggccg agtccttgga aaggacgtta ggcgagcctg cctcgggttc
     1501 tgctattcga cgaggacgcc accggcgccg ccttcaccgg ctgagccgcc catgccaccg
     1561 gcgccgcctt caccggctga gccacccatg ccgccttcgc cgccttggcc gcctgaaccg
     1621 ccggcaccat ggtcgcattc atcggagtcg agcgtgatct ggtaggccgc gccgtcatcg
     1681 accgccgctc tgccgctttg atcatgcgga acggtaacga tgcactcgcc ctcggtttcg
     1741 tttccggcgt catccaccgc tacccagcca agcgtgtaca cgcgaccatt gctgccaccc
     1801 tgccgctccg agcgtagttg cacttgatcg cagtcgagga tgatatcggg ctcggtgttt
     1861 ccatcgccct tgtcgttgac cggctcgtca ctcgatgccg agagaaaggt aacggcgaga
     1921 tcgccatcgc acgcgtccct cacgcaatct tctcctgaga tggcgtggaa cttgtgattg
     1981 ggaggccaga gctcgatcac ctcgtcgctt gcctcgggag gcactgtgtc gacgacggtc
     2041 agcgtagctt cgcagctcac ttcaacggtt cctgcatcgc tgcccgaagc ctccgcggtg
     2101 accacgatat cgtgcgtgcc aagctcgaag ggaccttcgt cgttgatcga gagctcgatc
     2161 tcgtcgcagc tttcgggaag atcgccggat ggctcgagat agatgagcgc cgggtctgcg
     2221 gcgccgtcct cacactcgac cgtcaaatcg ttggggcaaa gccagtcctc gccatcgagg
     2281 tccgcggcgt tttcgatgca cgccggagtg aggttctggg tcgacgggtc gaccgtcgcg
     2341 tcactggagc aaccgagttg gaacacgaag gccatacagg ccgagagcgc gaaaagaaat
     2401 tgggtcttca tagttcacta catcgcaccg ctcatgcgtg atgccaagct atgtgcctca
     2461 caaactacga aagaatacac gttccaacca gactacatta cctacatcac agtatgtgag
     2521 gcggatcgca gaacgcgctt gcgacttgcg tggattgggg ctttgcatcg cgattcgatc
     2581 gcggaacaag ggcgctactg gacgacgatc tcggtgcagc agcccgtcag gcagaaggcg
     2641 cccacggcgc aagccggatc gcccggtaga ccgcaagcct gctttccgac cgggcaacgt
     2701 agaccgccgc cgttcccacc cacgccgccg ccacccatgc cgccgcttcc gcctgtacct
     2761 gggaagcagt ctgctgggat ggttggcttg ccgagacaga tctcgcagcg ctcacaggtg
     2821 ttcaagcagt tgccagccgg cgtgcaaggt cggcagagcg ccgggtcgtc gagatcgttt
     2881 aaggtgcagg tgccgtcgtt cccgacatag gagccgagga agacgtaccc gccattcagt
     2941 tcggggaacg tgcagcatcc aaagcagtca cagccgttcg gcgtgagcgc cccgcagtct
     3001 tgctggcaaa gctcgggctg cacgtcgggg cagtaattct gagcaccaat gagagcgggg
     3061 ttgtaaacgc agccatcttt gggcccgaac ggatcgcagc gtcgatccca tttgcactcg
     3121 ccggatcccg atccattgcc ccggtcgaag tagcagtctg caccgcactg attgccggtc
     3181 tcgccggggg tgccggtcag aaggacgggc ccttctgtgt tgtcgcaagg cccaaggcat
     3241 tcgagatctt tgtcgtcgac gaggccgtcg ccgtcgttgt cgaagcagtc accacagccg
     3301 taggagttgc cgtccggaca aagaaccggg aagcaggctc cgctcgttcc cgcggtcccg
     3361 cctgtacccg tcgtgccgcc ggtccccgcc gtgccgccgg tcccggtcga gccgctcact
     3421 ccgccgttgc cccccacgac accagtgccg cctgcgccca ttcccgcggt cccgccgcct
     3481 ccccggtttt caccggttgg gtctccgccg catgcgccgg cagaaaatcc aagaaccaaa
     3541 accaatgaag ttataaa
//
LOCUS       DASRZF010000240         3861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_514565, whole genome shotgun sequence.
ACCESSION   DASRZF010000240 DASRZF010000000
VERSION     DASRZF010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514565"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="VFG22_10565"
     CDS             <1..1059
                     /locus_tag="VFG22_10565"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET6416726.1"
                     /translation="DYEARLHTMGKMTAVYQTVGLLAARLHTERWFEMKPDSSQIAIE
                     RPIIITGMVRTGSTALHYLMGANPDMQCLQYWLALHPQPRPPRAYWEAATDFQHARIE
                     LDMMYQAGKSMLESIHFMMPDGPDESGRILGQNFSDDRFEVVSTVPTYSDWYMNTVHR
                     ETYQRHKRIMQLIGSYEPEKRWLLKYPVHMRNLDALLEAYPDACIIWTHRDPADVLSS
                     YVSLCAHFRSLFEKDVDRPRIALEQKEAWARAVENGMRLRKGREHQFHDIYFNDFMSD
                     PLGEVAKTYAHFDQPFSERAKKALAGWREAHKPGAFGTHNYTRDDFGQSRAQIHERYA
                     EYLEQFPRVLEKRSRSAA"
     gene            1056..2360
                     /locus_tag="VFG22_10570"
     CDS             1056..2360
                     /locus_tag="VFG22_10570"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HET6416727.1"
                     /translation="MIRRSDEERALIAEAERLFPTSTRCLTFDPELNFCIGKAQGSHI
                     WDVSGNEYIDYLLGSGPHILGHAHPAVLEALRTVPEGGTSHLIINEHAIKLGQKIVEH
                     VPCAEMLSLHSSGSEATFFAMRLARVYRKRDKILKFEGAYHGMNDYALMSNQWTMGSP
                     AYPKAVPNSHGIPRALNDQVLIAPFNDLEAAAAIISKHHDELAGVIIEPVQRTFEPKP
                     GFLEGVREVTANFGIPLIFDEVVTGFRLGLGGAQALYGVTPDLCALGKAISGGLPLGV
                     LCGRRDIMEIANPKRRLQMLPYSMQTGTFSSNPVCASVALALIEELEKPGVYARLNEV
                     GTRVRAGLRDAIEDTGIEACVTGVPSVFEIWFTRDEPYDHRSSKASDFYKNIRFSDLL
                     LQEGVLKAAEKFFISAAHSDEDIERTVTAFHVALEQLAKEVR"
     gene            2357..3394
                     /locus_tag="VFG22_10575"
     CDS             2357..3394
                     /locus_tag="VFG22_10575"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6416728.1"
                     /translation="MKRVLVTGACGNIGANVVDLLVERGYSVRAVDLDTPVGRKAAAR
                     WGERVDLRFGTICNAELLRELVLGMDHVIHLAAMVPPGTDVDQAAGYAVNVVATRSLI
                     AACEAQAVPPRLTFTSTAAIWGRNDEVEGPRRADEEISPSDNYSRQKAECEAMIRASS
                     LESVIFRIAMTPPVAPGALFPFVFDMHPDMRVEFTHPKDQALGIVNSLAVHEPVVGRT
                     LCLGGGAQNRYRYREFMNMAFAAMGFPPLPASAFGDALFLTDWVDSEASQAILDYQRR
                     SAAQYFEEVSAELGPALHLTKRLGPALAPLILAHSRHQAFTEGKQPRLPNLYRAITTM
                     RRAIKAAKSYL"
     gene            3405..>3861
                     /locus_tag="VFG22_10580"
     CDS             3405..>3861
                     /locus_tag="VFG22_10580"
                     /inference="COORDINATES: protein motif:HMM:NF025050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6416729.1"
                     /translation="METSNRVLDAVLKELSVPHIEEQIPRDTFYQLGLKFPQQYGIAC
                     CDVADCVRRAEALGAGPFIHATLSPPNWVENGEPVPDCKLEVALGYAGDTQIEFLGPG
                     DGTEHYARALIETDIAFHHVGIYQQGMEQLAASITAAGYPEVVRGGISMG"
BASE COUNT          766 a         1293 c         1137 g          665 t
ORIGIN      
        1 gactacgagg cgcgtctcca cacgatgggc aagatgacgg cggtctacca gaccgtaggg
       61 ctgctggcgg cccgactaca taccgaacgc tggttcgaga tgaaaccaga ctcttcgcag
      121 atcgccatcg agcgtccgat catcatcacc ggcatggtcc gcaccggcag cacggcgctt
      181 cactacttga tgggcgccaa tcccgacatg cagtgcctgc agtattggct cgctctacac
      241 ccgcagccgc gccctccgcg cgcctactgg gaggcggcaa ccgattttca acatgcgcgc
      301 atcgagctcg acatgatgta ccaggcgggc aagagcatgc tcgagtcgat tcacttcatg
      361 atgcccgacg gccccgatga atccggacga atcctcgggc aaaacttcag cgacgatcgt
      421 ttcgaggtcg tgtcgaccgt acccacgtat tccgactggt acatgaacac cgtccatcgc
      481 gaaacctatc agcggcataa gcggatcatg cagctcatcg gctcttacga acccgagaag
      541 cgctggctcc tcaagtatcc ggtgcacatg cgcaatctcg atgcgctcct cgaagcctac
      601 ccggacgctt gcatcatctg gacgcaccga gacccggccg acgttctgtc ttcgtacgtg
      661 agcctctgcg cgcatttccg ctccctgttc gagaaggacg tcgaccgccc ccggatcgcg
      721 ctcgagcaga aagaagcctg ggcgcgtgcc gtcgagaacg ggatgcggct gcgcaaaggc
      781 cgcgagcatc agttccacga catctatttc aacgacttca tgtccgatcc gctcggcgaa
      841 gtcgccaaga cgtacgcgca tttcgaccag cctttttcag agcgcgcgaa gaaagcgctc
      901 gccggctggc gcgaggcgca caagccaggg gcgttcggca cgcacaacta cacccgcgac
      961 gatttcggcc agtcccgcgc acaaattcac gagcgctacg cggaatatct cgagcagttc
     1021 ccgcgcgtgc tcgagaagcg aagccggagc gccgcatgat tcgacgaagc gacgaggagc
     1081 gcgcgctgat cgcggaagcc gagcgactgt ttccgacgtc gacgcgctgc ctgaccttcg
     1141 accctgagct caacttctgt atcggcaagg ctcagggcag ccacatctgg gacgtgagcg
     1201 gcaacgagta catcgactac ctgctcggct ccgggccgca catcctgggt cacgcgcatc
     1261 cagcagtgct cgaagcgctc cgcaccgtac ccgagggcgg cacctcgcac ctgatcatca
     1321 acgagcacgc tatcaagctc ggccagaaga tcgtcgagca cgtgccctgc gccgagatgc
     1381 tcagccttca cagcagcgga agcgaagcga ccttcttcgc gatgcggctc gcgcgtgtct
     1441 accgtaagcg cgacaagatc ttgaaattcg aaggcgccta ccacggcatg aacgactacg
     1501 cgctgatgag caatcaatgg accatgggtt cgccggcgta tccaaaggcc gttccgaaca
     1561 gccacggcat ccctcgagcg ctgaacgacc aggttctgat cgcgcccttc aacgacttgg
     1621 aggcggccgc cgccatcatc tcgaagcacc acgacgagct cgcgggcgtc atcatcgagc
     1681 ccgtccagcg caccttcgag ccgaagcctg gtttcttgga aggcgttcgc gaggtcaccg
     1741 ccaatttcgg cattccattg atcttcgatg aagtcgtcac cggctttcgg ctgggcctcg
     1801 gtggagccca agccctctac ggagtcacgc cagacctctg cgcactcgga aaggcgatca
     1861 gcggaggcct tccgctcggc gtcctctgcg ggcgaagaga catcatggag atcgcgaacc
     1921 cgaagcgaag gcttcaaatg ctgccctact ccatgcagac cggcaccttc tcctcgaacc
     1981 ccgtctgcgc aagcgtcgcc ttggcgctga tcgaggagct cgagaagccc ggcgtctacg
     2041 ctcgcttgaa cgaggtcgga acacgggtgc gggcgggtct gcgcgacgcg atcgaggaca
     2101 ccggcatcga agcctgcgtc accggagtgc cgagcgtctt cgagatctgg ttcacccgcg
     2161 acgagcccta cgaccatcgg tcctcgaaag cttccgactt ctacaagaac atccgcttct
     2221 ccgaccttct gcttcaggaa ggcgtcctga aggctgcaga gaagttcttc atctcggcgg
     2281 cacatagcga cgaggacatc gagcgtaccg tcacggcgtt tcacgtcgcg cttgagcagc
     2341 tcgccaagga ggtccgatga agcgcgtcct ggtgaccggc gcttgcggaa acatcggcgc
     2401 taacgtcgtc gacttgctcg tcgagcgcgg ctattcggtg cgcgccgtcg acttggatac
     2461 acccgtgggc cgaaaggcgg ctgcacgctg gggcgaacgc gtcgacctgc gtttcgggac
     2521 gatctgcaat gcagagctgc tgcgcgagct ggtcctcggc atggaccacg tcatccactt
     2581 ggcggcaatg gtcccaccgg gaaccgacgt cgaccaggcc gccggctacg cggtcaacgt
     2641 cgtcgcgacg cgttccttga tcgcggcctg cgaggcgcaa gcggtgcctc cccgactgac
     2701 cttcacctcc accgcggcga tttggggacg caacgacgag gtcgagggcc cgcgtcgcgc
     2761 tgacgaagag atttcaccga gcgacaacta ctcccgtcaa aaagccgagt gcgaagccat
     2821 gattcgcgca tcgagtctcg agagcgtcat ctttcgcatc gccatgacgc caccggtcgc
     2881 gcccggcgcg ctcttcccat tcgtgttcga catgcaccca gacatgcggg tcgaattcac
     2941 ccatccgaag gatcaagccc tcggcatcgt caactcgctg gccgtgcatg aaccggtcgt
     3001 cggtcgaacg ctctgcctcg gcggaggcgc ccaaaaccgc tatcggtatc gcgagttcat
     3061 gaacatggcg ttcgcggcga tggggtttcc accgctaccc gcctcggcat ttggcgatgc
     3121 gctcttcctt accgactggg tcgattccga ggcgtcccag gcgatcctcg actaccagag
     3181 gcgcagtgcc gcccagtact tcgaagaggt cagcgcggag ctcggaccgg ccctacatct
     3241 cacgaagcgg ctcggacctg cgctcgcccc actgatcctc gcgcactcga gacaccaggc
     3301 cttcaccgag ggcaagcaac cgaggctgcc caatctgtac cgtgccatca cgaccatgcg
     3361 gcgcgccatc aaagccgcca agagctacct ctgaggaacg atcgatggaa acgagcaacc
     3421 gcgtgctgga cgccgtactg aaggagctgt ccgtaccgca catcgaggag cagatcccgc
     3481 gtgacacgtt ctaccagctc gggctcaagt tccctcagca gtacggcatc gcttgctgcg
     3541 atgtcgccga ttgcgtgcgg cgagccgaag cgctcggcgc cgggcccttc attcacgcaa
     3601 cgctgtcccc acccaactgg gtcgagaacg gcgagccggt ccccgactgc aagctcgagg
     3661 tcgcactcgg ctatgcggga gacactcaga tcgagttcct aggacccggg gatggcaccg
     3721 agcactatgc gcgcgcgctc atcgagaccg acatcgcgtt ccatcatgtc gggatctacc
     3781 agcagggcat ggagcagctc gcggcatcga tcaccgctgc cggataccca gaagtggtcc
     3841 gcggtggaat ctcaatgggg a
//
LOCUS       DASRZF010000241         4519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2577707, whole genome shotgun sequence.
ACCESSION   DASRZF010000241 DASRZF010000000
VERSION     DASRZF010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2577707"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..1594
                     /locus_tag="VFG22_10585"
     CDS             98..1594
                     /locus_tag="VFG22_10585"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HET6416730.1"
                     /translation="MNARPRARRNLVLTAVFGLLIVVLVNYLSARHYKRWDWTSHGLF
                     TLSDRSRQVLHELDQDVRVHVFLGRDEQDFADVETLLEEYKALTDRITVEWVDPDRDR
                     ARYQALADKFGIDSWLAGDVTLSQVPVVVTSGERKWKVERHQLIVEDFETFEDADGHK
                     LDLQSERAITGAILEVTTGEPTRVCVSAGHGEWELGAYGDRSLDAVARELEWEKIEAE
                     AFEVRASDVPETCDALFVVSPQRPFSAEEVAIVDRFLAGGGNLLLAFDPVLKKEALTP
                     TGFEAWLEEHGVLLGKDLVIEHDPSQQLPGNPGDLFLVTNLGTHRLVDTIRKRGGGIL
                     LAVARSVKAVDGSNSEVLFRTSPDAVAETDIARALDPDAKGTPTARASAPLAAAWEYV
                     PPLDDDLKPVELEGPTPGRMLVVGDSDWMSPELLDNPQLSNIDFLSSSAGWLTQREAL
                     INIAPRKTNARAVIMSDADLQNLLFRVVVLLPLAAFIAGFGVWWSRRS"
     gene            1591..2199
                     /locus_tag="VFG22_10590"
     CDS             1591..2199
                     /locus_tag="VFG22_10590"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HET6416731.1"
                     /translation="MNTRTPIVLFIISAILLAYVLLFERGRPGRTEIDSRSGLLLETV
                     VRERITRIRIAAGEERIVLRREGEGFDETWTLEEPVPGAADPDAIEDYLRNWEFAIPV
                     RTLQSPSAEDIASFGIDKPKAEITFEMASAKTRVLLGSGTPVDGGGYVRIDEGPQVTV
                     IGEDVVALFTRTADSFMLRGDAGAPYLSDFVGPGIDAGDASP"
     gene            complement(2200..2802)
                     /locus_tag="VFG22_10595"
     CDS             complement(2200..2802)
                     /locus_tag="VFG22_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416732.1"
                     /translation="MGARLIGLILAGALLSAGCEQVTSASGSEAEAEQLPEIVPNLPE
                     VPTLPPPPFPTTYEDESYSVYGLRQRPTKLLDTDLSVTGYIIEIYEPPACTKRKKDEC
                     PKVAAPHMFIADTADQTERSEQLLVVGYADNQEQLARARRGRKTTTISGAVVVPKDLA
                     VGNKIVVKGHFRLMSSGGFNTSNGLIEYASHDTLAKSTAQ"
     gene            complement(2852..4303)
                     /locus_tag="VFG22_10600"
     CDS             complement(2852..4303)
                     /locus_tag="VFG22_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET6416733.1"
                     /translation="MGTTRRSRRALALALFLSATALPATALAGGDPTVIAEAVRRLDS
                     DHDRATAAIYVLQAGGAVAAEQIRDAWPALSLLGQARALRALAPLAERHGAALEALLE
                     AARSEDESLREPALAALGRITPRGRDGLVTLLPDPLVGDRAAEILASSEPRFALRRLL
                     DAMAEDGGSDRAGLRHALGIAVRLGGADADRDLRAWLEGRPDPEVVAAAALGLRGLEA
                     HRALLTSLIAYAAPRSTGFSTQWRLLQSAGLAGASQTVDRWLVEKLEHSPEWMLRHAA
                     VEAITARGHREEARGSLADPYPRVRARAAETLSGDPDSLLLRAALARRDVWPMVRAAA
                     VISLRSEGAAVPVIVASVDDSMSLVRAAAIDVLADASQPDGWDRIHRRLRDPNEWPNV
                     TAAAIEYVVAHCRTDAVDSLLRVVMRAAPSHALTEDLNNAALAIGALRALGSSESRSA
                     IELLQGTDGVPPTLKMALETPLAGDADCEPTRR"
BASE COUNT          823 a         1435 c         1451 g          810 t
ORIGIN      
        1 agggcgtggt ggatacccgc tacttggtgt tcgacctctc ggtggcggtc ttgacagttg
       61 gcctgagcat cggcgccttg aaggcacgga ggcacgggtg aacgcgcggc cacgagcacg
      121 tcgcaaccta gtgctaacgg cggtattcgg tctgctgatc gtcgtgctgg ttaactacct
      181 ctcggcgcgg cactacaagc gatgggactg gacgagccac ggattgttca cgctttcaga
      241 tcgaagtcgg caagtgctgc acgagctcga ccaggacgtt cgcgttcacg tcttcctcgg
      301 tcgcgacgag caagatttcg ccgacgtcga aacgctgctc gaagagtaca aagcgctgac
      361 tgaccgaatc accgtcgaat gggttgatcc tgacagggac cgcgcccgct atcaggcgct
      421 cgcagacaaa ttcggtatcg atagttggct cgcaggggat gtgacgcttt cgcaagtgcc
      481 tgtggtggtc accagtggcg agcggaagtg gaaagtcgag cgacaccagt tgatcgtgga
      541 ggacttcgag actttcgaag acgccgatgg ccacaagctg gatctacaat ccgagagggc
      601 gatcaccgga gccattctcg aagtcacgac cggcgagccg accagggttt gcgtgagcgc
      661 cggccacggc gagtgggagc ttggagcgta cggggatcgc agcctcgacg cagtggctcg
      721 tgagctcgaa tgggaaaaga tcgaggccga ggctttcgag gtccgtgcga gtgacgtgcc
      781 cgagacctgt gatgcgctct tcgtcgtgag cccgcagaga ccgttctccg cggaggaggt
      841 ggccatcgtc gaccgttttc tcgcgggcgg tggcaatctg ctcctcgcct tcgatcccgt
      901 gctgaagaag gaggcgctca cgccaacagg cttcgaagcc tggctcgagg aacacggagt
      961 cctcctaggg aaggatctgg tcatcgaaca cgacccttcg cagcagcttc cgggcaaccc
     1021 gggcgatctc tttctcgtga ccaatctggg cacgcaccgc ctggtggaca ccatacgcaa
     1081 gcggggcggc ggaattctct tggcggtcgc gcgctctgtg aaggcagtcg atgggagcaa
     1141 ctccgaggtc ctgttccgga cgagccccga cgcagtcgct gaaaccgata tcgcgcgcgc
     1201 cttggacccg gacgcgaaag gtacgccgac ggcacgcgcc agtgcgccac tcgcagccgc
     1261 ctgggagtac gtgccgcctc tcgacgacga cctgaagccg gtcgagctcg aaggccccac
     1321 accgggacgc atgctggtcg taggcgacag cgactggatg agccccgagt tgctcgacaa
     1381 ccctcagctg tcgaacatcg actttctctc ttcgtccgcc ggctggctca ctcaacgcga
     1441 agcgttgatc aacatcgcgc cgcgaaagac caacgcccgc gcggtcatca tgagtgatgc
     1501 ggatctgcaa aatctactct ttcgcgtcgt cgttctcttg cctctagccg ctttcattgc
     1561 cggattcggc gtatggtggt cgaggcgttc atgaacactc gaaccccgat tgtgctcttc
     1621 atcatctcgg cgatccttct tgcctatgtg cttctcttcg agcgcggccg ccccggacgc
     1681 accgagatcg actcgcgatc cgggctcttg ctggagacgg tcgttcgaga gcgaatcacc
     1741 cgcattcgaa tcgcagccgg ggaagagcga atcgttctgc ggcgcgaagg cgagggcttc
     1801 gacgaaacct ggaccctcga agagcctgtt ccaggggcag cggacccgga tgccatcgag
     1861 gattaccttc gcaactggga gttcgcaatc ccggtccgca cactgcagag cccgagcgcc
     1921 gaagacatcg cgagcttcgg gatcgacaaa ccgaaagcgg agatcacctt tgaaatggcg
     1981 tccgcgaaga cccgcgtctt gctcggttcc ggtactccgg tcgacggagg tgggtacgtt
     2041 cggatcgacg agggtcctca ggtgacggtc atcggagaag acgtcgttgc cctgttcacg
     2101 cgcaccgcgg acagcttcat gctccgcggt gatgctggcg ctccgtacct ttcggacttc
     2161 gtcggccctg gaatcgacgc cggcgacgct tcgccctgac tattgagcag tgctcttggc
     2221 gagtgtgtcg tgggacgcgt actcgatcag cccgttgctg gtgttgaatc cgcctgacga
     2281 catcaacctg aagtgtcctt tgacgacgat cttgtttccg accgcgaggt ccttgggaac
     2341 gacgacggct ccggagatgg tggtcgtctt tctcccgcgg cgcgcacggg cgagctgctc
     2401 ctgattgtcg gcgtacccaa ccaccaacag ctgctcggaa cgttccgtct ggtcggcggt
     2461 gtctgcgatg aacatgtgcg gcgcggcgac cttcggacac tcgtccttct tgcgcttcgt
     2521 gcaagcaggt ggctcgtaga tctcgatgat gtacccggtc accgaaaggt cggtgtcgag
     2581 cagcttggtc ggccgctggc gtaaaccgta tacgctgtac gactcatctt cataggtcgt
     2641 cggaaacgga ggcggcggca gtgtcggaac ctctggaagg ttcggcacga tttcaggaag
     2701 ttgctcagcc tcggcttcgc tccccgaagc ggaggtgact tgttcacaac cggcactcaa
     2761 cagcgcccca gccagaatca ggcctatcag acgagctccc atgtttcccc ctgtcctcaa
     2821 caaagtaaca gggcgattcg cgcgcgacaa tctagcggcg ggtgggctcg cagtcagcgt
     2881 ccccagccag gggcgtttcg agcgccattt tcagcgtggg cggaaccccg tcggtgcctt
     2941 gaaggagctc gattgcggag cgcgactccg agcttccaag agcccgaagg gccccaatgg
     3001 cgagggccgc gttgttgagg tcctcggtca gcgcatggct cggcgcagcc cgcatgacca
     3061 cccggagcag cgaatcgacc gcatcggtgc ggcaatgggc gaccacgtat tcgatcgcag
     3121 cggcagtgac gtttggccat tcgttgggat cgcgcagacg ccgatgaatg cgatcccatc
     3181 catcgggctg ggacgcatct gcgagtacgt cgatggcggc ggcgcgaacc agggacatcg
     3241 aatcatcgac ggaagcgacg atgacaggca cggccgcacc ctcgcttcgc agactgatca
     3301 cggcggcggc gcggaccatc ggccagacgt ccctgcgggc cagggccgcg cgcaggagca
     3361 ggctgtccgg atcgccggac aaggtctcgg cagcacgtgc acgcacacga ggatacggat
     3421 cggccaagct gcctcgcgct tcctcgcgat gaccacgggc cgtgatcgct tccacggcag
     3481 cgtgccggag catccattcg ggagaatgct cgagtttttc gaccagccat cgatcgacgg
     3541 tctggctcgc gccggccagt cccgcagatt gcagcagacg ccattgggtc gagaaccccg
     3601 tgctcctcgg cgcagcgtag gcgatcagtg acgtgaggag tgcccgatgc gcctcgagcc
     3661 cgcgcaggcc gagcgctgcg gcggccacca cttcgggatc gggtcgtccc tctagccagg
     3721 ctcgcaaatc gcgatcggcg tcagcgccac caagtcgaac cgcaatgccg agcgcgtgcc
     3781 ggagccccgc acggtcggag cctccgtcct cggccatcgc gtcgagcagg cgccgaagcg
     3841 caaagcgcgg ctcgctactt gccaaaatct cagcagcgcg atcgccgacg agcggatcgg
     3901 gcagcagcgt gaccagtcca tcgcgtcctc tgggggtaat acggccaagc gccgccaggg
     3961 caggctcccg caggctctcg tcctccgagc gagccgcctc cagcaatgcc tccagcgcgg
     4021 caccgtgtcg ctccgcgaga ggggcgaggg cgcggagcgc gcgcgcctgt cccaagaggc
     4081 tcagcgctgg ccaagcgtcc cgaatctgtt cggccgcgac tgcaccgcca gcctgaagca
     4141 cgtaaatcgc cgctgtcgcc cgatcgtgat cggagtcgag gcgtcgcacg gcttcggcaa
     4201 tcaccgtcgg atcgcccccc gctaacgcgg tcgcaggaag cgctgtcgcc gacaagaaga
     4261 gcgctagtgc gagagcgcgt ctgctgcgcc tggtcgttcc cactcgatta ccgttccgtc
     4321 tcgcagctct agcacgcagt ccgcgtgtcg aagcaaatcc tcgcgatgcg ttgccatgat
     4381 cacgatgtca ccacgatccg ctcgctcgcg gcggctgcgg ggctatcaag agcccgggga
     4441 tggctagtct ggtgtccacc gccagcgcca tagtggctag tgcagttacc acaagcgcgc
     4501 tccgaaggcc ctggacgaa
//
LOCUS       DASRZF010000242         4545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4302534, whole genome shotgun sequence.
ACCESSION   DASRZF010000242 DASRZF010000000
VERSION     DASRZF010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 4545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4545
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4302534"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..1825
                     /gene="lepA"
                     /locus_tag="VFG22_10605"
     CDS             29..1825
                     /gene="lepA"
                     /locus_tag="VFG22_10605"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HET6416734.1"
                     /translation="MSQDLQHIRNFCIIAHIDHGKSTLADRILEVTGAVSAREQKAQL
                     LDRMDLEQERGITIKAQGVRLPYKARNGETYQLNLIDTPGHVDFSYEVSRSLAACDGA
                     LLVVDATQGVEAQTLANVYLAVENDLEIIPVFNKIDLPSADVDRVRREVEDVVGLDCS
                     ELLPCSAKTGVGVPDILEAVVKRIPPPETDDSGFLRALVFDSWYDSYRGAVCMIRVKD
                     GTVRKGDKVRLMATGAEHEVTELGVYTPFQKELDHLGPGEVGYLAASIKSLQDAKVGD
                     TITQAKKPAQEALAGFKLIKPMVFCGIFPTDSDKYTELRDALEKLNLNDAAFMFEPET
                     SDALGFGFRCGFLGLLHMEIIQERLEREYDIGLITTAPSVIYRVTTEEATIEIHNPSQ
                     IPDGRIRIEEPYFTVSIHVPVEFVGAVIKLCQDRRGEQQGIQYASPERVIITYLMPLA
                     EVLFDFFDKLKSATKGYASMDYELAGYRENPLVRLDMLLNNQKVDALSAVVHKDKAYY
                     LGRALAAKLKKIVPRQQYEVIIQAAIGSKVIARETVRALRKDVTAKCYGGDISRKRKL
                     LEKQKEGKKRMKAVGSVEIPQDAFHAILKVDE"
     gene            complement(1838..2761)
                     /locus_tag="VFG22_10610"
     CDS             complement(1838..2761)
                     /locus_tag="VFG22_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416735.1"
                     /translation="MRCSLIRGLVLASALLTLASCNKTKTAADVDTGVRVLDQGDRPR
                     DRVRYKVPPGTTTSSTTTLRVRTLATSPDGAALSVLPGLRLDIVSGPAETTERGVKFK
                     VDVVNAEALLPAGLDPQTADMLRDGAKIVDQVGGWIEVDDRGQKLAGELDAGAKRSDV
                     PVRLLRMIANTRETLSRVLLPEEPIGLGARWETRRTINAYGIEIQQVNTYTLVDRVGD
                     ELMLNVTVQQRALPQSISFPEDGIEISVESISTLANGQIVLDLDALESDASANGSSED
                     RITVQTVEGKKPIRVDETFEIQVANTSGLVR"
     gene            complement(2771..3814)
                     /locus_tag="VFG22_10615"
     CDS             complement(2771..3814)
                     /locus_tag="VFG22_10615"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET6416736.1"
                     /translation="MTFTGAPRRAFALLLTAWLVASCAGDQTARVEKPSVEAEGVVDP
                     LPSWNDTPNKAKIIDFVRAVTDPDDPAFAEPEERVATFDNDGTLWVEQPMYAEMAFAL
                     HRVQDLSQVHPEWRTQEPFRAVLEGDKEALAASGRRGILEIVVASHTGMSTTLFESIA
                     TEWVRDALHPKFEQPYTELAYQPQLELLRYLEANGFKTFIVSGGSVDFMRTYTEATYG
                     IPPERVVGSSIETRYELQGGAPTLLRLPQVDFIDDKAGKPVGIHKQIGRRPILAFGNS
                     DGDFEMLEWTTTGGGGARLGLLLHHDDANREYAYDRESDVGRLGRGLDAAGSMGWVVV
                     SMKKDWKTVFAEN"
     gene            complement(3869..4159)
                     /locus_tag="VFG22_10620"
     CDS             complement(3869..4159)
                     /locus_tag="VFG22_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416737.1"
                     /translation="MIARQSEAHTRALAKRFDVPIKFCVDADGRLARRLNIEHLGGVP
                     FLMGALGYEADSVLPTVVIVDPSRKIVFADLTDNYRVRPEPSTFLAALDSKR"
     gene            complement(4156..>4545)
                     /locus_tag="VFG22_10625"
     CDS             complement(4156..>4545)
                     /locus_tag="VFG22_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416738.1"
                     /translation="MIRGPQVSLAEVGALLAIVPLVAFMSSLLMFKRARTSRNQYLML
                     ALSLIATGLAFVWFASPASWYALFLGVVPSFAYVFWYSVLDRPVPVVIAVGETLPELE
                     LVDEEGVRVTPSPRNSESSRHGVSRSS"
BASE COUNT          932 a         1405 c         1379 g          829 t
ORIGIN      
        1 ccgggtgcga ggctagagag gtcgcacttt gtcgcaagat ctccagcaca tccggaactt
       61 ctgtatcatc gcgcacatcg atcacggcaa aagcacgctg gccgatcgaa ttctcgaggt
      121 gacgggggcg gtttcggctc gcgagcagaa ggcccagctc ctcgatcgga tggacctcga
      181 acaggagcgc ggcatcacga tcaaggcgca gggcgtccgg cttccctaca aagcccgcaa
      241 cggcgaaacg tatcaactca acctcatcga tacgccaggc cacgtggact tcagctacga
      301 ggtttcccgc tcgcttgccg cctgtgacgg cgcccttctc gtggtcgatg caacccaagg
      361 cgtcgaagca cagacgctcg ccaacgtgta cctcgccgtc gagaacgacc tcgagatcat
      421 cccggttttc aacaagatcg acctcccgag cgcggatgtc gaccgggtcc gtcgagaggt
      481 tgaagacgtc gtcgggctcg actgttcgga gttgctccct tgcagcgcaa agacgggcgt
      541 tggggttccc gacattctcg aagccgtggt gaagcgaata ccgccacccg aaacggacga
      601 cagtggattt ctgcgcgccc tggtgttcga cagctggtac gacagctatc ggggcgcggt
      661 ctgtatgatc cgggtcaaag acggcacagt ccgcaagggc gacaaggtgc gtttgatggc
      721 cacgggcgca gagcacgagg tcactgagct cggcgtctac acgccgtttc agaaagagct
      781 cgaccacctc ggacccgggg aggtcgggta cctcgccgcc agcatcaagt cgctgcagga
      841 cgccaaggtg ggcgacacca tcacacaggc gaagaaaccg gctcaagaag ccctcgccgg
      901 cttcaagctg atcaagccca tggtgttctg cggaattttt ccaacggaca gcgacaagta
      961 caccgagctc cgcgacgcgc tcgagaaact caacttgaac gacgcggcgt tcatgttcga
     1021 gccggagacc tcggacgcgc ttggattcgg ctttcgctgt ggtttcctcg ggctgcttca
     1081 catggagatc atccaggagc ggctcgaacg agagtacgac atcggactga tcacgactgc
     1141 gccgagcgtg atctatcggg tgactaccga ggaagcaacg atcgagatcc acaacccatc
     1201 gcagattccc gacggtcgca ttcgcatcga ggagccctac ttcacggtct cgatccacgt
     1261 gcccgtggag ttcgtgggcg cggtgataaa gctgtgccaa gaccggcgcg gagagcagca
     1321 agggattcag tacgcatcgc cggaaagagt gatcatcacc tatttgatgc ctctggccga
     1381 agtgctcttc gatttcttcg acaagctcaa gagcgccacc aagggttacg catcgatgga
     1441 ctacgagctc gctgggtatc gtgagaaccc tctcgtccgt ctcgacatgc ttctcaacaa
     1501 tcagaaggtc gacgccctca gcgccgtcgt tcacaaggac aaggcctact acctcgggcg
     1561 cgcgctcgcg gcgaagctga agaagatcgt gccccgtcag cagtatgagg tgatcatcca
     1621 ggctgcgatc ggatccaaag tgatcgcccg ggagactgtc cgtgcactcc gcaaagacgt
     1681 caccgcgaag tgctacggcg gagacatctc gcgaaagcgc aagcttctcg agaagcaaaa
     1741 agaaggcaag aagcgaatga aggcggttgg gagcgtggaa atccctcagg acgccttcca
     1801 cgcaatcctc aaggtcgacg aataatctct gcgggcgcta gcggactagg ccggaggtgt
     1861 tggccacctg aatctcgaag gtctcatcca cacggatggg ttttttcccc tcgaccgtct
     1921 ggacggtgat gcggtcctcg gagcttccat tggccgatgc gtccgattcg agcgcatcga
     1981 ggtcgagaac gatctgaccg ttcgcgagag tgctgatcga ctccacggaa atctcgattc
     2041 catcttcggg gaacgaaatg gattgtggaa gagcccgttg ctgaacggtc acgttcaaca
     2101 tgagctcatc gcccacgcga tcgaccaggg tgtacgtatt cacctgctgg atctcgattc
     2161 cgtaagcgtt gattgttcgg cgcgtctccc agcgcgctcc gaggccgatc ggctcttcgg
     2221 gaagcagtac tcgcgagagc gtctcacgcg tattggcgat catccgaagc aagcgcaccg
     2281 gaacgtccga gcgcttagcc cctgcgtcga gctcgcctgc gagcttttgt ccacgatcgt
     2341 ctacctcgat ccagccgccc acttgatcga cgattttggc gccgtctcgg agcatgtctg
     2401 ccgtttgtgg atccaaccca gccggaagga gggcctcggc gttgacgacg tcgaccttga
     2461 acttcacgcc tcgctcggtc gtctctgccg gcccggaaac gatgtcgagt cgaagaccgg
     2521 gcaagaccga gagcgccgct ccatcggggc tggttgccag agtcctgacg cgcaacgtcg
     2581 tcgtcgagct ggtcgtcgtc cccgggggta ctttgtagcg aacccgatca cggggccgat
     2641 cgccttggtc gaggacgcga acgccggtgt cgacgtcggc tgcggtcttc gtcttgttgc
     2701 agctggcaag cgtcagaagc gccgatgcga ggacgagccc gcgaatcaat gagcatctca
     2761 tcatgaaccc ctagttctcc gcaaagacgg ttttccagtc tttcttcatc gaaaccacga
     2821 cccatcccat ggaaccggca gcatcgagcc cccggccgag tctccccacg tcgctttcgc
     2881 ggtcgtaggc gtattcgcga ttcgcgtcat cgtgatgaag caagagtccg agccgcgcgc
     2941 cgccgccgcc cgtcgtcgtc cactccagca tctcgaagtc gccgtccgaa ttgccgaatg
     3001 cgaggatcgg ccggcgaccg atctgcttgt gaatgcccac cggcttcccc gctttgtcat
     3061 cgatgaagtc aacctgcgga agccgcagga gcgtgggcgc cccaccctgg agctcgtacc
     3121 gagtctcgat ggacgaaccc acgacgcgct caggcgggat gccataggtc gcctcggtgt
     3181 aagtgcgcat gaagtcgacg ctccctccgg aaacgatgaa ggtcttgaaa ccgttcgctt
     3241 cgaggtagcg caagagctcg agctgcggtt gataggcgag ctccgtatac ggctgctcga
     3301 atttcggatg gagcgcgtct cgcacccatt cagtggcgat tgactcgaaa agcgtggtgc
     3361 tcatcccggt gtgggaagcg accacgattt cgaggatgcc gcgcctgcca gacgccgcaa
     3421 gagcctcttt gtcgccctcg agaaccgccc tgaacggctc ttgcgttcgc cattcggggt
     3481 gaacctgcga caggtcttgg acgcggtgaa gagcaaaggc catttccgca tacatcggtt
     3541 gctcgaccca gagcgtgccg tcgttgtcga aggtcgccac cctctcctcg ggttcggcaa
     3601 aggccggatc gtccgggtcg gtgaccgcgc gaacgaagtc gatgatcttc gccttgttcg
     3661 gagtgtcgtt ccaggaggga agcgggtcga cgacgccttc agcctcgacg ctcggcttct
     3721 ctacccgcgc cgtctggtcg ccggcacaac tcgcgacgag ccacgccgtc agaaggagag
     3781 cgaaagcccg ccgaggagct ccggtgaatg tcatggcgcg aactctaagc gtgggtccga
     3841 tgtccgtcca gggctcgtag ccgctttttc agcgcttcga atcgagcgcc gcgaggaagg
     3901 tgctcggctc gggccgaacg cgataattat ccgtcagatc ggcgaagacg attttgcggc
     3961 tgggatcgac gatcacgacc gtgggcagga cggagtcggc ctcgtaaccg agcgcaccca
     4021 tgagaaacgg cacgccaccg aggtgctcga tgttcaatcg ccgcgcgagt cgaccgtctg
     4081 catcgacaca gaacttgatc ggtacatcga agcgcttcgc cagagcgcgc gtgtgcgcct
     4141 ccgactgccg cgcgatcaag aagacctcga caccccgtgc ctcgagctct cgctattgcg
     4201 cggcgaaggc gtgacccgca ccccttcctc gtcgaccagc tcgagctccg gcaaggtttc
     4261 gccaaccgca atcaccactg ggaccgggcg gtcgagaacc gagtaccaga agacgtaggc
     4321 gaatgaggga actacgccga gaaacagcgc gtaccagctc gcaggtgacg cgaaccaaac
     4381 gaatgcgagc ccggttgcaa tcagcgaaag cgccagcatg agatactgat tgcgggacgt
     4441 gcgggctcgc ttgaacatca gcagagacga catgaaggcg acgagcggca caatcgccag
     4501 cagcgccccg acctcggcaa gcgagacctg cggtcctcga atcat
//
LOCUS       DASRZF010000243         3699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4983771, whole genome shotgun sequence.
ACCESSION   DASRZF010000243 DASRZF010000000
VERSION     DASRZF010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4983771"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VFG22_10630"
     CDS             <1..432
                     /locus_tag="VFG22_10630"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6416739.1"
                     /translation="FIVTTLVPAWRDPIALYGHALRIEPSSATAHYGLGSAVMGTAGC
                     EVGEPLFLRAVELDPSYLRAYTNLSVCEMRRGALDEAAATLERALELSGGLHPKVWAN
                     LAEVRLGQGNTLDACAHLERAIALDPGYAFAEELLAKHCQR"
     gene            782..1732
                     /locus_tag="VFG22_10635"
     CDS             782..1732
                     /locus_tag="VFG22_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416740.1"
                     /translation="MKREKIIGAAIVLIVVLGFLRCIDGLWIDGNNGWGGTGGFAGGV
                     GGFAGTGEDGGVGGVAGTGGTGGVGGSSGNGGTGGPSGFVCGDAICAEGEDLSNCAAD
                     CDHDLVVVVERALAGTLADSLNTYVEDLRVRGKRVRIEAWEPGTVQELKAVLFDQVDR
                     YGTEGALLVGNLPTAFYEHAVICIAWDRIDRCVMYEYEEFPTDVYLQDRNASWTDSDG
                     DGRYDSHDELDLEIFVSRLQTPPEKQKCEKSELFPECPENYEPGEAFYTREECVLKCP
                     SRLRMQIWQEYELGDVECCGPHFLRCYFERDHRYRSELSF"
     gene            2086..>3699
                     /locus_tag="VFG22_10640"
     CDS             2086..>3699
                     /locus_tag="VFG22_10640"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET6416741.1"
                     /translation="MPEARERAAALQEARRLYAEHLWSGAFEALSRLDEQEALAAADL
                     EQLAWSAALIGRDETFLKALERLYHTHLDAEAMSTAARFAFWLGFRLMFLGEAARGAA
                     WLGRCQQIVNECGEECAETGYLLLLPIRKQLMSGDLASASETAARAAAIGKRFSEPDL
                     VALSLHLGGRSLVQAGRVDEGMALFDESMLAATSGELSPIVTGIVYCAVIASCQQVFA
                     LERAREWTAALTQWCEAQPELMTFRGSCQVHRSEILQLAGDWEQALAQAERASVGDLA
                     AADRDAVADAFYMKAEIHRLRGELSQAEDAYLRSSQLGREPQPGLALLRAAEGRQRVA
                     LNALRRLLAETTERLARARLLPALFEISLDLGELTEAGAAATELESTASQYASEILGA
                     ISAHARGLLLLAQGDAQGALGPLRRAFYVWQRLGAPYEEARSRLALSKACQGLGDSEG
                     ARLSLDAARTLFEQLGAARDLAALDQPRAKSDSTHSLSPRELQVLLLVATGKTNRMIA
                     EHLQLSEKTIDRHVSNIFNKIDVASRSAAT"
BASE COUNT          713 a         1116 c         1200 g          670 t
ORIGIN      
        1 ttcatcgtga ccaccttggt gcccgcctgg cgcgacccca tcgcacttta cggacacgcg
       61 ctgcgaatcg aaccgagctc cgcaaccgca cactacggcc tcggttcggc ggtcatggga
      121 accgccggct gcgaagtcgg cgaaccgctg tttcttcggg cagtcgagct cgatccgagt
      181 tacctacgcg catacaccaa cctctcggtc tgtgagatgc ggcgcggcgc gctcgatgaa
      241 gccgctgcaa ccctcgagcg tgcgctggag ctcagcggcg ggctgcatcc caaagtctgg
      301 gccaacctcg ctgaagtcag actgggccag ggcaacaccc tcgatgcctg cgcacacctc
      361 gaacgcgcga tcgctctcga cccgggctac gcgttcgccg aggagctgct ggcgaagcat
      421 tgccagcgct gacaactatc gtaagagcgt cggctcctcg tctgaccggc ctgaatgcgc
      481 cgcacgttgc cggtgatggg cgcacgaccc ggcaaaagaa cgagcgcaca gattgcgcga
      541 gccccgaggt tcggcgaaaa gctgagcact tgcgtgtcgg tcatgaggtg agcgcgggtg
      601 caacatctgc gctcacgaag gcagcaacaa atacgtatag aaacatcgac ccaggggatc
      661 cagattctct gcgataagac gccgacaaag tgcggtgtca attcggggtt cccccgatcg
      721 gccgccggcc agcctggcga cgatcgcgag tccaattgca aaccgggaat ggatgagcgt
      781 gatgaaacgc gaaaagatca taggtgcagc cattgtgctg atcgtcgtgc tcggcttttt
      841 gcggtgcatc gatgggctct ggatcgacgg caataacggt tggggaggca caggcggctt
      901 cgcgggtggt gtcggagggt ttgccggaac aggagaggac ggcggtgtcg gaggggtcgc
      961 ggggaccggc ggaaccggcg gagttggagg gagctcgggg aacggtggaa ccggcggacc
     1021 gtcaggtttc gtttgtggcg acgcgatctg cgccgaaggc gaggacctct cgaattgcgc
     1081 agccgactgc gaccacgacc tggtcgtcgt ggtagagaga gccctggctg gaactttggc
     1141 cgatagcctc aacacgtacg ttgaagacct gcgagtgagg ggcaaacggg ttcgcatcga
     1201 ggcctgggaa cctggcacgg tccaggaact gaaagccgtc ctgttcgatc aagtcgatcg
     1261 atacggtacc gaaggcgcgc tcctcgttgg caatctacct accgccttct atgagcacgc
     1321 ggtcatctgc atagcttggg acaggatcga tcgttgcgtg atgtacgagt acgaagagtt
     1381 cccaaccgac gtgtatctcc aggatcgaaa cgcaagttgg accgactccg acggggatgg
     1441 aagatacgac tcgcacgacg agctagattt ggagatcttc gtctcacgct tgcagacgcc
     1501 tccagaaaag caaaagtgtg agaagagcga gctctttcca gagtgccccg agaactacga
     1561 acccggcgaa gcgttctaca cgcgcgaaga gtgcgtcctg aagtgccctt cgcgattgcg
     1621 aatgcagatt tggcaggagt acgagcttgg agacgtcgag tgttgcggcc ctcacttctt
     1681 gcgttgctat ttcgaacgcg accatcggta tcgcagcgag ctgtcgtttt gaagacccgt
     1741 gaaggccaag ccgaacgtcc tcagcgtaac caaatgtcgc gtgtacctca tgacgcacct
     1801 cctagcggcg ggcatccagc caggggaccg agggtcggga ctgagcccgc attggtactg
     1861 tgcgcatccg aagaggcacc gaaaatacgt aattgttgcg agcacgtacg taaatgttca
     1921 ctcgatcccg gaagtcagga tgagcgatca aactttcgca gcgaactttt gcgagagagt
     1981 ggtcattccg atgttgctct atggatgatg cgacgttttg cggcgtctac atgtcggtgc
     2041 tcattcggcc ttgtcaaagg agctcgcgat ggggatgctc ggcccatgcc cgaggcaaga
     2101 gagcgagcgg cggctttgca agaggcccgc aggctctacg cggagcacct ctggagtggt
     2161 gccttcgagg ctctgagtcg cctcgacgaa caagaagcgt tggccgctgc cgatctggaa
     2221 caacttgctt ggtcggcggc gctgatcggg cgagacgaga cgtttctgaa agctctcgag
     2281 cgcctctatc acacgcacct cgatgcggag gccatgtcga ccgccgcccg cttcgcgttc
     2341 tggctcgggt ttcgactgat gttcctcggc gaggcagcgc ggggagctgc ctggctgggt
     2401 cggtgccagc agatcgtgaa cgagtgcggg gaggagtgcg cggagacagg ctacttgctc
     2461 ctattgccaa ttcgcaagca gctcatgtcc ggcgaccttg cctccgcctc ggagacggcc
     2521 gcgcgcgcgg cagcgatcgg taaacgcttc tccgagccag atctggtcgc gcttagcctt
     2581 cacctcgggg gacggtccct cgtccaggcc ggtcgcgtcg acgagggcat ggcgctcttt
     2641 gatgagagca tgcttgcggc tacgtccggt gagctgtcgc cgatcgtgac cgggatcgtc
     2701 tactgcgccg tcatcgcgtc gtgtcagcag gtgttcgcgc tcgaaagagc gcgcgagtgg
     2761 actgcggccc tcacgcaatg gtgtgaagcc caacccgagc tcatgacctt tcgcggcagc
     2821 tgccaggtgc atcgctccga gattcttcag ctcgccggcg attgggaaca agcgcttgcc
     2881 caagcggaac gagcgagcgt cggagatcta gcagctgccg accgcgatgc cgtcgccgac
     2941 gcgttctaca tgaaggctga gatccaccgc ctacgcggtg agctctcgca agccgaggat
     3001 gcctacctgc ggtcgagcca acttgggcgc gaaccacagc ccggtctcgc cctcttgcga
     3061 gccgccgagg gacgtcagag agttgcgctc aatgcgctgc ggcgtcttct cgctgagacg
     3121 accgagcggc tcgctcgcgc gcgactcctt ccggcgctct tcgagatcag tctcgatctg
     3181 ggagagctga cggaggccgg tgccgccgcc accgagctag agagcaccgc ctcgcagtat
     3241 gcgagcgaga tcctcggagc gatttccgcg cacgcccgag ggctattgct cctcgcgcaa
     3301 ggcgatgccc aaggggcgct cggaccactt cgtcgcgcct tctatgtctg gcaacggctc
     3361 ggtgcaccgt acgaagaagc ccgatcgcgg ctcgcactga gcaaagcatg ccagggtctc
     3421 ggcgattcgg agggtgcgcg cctctcgctc gatgcggcgc ggacgctctt cgagcagctc
     3481 ggcgcggcgc gtgacttggc ggcgctagat caaccgcgcg ccaagagcga ctcgacccac
     3541 agcctcagcc cccgcgagct ccaggtgctc ctgctggtgg cgacgggcaa gaccaaccgg
     3601 atgatcgcgg agcatctgca actgagcgag aagacgatcg atcgccacgt cagcaacatt
     3661 ttcaacaaga tcgacgtcgc ctcgcgctcg gccgccacc
//
LOCUS       DASRZF010000244         2989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1897201, whole genome shotgun sequence.
ACCESSION   DASRZF010000244 DASRZF010000000
VERSION     DASRZF010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1897201"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VFG22_10645"
     CDS             <1..207
                     /locus_tag="VFG22_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HET6416742.1"
                     /translation="VHEHWPYGGPGAEIVDRIQREAFDYLDAPVERVTGLDVPMPYAL
                     NLEDKVLPTEERVIDAVKRVSYVK"
     gene            211..1473
                     /locus_tag="VFG22_10650"
     CDS             211..1473
                     /locus_tag="VFG22_10650"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex dihydrolipoamide
                     acetyltransferase"
                     /protein_id="HET6416743.1"
                     /translation="MAKIIGLPKLSPTMEEGTLVAWIKKEGDEVEVDDLLAEVETDKA
                     TMEFRSFDRGVLLKILVKEGETLEPDVPVAIIGKAGEDIGELLAQVEAGAAAASEPVS
                     EPAAVSEPAAVSAAAPVSAPAAVSEPVPVVDTESSDRILSSPLVRRLAREKSIDLRAV
                     EGSGPRGRIIKRDIDSYEERPSSARTYSGERLPPRVLQASSMRRTIARRLTESTQTIP
                     HYYLTIDVDIAPLADAREAMNAELAQDGRKVSLNDLIIKSAAVALRRVPEVNASWMGK
                     EIHYHQVVDISIAVSVEDGLMTPVIRDADQKGIAEISDEVRELAARARDKKLQPEEMS
                     NGTFSISNLGMFGIEEFTAVINPPEAAILAVGTIRHEPVVTEQGIVPGRRMRFTMSCD
                     HRIIDGATGAKYMAALKRILEAPANMLL"
     gene            1599..1886
                     /locus_tag="VFG22_10655"
     CDS             1599..1886
                     /locus_tag="VFG22_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416744.1"
                     /translation="MTSRGTILIGLLLCTALLAVTRLAPAPGDGGECKAIEIETNEAG
                     YKSGYHLFAERDVVYTWFTGEAGLVVESDSGKRTEFAPQSIDSFKCMLEPP"
     gene            complement(2251..2571)
                     /locus_tag="VFG22_10660"
     CDS             complement(2251..2571)
                     /locus_tag="VFG22_10660"
                     /inference="COORDINATES: protein motif:HMM:NF044456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HET6416745.1"
                     /translation="MLGRPKDALIRWAQLGAFSPVMENGGGGEHRPWMFDEETTDIFV
                     AAILEEGTSRTLTFPEGRWVYLFDSSKTFDGETEVTLEIPISEFPAFVREGSDIAATL
                     LADR"
BASE COUNT          559 a          908 c          982 g          540 t
ORIGIN      
        1 gttcacgagc actggcccta cggcggcccg ggtgccgaga tcgtcgaccg gattcaacgg
       61 gaggcgttcg actatctcga cgcgcccgta gagcgggtaa cggggctcga cgtgccgatg
      121 ccctacgccc tgaacctgga agataaagtc ctgccaaccg aggagcgagt gatcgacgcg
      181 gtcaaacgcg tcagttacgt caagtgagtc atggctaaga tcatcggact acccaagctt
      241 tctcccacca tggaagaagg caccctcgtc gcctggatca agaaggaggg cgatgaggtg
      301 gaggtcgacg accttctcgc ggaggtcgaa accgacaagg ccacgatgga gttccgctcc
      361 ttcgaccgcg gcgtcttgct caaaatcctc gtgaaggaag gcgagaccct ggagccggat
      421 gtacccgtgg cgatcatcgg gaaggccggc gaggacatcg gggagctgct ggcgcaggtg
      481 gaggccgggg cagcggcagc gtcagagcca gtgtcggagc cagcggcagt gtcagagcca
      541 gcggcagtgt cggcggcagc gccagtgtca gcgccggcgg cagtgtcgga gccagtgcca
      601 gtggtcgaca cggagtcttc cgatcgaatt ctctcttcgc ctctcgttcg tcggcttgcc
      661 cgcgaaaaga gcatcgatct tcgtgcggtc gaagggtcgg ggccgcgggg ccggatcatc
      721 aaacgggata tcgactccta cgaagaaagg ccgagctccg cacgaacgta tagtggcgag
      781 cgtctcccgc cgcgcgtgct gcaagccagc agcatgcggc ggacgattgc gcggcggctc
      841 accgaatcga cgcagacgat tccgcactac tacctcacca tcgacgtcga tatcgcgccg
      901 ctcgcagatg cccgcgaggc gatgaacgcc gagcttgccc aggatggtag gaaagtttct
      961 ctcaacgatc tgatcatcaa gtccgccgcg gtggcgttgc gaagggtgcc cgaggtgaat
     1021 gcctcctgga tgggcaaaga gattcactat catcaggtcg tggacatctc gattgccgtg
     1081 tccgtcgagg atggcttgat gacaccggtg attcgcgatg ccgaccaaaa gggaatcgcc
     1141 gagatctccg atgaggtccg cgagcttgcc gcgcgcgccc gcgacaagaa gctccaaccc
     1201 gaggagatga gcaacgggac gttttcgatc agcaacctcg gcatgtttgg aatcgaggag
     1261 ttcacggctg tcatcaatcc gcccgaggca gcgatcctcg cggtgggcac gattcgccac
     1321 gaaccggtgg taacggaaca gggcatcgtt cccggtcgcc gcatgcgatt cacgatgagc
     1381 tgtgaccacc gcatcatcga cggggccacg ggcgcgaagt acatggcggc gctcaagcgg
     1441 attctcgaag cgccagcgaa catgctgctc taggcgaggt gccgggtcgg gttcccccgc
     1501 gcccctccct gcgtcgacat cactgtcagc ctccgtgcca gcgtcgcgcc gagatcttcg
     1561 tttctgagcg gcatccctgt tagggtggtc ggtgcacgat gacttctcgg ggtacaattc
     1621 tgatcggtct gctcctttgc acggcgctct tggccgtaac gcgcctcgcc cctgcaccgg
     1681 gtgatggcgg ggagtgcaag gcgattgaga tcgagacgaa cgaggccggg tacaagtcgg
     1741 ggtatcacct gttcgcggag cgtgacgtcg tctacacctg gttcaccggt gaggcgggct
     1801 tggtagtcga aagcgacagt ggcaagcgta cggagttcgc tccgcagagc atcgacagct
     1861 tcaagtgcat gctagaacca ccctagattg gcgcgtggct gggtcaggct tgcgcccact
     1921 gcgcatgctt cgccgatagt cggtcgccac tggcaatcgg ttcaccgcag ccgcacccca
     1981 tcgttggcgc tggcgctgtc cctgacgctg gcactgacgc tagcgctggc accgacaccg
     2041 tggctcacgt ggatgctcgc gcgtacgtgg acgccgacgg aggagggcgg ctcgggatgg
     2101 gcagcacagc gattgggtcg agcctagccg tacgtcgtct tagagaactg ggggcgagtg
     2161 ggtcccaatc gcgagcacgc cggtcgcgag ccgtcctccg gtgcgcagcc gcgcaacaca
     2221 attgctgaca atggccggcg ccccgcttgc ctagcggtcc gctaggaggg tagccgcgat
     2281 gtccgagccc tctcgaacga atgccgggaa ctccgagatc gggatctcca gcgtgacttc
     2341 cgtctcgcca tcgaacgtct tgctcgagtc gaacagatag acccagcgac cctcggggaa
     2401 cgtgagcgtt cgcgatgttc cttcttcgag gatcgcagcg acgaagatgt cggtggtttc
     2461 ttcgtcgaac atccaggggc gatgctcgcc gccgccgccg ttctccatga ccgggctgaa
     2521 ggcgccgagc tgggcccagc ggattaacgc gtccttgggt cgaccgagca ccgtcttggt
     2581 cgccgaccca gccagcccag ttcacgtcga tcggcgcata gacccaaatg tcggcgtcga
     2641 tctcggtgcc caggttgtcg acgggacgcg ccgtgatgat tcggtcgtcg ccgagctttt
     2701 ggcgcagata gtcgaacgag tcacggtagt acgcatagcc gtattcagcc ccgagcgcga
     2761 tcgctttgtc catctgctcg tgccaccact gcactgcgta ggggttgaag tagtcgatca
     2821 gcgcgatcgc gctttactgg gtgctctcgt tctctaaaac ccagtggtgg aacgcccact
     2881 cgggccaagg tgcgacggga tctacgcttg cgtctgggcc ggcatccgca cccccgtcgg
     2941 gcgtgatgct cgttgacgac gagcaggcta cgagggtaga aagtacgaa
//
LOCUS       DASRZF010000245        12272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_520066, whole genome shotgun sequence.
ACCESSION   DASRZF010000245 DASRZF010000000
VERSION     DASRZF010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 12272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12272
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520066"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="VFG22_10665"
     CDS             complement(<1..752)
                     /locus_tag="VFG22_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416746.1"
                     /translation="MRSGIIVLAVATLAWMQAVPVGAYTIQSGLTEGCHERITAEAFV
                     AFLDDPAWAEVVVPEGSTWRSLAAPLNRWLLDEGLIQEDLSEPQLFVLFSLVVGVRDP
                     DTNGRSSSDLATQRSIHGNPRPEAQYVHALRAPEDDEPDGSQAALTGTRALIRQSFAD
                     AVVVWREPSEAQISTAPITLDFYDLFGVEVWQPGFLLGKTAHALQDSFAHTIRSDATA
                     LRTIVHVLNYVDAIYKDFNESRDGIAHSRHLD"
     gene            complement(752..1327)
                     /locus_tag="VFG22_10670"
     CDS             complement(752..1327)
                     /locus_tag="VFG22_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416747.1"
                     /translation="MPLLFARAEPVEQPAVEPPPPAESVPEAKHVLPNEAFVRLELHF
                     SLLTELADRSDLVPAFGYGLKGGYRWGFVGLFLQFEQDFWVGTEYEVRVTQGAYNIAV
                     GVDFTYFEGRAHTSIAVGPSILAFNTPLDSRGSVGWFLDFRPVGFQWDVHRFVRLGLD
                     PISFAVVAPSLDGVPLVQIEYRTTFYLETAF"
     gene            complement(1369..2136)
                     /locus_tag="VFG22_10675"
     CDS             complement(1369..2136)
                     /locus_tag="VFG22_10675"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HET6416748.1"
                     /translation="MNHASLGTIVLGVVLAIPALTSFCVAIGFAQESIAKKRNRLIFD
                     VPLGKGQLRHEMIGTLTFHLIFVPCAAVVWWKLARFTSGWRADVATFLVCLYSFQIYY
                     YGFHRAMHTDALYRFHKWHHRSHVMTPLAGLSMGPVEALGWTLGILGPVVILGQVGWL
                     GAWGLLALLAFHWYGNITGHANADFIPRPFGTKLVSTFANPVTYHALHHARFQGHYGF
                     GAAYMDRLGRTEFSDWQKVHARVMTGRPLRTMGETVE"
     gene            complement(2227..4740)
                     /locus_tag="VFG22_10680"
     CDS             complement(2227..4740)
                     /locus_tag="VFG22_10680"
                     /inference="COORDINATES: protein motif:HMM:NF023454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3516 domain-containing protein"
                     /protein_id="HET6416749.1"
                     /translation="MSTLDRYLDDLGEKVDGDTLLTRFLEYVSDQGLELYPAQEEAVM
                     ELFVGNNVILNTPTGSGKSLVALAAHFRSIALGERSFYTAPIKALVSEKFFDLCRVLG
                     PQRVGMMTGDATVNRDAPVICCTAEILANMALREGERAEVDGVVMDEFHYYADRDRGV
                     AWQIPLLTLPQARFLLMSATLGETQKFEDALTELTGVTTTLVKSTDRPVPLDFSYSED
                     TLQATVVELLEAEKAPIYIVHFAQRAATEQAQSFMSIDFLSKDEKRDLKEELSGTRFD
                     TPFGKELKKFLHHGIGVHHAGMLPRYRRLVERLAQEGKLRIICGTDTLGVGVNVPIRT
                     VLFTKLCKYDGVKTKVLTVRDFKQIAGRAGRRGFDTLGSVVAQAPEHAIENKKLRGKA
                     GGDAKKLRKLKMRQPPERGYAHWDEETFTRLQESPPEPLRSRFHVSNAMILNLFERED
                     NGCLALRKLIRSSHEGPTAKRNHARQAIAMIRSLRDAGVISLDADGPRVNEDFQSDFS
                     LNQALSLYVVEAVDVLEPEDPAYPLDVLTLVEATLEDPHAVLYKQTDTLKSRAIAAMK
                     AEGMEYDERMAELDKIEYPKPNAEFLYATYEVFSERHPWVGGDVLRPKSIARDMYELG
                     MSFVEYVKEYGLQRSEGVLLRYLTDAYKGFVQTVPESAKTDELYDVSDWLGLTVRSVD
                     ASLLDEWEQLQAPDEAALETAQHEQDQAVDITKDQRGFTTMVRNAVWKIVRFIAFKQY
                     ERAAEALSEASESNEWTAERFAEALAPYWSEYDAIQIGPDARSSAQVQIERSAADWKL
                     TQILLDPDEHRSWHLKFRVDLAASREAAAPQLTLETLSI"
     gene            5124..6167
                     /locus_tag="VFG22_10685"
     CDS             5124..6167
                     /locus_tag="VFG22_10685"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HET6416750.1"
                     /translation="MDPSLAVGDPSGKARINSGLRVSGAWTRGVVHSFRRLGLDVDAL
                     CKEIGVGVEVFMDTDGRPPRDASGRLWRAALAASGDRHLGLSAADAWEARADHLVVLL
                     LTSATSLGQGLEAGLRYQELLSHGRVITLGTHPELHVLHINKIEHELPITVHEVEFIT
                     VVVMKMLRFATDGAFKAEEIHFEHPYRGNIQKYTRAFGCSVLFGQEKTAIFADDASWT
                     LPLAHGNPALHGQLKSVAAGLHATLESHHFIDTVRERIKILLPRGQTSIEAVASALHM
                     TPRTLQRRLQEEDTTFRVLLDAVRKSILVECFERNQAPREIMRHAGYTNAKSFRRALK
                     RWNFSNAEDGLRS"
     gene            complement(6169..6804)
                     /locus_tag="VFG22_10690"
     CDS             complement(6169..6804)
                     /locus_tag="VFG22_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416751.1"
                     /translation="MRAQAKKPRISFAWLPLAVLSLGLTAQAQIPKTPDSSYVAPADQ
                     ALLIFDRPRHRQASETGIRVVTPAGKCIALLENDWQMAAAIWPGKQMLMLVTGMSPPV
                     VQLLEVNLSAGKTYVVRLRPRVNVKSPVEIEVLRREDQPLEAFPAAVREQLPAQPNLR
                     QCTEWVSWKRSKIGSRAGEAKRSWDEADKEFRDRNTVHRNDGWTANEVREP"
     gene            6936..8768
                     /locus_tag="VFG22_10695"
     CDS             6936..8768
                     /locus_tag="VFG22_10695"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6416752.1"
                     /translation="MRTSGVGSQAPALPATPGNQIPQIGDVLDSRYRITGVLGSGGMG
                     CVYLAEHVSIQRPLALKLLHPEVGDSEEVNKRFEREAFAIGRVDHPNCVDVSDFGKLQ
                     DGTHYMVLELLDGVLLSDLLERKQRLGWKRALHIGRHVLSALDYAHDAGIIHRDIKPE
                     NVILVEQDGDPDFAKILDFGIAKFRDDAKPEVQTGPLGNDAKLTQHGVAIGTPTYISP
                     EQAYGQSIDGRADLYSLSVMLYEMIAGVPPFDADEVGTLLRMHVSAEVPRFGDIAPEV
                     DVPAEVEALIRDGLAKKADERIATAHAYIARIDALVPLASGTGSGLRELPSIRVAAVR
                     DTTATAGVRALRRKWPSKWIAAAVLFLVAGTGLAVLGFRSDGPNYLPDGNILPLSSPA
                     HGPEAEVAAEMLAQGRAEEAASYLMEHRADLQEEPYAQMVLGHAQASAQRSIQALNAY
                     AKAIQLEPKLAKDKLMRTNIELMLAKKAPGIVDTALELLGTLVSEADDATAADQLIDL
                     ASSSKVTRRRHKAMSVADSVGLSDRVDRLGSYLLDLQLGESCPVRKSAVANLRALGDK
                     KAIPELRKARKRIRTEGGLIKRKVNTNACLRADAAEAIRYLQSL"
     gene            8926..9663
                     /locus_tag="VFG22_10700"
     CDS             8926..9663
                     /locus_tag="VFG22_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416753.1"
                     /translation="MKSSASILTILASVMVLQSCGSDTSQGSTELAAVLDAAADREGV
                     APGDCRDACLAAALEVFDACAAESDDREACFGQAIHAFFECAKTCEPPSCEARCENAA
                     SEMYEACVAGGGTEDECAMAARESLAACKAEHCEAGLTCEDECGMLAEQVYDVCMEKL
                     DSEDRCAEIAGGAARLCKKHCEGYQCPCHDDDCDDEDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDKDKDCHEGHPDWEKSSGKCK"
     gene            9731..10504
                     /locus_tag="VFG22_10705"
     CDS             9731..10504
                     /locus_tag="VFG22_10705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HET6416754.1"
                     /translation="MRVLSFNANGIRAAARKGFYDWLPEQQADFVCLQEIKARPEQLS
                     TAELEPAGYQCFYEPGERKGHSGVAILSREEPDEVIYGFGSREFDPEGRYIEARFGNL
                     SIVSVYVPSGTSNETRLAAKFRFMKQFSKHLRRLRKSGRQYVLSGDWNVAHKEIDLKN
                     YKSNQKNSGFLPEERAWLDQVFGRIGFVDAFREVNPEPEQYTWWSNRHPTTRDRNIGW
                     RLDYQVVTPELGARAVSASIAVEPRFSDHAPLLIEYRES"
     gene            10533..>12272
                     /locus_tag="VFG22_10710"
     CDS             10533..>12272
                     /locus_tag="VFG22_10710"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HET6416755.1"
                     /translation="MGAYLYPISLAGISVFVFALERFFPWRPEQKQFRPKLWSDFIHL
                     VFNGHFLGLLLAGLASTWVLPSVDRWLSAEGLTDAVYRNAAADWPLWAQIIVALLVLD
                     FLQWCVHNLLHRVPFLWELHKAHHSVKDGEMDWIVSFRFSWLEVIVYKSVLYLPLVFF
                     GFRWEALMFHAIFGTLIGHLNHSNLNLGHGPWRYVLNSPRMHIWHHDYDGTAKTTVNY
                     GIIFSCWDWIFGTAKMPADPPAHLGFGGVETFPDNFFGQEIWPLPKLLPRVHSTVVWS
                     IIGALVLAAAWYLREPRRLGPETPMLGEWAAASQPSGVFPAFDSYSPSPAEAEAEIAR
                     FGEQARADGYADPQLIVSVAELAKALGAPTLVLLDVRPEARFAVGHIPGARRLDREDY
                     SSGGEIPGLSRPAPELEQRLRALGVHRDSTVVLYGDGGPEPYRLWWTLRVTTALETRL
                     LDGGLQAWKSAGHRVAGGEGFEVAPGDVQLGAPASAPIQRWAETRAFMAEHPGVRLLD
                     TRSAVEFDGSEKHPKASRAGRIPGATHLDWVEVFRDEDEDHRLRLPAELRALLEARGV
                     AEGTPVVTYCQSGT"
BASE COUNT         2232 a         3912 c         3857 g         2271 t
ORIGIN      
        1 ttgtcgagat gacgcgagtg agcaatcccg tcgcgcgact cgttgaagtc tttgtagatg
       61 gcgtcgacgt agttgagaac gtgcacgatc gttctcaggg cagtggcatc ggagcgaatc
      121 gtgtgagcga agctgtcttg gagcgcatgg gcggtcttgc cgagcaggaa cccaggctgc
      181 cagacttcca ctccaaacaa gtcgtagaaa tcgagtgtga tgggtgctgt ggaaatttgg
      241 gcctcgctgg gctcccgcca cactacgacc gcatcggcga aggactggcg gatcaacgcc
      301 cgcgtgcccg tcagcgcggc ttggcttcca tccggctcat cgtcctcggg cgcccggaga
      361 gcatgcacgt actgcgcctc ggggcgggga ttgccgtgga tgcttcgctg cgtggctagg
      421 tcgctcgacg aacggccgtt cgtgtctgga tcgcgcacgc cgacgaccag gctgaagagc
      481 acgaagagct gaggctccga aagatcttct tggatcagcc cctcgtccag caaccatcga
      541 ttgagcggag ccgcaagact tcgccaggtg ctcccttcgg gaacgacgac ctcggcccag
      601 gccgggtcat cgagaaacgc cacgaacgcc tcggcggtga ttctctcgtg acatccctcg
      661 gtcaggcccg actggatcgt ataggcgcca actggcacgg cttgcatcca ggcaagcgtt
      721 gcgaccgcca agactatgat gccggagcgc atcagaacgc ggtctccaga tagaaggtcg
      781 ttcgatactc gatctgcacg agcggaacgc cgtcgagcga gggggcgacc acggcaaagc
      841 tgatcgggtc gagtcccagg cgaacgaatc gatgcacgtc ccattgaaaa ccgacgggcc
      901 ggaagtccag gaaccagccc acgctgcccc ggctgtccaa gggcgtattg aacgcgagga
      961 tcgaaggccc gaccgcgatg ctcgtgtgcg cgcgtccttc gaaataggtg aagtcgacac
     1021 ccaccgcgat gttgtacgcg ccctgcgtga ctcgaacctc gtattcggtt ccgacccaga
     1081 agtcctgctc gaactgaaga aacaagccca cgaaccccca gcggtaaccg cccttgagtc
     1141 cataaccgaa agccggcacg agatcggagc ggtcggcgag ctccgtgaga agcgagaagt
     1201 gaagctccaa gcgaacgaac gcctcattgg gcaagacgtg tttggcttcg ggcacgctct
     1261 cggctggcgg cgggggctca accgcgggct gttccacggg ctctgccctg gcgaagagca
     1321 atggcatgct tgcaagtccg aaaccgcaga gaaccaagaa gcgccccatc attcgacggt
     1381 ttcacccatt gtgcgaagtg ggcgacccgt catcacgcgc gcgtgcacct tctgccagtc
     1441 tgagaactcg gtgcggccca gtcggtccat gtaagccgct ccgaagccgt agtggccctg
     1501 aaagcgggca tggtgcagcg cgtggtaagt cacggggttc gcaaacgtgc tcaccagttt
     1561 cgtaccgaac ggcctcggaa tgaagtctgc attcgcatgg ccggtgatgt tgccgtacca
     1621 atggaaggcc agaagtgcga gcaagcccca ggcgccgagc cagccgacct gtcctaggat
     1681 gaccaccggg ccgaggatcc cgagcgtcca accgagcgcc tcgaccggac ccatcgagag
     1741 accggcaagt ggagtcatca catgagaccg gtggtgccac ttgtgaaaac gatagagcgc
     1801 gtcggtatgc atcgcccgat gaaagccata gtagtagatt tgaaacgagt ataggcagac
     1861 caggaacgtg gcgacgtcag cacgccaccc gctcgtaaac cttgcgagct tccaccacac
     1921 gaccgcagca cacgggacga aaatcaggtg aaaggtcagg gttccaatca tctcgtgccg
     1981 cagctgtccc ttgcccaatg gaacgtcgaa gatcaggcga ttgcgcttct ttgcgatgga
     2041 ctcctgcgca aagccaatcg cgacgcagaa gctggtcagc gccggaatcg cgagaacgac
     2101 gccgaggaca atggtgccta agctcgcatg attcatccag gctgccatct gcttcgagaa
     2161 tagattagca cgcctcgcgt cgctagaacg cagcagaggg catcatccat tcgatgcatc
     2221 gccgactcag atgctcaacg tctccagcgt gagctggggt gccgcggcct cgcggctggc
     2281 cgctaggtcc actcgaaatt tcaggtgcca gcttcggtgc tcgtcggggt cgaggaggat
     2341 ctgcgtgagc ttccagtccg ccgcgcttcg ttcgatttga acctgggcgc tgctccgcgc
     2401 gtcggggccg atctgaatcg cgtcgtactc ggaccagtag ggcgcgagcg cctctgcgaa
     2461 acgctcggca gtccactcgt tgctctcact ggcttcggag agcgcttcgg ctgcgcgctc
     2521 gtattgcttg aacgcgatga agcgcacgat cttccacacc gcgttgcgca ccatggtggt
     2581 gaagccgcgc tggtccttgg tgatgtcgac ggcttgatcc tgttcgtgct gggcggtctc
     2641 gagggcggcc tcgtcgggcg cttgcagttg ctcccattca tcgagcaggc tcgcgtcgac
     2701 ggagcggacc gtgaggccga gccagtcgga gacgtcgtag agctcgtcgg tcttggccga
     2761 ctcgggcacg gtttgaacga agcctttgta cgcatcggtc aagtagcgaa gcagcacgcc
     2821 ctcggagcgc tgcaatccgt actccttcac gtactcgacg aagctcatgc cgagctcgta
     2881 catgtcgcgc gcaatcgatt tcggacggag cacgtcgccg ccaacccatg gatgccgctc
     2941 ggagaagacc tcgtaggtgg cgtagagaaa ctccgcgttc ggcttcgggt actcgatctt
     3001 gtcgagctcg gccatgcgct cgtcgtactc catgccctcg gccttcatgg ccgcgatggc
     3061 gcgcgacttg agcgtgtcgg tttgcttgta gagcacggca tgagggtctt cgagggtcgc
     3121 ctccaccagc gtcagtacgt cgagcgggta ggcgggatcc tcgggctcga gcacgtccac
     3181 cgcctcgacg acgtacaacg acagcgcctg attgagcgag aagtcggatt gaaagtcctc
     3241 gttcacgcgc ggcccgtcgg cgtcgagcga aatcacgcct gcgtcccgaa gcgagcgaat
     3301 catcgcaatc gcctgacggg catggtttcg cttcgcggtc ggaccctcgt gactgctgcg
     3361 aatgagcttg cgaagcgcga ggcatccgtt gtcctcccgc tcgaagaggt tgaggatcat
     3421 cgcgttgctc acgtgaaacc gggagcgaag cggctccggc ggggattcct ggagccgggt
     3481 gaacgtctcc tcgtcccagt gtgcatatcc ccgctccgga ggctgccgca tcttgagctt
     3541 gcggagcttc ttcgcatcgc cgccggcctt tccccggagc ttcttgttct cgatggcgtg
     3601 ctcgggcgcc tgcgccacca cgctcccgag ggtgtcgaag ccgcgccggc ccgcgcgccc
     3661 cgcgatttgc ttgaagtcgc gcaccgtcag caccttcgtc tttacgccgt cgtacttgca
     3721 gagcttcgtg aagagcaccg tgcggatcgg cacgttgacc ccgaccccaa gcgtgtcggt
     3781 gccgcagatg atgcggagct tcccttcctg tgccagccgc tcgaccaggc gccgatatcg
     3841 cggaagcatc ccggcgtggt gcacgccgat cccgtggtgc aggaacttct tcagctcctt
     3901 gccgaacggg gtgtcgaagc gcgtcccgct gagctcctct ttcaagtctc gcttctcgtc
     3961 tttcgagagg aagtcgatgc tcatgaagct ctgggcctgc tcggtggccg cccgctgcgc
     4021 gaagtgcacg atgtagatcg gcgccttctc cgcttcgaga agctcgacga ccgtggcttg
     4081 caaggtgtcc tcgctgtacg agaaatcgag tggcaccggc cgatccgtgc tcttcaccag
     4141 cgtggtcgtg acaccggtga gctcggtcag cgcgtcctcg aacttctgcg tctctccgag
     4201 cgtcgcgctc atcagaagaa agcgcgcctg gggtagggtg aggagcggaa tttgccaggc
     4261 cactccgcgg tccctgtcgg cgtagtagtg aaactcgtcc atgaccacgc catcgacctc
     4321 ggcccgctct ccctcgcgca aggccatgtt cgccagaatc tccgctgtgc agcagatcac
     4381 gggcgcatcc cgattgacgg tcgcgtcccc ggtcatcatg cccacgcgct gcggtccgag
     4441 cacccggcag aggtcgaaga acttctcgct caccagcgcc ttgatgggcg ccgtataaaa
     4501 ggagcgctcg ccgagggcga tggaccggaa gtgcgccgcc aaggccacca gcgactttcc
     4561 ggacccggtg ggcgtgttga gaatcacgtt gttcccgacg aagagctcca tcaccgcttc
     4621 ttcctgcgcg gggtagagtt cgaggccctg gtcgctcacg tactcgagaa agcgggtgag
     4681 cagcgtgtcg ccgtcgacct tctcgccgag gtcatcgagg tagcggtcga gcgtgctcat
     4741 gcatgctcgc caagctcggc gtcaccgata ggggcctggg gcatgatgcg tcgagtatat
     4801 ggcaggggcc tgcaaaccgc ccgctagcat tggaaaaagc gaagccggtc agcgtgctca
     4861 agcgctgacc ggcctctgac aacgggctcc acaatgggga ggccaaggcg ggcaccattt
     4921 tcggggaacg ctttctcgcc tgactctgga tgggatggtg ggctcatccg tcgccctgcg
     4981 ttccatgccg ctggtgcccg ccttgcaccg aatgacagtc cggaacctat cggcgagccc
     5041 ggcatccgcg gcgacaaaag gagacaccga tcgcgccaat accgcctgga attcacctat
     5101 gtgcttgtaa ttctctcgcc ggcatggatc catccctggc ggtgggcgac ccatccggca
     5161 aagccagaat taactcgggc ctccgcgtct cgggcgcctg gacacgaggc gtcgtgcact
     5221 cttttcgccg tctcggcctc gatgtcgatg cgctctgcaa agagatcggc gtcggcgtcg
     5281 aggtcttcat ggacaccgac ggcagacctc cgcgcgacgc gtccggccgg ctctggcgcg
     5341 cggcgttggc ggcctcaggc gaccgtcacc tcggcctgag tgcagccgat gcctgggagg
     5401 cccgcgcgga tcacctcgtc gtcctcttgc tgaccagcgc caccagtctc ggccagggcc
     5461 tcgaagcggg cctccgctac caggagcttc tgtcgcacgg ccgggtcatc accctcggga
     5521 ctcatcccga gctccacgtg ctgcacatca acaagatcga gcacgagctc ccgatcacgg
     5581 tccacgaggt cgagttcatc acagtcgtcg tgatgaagat gcttcgcttc gcaacggacg
     5641 gcgcgttcaa ggccgaggag attcacttcg agcatccgta ccgaggcaac attcagaagt
     5701 acacgcgggc tttcgggtgt tcggtgctct tcggtcaaga gaagaccgcc atctttgccg
     5761 acgatgcgtc ctggaccctt ccactcgcgc acggcaatcc ggcgcttcac ggccagctca
     5821 agagtgtcgc cgcgggtctt catgcgacgc tcgagtccca ccacttcatc gacacggtgc
     5881 gcgagcgcat caagatcttg ctcccgcgcg gccaaaccag catcgaagcg gttgcctcgg
     5941 cgcttcacat gacgcctcga acgctgcaac ggcggcttca agaagaggat acgacgttcc
     6001 gggtcctgct cgacgcggtt cggaagtcga ttctcgtcga atgcttcgag cggaatcagg
     6061 caccccggga gatcatgcgg cacgccgggt acaccaacgc gaagagcttt cgccgcgccc
     6121 tcaagcgttg gaatttctcg aacgccgagg acggcctgcg ttcctagctc agggctcccg
     6181 aacctcgttc gcggtccagc catcgtttcg gtgcaccgtg ttccggtcgc gaaactcctt
     6241 gtctgcctcg tcccagctcc gctttgcctc tccggctctc gatccgatct tcgagcgttt
     6301 ccaggagacc cattccgtgc attggcgcaa attgggctgc gcggggagct gctcgcgaac
     6361 cgcggcagga aaggcttcga gcggctggtc ctctcgcctc aggacctcga tttcgaccgg
     6421 gctcttcaca ttcactcgcg ggcgcaggcg aaccacgtag gtcttccccg cgctcaggtt
     6481 gacctccagg agctgcacca cgggcgggga catgccggtc accagcatga gcatctgctt
     6541 tccaggccag atcgctgccg ccatctgcca gtcgttttcg agcagggcga tacacttgcc
     6601 ggccggagtc accacccgaa tccccgtttc gctcgcttga cgatgccggg gcctatcgaa
     6661 gatcagcagc gcctggtcgg ctggggccac gtatgaagag tccggcgtct tcgggatctg
     6721 cgcctgggcc gtgaggccga gcgagagcac ggcgagcggg agccacgcaa aggagatacg
     6781 gggtttcttc gcctgtgcac gcattctgtg aggtatctcg catgtcacct ctccggcatc
     6841 cagtgggatt cgagcgttcc atgcaagttc cgcacggctt tctgcgtcga cgactctcag
     6901 gggcaagtgc ttggattttt ggcaatactg tatccgtgcg aacttcaggg gtggggagtc
     6961 aggcgccagc gctgccggcg accccgggaa atcaaatacc gcagatcggc gatgtcttgg
     7021 actcgcgcta tcgcattaca ggggttctcg gctccggcgg gatgggttgc gtctacctcg
     7081 cggagcacgt ttcgattcag cgacccttgg ccctcaagct tttgcaccca gaggttggtg
     7141 acagcgaaga ggtcaacaag cgcttcgagc gtgaggcctt tgccatcggt cgagtcgacc
     7201 atccgaactg cgtcgacgtc tcggacttcg gcaagcttca agacggcacg cactacatgg
     7261 tgctggagct gctcgatggt gtcctgcttt cagacttgct cgaacggaaa cagcgtctcg
     7321 gttggaagcg cgcgcttcac atcgggcggc acgttctgag cgcgctcgat tatgcgcatg
     7381 acgcgggaat catccaccgc gacatcaaac ccgagaacgt gattctcgtc gagcaggatg
     7441 gcgaccccga tttcgcaaag atcctcgact tcgggatcgc caagtttcgc gacgacgcga
     7501 agcccgaggt gcagaccggg ccgctcggca acgacgccaa gctcactcag cacggtgttg
     7561 cgattggaac gccgacctac atctcgcccg agcaggccta cgggcagtcc atcgatggcc
     7621 gcgccgatct ctattcgctc tcggtgatgc tctacgaaat gattgcgggc gtgcctccct
     7681 tcgatgcaga cgaggtgggc acgcttctcc gcatgcacgt ttcggccgag gttccgcgct
     7741 tcggggacat tgcgcccgag gtcgacgttc ctgcagaggt cgaagcgctg atccgcgacg
     7801 gcctcgccaa aaaggccgat gaacggatcg caactgccca tgcgtacatc gcgcgcatcg
     7861 acgcgctcgt tccgctcgcg agcggcacgg ggtccggcct tcgcgagctt ccgtcgattc
     7921 gggtcgctgc tgtgcgtgac acgaccgcga cagcgggtgt gcgcgcgctg cgaaggaagt
     7981 ggccgtccaa gtggatagcc gccgctgtgc tcttcctcgt tgctgggacg ggccttgcgg
     8041 ttcttggctt ccgtagcgac ggtcccaact atcttcccga cgggaatata ttgccgttgt
     8101 cgtctcctgc gcacgggccc gaggccgagg tggcagcgga aatgctcgcc cagggccgcg
     8161 ccgaggaagc cgcctcgtac ctcatggagc atcgcgcgga tctacaagag gagccctacg
     8221 cgcagatggt tctcggtcac gcccaggcga gtgcgcagcg aagcattcag gctctcaatg
     8281 cctatgcgaa agcgatccag ctcgagccca agctcgccaa agacaagctc atgcgcacca
     8341 acatcgagct catgctcgcc aagaaggctc ccggtatcgt cgatacggca ctcgagctcc
     8401 tggggacctt ggtgtcggag gccgacgacg cgactgcagc ggatcagctg atcgatctcg
     8461 cgtcctcgtc gaaggtgacg cgtcggcgcc acaaggccat gagcgtcgcg gacagcgtgg
     8521 gtctcagcga ccgggtcgac cgtctgggct cctatttgct cgacctccag ctcggagaat
     8581 cctgcccagt tcgcaagtcg gcggtcgcca atctccgtgc gctcggagac aagaaggcga
     8641 tcccagagct ccgcaaagca cggaagcgca tccggaccga gggcggcttg atcaagcgaa
     8701 aggtcaacac caacgcctgc ctccgggccg acgcggccga ggcgatccgg tacttgcaga
     8761 gtctctagcg cttcgaggtc cctgggccga gctcaattgt gagcttgctt acagtgtgcg
     8821 gaactcagcg ctgaccctag gccccagcct ggacacgacg aaccggtgtt cagtatgctc
     8881 gccgtccgtc cgggcgtggc ctcttaaagg actaggtgaa gacccatgaa atccagcgca
     8941 tccattctca cgattcttgc ttccgtcatg gttctccaga gctgtgggag cgacaccagc
     9001 caagggagca ccgagcttgc ggccgttctc gatgctgcag ctgaccgcga gggcgtggcg
     9061 cctggtgact gtcgcgacgc gtgtctggcc gcggcgctcg aggttttcga cgcctgcgcg
     9121 gccgagagcg atgaccgcga ggcctgcttc gggcaagcga tccacgcctt cttcgaatgc
     9181 gcaaagacct gcgagccgcc gagctgcgaa gcccgctgcg agaacgcggc aagcgagatg
     9241 tatgaggcct gcgtcgccgg aggtggcacc gaagatgagt gcgcgatggc agcgcgcgag
     9301 tcgctcgcgg cctgcaaggc cgagcactgc gaagcgggcc tgacctgcga agacgaatgc
     9361 ggaatgctgg cggagcaagt ctacgacgtc tgtatggaga agctcgactc tgaggatcga
     9421 tgcgccgaaa tcgcgggcgg agctgcgcgg ctctgcaaga agcactgcga aggttatcaa
     9481 tgcccctgcc acgacgacga ctgcgatgac gaggacagcg acagtgacag tgacagtgac
     9541 agcgacagcg acagtgacag cgacagtgat tcggacagcg acagtgacag cgattccgac
     9601 aaagacaagg actgccacga ggggcacccc gactgggaga agagcagcgg gaagtgcaag
     9661 tagcctcacc tcgctcgtga agccgtcagc aacacgcatc gttcgactgt gctaggtatc
     9721 gcgtcgttcg atgcgcgtgc tgagcttcaa tgccaatggg attcgtgctg ccgcccgaaa
     9781 aggcttctac gattggctgc ccgagcagca ggcggacttc gtctgccttc aagaaatcaa
     9841 ggcacgaccc gagcaactga gcacagccga gctcgagcct gccggatacc agtgtttcta
     9901 cgagcccggt gagcgaaagg ggcacagcgg tgtcgcaatc ctcagccggg aggagccgga
     9961 tgaggtcatc tacggattcg gctcacgcga gttcgatccg gaagggcggt acatcgaggc
    10021 gcgcttcggg aacctcagca tcgtctcggt ttacgttcct tcgggcacca gcaacgaaac
    10081 ccgcctcgcc gccaagttcc gattcatgaa gcagttctcg aaacaccttc gcaggcttcg
    10141 caagagcggc cggcagtacg tgctcagcgg cgattggaac gtggcccaca aggaaatcga
    10201 cctcaagaac tacaaatcga accagaagaa ctcgggcttc cttcccgagg agcgcgcctg
    10261 gctcgaccag gtgttcgggc gcatcggctt cgtcgacgca tttcgcgagg tcaacccgga
    10321 gcccgagcag tacacctggt ggtcgaaccg tcatcccacc acccgcgacc gcaacatcgg
    10381 ttggcgcttg gactaccaag tggtcacccc cgagctcggc gcacgcgctg tgtcggcatc
    10441 aatcgccgta gaaccgcggt tttcagacca cgcgccgctg ctgatcgagt accgggagag
    10501 ctgaccgccg cctcgtgcta tccctagacg cgatgggagc ttatctctat ccgatcagcc
    10561 tcgccgggat ctcggtcttc gtgtttgcgc tggagcgctt ctttccctgg cgtcccgagc
    10621 aaaagcagtt tcggcccaag ctctggtccg acttcattca cctggtcttc aacgggcatt
    10681 tcctcggcct cttgctcgcg ggcttggcga gcacctgggt cttgccttcg gtcgaccgat
    10741 ggctttcggc tgagggactg acggacgcgg tgtatcgcaa cgccgccgcc gactggccgc
    10801 tctgggcgca gatcatcgtc gcgctcctcg tgctcgactt ccttcaatgg tgtgtgcaca
    10861 acctcttgca ccgcgtccct ttcctctggg agctgcacaa ggcgcaccac agcgtgaagg
    10921 acggagagat ggactggatc gtctcgttcc gtttttcctg gctcgaggtc atcgtctaca
    10981 agtcggtcct ctacctgccg ctggtcttct tcggcttccg atgggaagcc ctgatgttcc
    11041 acgcgatttt cggcaccctg atcggccacc tcaaccactc gaacctgaac ctcggccatg
    11101 gtccctggcg ttacgtcctc aacagccccc ggatgcacat ctggcatcac gactacgacg
    11161 gcaccgcgaa gaccaccgtc aactacggca tcatcttcag ctgctgggac tggatcttcg
    11221 gcactgcgaa gatgcccgcc gacccaccgg cgcacctggg cttcggcggc gtcgaaacct
    11281 tccccgacaa cttcttcggc caggagatct ggccgcttcc gaagctcttg ccgcgcgtcc
    11341 acagcacggt cgtctggtcc atcatcggcg cgcttgtgct ggcggcggct tggtaccttc
    11401 gcgagccacg caggctcggt ccggaaacgc cgatgctggg cgagtgggcg gcggcttcac
    11461 agccgagcgg cgtgttcccg gcgttcgact cgtattcgcc aagtccggcg gaggctgaag
    11521 cggagattgc gcgcttcgga gagcaggccc gcgccgatgg gtacgcggat ccccagttga
    11581 tcgtgagcgt ggcagagctg gccaaggcgc taggtgcgcc gacattggtg ctgctcgacg
    11641 tgcgccccga ggcgcgcttt gccgtgggac atatcccggg tgcccgccgt ctcgaccgag
    11701 aagactattc gagcggcggc gagatcccgg gcctctcgcg gcctgcgccc gagctcgagc
    11761 agcgattgcg tgcgctcggt gttcaccgcg atagcacggt ggtcctctac ggagacggcg
    11821 gcccggaacc ctatcgactc tggtggacct tgcgcgtgac gaccgcgctc gagacgcgcc
    11881 tcctcgacgg agggctgcaa gcctggaagt ccgcgggcca tcgtgtcgcg ggcggtgaag
    11941 ggttcgaagt tgcaccaggt gatgtgcagc tcggcgcacc ggcatcggct cccatccagc
    12001 gctgggcgga gactcgagcg tttatggccg agcatcccgg cgtgcgcctc ctcgatacgc
    12061 ggagcgccgt cgagttcgac gggagcgaga agcatcccaa ggcttcgcgg gctggccgca
    12121 ttccgggcgc aacgcatctc gactgggtcg aggtcttccg cgacgaggac gaagaccacc
    12181 gactgaggct gcccgcggag ctgcgcgcct tgctcgaagc gcgcggcgtc gccgagggca
    12241 cgccggtggt gacctactgc cagtcgggca cc
//
LOCUS       DASRZF010000246         2548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3963599, whole genome shotgun sequence.
ACCESSION   DASRZF010000246 DASRZF010000000
VERSION     DASRZF010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3963599"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..587)
                     /locus_tag="VFG22_10715"
     CDS             complement(48..587)
                     /locus_tag="VFG22_10715"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HET6416756.1"
                     /translation="MLRIALVLSVLAFTPACDRSKINESTWKEAGAEAVLPFKQGLKQ
                     ALLKGMEGDPIQAISACRIEAPKIADSLSKGNLQMGRTSQKLRNPKNAPKPWMEPFLE
                     AYASDPESREPKVVLIDQNTVGYVEPIFVQPLCLTCHGTELAPELEAAIEQQYPSDEA
                     TGYVAGDLRGIFWAELPRE"
     gene            673..1326
                     /locus_tag="VFG22_10720"
     CDS             673..1326
                     /locus_tag="VFG22_10720"
                     /inference="COORDINATES: protein motif:HMM:NF043005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCE4755 family polysaccharide monooxygenase-like
                     protein"
                     /protein_id="HET6416757.1"
                     /translation="MVQFCLALLLGLVALAPVEAKAHLGLSAPASRYGPDTLKMGPCG
                     VSGGERTEIVTYYEPGETIEVSWDEYIDHPGHYRIAFDEDGDDDFVDPATMMELYSNE
                     TVLLDGIDDKGPGERDYVATVTLPDLVCDNCTLQVIQVMYDKPPYTTPGNDIYYQCAD
                     LVLRVGGAPPDAGIEPGAQSSTGCSVGVIDGGSSVGLIGLFFLWLARRLARQRRLPV"
     gene            1337..2200
                     /gene="metF"
                     /locus_tag="VFG22_10725"
     CDS             1337..2200
                     /gene="metF"
                     /locus_tag="VFG22_10725"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HET6416758.1"
                     /translation="MRIADSYAAGRGPVLSLEFFTPRDEAGRASLFLAIERLMAIEPG
                     FVSITCGAAGTTRERTADLAIEVQREFGLTAMAHLVCTGTTQTELEETLRHMRAEGID
                     NILALRGDPPKGVRDWTPVEGGFRHANELAEFVRKRFSLGIGGACYPEKHHEAPSLDV
                     DIQNLRLKVEAGAEFLITQLFFDNEKFWAFVEKVRAQGIDVPIVPGIMPVLNLTNLQR
                     IASLSPGTTIPAELETAFRDAGDDEEAALQAGVAFAAKQCRALLAGGAPGIHFYTLNR
                     SKATLQTVAQL"
     gene            complement(2239..>2548)
                     /locus_tag="VFG22_10730"
     CDS             complement(2239..>2548)
                     /locus_tag="VFG22_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416759.1"
                     /translation="GVWVTPWETLYYGGIGVAAAGFVIGVPTGFVYHVRLYQVLHPRG
                     ELPGGWYWRPIRYNSCLRPEERRGVMSWCYLGGFGFVVICLGLLIMAAGVSMALIRGA
                     "
BASE COUNT          482 a          815 c          767 g          484 t
ORIGIN      
        1 atcgaacccg agcgcgtcgc ggtgatgggg tgaggcccac ccccggatta ctcgcgaggt
       61 agctccgccc agaatatccc gcggaggtcc ccggccacat atcccgtggc ctcgtccgat
      121 ggatactgct gctcgatcgc cgcctcgagc tcgggagcaa gctccgtacc gtggcaggtc
      181 aggcaaagcg gttgaacgaa gatcggctcg acgtagccca ccgtattctg gtcgatcaag
      241 acgaccttgg gctcacgact ctcgggatct gacgcgtacg cctcgagaaa cggctccatc
      301 catggcttcg gcgcattctt cggattgcgt agcttctggc tcgtacgccc catctggaga
      361 ttgcccttgc tcagcgagtc ggcgatcttt ggcgcttcga ttcggcaagc gctgatcgct
      421 tggatcgggt cgccctccat gcctttcagc agggcttgtt tgagcccctg cttgaacggg
      481 agcacggcct cagcgcccgc ctctttccac gtcgattcgt tgattttcga acggtcacag
      541 gccggagtga aggccagaac cgacaagacc agggcgatgc gcaacatgct ctacatctac
      601 tcccatgcgg gtgctcggat caagccgcca cgcattcgtg cccgctcgac ctagctacgt
      661 tagcatgtgg gaatggttca attctgcctc gctctcctcc tcgggctcgt cgcactcgcg
      721 cccgtcgaag cgaaagccca tctcggcttg agcgcgccgg cctcgcgcta tggacccgat
      781 actctgaaga tggggccttg cggggtttcg ggcggggagc gcactgaaat cgtgacctac
      841 tacgagcccg gcgagacgat cgaagtgtct tgggacgagt acatcgacca ccccggtcac
      901 taccggattg ccttcgacga agacggtgac gacgatttcg tcgatcccgc aacgatgatg
      961 gagctctatt ccaacgaaac tgttttgctc gatggcatcg acgacaaggg accgggcgag
     1021 cgcgactacg tcgccactgt gaccctgcct gacctggttt gcgacaattg taccttgcag
     1081 gtgattcagg tgatgtacga caaaccgcct tacacgacgc ccgggaatga catctactat
     1141 cagtgtgcgg acttggtgct tcgggtcggc ggagcgccgc cggatgcggg catcgaacct
     1201 ggtgcccaaa gcagcacggg ttgcagtgtt ggagtcatcg atggggggag ctcggtaggc
     1261 ttgatcggtc tcttcttcct gtggctggct cgccgtcttg cccggcagcg ccggttgccc
     1321 gtatagagag gcgcctatgc gaatcgccga ctcatacgcc gcaggccgcg gacccgttct
     1381 ctcgctagag ttcttcacac ccagggacga ggctggtcgc gcttctcttt tcctggcgat
     1441 cgagcgactc atggcgatcg agcctggctt cgtttcaatc acttgcggtg cagctggcac
     1501 cacgcgtgag cgaacggccg acctcgcgat cgaagtgcaa cgagaattcg ggctgaccgc
     1561 gatggcgcat ctggtttgca ccggcacgac ccagactgag ctcgaggaaa cactccgcca
     1621 catgcgagct gagggcatcg acaacatcct cgctctgcgc ggcgacccac cgaagggtgt
     1681 ccgcgattgg acgccggtcg aaggaggctt tcgccacgcc aacgagctgg cagagttcgt
     1741 cagaaagcgt ttctcgctcg ggatcggggg tgcatgctac ccagagaagc accacgaggc
     1801 cccgagcctg gacgtcgata ttcagaacct tcggctgaaa gtcgaggccg gcgcagaatt
     1861 cctgatcacg cagttgttct tcgacaatga gaagttctgg gcgttcgtcg aaaaggtccg
     1921 cgcgcaaggc atcgatgtgc cgatcgttcc tgggatcatg cccgtcctaa atctgacgaa
     1981 cctgcagcgc atcgcgtccc tgtccccggg aaccacgatt ccggcggagc tcgaaaccgc
     2041 ctttcgcgat gcaggggacg acgaagaggc agcgctgcag gcgggggtcg cgttcgctgc
     2101 gaaacagtgc cgtgccctac tcgccggcgg cgccccaggc attcacttct atacgctcaa
     2161 ccgatccaaa gcgacgctgc aaaccgtcgc gcaactctaa ctcgggtgcg cctggccccc
     2221 gctttccggg ccggcctgct aggcgccacg gatcagtgcc atcgagaccc ccgccgccat
     2281 gatcagaagc ccgaggcaga tcacgacgaa tccaaaacca cccaggtagc accagctcat
     2341 cacaccacgc cgttcctcag gtcgtaggca gctgttgtag cggatggggc gccagtacca
     2401 gccccccggc agctctcccc gtggatgcaa gacctgatag agccggacgt gatagacgaa
     2461 tcccgttggg acgccgatga cgaatcctgc ggccgcaacc ccgatgccac cgtagtacag
     2521 cgtttcccag ggggtgaccc agacgccg
//
LOCUS       DASRZF010000247         2243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4989031, whole genome shotgun sequence.
ACCESSION   DASRZF010000247 DASRZF010000000
VERSION     DASRZF010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4989031"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..414
                     /locus_tag="VFG22_10735"
     CDS             88..414
                     /locus_tag="VFG22_10735"
                     /inference="COORDINATES: protein motif:HMM:NF017451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HET6416760.1"
                     /translation="MGLIIQQRALEAASGLRRVMPLVKKRDRSLFDQIHRAMNSVVLN
                     IAEADGNDPGTAKARFASACGSAKEVRAGLQLGVAYGYFPGAKVQAIDTALDEVCAMS
                     WRLSGR"
     gene            complement(411..740)
                     /locus_tag="VFG22_10740"
     CDS             complement(411..740)
                     /locus_tag="VFG22_10740"
                     /inference="COORDINATES: protein motif:HMM:NF017451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HET6416761.1"
                     /translation="MKKNVYERARDVVRDLVPVLRKIRECDKSLADQLKRAAQSVVLN
                     IAEGRGSDAGNARARFSTACGSAKEVRAALHVASDWGYIESRTAARLDRKLDEVCAIT
                     WCLGRSR"
     gene            complement(927..1931)
                     /locus_tag="VFG22_10745"
     CDS             complement(927..1931)
                     /locus_tag="VFG22_10745"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6416762.1"
                     /translation="MSTKPSIRPEAMASHGLVKFVRQHLVSTEYAEIVDLRGKTIVVT
                     GASPGSLGFATAETLAKWGASVIVTMRSNPDSAADAIRSRLETREDGARVHAHPLDLG
                     SRESVLHFVRWFEATQGDRLDVLVNNAGVHLDLLSRWKEPQQTDDGFEIHWRINYLGT
                     AQLTQLLLPLLEKTGAKTGAARVVNVSSKLHVRGSNDDLFGQARPYNSWNAYGNSKLA
                     LIHMANELQRRYATQSNLSAYSLHPGAVFTHVADKGLEGTGFAEKVRNALWPVEAFFL
                     KTPTEGAQTQIHCATWPKLQGGLYYEDCNPSEPSDDAKDADVATRLWDATLRWIEEGG
                     "
     gene            complement(1951..>2243)
                     /locus_tag="VFG22_10750"
     CDS             complement(1951..>2243)
                     /locus_tag="VFG22_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416763.1"
                     /translation="RALIETDIAFHHVGIYQQGMEQLAASITAAGYPEVVRGGISIGS
                     GLSIDFRYFDSRHDHGIYLEILDFSVMGLELNLEPLIRGYAAAKSAIRKLGR"
BASE COUNT          395 a          695 c          714 g          439 t
ORIGIN      
        1 gtcatcgcga tctccttttt gcctgggtgt gggggcgggc ttcgcccttt cggggaaccc
       61 cacacccagg caaaaaggag atcactcatg ggacttatca ttcagcaacg ggcgctcgag
      121 gcagcatcgg gacttcggcg ggtgatgccg cttgtgaaga agcgggatcg gtcgctcttt
      181 gatcagattc atcgggcgat gaatagcgtc gttctcaaca tcgccgaggc cgatggaaac
      241 gatccaggaa cggcgaaggc gcggttcgcg tcggcatgcg gctccgccaa agaagtgcgc
      301 gcgggacttc agctcggcgt cgcctacgga tacttccccg gcgcgaaggt ccaagccatt
      361 gacaccgcgc tcgacgaagt ctgcgcgatg tcatggaggc tttcgggccg ctagcggctt
      421 cggcccaaac accaggtgat cgcgcacacc tcgtcgagct tccggtcgag gcgcgcggcc
      481 gtccgagact cgatgtagcc ccagtcgctc gcgacgtgga gcgccgcccg gacttccttg
      541 gcggacccac aggccgtaga gaaccgagcc cgcgcattcc cggcgtcact accgcgcccc
      601 tccgcgatgt tgagcaccac gctctgcgca gcccgcttca gttgatccgc aagcgacttg
      661 tcacactcac gaatcttccg caaaaccgga acaagatccc gcacgacgtc tcgcgccctc
      721 tcgtagacat tcttcttcat gagcgactcc ttggaatggg ttgtggcctc acctgtgcgg
      781 ccacaaccca ttccaaggac aagcgatgac aagtagaaat gcgaaggcac cgacctcagc
      841 gccagcgcca gtgccagtgt cagtgtcagt gtcagtgcca gtgccagtgc cagcgccagt
      901 gtcggtgtcg gtgtcagcga ccgcgatcag ccgccctctt cgatccagcg cagggtggca
      961 tcccagagcc gtgtggctac gtcagcgtcc tttgcgtcgt cgcttggctc gctcggattg
     1021 cagtcttcgt agtagaggcc gccttggagc tttggccagg tggcgcagtg gatctgcgtc
     1081 tgcgcgcctt cggttggtgt cttgaggaag aacgcctcga cgggccagag cgcgttgcgc
     1141 accttctccg cgaagccggt gccctcgagg cccttgtcgg cgacgtgggt gaagacggcg
     1201 ccgggatgca ggctgtatgc gctgaggttc gattgggtcg cgtagcggcg ctgaagctcg
     1261 ttggccatgt ggatcagggc gagcttcgag tttccgtacg cgttccagga gttgtagggc
     1321 cgcgcttgtc cgaagaggtc gtcgttggat cccctcacgt gcaacttcga cgagacgttg
     1381 accacgcgcg cagccccggt cttggcgcct gtcttctcga ggagcggcag caagagctgc
     1441 gtgagctgcg cagtgccgag gtagttgatg cgccaatgga tctcgaagcc atcatctgtt
     1501 tgctgcggct ccttccaacg tgacaacaag tccaggtgaa cgccggcgtt gttgaccagc
     1561 acgtcgagcc tgtcgccctg tgtcgcctcg aaccagcgca cgaagtgaag caccgattca
     1621 cgtgaaccga ggtcgagggg atgcgcgtgc actcgagcgc catcctcgcg cgtctctagg
     1681 cgtgagcgaa tcgcatccgc cgcggagtcc gggttcgatc gcatggtcac gatcacgctg
     1741 gccccccatt ttgccagtgt ctcggcggtc gcaaagccga gggagcccgg gctcgcaccg
     1801 gtgacgacga tcgtcttccc ccgaaggtcg acgatctcgg catactcggt gctcaccaag
     1861 tgctggcgca cgaacttcac gagcccgtgt gaggccatcg cttctggacg tatggagggt
     1921 ttcgtggaca tcgagtgccg tcgaccttgc ttagcgaccg agcttgcgga ttgcgctctt
     1981 ggcggcagca tagcctcgaa tgagcggttc caggttgagc tccaggccca tcacgctgaa
     2041 gtccaggatt tctaggtaga tcccatggtc gtggcgcgag tcgaagtagc ggaagtcgat
     2101 cgacaatccg ctcccgatcg agattccacc acggaccact tcagggtatc cggcagcggt
     2161 gatcgatgcg gcgagctgct ccatgccctg ctggtagatc ccgacatgat ggaacgcgat
     2221 gtcggtctcg atgagcgcgc gcg
//
LOCUS       DASRZF010000248         8812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_868961, whole genome shotgun sequence.
ACCESSION   DASRZF010000248 DASRZF010000000
VERSION     DASRZF010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 8812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8812
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868961"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="VFG22_10755"
     CDS             complement(<1..993)
                     /locus_tag="VFG22_10755"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF026066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HET6416764.1"
                     /translation="MSVRNAEIAEILDRMADLLEIQGANPFRVRAYRNASLTVAGLAR
                     SLADMLAVSEDLSKLPDIGKDLASKIREIVDTGRLAALEELEKDVPAGLVELTRLPGL
                     GPKRVKSLHESLGIHDLEGLERAARKHRIRELPGFGAKTEQRIVEALARHEQNEGRHK
                     LADVEGIADAYVEYLEAVAGVNRVVVAGSYRRRQETVGDLDILVTCKKGTPVMKAFVE
                     YEAVDAVSSRGTTRSTVVLRSGLQVDLRVVPEISYGAALYYFTGSKAHNVAVRTIAVK
                     KKLKINEYGVFKGKQRVAGRTEEELFKTVGLRYIEPELRENRGEIEAAQKNRLPH"
     gene            1158..2147
                     /locus_tag="VFG22_10760"
     CDS             1158..2147
                     /locus_tag="VFG22_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416765.1"
                     /translation="MSDRNLALSLWVGAALLAAGCSGNSVSVPCYSALLIDLDVSVEG
                     EEQSILLDQVLWTLTGNGMAPMSGAIDTSAPGATPSVEVFGLNPGSYMIDLEASADDG
                     ETRCRGSAPFDVTAGVSTEVAVLLRCKVGQRFGAVRVNGKLNICSELTKAVVAPLRTS
                     IGNRIAVLSQAEDEEGDSIAYAWTADSGSFEDSSAVDTFYTCEETGDHSLTVSVSDDG
                     FAYCVDSWTVAIRCVDEGGTGGTGGNGSGGMGGSGATGGSGGAGGAGATGGAGGTGGA
                     GATGGSGGAGGTGASGGSGGAGGTGATGGTGGSGGAGGTGGGICEITISVTDS"
     gene            complement(2178..3854)
                     /locus_tag="VFG22_10765"
     CDS             complement(2178..3854)
                     /locus_tag="VFG22_10765"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET6416766.1"
                     /translation="MFRWRLAVALLGVTAGLVFSSSAYAQTPPQFDLNQFRPSQLATD
                     GFAVSDADGQGHLRFGVQLYFDFARDPLELKVTNGPIPDQRLGLVHSQLTGHLTWNLG
                     LWDRLVIFMDLPYSFMLNDNLSQSAVDFLDSIGQGPLIPRGSGLGDLYVGARGVLYGT
                     RENVFQLAAQAILTANTASASRPQQNYLGEPNASPHIGGWFELLGTFNAGDYFRIPLN
                     VGYKTSFQQDLPSLEIGNQLTFGAAVQVLLGQERFMITVEAFGRTAADTATGFGGRQE
                     TPVELLGGFKYLNQKGVTLGMAGTGGVTSGYGNPDWRLIGMLAYTMPGKAKETPVDTD
                     GDGIVDEDDECPDEAEDFDRFQDEDGCPDLDNDADGILDLDDACPNEPGPPENDGCPD
                     TDRDGDGVPDRIDNCPDEPGPVENQGCPEEQHVVIEESQLEILDKIYFNVDSANLQRR
                     SYPVLDNVAEVLNAHPEISLIRVEGHTDSTGSDGYNLRLSQRRVDSVVRYLVEKGGVS
                     QDRLVAEGFGETRPLVPNARTRTELAQNRRVELHIAEGEPEEPEDAPSDP"
     gene            complement(3984..5153)
                     /locus_tag="VFG22_10770"
     CDS             complement(3984..5153)
                     /locus_tag="VFG22_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416767.1"
                     /translation="MTDRSARMRVVDRFVDHGTPISFRELCARVLDEQHDYLILDLDK
                     TTHLGRNLGELLAWELCAYEVYGEPEDGRQLHRWFGGRVLLDARRPILLVRYLGAGAK
                     RWAMAGIHYLIWGKLASHISWLRRLAYSRFGSHPTSAVQRRPQMVALAHLEAVDESVL
                     RRLARRVWDRQSPDQVISREDLDWVRARAPGIRIILSSASPKAMLDVAVEKLGADGAT
                     YSTVDRINSGRAKIERLREIDPRIFTPGVKVLAMSDTSYGEDHCWTEHFDCVVDINSP
                     TPFSAIVPQASPVQAIHSATLLTREERQRRSHGDSGYLDPRRKGRRARATLRLDGEAL
                     DALLGDLVSEMNQMAAAPSPFGRGWELAHALAERASKSRARLTLPWAVAAVPTES"
     gene            complement(5463..7955)
                     /locus_tag="VFG22_10775"
     CDS             complement(5463..7955)
                     /locus_tag="VFG22_10775"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET6416768.1"
                     /translation="MRNLPTTIAALVACIAMHASVRAEDLEPDGEAPVLTAPSLPANG
                     HMHPPYPAGVDGKPARVLLRITIDSEGRVADANVLTVDREDQQADQFAGLALQYVREL
                     TFEPAMRDGDPVPAIVQFEVLFDPPEHARGAVNESAAGERAKPEGESPREPSFSATAV
                     VAPSDISASSIDISGDEIRLRPYVSTGELLNAAPGFYVIQHAGGGKANQYFLRGFDAD
                     HGTDVAFFLDGVPLNWVSHGHGQGYTDLNFIIPELIQRVEVRKGPYQAEYGDFATAGA
                     INLVLDDEKPMSSLTVGGGTYRTFRGVGIISPKLDKVKPLIAAEVFGTDGPFENGEDL
                     LRVNIFARTTFELRGETRLTLTGMAYLGDWNASGQIPLRAVESGDLDWFGSIDKNEGG
                     QSQRYSLYLSVQSPAKPGAQGKRGIGGEGLNLTGWVTLSRFALYSNFTFFANDPVNGD
                     MIRQGDDRTAFGLRASYGFAHRFDRVTLKSRMGASFRFDAIDNTLQTAPAREILETQV
                     DADISEGGLGLFGEEELAWKWLRLIGGLRFEYFGFDVTDRLEDRDTLGTMSSGEEAQA
                     IVLPKASLIVHPVEPLELFFNFGRGFHSNDARGVVLGTDPATPLSAALGWELGARVLA
                     WERFQMSTAFFWLNLDSEVVWIGDEGTTEASGETQRFGLEAQFRLEILPWLLADLDAT
                     WVRAEFVNNPGNANAVALAPELLISGGLSARDTRTGLSGRIGIFYIADRPATEDRFLT
                     ADGFVRVDASFGWENRRFAVKAQVLNLINTRWRQAQFATVGRLPGEDGPEDCPSGTRP
                     ISEPDGTFVGCEDIHFTPGWPIFVQLMATVKF"
     gene            8190..8354
                     /locus_tag="VFG22_10780"
     CDS             8190..8354
                     /locus_tag="VFG22_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416769.1"
                     /translation="MRNAAFTMLGWGFLFLLGVTLMPMTMLRIMVMAVIVGIERVVKN
                     LMGQRNDVEA"
     gene            complement(8566..>8812)
                     /locus_tag="VFG22_10785"
     CDS             complement(8566..>8812)
                     /locus_tag="VFG22_10785"
                     /inference="COORDINATES: protein motif:HMM:NF014522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HET6416770.1"
                     /translation="LAEPGLVTLEQILLYHVVAGENSAKDLIRERRVETLEGSTVYVW
                     WFFGKVFVNRERVIDANLRAGTGIAHVIDGVLLPPSH"
BASE COUNT         1604 a         2779 c         2713 g         1716 t
ORIGIN      
        1 atgcggcagc ctgttcttct gcgcggcttc gatctcgccc cggttctccc gcagctcggg
       61 ttcgatatag cgaagtccca ccgtcttgaa gagctcctct tccgttctac ccgccactcg
      121 ctgtttgccc ttgaagacgc cgtattcgtt gatcttgagc ttcttcttca ccgcgatcgt
      181 gcgcaccgcg acgttgtgtg ccttcgagcc ggtgaagtag tagagagccg cgccgtagga
      241 gatttctgga acgacgcgca ggtcgacttg aagtccggac cgcagaacta cggtcgaacg
      301 ggtcgtgccc ctcgagctca ccgcatccac cgcctcgtac tcgacgaacg ccttcatcac
      361 cggcgtcccc ttcttgcacg tgaccaggat gtccaggtct cccacggttt cctgacgtcg
      421 acgatagctt cccgcgacga cgacgcgatt cacgccagcc acggcttcca ggtattcgac
      481 atacgcgtct gcaatgccct cgacgtcggc aagcttgtga cgtccctcgt tctgctcatg
      541 cctcgctagc gcctcgacga ttcgttgctc cgtcttcgcg ccgaagcccg gcagctcgcg
      601 gatccggtgt tttcgagcgg cgcgttcgag accttcgaga tcgtgaatgc cgagggattc
      661 gtgcaagctc ttgacgcgct tcggccccaa gcctggcaag cgagtcagct cgacgagccc
      721 agcgggtacg tctttttcca gctcttcgag tgcagcgaga cggcctgtgt cgacgatctc
      781 tcgaatcttg ctggcgagat cctttccgat gtcgggaagc ttcgagaggt cctcgctcac
      841 ggccagcata tcggcgagac ttcgcgctag gccagccacg gtcagggacg cgttccggta
      901 agcgcggacc ctgaagggat tggccccctg gatctccaag agatccgcca tgcgatctaa
      961 gatttcagcg atctcggcgt tacgaacgga catggctctc gcgtgctgac ggtagcagcc
     1021 gcgctcgagg tatgggatcg ctcaatctct tgcgcctaca gatgctgagc gttccgtgtt
     1081 tacgaacgcc tggccccggg gtagcctcgc tgcccttgtc ctcatttgcc ctccagcgct
     1141 ctgaagaagg atccatcgtg tccgaccgca acttggcctt gtccctctgg gtgggcgccg
     1201 cgcttctcgc ggccggctgc agtggcaact ccgtctcggt cccttgctac tccgcgttgc
     1261 tcatcgatct cgatgtcagc gtggaggggg aagagcagag catcctcctc gaccaggttc
     1321 tctggaccct cacaggcaac ggaatggccc cgatgtcagg cgccatcgac accagcgccc
     1381 cgggtgccac accttcggtc gaggttttcg ggctgaaccc ggggagctac atgatcgatc
     1441 tcgaagccag cgcggatgat ggcgagaccc gctgccgagg gtcggcgccg ttcgacgtca
     1501 cggcaggcgt ttcaaccgag gtagcggtgc ttcttcgatg caaggtcggc caacgtttcg
     1561 gcgccgttcg agtgaacggc aagctcaaca tctgctccga gctcaccaag gccgtggtgg
     1621 ctccgctccg aacctcgatc ggaaaccgga tcgctgtgct ctcgcaagcc gaggacgaag
     1681 aaggcgactc cattgcctat gcctggacag ccgacagtgg cagcttcgaa gactcctctg
     1741 cagtggacac gttctacacc tgtgaagaga caggcgacca ttcacttacg gtttccgtga
     1801 gcgacgacgg cttcgcctat tgcgtcgata gttggaccgt cgcgatccgc tgtgtcgacg
     1861 agggcggaac tggcggcacc ggcggaaatg gcagcggcgg catgggcggc agcggtgcaa
     1921 cgggcggctc cggcggcgca ggcggcgcag gtgcaacggg cggtgcaggc ggcacgggtg
     1981 gcgcaggtgc aacgggcggt tctggtggcg caggcggtac cggcgcaagc ggcggctcgg
     2041 gtggtgcagg tgggacgggc gcgacaggtg gcacgggcgg ctccggtggc gcaggcggca
     2101 ccggcggtgg catctgcgaa atcacgattt cggtcaccga ctcctgacgg cgacaacttg
     2161 ctgcgtacta gacctggcta cggatcgctc ggcgcatcct caggctcttc cggctcgccc
     2221 tccgcaatgt ggagctccac tcgtctgttc tgcgccagct ccgttctcgt cctcgcgttg
     2281 ggcacgagcg gacgcgtttc cccgaagccc tctgcgacga ggcggtcctg gctgactccg
     2341 cccttttcga ccaggtagcg gaccaccgag tcgacgcgtc gttgcgaaag gcgcaggttg
     2401 tagccatccg aaccggtcga gtcggtgtgg ccctcgaccc gtatgagaga aatctcggga
     2461 tgggcgttga gcacttcggc cacgttgtcg agaaccggat acgagcgcct ctgaagattg
     2521 gcgctgtcca cgttgaagta gatcttgtcg aggatctcga gctggctctc ttcgatgacg
     2581 acgtgttgct cctcggggca gccctggttc tcgactgggc caggctcgtc cgggcagttg
     2641 tcaattcggt cgggaacccc atcgccgtcc cggtcggtat cgggacagcc gtcgttttca
     2701 ggaggtccgg gctcgttcgg acacgcatcg tcgagatcga ggatcccgtc cgcatcgttg
     2761 tcgagatccg gacagccgtc ctcatcctga aagcggtcga aatcctccgc ttcgtctggg
     2821 cactcgtcgt cctcatcgac gataccgtcg ccgtccgtat cgactggcgt ttctttggct
     2881 ttccccggca tcgtataagc gagcatgccg atcaggcgcc aatcgggatt gccataaccc
     2941 gacgtcactc cacccgtgcc tgccatgccg agggtgacgc ctttctgatt caggtacttg
     3001 aagccgccga ggagctcgac cggcgtttcc tgccggccgc cgaaaccggt cgccgtgtct
     3061 gcggcagttc ggccgaacgc ctcgaccgtg atcatgaagc gttcctgtcc gagcagaacc
     3121 tggaccgctg cgccgaaggt gagctggttc ccgatctcga gcgatggaag gtcctgttga
     3181 aagctggttt tgtatccgac gttcagggga attcggaagt agtcgccggc gttgaaagtt
     3241 ccaagcaatt cgaaccatcc tccaatgtgg gggctcgcgt tgggctcgcc gaggtagttt
     3301 tgctggggtc tcgatgcaga cgccgtgttg gccgtcaaga tcgcctgcgc cgccagttga
     3361 aagacgttct cgcgcgttcc gtacagcacg ccgcgagcac ccacgtacag atcaccaaga
     3421 ccgctccctc tcgggatcag cggaccctgg ccgatcgagt cgaggaaatc gaccgccgac
     3481 tgcgagaggt tgtcgttgag catgaacgag tacgggagat ccatgaagat caccaggcga
     3541 tcccagaggc cgaggttcca cgtgaggtga ccggtgagct gtgagtgcac gagcccaagg
     3601 cgctgatcgg ggatcggacc gttggtcacc ttcagctcga gagggtcccg cgcgaaatcg
     3661 aagtagagct gcacgccgaa gcgcagatga ccctgaccgt cggcgtcgct caccgcgaaa
     3721 ccatcggtgg caagctgcga ggggcgaaac tggttgagat cgaactgagg cggagtttgt
     3781 gcgtacgcac tcgacgagaa aacgagccct gcggtgacgc cgagcaatgc cacggccaag
     3841 cgccagcgga acatcgttcg gcactctctc acccctacct cccgagctca tcggagcacg
     3901 cgcatgctca caggatgaat cagacctttg ctaccatttt tccaagtgag tggatagcgc
     3961 cgtcattcga ttggtcgttg gcctcaactc tccgtgggca ccgcggcaac cgcccatggg
     4021 agcgtcaaac gggctcgcga cttcgacgcc cgctcggcaa gcgcatgcgc caattcccag
     4081 cctcgcccga aaggactcgg ggcagccgcc atctggttca tctcgcttac cagatccccg
     4141 agcaacgcat cgagtgcttc gccgtcgagc cggagggtag cccttgctct gcgtcccttt
     4201 cttcgcggat ccaaatatcc cgaatcgccg tggcttcgcc gttgccgttc ttcgcgcgtg
     4261 agcagggtcg ccgagtgtat ggcctgcacc ggagatgcct gcggcacgat tgccgaaaag
     4321 ggcgttgggc tattgatgtc gacgacgcag tcgaagtgtt cggtccagca atggtcctcg
     4381 ccatagcttg tgtccgacat ggccagcact ttgactccgg gagtgaaaat ccgcggatcg
     4441 atctcgcgca ggcgttcgat ctttgccctc ccgctattga tgcgatccac cgtggagtag
     4501 gtcgctccat cggcgccgag cttttcgacc gccacatcga gcatggcttt cggtgacgca
     4561 ctgctgagaa tgattcggat gcctggagcg cgtgcgcgca cccaatccaa gtcttcgcgc
     4621 gagatcactt gatcaggaga ctgtcggtcc cagacccgcc gtgcgagcct tcgcaagacg
     4681 gactcgtcca ccgcctcgag gtgcgccagc gcgaccatct gaggtcgtct ctgcactgcg
     4741 ctcgtcggat gcgaaccgaa gcgagagtaa gcgagccgcc gaagccagga gatgtgcgac
     4801 gccaacttgc cccaaatcag gtagtgaatc ccggccatgg cccagcgctt ggcccctgcg
     4861 cctaggtagc gcacgagtag gatcggtctc ctcgcgtcga gcaacacgcg cccaccaaac
     4921 cagcggtgaa gctgacgtcc gtcctccggc tcgccataga cctcgtaggc gcagagctcc
     4981 caagcgagca gctcgccgag gttgcgtccg agatgggtgg tcttgtcgag gtccagaatc
     5041 aggtagtcgt gctgctcatc gagcactcga gcgcagagct cacgaaagga gatgggcgta
     5101 ccgtgatcga cgaaacggtc gacgactcgc atccgcgcag accggtcagt catgggccag
     5161 cctttcgagt acgcggctca ctccgaaacc gaggtcgagc tcccagggct gatatgagag
     5221 cttcgccgag gccgaagaca gcggcacaag tgattagata cattgcgttt tcatccatga
     5281 agttacgata tagtaactta ctataacgta acctacggcg ccgtagcatc aatgtcaaat
     5341 ggcctcgaga aaaaaccaac ggctgcggtg agaagagcac agctcatcca ggtgggaagg
     5401 cgggtcttcg ccgaaaagcc atacgagagg acctcggtac atccgagatt ccccgcgcac
     5461 gatcagaatt tcaccgtcgc catcaactgc acgaagatag gccagcctgg cgtgaagtgg
     5521 atgtcttcgc agccgacgaa agtcccatcc ggctcgctga tcggccgtgt tccgctcggg
     5581 caatcctctg ggccgtcctc acctggcagt cggccgacag tcgcgaactg agcctggcgc
     5641 catcgggtgt tgatcaagtt gagcacctgg gccttcaccg cgaatcgacg gttctcccat
     5701 ccgaatgatg cgtccactcg aacgaagcca tcggccgtta gaaaacgatc ctcggttgcc
     5761 ggccggtcgg cgatgtagaa gatgccgatg cgccccgaca gcccggtgcg tgtgtcgcgc
     5821 gcgctgaggc caccgctgat caacagctct ggcgccaagg cgacggcgtt ggcgttgccc
     5881 gggttgttca cgaactcggc ccgcacccag gtggcatcca gatcggctag gagccatggg
     5941 agaatctcga gtcggaactg tgcctccagc ccgaatcgct gcgtttcgcc acttgcttca
     6001 gtcgtgcctt catcgcctat ccacacgacc tcgctgtcga ggttgagcca gaagaacgcc
     6061 gtcgacatct gaaatcgctc ccacgcgagg actcgcgcgc caagctccca gccaagcgca
     6121 gccgaaagcg gagtggcggg gtccgtgccg aggactaccc cgcgcgcatc gttcgagtgg
     6181 aagcctcgtc cgaaattgaa gaagagctcg agcggctcta ccggatgcac gatcaggctg
     6241 gccttgggca agacgatcgc ctgtgcctcc tcgccactgc tcatcgtgcc cagggtgtcc
     6301 ctatcctcca ggcgatccgt gacgtcaaag ccgaagtact cgaagcggag gccaccgatc
     6361 aggcgcagcc acttccaagc gagctcttct tctccgaaca ggccaaggcc cccttcgctg
     6421 atgtctgcat cgacctgagt ctcgaggatc tcccgggcgg gcgcagtttg gagcgtgttg
     6481 tcgatcgcat caaagcggaa gctcgccccc atacgcgatt tcagcgtcac cctgtcgaag
     6541 cgatgggcga agccatagct cgcgcgcaag ccgaacgcgg tgcgatcgtc cccttgacgg
     6601 atcatgtcgc cgttcaccgg gtcattggcg aagaacgtga agttggaata gagggcgaac
     6661 cgcgagaggg tcacccagcc ggtgaggttg aggccttcgc ctccaatccc gcgcttcccc
     6721 tgagctccgg gtttggccgg cgactggacg ctgaggtaga ggctgtatcg ttggctctgc
     6781 cctccttcgt tcttgtcgat gcttccgaac cagtccaggt cgcccgattc cactgcgcgt
     6841 agggggatct gtcccgatgc gttccaatcg ccgagatagg ccatccccgt gagagtgagc
     6901 cgggtctcgc cacggagctc gaaagtcgtc cgagcgaaga tgttgacgcg cagaaggtcc
     6961 tcgccgtttt cgaagggccc gtctgttcca aacacctccg cggcaatgag cggcttcacc
     7021 ttgtccaact tggggctgat gatgccaaca ccgcgaaacg tccggtacgt tccgccgccc
     7081 acggtgagcg agctcatcgg cttttcatcg tcgagcacca ggttgatcgc tccggcggtg
     7141 gcaaaatccc catactcggc ctggtacgga ccttttcgaa cctccacacg ttgaatcagc
     7201 tcggggatga tgaagttgag gtcggtgtag ccctggccat gtccatggct cacccagttg
     7261 agcggcacgc cgtcgaggaa aaacgccacg tccgtaccgt ggtccgcgtc gaatccccgc
     7321 aggaagtact gattcgcttt tccaccgccc gcgtgctgaa tcacatagaa gcctggcgcc
     7381 gcattgagca gctctccggt cgagacgtac ggacgaaggc ggatttcatc gccgctgata
     7441 tcgatcgaag acgctgaaat gtcggacgga gccacgaccg ccgttgcgct gaagctaggc
     7501 tcgcgtgggc tctctccctc cggttttgcg cgttcgccgg ctgcgctctc attgactgct
     7561 ccgcgcgcat gctccggtgg gtcgaaaagc acttcgaatt gcacgattgc gggaaccggg
     7621 tcgccgtcgc gcatcgcggg ctcgaacgtg agctcgcgga cgtattgaag tgcgagcccg
     7681 gcaaactggt cggcttgctg gtcctctcgg tcgacggtga gcacgttcgc atcagccacg
     7741 cgaccctctg aatcgatcgt aattcgcagg agcactcggg ccggcttacc gtccacgccc
     7801 gccgggtagg gcggatgcat gtgcccgttg gcgggaagcg acggcgccgt cagcactgga
     7861 gcctcgccgt cgggttcgag gtcctcagcg cgcacggagg catgcatggc gatgcacgcc
     7921 accagcgctg cgatcgtggt cgggagattg cgcatgacga attgccggag gcgccgggct
     7981 cgacgaaccc ggcgactcga gttgaatgat tgagactggt cggtttgcgg ctagaccgtc
     8041 tgcgcaggcg gaccacgagc cttcgtcgtt cgtggttgcc ggtcggcggg tctctcctca
     8101 tgcgctcgga ttgcgggagc actccaggtg aggagcgcct cctgaacgac agggatcgaa
     8161 gccagcggga tctgagcggc taggtcgcga tgcgcaacgc tgccttcacc atgctcggat
     8221 ggggtttcct tttccttctc ggggtcaccc tcatgcctat gaccatgctg cgaatcatgg
     8281 tgatggccgt gatcgtgggc atcgagcgag tcgtgaaaaa cctcatgggc cagcggaacg
     8341 acgtcgaagc ctaggatcca gaggccacag gcgtcgtacg cccaatgatg cctcttccag
     8401 cctcgtgaga cggtcccgca cggcatgcgg tcgaaagata catgttgccc ccaaatctgt
     8461 cactggtccg caaacatccc ggcagtcctc agacctccga gatgccgcag acccatgact
     8521 agatttccac gattcgagcg gtagccgaac gcgggcttgc gagcgttagt ggctcggggg
     8581 gagaagcacg ccgtcaatca cgtgcgcaat cccggttcct gctcggaggt tcgcatcgat
     8641 gacgcgttcg cggttcacga acacctttcc gaagaaccac cagacataga ctgtcgaccc
     8701 ttcgagcgtc tcgacgcgcc gttctcggat caggtccttc gcgctgtttt ccccagcgac
     8761 cacgtggtag agcaagatct gctcgagcgt aaccagcccc ggctcggcga gc
//
LOCUS       DASRZF010000249        19218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_868974, whole genome shotgun sequence.
ACCESSION   DASRZF010000249 DASRZF010000000
VERSION     DASRZF010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 19218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19218
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868974"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="VFG22_10790"
     CDS             <1..645
                     /locus_tag="VFG22_10790"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET6416771.1"
                     /translation="MSDRQLRDECVTLFLAGHETTSIALGNTLYLLSKHPEIEARVHE
                     EASSVLGSRRASSDDFARLDLTERVIKESMRLYPPVYTIGRQLLSDFELGGYTIPKGH
                     MLLFVQWVTQRSDKCFVDPLRFDPDRWLPERAKAIHKYAYFPFGGGPRICIGNHFAMM
                     EAVLVLSTLIREYRFDLLPGQSLELQPAITLRAAKDLNMRLVSRHQPRGELSAR"
     gene            complement(642..836)
                     /locus_tag="VFG22_10795"
     CDS             complement(642..836)
                     /locus_tag="VFG22_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416772.1"
                     /translation="MKVEQDARFREQREQFRLKWNCEDCVLFDPEAGCAHGFPTHRHR
                     KSRYDDPAAALLFCKDFDLT"
     gene            complement(826..1269)
                     /gene="rpiB"
                     /locus_tag="VFG22_10800"
     CDS             complement(826..1269)
                     /gene="rpiB"
                     /locus_tag="VFG22_10800"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HET6416773.1"
                     /translation="MAQKLLVGSDHAGLDLKRELLGVAAELGYEVVDLGTHTSDSTDY
                     PDYAHQVSSAVARGEGLGLLVCGTGLGMSMAANRHRGVRAALCGDMFSASMARKHNDA
                     NVICIGARVVGPGLAAEILKAFLSAEFEGGRHQRRVQKIDLSNEG"
     gene            complement(1272..2510)
                     /gene="fabF"
                     /locus_tag="VFG22_10805"
     CDS             complement(1272..2510)
                     /gene="fabF"
                     /locus_tag="VFG22_10805"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HET6416774.1"
                     /translation="MRRVVITGVGTVSPCGADVATTWKNVSEGRSGIARIESFDATEF
                     ASQIAGECSDFEPLKYMPKRRLREAARFIQLAIAATAQAIEISGFEPNDEQKERVGTF
                     IGVGFCGLEVFEDTCRTLFDQGPRRVSPYFIPSVISNLAPGQVTIEWGFKGPSYTHTS
                     ACASGAHAIGDAFRWIQRGEIDAAVAGGSEAAVTPIGVAGFTSMRALSRRNDEPELAS
                     RPFDADRDGFVIAEGAGVLILEERDAAISRGAEIIAEIVGYGATSDAHHMVQPAPEAE
                     GAQRAMKLAIADAKLNPEDIDYINAHGTSTPQGDVGELQAIQKVFGAHALDGLAVSST
                     KSMTGHLLGAAGGLEPLLCTLAIRDGLLPPTINLDKPDPAGAGMNLVPNEAQERPIRA
                     LMNNSFGFGGTNCALVLKAP"
     gene            complement(2544..2777)
                     /gene="acpP"
                     /locus_tag="VFG22_10810"
     CDS             complement(2544..2777)
                     /gene="acpP"
                     /locus_tag="VFG22_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET6416775.1"
                     /translation="MDIAEKVKEIVSDKLDVDGADIQEDQDFIEDLKADSLAIVEVVL
                     ALEEQFEIEIPDEDTEKIKTVRDAIEYIKAHKG"
     gene            complement(2801..3547)
                     /locus_tag="VFG22_10815"
     CDS             complement(2801..3547)
                     /locus_tag="VFG22_10815"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HET6416776.1"
                     /translation="MFDLAGKIAIVTGGSRGIGRATSIALAEAGALVLVNYRSNEEAA
                     SETLRLLKEAGGQGELLRFDVSDPDSVEAAIKGAVTRHARIDILVNNAGISIDQLLLR
                     VSVKDLETTWATNVNGALFCAKACIRPMMKNRWGRIINVSSVVAESGNAGQVVYSASK
                     AALLGVTRTLAREYASRGITVNAIAPGFIETDMTADLPDAARQAIVDQTPLGRIGSPQ
                     EVAAAVVFLASDEASYITGQVVRVNGGMHV"
     gene            complement(3551..3745)
                     /gene="rpmF"
                     /locus_tag="VFG22_10820"
     CDS             complement(3551..3745)
                     /gene="rpmF"
                     /locus_tag="VFG22_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003154457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HET6416777.1"
                     /translation="MAVPKRRTSSMKRDQRRANHDKITAPNVSACPNCGDVMLPHRAC
                     PACGFYKGRQVTKGKAEELE"
     gene            complement(3750..4301)
                     /locus_tag="VFG22_10825"
     CDS             complement(3750..4301)
                     /locus_tag="VFG22_10825"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HET6416778.1"
                     /translation="MSAFVLQIQDLDEAGRDWNFAIGTDWLASALAETELSPGDKPGR
                     LTVHAQRNGSDVLVQGHVEAELATTCARCLADVPLEVDLALTALFSPEQTRPRGSEDI
                     DVQLDEVNRDYYSGAEVVLDSMVREYFLLEAPMKTLCSESCEGIAIPEHVRPPDEVFG
                     DSAPDSRFAPLLKLKEALTKNEE"
     gene            4610..5470
                     /locus_tag="VFG22_10830"
     CDS             4610..5470
                     /locus_tag="VFG22_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET6416779.1"
                     /translation="MITLRAMADDFQRFSGTSTYLTSDALESAVNCAVVLERPLLIRG
                     EPGTGKTLLAEAVSESLRMPLLRWNIRSTTRAQDGLYVYDTVQRLYDSRFGDKDVNDI
                     SQYIKLGPMGEAFNAEERVVLLIDEIDKADVEFPNDLLNELDRMQFHVDETQQDIIAT
                     QRPIVIITSNAEKELPDAFLRRCVFHFIDFPTPELMNDIVKVHHPKVHESLVDQALKT
                     FYEIRGMRRLRKRPSTSELVDWIAVLQRAGIDSVKLEENLPFLGALLKKEQDLVAFAD
                     QLSGGRQWRS"
     gene            5487..6659
                     /locus_tag="VFG22_10835"
     CDS             5487..6659
                     /locus_tag="VFG22_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6416780.1"
                     /translation="MYELRGRGVPVGTQEVIALAQALKAGLHESSLDGFYNVARSVMI
                     HSEAHLDSFDEAFLSFFKGIEIESKKLKDELWDWLQQAKENIGELTDEQREFVENLDL
                     DELRKLFEERLAEQDEQHDGGNKWIGTGGTSPFGHSGRAPKGFRIGGSGGGRTAVKVA
                     DARLYRPYRQDLTLDVRQMQVALRKLRLFSREGGEEELDLEGTIDATAQNAGELEVVT
                     RPPRRHNTRIILMMDVGGSMDPFSHLCSRLFSAAKKSSHFKELRTYYFHNCVYGHVYE
                     TERFDTPIKVRDLIHETGPHYKLIMVGDALMAPYELLAAGGSLDLGDDRGIEGLQWLM
                     MLSQHYHRSIWLNPEPQRYWTGNTIEYVRQTFDMFPLTLEGLGEGVAHLLKGGKGH"
     gene            6729..7211
                     /locus_tag="VFG22_10840"
     CDS             6729..7211
                     /locus_tag="VFG22_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416781.1"
                     /translation="MGKTSIIAVLVIALGSLAATASANESSYAGVNPGSETSENLPPK
                     AEEIPEGALMLTWPGFMMLEDGSCFFVQTSKPVKFGTQKSEGRLELVLHDTRVHLKNN
                     FLPLETQFFDTPVTRATVQHRGKKDVAMVFEMREDATPTITQSKGKDGFNYIFVKFPP
                     "
     gene            7323..8582
                     /gene="hisS"
                     /locus_tag="VFG22_10845"
     CDS             7323..8582
                     /gene="hisS"
                     /locus_tag="VFG22_10845"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HET6416782.1"
                     /translation="MSLRAVKGMKDILPDEISNWHRLENAFRQRVEAYGFGEARFPIV
                     EPTALFVRSIGETTDIVEKEMYTFADRSEKSLTLRPEGTASSVRAYVQHSVQAREPVT
                     KWYYIGPMYRRERPAKGRYRQFYQAGIEVFGDPGPYIDAEVIDMVVGFISELGITEVE
                     VLVNSIGGPETRENYREALLGYLAPFRGELCSDCQRRMDANPLRVLDCKVPRCSEIAS
                     DAPSILKHLTDDDRAHFDGLQETLTLLGTPYRVDGSLVRGLDYYTRTLFEVLGKGEGL
                     GAQNALLGGGRYDQMVESLGGPDTPAIGFAMGLERLLMAMPAAESDSRVDVFVVAAQA
                     GVRAQATLLGRELRNDGLRVESDLRGGSLKSQLRRADKLNARIAMILGDAEVEAGVVQ
                     LKDLERKTQDEVPRAEAGARARSLLSG"
     gene            complement(8592..10346)
                     /locus_tag="VFG22_10850"
     CDS             complement(8592..10346)
                     /locus_tag="VFG22_10850"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HET6416783.1"
                     /translation="MRFSLGGDGSIHGEPGATDHSDAIMCWAPGTYQPINDPRAELKA
                     EHQGWRVEVQHEGPRSTVLVAPPSGGEFVVWTAFGIAAAPCIAAAQGGAWVGFHHDVR
                     EDTGVPDIAKWIALRFVDMEGRIFEPAAPMTDRNRDLDGEEQGFEFPALVVGADGAVD
                     LFGRGSHQMYRQRLNADGFSPREPIDDGQWGCRGRHLTAVRLESDTTLAAWRGRKGIE
                     IRAFDVPSGGSPQLQAAKVSVGEFDAAPIHLTAWSGPQVLFGDIQQHSAHSDGVGSAD
                     EAYLRARYRYGDDFAALTDHESFLGKRIGPGEWAYLQAVTERHDEPGEFATIVAYEWT
                     GKSYPGPGHKCIYLPGPGFPIISRDEVPEGRDIVAQIREQGAFTGPHHIGWTGADVPG
                     HDPEGQPVWEICSCHGCYEHPDHPLGYRGELYDQFAKPLLDSGMRFGFIANSDSHGLL
                     WHHGECRKRDPFRTGLTAVISTSRTREAILAAIRARRCYATSGAKILLAVHVAGFPMG
                     SELDIEGPVEVHARVLGTTDIERIELMTPAGVAASEAPKSPSASFVGEVDAPYVYCRV
                     TQADGEMAWSSPIFFNRA"
     gene            complement(10470..12725)
                     /locus_tag="VFG22_10855"
     CDS             complement(10470..12725)
                     /locus_tag="VFG22_10855"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HET6416784.1"
                     /translation="MRFFLLLVMTLAVALGCSRGAGKPDPFAGLKAHTDLTALRHLAE
                     VEDGGWYIDFGTPAQGKYTLGDWRSGWLGKGVDGDTSYANVGMRGRVYFNSDRSEPLV
                     VRIRLRPHGTHALTPYLNNKQLASIHLGKGEGFAEYELTLPAEEVQPGENQLLLTFGG
                     TTPVDGQDVSVSIDSIWIRNASEAAPTAPLAREPAYDTLVANVRLGDEERQAIALSRM
                     STLRYYVAVPKSGSLGFGIGVEGEAGAPFTIEVTADGQPAVEVFTGTASTSWTDHKVD
                     LSQFAGETVRLDLKAKDPGAGRIAWSSPSIYVPTAEERNIEPAKNVIVLVIDTLRADK
                     LRPFNPATRVKTPAIDRFAAEGAVFELAQSPENWTKPAVASILTGLHPQTHQQKTGDA
                     ALPGSAELLSEHLKDAGFATGSFIANGYVSDRFGFDQGWDDYSNYIREQKSTEAKDVF
                     EQAGNWIEAHKDGRFFAYIQTIDPHVPYDPPGQYLEMYDPSEYSGQIRPRMTGDLLEK
                     AKRRPPQVVFTESDKRRLKALHDGEISKHDHFFGEFLERLSALGLSDDTLIVVTADHG
                     EEFEDHGSWGHGHSVYQELLHVPLLFRLPNRIPAGARVNDAVSTLDVSSTVTELLAVP
                     AMTQNEGHPLVGLMLGEASSHPTVAFSDFQDDRRVITTGRWKLVIRGNLTSTMFDLRA
                     DPGEKTPLDSTAFPIGRRYSRMMLGQFLGATDRADWLSAEQKSGTQLQRENAEMDDTI
                     RDQLRALGYAH"
     gene            complement(12809..14713)
                     /locus_tag="VFG22_10860"
     CDS             complement(12809..14713)
                     /locus_tag="VFG22_10860"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6416785.1"
                     /translation="MSVAPEDAACRGALFEQLADLRRSAAELGLPHLETALAEALARL
                     EKEAFGPAALAGMRVLAWRYEALAAMPGESGTHPVVRERGSVEPSLRGRRVLVADDEA
                     EVRWSYVAVLREASARVIEASDGAHALALAREHAPDVILADIVMPRLDGLGLCAAIRR
                     EPLLDGVPVVLLSWRDDFLHRMQELRVEAQGYLRKETPAREILQRVSRVLAPLADLEA
                     DLRSDRDVIGELGDLGVSSLLRAVRRLRPSANIVIQDPWSLFDLVLHEGKLVAVTRTA
                     IDGAITRGAPALPALVGMSSGRFVVAKATVSEASRERASLDGDFESASRHLAAILNRM
                     AEHPDCRIEFDEDVLGTCARHSPFRVQGLIERLAAGEAPEAVRASGAASRTLMDALLV
                     TLARQGAIRDVTVAASTEIEPDSVPVEDAVERENVRAQSAVAMHRDPANRASPWIDPI
                     WRLSASASAASAEGSSDFEAEVQTRPRLFGLAYLVALAVTVAFLIWNQTLATPSETAQ
                     EGPAMARPEPVAPPAPRPPPSPERSSSSSARGLYAFSGALRGGVDPSLAVADGQGVLE
                     LSGSRDVRIEVDGVERGPLPISVVLAEGTHAVRYRNESGSTYRFYFVKSGATRSLQVV
                     TRPGGLVDPR"
     gene            14800..15906
                     /gene="obgE"
                     /locus_tag="VFG22_10865"
     CDS             14800..15906
                     /gene="obgE"
                     /locus_tag="VFG22_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HET6416786.1"
                     /translation="MKPYALAQCTHPADLFEHQVGCSGKAADVVSCVDFVDEVTLDVA
                     AGDGGNGCVAFRREKYVPLGGPSGGDGGRGGSVILMAHERLSTLLDLRFRKIIRAERG
                     EDGRGKDQFGKAGSDIRVQVPVGTQVYEQDSGALLADLSESGAELVVANGGRGGRGNM
                     KFATPYDRAPRRADPGQPGERKKIRLVLKLLADVGIVGFPNVGKSTFIATVSRAHPKV
                     ADYPFTTLVPNLGVVSLGLDRTFVIADIPGIIEGASEGAGLGLRFLKHVERTKVLLHM
                     LSVDPDPDRDPVTDFDTLMAELERFDPRLLERPMVVAVSKCDLPEARDAYEDVRRSLE
                     PRGFQVFAISSATNDGLNVLLEALEAILRRPDDA"
     gene            complement(15878..16939)
                     /gene="queG"
                     /locus_tag="VFG22_10870"
     CDS             complement(15878..16939)
                     /gene="queG"
                     /locus_tag="VFG22_10870"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HET6416787.1"
                     /translation="MNRADDQLTARVKAEAEAAGFARTGIAEAGELGEEGAQLDAWLA
                     AGCHGQMTWMADTAPVRKDPRNPKMLAQARSVIVMAAPYAQAYGYEGPGPARIAKYAH
                     GRDYHNVLSKKSRKVARLLQDEGFAARIAVDSKPVFERAWAERAGLGFVGKNCCLIVP
                     GIGSHVFLACVVTTAPLNADAPIERRCGSCTLCLEACPTKAFSEPRRLDARRCISYLT
                     IEHRGPIPIEHRKALGPWAFGCDVCQDVCPYNQASGATSQSLDAFAPGSRWMDFDLTS
                     LLQMSEGEFRDWSEGSPLKRARREGLARNAALVLGNRGDARHLPILESASRNHPDEVV
                     REAANWARSELRRRLAASE"
     gene            complement(16936..18537)
                     /locus_tag="VFG22_10875"
     CDS             complement(16936..18537)
                     /locus_tag="VFG22_10875"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB C-terminal domain-containing protein"
                     /protein_id="HET6416788.1"
                     /translation="MRRRYREPVVIAFALALIASLFVHLPVYEVLGSLADRFRTEAEE
                     AKKERKFDPVEVDFEVPSDSKSQRARKKQRQREEVQREKIAKVVQEQQQQEQQARQNQ
                     PPPPPQVEAPQAVQQHSTNPDVEPPENAKFIADQNNRVEEESVARIRNMVRDDKLPDP
                     GKQEKPSNTLEEQGNSKEQKIAEARNKEGSDARKVTEEEARRERPDKAIKADPIKAER
                     VASQAPNGKQAATSGDRAGEAADDTITITDAAGTFSIRRPSTASQGGGQRTEGRGPGG
                     QMAWSQLEGAVGADELEQDRRAYLAEHKSGQQGISREKTWKEFRAAIENYVPGVKPGA
                     TTALNAAASPFATYIAAVHRRIHQEYADRFLRSLPMGSPYQDPTLVTKLEIVLNRDGS
                     VYRVGIVHSSGLLPFDFGAFNSVLRAQPYPPAPSEILSGDGRVYFHWGFYRNERQCGT
                     FNAEPYILPNPRGTPAREKGLTDAPDWETVVPADAKPTWKTRDEESKDPDEPKRPPKK
                     SPPGQPKEKEEAPSSPPVPPGAGLG"
     gene            18723..19043
                     /locus_tag="VFG22_10880"
     CDS             18723..19043
                     /locus_tag="VFG22_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416789.1"
                     /translation="MLDRPPAEPFQEPPPERPTVRPRAEPLELGRPTEPARAALFPKR
                     PTVRVGEGVVAADPRVEALPVRDGFARGWRTSEASGRERGTVLVSVGLSCGRAALCTR
                     AALG"
BASE COUNT         3465 a         6329 c         5856 g         3568 t
ORIGIN      
        1 atgagcgaca ggcagttgcg cgatgaatgc gtcacgctgt ttctagccgg gcacgaaacc
       61 acctcgattg ccctaggcaa cacgctctat ctcctctcga agcacccgga gatcgaagct
      121 cgcgtccacg aagaagcgag ctctgtcctc gggagccgaa gggccagctc cgacgacttc
      181 gcgcgcctcg atctcaccga gcgggtcatc aaggaatcga tgcgccttta ccccccggtc
      241 tacacgatcg ggcggcagct cctctcggat ttcgagctgg gcggctacac gatcccaaag
      301 ggacacatgc ttctgttcgt ccagtgggtc acgcagcgga gcgacaagtg ctttgtcgat
      361 ccgctccgat tcgaccccga tcgctggctc ccagagcgcg ccaaggcgat tcacaagtac
      421 gcgtacttcc cgtttggcgg gggaccgcgc atctgcattg gtaaccactt cgcgatgatg
      481 gaggcggtcc tcgtcctctc gaccttgatc cgcgagtacc gtttcgactt gctgcccggg
      541 cagtctctcg agctccagcc cgcgatcacc cttcgcgccg ccaaggactt gaacatgcgc
      601 ctcgtgtcac ggcatcagcc tcgaggcgag ctatcggcac gctaagtgag atcgaagtcc
      661 ttgcagaaca ggagcgcggc ggccggatcg tcgtaccgtg acttccgatg acggtgcgtc
      721 ggaaacccat gcgcgcaacc tgcctctgga tcgaacaaga cacagtcctc gcagttccac
      781 ttgagccgaa attgctcgcg ctgctcgcgg aatcgcgcgt cctgttcaac cttcattcga
      841 gaggtcgatc ttctggacgc gccgttggtg ccggccgcct tcgaactccg cgctcaggaa
      901 cgctttgaga atctctgctg ccagccccgg acccacgaca cgcgcgccga tgcagatcac
      961 gttggcgtcg ttgtgctttc gggccatcga tgcgctgaac atgtcgccgc aaagagccgc
     1021 acgcacgccc cggtgacggt tggcagccat ggacatgcct aggcccgttc cacaaacgag
     1081 aagaccgagc ccttctccgc gggcaacggc gctcgacacc tggtgcgcat agtcggggta
     1141 gtcggtcgaa tcgctcgtgt gcgtgccgag gtcgaccact tcgtagccga gctccgcggc
     1201 aactccgagc agctcgcgct tgagatcgag ccccgcatga tccgaaccga ctaagagttt
     1261 ctgagccatg actacggggc ctttagcacg agcgcgcaat tggtcccgcc aaagccgaac
     1321 gagttgttca tcagggcgcg aatcggccgc tcctgcgcct cgttgggtac caggttcatc
     1381 cccgctccgg ccggatcggg tttgtcgaga ttgatcgtcg ggggaagaag accgtcacgg
     1441 atggcaagcg tgcagagaag cggctcgagc ccgccggcag cgccaagcaa gtgaccagtc
     1501 atgctcttgg tgctggatac ggcgaggcca tcgagggcat gggcgccgaa gactttctga
     1561 atggcctgca gctcgcccac atcaccttgc ggtgtggagg tcccgtgcgc gttgatgtag
     1621 tcgatgtcct ccgggttcaa cttggcatcc gcaatcgcaa gcttcatcgc gcgctgcgct
     1681 ccttcggctt cgggcgccgg ttgaaccatg tggtgcgcgt cggaggtcgc gccgtagccc
     1741 actatctcgg cgatgatctc cgcgcctcgc gaaatcgctg catcccgctc ttcgaggatc
     1801 aacacgccag cgccctcggc gatcacgaag ccgtctcgat ccgcatcgaa ggggcgactc
     1861 gcgagctccg gctcgtcgtt ccgccgcgac agtgcgcgca tcgaggtaaa cccggctaca
     1921 ccgatcgggg taaccgcagc ttcgctccca cctgccacgg ccgcgtcgat ctctccgcgt
     1981 tgaatccagc gaaaggcgtc accgatcgcg tgcgcgccag aggcgcaggc gctggtgtgt
     2041 gtgtagctcg gccccttgaa cccccactcg atcgtcacct gcccgggcgc taggttcgag
     2101 atgaccgagg gaatgaaata gggcgatacg cgtcgcggtc cctggtcgaa gagcgtcctg
     2161 cacgtatcct cgaagacttc gagcccgcaa aagccgacgc cgatgaacgt gcccactcgc
     2221 tccttctgtt cgtcgttcgg ttcgaagcca gagatttcga tcgcttgcgc ggtagccgcg
     2281 atggcgagtt ggatgaagcg cgcggcttcg cgcagccgtc gcttcggcat gtacttcaat
     2341 ggctcgaagt ccgagcactc tcctgcgatc tgtgaagcaa actccgttgc gtcgaagctt
     2401 tcgatgcggg cgatgccgct ccgaccttcg gaaacgttct tccaagtggt ggcgacatcc
     2461 gcgccgcagg ggctcaccgt gcccacgcct gtaatgacta ctcgccgcat cgacctaccc
     2521 ctggacgcgt tcgcgcgggg ctctcagccc ttgtgcgctt tgatgtactc gatggcgtcg
     2581 cggacggtct tgatcttctc cgtatcctcg tccgggatct ctatctcgaa ctgctcttcg
     2641 agcgccagga cgacttcgac gatcgcgagt gagtcggcct tgagatcttc gatgaagtct
     2701 tgatcttcct gaatgtcggc cccgtccacg tcgagcttgt ccgacacgat ttctttgacc
     2761 ttctcagcga tgtccatctg cttcctccgt cacgcgcgtc tcagacgtgc atgcctccat
     2821 tcactcggac tacctgcccg gtgatatagc tcgcttcgtc gcttgctaaa aacactactg
     2881 cggccgcgac ctcttggggg ctgcctattc gaccgagggg cgtctggtcg acgatggcct
     2941 gcctggccgc atccggcaga tctgcagtca tatcggtctc gatgaaccct ggggcaatgg
     3001 cattcacggt gattcccctg gatgcatatt cgcgggccaa ggtcctcgta acacctaaaa
     3061 gcgccgcctt cgaggccgaa tagaccacct gcccggcgtt gccggactcc gccacgacgc
     3121 tcgaaacatt gatgattcgg ccccagcggt tcttcatcat cggtcgaatg caggccttcg
     3181 cgcaaaacaa ggctccgttg acgttggtgg cccatgtggt ctcgagatcc ttcaccgata
     3241 ctctcaacaa gagctgatcg atggagattc ctgcattgtt gaccaggatg tcgattcgcg
     3301 cgtggcgcgt gacggcccct ttgatggccg cttcgaccga atccggatcg gatacgtcga
     3361 accggaggag ctcgccctgc cccccggcct cttttagaag ccgcagcgtt tcactcgcag
     3421 cctcctcgtt cgagcgatag ttgaccagga ccagcgcacc ggcctcggcc aatgcgatgc
     3481 tcgtcgcacg cccgatgcct cgcgaccccc cagtcacgat ggcgattttc cccgccagat
     3541 cgaacacgag ctactcgagc tcttcggctt tgcccttggt cacttgtcga cctttgtaga
     3601 agccgcaggc tgggcatgcc cgatgcggaa gcatgacatc gccgcagttg gggcatgcgc
     3661 tcacgttcgg ggcggtgatt ttgtcgtgat tggctcgacg ttggtcgcgc ttcatcgagc
     3721 tggttcgtcg tttcggtact gccacgggat cattcctcgt tcttcgtaag tgcttctttg
     3781 agtttgagta atggggcgaa gcgcgaatca ggcgccgaat ccccaaagac ctcgtcggga
     3841 ggtctaacgt gctccggaat cgcgattccc tcgcagctct cggagcaaag tgtcttcatc
     3901 ggcgcctcga gcaggaagta ctctcggacc atggagtcca agaccacctc ggcgccgctg
     3961 tagtagtctc ggttgacttc gtccaactga acgtcgatat cttcgcttcc tcggggacgg
     4021 gtctgctccg ggctgaagag cgcggtgagg gccaaatcca cctcgagcgg gacgtcggcc
     4081 aggcagcgcg cgcaggtcgt ggcaagctca gcttcgacat gaccctgcac caagacatcg
     4141 ctgccgttgc gctgggcgtg cacagtcagc cgcccgggct tgtcgccggg gctaagctcg
     4201 gtctccgcga gggcggacgc cagccaatcc gtgccgatgg cgaagttcca atccctcccg
     4261 gcctcgtcga ggtcctggat ctgtaggacg aatgcagaca ttgagttgag ctcgataaac
     4321 tccagccaag cgaccctccc aggtcacaag aacgcgctac tattcgccct gccctcagag
     4381 ctgtcaagga gggtgcgcgc gccccggccc accgcctaaa ccctctgtaa tattgagtac
     4441 tttcgaccgg atggcgtcag ccggcactgt cgctgtggct ggcactggca ctgtcgccgg
     4501 cactgtcgct ggcactggca ctggcgctga cactggcact gccgtcgatc ctgcacgcct
     4561 gcaggagatc cctgcccacc ccctctcccg gtcgccaatt cggaggagcg tgataacttt
     4621 gcgcgccatg gcagatgatt tccaacgttt ctcgggtaca tcgacttatc tcaccagtga
     4681 tgcgctggaa tccgccgtca actgcgcggt tgtgctcgag cggccccttt tgatccgggg
     4741 cgagccaggc accggcaaga ccttgctggc cgaggcggtg tccgagtcgc tccgaatgcc
     4801 gcttcttcgc tggaacatcc gcagcaccac acgcgcccaa gatgggcttt acgtctacga
     4861 caccgtccag cggctttacg actcccgctt cggggacaaa gacgtcaacg acatcagtca
     4921 gtacatcaag ctcggcccga tgggcgaggc gttcaacgcc gaggagcgcg tcgtcttgct
     4981 gatcgacgag attgacaagg cggacgtgga gttcccgaac gatctcttga acgagctgga
     5041 ccgcatgcag tttcacgtcg acgaaacgca gcaagacatc atcgcgaccc agcgccccat
     5101 cgtcatcatc accagcaacg ccgagaagga gcttcccgac gcgttccttc gccgttgcgt
     5161 gttccacttc atcgacttcc cgacgccgga gctcatgaac gacatcgtca aagtgcatca
     5221 tcccaaggtc cacgagagcc tcgtcgacca ggcgctaaag acgttctacg agatccgagg
     5281 catgcgacgg ctccgcaaac gcccgagcac cagcgagctc gtcgattgga tcgccgtgct
     5341 ccaacgggcg ggcatcgaca gcgtgaagct cgaagagaat cttcccttcc tgggcgccct
     5401 gctcaagaag gagcaagacc tcgtcgcttt cgcagatcaa ctgtcgggcg gccggcaatg
     5461 gagaagctga aatactagtc cctttcatgt acgagctgcg cggccgaggc gtcccagtgg
     5521 gaacgcagga ggtgattgcg ctcgctcaag ccctgaaggc agggctccat gaaagctcac
     5581 tcgacgggtt ctacaacgtc gcccggtcgg tgatgatcca cagtgaggcg cacctcgact
     5641 ccttcgatga agcgtttctc tcgttcttca agggcatcga aatcgagtcg aagaagctca
     5701 aagacgagct ctgggactgg ctgcagcaag ccaaggagaa catcggcgag ctcaccgacg
     5761 agcagcgaga gttcgtcgag aacctcgatc tcgacgagtt gcgcaagctc ttcgaggaac
     5821 gtcttgccga gcaggacgag cagcacgacg ggggtaacaa gtggatcggc accgggggca
     5881 cctcgccttt cggccacagc ggccgcgcgc ccaagggctt tcggatcggc gggtcgggcg
     5941 gcggaagaac cgccgtcaag gtcgcggacg cgcgcctgta tcgcccctac cggcaggacc
     6001 tcacgctcga cgtgcgccag atgcaggttg cgcttcgtaa gctgcgcctc ttctcgcgcg
     6061 aaggtggcga ggaggagctc gacctcgaag gtacgatcga cgccacggct cagaacgccg
     6121 gcgagctcga agtggtgact cgccctcccc gtcggcacaa cacgcgcatc atcttgatga
     6181 tggacgtggg agggtcgatg gatccgttct cgcatctttg ctcccggctc ttttccgccg
     6241 caaagaaatc gtctcacttc aaagagctgc gaacctatta ctttcacaac tgcgtctacg
     6301 gccacgtcta cgagacagag cgcttcgaca cgccgatcaa ggtgcgcgac ctcatccacg
     6361 agacgggtcc gcactacaag ctgatcatgg tcggcgacgc gctgatggcg ccctacgagc
     6421 tcctcgccgc gggtggctcg ctcgacctcg gcgacgaccg gggcatcgaa ggccttcaat
     6481 ggctgatgat gctcagccag cactaccacc gaagcatctg gctcaacccg gagccccagc
     6541 gctactggac cggaaacacg atcgaatacg tgcgacagac cttcgacatg tttccgctca
     6601 cgctcgaggg cctcggcgag ggtgtcgcgc acctgctcaa gggcggcaag ggccactgaa
     6661 gcggcccgaa aacccgtcga gacgagctaa atttgggcag gcgcccccgg gtcgcttact
     6721 ctgaggtcgt ggggaagaca tccatcatcg cggtgctggt catcgcattg ggaagcttgg
     6781 ccgcgaccgc ttcggcgaac gagtcctcgt atgccggagt gaacccgggg tcggaaacct
     6841 cggagaacct cccgccgaag gccgaagaga tcccggaggg cgcgctcatg ctcacctggc
     6901 cggggttcat gatgctcgaa gacggatcct gcttcttcgt ccaaacgtcc aaacccgtga
     6961 agttcggaac gcagaagtcc gagggccgac tcgagctggt tcttcacgac acgcgggttc
     7021 acctcaagaa caacttcctg cccctcgaga ctcagttctt cgacaccccg gtgacgcgcg
     7081 cgaccgtgca gcaccgaggc aagaaggacg tcgcgatggt cttcgaaatg cgcgaggacg
     7141 caacgccgac catcacgcag agcaagggca aggacggctt caactacatc ttcgtcaagt
     7201 tccccccgta aaggccacgc tgcgcgttgc gcacggggct tctgccctca aaacccaggc
     7261 ggcacatggc ccatgaggcg tcgccccttg cttgccgtgg ttccatcgag taaccggacg
     7321 ccatgagtct gcgcgcggtc aaggggatga aagacatcct acccgatgag atctcgaact
     7381 ggcatcgcct cgaaaacgcg ttccggcagc gcgtcgaagc ctacggtttc ggcgaggcgc
     7441 gctttccaat cgtcgagccg accgcgctct tcgtccgttc gattggcgaa acgacggaca
     7501 tcgtcgaaaa agagatgtac acattcgccg accgaagcga aaagtcgctc acgcttcgcc
     7561 ccgaaggcac ggcatcgtcg gtacgcgcct acgtgcaaca cagcgtgcaa gcacgggaac
     7621 ccgtaacgaa gtggtactat attgggccga tgtatcggcg agagcggcct gccaaaggac
     7681 ggtatcgcca gttttatcag gccggaatcg aggtcttcgg cgatccaggc ccctacatcg
     7741 acgccgaggt gatcgacatg gtggtcgggt tcatctccga gctcggaatc accgaagtcg
     7801 aggtcttggt gaattcgatc ggcggtcctg aaacccgcga gaactatcgc gaagctttgc
     7861 tcggctacct cgcaccgttt cgcggcgagc tatgcagcga ctgtcagcgg cgaatggatg
     7921 ccaatccact tcgcgtgctc gactgcaagg ttcctcgatg ctcggagatc gcttccgatg
     7981 caccgtcgat cctcaagcac ctcaccgacg acgaccgcgc acacttcgat ggactccaag
     8041 agactctcac cttgctcggc actccttatc gcgtcgacgg ctcgctcgtc cgcggtctcg
     8101 actactacac tcgaaccctg ttcgaagtgc tcggcaaggg tgaggggctt ggcgcgcaga
     8161 atgccttgct cggcggcgga cgatacgacc aaatggtcga gagcctcggc ggcccggaca
     8221 ctccggcgat cggcttcgcc atgggactcg agcgcctcct gatggccatg ccggcggccg
     8281 aatccgattc ccgcgtcgat gtgttcgtcg tcgccgcaca agccggcgtc cgtgcccaag
     8341 ccaccctcct cggccgcgag cttcgaaacg acggcctccg ggtggagagt gatcttcgcg
     8401 gcggctctct caagagccag ctgcgccgtg ccgacaagct caacgcacgc atcgccatga
     8461 tcctcggtga cgccgaggtc gaagcaggcg tggttcagct gaaagatctc gagcgaaaga
     8521 cgcaggacga agtcccgagg gccgaggccg gagcgcgcgc ccggagcctg ctctccggct
     8581 gagctcgaac cctaggcgcg gttgaagaag atcggactcg accaggccat ctccccgtcg
     8641 gcctgagtca ccctgcagta cacatacgga gcgtcgacct cgccgacgaa cgaggccgag
     8701 ggcgacttcg gcgcctccga tgccgcgacg ccggcaggcg tcatgagctc gatgcgttcg
     8761 atatcggtgg taccgagcac tcgggcatgc acttcgaccg gcccttcgat atcgagctcg
     8821 cttcccatcg ggaaaccagc cacgtgtacc gcgagaagaa tctttgcgcc gctggtcgcg
     8881 taacagcggc gcgcccgaat cgccgcaaga atcgcttcgc gggttcgact ggtagagatc
     8941 accgccgtga gccccgttcg aaatggatcg cgtttgcggc actcgccatg gtgccaaaga
     9001 agaccatgac tgtcggagtt cgcgatgaag ccgaagcgca tcccgctgtc gagtagcggc
     9061 ttggcgaact ggtcgtagag ctcgccgcga tatccgagtg ggtggtcggg atgctcgtag
     9121 catccgtggc aggagcaaat ctcccaaacg ggttgcccct cgggatcgtg ccccggtacg
     9181 tcggcaccgg tccagccgat gtgatgcgga cccgtgaaag ctccctgttc gcgaatctga
     9241 gcaacgatgt cgcgcccttc cgggacctcg tctcgagaaa ttatcggaaa cccggggcct
     9301 ggaaggtaga tgcacttgtg gcctggaccc ggatacgatt tacccgtcca ctcataggcc
     9361 acgatggtcg caaactcgcc aggctcgtcg tgacgctcgg tcaccgcttg aaggtatgcc
     9421 cactcgcccg ggccgatgcg ctttcccaag aaagactcgt ggtctgtgag agccgcgaaa
     9481 tcatcaccat agcggtagcg cgcgcgaagg tacgcctcgt ctgcgctgcc gaccccgtcc
     9541 gaatgagcgc tgtgctgctg gatgtctcca aacaaaacct gaggcccgga ccaggcggtc
     9601 agatgtatcg gcgccgcgtc gaactcgcct acgctcacct ttgcagcctg gagctgcggg
     9661 ctacccccac tcggtacatc gaacgctctg atctcgatgc cctttcgacc tctccaagcc
     9721 gccaaggtcg tgtcgctctc gagacggacg gcagtgagat gcctgcctcg gcatccccat
     9781 tggccatcgt cgatcggttc cctgggtgag aagccgtccg cattgaggcg ttgccggtac
     9841 atctggtgcg agccgcgccc aaagagatcg accgcaccat ccgctccgac gaccaacgcg
     9901 gggaactcga agccctgttc ctcaccatcc agatcccgat tgcgatccgt catcggtgct
     9961 gcgggctcga agatccttcc ctccatgtcg acgaaacgaa gcgcaatcca ctttgctatg
    10021 tcgggcactc cggtgtcctc acgaacgtcg tggtgaaaac cgacccaggc cccaccctgt
    10081 gcggcagcga tacatggggc agccgcgata ccgaaagcgg tccagacgac gaactcgccc
    10141 ccagagggcg gcgccaccag tacggtcgac cgcggcccct cgtgttgcac ctcgacccgc
    10201 cagccctggt gctcggcttt gagctctgcg cgcggatcgt tgatcggttg atacgtgccg
    10261 ggcgcccagc acatgatcgc gtcgctgtgg tcggtcgcac ccggctcccc atggatcgag
    10321 ccatcgccgc cgaggctgaa ccgcatcacg cactcttctt caccggacca cgcgagaaca
    10381 tgcaggagcg cacctccgtt ggccgctggc tcaagccgcg ggtttcgaag gtaccgctcg
    10441 cgagtctgtt cgattcgcgg catcaggcct cagtgtgcgt agccaagcgc gcgcagctgg
    10501 tctcgaatcg tgtcgtccat ctcggcgttc tcccgctgaa gctgtgtacc gctcttctgc
    10561 tccgcactca accagtccgc ccggtcggtg gcgccgagaa actggccgag catcatgcgg
    10621 gagtaccgcc tgccaatcgg aaaggccgtc gaatcgagcg gcgtcttttc cccggggtcc
    10681 gcgcgcaggt cgaacatcgt ggaagtgaga ttgccacgga tgaccagctt ccagcggccg
    10741 gtggtgatca cgcgccggtc gtcttggaag tcactgaacg ccaccgtcgg atgcgaggac
    10801 gcctccccga gcatcaaacc gaccagcggg tggccttcgt tctgcgtcat cgcgggaacg
    10861 gcgagcaact cggtcaccgt ggaggagacg tcgagtgtgc tgacggcgtc gtttaccctc
    10921 gctccagccg gaatgcgatt gggcagccga aacagaagag gcacgtgcag aagctcctgg
    10981 tagaccgagt ggccgtgacc ccaggacccg tggtcctcga attcttcgcc gtggtccgcg
    11041 gtcaccacga tcagggtgtc gtcgctcaac ccgagggcgc tcagccgttc gaggaactca
    11101 ccgaagaagt gatcatgctt cgagatctct ccgtcgtgaa gggccttcaa gcgtcgcttg
    11161 tcactctcgg tgaacacgac ttgcggcggc cgtcgcttgg ccttctccag cagatctccg
    11221 gtcatgcgcg gccggatctg tcccgagtac tcgctcgggt catacatctc gagatactgc
    11281 ccgggcgggt cgtacggaac atgcggatcg atcgtctgga tgtacgcgaa aaagcggccg
    11341 tccttgtgcg cctcgatcca gttcccggct tgttcgaaca catccttggc ttcggtactc
    11401 ttctgttcac gaatgtagtt cgagtagtcg tcccagccct gatcgaagcc aaagcggtcc
    11461 gatacgtagc cattggcgat gaagcttccg gtcgcgaaac cagcgtcctt caggtgctcg
    11521 ctcagaagct cggccgagcc gggaagcgct gcatcgccgg tcttttgctg gtgcgtctgc
    11581 gggtgcagcc ccgtgagaat cgaggccact gcgggtttcg tccagttctc gggcgactgc
    11641 gcaagctcga acaccgcgcc ctcggcggca aagcggtcga tggcaggcgt ctttacacgg
    11701 gtggccggat tgaaggggcg cagcttgtcg gcgcgaagcg tgtcgatgac gagcacgatg
    11761 acgttcttcg cgggctcgat gttccgctct tccgcggtcg ggacgtaaat gctcggcgag
    11821 ctccaagcga tgcgacccgc tcccgggtcc ttggccttca gatcgagacg cacggtctcg
    11881 ccggcaaact ggctgaggtc gaccttgtga tcggtccagc tcgtggatgc cgttcccgtg
    11941 aacacctcga cggcgggctg tccatcggcg gtgacctcga tcgtgaacgg ggcacccgcc
    12001 tcgccttcta cgccaatccc gaatccaagc gacccgctct tcggaaccgc gacgtagtag
    12061 cgaagggtcg acatcctcga aagcgcgatg gcctgccgct cctcgtcacc aagacggacg
    12121 ttggcgacca gtgtgtcgta cgcgggctct cgcgccagag gtgccgtcgg tgcggcctcg
    12181 gacgcgttcc gaatccagat ggaatcgatc gaaaccgaga catcttgccc atcgaccggc
    12241 gtagtgccgc caaaggtcag caagagctga ttctcaccgg gctgaacttc ctccgcggga
    12301 agcgtgagct cgtactcggc aaaaccctcg cccttgccga gatgaatcga ggcgagctgt
    12361 ttgttgttta gatacggagt cagcgcatgt gtcccgtgcg ggcggagccg aatgcgaacg
    12421 acgagcggtt cggaacgatc gctgttgaag taaacgcgac cgcgcattcc gacgttcgcg
    12481 tagctcgtat cgccgtcgac gcctttgccc agccagccgc tacgccagtc gccgagcgtg
    12541 tacttcccct gagcaggcgt gccaaaatcg atgtaccagc caccgtcttc cacctcggcc
    12601 aagtggcgaa gcgccgtcag atcggtgtgc gccttgagcc ccgcaaacgg gtcgggtttg
    12661 cccgcccctc gactgcaacc cagcgccacg gcaagggtca tcaccagcaa caaaaagaat
    12721 cgcatcagcc gccttcctcc cgcgctccga cggccgagtg cgcccttctt tcggcctgtg
    12781 ggaacctcgc tgcggttagc atgcagcttc agcgagggtc aactaaacca cccggccggg
    12841 tgaccacctg cagcgagcgc gttgcacccg atttcacgaa gtagaagcga taggtggatc
    12901 cagactcgtt gcgataacga acggcgtgcg ttccctcggc aagcaccacg ctgatcggca
    12961 gggggccacg ctcgacgcca tcgacctcga tcctcacgtc gcgcgaaccg ctcagctcca
    13021 ataccccttg cccatcggca accgccaagg acggatccac gcccccacgg agagctcccg
    13081 agaaagcata gagtccgcgg gccgacgatg aagaggaacg ttcgggcgac ggcggaggcc
    13141 ttggggcagg cggcgccacg ggctcgggtc tcgccatcgc cgggccctcc tgcgccgtct
    13201 cagagggggt ggccagggtc tgattccaga tgagaaacgc gacagtgacg gcgagcgcga
    13261 ccaaatacgc caagccgaat aagcgtggcc tcgtctgtac ttccgcctcg aaatcagagc
    13321 tgccttcagc gcttgccgcg ctcgcgctgg cgctcaaacg ccaaatcgga tcgatccacg
    13381 gtgacgcccg attcgctggg tcccggtgca tggcaaccgc cgattgtgct ctcacgttct
    13441 ctcgttcgac ggcgtcctcc acgggcaccg agtctggctc gatctcggtc gaagccgcaa
    13501 cggtcacatc gcgaattgcg ccttgccgcg ccagagtcac cagcagcgcg tccatcagcg
    13561 tccgagatgc agcgcccgat gcccggaccg cctccggtgc ctcgccggct gcgaggcgct
    13621 cgatgaggcc ctgaacccga aacggcgaat gacgcgcaca ggtcccgagc acgtcctcgt
    13681 cgaattcgat ccggcaatcg gggtgctcag ccatccgatt cagaatcgcc gcaagatgcc
    13741 gggatgcact ctcgaagtcg ccatcgagcg acgctcgctc gcgtgacgct tcgctcacgg
    13801 tcgctttcgc gacgacgaat cgccctgagc tcattccgac cagcgcgggc agggccggcg
    13861 cgccacgggt gatcgcacca tcaatggcgg tgcgcgtgac cgcgacgagc ttgccctcgt
    13921 ggagcaccag atcgaacaag ctccaagggt cctgaatgac gatgttcgcg cttgggcgaa
    13981 ggcgtcgaac cgcgcgcaag agcgacgaga cgccgagatc tccgagctcg ccgatcacgt
    14041 cgcgatcgga gcgcagatcg gcctcgaggt ccgccaaggg cgccagcaca cgcgacactc
    14101 tttgcagaat ctcgcgtgct ggcgtctcct tgcgaagata gccctgggcc tccacgcgga
    14161 gctcttgcat ccgatgaagg aagtcatcgc gccatgacag cagcaccacg ggcacgccat
    14221 cgaggagcgg ctcgcggcga atggcagcgc acagacccag tccgtcgagc cgaggcatga
    14281 cgatatcggc gagaatgaca tcgggagcgt gctcgcgggc gagcgcgaga gcatgcgctc
    14341 catcgctcgc ttcgatcacg cgcgcgctcg cctcccggag caccgcgaca taggaccagc
    14401 gaacctccgc ttcgtcgtca gccaccaaaa ctctccggcc ccgaagactc ggttcgacac
    14461 tgccgcgctc tcgcacgacc ggatgggtcc cggactctcc cggcatcgcc gcaagagctt
    14521 catagcgcca tgccaagaca cgcatgccag cgagcgccgc aggcccgaac gcctcctttt
    14581 cgagtcgagc cagggcttcg gcaagtgcgg tttcgaggtg cgggaggccc agctcggcgg
    14641 ccgatcgacg aagatcggcg agttgttcga acaaggcgcc gcggcatgca gcatcctcgg
    14701 gcgcaacgct caatgcctcc gttaaatgta ttgtttccaa gctcttatcc gccaaagctt
    14761 caacgaactt ggcgtttcca gtcgcgagct cggagtctcg tgaagcccta cgcacttgcg
    14821 cagtgtacgc atccggcgga tttgttcgag caccaagtcg gttgctccgg caaagctgcc
    14881 gacgtagtga gctgcgtgga cttcgtcgat gaggtgacgc tcgacgtcgc agcgggggac
    14941 ggtggcaacg gctgcgttgc gtttcggcgc gagaagtacg tgcccctggg cggcccctcg
    15001 ggcggcgacg gcggacgggg tggaagcgtc attctcatgg cgcacgaacg gctctcgacc
    15061 ttgctcgact tgcgcttccg gaagatcatc cgcgccgagc gaggcgaaga cgggcgcgga
    15121 aaagatcagt tcggcaaggc cggctccgac atccgggttc aagtccccgt tggcacccag
    15181 gtatacgagc aggacagcgg cgccctgctc gcggatctca gcgaatcggg agccgagctc
    15241 gtggtcgcaa atggcggccg aggtggccgc ggaaacatga aattcgcgac gccgtacgac
    15301 cgcgcgcccc ggcgcgccga cccgggacag cccggtgagc gcaagaagat ccgcctcgtt
    15361 ctgaagctcc tcgccgacgt cggcatagtc ggtttcccca acgtcggaaa gagcaccttc
    15421 attgcgaccg tgtcccgagc tcaccccaag gtcgccgact acccgttcac caccctcgtc
    15481 cccaacctcg gcgtcgtctc actcggcctg gaccggacct tcgtcatcgc cgacatcccc
    15541 gggatcatcg agggcgcctc cgagggagct gggctaggcc tgcgttttct gaagcacgtg
    15601 gagcgaacca aggtgctcct ccacatgttg agcgtcgacc cggatcccga tcgagacccg
    15661 gtgaccgact tcgatacgct gatggccgag ctcgaacggt tcgacccccg cctcttggaa
    15721 cggccgatgg tcgtggcagt gagcaagtgc gacctccccg aggctcgcga cgcgtatgaa
    15781 gatgtgcgac ggtcgctgga accccgaggt ttccaggtct tcgcgatcag ctccgccacc
    15841 aacgatggcc tcaacgtctt gctcgaagcg ctcgaggcta ttctgaggcg gccagacgac
    15901 gcctgagctc gcttcgtgcc cagttcgcag cctcgcgaac gacctcgtct ggatggttcc
    15961 gcgatgccga ctccaaaatc gggaggtgtc gcgcatcgcc gcgattcccc aacacgagcg
    16021 cagcgttacg agccaaacct tcgcgccgcg cccgtttcaa gggggagcct tccgaccaat
    16081 cgcggaactc cccttcgctc atctgcagca gcgaagtcag atcgaaatcc atccaacggc
    16141 tgccgggcgc gaaagcgtct agcgactggc tcgtcgcgcc cgaggcctgg ttgtacggac
    16201 agacgtcctg gcacacgtca caaccaaagg cccaagggcc aagcgccttc cgatgctcga
    16261 tcggaatggg gccacggtgc tcgatcgtga gataggaaat gcaccggcga gcgtcgagcc
    16321 tgcgtggctc ggagaacgcc ttggtcggac aggcttcgag acatagggtg cagcttccgc
    16381 atcgacgctc gatgggcgca tcggcattca gcggcgctgt ggtgaccacg caggctagga
    16441 agacatgcga accgatcccg ggaacgatca ggcagcaatt cttaccgacg aagcctaggc
    16501 ccgctcgctc ggcccaggcc cgctcgaaga cgggcttcga atccacggca atgcgcgccg
    16561 caaacccctc gtcctgaagc aggcgcgcca cctttcgact cttcttgctg agtacgttgt
    16621 ggtagtcgcg cccgtgcgca tatttcgcga tccgcgcagg tcccggcccc tcgtatccgt
    16681 acgcctgcgc gtagggcgcg gccatcacga tcacgcttcg cgcctgcgca agcatcttgg
    16741 gattccgcgg atcctttcgc accggcgcag tatccgccat ccaggtcatc tgcccatgac
    16801 acccagccgc cagccatgcg tcgagctgtg cgccttcctc tccgagctcg cccgcctcag
    16861 caatgccggt ccgcgcgaac cctgcggctt cggcctccgc cttgacccgt gcggtgagct
    16921 gatcgtcggc gcggttcatc ccaggccggc gccgggtggc accgggggcg aagacggcgc
    16981 ttcctctttt tccttcggct gtccgggcgg agacttcttc ggaggtcgct tgggctcgtc
    17041 cgggtccttg gactcctcgt cgcgggtctt ccaggtcggc ttcgcatcgg cgggaacgac
    17101 ggtctcccaa tcgggggcgt cggtcagacc tttctcacgc gcgggtgtgc cgcgcgggtt
    17161 gggaaggatg taaggctcgg cgttgaacgt accgcattga cgctcgtttc gatagaagcc
    17221 ccagtggaaa tacacccgcc catcgccgct cagaatctca gagggcgccg gcggataagg
    17281 ttgcgcacga agcaccgagt tgaacgcacc gaagtcgaac ggcagaaggc cactcgaatg
    17341 cacgatgccg acccggtaga ccgacccgtc gcgattgagc acgatctcga gcttggtcac
    17401 cagcgtcggg tcctgatagg ggcttcccat cgggagactc ctcaagaaac ggtcggcgta
    17461 ttcctgatgg attcgccgat gcaccgctgc gatgtatgtc gcgaagggtg aggctgcagc
    17521 attgagcgca gtggtcgcac caggcttgac cccgggcacg tagttctcga tcgccgcgcg
    17581 aaactccttc caagtctttt cacgcgagat gccctgctga ccggacttgt gctcggcaag
    17641 gtatgcgcga cggtcctgct cgagctcgtc ggcgcccacc gcgccttcca gctgggacca
    17701 ggccatctgc ccgcccggcc cgcggccctc tgtacgctga ccaccacctt ggctagcggt
    17761 gcttggccga cggatgctga acgtccccgc cgcatcggtg atcgtgatgg tgtcgtccgc
    17821 agcctccccg gcgcgatcgc cggaggtggc ggcctgcttt ccattcggcg cttggctggc
    17881 gacgcgctca gccttgatgg gatcggcctt gatcgctttg tcgggtcgct cccggcgggc
    17941 ctcctcttcg gtgacctttc gagcgtcgct gccttccttg ttgcgcgcct cggcaatctt
    18001 ttgttctttg gagttgccct gctcctcgag cgtgttggac ggcttctctt gctttcctgg
    18061 atcgggaagc ttgtcgtcgc gaaccatgtt tcgaatccgg gcgacggatt cctcctcgac
    18121 ccggttgttt tgatccgcga tgaacttcgc gttctcaggg ggctccacgt cgggattggt
    18181 gctgtgctgc tgcaccgcct gaggcgcctc gacctgcggc ggaggaggtg gctgattctg
    18241 cctcgcctgc tgttcctgtt gctgctgttc ctggacgacc ttcgcgatct tctcccgctg
    18301 gacttcctcg cgctggcgct gtttcttgcg cgcgcgttgc gatttcgagt cgctcggaac
    18361 ttcgaagtcg acctcgaccg gatcgaactt cctctccttc ttcgcctctt cggcctcggt
    18421 ccgaaatcga tcggcaaggc ttccgagcac ctcgtagacc ggaaggtgca cgaagagcga
    18481 tgcgatcaac gcaagcgcga aggcaatgac gactggttct cgataacgtc ggcgcacgga
    18541 aggtcccttg ggggtaagat ggacttcccc tctttagagt tgcaccagcc aggcgacagg
    18601 gcaaagagtc gattagcggc tagcgacccc gcgcggtgga gacgcttcgg cccccgccaa
    18661 agccccctgc cgagggcctg ccgaagcttg cgccccggcc aaagctcgag cggcttcctc
    18721 ccatgctcga tcgccccccg gccgagccat tccaggagcc gcctcccgaa cgcccgacgg
    18781 tacggccgcg tgcggaaccg ctcgagctgg ggcggcccac cgagccggcg cgtgcagcgc
    18841 tattccccaa gcggccgact gtgcgcgtcg gagaaggcgt cgtcgcggca gacccccggg
    18901 tggaagcact tcccgtgcgc gacgggttcg cccggggctg gcgcacgagc gaggcgtctg
    18961 ggcgtgagcg cggtactgtg ctcgtcagcg tcgggttatc ctgcggtcgt gccgcgcttt
    19021 gcacgcgagc tgcgctcggc tgagatctgc tgaccgaggc aacgcgaccg ctcggcgcac
    19081 gcgtcacggt cgacgttcgc atgctcggcg ctcgcgtcac cgtggaagtc cgagtgctcg
    19141 gcgcgcgcgt cacggtcgac gttcgcatgc tcggtgctcg cgtcacggtc gacgttcgca
    19201 tgctcggtgc tcgcgtca
//
LOCUS       DASRZF010000250        38869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_523651, whole genome shotgun sequence.
ACCESSION   DASRZF010000250 DASRZF010000000
VERSION     DASRZF010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 38869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38869
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523651"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="VFG22_10885"
     CDS             complement(<1..997)
                     /locus_tag="VFG22_10885"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET6416790.1"
                     /translation="MTLLDGTLRRRHWIAMGLGLLGLAAAGVWVVRQRNIDHESGADP
                     HGQHAQQGDIEYYTCPMHTSVRKHEPGKCPICGMDLTPVAKDESGSGTIIVDEDRRQQ
                     IGVKTSEVERRDIIRQIRAVGRITIDETRLADVNLRMSGWVHRLLVAETGQKVKEGQT
                     LFTLYSPELYAAQREYLAARAQKPGGDASAISELSQASEQRLRLLGMSQGQVRELERR
                     GHAWENVPIASPSTGYVIDKDIVEGGRIEAGTRVYRIADLSRVWVDAEVYESDLAQLE
                     PGQAVKVDLPYGPGRELEGQVDYIYPTLSGATRTARVRVVLANPDLALKPDMYADV"
     gene            complement(984..2372)
                     /locus_tag="VFG22_10890"
     CDS             complement(984..2372)
                     /locus_tag="VFG22_10890"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET6416791.1"
                     /translation="MNRIPIATAIVAWLVGSLGMSGCAHGESARYDAQRSALESARQE
                     GLPNQRQTVEPAFLEEGAPLQREALVRAVLARNPSLEAARQAWRAALAEYPQVRSLED
                     TMASYVIAPLSIRSDTVRFGQVVRLEQRFPAPGKFRFAGEVALAEAEAAQGGHDAMRL
                     ALALMASSLFDEYYRVTRVLELNDQHRQLIEDIKATAMIQYEAGRASQQDVLQSEVEL
                     AHIIHQRVMLESEREVIVAQINGLLHQRPELPLPEPPAMIAPASAEIADTSALQDEAL
                     DNRPELHAARSQLHAADSSVGLAKRNYSPEIGVIGEYNSMWAENDHRWMAGVALNLPL
                     QIRSRKGAVEQAEARAQEALAALASQHDEVRVEVDQARHRVLEAHHVVQLYRERLIPA
                     AKAQIDAAEFAYETGRSNFQALIDAERSVRSLEIEYEQALASLGQRAAELDRARGIVP
                     GIGAEGGQHDAP"
     gene            complement(2472..2816)
                     /locus_tag="VFG22_10895"
     CDS             complement(2472..2816)
                     /locus_tag="VFG22_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416792.1"
                     /translation="MTTAGVAGAALHLCGMEELVLRHCCCQDANDGPPVQLKRVDDCC
                     GALFSKGEQPPFSSSNDRLNVDAPMLSLSALATDFSRVEPAQETVWIPLARGSPGAHG
                     PPIFIWNCSYLT"
     gene            3034..5277
                     /locus_tag="VFG22_10900"
     CDS             3034..5277
                     /locus_tag="VFG22_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HET6416793.1"
                     /translation="MTDQSNPHTCPMHPEVRQERPGSCPKCGMALEPVGPAAQATHTE
                     WTCPMHPEIVRAEPGSCPICGMALEPRNVASEEEEGANPELADMTRRFWIAAAFTIPL
                     FLVAMGDMLPGAPVSRILSPSARTWLELGLATPVCLWAAWPFYVRFAASLENKSLNMF
                     TLIGLGVGVAYGYSVVAALAPGVFPEAFREGGTVAVYFEAAAMIVTLILLGQVLELRA
                     RGQTGAAIKKLLGLAAKTARRLRADGSEEDLPLDAVRVGDQLRVRPGEKVPVDGVVLE
                     GKSAVDESMVSGEPIPVEKGEGDSVIGATVNGTGMLVVRAEKVGNETLLSRIIAMVAE
                     AQRSRAPIQKLADTVSGYFVPIVIGVSVTTFVVWSLVGPEPRMAHALINAVAVLIIAC
                     PCALGLATPMSIMVAMGKGASNGVLFRDAEAIEVMRKVDTLVVDKTGTLTEGKPVLVT
                     VETVAGIDATELLGAAASLERASEHPLAEAIVAGAVERGAHLERPESFDSITGQGVRG
                     RIGDRDLALGNRKLLEGMDVDPGILAARADTLREEGQTVMFVVVDGRLSGILGVADPI
                     KESTPDAIADLHKEGIRVVMLTGDNQKTANAVAAKLSIDEVIAEVLPDEKAEVIKRLQ
                     QGGSIVAMAGDGINDAPALAQADVGVAMGTGSDVAIESAGVTLVKGDLRGIVRARKLS
                     RATMANIKQNLFFAFVYNGFGVPIAAGVAYPIFGLLLSPVIAAAAMSFSSVSVIANSL
                     RLRRLEI"
     gene            complement(5305..7284)
                     /locus_tag="VFG22_10905"
     CDS             complement(5305..7284)
                     /locus_tag="VFG22_10905"
                     /inference="COORDINATES: protein motif:HMM:NF005539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6416794.1"
                     /translation="MYLVTGASGFIGKHLLDALVRRGQTIYALVLPRSEASFWELVDK
                     RWPSARSQFVVLAGDISEPLCGLSVERIEELRDKVQHMFHLAALYDMTAGMEESERAN
                     VTGTRNACRLAEALDATLHYTSSTAVAGDYVGFFREDMFDEGQKHKNPYFKTKFLAEK
                     LVRDECQTRYKIYRPGAVVGSSIDGQADKIDGIYYAFKLIQRMRRALPSWFPLIGFEG
                     SELHVVPVDYVARAIDAIAHNEEVSSNTFHLTDPKPKSFGDALNLLCDAAHAPSFDAR
                     IDPRVLKLVPRGLVGLLGAMPAVKTARREVLADIGIPESVLPYVNWRSSFDTRETEAA
                     LARTGITCPPFETYAWKIWDYWERHMDPDLFLDRTLHGRIAGKIAMVTGASSGIGEAL
                     AIRLAEAGAKVLLVARSKEKLEAVQRTIDLRGGESLIHTCDLSSSEDVDRLVREVLDA
                     YGHVDLLVNNAGRSIRRGVAHSYDRYHDFERTMQLNYFGSLKLILGLLPTMRERKDGQ
                     IVNISSIGVQTNAPRFSAYVASKAALDAFSRSIASEVVGDGVCITTVYMPLVRTPMIA
                     PTKIYEAVPTRSTDEAVDMIVDGIVNRKKRVATRLGIFGEVSYAVAPKLIDRVLNTGF
                     RLFPDSPRKGSKDEDHKAPGPEAVAFAHIMHGIHW"
     gene            complement(7331..7912)
                     /locus_tag="VFG22_10910"
     CDS             complement(7331..7912)
                     /locus_tag="VFG22_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416795.1"
                     /translation="MLFGLAVAFCACSDPAADAEQIPLAVESQDNIVSENGQLTLGAG
                     TLSILSVSLIGADSNVVLLGPVIIDLAIPRQEPSLNSNVPAGEYAGLQIQLAPAGGRG
                     EMLDVEVSSVMTGDLVRVTSSLSMSGQVVFPEGPRTIAEGSNLELRLGLQGMFFYLSP
                     ITDAVDGVYVAGENARDFLTMDLINMFDLSVLP"
     gene            7911..8588
                     /locus_tag="VFG22_10915"
     CDS             7911..8588
                     /locus_tag="VFG22_10915"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416796.1"
                     /translation="MTLLTHREGVRIMGNSGVRNGQMHPRLREYIKGALLHTDAILAT
                     RKATLEEVAGFVATKRSEGGGAELIFICTHNSRRSQMAELWAAAAAQHFAIDGVRTFS
                     GGTEATAFDPRAVAAVERSGFLVKNTGGENPRYRITFADDGLILECFSKTYDDPFNPA
                     RGFAAIMTCSDADEACPLVLGAALRARLPYDDPKVADGTPDETLQYDARCMQIATEML
                     YLFSRVH"
     gene            complement(8585..9460)
                     /locus_tag="VFG22_10920"
     CDS             complement(8585..9460)
                     /locus_tag="VFG22_10920"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6416797.1"
                     /translation="MTKSSERVAVVTGSSRGVGKGIAIALGAGGATVYVTGRSHQGGK
                     EHPLGGTVESTAAAVTEAGGHGIAVTCDHSDDDQVHELFQRVEQEQGRLDILVNNATA
                     VPRELAKPGPFWEKSLDLLRILDVGLRSHYVASYFAAPLMVHTGGGLIVFTSSYGGRV
                     YMHGPAYGAGKAGIDKMAMDMAVDLRDHDVASVSLWLGFVRTERNEPLFQHPDAMKGP
                     YGKFLANAESPEFIGRVIEALHRDPQRMKRSGRVLIAAELAESLGVREADGGQPKSNR
                     RTLGAPPEPSPVIVR"
     gene            complement(9465..10526)
                     /gene="arsB"
                     /locus_tag="VFG22_10925"
     CDS             complement(9465..10526)
                     /gene="arsB"
                     /locus_tag="VFG22_10925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HET6416798.1"
                     /translation="MTMTKRGLSFLDRYLTLWIFVAMALGIGLGYLVPGFADALDSMS
                     VGTTSIPIAIGLILMMYPPLAKVRYEEMGRVFRNKKVLALSLVQNWLIGPILMFALAV
                     VFLSDKPEYMVGLIIIGLARCIAMVIVWNDLALGDREFAAGLVAFNSIFQILFFSVYA
                     WFFVTWLPGKLGLEGMAVDITIGEIAKSVAIYLGIPFAAGFLTRRGLIRAKGRAWYDA
                     SFAPRIGPITLVALLFTIVVMFSLKGDAIVALPFDVVRIAVPLLIYFVAMFFISFWLS
                     KVAGADYPVATSLSFTAASNNFELAIAVAVATFGIHHGAAFAAVIGPLVEVPVMIALV
                     NVALRIRTRYFSPAAVLEA"
     gene            10580..11335
                     /locus_tag="VFG22_10930"
     CDS             10580..11335
                     /locus_tag="VFG22_10930"
                     /inference="COORDINATES: protein motif:HMM:NF018066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HET6416799.1"
                     /translation="MKAALTEPRPSKYQIQGRTVTLPCVVRDACSATVTWLVDSRAAQ
                     SLLPGPDLQIAEVAPGRGLLSIACIDYIDNDLGDYNEVSIALFVRKRGDRRGLPYLGS
                     IVDMMRGLLPTHIIHLPVNQSFTCEAGRQIWGFPKTVDEIDFDTSGERARCIWDRDGQ
                     NVLKLSAPIGGNRKFPEQTLSTYSYIDGALHETRFASSAESLGIRLGGAELELGAHPV
                     ADELRTLGLPKPALMSMWMGKMTGRFEAAQRCT"
     gene            11355..12464
                     /locus_tag="VFG22_10935"
     CDS             11355..12464
                     /locus_tag="VFG22_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HET6416800.1"
                     /translation="MEVRAAVAHEAGKPLTVETVQLEGPRAGEVLIEIKATGVCHTDA
                     YTLSGADPEGIFPSVLGHEGAGVVVEVGAGVQSVKPGDHVIPLYVPECRNCEYCTSGK
                     TNLCQAVRATQGRGLMPDASSRFSIGNETIFHYMGTSTFAQYTVVPEIAVAKIREDAP
                     FDKVCYIGCGVTTGIGAVINTAKVEPGANVVVFGLGGIGLNVVQGARMVGADKIIGVD
                     INPARRELAEKFGMTHFVNPTEVDGDLVAYLVDLTGGGADYSFECIGRVDVMRQALEC
                     CHKGWGESVIVGVAGAGEEISTRPFQLVTGRVWRGTAFGGARGRTDVPKIVDWYMEGK
                     INIDDLITHKMPLEEINTAFDLMHQGKSIRSVVEL"
     gene            12466..13149
                     /locus_tag="VFG22_10940"
     CDS             12466..13149
                     /locus_tag="VFG22_10940"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6416801.1"
                     /translation="MGSESAVFVHQFELGPWDNFIYFIGDKATRKCAVVDPAWDSDFI
                     LEEAQRLDVEISNILCTHSHFDHVNRVDALLEKRDVPVHMLGEEIDFSGFHCENLKRQ
                     SPGDRLRIGEQLEISMLHTPGHTPGSTTYRIRDAIVTGDTLFIQGCGRCDFVGGDPEL
                     MYRTLKTLTESLPRETRMYPGHNYGPMTVSTLDEELEANPYLQHQTVRDFVSHRMEGK
                     TPGTPLPQP"
     gene            complement(13173..13805)
                     /locus_tag="VFG22_10945"
     CDS             complement(13173..13805)
                     /locus_tag="VFG22_10945"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET6416802.1"
                     /translation="MNEVSTPGTVSATIGPPSGGLGRFDIRMNLKYIPNAICIARIIL
                     VAPIVWSLFEGRYALALGLILLAGFSDALDGYLARRFDWRTRLGGLLDPAADKLLMFA
                     AYVSLAWIGWVPVWFSAIVIGRDLVIIAGTIIYQVYVAPVHGEPTGASKLNTVLQILF
                     VLLTITHAWLGQPPMLVLRVLGAAILLTIAISTVQYVSTGVTRARAKHPH"
     gene            13880..15265
                     /locus_tag="VFG22_10950"
     CDS             13880..15265
                     /locus_tag="VFG22_10950"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF005899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET6416803.1"
                     /translation="MSAFGDDALGASDAVAIAARIQSGGLRACDAVEAAITRAERVNP
                     ALNAIVTPLFDSAREQAKSPPPGTFSGVPTFIKDSDAVAGSPFFFGSRGLPRKPAEKS
                     SAFVQQFLSLGLINLGKSSTPEFGLTGTTEPLAHGPTHNPWKLGFSTGGSSGGSAALV
                     AAGVVPIAYANDGGGSIRIPAACCGLVGLKPSRGRLIEIEGSKLLPVRLLHQGMLSRT
                     VRDTAAFYHAAERYYRNPRLPEIGLVTRPGRRLRIGFFSEFGESAPAHPECVTAVNDV
                     ATLCEGLGHSVEQVRAPFDEGFHEDFFLLWATIAFVVTRFGKQLIHPDFDRAQVEPFT
                     EGLAQYFLDNRVDFPATVWRLRRFARRYARGFEDYDVLMNPTVGMPPPEHGYIGPDVP
                     FETALERLKWFIPFSPVQNVSGGPAISLPLAQSSQGLPIGVQLASSMGTERTLLELAI
                     ELEAAAPWPSL"
     gene            complement(15354..15767)
                     /locus_tag="VFG22_10955"
     CDS             complement(15354..15767)
                     /locus_tag="VFG22_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HET6416804.1"
                     /translation="MSGATRGSCVCGAVAFRIEGPYRGFQYCHCSRCRKKTGSSHVAN
                     IFVPVTQFAWERGEENVKRFELPEAKYWSTAFCTSCGSAVPWLTRNGKAMVVGAGALD
                     DDPGVKPTKSVFFGSRAPWYVHVSDLEMFETLPDS"
     gene            complement(15764..16318)
                     /locus_tag="VFG22_10960"
     CDS             complement(15764..16318)
                     /locus_tag="VFG22_10960"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HET6416805.1"
                     /translation="MHFTTRAILVLGILMMASASPASAKEVDDEKLASDVRDAMTRFE
                     EKDSSFKKTTESVVGYAIFPEVAKGGFIVGGSGGRGEVYEGGKLIGYGKLSEGTIGAQ
                     VGGQKFSQIILFKTKSALNRFKKNRFELSAQATANAADQGGAAKAKYADGVAIIILPT
                     SGLMADASIGGQKLKFIPLADAKK"
     gene            16433..18283
                     /locus_tag="VFG22_10965"
     CDS             16433..18283
                     /locus_tag="VFG22_10965"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HET6416806.1"
                     /translation="MSESRTMPGYIAATAATSPDRHALAEMGPDGRWVFTTWGDYWQA
                     VRDLAKGLIALGLEPGDGVALIGDNRRDWVISQMGISAAAAVPAPIYVTNTVEQVAFI
                     VRNCRAKIAICDGEAQLQKYLTALDRGLIELEHIIVMDAIESEREMVVSLREVIKRGR
                     GEPDGEVERRMAEAKPDDVALLIYTSGTTGVPKGAMCTHRGVEAMAASFMDKYPELFE
                     FDPLRYVSYLPLCHVAEQIFTNFIGVRVASETFFCADLGKIKDHLVAARPHFFFAVPR
                     VWEKFEAALGANLLSATGAKAKLADWARRVELQSAKRSMATGRQVDSVRRRLANKLVI
                     SKIKSAIGLDELYAAGSGAAPASRSTLEFFASIGIIIHEGFGMTETTGMATAQPFGAP
                     HFGSVGQALPGVEIRIADDGEIMLRGPNMVPGYLYMPEETASLYAEDGWMHTGDLGVL
                     DDEGFLSITGRKKDLLITAGGKNVAPAEMENHLQGIPGVGQAIVVGDRQPYLCAILAL
                     EAEAVPELCRAAGVPEAPLAEVARNQKVRAFLADRVESECNSKVARYQTIKKFEVLPH
                     MLSVDGGELTPTMKIKRNVINEKYRALIDGMYASGGGDSSPKPGSTSPAR"
     gene            18336..19343
                     /locus_tag="VFG22_10970"
     CDS             18336..19343
                     /locus_tag="VFG22_10970"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HET6416807.1"
                     /translation="MQKLAFVFGALLALSLAVVGPAVAEDPEFIMEVGTVAPKGSPWA
                     EQLQRYKAEAQEKTGGRLKVKLRLGRGNERSLARRVQMGSMQAFAGSVGGMSSIVREV
                     NVIEAPYVFENFAAADKALDDPEVTAQVKRVLEKRGLVFGIWAENGYRSYFSKMKIRT
                     PDDMKGVRFRSQESMVHVATYEAFGATPVPIDNGNTLMSVQTGVVDGFDNTPLFAMAG
                     SWYQGLSEGERNIFLSRHCYQPAILVYSKKWFDSLPKDIQEVITTMPHDLTIWGRNQV
                     RKIEPVLLKNLERYGYDIYDPSPKELEAFKAKQKNVPDKVAKEMGPEGVALLKAIRKT
                     F"
     gene            complement(19357..20352)
                     /locus_tag="VFG22_10975"
     CDS             complement(19357..20352)
                     /locus_tag="VFG22_10975"
                     /inference="COORDINATES: protein motif:HMM:NF016817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HET6416808.1"
                     /translation="MPSAGKPKTGSKVRPRPPFEVNGVTAPAGRLTEVELRIARLPTG
                     MWFSLPVGVVHGKNPGPVIWISAAIHGDELNGVPIIRHVLDRIDPAKLSGTVLAVPAV
                     NVLGLVQESRYLPDRRDLNRCFPGSKRGSSASQLAHLFMTEIVARSSVGIDLHTGSGG
                     RTNLPQLRCDLEDPETLRLAREFAPPILVHARVRDGSLRGAAAELGKTVLLYEAGEAS
                     RFSASAIDMGVRGILRVMQALDMLDSSTEPAAPAPRISRSSSWLRAQRTGFCEMMVSL
                     GAEVREGDTVAIIFGALGRKQMAVKAKSAGVVIGHVTSPIVHRGDAVANIATVTG"
     gene            complement(20312..21214)
                     /gene="rimK"
                     /locus_tag="VFG22_10980"
     CDS             complement(20312..21214)
                     /gene="rimK"
                     /locus_tag="VFG22_10980"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HET6416809.1"
                     /translation="MRIGILSRNGKLYSTKRLKEAAIERGHEVRVVDYTRCYMNITSH
                     RPQVLLSGDALEFDAIIPRIGASNTFYGTAVVRQFEMMRTYSLNSSQAISRSRDKLRA
                     LQLLAREGIGLPVTGFARSTKDVEGLIHMVGGAPVIIKLLEGTQGMGVVLAETDKAAE
                     SVMSAFIQLDANILVQEFIKEAGGADIRAFVIGDKVVASMKRQGPEGEFRSNLHRGGS
                     AKTVKLTPEERSTAVRSASKLGLRVAGVDMLRSNHGPVVMEVNSSPGLEGIEASSGKD
                     VAGLIIEYLEKNAKRGKTKDRIQG"
     gene            complement(21211..21726)
                     /locus_tag="VFG22_10985"
     CDS             complement(21211..21726)
                     /locus_tag="VFG22_10985"
                     /inference="COORDINATES: protein motif:HMM:NF017434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK/LysX family protein"
                     /protein_id="HET6416810.1"
                     /translation="MSETKATEGRIRVRPHESTTIGWREWLALSELGIGRIKAKIDTG
                     ARSSSLHAFDIQVTGEGKHPWVHFKVHPIQRDSVTIVQCKVRLYEYRNVKSSSGHSER
                     RPIIRTPMRLGEETWPIELTLTSRDEMGFRMLLGREAIRGRFVVDPNRSYLFRELVPK
                     RHKKRRKKGPS"
     gene            complement(21754..23079)
                     /locus_tag="VFG22_10990"
     CDS             complement(21754..23079)
                     /locus_tag="VFG22_10990"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HET6416811.1"
                     /translation="MQDPTVLWKLLATAIFVVLNGFFVAAEFGLVKVRPMRIDALVRS
                     GDRRAAIVQRMLQRLDLYLSACQLGITIASLVLGWLAEPAVARLLLELAAYAGWQIHD
                     GPTTHLIAVAIALTIITILHMTVGEQAPKVWAIQKAESISLFVAYPLYTFATALRPLI
                     SFINALSNGLVRLAGMSTADEHDASASIEELRSILASSARAGHITARQRSIGDNVLAL
                     MRLEVRHIMLPRVDVAYLSTSNTMEENLRILRETGHSRYPLGDPDLDNIRGVVHARTV
                     LATLLQEKELSLEAQALRMPTVPDTQPLSRFIVELQRSQTHAAAVVDEHGTIIGLSFL
                     EDALEEIVGPIHDEFDEESSEMTEVSPGVFELAGNVPLPEASDFLAVPLDEDEADTIA
                     GYFISYLARMPKRGDTLQIGDYKATVLQVLRHRIVRLRFERVENPESAA"
     gene            complement(23135..24439)
                     /locus_tag="VFG22_10995"
     CDS             complement(23135..24439)
                     /locus_tag="VFG22_10995"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HET6416812.1"
                     /translation="MSSAVTEVVGHFLRSHPAEAARKFESASPKDVADVLIEESPQVA
                     ATVLDYVQPDHAAEVLAAAGKDRARDLIPAIDPARAANLLARLPEATRADLLGVVSAS
                     LAKEIEGILAFPSGTAGRLMDARVAVFSKDTLVSAALERIQGLRKRRISDIVLTNDEG
                     KFDGVVRLQDLVGADPEQPLAELKNPRVAFVPPMTPREDVVELLNEYQLSSLPVVDLQ
                     NRVVGIIRQAGLVEAVQEDAIMGLQQMVGGSTEERALSRPWVGVRSRLPWLNINLITA
                     FMAASVVGLFEDTIARFTAVAVLLPVVAGQSGNTGAQALAVTMRGLALREFRVSEAFR
                     VLVKELIIGLVNGLAIATVTSIGVYAWSRNLGLSLVMFLAMIVSMMAASAAGAIIPVT
                     LVKLNRDPATASSIILTTVTDIVGFFTFLGLATWFASMLAAS"
     gene            complement(24436..25284)
                     /locus_tag="VFG22_11000"
     CDS             complement(24436..25284)
                     /locus_tag="VFG22_11000"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET6416813.1"
                     /translation="MTLETSLLGEFVSTHPSEAARVLESMPAPDAAEVMQQLPLDLFS
                     ELLRWLSPITAASALESLGPEGAANALSATRSDLAAAVLRTMSAEQQSAIVDRLSPDA
                     RKETGPLLRFADGTAGALMDPSVLTFAESLTAADALERVRHNSEHALYYLYVVDDAQK
                     LVGVANIRELMAARPEQLLGSIAVRTVSSLPARASWESLLAHPGWKRFHALPVVETDG
                     RFLGVVRYESVRKLEDQSAEHQLEDHGARTATALGELYGLGLRGLFDLAASAVLGSGE
                     AEGRPR"
     gene            complement(25268..26113)
                     /locus_tag="VFG22_11005"
     CDS             complement(25268..26113)
                     /locus_tag="VFG22_11005"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416814.1"
                     /translation="MGLDATANQFLELFSSAKQGLVAAFPSIVTAVLVLILGWGLAWV
                     LRKAVRRLFRGVRNQLAPGSARSAWTEAVDDQRTGDVAAGGVYWLVVLTALVIAIDAL
                     GVPVFSRWIGAFAGYLPKVAIAMVVLLGGVIASRLARNAIIKTAIRVPPAQARNLARL
                     GQVSIIVAAALIAAEQLGLDVSLLTAVFLIGLAAALGGAALAFGLGAREVMADILAMH
                     YVQKSYRVGQVVRIGSDQGRIVRTTRTAVVLENIDGELSIPGRHFVDERSVLLTQEED
                     LDSRN"
     gene            complement(26256..26519)
                     /locus_tag="VFG22_11010"
     CDS             complement(26256..26519)
                     /locus_tag="VFG22_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416815.1"
                     /translation="MRTQRTKGGRNRNRGSELDWFAEDPWADPESEAAFEAAELDWSP
                     DSWGSTEEDLDASSLEDDEKYTDDEDADDDDWGPIRPRRNLAK"
     gene            26811..27053
                     /locus_tag="VFG22_11015"
     CDS             26811..27053
                     /locus_tag="VFG22_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416816.1"
                     /translation="MVLDDGELVTPEVLRKIRSENQALLQASQSMQASIETGRFDEAA
                     PIAAALAEVLRKHVLLVDGVLDVLTPDDVVPKRERR"
     gene            complement(27067..28461)
                     /locus_tag="VFG22_11020"
     CDS             complement(27067..28461)
                     /locus_tag="VFG22_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6416817.1"
                     /translation="MGPEHTGQILVVESDGKLQSELEAELTAMGHIVDTAPGGAEALE
                     LVERSMPDVIVVGLELEGMDGFELLRKARALVPSASFIAIGESGSTDTVVKAMRCGAD
                     RYLTRPVRPDALGLVIERSLDKPLQAREAMDAREEAAASLPTTRVDQIVGAHPAIQRL
                     FNRMLQAAKSRSTVLIQGETGTGKELIAKALHEHSQRSDGPLVRLNCAALAESVLESE
                     LFGHEKGAFTGAVTRRKGRFEQADGGTLFLDELSEMPLSVQVKLLRFLQERELERVGG
                     NETVSVNVRVVAATNQDLRALVEDGKFREDLYFRLKVVVLEVPPLRARPSDVPLLADH
                     FLRLYAEENGKEVRGFTPDAREALLTYPWPGNVRELQHAIEQAVVLCQEALIGMEDLP
                     IQPSQSESEPLSLLVPGVTLAEVERYTIMKTLESVGGSPTKAAAILGVSKRTIHYRLQ
                     EWGITKFRSHADDP"
     gene            28564..29424
                     /locus_tag="VFG22_11025"
     CDS             28564..29424
                     /locus_tag="VFG22_11025"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6416818.1"
                     /translation="MSRNVLRSEGSTPPGQTLRARQMLGKYRIQRRIGAGGFATVYAA
                     YDTIEGMPVALKVPHLDRLEKDSLTAIHREVRLTARLEHENILPLRNAMMIDGHLVIA
                     TPLGKHTLTDRLHYRLGPKTALSYAEQLIDALAYAHEMRVIHCDIKPDNVIMFPEGQV
                     RLADFGIAKVALRTRTIMGSGQGTVGYIAPEQAMGKPSFRSDVFSMGLLVYRMFAGEL
                     PAWPFDWPLPGADRLRRTVSRDFIRFLRRSIEVRERKRFRDGVQMQKAFHQVAPKALR
                     DSTRSRRAAP"
     gene            29421..30248
                     /locus_tag="VFG22_11030"
     CDS             29421..30248
                     /locus_tag="VFG22_11030"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HET6416819.1"
                     /translation="MTETRIFTGVDVANGERFDLGSCEAVVLSLMKPGREGPNEDAAA
                     VISLGKDAGVAVVSDGAGGHPGGAVAAAIAVRAVCEAVSRLGDDLEHLRGAIINGFES
                     ADSEISALGTGAAATLVVVEVQGTKMRAYHAGDSMVLVTGQRGRIKWQTIPHSPVGYA
                     VEAGLLDRGEALHHEDLHLVSNLLGTQDMRIELGATLDLAPYDTVVLASDGLSDNLHL
                     DEIVERVRKGPLQRAANELAALGKERMEAPGKGAPSKPDDLTFIAIRRRPVSAKHKS"
     gene            30314..31609
                     /locus_tag="VFG22_11035"
     CDS             30314..31609
                     /locus_tag="VFG22_11035"
                     /inference="COORDINATES: protein motif:HMM:NF014697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HET6416820.1"
                     /translation="MLARLGELFSRWAERWVPDPFVLALVLTAITLLGGFALTGSAAT
                     VFQGWYGGFSSTALLAFALQMCLILVTGQALASSPPVQRLVGALARWPKQTPAAAGLV
                     ALIACLTGLIQWGLGAVVAAFVVREIASSAAEDGRPMDIPVLGAAAYTGLAIWHGGLS
                     GSAPLKAAESAQFTQGMTQAIPVSETLFSSLNFVVSGGLVVLLPLTCMALAPKHALVP
                     EPASRHAVSGSRRKSSLLGVLMGGGAATLVAYAWFRSEVAFDLNSVNLFFLALALAAH
                     GSIRSFLDSVSEAARGAGAIIVQFPLYFGILGVMQASGMIEKLSAAMASVASASTFPL
                     LTFLSAGIVNFFVPSGGGQWALQSDVLLTSAAALDLQSAPTVMAFAYGDEWTNLLQPF
                     WALPLLAITGLKARQIAGYTAVLAIVMGLYVGAVLLLFA"
     gene            complement(31606..32253)
                     /locus_tag="VFG22_11040"
     CDS             complement(31606..32253)
                     /locus_tag="VFG22_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HET6416821.1"
                     /translation="MAWTRDQMAARAAKELRDGFYVNLGIGIPTLVSNHIPEGMSVQL
                     QSENGMLGMGPFPYEGEEDADLINAGKQTVTELPTTSFFSSAESFAMIRGGHIDLSIL
                     GAMQVAENGDLANWMIPGKMVKGMGGAMDLVAGVKRVVVVMEHTAKGKPKLLKECTLP
                     LTGTRVVDLLITDLGVFEIDKAGDGTIVLVELAEGVTLDQIAESTEAAYTVAPGL"
     gene            complement(32264..32968)
                     /locus_tag="VFG22_11045"
     CDS             complement(32264..32968)
                     /locus_tag="VFG22_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HET6416822.1"
                     /translation="MAKKVFPDASAALDGLLSDGMTLMSGGFGLCGIPESLIEAVAKS
                     GVKDLTVISNNAGVDGIGLGVMLETRQIKKMVSSYVGENKLFAKQFLDGDLTVEFNPQ
                     GTLAERIRAGGAGIPAFYTKTGVGTMVAEGKEVREFDGHQYVMERGLFADLAIVHAWK
                     GDTEGNLIYRKTARNFNPMMATAARFTVAEVEHLVEPGELEPDQIMTPGIYVDRIIHF
                     ATPSKHIEQRTTRPRP"
     gene            complement(33013..33870)
                     /locus_tag="VFG22_11050"
     CDS             complement(33013..33870)
                     /locus_tag="VFG22_11050"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HET6416823.1"
                     /translation="MDKAIITCALNGVLTNPKQHPVPVTPKEMAASARDAYNAGASIV
                     HIHFRRQEQGLGHFPSWDPEVAAAASDAIREACPGIIINQTTGVMDDDVSGPIACLHR
                     IKPEIAACNAGSLNYLKLKKDNTWAWPPMVFLNSVEKVQKMLEAMAETHTTAEMECFD
                     VGIVRSVGLYLRAGMAKEMHYNFVMGVASGMPCDPELLELLMKYKSGNAHWQATLIGR
                     EEIWETHQRAADLGGMLRTGVEDTFYLPNGDKTTGNGQLIEALAKCARNAGRDVASPA
                     EARKMLGFN"
     gene            33982..34455
                     /locus_tag="VFG22_11055"
     CDS             33982..34455
                     /locus_tag="VFG22_11055"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET6416824.1"
                     /translation="MVRAVAAPSKTSSGNDRTRAFVEAHGDREAPVFFSIADLADEFG
                     ITHRAIRFYESKGLLAPQRLNGARIYSRRDRARLHIIVRAKSLGYSLEETKDYLDLYG
                     QQGEGRIKQLEISVARSAHMIDELEEKKRQIDEKIEELKLINEVCRKKIAEKKPR"
     gene            complement(34459..36243)
                     /locus_tag="VFG22_11060"
     CDS             complement(34459..36243)
                     /locus_tag="VFG22_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HET6416825.1"
                     /translation="MAIESNPLIDDGLVDFLLYSVLDAESLCKLDAYAQHSKETFDLY
                     IDSIRRFARQDLLPSYKPMDQEPPQFRDGRIHTHPALPKLYEQMVELGTLTATRPESV
                     GGQQMPMLVHTMASAYLMAANLSAYGFIGLTVGAGHLIEAFGSDELKERFMTRLYSGE
                     WTGTMALTEPQAGSSLGDITTSATPTDDGYYLIKGSKIFISGADHGITENIVNLTLAR
                     IDGAPEGTKGISLFAIPKRREENGSLVSNDVTVAGMIHKIGWRGLPSLALEFGDEGDC
                     RGWLVGEPNQGLRFMFQMMNEARIMVGVNGFATASVAYHESRLYALDRKQGRTFGQRG
                     GEPVSIIQHADVRRMLLRQKAFVEGSLALLATVSCYSDVAEHGGSTEERERAQLLLDL
                     LTPVAKTFPAEWGFQANALAIQVLGGYGYTSEYLPESYMRDQKLNTLHEGTNGIQSMD
                     LLGRKVMAKGGAPLRALAEEIGSTIARAREAGCDSDLCSALEEAIAIVGSATMELAQR
                     GMAGDAEGMLGHSWDYLDMFATLVIGWQWLNMAAVSLSAPISEARKSATDAASKYWIR
                     TEVARIPQLAALCRSNEDSYLRAEPGWF"
     gene            36341..37549
                     /locus_tag="VFG22_11065"
     CDS             36341..37549
                     /locus_tag="VFG22_11065"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6416826.1"
                     /translation="MDFEHSERAKNVMAQAERFVRERVVPNEQTYLDQLVGTDDWREW
                     RIPPILEELKAEAKELGLWNLFLPDETYGAGLDNRDYAPVAEITGRSFLAPEVFNCSA
                     PDTGNAEVLVKYGSDEQKKRWLEPLLAGEIRSGFAMTEPAVASSDATNMQATCEVVGD
                     EVVLNGQKWWTSGAGDPRCGFYIFMGVTDPNAGRHARHSMVLVPTDTPGVKTLRMLPV
                     FGHLDEPHGHAEVLFDDVHLPVSSIIAGPGRGFEIAQGRLGPGRIHHCMRAIGAAERA
                     LERLCQRAMLRVAFGKPLANLGGNRDIIANSRMAIDQARLLTMKAAWALDTHGTFGAL
                     VDISAIKVIAPNVLQSVVDAAIQIHGGEGMADPELTRLLAMARALRIADGPDEVHRGM
                     VARLELKKYA"
     gene            complement(37552..38253)
                     /locus_tag="VFG22_11070"
     CDS             complement(37552..38253)
                     /locus_tag="VFG22_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416827.1"
                     /translation="MNKRVCSVAGVLAPLLFISTAAAQGAWSYEPTPTSPYTSTPVPE
                     GPAESTSERSSMDPGLIGSFTLGVPVWLDADRSVVRPGVDLDFFGGVDIGYAVFGLAL
                     GAMWTPINYNDIDGAPPNSGYGRKPMTRLYLAPELRAQIPNKSRLLPYLGVTFDANWW
                     RVRSTDIQCGVYYCRVNAVFLFTPGMTAKIGFAISVSSGALIDIGFKYSLSGPGSFFS
                     GREQWVTPYIGMMFR"
     gene            complement(38250..>38869)
                     /locus_tag="VFG22_11075"
     CDS             complement(38250..>38869)
                     /locus_tag="VFG22_11075"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6416828.1"
                     /translation="AHVLTADLTNPSAPQDIFSALAEKRLQLDYLVNNAGFGANGPFA
                     ELSLQSQMDMLKVNIDAVVALSHLALQGMLARRKGRILNIASTAGFQPGPDMTIYYAT
                     KSFVIMFSEGIAEEVKDAGVTVTAHCPGATATNFAGTAGNDTSILFKLSVSPADKVAR
                     HAYRSMMKGKVVAIEGLLNWLTAFSVRFSPRALVRKLARWMNKTT"
BASE COUNT         7211 a        11950 c        12259 g         7449 t
ORIGIN      
        1 cgacgtccgc gtacatgtcg ggcttgagcg cgaggtccgg gttggcgagg accacgcgca
       61 cgcgagcggt gcgagttgct ccgctcagcg tcgggtagat gtaatccact tgcccctcga
      121 gctcgcgtcc cggcccgtag gggaggtcga ccttcaccgc ttgacctggt tcgagctggg
      181 ccaagtccga ttcgtaaacc tccgcatcga cccaaactct gctcagatcg gcgatccggt
      241 agacacgtgt ccccgcctcg atgcgtccgc cttccacgat gtctttgtcg attacgtatc
      301 cggtcgaggg cgatgctatg ggcacgttct cccaagcgtg cccgcgccgt tcgagctcgc
      361 gaacctgccc ttggctcatg ccgagaagcc gaagccgttg ctcggacgct tggctgagct
      421 ccgaaatcgc gctcgcatcg cctcccggtt tttgcgcgcg ggcggccagg tactcacgtt
      481 gagccgcata gagctcgggg ctgtagagag tgaatagggt ctgaccttct ttgaccttct
      541 ggcctgtctc ggcgacgagt agccgatgga cccagccgct catacgtagg ttcacgtcgg
      601 caagtcgggt ttcgtcgatt gtgattcgac ccacggcgcg aatctggcgg atgatatcgc
      661 ggcgttcgac ttcgctagtt ttgaccccga tctgttgtcg acgatcctca tcgacgatga
      721 tggttccact cccggactca tctttcgcga ccggtgtgag gtccattccg catatcggac
      781 acttgcccgg ctcgtgcttt cgaaccgaag tgtgcatggg gcacgtgtag tactcgatgt
      841 cgccttgctg agcgtgctgt ccgtgtggat cggcgccaga ctcgtgatcg atatttcgct
      901 gtcggacgac ccagaccccg gcggccgcga gtccaagcag acccagaccc atcgcgatcc
      961 aatggcgtcg cctcagagta ccatcaagga gcgtcatgct gtcctccttc agcgccgatg
     1021 cccggcacga ttcctctggc acgatcgagc tccgcggctc tctgcccgag gctcgccaga
     1081 gcctgctcgt attcgatttc gagtgaccga accgaacgct cggcatcgat gagcgcctgg
     1141 aaattgcttc gcccagtttc gtaggcaaac tccgccgcgt cgatctgcgc cttcgctgct
     1201 gggatgagcc gttctcgata gagttgaacc acgtggtgcg cttcgagcac tcgatgtcga
     1261 gcctgatcca cttcgaccct cacttcgtcg tgctgcgaag ccagcgccgc cagtgcctct
     1321 tgagcgcggg cctcggcttg ttccaccgcg cctttccgtg acctgatctg aagcggcagg
     1381 ttgagcgcaa ctccagccat ccagcgatgg tcgttctcgg cccacatgga gttgtactct
     1441 ccgatcacac cgatttcggg ggagtagttg cgtttggcca gcccgaccga tgagtctgcc
     1501 gcatgcagct gtgagcgtgc ggcatgcaac tcgggccgat tgtcgagcgc ctcgtcctgc
     1561 agtgcggatg tgtctgcgat ttcggccgag gcgggagcga tcatcgcggg cggctctgga
     1621 agtggaagct cgggtcgctg gtgcagcagt ccgttgatct gcgcgacgat gacttcacgc
     1681 tctgattcca gcatgacccg ctggtgaatg atgtgggcca actcgacttc ggattgaagt
     1741 acatcctgtt gcgaggcgcg cccagcttcg tattggatca tcgcagtggc tttgatgtcc
     1801 tcgatgagct gacggtgctg atcgttgagc tccagcacac gcgtgactcg gtagtattcg
     1861 tcgaacagag acgacgccat cagggcgaga gcgagacgca tcgcgtcgtg tccgccttgc
     1921 gctgcttcgg cctctgcgag agccacctcg ccggcaaacc ggaacttgcc gggcgctgga
     1981 aaacgctgct cgaggcgaac gacttgcccg aatcgcaccg tgtccgagcg aatgctgagg
     2041 ggcgcaatca catagctcgc catggtgtct tcgagtgagc gcacctgggg gtactccgcg
     2101 agcgcggctc gccacgcctg tcgtgcggcc tcgaggctcg gattgcgtgc gagcaccgca
     2161 cgaacgagcg cctcacgctg aagtggcgct ccttcctcca gaaatgcagg ctcgacggtt
     2221 tgcctctgat tcggcagtcc ctcttgccta gccgactcga gggcgcttcg ctgcgcgtcg
     2281 tagcgcgcgc tctcaccgtg cgcgcagccg ctcattccca aacttcccac cagccacgcg
     2341 acgatggccg tggctatcgg tatacgattc atagctccaa tgctccttct gcggaatccc
     2401 gagggcctgc gcagaacgca ggtccagctc gaccggcgcg gaacgccgat caaccctgac
     2461 gcgcagtgag ctcaagtcag ataggagcag ttccagatga agatcggtgg cccatgggcg
     2521 cccggggagc cgcgcgccag cggaatccac actgtctctt gcgccggctc gacacgcgag
     2581 aaatcagtcg cgagcgctga cagcgagagc atgggcgcat cgacgttgag cctgtcgttc
     2641 gaggaagaga acgggggttg ctcacccttc gaaaacagcg cgccgcaaca atcgtcgacg
     2701 cgcttcagtt gaacgggagg cccgtcgttg gcgtcctggc agcagcaatg cctgagaacg
     2761 agctcttcca ttccacagag gtggagtgcc gctccggcca ccccggcggt ggtcatgaac
     2821 gtcagtgcca atagagctgt ggctgctcgc agcatgcgac tagtcaacag cgtagcacgg
     2881 cccgtttcgt cgcgatagcc gcctgatcag tcgatcccgt gtaggatctg ggcgaaggcc
     2941 acggcctcaa tcgggggtcg gtttccggga ctgattggga gcgtaccagc tggcattgct
     3001 cttgctctag taagagaatc tcgaggcgag atcatgacag accagagcaa cccacatacc
     3061 tgccccatgc atcccgaggt ccggcaagag cgacctggaa gctgcccgaa gtgcggcatg
     3121 gcgctcgaac cagtcggtcc cgccgcgcaa gccacgcata ccgagtggac ctgccccatg
     3181 catcccgaga tcgtgcgagc cgagccgggg agctgtccga tctgcggcat ggcgctcgag
     3241 ccgcgcaacg tcgcctcgga agaagaggag ggcgccaatc cggagctggc cgacatgacc
     3301 cgtaggtttt ggattgccgc ggcgttcacc atccctctat tcctggtcgc aatgggagat
     3361 atgctcccag gagccccggt ttcgcggatc ttgtcgccga gcgcccgcac ctggctcgag
     3421 ctcgggctcg caacgccggt ctgcctctgg gccgcctggc cgttctatgt tcgtttcgcg
     3481 gcgtcgctcg agaacaagag cctcaatatg tttaccttga tcggcctcgg cgtcggtgtg
     3541 gcgtacggat acagcgtcgt cgcggcgctg gctccaggcg tctttcctga agcgtttcgt
     3601 gaaggaggaa cagtggcggt ctatttcgag gctgccgcca tgatcgtcac gttgatactc
     3661 ctcggtcagg ttctcgagtt acgggcgcgg ggccaaactg gcgcggccat caagaagctc
     3721 ctcggcctgg ccgccaagac ggcgaggcgt cttcgcgccg acgggagcga agaggatttg
     3781 ccgttagacg ccgtgcgagt cggtgatcag ctgcgggtgc gtccgggcga gaaggtgccg
     3841 gtcgacgggg tagttcttga agggaagagc gccgtcgacg aatcgatggt cagcggcgag
     3901 ccaattcccg tggaaaaggg tgagggcgat tcggtcatcg gtgccacggt caacggtacc
     3961 ggaatgctcg tcgtgcgcgc ggagaaggtc ggcaacgaga cgcttctatc tcgcatcatt
     4021 gccatggtgg ccgaggcgca gcgaagccgc gcgcccatcc agaagcttgc agatacggtt
     4081 tcgggctact tcgtcccgat cgtgatcggc gtctcagtca cgaccttcgt cgtttggagc
     4141 ctcgtcggcc cagagcctcg catggcgcac gccttgatca acgcggttgc ggttctcatc
     4201 atcgcatgcc cctgtgcgct tggcctcgcc acaccgatgt cgatcatggt cgccatgggg
     4261 aagggcgcga gcaatggagt gctcttcagg gacgccgaag ccattgaagt gatgcgcaag
     4321 gtcgatacgc tggtggtgga caagacggga acgctgaccg agggcaagcc ggtactcgtc
     4381 accgtcgaga ccgttgcggg aatcgacgcg acggagctgc taggtgcggc cgccagtctc
     4441 gagcgcgcga gtgagcaccc tttggcagaa gcgatcgtgg ctggggccgt cgagcggggt
     4501 gcgcatctcg agcgccccga atccttcgat tcgatcaccg ggcagggcgt tcgcggccgg
     4561 atcggtgatc gggacctggc gctcggcaac cggaagctgc tcgaggggat ggacgtggat
     4621 cccggcatcc tcgccgcccg cgcagacacg cttcgcgagg aagggcaaac cgtcatgttc
     4681 gtggtcgtcg atggccgcct gagcggaatc ctcggtgtgg ccgatcccat caaggagagc
     4741 actccggacg cgatcgccga tcttcacaaa gaaggcattc gggtggtgat gctgaccggg
     4801 gacaaccaaa aaactgcgaa tgccgttgct gccaagctgt cgatcgacga ggtcatcgcg
     4861 gaagtccttc ccgatgagaa ggccgaggtc atcaagcgcc ttcagcaggg tggaagcatc
     4921 gtggccatgg cgggcgacgg aatcaatgac gcgccagcgc tggcccaggc ggacgtcggc
     4981 gtggcgatgg gaaccggatc ggacgtggcc atagagagcg ccggcgtcac gctggtcaag
     5041 ggcgacctgc gcggcatcgt tcgcgctcgc aagctcagcc gggcaaccat ggcgaacatc
     5101 aagcagaatc tcttctttgc ttttgtctat aacggtttcg gtgttcccat cgccgctggt
     5161 gtcgcgtacc cgatcttcgg tttgctcttg agccccgtga ttgcggcagc agcgatgagc
     5221 ttcagctccg tgtccgtcat cgcgaactca cttcgcttgc ggcgcctcga gatctgacct
     5281 gcgtcgagct cgatgcgcag ggccctacca gtgaatcccg tgcatgatgt gagcgaatgc
     5341 cacggcctcc ggtccaggcg ctttgtggtc ctcgtccttg ctccctttgc gcggggagtc
     5401 ggggaacagg cggaatccgg tattgaggac ccggtcgatg agcttgggcg cgaccgcgta
     5461 gcttacctct ccgaagatgc cgaggcgggt tgcgacgcgc ttctttcgat tgacgattcc
     5521 atccacgatc atgtcgactg cttcgtcggt ggatcgggtg gggaccgctt cgtagatctt
     5581 cgtcggcgcg atcatgggcg tccgtaccag cggcatgtag accgtcgtga tgcacacgcc
     5641 atcgccgacg acctcactcg cgatcgaacg gctgaaggca tcgagcgcgg ccttgctggc
     5701 gacgtacgcg ctgaacctcg gcgcattggt ttggacgccg atgctggaga tgttcacgat
     5761 ttgtccgtcc ttgcgttcac gcatggtcgg caagaggccg aggatgagct tgagcgagcc
     5821 gaagtagttg agctgcatgg tgcgctcgaa gtcgtggtag cgatcgtagg agtgggctac
     5881 gcctcggcgg atcgagcggc cggcgttgtt gacgagtaag tccacgtgcc cgtacgcgtc
     5941 gagaacctcg cggacgagac gatcgacgtc ttccgagctc gagagatcgc aggtatgaat
     6001 gagcgactcg ccacccctga ggtcgatcgt tcgctggacg gcttcgagct tctccttgga
     6061 acgcgccacc aaaagcactt tggcgccggc ttcggccaag cgaattgcca gggcttcccc
     6121 gatgccgctg gacgcccccg tgaccatcgc gatcttcccg gcgattcgac cgtgcaaggt
     6181 tcggtctagg aagagatccg ggtccatgtg gcgttcccag tagtcccaga tcttccaggc
     6241 gtacgtctcg aaaggagggc aggtgatgcc agtccgggcg agcgcagcct cggtctcacg
     6301 tgtgtcgaat gacgaacgcc agttcacata gggaaggacg gactccggaa tgccaatgtc
     6361 cgcaagaact tcgcgtcgag ccgtcttcac tgcgggcatg gcgccaagca gaccgacgag
     6421 accccggggc acgagtttga ggacgcgggg gtcgatcctc gcgtcgaagc tgggagcgtg
     6481 cgcggcatcg catagcaaat tgagcgcatc gccaaagctc tttggcttcg ggtcggtgag
     6541 atgaaaggta ttgctgctga cttcctcgtt gtgcgcgatg gcgtcgatgg cgcgcgcgac
     6601 atagtccacc ggcacgacgt ggagctccga gccttcgaat ccaatcaacg ggaaccaact
     6661 cggcagggct cgacgcatgc gttggatcag cttgaacgcg tagtagatgc cgtcgatctt
     6721 gtcggcttgc ccatcgatcg atgacccgac gacggcgcct ggtcggtaga ttttgtaacg
     6781 tgtctggcat tcgtcgcgga cgagcttctc ggccaggaac ttggtcttga agtacgggtt
     6841 cttgtgtttc tgcccctcat cgaacatgtc ttcgcggaag aagccgacgt agtcgcctgc
     6901 cactgcggtc gagctcgtgt agtgaagcgt cgcgtcgagt gcttcggcaa gtcgacaggc
     6961 gttccgcgtg cccgtgacgt tggctcgctc gctctcttcc atccccgcgg tcatgtcgta
     7021 gagcgccgcg aggtgaaaca tgtgctgaac cttgtcgcgg agctcctcga tccgttctac
     7081 ggacaagccg caaagcggct cgctgatgtc gcccgcgagc acgacgaact gcgagcgagc
     7141 cgaaggccag cgcttgtcga cgagctccca gaaactcgct tcagaccggg gcaggaccag
     7201 ggcgtagatc gtttggccac ggcgaacgag cgcgtcgagc agatgttttc cgatgaagcc
     7261 gctggctccg gtcacgagat acatgtcacc tcctcaggct gggcccgcga cgatatccgc
     7321 gatgggcccc tcagggcaac acgctcagat cgaacatgtt gatcaggtcc atcgtgagga
     7381 aatcgcgtgc gttttcgccc gccacataga ctccgtcgac cgcatcggtg atcggcgaaa
     7441 gataaaagaa catgccttgc agcccaagcc ggagctccag gttcgagcct tcggcgatcg
     7501 ttcgggggcc ttcggggaac acgacttgtc cgctcatgga gaggctggag gtgactcgaa
     7561 ccaaatcccc agtcatcacc gagctgacct cgacgtccag catttcgccg cggccgcctg
     7621 ccggtgcgag ttggatctga aggccggcgt actcgcccgc ggggacattc gagttgagac
     7681 tcggctcctg ccgagggatc gccagatcga tgatgaccgg tccgagcagc acgacgttcg
     7741 agtctgcgcc gatcagagat accgacagga tggagagggt tcccgcccca agtgtgagct
     7801 ggccgttttc ggaaacgatg ttgtcctggg actcgaccgc taggggaatc tgttccgcat
     7861 cggcggccgg atcgctgcaa gcgcagaacg ctacggcgag gccgaaaagc atgaccttgc
     7921 tgacacaccg agagggcgtt cgcatcatgg gaaactccgg ggtacgaaac ggacagatgc
     7981 accctaggct tcgagagtac atcaaaggcg cgttgctgca taccgacgcc attctcgcga
     8041 ctcgaaaggc cacactcgag gaggtggcgg gtttcgttgc gacgaagcgc tcagaagggg
     8101 ggggcgccga gctcatcttc atttgtacgc acaactcgcg gcgcagtcag atggctgagc
     8161 tctgggctgc ggcggcggct cagcattttg ccatcgatgg cgttcggacg ttctcaggtg
     8221 gcaccgaagc aaccgctttc gatccgcgcg ccgtggcggc cgtcgaacga tcaggcttcc
     8281 tcgtcaaaaa caccggtggc gagaacccgc ggtatcgcat cacgttcgcg gacgacggtc
     8341 tcatactcga gtgtttctcg aagacctacg acgacccttt caatcccgcc cggggctttg
     8401 ccgccatcat gacctgctcc gacgcggacg aagcttgtcc cctcgtcctc ggggccgcgc
     8461 tccgcgctcg tctgccttac gacgacccga aagtcgccga cggcacgccg gacgaaaccc
     8521 tgcaatacga cgcgcgctgc atgcagatcg ctaccgaaat gctctatctg ttctctcggg
     8581 tgcattagcg aacgatcacc gggctcggct ccggaggcgc gcccagagtg cggcggttcg
     8641 acttcggctg ccctccgtcg gcttctcgaa cgccgaggct ctccgcgagc tcagccgcga
     8701 tcaggacgcg gccggaacgc ttcatgcgct gaggatcccg gtgaagcgct tcgatgacgc
     8761 gtccgatgaa ctcggggctc tcagcgtttg cgagaaattt cccgtatggt cctttcatcg
     8821 cgtctggatg ctggaagagc ggctcgttgc gctccgtgcg aacgaaaccg agccataggc
     8881 tcaccgaggc gacgtcgtga tcgcgcaggt cgaccgccat gtccatcgcc atcttgtcga
     8941 tgccagcctt gcctgcaccg tacgcgggtc cgtgcatgta tacgcggccg ccgtagctcg
     9001 aggtaaacac gatcaggccg ccgccggtgt gcaccatcag gggagccgcg aagtaactcg
     9061 cgacgtaatg agagcgcaga ccgacgtcca ggatacgaag gaggtcgagg gacttctccc
     9121 agaacggccc cggcttggcg agctctctcg gaaccgcggt ggcgttgttc accaagatgt
     9181 cgaggcgtcc ttgctcctgc tcgacgcgct ggaacaactc atgcacctgg tcgtcgtcgg
     9241 agtggtcgca ggtgacggcg atgccgtgtc caccggcttc agtgacagcc gccgcagtgc
     9301 tctcgacggt tccgccaagc ggatgttctt ttcccccttg gtgacttcgt cccgtgacgt
     9361 agaccgtggc gccaccagca ccgagggcaa tggcgattcc ttttccgacg ccacggctcg
     9421 accccgtgac gactgcaacc ctttcggagc tcttggtcat cgagctacgc ttctagcacg
     9481 gctgcgggag agaagtaccg cgtccgtatt cgaagggcga cattgacgag cgcgatcatc
     9541 accggaacct ccaccagggg accgatcaca gctgcgaatg cggctccgtg atggatgcca
     9601 aacgtcgcga cggcgacggc aattgcgagc tcgaagttgt tcgacgcggc ggtgaaggag
     9661 aggctcgtcg cgactggata gtccgctccg gccaccttcg agagccaaaa cgagatgaaa
     9721 aacatggcca cgaagtagat gaggagcggt acggcgattc gaaccacgtc gaacggcagc
     9781 gcgacgatcg catcgccctt caaggaaaac atgacgacga tggtgaagag gagggcgacc
     9841 aacgtgattg ggccgatgcg tggcgcgaac gacgcatcgt accaggcgcg cccctttgcc
     9901 cggatgagac cgcggcgtgt gaggaacccg gccgcaaagg ggataccgag gtagatggcg
     9961 acgctcttgg cgatctcgcc gatcgtgatg tccacggcca ttccctcgag accgagcttg
    10021 ccgggcagcc aagtcacgaa gaaccaggcg tacacggaaa agaacagaat ctgaaagatc
    10081 gaattgaagg ccacgagtcc ggcggcaaac tcccggtcgc cgagcgcgag gtcgttccag
    10141 acgatcacca tggcgatgca acgagccagg ccgatgatga tcagaccgac catgtattcg
    10201 ggtttgtccg acaagaacac gaccgccaaa gcgaacatca ggatggggcc gatcagccag
    10261 ttctggacca gggagagcgc gagaactttc ttgttgcgaa agacgcgccc catctcctcg
    10321 tagcggactt tcgcgagcgg cgggtacatc atcaagatca aaccgatcgc gatcgggatc
    10381 gacgtggtgc cgacgctcat cgaatcgagg gcgtcggcga agccaggcac caagtaacca
    10441 agaccgatgc ccagggccat cgcgacgaag atccacagcg tgaggtagcg gtcgagaaag
    10501 gacaggccgc gcttggtcat cgtcatatca gccagctttc catggcggga ggctggagcc
    10561 ttccatatac tctgctccga tgaaagccgc gctcacagag cctcgtccga gcaagtatca
    10621 gattcagggt cgcacggtga ccctgccctg cgtcgttcgg gatgcatgct ccgccacggt
    10681 gacctggctc gtcgactccc gcgccgccca gtccttgttg ccggggcccg atctacagat
    10741 cgccgaagtc gcacctggtc gcggattgct gagcatcgct tgcatcgact acatcgacaa
    10801 cgatctcgga gactacaatg aagtctccat cgcactcttc gtgcggaagc gcggggatcg
    10861 gcgtgggctg ccctatctcg ggtcgatcgt cgacatgatg cgcgggctct tgccgacgca
    10921 catcattcac ttgccggtga atcaatcgtt cacctgcgaa gccgggcgac agatctgggg
    10981 gttccccaag accgtcgacg agatcgactt tgacacgagc ggcgagcgcg ctcgctgcat
    11041 ttgggacagg gacggtcaga acgtcttgaa gttgagtgcg cccatcggag gaaaccgaaa
    11101 atttcccgag caaacactca gcacctacag ctacatcgat ggagctctgc atgagacgcg
    11161 attcgcatcg agcgctgaga gcctcggcat tcgtctggga ggggcggagc tcgagctcgg
    11221 cgcgcatccc gttgctgacg agctgcgtac gctcggtcta cccaagcctg cgctcatgag
    11281 catgtggatg ggaaagatga cagggcgctt cgaggccgcc caacggtgca cttgagccaa
    11341 aggagagaga cgcaatggaa gttcgcgctg cagtcgccca cgaagccgga aaacctctca
    11401 cagtcgagac ggttcaactc gaaggtcccc gggccggtga agtcctaatc gagatcaagg
    11461 ccaccggcgt ctgccacacg gatgcgtaca ctctctctgg cgccgacccg gaaggaatct
    11521 ttccctctgt gctcggtcac gagggtgctg gcgtggtcgt ggaagtcggc gctggcgtgc
    11581 agtcggtgaa accgggcgac cacgtgatcc cattgtacgt tcccgagtgt cggaactgtg
    11641 aatactgcac ctccggcaaa accaatctct gtcaggccgt gcgcgcgact cagggccggg
    11701 ggctgatgcc ggatgcgagc agccgttttt cgatcggaaa cgagacgatc ttccactaca
    11761 tgggcacctc tacgtttgcg cagtacaccg tcgttccgga gatcgcggtc gcaaagatcc
    11821 gcgaggacgc gcctttcgac aaggtttgct acattggctg cggcgtcacg accggcatcg
    11881 gcgcggtcat caacacggcc aaggtcgagc caggcgccaa cgtggtggtc ttcgggctcg
    11941 gcgggatcgg cctcaacgtg gtgcaaggcg cgcgcatggt tggcgcggac aagatcatcg
    12001 gcgtcgacat caaccccgcc cgacgcgagc tcgcagagaa attcgggatg actcacttcg
    12061 tgaacccaac ggaagttgat ggagatctcg tcgcctatct cgtcgacctc accggcgggg
    12121 gcgcggacta tagcttcgag tgcatcggcc gggtcgatgt gatgaggcaa gcactcgagt
    12181 gctgccacaa gggctggggc gagagtgtaa tcgtcggcgt agccggagcc ggtgaggaga
    12241 tttccacccg accgttccag ctcgtaacgg gtcgcgtgtg gaggggaacc gcctttggag
    12301 gagcgcgcgg tcgcacggac gtccccaaga ttgtcgactg gtacatggag ggcaagatca
    12361 acatcgacga cctgatcacg cacaagatgc cgctcgaaga gatcaatacg gccttcgacc
    12421 tgatgcacca gggcaagtcg attcgcagcg tcgtggagct ctgagatggg ctccgagtcc
    12481 gcggttttcg tccaccagtt cgagctgggc ccctgggaca acttcatcta cttcatcggt
    12541 gacaaggcga cgcggaagtg cgcggtcgtc gacccagcct gggactcgga tttcattctc
    12601 gaagaggcgc agcggctgga cgtcgaaatc agcaacatcc tatgcacaca cagccacttc
    12661 gatcacgtga accgagtcga cgccttgctc gagaagcgcg acgtgccggt gcacatgctc
    12721 ggtgaggaga tcgatttttc agggttccac tgcgagaatc tgaagcgcca gagccccgga
    12781 gaccggcttc gaatcgggga gcagctcgag atcagcatgc tgcacactcc cggtcataca
    12841 ccgggttcta ccacttatcg cattcgcgat gcgattgtga ccggcgacac gcttttcatt
    12901 cagggctgtg gccgctgcga cttcgtcggt ggagaccccg agctgatgta ccgaacgctc
    12961 aagactttga ccgaatcatt gccgagagaa acccgcatgt atcccggtca caactacggc
    13021 ccgatgacgg tctccactct cgacgaggag ctcgaggcaa acccttatct tcaacatcag
    13081 accgtccgcg acttcgtgtc tcaccgcatg gaaggcaaga ccccgggcac gcccctccct
    13141 cagccttgac ggcggacaga gtcctccgac ccctaatggg ggtgctttgc acgcgcacgc
    13201 gtcacgcctg tgctcacgta ctgcacggtg ctgatcgcga tcgtgagaag gatcgcggcc
    13261 cctagtacac gcaggacgag catcgggggc tggccgagcc aggcgtgagt gatcgtgagg
    13321 agtacgaaga ggatctgcag caccgtgttg agcttgctgg cgccggtggg ctcgccgtga
    13381 acgggcgcca cgtagacttg atagatgatc gtcccggcga tgatgacgag atcgcgcccg
    13441 atgacgatgg ccgagaacca gaccgggacc cagcctatcc aggccaggct gacgtaggca
    13501 gcaaacatca ggagtttgtc cgccgccgga tcgagcaggc ctccgaggcg cgttcgccaa
    13561 tcgaagcgcc tcgccaagta cccgtccagc gcgtccgaga agccggcgag cagaatgagg
    13621 ccgagggcga gcgcgtagcg accctcgaac aggctccaga cgatcggcgc aacgagaatg
    13681 atgcgtgcga tgcagatcgc gttggggatg tacttgagat tcatgcggat atcgaaccgg
    13741 ccgagccctc cgctcggcgg gccgatggtg gcagacacgg ttcctggagt cgacacctcg
    13801 ttcacgctgg ctgcctcgac gcgagctcat gtgatatggg ctgggcgtgc aaacagaccc
    13861 gacgtctgga gcgagtccgg tgagcgcctt tggggacgac gcgctcggag cgtcagatgc
    13921 tgtagcaatt gcagctcgaa ttcagtccgg agggctccgg gcatgcgatg cggtcgaggc
    13981 cgcgatcact cgagcagagc gcgtgaaccc tgcgctcaac gccatcgtga cgcccctgtt
    14041 cgattccgct cgcgagcagg cgaagagccc gccccccggg accttctcgg gcgttccgac
    14101 gttcatcaaa gacagcgatg ccgtcgcagg gtcccccttc tttttcggct cgcggggtct
    14161 gccccgaaaa ccggcggaga agagctcggc tttcgttcag cagttcttgt cgctgggttt
    14221 gatcaacctg ggaaagtcga gcacgcccga gttcggcctc acgggcacca ccgagccgct
    14281 cgcccatggc ccgacgcaca atccctggaa gttgggattc tctacggggg gatcttctgg
    14341 aggctcggct gcactggtcg cagcgggcgt agtgccgatc gcatatgcaa atgatggggg
    14401 cgggtcgatt cggattcctg cagcgtgctg tgggctcgta gggctcaagc catcgcgcgg
    14461 ccgtctgatc gagatcgaag gctcgaagct ccttccggtg aggctccttc accagggaat
    14521 gctgagccgc acggttcgcg ataccgcagc gttctaccat gcggccgagc gctactatcg
    14581 caatccacgc ttgcccgaga tcggcctggt gacgcggccc ggccggcgct tgcgcatcgg
    14641 tttcttttcg gagttcggag agagtgcgcc tgcacacccg gagtgcgtga ccgcggtaaa
    14701 cgacgtggcg acgctttgcg agggtctcgg ccattctgtg gagcaagtca gagcgccctt
    14761 cgacgaggga tttcacgaag acttcttctt gctctgggca acgatcgcgt tcgtggtcac
    14821 tcgctttggg aaacagctca ttcacccgga cttcgaccgg gcccaggtcg aacccttcac
    14881 tgaaggcttg gcgcagtact ttctcgacaa tcgagtcgat ttcccagcga cggtgtggcg
    14941 attgaggcgt ttcgctcgcc ggtatgcgcg cgggttcgag gactacgacg ttctgatgaa
    15001 cccgaccgtg ggaatgccac cgccggagca cggttacatc ggccccgatg ttcctttcga
    15061 aaccgctctc gaacgcctca agtggttcat tccgttttcg ccggtccaga acgtatcggg
    15121 ggggccagcg atctcgctgc ctctcgctca gagctctcag ggcctgccga tcggtgttca
    15181 gctagcatcg tcgatgggca ccgaacgcac cttgctcgag ctcgcgatcg agctcgaggc
    15241 ggcggctccc tggccgagtc tctagcgtgc gatcagctcg gcgaaaacca acagcgcgac
    15301 actcaatttc cgcatgcact cacctccacc cgagatccgg aggccagact atcctaggaa
    15361 tcggggagcg tctcgaacat ttcgaggtcg gacacatgga cgtaccaggg cgcacgcgag
    15421 ccgaaaaaga cgctcttcgt cggtttgacc cccgggtcgt cgtcgagcgc accagcgccc
    15481 acgaccatcg cctttccgtt tctcgtcagc cagggcacgg ccgagccgca gctcgtgcaa
    15541 aacgcagtgc tccagtattt cgcttcaggg agctcgaaac gcttgacgtt ctcctcgccg
    15601 cgctcccaag cgaactgagt caccgggacg aagatgttgg ccacgtgtga agagccggtc
    15661 ttctttcggc agcggctgca gtggcagtac tgaaacccgc gatagggacc ttcgattcgg
    15721 aacgcgaccg cgccgcagac acaagagccc cgggtcgctc cactcatttc tttgcgtcag
    15781 ccagcgggat gaacttgagc ttctggcctc cgatgctggc gtccgccatc aatccagaag
    15841 ttggaaggat gatgatcgcc acgccgtcgg cgtacttggc cttggccgcg ccaccttgat
    15901 cggcagcgtt tgccgtggcc tgggcgctca gctcgaaacg gtttttcttg aaacgattga
    15961 gagcgctttt ggtcttgaac aagatgatct gtgaaaactt ctggccgccg acctgcgcgc
    16021 cgatcgttcc ttcggagagc tttccgtacc cgatcagctt gccgccctcg tagacttcgc
    16081 cgcgtccgcc ggagccgccc acgatgaatc cgcccttcgc gacctcggga aagatcgcgt
    16141 agcccacgac cgactccgtg gtcttcttga agctgctgtc cttctcctcg aagcgcgtca
    16201 tggcgtcgcg aacatcggaa gcgagcttct cgtcgtcgac ctccttggcc gaggcagggc
    16261 tcgcgctcgc catcatgagg atgcctagga ctaggattgc gcgtgtggtg aaatgcattt
    16321 gcagagtctg gacgcgccgc ctcgaacgtc aaattggggc gccaaccgcc tttttcccga
    16381 gagacgtccc gctggaattc gggtagggtc gagacccttc ctggaggcgg cggtgagcga
    16441 atcgagaacc atgcccggtt acatcgcggc aacggccgcc acgagtcccg accggcacgc
    16501 gctggccgag atgggacccg atgggcgctg ggtcttcacg acctggggcg actactggca
    16561 agccgtgcgg gacttggcaa agggtctgat cgcccttggc ctcgagcccg gggatggcgt
    16621 cgcgctgatc ggcgacaatc gccgcgactg ggtgatctcg cagatgggga tcagcgcggc
    16681 cgcggcggtg cctgcgccga tctacgtgac caataccgtg gagcaggtcg ccttcatcgt
    16741 ccgaaattgc cgtgccaaga tcgcgatctg tgacggtgag gctcagctcc agaagtatct
    16801 cacggcgctc gaccgcggct tgatcgagct cgagcatatc atcgtgatgg acgccatcga
    16861 gagcgagcgc gagatggtgg tctcgctcag ggaggtcatc aagcgtggcc gcggggagcc
    16921 tgacggcgag gtcgagcggc gaatggccga ggccaaaccc gacgatgtcg cgcttctcat
    16981 ctatacctcg ggcacgaccg gagtccccaa gggcgcgatg tgcacgcacc ggggcgtcga
    17041 ggcgatggct gcgtcgttca tggacaagta tccagagctc ttcgaattcg accctcttcg
    17101 ctacgtcagc tacctgccgc tttgccacgt cgccgaacag atcttcacca acttcatcgg
    17161 agtgcgcgtc gcgagcgaaa cgtttttctg cgcggacctc ggcaagatca aggatcactt
    17221 ggtggccgcg cgcccccatt tcttcttcgc cgttccgcgg gtctgggaaa agttcgaagc
    17281 cgcgcttggc gccaatcttc tgtccgcaac gggagcgaaa gcaaagctgg ccgactgggc
    17341 ccggcgggtg gagctgcaaa gcgcgaagcg ctcgatggcg actggaaggc aggtcgacag
    17401 cgtgcgccgc cggctggcca acaagctcgt catttccaaa atcaagagcg ccatcggtct
    17461 cgatgagctc tatgcggcag gctcgggggc cgcgcctgcg tcccgatcga cgctagagtt
    17521 cttcgcgtcg atcggcatca tcattcacga gggtttcgga atgacggaaa cgactggaat
    17581 ggccacggcg cagccgtttg gagcccctca cttcggcagt gtgggccagg cgcttccggg
    17641 cgtcgagatc cggatcgcgg acgacgggga gatcatgctc cgcggaccca acatggtgcc
    17701 cggctacctc tacatgccag aggagacggc atccctgtat gcagaagacg gctggatgca
    17761 cacaggcgac cttggcgtgc tcgacgacga agggtttctc agcatcacgg ggcgcaaaaa
    17821 ggatctgctg attacagcgg gcggcaagaa cgtcgccccc gccgagatgg agaaccacct
    17881 gcaggggatt ccgggggtgg gtcaggccat cgtagtgggc gataggcaac cctatctatg
    17941 cgcgatcctg gcgctcgagg cggaggcggt tcccgagctt tgcagggcgg cgggcgtgcc
    18001 tgaagcccca ttggcggagg tcgcgcgaaa tcagaaggtc cgcgccttcc tcgcggatcg
    18061 agtggaatcc gagtgcaaca gcaaggtcgc gcgctatcaa acgatcaaga aattcgaagt
    18121 gcttccgcac atgttgagcg tcgacggcgg cgagctcacg ccgaccatga agatcaagcg
    18181 caatgtcatc aacgagaagt accgtgccct catcgatggc atgtacgctt cgggcggcgg
    18241 tgactcatcc ccaaaaccgg gctcaacgag ccccgctcgc tgatgatcgg attggctctt
    18301 ccttttcggc gcccaactga gtagaaagtc ccttcatgca gaaattggca ttcgttttcg
    18361 gagccttgct cgctctgtcc ctcgcagtcg tcggacctgc ggtcgcagag gatccggaat
    18421 tcatcatgga ggtcggaacg gttgcgccca aaggaagccc ctgggcggag cagcttcagc
    18481 ggtacaaggc cgaagcgcag gagaaaacgg gaggccggct gaaagtgaag cttcggctcg
    18541 ggcgcggcaa cgagcgctct ttggcccggc gcgttcaaat gggcagcatg caagcgttcg
    18601 ccggctccgt cggcggaatg agctccatcg tacgcgaggt caacgtcatc gaggcgccct
    18661 acgtgttcga gaactttgcg gccgccgaca aggcgctcga cgatccagag gtcaccgcac
    18721 aggtgaagag ggtcctcgag aagcgcggtc tcgtgttcgg gatctgggcg gagaacggct
    18781 accgttccta cttcagcaag atgaagatac gaacgcccga cgacatgaag ggtgttcgct
    18841 ttcgatccca ggagtcgatg gttcacgtcg caacgtacga ggcctttggt gcgacgccgg
    18901 ttccaatcga caatggcaat acgctgatga gtgtgcagac cggtgtggtc gatggcttcg
    18961 acaacacgcc cctcttcgcc atggcgggga gctggtatca aggtctctcc gaaggagaac
    19021 ggaacatctt cctcagccgt cactgctacc agccggcgat cctcgtgtac tcgaagaagt
    19081 ggttcgattc cttgccgaaa gacattcagg aggtcatcac gaccatgccc cacgacctga
    19141 cgatctgggg ccgaaatcaa gtgcgaaaga tcgagcccgt gttgctcaag aacctggagc
    19201 gctacggcta cgacatctac gatccgtcgc cgaaggagct cgaggccttc aaggcaaagc
    19261 agaagaacgt gcccgacaag gtcgccaagg agatgggccc tgaaggggtc gcgctcttga
    19321 aggcgatccg caagactttt tgagccgacc cgagcgtcaa cccgtgacgg tggcaatgtt
    19381 cgcgaccgcg tcgcctcggt ggacgattgg cgaggtaacg tggccgatga ctacaccggc
    19441 acttttcgct ttgacggcca tctgttttct tccgagcgcc ccgaagatga tggcgacggt
    19501 gtcaccttca cgcacttccg ctccgaggga caccatcatt tcgcagaatc cggttcgttg
    19561 cgcgcgaagc caagagcttg aacgtgaaat gcgcggtgcc ggagcagcgg gttcggtaga
    19621 ggaatccaac atgtcgagcg cctgcatcac gcggaggatt ccacgaacgc ccatgtcgat
    19681 cgcgcttgcg ctgaaccggc tcgcctcgcc tgcctcgtag agcagcacgg tctttccaag
    19741 ctcggcagcc gcacctctga gcgagccatc acgcacgcgc gcatgtacga gaatgggcgg
    19801 ggcgaactct cgggcgaggc ggagggtttc ggggtcttcc aggtcgcagc gcagctgtgg
    19861 aaggttcgtt cggccgccgg agccagtatg cagatcgatt cctaccgagg agcgcgcgac
    19921 gatctccgtc atgaacaggt gcgcgagttg ggatgcggag gaaccgcgct tcgagcccgg
    19981 aaagcagcga ttgaggtcgc gccggtcggg caggtagcga gattcctgca cgaggcccaa
    20041 gacgttgacc gccggtacag ccagaaccgt gccggacagc tttgcggggt cgatccgatc
    20101 gagcacgtgc cgaatgatcg gaacgccgtt gagctcatct ccgtgaatcg ccgcgctgat
    20161 ccaaatcacg ggccctgggt tcttcccgtg cacaacgccg acgggcaaag agaaccacat
    20221 gccggtcggg agtcgtgcaa tccgaagctc gacctccgtc agccttcccg ccggagcggt
    20281 gacgccattg acctcgaagg gaggtcttgg cctaaccttg gatccggtct ttggttttcc
    20341 cgcgcttggc attcttttcc aggtattcga tgatgaggcc ggcgacgtcc ttgccgctcg
    20401 acgcctcgat gccctcgagg ccaggtgagg aattcacctc catcacgacg gggccatggt
    20461 tcgatcggag catgtccacg cccgcgaccc gtaggccgag cttcgacgcg gaccgcaccg
    20521 cggtgctccg ctcttccggt gtgagcttca ccgtcttcgc gctcccacct ctgtggagat
    20581 tggatcggaa ctcgccctcg ggtccctggc gcttcatcga tgccacgacc ttgtccccaa
    20641 tgacgaatgc acggatgtct gcaccaccgg cctctttgat gaactcctgt accaggatgt
    20701 tggcgtcgag ttggatgaat gcggacatga ccgactccgc cgctttgtcg gtttctgcaa
    20761 ggaccactcc cattccctga gtgccttcca acagcttgat gatgaccggt gccccgccca
    20821 ccatgtgaat caagccttcg acgtccttcg tcgagcgtgc gaatccggtg acgggcaagc
    20881 cgattccctc gcgggcgagc aattgaaggg cgcgcagctt gtcgcgggac cgggagatcg
    20941 cctgtgagct attgagagag taagtgcgca tcatctcgaa ctgccgcact accgccgttc
    21001 cataaaacgt gttggaggcg ccaatccgcg gaatgatggc gtcgaattcg agtgcgtcgc
    21061 cagacagcaa gacctgagga cgatgcgagg tgatgttcat gtaacatcgg gtgtagtcga
    21121 cgacccttac ttcatggccg cgctcgatcg ccgcctcttt gaggcgcttg gtggaatata
    21181 gcttgccgtt cctcgacaag attccaattc tcatgacggg cctttcttcc ttcgtttctt
    21241 gtgccttttc ggaacgagtt ctcgaaacaa atacgaccga ttgggatcga ccacaaaccg
    21301 ccctcgaatc gcctcgcgac ctagcagcat tcgaaagccc atttcgtcac gcgaggtgag
    21361 ggtcagctcg atgggccagg tctcctcacc caggcgcatc ggagttctga tgatgggtct
    21421 gcgctcgcta tgaccgctac tgctcttcac gttgcggtat tcgtacagac gcaccttgca
    21481 ttgcacgatg gtcaccgagt cgcgctgaat cggatggacc ttgaaatgga cccaggggtg
    21541 cttgccttcg ccggtcacct gaatgtcgaa ggcgtgcagg gacgagctgc gggcgccagt
    21601 gtcgatcttc gccttgatcc gtccgatgcc cagctcagac aaggcaagcc attcgcgcca
    21661 gccaatcgtc gtgctctcat gcggccgaac gcgaattcga ccctcggtgg ccttggtttc
    21721 actcattcga ctcggccctc gagcattgct gcactaagcc gcagattccg ggttctccac
    21781 gcgctcgaag cggagccgaa cgattcggtg ccgaaggact tgcaagacgg tggctttgta
    21841 gtcgccaatc tgcagagtgt cgccgcgctt tggcattcga gccagatagg agatgaagta
    21901 gccggcgatc gtatccgctt cgtcctcgtc gagcgggacc gcgagaaagt cggatgcttc
    21961 cggtagcggg acgttccctg cgagctcgaa gacgcccggg gacacctccg tcatttcgct
    22021 gctctcctcg tcgaattcgt cgtgtatggg gccgacgatt tcctcgagag cgtcctcgag
    22081 gaaggacagg ccgatgatcg ttccatgctc gtcgacgacc gcggcggcgt gggtctgcga
    22141 ccgttgaagc tccacgatga agcgcgacag aggctgcgta tcgggaaccg taggcatccg
    22201 cagcgcttgc gcctcgaggc tcagttcctt ctcctgaagg agagtcgcga ggactgttct
    22261 ggcatgcacg actccccgaa tgttatcgag atcgggatca cccagagggt aacgcgagtg
    22321 acctgtctct cgaaggatac ggagattctc ctccatcgtg ttggaggtgg acaggtacgc
    22381 aacgtcgacc cgtggcaaca tgatgtggcg aacctcgagg cgcatcagcg ccagcacgtt
    22441 gtcgccgatc gatctttgcc gcgcagtgat gtgtccagcg cgcgcagagg acgcgaggat
    22501 ggagcgcagc tcttcgatgc tcgcggacgc atcatgctcg tctgcggtcg acatcccggc
    22561 gaggcggacc aggccattgg aaagcgcgtt gatgaaggag atgagcggtc gaagcgcggt
    22621 tgcaaacgtg tagagggggt atgcaacgaa gagggaaatc gactcggcct tttgaatcgc
    22681 ccagactttc ggagcttgct cgccgacggt catgtgcaga atcgtgatga tggtcagcgc
    22741 aatggcgacc gcaatcagat gtgtggttgg cccgtcatgg atctgccagc ccgcatatgc
    22801 ggctagctcc agcagcaatc gtgcaaccgc cggctccgcc agccagccca gcacgaggct
    22861 ggcgatggtg attccgagct ggcaggcgga aaggtagaga tccaggcgtt gcaacattcg
    22921 ttgaacgatc gccgcgcggc gatcgccgct tcgcacgagc gcgtcgatcc gcattggccg
    22981 gaccttgacg aggccaaact cagccgcaac gaaaaagcca ttcaggacga cgaagatggc
    23041 ggtggccagg agcttccaaa ggacggtagg gtcttgcacg ctcggctagc ctagcaccaa
    23101 ttcgccgcac tcacggccaa gaaaggcaag ccctttagga ggcggcgagc atgctggcaa
    23161 accaagtcgc gagcccgaga aaggtgaaga atccgacgat gtcggtcacg gtggtgagta
    23221 tgatcgagga tgccgtcgct ggatcgcggt tcagcttgac cagcgtgacc ggaatgatgg
    23281 cgcctgcggc ggacgcggcc atcatggata cgatcatcgc cagaaacatc acgagagaaa
    23341 gacccaagtt tcggctccaa gcgtagacgc cgatcgaggt cactgtcgcg atcgcaagcc
    23401 cgttcaccaa tccgatgatg agctccttca cgaggacgcg gaatgcctcg gaaacgcgga
    23461 actcacgcag ggcgagaccc cgcatcgtga cggcaagcgc ctgggcgccg gtgtttcccg
    23521 attggcctgc aacgactgga agcaaaacag ccaccgctgt gaagcgcgcg atcgtatctt
    23581 caaacaggcc gacgaccgat gctgccatga acgcggtgat gaggttgatg ttcaaccacg
    23641 gcaagcgact tcgaacaccg acccaggggc gcgagagcgc gcgctcttcg gtggatccgc
    23701 cgaccatctg ctgcaagccc atgatcgcgt cttcctggac ggcttcgacc agaccagctt
    23761 gccggatgat gccgacgacc ctgttctgga gatctaccac gggcaggctc gaaagctgat
    23821 actcgttcaa gagctccacc acgtcttcgc ggggcgtcat tgggggaacg aatgcaacac
    23881 gcgggttctt gagctcggcg agcggctgtt ccggatcggc ccctacgagg tcttgcaagc
    23941 ggaccacgcc atcgaacttc ccctcgtcgt tcgtcagcac gatgtcggaa atccgtcgtt
    24001 tgcgaagacc ctgaattcgc tcgagcgccg cggaaaccaa agtgtccttg ctgaaaaccg
    24061 ccaccctggc gtccatgaga cgacctgccg tgccggaagg aaacgccaag atcccctcga
    24121 tctctttggc caagctcgcg gaaaccacgc ccagcaaatc agctcgcgtt gcttcgggca
    24181 gacgtgcgag cagattcgct gcgcgggccg ggtcgatcgc tgggatcaag tcacgcgcgc
    24241 ggtcttttcc agctgccgca agaacctctg cagcgtgatc gggttgaacg tagtcgagca
    24301 cggtcgccgc gacctgcggc gattcctcga tcagcacatc cgcaacgtct ttgggagaag
    24361 cgctttcaaa ttttcgagca gcttcggccg gatgcgagcg caggaaatgc ccgactacct
    24421 cggtgaccgc ggagctcatc gcggcctccc ttcggcctct ccagaaccaa gaaccgcaga
    24481 cgccgccaga tcgaagagac ctcgcaggcc gaggccgtag agctcgccaa gcgccgttgc
    24541 cgtcctggcg ccgtgatctt cgagctggtg ttctgccgac tggtcttcga gcttgcgaac
    24601 cgattcgtat cgaacgacgc cgagaaaacg cccgtccgtc tcgacgaccg gcagcgcgtg
    24661 aaagcgcttc cagccggggt gcgcaagaag tgactcccag ctagcgcgcg ccgggagaga
    24721 tgaaacggtg cgaacggcga tcgaccctag aagttgctca ggtcgcgccg ccatcaattc
    24781 acggatgttg gcgacgccca cgagcttctg agcatcgtcg acgacgtata ggtagtagag
    24841 tgcgtgctcc gagttgtggc gaacgcgttc gagcgcatca gcggccgtca ggctttctgc
    24901 aaacgtgagc acggatggat ccatcagggc gcccgcggtc ccgtcggcga atcgaaggag
    24961 cggcccggtc tctttccgtg cgtccgggct caggcggtcg acgatggcgg actgctgctc
    25021 cgcgctcatg gtgcgcagca cggcggctgc cagatcggac cgggtagccg agagcgcatt
    25081 cgcagctcct tccggaccga ggctctcgag tgcgcttgct gcggtgatag gggaaagcca
    25141 tcgaagcagc tccgagaaaa gatcgagcgg cagttgctgc atgacctcgg cggcgtccgg
    25201 agcgggcatc gattcgagca ccctcgctgc ctcgctaggg tgcgtggaga cgaattcgcc
    25261 gagcaagcta gtttcgagag tcaagatctt cctcctgggt caagagaacc gagcgctcgt
    25321 cgacgaagtg ccgccccggg atggagagct cgccatcgat gttctcgagc accacagcgg
    25381 ttcgcgtagt ccgcacgatc cgaccttggt cagatccgat tcgaaccact tggccaaccc
    25441 gataggactt ctgcacgtag tgcatcgcga ggatgtctgc catcacctcc cgcgctccga
    25501 gaccaaaggc cagcgccgcg ccgccaagtg ctgcggccaa gccaattaga aacaccgcgg
    25561 tgagcagtga gacgtcgagc cccagttgct cagcggcgat cagggccgcc gcgacgatga
    25621 tcgacacctg cccgagtcga gccaggtttc gcgcttgggc agggggaact cgaatggcgg
    25681 tcttgatgat cgcgtttcgt gcgagcctgc tcgcgatgac gccgcccagg agaacgacca
    25741 tcgcgatggc gactttcggt aggtagcccg caaatgcgcc gatccatctg ctgaacaccg
    25801 gcacgcccag cgcgtcgatg gcgattacca acgcggtcag gacgacgagc cagtagaccc
    25861 cgcctgctgc cacgtctccg gttcgttgat cgtcgaccgc ctcggtccac gcgctgcgag
    25921 cagaaccggg tgccaactgg ttgcggacgc ctcgaaaaag ccgccgaacg gcttttcgga
    25981 gcacccaggc caagccccag ccaagtatga gaacgagaac ggcggtgacg atgctcggaa
    26041 aagcggcaac gaggccctgc ttcgccgacg aaaatagctc cagaaactga tttgccgtgg
    26101 catcgaggcc catgggactc cccccttgca gggccttacc ttgcacggat ggacctcgat
    26161 tgactacgcg tttttcgtaa ctaggtcgtc caacgccggt ctagtcgcga catgcggcgc
    26221 cttccacatt cggaaaacgc ctttgcctgc aagcgctact ttgccagatt ccgtcgggga
    26281 cggatgggtc cccaatcgtc gtcgtcggcg tcttcgtcgt cggtgtactt ctcgtcgtct
    26341 tccaagctgg acgcgtcgag atcttcctcg gtcgagcccc aggagtccgg gctccagtcg
    26401 agctccgctg cctcaaacgc ggcctcggac tcgggatctg cccacgggtc ttccgcgaac
    26461 caatccaact cgctaccacg gttgcgattc ctgccaccct tcgttcgttg cgttcgcatc
    26521 ggcatgatcg ttctcgcttt tccgggggaa ccatccccta agcgagcgag gagcacgttt
    26581 cgtgccattg gctactccgc tgccaggcgg gcgatcaaac gttgcgcccg agcggcaaaa
    26641 tgcgctcggc gagcccctca gtgcagaagc tgcacacggc atcgcccggg cgaatgcaga
    26701 acttgcactc tgacgcgctg cgcagatcac cccgcgtgct cccgcagccc ttcaatcgct
    26761 agggggattc ggcacgaggt ttgctttggg gggagcccac ggaggattcg atggtcctgg
    26821 atgacggcga attggtcacg cccgaggtgc tgcgaaagat tcgttccgaa aaccaggcgc
    26881 ttctccaggc ttcgcagagc atgcaggcga gcatcgaaac cggacgcttt gacgaggcgg
    26941 ccccgatcgc cgctgcgctt gcggaagtgc tgcgaaaaca cgtgctgctg gtcgacgggg
    27001 tcctcgacgt cctgacgccc gatgacgtcg tgcccaagcg agaacgaaga tgagcccggg
    27061 ttggcttcag ggatcatcgg cgtgcgagcg aaatttggtg attccccact cttgcaaacg
    27121 ataatgaatc gtccgcttgc tcacccctag gatggctgcg gccttcgtcg gtgagccgcc
    27181 gaccgattcg agcgtcttca tgatcgtgta tcgctcgacc tcggcgaggg tgacccctgg
    27241 cacgagcaag ctcaagggct cggactcgct ttgcgacggc tggatgggca ggtcctccat
    27301 tccgatcaat gcttcctggc agagcacgac tgcttgttcg atcgcgtgct ggagctcgcg
    27361 gacgtttccc ggccaggggt aggtgagcag tgcctctctg gcatcgggcg tgaagccacg
    27421 cacttccttc ccattctcct ctgcgtacaa gcggaggaaa tggtcagcca agagcgggac
    27481 gtcgctcggc cgcgcgcgaa ggggagggac ctcgaggacg accaccttga gtctgaaata
    27541 gaggtcctcg cgaaacttcc cgtcttcgac gagtgccctg aggtcttgat tggttgcagc
    27601 gaccacgcgc acgttgacgc tcacggtctc gtttccgcct acccgctcga gctctcgctc
    27661 ctgcagaaac cgaagcagct tcacctgaac cgagagtggc atttcgctca gctcatcgag
    27721 aaagagagtg cctccgtcgg cctgttcgaa gcgccccttt cggcgggtga cggccccggt
    27781 gaacgctcct ttctcgtgtc cgaacagctc cgactcgagc acgctctctg ctagggcagc
    27841 acagttgagg cgaaccagag gcccgtcgct ccgctgcgag tgctcgtgga gagccttggc
    27901 gatcagctcc ttgccggttc cggtttcgcc ctggatgagc acggtggaac ggctcttggc
    27961 ggcctggagc atgcggttga acaagcgctg tatcgcagga tgagcgccga cgatctggtc
    28021 cactcgagtc gtcggaaggc tggcggcggc ctcctcccgg gcatccatgg cctcccgggc
    28081 ctggagcggt ttgtcgagtg agcgctcgat cacgagcccg agggcgtcgg gtcgcaccgg
    28141 ccgggtcaga tatcgatcgg ccccgcagcg catggccttc accaccgtgt cggtggaccc
    28201 ggattcccct atcgcgatga acgaggcaga aggaacgagg gcccgcgctt tccgcaggag
    28261 ctcgaatccg tccatccctt ccaattcgag gccaacgacg atcacgtcgg gcatcgagcg
    28321 ctccaccagc tcgagggcct cggcgccgcc gggtgcggta tccacgatat ggcccatcgc
    28381 cgtgagctcg gcctcaagct ccgactgcag tttgccatcg ctctccacga ccaggatctg
    28441 gccggtgtgc tcggggccca ttcgcgaagg atggcttgac gggagcgcgg cgtccatggt
    28501 ttagcgcagc gggaatacta cgtgcgtcgg caacggggcg atgtcagatt ccgctttggt
    28561 cagatgagca ggaacgtact cagaagcgaa ggaagcactc ccccgggtca aaccctgcgg
    28621 gcccggcaga tgctcggcaa gtaccggatt cagcgccgaa tcggagcggg tggtttcgcc
    28681 actgtctatg ccgcctacga cacgatcgag ggaatgcccg tcgcgctcaa ggttccccac
    28741 ttggataggc tcgagaagga ctcgctcacg gccatccatc gcgaggtgcg actcaccgcg
    28801 cgtctcgaac acgagaacat cctcccgctt cgtaacgcca tgatgatcga cggccacctc
    28861 gtcatcgcaa cgccgctcgg gaaacacacg ctcacggacc gcttgcatta tcgactcggg
    28921 cccaaaaccg cgctgtcata cgccgaacaa ctgatcgacg cgctcgcgta cgcccatgag
    28981 atgcgagtca ttcattgcga catcaagccc gacaacgtga tcatgtttcc cgaggggcag
    29041 gtgcgattgg cagatttcgg aatcgcgaag gtcgcgctga gaacccgtac gatcatgggt
    29101 tcgggccagg gcaccgtggg ctacatcgcg cccgagcagg ccatgggaaa gccgtccttt
    29161 cgctcggacg tgttttccat gggcttgctc gtgtaccgaa tgttcgcggg cgagttgccg
    29221 gcttggccgt ttgattggcc tttgccgggc gcggaccgcc ttcgcagaac ggtcagccgc
    29281 gacttcatca gatttcttcg ccgctcgatc gaagttcggg aacgcaagcg gtttcgcgac
    29341 ggtgttcaga tgcagaaggc tttccaccag gtcgccccga aggcccttcg ggattccacg
    29401 cgcagccgac gggccgcgcc gtgaccgaga cccgcatctt cacgggtgtg gacgtcgcaa
    29461 atggcgagcg attcgacctc gggagctgcg aggctgtggt gctcagcctc atgaaaccgg
    29521 gcagagaagg ccccaacgag gacgctgcag cggtgatttc gctcggcaaa gatgccggcg
    29581 tcgcggtcgt atccgacggc gcgggcggcc atcccggggg cgccgtggcg gccgccatcg
    29641 ccgtgcgtgc agtgtgcgaa gcggtgtcgc ggctcgggga cgacttggag cacctccgtg
    29701 gcgccatcat caacggcttc gaatcagcgg acagcgagat ctccgcgctc gggactgggg
    29761 ctgctgcgac cctggtcgta gtcgaagttc agggcaccaa gatgcgcgcg taccacgccg
    29821 gggattcgat ggtcctagtg accggccagc gtggacgtat caaatggcag accattccgc
    29881 attcgccggt cggctacgcg gtggaggcgg gcttgttgga tcgcggcgag gcgctgcatc
    29941 acgaagacct gcacttggtc tcgaacttgc tgggcactca agacatgcgg atcgagcttg
    30001 gcgcgaccct cgacctggcg ccatacgata cggtggtttt ggccagcgac gggctcagcg
    30061 acaacctcca tctagacgag atcgtcgaga gggttcgcaa ggggcctttg caacgtgctg
    30121 caaacgagtt ggccgctctg ggaaaggagc gcatggaggc gccaggcaag ggggctccct
    30181 ccaagcccga tgacctcacg ttcatcgcca ttagacgccg cccggtctcg gcgaaacaca
    30241 agagctgatc ccgcccccac gtgcgcgtcc ctcgatgcgg tgttgcgcag cagttgatgg
    30301 tatggttgcc gcaatgctcg ctcgacttgg cgaactgttc agccgctggg ctgagcgctg
    30361 ggttcccgac cccttcgtgc tcgcgctcgt attgaccgcg atcactctgc tcggggggtt
    30421 cgcgctgacc ggatccgccg cgacggtctt tcagggctgg tacggcggat tctcgagcac
    30481 ggcgctgctc gcttttgcct tgcagatgtg cctgatcctg gtgaccggtc aggccctggc
    30541 ctcgagcccg ccggtgcagc gtctggtcgg tgcgcttgcg cgctggccga agcaaacgcc
    30601 ggctgcagcg ggcttggtgg cgctcatcgc atgcctcacc ggcctcatcc aatggggtct
    30661 cggcgccgtc gtcgcagcgt tcgtcgtccg cgagatcgcg agtagcgctg cagaagatgg
    30721 gcggccgatg gatatcccgg tgctcggagc ggcggcctat acgggcctcg cgatctggca
    30781 cggaggcctc agtggctcgg ctcccttgaa ggcggcggag tcggcgcagt tcacgcaggg
    30841 catgacgcag gcgatcccgg tgagcgagac actcttctcg tcactgaact tcgtcgtgag
    30901 cggtggactc gtcgtattgc tgcccctcac gtgcatggcc ctggcaccaa agcacgcgct
    30961 cgttcccgag ccggcttccc gtcatgcggt cagcggttca cggcgcaagt cgtcgctcct
    31021 gggcgttctc atgggtggcg gggctgcgac cctcgtcgcg tacgcgtggt ttcgtagcga
    31081 agtcgccttc gacctcaact ccgtcaatct cttcttcctc gccttggcct tggccgcgca
    31141 tggctcgatc cggagctttc tcgattcggt gtctgaagcg gcgcgcgggg ctggagccat
    31201 catcgttcag tttccgctct acttcgggat cctcggcgtg atgcaagcga gcggaatgat
    31261 cgaaaagctc tcggcggcaa tggcctcagt tgcctcggcc tcgacgtttc cgctgctcac
    31321 gttcctgagc gcgggcatcg tgaacttctt cgtcccctcc ggtggcggcc agtgggcgct
    31381 ccagagcgat gttctcttga cgagcgctgc cgccctcgat ttgcagtccg cgcccaccgt
    31441 gatggcgttc gcctacgggg acgagtggac caatctcttg caaccgttct gggcgcttcc
    31501 gctactcgcg atcaccgggc tcaaggcgcg tcagatcgcc ggctacaccg ccgtcctcgc
    31561 gatcgtcatg gggctttacg tcggcgcggt cttgctgctt ttcgcctaga gccccggcgc
    31621 caccgtgtaa gcggcttcgg tgctctccgc gatctggtcg agggtcacgc cctctgcgag
    31681 ctcgacgagc acgattgtgc cgtcgcctgc tttgtcgatc tcgaagactc cgaggtcggt
    31741 gatgagcagg tcgactacac gggtgccggt gagtgggagg gtgcattcct tcagcagctt
    31801 gggctttccc ttcgccgtgt gttccataac gacgaccacg cgcttgactc cggcgaccag
    31861 gtccatggcg ccgcccatgc ccttgaccat cttgcccggg atcatccagt tggcgagatc
    31921 gccgttctcg gccacctgca tcgctcccag gatcgagagg tcgatgtggc cgccgcggat
    31981 catcgcgaag gattcggcgc tcgaaaagaa gctcgtggtc ggaagctcgg tcaccgtctg
    32041 cttgcccgcg ttgatcaggt cggcgtcttc ttcgccctcg taggggaacg ggcccatgcc
    32101 gagcattccg ttttcgcttt ggagttggac gctcatgcct tcgggaatgt ggttcgacac
    32161 gagcgtcggg atcccgatgc cgagattcac gtagaaaccg tctcgaagct cttttgcggc
    32221 gcgagcagcc atttggtcgc gtgtccaggc catcgtcttc tcctcagggg cgtggacgcg
    32281 tcgtccgttg ctcgatgtgc ttcgaggggg ttgcgaagtg aataatccga tcgacgtaga
    32341 tgcccggggt catgatctga tcgggttcga gctcgccggg ctcgacgaga tgctccacct
    32401 cggcaaccgt gaatcgagca gccgtcgcca tcatcggatt gaaattccgg gcggtctttc
    32461 ggtagatgag attgccctct gtatcaccct tccacgcgtg aacgatcgcg aggtcggcaa
    32521 agagcccacg ttccatcacg tactggtggc cgtcgaactc gcggacctct ttgccctcgg
    32581 cgaccatcgt tcccacgcct gtcttcgtgt agaaggccgg tatgccggcg ccccctgccc
    32641 gaatccgttc ggccagggtg ccctgcgggt tgaactccac ggtcagatct ccatcgagaa
    32701 actgctttgc gaagagcttg ttctcgccca cgtacgacga gaccatcttc ttgatttggc
    32761 gtgtctcgag catcacgccg aggccgattc cgtcgacgcc tgcgttgttg gagatgaccg
    32821 tgaggtcctt cacgccagac ttcgcgaccg cttcgatcag tgattcggga attccgcaga
    32881 gcccgaagcc accggacatc agcgtcatgc cgtcgctcag caatccgtcg agggctgcgg
    32941 acgcgtcggg aaataccttc ttggccatgg ctcgtcttta cctccggctt tctcaggtga
    33001 caatgcccgg cttcagttga acccgagcat tttccgggcc tcggcgggcg atgccacatc
    33061 gcgtccggcg ttgcgcgcgc actttgcaag cgcttcgatg agctggccgt ttccggtcgt
    33121 cttgtcaccg ttgggcagat agaacgtgtc ttcgaccccc gtcctcagca tgccgccgag
    33181 atcggccgcg cgctggtggg tttcccagat ctcctcacgt ccgatgagcg tggcttgcca
    33241 gtgcgcattc ccactcttgt acttcatcag aagctccagg agctcgggat cgcagggcat
    33301 gccggaggct acgcccatca cgaagttgta gtgcatctcc ttggccatcc cggccctcaa
    33361 gtagagtccg accgagcgga cgatgcccac gtcgaagcac tccatctcgg cggtggtgtg
    33421 tgtttccgcc atcgcctcga gcatcttctg aaccttctcc acgctgttga gaaacaccat
    33481 ggggggccag gcccaggtgt tgtccttctt caactttagg tagttgaggc ttccagcgtt
    33541 gcacgcggcg atctcgggct tgatgcggtg tagacacgca atcggacccg agacgtcgtc
    33601 gtccatcacg ccagtcgttt gattgatgat gatcccaggg caggcctcgc gaatcgcgtc
    33661 gctggccgcc gcggcgacct cgggatccca ggacgggaaa tgccccaggc cctgttcctg
    33721 acgacggaaa tggatatgca cgatcgaggc gccggcgttg tacgcatcgc gggccgaggc
    33781 tgccatctcc ttgggcgtga ccggcaccgg gtgctgtttc gggttcgtca acacgccgtt
    33841 tagcgcgcag gtgatgatcg ccttgtccat ccgcgtcctc cagattcgag agcctgaggg
    33901 gattctaggc caggttgacg taaacgtcaa ccatagggtt cacaggtaaa tcaactattg
    33961 atgtgacagg cggctagggc tatggtccga gcagtggccg cgccttcgaa aaccagctcc
    34021 gggaacgata gaacgagggc gttcgtcgag gcgcacggcg accgtgaggc tccggttttc
    34081 ttttcgatcg ccgacctggc ggacgagttt ggcatcacgc accgagcgat tcgattctac
    34141 gagtccaagg gcctgctcgc gccgcagcgt ctgaacggtg ctcgaattta ctcgcgccgt
    34201 gaccgcgcac gacttcacat catcgtccgc gccaagtcac tcgggtacag cctcgaagag
    34261 accaaggact acctcgacct ctatgggcag cagggcgaag ggcgcatcaa gcaactcgag
    34321 atatcggttg cgcgcagcgc ccacatgatc gacgagctcg aagaaaagaa acgccagatc
    34381 gacgagaaga tcgaagagct gaagctgatc aacgaagttt gccgcaagaa gatcgccgag
    34441 aagaaacccc gttgagcttc agaaccagcc cggctccgcc ctcaggtacg aatcctcgtt
    34501 gctccggcag agtgcggcca gttgggggat gcgtgcgacc tcggtgcgta tccagtactt
    34561 gctcgccgcg tcggttgcgc tcttgcgtgc ttcggagatc ggagcgctga gcgaaacggc
    34621 tgccatgttg agccactgcc atccgatgac gagggtggcg aacatgtcga gatagtccca
    34681 gctgtgaccg agcatgcctt cggcgtcgcc tgccatgcca cgctgagcga gctccatcgt
    34741 cgcgctgccg acgattgcga tcgcctcctc gagcgctgag cagagatccg aatcgcagcc
    34801 ggcctctcgt gctctcgcga tggtgcttcc gatctcttcc gccagagcgc gcaggggtgc
    34861 gccccctttc gccatcacct tgcgaccgag caggtccatg ctctggattc cattcgttcc
    34921 ctcatgaagc gtgttgagct tctgatcgcg catgtacgat tcgggtaggt actcgctcgt
    34981 gtagccgtag ccaccgagaa cctggatggc cagggcgttg gcttggaagc cccattcggc
    35041 tgggaacgtc ttggccactg gcgtgagcaa atcgaggaga agttgggcgc gctctcgttc
    35101 ctcggtcgac ccgccatgct ccgcgacgtc cgagtagcac gagaccgtcg caagcagtgc
    35161 gaggcttcct tcgacgaacg ctttttgtcg aagcagcatg cgccggacgt cggcgtgttg
    35221 aatgatcgac accggctcac caccacgttg gccgaaggtc cgtccttgct tccgatcgag
    35281 ggcgtagaga cgcgactcgt gataggcgac cgaagccgtg gcgaaaccgt tgacgccgac
    35341 catgatgcgg gcttcgttca tcatttgaaa catgaagcga agtccctggt tgggttcgcc
    35401 gacgagccag ccgcggcaat caccctcgtc accgaactcg agggccaggc ttggcagtcc
    35461 tctccatccg atcttgtgga tcattcccgc cacggtgacg tcgtttgaaa ccaagcttcc
    35521 gttctcttcg cggcgcttcg ggatggcgaa gagcgagatg cccttcgttc cctccggagc
    35581 cccgtcgatc cgggcgagcg tgagattgac gatgttttcc gtgatgccgt ggtccgcgcc
    35641 cgagatgaag atcttgcttc ccttgatcag gtagtacccg tcatcggtcg gcgtcgcgct
    35701 cgtcgtgatg tcgccgaggc tgctgcctgc ctgtggttcg gtgagcgcca tcgttccggt
    35761 ccactcgccg ctgtacagcc gggtcatgaa tcgctccttg agctcgtcac ttccgaacgc
    35821 ttcgatcaag tgcccagcgc ccaccgtgag accgatgaaa ccatacgcgg agagattcgc
    35881 ggccatgaga taggcgctgg ccatcgtgtg aacgagcatg ggcatctgct gcccgcccac
    35941 cgattccggg cgcgttgccg tgagggtgcc cagctctacc atctgctcgt acagtttggg
    36001 cagtgcgggg tgggtgtgaa ttcgcccatc ccgaaattgc ggaggctcct ggtccatcgg
    36061 cttgtagctg ggcagcaggt cttgccgtgc aaaccgccgg atgctgtcga tgtaaaggtc
    36121 gaaggtctct ttcgagtgct gcgcgtaagc gtcgagcttg caaagcgact cggcgtcgag
    36181 aacggagtac agcagaaaat cgacgagacc gtcgtcgatc aaggggttgc tttcaattgc
    36241 catggtttgg acgtgcctcg ctcatcaggt cggaacctag caccggattc ggtgacttcc
    36301 cgcgtgaaat gttcgaaggc atgcggtatc aacgccgtct atggatttcg agcattcaga
    36361 acgggcgaag aacgtgatgg cgcaggcgga gcgattcgtg cgcgagcgcg tcgtgccaaa
    36421 cgagcaaacc tatctcgatc aactcgtcgg cacggacgat tggcgggaat ggaggatccc
    36481 tccgatcctc gaagagctca aagcggaagc caaggagctc gggctctgga atctctttct
    36541 tccggacgag acgtacggcg cgggtttgga caatcgggac tatgcaccgg tcgccgagat
    36601 cacgggccgg agcttcttgg cgcccgaagt gttcaactgc agcgcgcccg acacgggcaa
    36661 cgccgaggtc ctggtgaagt acgggtccga cgagcagaag aagcggtggc tcgagccctt
    36721 gctcgctggg gaaatccgtt cgggatttgc catgaccgag ccggccgttg catcgagcga
    36781 tgcgaccaac atgcaggcga cctgcgaggt ggtgggcgac gaggtggtgc tgaacggcca
    36841 gaagtggtgg accagtggcg caggcgatcc tcgttgcggg ttctacattt tcatgggtgt
    36901 gaccgacccg aacgctggcc gtcacgcgcg ccattcgatg gtgctcgtgc cgaccgacac
    36961 gcccggggtc aagaccttgc gcatgttgcc ggtcttcggt catctcgacg agcctcacgg
    37021 ccatgccgag gtcctcttcg acgacgttca ccttccggtc agcagcatca tcgcggggcc
    37081 gggccgtggc tttgaaatcg cgcagggccg gctagggccg gggcgaatcc atcactgcat
    37141 gcgcgccatc ggagcagcag aacgggcgct cgagcggctc tgccagcggg ccatgctccg
    37201 cgttgcgttt ggcaaaccgc tcgcaaatct cggcggcaat cgggacatca tcgcgaactc
    37261 ccgcatggcg atcgaccagg cgcgccttct caccatgaag gccgcgtggg cgctcgacac
    37321 gcacggcacc tttggtgcgc tggtggacat ctccgcgatc aaggtcatcg ctccgaacgt
    37381 ccttcagtcg gtcgtcgacg ccgcgattca gattcatgga ggcgaaggca tggctgatcc
    37441 ggagctgacc cgcctgctcg ccatggcgcg cgcgcttcga atcgcggatg ggcccgacga
    37501 ggtgcaccgg ggcatggttg cccggctcga gctcaagaaa tacgcatagc gtcagcgaaa
    37561 catcatgccg atgtacggcg tgacccactg ctctcgcccg gagaagaagc tgcccggacc
    37621 cgagagagaa tacttgaacc cgatgtcgat cagcgcgcct gacgacacgc tgatggcgaa
    37681 gcctatcttg gcggtcatgc cgggggtgaa taagaagacg gcattcactc tgcagtagta
    37741 gacgccgcac tggatgtctg tggacctcac gcgccaccag ttcgcgtcga aggtaacccc
    37801 gagatacggc aagagccgcg acttgttcgg gatctgagct cggagctctg gcgccaagta
    37861 gagtcgagtc atcggtttcc ggccgtaccc ggagttcggc ggagcaccgt cgatgtcgtt
    37921 gtagttgatc ggcgtccaca tcgcgccaag cgccaacccg aacaccgcgt agccgatgtc
    37981 gaccccgccg aagaagtcga gatcgacgcc cggccgcacc acgcttcgat cggcgtccaa
    38041 ccaaacggga acgcccaggg tgaacgagcc tatcaggccg gggtccatcg acgacctctc
    38101 gctcgtgctc tcggcggggc cttccgggac cggagtgctc gtatacgggc tggtcggggt
    38161 gggctcgtac gaccaggcgc cttgcgcggc ggcggtcgag atgaagagca ggggcgcgag
    38221 gacccctgcg actgagcaaa ctcgcttgtt catgtcgtct tgttcatcca acgggcgagc
    38281 tttctcacca aggcccgcgg gctgaaccgg acactgaacg ccgtcagcca gttgagcaag
    38341 ccctcgatcg cgacgacctt gcccttcatc atcgagcggt aggcgtgtcg cgcgaccttg
    38401 tctgctggcg agacgctgag cttgaacaag atgctcgtgt cgttccccgc ggtccccgcg
    38461 aagttggttg cggtggctcc ggggcagtgc gccgtgaccg tgacgccggc gtctttgacc
    38521 tcttcggcga tgccctcgga aaacatgatc acgaacgact tggtcgcata gtagatcgtc
    38581 atgtccgggc ccggttggaa ccctgcggtc gacgcgatgt tgagaatccg tcccttccgg
    38641 cgagcgagca ttccctgcag cgccaggtga ctgagcgcaa ccaccgcatc gatgttcacc
    38701 ttcagcatgt ccatttgaga ctgcagcgag agctcggcga atggcccatt ggctccgaac
    38761 ccggcgttgt tgaccaagta gtcgagctgc aagcgttttt ccgcaagcgc cgaaaagatg
    38821 tcctggggcg cgcttggatt cgtcaaatcg gcggtaagca cgtgcgccg
//
LOCUS       DASRZF010000251         3140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_525747, whole genome shotgun sequence.
ACCESSION   DASRZF010000251 DASRZF010000000
VERSION     DASRZF010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525747"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            313..582
                     /locus_tag="VFG22_11080"
     CDS             313..582
                     /locus_tag="VFG22_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416829.1"
                     /translation="MIEPRRHSSINLRRTDESDSLVWEDIRGDVAWQGRWVALDDCAY
                     NDAGETTGGVVVDYDDDLAELCARLSAERRKRCSVVFCRRAESAA"
     gene            complement(600..1103)
                     /locus_tag="VFG22_11085"
     CDS             complement(600..1103)
                     /locus_tag="VFG22_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416830.1"
                     /translation="MKAMQIALALVLGVIIGCGGSHKPKAGPLPEGATFYGVWQSPQY
                     GSMHLCQSGRKVIGDYLKHERAGRIQGDLDGDLLVFQWEDRRELVAGKPQVRRGHGYF
                     RIELGEDGDQYLRGEWGMGDELSGGGPWNAVKLRRGEPDRCTGVDEPVSLEETVHPWD
                     ADDESSD"
     gene            complement(1163..2866)
                     /gene="sppA"
                     /locus_tag="VFG22_11090"
     CDS             complement(1163..2866)
                     /gene="sppA"
                     /locus_tag="VFG22_11090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HET6416831.1"
                     /translation="MLRWLLRTFRNLLCIPVLPFWAISRWLSRPKGRWIALRLKSRLV
                     EEMQPLPWLQRLLDMGSLRRTSSLSAIRKLADYAIRDARLEGVAFVLPPLHAGWSVSA
                     GLREQILRLRDAGKRTTVLLPRGGGHRELYIASAAEQIYLGPQATITLLGLSAQSHYV
                     KPLLDRIGVEVEPFARKEYKTAAERMSRDSMSEPQREQLQALIDGHTEALLGALSQRT
                     GTSPEQARALFEVGVFRGADAIQARLVDGLAYEDELPEKLGLRSEKEKKRGIVEAGKY
                     LAYKESKLFRPARREPYIAVVAVKGAISESGAPSGRRASIVAALREARRDRRALGVLL
                     WVDSPGGSAEASDLIHREVVRLKEKKPVVAYFGEVAASGGYYVAAHANAIVAQPLGLT
                     GSIGVVSARLLASQLLDRVGIRTEVLRTGPHADLFSPHRALTDPERTLMNRELDAFYD
                     SFVALVADGRRRPVEEIEPLARGRVWLAADAHRLGLIDELGGMDVALERLKSAIDAPP
                     RIRERLRPAVLASYRLDPSPPTAQLRLEGSVFEGLRDLAVLLSESPGVLYHVISIPRI
                     R"
BASE COUNT          591 a         1004 c          989 g          556 t
ORIGIN      
        1 tgccgtgacc gagttgcaca cgcggccgga tctggaccgc ttgatcttgg ctctcgccac
       61 ctgattcgat cgcgctccgg ccaacctcga tcccgtgcaa agttccgaca cgcgtgaaca
      121 agatcgctct gtgtcttgct ccaagaagcg cgcaagtgac tgatatcatt gtgtctttct
      181 gattggccct tgaccggaca gaccctcaaa cccaggcttt taattcgctt gcaccgaccc
      241 gagacacggt ggagactcgg cccgctttga gagctttgct gttgatgccg gggaaattcc
      301 aaaggctgca acatgattga accgcgacga cactcatcca tcaaccttcg aagaacggac
      361 gaatcggatt cgctggtctg ggaagacatt cgcggagacg tcgcctggca agggcgttgg
      421 gtcgcactcg acgactgcgc gtacaacgac gccggcgaga ccaccggagg cgtcgtcgtc
      481 gactatgatg acgacctggc cgagctctgt gcgcgcctga gcgcagaacg acgcaagcgt
      541 tgctcggtcg tcttctgtcg ccgcgccgaa tcggccgcgt agccggaatt gtcggtggcc
      601 tagtcgctcg actcgtcgtc ggcgtcccag ggatgaaccg tctcttcgag cgagacaggc
      661 tcgtcaacgc cggtgcagcg gtcgggctcc cctcttcgaa gcttgacggc gttccatgga
      721 ccgccgcccg agagctcgtc gcccatgccc cattcgcccc gcaggtattg gtcgccgtcc
      781 tcgcccagct cgattcggaa gtaaccgtga cctcgacgaa cctgtggctt gccggccacc
      841 agctcacggc ggtcctccca ttggaaaacc agcaagtctc catcgagatc gccctgaatc
      901 cggccggcgc gctcgtgctt caagtagtca ccgatgacct tcctgccgct ctggcagagg
      961 tgcatgctcc cgtactgcgg cgattgccag accccgtaga aggtcgcccc ctcgggcagc
     1021 ggaccggctt tcggcttgtg agagcccccg cagccgatga tgaccccaag cacaagggcc
     1081 agcgctatct gcattgcctt cattgctccc tccccgctcc ccacgataaa cgacccgcgg
     1141 acgaaagcgt aatttcggcg tctcagcgaa tcctggggat cgaaatgacg tgatagagga
     1201 cgcccgggct ctcggagagg agcacggcca ggtcccggag accctcgaat accgatccct
     1261 cgagccggag ctgggccgtg ggcggcgagg ggtctaggcg atagctggcg agaaccgccg
     1321 ggcgaagccg ctcgcggatt cgtggcggag catcgatggc ggacttcagt cgttccaggg
     1381 cgacatccat gccccccagc tcgtcgatca gaccgagacg atgagcgtcc gccgcgagcc
     1441 aaaccctgcc acgcgcgagg ggctcgatct cttcgaccgg ccggcgcctg ccatcggcga
     1501 ccagcgcgac gaacgagtcg tagaaagcat cgagctctcg attcatgaga gtgcgctccg
     1561 gatcggtgag cgcgcggtgt ggcgagaaga ggtctgcatg gggtcccgtg cggagcacct
     1621 cggtccggat gccgacacgg tccagaagct gagaagcgag aagccgagcg gaaacgacgc
     1681 cgatcgagcc agtgagcccc agtggctgag cgacgatcgc atttgcatgc gcggcaacgt
     1741 agtagccgcc gctggccgca acttcgccaa agtaggccac gactggcttc ttttctttga
     1801 gcctcacgac ctcgcgatgg atcaaatcgg aggcctcggc cgatccgccc ggggaatcga
     1861 cccagagcag cacaccaagt gctcggcggt cgcggcgcgc ctcacgaagt gccgcgacga
     1921 tcgaggcccg gcgcccgctc ggcgctcccg actcggagat ggcgcccttc accgcgacca
     1981 cggcgatgta gggttctcgg cgcgcggggc ggaagagctt ggattccttg tacgcgaggt
     2041 attttccggc ctcgacgatg cccctcttct tttccttctc gctcctgagg cccagcttct
     2101 cggggagctc gtcttcgtac gcgagaccgt ccacgagacg cgcttgaatc gcgtctgcac
     2161 cgcggaacac gccgacctcg aacagcgccc gcgcttgctc cgggctggtc ccggtccgtt
     2221 gggagagcgc gccgagcagt gcctcggtat gcccgtcgat caacgcttgc agctgctcgc
     2281 gctggggctc gctcatcgag tcgcggctca tgcgttccgc cgcagtcttg tactccttgc
     2341 gcgcgaacgg ctcgacctcg acaccaattc gatcgagaag cggtttgacg tagtgtgact
     2401 gcgccgaaag accgaggagc gtgatcgtcg cttgggggcc gagatagatc tgctccgcgg
     2461 ctgacgcgat atagagctcg cgatgcccgc cgccccgcgg aaggaggacc gtggtgcgct
     2521 tgccagcgtc cctcaagcga aggatctgct cgcgcagacc tgccgagacc gaccagcccg
     2581 cgtgaagcgg aggcagcacg aacgcgacac cctccagtct ggcatccctg atcgcataat
     2641 cagcaagctt gcggatcgca gagagcgagg aggtgcgcct cagcgagccc atgtcgagca
     2701 gcctctggag ccacgggagc ggctgcatct cctccacgag gcgcgatttg agccttaatg
     2761 cgatccagcg tcccttgggc cgggagagcc agcgggagat cgcccagaat ggaaggacgg
     2821 gaatgcagag cagatttcgg aacgttcgaa ggagccaacg aagcatcgtt tttgtcttta
     2881 gtgacggctt gttatgctgg aagtggtggg gccggaaatc tttaccggcg actagcgctc
     2941 tgctaggaac tcatagcact gaagaaggcg aaggagcacg gctcaaaaca tggcaagccc
     3001 ccagatggtg atgtacgagg aggagtttgc ccaggtccag gccgtgaccg acaggctggt
     3061 tcaggatgca aacgcgcgcg tcgtgttcgt cgtcgacaag aacggccaac tcatcagcgc
     3121 ttccggtgac atcggggata
//
LOCUS       DASRZF010000252        13480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1561508, whole genome shotgun sequence.
ACCESSION   DASRZF010000252 DASRZF010000000
VERSION     DASRZF010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 13480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13480
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1561508"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VFG22_11095"
     CDS             <1..352
                     /locus_tag="VFG22_11095"
                     /inference="COORDINATES: protein motif:HMM:NF019453.4"
                     /note="catalyzes the formation of pyruvate and
                     acetaldehyde from 4-hydroxy-2-ketovaleric acid; involved
                     in the degradation of phenylpropionate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HET6416832.1"
                     /translation="AGMGAGAGNTPLEVFVAVLHRMGVTTGVDMWKLMDVAEDIVYPM
                     MTHKVRADRDSLTLGYAGVYSSFLLHAKRAAEKYGLKSTDILVELGRMKTVGGQEDMI
                     EDVALDMKKAGAAE"
     gene            357..1562
                     /locus_tag="VFG22_11100"
     CDS             357..1562
                     /locus_tag="VFG22_11100"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6416833.1"
                     /translation="MSELDDFRASARDWVRANAPKSLWGTSGSGPFEGYWGGSKCEET
                     DADILRWRDLCIEKAWTAPSWPREYGGGGLSHEQENIIHAEMKAIGVPRPIAGQGLAM
                     FGPVLLEYGNDAQRAQHLPGIAHGEVRWCQGYSEPNAGSDLANLQLKAEHDGDELVLN
                     GQKIWTSHANLSDWIYCLARTDPHAPKKQMGISVILASLETPGITVRRIDLISGYSPF
                     CETFFDDVRVPLGNLVGIENEGWAIAKAILGHERSSIGRSIARQPGSAQREVLARARK
                     HLGVEDGPIPDPLLRDELASLGMLEEAFHLTLARIQQSAESGAPGPEGSITKIVGTEL
                     KQLRFELGMKVAGTQGLGWEGPGFDEEDLQYTRDWLRSRASTIEGGSSEIQKNIVAKR
                     VLALPEGKK"
     gene            1559..2716
                     /locus_tag="VFG22_11105"
     CDS             1559..2716
                     /locus_tag="VFG22_11105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6416834.1"
                     /translation="MSDLRAPSLLLNEEQKMLSRTAHEFIRDRAPAARLRAFRDSKDE
                     LGFSRELWSEMAKLGWLGLQIPEEYEGLGLGFFDLCVVLEQAGRELMPEPFVSTLLLG
                     TQALLLAGSPAQKKAFLPGIAAGEKIVAFGHEERCFGRHPERLESLAQPVGERFELNG
                     EKVQVLDAHVADAIIVSAATGMSDESTRATLFLVDPGQEGVSITRQHRIDGQNAAIVR
                     LEGVLVDRGAVVGEVDAGESLIESILDRASVGLAAQMLGGSEQAFADTLEYLKEREQF
                     GVPIGSFQALQHRAVSVYTEIALTRSVVLAAARAIDESPEQVPKLASLAKANASETFI
                     HAAKEAIQMHGGIGVTDEHDIGFYMKRAQATYMTFGRPSQHRRRWAELHGY"
     gene            2745..3497
                     /locus_tag="VFG22_11110"
     CDS             2745..3497
                     /locus_tag="VFG22_11110"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6416835.1"
                     /translation="MKRFEGKNVVITGAAAGIGQATAQRIAEEGGNVALIDINAEGLA
                     ATKRIAEGLGVKAWVYECNVADGARVKEVIDQAAEDMGGFDTLCHCAGILRTYHTHLM
                     TFEQWHQIIDINLNGTFYVNQAALPHLLKNRFSALVNCSSTSALGSHPWMSAYAASKG
                     GILSMTRALYQEYVKQGLRANCVIPGGIATSLHGDFKAPEGADMELLKGAMPFVGFAK
                     PKHAASVIAFLASDDARYINGTEIRADGGALS"
     gene            complement(3522..4154)
                     /locus_tag="VFG22_11115"
     CDS             complement(3522..4154)
                     /locus_tag="VFG22_11115"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HET6416836.1"
                     /translation="MQIAVALFEGVTALDAVGPYEVLQRLPSSAVHFVGHRRGEVRTD
                     NGFLGLTVDKLFDEVRSPDVLLVPGGIGTRALVHDDEILNWIRGAHETSRFTTSVCTG
                     SLLLAAAGLLNGLRATTHFSARALLAKYGATPSEDRVVQEGKIITAAGVSSGIDMALR
                     LAAQLADETTAKALQLMIEYDPQPPYDAGALHKVDAAVLARVQALSKDRS"
     gene            complement(4161..5894)
                     /locus_tag="VFG22_11120"
     CDS             complement(4161..5894)
                     /locus_tag="VFG22_11120"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HET6416837.1"
                     /translation="MPRKRVSRLFDLKEDEHGRKYMEVYLDGIALLRLVLTNKGTAFS
                     HEERLALRLGGLLPPQYNTLEQQIDRVYQGFVQAPTDIDKYQYLRAVQERQEILFYAL
                     LEKHLDEMLPIVYTPTVGAAVQQFSALYQNPRGLSYSPLNIDRAAQTLECYPMEDVRM
                     IVATDSSAILGIGDQGYGGLAIPIGKMALYTVGGGVSPFHTMPVALDVGTDRMDLIND
                     PNYLGVRQKRLRGDDYYKFLDQFVDAVWSRWPDAVIQWEDLSKTAAFTVLERYRDRGP
                     SFNDDIQGTGAVALAGILAACHLRNESLKDEVFVIHGAGAGGVGVAWAVREGLVRAGL
                     SRAEAKLRVFVLDSQGLLLSDREMEQYKQDFAQERAAIRGWEFEGDYPDLVETIRNAK
                     ATVLLGLSGRPGAFDEPVVRAMADNHKWPIIFPLSNPTSSAEAKPIDIFRWTDGQAIV
                     ATGSPFDPVMMGGVTHPIGQGNNAFIFPGLGFGAILSDAKSITDGMVMAASQALAEYT
                     IEKYVEGGLIYPPVNDLQETSVRVTTAVLKQAMADGVARRENIPEDLEAFVRGRFWHP
                     TYLPFVRGRLD"
     gene            complement(5884..6984)
                     /gene="leuB"
                     /locus_tag="VFG22_11125"
     CDS             complement(5884..6984)
                     /gene="leuB"
                     /locus_tag="VFG22_11125"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HET6416838.1"
                     /translation="MKAKITLLPGDGIGPEIVDATRQVLEAVASKSGHAFTYSTHLIG
                     GIAIDETGVPLPDDTIVACRSSDAVLLGAVGGPKWDDPSAKVRPEQGLLGMRKALKLF
                     ANVRPVKLFKGLEAASPLKAERLRGVDLVFIRELTGGIYFGQPKGKEVRNGLRTGFDT
                     MVYDEAEIRRIVELAFEVARKRGGHVTSIDKANVLATMQVWREVATEVGKENPDIKLD
                     HQLVDSAAMRLVTDPASFDVIVSGNMFGDILSDEGAVLTGSLGLLPSASLGEGTLGVY
                     EPIHGSAPDIAGRGIANPLGTILSAAMLLRHSLNLDEDAKRIEDAVQKVLDSGLRTAD
                     LSGGRRSLGTSEMADAVVAAIDGAPAKEHHAP"
     gene            complement(7040..8659)
                     /gene="cimA"
                     /locus_tag="VFG22_11130"
     CDS             complement(7040..8659)
                     /gene="cimA"
                     /locus_tag="VFG22_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HET6416839.1"
                     /translation="MSNDGHNGAGPVVEVYDTTLRDGTQMEGMSLSCGDKLRIAEHLD
                     SLGVAFIEGGWPGSNPKDVEFFERAKDIPWKNVAIAAFGSTCRAQLRAEDDPQLKTLL
                     AAETPVCTIFGKTWNMHVTEVLRTTLEENLRMVEESVAFLSRNDRRVIYDAEHFFDGY
                     KADSGYALETLRAAERGGAETLALCDTNGGSMPWEVEKIVKEVREALPETRIGIHAHN
                     DGDCGVANSVTAIRAGARHVQGTINGYGERCGNANLCAIIPDLELKLGLGCVPEGGLA
                     KLYDLSHFVAEIANLSPNEHAAYVGRSAFAHKGGVHVSAIRRHPESYEHVNPTLVGNE
                     SRVVVSELSGRGNVLSKAEELGLEVSSAHGGRVLEEIKNLEAKGFAYEAAEASVALLM
                     ARHQEDYEAPFEVLDFQAGVGRRRGGGMFAEATVKVRIGDEVVHTAGEGNGPVSALDS
                     ALRKALRPFFPEVAEMHLSDYKVRILDGRSGTGATTRVLIDSQSSRDSWSTVGASSNI
                     IEASLQALIDSIEYGLMLERARLAEPTAART"
     gene            complement(8640..9227)
                     /gene="leuD"
                     /locus_tag="VFG22_11135"
     CDS             complement(8640..9227)
                     /gene="leuD"
                     /locus_tag="VFG22_11135"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HET6416840.1"
                     /translation="MAKFTTLTSHAVPLPAENVDTDQIIPARFLKVTDKSGLGDSLFC
                     DWRYLKDGSDNPDFVLNEAASQGAQILVAGNNFGSGSSREHAPWALVGWGFRAIISTG
                     FADIFKSNALKNGLLPVEVSPEVYKSILKAVQLDRATKITIDLPTQTVTGPGGLEASF
                     PIDAFSKHCLLQGIDQLGYLLSFGEEISAYEQRRT"
     gene            complement(9239..10648)
                     /gene="leuC"
                     /locus_tag="VFG22_11140"
     CDS             complement(9239..10648)
                     /gene="leuC"
                     /locus_tag="VFG22_11140"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HET6416841.1"
                     /translation="MEPRTLFQKVWDEHVVVQEPDRPAVLYIDLQLIHEVTSPQAFTG
                     LRERGIRVRRPEKTVATMDHSTPTLPKSLWVIDDQAKAQLGQLTSNCEEFGIELFPLG
                     DSRNGIVHVIGPERGFTQPGKTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQCLL
                     QKKPKTLEVRVDGQLMPGVTAKDVILALCAEIGTGGATGYVIEYTGSAIRGLDMEGRM
                     TVCNMSIEAGARAGMIAPDQTTFNYLKGRERVPQGPDWDAAVERWSQLRTDDGAAFDK
                     TVVIDANKLEPMITYGTNPGMGMQLSAQMPTTDAFDDPSQRAAFEKAMRYMGLEPGQP
                     LLGKKIDVVFIGSCTNSRIGDLRMAAGVFSGRKVADGVRTLVVPGSHDIKKQAEAEGL
                     DRIFKEAGAEWREPGCSMCIAMNGDNLEPGQYAVSTSNRNFEGRQGKGGRTFLASPLT
                     AAAAAVTGQVTDPRSLLLS"
     gene            complement(10657..12294)
                     /locus_tag="VFG22_11145"
     CDS             complement(10657..12294)
                     /locus_tag="VFG22_11145"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HET6416842.1"
                     /translation="MQPNHVRIFDTTLRDGEQSPGATMTSSEKLEVAKTLSRLGVDII
                     EAGFAAASLDDLRAVKTIAEAVGVTGVEGRPASEPPTICSLARAHKPDIDKAAEAVAP
                     AKHGRIHTFLATSPIHREHKLRMSRDEVLAKISEMVAYARSFCEDVEFSPEDAGRTEM
                     DFLYEALQAAIEAGASTLNIPDTVGYTIPNEFGERIAMIRENVPGIEKAIISVHCHND
                     LGLAVANSLSAVQNGARQVEVAINGIGERAGNASLEEVVMALRVRHDLYGIETNVDAT
                     QLTRASRLVSKVTGMVVQPNKAIVGANAFAHEAGIHQDGMLKHEQTYEIMRPETVGAG
                     KTQLVLGKHSGRHAFANRLTELGYPVAGDGLDKAFARFKKLADKKKHITDADLVALVS
                     DELYQPKEFWRLEKIQVSCGTGMPTATVRLTGPDGRTQTVAEVGTGPVDAVYKAIQVL
                     VDVPCTLLEYTVQSVTEGIDALGEVSVRVSPHDGDAPPDRINPQYEQTRARVFHGHGA
                     HTDVIVASAKAYLAAINRILAIREAKQNESNAAGSAA"
     gene            complement(12333..13397)
                     /gene="ilvC"
                     /locus_tag="VFG22_11150"
     CDS             complement(12333..13397)
                     /gene="ilvC"
                     /locus_tag="VFG22_11150"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF004017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HET6416843.1"
                     /translation="MPPATRPHNPFEEVERHMTQIRYEKDADLSLIRGKKVAIIGYGS
                     QGHAHALNLKESGVDVRIGLQDGSKSKAKAEAAGLAVRSVSDAAKEADVIMILAPDTS
                     QPAIYEEHIAPNLTAGKMLMFAHGFNIRYGTIEPPKNVDVAMVAPKSPGHRVRETFQE
                     GGGVPALLAVHQDATGHADALAMSYAAGIGCARAGVLTTTFTEETETDLFGEQAVLCG
                     GASELVKAGFETLVEAGYQPELAYFECLHELKLIVDLMYRGGLNYMRYSVSTTAEWGD
                     YVSGPRVIGAESKKAMKQILAEIQDGTFAKRWIEENRKGCPEFNKIRHAEQEQRIEQV
                     GAKLRSMMPFVDPVEIKQGE"
BASE COUNT         2452 a         4102 c         4126 g         2800 t
ORIGIN      
        1 cgcgggtatg ggagcagggg cgggcaacac gccgctcgaa gtgttcgtag cggtgctcca
       61 tcggatgggt gtcacaaccg gagtcgatat gtggaagttg atggatgtgg ccgaagacat
      121 cgtctaccca atgatgacgc acaaggtccg cgcggaccgt gactcgttga cgctcggcta
      181 cgcgggtgtc tactcgtcgt ttttgctaca cgcgaagcgt gccgccgaaa agtacggcct
      241 caaatcgacc gacattctcg tcgagctcgg acggatgaaa accgtaggcg gccaggagga
      301 catgatcgag gacgtcgcgc tcgacatgaa aaaggccggt gcggcggagt gatcgcgtga
      361 gtgagctgga tgatttccgc gcatcagcgc gggactgggt tcgcgcgaac gcgcccaaat
      421 cgctttgggg caccagcggc agtggtccat tcgaaggcta ttggggcggt tcgaagtgcg
      481 aggagaccga tgccgacatc ttgcgttggc gcgacctctg catcgagaag gcctggaccg
      541 ctccctcttg gccgagggag tatggcggag ggggcctcag ccacgagcag gagaacatca
      601 ttcacgcgga gatgaaagcg atcggtgttc cacgtcccat cgccggtcag ggcctcgcca
      661 tgttcggtcc ggttctgctg gagtacggca acgacgctca gagagcgcag cacctacccg
      721 gaattgccca cggcgaagtc cgctggtgtc aggggtactc cgaacccaat gcaggttccg
      781 atttggcaaa ccttcagctg aaggccgaac acgacggcga cgagctggtc ttgaacgggc
      841 agaagatttg gacttcgcat gcgaacctga gtgactggat ctattgcctt gcccgcaccg
      901 acccacacgc tcccaagaag cagatgggta tctcggtgat cctcgccagt ctcgagacgc
      961 ctggaatcac ggtgcgccga atcgatctga taagcgggta ttcgccgttc tgcgaaactt
     1021 tcttcgatga tgtccgcgtg ccactcggaa atctcgtggg catcgagaac gagggctggg
     1081 ccatcgccaa ggcgattctc ggacacgagc gctcttcgat cggccggtcg atcgctcgac
     1141 agccggggtc agcacagcgc gaggttctcg cgagggcccg aaagcacctc ggcgtggagg
     1201 acggacccat cccagacccc ttgctgcgcg acgagcttgc gtcgctcgga atgctggaag
     1261 aggcctttca cctcacgctt gcccgtattc aacaaagcgc ggaaagcgga gctcctggac
     1321 ccgaggggtc gatcaccaag atcgtgggga ccgagctgaa acagctgcga ttcgagctcg
     1381 gcatgaaagt ggccggaacc cagggcctcg gctgggaagg tccgggtttc gacgaggagg
     1441 atcttcagta cacccgtgac tggctgcgtt cgcgcgcgag caccatcgaa ggaggttcct
     1501 cggaaattca aaagaacatc gtcgccaagc gagtgcttgc gctcccggag ggaaagaagt
     1561 gagcgatttg agggcgccct cccttcttct caacgaggag caaaagatgc tctcacgaac
     1621 tgcgcacgag ttcattcgcg accgcgcacc ggccgcgcgg ctccgagcgt ttcgcgactc
     1681 gaaagacgag ctcggattct cgcgcgagct atggtccgag atggcgaagt tgggctggct
     1741 tgggttgcag ataccggagg aatacgaagg tcttgggctc ggatttttcg atctctgcgt
     1801 cgtgctcgaa caggccgggc gtgagctcat gcccgagccc ttcgtatcca cgttgttgct
     1861 tgggacgcag gcgctcttgc tcgcgggtag tcctgcccag aagaaggcgt ttctgcctgg
     1921 gattgctgcg ggtgaaaaga tcgttgcgtt cgggcacgaa gaacgatgct tcggcagaca
     1981 tcctgagagg ctcgagtccc tcgcccaacc ggttggagag cgcttcgagt tgaacggaga
     2041 aaaggttcag gttctcgacg cccacgttgc ggacgcgatc atcgtatcgg ccgcaactgg
     2101 tatgtcagac gagtccactc gagcgacgct cttcttagtg gaccccggtc aggagggtgt
     2161 gagcatcacc cgtcagcacc gaatcgatgg gcagaatgca gcgatcgtgc ggctcgaagg
     2221 cgtcttggtc gacaggggcg cagtcgtggg tgaagtcgat gcgggggagt cgttgatcga
     2281 gagcattctc gatcgtgcaa gcgttggcct cgctgcgcag atgttgggag gttccgagca
     2341 agcctttgca gataccctcg agtacctcaa ggagcgggag cagtttggcg ttccgatcgg
     2401 atcgttccag gcacttcagc accgcgcagt gtcggtctac accgagatcg cgctgacgcg
     2461 ctcggtggtt ctggcagctg cgcgtgccat cgacgaatct cctgagcagg ttcccaagct
     2521 tgcctccctc gcgaaggcca atgcatcgga aaccttcatc catgcggcca aggaagcgat
     2581 tcaaatgcat ggtgggattg gcgtaaccga cgagcacgac atcggctttt acatgaagcg
     2641 cgcgcaggcg acttacatga ctttcgggag gccctctcag caccggcggc gctgggcgga
     2701 gcttcacggt tactagtgag catccaggaa gagcggggaa tacgatgaaa aggtttgaag
     2761 gaaagaacgt ggtgatcacg ggcgctgccg caggtatcgg ccaggccacc gcgcagcgca
     2821 tcgccgaaga gggcggcaac gtcgctctca tcgacatcaa cgcagaggga ctcgctgcga
     2881 caaagcggat cgccgagggc ctcggggtca aagcctgggt ctacgagtgc aacgtcgccg
     2941 acggtgctcg cgtcaaggag gtgatcgacc aggccgcaga ggacatggga ggtttcgaca
     3001 cactctgtca ctgcgccgga atcctccgaa cgtatcacac gcacctgatg acttttgagc
     3061 agtggcatca gatcatcgac atcaatctca atggcacgtt ctacgtgaac caagcagcgt
     3121 tgccgcatct tctgaagaat cgattcagcg cgctggtcaa ttgctcgtcg acctcagccc
     3181 tcggaagcca cccttggatg agcgcctatg cggcatcgaa aggcggtatc ctctcgatga
     3241 cccgcgcgct ctaccaagag tacgtgaagc agggtcttcg agcgaactgc gtcatccccg
     3301 gcggcatcgc gacctcgctc catggcgact tcaaggcgcc cgaaggcgcg gacatggagc
     3361 tgctcaaggg ggccatgccg ttcgtaggct tcgcgaagcc caaacacgcg gcgtcggtca
     3421 tcgcattcct ggcttcggac gacgcccggt acatcaacgg caccgaaata cgtgccgacg
     3481 gtggcgcact ctcctagggg atccgctgcg tcgacgcgtg gtcaggacct gtccttcgac
     3541 aaggcttgca cgcgcgcgag cacagcggcg tcgactttgt gcagggcgcc agcgtcatac
     3601 ggtggctgcg ggtcgtactc gatcatgagt tgaagagctt tcgcggtcgt ttcatccgcg
     3661 agttgcgcgg cgagacgcaa tgccatgtcg attccactcg agaccccggc cgcagtgatg
     3721 atctttcctt cttgaacgac gcgatcttcc gatggcgtcg cgccgtactt cgcgagcaag
     3781 gcccgggcgg agaagtgggt cgtggcgcgc agcccgttca agagacctgc ggcggcgaga
     3841 agtagcgagc ccgtgcagac cgaggtcgtg aaccgactcg tctcgtgtgc cccgcgaatc
     3901 caattgagaa tctcatcgtc gtggacgagc gcccgcgtcc cgataccgcc gggcaccaga
     3961 aggacgtcag ggctccggac ttcgtcaaac agcttgtcga ccgtgagccc caagaacccg
     4021 ttgtccgttc gcacctcacc ccttcggtgg ccgacgaaat ggaccgccga gcttggtagc
     4081 cgctgcagaa cttcgtatgg ccccacagcg tccagcgctg ttaccccttc gaaaagcgct
     4141 actgcgatct gcacgagtcc ctagtccagg cggccgcgga cgaaggggag gtaggtaggg
     4201 tgccagaagc gccctcggac gaaagcctcc aggtcttccg gaatgttttc acggcgggca
     4261 acgccatcgg ccatcgcttg tttgagcacg gcggtcgtga ctcggaccga ggtctcttgt
     4321 aggtcgttga cgggtgggta aatgagcccg ccttcgacat acttctcgat ggtgtattcg
     4381 gccaaggcct gcgaggctgc catcaccatc ccatcagtga tggatttcgc atcgctcaga
     4441 atcgcgccga agcccagacc tgggaaaatg aaagcgttgt tcccttggcc gattggatgc
     4501 gtcacgccgc ccatcatgac cggatcgaac gggctaccgg tcgctacaat cgcctgccca
     4561 tcggtccagc ggaagatgtc gatcggtttg gcctctgcag agctcgtggg attcgacagt
     4621 gggaaaatga tcggccactt gtggttgtct gccatcgcgc gtacgaccgg ttcatcgaaa
     4681 gcaccaggcc ggcccgaaag accgagtaag accgtcgcct tcgcgttccg aatggtctcg
     4741 accaagtcgg ggtagtctcc ttcgaactcc caaccccgga tggcggcgcg ctcctgagca
     4801 aagtcttgct tgtactgctc catctcgcgg tcgctgagca gcaggccttg cgagtcgagc
     4861 acgaagacgc gcaacttggc ttccgcgcga ctcaggccgg cccgaacgag tccttctcgc
     4921 actgcccagg cgaccccgac accgccggcg cccgctccgt ggatcacgaa gacctcgtct
     4981 ttcaggctct cgttcctcaa gtgacaggca gccaaaatgc ccgccaaagc gaccgcgccc
     5041 gtgccctgga tgtcgtcatt gaacgacgga ccgcgatcgc ggtagcgctc gaggaccgta
     5101 aaggccgccg tcttagagag gtcttcccac tgaatcactg cgtcgggcca tcgtgaccag
     5161 actgcatcga cgaactggtc gaggaacttg tagtagtcgt ctcctcgaag tcgcttctga
     5221 cgaacaccta ggtagttcgg gtcgttgatg agatccatcc ggtcggtgcc gacgtcgagt
     5281 gctacgggca tggtgtggaa cgggctcacg ccgccgccga ccgtgtagag ggccatcttg
     5341 ccgatgggga ttgcgagacc gccatagcct tgatcgccga ttccgaggat ggcggaggag
     5401 tcggtcgcaa cgatcatgcg aacgtcttcc atcgggtagc attcgagggt ttgcgccgcg
     5461 cgatcgatgt tgaggggcga gtacgaaagc ccgcgcgggt tctgatagag cgcactgaat
     5521 tgctgcacgg ccgcaccgac ggtcggcgtg tagacgatgg gcaacatctc atcgaggtgc
     5581 ttctcgagta gcgcgtagaa gagtatctct tggcgttcct ggaccgctcg caggtactga
     5641 tacttgtcga tgtcggtcgg ggcctgcacg aaaccttggt acactcggtc gatctgttgt
     5701 tcgagtgtat tgtactgagg agggaggagg cctccgagtc ggagagcgag ccgctcctcg
     5761 tggctgaacg ccgtgccttt gttggtcagg acgaggcgca gaagagcgat cccatcgaga
     5821 tagacctcca tgtacttgcg tccatgttcg tcttccttga ggtcaaacag gcggctaacg
     5881 cgtttacggg gcatggtgtt cctttgctgg tgcgccgtcg atcgcagcta cgaccgcatc
     5941 ggccatttcg ctcgtgccga gcgagcgtcg tccgccgctc agatccgctg ttctcagccc
     6001 gctatctagc accttctgta cggcatcttc gattctcttg gcatcctcat cgaggttgag
     6061 actgtgccgg agtagcatcg cggcgctcaa gatcgtaccg agtgggtttg cgattcctct
     6121 tcctgcgatg tcgggcgccg agccgtggat cggttcgtac acgccgagtg tcccttcgcc
     6181 cagcgacgcg ctcgggagaa gcccgagcga gcccgtcagg accgcaccct cgtccgaaag
     6241 tatgtcgccg aacatgttgc cgctcacgat gacatcgaac gaggccgggt cggttacgag
     6301 tcgcatggcc gccgaatcga cgagctgatg gtccagcttg atgtcggggt tttccttccc
     6361 aacctcggtg gctacttcgc gccatacctg catcgttgcg aggacgttcg ctttgtcgat
     6421 cgaagtcaca tggccgcctc gctttctcgc aacttcgaag gcgagctcga cgatgcgacg
     6481 aatctcagcc tcgtcataga ccatggtgtc gaagcccgtg cgcagcccgt ttcgaacctc
     6541 tttgcccttc ggctgtccga aatagatccc gccggtcaac tcacgaatga ataccaagtc
     6601 gacgccacgc aatcgctctg ccttgagcgg ggaggctgcc tccagacctt tgaaaagctt
     6661 cacgggtcgc acgtttgcga acagcttgag cgccttgcgc atcccgagga gtccttgctc
     6721 tggacgaact tttgccgacg ggtcgtccca ctttggaccg ccgacggcgc cgagcaagac
     6781 cgcatccgag cttcggcaag caacgatcgt gtcgtctggg agcggaacac ccgtttcatc
     6841 tatcgcgatg cctccgatca ggtgtgtgga gtacgtgaag gcgtggccag acttgcttgc
     6901 gaccgcctcg agcacttggc gtgttgcgtc gacgatttca gggccgattc cgtcgcccgg
     6961 gagaagtgtg atcttggctt tcatctactg ttacctatcg gccggccatc atgcacggca
     7021 ggtgtcattg gatcaatggc taagtccggg ccgccgtcgg ttccgcgagt ctcgcgcgtt
     7081 cgagcatcaa cccgtattcg atactgtcga tgagcgcctg tagcgacgct tcgatgatgt
     7141 tgctcgatgc tccgaccgtg ctccacgaat cgcgagacga ctggctgtcg atgaggacac
     7201 gcgtcgtcgc gccggtgcca cttcgcccgt ctagaatgcg tactttgtaa tcgctgaggt
     7261 gcatctccgc gacctctggg aaaaacggcc tgagcgcctt tcgtagcgcc gagtcgagcg
     7321 cactcactgg accgtttcct tcgcccgccg tatgcaccac ttcgtcgccg atccgcacct
     7381 tcacggtagc ttccgcaaac atgcctccgc ctctccgtcg tccgacgccc gcctggaagt
     7441 cgagcacctc gaacggcgct tcgtagtctt cctgatggcg ggccatcaac agggcgaccg
     7501 acgcttccgc ggcttcgtat gcgaagccct tcgcctcgag gttcttgatt tcctccagca
     7561 cacgtccacc gtgggcggag ctcacttcga gaccgagctc ttcggccttg ctcagcacgt
     7621 taccacggcc cgaaagctcg ctgaccacaa cccgcgactc gttgccgacc agcgtcggat
     7681 tgacgtgttc gtacgactcc gggtgacggc gaatcgcgga cacgtgcacc ccgcctttgt
     7741 gggcaaatgc gctacgtcca acataagcgg catgttcgtt cggactcagg ttggcaatct
     7801 ctgcgacgaa gtgcgagagg tcgtaaagct ttgcgaggcc gccttccgga acgcaaccca
     7861 agcctagttt gagctcgagg tccgggatga tggcgcagag gtttgcgttt ccacatcgct
     7921 cgccatagcc gttgatcgta ccttggacgt ggcgcgcgcc agcacggatt gcggtgacag
     7981 agttggccac accacagtcg ccgtcgttgt gcgcatggat gccgattcgt gtttccggaa
     8041 gggcctcacg aacttctttg acgatctttt cgacctccca cggcatcgaa ccgccgttcg
     8101 tgtcgcagag cgccaaggtc tcggctccgc cgcgctcggc cgcgcgcaaa gtctcgaggg
     8161 cgtaccccga atccgccttg tatccgtcga agaaatgctc ggcgtcatag atcactcgac
     8221 ggtcgttccg actcaggaat gcgacactct cctcgaccat ccggaggttc tcttcgagag
     8281 tcgtccggag cacttccgtg acgtgcatgt tccaggtctt gccgaagatc gtacaaaccg
     8341 gcgtctctgc cgcaagtaag gtcttcagtt gtgggtcatc ctctgcacgc agttgcgccc
     8401 ggcacgtcga gccgaatgca gcgatggcga cgttcttcca agggatgtct ttggcacgct
     8461 cgaagaactc gacgtccttc gggttcgaac cgggccatcc tccttcgatg aaggcaaccc
     8521 ccagtgaatc caggtgttcg gcgattcgca gtttgtcgcc gcaagacagg ctcatgccct
     8581 ccatctgcgt tccatcgcgc agagtggtgt cgtagacctc cacgacgggg ccggcgccgt
     8641 tatgtccgtc gttgctcata cgcgctgatc tcctcgccga aggagaggag gtacccaagt
     8701 tggtcaatcc cctgaaggag gcaatgcttg gagaaggcat cgatgggaaa gctcgcttcg
     8761 agccctccgg ggccggtgac cgtctgggtg ggtaggtcga ttgtgatctt ggtcgcgcgg
     8821 tcgagctgga ccgccttcag gatcgacttg tagacctcgg ggctcacctc tacgggcagc
     8881 aatccgtttt tcaaggcgtt gctcttgaag atgtcggcaa atccagtcga gatgatcgcg
     8941 cgaaagcccc agccgacgag tgcccaagga gcgtgctcac gagaggagcc gctgccgaag
     9001 ttgttaccgg cgacgaggat ctgggcgccc tgcgaagccg cttcgttgag aacgaagtcc
     9061 ggattgtccg agccatcttt caagtagcgc cagtcgcaaa aaagactgtc accgagaccg
     9121 cttttgtccg tcaccttgag aaagcgagca ggaatgatct ggtcggtgtc gacgttctct
     9181 gccggaagtg ggaccgcatg cgaggttagc gtagtgaatt tcgccatgac tcagcctttc
     9241 aggaaaggag caagctccga ggatcggtga cctggcccgt gaccgctgcc gcggccgcgg
     9301 tgagtgggga tgcgagaaac gttcggccgc ctttcccttg tcttccctcg aagtttcggt
     9361 tcgaagtgct gaccgcatac tgcccaggtt cgaggttgtc gccgttcatc gcgatgcaca
     9421 tggagcaacc aggctcgcgc cattcggcgc cggcttcttt gaagattcga tccagccctt
     9481 cggcctccgc ctgcttcttg atgtcgtgag atcccggaac gacgagggtg cgcacgccat
     9541 ccgcaacctt ccgtccactg aagacgccgg ctgccattct caggtcgccg attcgcgagt
     9601 tcgtgcacga cccgatgaac actacgtcga tcttcttgcc gaggagcggc tgaccaggct
     9661 ccagtcccat gtagcgcatc gctttctcga aggcggcgcg ttgcgaaggg tcgtcgaagg
     9721 cgtcggtggt cggcatctgt gcgctcagct gcatccccat gccaggattc gtcccatagg
     9781 tgatcatcgg ttccagcttg tttgcgtcga ttacgacggt cttgtcgaag gccgcgccat
     9841 cgtcggtgcg cagttgactc cagcgttcga ccgcagcgtc ccaatcgggg ccctgcggaa
     9901 cgcgctcgcg tcccttgagg tagttgaagg tcgtttggtc tggagcgatc atgcctgcgc
     9961 gcgcgccggc ctcgatgctc atgttgcaaa ccgtcatccg gccttccatg tccaggccgc
    10021 ggatcgcgga cccggtgtac tcgatgacgt agccggtcgc tccacccgta ccgatttcgg
    10081 cacagagcgc caaaatgacg tccttcgcgg tgacacccgg catcaattgg ccgtcgactc
    10141 gcacttctag ggtcttcggc ttcttctgca ggaggcactg agtcgccaag acgtgctcga
    10201 cctcgctggt gccaatgccg aacgccaatg cgccaaatgc gccgtgcgta gacgtgtgac
    10261 tgtcgccaca gacgatggtc ttccccggtt gcgtgaagcc acgctccggc ccgatcacgt
    10321 gcacgatccc gtttcggcta tcacccagcg gaaaaagctc gatgccgaac tcctcgcagt
    10381 tgctcgtgag ttggcctagt tgcgccttcg cctggtcatc gatgacccag agcgacttgg
    10441 gaagcgtcgg ggtcgaatga tccatggttg cgacggtctt ctcgggccgc cgaacccgga
    10501 tcccacgctc tcgaagaccc gtgaacgctt gcggggacgt cacctcgtga atgagctgca
    10561 gatcgatgta aaggaccgct ggcctgtccg gctcctgaac gacgacgtgt tcgtcccaaa
    10621 ccttctgaaa tagtgttctt ggttccatga tatccgtcat gcagcgctac cggccgcgtt
    10681 gctctcgttc tgcttggctt cccgaatcgc gaggattcgg ttgatcgctg cgaggtaagc
    10741 cttcgcgctc gcgacgatga cgtcggtatg tgcgccatgg ccgtggaaca cccgcgctcg
    10801 cgtttgctca tactgggggt tgattctgtc tgggggcgca tcaccgtcgt gtggcgacac
    10861 gcgcacgctc acttcgccga gggcgtcgat accctcggtc acggactgaa cggtatattc
    10921 aagaagtgtg cacgggacat ccaccaagac ctgaatggcc ttgtacaccg cgtcgaccgg
    10981 accagttccg acttcagcga ccgtctgggt tcgcccgtcg ggaccggtga ggcgaacggt
    11041 cgcggtgggc attcccgttc cgcaactcac ctgaatcttt tcgagccgcc agaactcctt
    11101 gggctggtag agctcgtcgc tcaccagagc caccaagtcg gcgtcggtga tgtgcttctt
    11161 cttgtccgcc agcttcttga atcgcgcgaa cgctttgtcg agcccgtcac ccgcgacggg
    11221 gtatccaagc tcggtcaaac ggttggcgaa ggcatgccgg cccgagtgct tgccgagaac
    11281 cagttgtgtc tttccggcgc ccaccgtttc gggccgcatg atttcgtagg tttgctcgtg
    11341 cttgagcatt ccgtcctggt gaatgcccgc ttcatgagca aatgcgttgg ccccgacgat
    11401 tgctttgttt ggctgcacga ccattccggt gaccttgctc accaggcgac tcgcccgcgt
    11461 gagttgggtg gcgtccacat tggtctcgat accatagagg tcgtgccgca cccgaagcgc
    11521 catcacgact tcttcgagcg aagcattgcc ggcccgttca ccgattccgt tgatcgcaac
    11581 ttcgacctgt cgtgcgccgt tttgcaccgc ggacaggcta ttggccactg ccagtccgag
    11641 gtcattgtgg cagtggacgc tgatgatcgc cttctcgatg cccgggacgt tttcgcgaat
    11701 catcgcgatc cgctctccga actcgttggg aatcgtgtag ccgaccgtgt cggggatgtt
    11761 caaggtgctc gcaccggcct cgattgccgc ttgaagcgcc tcgtagagga aatccatctc
    11821 tgttcgaccg gcgtcttctg ggctgaactc gacgtcctcg cagaagctgc gcgcataggc
    11881 aaccatctcg cttatcttcg cgagcacttc gtcccggctc atgcggagct tgtgctcgcg
    11941 atggatgggc gaggtggcca gaaacgtgtg gatccggccg tgcttggccg gcgccaccgc
    12001 ttcagctgct ttgtcgatgt cgggcttgtg agctcgcgca aggctgcaga tggttggcgg
    12061 ctcggaagcc ggacgaccct cgaccccggt cactcccact gcctcggcga tcgtcttcac
    12121 cgcgcgaagg tcgtcgagag aagcggccgc aaaacccgct tcgatgatat ccacacccag
    12181 tcgcgatagg gtcttcgcca cctctagctt ttccgacgag gtcatcgtcg cgccaggtga
    12241 ttgctctccg tcgcggagcg ttgtgtcgaa gatccgtacg tgattgggtt gcatcgttca
    12301 ctctcccgac gccgcttcac gcggcgccca gctcattctc cctgtttgat ttccaccggg
    12361 tcgacgaagg gcatcatcga ccgaagcttt gcgccgacct gctcgatgcg ctgttcctgc
    12421 tcggcgtggc ggatcttgtt gaattccgga cagcctttcc tgttctcttc gatccagcgc
    12481 ttggcaaagg tgccatcctg aatctctgcg agtatctgtt tcatcgcctt cttgctttcc
    12541 gcgccgatca cgcgcgggcc gctgacgtag tcaccccatt ccgccgtggt ggatacggag
    12601 tagcgcatgt agttgaggcc gcctcgatac atgagatcga cgatcagctt cagctcgtgg
    12661 agacactcga agtatgcgag ctcgggctgg tagccggctt cgacgagcgt ctcgaatcca
    12721 gctttcacga gctccgatgc gccgccgcag aggacggctt gttcgccgaa gaggtcggtt
    12781 tcggtttctt ccgtgaaggt cgtcgtcaag acaccggcgc gcgcacatcc gattccagcc
    12841 gcgtaggaca tcgccagggc atcggcatgg ccggtcgcgt cctgatggac ggcaagcagc
    12901 gctggaacgc cgccaccctc ctggaaagtt tctcgaacac ggtggcccgg gctcttcggc
    12961 gcgaccatcg ctacgtccac attcttcggc ggctcgatgg tgccgtaccg gatgttgaag
    13021 ccgtgcgcga acatcagcat cttgcccgcc gtcaggttgg gcgcgatgtg ctcctcatag
    13081 atcgccggct gcgaggtgtc tggcgccaag atcatgatta cgtcggcttc cttggccgcg
    13141 tcgctcaccg aacgcaccgc aagaccagcc gcctcggctt tcgccttgct cttgctgcca
    13201 tcctgcaagc cgatgcgtac gtcgacgccg ctttccttca agttgagcgc atgcgcgtgg
    13261 ccctgagagc cgtagccgat gatcgccacc ttcttgcctc gaatcaacga gaggtcggcg
    13321 tctttttcat agcgaatctg ggtcatgtgt ctctctactt cttcaaaggg gttatgcggc
    13381 cgagttgcgg gaggcaggca ccgtggggtg ggctagaccg tgcttttcga cgccgcgagt
    13441 catcgcgacg gtgccggttt ggaccaactc caggatgcca
//
LOCUS       DASRZF010000253        14039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2940055, whole genome shotgun sequence.
ACCESSION   DASRZF010000253 DASRZF010000000
VERSION     DASRZF010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 14039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14039
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2940055"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VFG22_11155"
     CDS             <1..288
                     /locus_tag="VFG22_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HET6416844.1"
                     /translation="DAGVGGAVAEVIGRKIHEVAQHSQVLCITHLPQISAYADAHYRV
                     HKEVVGKRTKSDIRLLTKNERLEEIARMLGGVTVTDQARAAAEALIQSAAT"
     gene            complement(732..1244)
                     /locus_tag="VFG22_11160"
     CDS             complement(732..1244)
                     /locus_tag="VFG22_11160"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HET6416845.1"
                     /translation="MGVTTFLRAEGLTTESKTNFVREVWNRLERVPGGKLLYSKLVGR
                     LAPYTGSIGAMVQELGDGYSRVTLADRRSVRNHLSCVHAVALANLVELTGNLALGYSL
                     PDDARFIVAGMSLDYIKKARGTITGECRMSPVETSAKREYEVKVTLRDEGGDIVVEGT
                     LRTLVGPKQL"
     gene            complement(1401..2516)
                     /locus_tag="VFG22_11165"
     CDS             complement(1401..2516)
                     /locus_tag="VFG22_11165"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6416846.1"
                     /translation="MDFSFSEDQNSIRELVKQVLSDIVTHDSLKALAKESIWFHERAW
                     KQLAESEMLGLAIPEEYGGAGMGLTELCILLQQVGRTVAPIPAVETLASSALPIAKFG
                     TDEQKRRFLPDIAEGETILTAALVDSGSRNPLEPSALARTEREGWKLTGRFSNVAYAD
                     LAARILVPARTEAGEIGVFLVDPKAKGVRLGAQRGTNGQPLWFLELSDAAVAASDRLG
                     SANEGSNVLRFLVDRTNLAICAVEHGIAEQALFMTAKYSGERKQFRVPIATFQGVTQR
                     VGDAYIDVQAMRSSMWQACWRLDQGLNADKELAVAKYFASEAGARVVAAAQHVHGGMG
                     FDRDYPLYRYFMTSKHLELLLGGAHESLTRLGDLLSK"
     gene            complement(2528..3706)
                     /locus_tag="VFG22_11170"
     CDS             complement(2528..3706)
                     /locus_tag="VFG22_11170"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6416847.1"
                     /translation="MYFDYTPEQKALRDELRAYFSTLITEELLAELDEAEGGGPLYHE
                     AMQKLGADGWLGIGWPKEHGGQGRGPIEQFIFFDEVQRAGFPVPILTLNTVGPTLLKF
                     GTEEQKAEYPPKILEGRCHFSIGYTEPGSGTDLASLQTSAVRDGNQYVINGQKIWTSL
                     ADHADYMWLACRTDPDAPPHKGISIIIVPADAEGVQITPMHALGENNVHAVYYDNVRV
                     PIGNIVGGENNGWHLITTQLNHERVALCSVGPLEKICTETIEWAKKTPNGEGGMVIDL
                     PWVKTNLARVYAKLEVLKLLNWQQAWGIAKDALSPADASAIKVFGSEFYVEGSRLLME
                     VHGAAGALHHSSEGAIVRGRLEKYYRMSLVLTFGGGTNEIQRDIICQAGLRMPRLGRR
                     "
     gene            complement(3853..4209)
                     /locus_tag="VFG22_11175"
     CDS             complement(3853..4209)
                     /locus_tag="VFG22_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416848.1"
                     /translation="MTKHIFRLGLVLAICGAMIGCKKAEDAAGEAAEAADHAAHQAEE
                     AAEGAAEAAEVAVEAADEAMEAAGEAAEEAQEAVEGAAEEAQEAAEGAADDVKAAADE
                     AEAKVKAGADAAKKKY"
     gene            complement(4499..6523)
                     /locus_tag="VFG22_11180"
     CDS             complement(4499..6523)
                     /locus_tag="VFG22_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416849.1"
                     /translation="MPEARAVPEVGVEGQPSRPQRDRALWWAIGGAVVVAALAFEWSR
                     RYKYNVCDDSLISLQYARNVAQGLGFVFNSGERVEGFTNFLWVSLLALAHPLSRGSSA
                     AFVHLATALSVLLAALDIVLLYRLGRLVWPRRLVPIVLMLGLCVFDNGYTVWAMQALE
                     SHLLIFAMLAACLFLWGTPSHWSAIGAALSLAAAMMTRPDAALFVAVLGVSELVFAFQ
                     SERRRPALLRAIAIFGSTAVVFGVYFGWRYQYFGYLLPNTFYVKVSGLRGEAIERGLR
                     YLDDFLRDRAYVPLLSLGAVLGIRDRVIGPLLAWALLYSAYVVYVGGDFYPGHRFLVV
                     LIPVTALLSAYTLERAASFVRARASSAPRLSWAVYALGAALLAMVAVRGYRVGPVQTE
                     ITRWGHEVARVRGLMEWLGAQAPPGASIVTGDIGSSGFYANLYVYDFFGVIDPITAHQ
                     DQTRFGRGKAGHEKRADPDYLLSKHPTYIKRGYIRRDLYRYGYYLDANIPAELDEPGI
                     WTRDELNEDNGWTEVAGISFEAVPYPSWSATGDAFRHWPIRHASPHQQRPQGQNGWFV
                     SSYNPNQRDAAVGRLRSAPFLIEGELLTIRVAGGRDPEKLRVELLVDGETVASATGNN
                     SEIFARHDWNVSELKGRLGVLQIVDDATDGWGHIMLDELQQWAHRGERRL"
     gene            complement(6573..7214)
                     /locus_tag="VFG22_11185"
     CDS             complement(6573..7214)
                     /locus_tag="VFG22_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416850.1"
                     /translation="MTRTNRNQAQKRHSRVQARARKATVVHDGSAFQLAADLRVYGFE
                     VVEGPLLSSPAAHAEPGSLAVIASRPGAARTERLREWTRQLCWNGTPLLAVGSAVGPV
                     AEFFGSLPIASEEPRVSGRLADIGAQSTGLFTGLPAEFRLALPAGDRFEASKLSTEFG
                     ATAWSQDGELVGASHVFRPIHLLHAGALESGEVRQVVLKNLMRLLRENGGRAF"
     gene            complement(7399..8286)
                     /locus_tag="VFG22_11190"
     CDS             complement(7399..8286)
                     /locus_tag="VFG22_11190"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HET6416851.1"
                     /translation="MSVPAAVLDSPVLYFAYGSNLDDQNWLLWCERKGYDPASIEPLG
                     PAWLPDHELVFHYRSRLRSGGALDVRPRRGTAIPGALFRVHDWKGLDAKEGVSGEYYE
                     RARVTALTADGKAHVATTYRVCDARVGDFVAPGPEYREMVIRGLSRFGHSPEGFLAAA
                     SGNPAPPTPSAIFAYGTLMRGEQSHGLLAPRVRRALGQAQVLGASLVHIDWYPGLVLS
                     EQGSVHGELYELDAIDAALQELDSYEDFVGYESGDSLYRRSLMTAVTSAGPHLAWTYV
                     YLGDASGLRRVSLGRWSGA"
     gene            8313..8930
                     /locus_tag="VFG22_11195"
     CDS             8313..8930
                     /locus_tag="VFG22_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416852.1"
                     /translation="MERDLSGRCGTCGWYIRKREEPDGSWVGDCRLGQGRWPLKDMAT
                     CSSHKSKDTPWEQALKRKPAAGAPRRYRDEPAPKPRPLIPSEIGIDMDQETFRQVLRE
                     VLLDELGVREVELGDRWNEGELVLVPGKEGTQEKRIPLDVFFKKIVMVRDKLRVLEQK
                     INGNKNLSDEEKVHLQQYITGCYGSLTTFNVLFKRKEDQFAGQKK"
     gene            complement(8961..9425)
                     /locus_tag="VFG22_11200"
     CDS             complement(8961..9425)
                     /locus_tag="VFG22_11200"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF59 domain-containing protein"
                     /protein_id="HET6416853.1"
                     /translation="MGDDIERLKIHKIAPRTTSDHVFLPQAGGHAPPVEPVNEAYGGT
                     GETPATPIDPKAVEAAVIDALQTIYDPEIPVNIYELGLIYGVEVNGDGRTEVRMTLTA
                     PACPVAGALVEEVAEKTGRVEGVSTSHVELVWDPPWTPERMTEAAKLELGMI"
     gene            complement(9430..9879)
                     /locus_tag="VFG22_11205"
     CDS             complement(9430..9879)
                     /locus_tag="VFG22_11205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HET6416854.1"
                     /translation="MDLRELYQEVILDHGKNPRNHRFPEPFNREGRGYNPLCGDKITL
                     RLQLDGDTISDVGFIGQGCAISQASCSTMTEAIKGKKLSEVEDLFSKFHTMLTSDKGS
                     DDDRDALGKLAVFAGVREFPMRVKCATLGWHTLNQALRNEDGQASTE"
     gene            complement(9879..11135)
                     /locus_tag="VFG22_11210"
     CDS             complement(9879..11135)
                     /locus_tag="VFG22_11210"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET6416855.1"
                     /translation="MTDLSPAAVSSPFEFDAEQVRNDFPALHQEVHGKPLVYLDSAAT
                     SLKPQSVIDAVVDVYARDCANIHRAVHLLSQRATARYEEAREKVRAFLNAARSREVVF
                     TRGTTESINLVAQSWGRSALREGDEVLVTELEHHSNIVPWQMLCQQTGAKLVVVPMTD
                     RGEILREAFESKLSDRTRLVAMAHVSNALGTVLPVHELIALAHDRGAVVLVDGAQAVS
                     HAPVDVRELGCDFYVFSGHKLYGPSGIGVLYAKEHLLESMPPYQGGGDMIRSVTFDET
                     IYNELPYKFEAGTPNIAGAIGLGAAIDYFTSFDPEAVHAHESALLRYATEALEAIPGV
                     RLIGTAPGKVAVLSFLMDSAHPHDVGTIVDSEGVAIRTGHHCTQPVMDHFGIAATARA
                     SLGMYNTTDDIDALVRALGKVQELFG"
     gene            complement(11132..12349)
                     /gene="sufD"
                     /locus_tag="VFG22_11215"
     CDS             complement(11132..12349)
                     /gene="sufD"
                     /locus_tag="VFG22_11215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HET6416856.1"
                     /translation="MTDASPQTLLGSLPTAFDSGPTWLRALRARAAETLREQGLPTKK
                     TEAWRFTPVRSLVDAAFSSEPESAPELASAPPEGVTVRSLRDVLETEPGLLEGRLDLA
                     GGPTHFAALNTALFADGLWIDVPAGLCVEQPIQLSHAIRASSEPGVTYPRVLVTLGEN
                     AELDLIESYIGGDPKQLTNSVVEVDLGRNAKLSHVRVHQNEGLQVGRVDVRQSADSRY
                     RSTVVTLGGALLRCDVRCLLDGKGAECQLDGVYLLDGEDLVDHHTRVEHRAPHCRSEQ
                     TYRGIIDGKATAVFDGIVVVHRDAQKTEAHQENRNLLLSESATVHTKPHLEIDADDVI
                     CSHGATVGSLDDEQLFYLRARGVPKELAHAMLTYAFLKSIVDRVTHEPTRATLREALL
                     ARIPHAEAIRGIT"
     gene            complement(12346..13098)
                     /gene="sufC"
                     /locus_tag="VFG22_11220"
     CDS             complement(12346..13098)
                     /gene="sufC"
                     /locus_tag="VFG22_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HET6416857.1"
                     /translation="MLKIEDLHVEVGGKPILKGINLEIDAGQVHAIMGPNGSGKSTLS
                     YVLAGREGYEVTKGSVTYKGKDLLEMAPEIRAAEGLFLGFQYPVAIPGVSNIYFLKAA
                     LNAIRKHRGEDPLDAIDFMKLSKEKAALLELPEQLTKRSVNDGFSGGEKKRNEIYQMA
                     MLEPTLAVLDETDSGLDIDALKVVAEGVNALRSPERAMLIITHYQRLLDYIIPDVVHV
                     LIDGQIVRTGDKNLAKQLEKEGYNEVRGTLQP"
     gene            complement(13098..>14039)
                     /gene="sufB"
                     /locus_tag="VFG22_11225"
     CDS             complement(13098..>14039)
                     /gene="sufB"
                     /locus_tag="VFG22_11225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HET6416858.1"
                     /translation="LGSVVPVADNFFAALNSAVFSDGSFVYIPKGVRCPMELSTYFRI
                     NAAKTGQFERTLIIAEEGSYVSYLEGCTAPMRDENQLHAAVVELVAHKDAQIKYSTVQ
                     NWYPGDENGKGGIFNFVTKRGLCEGARSKISWTQVETGSAITWKYPSCILKGDDSVGE
                     FYSVALSNHRQQADTGTKMIHLGKNTKSTIISKGISAGHGQQTYRGGVKISRGATNAR
                     NYTQCDSLLIGDKCGAHTVPYVDVRNGSSKLEHEATTSRIGEDQLFYCRQRGLSEEDA
                     VSLIVNGFAKQVLKELPMEFAVEAQKLLGVSLEGAVG"
BASE COUNT         2701 a         4422 c         4286 g         2630 t
ORIGIN      
        1 gacgccggcg tcggtggcgc tgtcgccgaa gtcatcggcc gcaagattca cgaagtggcc
       61 cagcacagcc aggttctctg catcacccac cttcctcaga tctccgccta cgccgacgcg
      121 cactaccgcg tgcacaaaga agtcgtcggc aaacggacga agagcgacat ccggctgctc
      181 acgaagaacg agcgcttaga agagattgcc cgaatgctgg gcggcgtcac ggtcaccgac
      241 caagcaagag ctgcagccga ggccctgatc caaagcgccg cgacgtagaa agcgcggcca
      301 ctggcaccga cgctgactgg aataccgggg cccgagcgaa cacgacgccg gggtccatcg
      361 aacgccccgg cttgcaattg tggtcgggtg cacttggggc gtttgaccac gcaagccgag
      421 cctcggcgga accccacatc gcgttcgccc gcgcccctcc atatgtcgat ggcagtggta
      481 gagacaggga ctgaagcaac agccggttca cacggaaacg gtcgctcacg tgctcgctcg
      541 ggcgtacatg gagccatgaa ggagggcggc tcgcgatggg cagcacagcg attgggtcga
      601 gcctagccgt aggtcgtctt agagaattgg gggcgagtgg gtcccaatcg cgagcacgcc
      661 cgtcgcgagc cgtcctccgg ggactcagcc gtacaaagga gttgcgggca ctaataactg
      721 acgctgaccc ctcagagctg cttcgggccc acgagcgtgc ggagcgtccc ctcgaccacg
      781 atgtcaccgc cctcgtcccg aagggtcacc ttcacttcgt actcccgctt cgcgctggtc
      841 tcgaccggag acatgcggca ttcgcccgta atcgtgccgc gggccttctt gatgtagtcg
      901 aggctcatcc cggccacgat gaaacgggcg tcgtccggaa gagagtaacc gagcgccagg
      961 ttgcctgtga gctcgacgag gttggcaagg gcgaccgcgt gcacgcagga aaggtgattt
     1021 cgaaccgacc gacggtcggc aagggtgacg cgtgaatagc cgtctccgag ctcctgaacc
     1081 atcgcgccaa tcgacccggt gtacggcgcc aggcgcccaa ccaacttgct gtagagaagc
     1141 tttccacctg gaactcgttc caatcgattc cagacctcac ggacgaaatt tgtcttggat
     1201 tccgtggtga gcccttcggc tctcaaaaac gtggtgactc ccattagacg aaccgtaaca
     1261 cagaggggcc gtgcgcatat cccaactaca acctctcctt catttgatcc ccctgcctgg
     1321 gtccgaaagc ctcaccaact ggcgggctcc aggcagggac ttttaacttc cgccatgccc
     1381 tgcaaatcgg ctgcgggtct ctatttgcta agcaggtcgc cgagccgggt caggctttcg
     1441 tgggcgccac cgagcaagag ctccaggtgc ttcgaggtca tgaagtagcg gtagagtggg
     1501 tagtccctat cgaagcccat gccgccgtgc acgtgctgag ctgccgcaac gacacgcgcc
     1561 cccgcttcgg aggcgaagta tttggcgacc gcaagctctt tgtccgcgtt gagtccctga
     1621 tcgaggcgcc agcaggcttg ccacatcgac gagcgcattg cctgaacgtc gatgtaagca
     1681 tcgcccaccc tctgggtgac gccctgaaaa gtcgcgatcg gaaccctaaa ctgctttcgc
     1741 tcgcccgaat acttggcggt catgaacagc gcttgctccg caatgccatg ctcgacggcg
     1801 cagatcgcga gattggtccg atcgaccagg aaccgcagca cattcgagcc ttcattcgcc
     1861 gagcccaagc gatcggacgc cgctacggcc gcgtcggaga gctcgagaaa ccagagcggc
     1921 tggccattcg tcccccgctg tgcgcccaga cgaacccctt tcgccttggg atcgacgaga
     1981 aagacgccaa tctcgcccgc ttcggtccgc gcgggcacga ggatgcgggc ggcgagatct
     2041 gcgtacgcca cgttgctgaa gcgccccgtc agcttccaac cttcacgctc ggtccgggca
     2101 agcgcggaag gttcgagcgg gttgcggctg cccgagtcga ccagcgccgc cgtcaggatc
     2161 gtctcaccct cggcgatgtc ggggagaaaa cgtcgctttt gctcatcggt gccgaacttc
     2221 gcgatcggca gcgcactcga cgccagcgtt tcgaccgccg gaatcggcgc caccgtgcgc
     2281 ccgacctgct gcaagagaat gcagagctcc gtgagcccca tcccggcgcc gccgtactcc
     2341 tcgggaatcg ccaaaccaag catctccgat tccgcgagct gcttccatgc acgctcgtgg
     2401 aaccagatgc tctctttggc gagcgccttg agcgaatcgt gggtgacgat gtcggaaagc
     2461 acctgcttga cgagctcgcg aatgctgttc tggtcttcgg agaatgagaa atccatgtgg
     2521 tgcctcgtca gcgacgcccg agccgcggca tacgcagccc tgcctggcaa atgatgtcgc
     2581 gttgaatctc gttggtgcca ccgccgaacg tgaggaccag cgacatgcga tagtatttct
     2641 ccaagcgccc gcggacgatc gctccttcgg agctgtggtg gagcgcgccc gcggctccat
     2701 ggacttccat gagcagccgc gacccttcga cgtaaaactc ggagccaaac actttgatcg
     2761 ccgaagcgtc tgccggcgat agcgcgtcct ttgcgatgcc ccacgcctgc tgccagttca
     2821 gcagcttgag cacttcgagc ttcgcgtata cgcgcgcgag gttggtcttc acccagggta
     2881 ggtcgatgac cataccgccc tcgccgttgg gcgtcttctt ggcccattcg atggtctccg
     2941 tgcagatctt ttcgaggggg cccacggaac aaagagcgac gcgttcgtga ttgagttgag
     3001 tggtgatcag atgccagccg ttgttctcgc cacccacgat gttgccgatg ggaacccgaa
     3061 cgttgtcgta gtagaccgca tgaacgttgt tctctccgag cgcatgcatc ggcgtgatct
     3121 gaacgccctc ggcatctgct ggcacgatga tgatcgaaat ccccttgtgc ggcggcgcat
     3181 cgggatcggt tcgacaggca agccacatgt agtcagcgtg gtctgcaagg ctggtccaaa
     3241 tcttctgacc gttgatgacg tactggttgc cgtcgcgtac ggcgctcgtc tgtagcgacg
     3301 ccaggtccgt gcccgagccc ggctcggtgt acccgatcga aaaatgacac ctcccctcga
     3361 ggatcttcgg ggggtattcg gccttctgct cctcggttcc gaacttgagc aaggtgggcc
     3421 ctaccgtgtt caacgtgaga atcggcacag gaaagcccgc gcgctgcacc tcgtcgaaga
     3481 agatgaattg ttcgatgggg ccccggccct ggccgccatg ctccttgggc caaccgatcc
     3541 cgagccagcc atcagctccg agtttctgca tcgcctcgtg gtaaaggggg ccgccacctt
     3601 cggcttcgtc gagctcggcg agaagctcct cggtgatcaa ggtactgaaa taggcacgca
     3661 gctcgtcgcg cagtgccttt tgctcggggg tgtaatcgaa atacatggcc tcagggacgc
     3721 cggcccagtc gccggagtga aacgtgttct agttacccct cgccgcggtc gcaagccgaa
     3781 ccggtccgca gacgcaaaaa agccggctcg tgtgagccgg ctcatttcac gatatcgttc
     3841 tgagatctcc gctcagtatt tcttcttggc tgcgtcggcg ccagccttca ccttggcttc
     3901 ggcctcgtcg gctgcggcct tcacgtcgtc tgcggctcct tcggcagctt cctgagcttc
     3961 ctcggcggct ccttcgacag cttcctgagc ttcctcggcg gcctcgccag ctgcctccat
     4021 ggcttcgtcg gccgcctcaa cggcaacttc agcagcctcg gcggctccct cggcagcctc
     4081 ttcagcctga tgtgctgcat gatccgctgc ttcagcggcc tcgccagctg catcttcggc
     4141 cttcttacag ccgatcattg ccccgcagat cgcgaggacc agacccaatc gaaaaatatg
     4201 tttggtcatt agggattctc ctccgttggt aaaacgcggg gcacaccact gcacccacac
     4261 tccccgctcc gaggggggag ttagcgtttt gaagccatcg cgtcaaaccc ggaacccaca
     4321 ccagcgcacc tggagcgaag gttggtccct tgcgtgccga ccaagtggtg agccgcgaat
     4381 gaagccgtaa ctttgcgcaa gcaaaggagg aagccgagtg ttggggctct ccgccaaatt
     4441 cagtgagagg cgactattac ggtggaaatg gtcatccggc gcaagttgtc attttctgtc
     4501 agagacgccg ctcaccgcga tgcgcccact gttggagctc atcgagcatg atgtggcccc
     4561 aaccgtcagt cgcatcatcc acgatctgaa gcactccgag ccggcccttc agctcggaga
     4621 cgttccagtc gtggcgcgcg aaaatctcgc tgttgttgcc cgtcgcagac gcgacggttt
     4681 cgccgtcgac cagtagctcg acccttagct tctccggatc acgaccgccg gccacacgga
     4741 tggtgaggag ctcgccctcg atgagaaagg gggcggaacg aagtcgcccc accgcggcgt
     4801 cgcgctgatt cggattatag ctcgagacga accatccgtt ctggccttgc ggcctttgct
     4861 ggtgtggaga tgcatgtcga attggccagt gccgaaacgc gtcgccagtc gcggaccagc
     4921 tcgggtacgg aacggcctcg aacgaaatgc cggcgacctc ggtccagccg ttgtcctcgt
     4981 tcaattcgtc tcgggtccag atgccaggct catcgagctc tgctgggatg ttggcgtcga
     5041 ggtagtaacc gtagcggtag agatcgcgac ggatgtatcc gcgcttgatg tacgtggggt
     5101 gcttcgaaag caggtagtcg gggtctgcgc gcttttcgtg tccagccttc ccacgaccga
     5161 agcgcgtctg atcctgatgc gcagtgatgg ggtcgatgac gccgaagaag tcgtagacat
     5221 acaggttcgc gtagaagccg ctcgacccaa tgtcgcccgt tacgatggac gcgcctggcg
     5281 gcgcttgcgc gccgagccac tccatcaatc cccggacacg ggcgacctca tgaccccagc
     5341 gcgtgatctc cgtctgaaca ggaccgactc gatagccacg aacggcaacc atcgcgagca
     5401 atgccgcgcc aagcgcgtag accgcccatg agagtcgcgg ggcagagctc gcacgagctc
     5461 ggacgaagga ggcggcgcgc tccaaggtgt atgcggacag caaggcggtt accggaatca
     5521 gaaccacgag gaaacggtgt cccgggtaga aatcccctcc aacgtagacg acatacgctg
     5581 aatagaggag tgcccaagcc agcagcggcc cgatcacccg gtcccgaatc ccaagcaccg
     5641 cccccagcga gagcaaaggc acgtaggcgc ggtcccggag gaaatcatcg aggtaccgaa
     5701 gaccccgttc gatcgcttcg ccacggagac cggagacttt tacgtagaac gtgttcggta
     5761 ggaggtaacc gaagtactga taccgccacc caaaatagac gccgaagacg acggccgtcg
     5821 aaccgaagat cgcgattgct cgcaacagcg caggccgccg ccgctcggat tgaaaagcga
     5881 acaccaactc gctcacaccg agcaccgcaa cgaacaatgc cgcatcgggc cgagtcatca
     5941 ttgcggccgc gaggctgagc gcggcgccaa ttgcgctcca atggcttggc gtgccccaaa
     6001 gaaacaaaca agcggcgagc atcgcaaaaa tgagcagatg ggattcgagc gcctgcatgg
     6061 cccaaacggt gtatccattg tcgaacacac aaagtccgag catgagcacg atcggcacga
     6121 gccgtcgggg ccataccaaa cgtccgaggc gatagagcaa gacgatgtcg agcgcggcca
     6181 gaagaacgct gagcgcagtt gcgaggtgta cgaaggctgc gctgctgcca cgactcagag
     6241 ggtgtgccag tgccaagagc gaaacccaga gaaaattggt gaagccctcg acacgttcgc
     6301 ccgagttgaa cacgaagccc agtccttggg cgacattgcg cgcgtattgc aaggagatga
     6361 gcgaatcgtc gcagacgttg tacttgtatc ggcgcgacca ttcgaaagcg agcgctgcaa
     6421 ccacgacggc gccgccgatc gcccaccaca gggcacggtc gcgctggggc cgagaaggct
     6481 ggccttccac cccgacttcc ggaaccgccc gagcctcagg catgcgcaat agctagcacg
     6541 tctagaccag aaccgcgctg gcactcgcgc tgctaaaatg cccgtccgcc gttctccctc
     6601 agcaagcgca tgaggttctt cagaaccact tgtcgcacct cgccgctttc gagcgctccg
     6661 gcgtgcaaca agtggattgg gcgaaacacg tgagacgcgc ccacgagctc gccatcctga
     6721 ctccaggccg tcgcgccgaa ctcggtgctc agcttggacg cttcgaaacg atctcctgcc
     6781 ggtagcgcga gccgaaactc agctggcagg cccgtaaaca ggcctgtgct ctgagcaccg
     6841 atatccgcca agcgcccgct cacacgcggc tcctctgaag caatgggcaa ggaaccgaag
     6901 aactcggcaa ccggacccac cgcggaaccc actgcgagga gaggtgtccc gttccagcaa
     6961 agctggcgcg tccactctcg aaggcgctcg gttcgggcag ccccgggccg cgacgcgatc
     7021 acggccaggc tcccgggctc tgcatgcgcg gccggcgatg agagcagcgg cccttctacg
     7081 acctcgaatc cgtagacacg caggtccgcc gcgagctgga acgcggatcc atcatggacg
     7141 acggttgcct tgcgcgcgcg agcttgtacg cgcgagtgtc tcttctgtgc ttggtttcgg
     7201 ttggtccgtg tcatgggtcg ggctcccagc ccctcccgga cgacctcaga ttctaaaaac
     7261 caagaaggcc gccctgagag gcggcctttg agcgacaaaa cgcgcaggcc acctcagtcg
     7321 gtgtaccaaa agtagttacg cagaatgtcg aagtgagtct tcatctcaat tgagagttta
     7381 cggctctccc caggggcgtc aagcgccgga ccagcgtcct aaagacactc gccggagccc
     7441 gctcgcatca cccaaataga cgtatgtcca ggcgagatga ggcccggcgc tcgtcacagc
     7501 ggtcatcaag gaacgccggt agagggagtc tccgctctcg taccccacga agtcctcgta
     7561 gctgtcgagc tcttgcaaag ccgcatcgat cgcgtcgagc tcgtaaagct cgccatgcac
     7621 cgaaccctgc tcgctcagca cgagccccgg ataccaatcg atatgaacga gcgatgcacc
     7681 caggacctgc gcctggccga gagcgcggcg tacgcgcggt gccaataggc catgactctg
     7741 ctctccgcgc atcaaggtgc cgtaggcgaa gatcgcggac ggggttggtg gagccgggtt
     7801 cccactcgct gcagccagaa aaccctcggg ggaatgcccg aaacgcgata ggccgcggat
     7861 caccatctct cggtactcgg gtccgggcgc gacgaagtca cccaccctgg cgtcgcaaac
     7921 gcgataggtc gtcgccacat gagctttgcc gtcggctgtc aacgcggtca cgcgagctcg
     7981 ctcatagtac tcacccgaaa ccccctcttt ggcgtcgagc cctttccaat catgcacccg
     8041 aaagagagcc ccagggatcg cggtgccgcg tcggggccgg acgtcgagcg ctccgccact
     8101 gcggagtcgc gaccgatagt gaaaaacgag ctcgtgatcg ggcagccagg caggtccaag
     8161 cggctcgatc gacgcagggt cgtatccctt gcgctcgcac caaagcaacc agttctggtc
     8221 gtcgagattg gagccgtagg cgaagtagag cacgggtgag tctagcactg ccgctggcac
     8281 cgacaccgct cctgacctaa cctccggcgc gaatggagcg ggacctttca ggccgatgcg
     8341 gcacctgtgg ctggtatatc cgcaagcgtg aagagcccga tggttcgtgg gtcggtgact
     8401 gcaggctcgg acaagggcgc tggccgctca aagacatggc gacgtgctcc agtcacaagt
     8461 ccaaggacac gccctgggaa caggcgctca aacgaaaacc cgcagcgggt gcgccgcgcc
     8521 gctaccggga cgagcccgcg cccaagccgc gcccgctcat tccttcggag attggaatcg
     8581 acatggatca agaaactttc cgccaagtcc ttcgagaggt gcttctcgac gagctcggag
     8641 ttcgcgaggt cgagcttgga gatcgctgga acgagggcga gctcgtgctc gttcccggca
     8701 aagagggaac ccaggagaag cgcattccac tggacgtctt cttcaagaag atcgtcatgg
     8761 ttcgcgataa gctccgcgtg ctcgagcaga agatcaacgg caacaagaac ctgagcgatg
     8821 aagagaaggt tcacctccag cagtacatca cgggttgcta cggttcgctg acgaccttca
     8881 acgtcctgtt caagcgcaag gaggatcagt tcgccgggca gaagaaatag tctgccgcag
     8941 ggtccgcgac ctcgagggct ctagatcatg ccgagctcga gcttggccgc ttccgtcatg
     9001 cgctcagggg tccatggagg gtcccaaacc aactcgacgt gagacgttga aacgccttcg
     9061 acgcggcccg tcttctccgc gacttcttcg acgagcgcgc ccgcaacggg gcaggccggg
     9121 gccgtcaacg tcatgcgcac ttccgtgcgt ccgtccccgt tgacttcaac gccatagatc
     9181 aggccgagct cgtagatgtt caccggaatc tccgggtcgt agattgtctg gagtgcgtcg
     9241 atgaccgcgg cttcgactgc tttgggatca atgggtgtgg ccggtgtctc cccggttccg
     9301 ccgtacgcct cgttgacggg ttcgacgggc ggagcgtggc cgccggcttg cggcaagaag
     9361 acgtggtccg acgtggttcg gggcgcgatc ttgtggatct tcaagcgttc gatatcgtcg
     9421 cccaccgctc tactccgtgc tcgcttggcc gtcttcgttg cgaagggctt ggttgagggt
     9481 gtgccagcca agggtggcgc atttgacgcg catcgggaac tcgcggacgc cagcgaacac
     9541 cgcgagcttg ccgagcgcgt cgcgatcatc gtccgagcct ttgtcgctgg tcagcatcgt
     9601 gtgaaacttc gagaacaaat cctcgacctc ggagagcttc ttgcccttga tcgcctcggt
     9661 catcgtggag caggacgctt gcgagatcgc gcatccttgt ccgatgaagc cgacgtcgct
     9721 gatcgtgtcg ccgtccagct gcaagcgaag ggtgatcttg tcaccgcaca gggggttgta
     9781 gccgcgtcct tctcgattga atggctcggg gaaccgatgg ttcctcggat tctttccgtg
     9841 atcgaggatc acttcttggt agagctcgcg aagatccatc agccgaacag ctcctgcact
     9901 ttgccgagcg cgcggaccag ggcatcgatg tcgtcggtcg tgttgtacat accgagcgaa
     9961 gcccgagcgg tggcggcaat gccgaaatgg tccatcaccg gctgcgtgca atggtgcccc
    10021 gtacgaatcg cgacgccttc cgaatcgacg atggtcccca cgtcgtgtgg atgcgctgaa
    10081 tccatcagaa acgagagcac cgcgactttg ccgggcgccg tccctatcag tcgcactccg
    10141 ggaatcgcct cgagcgcctc ggtcgcatag cggagcagcg cactctcgtg ggcgtgaacg
    10201 gcctctgggt cgaacgaagt gaagtagtcg atcgcagcgc cgaggccgat ggcgcccgcg
    10261 atgttgggcg tccccgcttc gaacttgtat gggagctcgt tgtagatcgt ctcgtcgaag
    10321 gtgaccgagc gaatcatgtc gccgccaccc tggtagggcg gcatgctttc gagcaggtgc
    10381 tccttggcgt agagaacgcc gatgccgctc ggtccgtaga gcttgtgccc gctaaacaca
    10441 taaaaatcac agccgagctc acgcacgtcg accggggcgt gggagacagc ctgcgcacca
    10501 tcgactaaga cgacggcgcc ccgatcatgc gcgagcgcaa tcagctcgtg aaccggtagc
    10561 accgtcccaa gggcgttcga tacgtgcgcc atcgcaacca gccgcgttcg gtcactgagc
    10621 ttgctttcga aggcctcgcg aagaatctct ccgcggtccg tcatcggcac gacgacgagc
    10681 ttcgcgccag tctgttggca gagcatctgc caggggacga tgttggaatg gtgctcgagc
    10741 tccgttacga gcacctcgtc tccttcgcgg agcgcgcttc gcccccagct ttgtgctacc
    10801 agattgatcg actcagtcgt cccgcgggtg aagacgactt cacgacttcg cgcagcgttc
    10861 aagaacgcac ggaccttttc acgagcctct tcgtatcgag cggttgcgcg ctgcgagagg
    10921 aggtgaaccg ctcgatgaat gttggcgcag tcgcgagcat acacgtccac aaccgcgtcg
    10981 atcaccgact ggggcttgag cgacgttgcc gcgctgtcga gatagacgag cggcttgcca
    11041 tgcacctcct ggtgcagcgc agggaagtcg ttgcggactt gctcggcatc gaattcgaac
    11101 ggactcgaaa cggctgccgg cgagaggtcc gtcatgtgat cccccgaatg gcttcagcgt
    11161 gaggaatccg cgcgagcagc gcctcgcgca gggttgcgcg cgtcggctcg tgcgtcacgc
    11221 ggtcgacgat cgactttagg aacgcgtacg tgagcatggc gtgggcgagc tccttcggaa
    11281 cgcctcgcgc acggaggtag aagagctgct cgtcgtcgag cgagcccacg gtcgccccgt
    11341 gactgcaaat cacgtcgtcc gcgtcgatct cgaggtgcgg tttggtgtgc actgtggccg
    11401 actcagagag aaggaggttg cggttctcct ggtgggcctc ggtcttctgg gcatctcgat
    11461 ggacgaccac gatgccatcg aaaaccgcag tcgcctttcc gtcgatgatg ccccgataag
    11521 tctgttcgct tcggcaatgc ggtgcgcgat gctcgacacg cgtgtggtga tcgaccaggt
    11581 cctcgccgtc gagcagatag acgccatcga gctggcattc tgcgcccttg ccatcgagca
    11641 agcagcggac atcgcatctc aaaagtgcac cacccaaagt gaccacggtg gagcgatacc
    11701 ggctgtccgc gctttgacgc acgtcgacac ggccaacctg aagtccttcg ttctggtgta
    11761 cgcgcacatg gctcagcttg gcattgcggc cgaggtcgac ctcgacgaca gagttggtga
    11821 gctgcttcgg gtctccgccg atgtagctct cgatcaggtc gagctcggcg ttctccccca
    11881 gcgtgacgag gactcttgga taggtgaccc ccggctcaga tgaagcgcga attgcatgag
    11941 atagctggat gggctgctcg acgcagagcc cggcagggac atcgatccaa aggccatcgg
    12001 cgaagagcgc ggtgttgagc gccgcgaaat gcgtcggtcc cccagcgaga tcgagacggc
    12061 cctcgagcaa accgggctca gtctcgagca cgtcgcgcag gctcctcacc gtgaccccct
    12121 cgggaggcgc gctcgccagc tctggggcgc tctccggttc ggacgagaac gcggcatcga
    12181 ccagcgatct cacgggtgtg aatcgccagg cttcggtttt cttcgttgga agcccttgct
    12241 ctcgaagtgt ttcggccgcg cgtgcgcgaa gcgcgcgaag ccaggtcggt cccgaatcga
    12301 acgcagtcgg aagactcccg aggagtgtct gtgggctcgc gtcggtcacg gctgaagcgt
    12361 gccccgaacc tcgttgtaac cctctttttc gagctgcttg gcgaggttct tgtctccggt
    12421 tcgtacgatc tggccatcga tgagcacgtg gacgacatcg ggaatgatgt agtcgagcag
    12481 gcgctggtag tgggtgatga tcagcatggc gcgctcgggg ctccgcaatg cgttgacgcc
    12541 ctcagcgacc accttgagcg catcgatgtc gaggcccgag tcggtctcgt cgagcaccgc
    12601 gagggtgggc tcgagcatcg ccatctggta gatctcgttt cgctttttct cgccgccgct
    12661 gaacccgtcg ttcacggaac gcttggtgag ttgctcagga agctctagca gcgcggcctt
    12721 ctccttcgag agcttcatga aatcgatcgc atccaaggga tcttcgcctc ggtgcttgcg
    12781 aatcgcgttg agtgccgcct tgaggaagta gatgttgctc acgccgggga tcgcgacagg
    12841 gtactgaaat ccgagaaaca gcccctctgc ggccctgatt tcgggcgcca tttccaagag
    12901 atccttgccc ttgtaggtga cgctgccctt ggtcacctca tatccctcgc gaccggcgag
    12961 cacatacgag agcgtgctct tgccggagcc gttgggcccc atgatcgcgt gcacttgccc
    13021 cgcatcgatc tcgaggttga tccccttgag gatgggtttg ccgcctactt cgacgtgcag
    13081 gtcctcgatc ttcaacatca accgactgct ccttccaagc tgacgccgag gagcttctgc
    13141 gcctcgaccg caaattccat gggcagctct ttcaagacct gctttgcgaa gccattgacg
    13201 atcaggctca ccgcgtcctc ttccgaaaga ccgcgctgcc ggcagtagaa gagctggtcc
    13261 tcaccgatgc gcgaggtcgt ggcctcgtgc tcgagcttcg aggagccatt gcgcacgtcg
    13321 acatagggca ccgtatgcgc accgcatttg tcgccgatta gaagcgaatc gcactgtgtg
    13381 tagttgcggg cgttcgtggc gccgcgggaa atcttcacgc cgccgcgata ggtctgctgc
    13441 ccgtgccctg cgctgattcc cttggaaatg atggtcgact tcgtgttctt gccgaggtga
    13501 atcatcttcg tgcccgtgtc ggcctgctga cggtgattgc tcagcgccac cgagtaaaac
    13561 tcacccacgg aatcatcgcc tttgagaatg cagctcgggt atttccaagt gatcgctgac
    13621 ccggtctcga cctgagtcca gctgatcttc gagcgcgcgc cttcgcaaag gccacgcttg
    13681 gtgacgaagt tgaagatgcc acccttcccg ttctcgtcgc cggggtacca gttctgcacc
    13741 gtggaatatt tgatctgagc gtctttgtgg gcgaccagct cgacgaccgc ggcatggagc
    13801 tggttctcgt cacgcatcgg cgccgtgcag ccctcgaggt agctcacata gctaccctct
    13861 tcggcgatga tcagcgttct ctcgaactga cccgtctttg ccgcgttgat tcggaagtaa
    13921 gtggaaagct ccatcgggca tcgcacgccc ttcgggatgt agacgaacga accgtcgctg
    13981 aagaccgcgc tgttgagcgc tgcaaagaag ttgtccgcga cgggaacgac cgagccgag
//
LOCUS       DASRZF010000254        14804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4654854, whole genome shotgun sequence.
ACCESSION   DASRZF010000254 DASRZF010000000
VERSION     DASRZF010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 14804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14804
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4654854"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VFG22_11230"
     CDS             complement(<1..263)
                     /locus_tag="VFG22_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET6416859.1"
                     /translation="MAKEKFVRNKPHINVGTIGHIDHGKTTLTAAIMKVMSDKHGGEV
                     KSYKDIAKGGTERDDTKTVTIATSHVEYQTGNRHYAHVDCPGHA"
     gene            complement(275..2389)
                     /gene="fusA"
                     /locus_tag="VFG22_11235"
     CDS             complement(275..2389)
                     /gene="fusA"
                     /locus_tag="VFG22_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET6416860.1"
                     /translation="MARKFPLEKTRNIGIMAHIDAGKTTTTERILYYTGVNYKIGEVH
                     DGAATMDYMAQEQERGITITSAATTCFWRPGVGPFGSDQFRINIIDTPGHVDFTIEVE
                     RSLRVLDGAVAVFDGKEGVEPQSETVWRQADRYRVPRICFINKMDKAGADFPTAFQSI
                     KDRLGAPAVAVQLPLGQEAELQGVIDLVAMKANRFTEDKLGAEWIVEEIPDEFRADAE
                     KARAALLEAASEVDDALMERYLEGDTDFSEEEIILAIRKGCLAQTIVPVLTGSAFKNK
                     GVQQLLDAVVNYLPSPLDVPAIQGVDPDDPEKKMERKASDDEPFSALAFKIINDPFVG
                     QLTFFRVYSGMLEAGSGVLNSTKGKKERVGRILQMHANNREELKEIHTGNIAAAVGLK
                     TATTGDTLCDDKAPIILERMDFPEPVISVAIEPRTKADQDKLGVGLGKLAAEDPSFRA
                     HTDEESGQTIISGMGELHLEIIVDRLKREFAVDSNVGAPQVAYRETITKSVESEGRYI
                     KQSGGRGMFGHVWVKLEPMEAGSGFEFENKIIGGVIPKEFIPAVQKGIKDAMSRGVMA
                     GYPVIDVKATLFDGSYHDVDSSQAAFEVAGSLAFQEGAKRAGIQLLEPVMDVEVRVPE
                     DYMGEVIGDLNSRRGNVTGMDSVSGIQVIRSFVPLANMFGYVNDLRSKTQGRAVYTMQ
                     FKEYQGVPQGIADEIVAKAKGG"
     gene            complement(2624..3094)
                     /gene="rpsG"
                     /locus_tag="VFG22_11240"
     CDS             complement(2624..3094)
                     /gene="rpsG"
                     /locus_tag="VFG22_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HET6416861.1"
                     /translation="MSRRVSAPKRHIIPDAKYGDQLVAKFVNSLMLAGKRSTAEKCLY
                     GAFNIIEERYKDEPLDVFKKALDAVKPRVEVKSRRVGGATYQVPVEVRSDRRNALAMR
                     WLVQYSRARGEKSMEERLAAELMEASQGRGNAVKKREDTHKMADANKAFAHYRW"
     gene            complement(3108..3506)
                     /gene="rpsL"
                     /locus_tag="VFG22_11245"
     CDS             complement(3108..3506)
                     /gene="rpsL"
                     /locus_tag="VFG22_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HET6416862.1"
                     /translation="MPTINQLVRKGRKQIKSKTDSPALQRCPQKRGVCVRVYTTTPKK
                     PNSALRKVARVRLSNGIEATTYIPGEGHNLQEHSVVLIRGGRVKDLPGVRYHVVRGTL
                     DATGAAGPSNTNKANRVNKRSKYGVKRPKN"
     gene            complement(3739..4650)
                     /locus_tag="VFG22_11250"
     CDS             complement(3739..4650)
                     /locus_tag="VFG22_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416863.1"
                     /translation="MRRAWLSGLLVLSMAVYSLPVAADELSDFESARRNYDKQNYSKA
                     ARGLESLVGGAVPRASNSVVRLEARKYLGATYLFLGKDEAAREQFRALLEEDPAYDID
                     PVAFPEAVVQTFQEVQTEVAAERARQEALSAARKQQERTEELEALIRQQERIAALEEL
                     ASTETVEKVNSRWIAALPYGIGQFQNESRKLGIMFAVTESAFTVAAIATFIGHNSLRN
                     ENPAPSEIERAQRVERALRIGNWVSVGALVGFGLAGVIEAEVRFKPVIRSTRKRDLRP
                     DAESAESKGPQIRLNLGLTGGNFRLDF"
     gene            complement(4666..6486)
                     /locus_tag="VFG22_11255"
     CDS             complement(4666..6486)
                     /locus_tag="VFG22_11255"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6416864.1"
                     /translation="MLRKLEKYEVLDEIGHGGMATVYRARDSSLDRFVALKVLHPHLQ
                     RTSEARARFTREAKSVAKLRHPHILEIYDYSGESSDETYIAAELLTGPTVKEFVLRQK
                     DLPPEIAAAIALQLAEALSEAHAKGIIHRDVKPENVMIHEDRCVKLTDFGIAHMVDTH
                     TFTATGQILGSPGHMAPEQVEGGTCDVRSDVFSLGTVLYFCATGRLPFIGRNTHHLLK
                     LLLEGEYPDALRVRPEIGADLAAIIDKALSRRPADRYQSASAMAEDLRAFLAAMGVEQ
                     PDETLARYLASPDDVGTEIRENSLQRLLAIGASSAKAGDVLNAQAALSRALAIDDGNE
                     RALKLLGSLGRRRLRNALIFLVAAILLLSLGGAAYVAWLLEQGAVRPDKSLPVAADPG
                     SESQGDFGGKANEPAPPPPADPVDGSKTSSGRNAESEDLDPGSSERDGEQSAASNAPR
                     WVVFKPTPPNVSISVDGSPLRAYGPDYQGVRLKVGSHTFRFVGAEDCCEQRVVRKRIP
                     PGTRDFELPVKLRFKPATLYLKGPTQVDATVQITLPGGRKVSGRIREIFRIPMRSLQT
                     SAEVKIRAPGYRTYKSVVQLRAGGDLVEHAFTLEREGETP"
     gene            complement(6538..7812)
                     /locus_tag="VFG22_11260"
     CDS             complement(6538..7812)
                     /locus_tag="VFG22_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416865.1"
                     /translation="MFEAVIVDGEGGNPAAGTDATTLRVGIQEGELAPVEYEYPITEG
                     DFEGVLEFRVFAAPTRVRVEIEGATTELLTAPPAFVPGASAGFMRVVTAAPSSCERVL
                     LNSMEAPRAFFGMVQSGTFALLVGGASSTDAQVEYLDVLEWKSHLFEEEFSLSELGET
                     RAASIDEGKILVLPSNASPFIFDMFDPTRRITAVILHNGAGTRSALVSVPGLGAMVIG
                     GEVSGVPQSTVSLVEPDGTVVSLRLSVPRSDPQATALGMDVLVAGGDDDGTAEILLEG
                     EQVGQSVAGVMDGVRKAGLLVGDGENRALWMGGVDADAALRQDTLRFDDCPSGCTASA
                     GPAWPTARLAALQPQRSQLVIGGEGSSRVDEVRWSGTDVEISPLLDLNVPRAGAGGIV
                     LESGAFIVAGGDDGVSVRDDFEFCVPAALEPL"
     gene            7936..9258
                     /locus_tag="VFG22_11265"
     CDS             7936..9258
                     /locus_tag="VFG22_11265"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HET6416866.1"
                     /translation="MWAEDVASIEELAVARRLSAEPGFAWLDSNRSEARDGRYSFIAA
                     WPCDEIRVEFGAEAPFSALRGLSRPEAVVNDQGPKPCEVPAWIGYIAYDAAWCAPPRG
                     EPRFEREREPVLLFRRYSALIAIDHEQNKAWIVGDDQNACDEFLGRLRGLVDREGSLT
                     KIGPVEVDDAASHQNAIERALDYISMGEVYEVNVARQWSAAFEGDALALWTAMRIASR
                     VPLGMYLDAGDHSVLACTMERFIHWDAESKTLVTRPIKGTIRREATDDGASERLRADP
                     KEQAEHSMIVDLMRNDLGRVAETGTVKVDAPLIVEPFTGLYHLVSTVSCVTRPDVSLE
                     SILEATFPPGSVTGAPKVRAVEIIEELEEHPRGIYCGAIGFIDHDGGISLAVAIRTAT
                     VAKGKVTYWAGGGIVEASDPGREVEETELKARVFLDAIESLRRASGDE"
     gene            9251..9853
                     /locus_tag="VFG22_11270"
     CDS             9251..9853
                     /locus_tag="VFG22_11270"
                     /inference="COORDINATES: protein motif:HMM:NF017819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HET6416867.1"
                     /translation="MSERIQDILDFWFGELDEHGCASPNQRKRWWAKSDAFDETVKAQ
                     FLDDYEAVVDGEREAWRNTAHGALAYIIVLDQFSRNMFRGTSKMFEADELARDVCYEG
                     LDSGFDMELGFDERVFFYLPLEHSESMDDQMRCLDMFETLAEDAPEHLQVDARNYLDF
                     ANRHRAIIERFGRYPHRNDALGRASTPEEREFLETPGSSF"
     gene            complement(9850..10980)
                     /locus_tag="VFG22_11275"
     CDS             complement(9850..10980)
                     /locus_tag="VFG22_11275"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6416868.1"
                     /translation="MNRMFAIAWKEIIQARRDRLTMGMMIGIPIIQLLLFGYAINTDV
                     RNIATVVYDQDHSEESRGFVRRMEATRFYEVVGYVESYEQIGVALRSRQAMVALVVPP
                     RFGAEVEAGRMAQLQLVVDGSDPQVVASATNTAMSLAAARSSALTVERLQRLGQPASI
                     SSQVIALEPTTWYNPDLRTAVFIVPGLVGVILTMTLVMFTAMAIARERERGTLEQLIV
                     SPIRSGELTIGKILPYIAVGYLQMSLILVVGSWVFAVPIVGSVALLYGLSVFFIAASL
                     AVGLFFSTLAKTQQQAMQMSFFFLLPNILLSGFMFPWEAMPAPARWLSMMLPLTHYLR
                     VIRGIVLKGASFMELRSELVWLAGLTLFLTIVVSARFKKKLD"
     gene            complement(10977..11915)
                     /locus_tag="VFG22_11280"
     CDS             complement(10977..11915)
                     /locus_tag="VFG22_11280"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6416869.1"
                     /translation="MDDPVVVEGLSRHFGKLLAVSDVSFRVHRGEIFGLLGPNGAGKS
                     TTLRMLCGILDPSAGRATVVGFDVASQPERVKQNIGYMTQRFSLYEDLTVEENLRFYA
                     GIYGLGRAERRVRVPEELVRSGLDDRRHQIAGTLSGGWKQRLALVCATIHQPPLLFLD
                     EPTAGVDPVSRREFWAHIHRIAAGGTTVMVTTHYMDEAERCHRLAFIFRGTLLDLGTP
                     SEVIERREIRIAIISTSDAAAAMDLLTSHPSVDEVAPFGDSLRVATLGGVDPVALSHE
                     VLGGGPGVRDAREIVPNVEDAFVAMVHSEARSGSSV"
     gene            complement(11918..12895)
                     /locus_tag="VFG22_11285"
     CDS             complement(11918..12895)
                     /locus_tag="VFG22_11285"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HET6416870.1"
                     /translation="MTTLVRAGLLMGAIIIVTLCGCDRERAMPAGYQGIVEHDDRHLG
                     FRVGGQLREIRFDRGDIIEEGAVIAILDDTAELPILLAREAELARARANAALVYAGPR
                     VEEVRSTMAELDGARAALHEARANLDRHRNLPKEAATARSLVSELEAAYAQSKAQVRV
                     LQERLKQLRIGARPEELAAVDAEVTAAEAAVEAERERLALYSMEAAERVTVLDVPVRV
                     GEVVAAGATVATVADTTHPYVDVFVPQQALTGLRVGLPANVRVDEHSELFDGKIEHVA
                     PTTEFTPRFLFSPTERANLVVRVRVRIDDPEERLHAGVPAFATFQMQEP"
     gene            13031..13861
                     /locus_tag="VFG22_11290"
     CDS             13031..13861
                     /locus_tag="VFG22_11290"
                     /inference="COORDINATES: protein motif:HMM:NF024981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HET6416871.1"
                     /translation="MLDLSIALFIGGVLFVAGLEVTLAQALEPLKNRPVLVRALLANI
                     VLAPLVVYGMSVLFPLERPFMIGVLLYGFAAGAPYTPKLVSMAGADVPTSIGITILLT
                     LLTIVLMPLVLPFLVPGTKIGIWEIAKPLLLQMFLPLVIGFSIRQFSQSLAAKLLKPA
                     NLVVNLSLLVFIVLAIVLHHEDLGATVGTGAITSAIALTLITFGVGYVLGPGGEKSKV
                     TLGLITTARNIGAAATIATANFGDEPKVLFTIAICMFVVFLIAFPAAKLFFHDRLSLA
                     "
     gene            complement(13858..14487)
                     /locus_tag="VFG22_11295"
     CDS             complement(13858..14487)
                     /locus_tag="VFG22_11295"
                     /inference="COORDINATES: protein motif:HMM:NF022766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3124 domain-containing protein"
                     /protein_id="HET6416872.1"
                     /translation="MSDDPNYPNWLLWFSEHGVRILILLAIAFMAFVYTRSRSNTEAR
                     LHAIEDSVQYEAPRSYTPPNLEAYAAKGISTRSLPVHQAAYVPVYSHIYYDGGRPYLL
                     EATLSIRNVDPKRPVYVSAVEYYDTNGKLSRKLIDRLIMLGPLQTIDFLIERHDVTGG
                     SGANFIVEWHASTHETHTPLIEAVMVGRSGTNAISFVRKSESLPQWMSD"
     gene            14603..14673
                     /locus_tag="VFG22_11300"
     tRNA            14603..14673
                     /locus_tag="VFG22_11300"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14635..14637,aa:Gln,seq:ttg)
BASE COUNT         2875 a         4546 c         4440 g         2943 t
ORIGIN      
        1 gcgtgcccgg ggcaatcgac gtgcgcgtag tgacggttgc ccgtctggta ctccacgtgg
       61 ctcgtcgcga tcgtcaccgt cttggtgtcg tcgcgctccg tcccgccctt cgcgatgtct
      121 ttgtagctct tgacctcgcc gccgtgcttg tcggacatca ccttcatgat cgccgccgtc
      181 agcgtcgtct tgccatggtc gatgtgacca atcgtgccga cgttgatgtg tggcttgttt
      241 cggacaaact tctctttagc catcgttagc tccctcagcc gcccttcgcc ttggcgacga
      301 tttcatcggc gatgccctgc ggcaccccct gatactcctt gaattgcatc gtgtagaccg
      361 cgcgcccttg cgtcttcgag cggaggtcgt tgacgtaacc aaacatgttc gcgagcggaa
      421 caaatgaacg aatcacttga ataccactca cggaatccat tcccgtgacg ttcccgcgac
      481 gcgagttgag gtcgccaatg acctcaccca tgtagtcctc tgggacacgg acctctacat
      541 ccatcaccgg ttcgagcagt tgaatgccgg ctcgcttcgc gccttcttgg aaggctagcg
      601 agcccgcaac ctcgaacgcg gcctggctcg aatcgacgtc gtggtacgag ccatcgaaga
      661 gcgtcgcttt gacgtcgatg acggggtacc cagccatcac gccgcggctc attgcatcct
      721 tgatgccctt ctgaacggcc ggaatgaatt ccttcggaat gacgccgccg atgatcttgt
      781 tctcgaactc gaaaccgcta ccggcctcca tcggctcgag cttcacccag acgtggccga
      841 acatgccgcg accgccgctc tgcttgatgt agcgaccttc ggactcgacc gatttggtga
      901 tcgtctcgcg gtacgcgacc tgcggggcgc caacgttcga atctacggcg aattcccgct
      961 tcaatcgatc gacgataatc tcgagatgga gctcgcccat gccgctgatg atggtctggc
     1021 ccgactcctc gtcggtatga gcacggaagc tcggatcttc cgcggcgagc ttcccaagcc
     1081 cgacgccgag cttgtcttgg tcggccttgg tacgcggctc gatggcgacc gaaatgactg
     1141 gttccgggaa gtccatccgt tcgaggatga tcggcgcctt gtcatcgcaa agcgtgtcgc
     1201 ccgtggtagc cgtcttcaga cccacggctg ccgcgatgtt gccagtgtga atctccttga
     1261 gctcttcgcg gttgttcgcg tgcatctgaa ggatgcggcc aacccgctct ttcttgccct
     1321 tggtcgagtt gagcactcca ctaccagcct cgagcatgcc ggagtaaacg cggaagaacg
     1381 tcagctgacc gacgaaggga tcgttgatga tcttgaatgc gagcgcgctg aacggctcgt
     1441 cgtccgatgc cttgcgctcc atcttcttct cgggatcgtc gggatcaacg ccctgaatcg
     1501 caggaacgtc cagaggcgaa ggaaggtaat tgacgaccgc atccaacagc tgctgcacgc
     1561 ctttgttctt gaaggcggag cccgtgagaa ccgggacgat ggtctgagcg agacaaccct
     1621 tgcgaattgc caggatgatc tcctcttccg agaagtcagt gtcgccctcg aggtagcgtt
     1681 ccatcaaggc gtcgtccacc tcggaagccg cttcgagcag tgcggcacgg gccttttcgg
     1741 cgtcagccct gaactcgtcg ggaatctcct cgacaatcca ctctgcgccg agcttgtctt
     1801 cggtgaagcg attcgccttc atcgctacga gatcgatcac accctgaagc tccgcctcct
     1861 gcccgagcgg aagctgaacg gcgactgcgg gcgcgcccaa gcgatccttg atcgactgaa
     1921 acgcggttgg aaagtcagca cccgccttgt ccatcttgtt gatgaagcag atgcgtggga
     1981 cccggtaccg atcggcctgt cgccaaacgg tttcggactg gggctccacg ccttccttgc
     2041 cgtcgaacac cgcaacggcg ccgtcgagca cccgaagcga gcgctcgacc tcgattgtga
     2101 agtctacgtg acccggcgtg tcgatgatgt tgatgcggaa ctgatcgcta ccaaaggggc
     2161 ctacgccggg tcgccagaaa cacgtggtgg cagccgatgt gattgtgatg ccccgctctt
     2221 gctcctgcgc catgtagtcc atggtggcag caccgtcgtg cacctcgccg atcttgtagt
     2281 tgacgccggt gtaatagagg atgcgctcgg tagtcgtcgt cttgcccgca tcgatgtggg
     2341 ccatgatgcc gatgtttcgc gtcttctcga gtgggaactt cctggccatt ggattctatg
     2401 agctcgactc ttttgactct ggcgctttac gtacaaaacc ccctccgacc cagaagccac
     2461 cctcgggctt cgtcaactca gagagggttt cgagaggcac atcggttgcg acgtttcgtc
     2521 gcggtcttcc cggtgactct ctctatgtgc gcgatgtcat gagttcagct gctgctcctt
     2581 gtccgagggg tttccgtaga ccttcccaga cgccaaaaag ttcctaccag cgataatggg
     2641 cgaatgcctt gttggcgtcg gccatcttgt gcgtgtcttc gcgcttcttc accgcgttgc
     2701 cacggccctg agacgcttcc atgagctctg cggcgagtcg ctcttccatg gacttctcgc
     2761 ctcgtgcccg tgagtactga acgagccagc gcatagcgag cgcgttgcgg cggtccgagc
     2821 gaacctcgac gggaacctga tacgtcgcgc cacctactcg gcgactcttc acctcgacgc
     2881 gtggcttcac ggcatcgagt gccttcttga acacgtcgag gggctcatcc ttgtagcgct
     2941 cttcaatgat attgaaggcg ccataaaggc atttctcagc cgtcgaccgc ttgcctgcaa
     3001 gcatcagcga gttgacgaac ttcgccacga gctgatcgcc gtacttggcg tcggggatga
     3061 tgtgtctctt gggtgcgctg actcgacgac tcatgacttc gacttgctca gttcttcggg
     3121 cgcttgacgc cgtactttga ccgcttgttg acgcggttcg ctttgttggt gttggaaggg
     3181 ccggctgcgc cggtggcgtc gagcgtgccg cgaacgacgt gataacgcac gccgggaaga
     3241 tctttcacac ggccgccgcg aatgagcacc acggagtgct cttggaggtt gtgcccctca
     3301 cccggaatgt acgtggtggc ttcgatgccg ttcgagagcc gtacgcgagc gaccttgcgg
     3361 agcgcagagt tcggcttttt cggtgtcgtt gtgtacacgc gaacgcacac accccgcttc
     3421 tgcgggcacc gctgaagcgc cggcgagtcc gttttggatt tgatctgttt tcgtcccttg
     3481 cggacgagct ggttgatcgt tggcataact atccgaaaaa gcgtacaaat cctctaacgc
     3541 gcgtcatttc cctacgcgtc aaagggacgg aaggataggc gtgaaagtcc tgctgtcaag
     3601 cgaagtgttc agggatttgg agataagccc caagtatctg aaataactaa catttctgcg
     3661 cctcccgcga gcctggtttt cgactgaggt tagcaggaat gatcccgaga catcgatcgc
     3721 ccgggggggc cccgaaactc agaaatccaa gcgaaaattc ccaccagtga ggcccagatt
     3781 caggcgaatt tgtgggccct tgctctcagc tgactcggca tccgggcgca ggtccctctt
     3841 cctcgtgctt cgaatgacgg gcttgaatcg gacttccgct tcgatgacgc cggccaaccc
     3901 aaaccccacc agggccccga cgcttaccca attgccgatg cgaagtgcgc gctcgactcg
     3961 ctgagcgcgt tcgatttcac tcggcgccgg gttctcgttt cgcagggagt tgtggccgat
     4021 gaaggttgcg atcgcggcga cggtgaaggc gctttcggtg acggcaaaca tgattccaag
     4081 cttgcgactc tcgttctgga actgtcctat gccgtacgga agcgcggcga tccagcgtga
     4141 attcaccttc tccaccgtct cggtggaggc gagctcctcg agcgccgcaa tgcgttcttg
     4201 ctggcggatc agtgcctcga gctcttccgt gcgctcttgc tgcttgcgtg ccgcactgag
     4261 agcctcctgg cgagcgcgtt ccgcagcgac ttccgtttga acttcctgga atgtctggac
     4321 gacggcctct ggaaaggcga ccgggtcgat gtcgtaggcg ggatcctcct cgagaagcgc
     4381 cctgaactgc tcgcgcgccg cttcgtcttt gccgaggaag aggtaggtcg cgcccaagta
     4441 tttccgggct tcgagccgga cgactgaatt gctggcccga ggaaccgccc caccgacgag
     4501 ggactcgagt cctctggcag ccttgctgta gttctgcttg tcgtagttgc ggcgagcgga
     4561 ttcaaaatcc gaaagctcgt ccgctgcgac tggaagcgag tacaccgcca tcgacaggac
     4621 tagcaaaccg gaaagccagg cgcgccgcat caccttaggc gggttctacg gggtttcgcc
     4681 ttccctctca agcgtgaagg cgtgctcgac caagtccccg cctgcgcgaa gctgaacgac
     4741 actcttgtac gttcgatacc cgggcgcgcg aatcttgacc tcggcagaag tctgcagcga
     4801 acgcatcggt attcggaaga tctcacgaat tcgaccgctg accttgcgac ccccgggaag
     4861 cgtaatttgc accgtggcat cgacctgcgt gggccctttg aggtacaggg tagccggctt
     4921 gaagcgaagc ttcacgggca gctcgaagtc tcgggtgccc gggggaatgc gctttcgcac
     4981 cacgcgctgc tcgcaacagt cctctgcgcc gacgaatcga aatgtgtgac tcccaacctt
     5041 taggcgaaca ccttggtagt cgggaccata ggccctgagt gggctcccat cgacactgat
     5101 cgatacgttt ggcggtgtcg gcttgaacac gacccagcga ggcgcattgg acgctgcact
     5161 ctgctcaccg tcgcgttcac tcgacccggg gtcgagatct tcggattcgg cattgcgccc
     5221 gctcgaggtc ttggacccat ctaccgggtc agccggcggg ggcggcgccg gttcgttggc
     5281 tttgccgccg aaatcgccct gcgattcgct gccggggtcc gcagccacgg ggagcgactt
     5341 gtccggacgg acggcccctt gctcaagcag ccaggccacg taggcagcgc cgccaaggct
     5401 cagaagcagt atcgcagcga cgagaaagat cagtgcgttt cgaagacggc ggcgtcccag
     5461 cgagccgagg agcttcagtg ccctctcgtt tccatcatcg atggcgagcg ctcgactgag
     5521 ggcggcctgt gcgttcagga catcacctgc cttcgccgac gaagcgccaa tcgcgagaag
     5581 cctttgtagc gagttctctc gaatctcggt gccgacatcg tccggcgagg ccaagtagcg
     5641 ggcgagcgtc tcgtccggct gctcgacacc catggccgcg agaaatgcgc gaaggtcttc
     5701 ggccattgcc gaagcacttt gataccgatc tgcaggtctg cgcgacaacg ccttgtcgat
     5761 gatggcagcg agatcggcgc cgatctcagg tcgcacccgg agggcatcag ggtactcgcc
     5821 ctcgagcagc aacttcaaga gatggtgggt gttgcgaccg atgaagggaa gccgcccggt
     5881 cgcgcagaag tagaggaccg tcccgagcga aaacacgtcg ctgcgcacat cgcaagtgcc
     5941 cccttcgacc tgctccggcg ccatgtgacc aggtgagccg aggatctgtc ccgtcgccgt
     6001 gaacgtgtgt gtatcgacca tgtgcgcaat gccaaagtcc gtgagtttca cgcaacggtc
     6061 ctcgtgaatc atcacgttct cgggtttcac gtcgcgatga atgattccct tcgcatgggc
     6121 ctcgctcagc gcttcggcga gttgaagtgc gatggcggca gcgatctcgg gaggcaggtc
     6181 cttctgccgc agcacgaatt ccttcaccgt gggacccgtc agcaactccg ccgcgatgta
     6241 cgtctcgtca gaggattcac cgctgtagtc gtagatctcg agaatgtggg gatgcctgag
     6301 cttggcgacg ctctttgcct cccgcgtgaa tctggcacga gcttcgctgg ttcgctgcag
     6361 gtgcggatgc aggaccttca gagcgacgaa gcggtcgagc gagctatcac gcgcccgata
     6421 aacagtggcc attccgccgt ggccaatctc atcgaggact tcgtacttct cgagcttacg
     6481 caacatcttc cccggaagtt cgagggtatc agcttcgggc gcctctaccc caaccttcta
     6541 taacggttcc agcgccgcgg gaacgcagaa ctcgaaatcg tcccggacgc ttacaccgtc
     6601 gtcgccaccc gcgacgatga acgccccgct ctccaagacg atccccccgg caccggcacg
     6661 tggaacattc aagtccagca gcggcgagat ttcgacgtct gtgccgctcc aacgaacctc
     6721 atcaacccgc gacgagccct ctccgccaat gacgagctgg cttcgctgcg gctgaagggc
     6781 tgccaaacgt gccgtgggcc aagcgggccc cgcgctcgca gtgcaaccag aagggcaatc
     6841 atcgaagcgc agagtgtcct gccgcaaggc ggcgtccgca tcgacgccgc ccatccagag
     6901 ggcgcgattc tctccgtcgc cgacgagcaa gccagctttg cgaaccccgt ccatgacccc
     6961 ggcgaccgat tgcccaacct gttccccttc gagaaggatc tccgctgtcc cgtcgtcgtc
     7021 gccccctgcg accagaacat ccattccaag cgccgtcgct tgcggatcgg atcgcgggac
     7081 gctcagtcga agggagacca cggttccatc gggctcgacc agggacacgg tcgactgggg
     7141 cacgcccgat acctccccac cgattaccat cgcaccgagt ccgggcacgg atacgagagc
     7201 cgagcgcgta ccggcaccgt tgtgcaggat gacggccgtt attctccgcg tagggtcgaa
     7261 catgtcgaag atgaaaggcg aggcgttgct cggaagaacc aagatcttgc cttcatcgat
     7321 cgaagcggcc cgcgtctctc cgagctcgga caaagagaat tcttcctcga acagatgcga
     7381 cttccattcg agcacgtcga ggtactcgac ctgggcatcg gtcgagctag ccccacccac
     7441 caggagcgcg aaagtaccgg actgcaccat gccgaagaaa gcgcgaggcg cttccatgct
     7501 gttgagcaag acgcgctcgc acgatgacgg cgcagcggtc acgacccgca tgaaccccgc
     7561 gctcgcaccg ggaacaaacg cgggcggcgc agtcaagagc tcggtagtcg cgccctcgat
     7621 ttcaacccgt acccgcgttg gcgccgcgaa gacacggaac tctagcaccc cttcgaaatc
     7681 gccttcggtg attgggtact cgtactctac cggcgcgagc tcgccttcct gtatgccaac
     7741 tcgaagggtt gtcgcgtcag tcccggccgc cgggtttcca ccctcgccgt cgacgatgac
     7801 cgcctcgaac acgaactgct cgacactatc gttgcaccca aagactgaga ttgagagcaa
     7861 tccaagcaac ggcaatcggc caaacacgcg ctgaggttgc tttaaggctc gacggcgggc
     7921 aagtagagca gcggagtgtg ggccgaagac gtcgcttcga tagaagagct ggctgtcgcg
     7981 cgacgactga gcgcagagcc cggcttcgct tggctggatt cgaatcgaag cgaggcgcga
     8041 gacgggcgct attcgttcat tgccgcgtgg ccatgcgacg agatccgggt cgagttcggg
     8101 gctgaagctc ctttttccgc cctccgcggt ttgagccggc ccgaggcggt ggtgaacgat
     8161 caggggccga agccttgcga agtaccggcg tggattggct acatcgcata tgacgcagct
     8221 tggtgcgcgc ctccacgagg agagcctcgc tttgaaagag agcgcgagcc agtgcttttg
     8281 tttcgacgat actctgccct catcgcgatc gatcacgagc agaacaaagc ttggatcgtc
     8341 ggcgatgacc aaaatgcctg cgacgagttt ctcggacggc tgcgaggcct cgtcgatcgt
     8401 gagggttcgc tcaccaagat cggtccagtt gaagtcgacg atgccgcgtc gcaccagaat
     8461 gcgatcgagc gcgcgctcga ctacatctcg atgggcgaag tctatgaagt aaacgtcgcg
     8521 agacaatggt cggcggcttt tgagggcgac gcgctcgcgc tctggacggc gatgcgaatt
     8581 gccagccgag tgccgctcgg aatgtacctc gacgccggtg accattcggt gctggcctgc
     8641 acgatggagc gcttcatcca ctgggacgcc gagagcaaga ccctcgtgac ccgtccgatc
     8701 aaggggacga ttcggcgcga agcaaccgat gacggagcct ccgaacgact gcgcgccgat
     8761 ccgaaggagc aagccgagca ttcgatgatc gtggatctca tgcgcaacga cctgggccgc
     8821 gtcgcagaga cgggaacggt gaaggtcgac gctccgctga tcgtggaacc gttcacaggc
     8881 ctctatcatc tcgtgagcac ggtcagttgc gtgacgagac ccgatgtctc gctcgaaagc
     8941 attctggaag cgactttccc gccagggagc gtcaccggtg caccgaaagt ccgcgcggtc
     9001 gaaatcatcg aagagctcga agaacaccct cgcggcatct attgcggggc gattggcttc
     9061 atcgaccacg acggtgggat ctcgctcgcc gttgctattc gaacggctac ggtcgcaaaa
     9121 ggaaaggtca cgtactgggc gggcggcgga atcgtcgaag cgagcgaccc gggtcgcgag
     9181 gttgaggaaa ccgagctcaa ggcacgggtt tttctcgatg ctatagaatc acttcggaga
     9241 gcgagcggag atgagtgaac gtattcaaga catcctggat ttctggttcg gcgagctcga
     9301 cgagcacggc tgtgcgagcc ccaaccagcg gaagcgatgg tgggcgaagt ccgacgcatt
     9361 cgacgagacc gtcaaggccc agttcctcga cgactacgag gccgtcgtag atggggagcg
     9421 agaggcgtgg cgaaacacgg cgcatggagc tctggcttac atcattgtct tggaccagtt
     9481 ctcgcgaaac atgtttcgtg gcacgtcgaa gatgttcgaa gccgacgagc tcgccagaga
     9541 cgtctgttac gaaggtctag attcggggtt tgacatggag ctaggattcg acgagcgcgt
     9601 cttcttctac ttaccgctcg agcacagtga gtcgatggat gaccagatgc ggtgcctcga
     9661 tatgttcgag accctggccg aagacgcacc cgagcatctc caagtcgatg cgaggaacta
     9721 tctcgacttc gcgaaccgtc atcgagcgat catcgagcga ttcggacgat acccacaccg
     9781 aaacgacgcc ctgggacgag cctctactcc cgaggagcgg gagttcctcg agacccccgg
     9841 ctcatcgttc tagtcgagct tcttcttgaa gcgcgcggag acgacgatgg tcagaaagag
     9901 ggttaatccg gcgagccaaa cgagctcgga gcggagctcc atgaaggagg ctcccttgag
     9961 cacgatcccg cgaatcactc gaaggtagtg cgtcagagga agcatcatgg acaaccaacg
    10021 ggcgggtgcc ggcatcgctt cccagggaaa catgaaacca gacagcagga tgtttggaag
    10081 cagaaagaag aacgacatct gcatcgcttg ctgctgagtt ttcgcgagcg tggaaaagaa
    10141 gagcccaacg gcgagactcg cagcgatgaa gaacacggag agcccataga gaagggcgac
    10201 ggaccccacg attggcactg cgaaaaccca agaccccacg accaaaatca gtgacatctg
    10261 caagtatccc acggcaatgt atggaaggat cttgccgatg gtcagctcac cgctgcgaat
    10321 cggagacacg atcagctgct cgagcgttcc gcgctcgcgc tcccgagcga tcgccatggc
    10381 ggtgaacatc accaaggtca tcgtgaggat tacacccacc aaaccgggca cgatgaacac
    10441 cgcggtgcga agatcgggat tgtaccaagt cgtcggctct agggcgatta cttgcgatga
    10501 tatcgatgcg ggctgtccca aacgttgcag tcgctcgacg gtgagagcgg aagaacgtgc
    10561 cgcggcaagc gacattgcag tattggtcgc gctcgcgacg acctgtggat cggagccgtc
    10621 gaccacgagt tgtagctgag ccatccttcc ggcttcgacc tccgccccga atcgcggggg
    10681 aacgaccagc gctaccatgg cctggcgaga acgcagcgcc acgccgattt gctcgtagct
    10741 ttccacgtaa ccgacgactt cgtagaagcg tgtcgcctcc atgcgccgga cgaagccgcg
    10801 ggactcctcg gaatgatcct gatcgtagac cacggtcgcg atgttgcgga catcggtgtt
    10861 gatcgcatag ccgaacaaca gcagctggat gatcggaatg ccgatcatca tccccatggt
    10921 gagacgatct cgacgtgctt ggataatctc cttccacgcg atcgcaaaca ttcggttcat
    10981 acgctgcttc cgctccgtgc ctcgctgtgc accatcgcga cgaacgcgtc ttcgacattg
    11041 gggacgatct ctcgggcatc gcgaacgccg ggacctcctc cgaggacttc gtgactgagc
    11101 gccaccggat ctacaccacc gagcgtggcc actctgagac tgtccccaaa cggcgcgacc
    11161 tcgtctacgc tcgggtgcga agtcaggaga tccattgccg cggctgcgtc cgaagtcgag
    11221 atgatggcga tgcgaatttc gcgacgctcg atgacctctg aaggcgtgcc gagatcgagc
    11281 agagtgcctc tgaaaatgaa tgctagccga tgacagcgct ctgcctcgtc catgtagtga
    11341 gtcgtcacca tcaccgtggt tccgccggcg gcgatgcgat ggatgtgggc ccagaactct
    11401 cgacggctca ccgggtccac gcccgcggtg ggctcgtcga gaaacagcaa tggaggctgg
    11461 tggattgtcg cgcagacgag agcgagccgc tgtttccagc ccccggacag agttccggcg
    11521 atctgatggc gacggtcgtc gagccctgag cgaacgagct cctcgggaac gcggacgcgg
    11581 cgctcggcgc gcccaaggcc gtagataccc gcatagaaac gcaggttctc ctcgaccgtg
    11641 agatcttcgt agaggctgaa gcgctgagtc atgtagccga tgttctgctt gactcgttcc
    11701 ggctggctcg caacgtcgaa ccccacgaca gtggctcggc cggcgcttgg atcgaggatc
    11761 ccgcagagca tccggagcgt cgtcgacttg cccgcgccgt tgggcccgag cagtccgaaa
    11821 atctcaccac ggtggactcg gaaactcaca tcgctcaccg ctaggagctt tccgaaatga
    11881 cgggagagcc cctcgacgac cacgggatcg tccacggtta tggttcctgc atctgaaagg
    11941 tggcgaacgc gggcacaccc gcatggagtc gctcttctgg atcatcgata cgaactcgaa
    12001 cacgaacgac gagattggcg cgctcggtgg gcgaaaagag aaaccgcggt gtgaactcgg
    12061 tcgtcggtgc aacgtgctcg atcttcccat cgaacaactc ggagtgctca tccactcgaa
    12121 cgttcgcggg caagccgacg cgcaagcctg tcagtgcttg ctgtggcacg aagacgtcga
    12181 cgtaaggatg cgtcgtatcg gcaaccgtag cgaccgttgc accagcggca accacctcac
    12241 caactctcac tgggacgtca agcacggtca cgcgctcggc agcctccatg gagtagagag
    12301 ccagtctctc cctctctgct tcgacagccg cctcggccgc ggttacctcg gcatcgacag
    12361 ccgcgagctc ttcaggccgt gctcctatgc ggagctgttt gaggcgctcc tgaagtacac
    12421 gaacttgagc tttggactgc gcgtacgcgg cttcgagctc cgagactagc gaacgagcgg
    12481 tcgccgcctc tttgggtaga tttcggtggc gatcgaggtt ggctcgggct tcatgaagcg
    12541 ccgcgcgtgc accgtctagc tctgccatcg tggacctgac ttcctcgacc cgtggcccgg
    12601 cgtacacgag ggccgcattg gctcgagccc gggcgagctc agcttcacga gccaggagaa
    12661 tcggtagctc agccgtgtcg tcgaggatcg cgatcacggc gccttcttcg atgatgtcac
    12721 ctcgatcgaa gcgaatctcg cgaagctgtc ctccgacacg gaaaccgagg tggcgatcgt
    12781 cgtgctcgac gatcccctga tagcccgctg gcatcgcccg ctcccggtcg catccgcaaa
    12841 gggtcacgat gatgatggcg cccatcagca ggccagcacg gaccaatgtc gtcatgaccg
    12901 ggtcaacggt agcagcctca ggccgttcgg ggaagcaagg gcacctgcta gatcgtcaag
    12961 gtcgttgcca tcggtagcaa cgctggtgta acaaagcgcc gacgcatcga tgcatgaggc
    13021 cttgaagata gtgctggatc tctcgattgc gctcttcatc ggcggagtgc ttttcgtggc
    13081 tgggctcgag gtcacgttgg cgcaggcatt ggagccgctc aagaatcgtc cggtcctggt
    13141 aagggcactg ctcgcgaaca tcgttctagc gccgctcgtc gtgtatggca tgagcgtcct
    13201 gtttccgctg gagcgcccgt tcatgatcgg tgtcttgctc tacggttttg cagccggagc
    13261 accgtacacg ccgaagttgg tgagcatggc cggcgccgac gttccgacct cgatcggcat
    13321 caccatcctc ctcaccctac tcacgatcgt cttgatgccg ctcgtccttc cgttcttggt
    13381 ccctgggacg aagattggga tctgggagat cgcaaagcca ctcctcttgc agatgttctt
    13441 gcccttggtg attggattct cgatccgaca attcagccag tcgttggcgg ccaagctact
    13501 caagccggcc aatctcgtgg tcaacctctc gctcctggta ttcatcgtgc tcgccatcgt
    13561 tctacaccac gaggacctgg gcgcgaccgt aggcaccggc gcgatcacct cggcaatcgc
    13621 actcacactg atcacgtttg gcgtcggtta tgtgctcggc cccggcggcg agaagagcaa
    13681 ggtgacactg ggcttgatca cgaccgctcg aaacatcggc gcagccgcga cgatcgccac
    13741 cgcgaacttc ggtgatgagc ccaaggtctt gttcacgatt gccatttgca tgttcgtggt
    13801 gttcctaatc gcgtttccgg cagcaaagct cttcttccac gatcggctga gcctcgccta
    13861 atccgacatc cactgcggca gggattcact ctttcgaacg aacgaaatgg cgttggtgcc
    13921 cgagcggcca accatcacgg cttcgatgag cggagtgtgc gtctcatggg tcgacgcgtg
    13981 ccattcgacg atgaagttcg ctcccgatcc gccggtcacg tcgtgccgct caattagaaa
    14041 atcgatcgtc tggagcggtc cgagcatgat caagcggtcg atcagcttcc tcgagagctt
    14101 gccattggta tcgtagtact ccaccgcgct cacataaacg ggacgcttcg gatccacgtt
    14161 ccggatgctc agcgtcgcct cgaggaggta gggacggcca ccgtcgtagt aaatgtggga
    14221 gtagaccgga acataggcgg cctggtggac cggcaacgaa cgcgtactga tccccttcgc
    14281 tgcgtaggct tccaaattgg gcggtgtata ggagcgcgga gcctcgtact gcacgctatc
    14341 ttcgatcgca tggagccgcg cctcggtgtt gctgcggctg cgcgtataga cgaaggccat
    14401 gaacgcgatc gccagcagga tgagaatacg aactccgtgc tcggagaacc agagcaacca
    14461 gttcggataa ttcggatcgt cgctcaccga aacacacccc agcggttcat ggccccgggg
    14521 tatcgcacta atgccgggcc tcccgcaagc cccagctgcc gacgcttgag gagccgctca
    14581 tgcgtgttat taggagggac gctcccgggt cgtctaaggg caggacaaca ggctttggac
    14641 ctgtgaatgg tggttcgaat ccacccccgg gaaccgagtt caaatatgca ggccaatacg
    14701 accgctttca tgatcctcgt cgcggcactt gccatgagct gtaagaaggc agacgactcg
    14761 aacggcacag cgaccttgcc atcgttgggc ttgggctgcg atca
//
LOCUS       DASRZF010000255        20276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4998537, whole genome shotgun sequence.
ACCESSION   DASRZF010000255 DASRZF010000000
VERSION     DASRZF010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 20276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20276
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4998537"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..2373)
                     /locus_tag="VFG22_11305"
     CDS             complement(196..2373)
                     /locus_tag="VFG22_11305"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET6416873.1"
                     /translation="MLDLSSLNSPQREAVVHEGGPLVVFAGAGSGKTRVITFRVAHLI
                     SEHGVAPWRILAVTFTNKAAGEMRQRLLDLLGEQGSAVQVGTFHATCARLLRRHGESF
                     GLSKDFTIYDDQDQLALVRRVLRDLGLDEKRYQPKAMAGRINRAKQEVQGPEDIETPD
                     AWSEIVRRVYTAYEERLRKANALDFGDLIYRTVRGIESEESFRNAVVGRFSHVLVDEF
                     QDTNHAQFRLVRLLSEGHQNLCVVGDDDQSIYRWRGADRRNILDFRASFPDARLVKLE
                     QNYRSTRRILRAANAVIARNTDREPKELWTDNQEGDAILVVRTMDERDEARLVLRGVR
                     ELISQGHRLSDVAVFYRTHAQSRVIEESLRSANIGYRIVGGMRFYDRAEVKDLLAYLR
                     VMTNPADDMSLLRIINTPTRGIGKTTITRLLDSAAAYGQSLMDLLGDDATLDQFGSAA
                     RKRLNAFHGLMSGLREVGASGAPLGEVGAELVNQCGYGEMLRHDDTPEADARYQNVQE
                     LVASMDEFQRERPDGTLSEFLENVTLQTSADEQEGGDRVTLMTVHAAKGLEFPVVMVT
                     GLEEQVFPFKGIDPWEDPEELEEERRLAYVAFTRAEQRLVLSFAAVRRIFGQQRVGIP
                     SRFLTELPNEDIEWIGHRSTSPGLGPSTESRGREPSESYVDYSEGSDLMEVEGLRRGT
                     EVRHPKFGVGEVQEVMQGSPPRVSVRFPGWGTRKIIASYLEPA"
     gene            2532..3191
                     /locus_tag="VFG22_11310"
     CDS             2532..3191
                     /locus_tag="VFG22_11310"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6416874.1"
                     /translation="MNQPLSSASKPEESSDESSKKGDHDLVRRVQSGDSAAFRVLFDK
                     YHRRAFAVAMGVVKNQDDALDAVQEAFVKVHKNIHKFEGSSSFYTWLYRIVMNASIDH
                     VRRTSRRKSLDFDERHLHEESDVAGDGALVPNITDANPGKAALRRELGSAIETALQGL
                     PEHHRAVIVLREIEGMSYEEMAQVLEVPKGTVMSRLFHARKKMQDSLAPYLSGEAVSE
                     D"
     gene            3194..3739
                     /locus_tag="VFG22_11315"
     CDS             3194..3739
                     /locus_tag="VFG22_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416875.1"
                     /translation="MESPKPEDQKLQMFFDGELPPEEEAALRRSLESTPEGMAQIREW
                     AQIREAMQTASADWSGQIDSDSLFARIEAEVSAPVIPIEPARQAKPPLHSVPGGRERR
                     VWGAVATGFAAAAAIFLTVMAWPETHQDPSGVTAEVRTRGSEVLEVDFGTNTGTIFEV
                     EGGAGESLAVVWIDDGEVGLQ"
     gene            3736..4236
                     /locus_tag="VFG22_11320"
     CDS             3736..4236
                     /locus_tag="VFG22_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416876.1"
                     /translation="MSLIRYLAMIAVLLGASNASAQGSSSVRSDRDSTSSVPAEVLVV
                     LAKEEPGEIDPELKKLTALRRPPFNSFRSMQILSRPKLTLTPGKDALVSLPNGRRVKL
                     TLLRVMPDGRYKVKAAINRPDKSDYLPLLQVVASAGDPFFVAGQTYQGGTLVVGVIVD
                     PKKTSQ"
     gene            4237..4929
                     /locus_tag="VFG22_11325"
     CDS             4237..4929
                     /locus_tag="VFG22_11325"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET6416877.1"
                     /translation="MRADDPDAIEVEGPPRLEHPAEVVIEALSKVVTSKRLERIQDVA
                     RSRTDNLVLVLDRIADPHNSSAVLRSADAFGVQRVHVIVGEQGFRASRGVSKGTHRWL
                     DVVRYETADACARRLRDENYAIFVAAMGGETRLEDLRVAPRLAVVFGNEHRGVSPEMQ
                     DLADGTFSIPMCGFVESLNISVAAAITMQTLAGDGRARLPDARCRELEARFLMNSVRN
                     AGQLIEKFIGND"
     gene            4999..7425
                     /locus_tag="VFG22_11330"
     CDS             4999..7425
                     /locus_tag="VFG22_11330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HET6416878.1"
                     /translation="MAGKRTQKKKRKPSKRKKRSTGGSSALLKLVGAMLIVGALLLMG
                     AYAYFSRDLPAVSSLRDFKPPQTTRVYDRNGKVIGEMFAERRTVIPMSQVPRNVVLSV
                     LAAEDADFYEHEGLDYRGILRAVGRDIVAGRAAQGGSTITQQVVKLMLLTPERTLSRK
                     VRELILARRLENELSKDEILHLYLNLVNFGHGRYGIQEAARYYFAKNASDLSLAESAM
                     LAGIPQAPARLSPRSHPEAAKRRQEYVLSQLLAKRETYWPDLSEATIAAAIKEKVEPT
                     ALPEGAGNAPEVLSIARAALNAAIGEDAMGEGGYKVYTTIDLSLQMRAREALQRGLRA
                     IDERQGYRGPFRRTQRKSGPKPSKAQETLRMGRTYVARVMDADDENEKIWLDVGGHSA
                     VLRMRDAARYNPDGRKPTGFAKKGDLLPVSITQMGDDDDQDPAVVRLERGPEGAVVVI
                     EPRSRAVLALVGGYAALSGFNRATQAIRQPGSTFKPIVYARAIQSRRFTAASMVVDAP
                     AVYDEWKPRNYEQWNYQGAVRLREALARSINMVAIRVVEDIGVHDVAEFARQDGITTK
                     LEEDMALALGASGVRPVELANAYATFAAGGRWAPTRVVTRIVAPDGTRVPLEPDEPAR
                     DVMSPDEAYVITSMLRSVVTEGTGVPAQRLERAVVGKTGTSNEAHDAWFVGYSPSVVA
                     GVWVGFDDQRSIGKRESGTRTALPIWIDVMEAADQTPKDTDFAMPSGVIVARIDPASG
                     LLAYEGQEDAFEEVFLTGTAPREVARPPDVADPNRFLMEQYDEEDAGAAANRPRPSRE
                     ERVLGGVAPP"
     gene            7422..9017
                     /locus_tag="VFG22_11335"
     CDS             7422..9017
                     /locus_tag="VFG22_11335"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6416879.1"
                     /translation="MNRFGTRVVILFALAATFPVALTALVWLGVSQWGAAGESGEAAL
                     ARMQLNQLDERRKIAIDRFCRDDLVIDTLLDDRARETPPDIDYERLLRGAMQAAGLQV
                     LWVLDSLSGEVIASGHRNGTLGRDGAELMRQARTAHDRAFVFSLGDQEHQSFVLRSCS
                     IARGGAEVTVVGGHRFSELRSLDPRQLVIGREALKGDEIVAELGDAEGVVQNLVVWRL
                     GVDRRGPPLLLWAVSVAVLALGLALLFGGYLNRWLRSSVDELTEAATRVGRGDFDTTL
                     RDGFGGAFPATATAFNRMTRDLRDAREQLRQTERIAAWQDIAKSLAHELKNPLSPIRL
                     SIETLRKAHERSHEDFDAMFDESTRIILQEVDRLRAIVDEFSRFARLPAPQRRATDLG
                     EVLAQAAALHAEGEAPVRFELPDAPVVASVDSDQITQVLHNLLQNARDAACSAHPHGG
                     ALVRMSLEGRADTAYIRVEDNGPGIPTDQIEAIFEPYYTRKHGGTGLGLAITQRIITE
                     HGGRIDVESQAGRTVFTVVLPRR"
     gene            complement(9021..10211)
                     /locus_tag="VFG22_11340"
     CDS             complement(9021..10211)
                     /locus_tag="VFG22_11340"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HET6416880.1"
                     /translation="MTVLFSYAFRPLFMLATLYAIIVVPLWAAAWLGHLPMPNTLGTP
                     SWWHAHEMIYGFSGAAIGGFALTAVATWTKRPPVSGVLLIVLSALWVLARLLFASSES
                     TMLVPACAADLGYGALLFALMSREVITARSQRNYKVLLILGLLPVSNAVFFLGVARNA
                     SWARNALLAGLWLVVLLVNLIGGRIIPGFTRNWINRRLGDRKHAPVRLPPPFERIDLV
                     ASWLMLGFAISFVAGAPSRWLAILGLVTSLVMFVRLTRWQGIYTGDEPLVWVLHVGFA
                     WLPLGVLMLAAASLDLLPASAGIHALTSGAITTMIVAVASRAALGHTNRSLESHPILT
                     ASFVLITLAAIFRVAATFGPGARVLLALSAIAWCLGFACFGWRYLPILTRPKVQRPGS
                     LPTV"
     gene            complement(10240..11565)
                     /locus_tag="VFG22_11345"
     CDS             complement(10240..11565)
                     /locus_tag="VFG22_11345"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6416881.1"
                     /translation="MLIIDDDAAFRFAMAKALRRSGHEVSEAASGEEAVEVLSNSAAP
                     DIALLDLKMKGIGGLEVLRRCGPIPTRVIVLTGHGTVKTAVEAMQLGAFSFLEKPVDA
                     NMLRPLLQQALVEKGRTESGSTPETTPLVGTSPAMEEVRAFIRTVAPTNETVLVFGET
                     GTGKEVVARQIHAQSQRAARPFVAMNAACVPRELFESELFGHKRGAFTGAQNDRAGLF
                     READGGTLFIDELAELPLESQAKLLRAIETKSIRPVGDSGETEVDVRIVAATNRDLWT
                     ETQAGRFREDLYFRLQVFPVLIRPLRERLADIEPLTLHLLARLGKSGIQLGAESLEAL
                     REYDWPGNVRELLNVLRRASLFAISDTMDGELLRRMIAASVFGNARESVLPSSSDQSG
                     RVSLADVERDHIERVLREKDGNITKAAAALGIDRRTLQRKLRAYGMETQ"
     gene            complement(11580..13304)
                     /locus_tag="VFG22_11350"
     CDS             complement(11580..13304)
                     /locus_tag="VFG22_11350"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6416882.1"
                     /translation="MASEPTRSSVFFDSLERDDFTADRIRWLINLRWVAMAGVLFATG
                     LVLAGYFPGVAWPVLLGVVVVGSLYNFLLTRRAPAPGKRAAVSQALVDLVMLTVVLWA
                     AGGISSPFIGFYVFHLALIGILGGPGATLVATAAAFAGAGFLALSEWVPALKIGSWDP
                     APPWDTIGDVAAFMTTVGAAAYVVLHAVRELRDRERALARARDQAELEYEVISNTLDE
                     LEAGLEVVSPDGRIEWRNRLADELAPLPNAEGMWECPIAKRECTTQPAGLCPVWRARD
                     DRAEGRCGFSVGDRSYEMLSFPLGSRDDEPARIMNLYVDRTQILHAERQLLHAERLAS
                     LGRVAQGVAHELNTPLATIRTLAADMREAIRSLGGDEALQSDLAESAALIRDETGRLG
                     RITHSLLTGGTLPRLRLNTSAPLLPVVERARALVFAGVREGGPELHIGDSIENLDVVA
                     DPDRLLQVLVNLLQNALDSNREAGGMSVWVHAERRGSSVEIIVDDQGVGIHPDIAERL
                     FEPFATTKPPGEGTGLGLYASYMLVQTMGGDLTIENRAEGGASARVRLSLGAETLANE
                     PGVHHRAP"
     gene            13397..13918
                     /locus_tag="VFG22_11355"
     CDS             13397..13918
                     /locus_tag="VFG22_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416883.1"
                     /translation="MVITILLTSTLLTMLGIAGVLRGAYDLSLAPLPEDVRELYTPDV
                     EVTEAEVQLLEAQVTNPYRRPMAAANVVVSALVLIGSFMLSWRRRLAQWWIKQAVVAK
                     LIWIVAYTVSLISHIKLSFVPLPIEQPGAALQEVMAGVVLVSTFSAALHLAAAYRATR
                     PDIREFIETTAKP"
     gene            complement(13922..14659)
                     /locus_tag="VFG22_11360"
     CDS             complement(13922..14659)
                     /locus_tag="VFG22_11360"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6416884.1"
                     /translation="MKAAILGATKGMGRSLARLMAERGDAICLLGRDSVDLERSAADL
                     SVRGAFGIVACVRCDLEEPGMFAGAIDAARESLGGLDAVIVTAGVFATQDELEADLEL
                     TERLLRIDFTNTVLFCEAAKQALLSEGGGTLCAFSSVAGERGRKPIILYGAAKAGLTH
                     YLEGLDHKHRANGLRVITAKPGFVKTSMTAGLEPPPFAGEPEAVAKRVLRAVDRGTPV
                     VYAPAIWGLVMAVIRALPRAIMRRINF"
     gene            complement(14661..15989)
                     /locus_tag="VFG22_11365"
     CDS             complement(14661..15989)
                     /locus_tag="VFG22_11365"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET6416885.1"
                     /translation="MREPPLSTLSGWGRHSAVGREHFGEDIERMSADAHLSRGLGRSY
                     GDASLPPDDRPDVLNTTFADRILGFDEETGLFRAESGLALSELNRLLMPRGYFTPVSP
                     GTKFVTLGGMVAADIHGKNQHKEGNFGHHVTALKMRVADGRVLECSPEQHPDLFRATI
                     GGMGLTGHILEVEFGMHRIPSRWLWQENRRIHDIDEFQDALEEVAPEWPYTMGWIDCL
                     AGGENMGRGILMTARWAEAEEAPLRPPKPGFQPSLRFDWPSWALSGLSIRTFNELYYR
                     KQWRPRSSGIVNYESSYYPLDAIRAWNRMYGRRGFTQYQCVLPREAGRSSARRVLEVL
                     TARGGASFLCVIKDFGRESRGILSFPRPGITLALDIAIRDDTQALVDALNEQVLQEGG
                     RIYLAKDSFTRREHFVAMEGSRLDEFLAVRDRWDPDRRFRSAQSVRLLGD"
     gene            complement(16025..17194)
                     /gene="metK"
                     /locus_tag="VFG22_11370"
     CDS             complement(16025..17194)
                     /gene="metK"
                     /locus_tag="VFG22_11370"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HET6416886.1"
                     /translation="MSKYLFTSESVTEGHPDKICDKISDSLLDAYLEQDPKSRVAVET
                     MVKTGFVVCGGEVTSKAVIEIPKIVRKAITEIGYTDSSMGFDAETCGVLAAVEQQSPD
                     IARGVNSSATKEQGAGDQGLMFGYAVDETAQLMPMPIDLAHKLARKLTQVRKKNVIEG
                     LRPDGKTQVTVEYEDDKPKRFDAIVVSTQHAASVKQRALREAIIEEVITPTLPKKMID
                     NKTKIHINPTGRFVVGGPYGDAGLTGRKIIVDTYGGMGRHGGGAFSGKDPSKVDRSAA
                     YFARYVAKNIVASKVASRCEVQVAYAIGVARPMGVYVTTFGTGKVPDEKIGKAVAKLF
                     DFRPAAIVETLDLLRPIYVPTAAYGHFGRTEKTFTWERTDRADELRNALMPKRGR"
     gene            complement(17321..18610)
                     /locus_tag="VFG22_11375"
     CDS             complement(17321..18610)
                     /locus_tag="VFG22_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416887.1"
                     /translation="MRLRFALIVALVALGGCDLTIPNGIFGCGQPSDCPSDYFCWSSD
                     SRCYDTKEPECVPKTCDEVISDFASLGISIECGALPDGCDGSVECGSCPAGTVCGANG
                     ENFACGCEENSCANYGGGAECGAVPTRCGGQAEAIFCGDCLGDGFECVQNECVCPPGV
                     DCGGGCGGSCRDGEVCVGGECCEPSYPCAQNECSPPGGLPNGCGGFTQCPACGNGEDC
                     VLSDNLRYQCLGACTCEAQGVECGSTSICGSPTLCGTCADNGFEPGYHCESGRCVCED
                     SFEYNDSFETFSLICGESLGINCMQAAWSVDVQATLHDSRDVDYYALRVLDARTPIIA
                     QAYNGKSKRVLSMTYLCPDGFDGLDKCSGSTEKIDGIEFCIVEGDWIGIERKCDSNVS
                     SQVGTVLVGVESREFEGDCDPYGLKVLATYGTEIPTL"
     gene            complement(18610..19380)
                     /locus_tag="VFG22_11380"
     CDS             complement(18610..19380)
                     /locus_tag="VFG22_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6416888.1"
                     /translation="MRVLLVLVVSLSCLVAAVGVRAQSTESRDARIAEAKKAFSIGTQ
                     AYADGEFDTALASFQRAYELTGSPDLLFNVATVSDRMRRDEEALEAYEAYLQARPDSA
                     DREHVEGRIEVLRAAIEERRRAEIEADIEARKAAVEAAAKVRAERPLTQHVGPGPGPW
                     ITIGMSGAAVLGGAVLLGMGQRNENQVTSAPPGSSYSTFQDVADRGPRLSKAGIALLS
                     VGGAGLVGGVIWQLTGGHEEALPEISIGPTGISVKGRF"
     gene            complement(19450..>20276)
                     /locus_tag="VFG22_11385"
     CDS             complement(19450..>20276)
                     /locus_tag="VFG22_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET6416889.1"
                     /translation="RSGEAFMERHHDMKDLAPRDVVARAIDYEMKLRGHDCVYLDMTH
                     EPADFILERFPNIRARCLEYGIDMTKEPIPVVPAAHYMCGGVVVDDEGCSTLPGLWAV
                     GEVTCTGLHGANRLASNSLLEGLVYGHRTAIAARKALSLLPEVADEYVADWDVGEAVP
                     SDEAVVVSQNWDEIRRFMWNYVGIVRTDRRLRRAARRISMLQEEIREYYWAHLVNRDM
                     LELRNIADVAELIIRCASARRESRGLHYNLDYPHAGEEWRGDTRISRNTAPYIAHE"
BASE COUNT         4040 a         6289 c         6257 g         3690 t
ORIGIN      
        1 tctgacctct actccggacc atcgaaccga agcgaaagca gctcaaagag caaggaatcg
       61 agctcggcct cagctcccgc agcgccagcg agccccaagc cgaccaagcc gaccaagtcc
      121 gacgcgggta gctaggccct atcgtcacgc agagaggccc tgccccgcgc cgactcgaac
      181 agaatcccag tctgcctatg cgggctccaa gtaggaagcg atgatcttgc gtgttcccca
      241 tcctgggaac cgcacggaaa cccgtggggg agatccctgc atcacctctt ggacctctcc
      301 cacgccgaac ttcgggtgcc gcacctctgt cccccgccgc agcccctcga cctccatcaa
      361 gtcggagccc tcgctgtagt ccacgtagct ctcgctcggt tcgcgccctc gcgactcggt
      421 cgaaggacct agacctggcg acgtcgaccg gtgaccgatc cactcgatgt cttcattggg
      481 aagctccgtg aggaaccgcg aaggaatgcc gacgcgctgc tgtccgaaga ttcgtcggac
      541 ggcggcaaag ctgagcacca agcgctgctc ggcgcgggtg aacgccacat acgcgagacg
      601 gcgctcctct tcgagctcct cgggatcttc ccagggatcg atgcctttga atggaaagac
      661 ctgctcctcg agccccgtga ccatcacaac cggaaactcc aagcccttgg ccgcgtgcac
      721 ggtcatcaag gtgacgcgat cgcccccctc ctgttcatcg gcagaagtct gcaaggtcac
      781 gttctcgagg aactcggaca gcgtgccatc gggccgctca cgctggaact cgtccatgga
      841 cgccacgagc tcctgaacgt tctggtaccg agcatcggcc tccggcgtgt cgtcgtgccg
      901 aagcatctcg ccgtatccgc attgattgac cagctcagcg ccgacttctc cgagcggcgc
      961 tccagacgct ccaacctctc gcaagccgga cattagaccg tggaacgcat tgagccgttt
     1021 gcgtgcggcg ctcccaaact ggtcgagcgt cgcgtcgtca ccaagaagat ccatcaggct
     1081 ctgcccatat gcggcggcgg aatccagaag acgcgtgatg gtggtctttc cgatgccccg
     1141 cgttggcgtg ttgatgatcc gcagtagact catgtcgtcg gccggattgg tcatgacgcg
     1201 caaatacgcg agcaagtcct tcacctccgc tcgatcgtag aatcgcatcc cgcccacgat
     1261 gcggtatccg atgttggcgc tccgcagcga ctcttcgatg acccgtgatt gcgcgtgggt
     1321 tcgatagaat accgcgacgt cagacaggcg gtgcccctgc gaaatgagct ctcgaactcc
     1381 ccgtaagacg agccgggcct catcgcgttc gtccatggta cgaacgacca ggatcgcatc
     1441 gccctcttga ttgtcggtcc agagctcctt cggctcgcga tcggtatttc tcgcgatgac
     1501 cgcgttggcg gcccgaagga ttcgccgcgt ggagcgatag ttttgttcga gtttgaccag
     1561 cctcgcgtcg ggaaacgagg cccgaaaatc caggatgttt cggcggtcgg caccgcgcca
     1621 acggtagatg ctctgatcgt cgtcaccgac gacgcaaaga ttttgatgtc cttccgagag
     1681 caaacgaacc agccgaaact gtgcgtggtt ggtgtcttgg aactcgtcga ccaatacgtg
     1741 cgagaagcgg cctacgacgg cattgcggaa ggattcttcg gactcgatgc cgcgcaccgt
     1801 acggtagatc aagtcaccga agtcgagcgc gttggccttg cgaaggcgtt cttcgtaggc
     1861 tgtgtacaca cgacgcacga tctccgacca ggcgtctggc gtttcgatgt cttcgggccc
     1921 ttgaacctct tgcttcgcgc gattgatgcg acctgccatc gcttttggct gatatcgctt
     1981 ctcgtcgaga cccaggtcgc gcaaaacccg cctgacgagc gcgagctggt cttgatcgtc
     2041 gtaaatcgtg aagtcctttg agagcccgaa gctctcacca tggcgccgga gcagtcgggc
     2101 gcaggtcgca tgaaacgtcc cgacctggac ggcggacccc tgctctccga gtaagtcgag
     2161 cagccgctgt cgcatttcgc ctgcagcctt gttggtgaag gtgaccgcga ggatccgcca
     2221 gggcgcgacg ccgtgctcgg agatcaggtg cgccacccta aacgtgatga cccgtgtctt
     2281 gccgcttccg gcaccggcga agaccacgag cggtccgccc tcgtgaacaa ctgcctcgcg
     2341 ctgaggagaa ttcagagagc tcaggtccag caccgcaaaa ccgtagctgc ccgcgcgcgg
     2401 aaaaccagcc gaaatgcctc ctagagcggc ccgggccagc tcgaagactg gccttcaacc
     2461 ccatttgcgc gtacacctcc aagcgtgggt cgcgacggcg actaacccct tggaaacgtc
     2521 gaggatcttg ggtgaatcaa ccgctgtcca gtgcgtccaa acctgaggag tcctcagatg
     2581 agtcctccaa gaagggcgac catgacttgg tacggcgcgt tcaatctgga gattcggcgg
     2641 ctttccgagt gctcttcgac aagtatcatc ggcgggcctt tgccgtggcc atgggcgtgg
     2701 tcaaaaacca agacgacgct ctcgatgccg ttcaagaggc tttcgtcaag gtccataaga
     2761 acatacacaa gttcgaagga agctcgagct tctacacctg gctctatcgt atcgtcatga
     2821 atgcatcgat cgaccacgtc cggcgcacca gcaggcgaaa gagcttggac ttcgacgaac
     2881 gccatttgca cgaagaaagc gacgtcgccg gagacggggc gctcgtaccg aacatcaccg
     2941 acgcaaatcc cggaaaggca gcactgcgcc gggagcttgg aagcgccatc gagacggcgc
     3001 ttcaggggct ccccgagcat cacagagccg tgattgtgct ccgcgagatc gaggggatga
     3061 gctatgaaga gatggcacaa gtactggagg tccccaaggg cacggtcatg agtcgtctgt
     3121 tccacgctag aaaaaagatg caggactccc ttgcccccta tctttccggg gaagcggttt
     3181 ccgaggattg actatggaga gcccgaaacc agaggaccaa aaacttcaga tgttcttcga
     3241 tggggagctc cctcccgagg aagaggcggc gctgcgccgc agcctcgaga gcacacctga
     3301 gggaatggcg cagatccgcg aatgggctca gatccgcgag gcgatgcaaa cggcttcggc
     3361 cgactggtcg ggccagatcg actccgattc gcttttcgct cgaatcgaag ccgaggtcag
     3421 cgcaccggtg attccgatcg agccggcaag acaggcaaag cccccacttc actcggtccc
     3481 cggcggccgc gaacgtcgtg tctggggtgc agtagcaacg gggttcgcgg ccgccgccgc
     3541 gatctttttg acggtcatgg cctggccgga gacgcatcag gacccgagcg gcgtgacggc
     3601 agaagttcgc acccgcggtt ccgaggtgct cgaggtggat ttcgggacga acaccgggac
     3661 gatcttcgag gtcgagggcg gtgctggtga atctcttgcc gtggtctgga tcgacgacgg
     3721 agaggtaggg cttcaatgag tctcattcga tacttggcga tgatcgcagt tttgctggga
     3781 gcttccaacg cctcggccca gggaagctcc tcagtgcgct ccgatcgcga ctcgacctcg
     3841 agtgtgccgg ccgaggtctt ggtcgtactc gccaaggagg agccgggcga aatcgatccc
     3901 gagctgaaga agctgaccgc attgcgcagg ccaccattca attccttccg ctcgatgcag
     3961 atcttgtccc gcccaaagct cacgctcacc ccaggaaaag acgcgttggt ttccttgccc
     4021 aacggccgca gggtgaagct cacccttctc cgggtgatgc ccgacggtcg ctacaaggtg
     4081 aaggcggcaa ttaacagacc ggacaagtcc gactacctac ccctgctgca ggtagtcgcc
     4141 tcggcgggtg acccattctt cgttgcgggg cagacgtatc aaggtggaac gctcgtcgtg
     4201 ggcgtcatcg tcgacccgaa gaagacctcc cagtaggtgc gcgccgacga tccagacgca
     4261 attgaggtcg agggccctcc gcggctggag caccctgcgg aagtcgtgat cgaagccctc
     4321 tccaaagtgg tcacctccaa gcgcctggag cggatccaag acgtcgcgcg atcgcgaacg
     4381 gacaacttgg tcctggtact cgatcgcatc gccgatccgc acaactcgag cgccgtgttg
     4441 agaagtgccg atgcgtttgg ggtgcagagg gttcacgtga tcgttggcga gcagggtttt
     4501 cgagcctcgc gaggtgtttc gaaaggcact caccgatggc tggacgtcgt gcgctacgag
     4561 acggccgacg cctgcgcgcg ccgtctcaga gacgaaaact acgccatttt cgtcgcagcg
     4621 atgggcggcg agacccgcct cgaggacctg cgcgtggcgc ctcgcctcgc ggtggtattc
     4681 ggaaacgagc accgcggcgt ttcaccggaa atgcaggatc tcgccgacgg aacgttctcg
     4741 atccccatgt gcgggttcgt cgaaagcctg aacatctcgg tcgcggccgc gatcaccatg
     4801 cagaccctgg ctggcgacgg ccgagcgcgc cttccggatg cgcggtgccg agagctcgaa
     4861 gcgagattcc taatgaattc agtgaggaac gcgggccaat tgatcgagaa attcatcggg
     4921 aatgattagg cgccccgggc tgtcatgaat tagggctcag gcgcgaagca ggggatgcta
     4981 taggcgccga cggacgagat ggcagggaag cgaacccaaa agaagaagcg caagccgagc
     5041 aagcgcaaga agcggtcaac gggcggtagc tcagctctct tgaagctggt cggggcgatg
     5101 ctcatcgtgg gtgcgctctt gctcatgggc gcctacgcct acttcagccg agacctgccg
     5161 gcagtgagct ccctacggga cttcaagcca ccgcagacga cacgggtcta tgaccgaaat
     5221 ggcaaggtga tcggggagat gttcgccgag cgtcgaacgg tgatcccgat gtcgcaggta
     5281 ccgagaaacg tcgtgttatc ggtgcttgcc gcggaagacg cggacttcta cgagcacgaa
     5341 ggactcgact atcgagggat cctccgcgcg gtgggtcgag acatcgtcgc aggacgagcg
     5401 gcccaaggag gcagtacgat cacccagcag gtggtgaagc tgatgctcct cacaccagaa
     5461 cgaacgctct cgcgcaaagt gcgcgagctg atcttggcgc gccggctgga aaacgagctg
     5521 tcgaaggacg agattctgca tctgtatctg aacctcgtga acttcggcca tgggcgctac
     5581 gggatccaag aagctgcccg ttactacttc gcgaagaacg cgagtgatct cagcttggca
     5641 gagtcggcga tgttggccgg gattccgcag gcgccggcac gtctgtctcc tcgaagccac
     5701 cccgaggctg ccaaacgacg ccaggagtac gttctatcgc aactgctggc gaagcgagag
     5761 acatactggc ccgatttgag cgaagctacg atcgcagctg cgatcaagga gaaagtcgaa
     5821 ccgactgcgt tgcccgaggg tgcgggcaat gcgcccgagg tgctgagcat cgcgcgggca
     5881 gccctgaacg cggccattgg agaggacgcc atgggggaag gcgggtacaa ggtctacacg
     5941 acgatcgatc tgtctctcca gatgcgggcg agagaggccc ttcaacgcgg actacgcgcg
     6001 atcgacgagc ggcaaggcta tcgaggaccg tttcgccgga cgcaacggaa gagcggaccg
     6061 aagccgtcca aagcgcagga gacgctccgc atgggccgaa cgtacgttgc gcgggtgatg
     6121 gacgcggacg acgagaacga gaagatctgg ctcgatgtgg gagggcattc ggcagtcctg
     6181 aggatgcgcg acgcggcacg atacaacccg gacgggcgca aaccgacggg cttcgccaag
     6241 aagggcgacc tgctgcccgt gtcgatcact cagatgggcg acgatgatga tcaagatccg
     6301 gccgtggttc gcctggagcg aggtccggaa ggtgcagtgg ttgtcatcga accgaggtcg
     6361 cgtgccgtgc tcgcactggt cggcggctac gctgcactga gcgggttcaa ccgcgcaacg
     6421 caggccatcc ggcaaccagg gagcactttc aaaccaatcg tatatgcccg cgcgattcag
     6481 tcgcgccgct ttaccgcagc gtcgatggta gtggacgcgc cggcggtata tgacgagtgg
     6541 aagccgcgaa actacgagca atggaactac cagggcgcag tccggctccg cgaagctctc
     6601 gcacgctcga tcaacatggt cgcgatccgg gtggtggaag acatcggcgt tcacgacgtt
     6661 gccgagttcg cacggcaaga cgggatcact accaagctcg aggaagacat ggctcttgcg
     6721 ctcggagcga gcggcgtgag gccggtcgag ctcgccaacg cgtatgcgac gttcgccgcc
     6781 ggtgggcgat gggcccctac tcgggtcgtc acgagaatcg ttgcacccga tggtacccgc
     6841 gtgccgctcg agccggacga gcctgctcgc gacgtgatgt cgccggacga ggcgtacgtg
     6901 attacgagca tgcttcgaag cgtcgtgacc gaagggactg gcgtcccggc ccaacgcttg
     6961 gagcgcgcgg ttgtcggcaa gaccggcacc agcaacgagg cacatgatgc ctggttcgtt
     7021 ggctactcgc cgtcggtcgt agccggagtc tgggtcggat tcgacgatca gcgatcgatt
     7081 gggaagaggg aatccggcac cagaacggcg ctcccgattt ggatcgacgt gatggaggca
     7141 gcggaccaga ccccgaaaga taccgatttc gccatgccga gcggggtgat cgtcgcgcga
     7201 atcgacccgg cgtcgggcct tctggcctac gaaggtcagg aagacgcctt cgaggaggtc
     7261 tttctcaccg gcacggcacc acgcgaggtc gcaaggcctc ccgatgtcgc cgacccgaac
     7321 cgattcttga tggagcagta cgatgaagag gatgccgggg ccgcggccaa tcgacctcga
     7381 ccgtcccgcg aagagcgcgt gctcggcggt gtagctccgc catgaaccga tttggcacgc
     7441 gcgtggtcat tctcttcgcg ctggctgcaa cgtttcccgt cgcactgaca gcgctcgtct
     7501 ggctgggagt ttcgcaatgg ggtgcagcgg gagagtcggg cgaagcggcg ctggcccgga
     7561 tgcaactgaa ccagctcgac gaacgacgga agattgcaat agaccgattc tgtcgcgacg
     7621 acttggtgat cgacacgctt ctcgacgatc gcgcaagaga aacgccgccg gacatcgact
     7681 acgagcgcct ccttcgcggc gcgatgcagg cggcggggct acaggtgctc tgggtattgg
     7741 attcgctgag tggcgaggtg atcgccagcg gtcatcgcaa cggcactctc ggccgcgacg
     7801 gcgcggagct catgcgccag gcgaggacag cgcacgaccg tgcgtttgtt ttttcactcg
     7861 gcgatcaaga acatcagagc ttcgtgctgc gctcgtgctc gatcgctcgg ggaggtgcgg
     7921 aagtcaccgt tgtcggaggg catcgcttct ccgagcttcg tagcctcgat cccaggcagc
     7981 tggtgattgg ccgagaggct ttgaaggggg acgagatcgt cgccgagctt ggggacgctg
     8041 agggcgtggt gcagaacctc gtggtctgga ggctcggggt cgaccggcgc ggcccccctc
     8101 ttctcttgtg ggccgtttcc gttgccgttc tcgcactggg ccttgccttg ctgtttggag
     8161 ggtatctcaa tcgatggttg cgatcatcgg tcgacgagct cacagaagcc gcgactcgag
     8221 tcggtcgcgg tgatttcgac acgaccctga gagatggctt cgggggggcg tttcccgcga
     8281 ctgcaacggc gttcaatcga atgacacgcg acctgcggga cgcgcgggaa cagctcagac
     8341 aaaccgagcg aatcgcagcg tggcaagaca ttgcgaagag cttggcgcac gagctcaaga
     8401 acccattgtc tcccatccgc ttgtcgattg aaacactgcg caaagctcat gaacgttcgc
     8461 acgaggattt cgacgcgatg ttcgatgagt cgacgcggat cattcttcaa gaagtcgatc
     8521 gcctgcgagc aatcgtggat gaattcagtc gattcgcgcg actaccggcg ccgcaacgaa
     8581 gagccaccga cctcggagaa gtccttgccc aggcggcggc gcttcacgcc gagggcgaag
     8641 cgccggtcag gttcgagctc cccgatgctc ccgttgtggc ctcggtcgac agcgatcaga
     8701 tcacacaggt gctgcacaac ttgctgcaga acgcgcgaga tgccgcatgc tctgctcacc
     8761 ctcacggcgg ggcactcgtg cgaatgagtc tcgaaggtcg cgccgacaca gcgtacatcc
     8821 gcgtagaaga caacggaccc ggaattccca ccgaccagat cgaagcgatc ttcgagccct
     8881 actacacgcg gaagcatggc ggcaccggac ttggactcgc gataacgcag aggatcatca
     8941 cggagcacgg aggccggatc gacgtggaat cgcaggcagg acgaacggtg ttcaccgtcg
     9001 tgcttccgcg acggtgactc tcagacggta gggagggagc cgggtcgctg aacctttgga
     9061 cgcgtgagga tggggaggta gcgccaaccg aagcacgcga accccaagca ccaggcgatc
     9121 gcggacaggg ccagcaaaac gcgcgcaccc ggtccgaagg tcgcagccac ccggaagatt
     9181 gcagccagcg tgattagcac gaacgaggca gtcaagatcg ggtggctttc gagcgagcga
     9241 ttggtgtgac cgagggctgc acggctggcg actgcaacga tcatcgtcgt gatggcgccg
     9301 ctcgtcagcg cgtggatgcc cgcggaagcg gggagcaagt cgagcgaggc agcggctagc
     9361 atgaggacgc ccagaggcag ccaagcgaaa ccaacgtgca acacccacac tagaggctcg
     9421 tcgcccgtgt agatcccttg ccatctcgtg agccgcacga acatcaccaa gctcgtcacg
     9481 agcccgagaa tcgccagcca cctagacggc gctcctgcga cgaacgatat ggcaaagccc
     9541 agcatgagcc agctcgcaac gaggtcgatg cgctcgaacg gcggcggtag tctcaccggc
     9601 gcgtgcttcc gatcgccaag ccgacgattg atccagttcc gcgtgaaccc aggaatgatg
     9661 cggccaccga tcagattgac cagcagcacg acgagccaaa ggccggccaa gagagcgttt
     9721 ctggcccaac tcgcgttgcg ggcgacgccc agaaagaaga ccgcgttgct gaccggaagg
     9781 agtccgagga tcagaagcac cttgtagttg cgctggctgc gcgccgtaat cacctcacgg
     9841 ctcatcaacg cgaacagcag cgcgccatac ccgagatccg ctgcgcacgc gggcaccagc
     9901 atggtgctct cggaggatgc gaaaagtagc cgcgccagaa cccaaagcgc gctgagtacg
     9961 atgagaagta cgccggatac cgggggacgc ttggtccagg tcgccaccgc ggtcagcgca
    10021 aagcccccga tggctgcgcc ggaaaagccg tagatcatct cgtgcgcgtg ccaccaggat
    10081 ggcgtcccaa gcgtattggg catgggcagg tgcccgagcc aggcggccgc ccagaggggc
    10141 accacgatga tcgcgtagag cgtggccagc atgaagagcg gcctgaatgc gtagctgaac
    10201 aagacagtca cgaaaccacc ggcgacgatg acgacacccc tactgcgtct ccattccgta
    10261 cgcgcgcaat ttgcgttgca gagttcgccg gtcgattccc aaggcagcgg cggccttggt
    10321 gatgtttccg tctttctctc ggagtacgcg ctcgatgtga tcacgctcga cgtctgccag
    10381 gctgactcgg ccggactggt ccgatgaaga aggcagcacc gattctcgag cgttgccgaa
    10441 aacgctggcg gcaatcatgc gtctcaggag ctcaccgtcc atggtgtcgg agatcgcgaa
    10501 gagcgaggcg cggcgaagca cgttgagtag ctcgcgcacg ttgccaggcc aatcgtattc
    10561 tctaagggct tccaacgact cggccccgag ctggatgccg ctctttccga ggcgcgcgag
    10621 caagtgaagg gtcaacggtt cgatgtcggc cagtcgttcg cgcaaaggtc gaatcagaac
    10681 gggaaacacc tgaagtcgaa aatagagatc ctctcgaaac ctgcctgctt gagtctcagt
    10741 ccacaggtcc cggttggtcg ccgcgacgat acgcacgtcg acctcggttt ctccagaatc
    10801 gccgactggg cgaatggact tggtttcgat ggcgcgaagg agcttcgcct ggctctccaa
    10861 aggaagctcc gcgagctcat cgatgaacaa ggttccaccg tcggcttcgc ggaacaagcc
    10921 cgccctgtcg ttttgcgcgc cggtgaacgc gccacgcttg tggccgaaca gctcgctctc
    10981 gaatagctct cttggaacac aggcagcgtt catcgctacg aagggccgtg cggcacgttg
    11041 actttgtgca tgaatctgcc gagcgaccac ctccttgcct gtgccggttt ctccgaacac
    11101 caggacggtt tcattggtcg gggcgaccgt gcggatgaag gcacggacct cctccatcgc
    11161 tgggctggtt ccgaccagtg gagtggtttc cggagtcgat ccggactccg ttcgcccttt
    11221 ttctaccaaa gcttgctgga ggagcggcct cagcatgttg gcatccacgg gcttctccag
    11281 aaaagagaac gctccgagct gcatggcctc gacggctgtc ttcactgtac cgtgtcctgt
    11341 caggacgatg actcgcgtgg ggatcggtcc gcagcgccgc aggacctcga gccctcctat
    11401 ccccttcatt ttcaggtcga ggagggcaat gtctggtgca gcgctgttgg agagcacttc
    11461 aaccgcttct tcaccactgg ccgcctccga cacttcgtgt ccgcttcggc gtagcgcttt
    11521 cgccatcgcg aagcgaaacg ccgcatcgtc gtcgatgatt agcacgctgc cgttcattgc
    11581 tagggcgcac ggtgatgcac cccaggctca tttgcaagcg tctcggctcc aagcgaaagc
    11641 cgtacgcggg cgctagcacc accttcggct ctgttctcga tcgtgaggtc accacccatc
    11701 gtctgcacca gcatgtatga agcatagagg ccgagaccgg tcccctcgcc tgggggcttc
    11761 gtggtcgcga atggctcgaa cagacgctct gcgatgtctg gatgaatccc gacgccttgg
    11821 tcatcgacga tgatctcgac cgaggatcca cgtcgctccg cgtgcaccca aacgctcatt
    11881 cctcctgctt cccggttcga atcgagcgcg ttttgaagca agttgacgag cacctgcaag
    11941 agacggtcgg gatcggccac gacgtcgagg ttctcgatcg agtcgccaat gtggagctcg
    12001 ggaccaccct cgcgcacccc agcaaaaacg agggcccgtg cacgctctac gacgggaagc
    12061 agcggggctg aggtgttcaa gcggagacgc ggcagcgtgc cgccggtcag caaggagtgc
    12121 gtaatgcgcc cgagtcgacc tgtctcgtcg cgaatgaggg ccgccgattc tgcgaggtcg
    12181 gactgcaagg cttcgtcacc accgagcgat cgaattgcct ctcgcatatc ggccgccaag
    12241 gttcgaatcg tcgccaacgg tgtgttgagc tcgtgcgcga cgccttgggc gacacggccg
    12301 aggctggcca agcgctcggc atggagtagt tggcgttcag cgtgcagaat ctgggtgcga
    12361 tccacgtaca ggttcatgat gcgcgccggt tcgtcgtccc tggaacccaa tggaaacgaa
    12421 agcatctcgt aagatcggtc acccacgctg aatccgcagc gcccctcggc ccgatcatct
    12481 cgcgctcgcc aaacgggaca aagacccgcg ggctgagttg tgcattcgcg tttcgcgatc
    12541 gggcactccc acatgccctc cgcgttgggg agcggggcga gctcgtccgc caagcgattc
    12601 cgccactcga tgcggccatc cggggacacc acctcgagac ccgcttcgag ctcgtcgagc
    12661 gtgttggaga tgacctcgta ctcgagctca gcctggtcgc gcgctcgcgc cagagcacgc
    12721 tctcggtctc ggagctctcg aacggcgtgc aggaccacgt acgcggccgc ccctaccgtc
    12781 gtcatgaatg ccgccacgtc cccgatggtg tcccagggcg gcgccgggtc ccatgaaccg
    12841 atcttcaggg cgggcaccca ctctgacagc gcgagaaaac cagcgccggc gaaggcggcc
    12901 gccgtcgcaa ccagtgtggc gccgggtccc ccgagaatgc cgatcaaggc gaggtgaaac
    12961 acatagaaac caatgaacgg agacgatatg cccccggccg cccagagaac cacggtgagc
    13021 atgacgagat cgacgagcgc ttgagaaact gcggctctct tgccgggagc gggagcccgt
    13081 cgcgtcaaaa gaaagttgta gagacttcct acgacaacga cgcccaacaa gactggccag
    13141 gcgactccgg ggaagtaacc tgcgagtacc aggccagtcg cgaacaggac gcccgccatc
    13201 gcgacccatc tcaagttgat gagccaccgg atgcgatcgg cagtgaaatc gtctcgctcg
    13261 agcgaatcga agaaaacgct ggaacgagtg ggttccgagg ccatcatgag ttcctgggtt
    13321 ttggggtcga tcgacttcgc cggccagggt acaacgaggg gaccggtcga tggcaagtga
    13381 atccagggag agaaagatgg tcatcaccat cctactgacg tcgacactgc tcacgatgct
    13441 cggaatcgct ggtgtgctga gaggggccta cgacctatcc ctggctccgc tccccgagga
    13501 cgttcgtgag ctctacacgc cggatgtaga ggtcacggaa gccgaggtgc aacttctcga
    13561 ggcccaagtt accaacccct atcgccggcc gatggccgcc gccaacgtgg tggtttcggc
    13621 gctggtgttg atcgggagct tcatgctgag ctggcgacgc agactggcgc aatggtggat
    13681 caagcaggca gtggtggcga agctgatctg gatcgtcgcc tacacggttt cactcatctc
    13741 gcacatcaag ctgagcttcg ttcccctgcc gatcgagcaa cccggtgccg ccctgcagga
    13801 agtcatggcg ggggtggttc tcgtgagcac gttcagtgca gcactacacc tggcggcggc
    13861 ctatagggct acgcgacccg acattcgtga gttcatcgag acgacagcca agccctgagc
    13921 cctagaagtt gatgcgccgc atgatcgcgc gcggcagcgc tcgaatcacg gccatcacga
    13981 gcccccatat cgcaggggcg tatacgacgg gcgttccgcg atcgactgct ctgagcactc
    14041 gcttggccac ggcctccggc tcgccagcaa agggcggagg ttcgaggccc gcagtcatgc
    14101 tcgtcttcac gaacccaggc ttggccgtga tcacacgcag accattcgcc cgatgcttgt
    14161 gatccaggcc ctcgagatag tgggtgagcc cggccttggc tgccccgtat aggatgatcg
    14221 gcttgcgtcc ccgctcgccc gcgacggagc tgaatgcgca aagggtccct ccgccttccg
    14281 agaggagggc ttgcttcgcc gcctcgcaga acaatacggt gttcgtgaag tcgatgcgaa
    14341 gcagacgttc ggtgagctcg agatcggctt cgagctcgtc ctgggtcgca aatacgccag
    14401 ccgtgacaat cactgcatcc aagcccccaa gcgattcgcg tgcagcgtca atcgcaccgg
    14461 caaacatccc gggctcttcg aggtcgcagc gcacgcaagc cacgatgcca aaggcgccac
    14521 gtacgctcag gtcggctgcg cttcgctcga gatcgacact atctcgaccg agaaggcaga
    14581 tcgcatcgcc acgctccgcc atcaatcgcg caagagacct tcccatgcct ttggtcgccc
    14641 cgagaatcgc cgctttcatg tcagtccccc agcaagcgca cggactgcgc gcttcgaaat
    14701 cgtcgatccg gatcccagcg gtcgcggacg gcgagaaact catcgagacg gctgccttcc
    14761 attgcaacaa aatgctcccg gcgcgtgaac gagtccttcg cgaggtagat gcgcccgcct
    14821 tcctgcagga cctgctcatt gagtgcatcg acgagagctt gtgtgtcgtc tcggatcgct
    14881 atgtcgaggg ccagagtgat gcccgggcgt gggaacgaca gaatgccgcg gctttcacgc
    14941 ccgaaatctt tgatgacgca gagaaatgac gcgccaccgc gagccgtcaa aacctcgaga
    15001 acgcggcgag cggagctcct acccgcttca cgcggcagta cgcattgata ctgtgtgaac
    15061 cctcgacgac cgtacattcg gttccaggcg cgaatcgcat cgagcgggta gtaagacgac
    15121 tcgtaattga cgataccact cgaccgaggg cgccactgct tgcgatagta gagctcgttg
    15181 aatgtccgaa tactgaggcc gctcaaagcc cacgacggcc agtcgaaacg taggctcggt
    15241 tgaaagcccg gcttgggcgg acgtaagggc gcctcctcgg cttctgccca gcgagcggtc
    15301 atgagaatcc ccctgcccat gttctctccg cccgccagac agtcgatcca gcccatggtg
    15361 tacggccact cgggtgctac ctcttccaga gcgtcttgga attcatcgat gtcgtggatc
    15421 cgccggttct cttgccagag ccatcgagaa gggatgcggt gcatcccgaa ctccacctcg
    15481 agaatatgac cggtgagacc cattccaccg atcgtcgctc ggaataggtc tgggtgttgc
    15541 tccggggaac actcgagcac tcgaccgtcc gcgacacgca ttttcagagc cgtgacgtgg
    15601 tggccgaagt tcccttcctt gtgctggttc tttccgtgga tgtcagcagc caccattccg
    15661 ccgagggtga cgaacttggt ccccggtgag acaggggtga agtagccgcg cggcatcaag
    15721 aggcgattca gctcgctgag ggcaagccca gactcggcgc ggaagagccc tgtctcctcg
    15781 tcgaatccca gaattcgatc tgcgaacgtg gtgttcagga cgtcagggcg atcgtctggc
    15841 ggcagcgacg cgtcgccgta cgaacggccg agccctcgcg acaaatgagc gtctgcgctc
    15901 atgcgctcga tgtcttcgcc gaaatgctcg cgcccgacag ccgagtgccg gccccatccg
    15961 gagagagtcg aaagcggtgg ctcacgcatg gctaagggac tgtagcgcga cgaggaccag
    16021 taccctaacg cccgcgcttg ggcatgagcg cgttgcggag ctcatcggca cgatcggtcc
    16081 gctcccaagt gaatgtcttt tcggtgcgac cgaaatggcc gtaggccgca gttggcacgt
    16141 agatggggcg cagaaggtcg agtgtctcga cgatggcagc cggccggaaa tcgaacagct
    16201 tggcgaccgc tttgcctatc ttctcgtcag gcactttacc ggtgccaaac gtcgtcacgt
    16261 atacgcccat gggccttgct acgccgatgg cataggccac ctggacctca catcgagacg
    16321 cgaccttgga ggccacgatg ttcttggcga catatctggc aaagtacgcc gcgctgcgat
    16381 cgactttgga ggggtccttc ccactgaatg ccccgccacc atgccggccc atcccgccat
    16441 aggtgtcgac gatgatcttg cgaccggtca gacctgcatc gccgtagggg cccccaacga
    16501 cgaagcggcc agttggattg atgtgaatct tggtcttgtt gtcgatcatc tttttcggaa
    16561 gtgtcggcgt gatgacctcc tcgatgatcg cctctctgag ggcgcgctgc ttgacgctcg
    16621 cagcgtgctg cgtcgaaacg acgatggcgt cgaagcgctt cggcttgtcg tcctcgtact
    16681 cgaccgtgac ctgagtcttg ccgtccgggc gtagcccttc gatcacgttc ttcttgcgaa
    16741 cctgcgtcag cttgcgggcc aacttgtgcg cgaggtcgat gggcattggc atcagctggg
    16801 cggtctcgtc gactgcgtag ccaaacatca aaccctggtc gccggctcct tgctctttgg
    16861 ttgcgctcga gttgacgccc cgcgcaatgt caggcgactg ctgctcgacc gcagcaagaa
    16921 ccccgcaagt ctccgcatcg aagcccatcg aagaatcggt gtagccgatc tcggtgatcg
    16981 ccttgcggac gattttcggg atctcgatga ccgccttcga ggtaacctcg ccaccgcaaa
    17041 cgacgaaacc ggttttgacc atggtttcca ccgcaacgcg cgacttcgga tcctgttcga
    17101 ggtacgcgtc gagaagggaa tcgctgatct tgtcgcagat tttgtctggg tgaccttcgg
    17161 tcactgactc ggatgtgaac agatacttgc tcatattggc gttgcctctt atccgcgacc
    17221 gccccgagag tcaatcagac ccagacgggt cgggcgccat atccgaaaaa cagccgggct
    17281 aaccgtcgcc taggctgtcc tttcggtgtt taaccagtga tcagagcgtc gggatctcgg
    17341 tgccataggt ggcgagaacc ttcaacccat aggggtcgca gtcaccttcg aattcgcggg
    17401 actcgacccc gaccagaacc gtcccgacct gcgagctgac gttgctgtcg cacttccgct
    17461 cgatgccgat ccaatcgccc tcgacgatgc agaactcgat cccgtcaatc ttctcggtcg
    17521 agcccgagca cttgtcgaga ccatcgaacc cgtcggggca gagataggtc atggaaagga
    17581 ccctcttgct cttgccattg taggcttgag cgatgatggg agttcgagca tcgagaacgc
    17641 gcaatgcata gtagtccacg tccctggaat cgtggagcgt agcttgaacg tcgacgctcc
    17701 atgccgcttg catgcagttg attccgaggc tctcgccgca aatgagagag aacgtctcga
    17761 acgaatcgtt gtactcgaaa ctgtcctcgc agacgcaacg tccactctca cagtgatagc
    17821 ccggttcgaa gccgttgtcc gcgcaggttc cgcatagggt gggtgagcca caaatgctcg
    17881 tgcttccgca ctccactccc tgagcctcgc acgtgcaagc accgagacat tggtagcgga
    17941 gattgtcgga caggacacag tcctctccgt taccgcaggc agggcattgt gtgaatcccc
    18001 cgcaaccgtt ggggagccca ccaggcggcg agcattcgtt ctgcgcacat gggtagctcg
    18061 gctcgcaaca ctcgccgccg acgcagacct ctccatcgcg gcagctgccg ccacagccac
    18121 cgccacagtc gactcccgga ggacagacac actcgttctg aacgcattcg aagccatcac
    18181 ccaggcagtc gccgcagaag atcgcttccg cctgaccacc acatcgggtc gggacggccc
    18241 cgcactccgc gccgcctcca tagttggcgc agctgttctc ctcgcaaccg caggcgaagt
    18301 tctccccatt cgctccgcaa acggtgccgg ccggacaact gccacattcg acggagccat
    18361 cgcaaccatc tggaagagcg ccgcattcga tggaaatgcc tagggacgcg aaatcggaga
    18421 tcacttcgtc gcaggtcttg ggaacacatt ccggttcctt ggtgtcgtag caacggctat
    18481 cgctgctcca gcagaaatag tccgacggac agtccgaagg ctgaccacag ccgaatatac
    18541 cgttggggat cgtgaggtcg caccccccca aagcgacgag cgcgacgatc aacgcaaagc
    18601 gcaaacgcat cagaatcttc ctttcacgga gatgccggtc gggccgatgc tgatctcggg
    18661 aagcgcctct tcgtgaccac ccgtgagttg ccagatgacc ccaccgacca ggccagcgcc
    18721 gccgacgctc aggagggcga tgcctgcttt ggagagtcgc ggaccgcggt cggccacatc
    18781 ttgaaacgtg gaatagctcg atccgggcgg ggcacttgtg acctgattct cgtttcgctg
    18841 ccccatcccg aggagcaccg cgccaccaag gactgctgct ccactcatgc cgatggtgat
    18901 ccaaggacct ggaccgggtc caacgtgctg cgtgagcggt cgctcggccc tgaccttggc
    18961 ggcagcctcg accgcggcct tccgcgcctc gatgtcggcc tcaatctcgg ctcgtcgacg
    19021 ctcttcgatg gccgcacgta agacctcgat ccgcccctcc acgtgctccc tgtccgcgga
    19081 gtccggtcgc gcttggagat aggcttcata ggcttcgagt gcctcttcat ctcggcgcat
    19141 gcgatctgaa acggttgcta cgttgaaaag aagatccggg cttcccgtta gctcgtaggc
    19201 gcgttggaag cttgcgagcg ccgtatcgaa ctcaccgtcc gcgtaggctt gcgttccgat
    19261 cgagaaggcc ttcttcgcct cagcaatgcg cgcgtcgcgc gactcagtac tctgtgcgcg
    19321 aacgccgact gcggcgacca ggcatgaaag tgaaactact agtacaagaa ggacacgcat
    19381 ctttcccccg aaagcaacga cgcgcgcgca gcgcgtccct acaaactagc agcgggaaag
    19441 agtggatgct cattcgtgag cgatgtacgg ggcggtgttc cgagagatgc gggtgtcccc
    19501 tctccattct tcaccggcgt gcgggtagtc caaattgtaa tgcaggccac gactctcacg
    19561 gcgagcgctc gcgcaacgga tgatcagttc agccacgtcg gcgatgtttc gcagctccag
    19621 catgtcgcgg ttcacgaggt gagcccagta gtactcgcgg atctcctctt gcagcatcga
    19681 aatgcgtcgt gccgcacgtc gtaggcgtcg atcggtgcga acaatgccca catagttcca
    19741 catgaatcga cgtatctcgt cccagttctg ggagacgacg acggcttcgt cgctcggcac
    19801 cgcctcgccg acgtcccagt cggccacata ttcgtcggcc acttcgggca gcaagctcaa
    19861 agcctttcta gcagcgatgg cggtgcgatg accgtaaacg agcccttcaa gaagtgaatt
    19921 ggaggccaac cggtttgcgc cgtgcagacc cgtgcatgtc acctcgccta ccgcccagag
    19981 gccgggaagg gtcgagcagc cctcgtcgtc gacgacgacg cctccgcaca tgtagtgggc
    20041 tgcggggacc acgggaatcg gttctttcgt catgtcgatg ccatattcta ggcagcgcgc
    20101 gcggatgttt ggaaaccttt cgagaatgaa atccgccggc tcatgggtca tgtcgagata
    20161 cacgcagtcg tgtcctctca gcttcatctc atagtcgatc gcgcgtgcaa cgacgtcgcg
    20221 cggcgcgagg tctttcatgt cgtggtggcg ctccatgaac gcctcaccgc tccgca
//
LOCUS       DASRZF010000256         6354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1220573, whole genome shotgun sequence.
ACCESSION   DASRZF010000256 DASRZF010000000
VERSION     DASRZF010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 6354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6354
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1220573"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..271)
                     /locus_tag="VFG22_11390"
     CDS             complement(116..271)
                     /locus_tag="VFG22_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416890.1"
                     /translation="MRAQSKWTATGIVLWLIAAVAATLLVCSETAPPPEAAATPQGDE
                     VEVGLVE"
     gene            340..1581
                     /locus_tag="VFG22_11395"
     CDS             340..1581
                     /locus_tag="VFG22_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416891.1"
                     /translation="MRNFLTVLVLLLTWGSAALAENRSGKVDATVARLGSLDPAERAL
                     LVPYLAQGPVVLTEFQNRQTDLPAVIYAARIEAPAETVAGVIGNPKEYPRFMRALTEV
                     DVKSINGQMMAFDWTWGVTLFSLSGQNVMTTYPGDSEQGYRFDIRTLGGDLGIGRVMW
                     RVYPEGPKRSLVVFSSRLDMRDANYLTRQLASEGSAVNRTINVAVAMVTLLEVKAEAE
                     RRAGTFFTGKFPSKPLHRPQVDMKALRGLLGRGDLVFLDLDGDTLQSVAVVGRSGASV
                     ERVRRVMLDPEEFGKSLLHGTCAEVVERSAAGTRFEWEIPIPLLHVGGEMMLRPSSSV
                     IAVDGISGTLSKGQWRFDTYRYPGGEAGVIAWASFDPADSPKLIRKLIAGNTHFSHGF
                     VAATQLAVMRSLRTRVQLLGR"
     gene            complement(1578..1769)
                     /locus_tag="VFG22_11400"
     CDS             complement(1578..1769)
                     /locus_tag="VFG22_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416892.1"
                     /translation="MTRSGLAFLLLFGLWPLAAGGCSLIVDFDPKLLLDAGVDAGMDA
                     GPDAGPDGSVDAAVAAEER"
     gene            complement(1771..2751)
                     /locus_tag="VFG22_11405"
     CDS             complement(1771..2751)
                     /locus_tag="VFG22_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416893.1"
                     /translation="MKGFLLSLLLFSLAAQADAQVIPEPEEAERLPTDAELAELLADK
                     GTTAADAYEIGVRFFQAKNYESAETAWFRAHELGGDPKLLVAIADTRQRREDEPGAVA
                     MLMQYLAERPDAPDRPSIEARIATLLQSPAVLIVRSLEPGHAILLDGAPVEKKTPAEL
                     EVEPGMHTVLVFGEGKQVGEETVQVGYGEVKELDFTPETSSQVIVEDSKPSELEARLA
                     SEEQDTTIRRAVISTGSIAAASLVTGTALGVLALKKQQSYDSRPSTATADQGERLALF
                     ADLAFGITVLAAITSFTLFMTHKNKRNKERETAHLRIDARGAGATATLRF"
     gene            complement(2757..3167)
                     /locus_tag="VFG22_11410"
     CDS             complement(2757..3167)
                     /locus_tag="VFG22_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416894.1"
                     /translation="MRNTKSALTLLASASLVFLVACVGGVGTTCFKDDQCDGSFICCH
                     LGSPFTQGRCETQQVCDELIGSTGGFGGSGGFGSDAGTAGTGGTGGMAGAGGTAGTGG
                     MGGLGGTGGLGGAGGLGGAGGLGGGGTGGTGGGV"
     gene            3254..4192
                     /gene="era"
                     /locus_tag="VFG22_11415"
     CDS             3254..4192
                     /gene="era"
                     /locus_tag="VFG22_11415"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HET6416895.1"
                     /translation="MPQTENHADAPRAGRCAIVGRPNVGKSTLLNAILGQKLVIATPR
                     PGTTRSCVLGVFASDDPPTQVAFVDTPGLERPKGALGRVLIDSAKQGLMDCDSVVFVT
                     EAPKAGASVGVNDKDLTVLNALEASNVPVILAINKVDQLKAKEKLFPFIEAYQKKREL
                     SAIVPISATRGTNLDALLAEIRLTLPEGILYDPDFLTDRPERFFVSELIREAAMLNTR
                     QEVPYGIACEIESYREENGMLRIEGNLIVEKQSHKAIVIGNQGETIKKISTEAREQIE
                     AFLQRKIYLRLFVKAVPGWTRDPVKARELGIKEHQG"
     gene            4189..5556
                     /gene="der"
                     /locus_tag="VFG22_11420"
     CDS             4189..5556
                     /gene="der"
                     /locus_tag="VFG22_11420"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HET6416896.1"
                     /translation="MSGRRRAHARRPAGSIINRPLVAIVGRPNVGKSTLFNRLAGQHI
                     AIVEDIPGVTRDRHYADAFALGREIVLIDTGGFDPDSDDPMKAGIASQVRLALEECDL
                     VVCVVDATVDPLPADREAVALLRDAGKPVIFVANKADSREKEHDAVSYYELGIDSIMP
                     ISALHGRGIGDLEEEIANQLPEPVEIEDPELDVPRVAIIGRPNAGKSSLVNQLCGEER
                     QLVDNRPGTTVDSVDTLIDRDGNRLILIDTAGIRRKRSVKKQGVEGLSVLQAIRSIER
                     SHVVVLMIDGEAGVGEQDAKIAGLAHDRGRALVLALNKTDLMSRDEQKKAMDRTREVL
                     AFAPWAPLVSVSALEGKGINALLGRVVDAVEEHRKRIGTAEMNRFFEEVLDKHPPPTM
                     HHRPVRLYYITQASVAPPTFVVMTNSPDDVHFSYQRYVVNQIRQRFGFKGTPIRVRYR
                     GKKKK"
     gene            complement(5557..>6354)
                     /locus_tag="VFG22_11425"
     CDS             complement(5557..>6354)
                     /locus_tag="VFG22_11425"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET6416897.1"
                     /translation="ESVCIPMGRGRYTLCVSSQVGCKMGCTFCETGRMGLLRHLRTDE
                     IIAQLLLARHRLRWPIRNIVFMGMGEALDNAHNVFHALRVLNDEAGLAIGQERLTVCT
                     VGHVEGIRMLDQQGLKRLNLSISLNAPNDSLRSELMPINRRTDLAELQNTLAGYRPRR
                     NFVLGVNYCLLPGINDTQSDARAIAAFCEPLGRVLLNLIPYNPGSQPLTRAPEEREVE
                     RFIGWLRAEGLPVRERKTKGRSVMAACGQLGNPSARRARKTLPTLSA"
BASE COUNT         1237 a         1950 c         2026 g         1141 t
ORIGIN      
        1 tcgatgtctg cggagtcgat gaaaatctgc atggggctag taaagagcga tggcctcgca
       61 gggtcaagca gagcctcatc gaagccaaat cgcgaccgag aaaccccgga tccactcact
      121 ccacgagccc gacctcgacc tcatcgccct gaggggtggc ggcagcctcg ggtggaggcg
      181 ccgtttccga gcaaaccaac agggtcgcgg cgacggcggc gatcagccac aatacgattc
      241 cggttgcggt ccatttgctc tgcgcgcgca cggccaggct ctcagaggac cgttttgcaa
      301 cgggattgaa gaacaagcaa ccgaggtcgt ccaagcgaca tgcggaattt tcttacagtt
      361 ttagtcctct tgctgacgtg gggcagcgcc gcgctggccg agaaccgaag cggcaaggtc
      421 gacgcgaccg tcgcgcgcct gggcagcctc gaccccgcgg agcgggcgct gctcgtaccc
      481 tatctggcgc aaggccccgt cgtgctgact gagtttcaga atcgccaaac ggatttgcct
      541 gcggtcatct atgcggcgcg catcgaagct ccggcggaga ccgtggcggg ggtcatcggg
      601 aatccaaagg agtatccgcg gttcatgcgt gcattgaccg aggtcgatgt gaagtcgatc
      661 aacggacaaa tgatggcctt tgactggacc tggggggtca cgctcttctc gctgtcgggc
      721 cagaacgtga tgacgacgta tccaggtgat agcgagcagg gttaccgctt cgacattcgg
      781 acactcgggg gcgatttggg aatcggacgc gtgatgtggc gtgtctatcc cgaaggtccc
      841 aagaggtcgc tcgtggtgtt ttcgtcgcga ctcgacatgc gcgacgccaa ctacctcaca
      901 cgccagctcg cttcggaagg cagcgccgtc aaccgaacca tcaacgtggc cgtagccatg
      961 gtaacgctcc tcgaggtgaa ggcggaggcg gagcgacgcg ccggaacgtt cttcacgggg
     1021 aagttcccga gcaagccact ccatcgtcct caggtggaca tgaaggcgct tcggggtctc
     1081 ttgggtcgag gggacctggt gttcttggac ttggacggcg atacgcttca gagcgtcgcg
     1141 gtcgtcggac gttccggggc aagcgtagag cgtgtccgcc gagtgatgct cgatccggag
     1201 gagtttggaa aatcgctgct gcacggcacc tgcgccgaag tggtcgagcg cagcgccgcg
     1261 ggcacccgct tcgagtggga aatcccgatt cccttgcttc acgtgggcgg cgagatgatg
     1321 ctgcggccga gttcttcggt gatcgcggtg gatggcatct ccggcacgct ttccaagggt
     1381 cagtggcgct tcgatacgta tcgctacccc ggcggcgaag cgggcgtcat cgcctgggcg
     1441 agcttcgatc cggccgattc cccgaagctc attcgaaagc tgatcgccgg caatacgcat
     1501 ttttcacatg gtttcgtcgc agcgacccag ctcgcggtga tgcgctcgct tcgcactcgg
     1561 gtgcaactac tgggccgtta gcgctcttcg gctgcgaccg ctgcatcgac ggacccgtcg
     1621 gggccagcgt ctggacccgc gtccataccc gcatcgaccc ccgcatctag caatagcttc
     1681 gggtcgaagt cgacgatcag cgagcatcca cccgcggcta gcggccagag tccgaaaagg
     1741 agcagaaatg cgagaccgga tcgtgtcatc tcagaaccgg agtgtggcgg tcgccccagc
     1801 gccccgtgcg tcgatgcgca aatgcgcggt ctcacgctcc ttatttcgtt tgttcttgtg
     1861 ggtcatgaag agcgtgaacg aggtgatcgc agccaagacc gtgatcccga acgccaggtc
     1921 ggcaaagagc gcgagccgtt cgccctggtc tgcggtagcc gtgctggggc gcgagtcgta
     1981 gctctgctgt ttcttgaggg ctagaacgcc gagggccgtg cccgtcacca acgaagcggc
     2041 tgcaatgctg ccggtcgaga tcacggcgcg gcgaatcgtc gtgtcttgct cttccgaggc
     2101 gagccgagcc tcgagctcgg atggcttgct gtcctcgacg atgacttggc tcgatgtttc
     2161 gggagtgaaa tcgagctcct tcacctcgcc atatccgact tgaacggttt cctcgccaac
     2221 ctgcttgccc tcgccgaaaa ccagcacggt atgcatcccg ggttcgacct cgagctcggc
     2281 gggggtcttc ttctccacgg gggcaccatc caagaggatg gcatgacccg gctccaggga
     2341 gcgcacgatc aagacggcgg gactctgcaa aagggtcgcg atgcgggcct cgatggacgg
     2401 ccgatcgggc gcatctggcc gctccgccaa gtactgcatg agcatcgcaa cagcgcccgg
     2461 ctcgtcttcg cgacgttgcc gggtgtccgc aatggcgacg agtagcttgg ggtcgccacc
     2521 gagctcgtgc gcacggaacc aggcggtctc ggccgattcg tagttcttcg cttggaagaa
     2581 gcgaacccct atttcatacg cgtcggcggc cgtcgtgccc ttgtcggcca gaagctcggc
     2641 gagctccgcg tcagtgggca atcgctccgc ttcttcgggc tctggaatca cctgcgcatc
     2701 cgcctgcgct gcgagactga aaagcagcag tgaaagcagg aagcctttca tcgagcctag
     2761 acgcctccgc cggtgccgcc ggtgcctcca ccgccgaggc cgcccgcgcc gccgaggccg
     2821 cccgcgccgc cgaggccgcc ggtgccgccg aggccgccca tgccgcccgt gccagccgtg
     2881 ccgcccgcgc cagccatgcc gccggtgccg ccagtacctg ccgtgccagc gtcactcccg
     2941 aagcctccgg aacctccgaa acctcccgtg ctgcctatga gctcgtcgca gacctgctgg
     3001 gtttcgcaac gtccctgggt gaatgggcta cccaagtggc agcaaatgaa ggacccgtcg
     3061 cactggtcat ccttgaagca agtggtgcct acgccaccta cgcaggcgac caaaaacacg
     3121 agagacgcgg aagcaagcaa ggtgagagcg gacttggtat ttcgcatggg cgcgccgagc
     3181 atagctcaga ggctctggag gggccacgct gccggctcga tcgcgcgcag cccccggcta
     3241 cactgcggcc tccatgcccc agaccgagaa tcatgccgat gcgcctcgcg ccggccgatg
     3301 cgcgatcgtc ggacgcccga atgttgggaa gtcgacgctg ctcaatgcga tcctcggaca
     3361 gaagctcgtg atcgccacac cgagaccagg cacgacgcgc tcttgcgtcc tcggggtttt
     3421 cgcgtccgat gatcccccga cgcaggtggc cttcgtggac acgccaggac tcgaaagacc
     3481 caaaggagcg ttgggccgtg ttctcatcga cagcgccaag caggggctca tggactgcga
     3541 ctccgtggtg ttcgtcaccg aagcgccgaa agcaggcgcg tcggttggcg taaacgacaa
     3601 agacctaacg gtcctgaacg ctctcgaggc ctcgaacgtg ccggtgatcc tcgcgatcaa
     3661 caaggtcgac cagctcaagg ccaaagagaa gctgtttccc ttcatcgagg catatcaaaa
     3721 gaagcgcgag ctgagcgcga tcgtgccgat ctccgcgacc cgcggaacca atctcgatgc
     3781 cctgctcgcg gagattcgtc tcacgcttcc agagggcatt ctctatgatc ccgatttcct
     3841 caccgatagg cccgagcgat tcttcgtgag cgagctgatt cgcgaagccg cgatgctcaa
     3901 cacgcgacaa gaggtgcctt acggaattgc atgcgagatc gaaagctatc gtgaggagaa
     3961 cgggatgctt cggatcgaag gcaacctcat cgtcgagaag cagagccaca aggcgatcgt
     4021 gatcggcaac caaggcgaga caatcaagaa gatcagcacc gaagcgcgtg agcagatcga
     4081 agcattcctc caacgaaaga tttatttgcg cctttttgtg aaggccgttc ccggttggac
     4141 tcgcgacccc gtcaaggcgc gcgagctcgg gatcaaggag catcagggat gagcggacgg
     4201 cggcgagccc atgcgcggcg gcctgcgggc tcgatcatca atcgtccgct cgtcgcgatc
     4261 gtcgggcggc ccaacgtggg caagagcacg ctcttcaacc gattggctgg ccagcacatc
     4321 gccatcgtcg aagacatccc gggcgtgacg cgcgaccgcc actacgcgga cgcctttgcg
     4381 ctaggtcgag agattgtgct gatcgacacc ggagggtttg atcccgacag cgacgaccca
     4441 atgaaggccg gaattgcgag tcaggttcga ttggcgctgg aggaatgcga cctggtcgtc
     4501 tgcgtcgtcg acgcgaccgt cgacccgttg cccgcggatc gcgaggcggt tgctctcctt
     4561 cgggacgcag gaaagccggt cattttcgtg gccaacaagg cggactcgag ggagaaagag
     4621 cacgacgcgg tctcttacta cgagctcggg atcgactcga tcatgccaat ctcggcactt
     4681 catggacgtg gcatcggcga cctcgaggag gagatcgcca atcagcttcc cgaacccgtg
     4741 gagatcgagg acccggagct cgacgtcccg cgggtcgcga tcatcgggcg accgaatgca
     4801 ggcaaatctt ctttggtgaa tcagctctgc ggcgaggagc gccagctcgt ggacaatcgg
     4861 ccgggcacca cggtcgacag tgtcgatacc ttgatcgatc gcgacgggaa taggctcatt
     4921 ctgatcgaca ccgctggcat ccgtcgaaaa cgcagtgtca agaagcaggg cgtcgaagga
     4981 ctcagcgtgc ttcaggccat tcgttcgatc gagcgcagcc acgtggtcgt actgatgatc
     5041 gatggcgagg ccggggtggg cgaacaggac gcgaagattg cgggactggc tcatgaccgc
     5101 ggacgcgcgc tggtcctcgc tctgaacaag accgacctca tgagtcgaga cgagcaaaag
     5161 aaagccatgg accgcacgcg cgaggttctg gcattcgcac cttgggctcc gctcgtgagt
     5221 gtgagcgcac tcgaggggaa agggatcaat gcattgctcg gtcgagtggt ggatgccgtc
     5281 gaagagcacc gaaaacggat agggacggcc gagatgaatc gctttttcga ggaagtgctc
     5341 gacaaacacc cgccccccac gatgcaccat cggccggttc ggctctacta catcactcag
     5401 gcgagcgtcg cgccgcccac cttcgtggtc atgaccaact cgcccgacga cgtgcatttc
     5461 agctatcagc gatatgtggt caaccagatc cgacagcgct tcggcttcaa gggaaccccg
     5521 attcgcgtgc ggtatcgcgg aaaaaagaag aagtagttag gcgctcaaag tcggaagagt
     5581 tttccgcgcc cggcgggccg atggattgcc gagctgacca catgctgcca tgacgctccg
     5641 gcccttggtc tttcgctcac gcaccggaag cccttcggcg cgcagccagc cgatgaatcg
     5701 ctcgacctcg cgctcctcgg gcgcgcgcgt cagcggctgg gagccgggat tgtagggtat
     5761 gaggttcaag agaacgcgac cgagcggttc gcagaacgcc gcgattgcgc gcgcatcgga
     5821 ttgcgtgtcg ttgatgccag gaagcaggca atagttgacg cccaggacga agttgcggcg
     5881 gggccggtac ccggcgagtg tgttctgaag ctcagctagg tcggttcggc gattgattgg
     5941 catcagctcg ctccggagcg aatcgttcgg ggcgttcaag ctgattgaaa gattgagccg
     6001 cttgaggcct tgttggtcga gcatccgaat tccctcgaca tggcctacgg tgcagacggt
     6061 caggcgttct tgcccaatcg cgagaccggc ctcgtcgttc agcacccgca acgcgtggaa
     6121 tacgttgtgc gcgttgtcga gggcctcccc catgcccatg aatacgatat tgcgaatcgg
     6181 ccatcgaagc cggtgtcggg cgaggagcag ctgagcgatg atctcgtcgg tacggagatg
     6241 gcgaagcaaa cccatgcgtc cggtttcgca gaaggtgcat cccatcttgc aacccacctg
     6301 gctcgaaaca cacagggtgt acctgccacg ccccatcggg atgcaaacgg actc
//
LOCUS       DASRZF010000257         3917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4657595, whole genome shotgun sequence.
ACCESSION   DASRZF010000257 DASRZF010000000
VERSION     DASRZF010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4657595"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1515
                     /locus_tag="VFG22_11430"
     CDS             <1..1515
                     /locus_tag="VFG22_11430"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET6416898.1"
                     /translation="LNTTNKRVAAAAACLTLLSYIATAVISASEGMHYAHHLWDGLNV
                     FWATIGVLGGFALLGIIGIGESAVVALVIFLFHIAVLSVLVVASAIVVARDPSVLIEN
                     WRLPSVASVPHALFFGFAAAMLGISGFESSANFIEEQKEGVFPKTLRNMWIVVSIFNP
                     LISFLSLGLLPLSYIREVPPDLLAQMGEVAMGRVLGLLVSVDAILVLSGAVLTSFVGV
                     TGLARRMALDRVLPPFLLRENKWRGTNHWILIGFFGLCVSILLITHAEISSLAGVYTL
                     AFLSVMALFAVGNMLLKVKRARLPRAVRASWPTVVVALIAVLLGLLGNALLDPINIEI
                     FEAYFLVTAGAVALMFLRREILKLVLYVSRAVVQKLGRASKRVNDLLVEQIQRISESA
                     VVYFTRGDNIITLNRAVLYVLANEQTSRLKVIHVYQDEADIPPKLADQLKMIDHLYPE
                     LRVDFVAVKGKFTPQLIEAISQRLKIPKNYMFIGTPGDHFPHRIEDLGGVRLVL"
     gene            complement(1473..2732)
                     /locus_tag="VFG22_11435"
     CDS             complement(1473..2732)
                     /locus_tag="VFG22_11435"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6416899.1"
                     /translation="MRPKIPRNVWALSGTSFFMDISSEMILNVLPLFLANVLGVGTAV
                     IGLIEGVAESTASLLKVFAGGISDRMGARKPLAVGGYAVSALAKPLFYLASSWGVVAG
                     ARWADRVGKGVRTAPRDALIADSISPSGRGLAFGLHRAADTGGAVLGLLIAIIVVTQM
                     QSSTSELGRDTFRTLVLVSLIPAVLAVMTVAFGVKEVRKKATADRPRIGFRGLGRRFA
                     VFLGIVALFDLGNFSDAFLILRAQERGLGVSAVLWTLLGFNLVYALISTPAGALSDRI
                     GRRRVITAGWIAYAVIYLGFALAGTGTQIVLLYLSYGAYYGLATGTAKAMVADLVPVE
                     LRGTAYGSYHALLGVIDLPASLIAGVLWQGVGSWPGFGPAAPFYFGSATALAAALLFT
                     FFFRDEPKGSRDPDQSTSRTPPKSSIR"
     gene            complement(2798..>3917)
                     /locus_tag="VFG22_11440"
     CDS             complement(2798..>3917)
                     /locus_tag="VFG22_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416900.1"
                     /translation="SPSIAGAEPEKWHAGVLGGGEFNFAPNLNNQLSGHGWAGFDSVG
                     EGIVGGGNLRLFYNTTKAHVGIERLTFANGKLAFFAFLEGEAVISQLLNDYYKQGLRL
                     SEYSIKASYALLHTKLQWYPGKHQTIEILAPVRYWWFGDRSSTSPDYALPPNTWVFEP
                     RIGYNFWQIDVPGEEWESHRVFPRIKGFAVGIDGGLDVRSDAQPWGIADGRNNPGKVI
                     YSVSQWLKAGWQLGHFVRLQLDQWGNYGWRQDDITRQRIGGASPYVIPVPGLPWPALI
                     SERLFAGQLGLHLKAKESSQHEFGLLVAGGAFNDVYRVGALNTYGGAGGLTVFGDLRF
                     GPTGRYQVNIRGSWGFPAVWWENPPYIAVLAALGVRIF"
BASE COUNT          745 a         1231 c         1197 g          744 t
ORIGIN      
        1 ctcaacacga cgaacaagcg cgtcgccgct gccgccgcct gcctgacact gctgtcgtac
       61 atcgcgaccg ccgtcatcag cgcgagcgag gggatgcact acgcacatca cctctgggac
      121 gggctgaatg tcttttgggc gacgatcggg gtgctcgggg gctttgccct gctcgggatc
      181 atagggatcg gggaatcggc ggtggtcgcg ctcgtgatct tcctctttca catcgccgtt
      241 ctctcggttc tcgtggtcgc cagtgccatc gtcgtggccc gcgacccctc ggtcctgatc
      301 gagaactggc gccttccatc cgttgcgagc gttcctcatg cgctcttttt tgggttcgcc
      361 gctgcaatgc tcgggatcag cggtttcgag agctccgcaa acttcatcga ggagcaaaag
      421 gaaggcgtgt tcccgaagac gctccgaaac atgtggatcg tggtctcgat cttcaatccg
      481 ctgatcagct ttctttccct cggattgctt cccctgtctt acattcgaga agtgccaccc
      541 gacctcctcg cccaaatggg agaggtcgcg atggggcgag tgctggggct gctcgtcagt
      601 gtcgacgcga ttctggtcct ctcgggcgcc gtgctcacga gcttcgttgg ggtaaccgga
      661 ctcgcgcggc gaatggcgct cgaccgcgtt ctgccgccat ttctgctgcg agagaacaaa
      721 tggcgcggca ccaatcattg gatcttgatc ggctttttcg gcctctgtgt ctcgattctt
      781 ctcatcactc atgccgagat ctcgtcgctt gctggggtct atacgctcgc gttcctgagc
      841 gtgatggcgc tcttcgccgt tggaaacatg ctcctcaagg tgaagcgcgc ccgacttccg
      901 cgtgcggttc gcgcaagctg gccgaccgtc gtggtcgcgc tgatcgcggt cttgctcggg
      961 cttctgggca atgctctgct cgatccgatc aacatcgaga tcttcgaagc gtatttcttg
     1021 gtgaccgccg gcgccgtggc tctcatgttt ctacggcgcg agatcttgaa actcgtgctt
     1081 tacgtctcgc gagcggtggt gcagaagctc ggccgagcca gcaagcgggt caatgacctc
     1141 ctcgtcgaac agatccaacg gatcagcgaa agcgcagtgg tctacttcac ccgcggcgac
     1201 aacatcatca ctctcaacag ggccgtgctc tacgtgctcg cgaacgagca aacttcgcgc
     1261 ctcaaggtga tacacgtcta tcaagacgag gcagacatcc cgccgaagct cgccgaccag
     1321 ctcaagatga tcgaccattt gtatcccgag cttcgcgtcg atttcgtcgc ggtgaagggg
     1381 aaattcactc cgcaactcat cgaagccatt tcccagcgcc tgaagattcc gaagaactac
     1441 atgttcatcg ggacgcccgg cgatcacttt cctcaccgaa tcgaggattt gggcggggta
     1501 cggctcgtgc tctgatccgg atctctcgaa cctttcggct cgtcgcgaaa gaagaaggtg
     1561 aagaggagcg cagccgcgag cgcggtggct gatccaaagt agaaaggtgc ggcgggtcca
     1621 aacccgggcc aggagccgac cccctgccag agcacgccag caatgagcga ggcgggaaga
     1681 tcgatgactc cgagcagtgc gtggtagcta ccgtaagcgg taccgcgaag ctcgactgga
     1741 accaagtctg ccaccatggc tttggcggtt cccgttgcaa ggccatagta cgcgccgtag
     1801 ctgaggtaca agaggacaat ctgcgtgccg gtcccggcca gagcgaaccc gagatagatc
     1861 acggcatacg cgatccaccc cgccgtgatg acgcgccgcc gtccaatccg atccgagagc
     1921 gcgccggcgg gtgtagaaat cagcgcgtag acgaggttga acccgagcag cgtccagagc
     1981 accgcgctta cgccgaggcc gcgttcttgg gctcgaagga tcaagaaggc atccgaaaag
     2041 ttaccgaggt cgaaaagtgc gacgatcccg aggaagacag cgaaccggcg accgaggccg
     2101 cgaaaaccga tgcgtggtcg atcggccgtc gccttcttgc gaacctcttt gacaccgaac
     2161 gccactgtca tcacagcaag caccgcgggg atgaggctga ctaagacgag agtccgaaag
     2221 gtgtccctcc ccaattccga cgtgctgctt tgcatctgcg tgaccacgat gatcgcgatg
     2281 agcagcccga gcaccgcgcc ccccgtgtcc gcggcgcggt gcaggccgaa cgccaggccg
     2341 cggcccgacg gagagatcga gtctgcgatg agcgcgtccc ttggcgccgt tcgcacgcct
     2401 ttgccgacgc ggtctgccca tcgtgcgcca gccaccacgc cccagctgct ggcgaggtag
     2461 aagaggggct tcgccagagc ggacacggcg tagcccccta ctgccagcgg ctttctggcg
     2521 cccatccgat cggagatgcc gccggcgaac accttgagca ggctcgcggt cgactcggca
     2581 acaccttcga tgagcccgat caccgccgtt cccacgccga gcacattggc gaggaacagc
     2641 gggagcacgt tcaggatcat ctcgctcgag atgtccatga agaagctcgt gcccgaaagc
     2701 gcccagacgt tacgcgggat cttcggacgc attggggacg ctcttagcgc gctattggag
     2761 gatgaccaag cgggtcgccc tacccgctca atcttcgtta gaagatgcgg acaccaagcg
     2821 ccgcgagcac ggcgatgtac ggagggttct cccaccagac ggcaggaaag ccccagctac
     2881 cgcggatgtt gacctgatag cggccggtag gaccgaaacg caagtcgccg aacaccgtga
     2941 ggccgcccgc gccaccgtag gtgttgagcg cgcccacccg atagacgtcg ttgaatgcgc
     3001 caccggccac cagcagaccg aactcgtgct gcgagctttc cttggccttt agatgaaggc
     3061 cgagctggcc ggcaaagagc cgttcgctga tcagtgccgg ccaggggaga ccgggtaccg
     3121 gaatcacgta cgggctggcg ccaccgatgc gctggcgcgt gatgtcgtct tgtctccagc
     3181 cgtagttgcc ccattggtcg agctgaagcc ggacgaagtg tccgagttgc cagccggcct
     3241 tgagccattg cgacacgctg tagatgacct ttcccggatt gttgcgtccg tcggcaatac
     3301 cccagggctg agcgtcggaa cgcacgtcga gcccgccatc aatccccacg gcgaagccct
     3361 tgattcgcgg aaaaacgcgg tggctctccc actcctcgcc gggaacgtcg atctgccaga
     3421 aattgtatcc gatgcgtggc tcgaacaccc aggtgttggg cgggagtgcg tagtcgggcg
     3481 aggtcgagct tcgatcgccg aaccaccagt accgcacggg cgcgagaatc tcgatcgttt
     3541 ggtgcttgcc cggataccac tgcaacttgg tgtggagcag cgcatagctc gccttgatcg
     3601 agtactcgct gagccgcagg ccctgcttgt aatagtcgtt gagcagctgc gaaatcacgg
     3661 cctcgccttc gaggaaggcg aagaacgcca gcttgccgtt cgcaaatgtg agcctctcga
     3721 tcccgacatg cgccttggtc gtgttataaa acagtcgaag gttgccgccg ccgacgatgc
     3781 cctccccaac actgtcgaag ccggcccagc cgtgcccgct gagctggttg ttgaggtttg
     3841 gcgcgaagtt gaactcaccg cctccaagca cgccggcatg ccatttttcg ggttccgccc
     3901 cggcgatgga gggggag
//
LOCUS       DASRZF010000258         2943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1913884, whole genome shotgun sequence.
ACCESSION   DASRZF010000258 DASRZF010000000
VERSION     DASRZF010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1913884"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VFG22_11445"
     CDS             complement(<1..206)
                     /locus_tag="VFG22_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416901.1"
                     /translation="MIPIKLKPRILKTARENGRVTTGELSAPLEPTQKLSLGEQRYTR
                     YAGKSRKNRWIDGVRPGDGDDPLE"
     gene            complement(218..1045)
                     /locus_tag="VFG22_11450"
     CDS             complement(218..1045)
                     /locus_tag="VFG22_11450"
                     /inference="COORDINATES: protein motif:HMM:NF021451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2163 domain-containing protein"
                     /protein_id="HET6416902.1"
                     /translation="MADYDTLETSVEGSRPIEVYRITLGSSEYLYTTSEDDLTIDGDD
                     YEAIPISRNNIVVGSDQQRRAIQITVPATNDFARQYLSSLPGEVASVSIFRYQRDESP
                     AFDTTVLLFTGTVQSVQFSEDGTRAQINVRSLEGALSRNVPRFTFLGQCNHFLFDRSC
                     GVDPSAFNHVGVVTVVSGYDITISGLAASGFDFVGGYARPVSENDFRLVLSQSTDTLT
                     LILPFQNDQAGNNVQVFAGCDHVLTGDCALTFDNVANFGGFAFVPTWNVFQSGIKLG"
     gene            complement(1058..2905)
                     /locus_tag="VFG22_11455"
     CDS             complement(1058..2905)
                     /locus_tag="VFG22_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416903.1"
                     /translation="MSGVEYAGTLGSATLLPPSAPSLDFYPGTGENNSGIPPVFNQWV
                     QDAPNRAPAVSSQIPAFTAGGTITADTEGTPASPGAATEYPASSRFDSASFNAQDWFE
                     AVHILPRTKIDFGNIITQVDETYEIYSAFRFATTVTESAITNNASPGVTLPDNTPTVD
                     VPPLTSILDPTTSGQTTLGLGTIVLAKVRASASGVPTFDTDIIFEFDPPGNDVTLLVT
                     GSRIVLLPFEYEVGAVETLSFLTDIITSVSGAEQRISARKQPRQGFQVEYALVEEERQ
                     AMHTILFDWQAGIFGFPVWHERVTLTAATSVGATSYPVSGADDVDFRVGGSAVVISDY
                     LTYDVIEVSAQTDTLVSATSTAVNAYAAGTAIMPLRIARIPFRIDAERLLSQENFKIS
                     FEVTDNDTGALAGDTTPGFWSTYNSRVLFDDCNVIDRAGSPTKLSKRVYVVDNETGLV
                     DQSFSWAQNKRESQKGFVARDRAEILALRKLFIALRGKAVSLYLPTFINELTPNATLA
                     SGTDTMDIASIGYTRFAQDRLPMALFRITFTDGTQLVREVASSAIVTTATERLTLNTT
                     WPSTKLVSEVSRIEWYELVRFDSDVMRLTYPRIGLATCTMPVIRVFDDN"
BASE COUNT          554 a          901 c          910 g          578 t
ORIGIN      
        1 tcctcaagag gatcatcccc atccccgggc cgcacgccgt cgatccagcg gttcttacgc
       61 gacttgcccg cgtagcgcgt gtatcgctgc tcgccaagcg agagcttctg tgtcggctcc
      121 agaggagcgc ttaactcgcc cgtcgtgacg cgcccattct cgcgcgcggt cttcaggatt
      181 ctcggcttta gcttgatagg aatcatcgaa tcgtcaccta tccgagcttg atgccgctct
      241 ggaacacgtt ccacgtcggg acgaaggcga agccgccgaa gtttgcgacg ttgtcgaacg
      301 tgagtgcgca gtcgcccgtg agcacatgat cgcagccagc gaacacctgt acgttgttcc
      361 ccgcctggtc gttctggaac ggcaggatca aagtcaacgt atccgtggat tgcgagagca
      421 cgagccggaa gtcattctcg ctcactggcc gcgcgtagcc gcccacgaag tcgaagcccg
      481 acgccgcaag ccctgaaatc gtgatgtcgt atccactcac gaccgtgacg acgcccacgt
      541 ggttgaacgc cgatgggtca accccgcatg atctgtcaaa gagaaagtga ttgcactgac
      601 caaggaacgt gaagcgcggc acgttgcgtg acagcgcccc ctcgagcgag cgcacgttga
      661 tctgcgcccg tgtgccgtct tccgagaact ggaccgattg aacggtgcca gtgaagagca
      721 gaaccgtcgt atcgaaagcc ggcgactcat cgcgctgata tcgaaagatg ctgacgctcg
      781 cgacctcgcc cgggagtgag ctgaggtact gacgcgcaaa atcattcgtc gccggcaccg
      841 tgatttggat cgcgcgtcgc tgctggtcag agcctaccac gatgttattg cgcgagattg
      901 ggatcgcctc gtagtcgtcc ccgtcgatgg tgagatcgtc ctcggacgtg gtgtacaggt
      961 actcgctcga ccccaaggtg atgcggtaga cctcgatcgg gcgcgagccc tcgacgctgg
     1021 tttccagggt gtcgtaatcg gccatgcgtg ccttccccta gttgtcgtca aagacccgga
     1081 tgaccggcat cgtgcaggtt gcgagcccga tacgcggata ggtcagtcgc atcacgtcgc
     1141 tgtcgaagcg caccagctca taccactcga tccgcgagac ctcggagacg agcttggtgc
     1201 tcggccatgt cgtgttcaaa gtgagacgct cggtcgccgt ggtcacgatc gcgctcgatg
     1261 ccacctcccg tacgagctgt gtgccgtccg tgaacgtgat gcggaagagc gccatcggaa
     1321 gacgatcctg cgcgaaccgt gtgtacccga tggacgcgat gtccatggtg tcggtgcccg
     1381 aggcgagcgt cgcattcggc gtcagctcgt tgatgaacgt cggcaggtag agcgatacag
     1441 ccttgccacg caacgcgatg aacagctttc gaagcgcaag gatctccgcg cggtcgcgcg
     1501 ccacgaaacc cttttgcgat tcgcgcttgt tctgcgccca ggaaaacgac tggtccacaa
     1561 gccccgtctc gttgtccacg acatagacgc gcttactgag cttcgtgggc gatcccgcgc
     1621 gatcgatgac gttgcagtcg tcgaagagca cgcggctgtt gtaagtcgac cagaagcctg
     1681 gcgtagtatc tcccgcgaga gccccggtat cgttgtccgt cacctcgaag gaaatcttga
     1741 agttctcctg cgatagcaag cgctccgcgt cgatgcgaaa cgggatgcgc gcgatcctaa
     1801 gtggcatgat cgctgttccc gccgcgtaag cgttcaccgc tgtactggtc gcggagacca
     1861 gagtatccgt ctgcgccgag acctcgatca cgtcataggt cagatagtcg gagatcacga
     1921 ccgccgagcc gcccactcgg aagtctacgt catcagcccc cgacaccggg tagctcgtgg
     1981 caccgaccga tgttgctgcc gtgagtgtca cgcgctcgtg ccagacgggg aagccaaaga
     2041 tcccagcttg ccagtcgaag agaatcgtat gcatcgcctg gcgctcttct tcgaccagcg
     2101 cgtactcgac ctgaaagccc tgccgcggtt gcttgcgtgc cgagatcctc tgctctgcac
     2161 cgctcacgct cgtgatgatg tccgtgagaa acgagagcgt ctcaacagcg ccaacctcgt
     2221 actcgaatgg caggagcacg atgcgtgagc cggtcacgag gagcgtcacg tcgtttccgg
     2281 gcgggtcgaa ttcgaagatg atgtcggtat cgaaggtcgg caccccgctc gccgaagcgc
     2341 gcaccttggc aagcacgatg gttccgagcc cgagcgtcgt ctgccccgag gtcgtggggt
     2401 ccaggatact cgtgagcggc gggacatcga cggtcggcgt gttgtccggc agcgtgacgc
     2461 cgggagacgc attgttcgtg atcgcgcttt cggtcactgt cgtcgcgaac cggaacgcgc
     2521 tgtagatttc gtaggtctca tccacctgcg tgatgatgtt cccgaaatcg atcttggtcc
     2581 tcggcaggat gtggaccgcc tcgaaccagt cctgcgcgtt gaagctcgcc gagtcgaaac
     2641 gggagcttgc cggatattcc gtggcggccc caggagaggc cggcgtgccc tctgtgtctg
     2701 cagtgatggt gccgccggcg gtgaatgcag ggatctgact cgaaaccgct ggagcccgat
     2761 tcggcgcatc ctgcacccac tgattgaaga caggcgggat accggaattg ttctcgccgg
     2821 tccccggata gaagtcgagc gacggcgccg acggcggcag cagcgtcgcg gatcctagag
     2881 tgcctgcgta ttcgactcca gacatcggct aggtcgtgac cttcttgtag gcgatgccct
     2941 ggt
//
LOCUS       DASRZF010000259         8747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3289624, whole genome shotgun sequence.
ACCESSION   DASRZF010000259 DASRZF010000000
VERSION     DASRZF010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 8747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8747
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3289624"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..771
                     /locus_tag="VFG22_11460"
     CDS             118..771
                     /locus_tag="VFG22_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416904.1"
                     /translation="MRLVLIGMLAGALGLVGCGDDATSDVGTGGTAGSGGTGGSEPLE
                     ACSEGPLAETGQTGTGALACVATLPFDLSLRFNATPREPLQAGANTFDLQIEVAIDAD
                     TVDTVIGLAPGADVTSVEGTIDATVGDSDPTPVVVNDEGVPCLLTFEESAPAVIVTTV
                     SEGTWNLDDGGTLELTLGAVTQAVTALNIPVVLTTEGDEPSCEFVGNLPSVQFTLPQ"
     gene            1023..2435
                     /gene="clcA"
                     /locus_tag="VFG22_11465"
     CDS             1023..2435
                     /gene="clcA"
                     /locus_tag="VFG22_11465"
                     /inference="COORDINATES: protein motif:HMM:NF003640.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HET6416905.1"
                     /translation="MKLPSPISVIVSRAASSTRLARRFLEAQRKSGPLLLAAAAVGTL
                     TGGLASLFHLVLDWIAELRISHSPPSDPWATWVVVPAGMAVCLLVAVYLVRKLAPEAG
                     GSGVQIVEGALDGVLPMRWRRVIPVKFIGGSLALGSGLLMGREGPSIQMGAAIGQALV
                     DLFRLPKTMIHGLIAAGAGAGLTAAFNAPFAGILFVIEEMRPQFHYSVLSVQAVVIAC
                     AISDVVMRLLIGDVAAIAMPVLDAPELSMLWLFVVFGVAFGFVGYAFERFLLRSTDWC
                     SRLSARGLTLGIVLVGLSIGWIGALDFRFVGGGENVLEGFLTMSTPISVLGAVFAMRF
                     FATIASYSTGAPGGLFAPMLALGTIGGVAYGELIVALDLGAGLIPAHFAVAGMGAFFA
                     AVVRAPLTGIILTAEITGNFTQILPLMLTCMAATITAHGLGTRPIYTALLDRDLDRIH
                     RTAGEPQVALPEAEEIPVEV"
     gene            2571..3227
                     /locus_tag="VFG22_11470"
     CDS             2571..3227
                     /locus_tag="VFG22_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416906.1"
                     /translation="MKTKTRFAVLCGCVLALVACKTTDTKFSQSYRNPGYEETSFKKV
                     MVIGVAQDQKARQAFEDALVSAIAKEGGTAQASIAVLPNEEQISEDQLHAAIDAGGFD
                     AVLITRVLSVDKSQQYTPPKKYNNPQTRYYPGSLGWGHGYGGYYGFYGTTFAEVHEPG
                     YFETSTTLRLETNLYSVATNELVWTGQSATIDPQSIDDARSSMTQAVAKKLKEERFIP
                     "
     gene            complement(3263..3682)
                     /locus_tag="VFG22_11475"
     CDS             complement(3263..3682)
                     /locus_tag="VFG22_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET6416907.1"
                     /translation="MWDFISEARIPIRLAANTATGFPVVLSLWFLPENEQLLGAVHRD
                     SRIAKRLEADPRCAFEIASNEPPYRGVRGQAIATLEPEGAGILLERLLRRYLGSTESS
                     LGRFLLARAEEELVVRLQPSWIGSWDYSRRMDDALEG"
     gene            3944..5428
                     /locus_tag="VFG22_11480"
     CDS             3944..5428
                     /locus_tag="VFG22_11480"
                     /inference="COORDINATES: protein motif:HMM:NF022518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HET6416908.1"
                     /translation="MRLVTAPERHSNQGRFGLAELIDFEVQLASDRDRPREELIARDR
                     AIALRWQNHPKSATELVTRWLASLPRSSAGSTAGERVERAHVIANWLVFVVFALIGVT
                     IALAVLQFTGDHPINVFVVLGVFVFLQWVNLLGTFAAFVWSRSSPGFLAQFPLSALVR
                     KLIVKIAGADDAGRFLARRSLYGGVERWVLFRAMQLGAVAFNVGAISTFIAAVSFTDL
                     AFAWSTTLQVGAEGLQRFCEGLAAPWRLWLPDAVPTLDLVGATQYFRIDSAYVNAPAG
                     ARVRDAALTGGWWPFLLMSLLVYGLLPRLALAVWGTVGMHRAFHKLSFDTPEETEVIA
                     RLTNPRVRRVQGNDPGNVAPLGEGHPPLDRPQQFNRDEPAIAVRWRDAEISDDAFLRR
                     LHERFGARVEAPIASAGGHDHREDEALLRRIERSEQPVLLLAEPWNAPDRAFKRLIQS
                     LRAHGHSSRKIYVLLTTGGSDEQRGVWAGYLAELGDPYIALDAL"
     gene            5425..6879
                     /locus_tag="VFG22_11485"
     CDS             5425..6879
                     /locus_tag="VFG22_11485"
                     /inference="COORDINATES: protein motif:HMM:NF023407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3482 domain-containing protein"
                     /protein_id="HET6416909.1"
                     /translation="MSEGASEIPRFAVVGRVNKGKSSVIASLIENDAVKISPRPGTTT
                     ECVRYDVEVDGRVLFSVIDTPGFEDAPRALHWIGQQEVTAADRVQRVRDFVDAFEGTE
                     DFVEERRLLRPILDGSAILYVVNGDEPYRKNYETEMEILRYTGQPAMALINRSGSGEH
                     IEDWQRALNQYFKLVRLFDAHAVTWEERRKLLEAFATLQPSWERSIIEAVDALRVQEE
                     HRRYQVANIISDMMLDALSLHLSVVLPDEHAFPHEKTRLEREFHDKLRVIERKAHRKI
                     AEVYLHRLDHWSPETEIELESHEDLFAKETWDVMGLSPKALLTLTTLTGAAAGGAVDA
                     AVGFASVFAGTVLGGLGGLGVGLYELTRRFASASNITEQAMSAIRNPKDGYRVRVGPH
                     PSLNFPFVLLARALDHFERIQAWAHAKKELPEPTENGPKILDQLDTSTRRRLSKTFSK
                     IQKKYRDVPKETRRELRDLVHQIVVKNAKSLRVG"
     gene            complement(6977..7597)
                     /locus_tag="VFG22_11490"
     CDS             complement(6977..7597)
                     /locus_tag="VFG22_11490"
                     /inference="COORDINATES: protein motif:HMM:NF025850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET6416910.1"
                     /translation="MPHLKLTYFDIHGGRAEPARLAMYIGGVAFEDNRISFQEFDANR
                     SKYPFERVPLLEIDGVALSQCNSINRYAAKLAGLYPSDPLQAAFCDEAMDAVEDIVSQ
                     ITVTMRIKDEAEKKAARAALADGAIKIYLEQLQTMLESRGGQYFADGRLTVADLKVMV
                     CVRYLRSGIIDHVPRDLPDRLAPRLVEHLGRIKNHPKVREYYARFS"
     gene            complement(7637..8473)
                     /locus_tag="VFG22_11495"
     CDS             complement(7637..8473)
                     /locus_tag="VFG22_11495"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HET6416911.1"
                     /translation="MRIHPSTVLVILPLMLACSTTMTGRRQLTLIDDQKMDELGVAAF
                     NDLKKETKVSKDSAAAAYVRCVADALLTVVPKGGPAPKDGRWEVLVFDDPTPNAFALP
                     GGKIGVHTGMLTVATTAGQLAAVIGHEIGHVLLRHGNERMSQGMVAQSVVEAGSVAID
                     QMQPAYRDVVVGGLGVGAQYGVLLPFSRKHESEADIVGQRFMAEAGFNPAEAIKLWEK
                     MAELSQGQEPAQWMSTHPANESRIESLQANLPKTRDLYRKARSAGKSPKCVPPAASSS
                     RS"
     gene            complement(8489..>8747)
                     /locus_tag="VFG22_11500"
     CDS             complement(8489..>8747)
                     /locus_tag="VFG22_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416912.1"
                     /translation="PEQNEVLLTGTTTNDNFPPCIVELEDAFRLLLDDPDVSLADNDT
                     VQVTWDHTEADVSIVAGDCEGEWFVPLPCAGEATFTLTQEF"
BASE COUNT         1638 a         2663 c         2725 g         1721 t
ORIGIN      
        1 gccttcctgg acgactccag atacacgggt cgggcgcgtg ttgggcgcaa tgggctcgtc
       61 tgtgccaagc ttcgcactga ccgggcgttt ctaggagaat agaaggatat gaaattcatg
      121 agacttgttt tgattggcat gcttgcgggc gcgctcggtt tagttggttg cggcgatgac
      181 gcgacttcgg atgttggcac cggtggtacc gcgggctctg gcggcactgg cggcagtgag
      241 ccactcgagg cgtgcagcga gggcccccta gctgaaactg gtcaaaccgg aacgggtgct
      301 ctggcttgcg ttgcaacgct gccatttgac ctctccttgc gcttcaacgc gaccccaagg
      361 gagccgcttc aggcgggcgc caacacattc gacctacaga tcgaagtcgc catcgacgcc
      421 gacacggtgg acacagtcat cggtttggcc ccgggagctg acgtgactag cgtcgaaggt
      481 acgattgacg ccactgtggg cgacagcgac ccgacaccgg tcgtcgtcaa cgacgaaggc
      541 gttccttgtc tgctgacgtt cgaagagagt gcgcctgcag tgatcgtgac cacggtgagc
      601 gaaggaacat ggaacctcga tgatggtggg accttggagt tgacgctggg cgccgtcaca
      661 caggcggtta cagcgctcaa cattccggtc gtcttgacga ccgagggaga tgagccgtcc
      721 tgtgaattcg tcggtaacct cccctctgtc cagttcacat tgcctcaata gcagtaccct
      781 ctgtcgcaac gtcgttgagc cccgtcggtc ttaccgtcgg ggctcaacgc gttctggagc
      841 cgccgaactg gcaaattcgt ccggaggcta gcttggcggg cagcgttggg tgagtctccc
      901 cggggccctc gttcgcggga acctcgccgt gtttgctgag acacgcattg cgccaccggc
      961 gctcagcatc tagatcgcgg cgtggccgac ggtcctcgca agcttgcaag acgaacccgg
     1021 caatgaagct accatcccca atctccgtca tcgtgtcccg tgccgcttcg tcgactcggc
     1081 ttgcccggag gttcctggaa gcccagcgca agagcggtcc gctccttttg gccgcggccg
     1141 ccgtgggcac tctcacggga gggctggcaa gcctctttca tctggtcttg gactggatcg
     1201 cagagctacg catctcgcac agccccccca gcgatccgtg ggccacctgg gtagtcgtgc
     1261 ccgccggcat ggcggtctgc ctgctcgtcg cggtgtacct agtgagaaag cttgccccgg
     1321 aagccggggg cagcggcgtg cagatcgtcg aaggagcgct cgacggtgtt ctgccaatgc
     1381 gttggcgccg tgtcatcccc gtcaagttca tcgggggcag tctagcgctc ggctcaggtt
     1441 tgttgatggg acgcgaaggg ccctccattc aaatgggagc cgcaatcggt caggcgttgg
     1501 tggatctgtt tcgactcccg aagaccatga ttcacggcct cattgcagcc ggcgcgggcg
     1561 cgggactcac ggctgcattc aacgcgccct tcgccggaat tctgttcgtg atcgaagaga
     1621 tgcggcccca attccattac agcgtcctgt ccgtccaggc cgtcgtcatc gcttgtgcga
     1681 tctccgatgt agtgatgcgt cttctcatcg gcgacgttgc ggcgatcgcg atgcccgtcc
     1741 tcgatgcgcc cgagctcagc atgctgtggc tgttcgtggt tttcggggtg gcattcgggt
     1801 tcgtcggata tgccttcgag cggttccttc tccgctcgac cgactggtgc tcgagattgt
     1861 ccgcacgggg tttgactctt ggaatcgtgc tggtgggctt gtcgatcggg tggatcggcg
     1921 cgctcgactt tcgcttcgtc ggcggtggcg agaacgtgct cgagggcttt ctgacgatgt
     1981 cgacgccgat ctcagtgctc ggcgccgtgt tcgcgatgcg cttcttcgcg accatcgcga
     2041 gctactccac cggtgcgccc ggcggtctat ttgctccgat gctcgcgctc ggaaccatcg
     2101 gaggcgtggc ctacggagag ctcatcgtcg ccctcgatct aggcgcggga ctgattcccg
     2161 cacatttcgc ggtcgcgggg atgggcgcct tttttgccgc ggtggtgcgc gcgcccttga
     2221 ccggcatcat tctcacggca gagattacgg gaaatttcac gcagatcctt cccctgatgt
     2281 tgacctgcat ggcggcgacc atcacggcac acggcctggg cacccgccca atctacacgg
     2341 cgttgttgga ccgcgatctc gaccgcatcc atcggaccgc gggggagccg caagtcgccc
     2401 tgcccgaagc agaagaaatc ccggtggaag tgtgacgcgt tccccggcgc acgcgggcgg
     2461 cgaggacgag ccacccaagg acggaccgcc acgcgcatcc gcctcaccct cgtatcggat
     2521 cgtccgcgag ctggacatgg aatgagcgtc gactagatag aggatagatc atgaagacaa
     2581 agacccgctt cgccgtgctg tgcggctgtg tgctcgcgct cgtcgcgtgc aaaacgaccg
     2641 acaccaagtt ctctcaaagc taccggaatc cgggctacga agaaacgtcc ttcaagaaag
     2701 tgatggtgat cggcgtcgcc caagaccaga aagcccggca ggccttcgag gacgcgctcg
     2761 tctccgcgat cgccaaagag ggtgggacgg cccaggcgag tatcgccgtg ctaccgaacg
     2821 aagagcagat ctcggaggac cagctgcacg ccgcgatcga cgctggcggt ttcgatgcgg
     2881 ttctcatcac acgtgtcttg agcgtcgaca agtcgcagca atacacgccg ccgaagaagt
     2941 acaacaaccc acagaccagg tactacccag ggtcgctcgg gtggggacac gggtatggcg
     3001 gttactacgg gttttacggg accacttttg ctgaggttca cgagccgggc tatttcgaaa
     3061 cgagcacgac gctgaggcta gaaaccaact tgtactcggt cgcgaccaac gagctcgtct
     3121 ggacggggca gtccgcaacc atcgaccccc agtccatcga cgacgcccga agctcgatga
     3181 cccaagcggt cgccaagaag ctcaaagaag aacggtttat cccgtagatc gcgatcgagc
     3241 ccgggggaac cggactgcgg cttcatccct cgagcgcgtc gtccatgcgt cgactgtagt
     3301 cccaggatcc tatccaagat ggctgcagac ggacgacgag ctcctcctcg gcacgagcga
     3361 gtagaaagcg tccgaggctc gattcggtcg agccgagata tcggcggagg agacgctcca
     3421 gcagaatacc tgctccctcc ggctcgaggg tcgcgatggc ctgaccccgc actccacgat
     3481 agggcggctc gttcgaagcg atctcgaatg cgcagcgggg atccgcctcg aggcgtttcg
     3541 cgatccgaga gtcccggtga accgctccga ggagctgttc gttctcgggc aagaaccaaa
     3601 gggacaggac gactggaaat cccgtcgccg tatttgccgc gagccgaatg ggaatgcgcg
     3661 cctcagaaat gaaatcccac acctgggatt tggtccaagg acccgtgcgt cgagttgtcg
     3721 gcatgagaag tcgtccgtgt ttcagcgagg tcgaagaccg atggtcatgt atgcggcatg
     3781 gctcagagat aacacatcgc accaagttct gtcctgctca gaagagcgac cgagcgatct
     3841 tctcgcagcc gagattcgca tgaaccgatc cacgccgcag aaccaaacgg gcaatcgcat
     3901 tcatccggag gcgggttggc gtcgggctag gcttatagta ccaatgcgct tagtgaccgc
     3961 gcccgagcga cattcgaacc agggtcgctt cggcctcgcg gagctcatcg atttcgaggt
     4021 acagctcgcg tctgacagag atcgtccgcg cgaggagctc atcgcccgag accgcgcgat
     4081 tgcgttacgc tggcagaacc acccaaagag cgcaacggag ctcgtgacgc gttggttggc
     4141 atcgcttccg cgctcaagcg cgggttcgac ggctggcgaa cgcgtcgagc gggcacacgt
     4201 catcgcgaac tggctggtct ttgtggtgtt cgctctgatc ggagtcacca ttgcgctcgc
     4261 agtcctgcaa ttcacgggtg accacccgat caacgtcttc gtcgtgcttg gcgtgttcgt
     4321 gttccttcag tgggtcaact tgctcggaac gtttgctgcc ttcgtctggt cgcgttcctc
     4381 tcccggcttt ctcgcgcagt ttccattgag cgcactggtt cgaaagctga tcgtcaagat
     4441 tgctggcgcc gatgatgccg gacggtttct ggcgaggcgt tccctgtacg gcggagtaga
     4501 acgttgggtc ttgtttcggg ccatgcagct tggcgccgtt gcgttcaatg tcggcgcgat
     4561 ttcgaccttc atcgcagccg tttcgtttac cgatctagcc ttcgcttgga gcacgacact
     4621 gcaggtcgga gctgaggggc ttcagagatt ttgcgaaggc ttggctgcgc cgtggcgact
     4681 ctggttgccc gatgcggttc caaccctcga cttggtcgga gctacacagt atttccgcat
     4741 cgactcggcg tatgtgaacg caccggctgg tgcgcgcgtg cgagacgccg ccctcaccgg
     4801 ggggtggtgg ccttttctct tgatgtccct tctggtgtac ggactgctcc cgaggctcgc
     4861 gctcgcggtc tggggtaccg tgggtatgca tcgcgcgttc cataagctct cattcgacac
     4921 gcctgaggaa accgaggtca tcgcacgcct caccaacccc cgcgtgcgtc gcgtccaggg
     4981 gaacgacccc ggcaatgtgg cgccgctcgg cgaagggcac ccacctttgg accgaccgca
     5041 gcagttcaac cgagacgagc cggcgatcgc ggttcgctgg cgagacgccg agatctccga
     5101 cgacgcgttc ttgaggcgct tgcacgagcg cttcggcgcg cgggtcgaag cgccgatcgc
     5161 aagcgctggc ggccacgacc accgcgagga cgaagccctg ctcaggcgaa tcgagcgcag
     5221 cgagcagccg gtattgctgc tggccgagcc ctggaacgca cccgatcgcg cattcaaacg
     5281 tttgattcaa tctcttcgag cccacggtca ctcgagccga aagatttacg tcttgctgac
     5341 cacgggcggc agcgatgagc agcgtggggt ctgggccggg tacttggccg agctgggcga
     5401 cccgtacatc gcattggacg cgctatgagc gagggagctt ccgaaattcc gcgctttgcg
     5461 gtggtcggtc gagtcaacaa gggcaagtcg agcgtcatcg cgagcctcat tgagaacgac
     5521 gcggtcaaga tctcgccgag gccaggtacg accaccgaat gtgtccggta tgacgtggag
     5581 gtcgacggtc gagtcttgtt ctcggtcatc gatacaccgg gcttcgaaga cgcacctcgg
     5641 gcccttcatt ggatcggcca gcaggaggtc actgccgcgg atcgagtcca acgcgtgcgc
     5701 gacttcgtgg atgcgttcga gggcaccgaa gatttcgtgg aagagcggcg tctgcttcgg
     5761 cccattctgg atggctcggc gatcctctac gtggtgaacg gtgacgagcc ataccgaaag
     5821 aactatgaga cggaaatgga gatcttgcgc tacacggggc agccggccat ggcgctgatc
     5881 aaccgcagcg gctcgggcga gcacatcgaa gactggcagc gtgcactcaa tcaatatttc
     5941 aagctcgtga ggcttttcga cgcccatgcg gtgacctggg aagagcgccg caagcttctc
     6001 gaagcctttg cgacgctgca accttcgtgg gagcgctcca tcatcgaagc ggtcgacgcc
     6061 ttgcgggtgc aggaagagca ccggcgttat caagtggcca acatcatttc cgacatgatg
     6121 ctcgatgcgc tctcgttgca tttgagcgtc gtgctccccg atgaacacgc ttttccgcac
     6181 gaaaaaacac gcctcgagcg agagtttcac gacaagcttc gcgtgatcga aaggaaggcg
     6241 catcggaaga tcgccgaagt ctacttgcac cgcctcgacc actggtcgcc agaaacggag
     6301 atcgagctgg agtcgcacga agacctcttt gcgaaggaaa cctgggacgt catgggcctc
     6361 tcaccaaagg cgctcttgac actcacgacg ttgaccggag ctgcagcagg tggggcggta
     6421 gacgccgcgg tgggcttcgc gtcggtgttc gccgggaccg tgctcggggg gctcgggggg
     6481 cttggggtcg ggctttatga gctcacgcgc cgatttgcga gcgcgtccaa catcaccgag
     6541 caggcgatgt ccgccatacg caacccaaaa gacggctacc gagttcgcgt ggggccccac
     6601 ccttcactca atttcccttt cgtccttctg gcaagagcgc tcgaccactt cgaacgcatc
     6661 caggcctggg cgcacgccaa gaaggaactt ccggaaccga ccgagaacgg cccgaagatc
     6721 ctcgatcaac tcgacacatc cacgcgacgt cggctcagca aaacgttttc gaagatccag
     6781 aaaaagtatc gagacgtccc gaaagagacg cggcgtgagc tacgcgatct cgtccaccag
     6841 atcgtggtca agaacgcgaa atcgctgcgt gttggctagg gaaagagata gacgtaccgg
     6901 catcagaacg tctaccgagt tcgatggtcg agtcgccaca gggagcaagt ggaaccgaac
     6961 gactcgagat actagctcac gagaagcgtg cgtagtactc gcgaaccttg ggatggttct
     7021 tgatccgccc gaggtgctcg acgagcctcg gagccagcct atcgggtagg tcgcgaggaa
     7081 cgtggtcgat gatgccggag cggaggtagc gcacacagac catcaccttg aggtcggcga
     7141 cggtgaggcg cccgtcggcg aagtactggc cgccacgcga ctcgagcatc gtctgaagct
     7201 gctcgaggta aatcttgatc gcgccatccg ccaaggccgc ccgcgctgcc ttcttctcag
     7261 cttcgtcttt gatgcgcatg gtgacagtta tttgcgaaac gatgtcttcg acggcgtcca
     7321 tggcctcatc gcagaacgct gcttgcaacg ggtcactcgg gtagagaccc gcgagcttcg
     7381 cggcgtaacg attgatgctg ttgcactgac tgagtgcgac gccgtcgatt tccaagagcg
     7441 gcacccgctc gaacggatac ttcgaacggt tcgcgtcgaa ctcttgaaag ctgatgcggt
     7501 tgtcttcgaa ggcaacgcct ccgatgtaca tggcaagcct cgccggctcg gcgcgacctc
     7561 cgtgaatgtc gaaataggtg agcttgaggt ggggcatgac cgtactctaa ggccaaacgt
     7621 gcccgtgtcg caccaatcag ctgcggcttg aactagccgc tggcggaacg catttcgggc
     7681 tcttgcccgc cgaacgcgcc ttgcgataga gatcacgcgt tttcggcaga ttcgcttgga
     7741 gactctcgat tcggctttcg ttcgcggggt gggtggacat ccactgagcg ggctcctgac
     7801 cttggctcag ctcggccatc ttctcccaga gcttgatcgc ctccgcaggg ttgaacccgg
     7861 cttcggccat gaaccgctgc ccgacgatgt ccgcctcgct ttcgtgcttt cgactgaagg
     7921 gcaagagcac cccgtactga gcgccgacac ccagcccgcc gacgaccacg tcgcgatacg
     7981 cgggttgcat ctgatcgatc gccaccgagc cggcctcgac cacgctctgt gcgaccattc
     8041 cctgcgacat ccgctcgttt ccatgacgca gtaggacatg cccaatctcg tgtccgatga
     8101 cggccgcgag ctgtcccgca gtggttgcga ccgtcagcat gccggtgtgc accccgatct
     8161 tcccgcctgg aagggcaaat gcgttggggg tgggatcgtc gaagaccagc acctcccaac
     8221 gtccgtcctt gggcgcagga ccacccttgg gaacgacggt caagagggca tcggccacgc
     8281 agcgtacata cgccgcggcg gccgagtcct tcgagacctt cgtctccttt ttcagatcgt
     8341 tgaatgctgc gacgccgagc tcatccatct tctgatcgtc gatcaatgtg agctgccgtc
     8401 gacccgtcat ggtcgtcgag caggccaaca tcaacggcaa gatcaccaga acggtggatg
     8461 gatggatacg catcgagtcg tgccgtcgtc agaactcttg agtaagcgta aacgtcgcct
     8521 cacctgcgca gggcaagggc acgaaccact cgccttcgca atcacccgca acgatgctga
     8581 cgtctgcctc ggtgtgatcc caggtgacct gcaccgtgtc gttgtccgcg aggctcacgt
     8641 ccggatcgtc tagcaagagc cgaaatgcgt cctcgagctc gacgatgcag ggagggaaat
     8701 tgtcattggt ggtagtccca gtcagaagga cctcgttctg ctccggg
//
LOCUS       DASRZF010000260         8065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4660009, whole genome shotgun sequence.
ACCESSION   DASRZF010000260 DASRZF010000000
VERSION     DASRZF010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 8065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8065
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4660009"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..1192
                     /locus_tag="VFG22_11505"
     CDS             161..1192
                     /locus_tag="VFG22_11505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET6416913.1"
                     /translation="MALGCEGEAPAKSGASGPPPARVWVGEVRTGSVQAEWSFLGDVS
                     ALQHAQLAAGASGEVRRVLVRVGDRVKKGDLLVEIDPSLAAARVRAASASKEAGAAQL
                     ERAQRDAERLSTAGPDIAARAEIEQATSERKRAAAEGGRLKAAEAEARAELERHRITA
                     PFDGVVANRRVDPGDWVDPGVEAIELIDDRDVDVLASVPPEVASFLSVGDKANLRLRG
                     ETTPATIRGIVPALDSESRTVRLRLVPDEPPVWLLPGAAVDVIITVARSEEGALVVPR
                     DALVYGIAKTQIVKSVEGKAAPISVEVLASGREAVLIRAEELSAGDLVVTRGNERVFP
                     GQPLIVLED"
     gene            1197..4355
                     /locus_tag="VFG22_11510"
     CDS             1197..4355
                     /locus_tag="VFG22_11510"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET6416914.1"
                     /translation="MTHDQNHPPEEQPTGLSWLAIQRPITVIVGIVLVVLFGTLSVMD
                     LPIQLTPDIATPTITINTVWPGAAPVEVETEILEPQEEVLKNVQGLTRMESSASMDTG
                     SITLEFEVDTVIEEALVRVTNKLSQVSSYPEAVREPVVETANSAGPPLAVVAIRDPQK
                     GSVAAYRTWVQDEILPELDRIPGVAGIFFIGGQDTEVHVLFDAQALAARGLSVQGVAD
                     RVRSELRDLSGGDVTLGKRRYLVRTSIAPKTPSALEEVVLGASSDGTPILLGDVATVK
                     LALRKPVGVALVQDRPAMVMLLSREAGTNVLEVTREVHDVIGRLQQEKFDREGLQIEI
                     LADQVEYIEGALDLVEQNLLLGAILAVIVLFLFLRTFGASAVISVAIPVCAFATALGM
                     QALGRTINVVSLAGITFAVGMVVDNSIVALESIDTWRTRAPNTKLAAYLGIREVWGAL
                     IASTATTVAVFIPIVLWQGEVGELLRDVAVAIALAVSVSLVVSVLVIPSLAARLLSQG
                     KTLDIGGLTDFGRKFRSRVTEQVRWLCSSTQRSGATVAVAIIGSMLVTWALLPPMEYL
                     PTGNRNLVFGILVPPPGYSIDELESTGNRLQGEMARYTGQETDGEANIKRSFFVGSPD
                     RVFAGAVAEHDEDVGRMLDLLRRVQAKIPGMISFATQASLFGRTIGGGRVVEIDIQGP
                     DLQTLTAVGSRLFGDLRVALPGAQIRPEPSLDPGAPELHVIPRRREAARLQMGGAELG
                     LVVDALIDGAIIGEYTPTGQPKLDVVLRAVTDENRLLPDAAALLSAPVATPSGLTVPL
                     GALAEIEEELGPSVIRHLERRRAITLNVSPPEDLALEDAIDTIRNDVIGKLEAEGAIP
                     TGVELGLSGAAGKLELAQKQFGNILLLAVIICFLLIAALFEDFISPIAVLVSVPLAAA
                     GGAGGLWLVNALLGTQPLDLMTALGFLILIGVVVNNAILVVDGSIARLRSGSSLSDAV
                     PLAVEARVRPIFMTTATSLAGLLPMVIFSGSGSELYRGVGAIVLGGLLLSTVLTLFVV
                     PSAFTLLWKLRGHVDTDSKISS"
     gene            complement(4297..5052)
                     /locus_tag="VFG22_11515"
     CDS             complement(4297..5052)
                     /locus_tag="VFG22_11515"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HET6416915.1"
                     /translation="MERLQKVLARAGVASRRASEELILAGRVRVNGRIVTELGTKVDA
                     YKDRVEVDGKRLVAEKPVYYLIHKPREMVTTLDDPEGRATIADMLRRIPERVVPVGRL
                     DYHTSGALLATNDGDMVDALLRPVAGVPKIYAVKFQGHLDVEELDALRNGVKLDDGYR
                     TKPAEAFVLRQERNHTWVQLTLTEGKNRQIRRMGDAIHRRVMRLARQSFAGISIEGLR
                     PGEYRELNRDELQDLKKKFLNPYRRAHGASTEA"
     gene            5224..6312
                     /gene="corA"
                     /locus_tag="VFG22_11520"
     CDS             5224..6312
                     /gene="corA"
                     /locus_tag="VFG22_11520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HET6416916.1"
                     /translation="MGAPPGSIIPGPGVPRPEVHVIAYGPDRVDEYSVGHVSELKSLR
                     NGASAVTWINIVGLGDSETFSQLGEIFGLHALALEDVVNVHQRPKFEAYEGTDFIVLR
                     MPSLDPAINLEQVSMFVRDQVLITVQETPGDCLDGLRERIRNNKGRVRQRGVAYLAYA
                     VVDAIVEKFFPVVEHYTDRLEALEISVLATDTSNVVAEIHAIRHDLRTLRRAVAPTRE
                     VVGSMDRAEVGAEGEDIHVFLRDCHDHTIQLLDSLDTNRELASSLMDIHLSNVSMRMN
                     EVMKVLTIIATIFMPLGFIAGLYGMNFDGELSPWNMPELRWKLGYPFALALMGLTAIG
                     LAFFFRKKGWLGENGVEAERLPSENADR"
     gene            complement(6349..7032)
                     /locus_tag="VFG22_11525"
     CDS             complement(6349..7032)
                     /locus_tag="VFG22_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416917.1"
                     /translation="MKHWILCLSGLLVLGCGAKDPKPVTSPGAEQADSTTGGEVADGG
                     RCKPVGPGYEVSEYDTSGDNTPDVRKLFKTVGEGTLARLVLVCREADLNGDGRKDIVR
                     LYSDQGRPTREEADRDFNGRIDEVTQFSNGRVSLQEIDTSGNGTIDTRIFYEAGEPVR
                     AERDMAGRSTDAEWRPDRWEYYADGRTVRIGTDINGDGKVDRWDRDDERLRTSALAKQ
                     QTLGDSVKQ"
     gene            complement(7029..7715)
                     /locus_tag="VFG22_11530"
     CDS             complement(7029..7715)
                     /locus_tag="VFG22_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416918.1"
                     /translation="MSNFAHAVVGLAIMACLPAQGCKSGSSSTLPRGSMAEAQLCKGN
                     QVTATDVNEDGRVDIEQVSRNGRPFCTRADMNFDGKIDVERFYENDGERVSQERHDFD
                     FDGRIDQISFYENGELVRKELDTDFDNTIDTWLWCENGWVIRAERDRKHNGRVDVWES
                     YQQGLITEAQYDDNSDGRAEKWDSFRNGKLVLTKYDDNGDGEPDRSREMPLQSMGPAD
                     DALRCERGER"
     gene            7855..8049
                     /locus_tag="VFG22_11535"
     CDS             7855..8049
                     /locus_tag="VFG22_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HET6416919.1"
                     /translation="MARKKISTTVYITPEQNERLKLLHERTKVPVAVYIREGIDLVLD
                     QHQDELPGQMTLDATPPKKG"
BASE COUNT         1418 a         2565 c         2518 g         1564 t
ORIGIN      
        1 cttcgggtta tgagcccgac gagctaccag gctgctccac cccgcgggct gtttatagtc
       61 atgggcttcg acttgtcaaa acgagcccat gcactaaatt ttctcgatga attcgggcgt
      121 tttccggctg cttgcgtggc tgctgttagg gctatcatcg atggcccttg gctgcgaagg
      181 ggaggccccg gcgaagagcg gcgccagcgg gcctccgccg gcgcgggtct gggtcggcga
      241 agtgcgcacg ggaagcgtcc aagcggaatg gtcgttcctc ggtgacgtga gcgcactgca
      301 gcacgcacaa ctcgcggcag gtgcttccgg tgaggtgcgt cgggtgctcg tgcgcgttgg
      361 agaccgggtg aagaagggcg acttgctggt cgagatcgat ccctcgcttg cggcggcgcg
      421 cgttcgcgct gcatcggcct cgaaagaagc gggcgcggcg cagctcgagc gggcacagcg
      481 cgacgccgaa cgcttgagca cagccgggcc ggacatcgct gcgcgcgccg aaatcgaaca
      541 agccacttcg gagcgcaagc gcgccgcggc ggagggtggc cggctcaagg cagcggaggc
      601 ggaagcacgc gccgagctcg aacgccatcg catcacggcc ccgttcgacg gtgtggtcgc
      661 gaatcggcga gtcgacccgg gcgattgggt ggacccgggg gtcgaagcga tcgagctcat
      721 cgacgatcgt gatgtcgatg tgctcgcgtc ggttccgcct gaggtcgcga gcttcctctc
      781 cgtcggcgac aaggcaaacc tccggctcag gggcgaaacg acgccggcaa ccattcgagg
      841 catcgtccca gcgctcgaca gcgagtcgcg cactgtccgc ttgcgcttgg tgccggacga
      901 accgcctgtg tggctgttgc caggcgccgc agttgacgtg atcatcaccg tggcgcgcag
      961 cgaggaaggc gcactggtcg tgccgcgcga tgcattggtt tacgggattg cgaagacgca
     1021 gatcgtgaaa tcagtcgagg gaaaggcggc cccgatctcg gtcgaggtcc ttgcgagcgg
     1081 gcgagaagcg gttctgattc gggctgaaga gctctccgct ggcgatttgg tagtcacgcg
     1141 cggcaacgag cgcgtattcc cggggcaacc tctgatcgtg ctggaagact gagcgagtga
     1201 cgcacgacca gaaccatccg cctgaggagc aacccacagg gctttcgtgg ctcgcgatcc
     1261 agcggccgat cacggtcatc gtgggcatcg tgctggtcgt cctcttcggc acgctctcgg
     1321 tgatggacct gccaatccag ctcacacccg acatcgcaac gcccaccatc accatcaaca
     1381 ccgtttggcc gggtgccgca ccggtggaag tcgagacgga aatcctcgaa cctcaagaag
     1441 aggtgctcaa gaacgtgcag ggcctgaccc gaatggagtc gagcgcgagc atggacactg
     1501 gaagcatcac gctcgagttc gaggtcgaca cggtcatcga ggaagcgctc gtccgtgtga
     1561 ccaacaagct cagccaggtg tcgagttacc cggaggccgt gcgcgaaccg gtagtggaga
     1621 ccgccaatag cgcagggcca ccgctcgccg tcgtggcgat ccgagatccc caaaagggca
     1681 gcgtcgcggc ctacaggacc tgggttcagg acgagatcct cccggagctc gatcgtatcc
     1741 ccggcgtggc gggcatcttc ttcattggag gtcaggacac cgaggtgcac gtgctcttcg
     1801 acgcgcaagc cctcgccgcc cgcggtctct cggtgcaagg ggtagccgac cgcgtgcgga
     1861 gcgagcttcg tgatctatcg ggcggagacg tgacactcgg caagcgccgc tacctggtgc
     1921 gcacgtcgat cgcgcccaag acaccatcgg ctctcgaaga ggtggtgctc ggtgcgagca
     1981 gcgacggcac gccgattctt ctcggcgatg tggccaccgt aaagcttgcg cttcgaaagc
     2041 cggtcggcgt ggcgctggtt caggaccggc cggcgatggt gatgctcctt tcgcgcgagg
     2101 cggggaccaa cgtcctcgag gtcacacggg aagtgcacga tgtcatcggc cgcttgcagc
     2161 aagagaagtt cgatcgagaa ggtcttcaga tcgagatcct ggccgaccag gtcgaataca
     2221 tcgaaggggc gctcgatctg gtggagcaga acctcctcct cggcgcgatc ctcgccgtca
     2281 tcgtcctctt cctcttcctg aggacgttcg gcgcgtcggc ggtgatcagc gttgccatcc
     2341 ctgtctgtgc gtttgcgacc gccctcggga tgcaggcgct cggccgcacg atcaacgtcg
     2401 tgtccttggc aggcatcact ttcgccgttg gcatggtcgt ggacaactcc atcgtggcgc
     2461 tcgagagcat cgacacctgg cggacccgcg cgccgaacac gaagcttgcc gcatacctgg
     2521 gcatccgtga ggtctggggg gcgctgatcg cgtcgaccgc gacgacggtg gcggtgttca
     2581 ttcccatcgt gctttggcag ggcgaagtcg gcgagcttct gcgagacgtg gcggtggcga
     2641 tcgccttggc ggtctcggtc tcgctcgtgg tgtcggtcct cgtcatcccg agccttgcag
     2701 cgcgccttct ctcgcagggc aagactctcg acatcggcgg cctcactgac ttcggccgga
     2761 agttccgctc gcgggtcacg gagcaagtgc gttggctctg tagctcgacg cagcgttcag
     2821 gcgccacggt cgcagtcgcg atcatcggct cgatgctggt gacttgggcg ctcttgccac
     2881 ccatggagta cctgccgacg ggcaatcgca atctcgtctt cggcatcctc gtcccgccgc
     2941 ccggctactc gatcgacgag ctcgaatcga cgggcaaccg gcttcaaggc gaaatggccc
     3001 gctacacggg gcaagagacc gacggtgagg cgaacatcaa gcgcagtttt ttcgtgggaa
     3061 gcccggatcg ggtcttcgcg ggcgcagtgg ccgagcacga tgaagacgtc ggacgcatgc
     3121 tcgatctcct gcggcgcgtg caggcgaaga tcccaggcat gatctccttc gccacgcagg
     3181 cttcgctctt cgggcggacc atcggcggag gccgcgtggt cgagatcgac attcaaggcc
     3241 ccgacttgca gacgctgact gctgtcggta gccgcctctt cggagatctt cgcgtcgctt
     3301 tgccgggcgc gcagattcgg ccggagccca gcctcgaccc tggagcaccc gagctgcacg
     3361 tcattccgag gcgaagggaa gcggcccgac ttcaaatggg aggggccgag ctcgggctgg
     3421 tggtggacgc gttgatcgat ggcgccatca tcggcgagta cacgccgacc ggacagccca
     3481 agctcgatgt cgtcctgcgc gccgtgaccg acgagaatcg actgctcccc gatgccgccg
     3541 ccctactctc ggctccggtc gccactccga gcggcctgac cgtcccactt ggcgcgctcg
     3601 ccgagatcga agaggagctc ggcccctcag tcatccggca tctggagcga cgccgcgcga
     3661 tcacgctcaa cgtctcgcct ccagaggacc tcgcgctcga agacgcgatc gatacgattc
     3721 gcaacgatgt catcggcaag ctcgaagccg aaggtgcgat ccccaccgga gtcgagctcg
     3781 gactcagcgg agccgcaggc aagctcgagc tcgcacagaa gcagttcggc aacatcctct
     3841 tactggcggt gatcatctgc tttctcctga tcgcggcgct cttcgaggat ttcatatctc
     3901 cgatcgcggt cctggtgagc gtgccccttg ccgccgctgg cggtgcgggc ggcctctggc
     3961 tggtgaacgc cttgctcggc acgcagccgc tcgacttgat gactgccctc ggctttctaa
     4021 tcctcatcgg cgtggtcgtg aacaatgcga ttctggtcgt cgacggttcg attgcgcgcc
     4081 ttcgctcggg ctcgagtctc agcgacgcag tccccctcgc cgtcgaagcg cgcgtccgtc
     4141 caatcttcat gaccaccgcg acctcgcttg ctggcctcct cccgatggtg atcttctcgg
     4201 gctccggctc ggagctctat cgcggtgtcg gtgcgatcgt cctcggtggt ttgttgctgt
     4261 cgacggtgct cacgctcttc gtggttccga gcgcgttcac gcttctgtgg aagctccgtg
     4321 ggcacgtcga tacggattca aaaatttctt cttgagatct tgaagctcgt cgcgattgag
     4381 ctctcgatac tcacccggcc tcaggccctc gatgctgatg cccgcgaagg actggcgtgc
     4441 caatcgcatg acgcggcgat gaatcgcgtc gcccatgcga cgaatctgcc ggttctttcc
     4501 ctcggtgagc gtgagctgaa cccatgtatg gttccgctcc tggcgcagaa cgaacgcttc
     4561 ggcgggtttc gtccgatagc catcgtcgag cttcacccca tttcgaagcg catcgagctc
     4621 ctcgacatcg agatggcctt gaaacttcac tgcatagatc ttgggcaccc cggcgacggg
     4681 acgaagaagc gcatcgacca tgtcgccatc gttggtcgcc agcagcgcac cactggtgtg
     4741 gtagtcgagc cggccgacgg gcacgacgcg ctcggggatc cttcggagca tgtcggcgat
     4801 cgtcgccctt ccctctggat cgtcgagcgt cgtcaccatt tcacgtggct tgtgaatgag
     4861 atagtagacc ggtttctccg ccacgagccg cttcccgtcg acctcgacgc ggtccttgta
     4921 cgcatcgacc ttcgtgccaa gctcggtcac gatgcgtccg ttgactcgca cccgccccgc
     4981 caagatgagc tcctcggaag cacgccgaga agccacgcct gcccgagcca gcaccttctg
     5041 aagtcgttcc accggcggag cctagcacct ccgatcgcga acgcgacgcg gagcacgtcc
     5101 atctcttgac agcaagaacg gcaggacgtc agatggcggg gacttcgcaa ggaggctcca
     5161 atcgctaaga agacacatcg aaagcagaag cgcgccgcga ttcgcaggag gcgtccggag
     5221 atcgtgggag cgcccccggg gagcatcatt cccggtcctg gcgtgccgcg gcccgaggtt
     5281 cacgtcatcg cctacggtcc cgatcgcgtg gacgaatact cggttggaca cgtctccgag
     5341 ctgaagtcgc ttcgcaacgg cgcttccgcg gtcacttgga tcaacatagt cggcctcgga
     5401 gactcggaaa ctttctcgca gctcggcgag atattcggtc tccacgctct ggcactcgag
     5461 gacgtcgtca acgtgcatca gcgccccaag ttcgaagcat acgagggcac ggacttcatc
     5521 gtcctgcgaa tgccatcgct cgatccggcc atcaatctgg aacaggtgag catgttcgtg
     5581 cgcgaccagg tgttgatcac cgtacaagaa acgccaggcg actgcctcga cggcttacgc
     5641 gagcgaatcc gaaacaacaa aggcagggtc cggcagcgcg gagtcgcata cctcgcttac
     5701 gcggtcgtcg acgccatcgt cgagaagttc tttccggtag tggagcacta caccgatcgc
     5761 ctggaggctc tcgaaatcag cgtcctcgcg accgatacca gcaatgtcgt cgcggaaatt
     5821 cacgcgattc gccacgacct gcgaaccctg cggcgagctg tagctccgac gcgcgaggtg
     5881 gttggctcca tggaccgagc cgaggtcggc gctgaaggag aagacatcca cgtattcctt
     5941 cgcgactgcc acgaccacac gattcagctc ttggattcgc tcgacaccaa tcgtgagctc
     6001 gcctcgagcc tcatggacat ccatctatct aatgtgagca tgcgaatgaa cgaggtgatg
     6061 aaagttctga cgatcatcgc cacgattttc atgcccttgg gtttcatcgc cggtttgtac
     6121 gggatgaatt tcgacggaga gctctcgcct tggaacatgc cggagctccg ctggaagctc
     6181 ggttacccct tcgcgctcgc actgatgggc ctcaccgcga ttggcctcgc ctttttcttt
     6241 cgaaagaaag gctggcttgg ggagaacgga gtcgaggcgg agcgcttgcc gagcgagaac
     6301 gccgaccgat gacgacgcgt ggcagcgcgt catgctctcg acagaacgct actgcttgac
     6361 cgagtcgccg agggtctgct gcttggcaag agcggaagtc cgaagtcgct cgtcatcgcg
     6421 gtcccagcga tcgacctttc cgtcgccatt gatgtcggtg ccgatgcgca cggtgcgacc
     6481 atccgcatag tactcccagc ggtcaggacg ccactcggca tcggtgctgc gaccagccat
     6541 gtcgcgctct gcccgcaccg gttcgccggc ttcatagaag atccgggtgt cgatcgttcc
     6601 atttccgctg gtgtcgatct cttgaagact cacgcggcca ttgctgaact gcgtcacctc
     6661 gtcgatgcgg ccgttgaaat ctctgtcggc ttcttcgcgc gtggggcggc cctggtcgga
     6721 gtagagccgc acgatgtcct tccgtccgtc cccgttgaga tcggcctccc ggcagaccag
     6781 aacgaggcgc gcaagggtcc cttctccgac ggttttgaag agctttcgga catcgggcgt
     6841 gttgtcgccg gaggtgtcgt actcgctgac ttcgtagccc ggaccgacag gcttgcaccg
     6901 cccaccgtcg gccacttcac cacctgtggt cgagtcggcc tgttcggcgc ctgggctcgt
     6961 cactggttta gggtctttgg caccgcagcc gagtaccagc agaccgctga ggcaaagaat
     7021 ccaatgtttc atcgttcgcc cctctcgcac cgaagcgcat catcggcggg ccccatgctt
     7081 tgcaggggca tttcccgact gcgatcgggt tcaccatccc cgttgtcgtc gtacttggtc
     7141 aggacgagct tgccgtttcg aaacgaatcc catttctcgg cgcgaccgtc gctgttgtcg
     7201 tcgtactgag cctcggtgat gaggccctgt tggtagcttt cccaaacatc gacgcgacca
     7261 ttgtgcttac ggtcgcgctc ggctcgaatg acccagccgt tctcgcacca gagccaggta
     7321 tcgatcgtgt tgtcgaagtc cgtgtcgagc tcctttcgca ccagctcgcc gttctcgtag
     7381 aaggaaatct ggtcgattcg gccgtcgaaa tcgaagtcgt gtcgctcctg gctcacgcgc
     7441 tccccatcgt tttcgtagaa tcgctccacg tcgatcttgc cgtcgaaatt catgtcggct
     7501 ctcgtgcaga acggacggcc gttgcgactc acctgttcaa tatcgacacg cccgtcttca
     7561 ttaacgtcgg tggccgtgac ctgattccct ttgcacagct gcgcctcggc catggagccg
     7621 cggggcagag tgctcgagga gccgctcttg cacccctgcg ccggcagaca tgccatgatg
     7681 gcaagcccga ccaccgcatg cgcgaaattg ctcatgtttg cggtcacttc ttgggtcccc
     7741 cagaaccgga ttcccttaga atagggccca ccggcagcgc agcgcaagtc tctgacctag
     7801 tggattgaca atatatatgg tgccatgtag cttcatgcca cttctgaggt ccgaatggcg
     7861 cgcaagaaga tctccaccac ggtctacatc acccccgagc agaacgagcg gctcaagctg
     7921 ctacacgagc gcacgaaagt gcccgtggcg gtgtacatcc gcgaggggat cgatctggtt
     7981 ttagaccaac atcaggacga gcttccgggc caaatgacgc tagacgctac tccgcccaaa
     8041 aaggggtagc gacggcgcgt tgctg
//
LOCUS       DASRZF010000261         3525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2260129, whole genome shotgun sequence.
ACCESSION   DASRZF010000261 DASRZF010000000
VERSION     DASRZF010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2260129"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="VFG22_11540"
     CDS             complement(<1..782)
                     /locus_tag="VFG22_11540"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET6416920.1"
                     /translation="MKKSVLTVVALLLLSMAALGYFLLRPLSDEPVPADFQCYDLGQG
                     AYVPLDDPEQAFGVGLSYAGHIQETASSFDPMGAPPVFEKSPSALARSGAQVPFPSPE
                     ALTVAADSIEPGIAETLRSDFPELPPLLDYEVEMGFVLLEDIDPSQLDDPGFAPRLGF
                     FIANDLSARSIGILGEGRPNRYAYWGLSKSFPGFMPVGDKAWVPEKPVRNGIPCVEIE
                     SLVNGEVRQHQSTSDLIYTPVEMLHFVAAAFPNASLSKGTIVL"
     gene            complement(828..1295)
                     /locus_tag="VFG22_11545"
     CDS             complement(828..1295)
                     /locus_tag="VFG22_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416921.1"
                     /translation="MAAVSRRYRVAQILLTIGVLEFFGPIARDVSTTHLLNPSWVGHA
                     RFHLAWALVFMGLSGLCNLYLIWFRRPQLLSSLYISWAWQACHIFGFWGAAVLEGAYG
                     GSIVDPEFHFRVMGLNENVFAFVMLTAVLVVSIVYLKAALEPAVRAAAAGAGD"
     gene            complement(1536..2381)
                     /locus_tag="VFG22_11550"
     CDS             complement(1536..2381)
                     /locus_tag="VFG22_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416922.1"
                     /translation="MRLGIWTSTFLLAAILAQTVSASDSCTIMYRVKAALAVTNTRMG
                     KGDQIIDDLKGSLLIEYRQKQGAVVDGKVKILHYSMHERFKLESIVDVTTTLHHYAPS
                     CNGVAEPSWRLPSDPGFPKECRYTGSGHAVATGELDREAGKIEWAKCKAAPGYWGKAN
                     DAYTPAQKSKGKGCLNEMHVVGNIQCDGRLGCKFGGLASGNNPQFDVWNQPLIHGPAD
                     AESSVEISRDLSAIRTPKHRKDGYQSYNLPNDSPSRTWFSWTAIRDDSSSYTTCGKAD
                     AFGSD"
     gene            2526..2921
                     /locus_tag="VFG22_11555"
     CDS             2526..2921
                     /locus_tag="VFG22_11555"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HET6416923.1"
                     /translation="MSALEWLAATALMTALFWVPYVLERMVSLGVLGAMKPVDPEDEL
                     KQALWARRAKRAHYNGVENLTVFATLVLVAFAMGKGDAPGILTAAQVYFWARLIHFPA
                     GAFGMTGIRTVAFLVGFGAQVVVALRIFC"
     gene            complement(2952..>3525)
                     /locus_tag="VFG22_11560"
     CDS             complement(2952..>3525)
                     /locus_tag="VFG22_11560"
                     /inference="COORDINATES: protein motif:HMM:NF018696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6416924.1"
                     /translation="RAMELVELRAGDEVCLVGVGTGADLVYLPTGVNAVGVDLSEAML
                     AQAKRKLPIMGCEIELRVGDAQALPLEGEAFDAVILNLILSVVPDPTRCMSEAIRVLR
                     PGGRIVVFDKFLPDGSRPGLMRRLSNVFSTLLGTDINRRLGDIIAGLPCELVHDEPSI
                     LGGMYRVVLLRAALLGDRKVAEQERTGKIS"
BASE COUNT          704 a         1036 c         1083 g          702 t
ORIGIN      
        1 agcacgatcg tgcccttcga gagtgacgcg ttggggaaag cggcagcaac gaaatggagc
       61 atctcgaccg gtgtgtagat cagatccgac gtggactgat gctgacgcac ttcgccgttg
      121 accaggctct cgatctccac acaggggatg ccattccgca ccggtttctc tggcacccag
      181 gctttgtcgc cgacgggcat gaaaccggga aagctcttgg acaggcccca gtacgcataa
      241 cggttcggcc gtccctcgcc cagaatgccg atcgatcgcg cggagagatc gttggcgatg
      301 aaaaagccaa gtcgcggtgc gaagcccggg tcgtcgagct gcgacggatc aatgtcctcg
      361 agcaggacaa aacccatctc cacctcgtag tcgagcaaag ggggcagctc agggaaatcg
      421 cttcgaagag tttctgcgat cccgggctcg atcgaatccg cggccacggt cagggcctcg
      481 ggcgatggga aaggcacttg ggcgccagag cgtgccagag cacttgggct cttctcgaac
      541 actggcggcg cgcccatcgg gtcgaacgag gacgctgtct cttgaatgtg accagcgtac
      601 gagagaccga caccgaaagc ctgctcggga tcatcgagag gcacgtacgc gccctgcccg
      661 aggtcgtagc attgaaagtc ggcaggaacc ggctcgtcgg acagcgggcg aagaaggaag
      721 tagccgagcg ctgccatcga cagcagcagc aaagcgacca cggttaggac ggatttcttc
      781 atcgcggatg acacctttcg atggcttgtc gtagagactg caccgaatca gtcccccgcg
      841 cccgcagccg ctgcccgcac ggcaggctcg agggctgcct tgagatagac gatgctgacg
      901 acgaggacgg ccgtgagcat gacgaacgcg aacacgttct cgttcaggcc catcactctg
      961 aagtgaaact ccgggtcgac gatcgaacct ccgtacgctc cttcgagaac ggcggcaccc
     1021 cagaagccga agatgtggca ggcctgccaa gcccaggaga tatagagact cgaaagcaac
     1081 tggggacgtc tgaaccagat caggtaaaga ttgcagaggc ctgataggcc catgaagacc
     1141 agcgcccagg ccaaatgaaa ccgcgcgtgc ccgacccagc ttggattcaa gagatgtgtc
     1201 gtgctgacgt ctcgagcgat cggcccgaag aactcgagca cgccgatcgt gagaaggatc
     1261 tgggcgactc ggtagcgtct actgacagcg gccatacgac ctccattgag ttattcgttt
     1321 gaataactta tgagcataag acttgtcaag cgaaccactt catgcgagct tctgggcgct
     1381 gaaggcttga aattacaaag gaaacaagac ctcagtccag cggcacggtc cctctcctgc
     1441 cgtcgatcgc tgcgatgacc tccccgcgcc atcgcccgcg tcgaacagga gaaagcggcc
     1501 gttggcgaac accgcggtga acgattgggc ttaggtcaat ctgacccgaa tgcgtcggcc
     1561 ttgccgcagg tcgtgtagga gctcgagtcg tctcggatgg ctgtccagct aaaccaggtg
     1621 cggctcggag agtcgttggg caagttgtac gattggtagc cgtcttttcg gtgcttgggg
     1681 gtgcgtatcg ccgacaggtc tcgggaaatt tcgacggagc tttcggcatc cgctgggccg
     1741 tggatcagcg gctgattcca aacgtcgaac tgtgggttgt ttccggatgc aaggccgccg
     1801 aatttgcacc caagccttcc gtcacactgg atgttgccca cgacatgcat ctcattgagg
     1861 caacccttgc ccttgctctt ctgggcgggc gtgtaggcgt cattggcttt gccccagtaa
     1921 cccggcgcgg ctttgcactt ggcccactcg atcttcccgg cttctcgatc gagctcgccg
     1981 gtcgcgacgg catgcccact gcccgtgtac cggcactctt tagggaagcc tgggtctgaa
     2041 ggaagcctcc agcttggctc tgcgactccg ttgcagctcg gcgcgtagtg atgaagcgtc
     2101 gtagtgacat cgacgatgct ttcgagcttg aagcgctcgt gcatcgagta gtgaagaatc
     2161 ttgaccttcc cgtcgacgac ggcgccttgc ttctgtcgat actcgattag caatgagcct
     2221 ttcaaatcat cgatgatttg gtcgcccttc cccatgcgtg tgttcgtgac cgcgagagcg
     2281 gccttcacgc ggtacatgat cgtgcacgag tcagacgccg agaccgtctg tgcgagtatt
     2341 gcagctagca ggaacgtgga tgtccaaata cctaggcgca tttcaggcgc aagcttcgca
     2401 cagagcggtt cttggttcga tggttattga gtgcgccctg gacctttttc gggcactgac
     2461 gtaacaatcg gtcgcccctg tttgtacagg agccggcaga cgaaggttct gcgatggagg
     2521 atccgatgag cgcactcgaa tggcttgcag ccacggccct gatgacggca ctcttctggg
     2581 ttccgtatgt tctcgagcga atggtgtcct tgggcgtgct cggcgccatg aagccggtgg
     2641 atcccgagga cgagctgaag caggcgctct gggcacgtcg cgcgaaacgg gcgcactaca
     2701 acggagtcga gaaccttacg gtgttcgcga cattggtcct cgtcgcgttt gcaatgggca
     2761 aaggcgacgc acctggcatt ttgaccgcgg cgcaggtgta cttctgggcg cgtttgatcc
     2821 acttcccggc gggagcgttc ggcatgacgg gcattcgaac cgttgctttc ctagtgggct
     2881 tcggcgcaca agtggtcgtc gcgctgcgca tcttctgctg aacggcacga tgcgactcgt
     2941 cgtggctcca ctcaactaat cttcccggtc cgttcttgtt cggcgacttt tcgatcgccg
     3001 agcaaagcag cacggagcag cacgacccgg tacattcctc cgaggatgct tggctcgtca
     3061 tgcacgagct cgcatggcag tccggcgatg atatcgccga ggcgcctgtt gatgtcggtg
     3121 ccgagcagcg tcgagaacac gttcgatagc cgtcgcatca ggcccggtcg gcttccgtcg
     3181 ggtagaaatt tgtcgaacac gacgatgcgg ccaccgggcc tcaatacacg gatggcctcc
     3241 gacatacatc gggtggggtc gggaacgacg ctcaggatca gattgagaat cacggcgtca
     3301 aacgcctcgc cttcgagagg gagcgcttgc gcatcaccga ctcttagctc gatctcgcat
     3361 cccatgattg gaagcttcct tttcgcctga gcgagcatgg cttcgctcag gtcgacgccg
     3421 actgcgttca cgcctgtggg gaggtagact aggtcggcgc cggtcccgac accgacgagg
     3481 cacacctcat cgcccgcccg tagctccacg agctccatgg cgcgc
//
LOCUS       DASRZF010000262        11637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3982559, whole genome shotgun sequence.
ACCESSION   DASRZF010000262 DASRZF010000000
VERSION     DASRZF010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 11637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11637
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3982559"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..1176)
                     /locus_tag="VFG22_11565"
     CDS             complement(205..1176)
                     /locus_tag="VFG22_11565"
                     /inference="COORDINATES: protein motif:HMM:NF016819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HET6416925.1"
                     /translation="MLACALVTMSSCLSTAFAHPLAPSLIRIKEYAPGQAEVTWKTPR
                     RQVPGSKLNPVLPAHCNSLSEPQLLEQGTARIYTWRIDCGEHGFSNASIGADGIAESR
                     TDVIVHLELLDGRTYRSILTADEPRWTVPEAERNWDVFTSYARLGFEHIITGFDHLSF
                     VFGLMLLVSLRRLFWVVTAFTIGHSITLALSTLDVVRVQQRPVEILIALSIGVLAWEI
                     VRFANAERTGFAALPVMMAATFGLLHGLGFAGALRELGLPQGAIPLSLFSFNVGIEVG
                     QLAFITLALFPVVLLCSSDSWSRPFRIASAYVIGSFAFYWVFARAFA"
     gene            1326..2804
                     /locus_tag="VFG22_11570"
     CDS             1326..2804
                     /locus_tag="VFG22_11570"
                     /inference="COORDINATES: protein motif:HMM:NF015971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HET6416926.1"
                     /translation="MRTIPVTRTSARLVPDPTRVITKPFMPGSEHSVDGRSRVERILS
                     RILALSESEVRTTLSTTQARFSARHVDLRSVFETNFAAVAARHVARSNRLSAERKLLI
                     GAYFTHEYSIESAALSNPSIVPAPDQEGLAPGEQRFVLSLRAIGEGHISSIEFRSGVI
                     DAGGQIEMEAPSRRASTARHRAPVYEKHTFHTKLRELGIYNEIAATVLDALPERFSLE
                     QLETMIREVDQQHGHRPDAAHVTRTIHWLASSNYESSFASDSKISERVLFPTGPTESH
                     GMEDARFVRFTHDDGTVTYFAPYTAYDGYQILPQLIETADFVTFRIATLNGPSARNKG
                     IALFPRMIDGRYAALARLDNENNFLMWSDNVRFWHQSEAIQRPERPWEITQLGNCGSP
                     IETDAGWLVITHGVGAMRQYSLGAILLDKDDPARVIGHLAEPLLEPAADERDGYVPNV
                     LYSCGSMISGGLLVLPYGFSDVGTRIATVRLDDLLAELTRVR"
     gene            complement(2788..4065)
                     /locus_tag="VFG22_11575"
     CDS             complement(2788..4065)
                     /locus_tag="VFG22_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416927.1"
                     /translation="MSPDLRLSGYAPFRLGVNYWPARTAMGWWTAFDPAEVAADFGRI
                     AGCGFDSVRIFLTWEDFQPTPSRVDSTMIDRLVTTLDQARRAGLSVMPTLFTGHMSGV
                     NWIPPWALGEETGDDRFRVVSGGRVTASRLANWYSDASIVRAQSSLARELAGALAGHP
                     ALWAWDLGNENSNCAVPPDKGAAREWLLRVTDSIHGADPDVLVTLGLHMEDLEQDRNL
                     GPREAAEVCDFLQMHGYPGYATWADGPTDERVLPFLARLTRWLSGGADVLFAEFGVPT
                     ATSGEPDREPAIAASGPALVEEQEAASYVSRALDALRDCGTTGAMLWCYSDYAEAIWG
                     RPPLDLAVHERSFGLWRADASPKPTVAMVEAFAKRFATTDAGPSIADSAWIDIDVAEF
                     YRAPSSELQRLYGRYCEAKDALSAAPPNVNEPG"
     gene            complement(4062..5126)
                     /locus_tag="VFG22_11580"
     CDS             complement(4062..5126)
                     /locus_tag="VFG22_11580"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6416928.1"
                     /translation="MKSPSLDAPPALRVAVLAPISWRVPPHHYGPWEQFVSLLTEGLV
                     QRGLDVTLFATADSITKARLVGIAPRGYSEDVTIDAKVWECLHISAVFERAAEFDLIH
                     NSFDFLPLSFSRLVDTPVVTTIHGFSSERILPVYQKYNGRTHYVSISDADRHPSLEYA
                     ATIHHGIDMGAFAIGTGPREYLLFFGRIHPDKGTEEAILVARRAGLPLVIAGIIQDRG
                     YFERAVEPHIDGETVRFVGAVGPEGKSELLGGARALLHLVSFDEPFGFSVVEAMACGT
                     PVIANRRGSMPEIIAEGVNGRIVDSTADAVEAVAGIAELDTEAVRASVERRFDKDRMV
                     DEYITVYRRIVDGLASKSPA"
     gene            complement(5123..7435)
                     /locus_tag="VFG22_11585"
     CDS             complement(5123..7435)
                     /locus_tag="VFG22_11585"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6416929.1"
                     /translation="MSKDENPGAVQRVALLGNHLPRQCGIATFTTDLADAISSTFPGI
                     ECAVVAMNDAGRRHAYGDRVRFEIAESDLASYRRATDFLNVGGFDVLSLQHEYGIFGG
                     KAGSHVLALLSKVRMPIVTTLHTILGEPTLEQRLVMDEVTRLSSRLIVMSHCGGELLR
                     EVHGVPAEKIDFIPHGIPSLPSSSASKKRLGVEEMSLILTFGLLSPDKGIEYVIDALP
                     AIVARHPTATYVVVGATHPHVKEWHGEAYRRSLELRAHKLGVEEHVVFHDRFVSASEL
                     AEFLAATDIYITPYLNPEQITSGTLAYALGNGKAVVSTPYRYARELLADDRGLLVPFR
                     DSSAIGATVNALLDDRMELQARGARAAEYGRNMVWPSVARQYLTSFERAYQESATLRR
                     SFIASRTVTERLIDLPEITLQHLRALTDDTGMLQHALFSVPRYDDGYCLDDNARALLL
                     TSLIEDAGTDDRPTTRALSSRYLAFMSHAFNQDSGRFRNFMSYSREWLEDCGSEDSHG
                     RALWALGAVVGRAREPGTKSLGSELFHAALPAVKELESSRAWAFALLGIHEYLRAFRG
                     ESAVEVLQKQLSEQLLSRFQTHGADDWPWCEDVIAYDNARLPQALIVSGKQMGNREMV
                     AAGLESLRWLNELQRSEEGYFAPIGSNGFYPRHGERAHFDQQPLEACATISACLDAWR
                     VTGDEEWPREMWRAFSWFLGENQLQAPLYDPTTGGCLDGLHPDRPNENQGAESTLSFL
                     IALTEMSALDSEKQLRDENAPSSPTGEGSG"
     gene            complement(7449..8603)
                     /locus_tag="VFG22_11590"
     CDS             complement(7449..8603)
                     /locus_tag="VFG22_11590"
                     /inference="COORDINATES: protein motif:HMM:NF024303.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HET6416930.1"
                     /translation="MEAKQAAVEVLLHNASGPVAGLPRTAGWGYPEPYTRDLMLSAFG
                     ILVSGSEELVETLGRTLTVLAQNQTQHGHIPSMVHDPDNLGSSDSTPLFLIAVGLYQR
                     VTGNDGFLDAAVQKALTWMQYQSPDDTVMVGQLPTSDWRDEHWVLGYGLYVNSLVHTY
                     LKLHGRDEEAGKLSSLMNRLDVRRDVGNHHIHEGLVVPSKPYFAIYAYKVYNNERFDL
                     LGNCLASLTGIASKSRSERIVQWVEAECAALREQGELAVDLPPCLLPYIRGDDPDWRP
                     RYEGFNQAGEYHNGGIWPFICGLYVATCVAAGRHDLARRKLLALTELVTLHRRESLTW
                     GFNEWIKAQTGKPMGCDWQSWSAAMYLYAVACVERRSTPFFDELRIGTET"
     gene            complement(8665..9807)
                     /locus_tag="VFG22_11595"
     CDS             complement(8665..9807)
                     /locus_tag="VFG22_11595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6416931.1"
                     /translation="MKALILYDYPPSPAGLATQGDLLYRGLLELGVDAHAANFEAPQE
                     KEWYYRWFEPDVVVGIGYWGHTPHLIQHPQRYGARAVPWFVADGYVANYQEVLNDLPL
                     ILVTSNWVKETYVRDGVRPDIIEVLPVGCETERFMPRDRNDPKVRAIRETLGVAEDQI
                     FILTVGGDAASKGAQEVMQALAKIDVEAPDWKYVCKVWPQPRTDEQNLLDLQLAAHLG
                     IDKNVTISTGRFSRNIMPYLLAACDIYAAPSRLEGFGMIQVEANACEKPVIAIDAMAF
                     RDTMVHGETAFLAGVAKEIMISEAVLGEDQGFQPNSSVSFGKPRTSDYRASVNDIATH
                     LLTLMRDAELRKRMGKAGRHRVVERFDYRIVASRFVEIIQNRLGIR"
     gene            complement(9804..10448)
                     /locus_tag="VFG22_11600"
     CDS             complement(9804..10448)
                     /locus_tag="VFG22_11600"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HET6416932.1"
                     /translation="MPTETPLRVAVVVAHPDDETLWCGGLILQHSDWHWRIVTLCRGS
                     DQDRAPRFDGVLSLFGAKGEMADLDDGPDQRPLPASEIEETILRLLPQREFDLILTHG
                     PRGEYTRHRRHEECCRGVSMLWQAQRLSTRGLWMFAYEDRGGNGPPLVRSDADRREVL
                     PPEVWREKRRVLTDLYGFTDESWEVRVAPREEGWWCFDEATSAHEHVQRWSSDA"
     gene            complement(10449..10577)
                     /locus_tag="VFG22_11605"
     CDS             complement(10449..10577)
                     /locus_tag="VFG22_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416933.1"
                     /translation="MGDKSPKQKNRKANQKQQTKKDDKQRQAAASEVKKAALAGKK"
BASE COUNT         2184 a         3583 c         3654 g         2216 t
ORIGIN      
        1 gcatccgagc aacgccatcg aaaaggcaaa tgcgatcaag gggctcgcac ttgctacaaa
       61 ggatggactc gtcattgtgt gctcaaaatg gggtgaggtt ctcgccggta ccggggcgaa
      121 gatcagtatt ggtgcgtaac atgtcaatag cagtcagagc ggacgactgc ttgaacgttc
      181 ttgtcgccct ttataaccga ttattcaagc gaatgctctg gcgaaaaccc agtagaatgc
      241 gaacgatccg atgacatagg cgctggcaat ccgaaacggt cgcgaccacg aatcggacga
      301 gcagagcagc accactggaa agagcgcaag cgttataaag gcgagctgcc ccacttcgat
      361 tccgacgttg aaagagaaca acgaaagcgg aatcgcccct tggggtagac cgagctcgcg
      421 cagtgcgcct gcaaagccga ggccatgcaa caggccgaag gtcgccgcca tcatcaccgg
      481 aagggcggca aagcctgttc gttcggcgtt ggcaaagcgc acgatttccc atgccaagac
      541 gccgatgctc agggcgatca agatttcgac gggtcgttgt tgaacgcgga cgacgtccaa
      601 ggtggataat gcaagggtga tgctgtgtcc gattgtgaat gccgtcacga cccagaagag
      661 acgtcggagg ctgaccaaca acatcaaccc gaatacgaag gagaggtgat cgaatcctgt
      721 aatgatgtgt tcgaacccaa gtcgcgcgta gctcgtgaac acatcccagt tgcgttcagc
      781 ttccggcacg gtccaccggg gttcgtcagc cgtgagaatg gatcggtagg tgcggccatc
      841 caggagctcg agatgcacga tgacgtctgt cctcgattcg gcgatgccgt ctgccccgat
      901 cgatgcgttc gagaagccgt gctcaccgca gtcgatgcgc cacgtgtaaa tgcgtgccgt
      961 tccctgttcg aggagttgcg gttccgagag cgaattacag tgagcaggaa ggacagggtt
     1021 gagcttgctt cccgggactt ggcgcctcgg ggtcttccat gtgacctccg cctggccggg
     1081 cgcgtattct ttgatgcgta tcagcgacgg cgccaacggg tgcgcgaatg ccgtcgacag
     1141 gcaagacgac atcgtgacca aggcgcatgc gagcatcgct ggtgcggacc acgagcctcg
     1201 ggagcagcct tccattggat cgagtatgga caagaacaag cgcctcgcac atccagccat
     1261 catggcgagg tcgttttcct gtgtttccgc tggccacgcc tcctcagtgc ggggagactg
     1321 tcgagatgcg caccatccct gtcacgagga cctccgcgcg gttggtgccc gatccgaccc
     1381 gggtcattac gaagccgttc atgcccggaa gcgaacactc ggtggacgga cgctctcgcg
     1441 tcgagcgcat tctatcgagg atcctggcct tgtccgagtc cgaggtgagg acgaccttga
     1501 gcacgacgca agcccgattc tccgcgcgtc acgtcgactt gcggtcggta ttcgagacga
     1561 acttcgcggc cgtcgcggcg cgacatgtcg cccgatcgaa ccggctttcg gcagagcgca
     1621 agctgctcat cggcgcctat tttacccacg agtactcgat cgagtctgcc gcgctgagca
     1681 atccttcgat cgtgccggcg ccggaccaag agggtctcgc tccgggcgag cagcgctttg
     1741 tcttgagctt gcgggccatc ggcgaggggc atatctcgtc gatcgagttt cgctccggtg
     1801 tcatcgacgc gggcggccag atcgaaatgg aagcgccgag tcggcgagcg agcaccgcgc
     1861 gccaccgggc tccggtgtac gagaagcaca ccttccacac caaacttcgt gagctcggca
     1921 tctacaacga gatcgccgct accgtgctcg acgcgcttcc cgagcggttt agcctagagc
     1981 agctcgagac catgatccgg gaggtcgacc agcaacacgg tcacagaccc gacgccgcgc
     2041 acgtcacccg gaccatccac tggcttgcgt cctcgaacta cgagagctcg ttcgcctcag
     2101 actccaagat ctccgagcgt gtgctctttc cgactggccc gaccgagagt cacgggatgg
     2161 aggatgctcg cttcgtccgt ttcacccatg acgacggaac agtgacgtac ttcgctccgt
     2221 atacggcgta cgatggctat cagatcctcc cgcagctgat cgaaacagcc gactttgtga
     2281 ctttcaggat cgcgacgctg aacggcccgt ccgccaggaa caagggcatt gcgctctttc
     2341 cccggatgat cgacgggcgc tatgctgcgc tcgccaggct ggacaacgag aacaacttcc
     2401 tcatgtggtc ggacaacgtc cgcttctggc accagagcga ggcgatccag aggcccgagc
     2461 gtccctggga aatcactcag ctcggaaatt gcggatctcc tatcgaaacc gatgcgggct
     2521 ggctcgtgat cacgcacggc gttggggcaa tgcgacaata ttctctcgga gccatcctgc
     2581 tcgacaagga cgaccctgca cgcgtcatcg gtcacctcgc cgagcctctg ttagagcccg
     2641 cggcagacga acgcgatggc tacgtgccca acgtcctcta ctcgtgcggc agcatgatct
     2701 cgggcgggct cctcgtgctg ccctacggtt tctccgacgt cggcactcgc atcgcgacgg
     2761 tgcgcctcga cgaccttctc gcggagctca cccgggttcg ttgacgtttg gaggcgccgc
     2821 gctcagcgca tccttcgcct cgcagtagcg tccgtatagg cgctgcagct ctgacgaagg
     2881 tgcgcggtaa aactcagcga catcgatgtc gatccaggcc gagtcggcaa tggaaggacc
     2941 ggcgtcagtc gtggcgaacc gcttcgcaaa tgcctcgacc atagccaccg tcggctttgg
     3001 ggaggcgtcg gcacgccaca acccgaacga gcgctcgtgg accgcgagat ccagtggggg
     3061 acgtccccag atcgcctccg cgtagtcgga gtaacaccag agcatggcgc cggtggtacc
     3121 gcagtcgcgc agcgcgtcga gggcccggct gacgtacgaa gcagcctcct gctcttcgac
     3181 gagggcgggc ccggacgcgg cgatggccgg ttcacggtct ggctcaccgc tcgtggcggt
     3241 tggcacaccg aactctgcga agaggacgtc ggcgccgccg ctcagccatc gcgtcaaccg
     3301 agcgaggaac ggcaagaccc gctcatcggt ggggccgtcc gcccacgtgg cgtaaccggg
     3361 atacccgtgc atctgcaaga agtcgcagac ctcggcggcc tcgcgcgggc cgaggtttcg
     3421 gtcctgctcg aggtcttcca tgtggagccc gagcgtgact agaacgtcgg ggtcggcgcc
     3481 gtggatggag tccgtcaccc gcaagagcca ctcgcgggcg gcgcctttgt ccggggggac
     3541 tgcgcagttc gagttttcgt ttccgaggtc ccatgcccat agagccggat gccctgcaag
     3601 cgcgccggcg agctcacgtg cgagcgacga ctgcgctcga acaatggacg cgtcagagta
     3661 ccagttcgcg aggcgcgacg ccgtgactcg gcctccggag acgacgcgga atcgatcgtc
     3721 acccgtctct tcaccaagag cccagggagg gatccagttc acgccgctca tgtggccggt
     3781 gaagagcgta ggcatgaccg aaagtccggc gcggcgggcc tggtccaggg tcgtgacgag
     3841 gcgatcgatc atcgtcgagt cgacgcgcga aggggttggt tgaaagtcct cccaagtgag
     3901 gaagatgcgg acggagtcga atccgcaccc tgcgatgcga ccgaagtccg ccgcaacctc
     3961 cgccgggtcg aatgcggtcc accagcccat cgccgtccga gccggccagt aattcacgcc
     4021 cagccggaag ggcgcgtacc cgctcaagcg caggtctggg ctcacgcggg gctcttggac
     4081 gctaagccgt ctacgatgcg ccggtagacc gtgatgtact cgtcgaccat ccggtccttg
     4141 tcgaatcggc gctcgaccga tgcgcgcacg gcctcggtgt ccagttcagc gatcccggct
     4201 acggcttcca cagcgtcggc ggtcgagtcg acgatgcggc cgttcacgcc ctccgcgatg
     4261 atctccggca tggatccgcg tcggttggcg atcaccgggg tcccgcaggc catcgcttcg
     4321 accacgctga agccgaacgg ctcgtcgaag ctcacgaggt ggagcagggc gcgcgcgccg
     4381 ccgagaagct cgctcttccc ctcgggcccg accgcgccga cgaaacgcac cgtctcgccg
     4441 tcgatatgag gctcgaccgc gcgctcgaaa tagcctcgat cctggatgat gccggcgatc
     4501 acgagcggca gacctgcgcg ccgagcgacg agaatggctt cctccgtgcc cttgtccgga
     4561 tgaatgcggc cgaagaaaag gagatactcg cgtgggccgg taccgatcgc gaacgcgccc
     4621 atgtcgatac cgtggtggat ggtggccgcg tattcgagcg aagggtgtcg gtcggcatcg
     4681 ctgatggaga cgtagtgggt gcgcccgttg tacttctgat agacggggag gattcgctcg
     4741 gaggaaaaac cgtggatcgt ggtgacgaca ggcgtgtcca cgagccgaga gaagctgagg
     4801 ggcaagaagt cgaagctatt gtggatgagg tcgaactcgg cggcgcgctc gaatacggcc
     4861 gagatgtgca gacactccca gaccttcgcg tcgatggtca cgtcttcgga gtatccgcgt
     4921 ggcgcgatcc cgacgagccg agccttggtg atcgagtcgg cggtggcgaa gagggtgacg
     4981 tccaatccac gctgcaccag accctcggtc aagagagaga cgaactgttc ccatggtccg
     5041 tagtggtgcg gagggacgcg ccaagagatc ggggcgagca ccgccacgcg gagcgcgggc
     5101 ggggcgtcga gcgaaggcga cttcacccgc tgccttcgcc ggttggagag gagggtgcgt
     5161 tctcgtcgcg cagctgcttc tccgagtcga gcgcgctcat ctcggtgagc gcgatcaaga
     5221 acgagagtgt cgactcggcc ccttggtttt cgttcggtcg atctgggtgg agcccgtcga
     5281 ggcagccccc ggtggtgggg tcgtagagtg gcgcttggag ttggttctca cccagaaacc
     5341 aactgaacgc gcgccacatc tcgcgcggcc attcctcgtc cccggtgacg cgccacgcgt
     5401 cgaggcaagc ggatatcgtg gcgcaggcct cgaggggctg ctggtcgaag tgcgccctct
     5461 cgccgtgtcg cgggtagaat ccgttcgagc cgatcggggc gaaatagcct tcctccgagc
     5521 gctggagctc gttgagccat cggagggact cgagtccggc cgcgaccatt tctcgattac
     5581 ccatctgctt gcccgagacg atcagggcct gcggcaggcg cgcgttgtcg tatgcgatga
     5641 catcctcgca ccacggccag tcgtcagcgc cgtgggtctg aaatctcgac aggagctgct
     5701 cggatagctg tttttgcaag acctcgaccg cgctctctcc tcgaaacgcg cgcagatatt
     5761 cgtggatacc aaggagcgcg aaagcccaag ccctcgagct ctcgagctct ttgaccgccg
     5821 gcagcgcagc gtgaaacagc tcgctgccga ggctcttagt cccaggttcg cgagcgcgac
     5881 ccaccacggc gccgagcgcc caaagtgcgc gaccatgact gtcctcactg ccgcagtctt
     5941 cgagccattc ccgggaatac gacatgaagt tccggaaccg tcccgagtct tgattgaacg
     6001 cgtgactcat gaatgcgaga tagcgcgaag acaaagcgcg agtggtcggt cgatcgtcgg
     6061 tgcccgcgtc ctcgatcaag gacgtcagga gtagggcgcg tgcgttgtcg tcgaggcagt
     6121 acccgtcgtc gtagcgtgga acggaaaata gagcgtgctg gagcatgcca gtgtcgtccg
     6181 tcagcgcgcg caggtgctgg agagtgatct cgggcaggtc gatcaagcgt tctgtgactg
     6241 ttctcgacgc gatgaaagag cggcgcaagg tcgcgctttc ctggtaagcc cgctcgaagc
     6301 tcgtgaggta ttgccgcgca accgagggcc agaccatgtt gcgcccatat tcggccgcgc
     6361 gcgcgcctcg tgcttgtagc tccatgcgat cgtcgagcaa cgcgttcacc gtcgccccta
     6421 tcgcagacga gtctctgaac gggacgagga ggccgcggtc gtccgccagg agctcgcgcg
     6481 cgtatcgata aggagtcgaa acaaccgctt ttccgttccc caacgcgtag gccagtgtcc
     6541 cggaagtgat ctgctccggg tttaggtatg gggtgatgta gatgtccgtc gcagccaaga
     6601 actcggcgag ctcggacgcg ctcacgaagc gatcgtgaaa gacgacgtgc tcctccacgc
     6661 ctagcttatg agcccgcagc tcgaggctgc gccggtaggc ctcgccgtgc cactccttca
     6721 cgtgagggtg cgtggcgccg acgacgacat aggtcgcggt cgggtgccgc gcgacgatag
     6781 cggggagcgc atcgatgacg tactcgatcc ctttgtccgg agagaggagg ccaaaggtga
     6841 ggatcagcga catctcctcg acacccaggc gcttcttgct ggccgaactg ctcggcaagc
     6901 tcgggatgcc gtgagggatg aagtcgatct tttcggccgg caccccgtgc acttcgcgca
     6961 agagctcgcc gccgcagtga ctcatcacga tcaaacggct cgagagccga gtgacctcgt
     7021 ccatcacgag gcgttgttcc aaagtgggct cgccgaggat ggtgtgaagc gtcgtgacga
     7081 tcggcatgcg caccttgctg aggagcgcga ggacatggct gcccgccttg cccccgaaga
     7141 tgccgtactc atgttggagg gacagaacgt cgaacccgcc cacgttcaag aagtcggtcg
     7201 cgcggcggta cgacgcgaga tcgctctcgg cgatctcgaa tcgaactcga tcaccgtagg
     7261 cgtggcgtct accagcgtcg ttcatcgcga cgaccgcgca ctcgatgccc gggaacgtcg
     7321 acgagatcgc atcggcgagg tccgtcgtga acgtggcgat cccgcactgc cgcggaaggt
     7381 ggtttccaag cagcgctacg cgctggactg cgccagggtt ttcgtctttg ctcatcggcc
     7441 cattccgttc acgtttctgt ccctatgcgg agctcatcga aaaacggtgt gctccgccgt
     7501 tcgacgcacg ctaccgcgta tagatacatc gcggcagacc agctctgcca gtcacagccc
     7561 atcggtttgc cggtttgggc tttgatccac tcgttgaagc cccatgtgag cgactcccgc
     7621 cgatgcagcg tgacgagctc tgtcagggcc aacagcttcc gccgtgcaag gtcgtggcga
     7681 cccgcagcca cgcaagtcgc cacgtacaat ccgcaaatga aaggccagat cccgccgttg
     7741 tggtactcac cggcctggtt gaatccctcg tagcgcggtc gccagtcggg gtcgtctcca
     7801 cgtatgtatg ggagcagaca tggcggcaga tcgacggcta gctcaccctg ttccctcaga
     7861 gcagcgcatt cggcttctac ccactggact attcgttcgc tgcggctttt ggaagcgata
     7921 ccggtgagtg acgccaggca gtttccgaga agatcgaagc gctcgttgtt gtagaccttg
     7981 tacgcgtaga tcgcgaagta tggtttggag ggtacgacaa gcccctcgtg gatgtggtgg
     8041 ttgccgacgt cccgccgcac gtccagccgg ttcatgagag agctgagctt cccggcctcc
     8101 tcgtctcggc cgtgaagttt gagataagta tgaaccaagc tgttcacata caggccgtat
     8161 ccaaggaccc aatgctcatc gcgccagtcg ctggtaggga gctgacctac cattaccgta
     8221 tcgtccgggc tctgatattg catccaggtc agagccttct ggactgcggc gtcgaggaac
     8281 ccatcgtttc cggtcacgcg ctggtagagg ccgacagcga tcagaaacaa gggtgtcgag
     8341 tcgctagacc caaggttgtc ggggtcgtgg accatcgatg ggatgtggcc atgctgggtc
     8401 tggttctgag ccagcacggt gagtgtccgg cccagagtct cgacgagctc ctcgctgccg
     8461 ctgaccagga ttccgaaagc cgagagcatg aggtcacgcg tgtagggctc ggggtaaccc
     8521 catcccgcgg tgcggggaag accggccact ggaccggacg cgttgtgcag gagaacctcg
     8581 acagccgcct gcttcgcctc catgaccgat ttcggtaggg ggtcgtcgcc acgcttggtg
     8641 ctttgagaga cagctttcac ggcgttagcg aatgcccaat cgattctgga tgatctcgac
     8701 aaagcggctc gcgacaattc gatagtcgaa tcgttcgacc acgcggtgtc gcccagcctt
     8761 gcccattcgc ttccgcagct cggcgtcgcg catgagtgtt agcagatggg tggcgatgtc
     8821 gttgacgctg gcgcggtagt ccgatgtccg gggcttgcca aaggacaccg aggaattcgg
     8881 ctggaaacct tgatcctccc caaggacagc ctcgctgatc atgatctcct ttgccacgcc
     8941 ggcaaggaat gcggtctcgc catggaccat cgtgtcgcga aaagccatcg cgtcgatggc
     9001 gatgaccggt ttctcgcagg cattggcttc cacctgaatc ataccgaaac cctcgaggcg
     9061 agagggcgca gcatagatgt cacaagccgc gagaaggtaa ggcatgatgt ttcgcgagaa
     9121 acgcccggtc gagatggtga cgttcttgtc gatcccgaga tgcgccgcga gctggagatc
     9181 gagcagattc tgctcatctg tacggggttg gggccatacc ttgcacacgt acttccagtc
     9241 gggagcctcc acgtcgatct ttgcgagtgc ctgcatcacc tcttgagcac ctttggaggc
     9301 ggcatcgcca cccaccgtca gtatgaaaat ctggtcctcg gccacaccca gcgtttctcg
     9361 gatggcgcga acctttggat cgtttcggtc ccgtggcatg aagcgctcgg tttcacagcc
     9421 taccgggagc acctcgatga tgtcgggtcg tacgccgtcg cgaacatagg tctccttgac
     9481 ccagttcgag gtcaccagga tcaggggtag atcattgagc acctcctggt agttggcgac
     9541 gtagccatct gctacgaacc aggggaccgc tctcgcaccg tagcgctgcg ggtgttggat
     9601 cagatggggc gtgtgtcccc agtatccaat acccacgacg acgtcgggct cgaaccagcg
     9661 atagtaccat tccttttcct gcggcgcctc gaagttcgcg gcgtgcgcgt ctacgccaag
     9721 ctcgagcagt ccgcggtaca aaaggtcgcc ctgcgtcgca aggccagccg gactcggggg
     9781 ataatcgtaa aggatcaaag ccttcatgcg tcagagctcc atcgttgcac gtgctcgtgg
     9841 gctgatgtgg cttcgtcaaa gcaccaccag ccctcttcgc gcggtgcaac tcgtacctcc
     9901 cagctctcgt cagtgaagcc gtaaagatcg gttaagactc gtcgtttctc gcgccaaacc
     9961 tcagggggaa gcacttcacg tcggtcagcg tccgatcgta ccaagggcgg gccgttgccg
    10021 cctcggtcct cgtacgcaaa catccaaagg cctcgcgtcg aaagccgctg cgcttgccac
    10081 aacatcgaca ctcctcggca gcactcctca tgccgacgat ggcgggtata ttcgcctcgc
    10141 gggccgtgcg tgaggatcag gtcgaactcg cgctgcggca acagtcgtag gatcgtctcc
    10201 tcaatctccg aagccggcaa cggccgctga tcggggccgt cgtctagatc agccatttcg
    10261 ccctttgccc caaacaggct cagaacgcca tcgaatcgcg gcgcccggtc ctggtcggag
    10321 ccgcggcaca gggtgacgat acgccagtgc cagtccgaat gctggagaat gagcccgcca
    10381 caccagagcg tctcgtcatc ggggtgggcg acgacgacag cgactcgaag tggtgtctcc
    10441 gtcggcactc acttctttcc ggcgagtgct gccttcttga cctcagacgc tgccgcttgg
    10501 cgctgtttgt cgtccttttt cgtctgttgc ttctggttgg ccttgcggtt tttctgtttc
    10561 ggggatttgt cgcccatggt gcacctccta tgctcggaag cgagttccag atggcgcgcc
    10621 gccggataag cgcactgtcg cacggggctg ccggcttgtc ttgccgattt caatccggtg
    10681 cgactcgacc gatctgggct tccccggcgc tgccgcacat tacgtggtac tgtccctttg
    10741 tgagcgacac acggataggt gccattttgc gaagcgcttg gaagatcctc cggcttgatg
    10801 caatccgtcc cgtcgtccgc caaacgattg ctgatctcga ggcattgact gctcggcaag
    10861 tggtggggcg ggagccttcg cgatccgaga tcgaagtaga gacaggctca ctccgtgcgg
    10921 ctctcgatgc gtgttgcgac gagcttcgcg aaggctgcgg gctacccacg tcacccgggc
    10981 cgtcagcgca agctcagaga atgcatgccg ccgatgccga ctggaccgaa agctattcga
    11041 acgccctaga ggtctatcgc agcgcggcgt gcaaggagct ggatgcttcc cttgctgccc
    11101 tagcgttgcc tccggagcgc tcacccgcgt tagaaacgct gtagctaccc tcacctaccc
    11161 tcacgttgct gtggcgatgt gaaatcgaat gatatggtgt gacggcccgt gaagatggcg
    11221 atgcgggcaa gtccggttac tgggtcaaga agctacgcag caatcaccac cttcatcggc
    11281 atgccaagct ccaccgaata acgctgttcg taacgggcct cagggacgcg accttgcggg
    11341 tgcataaccc gaaggtcgtc acgccttctc gtctggtttg ggagcgccgt catcgatgcc
    11401 tgaatgcaaa aagtcgcttt gccgtttcgc ctagcgtttc cctcgtcatc tcttggccgt
    11461 tgccgcgtcg tccgctccga aaatccggac ctagtgcgat agggctacgc atatcgcccc
    11521 cccgacgggc gacgatggcg cccgcttgga gacaatcctc gtgcgcgttt cgatttcgct
    11581 ctcgcagagg gcttgtacgg cgtccttgtc ttgcctggat atggcataaa cacagca
//
LOCUS       DASRZF010000263         2216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1227974, whole genome shotgun sequence.
ACCESSION   DASRZF010000263 DASRZF010000000
VERSION     DASRZF010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1227974"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="VFG22_11610"
     CDS             complement(<1..611)
                     /locus_tag="VFG22_11610"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET6416934.1"
                     /translation="MQGLLGGGGWVRVLFALAVLLVSAKALAEEATPAVIVITAGEDA
                     TSGAAIRIDGEPSGTVPVRTTLSPGRHLVQVGKRGFVTFSKWMDLIGGQVLTIPVVLQ
                     RQVAKTGSLLVTADVTGMPVFIDGQQRGGTPLVVDGLSEGEHLVEIRSPGEGYQPFSE
                     TVTIRASERTTVEATIRIAPDLGSLRVITNVPGAIISLDGIDIG"
     gene            complement(752..>2216)
                     /locus_tag="VFG22_11615"
     CDS             complement(752..>2216)
                     /locus_tag="VFG22_11615"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HET6416935.1"
                     /translation="LRDFELGLRGEADGWFRITVDGQTSNKGTLDVDLRVMPTEARAD
                     GMVRIRGISLALIAPFAPDLPLYNTEVGTVSAEIELAASSPQQVRIEGRLELRELALA
                     SERIASEPVEHVNLDVSGKGVWHPDERRLQIERGRVRMGEAQMLFDGELERTPDHYRV
                     DLTAKLPPTRCNDVVGAIPADVLDSLSQFEWSGNWSGIAHVSLDSRTLEETDLSIRVR
                     NLCEFERAPSWVRVERFQGPFRHRAIEPDETVFEMRTGPGTEYWVAFADISPFVVPAV
                     ISHEDGAFYEHGGFAPWAIRDALVRNLQEGRYVVGASTISMQLAKNLYLQREKTLARK
                     VQEVILTWWLENALSKDQILELYLNVIEYGPSVYGLRHAAVYYFGREPSELSPAESAF
                     LACMLPSPKRYHVSYERRTLTRSMKSRMRRLLEHMAKRERIGPEALAYGLAEIEDFHF
                     RAGGDPLPPPRALPPLGAPENPDPEALDPFENLFVDP"
BASE COUNT          433 a          702 c          684 g          397 t
ORIGIN      
        1 ccgatgtcga ttccatcgag actgatgatc gcaccgggca cgttcgtgat cacgcgcaga
       61 ctgcccaggt caggtgctat gcgaatcgtc gcctcgaccg tcgtccgctc gctcgcccgg
      121 atcgtcacgg tctcggagaa cggttgatag ccctcgcccg ggctgcggat ctcgaccagg
      181 tgctcaccct cgctgaggcc atcgacgacc agaggggtgc ccccacgctg ctgtccgtcg
      241 atgaagaccg gcatccctgt gacgtccgca gtgaccagga gcgagccggt cttggccacc
      301 tgacgttgaa gcacgaccgg aatcgttaga acttggccgc cgatcaagtc catccacttc
      361 gagaaagtca cgaagccgcg tttaccaact tgcaccaggt gtcgaccagg gctgagcgtc
      421 gtgcggactg gaacggtgcc agagggctcg ccatcgatgc gaatcgccgc gcctgaagtg
      481 gcgtcttcgc ctgccgtgat cacgatgacc gcgggtgtcg cctcctcagc caaggccttg
      541 gcggaaacca gaagcaccgc gagcgcaaat agaacccgca cccaaccacc accgccaagc
      601 aacccttgca taatccctcg gacgcgcagg aataggaccg attcactcgg gccctggcgc
      661 ctcgttcgtg tttaccccca gccatcgagc cggcccaaaa aactacgggc cgcacctctg
      721 gggggcgggg tttctcgaac cggagtcggg ctcacggatc gacgaacagg ttctcgaagg
      781 ggtcgagtgc ctcgggatcg ggattctctg gagctccgag tggcggaagc gcgcgtgggg
      841 gtgggagcgg gtccccccct gcccgaaagt ggaagtcctc gatctccgcg agcccatatg
      901 cgagggcctc ggggccgata cgttcgcgct tggccatgtg ctcgagaagc cgtcgcattc
      961 tgctcttcat ggagcgcgtc agtgtgcggc gctcgtagga gacgtgatag cgcttgggcg
     1021 acggcagcat gcacgcgaga aacgccgact cggcggggct cagctcgctt ggctctcttc
     1081 cgaagtaata cacggcagca tgcctcagtc cgtaaacgct cggaccgtac tcgatcacgt
     1141 tgaggtagag ctcgagtatc tggtctttgc tgagtgcatt ctcgagccac caagtgagga
     1201 tgacctcctg gaccttgcgc gcgagtgtct tttcccgttg caggtagagg ttcttggcta
     1261 gctgcatcga aatggtgctg gcgccgacga catagcggcc ctcctgaaga ttgcgcacca
     1321 aggcatcgcg aatcgcccac ggcgcaaatc cgccatgttc gtaaaaggct ccatcctcgt
     1381 gactgattac ggccggaacc acgaacgggc tgatgtccgc aaatgcgacc cagtactccg
     1441 ttcccgggcc ggtgcgcatc tcgaagaccg tctcgtcggg ctcgatggcg cgatgacgaa
     1501 atggcccttg aaaccgttcg acccgaaccc agctcggcgc acgctcgaac tcgcaaagat
     1561 tgcgaacgcg aatcgacaag tccgtctctt cgagcgttct cgagtcgagg gaaacatgtg
     1621 cgatgccgct ccaattccca gaccactcga actgagaaag cgagtcgagc acgtccgcgg
     1681 gtatggcgcc gacgacgtcg ttgcatcggg ttggcggaag cttggcagtg agatcgacgc
     1741 gatagtggtc cggagtgcgc tcgagctcgc catcgaaaag catctgagcc tcacccattc
     1801 gaacacggcc acgctcgatt tgaagccgtc gctcatccgg gtgccaaacg cctttccccg
     1861 agacatcgag attgacgtgc tccacaggct cggaagcgat gcgttcagag gcgagcgcca
     1921 actcgcgcaa ctcgagtctt ccttcgatgc gaacctgctg gggcgagctc gcggcgagct
     1981 cgatctcggc gctgaccgtg ccgacctccg tgttgtagag ggggaggtcc ggtgcgaaag
     2041 gagcgatgag agccagggag atgccacgga tgcgcaccat cccgtcggcg cgcgcctcgg
     2101 tgggcatcac gcgaagatcg acgtccaaag tgcccttgtt cgacgtctga ccatcgacgg
     2161 tgatccggaa ccagccatcg gcttccccac gcagaccgag ctcgaaatcc cgaagg
//
LOCUS       DASRZF010000264        21592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2262364, whole genome shotgun sequence.
ACCESSION   DASRZF010000264 DASRZF010000000
VERSION     DASRZF010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 21592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21592
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2262364"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1515)
                     /locus_tag="VFG22_11620"
     CDS             complement(<1..1515)
                     /locus_tag="VFG22_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416936.1"
                     /translation="MGWTTKIIKLCLAVTVLLAGCKVDGEDVQEWKATVKGPARLVAV
                     IGSDRYANELRTEAALAIVEMDRNDVDGLAILKHALNDLRAEDPAASERIVGGMVPRL
                     AALLAEAPGQIETKLDPIEVRAKDAAYMVIPYAPEESKDELIRAVVGWYSADFEGRSL
                     AGDYSAEQVTRAFGADAAGMLVDALDEKMPPQAMIKIAEIIGEDGGEETRKRAGERLV
                     EIEREMEKSAFVKWLATEIRGSLKASGEPIDEARVNVLALYNRENFINQGVLPAMRYL
                     GDQEEVAARLLAIADTTPGASDSKAWEERLDARRATALKALEGHVTRAHLNRLLSIAL
                     DSKNPLEVRDYAFDRVGDIRSRDAIPRLWPLLERVGCSAASCSKDDKLGKRLRWRAGE
                     LILSLGGPSTIEPFSQRLPAAPGIQYEPEELEGYATRMSQMTPPPTSTVLVLLNSQQW
                     WNRVVALRYLERRGGEADVPAMQRLKSDGETVLGQGWSELNPPVKTVGEVADAAI"
     gene            1663..4020
                     /locus_tag="VFG22_11625"
     CDS             1663..4020
                     /locus_tag="VFG22_11625"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HET6416937.1"
                     /translation="MSAHETTSSGDLLAPEGRRQDVLAILTLTATVLLTLALVSYDAR
                     NGGGNLVGPVGHRFARALMLAFGLVAWFLPLEGGLLTYRLFRRRSAMLGLATMASTLV
                     LVVIGASLAHLLIEPQALWGASPGGAVGELLGEVLRSLLGFYGTVIIGFAILATAVIL
                     RTPWTLEEVAQGFARLGHFVLAKIRSVFVALREAWAEAREIHEEEIRVSQPRIMGVHF
                     DDELEDEDEEPAPPLLVPAPSPRIPRSQANPAQSPTIVAPKETKQAPLAISMRSGIDH
                     KDFRLPPTSLLDEPDSSVIHVDAEALRENAELLTEKLAAYGVRGRVDEIHPGPVVTMY
                     EFEPQSGTKVSKIAGLADDLAMALAAQKVRIVAPIAGKARVGFELPNEIRQMVHLREI
                     IEDRRWEKMRGALPMALGKDIGGQPVYVDLARMPHLLVAGATGSGKSVALNVMLTSIL
                     AKKTPEEVRMLMIDPKVVELQVFDGIPHMLLPVVTDMKKAALALRWAVDEMERRYQLF
                     ADAGARNIDTYNRRVDRVLRGDLSVEKFMPKRKGKVSAQDVNGQEVLLDPSDGEAADA
                     EQFQPEKLPSVVVVVDEFADLMMVAAKDVEACIARLAQKARASGIHVILATQRPSVDV
                     ITGMIKANFPARVAFKVSQRQDSMTILGRSGAEHLLGMGDMLMIPPGASDLQRVHAAY
                     VSEDEVKAVCDFLREQGDPEYQEEILKPREDEGFDGESDDPLYDKAVSVVANAGYCSI
                     SHVQRQLSVGYNKAAKLVERMEQEGVVGPPSSKAGGRREVLVGPM"
     gene            4668..6080
                     /gene="bioA"
                     /locus_tag="VFG22_11630"
     CDS             4668..6080
                     /gene="bioA"
                     /locus_tag="VFG22_11630"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: protein motif:HMM:TIGR00508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HET6416938.1"
                     /translation="MGPNREHARREPFSGDTVVQHSGWHWRVRSRRRRVLWMSSDDEL
                     LALDRAHVWRPYTSSEDHERRSLFVVDRAEGPWLIAPDGRRILDASGSWWCNNLGHGH
                     PRLREAIRRQSERLMHCTFAAATHEPAVRLAAELVEVAPPGLNRVFYSDNGSTSVEVA
                     LKIAFQYWQQNGRPARKRFLALPGAYHGDTLGAMSAGDVDEFTRVFRPLLFGVERPPH
                     PIGGDWHPVFEHLIELLRRQTDQIAAVVVEPMVQGAAGMRMYPAELLRALHEATQQAD
                     TFLIADEVFTGFGRTGPMWGSGHAEITPDLLCTAKGLSGGMLPFAATLATDRIYDGFR
                     GDKTRALMHGHTFYGNPLGAAVAREVLAIYREEQVLEAAEAKARRLADRIHGMIEIAG
                     VAAPRALGMCAAFEVGAAGYMGTVGWRISDQALELGAHLRPLGNTVYVVPPLNIGDAD
                     LDQLLDIVRESTERVLRSAQ"
     gene            6077..7621
                     /gene="lnt"
                     /locus_tag="VFG22_11635"
     CDS             6077..7621
                     /gene="lnt"
                     /locus_tag="VFG22_11635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HET6416939.1"
                     /translation="MTEIEPSAPRRHPRYQAYGLAVLGGVLYFLGWAGFGFWPLAFLC
                     LVPLWAALELGLERSWRHSLMVGWSYGTVMCAGGYHWLVEFLDVFSGYGLAASALFWF
                     LFSVYLGCNFAIYGLLYRALRRRGWSTSTIAVPILLLIEWLYPSLFPTYLANSIQQRT
                     IMVQIAELGGPLLVTALMALVNLSFYEASRWARRDRSMPKLIWWLTAMSLFITTIYGA
                     VRIRQVEAEMKAAPALRLGIVQVNMGIFEKRTEAVEGHRRHLEQSHQLEDMEDLDLIV
                     WPESAYIGGLPRELPVRADRVRRDLKTPILFGGVSSEYLEGERKIYNTVFLIDEEGLI
                     QSTYDKTYLLMFGEYLPFGDLIPILYELSPNSGRFTPGSHVRPLTLGPWRISTPVCYE
                     DVLARFTRKMVREADPHVLINLTNDAWFGDTQEPWIHLVLSQFRAIEHRRYMVRATNS
                     GISAVIDPLGRIVAQTGVLTRENLRYDVEMLEGKTLYGRFGDWPGWIGLGAILWMLIR
                     RRGAER"
     gene            complement(7593..9317)
                     /locus_tag="VFG22_11640"
     CDS             complement(7593..9317)
                     /locus_tag="VFG22_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HET6416940.1"
                     /translation="MNLWFFLAMLGLVGCDFSSPETQTIVNSCASASDCSAGTCGDGI
                     CIDDSRASLEVAVEVLRGSSDPERVIPASWAFAAESVSSGTVRDLDLPATREVRGLVR
                     WADARVPATLSFVPRMQPGVASIAPVEVDVETFRDEASADGEQVHDFSVLLVAGESYD
                     LVVTPSNDLVLNPTDGAAPAIRSLPPLYLEVQVESGATTEPMRFDIAFPTNLSTACNA
                     LQNTSCTFDGQVLSVDADLEMEEPEAGLQVRAIDETSGRVVSSIAETDELGHFEIRIG
                     ETTSSYLIRITSSVGRDPFPSVSVNPTLAFPNNPLQNRIYVPRLDPIQFTGRVRDRDE
                     TPVSNATVRFLSTGIFAGTALGLDGSFSASATTDGEGRFGIPLLPGFYAITVTPPDDG
                     KSSWGVLTAEALVGEDVTTTEALIVPEQIGFRGWVATFLDEPAAGVTILARARPSAEA
                     GLVNRSQETVTNELGAFAMTLDTGLYDVHVKVSAETGFAWFVEPELLLSADDGERTRR
                     YLLEPPIPLQGLVRTSDGASVANAVVRAHILRYGEDGTTRSIQVAETVSGEDGTYRLL
                     IAPRLGAE"
     gene            complement(9314..9838)
                     /locus_tag="VFG22_11645"
     CDS             complement(9314..9838)
                     /locus_tag="VFG22_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416941.1"
                     /translation="MLYFASSITLVAAGASSGCIATDTIEFVPDQNFPPSIVSQALAE
                     FPLNEIGAIDLDEPVESPEMPLQVDIRDPNFDQTLDYRLFLDAPEPPAAEKPIQEGVI
                     DPTGFLDRPRTFSISYDELAAGQCHKIELLVAGMFASNTEQRRPVEEGDIDAVTWWVR
                     VTDAGNPVITQDCR"
     gene            complement(9944..11767)
                     /locus_tag="VFG22_11650"
     CDS             complement(9944..11767)
                     /locus_tag="VFG22_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET6416942.1"
                     /translation="MPSLKWMVPQGRPRVFGIYKRVTSIGRAGANDVSIDSAALEAHH
                     AQLVFDGRDFSVASIDSGAALLVNGKKKKKSKIFHNDKLTLGDIDLVFSLYDESANRD
                     AGPEQESSHTSEIAGMMKLSDFNRRLLEIRAIPDQIEALLDAVIEVVHANKGFVILLR
                     DGDPEIAAARNVDRETIPDAVTQLSDSILRRCIDTRQPLIVSDAVNDTMFNSSASVLN
                     LQLSSVMVAPLIAQGQLLGLIYVGNDNVVNLFEQSSLEVLTVFAGQASLIMQNAILLN
                     QLTTDRDRFAGELEAQRFGDIIGTCPSLQQVFRRVEKVAAADISVLITGETGTGKELF
                     ARELHRRSNRSEGPFIVVNCGAIPENLMESELFGHVRGAFTGAVSTRQGSFQAADGGT
                     LFLDEIGEMPLALQVKLLRALQERVVVKVGDTKPQSVDIRVLAATNRDLEVAIKEGAF
                     REDLYYRLNVVNLQLPPLRERGDDVSILAKFLLKKYTAEFGGAVKGFTPKALEAMHHY
                     DWPGNVRQLENRIKKAIVLADKTLIGPDDLDLGDDQQRAVIPLTEAREDFTRQYILEV
                     LERNGGNRTRTARDLGVDPRTVFRYLEREPGAPEPGGAVEG"
     gene            complement(11821..12264)
                     /locus_tag="VFG22_11655"
     CDS             complement(11821..12264)
                     /locus_tag="VFG22_11655"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET6416943.1"
                     /translation="MNTRIRDVLSSKGEGVYVVGPLATVIDAVNEMNDHHVGSVLVCE
                     DGYPVGIFSERDVLVRVVAAQRDPRRTLVREVMTTRLHTASPDDTLAEVMRLMTDRRC
                     RHVPVMEGDHLLGLVSIGDLTKASQSNLRHEVRQLSSYIGGPYLT"
     gene            12414..13127
                     /locus_tag="VFG22_11660"
     CDS             12414..13127
                     /locus_tag="VFG22_11660"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6416944.1"
                     /translation="MGHAKSQENRRSSHRVDLDGRAVLFQRGGRVGQYTLQNISAGGA
                     MVNGDRELRPGHLVHLLIDLESGEQTMSLTGSVRRTRNGSDGVGLAISFPILSADQED
                     AIHNAVLRSLLRRDARTPQLPILVFEPRRRVREEIESEIRSFGLCARGVDALADAVRE
                     LEGEETQYAALVIHSAAHDVRAMEVVEFFTRREELRTIILPDPDGALSEQAERLARLP
                     EVRVPRIWSRAELRDALGH"
     gene            complement(13124..14398)
                     /locus_tag="VFG22_11665"
     CDS             complement(13124..14398)
                     /locus_tag="VFG22_11665"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET6416945.1"
                     /translation="MKIFVESSDVLPLVDLEVAFPVGSLQDPAGLEGLAQLTCHLMRR
                     GPRGISQERFEDRLASLGARLSVQVSMRTTRIRATVLRRNVEPLFELVADLIWQPALR
                     AGDFAKLKRQAQAALLARLDDDQTLGALCFREALFGEHPYGRSLSGNAGTLSRIGVKQ
                     VEGFYKRHIEKGSFVVGLSGDLTEPEARSLVAAHFPTFRSRQHKTPAVPATRARRGRH
                     VVIVDKPERTQTQLFIGTLGARTRDRSLFPLIVSNTAFGGTFSGPLMQQVRAARGWSY
                     GAYSRLLHSNQRDAWYMATAPSVEYSADCAALQLDLLENWVETGIKKSELSFAKRYLI
                     NGHCFDRDTPSKRLDARLDVELLGIPRAYFEKHDDLVDRVTWQEATEATRARISTRDL
                     TIVVVASAADVVGPFERLPGVSSLEIVPFDRS"
     gene            complement(14395..15741)
                     /locus_tag="VFG22_11670"
     CDS             complement(14395..15741)
                     /locus_tag="VFG22_11670"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET6416946.1"
                     /translation="MGATEVLIERLNAGVRKRNARLEFVREQAFGDALRLRRYRLGNG
                     LTVLTLVDRSAPTVSYHTWFRVGSRHERPGKTGLAHLFEHLMFNETLNHPPGEFDRLM
                     ERAGAEANAATWTDWTYYYENAPRGSLPLLIELEADRMGNLVLRGPQVSSEKEVVVNE
                     RKMRVDDDAEGKALELLYRSAFRRHPYRWPTIGWMADIRAFTVQDCREFYRKHYAPSN
                     ATIVIAGDFNERTALSLVQKHYGGFSRAPVARQQTSSKEPLQHTERVFQLEAPTPTEK
                     ILIGYRAPAFSDPHTPALVIANEVLFGGQSSRLHRLFCIDKELALAVRGSISPFVDPG
                     LFEIWVFLREGKSKRNAIELLDRELERLGADGPTETEVEKAINQLELSFLHNMETAAG
                     KAEQIGFHETVLNDGAAVFDRLAAYREVTADEVKQAVRKYLRPSRRTRVEIVATAS"
     gene            15878..17332
                     /gene="gltX"
                     /locus_tag="VFG22_11675"
     CDS             15878..17332
                     /gene="gltX"
                     /locus_tag="VFG22_11675"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HET6416947.1"
                     /translation="MSEVRVRFAPSPTGYLHIGGVRTALYSWLWARQHKGTFILRIED
                     TDRERSTEESIQVVFDSMRWLGLDWDEGPEVGGDHGPYVQSERLDIYADYAQRLISSG
                     HAYRCYATKEEIADAREAHKKTGTKDGFRFRSPWRSQPEPAADPNAPHVIRFRAPEEG
                     TTSWVDAVKGKIDIPNATLQDSILLRPDGMPLYNFGCVVDDLTMGVTLVARGDDHVIN
                     TAPQILLYEALEAPVPTFAHMPMVLAPNGEKLSKRHAAVGVLEYRDMGYLPDAVLNYL
                     ARLGWSHGDQEIFTRAELIENFSWDRVGSTAGRYDAKKFLHVQAEHLRMLSDEDLARG
                     TVPFLQKIGIEVAEDDPLLLKTIPYVRPRAETFVDVAEAVEYFFRDVEFEEKGKRKFL
                     VPSSAEHLMRLAEVVEAVEPFAQGPLDEAVKAWLEANDLQMKVVAQPARVAMTGRTRS
                     PGLFEVMEVLGKERTLARLRFGAQIAAAPEAQSS"
     gene            17357..18907
                     /locus_tag="VFG22_11680"
     CDS             17357..18907
                     /locus_tag="VFG22_11680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6416948.1"
                     /translation="MELTVQTLVIVLYLVTLGVLALYGCHRSALLFLYLRHRGEQTLP
                     KARFEDDHLPFVTIQLPMYNELFVAERLIDAVARIEYPRERLQIQVLDDSTDETSIIA
                     VAKCKALRKQGIDVEYLHRTDRHGYKAGALAAGLERAKGELVLVFDADFVPGTEILGQ
                     LVHFFTDPRVGMVQARWAHLNPDRSLLTRCQAMLLDGHFVIEHAARNRSGRFFNFNGT
                     AGIWRKTAIREAGGWQHDTVTEDMDLSYRAQLVGWKFVYAPEVSVPAELPGEMNSFKG
                     QQYRWAKGSVQTARKLLGTILRAPVGAKVKSEAFFHLSNNLAYVFLLVLAALQLPNML
                     IRRKMEHPELLMLDVPLFLATCGSVAAFYVVAHHELYGGRWQAIKRLPAMMALGIGLS
                     INNGRAAIEGLFGRDVEFIRTPKRGTIDGGPSAGTSRYRGRWPWHNTIELLFGLYCLA
                     TLAIAISTRSWASVPFLLLFCAGFTYVGVLSLFEAFQRKPGHGDDSTRAEQEPSTHSS
                     PRPQSMLS"
     gene            complement(18829..19626)
                     /locus_tag="VFG22_11685"
     CDS             complement(18829..19626)
                     /locus_tag="VFG22_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416949.1"
                     /translation="MKRLGILIIALFGLVGCDRDSRYVCIADGPLSDRGCFLCRGDEC
                     DSVPAPERNICVAEPDCAVGEVCTTIGCVRACEDEYDCPLGTTCEENGLCLNPLEEDP
                     APQAFRCQFNFECGDSRICIDGECLITCLDSPCPDTQQCVAGACRPCTDDTCLTRCTE
                     DANCEEHEYCSSFQCIPDTRPEQFCPENECQPGRLCVRGQCRTPCETDEECARIDASI
                     RFCAPVEEQNLCVRSSEVLAECQLNIDCGLGEECVDGSCSARVESSP"
     gene            19714..20460
                     /locus_tag="VFG22_11690"
     CDS             19714..20460
                     /locus_tag="VFG22_11690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET6416950.1"
                     /translation="MSGHSKWSTIKRRKGAQDAKRGKLFTKVIKEITIAARSGGGDLE
                     GNARLRRAVDAARNVNMPADNVTRAIKKGTGELEGVQYEELLYEGVGPDGTLFICEVV
                     TDNRNRTIAEVRKLFDKNHGQISSSGAALWAFEQKGMIRIEKDAASEEKLFEAAVGGG
                     AENMELLEEQWVVTTARNDLDSVRDAIASAGIEVSEATLEYIPNNPKVVEGDEAMKLI
                     QLYEALEEHDDIQNVFSDFELSEQALAEMA"
     gene            20444..20953
                     /gene="ruvC"
                     /locus_tag="VFG22_11695"
     CDS             20444..20953
                     /gene="ruvC"
                     /locus_tag="VFG22_11695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HET6416951.1"
                     /translation="MQRWPERVMRVLGIDPGSRRTGWGVVQLEGTRLLHVGAGTIAVP
                     EKLALEKRLQLIHAGLEQVIAEHSPDAVAVEEIFFAKYANAALKLGHARGIALLVAAQ
                     SDLEVHEYPPAIVKRTVVGRGAADKVQVTRLITALLGLKAAPEEDAADALAVAITHIQ
                     ASRSKAIAR"
     gene            21079..>21592
                     /gene="ruvA"
                     /locus_tag="VFG22_11700"
     CDS             21079..>21592
                     /gene="ruvA"
                     /locus_tag="VFG22_11700"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HET6416952.1"
                     /translation="MIGRLSGTIEHREADGSVIVDVNGVGYEVFLPPGAAGRLGSKPG
                     EAVTLEIHTHVREDAMTLFGFATPEDRAAFRALLKVSSIGPKLALAIIGVMNASELQD
                     AIARQDKTAFKGITGVGKRTVERILVDLQDKLDFAPTGRTGVRLRAVPSTGSSIEDRV
                     VGALVQMGYKR"
BASE COUNT         4187 a         6657 c         6699 g         4049 t
ORIGIN      
        1 tatggctgcg tcagcaactt cccctaccgt tttcacgggc gggttgagct ctgaccatcc
       61 ctgtcccaaa actgtttctc catccgactt caggcgctgc atcgcgggca catccgcctc
      121 cccgcccctg cgttcaagat agcgcaatgc gaccacacga ttccaccact gttgcgaatt
      181 cagaagcacc aaaacggtag atgtaggtgg aggtgtcatt tggctcatcc gagtggcgta
      241 gccttcgagc tcctcgggct cgtactggat ccccggagct gccggtaatc gttgcgaaaa
      301 cggctcgatc gtagagggac ccccgagcga gaggatcaac tcgccggctc gccaacgaag
      361 ccgttttccg agcttatcgt ccttggagca ggaggcggcg ctgcagccca ctcgctcgag
      421 caatggccac aatcgcggga tcgcgtctcg actgcggatg tcgccgacgc ggtcgaaagc
      481 atagtcgcgg acctcgagtg ggtttttgga atcgagcgct atcgaaagca agcggttgag
      541 gtgcgctcgg gtcacatgcc cttcgagcgc cttcagcgca gtggcccggc gcgcgtcgag
      601 cctctcttcc caagccttgc tgtcgctcgc ccccggggtg gtgtccgcga tcgcgagcaa
      661 gcgtgcggcc acctcttcct ggtcgcccag gtaccgcatg gcaggaagga caccttggtt
      721 gatgaagttc tcgcgattgt agagagccaa aacgttgacg cgtgcctcgt caatcggctc
      781 accggatgct ttgagggacc cacggatctc agtggcgagc cacttcacga acgcggattt
      841 ctccatctca cgttcgatct cgaccaggcg ctcgcctgcg cgcttccgtg tctcctcacc
      901 gccgtcttcg ccgatgatct ccgcgatctt gatcatcgcc tgcggcggca tcttttcgtc
      961 gagagcatcg accagcatgc ccgccgcgtc tgctccaaat gcacgggtta cctgttcggc
     1021 gctgtagtcc cctgcaagac ttcgtccttc gaaatcggcg gagtaccagc cgaccaccgc
     1081 acgaatcaac tcgtctttcg actcctcggg cgcgtaggga atcaccatgt acgcggcgtc
     1141 cttcgcgcga acctcgatgg ggtcgagctt ggtctcgatc tggccggggg cctcggcgag
     1201 cagcgcggca agtcgtggca ccattccgcc gacgatccgc tcgctcgcgg ccggatcctc
     1261 tgcgcggaga tcgttcagcg cgtgcttgag aatagctaga ccgtcgacgt cgttgcggtc
     1321 catctcgacg attgcgagcg ccgcctcagt cctgagctca ttcgcatacc gatcgctacc
     1381 gatcacggcg acgaggcggg cggggccttt gaccgtcgcc ttccattcct ggacgtcctc
     1441 gccgtcgacc ttgcatccgg ccagcagcac agtgacggcc aagcaaagct tgatgatctt
     1501 cgtcgtccaa cccatccgac tcgcggaacc atcccccctc gggcatgcaa cccggcaaga
     1561 taatagagga ttccgccaag caatcaagaa cgtcgaagag ccgtgatttc ttgaccaacc
     1621 ccaggttcgc cgcatagcct ctgtgtgtag gaggtcgctt acttgagcgc acacgaaacc
     1681 acctcttcgg gcgatctttt ggcacccgaa ggtaggcggc aggacgtcct tgcgatcctg
     1741 accctcacgg caaccgtact tttgaccctt gcgctggttt cgtatgacgc ccgaaacggc
     1801 ggaggaaacc tggttgggcc ggtgggtcat cgattcgcgc gggccttgat gctggcgttc
     1861 gggctggtcg cctggttctt gccgctcgag gggggcttgc tcacctatcg cttgtttcgt
     1921 cgacgctctg cgatgctggg cctggcgacg atggcctcga ctctcgtcct ggtggtgatc
     1981 ggcgcttcac tggcccatct cttgatcgag ccgcaggccc tctggggcgc atcgcctggc
     2041 ggagcggtcg gcgagctgct cggcgaggtt ctccgctcgc tgctcggatt ctacggcacc
     2101 gtgatcatcg ggttcgcgat tctcgccact gcggtgatct tgcgaactcc gtggacgctc
     2161 gaagaagtgg cgcagggatt cgcgcggctg ggtcatttcg ttctggccaa gatccgaagc
     2221 gtcttcgttg cgcttcgcga agcatgggcc gaggcacgcg agatccacga ggaagagatc
     2281 cgcgtgagcc agcctcgcat catgggcgtt cacttcgacg atgagctcga agatgaagac
     2341 gaagaaccgg cgccgccgct gctggtgccc gctccgagtc cacggatccc gagatcacaa
     2401 gccaaccctg cgcagtcccc gacgatcgtc gcgcccaagg agaccaagca ggcgcccctt
     2461 gcgatctcga tgcgctcagg gatcgaccac aaggacttcc gccttcctcc gacgagctta
     2521 ctcgacgagc cggattcttc cgtcatccac gtcgatgcgg aggcgctccg cgaaaacgcc
     2581 gagctactca ccgagaagct cgcggcttac ggggtgcggg ggcgcgtcga cgagatccat
     2641 ccgggcccag tggtcaccat gtatgagttc gagccgcaaa gtggaaccaa ggtctccaag
     2701 atcgcaggcc tagcggacga cctcgccatg gccctggctg cgcagaaggt gcgcatcgtg
     2761 gctccgatcg caggaaaggc gcgcgttggc ttcgagctcc cgaacgagat ccgtcaaatg
     2821 gtgcatctcc gcgaaatcat cgaagatcgt cgctgggaaa agatgagagg cgcgctcccg
     2881 atggcgctcg gcaaagacat cggaggtcag ccggtctacg tagacctcgc gcgtatgccg
     2941 cacttgctcg tcgcgggcgc aacgggttcg ggcaagagcg tcgcgctcaa cgtgatgctg
     3001 acgtcgattc tagcgaagaa gaccccggaa gaagtccgca tgctgatgat cgacccaaag
     3061 gtcgtcgagc tacaggtctt cgacggtatc ccgcacatgc tgctccccgt cgtcaccgac
     3121 atgaagaagg ctgcgctggc gcttcgctgg gcagtcgacg agatggagcg gcgctaccag
     3181 ctcttcgccg atgctggtgc tcgcaacatc gacacttaca accggcgtgt cgatcgggtg
     3241 ctgcggggag acctcagcgt cgagaaattc atgccgaagc gcaagggcaa agtcagcgct
     3301 caagatgtca acggccagga ggttctactc gatccgagcg atggcgaggc ggcggacgca
     3361 gagcagtttc agcccgagaa gttgccctcg gtggtggtcg tcgtcgacga gttcgcagac
     3421 ctgatgatgg tggcagcgaa ggacgtcgaa gcatgcatcg ctcgtcttgc ccagaaggcc
     3481 cgtgcttccg gcattcatgt gatcctcgcc acccagcgcc cgagcgtcga tgtcatcact
     3541 ggcatgatca aagccaactt tccggcgcga gtcgccttca aggtttcgca gcgccaagac
     3601 tccatgacga ttctcggtcg atctggcgcc gagcaccttc tcggaatggg agacatgctc
     3661 atgatcccgc ccggagcgag cgacctccag cgcgtgcacg cggcatacgt aagcgaagac
     3721 gaagtgaagg ccgtctgcga cttcctccgg gagcaaggcg atccggaata ccaagaagag
     3781 atcctcaaac cgcgtgagga tgagggtttc gacggcgaaa gcgacgaccc actctacgac
     3841 aaagcggtct cggtcgttgc aaacgcaggg tactgctcga tcagccacgt gcagcgacag
     3901 ctgagcgtcg gctacaacaa ggccgcgaag ctcgtcgagc gaatggagca agaaggcgtc
     3961 gtcggacctc cgagctccaa agccggcgga cgaagagaag tcctcgtcgg accgatgtaa
     4021 tcggatcgcg gctcgcgacg gaccggcacg ggcgaacacg acgcgggcat tcagcgggcg
     4081 gggcgccttc atgacgtgca atcggagagc ggctcgcgac gcacgtgctc gcgattggga
     4141 cccgctcggc cgtctctcta cgactccata cgtctagcct cccgcccaat cgctgtgccg
     4201 cccatcccga gccgctctcc tctgtacacg ccaaccatgc acactggcac tgacgtctcc
     4261 gtacggaggg acgcggcgga acgcgcggca cagcgattgg gcgatcgggt cacggcggaa
     4321 cgcgacgcgg ggttccgtcg gacggctcgg cttgcaattg tggccggtgg gacttgaggc
     4381 cgggggccac gcaagcctcg cctcggagct cgcgccaatc gggggtggtt gcttcctgac
     4441 cgattgtcac tcgctacgcc gccgaaaccc cacatcccgt tccgccatgc ccctccatac
     4501 gtcagggtca gcgtcatcga caggttccaa ttggcaccgt cacggacaac gtcgctcacg
     4561 tggacgctct gtcgtaggtg gacgcggacg gaggaggacg gctcgcgatg ggcagcacag
     4621 cgattgggcg ggaggctaga cgtatggagt cgtagagaga cggccgagtg ggtcccaatc
     4681 gcgagcacgc ccgtcgcgag ccgttctccg gtgacaccgt ggtgcagcac agtggctggc
     4741 actggcgcgt tcgttcgcgc cggcgcagag ttctatggat gtcctctgac gacgagttgc
     4801 tcgcgctcga tcgggcgcac gtttggcgcc cgtatacgtc gagtgaggac catgaacggc
     4861 gctcgctctt cgtcgtggat cgggccgaag ggccctggtt gatcgctccg gatgggcgac
     4921 ggattctgga cgcgagcggc tcttggtggt gcaacaacct cggacacggg cacccccgac
     4981 ttcgagaggc gatccgacgt caatccgagc gactcatgca ctgcaccttc gcggcagcca
     5041 cgcacgagcc agcggtgaga ctcgcggccg agctggtcga ggtcgcccct ccgggcctga
     5101 accgggtgtt ctacagcgac aacggctcga cctcggtgga ggttgcgctg aagatcgcgt
     5161 ttcagtactg gcaacagaac ggaaggccgg cgcgaaagcg attccttgcg ctgccgggcg
     5221 cctatcacgg tgacaccctg ggcgcgatga gcgcgggcga cgtcgatgag ttcactcgtg
     5281 tcttccgacc actccttttc ggcgttgaac gcccaccgca cccgatcggc ggtgactggc
     5341 atcccgtctt cgagcatttg atcgagctcc tccgtagaca gacggatcag atcgccgccg
     5401 tggtcgtgga accgatggtg cagggggccg cgggcatgcg aatgtaccca gccgagctcc
     5461 tccgtgcgtt gcacgaggcc acccagcagg ccgatacctt tctcatcgcc gatgaggtct
     5521 tcacgggttt cgggaggacc ggaccgatgt ggggttctgg gcacgccgag atcacgcccg
     5581 atctcctctg cacggccaag ggtctatccg gaggtatgct tcccttcgcc gccacgctgg
     5641 cgacagatcg catctacgat ggattccgag gcgacaagac ccgtgcgctc atgcatgggc
     5701 acactttcta cggcaatccc ctgggcgccg cggtcgctcg agaggtgctg gccatctatc
     5761 gcgaagagca ggttctcgaa gcagcagagg ccaaagccag gcgcctcgcg gaccgaattc
     5821 atggaatgat cgagatcgcc ggcgttgccg caccgagggc gctcggcatg tgcgcggcct
     5881 tcgaggtcgg cgcagctgga tacatgggca cggtcggttg gagaatctcc gatcaagcgc
     5941 tcgagctcgg cgcgcatctt cgtcccctcg gcaacaccgt ctacgtcgtc cccccgctca
     6001 acatcggcga tgcggacctc gaccagttgc tcgacatcgt gcgcgaatct accgaacgcg
     6061 tgctacgaag cgcgcagtga ccgagatcga gcccagcgcc ccaagacgac acccgcgata
     6121 tcaggcgtac ggtctcgcag ttctcggtgg cgttctctat ttcctcggct gggcgggctt
     6181 tggattttgg ccgctcgcct tcctttgcct cgtcccactc tgggccgcac tcgagctcgg
     6241 actggagcgc agctggcgcc acagcctgat ggtcggctgg tcgtacggga ccgtcatgtg
     6301 tgcgggcggc taccattggc tcgtcgagtt cctggacgtg ttctcgggtt acgggctcgc
     6361 ggctagcgcg ctcttctggt ttctgttctc ggtttatctc gggtgcaact tcgccatcta
     6421 tggattgctc taccgcgcgc tgaggcgccg cggctggagc acgagcacca ttgcggtccc
     6481 aatcctgctt ctgatcgagt ggctctatcc ctcgctcttt ccgacctatt tggcgaacag
     6541 catccagcag cggaccatca tggttcagat cgccgaactt ggggggccac tgctcgtcac
     6601 cgcgctgatg gccctagtca acctgtcgtt ctacgaggct tcgcgctggg ctcggcgcga
     6661 ccggagcatg cccaagctga tctggtggct cactgcgatg tcgctcttca tcacgacgat
     6721 ctacggcgcc gtccgaatac gtcaggtcga agccgagatg aaggcagcac ctgccctccg
     6781 gctgggaatc gtgcaggtga acatggggat cttcgaaaag cgcaccgaag cggtcgaagg
     6841 gcatcggcgc cacctcgagc aaagccatca gctcgaggat atggaggacc tcgacctcat
     6901 cgtttggcca gaatccgcgt acatcggggg actaccccgg gagcttcccg tgcgcgcgga
     6961 ccgcgttcgc agagacttga agacaccgat tctctttggt ggcgtctcga gcgagtacct
     7021 cgaaggtgaa cgcaagatct acaacaccgt atttctcatc gatgaggaag gcctgatcca
     7081 gtcgacatac gacaagacct acctgctgat gttcggggag tacctccctt tcggagacct
     7141 catcccgatt ctctacgagc tctctcccaa cagtggtcgt ttcacgccgg gctcgcacgt
     7201 tcgcccactg accctgggac cttggcggat ctcgacgccg gtttgctacg aggacgtcct
     7261 tgcgcggttc acgaggaaga tggtgcggga agctgatccc cacgtgctga tcaacctcac
     7321 caacgatgcc tggttcggcg acacccagga gccttggatt cacctcgtct tgtcgcagtt
     7381 ccgggccatc gagcataggc gctacatggt gcgggcaacc aacagcggga tcagcgccgt
     7441 catcgatccg ctgggccgca tcgtggccca gaccggagtg ctcacgcgcg aaaaccttcg
     7501 ctacgacgtg gagatgctcg agggcaagac gctctacgga cgcttcggcg actggcccgg
     7561 ctggatcggt ctcggcgcga ttctctggat gctcattcgg cgccgaggcg cggagcgatg
     7621 agcaagcgat aggttccgtc ctcgccggaa acggtttcgg caacctggat cgagcgagtt
     7681 gtaccgtcct cgccgtacct gaggatgtgc gcgcgaacga ccgcgttcgc cacgctcgcc
     7741 ccgtcgctgg tgcgcaccag gccttgaagt gggattggcg gctcgagaag gtatcgacgc
     7801 gtgcgctcgc cgtcatcggc actgagcagc agctccggct cgacgaacca agcaaagccc
     7861 gtttccgccg agaccttgac gtgcacatcg tagagacccg tatcgagagt catggcaaag
     7921 gcgccaagct cgttggtcac ggtctcctgc gatcgattga ccaagcctgc ctcggcgctc
     7981 ggacgagcgc gcgcgagaat ggtgacgccc gccgcgggct cgtcgaggaa tgtcgctacc
     8041 cagcctcgaa atccaatctg ctcgggaacg atgagggctt cggtcgtggt gacgtcctca
     8101 cccacgagcg cctccgcggt gagcacaccc caggagctct tgccgtcgtc cggtggcgtc
     8161 accgtgatcg cgtagaagcc gggaagaagc ggaatgccaa aacggccctc accatcggtc
     8221 gttgcagacg cgctgaatga gccatcgagg ccaagcgccg tccccgcaaa aatacccgtc
     8281 gagaggaatc gcacggtcgc gttcgacacg ggggtttcgt cgcgatcgcg cacccggccc
     8341 gtgaactgga tggggtcgag gcgtggaacg tagatgcggt tctggagggg attgtttggg
     8401 aacgccaggg tcggattgac ggacaccgat gggaagggat cccgcccaac gctcgaggtg
     8461 atgcgaatca gatagctcga cgtcgtctcg ccgatacgaa tctcgaagtg accgagctca
     8521 tccgtctctg cgatggagga tacgactcgg ccgctggttt cgtcgatcgc gcgaacctgc
     8581 aagcccgcct cgggctcttc catctccaag tccgcatcga cgctgagcac ctggccatcg
     8641 aaggtgcaac tggtgttctg aagcgcgttg caggccgtgg agaggttcgt cgggaacgcg
     8701 atgtcgaatc gcattggctc agtggtcgca ccactctcga cttggacctc gaggtagagc
     8761 ggcggcaagc tgcgaatcgc gggcgccgca ccatccgtgg gattcaagac caggtcgttg
     8821 ctcggtgtca cgaccaggtc gtagctttcg ccagcgacca gcaggacgct gaagtcgtgg
     8881 acctgctccc cgtccgcgct ggcctcgtcg cgaaaggtct cgacgtctac ctcgacaggc
     8941 gcgatcgaag cgaccccggg ttgcattcgc gggacgaagc tgagcgtcgc aggaactcgc
     9001 gcgtcggccc agcgaaccag gccgcgaacc tcgcgcgtcg caggaagatc gaggtcgcga
     9061 accgtcccgc tggagaccga ttcagcggca aacgcccagc tcgccgggat cacgcgctcg
     9121 ggatcgctcg aacctcgaag aacctcgacc gcaacttcga gggacgcgcg cgaatcatcg
     9181 atgcagatcc cgtcgccgca ggtcccggcc gaacaatccg aggcgctcgc gcaggagttt
     9241 acgatggttt gagtctctgg cgagctgaag tcgcacccga cgagaccgag cattgcgaga
     9301 aaaaaccaga gattcatcgg caatcctgag tgatgacggg attgccggcg tcagtcaccc
     9361 tgacccacca ggtgaccgca tcgatgtcgc cctcttcaac cgggcgacgc tgttcggtgt
     9421 tgctcgcaaa catcccagcc acgagcagct caatcttgtg gcattggcct gccgcgagct
     9481 cgtcataaga aatgctgaac gtgcgcgggc ggtccaggaa gcctgtggga tcgatgacgc
     9541 cctcttggat tggcttctcg gcggccggcg gttcgggcgc gtcgaggaag agccggtagt
     9601 cgagagtctg gtcgaagttc ggatcccgga tgtccacttg gagcggcatc tcgggcgact
     9661 ccacaggctc gtcgaggtcg atggcgccga tctcgttcag cggaaactcg gcaagagctt
     9721 ggcttacgat cgacggcgga aagttctgat ccggaacgaa ttcgatcgtg tcggtggcaa
     9781 tacagccact gctcgcgcca gcggcgacca gcgtgatcga tgaagcaaag taaagcatcc
     9841 ggaagaattt agaagcaaag gccatgccgc gatctcgaaa ccgaaaaacc tgagatttcc
     9901 gcaactatcc cacggattcc caggcttaac tatgacatta atgtcagccc tcgaccgctc
     9961 caccgggctc gggagcgccc ggttcgcgct cgaggtatcg gaaaaccgtt cgcggatcga
    10021 cgcccaaatc tcgcgcagtg cgcgtccggt tgccgccatt ccgctctagg acctctagaa
    10081 tgtactgacg ggtgaaatcc tcgcgcgcct ccgtgagtgg gatcaccgcc cgctgctgat
    10141 catcgccgag gtcgaggtca tcgggcccga tgagcgtctt gtccgcaagc acgatcgcct
    10201 tcttgatgcg gttctccaac tggcgaacgt tgcccggcca atcgtagtgg tgcatcgctt
    10261 cgagcgcctt gggcgtgaac cccttgacgg cgccgccgaa ctcggccgtg tacttcttga
    10321 gcaggaactt cgccaggatc gaaacgtcgt caccacgctc gcgaagcggc ggcaactgaa
    10381 ggttcacgac gttcaagcga taatacagat cctcgcgaaa cgccccctct ttgatggcaa
    10441 cctcgaggtc gcggttcgtc gcggccagaa cacgaatgtc gaccgactgt ggcttggtat
    10501 cgcccacctt cactaccacg cgttcctgaa gcgcacgcag aagctttacc tgaagcgcaa
    10561 gcggcatctc gccgatctcg tcgagaaaca aggtgccccc atcggccgct tggaagctgc
    10621 cctggcgagt cgacacagcc ccggtgaacg cccctcgcac atgaccgaag agctccgact
    10681 ccataaggtt ctctggaatc gcgccacagt tcactacgat gaatggaccc tctgagcggt
    10741 tcgaacggcg gtgcagctct cgcgcgaaga gctccttgcc ggtgccggtt tcgccagtga
    10801 tcagcacact gatgtcggcc gcagccacct tttccacgcg ccgaaagacc tgctgcaagc
    10861 tcggacaggt gccgatgata tccccgaagc gctgcgcctc gagctctcca gcgaagcgat
    10921 cgcgatcggt cgtgagctga ttgaggagaa tagcgttctg catgatcagc gacgcctgcc
    10981 ctgcgaacac cgtcagcact tcgaggctcg attgctcgaa gaggttcacg acgttgtcgt
    11041 tcccgacgta gatcagtccg aggagctgac cctgcgcgat gagaggcgcg accatgacgg
    11101 agctgagctg caaattgagc acgctggcac tcgaattgaa catcgtgtcg ttcaccgcat
    11161 cgcttacgat caaaggctga cgagtgtcga tgcagcggcg aagaatgctg tcggaaagct
    11221 gcgtcactgc gtccggaatg gtttcgcggt cgacgtttcg agcggccgcg atctcgggat
    11281 cgccatctcg aagcaagatt acgaagccct tgttcgcgtg cacgacttcg atcacggcgt
    11341 cgagcagtgc ttcgatctga tccggaatcg ccctaatttc caagagccga cgattgaagt
    11401 cgctcaactt catcataccc gcgatctcgg aggtgtgaga cgactcttgc tcggggcctg
    11461 cgtcgcgatt cgcgctttcg tcgtaaagcg aaaagacgag atcgatgtcg cccagcgtca
    11521 gcttgtcgtt gtggaagatt ttagatttct tcttcttctt gccgttgact agaagagccg
    11581 cgccggaatc gatagaagcg acggaaaagt cgcggccgtc gaacaccagc tgggcatgat
    11641 gagcctcgag tgcggctgaa tcgatcgaca catcgttggc cccggcccgc ccaatcgagg
    11701 tcacgcgctt gtaaatccca aacacgcggg gccggccctg aggcaccatc catttgaggc
    11761 tcggcatagg tgagggactc taacagacca gcgccacgag cggcaggatc cgtgccaacg
    11821 tcaggtcagg taagggccgc cgatgtagct cgaaagctgt cgcacctcgt gtcgaagatt
    11881 ggattggctg gctttggtga ggtcgccgat cgagacgagg cccagcaggt gatcgccctc
    11941 catgaccgga acatgtcgac acctacggtc ggtcatcagg cgcatcactt cggccagagt
    12001 gtcatcgggt gatgccgtat gaagacgggt ggtcatcact tctcgcacca acgtccggcg
    12061 cggatcccgc tgcgcggcca cgactcgaac caaaacgtcg cgttccgaaa agatcccgac
    12121 gggatagccg tcttcgcaaa ccagcacgga tcccacgtgg tggtcgttca tctcgttcac
    12181 ggcgtcgatc accgtcgcga gcggacccac cacatatact ccctcgcctt tgcttgaaag
    12241 aacgtcgcgt attcgagtgt tcatcatcag cacacctctt gcttcggcga ttatacaatc
    12301 atccaacatt ttgcgaagcc cttatgagcg gatcgggcct cctgcacgac cgaacgggct
    12361 atcacccctt gcggactagc ttttcccgcg ccggctcggc actctgatgg taaatggggc
    12421 acgccaagtc acaagagaat cgacgctcga gccacagggt ggatctcgat ggccgggcgg
    12481 tactgtttca gagaggcggc cgcgtcgggc aatacacgct ccagaatatc tctgcgggcg
    12541 gcgctatggt gaacggagat cgggagctgc gcccagggca cctggtccac ctcttgatcg
    12601 acctcgaatc gggtgagcaa accatgagcc tcacggggtc ggtacggcgc actcgaaacg
    12661 ggagcgacgg cgtggggcta gcgatctcgt ttccgatact ctccgcggac caggaagatg
    12721 cgatccacaa cgcggtgctt cgatcgctgt tgcgacggga tgcgcggaca ccccagcttc
    12781 cgatactggt attcgaaccc cgccgccgtg tacgcgagga gatcgagtcg gagatcagaa
    12841 gctttggcct ctgcgctcgc ggagtcgatg cactggcgga cgcggtccgc gagctcgaag
    12901 gcgaagagac ccaatacgcg gcgcttgtga ttcactcggc agcgcacgac gtgcgtgcga
    12961 tggaagtcgt ggagttcttc acgcggcgcg aggagcttcg cacgatcatc ctgccggatc
    13021 cggacggcgc cctgagcgag caggccgaac gcctggctcg tcttccagag gtacgagtgc
    13081 cacggatctg gagccgggcc gagctacggg acgcgctcgg ccactagctt cgatcgaaag
    13141 gcacgatctc caacgagctc acgcccggga gccgctcgaa tggtcccacc acgtcggctg
    13201 cgcttgcaac gacgacgatc gtcaggtcgc gggtcgaaat ccgagcccgg gtcgcctcgg
    13261 tggcctcctg ccaggttacc cggtccacca aatcatcatg cttctcgaag taagctcgag
    13321 gaattcccag aagctccacg tcgaggcgag cgtcgaggcg cttggatgga gtgtcgcgat
    13381 cgaagcaatg accgttgatc aggtatcgct ttgcaaagct cagctccgac ttcttgattc
    13441 cggtttcaac ccagttctcg agcaagtcta gctgcagggc ggcgcaatcg gcagagtact
    13501 cgacggacgg tgcggtagcc atgtaccagg cgtcgcgttg gttcgaatgt agcaagcggc
    13561 tgtaggcgcc atagctccag ccgcgagctg cacgaacttg ttgcatcagt ggaccactga
    13621 aagtgccgcc aaatgcggta ttcgagacga tcagcggaaa tagactacgg tctcgcgtcc
    13681 gcgcgccgag ggttccgatg aataattgtg tttgagttcg ttcgggcttg tcgacgatca
    13741 ccacgtgccg gccacgacgt gcgcgcgtcg caggcacggc tggagtcttg tgctggcggg
    13801 agcgaaacgt cgggaagtgg gcggcaacca gcgaacgggc ttcgggctcg gtcaagtccc
    13861 cagacaagcc tacgacgaac gacccctttt cgatgtgacg cttgtaaaac ccttctactt
    13921 gcttcacccc gatccgtgac agggtccctg cgttgccgct gagcgagcgg ccatacggat
    13981 gctctccaaa gagcgcttcg cggaaacaca gcgcccccaa ggtttgatcg tcgtcgaggc
    14041 gggccaagag cgcggcctga gcctggcgtt tgagctttgc aaagtcaccc gcgcgcagag
    14101 ccggctgcca gatcaagtcg gccacgagct cgaaaagtgg ttcgacattg cggcgcaaca
    14161 cggtcgcgcg aatgcgagtc gttcgcatcg atacttgcac ggaaagacgc gcaccgaggc
    14221 tcgccaagcg atcctcgaat cgctcctgcg agatgcctcg gggcccgcgg cgcatcagat
    14281 ggcaggtgag ttgtgcgagg ccctctaggc ccgccggatc ttgcaaacta ccaaccggaa
    14341 aagcgacctc gagatccacc aagggcagca catcgctcga ctcgacgaag atcttcatga
    14401 tgccgtggcc acgatctcga cccgggttcg ccgcgaaggt cgaaggtact tcctcacggc
    14461 ctgcttcacc tcatccgccg tgacctcgcg ataggccgcg agacgatcga atacggctgc
    14521 gccatcgttg agcactgtct cgtgaaaacc gatttgctcg gcttttccgg cagccgtctc
    14581 catgttgtgc aagaaggaca gctccagttg attgatcgct ttttcgacct cggtctcagt
    14641 cggcccatcg gcgccaagac gttcgagctc ccgatcgagc agctcgatcg cattgcgctt
    14701 ggactttcct tcgcgcagga atacccagat ctcgaaaagc cctggatcga cgaatggcga
    14761 aatcgagcct ctcacagcga gcgcaagctc tttgtcgatg cagaagagac ggtggagacg
    14821 tgagctctgc ccaccgaaca agacctcgtt ggcgatgacc agggcaggcg tgtgcgggtc
    14881 ggaaaaagcc ggtgcacgat agccgatcag gatcttctcg gtcggtgtcg gcgcctcgag
    14941 ctggaacacc cgttcggtat gctgcaaggg ctctttcgaa gacgtttgct ggcgcgccac
    15001 aggagcgcgc gaaaagccgc catagtgctt ctgaactaga gaaagagccg tccgctcgtt
    15061 gaagtcaccc gcgatcacga tcgtggcgtt gctcggtgca tagtgctttc ggtagaattc
    15121 gcggcagtcc tggacggtga acgcgcggat gtctgccatc cagccgatcg tgggccatcg
    15181 atacgggtgt cggcgaaacg cgctacggta cagaagctct aaggccttgc cttcggcgtc
    15241 gtcatcgacg cgcatttttc gctcgttgac gactacctct ttctcggacg agacctgcgg
    15301 tccgcggagg accaggttgc ccatccggtc agcctcgagc tcgatcaaga gcggcaagga
    15361 accacgaggc gcgttctcgt agtagtacgt ccagtccgtc caagtggccg cgttggcctc
    15421 ggcgccagcc cgttccatca agcgatcgaa ctcgccgggc gggtggttga gggtctcgtt
    15481 gaacatcagg tgctcgaaca agtgtgcgag gccggtcttg cctgggcgct cgtgccgaga
    15541 gcccacgcga aaccaggtat gatagctgac cgttggcgcc gagcggtcga cgagcgtgag
    15601 caccgtcaac ccgttaccca agcggtagcg ccgaagccgg agcgcatcgc cgaatgcttg
    15661 ctcgcgaacg aactccaaac gggcatttcg ttttcgaacg cccgcgttca aacgttcgat
    15721 caacacttct gtcgctccca tgagctgctg agcctgatga cgcggcctgg gagggtcaag
    15781 gcgatttaga tggtgcgtcg gtccacggtg cccacggggc gagactgggc tccccctgcg
    15841 cttgacccag tagcaggtgg caggtagctc tccggccatg tccgaagttc gcgttcggtt
    15901 cgcaccatca cccaccgggt atctgcacat cggcggtgtg cgaaccgcac tatacagttg
    15961 gctctgggcg cgccaacaca agggcacgtt catcctgcgt atcgaagaca cggatcgcga
    16021 gcgcagcacc gaggagagca tccaggtcgt tttcgactcc atgcgctggc tcggcctcga
    16081 ctgggacgag ggtcccgaag tcggaggcga ccacggccct tatgtgcaga gcgagcggct
    16141 cgacatctac gcggactatg cgcagaggct gatctcctcg gggcatgcgt accgctgcta
    16201 tgccacgaaa gaagagatcg cggacgcacg cgaagcccac aagaagacgg gcaccaaaga
    16261 tggatttcgc tttcgaagcc cttggagaag ccaacctgaa cctgcggccg acccgaacgc
    16321 gccacacgtg attcgctttc gcgcgcccga ggaagggacg acgagctggg tggatgccgt
    16381 caaaggcaag atcgacatcc cgaacgcgac tctgcaagac tcgatcttgc tgaggcccga
    16441 tggaatgccg ctctacaact tcggctgcgt ggtggacgac ctcaccatgg gcgtcaccct
    16501 cgtcgcccgg ggcgacgatc acgtgatcaa cacggctccg cagattttgc tctatgaagc
    16561 tctcgaagcg ccggttccta cgttcgcgca catgcccatg gttctggcgc cgaatggaga
    16621 gaagctttcg aagcgccacg ctgctgttgg cgttctcgag taccgagaca tgggctatct
    16681 acccgacgca gtactgaact acctcgcgcg ccttggatgg tcgcatggcg accaggagat
    16741 cttcacgcga gcggagctga tcgagaactt cagctgggac cgcgtcgggt cgacggccgg
    16801 tcgctatgac gcgaagaaat tcctgcacgt ccaggccgaa caccttcgaa tgctgtccga
    16861 cgaagatctc gcgcgcggaa ctgtgccttt tctgcagaaa attggcatcg aggtcgccga
    16921 agacgacccg cttctcttga agacgattcc gtacgtgagg ccgcgcgctg aaacattcgt
    16981 cgacgtcgcg gaagcggtcg aatatttctt ccgtgacgta gagttcgaag agaaaggcaa
    17041 gcggaagttc ctcgtgccca gcagcgcgga gcatctcatg cgtctagcgg aagtcgtcga
    17101 agcggttgaa cccttcgccc aggggcccct cgacgaagcg gtcaaagcct ggctcgaagc
    17161 gaacgatctg cagatgaaag tcgtcgcgca gcctgcccgt gtggccatga ctggacgcac
    17221 gcgaagccct ggcctcttcg aagtgatgga ggtgcttggc aaggaacgaa ctttggcgcg
    17281 attgcgtttc ggcgcgcaga tcgcggcagc cccagaagcg cagtcctcgt agcgtcgctg
    17341 ctggtttccc gtattcttgg agctcactgt gcagacgctc gtcatcgttc tctacttggt
    17401 cactctggga gttttggctc tctacggttg tcaccgaagc gcattgctct tcttgtacct
    17461 gcgtcaccgc ggcgaacaga ctcttccgaa ggcgcgcttc gaagatgacc atttgccttt
    17521 cgtgacgatt cagctgccca tgtacaacga gctattcgtg gcggaacgtt tgatcgatgc
    17581 agtcgctcgg atcgagtacc cgcgcgagcg gctgcagatt caagtgctcg atgactcgac
    17641 tgatgaaacc tcgatcatcg ctgtggccaa gtgcaaagcg cttcgcaagc aagggatcga
    17701 cgtcgaatac ctgcacagaa ccgaccgaca cgggtacaag gccggtgcgc tcgcggcggg
    17761 gctcgagcgt gcgaagggcg agctcgtact cgtcttcgac gcggatttcg tgccgggaac
    17821 ggagatcctt ggtcagctcg tacacttctt caccgatcct cgagtcggaa tggttcaagc
    17881 tcgttgggcg catctcaacc ccgaccgatc tctgctcacg cgatgccaag cgatgctgct
    17941 cgacgggcac ttcgtcatcg agcatgcggc acgcaatcgt tcgggacgct tcttcaactt
    18001 caacggcacc gcgggaatct ggcgcaaaac ggcgatcaga gaagctggcg gctggcagca
    18061 cgatacggtc accgaggaca tggacctgag ctaccgcgct caactcgtgg gatggaaatt
    18121 cgtctacgca cctgaggtca gcgtgccagc tgagctcccc ggcgagatga acagcttcaa
    18181 aggccagcaa taccgctggg ccaagggctc cgttcaaacc gcccgaaagc tgctcggcac
    18241 gatcctgcga gcacccgttg gcgccaaagt gaagtcggaa gccttcttcc atctcagcaa
    18301 caacctggcg tacgtgttct tgctcgtgct ggccgcgctg caactgccca acatgttgat
    18361 ccgccgcaag atggagcacc ctgagctatt gatgctcgac gttccgctct ttctagcaac
    18421 ctgcggatcg gttgcggcat tctacgtcgt ggcacatcac gagctctacg gaggacgctg
    18481 gcaggcgatc aagcggcttc ccgcgatgat ggcgctcggt atcgggctct cgatcaacaa
    18541 cggacgcgcg gccatcgagg gattgtttgg ccgcgatgtc gagttcattc ggacgccgaa
    18601 gcgaggcacc atcgacggcg gaccctccgc agggacatcc cgctaccgtg gacgctggcc
    18661 ctggcacaac acgatcgagc tactgttcgg tctctattgt ttggcaacgc tcgccattgc
    18721 gatctcgact cggagttggg ccagcgtgcc gttcttgctg ctgttttgcg ccggattcac
    18781 gtatgtcggc gtcttgagct tgttcgaggc cttccagcgc aagcctggtc acggcgacga
    18841 ttcgacgcgc gccgagcaag agccgtcgac acactcttcc ccgaggccac agtcgatgtt
    18901 gagctgacac tccgctagca cttcactgct gcgcacgcag agattctgct cttcgaccgg
    18961 ggcacagaaa cgaatgctcg cgtcgatccg cgcgcactcc tcgtcagtct cgcaaggcgt
    19021 ccggcactgt ccgcggacac agagacgacc tggctgacat tcgttctcgg ggcagaactg
    19081 ctcggggcgc gtatcgggaa tgcactggaa cgagctgcag tactcgtgct cctcgcagtt
    19141 tgcatcctct gtgcaccgag tgagacaggt gtcatccgtg cacgggcggc atgcgcctgc
    19201 aacgcattgc tgggtatccg ggcaggggct gtctaggcag gtgatgagac actcgccgtc
    19261 gatgcagatg cgagaatcac cgcactcgaa gttgaattga caacggaaag cctggggcgc
    19321 tggatcttct tcgagaggat tgaggcatag gccgttctct tcgcaggtgg ttccgagggg
    19381 acagtcgtac tcgtcctcgc atgcacggac gcatccgatg gtcgtgcaga cttcccccac
    19441 cgcgcagtcg ggctctgcca cgcagatatt tcgttccggc gcaggcacgg aatcgcattc
    19501 gtcaccccgg cagaggaagc agccgcggtc actgagcggt ccgtcggcga tgcagacgta
    19561 gcgtgagtcc ctgtcgcagc cgacgaggcc gaagagtgca atgatgagaa tcccaaggcg
    19621 tttcatggga gcttcgacgc gccggaccag gggcgtattc actgagagga atcgcttgaa
    19681 accccagcaa ttccgttgaa taacaggggc gcaatgagtg gccacagcaa atggtcgaca
    19741 atcaagcggc gcaagggtgc acaggatgcc aagcgcggga aattgttcac caaagtcatc
    19801 aaggagatca ccatcgccgc ccggtcgggc ggtggggacc tcgagggcaa cgcccggctt
    19861 cgtcgcgcgg tggatgccgc tcgaaacgtg aacatgcccg cggacaacgt gacccgggcc
    19921 atcaagaagg gcaccgggga gctcgaaggg gttcaatacg aagagctact gtatgaaggc
    19981 gtcggcccag acggaacgct cttcatctgc gaagtcgtga cggacaaccg caaccgaacg
    20041 atcgccgaag tgcgcaagct gttcgacaag aaccacgggc agatcagtag cagcggcgcc
    20101 gcgctctggg cgttcgagca gaagggcatg atccgaatcg aaaaggacgc tgcgagcgag
    20161 gagaagctat tcgaggcggc ggtgggcggt ggcgcggaga acatggagct gctcgaggag
    20221 cagtgggtgg tgaccactgc gcgcaacgat ctcgattcgg ttcgcgatgc gatcgcctcg
    20281 gcaggcatcg aggtcagcga ggcaacgctg gagtacattc ctaacaatcc caaggtcgtc
    20341 gagggggatg aggcgatgaa gctcattcag ctctacgaag cgctcgaaga gcacgacgac
    20401 atccagaacg tgttttcaga tttcgagcta agcgagcaag cgcttgcaga gatggcctga
    20461 gagggtcatg cgcgtcctcg gcatcgatcc cggcagccgg cgcaccggct ggggtgtcgt
    20521 tcagctcgaa ggaacgcggc ttcttcacgt aggggccggc acaattgcgg tgccggagaa
    20581 gctcgccctc gagaagcgtc tgcagttgat ccatgcgggc ctcgagcagg tcatcgcgga
    20641 gcactcaccc gacgccgttg cggtcgaaga gatcttcttc gcgaagtatg caaatgcggc
    20701 cttgaagctc ggccatgctc ggggcatcgc cctgctggtc gctgcccaat ccgatttgga
    20761 agtccacgag tatccgccgg ccatcgtgaa gcgcacggtc gtgggccgag gggccgcaga
    20821 caaagttcag gtgacccgtc tcatcacggc tctgctcgga ctgaaggccg cccccgaaga
    20881 ggatgccgcc gatgccctgg cagtcgccat cacccacatc caagccagcc gctccaaagc
    20941 gatcgcccgc tgacgcggcg gcaccggcac ctgcggtggc acagtcgggc aacacagttg
    21001 ctggccctga cgctgttttt caacctctcc ctgtcggaaa tgaatacatc tgttcagtaa
    21061 gctgctacgc tccgccccgt gatcgggagg ctcagcggga cgatcgagca tcgggaggcc
    21121 gatggctcgg tgatcgtgga tgtgaacggt gtggggtacg aggtgtttct gccgcctggg
    21181 gccgcagggc gcctcggctc gaagccaggc gaggcggtta cgctggagat ccacacgcac
    21241 gttcgagaag acgcgatgac gctgttcggg ttcgccacgc ccgaagatcg cgctgcattc
    21301 cgagctcttc tcaaggtgag cagcatcgga ccgaagctcg cactcgcgat catcggcgtg
    21361 atgaacgcga gcgagttaca ggatgcgatc gcgcgccagg acaagaccgc attcaaagga
    21421 attaccgggg ttgggaagag gaccgtcgag cgaattctcg tcgatctgca agacaagctc
    21481 gacttcgccc cgaccggcag gactggggtg cgcttgcgcg cggttccgag caccggaagc
    21541 tcgatcgaag accgagtcgt tggtgccctg gtgcagatgg gatacaaacg aa
//
LOCUS       DASRZF010000265         5924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1576841, whole genome shotgun sequence.
ACCESSION   DASRZF010000265 DASRZF010000000
VERSION     DASRZF010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 5924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5924
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1576841"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VFG22_11705"
     CDS             <1..453
                     /locus_tag="VFG22_11705"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014804.4"
                     /note="catalyzes the ATP-dependent carboxylation of a
                     covalently attached biotin and the transfer of the
                     carboxyl group to pyruvate forming oxaloacetate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HET6416953.1"
                     /translation="VSEMRTGIDLVEAQIRVAGGERLWFSQEDIEIRGHAIECRINAE
                     DPSNGFAPSPGVIERWNVALAGDEHLRIDTHVESGYEVPPHYDSLICKVIAWGVDRDA
                     ACDRLSEALKSIRCDGISTTIPMHLAVLSSKDFRANRYDTRAVPGWES"
     gene            453..2183
                     /locus_tag="VFG22_11710"
     CDS             453..2183
                     /locus_tag="VFG22_11710"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HET6416954.1"
                     /translation="MAHVPVVPIGNTRDLVVDQATYEREHAAHGPSVSRLEARRAEVQ
                     AGWGAKYVDRVHEKDKLTTRERIERLRDPDSQVFEIGTFVNYGVDFDGLRSPAAGVVT
                     AFVRVEQRWCVVIANDNTVASGAWWPKTPEKIERAQEIARRLRLPALYLVDCSGLYLP
                     EQRKSFAGATGAGHIFKMNSLLSAEGVPQIAGVFGDCIAGGGYMPIISDRVYMTEQAY
                     MVIAGAALIKGAKSQHITSLDIGGPEVHVHQSGCADERVPDDVTLIDCMRREVSRLPS
                     SGAAFYRGGVESMEPAFSPAELPGLVPVDHRQAYEATEILARLCDQSLFWEVLPSVGR
                     EMICGIARVGGLYAGFVLNRQGLIDDPEANDEQRPAGILYRQGIAKISEFSRACDSDG
                     IPLIWLQDISGFDIGAEAERHGLLAYGSNLIYTNSTNETPMFTVLLRKASGAGYYAMA
                     GLPYDPVVQLSTPISRLSVMEGRTLAIASYNSKLDDNFEIVTKDPEERAKIETRMAEV
                     EARIEADMDPYVAARQMDTDEIVRLDELRDWLKVLVEMSYQGSGYRRIKNPRIWTMHD
                     LDVLVNGGRA"
     gene            2180..2770
                     /locus_tag="VFG22_11715"
     CDS             2180..2770
                     /locus_tag="VFG22_11715"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HET6416955.1"
                     /translation="MSGNAYVLGARSGEADTSELLAPTVGVFTAQVREGELVSAGQVL
                     GSIDVLGVLSELRAPEGVAGRVTSRIGGARTRVPVQYGDPLFTISAASMAPAAKAGVA
                     GNEGSAAALSFTAPMSGRFYSRPSPNDPPFVSAGDIVQRGQTIGLLEVMKTFNRLVYQ
                     GDALPERAVVEKIVPGEGDDVVRGDVVLALGPSPAS"
     gene            2787..3737
                     /locus_tag="VFG22_11720"
     CDS             2787..3737
                     /locus_tag="VFG22_11720"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HET6416956.1"
                     /translation="MPKASLILLLLLSSCRAPGRSSEAGDLTEVKGFVFKHEGIVRSV
                     GVFDPREPEELGLRPLVLVLHGGLGDDDDTVVLNFGKLNRLAAEDDFLVAYPQGLGGH
                     WNDGRAVDRYVAQRERVNDVGFLASLIDDLIVKREVDPKAVFIVGVSDGAMMAHRFAC
                     EQGAKLRGFTAVVGAMPYNVARRRARCGKAPISVLMINGTEDPIVPWAGGSVAFDGQE
                     LGKVLSAERTFEFWTRHDGCKDVAVTMIPDFVPSDGTRIERRQAIGCRDRSKVELFAV
                     QGAGHTWPSGWQYLPENMVGATSRDIDAAIAAWRFFQSTL"
     gene            3768..4955
                     /locus_tag="VFG22_11725"
     CDS             3768..4955
                     /locus_tag="VFG22_11725"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET6416957.1"
                     /translation="MLAPKLGDRSLFPDLEARAYLAHAAISPLSRPVCERVAEVARDY
                     ARGGMAGFVRWAPRLKEVRSGFARMIGATPNEIAFVANTTQGVIDIAFGLPWQKGERV
                     LLFNGEFPANVTPWQQAAEEFGLELCWLSLDAFHRSVEEGLAELEAALERGVRLVAVS
                     AVQYKTGLRMPIAEMAALCHRHGAEIFVDAIQALGATPVDVTSGIDYLSSGSHKHLMG
                     AEGAGFLYVAERCAGALRPRLAGWLGHEDPIAFLIGDSPQLRYDNPLQRGAPAFETGT
                     SNVIGLAAVGASIEILEQLGVPRIHAHVGGYLDLLESGLVELGFQSHRAKAPELRSTL
                     LSVTVPQDLRLSKLAAALRDKGVIANTPDGLMRFAPHWPNAHDEVPAVLGAVERALAE
                     LRA"
     gene            4971..5492
                     /locus_tag="VFG22_11730"
     CDS             4971..5492
                     /locus_tag="VFG22_11730"
                     /inference="COORDINATES: protein motif:HMM:NF016153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HET6416958.1"
                     /translation="MAAQSDRSFWIFNAMVSTAAVGFLGWLLLLREGGGIDADLGFMP
                     GVNATLNAASASLLLLGLAAIRSGRRKVHKRLMVSAFAASAVFLVGYVLYHYAHGDTP
                     YQGEGTIRTVYFTILITHVLLSIAMLPMILTTFYLAARARFAAHKKLARWTLPIWLYV
                     SVTGVVIYLMLRP"
     gene            5699..>5924
                     /locus_tag="VFG22_11735"
     CDS             5699..>5924
                     /locus_tag="VFG22_11735"
                     /inference="COORDINATES: protein motif:HMM:NF017451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HET6416959.1"
                     /translation="MGLIIQQRALEVAHGLRQVMPHVKKRDRSLFDQIHRAMNSVVLN
                     IAEADGNDLGTARARFASACGSAKEVRAGLQ"
BASE COUNT         1045 a         1807 c         1959 g         1113 t
ORIGIN      
        1 gtctcggaga tgcgaaccgg gatcgacctg gtcgaggctc aaattcgtgt ggccggtgga
       61 gagcggcttt ggttctctca ggaggacatc gagattcgcg ggcatgcgat cgagtgtcgc
      121 atcaacgccg aagatccatc aaacggcttt gccccgagtc cgggtgtcat cgaacgatgg
      181 aacgtcgcat tggccgggga cgaacacttg cgcatcgata cgcacgtcga atccggctac
      241 gaagttccgc cccactacga cagcctgatc tgtaaagtga tcgcttgggg cgtggatcgg
      301 gatgcggcct gcgatcgttt gtcagaggcc ttgaagagca tccgttgcga tggcatttcg
      361 acgacgatac ccatgcatct tgcggttctg agctcgaagg atttccgcgc gaaccggtac
      421 gacacgcgcg ccgtgccagg gtgggagagc tgatggcaca cgttcctgta gtaccgattg
      481 gaaacacacg ggatttggtc gtcgatcagg ccacctatga acgagagcac gccgcgcatg
      541 ggccctcggt ttcccgcctc gaagcgcgtc gcgccgaggt tcaagccggt tggggtgcga
      601 agtacgtcga ccgggtacac gaaaaggaca agctgacgac ccgcgagcga atcgagcgct
      661 tgagggaccc ggactcgcag gtcttcgaga tagggacctt cgtcaactac ggcgtcgact
      721 tcgacgggct ccgatcgccc gccgcgggag tggtgactgc cttcgttcga gtcgagcagc
      781 gctggtgcgt ggtgatcgcc aacgacaata ccgtggcatc gggtgcatgg tggccgaaga
      841 cgcccgagaa gatcgaacgt gctcaggaga tcgctaggcg cttgcgattg ccggcgctgt
      901 acctcgttga ttgcagtggt ctctacctgc ccgagcagcg caagagcttc gccggcgcga
      961 cgggtgctgg ccatatcttc aagatgaaca gcctgctctc cgccgagggg gtgccccaga
     1021 tcgctggtgt cttcggcgac tgcatcgccg ggggcggcta catgccgatc atcagcgatc
     1081 gcgtgtacat gaccgagcag gcgtacatgg tcattgctgg agcggccctc atcaagggcg
     1141 ccaagagcca gcacatcacc tccctcgaca tcggcggccc ggaggtgcac gtgcatcagt
     1201 ccggctgcgc ggatgagcgc gttccagacg atgtcacttt gatcgactgc atgcgccgcg
     1261 aggtatcgcg cttaccgagc tcgggcgcag cgttttaccg gggtggagtg gagtcgatgg
     1321 agccagcctt ctcgcccgcc gagcttcccg gtctggtccc cgtcgaccat cggcaggctt
     1381 acgaggctac cgagatcctc gcgcgtcttt gcgaccagag cctcttttgg gaggtcctgc
     1441 cctccgtcgg gcgcgagatg atctgcggca tcgcgcgcgt cggtggcttg tacgccgggt
     1501 tcgttctcaa ccgacagggg ctgatcgacg atcccgaggc gaacgacgag cagcggcccg
     1561 cagggatcct ctacaggcag ggcatcgcca aaatcagtga gttcagcaga gcctgtgaca
     1621 gcgacggcat tccactgatc tggcttcagg acatcagtgg cttcgacatc ggcgccgagg
     1681 cggaacggca cgggcttctc gcctatgggt cgaatctcat ctacacgaac agcaccaacg
     1741 agactccaat gttcaccgtg ctgctgcgga aggcatccgg tgccggctac tacgcgatgg
     1801 cggggcttcc ttatgatccg gtcgtgcagc tctcgactcc gatttcccgg ctgagcgtca
     1861 tggaaggccg aaccttggcg atcgcctcgt acaacagcaa gctcgacgac aacttcgaaa
     1921 tcgtcaccaa ggacccggag gagcgagcaa agatcgaaac ccgcatggcc gaggtcgagg
     1981 cgagaatcga agcggacatg gacccgtacg tggccgctcg gcaaatggac accgacgaga
     2041 tcgttcggct cgatgagctg cgagattggc tgaaagtcct ggtcgagatg agctaccagg
     2101 gcagcggcta tcggcgaatc aagaacccac gcatctggac catgcacgac ctcgatgtcc
     2161 tggtgaatgg gggacgtgca tgagcggcaa cgcctacgtc ttgggggcac gctccgggga
     2221 agccgacacg agtgagcttc tcgcgcccac ggttggcgtg ttcaccgccc aggttcgtga
     2281 aggggagctg gtttccgccg gacaggtgct ggggtcgatc gacgtgcttg gcgtgctcag
     2341 cgagctccga gctcccgaag gcgtggccgg ccgcgtcacg agccgaatag gcggcgcgcg
     2401 cacgcgggtt ccggttcagt atggcgatcc gctcttcacc atttcggcgg cttcgatggc
     2461 tccggccgcc aaggcgggtg tggccggcaa cgaaggttcc gcagcagcgc tttcgtttac
     2521 cgcaccgatg agcggccgct tctacagccg gccatctccg aacgacccgc cattcgtatc
     2581 ggcgggcgac atcgtccagc gcggtcagac gatcggcctg ctcgaggtga tgaagacctt
     2641 caaccggctc gtataccaag gtgacgcgct cccagagcga gcggtggtgg aaaagatcgt
     2701 cccgggcgaa ggcgacgacg tcgttcgcgg ggatgtggtc ctggcactcg gcccgtcacc
     2761 ggcgagctga agcgcggtga ggcgaaatgc ctaaggcctc attgattctc ttgctgctgc
     2821 tcagctcgtg tcgtgcgccg ggccgctcga gcgaggcagg cgatctcacg gaggtcaagg
     2881 gcttcgtgtt caagcacgag ggaatcgtgc ggtccgtggg cgtcttcgac ccgagggagc
     2941 cggaggagct gggtctccga ccactcgtct tggtcctgca cgggggtctc ggagacgacg
     3001 acgataccgt cgtgctcaat ttcgggaagc tcaaccggct tgcagccgag gatgattttc
     3061 tggtcgctta tccccaaggc cttggcggac actggaacga cggacgcgcc gtggaccgct
     3121 acgtcgcgca gcgggagcgc gtcaacgacg tggggttctt ggcatccttg atcgacgacc
     3181 tcatcgtaaa gcgcgaggtc gaccccaaag ccgtgttcat cgtcggggtc tcggatggcg
     3241 cgatgatggc gcatcgcttc gcctgtgagc aaggcgcgaa gctccgcggt ttcacagcgg
     3301 tcgtcggcgc gatgccgtac aacgtcgctc gccggcgggc gcgatgcggc aaggctccga
     3361 tttcggtctt gatgatcaac ggcaccgagg atccgatcgt gccctgggcg ggcggttcgg
     3421 ttgcattcga tgggcaggag ctgggcaagg tgctttccgc ggagcgcacg ttcgagttct
     3481 ggactcggca cgacggatgc aaggacgttg ccgtcacgat gatccccgat ttcgttccga
     3541 gcgatggcac tcgaatcgag agacggcagg cgatcgggtg ccgcgaccgc agcaaggtcg
     3601 agctcttcgc agtgcagggt gcagggcaca cttggccgtc gggatggcag tacctgccgg
     3661 agaacatggt gggcgcgacg agccgagaca tcgacgcagc aatcgctgcg tggcgtttct
     3721 ttcagagcac gctctagcgt cgcgcgcagg ggtgctagac tgcgcccatg ctggccccga
     3781 agctcggcga tcgcagcctc tttccggacc tcgaagcgag ggcctacctc gcgcacgcag
     3841 cgatttcgcc gctttcgcgt cctgtttgcg aacgggtcgc agaggtcgcg cgagactacg
     3901 caagaggagg catggcgggc ttcgtgcgat gggcgccgcg gctcaaagag gtgcggagcg
     3961 ggttcgcccg catgatcggg gcgactccga acgagatcgc gtttgtcgcc aataccaccc
     4021 agggcgtcat cgacatcgcg tttgggcttc cctggcaaaa gggcgaacgc gtcctgctgt
     4081 tcaatggtga gtttccggcg aatgtcacgc cctggcagca ggcggccgag gagtttggct
     4141 tggagctctg ctggctttcg ctcgacgcgt ttcatcgctc ggtcgaagaa ggcctcgcgg
     4201 agctcgaggc ggctctcgaa cgaggggttc gcctcgtcgc agtgagcgca gttcaataca
     4261 agacgggcct tcgcatgccg attgcggaga tggccgcgct ctgccaccgg catggcgccg
     4321 agatcttcgt cgatgcgatc caggcgctgg gggcgacccc cgtcgatgtc acctcaggca
     4381 tcgactacct cagctcaggc agccacaagc atctgatggg cgccgaaggg gccgggttct
     4441 tgtatgtggc cgagcgctgc gctggcgcgc ttagacccag gctcgccggc tggctcggcc
     4501 acgaagatcc gatcgcattc ctcatcggcg acagccctca gctccgatac gacaatcctc
     4561 tccaacgcgg cgcgccggct ttcgagaccg ggaccagcaa cgtgatcggt ctggcggcgg
     4621 tgggggcatc catcgagatc ctcgagcagc tcggcgtgcc gcgcatccac gcgcatgtcg
     4681 gcggctatct cgacttgctc gaatccgggc tcgtcgagct cggctttcag agccaccgcg
     4741 ccaaagcgcc ggagcttcgt tccacgctgc tcagtgtgac agttccgcaa gacctgcggc
     4801 tctcgaagct cgcggccgca ctgagggaca agggcgtgat tgccaacacg cccgacggtc
     4861 tcatgcgctt cgcccctcac tggccgaacg cgcacgatga ggttcccgcc gttctgggcg
     4921 ccgtggagcg ggcactggcc gagcttcggg cctgaaccta ggctggcacg atggcggctc
     4981 aaagcgaccg aagcttctgg atcttcaacg caatggtttc gacagccgcg gtcggattcc
     5041 tcggctggct tctgcttctt cgtgaaggcg gcggcatcga cgcggatctc ggtttcatgc
     5101 ccggcgtgaa cgcaacgctc aacgccgcct ccgcgtcttt gcttcttctc gggctagccg
     5161 cgatccgaag cgggcgccgc aaggtccaca agcggctgat ggtctcggcg ttcgccgcct
     5221 cggccgtgtt cctggtgggc tacgttctct accactacgc ccacggcgac acgccctacc
     5281 agggcgaggg aacgatccgc accgtttatt tcacgatcct gatcacccac gttctgctgt
     5341 cgatcgcgat gctcccgatg atcctgacaa cgttctatct agccgcgcgc gcacgcttcg
     5401 cagcgcataa gaagctcgcg aggtggacgc tgccgatctg gttgtatgtg tcagtgacgg
     5461 gggtggtcat ctacttgatg ctgcgtccat agcagtgtca gcggcagtgt cagtgccagt
     5521 gtcagcgcca gtgtcagcgg cagtgccagt gtcagcggca gtgccagcgg cagtgccagt
     5581 gtcagcgccg acgttcgtct gacttctctt tgtcatcgcg atctcctttt cgcctgggtg
     5641 tgggggcggg cttcgccctt tctgggaacc ccacacccag gcgaaaagga gatcactcat
     5701 gggactcatc attcagcagc gggcgctcga ggtagctcat ggacttcggc aggtgatgcc
     5761 gcatgtgaag aagcgggatc gttcgctctt cgatcagatt caccgagcga tgaatagtgt
     5821 cgtgctcaac atcgccgaag ctgacggaaa cgatctcggc acggcgagag cgcgcttcgc
     5881 gtcggcatgt ggctccgcca aggaagtgcg cgccggactc cagc
//
LOCUS       DASRZF010000266        20382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_198498, whole genome shotgun sequence.
ACCESSION   DASRZF010000266 DASRZF010000000
VERSION     DASRZF010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 20382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20382
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198498"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2245)
                     /locus_tag="VFG22_11740"
     CDS             complement(<1..2245)
                     /locus_tag="VFG22_11740"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HET6416960.1"
                     /translation="MSKSALLSLSELAASLWAALESANGLDRHTIETELVWCEVERGE
                     LGAAFSLMWEATDSSALAGQRQLTRAELQAHCGNDDAALAMVAEADEGDARVALVRAR
                     VAASRGDWESASRHASGATNAKLEGMRARLLAARADIELGQTARAMALLDNGDCKNSV
                     EELQVAALRAISDSDGREQALARIAERARDQGAMRVCAEAWADLSAVHSALGNSEEAR
                     SAAVRAVELWDDMATTLAPPLRAGFWSDTKRRDIRETSKRARARTGASRQTALESLLH
                     NLRRLASERDLDELLTVITDGAVALSGAERGFVLLVDESGALAAKTIRGAEDQLGPQT
                     AAFSRSIAETVLIDREPVVTVDAAQDARVQDYMSVHQLMLKSVACVPIQSRDRVWGVL
                     YLEHRSASGRFTGTDLSVLRAYGDQAAIAIETSHQVERIQAQKEALERANSALRDANA
                     HLEQRVLGQAAALQRTRKEIASLQAPTSDGDRWGLVGVSDGMRRVYEVLARVASNDVP
                     VVVTGESGTGKELVARAIHRGSARAEETYVSFNCGAIPEGLIETELFGHVAGAFTGAT
                     HAREGLFQQARGGTLFIDEIAEMPMRMQLDLLRVLQERRIRPVGGAVEIPVDVRLVTA
                     SKRPLEELVGDGTLREDLYYRLAVVELKLPPLRARRIDIPLLAEHFLERIAAERSQPR
                     KRLSRAALQRLGAHDFPGNVRELEHLLINAGVFASGGTIEAEDLAIESGRVTQSPSAG
                     ASNYVAFK"
     gene            complement(2266..2439)
                     /locus_tag="VFG22_11745"
     CDS             complement(2266..2439)
                     /locus_tag="VFG22_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416961.1"
                     /translation="MLPGFIIDQIRQREEEEQAKRQISQLELPLPMPEGSRPPKREDD
                     PDRGVVIIDLGAE"
     gene            2583..3425
                     /gene="rsmA"
                     /locus_tag="VFG22_11750"
     CDS             2583..3425
                     /gene="rsmA"
                     /locus_tag="VFG22_11750"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HET6416962.1"
                     /translation="MSGGRSILPDARDLLRKYDLHPKRSFSQNFLIQPAAVAQIADAA
                     AAFGTEVVELGPGLGALTQALLARGCRVLAVELDRDMVAVLHAELGEEPNLKIRQGDA
                     ADVDLTEHSRVCGSKLVVTGNLPYQATGAIIRRVVAEREVLSGAVLMVQREVRDRLVA
                     EPASKAYGALTVFTRAAFEVETVCRLRPGSFYPAPKVESAVVRLIPRATPLAEETESF
                     RMLVRAAFQQRRKTLRNALRALGDADRADQALETAGIDPARRGETLSIQEFSILAAAW
                     EASG"
     gene            3476..5305
                     /locus_tag="VFG22_11755"
     CDS             3476..5305
                     /locus_tag="VFG22_11755"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HET6416963.1"
                     /translation="MLRTALVWAALLILAGCAPKKIESPLEKAPPAEIAQARALASDG
                     DIRGAQNAYETFIISNQGTVEADLARVELGILGADMGRCQSAIPHFEQAQLSADRAIA
                     LRASLHLGACQLRLGDPDRGLETLQAVAGQRFSSEEQMLLWDTAVIAAEQTTSGALGL
                     SVMDTLLDRGGPAPDPQRADAAIDVLAQKLTIEDCATLVDDLRAGARPQVAVSMRLLD
                     HALETQDADLVLRSANILRESNAITEPEVSILVARADEFLHGNPYVVGALLPLSGRGR
                     EVGRQLLQGMQLAALDEGGPELVVEDTGGDPASTRAAAASLIGERGVIAILGPVESRT
                     TEAAAELTDPAGVPMLSFSASENVTSAGAHVFRFLYSPRDELAALVERARSQGLSRFV
                     ILYPDDGYGRTMERLFDQEVAAGGGIYCEGVAYPPGTKSFVGFVRTVLETSCDVVLFA
                     DVANQVALIAPTFAAEDAWSLRGSVIPEHVKRRIHFLLPSPSWSPELLARAERYLQGA
                     LVALPFYEGSETEINQHFRTAYETRYGRPPQMFDAYGYDAYRMISSTLRQGNQSREAL
                     AHALENGSGIRPVTSVDALSAERVPAHPPQVYEVRDGALQSVE"
     gene            complement(5340..6290)
                     /locus_tag="VFG22_11760"
     CDS             complement(5340..6290)
                     /locus_tag="VFG22_11760"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET6416964.1"
                     /translation="MTQARIREDGAWVGALLIATLATAPLAGCERGAPKQSNLQADTR
                     LVADVVARVGGRPIGAAEVEARMQSDGLSADDALGRLIDEELLVQEAERRGLVESPET
                     ERMIERLMVRAMLHDIENENTPESVTEQELRDDYALHADKFHIRERRRSWHILVKQAD
                     EAGRKRAQAILREIQRAENPRLVFERYAEAGSDDSGLEVKAEDLPELTRQANIESPYK
                     DALFSAKGEGPLNDVVKTSYGWHAIVVAEILPEETRTLADVEAESRERLSQRNRLAKL
                     VEIVQGLEAEGLVEYEEATVTRLLSTPHLPERASSAAAAR"
     gene            complement(6277..7962)
                     /locus_tag="VFG22_11765"
     CDS             complement(6277..7962)
                     /locus_tag="VFG22_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET6416965.1"
                     /translation="MRHRGWAVAVLIAALGLGCHAAEDDPEGQAGELSDPVRRENAIF
                     NLKKIYTETLAANGGDRNNAEVKAIADVTVGPLTSAYLAYPEDRINGLSILDLLYEMQ
                     DPRSLPALIEALNWRTEVSEDHAVRAAQTIQAMDVPAAERPKVIQALARSLERITDSR
                     PEDNKMRVSMIRALGSLKDKGATEILTNIATKQDEKQNFLINRLAAQQLGELRDPGAI
                     PSLIKCLFLFAPNQPELRMNDVAAEALILIGRPSLKPLLKVLAGDDASANAIAKQYID
                     AIKARRPDLANRMTVQQVTGGEASFALGALGFSEALGPLLGETTSSDTPRKLNAAIAL
                     VRIDVPDADRDRVRSALKNVYGDLPKGLAGVALRGQLLAAIAHTYDADMMPFIYDQVV
                     DAKTNPDVRLIAVQNYALLANKADAAKLSQAISNEQPSEAGGYREKFEERRPLLDLAN
                     ECDTNVECWISKAVSADGNKARKGAYMLGRYATGNAKAIEALVGQLGSEDLGVRLSAL
                     MALDQIAVDGSPAAVAKIDELRVREEGQSVWTRFRGEALPIQARLRSRAGDAS"
     gene            complement(8154..8702)
                     /gene="pyrE"
                     /locus_tag="VFG22_11770"
     CDS             complement(8154..8702)
                     /gene="pyrE"
                     /locus_tag="VFG22_11770"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HET6416966.1"
                     /translation="MDRLLQLLRERGFRRGQFVLSSGKTSDFFIDCKPAVLSAEGHAL
                     VGEALLERIRDALGPVSAVAGVELGGCPLASAVAGASWAHGEPIDAVYIRKSSKQHGT
                     RKMLEGADRLGPGDRLVIVEDTVTTGGSTLRAVDAVRDAGFEVAGVIAVVDRLEGGAS
                     AIRDAGLIFSTLYDRSDFMGDA"
     gene            8852..10171
                     /locus_tag="VFG22_11775"
     CDS             8852..10171
                     /locus_tag="VFG22_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HET6416967.1"
                     /translation="MDDLLAFFHDAEKPRDRWRVGTEAERIAVRRSSGAHLPYEGSVS
                     VVAIFEQLIGEHGWTAVRETENGPIIALHRDDASITLEPGSQIELSGAPYRSVHEGKA
                     ESDAHWASLQPVMDDLGLVWLGLGCHPFASVEELGWVPKLRYAVMREYMPTRGSMAGD
                     MMTKTCTVQANLDYESEEDAMRKLRVGLRAQPIVTAMFANSPWEGGKRSGYRSYRALI
                     WLHMDPDRSGLLPFAWKDQPRYLEYVNWALDAPMFLVKRDGKAYYNTGQTFRKFMEEG
                     FRGLRANYGDWIAHLSTLFPEMRLKNTLECRGADVQRPELLFALPALWKGLLYDDSSL
                     SALEGLVDSWSFPEVQRQREALARHAVRTKFMNRDAIDWAGEMLELAEAGLRRIGDRN
                     QRGEDETVLLEPLRRLLEASSCPADVLLQQLGDEVPSREAVIRTAAL"
     gene            complement(10168..11811)
                     /locus_tag="VFG22_11780"
     CDS             complement(10168..11811)
                     /locus_tag="VFG22_11780"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET6416968.1"
                     /translation="MRIGTSYKQHPGLADQWDAIVIGSGIGGLTAAATLSKLAGKRVL
                     VLERHYTAGGFTHTFRRPGYEWDVGVHYIGDVSHPRAMARRMFDFITDGQLEWADMGE
                     VYDRIIIGDNSYDFVKGYENFRAKLHGYFPTEKDAIDRYLAKVTTTVKKAQLYFTEKA
                     VPPLVSSLFGGMMRRPVLKEAMRTTREVLEELTQNQELIAVLTGQFGDYGLVPSKSSF
                     FIHSMVVAHYFRGAAYPVGGSGRIAETILPVIERAGGEVVTNAEVTEILIENERAVGV
                     KLADGRELRAPTVISNAGVVNTFERLVPTEPARAAGWGSKAVELGHSIAHLSLYVGLK
                     GTTEELQLKKTNLWVYRDNRHEENFAKFEADINAPLPLVYLSFPSAKDPDFERRYPGH
                     STIEAITLGRYENFKAWENTSWKKRGAEYDELKASLSERLLQTLYKHAPATRGNVEIA
                     ELSTPLSTRNFAAHPHGEIYGLQHTPARFEQRWLRPRTPIKGLYLSGADTVSAGVVGA
                     LMGGMICSSAVLKGNVLGQVLKHQAQTLQTKTPVPAPLG"
     gene            complement(11832..12425)
                     /locus_tag="VFG22_11785"
     CDS             complement(11832..12425)
                     /locus_tag="VFG22_11785"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6416969.1"
                     /translation="MPRAALTTEALNAFRGRAVAAATKLFAERGYRGVTLRSLARELE
                     VSPMTPYRYFANKEELFAMVRTEAFRRFADVQRDAIANVDDPDEVLRTLGKAYVGFAL
                     DEPDAYHIMFELLQAPAGTYPELEREQARAFSYLHAAVNAAVDAGLIEGEALTRAHYL
                     WAQVHGLVSLHLAGKLVMGRSFDELVSAVFEKPEAAQ"
     gene            complement(12502..13725)
                     /gene="tyrS"
                     /locus_tag="VFG22_11790"
     CDS             complement(12502..13725)
                     /gene="tyrS"
                     /locus_tag="VFG22_11790"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HET6416970.1"
                     /translation="MNPEAQLAALKRGVVDLHVESELLERLNTGKPLKIKAGFDPTRP
                     DLHLGHTVLLGKMRQFQELGHEVIFIVGDFTAQIGDPSGRSSTRPVPTRDEILEGART
                     YAEQAFKILDRDRTRIVYNGEWLGKMAFDDVIRLAGKYTLARMMERDDFKRRWSEHQS
                     ISLHELLYPLAQAYDSVVLECDVELGGTDQLFNLLVGREIMREMGQRPQIVMTTPILE
                     GTNARSENGKIVGSKMSKSLDNYIGVAEPPKEQFGKLMSISDELMWRYYELLSSQATL
                     EIQRRKRACAAGEMNPRDAKIDLGREIVTRYHDAEQASAAESAWLAQFSQRQVPQDMP
                     ERELAIDEDAVWVPKLLSDLGLVKSSSEGRRRIQQGGVEVDGDRLTDPQAKLPKGRRY
                     VIKAGKRSWVAVTLK"
     gene            complement(13735..14061)
                     /locus_tag="VFG22_11795"
     CDS             complement(13735..14061)
                     /locus_tag="VFG22_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET6416971.1"
                     /translation="MGKESMTKSELIDAVAQRTKITKSRAEQVVNCVFNAMTSALEQG
                     EGIEIRGFGSFTVREYKSYSGRNPRTGKPVPVPEKRLPFFKVGKELKELVNGGVAEAG
                     AAEPGE"
     gene            complement(14215..15402)
                     /locus_tag="VFG22_11800"
     CDS             complement(14215..15402)
                     /locus_tag="VFG22_11800"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET6416972.1"
                     /translation="MRDEDDISQKTLAVHGGEPRTYPYDALVAPIAQTATYSFADTAE
                     LIAYFEGRKEREEYGRYGNPSVRLVEEKLAALERTEDAVAFASGMAALTSTILALVKS
                     GSHVILFADCYRRTRQFVTTFLDRFGVSSSLIPSADVDALEAAIRPETRLVIAEAPTN
                     PYQTVPDLERLVKICKAAKVKTLIDSTFATPVNFRPASYGIDIVIHSATKYLAGHNDV
                     LGGVMAGNAGIVSLVRDLRSVFGACLDPHAAYLIHRGIKTLALRVEQQNASAQALAER
                     IEQHPRVKQVWYPGLPSHPNHETATRLMSGFGGVVTFELDADLQGTSRFVDACRIPRI
                     APSLGGVESLIEQPALMSYFELSTQEREAIGIRNNLVRYAVGIEDTSELIADVLAALD
                     RTG"
     gene            complement(15488..19630)
                     /locus_tag="VFG22_11805"
     CDS             complement(15488..19630)
                     /locus_tag="VFG22_11805"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HET6416973.1"
                     /translation="MTGPMRTVLAACVAVSMSAAGCGGGTTANGHVAPKISSTPPSTA
                     TVGVPFNYTVTAEGMTPVTFAVESGPDGFSIHPTSGVVTWTPQTEGTVSVEISATNLA
                     GSDSQAFDVDVEGLTGPVFTTEPPTEATVAAQYAYDPDVVANGAVSWSVPVAPNGLAI
                     DPDTGAVRWTPSSDQAGMNAVTVRATEDESGLFADQQFTVTVVDTGGPAFITSTPPSR
                     VYAGEVLSYSAAASGAPTIRWTIEAPSSGNPASGVQIVTNPPEGTAVTVQWDTASVGA
                     GDYSIALQVDNGLGSPNVQEFVITVDPRPPVPEIDLVTTPPPTTIFVGNQYDYDVNLT
                     PGTESAGVTWSLTGPTEPAGLPITIDPNTGEVSFTASVANGEIEYGYTVRAENVLGEG
                     DEATITVDAIFPPATPMLSVTPSNTFTLAVGEIFPGASASASGNPSPVLSISGALPSF
                     LEFDPLTGLLSASVSQPAPEEADIGTYAFDIVATNSEGVDSQTIDIEVIAAPPTVESI
                     TPAAGRRQSDVPIVVRGTGFVSAVTPVIRLQLGAYSENLVTTYVDEGTLTATVPLDLG
                     RPSGVYDVVVDQGSTSTLSKRFTVTEGDGTILGGTVGVDITLTAVDSPHVITSDVRIE
                     NGATLTVQPGAVLMIAGATNRRIDVGVNGPGALVANGGEPGVGDQIVITRFQDVGGPA
                     PTGHYRGLRFGANIVSASTLLQNVVIEFGGRVDAGANHGAVEVLSGSAPQIADTIIRE
                     SLNFGLYAQDGAGSDASDWFHDNQLTANGRSPINIFSNDVSTLGANLRLSGNGQDRVY
                     VRGLAVNRPSASWANYGVPFYLHNGLSVRGGSIMSIAPGTEMRFRSGQQFQVSTNAEA
                     GTLVATGTPQSPIRMTADSGTWLGLRLDDRVQGGTVLRNVRMEAFDSGVSGGLRIDNP
                     ANPGDRVAIAERCLFQSAEPGAVGVYLSDNAGFGSFESNVMDVDGISVSAPMSGFQDV
                     IGPSNTYEASLRVRAGVLDGKNAVWSKPTASDGSARSIRPTGNLTVANGSLTIRAGNQ
                     IEMPLNGGLTISDAQLLVDGSSSEPVVFMPAPGVAYWNRIRLRGAGGGGASRISHAML
                     QSAGSNPTLAQSTDRAAIVVQANAGIPATPAVENTIIADSNGYGMTFSDSTHCQDMCD
                     DNTIVGSRFSALRMYANFIGRFGVGNLLSGNNTSGTLGHEGVWVVGDTVDTSATWPAN
                     DAPYVIQGDIELRSPSPLNPVPVWTLDPGAELRFASDRRIRVGEGNDGILDARGTSTA
                     PITFTSIDTATPSFWRGIEFSQGSDGSILDWVLVSYGGRSANTGNVNFRMGSAVTIGA
                     ATFTHSEYYAAAIYAGSAPMFTGPPTDRVYMFNGQASNPGAGDPLFDCVRDIAADTCS
                     EP"
     gene            complement(19727..19915)
                     /locus_tag="VFG22_11810"
     CDS             complement(19727..19915)
                     /locus_tag="VFG22_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416974.1"
                     /translation="MEMLNQIDPKLQREVRRRQAEFWDSCLWALAEALEHAFPRVRSV
                     EPRISAEEVAADLAYGRD"
     gene            20035..>20382
                     /locus_tag="VFG22_11815"
     CDS             20035..>20382
                     /locus_tag="VFG22_11815"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET6416975.1"
                     /translation="MFNWDDVRYFLTLQRHGTLAAAGAALTLDPTTVGRRLMKLEEEL
                     GARLFDRTPNGYVLTDAGHRLLPRAERIERETLGVERDVAGEDQKLEGVVRLTATEML
                     TTRFIAPHLRRFRE"
BASE COUNT         3607 a         6477 c         6280 g         4018 t
ORIGIN      
        1 ctttgaaagc gacgtagttg ctcgctcccg cgctcggcga ttgcgtcact cttccactct
       61 cgatcgcgag atcctccgcc tcgatcgtac cgcccgaagc gaacacgccg gcgtttatca
      121 agagatgttc gagctcccgg acgtttccgg ggaaatcgtg cgcgcccagt cgctgcaacg
      181 ccgcgcggct gagccgcttc ctcggttggg agcgttcggc ggcgatgcgc tctaggaagt
      241 gttccgcgag caaggggata tcgattcgcc gcgcgcgcag agggggtagc ttcagctcca
      301 cgacggcgag ccggtagtag aggtcttcgc gaagcgtgcc gtcaccgacg agctcctcga
      361 gaggccgttt ggacgcggtg accagacgta cgtcgactgg gatctccacg gcgccgccca
      421 caggacgaat tcgcctttct tggagcacgc gaagcaagtc gagctgcata cgcatgggca
      481 tttccgcgat ctcgtcgata aacagcgttc cgccgcgggc ttgctgaaag agaccctcac
      541 gcgcgtgggt cgcgccggtg aacgcgccgg cgacgtgccc gaaaagctcg gtttcgatga
      601 ggccctccgg aatcgcgccg caattgaatg agacgtaggt ctcctcagcg cgcgcgctgc
      661 cccgatgaat cgctcgagct acgagctctt tccccgttcc gctttcaccg gtgaccacca
      721 ccggcacgtc gttcgacgcc actcgggcga gcacctcgta aacccgccgc atgccatcgc
      781 tcacccccac cagaccccat cgatcaccgt ccgaggttgg agcctgcagc gaggcgattt
      841 ccttacgagt tcgctggagc gccgcggcct ggcctaggac tctctgctcc aaatgcgcgt
      901 ttgcgtcgcg cagcgcagag ttggcccttt cgagcgcctc tttctgagct tggatgcgtt
      961 cgacttggtg ggaggtttcg atggcgatgg ctgcctggtc gccataggcc cgcaagaccg
     1021 acagatcggt acccgtgaat cgcccgctcg cacttcggtg ctcgaggtac agcacccccc
     1081 agacccggtc tcgggattgg atcggtacgc aggcaaccga cttgagcatg agctggtgga
     1141 cggacatgta atcctgaacg cgagcgtcct gcgccgcgtc caccgtcacg accggctcgc
     1201 ggtcgatcag cacagtttcc gcgattgagc gactgaacgc ggcagtctgg gggccgagct
     1261 ggtcttccgc gccacgaatc gtcttcgccg ccagcgcccc agattcgtcg accagcagca
     1321 cgaacccgcg ctcggcgcca ctgagcgcga cagcaccgtc ggtgatgacc gtcaaaagct
     1381 cgtccaagtc gcgctcgctc gcgagtcgtc gcaggttgtg gagcagcgac tccaaagcgg
     1441 tttgccgcga cgcacctgtc cgggccctcg cacgcttgga ggtttcgcga atgtctcgcc
     1501 gcttcgtgtc agaccagaag cctgcgcgca ggggcggcgc tagcgtggtg gccatgtcgt
     1561 cccaaagctc gacggctcgg acggcagcac tgcgggcttc ttctgaattt ccgagcgcgc
     1621 tgtgaactgc gctgaggtcg gcccaggcct cggcacagac acgcatcgct ccctgatctc
     1681 gtgcccgctc tgcgatcctc gcgagggctt gctcccgacc atcgctgtcg gagatggcac
     1741 gtagcgcggc cacctggagc tcctcgaccg aatttttgca atcgccgttg tcgaggagcg
     1801 ccattgcccg tgctgtctga ccgagctcga tatcggcgcg agcggcgagg agccgggcgc
     1861 gcatgccttc caacttcgca ttcgtcgcgc ccgacgcatg cctcgaggcc gattcccagt
     1921 cgcctcgtga tgcagcgacc cgggcgcgca ccagcgcgac acgcgcgtct ccctcgtccg
     1981 cctccgcgac catcgcgagc gcagcatcat cgttgccaca atgagcctgt agctcggcgc
     2041 gggtgagctg ccgttggccc gcaagggctg acgaatctgt ggcttcccac atgaggctga
     2101 acgcagctcc aagctcgccg cgctccacct cgcaccaaac gagctcggtc tcgatcgtgt
     2161 gccgatccaa gccatttgcg ctctctaaag ctgcccacaa ggacgcggcg agctcgctca
     2221 ggctcaataa ggcactcttt gacacacccg cagtctagcg gatcgtcact cggcgcccag
     2281 gtcgataatc acaacaccac ggtcgggatc atcctcccgc tttggtggcc tcgagccttc
     2341 aggcatcggc agaggtagct caagctgaga aatctggcgc ttcgcctgct cttcttcctc
     2401 acgctgacgg atttggtcga tgatgaagcc tggaagcatt tgttcctctg cccttaacgt
     2461 aggtcaatcc gcgggttcga ccaatataat aacgccttct ctagtgacaa cggctgtgac
     2521 acaagcgaga ctccattgcc ccattcaagc caaaaattgc atgattatag agagttgacc
     2581 gcatgagcgg cggccgcagc atcctacccg atgcaagaga tctgctaagg aaatacgacc
     2641 tgcacccgaa gcggtcgttt tcccagaatt tcctgatcca gcctgctgct gtagcgcaga
     2701 tcgcggatgc tgcggccgcg tttggaactg aggtcgtcga gcttgggccg ggtctgggtg
     2761 cgctgaccca ggccctactc gcgcgcggct gcagagttct ggctgtggag ctcgaccggg
     2821 acatggtggc cgtcctgcac gcggagcttg gcgaggaacc gaatctaaag atccgccaag
     2881 gcgatgccgc agacgttgat ttgaccgagc attcgagggt ttgtgggtca aagctcgtcg
     2941 tcacgggaaa ccttccctat caagcgacgg gtgcgatcat ccgcagggtc gtcgcggagc
     3001 gcgaggtgct cagcggggcg gttctgatgg tgcagcgtga ggtccgggac aggctggtcg
     3061 ccgagccagc tagcaaggcg tacggagcac tgaccgtctt cacgcgtgcc gccttcgagg
     3121 tcgaaacggt ctgtcggctg cggcccggct cgttctatcc tgcgccaaaa gtcgagagcg
     3181 ccgtggtccg gctcattccg cgcgccacgc ctctggccga agaaacggag tcctttcgaa
     3241 tgctagttcg ggcggcgttc cagcagcgtc gaaaaacact gcgcaacgcg ctgcgggcgc
     3301 tgggcgacgc cgatcgggcc gaccaggctc tcgaaacagc tggaatcgac ccagctaggc
     3361 gaggtgagac gctttcgatt caagagtttt cgatcctcgc tgcagcctgg gaggcttccg
     3421 gctgagctgg ccgctacgtg gcagcggggc gaccatccat gtatcgtgcg cccgcatgtt
     3481 gagaactgcg ttggtatggg cggcgctgct gatcctcgcc ggctgtgccc ccaagaagat
     3541 cgagagccca ctcgagaagg cgccgcctgc cgaaatcgct caagcgcgcg ctctggcttc
     3601 ggacggcgac atccgaggag cgcagaatgc ttatgagacg ttcatcatca gcaaccaagg
     3661 gaccgtcgaa gcggaccttg cgcgcgtcga gctcgggatc ctcggcgccg acatgggacg
     3721 ctgtcagagc gcgattccgc acttcgagca ggcgcagctg agcgcagacc gggccattgc
     3781 cctccgcgca tcacttcatc tgggagcttg ccaactgcgt cttggggatc cggaccgggg
     3841 cctcgaaaca ttgcaagccg tggccggaca gcggttctcc agcgaagagc agatgcttct
     3901 ctgggatacc gcggtgatcg cggcggaaca gacgacgagc ggggcgctcg gtctcagcgt
     3961 gatggacact ctgctcgatc gcggtggccc ggcccccgac cctcagcgcg ctgatgccgc
     4021 gatcgacgtg cttgctcaga aactgacgat cgaagactgc gccacgctcg tcgacgatct
     4081 ccgcgcgggt gcgcgacctc aggtggcggt ttcgatgcgc ctcctcgatc atgccctcga
     4141 gactcaggac gcagacctcg tgcttcgcag cgcgaacatc ttgcgcgaga gcaacgcgat
     4201 cacggagccc gaggtgtcga tcttggtcgc tcgcgcagac gagttcctac acgggaaccc
     4261 gtacgtcgtg ggggccctgc tgccactgag cggccgggga agagaagtgg gccggcaact
     4321 gctccagggc atgcagcttg cggcactgga tgaaggaggc ccggagctcg tcgtcgaaga
     4381 caccggcggc gatcccgctt ccacccgagc cgccgcagca tcgttgatcg gtgagcgggg
     4441 cgtgatcgcc atactcggtc ctgtcgagag ccgcacgacg gaggcggccg cggagctcac
     4501 cgacccagcc ggtgttccca tgctgagctt ctctgcctcg gaaaacgtga cgagcgcagg
     4561 tgcccatgtc tttcgttttc tctacagtcc tcgagatgag ctggccgctc tcgttgaaag
     4621 ggcgcgctcg cagggtctct cgcgtttcgt gattctctat cccgacgatg gctacgggcg
     4681 aaccatggag cgtttgttcg accaggaagt ggcggccggt ggcggcatct actgtgaagg
     4741 cgtcgcttac cctcctggga cgaagtcatt cgtcggcttc gtgcgcaccg tcctggagac
     4801 gagctgcgat gtcgtccttt tcgccgatgt ggccaaccaa gtcgctctaa tcgcgcccac
     4861 tttcgctgcc gaagacgctt ggagtctcag aggaagcgtc attcccgagc acgtcaaacg
     4921 cagaattcat ttccttcttc ccagtccgag ctggtcccct gagcttctcg ctcgcgccga
     4981 gcgatacctc cagggcgcgc tcgtggcgct gcccttctac gagggctcgg aaaccgagat
     5041 caaccagcac ttcagaaccg cttacgaaac ccgctacggg cggccgccgc aaatgttcga
     5101 cgcatacggc tacgacgcct accgaatgat ttcatccaca ctccggcaag ggaaccaatc
     5161 gcgggaggcc ctcgcacacg cactcgagaa cggctctggg attcgtccag tgacttccgt
     5221 cgatgcgctt tctgccgaac gcgtcccagc gcatccacct caggtgtacg aggttcgcga
     5281 cggggcgctt cagtcggtcg agtgacgggc gggagacctg gactcgacgc ccgcgacgcc
     5341 tagcgcgcgg cagcggcgct ggatgcccgt tcgggtaggt gcggcgtaga gagcaagcgt
     5401 gttacggtgg cttcctcgta ctcgacgagg ccctccgctt cgagtccttg gacaatctcg
     5461 acgagcttgg ccaaccgatt ccgctgactc aaacgctcac gcgactcagc ttcgacatcg
     5521 gcgagcgtcc gagtttcttc ggggaggatc tccgccacca cgatggcgtg ccagccatac
     5581 gaagtcttga cgacatcgtt caatgggccc tcgccttttg cactgaagag agcgtccttg
     5641 tacgggcttt cgatgtttgc ttgtcgcgtg agctccggga gatcttcagc cttgacctcc
     5701 aatccggagt catcactccc agcctccgcg tagcgctcga acacgagtcg aggattctcc
     5761 gcgcgctgga tctcgcgaag aatcgcttgc gcacgcttgc gccctgcttc gtctgcttgc
     5821 ttgacgagga tgtgccacga gcgtcgccgt tctcgaatgt ggaacttgtc ggcatgcaag
     5881 gcgtaatcgt cccgaagctc ctgctccgtg accgactccg gcgtgttctc gttctcgata
     5941 tcgtggagca tcgcgcggac catcagacgc tcgatcatcc gctccgtctc agggctttcg
     6001 acgagtccgc ggcgctccgc ctcctgcacg aggagctctt catcgatcag ccgaccgagc
     6061 gcatcgtccg cgctcagccc gtcggattgc atgcgcgcct cgacctcggc agcgccgatc
     6121 ggccggccac ccacgcgggc caccacgtcg gcgaccagcc tcgtgtctgc ctgcagattg
     6181 ctctgtttcg gggccccgcg ctcacacccg gcgagcgggg cagttgctag cgtggcaatg
     6241 agcagggctc ccacccaggc tccgtcctcg cggattctag cttgcgtcac cggcccgact
     6301 gcgcagacgc gcttggatgg gcagcgcttc accccggaac cgagtccaga ccgactgacc
     6361 ctcttcccgg actcgaagtt catcgatctt cgccaccgca gcggggcttc catcgaccgc
     6421 gatttggtcg agcgccatca gtgccgacag gcggactccg aggtcctcgc tgccgagttg
     6481 accgactaag gcctcgatgg ccttcgcatt ccctgtggcg tagcgtccaa gcatgtatgc
     6541 gccctttcga gccttgttgc cgtcggcgct cacggccttc gagatccagc actcgacgtt
     6601 ggtgtcgcat tcgttagcca gatcgagcaa gggtctccgc tcttcgaatt tctctcggta
     6661 gccgccagcc tccgagggct gttcgttcga gatcgcttgc gagagctttg cggcgtccgc
     6721 tttgttcgcg agaagcgcgt agttttgaac ggcgatcagg cggacgtccg ggttggtctt
     6781 tgcgtcgacg acttggtcgt agatgaaggg catcatgtcg gcgtcgtacg tgtgcgcaat
     6841 ggccgcgagt agctgccccc gcagcgcgac gcccgcaagc ccctttggca agtcgccgta
     6901 gacattcttg agcgcagagc gaacgcgatc gcgatcggca tcgggtacgt cgatcctcac
     6961 gagggcgatc gccgcgttca gcttgcgggg ggtgtccgaa gaagtggttt ccccaaggag
     7021 cggacccagg gcttcggaga atccgagcgc accgagcgca aagctcgcct ctccgccggt
     7081 gacctgctgc acggtcatgc ggttcgcgag gtccggccga cgagccttga tagcgtcgat
     7141 gtactgcttg gcgatcgcat tggcactcgc atcgtcgccc gcgaggacct tgagcaatgg
     7201 cttgagcgag ggtcggccaa tcagaatcag cgcctcggca gccacgtcgt tcatgcgcag
     7261 ctccggttgg ttcggcgcaa agaggaacaa gcacttgatc agcgagggaa tcgcccccgg
     7321 atctcgcagc tcacccagct gctgcgccgc cagacggttg atcaagaagt tttgcttttc
     7381 atcctgcttc gtagcgatgt tcgtgaggat ctccgttgcg cccttgtcct tgagcgagcc
     7441 cagcgctcga atcatcgata cgcgcatctt gttgtcttcc ggacgcgagt ccgtgatgcg
     7501 ttcgagagag cgcgccaaag cttgaatcac cttcggccgc tctgccgccg gcacgtccat
     7561 cgcttggatc gtctgagcgg cccgaactgc gtggtcctcc gaaacctcgg ttcgccagtt
     7621 cagtgcttcg atcaatgcgg gcaagcttcg cgggtcttgc atctcgtaga ggagatccaa
     7681 gatcgagaga ccgttgatgc ggtcttctgg atacgcgagg tacgcgctgg tgagcggacc
     7741 gaccgtgacg tctgcgatcg ccttcacctc cgcattgttt cggtcgcccc cgttggcagc
     7801 gagcgtttcg gtgtagatct tcttgagatt gaagatcgcg ttctccctgc gaaccgggtc
     7861 ggagagctca ccggcttgtc cttccggatc atcttccgcg gcgtgacaac ccaacccgag
     7921 cgctgcaata agaactgcaa ccgcccaccc cctgtggcgc ataatcattc tgaacctccc
     7981 cacgcggccc gtttccccat ttgttacgaa ggcttacgcg actacagcat gataacgagg
     8041 caggatcgtg cctgtcaaac cttcttcaag ctgagcagaa gtggcgcagc cacgaatttc
     8101 agattccgca ggaactgccc agcaatcagg cgcattttcg ccccttggac ggctcacgcg
     8161 tcgcccatga agtcgcttcg gtcgtacaga gtgctgaaga tgaggccagc gtcgcggatc
     8221 gccgacgcgc cgccttcgag acggtcgacc accgcgatga ccccggccac ttcgaaaccc
     8281 gcgtcccgca cggcgtctac tgcacgcagg gtcgagccgc ccgtggtcac ggtgtcctcc
     8341 acaatcacca gcctgtcacc gggtcctagg cgatctgcgc cttcgagcat ctttcgagtg
     8401 ccgtgctgct tgctcgactt ccggatgtag acggcgtcga tcggttcacc gtgcgcccag
     8461 gaagcgccgg cgaccgccga agcgagggga cagccgccta gctcgacgcc tgctaccgct
     8521 gaaacggggc ctagcgcgtc gcgaattcgt tccagcaagg cttctcccac gagagcatgg
     8581 ccctcggcgg aaagcacggc gggtttgcag tcgatgaaga agtcgctcgt ctttccagag
     8641 ctgagcacga attggccgcg gcgaaaaccg cgttcccgga gtagctgaag caatcgatcc
     8701 acgggtctgt tccaccacgt ccgagccgcc cgcgcaagcg tccgcccggc tcgtcgcggg
     8761 ggcatcgctc gtgccttcgt ggtagggtcc cgcgccccca gggtcgaact cagaggacac
     8821 gttgccgaaa ctcgatgctc cgctacgcgg gatggatgac ctgctcgcgt tcttccacga
     8881 cgcggaaaag ccgcgtgacc ggtggcgggt cggcaccgaa gccgaacgaa tcgcggttcg
     8941 acggagctcc ggcgcgcacc tcccctacga gggctccgtc agcgtcgtgg cgattttcga
     9001 gcagctcatc ggcgagcatg gctggacggc cgtgcgcgag accgagaacg gcccgattat
     9061 tgctctgcat cgcgacgacg cctcgatcac tctcgaaccg ggatcacaga tagagctttc
     9121 aggtgcccct taccgttccg ttcacgaggg caaggccgag tccgatgcac actgggcaag
     9181 cctccagcca gtcatggatg accttggcct cgtttggctc ggcctcggtt gccacccgtt
     9241 cgcgtccgtc gaagagctcg gctgggtccc caagctgcga tacgccgtga tgcgggaata
     9301 catgccaacc cggggctcga tggccgggga catgatgacc aaaacctgca ccgtgcaggc
     9361 caacctcgac tacgagtccg aagaggacgc gatgcgcaag ttgcgcgttg ggcttcgcgc
     9421 gcaacccatc gtgaccgcca tgttcgcaaa cagcccctgg gagggaggaa agcgaagcgg
     9481 ctatcgctcg tatcgcgctc tcatttggct gcacatggat ccagatcgat ccgggcttct
     9541 gcccttcgcc tggaaagatc agccgaggta cctggagtac gtgaattggg cgctcgatgc
     9601 gccgatgttt ctagtgaagc gcgacggcaa ggcctactac aatacggggc agacgtttcg
     9661 gaagttcatg gaggaaggct ttcggggact ccgggcaaac tatggcgact ggatcgcgca
     9721 cctgagcacc ctctttcccg aaatgaggct caagaatact ctcgagtgcc gcggtgcgga
     9781 cgtgcagcgc ccggaattgc tcttcgccct tcccgcgctc tggaagggtc tcctctacga
     9841 cgactcgagc ttgagcgccc tcgagggcct ggtggattcc tggtctttcc ccgaagtgca
     9901 gcggcagcgt gaggcgctcg ctcggcatgc cgtgcgcacc aagttcatga atcgcgacgc
     9961 gatcgattgg gccggcgaga tgctcgagct cgccgaggcg ggattgcgca gaatcggcga
    10021 ccgcaaccag cggggagaag acgaaaccgt tctactcgag cctttgcgtc gtctcctcga
    10081 agcatctagc tgccctgcgg atgtattgct ccaacagcta ggcgacgagg tcccgtcccg
    10141 cgaggcggtg atccgcacgg ccgcgctcta gccgagcggc gccgggaccg gcgtcttggt
    10201 ctgcagcgtc tgcgcttgat gcttcagaac ctgtccgaga acgttccctt tgagcaccgc
    10261 agagctgcaa atcatcccgc ccatcaacgc gccgacgaca cctgccgaga ccgtgtctgc
    10321 tccactgagg tacaagccct tgatcggagt gcgcggacga agccagcgct gttcgaagcg
    10381 cgccggcgta tgctgaaggc cgtagatctc tccgtgcggg tgagcggcaa aattgcgtgt
    10441 tgagagaggc gtcgagagct ctgcgatctc cacatttcca cgggttgccg gcgcatgctt
    10501 gtaaagcgtc tggagcaagc gctccgacaa gctggccttg agctcgtcgt actcggcgcc
    10561 gcgcttcttc caagatgtgt tctcccaggc cttgaagttc tcgtagcgtc cgagggtgat
    10621 cgcctcgatg gtcgaatggc cagggtaccg tcgctcgaaa tccggatcct tcgcggaagg
    10681 aaaagagagg tacacgagtg ggagcggcgc gttgatgtcg gcctcgaact tggcgaagtt
    10741 ctcctcgtgc cggttgtccc ggtacaccca tagattggtc ttcttcagtt gaagctcctc
    10801 ggtggtaccc ttcaagccca catagaggga cagatgcgcg atcgaatgac ccaattcgac
    10861 cgccttgcta ccccatccgg ccgctcgtgc tggctccgtg ggcaccaatc gctcgaaagt
    10921 attcacgacg cctgcattgc tgatcaccgt cggtgcgcgc agctcgcggc cgtcggcgag
    10981 cttcacgccc accgctcgtt cgttctcgat cagaatctcg gtgacctcgg cgttggtgac
    11041 gacctcgccg ccggcgcgtt cgatcactgg aagaatcgtt tcggcgattc ttcctgaacc
    11101 gcccactgga taggctgcgc cccggaagta gtgagcgacg accatcgaat ggatgaagaa
    11161 gctgctcttc gaaggaacca gtccatagtc accgaactgc cccgtgagca cggcgatgag
    11221 ctcttggttc tgtgtgagct cttcgagcac ctcgcgtgtg gttcgcatgg cctctttgag
    11281 aacgggcctg cgcatcatgc cgccaaacaa gctcgacacg agaggcggca ctgccttttc
    11341 ggtgaagtag agctgtgcct tcttcaccgt cgtcgtgacc ttggcgaggt agcggtcgat
    11401 ggcgtctttt tcagtcggaa agtacccgtg gagcttcgct cggaaattct cgtacccctt
    11461 cacgaaatca taagaattgt ccccgatgat gatgcgatcg tagacctccc ccatgtcggc
    11521 ccattcgagc tgcccgtcgg tgatgaagtc gaacatgcgc cgggccattg cgcgcgggtg
    11581 cgagacgtct ccgatgtaat ggactccgac gtcccactcg tatcccggcc ttcgaaacgt
    11641 gtgcgtgaag ccgcctgccg tgtagtgccg ctcgagcacg agcacgcgct tgcctgcgag
    11701 cttggagaga gtggcagccg ccgtgagccc tccgatgccg gacccgatga cgatcgcatc
    11761 ccactgatct gcgagaccgg gatgttgctt gtacgatgtt ccaatgcgca tgaacctctc
    11821 ctattgacgc gtcactgcgc cgcttccggc ttctcgaata ccgcgcttac caactcgtcg
    11881 aacgaacgac ccatcacgag ctttcccgcg aggtgcaatg agaccaggcc atggacttga
    11941 gcccagaggt aatgcgcacg cgtgagagcc tccccttcga tgaggcccgc gtcgacggcc
    12001 gcgttcaccg ccgcgtgcag ataagaaaaa gcgcgcgctt gctctcgttc gagctcgggg
    12061 tacgtccctg caggggcctg caggagctcg aacatgatgt gatatgcatc gggctcgtcg
    12121 agggcgaagc ccacatacgc cttgccgagg gttctcagca cctcgtccgg gtcgtcgacg
    12181 ttggcgatcg catcgcgctg cacgtcggcg aaccggcgga aggcctcggt tcgaaccatt
    12241 gcgaagagct cttccttgtt cgcgaaatac cggtacggcg tcatggggct gacctcgagc
    12301 tcccgcgcca aggacctcag ggtcacgccc cgatagccgc gctctgcgaa gagcttcgtc
    12361 gcggcggcga cggcccgccc ccgaaacgcg ttcaacgctt ccgtcgtcaa agccgcgcga
    12421 ggcatcagga gccatttacg tcgtaaaatg atccgggtca accgagaacc gagcgctgcc
    12481 tttttgggga gcaggctcgg cctatttcaa agtgacggcg acccagctcc gtttgccggc
    12541 cttgatgacg tagcggcgtc ctttagggag cttggcctgg gggtcggtga ggcgatctcc
    12601 gtcgacttcg acgccacctt gctggatacg gcgccgccct tcgctcgagc tcttgaccag
    12661 gccaaggtcg ctgaggagct tcggaaccca gaccgcatct tcgtcgatgg caagctcgcg
    12721 ctccggcatg tcttggggca cctgccgctg tgagaactgc gcgagccagg cggattcggc
    12781 ggcgctggcc tgctcggcgt cgtgataccg ggtgacgatt tccctcccga ggtcgatctt
    12841 cgcgtcccga gggttcatct cgcctgccgc acaagcgcgc ttgcgccgtt ggatttctag
    12901 cgtcgcctgc gaggagagca actcgtagta gcgccacatc agctcgtctg agatgctcat
    12961 cagcttgccg aattgttcct tgggcggctc ggccacgccg atgtaattgt cgagggactt
    13021 gctcattttg gagccgacga tcttcccgtt ctccgaacgc gcattcgtgc cttcgagaat
    13081 gggcgtggtc atcacgattt gaggacgctg ccccatttcg cgcatgatct cccgcccgac
    13141 gagcaggttg aaaagctggt cggtgccccc tagctcgacg tcgcattcca agaccaccga
    13201 atcgtacgcc tgcgccaggg gatagagtag ctcgtgaagc gaaatacttt ggtgctccga
    13261 ccagcgccgc ttgaaatcgt cgcgctccat cattcgcgca agcgtgtact tgcccgccaa
    13321 acgaatcacg tcgtcgaacg ccatcttgcc gagccactcg ccgttgtaga cgattcgggt
    13381 gcggtctcga tcgaggatct tgaacgcttg ctccgcgtag gttcgcgctc cttcgaggat
    13441 ttcatctcga gtcggcacgg gtcgagtgct gcttcgcccc gaggggtcgc cgatctgcgc
    13501 ggtgaaatcg ccaacgatga atatgacttc gtgtcccagc tcttggaact ggcgcatctt
    13561 gccgaggagc accgtatgtc cgaggtggag gtccggccgt gtgggatcaa accccgcttt
    13621 gatcttgagg ggcttcccgg tgttgagacg ctccagtagc tccgactcga cgtgcaagtc
    13681 gaccacaccg cgcttgagcg cggcgagctg cgcttcgggg ttcatccgta gcctctattc
    13741 gccaggctcg gcggcaccgg cctcggcgac gcccccgttg acgagctcct tgagctcttt
    13801 tccgaccttg aagaatggaa ggcgcttctc gggaacaggc actggtttgc ccgttcgagg
    13861 gtttcgcccg ctataggact tgtactcgcg gacagtgaag ctaccaaacc cgcggatctc
    13921 gattccctcc ccctgttcga gtgcagaggt catcgcgttg aacacgcagt ttacaacctg
    13981 ttcagcacga cttttggtga tcttcgtccg ctgggctaca gcgtcgatga gctccgactt
    14041 cgtcatcgat tctttcccca ttgggcgctg agtatatcca ggcacccagg ccggtcaata
    14101 gcaatttcgc gcacttgcaa ccttcttcgc tacccgggtt caaagtgccc ctcagtggca
    14161 aaggagagtt ccagcggggt tttgtgaggc gaatcgcaga gacgaagggg ggcctcagcc
    14221 cgtccggtcc aaagcggcca gcacgtcggc tatgagctct gaggtgtctt cgatgcctac
    14281 ggcatatcgc acgaggttgt tgcggatccc aatcgcctcg cgctcttgag tgctgagctc
    14341 gaaatagctc atcaaggctg gctgctcgat caaggactcc accccgccga ggctaggggc
    14401 gattcgcgga atccggcagg catccacgaa ccggctggtc ccctgcaggt cggcgtcgag
    14461 ctcaaacgtc accaccccac caaaaccgct catgagccgc gtggcggttt cgtgattcgg
    14521 atgcgagggt agcccaggat accagacctg tttcactcgg ggatgctgct caatccgttc
    14581 tgcgagtgcc tgagctgagg cgttttgctg ctcgacacga agggccaggg tcttgatgcc
    14641 gcggtgaatc aggtatgccg catgcgggtc gagacacgcg ccgaacacac tcctgaggtc
    14701 ccgaaccagg gaaacgatgc cggcattgcc agccatcact ccgccgagga cgtcgttgtg
    14761 tccggccaag tacttcgttg cgctgtggat gacgatgtcg atcccatagc tcgccggcct
    14821 gaagttcacg ggggtcgcga aagtgctgtc gatcagggtc ttgaccttcg ccgccttgca
    14881 gatcttcacc agccgctcga gatcaggaac ggtctggtac ggattggtgg gcgcctctgc
    14941 aatgaccaat cgggtctcgg gcctgatcgc ggcttcgagc gcatcgacgt cggccgatgg
    15001 gatgagagag ctcgaaacgc cgaacctgtc taggaacgtc gttacgaact gtcgcgttcg
    15061 gcgatagcag tcggcgaaga gaatgacatg cgaccctgac ttgacgaggg ccaggatcgt
    15121 cgatgtcaac gccgccatac cgcttgcaaa agcaacggcg tcctctgttc gttcgagcgc
    15181 cgccaacttc tcttccacga ggcgcaccga gggattgccg taccgcccgt actcttcgcg
    15241 ctctttgcgc ccctcgaagt aggcaatgag ctccgccgtg tcggcaaagg aataagtcgc
    15301 ggtctgcgcg attggagcca ccagggcgtc gtacggatac gttcgcggtt caccaccgtg
    15361 aaccgccaga gtcttctgtg aaatgtcgtc ctcgtcccgc acgaagacgg gagcctagga
    15421 gagcggatct ggcggcgcaa gcgcagggac cgaaaccaaa atcaggaccg tccccttttc
    15481 gaggactcta tggctcgctg caggtgtccg cggcgatgtc gcgcacgcag tcgaagagag
    15541 ggtcgccggc tccggggttc gatgcctgcc cattgaacat gtagacgcgg tcggtcggcg
    15601 gaccggtgaa catcggagca gagcccgcat agatcgccgc cgcgtagtac tccgagtgcg
    15661 tgaacgtcgc cgccccgatg gtaaccgcag agcccattcg gaagttgacg ttgcccgtgt
    15721 tcgcgctgcg tccaccatag gagacgagca cccagtcgag gatcgagccg tcggaccctt
    15781 ggctgaattc aatccctcgc cagaacgacg gcgtcgccgt gtcgatgctg gtgaaggtga
    15841 tgggcgctgt gctggtgccg cgcgcgtcca ggattccatc gttgccctcg cccactcgaa
    15901 tgcgccggtc gctcgcgaaa cggagctctg ccccgggatc gagggtccac acaggaaccg
    15961 gattcaaggg actcggactt cggagctcga tgtcgccctg tatcacgtag ggcgcgtcgt
    16021 tcgccggcca ggtcgcgctc gtgtcgacgg tgtccccgac tacccacacc ccttcatgac
    16081 ccaacgtgcc cgatgtgttg ttgccgctca gtagattgcc cacgccgaac cgtccgatga
    16141 agttcgcgta catccgcaac gccgagaagc gtgatccgac gatcgtattg tcgtcgcaca
    16201 tgtcttggca atgagtggaa tcggagaacg tcatgccata gccgttgctg tctgcgatga
    16261 tcgtattttc gacggccggg gtcgccggaa tgcccgcgtt tgcttgcacg acaatcgcgg
    16321 cccggtccgt tgactgcgcc agtgttggat tcgatcctgc agattggagc atcgcgtggc
    16381 tgatgcgtga cgcgcctcca ccacccgcac cgcgcagacg gattcgattc cagtacgcca
    16441 caccgggcgc tggcatgaac actaccggct cgctcgacga accatcgacg agcaactgcg
    16501 cgtcgctgat cgtcaggcca ccgttgagag gcatctcgat ttggttaccc gcgcgaatgg
    16561 tcaaggatcc gttcgcgacc gtgaggttgc ccgtgggtcg aatcgatcgg gcagacccgt
    16621 cactggcggt aggcttgctc caaaccgcgt tctttccgtc gagcacgccc gcacgaacgc
    16681 ggagcgacgc ttcataggta ttcgacggac cgatgacgtc ttggaaccca gacatcggag
    16741 cgctcaccga gatgccatca acatccatga cgttgctctc gaatgagccg aagccggcat
    16801 tatccgacag gtagacgccc accgccccgg gctcggcgct ctggaagagg catctctcgg
    16861 cgatggcgac tcgatcgccc gggttggcgg ggttgtcgat tcgtaagcca ccgctcacgc
    16921 cagagtcgaa tgcctccatg cgtacgtttc tcaggaccgt gccgccctga acgcgatcgt
    16981 cgagtcgcaa gccaagccag gtcccactgt ccgcggtcat tcgaatcggg gactgaggcg
    17041 tgcccgtagc cacgagtgtc cccgcctcgg cgttcgtcga aacttgaaac tgctgaccgg
    17101 agcgaaaccg catttcggtt ccgggcgcga tcgacatgat gctaccgcca cgaacgctga
    17161 gaccattgtg caagtagaaa ggcacgccgt agtttgccca gcttgcgcta ggtcggttga
    17221 ccgccaagcc ccgcacgtag acgcggtctt ggccgttgcc gctgagtcga aggttggcgc
    17281 cgagcgtcga cacatcgttc gagaagatgt tgattggcga gcggccattc gcagtgagct
    17341 gattgtcgtg gaaccaatcg ctcgcgtcgg aacccgcgcc atcctgtgcg taaagtccga
    17401 agttgaggct ctctcgaatg atcgtgtctg cgatctgagg tgccgatccg ctgagaacct
    17461 ccactgcgcc atggttggca ccggcatcga ctctgccgcc gaactcgatg actacgttct
    17521 gaagcagcgt cgatgccgag acgatgttgg cgccgaagcg gagcccgcgg tagtggccgg
    17581 tgggcgcggg gcctccgacg tcttggaagc gggtgatcac gatctgatcg ccaacaccgg
    17641 gctcgccccc gttggcgacg agcgcgcccg gaccattgac accgacgtcg atgcgccggt
    17701 tcgtcgctcc cgcgatcatc aagaccgcgc ctggctgcac ggttaaagtc gcaccgttct
    17761 cgattcgaac gtcgctcgtg atcacatgcg ggctgtcgac cgcagtgagt gtgatgtcga
    17821 cacccacggt gccaccgaga atcgtgccat cgccttcggt caccgtgaac cgcttggaca
    17881 gcgtgctcgt cgagccctga tcgacgacta cgtcatagac acccgacggg cgccccaaat
    17941 ccagcggcac cgtggcggtg agcgtgccct cgtctacgta ggtcgtgacg aggttctccg
    18001 aatacgcgcc gagctggagg cgaatcaccg gtgtcaccgc gctcacgaag ccagtgccac
    18061 ggaccactat tggaacgtcg gactgtcggc ggcctgcggc aggcgtgatc gattcgacgg
    18121 tgggtggcgc agcgatcacc tcgatgtcga tcgtctggct gtccacgcct tcgctgttgg
    18181 ttgccacgat gtcaaaggca taggtgccga tgtcggcctc ttcgggagct ggctggctga
    18241 ccgaagcgga gagcaagccg gtgagcggat cgaactcgag gaagcttgga agcgcgcccg
    18301 agatgctgag cacggggctg ggattgccgc ttgcgctggc cgaggccccg gggaagattt
    18361 cgccgaccgc cagcgtgaat gtgttgcttg gcgtgacgct gagcatcggg gtcgctggcg
    18421 ggaagatcgc gtcgactgtg atggtagcct cgtctccctc gcccaggacg ttctccgctc
    18481 gaaccgtgta gccgtactcg atctcgccgt tcgccacaga ggccgtgaag ctcacctctc
    18541 cggtgttggg atcgatcgtg atcggcagac cggcgggctc ggtcggtcca gtcaggctcc
    18601 atgtgacgcc tgcgctctcg gtacccggag tgagattgac gtcgtagtcg tactggtttc
    18661 caacgaagat ggtagttggc ggcggagtcg tgaccaggtc gatctctggg accggcggtc
    18721 gcgggtccac cgtgatgacg aactcctgaa cgttgggact gccaagcccg ttgtcgacct
    18781 gcagagcgat gctgtagtcc ccggccccta cggacgcggt gtcccactgg acagtcaccg
    18841 cggtgccctc gggcgggttc gtcacgatct gaacgcccga cgccgggttc cctgacgacg
    18901 gtgcctcgat ggtccaccgg atcgtaggtg cgccggaggc agcggccgaa tagctcagca
    18961 cctcgccagc gtagacgcga ctcggcggcg tcgaggtgat gaacgcgggt ccgccggtat
    19021 cgacgacggt caccgtgaat tgctgatcgg cgaacaggcc gctttcgtct tccgttgcac
    19081 gaaccgtcac agcgttcatg cccgcttgat cggagctcgg tgtccatcga actgcaccgg
    19141 tatccggatc gatcgccaag ccgttgggcg ctacgggcac gctccaggac acagcgccgt
    19201 tggcgacgac gtccgggtca tatgcgtact gcgccgctac ggtcgcctcg gtcggaggct
    19261 cggtcgtgaa gaccggtccg gtcaaccctt ctacgtcgac gtcgaacgct tgcgaatcac
    19321 tccccgccaa attggtggcg ctgatctcga ccgagaccgt gccttcggtc tgcggcgtcc
    19381 aggtcacgac tccgctcgtt ggatggatgg agaacccgtc cggtccgctc tccactgcga
    19441 acgtgactgg cgtcattcct tcggcggtaa ccgtgtagtt gaagggaacg ccgacggttg
    19501 ccgtgcttgg cggagtggag cttatcttgg gcgccacatg tccgttggcg gtggtcccgc
    19561 cgccacagcc tgccgcgctc atcgaaacgg cgacgcacgc cgccagcact gttcgcatcg
    19621 gtccagtcat cggtcccctc cgcttgccga attactatgc taacagatat ttacgttgaa
    19681 aatgctgttt cctcctgaat ttatggcgtt ttctatcctt gcctgttcaa tcgcgaccgt
    19741 aggccaaatc ggcggcgacc tcttcggcac taattcttgg ctccaccgat cgtacccttg
    19801 ggaaggcgtg ctccaaggcc tcggccagcg cccataggca ggaatcccag aactccgcct
    19861 gccgtctgcg aacctcgcgc tggagcttgg ggtctatttg attgagcatc tccatgttca
    19921 actcctttca gctttcatat gaaggactta gcgcctcatc agcgccgttt gacgcagttc
    19981 aaaagtgcgt ttttgcagtt gcgaagcgct ctgtgcgctg cgatgctgca ggctatgttt
    20041 aactgggatg atgttcggta cttcctgaca ttgcagagac acgggaccct ggccgcagcg
    20101 ggagctgcat taacgctgga ccccaccacg gtcgggcggc gcttgatgaa gctcgaggaa
    20161 gagctcggag ctcgcctctt cgacagaacc cccaatggct acgtactgac cgacgccggg
    20221 catcgactgc ttccgcgtgc cgagcggatc gaacgagaga cgctcggcgt ggagcgggat
    20281 gtcgcgggag aagatcagaa actcgagggc gtggttcggc tcaccgcgac ggagatgctc
    20341 accacgcggt tcattgcgcc gcaccttcga cgttttcgcg ag
//
LOCUS       DASRZF010000267        19701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3298018, whole genome shotgun sequence.
ACCESSION   DASRZF010000267 DASRZF010000000
VERSION     DASRZF010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 19701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19701
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3298018"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..4644)
                     /locus_tag="VFG22_11820"
     CDS             complement(133..4644)
                     /locus_tag="VFG22_11820"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET6416976.1"
                     /translation="MAGSYSMPPPGEETHARRQITVIVLLLFGMVALYQFEQFAQHTF
                     DPSGMLAFGFVVLASYTIGGLVGQIRLPHITGYLIAGLVFGPSLAKALTGFDLPAPFD
                     KGILNEEVIEQLSLFDTLAVALIALTAGGELKLEGLKKGFRAISSILAAQIVSVGVLV
                     TAFFWLISGAVPYVGLPGVAGLPMAAALAVGAMVASVSLATSPAATIAVITETRAIGM
                     MTKNVLSVVVLKDVIVVVAFAVAQVVVAQQIGIGALEGGIASYLLQHIVISILFGAVV
                     VGGLMALYIRYVNQELLIFVVGVVYLVAFISKQLDWDLVLVFLAAGFGVSNFSKTGHR
                     LIETVERLSLPVYVVFFTLAGAKLHLDELRQVALFALGLVTVRGFAMFSGTKVGARFG
                     KADEATQAFGWMGFVSQAGVSILLAAIVGNRFGELGRSLETLIIGGVAINELIGPVLF
                     KLGLSLAGETSSHRKSMAPSRHLSVKPPSMLPDDAGAAKQLEPWPEHVGEKNLWGLPL
                     ETSSAELNSRTQELAGDLQSIVREVSTGPLREFQMEAGKYLRDLRREFLRHHRRLTVQ
                     ARAEEGAERDELVTMLRSAQSDLAAHWRGIVLGRSVTLAKLTWTPESILDNLDALVDE
                     LPEVVAAPFEELSYESKKDDSLWKSVRRQGLRLRRSWRKTFGQAAPTRDVHLRALGRY
                     HLSYDAPVRLEGLAALFVEADRHLAARTRSLFDGVIAGYDDIVEISSDSGADLEGQLV
                     RLRHEVEEELALALDETVRITGDGTHRAASALATGFVRIKEDLPTFGTLDLPASERRT
                     SRRFANRMRAMETLTKDVSNLRKASAAEYSSLAMELELLGLEARIKDHVAEYVVRLTN
                     TVKRRATLQVDRVSETLHKALEELDTELESNYSGDELAVILRESTEPTSKITGEAARV
                     TTELYQDLSDDSKVAPLLDTLVDACRGLTPRYRVTVGQRQTGEWRLPTALPEVEVPFR
                     EVVLTYIDSRVAPKLLASRRELAERVQPLSGLLQELERVVAFNVEIATAELEIVHDEE
                     IPEEMHTLLRDMVYGQLERSYNSLEEIRAEAQGWPAMIEREMRDASLGTLQTLRGELA
                     EGEFSKSRLDAMRRAASGLRMIEKAERLPRMKRLKSQFRGGLRAIFGEERLGRWSERL
                     GLTATLPSDALSAGTFAAAEVVADLPLVYRRLFAADTMEAGDVLTGRDEEIRRAESVL
                     SSEIRGRLRSVALVGVDGVGKAAVSSAIVRSRRWKNVKRVVFTAPVSLEDVDAIFQDA
                     PEGQLVVVDGLHWMLSMAPGGFDPLRRFVKGIIEEGGRRRWLTHGAVLFWNFASTVAP
                     LRDAFPEIVRLEPLDQEALQAAVIARHRLSGFEHSFDRGDGSPIEGLFARSASRIRRP
                     YDQYFHELHAATGGLVRDALRLWLASIRGIQDDIVHVGPIPASSYARVRRLPDDILVN
                     LYQIARQGWMDAAGQARLFRLDVNTAQAQLSRMAHLGLLEEREGSFVIAVHLRGVLGR
                     VLAERRWVL"
     gene            complement(4644..6134)
                     /locus_tag="VFG22_11825"
     CDS             complement(4644..6134)
                     /locus_tag="VFG22_11825"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HET6416977.1"
                     /translation="MSTQSRDFRGVIRPVGAVVLGIGVMIALCAGVGMVWDQFAPDPT
                     RPQGGAASLALSAIIVSIVGLAAFLYGNRFVSERISRREALLAVSLIWIAAGACGAVP
                     FMLGAGMSPADSFFESVSGLTTTGATVISDIEGRLSRPLLLWRSLIQWLGGMGIVVLF
                     VAVFPNLGAGGKHLFRGEVPGTTAEGLRPRIAETSFTLWKLYAAFTILEIALLKILGL
                     DLFDAVCHAFTTMSTGGFSTLDSSVGGFNSAAIEYVIACFMLIGSVNYGLYYGLLRTG
                     SWKTLFRNIEFRWYLLIVGFSVIVLTLLNLSVHDELLTSFRYSFFMVATTISSTGYGT
                     DDYSAYSSTALAVVVLIMFVGGCSGSTAGGIKVERAVLMGKQAIAQVQKSFRPSTVEL
                     VRMGKAAISTQVLADVASFFLIYMGCIAFGLLFVTLVEGVPIPTAFGAMLTCLSNMGP
                     APFHVGPDNFASYSAGSKMFFSLAMLLGRLEFFTLFALILPGFWRR"
     gene            complement(6131..7471)
                     /gene="trkA"
                     /locus_tag="VFG22_11830"
     CDS             complement(6131..7471)
                     /gene="trkA"
                     /locus_tag="VFG22_11830"
                     /inference="COORDINATES: protein motif:HMM:NF007031.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HET6416978.1"
                     /translation="MNIVIIGLGEVGRHLLSVLDKEGHDIIAVDSSASAVQYAEEHYD
                     VASIVGYGASETLLKIAGVGRADLVAAVTNHDEMNLIAALASKQLGAKHVVARVQGTA
                     WSEWTEGIRYGLLGVDVVINPRVLVAQELSRIARSYGASDVVDLAQDRVELVQIDLGE
                     SRVLNKPLSSVELPAHTLVSAVVRDGELFVPGGADVLVKGDRVYLIGRPEGVLAAERL
                     FSKERKARRVCIVGGGVVGKALARELLQQQATVMMIESDLASAEEISAEFQGVEVVHG
                     DGTDRALLEEQEVGTYDLFAAVTRQDEINLMAALLAQRAGCKRTATIVQRADYTSIYR
                     QLGIDIVLSPRTVASDQILRFSRGGGLHNLTVLEDGQAEVVELTVSPKCRAVNKPLHR
                     MNLPRGSLLAAIVHGDDVIIPHGKDIVQAGDRAILMTTKEARASVERLFRPRGE"
     gene            complement(7468..8808)
                     /locus_tag="VFG22_11835"
     CDS             complement(7468..8808)
                     /locus_tag="VFG22_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6416979.1"
                     /translation="MAGRLILAALSLLWATSPGVVRAASESGAEGGAGELGTIVMLVL
                     VIGVAYVLAHNVVERLQHRFLVVSGAEYLLLGFLLGPAFPAIHALDNIAGLLPIIALA
                     AGWVGLLRGTDFDFASLQRLDPATWRVVILHHLIPGVAVGVGAYYFFTESGWVAATPR
                     DAALSAAALACFAAADSAEPFDLLARRYEMSGRLVGLLRNGTRLGDILVILAFGLIFC
                     VFHENSPEAQDYSPALWAWVTVLLGAALGFLFSLFLAGDESDNSRFLALVGIIAFASG
                     GAYFLELSPLAVNLSMGFVLVNFARGGPLLHSTLESTERPMAIVLLIFAGALWQPTPL
                     VPTLLGLGGFLIARTGAKWLASVIAGWGTSLRKDLFRGLLAHGDVTLAMAVSFRLVYE
                     GDAAKIAYTVVLASVVLNDLIAPRLLRGLLVDEGELQRELKEPESQTTVEVAAR"
     gene            complement(8867..9532)
                     /locus_tag="VFG22_11840"
     CDS             complement(8867..9532)
                     /locus_tag="VFG22_11840"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6416980.1"
                     /translation="MDEERRPRRAGPVERPGAHGGKRDINRRKRVQDLVDAGLTLFLA
                     RGIEAVTIDEVAREAGMAKGNFYRYFRDKADLVEAVIEPVASVMLDAIQECETKLREV
                     NSRDEAVAVYSQLGNRLAGALAMHPDAMCLYLQERRAPATTARQAISDLANDLDNRAM
                     TLSQTAVDRKFVSISDPRVSALAVLGAVEALALASVRNQIQLDPVETTKGLISIVLEG
                     LRG"
     gene            9754..10710
                     /gene="lipA"
                     /locus_tag="VFG22_11845"
     CDS             9754..10710
                     /gene="lipA"
                     /locus_tag="VFG22_11845"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HET6416981.1"
                     /translation="MSLPARKPDWLRVRLPAGPEQDEFQSLKAKFRSRGLHTVCEEAR
                     CPNIFECWGQGTATVMILGETCTRGCRFCSVKTGKPGGIVDPQEPENTALSLAEMGLA
                     YVVLTMVDRDDLDDGGAAHVSETVRRIKQHSPDLRVETLVGDFLGKRDDVRTLVQEGR
                     PDVFAHNVEVVPRLQRTIRDARCSWDRSIDTLAAAKELGSAVTKSSLMVGLGEQGDEV
                     LEAMRFLRAADVDVLTIGQYLRPTKRHAPVQRYVDPDEFLDYERAGLEMGFRYVASGP
                     LVRSSYRAAEAFLHGLASEKATAGERPPMNRYGRKRKLSVLG"
     gene            10718..11800
                     /locus_tag="VFG22_11850"
     CDS             10718..11800
                     /locus_tag="VFG22_11850"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET6416982.1"
                     /translation="MQVIRDDGSLDPQLDPGLSIDHVVALYKAMVRTRVIDDRMEKIQ
                     RQGRIAFHVGSLGEEAAILGAVSAMRDQDWIVPCYREVGALLWRGYPLQKYVDHMYGN
                     AGDPVHGRQMPDHSFSRAHRYLSVSAPIGTQIPHAVGLAMAAKKRGLDESAAVFFGDG
                     ATSSNDFHAAMNFAAVYQAPVLFLCRNNGYAISLPVAQQTATRTLAEKAAAYGVPAAR
                     VDGNDALAVWGAVRRGAERAAEDGGPTFIEMMTYRRGGHSTSDDPRLYRSEEEVESWG
                     KADPLLKLRRHLLWRNAWNDELESEWQVRCDTEVKDCVRRAEEKPAPELRELFRDVYA
                     EQPWHLEEQEAQSLAGPRSRDEESGS"
     gene            11797..12771
                     /locus_tag="VFG22_11855"
     CDS             11797..12771
                     /locus_tag="VFG22_11855"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HET6416983.1"
                     /translation="MTEMNLVQALNSGLRSALHEDESVLLLGEDIGRLGGVFRVTEGL
                     RREFGAERVLDTPLSETGIIGTAIGMALNGLRPVAEIQFADFIYPGFDQLVSEAAKYR
                     YRTAGEFHVPLVVRTPYGGGIRGGQYHSQSPEALFIHTPGLKVVCPSNPYDAKGLLIE
                     AIRDEDPIVFFEPKRVYRAVRMEVPEGPYSVPLGQAKVVRPGEQVTCFAWGAMLYEAF
                     AAAEEVAKQGVDTEVIDLRTLWPLDIETLVTSVQKTGRVVIVHEAPRTCGFGSELMAL
                     ITERAFVHLEAPPLRVTGWDTPFPLTHEREYLPLAHRIAPALLETARY"
     gene            12775..14019
                     /locus_tag="VFG22_11860"
     CDS             12775..14019
                     /locus_tag="VFG22_11860"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HET6416984.1"
                     /translation="MARLEFKLPDIGEGVTEGEIVNWFVAVGDELAEDDPMVEVMTDK
                     ATVIIGAPCDARVERLLFEVGAVARVGQVILTLESEAASNIDSAMRSSRPPATAVGEL
                     QDELPGARMFAARNENTPSDEAYFQPKPLAAPATRQLAKQLGLDVRRVRPTGSGGRVT
                     PDDLRAAIGNSTPEDQPGAALEERTPIVGMRRTIAERMQRAVTTAAHFTFVEECDATR
                     LVELREHLLPEAQRKGIRLTFLPFIVRAVTDALRKHPVLNSAIDERTNELVCKRYYNI
                     GVATATEAGLMVPVVRDADRLGLFELAARIQALTDAARARKLAPEELRDSTFTVTSLG
                     KQSGLLATPILNYPEVGILGVHRIKEKPVVRDGEIVVGKQMLLSLSLDHRVVDGHVGA
                     AFAYDVIASLEAPEQLFDGQLR"
     gene            14016..15044
                     /gene="mutY"
                     /locus_tag="VFG22_11865"
     CDS             14016..15044
                     /gene="mutY"
                     /locus_tag="VFG22_11865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HET6416985.1"
                     /translation="MNPIRQKLLDWYDESARDLPWRHTRDPYAIWVSEIMLQQTRVDT
                     AIPYYQRFLERFPTPEALAEAEEDAVLSHWSGLGYYRRARLLHAGVREVVSQYGGEVP
                     EDEEARRALPGIGRYTAGAIGSIAFDKEEPIVDGNVTRVLARLFRIDTPVGASTTTKR
                     LWEEAGRLVPGERPGALNQALMELGATVCAPKQPRCEACPVSRDCAALAHGEVAALPV
                     PRPRKPPRQVKLTAVIATRGRGTKKSVWLVKGKQALFGGLWGVPMSEGEAQGALREAG
                     LRARLHPEPHALIEHTLTHRRLKVEVFRASAAHGSDTPSRRLFHPEALGGVGVSSLTR
                     KILSTADL"
     gene            15108..15998
                     /locus_tag="VFG22_11870"
     CDS             15108..15998
                     /locus_tag="VFG22_11870"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6416986.1"
                     /translation="MGILAYEGVSKRFGQLSALEGVSLEVVPGEVFGLLGPNGAGKTT
                     LIRIGLDILRPDEGSVSLFGAAPSSKSLDRTAYLPEERGVYRRAKVRDLLTYLGCLKG
                     LKRRAARIAADHWLERVGLGAVANQRVESLSKGMTQKVQIAACLMTDPELCVLDEPFS
                     GLDPVNVALVTELIEERRRCGRTTVLSTHMMHQVEALCDRVALIHRGHLVVYGELDEI
                     RRRYSPHEVIVWTSMDLVAYGVPAKQHKTGGWRVELPPTATPAGYLDTLVRSGVLIER
                     YEPLVARMDEIFVNLVSESC"
     gene            16002..17270
                     /locus_tag="VFG22_11875"
     CDS             16002..17270
                     /locus_tag="VFG22_11875"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6416987.1"
                     /translation="MRFHRIAAVARFELSSVVKRWSYLIATFGLPLFLAAVSGTVLGA
                     QTYFLTQRAAESSQFGLVDEADVLDRSVFADGEKHPTWTANANEILLYGDRESATKDL
                     ESGLLRAVYVVEADYLATGEVRAIQSEKTPLLSMHGTSIEPLLRSLLRKSLLEGRLDD
                     GLQERVISPAYFVRSRLGPDGDEVAEIDEALDLLVRTTIPLFLGILLLTALLSASGYL
                     VQTVSTDKESKIVEVLLSSVTPEEILAGKLLGLGAAGLIQFAVWSSMVIFVALTASAA
                     LASVVTIPWVALAVAPIFFVLGYLFLGSLMLATAALGANAAESQKLTVGWAMLAILPL
                     MVLVILLEEPNGTLGQVMSWIPFTSPLTVIIRLAVEPAGIAWWEVLGVIAVLLGSTWL
                     SIRVGARLFRVGFLLTGSRPSLAELWRQAR"
     gene            complement(17294..17962)
                     /locus_tag="VFG22_11880"
     CDS             complement(17294..17962)
                     /locus_tag="VFG22_11880"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET6416988.1"
                     /translation="MGKTGMFRRLGVIGDVHCESETLERVLDALETMTVEAVLCVGDL
                     VDGPGNADAALAILETRGVQCVAGNHERWFLAGEQRSLENATAEIGPASRRFIEALPK
                     TRLYETENGTAMLCHGVGEDDEAWLLPDTRGYALQDISTLRELMLDESVQFMIGGHTH
                     ERMVRVFPGLTVLNAGTIHRKNEQTFMVLDFGAMRVAFHAAAEGEIGKLIEERDLPLP
                     GQVD"
     gene            18047..19369
                     /gene="serS"
                     /locus_tag="VFG22_11885"
     CDS             18047..19369
                     /gene="serS"
                     /locus_tag="VFG22_11885"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HET6416989.1"
                     /translation="MLDLRHVSENLADVKAALTRRGFADFNLLGRLGALADERRSVIT
                     GVEALRQERNEASQAMAKVADKKSDEFQAKREQLRLLGDRIKQGETRQTEVESELEAI
                     LLNLPNLPHADTPDGLSESDNVELRVWGDVPSYDFDVRDHVEIGTRLGILDFERAAKI
                     SGSRFVVLRGLGSRLNRALMQFMLDVHTDEHGYQEVWPPVLVKDSAMLGTSQLPKFAK
                     DAFRMAKDAEWEAEAEAEGHDLYLVPTAEVPITNLHAGEILSADELPLAYTGYTACFR
                     SEAGSYGKDTRGMIRVHQFDKVELVRFCEPEDGASQLELLTEHAETILRKLGLHHRVV
                     QLCAGDMGFAAQKSYDIEVWLPAQNAYREISSCSWFGDFQARRMKARFRPADKGKPQF
                     LHTLNGSGLAIGRTLVAILEQNQQADGSVIVPEALRPYMGGTERIPAP"
     gene            19379..>19701
                     /locus_tag="VFG22_11890"
     CDS             19379..>19701
                     /locus_tag="VFG22_11890"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET6416990.1"
                     /translation="MSMLEIQRDVPLAPLTTFGLGGPAKYFARVDDEATLLDALRWAA
                     DEGLRSAILGGGSNLIVPDEGYPGLVVQMALGDLEFLTGGCVIAGAGVPWKAVVDGAI
                     SRDWAG"
BASE COUNT         3827 a         6151 c         6133 g         3590 t
ORIGIN      
        1 gacggcgtct ctcggcccga gtgtctccct cggtcgactg ctgcgatgtg ggttccagca
       61 gctgtgctga aatgggcatg gccaccgagc tgagaagcgc gagcaagagg actgagcaac
      121 gaagcctaga catcagagca cccacctccg ctcagccaag acccggccca atacgcctcg
      181 aagatgaacc gcgatgacaa acgatccttc acgctcctcg agcaaaccga ggtgcgccat
      241 ccggctgagc tgcgcctgcg cggtattgac atcgagcctg aagaggcgtg cctggccggc
      301 tgcgtccatc catccttggc gcgcgatctg gtagaggttg accaaaatgt cgtccgggag
      361 acgcctcaca cgcgcgtacg aggaagccgg tatcggtccc acatgcacga tgtcgtcctg
      421 aatgccgcgg atcgaggcca accagagccg caaggcatcg cgcacgagtc ctccggtcgc
      481 cgcgtgaagc tcgtggaagt actgatcgta cgggcgacga atacggcttg cgcttcgagc
      541 gaataaacct tcgatcggag aaccgtcacc tcggtcgaag gagtgttcaa atccgctcag
      601 tcggtgccgt gcgatgaccg cagcctgaag cgcttcctgg tccaagggct cgagccgcac
      661 gatctctgga aacgcgtcgc ggaggggcgc caccgtcgaa gcaaagttcc agaagagaac
      721 cgcaccatgc gtcaaccagc gccttcgccc accctcttcg atgatgcctt tgacgaaacg
      781 acggagcgga tcaaagcctc caggcgccat cgacagcatc cagtgcaagc catccaccac
      841 caccagctga ccctcgggcg cgtcctggaa gatcgcgtcc acatcctcca gggaaaccgg
      901 ggccgtgaat actacacgtt tcacgttctt ccaacgtcga ctccgaacga ttgcgctcga
      961 cacggccgcc ttacctacgc catccacgcc caccagcgcg acggaccgta gccgtcccct
     1021 gatctcgctc gagagcacgg actcggcgcg acggatctcc tcatcccgcc ccgtcagcac
     1081 gtccccggcc tccatggtgt cagctgcaaa aagccgccgg tagacgagcg gaagatcggc
     1141 cacgacctca gccgccgcga aggtccctgc tgagagcgca tcgctcggca aggtggccgt
     1201 caatcccagc cgctcgctcc atcgccccag gcgctcttcg ccgaagatgg cacgaaggcc
     1261 gccgcgaaac tgggacttga gccgcttcat tcgaggcagc cgctcggcct tctcgatcat
     1321 gcggagaccg ctcgcggcac gccgcatggc gtctagtcgc gacttgctga actcgccttc
     1381 tgcgagctcg ccacgaagtg tttggagagt cccgagcgac gcatcgcgca tctctctttc
     1441 gatcatggca ggccagcctt gcgcctccgc gcggatctcc tctaatgagt tgtagcttcg
     1501 ctcgagctgc ccgtagacca tgtcgcgcaa cagcgtgtgc atctcctcgg ggatctcttc
     1561 atcgtgcacg atctcgagct cggcggttgc gatctcgacg ttgaaagcga ccacgcgctc
     1621 gagctcttgc aatagcccgg acagaggttg gacgcgttcc gcgagctccc tacggctggc
     1681 gagcagcttc ggcgcgaccc gactgtcgat gtacgtgaga accacttcgc ggaacggcac
     1741 ctcgacctcg ggaagggccg tcggaagtcg ccattcaccg gtttgacgtt ggcccacggt
     1801 aacccggtag cgcggcgtga gaccacggca ggcgtccacc agggtgtcga gcagaggggc
     1861 gaccttcgag tcgtcgctca ggtcctggta gagctcggtg gtcacgcgtg cggcttcacc
     1921 cgtgattttg ctggtcggct ccgtcgattc ccgcaggatc accgcgagct cgtcgcccga
     1981 atagttggat tcgagctccg tgtcgagctc ctcgagtgct ttgtgcaggg tctcgctcac
     2041 gcggtcgacc tgcaaggtcg cgcggcgctt gaccgtgttg gtcagccgca cgacgtattc
     2101 ggcgacgtga tccttgatgc gcgcttcgag gccgagcagc tcgagctcca tcgccagtga
     2161 ggagtattcg gcagcgctgg cttttcgaag gttcgagacg tccttcgtga gggtctccat
     2221 ggcacgcatt cggttggcaa accgtctgct ggtgcggcgc tcgctcgcgg gcagatcgag
     2281 ggtcccgaac gtcggaagat cttctttgat gcggacgaag cccgtcgcca gggcactggc
     2341 cgcgcgatgc gtaccatcgc cggtgatgcg cacggtctcg tccagcgcga gagcgagctc
     2401 ttcttcgacc tcgtgccgca accgaacgag ctgtccttcg aggtctgccc ctgagtcgct
     2461 cgagatttcg acgatgtcgt catagcccgc gatgaccccg tcgaagagac tgcgagttct
     2521 ggcggcgagg tgtcgatcgg cttcgacgaa cagggctgcc agaccttcga ggcgcaccgg
     2581 cgcgtcgtag gagaggtgat agcgtccgag cgcccgcaag tgaacgtcgc gagtcggcgc
     2641 ggcttgcccg aatgtcttcc gccagctgcg cctgaggcga aggccctggc gacggacgct
     2701 cttccaaaga gaatcgtctt tcttcgattc gtaggacagc tcttcgaacg gcgccgcgac
     2761 gacttcgggc agctcgtcga ccagcgcgtc caaattgtcg agaatcgatt ccggagtcca
     2821 ggtcagcttg gcgagggtca ccgagcgacc aagcacgatc cctctccagt gggcggcaag
     2881 atcagattgc gcggaacgaa gcatcgtcac tagctcgtcg cgctctgcgc cctcttcggc
     2941 gcgggcctgc accgtcaagc gacgatgatg tcgaagaaat tcccgtcgca agtcccttag
     3001 atattttcca gcttccatct ggaactctcg gagaggaccg gtcgacactt cgcgaacgat
     3061 gctctgcagg tcgcccgcga gctcctgagt ccgactgttc agctctgcac tcgaagtctc
     3121 gagcggcaga ccccagaggt tcttttcacc gacgtgctcg ggccatggct ccagctgttt
     3181 cgccgctccc gcatcgtccg ggagcatgga gggaggcttc accgatagat gtcgggaggg
     3241 tgccatcgac ttccggtggc tgctcgtctc accggccagg ctgaggccca gcttgaacaa
     3301 gaccggtccg atcagctcgt tgatggccac gccgccgatg attagagttt ccagtgagcg
     3361 cccgagctct ccgaacctgt tgcccacgat cgcagccagc aggatggaga ccccagcttg
     3421 tgagacgaag cccatccatc cgaacgcttg ggtcgcttca tctgccttgc cgaaccgggc
     3481 gccgaccttg gtgcctgaga acatcgcaaa cccgcggacg gtgaccagac ccagggcgaa
     3541 aagggcgacc tgccggagct cgtcgagatg gagctttgcg ccggccagcg tgaagaagac
     3601 gacgtacacc ggaagtgaca gtcgctcgac cgtctcgatc agccggtgcc ccgtcttcga
     3661 gaaattcgaa acgccgaagc cggctgcgag gaacacgagc acgaggtccc agtcgagctg
     3721 cttcgaaatg aaggcgacca agtacacgac gcccaccacg aagatcagca gctcttgatt
     3781 gacgtagcgg atgtagaggg ccatcaagcc cccgacgacg accgcgccga agagaatcga
     3841 gatgacgatg tgctggagga ggtagctcgc gatcccgcct tcgagtgcgc caatgccgat
     3901 ctgttgggcg acgaccactt gggcgactgc aaaggccacc acgacgatca cgtcttttag
     3961 gacgacgacc gaaaggacgt tcttcgtcat cataccgatc gcacgcgtct cggtgatcac
     4021 cgcgatcgta gcggcaggag aggttgccag agacaccgat gccaccatag cgcctacggc
     4081 aagcgcagcg gccatcggca accccgcgac ccctggcagc ccgacgtacg gcacggcccc
     4141 cgagatcagc cagaagaacg ccgtgacgag aaccccaacc gagacgattt gcgcagccag
     4201 gatcgagctt atcgcgcgaa atcccttctt gaggccttct agcttgagct ctccgccggc
     4261 cgtcaaggcg atcagcgcca cggccaaggt gtcgaacagg ctgagttgtt cgatcacctc
     4321 ttcattgaga atgcccttgt cgaacggcgc gggtaaatcg aacccagtga gcgccttcgc
     4381 caaagacggt ccgaacacca aacccgcgat gaggtagccg gtgatgtgcg gcagacggat
     4441 ctggccgacg agcccgccga tcgtgtagct cgcgagcacc acgaagccga acgcgagcat
     4501 gccgctcggg tcgaacgtgt gctgggcgaa ttgttcgaac tgatagagcg ccaccatgcc
     4561 gaacagcagc agcacgatca ctgtgatctg tcttcgcgcg tgtgtctctt cgccgggcgg
     4621 tggcatgctg taggagccag ccatcagcgt ctccagaacc cgggcaggat caaggcgaac
     4681 aaggtgaaga actcgaggcg gcccagcagc atggccaacg agaagaacat cttactgccg
     4741 gccgaataac tcgcgaagtt gtcgggcccg acgtggaagg gcgccggccc catgttggat
     4801 aagcaggtga gcatggcgcc gaaggccgta ggaatgggga ccccttctac caaagtgacg
     4861 aagagcagtc cgaacgcgat gcatcccatg tagatcaaga agaacgacgc gacgtcggcg
     4921 agcacctgcg tactgatcgc tgccttgccc atccgaacca actccaccgt ggatgggcga
     4981 aagctcttct gaacctgggc aatcgcctgc tttcccatca gcacggctcg ctcgactttg
     5041 atgccgccgg cggtcgatcc ggagcaaccg ccgacgaaca tgatgaggac cacgaccgcg
     5101 agtgcggtcg agctgtatgc gctgtagtcg tccgtgccgt agccggtcga agagatcgtc
     5161 gtggccacca tgaagaacga gtaccggaag gacgtcagca actcgtcgtg gacgctgagg
     5221 ttgaggagag tcagcacgat aaccgagaag ccgacgatca ggaggtacca gcgaaactcg
     5281 atgttgcgga agagagtttt ccagctgccg gtgcgcaaga gcccataata aagaccgtag
     5341 ttcacgctgc cgatgagcat gaagcaggcg atcacgtact cgatcgctgc gctgttgaag
     5401 cccccgaccg agctgtcgag tgtagagaaa ccacccgtag acatcgtggt gaacgcgtgg
     5461 cagacggcgt cgaagagatc gagcccaaga atcttgagca acgcgatctc gaggatcgtg
     5521 aaggcggcgt aaagcttcca tagagtgaac gaggtttcgg cgatgcgcgg cctcaagccc
     5581 tctgcagtcg ttccgggtac ttcgccgcga aagaggtgct tgcctccggc acccaggttc
     5641 ggaaagaccg cgacgaacaa gacgacgata cccattccgc ccagccattg aatcagcgat
     5701 cgccagagca agaggggacg cgaaagacgc ccctcgatgt ccgagatcac cgtcgcaccg
     5761 gtggtggtga gcccgctgac ggactcgaaa aacgagtcgg ccggagacat gccggctcca
     5821 agcatgaacg gaaccgctcc gcaggcgccg gcagcgatcc agatgaggct caccgccaga
     5881 agtgcttcgc gacgggatat gcgctccgag acgaagcgat ttccgtaaag gaatgcggcc
     5941 aagccgacga tcgacacgat gatcgcggaa agcgcgagcg atgcagctcc accctgggga
     6001 cgcgtggggt cgggcgcgaa ctggtcccag accattccga ctcccgcgca caaggcgatc
     6061 atcacaccga tgccgagaac cacggctccg accggtcgaa tcactccacg aaaatctctg
     6121 gactgggtgc tcactcgccc ctcggtcgaa acagtcgctc gacgctcgca cgggcttcct
     6181 tggtggtcat caaaatcgcc ctgtcgccgg cttgaacgat gtctttgcca tgcggaatga
     6241 tcacgtcgtc tccgtggacg atcgccgcca gcagggagcc cctcggcagg ttcattcggt
     6301 gcagaggctt gttgaccgct cggcactttg gactgacggt gagctcgacg acctcggctt
     6361 ggccgtcttc gagcaccgtg aggttgtgga ggcccccgcc acgcgaaaaa cgaaggattt
     6421 ggtccgacgc gacggtgcgc ggcgagagca cgatgtctat tccgagctga cgatagatcg
     6481 aagtgtagtc cgcgcgttgg acgatcgtgg ccgtgcgctt gcagccggca cgctgtgcca
     6541 acaaagctgc catcagattg atctcgtctt gtcgcgtgac cgcggcgaag aggtcgtagg
     6601 tgccgacttc ttgctcttcg agcaaggcgc ggtccgtgcc gtcaccgtgt accacttcga
     6661 caccttgaaa ctctgcgctg atttcttctg cggacgccag gtcgctctcg atcatcatca
     6721 cggtggcttg ctgctgcaag agctcccgcg ctagcgcctt tccaacgacg ccgcctccaa
     6781 cgatgcagac gcgacgcgcc ttgcgctcct tgctgaagag ccgctcggcg gcgagcacac
     6841 cttcaggacg tccgatcagg tacactcgat cgcctttgac caacacgtca gcgccgccag
     6901 ggacgaagag ctccccatct cgaacgaccg cgctcaccag ggtgtgagcc ggcagctcga
     6961 cggaggagag cggcttgttg aggacacgag attcgccgag gtcgatttgg acgagctcga
     7021 cgcgatcctg cgcgaggtct acgacgtccg aggcgccgta agaccgagcg atgcgactga
     7081 gctcctgagc caccaggaca cgcgggttga tcaccacgtc gaccccgagc aggccgtatc
     7141 gaatcccctc ggtccattca gaccaagcgg ttccctgtac gcgcgccacc acgtgtttcg
     7201 ctccgagctg ctttgaagct agcgcagcga tgaggttcat ctcgtcgtgg ttggtcactg
     7261 cagccacgag gtcggcgcgt ccaacgccgg cgattttgag tagtgtttcg ctcgccccgt
     7321 atcccacaat cgatgccacg tcgtagtgct cttcggcgta ctgcaccgcg ctggcgctcg
     7381 aatcgaccgc aatgatgtcg tgtccttcct tgtcgaggac gctcaacaag tgacggccga
     7441 cttctcccaa acctatgatg acgatgttca tctcgcagct acctcgaccg ttgtttggga
     7501 ctcgggttcc ttcagctctc tttgcagctc gccctcgtcg accaagagtc ctcgcagcaa
     7561 tcgtggcgcg atcagatcgt tgaggaccac ggaggcgagc acgacggtgt acgcgatctt
     7621 ggccgcgtcg ccttcgtaga ccagacgaaa agagaccgcc atagccagcg tcacgtcgcc
     7681 gtgagcgagc agtcctcgaa acaggtcctt gcgaaggctg gttccccaac ctgcgatgac
     7741 gctcgcgagc catttggcgc cggtgcgagc gatcaaaaag ccgccgagac caagtaaggt
     7801 gggaaccagc ggggtgggtt gccaaagagc gcccgcgaag atcagtagga cgatcgccat
     7861 cggacgctcc gtcgattcga gcgtcgagtg aagcagtggg ccgccacgtg cgaagttgac
     7921 cagcacgaag cccatcgaaa ggttcacggc aagaggcgaa agctcgagaa agtacgcgcc
     7981 gcccgatgcg aaagcgatga tccccaccag cgccaggaaa cgcgaattgt cggattcatc
     8041 gccggccaaa aagagcgaga acagaaagcc tagcgcagcc ccaagcagga cggtcaccca
     8101 tgcccacaaa gccggcgaat agtcctgcgc ctcgggactg ttttcgtgga agacgcagaa
     8161 gatcaatccg aatgcgagaa tgacgaggat gtcaccaaga cgagtgccgt ttcgcagaag
     8221 accgaccagt cgtccgctca tctcgtagcg cctggcgagt aggtcgaaag gttccgcgga
     8281 gtcggcagcc gcaaaacagg ctagcgcggc tgcactgagt gcagcatcgc gcggcgtcgc
     8341 tgcaacccat ccggactcgg tgaagaagta ataggctcca accccaacgg cgacgccggg
     8401 aatcagatga tgaagaatca ccactctcca ggtcgcggga tcgagccttt gaagactcgc
     8461 aaagtcgaag tccgttcccc tcaagagacc gacccagccc gcggcgagcg cgatgatagg
     8521 cagtaagccc gcgatgttgt cgagcgcatg gatggccggg aaagccggtc cgagcagaaa
     8581 gccgagcaac aaatactcgg caccgctcac gacgaggaaa cggtgctgta ggcgctcgac
     8641 gacgttgtgt gcgagcacgt acgcgacacc gatcaccaat acgagcatga cgatcgtacc
     8701 gagctcgccc gcgccgccct ctgcacccga ttcgctcgca gcgcgcacga cgcccggcga
     8761 tgtcgcccaa aggagcgaca gagccgccaa aatcagcctc cccgccatat ccgttcaatt
     8821 gtgaagaaga agcccgagga gcggtagttt caggctggac aggtgtttag ccccgcaggc
     8881 cttcgagcac gatcgagatg agaccctttg tggtttcgac gggatccaac tgaatctgat
     8941 tgcgcaccga agccagcgca agcgcctcca cggcacccag cacggccagc gcgctcacac
     9001 ggggatcgga aatcgacacg aacttgcggt ccacggcggt ttggctgagc gtcatcgcac
     9061 gattgtcgag atcgtttgcc aggtcagata tcgcctgtcg cgcggtcgtc gcaggggcgc
     9121 gtctttcttg aagatacagg cacatggcat ctgggtgcat ggccagcgcg ccggcaagcc
     9181 tgttgccgag ctgcgagtag accgcgacgg cctcgtcacg agagttgact tcgcgcagct
     9241 tggtctcgca ttcttgaatg gcatcgagca tgacactggc caccggctcg atcactgcct
     9301 cgacgagatc ggctttgtcg cggaaatacc ggtaaaagtt gccctttgcc attcccgctt
     9361 cacgagcaac ttcgtcgatc gtgacggctt ctatgccgcg ggcgaggaat agcgtgagac
     9421 ccgcgtcgac caggtcctgg acgcgcttgc gccggttgat gtctctttta ccaccatgtg
     9481 caccgggtcg ttccaccggc ccagcgcgcc tggggcgtcg ttcctcgtcc atcgctttgc
     9541 tcctaacggg ggaatgagct gctgcgaaac gtcattatgg ggcacgactg cggcacgtaa
     9601 agtaagtttt gcgaaaatat tgcgcccgag ggttccccac atcgaaagct tgcggaaaac
     9661 ccaagaaacc aacacaattc gtgacgttag acgcgattga cagagggggt cgcgactcct
     9721 aagagcttag cctaggaccc cgggagtatt tggatgagct tgccagcccg aaaacctgat
     9781 tggctacggg tgagacttcc ggcgggaccg gagcaggacg aattccagag cctgaaggcg
     9841 aagttccgct ctcgaggcct acatacggtc tgcgaagagg cccgctgccc aaacatcttc
     9901 gaatgctggg ggcaaggcac cgctacggtg atgatcctcg gagaaacctg cactcgcggc
     9961 tgcaggttct gctccgtcaa gacgggaaag cccgggggaa tcgtggaccc tcaggaaccc
    10021 gagaacaccg ccctctcgct tgccgagatg ggtcttgcct atgtggtcct caccatggtc
    10081 gatcgcgacg acctcgatga tggcggcgcc gcgcacgtca gcgagaccgt caggcggatc
    10141 aaacaacata gccccgatct tcgcgtcgaa acactggttg gcgactttct cggcaagcga
    10201 gatgacgttc ggacgctcgt gcaagaaggg cgccccgacg ttttcgcgca caacgtcgag
    10261 gtggtaccgc gcttgcagcg gacgatcagg gacgcccgct gttcctggga ccgttccatc
    10321 gacacgctcg cggcggccaa ggagctcggc tccgccgtta cgaagtcatc cctcatggtg
    10381 ggcctgggcg agcagggcga cgaagtcctc gaagcgatga ggtttttgcg agccgccgac
    10441 gtggacgtac tcaccatcgg tcaatatcta cggccgacca aacgtcatgc acccgtgcag
    10501 cgctacgtgg atccggatga gttcctcgat tacgaacggg ccggcctcga gatgggcttt
    10561 cgctacgtgg cctcggggcc actcgttcgc tcgagctacc gcgccgcgga ggctttcctc
    10621 catggtttgg cttcggagaa agccaccgcg ggcgagaggc ccccaatgaa tcgctacggc
    10681 aggaaacgga agctctcggt gctcggctga ggcaaggatg caggtcattc gagacgacgg
    10741 aagcctcgac ccgcaactcg acccgggcct ctcgatcgac catgtggtcg cgctttacaa
    10801 ggcgatggtc cggacccgcg tcatcgacga ccgcatggaa aaaattcagc gtcaggggcg
    10861 catcgcgttt cacgtcggct ccctcggcga agaggctgca atcctcggtg cggtctcggc
    10921 gatgcgcgac caggattgga tcgttccgtg ctaccgcgaa gtcggcgcgc ttctatggcg
    10981 cggatatcca cttcagaagt acgtcgatca catgtacgga aacgctggtg acccagtgca
    11041 cggccgtcaa atgccggacc acagcttcag ccgcgcgcac cgctatctgt ccgtgagcgc
    11101 gcccatcgga actcagattc cccatgccgt gggcttggcg atggcagcca agaagcgcgg
    11161 tctcgacgag agcgctgcgg tgttctttgg cgacggagcg acgagctcga acgacttcca
    11221 cgcggcgatg aacttcgcag ccgtgtatca agcgccggtc ttgttcttgt gccgaaacaa
    11281 cgggtacgcg atcagcctgc cggtagcgca acagaccgcc actcggactc tggccgaaaa
    11341 ggcggccgcg tacggcgtcc cggctgctcg cgtcgatgga aacgatgcgc ttgccgtttg
    11401 gggcgccgta cgacgaggcg cagagcgtgc ggcagaagac ggcggtccga cattcatcga
    11461 gatgatgacc tatcgtcggg gcggccactc gacatcggat gacccgcggc tctaccggag
    11521 cgaagaggaa gtcgagtctt ggggcaaagc agaccctttg ctgaagctcc ggaggcacct
    11581 cctgtggcga aacgcttgga acgacgagct cgaatccgag tggcaggtca gatgcgacac
    11641 ggaggtcaag gactgcgtgc gtcgggccga agagaagcca gcgcccgagc tccgagaatt
    11701 gtttcgggat gtttacgccg agcagccctg gcacctcgag gaacaggagg cgcagagcct
    11761 cgccgggccg aggtcgcgcg acgaggagag cgggtcgtga ccgagatgaa cctggtccag
    11821 gccttgaaca gtgggcttcg ttcggcgctt cacgaggatg agagcgtgct cttgctcggt
    11881 gaggacatcg gacgattggg cggcgtcttc cgagtcaccg aagggctgcg ccgcgagttt
    11941 ggtgcggagc gggttctcga tacgccgctc tccgaaaccg gaatcatcgg aaccgcgatc
    12001 ggaatggcgc tcaacgggct gaggcccgtc gcggagatcc agttcgcgga cttcatctac
    12061 ccgggtttcg accagctggt gagcgaggcg gccaagtacc gctaccgcac cgcgggcgag
    12121 ttccatgtgc ccctggtcgt gcgcactccg tacggtggag ggattcgcgg tgggcagtat
    12181 cactcgcagt cccccgaggc gctcttcatt cacacccctg gcttgaaggt ggtctgcccc
    12241 tccaacccgt acgacgcaaa aggacttctg atcgaagcga ttcgcgacga ggatccgatc
    12301 gtttttttcg agccgaagcg cgtctaccgc gccgtgagga tggaagttcc agaagggccg
    12361 tacagcgtgc ccctcgggca ggcgaaggtc gtgcggcccg gagagcaggt cacttgtttc
    12421 gcctggggag cgatgctcta cgaggcgttc gccgccgcag aagaggtcgc caagcagggc
    12481 gtcgacaccg aggtgatcga ccttcgaacc ctctggccgc tcgacatcga gaccttggtc
    12541 acgagcgtac agaagacagg ccgcgtcgtg atcgttcatg aagccccacg aacctgcggc
    12601 tttggtagcg agctgatggc gctgatcacc gaacgcgcgt tcgtgcacct cgaggcgccg
    12661 ccgctccgtg tgacaggctg ggatacgcca tttcctctca cccatgagcg tgagtatctt
    12721 ccccttgcgc accgtatcgc gccggctctg ctcgaaacgg ctaggtactg agcgatggct
    12781 cgactcgaat tcaagcttcc cgacattgga gaaggcgtga ctgaggggga gatcgtcaac
    12841 tggttcgtcg ccgttggcga cgagctggcc gaggacgacc cgatggtcga ggtcatgacc
    12901 gacaaggcga ccgtgatcat cggcgccccc tgtgatgctc gcgtggagcg acttctattc
    12961 gaagtgggcg ccgtggcacg cgtagggcag gtgatcctca cactcgagtc cgaggcggct
    13021 tcgaacatcg actccgccat gcgatcttcg aggcccccgg ccactgccgt cggtgagctc
    13081 caggacgagc tgcccggggc gcgcatgttc gcagctcgca acgagaacac gcccagcgat
    13141 gaggcttatt tccaacccaa accgcttgcg gccccggcga cacggcaact ggcgaagcag
    13201 ctcggcttag acgttcggcg ggtccgtccg acgggctccg gaggtcgtgt cactcccgac
    13261 gacttgcgcg cggcgatcgg caactccaca ccggaggatc agccaggtgc tgcgctggag
    13321 gaaagaacgc cgatcgttgg catgcgcaga acaattgcag agcgcatgca gcgagcggtc
    13381 accaccgccg ctcacttcac cttcgtcgag gagtgcgatg ccacacgcct ggtcgagctc
    13441 cgcgaacatt tgctccccga agcgcagcga aaggggattc gactcacgtt cttgccattc
    13501 atcgtcagag cagtgaccga tgcgctgcgg aaacaccctg tcctcaactc ggcgatcgac
    13561 gagcggacca acgagctcgt ctgtaagagg tattacaaca tcggtgtggc gaccgcgacc
    13621 gaggctgggc tcatggttcc cgtcgttcga gatgcggacc gcctcggtct cttcgagctt
    13681 gcggctcgaa ttcaagctct gacggatgcc gctcgtgcgc ggaagcttgc gccagaggag
    13741 ctgcgcgact ctacgttcac ggtcacctcg ctcggaaagc agagcggcct actggcgacg
    13801 ccgattctca attatccgga ggttggcata ctcggtgttc accgaatcaa ggaaaagccc
    13861 gtcgtccgag acggcgagat cgtcgttggt aagcagatgc tgctttcgct ttcgctcgat
    13921 catcgagtcg tcgatgggca cgtgggagcc gcgttcgcat acgacgtcat cgcttcgctc
    13981 gaggcacccg aacagttgtt cgacggacag cttcgatgaa tccgatccgt cagaagctgc
    14041 tcgattggta cgatgaatcc gcccgtgacc tcccttggcg tcacactcgc gacccgtacg
    14101 cgatttgggt gtcggagatc atgctccagc agacgcgcgt cgacaccgcc atcccatact
    14161 accaaagatt cttggagcga ttcccgacgc cggaggcttt ggcagaggcc gaggaggacg
    14221 cggtgctctc gcattggagt gggcttggct actatcggcg cgctcgcttg cttcacgccg
    14281 gcgtgcgcga ggtggtgtcc caatacggcg gagaagtgcc cgaggacgaa gaagcacggc
    14341 gtgcgcttcc agggatcgga cgctacacgg ctggagcgat cgggagcatc gccttcgaca
    14401 aggaagagcc gattgtcgat gggaacgtca cgcgagtgct cgcacgcttg tttcggatcg
    14461 acacgccggt tggcgcctcg accacgacca agcgcttgtg ggaggaagcc gggcgtctgg
    14521 ttcccggtga acggcccggc gcactcaacc aagctctgat ggagctcggc gcgacggtat
    14581 gcgcccccaa acagcctcgc tgcgaggcct gcccggtttc gagagattgc gctgcacttg
    14641 cccatggtga agttgccgcg ctgccggttc cgcgtccgcg caagccgcca cgtcaggtca
    14701 agctcacggc ggtcatcgcc acccgaggtc gggggacaaa gaagagcgtc tggctcgtca
    14761 aaggcaagca ggctctcttc ggaggcttgt ggggtgtacc gatgtccgag ggagaagcgc
    14821 agggcgctct tcgtgaagcc gggctccggg cgcgtcttca tccggagcct catgcgctga
    14881 tcgagcacac gctcacgcac cgccgtttga aggtcgaggt atttcgggca tcggccgctc
    14941 atggaagcga cacgccgagc cgccgtctct ttcatccaga agcgctcggg ggcgtgggtg
    15001 tgtcttcgct cacccgaaag atcctgagca cggcagatct ctagacagcg tcgggctcgg
    15061 ggccccggca tttggtcgct tcacccttct gccgatacac tctgcaggtg gggatcctcg
    15121 cgtacgaagg cgtgtccaag cgcttcggcc agctgagcgc tctcgagggc gtttcgctcg
    15181 aggtcgtgcc cggtgaggta ttcggcctac ttgggccaaa tggcgcgggc aagaccacgc
    15241 tgatccggat tgggctggac atcctccgtc ccgacgaggg cagcgtgtcg ctctttgggg
    15301 cagcaccgag ctcgaagtcc ctcgatcgga ccgcatacct tcctgaagag cgtggagtct
    15361 accgccgcgc caaggttcgt gacttgctca cgtatctcgg ctgtctgaag ggcctgaagc
    15421 gaagggcggc tcggattgcg gcggatcatt ggctcgaacg cgtgggtttg ggagcggtcg
    15481 ccaaccagcg cgtcgagagc ctgtcgaaag ggatgacaca gaaggtccaa atcgcggcct
    15541 gcctgatgac cgatccggag ctctgcgtgc tcgatgagcc tttctcggga ctcgacccgg
    15601 tcaacgtggc gctggtcacc gagctgatag aggagcgccg ccgctgcggt cgaacgaccg
    15661 tcttgtcgac ccacatgatg caccaggtgg aggctctttg tgaccgggtg gctctgatcc
    15721 accgcggcca cctggtcgtg tacggagagt tggacgagat ccgcagacgg tattcgccgc
    15781 acgaggtgat cgtctggacc agcatggatc tagtcgccta tggcgtgccc gccaagcaac
    15841 acaagaccgg aggctggcgc gtggagcttc ctccgaccgc aacccctgcc ggctacctcg
    15901 acacattggt gcggagtggg gtgttgatcg agcgctacga gcctttggtc gcacggatgg
    15961 atgagatctt cgtcaacctc gtgagtgaga gctgctgacg gatgcgcttt caccgcattg
    16021 cagccgtggc gcgcttcgag ctctcatccg tcgtcaagcg ttggtcgtac ttgatcgcga
    16081 cctttggtct tccgctcttt ctcgcggccg tttcgggaac ggttctcggc gcacaaacat
    16141 acttcctcac tcagcgcgcc gcggagagct cccagttcgg actcgtcgac gaagccgatg
    16201 tcctcgacag atctgtattc gcggatggtg aaaagcaccc gacctggaca gccaacgcca
    16261 acgagatcct gctttatgga gaccgtgaga gcgcgaccaa agacctcgag tctggcctcc
    16321 ttcgcgctgt gtatgtcgtg gaagccgact acctcgcgac cggagaagtt agggccattc
    16381 agtctgagaa gacgccccta ctcagcatgc acggtacctc gatcgagcct ttgctccgtt
    16441 ctctcttgag gaagtcgtta ctcgaaggcc gcctcgacga cggattgcag gagcgcgtga
    16501 tctccccggc ctatttcgtg agaagccggc tcggccccga tggagacgag gtcgccgaga
    16561 tcgatgaggc gctcgacctc ctcgtgcgca cgacgattcc gctcttcctc ggaatccttc
    16621 tactcacggc cctgctgagc gcgtcgggtt acttggtcca gacggtttcg accgacaagg
    16681 agagcaagat cgtcgaagtg ctcctctcct cagtgactcc ggaagagatt ctcgcaggaa
    16741 agctcctggg cctcggcgct gccggactca ttcagttcgc ggtctggtcc tcgatggtga
    16801 tcttcgtagc gctcacggca tctgctgcgc ttgcctcggt cgtcacgatt ccctgggttg
    16861 cgctggccgt cgcgcccatc ttcttcgtcc tcggctatct cttcctcgga agtctcatgc
    16921 tggccaccgc cgccctcggt gccaacgcgg cggaaagcca gaagctcacg gtgggctggg
    16981 ccatgctcgc gatcctcccg ctgatggtgc tcgtcattct cctggaggag cccaacggga
    17041 ccctcggcca agtcatgtcc tggatcccat tcacctctcc gctcaccgtg atcatccggc
    17101 ttgcggtcga acccgcgggt atcgcctggt gggaagtgct cggtgtgatc gccgtcttgc
    17161 ttggttcgac ctggttgtcg atccgcgtgg gcgcccgcct cttccgagtc ggatttctcc
    17221 tgaccggctc gcgaccttca cttgcagagc tctggcgcca ggcccgctga ggcggaaagg
    17281 gctcgagcgc tgctcaatcg acttggccgg ggagcggcag gtcgcgctct tcgatcagct
    17341 tcccgatctc gccttcggcc gccgcatgga atgcgacgcg catcgccccg aaatcgagca
    17401 ccatgaacgt ttgctcgttc ttgcggtgga tggtgcccgc attgaggacg gtgaggcccg
    17461 ggaacaccct gaccatgcgt tcgtgtgtgt ggccgccgat catgaactga acgctctcgt
    17521 cgagcatcag ctcccggagc gtggagatgt cctggagggc gtagcctcgg gtgtcaggca
    17581 gaagccaggc ttcgtcgtcc tcaccaactc cgtggcagag cattgccgtt ccgttttcgg
    17641 tttcgtagag ccgggtcttt ggtagcgctt cgatgaacct gcgagacgcg ggaccgatct
    17701 cggctgtcgc gttctccaag gatctctgct cgcctgcgag aaaccaacgt tcatggttgc
    17761 cagcaacgca ttgcaccccg cgcgtctcga ggatggcgag tgccgcatcg gcgttccccg
    17821 ggccgtcgac cagatcgccg acacaaagca cggcttcgac ggtcatcgtc tccagcgcat
    17881 cgaggactcg ctcgagtgtc tccgattcgc agtgaacgtc gccgatgacc cccaatcggc
    17941 ggaacatgcc cgtcttgccc acgacttcaa cttaggagct tttcgcgccc cgcgccaaga
    18001 accgtgcgac tggtgctcgc ccccctcctg ttgtagaccc gcttccatgc tcgacctgcg
    18061 ccacgtgagc gagaacctcg ccgatgtgaa ggcggccttg acccgccgcg gtttcgcgga
    18121 cttcaacctt ctcggccggc ttggcgctct ggccgatgag cgtcgctccg tcatcacggg
    18181 cgtcgaagcg ttgcgacagg agcggaacga agcgagccaa gccatggcga aagtcgccga
    18241 caagaagtcg gatgagtttc aggccaagcg cgagcagctt cgcttgctcg gcgatcgaat
    18301 caaacagggg gagacacgac agaccgaggt ggagtccgag ctcgaggcga ttctcctgaa
    18361 cctaccgaat ttgccgcacg cagatacgcc cgacgggttg agcgaaagcg acaacgtcga
    18421 gttgcgcgtc tggggggacg tcccttcgta cgacttcgac gtgcgcgatc acgtcgagat
    18481 cggcacgcgg ctcggtatcc tcgatttcga acgggccgcg aagatcagcg gctctcgctt
    18541 cgtggtgctc agaggtctcg gctcgcggct caatcgtgcg ctcatgcagt tcatgctcga
    18601 cgtgcacacc gacgagcacg gctatcaaga ggtctggcca cccgttctcg tcaaagacag
    18661 cgcaatgctg gggacgagtc agctgccgaa attcgcaaag gacgcttttc gcatggccaa
    18721 agatgcggaa tgggaagccg aggcggaggc tgaagggcat gatctctatc tcgtccccac
    18781 cgccgaggtg cccatcacca atctgcatgc aggggagatc ctttctgccg atgagttgcc
    18841 gcttgcctac acgggttaca ccgcctgttt cagaagcgaa gcgggcagct atggcaagga
    18901 cacgcgcggc atgatccgtg tccatcaatt cgacaaagtg gagctcgtcc gattctgtga
    18961 acccgaagac ggcgccagtc agctggagct cctcaccgag cacgccgaga ccattttacg
    19021 caaactgggc cttcaccacc gcgtcgtgca gctttgcgcg ggcgacatgg gatttgcggc
    19081 acagaagtct tatgacatcg aggtctggct ccccgcacaa aacgcttacc gcgagatctc
    19141 gagctgctcc tggttcgggg actttcaggc gcgccggatg aaagcgcgtt tccggcccgc
    19201 ggacaagggc aagccccagt tcttgcatac tctcaacggt tccggactgg cgatcggccg
    19261 cacactcgtc gcgatcctcg agcagaatca gcaggccgat ggaagtgtca tcgtacccga
    19321 ggcactgaga ccctatatgg gcggcaccga gagaatccct gcgccctaga cggccctgat
    19381 gagcatgctc gagattcagc gcgacgttcc gcttgctccg ctcacgacgt tcgggctcgg
    19441 agggcctgcc aaatacttcg cgcgcgtaga tgacgaggcg actctgctcg acgcgctccg
    19501 ctgggccgcc gacgaggggc tgcgatcggc gatcctgggt ggaggttcga atctcatcgt
    19561 gcccgacgag ggttatcctg gcctggtcgt tcagatggcg ctcggcgatc tcgagttcct
    19621 aacgggaggt tgcgtgatcg cgggcgctgg agtcccttgg aaggctgtcg tggacggcgc
    19681 gatctcgcgc gactgggcgg g
//
LOCUS       DASRZF010000268         2161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_545518, whole genome shotgun sequence.
ACCESSION   DASRZF010000268 DASRZF010000000
VERSION     DASRZF010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545518"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="VFG22_11895"
     CDS             complement(<1..784)
                     /locus_tag="VFG22_11895"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET6416991.1"
                     /translation="MSVLEVCLVRHAQSVSNAKGVWQGQGDSPLSEAGRRQVEALTGA
                     LAAERWDLILSSDLSRAADTAKASGASIEHDRQWREIHVGDWEGLSMEQVVERFPEQI
                     EALKARKTFAIGGGESWPEVFERADSSLAALRRRMPEGGRAIVFTHGGIIAALLSGLL
                     GARDRFPWPLGRMRNTGRTTIRFREDRVELVAHNDDSHLPSELRQSYQPRPDQVLVRM
                     TSKFGDRQARNTETADFNSAIKSARNKGAGGLVSVSGTSQEIA"
     gene            complement(781..1404)
                     /gene="clpP"
                     /locus_tag="VFG22_11900"
     CDS             complement(781..1404)
                     /gene="clpP"
                     /locus_tag="VFG22_11900"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HET6416992.1"
                     /translation="MSEDLATRAITIIEETHRGERAWDIYSRLLKDRIIFLGTPINDF
                     VANAVIAQLLYLESEDADKEITLYINSPGGLVSAGLAIYDTMSYVRCPVSTVCLGQAA
                     SMGAFLLAAGSKGLRRALPHSRVMIHQPLGGYQGQASDIEIHAREILNTKQRLNEILA
                     RHTGQDVETVRHDTERDKFLSALEAKEYGLVDDVIMPRKERGVAPAA"
     gene            1480..>2161
                     /locus_tag="VFG22_11905"
     CDS             1480..>2161
                     /locus_tag="VFG22_11905"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET6416993.1"
                     /translation="MLPAAIEMVRQLVATPSVSSQDLRFDQSNRGVIELLAEWSAGLG
                     LAVQIEALPGQTGKANLIAKLGEGEDGLVLSGHTDTVPYDADRWSDDPFALTERDGKL
                     HGLGTADMKGFFAAALHAIARFEPGALRRPITILGTADEESTMNGARALAKRGERLGR
                     YAIIGEPTGLAPIRKHKGVFYVQVTVSGKSGHASNPALGVNAIEGLQLVLAALQSWRD
                     TIGARYHDE"
BASE COUNT          364 a          675 c          701 g          421 t
ORIGIN      
        1 tggcgatttc ctgtgacgtg cctgataccg acaccagccc acctgcaccc ttattccgtg
       61 cagatttgat tgcagagttg aaatccgctg tttctgtatt tcgagcctgt ctgtcgccaa
      121 atttggacgt catacggacc agcacctgat cgggtctagg ttgatagctc tgacgaagct
      181 cggacggtaa gtggctgtca tcattgtggg cgacgagctc gacacggtcc tctcggaagc
      241 gaatcgtggt tctgccggtg ttccgcatcc tgccgagcgg ccaggggaac cggtcgcggg
      301 cgcccaacaa gcccgagagc agggcggcga tgataccccc atgcgtgaaa acgatggcgc
      361 gcccgccttc gggcatccgc cggcggagcg cagccaggga cgaatcggcc cgttcgaaga
      421 cttcgggcca gctttcgccg ccgccgatgg cgaaggtctt cctcgccttg agcgcttcga
      481 tctgctctgg gaagcgctcg acgacctgct ccatgctgag gccttcccag tcgcccacgt
      541 ggatctctcg ccactgccgg tcgtgctcga tgctcgcgcc cgatgccttg gccgtgtcgg
      601 cggcacggct gaggtccgag ctcaggatca gatcccagcg ctcagcagcc aatgctccgg
      661 tgagggcctc gacctgcctg cggccggcct cgctcagcgg ggagtctccc tgaccttgcc
      721 agacgccctt ggcgttggac accgactggg cgtgccgaac gaggcagact tcgagcacgc
      781 tcatgccgca ggagcgacgc cgcgttcctt acgcggcatg atcacgtcgt cgaccagccc
      841 gtattccttg gcctcgaggg ccgagaggaa cttgtcacgc tcggtgtcat ggcggaccgt
      901 ctcgacgtct tggcccgtat gccgggcgag gatctcgttg agccgttgct tggtgttcag
      961 gatctctctc gcgtggatct cgatgtccga ggcctggccc tgatagccac cgagcggctg
     1021 gtgaatcatg acgcggctgt gcgggagtgc gcgccgaagg cccttgctgc ccgctgccaa
     1081 gaggaaagcc cccatcgatg ctgcctgccc gagacaaacg gtcgagaccg ggcagcgcac
     1141 gtagctcatc gtgtcgtaaa tcgcgagacc ggcagacacg aggccgcccg ggctgttgat
     1201 gtaaagcgtg atctctttgt cggcgtcttc gctctctaga tagagcagct gggcgatcac
     1261 cgcgttggcg acgaagtcgt tgatcggcgt gccgaggaag atgatccggt ccttgaggag
     1321 ccggctgtag atgtcccagg cgcgctcgcc acggtgggtt tcttcgatga tggtgatggc
     1381 gcgtgtcgcc agatcttcgc tcatgtcgtg aacttagtct ctccgggcgc ggccgcaatc
     1441 cggattttgc tgcgccgggt tcagcgctat gttgtgaggg tgcttccagc cgcaatcgag
     1501 atggttcgcc agttggtcgc cactccgtcg gtcagctctc aggaccttcg tttcgatcaa
     1561 tcgaaccgtg gggtcatcga gctactggcc gagtggtcgg ctgggcttgg ccttgccgta
     1621 cagatcgagg cgctccccgg tcagaccgga aaggccaacc tgatcgcgaa gctcggcgag
     1681 ggcgaagacg gactggtgct ttcggggcac accgacacgg ttccctacga tgccgatcgc
     1741 tggagcgacg atccgttcgc cctcaccgag cgtgacggga agctgcatgg tcttggcaca
     1801 gcggacatga agggcttctt tgccgccgcc cttcatgcga tcgcccgctt cgaacccggg
     1861 gcgcttcgcc ggccgatcac catcctgggc acagccgatg aagagagcac catgaacggg
     1921 gcgcgtgcgc tcgcgaaacg gggagagcgc ctcggacgct atgcgatcat cggagagccg
     1981 accggactcg caccgattcg caagcacaag ggagtcttct acgttcaggt caccgtgtcg
     2041 ggaaagtccg gacacgcgag caaccccgcg ctaggggtga acgccatcga aggactgcag
     2101 ctcgttctcg ccgctttgca gagctggcgg gatacgattg gcgcgcgcta ccacgacgag
     2161 g
//
LOCUS       DASRZF010000269         2482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1579634, whole genome shotgun sequence.
ACCESSION   DASRZF010000269 DASRZF010000000
VERSION     DASRZF010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1579634"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="VFG22_11910"
     CDS             complement(<1..841)
                     /locus_tag="VFG22_11910"
                     /inference="COORDINATES: protein motif:HMM:NF017416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET6416994.1"
                     /translation="MMGRQRRDQGKLFYEFHLDDRIPQNHLLRRIDVFVTAALADLHQ
                     ELEPYYSDIGRPSVDPELMIRMLIVGYCCGIRSERRLTQEVELHLAYRWFCKLDLEDE
                     IPHHSTFSANRLGRFRESGLLRHVFERVVWAAMAMGLVKGEGFAVDASVLEANASRYH
                     GKAPAELDWSEKQRQTRAVAEYLAALDEAAEANPDRKVPKAISPSDPCSAWTAKANKR
                     VQFGYGLNYLIDIENTVIVDVEATPARTYDEVLATKTMIDRTERCFALRPKRLAADTA
                     YGTG"
     gene            complement(974..>2482)
                     /locus_tag="VFG22_11915"
     CDS             complement(974..>2482)
                     /locus_tag="VFG22_11915"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET6416995.1"
                     /translation="PATLPESTQTGSKLPNPSDAPFDTVVVLMMENRSFDHLLGWLPG
                     ANGRQQGLSYVDKDGASHETWPLAPDFQGCSYEDPDHTWPGIAIQYAGGRCDGFLQTA
                     KVGDTFPIGYYRDDDLPIISALAKDYTTFDNYFCSMMGPTWENRLFQLSGTTQLDDGW
                     CDFPRDKEEERRPVVIETTIFDRVRAAGLTAGYYYHESPVTGLFASRKYDDISYPIEQ
                     FWRDAREGKLANVVFVDPDYTDRAEDVGTSNDYHPWGNVLVAEGFLAQVHDALSKGPQ
                     WDRMVFVLNFDEHGGFFDHVTPPACKDDTKLAGDGPHPDLKRLGFRVPAIAMGPFAPR
                     KIEKAGPYEHCSILKMIEWRWGLEPMTMRDRYAKNLAEALDLTTRRDAIDLPVYDPPP
                     ARACAAGGGWLELPVSENGRVRVICDGLPDAECSATLTALENKLELAHVPVTKFHQAN
                     KVILELELSSSALALLKTSNRLPALLETRVVDAKGPVCRNVFSSMLIGPGAR"
BASE COUNT          467 a          772 c          756 g          487 t
ORIGIN      
        1 tgcccgtccc gtaggcggtg tcggcggcaa gccgcttggg cctcagcgcg aagcaccgct
       61 cggtccggtc gatcatggtc ttcgtcgcca gcacctcgtc gtaggttctg gccggcgtcg
      121 cctcgacatc gacgatgacg gtattctcga tgtcgatcag atagttgagc ccgtagccga
      181 actgcacccg cttattggcc ttggccgtcc acgccgaaca gggatcggac ggcgagatcg
      241 ccttcggcac cttgcggtca ggattcgcct cggcggcctc gtcgagggcg gcaaggtact
      301 cggcgacggc gcgggtctgc cgctgcttct cggaccagtc gagctccgcc ggtgccttgc
      361 cgtggtagcg gctggcattg gcttcgagca cgctagcgtc gaccgcaaat ccctcacctt
      421 tgaccagacc catggccatc gccgcccaaa caacccgctc gaagacgtgg cgcaacaagc
      481 cgctctcgcg gaagcggccc agccggttgg ccgagaacgt cgagtgatgg gggatctcat
      541 cctcgagatc gagcttgcag aaccagcgat acgcgaggtg cagctcgact tcttgggtga
      601 gcctgcgctc agagcggatg ccgcagcagt agcccacaat gagcatgcgg atcatgagct
      661 cgggatcgac cgaggggcgt ccgatgtcgc tgtagtaggg ctccagttcc tgatgaaggt
      721 cggccagggc agccgtcaca aacacatcga tccggcgcag aagatggttc tgcggaatgc
      781 gatcatcaag gtggaactcg tagaacagct tgccctggtc ccgcctctgc cgacccatca
      841 tcgcccggtc ctccgtcgcc gatctcgtca gcacacggaa tcaccgttcg gcgcctctct
      901 caacgcctca acgcccttta tcgacagcat cggtcagaag cggacgttcg tcgcgcctca
      961 gaccaatcag ttgttagcga gcgccgggcc caatcaacat tgatgaaaac acgttccggc
     1021 aaaccggtcc tttggcatcc acgacccggg tttccaataa ggctggcagg cggtttgaag
     1081 tcttgaggag cgcgagcgcg ctgcttgaaa gctccagctc caatatgacc ttattggcct
     1141 gatggaattt cgtgacgggc acatgcgcca attcgagctt gttctccagc gccgttaggg
     1201 ttgcgctgca ttcagcgtcg ggcagcccgt cgcaaatcac cctgacccgc ccgttctccg
     1261 aaaccggaag ctcgagccat ccaccaccag ccgcgcaagc cctcgccggc ggaggatcgt
     1321 agactggcag atcgatcgcg tcccggcgcg tcgtaagatc cagcgcctca gccaggttct
     1381 tcgcgtagcg gtcgcgcatc gtcatcggtt ccagccccca acgccactcg atcatcttga
     1441 ggatggagca gtgctcgtag gggcccgctt tctcgatctt gcgcggagcg aatggaccca
     1501 tggcgatggc cggaactctg aagcccagcc gctttaagtc gggatggggg ccatctccgg
     1561 caagcttggt gtcgtccttg cacgcgggcg gggtcacgtg atcgaaaaat cctccgtgct
     1621 cgtcgaaatt cagcacgaac accatgcgat cccattgtgg acccttgctc agtgcatcgt
     1681 ggacctgcgc caggaaacct tcggcgacaa gcacattgcc ccaagggtga taatcgttcg
     1741 acgtgccaac gtcctcggca cggtcggtgt agtcaggatc gacgaaaaca acattggcga
     1801 gcttgccttc gcgagcgtca cgccagaatt gctcgatcgg ataggagatg tcgtcgtatt
     1861 tgcggctcgc gaacaggccg gtgaccgggg actcgtggta gtaatatccg gccgtcaacc
     1921 ccgccgcccg cacgcggtcg aagatcgtgg tttcaataac cacgggtcgc cgctcttctt
     1981 ccttgtccct cggaaagtcg caccagccgt cgtcgagctg cgtcgtgccg gacagctgga
     2041 agagccggtt ctcccaggtc gggcccatca tcgagcagaa ataattgtcg aaggtcgtat
     2101 agtctttggc cagcgcgctg atgatgggca ggtcatcatc gcggtaatag ccgattggaa
     2161 atgtgtcacc gactttggcg gtctggagga aaccgtcgca acgtccgccg gcgtattgga
     2221 tggcgatacc gggccaagtg tggtccggat cctcgtatga acagccctga aaatcgggtg
     2281 cgagcggcca ggtctcgtga ctggccccat ccttatcgac ataggacaat ccctgctgcc
     2341 gcccgttcgc gcccggaagc cagcccagca ggtggtcgaa ggaacggttc tccatcatca
     2401 gcaccacgac ggtgtcgaag ggagcgtcgc tgggatttgg aagtttgctt cctgtttggg
     2461 tgctctccgg aagggttgcg gg
//
LOCUS       DASRZF010000270        17921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_546705, whole genome shotgun sequence.
ACCESSION   DASRZF010000270 DASRZF010000000
VERSION     DASRZF010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 17921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17921
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546705"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="VFG22_11920"
     CDS             complement(<1..743)
                     /locus_tag="VFG22_11920"
                     /inference="COORDINATES: protein motif:HMM:NF028121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HET6416996.1"
                     /translation="MGRLRGFGRAAFAAGLLLLSACDTEKLAADSTAELFGRAAPGIE
                     QHWDYVLVGKSFPASIIQLEGLLRVVPENEVIGLSLVAAYIGYGTGWIEDRVEVADIE
                     DDYEEATHLRRRALVMYNRAWDLSRHFLRLRDPGFDEALAGGVDTLETWVNERFQLKE
                     DAAILLWSGASLGARINMGMEDIDTVADLPLAKVLLERSVELDPDFMFMIGKMTMGVL
                     AASEFPPDMDRAKLILDEVLEKTERRNLMV"
     gene            848..1997
                     /gene="prfB"
                     /locus_tag="VFG22_11925"
     CDS             join(848..907,909..1997)
                     /gene="prfB"
                     /locus_tag="VFG22_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_146649532.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HET6416997.1"
                     /translation="MTAGECRETLQDLVQRMKALGGTFDLDGLRHNLDRLTNLSLAEG
                     FWDDQQRAQKMMRDRSSAEETIKEFERVESEARDLLELLDLAASEGDQGVIGDVAEQI
                     PVLEAEVRGLELKRMLTADDDTDAIVTINPGAGGTEAQDWADILLRMYLRWCERRGFK
                     TELVSKQPGDEAGIKDATFIARGTNAYGYLRAENGVHRLIRISPFDANKRRHTSFAGV
                     SVVPDLGDDLTEGEIEIRDEDLEVSTMRSGGAGGQHVNRTESAIRIKHIPTGFTVRCE
                     AERSQHQNRETAMKMLRGLLFEKARREREEAFEEAFLNDRADIAFGSQVRSYTLQPYT
                     MVKDERTDHKVNNAAAVLDGDLDEFIETYLLMNAEKKQKKKAGKAQGD"
     gene            2001..3608
                     /gene="lysS"
                     /locus_tag="VFG22_11930"
     CDS             2001..3608
                     /gene="lysS"
                     /locus_tag="VFG22_11930"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HET6416998.1"
                     /translation="MATEDELRQTRLSKAERLRELGVLPYPNTWRTNPSLEDQRRRLV
                     LLAANEEARSKLPLEEELGEDAPRVPLFGRVIAKRGPFLVIRTPHGDAQALVRPERLD
                     DREAELLKLLDLADHILVEGPAIRTKTGALAVSARRYEHLGKALLPPPAKWHGLKDVE
                     KRYRERYVDLFANPSVAEVFRARTVIIQSLRRFLDCLGFLEVETPLLHAIRGGATAKP
                     FDTHHNTLDLDLILRIAPELYLKRLLVGGFDRVYEIGRTFRNEGISTKHNPEFTILEF
                     YMAYANFEDLMDLSVDMLRAVDADVRARFAGLTSERSFDLQAEWKRVRMREAITERLE
                     RAGDGDVPTSPWSGVLAPDAVQDEQAFAAACQSMLPKLDAAAQKQLSRCESHGERVFA
                     VFELLVEPDLTTMYRTPSGAASVPVFITEFPWEVSPLARRNDQSPAFVDRFELFIEGR
                     ELSNAFSELNDPDDQAERFESQLRNRERGDDEAMDFDADYIRALSHGMPPAAGFGLGV
                     DRLVMLLCNQPSIRDVLLFPLLRPEAE"
     gene            3605..5656
                     /locus_tag="VFG22_11935"
     CDS             3605..5656
                     /locus_tag="VFG22_11935"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6416999.1"
                     /translation="MTEARPLGFWTAVLCGVGIAQIAAVLVFGATSTDWFQATVLDEL
                     LWVRLSIAWTTTQLSLALLSLAVLQRGIRRPRGSRGRARTVALALTAGIHVLMSSMSV
                     GILLWASRVSVGHVLGGTIGSLLFGLTHLAYPDLDLDWVPTLYQTASTLQSLSAALLV
                     VLSITCWFLVPVERRRRFWWGFDATLVLAFLGVSLRFPFSPIDGDDLSLLHAVARVGV
                     TTLFAVRLTLRLVGPLLSAFERIGFRSMVAARHLRAKKTGFLAASSTLSILAVAVSSC
                     MLISVLSVMGGFRADLKKKILGNHAHIVIDKAHDEISDWQPLLRRTRETEGVVGATPY
                     LQVEVMVSSLSNRAGAILRGIDPHTVSEVTDLGRNMTHGKLEFLEHPEQILDSSIDTT
                     KAGPMDAMIEEAERTAARGLPDENEEDAMPGLIVGQELARALRLVVGDRVTVVSPRGD
                     LGPTGPIPRTRAFRIAGIFYSGMYEYDMKHAYIDLGVAERFLRSEGRISGIEIKVDEL
                     ERAPVLAAELRSTIGAQGLRVRDWKMMNQQLFGALALEKLAMFITLGIAILIAGFSVF
                     GTLTLLVQEKRREVGILKAMGTSAPSVIRIFVIEGLLIGTAGALMGLGLGFVLTFAAE
                     HFGIRMNPEVYYIDKLPVHLDPTEFGMVGAATVVVCLAATLFPAYQASRVRPVDALRY
                     D"
     gene            5672..6388
                     /locus_tag="VFG22_11940"
     CDS             5672..6388
                     /locus_tag="VFG22_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6417000.1"
                     /translation="MSQPLVTVKNVTKTFEHEGRSLQVLKGIDLEIGSGEMVTIVGPS
                     GAGKSTLLHLIGTLDLPSEGQILYEGRDVTRLGSSDLAEFRNRSIGFVFQFHHLLPEF
                     TALENVMMPGLIQGRHKYETRARQLLEEVGLTERLTHRPGELSGGEQQRVALARALLM
                     EPKLVLADEPTGNLDSQTSASVHALFFDLNRRHGITFLIVTHSRDFAAMMPRQVSMKD
                     GRIDSDERRAQPLENQGKPA"
     gene            6514..9033
                     /gene="bamA"
                     /locus_tag="VFG22_11945"
     CDS             6514..9033
                     /gene="bamA"
                     /locus_tag="VFG22_11945"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HET6417001.1"
                     /translation="MPNPPGGETRPVRTSPAPQRPPDPLTGDGSLGGDGDPYEPVEDA
                     EAGIEVPRTSCHGKEIRRIVVIGARRVDPEDVRATMKLRRGSTCTDPAVTRDAKALWN
                     MAYFDDLQIESDPIGAGIKLTIRLVERPAIRKVVYEGNDEVDKDDIDETISLEPGTIL
                     SVPDVEAQADAMRQLYSEEGFFLADVKYELRRVPGDSNQVDIVFKIDEGAEVEVRRIS
                     FVGNEQLPSAELLKIMQTNETGMFSFLSDRNKFSRPAFDEDLTRVQAWYYDKGFLMMR
                     PGRPSVELTADRAYVDITIPVEEGPRFRIREFDVMETDGAGNEVETLAPKAQLREMVR
                     LKSGDWFSRSKIAEGIEGISRTYRDSGYALVEMVPGTDLDETNHLVDVTINILRGPLV
                     YIERLHVRGNTKTRDAVIRREFRISEGDLYSQSKLEDGRALVTQLGYFERVDVSEEEG
                     TARDQMVINVEVAEKPTGTFQVGAGFSSLESFIVTAQVQQQNLFGRGQSLGLNLQVSG
                     IRQQIDLNFVEPWFLGTEWSLGFGVFKRIRDFRDFRQDSTGANVTTGHPIWNPRFRIF
                     LRYEFENVNISQPRGNLLGTGGQLANTFSNIFIANAFRDGITSSLRLTLNWDSRDNRQ
                     TTARGIFANYAVQVADQYLGSDNTFVRQTAFARFYQRAFGPIVFKVNMEMGLLTSRKI
                     VPVFERFYLGGIYTIRGYRLQSVGPQAGLAAAGDPNLPIIAQGRPIGGDFQAFYNVEF
                     EFPIVEAVGIKGVIFTDGGNAWNLFAFRDQFGNCQAPQAPQIEASSRPCGINGFLRYS
                     VGFGLRWFSPLGPLRFEWGIPIRRRPQDQPIRFEFTIGQSF"
     gene            9127..9678
                     /locus_tag="VFG22_11950"
     CDS             9127..9678
                     /locus_tag="VFG22_11950"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HET6417002.1"
                     /translation="MRFCRIVTIALLAVILVGSASTVDAQAKIGVVDLQRAINETEDG
                     RQAKRRLKKVFDERQQILNEKQESLKAQKESLERQQEVLSQDALKKKVEKYQADLMEL
                     QNEYVQYQEELQTKEAELTQKILEKMQGILRRIGQTEDYTLIIEANEGGVVWVPSNLD
                     LTDVLIQRYNKQAKEGQSPKKPK"
     gene            9724..11145
                     /locus_tag="VFG22_11955"
     CDS             9724..11145
                     /locus_tag="VFG22_11955"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET6417003.1"
                     /translation="MVRFWALAVIALTTLGVAAEVIAEEAGAGEAEPGDEEPARSRVY
                     QIDEYTQAAILRYPGLKAAAADIDAAEARLSEAKLSPFMQFQGQATLFVRPGAQGTPT
                     FSPDSQIPWGNRWGAGGEVGIEGGIPIYTFGKYRAGKKAAAAGISASEHERDETITRL
                     VFDVRRAYYGTELALDLQAMISEGKDKLRTAVDKLDARLQADDPRAQQTDYWRLVAAL
                     SDLESRESEALRLEASARAALEILSGIEPAIVPECPLEAVKSEVVELGEHVDRALANR
                     PEIFQLEAAQSARDANLTVKRAGYLPDILLALRATFARTPGVTDINNPFIIDRGNFAG
                     AFAGILAQWKLDFGGTHARVKAAKAEIQSLKAKTEEAELGIELEVTDLYERLQDAKRR
                     LASWKRSEKETRKWFVSAGQGYEVGTTSAKDLVDAIQAYFNARARRLSATAEYNLAIA
                     GLERATNMPLVSPKGWRPVDCEE"
     gene            11177..11827
                     /locus_tag="VFG22_11960"
     CDS             11177..11827
                     /locus_tag="VFG22_11960"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6417004.1"
                     /translation="MARLSILSGLLVVSLLVSARAFAEEPPRAMADLGVSAAQADGAQ
                     QFIRTKHNKVRAVLRKPDTPKRADELTELLGEFLDYDRLAKLSLDKEWGKRTPKERDH
                     FVSLLRQLVERQYQRNMESTLQYKVSWVGTEPIETGVKVKSSAKSVKKKRQPPITIDY
                     SMSPAGEEWKVFDIFTDDVSLVKNYKRQFRRVIADEGWSGLIDRMEKKLNAEDDLI"
     gene            complement(12577..13305)
                     /locus_tag="VFG22_11965"
     CDS             complement(12577..13305)
                     /locus_tag="VFG22_11965"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET6417005.1"
                     /translation="MDSNAPAMQNGSGARRMRTTEERRSRIMARCRIFEALTSELRER
                     ILSGSALVDADRRESLCMPSDPAIFVIGSGRVRRVRRTPERELTLGYYGPGDLVGEMS
                     LVDPEARLETVVLDYVEALRIPPRLIKMVMSEDLEFASAMHRLMGERRLLAERRIDAL
                     LTRSVESRVAEFLLDASSRHGIPDSRGILIGVKFTHQEIASYVGSTRETVTLVLGAFK
                     RAGLIATDHRRVVIKDIAGLRKRV"
     gene            13477..13989
                     /locus_tag="VFG22_11970"
     CDS             13477..13989
                     /locus_tag="VFG22_11970"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HET6417006.1"
                     /translation="MRLYSGKIGPIVDDLVRELTAQEDIEVEDVAEVRLDLEAVLKEY
                     VRRERQMVDEAKDRMEREGLGYSKLGRMRQQLAKEMGFPQQDEVLPYLVDQLINMLFH
                     SSNVVEIYADDVTIRKKMTPILRRHMEVETGLDAEVRSKIKNLQEGTSAFEVEYAKMM
                     DQIKHKRGLE"
     gene            14006..14872
                     /locus_tag="VFG22_11975"
     CDS             14006..14872
                     /locus_tag="VFG22_11975"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HET6417007.1"
                     /translation="MTGYGSGRAALGDGEVVLDIRTVNHRFLDVRVRLPSRIQTRTPI
                     VERVIRRRLERGRVDVTARFEGQTLPQPRLDLERARAVYSELTALRDELNPGEPVPLS
                     VLSSVPDLFIADRSIDDEALDRALSVAAEAACSAVLAMREKEGNALASELIDRLSELL
                     ANLGTLSEAVPSMLEDRRLRLRDRLDALLVDTQAALDPSRLEQEIALMADRSDVAEEL
                     VRLESHRVQMLELIENSDGAVGKRIDFLLQEMAREANTIGSKVQDGATTAHVVGLKAC
                     IEKMREQAQNVL"
     gene            14918..15547
                     /gene="gmk"
                     /locus_tag="VFG22_11980"
     CDS             14918..15547
                     /gene="gmk"
                     /locus_tag="VFG22_11980"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HET6417008.1"
                     /translation="MSVAAPTDSDLILLIISSPSGAGKTTLTRRLLQEFSDFRFSVSH
                     TTRLPRTNEVDGQDYHFVSESEFRNVIASDGFAEWAEVHANLYGTSVAEIELAREAGK
                     RGVLFDVDYQGARQIKKRFPHAVGVFILPPSMTELRRRLDGRGSDDAESRKRRFDKAR
                     DEIGHYPFFDYMIVNDELQRALAELRGIVLAEGCRQWRVAAKAEALLQE"
     gene            complement(15550..17718)
                     /locus_tag="VFG22_11985"
     CDS             complement(15550..17718)
                     /locus_tag="VFG22_11985"
                     /inference="COORDINATES: protein motif:HMM:TIGR04551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04551 family protein"
                     /protein_id="HET6417009.1"
                     /translation="MSDIEVTAAWLKAGVAPVKAAIASLTRLIPACYARDRTGSMRRF
                     FAIICLVFLGATSAAAQRSSTGDVSDPGVQGTSETEAQEGISEVEDAEVEPSREEDLP
                     PQLRAPSIEGLQMPSAPPSDEESGDEFDEADFDEDKMASDDAALLGRAEPPAADPTLA
                     TWTNPRPIFSLNGYLRVRGNYVNNYSLRRQPLARFPDDPFVRWIPVDNSPSNGNNGVL
                     PQGGCTGPPTSVAGPAERCDGTATLRYANMRLRLQPTLAPSDNIRVHMMIDVFDNLVL
                     GSTPEVKSVNQFGQVVGRAPGVPVDSLTLTSPPPESYRNTVGDNIIVRRAWGEVTNST
                     IGQLRFGRMGNQWGLGMLWNAGTGTNNLDTELDSDFQSEVDRIQLIGKFKGMFFGVSW
                     DFANKGYIYDPVENIQGIPYDASRLDDTRQWSFLFARRMDALAQEKRIASGKWVINGG
                     AYFIYRSQFLSTSTAPLIGTISDVENSFVRRDAKVYIPDGWVQVLRKNLRLEIEFAGI
                     FGQIQNITAGGFPPSAEDEPYKLRQWGFAFEGEYRFLQQKLGVILKGGTASGDPDVVG
                     LSQYENLAAQPVGKKNVTNFSFHPDYRIDLIFWRSIMGRVAGAYYLAPAVSYDIIRTD
                     FGRVLGARLDFIYSRAMYAQQTYSSEPNLGAELDISIYYRTENGPSFKDGFFAAAQFG
                     IFFPLNGLQYLQVDGISEPGTEGLGISRAMVLRLILGIAY"
     gene            17708..>17921
                     /locus_tag="VFG22_11990"
     CDS             17708..>17921
                     /locus_tag="VFG22_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HET6417010.1"
                     /translation="MSDTVRFVALGGLNEVGMNCAALTCGDTTIAIDCGIGFTDEHGA
                     ELVHPNFEYLFQERERLRAIVITHGHE"
BASE COUNT         3571 a         5639 c         5485 g         3226 t
ORIGIN      
        1 accatcaagt tgcgtcgctc ggtcttctcg aggacctcgt cgaggatcag cttggcgcgg
       61 tccatatcgg gcgggaactc cgaggccgcc agcacgccca tggtcatctt cccgatcatg
      121 aacatgaagt ccggatcgag ctcgaccgag cgctctagga gcaccttggc gagcggaagg
      181 tccgcgacgg tgtcgatgtc ctccatgccc atgttgatgc gagcgccgag cgaggcaccg
      241 gaccagagca agatagcggc gtcttctttg agctggaacc tctcgttgac ccaggtctcg
      301 agggtgtcga cgcctccggc aagcgcttca tcgaagcccg gatctcgaag ccgaaggaag
      361 tgtcgcgata gatcccaggc tcggttgtac atgaccaatg cgcgccgccg aagatgggtg
      421 gcttcttcgt aatcgtcctc gatatccgca acctcgaccc tatcctcgat ccatccggtg
      481 ccgtaaccga tgtacgcggc caccagcgaa agcccgatca cctcgttctc gggaaccact
      541 cgcagaaggc cttccaactg aatgatgctc gcgggaaagg acttcccgac cagcacgtag
      601 tcccagtgct gctcgattcc gggcgctgcc cgcccgaaga gctcggcggt cgaatccgcg
      661 gcgagcttct cggtatcaca cgcgctcagc aacaggagcc ccgccgcaaa cgcagcgcgc
      721 ccgaaacctc tcaatcggcc catggcgcga aagtgaaccg gaatctccct tccaacacaa
      781 gtgaaaacaa gatgcggtgc cgagtttgac agttggtagg tggcggggta ggtccgcgcg
      841 cagagtcatg accgcagggg aatgtcgcga aacacttcaa gacctcgtac agcgcatgaa
      901 agcgctgggg aggtaccttt gacctcgacg gacttcgcca caatctcgac cggctcacca
      961 atctgagcct cgcggagggc ttctgggacg accagcagcg ggctcagaag atgatgcgtg
     1021 atcgctcctc cgcggaggaa accatcaagg aattcgagcg ggtagagagc gaagcgagag
     1081 atcttctcga gctcctagac ctcgcggcga gcgagggcga tcagggcgtg atcggcgacg
     1141 tagccgagca gatacccgtc ctcgaagccg aggtacgtgg cctcgagctc aagcgcatgc
     1201 tcaccgcgga cgacgacacg gatgcgatcg tcaccatcaa tccaggcgcc ggtggcaccg
     1261 aggcccagga ctgggcggat attctgctgc gcatgtatct gcgatggtgc gagcgccgcg
     1321 gtttcaagac cgagttggtg agcaagcagc cgggcgacga agcgggcatc aaggacgcca
     1381 cgttcatcgc gcggggcacg aacgcttacg gatacctccg agcggagaat ggggtccacc
     1441 gtctcattcg aatcagccct ttcgacgcca acaagcgccg acacacctcg tttgccggcg
     1501 tgagcgtcgt gcccgacctc ggcgacgacc tcacggaagg cgagatcgaa atccgtgacg
     1561 aagatctcga ggtgagcacc atgcgctcgg gaggcgcggg cggccagcac gtcaaccgga
     1621 cggaatctgc cattcgcatc aagcacatcc ccacgggctt cacggtccgc tgcgaggccg
     1681 agcgttctca gcaccagaat cgagaaaccg cgatgaagat gctccgtggc ttgctcttcg
     1741 agaaagcccg gcgcgagcgc gaagaggcat ttgaagaagc cttcctcaac gaccgcgccg
     1801 acatcgcctt cggctcgcag gtccgtagct acactttgca gccctacacg atggtcaagg
     1861 acgagcgcac ggaccacaag gtcaacaacg ccgcggccgt cttagacggg gacctcgacg
     1921 aattcatcga aacctacctc ttgatgaacg ccgagaaaaa acagaagaag aaggcgggca
     1981 aagcccaagg ggactaacaa gtggcgaccg aagacgagct gcgacagacc aggttgagca
     2041 aggccgagcg actacgcgag ctcggcgtcc tgccctaccc gaacacctgg cgcacgaacc
     2101 cctcgctcga agatcagcgc cgtcgcctcg ttcttctcgc cgcaaacgaa gaggcccggt
     2161 ccaagctgcc tttggaagaa gagctcggcg aagacgcgcc gcgggtgccg ctcttcggcc
     2221 gcgtcatcgc aaagcgcggg ccttttctgg tcatccgcac accgcacggc gacgcgcagg
     2281 ccctcgtgcg tcccgaacgc ctcgacgata gggaagcaga gctgctcaag ctcctcgact
     2341 tggcggatca catcctggtg gaaggcccgg cgatccgcac caagaccggg gctcttgcgg
     2401 tgagcgcgag acgatacgag catcttggaa aggccctgct gccccctccc gcgaagtggc
     2461 acggcctcaa agacgtcgag aagcgctatc gagagcgcta cgtggacctc tttgccaacc
     2521 catccgtcgc cgaggttttt cgcgcgcgca ccgtgatcat ccagtcgctg cgccgtttcc
     2581 tcgactgcct ggggttcctc gaagtggaaa ccccactgct ccacgcgatc cgggggggcg
     2641 ccacggcgaa gcccttcgac acgcaccaca atacactcga tctcgacctg atcctccgca
     2701 tcgcccccga gctctatctc aagcgactgc tcgtcggagg cttcgatcgc gtctacgaga
     2761 tcggacgaac gttccggaac gagggcatca gcaccaagca caaccccgag ttcacgatcc
     2821 tcgagttcta catggcctac gccaacttcg aggacctcat ggatctctcg gtcgacatgc
     2881 tccgcgcggt cgacgccgat gtgcgcgcgc gtttcgcggg cctcacctcg gagcgcagct
     2941 tcgacctgca agcggagtgg aagcgggtac gcatgcgaga ggcgatcacc gagcgcctgg
     3001 aacgcgccgg ggatggcgac gttccgacga gcccgtggag cggcgttctg gcaccggacg
     3061 cggtccaaga cgagcaagcc tttgccgcgg cttgccagtc gatgctgccc aagctcgatg
     3121 ccgctgcgca aaagcaactc tcccgctgcg agtcccacgg cgagcgcgtc ttcgcggtct
     3181 tcgagctctt ggtcgagccc gacctcacca cgatgtaccg gaccccgagc ggcgctgcgt
     3241 cagtgcccgt cttcatcacc gagttccctt gggaagtgtc gcccctcgcg cgccgaaacg
     3301 atcagagtcc ggccttcgtc gaccgcttcg agctcttcat cgagggccgt gagctcagca
     3361 acgcattcag cgagctcaac gacccggacg atcaggccga gcgcttcgaa tcacagttgc
     3421 gcaatcgcga gcgcggcgac gacgaagcca tggacttcga cgccgactac atccgggcgc
     3481 tgtcacacgg catgccccct gccgcgggct tcggcctagg cgtggaccgg ctcgtcatgc
     3541 tcctctgcaa ccagccatcg attcgagacg tgctgctgtt ccccttgctg cgcccggagg
     3601 ccgagtgacc gaagcccggc cgctcggttt ctggacggcg gttctctgtg gtgtgggtat
     3661 tgcgcaaatc gccgccgtgc tcgtcttcgg ggcgacgagc accgactggt ttcaggcgac
     3721 ggtgctggac gagctgctct gggtacggct cagcatcgcc tggaccacga cccagctcag
     3781 tcttgcgctg ctgagcctcg cggtccttca gcgcggcatc cgacgcccac gtggtagccg
     3841 agggcgtgcc cgcacggtgg ccttggcact caccgccgga atccacgtgc tcatgagcag
     3901 catgtccgta ggcattctct tgtgggccag ccgtgtctcg gtcggacacg tgctcggcgg
     3961 aacgatcgga agccttctct ttggcctcac gcacctggcc taccccgatc tcgacctcga
     4021 ctgggtaccc acgctctatc aaacggccag cacgctccag agcttgagcg ccgcgctgct
     4081 cgtcgtcctc agcatcacct gctggttcct ggtgcccgta gaacgccgga gacgcttctg
     4141 gtggggcttc gatgccacgc tcgttctcgc tttcctaggg gtctctctcc ggttcccatt
     4201 ctcgcccatc gatggcgacg acctgagcct tcttcacgcg gtcgcccgag tcggcgtcac
     4261 gaccctcttc gctgtgcgac tcacgctccg cctggtcggc ccgcttctct cggccttcga
     4321 gcggatcggt ttccgctcga tggtcgctgc ccgacatctc cgcgcaaaga agaccggctt
     4381 cctcgcggcg agcagcacgc tgtcgatcct cgcggtcgcg gtctcgtcct gcatgctcat
     4441 ctcggttctt agcgtgatgg gcggttttcg ggccgacctc aagaagaaga tccttggcaa
     4501 tcacgcccac atcgtgatcg acaaggcgca cgacgagatc tcagactggc agccgcttct
     4561 ccgccgcacg cgcgagaccg aaggcgtcgt cggcgccacc ccctaccttc aggtcgaggt
     4621 catggtttcg agcctttcaa accgcgccgg cgcgatcctt cgtggcatcg atccacatac
     4681 cgtctcggag gtgaccgacc tcggccgaaa catgacccac gggaagctcg aattcctaga
     4741 gcacccggag caaatcctcg actcctcgat cgatacgacc aaggcgggcc cgatggacgc
     4801 gatgatcgaa gaagcggagc ggacggcggc ccgagggctt cctgacgaga atgaagagga
     4861 cgccatgcca ggcctcatcg tcggtcagga gctcgcgcgg gcgctgcgat tggtggtcgg
     4921 cgatcgcgtc acggtggttt ccccgcgcgg agacctaggc ccaaccggcc cgattccgcg
     4981 cacccgagcc tttcgaatcg ctggaatctt ctacagcggc atgtacgagt acgacatgaa
     5041 gcacgcctac atcgatctcg gcgtggccga gcgctttctg cgatcggagg gtcgaatcag
     5101 cggtatcgag atcaaggtcg acgagctcga acgtgccccg gtgctcgcag cagagcttag
     5161 gtcgacgatc ggggcacaag gcctccgagt gcgggattgg aagatgatga atcagcagct
     5221 cttcggcgcg ctcgcactcg aaaagctcgc catgttcatc acgctgggga tcgcgatcct
     5281 catcgccggc ttttcggtat tcgggacgct caccctcctc gtccaggaaa aacgccggga
     5341 ggttggcatt ctcaaggcca tgggcacctc ggcaccgagc gtgattcgga tcttcgtgat
     5401 cgaaggcctt ttgatcggaa ccgcaggggc gctcatgggg ctcggacttg ggttcgttct
     5461 cacgtttgcc gccgaacact tcggaatccg aatgaatccc gaggtctact acatcgacaa
     5521 gctccccgtg catctcgatc cgacggagtt cggtatggtc ggcgcagcaa cggtggtggt
     5581 atgcctggcg gcaaccctct ttcccgcgta tcaagccagt cgggtgcgac cggtggacgc
     5641 cctccgttac gactgacttc tccgattgtt gatgtcccaa ccactcgtca ccgtaaagaa
     5701 cgtgaccaag accttcgagc acgaaggccg gtccctccag gtcctcaagg gcatcgacct
     5761 cgagatcggc agcggcgaga tggtgacgat cgtgggtcca tcgggtgcag ggaagagcac
     5821 gctgctccat ttgatcggga ccctcgacct gccgagcgag ggtcagatcc tgtacgaggg
     5881 ccgggacgtg acgcgtttag gaagctcgga tctcgctgag tttcgcaacc gaagcatcgg
     5941 tttcgtattc caatttcacc atctcctgcc cgagttcact gctctggaaa acgtgatgat
     6001 gcccggcctc atccaaggcc ggcacaagta cgagaccagg gcacggcaac tgctcgaaga
     6061 ggtgggcctc accgagcggc tcacccaccg ccccggtgag ctgtccggcg gtgagcaaca
     6121 gcgtgtggcc ctcgcacgtg cacttttgat ggagcccaag ctcgttctcg ccgatgagcc
     6181 gaccgggaac ctcgacagcc aaaccagcgc ctccgtgcac gccttgtttt tcgatttgaa
     6241 ccgccgacac ggaatcacct tcttgatcgt gacccacagc cgtgatttcg cagcgatgat
     6301 gccgaggcag gtctcgatga aggatgggcg tatcgattcg gacgaacgcc gcgctcaacc
     6361 ccttgaaaac caaggaaaac cagcttgacc ccttaacagg gcacggctat agccgctcgg
     6421 atcgctaccc ccacaatgcg cccttccgcc cctgccatag ccctgatgct cgcagggctc
     6481 gcttccgtgg cgggcgctca gagtgaggag aacatgccga accctcccgg aggggagacg
     6541 cgtccggtgc ggacatctcc ggcaccgcag cgcccgcccg atccgctcac cggcgacggg
     6601 agcctcgggg gcgacggcga tccctacgaa cccgtcgagg acgcggaagc ggggatcgag
     6661 gtgccgcgaa ccagctgtca cggcaaggag attcgccgaa tcgtggtcat cggtgcgcgg
     6721 cgcgtcgatc cggaggacgt tcgggccacg atgaagctcc gccgaggctc gacgtgcacg
     6781 gatccagcgg taacccggga tgcgaaggcg ctttggaaca tggcgtactt cgatgacctc
     6841 cagatcgagt ccgatcccat cggcgctggg atcaagctca cgatacggct cgtcgagcgg
     6901 ccggcgattc gcaaggtcgt ctacgagggt aacgacgaag tcgacaaaga cgacatcgac
     6961 gagacgatca gcctcgagcc ggggaccatc ctctcggtgc ccgatgtgga ggcgcaggcc
     7021 gacgcgatga ggcagctcta ttcggaggaa gggttctttc tcgcggacgt gaagtacgag
     7081 ctgaggcggg tccctggcga tagcaaccag gtcgacatcg ttttcaagat cgacgaaggc
     7141 gctgaagtag aagtgcggcg catcagcttc gtcggcaacg agcagctccc gtccgccgag
     7201 cttctgaaga tcatgcagac caacgagacc ggcatgttct cgtttctcag cgaccgcaac
     7261 aaattcagtc gcccagcctt cgacgaagac ctcacgcgag tccaggcctg gtactacgac
     7321 aagggcttcc tgatgatgcg cccgggccgg ccttcggtag agctcaccgc cgaccgcgct
     7381 tacgtcgaca tcaccatccc ggtcgaagaa ggcccgcgtt ttcgtattcg cgagttcgat
     7441 gtgatggaga ctgacggtgc cggcaacgag gtagagaccc tcgcgcccaa agcgcagctt
     7501 cgcgaaatgg tgcggctcaa gtcgggtgac tggttcagcc gcagcaagat tgcggaaggc
     7561 atcgagggca tcagccgaac ctaccgggac tcggggtacg ccctggtcga gatggtcccc
     7621 ggcaccgacc tcgatgagac caatcacctg gtcgatgtga cgatcaacat ccttcggggc
     7681 ccgctggttt acatcgagcg ccttcacgtg cgcggtaaca ccaagactcg cgatgccgtc
     7741 attcgccgcg agtttcgaat cagcgaaggc gacctctaca gccagagcaa gctcgaagac
     7801 ggccgcgcgc ttgtcactca gctcggatac ttcgaacggg tcgacgtatc cgaggaggag
     7861 ggcactgcgc gcgaccagat ggtgatcaac gtcgaagtcg ccgagaagcc aaccggaaca
     7921 ttccaggtcg gagcgggctt ctcgtcgctc gagagtttca tcgtcactgc gcaggtccag
     7981 caacagaact tgttcggccg ggggcaatct ctcggattga acctgcaggt gtccggcatc
     8041 aggcagcaaa tcgacctcaa cttcgtcgag ccctggttcc tcgggacgga gtggagtctc
     8101 ggcttcgggg tcttcaaacg gatccgcgac ttccgcgatt tccgccagga ctccacgggt
     8161 gccaacgtca cgaccgggca ccctatctgg aatccgcgtt ttcgcatctt ccttcggtac
     8221 gagttcgaga acgtgaatat ctctcaacca cggggcaacc tgctcggcac gggcggccag
     8281 ttggccaaca cattttcgaa catcttcatc gccaacgcgt tccgcgacgg catcacgagc
     8341 tcgctcaggc tcacgctaaa ctgggacagc cgcgacaatc gccagaccac cgcgcgcgga
     8401 atcttcgcga actacgccgt gcaagtcgcc gaccagtacc tcggctcgga caacaccttc
     8461 gttcgccaga ccgcattcgc gcgcttctat cagcgggcgt tcggtccgat cgtcttcaag
     8521 gtgaacatgg aaatggggct gctaacctct cgaaagatcg tgcccgtgtt cgagcgcttc
     8581 tacctcggtg gcatctacac gattcgcggt tatcgattgc aatcggtggg accacaggca
     8641 ggtcttgccg ccgcaggcga tcccaatttg cccatcatcg cgcagggtcg cccgatcggc
     8701 ggcgacttcc aggcgttcta caacgtcgag ttcgagttcc ccatcgtcga agccgtgggc
     8761 atcaagggcg tgattttcac cgatggcggc aacgcctgga acctctttgc cttccgcgat
     8821 cagtttggaa actgccaagc cccgcaggcc ccgcagatcg aggcctcgtc gaggccctgt
     8881 gggatcaacg gtttcctccg ctattccgtc ggctttggtc tgcggtggtt ctcgccgctc
     8941 ggcccgctcc gattcgaatg gggcattccc atccgccgtc gcccgcagga ccagccgatt
     9001 cgcttcgaat tcaccatcgg tcagtccttc tgaacgaatg aaagccgctg gctgagcgta
     9061 tcacccctgc ggcttgacct gccgacgcca aacggctagc taggccgggc ctgaaaggat
     9121 gctctgatgc gcttttgcag aatcgttact atcgccctcc ttgctgtaat tctcgttgga
     9181 tcggcgtcaa ccgtagacgc tcaggctaag atcggcgtcg tcgatcttca aagggcgatc
     9241 aacgaaaccg aagacggccg gcaagccaag cgacggttga agaaagtctt cgacgagcgg
     9301 cagcagattc tcaacgagaa gcaggaaagt ctgaaggccc agaaagaaag cctcgagcgg
     9361 cagcaggagg tgctgagcca ggatgcgctc aagaagaagg tcgaaaagta ccaggcagac
     9421 ctgatggagc ttcagaacga gtacgtgcag taccaagagg agcttcagac caaggaagcc
     9481 gagctcactc agaagatcct cgaaaagatg cagggtattc tccgccgcat cgggcaaacc
     9541 gaagactaca cgttgatcat cgaggcaaac gaaggcggag tcgtctgggt tccttcaaac
     9601 ctcgatctca cggacgttct catccagcgc tacaacaagc aggccaaaga gggccagagc
     9661 cccaagaagc ccaagtaagc gcgcattcgt ggccgaacgg gccgtttcca gctacaaaag
     9721 cgcatggtca ggttctgggc gctagcggtc atcgcgctga cgacgctagg tgtcgccgct
     9781 gaggtgatcg ccgaggaggc aggcgccggc gaggccgaac ccggcgacga ggagcccgcg
     9841 cgcagccgcg tgtaccaaat cgacgagtac acgcaggccg ccatccttcg gtatccgggc
     9901 ctcaaagccg cagccgcgga catcgacgcc gcagaagcgc gacttagcga ggcgaagctc
     9961 tcgccgttca tgcagtttca aggccaggcc acgttgtttg tgagacctgg cgctcaggga
    10021 acgccgacat tcagcccgga ttcccagatt ccctggggca accgatgggg tgcgggcggc
    10081 gaggttggca tcgaaggcgg catcccgatc tataccttcg ggaaataccg ggccggcaag
    10141 aaggccgcgg cggccggaat ctccgcgtcc gagcacgaac gggatgaaac gatcacacgt
    10201 ctggtgttcg acgtccgtcg ggcctactac ggaaccgagc tcgcactcga ccttcaggcg
    10261 atgatctcgg agggcaaaga caagctccgg acggccgtgg acaagctgga cgcacgcctt
    10321 caggccgacg atccgcgggc acagcaaacc gactattggc gtctcgtggc ggcgctgtcc
    10381 gacctggagt cgcgcgagtc ggaagccctg cgattggagg cctcggcccg tgcggcgctc
    10441 gagatcctct cgggcatcga gcccgccatc gttcccgagt gtccgctcga agccgtgaag
    10501 agcgaagtcg tcgagctcgg cgagcacgtt gaccgtgccc ttgcgaatcg cccggagatt
    10561 tttcaactcg aagcggcgca atcggcgagg gatgccaacc tcacggtgaa gcgcgccggc
    10621 tacctgcccg acattctgct cgccctgcgc gcaaccttcg cgcggacgcc gggcgtcacc
    10681 gacatcaaca acccgttcat catcgaccgt ggcaacttcg caggagcgtt tgcgggtatt
    10741 ctggcgcagt ggaagctcga cttcgggggg acccacgcac gtgtcaaagc ggccaaggcc
    10801 gagatccagt cactcaaggc gaagaccgag gaagccgagc tcggcatcga gctcgaggtc
    10861 acagacctct acgaacggct ccaggacgcc aagcggcgac ttgccagttg gaagcggtcc
    10921 gagaaagaaa cacgcaaatg gttcgtgtcg gctggacagg gctacgaagt cggcaccacc
    10981 tcggccaagg acctggtcga tgcgattcaa gcgtacttca acgcgcgcgc gaggcggctc
    11041 tcggccacgg ccgaatataa tctcgcgatt gcgggactgg aaagagcgac caatatgcct
    11101 ctcgtctccc caaagggttg gcgccccgtc gactgcgagg aataaagcgt cgattggcct
    11161 cgtctacaag gaaaccatgg cacgtctatc aatactctca ggtctcttgg tcgtttcatt
    11221 gctcgtctcc gcccgagcat tcgcggagga accgccgcgc gcgatggcgg atctcggggt
    11281 gagcgccgct caggccgacg gcgcacagca gttcattcgc accaagcaca acaaggtgcg
    11341 ggctgtcctg cgcaagcccg ataccccgaa gcgcgcagat gagctcaccg agctgctggg
    11401 cgagttcctc gactacgacc gccttgcgaa gctctctctc gacaaagagt ggggcaagcg
    11461 cactccgaaa gagcgcgacc atttcgtgag cctgcttcga cagctcgtcg aacgccagta
    11521 ccagcgaaac atggaatcga cgctccaata caaggtctcc tgggtgggca ccgaacccat
    11581 cgagacgggc gtgaaagtga agtcgtcggc gaagtccgtc aaaaagaagc ggcagccgcc
    11641 gattacgatc gactacagca tgagccccgc cggcgaagag tggaaagtct ttgatatctt
    11701 caccgacgac gtgagcctcg tgaagaacta caagcgccag tttcgacgag tgatcgctga
    11761 cgagggctgg agcgggctga tcgaccgcat ggaaaagaag ctcaacgccg aagatgatct
    11821 gatctagcgc cggcgatcgg ggcacgggcg aacgcgacgc gggggttcaa cggacggggc
    11881 gccttgcaaa ggtggccgga aggactgcga gcaggggcca cgcaaggcgc ccctcggagc
    11941 tcgcgccaat cggagggggg ctgctcgctg accgattgtc actcgcacgc cgccgaaccc
    12001 ccacatcgcg ttcgcccgtg cccctccata tggagaagtg aatctcggcg cctgagtggg
    12061 aaacagttgg aggcccgtgg tggatcgggt cgcctttgat ccctgtgagc atcgccggcg
    12121 agtgtggccg ggatccattc aaagggaacg gcttctggtg ctgggcaaac gaattcgatc
    12181 cggttttcat cgactactgc gactgctgga caacctgcga cggagagcga gaccccaccg
    12241 aaatcaaatg cctctgatcc ttcaacggcg ctgacaccca gtctggcgga cgctccatat
    12301 ttcgcacgga ggggctcggg gcgacgcgat gcgggggttc ggcgacgtcg caagtgacaa
    12361 tcggcatggg agcaaccacc cccgattggc gcgcgcgccg aggcgaggct tgcgtggtct
    12421 aagctcgcca gtgcatccga ccacatctgc aagccgagcc gttcgtcgga cccccgtgtc
    12481 gtgtcgcccc gagccccgtc gttgcgctta ataaaaccgt ccgcccggct gcggcccccc
    12541 caggccgcgc gccgttcgga cgctcccctt tttctgttag accctcttcc gcagtcctgc
    12601 gatgtctttg atgacgacgc ggcgatgatc ggttgcgatc aagcctgccc ttttgaaggc
    12661 gccgagcacc aatgtgacgg tttccctcgt cgagccgacg tagctcgcga tttcctgatg
    12721 ggtgaacttc acaccgatca ggattccccg tgaatcggga ataccgtgcc tcgaggatgc
    12781 atcgagtaga aattctgcaa cacgcgattc tacgcttcgc gtgagcagtg catcgatccg
    12841 ccgttctgcg agcaggcggc gttcccccat taaccgatgc atagcggaag cgaactccag
    12901 gtcctcgctc atcaccattt tgatgaggcg cggcggaatg cgtagcgctt ccacataatc
    12961 caaaaccacc gtttcgagac gcgcttcggg gtcgaccagc gacatttcgc cgaccaggtc
    13021 gcccgggccg tagtagccga gcgtcagctc tcgttccggt gtgcggcgca cgcgccgaac
    13081 gcgaccactc ccgatcacga agatcgcggg gtcgctaggc atgcagaggg actcccgacg
    13141 atcggcgtcg accagagcac ttccactcaa gatgcgttct ctcagctccg aggtcagcgc
    13201 ctcgaaaatc ctgcagcgag ccatgattct cgagcggcgt tcttccgtgg tgcgcattcg
    13261 acgtgcgccg gagccgtttt gcattgcggg agcgttagaa tccatactta cagccttaac
    13321 ggtagattgc attaccgtag gattcccgtc aaggcgtatg ttcacaaaaa tacgcgaaat
    13381 aggaattttt cgttcttatt gaccgggagg gccgccttag ctagtgtcgc ggcattcaga
    13441 aagggatcgc tccggctcgg tgtcgaccga ctaaatatgc gactttactc aggaaaaatt
    13501 gggccgatag tcgacgactt ggtccgggag ctcacggcgc aggaagacat cgaggtcgag
    13561 gacgtcgccg aggttcggct agacctcgaa gcggtcctga aagagtacgt gcggcgcgag
    13621 cgtcagatgg tcgacgaggc gaaggatcgc atggagcgcg agggcctcgg gtactcgaag
    13681 ctcggccgca tgcgccagca gctcgccaag gagatgggct ttccgcagca agacgaggtc
    13741 cttccctacc tcgtcgacca gctcatcaac atgctgtttc acagctcaaa cgtcgtcgag
    13801 atctacgcgg acgacgtcac aatccgaaag aagatgaccc ccatcttgcg ccggcacatg
    13861 gaagtcgaaa cagggctcga cgccgaagtc cgctcgaaga tcaagaacct gcaagagggc
    13921 acttccgcgt tcgaggtcga gtacgcaaag atgatggatc agatcaagca caagcgcgga
    13981 ctcgagtagg cacctcatgc ggagcatgac tggctacgga agcgggcggg cagcgctcgg
    14041 ggatggtgag gtcgtgctcg acattcgaac ggtcaaccac cgcttcttgg acgtccgcgt
    14101 tcggcttcca tcacgaattc agactcgaac tccgatcgtg gagcgtgtga ttcgcaggcg
    14161 gctcgaacgc ggacgagtcg acgtgaccgc gcgcttcgaa gggcaaaccc tgccgcaacc
    14221 caggctggac ctcgaacgcg cccgcgcggt gtactcggag ctaaccgcgc tccgcgacga
    14281 gctcaacccc ggcgagccgg tgccactctc ggtgctgtct tcggtccccg acctgttcat
    14341 cgccgatcgg agcatcgatg atgaggccct cgatcgcgct ctcagcgtcg cggcggaggc
    14401 tgcttgcagc gccgttctcg ccatgcgcga gaaggagggc aatgccctgg catccgagct
    14461 gatcgaccgg ctatccgagc tcctcgcaaa cctcggcact ctctccgagg cggtgccatc
    14521 gatgctcgag gatcgcagat tgcgcctgcg tgatcgcctg gatgcgctct tggtcgacac
    14581 ccaggccgct ctcgacccct cccgtctgga gcaggagatc gccctcatgg cggaccgctc
    14641 tgatgtcgcg gaagagctcg tgcgcctcga gagtcatcgt gttcaaatgc tcgaactgat
    14701 cgagaattcc gacggagcgg ttgggaagcg tatcgacttt ttgctccaag agatggcccg
    14761 cgaagccaat acgatcggct ccaaggtgca ggatggagcg accacggcgc acgtcgtcgg
    14821 gttgaaggcc tgcatcgaga aaatgagaga acaagcacag aatgtgcttt gacccgcggc
    14881 gactgcgcgg tagtgctccg ttcgaagggg tagttccatg agcgtcgcag cgccgactga
    14941 ttcagatctc atcttgctga tcatcagctc tccgtcgggt gcgggaaaga cgacactgac
    15001 ccgccggctg ctgcaggaat tctccgattt caggttcagc gtctcgcaca ccacgcgtct
    15061 gccgcgcacc aacgaagtag acggccagga ctatcatttc gtcagcgaga gcgagttccg
    15121 gaacgtcatc gcgagcgacg ggttcgcgga atgggcagaa gttcacgcca acctctacgg
    15181 caccagtgtc gccgagatcg agctcgcacg agaggcggga aagcggggcg tcctcttcga
    15241 cgtcgactac caaggcgccc gtcagatcaa gaagaggttt ccgcacgccg taggcgtctt
    15301 cattcttccg ccttcgatga ccgagctccg gcgccgtctg gatgggcgtg gctccgatga
    15361 tgcagagtcg cgcaaacgcc gcttcgacaa ggcgcgtgat gaaatcggcc actacccgtt
    15421 cttcgactac atgatcgtca acgacgagct gcagcgggcc cttgccgagc tgcgcggcat
    15481 cgtcctcgcg gaaggctgca gacagtggcg cgtcgccgcg aaggcggagg ccctgcttca
    15541 agagtagcgt cagtacgcga taccgaggat gagcctcaat accattgcac ggctgatgcc
    15601 taggccttcg gtgcccggct cgctgatgcc gtcgacttgc agatactgga gcccgttcag
    15661 cgggaagaat atcccaaact gcgcggccgc gaagaagcca tccttgaagc tcgggccgtt
    15721 ttcggtccga tagtaaatgg agatatcgag ctccgcgccg aggtttggct cggaggagta
    15781 ggtctgctgc gcatacatcg cgcggctgta gatgaagtcc aagcgcgcgc cgaggacacg
    15841 tccgaagtcg gtgcggatga tgtcgtagct caccgcgggc gcgaggtagt acgcgcctgc
    15901 gactcggccc atgatgcttc gccagaagat caggtcgatg cggtagtcgg ggtgaaacga
    15961 gaagttcgtg acattcttct tgccaaccgg ctgggcggcc aggttctcat actgagaaag
    16021 gccgacgacg tcgggatcac ctgacgcagt tcctcccttg agaatcacgc cgagcttctg
    16081 ctggagaaac cggtactccc cctcgaatgc gaagccccac tggcgcaact tgtacggctc
    16141 gtcctcagca ctaggcggga atccgccggc tgtgatgttc tgaatctgtc cgaagatacc
    16201 cgcgaactcg atctccagtc gcaggttctt tcgcaacacc tgaacccagc cgtcggggat
    16261 gtagactttc gcgtcacggc gcacgaagct attctcgacg tcgctaatcg taccgatgag
    16321 tggagccgtc gaagtcgata ggaactggct gcggtagatg aaatatgcgc cgccgttgat
    16381 cacccatttg ccactggcaa tacgcttctc ctgggcaagc gcatccatgc gccgtgcgaa
    16441 gaggaagctc cattgacgcg tgtcgtccag cctgctggca tcgtatggga ttccctgaat
    16501 gttctcgacg ggatcgtaga tgtacccctt gttcgcgaag tcccaggaca ccccgaagaa
    16561 catccctttg aatttgccga tgagttgaat gcgatcgact tccgactgaa aatccgagtc
    16621 gagctcagtg tcgaggttgt tggtgccggt gcctgcgttc cacagcatcc cgagacccca
    16681 ttggttgccc atgcggccga atcgaagctg tccgatcgtg ctgttggtaa cctcgcccca
    16741 ggcacgtcgg acgatgatgt tgtcgccgac agtgtttcgg taggattcgg gtggcggcga
    16801 ggtcagcgtc aagctgtcga cgggaactcc gggcgcgcgg cctacgacct gaccgaactg
    16861 gttgacggac ttgacctcgg gcgtggaccc gagaaccaag ttgtcgaaca cgtcgatcat
    16921 catgtggacg cggatgttgt cactgggcgc cagagtcggc tgcagtcgca gtcgcatgtt
    16981 ggcgtatcgc agtgtcgcgg ttccgtcaca acgctcagcg ggcccagcta ccgaggtggg
    17041 tgggcccgtg cagccgcctt gcggcaggac gccgttgttg ccgttgctcg gcgaattgtc
    17101 gactggaatc caacgcacga acggatcatc tgggaagcgt gcgagcggct gccgtcgcag
    17161 cgagtagttg ttgacgtagt tgcctcgcac ccggaggtag ccgttgagag agaagatcgg
    17221 tcgcggattg gtccaggtgg ccaaagtcgg gtctgccgcc ggcggttcgg ctcgaccaag
    17281 gagcgcggcg tcgtccgaag ccatcttgtc ctcgtcgaaa tcggcctcat cgaactcatc
    17341 accggactcc tcgtcgctcg gcggagcgct aggcatctgc agcccttcga tgctcggcgc
    17401 gcgaagctgg ggggggaggt cttcctcgcg actgggctcg acctccgcgt cttcgacttc
    17461 ggagatcccc tcctgcgcct cggtttccga ggtcccctgg accccaggat cgctcacatc
    17521 gccggtggac gacctttgcg ccgcagccga tgttgccccc aagaaaacca gacaaatgat
    17581 cgcgaaaaac cggcgcattg aacccgtgcg gtcccgggcg tagcacgcag ggatcaggcg
    17641 ggtcaacgac gcaatcgctg ccttgaccgg agccacgccg gcctttagcc aggctgcagt
    17701 gacttcgatg tcggacacag tgcgcttcgt tgccctcggt gggctcaacg aagtggggat
    17761 gaactgcgcc gctctgacct gcggggacac gacgatcgcc atcgactgcg gcatcggttt
    17821 caccgatgaa catggtgccg agctggttca ccccaatttc gagtaccttt ttcaggaacg
    17881 cgagcgcctc cgggcgatcg tcatcacgca tgggcacgag g
//
LOCUS       DASRZF010000271         8914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2611846, whole genome shotgun sequence.
ACCESSION   DASRZF010000271 DASRZF010000000
VERSION     DASRZF010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 8914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8914
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2611846"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(295..984)
                     /locus_tag="VFG22_11995"
     CDS             complement(295..984)
                     /locus_tag="VFG22_11995"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HET6417011.1"
                     /translation="MVDPMGICEKHQLPLDRNGDCEMCRLSEMPSKAPPARSAWWALF
                     IPIALILGGIAWAFASIGSGPEVLPQRGVRDTPHQEGSAAPARRDRTDPEPEAIPTPP
                     PPSPQEINVPTPPRPESGELREDPRGQDPETKLTPDWKLDLARKRVQITMYATQWCGV
                     CREARAYLQANRIDFYEIDTDADSDANQRLGELNPRRTIPTFQIDELVYVGFRQETFE
                     AKLNQAARKHL"
     gene            complement(1085..2506)
                     /locus_tag="VFG22_12000"
     CDS             complement(1085..2506)
                     /locus_tag="VFG22_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET6417012.1"
                     /translation="MSTIGRRSFPRRLFLTGTAGVTLGLPFLETFASKMVRAASPADP
                     GFLVFMRQGNGCSQALPYSNPEEPEMFWPRNLGALTTASMLADGDRALSELSPHAENL
                     LLVRGVRGAFSISGCGHARGALQALTAQPTGDAEASNNSLALGESLDNRIARELNPDG
                     RGPLTLAAMRPSNFLDSVLSYRGPNDRRGAQTNPKVAFDDIMGLVELDESSANAIAAR
                     RSSVNDLVRDQMQALLNRSDLSSDDRNRLDLHFTAIRDLEIRLAECAPLDPSTVSTID
                     AIDGDHQNGDRIVEVAELQIDVIALALSCGYTHAVTLQIGNGNDGTRYWWQGEQFPSY
                     HWISHRIFADGSDGDPIPDAQAKHNAVDRIHARLFGRLIDRLSAYTTESGTLLDQGVC
                     AWINDLSNGPPHSINNLPYVLAGGAGGYLRTGQYVDAGDVPHNKLLNTLATAVGLRKA
                     NGDPVDDFGHASLENGLIDEMIA"
     gene            complement(2503..4176)
                     /locus_tag="VFG22_12005"
     CDS             complement(2503..4176)
                     /locus_tag="VFG22_12005"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HET6417013.1"
                     /translation="MWYRSWALCSLCIICACEGLIGSAANEAGSPAEPRCGRSVCGAT
                     GELAAGTAFPRLTHAQWENSVRDFLRVEDTPRLSGSFEPDTRVSFFDNNIRALRVSSD
                     LWSDYQSAAEALAEAATTDSAVLSNLLPSDLPSDPEERARATVRALGRRAYRRTLSNE
                     ETESHVALFEQGAGHFTEMAPFDAGVRMLLEAFLQSPHFLYRVEVGQGRTGDRVQLGD
                     FEIATRLSYMIWNTAPDDALLDEAERGALHTPEQIADQAVRLLNDARGQALLERFHAQ
                     LLNWDLFDDLSKDPSEFPLYYAGVGNDMYEEARSFVEHVVLEEDGNLAELLTAPYTFV
                     NADLAAIYGVDGAFDESFAKLDFDPSQQPQRGGLLTQLGFLASHGGLTGSVHRGVFIN
                     HRVLCTELPPPPDALPPIPADSGMTMTSRERIEMHTGPGTCGASCHGTFINPIGFAFE
                     SFDGLGQYRTEELGLPVDASSTFEFADGPVAFDGAAELNDAMSARMQTHDCYTKHWVE
                     YALGRDTAGSDLDLIQDLGDSSLGGLSIKKLIVELVRSEEFRVRNAEGS"
     gene            4289..5152
                     /locus_tag="VFG22_12010"
     CDS             4289..5152
                     /locus_tag="VFG22_12010"
                     /inference="COORDINATES: protein motif:HMM:NF044908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HET6417014.1"
                     /translation="MNQLTGLLAQLDLENLDVDLFRGSSPHDGRPRIFGGLVAAQSLV
                     AACRTIDGRAAHSLHGYFLREGDTSVPVIYHVDRIRDGRSFATRRVVAKQHGKAIFNM
                     SISFQAPEEGLDHQVAMPDAPPPESVPTNEERLRSYKERSTHPALDFLLLLDRPIQRR
                     EVDHVDLLNPMPRSGIQRTWFRADGSLGDDPVTHQAILAYASDYGLLEASFTEHGHSF
                     LTETLIIASLDHSMWFHQPFRVDQWMLYVTESPRASGGRGLNFGRIYTRDGALVASVA
                     QEGLMRIRKPR"
     gene            5149..8466
                     /locus_tag="VFG22_12015"
     CDS             5149..8466
                     /locus_tag="VFG22_12015"
                     /inference="COORDINATES: protein motif:HMM:NF024204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HET6417015.1"
                     /translation="MIRHYMPGRTLGVLGFCVSMLVLPPQALAQPSPLTGPSEPSAPG
                     ASPELAELERKAAELRARLAEWKAKSLEYLQAGSETQARAEAIDRDIEQLKRRDSILI
                     SEEASVAELSAQIVEAEEDLNAARREALELDSEAELRSERRKKIPELLSIAKQRLSEL
                     DNAPPRTSGDPAVAQALIDLASLRREVELAEIESYQRELASYEARGILLAKRRDRATL
                     RIAYYEFLGVKLREAKQRLERLAVERENDAAKRLLDELTALPDGVQKTLRDLYQRNAA
                     LASLWTDEGGLHDQIADVRDKLFRAEEKVVEVEAELTRLAARVEAVGLADSVGVLLRR
                     RRAEAPDIGMYRRFIRMRQEQIGSVQLQQIKLREQRNALADIDTLVAQVMASIEEPIP
                     RGERAEIEDLLRKLFVTQRMYMDALIEDYDTYFEKLVDFDSMQRELIARTQDLLDFID
                     QRILWIPSGKAVQPELLSDGRNAMVWLFSPTHWAQLVKAFRDAATRSWPLNLLALLFA
                     IGFVPFARRVRPRIRSLGQEAKQSNCTLFGPTAETLGLTVLLAVWLPAFVAYVCWRLG
                     LSPASTQFSRSIAHGLLVASGFWASLRLVRQIVRPGGLAEAHCGWPEKTAHAMWGNLR
                     WLTAITVPAIFVVFVFEMRGEDLWRESVGRLAFLIAIFATVVFNHLALRSGGPLADLF
                     HARSLRRPWIWRVIQLAAVLVPLLLAAVTLRGFYWTALQVAIRMHLNFGFLFLLVIVI
                     QLAARWTLVAERRALIEEEAVEERSSRLAPTLEVSRLMIGTGVLLAILGSLAIWADLL
                     PAAKILEQVELWSSTQTVTVSELDASGMERVSTEDRLVAITLADLFRAFLIGLLSLAL
                     IRALPGILEATLFRRLGPGERYAYATIVEYTVAICGVGLAFDAIGVGWSSIQWLVAAV
                     GLGLGFGLQEIFANFVSGLIILFERPIRVGDTVTVGGVNGTVTKIQIRATWITAFDRK
                     ELVVPNKEFVTGQLINWTLSDAILRVDIPVGIAYGSDTDLAIEVLERVARESNRVLAD
                     PRPQVMFTSFGDSSLSFELRVYIRTVAQLFPTRHELHMAVDKAFREAGIEIAFPQRDL
                     HLRTVPENWKPPSDRSR"
     gene            complement(8483..>8914)
                     /gene="gspC"
                     /locus_tag="VFG22_12020"
     CDS             complement(8483..>8914)
                     /gene="gspC"
                     /locus_tag="VFG22_12020"
                     /inference="COORDINATES: protein motif:HMM:NF041515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HET6417016.1"
                     /translation="KPALGRAQSRRNAAGPQSDRNAGLSDEEMEEGIERINDSNFNLS
                     RSLLNKVLDNAGKLIGIAAVRPKIEDGQSVGMEMGGVRPGTLLTKIGLQNGDVLESIN
                     GQPLSSPDTALGAYTTLRTADKFNLSIRRGGQPMTINYNLQ"
BASE COUNT         1608 a         2693 c         2781 g         1832 t
ORIGIN      
        1 accaccagtg cgtggcgagt cggtgccgcg aaaacatgct cgtagcaggc ctgcatgtac
       61 tcgatgtcgg atgtcgtcgt gatcggcggc attgaaatga gaacgagtgg ccacattgaa
      121 acatcgaacc caagccccac ggcggctggc cgtcccgcaa tcctcggcgc gttccccatg
      181 gcgcgattat cggcattgcg gctgtcgaag caaactaggc gccaatctct gagccgagct
      241 gccgttttcg aagggcaatt ccccggattc gtggcaccgg atccgccgct agcgctacag
      301 atgctttcgc gcggcctgat tcaacttcgc ttcgaaagtc tcctgccgaa atccaacgta
      361 caccaactcg tcgatctgga aggtggggat cgttcggcgg ggattcagtt ccccgaggcg
      421 ctgattcgcg tcgctgtcgg cgtccgtatc gatctcgtag aagtcgattc ggttggcctg
      481 gaggtacgcc cttgcctctc ggcagacgcc gcaccactgg gttgcataca tcgtgatttg
      541 gacacgcttg cgagcaaggt ccaacttcca atccggcgtc agtttcgttt cggggtcttg
      601 ccctctggga tcttcgcgaa gctcgccact ttcgggacgc ggtggcgtgg gcacgttgat
      661 ttcttggggg gagggtggcg gtggcgtcgg aatcgcctcg ggttcgggat ccgtccggtc
      721 tcttcgagcg ggtgcagcgc tcccctcctg gtgtggcgta tctcgaactc cacgctgcgg
      781 aaggacttcc ggtccggatc cgatcgaagc gaacgcccaa gcaatacctc caaggatgag
      841 ggcgatcggg atgaatagcg cccaccaagc gcttcgtgcg ggcggcgcct tggagggcat
      901 ctcgctcaac cggcacatct cgcaatcgcc gtttctatcg agcggaagtt gatgcttttc
      961 gcaaatcccc atggggtcca ccatctgagt ttcagtctat cacgatcgtg aatcaggctc
     1021 agcgatgtag ccaggtacgc cgctgttacc agtccggccg gagcggcgcg atcgcgggtc
     1081 caatttacgc aatcatctcg tcgattaggc cgttctccag gctggcatgg ccgaagtcat
     1141 ccacgggatc gccgttcgct tttcgcagcc caaccgccgt cgcaagcgtg ttgagaagct
     1201 tgttgtgggg aacgtccccc gcgtcgacgt attgcccggt acgaaggtag ccaccggcac
     1261 cccctgcgag cacgtacggc aggttgttga tcgagtgtgg cggcccgttc gagagatcgt
     1321 tgatccacgc gcagacgccc tggtcgagca gtgtgcccga ttctgtcgtg taggccgata
     1381 ggcgatcgat gagtcgaccg aacagacgtg cgtggatccg atccacggcg ttgtgcttgg
     1441 cctgagcatc cgggatcgga tcaccgtctg aaccgtcagc gaaaatccga tgtgagatcc
     1501 agtggtacga aggaaactgc tcgccctgcc accagtatcg cgtgccgtcg ttgccgtttc
     1561 cgatctgaag cgtcaccgca tgcgtgtagc cacaggatag agccagcgcg atgacgtcga
     1621 tctgaagctc cgccacttcg acgattcgat cgccgttctg gtggtcaccg tcgatcgcat
     1681 cgatcgtgct caccgtcgat ggatcgagcg gcgcacattc ggcgaggcga atctcgagat
     1741 cgcgaatcgc cgtgaagtgc aggtccagtc ggtttcggtc gtccgagctg agatcgctgc
     1801 gattcaagag tgcttgcatc tggtcgcgca cgagatcgtt gacactcgat cggcgcgctg
     1861 cgatggcgtt ggccgacgac tcgtcgagct cgaccaggcc catgatgtcg tcgaaggcga
     1921 ccttgggatt cgtctgggcc ccgcgccggt cgtttggccc gcggtaggaa agcacgctgt
     1981 cgaggaagtt ggacggccgc atcgccgcga gcgtgagagg tcctctaccg tctgggttga
     2041 gctctcgcgc gatgcggttg tcgagtgatt cgccgagcgc gagcgagttg ttgctagctt
     2101 ccgcgtctcc ggttggctgc gccgtgagcg cctggagcgc accccgggca tgaccgcagc
     2161 cggatatcga aaacgctccg cgcacacctc gcacaagcag aagattttcc gcatgggggc
     2221 tcagctcgct cagtgcccga tcgccatcag cgagcatcga agcagtggtg agcgcgccga
     2281 gatttctagg ccagaacatc tcgggttctt ccgggttgct gtaagggagc gcttgagagc
     2341 agccgttgcc ctggcgcatg aacacgagga aacccggatc ggccgggcta gcggcgcgca
     2401 ccatcttcga cgcaaaggtc tccaggaagg gaagcccgag ggtcaccccg gcggtgccgg
     2461 tgaggaatag gcggcgcgga aaggagcgtc tgccaatcgt gctcatgagc cctccgcgtt
     2521 gcgcactcga aactcttcgc ttcggacgag ctccacgatc agcttcttga tcgacagccc
     2581 accgagggac gaatcgccca gatcttgaat gaggtcgaga tcggatccgg cagtgtcgcg
     2641 gccaagcgcg tattcgaccc agtgtttggt gtagcagtcg tgcgtttgca ttcgcgcaga
     2701 catcgcgtcg ttgagctcag cggcgccgtc aaaagccacc gggccgtccg cgaactcgaa
     2761 cgtcgacgag gcgtccacgg gaagtccgag ctcttcggtg cggtattggc ccaaaccgtc
     2821 gaagctctcg aatgcgaacc cgattggatt gatgaatgtt ccatgacagc tggcgccgca
     2881 ggtcccggga ccagtgtgca tctcgatgcg ctcccgcgat gtcatggtca tgccgctgtc
     2941 tgcaggaatt ggaggcagcg cgtcgggtgg tggaggtagc tcggtgcata ggacgcggtg
     3001 attgatgaag acgcctcggt ggaccgagcc agtgagccca ccgtgggagg cgaggaatcc
     3061 cagttgggtc agcaatccgc cgcgttgagg ttgctgcgac ggatcgaaat ccagctttgc
     3121 aaacgattcg tcgaaggcgc cgtcgacgcc ataaatcgcc gctagatccg cgttgacgaa
     3181 tgtgtagggc gccgtcagta gctccgccag attcccgtcc tcttcgagta cgacgtgctc
     3241 cacgaaagat cgagcctcct cgtacatgtc gttgccgacg cctgcgtagt agagcggaaa
     3301 ctcggacggg tccttgctga ggtcgtcaaa gagatcccag ttcaagagtt gagcgtggaa
     3361 gcgctccagc aacgcctgcc cccgcgcatc gtttagcagg cgtactgcct ggtcggcgat
     3421 ttgctccggg gtgtgcagcg cgccgcgttc ggcttcgtcg aggagcgcgt catcaggcgc
     3481 ggtattccag atcatgtacg agagtctggt cgcaatctcg aagtcaccga gctgcactct
     3541 gtccccagtc cggccttggc cgacctctac gcggtacaag aagtggggcg attgcaggaa
     3601 ggcttcgagc agcattcgga ccccagcatc gaagggtgcc atctctgtga agtgtccggc
     3661 gccttgctcg aacagtgcta cgtgcgattc ggtttcctcg ttcgaaagcg tgcggcggta
     3721 cgcgcgacgg cccaaggcgc gaaccgttgc gcgtgctcgc tcctccgggt cgctcggaag
     3781 atcgctcgga agcaagttgc tcagcaccgc gctgtccgtc gtagccgcct cggccaaagc
     3841 ttcggctgca ctctggtagt cgctccagag atcgctgctc acccgcagcg cccggatatt
     3901 gttgtcgaag aagctgaccc gggtgtccgg ttcgaagcta ccggagagcc tgggggtgtc
     3961 ttccacgcgc agaaagtcgc gaacgctgtt ctcccactgg gcatgtgtca gccgaggaaa
     4021 cgccgtgccg gcggcgagct ccccggtcgc tccgcagacc gagcgaccac agcggggctc
     4081 tgccggcgag cccgcttcgt tcgcagcgct gccgatgagg ccttcacagg cgcagatgat
     4141 gcacagagag caaagtgccc aggagcgata ccacattcga ttaaccctaa gggatcataa
     4201 ttgcatccta aagggtgtgg gtgtcaagga gccaatcggt actcagcaat gggtccagca
     4261 tctggatatc ccgtgctaat tcacttcggt gaaccagctc accggccttc tcgcgcagct
     4321 cgacctggag aacctcgatg tcgatctgtt tcgtgggagc agccctcatg atggccgacc
     4381 tagaatcttc ggcggtctcg tcgcggccca gtcactcgta gccgcttgcc gcaccatcga
     4441 tgggcgcgcc gcgcattcgc tgcacgggta cttcctgcgc gagggtgata cgtcggtacc
     4501 ggtcatctac cacgtcgatc ggatccgcga tggacgttcg ttcgcgacgc ggcgcgtggt
     4561 ggcaaagcag cacgggaagg cgattttcaa catgtcgatt tccttccagg cgcccgagga
     4621 gggactcgac catcaagtgg ccatgcccga tgcgccgccg cctgagtccg ttcccacgaa
     4681 cgaagagcgt ttgaggtcgt acaaggagcg atcaacgcat ccagcgcttg attttctctt
     4741 gttactcgac cgtccgattc agcgtcgcga ggtcgatcac gtcgacctct tgaacccgat
     4801 gcctcgcagc ggcatccaac gcacttggtt tcgcgcggac ggctccttgg gcgacgaccc
     4861 cgtcacccac caggcgatcc tcgcgtacgc gagcgactac ggcttgttgg aggcgtcgtt
     4921 caccgagcac ggccacagct tcctgaccga gacgttgatc atcgccagcc tcgatcattc
     4981 gatgtggttt caccagccat tccgcgtcga ccagtggatg ctctacgtca ccgaaagccc
     5041 acgagcttca ggcggtcgag ggctcaattt tggccgaatt tacactcgag acggcgcact
     5101 cgtcgcttcg gtggcacaag aggggctgat gcgtattcgg aagccacgat gattcgtcac
     5161 tacatgccag gccgcacgct cggagtactg ggtttctgcg tgtcgatgtt ggtcctgcct
     5221 ccccaggcgc tggcacagcc aagtcctttg accgggccct cggaaccaag cgccccggga
     5281 gcttctcccg agttggccga gctggaacgc aaggctgccg agctgcgcgc aaggctcgcg
     5341 gaatggaagg ccaagtcgct cgagtacctc caggcgggaa gcgaaacgca agcgcgtgct
     5401 gaggccatcg atcgagacat cgagcagctc aaacggcggg attcgatcct gatctccgag
     5461 gaggcaagcg ttgcggagct cagtgcgcag attgtcgagg ccgaagaaga tctgaatgcg
     5521 gcgcgacgag aggccctcga gctcgactcc gaggctgagc ttcgatctga acggagaaag
     5581 aagattccgg agctgctttc gattgccaaa caacggctga gcgagctcga caacgctccc
     5641 cctcgaacta gcggcgaccc cgcggttgct caagcgctca tcgaccttgc ttcgctaaga
     5701 cgagaggtgg agctggccga aatcgaatcg taccagcgag agctcgcaag ctacgaggcg
     5761 cgcggaatct tgctcgcaaa gcggcgcgac cgagcgacct tgcggatcgc ttactacgaa
     5821 ttcctgggcg tcaagctgcg cgaggcgaaa cagcgattgg agcggcttgc ggtggagcgc
     5881 gagaacgatg cggctaagcg gctcctcgac gagctaaccg cgctccccga cggagtacag
     5941 aagacactcc gagatctcta tcagcggaat gcggcgctcg cttcgctctg gaccgacgag
     6001 ggcgggcttc acgatcagat tgcggatgtc agagacaagt tattccgcgc tgaagagaag
     6061 gtcgtggaag tcgaggccga gctcaccagg ctcgcagcgc gcgtggaggc tgtgggtctg
     6121 gcagattcgg tcggcgttct gttgcggcgt cgtcgggcag aggctccgga tatcgggatg
     6181 tatcgccggt tcattcggat gcgccaggag cagatcggga gcgtacagct acagcaaatc
     6241 aagctcagag agcagcggaa cgcgctagcc gacatcgaca ccctggtagc ccaagtcatg
     6301 gcgtccatcg aggaaccgat tccccgcgga gagcgcgcgg agatcgaaga cctgcttcgc
     6361 aagcttttcg tgacgcagcg catgtacatg gacgcgctga tcgaagacta cgacacctat
     6421 ttcgagaagc tcgtcgactt cgattccatg cagcgcgagc tcatcgcgcg tactcaggat
     6481 cttctcgact tcatcgatca gcgaattctc tggattccga gcggcaaggc ggtccagccc
     6541 gagcttctat cagacggacg aaacgccatg gtttggcttt tcagcccgac gcattgggcg
     6601 cagctcgtca aagcatttcg cgacgcggct acccgttcct ggccgctgaa cttgctcgca
     6661 ttgttgttcg cgattggatt cgttcctttt gcccgacgcg tgcggccaag gattcgatcc
     6721 cttgggcagg aagcgaagca atccaactgc accctgttcg gtccaaccgc cgagacgctc
     6781 gggctcacgg tcttgctcgc tgtttggttg ccggccttcg tggcctacgt ttgctggcgg
     6841 ctcggcctgt cgcccgcgtc cactcagttc agtcgaagca tcgcacatgg cttgctggtc
     6901 gcttcggggt tctgggcatc acttcgactg gtacgtcaga tcgttcgtcc aggcggtctt
     6961 gcggaggcgc attgcggctg gccggagaag accgctcacg ccatgtgggg gaatctacgc
     7021 tggctcacgg cgatcacggt tccggccatc ttcgtagttt tcgtcttcga aatgcgcggg
     7081 gaggacctct ggcgcgaatc cgtcggcagg ctcgcgttct tgatcgcgat cttcgcgact
     7141 gtcgtcttca accacctggc ccttcgatcg ggcggcccgc tcgccgactt gtttcacgct
     7201 cggagcctgc ggcgcccctg gatctggcgc gtgatccaac tggctgcggt gctcgtgcct
     7261 ctcctgcttg cggccgtgac tctgcgcggg ttctattgga cagcgcttca agtcgcgatc
     7321 cggatgcatc tcaatttcgg attcctgttc cttctggtca tcgtgattca gctcgccgcg
     7381 cgctggacgt tggtcgcgga gcgacgagct ctgatcgaag aagaggcggt cgaagaaagg
     7441 tcgagcaggc tagcgccgac cttggaggtc agccgtctga tgatcgggac gggcgtcctt
     7501 ctcgcgatcc taggctcctt ggcgatctgg gcggatctat tgcccgctgc gaaaatcctc
     7561 gagcaggttg agctttggtc gtccactcag acggtaaccg tttccgagct cgacgcgtct
     7621 ggcatggagc gcgtttccac cgaggaccgc ctcgttgcaa tcacactcgc cgatctcttc
     7681 cgtgccttcc tgattggctt gctgagcttg gcgctgattc gcgcgttgcc cggaatcttg
     7741 gaagccacct tgtttcggcg tcttggtccc ggtgaacgct atgcgtacgc caccatcgtg
     7801 gaatacacgg tggcgatatg cggagtcggg ctcgcatttg acgcgatagg tgtgggctgg
     7861 tccagcattc aatggctcgt ggctgccgtc ggcctcgggc tggggttcgg cctccaagag
     7921 attttcgcca atttcgtttc cgggctcatc atcttgtttg agcgaccgat ccgcgtaggc
     7981 gacaccgtga ccgtgggcgg cgtcaatgga acggtcacca agatccagat ccgagcgact
     8041 tggatcacgg ccttcgatcg caaagagctc gtggtgccga acaaggaatt cgtgactggc
     8101 cagttgatca actggacgct gtccgacgcg atcttgcgcg tcgatatccc cgtgggcatc
     8161 gcttacggtt ctgacaccga cctcgccatc gaggttctcg agcgtgtcgc ccgtgagtcg
     8221 aatcgggtcc tcgcggatcc gcggccgcaa gtcatgttca cgagcttcgg tgacagctcg
     8281 ctttctttcg agcttcgcgt ctacattcgg accgtcgcac aattgttccc cacacggcac
     8341 gagctgcaca tggccgttga caaggctttc cgcgaggccg gaatcgagat cgcgttcccg
     8401 caacgcgact tgcacctgcg aaccgttccg gaaaattgga aaccgccgtc agaccgctcc
     8461 agatgagtcc cgcgacgcga gcttactgca agttgtagtt gatcgtcatg ggctgcccgc
     8521 ctcgccggat cgacaggttg aacttgtctg ccgttctcag ggtggtgtag gcgcccaaag
     8581 cggtgtcggg gctcgacaaa ggctgaccgt tgatgctttc gaggacgtcg ccgttttgga
     8641 gcccgatctt ggtcaacagc gtgccgggtc gaacgccccc catttccatg cccacggatt
     8701 gaccgtcttc gatcttggga cggaccgccg cgattccgat gagcttccca gcattgtcga
     8761 gcaccttgtt gagcaaggat cgagagagat tgaagttgct gtcgttgatc ctctcgatgc
     8821 cttcctccat ctcctcgtcg ctcagcccag cgttccgatc cgattgcggg ccggccgcgt
     8881 tgcgccggct ctgggccctt ccaagagcag gctt
//
LOCUS       DASRZF010000272         2605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_5013766, whole genome shotgun sequence.
ACCESSION   DASRZF010000272 DASRZF010000000
VERSION     DASRZF010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5013766"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..578
                     /locus_tag="VFG22_12025"
     CDS             27..578
                     /locus_tag="VFG22_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417017.1"
                     /translation="MTRPLIASFLAHLDGQASREVALQAYREAEGKHGVRIYDDHDAR
                     VAPGAFKPNMPISEERVRTLTRSWEQFDRALEPLGRADERNLVIRSLGPFLSDTGLWI
                     AGADLAHDHLFIFRSGEVGSWTAEQWDVHLANWAAVTQYRGRDDWTPERLVEQVRKAP
                     RYEEWTTLLRGLIAEARGAVLRS"
     gene            complement(579..1178)
                     /locus_tag="VFG22_12030"
     CDS             complement(579..1178)
                     /locus_tag="VFG22_12030"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HET6417018.1"
                     /translation="MRIEIHPTHPQARRVQQAVEVLRRGGVIVYPTGTVYGLGCDIHQ
                     KKAIERIYQIRRLKKDHPLSFMCPDLSKIARYAHVDDFAYRIMKRLVPGPYTFVLSAT
                     REVPKLLVRKQKTVGIRVPDDEIALALLNELGSPIVSTSATLDGEVLNDPDELYARFA
                     HADAFVDGGWGGIEPSTIIDLSGGEAIVLRQGAGPIDAL"
     gene            1273..1779
                     /locus_tag="VFG22_12035"
     CDS             1273..1779
                     /locus_tag="VFG22_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417019.1"
                     /translation="MTEERDPRPYVLITVLLDSSARPAEISRSHGDAYERSLIASEGQ
                     EIAGLELVELPIAAPVFKALRQPLAVPGDAVGLYDVFPLASHLKPEYRKIAGQFLAAE
                     ALWTMEEQGLLGGVPVNVKLEVPKGWKTDPKDIHQHLISEGALDLSPAGIETYKVIKT
                     AWDSRNAR"
     gene            complement(1805..2206)
                     /gene="apaG"
                     /locus_tag="VFG22_12040"
     CDS             complement(1805..2206)
                     /gene="apaG"
                     /locus_tag="VFG22_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HET6417020.1"
                     /translation="MSPPRTRSRAETRGVVVEVESQYIPDQSHPAMRHFVFSYTITIT
                     NRGSEVVQLRSRHWIITDGLGDVEEVKGPGVVGEQPVLSPGEGFRYTSGAVLSTERGM
                     MHGTYQMHRADGARFDVTIAPFLLERPYSLN"
     gene            complement(2203..>2605)
                     /locus_tag="VFG22_12045"
     CDS             complement(2203..>2605)
                     /locus_tag="VFG22_12045"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HET6417021.1"
                     /translation="HAYVYLCYGMHQMLNLVTNRDGEGAAVLIRSCEPIAGHGTIRRR
                     RGGKQGPVLLTGPGKVGQALGLDPGFSGHPLYRRGGLEVRKGQTASQVLVGARIGIGY
                     AQSEHQEAEWRLAIAGSPWVSHARELRSAQR"
BASE COUNT          473 a          836 c          825 g          471 t
ORIGIN      
        1 gagccgccaa ctagatggaa cgcacagtga ctcgtccact catcgcctca ttcctcgccc
       61 atctcgacgg tcaagcatcg cgtgaagtgg ctcttcaggc atatcgcgag gccgagggga
      121 agcacggtgt ccgcatctac gacgaccacg acgcacgtgt cgcccccggt gcattcaaac
      181 ccaacatgcc gatttcggaa gaacgcgtgc gcacgctgac ccgaagctgg gaacagttcg
      241 accgggccct cgagccgctt ggacgcgccg acgagcggaa tctcgtcatc cgttctctcg
      301 ggccgttcct gtccgatacc gggctgtgga tcgccggcgc tgaccttgcc cacgatcacc
      361 tgttcatctt tcgatccggc gaggtcggct cctggacggc ggagcaatgg gacgtgcatc
      421 tcgcgaactg ggccgcggtc acgcagtaca gggggcgcga cgactggacc cccgagcgct
      481 tggtcgagca agtgcgcaag gctccgcgct acgaagaatg gacgacgctt cttcgcggcc
      541 tgatcgccga agcgcgcggc gccgtgctgc gcagctagtc aaagagcgtc gatcggccct
      601 gcgccctggc gcaaaacgat cgcttcgccg ccgctgaggt cgatgatcgt cgagggctcg
      661 atgccgcccc atcccccatc gacgaaggca tctgcgtgcg cgaagcgggc gtagagctcg
      721 tctgggtcat tgagcacctc tccgtccagc gtcgccgatg tcgaaacgat cgggctcccg
      781 agctcgttga gcaaggcgag cgcgatctcg tcgtcgggaa cgcgaatccc caccgtcttc
      841 tgctttcgca cgagaagctt gggcacctcg cgtgtagcgc tcagtacgaa ggtatagggg
      901 ccgggcacca gtcgcttcat gatccgatac gcgaagtcat cgacgtgtgc gtagcgggcg
      961 atcttgctga gatcgggaca catgaagctc agcggatgat cctttttgag gcggcggatc
     1021 tggtagatgc gctcgatggc ctttttttga tgaatatcgc agccgagccc atagacggtg
     1081 cccgttggat acacgattac cccgccgcgg cgcagcactt cgaccgcttg ctggacccgc
     1141 cgggcttggg gatgagtcgg gtggatctcg atgcgcattg ggccgcccgt cggggtagcg
     1201 atccgccagc gtggcgtcaa ggcgacgagc actacccaag cggtgccaaa tgcgctactt
     1261 agtcgcgcgc taatgaccga agagcgagat ccacgcccat acgtactgat tacggtgctt
     1321 ctagatagct ccgcaaggcc ggctgagatc agccgaagcc acggggacgc ctatgagcgt
     1381 tcgctcatcg caagcgaggg gcaggagatt gcgggcctcg agctcgtcga gttgccgatc
     1441 gccgcgccgg tgttcaaggc cctgcgtcag ccgctcgccg tacccggaga cgcggtcgga
     1501 ctttacgatg tgtttccttt ggcgagccat ctcaagcctg agtaccgcaa gatcgcggga
     1561 cagttccttg cggccgaagc gctttggacg atggaggagc aaggcctcct cggcggagtt
     1621 ccggtcaacg tgaagctcga ggttccgaag ggctggaaga ccgacccaaa ggacatccac
     1681 cagcacctga tcagcgaagg cgcgctcgac ctgagcccgg ctggcatcga gacctacaaa
     1741 gtcatcaaga ccgcctggga cagccgaaac gcgcgttgat cgcaggcggc tgctctcggc
     1801 cgctctagtt cagagagtac ggtcgttcca agaggaaggg agcgatggtg acatcgaatc
     1861 gagcgccatc tgctcgatgc atttgatagg tgccgtgcat catgcctcgc tcggtcgaga
     1921 gcaccgcgcc gctggtgtaa cggaaccctt cgcccgggct cagaaccggt tgctctccga
     1981 cgacccccgg tcccttgacc tcttcgacgt cgcccaggcc atcggtgatg atccaatggc
     2041 gacttcgtag ctgaacgact tcgctgcccc gattggtgat cgtgatcgtg tagctgaaca
     2101 cgaagtgccg catcgccggg tgcgattgat cggggatgta ctggctctcg acctcgacga
     2161 cgacaccgcg ggtttcggcg cgcgaacgtg ttcgtggcgg gctcatcgct gagcgctccg
     2221 aagctcgcgg gcatgactca cccagggcga gccggcgatc gccagccgcc attccgcttc
     2281 ttgatgctcc gactgggcgt agccgattcc gatgcgcgcg ccgacgagca cctggctcgc
     2341 cgtctgtcct ttgcggacct cgagcccacc gcggcgatac aagggatgcc cactgaaccc
     2401 cgggtcgaga ccgagcgcct ggccgacctt ccccggaccg gtgagcagca cgggtccctg
     2461 cttgccgccg cgtctgcggc gaatcgtgcc atgccctgcg atcggctcgc aggagcggat
     2521 caagacggcc gcgccctccc cgtcccgatt ggtcaccaag ttcagcatct gatgcatgcc
     2581 gtaacacaga tacacgtagg cgtgg
//
LOCUS       DASRZF010000273         2568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1234187, whole genome shotgun sequence.
ACCESSION   DASRZF010000273 DASRZF010000000
VERSION     DASRZF010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1234187"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VFG22_12050"
     CDS             complement(<1..345)
                     /locus_tag="VFG22_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417022.1"
                     /translation="MQGALPAKLMKPLTWYTGDATYDTVLTLAFVLVALVFIGAAIVK
                     SPYGRFASNKWGLNLSPRLGWFLMELPATLSFVFFYFQGRNRFEIVPLFFLLVWLIHY
                     GNRGFVFPYLIRS"
     gene            complement(318..797)
                     /locus_tag="VFG22_12055"
     CDS             complement(318..797)
                     /locus_tag="VFG22_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417023.1"
                     /translation="MPTETMSAAAEQTKPKYLEIDHPWVSHRHVPIYEWAFPAEASDE
                     ELAGFIRAREDWASRAHYPVAWVVELSNLTTASAKQRRAFAEHLQRFEPHDVLWNGGS
                     ALIVPSAWLRGLVTAVFWISPPKFPHQAFAKRSDALEWAQLQLDAKLAECRAHSPQS"
     gene            869..1246
                     /locus_tag="VFG22_12060"
     CDS             869..1246
                     /locus_tag="VFG22_12060"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET6417024.1"
                     /translation="MAEDLKTMRELEDLMSEDGGAAIIDFWSPTCGPCKAMAPAFEAL
                     SQELAEEPIRFVRINTAEQPALSQPFHIHSVPTLLFVNDGEILDVRVGALPGPQLAKK
                     AQWLLRKARGEGFFSGLFGGKQT"
     gene            1243..>2568
                     /locus_tag="VFG22_12065"
     CDS             1243..>2568
                     /locus_tag="VFG22_12065"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HET6417025.1"
                     /translation="MKVRATAAPGLLLVSLLLSVLGCTEPVPLGSVGGSGGEGGAGGV
                     GGVEVKSERFIRDADGRALILHGLNIENGAKSDPLRVGSTTREFIVHMKRAWGFNASR
                     HLIFWDAIEPEQGVYDEAYLDRLEERLDWYAEAGVFAILDMHQDVYSAFFCCDGAPEW
                     AVRTDGIPYEPREIWWANYFEPAVQRTFDNFWDNEGPHADVQESYVAAWLHVIERFRD
                     HPAVLGYDLMNEPDGGSRLRTEFEQTVLPAFYDRLIARIREVDPDGWIFYEPTAFIVN
                     PGGPSELGPVQDRRTGERRLVYAPHFYDPTLFLNGDYQGPAAIENWEANRGAEIEERH
                     PGPLLIGELGAGPPAYHHDVLAMADRIWAGWTRWSSDSFFEAFEDGREPDDILDMVRV
                     YPQRIAGDPTDFGYNVAKRAFSLRFSERDGVEGPTEIYIPEARVYPDGWR"
BASE COUNT          503 a          786 c          813 g          466 t
ORIGIN      
        1 actccgtata agatagggaa agacgaagcc gcgattgccg tagtggatga gccagaccag
       61 caagaagaag aggggcacga tctcgaagcg gttgcggccc tggaagtaga aaaagacaaa
      121 gctgagggtc gctggaagct ccatcaggaa ccagccgagg cgcgggctga gattgagccc
      181 ccatttgttc gaggcgaagc gcccgtaggg gctcttgacg atcgctgcac cgatgaagac
      241 caacgcaacg agcacgaacg ccaacgtcag taccgtgtcg tacgtcgcgt ccccggtgta
      301 ccaggtcaac ggcttcatca gctttgcggg gagtgcgccc tgcactcggc aagcttcgca
      361 tcgagttgaa gctgcgccca ttcgagcgca tccgagcgct tggcaaaggc ctggtgaggg
      421 aacttcggag gcgaaatcca gaacactgcg gtgaccaggc cacgaagcca ggcgctcggc
      481 acgatcagcg ccgagccccc attccagagc acgtcgtgtg gctcgaaccg ttgtaggtgc
      541 tcggcaaaag ccctgcgctg cttcgcgctg gccgtcgtga gattcgaaag ctcgacgacc
      601 caggcgaccg ggtagtgggc ccggcttgcc cagtcctccc gcgcgcggat gaacccagcc
      661 agctcctcat cgctcgcttc ggccggaaaa gcccactcgt aaatcggcac atgcctgtgc
      721 gaaacccagg gatggtcgat ctcgaggtac ttcggcttgg tttgctcggc ggcggcggac
      781 atggtttcgg tcggcatgat agccctaaac ggcctcgctg ggcatcggat atctggcgcg
      841 aggcttccgg aaatgcgaga gaaatgagat ggctgaagac ctcaagacca tgcgtgagct
      901 cgaagacttg atgagcgagg acggcggcgc cgcgatcatc gacttctggt ccccaacctg
      961 cggaccctgc aaggcgatgg cgcccgcctt cgaggcgctt tcgcaggagc tcgccgagga
     1021 gccgatccgc ttcgtgcgga tcaacaccgc cgagcaaccg gcgctctccc aaccgttcca
     1081 catccactcg gtaccgactt tgctcttcgt caacgacggg gagatcctag acgtccgcgt
     1141 tggggcgctc cctggccctc agcttgccaa gaaggcgcaa tggctccttc gtaaagcgcg
     1201 cggcgagggt ttcttttcgg gccttttcgg cggaaagcag acatgaaggt gcgagcgacc
     1261 gcggcgccag gactcctcct ggtctcattg ctcctgtcgg tgctcggttg caccgagcca
     1321 gtgccccttg gttccgtggg gggtagcggc ggcgagggcg gagccggggg cgttggcggc
     1381 gtcgaagtca aatccgagcg attcatccgt gacgcggatg gtcgagcgct gattctccat
     1441 ggcctcaaca tcgaaaatgg ggccaagtcg gacccgctcc gggtcggatc caccacgcgc
     1501 gagttcatcg tgcacatgaa acgggcctgg ggattcaacg cgtcgcgaca tctgatcttt
     1561 tgggatgcga tcgagcccga gcaaggcgtc tatgacgaag cctatctcga ccgactcgaa
     1621 gagaggctcg actggtacgc cgaagccggg gtctttgcga tcctcgacat gcatcaggac
     1681 gtctactctg cgttcttttg ctgcgatgga gccccagaat gggcggttcg tacggatggg
     1741 attccttacg agccgaggga gatctggtgg gcgaactact tcgagcctgc cgtccagcgc
     1801 acattcgaca acttctggga caatgaaggc ccccacgccg atgtgcagga atcttacgtg
     1861 gctgcctggc tgcacgtgat cgagcgcttc cgagatcatc ccgccgttct cggctacgac
     1921 ctgatgaacg agcccgatgg aggctcgagg ctccgcaccg aattcgagca aacggtgctc
     1981 cccgcgttct acgatcgatt gatcgcccgc attcgcgagg tcgaccccga tgggtggatc
     2041 ttctacgagc ccacggcgtt catcgtcaac ccgggcggtc cttcggagct cggcccggtg
     2101 caagacaggc gaacgggcga gcggcgtctc gtctacgctc cacacttcta cgatccgacg
     2161 ctttttctaa acggcgacta ccagggccca gccgctatcg agaactggga agccaatcgt
     2221 ggcgcagaga tcgaggaacg acatccaggg ccgcttctga tcggcgagct cggcgccgga
     2281 ccgcctgcgt accatcacga cgtcttagcc atggccgacc gaatctgggc cggttggacg
     2341 cgctggtctt cggattcctt cttcgaggct ttcgaggatg ggagggagcc cgacgacatt
     2401 ctcgacatgg tccgggtcta cccgcaacgt atcgccggag acccaaccga cttcggctac
     2461 aacgttgcga agcgcgcgtt cagcctcaga ttcagcgaac gcgacggggt cgaagggcct
     2521 accgagattt acatccccga agcgcgcgtc tacccggatg gctggagg
//
LOCUS       DASRZF010000274         2972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1927826, whole genome shotgun sequence.
ACCESSION   DASRZF010000274 DASRZF010000000
VERSION     DASRZF010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1927826"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2251)
                     /gene="acnA"
                     /locus_tag="VFG22_12070"
     CDS             complement(<1..2251)
                     /gene="acnA"
                     /locus_tag="VFG22_12070"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HET6417026.1"
                     /translation="MSSKDSFQSRVTIHAGGRQVVIHRLDALTRAGFKGIDTLPYSIR
                     VLLENLLRYEDGKTVTRQDIEAVANWDPDAAPTTEIAFRPSRVLLQDFTGVPAVVDLA
                     AMRDQFAKMGGDPSRINPLQPADLVIDHSVQVDSYGLLRSFEDNVKREFERNQERYQF
                     LKWGQSAFRNMRVVPPGTGIVHQVNLEYLASVVFTRDANGQTIAYPDSLVGTDSHTTM
                     INGLGVLGWGVGGIEAEAAMLGQPISMLLPQVVGFKLTGKLREGATATDAVLTVTQML
                     RKKGVVGKFVEFHGPGMETLALPDRATIANMAPEYGATMGFFPVDNETLAYLRFSGRE
                     SAHVDIVERVLKEQGLFHTASDPEPRFTDTLDLDLGDVVPSIAGPKRPQDRIALTESK
                     RSWHDVLKGFEASADKSVEVTRGDETFRLTNGAVVIAAITSCTNTSNPSVMLAAGLVA
                     KKAVEKGLRTKPWVKTSLAPGSQVVTEYLEASGLMTDLEVLRFNLVGYGCTTCIGNSG
                     PLDPEIAEAVKANDLLVTSALSGNRNFEGRVNPLTRANYLASPPLVVAYALAGTMDID
                     FEADPIGQDTNGEDVFLRDIWPSQLEVRETLRTSVKKEQFVERYAVVLDGPPLWRVVS
                     FEEGDRYRWDPESTYVRLPPFFEGVDAGQPEPPEDIDGARVLCLLGDSVTTDHISPVG
                     AIALDGPAARYLQDHGVSPRDFNSYGSRRGNHEVMMRGTFANIRLRNLLAPGTEGGVT
                     RYLPDDAQMS"
     gene            2358..2432
                     /locus_tag="VFG22_12075"
     tRNA            2358..2432
                     /locus_tag="VFG22_12075"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2390..2392,aa:Cys,seq:gca)
     gene            complement(2468..>2972)
                     /locus_tag="VFG22_12080"
     CDS             complement(2468..>2972)
                     /locus_tag="VFG22_12080"
                     /inference="COORDINATES: protein motif:HMM:NF020348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heme-binding beta-barrel domain-containing
                     protein"
                     /protein_id="HET6417027.1"
                     /translation="DKGKDETPDDDRVSIEHNAFRERVVLEPIGRVDNHEQSLFGLRY
                     RKTAWRIGVEESFHEEVGYWLWDGAAKEVMLSFIVPRGVTVLAGGSVEPDAKSFKLSS
                     EVGSEVFGICSGPFLDREFKTLRFEIELTLNPDGSFSYDETTELQIKGQSKPFAHTDQ
                     NTLRRVG"
BASE COUNT          542 a          955 c          906 g          569 t
ORIGIN      
        1 tgctcatctg cgcatcgtcg ggcaggtagc gcgtcacacc gccctcggtg cccggtgcca
       61 ggagatttcg caggcggatg ttggcgaacg tcccccgcat catgacctcg tggttgccac
      121 ggcggctccc gtaactgttg aagtcacgtg gcgatacgcc gtggtcctgc aagtacctcg
      181 cggccgggcc gtcgagcgcg atggctccca ctggcgagat gtggtcggtc gtcactgaat
      241 cgccgagtaa gcaaagaaca cgtgcgccat cgatgtcctc gggcggctcc ggttgacccg
      301 cgtcgacacc ctcgaaaaac ggcggcaagc ggacatacgt cgactcgggg tcccaacggt
      361 agcgatctcc ctcctcgaag ctcaccacgc gccacagagg cggtccatcg aggacgaccg
      421 catagcgctc gacgaactgt tccttcttga ccgacgtccg gagcgtctcc ctgacctcca
      481 actgggatgg ccagatgtcg cggaggaaca catcttcacc gttggtatcc tgcccgattg
      541 ggtcggcctc gaagtcgatg tccatcgtcc cggcgagcgc gtaagcgacg accaggggcg
      601 gcgaagcgag atagttggcg cgggtcaacg gattgacgcg gccctcgaaa tttcggtttc
      661 cgctcagcgc agaggtgacg agtaagtcgt tcgctttcac cgcctcggcg atctcgggat
      721 ccaagggacc cgaattgccg atacaggtgg tgcacccgta gccgaccagg ttgaagcgca
      781 ggacctcgag gtcggtcatc aggcccgatg cctcgaggta ttcggtgacg acctgtgacc
      841 ccggagcgag gctggtcttc acccagggct tggttcggag tcccttctcg acggccttct
      901 tggccacgag cccggccgcc aacatcaccg aggggttgga agtgttggtg cagctcgtga
      961 tagccgcgat gacgaccgct ccgttcgtca agcggaaggt ctcatcgccg cgcgtgacct
     1021 ccacagactt gtcggccgag gcctcgaagc ccttgagcac atcgtgccaa gaacgcttcg
     1081 actcggtgag cgcaatgcgg tcctgcgggc gcttcggacc tgcgatcgat ggaacgacgt
     1141 cgccgagatc gagatcgagc gtgtcggtga acctcggttc ggggtcgctt gcagtgtgga
     1201 acagcccctg ctccttgagc acacgctcca cgatgtcgac gtgcgccgac tcccgcccgc
     1261 tgaagcgcag ataggccagg gtctcgttgt cgacgggaaa gaagcccatc gtggcaccgt
     1321 actcaggggc catgttcgcg atggtcgcgc ggtccggcag tgcgagggtc tccatgcccg
     1381 ggccgtggaa ctccacgaat ttcccaacga cgcccttctt ccggagcatt tgcgtcaccg
     1441 tcagaaccgc gtccgttgcc gttgcgcctt cgcgcagctt gccggtcagc ttgaatccga
     1501 cgacctgagg cagaagcatg ctgatcggct ggcccagcat cgccgcttcg gcctcgattc
     1561 cgccgacgcc ccaaccgaga acgcccagcc cgttgatcat ggtcgtatgc gagtcggtgc
     1621 ccactaggct gtccggatag gcgatagtct ggccattcgc atcgcgcgtg aagaccacgc
     1681 tagccaagta ctcgaggttc acctgatgca cgatgcccgt cccggggggc acaacgcgca
     1741 tgttccggaa ggcgctctgc ccccatttga gaaactggta gcgttcctgg ttccgttcga
     1801 actcccgctt gacgttgtct tcgaatgaac gcagcaggcc gtatgaatcc acttggaccg
     1861 aatggtcgat caccaagtcc gcgggctgaa gtggattgat gcgactcggg tcgccaccca
     1921 ttttcgcaaa ttggtcgcgc atcgccgcga ggtcgacgac cgcaggcaca cctgtgaagt
     1981 cctgaagcaa aactcgcgag ggcctgaagg cgatctccgt ggtcggcgcc gcgtccgggt
     2041 cccagttggc gaccgcctcg atatcctggc gggtcaccgt cttgccatcc tcgtagcgca
     2101 gcaggttctc gagcagaacc cggatcgagt acggaagcgt atcgatgccc ttgaagcccg
     2161 cccgcgtcag cgcatccagg cggtggatga cgacctgccg gcctccggcg tggatggtga
     2221 cacggctctg aaaggagtcc tttgatgaca tgtggaactg cctaccacgc aggttgtggc
     2281 gcgccacccc ggtgattggc taagctgaaa acgctgcgtg acaagcgctg tcagaagcgc
     2341 tagacaaggc agccttcggc gccgtagcca agtggtaagg cagaggtttg caaaatctcc
     2401 attcgtcagt tcgaatctga ccggcgcctc cagtttcgcc cccgggagtc aacgccctag
     2461 ggctgcccta gccgactctt cgaagcgtgt tctgatccgt gtgggcgaag ggcttgctct
     2521 gccctttgat ctgcaattcc gtggtctcgt catacgagaa cgacccgtcg gggttcaagg
     2581 tgagctcgat ctcgaagcgc agcgtcttga actcgcggtc gaggaacgga ccggagcaga
     2641 tgccaaacac ctcgcttcca acctccgagc tcaatttgaa cgacttcgca tcgggctcca
     2701 ccgaaccgcc cgcgagcacg gtgactccgc gcgggacgat gaagctcagc atcacctctt
     2761 tggccgcgcc gtcccaaagc cagtacccga cttcctcgtg gaacgactcc tcgacgccga
     2821 tcctccatgc cgtctttcgg tagcgaaggc caaagagcga ctgctcatga ttgtcgaccc
     2881 tgccgatcgg ctcgagcacc acgcgctccc taaacgcgtt gtgctcgatc gaaactcgat
     2941 cgtcatcggg tgtctcgtcc ttgcccttgt cg
//
LOCUS       DASRZF010000275        10007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2958468, whole genome shotgun sequence.
ACCESSION   DASRZF010000275 DASRZF010000000
VERSION     DASRZF010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 10007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10007
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2958468"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..673)
                     /locus_tag="VFG22_12085"
     CDS             complement(104..673)
                     /locus_tag="VFG22_12085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1 family glyoxalase III"
                     /protein_id="HET6417028.1"
                     /translation="MTEKPTALVLLAEGSEEMEAVITIDVLRRGGIEVTVAGIDGADP
                     IRCSRGVVITPDVALASVVGEFDVIVLPGGAEGAKRLAASSKVGDLLRAQESTGRYVA
                     AICAAPIALKAHGVFEHRQLTSHPSVKPELENWADYSEHSVVADLNLITSRGPGTAFP
                     FALRIVGTLTDAQRMVEVRAPMMFPVQVS"
     gene            complement(670..1275)
                     /locus_tag="VFG22_12090"
     CDS             complement(670..1275)
                     /locus_tag="VFG22_12090"
                     /inference="COORDINATES: protein motif:HMM:NF016184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur transferase domain-containing protein"
                     /protein_id="HET6417029.1"
                     /translation="MRYSAISLVSLALAGAIAGSTLRAYADGAPETPTSPRNIDGTPP
                     LVPIPNARVTTTGLLIGGQPSAEQLKAIQQAGYRTVITLRPESERGDEGEAAAVEQMG
                     MKFVRIPVAGAPGLTESNARALNAALVQRDALPAVVHCSIGQRVSALMGLRAFVVDRV
                     SPAAAMDLAKSLGLKELETALRDRIAQICKADPSRNCMGVQ"
     gene            complement(1279..2904)
                     /locus_tag="VFG22_12095"
     CDS             complement(1279..2904)
                     /locus_tag="VFG22_12095"
                     /inference="COORDINATES: protein motif:HMM:NF009472.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding dehydrogenase"
                     /protein_id="HET6417030.1"
                     /translation="MVGTSKYESEVVVIGAGLAGMVTALELLDAGKRVVMLDAASRDR
                     FGGLARWSFGGMFFVDSPEQRKNGIHDSVELAMRDWVRYGELDPNDVWPYRWAEAYVN
                     RCTEEVRDWLAERDVSFFRAVNWTERGYFVPGNSVPRFHIVWGTGEGIVLPLIPRLEA
                     HENAGRLAVHFDHRVEGLTQEGGQVVGCHGTNAVGSFEAKGDAVVIAAGGIAGSHDKV
                     REVWPRDQWGEPPEPMLNGSIPEADGRLLDRAEELGANITHLEKMWNYAAGVRHWAPL
                     FPNHGLSLVPAKSPLWMNYQGRRFVDPPLVGSYDTLLLIDRICREKKKYSWQVMNRKI
                     ADKEFAVSGAEFNQAVRDKKLLAFVVRLLKGNGEQVQEFIDHCPDFVTANTVTELAEK
                     MNALAGSQDVDAALLEREIADYDANIARGKKFQNDDQLRRIAHVRQYLGDRLRTCNMA
                     PIVDPDAMPLIAIRMQILTRKSLGGIQVDLEAKVLDKQGNAIPNLFAVGEACGFGGGG
                     MHGKRALEGTFLGGCVYSGRVAARAINSGQGAR"
     gene            3175..4104
                     /locus_tag="VFG22_12100"
     CDS             3175..4104
                     /locus_tag="VFG22_12100"
                     /inference="COORDINATES: protein motif:HMM:NF004846.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HET6417031.1"
                     /translation="MSHEWNLEQVPDLSGRVVIITGANSGIGYEAALALAKRKAEITL
                     ACRSEEKARAAVDRIRAEVPSARLKIIPLDLSDLASVRAFAERFREEQSQLHALVNNA
                     GVMALPRRLTVDGFEMQFGTNHLGHFALTGQLLDLLLATPASRVVNVSSIAHKFGKIR
                     FHDLQGEKRYSKWAAYGQSKLANLLFTHELDRRLKETGHETISVACHPGWASTNLQEA
                     GPKMEGSKLMEKLSAWGNAMLAQDAASGALPTVFATVEPLRGDEYIGPTRMMGWHGPP
                     EIIQPLEKARDPETAQRLWEASEKLTGVRFAFG"
     gene            4253..5143
                     /locus_tag="VFG22_12105"
     CDS             4253..5143
                     /locus_tag="VFG22_12105"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6417032.1"
                     /translation="MSGLRRERFGDFSILRRPGTGAAQVTVHFAHATGFNAETYRELF
                     AMLDPSIRLYAMDARGHGMSSAAADPRALRSWSPYRKDLERFVETIPPPVVLAGHSMG
                     ASVSLDLAAARPDLVQGLVLIDPVIPAPRQIPILALARPLGLTERMIPIARLAARRRM
                     EFASKEDAVANYVGKGAFRTWPREWIEAYVDGGTRPTEDGRVRLTCDRAWESQTFAKA
                     PVNPYHAIKKLRCPTTLIARVHDGPPFTEASREAFMKCKPDTRLVILEEASHFMTMEH
                     PEIAQAEIEAVVDGVRSELG"
     gene            complement(5129..7084)
                     /gene="nadE"
                     /locus_tag="VFG22_12110"
     CDS             complement(5129..7084)
                     /gene="nadE"
                     /locus_tag="VFG22_12110"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HET6417033.1"
                     /translation="MIPIKVATASLNQTPLDWAGNQQRIEDVLRQAKAEGVALLVLPE
                     LCTTGYGCEDAFLGPDVARRAWRMLTELLPQTEGMVVSVGLPVRRRGGLYNTACVVAD
                     GRIAGFAAKEHLAGEGIHYEPRWFKPWPNNQVVQLEQDGRSYPFGDVFFDFAGVRVGF
                     EICEDAWVAQRRGALDGYAMDIIANPSASHFAFGKHEVRERLVLEGSRSMAVSYLYAN
                     LLGNEAGRVIYDGGCYIAQNGKLLARSPRLTFDDSVLTTAVIDVEASQTEQVRTTSFQ
                     ADSTDPGSKRISVDFELPRARMERSVPLKPLWESSRHHKEEEFMRALTLALFDYLRKS
                     RSKGFVLSISGGADSAAAACLVALMVRRALDALGSERFAEKTGLPAHSSSRDWIGALL
                     CCAYQPTDNSGEVTRKAATAVAESIGATFYLLDVEHIVDAYERLISEAVGRPLTWEHD
                     DITLQNIQARVRGPSVWMLANMRGALLLSTSNRSEAAVGYATMDGDTCGGLSPIAGID
                     KAFLRRWLLWLETEGPEGMDPIPALRAVNAQTPTAELRPKDREQTDEGDLMPYPVLDA
                     IERLAIGEKHPPADCLEVLKGEFTEYDEQTLRDWVKRFFRLWSRNQWKRERYAPSFHV
                     DDKNLDPKTWCRFPILSGGFERELSEL"
     gene            7199..8863
                     /locus_tag="VFG22_12115"
     CDS             7199..8863
                     /locus_tag="VFG22_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6417034.1"
                     /translation="MDFRQASPTLGNQYDSDSLLREYLERTLPAELLERFEPAYRQLG
                     ELNGGELFRLSLADLDNEPVHTVWNPWGNRIDHVEVTDVWKRAQVISAEHGLVAAGYD
                     SELGSFARVHQHVLNYVAQASLDTYNCPLAMTDGAARTLLDSGNQALIDHALPRLLSR
                     DPATMWTSGQWMTERIGGSDVGQTETVARLDGDTWRLYGTKWFTSATTADMALTLARP
                     EGNPPGGRGLAMFYLELRDEHGRLQNLHINRLKDKFGTRKLPTAELTLDGVVAIPVAG
                     LENGVRNITPMLNITRTWNAVAAVLGMQRAVALTRDYADKREAFGAKLIDKPLHVDTM
                     AEMIAETQGAFLLAFRVVELLGRIEAGDASESEQLLSRLLTPIGKLTTGKQAVGVASE
                     ALESFGGAGYVNDTGLPRLLADAQVLPIWEGTTNVLSLDTLRALKQEGVWDVFIAEIQ
                     TRLRHATEPRLRGAAEHCEAAVQHAGRWLEATAERRDLLEAGARRFAMTLGRATELAL
                     LVDHAQWCLEQGKGDRALAAARRFARSRIDLVEDEGFMIESRLLVD"
     gene            complement(8865..9971)
                     /locus_tag="VFG22_12120"
     CDS             complement(8865..9971)
                     /locus_tag="VFG22_12120"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HET6417035.1"
                     /translation="MTRLLCLALTHAALLAACGDATESAESAGGSGGSGGVADTARPL
                     PWPAAEFPPLPKIAEDVPEARIRLGNLLFYDPILSVDKETACATCHSEFWGMGDALPV
                     AVGNGAGPLAGPGREGPNTLRRNSPALFNMAFRESLFWDGRSESLEEQAITPLLAEDE
                     LNRDPAEAVAELMGIAEYVNLFEEAFPEDPRITVDNLASALAAFQRTMVSDRSLYDAY
                     LRGHTATLNDELLEGMFLFAEMGCNDCHAPPLFESETFANRNVPSVDGIVDGGRGEIT
                     GLEEDLGAFRAPTLRNIFDSKPYFHNGAVKRLEDAVRHELEQSGMPFTDEDVRLIEAF
                     IAKALRDDSRMAERPRRVPSGLPTPLDGPIFPGR"
BASE COUNT         1819 a         3201 c         3132 g         1855 t
ORIGIN      
        1 ccggtttctt tgcgagctct gcagtataca agctcttgtc atacgtggcc cgagccaagg
       61 tttcggtaag gccacgcgtg acgcggccgc ccgagccgcc tacttaggag acctgcacgg
      121 gaaacatcat cggtgcgcgc acttcgacca tgcgctgtgc atcggtgagg gtgccgacaa
      181 tcctgagggc gaaggggaat gcggtgcccg ggccgcggct cgtgatcagg ttgagatcgg
      241 ccaccaccga gtgttcgcta tagtcggccc agttttcgag ctctggtttc acactcgggt
      301 gactggtgag ttgacggtgt tcaaagactc cgtgcgcctt cagcgcaatc ggtgcggcgc
      361 aaatcgccgc gacgtatcgg cccgtgctct cctgggcgcg tagcaggtcg ccgactttcg
      421 aagaggccgc cagccgtttc gcaccttcgg cgccgcccgg cagcacgatg acgtcgaact
      481 cgccgacgac gctagcaagg gccacatcgg gcgtgatgac gaccccgcgg ctgcatcgaa
      541 tcgggtcggc tccgtcaatg ccggccacgg tcacctcgat acctccacga cgcagcacgt
      601 cgatggtgat tacggcttcc atttcttcac tgccctcggc gagcaggacc agggcggttg
      661 gcttctcggt cattggactc ccatgcagtt tcgcgacggg tctgctttgc agatctgagc
      721 gatcctgtcg cgaagcgcgg tttcgagctc cttgagaccg aggctcttcg ccaagtccat
      781 cgctgcggcc gggctcactc ggtcgacgac gaacgccctg agccccatca aagcgctgac
      841 tcgttgcccg atgctgcaat gaacgaccgc agggagtgcg tcccgctgga cgagagctgc
      901 gttgagcgcg cgtgcattgc tctctgtgag acctggagcg cccgcaacgg gtatccgcac
      961 gaacttcatt cccatctgct ccaccgcggc agcttctcct tcatcgcccc gctcactctc
     1021 aggacgaagc gtgattaccg ttcgatatcc cgcttgctgg atggccttca gttgctcggc
     1081 agacggctgg cccccgatga gaaggccggt cgtcgtaacc cgggcatttg gaatcggcac
     1141 gagcggaggc gtcccgtcga tgttcctcgg cgaggtgggc gtttcgggcg cgccgtcggc
     1201 gtaggcacga agcgtcgacc cggcaattgc ccccgccaga gccagggaga ctaaagaaat
     1261 cgccgaatat cgcatgggtt acctcgcgcc ttggccgctg ttgatcgccc gcgcggcgac
     1321 acgacctgaa tacacgcagc ctcctagaaa tgttccttcg agtgcgcgct tcccgtgcat
     1381 tccgccgccg ccgaagccgc aggcctcgcc gaccgcgaat agattcggga tcgcgttgcc
     1441 ttgcttgtcc aggaccttcg cctcgagatc gacctgaatg ccgccgaggc tcttgcgtgt
     1501 caggatctgc attcggatcg cgatcagtgg catggcgtcc gggtcgacga tgggcgccat
     1561 gttacaggtg cgcaggcggt cgccgagata ctgccgaacg tgggcgatgc gacgcagctg
     1621 gtcgtcgttt tggaacttct tgccgcgtgc gatgttggcg tcgtaatccg cgatctcccg
     1681 ttcgaggagc gccgcgtcga catcctgact gccagcaagt gcgttcatct tctcggcgag
     1741 ctccgtcacg gtgttggccg tgacgaaatc ggggcaatga tcgatgaact cctgaacttg
     1801 ctcgccattc cctttcaaaa gacggacgac gaatgccaag agctttttgt ccctgacggc
     1861 ctgattgaac tcggctcccg aaaccgcgaa ctccttatct gcgatctttc ggttcatcac
     1921 ctgccaggag tacttcttct tctcgcgaca gattcggtcg atgagtaaca aagtgtcgta
     1981 ggaacctacc agcggcggat cgacgaagcg ccggccctgg tagttcatcc atagcggtga
     2041 cttggctgga accaggctca gtccatggtt tggaaagagc ggcgcccaat gccgcacacc
     2101 cgcggcgtag ttccacatct tttcgagatg ggtgatgttc gcaccgagct cctcggcgcg
     2161 atcgagcagc ctgccgtcgg cttcgggaat ggaaccgttg agcatgggct cgggcggctc
     2221 accccattgg tccctcggcc aaacttctct caccttgtca tggctgcccg ctattccgcc
     2281 tgccgcgatc accacggcgt cgcccttggc ttcgaaggag ccgacagcat tcgtgccgtg
     2341 acagcccacc acctggccgc cttcctgcgt gagcccctcc acccgatggt cgaagtgaac
     2401 cgcgagccgc ccggcgtttt catgagcttc cagcctggga atcaagggca gcacgatgcc
     2461 ttcacccgtg ccccagacga tgtggaagcg gggcacggag tttccaggaa cgaagtagcc
     2521 gcgttcggtc cagttgaccg cccggaagaa gctgacgtcg cgctcggcga gccagtcgcg
     2581 cacctcctcg gtgcaccggt tgacgtaggc ttccgcccag cggtacggcc acacgtcgtt
     2641 cggatcgagc tcgccatagc ggacccaatc gcgcatcgcg agctccaccg agtcgtggat
     2701 cccgttcttt cgctgctcgg gcgaatcgac gaaaaacatg ccgccgaagg accagcgtgc
     2761 gagcccgccg aagcgatcgc gtgacgcagc gtccagcatc acgacgcgct tgcctgcgtc
     2821 gagcagctcg agcgcggtga ccattccggc aaggccggcg ccgatgacga cgacctcgct
     2881 ttcgtactta ctcgtgccaa ccatagagag cagcgaggat agccccagta cccgtctcgg
     2941 cgccagcagt ctctgggact accgaaacta cggtgtcacg ctgtccgttg cagccggcaa
     3001 cgctaccgtc atagatcaga tccgcagcat ccgagtccgc atccgtggcc gtgcttcccc
     3061 gaacgccccc agggtgccga gtcgctgccc ggcgtaccac gaaaagatac ggcaaagcgg
     3121 cccaatttcc gctgcggcga gcggcgccgc gcgccagagg ctagcttacg ccccatgtcg
     3181 cacgaatgga atctcgaaca ggtccccgac ttgtccggtc gcgtggtcat catcacgggt
     3241 gccaacagcg ggatcgggta tgaagccgcg cttgcgctgg cgaaacgcaa agccgagatc
     3301 acactcgctt gccggagcga ggagaaggcg cgggccgcag tcgaccgaat acgcgccgaa
     3361 gttcccagcg ctcgattgaa gatcatcccg ctcgacctat cggacttggc ctccgtgcgc
     3421 gccttcgccg agaggttccg agaggaacag tcccagttgc acgcactcgt caacaacgcc
     3481 ggcgtcatgg cgcttcccag gcgtctcacg gtagacggat tcgagatgca gttcgggacc
     3541 aaccatctcg gccacttcgc gctcaccggt cagctactcg atctgctgct ggcgacgccc
     3601 gcttcgcgcg tcgtcaacgt gagcagcatt gcgcacaagt tcggcaagat tcgttttcac
     3661 gatcttcaag gggagaagcg ctacagcaag tgggcggcct atgggcagag caagctcgcc
     3721 aatctgctct tcacgcatga gctcgaccgg aggctcaagg aaacggggca cgaaacgatc
     3781 tccgtggcct gtcatccagg ttgggcgtcc accaacctgc aagaggccgg acccaagatg
     3841 gaaggctcca agctgatgga aaaactgagc gcatggggca acgcaatgct ggctcaagac
     3901 gcggcatcgg gggctctacc tacggtcttc gcgaccgtcg agccgctgcg cggtgacgag
     3961 tacatcggcc caactcgcat gatgggctgg cacggccctc ctgaaatcat ccagccgctc
     4021 gaaaaagccc gtgacccgga gaccgcacag cggctctggg aagcgtcgga aaagctgacc
     4081 ggcgtgaggt tcgcgtttgg ctagttgcgg ggcttgcgtg ggcggctccg gattgtaggg
     4141 tcccgctcgg agggcagatg cgcgagctca aagggaaagt cgcggtggtg acaggcgcct
     4201 gtcatggcac cattgtggct cgggtcttca agcggatttt cctcgaagca cagtgagcgg
     4261 tcttcgacga gaacgattcg gggatttttc gatcctccgg cgaccgggga ccggcgcagc
     4321 ccaagtcacc gtgcactttg cccacgcgac cggcttcaat gccgagacct accgtgagct
     4381 cttcgcgatg ctcgatccct cgattcgatt gtatgcgatg gacgcgcgcg gccatggaat
     4441 gtcctcggcc gctgccgacc ccagagctct gcgctcctgg agcccatatc gaaaagactt
     4501 ggagcggttc gtcgaaacga ttccgccgcc cgtggtgctc gcgggccatt ccatgggggc
     4561 cagcgtgagc ctcgatctcg ccgccgcgcg tccggacctc gtgcagggct tggtgctcat
     4621 cgatccggtc atccctgctc ctcgccagat tccgatcctc gcattggcca gacccctcgg
     4681 actgaccgag cggatgattc cgatagcccg gctggccgcg cggcggcgca tggaattcgc
     4741 ttccaaagaa gacgcggtcg caaattacgt cgggaagggc gccttccgaa cgtggccacg
     4801 cgaatggatt gaagcgtacg tcgacggtgg aacgcggccg actgaggatg gacgcgtgcg
     4861 tctgacttgc gatcgcgcgt gggaaagcca gacattcgcc aaagcgccgg tcaacccata
     4921 tcacgcgatc aagaagctcc ggtgcccgac cactttgatc gctcgtgtac acgatggccc
     4981 acccttcacc gaggcgtcgc gcgaggcgtt catgaagtgc aagcccgata cacgactcgt
     5041 gatcctcgag gaagcctcac acttcatgac catggagcac ccggagatcg cccaggctga
     5101 aatcgaggcg gtcgtagacg gagtccggtc agagctcgga tagctcgcgc tcgaagccac
     5161 cggacaaaat cgggaacctg caccaggtct tggggtcgag gttcttgtcg tcgacgtgga
     5221 acgatggcgc gtaccgctct cgcttccact ggttgcggga ccagagtcga aagaaacgct
     5281 tcacccagtc ccgcagagtt tgttcgtcgt actcggtgaa ctcgcccttc aagacttcta
     5341 ggcaatcagc aggtgggtgc ttctctccaa ttgcgagccg ttcgatcgca tcgagcaccg
     5401 ggtagggcat cagatccccc tcgtcggttt gctcgcggtc ctttgggcga agctctgcgg
     5461 tcggtgtctg tgcgttgacg gcacggagcg cggggatcgg gtccattccc tcggggcctt
     5521 cggtttcgag ccacaggagc cagcgacgaa ggaaggcctt gtcgataccg gcgatcgggc
     5581 tcagcccgcc gcaggtatcg ccgtccatcg tagcgtagcc gaccgcagcc tccgaccgat
     5641 tgctcgtcga cagcagcagg gcgccgcgca tgttggcgag catccacacg cttgggccgc
     5701 gcacgcgcgc ctggatgttc tgcagggtga tgtcatcgtg ctcccatgtg agcggccggc
     5761 cgaccgcttc ggagatcaat cgctcgtacg cgtccacgat gtgttccacg tcgagcaggt
     5821 aaaacgtcgc gccgatcgac tcggcgaccg cggtcgcagc cttgcgcgtg acctcgccgc
     5881 tgttgtcggt cggctggtag gcacagcaaa ggagcgcgcc gatccagtcg cgcgagctcg
     5941 aatgagcggg gagccctgtc ttttcggcga aacgctcgct ccccagggcg tcgagtgcac
     6001 gccggaccat gagcgcgacc aggcaagcag cggcagccga gtccgcccca ccgctgatgc
     6061 tgagaacgaa tcccttcgac cggctctttc gcaagtagtc gaagagggca agcgtgaggg
     6121 cccgcatgaa ctcctcttct ttgtggtgcc tgctcgattc ccagagcggc ttcaggggca
     6181 cgctgcgctc catccttgca cgcggtaact cgaagtccac gctgatgcgc ttggatcccg
     6241 gatcggtgct atcggcctgg aagctggtgg ttcgaacctg ctcggtctga gaggcctcga
     6301 cgtcgatgac cgccgtggtt agaaccgaat catcgaaggt aagtcgaggg gatcgcgcca
     6361 gcagcttgcc gttctgcgcg atgtagcagc cgccatcgta gatcacacgg cctgcctcgt
     6421 tgccgagcaa gttggcgtag agatagctca ccgccatcga tcgagatccc tcgagcacca
     6481 ggcgttcccg cacctcgtgc ttcccaaacg cgaagtgact cgcgctgggg ttcgcgatga
     6541 tgtccatcgc gtagccgtcg agtgcaccgc gccgttgcgc gacccaggcg tcctcgcaga
     6601 tttcgaaacc gacccgaaca cctgcgaaat cgaagaagac atccccgaac ggatagctcc
     6661 gtccgtcctg ctcgagctgc acgacttgat tgttgggcca tggcttgaac cagcgcggct
     6721 cgtagtgaat cccttcaccc gcgaggtgct ccttggccgc aaatcccgcg attcgaccgt
     6781 cggcaacgac gcaggccgtg ttgtagaggc caccgcgacg gcgaaccggc agtccgacgc
     6841 tcacgaccat gccctcggtt tggggcaaga gctcggtgag catgcgccag gctcgcctgg
     6901 cgacgtccgg accgaggaag gcgtcctcgc agccatatcc ggtggtgcaa agctcgggta
     6961 gcaccagcaa cgcgaccccc tcggccttgg cctggcgaag cacatcctcg atccgctgct
     7021 ggttgccagc ccagtcgaga ggcgtctggt tcaacgacgc ggtcgcaacc ttgatcggga
     7081 tcatgctgga tctttagcac cgctgcttgc ctgaggggtt caacggagat tgacggtcgt
     7141 tcgagggcgg cgtatgctga tttcacaagt cgtcactttc tgctattcgg agtaatgtat
     7201 ggacttccgt caagcatccc caacccttgg aaatcaatac gattcggact cgctccttcg
     7261 cgagtatctc gagcggacgc ttcccgccga gctgctcgaa cgattcgagc cggcctatcg
     7321 ccaacttgga gagctcaatg gcggtgagct ctttcggctc tccttggccg atctcgacaa
     7381 cgagcccgtg cacacggtct ggaatccctg gggcaatcgc atcgaccacg tcgaggtgac
     7441 cgacgtgtgg aagcgggctc aagtcatctc agccgaacat ggattggtgg cggccgggta
     7501 cgactccgag ctcgggtcgt tcgcccgcgt tcatcagcac gtgttgaact acgtcgcgca
     7561 ggcctccctc gatacataca actgcccgct ggcgatgacc gatggcgcag cacgaacctt
     7621 gctcgactcg ggcaaccaag cgctgatcga tcatgcgctt ccccgtctgc tgtcgcgcga
     7681 cccggcgacg atgtggacct ccggccagtg gatgaccgag cgcattggcg gctcggacgt
     7741 cgggcagacg gagacggtag cgcgcctcga cggagacacc tggcgtctct acggcaccaa
     7801 gtggttcact tcagccacga ccgcagacat ggcgcttaca ctggccaggc cggaagggaa
     7861 tcctcctggt ggccgcggct tggccatgtt ctacctagag ctccgtgacg agcacggccg
     7921 gctccagaac ctccacatca atcggctcaa agacaagttc gggacccgaa agctcccaac
     7981 cgcggagctc accttggatg gcgttgtggc tattcctgtc gcgggtctcg agaacggcgt
     8041 tcgtaacatc acgccgatgt tgaacatcac acgaacgtgg aatgcggtcg cagcggtgct
     8101 tggcatgcag cgcgcagtgg cgctcacccg cgactacgcc gacaagcgcg aggcgttcgg
     8161 cgccaagctc atcgacaagc cattgcacgt cgacacgatg gccgagatga tcgccgaaac
     8221 gcagggggca ttcttgctgg catttcgcgt ggtcgagctg ctcggccgaa tcgaagccgg
     8281 cgacgcaagc gaatccgagc agttgctgag ccgcttgctg actccgatcg gcaagctcac
     8341 cacaggcaag caggcagtgg gcgtggcttc cgaggctttg gaatcgtttg gcggcgcagg
     8401 ctacgtgaac gacacgggcc ttccaaggct gctcgccgac gcgcaagtgc tcccgatttg
     8461 ggaaggaacg accaacgtct tgtcgctcga cacgcttcga gcgctcaagc aagagggggt
     8521 ttgggacgtt ttcatcgctg agattcagac gcggcttcga cacgcaacgg agcctcgact
     8581 gcgtggggct gcggagcact gcgaggcggc ggtgcagcac gcggggcgct ggctcgaggc
     8641 cacggctgag cgaagggatc ttctcgaagc aggtgcgcgg cgtttcgcaa tgactcttgg
     8701 acgtgctacc gagctcgcac tgctcgtcga ccacgcgcag tggtgcctcg aacaagggaa
     8761 gggcgatcgc gccttggccg cggccagacg cttcgcacga agccgaatcg atctggtcga
     8821 ggacgaggga ttcatgatcg aaagccggct tctggtcgac tgagtcagcg ccccgggaag
     8881 atcggtccat cgagcggcgt gggcaggccg cttggcaccc ttcgcgggcg ttctgccatc
     8941 cggctgtcgt ctctgagcgc cttggcgatg aaggcctcga tgaggcgtac gtcctcgtcg
     9001 gtgaacggca tcccgctctg ctcgagctcg tggcgaacgg cgtcctcgag gcgcttgacc
     9061 gcgccgttgt ggaaataggg cttgctgtcg aaaatgttcc gcagcgtcgg agctcgaaac
     9121 gcgcccaggt cttcttcgag ccccgtgatc tcgccgcggc ctccatccac aatcccatcg
     9181 accgagggca cgttgcgatt cgcgaaagtc tccgactcga agagcggcgg cgcatggcag
     9241 tcgttgcagc ccatctcggc gaagagaaac atcccttcga gcagctcgtc gtttagcgtc
     9301 gccgtgtgcc ctcgaagata agcatcgtag agcgagcgat ccgagaccat cgttcgctgg
     9361 aacgccgcga gcgcagaggc gagattgtcg acggtgatcc ggggatcttc agggaaggcc
     9421 tcttcgaaca ggttgacgta ctcggcaatt cccatcagct ccgccacggc ctcggcgggg
     9481 tctctgttca gctcgtcttc cgccagcaat ggcgtgatcg cctgctcttc gagactttcc
     9541 gagcgcccgt cccagaacag gctctcacga aacgccatgt tgaagagcgc gggcgagttc
     9601 cggcgaagcg tgtttgggcc ctcccggccg ggtcccgcaa gcggacctgc cccgttgccg
     9661 acggcaaccg gcaacgcgtc gcccatgccc cagaactcgc tgtggcaggt cgcgcaggcc
     9721 gtctccttgt cgacggagag gatcgggtcg tagaagagga ggttgccgag gcgaatgcgg
     9781 gcctccggaa cgtcctcggc gattttgggt agcggtggga attccgctgc gggccaggga
     9841 agaggccgcg ctgtgtccgc tacgccaccc gatccgcccg acccaccagc ggactcggcg
     9901 gactcagtcg cgtcgccgca cgcggcgagg agcgccgcgt gcgtcagggc caggcaaagc
     9961 aagcgtgtca tcggtcactg ctgcgggcag ccggccgtta cccccac
//
LOCUS       DASRZF010000276         2315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_204025, whole genome shotgun sequence.
ACCESSION   DASRZF010000276 DASRZF010000000
VERSION     DASRZF010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204025"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..789)
                     /locus_tag="VFG22_12125"
     CDS             complement(175..789)
                     /locus_tag="VFG22_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417036.1"
                     /translation="MKSLTPILGLVALALLLSASPAVAELPSEEGAIVTVHTLESAPE
                     YPVLTRGRVLLIQAQRFVVTEEPLPAARVAEAPPEPQPDELERPNPAQPDALWVDGHW
                     AYGPTGFTWVAGHYVPPRVGHAFVPPRWASVDGQHLYFNGFFVPRGVYVRSFFNRYYY
                     SGTPNGQSRPSSGPYWPIGAPTRANSSLTSASVRDPYWPIGVRR"
     gene            905..2221
                     /locus_tag="VFG22_12130"
     CDS             905..2221
                     /locus_tag="VFG22_12130"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET6417037.1"
                     /translation="MSRQSTYPPPALAIEFELPAPIQTRPRPRVGVPQFTHARDCHSW
                     WVRAVNMLGGSLEPLGKPSADIYWERAQRLNPSAGDPTPETRAALVALTESLNQDAAL
                     NFIGKIAAWIDCTRMAATHLRVEQALREAPEVERTALPSPVFVLGLFRSGTTALHRLL
                     GEDPDNRTLPHWESFDPVHTPEGPEPRQRKLTRTLMLADLVSPSIKAIHPMDAYQTDE
                     CRGMFTNVFRTPQFNVQYRVGSYLNWLLEQDATVAYRHHRRQLQLVHHHRPYGKRFVL
                     KDPTHTFFVDAILEVFPNARFLFIHRDPAETLSSICSLHAYARSVFSSDVDAKAIGKE
                     ISESYMMRLLEPAVATVDRLPSGRVAHVRSPDLSRDPVGTIGEAYRTLGIELSDEARL
                     AMHGTMREKREGTGVRHIHSSEGFGIDPAEVHERFAGYCERFDLLA"
BASE COUNT          453 a          746 c          715 g          401 t
ORIGIN      
        1 atgtttcagt tcggcatcgg tctgaacgcg tacatctatg ggcacaacgc caagctcaac
       61 ctcgagtacc ggaaccgacc gatcttcggt accgacggca acgtcgaaag ccgagcgggc
      121 aactcgattg tgctgcagat gcagctattc atctaggatc gagaggagcc gggtctagcg
      181 ccgaacgccg atgggccagt acgggtcgcg gacgctcgca ctggtgagcg agctgttggc
      241 gcgagtcggt gctccgatgg gccagtacgg accgctgctc ggtcgagatt gtccgttggg
      301 ggtaccactg tagtagtagc ggttgaagaa gctccgaaca tagacgccgc gcggcacgaa
      361 gaagccgttg aagtatagat gctggccgtc gacggacgcc cagcgcggcg gaacgaaggc
      421 gtgtcccact ctcggtggaa cgtagtgacc ggccacccaa gtgaaccccg tcggtccgta
      481 ggcccaatgg ccgtcgaccc agagcgcgtc gggctgggct gggttgggcc tctcgagctc
      541 gtcgggctgg ggctctgggg gcgcctcggc aaccctggcg gccgggagcg gctcttcggt
      601 gacgacgaag cgctgggcct gaatcagtag aacccggccc cgcgtgagga ctgggtactc
      661 gggggcgctc tcgagggtat ggaccgtcac gatggcgccc tcctcgctcg gcagctccgc
      721 caccgcgggg ctggcgctca gcagaagcgc aagggcaaca agaccgagaa ttggggtcag
      781 gcttttcatc tcgactccgt ccggactcta cgcccagatc tgcccagggt cacgcaagcg
      841 tgggcccaaa aagccaaaaa ctacttacca agcggcgatc gtggtttctt acgccctgaa
      901 ccgcatgagc cgccagtcca cctacccacc cccggcgctc gcgatcgagt tcgagcttcc
      961 ggctccgatc cagacgcgac cgcgacccag ggttggggtt ccgcaattca cgcatgcacg
     1021 cgattgccat tcctggtggg ttcgcgcggt gaacatgctc ggcggttcgt tagagccgct
     1081 cggcaaaccc tcggcggaca tctactggga gcgcgcgcag cgcttgaacc cgagcgccgg
     1141 cgacccgaca ccggaaacca gggcggcgct ggtcgccttg acggagtcgc tcaaccagga
     1201 tgctgcgctg aatttcatcg gcaagatcgc ggcgtggatc gattgcacgc gcatggccgc
     1261 cacccatctc agagtcgagc aggctcttcg cgaagcgccg gaggtcgaac gaaccgccct
     1321 cccgagcccg gtattcgttc tcggcctatt ccgcagcgga accacggctc tccatcgctt
     1381 gctcggagaa gacccggaca accgtacgct ccctcactgg gaaagcttcg atccggtaca
     1441 cacgcccgaa ggacctgagc cgcggcaacg aaagctcacg cgaaccctga tgctcgccga
     1501 cctcgtgtca ccaagcatca aggcgattca cccaatggat gcgtaccaga ccgacgaatg
     1561 ccgcgggatg ttcacgaacg tttttcgaac gccgcagttc aacgtgcagt accgcgtcgg
     1621 aagttatctg aactggctgc tcgagcaaga cgcgaccgtg gcgtatcgcc accaccgtcg
     1681 ccaacttcaa ctggttcatc accatcgccc ctacgggaag cgattcgttc tcaaagaccc
     1741 gacgcatacc tttttcgtag atgccattct cgaggtgttt ccgaacgcgc ggttcctctt
     1801 cattcaccgc gacccggccg agactctgag ctcgatttgt agccttcatg cgtacgcacg
     1861 ctcggtgttc agcagcgacg tggacgcaaa agcaatcgga aaagaaatca gcgaaagcta
     1921 catgatgcga ttgctcgagc ctgccgtcgc gaccgtggac cgactccctt cggggcgcgt
     1981 cgcgcacgtc cgttcccccg acctgagccg cgatccggtg ggcacgatcg gcgaggcata
     2041 tcgcaccctg ggcatcgagc tcagcgacga ggcgcgactc gccatgcacg gcaccatgag
     2101 ggaaaagcgc gagggcaccg gcgttcggca catccacagt agtgaaggtt tcggcatcga
     2161 tcctgccgaa gtgcacgaac gcttcgcggg ctactgcgag cgcttcgacc tcctggccta
     2221 gtcgcttccg tccatcggcg cgccggcgtt ccaacaagcg tcatgagcca agagaaacgc
     2281 gtcgatgagc tctttgccgt cgcgcgagag tgtga
//
LOCUS       DASRZF010000277         2638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_204897, whole genome shotgun sequence.
ACCESSION   DASRZF010000277 DASRZF010000000
VERSION     DASRZF010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204897"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VFG22_12135"
     CDS             complement(<1..279)
                     /locus_tag="VFG22_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417038.1"
                     /translation="MNRVRQQFGLLAAVTIALLIGLVWIRVSSESRRELGAAERHRAD
                     GRILLAVEHYRRALRWSYPMSSSKEVAGLALESIALELEEEGDPQGALL"
     gene            complement(276..1211)
                     /locus_tag="VFG22_12140"
     CDS             complement(276..1211)
                     /locus_tag="VFG22_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417039.1"
                     /translation="MRVARIYAIALNTYREAVRDRVLFGVLVLAAGVLGLTLALGELS
                     LDQQMRVVTDLGLASISLFSVIVAIFLGSSLLYKEIERKTLYVILPKPIARFEFLLGK
                     YFGIVATCAVFIAIMGALQLLVTAIQAGASTVLVISVPLVLLVGLGIGFAFSKDRTLV
                     VPIWSLVALIASAWLCSTVHAELFRLIASLVLSLGEVLILTAVALLFSSFSTPFLTGA
                     FTFGVWLVGRAAHDMVAMETRVLSPSMKTVLRWLAEVVPNFNLFVPGRTTLVTKATEF
                     GAPLAYVATSMGYAILYAGVTLVVAALIFRRRDFL"
     gene            complement(1208..1861)
                     /locus_tag="VFG22_12145"
     CDS             complement(1208..1861)
                     /locus_tag="VFG22_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417040.1"
                     /translation="MPSYRRHVALTVWPLTALFVLGLLRMWPALRGARAFAAASPVTW
                     LSLSACTVLVFSFIAAYARGWSNFDANAGAGPYESMGCARLQKFAGWLGWAFVFAHLL
                     LQWFMTVRVGPVALSHYELLRGLISRPLALGFYVLGLGSLGLFLSQGLAASFRAWGFA
                     EKPETSRWLEVGCTLVTAVMMLMAVNVLSHFVTGRAYWSSSPAASGQTTADSERESP"
     gene            complement(1900..>2638)
                     /locus_tag="VFG22_12150"
     CDS             complement(1900..>2638)
                     /locus_tag="VFG22_12150"
                     /inference="COORDINATES: protein motif:HMM:NF013663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HET6417041.1"
                     /translation="DLGARAVLRYRPEPMPEEGSWDMIVTLGGDGTLLWASHLASSST
                     PMLAINSAPDTSVGYFCAGDGHNVDEVLGAALEGKLKSSRLARMRVDVGDETLSTRVL
                     NDALYCHESPAATSRYIIQHGGQQERQMSSGVWVGPAAGSTAAILSAGGKVLPTGSQK
                     IQYVIREPYRGVNNKYRMIKGMVAPGEALEITSRMTRGQIFLDGTQKVYPVVIGDRIR
                     MTLSDEPLTLLGLRGRDERTLRVSALK"
BASE COUNT          593 a          829 c          796 g          420 t
ORIGIN      
        1 caacaaggcc ccttgcggat cgccctcttc ttcgagctcc aaggcgattg actccagggc
       61 caagccggca acttccttgc tggagctcat ggggtaggac cagcggaggg cgcgacggta
      121 gtgttctacg gctaggagaa ttcgcccgtc ggcacggtga cgctctgcag cgccgagctc
      181 ccgccgcgat tcggaggaaa cgcgaatcca aaccaagccg atcagcaagg cgatcgtcac
      241 cgcggcaagc agcccgaatt gctgtcgcac gcgattcaca ggaagtctcg cctacgaaag
      301 atcaaggccg ccacgacgag tgtgacacct gcgtacagga tcgcgtagcc catcgaggtc
      361 gccacgtacg ccaggggcgc tccgaactcc gtcgccttcg taacgagtgt cgttcttcca
      421 gggacgaaga gattgaagtt tggcacgacc tcggccagcc aacgcagaac cgtcttcatg
      481 ctgggactga gcacgcgggt ttccatggcg accatgtcgt gcgcggccct gccgacgagc
      541 catacgccga acgtgaacgc acccgtgagg aacggcgtcg agaacgatga gaacaagagc
      601 gcgaccgcgg tcagaatgag aacctcgccg aggctcagaa ccaaggatgc gatcaaacgg
      661 aatagctctg cgtgcacggt cgagcagagc caggctgacg cgatgagcgc gacgagcgac
      721 cagatgggga cgaccagcgt gcgatccttg gaaaaagcga acccgatacc caggcccacg
      781 agtagaacga gaggcacgga aataacgaga acggtcgacg cgcccgcttg aatggcggtc
      841 accagcagct gaagcgcgcc catgatcgca atgaacaccg cgcaggttgc gacgatcccg
      901 aagtacttcc cgagcaggaa ttcgaagcgc gcgatcggct tcggaagaat gacgtagagg
      961 gtctttctct cgatctcttt gtagaggaga gaagagccta ggaatatcgc aacgatcacg
     1021 gagaaaagcg atatcgacgc gagtccgagg tcggtgacca ctcgcatctg ctggtcgagc
     1081 gagagctctc ccagtgcaag ggtcaatcca agaacgcctg ccgcgagcac caggactccg
     1141 aacagcactc gatctcgaac cgcttcgcga taggtgttga gcgcgatggc gtagatccgc
     1201 gcgaccctca tggcgactcc ctctccgaat ctgccgtcgt ctgacccgaa gcagcgggtg
     1261 agctcgacca gtatgcacgc ccggtgacga agtggctcag cacgttgacc gccatcagca
     1321 tcatgactgc ggtcacgagg gtacaaccga cttcgagcca gcgcgaggtt tcgggtttct
     1381 ccgcgaaacc ccatgcacga aaggatgctg ccaatccctg cgagaggaac aggccgagtg
     1441 acccgagccc gaggacgtag aacccgagcg cgagcggacg cgagataaga ccgcgcaaga
     1501 gctcgtagtg actgagagca actggaccaa cccggaccgt catgaaccat tgaaggagca
     1561 gatgggcaaa gacgaaggcc caaccgagcc atccggcaaa cttctggaga cgggcgcaac
     1621 ccatcgattc gtagggaccg gcacctgcat tggcgtcgaa attgctccag ccacgcgcat
     1681 acgcagcgat gaacgagaac accaagaccg tacaggcgct cagtgacagc caagtcacgg
     1741 gcgacgccgc tgcaaacgcg cgagcgccgc ggagggctgg ccacatccgc agcagcccca
     1801 ggacgaatag cgccgtgaga ggccaaaccg tcagcgcgac gtgtctacga tagcttggca
     1861 cggcctacta cctaccgccg cagcgtttgc ctggtcaatt tacttcaggg cggagaccct
     1921 cagagtgcgt tcgtcgcgac ctcgaaggcc gagaagagtc aggggctcgt cagacaaggt
     1981 catgcggatc cgatcgccaa tcacgacggg atacactttc tgcgtaccgt cgaggaagat
     2041 ctgtcccctc gtcatcctgc tcgtgatctc gagcgcctcg cctggcgcga ccatgccttt
     2101 gatcatccga tacttgttgt tgacgccgcg atacggctcc cgaatcacgt actggatctt
     2161 ctgcgaaccg gtcggaagca ccttgccgcc ggccgaaaga atcgctgcgg tggagccggc
     2221 agccgggccc acccagactc cgctcgacat ctggcgctct tgctggccgc cgtgctggat
     2281 gatgtaccgt gacgtcgcag ccgggctctc gtgacagtag agggcatcat tgagcacccg
     2341 tgtcgacagt gtttcgtcgc ctacgtcgac cctcatccgc gccagacggc tcgacttcaa
     2401 tttcccttcg agcgcagcgc caaggacttc gtccacgttg tgcccgtcac ctgcgcagaa
     2461 gtagccaacg ctcgtatccg gggcgctgtt gatcgccaac atcggcgtcg acgagctcgc
     2521 gagatgcgaa gcccagagaa gcgttccgtc ccctccaagc gtcacgatca tgtcccagga
     2581 accctcctcg ggcatgggct cgggccggta tcggagcact gcacgcgcac ccaagtca
//
LOCUS       DASRZF010000278         2808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4337716, whole genome shotgun sequence.
ACCESSION   DASRZF010000278 DASRZF010000000
VERSION     DASRZF010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4337716"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /gene="sufB"
                     /locus_tag="VFG22_12155"
     CDS             complement(<1..925)
                     /gene="sufB"
                     /locus_tag="VFG22_12155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HET6417042.1"
                     /translation="MSAATEQIDEILKKGYGAGFVTDIEQDYAPPGLSEDIVAFISQK
                     KAEPQWLLDWRLKAYRRWLEMKEPTWAKVSYPKIDYQDICYYAAPKTNENAPKSLDEV
                     DPELLATYEKLGIPLHEREVLAGVAVDAVFDSVSVATTFRDKLSEVGVIFMSFSEAVQ
                     KHPELIKKYLGSVVPVADNFFAALNSAVFIDGSFVYIPKGVRCPMELSTYFRINAAKT
                     GQFERTLIIAEEGSYVSYLEGCTAPMRDENQLHAAVVELVAHKDAQIKYSTVQNWYPG
                     DENGKGGIFNFVTKRGLCEGARSKISWTQVET"
     gene            complement(939..1430)
                     /locus_tag="VFG22_12160"
     CDS             complement(939..1430)
                     /locus_tag="VFG22_12160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly regulator"
                     /protein_id="HET6417043.1"
                     /translation="MLRISKMTDYAVMLATHLAASEGPHAARDLAVQTQIPEPTASKV
                     LKKLARAGVVISQRGAKGGYALARPPRQIGVNEVIEAIEGPIAVTECSDESTDSSCEY
                     EPSCGVRANWQRINFAVHTALSEITLADMALPGSTELVSIARSAEEAEQMRGAGLQSN
                     DVH"
     gene            1510..2064
                     /locus_tag="VFG22_12165"
     CDS             1510..2064
                     /locus_tag="VFG22_12165"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET6417044.1"
                     /translation="MAKTFKTFDAKLRAFIEQQKMFFVATAPLIQDGHINLSPKGLTG
                     TFAILDERTVAYLDLTGSGVETIAHLRENGRACIMFCAFEGPPRIVRVHGTGDVIEPE
                     HDEFESLRSHFDEYPGVRSIIRVRANRISDSCGYGVPLYDYAGERDQLQRWAERKGAD
                     GISEYQRENNTESLDGLPSLLGDH"
     gene            complement(2038..>2808)
                     /locus_tag="VFG22_12170"
     CDS             complement(2038..>2808)
                     /locus_tag="VFG22_12170"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET6417045.1"
                     /translation="AAQEAKDDATRLEAALEQELRKEQARIVGAGVECEVMVVRGRPW
                     RVIPELAEERGSFLITIGAYGASGQRSVDGKSKPELVLGSTAQRVVRAADRPVFVATG
                     DSPIPAGLRNTKWFVGTDFSESSLAAVAWAKRAAARVGGELHIANVVIPAGGEDKPDE
                     ERTWRQVLRDHSKIEAGMKLSEYVQEHAPGAEPHQIVSADYASHAMCDAAREVGANIL
                     VVGTHRQSVLGRFILGSTAARCLQIAPVPVLMVPEQAR"
BASE COUNT          525 a          877 c          832 g          574 t
ORIGIN      
        1 cggtctcgac ctgagtccag ctgatcttcg agcgtgcgcc ttcgcaaagg ccacgcttgg
       61 tgacgaagtt gaagatgcca cccttcccgt tctcgtcgcc agggtaccag ttctgcaccg
      121 tcgaatattt gatctgagcg tctttgtggg cgaccagctc gacgaccgcg gcatggagct
      181 ggttctcgtc gcgcatcggc gccgtgcagc cctcgaggta gctcacatag ctgccctctt
      241 cggcgatgat cagcgttctc tcgaactgac ccgtctttgc cgcgttgatt cggaagtaag
      301 tggaaagctc catcgggcat cgcacgccct tcgggatgta cacgaacgaa ccgtcgatga
      361 agaccgcgct gttgagcgct gcgaagaagt tgtccgcgac gggaacgacc gacccgagat
      421 atttctttat gagctctggg tgcttctgca ccgcttcgga aaacgacatg aagatgacgc
      481 cgacctcaga gagcttgtcc cggaacgtcg tggcgaccga aacactgtca aacacagcgt
      541 ccaccgctac gccggccagg acctcccgct cgtgcagagg aattccgagc ttttcgtagg
      601 ttgccagaag ctccgggtcg acctcgtcga ggctcttggg tgcgttctcg ttggtcttgg
      661 gcgccgcgta gtagcaaatg tcttgatagt cgatcttcgg atagctgact ttggcccagg
      721 tcggctcttt catctcgagc catcgccgat acgccttgag ccgccagtcc agaagccact
      781 gaggctccgc cttcttctga gagatgaagg cgacgatgtc ttcactcagc ccgggcgggg
      841 cgtagtcctg ctcgatgtcg gtgacgaaac cggcgccata gcccttcttg agaatctcgt
      901 cgatttgttc ggttgctgct gacatgagtc actccaactc agtggacgtc gttgctctgg
      961 agcccggcac cgcgcatctg ctctgcctct tcggccgagc gcgcgatgga cacgagctct
     1021 gtgctgccgg gcaacgccat gtccgcgaga gtaatttctg agagcgcggt gtgaaccgcg
     1081 aagttgatcc gctgccagtt ggctcgaaca ccacaactgg gttcgtattc gcaggacgaa
     1141 tcggtgcttt cgtcggagca ttcggtgacc gcgatgggcc cctcgatcgc ctcgatcact
     1201 tcattgaccc cgatctgacg agggggccga gcgagcgcgt agccgccctt tgcgccccgc
     1261 tgcgaaatca cgacgccggc gcgggcgagc ttcttgagaa ccttgctcgc ggtgggctcc
     1321 ggaatctgag tctgcaccgc caagtcgcgg gccgcatgag ggccttccga tgctgccagg
     1381 tgggtggcga gcatgacagc atagtccgtc atttttgaaa ttcgcagcat tttcggtgat
     1441 ttagataccg gactgtttcg gtactatatg gagatctaga gtactgccaa gtgggtgtca
     1501 aggttgccca tggccaagac cttcaagacg tttgacgcaa agctacgcgc cttcatcgag
     1561 cagcagaaga tgttcttcgt ggcgaccgct ccgctcattc aagacgggca catcaacctc
     1621 tcgccgaaag ggctcacggg cacctttgca atcctcgacg agcgaaccgt cgcctatctg
     1681 gatctcaccg ggagcggagt cgaaaccatc gcacatcttc gagagaatgg ccgtgcttgc
     1741 atcatgttct gtgccttcga aggcccccct cggatcgtgc gcgtgcatgg tacgggcgac
     1801 gtgatcgagc cggagcatga tgagttcgag agcttacgct cgcacttcga tgagtacccc
     1861 ggcgtccggt caatcatccg agttcgcgca aaccggatca gcgactcctg cggctacggc
     1921 gtgccgctat acgactacgc cggcgagcga gaccagcttc agcgttgggc ggagcgcaag
     1981 ggagcagacg gcatctcgga ataccaacgc gagaacaata ccgaaagcct cgacggccta
     2041 ccgagcttgc tcggggacca ttagcacggg cactggagcg atctgaaggc agcgagcagc
     2101 cgtgctgccg aggataaatc gccccagcac actctgcctg tgcgtgccaa cgaccaggat
     2161 gttcgcgccg acctctcgtg cggcgtcgca catggcatga gaggcgtagt cggccgaaac
     2221 aatctggtga ggctctgccc cgggtgcatg ttcttggacg tactccgaga gcttcattcc
     2281 agcttcgatc ttgctgtggt cccgcaagac ctgccgccag gtgcgctcct cgtcgggctt
     2341 gtcttcaccc ccggctggaa tgaccacgtt cgcgatgtgg agctcgccac cgacccgagc
     2401 cgccgcacgc ttcgcccagg cgaccgccgc caaagaagac tccgagaagt cggtgccgac
     2461 gaaccacttc gtgttccgaa gacccgccgg aatcggcgag tcgcccgtag cgacgaagac
     2521 gggacgatca gcggcccgaa cgacgcgctg agcagtcgaa ccgagaacca actcgggctt
     2581 gctcttgccg tcgaccgagc gctggccgct cgcaccgtac gctccgatgg tgatcaggaa
     2641 cgacccccgc tcttcggcaa gctctggaat cacccgccat ggtcgtcctc ggacgaccat
     2701 gacctcgcat tcgactccgg cacccacgat ccgcgcttgt tcttttcgca gctcttgttc
     2761 gagagcggcc tctagtcgcg tggcatcgtc cttggcttct tgcgcagc
//
LOCUS       DASRZF010000279         8408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_897928, whole genome shotgun sequence.
ACCESSION   DASRZF010000279 DASRZF010000000
VERSION     DASRZF010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 8408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8408
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897928"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VFG22_12175"
     CDS             <1..404
                     /locus_tag="VFG22_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417046.1"
                     /translation="PEPSPFEGDLPTQMFGLLWFLFLVPFVLVVLGTNPFQWFLYLFL
                     IPFVYVGGLTLLGPNASPFFVAAWLIGAPIVWSFFGRRRKKQKPGTRRRGSGWSAGSW
                     SSGGGGFSSSGGGGFSGGGGSFGGGGSSSSW"
     gene            complement(430..960)
                     /locus_tag="VFG22_12180"
     CDS             complement(430..960)
                     /locus_tag="VFG22_12180"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HET6417047.1"
                     /translation="MRTLVLDQGYQPHRIISWQRAVCMIFDGKVEVVEEYDEDVRSVS
                     ITIKMPAVVRLLRNIVGRKRAIKFSRINVAMRDDFKCQYCGMRHRLRGLTYDHVIPKS
                     QGGKTNWENIVMACYACNEKKGNHTPRQAGLRLRRPPTKPKWLPVIAFRVDPASSIPE
                     AWANWVYWHGRLDSDP"
     gene            1038..1424
                     /locus_tag="VFG22_12185"
     CDS             1038..1424
                     /locus_tag="VFG22_12185"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6417048.1"
                     /translation="MRGSELQEWTAIWRAMDPRTKFEIIEHASESYVAGILDGEVVAT
                     CLLVPEAAVVRMKRVAVASSSQGGGIGTHVLQFCEAHARSIGASTLTAHARETAVRFY
                     ANNGCATEGEYFEEAGIPHVAVRKHL"
     gene            complement(1691..1764)
                     /locus_tag="VFG22_12190"
     tRNA            complement(1691..1764)
                     /locus_tag="VFG22_12190"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1728..1730),aa:Arg,seq:cct)
     gene            1901..2992
                     /locus_tag="VFG22_12195"
     CDS             1901..2992
                     /locus_tag="VFG22_12195"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HET6417049.1"
                     /translation="MSNPPPTEDGPEERRLEISGRDSKRPSTLPDDLLFRSDEEAARR
                     LALKSLAAARSAERRLDDRFDRAALHEFRVAIRRLRSLLRAYRPQLETAIRAKDRKRL
                     RAIQRATGGGREADVALEWLTKQHADLAPEHLAGLNWLSALLLERRRKCAEDLDSDVR
                     AEFRRTAEKLEERLPLMRTEQNLLAEERHASFARTLANLTESHATELLVNLGEIASMD
                     QAAQLHQSRIAAKRLRYLLEPVRAYAAGAQGVVKRSKRLQEVLGDLNDIHVLMHEIER
                     SFETSMQHKATRVRDSLRRGDIERAKREASVSEWAGLVELYDRLEKERREIVSQIRDR
                     WLGGDLDDLVARARGLAHELRVMDSAPRL"
     gene            complement(2996..3526)
                     /locus_tag="VFG22_12200"
     CDS             complement(2996..3526)
                     /locus_tag="VFG22_12200"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002636.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HET6417050.1"
                     /translation="MSEERLDLLRRRIRDVPDFPKKGILFRDITPLLADVEAFRTSIQ
                     LMAEALDGESVHAVVGIESRGFMFGAALADKLGVGFVPVRKPGKLPAATYRAEYDLEY
                     GSDAVEIHQDALSPGDRVVVVDDLIATGGTAQATAELVRQCGAEVAAFVFLIHLVGLE
                     GIEKLAPARVITILDY"
     gene            complement(3523..4287)
                     /gene="surE"
                     /locus_tag="VFG22_12205"
     CDS             complement(3523..4287)
                     /gene="surE"
                     /locus_tag="VFG22_12205"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HET6417051.1"
                     /translation="MSDRPLLLVSNDDGVDAPGIIALRTALKSFADVYTVAPQTEQSA
                     KSHSITLHHPLRFHEVEEHVWAVDGTPADCVYVAFFLKDMLPRMPDLVVSGINHGPNL
                     GNDVHYSGTVAAAREGALRGVRSIAFSNSSSDMEGAAQYAKRFCKQLLSTTEPPGQAV
                     LLNVNFPSVPPKGIRATRLGFRLYSDEVEVRDDPRGRHYLWIGGPGGATSEPVEGADT
                     EALEEGFISITPLSIEATQPNHLGVAAYLAGPTEDP"
     gene            complement(4378..4451)
                     /locus_tag="VFG22_12210"
     tRNA            complement(4378..4451)
                     /locus_tag="VFG22_12210"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4415..4417),aa:Pro,seq:ggg)
     gene            complement(4562..5017)
                     /locus_tag="VFG22_12215"
     CDS             complement(4562..5017)
                     /locus_tag="VFG22_12215"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET6417052.1"
                     /translation="MTRRTQLPDKLFFKIGEVADIVGVKPHALRYWESEFPALRPKKT
                     RGAHRQYSRRDVELAMLIRQLLHDEGFTIPGARKRIRELGRHQRTSPPEPRAQREVAL
                     RAELLGLRQQLAELLEGLSVEESAPAEARPVQVTVHKVVPVTVSRGPEV"
     gene            complement(5026..5346)
                     /locus_tag="VFG22_12220"
     CDS             complement(5026..5346)
                     /locus_tag="VFG22_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HET6417053.1"
                     /translation="MTKAEIIDAVYEKVGGFSKKEAAEVVEAVFDTMKDVLAVGEKIK
                     ISGFGNFVVRAKKERIGRNPQTGAPIPISSRRVLTFKPSQVLKTVLNPNRTDEDGAGS
                     QESL"
     gene            complement(5470..7950)
                     /gene="pheT"
                     /locus_tag="VFG22_12225"
     CDS             complement(5470..7950)
                     /gene="pheT"
                     /locus_tag="VFG22_12225"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HET6417054.1"
                     /translation="MLVSFRWLQELCPVDADVAEVARRISLAGLEVEALEEKGKNLSD
                     VVIAEVRAKTPHPKKDKLTLVTVFDGDNEHQVVCGAPNVPEPGGRVLFARSGAVLPNG
                     MEIAPRKVAGVDSSGMICSEAELEIGADAAGIFVLEPDSQALPGTAVADALDLHDVIL
                     DIGLTPNRPDCLGHIGIAREIGVLFGADTSLRRAASPQRTFAAGSAFESGEPTSELTP
                     LWDDETKPVPTPELPSISVEIVDGDRCPRYGAALVSGVKVAPSPFWLRYRLHNLGLRA
                     LSNVVDATNLILLEWGHPIHGFDLARLGGSKVVVRKAREGETMATLDDVERSFTADDL
                     LICDADGPVAVAGVMGGLGTEIRPDTEQVLIECAYFDPRSIRRTSRRLGLHTDSSHRF
                     ERGVDPEAIPRVLASAASLIAELGGGAAATTAIDRIATPFEPKSIRFRPARAARLLGT
                     PVSKEESLRILGSLGCRVVEKGDASITVQAPSWRPDLGREVDLIEEVARVRGYEEIPT
                     AVPRVHPSEAGTSSLLKFVDRLRDAAVASGLYEAVNYGFVSLEDLRKARVSTDAVPLA
                     NPLSEERSVMRTSLLPGLTAAATRAQRHGASEVRLFELARTFAPIDEVLPREDTVLAI
                     ALAGERSGWIGGAEAFDLYDGKGVIEAVVEAVFGQVPELVAGEAPSFLHPKRSALVSL
                     AGRSIGFVGEIHPDVGDELGLIGRVVYAELDAEGLHALSEELGPTQAKGLPKFPAVAR
                     DIAMLVRDEFSAGEIADALRAASDGLAESVSLFDLYRGDQIPAGHRSLGFRVTYRDPE
                     GTLTDKRVDKVHESLARLASDRFEATIR"
     gene            complement(7959..>8408)
                     /locus_tag="VFG22_12230"
     CDS             complement(7959..>8408)
                     /locus_tag="VFG22_12230"
                     /inference="COORDINATES: protein motif:HMM:NF013568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HET6417055.1"
                     /translation="DDDATHSPMFFQLEGFLVDEDVTFAELKGVLTLFLRRLFDEHIA
                     VRFRASYFPFVEPGGEVDMSCVFCRAWEGDRARTAECRVCKGTGWMEILGCGMIHPVV
                     FENVGYDPEKYTGFAFGMGLDRIAMLKYGIPNIKLLYENDVRFLSQF"
BASE COUNT         1575 a         2573 c         2759 g         1501 t
ORIGIN      
        1 ccccagagcc gagtccgttt gaaggcgact tgccgactca gatgtttggc ctgctctggt
       61 tcttgttcct cgtgccgttt gtcctggtgg tactcggcac gaacccgttc cagtggttcc
      121 tctatttgtt cttgatcccg ttcgtgtacg tcggtgggct gacgctgctc gggccgaacg
      181 cgagcccgtt cttcgtcgcg gcttggctca tcggggcccc gatcgtctgg tcgttcttcg
      241 gacggcggcg aaagaagcag aagccgggca cgcggaggcg cggctcggga tggtctgctg
      301 gctcttggtc ctcgggcggc gggggctttt cgagctcggg cggaggcggg ttctccggtg
      361 gcggaggaag cttcggcggc gggggctcgt cgagcagctg gtgaaccgcc gctgcgcagc
      421 tgccgggttt tagggatcgc tatcgagacg accgtgccag tacacccagt tcgcccaggc
      481 ctcggggatc gagctcgccg ggtcgacccg aaacgcaatc acggggagcc acttcggctt
      541 ggtcggtggt cgcctcaggc gaaggcctgc ttgcctgggc gtgtggttgc ccttcttctc
      601 gttgcaggcg tagcaggcca tcacgatgtt ttcccagttc gttttgccgc cttgcgactt
      661 gggaatcacg tggtcgtagg tgagaccgcg gagacgatgg cgcatgccgc agtactggca
      721 tttgaagtcg tcacgcatcg cgacgttgat ccgcgagaac ttgatggcgc gcttccggcc
      781 cacgatgttc cggagcaggc gcacgaccgc gggcatcttg atggtgatgc tgaccgagcg
      841 aacgtcctcg tcgtactcct cgacgacctc caccttgcca tcgaaaatca tgcagacggc
      901 gcgctgccaa ctgatgatcc gatggggctg atagccctgg tctaagacga gggtgcgcat
      961 ttcttacgag cagtcgtata gcgcgaacgg gcgaagaagt gaaccggcct cggtgacggt
     1021 ccggcaaaaa atgcgatctg cgcggctcgg agctacagga atggacggcg atctggagag
     1081 cgatggaccc ccgcacgaaa ttcgagatca tcgaacatgc gagcgagagc tacgtcgcgg
     1141 ggattctcga tggggaagtc gtggcaacct gccttctggt cccggaagct gccgtggttc
     1201 ggatgaagcg cgtcgccgtc gcatcgagtt cgcagggcgg gggaatcggc acgcacgtgc
     1261 ttcaattctg tgaagcccac gcgcgttcga tcggggcctc cacgctgacc gcccatgcga
     1321 gagagacggc ggttcgcttc tacgcgaaca acggctgcgc cacggaggga gagtatttcg
     1381 aggaggcggg aataccccac gtcgcggtgc ggaagcacct gtagcggcga ccgatctggc
     1441 gacgcaaagc cgtcgccggg cgtgaatatc gagcaacggt ccgacgcagg gcccgcgcat
     1501 cagggtgggg aaaatccgaa cgacgttgaa cagaaagcgt gaatgtcacc gcacctgatg
     1561 gccgaattga ccgataaggg cggccctcga tttgggagca gcggcttgat aagaaaagcc
     1621 cggtcgaagc caagattgga acacgcggca tacagaaagg ccccgagacg atttgtgatc
     1681 tcggggccgg cgtccccggg aggatttgaa ccaccgacct tcggtttagg aaaccactgc
     1741 tctatccgac tgagctacga ggacttaggt ggccgcgtat agcacgcagc tggtgtggcg
     1801 ggcgagcggt tgacgtcgga gccgcttctg tactaggtgt gatcggctca ggagtcgcat
     1861 catcactgga gaaagacgat atcccgcgcc gctcatcgcg gtgtcgaatc cgcctccgac
     1921 ggaggacggg cccgaagaac ggcggctgga aatcagcggc cgggattcga agcgtccaag
     1981 cacgcttcct gacgacctgc tctttcggag cgacgaagaa gctgcccggc gcctggcgtt
     2041 gaagagtctc gcggcggcgc ggagcgcgga acgcaggctg gacgaccgtt tcgaccgagc
     2101 cgcgctgcac gaatttcgag ttgcgattcg gcggctccgt agcctcttgc gcgcgtatcg
     2161 cccgcagctc gagacggcga tccgtgcaaa agaccgcaag cgactgcgag cgatccagag
     2221 agccaccgga ggcggtcgag aggccgacgt cgcgctcgag tggttgacca agcagcatgc
     2281 ggaccttgcc ccggagcact tggcgggcct gaactggctc tccgcgctcc tgctcgaacg
     2341 gcggcgaaaa tgcgcggagg atctcgatag cgacgttcgg gccgagttcc gacgcacggc
     2401 cgagaagctc gaagaacggc tgcctctgat gcgaaccgag cagaacctgc tcgctgaaga
     2461 gcgccatgcg agctttgctc ggacgctggc gaacttgacc gagtcacacg ccacggagct
     2521 actggtgaac ctcggtgaga tcgcaagcat ggaccaggcc gcacagcttc accaatcgcg
     2581 catcgccgca aagcggctcc ggtatctcct cgagccggtg cgcgcctacg cagccggagc
     2641 gcaaggggtc gtcaagcgga gcaagcgcct gcaagaggtg cttggggatc tgaacgacat
     2701 ccacgtgctg atgcacgaga tcgaacgatc cttcgagact tcgatgcagc acaaggccac
     2761 tcgggtccgc gactcgctcc gccgaggaga catcgagcgc gcgaaacgcg aagcgtcggt
     2821 gagtgagtgg gccggtctcg tcgagctcta cgaccgactc gagaaagagc gccgcgagat
     2881 cgtctcccaa atccgcgacc gttggctcgg cggcgacctc gacgacctgg tcgcgagggc
     2941 gcgcgggctt gcgcacgagc tgcgcgtcat ggattcggca ccgcgcctct gacgtttagt
     3001 agtcgaggat cgtgatgacg cgtgcgggcg cgagcttctc gatgccttcg aggccgacca
     3061 ggtggatcaa gaacacgaag gccgcgactt cggcgccgca ctggcggacg agctccgccg
     3121 tcgcctgggc cgtaccgcct gtggcgatca aatcgtcgac tacgacgact cgatctccgg
     3181 ggctaagcgc gtcctggtga atctcgacgg catcgcttcc gtattcgaga tcgtattcgg
     3241 ctcgataggt ggcggcgggc agcttgccgg gctttcgaac cggaacgaaa cccacgccga
     3301 gcttgtctgc caaggccgcg ccaaacatga agccgcgtga ctcgatgccg accacggcgt
     3361 ggaccgactc tccatcgagc gcctctgcca tcagttgaat cgaggtccgg aaggcctcga
     3421 cgtcggcgag cagcggcgtg atgtccctga agaggatgcc cttcttcggg aagtcgggga
     3481 cgtcgcggat tcgtcgacgc aatagatcga gccgctcctc gctcatggat cctcggtggg
     3541 gccggccagg tacgctgcaa cgccgaggtg gttgggctga gtggcctcga tgctgagggg
     3601 cgtgatcgag atgaagcctt cttcgagcgc ctcggtgtcc gcgccctcga cgggctcgct
     3661 tgtcgccccc cctggaccgc cgatccagag gtagtgacgc ccgcgcggat cgtcgcgcac
     3721 ttcgacctcg tccgagtaca agcgaaagcc aagccgcgtc gctcgaatgc ccttgggagg
     3781 aaccgaaggg aaattgacgt tcaaaaggac ggcctgcccc ggtggctcgg tcgtcgagag
     3841 caactgcttg cagaagcgct tggcgtattg cgcagcacct tccatgtcgg acgaggagtt
     3901 cgagaacgcg atcgagcgga cgcctcgaag tgcgccttcg cgagcggccg ccacggtgcc
     3961 cgagtagtgc acgtcgttgc cgaggttggg accgtgattg attcccgaca cgactagatc
     4021 gggcattcgc gggagcatgt ctttcaagaa gaacgcgaca tacacacagt cggctggggt
     4081 tccatcgacg gcccaaacgt gctcttccac ttcgtggaaa cggagcggat ggtgcagtgt
     4141 aatcgaatgg ctcttggcgc tctgctcggt ctgcggggcg acggtgtaaa cgtccgcgaa
     4201 cgacttcagc gcggttcgca gtgcgatgat ccctggcgcg tcgacgccat cgtcattgga
     4261 aaccaataga aggggtcggt ccgacatgcg atgaacgacc tatcgcggat gctccgaggg
     4321 ggcaaagaga gggcaaaggg agaaccgggg ggctttcgcc ctccggtccg cccatagtcg
     4381 gggcgactgg atttgaacca gcgaccccct cacccccagt aaggtgcgct accaagctgc
     4441 gctacgcccc gtccaccctc tattcgaggg cacgcgactc taacccagga cgaccagaac
     4501 gcaaatgaaa gaggagtcga aaaaccggtg aaatgcaccc gaaagcggac gaaaaagggg
     4561 cctagacctc ggggccgcgg ctaactgtga ccgggaccac tttgtgaacg gtcacctgca
     4621 cgggtcgagc ttctgcgggc gcgctctcct cgaccgaaag accctcgaga agctccgcga
     4681 gctgctgtct gagcccgagc agctcggcac gcaacgctac ctctcgctgc gcacgcggtt
     4741 cgggcggact cgtgcgttgg tgacggccta gctcacggat gcgctttcgg gcgccgggga
     4801 tcgtgaagcc ctcgtcgtgg aggagttggc gaatgagcat cgcgagctcg acgtcgcgtc
     4861 ggctgtactg acgatgcgcg ccgcgcgtct tcttcggacg caaagccgga aactcggatt
     4921 cccagtagcg gagcgcgtgc ggcttgacgc cgacgatatc cgcgacctca ccgatcttga
     4981 agaacagttt gtcgggcagt tgggtgcgcc tcgtcatcgt tcagctcaga gactttcttg
     5041 cgagcccgcg ccgtcttcgt cggtacggtt cgggttgagc actgttttga ggacctgact
     5101 gggcttgaag gtcagcacgc gccgcgagct gatcggaatg ggggcgcccg tctgcgggtt
     5161 tcggccgatg cgctccttct tggcgcgcac cacgaagttt ccgaaaccgg aaatttttat
     5221 cttctctcct acggccaaca cgtccttcat cgtgtcgaac acagcctcga cgacttcggc
     5281 cgcttccttc ttcgagaagc ccccaacctt ctcgtagacg gcatcgatga tttccgcttt
     5341 ggtcattcgg tctattcctc cagaggcccg gtatgtcgga gagaaccaca aatcggtttg
     5401 aatctcaagg gtttggcggg tcgtggcgcc gataaaaagt gcatattctc acacatcact
     5461 aggtatctct cagcggatgg ttgcctcgaa gcgatcgctt gcaagcctag cgagcgactc
     5521 gtggaccttg tctacgcgct tgtcggtcaa ggtgccttcg gggtcgcggt aggtcacgcg
     5581 gaagccgagg ctgcgatggc ccgcggggat ctgatcgccg cggtagaggt cgaataggct
     5641 cacggactcc gccaggccat cggatgccgc tcgaagcgcg tcggcgatct cgccggcgct
     5701 gaactcgtct cttaccagca tggcgatgtc gcgggcgacc gccggaaact ttggcagccc
     5761 ctttgcctgg gtggggccga gctcctcgga cagagcatgg agaccctccg catcgagctc
     5821 cgcgtagacg acccggccga tcagaccgag ctcgtcaccc acatcgggat gaatctcgcc
     5881 gacgaagccg atggaacgcc cggctagaga taccagtgcc gatcgcttcg gatgtaggaa
     5941 gctcggcgct tccccagcga cgagctcggg cacctgccca aacacagcct cgacgacggc
     6001 ctcgatgaca cccttcccgt cgtacaagtc gaaggcctca gcgccaccga tccagccgga
     6061 gcgttcaccg gccaaggcga ttgcaagaac ggtgtcttct ctcgggagca cctcatcgat
     6121 gggggcgaag gttcgagcca actcgaacaa gcggacctcg cttgcaccgt ggcgctgggc
     6181 gcgtgtcgca gccgcggtga gcccgggcag caaggaggtt cgcatgaccg agcgctcttc
     6241 ggagagcggg ttcgcgagcg ggaccgcatc ggttgagacc cgggccttgc gaaggtcttc
     6301 gagcgacacg aagccgtaat tgacggcctc atagaggcct gatgcgacgg ccgcgtcgcg
     6361 cagccgatcg acgaatttga gaaggctcga ggtgcccgcc tccgatggat gaacgcgtgg
     6421 caccgcggtg gggatctctt cgtagcctcg gacgcgcgca acctcttcga tgagatctac
     6481 ctcgcggccg aggtccggcc tccagctcgg ggcctgtacc gtaatcgacg catcgccctt
     6541 ctcgacgacg cggcaaccga gggatcccaa aatgcgaagg ctctcctctt tcgacacggg
     6601 cgtaccgaga agtctcgccg ctcgtgcggg acgaaagcgg atcgactttg gttcgaaagg
     6661 cgttgcgata cggtcgatgg ccgtcgttgc agcggcaccc ccaccgagct ctgcaatgag
     6721 cgatgcggcg ctcgcgagga ctcggggaat ggcctcagga tcgactccgc gttcgaagcg
     6781 atgactcgaa tcggtgtgca ggccgagccg tcgcgaggtg cgacgaatcg aacgagggtc
     6841 gaagtaagcg cactcgatga gcacctgctc ggtgtccgga cgaatctccg tgccgagccc
     6901 ccccatgaca ccggcgactg cgaccggacc atcggcatcg cagatcagga gatcgtcggc
     6961 cgtgaagctg cgctcgacgt cgtcgagggt cgccatcgtt tcaccctcgc gtgccttgcg
     7021 gacgactacc ttggaccctc caagccgcgc aagatcgaaa ccgtgtatcg gatgacccca
     7081 ttcgagcaag atcagattgg tggcgtcgac cacgttcgag agcgcgcgaa gccccagatt
     7141 gtgcaagcgg tagcgaagcc agaacggtga tggcgcaact ttgacaccgg acacgagcgc
     7201 agcgccgtat cgcggacatc ggtcgccgtc gacgatctcg acggaaatcg aggggagctc
     7261 aggcgtcggc accggcttgg tctcatcgtc ccagagcggg gtgagctcgc tcgtcggctc
     7321 gccggactcg aaagcgctgc ccgccgcgaa tgtcctctgc gggctcgcag ctctccgtag
     7381 gctggtgtcg gcgccgaaga gaaccccgat ttcccgggcg attccgatgt gtccgaggca
     7441 atcagggcga ttgggggtga ggccgatgtc caagatcacg tcgtgcaggt cgagcgcatc
     7501 ggcgacggcg gttccaggaa gcgcctggct gtccggctcg agcacgaaga tccccgcggc
     7561 gtccgctccg atctcgagct cggcctcgct gcagatcatg ccgctcgaat ccacgcccgc
     7621 gactttccgc ggagcgatct ccatgccgtt cggcaagacg gccccggagc gggcgaacaa
     7681 gacgcggcca cctggctcgg gcacgttggg tgctccgcag acgacctgat gctcgttgtc
     7741 gccatcgaag acggtgacca gggtgagctt gtccttcttt gggtgcgggg tcttggcgcg
     7801 aacctcggcg atgacgacgt ccgagaggtt cttgcccttt tcttcgagcg cctcgacttc
     7861 gaggccggcg agcgagatgc gtcgcgcaac ctcggcgacg tccgcgtcga cggggcagag
     7921 ctcttgcaac cagcgaaatg aaaccagcat cgctcttcct aaaactgcga caagaaccga
     7981 acgtcgttct cgtagaggag cttgatgttg ggaatgccat acttcagcat cgcgatgcgg
     8041 tcgagcccca tgccgaacgc aaagccggtg tacttctcgg gatcgtatcc gacgttctcg
     8101 aagacgaccg gatgaatcat gccgcagcct aggatctcca tccatccggt gcccttgcag
     8161 acgcggcact cggctgtgcg cgcgcggtca ccttcccagg cgcgacagaa gacgcagctc
     8221 atgtcgactt ccccgcccgg ctccacgaac gggaagtaac tcgcgcgaaa gcgaactgcg
     8281 atgtgctcgt cgaagaggcg gcgcaggaag agcgtgagta cgcccttgag ctcggcaaac
     8341 gtgacatctt cgtcgaccag gaagccctcg agctgaaaga acatcggcga atgggtggca
     8401 tcgtcgtc
//
LOCUS       DASRZF010000280         3140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4675127, whole genome shotgun sequence.
ACCESSION   DASRZF010000280 DASRZF010000000
VERSION     DASRZF010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4675127"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VFG22_12235"
     CDS             <1..257
                     /locus_tag="VFG22_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417056.1"
                     /translation="FKAKSEGIRLVKSAFDAENVEMPEPIYRVYVRETDPSPSQEVVH
                     QAESVRREGDTAADERLDIQVEAERAEGAPDLLDENAPRE"
     gene            complement(265..1959)
                     /locus_tag="VFG22_12240"
     CDS             complement(265..1959)
                     /locus_tag="VFG22_12240"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6417057.1"
                     /translation="MILLNPKKHERLYPDERSREVMLKTIEFFERKGKRRIKEDYHAR
                     VWYADFLDFVKEEKIFATMCTPADHGAPNSRWDTWRICEFAEILGFYGLQYWYTYQVT
                     VLGLGPIWMSENESIKQRAARALEQGGIFAFGLSEKEHGADVYSTDMTLTPRGDGKYS
                     ASGSKYYIGNGNKAAIVSTFGKLADSGEYVFFGVDSQHERYNLIKNVCASQNYVSEFA
                     LDDYPIEENDIIAKGDHAWNSALNTVNIGKYNLGWASIGMCTHALYEAIHHASNRRLY
                     NMYVTDFPHVKQLFTDAYARLTAMKLFALRAADYMRVASANDRRYLLYNPMVKMKVTT
                     QGEDVMNLMWDVIAAKGFEKDMFFEQAAVDIRALPKLEGTVHVNIALIVKFMANYFFA
                     PADYPEVGQQSQARNDDFLFDQGPTKGLGKIRFHDYQIAYSSRDTPNLRVFREQIEAY
                     KAMLFAAPPDEAQRKDIDFLLAGGEIFALVVYGQLILENAKIYDVSDDLVDQIFDFMV
                     RDFSRHALNLHSKPTSSDTQMAACMKIIRKPVFDGKRYQRVWEEVHALRDEYEMNP"
     gene            complement(2004..2405)
                     /locus_tag="VFG22_12245"
     CDS             complement(2004..2405)
                     /locus_tag="VFG22_12245"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HET6417058.1"
                     /translation="MDGYPVELEIPVAWGDLDAFGHVNNTVYFRWFESARMAFFERIG
                     IGPNRPEKVGPIMATTSCDYLVPVEYPATVIVGARVQRIGNTSFVMENEATLEGTPVA
                     RGTTVIVLIDYRTGEKVPVPDDMRAAIAALS"
     gene            2593..>3140
                     /locus_tag="VFG22_12250"
     CDS             2593..>3140
                     /locus_tag="VFG22_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417059.1"
                     /translation="MKHALTILLLVLFSLPLTAGAEDVCPEFVNMVRVISEDGKTSTE
                     LGMEKAVAKQKKKGIYRGYDQSVRLEAPNAKVDVKPSQSFAFKPFNAEIHPAQQIKLY
                     PFNPKKDYRELAVGGTNMWGGSKNKKSADNSIDLTFSKISDGCYKVTPEVTLPKGEYA
                     FSLGSVGLGSSADVKGTNAGWGS"
BASE COUNT          640 a          931 c          913 g          656 t
ORIGIN      
        1 tcttcaaggc gaagagcgaa ggcattcgat tggtgaagtc tgccttcgat gcggagaacg
       61 tcgaaatgcc cgagccgatc taccgcgtgt atgttcggga gacggacccg agcccatcgc
      121 aagaggtcgt gcaccaggcc gagagtgttc gccgagaagg cgacaccgca gcagacgagc
      181 ggctcgacat ccaggtcgag gcagagcgcg ccgaaggagc gcccgatctc ctcgacgaaa
      241 acgcgccgag agaatgagct cagctcaggg attcatctcg tactcgtcac ggagtgcgtg
      301 aacctcttcc cagacgcgtt gatagcgttt tccatcgaag accggcttgc ggattatctt
      361 catgcaagcc gccatctgtg tgtcgctgct ggtcggcttc gagtgcagat tcaacgcatg
      421 gcgcgagaag tcgcgcacca tgaagtcgaa gatctgatcg acgaggtcgt cggagacgtc
      481 gtagatcttt gcgttctcga gaatcagctg gccatagacc accagcgcga agatctcgcc
      541 tcctgccagc aagaagtcga tgtcctttcg ctgcgcttcg tcgggaggcg ccgcgaagag
      601 catcgctttg taggcctcga tctgctcgcg gaagacacgg aggttggggg tgtcacggct
      661 cgagtatgcg atctggtagt cgtggaaacg gatcttcccg agccctttgg tcggaccttg
      721 atcgaaaagg aaatcgtcgt tgcgcgcctg actttgctgg ccgacctctg gatagtccgc
      781 aggggcgaag aagtagtttg ccatgaattt cacgatgagc gcgatgttca cgtgaaccgt
      841 gccctcgagc tttggtaggg ctcgaatatc caccgcggcc tgctcgaaga acatgtcctt
      901 ctcgaatccc tttgctgcga tgacgtccca catgaggttc atcacgtctt cgccttgtgt
      961 ggtcaccttc atcttcacca tcgggttgta gagcaggtaa cgccggtcgt tcgccgaagc
     1021 gactcgcatg tagtccgccg cgcgaagcgc aaatagcttc atcgcggtga gacgcgcata
     1081 tgcatccgtg aatagctgct tcacatgagg aaaatcggtc acgtacatgt tgtagaggcg
     1141 ccgattcgaa gcgtgatgaa tggcttcgta aagcgcatgc gtgcacatgc cgatcgaggc
     1201 ccatccaagg ttgtacttcc cgatgttcac cgtgttgagc gcgctattcc atgcatggtc
     1261 gcccttggcg atgatgtcgt tctcctcgat cggataatcg tcgagcgcaa actccgagac
     1321 gtagttctgg ctcgcgcaga cgttcttgat caagttgtag cgctcgtgct gggaatcgac
     1381 cccgaagaag acgtattcgc cggagtccgc aagcttgccg aaagtcgaga cgattgccgc
     1441 cttgttcccg ttgccaatgt agtacttgct gccgctggcg ctgtacttgc cgtccccgcg
     1501 cggggtgagg gtcatgtcgg tagagtacac atcggcgccg tgctccttct cagagagccc
     1561 aaacgcaaat attccaccct gctcgagcgc gcgcgcggcc cgctgcttga tcgactcgtt
     1621 ctcactcatc cagatcggcc cgaggccgag cacggtaacc tggtaggtgt accagtactg
     1681 caatccgtag aagccgagaa tctccgcgaa ctcgcaaatc cgccaagtgt cccatcgcga
     1741 attcggcgcg ccgtggtctg ccggcgtgca catcgtcgcg aagatcttct cctctttgac
     1801 gaaatcgagg aagtccgcgt accagacccg tgcgtggtag tcctctttga tacgccgctt
     1861 gcccttgcgc tcgaagaact cgatggtctt gagcatcact tcacgtgagc gctcgtccgg
     1921 atagaggcgc tcgtgctttt ttggattgag gaggatcata gtcgctttct gataccacga
     1981 cgcccgcact cggtggaggc tttctagctc agtgccgcaa ttgccgcacg catatcgtcg
     2041 ggcacgggca ccttctcgcc agtccggtag tcgatcagca cgatgaccgt agtgccacgg
     2101 gccaccgggg ttccctccag ggtcgcttcg ttctccatga cgaaactcgt attgccgatg
     2161 cgctgcacgc gggcgcccac gatgacggtg gccgggtact cgaccggcac gagatagtca
     2221 cagctcgtcg tcgccatgat cgggccaacc ttctcgggtc ggttcgggcc gatcccgatg
     2281 cgctcgaaga acgccatgcg cgccgactcg aaccatcgga agtagaccgt gttgttcacg
     2341 tggccgaacg catccaggtc gccccaggcg actgggattt cgagctcgac ggggtagcca
     2401 tccatcggtg aaacctatct ctcccgccga gccttcgcca gccggtcatg cgggttgcat
     2461 ccggccgtgg tcggagtgtt ggtgagcgat ttctgtgccc gcgacgatgt ctttccagaa
     2521 actacgtgtt tgtagtgact cgcgacttca tcttgtgttt taatggcagc gtcgtagcga
     2581 aggaatgatt tcatgaaaca cgccctgacc atcttgctcc tcgtcctctt ctccctccct
     2641 ctcaccgcag gcgcggaaga cgtctgcccc gagttcgtca acatggtccg ggtgatcagc
     2701 gaagacggga agacgagcac ggagcttgga atggagaaag ccgtcgcgaa gcagaagaag
     2761 aagggcatct acaggggcta tgaccaaagc gtccgcctcg aggccccaaa cgcaaaggtc
     2821 gacgtcaagc cgtcacagtc tttcgccttc aaacccttca acgccgagat ccaccctgct
     2881 cagcagatca agctctatcc tttcaacccg aagaaggact acagagagct cgccgtaggc
     2941 gggacgaaca tgtggggagg cagcaagaac aagaagtccg ccgacaactc gatcgacctc
     3001 acgttctcga agatcagcga cggctgctac aaagtgaccc cagaggtcac acttcccaaa
     3061 ggcgaatacg ccttcagcct cggctcggtc ggcctcggct catcggcaga cgtcaaaggc
     3121 accaacgccg gctggggctc
//
LOCUS       DASRZF010000281         9511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2274496, whole genome shotgun sequence.
ACCESSION   DASRZF010000281 DASRZF010000000
VERSION     DASRZF010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 9511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9511
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2274496"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            439..1005
                     /locus_tag="VFG22_12255"
     CDS             439..1005
                     /locus_tag="VFG22_12255"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HET6417060.1"
                     /translation="MSRSKGKEPEGRQPSKADREALERAFADVKPLGGASRKRVMPAP
                     EAPAIRGRATGRAAGTPAEALLVEREGNGIVFGKRRSTHSSILDALEDPKLEVEAECD
                     LHGQTAREAEREVHRFVRESQRDGKRWVLVIVGKGLHSPGGKGTLKTSIVDALSKRSP
                     ARFVLAFRTAPRHLGGGGALVARLVDRL"
     gene            complement(1035..1433)
                     /locus_tag="VFG22_12260"
     CDS             complement(1035..1433)
                     /locus_tag="VFG22_12260"
                     /inference="COORDINATES: protein motif:HMM:NF017007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HET6417061.1"
                     /translation="MAQGDTHNLGMGYLLWIFGFMGAHRFYYGRPVSGTIWFLTLGLL
                     GIGWLIDLILMPSLDRSAELRYDSGPVSHNVSWILLTFLGIFGIHRFYMGKYLTGLIY
                     FFTGGLFFVGILYDYWTLNEQVSEANAARA"
     gene            complement(1526..1687)
                     /gene="rpmG"
                     /locus_tag="VFG22_12265"
     CDS             complement(1526..1687)
                     /gene="rpmG"
                     /locus_tag="VFG22_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET6417062.1"
                     /translation="MRDLIKLSCETCGKDNYVTDKNKRTMPEKFHIKKFCPKCRSHQP
                     HKEGKISKG"
     gene            1806..3746
                     /locus_tag="VFG22_12270"
     CDS             1806..3746
                     /locus_tag="VFG22_12270"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HET6417063.1"
                     /translation="MFDTKRAAGTPAQYVARESETIGEMFLRRVALSGDQPAFYFKDG
                     GDWQAVGWNQFFEQSAAVAAYLMGLGLGIQDKVCMVGSTRPEWCCCDVGGQLAGAVTL
                     GAYPTLTPKQLAYILDHADAKIAFVEGIEEVNKILARRDELTKLEKVVVWNTAGVESL
                     MKAHKWLVPYDRVLQTPFDRKAIEARTSEIDPNSTAIIVYTSGTTGPPKGAMISQKNV
                     MTIMRDQDEAFSIVEEDVSMSFLPMAHVAERVLAFYGRINTGMVTYFASSIAKVLEEI
                     AETKPTVFGSVPRIFEKAYAKIMSTVEQAPPTKQRIFRWAEKVGREAVRRWQSGEPIS
                     LGLKLKYALADRLVFSKLRDIFGGRVRYFITGAAPIAPEILEFFWAAGFPIFEVYGMT
                     ESTVITHANVPGAVKLGSVGRALSVVEDRIAADGEILVRSNLVFQGYYKNPEATAETV
                     IDDWLHTGDVGKKDDDGFVYILDRKKHIIITAGGKNLTPANIENELKASDSLISQAHA
                     HGDKRKFLVALVTLGAPELVDWARNEGSIDAATANKHTKDLSENPLARSAELQALLRQ
                     LAHDPEVQARVVASVKRANESLSRVETIKKIFILDREFSVEEDELTPTLKLKRKNVET
                     KFKDTFDRLYDDEAFGLTVLSA"
     gene            3759..4451
                     /locus_tag="VFG22_12275"
     CDS             3759..4451
                     /locus_tag="VFG22_12275"
                     /inference="COORDINATES: protein motif:HMM:NF025729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HET6417064.1"
                     /translation="MRFALTCLTVMLLASPALAFEGLLEASLSSEQGVAAEVSARYSK
                     AGDVRMDVKSKDADGQPVLMSTIMPAKGSSYYSIQHAQQIIIEVPYSKLAETSQQVTG
                     SGDNANLEIEKLGKATVSGVPTEHVRVIDKDNEAVIDLWITQKYPADLWTRAFRGRNL
                     GLEMSDDERTKAMKKYGVKPGFSMKMRVEQAGGVPVVFLVKKIERGPVPAEVFSLPEG
                     YKRVQSQGGSQP"
     gene            4522..5550
                     /locus_tag="VFG22_12280"
     CDS             4522..5550
                     /locus_tag="VFG22_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417065.1"
                     /translation="MTKQVLLRALTLLSVLLVLNGCKSDTAKDAKSSDPVLVLDAGQE
                     PRVPLRYKIARGTTTTATMDFGIASLATSSSGGAALTVTPGVRLHIVSGPSLEGKRGA
                     TRFDVRIVKAEAIVPKGIDPAVAQDLNKSASVLNNVGSWVEVDDRGIIQRTQLNDAAQ
                     RSDVPVRLLVMIINARTSLARVLLPAEPVGVGARWEARKDLTLYGFKVNQVDTYTLTE
                     QVGDELKLNVQIQQTAAPQTITFEEEGVELSVESFKMNAAGEVIANLNALEANAVASG
                     DSEGLINVKTVDGTEEIEIDRAFQVRMTVTYEVAQTAAAAAEASEAAQAAAEAEKEAA
                     KKLEKAQE"
     gene            complement(5541..7745)
                     /locus_tag="VFG22_12285"
     CDS             complement(5541..7745)
                     /locus_tag="VFG22_12285"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HET6417066.1"
                     /translation="MTDGEAPQDLGERIRQIATSIAGEVDKRLLTGEESGSIFDAAAR
                     SLGSTMERWGRNPGLRRFLGGVQRDVLTSKGERVELSASLGVSAKLGTTARFYVDDEV
                     VGEVPIDPSGEVRTMILAPEPGIHRVGVKICNDRGQVVSDLIGHRLLQVASGRPVVLV
                     HAELFLPGAPKEPTPRRAPMDALRTLIDEGFELVYFDIHEKNRDSLIHDELLKQRLPP
                     GAILVYSAEEEELRSMGVDFVDMFASTAIRRLRAKGVPVTTILTDRDEDSERIQQERV
                     SVLTPESVRDRALSGAFVTELKQATELLRKRDESNPLDWRLDQTTESRLVCGNAFHAE
                     LDNGEARRQLFAALEQASSTVHIQLYMVRPSDFTERLIVLLIRSARAGVKVRFMVDAL
                     YSDEEVFGRINPLIQSLREEDNIEVLALNPIDAGKQVAVSRFKQRDHRKLTIIDGKRA
                     FVSGRNAADEYFLGFDEVPVHDNTRHERIPWLDAHVEVAGPLVRQVQETFMRTWHRHG
                     GAEIPVDSSVMPELEEAGSVCGRLIVHRGLAETNGLAMYEAMLDLAEEHVYIVNDFPI
                     IPALERAIYRLLARDVSVKLLTGNATARRDDGTFFPAPLHRTLFEYMVKAKLEPLLLA
                     GVRIYEFVPPPSPNVVSRGGRIRPYVHAKIVSVDGLVTSIGSANLDATASFWESEANV
                     VVQDPNFARGIEAKLQEMIDGSVELDPESEYWKRERTQRAVVATLWPGSLYS"
     gene            complement(7765..8289)
                     /gene="hpt"
                     /locus_tag="VFG22_12290"
     CDS             complement(7765..8289)
                     /gene="hpt"
                     /locus_tag="VFG22_12290"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HET6417067.1"
                     /translation="MQIEPLIDADTIAARVADLGRQISEDYRGKELVVVPVLKGSYVF
                     AADLVRQIDMEVAIDFLGVRSYGGGKESSGVVQITTDLSHSIEGKHVVLVEDIVDTGL
                     TIAYLHQNLATRQPASVRVAALLHKPARTLVPVQIDYLGFTVDDVFVVGYGLDYAQKF
                     RNLPYLAVIADDDT"
     gene            complement(8300..>9511)
                     /locus_tag="VFG22_12295"
     CDS             complement(8300..>9511)
                     /locus_tag="VFG22_12295"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HET6417068.1"
                     /translation="SFSSMPPFVDSRTSVDDSVAFAATVAAAEVRSSSGMAMGMDFAP
                     LADSLQPEGSEPDIFKEMSQPPAPLGPSNVFGSSPQLGYFSGFPAGPDETVAVPSVPP
                     KAVLASVEPRRGRSLGLVAVIIAVTVFSAVVAFLAFERYGDSLLRDLLEGTEPVSASA
                     PSRPSTRAAMNAEKPAPEADLAAEEQPVGAAESAELVEGEAVGAVEAVEAVEQDGAPQ
                     EENVEGDDGKLAVEPPNPRDDGPKIAPTPRPKARAERRAAPKPEPVAEPVAPERDSVS
                     DADQKVLAEFRSNANAAPAKIEVEKASTAQSENPPLDGDGVRATVTENKPRLQRCYER
                     AIRGLQSPPAVRLDVTVTVASSGRVKNVSAAGSGPGGLAECIEASVRRWRFPASSEGG
                     PAKFPIVFSPN"
BASE COUNT         1903 a         2974 c         2963 g         1671 t
ORIGIN      
        1 cggatgcagt gcgcgtccgc aaggcgatcc tccaatgcaa cctgcgccca aagcgcgaag
       61 cgcccaaaaa agcctaggct gggcgcccgg atggggctcg attacgaaag acgccagtcg
      121 cacggccggt gtatgcccca gcgtcatggc gagcgccgat ggctggttgt gcaaatccgg
      181 gaaatccgcc gcagactccc gggcacgggg gagagtccaa gcacttcggc tcgcctgaca
      241 caccactccg tagccgccga ctgacgtcca tcgatgccag ctgttgcaac ggctgtgcca
      301 cgcgcgagcc cacataaatc cagggaaagt cgtggcaccg ctggcgccgg aggcgaagaa
      361 tcttcgcgtc tcagccgata gccgcatcgc ttgcgcagat tgcccctcgc cccgatgcca
      421 ggtcatcatg gatcggtcgt gtcgagatca aagggaaagg aaccagaggg tcgccagcca
      481 tccaaggccg atcgcgaggc gctcgagaga gcattcgccg acgtgaagcc gctcggcgga
      541 gcctcacgaa agcgcgtgat gcccgctccc gaagctccgg cgatccgcgg ccgagcgacg
      601 ggccgagcgg cggggacccc ggccgaagcg ctcctcgtcg agcgtgaagg caacggcatc
      661 gtcttcggca agcgacgttc cacgcactcc agcattctcg atgccctcga agacccgaag
      721 ctcgaagtcg aagccgagtg cgatctgcat ggacaaaccg ctcgagaagc ggagcgcgag
      781 gtccatcgct tcgttcggga gtcgcagcgc gatggcaaac gatgggtcct cgtcatcgtc
      841 ggcaaaggcc ttcacagccc cggcggcaaa ggcacgctca agacgagcat cgtcgatgcg
      901 ctgagcaaac gttcgcctgc gcgtttcgtt ttggcctttc gaacggcccc ccggcatctc
      961 gggggcggag gggcgctcgt cgctcgcctc gtcgaccgtc tctagacgaa ggccccgaga
     1021 gctccgcaag agaactaggc gcgggccgcg ttggcctcgc tgacctgctc gttgagcgtc
     1081 cagtagtcgt acaaaatgcc gacgaagaac aatccgcccg tgaagaagta aatgagcccc
     1141 gtcagatact tccccatgta gaagcggtga atcccgaaga tcccgaggaa cgtgagcaga
     1201 atccagctca cgttgtggct cacgggcccc gagtcgtagc gaagctcggc cgaacgatcg
     1261 agcgagggca tgaggatcag gtcgatcaac cagccgatgc cgagcagccc cagggtgagg
     1321 aaccaaatcg tcccgctgac cgggcgcccg taatagaaac ggtgtgcgcc catgaacccg
     1381 aagatccaga gcagatagcc catcccgaga ttgtgcgtgt ccccctgcgc catgcggggt
     1441 ttctacgatg cgccaggggc tcgcggcaag tgggcggctt gcggcccatt cgcctcaaac
     1501 cgtccgagaa gaggcgatcc ccttctcagc ccttgctgat cttgccttct ttgtgcggct
     1561 gatggctgcg gcacttcggg cagaacttct tgatgtggaa tttctccggc atcgtgcgtt
     1621 tgttcttgtc cgtcacgtag ttgtctttgc cgcaggtttc gcagctgagc ttgatgaggt
     1681 cgcgcattag attcccttgc ctttttgggc tggaaggggg cgcagtgtag cggggcttcg
     1741 ggccgggtca aatcccgcct cgccggccta gatccgggac ccagatccgg tgtaaacccc
     1801 cgaagatgtt cgatacgaag cgcgcggccg gcactccggc tcaatacgtc gctcgcgaaa
     1861 gcgagaccat tggcgagatg ttcctcaggc gcgtggcgct gagcggagac cagcccgcgt
     1921 tctacttcaa ggacggcggc gactggcaag cggtcggctg gaaccagttc ttcgagcagt
     1981 ccgccgcggt cgccgcttac ctgatgggcc tcggcctcgg catccaagac aaggtctgca
     2041 tggtcggcag cacccgcccc gagtggtgct gctgcgatgt cggcggacag ctcgccggcg
     2101 ccgtgacctt gggcgcctac ccgaccctca caccaaagca gctcgcctac atcctggacc
     2161 acgccgacgc gaagatcgcg ttcgtcgagg ggatcgaaga ggtcaacaag atcctcgcac
     2221 ggcgcgacga gctcacgaag ctcgagaagg tcgtcgtctg gaataccgct ggcgtcgagt
     2281 cgctgatgaa agcgcacaaa tggctcgtcc cttacgatcg ggtgcttcaa acgcctttcg
     2341 accggaaggc gatcgaagcg cggacctccg aaatcgaccc aaacagcacg gcgatcatcg
     2401 tctacacgag cggcacgacg ggcccaccca aaggcgcgat gatctcgcag aagaacgtca
     2461 tgacgatcat gcgcgaccag gacgaggcgt tttcgatcgt cgaggaagac gtgagcatga
     2521 gctttctgcc gatggcccat gttgcggagc gcgtgctcgc gttctacggg cgcatcaaca
     2581 ccgggatggt cacctacttc gcttcgagca tcgccaaggt gctcgaagaa atcgccgaaa
     2641 ccaagccgac cgtctttggc agcgtgcccc gcatcttcga gaaggcgtac gcgaagatca
     2701 tgagcacggt cgagcaggca cctcccacga agcagcgaat ctttcgctgg gcggagaagg
     2761 tcggccgcga ggcggtccgc cgctggcagt caggcgagcc catttccttg ggcttgaagc
     2821 tgaagtacgc cctggccgac cggctcgtgt tctcgaagct acgagatatt ttcggcgggc
     2881 gggtccgcta tttcatcacc ggcgccgcac cgatcgcccc ggagatcctg gagttcttct
     2941 gggcggcggg attcccgatt ttcgaagttt acgggatgac cgagtcgacc gtgatcacgc
     3001 acgccaacgt gcccggtgcg gtgaagctcg gctcggtggg tcgcgcactg agcgtggtcg
     3061 aggaccgcat cgccgctgac ggcgagatcc tcgttcgaag caacctggtc tttcaggggt
     3121 actacaagaa cccggaagcc acggccgaga ccgtcatcga tgactggctc cataccgggg
     3181 acgtcggcaa gaaggacgac gacggattcg tctacatcct cgaccgaaag aagcacatca
     3241 tcatcaccgc gggaggaaag aacctcacgc cggcgaacat cgagaacgag ctcaaagcgt
     3301 ccgattctct gatcagtcag gcccatgcgc acggcgacaa gcggaagttc ctggttgccc
     3361 tggtcacctt gggtgcgccc gagttggtcg actgggctcg aaacgaggga agcatcgatg
     3421 ccgctacggc caacaagcac accaaggacc tcagtgaaaa ccctttggca cggagcgccg
     3481 agctccaggc tctacttcgg caactcgcgc acgaccctga agtgcaagcc cgcgtcgtcg
     3541 cttcggtgaa acgagccaac gagagcctgt cccgcgtgga gacaatcaag aagatcttca
     3601 tcttggaccg ggagtttagc gtcgaggagg acgagctcac gccgaccctc aaactcaagc
     3661 ggaagaacgt cgagacgaag ttcaaggaca cgttcgaccg cctctacgac gacgaggcgt
     3721 ttggcttgac cgtcttgtcg gcctaggctc caggctcgat gcgattcgca ctcacatgct
     3781 tgaccgtgat gcttcttgca tcacccgctc tggcgttcga aggcctgctc gaagccagcc
     3841 tctcgagcga gcaaggcgtg gccgccgagg taagcgcccg atactcgaaa gctggcgacg
     3901 ttcggatgga cgtgaagtcc aaggatgccg acgggcagcc tgttcttatg tccacgatca
     3961 tgccggcgaa gggaagctcc tactattcca ttcagcatgc gcaacaaatc atcatcgagg
     4021 taccgtactc gaagctcgcg gagacgagcc agcaggtcac cggctcgggc gacaacgcca
     4081 acctcgagat cgagaagctc ggcaaagcga cggtgagcgg cgtgccgacc gagcacgtcc
     4141 gcgtgatcga caaggacaac gaggccgtca tcgacctctg gatcacccag aaataccctg
     4201 ccgacctctg gacccgtgct tttcgcggcc gaaacctcgg tctagagatg tcagacgacg
     4261 agcggaccaa ggccatgaag aaatacggcg tgaagccagg tttctccatg aagatgaggg
     4321 tagaacaagc aggcggagtg cctgtcgttt tcttggtcaa gaagatcgag cgcggcccag
     4381 tgccagcgga ggtattctcg cttccggaag gatacaagcg cgtccagtct caggggggct
     4441 cgcagccttg acgcgccgcg gagaccttgc gatatacagc gccattagac ggcggtttct
     4501 ggggcgccgg gaggagattt catgactaag caagtgttac tgcgtgcgtt gacgttgttg
     4561 tccgtgctgc tcgttttgaa cggatgcaag tccgacaccg ccaaggatgc caagagctcc
     4621 gatccggtgc tcgtgttgga cgcgggccag gagccacgcg tgccgcttcg ctataaaatc
     4681 gcgcgcggaa ccaccacgac ggccacgatg gacttcggca tcgcatcact cgccacgtcc
     4741 agcagcggag gcgccgctct cacggtgaca cccggtgtgc ggctgcacat cgtctccggt
     4801 ccatcgcttg aaggaaagcg cggagcgacc cgcttcgacg tccggatcgt caaggccgaa
     4861 gcgatcgtgc ccaagggcat cgaccctgcc gtcgcacagg acctcaacaa gagcgcctcc
     4921 gtcctcaaca acgtcgggag ctgggtcgag gtcgacgacc gcggcatcat tcagcgaacc
     4981 cagctcaacg acgcggctca gcggtccgac gtgccggtgc gcttgctcgt gatgatcatc
     5041 aacgcgcgca cgtctcttgc gcgcgtcctt ctccctgccg agccggtggg cgtcggcgcc
     5101 cgctgggagg cgcgaaagga cctgaccttg tacggcttca aggtcaatca agtcgacacc
     5161 tatacgctca cggagcaagt gggcgacgag ctcaagctca acgttcaaat tcagcagacc
     5221 gccgcgccac agacgatcac cttcgaggaa gagggagtcg agctcagcgt cgagtcgttc
     5281 aagatgaacg cggcgggcga agtgatcgcc aacctcaacg cccttgaagc gaacgcagtc
     5341 gctagcggcg actcggaagg cctcatcaat gtgaagacgg tcgacgggac cgaagagatc
     5401 gaaatcgacc gcgcattcca ggtgcggatg accgtgacct atgaggtcgc ccagacagca
     5461 gccgcggccg cagaggcgtc cgaagcggcc caggcagccg cagaggccga gaaggaagcc
     5521 gcaaagaagc tcgaaaaagc tcaggagtag agcgatccag gccagagcgt agccacgacg
     5581 gcacgttgag tccgctctcg tttccagtat tcggattcgg gatcgagctc gacgctgccg
     5641 tcgatcattt cttggagctt ggcttcgatg ccgcgggcga aattcggatc ctgaacgacg
     5701 acgttcgcct cgctctccca gaaactggcg gtcgcgtcga ggttggcaga gccgatgctg
     5761 gttacgaggc cgtccacgga aacgatcttc gcgtgcacgt acgggcgaat gcggccgccc
     5821 cgcgagacga cgttcggaga tggcggcggc acgaattcgt agatccgcac ccccgccaga
     5881 agcagtggct cgagcttggc cttgaccatg tactcgaaaa gcgtgcggtg gagcggcgct
     5941 ggaaagaatg ttccgtcgtc acgccgggca gtcgcgttgc ccgtcagcaa cttcacgctg
     6001 acgtcccgcg caagcaagcg atagatcgcg cgctcgagag caggaatgat cgggaagtcg
     6061 ttcacgatgt aaacgtgttc ctcggcgaga tccaacatcg cttcgtacat cgcgagccca
     6121 ttggtttccg cgagaccgcg gtgcacgatc agccgcccgc acaccgaacc cgcttcctct
     6181 agctccggca tgacgctcga gtcgaccgga atctcagcgc ctccgtgtcg gtgccacgtg
     6241 cgcatgaagg tctcttgcac ttggcgcacc agcggacctg ccacctcgac atgcgcatcc
     6301 agccacggga ttcgttcgtg gcgcgtgttg tcgtgcaccg gcacctcgtc gaagcccagg
     6361 aagtactcgt cggcagcgtt tcgtccggaa acgaaagcgc gtttgccgtc gataatcgtc
     6421 aatttccgat gatctcgctg tttgaaacga gatacggcga cctgcttccc agcgtcgatc
     6481 ggattgagag cgagcacctc gatgttgtct tcttcgcgca gcgactggat gagagggttg
     6541 atgcgcccga acacttcttc atccgagtag agcgcgtcca ccatgaaacg gactttcaca
     6601 cccgcacgcg cggaccgaat cagcagaacg atcagccgtt cggtgaaatc gctcggccga
     6661 accatgtaga gctgaatgtg aacggtcgag gaagcttgct cgagtgctgc gaagagctgc
     6721 ctgcgtgctt ctccgttgtc gagctctgca tgaaacgcat tgccgcaaac gagacgagac
     6781 tcggtggtct ggtcgagtcg ccagtcgagc ggattgctct cgtcgcgctt acgaagcagc
     6841 tcagtcgcct gcttcagctc cgtcacgaag gcgccggaga gagcacgatc gcgcaccgac
     6901 tcgggcgtta gcacgctcac ccgctcctgt tgaatccgct cggaatcctc atcgcgatcc
     6961 gtcaagatcg tcgtgacggg cactcccttc gctcgaaggc gccgtatggc ggtcgaagcg
     7021 aacatgtcga cgaagtcgac gcccatgctc cgaagctcct cctcttcggc cgaatagacg
     7081 agaatcgccc cgggaggcag ccgctgtttg agaagctcgt cgtgaatcag agagtcgcga
     7141 ttcttttcgt ggatatcgaa atagacgagc tcgaagccct cgtcgatcag cgttcgtagc
     7201 gcgtccatcg gggcgcgtct cggtgtcggt tccttcgggg ctccgggaag aaagagctcg
     7261 gcgtgcacca agacgacggg gcggccactg gcgacttgaa ggaggcggtg gccgatcagg
     7321 tccgatacga cctggcctct gtcgttacaa atcttgacgc caacgcgatg aattcctggt
     7381 tcaggcgcta gaatcatcgt gcgaacctct ccgctcggat cgatgggcac ttcgccgacg
     7441 acctcgtcgt ccacgtagaa gcgcgccgtc gtccccaact tggcggacac accgaggcta
     7501 gccgaaagct ccacgcgttc tcccttgctc gtcaggacgt cgcgctgcac accaccgagg
     7561 aagcgtctga gaccggggtt tcgcccccaa cgctccatcg tcgatccgag tgagcgtgct
     7621 gctgcgtcga aaatggagcc ggactcttcg ccggtgagca agcgcttgtc cacctcgccg
     7681 gcgatcgacg ttgcgatttg ccgaatgcgt tctcccaggt cttgcggtgc ctctccgtcc
     7741 gtcacctggc gcagtatagg acgcttacgt atcgtcgtcg gcgatcacgg cgagataggg
     7801 cagattgcgg aacttctggg cgtagtcgag gccgtaaccg acgacgaaga cgtcgtccac
     7861 ggtgaagccg aggtagtcga tttggactgg gaccagggtg cgcgccggct tgtgcagcaa
     7921 ggcggcaacc ctcacggatg cgggctgccg agtcgccagg ttctggtgaa gataggcaat
     7981 cgtgaggccc gtatcgacga tatcctcgac cagaaccacg tgcttgcctt cgatcgaatg
     8041 gctgaggtcg gtagtgatct gcaccacgcc gctgctctct ttcccaccgc cgtagctgcg
     8101 aacgcctaga aaatcgatcg ccacctccat gtcgatctgg cggacgaggt cggctgcgaa
     8161 gacgtagctg cctttgagca caggcacgac caccagctcc ttgccccggt agtcctcgct
     8221 gatctgtcga cctagatcgg cgacacgtgc ggcgatggtg tccgcatcga tcagaggctc
     8281 aatctgcatc acgacttgct cagttcggag agaatacgat tgggaactta gccgggccac
     8341 cttcggacga ggccggaaag cgccagcggc gcaccgaagc ttcgatgcac tccgcaaggc
     8401 ctccaggccc gctgcccgcg gcgctcacgt tcttcacgcg cccggacgaa gccactgtca
     8461 cagtcacatc caagcgtacc gccggtggtg attgcagtcc gcgaatcgct cgctcgtagc
     8521 aacgctggag gcgcggcttg ttctccgtca cggtcgcgcg aaccccgtcg ccatcgagtg
     8581 gtgggttttc gctctgcgca gtgctcgcct tctcgacttc gatcttggca ggtgccgcat
     8641 tggcgttcga gcggaactcg gcgagaacct tctggtcggc gtcgctcacc gaatcgcgct
     8701 cgggagcaac gggctccgcg accggctctg gcttcggagc ggctctgcgc tcggccctgg
     8761 ctttcggtct cggtgtcggc gcgatcttcg gaccgtcatc gcggggattc ggcggctcga
     8821 ccgcgagctt accgtcgtcg ccctctacgt tctcctcctg gggagccccg tcttgttcga
     8881 cggcctcgac ggcttcaacc gcgccgacgg cctcaccctc cacgagctca gcggattcgg
     8941 ccgcgccgac cggctgctcc tcggctgcca gatcggcttc gggagccggc ttttcggcgt
     9001 tcattgcagc cctggtcgaa ggacgggaag gcgccgaagc ggagaccggc tcagtccctt
     9061 cgagcagatc tcgcagcaag gagtcaccgt agcgctcgaa ggctagaaac gcgacgactg
     9121 cggaaaacac ggtcactgcg atgatcaccg ccacgagtcc aagtgaccgc ccgcggcgcg
     9181 gttcgaccga agcgagcaca gctttcgggg gcacggaggg aacggcaaca gtctcgtccg
     9241 gccctgccgg gaagcctgaa aaataaccga gctgaggcga agagccgaag acattggacg
     9301 ggccaagggg agccggcggt tgcgacatct ctttgaagat gtccggctcc gagccttccg
     9361 gttgcagcga atcggcaagc ggggcgaagt ccatgcccat ggccatgcca gagctcgagc
     9421 gaacttcagc cgcagcgacc gttgcagcaa aggccacgga gtcgtcgacc gaggtgcgcg
     9481 aatcgacgaa cggtggcatc gaggagaacg a
//
LOCUS       DASRZF010000282         7382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2963290, whole genome shotgun sequence.
ACCESSION   DASRZF010000282 DASRZF010000000
VERSION     DASRZF010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 7382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7382
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2963290"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VFG22_12300"
     CDS             <1..316
                     /locus_tag="VFG22_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417069.1"
                     /translation="ISTILAAMKQALKLLFVMTLGAFLLTACGDDNPINGVGTVCASD
                     DASVGDTATCPDSTEVIDFCINGGNGSCYYVVGGEQVNCGNCFDGGSITTCAQEAIAL
                     CP"
     gene            complement(349..690)
                     /locus_tag="VFG22_12305"
     CDS             complement(349..690)
                     /locus_tag="VFG22_12305"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET6417070.1"
                     /translation="MKRGARHPRLLQNLPASLDEEQIDELLRTPNLRIERIVSTGQAS
                     AEETWYDQSEHEWVIVLSGSAKLRYEDEEGEVELGPGDAAFIPAHRKHKVAWTSPKEP
                     TVWLALFWTEP"
     gene            complement(797..1120)
                     /locus_tag="VFG22_12310"
     CDS             complement(797..1120)
                     /locus_tag="VFG22_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417071.1"
                     /translation="MFRSEVGRHLIALIAVAVAVFLQNSATSGVDAHRGGSPEPSGLV
                     DFATISPEAPAGARDHDGAQLLGSDLAVPVLPALDNGSRLPLQVAEPMRRSSVEESKH
                     KLSFL"
     gene            1338..2114
                     /locus_tag="VFG22_12315"
     CDS             1338..2114
                     /locus_tag="VFG22_12315"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HET6417072.1"
                     /translation="MKLAYWVLILGAGVAVYGCGDDGGGAGSAGTAGTGGDGGTGGVG
                     GGGPNVPTEGLQAFLSFSGDASDESGNGNDGTLLGGATASGELILGNNADDMLMLPSA
                     VMDGLVDFTFAAWLRIDTIRGESHEVISGANAAEDNALSFWYRDHTDEWVVGINNGSS
                     VLANDATIEDGEWHHVALTRSGTVAVLYIDGSQLGNAEDVGADTLDIDPGGLIFGQDQ
                     DEVGGGFEADEAWAGAMDNLRIYDRALEQVEVALLTAEAR"
     gene            complement(2185..3474)
                     /locus_tag="VFG22_12320"
     CDS             complement(2185..3474)
                     /locus_tag="VFG22_12320"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET6417073.1"
                     /translation="MDLGSGVGLVVANMIGAGVFLSAGFMAQELSAGWILSAWLVGAL
                     LAMAGARTYAEASVLIPRSGGEYRYLSELIHPAVGYLAGWASLLVGFSAPMAISAYAA
                     AAFAFAVFGAGEPRLGATAVIIALTLFHSLGLKLSKWTQNGLVIVKGLLVLGFVALGL
                     SIGSTDWPTWVPASGNTDFAPLPFATSLFFVAYAFSGWNAAAYTAEEFRNPKRDVPRA
                     MLIGCAVVGLVYLLVNWVLVANLTPERASVVFSYEEARVTLGHLIAQDLLGEAGSGVM
                     SMGISIVMLSSVSAMLLVGPRVYAEMARDGFLPAVLRGRPGIPPRASIAIQGALAIVL
                     LYLHSVGELLSNVAGVLIFFSALVALGLFFVKRRKPHLPAPRKSALFAAAVYAGSSVW
                     MLYNAFEAKTSLLPWLGVLAVVGLAGYLVTDRRRNAR"
     gene            complement(3586..4056)
                     /locus_tag="VFG22_12325"
     CDS             complement(3586..4056)
                     /locus_tag="VFG22_12325"
                     /inference="COORDINATES: protein motif:HMM:NF021435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HET6417074.1"
                     /translation="MIRQMRTLVFTLSSLAVMPALAQEAEPSAEAKPANEIVVEIIGL
                     ENDNGQIGCRIFSKADGFPGKMNKADEQLYSKPKSKKGSCTFKGYKPGTYAISVMHDL
                     DSSGDLNTSMVGKPKEPWGVSNDAPAHRFGPPDFEECTFKYEGGKKFIKIKLQH"
     gene            complement(4153..5166)
                     /locus_tag="VFG22_12330"
     CDS             complement(4153..5166)
                     /locus_tag="VFG22_12330"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6417075.1"
                     /translation="MNWYRIKTALVGLKRLTELSDADKQACIDAFKFLQRMQDGEPTE
                     TADETKAVADYYKVLNNMLSVFDLEKLYIPPQLDESQGLYGNQLLVEQAVLKELDLRD
                     PKSSHLLDMGCGRGRVSHHFATLTGGHVSGYNIDPNQIENAIDWAAECGMSDRLHFKV
                     GNHHEPLEYDSETFDGCFSFQAVWPFFKKEELDDHAGEMYRVLKPGARYACSEYLLTP
                     HFDWNNEEHVSLHKAFLPTLAATQSMYPADVCAALERAGFTIVLSAPSKSAAWPICEQ
                     KRDLILGGRKVIRALEAIRVLPPWVEESLDLLQSGGQAWTDAEKAKIADLNWRIVAEK
                     PLA"
     gene            5346..7001
                     /locus_tag="VFG22_12335"
     CDS             5346..7001
                     /locus_tag="VFG22_12335"
                     /inference="COORDINATES: protein motif:HMM:NF005760.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HET6417076.1"
                     /translation="MANGAHALIQTLVNAGVDTCFTNPGTSEMHFVAALDDADMRAVL
                     CLFEGVASGAADGYARMADKPAATLLHLGCGLGNALANLHNARKARVPMVNIIGDHAT
                     YHTQYDAQLQSDIETVARNISTWIKTTPKSSLLAADAAEAVAVAKGPPAQIATLILPA
                     DVSWNDGAEPAQAHAPVQPPPASDATVQALAEVIKKSGKKVAFLLGNRVLREAGLRAA
                     SQIAEKTGAKLFCEVFPTRLERGAGLPAVERIAYLAELASVQLDGYDHLVLVDAKAPV
                     SFFAYPGKKSYLVPDDCTAHTLVQPHEDALASLLALVEAVGASDTAPKLQSAGRPDLP
                     KGKLNAAKACQAIGALLPASAIISDEAQTSGIKLPTFTAGAPRHDVLTLTGGAIGQGL
                     PVALGAATASPNRPVLALVGDGSAMYTIQALWTIAREGLDVTTVIFNNRSYGILNIEL
                     ERVGAETIGPEAKSQLDLSEPAIDFVSMAQGMGVPTRRVARAEDFSAALKNAFDSHGP
                     FLIEAIVPSEYEGLKLKALPHMLSALSSIPSPVAKAIKKKIAP"
     gene            complement(7018..>7382)
                     /locus_tag="VFG22_12340"
     CDS             complement(7018..>7382)
                     /locus_tag="VFG22_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417077.1"
                     /translation="LKPGGRAIFYEVCAHKNTSIRFPVPWAQDSSMSFLVPPESFRDI
                     IASAGFEIAVWNDKTDLAKQAFANAREPVGEPNLPILGVYLLVGSDIQTKAYNLRRNL
                     EDESVSLIESVALKPSLD"
BASE COUNT         1474 a         2261 c         2213 g         1434 t
ORIGIN      
        1 aatttccacg atactcgccg ccatgaagca agctctcaaa ctcctcttcg taatgaccct
       61 gggcgccttc ctgctcaccg cttgcggaga cgacaatcca atcaatggtg ttggtaccgt
      121 ttgcgcgtca gacgacgcga gcgtcggcga cacggccacg tgcccagaca gcacagaggt
      181 catcgacttc tgcatcaacg ggggcaacgg cagttgctac tacgtagtcg gcggcgaaca
      241 ggtgaactgc gggaattgct tcgacggcgg atcgatcacc acctgcgcgc aagaagcaat
      301 cgccctctgc ccttagtagg ccgagcactc ccctcctctt tacgcacctc agggttcagt
      361 ccaaaacagg gcgagccaga cggtgggctc ctttgggctc gtccatgcca ccttgtgctt
      421 ccggtgagcg ggaatgaacg cggcatcgcc cggcccgagc tcgacttcgc cttcctcgtc
      481 ttcgtagcgt agcttcgcgc tgccgctcag cacgatcacc cattcgtgtt cggattggtc
      541 gtaccaagtt tcctccgccg atgcttggcc ggtggagacg attcgttcga tcctgagatt
      601 gggcgtccga aggagctcat cgatctgctc ttcgtcgagc gacgctggga ggttttggag
      661 caaccgagga tgtcttgcgc cgcgcttcat gggtcttctc gaatcgtact cgatctgggc
      721 atagaagatc tcttgcccgc gagcgcttcg agtagggcga gtcgacgctc cggaccgaac
      781 cctcgactcc ggttagctag aggaagctca gcttgtgctt ggactcttcc accgaggacc
      841 gtcgcattgg ctccgcgact tggagaggga gccgcgaacc gttgtcgagt gctggcagaa
      901 cgggcaccgc caagtcgctc ccgagcaact gagccccgtc atggtctcgc gcgcccgctg
      961 gcgcctcggg cgagatcgtc gcgaaatcga cgaggccgga cggctccgga ctcccgcccc
     1021 gatgagcgtc cactccagac gtggctgagt tttggagaaa gaccgcaacg gcgactgcga
     1081 tcaaggcaat caaatgtcgc ccaacttccg atctaaacat gagcttcctc ctttttcgga
     1141 ccacagcagc aaagatcgtc ccgaaccgtt gagttgacaa tccagataat tcgccgctca
     1201 catgtagatt ttatctacat agctcgcgcc tgagcaggcc cgcgcaaacc ggcgcaccca
     1261 cgttcatttt gtcttggatc attctggctc gagaagacta gtctcttgct ttgagaacgc
     1321 ctgctgggag gttggtcatg aagctcgcat attgggtgct aatcctaggg gcgggagttg
     1381 ccgtctacgg ctgtggggac gatggcggcg gcgcaggaag cgctggcacg gctgggaccg
     1441 gcggcgacgg tggaacgggc ggtgtgggtg gagggggccc aaacgttcca acggagggcc
     1501 ttcaagcctt tctgtcgttc agtggggatg ccagcgatga aagcggcaat ggtaatgacg
     1561 ggacgctgct tggcggggcc accgcatcgg gggaactgat cctcggaaac aacgcagatg
     1621 acatgctgat gcttccgtcc gcggtgatgg atggcctggt tgatttcacc ttcgcggcct
     1681 ggctcaggat cgacacgatt cgaggcgaga gccatgaggt gatcagcggc gccaacgccg
     1741 ccgaggacaa cgctctgagc ttttggtacc gcgaccacac ggacgagtgg gtggtcggaa
     1801 tcaacaacgg ctcctcggtc ctcgccaacg acgcgacgat tgaagacggc gagtggcacc
     1861 acgtcgcgct cactcgctct ggcaccgttg ccgtactcta catcgacggt tcacagctcg
     1921 ggaatgcgga ggacgtcggt gcggacaccc tcgacatcga tccgggtggg ctcatcttcg
     1981 ggcaggatca ggacgaagtc ggcggaggct tcgaggcgga cgaagcctgg gccggagcga
     2041 tggacaacct gaggatctac gaccgagctc tagagcaagt ggaagtcgcg ctgctgacag
     2101 ccgaggctcg ctagggctgg cctgccattg gctgtgatcg ctggtcacgg agggtcacgc
     2161 aggggctcat gcggaagttg aacctcatct agcgttgcgc cgccggtcgg taaccagata
     2221 gccggccaag ccaacgactg ccaacacgcc gagccaagga agcaagctcg tcttggcttc
     2281 aaacgcgttg tagagcatcc acaccgagga cccggcatac acggcggcag cgaatagggc
     2341 cgatttccgc ggcgcgggta ggtggggctt gcggcgcttc acgaagaaca ggccgagggc
     2401 cacgagcgcc gagaagaaga tcaagacccc ggccacattg ctcaggagct cgccgaccga
     2461 gtgaagatag agcagcacga ttgcgagtgc gccttgaatg gcgatcgacg ccctcggtgg
     2521 aatgccaggg cggcctcgga gcacagctgg gaggaagccg tcgcgagcca tctctgcgta
     2581 gacgcgggga ccgaccagca gcatcgcgct cacgctcgac agcattacga tggagatgcc
     2641 catcgacatg acgcccgagc ccgcttcgcc gagcaggtcc tgcgcgatca agtgccccaa
     2701 ggtgactcga gcctcttcgt agctgaagac cacgctagcc cgctcgggcg ttaggttcgc
     2761 caccagcacc cagttgacga gcaagtatac gaggccgacg acggcgcacc cgatcagcat
     2821 cgccctcggg acgtcgcgct ttgggttgcg aaattcctcc gcggtgtacg cagcggcgtt
     2881 ccaacctgaa aacgcgtatg cgacgaaaaa gaggctcgtc gcaaacggaa gcggggcgaa
     2941 atccgtgttt ccgctcgcag gtacccaggt tggccagtcc gtgcttccga tcgacagtcc
     3001 gagcgccacg aatccgagga cgagcaagcc cttgacgatc acgaggccgt tctgagtcca
     3061 cttcgagagc ttcaagccaa gtgagtgaaa caaggtcagc gcgatgatca cggcggtggc
     3121 gccgagacgg ggctcgcccg cgccgaacac ggcaaacgca aacgcggccg ctgcgtaggc
     3181 actgatcgcc atgggcgccg aaaagcccac cagtagagac gcccatccgg ccaagtatcc
     3241 tacggccggg tgaatcaact cggacaagta gcgatactcg ccgccggagc gcggaatcag
     3301 aacactcgcc tcggcgtacg tccgcgcgcc cgccattgcg agcagggctc ccacgagcca
     3361 ggcagacaag atccagccgg cgctgagctc ctgtgccatg aagccggcgc tcaggaacac
     3421 acccgcgcca atcatgttgg ccaccacgag cccgaccccg gatccgaggt ccaagaaccc
     3481 ttcgcccgga ggaggcgcat atgaatcaag agtttctcgc gccaccctac tccgtgacga
     3541 gcgatcctag cgattgttgg tccgaacgcg aaataggcga ccggactagt gctgcagctt
     3601 gatcttgatg aacttcttcc cgccttcgta cttgaaggta cattcctcga agtcaggcgg
     3661 gccgaaacga tgtgcaggcg cgtcgttgga cacgccccag ggttctttcg gctttccgac
     3721 catggacgta ttgaggtcac cgctgctgtc aagatcgtgc atgaccgaga tcgcgtacgt
     3781 gcccggcttg tagcctttga acgtacagct gcccttcttc gatttgggct tgctatagag
     3841 ctgctcgtcg gccttgttca tcttgccggg gaagccgtca gctttcgaga agatccggca
     3901 gccgatctgt ccgttgtcat tctcgaggcc gatgatctcg acgacgatct cgttcgcagg
     3961 tttcgcttcc gccgacggct cggcttcctg cgcaagcgcc ggcatgacag caagcgagct
     4021 gagcgtgaaa accagtgtcc tcatctgtcg gatcatcgtg cgtcctcctg tcgcgagata
     4081 gggtgtctac acgccctatc ttcagctcga cgagtgtagg tcaaaggggt tgtgcgcaag
     4141 atataaagca gcctaggcca gcggtttctc tgcgacgatt ctccaattca aatcggcgat
     4201 tttggccttt tccgcgtcgg tccatgcctg gccgccgctt tgaagaaggt cgagcgactc
     4261 ttcgacccag gggggcaaga cgcgaatcgc ctcgagcgcc ctgatgacct ttcgcccgcc
     4321 gagaatcaaa tccctcttct gctcgcaaat cggccaagcc gcgcttttcg aaggtgctga
     4381 aaggacgatt gtgaaccccg ccctttccaa ggcggcacag acatcggctg gatacatgga
     4441 ttgagtggcc gcgagcgtgg gcagaaatgc cttgtgcagg ctcacgtgct cttcgttgtt
     4501 ccagtcgaaa tgcggcgtca atagatactc ggaacacgcg tatcgagcgc ctggcttcaa
     4561 cacgcggtac atctctccgg cgtgatcatc cagttcctct ttcttgaaga acggccagac
     4621 cgcttgaaag gaaaagcacc catcgaacgt ttccgaatcg tactcgagtg gctcgtgatg
     4681 gttgcccacc ttgaagtgca gccgatcact cattccgcat tccgccgccc agtcgatcgc
     4741 attctcgatc tgattggggt cgatgttgta tccggacaca tgaccgcccg tgagcgtggc
     4801 gaagtggtgt gagactctcc ccctcccaca ccccatgtcc aacagatgcg agctcttggg
     4861 gtctcttaga tcgagctcct tcaatacagc ctgctcgact aaaagctgat tcccgtacaa
     4921 tccctgggat tcgtctagct gcggtgggat gtagagcttc tccagatcga acaccgagag
     4981 catgttgttc aagaccttgt agtagtccgc gactgctttg gtttcgtccg cggtctcggt
     5041 tggctcacca tcctgcattc tctgaaggaa cttgaaggca tcgatgcagg cttgcttgtc
     5101 cgcgtccgac agctccgtca atcgcttgag tccgaccagt gcggtcttaa ttctgtacca
     5161 attcacttgc tagacctctc attgaacacg ttcgcagtcc tccagcgggc aagtccagcg
     5221 cgctacatag ccctcacgtc cagatcacgc cagaagctcg tcaggggcga tctcctcggc
     5281 cgtcgcgcgg ctcttgtttt cttgggagct ctcgaggcgg cgtatgtagt acagtgccga
     5341 gcaccatggc caacggcgca cacgctctga ttcagactct cgtcaacgca ggcgttgaca
     5401 cctgttttac caatcccgga acttcggaga tgcacttcgt ggcggcgctc gatgacgcag
     5461 atatgcgagc tgtactctgc ttgttcgagg gtgtggcctc gggcgctgcc gacggatacg
     5521 cgcgcatggc cgacaagcca gccgccactc tgttgcatct cggatgcggg ctcggcaacg
     5581 ccctcgcgaa cctacacaac gcacgcaagg cacgtgtgcc catggtcaac atcatcggag
     5641 atcacgctac ctatcacacc cagtacgacg cgcagttgca gtcggatatc gagacggtcg
     5701 cgaggaacat ttctacgtgg atcaagacga cacccaaatc gagtctactc gccgccgatg
     5761 ccgccgaagc tgtggcggtg gccaaaggcc cgcctgcgca gatcgctacg ctcatcttgc
     5821 ccgctgatgt gtcctggaac gacggcgccg agccggcaca ggcgcatgcg cccgtccaac
     5881 cgccgccggc ctcggacgcg acggtgcagg ccctggctga ggtgatcaag aagtccggca
     5941 agaaggtcgc gttcctgttg ggcaatcggg tcttgcgcga ggcaggcttg cgcgcggcat
     6001 cacagattgc cgaaaaaacg ggcgccaagc ttttctgcga ggtctttccg acgaggctgg
     6061 agcgaggcgc cgggctgccc gccgtggagc gcattgcgta tctagccgaa ctggcctctg
     6121 tgcagctcga cggctacgac cacctggttc tggtggatgc caaggcgccg gtctcgttct
     6181 tcgcgtatcc gggcaagaag agctatttgg tgccggacga ctgcacggcg cacaccttgg
     6241 tgcagcccca tgaagatgcg ctggcctcgc tcctggctct cgtcgaggct gtcggtgcgt
     6301 ctgacaccgc acccaagctg cagagcgccg ggcgtccgga cttgccgaag ggcaagctca
     6361 acgctgcgaa ggcgtgtcag gccattggtg cattgttgcc ggcaagcgcg atcatctcag
     6421 atgaagccca gacctcaggt atcaagctgc cgaccttcac cgcgggggcg cctcggcatg
     6481 acgtgctcac cttgaccggt ggcgcgatcg ggcaaggcct gccggtcgcc ttgggcgcgg
     6541 cgacggcctc gccgaaccgt cccgtgctcg cactcgttgg cgacggctcc gccatgtata
     6601 cgattcaggc tttgtggacg attgcgcgtg agggtctcga tgtgacgacc gtcattttca
     6661 acaaccgctc ctacggaatt ctcaatatcg agctcgagcg cgtcggggca gagacaatcg
     6721 gcccagaggc aaaatcgcag ctggacctgt cggaaccagc aatcgacttc gtgtcgatgg
     6781 cccagggcat gggcgtcccg acacgacgcg ttgcacgggc cgaggacttc agcgcagcac
     6841 tgaagaacgc attcgactcg catggcccgt tcttgatcga ggcgattgta ccgagcgagt
     6901 acgaaggcct gaaactcaaa gcgctgccgc acatgctaag cgcgcttagc agcatcccat
     6961 cccccgtcgc aaaggcgatc aaaaagaaga tcgcaccctg agccgcgacg aagacgatta
     7021 atcaagactg ggcttcaacg caacgctctc gatcaggctg acggattcgt cttctagatt
     7081 gcgacgcagg ttgtaggcct ttgtctggat gtcgctgccc accagtaagt acacgcctag
     7141 aatcggcaga ttcggctctc ccaccggctc cctcgcattt gcaaacgcct gtttcgcaag
     7201 atcggtcttg tcattccaca ctgcaatttc aaatccagcg gatgcaatga tgtcgcggaa
     7261 cgattccggt ggcaccagga agctcatgga gctgtcctga gcccagggca cgggaaagcg
     7321 aatactcgtg ttcttgtgtg cgcatacttc ataaaagatc gcacgcccgc cgggcttgag
     7381 ca
//
LOCUS       DASRZF010000283         2255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1588513, whole genome shotgun sequence.
ACCESSION   DASRZF010000283 DASRZF010000000
VERSION     DASRZF010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1588513"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VFG22_12345"
     CDS             <1..409
                     /locus_tag="VFG22_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET6417078.1"
                     /translation="LQRPVRAINDADLQGLGVVRGEGVELVLTLGTGMGAALFTDGAL
                     VPNLELGHHPMRDGMTYEDLVRDSEFKRIGPAEWTARVIEAIGQIEALFNYDTLHLGG
                     GNARNLRSILPANVRVFTLAEALCGALKVWDDA"
     gene            complement(406..1047)
                     /locus_tag="VFG22_12350"
     CDS             complement(406..1047)
                     /locus_tag="VFG22_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6417079.1"
                     /translation="MIIEHFSVAPLGCNCVILGDEERGTAIVVDPGGEAPRILARLEE
                     LGLKCEAIVHTHTHFDHIGATKQVQDATGAPTMIHEDDLFLYEGVQMQLDSFRAPFRA
                     PEVAEIDRFLSDGDTVQAGSLEADILHTPGHSPGSICLTIEGDRPVLIAGDTLFARSV
                     GRSDLWGGDGAQLVDSIRRKLFALPDETLVICGHGPNTTIGEERRQNPFVRNA"
     gene            1191..1472
                     /locus_tag="VFG22_12355"
     CDS             1191..1472
                     /locus_tag="VFG22_12355"
                     /inference="COORDINATES: protein motif:HMM:NF018563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HET6417080.1"
                     /translation="MLDVNEYFDGNVKSIGFQTETLRATVGVMAPGEYEFGTSRKETM
                     TVVSGALTVRLPGSEEWLRFDPGSSFVVDAKRKFQLRVTVDTAYLCTYE"
     gene            complement(1500..>2255)
                     /locus_tag="VFG22_12360"
     CDS             complement(1500..>2255)
                     /locus_tag="VFG22_12360"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HET6417081.1"
                     /translation="SSTEGSLRFLKIHETRRTGVFFEAVMRRSVRAAPALALLLSCAT
                     GCDAILTWTTERWTERFPEASESSEPSVTPEDGAPEIAEAEVTERPADSIARSAEGVS
                     RFAAILEDETMTREERIRAFENLDPAALQAPAKPNVVAGGAPAQSQARNSDSRAPKDW
                     EIANARKRVPIVMFSTTWCGVCKRAKAYFEQEGIAFVEHDVDQNQAARAEYLALNPKK
                     TVPTIKIGNEVVIGFSEQAVQAAIDAAARARVR"
BASE COUNT          384 a          710 c          706 g          455 t
ORIGIN      
        1 gctccaacgc cccgtgcggg ccatcaacga tgccgacctc cagggtctag gggtcgtgcg
       61 tggagagggc gtcgagctcg tgctgacctt gggcaccggc atgggtgcag cgctcttcac
      121 cgacggagcg ttggttccca atctcgagct tggtcaccat ccgatgcgcg acggtatgac
      181 atacgaagat ttggtccggg acagcgagtt caagcgcatc ggccctgctg aatggaccgc
      241 ccgcgtcatc gaagccatcg ggcagatcga ggcgctcttc aactacgaca cgctccacct
      301 ggggggcggc aacgctcgca accttcggtc gattctccct gccaatgtgc gtgtgttcac
      361 gctcgcggag gcgctctgcg gcgcgctcaa ggtttgggac gacgcttagg catttcgcac
      421 gaacgggttt tggcggcgct cttcgccgat cgtggtgttc gggccatgtc cgcagatcac
      481 gagcgtctcg tcgggaagcg caaagagctt gcgccgaatc gaatcgacca actgggcgcc
      541 gtctccaccc cagaggtcgg aacggcccac gctgcgcgcg aagagagtat cccctgcgat
      601 gagcaccggc cgatcgcctt cgatcgtgag gcagatactt cctggtgagt gtcccggcgt
      661 atgcaggatg tcggcctcga gggagcctgc ttgcaccgtg tcaccatccg agaggaagcg
      721 gtcgatctcg gcaacctccg gggctctgaa cggtgctctg aagctatcga gctgcatctg
      781 cacgccttcg tagaggaaca ggtcgtcttc gtggatcatc gtaggcgcgc cggtggcgtc
      841 ctgcacttgt ttcgtggcac cgatgtgatc gaaatgcgtg tgcgtatgca cgatcgcttc
      901 gcacttcagt ccgagctcct cgaggcgcgc cagaattcgc ggcgcctcac cacccgggtc
      961 gaccacgatg gccgtgccgc gctcctcgtc gcccaggatc acgcagttgc agccgagggg
     1021 cgccacggaa aaatgttcga tgatcatcgg gcccttctag caccgctcgc cctgcctgac
     1081 accagcatgc gcccgtgtta tctccacgct gaacgttttc ggtcacaccc cgcgcccaac
     1141 cagtgcgggc acgccgacac cgtcgagaag acctgccacg gaggatgagc atgctggacg
     1201 tcaacgagta cttcgacgga aacgtgaagt cgattggatt tcagaccgag acgcttcgcg
     1261 cgaccgtggg tgtgatggcc cccggcgagt acgagttcgg cacgagtcga aaagagacca
     1321 tgacagtcgt aagcggtgca ctgaccgtca gactacccgg ctccgaagag tggctgcgtt
     1381 tcgatccggg ttcgagcttc gtggtcgatg ccaagcggaa gttccagctt cgggtgaccg
     1441 tcgataccgc gtatctctgc acctacgagt agggcggcgc tggaaatttc gtcgcgcgct
     1501 cagcggacgc gcgcgcgcgc tgctgcgtcg atcgcagcct gaaccgcttg ctcgctgaag
     1561 ccgattacga cttcgttgcc gatcttgatc gtcggaaccg tctttttggg gttgagggcc
     1621 aggtactcgg cgcgcgcagc ttggttctgg tcgacgtcgt gctccacaaa cgcgatgcct
     1681 tcttgctcga aataggcttt ggctcgctta cagacaccgc accaggttgt cgagaacatc
     1741 acgatgggaa cgcgtttgcg tgcgttcgca atctcccagt ccttgggcgc tcgtgagtcg
     1801 ctgttccgtg cctggctctg cgccggggcg ccaccggcga ccacgttcgg tttcgcaggt
     1861 gcttgcagcg ccgccggatc gaggttctcg aaggccctga tgcgctcctc ccgcgtcatc
     1921 gtctcatctt cgagaattgc ggcgaagcgg ctcactccct cggctgaacg tgcgatcgag
     1981 tctgcgggcc gctctgttac ctccgcctct gcgatctcgg gagcgccatc ctcgggcgtc
     2041 acgcttggct cgctcgattc cgacgcctcg gggaagcgct cggtccaccg ttccgtcgtc
     2101 catgtaagga tggcatcgca acccgttgca cagctcaaaa gaagggctaa ggccggtgcg
     2161 gctctgacgg atctgcgcat gactgcctcg aagaatacgc cggtgcgccg cgtttcgtga
     2221 attttcaaga acctcaggga gccttcggtg ctcga
//
LOCUS       DASRZF010000284        10709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1934545, whole genome shotgun sequence.
ACCESSION   DASRZF010000284 DASRZF010000000
VERSION     DASRZF010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 10709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10709
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1934545"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(313..1185)
                     /locus_tag="VFG22_12365"
     CDS             complement(313..1185)
                     /locus_tag="VFG22_12365"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HET6417082.1"
                     /translation="MILELLSVVAPVFVVALVGLVWARSGAHFDEVSISRLVLNVGIP
                     CLVFRSLTSLDVPPAELARMAGLAAAAMSIFAGAGYLVLKAMRLPAHTFLGPLAFSNS
                     GNIGLPICLFAFGEAGLALGMAYFAVSSTCHVVLGGPLFSGSFSARPFFRSPLTWAVL
                     ITVVVIASGIAIPTWVERTTTLLGDIAIPLMLLTLGVSLSKMHPRSLGRSVTLSFVRL
                     GLGLGAGLLLTALFGLEGLTRKVLVLQASMPVGVLNYLFAQRYGRSPEQIASLVLVST
                     LVSVISIPVLLAWL"
     gene            complement(1192..3018)
                     /gene="cysN"
                     /locus_tag="VFG22_12370"
     CDS             complement(1192..3018)
                     /gene="cysN"
                     /locus_tag="VFG22_12370"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF004035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HET6417083.1"
                     /translation="MTDEKELIRDDIEAYLEKYRKKELLRFVSVGSVDDGKSTLIGRI
                     LYDTGMVFEDQMAAVRAASKGDEPDLALLTDGLKAEREQGITIDVAYRYFSTDKRKFI
                     IADTPGHIQYTRNMVTGASTADVALILIDARHGVLQQSRRHAYIASLLGIPHLAVCVN
                     KMDLRDYSEKVFDEISADFTEFGKTLRFKDIQFFPVSALVGDNVVSDSENTPWYDGPN
                     VLEYLETVPVFADRNFEDFRYPVQYVIRPDLDYRGFAGEVAAGVIAKGDEIVALPSGK
                     TSRVKAIDTYDGELDEAFASQSVTIRLEDEIDISRGDMIVKPGNLPRVTRRFDAHLVW
                     MHEKPLDTEKAYLVKHTTQTVRAQIDRIYFEVDMDTLEEKPTDGLGLNDIAKVRLSCH
                     RALYVDDYQRNRETGAFIVIDSLTNNTVAAGMISLAGSEQNIGEVMKELHAESAMEPK
                     TFVSPNERMERFGQRGATVWLTGLPGSGRWALAYALERKLFDEGRTATVISPVGEDLR
                     SMISAAKAITDAGLINICAFPSPTRTDRDKLKERIGGDRVIQVYVNTDVALCRERRPD
                     ADFSSFEPPDDPDIIVALDEMRIDKAVETILEALKERGQFEA"
     gene            complement(3049..3963)
                     /gene="cysD"
                     /locus_tag="VFG22_12375"
     CDS             complement(3049..3963)
                     /gene="cysD"
                     /locus_tag="VFG22_12375"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF009214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HET6417084.1"
                     /translation="MQTSEHKLSQLRQLEAESIQIIREAAAEFDNPVMMYSVGKDSSV
                     MLRLAEKAFAPGGLPFPLLHVDTTWKFQDMYRYRDKIEANLKGELIKYTNPEGVAMGV
                     NPFDFGSKKYTDIMKTEALKQALTKYEFDCAFGGARRDEEKSRAKERIFSFRDSHHQW
                     DPKNQRPELWNVFNAKIKKGENVRVFPLSNWTELDVWLYVWLENIPVVPLYFAEVRPM
                     VERSGTWILVDDDRMRLEPGEKPQMKKVRFRTLGCYPLSGAVESEADTVPKVIQEMLL
                     NRFSERQGRLIDFDEEGSMEVKKREGYF"
     gene            4089..4634
                     /gene="orn"
                     /locus_tag="VFG22_12380"
     CDS             4089..4634
                     /gene="orn"
                     /locus_tag="VFG22_12380"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HET6417085.1"
                     /translation="MSAIVWMDMEMSGLDPETERILEIAVLVTDGELNVVAEGPNLVL
                     HQPDAVLEAMDEWNTRHHGQSGLTQRVRESTVDEAQASAALMDFLSHHTEKNKAPLAG
                     NSIHQDRRFVAKYLPEVEEWLHYRNVDVSTIKELSQRWYPKQYSARPSKKGSHRAMDD
                     LMESIEELKYYRAAVFLPSES"
     gene            4631..5527
                     /locus_tag="VFG22_12385"
     CDS             4631..5527
                     /locus_tag="VFG22_12385"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HET6417086.1"
                     /translation="MKLAALSLLVCMGCAGAMKPETRTAESIVRSESAPASLVDAATG
                     KEIDLETLLAAVRTKQVLYVGERHDQPLDHNVQYSILRHLHREEPSLAIGMEMFQTPY
                     QQPLDEWRAGRIDETVLRRETEYDKRWGFDFSMYRPILEYARSNGIEVVALNAPRELA
                     YAVAKDGVDGLSPEQAAMLPKLDLGNEQHRALFDEEFDLGDHAAQAEIDLYYQAQVVW
                     DETMASRVAEALGRANGPSKMIVLAGRVHVKRGLGIPDRASKRGAAPYAVVMPVTRQE
                     LKAELKLPTEGRSADFFWVVEQ"
     gene            5647..6858
                     /locus_tag="VFG22_12390"
     CDS             5647..6858
                     /locus_tag="VFG22_12390"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HET6417087.1"
                     /translation="MTDCFIYDAVRTPRGKGRKKDGALADAPPLRMAKTVLRALPERG
                     NFDSKEIEDVIFGCVEPVGEQGANVGRMAALIAGYDETVPGVQLNRFCSSGLEAVNIA
                     AAKVKAGDVDLIVGGGMESMSRISMGAAGGAWSTDPALAWGTSFVPQGIGADLIATKH
                     GYSREDVDSFAVESQRRAAAAWEGGHFTKSIVPVKDDVGVTMLDRDEHMRPGTTLEDL
                     AQLKASFEVMGRNYGFDAIAIQKHPEVEQIHHVHHAGNSSGIVDGAAAVLLGSKEAGK
                     RLGLKPRARIVRSATVGSEPCIMLTGPAPASRKALTKAGMRPSDIDLWEINEAFSSVC
                     LLFMEEMGIDRSVTNVNGGAIAFGHPLGATGAMLLGTMVDELERRDLNTALITLCVGA
                     GMGTTTIIERV"
     gene            6863..9049
                     /locus_tag="VFG22_12395"
     CDS             6863..9049
                     /locus_tag="VFG22_12395"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HET6417088.1"
                     /translation="MAIIDYRVDDGVAILTWDDRDRPMNVLSGDALAELGAGVEKAVA
                     DDAVKGIVLTSGKQGVFVAGGDLQQIETLGSGPIDAADLLKRTGAVLDQLRRMETCGK
                     PVVAALNGVTLGGGYEIALACHRRILVNQRHAQVGLPEAGLGLMPGAGGTQRLPRLIG
                     LQGALGLIMQGTQLSPDQALEKGLVDELVEPPELIPAAKKWIHENGSATQPWDEKRYQ
                     VPGGGADSVTFAQTMMGANAMTHANTYGNILSQEAILQSVYDGLGLPMDRAGKVETRQ
                     FIRVLQDPTARSMLRTLFFSLQECQKGARRPEGVPRFDVNKVGILGAGLMGQGIAHVS
                     AKVGIEVVLLDRDLASAQRGKDKVAKTLDRRLQKGRTTPGRIEEILGRIQPSDDYDDL
                     KGCDLVIEAVFEDREIKAQVTKASDARLSDEAILASNTSGLPISDLAKASSRPTKFIG
                     LHFFSPVERMQMVEIVVGKETSEETLAKAWDYILKIKKAAIVVNDSPGFYTSRVFGTF
                     ISEGNHLLVEGVTPALIENAAKAAGMPMPPLGISDQVGLGTMYKVAKQAERDAAAAGK
                     TWEPSPAQAIIASLIEDHDRWGAHPPPREDGTARTPAGFYDYYEGGKSLWPELGSAMS
                     RWIAAKGEQPSVEIVRRRYTFVQCLEAVRCLEQGVITDVRDGDVGVIMAVGFPAFTGG
                     PFTYIDNYGVAEFVRDSDALSARYGERFDPPKLLRDMAKEGRRFYS"
     gene            complement(9053..>10709)
                     /locus_tag="VFG22_12400"
     CDS             complement(9053..>10709)
                     /locus_tag="VFG22_12400"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6417089.1"
                     /translation="GKLIVDGVVRAAETGTHEDQRATLIWVGVELGLVAVLAGAQKGL
                     STCQQLLRALLGQRVNEMILEKALTLDLAAFEDSELYDRMTRARREASQRPLSLVMRT
                     FQLAQNAFSLIAYGGLLLQLSAWTLAVLVIAAVPSFFVETRFSADAFRLFTWRAPETR
                     KQNYLEVLLAREDFAKEVKLFDLGPVFLARYREIFRDLYTEDRALTLRRGAWGFALGL
                     LSTVAFYGAYAWIAVETIAKRITLGDMTMYLLVFKQGQSALSSMLAAIGGMYEDSLYL
                     SNLYEYLDYETPDEGGTATEGPKPGDGIRFEEVSFTYPGIETPALERVSLHLHPGRKL
                     ALVGENGAGKTTLIKLLTRLYRPTAGRILLDGLELDAWDLDVLRKRVGVIFQDFVRYQ
                     LPAGENVGVGDAKGFSDPERWKVAAEKGMAHEFIERLPSGYETQLGRWFEDGHELSLG
                     QWQKVALSRAFMREEADILVLDEPTASMDAEAEATVFERFGRLAKDKMVILISHRFST
                     VRIADEIIVLERGRVTERGTHETLMHEDGQYARLFTLQAAGYR"
BASE COUNT         2134 a         3200 c         3369 g         2006 t
ORIGIN      
        1 tcgggatgcg cggcacagcg attgggcggg aggccagtcg taggaaaact tggagagccg
       61 gccgagcggg tcccaatcgc gagcacgtgc gtcgcgagcc gccctccgat cggtgcagct
      121 tgaagcgtca gagttcatcc gtggggatct gcataggcgg gctgctcggg atgcgcggca
      181 cagcgattgg gcgggaggct agacgtaggg aaccttgaag agccggccga gcgggtccca
      241 atcgcgagca cgtgcgtcgc gagccgccct ccgatcggta cagatcgaag catcactctc
      301 tgagcgtcag cgtcacagcc aggcgaggag aacgggtatt gaaatcaccg ataccaaggt
      361 gctcacgagc accaggctcg cgatttgctc tgggcttctc ccgtatcgct gggcaaagag
      421 atagttcaag acaccgacag gcatcgaggc ctgcagaacg agaaccttgc gggtgagccc
      481 ctccaagccg aaaagcgccg tgagcagcag cccggcaccg aggccaaggc caaggcgtac
      541 gaacgacaag gtgaccgatc taccgagaga ccgaggatgc atcttcgaaa gcgagacgcc
      601 cagagtgagc agcatcagcg ggatcgcgat gtcgccgagg agcgtggtcg tgcgttcgac
      661 ccaggtcggg atcgcgatgc ccgaggcgat gaccacgacc gtgatcagca ccgcccaggt
      721 cagtggcgag cgaaagaacg gtcgcgccga aaagctgcct gagaacaagg gaccgcccag
      781 gacgacgtga caggtcgaag atacggcgaa atatgccatc ccgagcgcaa gacccgcttc
      841 gccgaaagca aagaggcaaa tcgggaggcc gatgttgccg gagttgctga acgccagcgg
      901 cccgaggaag gtgtgtgcgg gcagccgcat cgccttgagg accaggtacc ctgcgcccgc
      961 gaagatgctc attgctgctg cggccagccc ggccatcctc gcgagctcgg ccggcggcac
     1021 gtcgaggctc gtcaagctgc gaaacacgag gcaggggatg cccacgttca gcaccagccg
     1081 cgaaatcgat acctcgtcga agtgtgcgcc ggatcgggcc cagacgagac ccaccagcgc
     1141 aaccacgaag actggcgcga ccacgctcag aagctcgaga atcacgagcg cctaggcttc
     1201 gaactggccg cgctctttga gcgcttcgag gatggtttcc actgctttgt cgattcgcat
     1261 ttcatcgagc gccacgatga tatccggatc gtcggggggc tcgaagctcg aaaaatccgc
     1321 gtcggggcgt cgttcgcggc agagcgctac gtcggtgttg acgtagacct ggatgaccct
     1381 gtctccgccg attcgctcct tgagcttgtc acggtccgtg cgcgtcggac tcgggaaagc
     1441 acagatgttg atcaggccgg cgtccgtgat cgccttggcg gctgaaatca tcgagcgcag
     1501 atcttctccg actgggctga tgaccgttgc ggtccgacct tcatcgaata gtttccgctc
     1561 gagcgcatat gcaagcgccc agcgcccgga acctggaagg cccgtcagcc aaaccgtcgc
     1621 gccacgttgg ccgaaacgct ccatgcgctc gttcgggctg acgaaggtct tgggctccat
     1681 cgccgattcg gcgtgcagct ccttcatcac ctcgccgatg ttctgctcgc tgcctgcgag
     1741 tgagatcatg ccggctgcaa ccgtgttgtt ggtcaacgaa tcgatgacga tgaaggcgcc
     1801 ggtctcccgg ttgcgctgat aatcgtcgac gtacaacgcc cgatgacagc tcagccgcac
     1861 ctttgcgatg tcgttgagac caaggccgtc agtcggcttc tcctcgagcg tatccatgtc
     1921 gacttcgaag tagattctgt cgatctgcgc gcgtaccgtc tgcgtggtgt gcttgaccaa
     1981 gtaggccttt tcagtgtcga gcggcttttc gtgcatccat acgaggtggg cgtcgaagcg
     2041 tcgggtaacc cgtggaaggt tgccgggttt gacgatcatg tcgcctcggc tgatgtcgat
     2101 ctcgtcttcc agacgaatcg tcaccgactg actcgcaaat gcctcatcga gctcgccgtc
     2161 gtaggtgtcg atcgctttga ctctcgaggt ctttcctgag gggagggcga cgatttcgtc
     2221 cccttttgcg atgacgcccg ccgctacctc gcccgcaaag ccgcggtagt cgagatcggg
     2281 tcgaatgacg tactgcacgg ggtagcggaa gtcctcgaag ttgcgatcgg caaagaccgg
     2341 gaccgtctcc aggtattcga ggacgttggg accgtcgtac cagggtgtgt tctcgctgtc
     2401 gctcaccacg ttgtcaccga cgagggcgct caccgggaag aactggatgt ctttgaaacg
     2461 gagggtcttg ccgaactccg tgaagtcggc actgatctcg tcgaacacct tctcggagta
     2521 gtctctgagg tccatcttgt tcacgcagac cgccaagtgt ggaatgccga gcaaggatgc
     2581 gatgtaggcg tgacggcgcg actgttgaag gaccccatga cgcgcgtcga tcaagattag
     2641 ggcgacgtcg gcagtcgacg cgccggtaac catgttgcgc gtatactgaa tgtgtccggg
     2701 agtgtccgcg atgatgaact tgcgcttgtc ggtcgagaag tagcggtagg ccacgtcgat
     2761 cgtgatgccc tgctcacgct cggccttgag cccatccgtc aagagcgcca ggtccggctc
     2821 gtcgcccttg gaagcggcgc ggacggcggc catctggtct tcgaagacca tgccggtgtc
     2881 gtagaggatg cgtccgatca gcgtagattt gccatcgtcg accgagccga ccgagacgaa
     2941 tcggagcagt tctttttttc gatacttctc gaggtaagcc tcgatgtcat cgcggatcag
     3001 ttctttttcg tcggtcatgc cgttccctag aagagccctg cacggggcct agaagtagcc
     3061 ctcgcgcttc ttgacttcca tcgaaccttc ttcatcgaag tcgatgaggc gcccctgcct
     3121 ttcggagaag cgattgagga gcatttcttg gatgactttg gggaccgtat cggcctcgga
     3181 ttcgaccgcc ccgctcagcg ggtagcagcc tagcgttcgg aaccgcactt tcttcatctg
     3241 tggcttttcg cccggttcga ggcgcatccg gtcatcgtcg acgagtatcc aagtaccgga
     3301 ccgctccacc atcggacgaa cttcggcaaa atagagcggc acgaccggga tattctcgag
     3361 ccagacgtag agccacacgt cgagctcggt ccaattgctc agagggaaga ctcggacgtt
     3421 ttcacccttc ttgatcttcg cgttgaacac gttccagagc tcgggccgtt ggtttttcgg
     3481 atcccactgg tgatggctgt cgcggaagga gaagattcgc tccttggccc gggacttttc
     3541 ctcgtcgcgc cgcgcaccgc cgaaggcgca atcgaactcg tacttcgtca aagcctgctt
     3601 caaggcctcg gtcttcatga tgtcggtgta cttcttgctg ccgaagtcga agggattcac
     3661 tcccatcgcg acaccctctg ggttcgtata cttgatgagc tcgcccttga ggttcgcctc
     3721 gatcttgtcg cggtaccgat acatgtcctg gaacttccag gtcgtgtcga cgtgaagcag
     3781 agggaagggc aggcctcctg gcgcaaaggc cttctccgcg aggcgaagca tgaccgaaga
     3841 gtctttcccg accgaataca tcatgaccgg gttgtcgaac tccgcggccg cttctcggat
     3901 gatttgaatg ctctccgctt cgagctggcg gagttgactt agcttgtgct cgcttgtttg
     3961 caccgcagat tcctagggca gggcttgctg ggacgccagt ctatacgcca gttccggtcg
     4021 agttcaacgc acaatccggt tcatgagagc cccccgcggg gccgcatgct aagagagaga
     4081 ttcgagacat gtcggctatc gtttggatgg atatggaaat gtcggggctc gacccggaga
     4141 ccgaacgcat cctcgagatt gcggttctgg tcaccgatgg cgagctcaac gtggtcgcgg
     4201 agggacccaa cctggttttg catcagccgg atgcggttct cgaagcgatg gatgagtgga
     4261 acacgcgcca ccacggtcag tccgggctca ctcagcgcgt gcgcgagtcg accgtcgatg
     4321 aggcgcaggc ctcggcggca ttgatggatt tcctttccca tcacaccgag aagaacaaag
     4381 cgccgctcgc cggcaactcg atccaccaag acagacggtt cgtggccaaa tatcttcccg
     4441 aggtcgaaga atggctccac taccgaaacg tcgacgtatc gaccatcaaa gagctcagtc
     4501 agcgctggta tcccaagcaa tactcggcgc gcccgtccaa gaaggggagc catcgcgcga
     4561 tggatgacct gatggaatcg atcgaagagc tcaaatacta tcgagcagcc gtgttcctgc
     4621 cgtcggagtc atgaagctcg ccgccctttc attgctggtc tgtatgggct gcgcgggtgc
     4681 catgaagccc gagactcgga cggctgagtc gatcgttcgc agtgagtccg caccggcgtc
     4741 actggtcgat gctgccacag gaaaagagat cgacttagag acgctcctcg ccgccgtccg
     4801 aacgaagcaa gtcctatacg taggggagcg ccacgaccag ccgctcgacc acaacgtgca
     4861 atactcgatc ctccgtcatc tgcatcgaga ggagccctcg ctcgcgatcg gaatggagat
     4921 gtttcaaacg ccgtatcagc agccgctcga cgagtggagg gccggacgaa tcgacgagac
     4981 cgtccttcgc cgcgaaaccg agtacgacaa gcgctggggt ttcgatttca gcatgtacag
     5041 accgatcctc gagtacgcgc ggagcaacgg catcgaagtg gtcgccctca atgcgccgag
     5101 ggagctggcc tatgccgtgg ccaaggatgg ggtcgatggg ctctcgccgg aacaggcggc
     5161 gatgctgccg aagctggacc tcggcaacga gcagcaccgt gcgctcttcg atgaagagtt
     5221 cgaccttgga gaccacgctg cacaagcaga aatcgatctg tattaccagg cgcaggtcgt
     5281 ctgggacgag acgatggcgt cgcgcgtagc agaggcgctc ggccgggcga atggcccctc
     5341 gaagatgatc gtgctcgcgg gtcgggttca tgtgaagcga ggactgggta tccccgatcg
     5401 agcgtccaag aggggcgcag ccccgtacgc cgtcgtgatg ccggtcaccc ggcaggagct
     5461 gaaggccgag ctcaagctcc ccacggaggg tcgttcggca gactttttct gggtggtcga
     5521 gcaatgaatt cctggctgtt tgcgcttcgc gcggagtcaa actagaacac gtttcagttt
     5581 cgggcctccc cggccggagc ggacgcttcc gacgcctcgc gtttggacca gccgaaagga
     5641 aactcgatga cggattgctt catctatgac gcggtgcgta ccccccgcgg caaagggcga
     5701 aagaaagacg gcgcccttgc cgacgcgccg ccgctgcgca tggcaaaaac ggtcctgagg
     5761 gcgctacccg aacgcggcaa cttcgacagc aaggagatcg aagacgtgat cttcggctgt
     5821 gtcgagcccg tcggcgagca gggtgcgaac gtcggccgaa tggctgcgct gatcgctgga
     5881 tacgacgaaa cggttcccgg cgttcagctc aaccgatttt gttcttcagg tctcgaggcc
     5941 gtgaacatcg cggccgcgaa ggtgaaggcc ggtgatgtcg acctcatcgt cgggggtggc
     6001 atggagagca tgagccggat ctcgatgggc gccgcaggag gggcctggtc gaccgacccc
     6061 gcgctcgcct gggggacctc attcgttccg caaggaatcg gggctgactt gatcgcgacg
     6121 aagcacgggt acagccgcga ggacgtcgac tcgttcgccg tcgagagtca gcggcgcgct
     6181 gcagccgcat gggaaggggg ccacttcacg aagtccatag tgcccgtgaa agacgatgtc
     6241 ggcgtcacga tgctcgatcg tgacgagcac atgcgccctg gcacgacgct cgaggacctc
     6301 gcgcagctga aggcgagctt cgaggtgatg ggccggaact acggattcga tgcgattgcc
     6361 attcagaagc accccgaggt cgaacagatt catcatgtgc atcacgccgg caattcgtca
     6421 ggaatcgtcg acggtgccgc tgcggtcttg ctcggaagca aagaggctgg aaagcgtctg
     6481 ggcctgaaac cgagggcgcg aatcgtgagg tccgcaaccg tggggagtga gccctgcatc
     6541 atgctcaccg gccctgcacc cgcatcgcgc aaggccctga ccaaggcagg aatgcgccct
     6601 tcggacatcg acctatggga gatcaacgaa gcgttttcct cggtgtgtct gctcttcatg
     6661 gaggagatgg gcatcgaccg atccgtgacc aacgtgaatg gcggcgcaat cgcgtttggc
     6721 catccgctag gagccaccgg cgcaatgctg cttggaacca tggtcgacga gctcgaacgg
     6781 cgcgatctca ataccgcgct catcacgctc tgcgtcggcg cgggcatggg cacaacaacc
     6841 atcatcgagc gggtgtagga caatggcgat tatcgactat cgagtagacg acggcgttgc
     6901 gatcctcacc tgggatgacc gagaccgtcc gatgaacgtg ctcagcggcg atgcgctggc
     6961 cgagctcggg gcaggtgtcg agaaagccgt ggcggatgac gcggtcaagg gcatcgtgct
     7021 cacctcgggc aaacaaggcg tgttcgtcgc cggcggcgac ctgcaacaga tcgaaacgct
     7081 ggggtctgga cccatcgatg cagcggacct cttgaagcga accggcgccg ttctcgatca
     7141 gcttcggcgc atggagacct gcggaaagcc ggtcgtcgcc gccctcaatg gtgtgaccct
     7201 cggaggaggc tacgagatcg cgctcgcctg tcatcgccgc atcctcgtca atcagcggca
     7261 tgcgcaggtc ggcttgcccg aagcgggcct cggcttgatg cctggtgccg gtggcacgca
     7321 gcgcctgcca cggcttatcg gtcttcaggg tgcgctcggg ctcatcatgc agggaacgca
     7381 gctctcgccc gaccaggcgc tagagaaggg cttggtggac gagctcgtcg agcccccaga
     7441 gctgattccg gcggcaaaga agtggattca cgagaacggc agcgccacgc aaccctggga
     7501 cgaaaagcga taccaggttc cgggtggcgg agcggactcc gtgacattcg cgcaaacgat
     7561 gatgggcgca aatgcgatga cgcacgccaa cacgtacggg aacatcctct cccaggaagc
     7621 gattctgcag tcggtctacg acggcctcgg gctcccgatg gaccgagcgg gcaaggttga
     7681 aacgcgccag ttcatccgcg tcctccaaga tcctaccgcg cgaagcatgt tgaggacctt
     7741 gttcttcagc ttgcaggagt gccagaaagg cgctcgccgt cccgagggcg tacccagatt
     7801 cgacgtgaac aaggtcggta tcctgggggc cggactgatg ggccagggca tcgcgcacgt
     7861 ttcggccaag gtcggaatcg aggtcgtcct gctggaccgc gatctcgcga gtgcacagcg
     7921 gggcaaagac aaggtagcga agactctgga ccgccgtttg caaaaaggcc gcaccacacc
     7981 cggccgcatc gaagagattc tcggtcgcat tcaacccagc gatgactacg acgatttgaa
     8041 gggttgcgac ctggtgatcg aggccgtttt cgaggatcgc gagatcaagg ctcaagtcac
     8101 gaaggcaagt gatgcaagat tgagcgatga agcgatcctc gcgtccaata cttccggtct
     8161 acccatctcg gacttggcga aggcctccag tcgtcccacg aaattcatcg gactccattt
     8221 cttctcgccg gtagagcgaa tgcagatggt cgaaatcgtc gtaggcaagg agacctccga
     8281 ggagacgctg gccaaggcct gggactacat cctgaagatc aagaaggcag ccatcgtcgt
     8341 caacgacagc cccggcttct acacgagccg cgtattcggg accttcattt ccgaggggaa
     8401 ccacctcctg gtggagggtg tcactccagc cctgatcgaa aatgcggcca aggccgcggg
     8461 catgccaatg ccaccacttg gaatctcgga tcaggtgggg ctcggcacca tgtacaaggt
     8521 cgccaagcag gccgaaagag acgcggccgc ggccggaaag acctgggagc cgtctcctgc
     8581 ccaggccatc atcgcatcgc tcatcgagga ccacgatcgt tggggggctc accctccgcc
     8641 cagggaagac ggcacggccc ggacgcccgc aggcttctac gactattacg aaggcgggaa
     8701 gagcctctgg cccgagctcg gctccgcgat gagtcgctgg atcgccgcca agggcgagca
     8761 gccctccgtc gagatcgtcc ggcgccggta caccttcgtg cagtgtctcg aagcggttcg
     8821 ctgcctcgag caaggcgtca tcaccgacgt tcgcgatggt gatgtgggtg tgatcatggc
     8881 cgtgggattc cctgccttca ccggtgggcc gtttacgtac atcgacaact atggcgtggc
     8941 ggagttcgtc cgagactccg atgcgctcag cgctcgctac ggagagcgct tcgacccgcc
     9001 caagctcttg cgagacatgg caaaagaagg acgtcgattc tattcgtagc gactagcggt
     9061 agccggcggc ctggagtgtg aacaggcgcg cgtactgccc atcttcgtgc atgagcgttt
     9121 cgtgagttcc tcgctcggtc acccggccgc gttcgagcac gatgatctcg tctgcaatgc
     9181 gcacggtaga gaaccgatgg gagatcagga tcaccatctt gtccttcgcg agccggccga
     9241 atcgttcgaa aacggtggct tccgcttcgg catccatcga agcggtcggt tcgtcgagga
     9301 ccaggatgtc ggcttcttcg cgcatgaacg cacgtgagag cgctaccttc tgccactgtc
     9361 cgagtgagag ctcgtggccg tcttcgaacc aacggccgag ctgagtttcg taaccgctgg
     9421 gcaaacgctc gatgaactcg tgtgccatgc ccttttcggc ggcgaccttc catcgctccg
     9481 ggtcgctgaa ccccttcgcg tctcccacac ccacgttctc tccagcaggg agctggtagc
     9541 ggacgaaatc ttgaaagatc acgcccacgc gcttccgcag gacgtcgaga tcccaggcat
     9601 cgagctcgag gccgtcgagt aggattcgac ctgcggttgg tcggtagagg cgcgtgagca
     9661 gcttgatcag cgtcgtcttt ccggctccat tctcgcctac caatgcaagc ttgcgtcccg
     9721 gatgcaggtg aagactgact cgctccagtg ccggcgtctc gatgcccggg tacgtgaagc
     9781 tcacctcttc gaatcgaatc ccatcgcccg gcttggggcc ctcggtcgcg gtgccccctt
     9841 cgtcgggcgt ttcgtagtcg agatactcgt agaggttcga taggtagagg ctgtcctcat
     9901 acattccgcc gatggctgcg agcatcgacg acaacgcgga ttggccctgc ttgaacacca
     9961 gcagatacat ggtcatgtcg cccagcgtga tgcgcttcgc gatcgtctcg actgcaatcc
    10021 aagcgtaggc accgtagaac gccacggtgc tcagaagtcc caatgcgaag ccccaggcac
    10081 cgcgacgcaa agtcagcgcc cgatcctccg tatagaggtc gcgaaagatt tctcggtatc
    10141 gcgcgaggaa cacgggtccg aggtcgaaga gcttgacctc cttggcgaag tcttctcgtg
    10201 cgagcaagac ctcgaggtag ttctgctttc gtgtttcagg cgcgcgccaa gtgaacaagc
    10261 gaaatgcgtc cgcggagaag cgtgtctcga cgaagaacga aggaaccgct gcgatcacca
    10321 gcacggccaa ggtccaggcc gacaattgaa gaagcaatcc accgtaggcg attagcgaaa
    10381 acgcgttctg cgcaagctgg aaggttcgca tgaccaggct gagcgggcgt tgtgaagcct
    10441 ccctgcgagc gcgagtcatt cggtcgtaga gctccgagtc ctcgaatgcg gcgaggtcga
    10501 gcgtgagcgc cttctcgagg atcatctcat tgacccgttg accgagcagg gctcgaagca
    10561 gctgctggca ggtgctgagg cccttctgcg cgcccgccaa gaccgcgacc agaccgagct
    10621 cgacgccaac ccagatgagc gttgcgcgct gatcctcgtg ggtgccggtt tctgcggcac
    10681 gaaccacacc atcgacgatc agcttgcca
//
LOCUS       DASRZF010000285         3972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_5022903, whole genome shotgun sequence.
ACCESSION   DASRZF010000285 DASRZF010000000
VERSION     DASRZF010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5022903"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="VFG22_12405"
     CDS             complement(<1..454)
                     /locus_tag="VFG22_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HET6417090.1"
                     /translation="MERVIGIDLGTFNSCVAVVESGTPVVIANRGGYKVTPSMVAVTE
                     GGKRLVGHIAKRQAVTNAENTVFACKRLIGRKWDTPQVKNAIQTCPFHIVEGPHGDCR
                     IKMRDKVYSIPEISAMVLQEMKMVAEDYLGEEVNKVVVTVPAYFNDNQR"
     gene            complement(490..1920)
                     /locus_tag="VFG22_12410"
     CDS             complement(490..1920)
                     /locus_tag="VFG22_12410"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HET6417091.1"
                     /translation="MSEEKIPRPVEGADVRAFDISAQEAFIHSCIDGVLNIEDIADLT
                     VLAPADVFASVERLVSLKLVEWVVPKASSAPPASELLEDVEIDEELQKRIDETYHRAN
                     KMNNYELLGVDLEADRAEIRRAYFALSKVFHPDAYYGKRLGSFKPKMEVLFRKVTDAY
                     EVLGRANKREAYDRYLKQSIAVSTAEQQINRVEERARAMAKALTRTPVVERQAEPPAE
                     PSAASTRRETAAPSKSTPSSEVPGASERERVQRKRELLERRLRGRSRPAPSSSSPVRS
                     MPAPTAKVGKRTALRDLTRSLKQTAVLTGGVDRAEHHLENARVAEAQGDLAKAATALR
                     MAIALDSSNQELKQHYHRVNSHLRVQLVDIHREQARYEENNSMWAAASISWAKVAEAA
                     PEDPIAPRRAAKAVMAAGDDLRKARDFALRSLELAPDSLDARLLLARIYMEAGMDHSA
                     AKELDEAAKLDPGHEMVKNLRKQLAG"
     gene            complement(1990..2901)
                     /gene="argF"
                     /locus_tag="VFG22_12415"
     CDS             complement(1990..2901)
                     /gene="argF"
                     /locus_tag="VFG22_12415"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HET6417092.1"
                     /translation="MTRHFLTISDLSRDELISVLDRADELKALRGTPAHPRPLEGKSV
                     AIVFEKASTRTRLSFEVGIHELGAHPVTLISKDTQLGRGEPIEDTARMLSRYVHAAVY
                     RTFGHDRLEALARHATIPVVNGLSDEYHPVQLLSDMMTIRQNFGPSLDGLRVAWIGDG
                     NNMAHSWIASAAVAGFELVLACPQGYQPDTQVVADARAQGAKIELVEKRDEALDGVSV
                     VTTDVWASMGQEGETDERQKAFAGFIVDDEAMKRAAADAIFLHCLPAHRGEEVSASVI
                     DGPQSRVWDEAENRLHTQKALLEWLIV"
     gene            complement(2898..>3972)
                     /locus_tag="VFG22_12420"
     CDS             complement(2898..>3972)
                     /locus_tag="VFG22_12420"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HET6417093.1"
                     /translation="GGISVLSVGHSHPTLAAAIGEQAARLMHVSNVFYNDRAIELAEA
                     ICARTPFDKVYFCNSGSEANESLLKLARRYHHDRGDVDRVELVSTHRSFHGRTMGSLS
                     LTGQTKYHKGMEPLLQGVRFIYFNDVAALDAAVTDRTAAVIFEPIQAEGGIIVATREF
                     VDAARRICDERGALLFFDEIQTGYGRTGRFLAQEWFETVPDACSLAKGIGGGFPLGAM
                     AVTEKLAHGLPPGSHASTFGGNPLACAAGLAVLRIIDEEGLIENAQRLGEYLAGRLRE
                     LDARLSSTAGTRGLGLLQGLVLADEIDPGATIAAVYAAGLLLTLAGGNVIRISPSLNV
                     TREELDEGLAILEPILADAPRKP"
BASE COUNT          694 a         1258 c         1198 g          822 t
ORIGIN      
        1 ggcgctggtt gtcgttgaag tatgcgggca cggtcacgac gactttgttc acctcttctc
       61 cgaggtagtc ctccgcgacc atcttcattt cttgaagtac catcgcgctg atctcgggaa
      121 tgctgtagac cttgtcgcgc atcttgatgc ggcagtcccc gtgcggccct tcgacgatgt
      181 ggaacggaca ggtctgaatc gcgttcttga cctgaggcgt atcccatttg cgaccaatga
      241 ggcgcttgca ggcaaacacc gtgttctcgg cgttggtcac ggcctgtcgc ttggcgatgt
      301 ggcccaccag acgtttgccg ccttcagtaa cggccaccat cgagggggtg accttgtatc
      361 ccccccgatt cgcgatgacc acgggcgtgc cgctttcgac gacggcaacg cacgagttga
      421 aggttcctag gtcaatccca atgactcttt ccatgcccca cttccaagcg ctcagatgcg
      481 ttagtcacat caccccgcga gctgcttcct aagatttttc accatctcgt gccctgggtc
      541 cagctttgca gcttcgtcga gctcttttgc ggcgctgtga tccatgcccg cttccatgta
      601 gatgcgggcc agcaagaggc gggcatccag ggaatctggc gccaactcga ggctccgaag
      661 cgcgaaatcg cgcgcttttc gcaaatcatc tcctgccgcc atgacggctt tggccgcccg
      721 gcgcggggca atcgggtctt ccggagcggc ctcggcgacc ttcgcccaag aaatgctggc
      781 tgccgcccac atgctgttgt tttcctcgta ccgcgcctgt tctcgatgga tgtcgacgag
      841 ctgaactctt aggtgcgaat tgacccggtg gtagtgttgc ttgagctcct gattgctcga
      901 atcgagagcg atcgccatgc gcagcgcggt tgccgccttg gccaggtcgc cttgggcctc
      961 ggcaacgcgc gcgttctcca aatgatgctc cgcccggtcg accccaccgg taagcacggc
     1021 cgtctgcttg agcgaacgcg tcaaatcgcg gagcgcggtt ctctttccca ctttggcggt
     1081 cggagcgggc atcgagcgta ccgggctgct cgatgagggc gccggtcggg atcgcccacg
     1141 tagcctccgt tcgagaagct cccgcttgcg ttggacgcgc tcacgctccg acgctccggg
     1201 cacctccgat gatggggtgc tcttcgacgg agccgcggtc tccctccgag tgcttgcagc
     1261 cgatggctcg gccggaggct cggcctggcg ctccacgacg ggtgtcctgg tgagcgcctt
     1321 ggccattgcc cgagcgcgct cttcgacgcg attgatctgc tgctctgcgg tggatactgc
     1381 gatgctctgc ttgaggtagc gatcgtaggc ctcgcgcttg ttcgcacgcc cgagcacttc
     1441 gtacgcatcc gtcaccttgc gaaagagcac ttccatcttc ggtttgaacg agccgagtcg
     1501 cttcccgtag tacgcatcgg gatggaacac cttcgagagg gcgaagtacg ctcgccggat
     1561 ctctgcgcgg tccgcttcca agtcgactcc gagaagctcg tagttgttca tcttgttggc
     1621 gcggtggtag gtctcgtcga ttctcttctg cagctcttcg tcgatttcga cgtcttcgag
     1681 cagttcgctc gccgggggcg cgctcgatgc cttggggacg acccactcga ccagcttcag
     1741 cgataccaat cgctcgaccg atgcgaagac atccgccgga gcaagcacgg tgaggtccgc
     1801 aatgtcttcg atgttgagca caccgtcgat gcaggagtgg atgaacgctt cctgtgcgct
     1861 gatgtcgaat gcccttacgt cggcaccctc gaccggtcgt gggatcttct cctccgacac
     1921 agcctggcta gctgcccccc atctggctat tctaacgcgc tagttgctcg gcgagccact
     1981 tcgccgaagc tagacgatca gccactccaa tagagccttc tgggtgtgaa ggcgattctc
     2041 ggcctcatcc cagactcggg actgcggccc gtcgatcacc gaagcactga cttcctcgcc
     2101 gcgatgggct ggtaagcagt ggaggaaaat cgcgtcggcg gcggcacgct tcatggcctc
     2161 gtcatcgact atgaatccgg caaacgcctt ctgccgttcg tcggtttcgc cctcttggcc
     2221 catgctcgcc cagacgtcgg tggtcaccac actaacgccg tcgagtgcct cgtccctctt
     2281 ttcgacgagc tcgatcttcg cgccttgcgc gcgggcatcg gcaacgactt gcgtatcagg
     2341 ctggtacccc tgtgggcagg caagcacgag ctcgaaaccc gccacggcgg ccgaggcgat
     2401 ccacgaatgg gccatgttgt ttccatcgcc aatccaagcc acgcgcagcc cgtcgagcga
     2461 agggccgaag ttttgtcgga tggtcatcat gtcggacaac aactggaccg ggtgatactc
     2521 gtccgagagt ccattgacga cgggaatggt cgcatgacgc gccagggcct ccagccgatc
     2581 gtgaccaaag gttcgataga ccgcggcgtg cacgtatcga ctcaacatgc gcgccgtatc
     2641 ttcgataggc tcaccacgcc cgagctgagt gtccttggat atgagtgtca ctggatgagc
     2701 accaagctcg tggatgccga cttcgaatga gagacgcgtc cgcgtcgacg ccttctcaaa
     2761 aacgatggcg acggatttgc cttcgagcgg cctcggatga gcgggcgtgc cgcgcagggc
     2821 cttcagctca tctgcccgat cgaggacgct gatgagctca tctcggctga ggtccgaaat
     2881 ggtcagaaag tgacgggtca tggctttctc ggagcgtcgg caaggatcgg ttcgagaatc
     2941 gccaaaccct catcgagctc ttctcgagtg acgttgagcg agggcgagat ccgaatcacg
     3001 ttgccgcccg ctagcgtcag gagcagcccc gccgcgtaga cggccgcaat tgtggcgcct
     3061 gggtcgattt cgtctgcgag aaccaaaccc tgaagcaagc cgagaccgcg cgtgcccgcg
     3121 gtgctcgaaa gccgcgcatc cagctctctg agtctgcccg ccagatactc gccgagccgt
     3181 tgagcgttct cgatgagtcc ctcttcgtcg atgatgcgaa ggacggcaag acccgcggcg
     3241 caagcgagtg gatttccgcc gaaggtgctg gcatgactcc cgggaggtag gccgtgtgcg
     3301 agtttctcgg tgaccgccat cgctccgagc ggaaagccac caccgatgcc cttggctagt
     3361 gagcaggcat ccggaacggt ttcgaaccat tcctgcgcga gaaagcgacc cgtgcgaccg
     3421 taacctgttt gaatctcgtc gaagaacagt aacgcgccgc gctcatcgca aatgcggcgc
     3481 gcagcatcga cgaactcccg cgtcgccacg atgatgcccc cttcggcttg aatcggctcg
     3541 aagatgacag ctgccgttcg atccgtgacg gctgcgtcga gtgcagcaac gtcgttgaag
     3601 tagatgaagc gcacgccttg cagcagaggc tccatgccct tgtggtactt ggtctgccca
     3661 gtcaacgaca gcgagcccat cgtccggcca tgaaagctcc ggtgcgtcga gaccagctcg
     3721 acgcggtcta catcgccccg gtcgtgatgg tagcggcgcg cgagcttgag caacgactcg
     3781 ttcgcctcgg agccgctatt gcagaagtag accttgtcga acggagtccg cgcacagatc
     3841 gcttctgcga gctcgatggc ccgatcgttg tagaaaacgt tcgagacgtg catcaggcgc
     3901 gcggcctgtt cgccgatcgc cgcggcgagc gttgggtggc tgtgccccac ggagagcacg
     3961 gaaatcccac cc
//
LOCUS       DASRZF010000286         4800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_212306, whole genome shotgun sequence.
ACCESSION   DASRZF010000286 DASRZF010000000
VERSION     DASRZF010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4800
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212306"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="VFG22_12425"
     CDS             complement(<1..1023)
                     /locus_tag="VFG22_12425"
                     /inference="COORDINATES: protein motif:HMM:NF016158.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HET6417094.1"
                     /translation="MTNPAKRLLILFGNQLFPSAQIEQARPDAVFMAESAHICRRYAA
                     HRHKLVLILSAMRSKADELAEQGQTVFYTQLDEAAHRSWTAMLTQHLDSHDYDELVCF
                     ELESPAMSSKLESLADERGLELRVLPSPMFLTRRQAFEEYRDSHKRLFMADFYREQRK
                     RMAVLIDDEGGPMGGQWSFDEDNRKKLPKSVTPPALPEPEWTDHTRDVVALVDARFPE
                     HPGEAKRFWLPTTEAQAEQALRAFLDERFSRFGDYEDALSSKHPFVFHSVLSPLLNIG
                     LLTPSRVLDAALEKAAEQDIPLNSLEGFVRQIIGWREFIRGVWNTLPPEHWEQNFWNH
                     TGELADA"
     gene            complement(1054..2715)
                     /locus_tag="VFG22_12430"
     CDS             complement(1054..2715)
                     /locus_tag="VFG22_12430"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6417095.1"
                     /translation="MSTSTSPFFTSPEAKAFFQSRVASFGLSIAVAGAIGIAFRIVIG
                     LASGTLLEKLGEPGFWLHASTIVPCMAMWLICRDGPRPLPTVIAAETMGLLFASVGYI
                     GMGMTIPPEVGADVITAFALSFAFFSRAVFVPSTAPRTAMLGLLVGVPLVGMMALHYR
                     DVDPTMWRMVGYDTRGFERSTIIITTALMTTMWWTLTVGLSSIACHVIHRLRIEVSSV
                     RSLGQYQLERKLGEGTMGVVYQATHGMLKRPTAVKLLRPELGGREVHDLFRREVQLTA
                     TLKHPHTITIYDYGRTPEGLFYYAMELLEGASLQDIVEATGPQPVPRVIHILSDVAHA
                     LDEAHSVGLIHRDIKPSNIMLARQGGEYDVVKVVDFGLVKRVSDEEDSEASDARSIKG
                     TPHYLSPEGVNAPQEVDARSDIYALGAVGYCLLTGRPVFEGDTVLEVCMQQVQATPAP
                     MSEARGEPVPKKLEQLILSCLAKNKDDRPQSAQALADALDQLDIPTWTRADAERWWQQ
                     YGPQVEEQRESIVPRITGHTMNPVIDEPWFEGAESAPRSNPTKKT"
     gene            complement(2882..3799)
                     /locus_tag="VFG22_12435"
     CDS             complement(2882..3799)
                     /locus_tag="VFG22_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417096.1"
                     /translation="MKKISCWSAALALSLALMVVGCGESSGGTAGTGGTAGTGGSAGT
                     GGSAGTGGSAGAGGEGGSGGVIGENDIFGIDASTLAVMELNGDSMDGSGNSRNGTLLG
                     GDFVDTEFGQGLRLLGEAPQGINWDAYKDLITHPFSIEMVLVPTDTFDYRRLFRYDDA
                     LDEGWYYGDYLFYSYDNSPIPDVGEPFVADERHYIAIVSREEGAELLIDVYLNGVLVG
                     STPAGYLGSFTDPIANAVFFEDDGGEDLIGVVDALRISSGTRSTDQISAVQSRLETRP
                     DGGPTCDDGLQNGDEEGIDCGGSCPALCI"
     gene            4037..>4800
                     /locus_tag="VFG22_12440"
     CDS             4037..>4800
                     /locus_tag="VFG22_12440"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737012.1"
                     /note="catalyzes the phosphorylation and decarboxylation
                     of oxaloacetate to form phosphoenolpyruvate using GTP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HET6417097.1"
                     /translation="MTVQPTAIAFPTELKSDEAKDFIREVVALCKPAAIHFCDGSEEE
                     YAVLCDRLIEAGTFVKLSEEKRPNSYAAFSDPADVARVEDRTFICSLRKEDAGPTNNW
                     TDPNEMKATLAGLFDGCMAGRTLYVIPFCMGPLGSEISQLGIELSDSAYVAVNMRLMT
                     RMGKAVYQYLEGDRFVRCVHSVGSPLARGAKDVAWPCNNENKYIVHYPEERAIWSFGS
                     GYGGNALLGKKCFALRIASSMARDEGWLAEHMLILGV"
BASE COUNT          860 a         1447 c         1589 g          904 t
ORIGIN      
        1 agcgtctgcg agctcgccgg tgtggttcca gaagttctgc tcccagtgtt cgggcgggag
       61 cgtgttccag acgccacgga tgaactcacg ccagccgatg atctgtcgca cgaagccctc
      121 gagcgaattg agggggatgt cctgctcggc tgccttctcg agggccgcgt cgagcacccg
      181 tgacggagtg aggagaccga tgttgagcag tgggctgagc accgagtgaa agacgaaggg
      241 gtgcttgctg ctgagcgcgt cctcgtagtc gccgaagcgt gaaaaccttt cgtcgagaaa
      301 ggcgcgaaga gcctgctcgg cttgggcttc tgttgtcggg agccagaagc gtttcgcctc
      361 gcccgggtgc tccggaaagc gcgcgtcgac gagtgcgacg acgtcgcggg tgtgatcggt
      421 ccactccggc tcggggagcg cggggggtgt cacgctcttg gggagcttct ttcggttgtc
      481 ctcgtcgaag ctccactggc cgcccatcgg accgccctcg tcgtcgatca aaaccgccat
      541 gcgcttgcgc tgctcgcgat agaaatccgc catgaagagt cgtttgtgac tgtcgcggta
      601 ttcctcgaac gcttggcggc gggtgaggaa catcgggctc ggcagcacac gaagctcaag
      661 gccgcgctcg tcggccagcg attcgagctt cgacgacatc gcgggcgact cgagctcgaa
      721 acagacgagc tcgtcgtagt cgtgggagtc gaggtgctgc gtcagcatgg cagtccagga
      781 gcggtgcgcc gcctcgtcga gctgggtata gaagacggtc tggccttgct cggcgagctc
      841 gtcggccttg gaacgcatgg cgctcagaat cagcacgagc ttgtgacggt gcgcggcgta
      901 gcggcggcag atgtgcgccg actcggccat gaacacggca tccgggcgcg cctgctcgat
      961 ctgggcgctc gggaagagct ggttcccgaa gagaatgagc aggcgtttgg cggggttcgt
     1021 cacggggatc gggggtggcg tcgagtggga ctctcaggtc ttctttgtgg ggttgctacg
     1081 aggcgcgctt tcggcgcctt cgaaccatgg ctcgtcgatg actgggttca tcgtgtggcc
     1141 ggtgattcgc ggcactatgg actcgcgttg ttcttcgact tgagggccgt actgctgcca
     1201 ccatcgttcc gcatcggcgc gggtccacgt tggaatgtcg agctggtcga gtgcgtctgc
     1261 gagcgcttgc gccgattggg gtcggtcgtc tttgtttttc gccaagcagg ataggatgag
     1321 ctgctcgagc ttcttcggga ccggttcgcc gcgcgcttcg ctcatgggag cgggagtggc
     1381 ctgcacttgc tgcatgcaaa cttcgagcac ggtgtcgccc tcgaagacgg ggcggccggt
     1441 gagcaagcaa tagcctactg cgccgagtgc atagatgtcg cttcgcgcgt cgacctcttg
     1501 cggcgcgttc acgccctcgg ggctcaagta gtgtggggtt cctttgatgg aacgggcgtc
     1561 gctcgcctcg gagtcttcct cgtctgaaac gcgcttcacg agtccaaagt cgacgacctt
     1621 gacgacgtcg tattccccac cctgccgcgc cagcatgatg ttgctcggct tgatgtcgcg
     1681 gtggatgagc ccgaccgagt gtgcttcgtc gagcgcatgg gcgacatcgc tcaggatatg
     1741 gatgacgcgt ggaacgggtt ggggcccggt ggcctcgacg atgtcttgca ggcttgcgcc
     1801 ctcgaggagc tccatcgcgt agtagaacaa gccttccgga gtgcggccgt agtcgtagat
     1861 cgtgatggtg tgcggatgct tgagcgttgc cgtcagctgc acttcacgcc gaaacaggtc
     1921 gtgcacctcg cggccaccga gctcggggcg caacaacttc acggccgtgg ggcgcttgag
     1981 catgccatgg gtcgcctgat agacgacgcc catcgtgcct tctccgagct tccgctcgag
     2041 ttggtactga ccgagacttc ggacgctgct cacttcgatc cggaggcgat gaatcacgtg
     2101 acaggcgatg ctcgacaggc cgacggtcag cgtccaccac atcgtcgtca tcagggcggt
     2161 cgtgatgatg atggtgctcc gttcgaaacc acgggtgtcg taaccgacca tgcgccacat
     2221 cgtgggatcc acgtcgcggt agtgcagagc catcatgccg acgagcggga cgccgacgag
     2281 cagacccagc atggcggtgc gcggcgccgt gctcggcacg aagaccgcgc gcgagaagaa
     2341 ggcaaacgag agcgcgaacg ccgtgatgac gtcggcgccg acctcgggcg ggatcgtcat
     2401 ccccatgccg atgtagccca ccgaggcgaa gagcaggccc atcgtctcgg cggcgatcac
     2461 ggtgggaagc ggtcgcggac catcacggca gatgagccac atcgccatgc atggaacgat
     2521 ggtcgacgcg tggagccaga aacccggttc gcccaacttc tcgaggagcg tgccggatgc
     2581 caggccgatc acgatccgaa acgcgatccc aatcgccccc gctaccgcga tgctcaagcc
     2641 aaaggaagcg acgcggctct gaaagaacgc tttcgcctcg ggactcgtga agaaggggct
     2701 ggtgctggtg ctcacgccat tagcttacaa cgggcgggtg acgaataaag gaaaggcgaa
     2761 ttcgggcagg gggcgtgggc ggctgtttgc gccgcgtgtt tcgggactgg cgtccgggcg
     2821 gcccgatcgg tgagccaccc gaactgaatc tccgtgcccg attcgctgag ctcgaaactg
     2881 cctagatgca gagagcgggg cagctaccgc cgcagtcgat gccttcttcg tctccgtttt
     2941 ggagcccgtc atcgcaggtc gggcctccgt ctgggcgcgt ttcgaggcga ctctgtaccg
     3001 ccgagatctg gtccgtcgaa cgggttccgg atgaaatgcg gagcgcgtcg accacgccga
     3061 tgaggtcctc gccgccatca tcttcgaaga agaccgcgtt ggcgatcggg tcggtgaacg
     3121 agcccaaata gccggccgga gtcgacccta cgagcacgcc gttgaggtag acgtcgatca
     3181 gaagctccgc gccttcctca cgggagacga tcgcgatgta gtggcgctcg tcggcgacga
     3241 agggctcacc gacgtccggg attggactgt tatcgtagct gtagaataga tagtcgccgt
     3301 agtaccagcc ctcatcgagc gcgtcgtcgt agcgaaacag acgccgatag tcaaaggtgt
     3361 cggtcgggac gagcaccatt tcgatgctga aggggtgcgt gatgagatct ttgtatgcgt
     3421 cccagttgat gccctgagga gcctcgccga gaaggcgcaa accttgaccg aactcggtgt
     3481 ccacgaagtc tccgccgagg agcgtcccgt tgcggctgtt gccgctgcca tccatcgagt
     3541 cgccgttgag ctccatcacc gcgagagtgc tggcgtcgat cccgaagata tcgttctctc
     3601 cgatcactcc gccgcttccg ccttcgccgc ccgcacctgc gctgccaccc gtgcctgcgc
     3661 tgccacccgt gcctgcgctg ccacccgttc cggcagtccc gcctgttcca gctgtaccgc
     3721 ctgagctctc gccacaaccg acgaccatca gcgctaggct gagagcaagc gcagcactcc
     3781 aacatgaaat cttcttcatc gatatcccat ctacggacga cgtcgcccgg ttgcaaaaca
     3841 tgctagcgga ggagaagagg gagagacaac ttcaagggct cagcggtcgg ttaggtcagc
     3901 caagacgtcg taggcggtgg gcccttcccc cgcttgcacc tcatttcggc acgcgagtgg
     3961 cgatttccat gttgaagaac cggcacggat ggatcagatc tcgactgaga gaacctcaac
     4021 cacgaaagag gcgcgtatga ccgtacagcc gaccgcaatc gcgttcccca ccgagctcaa
     4081 gagcgacgaa gccaaggatt tcatccgaga ggtggttgcg ctttgcaagc ccgcggcgat
     4141 ccatttctgt gacggctcgg aagaggagta tgcggtgctc tgcgaccgcc tcatcgaagc
     4201 gggaaccttc gtcaagctga gcgaggaaaa gcggcccaac tcctacgccg cgttttccga
     4261 cccggcagac gtggctcgcg tcgaggaccg gacgttcatt tgcagcctgc gcaaggagga
     4321 cgccggcccg acgaacaact ggacggaccc gaacgaaatg aaggcgacgc tcgcgggtct
     4381 cttcgacgga tgcatggcgg gccggacgct gtacgtgatt ccgttttgta tggggccgct
     4441 cggctcggag atttcacagc tgggcatcga gctttccgac tcggcctacg tcgcggtgaa
     4501 catgcggctc atgacgcgca tgggcaaagc cgtgtaccag tacctcgagg gggatcgctt
     4561 cgttcgatgc gtgcactccg tcggctcccc gcttgcacgg ggtgcaaagg acgtcgcctg
     4621 gccctgcaac aacgagaaca agtacatcgt ccactatccc gaggagcgag cgatctggtc
     4681 cttcggaagc ggctacggcg gcaacgccct gctcggcaag aagtgcttcg cgctccggat
     4741 cgcctcgtcg atggcgcgcg acgagggctg gctcgccgag cacatgctga tcctcggcgt
//
LOCUS       DASRZF010000287         5762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4001198, whole genome shotgun sequence.
ACCESSION   DASRZF010000287 DASRZF010000000
VERSION     DASRZF010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 5762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5762
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4001198"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="VFG22_12445"
     CDS             complement(<1..992)
                     /locus_tag="VFG22_12445"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HET6417098.1"
                     /translation="MIRAVGIQNPDSDEIESSSESGRPEGTGGLKGKASTGAIWTIAG
                     HGGEQVVRLASNLIVTRLLLAEYFGLMALVSVFLIGLQLFSDIGIGPALVQNKREDPA
                     FVNTVWTIQVFRGLALCAIAFPLGSLFAEFYDEPILTPLIRVSALTAAIRGFTSTSFY
                     TQTRHLRLKGPVVVQFVAQLTGTIVMVGWAWVTRSVWSLVTSGIVAALVMVVLSHVWL
                     PGIRNRFHFERRAARSLFVFGSWIFLSTVATFAANQVDRLIFGKLTTMSMLGVYSIAV
                     MLALAPTQALSTVSNRVLFPLYTRYHYSPADLPSMFRTARWPLLVLGGWAAAGL"
     gene            complement(989..2242)
                     /locus_tag="VFG22_12450"
     CDS             complement(989..2242)
                     /locus_tag="VFG22_12450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6417099.1"
                     /translation="MQAYPLGRQRFAVESAFADHLRLMLKMHKPRFSKITLAGPVMSK
                     ATYQERSAYLAEIDSSQDSIHFVGSYEAGTSDGAFLRGLPKTMSTLRSLVREADLVQT
                     GMNQDLKRPVEFHAAMLASRLGKKTISVTDIDNREAARMFHSSGKWSRRAYLANRFVY
                     DPVRAWQQRQVVDKCSLVLLKGQRLVDDYGQGAEHVKFFQNTAYTAEQVISEEELARR
                     CEEAASSRAPLKLTHFGRLEFYKGMHHAIEALALAGRAEGSAPLAQLTLIGMGSEESS
                     LRRLASTLGVSDAVEFDPPMTYGDAFLSHVRSQDMLIAPSLAPDTPRSAWDAIASGVP
                     ILAYDTTYYADLREKTGCVHTVPWGDVRGLADAIRFFAKDRKSLVRMKQGSRVPALEN
                     VQERWIARRVEWTEALFADNRSKLG"
     gene            complement(2275..3000)
                     /locus_tag="VFG22_12455"
     CDS             complement(2275..3000)
                     /locus_tag="VFG22_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417100.1"
                     /translation="MVGRSTSNSSLFLVLSALLGVSLGIPNAGCGDSGESRTPVAVRV
                     TEYVNLADLEGPMPDAEVCETDTENCVITDEEGRASIELPSDTEVSYTITKEGYGSYL
                     VSDVNTGRLDHRFIMYSDEELARLAPLMGTTYPWTDTGIMILRVYSEPGVMFDLVGQS
                     NQPFYFIDGDTPSTELDATTADGRGGFTDLAEGQYQVEFIDNQVCKAFTAWPGDMANS
                     VKVPVKAGYISWSSMACGQSPTP"
     gene            complement(3117..4094)
                     /locus_tag="VFG22_12460"
     CDS             complement(3117..4094)
                     /locus_tag="VFG22_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417101.1"
                     /translation="MCVPLIHLGYHKTATTWLQNAVFSDETLGFISPWGRQAGIAVDE
                     FVLANPFRFDAVQARSRFQEGLAEARKRELVPVLSNEALCGQPVSGGRFAYGKFVVER
                     LRETFPDGKILIVVREQRAALLSHYREYIANGFYGDLARFIGGPELPPGFAPDCPLDH
                     FEYDALVGHTQSLFGPESVLVLPFEMLKGERESFLAELYRFVGRPVHPVPDRAPERVG
                     VKGLGLAFKQACNRVNFGKSDWARARQPFASRAVSKAASWAERLAPDAWQDAYEERLR
                     HYIEAQIGTRYARNNARLEQLTGLGLSHYGYALPSPETTSRQASARGDY"
     gene            4196..>5762
                     /locus_tag="VFG22_12465"
     CDS             4196..>5762
                     /locus_tag="VFG22_12465"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6417102.1"
                     /translation="MQSVVNPNPDLCVVGAGPAGLAVALRAASGGKSVLLVESGDGSS
                     DLSDLNAGEVVDAASAPDRTDPLLVPGPSLFKPHYLVSGRTRGPGGSSTMWCVRARAG
                     APVTLRIARPERVDFEAQDQFDIPPWPVAFEDIDRYLDEAWEFFGLRESPLEPPDEGC
                     HYPLDPRVFERRYFHFPEASVVHQDRLRDAHQMENIDLRTGWTLLELKTDATKSRVTG
                     LTFVDAKGERFALAPKHVVLATGGVENARILLNAVDEGQLVDPHDTLGRWFMDHPHAR
                     LGVLIPDGDIRETGRFHDFHERDGQTVLGHYSLSHNAVRAQNLLRFSMTLVGTPRVVA
                     TKAAAAGARLAQFRHARLTPLEVAKQLAALARDTRSTLEQFRFKFGPGPRHHTALGGW
                     SDPSTQVTDVGALAVESMVGQRPSRDNRIRLGQRRNRRGKRQARLQWSWSQPEVDSYW
                     RSVNLTREHFARIGVGKFLSAAELGSGTVPRAGTGWHQMGGTRMSLQPSTGVVDADCR
                     HHQTENLFIAGSSV"
BASE COUNT         1143 a         1775 c         1757 g         1087 t
ORIGIN      
        1 atcaagccgg cagcagccca tccgcccaag accagcagag gccagcgggc cgtgcggaac
       61 atgctgggca gatcggcggg agaataatga tagcgggtat aaagaggaaa tagaacacgg
      121 ttcgaaaccg tcgacagcgc ctgtgtcggg gccagggcaa gcatgaccgc gatgctgtag
      181 acacccagca tgctcatcgt cgtgagcttg ccgaagatga ggcggtctac ctggttcgcc
      241 gcgaacgtcg ccacggtgct gaggaaaatc cagctgccga aaacaaagag ggagcgggct
      301 gctcgtcgct cgaagtggaa gcgattgcgg attcccggga gccacacatg actcaacacc
      361 accatcacga gtgctgctac gattccggag gtgactagcg accagacgct gcgcgtaacc
      421 cacgcccagc caaccatgac aatcgtgcca gtgagctggg ccacgaactg gacgacgaca
      481 gggcccttca atcgaaggtg tcgggtttgc gtgtagaagg acgtggatgt gaaccctcgg
      541 atcgccgccg tcagggccga cacccgaatc aaaggagtga gaatcggctc gtcgtaaaat
      601 tccgcaaaca gagatcccag agggaacgca atcgcgcaga gcgcaagtcc acgaaagacc
      661 tgtatcgtcc agaccgtatt gacgaacgcg ggatcttcgc gcttgttctg aacgagggcc
      721 ggcccgatgc cgatgtcgga gaatagctgc aggccgatca ggaaaacgct taccagtgcc
      781 atcaggccga agtactcagc cagcagcaac ctggtcacta tcagattgga agcgagtcga
      841 acgacctgtt cgcccccgtg gcccgcgatg gtccagatgg cgccggtcga tgccttgccc
      901 ttgagcccgc ctgtgccttc cgggcgtcct gactcactcg atgactctat ctcgtccgaa
      961 tccgggtttt gtatgcccac ggctcgaatc accccaactt gcttcgattg tccgcgaaca
     1021 gcgcttcggt ccactcgacc cggcgcgcga tccaccgctc ttgaacgttc tcgagggccg
     1081 gcacacggct gccctgtttc attcgtacca agctcttgcg gtctttggcg aagaatctga
     1141 tcgcatccgc cagcccacgc acgtctcccc acggaaccgt atggacgcag ccggtcttct
     1201 cacgcaggtc tgcgtagtac gtggtgtcgt aggccaggat gggaacccca ctcgcgatcg
     1261 catcccaggc gcttcgaggg gtgtccggcg cgagggatgg tgcgatcaac atgtcctgcg
     1321 aacgcacatg gctcagaaac gcgtctccat aggtcatcgg aggatcgaac tcgacggcat
     1381 cgctcacgcc tagcgtgctt gccagtcgcc gcagggaaga ctcctcgcta cccatgccaa
     1441 tgagcgtgag ctgcgcgagg ggtgcggagc cctccgctct gcccgccaag gcgagggcct
     1501 cgattgcatg gtgcatgccc ttgtagaact ccaggcggcc gaaatgcgtc agcttgagcg
     1561 gtgcgcgcga gctagccgct tcttcgcagc ggcgggcaag ctcctcttcg ctgatgacct
     1621 gctccgccgt ataagcggtg ttctggaaga acttaacatg ctcagcacct tggccatagt
     1681 catcgacgag ccgctgccct ttcaataaga cgagtgaaca cttgtcgacg acctgccgct
     1741 gctgccaggc gcggactgga tcatagacga agcggtttgc gaggtacgcg cggcgagacc
     1801 acttgcccga gctatgaaac atgcgagccg cttctcggtt gtcgatgtct gtaacgctga
     1861 tcgtcttctt gccgagtcgg ctggcaagca tcgccgcatg aaattccacc ggccgcttca
     1921 gatcctggtt catacctgtt tggacgagat cggcctcgcg gaccagagat cgcaacgtgc
     1981 tcatcgtctt cggcaagcca cgaagaaacg cgccgtcact cgtgccagcc tcgtatgaac
     2041 cgacgaagtg aatcgagtcc tgagacgagt cgatttcggc gagatacgca ctgcgctcct
     2101 gatatgtcgc tttggacatg accggcccag ccaacgtgat tttcgagaag cgtggcttgt
     2161 gcatcttcaa catcagacga agatgatcgg caaacgcact ctcgaccgcg aagcgctggc
     2221 gccccagagg atacgcctgc acgggcaaga caaaagcgta gtgaaggtcg cgcttcaggg
     2281 tgtcggagac tgcccgcaag ccatgctcga ccagctgatg tagcctgctt tgacgggaac
     2341 cttgacactg ttagccatgt cgcccggcca ggcagtgaac gccttgcaaa cctggttgtc
     2401 gatgaactcg acctgatatt gtccttctgc cagatcggtg aagccgcccc gcccgtccgc
     2461 tgtggtggca tcgagctcgg tgctcggagt gtctccatcg atgaagtaga atggctgatt
     2521 cgactggccg acgaggtcaa acatcactcc cggctcgctg tacacgcgaa ggatcatgat
     2581 cccagtatcg gtccacggat acgtggttcc catgagcgga gcgagacgcg cgagctcctc
     2641 gtcgctgtac atgatgaagc gatgatccaa gcggccggtg ttcacatccg agaccaggta
     2701 cgagccgtat ccttccttgg tgatcgtgta gctgacctcg gtgtcactcg gaagctcgat
     2761 cgatgccctg ccctcttcgt ccgtgatgac gcagttttcg gtatcggttt cgcatacttc
     2821 cgcatctggc atcgggccct ccaagtcagc caggttgacg tactccgtta ccctgacggc
     2881 caccggtgtc cggctttcgc cggagtcgcc gcagccggcg ttggggatac cgagcgacac
     2941 cccgagcagc gccgaaagaa cgaggaagag acttgaatta ctggtgcttc taccgaccat
     3001 agttgtgaac cctgccgtgt tttatgttga gacgcaagat aacgagaccc gttagaggac
     3061 gccatgatgc cagtttcgag ccgcgaggtt aggcaaatta caggcgggat gggaggtcaa
     3121 tagtccccgc gggccgaagc ctgtctgctt gttgtttcgg gcgagggaag cgcgtagcca
     3181 tagtgactga gccccagccc ggtcagttgc tctagacggg cgttgtttcg tgcataccgc
     3241 gtgccgatct gcgcctcgat gtagtgacgc aggcgctctt cgtaggcgtc ctgccatgcg
     3301 tcgggcgcca gccgctcggc ccagctggca gccttcgaaa cggcccgcga agcaaagggc
     3361 tggcgtgctc gagcccagtc cgacttcccg aagttgacgc gattgcaagc ctgcttaaac
     3421 gcgaggccga gacctttgac accaacacgc tccggagcgc gatcgggcac cgggtgtacg
     3481 ggccgcccga cgaaccgata gagctcggca aggaagctct cgcgctctcc cttgagcatc
     3541 tcgaagggca gcacgagaac gctctcgggg ccgaacagcg actgggtatg gcctaccaag
     3601 gcatcgtact cgaagtgatc aagcggacaa tcgggcgcga acccgggcgg aagctcgggt
     3661 ccgccgatga agcgcgcgag gtcgccataa aacccgttgg cgatgtattc gcggtagtga
     3721 gagaggagcg ctgcacgctg ttctcggacg acgattagga ttttcccgtc gggaaacgtc
     3781 tcacggaggc gttccacgac gaatttgcca taggcgaatc gcccgccgct gacgggctgc
     3841 ccgcaaagtg cctcattgga cagcacaggc acgagctccc tcttgcgcgc ctcggcaaga
     3901 ccctcttgga agcgcgaccg cgcctgcacc gcgtcgaacc ggaacggatt ggccaaaaca
     3961 aactcgtcaa ccgcgattcc cgcctgccgt ccccacggag agatgaaccc gagtgtttcg
     4021 tcgctgaaga ccgcgttctg caaccaggtt gtggcagtct tgtgatagcc gagatggatg
     4081 agtgggacgc acatagccga agtgccgata tgctagcatg cgcttgtgcc ttcctcctgc
     4141 cagctttcag agcgcgattg cgtgcgctcg gtagaaactt cagtttgttt tcgtagtgca
     4201 gagcgtcgtt aacccgaacc cggacctctg tgtggtcgga gctgggcctg cgggcctggc
     4261 cgtggccttg cgcgccgcgt caggcggcaa gagcgttctg ttggtggaga gcggcgacgg
     4321 ttcctcagat ctcagcgacc tgaacgctgg ggaggtcgtc gatgcggcgt ccgcgcccga
     4381 tcgaactgat cctttgctcg tgccgggtcc gagtctattc aagccgcact atttagtctc
     4441 agggcgaact cgtggtccgg gcggctcctc gacgatgtgg tgtgtccgcg cacgagccgg
     4501 tgccccggtc acgcttcgca ttgcgcgccc ggagcgcgtc gacttcgagg cacaagacca
     4561 gttcgacatt ccgccctggc cggtggcgtt cgaagacatc gacaggtacc tcgatgaagc
     4621 ttgggagttc ttcggcctcc gcgagtcacc gctcgagccg cccgacgaag gctgtcacta
     4681 ccctctcgac cctcgggttt tcgagcggag atacttccat tttccggagg caagcgtggt
     4741 ccatcaagat cggcttcgcg acgcgcatca gatggaaaac atcgatctgc gaaccggctg
     4801 gacactactc gagctcaaga cggacgcgac gaagtccaga gtgacaggcc ttacgttcgt
     4861 cgatgcgaaa ggagagcgct ttgcgttggc gcccaagcat gtcgtcctcg cgaccggagg
     4921 agtggagaac gctcgcatct tgttgaacgc cgtggacgaa ggccagctcg tagaccctca
     4981 cgacactctt ggacgatggt tcatggacca cccgcacgct cgccttggcg tactgatccc
     5041 cgacggcgac attcgtgaga ccggccggtt tcacgatttc cacgagcggg atggccagac
     5101 ggtcctcggg cactactcgc tttcccacaa cgcggtacgc gcccagaacc tcctccgctt
     5161 ctcgatgacg ctcgtgggga cgccgcgggt cgtcgctacc aaggccgctg cagcgggcgc
     5221 caggctggcc cagttccggc acgccaggct cacgccgctc gaggtggcga agcaactcgc
     5281 ggcgctcgcc agagacacac gatccacgct cgagcagttt cgcttcaagt tcgggcccgg
     5341 ccctcgccac cataccgctc tcggtggatg gtccgatccg agcacgcagg tcaccgatgt
     5401 cggcgcattg gcggtcgaat cgatggtagg tcaacgcccg tcccgcgata accggatccg
     5461 gctaggtcaa cgccgaaatc gtcgcggcaa gcgacaagca cgccttcagt ggtcgtggag
     5521 ccaaccggag gtcgatagct attggcgctc ggtgaacctc acccgcgagc atttcgcacg
     5581 catcggcgtc ggtaagttct tgagtgccgc cgagctcgga agcggcaccg tgccccgtgc
     5641 ggggaccgga tggcatcaga tgggcggtac gcgcatgtct ctgcagccgt cgactggcgt
     5701 ggtcgacgcc gactgtcgcc accatcaaac cgaaaactta ttcatagccg gaagctccgt
     5761 tt
//
LOCUS       DASRZF010000288         3025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4681970, whole genome shotgun sequence.
ACCESSION   DASRZF010000288 DASRZF010000000
VERSION     DASRZF010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4681970"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..923)
                     /locus_tag="VFG22_12470"
     CDS             complement(114..923)
                     /locus_tag="VFG22_12470"
                     /inference="COORDINATES: protein motif:HMM:NF037012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HET6417103.1"
                     /translation="MFTLRRGKERQHRRRRKQEDWLTFHPGERADPLADGFGTLGIFK
                     EGRLPPRAGIQEPREDAEIITYVREGTLAYEDSIGQSGFIRAGEFQRMTAGRAVRYSE
                     TNASPTEWAHVFQIWLRPSVAGLEPGHEQKHFSFADRHGILCVVASPDARDDSLRIHQ
                     DAFLYSAILDRGGHIAHRLSEGRGAWLHIVRGQVNLGEAVLCTGDGVGITAERVVSMT
                     ARAETEILLVDLDFEATEQLTIEGSTEIANLLVSGTSPPVPADLGYILPRA"
     gene            complement(923..1270)
                     /locus_tag="VFG22_12475"
     CDS             complement(923..1270)
                     /locus_tag="VFG22_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417104.1"
                     /translation="MGRSVETDFYGFKPTDEVGRAVRNWCAAIFSGTQWEKTAQWHVR
                     IDRRSNHEGIATTTQITLRVPERDIHATATHPEVISSVRNAFLSLAGTLRHESPLRLR
                     PESDALKDRAVAG"
     gene            complement(1330..1479)
                     /locus_tag="VFG22_12480"
     CDS             complement(1330..1479)
                     /locus_tag="VFG22_12480"
                     /inference="COORDINATES: protein motif:HMM:NF033488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HET6417105.1"
                     /translation="MLWTIAVVLVVLWALGLVSSYTMGGFIHVLLVVAAIVVLVQIIQ
                     GRRLR"
     gene            complement(1489..1686)
                     /locus_tag="VFG22_12485"
     CDS             complement(1489..1686)
                     /locus_tag="VFG22_12485"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET6417106.1"
                     /translation="MNKDQAQGKWDQVQGRAKKAWAELTDDDFKKAEGSVDKLSGIIQ
                     EKFGDTKKAIQKKLDELHVKK"
     gene            1869..>3025
                     /locus_tag="VFG22_12490"
     CDS             1869..>3025
                     /locus_tag="VFG22_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET6417107.1"
                     /translation="MRAVDLDHKELLELDPEGGIIRFAGQRALLLDAMAMGLLRKSLV
                     DNFGLMAARAVLTQFGFAHGWRMAEAMRAEFTWESDEEWQRAGSRIHTLEGLFRIGAA
                     GDGPLSNEGSMLIASYEAEQHLLHFGRSDMPVCWTICGLISGYLSRTAGEETYVLEDR
                     CMGKGDAACHLFGRTRKQWGDKRAEELRFFEPSHLRESLDVSLHQVTETLKAAESKLR
                     ARRRALVRAAPDVEQPMGIVAKSGAMLKLVDLARRVAKVDSTVLITGESGSGKERIAR
                     LVHEESPRAAGPFIAVNCGAITETLLESELFGHARGAFTGATQDRPGLFEAANTVTLL
                     LDKVGEVSPGMQVKLLRALQEREIRRVGENKSRPFDVRVLAATNRDLTHEVT"
BASE COUNT          553 a          949 c          917 g          606 t
ORIGIN      
        1 aaaatggctg cgattcttat acttgatttc ttcatgattc gattccgttt cttgagggtg
       61 gttgttacac cctcggtgac attgtggagc tgaccgggtc ggttgaaact ttcctatgct
      121 ctcgggagaa tgtagccgag atccgctggc actggcgggc tcgttccaga tacgagcaag
      181 ttcgcgattt cagttgatcc ttcgatcgtc agctgctcgg ttgcttcgaa gtcgaggtcg
      241 acgagtaaga tttcggtctc cgcccgcgcg gtcatcgaga cgacgcgctc ggccgtgatg
      301 ccgacaccgt caccggtaca aagaacggct tcgccgagat tcacctgtcc gcgtacgata
      361 tggagccacg cgcctctccc ctccgacagc cggtgagcga tatgccctcc gcggtcgagg
      421 atggcggagt acaagaacgc atcctggtgg atccggagcg agtcgtctcg cgcgtcaggg
      481 gaagcgacga cacataaaat tccatggcgg tccgcgaagc taaaatgttt ctgctcgtgt
      541 ccgggctcga gccctgccac cgagggacgc agccaaatct gaaaaacgtg cgcccattct
      601 gtcggcgacg cgttcgtctc gctgtagcgt acggcgcgtc cagccgtcat gcgctggaac
      661 tcccccgcac ggatgaaacc cgattgaccg atcgaatcct cgtaggcgag cgtgccttcg
      721 cgaacgtagg tgatgatctc cgcatcctca cgcggttcct ggatcccggc ccgaggtggc
      781 agacggcctt ccttgaagat gccgagggtt ccgaagccgt cggcgagcgg atcagcccgt
      841 tctccggggt ggaaagtgag ccagtcctcc tgcttgcggc ggcgccggtg ctggcgctcc
      901 ttgcctctgc gaagggtgaa catcagcccg cgacggcgcg gtccttcaaa gcgtccgact
      961 cgggacgcaa ccgaagcgga gactcatggc gaagggtccc tgcgagggac agaaacgcgt
     1021 tcctcaccga agagatgacc tctgggtgtg tggcagtcgc gtggatatct cgctctggaa
     1081 ctcgcaacgt gatctgcgtt gtcgtcgcga ttccttcgtg gttcgaacgg cggtcgattc
     1141 gcacatgcca ttgggccgtt ttctcccact gggtcccgct gaagatcgcc gcgcaccaat
     1201 tacggacggc ccttccaacc tcgtcggttg gtttgaaacc gtagaaatca gtctctacgg
     1261 accggcccat gctacttgct cggaaaggcg gccgcgcctt cgcgcggtct atcccctccg
     1321 cctctgagct caacgcagcc ggcgtccctg aatgatctgg acgaggacga cgatggccgc
     1381 caccaccagg aggacgtgaa tgaatccccc cattgtgtac gagctcacca acccaagcgc
     1441 ccaaagcacc accaagacca cggcgattgt ccataacatc gctccgcctc acttcttcac
     1501 gtgaagctcg tcgagcttct tttggatggc tttcttcgtg tctccgaact tctcctggat
     1561 gatcccggaa agcttgtcta cggaaccttc cgccttcttg aagtcgtcgt cggtcagctc
     1621 ggcccaagcc ttcttggctc gtccctgcac ctgatcccac ttcccctgtg cctgatcctt
     1681 attcatgtga ttctcctcgg acgtcgtgtt ctcgatcgag ctcgcgccga catctcggtg
     1741 gttagctaaa gcacgttccg ggccatgatg aggcatgagc aaaatcgagc agttacatcg
     1801 cgagtcgctc gggagctctg gcgcattcag tcattatgat gtaaaaaatc catcaatttg
     1861 aaagaacgat gcgcgccgtt gatctcgacc ataaggagct cctcgagctc gacccggagg
     1921 ggggcatcat ccgcttcgcc ggacagcgtg cgctccttct cgacgcgatg gcgatggggc
     1981 tcctgcggaa gtctctcgtc gataacttcg gcctcatggc cgcccgcgcc gtgctcaccc
     2041 agtttggctt cgcccacggt tggcgcatgg cggaagccat gcgggccgag ttcacttggg
     2101 agagcgacga agagtggcag cgcgccggca gccggattca taccctcgag ggccttttcc
     2161 gcatcggggc tgcaggagac gggccgctct ccaacgaggg ctcgatgctc atcgcgtcgt
     2221 atgaggcgga gcagcatctc ttgcacttcg gccgctccga catgcccgtg tgctggacga
     2281 tttgtggtct catcagcggc tacctcagcc gaacggccgg cgaggagacc tacgtcctcg
     2341 aagatcgctg catgggcaag ggcgatgcgg cttgccatct cttcgggcgc acgcgcaagc
     2401 aatggggtga caaacgcgcc gaggagctgc gcttcttcga gccaagccac ctcagagagt
     2461 ctctcgatgt atcgcttcac caggtcacgg aaacgctgaa ggccgcagag agcaagctcc
     2521 gcgcgcgccg ccgcgctttg gtccgtgccg cacctgatgt cgaacagccg atgggtatcg
     2581 tcgcgaagag cggcgccatg ctaaaactcg tggacctggc gcgacgcgtg gccaaggtcg
     2641 attccaccgt tctcatcacg ggcgagagcg gatcgggaaa ggagcggatc gcgcgcctcg
     2701 ttcacgagga atcgccccgc gccgcggggc cctttatcgc ggtcaactgc ggggccatca
     2761 ccgagacgct cctcgagagc gagctcttcg gacatgcgcg cggcgcgttc actggcgcga
     2821 ctcaggaccg acccgggctg tttgaggcgg ccaacaccgt gacgctgctg ctcgacaaag
     2881 tcggcgaggt ttcgccgggc atgcaggtca agctcctgcg ggcgctccag gagcgggaga
     2941 tccggcgtgt cggggagaac aagagccggc cgttcgatgt gcgcgtgctg gccgccacca
     3001 accgcgacct gacccacgaa gtcac
//
LOCUS       DASRZF010000289         4569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_905504, whole genome shotgun sequence.
ACCESSION   DASRZF010000289 DASRZF010000000
VERSION     DASRZF010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905504"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="VFG22_12495"
     CDS             <1..895
                     /locus_tag="VFG22_12495"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HET6417108.1"
                     /translation="LAAAFVASRLDRTIRSIESVEGLGIPVLGVIPNLGHKGSPAKAD
                     AQSGPKQVGPVAMREELIVVDEPMSPAAETFRMIRTNLTFMSPDDPLRSLVITSALPF
                     EGKTTITSNLAISLAQFGRSVLLVDSDLRRPRLHRVLEVRNDVGLTTLIGGGTPIDSA
                     LHRTKIDGLSILTSGPIPSNPSELLHSAAFGRVKEELLQRFDYVLFDSPPMGAVTDAA
                     ILAQQVDGVLLVVRAGNSTLHAVSGARKQLNSVSAHLLGAILNNADLRIKGYGYGGGA
                     GPYAYRSAGGYAPIREDADAA"
     gene            1018..1437
                     /locus_tag="VFG22_12500"
     CDS             1018..1437
                     /locus_tag="VFG22_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HET6417109.1"
                     /translation="MSELKGSKTHENLAAAFAGESQANRRYLYFAKVADVEGYPEVAG
                     NFRETAEGETGHAHGHLDYLKKVGDPATGLPIGDTVLNLAAAVAGETHEYTDMYPGFA
                     KTARQEGFAEIADWFETLAKAEKSHAGRFQSLLDDIS"
     gene            1456..2844
                     /locus_tag="VFG22_12505"
     CDS             1456..2844
                     /locus_tag="VFG22_12505"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HET6417110.1"
                     /translation="MNDQAKRQISYQPTDGLSYDPSEAKYWDEDALDKEIGRVFEVCN
                     GCRMCFKYCDSFPILFSAIDEDHDGDVGKLTPLDTQRVMDACFQCKLCEEQSPYTPRD
                     GHEFQLDFPKLVHRYDAIRHRKHEQPHKLRLKLLEDPDRAGAAARASFGLANVMNRVG
                     IHRWFMEKLVGIDRRKLLPEFAQTTFEAWAKGAGLTDKGVGGEAVLFQTCYVQGNEPQ
                     IGMDTVEVLQKNGVDVRCEKGLECCGMPAWESGNLELLRKKAHANLDRLMPHVNAGAK
                     VIALNPTCSMMLRREYPELLEGEDRERAKRLAEAVADPSEYLWSLRNEERFNTDFKSS
                     PAGTVAYHAPCHLRTQGVGFKGRDLLRKIPGVAPTTVMECCGHDGTHAMTVEGFEYSI
                     RVGQKAFDEMKSADAEVWATDCPLAAIQFQQHAGVKPMHPMSILARAYREDGFDGPKA
                     LRSGDPQEGGSK"
     gene            2841..3398
                     /locus_tag="VFG22_12510"
     CDS             2841..3398
                     /locus_tag="VFG22_12510"
                     /inference="COORDINATES: protein motif:HMM:NF023432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HET6417111.1"
                     /translation="MKPIERSEILDYVTYGEQRDQIRDSALRAKSVRRILVGEHFTFL
                     FENRETVRYQIQEMMRIEQIVKEDDIQHELDTYNELLHPKGTVGCTLMVGIDDERERD
                     VKLRAWMGLNEHIYARLPNGATVKPTWDPRQVGETRLSSVQYLSFALGDVAPVAVGID
                     MPGVELETELSAAQQDALGQDLGAE"
     gene            3486..4319
                     /gene="htpX"
                     /locus_tag="VFG22_12515"
     CDS             3486..4319
                     /gene="htpX"
                     /locus_tag="VFG22_12515"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF003965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HET6417112.1"
                     /translation="MLRILLFLATNIAVLFMAAIAMAVLTALGVIPPDAPYISMLVWC
                     ALFGFGGSIFSLAISKWVAKRSTGARVIETPSNEIEQWLLSTVGRQAEKAGIGMPEVA
                     IFDSPDPNAFATGMFRNSALVAVSTGLLRLMNKNEVEAVLGHEVSHIANGDMVTLTLI
                     QGVLNTFVLFLARIIASQFDRGRFMIYMALQLVFGIVASIIVAWFSRRREFRADAGGA
                     NLESAGAMASALNRLKSMRDAPSQLPDNLTAFGIRGEGMLKLMATHPPLEERIARLQG
                     R"
     gene            4379..>4569
                     /locus_tag="VFG22_12520"
     CDS             4379..>4569
                     /locus_tag="VFG22_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417113.1"
                     /translation="MGGWRRACWIAAIAIALSGCEEPEQRLERLEITSVDRSCEDGDA
                     CGVVETSCLSKGCECGVAV"
BASE COUNT          936 a         1372 c         1427 g          834 t
ORIGIN      
        1 tctcgcagcc gcattcgtcg caagccggct cgaccgtacg attcgctcca ttgaatcggt
       61 cgagggcctc ggtatcccgg tgctcggggt gatcccgaat ttgggccata agggatcgcc
      121 ggccaaggcc gatgcccagt cgggcccgaa gcaagttggc ccggtggcga tgcgagaaga
      181 gctcatcgtc gtcgacgaac ccatgtctcc ggcggccgag acgtttcgga tgatccgcac
      241 gaatctgacg ttcatgtcac ccgacgatcc actgcggagc ttggtgatca ccagtgcatt
      301 gccgttcgag gggaagacca cgatcacgag caatttggcg atcagcctgg cgcagttcgg
      361 ccgctcggtt ctcctggtcg actccgactt gcggcgaccg aggcttcacc gcgttctcga
      421 agtccgcaac gacgtcgggc tcacgaccct catcggagga ggcactccga tcgactccgc
      481 gctacaccgg acgaagatcg acgggctgag cattctcacc tcggggccca ttccgtcgaa
      541 cccatcggag ttgcttcact ctgcggcctt cggccgtgtc aaagaggagt tgctccagcg
      601 tttcgattac gtccttttcg acagtccgcc catgggggct gtgacggacg cggcgattct
      661 cgcgcagcaa gtggacggcg tgctgctcgt ggttcgagct ggaaacagca cgctccacgc
      721 cgtctccggg gcgcggaagc aactcaacag cgtgtccgct cacttgctcg gtgcaatcct
      781 gaacaacgcc gatctccgga tcaagggata tggctatggc ggcggtgcag gtccatacgc
      841 gtatcgaagc gcgggcggat acgcgcccat ccgagaagat gcggacgccg catagaggcc
      901 gagccacaac tcgttgacga acggggaaaa gcccgggata actcacggtc tcgaccaaca
      961 cgaagcgtgc tctatgttgg tcgggtagac ccatcatcat cggaggaaga acaaaccatg
     1021 tcagagctca agggcagtaa gacccacgaa aacctcgcag ccgcatttgc cggtgagtcc
     1081 caggccaacc gacgctacct ttacttcgcg aaggtcgccg acgtggaagg gtaccccgag
     1141 gttgcgggca actttcgcga gacggccgag ggtgaaaccg gccatgcgca cgggcacctg
     1201 gactacttga agaaggttgg cgacccggcc acgggtctgc cgatcggaga caccgtgctc
     1261 aacctcgccg ccgctgtcgc cggcgagacc cacgagtaca ccgacatgta cccggggttc
     1321 gcgaagaccg cgcgtcaaga aggcttcgcg gaaatcgccg actggttcga gaccttggcg
     1381 aaggccgaga agtcccatgc gggacggttc cagtcgcttc tcgacgacat ttcctaagca
     1441 aggtcagcca cgagagtgaa tgaccaagcc aaacggcaga tatcgtacca gcccacggac
     1501 ggcctgagct acgaccccag cgaagcgaag tactgggacg aagacgcgct cgacaaggag
     1561 atcgggcgcg tcttcgaggt ctgcaatggc tgccgcatgt gcttcaagta ttgcgactcg
     1621 tttccaattc tgttctccgc cattgacgag gaccacgacg gcgacgttgg caagctgacg
     1681 ccactcgaca cgcagcgcgt catggatgct tgtttccaat gcaagctctg tgaggagcaa
     1741 agtccctaca ctccgcgtga tgggcacgag ttccaactgg attttcccaa gctcgtccat
     1801 cgctacgatg cgattcggca ccggaagcac gagcaaccgc acaagctgcg gctcaagctg
     1861 ctcgaagacc cggaccgcgc cggtgccgca gcgcgcgcga gctttggact cgcgaacgtg
     1921 atgaaccgcg tcggaattca ccgctggttc atggagaagc tggtgggcat cgatcgacga
     1981 aagctgcttc ccgagttcgc acagacgacc ttcgaggcct gggcgaaggg cgcgggcttg
     2041 accgacaagg gcgtgggcgg cgaagccgtg ctctttcaaa cctgctacgt gcagggaaac
     2101 gagccgcaaa tcggaatgga caccgtcgag gtgctccaaa agaatggcgt ggacgtccgc
     2161 tgcgagaaag ggctcgagtg ctgcggaatg ccggcctggg aaagcggcaa cctcgaattg
     2221 cttcgaaaga aggcccacgc caacttggat cgcctgatgc cgcacgtgaa cgcaggtgcg
     2281 aaagtgatcg cgctcaaccc cacctgctcg atgatgctga ggcgagagta cccggagcta
     2341 ctcgaaggcg aagaccgcga acgcgcgaag aggctcgccg aagcggtcgc cgacccgagt
     2401 gaatacctct ggtccttgcg caacgaagaa cgattcaaca ccgacttcaa gagctcgccc
     2461 gctgggacgg tcgcttatca cgcgccctgc catttgcgaa ctcagggcgt ggggttcaag
     2521 ggacgtgacc tcctgcgcaa aattcccggc gtggccccca ccaccgtgat ggagtgctgt
     2581 gggcacgatg gaacccatgc gatgaccgtg gaaggcttcg agtactcgat tcgagtggga
     2641 caaaaagcct tcgacgagat gaagagcgca gacgcggaag tttgggcgac cgactgcccc
     2701 ctcgccgcaa ttcagtttca gcagcatgcc ggcgtgaagc cgatgcatcc catgtcgatt
     2761 ctcgcgcgcg cataccgcga ggatggtttc gacgggccaa aagcgttacg ctcgggcgat
     2821 ccgcaggaag gaggttcgaa gtgaagccaa tcgaacgttc cgagattctc gattacgtca
     2881 catacggtga gcagcgtgat cagatccgcg actccgctct tcgcgccaag tccgtgcgtc
     2941 gcattctcgt cggcgagcac ttcacctttc tcttcgagaa tcgcgaaacg gtacgctatc
     3001 agatccaaga aatgatgcgc atcgagcaga tcgtcaaaga agacgacatt cagcacgagc
     3061 tcgacacgta caacgagctg cttcacccga agggaacggt cggatgtaca ctcatggtcg
     3121 ggatcgacga cgagcgcgag cgtgacgtga agctccgagc ctggatgggc ctcaacgagc
     3181 acatctatgc gaggttgccc aatggcgcga cggtgaagcc gacctgggac ccacgtcagg
     3241 tgggcgagac gcgcctttcc tcggtgcagt acctctcgtt cgcgctcggc gatgtcgcgc
     3301 ccgttgcggt cggcatcgac atgccaggcg tcgagctgga aaccgagctt tctgcggccc
     3361 agcaggacgc cctcggccag gacctcggcg ccgaatagtg cttttcggag gaataagaat
     3421 accgcctccg gtgtagttgt cgaaggacgt gacgggtctt aggatggacc cttaggagta
     3481 cgtgaatgct gagaattctg ctgtttctgg ctacgaacat cgccgtcttg ttcatggcgg
     3541 ccatcgccat ggcggttctc accgcgctcg gtgtaatccc gccggacgcg ccgtacatca
     3601 gcatgctcgt ctggtgtgct ctgttcgggt ttggcggctc gatcttctct ttggcgatct
     3661 cgaagtgggt cgcgaaaagg tcgaccggcg cacgcgtgat cgaaaccccg tcgaatgaaa
     3721 tcgagcaatg gctgctcagc accgttggcc gacaggcgga gaaggcggga atcggtatgc
     3781 cggaggtcgc aatcttcgac tctccggacc cgaacgcctt tgcgaccggc atgttccgca
     3841 acagcgcgct cgtcgccgtc agcacggggc tgctgcgtct gatgaacaag aacgaagtcg
     3901 aggcagttct cgggcacgaa gtatcgcata tcgccaatgg cgacatggtc acgctcacgc
     3961 tcattcaagg cgtgctcaac accttcgtcc tctttctggc gcgaatcatc gctagccagt
     4021 tcgatcgggg ccgattcatg atttacatgg cccttcagct cgtcttcggc atcgtcgcta
     4081 gcatcatcgt ggcctggttt tcgaggcgac gggagttccg tgccgacgcc ggtggtgcca
     4141 atctggaaag cgccggggcc atggcctccg ctctcaatcg gctgaagtcg atgcgggatg
     4201 ccccgtctca gctcccggac aacttgaccg cgttcggaat tcgcggagaa ggcatgctga
     4261 agctcatggc gacgcaccct ccgctcgagg agcgtatcgc gcggcttcaa gggcgctgaa
     4321 cctccggtcg atcggtccga ccttgccagg aggcactggc ttcggtagga cgcatgagat
     4381 gggggggtgg agacgagcct gctggatcgc ggcgattgcc atcgcgctct ctggctgtga
     4441 ggaaccggag cagcgactcg agcgcttgga aatcacctcc gtcgaccggt cttgcgagga
     4501 tggagatgcc tgcggagtcg tcgaaacgag ctgcctgtcg aagggctgcg agtgtggcgt
     4561 cgccgtcaa
//
LOCUS       DASRZF010000290        29998 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_906305, whole genome shotgun sequence.
ACCESSION   DASRZF010000290 DASRZF010000000
VERSION     DASRZF010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 29998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29998
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_906305"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VFG22_12525"
     CDS             complement(<1..627)
                     /locus_tag="VFG22_12525"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET6417114.1"
                     /translation="MRPQPIHGLTNAELAERARELLPRGHGVARRIHRRVMLEGRFDP
                     ESWGLGKEACDGWRSRFSFELPQLVTVRSDATDQGAITKMVLRLQDGLEVESVCIPMG
                     RGRYTLCVSSQVGCKMGCTFCETGRMGLLRHLRTDEIIAQLLVARHRLRWPIRNVVFM
                     GMGEALDNAHNVFHALRVLNDEAGLAIGQERLTVCTVGHVEGIRMLDQQ"
     gene            complement(637..1074)
                     /locus_tag="VFG22_12530"
     CDS             complement(637..1074)
                     /locus_tag="VFG22_12530"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET6417115.1"
                     /translation="MRLRDAVTAVRETGDFTAMQQVIPYARFMGITAELVDGDVVGRM
                     QYSDHLVGNASVPALHGGTLGALMESTAIFKLLWRGETTAVPKTINITVEYLRSARAE
                     DVFARAVFTRHGRRVANVRVFAFQDDPERPVAVATAHFLLVQD"
     gene            complement(1071..1553)
                     /locus_tag="VFG22_12535"
     CDS             complement(1071..1553)
                     /locus_tag="VFG22_12535"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET6417116.1"
                     /translation="MSDPFLSQKDDIRRRLEGRYVGAVPHNQALNLRVVDYSPEHLSL
                     ALPYREDLVGNPETGVLHGGCITSLIDATCGGAVIMSLAAPRRIATLDLRIDYVRPAL
                     PHQEVICDAHCYKVTRHVAFVRAAAHHGDAASPIATSSGTFVVFRDDRRQDEDDGDSE
                     "
     gene            complement(1608..2021)
                     /locus_tag="VFG22_12540"
     CDS             complement(1608..2021)
                     /locus_tag="VFG22_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417117.1"
                     /translation="MDVVLAPDIYVNASIALGSPPEQAVRRAFGRPGKPKSSSWVLER
                     VESMLHALPEFKDDAVAAQMKTIGGLVEIVETGSHFIEDWDEALTALAKAAGLHRVLT
                     DHPDLLALKRANGIEFVSSDRWLAEQATPPPPPRV"
     gene            2091..2573
                     /locus_tag="VFG22_12545"
     CDS             2091..2573
                     /locus_tag="VFG22_12545"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HET6417118.1"
                     /translation="MSVRVCFVCLGNICRSPTAEAAMLRLLENEQLTASIDVDSAGTG
                     HWHVGEPPDRRSAMAGKRRGLNIQGRARQVTAEDFHHFDYLVAMDRSNLENLSRLAPG
                     EGARAKVVLFRNFDPSSPPDADVPDPYYGEGDGFEKVLDICEAACRGLLQHVRESHRL
                     "
     gene            2570..3430
                     /locus_tag="VFG22_12550"
     CDS             2570..3430
                     /locus_tag="VFG22_12550"
                     /inference="COORDINATES: protein motif:HMM:NF015818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HET6417119.1"
                     /translation="MRPELRAALEERLGAPIREAKRLAGGDINDAYALTLQNGNRIFA
                     KTHANPPRGMFDAEARGLQWIEETGAIRVPRVLGYSDQRPAYLALELLEPATRCKGFD
                     EILGRALAAMHRSGASSFGLDHDNFIGRLPQSNAPTGDWPTFYWRSRLEPQLRLASDS
                     GLVDSGLRARFDALRHVLPERLGPAEPPSRLHGDLWGGNLHVDESGRPALIDPAAYGG
                     HREVDLAMMRLFGGFGARVFAAYEDAYPLAPGAVERVPLYQLYPLLVHLNLFGSSYLG
                     SLKGALSACV"
     gene            3492..3920
                     /locus_tag="VFG22_12555"
     CDS             3492..3920
                     /locus_tag="VFG22_12555"
                     /inference="COORDINATES: protein motif:HMM:NF014016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HET6417120.1"
                     /translation="MSKTRTDARQSLERRALLLIDHGSRRAQANALLDDVTELVKLRL
                     GTGSIVEPAHMELAKPNIAEGFARCVEQGATRVIVHPFMLAPGRHVSEDLPRLIAEAA
                     EVHEGVAFVMAEPLGSHPGVIDAVVDRCGAVEDEREHGEP"
     gene            complement(3917..4891)
                     /locus_tag="VFG22_12560"
     CDS             complement(3917..4891)
                     /locus_tag="VFG22_12560"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HET6417121.1"
                     /translation="MYRMIVRELGGPGSLQRDELDEVRAKPGQLVIDVKAIGCNFFDI
                     LITEGKYQVRPELPFSPGAEVAGTVLEVGQGVEHFSVGDRVSALLEFGGFASIVAAPQ
                     ERVFHMPVQMSFEEAAALGLVYQTSHVGLVHRANLREGETLLVHAAAGGVGLAAVQIG
                     VALGARVIGTAGSIDKLELVRQHGADEVINYRDEDWVEQVKSLTDGRGADVIYDPVGG
                     DTFNRSTKCVAFEGRILVIGFASGNIPSAKMNHLLVKNYSIIGLHWGLYFDKDPKVLR
                     DAQDAISKLYADGKISPLVSQTYPLVDAKRALDALSSRKTTGKVVLIP"
     gene            4972..5982
                     /locus_tag="VFG22_12565"
     CDS             4972..5982
                     /locus_tag="VFG22_12565"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET6417122.1"
                     /translation="MPGQKRKHSLPAGVLPTKRDEAAEDAGSRIRARVPVEASERPSS
                     PAEPSQTPAERARTLVDELMALAPNEIAPVVRRLLPLGNFALEELARRFPGPLWRPSL
                     ATDSRLPRPEEVSAIAAAIATFEGEAIEYLARLMQDPSAKVRYYAAVICGAYANAELI
                     DPLIQASLDEDRECRRVALHLLSAYQHEPRYGVGLASLRRCASDLECPVPVRRRAISA
                     LTQLRDGASAPLFVDLLGDSDRGIATACRVGLRVLTAHDFGFSRDPWLRWLSERGQEI
                     RIQWLIEGLGDSRANIRLLASRELWHLTRFAQPLSETATRDEFLAVQRNYKRWWSSKH
                     PT"
     gene            complement(5983..6567)
                     /locus_tag="VFG22_12570"
     CDS             complement(5983..6567)
                     /locus_tag="VFG22_12570"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HET6417123.1"
                     /translation="MRRILLWDVMGTLVHDPFFSEMPAFFGMSFESLLEAKHPSAWVQ
                     FELGELSEDEFLRRFFADGRGFDHAGFVAAVRSSYRWLPGLEDLLVELQAKGFEMHAF
                     SNYPIWYRLIEERLGVSRFLDWTFVSCVTGVRKPDTEAYKRVMRELAVPAEQCLFIDD
                     RAGNVEVAREQGIDALVFEGVGRLRTSLGNAGFL"
     gene            complement(6572..8794)
                     /locus_tag="VFG22_12575"
     CDS             complement(6572..8794)
                     /locus_tag="VFG22_12575"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET6417124.1"
                     /translation="MGVSLAIAMASARSGSTSLEYTHSIAWLLAALVMVDLVLPRQTG
                     SVVRSAVIAGFALAAVVGGALARDNLLPAPTFGVVVVSAILATGAFHQVLLVGRGHGV
                     EGVLSGVALVSLGVALAYAWFGPFTGLLGTGVEFAVAGILWLGHLAWLDPGWRSLRRL
                     GVPLVVASASCFILTYALVPDAPLERSELAALGLASGVLWWGVFSLARRLSDRAVWST
                     SGRLADAAQVARHNLLGAATLEEIASAALVPLGKALEASDGLPEIFTLEPPLRMRMDV
                     GERANVKSGDAPAAIAKALFVDELQGVLDLPSLRSRVVREPMVRTLVDSMQDQGIGMV
                     VPCQHLEHIEGLLVLPGSDRSEPAMRIEIEELRRLSETLGGSLAAALAHRRADTRIHQ
                     ISAERRAAEDRITALQAEVQQLRQQCDVLGRGLAEDQTLHVAYSQSMRRVQTRAIELA
                     PLTKPILLVADAGSPVLPVSRFIHDRGPRWRAPFIVADCSAAPPEQVMRLLFGSESEH
                     RAGWFQSAIGGTLLLRDIPALGKQAQARLASSLIEHNQTENEGEQTPEVSPRIIATSR
                     TTLIELQRRGALDPDLARCFSGPGLKIPRLRDRREDVPSLALLAIDRACRVLAREPVG
                     IDQAAMVALVDHEWPGDVAELELVIELAVGRTSSKTISITDLPPLAWPGAAREGSLDG
                     TYLEVERRLLEYALSRSGGNKSEAARMLGLKRTTFLDKLRRHGLEQRPQEDLGGSAIG
                     "
     gene            complement(9023..10993)
                     /locus_tag="VFG22_12580"
     CDS             complement(9023..10993)
                     /locus_tag="VFG22_12580"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417125.1"
                     /translation="MSVLASLLVRDQLIAVDRVQAAIQEQVMRGGNLDTVLLELGLLR
                     ENEMNAYCAAVFGLLPATRNEVMQSPISSIRVLPREFAVRHRLIPVARTEDAVVLAVD
                     RPLPDATLREISFELGIDPVQRVVCTVRVEAGLARHYDIEMTARMRHLCNQLDAADAG
                     SIPYVAPFEGVGKRSSLPPKKDVSGSYPRGDSSIPAPVGMADTEPPPDILPQIGSSVP
                     AGTFRNSDPGAPPRKRSDPKSGFIDATRYSLPPRPPEVLGQPFARKRASRPAQSPNQD
                     PFGGWHPAAAPRSTPKRRSAPSDPRRDNDGSAISHDVVSLGPHGPSSAPPNADSEPAG
                     RRWAPAPPPSGSNGSLPSVIVDVGDDVEQLVAALRDAGPEDQMLILPLLAVGEVALPA
                     LAREFPGNLWFDRNEPHARIPAGRDISPIARTFVAFGRRSVPYLIPLLDHEDPDIRYY
                     ATVLAAEFVHPDLVRPIGRRLFDSDPGARNSAYRALSVLYACELEFTQLIERLRASAR
                     DDRELRPQVTSIEALGKLRDADSFDFLISQLESPEIEIVKAAHASLVRLSCQDFGTHR
                     KKWASWLRKHGDRHRVEWLIDGLMHSDERLRRRAGDELKHLTQEYFGYEPGLSKKQRE
                     AIQQKYRLWWQRVGFRMFVEPEPNDSGHAHGQ"
     gene            complement(11042..11791)
                     /locus_tag="VFG22_12585"
     CDS             complement(11042..11791)
                     /locus_tag="VFG22_12585"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HET6417126.1"
                     /translation="MRLRAQRGAAMSVRRLYVPDLPERGGQVTLDESTSRHVRVLRLR
                     VGEELLLFDGRGRAAPARLESVGEQIVCKAEPPSASEERRVRLVLMLAVPKGSKLDDC
                     VRMATELGVDEVALMLTERTVPRWDPERERSRIDRLTRIASEAAAQCERNDVPIVRAP
                     RPYTDWLDEMPTGACGVLFGARAKGALKLEFIPQQLWCALGPEGGFTDGEINRFERAG
                     FSVASLGARVLRVETAVPAALAIVQDRLAAC"
     gene            complement(11758..12618)
                     /locus_tag="VFG22_12590"
     CDS             complement(11758..12618)
                     /locus_tag="VFG22_12590"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HET6417127.1"
                     /translation="MSDSSHHPFVTVRVEPERIELAELRLWELGATGLEERDETTLVR
                     EPAPALPMIIASFPDEASARYALQELRDEYDAKITYEAQQDWAIEWRRGFSAQRIGKR
                     LLLHPSWEKVESKPGDVVLSIDPENAFGSGDHETTRLVLQILDERVAGGERVLDVGCG
                     SGVLSIAALRLGAASAVAIDIEEDAVVVALRNAALNAVAPRIEVSTRPLAEITGLYEI
                     VLANIETRILVHMPEQLEARLAPGGILVLSGILRVERDELLAAYASMHLESVIEEGEW
                     CACVLSAGLR"
     gene            complement(12618..12854)
                     /locus_tag="VFG22_12595"
     CDS             complement(12618..12854)
                     /locus_tag="VFG22_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417128.1"
                     /translation="MELWLLALWALSGAALLFTHLLMAWRVLAGPLAAQWRYLGFLVP
                     VFTPIVAWRGGNRLGPITWVLFLVIYVSARMIEI"
     gene            12953..14260
                     /locus_tag="VFG22_12600"
     CDS             12953..14260
                     /locus_tag="VFG22_12600"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HET6417129.1"
                     /translation="MSSRTIRIGLVGCGVVGTGVLNLLRTNAATIQKRLGAALAVEQI
                     AVKNLKRKRSDLVPREILTDDPLAVARNPKVDIVVEVMGGIEPAEQVVREALEQGKQV
                     VTANKALLAEKGDAIFDLAKQKGLDVSFEAAVAGGIPIIRTLEESLASDEIVSVQGII
                     NGTSNYILTEMAERGLDFDVALKSAQAAGYAEADPTLDVNGGDAAHKLAILATLAFGV
                     RVHPAHIATEGIDGVDASDIEYADGFDYAIRPVAVASRSAGNVLDLRVHPALVPYDNP
                     LANISGALNAVSVRGEQVGTCLLSGPGAGAGPTAMSVVSDVIDVARNILKNASGRVPS
                     FIHDGDAEPLVQDPNDLRASYYLRFTVRDEPGVLSKITGKLSDFGISIEKMFQNVQAG
                     AEGAATVVIVSHKAREGDVHKALSELDPLPHTLAPARLLRILG"
     gene            14407..16350
                     /locus_tag="VFG22_12605"
     CDS             14407..16350
                     /locus_tag="VFG22_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417130.1"
                     /translation="MLGLLAVASTTPADCEVDQAAVSACMSPVPDVSASRGWQSRTAP
                     QAASSELFFEVMARPTAANLNGLVTIGSGDINDFSDAAILVRFADNGEIDVRNGSQYD
                     SDRSIAYVPGVWYTIYITADVVAETYKVEVGTCGGSRETLVSRASFRPDAPSGEPLSN
                     WAAWSSQDASLEVSTPAWSASGSCAPATCESLAHECGQPSDGCGGSLDCGACASGNVC
                     RSGVCVDESVPAPTPTPTPTPTPTPTPSTGFPTYATTGITEEWCPRWDSLKRVTSGRT
                     ITAANTVIEYERINGKLNIDANNVTVRCVEIVTGSDVNTLGCSNNGNPTYGCDEGFLI
                     EDAKVYTTGGNNNAPFMFVTDEASGRRVQVYGGCDEMKMDGGYLRDSLIDGNTNYSCN
                     LSGPHTDVWQSSGSNGPIWMVNNTMIGPYKSQNGTVFIKTDSGQNHDYTIDGCQLSGG
                     SFTVWARNGGNGIPFDWRFTNNTIIENSYTYGAFDIEGNGPSQCIEWSGNVWDDKTPA
                     TGGPSNVKPIGGCSGVTPIPTWSVGGLALTGTSCTRGGAACDNVDLRASASGSQSGGS
                     PRWRFNCGGSAGNTGGNHPEGADLWYDYTPCNGQRSCTMTDVCDYSGEASGTYRVRIY
                     SESGPGSRTRISDTSEASFVVNN"
     gene            complement(16444..17298)
                     /locus_tag="VFG22_12610"
     CDS             complement(16444..17298)
                     /locus_tag="VFG22_12610"
                     /inference="COORDINATES: protein motif:HMM:NF018677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrase ferredoxin"
                     /protein_id="HET6417131.1"
                     /translation="MNLNACSVASLEAGETLAATAVESVDRWLLLEVAEPWAPKALQT
                     EALPATVRAKLGDWLGTPRSRLQLIRRPGRSGKRPLIMQVRSGADRQESSKLQLDQYE
                     DLLDVDLDALPAEALAPICLVCVHGRRDRCCALHGSSVFRAIQGQGVDTWQTSHLGGH
                     RFAACAVWLPDGLMYGRLRAEHAPALVAARQAGELGNIDLFRGRCAYDRPAQAAEIFL
                     RRKLSAQDASALELIGSDSEDESTWVARFRTTSGETSVRVRLESTGIDRPASCGGEPE
                     PVTRFVEL"
     gene            complement(17301..17618)
                     /locus_tag="VFG22_12615"
     CDS             complement(17301..17618)
                     /locus_tag="VFG22_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417132.1"
                     /translation="MKLSSDEERWAVWMVQARRFAERENFTDAVARMRLVRDAVGKAL
                     AEATDSRKRERLESELARADEQLASLESKYLAWRSEIAARRQTTIDQAEEEMARPLPV
                     QVD"
     gene            complement(17631..19205)
                     /locus_tag="VFG22_12620"
     CDS             complement(17631..19205)
                     /locus_tag="VFG22_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417133.1"
                     /translation="MTGPAPLLWLAWLCAGATIVYVVGAPFWVADYPMMTDFPFHTAS
                     SSVFRHYADPGWHFHEQFVFQMFAVPYVTLYAVSAIFMLLLPPVVATKAAAALLLALL
                     PLGLMVLCWGLRKSPLLGLWGLVAVWTVPSHWGFVGFLGALGLFAMAIGLALRVVDRP
                     SIKVQGALIAVLILLFFTHVYRFPFALLMLVVVGAVMQGRAESIRGLVIPVAVAGTLF
                     TTWWLTRVDLLMPAMSWTWPPAWNRMSEISSYTTDIFTGDEDTAMFRRTGLLFMVTAV
                     GLLAIALLRLRSWPRGGWVVPAHAVVGVAILVLLGLYLTLPMELGIWWWVFPREITAA
                     LFLLPALLPNLPRKTWAHLGFVIWTAVGIAPLGEFVAEAHREFSTSTVHFREIVRELP
                     KAPKLLYLVYDHQGSRARNSPYIHLPAYVQAERGGWLSFHFAQLGTFPFRYRDPSDPN
                     AVVPPKTPVRWEWTPEQFQLEEHGDFFDWFLVRRVSSPDFLFVSDPSIQRVAHFENWW
                     LYHRPRPSTGPPEETP"
     gene            complement(19266..20135)
                     /gene="atpG"
                     /locus_tag="VFG22_12625"
     CDS             complement(19266..20135)
                     /gene="atpG"
                     /locus_tag="VFG22_12625"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HET6417134.1"
                     /translation="MANLKDIRKRIGSVKSTQKITRAMKLVAGARLRKAQENIERLRP
                     YAVKTHEVLSGVSARVGADEDVHPLLARREPKNVMLVVLTSDRGLAGAFNSSICKAAY
                     GRWKQLEAQGSEVCFGIIGRKGRDYFTRRNADIRHNFLGVFEDLSSDKAGEIGRYIVE
                     DYTSLNLDGCYLVYNEFKSAITQQVVIEPFLPIEPLELADDRMGDFIYEPNQTALLDR
                     LLPMHVEVQIYRALLESVASEHGARMTAMDSATNNAADMIDRLTLQYNRARQAAITKE
                     LMEIIGGAEALKG"
     gene            complement(20142..21794)
                     /gene="atpA"
                     /locus_tag="VFG22_12630"
     CDS             complement(20142..21794)
                     /gene="atpA"
                     /locus_tag="VFG22_12630"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HET6417135.1"
                     /translation="MQLRAEEISNIIKQQIASYEKSVDVVETGTVLSVGDGIARLYGL
                     ESAMAGELLEFPGGVYGMVLNLEEDNVGCVIFGSDVGINEGDTVKRTGRIVEVPVGPA
                     LVGRVVNALGQPIDGKGPIESKLNRRVEIKAPGIVARQPVSEPLQTGLKAIDSMIPIG
                     RGQRELIIGDRQTGKTAVALDTIINQKGKGVFCVYVAIGQKQSTVAQVVDKLTQFGAM
                     EYTTVVLAGASESAPLQFIAPYTGVTMGEYFRDSGQHALCIYDDLSKQAVAYRQLSLL
                     LRRPPGREAYPGDVFYVHSRLLERAAKMAEDWVVVKEDQEPSRLGNKVYTGPKSKHEA
                     QAAAKEKGAGFVAKKLPDSGGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLESDL
                     FNSGVRPAINVGISVSRVGGNAQIKAMKQVAGTLRLELAQFREMAAFAQFASDLDAAT
                     RQQLSRGERLVEVLKQGQFVPWSVEEQVVVIYAGTHGFLDVLPPQSIGRYQQELISYL
                     KAERAAILSDIVSKGKIDDTLESQLQDALKAFAEIFEPVQDN"
     gene            complement(21821..22369)
                     /gene="atpH"
                     /locus_tag="VFG22_12635"
     CDS             complement(21821..22369)
                     /gene="atpH"
                     /locus_tag="VFG22_12635"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HET6417136.1"
                     /translation="MSALGRRYAKALLDLAREQGELDTTLKDVGALSDAWKASKELRE
                     IVANPVIPRPALKAAVDAVMQKLGCSKLVRNTVNLLADKGRLGHLDDVLQALEQLAEA
                     ETGRVRVEVVSAKPLSDAYYARLVEKLQRVSDRKVVLVKREDPSLIGGVVTRVGDQVF
                     DGSLSNRLSELRETLLANGETP"
     gene            complement(22366..23022)
                     /locus_tag="VFG22_12640"
     CDS             complement(22366..23022)
                     /locus_tag="VFG22_12640"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HET6417137.1"
                     /translation="MRLLGAMFLMLALTSPSLVSAEAAYDESEAHQAAEEQSGHDAHG
                     DHGELTVTGLLKDRVFQGTVINFLVLVGLLTWVIRKKGNPALAARRAEVENELAEAQR
                     LRAEAEKRHRETATRLEKLDQEMAQIRHDMVKAGEAERDRIVEQAEEKAARMRKDTSF
                     LIEQQIKQLRKELTHQAANAAVFAAQELLQEKTSASDQDQLAEAYLTRLGEVLEERKS
                     "
     gene            complement(23022..23489)
                     /locus_tag="VFG22_12645"
     CDS             complement(23022..23489)
                     /locus_tag="VFG22_12645"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HET6417138.1"
                     /translation="MNVLFTALLSEGSIIDLDGTIWIQLALFAVAFFVFRPLIFRPMV
                     ALFEARENAIEGSKLEALRLQDEASAESEEFEVEMRRIRLQAGEERDRLRNEGKRLER
                     TVLDRVREETGKQLAEADAKLAKEASRLRAEIDQSVPVLARQIASKMLNREVQ"
     gene            complement(23671..24270)
                     /locus_tag="VFG22_12650"
     CDS             complement(23671..24270)
                     /locus_tag="VFG22_12650"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET6417139.1"
                     /translation="MNKKSLIGIAAATWIFAVAASPALAAEEPNENAGILTAQILEEG
                     SNIAWGQAVLVVDRPIDEVLPIVQDYANYADFMPNFTKSRVLSQRGSRAMVYMEVSVA
                     KGAFTLWGQLNLAERPQEGDSRVVEAYLMEGNVDAFSARFELTPVDAGTRTRVDFKIY
                     VDPDIPLPSSVFSRENERAAGKTLRALRTRVFEGPRGST"
     gene            24561..25301
                     /locus_tag="VFG22_12655"
     CDS             24561..25301
                     /locus_tag="VFG22_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417140.1"
                     /translation="MQPETYERPVLTVLGGGGPRFQRIKLEVGDGAGGIEPRDFQTGA
                     YFDFGWFVLARPAARKLSRPSVQAIVLQLEGGAGVGLTVEPSGSGISLQTRAWRLLGQ
                     FGYLYPMGRVQVGGLLGVGADLLKIDLNSVLPSSRIIYFRLGPAVTYQLLKQLLDLRV
                     DLGLRAPFVLDGLADAFGNDARGIGMDAALMFNGRLQNGLSYAVRLIWEHYWLRFTGT
                     SAGVPASGEGAEGGDNAITLHFLVGWSL"
     gene            complement(25274..25696)
                     /locus_tag="VFG22_12660"
     CDS             complement(25274..25696)
                     /locus_tag="VFG22_12660"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HET6417141.1"
                     /translation="MQDQDTVIQALLGAGTRYEGKLFFEGRARIDGEFEGEVFSEGLL
                     VVGQQARVKGSIRVHSLIVSGGQIEGDIHATALVELHAPSRIKGDIRTQVLFLEKGVV
                     FDGSCVMGEVIELSERPSTPRAADDTSPDGYKDQPTKK"
     gene            25889..27325
                     /gene="atpD"
                     /locus_tag="VFG22_12665"
     CDS             25889..27325
                     /gene="atpD"
                     /locus_tag="VFG22_12665"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HET6417142.1"
                     /translation="MAELQNGQVTQVIGPVVDVRFPQGSLPEILTAIQVTNPAINDKD
                     WNLTLEVAQHLGENTVRAVAMDSTDGLVRGMAVKNTAAPISMPVGKECLGRILNVVGA
                     PVDELGPVNATKFSPIHKAPPSFVDQSTEIEIFETGIKVIDLLAPYRKGGKIGLFGGA
                     GVGKTVLIMELINNVAKAHGGVSCFAGVGERTREGNDLLLEMSESKLETGETVLSKTA
                     LIYGQMNEPPGARQRVALSALTVAEHFRDEEGQDVLLFVDNIFRFTQAGSEVSALLGR
                     IPSAVGYQPTLATEMGQLQERITSTNKGSITSVQAIYVPADDLTDPAPATTFAHLDAT
                     TVLSRSIAELGIYPAVDPLDSSSTMLDPAVIGDRHYNVARGVQETLQKYKDLQDIIAI
                     LGMDELSEDDRMVVDRARKIQRFLSQPFFVAEQFTGLKGAYVPLEESIAGFEEILDGK
                     LDHIPEQDFYLKGNIDQVKEAFSKRAKD"
     gene            27338..27766
                     /gene="atpC"
                     /locus_tag="VFG22_12670"
     CDS             27338..27766
                     /gene="atpC"
                     /locus_tag="VFG22_12670"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HET6417143.1"
                     /translation="MANSEYLQLDVVTPSGSVLSARVDSVQAPSVQGEFGVLPNHLPL
                     LAALKCGLLVWEVGGKRKIAAIGPGFVEGGPDKVLLLTDLFASPEQIKPDAVREELAK
                     AEEALKAFDELYEGPEYNELQRDVDWAHARLEAYAAARAV"
     gene            complement(27763..28500)
                     /gene="rnc"
                     /locus_tag="VFG22_12675"
     CDS             complement(27763..28500)
                     /gene="rnc"
                     /locus_tag="VFG22_12675"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HET6417144.1"
                     /translation="MSNRGEPLDDASSRLCQELGYSFANTGLLLDALTHRSFKNERPD
                     LAATDNERLEFLGDAVIGLLVASLLYLQFPDADEGELTRRRADLVSEKGLSEAAEAID
                     LGDAMRLGKGEQKSGGRSKPRLLASALEALVGAVYSDGGADAAFSVARRIFEPRLHTR
                     APGHRDAKSRAQEWAQANLGGTPIYRLLGTDGPDHEREFTVAIELHDEEVATGAGRSK
                     IEAEQDAASAALAIWVELAALAETAAD"
     gene            28568..29113
                     /locus_tag="VFG22_12680"
     CDS             28568..29113
                     /locus_tag="VFG22_12680"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6417145.1"
                     /translation="MNFPPEELETRRLLLRRPTLEDAEAAFASYASDAKITRYLTWSA
                     HTSPAQTREFFQQVIDAWDLRMGHRMWLIERKEDAELMGTIGCTVHFHRAEIGFALGS
                     RFWGNGYMPEALARVCEAAFADPRIERVQALCDEENTQSSRVLEKVGMQYEGRLRRYG
                     HHPNRSDVPRDCLMYALIRGD"
     gene            29148..>29998
                     /locus_tag="VFG22_12685"
     CDS             29148..>29998
                     /locus_tag="VFG22_12685"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET6417146.1"
                     /translation="MRLLTTVSDQNQAKVLGDALYADGVVTTLKETRDGSFAVWVHDE
                     EKMEQARAFLNGFDPGASRFAEMAKKARAKRKQEARSDEQLRVRTEKVRRQIEAKQNM
                     RIGPVTTGLIAICVLVFLLTNQGNDLGMLSYFTFVPLVPAGNRFVFGDVSAIWQGQPW
                     RLFTPMFIHFGFMHILFNMWWLKDLGTAIERVFSSRYLLTFVLLTAAFSHVLEYAVSG
                     PTRFGGMSGVVYALFAFMWIRGRFDPSFPYRMPQQLVTFMLIWLGLGFTGWVGPIANW
                     VHTGGLIA"
BASE COUNT         5724 a         9362 c         9464 g         5448 t
ORIGIN      
        1 ttgttggtcg agcatccgaa ttccctcgac atgtcctacg gtgcagacgg tcaggcgttc
       61 ttgcccaatc gcgagaccgg cctcgtcgtt cagcacccgc aacgcgtgga atacgttgtg
      121 cgcgttgtcg agggcctccc ccatgcccat gaatacgaca ttgcgaatcg gccaccgaag
      181 acggtgccgg gccacaagca gctgagcgat gatttcgtcg gtacggagat ggcgaagcaa
      241 acccatgcgt ccggtttcgc agaaggtgca tcccatcttg caacccacct ggctcgacac
      301 acacaaggtg tacctgccac gccccattgg aatgcaaacg gactccacct cgaggccgtc
      361 ctgcaagcgc aagaccatct tggtaatcgc gccttggtcg gtcgcgtcgc ttctcacggt
      421 gacgagctgg ggaagctcga acgaaaatcg cgaacgccac ccgtcgcagg cttccttacc
      481 gaggccccag ctctccgggt cgaagcgacc ttcgagcatg acgcgccgat gaatgcgccg
      541 ggccacaccg tgcccgcgag gcaatagctc cctagcgcgt tccgctagct cggcattggt
      601 caggccgtga atcggctgtg gacgcattcg aacgcctcag tcttgaacca gtagaaagtg
      661 tgccgtggcg acggccaccg gacgttcggg atcgtcttga aatgcgaaaa cacggacgtt
      721 tgcgacgcga cgcccatgcc tcgtgaacac ggcgcgggca aatacgtctt cggcacgcgc
      781 acttcgaagg tattcgacgg tgatgttgat cgtctttgga actgcggtgg tctctccgcg
      841 ccagaggagc ttgaaaatcg ctgtggactc catcaaagcc cccagcgttc caccgtgcaa
      901 cgccggaacc gaagcattgc ccacgagatg gtcggagtac tgcatgcggc ccacgacgtc
      961 gccatcgacg agctcggccg tgatccccat gaacctcgca tagggaatga cctgctgcat
     1021 ggcggtgaag tcgcctgtct cgcgaacggc agtgacggca tcgcggagcc tcattcggag
     1081 tctccgtcgt cttcgtcctg gcgccggtca tcgcgaaaca ccacgaaagt cccggaggag
     1141 gtcgcgatcg ggcttgctgc gtcgccgtgg tgcgcagccg cacgaacgaa cgcgacatgc
     1201 cgggtcactt tgtagcagtg ggcgtcgcag atcacttcct gatgaggcag cgccggacgc
     1261 acgtagtcga tgcgcaaatc gagagtcgca atacgtcgag gagctgccag cgacatgatg
     1321 accgctccac cacaggtcgc gtcgatcaga ctcgtgatgc agccgccgtg aagcaccccc
     1381 gtttcaggat ttccaacgag gtcctcgcgg taggggagcg cgagcgacaa gtgttcgggg
     1441 ctgtagtcga cgactcggag attgagagcc tgattgtgcg gaactgcgcc gacgtagcgc
     1501 ccttccaagc gccgcctgat gtcgtccttc tgagatagaa acgggtcaga cacgagccat
     1561 gctgtagcag ccgacgccta cgtggctcaa cccgccgctc gccggagcta aacgcgaggc
     1621 gggggcggag gggtcgcctg ttcagcgagc catctgtccg aggagacgaa ttcgatgccg
     1681 ttcgccctct tcaaagccag gagatcggga tggtccgtaa gcacgcgatg caggcctgca
     1741 gccttggcga gagcggtgag agcctcgtcc cagtcctcga tgaagtgact gccggtttcg
     1801 acgatctcga ccaggccccc gatggtcttc atctgcgcag cgacggcatc gtctttgaac
     1861 tccggtagcg cgtgaagcat cgattcgacc cgttccaaga cccaagagga ggatttcggt
     1921 ttgccgggac gcccgaaggc acggcgaacc gcttgctcgg gcggcgagcc gagagcgatg
     1981 gaagcgttga catagatgtc aggtgccaag acgacgtcca tgcgcgaact gtaacacgag
     2041 catcctcgat gctggccaaa atgcctagaa cgtcctagtg acgccgtacc atgtccgtac
     2101 gagtctgttt cgtttgcctc gggaacatct gtcgctcccc tactgcggaa gcggcaatgc
     2161 tccggctcct cgaaaatgaa cagctcacag caagcatcga cgtagacagc gctggcacgg
     2221 gccactggca cgtcggtgag ccgcctgacc gccgctcggc aatggcagga aagcgccggg
     2281 gtctgaacat ccaagggcgc gcacgacaag tgaccgcgga agatttccat catttcgatt
     2341 atctcgtcgc gatggatcgt tcgaacctcg aaaacctctc gcggctggct cctggcgaag
     2401 gcgcgagggc caaggtcgtt ctttttcgaa atttcgatcc gagctcaccg ccagacgccg
     2461 acgtgccgga cccttactac ggcgagggcg acggcttcga aaaggtcctc gacatctgcg
     2521 aagcagcgtg tcgcgggctc ttgcaacacg tccgcgagag ccaccgacta tgaggccgga
     2581 gctccgggct gcactcgaag aacgactcgg tgctcctatc cgcgaagcga agcgattggc
     2641 gggcggagat atcaacgacg cgtacgcgct gacgctccaa aacggcaacc gcatcttcgc
     2701 gaagacgcat gcgaatccgc ctcgaggcat gttcgacgcg gaggcgcgag gtctccaatg
     2761 gatcgaggaa acaggcgcta ttcgtgtacc gcgtgtcctg ggatactccg accaacggcc
     2821 tgcgtatcta gcgctcgagc tgctcgagcc cgcaacgcgt tgcaagggct tcgatgagat
     2881 tctgggacgc gccttggccg caatgcacag gtccggggcc tcgtcgtttg ggctcgatca
     2941 cgacaacttc atcggacggt tgccgcagtc gaacgcaccg acgggcgatt ggccgacgtt
     3001 ctactggcgc tcgaggctgg agcctcagtt acggctcgcc agcgacagtg gactcgtcga
     3061 cagtgggcta cgcgcccgct tcgacgcgct tcgccacgtc cttccggaac ggctgggccc
     3121 tgcggagccg ccatctcgtt tgcacggcga tctctggggc ggaaacttgc acgtggatga
     3181 atccggacga cctgcgctca tcgatcccgc cgcgtacgga ggccaccggg aggtcgacct
     3241 cgcgatgatg cgcttgttcg ggggatttgg ggcccgcgtg tttgcggcat acgaagacgc
     3301 gtatccgctc gcaccgggcg ccgtcgaacg cgtgcccctc tatcagcttt atcctctgct
     3361 ggtgcacctg aacttgtttg gtagctccta cttgggctcg ctgaagggcg cgctttcggc
     3421 ctgcgtctag gacgcgtcac gctgacgctg cgcttcttga caccggtaaa tcagaggccc
     3481 aagtatgcac ggtgagcaag acccgcaccg acgcacggca gtctctcgag cgccgtgctc
     3541 tgctcttgat cgaccacggc agtcgacgag cccaggccaa cgcgctcctc gacgacgtga
     3601 ctgaactcgt gaagctacgc ctcggcacgg gaagcatcgt cgagcccgct cacatggagc
     3661 tcgcgaagcc aaacatcgcg gaaggattcg cgcgctgtgt agagcaaggc gcgacccgcg
     3721 tcatcgtgca tccgttcatg ctggcgcccg gtcgccacgt gtccgaggac ctaccccggc
     3781 tgatcgccga ggccgcagaa gtgcacgaag gcgttgcatt cgtcatggcc gaaccgctcg
     3841 ggagtcaccc aggtgtcatc gacgcggtcg tggatcgatg cggcgcagtt gaggacgagc
     3901 gcgagcacgg cgagccctag gggatgagaa ccactttccc cgtggttttt cgtgaggaaa
     3961 gcgcatcgag ggccctcttc gcgtccacga gtgggtaggt ctgggagacc agcggggaga
     4021 tcttaccgtc ggcgtagagc ttcgaaatcg cgtcctgggc gtccctcagt actttcggat
     4081 ctttgtcgaa atagagtccc cagtggagtc cgatgattga gtagttcttc acgaggagat
     4141 ggttcatctt ggccgaagga atgtttccgc tcgcaaaccc tatgaccaag atccggcctt
     4201 cgaacgcaac gcacttggtc gaccggttga aggtgtctcc tccgaccggg tcatagatca
     4261 cgtcggcgcc tcgtccatcg gtgaggctct tgacctgctc gacccaatcc tcatcccggt
     4321 agttgatcac ctcgtcagcg ccgtgctgcc gcaccagctc gagcttgtcg atgctgcccg
     4381 cggtcccgat cacgcgggcg cccaaggcga cgccgatctg cacggccgca agccccacac
     4441 ctccggctgc tgcgtggacc aagagcgtct cgccctccct caagtttgct cgatggacga
     4501 ggccaacgtg ggaggtttga tagacgagac ccagtgccgc ggcttcctcg aagctcatct
     4561 gcaccggcat gtggaacacc cgctcttgcg gtgcggccac gatggaggcg aaaccaccaa
     4621 actccagcag tgcgctgacc cggtcgccaa cggaaaaatg ttccaccccc tgtccgacct
     4681 cgaggaccgt gccggccacc tccgcgccgg gcgagaacgg aagctcaggg cgaacttggt
     4741 acttaccctc ggtaatgagg atatcgaaga agttgcagcc gatcgctttc acatcgatga
     4801 ccaactgccc cggcttcgcg cgaacttcgt ctagctcgtc tcgctgcagt gagcccggac
     4861 caccgagctc gcgcacgatc attctgtaca tgagccggca gtttggctgt aaaatcgagg
     4921 tacgactagt cgatcgcccc aaaggcgccg ccgtcctggt ataactggcc gatgcctggg
     4981 caaaagcgaa agcactcgtt gccggcgggt gtccttccga caaagcggga tgaggccgcc
     5041 gaggatgcgg gctctcgaat tcgggcgagg gttcctgtcg aggcgagcga acgaccatcg
     5101 agccctgcgg agccaagcca gaccccagcg gaacgcgcgc ggaccctggt cgacgaactg
     5161 atggcgctcg cgcccaatga gatcgcgcct gtcgtacgac ggctgctccc acttggaaac
     5221 ttcgcgctcg aagagctggc aagaaggttt ccagggccgc tctggcgccc ttccctggcg
     5281 accgattcga ggctgccgcg tcccgaagag gtctccgcga tcgcggcggc catcgccaca
     5341 ttcgaaggag aggccatcga gtacctcgcg cgtctgatgc aggatccaag cgcgaaggtt
     5401 cgctactacg ctgccgtgat ctgcggtgcg tacgcgaacg cagagctcat cgatcctctg
     5461 atccaagctt cgctggacga agatcgggag tgtcggcgcg tcgcccttca tctgctgagc
     5521 gcctaccagc atgaaccgag gtacggcgtc gggcttgcaa gcctgcgtcg ctgtgcgtct
     5581 gatctggaat gtccagtccc ggtgcggcgg cgtgccatct ccgcgttgac ccaacttcgt
     5641 gacggagctt ccgcgccgct gttcgtggac ctgcttggcg attccgaccg cggtatcgcg
     5701 acggcatgcc gcgtggggtt gcgcgttctg acggcccacg atttcgggtt ctcgcgggat
     5761 ccgtggcttc ggtggctctc ggaacgaggc caagagattc ggattcagtg gctgatcgag
     5821 gggctcggag acagccgcgc gaacatccga ctcctcgcgt cgcgtgagct ctggcacctc
     5881 acgcgcttcg cacaaccctt gtctgagacg gccacgcgcg acgagttcct agcagttcaa
     5941 aggaactaca agcgctggtg gtcgagcaag cacccgacat gatcagagaa agccggcgtt
     6001 tccgagagag gtgcgcagac ggccaacccc ttcgaaaacg agcgcgtcga tcccttgctc
     6061 tcgagcgact tccacgttgc cggcgcgatc gtcgatgaac aggcattgct ccgcgggaac
     6121 agcgagctca cgcatgactc gtttgtatgc ctccgtgtcg ggcttcctca cccccgtcac
     6181 gcaggaaacg aaggtccagt cgagaaaacg ggagacgccc aggcgctcct ctatcaatcg
     6241 gtaccagatc ggatagttcg agaaggcgtg catctcgaag cctttggcct ggagctctac
     6301 caagaggtcc tcgagtcccg gcagccaacg gtaggacgag cgaaccgcgg cgacgaaacc
     6361 cgcgtggtca aagccccgac catccgcgaa gaagcgccgg aggaactcgt cttcggagag
     6421 ctcgcccagc tcgaactgca cccaggcgct cggatgcttg gcttcgagga gcgattcgaa
     6481 gctcatgcca aagaacgcgg gcatctcaga gaagaacgga tcgtggacca aagtacccat
     6541 gacgtcccag aggagaattc ggcgcatcag atcatcctat cgcggatccc ccgagatcct
     6601 cctgggggcg ttgttcgagg ccatgccgcc ggagcttgtc gagaaaagtg gttcgcttaa
     6661 gtccgagcat cctggcggct tcgctcttgt ttccacctga acgcgagagc gcatactcga
     6721 gaagccgccg ctcgacctcc aagtaggttc cgtcgaggga cccctctcgg gcggcgcccg
     6781 gccaggcaag cggcggaagg tcagtgatgc tgatcgtttt ggacgatgtt ctgccgaccg
     6841 cgagctcgat gaccaactcc agctccgcta cgtcgccagg ccattcgtgg tcgactaggg
     6901 cgaccatcgc cgcttgatcg attccaacgg gctcgcgagc aaggacccta caggctcggt
     6961 cgatcgcgag cagcgcaagg gacggcacgt cttcgcgccg atcccgaagc cgaggtatct
     7021 tcagacctgg acccgaaaaa catcgcgcca ggtccggatc gagggcgccg cgacgctgaa
     7081 gctcgatcaa ggtcgtgcgt gaagtcgcaa tgattcgagg gctgacttcg ggcgtctgct
     7141 ccccttcgtt ctcggtctgg ttatgctcga tcagggatga ggcgaggcgg gcctgcgcct
     7201 gcttgccgag agccgggatg tctcggagca gcaatgtccc cccgatcgcc gactgaaacc
     7261 atcccgcgcg atgctcgctc tcggagccaa agagaagcct catcacctgc tcagggggag
     7321 cagcggaaca atccgcaacg atgaagggcg ctctccacct cggaccccgg tcgtgtatga
     7381 atcgggagac cggcaagacc ggggagcccg catctgcgac gagcaatatc ggtttggtca
     7441 gaggcgctag ctcgattgcg cgcgtctgca cacgcctcat cgactggctg tatgcaacgt
     7501 gcagagtttg gtcttccgcg agtccacgcc cgagaacatc acactgctgt cgaagctgct
     7561 gcacctctgc ctggagcgcg gtgatcctgt cctctgcagc gcgccgctcc gcggatatct
     7621 gatgaatgcg ggtatcggcg cgccgatgcg cgagcgcggc ggccagcgaa ccgccgagtg
     7681 tctccgacaa gcgccgaagc tcctcgatct cgattcgcat ggctggctca ctgcgatcgg
     7741 atcccggaag gacgagcagt ccctcgatgt gttcgaggtg ctggcacgga accaccatac
     7801 cgatcccctg gtcttgcatc gagtcgacga gcgttcggac catgggctct cgaacgaccc
     7861 tgctgcgcag gctggggagg tcgagaaccc cttgcagctc gtcgacgaaa agcgcctttg
     7921 cgatggcagc gggggcatcc ccggacttca cgttggctcg ctcaccgacg tccatgcgca
     7981 tgcgaagcgg cggctccaag gtgaagattt cgggcaagcc atcgctggct tcgagcgcct
     8041 tgcccagcgg aaccagagct gcgctggcga tctcctcgag tgtggcagcg ccgagcagat
     8101 tgtgcctggc tacttgcgca gcatcggcga gccggccgga ggtgctccat acggcccgat
     8161 cggagagtct gcgggcgagc gagaagaccc cccaccagag gacgcccgac gccaatccaa
     8221 gagcagcgag ctctgaacgt tcgagtggag cgtcgggcac gagtgcgtac gtgagaatga
     8281 aacacgaggc ggaggcgacc acgagcggaa cgccgagccg ccgcaaggat ctccaccccg
     8341 ggtcgagcca ggcgagatgc ccgagccaaa gtatgcctgc gacggcaaac tccactccgg
     8401 tcccgagtaa cccggtgaaa gggccgaacc aggcgtacgc aagcgctaca ccgaggctca
     8461 cgagcgccac ccccgataac acaccctcga cgccgtggcc cctcccgacc agcagaactt
     8521 gatgaaaggc gccggtagcg aggattgcag aaacgaccac gacaccgaag gtcggcgcgg
     8581 gcaggaggtt gtcgcgcgct agggcacctc cgacgaccgc cgccagagca aacccggcaa
     8641 tgacggcgct ccgcacgacg gacccggtct gcctcggcaa gaccaaatcg accatgacga
     8701 gcgccgccag cagccaggcg atcgagtgcg tgtactcgag cgaagttgag ccagaacgcg
     8761 cagaagccat cgcaatggcc aagctcaccc ccatgatggc gagcctcgaa gcgcctcgtt
     8821 cgtgctgctt tgcgcgtaga actgccaacg gtgtcaacga aagcgcgagc gacagcactg
     8881 cgaccagcca gtcaggcgag gacgcgctgc ttgaagatgc ccgcgccgcc agaacataaa
     8941 gaacggacgc gggacccagt atcgtcagaa gtctcgggat tcgcacgatt cgagctcgtc
     9001 gatgcgacga ggagggagtc tatcactgcc cgtgcgcatg accagaatcg ttcggttccg
     9061 gctcgacaaa catgcgaaaa ccgactcgct gccaccagag ccggtatttc tgttggatcg
     9121 cctcgcgctg cttcttcgac aatccgggtt cgtagccaaa gtactcctgg gtgagatgct
     9181 tgagctcatc gcccgcccgc cgtcggagcc gctcgtcgga gtgcatgagc ccgtcgatca
     9241 gccattcgac gcgatggcgg tcaccgtgct tccgaagcca gcttgcccat ttctttcggt
     9301 gagtgccaaa atcctgacag ctcagacgga cgaggctcgc atgtgccgcc ttgacgatct
     9361 cgatctccgg gctctccagc tgcgagatca aaaaatcaaa cgaatcggcg tcgcgcagct
     9421 tccctagcgc ttcaatggag gtaacttgcg gtcgcagctc tcggtcatcg cgtgcagaag
     9481 cccggagccg ctcgatcaac tgtgtgaact cgagctcgca agcatagagc acgctcagtg
     9541 cgcgataggc gctgttgcga gctcccgggt ctgagtcgaa caaacggcga ccgatgggcc
     9601 gcaccaggtc agggtgcacg aactcggctg cgaggacggt cgcgtagtag cggatgtcgg
     9661 ggtcctcgtg atccagtagc gggatcaggt acggcaccga tcgtcgcccg aacgcgacga
     9721 acgttcgagc gatgggcgaa atgtcacgcc ccgcaggaat ccgagcatgg ggctcgttgc
     9781 gatcgaacca gaggttcccc gggaactccc gcgctagcgc tggaagcgcg acttcgccca
     9841 ctgccaagag cgggaggatc agcatctgat cttcaggtcc tgcgtcgcga agcgcagcaa
     9901 cgagttgttc gacgtcgtcg ccgacatcga cgatcacgct tggaagcgaa ccgttcgaac
     9961 cagagggcgg tggtgcgggc gcccatcgtc ttccggcagg ctcgctatcc gcgttcggtg
    10021 gggccgagct cggcccgtgt ggacccaagg acaccacatc gtgcgagatg gcgcttccgt
    10081 cgttgtcacg ccggggatcg ctcggggcgg aacgccgctt cggggtcgat ctcggcgcgg
    10141 ctgctggatg ccaaccgcca aacgggtcct ggtttgggct ttgggccggt ctagaagcgc
    10201 gctttcgtgc gaatggctgc ccaagtacct cgggaggacg cggcggaagg ctgtaccggg
    10261 tggcgtctat gaagcccgac ttggggtccg agcgtttacg cggaggcgca cccggatcgg
    10321 agtttctgaa ggtgccagca ggaaccgacg agcctatctg cgggagaatg tcgggcgggg
    10381 gttcggtatc ggccatcccg acgggagccg gaatcgaaga atctccccga gggtacgacc
    10441 cggagacgtc cttcttgggt ggaagcgagc ttcgcttgcc aaccccttca aagggcgcga
    10501 cgtagggaat cgaaccggca tcggccgcat cgagctgatt gcaaaggtgg cgcattcggg
    10561 ctgtcatctc gatgtcgtag tgacgggcca atccggcctc cacccgcacg gtgcacacca
    10621 cgcgctgcac ggggtcaata ccgagctcga acgatatttc gcggagggtg gcatctggca
    10681 aggggcgatc caccgccagc acgaccgcgt cctcggtcct tgcaacgggg atcaatcgat
    10741 gccggacggc gaactcccga ggcagaactc ggatggagct gatcggcgac tgcatcactt
    10801 cgttccgtgt ggccggaaga agtccgaaca ccgccgcgca gtaggcgttc atctcgttct
    10861 cgcggagcaa gccgagctcg agaagcacgg tatcgaggtt tccaccgcgc atgacctgct
    10921 cttggatcgc agcttgaacg cgatcgacgg cgatcagctg gtcacggacc agaagcgagg
    10981 cgagaaccga catacctttt gcaatggtgg ggccgcttgc gccgctgtgt caacgagctt
    11041 gtcagcacgc cgcgagcctg tcttggacga tggcgagagc tgccggaacc gcggtttcaa
    11101 cccgcaagac cctggccccg aggctcgcca cgctgaagcc cgctctttcg aaacggttga
    11161 tctcgccatc ggtgaaaccg ccctcgggtc cgagagcaca ccacaattgc tgggggatga
    11221 actcgagttt cagggcgcct tttgccctcg caccgaagag aactccgcaa gcacccgtgg
    11281 gcatctcgtc gagccagtcg gtgtagggtc ggggagcgcg aacgatcggg acgtcgttgc
    11341 gctcgcactg ggccgcagct tcgcttgcga tgcgagtaag acgatcgatc cgggagcgct
    11401 cgcgttcggg gtcccagcgg gggacggtcc gttcggtgag catcagggcc acctcgtcga
    11461 ctccgagctc ggtcgccatg cgtacgcaat cgtcgagctt cgatcctttt ggcaccgcga
    11521 gcatcaagac cagtcgcacg cgtcgctcct cgctcgcgga cggcggctct gctttgcaga
    11581 cgatctgctc accgacgctt tcgaggcgag ccggcgcagc acggcctctg ccatcgaaaa
    11641 gcaagagctc ttcgccgaca cgaagacgaa gcacgcgaac atgacggctg gtcgattcgt
    11701 cgagcgtcac ctgtccgccc cgctcgggga gatcgggcac gtaaagcctc cggacgctca
    11761 tcgcagcccc gcgctgagca cgcaggcgca ccattcgcct tcctcgatga cggactcgag
    11821 gtgcatcgat gcataggcgg caagcagctc gtcgcgctcg acgcgaagaa tgccactcag
    11881 gaccagaatg cctccgggcg cgagacgcgc ctccagctgc tctggcatgt ggaccaagat
    11941 cctggtttcg atgttcgcga gtacgatctc gtagagccct gtgatttcgg ccaaaggacg
    12001 cgttgaaacc tcgattcgtg gggcaaccgc gttgagcgca gcgttgcgaa gggcaacgac
    12061 caccgcgtcc tcttcgatgt cgatcgcgac cgcggacgcc gcgccgagac gaagagccgc
    12121 gatcgaaagc acaccgctac cgcatcccac gtcgagcacc cgttcgcctc cggcgacgcg
    12181 ctcgtcgagg atctgcagaa cgagccgggt ggtttcgtgg tcaccgcttc cgaaagcgtt
    12241 ctcgggatcg atgctgagga ccacgtcacc aggtttgctc tctactttct cccaagacgg
    12301 atgcagcaag agtcgtttcc cgatccgctg tgcgctaaag ccacggcgcc actcgatggc
    12361 ccagtcctgc tgggcttcgt aagtgatctt ggcatcgtac tcgtcgcgaa gttcctgcag
    12421 ggcgtatcgt gccgacgctt catccggaaa cgacgcgatg atcatgggca gcgcaggggc
    12481 cggctcgcgc accagagtcg tctcgtctcg ctcttcgaga ccggtggcgc cgagctccca
    12541 taagcgtagc tcggccagct cgattcgctc cggctctacc cggacggtga cgaatggatg
    12601 gtgcgaagaa tcagacatca gatctcgatc atgcgcgcgg acacatagat gactaggaac
    12661 agaacccagg tgatcgggcc gagtcggttt ccgccgcgcc aggctacgat gggagtgaac
    12721 accggtacga ggaacccgag gtaccgccac tgcgctgcca acggcccggc gagcacgcgc
    12781 cacgccatca gtagatgcgt gaagaggagc gcagcgccag aaagagccca aagcgcgagc
    12841 aaccagagtt ccacaagccg ggttctagcc cattgtgggg agtctcccaa tcgaagctcg
    12901 cgggtgaatc ggtcgccgag tctgctacct tggagtttcg gaggtagttc gcttgtcttc
    12961 tagaaccatt cgcatcggtc tggtgggttg cggggtcgtc ggaaccggcg tcttgaacct
    13021 cctgcgcacg aacgcggcaa ccatacagaa gcgcctcggc gcggccctcg ccgtcgagca
    13081 gatcgcggtc aagaacctga agcgcaagcg gtctgacctc gtcccccggg agatcctgac
    13141 cgacgacccg ctggccgtcg cccggaatcc gaaggtcgac atcgtcgtgg aggtgatggg
    13201 ggggatcgaa cccgccgagc aggtcgtgcg ggaggccctc gagcaaggca agcaagtcgt
    13261 cacggccaac aaggctctgc tcgcggagaa aggcgatgcc atcttcgatc tggccaagca
    13321 gaagggtctc gacgtgagct tcgaggccgc agtggcgggt ggcattccga tcatcaggac
    13381 gttggaggag tctctggcgt cggacgagat cgtctcggtc caagggatca tcaatggaac
    13441 gagcaactac atcctcactg agatggcaga gcgaggactc gatttcgacg tggcattgaa
    13501 gtcggcgcaa gcagcaggct acgccgaagc tgaccccacg ctcgacgtca acggaggaga
    13561 cgccgcgcac aagcttgcca ttttggccac gctcgcgttc ggagtgcgcg ttcatcctgc
    13621 gcacatcgca accgaaggca tcgatggagt agatgcgtct gacatcgaat atgcagacgg
    13681 cttcgactac gcgattcgac cggtcgctgt cgcgtcgcgc agtgcgggca acgttctcga
    13741 cctccgagtg catcccgctc tggtgcccta cgacaacccc ttggcgaata tttccggcgc
    13801 gctcaatgcg gtctcggttc gcggcgaaca ggtcgggaca tgtctcctat ccggaccggg
    13861 cgcgggggcc ggacccactg caatgagcgt cgtaagtgac gtcatcgacg ttgcgcgcaa
    13921 cattctgaag aatgcgtcgg gacgtgtgcc gagcttcatc cacgacgggg atgcggaacc
    13981 gctcgtgcag gacccaaacg acctccgggc gtcgtattac ctgcgcttca cggtgcgcga
    14041 tgaacctggc gtgctctcca agatcacggg gaaattgagt gattttggga tttcgatcga
    14101 gaagatgttc cagaacgtcc aagcgggcgc cgagggcgcg gccacggtgg tcatcgtcag
    14161 ccacaaggcc cgagaaggcg acgtccacaa ggcgctcagc gagctcgacc cactgccgca
    14221 cactttggcc ccggcgagac tcctacgcat cctgggctga cgcagtggag actgcagcat
    14281 ctgatataag ccttacgggt gcattttgat gtaacgaact gtagagatcc ctggctaact
    14341 acacccttga ggcgtaagtg aagccctcaa gggggtatct cagtgttcaa acgttattca
    14401 tttgccatgt tgggcttgct cgccgtcgca tcgaccaccc cggccgactg tgaggtcgac
    14461 caagccgctg tgagtgcctg tatgtcaccg gttccggacg tcagcgcgag tcgcggctgg
    14521 cagagccgga cggccccgca agctgcctct tcggagctct ttttcgaggt catggcgcgc
    14581 cccacggctg ccaatctgaa tggtttggtc accattggct cgggagacat caacgacttc
    14641 tcggatgcgg cgatcctggt tcgctttgcg gacaatgggg agatcgatgt gcgcaatggc
    14701 tcgcaatacg acagcgatcg atcgatcgcc tacgtcccgg gtgtctggta cacgatctac
    14761 attacggccg acgtcgtcgc cgagacctac aaggtggagg tgggcacgtg cggtgggtct
    14821 cgtgagaccc tcgtcagccg cgcctccttt cgaccggatg cgccctctgg tgaaccgctg
    14881 agcaactggg ccgcatggtc ctctcaagac gccagcctcg aggtttcaac ccctgcgtgg
    14941 tctgcgtccg gcagctgtgc tcctgccacc tgtgagtctc tcgctcacga gtgcggtcag
    15001 ccgagcgatg gttgcggcgg aagcctcgat tgtggcgctt gcgcaagcgg caatgtgtgc
    15061 agaagcggcg tatgcgtcga cgagtcggtc ccggcgccga ctccgactcc gactcccacg
    15121 cccactccga ctccgacgcc tagcactggg ttcccgacct atgccacgac cggcattacc
    15181 gaggaatggt gcccccgctg ggactcgctc aaacgggtca cgagcggcag gacgatcacg
    15241 gcggcgaaca ccgtcatcga atacgagcga atcaacggaa agctgaacat cgacgcaaac
    15301 aatgtgaccg tccgctgcgt cgagatcgtc acgggcagcg acgtcaacac cttgggctgc
    15361 agcaacaacg gcaaccccac atacggatgc gacgaagggt tcttgatcga agacgccaag
    15421 gtctacacca cggggggcaa caacaacgct cccttcatgt tcgtcaccga cgaagcgtcc
    15481 ggacggcggg tccaggtgta cggcggatgc gatgaaatga agatggacgg cggctacctt
    15541 cgtgactcct tgatcgatgg caacacgaac tatagttgca acctctcagg cccacacacc
    15601 gatgtctggc aatcgagcgg ctcaaacggc ccgatttgga tggtgaacaa caccatgatc
    15661 ggaccctata agtcgcaaaa cggcacggtg ttcatcaaga ccgactcggg ccagaaccac
    15721 gactacacca tcgacggatg ccagctgtcc gggggcagct tcaccgtctg ggcccgcaac
    15781 ggcggcaacg ggatcccctt cgactggcga ttcacgaaca acacgatcat cgagaactcg
    15841 tacacgtacg gggcgttcga catcgagggc aacgggcctt cgcagtgcat cgagtggtcg
    15901 ggcaacgtat gggacgacaa gacgcccgca acgggcggac caagcaacgt gaagcccatc
    15961 ggtggctgtt cgggcgtcac gccgatcccg acttggtcgg tcggtggcct cgccttgacc
    16021 ggcacgtctt gtacacgggg cggagcagcc tgcgacaacg tcgatctccg ggcttcggcg
    16081 tcgggcagcc aaagcggagg ctcccctcgc tggcgcttca actgtggcgg ctcggccggc
    16141 aacacgggcg gcaaccatcc cgagggcgca gatctttggt acgactacac gccgtgcaac
    16201 ggtcagagaa gctgcacgat gactgacgtc tgcgactaca gcggggaagc ctccggcacg
    16261 taccgagtgc gcatctactc ggagagcggc ccaggctcgc ggactcgcat cagcgacacc
    16321 tccgaggcca gcttcgtggt caacaactag gcccgaggag cgtaggaaca acaagcgccc
    16381 tgtccgcgcg gctattgacg ccgcatgaca tagcgatttc gcgacgccac tgacgtcgca
    16441 gcgctacaac tcgacgaaac gggttaccgg ctcgggctcc ccgccgcagc ttgcgggacg
    16501 gtcgatgccg gtggactcga gcctcactcg aacgctcgtc tcgccggatg tggttcgaaa
    16561 tcgcgcgacc caggtcgact cgtcttctga atcgctcccg atgagctcga gcgcgcttgc
    16621 gtcctgcgcg ctgagcttgc gtcgcaagaa gatttcagct gcctgcgccg gccggtcata
    16681 tgcacagcgc ccgcgaaaga gatcgatgtt gcccagctcg ccggcttggc gcgctgcaac
    16741 gagtgccggt gcgtgctcgg cgcgtagcct cccatacatg agtccgtcgg gaagccaaac
    16801 cgcacaggcc gcaaaacgat gtccaccaag gtgactcgtc tgccaggtgt caactccctg
    16861 cccttgaatc gcgcggaaga cggaacttcc atgcagcgcg caacagcggt cgcgccgccc
    16921 gtggacacac accaagcaga tcggcgcaag ggcttcggca gggagtgcat cgagatcgac
    16981 gtctaggagg tcttcgtact ggtcgagctg gagcttcgac gattcctgcc gatccgcccc
    17041 cgaacgcacc tgcatgatca acggacgctt gccggagcgc ccggggcgcc gaatgagctg
    17101 aagacgcgag cgtggcgttc ccagccagtc gccgagcttt gcgcgcaccg tcgctggaag
    17161 ggcttcggtc tgaagcgcct ttggagccca gggctcggcc acttcgagca agagccatcg
    17221 gtccaccgac tcgaccgcgg tcgcagcgag agtctcaccc gcttcgagcg atgcaaccga
    17281 gcaggcgtta agattcacgg ctagtccact tgcacgggaa ggggccgtgc catctcttcc
    17341 tctgcttgat cgatcgtcgt ctggcggcgg gccgcgatct cggacctcca agccaggtac
    17401 ttcgattcga ggctcgcgag ctgctcatca gcgcgcgcca attcgctttc gagccgttct
    17461 cgtttccgcg agtcggtcgc ctcggcaagt gctttgccaa ccgcatcccg aactagcctc
    17521 atccttgcga ctgcgtcggt gaagttctca cgctcggcga atcgccgcgc ttgcaccatc
    17581 caaacggccc aacgttcttc gtcactgctg agtttcatga gatctccaat ttacggcgtt
    17641 tcctcgggcg gtccagtcga gggcctaggt cgatggtaga gccaccagtt ctcgaagtgc
    17701 gcgacacgct gaatgctcgg atccgagacg aacaagaagt ccggggagct cacccgacgg
    17761 accaggaacc agtcgaagaa gtcgccgtgc tcctcgagct ggaactgctc tggggtccat
    17821 tcccagcgca cgggggtctt cggtggcacg accgcattgg gatctgaagg gtcgcggtaa
    17881 cgaaacggga acgtcccgag ttgggcgaaa tgaaagctca gccagccgcc gcgctcggcc
    17941 tgcacatagg cgggcaggtg aatgtagggg ctgtttcgcg cgcgtgagcc ctggtggtcg
    18001 tagacgaggt agaggagctt tggggccttt ggaagctcgc gaacgatttc acggaagtga
    18061 acggtgctgg tcgagaactc gcgatgggct tcggcgacga actcgccaag cggcgcgatt
    18121 ccaacggccg tccagatgac gaagccgaga tgcgcccagg tcttccgcgg caagttgggc
    18181 aggagcgcgg gcagcaggaa cagcgcggcc gtgatctcgc ggggaaaaac ccaccaccag
    18241 attccgagct ccatcgggag cgtcaggtag aggcctagga gaaccagaat cgcgactccg
    18301 acgacggcgt gggcaggaac gacccagcca ccccgcggcc aggagcgaag tcggagcaag
    18361 gcgatcgcca agagcccgac ggccgtcacc atgaagagca agccggtgcg tcggaacatc
    18421 gccgtatcct cgtcgccggt gaaaatgtcg gtcgtatagg agctgatttc cgacatgcga
    18481 ttccacgccg gcggccaggt ccagctcatc gcgggcatga gaaggtcgac ccgggtgagc
    18541 caccatgtag tgaagagcgt ccccgccacg gcgacgggga ttacgagtcc gcgaatgctt
    18601 tcggcgcgcc cctgcatgac cgcgccgacg acgacgagca tcagaagagc aaacgggaag
    18661 cggtagacat gagtgaagaa gagcaaaatc aggaccgcga tgagcgcgcc ctgcaccttg
    18721 atgctcggtc ggtccacgac ccggagagcc aggccaatcg ccatggcaaa aagtccgagt
    18781 gctccaagaa agcccacgaa accccaatgc gaggggaccg tccaaaccgc gaccagaccc
    18841 cagagcccga gcagcgggga cttccgtagt ccccagcaga gcaccatcag acccaaggga
    18901 agaagagcga gaagcaacgc ggctgcggct ttggtggcga cgacgggcgg gagcagcagc
    18961 atgaagatcg ccgagaccgc gtagagcgtg acgtacggga cggcaaacat ttgaaacacg
    19021 aactgctcgt ggaaatgcca acccgggtcc gcgtagtgac ggaacaccga ggacgacgcc
    19081 gtgtgaaacg ggaagtccgt catcatcgga tagtcggcta cccaaaatgg cgcgccgacc
    19141 acgtagacga tggtggctcc ggcacagagc catgcgagcc acagcagtgg cgcgggcccc
    19201 gtcatccgtc cagacggatc tgtacgcccc cctggcatcg gcgccaccgt atcacgaaca
    19261 ggccgtcagc ccttgagagc ttccgcgccg ccgatgatct ccatcaattc cttggtgatc
    19321 gcggcctggc gggctcggtt gtactgcagg gtcagacgat cgatcatgtc ggcggcgttg
    19381 ttcgtcgcgg agtccatggc ggtcattcga gcgccgtgct ccgaggcgac cgactcgagc
    19441 aaggctcggt agatctgaac ctcgacgtgc atcggaagga gacgatccag aagcgcagtc
    19501 tgattaggct cgtagatgaa atcgcccatc cgatcgtcgg caagctcgag cggctcgatc
    19561 ggcaggaagg gctcgatcac gacctgctgc gtgatggcgc tcttgaactc gttgtaaacg
    19621 agatagcatc cgtccagatt gagcgaggtg tagtcctcga cgatgtatcg tccgatttcg
    19681 cccgccttgt cggagctcag gtcttcgaag accccgagga agttgtggcg aatgtcggcg
    19741 ttccggcgag tgaagtagtc ccgtcccttt cgaccgatga tgccaaaaca gacctcgctg
    19801 ccctgcgctt cgagctgctt ccagcggccg tacgcagcct tgcaaatgct cgagttgaac
    19861 gcgccggcca aaccacggtc gctcgtgagc accacgagca tgacgttctt cggttcgcgt
    19921 cgcgcgagca gcggatgaac atcctcgtcg gcgcctaccc gggccgagac tccgctcagg
    19981 acttcatggg tcttgacggc gtaagggcgg agacgctcga tgttctcctg cgccttccga
    20041 agccgagcac cggcaaccag cttcatcgca cgagtgatct tctgcgtgct cttgacgctt
    20101 ccgatgcgct tccgaatgtc cttgagattt gccatcgtcg atcagttgtc ttgaacgggc
    20161 tcgaagatct cggcgaatgc cttgagggcg tcctgaagct ggctttcgag ggtgtcgtcg
    20221 atcttgccct tcgaaacgat gtcggaaagg atcgcggcgc gctcggcctt gaggtacgag
    20281 atcagctctt gctggtagcg gccgatcgat tgcgggggaa gcacgtcgag gaagccatgc
    20341 gtaccggcat agatgaccac gacttgctct tcgaccgacc agggcacgaa ctgaccctgc
    20401 ttgagcacct cgacgaggcg ctcgccgcgc gagagctgct gccgggtcgc ggcgtcgaga
    20461 tccgatgcaa actgcgcgaa tgccgccatc tcgcggaact gcgcgagctc cagacggagt
    20521 gtgccggcca cctgcttcat ggccttgatc tgtgcgttgc cgccgacgcg cgaaaccgag
    20581 atgccgacgt tgatggcggg tcgaacaccg gagttgaaca agtccgactc gaggaagatc
    20641 tgcccgtccg tgatggaaat cacgttggtc ggaatgtacg cagagacgtc gccggcctgg
    20701 gtctcgatga tcggcaaggc ggtgagtgag ccgccggaat ccgggagctt ctttgcaacg
    20761 aagccggcgc ccttctcctt tgcagcggcc tgagcctcgt gcttcgactt cggtccggtg
    20821 tagaccttgt tgccgagcct gctgggctcc tggtcttctt tcacgacgac ccagtcctcg
    20881 gccattttgg cggcgcgctc gagaagacgg ctgtgaacgt agaacacatc gcccggatac
    20941 gcttcgcggc ccggcgggcg gcgaagaagg agcgaaagct gacgataggc gacggcctgc
    21001 ttcgaaaggt cgtcgtagat gcagagcgca tgctgaccgc tgtcgcggaa atactcaccc
    21061 atcgtgacgc ctgtgtacgg cgcgatgaac tggagcggcg ccgactcgga cgcgccagcg
    21121 agaacgacag tggtgtactc catggcgcca aactgcgtga gcttgtcgac gacctgggcg
    21181 accgtcgact gcttctggcc gatcgcgacg tagacgcaga atacgccctt gcccttctga
    21241 ttgatgatgg tgtcgagtgc gacggcggtc ttgccggtct gccggtcgcc gatgatgagc
    21301 tcgcgctggc cgcggccgat cggaatcatc gagtcgatgg ctttgaggcc ggtctgaagc
    21361 ggctcgctca cgggctggcg agccacgatg cctggagcct tgatctcgac gcggcggttg
    21421 agcttgcttt cgatcggccc cttgccgtcg atgggctggc ccagagcatt gacgacgcga
    21481 ccgacgagcg ccgggcccac cggtacctcg acgatgcggc cggttcgctt gaccgtatcg
    21541 ccctcgttga tgccaacgtc ggagccgaaa atgacgcagc cgacgttgtc ctcttcgagg
    21601 ttgagaacca tcccgtacac gccaccgggg aactcgagga gctcgcccgc catggcgctt
    21661 tcgagaccgt acaggcgggc gataccgtcg cccaccgaca gaacggtgcc cgtctcgacc
    21721 acatcgaccg acttctcgta agaagcgatc tgctgcttga tgatgttgga aatctcttca
    21781 gcgcgaagtt gcattttgca aggctcctga ttcgatgcgg ttacggcgtt tcgccattcg
    21841 caagcaacgt ttccctaagc tcgctcagcc ggttgctgag gcttccgtcg aagacctggt
    21901 ccccgacgcg cgtgaccacg ccaccgatga gcgaaggatc ttctctcttg acgagcacga
    21961 ccttacggtc gctgactcgc tgtagtttct cgacgaggcg cgcgtagtaa gcgtcgctca
    22021 gaggcttggc gctgacgacc tcgacgcgta cgcggccggt ctctgcttcg gcaagctgct
    22081 cgagtgcttg aagcacgtcg tcgaggtgac cgaggcgtcc cttgtcggcg aggaggttca
    22141 ccgtgttgcg caccaacttc gagcaaccga gcttctgcat gaccgcgtcg acagccgcct
    22201 ttagtgcggg cctggggatg acggggtttg cgacgatctc gcggagctct ttgctagcct
    22261 tccaggcgtc ggagagcgcg cccacgtctt tgagcgtggt atcgagctca ccctgctcac
    22321 gggcaagatc gagcaaggcc ttcgcgtagc gtcgtccgag ggcgctcatg atttccgctc
    22381 ctcgagaacc tcgccgaggc gagtgaggta ggcttccgcg agctgatctt ggtcagatgc
    22441 gctggtcttc tcttgaagga gctcctgggc ggcgaagacc gccgcgttgg cggcctgatg
    22501 cgtgagctct ttgcggagct gcttgatctg ttgctcgatg aggaagctcg tgtccttgcg
    22561 cattcgggcg gccttctctt cggcctgctc gacgatgcgg tcgcgctcgg cctcgccggc
    22621 cttcaccatg tcgtggcgaa tttgagccat ctcttggtcg agcttctcga gacgcgttgc
    22681 ggtttcccgg tgccgcttct cagcttcggc gcgaagcctc tgcgcctcgg cgagctcgtt
    22741 ttccacctcg gcgcgacgcg cggccagggc cgggtttccc ttctttcgga tcacccaggt
    22801 aagaagacca accagaacca ggaagttgat gaccgtgcct tggaacaccc ggtccttgag
    22861 caggccagtc acggtgagct cgccatggtc tccatgggcg tcgtggcccg actgctcttc
    22921 ggcggcctgg tgggcctcag actcgtcgta tgccgcctcc gctgagacga ggctcggcga
    22981 ggtcagggcg agcatgagaa acatggcgcc gagcaagcgc atcactggac ctcccggttc
    23041 agcattttcg atgcgatctg ccgtgcaagc accgggacgg actggtcgat ctcggcgcga
    23101 aggcgagatg cctccttggc cagcttggca tccgcttcgg cgagctgctt gcccgtctct
    23161 tcgcgaaccc gatcgaggac ggtgcgctcg agacgcttgc cctcgttgcg aagtcggtcg
    23221 cgctcctccc cggcttgaag gcggatacgt cgcatctcga cctcgaactc ctcgctctcg
    23281 gcggatgcct cgtcctgcag acggagtgct tcgagctttg atccctcgat ggcgttttca
    23341 cgcgcttcga agagcgcgac catcgggcgg aagatcaagg gtcggaacac gaagaaggca
    23401 accgcgaaga gcgcgagctg aatccagatc gtgccgtcga ggtcaatgat ggaaccctca
    23461 gacaaaaggg ctgtgaagag cacgttcatc gcagcggtat tcccggtcgg ggctagagtc
    23521 cacgcaggag agactccaag aggagaaaaa ctccaaacgg cgcttggggc cgaatcgcag
    23581 ctccggcaag gacaattccc tagcggcgcg cgttcgtagc atgggcccga acccagtcaa
    23641 ggacggtcga gctgcgtacg agctctgcgc tcaggtcgat ccgcggggac cttcgaacac
    23701 acgggttcga agcgctcgca gtgtctttcc tgcggcgcgt tcgttctccc ggctgaacac
    23761 cgaggacgga agcgggatat cgggatcgac gtagatcttg aagtcgaccc gcgtgcgcgt
    23821 acccgcgtcc accggtgtga gctcgaagcg agcgctgaac gcatcgacgt tgccttccat
    23881 cagatacgcc tcgacgacgc gtgaatcgcc ttcttgaggc cgctcggcga ggttcagctg
    23941 gccccagagc gtgaacgcac ccttggcgac gctgacctcc atgtagacca tggcgcggct
    24001 gccgcgctgc gagagaaccc tggatttcgt aaaattaggc atgaaatccg cgtagttggc
    24061 gtagtcctga acgatgggca ggacctcatc gatcggcctg tccaccacca gaacagcctg
    24121 accccatgcg atgttggagc cctcttcgag gatctgcgcg gtgagaatgc ccgcgttttc
    24181 gttgggctct tcggctgcga gcgccgggct cgcagcaaca gcgaaaatcc aggtggcagc
    24241 ggcgatccca atcaagctct tcttgttcat ggcttcatct ctcttctcgg agctgtctac
    24301 gccgagcaac ctggtgacat tacgtcacct tgtctagtcc tggagccgtg gatcgccatt
    24361 ccgccaggcc cgggctacaa gctatggcga gaaaatcgag cggttcgcgg acggtttgct
    24421 agtttgatcg atgaatgcgc ccgacactcg gagtcagcgt ttttgctgcg cttttcctgg
    24481 tcatcgggat gcatggccgg gctagagccg ggcctgagcc ccggtcggag gggtccgcac
    24541 gtggtggtac tcctgcagac atgcagcctg aaacgtacga acggccggtt ctgaccgtct
    24601 tgggcggggg tggcccgcgg tttcagcgga tcaagctcga agtgggcgac ggcgcgggcg
    24661 gcatcgagcc tcgcgacttt cagacgggcg cctacttcga cttcggctgg tttgtcctgg
    24721 cgcgacccgc ggctcgcaag ctgtcccgtc cgagcgtgca ggcgatcgtc cttcaactcg
    24781 agggtggcgc gggcgtgggt ctgacggtgg agccttcggg aagtgggatt tccctacaga
    24841 cccgggcttg gcggctgctc ggacaattcg gctacctcta tccgatgggc cgcgtgcaag
    24901 tcggtgggct gctcggggtc ggagccgatc tgctgaagat cgatctgaac tcggtgcttc
    24961 cctcttcgag gatcatctat ttcaggctgg gaccggcggt gacctatcag ctcctgaagc
    25021 agctcttgga cctccgcgtg gacctcggcc tgcgcgctcc attcgtcctc gacggtctcg
    25081 cggatgcttt cgggaacgac gcccgcggca ttggaatgga cgccgcgctg atgttcaacg
    25141 gacgcctcca gaacgggctc agctacgcag tccgattgat ctgggagcac tactggctcc
    25201 gtttcaccgg cacgagcgcg ggcgtcccgg cgtcgggcga gggcgccgag ggcggcgaca
    25261 acgcgatcac gcttcatttc ttggtaggct ggtccttgta gccgtctggc gaagtatcgt
    25321 cggcggcgcg cggcgtgctc ggccgctcgc tgagctcgat gacttcgccc atgacgcagg
    25381 agccgtcgaa caccacgcct ttttccagga atagcacctg ggtccgaatg tcgcccttga
    25441 tgcgggacgg agcgtgaagc tcgacgagcg cggtcgcgtg gatatcgcct tcgatctgac
    25501 cgccactgac gatcagactg tgcacgcgga tggacccttt cacgcgggct tgctgtccga
    25561 cgacgagcag cccttcgctg aatacctcgc cctcgaactc gccgtcgatg cgcgcccgcc
    25621 cttcgaaaaa gagctttccc tcatagcggg ttcccgcacc gagaagcgct tgaatgacgg
    25681 tgtcctggtc ctgcatcgca gggtgcagcg tagaaagccg cacccggggg gtcaattgag
    25741 catgcatgag ggcttgccac gagtcaccgt gaccctagtc cgggagagct ggcgtcgttg
    25801 accgccccga ggggccgtgc taggtcgcgc tcgctttaca ggcgggatgg accgccggcc
    25861 gacacacttc catacaaccg gaaagaacat ggcagagctt cagaacggtc aggttactca
    25921 agtcattggt cccgtcgtgg acgtccgctt cccgcaaggg agcctcccgg agatcctcac
    25981 ggcgatccag gtcaccaacc ctgcgatcaa cgacaaggat tggaacctca cgttggaggt
    26041 tgcgcagcac ctcggcgaga acaccgtccg tgccgttgcg atggactcca cggacggtct
    26101 ggtccgaggg atggccgtca agaacacggc ggctccgatt tcgatgcccg tcggcaagga
    26161 gtgcctcggc cgcatcttga acgtcgtcgg cgcgccggtg gatgagctcg gcccggtcaa
    26221 cgcgaccaag ttctctccga ttcacaaggc tccccccagc ttcgtcgacc agagcaccga
    26281 gatcgagatc tttgagacgg gcatcaaggt catcgacctt ctcgctccct accgaaaggg
    26341 cggcaagatc ggcctcttcg gcggcgcggg cgttggcaag acggttctca tcatggagct
    26401 gatcaacaac gtggcgaagg cgcacggcgg tgtgtcctgc ttcgcgggcg tcggggagcg
    26461 aacgcgtgaa ggtaacgacc ttctcctcga gatgagcgag tccaagctcg aaacgggcga
    26521 gaccgttctc tcgaagacgg cgctgatcta cggccagatg aacgaaccgc ctggcgcgcg
    26581 tcaacgagtc gcgctgagcg cgctcacggt tgccgagcac ttccgtgatg aagaaggcca
    26641 ggacgttctt ctgttcgtgg acaacatctt ccgcttcacg caggcgggtt cggaagtgtc
    26701 cgcgcttctc ggccgtattc cttccgccgt cggttaccag ccgacgctgg ctaccgaaat
    26761 gggccagctc caggagcgca tcacctcgac caacaagggc tcgatcacct cggtgcaggc
    26821 gatttacgtt ccagccgacg acttgaccga cccggcgccc gccacgacct tcgctcactt
    26881 ggatgccacc accgttttgt cgcgttcgat cgcggagctc ggtatctacc cggcggtcga
    26941 tccgctcgat tcgagctcga ccatgctcga ccctgcggtc attggtgacc ggcactacaa
    27001 cgtcgcccga ggcgtgcagg agaccttgca gaagtacaag gacctccagg acatcattgc
    27061 gattctcggt atggacgagc tcagcgaaga cgatcgcatg gtcgtcgatc gcgcgcgtaa
    27121 gattcagcga ttcctgtcgc agccattctt cgtggccgag cagttcacgg gcctcaaagg
    27181 cgcctacgtg ccgctcgagg agagcatcgc cggcttcgag gaaatcctcg acggcaagct
    27241 cgaccacatt cccgagcagg acttctacct caagggcaac atcgaccagg tcaaggaagc
    27301 cttcagcaag cgcgcgaagg actgacgggt ccgaacgatg gcgaactcag aataccttca
    27361 actcgacgtc gtcactccaa gtggttcggt gctctcggcg cgggtggact cggtccaagc
    27421 gccgagcgtc caaggtgagt ttggcgtgct gccgaaccac cttccgctct tggccgcgct
    27481 gaagtgtggg ctgctcgttt gggaggtcgg cgggaagcga aagatcgcgg cgattggccc
    27541 cggtttcgtc gaaggcggcc ccgacaaggt gctcttgctg accgatctct tcgcctcgcc
    27601 cgagcagatc aagccggacg ccgtgcgcga agagctcgcg aaagccgaag aggcgctcaa
    27661 ggcattcgac gagctgtacg agggcccgga gtacaacgag ctccagcgcg acgtcgattg
    27721 ggcgcacgcg cgactcgaag cctacgccgc cgcccgcgcg gtctagtccg ctgcggtttc
    27781 tgcgagcgcg gcaagctcga cccagatggc tagcgcggcg ctggctgcat cctgctccgc
    27841 ttcgatcttc gagcggccgg cacccgtggc gacttcttcg tcgtggagct cgatcgccac
    27901 ggtgaactca cgctcatggt ccggcccgtc tgtccccagc aatcgataga tcggcgtccc
    27961 tcctagattg gcctgcgccc attcttgcgc acgcgacttg gcgtcacggt gtccgggagc
    28021 gcgggtgtgc aagcgaggct cgaagatccg acgcgccacc gagaacgcag cgtcggctcc
    28081 gccgtcggag tagaccgcac cgaccagggc ctcgagagca ctggccagga gccttggctt
    28141 ggagcggccg cctgacttct gttcgccctt gccgagccgc attgcgtcgc cgaggtcgat
    28201 cgcttcggcc gcctccgaga ggcccttttc gctcacgaga tcggcgcgac gcctggtgag
    28261 ctcaccctcg tctgcatctg gaaactgcag gtagagcagc gaggcgacga gcaaaccgat
    28321 cacggcatcc cccaggaact ctagccgctc gttgtcggtg gcagcgaggt cgggccgctc
    28381 gttcttgaac gacctgtggg tgagcgcgtc gagcaagagg cccgtgttgg caaaggagta
    28441 gccgagctcc tggcacaatc tcgagcttgc atcatcgagg ggctcgccgc ggttggacat
    28501 cgacccaaac ctatcagctg cgtgcgagtg aatccatcgt gaagctgcgt catgctatac
    28561 gcgctgggtg aacttccctc ccgaggagtt ggaaacgagg cggctcttgc tgcggcgccc
    28621 cacactcgaa gatgcggagg ccgcgtttgc cagctacgcg agcgatgcga agatcacgcg
    28681 ttacctcact tggagcgctc acacgagtcc tgcgcaaaca cgcgaattct tccagcaagt
    28741 gatcgatgcc tgggaccttc gaatggggca ccgcatgtgg ctcatcgagc gcaaggagga
    28801 cgctgagctg atggggacca tcggatgcac ggttcacttc catcgcgcag agatcgggtt
    28861 cgccctcgga agccgcttct gggggaacgg gtacatgccc gaagcgctcg cgagggtctg
    28921 cgaggcggcg tttgccgatc caaggatcga gcgggtccaa gccctctgcg acgaggagaa
    28981 tacgcaatcg tctcgcgtct tggaaaaggt tgggatgcaa tacgaaggtc ggctgagacg
    29041 gtacggtcat caccccaatc gctcggacgt tccccgggac tgcctgatgt acgcattgat
    29101 tcgcggcgat tgaatcgccg atcgaagccg ctacactcgg gcctccgatg cgtcttctca
    29161 ccaccgtttc cgaccagaac caggcgaaag ttctaggcga tgcgctctat gcggacggcg
    29221 tcgtgacgac tctgaaagag acgcgcgacg gttcttttgc ggtttgggtg cacgacgaag
    29281 agaagatgga acaggcgcgg gctttcttga acggctttga tcctggcgcc agccgatttg
    29341 ccgaaatggc gaagaaggcg cgcgcgaagc gaaagcaaga agccaggtcc gacgagcagc
    29401 tgcgggtgcg cacggagaag gtccgtcggc agatcgaagc gaagcagaac atgcgaatcg
    29461 gaccggtgac gactggcctg atcgcgatct gcgtcctggt cttcctcttg acgaaccagg
    29521 gtaatgacct cggcatgctc agctacttca ccttcgttcc cctggttccg gccggaaaca
    29581 gattcgtgtt cggggacgtg agcgcgatat ggcagggaca accttggcgc ttgttcacgc
    29641 cgatgttcat tcacttcggc ttcatgcaca tcctcttcaa catgtggtgg ctcaaggacc
    29701 tcggcacggc gattgaacgt gtcttctcct cgcggtactt gctgacattc gtcctgctga
    29761 cagcggcgtt ctctcacgtg ctcgagtatg cggtttcagg acccacgagg ttcggtggta
    29821 tgtccggcgt ggtctacgcc ttgtttgcct tcatgtggat tcgaggtcgc ttcgacccga
    29881 gcttccccta ccggatgccg cagcagctcg tgacgttcat gctgatctgg ctcggcctcg
    29941 gcttcaccgg ctgggtcggt ccgatcgcca actgggttca cacgggcggt ctcatcgc
//
LOCUS       DASRZF010000291         3579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2970156, whole genome shotgun sequence.
ACCESSION   DASRZF010000291 DASRZF010000000
VERSION     DASRZF010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2970156"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..526
                     /locus_tag="VFG22_12690"
     CDS             179..526
                     /locus_tag="VFG22_12690"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET6417147.1"
                     /translation="MISKIILVVLAVIVGFVLYQRYSSVRVSGSDARSMVAGGAMLVD
                     VRSPAEFAGGHIEGAKSIPIQELSRRMDELGDKDGQIVVYCQSGGRSAMAKRLLESNG
                     FTKVHDMGGIGRW"
     gene            532..738
                     /locus_tag="VFG22_12695"
     CDS             532..738
                     /locus_tag="VFG22_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417148.1"
                     /translation="MAASYICADCDHEFVPEEAGDDPRCPKCSRTEAVEPADTSALQS
                     DIWRQWLVVFALLFIAGIAYVIFE"
     gene            735..1430
                     /locus_tag="VFG22_12700"
     CDS             735..1430
                     /locus_tag="VFG22_12700"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HET6417149.1"
                     /translation="MTIQVSYVSDILCVWAYVAEVRLDELRKEYGSSVELEYRFIPIF
                     GATRHRIGEGWKKRGGYAGFAKHVRDVAADFPHVTVHERAWSEVAPTTSAAAHEILCA
                     ARLLESEGRIDSARREELGGRTQFEEAIWRVRSAFFAEARDISDKQVLREILSEAGLP
                     SAAIDAKLQSGEGMAELCRDIELRDEHKIEGSPTYYLNQGRQKLYGNVGYRVVSANLR
                     ELLERPGHQASWC"
     gene            complement(1462..1614)
                     /locus_tag="VFG22_12705"
     CDS             complement(1462..1614)
                     /locus_tag="VFG22_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417150.1"
                     /translation="MDTTLLVVIMSIVALHVAALAGGGLALGIMRLLGHRPASLNEAE
                     GRDSIH"
     gene            complement(1835..3268)
                     /locus_tag="VFG22_12710"
     CDS             complement(1835..3268)
                     /locus_tag="VFG22_12710"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET6417151.1"
                     /translation="MTLIVDSGARLAPAKPIPRHKGHWLWGGTFDFQKNPAEFLLSAH
                     ERYGDVFVTRVLTGQSYFIRDPEVVNAINVTHAKHIYKPKVVKQMWKPFLGNGLVPND
                     GESWKRQHKLIMPGFHKMRVDAYAPTMAAYTRDMVRGWKSGEQRDFRKEMVELTLRVV
                     AKTLFDTDLAHDSETVHQAIEDISEALIDHAQTPIPTPRWWPSERNKRMVEALDAMDD
                     VIERLVTARRKTREDRGDLMSHIVFAADEQGGMSDKQLRDELMTLIFAGHETTAHTLT
                     WAWYLLATNRDALSRAQREIDDSVGDRPIGVDDLSALPYLEMCLKESLRRLPAVWIFA
                     REAQTDLRLGDYFFPKGSILAISPLAIGRNAKYFDEPEAYRPERWTREFERSLPKGAY
                     VPFAAGPRVCLGKQFAMMEMRIILGTLIQNVDINVLGGFEPDLRPELSLNPGERGMQM
                     RVRFRDGAPVRSGDSDRSPASVDTHAA"
     gene            complement(3358..>3579)
                     /locus_tag="VFG22_12715"
     CDS             complement(3358..>3579)
                     /locus_tag="VFG22_12715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417152.1"
                     /translation="ANDFPRRAHAVLTERPDGTYLVSVRAPLENKQGADDLCRQFPTG
                     GGRAAAAGINALPQHQLDSFVDALARTYP"
BASE COUNT          687 a         1070 c         1120 g          702 t
ORIGIN      
        1 cttcgatgac ctcgagccca cgcggcaaga tcagttcgat caccatgggg aatggatcga
       61 accgggattc ttggcgcgga gcatgaggaa accgtacgcg atccatcgcc ttcccgcatc
      121 tgcgctgagg aatttccgag ctctggcgcg ctccgtgggg gggactatcc tttagcgcat
      181 gatttcgaag attattctcg tcgtcttagc cgtcatcgtg gggttcgtcc tttaccagcg
      241 ctacagctca gtccgggtct ctggaagtga cgcgcggagc atggtggcag ggggcgccat
      301 gctggtcgac gttcggagcc ccgcggagtt cgccggcggc cacatcgaag gagcaaagag
      361 catcccaatc caagagctct ctcgtcgaat ggacgagctc ggcgataaag acggccaaat
      421 cgtcgtctac tgccagagcg gtggacgaag cgcgatggcc aagcggctcc tcgaaagcaa
      481 tggcttcacc aaggtccacg acatgggcgg cataggtcga tggtgagcgc gatggctgcg
      541 agttacatct gtgccgattg cgaccacgag ttcgttccgg aagaggccgg agacgatcca
      601 cgctgcccca agtgcagccg caccgaagcc gtggagccgg cggatacctc cgcgcttcag
      661 agcgatatct ggcgtcagtg gctcgtggtg ttcgctctgc tcttcattgc gggaattgcc
      721 tacgtgatat tcgaatgacc atccaggttt catacgtttc ggacatcctc tgtgtgtggg
      781 cctacgtcgc cgaggtccgg ctcgacgagc tacgcaaaga gtacggcagc tcggtcgagc
      841 tcgaataccg cttcatcccg atcttcggcg caacccggca ccgaatcggc gagggctgga
      901 agaagcgcgg cggctacgcc ggctttgcga agcacgtccg ggatgttgct gcagacttcc
      961 ctcacgtgac cgtccatgag cgcgcctggt cggaagttgc gcctacgacg tcggcggccg
     1021 cccacgagat cttgtgcgca gcccggctct tggagagcga aggccgcatc gattcggcgc
     1081 gccgcgagga gctgggcgga cgcacgcaat tcgaagaggc gatctggcgg gtccgctcgg
     1141 ccttcttcgc agaggcgcgt gacatctccg acaagcaggt gctgcgagag atcctcagcg
     1201 aggctgggct tccaagcgca gcgatcgacg ccaagcttca atctggcgag gggatggccg
     1261 agctctgccg cgacatcgag ctccgggacg agcacaagat cgagggaagc cccacgtact
     1321 acctcaacca aggccgtcaa aagctctatg gcaacgtcgg ctacagagtc gtctcagcga
     1381 atctgcgcga gctgctcgag cggcccggcc atcaagcctc ctggtgctga gcgggggcta
     1441 gccccgcgca gccgcctcga ctcaatggat cgagtcgcgt ccctcggctt cattgaggct
     1501 ggccgggcga tgaccgagca ggcgcatgat gccgagcgcc aggcctccac ccgcgagggc
     1561 ggcgacatga agcgcgacga tggacatgat gacgacgagt agtgttgtat ccatgggaaa
     1621 cccccaatcg gttttgcggg ctgttcgctg gggctccggc ctgcattcct taggctctat
     1681 cctgggcacg acgacgcact gcgtcaaggc ttatggctgc aattttacga tgtcaccatc
     1741 ggaaaaggct gaaacgtgag taattcagcg gctcagtccc agaagatcat cctggaatcg
     1801 gcgtatcaaa cgcggtgctg acctaggaaa agcctcaagc agcatgggtg tcgacggacg
     1861 ccggactgcg atcgctatct ccggagcgaa ccggcgcccc gtcccggaag cgcacccgca
     1921 tttgcatgcc tcgctcgccg ggattcagcg agagctcggg gcgcaagtcc ggctcgaagc
     1981 ctccgaggac gttgatgtcg acgttctgga tgagcgtgcc gaggatgatg cgcatctcca
     2041 tcatcgcgaa ctgcttgccc aggcaaacac gcggtcccgc ggcgaaggga acgtaagcgc
     2101 ccttgggaag gcttcgctcg aactcccgcg tccagcgctc aggccgataa gcttcgggct
     2161 cgtcgaagta cttggcgttg cgcccaattg cgagcggact gatcgcgagg atcgagccct
     2221 tcggaaagaa gtagtcgccg agacgcaggt ccgtttgtgc ctctcgggca aagatccaga
     2281 cggcgggaag cctgcgaagc gactccttga ggcacatctc gaggtacgga agcgcgctga
     2341 ggtcatcgac tccaatcggt ctgtcaccga cgctgtcatc gatctcgcgc tgcgctctgc
     2401 tgagggcgtc cctgttcgtt gcaagtagat accaggccca agtgagtgtg tgcgccgtgg
     2461 tttcatgccc ggcaaagatc agtgtcatca actcgtctcg gagctgtttg tcggacattc
     2521 cgccctgctc gtccgccgcg aagacgatat gcgacatgag gtcgcctcgg tcttctcgag
     2581 tcttgcggcg tgcggtgacg agcctttcga tcacgtcatc catggcatcg agcgcctcga
     2641 ccatccgctt gttgcgctcc gacggccacc aacggggggt tggaatcggt gtctgggcgt
     2701 gatcgatcag cgcttcgctg atgtcttcga tcgcttgatg aaccgtctca gaatcgtgag
     2761 cgaggtccgt gtcgaacagg gtcttcgcga cgacacgaag cgtgagctcc accatttctt
     2821 ttcggaaatc gcgttgctca ccgctcttcc agccgcgaac catatcgcgc gtgtacgcag
     2881 ccatggtcgg cgcgtatgcg tcgacacgca tcttgtggaa ccccggcatg atcaacttgt
     2941 gctgtcgctt ccacgactca ccgtcgtttg gcacgagccc atttccgaga aagggcttcc
     3001 acatctgctt cactaccttg ggcttgtaga tgtgctttgc gtgggtgacg ttgatcgcgt
     3061 tgacgacctc cgggtcgcgg atgaagtagg actgaccggt gagcacgcgc gtcacgaaga
     3121 cgtccccgta tcgctcatgt gcggagagca ggaactccgc cggattcttc tggaagtcga
     3181 aggttccgcc ccagagccag tgtcccttgt ggcgtggaat cggcttcgcg ggcgcgaggc
     3241 gggcaccaga atcaacgatc aaagtcattt acttaacgta aagtaaactt atctgatacg
     3301 acccgcaagt taacaagttg ttttcttaag ccctcggcga gcgttgacac tcgagcgcta
     3361 gggataagtg cgagccagcg catcgacgaa ggagtcgagc tgatgctgag gcagtgcgtt
     3421 gatgccagcc gctgcggccc tcccgccacc ggtagggaac tgcctgcaga gatcgtcagc
     3481 gccctgcttg ttctcgagcg gagcgcgaac actcaccaag tacgtgccat cgggccgttc
     3541 ggtgagcacc gcatgcgccc gtctcgggaa atcgttggc
//
LOCUS       DASRZF010000292        12811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4347916, whole genome shotgun sequence.
ACCESSION   DASRZF010000292 DASRZF010000000
VERSION     DASRZF010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 12811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12811
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4347916"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VFG22_12720"
     CDS             complement(<1..737)
                     /locus_tag="VFG22_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HET6417153.1"
                     /translation="MSKNELKGGRLSSFPIADLKAGFLVFLIALPLCLGIAMASGFPP
                     ISGVLTAIIGGVFVSFLGSARLTIKGPAAGLIVIALGAVQELGQGDPMLGYRRALAVG
                     VVAACVQIALALLRAASIGVAMSPSVVHGMLAAIGVIIISKQSHTLIGVAPEAKEPLA
                     LLAEIPHSIAHANPEILALGLVSLVILFGWPMIRRAWAKAIPAPIVVLAFAIPVSLAL
                     DLDHSHAYQFLSHQFHVGPEYLVRLPGS"
     gene            complement(1289..1942)
                     /locus_tag="VFG22_12725"
     CDS             complement(1289..1942)
                     /locus_tag="VFG22_12725"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET6417154.1"
                     /translation="MSNAYKSEVPRTYRVQELVRDLMSISARAPALVAIWLGGRLPPA
                     LREQVVVAVARVNACSMCEHAHTRMALEAGVSNAELAALENMDEGAFDRRTWLAIAHA
                     RERTKADFVRPPADQAREVLIQELGAQTYRDVEDVARVMTVANRIANTLNALSDRAHG
                     NPVPGSRLLDELVINILFLPAAWLGTLVAAARQRKSPFAVWRQARQPRVAPRPGSRD"
     gene            complement(1945..2811)
                     /locus_tag="VFG22_12730"
     CDS             complement(1945..2811)
                     /locus_tag="VFG22_12730"
                     /inference="COORDINATES: protein motif:HMM:NF039714.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6279 family lipoprotein"
                     /protein_id="HET6417155.1"
                     /translation="MHSVASWPCRRRALRASLALCLVCVCACTGVLQNLGARWVTNKI
                     SGVFALDEAQQNATRAAVDRTIAVAPEILGPRLDFLVATVDRAIFKGMNEESILLIEK
                     QVDILMDKALGWIIDEAAPILATLSDEQIAHAERELDNRLQGTRDELNAPEDERTSER
                     QDKFVEAIENWTGRLSDDQERAIRLHVAAMPDEAADRLRADENRLGLIADGLRRHPGT
                     PAIRNLLWDAWLQREDWGPGTRPPEARRADGRKTLLFVYQLLDAEQRDQMSERLHEMH
                     RTAKRLMGMAEG"
     gene            complement(2811..3074)
                     /locus_tag="VFG22_12735"
     CDS             complement(2811..3074)
                     /locus_tag="VFG22_12735"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET6417156.1"
                     /translation="MKERRVAGILCSEVLALLSDYVDGELDSVAVENVESHLLGCPNC
                     ERFGRNFGSMVVSLREELARSPEMQLDVMARLLARLRESDTRS"
     gene            complement(3074..3682)
                     /locus_tag="VFG22_12740"
     CDS             complement(3074..3682)
                     /locus_tag="VFG22_12740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET6417157.1"
                     /translation="MFDPSADDDPRTDQELIALSAAGDRSAFDRLVERHAAAVLRLTN
                     LVTGDPMTAEDALQQTFLAAYRNAASFRSEASVRTWLLAIARNAALRHRSKRGREDIV
                     AESLMQLGLDAGWGAENPEALAIAAEREELLRRAMGDLCTEDQEVLVLRDVEGLSGLE
                     AAEVLGIGERALKSRLHRARLRLAGSLRAALVPDSPEGRGGR"
     gene            4088..4648
                     /locus_tag="VFG22_12745"
     CDS             4088..4648
                     /locus_tag="VFG22_12745"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HET6417158.1"
                     /translation="MKSVFNKAVFLTALSLLGPGLAASGHAQVYYSVRGLLSEQFHQS
                     QVVDFQRVQISAEHRDAIEHRLGQKLERQEYIFYVARTADQVDGYALFDREIGQHEYI
                     DFATFFDDAGRVTRVEVVAYREPYGEGIRSKRFRKQFVGKEATSGFRPGHDIDVISGA
                     TLSARAMAKAVRRATLLLHDTLLTAS"
     gene            4656..5204
                     /locus_tag="VFG22_12750"
     CDS             4656..5204
                     /locus_tag="VFG22_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417159.1"
                     /translation="MQDFTRPSGRLRALPLGARAVYTVFLVFTLLGLALTLWLTHDMV
                     GIDLKGVTEYYGGGATPIAEVEVESGPALFLPPGGDDLAAFEPMPRRKLLEITHFHLF
                     SMPVYLLILSHMYMLSRSRKTAKAAWIGVGSFGTLLHIAAPWLVAQASVGALASYAIS
                     GAMLFISYSVMSVVPLWEMWRR"
     gene            5254..5616
                     /locus_tag="VFG22_12755"
     CDS             5254..5616
                     /locus_tag="VFG22_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417160.1"
                     /translation="MAEEGKSGKRVVGVLLGLVLAVGVVAWLGAMRLEKGSHPGGDAK
                     AEIASVLSEGVLYEARAGDPGIVLAKDVGSGKELWRSELGSITSKPTLAVNEDVIEVQ
                     IAGTPWMTLNKATGEPLE"
     gene            5613..6020
                     /locus_tag="VFG22_12760"
     CDS             5613..6020
                     /locus_tag="VFG22_12760"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HET6417161.1"
                     /translation="MTSGSTPEVVEGGCHCGAVRYRVTVRSREAVECNCSICAKKGFL
                     HLIVPENDFELLRGADAITTYTFGTHTAKHHFCGHCGIHSYYRPRSHPDEVDVNVRCL
                     DGMNRDDFRFTSFDGQNWEASVEKLRELHRNRQ"
     gene            complement(6014..7447)
                     /locus_tag="VFG22_12765"
     CDS             complement(6014..7447)
                     /locus_tag="VFG22_12765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET6417162.1"
                     /translation="MRLHATSSRTATSTARSSGSTVPFAWHPASLPFSARVSEPRRRR
                     ILGLSLPIIGGMLSQNVLNLVDTAMVGTLGDAALAGVGLGGFANFLFSAFILGLSAGV
                     QAMAARRVGEGRLGETAIPLNGGLLLALAIAVPWSALLIAFAPEYFPVLTSDAAVADQ
                     GVPYLQARLFAMFAMGMNFAFRGYWNAVDKSILYMRTLISMHVVNIFLNWVLIFGNLG
                     APELGAAGAGVASAIATVFGTASYFLLGRGYARDAGFMRGLPERATMATIIRLAAPAG
                     LQQFFFAAGMTVFLMLVARMGTPELAATKVIIDLMLVGILPGIGFGLAAASLAGQALG
                     RGEPADAMQWGWDVVRMAVLVVGGLSIPAILAPEWILSGFIHEPSTLAIARNPLRLVA
                     SFLFIDAVGMVMMNALMGAGDVRRVMVISTGFQWLIFLPLVYLLGPVLGFGLVVVFAL
                     QAIYRGFQALTFALMWKRGRWQSIELH"
     gene            complement(7359..8123)
                     /locus_tag="VFG22_12770"
     CDS             complement(7359..8123)
                     /locus_tag="VFG22_12770"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6417163.1"
                     /translation="MRIDGSVALVTGGASGLGEATVRQFVSRGGSAVIVDLNEERGNA
                     LATELGAAATFVRADVSNPEQVQAAVDRATAMGTLRAAISCAGIGIAMRTIGRDGTPH
                     DLGAFQKIISVNLFGTFNILRLAASAMAKNEPQDGERGVVVNTASVAAFDGQIGQVAY
                     SASKGGIVGLTLPAARDLAKSQIRVVTIAPGTFDTPLLASLGEEIRAALAATIPHPSR
                     LGRPDEFGALACHIVENSYLNGEVIRLDGAIRMAPR"
     gene            8235..9662
                     /locus_tag="VFG22_12775"
     CDS             8235..9662
                     /locus_tag="VFG22_12775"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HET6417164.1"
                     /translation="MLFNSLDYILFLGVAVAGFWLLARHAQLRIIFIFAASCLFYMAW
                     HPAYIVLIIGSTLVDYVVGLRIHATENEIARKRWLLVSVVSNLGLLGVFKYFNFASQA
                     TADVLALFFGIEIPNPPFLDVLLPVGISFYTFQTLSYTIDIYRRKLEPTRNFFQFALF
                     VTYFPQLVAGPIVRASQFLPQLQRKITLRDEQVTQGLFLIATGMVKKVAIADYLSVNL
                     VDRVFDQPELYSAAEVVVALYGFTMQIYCDFSGYTDVARGSAKLMGLELPENFDRPYQ
                     SASPAEFWRRWHMTLSTWLRDYLYYPLGGSHVGPLRAYWNLWLTMFLIGIWHGASWTF
                     VFYAILQSLAMVIHRFFYRRSGRNRDTVDRWQTHVFKVFWALQFVVFSRILFRATSLQ
                     NAADVAYQLGSGTFSVAQISVGIWAMLFFTFAAHFAPKRWFESIELSFKSMPAPAQGL
                     TLAVLGFILSAVATSEVVPYIYFQF"
     gene            9662..11275
                     /locus_tag="VFG22_12780"
     CDS             9662..11275
                     /locus_tag="VFG22_12780"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HET6417165.1"
                     /translation="MLKRIYSQFTPLQHLFVVTVFMFAACVLTYVVPAMASLRPWIPG
                     EEGIPIVRLFKLWGEVPAFAGHGGSYRATASSESDTERLAETVGETVAANLGSKPFVA
                     RPHKPEEGFRIDPEEYAGISVRIEDPTGRGMRAFYEALEKTARGQKGAITRIGHYGDS
                     SIATDLITSTLRENLQGRFGDAGHGFVLAAKGYIPYKHRGVRQSSNPDAWEVREVVRR
                     QDRHGRYGYGGVQSRALGGAWARVGTAKRGGTGTNASRFDIYYQEQPRGGKFSVRLDG
                     EEIETISARADEIGDVVYSIEAPDGPHFIELRHMRGGPVSFYGVAVERDGPGVVYDSL
                     GLVGARANRLLNFDEAHIKRQLELRGADLIVLGFGGNEASDDRTEDFFYEDYVGVLQR
                     MRQGREDLSCLVFAPLDQARRTRGRIRTLPTIPLIVAAQRRAAIDSGCAFYDTWEAMG
                     GEDAMRSWYKAQPRLAMGDFRHATPAGYEIIGNMFYKAILAGFADYLAAEPSTTPASS
                     TPADTDAGSELPDSGMPSSNAASRTSPKR"
     gene            complement(11320..12513)
                     /locus_tag="VFG22_12785"
                     /pseudo
     CDS             complement(11320..12513)
                     /locus_tag="VFG22_12785"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
BASE COUNT         2523 a         4042 c         3870 g         2376 t
ORIGIN      
        1 gagcccggca accgaacgag atactcgggt ccgacatgga actgatgcga cagaaattga
       61 taggcgtggg agtgatccag atccaaggcg aggctcaccg ggatggcaaa tgccaaaacg
      121 acgatcggcg cagggatggc cttcgcccag gctcgccgga tcatcggcca gccaaataga
      181 atgacgagag agaccaggcc gagggctagg atttccggat tggcgtgcgc gatgctgtga
      241 gggatttcag caagcagcgc gagaggctcc ttcgcttcgg gagccacgcc gatgagcgtg
      301 tggctctgct tcgaaatgat gatcacgcca atcgcggcga gcattccgtg aaccaccgaa
      361 ggcgacatcg cgacaccgat ggatgcggct cgaagaagag cgagcgcaat ctggacgcag
      421 gccgcgacaa cgccgaccgc caaggcgcgg cgatagccca gcatcgggtc cccctgcccc
      481 agctcctgaa ccgccccgag cgcgatcacg atcagccccg ctgcggggcc cttgatcgtc
      541 agtcgtgcgc ttcctaggaa gctgacgaac acacccccga tgatcgccgt caggactccg
      601 gagatcggag gaaatccact ggccatcgcg attcccagac agagggggag cgcgatcagg
      661 aagaccagga acccggcctt gagatccgca atggggaacg agctcaatcg cccgcccttc
      721 aattcatttt tcgacatgtc actccgcctt cgcgttcaaa ataccgcgtt gccccgacgc
      781 tgggggcatt cacacacagt gaagacgaaa ccccaagaag ggcttcgtca ggccagaggg
      841 gggccgcgcg cggtttggga gcgagcgaga tcgctgctgg agggtcgccg agcacaggca
      901 tcggtaaccg tcgaagcgcc agaaatccga atcgggggaa gactcagttg ttggaaactg
      961 gggtcatggt cctcggggga gtcgatggcc ccgtcagcac aggctccgac ccgctcgggc
     1021 gccggctctc gtatgagctt cgactgagcc gccggcgggc tcagggtggc attcccacgg
     1081 gcggacatat cccctgacgt aaccaaaaag acaggcaggg cgaggacgag cagggacatc
     1141 aaggcggcgc tctgcgccag ccgatgcaat gcatcccccc gtacctttgc tcttcctaag
     1201 tccattcgaa gcagcttaac cccgcaaccg accaagtatt ccgccaaaga atggcaagtg
     1261 caagatgggc aacccgcctg ctccgatttc aatcccgaga gccgggtcgc ggcgcaaccc
     1321 tgggctgacg tgcttgtcgc caaaccgcaa agggcgactt ccgctggcgc gcggccgcca
     1381 cgagcgtgcc caaccacgca gctggcagga acaggatgtt gatgaccagc tcgtcgagaa
     1441 gtcggctacc cggcaccggg ttcccatggg cacgatccga aagggcgttg agcgtattgg
     1501 caatacgatt cgcaacagtc atgacacgcg ccacgtcttc cacatcgcga tacgtctgag
     1561 ccccgagctc ttggatcaac acctcccgag cctgatccgc gggaggtcga acgaaatcgg
     1621 ccttggtgcg ctcgcgtgca tgggcaatcg ctagccaggt gcgccgatcg aacgcaccct
     1681 cgtccatgtt ttcgagggcg gccagctcgg cattggatac gccggcctcg agggccattc
     1741 gcgtatgcgc gtgctcgcac atgctgcagg cgttgactct ggccaccgcg acgacgacct
     1801 gctcgcgcaa ggccgggggc agtcgcccgc ccagccaaat cgctaccagc gccggtgcgc
     1861 gtgcggagat cgacatcagg tctcgaacaa gctcttgcac tctgtaggtg cgaggaacct
     1921 cagatttgta tgcgttcgac acgattagcc ctccgccata cccatcagac gcttcgcggt
     1981 gcggtgcatc tcgtgaaggc gctcgctcat ttgatccctc tgctcggcgt cgagtagctg
     2041 atagacgaac aagagggtct tgcgaccgtc ggctctgcgc gcctcgggtg ggcgcgtacc
     2101 ggggccccag tcctcacgct gaagccacgc gtcccagagc aggtttcgaa tcgcgggcgt
     2161 ccccgggtgc cgacgcagtc cgtcggcgat cagtcccagt cggttttcgt cagctctcag
     2221 ccggtcggcc gcctcgtcgg gcatggcggc cacatgcagg cgaatcgctc tctcttgatc
     2281 gtcgctcagc cgacccgtcc agttctcgat cgcctcgacg aatttgtctt gtcgctcgct
     2341 ggttcgctca tcctcagggg cgttgagctc gtcgcgcgtg ccttgaaggc gattgtcgag
     2401 ctcacgctcg gcgtgcgcga tttgttcatc gctcagggtt gccaagatcg gcgcggcttc
     2461 gtcgatgatc cagccaagcg ccttgtccat gaggatgtct acctgcttct cgatcagcag
     2521 aatgctctct tcgttcatgc ctttgaagat ggcgcggtcg accgtggcga cgagaaaatc
     2581 gagccggggt cccagaatct cgggagcgac cgcaatcgtg cggtcgacgg cagcccgggt
     2641 tgcattctgc tgagcttcgt cgagagcgaa gacacccgaa atcttgttcg tgacccagcg
     2701 tgcaccgagg ttctggagaa ccccggtaca ggcacacacg cagaccaggc acagagccaa
     2761 cgacgcgcgg agcgctcgac ggcggcaggg ccagctcgca accgaatgca tcaagacctc
     2821 gtgtcggact cgcgtagccg cgccagcaat cgcgccatga catcgagctg catctccgga
     2881 ctccgtgcca actcttcgcg aagcgatacc accatggacc cgaagttccg tccgaagcgc
     2941 tcgcagttgg ggcaaccgag aaggtggctc tccacgttct cgaccgctac cgagtcgagc
     3001 tcaccgtcca cgtagtcgct caagagcgcc agcacttccg agcaaaggat tcccgccact
     3061 ctccgctctt tcatcaccga cctcctcgtc cctcgggaga atccggcacc aacgcggccc
     3121 tgagcgagcc cgccaatcga agtcgcgccc gatgcagtcg gctcttcaat gctcgctctc
     3181 cgataccgag gacctcggcg gcctcgagac cggagagacc ctcgacgtcc cgcaagacga
     3241 ggacctcctg atcctccgtg caaaggtcac ccatcgcccg tcgaagtagc tcctctcgct
     3301 cggcggcgat cgctagggcc tcggggttct cagcgcccca gcctgcatcg aggccgagct
     3361 gcatcagtga ttctgccacg atgtcctcgc gaccgcgctt cgatcgatgg cgaagggccg
     3421 cgttgcgcgc gatggcgagc agccacgtgc gaaccgatgc ctcggaacga aagcttgcag
     3481 catttcgata tgccgcgaga aacgtctgct gcagggcatc ttccgcagtc atgggatcac
     3541 cggtcaccag gttcgtcagt ctgaggaccg ccgcagcatg gcgctcgacc agacggtcga
     3601 acgcgctgcg gtccccagca gcgctgagag cgatcaactc ttggtcggtc cgtgggtcgt
     3661 cgtctgctga tggatcgaac atgtcgagat cccgaacagc cgatagaccg ggcagctgcc
     3721 gactaggccc gttaccagcg gtaccagacc gacccaaccc catgctgtct gaggaccgat
     3781 ccataccagt gataagagac cgagcccaag cactactcgg atcacgcgat cgacagcgtg
     3841 ttcgtttcgg ggaaaaatgg cactcatgga aaagctcctt ttgcaggccc agacccgagc
     3901 gatcgtttgt gaccgctcag ccgagccgct gcttctatga gcctgttggc ggccaaaagg
     3961 attcaggagg gcggagagtt ttcctccatt tgagacgcca accgacctcg gggctcggaa
     4021 atcgcaaatt ttgcgtgtaa tttccgcttg cggggattct gggctgccct tatcgttgaa
     4081 aacgaagatg aaaagcgttt tcaataaagc ggtctttctg acggcgcttt cgctcctggg
     4141 gccgggcttg gcggccagcg gacacgcaca ggtgtactac agcgtgcgcg gtctactctc
     4201 ggaacagttc caccaatcgc aggtggtcga cttccagcgc gttcagatca gcgcagagca
     4261 tcgcgatgcc atcgagcatc ggctcgggca gaagctcgag aggcaagagt atatcttcta
     4321 cgtcgcacgc accgccgacc aggtggatgg ctacgctctt ttcgatcgag agatcggtca
     4381 acacgagtac atcgacttcg ctactttctt cgatgacgcg gggagagtga cacgggtcga
     4441 ggtcgtcgcg taccgcgagc catatggcga gggaattcgt tccaaacgct tccgcaagca
     4501 attcgtaggc aaagaggcca cgagcggctt caggcccgga catgacatcg acgtgatctc
     4561 tggcgccaca ctttctgccc gcgcgatggc aaaggccgtt aggcgcgcga ctttgttgct
     4621 gcacgacact ctgctcaccg cgtcttagcc aagcgatgca agatttcact cgaccttctg
     4681 gacggctccg cgccctaccc ctcggcgcac gggccgtcta cacggtgttt ctcgtcttca
     4741 cgctcctcgg cttggcgctc acattgtggc tcacgcacga catggtcggc atcgatctga
     4801 agggcgtcac cgaatactac ggaggcggcg ccactccgat tgcagaggtg gaggtcgagt
     4861 cgggccctgc gctgttcctg cctcccggcg gcgatgacct cgccgcgttc gaaccgatgc
     4921 cgcgccggaa gcttctcgag atcacccact ttcacctgtt ctcgatgcct gtgtatctgc
     4981 tgattctcag ccacatgtac atgctctcgc gctcgcgaaa gacggctaag gcggcctgga
     5041 tcggcgtggg ctcgtttggg accttgttac acatcgccgc gccctggctc gttgcgcagg
     5101 cttctgtggg cgcccttgcg agctatgcga tttccggggc gatgttgttc atcagctact
     5161 cggtgatgag cgtcgttccg ctttgggaaa tgtggcggcg ctgagcgcgc tacgctcgtg
     5221 acctcggcat gtccctcatg ctaaggagca gtcgtggctg aagagggcaa atcaggcaaa
     5281 cgcgtcgtcg gggtcctgct ggggctggtc ctcgcagttg gcgtcgtcgc ttggttgggg
     5341 gcgatgcgac tcgagaaggg aagtcacccc ggaggcgacg ccaaggcgga aatcgcttcg
     5401 gtgctgagcg agggagtgct ctacgaagcc cgggcaggcg atccgggaat cgtcctcgca
     5461 aaggacgttg ggtccgggaa ggagctttgg cggagcgagc tcggctcgat cacgagcaaa
     5521 ccgaccctcg cagttaatga agacgttatc gaagtgcaaa tcgctgggac cccttggatg
     5581 actctgaaca aggcgactgg cgagcctttg gaatgacgag cgggtctacg ccggaagtcg
     5641 tcgaaggcgg ctgccattgt ggagcggttc gttatcgagt gaccgtgcgc tcgcgcgagg
     5701 cagtcgagtg caactgcagc atctgtgcga agaagggatt tcttcatctg atcgtgccgg
     5761 agaatgactt cgagctcctt cgaggcgccg atgcgatcac gacgtacaca ttcggaacgc
     5821 acacggcgaa acaccatttt tgtggtcact gcggcattca ctcgtattac aggcctcgct
     5881 cccaccccga tgaggtagat gtcaacgtgc gttgcctcga tggcatgaat cgggacgact
     5941 tccggtttac gagcttcgac gggcaaaact gggaagccag cgtcgaaaag ctgcgcgagc
     6001 tccaccgcaa ccgtcagtga agctcgatgg actgccaacg tcctctcttc cacatcaggg
     6061 cgaatgtgag tgcctggaac ccgcggtaga tcgcttgaag ggcgaatacg accacgaggc
     6121 cgaagcccag aacagggccg agcaagtaga ccagcggcag gaagatcagc cactgaaaac
     6181 ctgtggaaat gaccatcacg cgcctgacgt ctcccgcgcc catcaaagcg ttcatcatca
     6241 ccatgccgac agcatcgatg aagagaaagc tagccaccaa gcgaagcggg ttccttgcga
     6301 tggccaaagt ggagggctcg tggatgaagc cgctcaggat ccactccgga gccaagatcg
     6361 ccggaatgct caagcctccc acgaccaata ccgccatcct gacgacgtcc cagccccact
     6421 gcatggcatc ggccggctcg ccgcgaccga gcgcctgtcc cgccagcgat gccgctgcta
     6481 gcccaaaccc gatacccggc aagatgccga cgagcatgag gtcgatgatc accttggtcg
     6541 cagccagctc gggagttccc attcgcgcga cgagcatgag aaagacggtc atgccggccg
     6601 cgaagaagaa ctgctgaagc cccgcaggag cggcgaggcg aatgatcgtc gccatggtgg
     6661 cgcgctcggg aagcccgcgc atgaagcccg cgtctcgcgc atatccccgc ccgagaagga
     6721 agtacgaggc cgttccaaac accgtcgcga tcgccgaagc cacgccagca cctgcggccc
     6781 cgagctcggg cgccccgagg ttgccgaaga tcagcaccca gttgagaaag atgttcacga
     6841 cgtgcatcga gatcaaggtg cgcatgtaaa ggatcgattt atccactgcg ttccaatagc
     6901 cgcggaacgc aaagttcatg cccatggcga acatcgcgaa caaccgcgct tgaaggtatg
     6961 gaacaccctg gtcggcgacc gccgcgtcgc tcgtgagcac tgggaagtac tcgggagcga
     7021 acgcgatgag caaggccgac cagggcacgg cgatcgcgag agcgagcagg agaccaccat
     7081 tgagcgggat ggctgtctcg ccgaggcggc cctcccccac acgacgcgct gccatcgctt
     7141 ggacgccggc tgaaagcccc aatatgaagg cagagaatag gaagttcgcg aaacctccga
     7201 ggccgactcc tgccagcgcc gcgtcgccaa gcgttccgac catcgccgtg tcgacgaggt
     7261 tgagcacgtt ctgagaaagc atccctccga tgatcgggag ggagagcccg agaattcggc
     7321 gccggcgcgg ctccgagact ctcgccgaga agggcaagct agcggggtgc catgcgaatg
     7381 gcaccgtcga gccggatgac ctcgccgttg aggtagctgt tctcgacgat gtggcatgca
     7441 agcgcaccaa attcgtcggg gcgtccaagc cgactcggat gcggtatcgt cgcagccaat
     7501 gctgcgcgta tttcctcgcc tagcgacgca agcaagggag tatcgaacgt gcccggcgcg
     7561 atggtcacga cgcgaatctg ggacttggct agatcgcgcg cggcggggag cgtcaagcca
     7621 acgatgccac ccttggaggc cgagtacgcg acctgcccta tctgtccatc gaatgcagca
     7681 actgaggccg tgttcaccac gacaccgcgt tccccgtcct gaggctcgtt cttcgccatc
     7741 gccgaagccg cgagccggag gatgttgaaa gtgccgaaca gattgacgga gatgatcttc
     7801 tgaaacgctc cgaggtcgtg aggcgtgcca tctctgccga ttgtgcgcat cgcgatgccg
     7861 attcccgcgc agctaattgc ggcccgaagc gtgcccatcg cggtcgcccg gtcgacggca
     7921 gcctgaactt gctccgggtt gctcacgtca gcacggacga aggtcgctgc agcaccgagc
     7981 tcggtcgcta gggcgttccc gcgctcctcg ttgaggtcga cgatgactgc gctcccccct
     8041 cggctcacga actggcgtac cgtcgcctca cccaagccag aagctccccc cgtcacaagt
     8101 gctaccgaac catcaatcct catcgacacc tccgaccgca gatgcttggt ctaagcggcc
     8161 tcccggtcaa gcagctagcg tgcaggccca gctgcggcta aaaccgggcg cgttcacgct
     8221 gggtaagggg accagtgctc ttcaacagcc tcgattacat tctgttcctg ggcgtcgctg
     8281 tcgcgggatt ctggctgttg gcgcgccacg cccaactgcg gatcatcttc atcttcgcgg
     8341 cgtcatgcct gttctacatg gcgtggcacc cggcctacat cgttttgatc atcggatcga
     8401 ccctggtcga ctacgtggtg ggccttcgca tccatgcaac tgagaacgag atcgcgcgca
     8461 agcgctggtt gctcgtcagt gtcgtttcga acctcgggct gctgggcgtt ttcaagtact
     8521 tcaacttcgc ttcgcaggcg acggccgacg tactcgcgct cttcttcggc atcgaaatcc
     8581 cgaacccgcc gtttctagac gtccttctgc ccgtcggcat ctcgttctac acgttccaga
     8641 cgctcagcta cacgatcgac atctaccgac gcaagctcga gcccacgaga aacttctttc
     8701 agttcgcgct cttcgtcacg tatttcccac aactggtcgc tggtccgatt gtgcgcgcgt
     8761 cgcaattttt accgcagctg cagcgcaaga tcacgcttcg cgacgagcag gtcactcagg
     8821 ggctcttcct catcgcgact ggcatggtga agaaggtcgc catcgcggac tatctttcgg
     8881 tcaatctggt cgaccgcgtg tttgaccaac ccgagctcta cagcgccgca gaagtcgtcg
     8941 tggcgctcta tggcttcacg atgcagatct actgcgactt ctcaggctac accgacgtcg
     9001 cccgaggatc ggccaagctg atgggtctcg agctgcccga gaacttcgac cggccctatc
     9061 aatcggcgag tcctgccgaa ttctggcggc gctggcacat gaccctttcg acctggctgc
     9121 gcgactactt gtactacccg ctcggcggat ctcacgttgg ccctctccgg gcctactgga
     9181 atctctggct cacgatgttc ttgatcggca tctggcacgg ggcatcctgg acgttcgtct
     9241 tctatgcaat tcttcaatcg ctggcgatgg tcatccaccg cttcttctat cgcaggtcgg
     9301 ggcggaatcg cgatacggtc gatcgctggc agacgcatgt tttcaaggtg ttctgggccc
     9361 ttcagttcgt cgtcttctcg cgcatcctat tccgcgcgac gagcctgcag aacgccgccg
     9421 atgtcgcata ccagctcggc tctggaacct tttccgtcgc tcaaatttcg gttggaatct
     9481 gggccatgtt gttcttcaca ttcgccgcgc acttcgcgcc gaagcgctgg ttcgagtcga
     9541 tcgagctcag tttcaagtcg atgccagcgc cagcgcaggg cctcaccctc gcggtactcg
     9601 gcttcattct gagcgccgtc gcaacgagcg aggtcgtccc ttacatctac ttccagttct
     9661 gatgctcaaa cgaatctact ctcagttcac tcctctgcag cacctcttcg tggtgacggt
     9721 cttcatgttc gcagcgtgcg tgttgacgta cgtggttccc gcgatggcga gtctacgtcc
     9781 ctggataccc ggcgaggagg gcatcccgat cgttcggcta ttcaaactct ggggtgaggt
     9841 ccctgcgttc gccggccacg gcggctcata tcgtgcgacg gcaagcagcg aatcggacac
     9901 ggagcgactt gccgaaaccg tgggcgaaac ggtagctgcg aatctgggaa gcaagccctt
     9961 cgtcgcacgc ccacacaaac ccgaagaggg cttccggatc gacccggaag agtacgcagg
    10021 catctcggta cggatcgagg acccgaccgg aagaggcatg cgcgcattct acgaggcatt
    10081 ggagaaaacg gcgcgtgggc aaaaaggcgc aatcacgagg atcggacact acggggactc
    10141 gagcatcgca accgacttga tcacatctac cttgcgcgaa aacctgcagg ggcgattcgg
    10201 agacgctggc cacgggttcg ttctcgccgc caaggggtac attccgtaca agcaccgggg
    10261 agtccggcag tcttcgaatc cggacgcttg ggaagtgcgc gaggtcgtcc ggcgtcaaga
    10321 tcgccatggc cgctacggct acggaggcgt ccagtcgaga gctctgggag gcgcgtgggc
    10381 gcgcgtgggc actgcgaagc gcggcgggac cggaacgaat gcgtcgagat tcgacatcta
    10441 ctaccaagag cagcccagag gcgggaagtt ctcggttcgc ctggacggtg aggagataga
    10501 gaccatctcg gcaagagccg acgagatcgg cgacgtagtg tattcaatag aggcgcctga
    10561 cggcccacac ttcatcgagc tgcgccacat gcgcggtggt ccggtgagtt tctacggcgt
    10621 cgcggtcgag cgagacggtc caggggtggt ttacgactcc cttggactgg tcggtgcacg
    10681 cgcaaatcgc ttgctcaatt tcgatgaggc gcacatcaag cgtcagctcg agctgcgcgg
    10741 cgccgatctg atcgttctcg gcttcggtgg aaacgaggca agcgatgacc ggacggaaga
    10801 cttcttctac gaagactacg taggtgtgct ccagcgaatg cggcagggcc gcgaggactt
    10861 gagctgtctc gtcttcgctc ccctcgacca agcacgacgt actcgaggac gcattcgtac
    10921 gctgccgacg atcccgctca tcgtcgctgc gcagcggcgg gcggccatcg attcaggctg
    10981 tgccttctac gacacctggg aggcgatggg gggcgaagat gcgatgcgaa gttggtacaa
    11041 ggcgcaaccg cgcctcgcga tgggcgactt ccgtcacgcg accccggcgg gctacgagat
    11101 catcggcaac atgttctaca aggcaatatt ggccggcttc gccgactatc ttgccgccga
    11161 gccgtcgacc acacccgcgt catcgacacc ggcagacaca gacgccggat cggagctacc
    11221 ggactccggc atgccatcga gcaatgccgc gagcaggact tcgcccaagc gctgataccc
    11281 gacccgagtg tagtggatgt ggtcctgcga tgcgtacgca gggtcgttcg cggcccattg
    11341 caccatcgag agcgcaccac cctggaacgc caccaagtcg aagaacccgc atccgtgctc
    11401 tagcgcaacc cggtgttgta cttcgattag ccgcgctgta cggctgcgat cctcgaaaac
    11461 gcgctcttcg acttgtacgg gtcgatccga agggccgatg agaagacaag atgcatcggg
    11521 gacagcgtcc ttcaatcgct tgactaccgc ccgtagatcg ctctcatagt cttcgagcgg
    11581 tgattcatcg ccgctttcgt tggtgccgta agcgagcacg acgaggtccg gttcgcgacg
    11641 acgaatctgc tcgcgaaata cttcctcgtc ccaaaggagc tgataccgcg cccgagagcc
    11701 gttgatgccg agcgtatcga ctacgacgcc tggctggtca cgttcgacgg tgagaccgta
    11761 tatccagacc ggtttcttgc tcagggttcg aatctcgaat cgatgcggcc catctggcac
    11821 acggaaagtc gcgtagccgg tcgagacgtc ttttgcccgt gtcgagatgc gcctcgcctt
    11881 ccggccatcg atcacgacgt ccaggtcgcc ccctcgggga tgcttcatgt agtagagctc
    11941 gaagaagctc gcatcgcggc cgtagtcgct ttcctgcgcg gtgaagaccg cgccgtagga
    12001 tccgagacgc tgactcatca tggtgatgcc agcgagtcct agacgctcca cttcggtatc
    12061 gccgacccgg atgcgctggg tttcccatcg gtcaccgtcg ctctcgatgt tgatgtcgcg
    12121 gtgccggtag gttcgccatg gatgcaccgg aaccaagaaa ccgtgccccg cgtcaccata
    12181 gcgggactgc aactcacggc ggatgtgccc cgtaaacaaa tcacttgcga cgtgcgaagc
    12241 gccaaagaaa agcagtcgcg ccttgccctc tcccgacgcg gcccgctgaa gagctcgatg
    12301 cagagccagc atcgacttgc catcgcggtc ctcgatcgcg atatcgaggc ccaatccatc
    12361 actcggcggc ggctgcagga gcttgggccc catccgttgc cccgcgcgaa ccgcatcgag
    12421 cacacccgtt tcgtcgatcg ctgcaggcga gcgcggagat gcgtcggccg agctcgttga
    12481 aacgccatag gcaagcgtcc agacgcttgt catcgccacc ccagcgagca cgaccgccaa
    12541 cggcgagccc catagattcc cgagccgcac gccctcacaa tggcgagccg gctcgatcca
    12601 gtcaaattga cgtgaacgcc ccaccgaagt cggctatgat gcgcgccatg aagtcgcgcg
    12661 agctgctctt gaaggcagca aaattcgacg ttgttcgcct gcggttcgaa ggccctgacg
    12721 gcaaggagat cgtccgcgac ctggtcgaac accccggtgc agcggtcatc ctaccccagt
    12781 tggacgacgg acgtatcgtc ttgatacgca a
//
LOCUS       DASRZF010000293         5993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1941568, whole genome shotgun sequence.
ACCESSION   DASRZF010000293 DASRZF010000000
VERSION     DASRZF010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 5993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5993
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1941568"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="VFG22_12790"
     CDS             complement(<1..605)
                     /locus_tag="VFG22_12790"
                     /inference="COORDINATES: protein motif:HMM:NF015915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HET6417166.1"
                     /translation="MVTKPNSLEQNLPPSVVSAVQRFMKAGFWNRWAEATEAHLQEPA
                     QQFDPDFMRRLVAKMEIFTSYFGAEVRGMDRVPKSPVLLIGNHSGGIITPDTSAVYAA
                     WYRARGFDDPLMGLAFDGIYGVPGWRELMRKIGQMPASMDNAEAALGQGHSVLLYPGG
                     AYEVFRPWKDRNRIAFKGRKGFIRLALRAGVPVVPVVGHGG"
     gene            658..1872
                     /locus_tag="VFG22_12795"
     CDS             658..1872
                     /locus_tag="VFG22_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417167.1"
                     /translation="MVAARVWSGLVHRLFPWRAPVSQKNRRRRRPVPVRAARQETGAR
                     IRLARSNAYTGVARFTGRSIDRMFLAAMSRPHRYPGAGDLARVGEEIRAAHALYRKGR
                     PAAPSAFHAAPPPLEHPSIESAWHPSMRFEWLSFESGYAAAPGDPAAQRWNNYDRNHV
                     AHAWVARHSGPERPWLICLHGLGTGGPYADFAGFRAEMLHHELGLNLLMPVLPLHGPR
                     KPAALPIASLLSFDLLDAFHGVSQAMWDTRRLIQWARAQGATKIGVYGLSMGSYVGAL
                     LSGFENLDVVIAGIPVCDIPDLFVHHAPVDQRQEAEDNGVLGVQTRELFELVSPLSVA
                     PKTTLENRFIFAGSDDKVSTPEQAERLWEHWGQPGIRWFAGGHVSFFWSSEVAPFVDH
                     RLHKAGFAVLDH"
     gene            1881..3281
                     /locus_tag="VFG22_12800"
     CDS             1881..3281
                     /locus_tag="VFG22_12800"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HET6417168.1"
                     /translation="MQRFTGQDASFLYMETPSVHMHTLKVAVIDLPAQIPYADVFDLV
                     RISMESKLHLVPRLRLRPVEVPGGLHHPMWVNDPNFKIGRHLYRSKIASPGGRREMDR
                     AIGEIASIQLPRDRPLWETWLLEGLADGGIVLVTKIHHSLADGVASAAMLAKVMSLEP
                     DPDEVNVPNPAWVPEPIPSRRTLIRDALRDQRVQLRSIGPLVKRTVEGSVSVLKNIKH
                     RAIDVPYPFVTPRTVFNRSLSPHRSFATTSLPLAELKLVKDAAGVTLNDVVLAIVAGA
                     LDRFFEARGETLSKPLIVSVPMAVVNPGDAPREAGNRVANLFTCLCNDVTDPLERLKK
                     IHAVTKQSKEVQRQLGIETLLDWSEAAPALAYHWALAAFSQSGLSDIMPPAANLIVSN
                     VRGPDHPLYVAGARLRTIYSVGPAIEGIGLNITGWSYCGSLDFALIADAEAIPEPHTI
                     TDAFRPALDTLLRATV"
     gene            3286..3693
                     /locus_tag="VFG22_12805"
     CDS             3286..3693
                     /locus_tag="VFG22_12805"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET6417169.1"
                     /translation="MSHPAIDAWHQMIRDRNAGALDEVLADDAVFHSPVVHRAQEGKQ
                     LTKLYLSAAIMVLGNAKFTYVREIMGESDAMLEFTAELNGIQINGVDIIHWNADGKID
                     DFKVMIRPLKAVNLLHALMKQMLGQMGSGAAPS"
     gene            complement(3686..4192)
                     /locus_tag="VFG22_12810"
     CDS             complement(3686..4192)
                     /locus_tag="VFG22_12810"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HET6417170.1"
                     /translation="MIFRGKEKPKSVPPEARDRLAEIVEKYLADADDSTRRIVTAVAG
                     LLAKIAYADGHYSTQEEATIQKELSRVHGLSQAGVDAICGLLADQISHVALLGDHDWT
                     RDLRDLADRELRLEVLEVLVEMAVADEVLKHEEQTQLRRIAKALSLTQDEYNAIQARH
                     RDKLSTLS"
     gene            complement(4234..4449)
                     /locus_tag="VFG22_12815"
     CDS             complement(4234..4449)
                     /locus_tag="VFG22_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417171.1"
                     /translation="MFEPGYLIVSLLVSSVGFVMFMYGKKQNRPVQFGGGLLLMIISF
                     FIYDPLWLGLAGTAICGLVWGGVKAGL"
     gene            complement(4545..4901)
                     /locus_tag="VFG22_12820"
     CDS             complement(4545..4901)
                     /locus_tag="VFG22_12820"
                     /inference="COORDINATES: protein motif:HMM:NF018777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EthD domain-containing protein"
                     /protein_id="HET6417172.1"
                     /translation="MIKLVQCVRRKPGLSKQDFRKHWTAYAGQVKRIADASNAKRHVT
                     STALVVDQNMDIMQSRGTKAPYDGMVEVWWQRGADVVAFLESGAGDDLLEDLQRTQES
                     FMDLPNCTFFFASEDS"
     gene            complement(4984..5868)
                     /locus_tag="VFG22_12825"
     CDS             complement(4984..5868)
                     /locus_tag="VFG22_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417173.1"
                     /translation="MPKGGTPEEELKALLPASIAVRFDEMFPEVRNGKVRRRDRKRAE
                     MLRKAEPVLQRVLNPMEVVRFATNGVRQLTWWLLTAGSMNPFANRTTLVLTDRRVLLI
                     HTDSKQRPRMFANQLPLERIRSTSGRNSYIFIRSGREQLMFHGVKRSEARVLKSFLES
                     TATKEGGWQQLCPHCFAATDDAPPFCAKCGEEFKSPKKAALRSLIFPGLGDFYLGYRK
                     YAILEVLGASLLWALFLSTLIPAVTRRGIEGVLVAGPIFALVAFIHIGDALLTRAKAQ
                     TGLHSKDGKLPTGVEPEA"
BASE COUNT         1070 a         1957 c         1831 g         1135 t
ORIGIN      
        1 tgcccaccgt gcccgaccac cggaaccacg ggaacgccag ccctgagcgc cagccgaatg
       61 aaacccttgc gccccttgaa cgcgatgcgg tttcggtcct tccagggccg gaacacttcg
      121 taggcgccac ccgggtagag aagcaccgag tgtccttggc cgagcgcggc ctccgcgttg
      181 tccatgctcg ccggcatctg accgatcttt cgcatgagct cccgccatcc cggcacgccg
      241 tagatgccgt cgaacgcgag ccccatgagt gggtcatcga acccgcgcgc tctgtaccag
      301 gccgcgtaca ccgcgctcgt atccggcgtg atgatcccac ccgagtggtt gccgatgagc
      361 aggactgggc tcttcggaac gcgatccatc ccgcgcacct cggcaccgaa gtagctggtg
      421 aagatctcca tcttcgcgac gagccggcgc atgaagtccg gatcgaactg ctgcgcgggc
      481 tcttggaggt gcgcttcggt ggcctcggcc catcggttcc agaacccggc cttcatgaag
      541 cgctgcaccg cactcactac actcggcggc aggttctgct cgaggctgtt tggtttggtg
      601 accatcgggt tgcctgaaaa tagtacatgt tctattttag atcgactgga gaatcacatg
      661 gtggctgcgc gcgtctggtc gggtctcgtt catcgcctgt ttccctggcg cgccccggtt
      721 tcccagaaaa accggcgccg gcgcaggccg gttccggtcc gcgccgcgcg gcaggagacg
      781 ggtgcccgga tccggctggc ccgaagcaat gcctacacgg gcgtggcgcg cttcaccggt
      841 cgctcgatcg atcggatgtt cctagcggcc atgagccggc cgcaccggta tccgggtgcc
      901 ggggacttgg cccgggtcgg agaggagatc cgcgccgctc atgcccttta ccggaaaggg
      961 cggccggcgg ctccgagcgc ttttcacgct gcgcctccgc ccctcgagca tccgtcgatc
     1021 gagtcggcct ggcatccttc gatgcgcttc gagtggctct cgttcgagag cggttatgct
     1081 gcggcgccgg gcgatccggc cgcgcaacgc tggaacaact acgatcgcaa tcacgtcgcc
     1141 catgcctggg tggcgcgcca cagcggtccg gagcggccct ggctgatctg cttgcatggg
     1201 ctcggcaccg gcggacctta cgccgacttc gcaggctttc gtgcggagat gctccaccat
     1261 gagctcgggc tcaatctgct gatgccggtt cttccgctgc acgggcctcg gaagcccgcc
     1321 gcgttgccga tcgcctcgct cttgtcgttc gatctgctcg acgcatttca cggtgtctct
     1381 caagcgatgt gggatacgcg tcgcttgatt caatgggcgc gggcgcaggg cgcaaccaag
     1441 atcggcgttt atgggctctc gatgggttcg tacgtggggg cgctgctctc cggattcgag
     1501 aacctcgacg tcgtgatcgc aggcatccct gtctgcgaca tcccggatct cttcgtacac
     1561 catgcaccgg tcgaccagcg ccaagaggcc gaggacaacg gtgttctcgg ggtccagacg
     1621 cgtgagcttt tcgagctggt ctcgccgctc tcggtcgcac ccaagaccac cctcgagaac
     1681 cgattcatct tcgctggaag cgatgacaag gtctcgaccc ctgagcaggc ggagcgcttg
     1741 tgggagcact ggggtcagcc aggcattcgc tggttcgcgg gtggacacgt gagcttcttc
     1801 tggtcttcgg aggttgcgcc cttcgtcgac catcgtcttc acaaggccgg cttcgccgtc
     1861 ctcgatcact gaggcccgaa ttgcagcggt ttactgggca ggacgcctcg tttctctaca
     1921 tggagacccc gagcgtgcac atgcacactc tgaaggtcgc cgtgatcgat ctgccggcgc
     1981 agatccctta cgcggacgtg ttcgacctcg tacggatttc catggagagc aagctccacc
     2041 tggtgccaag gctccgtctc cgaccggttg aagttcctgg cgggctccat catccgatgt
     2101 gggtcaacga cccgaacttc aagatcggtc ggcacctcta tcgctcgaaa atcgcctcgc
     2161 caggcgggcg gcgcgagatg gaccgggcaa tcggggagat cgcgagcatc cagcttccac
     2221 gtgatcgccc gctctgggag acctggttgc tcgaaggtct cgccgatgga ggcatcgtct
     2281 tggtcaccaa gatccatcac agccttgcag acggcgtcgc ctcggccgcg atgctggcca
     2341 aggtgatgag cctcgagccg gacccagacg aggtcaacgt gccgaatccc gcctgggttc
     2401 ccgaaccgat tccctcccgc cgcacgctca tcagggacgc ccttcgcgac cagcgcgtgc
     2461 agctccgctc gatcggcccg ctggtgaagc ggaccgtcga aggcagcgtg agcgtcctca
     2521 agaatatcaa gcaccgagcg atcgacgttc cctatccctt cgtcacgccg cgcaccgtct
     2581 tcaaccgctc gctttccccg catcgctcgt tcgcgaccac ctcgctcccg ctcgccgagc
     2641 tcaagctcgt gaaagacgcc gcgggggtga cgctcaatga cgtggtgctc gcgatcgtcg
     2701 cgggggcgct cgaccgattt ttcgaagcga gaggtgaaac cttgtccaag ccgctgatcg
     2761 tcagcgtgcc catggcggtc gtgaatccgg gcgatgcgcc gcgagaggcc gggaaccgcg
     2821 tggccaacct cttcacctgc ctgtgcaacg acgtgaccga tccgctcgag cgattgaaga
     2881 agatccacgc ggtcacgaag caatccaaag aagtccagcg ccagctcgga atcgaaacct
     2941 tgctcgactg gtccgaggcg gcgccggcgc tggcctatca ctgggccctc gctgcgttct
     3001 cccagtcggg gctctcggac atcatgccac cggcagcgaa cctgatcgtc tcgaacgtgc
     3061 gcggcccgga ccatccgctc tacgtcgcgg gcgcgcggct tcgcaccatt tactccgtcg
     3121 gccccgccat cgaagggatc ggcctcaaca tcaccggctg gagctattgc ggaagcctcg
     3181 actttgcgct catcgcggac gcagaggcga tcccggaacc ccacaccatc accgacgcct
     3241 tccggcccgc cctcgacacg ctcctgcgtg ctacggtctg acaccatgag ccatccagcg
     3301 atcgatgcct ggcatcagat gattcgagat cggaacgcgg gggccttgga cgaggtcctt
     3361 gccgacgacg cggtctttca ttctcccgtc gtgcaccgcg cgcaggaggg caagcagctc
     3421 acgaagctct acctcagcgc tgcgatcatg gtgctcggca acgccaagtt cacctacgtc
     3481 cgcgagatca tgggcgagtc ggacgccatg ctcgaattca cggcggagct caacgggatt
     3541 caaatcaacg gcgtcgacat catccactgg aacgccgatg ggaagatcga cgacttcaag
     3601 gtgatgatcc gcccgctcaa ggcggtgaac ctgctacacg cgctgatgaa gcagatgctc
     3661 ggccagatgg gcagcggagc cgcgcctagc tgagcgtcga tagcttgtcg cgatgcctcg
     3721 cctgaatcgc gttgtactca tcctgcgtga ggctcagcgc cttggcgatg cgccggagct
     3781 gggtttgctc ctcgtgcttg agcacctcgt cggcgaccgc catttccacc aggacctcga
     3841 gcacctcgag gcgtagctcg cgatcggcga ggtcgcgcaa gtcccgcgtc caatcgtggt
     3901 cgccgagcag cgccacgtga ctgatctggt ctgccaagag cccgcagatc gcgtcgaccc
     3961 ctgcctggct gagcccgtgc acgcggctca gctctttttg aatggtagcc tcttcctgag
     4021 tcgagtagtg cccgtcggcg taggcgatct tggccagcaa gccggcgacc gccgtgacga
     4081 tgcgccgtgt cgaatcgtct gcatccgcga gatacttctc gacgatctct gccaggcgat
     4141 cccgcgcttc gggcggcacc gacttgggtt tctccttgcc tcggaaaatc atcgcagctc
     4201 caatagaccg cccgcagacg atccacaagc gcgctagagc ccggccttca cgccgcccca
     4261 aacgagcccg cagatcgcgg tcccagcgag cccgagccaa agtgggtcgt agatgaagaa
     4321 cgagatgatc atgagcagca atcctccgcc gaactggacg gggcggttct gcttcttgcc
     4381 gtacatgaac atcacgaagc cgacgctgct caccagaaga gaaacgatca ggtatccggg
     4441 ttcaaacatg cttacctagg cctgtatgcc acggtggcag aggaggggcc cgctagcggg
     4501 atcatcttcc ttctgccgcg tttgcgaacg cagctgagaa aacctcagga gtcctcgctt
     4561 gcgaagaaga acgtgcagtt gggcaaatcc atgaacgatt cctgcgtgcg ctgcaggtct
     4621 tcgagcaaat catcgcccgc gcccgattcc aaaaacgcga ccacgtcggc accgcgctgc
     4681 caccaaacct cgaccatgcc gtcgtacgga gccttcgtgc cacgcgattg catgatgtcc
     4741 atgttctggt cgacgacgag cgccgtgctc gtcacgtgtc gcttggcgtt tgatgcgtcc
     4801 gcgatgcgct tcacttgacc ggcgtacgcc gtccaatgct tgcggaaatc ttgcttcgaa
     4861 agaccgggct tccgccggac gcactgcacg agcttgatca tgaaacctcc gaagtcacga
     4921 gctgaagcat agctcagctc cccaagcccc cgttcgtcaa aaaatcaggc ctcgggtagg
     4981 acttcatgcc tcaggctcga cgcccgtggg gagcttgccg tccttcgagt gcaggcccgt
     5041 ttgtgccttt gcgcgggtca acagagcatc gccaatgtgg atgaacgcca cgagcgcgaa
     5101 gatggggccg gcgaccagca cgccttcgat gccgcgcctc gtgacggcgg ggatcagggt
     5161 gctgagaaag agcgcccaaa gcagcgaggc tcctaagact tccaagatcg cgtacttgcg
     5221 gtagccgagg taaaaatcac ccaggcccgg aaagatcaag gagcgaaggg cggctttctt
     5281 tgggctcttg aattcctcgc cgcacttcgc gcaaaagggc ggcgcatcgt cggtggcggc
     5341 gaaacaatga ggacagagtt gctgccagcc gccttccttc gtagccgtcg attcgagaaa
     5401 gctcttgagc acccgcgcct cggacctctt gacgccgtga aacatcaact gctcacgccc
     5461 ggagcggatg aagatgtagc tgttgcggcc cgaggtcgag cgaatgcgct cgagcgggag
     5521 ctgattggcg aacatgcgcg gacgctgctt cgaatcggtg tggatcaaga gcacgcgtcg
     5581 atcggtgagc acgagcgtgg tgcgattggc aaaaggattc atcgaaccgg cggtgagcag
     5641 ccaccaggtg agttgacgaa cgccattggt ggcgaagcgc acgacctcca tcggattgag
     5701 cacgcgctgc agaacgggct ccgcttttcg cagcatttcg gcgcgcttgc gatcccggcg
     5761 gcggaccttt ccattgcgga cctccgggaa catttcgtcg aagcggaccg cgattgacgc
     5821 tggcaaaagc gctttgagct cctcttctgg tgtacccccc ttaggcatcg gcagcgcgta
     5881 gcttagcatg cccgagacgc tctcattcgg cgctgctcag cgcgtcagga tggtgctagt
     5941 atcgcccctc gttcgtgttc ttgggggagc tctcgatgat ggatccattc ttg
//
LOCUS       DASRZF010000294         2560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4687994, whole genome shotgun sequence.
ACCESSION   DASRZF010000294 DASRZF010000000
VERSION     DASRZF010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4687994"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="VFG22_12830"
     CDS             <1..617
                     /locus_tag="VFG22_12830"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HET6417174.1"
                     /translation="AIRTREHTKSGLVAVTTAFKHAYRALGLQMAKEGLLADQDAVFF
                     LTHQELGELLRGDRELAGRALARRKVIDYQMPLHFPEIFRNRPEPLPLEATDGGEGAL
                     IGKPVSRGSVTGRARVVETLEEAAALQAGEILIAPITDVGWTPYFSLIAGLATDVGSA
                     VSHGAVVAREYGLPAVVNLRVATSRFRTGDLVTLDGDRGTLTRA"
     gene            690..1844
                     /locus_tag="VFG22_12835"
     CDS             690..1844
                     /locus_tag="VFG22_12835"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HET6417175.1"
                     /translation="MVATIGLTGLRPDPARVSKIAAPPYDVIKPGTPLEKLLKNTASS
                     LYHISLGDQPAAARDRMVADGSLIEDDEPAYYVYEQTWPEGQRTGVFVAAEVTPYSAK
                     QVIRHEKTFDDKVKGRMTMRRATGLHLGPVFVLTRAPIAATLERAKGEAELVYDFVSE
                     FGGFSELEGIRNKVWRVPEASEIGRLLKAALATEPFYIADGHHRYHASLLNEQTHFLC
                     YVTEEAVIQAYNRVINGVRPFSEIAGELPLRPVDSFRTPAKHSFCIYTKQGCFELDAQ
                     HVPKDVVGQLDCAILERELYPKLGLTHDMIVDPNHFDYYSESALGEMKAVVDRGEYDI
                     AVALHPVSLEDLMAVADAGLDNPDIVMPEKSTFFQPKILSGLSLYRFKKK"
     gene            1841..>2560
                     /locus_tag="VFG22_12840"
     CDS             1841..>2560
                     /locus_tag="VFG22_12840"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HET6417176.1"
                     /translation="MSSLPQYGTHLHPHEKMRAVVIDEYGGPEVLRATTVPRPAPRRG
                     QVLVRTRFIGVNPKDVIVRKGKFQIATGKKFPLIVGHDIAGEVIEAGVGSDLYEGELV
                     FGMINDFAGRAYAEYAAVDAQQLAKVPSSIELRVAAAVPLAAQTALQALRDDAHLKPG
                     QNVLINGASGGVGVFAVQIAKILGAHVTAVCSHRNTELVRELGADRVIDYTKTDLVDL
                     QERFDAIFDVFGNYSFDRLKHL"
BASE COUNT          522 a          787 c          776 g          475 t
ORIGIN      
        1 cagcgattcg gactcgcgaa cacaccaagt cggggctggt ggcggtcacg acggcgttca
       61 aacacgcata tcgagcgctc ggattgcaga tggccaagga aggcctcctc gctgatcaag
      121 atgcggtctt ctttctgacc caccaggagc tcggagaatt gctgcgcggt gaccgtgagc
      181 tagccgggcg agcgctggcg agacggaagg tcatcgacta ccagatgccg cttcacttcc
      241 cggaaatctt tcgcaacagg cccgagccgc ttccgctgga ggcgaccgac gggggcgagg
      301 gagcgctcat cggcaaaccg gtgagtcgcg ggtcggtgac agggcgtgcg agggtcgtag
      361 aaacgctcga ggaagcggcc gccttgcagg cgggcgagat ccttatcgcg ccgatcacag
      421 acgtcggatg gacgccctat ttcagtctga tcgcggggct cgcaaccgac gtcgggagcg
      481 cggtttccca tggcgccgtg gtcgctcggg aatatgggct tccagcggtc gtgaacctca
      541 gggtcgcgac ctcgcgcttt cgaacgggcg acttggtcac cctcgatggc gaccgcggaa
      601 ccctgacgcg cgcctagggc cgggggcgcc tcggggtgct cttgacttcc aggggcaata
      661 tgcgatccgc aggcccctaa ggagctacca tggttgccac gattggactt actggattgc
      721 gcccggaccc agcccgcgtt tctaagatcg cggctccgcc gtatgacgtg atcaagcccg
      781 gcactcctct tgagaagctg ctcaagaaca ccgcaagctc gctctaccac atcagcttgg
      841 gggatcagcc agccgctgcc agagaccgca tggtcgcgga cgggtcactc atcgaagacg
      901 acgaacccgc ttactacgtg tacgagcaga cctggccaga aggacagcga accggcgtgt
      961 tcgtcgctgc ggaagtcacg ccgtattctg caaagcaagt cattcggcac gagaagactt
     1021 tcgacgacaa ggtcaaaggg cgcatgacga tgcgtcgcgc aaccggcctt cacctcggac
     1081 cggtcttcgt gctgacccgg gcaccgatcg cagcgacctt ggaaagagcg aagggggagg
     1141 ccgagctggt ctacgatttc gtcagcgagt tcggtggttt cagcgagctc gaagggatcc
     1201 gaaacaaggt ttggcgcgta cccgaggcga gtgagatcgg gcgtttactg aaggctgcgc
     1261 tcgccaccga gcccttctac atcgcggacg gacaccaccg ctaccacgca agcctactca
     1321 acgagcaaac gcatttcctc tgttacgtca ccgaggaagc ggtcatccag gcttacaacc
     1381 gagtgatcaa cggagttcgc cctttctcgg agatcgccgg tgagctgccg ctgaggcccg
     1441 ttgattcgtt ccgcaccccc gcaaagcaca gcttttgcat ctataccaaa caggggtgct
     1501 tcgagctgga cgcacaacac gttccgaaag acgtcgtggg ccaactcgac tgcgccatcc
     1561 tcgaacgcga gctctacccg aagctcggcc tcacacatga catgatcgtc gacccgaatc
     1621 acttcgacta ctattcggaa tctgcgctcg gcgagatgaa ggccgtggta gaccgaggtg
     1681 aatacgacat cgcggtagcc ctccacccgg tgagcctcga ggacctgatg gcggtggccg
     1741 atgctggtct cgataatccc gatatcgtca tgcccgaaaa gagcaccttc tttcagccga
     1801 aaattctgtc cggcctgtcg ctctaccggt tcaagaagaa atgagctcgc ttccgcagta
     1861 cggaacgcac ctgcacccgc acgagaagat gcgcgccgtg gtgatcgacg agtacggcgg
     1921 ccccgaggtg cttcgagcga cgaccgtccc gcgtcccgcg cctaggcgcg gacaggttct
     1981 ggttcgaacc cgcttcatcg gcgtaaatcc gaaggacgtc atcgtccgca agggaaagtt
     2041 ccagattgca accggtaaga agtttccgct catcgtggga cacgacatcg ctggtgaggt
     2101 catcgaagcc ggtgtcggct cagatctcta cgaaggcgaa ttggtctttg gaatgatcaa
     2161 cgactttgca ggccgggcct atgccgagta cgcggccgtc gacgcccagc aactcgccaa
     2221 ggtgccttcc tcgatcgagc tgcgcgtcgc tgcggctgtt ccgctcgcag ctcaaacagc
     2281 cctgcaagcg ctgcgcgacg atgctcacct gaagcccggt cagaacgtct tgatcaacgg
     2341 cgcatcgggt ggcgtcggcg tctttgcggt ccagatcgcg aagatcctgg gtgctcacgt
     2401 caccgccgtc tgcagtcacc gaaacacgga gctcgttcgt gagctcggcg cagatcgtgt
     2461 catcgattac accaaaaccg atctggtcga cttgcaagaa cgcttcgatg cgattttcga
     2521 tgtctttggc aactactcat tcgacaggct caagcatttg
//
LOCUS       DASRZF010000295        16709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3659239, whole genome shotgun sequence.
ACCESSION   DASRZF010000295 DASRZF010000000
VERSION     DASRZF010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 16709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16709
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3659239"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="VFG22_12845"
     CDS             complement(<1..929)
                     /locus_tag="VFG22_12845"
                     /inference="COORDINATES: protein motif:HMM:NF024778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HET6417177.1"
                     /translation="MDGQPIEHHSSGMRTRIWAGLLMFVATFAPQTHASADTLSELTA
                     PFADALHGGSYSGALGLVFLAGLATSLTPCVYPMIAITVSVFGARQAETRARAALLST
                     AFVLGIAALFTPLGVISALTGSAFGSALANPWVLGALAGLFTLMALSMFGLFDLALPS
                     SLQNRLAEAGGFGLRGAFVLGFVNGLIAAPCTGPVLAVLLTWVATTGNLAFGALALFV
                     YSIGIGVLFWFVGTFAITLPKSGRWVEWTKSAFGIAMLALAFYYLRGVLPYPRPAVRD
                     PMWLAVAFGLLAGGVITGAIHLSFASGAWGARFR"
     gene            982..1518
                     /locus_tag="VFG22_12850"
     CDS             982..1518
                     /locus_tag="VFG22_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417178.1"
                     /translation="MLISIATGALSAHAGRDEIRNSADPVWRMEGFLAYALFVALVLV
                     PTATYFYVFHGDWFLFYWIETDRAPWFWGLVAVSLVLGAASLGFLLGAALCRASRDRL
                     VRRFSAGVILMALAIWPSIWHRLSVVGSSRQFARDYGLTAFFSSPAFYSGLVTGLLLL
                     AAFVWLVFRIDRQTRDRS"
     gene            complement(1534..6603)
                     /locus_tag="VFG22_12855"
     CDS             complement(1534..6603)
                     /locus_tag="VFG22_12855"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6417179.1"
                     /translation="MGFDNGEITALRRDATEELGQDDLVQEPDALGPPTLPPPPRPEV
                     QRAPSPSRADRTRRARDWVEECEAELRGQPDARRAARLYFEMARACEGPLKDARRAVQ
                     YYQAALEHAPDYVPAIRAARRLLLGARMFADALALFDAEARVTRQPRDKATLYLLKGR
                     LLEDVLGDRDAAEEAYHTARALDRSHAAILRATEQRSFAKQTWSEVDRTLAELANASA
                     SSDEHRAALIIRRAQLLESRQQRPEQAAELYETALQLDPSALIAIDALKRLYYGQRRW
                     RDLIRTLEAEANLVGDPGLKASSLYRAARIHAELLGNRDEAIRVLEQAQALEPTDPLI
                     LGELARHYEAAQRLQALVETLEALWHASDDEAERLTYAQRVARLYEEDLRDVDQAIAW
                     HERAIELSPTSLSSLQALSLLYRVWGAWPKLIEMCLQEARYTTDAQRRADSHTRAAEI
                     YETKLNAIDDAIEHYHRALNAMPDHPTAFKALSRLLADVQQPKELIQLYENALDRATG
                     TRAVSYLLKIAQVYEDVLGEHALAAQTYRRVLQQDSSHLTAVHAWQRSAARAQRPREL
                     LEALDYEIDLTVERERLVALLHRSGEVLDEQLGDRDEAITRYRRALEKEPRHVPTLAS
                     LGRIYYAAGRWNDLLDVYGQELRLTSGKQRRVALLQKMAEISRDRLGSDEDALRYHRE
                     AVKVDPGHAPSLQELARHLRERGDWEQLVDVLELQLRNSSAAQNRARAAFVLGQVYEE
                     HLGDQAKAVASYEAAASADASYRPAVDALARVRAEAQDWPHMVADLERELKLTQAPAQ
                     RVALFARKAQVLEWQDKHRQAIQAWEDVLEIEPKHLGALLALEQLHRQVGAWESLCGV
                     YSTQAEVMQDSSGRVAALYELARLLEHQGLGEYDAIVQAYRRILEIAPADLGALIELE
                     RLAAGRRDADLLREVDILAAKSTHDPTVAAAHRARLAELVEGDEDGQAAEQFRAAAMA
                     DPYHIGAARGLTRVAQETDDPAALVDALVREAEAERDAKAASDLYVRSATVRLQRIGD
                     HVGARRDLERALELWPDSAEAASGLIEVLTIDLDWSSLVDRLSQAASAAGSVERASAL
                     LLAVARIQREELDELPAAIRTLQRVLKVQPRHLDALERLAEHFRANGSWHEAAATLEQ
                     LLAAAPENSRLRRALLSLAQIYRERLDMPEKALRHVEAALAAEPTHPAALHELSLAHE
                     ALGQFPEALQVARHLLDGRDGKERADALIRLGQLEHATGNAGEARIALRRAVVLEGPG
                     SDAARALEQLCETPEDWRDYADALGQHGAKDEAGRVGTALEVARILSDHCRDERAAIA
                     ALRGALEQGAADADIRRELAVRLRARGEQTAAIEQLQTVLSIEPLREEIWRELAISYE
                     ADGRAREARIATLPLLALACDDEEDRVRAASVESWPARVRPHSLRGSVLERLGTPRAE
                     DAAAGALLAALSPALAKLYPPDLESYGLATRDRLPTEVNHPLREIANSISAALDIRSF
                     DLFLHRVRNRGITIEFGVHPAVLVPATIMEKDACTQTFMLAQPMTQIARGYQAVDKLT
                     PRELDVLLASAARTVRPDFGVGLTSEEFLNEQTRRLQRAIPRRDRKLVRDAALAYSQA
                     KRVKFDRWVNAAQRTAIRVALLLCDDLSPLVQDVRSRIAPEREAEGETFETHPTFIDA
                     MKFWASPAAMHLREHMGMLPR"
     gene            complement(6688..7524)
                     /locus_tag="VFG22_12860"
     CDS             complement(6688..7524)
                     /locus_tag="VFG22_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6417180.1"
                     /translation="MQSSSSLDRYIQQVRAIPKLSREQEHELALRALEGDQKASDRLV
                     EANLRYVIAIALQYRRYGIKLGDLIAEGSVGLVTAVRKFDPHRGTRFVTYAGYWIRAF
                     VLEAVVRSSTMVGAGSGPFRSKLFFRLRRERARLSNLIADPDELIEALASEFETSKEK
                     MTDLLRRLDQREISLDAPAYQDSDSTLVELLPGTYEPQDQVVARQRRQSGIQMRLEGA
                     MSLLDERERLIVEKRILSDDAASLAALGRELGVSRERARQLEARAKKKLAEELRDLAP
                     AA"
     gene            complement(7704..8027)
                     /gene="erpA"
                     /locus_tag="VFG22_12865"
     CDS             complement(7704..8027)
                     /gene="erpA"
                     /locus_tag="VFG22_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HET6417181.1"
                     /translation="MLNLTPRAADKVKEIREAEGLAEQGLRVRVIGGGCAGFSYDLFF
                     EDETSELDQTFTSHGIPIYVDMMSAQYIEGTEIDYVEGLQGAGFKFVNPQASQTCGCG
                     SSFAT"
     gene            complement(8095..9021)
                     /locus_tag="VFG22_12870"
     CDS             complement(8095..9021)
                     /locus_tag="VFG22_12870"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417182.1"
                     /translation="MDQARELEWLTQSAGIVALDDRAIVSVGGDDAHDWIQGQVTNQV
                     EGVPAGGSVYAFVLTLKGRTLADVYVLMGDYEVLLDVPKAQVDSLLARLERYIIMEDV
                     DLEHRPDLGLLAAVGPLAHEVGEGGWPADRLGVGGRQWVVPTDALSSERDRLIARCHA
                     LGGGLVGEKTWRYAHVLLGRPLFGIDFGDWTYPQETGLNELAVSFKKGCYIGQETVVM
                     LQNRGKAPKVLWRWAIEGRDPPEAATPILHGETVVGEITSAVTEGDRSLALGFLKRGH
                     EGQGPEGFSVAGRPARALGPVAGGPGLGVSSA"
     gene            complement(9066..9848)
                     /locus_tag="VFG22_12875"
     CDS             complement(9066..9848)
                     /locus_tag="VFG22_12875"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HET6417183.1"
                     /translation="MIEDLRTERLQERISGTWGRSLEVLESTASTMDVAAAAAANGAA
                     DGHLVLADQQTQGRGAHGRHWVSPPGSDLYFSVVARPAVEPASTALVTLASGLGVRDA
                     VAGLLPERSVRVKWPNDIWIGRRKCAGVLVESRTLGMRIDSVIIGVGLNVNRLQWPPD
                     LAGTATSLRAERGVEEPFDRAEVLAAVLSNMERWVKRFIQDGAPVVVNALRSRLALLG
                     ERVRWEDGVGSFDGIDEDGAARVRTETGVLSLHSAHLEPITD"
     gene            complement(9852..10700)
                     /gene="nadC"
                     /locus_tag="VFG22_12880"
     CDS             complement(9852..10700)
                     /gene="nadC"
                     /locus_tag="VFG22_12880"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HET6417184.1"
                     /translation="MQVTPPAPTILAETVRRALSEDIDRGDVTTEACVDPNQIGAATL
                     RAREPLVVCGLPVAEEVYAQLDLLIDVDTLVEDGDRVEAGSPLAQISGPAASILLGER
                     VALNFMQRLSGVASMTRRFVDALPEGSPTRITDTRKTTPGLRAFERYAVRCGGGHNHR
                     EDLGAAVLIKDNHIAAAGGCKEAIERARAYAPHTCRIECEVDTPAQLQEALAARADII
                     MLDNFDDDAVRDALRSIAGRAIVEVSGGITLERVKSLGALGVDVISVGALTHSAPSCD
                     LGLDWS"
     gene            complement(10708..11448)
                     /gene="mrtC"
                     /locus_tag="VFG22_12885"
     CDS             complement(10708..11448)
                     /gene="mrtC"
                     /locus_tag="VFG22_12885"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF040594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MrtC family glutamic-type intramembrane
                     protease"
                     /protein_id="HET6417185.1"
                     /translation="MAEAHAPLRQIFWVYALVCASVFAVTRLESSGALGRYVHLLVAA
                     IFLLTAIRFTRAEPKHYGVSLGGLLEPPTDDRPEGPLGLFDLGRAIVDALPSAAVELG
                     FAFAVAAIVFPIYALGFYAWNQPAQPFSLALPPSLPSFVLAQLIVVALPEEAFFRGYL
                     QTALSDLRAKRLRVLGVALEPGAWLLQAVLFAAIHFMVEPHPARLAVFFPALLFGWTR
                     AWRGGIGAALALHAMSNLYSEILARSWL"
     gene            11549..13381
                     /gene="dnaG"
                     /locus_tag="VFG22_12890"
     CDS             11549..13381
                     /gene="dnaG"
                     /locus_tag="VFG22_12890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HET6417186.1"
                     /translation="MISEDKIAEIRDRAPIADVIGNYVALKKSGNSLKGLCPFHNEKS
                     PSFYVHPSRGFYHCFGCKASGDIFSFLMHVEGKTFPEVARDLAEQTGVELPVYDPQRE
                     AEFQRNKQTSDRLASLIEKATQFYVAQLAEHPDSAIARNEFLSRGISEESAREFRLGY
                     APHGWDSLVRFLEQSGASPADAEAAGLIVSRRSGRGHYDRFRHRLMFPIADVHGKVVA
                     FSGRALTPAPSMEEQKEPPAKYLNSPENPLYHKGEVLYGLHEGRVEIRREAATVLCEG
                     NFDLVALHQGGFRNAVAPMGTAFTEAHARLLKRFAQKVILLFDGDKAGRKAVREAYAV
                     VGKVGLEAHVVTLPQGEDPDSFLRQQGSEALRARIVSAPTIIEYLIDDAAATAAPNPP
                     AKADAIAKLGPILAKVESPIERGLYVERVARKFGIADTVLVRRELRRGLGRGGTRSVP
                     SRDAVQQPIATPVSELQSKLISVLIDQPSLLHEASVQKLPKLLTSPDLRAIFDLILRM
                     VDMQGSLDVTALLDELHDNQLRPWLEAKLAVQEHSLEDARQILDDGLPLLERKNIEHE
                     LPRLQQRIVEARRVGDDALAAGLTREFVELTRSAHRLKSNTKQR"
     gene            13384..15234
                     /gene="rpoD"
                     /locus_tag="VFG22_12895"
     CDS             13384..15234
                     /gene="rpoD"
                     /locus_tag="VFG22_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HET6417187.1"
                     /translation="MASKSSVRDEESNESKFKSEPVSRVVPKVKGRERKEVQVLLERG
                     RQQGHLTYDELNEALPPDMVTSDQIDDLMVWLKSEDIDVVDGAPEGQSRTSEVPVAAT
                     RTTTRRPSAIPPPAEDSYAQKSNDPVRMYLRKMGSVSLLTREGEVEIAKRIEDGEDKI
                     FEVILNSRVGVAEILEIGESLRRGKLRIKDVVRDLDKEEEVDFEAAKVRALKVFDKVK
                     RIESSLAKVRNELDGKRKVSDRSKKHYTAQLDKHIHERIALLRELNLTKKQIDRVVSK
                     IKAYTRRVERAEREMNEAEARVGGLKYEDMKRVVRDMQESKAVEKRVQRKYGSEEQIL
                     MSEEHIRDAQKAIDHVEEDMGQPVERLRNTYRALAEGERQSERAKAELVEANLRLVVS
                     IAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRAIADQA
                     RTIRIPVHMIETINKLIRTSRYLVQELGREPDPEEIAERMEMPLEKVRKVLKIAKEPI
                     SLETPIGEEEDSHLGDFIEDKNAVSPVEAVIGGNLEDQTRRVLKTLTPREEKVLRMRF
                     GIGEKSDHTLEEVGQDFEVTRERIRQIEAKALRKLRHPSRSKQLRSFMDT"
     gene            15676..15749
                     /locus_tag="VFG22_12900"
     tRNA            15676..15749
                     /locus_tag="VFG22_12900"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15710..15712,aa:Ile,seq:cat)
     gene            15829..16542
                     /locus_tag="VFG22_12905"
     CDS             15829..16542
                     /locus_tag="VFG22_12905"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HET6417188.1"
                     /translation="MREELVALAKLAEMDDSARDIDAELKQIPQRLEELRHSVQMLET
                     MLSQERQQLTEAEGLRAQQSAELKERGEGLQRARKKIAQSSSIREADAAEREVEANRR
                     AIKEREDEIGRIAQAIEAKKASLAEREKDFEDTKSSLQADEETSKARIAELEEQRSKL
                     LVGRDELAAKVSKTIVKRYERLKTGIPNAVIIITDGNCPACRMALPAQLTIDLRRADE
                     LHQCPHCRRIIIHKSVLED"
BASE COUNT         3126 a         5345 c         5299 g         2939 t
ORIGIN      
        1 cgaaacctcg cgccccatgc gccgctggcg aaggaaagat gaattgcccc ggtgatgacg
       61 ccgcccgcga gaagcccgaa cgccaccgcc agccacatcg ggtcgcgcac ggcgggacgc
      121 gggtagggca gcacgccgcg caggtagtag aacgccaggg cgagcatcgc gatcccgaat
      181 gcgctcttgg tccattcgac ccagcgtccc gacttcggca aggtgatggc gaacgtgccg
      241 acgaaccaga acagcacgcc gatgccgatc gagtagacga acagggcgag ggcgccgaag
      301 gccaggtttc cggtcgtggc cacccaggtc agcaggaccg caagaacggg accggtacag
      361 ggcgctgcga tgagaccatt gacgaagccg agcacgaatg cgccgcgcag accaaagcca
      421 cccgcctcgg cgagccggtt ctggaggctc gacggaagag cgaggtcgaa gagcccaaac
      481 atgctcaggg ccatcaatgt gaacaggccc gccagcgccc cgaggaccca ggggttcgcg
      541 agcgcgctgc caaatgcgct tccggtcagc gcggagatca cgccgagggg agtgaagagg
      601 gccgcgatgc ctagcacgaa cgccgtagag agaagcgccg cccgtgcacg cgtctcggcc
      661 tgtcgcgcgc cgaagacgct gaccgtgatc gcgatcatgg gatacacgca cggcgtcaat
      721 gaagtcgcca ggcctgcgag aaacaccagt cccagcgccc ccgagtaact tccgccatgc
      781 agggcatcgg cgaagggcgc cgtgagctcg ctcaaggtgt cggcggatgc gtgcgtctgc
      841 ggcgcgaaag tcgcgacaaa catcagcaat ccggcccaga tccgcgtgcg catccctgaa
      901 ctatggtgct cgattggttg accgtccacg aagcggggac gtagcctttc gctggggaac
      961 atcttgccgc tacttttcct gatgctcatc tcgatcgcca ccggggccct ttcggcccat
     1021 gccgggcgcg atgagatccg caatagcgcg gacccggtct ggcgcatgga gggattcctg
     1081 gcctacgcct tgttcgtggc cctcgtcctg gttccaacgg ccacctactt ctatgtattt
     1141 catggcgatt ggtttctgtt ctactggatc gagaccgacc gggcgccttg gttctggggg
     1201 ctcgtcgcgg tttcgctcgt gctcggcgcg gcttctctcg gatttctgct gggcgcggca
     1261 ctctgccgag cgtcgcgcga ccgcttggtt cgccggttca gtgcgggcgt gatcctgatg
     1321 gctttggcga tctggccgtc gatctggcac cggctttcgg tcgtcggttc gtcccgtcaa
     1381 ttcgcacgtg actacggcct caccgcgttc ttctcgagtc cagcattcta ttcgggtctc
     1441 gtcacggggc tgttgctcct cgctgcgttc gtctggctcg tcttccggat cgaccggcaa
     1501 acgcgcgacc gcagttgagg tcccgtcccg cgctcagcgt gggagcattc ccatgtgctc
     1561 gcgcaggtgc atggccgccg gcgaggccca gaacttcatg gcgtcgatga aggtcgggtg
     1621 cgtctcgaag gtctcgcctt ctgcttcgcg ctcaggagcg atccggcttc gcacatcttg
     1681 cacgagcggg ctgaggtcat cgcagaggag cagtgccaca cgaatggccg tgcgctgggc
     1741 tgcgttgacc cagcggtcga acttcacgcg cttcgcctgc gagtaggcga gagccgcatc
     1801 ccgaacgagc ttgcggtcgc gtcgcgggat cgcccgctgc aaacgtcggg tctgctcgtt
     1861 gaggaattcc tcgctcgtca gccccacgcc gaagtccggt ctcacggttc gcgctgctga
     1921 cgccaggagc acatcgagct cccgcggggt cagcttgtcg accgcctgat atccgcgggc
     1981 gatctgcgtc atcggctgtg cgagcatgaa ggtctgcgtg caggcgtcct tttccatgat
     2041 ggtcgcggga accaggaccg cagggtgaac cccaaactcg atcgtaattc cgcggttgcg
     2101 gactcggtgc aggaagagat cgaagctccg gatgtccagc gcagccgaaa tgctgttcgc
     2161 tatctcgcgg agtgggtggt tcacctccgt cgggagccgg tcgcgtgtgg cgagcccgta
     2221 gctctcgaga tcgggtgggt agagctttgc gagcgcgggg ctcagcgcag ccaagagcgc
     2281 accggcggcg gcgtcctccg cgcgcggcgt tccgagtcgt tcgagaaccg atccccgaag
     2341 cgaatggggc cgcacccgcg cgggccaaga ctcgactgac gccgctcgca ctcgatcctc
     2401 ttcgtcgtcg catgccaagg cgagcagcgg cagcgtcgcg atccgcgctt ctcgcgctcg
     2461 cccgtcggct tcgtagctga tcgcgagctc acgccaaatc tcctctcgga gcggctcgat
     2521 cgacagcacg gtctgaagct gctcgatcgc tgcggtttgc tcgcctcggg ctcgcagccg
     2581 gaccgcgagc tcacgccgga tgtcggcatc cgctgcgccc tgttctagag ctccacggag
     2641 agccgcgatg gcagcacgtt cgtcgcggca gtggtcggac aaaatccggg caacctcgag
     2701 cgcagtaccg acccggcccg cttcatcttt tgcgccatgc tgtccgaggg cgtccgcata
     2761 gtctcgccag tcctccggcg tctcacagag ctgttccaat gcgcgcgccg catcactccc
     2821 gggcccctcg agcacgactg cgcgtcgcag cgctatcctg gcctcgccgg cgttccccgt
     2881 tgcatgttcg agctgcccaa gccgaatcag agcatccgcg cgctccttgc catcgcggcc
     2941 atcgaggaga tgtcgggcga cctgcaatgc ctccgggaac tgcccgagcg cttcgtgcgc
     3001 caaagaaagc tcgtggagcg cggcagggtg tgtcggctcg gcagccaagg ccgcttcgac
     3061 gtgtcgaagc gccttctcgg gcatgtcgag ccgctcccga taaatctggg cgaggctcag
     3121 aagtgctctc ctgagacggc tgttctcggg cgctgccgcg agtagctgtt ccagcgtggc
     3181 ggccgcctcg tgccatgacc cgttggctcg aaagtgctcg gcgagccgct ccaacgcatc
     3241 gaggtggcgg ggctggactt tgagcacacg ctgtagggtg cggatcgcgg ccggaagctc
     3301 gtcgagctct tcgcgctgga ttcgcgccac cgccagcaag agagcgctcg cgcgctccac
     3361 cgacccagcc gccgaggcag cttgtgacag ccgatcgacg agtgacgacc aatccaagtc
     3421 gatcgtgagc acctcgatca agccgctcgc cgcttcggcc gaatccggcc agagctcgag
     3481 cgcccgctcc aaatcgcgtc gtgcaccaac atggtctccg atgcgctgta ggcgaaccgt
     3541 tgcgctccgg acatagagat cggacgcggc cttggcgtcg cgctccgcct ctgcctcgcg
     3601 aacgagtgcg tccacgagag cggccggatc gtccgtctct tgcgcgactc gtgtgagccc
     3661 gcgtgcggcc ccgatgtggt acgggtcggc catcgccgcc gcgcgaaact gctcggccgc
     3721 ttgaccgtcc tcgtcgcctt ccacgagctc ggccaatcgc gccctatgcg ctgcggcgac
     3781 ggttgggtcg tgcgtcgact tcgcagccag aatgtccacc tcgcgcaaga gatcggcgtc
     3841 tcgtcgaccc gctgccaatc gttcgagctc gatcaatgca cctagatcgg caggagcgat
     3901 ctccaggatg cgccggtacg cctgaacgat tgcgtcgtac tcgccgagcc cctgatgctc
     3961 caatagcctt gcgagctcgt agagcgcagc gacgcggccg ctgctgtcct gcatgacctc
     4021 tgcttgggtc gagtacacgc cgcataggga ctcccacgct cccacctgcc ggtgcaattg
     4081 ctcgagcgcg agcaatgcgc cgaggtgctt tggctcgatt tccagcacgt cttcccatgc
     4141 ttgaatcgcc tgccgatgct tgtcctgcca ctcgagaacc tgggccttgc gcgcgaagag
     4201 tgcgacgcgc tgcgcgggcg cctgggtcag tttcagctcg cgctcgaggt cggctaccat
     4261 gtgcggccag tcctgcgctt cagctcgcac tctcgccaga gcgtccaccg cgggtctgta
     4321 gctcgcatcc gcgctcgccg cggcctcgta cgacgccact gctttcgctt ggtccccgag
     4381 atgctcctcg tacacttgcc caagcacgaa cgcggcgcga gcacggttct gggcggcgct
     4441 agagttgcgg agctggagct cgagcacgtc cacgagctgc tcccaatctc cgcgttcgcg
     4501 caaatggcgt gcaagctctt gtagcgacgg cgcatgtccg gggtcgacct tgaccgcttc
     4561 acggtgatag cgaagcgcgt cctcgtcgct gcctaagcgg tcgcgtgaga tctcggccat
     4621 cttttgtagg agcgccaccc gccgttgttt cccagacgtg aggcgcaact cctgaccgta
     4681 cacgtcgagc aagtcgttcc aacgaccagc cgcgtagtag atccgtccga ggctcgcgag
     4741 cgtcggcacg tgccgaggtt ccttctcgag agctcgacgg tagcgtgtga tcgcttcgtc
     4801 tctgtccccg agctgctcgt cgaggacttc gccggatcga tgcaagagcg caaccagccg
     4861 ttcccgctcg acggtgaggt cgatctcgta gtcgagggcc tcgaggagct ctcgaggccg
     4921 ctgagcgcga gccgccgagc gttgccaggc gtgcactgcc gtcaagtggc tgctgtcctg
     4981 ctgcaagacg cgccgataag tctgcgccgc cagcgcgtgc tctccgagca cgtcttcata
     5041 gacctgggcg atcttcagca gataagaaac cgctcgcgtc ccggtggctc ggtccaatgc
     5101 attttcgtag agctggatca gctctttcgg ctgctgcacg tccgcgagca gtcgcgagag
     5161 agccttgaac gccgtgggat gatcgggcat cgcattcagc gcgcggtgat agtgctcgat
     5221 ggcgtcgtcg atggcgttga gcttcgtctc gtagatctcg gccgcgcgcg tgtgcgaatc
     5281 ggcgcgcctt tgagcgtccg tcgtatagcg ggcctcctga agacacatct cgatgagctt
     5341 cggccaggcg ccccataccc tgtaaagcaa gctcagagcc tgcaagctag agagcgatgt
     5401 ggggctgagc tcgatcgctc gctcgtgcca ggcgatcgct tggtccacgt cccgtagatc
     5461 ttcttcatac aagcgcgcga cgcgctgcgc gtaggtcagc ctttcagcct cgtcgtcgct
     5521 tgcgtgccag agcgcttcca gagtttcgac cagggcctga agtcgctgcg cggcttcgta
     5581 gtgccgtgcg agctcgccca gaatgagggg gtccgtcggt tccagggcct gcgcttgctc
     5641 gagcacgcga atcgcttcgt cacggttgcc aagcagctcg gcatgaatgc gggcggctcg
     5701 gtaaagcgaa gacgccttga gcccaggatc gcccaccagg ttcgcctccg cctcgagcgt
     5761 gcgaatcagg tctcgccagc gacgctgccc gtagtagagg cgtttcaatg cgtcgatcgc
     5821 gatgagcgcg ctcggatcga gttgtagtgc ggtctcgtag agctccgctg cctgttcggg
     5881 gcgttgctgt cggctttcga gcaattgtgc tcggcggatg atcaatgccg cgcggtgctc
     5941 atcgctggac gccgacgcat tcgcaagctc cgcaagtgtc cggtccactt ccgaccacgt
     6001 ctgttttgcg aacgagcgct gttccgtcgc acgaaggatt gccgcatgcg agcggtcgag
     6061 cgccctcgcg gtgtgatacg cctcttccgc cgcgtcgcgg tcgccgagaa cgtcttcgag
     6121 caagcggccc ttcagcaagt acagcgtcgc cttgtcgcga ggctgccggg tgacacgtgc
     6181 ctccgcgtcg aatagcgcga gcgcatccgc aaacatccgg gctccgagca gcaaccggcg
     6241 tgctgcgcga atcgcgggga cgtagtcggg cgcgtgctcc agggccgctt ggtaatattg
     6301 aacggctcgc cgcgcgtcct tcagcggccc ctcgcaggcg cgcgccattt caaaatacaa
     6361 gcgcgccgcc ctccgcgcgt cgggctggcc gcgcagctcg gcctcgcact cctcaaccca
     6421 atctcgcgcc cgccgtgtgc gatcggcgcg actcgggctc ggggctcgct gtacctcagg
     6481 gcgaggcgga ggcggcagag tagggggacc gagggcgtcg ggttcttgga cgagatcgtc
     6541 ctgccctagc tcctcggtgg catctcggcg tagtgccgtg atctccccat tgtcgaaccc
     6601 catggtagct cgcatgctac cccggctgag ccaagatcgc atgggtccac atgaggtgag
     6661 tgaccaccct acatttgagc agggttttta ggccgcaggg gccaggtctc tcagctcctc
     6721 ggcaagcttc ttcttcgctc gcgcttcgag ctgacgcgcg cgctcgcggc tcacgccaag
     6781 ctctcggcca agggccgcca aggaggccgc gtcgtcactc aggattcgct tctcgacgat
     6841 gagacgctcg cgctcatcaa gcagggacat cgccccttcg agccgcatct gaatcccgct
     6901 ctgacgcctt tggcgggcaa cgacctggtc ctgcggttcg taggttcccg gaagcagctc
     6961 gacgagggtg gagtcggagt cctggtaagc aggtgcatcg agcgagatct ctctctggtc
     7021 gagccggcgc agcaggtccg tcatcttttc cttgctcgtc tcgaactcgg acgcgagcgc
     7081 ttcgatcaac tcgtccggat cggcgatcaa gttcgaaagg cgggcgcgct cgcgacgaag
     7141 tcgaaagaag agcttcgagc ggaatggtcc tgagcccgct cccaccatgg tgctcgagcg
     7201 aaccacggct tccaatacga aagcgcgaat ccaataaccg gcgtaagtca cgaagcgcgt
     7261 tccccggtgc ggatcgaact tgcgaaccgc agtcactagg ccgacgctgc cttcggcgat
     7321 caggtcgccg agcttgatgc catagcgtcg atattgaagc gcgatggcga tcacgtagcg
     7381 gaggttggct tcgacgaggc ggtccgaggc cttctgatca ccttcgagcg cgcgaagagc
     7441 gagctcgtgc tcctgttctc gggacagctt ggggatggcc cgaacctgtt ggatgtatcg
     7501 atcgagggag gatgatgact gcatggtgtt gtcctgtatg tgcgctctac cgttacgtta
     7561 cgttacaacc tggtgtcaag cactcgtgtc taactcttgt ctaggtagta gatgccataa
     7621 catagacaag gatgcaacag aaacaagtag gccccggagc gaaagttcgc ccaaggtcaa
     7681 gcaatcacga aatcattgag aaattaggtg gcgaagctcg agccgcatcc gcacgtctga
     7741 ctcgcctgcg gatttacaaa cttgaaccca gcgccttgca gcccttcgac gtagtcgatt
     7801 tcggttccct cgatgtactg ggcgctcatc atgtccacgt agatgggaat gccgtgcgag
     7861 gtaaacgtct ggtcgagctc gctcgtctcg tcctcgaaga acaaatcata cgaaaaacct
     7921 gcacaaccac caccgatgac gcgcactcta agcccttgct cggccaggcc ctctgcctca
     7981 cggatttctt tgactttgtc tgcagctcgg ggggtcaggt tcaacatgat gctccttgtg
     8041 ccgctactat aaggtcgagc agcgtttcgc gccagcaccg ggggttccgt agcgtcatgc
     8101 tgaggaaact cccagtcccg ggccaccagc gaccgggccg agtgcgcggg caggccggcc
     8161 ggcgaccgag aacccttccg gtccttgccc ttcgtggcca cgcttgagaa agccaagcgc
     8221 cagactgcgg tcgccctcgg tcaccgccga ggtgatctcg cccaccaccg tttcgccatg
     8281 caggatggga gtcgcggctt ctggcgggtc gcgcccttcg atcgcccagc gccacagcac
     8341 cttcggggcc tttccccggt tctgcagcat gaccacggtc tcttggccga tgtagcaacc
     8401 cttcttaaag gacacggcga gctcgttgag ccctgtttcc tgagggtagg tccagtcgcc
     8461 aaagtcgatc ccaaagagcg gtcgcccgag cagcacgtga gcgtaccgcc aggtcttctc
     8521 accaacgagg cctcctccga gcgcgtggca acgcgcgatc aggcgatcgc gctccgagct
     8581 cagggcgtcc gtgggaacga cccactgtcg accgccgaca cccagccgat ccgcaggcca
     8641 gcctccctcg ccgacttcgt gagcgagcgg tccgactgca gcgagtaggc cgaggtcggg
     8701 gcggtgctcg aggtcgacgt cctccatgat gatgtaccgt tccagacggg cgaggagcga
     8761 gtcgacctgc gccttcggca cgtcgagcaa gacctcgtag tcacccatga gcacgtagac
     8821 atccgcgagc gtccttccct tgagcgtcag cacgaacgca tacaccgaac cgccagcggg
     8881 cacgccttcg acctggtttg tgacctgtcc ctgtatccag tcgtgtgcat cgtcgccgcc
     8941 gaccgaaaca atcgcacgat cgtcaagggc gacgatgccc gcactttggg tcagccattc
     9001 gagctcgcgt gcctggtcca tgggcgagtt ctggtgcgcg aggcgcacgc tggcaagcgc
     9061 cggcctcaat ccgtgatcgg ctcgaggtga gccgagtgca gagacaggac tccagtttcg
     9121 gttcgcaccc gggcggcgcc gtcttcatcg atgccatcga acgagcccac cccatcctcc
     9181 catcgaactc gttcgccgag gagcgcgagc cgcgatcgaa gggcattcac cacgaccggt
     9241 gcgccgtctt gaatgaatcg cttcacccag cgctccatgt tcgagaggac cgcggcaaga
     9301 acttcggccc ggtcgaaggg ctcttcgacg ccgcgctccg cccgcaggga cgtggcggta
     9361 ccggccaagt cgggcggcca ttgcaatcga ttcacgttca ggcccactcc gatgatgacc
     9421 gagtcgatgc gcatgccgag tgtccggctt tcgacgagca cgcccgcaca cttgcgtctt
     9481 ccgatccaaa tgtcgttggg ccatttcacc cgtacagagc gttccggaag caagcccgca
     9541 accgcgtcgc gaacaccgag tccgcttgca agcgtcacga gcgcagtcga tgccggctcg
     9601 accgcaggcc tggccaccac cgagaagtag aggtctgaac ccggcggcga gacccaatgc
     9661 cggccatgcg cgccgcgacc ctgggtctgt tgatcggcca gcaccagatg cccatcggca
     9721 gcaccgttcg ctgccgccgc cgccgcgacg tccatggtcg aggcggtgct ctcgagcacc
     9781 tcgagggagc ggccccaagt tccgctgatt cgttcctgaa gccgctcagt ccgcaggtcc
     9841 tcgatcacgc gctagctcca gtcgaggccg agatcacaag atggagctga atgcgtcaat
     9901 gcaccgacgc tgatgacgtc gaccccaagc gcgcccaagc tcttgacgcg ttcgagcgtg
     9961 atgccgcccg aaacttcgac gatcgcccga cccgcgatcg aacgcagagc atcgcggacg
    10021 gcgtcgtcgt cgaagttgtc gagcatgatg atgtcggccc gggcggcgag tgcctcttgc
    10081 agttgggccg gcgtatcgac ctcgcattcg atgcggcagg tatgtggagc atacgcgcga
    10141 gcacgctcga tggcctcctt gcaaccacct gcggccgcga tgtggttgtc cttgatcaaa
    10201 accgcggctc ccaagtcctc gcggtggttg tgcccgccac cgcagcgcac ggcatatcgc
    10261 tcgaaggccc gcaaacccgg ggtcgtctta cgggtgtcgg tgattcgagt tggggagccc
    10321 tccggaagcg catcgacgaa tcgtcgcgtc atgctcgcaa ccccgctaag ccgttgcatg
    10381 aagttcagcg cgacccgctc gccgaggaga atcgaggctg cggggccgga gatctgagcg
    10441 agcgggctgc ctgcctccac gcggtcgccg tcctccacga gcgtgtccac gtctatcaag
    10501 aggtctaact gcgcgtagac ctcctcggcc accggtaacc cacagacgac gaggggctcg
    10561 cgtgcccgaa gggtggcagc cccgatctgg ttcggatcga cgcaggcctc cgtcgtcacg
    10621 tctccgcggt cgatgtcctc ggacaaggcg cggcgaacgg tttccgcgag aatggtcggg
    10681 gccggcggag tgacctgcat gtaggagtta tagccaactc cgcgccagaa tctcggaata
    10741 aagattgctc atcgcgtgaa gtgcgagcgc cgcgccaatc ccgccacgcc aggcgcgagt
    10801 ccatccgaag agcagtgcgg gaaaaaacac cgcgagccgc gcggggtgcg gctcgaccat
    10861 gaagtgaatt gccgcaaaga ggaccgcttg gagaagccat gcgcccggct cgagcgcaac
    10921 gccgagaacc cggagccgtt tcgcgcgaag gtcgctcagc gcggtctgca ggtaaccgcg
    10981 aaagaatgct tcctcgggta gggcgactac gatgagctgc gcgagaacga agctcggcag
    11041 gctggggggc aaggcgagcg agaacggctg ggcgggttga ttccaggcgt agaagccgag
    11101 cgcgtagatc ggaaacacga tcgccgccac ggcgaacgca aaaccgagct ccaccgcagc
    11161 cgaaggaaga gcgtcgacaa tcgcgcgacc gaggtcgaag agcccgagcg gtccttcggg
    11221 ccggtcgtcg gtgggtggct cgagcagccc tccgagcgag actccgtagt gcttgggttc
    11281 agcgcgtgtg aaccggatcg cggtcagtaa gaagatggcg gccaccaaga ggtgcacgta
    11341 tcggccgagc gctccgctcg attcgagccg cgtcaccgcg aagaccgacg cgcataccag
    11401 cgcatacacc cagaaaatct gtcgcaacgg tgcgtgcgcc tcggccatcg cgccacaact
    11461 acagcggcaa gcggcgcttg ccaagaagca ggcgacgcct acactctcgt cacctcgcag
    11521 ggacggcgag cacgggtgag gcgcggcggt gatttccgaa gacaagattg ctgagatccg
    11581 cgaccgggcc cccatcgcgg acgtgatcgg gaactacgtc gcgctcaaga agtcgggcaa
    11641 cagcctcaag ggcctttgtc ccttccacaa cgagaagtcg ccctcgttct acgtgcaccc
    11701 cagccggggc ttctaccact gcttcggttg caaggcctcg ggcgacatct tttcctttct
    11761 catgcacgtc gagggcaaga cgttccctga agtcgcgcgc gacctcgccg agcagaccgg
    11821 agtcgagctc ccggtttacg atccccagcg cgaggccgag ttccaacgga acaagcagac
    11881 gtcggaccgg ctcgcgtccc tcatcgagaa ggcgacccag ttttacgtcg cgcagttggc
    11941 agagcatccc gactcggcca tcgcgcgcaa cgagttcttg agccgcggca tctccgagga
    12001 gtctgcacgc gagtttcgtc tcggctacgc gccgcacggc tgggactcac tcgttcgctt
    12061 tctggagcag agcggagctt cgccagcgga cgcagaggcg gcagggctaa tcgtttcgcg
    12121 tcgctcgggt cgcggtcatt acgatcgatt tcgccatcgg ctcatgtttc ccatcgcgga
    12181 cgtgcacggc aaggtcgtgg cgtttagcgg ccgcgctttg actcccgctc cttcgatgga
    12241 agagcagaaa gagccgcctg caaaatacct caacagcccc gagaacccgc tgtatcacaa
    12301 aggcgaagtc ctctacggct tgcacgaagg tcgcgtcgag atccgacgtg aagccgcgac
    12361 agttctctgc gagggcaact tcgacctggt cgctcttcat caaggagggt ttcggaacgc
    12421 cgtggcaccg atgggcactg cgttcacaga agcgcatgcg aggttgctca agcgcttcgc
    12481 gcagaaggtc attctgctgt tcgatggtga caaggccggt cgcaaggccg tgcgcgaagc
    12541 ctacgcagtg gtcggcaagg ttggtctcga agctcacgtc gtcacgctgc cgcaaggcga
    12601 ggaccctgac agcttccttc ggcagcaggg ttcggaggcc ttgcgcgcac ggatcgtgag
    12661 cgcgcccacc attatcgaat atttaatcga cgatgcagca gcgacggcgg cgcccaaccc
    12721 ccctgcgaaa gccgatgcga tcgcaaaact tggtccgatt cttgccaagg tcgaaagccc
    12781 gatcgagcgc gggctctacg tcgagcgcgt tgcccgcaaa ttcgggatcg ctgacacggt
    12841 attggttcgt cgagagctgc gtcgaggcct gggtcgcggc ggcactcgtt cggtgccgag
    12901 tcgtgacgca gtgcagcagc cgattgcaac gccagtgtcg gagttacagt cgaagttgat
    12961 ttcggttctg atcgatcagc cgagcttatt gcatgaagcg agcgtgcaga agcttccgaa
    13021 gcttttgacg agccctgatc ttcgcgccat ctttgatctt atcttgcgga tggtggatat
    13081 gcagggatcg ctcgatgtca cggccttgct cgatgagttg cacgacaacc aactcaggcc
    13141 gtggttggaa gcgaagttgg cggtgcaaga gcacagcctg gaagacgcac gtcaaattct
    13201 cgacgatggt cttccactgc tcgaacgtaa aaacatcgaa catgagctgc cccgtttgca
    13261 gcaacgcatc gtcgaagcac gccgggtggg cgacgacgcg ttggcggcag ggctaactcg
    13321 agagtttgtg gagctaacgc gcagcgctca caggctcaaa tcgaacacta agcagaggtg
    13381 aagatggcga gcaagagctc agtgcgtgac gaggaatcga acgagtccaa gttcaagtct
    13441 gaaccagtgt cgcgcgtagt accgaaggtc aagggtcgcg aacgcaagga agtgcaggtc
    13501 cttctcgagc gcggccgaca acaaggtcat ctcacgtacg acgagctcaa cgaagctctt
    13561 cctccggaca tggtgacctc cgatcagatc gacgacctca tggtttggct gaagagcgaa
    13621 gacatcgacg tcgtcgatgg ggcgcccgaa ggtcagagcc ggacgagcga agtgccggtg
    13681 gccgccactc gaaccaccac gcgacgcccc tcggccattc ctccccccgc ggaggacagc
    13741 tacgcgcaga agtcgaacga cccggttcgc atgtacctcc gaaagatggg gtcggtttcg
    13801 ctgctcaccc gggaaggcga ggtcgaaatc gccaagcgca tcgaggacgg cgaagacaag
    13861 attttcgagg tgattttgaa ctcccgcgtg ggggtcgccg agatcttgga aatcggcgaa
    13921 agcctccgca gaggcaagct ccgcatcaag gatgtggttc gcgatctcga caaagaagaa
    13981 gaggtcgact tcgaagccgc caaagttcgt gcgctcaagg tgttcgacaa ggtgaagcgc
    14041 atcgagtcgt cgctggccaa agttcggaac gagctcgacg gcaagcgcaa ggtctcggac
    14101 cggagcaaga agcactacac cgcgcagctc gacaagcaca tacacgagcg gatcgctctg
    14161 ttgcgtgagc tgaatctcac caagaagcag atcgaccggg tggtctcgaa gatcaaggcg
    14221 tacacccgtc gcgtcgagcg cgcagagcgt gagatgaacg aggcagaggc gcgggtcggt
    14281 gggctcaagt acgaagacat gaagcgtgtg gtgcgcgaca tgcaggagag caaggccgtc
    14341 gagaagcgtg tgcagcggaa gtacggttcg gaagagcaaa tcctcatgtc cgaggagcac
    14401 atccgcgacg cccaaaaagc gatcgaccat gtcgaagaag acatggggca accggtcgag
    14461 cgtttgcgca acacgtacag ggcgctcgca gaaggtgaga ggcaatccga gcgagccaag
    14521 gctgagctcg tcgaagcgaa ccttcgtctc gtcgtgtcga tcgccaagaa atacacgaac
    14581 cgcggattgc agttcctcga cctgattcag gaaggcaaca tcggcctcat gaaggcggtc
    14641 gacaagttcg agtaccgccg aggctacaag ttctcgacct acgccacctg gtggatccgc
    14701 caggcgatca cccgggcgat cgccgatcag gcccgaacga tccgaattcc ggtccacatg
    14761 atcgaaacga tcaacaagct gatccgcacc tcgcgctacc tcgtgcaaga gctcggccgc
    14821 gagcccgatc ccgaagagat cgccgagcgc atggaaatgc cgctcgagaa ggtccgcaag
    14881 gtcctcaaga tcgccaagga gccgatcagc ctcgaaaccc cgatcggcga ggaggaagac
    14941 agccacctcg gtgacttcat cgaggacaag aacgccgtct ccccggtcga agccgtcatc
    15001 ggtggcaacc tcgaagacca gacacgccgc gtcctcaaga cactgacgcc ccgcgaggag
    15061 aaggtcctac ggatgcgctt cggcatcggc gaaaagagcg accacacgct cgaagaagtg
    15121 ggccaggact tcgaagtcac ccgcgaacga atccgccaaa tcgaggccaa ggccctccga
    15181 aagctacggc acccaagccg gtccaagcag ctccgaagct tcatggacac ctgatcgggc
    15241 cacggcagaa cacgacgcgg aggttcagcg gacggggcgc cttgcaaagg tggccggaag
    15301 gactgcgagc aggggccacg caaggcgccc ctcggagctc gcgccaatcg gagggggttg
    15361 ctccctgacc gattggcact cacacgccgc cgaacctccg cgtcgcgttc cgccgtggcc
    15421 ctccgtgcgg agacttcagc gtcagggtca gtgccagtgt cagcgctcgt gctccgacgt
    15481 gtataaagga gggcggctcg ggatgggcag cacagcgatt gggtcgagcc tagccgtagg
    15541 tcgtcttaag gatttggggg cgagtgggtc ccaatcgcga gcacgcccgt cgcgagccgt
    15601 cctccgctga ctccgtcgca tgctccagca cctttcattc gcgctccgct gcttatcaag
    15661 ctagaagtgc ttcaagggcc catagctcaa ttggttagag cccccggctc ataaccgggt
    15721 tgttcctggt tcgagtccag gtgggcctac tacgaaaatt cgtgctcttg cgccctccgc
    15781 gaaggcacgc tagagccccg ccagcttgtt gaactcggga ggaagtcttt gcgcgaagag
    15841 ctcgtagcgc ttgcgaaact ggccgaaatg gatgactcgg cgcgggacat cgatgccgag
    15901 ctgaagcaga ttccgcaacg attggaggag ctccggcata gcgttcagat gctcgagacc
    15961 atgctttcgc aggagcgaca gcagctcacg gaggccgagg ggctcagggc gcaacagagc
    16021 gcggagctca aggagcgagg cgaaggactt cagcgcgctc gaaagaagat cgcccagtca
    16081 tccagcatcc gcgaagccga cgctgcggaa cgcgaggtgg aagccaaccg ccgcgccatc
    16141 aaggagcggg aagacgagat cggtcgaatc gcgcaagcga tcgaagccaa gaaggcctcg
    16201 cttgcagagc gggaaaaaga cttcgaggac accaagagct cgttgcaggc tgacgaagag
    16261 acgtcgaaag cgcgcattgc ggagctcgag gagcagcgct ccaaactgct cgtcggtcgg
    16321 gacgagctcg ccgccaaggt ctcgaaaacg atcgtcaagc gttacgaacg cctgaagacg
    16381 ggcatcccca acgcggtcat catcatcacg gacgggaact gcccggcttg ccgtatggct
    16441 ttgcccgcgc agctcacgat tgacctgcgg cgcgcagacg agcttcacca atgcccgcac
    16501 tgccgccgga tcatcatcca caagagcgtc ctcgaggact gagcccacac accggggcgc
    16561 ggcggaacgc gatgcggggt tccgtcggac ggctcggctt gcaattgtgg ccggtgggac
    16621 ttgaggccgg gggccacgca agcctcgcct cggagctcgc gccaatcgga ggtgattgct
    16681 tcctgaccga ttgtcactcg ctacgccgc
//
LOCUS       DASRZF010000296        12996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_912278, whole genome shotgun sequence.
ACCESSION   DASRZF010000296 DASRZF010000000
VERSION     DASRZF010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 12996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12996
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912278"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /gene="hprK"
                     /locus_tag="VFG22_12910"
     CDS             <1..606
                     /gene="hprK"
                     /locus_tag="VFG22_12910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HET6417189.1"
                     /translation="AAPRSSRAIQAIHLVLDGILAPSQTRHGVLMDVHGIGTLLLGPS
                     GIGKSECALFLVERGHRFVADDQVILSLLPSEQVLGRAPPLLRNHLEVRGIGIINVRD
                     LFGANAVRSEKTVQLVVELCPWSDDEPFDRLGLDEATIDICGIPIPMLRIPVRTGRNM
                     AVILEVAARNHILKAAGHHGAQKFISTLMGDMEDPSSEPAQ"
     gene            603..1472
                     /gene="rapZ"
                     /locus_tag="VFG22_12915"
     CDS             603..1472
                     /gene="rapZ"
                     /locus_tag="VFG22_12915"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HET6417190.1"
                     /translation="MSEATQVVVVTGLSGAGRSTALRVLEDAGFFCVDNLPPALAPEL
                     LGLAGQDGKLGRVGFGIDVRTGGFLSGAEETLSELQRAGHLVQVIFLDCADDVLVRRF
                     SETRRPHALARTGDLHAAIAKERERLAGLRARADIVIDTTEFSVHDLRHHLIDYIGRD
                     AGRPSMVVRLVSFGFKYGVPVHADLVFDLRFLPNPHFVDALRPKTGMDPEVAAYVLEA
                     PETQELLRHLRPLLDFALPHYAREGKAYLTVALGCTGGRHRSVAMAEELARELGEGNE
                     VVVSHRDIGRVGP"
     gene            1469..1747
                     /locus_tag="VFG22_12920"
     CDS             1469..1747
                     /locus_tag="VFG22_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HET6417191.1"
                     /translation="MSEALSATVELVNPLGLHARAAAKLVDLANRYESHIEVSKGDQT
                     ADAKSIMGVLLLCGHQGAKIRFRASGPDAAAALDSLSALVAEGFGEVP"
     gene            1744..3444
                     /gene="ptsP"
                     /locus_tag="VFG22_12925"
     CDS             1744..3444
                     /gene="ptsP"
                     /locus_tag="VFG22_12925"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HET6417192.1"
                     /translation="MTRPMKLKGVAASIGVAVGPARVIGRERRRHPRRRLEESELPVE
                     RERFRQAVSKSRSEIETAKRDLTRKHGSTFAPILDVYLLMHEDALLIESISDTIRDER
                     INAEWAVSQVAERLKKPLLEDSSPYFRERARDVEHVCEHLLRHLSGERRDDAPADGPT
                     VLIAHDLSPADAVHLLAPPTIGLVTETGAGSSHTAILARTFGVPAVVGVGLSPIAIAD
                     DEMVVVDGFAGEVTVGASLDERHAAEARRDRFMAFLRAERSTPAVTRDGSPISVTANV
                     ELPSEVQAAIDNGAEGIGLYRTEFMCLDRPEPPTEDEQLDLYRSVVAAMAPHRVVFRA
                     FDWRGDKRLRAQDLKRHERGWLKTQIRAVLRASGEGAVAFMFPMVATLSEFLQYRALV
                     EECRTELGEEVAGFAPVPVGMMVEVPAAALLADRFAEHADFFSAGSNDLSHYTLAFDR
                     LDGSTRANPFEPAVLELLARTVKAANEAELPCSICGDMAADPVALGLVLGLGYRQVSV
                     PVRVLPLVRAIIRNLDLRVAAEVASEALDCASAEEVRELLVDRLGAELGALWRDQQIV
                     "
     gene            complement(3441..4022)
                     /locus_tag="VFG22_12930"
     CDS             complement(3441..4022)
                     /locus_tag="VFG22_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET6417193.1"
                     /translation="MTRRSRHEHDRDRASIRRVLVLSCMLAWSSAISPGCAHESTSPG
                     QTTSQAGRGPRLDLGIRLTDGRWLELLDLRGTPVLVFVFATFDAVSQASLKSLRPFVP
                     QHPDLVVVGVAAQPRAEQLVDAWAYALDPPFVVGADPYGRVENGESSLGKIEAVPTYI
                     LYDADGREVDRRTGLLSEGDLDRLVEPVMGDSN"
     gene            4061..4945
                     /locus_tag="VFG22_12935"
     CDS             4061..4945
                     /locus_tag="VFG22_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HET6417194.1"
                     /translation="MDGIRERLDQLLATCDAESTRRRDPVDFIHGYHEPRDQEIVGLI
                     AASLAFGNAVAARRSIERVLESLGAEPAALLIESDEAELRRRLHGFVHRIYRAEHLAS
                     VLSRAGRLLREHDSLGNAFVHFHRRSQGDFREGLSRFADALRGESTNRNLRHLVSDPR
                     AGSACKRLLLYARWMIRPSDGVDLGLWPIAPSELLIPVDTHIHRISRNLGLTKRRTAS
                     WATAEEITAVLRTFDPDDPVKYDFALCHLGVSRECPSRPDPSKCEQCVLQAVCSVWTD
                     KHARDRQRRKPVSFGSSA"
     gene            complement(4906..7491)
                     /locus_tag="VFG22_12940"
     CDS             complement(4906..7491)
                     /locus_tag="VFG22_12940"
                     /inference="COORDINATES: protein motif:HMM:NF039580.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HET6417195.1"
                     /translation="MSNMPELARRSPTPAPYSPNAIMRWLYRRFFAHIRVDENWSRGV
                     REAASKGVVVYVMRSLSFLDFICLDFLLKKFGLPLVRFVNDLGLWILEPFGKGERRLS
                     LRRQIPEGEALEKTVGDGYSALLFLRQPPQFGRRARRGRDLEVDLLRTLVAKQRTSAQ
                     PILLVPQTFIWSKQAGSASPGLLDLLFGPVQWPGRLRVLFQFLLNYRDAKLCSGEPFN
                     LQEFLRKNPELTDADAADKVRYALLRRMERDRAVIIGPMKKTTARIQDELLRSPRLRS
                     HIQRYADEKGISYAKAETVARKQLQRLCAKQSIFVVDLMHRFLTWVWSKMYDGVVLDR
                     EGLERVREAARDASLVLLPSHKSHIDYLVLSDMLYGHAMMPPLIAAGDNLSFWPIGVL
                     LRRGGAFFIKRSFHDDSLYPELVEAYIRKLIAEGFPIEFFLEGGRSRTGKALPPKYGL
                     LSMVYDSAAKLRGTKVKLVPISIGYERIIEERSFVHELSGGDKQSENVGDLIKSSSVL
                     RSKWGRLYVQFGEIIDFDEFQEQSIRRVGERISHPDASPTGLKRSAVRALAHKIMYSI
                     NEVTVVTPAALVATALLSHQKRGMTRTSLLTACQDLLLTLDVENARIAHQLRIGDERI
                     REDTIDEALRLFLDARLIVSHDTGPEAIYTIDSERRLALEYYQNTIIHFFVPRALISA
                     ALLVNKEQWAQISRLNDRVQQLSRLFKHEFIYRTDATFNDIFQDTLQEMVRVGEIELR
                     DETAQATEGVMRQRVERYALMLQTFFESYLLALRGAEIVLDGPIPKKDWYKRTLALGQ
                     QMYLAGEIERRESLSKLKLETALKALEDYRLIQLNGDTLERGDGVESVADLHALEPKL
                     TGFLR"
     gene            complement(7576..8148)
                     /locus_tag="VFG22_12945"
     CDS             complement(7576..8148)
                     /locus_tag="VFG22_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417196.1"
                     /translation="MRIPIQTGNGDRLGFRRVRAPSALLAFTLLFAAGSAHAQTGAIL
                     CTLTENGAPARGTLEAEQSGKEVGSGSCGDPLAVPPGKYRVSVTLDGALDTPTKTADV
                     TVTAGKTARLTIDFKTGVLEVRVETKAQGGTAQITVMRGSERIGTVGSSVPARLSAGA
                     YGVVVRLGGQERRFDVDLRPGQRRLVRAQF"
     gene            8313..8741
                     /gene="rplM"
                     /locus_tag="VFG22_12950"
     CDS             8313..8741
                     /gene="rplM"
                     /locus_tag="VFG22_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HET6417197.1"
                     /translation="MATQSAKASEIERKWYVVDAAGLPLGRLASKVAHVLRGKHRPEY
                     TPHVDTGDFVIVINAAQVRLTGNKLANKYYHRHSGYPGGLTSESYGELIKRKPEFVIE
                     RAVKGMLPKNSLGRQMFRKLKVHAEGTHPHVAQQPVPFPL"
     gene            8760..9167
                     /gene="rpsI"
                     /locus_tag="VFG22_12955"
     CDS             8760..9167
                     /gene="rpsI"
                     /locus_tag="VFG22_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HET6417198.1"
                     /translation="MTHSKVIDGRNYGTGKRKNAIARVFLSAGAGNVTVNGRTFENYF
                     PREILRIVAMQPFAALSKQGQFDVEVNVSGGGVSAQATAVRHGIARALVGMDETNKPT
                     LKKAGYLTRDARKKERKKPGQPGARKKFQYSKR"
     gene            9273..10325
                     /gene="argC"
                     /locus_tag="VFG22_12960"
     CDS             9273..10325
                     /gene="argC"
                     /locus_tag="VFG22_12960"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HET6417199.1"
                     /translation="MTSKPIRTIVVGGTGYTGAELVRLILGHPSLELVSIVGNSTAGQ
                     NVSDVLPSLRGIVDGAVEPFEPDAIAKHADAVFCALPHSASAEAVSALRQRGTVVLDL
                     SADFRFADLAVYESWYGKHPVPERVPEAVYGLVELHREELRNADLIAVPGCYPTASTL
                     ALAPLLKNALVRLDGIVVDAKSGVSGAGRGVSSTTHFPETAEGVRAYKAGVHRHGPEI
                     EQELSRVAGLPVRITFVPHLLPMTRGILATCYAQASGPDVSAAACVESARAFYAGSPS
                     VHVLDVGEHPDTLWVRGSNRVHLGYTQDPNSGTLIAMSAIDNLVKGASGQAIQCLNVR
                     FGLDEAEGLKAPAQWP"
     gene            10316..11221
                     /locus_tag="VFG22_12965"
     CDS             10316..11221
                     /locus_tag="VFG22_12965"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET6417200.1"
                     /translation="MAVTKVLHVSDVHLEDGFAGVPISSFFNKRIVGLANLQFRRRKV
                     FLDAVQKVEALAEFSKEQGVDLVICTGDYTALGTEPEIRYARKIIEPLTHAPLGFFTV
                     PGNHDLYLRDTVEAGWFDQHFGSFMRTELPEYAVDGVWPQVWFPADGLALIGINSARA
                     NPKPTYSSGRIPDEQLDALVRILDDPRLFDRFVMIATHYAPRLASGRPDRPAHGLENA
                     DELLAISAAARRGVIVHGHIHWRYHLRVPETPLSLCCAGSTTHEGREGLWMFEVGENG
                     VTATPGRWDQTRYVLEHEQRVDIDP"
     gene            11260..11883
                     /locus_tag="VFG22_12970"
     CDS             11260..11883
                     /locus_tag="VFG22_12970"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6417201.1"
                     /translation="MAARQFGAKPEARRAVRRLVDLECEVYSELWGEAIAHRVTDVSE
                     EGLWIETDLLLEIGTEITLTFYPPDWEEPLCVAGSIQRVELQRRPSGGASVGMGVAFE
                     ALRTDERRRLTQSMRSLRAQEAFILDQRTLTGVPVGVGEAWAPVSDPSRTVAGWAAPS
                     PRAGYAGRPSRITATPVKSAEDSEASKGAFSGGIDLAASVFSTSRLD"
     gene            complement(11880..>12996)
                     /locus_tag="VFG22_12975"
     CDS             complement(11880..>12996)
                     /locus_tag="VFG22_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417202.1"
                     /translation="LARPRPAAAMQRLESLWNRGWTERLWLLIAALALGTCFTFGLLC
                     LLLDLRIASIAVRVGRVAFFVPFVLAVLFFGLPWLRFAFGRFGTFRFFPALAGGLAIG
                     AFVALSFQPALSAHFSPKAIYDAYTELSEGKPEPLASYDLPSTSAHYYTSAAIEQIDA
                     PNELIEFLGKPGQRWVVMEAEQLPKLDRAYRQETGEHLNVADARNARLLLIAARPIEG
                     RSNESFLAHAILNEAPVPQHRVGANYDDVVELIGYDLELPRGDSVGAGQHFGVTWYWR
                     VLGKVPTGYEVFVHIDAHGLRLNGDHIPVAGRYPTKLWEEGDVIADSQELMVPANFRT
                     GDYVMYVGLFSGSKRLEVKSGPNDGVNRVEAGTLPIR"
BASE COUNT         2355 a         3999 c         4192 g         2450 t
ORIGIN      
        1 gcagcacccc ggtccagccg tgcgattcag gcgatccatc tcgtgctcga tggaattctc
       61 gcgccttccc aaacccggca cggggtgctg atggacgtgc atggaatcgg gaccttgctt
      121 ctcgggccga gcggcatcgg gaagagcgaa tgtgcgttgt ttctcgtcga acggggccat
      181 cggttcgtgg cggatgacca ggtgatcctc tccttgcttc cgtccgagca ggtgcttggg
      241 cgcgcgccac cgctcctgcg aaatcatctc gaagtgcggg gcatcggaat catcaacgtc
      301 cgcgacctct tcggggccaa tgccgtgcgg tccgaaaaga cggttcagct cgtggtcgag
      361 ctctgccctt ggagcgacga cgaacccttc gatcggctcg gcctcgatga agccacgatc
      421 gacatctgtg ggattcccat tcccatgttg cgaatccccg tccgtacggg gcgcaacatg
      481 gcggtcatct tggaggtcgc cgcgcgcaac cacatcctca aggcggcagg tcatcacggc
      541 gcacagaaat tcatcagcac cttgatgggc gacatggagg acccgagctc ggaacccgcg
      601 caatgagtga agcaactcag gtggtggtcg tcaccggact ctcgggcgca ggcagaagca
      661 ccgcgcttcg ggttctcgag gatgctgggt tcttttgcgt cgacaatctg ccacccgcgt
      721 tggcgccaga gctcctcgga ctggcgggcc aggacggcaa gctcggaagg gtcggattcg
      781 gcatcgacgt tcgtaccggc ggcttcttga gcggcgcgga ggaaacgctc tccgagttgc
      841 agcgcgcggg tcacttggtg caggtgatct tcctcgattg cgcagacgac gtgctcgtcc
      901 gacggttcag cgaaacacgg agaccacatg cgctcgctcg aacgggggat ttgcacgcag
      961 cgatcgccaa agagcgtgag cgtcttgcgg ggcttcgcgc gcgcgccgac atcgtcatcg
     1021 acaccaccga attcagcgta cacgacttgc ggcatcatct gatcgactac atcggacgcg
     1081 acgcggggcg tccttcgatg gtggtgcgct tggtttcctt tggtttcaaa tacggtgtgc
     1141 ccgtgcacgc tgatctcgtg ttcgatcttc ggttccttcc gaacccgcat ttcgtcgatg
     1201 ccttgcgccc caagacagga atggaccccg aggtcgctgc ttacgtgctc gaagcccctg
     1261 aaacgcagga gcttctccgg cacttgcgac cactgctcga ctttgcgctc ccgcactacg
     1321 ctcgcgaagg caaggcctac ctcacggtgg cgctcgggtg cacgggcgga aggcatcgct
     1381 ccgtggcgat ggccgaggag ctcgcccgcg agctcggtga ggggaacgag gtggtggtct
     1441 cccaccgcga cataggacga gtcgggccgt gagcgaagcg ctcagtgcca cggtcgagct
     1501 cgtcaatccc ctgggcctgc atgcgcgagc ggccgcgaag ttggtcgacc tcgcaaaccg
     1561 ctatgaatcg cacatcgagg tgagtaaagg cgatcagacc gcagacgcca agagcatcat
     1621 gggggtgctg cttctctgtg gccatcaggg ggcgaagatt cgctttcgag cgtccggacc
     1681 cgacgccgcc gccgcgctcg attcgctttc agctctggtc gccgagggct ttggggaggt
     1741 tccatgactc ggcccatgaa gctcaaaggg gtcgccgcct cgatcggcgt cgccgtcggg
     1801 ccagcgcgcg tcatcggtcg tgagcgtcga cgtcacccac gcagacgctt ggaagaaagt
     1861 gaattgcctg tcgagcgaga gcgattccga caagcggtct cgaagtcgcg ctccgaaatt
     1921 gaaacagcca agcgggatct cacgcggaaa cacggctcga cgttcgcacc gattctcgac
     1981 gtctacctat tgatgcacga ggatgcgctg ctgatcgaat cgatctccga cacgatacgc
     2041 gacgagcgga tcaacgccga atgggccgta tcgcaagtgg cggagcgtct caagaagccc
     2101 ttgctcgaag acagctctcc atactttcgg gagcgcgcgc gtgatgtcga gcacgtttgc
     2161 gagcatctgc ttcgtcatct ttcgggcgag cgtcgtgatg atgcaccggc cgatggtccg
     2221 accgtgctga tcgcacacga tctctcaccc gcggatgcgg ttcacctcct cgcgccgccc
     2281 accattggct tggtcaccga gacgggtgca ggtagcagcc acaccgcgat tcttgcgcgc
     2341 acgtttggcg tgccggccgt ggtcggcgtc ggtttgtcgc ctatcgcgat cgccgacgac
     2401 gagatggtgg tggtcgatgg gttcgccggc gaggtcaccg tgggcgcttc cctcgatgag
     2461 cgtcacgccg ccgaagctcg ccgcgatcga ttcatggcgt tcctccgcgc cgagcgctcg
     2521 acgcccgccg tgacccggga tggatctcca atctcggtca cggccaacgt ggagcttccg
     2581 agtgaagttc aggcggccat cgacaacggc gcggaaggca tcggtctgta ccgaaccgag
     2641 ttcatgtgtc tcgaccgtcc cgagccaccg accgaggacg agcagctcga cttgtatcgg
     2701 agcgtcgtcg cggccatggc tccccaccga gtcgtttttc gagccttcga ctggcgaggc
     2761 gacaagcgtc tgcgtgcgca agacctgaag cggcacgagc ggggctggtt gaagacacag
     2821 atcagagcgg ttctgcgtgc gagcggggag ggcgcggtcg cattcatgtt cccgatggtc
     2881 gccacgctct ccgagttcct tcagtaccgc gccctggtcg aggagtgcag gaccgagctc
     2941 ggcgaggaag tcgccggatt cgcgccggtt ccggtcggga tgatggtcga ggtacccgct
     3001 gccgctttgc tcgcggatcg atttgccgaa cacgccgatt tcttctcggc cggaagcaac
     3061 gacctctcac actatacgtt ggccttcgac cgtctagacg gtagcactcg ggccaatccg
     3121 ttcgagccag ccgtgctcga gcttcttgcg cgcaccgtca aagcggccaa cgaagcggag
     3181 ttgccctgct cgatctgcgg cgacatggcc gccgacccgg tcgctctggg cctggtcttg
     3241 ggtctcggtt accgacaggt ctccgttcct gtgcgagtcc ttcccttggt gcgcgcaatc
     3301 attcgcaatc tcgatctccg cgtagcggcc gaagttgcat ccgaggcgct tgattgtgcc
     3361 tccgctgagg aggttcgtga gcttctcgtc gatcgcctcg gcgccgagct cggcgcgctc
     3421 tggcgagacc agcaaatcgt ttagttcgaa tcgcccatga ctggctcgac gagtcggtcc
     3481 aggtcgcctt cgctcagcag acccgtgcga cgatcgactt cacggccgtc ggcatcgtaa
     3541 agaatgtagg tcggcaccgc ttcgatcttt ccgagtgagc tctctccgtt ctcgacgcgt
     3601 ccgtacgggt cggcgcccac cacgaacggc ggatcgagtg cataggccca agcgtcgacc
     3661 aactgctctg cgcgcggctg agccgcgacg ccgacgacga cgagatcggg gtgttgcggc
     3721 acgaacggcc gaagtgactt gagcgatgcc tggctcacgg cgtcgaacgt tgcgaagacg
     3781 aaaacgagga ccggggttcc gcggaggtcc aggagctcga gccatcggcc gtccgtcagg
     3841 cgaattccga ggtcgagacg tgggccgcgc ccagcttgcg acgtggtttg tcccgggctc
     3901 gtcgattcgt gcgcacagcc cggcgagatc gcggaggacc aggcgagcat gcaactgagc
     3961 acgagcactc gtcgaatcga ggctcggtcc cggtcatgtt cgtggcgact ccgtctggtc
     4021 attgcagtag gctcggcaat atagcaagga cttcagcgaa gtggacggta tccgcgaacg
     4081 cctcgaccag ctgctcgcta cctgcgacgc agagtctacc cggcgcagag atcctgtcga
     4141 tttcattcac gggtaccacg agccccggga tcaggaaatc gtcggcctca tcgcagcgag
     4201 cctcgccttt ggcaatgccg tggcggccag acgaagcatc gagcgggtgc tggaatcctt
     4261 gggtgcagag cctgccgcgc tgctgatcga atccgacgag gccgagcttc gtcgccgcct
     4321 ccacggtttc gttcatcgga tctatcgcgc ggagcatctc gcgtcggtgc tttcgcgagc
     4381 aggacggctg ttgcgtgagc acgactccct cggcaatgcg ttcgttcatt tccaccgaag
     4441 gagccaaggt gatttccgtg agggattgtc tcgctttgca gatgcgcttc gcggcgaatc
     4501 gacgaaccgc aacttgcggc atctggtcag cgatcccagg gcaggcagtg cgtgtaaacg
     4561 cctgctgctc tacgcacgat ggatgattcg cccctcagac ggcgtggacc tcggcctctg
     4621 gcccatcgcg ccttccgagc tgctgattcc cgtcgacacc cacatccatc gcatcagccg
     4681 aaatctgggc ctcacgaaac gacgcacggc gagttgggcc acggccgagg agatcaccgc
     4741 cgtgctccgg accttcgacc cggacgaccc cgtcaaatac gactttgccc tctgtcacct
     4801 cggcgtttcg agggaatgcc caagtcgccc cgacccgagc aagtgtgagc aatgcgtctt
     4861 acaagcggtc tgcagtgttt ggaccgacaa acacgcgcgc gatcgtcagc gaagaaagcc
     4921 cgtgagcttc ggctctagcg cgtgaaggtc ggccacgctt tcgacgccat cgccgcgctc
     4981 gagggtgtca ccgttgagct ggatcagtcg atagtcttcc agcgccttga gggccgtctc
     5041 gagcttcaac ttcgaaagcg actcgcgacg ctcgatctcc ccagcgagat acatctgctg
     5101 ccccaaggcg agagttcgct tgtaccagtc cttcttggga atcggcccgt cgaggacgat
     5161 ttcggcaccg cgaagtgcga ggaggtaact ctcgaagaag gtctgaagca tgagggcgta
     5221 tcgctcgacc cgttgccgca tcacgccttc ggtggcctgc gcggtctcat cgcgcagctc
     5281 gatttcgccg acgcgcacca tttcctgaag cgtgtcttgg aagatatcgt tgaacgttgc
     5341 atcggttcgg tagatgaact cgtgcttgaa aagcctcgag agttgctgaa cgcggtcgtt
     5401 tagacggctg atctgggccc attgctcctt gttgaccagc agcgcagccg aaatgagagc
     5461 ccgcggaacg aagaagtgaa tgatcgtgtt ctgatagtat tcgagcgcca gtcggcgttc
     5521 cgaatcgatc gtgtagatcg cctctggccc cgtgtcgtgc gagacgatga gacgcgcgtc
     5581 gagaaacagg cgtagcgctt cgtcgattgt gtcttcgcgg attctctcgt ctccgatgcg
     5641 aagctggtgt gcgatccgcg cattctcgac gtccaaggtg aggagcaagt cctggcaagc
     5701 ggtcagtaga gaggtcctcg tcatcccgcg cttctgatgg ctcagcagtg cggtggccac
     5761 gagggccgcc ggggtgacga ccgtgacctc gttgatcgag tacatgattt tgtgcgccag
     5821 tgctcggacg gcggagcgtt tcaagccggt gggactcgca tcgggatgcg agattcgttc
     5881 accgacgcgg cgtatcgatt gctcctggaa ctcatcgaag tcgatgattt cgccaaactg
     5941 gacgtagagg cgaccccact tggagcgcag gacgctcgag ctcttgatca aatcgccgac
     6001 gttttccgac tgcttgtctc cgccggaaag ctcgtggacg aacgaacgct cctcgatgat
     6061 gcgctcgtag ccgatcgaga tcggaaccaa tttgaccttg gtgccgcgga gcttcgcggc
     6121 ggaatcgtag accatcgaaa gcaggccgta cttgggagga agcgccttgc ctgtgcgcga
     6181 gcggccgcct tcgaggaaga attcgatcgg aaacccctcc gcgatcagct tgcgaatgta
     6241 agcctcgacg agctccgggt agagcgagtc gtcatggaag ctccgcttga tgaagaaggc
     6301 gcccccgcgt cggagcagga ccccgatcgg ccagaagctg aggttgtcgc cagccgcgat
     6361 gagcggaggc atcatcgcat gtccgtagag catgtcgctc aggacgaggt agtcgatgtg
     6421 gctcttgtga ctcggaagga gcaccagcga ggcgtcacgc gccgcttctc gaacgcgctc
     6481 gaggccctct cggtcgagca cgacgccgtc gtacatcttc gaccagaccc aggtgagaaa
     6541 ccggtgcatg aggtccacga cgaagatgct ctgcttcgcg cagagccgct ggagctgctt
     6601 gcgcgcgacg gtttccgctt tcgcatacga gatgcccttt tcgtctgcgt atcgctgaat
     6661 gtgagacctg agccgcgggc tgcgcagaag ctcgtcctga atgcgcgcgg tcgtcttctt
     6721 catcgggccg atgatgacgg cacggtcgcg ttccatgcgg cggagtaggg cgtagcggac
     6781 cttgtcggcg gcgtcagcgt ccgtgagctc ggggttcttt cgaaggaact cctgcagatt
     6841 gaagggctct ccggaacaga gcttggcgtc tcggtagttc agcaagaact ggaacaagac
     6901 tcggagccgg cccggccact gcacggggcc gaagagaaga tcgagcagac cggggctcgc
     6961 cgagccggct tgcttgctcc aaatgaacgt ctgcggcact agcaagatcg gttgcgcgga
     7021 ggtcctttgc ttggcgacca gcgtccggag caggtcgacc tcaagatcgc gtccccgacg
     7081 cgctcgtcgg ccaaattggg gcggctgccg caagaagagc agagcgctgt acccatcgcc
     7141 gacggtcttc tccaaggcct cgccttcggg tatctgccgg cgcaagctca ggcgccgttc
     7201 ccccttgccg aagggctcga gaatccagag ccccaaatcg ttgacgaagc gaaccagcgg
     7261 caggccgaac ttcttcaaga ggaagtcgag gcagatgaag tccaggaacg agagcgagcg
     7321 catcacgtag accacgacac ccttgctcgc ggcctcgcgc acgccacggc tccagttctc
     7381 gtccacccgg atgtgggcga agaagcgccg gtagagccag cgcataatcg cgttgggtga
     7441 atacggcgcc ggcgttggtg aacgccgcgc cagctccggc atgttcgaca tccctgagtc
     7501 agaccctagc agcgtgcaag atcaggtcga gtatacgcag ttccgctttt attccggctt
     7561 ttggcggaat cgccgctaga actgggcgcg gacgaggcgg cgctgcccgg gccgcagatc
     7621 gacgtcaaag cgtcgctcct ggccgccgag ccggaccacg accccatagg cgcccgcgct
     7681 gaggcgtgcg ggaacgctac tgccgacggt acctatgcgt tccgagccgc gcatgaccgt
     7741 gatctgtgct gtgccgcctt gagctttcgt ctcgactcgc acttcgagaa cgccggtctt
     7801 gaagtcgatc gtcagccgcg cggtcttgcc tgctgtcacg gtgacatctg cagtcttggt
     7861 tggagtgtcg agggccccgt ccagcgtcac gctcaccctg tacttgccgg ggggcactgc
     7921 gagtggatcg ccgcaggatc ctgagcctac ttccttgccc gactgctccg cctcgagggt
     7981 gccgcgggcc ggcgcgccgt tttcggtcag ggtgcagagg atcgccccgg tttgcgcgtg
     8041 tgccgacccg gcggcaaaga ggagcgtgaa ggccagcagt gccgacggag cccgaacccg
     8101 tcggaacccg aggcgatcgc cattgcctgt ttgaatagga attctcaccg cttaccagtt
     8161 tatcgcaatt cgaggccgga gtgggggctc gttcgaaatc ctttcgaaaa cccaagcttt
     8221 ccggccgcaa gtatcacgcc ctgcctgctt gacaccgatg gggccggcgg ctacgaaacg
     8281 cgccccgaaa cggggtctta ggagcgaatc tcatggctac ccagagtgcc aaagcgtctg
     8341 aaatcgaacg caaatggtat gtcgtcgatg cggcgggtct tccgcttggc cgtttagcgt
     8401 ccaaggtggc gcacgtcctt cgtggcaagc accgtccgga gtacaccccg cacgtcgata
     8461 cgggtgactt tgtgatcgtc atcaatgcgg cccaggtgcg gctcacgggc aacaagctcg
     8521 cgaacaagta ctaccatcgt cactccggct atccgggcgg cctcacctcg gagagctatg
     8581 gcgagttgat caagcgcaag ccagagttcg tcatcgaacg cgcggtcaag ggcatgctcc
     8641 cgaagaacag cctcggccga cagatgttcc gcaagttgaa agttcatgcc gaggggacgc
     8701 atccccacgt cgcccagcaa cccgtgccct ttcccctttg atctcgagag atttggttaa
     8761 tgacacattc taaagtcatc gacggacgca actacggaac cggaaagcga aagaacgcga
     8821 tcgcgcgcgt ctttctgagc gccggcgcag gcaacgtgac cgtcaatggt cgcacgttcg
     8881 agaactactt cccgcgggag atccttcgga tcgtcgcgat gcagcctttt gccgcgctct
     8941 cgaagcaggg ccagttcgac gttgaagtca acgtatcggg cggaggcgtg tccgcgcagg
     9001 ccacagccgt tcggcacggc atcgcccggg ccctcgtcgg catggacgag accaacaaac
     9061 cgacgctcaa gaaggccggt tacctcaccc gcgacgctcg taagaaggag cgtaagaagc
     9121 caggtcagcc gggcgcccgc aagaagttcc agtactcgaa gcgctgagaa cctcgaatcg
     9181 caaatctcga gccgcggcgc cttctgtgcc gcggcttttt tgcttaatgc catagacaac
     9241 gcacatgtca gtatcaggat gtagttgagc gaatgacctc gaagcccata cgcacgatcg
     9301 tggtcggagg gaccggttac accggggccg agctcgttcg tttgatcctg gggcaccctt
     9361 cgctcgagct ggtgtcgatc gtgggcaact cgacggcagg ccagaacgtg tccgacgtgc
     9421 tgccgagcct tcgtggcatc gtggacggag cggtcgagcc tttcgagccc gacgcgattg
     9481 cgaagcatgc ggacgcggtg ttttgcgcat tgcctcattc ggccagcgcc gaggcggtga
     9541 gcgcgttgcg acaacgaggg accgtagtgc tcgatctctc cgcggatttt cgatttgccg
     9601 acctcgccgt ttacgagagc tggtacggca agcaccccgt ccccgagcgc gtgcccgagg
     9661 cggtgtatgg cctcgtcgag ctccaccgcg aggagctgcg caacgcggat ttgatcgcgg
     9721 ttcccggttg ctacccgacc gcatcgacct tggcgctcgc gcccttgctg aagaacgcgc
     9781 ttgtccgact cgatggcatc gtggtcgatg caaagagcgg tgtgtctggt gcggggcggg
     9841 gtgtttcgag cacgacgcat ttcccggaga cggccgaagg ggtccgcgcg tacaaggccg
     9901 gcgtgcatcg gcatgggccc gagatcgagc aagagctgag ccgggtcgcc gggttgccgg
     9961 ttcggatcac gttcgttcct caccttcttc cgatgacgcg agggattctc gcgacctgct
    10021 acgcgcaagc gagcggaccc gatgtgagcg ctgcggcgtg cgtcgaatcc gcgcgtgcgt
    10081 tctatgcggg ctcaccttcg gtgcacgtgc tcgacgttgg ggagcacccg gacacgctct
    10141 gggtgcgggg gtccaaccga gtacatctcg gctatacgca ggacccgaac agcgggaccc
    10201 tcatcgcgat gagcgcgatc gacaacctcg tgaagggcgc atcgggccag gccattcagt
    10261 gcctcaacgt tcgttttggc ctcgatgaag ccgaagggct caaggcgccc gcgcaatggc
    10321 cgtaaccaag gttcttcacg tcagcgacgt tcacctcgaa gatgggtttg ccggggtgcc
    10381 gatcagcagc tttttcaaca agcggatcgt cggtctcgcg aatctgcagt tccgtcgccg
    10441 gaaggtcttc ttggacgcgg tgcagaaggt cgaggcgctg gcggagtttt cgaaagagca
    10501 gggcgtcgac ctcgtgattt gcacggggga ttacacggcg ctcgggaccg aacccgagat
    10561 ccgatacgcc cggaagatca tcgagccact cacccatgcg ccgctcggct tcttcacggt
    10621 gcctggcaat cacgacctgt accttcgtga caccgtcgag gccggttggt tcgatcagca
    10681 tttcgggagc ttcatgagaa ccgagcttcc ggagtacgcg gtcgacggag tctggccgca
    10741 agtatggttt ccggctgacg gcttggcgct gatcgggatc aacagcgcgc gtgccaatcc
    10801 gaagcctacc tattcgagcg ggcggattcc cgatgaacaa ctcgatgcgc tcgtgcggat
    10861 tctggatgat ccccgtctgt ttgatcgctt cgtgatgatc gccacccact atgcgccgcg
    10921 cctcgcgagc ggtagaccgg accggccggc gcacggcctc gagaacgcgg acgagctctt
    10981 ggcgatcagt gcggcggctc gacgcggcgt gatcgtccat ggtcacattc attggcggta
    11041 tcacttgcga gtgcccgaga ctccgctcag cttgtgctgt gccgggagca cgacgcacga
    11101 gggccgagag ggcctttgga tgttcgaagt cggcgagaac ggggtgacgg cgactcccgg
    11161 ccgatgggat caaacccgct acgtgctcga gcacgagcag cgcgtcgaca tcgacccgta
    11221 atttgccgcg cgagggccgc ctcgaataca agcggaggta tggcagctcg ccagttcggg
    11281 gcaaaacccg aggcccgcag ggccgttcga cggctcgtcg atctcgaatg cgaggtctac
    11341 tccgagcttt ggggggaagc gattgcgcac cgggtgaccg acgtgagcga ggaagggctt
    11401 tggatcgaga ccgatctcct tctcgagatc ggaaccgaga tcacgctcac gttttatccg
    11461 cccgactggg aagagccact gtgcgtcgcc ggtagtattc agcgcgtcga gctccagaga
    11521 aggccgagcg gaggggcttc ggtcgggatg ggcgtcgcgt tcgaggcttt gcgaaccgat
    11581 gagcgccgcc gtctcacgca gagcatgcgc tcattacggg cgcaggaggc gttcattctc
    11641 gatcagcgga cgctcacggg ggttccggtc ggggtggggg aggcatgggc gccggtttcc
    11701 gatccgagcc gaaccgtcgc cggttgggcc gctccttcgc cacgcgcggg gtacgccggc
    11761 cggccctcac ggatcaccgc gactccggtg aagagcgcag aggattctga agcctccaag
    11821 ggcgccttca gcgggggcat cgacctggct gcgtccgtgt tctcgacctc gagattggac
    11881 tagcgaatcg gcagtgtccc ggcctcgacc ctgttcacgc cgtcgttggg acccgacttc
    11941 acctcgaggc gcttgcttcc actgaagaga cccacgtaca tcacatagtc gccggtgcgg
    12001 aaattcgcgg ggaccatcag ctcttggctg tcggcgatga cgtctccctc ttcccagagc
    12061 ttggtcgggt aacgcccagc gaccgggatg tgatctccgt tgaggcgaag gccgtgcgcg
    12121 tcgatgtgca cgaacacctc gtaacccgtc ggcaccttgc cgagcactcg ccagtaccag
    12181 gtcacaccga agtgctgacc ggcgccgacc gagtcccctc gcgggagctc gagatcgtat
    12241 ccgatcagct ctacgacgtc gtcgtagttg gcacctaccc ggtgctgagg cacgggcgct
    12301 tcgttgagga tcgcgtgagc gaggaagctc tcgttcgagc gtccttcgat tgggcgcgcg
    12361 gcgatcaaaa ggagccgggc attgcgcgca tcggccacgt tgaggtgctc gccggtctct
    12421 tggcgatatg cacggtcgag cttgggcagt tgctcggctt ccatcacgac ccagcgctgt
    12481 ccgggcttac cgagaaactc gatgagctcg ttcggggcgt cgatctgctc tatcgccgcg
    12541 ctggtgtagt agtgggcaga cgtggacggc agatcgtacg aggccagtgg ctcgggtttc
    12601 ccctcgctga gctcggtgta ggcatcgtag atcgccttgg gggagaagtg tgcgctgagc
    12661 gcgggctgga acgagagcgc gacgaatgcg ccgatcgcca accctccggc gagcgccgga
    12721 aagaaccgga atgttccgaa tcgtccgaag gcgaaacgca gccaggggag tccgaagaac
    12781 agcacggcca agacgaacgg cacgaagaag gcaacgcggc cgactcgaac ggcgatcgac
    12841 gcgattcgaa gatcgagcag caggcagagc aaaccgaatg tgaagcaggt gccgagcgcc
    12901 agcgccgcga tcagaagcca gagccgctct gtccatcctc tgttccagag ggactccagc
    12961 cgttgcatcg ctgcggcggg gcgaggccgt gcgagc
//
LOCUS       DASRZF010000297        10658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_5034654, whole genome shotgun sequence.
ACCESSION   DASRZF010000297 DASRZF010000000
VERSION     DASRZF010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 10658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10658
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5034654"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1524)
                     /locus_tag="VFG22_12980"
     CDS             complement(<1..1524)
                     /locus_tag="VFG22_12980"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HET6417203.1"
                     /translation="MTHIVVDPVTRIEGHLRIEAEINDGQVSDAWSSSTMFRGIEIIL
                     QGRDPRDAWAFTQRICGVCTTVHAIASIRAVEDAIGAKPPPNARILRNLIIASQCIQD
                     HVIHFYHLHALDWVDIVSALEADPKETATLAQSISDWGKSSATYFKGIQDRVKGLVER
                     GQLGPFANAYWGHPAYKLPPAANLMAVAHYLEALEWQREFIKMHAILGGKNPHLQSFL
                     VGGMATPVDPDRQASLNIQTIAQFKKLIAGAQEFVSKVYIPDLLAVASFYKDWAGYGA
                     GVGNYMVYGEYPLDDQADPERYIKSGIIRARDISKVEEFDPSKITEQITHSWYNYEGG
                     EEQALHPSEGETKPNYTGPEPPFERLDTAKKYSWLKSPRYDGVPMEVGPLARMLVNYV
                     QGHEQVQGLVNHVLKTLGVGPEALFSTLGRVAARGIETQLLVDKIGDWVDELADNMGK
                     GELRIHDNSKWDPESWPADCMGAGFHEAPRGSLGHWVHVKDAKIARYQCVVPSTWNAG
                     "
     gene            complement(1521..2651)
                     /locus_tag="VFG22_12985"
     CDS             complement(1521..2651)
                     /locus_tag="VFG22_12985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HET6417204.1"
                     /translation="MDEPGNDFRRELEARGVSRREFMGFCSAMAVMFGLPKGATAMIA
                     DALENKPKPTLVWLNFQDCAGNTESFLRASKPTAAEVILDLISIDYHEVIMAAAGHQA
                     EANLHSVVENQKGAYIAVVEGSIPTGANGAYCTIGGKSAYQIANEVCGNALATVAVGT
                     CASFGGIPAASPNPTGALGVSDAVPGIKTLINLSACPVNAQNLTGTLVYFLTYGRFPA
                     LDKLNRPLFAYGKSIHDNCERRAHYDSGQYVEQWGDEAHRDGHCLYKMGCKGPVSFQN
                     CPNVGWNDNTSWPIACGHGCIGCAEPDFWDKMTPFYSHLPGFPGFGKGSDIDKVGFWA
                     TIGVTTAFATHTLVHLGKKLVRSGADQAPDGPPTRTDEGGAE"
     gene            2871..4154
                     /locus_tag="VFG22_12990"
     CDS             2871..4154
                     /locus_tag="VFG22_12990"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HET6417205.1"
                     /translation="MSEESWRAAGDYRFFEVVQGYIEQAARLTQLPSHVKAILAEPKN
                     ELIVHFPVRMDDGDTRVFKGYRVQHNNVMGPYKGGMRYHQDVTLDELKALATQMTWKC
                     ALLGVPFGGGKGGVKFNPRDHSRDELRRITRRFTHALGSNIGPSHDIPAPDMGTNAQT
                     MVWMMDTYINTGPTYEKNDQRAVVTGKTINAGGSEGRESATGQGVVHCIKEWARENRF
                     DLEGATAIVQGFGNVGSHTAVLLAKLGVSTIATGDHSGYLRNDEGFNPHKLQDYVQAN
                     GSLEGYPRGDKISREEFFQTKADLFIPAALENQVGAQEASELDVRLIAEGANGPINPD
                     GEAVLEDRGIPVIPDILANSGGVTVSYYEWVQNRNSEHWDAEEVQRRLLHMMRRAYQR
                     TMFYATEKKVHPRVAAYALALESLATSYEERGIFP"
     gene            4243..5157
                     /locus_tag="VFG22_12995"
     CDS             4243..5157
                     /locus_tag="VFG22_12995"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HET6417206.1"
                     /translation="MAAEAVFQGMIPAEGVEDLLGGLEQVGTSGLLSFQSESGSGTVR
                     LLHGQIADAAASADEERALEILLSLRGGEFAVYPQLPHLPVSRGTDTTRRGSLAVHPP
                     ADLMRYCENAGLTGRLLLEHRGRLAIGYYEKGELSDVSIDGGSAADFVLALEWTEGTF
                     RVDAVPPPRETLPPTLRDKAPSAIPKNPTALPLVQAFEEGLAALGRADDTPVSGLRPQ
                     IDDRDEDATIRIIRRPGEEELEEPSELWDALDYSPSFATESATTLSVKSKDDGRPIGF
                     VRTLGWVGAVFLVVAGCLWLLASLPPLE"
     gene            5168..6781
                     /locus_tag="VFG22_13000"
     CDS             5168..6781
                     /locus_tag="VFG22_13000"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HET6417207.1"
                     /translation="MSPRLRPRRWLLSLIASFIMLVPRIAGAQAPDRGEPYRPGDQVS
                     GPEPEVERETEGDDEELRDPYGSPSEARRADPISSQVRYVLEGIVVVGNSRTKAKVVR
                     KFIPLKQGDFLDPESPELLATEWRLMGTGWFDAVDIRLERGAEHGYVVLVVEVKERNT
                     VVIEQLVAGLSKGVTETGNNEESLFPWLGFKVTETNLAGRGIRLSGTALLSGFQQGGR
                     LDLRYPKLIKDEYGVRFGTFFLNGREFYGVDPLVSKPCGEPTCPGSSTVEHAVVRYRR
                     GGFMVGIGKDFTTTLRYSLDWVGDIVSVLNRPEAASEPRGNDIVPIDFAIQDGKSFVS
                     SLRFALVFDKRDDPGITKEGVVFRGVVTVGTRFLGSDYDYLELEAWVRRWWRLPWNHT
                     IRLGAFGGAAFGSTPFFYLFHISDMTDLIPSRFMEMQLDAQRPPNLLGTSINNNHLGD
                     LAWRIDIGYNVPVYERVRVNGIREINLYTLFGIFGLADLRDPRAGVNGYSGFSRFPVD
                     LTLDVGFRFDTRVGVFQVGFSTVLGFIRL"
     gene            6778..7371
                     /locus_tag="VFG22_13005"
     CDS             6778..7371
                     /locus_tag="VFG22_13005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417208.1"
                     /translation="MRYGVVALVLLMAAAVFAQAGLPIRDLDVIWSRGSPKVSFTVKD
                     LADARVREELSSGLRKRLVVTVGSHLRGSNWRMAVREFSCDVTRDLWEDGYLVRIGTR
                     SERLKTLEDVLDRCLVVEGLFAGEPKSFEPHRGKEIYFAVQAEFNPISKKQCRELIRP
                     TSGDDPIGPITVNIVRRRICQAERRVEFRSVYSKVPQ"
     gene            7368..8882
                     /locus_tag="VFG22_13010"
     CDS             7368..8882
                     /locus_tag="VFG22_13010"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6417209.1"
                     /translation="MSRFERKILFAIGLSVFLTLGGSIFLAQGALREVYQVGVNPRFG
                     AELVHGVEARRGQLMALREGSEQVADAVKWAVEAELRKSRRTPAFEPILHALLERYEV
                     VQGIRISDDSGRVLARVVRPSDPAGGELRTTTRDRETDSAAGTLLISVEVAVPEVYFQ
                     RLQEAGEAADVYSRLQRQGASVSDVYVWVFGFMAFLVIVVAFLIGAIVSRRVTRRVMD
                     LADAAHQVGAGDLTVALPTGATDEVAELTNAFNQMVRDLRDSRTRIEYLQRIGAWQDF
                     ARRLAHEIKNPLTPIQLAAQELDETYGGEDAAYREKLKHARAIIEEEVATLRRLVGEF
                     SAFAKLPQADLSPADLCELMYGIEDSIPAMLEDLGRGSPAPVEVSVSCATEPIRVRMD
                     PMMLKRGIDNVVRNAIQAVYSENPSGGGRVTVRAYTRRGSAVIEICDNGPGVAEENWD
                     RVFDPYYTTRTEGTGLGLAIVKKVVLEHDGEVRVDRAPQGGARFTIELHLMESR"
     gene            8879..9424
                     /locus_tag="VFG22_13015"
     CDS             8879..9424
                     /locus_tag="VFG22_13015"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET6417210.1"
                     /translation="MNEPTQSRSGKWFRWFMTAMVFVALYAWMTRGNASPLMGERAPD
                     LSMPIAAGEISGTPSKVSLSDLGGQVVVLEFWASWCGACKRTTPILNELKAEFAEQAV
                     VFYGVNVEPIDRQRLQAAHTAFGAEFPSLHDRTGTVQRRFAIEMLPTVIVVGPDGVVR
                     WASTGLPSKMRLRGAISEALM"
     gene            9522..10277
                     /locus_tag="VFG22_13020"
     CDS             9522..10277
                     /locus_tag="VFG22_13020"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HET6417211.1"
                     /translation="MVQALQGPSLEERVAQLQALLQAADKDMTREYQERFDVAWIYHD
                     SALEGVVYSMDELNAAIYGSEVADPALIPVYDEIRNHMGAIEMIREMSSRRRFTPNLE
                     VIKKLYAQLAPEELEGKGPPKYRKDMPLHRLYFHEISQPDKISYRMRQLVQWLSDPET
                     KKTMHPVRMASKAQYQLLHIYPFPRHSGKVARLVMNLILMRAGYPPVVIHSTERQRYY
                     EALRSGEDATATLVNQALGAAIETAIQYFEEAP"
     gene            complement(10282..10650)
                     /locus_tag="VFG22_13025"
                     /pseudo
     CDS             complement(10282..10650)
                     /locus_tag="VFG22_13025"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
BASE COUNT         1999 a         3207 c         3361 g         2091 t
ORIGIN      
        1 tcctgcgttc caggtgctcg gaaccacgca ctggtagcgg gctattttgg catctttcac
       61 atgaacccag tgcccgagcg agcctcgtgg cgcctcgtga aagccagccc ccatacaatc
      121 ggcgggccag ctttccgggt cccacttgct gttgtcgtgg atgcggagct cgcccttgcc
      181 catgttgtca gcgagctcgt cgacccagtc gccgatcttg tcgaccagca gctgtgtttc
      241 gatgccccgt gccgcgaccc tgccgagcgt ggagaacaag gcctccggcc cgacgcccag
      301 cgtcttgaga acgtggttga ctaggccctg gacctgctcg tgaccttgaa cgtagttgac
      361 caacatgcga gccaagggcc cgacttccat cggcactccg tcgtagcggg gcgacttcag
      421 ccaggagtac ttcttggcgg tgtccagacg ctcgaaaggt ggctccggac cggtgtagtt
      481 gggcttggtc tctccctcgg acgggtggag cgcttgctcc tcgccgcctt cgtagttgta
      541 ccaagagtgt gtgatctgct cggtgatctt cgaaggatcg aactcttcca ccttcgagat
      601 gtcacgagct ctgatgatgc ccgacttgat gtagcgctcc ggatcggctt ggtcgtcgag
      661 cgggtactcg ccgtacacca tgtagttgcc gacaccggcg ccgtatccgg cccagtcctt
      721 atagaacgaa gcgaccgcaa gaagatcggg gatatagact ttactcacga actcctgtgc
      781 gcccgcgatc agcttcttga actgagcgat cgtctggatg ttcaatgaag cctgtctgtc
      841 cgggtcgacc ggagtcgcca tgccgccgac caggaagctc tgcagatgtg ggttcttgcc
      901 gccgagaatg gcgtgcatct tgatgaactc acgctgccac tcgagcgctt cgaggtagtg
      961 ggcgaccgcc atgaggtttg ccgcaggcgg tagcttgtac gcgggatggc cccagtatgc
     1021 gttggcgaac ggtccgagct gacctcgctc gaccagcccc tttacgcgat cctgaatacc
     1081 cttgaagtaa gtcgcgctcg acttgcccca gtctgaaatc gactgcgcga gcgtcgcggt
     1141 ttccttcgga tcggcttcga gcgccgacac gatgtcgacc cagtcgagcg cgtgcagatg
     1201 atagaagtgg atcacgtggt cctggatgca ctgggacgcg atgatcaggt tgcggaggat
     1261 ccgcgcgttg ggcggcggct tcgcgccgat cgcgtcttcg accgcacgga tcgaggcgat
     1321 cgcatggacc gtcgtgcaga cgccgcagat gcgctgggtg aatgcccaag catctcgcgg
     1381 gtcacggcct tggagtatga tttcgatacc tcggaacatg gtcgacgagg accaagcgtc
     1441 agagacttga ccgtcgttga tctccgcttc gatgcgcaga tggccttcga tgcgggtcac
     1501 cgggtcgaca acgatatggg tcattctgcc cctccttcat cggtccgcgt cggcggtccg
     1561 tcgggtgctt ggtccgcacc gcttctaacg agtttctttc cgagatgaac caaagtgtgg
     1621 gtagcaaacg ccgtcgtgac gccgatggtc gcccagaagc cgaccttgtc gatgtctgat
     1681 cctttgccga agccagggaa gcccggcagg tgcgaataga acggcgtcat cttgtcccag
     1741 aagtcgggct ccgcgcagcc gatgcagccg tgaccgcaag cgatgggcca gctcgtgttg
     1801 tcgttccaac cgacgttggg acagttctgg aaagacacgg ggcccttgca gcccatcttg
     1861 tagaggcagt gcccgtcgcg atgcgcttca tcgccccatt gctcgacgta ttggccggag
     1921 tcgtaatggg cgcgcctctc gcagttgtcg tggatcgact ttccgtaggc gaaaagcggg
     1981 cgattcagct tgtcgagcgc tgggaaccgt ccgtacgtca ggaagtacac caaggtccct
     2041 gtgaggttct gcgcgttgac cggacaagcc gagaggttga tcagcgtctt gatgcctgga
     2101 accgcgtcgg acacgccgag cgcgcccgtc gggttgggcg aggcagcagg gatgccgcca
     2161 aacgatgcgc aggtgccgac cgccaccgtc gcgagggcgt tgccacagac ttcgttggcg
     2221 atttggtacg ccgactttcc accgatcgtg cagtaagcgc cattcgcgcc ggtcgggatc
     2281 gagccctcga ccactgcgat gtatgcgccc ttctggttct ccaccaccga gtgcaagttt
     2341 gcctccgcct ggtgtccggc agccgccatg atcacctcgt ggtagtcgat cgagatcaga
     2401 tcgagaatca cctcggccgc agtcggcttg ctggcacgca ggaacgactc cgtgttgccc
     2461 gcgcaatcct ggaagttgag ccacaccaaa gtcggcttcg gcttgttctc gagtgcgtca
     2521 gcgatcattg ccgtggcgcc cttcggtaaa ccaaacatca cggccatcgc agagcagaag
     2581 cccatgaact cgcgacgtga cacgccacgg gcctccaact cacgacggaa gtcgtttccc
     2641 ggttcatcca tcgcatcctc ctcgcggttc gatagcaaag gctgcagtag taattctatc
     2701 agcggcccag tgagcaggac tttaccgcca cggaacctcg aaaaacaagg ttctgatcgc
     2761 tgttgagaac cgttttccct agcaggcgcg ttcgttgcgc cgatcggcgc agtcgcgcaa
     2821 tttttgcgcc gatcttggtg tccagaaacg cacgcgctag catcgtgtcg atgtcggaag
     2881 aatcatggag ggcggcgggc gactatcggt tcttcgaagt cgttcaaggt tatatcgagc
     2941 aggccgccag gctgacgcag ctcccgagtc acgtgaaggc gattctcgcg gaacccaaga
     3001 acgagctcat cgttcacttt cccgtgcgga tggacgatgg cgacactcgc gtgttcaagg
     3061 gttaccgagt tcagcacaac aacgtgatgg gtccgtacaa gggcgggatg cgctaccacc
     3121 aagacgtcac cctcgatgag ctcaaggcgc tggcgaccca gatgacctgg aagtgtgcat
     3181 tgctcggcgt gccttttggc ggcggaaaag ggggggtcaa gttcaacccg cgcgatcact
     3241 cccgtgacga gcttcggcga atcaccagac ggttcaccca cgcgctggga agcaacatcg
     3301 gcccttcgca tgacattccg gcgcccgaca tgggcaccaa tgcccagacg atggtctgga
     3361 tgatggacac ctatatcaac acgggcccca cctacgagaa gaacgaccag cgagcagtcg
     3421 tgaccgggaa gaccatcaat gcgggcggct ccgaagggcg cgagtccgct acggggcagg
     3481 gtgtagtgca ctgcatcaag gagtgggccc gggagaatcg cttcgacctc gaaggtgcga
     3541 ctgcgatcgt tcaaggcttc ggcaacgtgg ggtctcacac cgcggtcctg ctggccaagc
     3601 tcggtgtttc gaccatcgcg accggcgacc attccggata cctacgcaac gacgagggct
     3661 tcaatccgca caagttgcag gactacgttc aagccaatgg gtcgctcgag ggttacccgc
     3721 gcggagacaa gatcagtcgc gaggagttct tccagaccaa ggccgatctc ttcatcccag
     3781 cggccctcga gaatcaggtc ggggcacaag aggcgagcga gctcgacgtg cggctgatcg
     3841 ccgaaggcgc gaacggtccg atcaaccccg acggcgaggc cgtactcgaa gatcgcggga
     3901 tcccggtcat tcccgacatc ctcgcgaatt ccggcggtgt gaccgtcagt tactacgagt
     3961 gggttcagaa caggaacagc gagcactggg acgctgaaga agtccagcgt cgactgcttc
     4021 acatgatgcg acgcgcctat caacgcacga tgttctacgc gaccgaaaag aaggtacatc
     4081 cgcgagtcgc ggcgtacgcc cttgcgctcg agagcctcgc gacctcgtat gaagagcgcg
     4141 gcatcttccc ctgatttatc tggaaattct ggagtttacg ggcgctcctc ggaccccggg
     4201 gcgccttgac aattggggcc ccgggaatca ctccactcag tcatggccgc cgaagcagtc
     4261 ttccaaggaa tgatccccgc agagggcgtc gaggacttgc tcggcgggct cgaacaggtg
     4321 ggcaccagcg gcttgctttc gtttcaaagt gagtccggat cgggcacggt tcggctcctt
     4381 cacggccaga ttgccgacgc tgcggcatct gctgacgaag aacgtgcgct cgagatcttg
     4441 ctctcgctgc ggggcggcga gttcgcggtc tatccgcagc ttccgcacct gccggtctcc
     4501 cgcggcaccg acaccactcg gcgaggctcg ctcgcggtgc accccccggc cgacctgatg
     4561 cgctactgcg agaacgctgg cctcaccgga cggctcttgc tcgagcaccg tgggcgcctc
     4621 gcgattggat actatgaaaa aggagagctc agcgacgtga gcatcgatgg cggaagcgcc
     4681 gccgatttcg tcctcgcgct cgaatggacc gagggcacgt ttcgtgtcga cgccgtgccg
     4741 ccgcctcggg aaaccctgcc gccgactctt cgcgataagg ccccgagtgc aatccccaag
     4801 aacccgaccg cgcttccgct ggtccaggcg ttcgaggaag gcctcgccgc gctggggcgc
     4861 gccgacgaca caccggtttc cggactgcgc ccgcaaatcg acgaccgcga tgaggatgcc
     4921 accatccgga tcatacgcag gccgggcgaa gaagagctcg aggagccgtc cgagctctgg
     4981 gatgcgctcg actattctcc ctccttcgca acggaatctg ctacaactct ctccgtgaag
     5041 tcgaaggacg acggtaggcc aattggtttc gtgaggactc tgggatgggt cggggcggtt
     5101 ttcttggtgg tggccgggtg cctttggctt ctcgcgtcac tcccgccgct ggagtagtct
     5161 cggcgtcgtg agcccgcgct tgcgaccgag acgctggctt ctgagcctga tcgcctcctt
     5221 catcatgctc gtccctcgga ttgcaggcgc tcaggcgcct gatcgaggag agccgtatcg
     5281 ccccggcgac caggtctccg ggcccgagcc cgaagtcgag cgagagaccg agggcgacga
     5341 cgaagagcta cgcgatccct atggcagtcc gagcgaagcg cgccgggccg atccgatctc
     5401 gtctcaggtt cgctatgtgc tcgaagggat cgtcgtcgtc ggaaacagtc ggacgaaggc
     5461 gaaggtcgtt cgcaagttca ttcccttgaa gcaaggcgac ttcctggacc cagagtcacc
     5521 cgagctgctc gccaccgagt ggaggctcat gggaaccggc tggttcgacg ccgtcgacat
     5581 tcggctcgag cgaggtgccg agcatggata cgtggtcttg gtggtcgagg taaaggagcg
     5641 caacaccgtc gtcatcgaac agttggtcgc gggcttgtcg aagggcgtga cggagacggg
     5701 caacaacgaa gaaagcctct tcccttggct cggcttcaag gtgacggaaa ccaatctcgc
     5761 cggccgcgga attagactct cgggaacggc gctgctcagc ggcttccagc agggcggacg
     5821 cttggatctg cggtatccca agttgatcaa ggatgagtac ggcgtccggt tcggaacgtt
     5881 cttcctcaac ggacgtgagt tctacggagt cgatccattg gtcagcaaac cttgcggcga
     5941 gccgacctgt ccaggaagct cgacggtcga gcacgccgtg gtgcggtatc gacgcggcgg
     6001 tttcatggtt gggattggca aggatttcac gaccacgctc cgctacagcc tcgattgggt
     6061 gggcgacatc gtgagcgtcc tcaaccgccc cgaagcagct agcgagccgc gtggcaacga
     6121 tatcgtcccg atcgacttcg cgatccagga tggaaagagc ttcgtgtctt cgcttcgttt
     6181 cgccctcgta ttcgacaagc gggacgatcc tggaatcacc aaagagggcg tcgtgtttcg
     6241 cggtgtcgtc acggtgggca cccgctttct cggtagcgac tacgactacc tcgagctcga
     6301 ggcctgggtt cgacgctggt ggcgacttcc ctggaaccac acgatccggc tcggcgcctt
     6361 cgggggagca gcgttcggaa gcacgccttt cttctatctg tttcacatct ccgacatgac
     6421 cgatctgatt ccatcgcggt tcatggagat gcaactcgac gcgcaacgcc caccgaatct
     6481 cttgggcact tcgatcaaca acaatcacct gggtgacctc gcgtggcgaa tcgacattgg
     6541 ctacaatgtt ccggtctacg agcgcgttcg cgtcaacggt atccgcgaga tcaatctcta
     6601 caccttgttc ggcatcttcg gactcgccga ccttcgagat cctcgcgcgg gcgtgaatgg
     6661 ctactcgggt ttttctagat ttccggtcga tctcaccctt gatgtcggtt tccgcttcga
     6721 cacccgggtg ggggtctttc aggtcgggtt ctctacggtc ctggggttca tacggctgtg
     6781 agatacgggg tcgtagctct ggtcttgctc atggccgctg cggtattcgc gcaggcaggg
     6841 ctacccatcc gcgacctgga tgtgatctgg tcacgaggtt cgcccaaagt ctcgttcact
     6901 gtgaaggacc tggcggacgc gagagtccgc gaggagcttt cgagcggtct tcgcaagcgc
     6961 ttggtggtga cggtgggctc gcacttgcgg ggctccaact ggcggatggc cgtacgagag
     7021 ttcagttgcg acgttacacg cgacctttgg gaggacggct atctcgtccg cataggcact
     7081 cgcagtgaac gcctgaagac gctcgaagat gttctcgatc gctgcctcgt ggtcgaggga
     7141 ctcttcgccg gcgaacccaa gagctttgag ccgcatcgag ggaaagagat ctacttcgcc
     7201 gtgcaggcgg agttcaatcc gatttccaag aagcagtgca gagagctgat ccgaccgaca
     7261 tccggagacg atcccatcgg tccgatcacc gtgaacatcg tgcgtcggcg tatctgccaa
     7321 gccgagcgcc gggtcgagtt ccgaagcgtc tattcgaagg tgccgcaatg agccggttcg
     7381 agcgaaagat tctgttcgcg atcggcctgt cggtctttct gacactcgga ggcagcattt
     7441 ttctggcgca aggcgctctg cgcgaggtgt atcaggtcgg tgtaaacccg cgatttggag
     7501 ccgagctcgt tcacggggtc gaggcccggc gcgggcagtt gatggcgctc cgagagggct
     7561 ctgagcaggt ggccgatgcc gtgaaatggg ccgtcgaggc cgagctgcgg aagagccgaa
     7621 ggacgcccgc tttcgagcca atcctccacg cacttctcga acgatacgag gtcgtacaag
     7681 ggattcgcat ctccgatgat tcgggtcgag ttctcgcacg ggtggtccgg ccgtcggatc
     7741 cggcgggcgg agagctgcgc accacgacgc gggatcgaga gactgacagt gcagctggga
     7801 cgctgctgat ctccgtcgaa gtggccgttc cggaagtcta cttccagcgc cttcaagagg
     7861 ccggggaggc tgccgatgtg tattcgcggc ttcagcgtca gggcgcctct gtctccgacg
     7921 tctacgtttg ggtcttcgga ttcatggcgt ttctcgtcat agtcgttgcg ttcttgattg
     7981 gcgcgatcgt atcgcgacgc gtaactcgtc gcgtgatgga cttggcggac gcggcccacc
     8041 aagtcggtgc aggagatctc accgtcgctc tgcccaccgg cgcgaccgac gaggttgcgg
     8101 agctaaccaa tgccttcaac cagatggttc gcgacctgcg cgattcgcgc actcgtatcg
     8161 agtatttgca gcgcatcggc gcctggcagg atttcgcacg ccgcctcgcc cacgagatca
     8221 agaacccact cacccccatt cagttggccg ctcaggagct cgacgaaacc tatggtggag
     8281 aggacgcggc atatcgggag aagctcaagc acgcccgggc gatcatcgag gaggaggtgg
     8341 ctacgcttcg ccggctggtg ggcgagttca gcgcatttgc caagctcccg caggcggatt
     8401 tgtccccggc ggacctctgt gagctcatgt acggcatcga ggattccatc cctgcgatgc
     8461 tcgaggactt ggggcggggg tccccagcgc ccgtcgaggt gagcgtgagt tgtgcaacgg
     8521 agcccatccg agtgcgtatg gaccccatga tgctcaaacg cgggatcgac aacgtcgttc
     8581 gaaacgccat tcaggcggtc tattctgaga atccttcggg cgggggccgc gtcaccgtcc
     8641 gcgcgtacac gcggaggggc tcggccgtca tcgagatttg cgacaacggt cccggtgtcg
     8701 ccgaagagaa ctgggatcgg gtattcgacc cctactacac gacgaggacc gaaggcaccg
     8761 ggctcggctt ggccatcgtg aagaaggtcg tactcgagca cgatggagag gtgagggttg
     8821 accgggcgcc ccagggcggg gcgcgtttca cgatcgagct tcacttgatg gagtcacgtt
     8881 gaacgagccg acgcaaagcc gctcgggcaa gtggtttcgt tggttcatga cagcgatggt
     8941 cttcgtcgct ctctatgcct ggatgacccg cggcaacgca tctccgttga tgggggagcg
     9001 ggctccggat ttgagcatgc ccatcgctgc cggagagatt tcagggacgc cgagcaaagt
     9061 cagcctgtcg gacttggggg ggcaagtcgt cgtcctcgag ttctgggcga gctggtgtgg
     9121 cgcgtgcaaa cggacgacgc cgatcctcaa cgagctcaag gcggagttcg ccgagcaggc
     9181 ggtcgtgttc tatggagtga acgtcgagcc gatcgatcgc cagaggttgc aagccgcgca
     9241 cacggcgttt ggggccgagt tccccagcct gcacgaccgt acaggaacgg ttcagaggcg
     9301 attcgcgatc gagatgctgc cgactgtcat cgtggtcggc ccagatgggg tcgttcgctg
     9361 ggcctccacc ggcttgccga gcaaaatgcg gctccgcgga gcaatttcgg aagccctcat
     9421 gtagccggat tcggtcatgg ttgacgagtt gggggcccgc tgttaggttc gccggccgcg
     9481 cggggcggat gaggccaact ccgcaggcga gagggatatt cttggttcaa gcgctgcaag
     9541 gtccgagctt agaagagcgt gtcgcccaac tgcaggcgct gctccaagcc gcagacaagg
     9601 atatgactcg cgagtatcaa gagcgcttcg acgttgcgtg gatctaccac gacagcgcgc
     9661 tcgagggtgt cgtttactcg atggacgagt tgaatgcggc gatctacggc tcagaagtcg
     9721 cagatcctgc gctgatcccc gtctacgacg agatccgcaa ccacatgggc gccatcgaaa
     9781 tgatccgcga gatgtcctcg cgtcgtcgct tcacgccgaa cctcgaggtc atcaagaagc
     9841 tgtacgcgca gctggcaccc gaagagctcg aagggaaggg ccctcccaag taccgcaaag
     9901 acatgccgtt gcatcggctc tacttccacg agatctctca gcccgacaag atcagctatc
     9961 gaatgcgtca actcgtccaa tggctttccg acccggaaac caagaagacg atgcatccgg
    10021 tgcgcatggc gagcaaggcc cagtaccagc tgcttcacat ctatcccttt ccgcgacaca
    10081 gcgggaaggt tgcgcgcctg gtgatgaacc tgatcctgat gcgtgcgggc tatccaccgg
    10141 tcgtcattca ctcgaccgag cgccagcgat actacgaggc gctgcgaagc ggcgaggatg
    10201 ccaccgcgac gctcgtgaat caggcgctcg gtgcggcgat cgagaccgcc atacagtact
    10261 tcgaagaagc cccttgaggc cctaggaagc gctgcctaga aagatgggca atgcggagat
    10321 cgcggccaca tagcgagcgc cggctcggag ggcttccgaa gcgttctccg gggtaatccc
    10381 tccgatggcc acgactgggc gtgacaccgt ctgggacacg gtttcgaggc tcttcagacc
    10441 caccaccgga tctggatttt ccttggttct cgtcgcaaag accggaccaa aggcgatcag
    10501 gtcagctccg tctgcatcgg ctcgtacagc ctgatcgatg tcgtgagtag agcggccgat
    10561 ctacatttct cctacgacac atcgggcctc tctcacggaa agatcgtcct ggcccagatg
    10621 caagccatct gcgtggacga gccgcgcgat gtcagcgc
//
LOCUS       DASRZF010000298         3060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_223495, whole genome shotgun sequence.
ACCESSION   DASRZF010000298 DASRZF010000000
VERSION     DASRZF010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223495"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="VFG22_13030"
     CDS             <1..575
                     /locus_tag="VFG22_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417212.1"
                     /translation="SAAEGPATAANAQQDEGTPRDLAAEIEAALGIPSDCVQDFQAAE
                     PRTIRVQVSATVRPSGVIISPSASGAGLSPRERQCIAARVGAISLRPLGEDVSRRVST
                     EIAIEYSPPASPSPSLGVPEPQLRNVRDPLPQGPDIAPSGRPIGDREGRPIPEPNARP
                     IQDRSSRDTRSRKPRAIDGHEIDDGAEEWR"
     gene            complement(579..2531)
                     /locus_tag="VFG22_13035"
     CDS             complement(579..2531)
                     /locus_tag="VFG22_13035"
                     /inference="COORDINATES: protein motif:HMM:NF018000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417213.1"
                     /translation="MPFRPFDRARFAIERWLVRGAFHRVLLAAVLVALISLTGGVLAL
                     VLGRGFGELPEAVWWSFLRLSDPGYLGDDTGLVNRTLSTVLTVLGYVVFLGALVAIMT
                     QWLDARMAKLESGLTPVARDGHILILGWTNRTDAIIRELLLSQGRARRFLDRRGGPGL
                     HIVLLAETVTAAFVQNLRDAVGPAWDERRVTIRSGSALRPEHLARVDAAHAAAILIPG
                     ADFGDKSLQSDTQTIKTLLSLDALSCGEERPPRVVAEVFEARKVHLARKAYRRDIEVI
                     SSDGVISRLLAQNIRHPGLSRVYDELLTHREGNEIYIREADELLGLEIEDLHRYFPNA
                     VLMGLVRPSGGAFAPLLNAGPGTRLEEGDKLVVLAHSYVDSKPSDSPSLARLPRETSD
                     TRTNTERRDTRHILVLGWNHRVPALLEEFGTYGDERYVVRIGSKVPIARREQLLSHRP
                     FDASSLAITQLEVDFTDLDDIEALGPELADSIVFMGSDWMESAEEADARTLFGSLIVD
                     ERLRERSTEATVILELLDPDNASLVTRSRGELIVSPLVVSHLLTQVALRPELQSVFDE
                     LFTAGGAEITFHPVTDYADSGRLTFADLERSAAACGQTALGVRTRSSGSVLLNPPKDT
                     LLEEPADLDLVALVTMSYPTAARVPG"
     gene            2634..>3060
                     /locus_tag="VFG22_13040"
     CDS             2634..>3060
                     /locus_tag="VFG22_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417214.1"
                     /translation="MIGALQIACARHLLRLAGVALVLILSSCTNVGAKAIRNTRFNYN
                     SAVVDTRNEQLLTNLVRLRYRDTPYFIEVSSIATQYVLGLGGSGSVGGIGDNTAGGVG
                     GSVSYEERPTITYVPLQGETFSRRLLAPVPMEVIVLLSNS"
BASE COUNT          620 a          952 c          966 g          522 t
ORIGIN      
        1 tatcagccgc ggagggtccc gcaaccgcgg cgaacgctca gcaggacgaa ggaaccccgc
       61 gcgacttggc cgcggagatc gaggccgctc taggaatccc tagcgactgc gtgcaggact
      121 ttcaggcagc tgaacccagg acgattcgag ttcaagtgag cgctaccgtg cgcccgtccg
      181 gagtcatcat cagcccttcg gcatccggtg cgggtcttag cccgagggaa cggcagtgca
      241 ttgcggcaag agtcggcgct atttcgttga gaccgttggg cgaggacgtc tcgcgccgag
      301 tctccacgga aatcgcgatc gagtactcac ctcctgcgag ccccagtccg agcctgggag
      361 tgcctgagcc ccaactgaga aacgtaaggg acccattgcc ccaggggccg gacatcgctc
      421 catccgggag gccgatcgga gaccgggaag gcaggccaat tccggagccg aacgccaggc
      481 ccatccagga tcggagctcc cgcgacacca gaagccgtaa gcctagagcg atcgacggcc
      541 acgagatcga cgacggcgcc gaagagtgga ggtagagatc atcctgggac ccgggcagcc
      601 gtggggtaac tcatggtgac gagtgcgacc aaatcgaggt cggcgggctc ttccaaaagg
      661 gtgtccttgg gcgggttcag tagaacgctg ccgctcgagc gggtccggac tccgagtgcg
      721 gtctgcccgc atgccgccgc cgagcgttct aggtccgcga acgtgagtcg acccgaatcc
      781 gcgtagtctg tcaccgggtg gaacgtgatc tccgcgccgc cggccgtgaa caactcgtcg
      841 aacaccgact gaagctcggg gcggagcgcg acctgcgtca ggaggtggct caccaccaga
      901 gggctgacga tcagctcgcc gcgagatcgg gtcaccaagc tcgcgttgtc gggatcgagc
      961 aattccagaa tgacggtagc ctccgtgctc cgttcgcgca ggcgttcatc gacgatgagc
     1021 gatccgaaga gcgtgcgcgc gtcggcctct tcggcggact ccatccaatc actgcccatg
     1081 aatacgatgc tgtccgccag ctcgggaccg agcgcctcga tgtcatctaa atccgtgaaa
     1141 tcgacttcta gctgcgtgat cgctagggac gatgcgtcga aagggcgatg gctgagcaac
     1201 tgctcgcggc gggcgatcgg caccttcgag ccgatgcgaa ccacgtagcg ctcatcgccg
     1261 taggttccga actcttcgag cagggcgggc acgcggtggt tccatccgag cacgagtatg
     1321 tgcctggtgt cgcgtctctc cgtattggtg cgtgtgtccg aagtctctcg tggaagccga
     1381 gcgagactgg gtgagtccga cggtttcgag tccacgtagg aatgagccaa gacgacgagc
     1441 ttgtcccctt cttccaggcg agtgccaggc cctgcgttga gtaagggcgc aaaggcgccc
     1501 cccgacggtc gaaccaagcc catgagcacc gcatttggaa agtaacggtg aaggtcttcg
     1561 atttcgagcc caagcaactc gtccgcctca cggatgtaga tctcgttccc ttcgcgatgc
     1621 gtgagcagct cgtcgtaaac cctagacagt cctgggtgcc ggatattctg tgcgagcaag
     1681 cgacttatga ctccgtcgct cgagatgact tcgatgtcgc ggcggtacgc cttgcgcgca
     1741 agatgaacct tccgcgcttc gaaaacctcc gcaacgactc ggggcggtcg ctcctcgccg
     1801 cacgacaagg cgtcgagcga aagcagggtc ttgatcgtct gagtgtcgct ttgcagggac
     1861 ttgtcgccga agtctgcccc tggaatcagg attgcggctg cgtgcgcggc gtccactcgc
     1921 gcgaggtgtt cgggtcgaag ggcggagcca gagcgaatcg tgactcgacg ctcatcccac
     1981 gcaggcccca cggcatcgcg gagattctgg acgaaggcag ctgtgacggt ctctgccaac
     2041 aagacgatgt gcaggccggg cccaccacga cggtccaaga accgacgtgc gcgaccctga
     2101 gagagcagca actcgcgaat gatggcgtcg gtgcgattgg tccatcccag gatgagaatg
     2161 tggccgtcgc gtgcgaccgg cgtcagcccg ctttcgagct tcgccattcg cgcatccagc
     2221 cattgcgtca tgatcgcgac cagcgcgccc aggaaaacca cgtacccaag caccgtgaga
     2281 acggtcgaca aagtcctatt gacgagaccg gtgtcgtcgc cgaggtagcc cgggtcgctg
     2341 aggcgcagga atgaccacca gaccgcttcc gggagttcac cgaatcctcg acccaaaacg
     2401 agcgcgagga caccaccggt caacgagatg agggcaacga gcactgctgc gagcaagaca
     2461 cggtggaagg caccgcgaac gagccagcgc tcgatggcga atctcgcgcg gtcgaaaggg
     2521 cgaaacggca tcgaatcctt aatatcctca gaaggccgcg cccccaaaaa agcaccgagc
     2581 cgttccggga cggcccggac aaacgcggca aatgggctat ccttcgggac caaatgattg
     2641 gagcattgca aattgcctgc gcgcgacacc ttcttcgcct cgcgggcgtc gcgctcgtgc
     2701 taattctatc gagttgcacg aacgttggcg ccaaggcgat ccgcaacacg cgcttcaact
     2761 acaattcagc cgtcgtcgac acgcgaaacg agcagttgct caccaacctc gtccgactgc
     2821 gctatcgcga cacgccctat ttcatcgaag tctcgagcat cgccacccag tatgttctcg
     2881 gacttggagg ctcgggcagc gtaggtggga tcggcgacaa cacggcaggc ggggtaggcg
     2941 gttcggtttc ctacgaggag cggccgacca tcacctacgt ccctttgcaa ggcgagacct
     3001 tctcgcggcg cttgcttgca ccagtgccca tggaggtgat cgtgcttctc agcaactccg
//
LOCUS       DASRZF010000299         6587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3661936, whole genome shotgun sequence.
ACCESSION   DASRZF010000299 DASRZF010000000
VERSION     DASRZF010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 6587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6587
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3661936"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VFG22_13045"
     CDS             <1..234
                     /locus_tag="VFG22_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417215.1"
                     /translation="DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSAGASAGVSAS
                     ASASASVSVSVSASASVSVSVSVSVSVPIARVY"
     gene            complement(228..1961)
                     /locus_tag="VFG22_13050"
     CDS             complement(228..1961)
                     /locus_tag="VFG22_13050"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET6417216.1"
                     /translation="MGIVRKTSRSKKTEDPLLGKVVAGRYRLESLLGEGGMGVVYRAR
                     HVLIDRVVALKLIRPDLRGETHLRAWMVREARAANRVDHAHIVDIHDVGETEDGELYL
                     VMEYLIGDSLSSQIAKGPMPIARAVDILEQMTAALARAHDLGVVHRDLKSDNIMVTAR
                     GGRKDFVKILDFGLAALTRDPRLAPKGAVFGTPEYMSPEQARGEDAVSASDLYALGIL
                     FFEMLTGRLPFRSGDRDELLEMQRKAKAPSPRKYRPDIPEHAERIILTLLEKNPEDRF
                     RDGHHLKEELKEMQRTLPSREWDSPTDLAPAAPPPPPPAQTPGVVEWSRTAANFLRAV
                     ARAYPDGNAPTEVQGASDQIWELASRASRLEGELESHSKKLETIEKRGRALRAEIGRK
                     VEDLAGEESRALRDASSERDMLSQLILKRKRAQREHAAAQAEVQASGTNIERVRRGYE
                     TLGATRAQVEVLEGVARDYERRARSKEDVAAERRKQIEELRAQLKRYSEALENDLQTG
                     RERIADRVREALKYEKAFGAASSLLLDHLRDKTECRELLEDIDKARKGRDPDDKPSEL
                     QELMEKHVAAQ"
     gene            2110..4110
                     /locus_tag="VFG22_13055"
     CDS             2110..4110
                     /locus_tag="VFG22_13055"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6417217.1"
                     /translation="MSEERPLLGPYHIERRLAAGGMAEVFVARREGLHGFQKRVALKR
                     ILPQFSSDPDFVWMFIDEARLAALLEHPNIVQVFDFGTMGDDLVLVMELVDGSNVSRL
                     LRAAPPDEPIPWDVALHIAYQTAQALDYAHHAVDENGESMEFVHRDVSPANILLSRSG
                     HVKLTDFGIATVRSRAPTTEDGQVRGKLGYMSPEQVLGKELSGKSDVFTLSTVMTEML
                     IGEPLFQGESELNVLLQIRDADLRVLARCERRIPQDIRALVSRGLQRDPAERPTAGAF
                     AEACAEVIRRRGMGHGPDRVARLLSQLNLIDGDAPEIHLPDANVPSLVDSDLMHHAGQ
                     ELIGPQAVTSPTIYRVQLPDGSVMGPMSYPRLIQLLTTGVIDSRSSKISKADGTYLDP
                     SRLPELTRFVTSPGLQWKLEELAQAEKQGQLRAATLLPVFYDIISRQQTGVLHLWQRN
                     RRKKIYFVEGRPEFVASTDKSELLGEYLVATGQCLRMEIEMALALLPKYGGRLGDALV
                     GLGILRPVELFRAIGDQVRGRLMEAFRWRRGEWAFVRGARSHEETFPTGQDPYELLRD
                     AAMEAHLEEIESVLEPLRERVVERCEDGPPLTVFRLAPVWVGVLDSVLGDSTLGGVLA
                     RESASGADLEPVYRALYLGLACGLVRTKVSPSEMPFREVYSA"
     gene            4119..4964
                     /locus_tag="VFG22_13060"
     CDS             4119..4964
                     /locus_tag="VFG22_13060"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HET6417218.1"
                     /translation="MSFVELKHPESTPSPVLVEVPHSGLQVPDEVESEIDATPLAVLR
                     DSDIYVDQLYERAPRNGATLLVSRVSRYVVDLNRGPDDVDSAAVPRHPRARHIPERGV
                     VWRARTDGTPLLRAPLSVEQFSRRLSLYYEPYHRTLREVAARIRNEHGRVVILAAHSM
                     PSTGRRTLGGRAIRRADIVPGTHGRSTADGRIIDLIDNHFRGAGLSVKHDDPYRGGWT
                     TRSYGAPKLGQHAVQIELNRALYVDESSSEIKKSDFAQLQTVLDQLVGKLGKMLTDSA
                     QFATG"
     gene            complement(4946..5203)
                     /locus_tag="VFG22_13065"
     CDS             complement(4946..5203)
                     /locus_tag="VFG22_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417219.1"
                     /translation="MEVVLRKVGRKPGRAVVGRLLRPPRKGSVVVIEFADGLHEYVTT
                     PVKRVLQLCGQDTYYLQTANSRYRLEVRSSESGVVDQPVAN"
     gene            5433..6242
                     /locus_tag="VFG22_13070"
     CDS             5433..6242
                     /locus_tag="VFG22_13070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6417220.1"
                     /translation="MATSVTATALDRYRASLKRVEILDAETERELAQSWAEGNEAAGA
                     RLIESSLPFVIAIAREYRRWGVPLEDLVQQGNLGLLKAAAKFDPEKSCRLITYAAYWI
                     RAEIRDYVVRSYRIVRLGTTRTERRAMRAFRRMSIDSPQALAEESGMPLTRAKQLWPL
                     LSRGDLSLDASVTDAPTAMERMSYKQPTPEEAVARSEAVHHVRAALESAMRSLTDREQ
                     RIVEARILSDEPCTLEALGREMGVSKERVRQLEVRARQKLRDRLEHLRPAA"
     gene            6333..>6587
                     /locus_tag="VFG22_13075"
     CDS             6333..>6587
                     /locus_tag="VFG22_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417221.1"
                     /translation="MALLTSLCLIGGVGCKRDLGDCNLDGETEDGRPIDGPAAFDIAY
                     RLTDGLPMYEGQALVQSTCGDGAFCHSPAATGTERIGVPAG"
BASE COUNT         1164 a         2055 c         2091 g         1277 t
ORIGIN      
        1 gacagcgaca gcgacagcga cagcgacagc gacagcgaca gcgacagcga cagcgacagc
       61 gacagcgaca gcgacagcga cagcgacagc gacagcgaca gcgccggtgc cagcgccggc
      121 gtcagcgcca gtgccagcgc cagcgccagc gtcagtgtca gcgtcagtgc cagcgccagt
      181 gtcagtgtca gtgtcagtgt cagtgtcagc gtcccgatcg cgcgcgtcta ctgagctgcg
      241 acgtgctttt ccatgagctc ttggagctcg gaaggcttgt cgtcggggtc tctgcccttg
      301 cgggccttgt cgatgtcttc gagtagctcg cggcactccg ttttgtcccg gaggtggtcg
      361 aggagcaggc tcgaggcggc gccgaaggcc ttttcgtact tgagcgcctc acgcacgcgg
      421 tcggcgatgc gttctcgtcc ggtctgcaaa tcgttctcga gcgcttcgct gtatcgcttc
      481 agctgagccc gaagctcttc gatctgctta cggcgttccg ctgcgacgtc ttccttcgat
      541 cgggcgcgtc gctcgtagtc acgggctacg ccttcgagaa cttccacctg agcgcgggtc
      601 gcgccgaggg tctcgtagcc acgacgtacg cgctcgatgt tcgtcccgga ggcttggacc
      661 tcggcttggg ccgccgcatg ctcgcgctga gcgcgctttc gcttgaggat caactggctc
      721 agcatgtcgc gctcggacga ggcgtcacgc agcgcgcgcg actcttcacc ggcgaggtct
      781 tcgaccttac gaccgatttc agcgcgaagc gcgcgacccc gcttttcgat cgtttcgagc
      841 ttcttcgaat ggctttcgag ctctccttcg agcctcgatg ccctagacgc cagctcccag
      901 atctggtccg acgcgccttg tacttcggtg ggcgcgttgc cgtctgggta cgcccgggcc
      961 acggcgcgaa ggaagttggc cgcggttcgg ctccattcga ccacgcccgg cgtctgtgca
     1021 ggcggcggag gcggtggagc agcgggagcc agatccgtcg gcgagtccca ttcgcgcgag
     1081 ggaagggtgc gctgcatctc cttcagctct tctttgagat ggtggccgtc gcggaatcgg
     1141 tcttccgggt tcttctcgag aagcgtgagg atgattcgct ccgcgtgctc agggatatcc
     1201 ggccggtact tgcgagggct cggcgccttt gccttgcgct gcatttcgag cagctcgtca
     1261 cggtctcccg aacggaaagg cagtcgaccc gtgagcattt cgaaaaagag aatacctaac
     1321 gcgtagagat cgctggccga gaccgcatct tcgccgcgag cctgctccgg cgacatgtat
     1381 tccggcgttc caaacaccgc tcccttgggt gcgagcctcg ggtcacgcgt caacgctgcg
     1441 aggccgaaat ccaggatctt cacgaagtct tttcggccgc cgcgtgcggt gaccatgatg
     1501 ttgtcgctct tgaggtcgcg gtgcacgacg ccgagatcgt gggcacgcgc aagtgccgcg
     1561 gtcatctgtt cgagaatgtc caccgcgcgc gcgatcggca ttgggccttt cgcaatctgc
     1621 gacgaaagcg aatccccgat cagatactcc atcaccagat agagctcgcc atcttcggtc
     1681 tcgcccacat cgtgaatgtc gacgatgtgc gcgtggtcga cgcggttggc agcacgcgcc
     1741 tcgcggacca tccaggcgcg cagatgcgtt tcgcccctca ggtccggccg aatcagcttg
     1801 agtgcaacaa cacggtcgat gagcacgtga cgagctcgat agaccacgcc catcccgcct
     1861 tcgccgagca gtgactccag tcgatatcga ccggccacca cctttccgag aagcggatcc
     1921 tcagtttttt tagagcgcga agtcttcctg acaatcccca tggttccccc gtacccagtg
     1981 gacattggta tgataagcag gaacggagcc gagtcccaag tgttccgctc tggctgaaac
     2041 ggggactaag ctgacaccgg gggcccaaga cgcgtatgat gagggcgtcg gtcacttacg
     2101 gagggatgtg tgagcgaaga acggccgcta ctcgggccgt accacatcga gcgtcgcttg
     2161 gcggcggggg gaatggccga ggttttcgtc gcgcgccgtg aaggtcttca cggctttcaa
     2221 aagcgcgtcg cgctcaagcg aattctcccg cagttctcta gtgatccgga cttcgtgtgg
     2281 atgttcatcg acgaggcgcg tctcgccgct ctgctcgaac accccaacat tgttcaggtg
     2341 ttcgactttg gcacgatggg ggacgacctc gtgctggtca tggagctggt cgatggctcg
     2401 aacgtgagcc gattgctgcg cgccgcgccg ccggacgagc cgatcccctg ggatgtggcc
     2461 ttacacatcg cctaccaaac ggctcaggcg ctagattatg cgcatcatgc cgtcgatgag
     2521 aacggcgaat ccatggagtt cgtccatcgg gacgtcagtc cagcgaacat cttgctcagt
     2581 cgctctggtc acgtgaagct caccgatttc ggtatcgcaa cggtgagaag tcgcgcgccg
     2641 accaccgaag acggccaagt tcgcggcaag ctcgggtaca tgtctcctga acaggtgctc
     2701 gggaaggagc tcagcggcaa gagcgatgtt tttacgctct cgacggtgat gaccgaaatg
     2761 ttgatcggcg agccactttt ccagggggag agcgagctca acgtgttgct ccagatccgc
     2821 gacgcggacc ttcgggtgct cgcgcgctgc gaacggcgca ttccgcagga catccgcgcc
     2881 ctggtctcgc gaggccttca gcgtgatcct gcggaacggc cgaccgccgg agcgtttgcg
     2941 gaagcctgtg cggaggtcat ccgccgccgc gggatggggc acggacccga ccgcgtcgcg
     3001 cgactccttt cacagctcaa tttgatcgac ggggatgcgc ccgagattca ccttccggac
     3061 gccaacgtgc cctcgctggt ggacagcgat ctcatgcatc acgccggcca agagctgatc
     3121 ggaccgcagg cggtgacctc gccgaccatt taccgcgttc aacttcccga cggaagcgtg
     3181 atggggccga tgagctatcc gcgcctgatc cagctgctga cgaccggcgt gatcgacagt
     3241 cgcagcagca agatctccaa ggcggacggt acgtacctcg atccgtcgcg ccttcccgag
     3301 ctcacgcggt tcgtcacatc gcctggactt cagtggaagc tcgaagagct cgcacaggcc
     3361 gagaagcagg gacagcttcg tgctgcaacg ctccttccgg tattttatga catcatctct
     3421 cggcagcaga ccggcgtgct ccatctctgg caacgcaatc ggcgcaagaa gatctacttc
     3481 gtcgagggac ggccggagtt cgttgcgtcc acggacaagt cggagcttct cggcgaatac
     3541 ttggtggcca cggggcaatg cttgcgtatg gagatcgaga tggcgcttgc tctcctgccc
     3601 aagtatggag gacgactcgg cgatgctctg gtcggtctcg gaatcctgcg gccagtcgag
     3661 ctcttccggg ccatcgggga ccaagttcgc ggccggctga tggaagcatt tcgctggcgg
     3721 cggggcgagt gggccttcgt ccgcggcgct cggagtcacg aagagacctt ccctactggg
     3781 caggatccat acgagcttct gcgcgatgct gcgatggagg cgcacctaga ggagatcgag
     3841 tccgtcctcg agccccttcg tgaacgggtc gtcgagcgtt gcgaagatgg gccgccgctg
     3901 acggtgttcc gccttgctcc ggtctgggtg ggcgtactcg attccgtttt gggggattct
     3961 acgctcggcg gggtgctcgc acgcgagagc gcgtcaggtg ccgatctaga gcctgtatac
     4021 cgggccctct atctggggct tgcgtgtggg ctggttcgta ccaaggtgag cccttcggag
     4081 atgccctttc gcgaagtcta tagcgcctga gcccatctgt gtctttcgtc gagctaaaac
     4141 acccagaatc gacaccgagt cccgtgctcg tcgaggtccc ccactcgggc ttgcaggttc
     4201 cggacgaagt cgaatccgag atcgacgcaa cgcccttggc ggtcttgcgg gactccgata
     4261 tttatgtcga tcagctttac gaacgtgcac cgcggaatgg tgcgacactc ttggtgtcac
     4321 gcgtctctcg gtacgtggtc gacctcaacc gcggcccaga cgacgtcgac tctgctgcgg
     4381 tgccgaggca tccgcgggcg cggcacattc cggagcgtgg cgtagtctgg cgtgctcgaa
     4441 ccgatgggac cccgctcttg cgtgcgccgc tctcggtcga acagttctca cggaggcttt
     4501 cgctctacta cgaaccatac caccggacgc tgcgcgaagt agccgcacgg attcgcaacg
     4561 agcatggccg cgtcgtcatc ttggccgcgc attcgatgcc ctcgacgggt cgccgaacgc
     4621 tgggtgggcg agcgattcgc cgcgccgaca tcgtgcctgg cacccatggc cgttccacgg
     4681 cggatggtcg catcatcgat ctcatcgaca accacttccg cggcgctggg cttagcgtga
     4741 agcacgacga tccctacagg ggaggctgga cgactcggag ctacggtgct cccaagctcg
     4801 gacaacacgc cgtgcagatc gagctcaacc gtgcactcta cgtcgatgag tccagcagcg
     4861 agatcaagaa gtcagatttc gcgcagttac aaaccgtgct cgaccagctt gtgggcaagc
     4921 tcgggaagat gctgacagac tcagctcagt tcgcgacggg ctgatccacc acgcccgact
     4981 cgctcgaacg aacttcgagg cggtaacggc tgttggcagt ctgaaggtag taagtgtcct
     5041 gcccgcagag ctgaagcacg cgcttcaccg gggtcgtgac atattcgtgt agcccatcgg
     5101 caaactcgat gacgacgacg gagcccttgc gtggcggccg tagcaagcgt cccacgacag
     5161 ctcgacccgg cttacggcca actttgcgca gaacgacttc cacgccacga aactagcata
     5221 actgtctgcc ccagcaaagc tgacacggaa aaatccagct attttgcgcc tccgtggatt
     5281 ttgcgtcggc cgcgcggaac ttgcgcgttg cgccgcggcg agaaagctgc tccgtgtggc
     5341 aaaatgccgc ggtcccccac tgactctgtc cattttccag cgttttttgt gggtcgccgg
     5401 ccgggcacga atagtgcatc gggggttcgg ccatggcgac aagcgttacc gcaacggctc
     5461 tggaccgata ccgcgcgtca ctcaagcggg tcgagatcct agacgccgaa accgagcgcg
     5521 agctcgcgca aagctgggcg gaaggcaatg aagctgccgg cgcgcgtttg atcgagtcga
     5581 gcctgccatt cgtcatcgcc attgcgcgcg agtaccgccg atggggcgta ccgctcgagg
     5641 atctggtcca gcagggcaac ttgggtcttc tgaaggcggc agcgaagttc gatcctgaaa
     5701 agagctgtcg actgatcacc tacgcggcct actggatccg cgcggagatc cgcgactacg
     5761 tcgtgcggtc ttaccgaatc gtgcgtctgg gaacgacgcg caccgagcgc cgcgcgatgc
     5821 gcgcgtttcg tcgaatgtcg atcgattcgc cgcaggccct tgcagaagag agcggcatgc
     5881 cgctcacacg tgcgaagcag ctctggccgc tcctgtcacg tggagatcta tccctcgacg
     5941 catcggtgac agatgcgccc accgcgatgg agcgaatgag ttacaagcag cccacgccgg
     6001 aggaggcagt cgcgcgttcc gaggccgtgc accatgtccg tgctgccctg gagtccgcga
     6061 tgaggagctt gactgaccga gagcaacgca tcgtggaagc ccggatcctg tccgacgagc
     6121 catgcacgct cgaggcgctc ggccgtgaaa tgggcgtgag caaggagcgc gtgcgtcagt
     6181 tggaggtgag ggcccgccag aagctccgcg atcggctgga gcatctgagg cccgcggcct
     6241 gagcttcggc cgcaactcgc ttgactctcc agggcatcag aagcaatttg ccggctcaga
     6301 tgctttctgg tcgcgttctt gcttgttttt cgatggctct cctcacgagc ctctgcctca
     6361 tcggcggagt cggttgcaag cgggacctcg gcgattgcaa cctcgatggc gagaccgagg
     6421 acgggcggcc tatcgacggt cctgccgcgt tcgacatcgc gtatcgcctc accgacggct
     6481 tgccgatgta cgaagggcaa gcgctcgtgc agtcgacctg cggcgacgga gcgttctgcc
     6541 attcgcccgc tgcgaccggt accgaaagga ttggcgttcc ggcaggt
//
LOCUS       DASRZF010000300         5119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2980351, whole genome shotgun sequence.
ACCESSION   DASRZF010000300 DASRZF010000000
VERSION     DASRZF010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 5119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5119
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2980351"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VFG22_13080"
     CDS             complement(<1..350)
                     /locus_tag="VFG22_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417222.1"
                     /translation="MNKSLLGLSLLLGSGLWMVGCGDDGGCPGGQVSCDGVCIDAIEP
                     TLAGVDGIQERVFEGSCAFSNCHGAEGLPQADLELSSATTSADNLIDVQSTEVDGLRV
                     APGNTGASYLVDKLL"
     gene            598..1932
                     /locus_tag="VFG22_13085"
     CDS             598..1932
                     /locus_tag="VFG22_13085"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET6417223.1"
                     /translation="MKGHWFWGSLFELIRDRSGFLIEGHAKYGDVFVARALTRDLMCV
                     RDPALVNAINVTYWADFYKPDYIKLMWKPFLGNGLVPNDGESWKRQHKLILPGFHKKR
                     VDAYASTMVAFTERMVDRWKEGERRDMRLELNALALEVVADTLFAIDIGKGDSKTIRD
                     ALADISKILVTDVDKMIPRPDWWPSAENRRKKRAIAKIEEIIRRVHQERLTHKQDRGD
                     LFSHMVFVEDEQGPMSDKQLRDEAMTLIFAGHETTAHALTWTWYLLAKNPDKVAKMRE
                     ELTRVLHGRNAEVEDLPELPYLEMVVKESLRLLPSVWAYARQAQRDLVIEGYEIKKGQ
                     TITISHIAMGRNLAYYDSPEEFRPERWTREFERSLPRGAYTPFAGGPRVCLGKQFAMM
                     EMRMILATLIQRLEPNLAEGFEPDFVTELSMHPGERGMQMDVRFYDAAPTSL"
     gene            2068..2571
                     /locus_tag="VFG22_13090"
     CDS             2068..2571
                     /locus_tag="VFG22_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417224.1"
                     /translation="MVRNLMMILGLSLAVAAFGCDSSSEGNGGSGGDAGTGGMAGTGG
                     MAGTGGMAGEGGTGGSVAPMDACTNVDDATIVCDPGFADDVRTCATAAIGNGAATATC
                     LVEDFGVSADCASCFGDETQCIFDNCLGSGCAATPEGDACLSCRAENCDAEAAACKGD
                     LETACAG"
     gene            2746..3978
                     /locus_tag="VFG22_13095"
     CDS             2746..3978
                     /locus_tag="VFG22_13095"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET6417225.1"
                     /translation="MQSLEPSEEQVAYDPIYDPFSPDLIADPYPFYRRLRDEHPVYYN
                     ERMDFYVLSRYDDIFHALRKPQLFSSAEGLTPAKDEISQLGLPPTFIMMDPPDHTRLR
                     RLITKAFTPDTVKELEPTIRQFVIDHVEAMKQKSADGEEVDLIEGIASPLPTMVVANI
                     LGVPPEDREKFDPWSDAITSASADFALDLKQAIGAVQGLVSYFGAMCQARRAKPTGDM
                     ISKLTQAEVDGETLSDWDILGFCFVMIAGGNDTTTNLIGNSMKFLHDYPDQHREMLGN
                     PSLIPNAIEELLRIESVVQGLSRTTTEDVELHGTRIPGGSKVHNIYASGNRDPRHWGD
                     DADELDIHRKIDRHLSFAQGPHFCIGAHVARLMVRTYFEEMGSRIGENFEIDWDRAER
                     RHSAFTRGFTKIPIYIKA"
     gene            3975..4283
                     /locus_tag="VFG22_13100"
     CDS             3975..4283
                     /locus_tag="VFG22_13100"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET6417226.1"
                     /translation="MSMIRVRFEPSGFEVDAQPGEAIMDITDANPAAEVPYSCRAATC
                     GTCRVEVVAGGDALSTPDEFELEVLEIFGDTPDRVRLCCQARIVGGAHGPVVLKVCDD
                     "
     gene            complement(4300..4797)
                     /locus_tag="VFG22_13105"
     CDS             complement(4300..4797)
                     /locus_tag="VFG22_13105"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HET6417227.1"
                     /translation="MRSDIVKIKPKPEGLDKPYVGKIIKTMSKVHVAIYLWTGGLLGS
                     TWRVGSAFPRGIPVLLLTTIGRKSGQPRIAPLLFIEDGGNVIIVGSQGGLPKDPLWYK
                     NLQANPDCEVQIKRRKMKMRAHTASPEERARLWPKLVDHYADFASYETWTDRVIPVVV
                     LEPVA"
     gene            complement(4805..>5119)
                     /locus_tag="VFG22_13110"
     CDS             complement(4805..>5119)
                     /locus_tag="VFG22_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417228.1"
                     /translation="ITVQPGPFRTDMVAGIERAFTQAENETPKFASVIRALKGLAAKQ
                     IDSAHDPDILAQAIETALTAKRPKPVYSVKPDRLRSSLERLPLRAVDRIYVTVMRQAS
                     KE"
BASE COUNT         1023 a         1586 c         1542 g          968 t
ORIGIN      
        1 agcagtttat cgaccaggta ggaggcgccc gtgttcccag gggcgaccct gagcccgtcg
       61 acttcggtgg actgcacgtc gatcaggttg tcagccgatg tcgtagccga gctgagctcg
      121 aggtccgctt gcggaaggcc ctcggctccg tggcaattgc tgaacgcgca gctgccctca
      181 aaaacgcgtt cctgaatgcc gtcgacgccc gcaagcgtcg gctcgatcgc gtcgatgcac
      241 accccgtcgc agctcacttg cccgcccggg cagcctccat cgtcaccgca acccaccatc
      301 cacaagcctg agccgagaag cagggacaac ccaagaagag atttgttcat tgaaggcata
      361 tagtttgcgc cacccctcta gtaaaggctt cagacgcgcc ttcgccttac gatttggctc
      421 ctcgccgcgt catcgcggag cggcgtacct gggtgcccct tgctcccgtc ggacccccca
      481 cttactctcg cgtcccatga gcttgatgtc gcgcacccga tggacccttc ttgaatgacc
      541 gccacgacca gatcgccggc ccgccagagc tcgattgcga cccgcgaggt tccgcgcatg
      601 aaagggcatt ggttctgggg aagcctcttc gagctcatcc gcgaccgctc cgggttcttg
      661 atcgagggcc acgcgaaata cggagacgtc ttcgtcgccc gcgcgctcac ccgcgatctg
      721 atgtgcgtcc gagacccagc gctcgtcaac gcgatcaacg tcacgtattg ggcagacttc
      781 tacaagcccg actacatcaa gctcatgtgg aaaccgttcc tcggcaacgg tctcgttccg
      841 aacgatggtg agtcctggaa gcgccaacac aagttgatcc tgcccgggtt ccacaagaag
      901 cgcgtcgacg cctacgcatc gacgatggtt gctttcaccg agcgcatggt cgatcgctgg
      961 aaggaaggcg agcgaaggga catgcgcctc gagctcaacg ccctggccct ggaggtcgtt
     1021 gccgacacgc ttttcgccat cgacatagga aagggcgact ccaagacgat ccgcgatgcg
     1081 ctcgccgaca tcagcaagat tcttgtcacg gacgtcgaca agatgattcc acgcccggac
     1141 tggtggccga gcgcggaaaa ccgcaggaag aagcgcgcaa tcgccaagat cgaagagatc
     1201 atccggcgcg tgcaccagga gcgcctgacc cacaagcaag accgcggcga cctgttctct
     1261 cacatggtgt tcgtcgagga cgagcagggc ccgatgtccg acaagcagct tcgcgacgaa
     1321 gcgatgactc tcattttcgc cggacatgaa acgactgctc atgcgctgac ttggacctgg
     1381 tacctgctcg caaagaaccc ggacaaagtg gcgaagatgc gcgaggagct cactcgggta
     1441 ctgcacggca ggaacgccga ggtcgaagat cttccagagc tcccctacct cgaaatggtc
     1501 gtgaaggagt cccttcggct cttgccctcc gtctgggcct acgcacgtca agcgcaacgc
     1561 gacttggtca tcgagggcta cgagatcaag aagggccaaa cgatcaccat cagccacatc
     1621 gcaatgggcc gtaacctcgc ttactacgac agcccggagg agttcagacc cgagcgctgg
     1681 acgcgcgagt tcgagcgaag tcttccgcgt ggggcgtaca cccctttcgc gggcggaccc
     1741 cgggtgtgcc tcgggaagca gttcgcgatg atggaaatgc gcatgatcct cgcgacgctg
     1801 attcagcgtc tcgagccaaa cctggccgaa ggcttcgagc ccgacttcgt gacagagctt
     1861 tcgatgcacc caggggagcg cggcatgcag atggacgtgc gattctacga cgccgccccg
     1921 acgtctctct gaaagcggaa ttacggtgtc gacccagcct cgattgaggg gaatcgcaca
     1981 gttttcgggc ctagagtcat cttgaacgct atgatcggca gcttagagaa ggcaggtctt
     2041 ctcgatacct gtttagggga ggtggatatg gttcgtaact tgatgatgat tcttggactc
     2101 agtcttgcgg tcgcagcctt cggttgtgac agcagcagcg aaggcaacgg tggttcgggc
     2161 ggggacgcag gcaccggcgg aatggccggc accggtggaa tggccggcac cggtggaatg
     2221 gccggcgaag gtggcaccgg cggttcggtg gcgccgatgg atgcttgcac caacgtcgac
     2281 gatgcgacga tcgtttgcga tcccggcttc gctgatgatg tccggacctg cgcgacggcg
     2341 gcgattggca atggcgcagc gaccgcgacc tgtctcgtgg aggactttgg cgttagtgca
     2401 gattgcgcga gctgcttcgg tgacgagact cagtgcatct tcgacaactg cctgggctca
     2461 ggctgtgccg cgacgcccga gggcgacgct tgtctctcat gtcgcgccga aaactgcgac
     2521 gcagaagcgg ctgcctgcaa gggcgatctc gaaaccgcat gcgccggcta aagctcgaca
     2581 gatagctcta aattgacagg atcgagccgg cgctggtcat tggccagcgc cggtttcttt
     2641 ttttgaattt cccgaacggc gaaacatggc agactggaac ccctcgcaga cgccgtcgtc
     2701 gtcgagcacg acaatcgcgc gcgaccgagc agaggagcgg gaaacatgca aagcctggaa
     2761 ccaagcgaag agcaagtcgc gtacgatccc atctacgatc cgttcagtcc ggacctgatc
     2821 gccgatccct atcctttcta tcggcgcctc agggacgaac atcccgtcta ttacaacgag
     2881 cgcatggatt tctacgtgtt gtcccgctac gacgatatct tccacgccct tcggaagccg
     2941 cagctctttt catctgctga ggggctcacg cccgccaagg acgagatcag ccagttaggg
     3001 cttccgccga cgttcatcat gatggacccg cccgatcaca cgcggcttcg gcgtctgatc
     3061 accaaggctt tcacgcccga cacggtcaag gagctcgagc cgacgattcg gcagttcgtc
     3121 atcgatcacg tggaggcgat gaagcagaag tcggccgatg gggaggaggt cgatctcatc
     3181 gagggaattg cctctccctt gccgaccatg gtcgtcgcga acattctggg agtgcccccc
     3241 gaagaccgcg agaagttcga cccttggtcg gacgccatca cctcggcttc ggcggacttt
     3301 gccttggatc tcaagcaggc aatcggcgcc gttcagggct tggtcagcta tttcggagcc
     3361 atgtgccagg cgcgacgcgc gaagccgacg ggcgacatga tcagcaagct cacgcaagcg
     3421 gaagtcgatg gagagacgct gagcgactgg gacatcctcg gcttttgctt cgtgatgatc
     3481 gcgggcggca acgacaccac caccaatctc atcggaaact ccatgaagtt cctccacgac
     3541 tacccggatc aacatcggga gatgctcggg aacccgtcgc tcattccgaa cgcgatcgag
     3601 gagctcctca gaatcgagtc ggtcgttcag ggcttgtcgc gaaccacgac cgaggacgtc
     3661 gagctccacg gaacgagaat tcccggaggg agcaaggttc acaacatcta cgcatccggc
     3721 aatcgcgacc cccggcactg gggcgatgac gccgacgagc tcgacattca ccgaaaaatc
     3781 gatcgccacc tctcatttgc ccaaggtccg cacttctgca tcggcgcaca cgtggcgcgg
     3841 ctcatggtgc gcacctattt cgaagagatg ggttcacgca ttggcgagaa tttcgagatc
     3901 gactgggaca gggccgagcg gcgtcactcc gccttcacgc gcggctttac gaagatcccg
     3961 atttacatca aagcatgagc atgattcgag tgcggttcga gccctcgggt ttcgaggtcg
     4021 acgcccagcc cggcgaggcc atcatggaca tcaccgacgc caatcctgcg gcggaggttc
     4081 cctactcttg ccgcgcggcg acctgtggca cctgcagagt cgaggtcgtt gcgggcggcg
     4141 acgcgctctc cacgccggac gaattcgagc tcgaagtcct ggagatcttc ggggacacgc
     4201 cagatcgggt ccgcctctgt tgccaagcca gaatcgttgg cggcgcccac ggcccagtcg
     4261 tcctgaaggt ttgcgacgat tgaacgccga gagaggacct caggcgaccg gctcgagaac
     4321 gaccaccggg atcacgcgat cggtccaggt ctcgtaactt gcgaagtcgg cgtaatgatc
     4381 gacgagcttc ggccagagac gggcgcgttc ctcgggcgaa gcggtatggg ctctcatctt
     4441 catcttgcgc cgcttgatct gaacttcgca gtccgggttc gcctggaggt tcttgtacca
     4501 gagcgggtcc ttcggaagac cgccctgtga gcccactatg atgacgttcc ccccatcttc
     4561 gatgaagagg agcggtgcga ttctcggctg gccgctcttg cgcccgatcg tcgtcagcag
     4621 caagacgggg atgccccgag gaaacgcgct tccaacacgc caagtcgagc cgagcaatcc
     4681 gcccgtccag aggtagatcg ccacgtgcac cttcgacatg gtcttgatga tcttccctac
     4741 gtagggcttg tcgaggccct cgggcttggg tttgatcttc acgatatcgc ttctcatggt
     4801 gtttctattc cttgctggcc tgtcgcatca cggtcacgta gatgcgatcg accgcgcgca
     4861 ggggaagcct ctcgagagac gatctcaaac ggtccggctt caccgagtaa accggcttcg
     4921 ggcgcttcgc cgtgagcgcg gtttcgatcg cctgagccaa gatgtctgga tcatgcgcgg
     4981 agtcgatctg cttggcggcc aagcccttga gcgcgcgaat caccgaagca aacttcgggg
     5041 tctcgttctc ggcctgggtg aacgctcgct cgattcctgc caccatatcg gtgcgaaacg
     5101 gtcccggctg aaccgtgat
//
LOCUS       DASRZF010000301         5522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_917387, whole genome shotgun sequence.
ACCESSION   DASRZF010000301 DASRZF010000000
VERSION     DASRZF010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 5522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5522
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917387"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="VFG22_13115"
     CDS             complement(<1..686)
                     /locus_tag="VFG22_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HET6417229.1"
                     /translation="MLKATVGLDVGSTTVKAVVVDPETKDIVWSDYKRHHTKQAECVL
                     DFMVRIEQVLAGLGCDQVRVFATGSGAKPLEAPLGAKFVQEVNSVTLAVETLHPDAGS
                     VVELGGQDAKIIIFKENKQTGDKSAIASMNDKCASGTGATIDKCMIKVGMPSEETLNL
                     HFDDAHLHHVAAKCGVFAETDIVNLVKTGIPSDEIMCSLADAIVMQNLSVLTRGNTLR
                     HKVLLLGGPNT"
     gene            836..2023
                     /locus_tag="VFG22_13120"
     CDS             836..2023
                     /locus_tag="VFG22_13120"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HET6417230.1"
                     /translation="MSKVLIIGAGGVGGVVTHKCADDRDTFSEVVLASRTLSRCENIQ
                     RQVRELRGRQIEIAQVDADDVAQTVALIKRVQPDLLINVALPYQDLPLMDACLEAGVD
                     YLDTANYEPPDEAKFEYKWQWAYRERFQSAGIMGLLGSGFDPGVTNVFCAYAQKNLFD
                     EIDYIDILDCNGGDHGRPFATNFNPEINIREITQRGRYWENGEWVEIDPMSQSRMFDF
                     PAVGERKAYLLYHEELESLVKHIDGLKRIRFWMTFGDEYITHLRVLQNVGMTGIEPVE
                     FQGREIVPIQFLKALLPDPSTLGENYTGKTSIGCLIEGRKDGKPRKVFIYNVCDHEET
                     WKEVRAQAVSYTTGVPTMVGAKMMLTGKWRGKGVFNVEQFEPEPFLEELAVRGLPWHV
                     KEM"
     gene            2023..3174
                     /gene="nspC"
                     /locus_tag="VFG22_13125"
     CDS             2023..3174
                     /gene="nspC"
                     /locus_tag="VFG22_13125"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:TIGR01047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HET6417231.1"
                     /translation="MLPCAQLDLSRVDSPAFVIDLAALEGNLQLLARVQRAANCRVLL
                     ALKGFAAWSTFALVGRYLEGVAASGPDEARLGREELGKQVHTYCPAFDEASLLETIRY
                     SDHVTFNSPSQIKRFRALVDEHGSDTSFGLRINPEHSEVEVAIYDPCARGSRLGTTKS
                     ELIGADLRGIEGLHFHTLCELGADALQRTLAVIEDKFSPWLREAKWVNFGGGHHITRP
                     GYDVDLLVDLARAFKEEHGVEVFLEPGEAIGLNTGVLIASVLDIVENDGQIAILDISA
                     TAHMPDVLEMPYRPQVRDAAEPGKLPHTYRLGGLSCLAGDVIGDYSFHRPLEIGDKLV
                     FEDMAHYTMVKTTTFNGVRLPSIALYDPRDESYRVQRRFEYEDYRNRLS"
     gene            3212..3724
                     /locus_tag="VFG22_13130"
     CDS             3212..3724
                     /locus_tag="VFG22_13130"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET6417232.1"
                     /translation="MGAFSDFTAYAKETGSRLSDQPLLLEKTRHLDVSQSELFHYVTD
                     FERSSEWIAGALKGWTDNSNAETPGGLGSVRVIKSAAGEPVREVVKAYDAPRMFAYSA
                     GDAAFLGLCFDHLGVMTLEPHPDGGTVLCWLAYGRLASNPVKAWAGKKLFQVALGRGM
                     KNLERKFPPR"
     gene            3729..>5522
                     /locus_tag="VFG22_13135"
     CDS             3729..>5522
                     /locus_tag="VFG22_13135"
                     /inference="COORDINATES: protein motif:HMM:NF016169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HET6417233.1"
                     /translation="MELIRSNRTEKLAAALASRVCEKPLGPFAREVIVVQSRGMARWL
                     TLALAEKLGSWSNPAFPFPRAVIEQVLTRIEEGRAEHAKAYEPAQLKWTIAGLLRESA
                     PVELEAYLGVPSNPDRVLRLASSASVVFDRYVLYRPTLLRQWSNGAGGDWQAELWRRV
                     VQRLGPHDLASRIRDALETLRSTKVVAELPFERLHLFSLETLPPLFLELFSALSQSVE
                     TTFYVLEPSSEYVSDVDPTLGDLISPDAAPSDGHPLLSSLGRLARDFQQVLLSADRAV
                     QYEADLFEAPKRDNLLGALQADILEFRSAPKNSERRIIDPADDSISIHACTGPMREVQ
                     ALHEWVQSALESDRSLQPEDVVVMTPDLDTYAPAFRAVFGQDDLHRIPYEVHDRSTRD
                     DAAFYDDFQTILELLDSRFSVLDFVRILDAGALRATFLFTQEERARLAELLAASGIRW
                     GIDAAHREALEFPADPLHTWRAGLGRLFLGFASTPDDTSPFEGLLPRGAPSLSDAELL
                     ARLSRLSEVLFDFHRRSREPLSIEDWARELEHFSALLFAEDDESSSAMRTLREAIADL
                     RDVATRTGYDCPIALKTLRRELGAILQEKAPA"
BASE COUNT         1086 a         1687 c         1651 g         1098 t
ORIGIN      
        1 gtgttgggac cacccagcag caaaaccttg tgccgaagcg tattgccgcg ggtgaggacc
       61 gatagattct gcatgacgat cgcgtcggcc agcgagcaca tgatctcgtc ggaaggaatg
      121 ccggttttga cgaggttgac gatgtcggtt tcggcgaaga ctccgcactt cgcggcgacg
      181 tggtgaaggt gtgcgtcgtc gaagtggagg ttcaaggtct cttcggaagg catgccgact
      241 ttgatcatgc acttgtcgat cgtggcgccc gtaccggacg cgcacttgtc gttcatcgat
      301 gcgatcgccg acttgtctcc ggtctgcttg ttctctttga agatgatgat cttggcgtcc
      361 tggccgccga gctcgaccac ggaacccgcg tcgggatgaa gggtctcgac ggcgagtgtc
      421 accgagttca cctcttgaac gaacttcgca ccgagcgggg cttcgagcgg tttcgctcca
      481 gacccagtgg caaacactct gacctggtcg caaccgagcc cagccaggac ttgctcgatg
      541 cgcaccatga agtcgaggac gcactccgcc tgtttggtgt ggtggcgctt gtaatcgctc
      601 cagacgatgt ctttggtctc cgggtcgacc accaccgctt tgacggtcgt ggaaccgacg
      661 tccaaaccca ctgtcgcttt cagcataaga tcggttcagc ccttccctcg caagctagac
      721 gaggtaaaca caccatctgg tgaaagtcac gcgagttggc ttgcaaatcg gggacgtcga
      781 cgtcacacct cacactgcag gcggacagct tgtatagccc aatttggagg actccatgag
      841 caaggtcttg atcattggcg ctggtggcgt aggcggtgtt gtcacccata aatgcgcaga
      901 cgatcgcgac acgttttccg aggtcgtact tgcaagccgc acgctttcgc gttgtgagaa
      961 catccagaga caagtgcgcg agctccgcgg ccgtcagatc gaaatcgccc aagtggatgc
     1021 ggacgacgtc gcacagacgg tcgccttgat caaacgagtg caaccggact tgctgatcaa
     1081 cgtcgccctc ccttaccaag acctgcccct catggatgcc tgcctcgagg cgggcgtcga
     1141 ctacctcgat accgccaact acgagccgcc ggacgaggcc aaattcgaat acaagtggca
     1201 gtgggcgtac cgggaacggt tccaatctgc tggaatcatg ggacttttgg gatcggggtt
     1261 cgaccctggg gtcaccaacg tgttttgcgc atatgcccag aagaatcttt tcgacgagat
     1321 cgactacatc gacatcctgg attgcaatgg tggcgaccat ggccgcccgt tcgccacgaa
     1381 cttcaaccca gagatcaaca ttcgcgagat cacccagcgc ggccgatatt gggagaacgg
     1441 cgaatgggtc gagatcgacc cgatgtcgca gtcgcgcatg tttgattttc cggcggtcgg
     1501 cgagcgcaaa gcctaccttc tgtaccacga agagctcgaa tccctggtta agcacatcga
     1561 tggtctcaag cgcattcgtt tctggatgac ctttggcgac gagtacatca cccaccttcg
     1621 tgtgctccag aacgtcggaa tgaccggcat cgagcccgtc gagttccagg gccgcgagat
     1681 cgtgccgatc cagtttttga aagccttact gcccgatcca tcgacgctgg gtgagaacta
     1741 cacgggcaag acgagcatcg gttgcctgat cgaaggccgc aaagatggga agccccgcaa
     1801 ggtgttcatc tacaacgtct gcgatcacga agagacctgg aaggaagttc gcgctcaggc
     1861 tgtctcctac acgaccgggg ttcccaccat ggttggagcc aagatgatgc tgacgggtaa
     1921 gtggcgcggc aaaggcgtgt tcaacgtcga gcagttcgag ccggagcctt tcctcgagga
     1981 gctcgcagtg cgtggccttc cttggcacgt aaaggagatg tagtgctgcc ttgcgcgcag
     2041 ctcgatctca gccgagtcga ttcacctgcg ttcgtgatcg atctcgcggc actcgaaggc
     2101 aacttgcagc tactggcccg ggtgcagcgt gcggccaatt gccgagtctt actcgcgctc
     2161 aagggctttg cagcctggtc tacgttcgcg ctggtgggca ggtacctcga gggggtggcg
     2221 gccagcggcc ccgacgaggc gcgactcggg cgggaggagc ttggcaaaca agttcacacc
     2281 tattgtcccg cgttcgacga agcttcgctt ctggagacga ttcgctactc cgaccatgtg
     2341 accttcaact cgccgtcaca gatcaaacgc ttccgcgctc tagtcgatga gcatggctcg
     2401 gacacgagct tcgggctgcg gatcaatccc gagcactcgg aagtcgaagt cgcaatctac
     2461 gatccctgtg cacggggatc taggctcgga acgaccaaga gcgagctgat cggtgccgat
     2521 cttcggggca tcgaaggcct ccacttccac accctttgtg agcttggagc cgatgcgctg
     2581 cagcggacgc ttgcggtcat cgaagataag ttttcgccct ggttgcgtga agcgaagtgg
     2641 gtgaacttcg gcggcggaca tcacatcacg cgccctgggt acgacgtgga cctcctggtg
     2701 gacctggcgc gtgcattcaa ggaagaacac ggagtcgaag tgtttctgga gccaggtgaa
     2761 gcgatcgggc tcaacacggg cgtgctcatc gcgtccgttc tcgatatcgt cgagaacgac
     2821 gggcaaattg cgattctcga catctcggca acagcgcaca tgcccgatgt tctcgagatg
     2881 ccctaccgcc cccaggtgcg ggacgcggcc gaacccggaa agcttcccca cacctatcga
     2941 cttggcggtc tgagctgcct cgccggcgac gtgatcggag actactcctt ccaccggccc
     3001 ctggagatcg gtgacaagct ggtattcgaa gacatggccc attacacgat ggtcaagacg
     3061 acgaccttca acggggtccg actgccgtcg atcgcgctct acgacccgcg tgacgagtcg
     3121 taccgggtac aacggcgctt cgagtacgaa gactaccgaa accgcctctc ttgatgcttg
     3181 aacttcgatc gaaagcgata agactgccgt gatgggcgct ttctcggatt tcacagcata
     3241 cgccaaggaa acgggctctc gactcagcga ccagccgctg ctcctcgaga agacgcgtca
     3301 cctcgacgtc tcgcagagcg agctcttcca ctatgtcacc gacttcgaac gctcgagcga
     3361 gtggatcgcc ggtgccttga agggctggac cgacaacagt aacgcagaga ctccaggcgg
     3421 acttggatcg gttcgcgtca tcaagagcgc cgcaggggag cccgtgcgcg aggtcgtaaa
     3481 agcatacgat gcaccgcgga tgttcgccta tagcgccggt gacgccgcat tcctcggact
     3541 ctgcttcgac catctaggtg tgatgactct cgagccgcat ccggacgggg gcaccgtact
     3601 ctgctggctc gcgtacgggc gcctggcaag caatccggtc aaggcctggg ccggcaagaa
     3661 gctgttccaa gtcgcgctcg gaagaggtat gaagaacctg gagcgaaagt ttccgcctcg
     3721 ctgagcgcat ggagctcatc cgcagcaatc gaaccgaaaa actagccgct gcgctcgcgt
     3781 cgcgagtttg cgagaagccc cttggtccat tcgcccgcga ggtcatcgtc gtacaaagcc
     3841 gcggcatggc gcgctggctc acgctcgcct tggctgagaa gctcggcagc tggagcaacc
     3901 cggcatttcc ttttccgcga gccgtcatcg agcaggtgct gacgcgcatc gaagagggcc
     3961 gcgcagagca cgcgaaagcg tacgaacccg cccagctgaa gtggaccatc gctgggctgc
     4021 ttcgggagtc ggctccggtc gagctcgagg cctacttggg cgtcccttcg aacccagacc
     4081 gtgttctgcg cctcgcgagc agcgcgtcgg tggtgttcga tcggtatgtt ttgtaccgtc
     4141 caactctgct gcggcaatgg tcgaatggcg cgggtggcga ctggcaggct gagctctggc
     4201 gccgggtcgt gcaacgattg ggacctcacg acctggcgag tcgaatccgc gacgcgctcg
     4261 aaacgcttcg atcgaccaag gtcgttgccg agcttccgtt cgagcgcctg cacctgttct
     4321 ccctcgagac attgccgccc ttgtttctcg agctgttcag cgcactttcc caatcggtcg
     4381 agaccacgtt ctacgtgctg gagccctcgt ccgagtacgt gtccgacgtc gatccgacac
     4441 ttggagatct gatctcgccc gacgccgccc caagcgatgg gcacccgctg ctctcttcat
     4501 tgggtcgcct cgcgagagac tttcaacaag ttctgttatc cgccgaccga gcggtccaat
     4561 acgaagccga cctcttcgag gcgccaaagc gcgataatct gctcggcgca cttcaagccg
     4621 acatcctcga gtttagaagc gccccgaaaa acagcgagcg ccgaatcatc gacccagcag
     4681 atgactcgat ctcaatccat gcgtgtacgg gtccgatgcg cgaagttcag gcacttcacg
     4741 aatgggtgca gtctgccctc gagagcgacc gttcgcttca gcccgaagac gtcgtggtaa
     4801 tgacaccgga cctcgacacc tacgcacctg cctttcgggc ggtcttcggc caggacgacc
     4861 tgcatcgtat cccttacgaa gtacacgacc gcagcacgcg cgacgacgcc gccttctacg
     4921 acgacttcca aaccatcctg gagttgctgg actcccgctt ctcggttctc gacttcgtac
     4981 ggatcctcga tgcgggcgcg ctccgtgcaa cgttcctatt cacacaggaa gagcgcgcgc
     5041 gactcgccga gttgctcgcc gcgtccggga ttcgatgggg cattgacgcc gcgcatcgag
     5101 aggcgctcga gtttcccgca gatccgctgc acacttggcg tgcggggctg gggcggctct
     5161 ttctcgggtt cgcatccacg cctgacgaca cgtcaccttt cgaaggtctt ctgccgcgcg
     5221 gtgctccgag tctcagcgac gccgagctgc tggcccggct ttcacggttg agcgaggtcc
     5281 tattcgactt ccatcgtcgt agccgagagc cgctcagcat cgaagattgg gctcgcgagc
     5341 tcgaacactt ctctgccctg ctcttcgcgg aggatgacga aagcagctca gcaatgcgaa
     5401 cgctccgaga ggcgatcgca gatcttcgag atgtcgcgac gcggactggg tacgactgtc
     5461 cgatcgcgct gaagacgttg cgaagagagc tcggcgccat actccaagag aaggcgcccg
     5521 cg
//
LOCUS       DASRZF010000302         6002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1606790, whole genome shotgun sequence.
ACCESSION   DASRZF010000302 DASRZF010000000
VERSION     DASRZF010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1606790"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VFG22_13140"
     CDS             complement(<1..239)
                     /locus_tag="VFG22_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417234.1"
                     /translation="MGCASAGAFAASAGASLGVGVGGLSVFVLHADTSASTAAVARRG
                     NPYLIIFLQLSQCRDHSIPNTAGLGFQKTKVVSNC"
     gene            238..2229
                     /locus_tag="VFG22_13145"
     CDS             238..2229
                     /locus_tag="VFG22_13145"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HET6417235.1"
                     /translation="MGEAIGHFHPKGKAPSKHTIAALEKAGGTLPLSDRRDFEEQAKG
                     FIAAPDYKKIMADAGHVAWDMERYDWLVEGEDFESIHPSTERQSKLNMNYGLYEVIPG
                     FYQVRGFDLANMTFVKGKTGWIVFDPLTAAETARAAKELVDEHLGERPVVTVVYSHSH
                     GDHWGGVRGIVDEADVRSGKVKIIAPRGFMQFAVSENVYAGNAMNRRLFYQYGVLLPA
                     SPFGYVGQGLGQGVAAGDSGLIAPTLVIEKDVEIHTVDGVKMEFKNTPGAEAPAQIQT
                     WFPASKVFWTGEDVTGVFHNIYTLRGALVRDALLWSKYINEALYRYGKQAEVMIASHH
                     WPRWGNERVQEVLRGQRDLYANMNNQVLHLANQGVTINQIHNVYQTPKGIAEQWYNRG
                     YHGSPEHNSRAVINRYLGYWDANPATLIPLSPADSAPLYVEMMGGADAIMEKGQSLHD
                     EGKYRYAQEILNKLVQAQPQNQEAKDLLADVFEQIGYQQENTGLRNSFLAGAFELRSG
                     IPTGTSPKSAGPDVVRAMSTELFLDFLGIRMDGTKAEGMEYTINLVTPDNGEQFAIEL
                     SNATLTNIKGYQAEDADLTLTINRADLEQTMMGAKPLAAQIADGTAKVRGDASILKKL
                     GSTMVDFEIGFEILPGTKSAAEVEDFNPYEVGPIDIHGE"
     gene            complement(2336..4312)
                     /locus_tag="VFG22_13150"
     CDS             complement(2336..4312)
                     /locus_tag="VFG22_13150"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HET6417236.1"
                     /translation="MLLVFVALSACSARRHAPTYEGKDLAPDVAGNSSPAAATAALNA
                     SVRERLPLSDQQDFEDARRGLIASDDELRVSTAAGKQIWNMPAYAFIDGDAPASVNPS
                     LWRQAQLNKIHGLFEVTEGVYQLRGYDLANMSLIEGDTGWIVVDPLTAKETAAMAIAF
                     ARKHLGNKPVSAVVYTHSHIDHFGGVLGIISPEEVAERNVPIFAPEHFVEEATSENVV
                     AGVAMARRSMFMMGPRLPNTERGHVDTGLGKQVAYGTFGLIPPNKIVSETGTEISIDG
                     VRFIFQNAPGSEAPSEMTFYLPDLKTFCGAEVVSHTMHNLYTLRGAKVRDAIKWSGYI
                     DEVIELFPEVDVYFGSHHWPIWGHDRIVDFLEKQRDTYKYINDQTLRLALHGATPREI
                     AEKLELPESLRTSFANRGYYGTLKHNAKAVYQRYFGWYDGNPANLDPLPPTEAGAKYV
                     ELAGGATKLLDQARAAYDKGEYRWVAEVVNHLVFADPENQDARELLAKAYDQLGYQAE
                     SGPWRDVYLTGAFELRHGGPAQGIDPAASMDMIKYAPLESFLTAMAARIDAERAEGKT
                     MTVNLVLTDVDESYVLELQNSVLRHEKRKPDPDANVTLRVTHGLFLKMLTGSAGLKDT
                     LFSDDLTIEGSRLDLLSFFRLIDKPTGTFDIVIP"
     gene            4736..>6002
                     /locus_tag="VFG22_13155"
     CDS             4736..>6002
                     /locus_tag="VFG22_13155"
                     /inference="COORDINATES: protein motif:HMM:NF023649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HET6417237.1"
                     /translation="MRTIRLFNLTLGLCVLGGCAESTTSPPPTTAAAVEERTNVYWGD
                     THLHSNYSADAYLQGNKSADPDTAYRFAKGLPAIHPGSGAKVRLSRPLDFLALTDHGE
                     FMGIIQRVISGDPLLAKTKTGKRFARWFKQGKELNVFAELIEDANAGRLNPDLTNPEI
                     NAAVWADIVDAGQQHDDPGTFTTFIGWEWTSTPGGYNLHRVVISPDGEAARNYIPFNS
                     FDSSKPEDLWAWLEKTNQETGARFVSIPHNSNISNGLMFDQVDSEGRPITAAYARTRM
                     KWEPLLEVTQMKGDSETHPSLSPTDEFADFETYEHMMKADDEADPHFSWEGGDAEVKG
                     AKVGDYARSALGRGLEIEAKVGVNPYKFGMIGATDSHTGLATYEETNFWGKYPTDSTP
                     EGKDNIVIPPKSTGWDIGASGLSGVWAEEN"
BASE COUNT         1240 a         1823 c         1842 g         1097 t
ORIGIN      
        1 tggcagttgc tcaccacctt ggtcttctgg aagcctaacc cggcggtgtt cgggatcgaa
       61 tgatcacggc actgagacaa ctggagaaaa ataatgagat acggatttcc ccttcttgca
      121 accgctgcgg tgctggcact cgtgtccgcg tgcagcacaa agacagacaa ccctccgacg
      181 cctacgccga gcgaggcgcc cgcggatgcg gcaaacgcac cggctgaagc acagcccatg
      241 ggtgaggcta tcggccactt ccacccgaag ggcaaggcgc cctcgaagca cacgatcgcc
      301 gcactcgaaa aggccggcgg cacacttccc ttgagcgatc gccgcgactt cgaggagcaa
      361 gcgaagggct tcatcgccgc acccgactac aagaagatca tggccgacgc aggccacgtc
      421 gcctgggaca tggagcgcta cgactggctc gtcgagggcg aggatttcga gagcatccat
      481 ccttcgaccg agcgtcagtc gaagctgaac atgaactacg gcctctacga ggtgattccc
      541 gggttctatc aggtgcgcgg attcgacctc gcgaacatga cgttcgtcaa gggaaagacc
      601 gggtggatcg tcttcgatcc gctgacggcc gcagaaaccg cccgcgcggc gaaggagctc
      661 gtcgatgagc atctcggtga acgaccggtg gtaacggtcg tctactctca ttcgcacggc
      721 gaccactggg gcggcgtacg cggcatcgtc gacgaggccg acgttcgctc agggaaggtg
      781 aagatcatcg ctccgcgcgg cttcatgcag ttcgcggttt cggagaacgt ctacgcagga
      841 aacgccatga atcggcggct cttctaccag tacggcgtgc tcctgcccgc gagcccgttc
      901 ggctacgtcg gacaagggct cggccagggc gttgcggccg gcgattcggg gctcatcgcg
      961 cccaccctcg tcatcgagaa ggacgtggag attcacaccg tggacggcgt gaagatggag
     1021 ttcaagaaca cgccgggagc cgaggcgccg gcccaaatcc agacttggtt tccagcgagc
     1081 aaggtctttt ggaccggcga agacgtcacg ggtgtatttc acaatatcta cacgctccgc
     1141 ggcgcgctgg tgcgggacgc gcttctctgg tccaagtaca tcaatgaagc gctctaccgg
     1201 tacggcaaac aggccgaggt gatgatcgcg tcgcatcact ggccgcgctg gggcaacgaa
     1261 cgcgtgcagg aagtgcttcg tgggcagcgc gacctctacg cgaacatgaa caaccaggtg
     1321 ctgcacttgg caaaccaggg cgtgacgatc aaccagatcc acaacgtcta tcagacgccg
     1381 aagggcatcg ccgagcagtg gtacaaccgg ggctatcacg gctcgcccga gcacaacagc
     1441 cgggccgtga tcaatcgcta tctcggatac tgggacgcca atccggctac gctgattccc
     1501 ctttctccgg ccgactcggc accgctttac gtggagatga tgggtggcgc cgacgcgatt
     1561 atggaaaaag gtcagtcgct ccatgacgaa ggaaagtacc gctacgccca agagatcctg
     1621 aacaagctcg tgcaggcgca gccccaaaac caagaggcga aggacctgct cgccgatgtc
     1681 ttcgagcaga tcggctacca acaggagaac accgggctgc gcaacagctt ccttgcgggt
     1741 gcgttcgagc tgcgctcggg tatcccaacc ggtacatcgc caaagtcggc cggcccagac
     1801 gtcgtgcgcg ccatgtcgac ggagcttttc ctcgacttcc tcgggatcag gatggatggc
     1861 acaaaggccg aaggcatgga gtacacgatc aacctggtga cgcctgacaa cggcgagcag
     1921 ttcgcgatcg agctctcgaa cgccacgctc accaacatca aaggctatca ggccgaagac
     1981 gcggatctca ccctcaccat caaccgagcg gacttggagc agacgatgat gggtgcgaag
     2041 ccgctcgcgg ctcaaatcgc cgatggcacc gcgaaggtcc gcggcgacgc gtccattctc
     2101 aagaagctcg ggtcgaccat ggtcgacttc gaaatcggct tcgagatcct gcctggcacc
     2161 aagagcgctg ccgaggtcga ggatttcaat ccctacgagg tcggaccgat cgacatccac
     2221 ggcgagtagc gctccggcaa tgctcccgag gcggccgagc aatcggccgg ccgcgacacg
     2281 cagaccgtca acgcaaacaa gaggagcaga tgctgaaggg cagtctgctt gggagctacg
     2341 gaatcacgat gtcgaaagtc cccgttggct tgtcgatcag tcggaagaaa gacagcagat
     2401 cgaggcggct tccttcgatg gtcaagtcgt cggagaacag ggtgtccttg aggcccgcgc
     2461 tgccggtcag catcttgaga aagagaccat gcgtgacgcg caacgtcacg ttcgcatccg
     2521 ggtcgggctt gcgcttctcg tggcgcagga ccgagttctg tagctccagg acgtagctct
     2581 cgtcgacgtc ggtgagcacg agattcacgg tcatcgtctt tccttcggct ctttcggcat
     2641 cgatgcgcgc agccatggcg gtgagaaagc tctccaaagg cgcgtacttg atcatgtcca
     2701 tagaggccgc tgggtcgatc ccctgtgccg gtccaccatg gcggagctcg aacgctccgg
     2761 tcaggtagac gtcacgccaa ggtccggatt cggcttggta gcccaattgg tcgtaggcct
     2821 tggcaagcag ctcgcgggca tcctgattct cggggtctgc aaacacgagg tggttgacca
     2881 cctcggcaac ccaccgatac tcgcctttgt cgtacgccgc tcgcgcctgg tcgaggagct
     2941 tcgttgcgcc acccgccagc tccacgtact tcgcgccggc ctccgtaggc gggagcgggt
     3001 cgaggttcgc agggtttccg tcgtaccagc cgaaatagcg ctgataaacc gctttcgcgt
     3061 tgtgcttgag cgtgccgtag tagccgcggt tcgcgaagga cgtgcgaagc gattcgggca
     3121 gctcgagctt ctcggcaatt tcgcgcggcg tggcgccgtg cagcgcaagt cgcagggtct
     3181 gatcgttgat gtacttgtag gtgtcgcgct gtttctcgag gaaatcgacg atgcggtcgt
     3241 ggccccagat cggccaatga tgactgccaa agtagacatc cacttcaggg aagagctcga
     3301 tcacctcgtc gatgtagccg ctccatttga tcgcatcgcg gacctttgcg cctcggagtg
     3361 tgtagaggtt gtgcatggtg tgtgacacca cttccgcccc gcaaaaggtt ttcagatcgg
     3421 gcaggtagaa cgtcatctcc gagggggcct cggaaccagg cgcgttctga aagatgaacc
     3481 tcacaccatc gatcgagatc tctgtaccgg tctcgctgac gatcttgttc ggagggatga
     3541 gcccgaaggt cccataggcg acttgctttc caagcccggt gtcgacatga ccgcgctcgg
     3601 tattcggcaa gcgcggaccc atcatgaaca tcgagcgccg cgccatcgct acccctgcga
     3661 ccacgttctc gctcgtcgct tcttcgacga agtgctcggg cgcgaagatc ggcacgttcc
     3721 gttctgcgac ctcttcgggt gagatgatcc cgagcacgcc tccgaagtgg tcgatgtggc
     3781 tgtgagtata gaccacggcg gacaccggct tgttgcccag atgcttgcgc gcgaaagcga
     3841 tcgccatcgc tgcggtctct ttggccgtca agggatcgac gacgatccaa cccgtgtctc
     3901 cctcgatgag agacatgttc gcgaggtcgt agccgcgcag ctgataaacg ccctcggtca
     3961 cctcgaacaa gccgtggatc ttgttcagct gggcttgccg ccacaggctc gggttcacac
     4021 ttgccggggc atcgccatcg atgaacgcgt aggctggcat gttccagatc tgcttccccg
     4081 ctgcggtgga cacccggagc tcatcgtcgc tggcgatcaa accacgtctc gcatcctcga
     4141 agtcttgctg gtcggaaagc ggcaggcgct ctcgtaccga cgcattgagc gctgccgttg
     4201 ccgctgcagg tgacgagttg cccgctacgt cgggcgcgag atctttgccc tcgtaagtgg
     4261 gtgcgtgccg tctcgcgcta caggcgctca gcgcgacgaa gacaagcagc attgagactg
     4321 atatcgagtg ttgcacgcgg tcctgtcctc ccaccgaaaa cgacgaattt agctcgttca
     4381 cgttctggga aatgtagata agtctacaat cggatgttgg ttcgaatgcc tcctcatgga
     4441 ggtcgaggtc ttcgtgcgga aacgtgttga ccgctcgcga agcgccgcgc cttcttcttc
     4501 cgcagcgggc tgcgcgctca gatgcgagct actcacaagc ccgcagagaa gcgacccacc
     4561 aaacacgatc gcgaaccact tggccgtagc gggttgccac aagcgattcg cggggagctt
     4621 cgatttctcg tcgtttatgg tcgcgttgac actaccgaaa gaaatccgta gtctcgcgca
     4681 aggtgcggtc aaccatagcg cctactgggc gctactcgta gacggagcaa gagacgtgcg
     4741 aacgattcga cttttcaacc tgacgttggg cctgtgcgtt ctaggtgggt gcgccgaaag
     4801 caccacgtcg cctccaccca cgaccgccgc cgcggtggaa gagcgaacga atgtgtactg
     4861 gggcgacacg cacctgcact cgaactactc ggccgatgcg tacctgcagg ggaacaaatc
     4921 agcggacccg gacacggcat accgcttcgc gaaagggctc cccgcgattc atcctgggag
     4981 cggcgcgaag gttcgtctgt ctcggccctt ggacttcctc gcgctcaccg atcacgggga
     5041 gttcatgggg atcatccagc gggtgataag tggcgacccc ctcctcgcga agaccaagac
     5101 cgggaagcgc ttcgcccgtt ggttcaagca gggcaaggag ctcaacgtct tcgcggagct
     5161 gatagaggac gccaacgcag gaagactgaa tccagatctg accaatccgg agatcaatgc
     5221 ggcggtgtgg gccgatatcg tcgacgcggg tcagcagcac gacgacccgg gaacgttcac
     5281 gaccttcatc ggatgggagt ggacctccac gcctggcggt tacaacctcc atcgcgtcgt
     5341 tatctcgccg gatggtgaag ccgcccgaaa ctacatcccc ttcaacagct tcgatagctc
     5401 gaagcccgaa gacctctggg cttggctcga gaaaaccaac caggaaacgg gcgcgcgatt
     5461 cgtttcgatt cctcacaact cgaacatctc gaacggcttg atgttcgacc aggtcgatag
     5521 cgaaggccga ccgatcaccg ccgcgtacgc gcgcacgcgg atgaagtggg agccactgct
     5581 cgaggtgacg caaatgaagg gagattcgga aacgcacccc tcgctctcac cgaccgatga
     5641 gtttgccgac ttcgagacct acgagcatat gatgaaggca gacgatgaag ccgaccctca
     5701 cttcagttgg gagggtggag acgccgaggt caagggtgcg aaggtcggcg actacgcgag
     5761 gtctgcgctc ggccgcggcc tcgagatcga ggccaaggtc ggcgtcaatc cgtacaagtt
     5821 cggcatgatc ggcgctacgg acagccacac cggcctggcg acctacgaag aaacgaactt
     5881 ctggggcaag taccccaccg attcgacgcc cgaaggcaaa gacaacatcg tcatcccgcc
     5941 gaagtcgacg gggtgggaca ttggcgcttc ggggctttcc ggcgtctggg ccgaggagaa
     6001 ta
//
LOCUS       DASRZF010000303        24525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2983203, whole genome shotgun sequence.
ACCESSION   DASRZF010000303 DASRZF010000000
VERSION     DASRZF010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 24525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24525
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2983203"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1911
                     /locus_tag="VFG22_13160"
     CDS             <1..1911
                     /locus_tag="VFG22_13160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein meaA"
                     /protein_id="HET6417238.1"
                     /translation="TNLAKGQTGLSVAFDLPTQTGYDADDPLARGEVGKVGVPVSHVG
                     DMRALFDGLPLDQMNTSMTINATAVWLLSLYIAVAEDQGVDHHVLRGTTQNDIIKEYL
                     SRGTHIFPPAASMRLITDLITYTVDEVPKWNPINICSYHLQEAGATPTQELAYALATA
                     IGVLDAVKATGRVPESEFGKVVGRMSFFVNAGIRFVEELCKMRAFVELWDRVTRERYG
                     VTDPKERRFRYGVQVNSLGLTEQQPENNVIRIVLEMLAVTLSKDARARAIQLPAWNEA
                     LGLPRPWDQQWSLRAQQILAYESDLLEYQDLFNGSKVVEAQTARLIDSAWAEVQEVLK
                     RGGAVEAVETGYIKQKLVESNAARLRAIERGDQIVVGVNKFIEGEPSPLTENLDEAIL
                     TVDPEVDASQRAALARWKEERDQGAVDRALAELQRVARTKENMMAPSIAAAHAGVTTG
                     EWANTLREVWGEYRAPTGVAGATVKTSGTGPAAENVARLRRRLSELERRMGRRAKLLV
                     GKPGLDGHSNGAEQIAIKARDVGFDVVYEGIRVSPEQIVASALEEGVHLIGLSILSGS
                     HLALVPEVVRRLREAGLGDIPVVAGGIIPEADAARLRSEGVAAVYTPKDFELDKIMSD
                     LIDLVDRQHAAE"
     gene            1937..2905
                     /gene="meaB"
                     /locus_tag="VFG22_13165"
     CDS             1937..2905
                     /gene="meaB"
                     /locus_tag="VFG22_13165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HET6417239.1"
                     /translation="MSSEKAAVSRALNLVEDRRPGSQAQVAALLAALKDAPKAAAGHR
                     VGLTGPPGVGKSTLTSALARGLRQHGRTVGIVAVDPSSVRSGGSLLGDRARMSFDPGD
                     AGLFVRSLATAGEVGGLAYAANSAVRVLAAAYDMVVVETTGVGQSEIDIVHVVDTVVL
                     VIQPGSGDVLQFLKAGIMEIPDILVVNKADHEELARRAVADLHGALRTAHAVGADGGQ
                     DADWETPIVATSATAGTGIDALVEALDRHRRHLESTLSVRRRQGEVHWALDLFARKHG
                     EHGISTLGGRDTLKARAERDLDRRETPLSLCEDLSRDYVAALGKTP"
     gene            2913..4133
                     /gene="ccrA"
                     /locus_tag="VFG22_13170"
     CDS             2913..4133
                     /gene="ccrA"
                     /locus_tag="VFG22_13170"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR01751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HET6417240.1"
                     /translation="MSERPNIVPIGQIPELGVVPEKMHAFVIRPERFGEPMQSFKEEE
                     ARVWDLGPGEVLVQVMAAGINFNAIWAGLGKPVNILKGHGLDYHIAGSDASGVVWAVG
                     PGVHDHKVGDEVVLHCNHLVNPGTNDLQTIWGYETPDGSFAQFTKVQAQQVLPKPAHL
                     SWEEASSYGLTYYTAHRMLVDRAKVQPGEDVLIWGAGGGLGVFAVQLCKLMGARAIAV
                     VSSDDKAELVMQLGAHGFINRKDFPALQYKDGMTPEEEKAHNADLKGFGKRIWEILGE
                     RKGPEVVFEHVGKATFPASVFLASKYGRIVICGATTGYDLNFDVRHLWMRQKSIIGSH
                     FADADSASRANRLVIQDKIKPVMTQLFTWDEIPKAHQLMYENKLHGTVACLVGAPRPG
                     LKNLEETLAAIANG"
     gene            4142..5755
                     /locus_tag="VFG22_13175"
     CDS             4142..5755
                     /locus_tag="VFG22_13175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6417241.1"
                     /translation="MQEVINAIEGVYKRAHDNVKALVSDGERISAKKLNQHQLAAHAV
                     AYLATELEACRQLADWAERVGGEYEGKVARAYIGEVARSIVGGVDLGPCESISLSEVG
                     ITDGDLRETVLKPNVVAFANEHSNGQVYIELATHARNHGFPNPGYGDEMLDEIRSQFS
                     KFVDAVVIPRAQDIHRNDVLIPMDILDQMSELGVFGLTIPEEYDGLGMSKVAMCVVTE
                     ELSRGYIGVGSLGTRSEIAAELIMGGGTEEQKRKWLPKLASGEVLSTAVFTEPNTGSD
                     LAHLGTRAVKQQDGSYVINGQKTWITHAVRADLMTVLARTNADEPGYKGLSMFLAEKT
                     RRTGEDGEAEFVDEGLTGTEIKVLGYRGMKEYELSFEDFELPADSLLGGEEGAGFKQL
                     MRTFESARIQTAARGVGVAQAALEDAILYATEREQFGGPIFQFPRVARKIGRMICRIQ
                     AGRQLTYFSAREKDQEKRCDLEAGMAKLLATRSAWESADANVQIHGGNGYAEEYTASR
                     LLVDARVLSIFEGANEIQAHVVARRLLES"
     gene            5789..7477
                     /locus_tag="VFG22_13180"
     CDS             5789..7477
                     /locus_tag="VFG22_13180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET6417242.1"
                     /translation="MSLDRVGERRRPIRIAFCLVLAVQVLLAVWGVADQWTRGHNGWN
                     GAAYQNAARNTLRWDVLFPLQYDTANVPPKPEQLYTHAPLALHLHNVVSVKIFGDREL
                     SIRLVAGFWSVAALIMLFAVVRRLWDDAHALAASALYVALPINAIYINMANHSAGFIF
                     WSLLALYLYVQRPRGKLWPWPRFVFFLVVIAIAATWDWPAYYIAFCIALHWAYSVKKS
                     SEPFAQLLVFCVWIGFWFVGHFALVELLTGNLDELTGTFNARRALSWDRFRTHLLVVP
                     ELMFTAPILGISAAWAGTWLLRLARGTARARDLVPLAFLFAGLVHYWTFRWSAIVHSY
                     WAWPMLPAVAIAVTASLFTMARWIRARAGAAAGWSVLLLLVPLAIRIVQIVPEARYVG
                     GSMWFFTPVRGAVDTYDSGRPELRFATMVKAWTDRDTGVQLHTSLKPLRLEPRFDITL
                     DRVTHRWSQHPRKEIPNGQGAKAWVFIAAVDAFSDEERAAFASRHPYRQYGDYFMLDL
                     RSEGRDIEIWGLTPLPFDLRWRLLRSAFEPPIRASRQIASEQALDEKVAALDAD"
     gene            7467..9491
                     /locus_tag="VFG22_13185"
     CDS             7467..9491
                     /locus_tag="VFG22_13185"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HET6417243.1"
                     /translation="MRTSPWTSPLELWASAYLGCTAVLLLGLLPEGEVSFALLSQLCW
                     LALIGSIPTGLVLGAASRVATSSTAVVFVSTLAVISVSSLIAWEHTDFLLSGPYWLLH
                     QQREVARIGMLAAVATACGGGWLWLLLGSRIQDRIPLTAWFALTLTAVALLTSAIVRY
                     RAYDYSMAQLVFPAGVLGAAVVYRLFRRSRYRPLVLGAAMACLLCGAGSRFDPSMVAT
                     GQREVIAQSRAGALTTVYVLPQFRTDEDWVVNGVACPDPRPSLETSAIGIAPERRRNV
                     IVITVDALRKDVVGGIVNGRAVTPALSSWARTAVSFENATSTYPATLFAVGSAFTGLS
                     PAELYLSPSLPETIFTRTRAIIDRQVAILPDVSWFRLPIVGQFLAPGVETEFAPSDAA
                     ATDALIERLRQARNDGATVMAWLHFYSPHDPYVPQSDFSFGKGKRNAYLSEVAYFDRQ
                     LGRLMNYLDQEHWLDDTLVIFFSDHGEALGERSYWGHHVYLNAWMIDVPLMLWHSDLV
                     PAAPLVGVSLADVAPTVLHFLGLPLPSDIAAESLFTLDPNQVGRPTFSEAFPVRGRQL
                     FESFRLPALDDSAIRARLRSIRQSSEGYEPKGAITQDRYRLIHHRGADATLFYDHELD
                     PGERSSQGVGNRERAELLRSELERWEQEQLRRIRCRLQLSADPAATSNPR"
     gene            complement(9454..11631)
                     /locus_tag="VFG22_13190"
     CDS             complement(9454..11631)
                     /locus_tag="VFG22_13190"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET6417244.1"
                     /translation="MALCLGAPWLTRGLLLREKGMLLALADLRGFLSDTSIALLVMGA
                     VGLLFSLGRYWGRALGLLVLIAFVVLSFAMYEFISVFDSLYALSHAGFLTDSVFLGGS
                     AGRLAHPFVFGSFLVLAIFAAFSARRPKERWWRWWGLAFATSVLGQVAIPWSHQQDEW
                     RQRHAVYANLAIVGASPNEESGTISAEVRDVFRSNLHGERWIGPFLDRPNVLLIMVEG
                     ASGAYLPSVAAAQGVESTTRMPRLDALAQEHIVFTQVISHQRQTNRGEYGILCGDYPK
                     LLTDQSKMSEQAYGSARLCLPQVLRKLGYSTAYIQAAPLGFMLKDQFMKKAGFDQLVG
                     DPWFEKSYARTDWGVDDKAFFEQSIEKVVALHEASAPFFATLLTVGTHHPFTIPADAG
                     GDGARSRQERAFLWADDALAVFLDELQRRGVLRDTVVIITSDESAGLARAESATQRLL
                     SQSWSFVVVMLPIPQRKRIDTLQAHVDTALSVTDLLGIEGEARQFLGRSWFRDYAVPR
                     RLFAGNTYARRVIMWEASGSAVVCSESFQDCTRTIPERSLVFGPSRRSEPADGRERQL
                     LAEVARLTRSGRRDMTQTEALDLLAQEEITVRASDGKKLLIGGQYLRVPAGTNLEVVL
                     DLEVLGDEAVVDFHQDVFLNGYSKFERKGFLVRNGERWKLRYEIGVPDDSGQLVVQLY
                     ATTVAGQAARIRFNEARLGMSRAAIRSESVRVIEDSTSQPDPR"
     gene            11712..12311
                     /locus_tag="VFG22_13195"
     CDS             11712..12311
                     /locus_tag="VFG22_13195"
                     /inference="COORDINATES: protein motif:HMM:NF017855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HET6417245.1"
                     /translation="MAKGFAPAAERNRQPILDVLRRVLPPRGLVLEVASGTGQHAVFF
                     SEQLRALRWQPSDVSPDALRSIGAWVAELDRSNLLAPFELDVRWPAWPLAEADAVLSI
                     NMIHISEWETSEALFEGARKLLAPGAPLITYGPYRLHGEHTAPSNAAFDESLRSRNPR
                     WGVRDIDELTKLADRTGFTLEERVQMPANNMTLVWTRDA"
     gene            complement(12308..13300)
                     /locus_tag="VFG22_13200"
     CDS             complement(12308..13300)
                     /locus_tag="VFG22_13200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HET6417246.1"
                     /translation="MTLFYAITSVIVGFIAVVWGADRFVHGAAGLASNLGLSRLVIGL
                     TIVAFGTSAPEMLVSGMAAAAGSREMGIGNAIGSNITNATLVLGAAAFAQPLSVHSRL
                     LIREIPVLFLIMGLAWAFLWDGELSRVEGFVLFAGMFALIVWISLQGREERSRDISDP
                     ALAFEDDQIPSSLPTPRAVAVLSVGLLVLLAGSRVLVWGAVGIARDLGVSELVVGLTL
                     VALGTSLPELAASVAAARRGEPDIAVGNVVGSNMFNLLGVLGLPGIIAPGEVDRSVIF
                     RDFPIMVGATLLLLLMAVNERSHIGRKRGAALILVFVLYFVMLFWNPSLPHMPS"
     gene            13433..14194
                     /locus_tag="VFG22_13205"
     CDS             13433..14194
                     /locus_tag="VFG22_13205"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HET6417247.1"
                     /translation="MAERLEGKVAIVTGASRGIGAAIATQMAEEGAKVVLVSRKIEGL
                     ETVAGEIRDKGGDATAIACHVGHRDQREDMLEKALKAYGKVDILVNNAATNPHFGPML
                     TIDGGAWDKTFEVNVKGYFGMIQLVAGHLQQRKARGSVVNVASVVGLMAAPMQGVYAM
                     TKAAVISMTKTLAMELGGAGIRVNAIAPGLIETKFAQVLVDNDEIRSSIVNRTAAGRV
                     GQPRDIAGGAVFLASDESDYVTGDVMVIDGGWTLA"
     gene            14207..18634
                     /locus_tag="VFG22_13210"
     CDS             14207..18634
                     /locus_tag="VFG22_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417248.1"
                     /translation="MMGKPPPSPGEVARKRDLVESRIVNLRRELESETEPRTQAAILY
                     EVAALHEHELEEISEAFSCYGEAYASAPSFQPALIAQLRLAERGDIGCDSSAITSSLA
                     AHATSPSVSAGAFLDLALRSEDWASHLREAIARSPEPAVPALIMEWLAEARGDQDSLR
                     LAVHTQALHASEPSLAAALFVDLALSEIEAGRPEEAIEALEHACEARALLWQARSLQL
                     RIAREHGRWDVLIRAATSMARLLEAASESDEPTDPLELPVPPVERLPMAAFLWQEASA
                     CSDVRLDDADSAAQYIEAALRLFPDRSSIRLKSLRLADKRGDDLAFQSACDWFRANAP
                     GDPAFVAQGLRRALAEGDGSQTQAIAQNAATVFPQSEYLQAARDVAAIRTGSVADRVE
                     HLQECAESAEGEARARCLWHAAQLANSSPGTEPDAQVLYSKAIAAAGGSKTRIVREAL
                     GAALRAKQPDRILERCAELSQCEVDPAERSSIGFTEYYVTQHGSSADEPAQVVLRDAV
                     QNPDSREWAPGLARAHAAWSGNEALLAEAHEALAGATDGESKLGHLSAAGQAHARSGN
                     WSAAERVLRQASAAAPDDDYVLSLLDGVLREGGHPEDVVSLARERVQGPSGTQLGEVS
                     LLLAGAAAERSENLHAAEQAYEQAVLEDPSSPSPALALLDIARRRGDAEATLRAYERL
                     SESNLGGGVPELYALLRGDALAFRETVGAETSDAYERGLEHPTSAVAAAVALMSLPRR
                     LTSAEQRAASEEVLADVGESSENIAEEFETAYGALRASLAEESSSSGDAWLQLAALAP
                     SESLRAAALLQGLRATRIAQGAGAADEIFMLAQDAGVLSESQSDATIAIDEALAPGDD
                     AEIRAHALEHKRRHSEAIGRGALDAAYCRALVEAHRGAEAVALLSAAVNERPDDLSVW
                     EMLRDAARQAGDWPSVAQACERLAPFVDGSLKADLLEEAGVVRLDHLEQYQQARDLFR
                     AALHEDPRRDVAFRRLHDLLTAQEDAEALETLVSERLALSDPEDRQELLYERARLLRG
                     FSDRPGALEVLDELIASEPDHPGALALAAEIHVSLEQWAEAVACLRSLAMAGIPEEQR
                     RVAHLGAADFLETHLGAKSEALQELRAIEALDLADAQIWTRIGALELELENRGAAIDA
                     YQRALDAEPTNAAAISILIELLDGAARNDALVAFEQAVWQRIDEGRLDAALLEGLADA
                     ASWRGDARRTAAVHAARVALGLSPSSGVSNFDLDQVSVAALWDVNADELLRDVLRSAG
                     PALTKSRWRTKRAAPDDPICTELERICQRFGTRAGLVKLSDEPDATLEAELGDSAVDW
                     VVPRGSKAGLDAAGRFAAGRLAWGTPHGAARLLDDSPEKVAGKLAAILRAARCELAPG
                     GPQLPAAQIKLRRIVRKAVKDRVGDATLTAPALITFARSLQRSADRAGLLTSGNIAVA
                     LSKLLGGNPSLNALRSSPRGLDILRFWIHADSPLWGNDV"
     gene            18627..23333
                     /locus_tag="VFG22_13215"
     CDS             18627..23333
                     /locus_tag="VFG22_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417249.1"
                     /translation="MSEDDLSAASRESEAPSFDFDEDAIATLLDTFVETRTGPQAKRV
                     SRPPDPSDSGAQAAQRTVFQVRSRDESLPLVGERPEAKKRRIELLEALAGRAVGSAKA
                     RLLTSAAELHERLGNAEAAERDYQMALGADARDVVVVRAMRRCAMNREDWPAAADALE
                     KEAGLELSSSERGSALKLLASIQLWKLRDPAAGEQAASHAVALRDEDFSALVLLASAR
                     MARGDAGGAADALSSAAEHWPQSEARGLIHLHAAELMEDAGALESAQRAYQRVLELSP
                     SRLAAHLGVVRVSRALGAWEPAIEALHQAAEVAPAPVAAALLRTAACVRMRTGGPKEL
                     VTLLEGAVDVASRWTLAEAAALANELERAIEAFDSDVPGLSPEARTMSSARRARFHAE
                     LQDPARFERAASESGSARSLTPYLRACHRLLSSNEEDEGRLRRMLETVSDEASSPSSR
                     MVRADDAARDGNGARFFQALEHELDEAPDHLAAGAALAWVEMAEHSARIDHRSALLRA
                     EERMPQNPLIAQALLLDDDDVSANARRWSNAGRATEGDRRAFAFTMAARLASPETEAA
                     MGASDEALAASTDYWPTLWELEDQIGTPDARAASAVRQAELDAANEIPDMLRASMWTT
                     SASKRLELAAATLDRAAPDPLLLEHLFDAAGTSSEAAGDLMMLAADRMAGLPYFERAA
                     DSYRLAGLPSQAAKVLRAASAKRPEDPMIRVQRKDAELEACEFARLADSAMRRAREAR
                     TDEEELGAFADMAEVDRLARRDMQSARLALQSIAESRPDHIPTARALEWDALRENDTE
                     RIRSSARRLLEILPADSAERLARQRLILESLSSDPDIMRIDLDRQLGRIEEPLAADPG
                     LARQVLGAAYAGSQPLRCVPALEALRSTLGDELEEGAVALDTARVLERIGDHERALQT
                     LDAAGSHPLARESEARLLHTAKRWEEAAATYRDAATRAKDSHRAAALWREAACILEEE
                     LGNLDAAVDAWVAAANCDIRYLDVYRRLAALYRRQGELDRLASLTDRRIDAGADTPTL
                     VSLLVDKARQRRERGDLEGVIAALDECLELDPHHFAALKELVDTHRGAEDWQGAAEAL
                     IRIARLKRSVEEQSWAFTQLAEIYDVHLHDLSRAEASLRQVAKLAPTHLETLDRLASV
                     LSRENKALEAARLLEELIRRTTGETELRDYRIRLAVAVESAGQPRQAELLLESLRAEQ
                     PTEIDVILAAADHYQRQGAAPAEAMHLNRAVTDLRSAIDADPGSEALWTTLVRVLHRR
                     HGPGPASCAASAAIALGHPASLFDGDVSRQNEALGEPKVPMSKLVDSIVAPVGLPQTL
                     RRLFALSEQSFDKALPFDASAWHLRRPSGPFMSLVEEAGAVAEALGISEPRLRVTHVA
                     PAACMPVSGDPPTIVVGGKLHEITSARERVFLFARALKVASNHLAPALRARPEELDVA
                     LLALLQAHDASRGEHPRARELQDLRKKLLKSVPRRWRDEVESLVLELRGNASFSTRSV
                     PMAISELGDRAALTLTGDVPSAVDALLKIAGHDVPASDSGRLGAILENREAWAMVRFA
                     IADAHFEARAQAGVDP"
     gene            23338..23931
                     /locus_tag="VFG22_13220"
     CDS             23338..23931
                     /locus_tag="VFG22_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417250.1"
                     /translation="MRLGFIGPAKTDTAALERAAKLLICDLEVDTLIYLGEDDALREF
                     IAAHTNGGSEEPIEQQVAEVAATGSPEQITEVLRRLRGARYLGKLRVVPAPPIRAMEM
                     LDDRIVLIVRNKSTIGEEDVINSNLVVYGDGNELMFKRFGPRCFFSPGPLDHGHVGVI
                     DDQSETGGVVLEAIDVNGDLRWREPIQGRGAKVMVAP"
     gene            23928..24491
                     /gene="nadD"
                     /locus_tag="VFG22_13225"
     CDS             23928..24491
                     /gene="nadD"
                     /locus_tag="VFG22_13225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HET6417251.1"
                     /translation="MTAERIAIYGGSFDPPHLGHVLSVAWALSAAEIDSVWVIPTWKH
                     AFDKEHRASFEERLALAELAFAPFHQVLVSDVERRLGGVSRTLVTLEALQAERPGAVF
                     RLLIGADVLPTTSRWHRWEEVARLAPPLVVGREGYPAPEGCPISIPNVNSTEIRLRLS
                     NAGEIRGLVPTKVVEHIRSHGLYQGSP"
BASE COUNT         4637 a         7842 c         7864 g         4182 t
ORIGIN      
        1 accaacctcg ccaagggtca gaccgggctg agcgtagcgt tcgatctgcc aacacagact
       61 ggctacgacg ctgacgatcc gctcgcccgt ggtgaggttg gcaaagtcgg cgtcccggtc
      121 tcacacgtcg gggacatgcg cgctctgttc gatggcttgc cactcgacca gatgaacact
      181 tcgatgacga tcaacgcgac ggcggtctgg ttgctgtcgc tctacatcgc cgtcgccgag
      241 gaccagggcg tcgaccatca cgttctgcgc ggcaccactc agaacgacat catcaaggag
      301 tacttgtcgc ggggcacgca catctttccc ccggcggcct ccatgcgact cattaccgac
      361 ctgatcacct acacggtcga cgaggttccg aagtggaacc caatcaacat ctgtagctac
      421 cacctacagg aagctggtgc gacgccgaca caggagctcg catacgccct cgccacggcg
      481 atcggtgtgc tcgatgccgt caaagcgacc ggccgagtgc cagagagcga attcggcaag
      541 gtggtcgggc gaatgagctt cttcgtgaac gccggcattc gattcgtcga agagctctgc
      601 aagatgcgcg cgttcgtcga actatgggac cgcgtcacgc gggaacgcta cggcgtaacc
      661 gatcctaaag agaggcgctt ccgctacggc gtccaagtca attcgctcgg actcacggaa
      721 caacagcccg agaacaacgt catccgcatc gtcctcgaaa tgctggcggt cacgctcagc
      781 aaggacgcgc gtgcgcgtgc aattcagttg cctgcttgga atgaagcgct cggcctgcca
      841 cggccctggg accagcaatg gtcgcttcga gcgcagcaga ttctggcgta tgaaagcgat
      901 ctcctggagt accaggacct cttcaacggc agcaaggtcg tcgaggcgca gactgcacgg
      961 ctcatcgact ccgcatgggc tgaggtacaa gaagtgctca agcggggcgg ggcagtggaa
     1021 gcggtcgaaa ctggttacat caagcagaag ctggtcgagt cgaacgctgc gcggcttcgt
     1081 gcgatcgagc gcggcgatca gatcgtcgtc ggcgtcaaca agttcatcga aggcgagccc
     1141 tctcccctga ccgagaatct cgatgaagcg attctgaccg tggatccgga ggtcgatgcc
     1201 agccagcgcg ccgccctcgc ccgctggaag gaagagcgag accagggcgc cgtcgaccga
     1261 gcactggcgg agctgcaacg cgtggcccga accaaagaaa acatgatggc cccgtcgatc
     1321 gcagccgcgc atgcgggtgt gaccaccggc gagtgggcga acacgcttcg cgaagtctgg
     1381 ggcgagtacc gcgcgccaac cggcgtggct ggagccacgg tgaagacttc cggcacgggg
     1441 ccggccgccg agaacgtcgc gcgtctgcgg aggcgtctga gcgagctcga acggcgaatg
     1501 gggcggcggg cgaagctctt ggtcggaaag ccggggctcg atggtcactc gaatggcgcc
     1561 gagcagatcg cgatcaaggc gcgcgatgtc ggcttcgacg tcgtgtacga ggggattcgt
     1621 gtgtctcccg agcagatcgt ggcttcggcg ctcgaagaag gcgtacatct gatcggcctc
     1681 agcatcctga gcggttccca cctcgccctg gttcccgagg tcgtccgacg acttcgcgag
     1741 gcgggtctcg gcgacatccc ggtcgtcgcg ggcggaatca ttccagaggc cgacgcggcg
     1801 cggcttcgga gcgaaggagt tgcggctgtc tatacgccaa aggacttcga gctagacaaa
     1861 atcatgagtg atctgatcga tctggtggac cgccagcatg ccgccgagtg atcctaccga
     1921 gcacgcgcag cacgtgatgt cctcggaaaa agccgcggtg tcccgtgcct tgaatctcgt
     1981 cgaggatcgc cgacccggtt cacaggcgca ggtcgctgcg ctgctggccg cgctcaaaga
     2041 cgcgcccaag gcggccgcag ggcatcgcgt cggactcaca ggtccccctg gcgtcggaaa
     2101 gagcacgctg acgtcggcgc tcgcccgcgg actacggcaa cacggccgca ccgtcggtat
     2161 tgtcgccgtg gatccttcga gcgtccgctc gggcggctcg ttgctcggcg accgcgcgcg
     2221 gatgagcttc gacccgggcg acgcgggctt gttcgttcgc tcactggcga cagccgggga
     2281 agtgggcggc ctggcgtacg ccgccaattc cgccgtgcgc gtcctcgcag ctgcttacga
     2341 catggtcgtg gtcgaaacta cgggcgtcgg gcaatccgag atcgacatcg tccacgtcgt
     2401 ggacacggta gtgctcgtca tccagccggg aagcggcgac gtgcttcagt ttctgaaggc
     2461 agggatcatg gaaatccccg acattctggt cgtcaacaag gccgatcatg aggagctggc
     2521 gagacgcgcc gtcgccgacc tccacggcgc acttcgaacc gcacatgcag tgggggctga
     2581 cggcggccag gacgccgact gggagacgcc aatcgtagcc acgagcgcga ctgcgggcac
     2641 gggtatcgac gcattggtcg aagcgctcga tcggcatcgg cggcacctcg aatccacctt
     2701 gagcgtgcgt cgtagacagg gcgaggtgca ctgggcgctc gaccttttcg cccggaaaca
     2761 tggcgaacat gggatttcga ccctaggtgg gagggatacg ttgaaggccc gcgccgaacg
     2821 agacctcgac cgccgcgaaa cgccattgag cctttgcgaa gacttgagcc gggactatgt
     2881 agcagcgctc ggcaagaccc catgaggaag acatgagtga acgtccaaac atcgtaccga
     2941 tcggccaaat cccagagcta ggcgtcgtgc ctgagaagat gcacgctttc gtcattcgac
     3001 ccgaacgatt cggcgagccg atgcaatcct tcaaggagga agaggctcgc gtctgggatc
     3061 tcgggcccgg cgaggtgctc gtgcaggtca tggcggccgg catcaacttc aacgcgatct
     3121 gggcggggct cggaaagccg gtgaacatcc tcaaggggca tggtctcgac taccacatcg
     3181 ctggatcgga tgcgtcgggc gtcgtctggg cggtcggccc cggggtccac gatcacaaag
     3241 tcggcgatga agtcgtcttg cactgcaacc atctcgtcaa cccgggcacg aacgacctgc
     3301 agaccatctg gggctacgaa acacccgatg gatcgtttgc gcagttcacc aaggtccagg
     3361 cgcagcaggt ccttccgaag ccggcacacc tcagctggga agaggcttcg agctatggcc
     3421 tcacgtacta cacggctcac cgcatgctgg tcgatcgggc caaggttcag ccaggcgagg
     3481 atgtcctcat ctggggggcc ggcggcggcc tcggggtgtt tgccgtgcag ctctgcaaac
     3541 tgatgggcgc tcgcgcgatc gcagtcgtgt cgagtgacga caaggcggag ctcgtcatgc
     3601 agctcggagc gcatggtttc atcaaccgca aggacttccc ggcgcttcag tacaaggacg
     3661 gcatgacgcc ggaagaggaa aaggctcaca acgcagacct caagggcttc ggcaagcgca
     3721 tctgggagat cctaggtgag cgcaaaggcc ccgaggtggt gttcgagcac gttgggaaag
     3781 cgaccttccc tgcgagcgtg tttctcgcct cgaagtacgg gcgtatcgtc atctgcggtg
     3841 cgaccacggg ctacgacctc aacttcgacg tgaggcacct ctggatgcgg cagaaatcga
     3901 tcattggctc gcatttcgcc gacgccgatt ccgcaagtcg tgcgaatcgc ctggtcattc
     3961 aggacaaaat caagccggtc atgacgcaac tcttcacctg ggacgaaatc ccgaaggcac
     4021 accagctcat gtacgaaaac aagctacacg gcacggtcgc gtgtctcgtc ggcgcgcctc
     4081 ggccagggtt gaagaacctc gaggaaaccc tcgctgcgat cgcaaacggt taggagtagc
     4141 catgcaggaa gtcatcaacg ccatcgaggg cgtctacaag cgggcacacg acaacgtcaa
     4201 agcgctggtc tccgatggcg agcgcatcag cgccaagaag ctgaatcaac accagctggc
     4261 cgcgcacgca gtcgcgtacc tcgcaacgga gctcgaggca tgccggcagc tcgccgactg
     4321 ggccgaacga gtcggcggcg aatacgaagg caaggttgcg cgcgcttaca tcggagaggt
     4381 tgctcgctcg atcgtcgggg gcgtcgacct gggcccgtgc gaaagcatca gcctgagcga
     4441 ggttgggatc accgacggcg acttgcgaga aaccgtcctc aagccgaacg tggtcgcctt
     4501 cgcaaacgag cactcgaacg gccaagtcta catcgagctc gccacacatg cgcggaatca
     4561 cggcttccca aatccgggtt acggcgacga gatgctcgac gagattcgtt cgcagttctc
     4621 caagttcgtc gatgcggtgg tcatcccgcg cgcgcaggac atccaccgca acgacgtact
     4681 aattccgatg gacatcctcg atcagatgag cgagctgggc gtcttcgggc tcacgattcc
     4741 ggaggagtac gacggcctgg gcatgagcaa agtcgcgatg tgcgtggtca ccgaagagct
     4801 ttcgcgagga tacatcggcg tcggttcgct cggcacacgc tcggaaattg cggctgagct
     4861 gatcatgggc ggcggcacgg aggagcagaa gagaaagtgg ctcccgaagc tggcctcggg
     4921 cgaagttctc tcgaccgcag tgttcaccga gccgaacacc ggctctgacc tggcgcatct
     4981 cggcacgcgc gccgtcaagc agcaagacgg gagctacgtg atcaacggtc agaagacgtg
     5041 gatcacacac gcagttcggg ccgacttgat gacggtcctc gcgcgcacga atgcggacga
     5101 acccggctac aaaggcctct cgatgttcct cgccgaaaag acccgacgga ctggcgaaga
     5161 cggcgaagcg gagttcgtgg acgaaggcct aaccggtacg gagatcaagg tgctcggcta
     5221 ccgtggcatg aaggagtacg agctgagctt cgaggatttc gagctccctg cggactcgct
     5281 gctcggcggc gaagaaggcg ccggattcaa acaactcatg cgaaccttcg aaagcgcccg
     5341 cattcagacg gccgctcgag gagtgggcgt tgcccaggca gctctcgagg atgcgatcct
     5401 ctacgcgacc gaacgcgagc agtttggcgg ccccatcttc cagttccctc gtgtggcgcg
     5461 aaagatcggc cgcatgatct gtcgcatcca ggccggacgg caactcacgt acttcagcgc
     5521 ccgcgagaag gaccaggaga agcgctgtga cctcgaagct ggcatggcca agctcttggc
     5581 tacccgctcg gcttgggaat ctgcggatgc caacgtccaa atccacggcg gaaacggata
     5641 cgctgaggag tacacggcct cacgcctgct ggtggatgca cgcgtcctca gcatcttcga
     5701 aggcgccaac gagattcaag cgcacgtggt cgcgcgccgg ctcctcgaaa gctgacgggc
     5761 gcggccggac cggccatact gtgccgggat gagccttgat cgcgtcggcg agaggcgaag
     5821 gccgattcgc attgcctttt gcctggtgct tgcggtgcaa gtgctactgg ccgtctgggg
     5881 cgttgccgac caatggacgc gcggccacaa cggctggaac ggagccgcgt accagaatgc
     5941 tgcgcggaat accctgcgct gggatgttct cttcccgctt cagtacgaca cggcaaacgt
     6001 acctccgaaa ccggagcaac tctacacgca cgcaccgctt gccctgcacc tgcacaacgt
     6061 cgtgagcgtc aagatcttcg gcgaccgtga gctctcgatt cgtctcgtcg cggggttctg
     6121 gagcgtcgcc gcgctcatca tgctcttcgc cgtcgtacgg aggctctggg acgacgctca
     6181 cgccctcgcc gcctcggcgc tctacgtcgc acttcccatc aacgcgatct acatcaacat
     6241 ggccaaccac tcggctggtt tcatcttctg gtcgctgctc gcgctgtacc tctacgttca
     6301 gcgcccccgc ggcaagctct ggccctggcc gcgattcgtc ttcttcctcg tcgtgatcgc
     6361 gatcgcggcg acctgggact ggcccgccta ttacatcgcg ttttgtatcg ccctgcactg
     6421 ggcatacagc gtcaagaaga gctccgagcc attcgcgcag ctcctcgtat tctgcgtctg
     6481 gatcggtttc tggttcgtcg gccattttgc cttggtcgag ctgctgacgg gcaacttgga
     6541 cgagctcact ggcacgttca atgcacggcg cgcgctctcc tgggaccggt ttcgaacgca
     6601 cctcttggtg gtgccagagc tgatgttcac ggccccgatt ctcggaatct cagcggcctg
     6661 ggccgggacg tggctgctgc gcttggctcg gggtaccgcg cgcgcacggg acttggtgcc
     6721 gctcgcgttt ctgttcgctg ggctcgttca ctactggacc tttcgctgga gcgccatcgt
     6781 tcattcgtat tgggcctggc cgatgctccc tgccgtggca attgcggtca ccgcttcact
     6841 cttcacgatg gcccgatgga ttcgcgcacg ggccggtgca gcggccggtt ggagtgtact
     6901 gctgctgctg gtcccgctcg cgatccgaat cgttcaaatc gtgcccgagg cgcgatacgt
     6961 cggcggctcg atgtggttct tcactccggt ccgcggagcg gttgacactt acgactcggg
     7021 acggccggag ctgcggtttg cgacgatggt gaaagcctgg accgatcgag acacgggcgt
     7081 ccagcttcat accagcctca agccgcttcg tttggaaccc cgattcgaca tcaccctcga
     7141 tcgggtgact catcgctggt cgcagcaccc gcgcaaggag atcccaaacg gccagggggc
     7201 caaagcttgg gtcttcatcg ccgcggtcga tgccttctcc gacgaggagc gtgccgcttt
     7261 cgcgtcgagg catccatacc ggcagtacgg agattacttc atgctcgacc tccgcagcga
     7321 aggccgcgac atcgagatct ggggcctcac tccgcttccc ttcgatctgc gatggcggct
     7381 ccttcgaagc gcgttcgagc ctccaatccg cgcgagccgg cagatcgcat ccgagcaagc
     7441 gctcgatgaa aaggtggcag cgctcgatgc ggactagtcc atggacttcg cctctcgagc
     7501 tctgggcctc ggcctatctc ggatgcaccg cagtcttgct gctgggcctc cttccggagg
     7561 gcgaggtctc gttcgcgctg ctcagccagc tctgctggct tgccttgatc ggctcgatcc
     7621 cgaccggctt ggtcctaggg gccgcatcgc gcgtcgccac atcgtcgacc gccgtcgtct
     7681 tcgtctcgac gcttgcggtg atctcggttt cgagcctgat cgcctgggag cacacggact
     7741 tcttactctc ggggccatat tggctgcttc atcagcagcg cgaggtcgcg cgcatcggca
     7801 tgctcgcggc agtggccaca gcatgcggag gaggctggct ctggctgctc ctgggctcgc
     7861 gaatccaaga tcgcatcccg ctcacagcct ggtttgctct cacgctcacc gcagtcgcat
     7921 tgctcacctc cgcgatcgtg cgttaccgag cctacgacta ctcgatggcg cagctcgtgt
     7981 ttccggcggg tgttctcggc gccgccgtcg tgtaccgact gttccgtcga tcacggtatc
     8041 ggccgctcgt gctcggcgcg gcaatggcat gtctactctg cggcgcgggc tcgcgcttcg
     8101 atccatcgat ggtggccacg ggtcaacgtg aggtcatcgc gcaaagccga gcgggtgcgt
     8161 tgaccaccgt ctacgttttg ccgcagtttc gaaccgacga agactgggtg gtgaacggtg
     8221 tcgcctgccc ggatccgaga ccttcattgg aaacctcagc catcggtatc gcgcccgagc
     8281 gacgtcgaaa cgtcatcgtc atcacggtcg atgccctccg aaaggacgtc gtgggaggca
     8341 tcgtcaacgg gcgggccgtg accccagcgc tgtcgagctg ggcaagaacc gccgtgtcat
     8401 tcgagaacgc cacgtcgacc tacccggcga cgctgttcgc cgtaggcagc gcgtttacgg
     8461 gcttgagccc cgccgagctc tatctctctc cctcgcttcc agagacgatc ttcacccgga
     8521 cgcgggcgat catcgaccgg caagtggcca tcttgcccga cgtgagctgg tttcgccttc
     8581 cgatcgtcgg gcagtttctg gcgcctggag tcgagaccga gttcgcacct agcgatgcgg
     8641 cggcgaccga cgcgttgatc gaacggctga ggcaagcgcg gaatgacggc gcgacggtga
     8701 tggcctggct ccacttctac tctccgcatg atccgtacgt gccccaatcc gacttctcgt
     8761 tcggcaaggg caagcgcaat gcctacctga gcgaagtcgc atacttcgat cggcagctcg
     8821 gacgcttgat gaactaccta gaccaggaac attggctgga cgacaccctg gtcattttct
     8881 tctcagatca cggagaagcc cttggcgagc gctcgtactg gggccaccac gtctatttga
     8941 atgcatggat gatcgacgtg cccctcatgc tctggcattc ggacctagtg cccgccgcgc
     9001 cccttgtcgg tgtgagcctg gcagacgtcg cacctaccgt tcttcatttc ctcggattgc
     9061 ccctgccgag cgacatcgcc gctgagagcc tgttcacgct cgaccctaac caggtgggca
     9121 ggccgacgtt ctccgaagcc tttccggtcc gcggccgaca gctcttcgag agctttcggc
     9181 ttccggcgct cgatgactcc gcgattcgcg ccagacttcg cagcattcgt cagagcagcg
     9241 aaggctacga accaaagggc gcgatcaccc aggaccggta tcgcctgatt caccatcgag
     9301 gcgcagacgc gactctgttc tacgatcacg agctcgatcc cggcgagcgc tcgagccaag
     9361 gcgtcggaaa tcgggaacgt gccgagctcc ttcgaagcga gctcgagcgc tgggaacagg
     9421 agcagctcag gcgaattcgg tgcaggctcc agctcagcgc ggatccggct gcgacgtcga
     9481 atcctcgatg acgcgcacgg actccgagcg aatcgcggcc cggctcatcc cgagacgcgc
     9541 ttcattgaat cgaatgcgtg cggcctgtcc ggcgaccgta gtcgcataga gctgaaccac
     9601 cagttgaccg gagtcgtccg ggaccccgat ctcgtagcga agcttccaac gctcgccatt
     9661 tcgaaccagg aaacctttgc gctcgaactt ggagtagccg ttcaggaaaa catcttggtg
     9721 aaagtcgacc acggcttcat caccgagaac ttcgaggtcc agcaccactt cgaggttcgt
     9781 tcccgcggga acgcgtagat actggccgcc gatgagaagc ttctttccgt cggaggccct
     9841 cacggtgatt tcctcttggg cgagcagatc gagtgcttcg gtctgcgtca tgtctcgacg
     9901 gcccgagcgg gtcaggcgcg cgacctccgc caagagctgc cgctcacgcc catcggcagg
     9961 ctcgctccgt cgactcggtc cgaataccag gcttcgctcc ggaatggtcc gcgtacaatc
    10021 ttggaaggac tcgctgcaga cgacggcgct gccagatgcc tcccacatga tcacgcgacg
    10081 tgcgtaagtg tttcctgcga agagccgtcg gggcacggcg tagtcgcgaa accagcttcg
    10141 tcccaagaac tgccgcgcct caccttcgat tcctagaaga tcggtcacag aaagcgcagt
    10201 gtcgacgtgg gcctggagcg tgtcgattcg ttttcgctgc gggatcggca gcatcaccac
    10261 cacgaacgac cagctttgag agagcagcct ctgggttgcg ctctccgcgc gcgccaggcc
    10321 ggccgattca tccgaggtga tgatcacgac cgtgtcgcgg agcacgcctc gtcgttggag
    10381 ctcatcgagg aacacggcga gcgcgtcgtc ggcccagaga aaggcgcgct cctgcctgga
    10441 tcgtgcaccg tcaccgcccg catctgcggg tatcgtaaag ggatgatgcg tgcctaccgt
    10501 caggagcgtg gcgaagaacg gcgccgacgc ctcgtgaagc gcgaccactt tctcgatgga
    10561 ctgctcgaag aacgctttgt cgtcgacgcc ccaatcggtg cgggcatagc tcttctcgaa
    10621 ccagggatct ccgacgagct ggtcgaagcc cgctttcttc atgaactggt ccttgagcat
    10681 gaatccgagc ggggctgctt gtatgtacgc ggtgctgtat ccgagcttgc gcaagacttg
    10741 gggcaggcac aggcgcgccg agccgtaggc ctgctcgctc atcttggatt ggtcggtgag
    10801 gagcttgggg tagtccccgc atagaattcc gtactctccg cggttggttt gcctttgatg
    10861 ggaaatcact tgcgtgaaga cgatgtgctc ctgcgccagt gcgtccagcc tgggcatccg
    10921 cgtcgtgctc tcgacgcctt gggcggccgc gaccgagggg aggtacgcgc ccgaagctcc
    10981 ttcgaccatg atcagcagga cgttcggccg atcgagaaat ggaccgatcc agcgctcgcc
    11041 atgcagattg cttcgaaaga cgtctcgtac ttcggcgctg atcgttcctg attcttcgtt
    11101 cggagaggcc ccgacgatcg ctaggttggc gtagaccgcg tgacgctgac gccactcgtc
    11161 ttgctgatgt gaccaaggaa tggctacctg gccgagcacg ctcgtggcga acgcgagccc
    11221 ccaccagcgc caccaacgct ctttcgggcg acgtgccgag aacgccgcga aaatcgcgag
    11281 aaccaggaat gagccaaaaa caaacggatg cgccagacga cccgctgaac cacctaggaa
    11341 gaccgaatcg gtcaaaaagc ctgcatggct gagcgcataa agagaatcga ataccgagat
    11401 gaactcgtac atggcgaaac tcagcaccac gaacgcaatg aggaccagca gtccgagcgc
    11461 tctgccccag tagcggccga gggagaagag caggcctaca gcgcccatca cgagcaaggc
    11521 gatggacgtg tcggagagaa accctcggag atcggccagg gcgaggagca tccccttttc
    11581 gcgcaagagc aagccacgcg tgagccaagg cgcgccgagg caaagcgcca cgaccagccc
    11641 ccaacctgcg cgtccattcc aatccggtgt gtctgcttcg atgaccggaa cttatgagct
    11701 atgtcgatac gatggcaaag ggattcgcgc ctgcggccga gcgcaatcgg caaccgatcc
    11761 tcgacgtgct tcggcgagtg ctcccgcccc gaggcctggt tctcgaagtc gcgagtggaa
    11821 caggtcagca cgcggtcttc ttctcggagc agcttcgagc ccttcgatgg caaccgagcg
    11881 acgtctcgcc cgatgccctg cggagcatcg gagcctgggt cgccgagctt gaccggagca
    11941 acctcctcgc gccgttcgag ctcgatgtgc gctggccggc atggcctctc gcagaagccg
    12001 atgccgtgct cagcatcaac atgatccaca tctcggaatg ggaaaccagc gaggccttgt
    12061 tcgaaggcgc gcgcaagctg ctcgcgcctg gcgctccact gattacctac ggcccttatc
    12121 ggcttcatgg cgaacacacg gcaccgagca acgcagcgtt cgacgagagc cttcgatcgc
    12181 gcaacccgcg ttggggcgtc cgcgacatcg atgagctgac caagctcgcc gaccggacgg
    12241 ggtttacact ggaggagcgc gttcagatgc cggcgaacaa catgacgttg gtatggacgc
    12301 gcgacgccta ggacggcatg tgcggcagcg acgggttcca gaagagcatc acgaaataca
    12361 agacgaagac gagaatcaag gcggcgcccc gcttgcgacc gatgtggctc cgctcgttga
    12421 ccgccatcaa gaggagtaga agggtcgcac ctaccatgat cgggaagtct cggaagatga
    12481 cggagcggtc gacttcgccg ggagcaatga tcccgggaag cccgagcacg ccgagcaagt
    12541 tgaacatgtt cgagccgacg acattgccca ccgcaatgtc cggttctccc cggcgggccg
    12601 cggcaaccga agcagccagc tcaggaaggc tcgtcccgag cgccacgaga gtgagaccga
    12661 ccacgagctc gctgacgcca aggtcgcggg cgattccgac agcgccccag acgaggacgc
    12721 gagatccggc caagaggacg agaaggccga ccgaaaggac ggcgacggcc ctcggtgtag
    12781 ggagcgagct cgggatctga tcgtcttcga aggccaacgc tggatcgctg atgtctcggg
    12841 accgctcttc acggccttgc aagctaatcc aaacgatcaa agcgaacata cccgcgaaca
    12901 agacgaaacc ttcgacccgg ctcagctctc cgtcccagag aaaggcccag gcgagcccca
    12961 tgatcaggaa caagaccgga atctcgcgaa tcaacagcct cgagtgaaca ctgagcggct
    13021 gcgcgaatgc cgctgcgccc agcacgagcg tggcgttggt gatgttcgag ccaatcgcgt
    13081 tgccgatgcc catctcgcga cttccggcgg ccgcggccat gcccgaaacc aacatctccg
    13141 gcgcggaggt gccaaaagca acgatcgtga gaccgatgac gaggcgactc agtccaaggt
    13201 tgctggccaa acccgccgct ccatgaacga aacgatccgc tccccagacc accgcgatga
    13261 aacccacgat gacagaggtg atggcgtaga acagcgtcag aacgacccct cgagcgaaac
    13321 gggcaaagcc tcagttttgc tcagtcgtgg acctgcgcaa cccccgtgca gatggcactg
    13381 gtaaaaccac cctgctgagc gctatacgtc tctgagcaaa ggaggggacg gcatggctga
    13441 gcgcctagag ggcaaggtgg cgatcgtaac gggggcaagc aggggaatcg gtgcggcgat
    13501 cgcgacccag atggccgagg aaggagccaa agtcgtcctt gtctcccgca agatcgaagg
    13561 cctcgagacc gttgcgggtg aaattcgcga caagggaggc gatgcgacgg cgatcgcctg
    13621 tcacgtgggt caccgcgacc agcgcgagga catgctcgag aaggcgctga aagcctacgg
    13681 caaggtcgac atcctcgtga acaatgccgc gaccaaccca cacttcggcc caatgctgac
    13741 aatcgacgga ggcgcctggg acaagacctt cgaggtcaac gtgaagggct acttcggaat
    13801 gatccagctg gtagctgggc acctgcagca acgaaaagcg cgggggtcgg tggtcaacgt
    13861 cgccagtgtc gtgggcctca tggcggcccc gatgcaaggc gtctacgcga tgaccaaggc
    13921 cgcggtcatc tcgatgacga agaccctggc gatggagctc gggggtgcag gcattcgggt
    13981 caatgcaatc gcgccaggcc tgatcgaaac gaagttcgct caagtcttgg tggacaacga
    14041 cgagattcgt tcgagcatcg tcaatcgaac cgcggcaggc cgtgtgggtc agccgcggga
    14101 catcgccggc ggtgcagtct tcttggcgtc ggacgaatcg gactacgtga cgggggatgt
    14161 catggtcatc gacggcggct ggacactggc gtaaccgggg cggccgatga tgggtaagcc
    14221 gccaccgagc ccgggagagg ttgcgcgtaa gcgagacctg gtcgagtctc gcatcgtcaa
    14281 cctgcgccgt gagctcgaat ccgaaaccga gcccaggaca caggccgcga tactctacga
    14341 agtggcggcg ctccacgagc acgagctcga agaaatttcc gaggcgttca gctgctacgg
    14401 cgaggcgtac gcctcggctc cgagctttca gccagccttg atcgcccagc ttcggctcgc
    14461 tgaacggggg gacataggct gcgattcgag cgccatcacc tcgtcgctcg ccgcacatgc
    14521 tacgagcccc tccgtcagcg ccggggcctt tttggatctc gctctccggt ccgaggactg
    14581 ggcatctcat ctgcgcgagg ccatcgcgcg ttctccggag cctgcggtgc ccgccctcat
    14641 catggaatgg ctggccgaag cgcgcggaga ccaggattcc cttcgactcg cggttcatac
    14701 acaggctcta catgcatccg aaccaagcct tgcggctgct ctcttcgtcg acctcgcatt
    14761 gagcgaaatc gaagctgggc gtcccgaaga ggcgatagag gcactcgagc acgcttgcga
    14821 agcccgcgcg ctgctctggc aagcacgctc gcttcagctt cgaatcgcta gagagcacgg
    14881 gcgctgggac gtgctgatcc gcgcggcaac ttcgatggca cgcctgttag aggcagcaag
    14941 cgaaagcgac gaaccgaccg atccactcga gctgccggtt cctccggtgg aacgacttcc
    15001 gatggccgcg ttcctgtggc aagaggcttc ggcctgcagc gacgtgcgac tcgacgacgc
    15061 agactcagcc gcgcaataca tcgaagcggc tctgcgcttg tttccggatc ggtcgtcgat
    15121 tcgactgaaa tccttgcgac tcgcggacaa gcggggcgat gatctcgcgt tccagtctgc
    15181 ctgcgactgg tttcgggcga acgcgcccgg cgatccggca ttcgtagcgc aggggcttcg
    15241 ccgtgcgctc gctgaaggag atggttcgca gacccaagcg atcgctcaaa acgctgcaac
    15301 cgtgtttccc caatccgaat acctgcaggc ggcgcgcgac gtggccgcga ttcggaccgg
    15361 ttccgtcgca gatcgggtcg agcacttgca ggagtgtgcg gaatccgcgg aaggagaagc
    15421 acgcgcccga tgcctgtggc acgccgccca gctcgccaat tccagcccag gaaccgagcc
    15481 ggatgcgcag gtcctttaca gcaaggcgat cgcggcggcc ggcggctcca agacccggat
    15541 cgttcgagaa gccttgggtg ccgcgctccg cgcgaagcaa cccgatcgaa ttctcgagcg
    15601 ttgcgccgag ctgagccaat gcgaagtcga ccccgctgag cgttcgtcga tcggattcac
    15661 ggagtactac gtgacccagc atggctcgag cgccgacgaa ccagcccagg tcgtgctgcg
    15721 cgatgcggtc cagaacccag acagccgcga atgggccccc ggacttgctc gggcccatgc
    15781 cgcatggtcc ggcaacgagg ccttgctggc cgaggcccac gaagcactgg caggagccac
    15841 cgacggcgaa tcgaagctgg ggcacctgag cgccgctggg caggcccatg ctcgaagcgg
    15901 gaactggagc gcggccgagc gcgtacttcg gcaagcctct gccgcggcgc cagatgatga
    15961 ttacgtccta tccctgctcg acggggtatt gcgggaggga gggcaccccg aagacgtggt
    16021 gtcgctagca agggagcgcg tgcaaggccc ctcggggacc cagcttggtg aggtgtcact
    16081 tcttctggct ggcgcggccg cggagcgatc cgagaacctt catgccgccg agcaggctta
    16141 tgagcaggct gtcttggaag accccagctc gccctccccg gccttggcgc ttctcgatat
    16201 cgctcgccgc agaggggacg cagaggcgac cctgcgcgcc tacgaacggc tctcggagtc
    16261 caatctcggc ggcggcgtac cggagctcta cgcgctgctt cggggcgacg cgctcgcgtt
    16321 ccgggaaacg gtcggcgcag agacgagcga cgcgtacgaa cgaggcctcg agcatccgac
    16381 cagcgcggtt gctgccgccg ttgccctgat gtccttgccg cgtcggctca cgtcggcaga
    16441 gcagagggcg gcctcggagg aagtccttgc ggacgtcggt gaatcttctg agaatatcgc
    16501 tgaagagttt gagaccgcct acggcgcact ccgtgcgtcg ctcgctgaag agagctcgtc
    16561 atccggcgac gcctggcttc agctcgcggc tctggcgcca agcgagagcc tccgcgccgc
    16621 ggcgctgctc caagggcttc gggcgacacg aatcgcgcaa ggagccggtg cggcagacga
    16681 gatcttcatg ctggcgcaag atgccggggt gctctccgag tcgcaaagcg acgctacgat
    16741 cgcgatcgac gaggcgctcg cgcctggaga tgacgccgag attcgtgcgc acgcactcga
    16801 acacaagaga cgacatagcg aagcgattgg acgcggcgca ctcgacgcgg cgtattgccg
    16861 tgctctggtc gaggcacacc ggggggcgga agccgtggcc ttgctgagcg ctgccgtgaa
    16921 cgagcggccg gacgacctct ccgtctggga gatgctccga gacgcagcgc ggcaagcggg
    16981 cgattggccc tcggtcgcgc aggcatgcga gcgccttgcg cctttcgtcg acggttcgct
    17041 caaggccgat ctcttggagg aggccggcgt cgtgcgattg gatcacctcg agcaatacca
    17101 gcaagcccgg gacctatttc gcgccgctct ccacgaagac ccgcgccgcg atgttgcgtt
    17161 caggcgattg cacgacctgc tgacagcgca ggaggatgcc gaagcgctcg aaacgctcgt
    17221 gtcggagcgg ctcgcgctca gcgatccgga agaccgtcaa gagctcttgt acgagcgtgc
    17281 ccgacttctc cgcggcttca gcgaccggcc tggcgcactc gaagtgctgg acgagctcat
    17341 cgcctcggaa cccgaccatc caggggctct ggccttggcc gccgaaatcc acgtctcgct
    17401 cgagcaatgg gcggaggccg tcgcttgcct ccgaagcttg gcgatggccg gtattccaga
    17461 ggaacagcga cgtgtggcgc acctcggcgc tgcagacttc ctcgaaacgc acctcggagc
    17521 gaagtccgaa gcgctccagg agctgcgcgc gatcgaagcc ctcgacctcg ccgacgccca
    17581 gatttggacg cgcatcggcg ccctcgagct cgagctcgag aaccgcggag ccgcaatcga
    17641 cgcctaccag cgggctctcg atgcggaacc cacgaatgcc gccgcgatct cgattctcat
    17701 cgagctactc gacggcgctg cgaggaacga tgcgctcgtg gcgttcgagc aggccgtctg
    17761 gcaacggatc gacgagggtc gcctcgatgc cgcgcttctc gaaggattgg ccgacgccgc
    17821 gagctggcga ggagacgctc gacgaaccgc cgcggttcac gccgcgcggg tcgcgctggg
    17881 gctttcgccg agctcgggcg tcagcaactt cgacctcgac caagtctccg tagccgcgct
    17941 ctgggatgtc aatgctgacg agttgcttcg agacgtgctc cgtagcgcag gtcccgcgct
    18001 gacgaagtca cgatggcgaa cgaagagagc ggcgccagac gatccgattt gcacggagct
    18061 cgagcgcatc tgtcagcgat tcggcacacg cgcaggcttg gtcaagcttt ccgatgaacc
    18121 cgatgcaacg ctcgaggccg agctaggcga ctcggcggtg gactgggtcg ttccgcgcgg
    18181 atcgaaagca ggcctcgacg ccgcaggccg attcgcagcc ggccgcctcg cttggggaac
    18241 gccgcacggc gctgcgcgac tgctcgatga ttccccggag aaagttgcag gcaagctcgc
    18301 tgccattctt cgagccgcgc gttgcgagct cgcccccggg ggacctcagc taccggcagc
    18361 tcagatcaag ctgcggcgaa tcgtccgcaa ggcggtcaag gaccgagtgg gagacgcgac
    18421 gctcacagct ccggcgttga tcacgtttgc gcgaagtctg cagcgaagcg cggaccgtgc
    18481 gggattgctg acgtcgggaa acatcgcggt ggctttgagc aagcttctcg gcgggaaccc
    18541 ctcgctgaac gcactgcgca gctccccgcg cgggctcgac attctcagat tctggattca
    18601 cgccgactct ccgctctggg ggaacgatgt ctgaagacga cctcagcgct gcaagccggg
    18661 agtcggaagc gccttcattc gactttgacg aggatgcgat cgcgaccctg ctcgacacct
    18721 tcgtggagac acgaacggga cctcaagcga agcgggtcag ccgccccccc gacccctcgg
    18781 attcgggggc ccaggcggca cagcgaaccg tatttcaggt gcgctcccgg gacgagagtt
    18841 tgccgctggt cggtgagcgc cccgaagcca agaagcgacg catcgaactg ctcgaagcgc
    18901 tcgcgggccg cgcggtcggt tcggcgaaag cgagactact cacgtctgca gctgagctcc
    18961 acgagcggct tggcaacgcc gaggccgcgg aacgtgacta ccaaatggcc ttgggtgcag
    19021 atgcacgcga cgtggtcgtc gtgcgtgcga tgaggcgatg cgcgatgaac cgcgaggact
    19081 ggccggccgc ggccgacgcc ctcgaaaagg aagcgggcct cgagctgagc agctcggaac
    19141 gaggctccgc actcaagctg ctcgcgagca ttcagctctg gaagctccgc gatccagccg
    19201 ctggggagca ggcggcttcg catgctgtag cgcttcggga tgaagacttc tcggcgctgg
    19261 tcttgctagc atccgcgcga atggcgcgtg gcgacgccgg aggtgcagcc gacgccctca
    19321 gctctgcggc tgaacattgg ccgcaatccg aagctcgcgg tctcattcac ttgcacgctg
    19381 cggagctcat ggaggatgcc ggagccctcg aatccgctca acgcgcgtat cagcgcgttc
    19441 tcgagctcag tccgtcgcga cttgcggcgc acctcggcgt agtccgggtg tctagagcgc
    19501 tcggagcctg ggaaccggcg atagaagcgc tgcaccaagc ggccgaagtc gcccccgcgc
    19561 cagtcgccgc ggcactcctc cgcacggctg cgtgtgtgcg gatgaggacc gggggtccga
    19621 aagaactggt cacactcctc gagggggcgg tcgacgtggc ttcgcgatgg acgcttgctg
    19681 aagccgctgc actcgcaaac gagctcgaac gggcgatcga ggcgtttgac tccgacgtgc
    19741 cgggcctgag cccagaagct cgcactatga gcagcgcccg ccgcgcgcgc tttcacgccg
    19801 agctgcaaga ccctgcacga ttcgagcgcg ccgcgagcga gagcggatcc gcgcgcagct
    19861 tgacccctta cctccgggct tgccaccgtc tgctgagctc gaacgaagag gacgaaggca
    19921 gactgcggag aatgctcgaa accgtctccg acgaggcatc gagcccttcg tcgcgcatgg
    19981 ttcgcgcgga cgacgctgcg agggatggga acggtgcccg gttcttccag gccctcgaac
    20041 acgagctcga tgaggctcca gaccatctcg ctgctggcgc agcgctcgca tgggtggaga
    20101 tggccgaaca ctccgcccgg atcgatcacc ggagcgctct gcttcgggca gaggagcgca
    20161 tgccgcagaa tccgctgatc gcgcaagccc tccttctcga cgacgatgat gtgtccgcga
    20221 acgccagacg ctggtccaac gcgggccggg ccacagaggg cgatcgacgg gcatttgcgt
    20281 tcacgatggc cgcgcgactc gcttcacctg aaaccgaggc ggcgatgggt gcttctgacg
    20341 aggcgctggc ggcgtcgacc gactactggc ccacgctttg ggagctcgag gaccagattg
    20401 gcacccccga tgcgcgcgca gcaagcgctg tcaggcaagc cgagctcgac gcggcgaacg
    20461 aaattccaga catgctgcgg gcatcgatgt ggacgacctc ggcgagcaaa cggctggagc
    20521 ttgccgcggc gacgctcgac cgggccgcgc ccgatccact cctgctcgaa catctcttcg
    20581 acgccgccgg gactagcagt gaggctgcag gcgatctgat gatgctggct gcggatcgaa
    20641 tggccgggct accatacttc gaacgcgccg cagactcata ccgcttggcg ggccttccga
    20701 gccaggcagc caaggtgctc cgcgcggcat cggctaagcg gcccgaagac cccatgatcc
    20761 gagtgcagcg aaaggacgcc gagctcgaag cctgcgagtt cgcgcgtctg gccgactcgg
    20821 caatgcgccg cgcacgcgag gcacggaccg acgaagagga gctcggcgcg tttgctgaca
    20881 tggccgaggt cgaccgactt gcccgccgcg acatgcaaag cgcgcgtctg gcgctgcaat
    20941 cgatcgccga atcgcgcccc gatcacatcc ccaccgcccg cgctctcgaa tgggacgcgc
    21001 tgcgggaaaa cgacaccgag cgcatccgct cgagcgctcg ccgcctcttg gagatcttgc
    21061 cagcggactc ggccgagagg ctcgctaggc agaggctcat cctcgaatca ctgagctcgg
    21121 atccggacat catgcggatc gacctcgacc ggcagctcgg ccggatcgag gagccgctcg
    21181 cggcggaccc gggcctcgcg agacaggtgc tcggcgcagc ctatgcaggc agccagccct
    21241 tgcgttgcgt tcccgcactg gaagccttgc ggagcaccct cggtgacgag ctcgaagagg
    21301 gggcggttgc cctggacacc gcccgcgtgc tcgagcgcat cggggatcac gagcgagcgc
    21361 ttcaaacact ggacgcagct ggaagtcatc cgctcgcccg ggagtcggaa gcacgacttc
    21421 tgcacacggc caagcgctgg gaggaagccg cggccacata cagagacgct gcaacccggg
    21481 ccaaagacag ccatcgtgcg gcggccctgt ggcgcgaggc agcttgcatc ttggaagaag
    21541 agctcggcaa cctcgacgct gcagtcgatg cctgggttgc cgccgccaac tgcgacatcc
    21601 ggtatctcga cgtgtaccgt cggctcgctg ccttgtaccg aagacagggc gagctcgatc
    21661 ggctcgccag cctgaccgat cgacgaatcg atgcgggggc cgatactccg accctcgtga
    21721 gccttctcgt cgacaaggct cgacagcgtc gtgagcgtgg cgacttggaa ggcgtaattg
    21781 ccgctctcga cgagtgcctc gagctcgatc cacaccactt cgccgcgctc aaagagctgg
    21841 tcgacacgca tcgcggagct gaagattggc aaggggctgc ggaagcgctg attcggatcg
    21901 cgcgattgaa gcgcagcgtc gaggagcaga gctgggcgtt cactcaactg gccgagattt
    21961 acgacgtgca ccttcacgat ctttcgcgcg ccgaagcctc cctacgccag gtcgccaagc
    22021 tcgcgccaac gcacttggaa acgctggacc gcctggcgtc ggtactgagc cgggagaaca
    22081 aggctctgga agcggcccgc ttgctcgaag agctcatccg gcggacgact ggcgaaaccg
    22141 agctgcgcga ctaccgcatt cgcttggctg tcgcagtgga atcggccgga cagccgaggc
    22201 aggccgagct cttgctcgag agcctgcgtg cggagcagcc gacggagatc gacgtgattc
    22261 tcgcggcagc agaccactat cagcgccaag gcgcggcccc ggccgaagcc atgcacttga
    22321 atcgggccgt cacagacctg cggtccgcga tcgacgccga ccccggcagc gaggctctat
    22381 ggacgacact cgttcgtgtt cttcacaggc ggcacggtcc cggcccggcc tcttgcgcgg
    22441 ctagcgccgc cattgcactc gggcacccgg catcgctgtt cgacggcgac gtcagcaggc
    22501 aaaacgaggc cctgggagag ccgaaggttc cgatgtccaa gttggtagac agcatcgttg
    22561 cgccagtcgg tttgccgcaa accctgcggc gcctcttcgc gctcagcgaa cagtccttcg
    22621 acaaggcgct gcctttcgac gcgagcgcct ggcacctgcg acgcccgtca gggcccttca
    22681 tgagcctcgt ggaggaggcg ggtgcggtcg ccgaggccct cggaatcagc gagccccgcc
    22741 tccgggtcac gcacgtcgcc cccgcggcct gcatgccggt ctccggcgac ccaccgacga
    22801 tcgtcgtggg cgggaagctc catgaaatca ccagtgcgcg cgaaagggtc tttctgttcg
    22861 cacgcgcatt gaaggtcgcg agcaatcatc ttgcgccagc ccttcgcgcg cgcccggagg
    22921 agctcgacgt ggccttgctc gcattgctcc aagcccacga tgcgagccgt ggcgagcacc
    22981 cacgtgcccg tgagctccaa gaccttcgca agaagctcct caagtccgtg ccgagacgct
    23041 ggcgcgacga agtcgagagc ctcgtgctcg agttacgagg gaacgcgagc ttttcgacgc
    23101 gctccgttcc gatggcgatc tcggagctgg gcgaccgagc ggccttgacc ctgaccgggg
    23161 acgtcccgtc ggcggtcgat gcgctgctca agatcgccgg gcacgatgtg ccggccagcg
    23221 actcaggccg attgggggct attctcgaaa accgcgaagc ctgggcgatg gtccgattcg
    23281 cgatcgctga cgcacatttt gaagctcgcg cccaagctgg tgtagatccc tagaacgatg
    23341 cgattgggtt tcatcggtcc agcgaaaacg gacacggctg ctctcgagcg cgccgccaag
    23401 cttctcatct gcgacctcga ggtcgacacg ttgatatacc tcggtgaaga cgatgctctt
    23461 cgagagttca tcgccgcgca cacgaacggt ggcagcgagg aaccgatcga gcagcaggtc
    23521 gccgaggttg ctgcaaccgg ctctcccgaa caaatcacgg aggtactcag acggcttcgg
    23581 ggcgcacgct acctcggaaa gcttcgcgtc gtgcctgcgc ctccaattcg cgcaatggag
    23641 atgctggacg accggatcgt gctcatcgtg agaaacaagt cgacgatcgg agaagaagac
    23701 gtgatcaact caaacctcgt cgtgtacggc gacggaaacg agttgatgtt caagcgcttc
    23761 ggaccccgtt gtttcttcag ccccggtcct ctcgatcatg gacacgtggg tgtgatcgat
    23821 gaccaatcag agaccggtgg tgtcgtcctc gaagccatcg acgtgaacgg cgatctccgt
    23881 tggagagagc caatccaagg gcgcggagca aaggtcatgg tggcgccgtg accgccgagc
    23941 ggatcgcaat ctatggaggc agcttcgatc cgccgcatct cggacacgtg ctctcggtcg
    24001 cttgggcact ctcggccgcc gagatcgact ccgtctgggt cattccaact tggaaacacg
    24061 cgttcgataa ggagcatcgc gcctcgttcg aagaacgact ggctctggcg gagctcgcat
    24121 tcgctccatt ccaccaggtt ctcgtctccg acgtcgagcg gcgtctagga ggggtgagcc
    24181 gaacgctcgt cacgctcgag gcactccaag cggagcgccc aggcgccgta tttcgccttc
    24241 tgatcggtgc cgatgtcttg ccgacgacct cgcgctggca ccgctgggaa gaggttgccc
    24301 gcctcgcccc gccgctggtc gtcgggcgtg aaggataccc tgctccagag ggctgcccca
    24361 tctcgattcc gaacgtcaac tcgaccgaga ttcgtctccg gctctcgaat gcaggcgaga
    24421 tacgcggcct cgtcccgacg aaggtggtcg agcacatccg atcccacggg ctctatcaag
    24481 gctctccatg aagctcctga tcatcggccg cggccgggtt ggcaa
//
LOCUS       DASRZF010000304        10522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4360742, whole genome shotgun sequence.
ACCESSION   DASRZF010000304 DASRZF010000000
VERSION     DASRZF010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 10522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10522
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4360742"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            607..1065
                     /locus_tag="VFG22_13230"
     CDS             607..1065
                     /locus_tag="VFG22_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417252.1"
                     /translation="MSFVITSDFDFTGDNVIVAYSTNEYVIQIVDEAGDPFDLTPYEE
                     VRAEFRNHSNEDQLPGSQASDVVYDADYSATNITPETHTGFVSPKTSGQIRVVIGAVE
                     TKLEQDGSARKSGLWDAVAYTTATGADESFQTKVAKGAWSTQWTSTLQTP"
     gene            1069..2748
                     /locus_tag="VFG22_13235"
     CDS             1069..2748
                     /locus_tag="VFG22_13235"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET6417253.1"
                     /translation="MANISTVSSPTGLRSRGKITTDPPNILVILADDVGIDMLAQYQA
                     YMIANAGFPSPAVSYADTPTLQSMKTAGVRFMNMRTQPNCSPTRACMLSGRYAFRTGI
                     SAVVREANMDLTANAWEFGINHATDELFMPRLTIPKGYRCGIFGKWHLAAPDGGTVPN
                     GVPWEGGTPAGYGTGWTHPDTISSTDGQHIDYGLTFSNLDIEPLPADSGSTKPGYHNF
                     YWRSKTEGNDATTTQVADEYHTAYSRQELQSWIQGQTEPWLAYWALCAIHSPFGTFPD
                     NGGGNSGCMPPDDGTTATDWDTYNDTIGTYPVYNSCRAAIEAMDYQIGLLKTALGDEW
                     DRTMVLFIGDNGTQRNVLVAMQDWETANANAIGEPYASLLSEDAFKGTVHEAGLRVPM
                     MVTGPLVASPNRDYLGLVDAVDVYATIRHIARVPYSSTIQDDRVIDGISFLHALQDGT
                     LDVEGSRQFSFSETYIPLGAATGATDTDAGHQRDRSYILHVEGDGVYKLNRELSGTVN
                     VDTFFQLMDDDYVPVDPYMQTPLATGAGVPNYVACAQAMSALFATETESIV"
     gene            2751..3002
                     /locus_tag="VFG22_13240"
     CDS             2751..3002
                     /locus_tag="VFG22_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417254.1"
                     /translation="MARTISATAVANGISYRLVRDTDTAGANQYWFESLSTDMALGQK
                     WSQLNTSSVSTAAGANALIPTGIPSAALAAIFEKLVAGR"
     gene            3753..5126
                     /locus_tag="VFG22_13245"
     CDS             3753..5126
                     /locus_tag="VFG22_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417255.1"
                     /translation="MVSMTQAPPKRVDQLRYHPVQSRLRKSNARFKVVEAGRRSGKTE
                     HAKRDGIQRAICAHMDPGIDDYRVAFCAPTRDQAKQIYWDDLKRMTPPEFVVSISETE
                     LSIHLVNGAMIAVVGLDKPARIEGRPWDDIKWDEVADCKPGTWDRHLRPALDTDGRPG
                     SAWIYGVPRPSSEFKRLADLGKDENEPDYEYFTWKSADILAPKVIAAAKRTMDPLMFA
                     QEYEASRVNLQGLAYYQFNRERNLRELQPDTRAPLIFCFDFNVQPGVSAVAQEFDSRE
                     LGKPPDGCQWASDSVTGWVGEAYIPRHSNTHRVCNALIESWGDHEGLVYGYGDATGGG
                     RSTVQGKEQWGSDWAIINDLMGRHFGDRWRDYHRRSNPRERARVNAVNARMCSADNTV
                     GTVIDPTRAPRLIEDLELTMILEGSAGELDKKSDPAATHLTDGAGYYIEQEFPAVSRD
                     IDSEPFN"
     gene            5126..6589
                     /locus_tag="VFG22_13250"
     CDS             5126..6589
                     /locus_tag="VFG22_13250"
                     /inference="COORDINATES: protein motif:HMM:NF024661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4055 domain-containing protein"
                     /protein_id="HET6417256.1"
                     /translation="MAIASDTVDTPSAVWEELYKRWELPLALRKGTDGMRAGGVKWLD
                     REEAETQGQYAARLKRTFLHPAFDDTVERVVAKPFSKPITFRGELPERLMPMLEDVDG
                     EGNNLDQFASELMLSAVTTGMSHVLVDFPRVDSPVSLAEERDMRLRPRFVHVPAERLF
                     AWSWDRMPNGGIQLSSIRYKETREVEVGAWGSELRNLIHVYRFNHDGGSTYQAWEQQS
                     DRITEGSYVPVEDPVPITFSGVPLVTYYTGRTGAMEAAPPLQGIADLNLAHYQVSSEH
                     RNVLNVVGVAMLFRTGVDGKAKDAKTVISPRAVYTSSVPDARMEWVEHSGAGVEAIER
                     YLKQTEDRMEVLGMQPFNSGTRLTATQRVMDESKTETTVQTWVRGLEDALTEAFRKAG
                     EWIGEEVSEDFAVQVYNDFSVGPRVESDLRALDSARARRDIDKRTFLEELQRRGTLDQ
                     DRDLDEIIEATMDEGPDMSALAGVVSALGADETEEAA"
     gene            6713..7228
                     /locus_tag="VFG22_13255"
     CDS             6713..7228
                     /locus_tag="VFG22_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417257.1"
                     /translation="MASYRPEIFIHIPKTGGTSICNSLRVEGKHTPLSHRPEGGFRFT
                     FIRNPWDHARSWWHHCQYGKRTSFEGWALDGCPGTWTVGDTGVHMPRQGDWFERLGDP
                     PMDFIGRFESLDADFGRLCVLIGVEGITLSHDNDRGGSKRPHYREVWTPAMRNATPWL
                     DEFAGRYGYEF"
     gene            7450..9279
                     /locus_tag="VFG22_13260"
     CDS             7450..9279
                     /locus_tag="VFG22_13260"
                     /inference="COORDINATES: protein motif:HMM:NF018154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HET6417258.1"
                     /translation="MTDPRLLDPAKTANEILQDRAIRHAIYIERLKVGEVRSTLAELN
                     ALYDELVARTFKVLDRIATRGAKVREFKSIRNLTRDLNRIAVDQYDSVARELMGRMEA
                     LAAAESRWQLAAITESATVPLTLKAPSRAILRAIVHNEPFGDPTRAVVRTLRGWFEAE
                     GEMSAFRIQSALDVGLRQGDTVARITRRVRDTFGVSGRNAETWVRTGINNTVTLARER
                     TYEENAEVVKGVQLLATLDSRTTVICMGEDGNVYPISEGPRPPLHPRCRTTTLPVLKS
                     WKELGIPLKELPEGTRASMNGQVPESVTYGEWLKTQSREFQDDVLGPKRAELFRTGAV
                     PIDRFTDERLRPLTLSQIVEREKLSPLTAGAFNPKAPETIGQYRAPNGVWAPERQKLH
                     DEIVREHLAGLPSQKNPTFHMMGGGPASGKSTVIKTGDVNLPPGNVLIDSDAIKAKLP
                     EYRAMQAAKAPGAAAFAHEESSYLSKRILAEAGDRRINAVLDGTGNGSTAGIFKKAGG
                     MRERGYRVVADYVTVDTETALARNLARAAKTGRMVPASYVSGVHETISRILPDAIEGG
                     AFDDFRLWDTNASGPRLVANLEGGRLKIFDERLWLRFLQKAGG"
     gene            9281..9496
                     /locus_tag="VFG22_13265"
     CDS             9281..9496
                     /locus_tag="VFG22_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417259.1"
                     /translation="MLTAEDMARIVKEETLGIDAPTNETQEAAAFRVKTAASVAEVHS
                     LGYEVEIPQEWPDISDFPTKEPRTGGA"
     gene            complement(9519..10274)
                     /locus_tag="VFG22_13270"
     CDS             complement(9519..10274)
                     /locus_tag="VFG22_13270"
                     /inference="COORDINATES: protein motif:HMM:NF023505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HET6417260.1"
                     /translation="MTRRERLERKLEKREDWATTRDRKSDAAFAASRKLANSIPLGQP
                     ILVGHHSEKRARRDVERIRSGMSAGVEHASMADHHRSKAGGLSAQLEGSIFSDDPDAL
                     DALAERVAELEAKRDNMKRLNASYRKCKRDLGAFCEAEGFDAEKRQRLEEARAMWPYS
                     KAWVPFESYQLSNLGANIRRLKSRAVDVANRQRRAEAASEAGGTLIEGGDWVRVTFEE
                     KPARATLDALKAAGFRWSGGSWCGMRESLPAGL"
     gene            complement(10271..10477)
                     /locus_tag="VFG22_13275"
     CDS             complement(10271..10477)
                     /locus_tag="VFG22_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417261.1"
                     /translation="MPKKQTFPELTDEEAETVRAYAEANGAEWKARLRSAWMRSAEPG
                     ILQRLRNTHGPSWLAQYSPKGETQ"
BASE COUNT         2145 a         3119 c         3420 g         1838 t
ORIGIN      
        1 ctgagtcgga ccgacagcta cccctactac ccctacccct gagagctaaa cccataagcg
       61 ccatgtggtt agcccaaaac gctaccccta cccctacccc tacccccgac agaatgacga
      121 agtacgaacc tggacagtgc cacactcacc acgttccggc ccgtgatacc ctagcggatc
      181 tccacaaccg cttctccgct ttacgagcct gcggatgcac cggagacccc gccgggttcg
      241 agccgcctcg ccgggatcct gcatgccgcg ctcatgcgga ctggcccgac gacgccgggt
      301 gtaagaattg cggcagctgc ggtgagattc cagccgggat cgcgggcaac ggcaccgcct
      361 acgtgcggga ctgcccaaag tgcgcggatg gggcagacaa ggcacgaacc ggccccacca
      421 cagaagagat cgccgccgtc aagccgtccc gtaggtgctc cacgggcgtc caccacgact
      481 ggaataccga ccgatacgga gccaactccc ccccacacga tccaagcaag cgggcaaatc
      541 cttgactcgg cacgccagac ttggcaagta ggccgtgagt caatcctcaa ccgcgcgggc
      601 cacgagatgt cattcgtcat aaccagcgac ttcgacttta cgggcgacaa cgtcatcgtc
      661 gcgtactcga caaacgagta cgtaatccag atcgttgacg aggccggcga cccgttcgat
      721 ttgacgccgt acgaagaggt acgtgccgag tttcgtaacc actcgaacga ggaccaactc
      781 cctggatcgc aggcgtccga tgttgtgtac gacgctgact acagcgcgac gaacatcacc
      841 ccagagacac atacaggatt cgtgtctccc aaaacctcgg gacagatccg agtcgttatc
      901 ggcgccgtcg agaccaaact ggagcaagac ggaagcgccc gcaaatccgg cttgtgggac
      961 gcggtggcct acaccactgc gaccggagca gacgagtcgt tccagaccaa ggtagcgaaa
     1021 ggcgcttgga gtacgcagtg gacctcgacg ctacagacgc cgtagaacat ggctaacatc
     1081 tccaccgttt caagcccgac cggactccga tcacgcggca agattacaac cgatccgccg
     1141 aacatcctcg tcatcttggc tgacgacgtt gggatagaca tgctggctca gtaccaagcc
     1201 tacatgatcg ccaacgccgg attcccgtcg ccagccgtca gctacgcaga cacgccgaca
     1261 cttcagtcaa tgaagacggc cggtgtgcga ttcatgaaca tgcgcacaca gccgaactgc
     1321 tcgcccactc gtgcgtgcat gctgagtgga cggtatgcgt tccgcacagg aatcagcgcg
     1381 gttgttcgtg aagcaaacat ggacctgacc gcgaacgcat gggagtttgg gatcaaccac
     1441 gcgacagacg agctattcat gccacgtctc acgattccaa agggataccg ctgcggcatt
     1501 ttcggcaagt ggcacctcgc cgctccggac ggcggcaccg ttccgaacgg agtaccgtgg
     1561 gagggtggaa ccccagcagg atacggaacc ggatggacac acccagacac catcagcagc
     1621 accgacggac agcatatcga ctacgggctc acgttctcga atcttgacat cgaaccattg
     1681 cccgccgaca gcgggtccac aaagcccgga tatcacaatt tctactggag atccaagacc
     1741 gaaggcaacg acgcaaccac aacgcaagtt gcggacgagt accacacggc atactcgcgg
     1801 caggaactcc aaagctggat ccaaggccaa accgagccgt ggctcgcgta ctgggcactc
     1861 tgcgcgatcc actcgccatt cggcacgttc cctgacaacg gaggaggcaa ctctggatgc
     1921 atgccacccg atgacgggac gacagcaact gactgggata cgtacaacga caccatcgga
     1981 acctatccgg tctacaactc gtgccgagct gccatcgaag cgatggatta ccagatcggc
     2041 ctactcaaga ctgcgcttgg agacgagtgg gaccgaacga tggtcctatt catcggagac
     2101 aacgggacgc aaaggaacgt tctcgtggcg atgcaggact gggagaccgc gaacgcgaac
     2161 gcgatcggcg aaccgtacgc gtcgttgctg tcagaggatg cattcaaagg caccgtgcat
     2221 gaggccggcc tgcgcgtgcc gatgatggtg accgggccgc ttgtcgcgag cccgaaccgc
     2281 gactatctcg gactggttga tgcggtcgat gtctatgcaa ccatcaggca tatcgccagg
     2341 gttccgtaca gctcgaccat ccaagacgat cgagtcatcg acggcatctc attccttcac
     2401 gctctgcaag acggaaccct tgatgtcgaa gggtcgcgtc aattctcgtt ctcggagaca
     2461 tacatcccgc tgggcgcggc aactggagca acagacacgg acgctgggca ccaacgcgac
     2521 cggagctaca ttctccacgt cgaaggcgac ggagtgtaca agctcaatcg agagttgtct
     2581 ggtacagtca acgtggacac gttcttccag ttgatggacg acgactacgt gcccgtagac
     2641 ccgtacatgc agaccccact ggcgacaggc gctggcgttc caaactacgt cgcttgcgcc
     2701 caagccatgt ccgcactctt cgcgaccgaa acggagtcga tcgtatagcc atggcgcgaa
     2761 ccatctcagc gacggcagta gcgaacggaa tcagttaccg tctcgtccga gacaccgaca
     2821 cggcaggggc aaaccaatac tggttcgagt cactgtctac cgacatggcg ctcgggcaga
     2881 agtggagtca actcaacacg tcgtcagtca gtaccgcagc gggcgcgaac gcgctcatcc
     2941 ccaccgggat tccaagcgcg gcactcgcgg cgattttcga gaaactggtg gccggccgat
     3001 gatctcgcca gaccagctcc tcgcactgcg ccgagccctc acaggggaac ccgtgccgcg
     3061 tccactccgg gcatccctac gaagatcgac cttctgcgcg tccgacggca ctatcaccga
     3121 tacggggcgc gccgtggccc gatctgaggc cgcacagcgg gttcaggcgt gctgcgtatg
     3181 caccgggccg gtcgagaccg cctacactat ccccgacggg tccgagatat gccccgggtg
     3241 cctgatcctg gtcgggaagt cgtgcaaggg gtctcggacc ggggccgatc ggtggactcc
     3301 gccgtggtgg aactcgattt tctagggtct tgacgcgcgg acgggatgcc gctatgggct
     3361 tcgggtgcag gaaaagccgc agaaaaagcg cggtcgggga cagccgacca agcgtacccc
     3421 cgagaatgcg aagctgattc tagacgcggt gcggctcggg ttgaccgaag atacggcgcg
     3481 acaactggtc ccgctttccg actccgcgtt gacttcgtgg aagtctgagg acccagattt
     3541 cttggcgtct ctaacgcgag cgcgagcaga cgggaagtat cgcaactcaa atcggctcga
     3601 tgctcggatc gaagcaggcg acacaaaggc cctaaccttt tggctcgaac gccgctgccc
     3661 ggagttccag caagagaagg aagagaagcc taagaccgtc gccgagcaag ctgccgatat
     3721 ggtcgcgatg taccacgcta tgaggacgct tgatggtgtc gatgactcag gctcctccta
     3781 agcgcgtcga tcagctccgg tatcatccgg tccaaagtcg cctgcggaag agcaacgcgc
     3841 gattcaaggt agtcgaagcc ggccgccgat caggcaagac cgaacacgcc aagcgcgacg
     3901 gcatccagcg cgcgatctgc gcgcacatgg atccgggcat cgacgactac cgcgttgcct
     3961 tctgcgcgcc gacgcgcgac caagccaagc agatttactg ggacgacctc aagcggatga
     4021 caccgcctga gttcgttgtc tcgatcagcg agaccgaact gtcgatccat ctcgtcaatg
     4081 gcgctatgat tgccgtggtt ggactcgata agccagcgcg catcgaaggc cgcccgtggg
     4141 acgatatcaa atgggatgag gtggccgact gcaagccggg tacgtgggac cggcacctgc
     4201 gacctgcgct tgatacggat ggccgaccgg gcagtgcatg gatctacggc gttccgcgcc
     4261 cgtcgtctga gttcaagcgt ctcgccgatc ttggcaagga cgagaacgag ccggattatg
     4321 agtacttcac ctggaagtca gcggacatac tcgcgcctaa agtgattgcg gctgcaaagc
     4381 ggacgatgga tccgctcatg ttcgcgcagg agtacgaggc ctccagagtc aatctccagg
     4441 gactcgcgta ttaccaattc aatcgcgagc gcaacttgcg cgaactgcaa ccagatacgc
     4501 gagcccctct catcttttgc ttcgacttca acgtgcagcc gggagtctcg gcggttgcac
     4561 aggagtttga ttcgcgcgaa cttggcaagc ctccggatgg gtgccagtgg gctagcgatt
     4621 cggtgacggg gtgggtgggc gaggcataca ttccgcgcca ctcgaacaca caccgcgtat
     4681 gtaacgcgct gatcgaatct tggggagatc acgagggact cgtgtacggg tatggggatg
     4741 cgactggtgg tggacgctcg accgtacagg gaaaggaaca gtggggatct gactgggcga
     4801 tcatcaacga cctgatgggt cgtcactttg gcgacaggtg gcgtgattac cacaggcgca
     4861 gcaacccgcg tgaacgcgct agagtgaatg ccgtcaatgc ccggatgtgt tctgctgata
     4921 acacggtggg cacagtgata gatccgacgc gcgccccacg gctgatcgaa gaccttgaac
     4981 taactatgat cctcgaaggg tcggctggcg aactggacaa gaagagcgat ccggccgcaa
     5041 cgcacctcac cgatggtgcc ggctactaca tcgagcagga attccctgcc gtttcgcgtg
     5101 acattgactc ggagcctttc aactgatggc aatcgcaagc gatacggtag acactccgag
     5161 cgcagtgtgg gaggagctat acaagcggtg ggagttgcct ctggcgctgc gcaaaggaac
     5221 cgacgggatg cgcgcaggcg gggtcaaatg gctcgatcgc gaggaggccg agacgcaagg
     5281 gcagtatgcg gcgagactca agcgcacgtt cttgcacccg gcattcgacg ataccgtcga
     5341 gcgtgtcgtg gcaaagccgt tctcgaagcc gatcacgttc cgtggggagc tgcccgagcg
     5401 gcttatgccg atgctcgaag acgtggacgg cgagggaaac aatctggacc agttcgcgtc
     5461 tgagttgatg ctctccgccg ttactacagg tatgtcgcac gtactggtgg acttcccgcg
     5521 agtagatagt cccgtatcgc tggcggaaga gcgggatatg cgacttcggc ctcggttcgt
     5581 acacgttccg gctgaacggc tgtttgcgtg gagttgggac cggatgccga acgggggcat
     5641 tcagctttcg tcgattcggt acaaggaaac gcgtgaggtc gaggttggcg cgtggggcag
     5701 cgagctgcgg aatctgatcc acgtttacag gttcaaccat gatggcggct cgacgtatca
     5761 ggcgtgggag cagcaatcgg acaggatcac ggaaggctcg tatgtcccgg tcgaggatcc
     5821 ggtgccgatc acgtttagcg gggtgcctct ggtcacttac tacacgggcc gtacgggtgc
     5881 gatggaggcc gcgccgccgc tacaagggat tgcggacctg aaccttgccc attaccaagt
     5941 cagtagtgag catcgaaacg tgctcaacgt ggtcggcgtg gcgatgttgt tccgcacggg
     6001 agtagacgga aaggccaagg acgccaagac agtcatatcc ccgcgcgcgg tctatacgag
     6061 ttcggtgccc gatgcgcgca tggagtgggt cgaacactca ggcgcggggg tcgaggcaat
     6121 tgagcggtac ttgaagcaaa ccgaagaccg catggaagtg ctcgggatgc agccgttcaa
     6181 tagcggaacg aggctgacgg caacccagcg cgtcatggac gaaagcaaga ctgagacaac
     6241 cgtacaaaca tgggttcgcg gactggaaga tgcgctgacc gaagcgttcc gcaaggccgg
     6301 tgagtggatc ggtgaggagg tctcggaaga cttcgcggtc caggtctaca acgactttag
     6361 cgttggtccg cgtgtcgagt cggacttgcg cgcgctggac tccgcccggg ctcgccggga
     6421 catcgacaag cgcaccttcc tggaagaact ccagcggcgc ggcacgctgg accaggacag
     6481 ggatctggac gagatcatcg aggccacgat ggatgagggg ccggatatgt ccgcgctcgc
     6541 gggggtggtg tcggcgctgg gcgcggacga aactgaggaa gccgcgtgaa tcggcacgat
     6601 ggtagggtga tggtgtgatt cgccaagccc tctatgtggt ggcaccctgg atgctcccgc
     6661 tagtcgcgct ggcgatcatg gttgtcatgc ttgcgatggt gggcggtgcg tagtggcgtc
     6721 ctaccgcccc gagatattca tccacatccc gaagacgggc ggcacaagca tttgcaacag
     6781 cttgcgggtg gaggggaagc acacgccgtt gagccatcga cccgagggtg ggttccggtt
     6841 cacgttcatc cgcaacccgt gggaccacgc gcgttcgtgg tggcaccact gccagtacgg
     6901 gaagcggacc agcttcgagg gctgggcgct tgatgggtgc ccgggtacgt ggaccgtcgg
     6961 ggacacgggt gtccacatgc cgcgtcaagg cgactggttc gagcggctgg gcgatccgcc
     7021 gatggacttc attggtcggt tcgagtcgct ggatgccgac ttcgggcgat tgtgcgtact
     7081 gattggggtc gaagggatca cgctatcgca cgacaacgat cggggtggaa gcaagcggcc
     7141 tcactaccgc gaggtgtgga cgccggccat gcggaacgca acgccgtggc tggacgagtt
     7201 cgcggggagg tatgggtatg agttctagct acgacaagat agaggccgtt gaaatcgctg
     7261 tgcgccgcgc tttggtttcg gttagatcgc atgctgacac cgtccaagtg tttgcaacat
     7321 gggtggacga ggaaggcgct acgcattgct actctcgcgg cgaggggaac tggcatgcgc
     7381 gcatgaacca agctcgaacg tgggtgcgcg aagaggatgc tgctgggatt catgagcgga
     7441 tccagggtga tgactgaccc ccgcctcctc gaccccgcca agaccgcgaa cgagatcctc
     7501 caggatcgcg cgatccggca tgccatctac atcgagcggc tcaaggtcgg cgaggtccgc
     7561 tccacactcg cggaattgaa cgcgctctat gatgagctgg tagcgcgcac gttcaaggtg
     7621 ctggatcgta tcgcaacgcg cggcgcgaaa gtacgagagt tcaagtcgat ccgcaacctg
     7681 acacgcgacc tcaatcggat tgcggtcgat cagtacgact cggtagcgcg cgagctgatg
     7741 ggccgcatgg aagcgcttgc ggctgcggag tctcgctggc agctcgcggc gatcacggag
     7801 agcgctacgg ttccgctcac gctcaaggcg ccgtcgcgtg cgatcctgcg ggccatcgtc
     7861 cacaatgagc cgttcggaga tccgacgcgt gcagtggtgc ggacgttgcg cggatggttc
     7921 gaggccgagg gcgagatgtc ggcatttcgg atccagtccg cgctagacgt tgggttgcgc
     7981 cagggcgaca cggtggcgcg gattacgcgg cgcgtgcggg atacgttcgg cgtgagcggt
     8041 cgcaacgcgg agacgtgggt gcggaccggg atcaataaca ccgtgacgct ggcgcgggag
     8101 cggacgtacg aagagaacgc ggaagtagtg aagggcgttc agttactcgc gacgctggac
     8161 agtcggacga cggtgatttg catgggcgag gatggcaacg tgtacccgat cagcgagggt
     8221 ccgcgtccgc cgttgcatcc gcgttgcagg acaaccacgc tgcccgtgtt gaagtcgtgg
     8281 aaggaactcg gcattccgct caaggagttg cccgagggta cgcgcgctag catgaatggg
     8341 caggtcccgg agtcggtgac ctacggcgag tggctcaaga cgcagagccg cgagttccag
     8401 gacgatgttc tcgggccgaa gcgcgcggag ctgttccgaa ccggcgcggt cccgattgac
     8461 cgcttcaccg atgagcggct ccggccgttg acgttgagcc agatcgtaga gcgcgagaaa
     8521 ctttctccgc tgacggccgg cgcgttcaat cccaaagcac ccgagacaat cggccagtac
     8581 cgagcgccga atggagtgtg ggcaccggag cggcagaagc ttcatgacga gattgtgcgc
     8641 gagcatcttg cgggcttgcc tagccagaag aatccgacct tccacatgat gggcggaggc
     8701 cccgcttcgg gtaagtccac cgtcatcaag acaggcgatg tcaacctacc gcctggtaac
     8761 gtgctcatcg actcagacgc gatcaaggcg aagctgccgg agtaccgcgc gatgcaggcc
     8821 gcgaaggctc cgggggccgc ggcgttcgcg cacgaggagt ctagctacct gtcgaagcgc
     8881 atcctcgcgg aggcggggga tcggcgaatc aatgcggtgc tcgatggtac gggcaacgga
     8941 agcacagccg ggatcttcaa gaaggccgga ggcatgcgcg agcggggcta tcgtgtcgtg
     9001 gcggattacg ttaccgtcga caccgagacc gcgctcgctc ggaacctcgc acgcgccgcg
     9061 aagacggggc gcatggtgcc ggccagctac gtctctggtg tacacgaaac gatctcgcgt
     9121 atcctgccgg atgcgatcga gggcggggcc ttcgacgact tccggttgtg ggatacaaac
     9181 gcaagcggtc cgcgactcgt agcaaacttg gaagggggcc gattgaagat cttcgacgaa
     9241 cggctatggc tcaggttcct acagaaagca ggggggtagc atgctgaccg cggaagacat
     9301 ggcgcgcatc gtgaaggaag aaacgctcgg gatcgacgcg cccacgaacg agacgcagga
     9361 ggctgcggcg ttccgcgtga agacggccgc gagtgttgcc gaggttcact cgctcgggta
     9421 cgaggtggag atcccgcaag agtggcccga catcagcgac ttccctacga aagagccccg
     9481 cacgggcggg gcctgactcg gagcgtgcgg gcggtgggct agaggccggc cggcagactc
     9541 tccctcatcc cacaccacga tccgccggac cagcggaaac ccgcggcctt gagggcgtcc
     9601 agggtcgcgc gtgcgggctt ctcctcgaag gtgacccgaa cccagtcgcc tccctcgatc
     9661 agcgtcccgc cggcctccga ggcggcctct gcgcgacgct ggcgatttgc cacgtcgacc
     9721 gcgcgggact tgaggcgccg gatgttcgcg cccaggttgg agagttggta gctctcgaac
     9781 gggacccatg ccttgctgta tggccacatc gcgcgggcct cttcgaggcg ctggcgcttc
     9841 tctgcgtcga agccttccgc ctcgcagaag gcgcctaggt cgcgcttgca cttccgatag
     9901 gaagcgttga ggcgcttcat gttgtcgcgc ttggcttcga gttcggcgac acgttcggcc
     9961 agcgcatcga gcgcgtccgg gtcgtccgag aagatggagc cttcgagttg cgcggacagc
    10021 ccgccagcct tcgagcggtg gtggtctgcc atgctggcgt gctcgacgcc ggccgacatg
    10081 ccggagcgga tgcgttccac gtcgcgccgt gcgcgcttct cgctgtggtg gccgacgagg
    10141 atcggctggc cgagcgggat tgagttggcg agcttgcgcg acgcggcgaa ggcggcgtcg
    10201 gacttccggt cgcgtgtcgt ggcccagtcc tcgcgcttct ctagcttgcg ttcgaggcgt
    10261 tctctgcggg tcattgagtc tctcctttgg ggctgtactg agcgagccag ctcgggccgt
    10321 gtgtgttgcg aagccgctgg aggattccgg gctctgcgga gcgcatccat gcggagcgga
    10381 ggcgggcttt ccattccgcg ccgttggctt ccgcgtaggc gcgcacggtc tcggcctctt
    10441 cgtcggtcag ttcggggaag gtctgtttct tgggcattgg gctgctcctc tagttcagtg
    10501 tctcggcgac gaacgcggcc tc
//
LOCUS       DASRZF010000305         2509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3327743, whole genome shotgun sequence.
ACCESSION   DASRZF010000305 DASRZF010000000
VERSION     DASRZF010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3327743"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VFG22_13280"
     CDS             complement(<1..528)
                     /locus_tag="VFG22_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6417262.1"
                     /translation="MSKAEAAVEERPSNVKMETRNVNVFYGTNQAIRDVDLDIADRSV
                     TALIGPSGCGKSTYLRALNRMNDTIDICRVEGSILLDGEDIYAKGVDAVEVRRRVGMV
                     FQKSNPFPKSIFENVAFGLRIGGEKDKDLIAERVEESLKGAALWTEVKDRLQSSALGL
                     SGGQQQRLCIARCLAV"
     gene            complement(525..1424)
                     /gene="pstA"
                     /locus_tag="VFG22_13285"
     CDS             complement(525..1424)
                     /gene="pstA"
                     /locus_tag="VFG22_13285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET6417263.1"
                     /translation="MRHTKKDWLLAGLTGLSLAIILLLLAVLLGQIIWHGAPVITVEF
                     LTESPGADMVSGGIWPAIYGTVFMTLLMTLAGVPVGIATAIFLSEYASPRSRLAKLVR
                     IAVNNLAGVPSIVFGLFGLGFFIIFVGGSMDSLLYDDPTPVWGKPSILWASLTLAVLT
                     LPVVIVTTEEALRTVPSEIRDASLALGATRFQTVYKIVLPNAKGGILTGVILAVSRGA
                     GEVAPIIFCGAANFLPYLPTDLRDMFMHLGYHVYALATQSPNVDAAQPTLFGTVLVLL
                     VLTFSLNLLAVILRARSRAALRP"
     gene            complement(1421..>2509)
                     /gene="pstC"
                     /locus_tag="VFG22_13290"
     CDS             complement(1421..>2509)
                     /gene="pstC"
                     /locus_tag="VFG22_13290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET6417264.1"
                     /translation="RGWLLPASKGSPSGRRVTDQLLDAADGGAASKKRQRVALGKRPA
                     KSKLVSSFRTPLWHRVVERMIAVLALGAIAAIALIFIFIAREAAPMFWETEFAQEVEL
                     RDLFFAKEWKGYEEPVFVWQPVGEPAKFNVVPLFVGSLKVTLLAMLMSIPLGIGCAMF
                     IALYAPPMLREVLKPTIELLAGIPSVVLGFFALMVLASWAQATFGFHFRLNAVVAAIG
                     LALTIVPVIFTISEDAIQSVPKTLTEAALALGARKYQVALRVVLPAALPGIAAGVVLG
                     FGRAIGETMIVLMASGNAAVMELFDFSTSVRTVTATIAAELGEVAQGDPHWRVLFLLG
                     VMLFAVTFVLNVLGDLFIQRLQKKLTAS"
BASE COUNT          531 a          760 c          770 g          448 t
ORIGIN      
        1 aacggcgagg caccgcgcga tacatagccg ctgctgttgc ccccccgaca gcccgagcgc
       61 tgagctctgg agccggtcct tcacctcggt ccacagagcg gcacctttga gggactcctc
      121 caccctttcg gcgatcaagt ccttgtcctt ctctccgccg attcgaagtc cgaacgcaac
      181 gttctcgaag atcgacttcg ggaaggggtt cgatttctgg aagaccatgc cgactctgcg
      241 gcgcacctcg acggcatcga caccctttgc gtagatgtcc tcgccgtcca aaagaatgct
      301 cccttcgacg cggcagatgt cgattgtgtc gttcatccga ttcagtgctc ggagatacgt
      361 gctcttgccg cagcccgagg gtccaatcag cgcggtcacg ctccgatcgg cgatgtcgag
      421 gtcgacgtcc cggatggcct gattggtccc gtagaagacg ttgacgtttc gcgtctccat
      481 cttgacgttc gagggacgtt cctcaacggc tgcttcggct ttgctcatgg tcggagtgct
      541 gctcgcgaac gggcgcgaag gatcacagcc aagagattga gcgagaacgt gaggacgagc
      601 aagacgagga cggtaccaaa gagggtgggt tgagccgcgt cgacgttcgg ggactgggtg
      661 gccagcgcgt acacgtgata accgaggtgc atgaacatgt cgcgaaggtc ggtcggcaaa
      721 tagggcagga agttggcggc accgcagaag atgattggcg caacttcgcc agccccacgg
      781 ctcaccgcca gaatcacccc ggtcaggatg cctcccttgg cgttggggag aacgattttg
      841 tagacggttt ggaatcgcgt tgcccccaga gcgagagagg catcccttat ctcgctcggg
      901 acagtcctga gggcttcctc ggtggtgacg atcaccaccg gcagggtgag caccgcgagc
      961 gtgagcgagg cccagagaat cgagggcttt ccccagacgg gcgtcgggtc gtcgtagagc
     1021 agggaatcca tgctgccacc cacgaaaatg atgaagaaac cgagtccgaa gaggccaaag
     1081 acgatcgacg gaacccctgc caggttgttg accgcgatgc gtacgagctt cgccaggcgt
     1141 gaacggggcg aagcgtactc actcaagaag atcgccgtgg cgatgccgac cggtactccc
     1201 gccagcgtca tgagcaaggt catgaacacg gtgccataga tcgccggcca gattccgccg
     1261 ctgaccatgt cggctcccgg gctctcggtg agaaactcca ccgtgatcac cggagcaccg
     1321 tgccagatga tctggccgag aagcacggca agaagcaaga gaatgatcgc gagggacagc
     1381 cctgtcagcc ccgcgagcag ccagtccttc ttcgtgtgtc tcatgaagcg gtcaacttct
     1441 tctggagccg ctgaatgaac aagtctccga gaacgttgag cacgaacgtc accgcgaaaa
     1501 gcattacacc gagcaggaag agcacgcgcc aatgcgggtc tccttgtgcg acctctccta
     1561 gctcggcggc gatcgttgcg gtgacggttc gcacactcgt gctaaagtcg aacagctcca
     1621 tgacggcggc attgcccgag gccatcagga cgatcatcgt ctcgccgatt gcacgtccga
     1681 agcccaggac cacgcctgcc gcaattccgg gcaaagcagc cggtagaacc actcgcagag
     1741 cgacctggta tttgcgcgcg ccgagggcca aagcggcctc ggtcagcgtc tttggcacgc
     1801 tctgaatcgc atcttcggag atcgtgaaaa tcaccgggac gatggtgaga gccaagccga
     1861 tcgctgcaac gaccgcattc agtcgaaaat ggaatccgaa agtcgcctgc gcccaggatg
     1921 ccaggaccat cagcgcaaag aagccgagca ccaccgaggg tatgccggcc aggagctcga
     1981 tcgtgggctt tagcacttcg cgaagcatcg gcggcgcata gagggcgatg aacatggcgc
     2041 agcctatgcc aagtggaatg ctcatcagca tcgccagcaa ggtcaccttg agtgagccga
     2101 cgaagagcgg aaccacgttg aacttcgccg gttcgccgac tggctgccaa acgaagaccg
     2161 gttcttcgta gcctttccat tcctttgcga agaagagatc tcggagctcg acctcctgcg
     2221 cgaattcggt ttcccagaac atcggcgccg cttcgcgcgc gatgaagatg aagatgaggg
     2281 cgatcgcggc gatcgcaccg agcgcaagca cggcgatcat ccgttcgacc actcgatgcc
     2341 acagtggcgt acggaacgag ctcacgagct tggatttcgc cggtctcttg ccgagcgcga
     2401 ctcgctgccg cttcttcgag gcggcgccgc catcagcagc gtccagcaat tgatcggtca
     2461 ctcggcggcc gctggggctt cctttggaag cgggtagtag ccaacccct
//
LOCUS       DASRZF010000306        19318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3329249, whole genome shotgun sequence.
ACCESSION   DASRZF010000306 DASRZF010000000
VERSION     DASRZF010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 19318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19318
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3329249"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..587)
                     /locus_tag="VFG22_13295"
     CDS             complement(60..587)
                     /locus_tag="VFG22_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417265.1"
                     /translation="MTSRLGLVICLCASLGCSDGGSAPSSGPIVDNTQWVPSSEGEAI
                     FGPQPEGSGCEPTPIDCEDYYPWPPGECVEFQANASCIAAYIPECFPPSYTVLAIYTR
                     MPDDGISLCDWLTLEQPSLQAIRAGDVIEVRAYHNELTAPVPGEARMSFAVGDQLAFD
                     ETILIPNASEFLTGT"
     gene            complement(624..1151)
                     /locus_tag="VFG22_13300"
     CDS             complement(624..1151)
                     /locus_tag="VFG22_13300"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HET6417266.1"
                     /translation="MARYDAYNSECLLFSFKDGLLARLAHDLKLQVERFSIEVDDTTH
                     QIKAAFDPSSIAVVCAMADGRDDPSTLSKGDKKKIYDNVTKDVLKTRKHPEIRFDSTN
                     VVQRGEGFAVEGTLQIQGKSRTVQTSIRPDGDRWVTELTVNQPDFGIKPFTAVLGALK
                     VKPEVIVRLSVPRQT"
     gene            complement(1211..3136)
                     /locus_tag="VFG22_13305"
     CDS             complement(1211..3136)
                     /locus_tag="VFG22_13305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417267.1"
                     /translation="MKPVQYAFAAFAVLSVAACGSDTSSDSAQKCDADSTFAQIQQQI
                     FDGRGCTASACHGEAANAGLDLRPESAYANLINVEATSGDYMRVFPGEQDLSALYQKV
                     AAKTEGFELSALPNPISGGAMPTGNGALSEDDLALLRAWIRGGAPEAGIVEGSQQYAS
                     CQLAGDLAPNKIQPLPAPATDEGVQFYSGGWAVPAEGEGEVCFITYYDYSDQIPAEFT
                     VPCGEAQGGPEQDCFVYDEVLLAQDPQSHHSIIEFYVPPRVCVGGDNDGDGCDQDESV
                     CGDGAVCVLNPDHLDPTNAVWKNWQCLGGDLAGTSCTPGSDECGPRGQCATEPLTTIA
                     CINYRNAPQELGTIAGFFGRTTVRQNLATAQESSFREAFPPNVFSMVPVKGFVVWDSH
                     AFNLTTADTTVEQWINLTFAPSGERLYPRTQIFDADDIFGMGRIEAYSSGEACASFEI
                     PQYGRVMTLSTHTHRFGKDFRVWYPPNEVCDASSNPTAPNEDPCTRPDREADYVSFDY
                     ADPLYQRFSEDDILRFDSPNKEDRTFVYCSVWDNGESNPSEVRRESMKPDAETCEFVD
                     QFAPLANQAGLGLFACGCVLEDRSCFGGPNEGMACNGEDSVCGEGGICDACPLGGGVT
                     TEEEMFILLGSYYVETP"
     gene            complement(3263..4981)
                     /locus_tag="VFG22_13310"
     CDS             complement(3263..4981)
                     /locus_tag="VFG22_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417268.1"
                     /translation="MFLTACGGTDSDGGSTPKDCNYASTYDAIQATVFEAKGCTASAC
                     HGDQMQGGLDLRADASFDSLVRQPSTIEPSIERVYPGDQDLSLLYSKLAAGTEGTDLG
                     SLGQPMPSGGEPLSIDQLEAMRLWIRAGAPSDTIVKQTLELLGCDGTFDPQPNKIAPL
                     PPPAANEGVQFYAGGWDLDAEAEDEVCFASYYDFTEQVPPEFQVDCEQFGAGRKCFAF
                     RRNELAQDGQSHHSIINVYTPESDPNGGDWGPWACLGGEKAGQSCTPGATDECGPRSQ
                     CTTPAITRVACVGYPYAPVDFGLGGGVGGQTATQVQLSGAQESTQIDLPPAGVYSVLP
                     LRGFVSWNSHGFNLTKYATSIEQWVNLTFAPEEERVWLRRQIFEADNIFAMTEVEPFT
                     KREVCMTWTLPQYSQLLSLSSHMHMRGELFRIWLPPNTPCQGTNGCVPPSSAPDYKSQ
                     LYDDPLYTYYEPPNDYSSASVEDRTLKACAVYDNGADNPLEVKRESTKPNTPACDFFL
                     ASCGCAAADRVCLGGENEGTRCNGDDSVCGEGGLCDACPLLGGVTTDDEMFIPLGSYF
                     VKPPSP"
     gene            5145..5591
                     /locus_tag="VFG22_13315"
     CDS             5145..5591
                     /locus_tag="VFG22_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABA4-like family protein"
                     /protein_id="HET6417269.1"
                     /translation="MTLEMLLQAANYAVIPFWLLLILAPRWSWTERLVHSPIVLLLLT
                     PIYAYMLFGYGPAPEGMSFSSLYGIMVGFSAPHVAVAGWIHYLIFDLFVGAWEARDAR
                     RRGVPHLLLVPCLLATLLIGPVGLLLYVLVRFFSKRLLEFDEAGVQ"
     gene            complement(6012..6779)
                     /locus_tag="VFG22_13320"
     CDS             complement(6012..6779)
                     /locus_tag="VFG22_13320"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HET6417270.1"
                     /translation="MQLRVLGCHGGETPKHRTSSFLIGDTLAIDAGAITSGLSLDEQA
                     RIDSVLVSHPHMDHIRDLATLADNRCQQGGSTLDIVGVPATIEALRIHFFNDLIWPDF
                     TKIDAKDGPTVRFVEVHPNRVAEVNGYEVTPVMVNHTVDTSAFIIRQNASSIVYGGDT
                     GPTDELWTRINALDDLQALMMEVSFPNDESELAFQSRHLTPDTLSTELSKLELSEELP
                     ILLYHIKPTFEAKVLRELAGVRGRNLQILQLEDEFLL"
     gene            complement(6779..7405)
                     /locus_tag="VFG22_13325"
     CDS             complement(6779..7405)
                     /locus_tag="VFG22_13325"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET6417271.1"
                     /translation="MTDQSLADKFGVRLSRGATVFRQGDPGGSMYVIRAGKVRVLKDT
                     QGRQRVVTTLGPGDFFGEIAVVTGRPRTATIEVLEDVELLRVPADKLQEMVSGTGEVA
                     IRLIRHLAERLENANRFIDLLLEDDVTARVILELQETLQNAEGAAAPDVTDKDIALKL
                     GIDKNDVRSALRRLTRVGILEVSSGFVLIKDTVRLGEFLEFIRSSGES"
     gene            7483..8943
                     /gene="cls"
                     /locus_tag="VFG22_13330"
     CDS             7483..8943
                     /gene="cls"
                     /locus_tag="VFG22_13330"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HET6417272.1"
                     /translation="MSFDWRQVQDIAPELSVILDLVIAAGASSHAILHKTDTRSATAW
                     VGLIWLLPFVGAMLYLFLGINRIQRRAKELKSGVVEYSVHLDQAPRSPADLDRELGSE
                     RARLVELARVVGQVTNRPLLVGNQLDRLVDGDEGYPAMLEAIADARTSISLMSYIFEA
                     DSVGGEFIDALADASARGVEVRVLIDDVGTRYGRPRIHRVLAKRRVPVAKFLPAFLPR
                     SMTHFNLRNHCKILVVDGRVGFTGGMNIRRHSMIRADPKDPTKDLHFRVQGPVVAQLQ
                     GAFARDWAFTTGEHLAGKRWFPKLAPIGETLARGITDGPDQDIEKLQWVFLAAVGAAR
                     RSIRIMTPYFLPDTVLAKALHLAAKRGVEVDIIVPEVGNLPVVRWAMWGNYRQILEEG
                     MRIWLSPPPFDHSKMFLVDGYWSSIGSANWDPRSLRLNFEFNLECYDEDFGASLEEHF
                     RDRLRTVRLLTLEDLDARSTFKKLRDGTARLMTPYL"
     gene            complement(8934..9605)
                     /locus_tag="VFG22_13335"
     CDS             complement(8934..9605)
                     /locus_tag="VFG22_13335"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HET6417273.1"
                     /translation="MALAQLAVFVRPPMEGIAKTRLAGLLGAHGAAALYAAFVDDTLR
                     LCERVQAAGRVDIELWSTGPHDDTVSSWASRLGTTVRFQPQGNLGVKLAAAFEEGLQR
                     YERIVIIGSDLPTLPIELIASAFEALENAPLVLGPANDGGYYALGATRSIRPSFEGVR
                     WSTVHALRDTAEANASHRIARIAPWYDVDEPRDLSLLRAHLSVDESAAPATARCLAEL
                     ARAQR"
     gene            complement(9596..10282)
                     /locus_tag="VFG22_13340"
     CDS             complement(9596..10282)
                     /locus_tag="VFG22_13340"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET6417274.1"
                     /translation="MARVDVIIPALDEEQGIALVLRAMPKSLVRRIVVADNGSRDRTA
                     AVAREHGAEVVYEPERGYGAACLRAMSHLAADPPEIIVFLDGDYSDHPEELPSLVQPI
                     LRGEAGIVIGSRAIGRRARGALSPQQQVGNAIACGALRALYGVEYTDLGPFRAIRWDV
                     LRSLRMQDRNYGWTVEMQIKAAKLGVSYREVPVSYRSRIGVSKVSGTIRGSVGAGTKI
                     LWLLGRYAWR"
     gene            complement(10292..10912)
                     /gene="rpsD"
                     /locus_tag="VFG22_13345"
     CDS             complement(10292..10912)
                     /gene="rpsD"
                     /locus_tag="VFG22_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HET6417275.1"
                     /translation="MSRYTGPRVKRMRALGIDLPGLSRKSMWDRPYPPGVHGPKSARR
                     RKMSDFKKQLLEKQKLRFNYGLNEGQFRRLYREAVHSKDPSGDKLLELLERRLDNFVF
                     RAGFAPTIPAARQLVCHGHFLVNGKRLDIPSYRVREGDIVTLRERSANLTAVDSSLED
                     VRLARPEWIEFDDGKKAATVKGLPKVESVPFAVEIDLVIEYYSKSL"
     gene            11073..12479
                     /gene="rimO"
                     /locus_tag="VFG22_13350"
     CDS             11073..12479
                     /gene="rimO"
                     /locus_tag="VFG22_13350"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HET6417276.1"
                     /translation="MAGPRTIHFVSLGCAKNRVDTEVMLGVSDLTGYQIVEDPAAATV
                     IVINTCGFIGPAKEESVETILEMSAHKGSGTCEKLVVAGCLSQRYPEELASELPEVDH
                     FLGSSDMLKLKKVLGGTAERMLVGNPAQYTMRAEDPRVLSGQTHRAYVKIAEGCNREC
                     SFCAIPSFRGKQRSRPIADIVREAEQLAAAGVVELNLISQDTIAYGRDLPQRETLADL
                     LTALGDVNGVHWVRAHYLYPEKLTDSLVEVFAQHEKVLPYIDMPLQHASDKMLRVMRR
                     GHGGERMYKVVERLRRAIPDLVFRTTFIVGHPGETDEDFAELRSFVEWAEFDHVGVFL
                     YSPEEGTPSGEMNDAVELSIGEARREELMGVQRPISRDRLELRLGTELEVLVEGVSDE
                     SDFLLQGRWWGQAPEVDGSVYLANGTAEAGEFRRVLVTDAADYDLMADFVDASGARDA
                     PPDAKDPRPAKVKLRTLT"
     gene            12476..13204
                     /locus_tag="VFG22_13355"
     CDS             12476..13204
                     /locus_tag="VFG22_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417277.1"
                     /translation="MRWTGLALVLVSLVAAGGCGVAVPSMSGASTVPKNRTDFGLGGA
                     ARVPLGALKDPAVLDPGESQYRYATDAGGVVPMAYGRYGIARNWDLGLMVSGATVRLD
                     ARYERVLREGTTRSALVVGMAPYGGWIPDRDQSGSGGRVGFEVPFVYGVEIGGVYELW
                     LGARGSGEYVFGDFQVQGSEQRASGAGLRIGPVVGMALGVRRVHALIELTAAYEYWFI
                     DHAGASLDRGGFVLIPGFGIRLRL"
     gene            complement(13209..13766)
                     /locus_tag="VFG22_13360"
     CDS             complement(13209..13766)
                     /locus_tag="VFG22_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417278.1"
                     /translation="MAAEEIECHVCGAGNPKGSERCQSCGARLSDVSHELSEEELYAR
                     RHQPDAFELRWVFISFGVFLSTAALALALLPLVISAYDPQGFPGIVITILIWFVGAAA
                     INYVSPGKNFLEPPVGGLLAAIPTMIFLSSIADVYQLSAGAYLLGILMATMMALMGAF
                     VGNLLRGDHAKPAPKKQRSNRPKAA"
     gene            13934..15805
                     /gene="aspS"
                     /locus_tag="VFG22_13365"
     CDS             13934..15805
                     /gene="aspS"
                     /locus_tag="VFG22_13365"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HET6417279.1"
                     /translation="MARFIDELKRTHSCGELREEHIDREIVLFGWVQNRRDHGGCIFI
                     DLRDRDGLTQVVFDPETTDKIAFETANKARSEWVLGIRGKVRDRGTKDDGSSLHNPRL
                     ETGAIEVIALEATIFNKAETPPFEIEDKITTGEDKRLEYRYLDLRRPVLQKNLIMRSK
                     LNHETRNYFNESGFLEIETPFMVRYTPGGARNFLVPSRLYGGHFYALAESPQLYKQLL
                     MCAGYDKYFQIVRCFRDEDLRIDRQPEFTQVDVEMSFINQDDLFKVLEGLVFRLWKEV
                     LGIDLLERYPSGEFPRLPFAESMDRFGNDKPDMRFGMEHIDLTDLTIEHGGGGIGMLE
                     PIAKKFSEGAYRRDLPMEIVKAMRVPAGHGFSRKDLDALEKYVGQMGAKGLARAKLDA
                     EGNWLQSPLAKMVTPEFRKAVNEKVGAEDGDLIFFQFGPTQKVHTVMANLRLHLGRKL
                     GLIPETGSSGDWNFLWVVDPPLFERNEEKKRWEAAHHVFTRPHDECIDLLGTDPGNVL
                     CYRYDLVLNGFEIAGGSIRLHDPEVQKKVFEVIGLSDEEAREKFGFLLDALTYGAPPH
                     GGIAFGMDRVAMLAAETESIRDVIAFPKTQRANDLMTSAPSRVDPGQLLELKIKTLE"
     gene            15912..16238
                     /locus_tag="VFG22_13370"
     CDS             15912..16238
                     /locus_tag="VFG22_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417280.1"
                     /translation="MKALLNFALVALCVLGMTFPEVGHADEPNDIKNCYSVHGEARYG
                     PLAYKHVVIVTNRCDLSLACEVWTDVDPTPKQSLTVEPKGTGEVIVRTSSPARAFKAF
                     GECKKQ"
     gene            complement(16245..17336)
                     /gene="dapE"
                     /locus_tag="VFG22_13375"
     CDS             complement(16245..17336)
                     /gene="dapE"
                     /locus_tag="VFG22_13375"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HET6417281.1"
                     /translation="MTEIAHALRDTLLALTRIPSPIGEEGQLCDYVEHRLKGSLGAGA
                     ITRFHDSLVVHAAKRENAARIALVGHLDTVRTQHDGPPRVEGNLLYGAGAADMKSGLA
                     IMIELVERLDLRALPCDLTLIFYEREEGPFEENRLGPILDNYPKLHELDLAICLEPSD
                     NQMQLGAMGSIHATLTFQGRTAHSARPWQGDNALYKAARFLAELEKRAPHDVDLDGYL
                     FREVMTPTVLQGGGPRNVVPGEAQLNVNYRFAPGRTPEQAFAELRDLAGNQCKVEATD
                     LSPAGHPHANDRYIQYLMSCGVNGVRSKQAWTDVARFDAIGVAGVNLGPGTAAQAHQP
                     NEYTELDKLVEGYAIFERFFKNAENNPLY"
     gene            complement(17371..17961)
                     /locus_tag="VFG22_13380"
     CDS             complement(17371..17961)
                     /locus_tag="VFG22_13380"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HET6417282.1"
                     /translation="MSIDAYVLLAIFVGGKSSRMGTPKGLLELPGASEPIIDALVRSG
                     RQAGFEPVLVGDAMPYAQLAAGVPRIDDDPLGAGPLAGLQAAVRHASQSGFSRLIVVA
                     CDMPYVTSRTLAYIAAHPSPAPVLAARRGHHSPWEPMLARYDTARLAPVLDEAIAGGL
                     RSFQQLFALLEVEALPLTSGITRALEDWDTPEDLPR"
     gene            complement(17962..18384)
                     /locus_tag="VFG22_13385"
     CDS             complement(17962..18384)
                     /locus_tag="VFG22_13385"
                     /inference="COORDINATES: protein motif:HMM:NF025384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4234 domain-containing protein"
                     /protein_id="HET6417283.1"
                     /translation="MTAPIIERREPALVLVLSIITFGIYLPFWYHGIYEDLEALDGRT
                     PTGHGFWLDFFFVILTLSIYGIWVDYRISQQIAEFEERNGLGPVQDTTIIAVILDVAA
                     YFTAFWTNFVTSAIQQDQINRIVEKMPAVVTNELGAPV"
     gene            18438..>19318
                     /locus_tag="VFG22_13390"
     CDS             18438..>19318
                     /locus_tag="VFG22_13390"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET6417284.1"
                     /translation="MDEGATQAATIAVIGDVHLFWDDEDVAFFNGAGYDLVLFVGDLA
                     GYTQVRGRRVARSLRKLRVPAMCIPGNHDGLHAFQLGAEISPRARPLRNAFCRGQAAR
                     CRNLDRALGEVELLAYSRRRLAPAGVPLNLVVGRPHSIGGRRLACMRYLAEEYGIDSM
                     DASIRRLQSLVDSCDEAPILFLAHNGPSGLGDRSDSIWGCDFRKKEEDWGDRDLEEAV
                     RYARGKGRKVLATVAGHMHRRTKSGKRRPAQVEKDSVLYVNAAEVPRHRNENGKKTRH
                     HVRLRVSAERAVAEDLW"
BASE COUNT         3660 a         5807 c         6168 g         3683 t
ORIGIN      
        1 ccgtggttgt cgacgtgcca gagcaggcgc gtcccggcgg ggtagtcttt gtcggcggtt
       61 caagtcccgg tgaggaactc cgaagcgttg ggaatcagga tcgtctcgtc gaacgcgagc
      121 tgatccccga ctgcgaagct cattcttgcc tcgccgggaa ccggtgcggt cagctcgttg
      181 tgataggcgc gcacctcgat cacgtcacct gcacggatcg cttgcagcga aggctgctcg
      241 agcgtgagcc aatcgcagag tgagatcccg tcgtctggca tccgcgtgta gatcgccagg
      301 acggtgtacg agggtgggaa gcactccgga atgtaagccg cgatgcagct cgcattcgct
      361 tggaactcta cgcattcccc ggggggccac gggtagtaat cttcacaatc gatcggagtt
      421 ggttcgcaac cgctgccctc gggctgaggg ccgaatatcg cctcaccttc gctgctcggc
      481 acccactgcg tgttgtcgac gatcggaccg gagctcggcg ctgagccacc atcgctgcac
      541 cccaacgagg cgcacaggca gatcacgagg ccgagtctgc tagtcatgac gcctccgaag
      601 atactcgcct gcgccggggc cgactaggtc tgacgcggaa cgctgagtcg cacgatcacc
      661 tcgggtttga ccttcagcgc gcctagaacc gccgtgaacg gcttgatccc gaagtccggc
      721 tgattgaccg tgagctctgt cacccagcga tcgccatcgg gacgaatgct cgtttggacg
      781 gtgcgtgatt tgccctggat ttgcagcgtg ccctcgaccg caaacccttc cccgcgttgg
      841 acgacattcg tcgagtcgaa tcgaatctcc ggatgctttc gcgtcttgag aacgtccttg
      901 gtcacgttgt cgtaaatctt tttcttgtcg cccttgctga gggtcgaggg atcgtctcgc
      961 ccatccgcca ttgcgcagac gacggcgatg ctgctcggat cgaaggcggc tttgatttga
     1021 tgggtcgtgt cgtcgacctc gatcgaaaac cgctcgacct gcaacttcaa gtcgtgcgcg
     1081 agccgggcca gcagaccgtc cttgaacgag aagagaagac attcggaatt gtaggcgtcg
     1141 tagcgagcca tcgtcggcga tgtagctcac cgccgaacaa tgggccagtt cgcttacagt
     1201 actattacag tcacggcgtt tcgacatagt acgagccgag aaggatgaac atctcttctt
     1261 cggtggtcac accgccgccg agcgggcaag cgtcgcaaat cccgccctcg ccacagaccg
     1321 aatcctcgcc gttgcaagcc ataccctcgt tcggcccgcc aaagcaggag cgatcctcga
     1381 gcacacaacc acaggcgaac aagccgaggc ctgcctggtt ggccagtggc gcgaactggt
     1441 cgacgaactc gcaggtctct gcgtccggct tcatcgactc ccggcgcacc tccgaggggt
     1501 tgctttcgcc gttgtcccag accgagcagt agacgaaggt gcgatcctct ttgttcgggc
     1561 tgtcgaagcg taggatgtcg tcctcgctaa accgctggta gagcggatcg gcgtaatcga
     1621 aactgacgta atctgcctcg cgatcgggac gtgtgcaggg gtcctcgttc ggcgccgtcg
     1681 gattgctgct cgcgtcgcaa acttcgttcg gcgggtacca aactcggaaa tctttaccga
     1741 accggtgagt gtgggtgctc agcgtcatga ctcggccgta ctgtggaatc tcgaagctcg
     1801 cacaggcctc gccggatgag taagcctcga tacgtcccat tccaaagatg tcgtcggcgt
     1861 cgaagatctg cgtgcgtggg tagagccgtt caccggacgg cgcgaaggtc aggtttatcc
     1921 actgctcgac cgtcgtgtcc gccgtggtga ggttgaacgc gtggctgtcc cagacgacga
     1981 accctttgac gggaaccatc gagaacacgt tgggtggaaa ggcctcccgg aagctcgact
     2041 cctgggccgt cgccaagttc tgccgaacgg tcgtgcgtcc aaagaagcct gcaatcgtac
     2101 ccagctcctg gggcgcatta cggtagttga tgcatgcgat cgtggtgagt ggctcggtcg
     2161 cacattggcc gcggggccca cattcgtcgc tcccgggcgt gcatgaggtg ccggcgaggt
     2221 ctccgcccaa gcactgccaa ttcttccaaa ccgcattcgt cgggtcgagg tgatccgggt
     2281 tgaggacgca gaccgcgccg tctccacaaa ccgactcgtc ttgatcgcag ccgtcgccgt
     2341 cgttgtcccc acccacacag acgcgcggcg gcacgtagaa ctcgatgatg ctgtggtgag
     2401 actgcgggtc ctgtgcgagg agcacttcgt cgtagacgaa gcagtcctgc tccgggccgc
     2461 cctgcgcctc gccgcacggg acggtgaact cggcaggaat ctgatcggag tagtcgtagt
     2521 acgtgatgaa gcagacctcc ccttcaccct cggccggaac ggcccagccg cccgagtaga
     2581 actgcacgcc ctcatcggtt gccggtgccg gtaggggttg aatcttgttg ggagcaaggt
     2641 cgcctgcgag ctggcaagag gcatattgct gcgagccctc gacgatgccg gcctccgggg
     2701 ctccaccgcg aatccaggcg cgcaaaagag ccaagtcatc ctcactcaac gcgccgttcc
     2761 cggtcggcat cgcacctccg gagattgggt tcgggagcgc gctcagctcg aagccctcgg
     2821 tcttcgcagc aaccttctgg taaagggcgc tgagatcctg ctcaccgggg aacacacgca
     2881 tgtagtcgcc cgaggtcgct tcgacgttga tcaagttcgc gtaagcgctc tcgggcctca
     2941 gatcgagacc cgcgttggcg gcctcaccgt gacaggcaga ggcggtgcaa ccacggccgt
     3001 cgaagatctg ttgctgaatc tgagcaaagg tgctgtcggc atcgcacttc tgcgctgagt
     3061 cgctcgatgt atcggatcca catgcggcga cggagagcac agcgaaggct gcgaacgcgt
     3121 attgaacggg tttcattaga cactccaaaa cggggtttta agccaaagtt aggggcggat
     3181 tgtttccgat tagtctatcg gtgcaagtcg aaaagtgagt cttgctcagt ttaactctca
     3241 ccgttcacgg tatcatcagc cctcagggtg aaggcggctt cacgaagtac gatcctagag
     3301 ggatgaacat ctcatcatcg gtggttacgc caccgagaag tgggcaggcg tcacagaggc
     3361 cgccttctcc gcaaaccgag tcatcgccat tgcagcgagt gccctcgttc tcacccccca
     3421 ggcagacccg gtctgcggcg gcacagccgc atgaggccag aaagaagtca caggcgggcg
     3481 tgttgggctt ggtcgattcc cgcttcacct cgagcggatt gtcggcgccg ttgtcgtaga
     3541 ccgcgcaagc cttgagggtt cgatcttcta cggaggccga cgagtaatcg ttgggcggct
     3601 cataataagt gtagagcgga tcgtcgtaga gttggctctt gtagtcggga gcagacgatg
     3661 gtggaacgca gccgttggtg ccctggcatg gtgtgttggg cggcaaccag atccggaaga
     3721 gctcgccgcg catgtgcata tgggagctga gggacagtag ctgcgagtac tgcggcaagg
     3781 tccaggtcat gcagacctcg cgcttcgtga acggctccac ctcggtcatc gcgaagatgt
     3841 tgtccgcttc gaatatctga cgtcgcagcc agacgcgctc ttcttcggga gcgaaggtga
     3901 ggtttaccca ctgctcgatc gaggtcgcgt acttggtgag attgaagccg tggctgttcc
     3961 aggaaacgaa gccgcgaagc ggtagcaccg agtacacgcc agcgggcggc aagtcgatct
     4021 gggtcgactc ttgcgctccc gagagctgaa cctgcgtcgc ggtctggccc ccgaccccgc
     4081 cgccgaggcc gaagtccaca ggagcatacg gatagccgac gcaagcaacg cgagtaatcg
     4141 ccggggtcgt gcactggctg cgagggccgc actcgtcggt ggcgccaggc gtacagctct
     4201 ggcctgcttt ctcgccaccg aggcacgccc agggccccca atctcctccg ttgggatcgg
     4261 actcgggcgt gtacacgttg atgatgctgt gatgcgattg gccgtcctgg gcgagctcgt
     4321 tgcggcggaa cgcaaaacac tttcgaccag cgccgaattg ctcgcaatcg acttggaact
     4381 ccggcgggac ctgctcggtg aagtcgtagt agctcgcgaa gcagacttcg tcttcggcct
     4441 cggcgtcgag atcccagcca cccgcataga actggacgcc ttcgttggcg gcgggcggcg
     4501 gcaggggggc gattttattg ggctggggat cgaaggtccc gtcgcagccc agcagctcta
     4561 gcgtttgctt gacgatggtg tcggaaggag cgccggcgcg aatccaaagc cgcatcgctt
     4621 cgagctgatc gatgctcaga ggttcgcctc ccgagggcat gggctgaccg aggctgccga
     4681 ggtcggtgcc ttcggtaccc gccgcaagct tgctatagag caggctcaga tcttggtcgc
     4741 cggggtagac gcgctcgatt gacggttcga tggtgctggg ctggcgcacg agcgaatcga
     4801 acgatgcgtc ggcgcgcaga tcgagccccc cttgcatctg gtctccatga caggcgctgg
     4861 cggtgcagcc cttggcttca aacacggtcg cttgaatggc gtcgtaggtg ctcgcgtagt
     4921 tgcagtcctt cggtgtgctg ccgccgtctg agtccgtacc cccacatgcg gtgaggaaca
     4981 tcgctgagca ggctacgaaa gtaagaaagg ataggacgag tttcggcatt agtttgatca
     5041 atgcgcctct agagatgggc cgtccgcgcg tcaactgact ctgagtcaat tgacgggggg
     5101 caaggattcg cgcaagcctt gcgactccct cggaaggata gaccatgacg ctagagatgc
     5161 tgctccaagc cgcgaattac gcggtcattc cgttctggct gttgctgatt ctggcgcccc
     5221 gctggtcctg gaccgagcgc ttggtgcaca gccccatcgt ccttctgctt ctaacaccga
     5281 tatacgccta catgctcttc ggctacggac cggcccccga gggcatgagc tttagctcgc
     5341 tctatggaat catggtgggg ttctcggccc ctcacgtcgc ggtggctggc tggatccact
     5401 acctgatctt cgaccttttc gtaggcgcct gggaagcacg agacgcgcgt cgccgcggcg
     5461 tgccgcatct cctgctcgtc ccctgtctat tggccacgct gctgatcggc ccggtcggcc
     5521 tcctgctcta tgtcctggtg aggtttttca gcaagcgtct gctcgagttc gacgaggcgg
     5581 gcgtgcagtg agcgttcgga gcctgaatta gaaagggcca gcgccatgat aggcgccggc
     5641 cctcgttgag taggactccc accaaggggg ggagttggcc taggggctaa ctcgcccaca
     5701 cctccggtga gagtccgatt ctagaattgc agtccatacg catattcctc ccccggcgat
     5761 gccggcgctc ttgggtctct gagcaccatg ctcagaccac gcggagcgtt ccttggtggt
     5821 tagacccacg gatatcgagg gccgtttcac ggcaacgagt gtcctttcgg acttcgcacc
     5881 aatgcggggc aatctgccca accgccgccc gcggcaagcc gcattgcaaa aaggagcttc
     5941 ctttttgggg caatgccact cacccctcga agataacgcc aaggatcaac cgcgccaagt
     6001 ccagaacgag atcaaaggag aaattcgtct tccagctgga gaatctggag gttccttccg
     6061 cgtacacccg ccagctcgcg gaggaccttt gcctcgaatg tcggcttgat gtggtagagc
     6121 agaatcggca gctcttcgct gagctcgagc ttgctgagct cggtcgaaag ggtgtccgga
     6181 gtgaggtggc gagactggaa cgcgagctcg gactcgtcgt ttggaaacga cacctccatc
     6241 atgagcgcct gcaagtcatc gagcgcgttg attcgtgtcc agagctcgtc ggtcggtccg
     6301 gtgtctccac cgtacacgat gctgctggca ttctgacgaa tgatgaatgc agaggtgtcg
     6361 acggtgtgat tgaccatgac cggtgtgacc tcgtatccat ttacctcggc gacccgattg
     6421 ggatgcactt cgacgaagcg cacggtcgga ccgtccttgg cgtcgatctt ggtgaaatcc
     6481 ggccagatca agtcgttgaa gaaatggatt cgaagagctt cgatggtcgc cggaactccg
     6541 acgatgtcga gcgtcgagcc gccttgctgg caacggttgt cggcaagcgt ggcgaggtca
     6601 cgaatgtgat ccatgtgcgg atgactgacc agcaccgagt cgatgcgtgc ttgctcgtct
     6661 agggagagcc cgctggtgat ggccccggca tcgatggcca gggtgtcgcc gatgagaaag
     6721 ctcgaagtac gatgcttcgg tgtctcgcct ccgtggcaac caagaacccg caactgcatc
     6781 aggattctcc cgaacttcga atgaactcga ggaactcgcc aagacgcacg gtgtctttga
     6841 tcagcacgaa gccgctcgag acttcgagaa tgccaacacg agtgagccgg cgcaatgcgc
     6901 tgcggacgtc gttcttgtcg atgccgagct tgagcgcgat gtctttgtcg gtgacgtccg
     6961 gcgcagcggc gccttcggca ttttggagtg tttcttgaag ctcgagaatt acccgagccg
     7021 tgacgtcgtc ttcgagcaga agatcgatga accggttcgc gttttcgagc cgctctgcca
     7081 ggtggcgaat cagccggatc gcgacctcgc ccgttccgct caccatctct tgaagcttgt
     7141 cggccggaac tctcaaaagc tccacgtcct cgagcacctc gatggttgct gtcctcggcc
     7201 ggccggtgac gacggctatc tctccgaaga agtcgcccgg cccgagggtg gtcaccacac
     7261 gttgacggcc ctgcgtgtcc ttcaaaacgc ggaccttgcc cgcgcgaatc acatacatcg
     7321 atccccctgg atcgccctgg cgaaacaccg ttgcgcccct cgaaagacga actccaaatt
     7381 tgtcagccaa agactgatct gtcatgcccc gcctctttca atgtaccccg ctgcgaagtc
     7441 gaccgccagg ctgttacacc gtactgtctt cgaggggctt tgatgtcgtt cgactggcgg
     7501 caagtacagg acatcgcgcc ggagctgagc gtgattctcg acctcgtgat cgcggcgggc
     7561 gcctcttcgc acgcgatttt gcacaagacc gacacgcggt ctgcgaccgc ctgggtaggg
     7621 ctgatctggc tgctcccgtt cgtcggcgcc atgctctacc tctttctcgg catcaatcgc
     7681 atccagcggc gagcaaaaga gctgaagtcc ggcgtcgtgg agtacagcgt gcacctggac
     7741 caagctccac gatccccggc tgacttggac cgcgagctcg gaagcgagcg cgcccggctc
     7801 gtcgagctcg ccagggtggt gggtcaggtg acgaatcgac cgctcctggt cggcaatcag
     7861 ctcgaccgcc tggtcgatgg agacgaggga tacccggcga tgctcgaggc gatcgccgat
     7921 gcacgcacct cgatttctct gatgagctac atcttcgaag cggacagcgt cggcggagaa
     7981 ttcatcgatg cgctcgccga tgcttcagcg cgcggggtcg aggttcgtgt gctgatcgac
     8041 gacgtcggaa cccggtatgg gcgaccacgc atccatcgcg tcctggccaa gcgccgggtg
     8101 cccgtcgcga agtttcttcc ggcgtttctg ccgcgctcga tgacgcactt caacttgaga
     8161 aaccactgca agattctcgt agtcgatgga cgcgtggggt tcaccggagg catgaacatt
     8221 cgacggcatt cgatgatccg cgccgatccc aaggacccga ccaaggacct gcacttccga
     8281 gttcagggcc cagtggtcgc gcagcttcag ggggcgttcg ctcgggattg ggcgttcacg
     8341 acgggagagc atctcgccgg gaagcgctgg ttcccgaagc tcgcgcctat cggcgaaacc
     8401 ctggcgcggg gcatcaccga tggccctgac caagatatcg aaaaactcca atgggtcttc
     8461 ttggctgcgg tgggcgcggc tcggcggtcg atccgaatca tgacccccta cttcctgccc
     8521 gacaccgtgc tcgccaaggc gcttcatctc gcggcaaagc gtggtgtcga agtcgacatc
     8581 atcgtgccgg aggtcgggaa tctgccggtg gttcgctggg cgatgtgggg aaactatcga
     8641 cagattttgg aagaaggcat gcgcatctgg ttatcgccgc ctcccttcga tcattcgaag
     8701 atgtttctgg tcgatggata ctggtcgtcg atcggctcgg cgaactggga tccgcggagc
     8761 ctacgcctca atttcgaatt caatctcgag tgctacgacg aggatttcgg tgccagcctg
     8821 gaagagcact ttcgcgatcg cctacgcacc gtgcgtcttc tcacgctgga agacctcgat
     8881 gcccggtcga cgttcaagaa acttcgggac gggacagcac gcctgatgac accctacctc
     8941 tgagctcgcg cgagctcggc tagacagcgc gccgtcgccg gcgctgcgct ttcgtcgacc
     9001 gaaagatgag cccgaagcag agacagatcc cgcggctcgt cgacgtcgta ccagggggca
     9061 atccgcgcga ttcgatgaga agcgttcgcc tctgccgtat cgcgaagcgc atggacggtc
     9121 gaccagcgaa ccccttcgaa gctcggtcga atgctacggg tcgcgcccag ggcatagtag
     9181 ccgccgtcat tcgccgggcc gaggacgagg ggagcgttct cgagtgcctc gaaggcagaa
     9241 gcgatgagct cgatcggaag cgtaggcaga tcgctgccga taatcacgat tcgttcgtag
     9301 cgctgaagac cttcctcgaa cgcagcggcc agtttgacgc cgagattccc ttggggctgg
     9361 aagcgaaccg tggtgccgag gcggctcgcc cagctcgaaa cggtgtcgtc gtgcgggccc
     9421 gtcgaccaga gctcgatgtc gacccggccc gccgcctgca cgcgttcgca gagtcgcagc
     9481 gtgtcgtcga cgaacgcagc atagagagca gcggccccgt gcgcgccaag gagcccggca
     9541 agcctagtct ttgcaatgcc ttccatcggg ggccgcacga agaccgcaag ctgcgctagc
     9601 gccatgcgta tcgacctagc agccagagga tcttcgtgcc cgcacccacc gaaccgcgaa
     9661 tggtgccact gactttggaa accccgattc gagatcggta ggagaccgga acctcgcgat
     9721 aggaaacccc gagcttggcg gccttgatct gcatctcgac ggtccatccg tagttgcggt
     9781 cttgcatgcg aagcgatcgc aagacgtccc agcgaatcgc gcgaaatggg ccgaggtccg
     9841 tgtactcgac gccgtacaag gcccggagcg cgccgcacgc aatggcgtta cccacttgct
     9901 gctgcgggct gagcgccccg cgagcgcgtc gcccgatcgc acgcgaaccg atgacaatcc
     9961 cggcctcgcc acggagaatc ggctggacca aggacggaag ctcctcggga tgatcgctat
    10021 agtcaccgtc gagaaagacg atgatttcgg gcggatcggc agccagatga gacatggctc
    10081 tcaggcacgc tgcaccgtag ccgcgttcgg gctcgtacac gacctcggcc ccgtgttctc
    10141 gggcgacggc ggccgtacgg tcacgcgaac cgttgtccgc gacgacgatc cttcgaacaa
    10201 gcgacttggg catggctcgt agaacgaggg caatcccctg ctcctcgtcg agagcaggaa
    10261 tgatcacgtc tacacgggcc aatggcgaag actagaggct cttcgagtag tactcgatca
    10321 cgaggtcgat ctcgaccgcg aagggaacgg attccacctt cgggagaccc ttgacggtgg
    10381 cggccttctt gccgtcgtcg aactcgatcc attccggtcg cgcgaggcgc acgtcctcga
    10441 gagagctgtc cacggcggtc aggttcgcgc tccgctcgcg gagggtgacg atgtcgcctt
    10501 cacggacgcg atacgacgga atgtcgagtc gctttccatt gactaggaag tgcccgtggc
    10561 agacgagctg ccgcgctgcc ggaatcgtgg gagcaaagcc cgcgcggaac acgaagttgt
    10621 cgagccgacg ctccagtaac tcgaggagct tgtcaccgga cgggtccttg ctgtgcaccg
    10681 cttcgcggta gagccgccgg aactgacctt cgttgagacc gtagttgaag cgcagcttct
    10741 gcttctcgag aagctgcttc ttgaagtcgc tcatcttgcg gcgtcgagct gacttaggcc
    10801 cgtgaacgcc ggggggatac ggacgatccc acatgctctt gcgcgagagg ccgggcaggt
    10861 cgatgccgag cgctcgcatg cgctttaccc gtggtcctgt gtatcgagac atctataagg
    10921 ctcctggcgc ggaatccctg ggcttttgcc cggcccgcgc gaggcggccc ttatacggca
    10981 atctacgggc gaggcaaatt gagcggaaga ggaggagcag cctttcgggc ggccggctag
    11041 ccgatcggtg aatttccctg taatcaagcc ggatggctgg gcctcggacc attcatttcg
    11101 tctctctggg atgcgcgaag aatcgcgtcg acaccgaggt catgctcggg gttagcgacc
    11161 tcacggggta ccagatcgtc gaggaccccg cggcggcgac ggtgatcgtg atcaacactt
    11221 gcgggttcat cgggccggcc aaggaggagt cggtcgaaac cattctcgag atgagtgccc
    11281 acaaggggag cggcacctgc gagaagctgg tggtagccgg ctgcctctcc caacgttacc
    11341 cggaggagct cgcaagcgag cttcccgagg tagaccattt cctcggctcg agcgacatgt
    11401 tgaagctgaa gaaagtgctc ggcggcaccg cggagcgaat gctcgtcgga aacccggcgc
    11461 aatacaccat gcgtgccgaa gacccacggg tgctgagcgg tcaaacccat cgtgcctacg
    11521 tgaaaatcgc cgagggctgc aaccgtgaat gcagcttctg cgcgattcca tcgtttcgtg
    11581 gcaaacaacg ctcacgcccg attgcggaca tcgtgcgcga ggccgagcaa ctcgccgccg
    11641 caggcgtggt ggagctcaac ctgatcagcc aggataccat cgcctacggc cgtgacctgc
    11701 cgcagcggga aacgctggcg gacttgctga cagccctggg cgacgtgaac ggagtccact
    11761 gggtgagggc acactacctc tatccggaga agctgaccga ctccttggtg gaggtgttcg
    11821 cgcagcacga gaaggtcctc ccttacatcg acatgccgct tcaacatgcg tccgacaaaa
    11881 tgctgcgcgt gatgcgccgc gggcatggtg gcgagcggat gtacaaagtg gtggagcgcc
    11941 tgcgcagggc cattcccgac ctggtgtttc gaaccacatt catcgtcggg catccgggtg
    12001 aaaccgacga ggactttgcc gagcttcgga gcttcgtcga atgggcggaa ttcgatcacg
    12061 taggggtgtt cctgtactcc ccggaagagg gcaccccgag cggagagatg aacgacgcgg
    12121 tcgagctgtc gatcggggag gcgcgccgcg aggaattgat gggcgttcag cgtcccatca
    12181 gccgggatcg tctggagctc cggctcggca ccgagctcga ggtattggtc gaaggcgtga
    12241 gcgacgaaag cgacttcctg ctccaggggc gctggtgggg ccaggctcct gaagtcgatg
    12301 gcagcgtgta tttggcaaac gggaccgccg aggccgggga gtttcgcaga gttctggtga
    12361 ccgatgcggc cgactacgac ctgatggccg acttcgtcga tgcctccggg gctcgagatg
    12421 ccccgccgga cgcaaaagat ccacggcctg ccaaagttaa gctacgaacg ctaacgtgag
    12481 gtggactggg ctcgctctcg tgctcgtgtc gctcgtcgcg gccggtggct gtggggtcgc
    12541 agttccctcc atgagcggtg cgtcgacggt ccccaagaac agaacggact tcggactcgg
    12601 cggcgcggcg cgcgtgccac tgggtgcact aaaggacccg gccgtgctcg atcccggcga
    12661 gtcgcagtat cggtacgcga cggacgctgg cggagtggtt ccgatggcgt acggacgtta
    12721 cgggatcgcg cgaaattggg acctcggact gatggtctcg ggagcgactg tgcgtttgga
    12781 tgcacgctac gaacgggtgc ttcgcgaagg gaccacacgg tcggcgctcg tcgtgggtat
    12841 ggcgccgtac ggtggctgga ttccggatcg cgaccaatcg ggctccgggg gacgagtcgg
    12901 attcgaggtt ccattcgtat acggcgtcga aatcgggggc gtgtacgagc tctggttggg
    12961 tgcgcgcggt agcggagagt acgtcttcgg cgatttccaa gtccaagggt ccgaacaaag
    13021 agcgagtggc gcgggcctcc gcatcggccc ggtcgtcggc atggcgctcg gcgtccgccg
    13081 cgtccatgcg ctcatcgagt tgaccgccgc gtacgaatac tggttcatcg atcacgcggg
    13141 cgcctcgctc gaccgcggcg gcttcgtgct gattcctggc tttggaattc gtttgcggct
    13201 ttgacgagct aggcagcctt cgggcggttc gagcgttgct tcttaggggc cggcttcgcg
    13261 tgatcgccgc gcagaaggtt gccgacgaag gctcccatca gagccatcat ggtggccatc
    13321 aggattccaa gcaggtaggc gccggccgag agctggtaga cgtccgcgat actcgagagg
    13381 aagatcatcg ttggaatcgc cgccagcaaa cctccgacgg gtggctcgag gaagttcttt
    13441 cccggcgaga cgtagttgat ggccgcggcg cccacgaacc agatcaggat cgtgatgacg
    13501 atgcccggga agccctgtgg atcgtaggca gagatgacga ggggtagaag ggcaagagcg
    13561 agcgcggcgg tcgaaaggaa gaccccaaaa gagatgaaga cccagcggag ctcgaaggcg
    13621 tcgggctggt ggcgccgcgc gtagagctcc tcttcactga gctcgtgcga tacatcggaa
    13681 agcctcgccc cacaggactg gcagcgctcg gaacccttgg gattgccggc accgcacacg
    13741 tggcattcga tctcttcggc agccatatct ccctcgctcg acggttcgtc gttccccgag
    13801 gcgcattgtc tacgccggac tggcggagtg caactgctcg tacattcgtg ggaatcgccg
    13861 cgtacttgac gacggtggcc tggcggctac cttgccgggc tgccccaaaa aactgggctg
    13921 aaggaggcct gaagtggccc gttttatcga cgagctcaag cgtacgcatt cctgtggtga
    13981 actgagagag gagcacatcg atcgggagat cgtcctcttc ggctgggtgc agaaccgacg
    14041 cgaccatggc ggttgcatct tcatcgacct gcgtgatcgc gacgggctga ctcaagtcgt
    14101 cttcgacccg gagacgaccg acaagattgc attcgagacc gccaacaagg cgcgctcgga
    14161 gtgggtgctc ggaattcggg gcaaggttcg cgaccggggc accaaggacg acggcagcag
    14221 tcttcacaac ccgcggctcg agacgggcgc catcgaggtc atcgcgctcg aggcgacgat
    14281 cttcaacaag gcggagacgc cgccgttcga gatcgaggac aagatcacca ccggggaaga
    14341 caagcgcctg gagtatcgct atctcgacct caggcgcccg gttctgcaga agaacctgat
    14401 catgcggagc aagctcaacc acgagacccg caactacttc aacgagagcg gcttcctcga
    14461 aatcgagacg ccattcatgg ttcggtacac tccggggggc gcgcggaact tcctggttcc
    14521 ttcgaggctc tacggagggc atttctacgc actcgccgaa agcccgcagc tctacaagca
    14581 gctcctgatg tgcgccggct acgacaagta cttccagatc gtgcgctgct tccgggatga
    14641 agaccttcgc atcgatcggc agccggagtt tacccaggtc gacgtggaga tgtcgttcat
    14701 caatcaggac gacctcttca aggtgctgga aggcttggtg tttcgacttt ggaaagaggt
    14761 ccttggaatc gacctactcg agcgctaccc gagtggtgaa ttccctcgac ttccctttgc
    14821 agaatcgatg gatcgcttcg gcaacgacaa gccggacatg cgcttcggca tggagcacat
    14881 cgatctgacc gacctcacga tcgaacacgg tgggggcgga atcggcatgc tcgagccgat
    14941 tgcgaagaag ttcagcgaag gcgcgtaccg gcgcgacctt ccgatggaga tcgtgaaagc
    15001 gatgcgggtc ccagccgggc acggattctc gcgaaaggat ctcgacgctc tcgagaagta
    15061 tgtcggtcag atgggtgcca aggggctggc gagggcaaag ctcgacgccg agggcaactg
    15121 gctgcaaagc ccgcttgcca agatggtgac gcccgagttt cgcaaggcgg tgaacgagaa
    15181 ggttggcgcc gaagacggcg acctgatctt ctttcagttt ggaccgacgc agaaggtcca
    15241 cacggtgatg gcgaatctac ggctacacct cggacgcaag ctcggtctga ttcccgagac
    15301 gggtagctcc ggcgattgga actttctctg ggtggtcgac ccgcccttgt tcgagcgcaa
    15361 cgaggaaaag aagcgatggg aggccgcgca ccacgtattc acgcgtccgc acgacgaatg
    15421 catcgacctc ctcgggaccg accccggcaa cgtgctttgc tatcgatacg acctggtgct
    15481 caacggcttc gagatcgccg gcggctccat ccgtctgcac gatcccgaag tgcagaagaa
    15541 ggtcttcgag gtcattggac tcagtgacga ggaagcccga gagaagttcg gatttctgct
    15601 cgacgcgctc acctatggcg cccctcccca tggaggaatc gcatttggaa tggatcgagt
    15661 cgccatgctc gctgccgaga ccgagtcgat ccgcgatgtg atcgccttcc cgaagaccca
    15721 gcgcgccaac gacctgatga cctccgctcc ctcccgggtg gatccggggc agctcctcga
    15781 gctcaagatc aagactttgg agtaggtcct ccaagcactg acgggacgcc gtcccctcga
    15841 aaagcaccat tttcgcgtct gaatatccgg aacttgcccg gcgtcttcaa tcgctgacaa
    15901 tgtggttcat catgaaagcc ctcctcaact ttgccctcgt tgccttgtgt gtgctcggga
    15961 tgaccttccc cgaggtcgga cacgcggatg agccgaacga catcaagaac tgctacagcg
    16021 tccacgggga agcccgttac gggccgctgg cgtacaagca cgtcgtcatc gtcacgaacc
    16081 gatgcgacct gagtttggcc tgcgaagtct ggaccgacgt agacccgacg cccaagcaat
    16141 cgctcacggt ggagcccaag ggcacgggtg aggtgatcgt gcgcacgagc tctcctgcca
    16201 gggctttcaa agcctttggc gagtgcaaaa agcagtgaca ccgctcagta gagcgggttg
    16261 ttctcggcgt tcttgaagaa gcgttcgaag atcgcgtaac cctcgaccag cttgtcgagc
    16321 tcggtgtact cgttgggctg gtgtgcctga gctgcggttc cgggaccgag attcacgcca
    16381 gccacgccga tggcgtcgaa gcgcgcaaca tcggtccagg cctgcttgct gcgaacgccg
    16441 ttgacgccgc agctcatcag atactggatg taccgatcgt tcgcgtgtgg atgtcctgcg
    16501 ggcgagaggt cggttgcctc gaccttgcat tgatttccgg ccaggtcgcg cagctccgcg
    16561 aaggcctgtt ccggcgttcg gcccggtgca aagcgatagt tcacgttgag ctgggcctca
    16621 cccggaacga cgtttcgtgg accaccgcct tgcaaaaccg tcggagtcat cacctcgcga
    16681 aacagatacc cgtcgaggtc gacgtcatgc ggcgcccgct tctcgagctc agccaagaat
    16741 cgcgccgcct tgtagagcgc gttgtcaccc tgccaggggc gcgcgctgtg cgccgtgcgg
    16801 ccttggaagg tgagtgttgc atggatggag cccatcgcac cgagctgcat ctgattgtcg
    16861 cttggctcga ggcagatcgc gaggtcgagc tcgtgcagct tcggatagtt gtccagaatc
    16921 ggaccgagac ggttttcctc gaagggccct tcctcgcgct cgtagaagat gagggtgagg
    16981 tcgcaaggga gcgctcggag atcgagacgc tcgacgagct cgatcatgat cgccaagccg
    17041 gatttcatgt cagcagcgcc cgctccgtag aggagattgc cctcgacccg gggcggtccg
    17101 tcgtgctgcg tgcgcacggt atcgaggtgc ccgaccagtg ctatgcgtgc agcgttctca
    17161 cgtttggcgg cgtgcacgac caagctgtcg tgaaagcgcg tgatcgcgcc ggcccccagg
    17221 cttcctttca gccgatgctc gacatagtcg cagagctgcc cttcctctcc tatcgggctc
    17281 ggaatccgtg tgagggcgag caaggtgtcg cgaagcgcgt gcgcgatttc ggtcatccgc
    17341 gccaagccta gcaggcctag cgagagaggc ttatcgaggg aggtcctcgg gggtgtccca
    17401 gtcctcgaga gcgcgcgtga tccccgaagt caggggcaaa gcttcgacct cgagcagcgc
    17461 gaagagctgc tgaaacgaac ggagacctcc ggcgatggcc tcgtcgagga caggggcgag
    17521 tcgagcggta tcgtagcgag cgagcatcgg ctcccagggc gaatggtgcc cgcggcgcgc
    17581 cgccagcacc ggggcgggac tcgggtgcgc ggcgatgtag gctagcgttc gagaagtgac
    17641 gtacggcatg tcgcaggcaa cgacgatcag tcgtgagaat cccgactggg acgcatggcg
    17701 aacggcggcc tgcaagcctg caagcgggcc cgcgcctagg gggtcgtcat cgatccgagg
    17761 cacccctgcg gcgagctgcg catagggcat cgcgtcaccc acgagcaccg gctcgaatcc
    17821 cgcctgccgg ccagagcgaa cgagcgcgtc gatgatgggc tcggacgccc cgggcagctc
    17881 taggagcccc ttgggcgtac ccatccgact ggactttccg ccgacgaaga tcgcaagcaa
    17941 cacgtatgcg tcgatcgaca actagaccgg cgctccgagc tcgtttgtaa ccacagccgg
    18001 catcttctcg acgatgcggt tgatctggtc ttgctggatc gcgctcgtta cgaagttcgt
    18061 ccagaacgcc gtgaagtagg ccgcgacgtc gagaatgact gcgatgatcg tggtgtcctg
    18121 aacgggtccc agcccgttgc gctcctcgaa ctctgcgatc tgctgcgaga tccgatagtc
    18181 gacccagatt ccgtagatgg acaaggtgag aatcacgaag aagaaatcga gccaaaaacc
    18241 atgaccggtg ggagtccgac cgtcgagcgc ctcgagatct tcgtagatac cgtgatacca
    18301 aaacgggaga tagatcccga aggtgatgat actgagaacc aacaccagag ccggctcgcg
    18361 gcgctcgatg attggagctg tcatcgcatg gtcccttccc gctcgcgcct tgcgctgcgt
    18421 cggctaagtt tcgtcccatg gacgaaggcg ccacgcaagc ggccaccatc gctgtgatcg
    18481 gcgatgttca cctgttttgg gacgacgagg acgtcgcatt cttcaatggc gctggctacg
    18541 acctcgtgct cttcgtaggt gatttggcgg gatacactca ggtaagaggt cgtcgtgtcg
    18601 cgcgctcgct gagaaagctc cgcgtgcctg cgatgtgcat tccagggaac cacgacggtc
    18661 tgcacgcgtt tcagctcggc gcggagatct ctccccgcgc acgcccgctg cgaaatgcct
    18721 tctgccgggg gcaggcggct cgctgtcgaa acctcgaccg cgcgctcgga gaggtggagc
    18781 tgctcgcata cagcaggcgt cgattggcgc cggctggtgt ccccttgaac ctggtggtcg
    18841 gtcgcccgca ttcgatcgga ggtcgacgtc tcgcttgcat gcggtatctc gcagaggagt
    18901 acggcatcga ctcgatggac gcctcgatcc ggcgcttgca atcgttggtc gattcttgcg
    18961 acgaagcccc gatcctcttt ctcgcccaca acggcccgtc gggactgggc gatcgttcgg
    19021 attcgatctg gggctgtgac tttcgcaaga aagaagagga ctggggagat cgggatctcg
    19081 aagaggccgt ccggtatgct cgtggcaagg gccgcaaggt gctcgccacg gtcgccggcc
    19141 acatgcaccg ccgcaccaag tccggaaagc gtcggcccgc acaagtcgag aaggacagcg
    19201 tgctctacgt aaacgccgca gaggtgccgc ggcaccgcaa tgaaaacggc aagaagacgc
    19261 gccaccacgt gaggctccga gtgagcgccg aacgtgcggt cgcggaggat ctctggat
//
LOCUS       DASRZF010000307        31098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2299399, whole genome shotgun sequence.
ACCESSION   DASRZF010000307 DASRZF010000000
VERSION     DASRZF010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 31098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31098
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2299399"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="VFG22_13395"
     CDS             <1..611
                     /locus_tag="VFG22_13395"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET6417285.1"
                     /translation="RVRERWQMVGAHIASAFRLRQGLKTLAADSRRAHTDLPRNAEAV
                     LDLEDFRIVGQAGRAEGNRVLKTLRGAARSVDQARGALRGDDPQKALEIWKALIGGRW
                     TMVDWFDTDGRRFVLAIPNSPELGEPRGLTEQESQVVNYLLLGDTSKVVAYRLGISSS
                     RVSGLLKSAMRKLGVSTKAQMIEKLRPFVPQETPEGDGSAAV"
     gene            complement(608..1402)
                     /locus_tag="VFG22_13400"
     CDS             complement(608..1402)
                     /locus_tag="VFG22_13400"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6417286.1"
                     /translation="MGTMGMKRILITGAAKGIGSATVRLAIEAGHQVVAVDIDREALV
                     RLREELPGIETAILDVRDLAAWERVVESAERQGGPIDILVNNAGVCLPGPCNEVSAED
                     DRLTVEVNLLGVMNGVRTILPRFLERQRGHVINVASMAAFAPAPDLAAYCATKHAVRA
                     YTHSCALDLRHAPLDWTLVCPGAVDTPMLEGMRKRRAGVVVFTEKAMAPEAIGRAIVE
                     AIREPRREVLVPSVRGKLIRFLGLFPGLLSRGMDDAERRGRAALGD"
     gene            complement(1384..2253)
                     /locus_tag="VFG22_13405"
     CDS             complement(1384..2253)
                     /locus_tag="VFG22_13405"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6417287.1"
                     /translation="MGEKDKHERVAIVTGASSGIGEATAHCLAKAGFKVAVAARRADL
                     LDALVSAIQADGGEAFSVPTDLSDAEQTSALLTKTLERFGRVDVLVNNAGYGPPYALE
                     QMNREALRHVFDVNLLSGMQLIAELTPTMRKQGGGRIVNISSLSRYVGAPLAASYAAT
                     KGGMEAMNACLRLELAPWNIKLSVIVPGFVDTPTFETARAAGAHLRDDPENPYRQLMS
                     HLDDFATEQLKSAVAPEEVGKAVVKAATAESPKTRYFIPRSARTAARMFGALPDGVAD
                     RLLLKMYKWGPWA"
     gene            complement(2246..3511)
                     /locus_tag="VFG22_13410"
     CDS             complement(2246..3511)
                     /locus_tag="VFG22_13410"
                     /inference="COORDINATES: protein motif:HMM:NF018452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HET6417288.1"
                     /translation="MSAKNPSISQSAWDNFAEELKKLGDKLVGPTGARSARERAEGFR
                     YLIRLIGAAQELELEADRQRPSLNRMFTPTRKFIAEGTDTLYHEAKLDPQRSYEMTVR
                     RGDDLFFSVVVYAEDDDGDKLIVDDLNDEGIVFDEQDGARVAKIHLSAKRPDGATNWL
                     KLEGRRPFVLTRQYFPDFVTEVDAGQYRQAVMEIENIDHPAAPPELDEDHLARGLDRM
                     VAFMNETLDSALGISAFVGLNTIEYERTDTSPTRIGSDGNLVLDHESHEEYSPDELAE
                     MVDPRVVANNLPGPGIGYVGAQFKLADDEAILIEGKDVPCRYWSAQILTKFLESGDYR
                     HHRVAINNRQVELDDDGTFRIYASETNPGVRNWMSTQGYRRGQIVLRTLLAEEDMNAE
                     LSVVKIAEIPERDRAAQATVARQTRSTNG"
     gene            complement(3556..4587)
                     /locus_tag="VFG22_13415"
     CDS             complement(3556..4587)
                     /locus_tag="VFG22_13415"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET6417289.1"
                     /translation="MDPTSSAIIDVTEAAYDLAVSREEWLPELLRRGLPMLDQGLGVA
                     GYEYARPPDQGDVEITRFHVASGPEDFAERHLRALATTEPDVLRDQLRPIAATTGSEY
                     AKVTNRPQDLEHYVSHVGYGEDVLFLTAVDLHGAGVAIVAPLAEITALSRQDSERWQM
                     LTAHVETGHRLRRALSTPATAEEGGTELPLAAEAIFDARSFRLTDAVGPAQQSGATRK
                     LRDAAIRVDRARGKLRRDDPDEALQIWKAMVRGRWSMVDWFDSDERRYVLAVPNQPSV
                     TDPRGLTERENQVVGAAVLGHSNKMIAYRLGLSTSRVSTLLRSAMRKLGFRTRNELVS
                     HMSDFQALD"
     gene            4837..6183
                     /locus_tag="VFG22_13420"
     CDS             4837..6183
                     /locus_tag="VFG22_13420"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET6417290.1"
                     /translation="MNARVPRICIIGAGISGIAAAKCLKEKGLAYDCFEASSTLGGNW
                     LFQNPNGMSSAYRSLHIDTSKQKLEFPDFPMPDEYPTFCHHSHIHQYFEDLVDHFGLR
                     DAIRFGAPVRKAERRADGAWKITLDAGVRDYQHLIVATGHHWDPSWPEFPGTFDGPTI
                     HSHQYIDPFDPIDLKGKRVLVVGIGNSAVDIASELSNRGLAERLVLSTRRGAYVVPKY
                     LFGKPIDQIVETNPYIPLAVQRKVAALLIRWLVGRVEDYGMPTPDHELLTAHPTVSSD
                     LLQRVGNGDIIVKPNVAELLGDRVCFTDGTVEVLDAIIYATGYKITFPFFDPELIAAP
                     DNVFPLFKRVFIPGFDNLMFVGFAQAIPSIIGFVQDQAKWIAAYLAGEYALPAIDEME
                     AAIERDEARMMSHYVASSRHTMQVDHVLYARDLAKEWKRGRRRARAGLQHGSAVSG"
     gene            complement(6173..7588)
                     /locus_tag="VFG22_13425"
     CDS             complement(6173..7588)
                     /locus_tag="VFG22_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417291.1"
                     /translation="MTLLCGCYEISYPSEGYQVVENILEAPIVAGTGSKSNFLLAGFP
                     WEVANQVDKARFVFFERLPFSVWECKGIGCATRLQVITDNGAVIPHSRRIGQDMHVCT
                     ADYVQTVGRVHCGERRAGGELLDRPRTSYIGDASDVVWEADYFSRLISGRHSWTQTTA
                     PWQPAEDGVGAEWSETPWTGQPLERSLQSAHNLDLGACSVFVPWKWEDRDPRLDPMLG
                     PMLGDRGLAELTLDELDSAREASASTNEMQDVAWFFDAFTNLWPREIPSEFHYQVTGT
                     GARQVCLRSYFSTNTKLRTRPSGPNIIAESIVAGFTALFGIGDCKTHPMSVRFCGELG
                     TVAGAPRFTLDDSSVRVEMEPYSRFKPSCNNKFVPLVKEGLADGVEKLTDAVMTEKLA
                     ALTSGLPFEIRRIELSPSGAYLVVAEDYHDTQYAAMKLAGVALARDRGVLCRPELGDS
                     DFEHLPIAPYDFTVPKRGITR"
     gene            complement(7681..7752)
                     /locus_tag="VFG22_13430"
     tRNA            complement(7681..7752)
                     /locus_tag="VFG22_13430"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7718..7720),aa:Gly,seq:ccc)
     gene            complement(7743..8006)
                     /locus_tag="VFG22_13435"
     CDS             complement(7743..8006)
                     /locus_tag="VFG22_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417292.1"
                     /translation="MRGPDNNGATAFQNRSINVAFDAHAVVKVEQGRIRAVVEEHGGE
                     QRCERFANHPLRTIVRQTGAPIRGGPCLTAKASGASSCVSLRV"
     gene            8141..10483
                     /locus_tag="VFG22_13440"
     CDS             8141..10483
                     /locus_tag="VFG22_13440"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET6417293.1"
                     /translation="MRELLRATARFGLCLGLLMGLSADPAEAQDLVAPRVKSLPGIEV
                     PEGVEIPKSGVVQALVRIDIDGKGQVQKCNAGRSLCDLVIEGIKQAEFEPAMRDGHPV
                     PSQVRVDLRLRKEQTPQSTRDEALLEPSTQTVEKELIFSETAEVDARVQTPIRFELDG
                     IRNIPGLFGEPFRICEVLPGMLPVANGQPYCYVRGSPPSGTLYVYDSVPIPLLFHSAL
                     GPATVLPGLIGTLDTYSGAAPARYGGFTGGVMAATARELPTDRVHGEAELRLIDVSAL
                     LNVPMPKEGSMTFGGRYGFPNLLLGAVNVDADVNYWDYQYRTGVAMSSKSRFEVSAFG
                     ARDASVFDDADPAARLALDLQFHRVDLRFLGKVNRWDLVGTMLYGYDFSNVDDGSGSA
                     VSDAGASIHRFGPRFWATYGAPKFQVRIGGDVVGLFGPARCTPGIQNVSTPCDPEFAA
                     QDRRVIGGVFVDGNISPISWVDLSLGLRADVWGTAGYRDAAASPRARVTFHPHELVDV
                     FAGWGFGFRPATFAIPLPGLGDVPLEPGLQRANQTVGGIRLNLPHELSFEARGYLNLF
                     RDLRFVDVFMNPEISDDPASGPLPAGALDDSADGRSYGLELLFRRPFGIGFSTLASYT
                     LAYSDLTATALLFDAPPQTFDYTPSYDVRHVMNGALAWQADFGLIISARVFARSGRAE
                     GWLWRDGSGQVQQYVQRVPWFVRLDAEIAYQWAKPGRRMRISLEWINITQARDSQEVD
                     TSDPSAPFECRIRWGVPSGPCPIKFTSAIWYPNLSFRAVF"
     gene            complement(10491..12749)
                     /locus_tag="VFG22_13445"
     CDS             complement(10491..12749)
                     /locus_tag="VFG22_13445"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET6417294.1"
                     /translation="MTETVQTFCRICEALCGLEVTVEGNRVVDIKPDAEHVATRGYGC
                     LKGLKQHRMYDTPDRLLYPMQRIDGELRRVSWDDALGSIGGKVRQLIDEHGRDSIAMY
                     VGTAAGFGVLHPVFAQGFMQGVGSANMFSSATQDCANKFAVARQVYGFPFTQPFPDLD
                     KLECLIIVGANPAISKWSFLQVSNPIRRLKEIEARGAKIFIVDPRRTETAKVAGEHVF
                     IRPNTDVFFYLSFLNELLSTAKIDRARIDRYMTGFDEVCRIAEAWPAERTAEVTRIPA
                     ETLREMVRAYNEADGAALYCSTGVNMGTNGSLAFWLQECINAISGNLDREGGTLVSTG
                     VIDFAAFGKKTGTLLHENESRIGKFQSVNDSMPGGILADEILTPGDRQIKALFVTGGN
                     PLITMANSERLREAFKELELLVVLDIQPSETASVATHVLPCTSPFQRPDLPFVFPLML
                     GLQAKPYLQATRAVVPPQGEQRDEATIYVDIASASGVNLWGSALAQRTFETAKAINSV
                     GRRGEQPSIPQELLLSGLLRATKQGSFKKLLKEKHGRLRPQHEPGSFLGKRVVHDDRK
                     VHLAPSPFLEQAKKLESDFELELRTKDRLKLITKRAITTHNSWTHNIEEFVSGERTTN
                     HLYMHPDDAARAGVGEGSLADVSTATATVRIPVKLLSELMPGTVALPHGWGHQDSKMG
                     VASKTTGVNVNLLAADGPDRLEKVSGMAHLTGFLVDVKPAAGALNPHHWSGIGREATG
                     KAPPVDGVAAES"
     gene            complement(12771..14936)
                     /locus_tag="VFG22_13450"
     CDS             complement(12771..14936)
                     /locus_tag="VFG22_13450"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6417295.1"
                     /translation="MAINRNKVLDAARKYQSRGQYDKAIAQYKKLVDSDKRDVRSLLK
                     IGDLHVRKGNRGAAIETYETVAGHYSTQGFFLKAIAVYKQILKLDPSRLDAQVKLGEM
                     YEQLQLISDAMSVFEDVSTGFMRAGDTDQALAMLGKMVDLDPEHIPVRIKYAEALSRA
                     GRTQEAADEFQQGAVLLRDQGRLDDYIKVAERLLYHRSNDVHVAKELAETYIARRDPK
                     RALAKLQICFKADPRDVSTLSLLAEAFLMLSQTEKAVSVYREIARIHREAHRPQERMS
                     ALHRILELSPNDQEARMALQKTASASGQHYMPSGAMHQPEATGSTSVSAAPLSAATSL
                     SSPSVRSASGLSGASGIMYVEEDVEVEEASTLSGSLPAISEELIEVEDSYASVPPAPR
                     PPSGGRVIPMRTLGPMSYSEFVSLPLVSTQVAAATAAIAPSLMEQTLELADSYLVDGL
                     FNQARAIVSELQLTLPDHPLVLDKAREIEELAAASSGVSAVAQPLIRPSVSSMPATLA
                     SDPPDMELEYEELQYGDEGFDFAEKLAEELAEVAEIAPMDEGAEMIDVETVFEQFKAG
                     VAAQVSDDDSDTHFDLGIAYKEMGLLPDARREFQVAMSDPRRRCLCWTMIGLIYLEEG
                     QPRDAMEAFQSGLESPEKTPFEAVGLHYELGMACEQSGLSDQARLHYDYVFQREPQFR
                     EVGARLQRLGGPSGISDQDLMLESMDDVNRAFDELIHED"
     gene            complement(14983..16347)
                     /locus_tag="VFG22_13455"
     CDS             complement(14983..16347)
                     /locus_tag="VFG22_13455"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HET6417296.1"
                     /translation="MFKKVLIANRGEIALRVIRSCRERGLRTVAVHSDVDADALHTRL
                     ADEAVCIGPADPKRSYLHIPAIIAAAEITGAEAVHPGYGFLSENPEFAETCAQCGLHF
                     IGPLPEQMRQWGDKVSARKLAKSLGLPTLEGTEVVEDVEDAVRKAEGLGFPVLLKAAG
                     GGGGRAMHVLHSREEVRAVFEIARSESRAAFGNDALYFEKYLERPRHIEFQILGDAHG
                     NIHVLGERECSIQRRHQKVLEEAPSVALSDEQRSDMTATIRRAMGQVGYLSAGTLEFL
                     LDERGELTFLEMNPRIQVEHPVTELVMGVDLIDDQFRIADGQVLELPAHPLTPRGHAI
                     ECRINAEDPVTYAPSPGRITELHMAGGTGVRVDSGVYGGGLVPRHYDPLVAKLIVHAP
                     TRAQAIAKMRRALSETIIGGIRTNIPLHQRILAHPAFEEGRYTTRFLEELLPEHMLTS
                     PVRH"
     gene            complement(16351..16800)
                     /gene="accB"
                     /locus_tag="VFG22_13460"
     CDS             complement(16351..16800)
                     /gene="accB"
                     /locus_tag="VFG22_13460"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HET6417297.1"
                     /translation="MKLDLDQLRDLIRILDESNLTEIEVEHDDDRIRVRRDPAPLVGA
                     ISHSVAPAGAASNLPPGSEPAPAESEGDFVTSPFVGTFYRAPSPEADCFVEVGDTVVP
                     GQVLCIVEAMKLMNEIEAELACTIVEVLVEDGKPVEYGDRLFRIEPS"
     gene            complement(16823..17278)
                     /gene="aroQ"
                     /locus_tag="VFG22_13465"
     CDS             complement(16823..17278)
                     /gene="aroQ"
                     /locus_tag="VFG22_13465"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HET6417298.1"
                     /translation="MTAQAKRILVLNGPNLNLLGTREPDVYGSTTLKDIEKAVMDLGK
                     DLGAHVECRQSNQEGQLIDWLHDARDAFAGVVINPGGLTHTSVSLRDGIAATGLPVVE
                     VHISNTQARESFRHQSVTAAVCIGSVIGFGKSSYTLGLRALIDYLNAAG"
     gene            complement(17283..17639)
                     /locus_tag="VFG22_13470"
     CDS             complement(17283..17639)
                     /locus_tag="VFG22_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417299.1"
                     /translation="MFKEILQDLVERADGGVAGLLMASDGIAIDQYSTGDGPFDIEAV
                     GMEYSVVLKGVQRAAEMLDTGSTNEVSVTTERLTTVVRMLTDEYFVALAISPGGNIGK
                     ARYLLRTSAPKLIENL"
     gene            complement(17682..19166)
                     /locus_tag="VFG22_13475"
     CDS             complement(17682..19166)
                     /locus_tag="VFG22_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET6417300.1"
                     /translation="MREADVVVIGAGFGGLATALRLAELGADVVLCETLRYPGGCAST
                     FTRNGYQFEAGATLFSGIGDGQLFSEWNRIWNLGVRFDALDPLVELRTNDFSLKIPSD
                     PTEFLRRFVALPGANPVAVERFFDTQRGVADALWRLFDDPSLLPPLNGSALLRHLHRT
                     HSYAPVVRWMRRSLSDVVAHHGLADWEPLRIYLDAVSQITVQASAQEAEAPFAMAAAD
                     YFFRGTGHVHGGIGSLAWGLVHAIQKAGGQVELANRVKSIVRENGRWRVTTRKGELRS
                     SAVVANVLPQSLSAMSETPFEGSLDDVERSVEDGWGACMLYLAVDAEAVGGDEAHHLQ
                     LVDDRTSPFVEGNHVFCSISAADETARTPNGERTVTASTHVPMKALRTLGAEARAEYV
                     GAIQERMRTTIRRRAPGLLEGARFELTASPRTFERFTGRHEGFVGGVPRRVGLRHYRP
                     RSLWPRRAARGLYLVGDSVLLGQSTLAVALGGVRTAERIARRDT"
     gene            complement(19176..20579)
                     /locus_tag="VFG22_13480"
     CDS             complement(19176..20579)
                     /locus_tag="VFG22_13480"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET6417301.1"
                     /translation="MKLRARLALTLSLATVSLLVLLGWAQSRWHSQLRGEALAEAAVH
                     RMESGERERCEADPARWPGPQSVRRSERRPGPPPRRLPPYRIFAYGADFQSLNPENPP
                     FPPELASRLEAGDDIAASRSPDRRRTEVAVRMPWVDGPCSVVLMRAKGPPKWFSGAVL
                     MPALLVAVVMILVALFAAGPIVGRVRDLTESVRRLGNDSNERIHVRGNDEIAELGAAF
                     EQSRLTIRAQLESLRDRETALRNYIANTTHDVMLPLSVLQGHLVSLQQRIEAGETLDV
                     AAVVPSIEEAQYLGSLLHNLNAMARLESIEGLAHRDSVDLNRLVERVVERHRPIAIRK
                     HIALDSAVPETSIHFRGDLTLLEQALGNIIQNALRYNESNGHVAVVLRGSDDAWDLTV
                     VDDGPGIPEGERKRALERAFRGNQARTRHPHGMGLGLHIASDVARRHGLGLTLRDTDG
                     GGLTVELRHTGQEARRP"
     gene            complement(20576..21244)
                     /locus_tag="VFG22_13485"
     CDS             complement(20576..21244)
                     /locus_tag="VFG22_13485"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6417302.1"
                     /translation="MTNRILLVEDDLALGQQVVETLSRAGFDPHWVQDGDAARQLDPD
                     AYRVIVLDLMLPGTYGMDLLKRFRVKSEVPVLILSARDDTADKIRGLELGADDYVTKP
                     FFPEELIARIKACLRRPNLVSTKLITVGPIRVDLERREVSHGQGVLSLTPVEFEIISL
                     LARRKGSAVTRDALVDAALDPAHEGSRRTLDVHISRLRSKLGEHATRLETVWGIGYRL
                     SEDA"
     gene            complement(21257..21697)
                     /locus_tag="VFG22_13490"
     CDS             complement(21257..21697)
                     /locus_tag="VFG22_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417303.1"
                     /translation="MKLIKKETFVAAILGGVTALALTVGGVALAHGAPHGGPHGQGGP
                     WSPERMEEHLAEMSERLNLSAAQETAVRAIMEDAAARGEEIKEMPRGKEKFVAMKDLR
                     FATEDQIYANLSCEQRDDLRLLQREHKVERMERHFGRGDAEETK"
     gene            21802..22530
                     /locus_tag="VFG22_13495"
     CDS             21802..22530
                     /locus_tag="VFG22_13495"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HET6417304.1"
                     /translation="MARSFLVLDIETVPDADLAWDAAVDGFPPAPFHQVVALGVLWLD
                     GDHSLKKLGAFGGEENAPPDESKVLGEFAEFIGERHPTMVTFNGRRFDLPVLANRMLK
                     HGVPFPAYYAGRSEGPDYRYRYNDEGHMDLADLLTDFGASRMPSLTALAQLVGMPGKM
                     DVDGSKVQSMYEHGRHAEIRDYCLHDVVQTTFVFLRSELLRGRLSKDEYRNRAEALWT
                     ALESDPRALPVLERANQAQALLSL"
     gene            22645..23220
                     /locus_tag="VFG22_13500"
     CDS             22645..23220
                     /locus_tag="VFG22_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417305.1"
                     /translation="MRHLLHFGLFVFFLTAATRADAQDRRRVYPQTRVTVVSTDGLRA
                     AHPGRAYFEERQDLEEITRIARGWYRAAANGNRRAELALDRRLDAWLDREIRESIRTP
                     RDHRYAMQIRALGQQLATLERRPYHAYGHGHGHGHSHGQVSHRETHRSLGGYYERKAM
                     IIDELVELSEQRVRNAYAAIHRPIRMSFAHR"
     gene            complement(23280..23609)
                     /locus_tag="VFG22_13505"
     CDS             complement(23280..23609)
                     /locus_tag="VFG22_13505"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET6417306.1"
                     /translation="MPKTVLTPQNLDAVATRFRVLGVPLRLQILNELAHGERTVTALV
                     EATGSTQPNVSKHLTTLLAHRLVRREQRGNSAYYSVADPAVFELCAIVCGNIERALDE
                     ERRAFSG"
     gene            23709..26327
                     /locus_tag="VFG22_13510"
     CDS             23709..26327
                     /locus_tag="VFG22_13510"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET6417307.1"
                     /translation="MKRKILDFPLQHARLVFVGLAVLTIGLGSAIPWIQVDTDPENML
                     SERDPARVFHNQTKEEFALHDLIVVGVVNEVEPAGVFNPESLARVHELNERLKSIDGV
                     IARDVLGPSTMDNIEQAGPGAIRFEWLMEQPPKTPEEALAIKEAALRLPMLRGTVVSE
                     DGKSIALYVPIESKDQSHRISEEIRTTIAGFGDNTDDFYITGLPVAEDTFGVEMFKQM
                     AVSAPLAALVILLLMWWFFRSFALVLSPMIVALTTVTITMGALIASGFTVHIMSSMIP
                     IFLMPIAVVDSVHLLSEFVDSYAKQRDRKAAIKEVVGHLFAPMLYTSLTSAAGFLSLT
                     FTPIPPVRVFGVFVALGIGIAFVLTILFVPAYIAVLPEKRLAALLSSKRDKKDGGLLG
                     RAVAAVGDKSLRFAKPILVVTVAAIAISAYGITKININDNPVRWFKPSHEIRVADAVL
                     NRELAGTYTAYLIMKQDTDAERQIVEAGDAAVQGASDGVRAKWAELKTESETASREGV
                     TNVSDALVEATSDLVFDTAGEESEQWQSVQTALEKAQSASKTFQNPEHLAYIERLQRA
                     AEALPLVGKTSSIADVVKTVYRELISGEDKDYRIPESNEGVAQTLLQFQSSHRPNDLW
                     HLVSPDYRESAIWMQLKSGDNQDMMSVKAELDAFLSANPPPDGLRAQWAGLTYLNVVW
                     QDEMVGGMLESLMGAFVIVFLMMVILFRSPVYGVLAMIPLTITILFTYGMIGLVGKDY
                     DMPVAVLSSLTLGLSVDFAIHFLERARSIQAVTGDWKETMALMFQEPGRAITRNAIVI
                     AIGFLPLLAAPLVPYNTVGFLMAAIMAVSSIVTVLLLPSLMMQIQNWLSPRKEKVSTN
                     AEDASASLATNKEQTI"
     gene            26324..27133
                     /locus_tag="VFG22_13515"
     CDS             26324..27133
                     /locus_tag="VFG22_13515"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HET6417308.1"
                     /translation="MRTLSKTSLAAIAAAILFSTPTLSAEAGDLKDASEIVNRTNSAQ
                     YYAANDGRALVRMKIVDDKGRSQRRQFAVLRRDVKDGADQNYLVVFDQPADVRNTAFL
                     VKKHLNKDDDRWLYLPGLDLVKRIAASDERTSFVGTHFFYEDISGRHPSEDTHQLLET
                     TDTEYVVKSTPKSSKGVEFAHYTLRIDKKTFLPSKVEYVNAQGKVYRRIETVKVEDMQ
                     GHPTITRLKVSDLDSGGYTLSDVSNVQYDLGIPDDIFVETSLRNPPQKWLR"
     gene            27252..28763
                     /locus_tag="VFG22_13520"
     CDS             27252..28763
                     /locus_tag="VFG22_13520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417309.1"
                     /translation="MALAFASGAFAQGPRDGAPPVGDEASGEPEEEPASSSRSEPPLD
                     PSSEEALEAGAEGPVDAAKTTKTPEELAGDVEPSDEAGPDEVWDEFDDWEEEKGDDSG
                     GKAWDFQIGGFVEGLIAPRVVQSSASENEFVANEARFRLNLDAVHEIASARFRGDVYA
                     DAVTRTIWFDIRDASVFLRGGNWFNMRLGRQVLTWGTGDFVFLNDLFPKDFNSFFIGR
                     ADEYLKAPSNSIITTFNIKKVGLDLVWTPIFEPDRFIDGERLSFFNPLLGEITGDRSD
                     FTPIDPVYPSQRLKNGTFHGRLYGTAGAYELAAYGYAGFWMQPTALDLSELQPRPTYA
                     RLAVYGASARGPLFGGLFNVEAAFYDSFDDWEGTDPTIPNSQFRGLVGYEHELFPKFQ
                     LGLQYYLEYTFKYDELIANSLWADYEQNEFRHLITVRLTQFLVMDKLELSLFVFFSPD
                     ELDTYIRPRITYKIIDPLAIVIGANLMFGRDDYSFFGQLEQNTNVYARLRYSF"
     gene            28791..28991
                     /locus_tag="VFG22_13525"
     CDS             28791..28991
                     /locus_tag="VFG22_13525"
                     /inference="COORDINATES: protein motif:HMM:NF022577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HET6417310.1"
                     /translation="MNIDKLVFRVAGIMVTTGVVLAYSHHIAWLALPLFVGLNMFQAS
                     FTGFCPMATIAKRFGMTEGKAF"
     gene            complement(29013..>31098)
                     /locus_tag="VFG22_13530"
     CDS             complement(29013..>31098)
                     /locus_tag="VFG22_13530"
                     /inference="COORDINATES: protein motif:HMM:NF021235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HET6417311.1"
                     /translation="TIVQAQCVTCHEADLKGALSPPHDSLPTTCQDCHTERQWSPAVS
                     TTIHTPLPASCVSCHQNDPRPETAFHIQVVDTDCVSCHRNYPDWTQANFLHTPAPRAC
                     LDCHSDRRPDTPTHQTPQGQGDCASCHIVSDWAVTDPAALHTPLPTSCVTCHENDQQP
                     QTIFHSQAAETECASCHTNYPDWTQTDFAHSPAPSTCNACHSDLRPTNPTHQSTAGRG
                     DCASCHVVDDWAATDPAAIHTPLPASCRTCHQNTPQPQTIFHTQAFDSECRSCHRSYP
                     DWKPADFLHVPAPGTCNACHSDLRPATPTHQSAAGLGDCARCHVADDWTVTDPAALHA
                     PLPASCRTCHQNEARQPTIFHAQIFEPECASCHRDYPDWTQVDFLHTPVPSSCLDCHS
                     DRRPNTATHQTAAGQGDCASCHLVTNWSAADPATLHTPLPASCLTCHQNEPRQPTIFH
                     TQVTQRECASCHRNYPDWTQTDFAHTPVPSTCNTCHSDLRPSTPTHQTTAGRGDCAGC
                     HVVNDWALADPVAIHTPLPAACIGCHGNEPRLNSPFHSQTYDPECATCHHSYPDWTQT
                     AFAHVPKPTRCYDCHEWQRPSTSTHPASGDCLGCHATSAWLPATGGAHPESAFPIQSG
                     AHSRYRNDCASCHNVALGSPVAGQNTDCVGCHAGEHTRAKMDPKHREVRNYPTGSAPP
                     NFCLDCHSDGRH"
BASE COUNT         5673 a         9485 c         9721 g         6219 t
ORIGIN      
        1 ctagggtacg tgagcgctgg cagatggtcg gcgcgcacat cgcgtcggct tttcgacttc
       61 gtcaggggtt gaagacgctg gcggcggact cccgccgcgc ccacacggat cttcctcgga
      121 atgcggaagc cgtactcgac ctggaagact ttcgaattgt cggtcaagcc ggccgcgccg
      181 agggcaaccg tgtcctcaag actcttcgcg gggcggcacg ctccgtagac caagcgcgag
      241 gcgcgcttcg aggtgacgac ccccagaaag cgctcgagat ctggaaggcg ctcatcggcg
      301 gccgctggac catggtggac tggttcgaca ccgacggccg ccgcttcgtt ctcgcgattc
      361 ccaactcacc ggagctgggc gagccgcgcg ggcttacaga gcaagagtca caggtcgtga
      421 actaccttct gctcggcgat acgagcaagg tcgtcgccta ccgtctcggt atttcctcgt
      481 cccgcgtttc gggcttgctc aagtcggcca tgcggaagct cggcgtctcg accaaggcac
      541 agatgatcga gaagctcagg ccattcgtcc cccaagaaac gcccgagggc gacggatcgg
      601 cagccgtcta atctccaagc gctgccctgc cgcgccgctc cgcatcgtcc atgccgcgag
      661 agagaagccc gggaaacagg cctaggaagc ggattagctt tccgcgcacg ctcggcacca
      721 gaacctcacg gcgtggctcg cgtatcgcct cgacgatcgc cctgccgatg gcttcgggcg
      781 ccatggcctt ttcggtgaaa acgacgacgc cagcccggcg cttgcgcatg ccttccagca
      841 ttggggtgtc cacggccccc gggcagacca gcgtccaatc caagggcgca tgacgtagat
      901 cgagagcgca gctgtgcgtg taggcgcgca ccgcgtgctt cgttgcgcag tacgcggcga
      961 ggtcgggggc cggtgcaaac gccgccatgc tagcgacgtt gatcacgtgt ccgcgctggc
     1021 gttcgaggaa ccgtggcagg atggtgcgca cgccgttcat cactcccaga aggttcactt
     1081 cgacggtgag tcgatcgtct tcggcagaaa cctcattgca ggggcctgga aggcacacgc
     1141 cggcgttgtt gacgagaatg tcgatggggc cgccttgccg ctcggcgctt tcgacgactc
     1201 tttcccaggc ggcgaggtcg cggacgtcca ggatcgcggt ctcgatccct ggaagctctt
     1261 cccggagtcg gacgagcgct tcccgatcga tgtctactgc gacgacctga tgaccggctt
     1321 cgatcgcgag cctaaccgtc gccgagccga tgcccttcgc cgccccggtg atcaggattc
     1381 gcttcatgcc catggtcccc atttgtacat cttcagcaga agtctgtctg cgacgccgtc
     1441 cggcagtgcg ccgaacatcc gtgctgccgt tcgtgcagat ctgggaatga agtatcgagt
     1501 cttgggtgat tccgccgtgg cggctttgac gaccgctttg ccgacttctt cgggcgcaac
     1561 agcgctcttg agctgctcgg tcgcgaaatc gtcgagatgg gacatgagct gccggtaagg
     1621 gttctccggg tcatctcgca agtgcgcgcc agcggccctc gcagtttcga aagtcggcgt
     1681 gtcgacgaag cccggcacga tgacgctcag cttgatgttc cacggcgcca actccagtcg
     1741 caggcaggcg ttcatggcct ccatgccgcc cttggtggcc gcataggacg cagcgagggg
     1801 cgcaccgaca tagcgagaca gcgagctgat gttgacgatt cgtccaccac cctgcttgcg
     1861 catcgtcggc gtgagctccg cgatgagttg catgcccgag agcaagttga cgtcgaacac
     1921 gtgacgcaga gcctcacggt tcatctgctc gagtgcgtag ggcgggccgt agccggcgtt
     1981 gttgacgagg acgtccactc ggccgaagcg ctctagtgtc ttcgtcagca gcgccgaggt
     2041 ttgctcggcg tcgctgaggt cggtcggaac gctgaacgcc tcgccaccgt cggcctgaat
     2101 ggccgaaacg agagcatcga gcagatccgc ccgacgagca gccacggcaa ccttgaaacc
     2161 tgccttggcc agacaatgcg ccgttgcttc accgataccc gacgaggccc cggtgacgat
     2221 ggccacgcgc tcgtgtttat ctttctcacc catttgtgct ccgagtctgg cgggctacgg
     2281 ttgcctgcgc cgcccgatcg cgctccggaa tctcggcgat cttcacgacg gagagctctg
     2341 cgttcatgtc ttcttcggcg agaagcgtgc gcagcacgat ctgtccacga cggtagccct
     2401 gggtgctcat ccagttgcgc acgccagggt tggtctcgct ggcgtagatc cggaacgttc
     2461 catcgtcgtc gagctcgact tgccgattgt tgatcgcaac ccggtgatgg cgatagtcac
     2521 ccgactcgag gaactttgtg agaatttgag cggaccagta gcggcagggc acgtccttgc
     2581 cctcgatgag gatcgcctcg tcgtcggcga gcttgaactg cgcgccgaca tacccgattc
     2641 cggggcccgg caggttgttc gcgaccacgc gcggatcgac catttcagcg agctcatcgg
     2701 ggctgtattc ctcgtggctc tcatgatcta gaaccaggtt gccgtccgat ccgatgcgcg
     2761 tcgggctggt gtcggtacgc tcgtactcga tcgtgttgag cccgacgaac gcagagattc
     2821 caagcgccga atccaaggtt tcattcatga aggccaccat gcggtcgagg cctcgggcga
     2881 gatgatcctc gtctagctcg ggaggggccg ccggatggtc gatgttttcg atctccatga
     2941 ccgcttggcg atactgcccg gcgtcgacct cggtcacgaa gtctgggaaa tactgacggg
     3001 tcagcacgaa cggccgtcgt ccctcgagct tcagccagtt cgtggcgcca tcgggcctct
     3061 tggcgctgag gtggatcttc gcaacgcgcg cgccgtcctg ctcgtcgaag acgatgcctt
     3121 cgtcgttgag atcatccacg atcagcttgt cgccgtcgtc gtcctccgcg tagacgacga
     3181 cggaaaagaa gaggtcgtcg ccgcgccgaa ccgtcatctc gtatgagcgc tgagggtcga
     3241 gcttcgcttc gtgatagagg gtgtcggtcc cctcggcgat gaacttccgc gtcggcgtga
     3301 acatgcgatt cagagacggc ctctgccggt cggcttcgag ctcgagctcc tgcgccgcgc
     3361 cgatcaagcg aatgaggtaa cgaaagcctt ctgcacgttc gcgggcactg cgagcaccgg
     3421 tcgggcccac taacttgtcg ccgagcttct tcagctcttc ggcaaagttg tcccaggctg
     3481 attggctgat cgatgggttt ttcgcggaca tgagcggcga tctgtcatgt gatgccgccg
     3541 ccgtaaagac cccgactagt cgagagcttg gaaatcgctc atgtggctca cgagctcgtt
     3601 acgcgtccgg aacccgagct tgcgcatcgc actccgcaag agggttgaaa cccgagaggt
     3661 cgagagaccc aggcgataag cgatcatctt gttgctgtgc ccgagaaccg cggcgccgac
     3721 gacttggttt tcgcgctcgg ttagaccacg gggatcggtg acgctcggct ggttcgggac
     3781 ggcgagcacg tagcgcctct cgtccgagtc gaaccaatcg accatggacc agcggccgcg
     3841 taccatggct ttccagatct ggagcgcctc atccgggtcg tcccttcgca gtttaccccg
     3901 ggcccggtcg acgcggatcg ccgcgtctcg aagcttccga gtggcgccgc tctgctgtgc
     3961 tgggccgacc gcgtccgtca gtcgaaagct gcgggcgtcg aagatggcct ctgcggcgag
     4021 cggaagctcc gtgccgcctt cttcggcggt cgccggggtg gagagagcac ggcgcaagcg
     4081 gtgcccggtt tcgacatgtg cggtcagcat ctgccagcgc tcggaatcct gacgcgaaag
     4141 cgccgtgatc tccgcgagtg gtgctacaat tgcgacgccc gcgccatgga gatccacggc
     4201 ggtcagaaac agcacatcct cgccgtagcc gacgtgagaa acgtagtgtt ctagatcttg
     4261 tggacgattc gtgaccttgg cgtactcaga gccggtcgtg gcagcgatcg ggcgcagctg
     4321 atcgcgaagc acatctggct cggtggtcgc cagggctcgc aagtgacgct ctgcgaagtc
     4381 ctctggaccc gaggcgacat gaaagcgcgt gatctcgacg tcaccctggt ctgggggccg
     4441 ggcgtactcg tagccagcga cgccgagtcc ctgatcgagc atgggcaagc cgcgtcgcaa
     4501 gagctcgggg agccactctt cgcgcgagac ggctaggtcg taagcggcct cggtgacgtc
     4561 gatgatcgcc gagcttgttg gatccattcg atgactctcc gtgcctcata aacggctacc
     4621 gggcgcggcc accatgccta cgcacccaca ccaactcacg agatccttca tttgaccccc
     4681 gatcccgtct ttggtccata cgcaattctt cacctattgc aggcgccaaa tataggaatt
     4741 ttccgcctct gcggctattc acggtactcc gggctctttg ggtgccgctt cggtataagg
     4801 gcttgtctgc cgcccaatcg aaccccgaga ccaaccgtga acgctcgagt accacgcatc
     4861 tgcatcatcg gagccggaat ctccggcatc gccgccgcca agtgcttgaa agagaaaggg
     4921 ctggcctacg attgcttcga ggcgtcttcc accctcggcg gcaactggct gttccagaac
     4981 ccgaacggca tgtcgtccgc gtatcgctca cttcatatcg acacgtccaa gcagaagctc
     5041 gagttccccg acttcccgat gccggacgag tatccgacgt tctgccatca cagtcacatc
     5101 caccaatact tcgaggacct ggtcgaccat ttcggactga gggatgcaat ccgctttggg
     5161 gctccggtgc gaaaggcgga gcgccgcgca gacggcgcct ggaagatcac gctcgatgct
     5221 ggcgtccggg actaccaaca cttgatcgtc gccacggggc accactggga cccgagctgg
     5281 cccgagtttc ccgggacctt cgatggtccg acgattcatt cgcaccaata catcgaccct
     5341 ttcgacccca tcgatttgaa aggaaagcgg gtgctggtcg ttgggattgg caacagcgcc
     5401 gtcgacatcg cctcggagct cagcaaccgc ggcctagccg agcgcctggt cttgtcgact
     5461 cggcggggcg catacgtcgt tcccaaatac cttttcggca aaccgatcga tcagattgtc
     5521 gagacgaatc cgtacattcc gctcgcagtt cagcgtaagg tcgcagcctt gttgattcga
     5581 tggctggtcg gccgcgtcga ggactacggc atgcccactc ccgaccacga gcttctcacc
     5641 gcgcatccca cggtctcgag cgatctcctt cagcgcgtgg ggaacggcga catcatcgtg
     5701 aaacccaacg tcgccgagct tctcggtgat cgagtctgct tcaccgatgg aacggtggag
     5761 gtgctcgacg cgatcatcta cgcgactggt tacaagatca cgtttccttt cttcgaccca
     5821 gagctcatcg cggcgcctga caacgtcttc ccgcttttta aacgcgtctt catcccgggg
     5881 ttcgacaacc tgatgttcgt tgggttcgct caagcgatcc cgagcatcat cggattcgtt
     5941 caagaccagg cgaaatggat cgcggcatat ctcgcaggcg agtacgcatt gccagccatc
     6001 gacgagatgg aagcggccat cgagcgcgac gaagctcgca tgatgtccca ctacgttgct
     6061 tcgagccgcc acaccatgca ggtcgaccac gtgctctatg cgcgggacct ggcgaaggag
     6121 tggaagcgtg ggcggcggcg cgcacgcgcc gggcttcaac acggttccgc tgttagcggg
     6181 tgatgcctcg cttcgggacc gtgaagtcgt acggcgctat ggggaggtgc tcgaagtcgc
     6241 tgtctccgag ctccggtcgg cacaggactc ctcggtcgcg cgccagcgcg accccggcaa
     6301 gtttcatcgc ggcatattga gtgtcgtggt agtcctcggc cacgacgagg tatgccccgc
     6361 tcgggctgag ctcgatccga cgaatctcga atggcaatcc ggacgtgagt gcggcgagct
     6421 tttcggtcat caccgcatcg gtcagctttt cgacgccgtc ggcgagccct tccttgacga
     6481 ggggcacgaa cttgttgttg caggagggtt tgaagcgcga gtagggctcc atctctaccc
     6541 gcacggagga atcatcgagg gtgaatctgg gcgcaccagc cactgtgccg agctcgccac
     6601 agaatcgaac gctcatcggg tgagtcttgc aatcgccgat tccgaagagc gccgtgaagc
     6661 ccgcgacgat gctctcggcg atgatattcg gtccgctcgg ccgcgtacga agcttggtgt
     6721 tcgtcgagaa gtagcttcgc aggcagactt ggcgcgcgcc ggtaccggtg acttgatagt
     6781 ggaactcgct cgggatctcg cgtggccaaa ggttggtgaa tgcatcgaag aaccaagcta
     6841 cgtcctgcat ctcgttcgtg ctcgcggacg cttcgcgcgc agagtcgagc tcgtccaatg
     6901 tcaactccgc aagaccccga tcgcccagca tgggtccgag catggggtcg agtcgagggt
     6961 cgcggtcctc ccacttccac ggaacgaaca ccgagcaagc gccgaggtcc aggttgtgcg
     7021 cactctgcag gctccgctcg agtggctgcc ccgtccatgg ggtctcactc cactcggcgc
     7081 cgacaccatc ctcggcgggt tgccaaggag cggtggtctg cgtccaggag tgacgcccgc
     7141 ttatgaggcg cgagaagtag tctgcctccc aaacgacatc actcgcatca ccgatgtagc
     7201 tggtcctggg gcgatcgagt agctcaccgc cggctcggcg ttcaccgcag tgcactcggc
     7261 cgacggtctg tacgtagtct gccgtgcaga catgcatgtc ttggcctatg cgcctgctgt
     7321 gtgggatcac tgcgccattg tcggtgatga cctgcaggcg ggttgcgcag ccgatgccct
     7381 tgcattccca cacggaaaac ggaagtcgct cgaagaagac gaaccgcgcc ttgtcgactt
     7441 ggttcgcgac ctcccaggga aacccagcaa ggagaaagtt cgacttcgat cccgttccag
     7501 ccacaatggg cgcttcgaga atgttctcga cgacttgata cccttcgctc ggatacgaga
     7561 tctcgtagca gccgcagaga agcgtcaata ggagtgcgag ccaatgtcgg atgaggcctc
     7621 cagacgctcg actctacggc gagagggcgc gaggaatcaa ctgatcgaca atcgccaggg
     7681 agcgggcgac gggattcgaa cccgcgacaa cctgcttggg aagcaggaac tctaccactg
     7741 agttacaccc gcaaagagac gcaagaacta gccccgcttg ccttggccgt caagcaaggg
     7801 cccccgcgaa tgggagcccc cgtctgacgc acgatcgtcc gcaatgggtg attcgcgaag
     7861 cgctcgcacc gttgctctcc accgtgctcc tctacgaccg ctcggatgcg accttgctcg
     7921 actttcacca ccgcgtgagc gtcgaacgcg acgttgatgg agcggttctg aaatgcagtg
     7981 gcgccgttgt tgtcggggcc gcgcacgagt acctccctca agccgcgttt ttccggtaac
     8041 ttgcgggcag cactgcggaa tccggtcgcc gatcggagtt tcctgggctg tgaggtagca
     8101 agctgaggtc cagggcgttt tggaggcgta gcgtgtcccg atgagagaac tgttgcgagc
     8161 gacggctcgc tttgggcttt gcctcggctt gctgatggga ctcagcgccg atccggcgga
     8221 ggcgcaggat ctcgtcgcgc cgcgggtgaa gtcgcttccg gggatcgagg taccggaggg
     8281 tgtcgagatc ccgaagtcgg gcgtcgttca ggcactcgtt cggatcgaca tcgacgggaa
     8341 ggggcaggtc caaaagtgca acgcaggtag atcgctttgc gatctagtga tcgaagggat
     8401 taagcaagcg gagttcgagc cggcgatgcg cgatggacat ccagttcctt cgcaggtgag
     8461 ggtggacctg cgtcttagga aagaacagac gccccagtcg acccgcgacg aagcgctgct
     8521 cgaaccttcg acgcaaaccg tcgaaaagga gctgattttt tccgaaaccg ccgaggtcga
     8581 tgcccgcgtt cagaccccga tccggttcga gctcgatgga attcggaaca ttcccgggct
     8641 cttcggcgag ccgtttcgaa tctgcgaggt tctgcccggc atgctgcccg tcgcaaacgg
     8701 tcagccctat tgctacgtcc gcggatcccc accctcgggc accctgtacg tctacgacag
     8761 cgttcccatt cccttgctat tccactccgc gctaggacct gcgactgtgc ttccgggcct
     8821 tatcggcacg ctcgatactt actcaggcgc agcgccggcg cgctacggag ggttcacggg
     8881 tggggtgatg gcggccacgg cgcgcgagct tccgaccgat cgcgttcacg gagaagcgga
     8941 gctccgcctc atcgatgtgt ccgcgctatt gaacgtgccg atgccaaaag agggttcgat
     9001 gacttttggc ggtcgctacg gattccccaa cctcttgcta ggtgccgtca atgtcgacgc
     9061 cgacgtgaac tactgggact accagtatcg tacgggcgtg gccatgagct cgaagtctcg
     9121 gttcgaagtc tccgcgtttg gcgctcgaga tgcctccgtg ttcgacgatg cggatcccgc
     9181 cgcacgactg gccctcgatc ttcagttcca ccgggtcgac ttgcgtttcc ttggcaaggt
     9241 caatcgatgg gacctcgtgg gcacgatgct ttacgggtac gatttctcga acgtcgacga
     9301 cgggtctgga agcgcggtct ccgacgccgg tgcgagcatc caccgtttcg ggccgcggtt
     9361 ctgggcgacg tacggcgcgc ccaagtttca agttcgaatc ggtggcgacg tggttggtct
     9421 gtttggaccg gccagatgca cgcccggcat ccagaacgtg tccactccct gtgaccccga
     9481 attcgctgcc caggacaggc gtgtgatcgg aggcgttttc gtcgatggca acatttcgcc
     9541 gatctcttgg gtagaccttt cactcggact tcgagccgac gtctggggta ccgcgggcta
     9601 ccgtgacgcc gcggccagcc cgcgggctcg agtgactttt caccctcacg agttggtcga
     9661 cgtcttcgcc gggtggggat tcggatttag gcccgccacc tttgcgattc cgcttcccgg
     9721 gcttggtgac gtgccactcg aacccggact tcagcgcgca aaccagacag tcggaggaat
     9781 tcggctcaac ctgccgcacg agctcagctt cgaagcgcgc ggatatctca atctctttag
     9841 ggaccttcgg ttcgtcgacg tgttcatgaa tcccgagatc tccgacgatc cggcttcggg
     9901 ccctcttccc gccggggcac tcgatgattc agccgacgga agaagctacg gtctcgagtt
     9961 gctgtttcgg cgacccttcg ggatcggatt ctccaccttg gcgagctaca cgctcgccta
    10021 cagcgacctc accgcgaccg cgcttctctt cgacgcccca cctcagacct tcgactacac
    10081 gccttcgtac gacgtgcggc acgtcatgaa tggagcgctg gcttggcaag cggactttgg
    10141 cctcatcatc tcggcccggg tatttgcgcg ctccggtcgc gccgagggtt ggctctggcg
    10201 ggacggttcg ggccaggtgc agcagtacgt gcagcgggtg ccttggttcg tgaggctcga
    10261 cgcggaaatc gcttatcaat gggcgaaacc gggtcgacgt atgcgaattt cgctcgagtg
    10321 gatcaacatc acccaggcac gggattccca agaggtcgat accagcgacc cctctgcgcc
    10381 attcgagtgt cggattcgct ggggcgtacc ttcggggccg tgtccgatca agttcacttc
    10441 ggcgatctgg tacccgaacc tttcgttccg ggccgtcttc tgatcgagcc ctagctctcg
    10501 gcggcgactc cgtcgactgg tggggcttta ccagtcgctt cgcgcccgat gccagaccag
    10561 tgatggggat tgagagcgcc ggccgcgggc ttcacatcta ccaggaaccc ggtgagatgc
    10621 gccatgccag agactttttc gagcctgtcc gggccatcgg cggccaggag gttcacgttg
    10681 acgccggtgg tcttgctggc cacccccatc ttgctgtcct ggtgtcccca gccatggggc
    10741 aaggccaccg tgccgggcat caactccgaa aggagcttga ccgggatacg cacggtggcc
    10801 gtggccgtag aaacgtcggc gagactgcct tcgccgaccc ctgcgcgcgc cgcgtcgtcc
    10861 gggtgcatgt agagatggtt ggtggtgcgt tcgccagaaa cgaactcttc gatgttgtgg
    10921 gtccaggagt tgtgggtggt aatggcccgc ttggtgatga gcttcagcct gtccttggtt
    10981 cgcagctcga gctcgaaatc ggattcgagc ttctttgctt gctcgaggaa gggcgagggc
    11041 gccagatgga cctttcggtc gtcatggacg acccgcttgc cgaggaagct tccgggttcg
    11101 tgctgcgggc gcagacggcc atgtttctcc ttcaacagtt tcttgaagct gccttgcttg
    11161 gtcgctcgga gcagccccga gaggagaagc tcctgcggta tcgaaggctg ctctccacga
    11221 cgcccgaccg agttgatggc cttggcggtt tcaaacgtgc gttgggcgag cgccgacccc
    11281 cagagattga ccccgctcgc actggcgatg tcgacgtaga tcgtcgcctc atcgcgctgc
    11341 tcgccttgcg gaggaaccac ggcgcgggtc gcttgcagat agggcttcgc ctgaagcccc
    11401 agcatcaacg gaaatacgaa cggaaggtcc ggacgctgaa acggcgacgt gcaaggaagc
    11461 acgtgggtgg ccacggacgc agtctcgctc ggctggatgt ccaggacgac gagcagctcg
    11521 agctctttga atgcctcgcg cagacgctcc gagttcgcca tcgtgatgag cggattacca
    11581 ccggtcacga acaaggcctt tatctgtcga tcccccggtg tgaggatctc gtctgcgagg
    11641 atgccgcccg gcatcgaatc attcaccgac tggaacttgc cgattcggct ttcgttttcg
    11701 tgaagaagcg tgcccgtctt ctttccaaat gccgcgaagt cgatgacgcc cgtgctcaca
    11761 agggtgcccc cttcgcgatc gagattgccg gagatcgcgt tgatgcattc ctgcagccaa
    11821 aacgcgagag acccgttcgt gcccatgttg acgcccgtcg agcagtagag cgcagcgccg
    11881 tcggcctcgt tgtaggcgcg caccatctcg cggagagttt cggccggaat gcgcgtgacc
    11941 tcagcggtgc gttccgccgg ccaagcctct gcgatgcggc aaacctcgtc gaatcccgtc
    12001 atgtatcggt cgatccgcgc ccggtcgatc tttgccgtcg acaagagctc gttcaggaaa
    12061 gaaagataga agaagacgtc ggtgttgggc cgaatgaaga cgtgctcgcc ggcaactttg
    12121 gcggtctcgg tgcgacgcgg atctacgatg aagatctttg cgccgcgcgc ttcaatctcc
    12181 ttgagacgcc gaatcggatt tgaaacttgc agaaagctcc acttcgagat cgctggattg
    12241 gcaccgacga tgatcaggca ctccagcttg tctaggtctg ggaagggctg cgtaaacgga
    12301 aagccgtaga cctgccgcgc aactgcaaac ttgttggcgc agtcctgcgt cgcggacgaa
    12361 aacatgttcg ccgatcccac accttgcatg aagccttgcg cgaacaccgg atgcaggact
    12421 ccgaatccgg cggccgtgcc cacgtacatg gcgatgctgt cgcggccgtg ctcgtcgatc
    12481 aactgccgaa ccttaccgcc gatgctgccg agcgcgtcgt cccagctcac tcgccgaagc
    12541 tcgccgtcga tgcgctgcat ggggtagagc agtcgatcgg gtgtgtcgta catccgatgc
    12601 tgcttcaggc ccttgagaca tccgtacccg cgtgtcgcca catgttcggc gtcgggtttg
    12661 atgtcgacga cgcggttgcc ttcaaccgtg acctcgagcc cacagagggc ttcgcaaatc
    12721 cggcaaaacg tttgaactgt ctcggtcatg atcgaccgag tctatagggc ttagtcctcg
    12781 tggatcaact cgtcgaaagc ccgattcacg tcgtccatcg attccagcat gagatcctgg
    12841 tcgctgatgc ccgaagggcc gccgagccgc tgcaagcgtg ctcccacttc gcggaactga
    12901 ggttccctct ggaaaacgta gtcgtagtgc aggcgggcct gatcgctgag gccgctctgc
    12961 tcgcaagcca tgccgagctc gtagtgcagg cccacggctt cgaaaggggt cttctccggg
    13021 ctctcgaggc cggattggaa ggcttccatc gcgtcgcgcg gctgcccctc ttccaagtag
    13081 attagaccga tcatcgtcca gcacagacag cgccgtcgtg gatcactcat ggcgacttgg
    13141 aattcacgtc gcgcatcggg gagcaagccc atttccttgt acgcgatgcc gaggtcgaag
    13201 tgggtatcgc tgtcgtcgtc gctcacttgc gccgcgactc ccgccttgaa ctgctcgaag
    13261 acggtctcga cgtcgatcat ttcggcgcct tcgtccatgg gcgcgatctc ggcgacctcc
    13321 gcaagctcct ccgcgagctt ctcggcaaaa tcgaatccct cgtcaccgta ttggagctct
    13381 tcgtactcga gctccatgtc cggtggatcg gaagcgagtg tagccggcat ggacgaaacg
    13441 gatggacgga tgagcggctg agcgaccgca gagacgcccg acgatgcagc ggcgagctct
    13501 tcaatctccc gcgccttgtc gagaacgagt gggtggtctg gaagggtcag ctgcagctcg
    13561 ctcacgattg cgcgcgcctg gttgaacagc ccgtcaacca agtacgagtc cgcgagctcc
    13621 aatgtttgct ccatcagact tggtgcaatc gctgcagttg ctgcggcgac ttgggtgctg
    13681 accagtggga gcgaaacaaa ctcgctgtag gacatcggac cgagtgtgcg catcggaatg
    13741 acccgaccgc ccgacggagg tctcggggca gggggcaccg aggcgtacga gtcttcgact
    13801 tcgatcaatt cctcggaaat tgctggtagc gaaccggaca gggtgctggc ttcttcgact
    13861 tcgacgtcct cttcgacgta catgatgccc gaggcgccgc tcaggccgga ggcgctacga
    13921 accgagggcg agctcaatga tgttgccgca ctcagcggtg ccgcggaaac cgaggtggac
    13981 ccagtcgcct ccggctgatg catcgcgccg gagggcatgt agtgctgccc tgaggcggaa
    14041 gcggttttct gaagcgccat tcgcgcttcc tgatcgttcg ggctcagctc gaggatgcgg
    14101 tgcaaggcgc tcatgcgttc ttgcggccga tgtgcttcac ggtggatgcg ggcaatctcg
    14161 cgatacacgc tgaccgcctt ctcggtttgc gagagcatca ggaacgcttc agcgagaagc
    14221 gacaaggtcg agacgtccct agggtcagcc ttgaaacaga tctgcaactt ggcgagtgca
    14281 cgcttcggat cgcgccgggc gatgtacgtc tcggcgagct ccttagcgac gtgaacgtcg
    14341 ttgcttcggt ggtacaggag gcgctcggcg accttgatgt aatcgtcgag gcggccttga
    14401 tcacgcaaca gcacggcgcc ttgttggaat tcatccgcgg cttcttgcgt gcggcccgcc
    14461 cgtgagagcg cctctgcgta cttgattcgg accggaatgt gctcggggtc gaggtcgacc
    14521 attttgccga gcatcgccag cgcctgatcg gtgtcgccag cgcgcatgaa gccggtcgag
    14581 acgtcttcga acacgctcat tgcgtcgctg atcaattgaa gctgctcgta catttctccg
    14641 agcttcacct gcgcatcgag gcgcgaaggg tcgagcttca ggatctgctt gtagacggcg
    14701 atcgccttga ggaagaaccc ctgtgtggag taatgccctg caacggtctc gtacgtttcg
    14761 atcgccgcgc cacggttgcc cttgcgcacg tgcaggtcgc cgatcttcag cagggaacga
    14821 acgtcccgct tgtcggaatc gaccagcttc ttgtactgcg cgattgcctt gtcgtattgg
    14881 ccacgcgact ggtacttccg cgccgcgtcc aataccttgt tcctgttgat agccaaagcc
    14941 cctgctaagc ccccatgcgt tcagtgcgcc tacacaagtc tactaatgac gcacggggga
    15001 ggtcaacatg tgctcgggca agagctcctc gagaaatcga gtcgtgtatc gcccttcctc
    15061 gaacgccggg tgggccagaa tccgctgatg aaggggaatg ttggtgcgaa tgcccccaat
    15121 gatcgtctcg ctgagagccc tccgcatctt ggcgatcgcc tgagcacgtg tcggtgcatg
    15181 tacgatgagc tttgcgacca gagggtcata gtgccttgga accaggcccc cgccatagac
    15241 gccgctatcg actcggacgc cggtaccgcc cgccatgtgg agctcggtga tccggcccgg
    15301 tgagggcgca taggtcacgg gatcttcggc attgatgcgg cactcgatcg cgtggccccg
    15361 cggcgtcagc ggatgggcgg gcagctcgag gacctggcca tcggcgatgc ggaactggtc
    15421 gtcgatgagg tcgacgccca tgaccagctc ggtgaccgga tgctcgactt ggatgcgtgg
    15481 gttcatctcg agaaaagtga gctcaccgcg ttcgtcgagc aggaattcga gcgtgcccgc
    15541 cgagaggtac ccgacctggc ccattgcgcg gcgaatggtg gccgtcatgt cgctacgttg
    15601 ctcgtcgctg agagccacgg acggcgcctc ttcgaggacc ttctggtgtc gccgttgaat
    15661 cgaacattcg cgctcgccga gcacatggat gttcccgtgc gcatctccga ggatttggaa
    15721 ctcgatgtga cgcgggcgtt cgaggtactt ctcgaagtag agtgcgtcgt tgccgaatgc
    15781 ggctctcgac tcggaacggg caatttcgaa gacggcccgc acctcttctc tcgaatgcag
    15841 tacgtgcatc gcgcggccgc caccaccgcc cgccgccttc aagagaaccg ggaagccgag
    15901 cccctcggct tttcgtacgg catcctcgac gtcctcgacc acctcggttc cttcgagtgt
    15961 gggaagcccg agggacttcg cgagcttgcg ggcgctgacc ttgtcacccc actgtcgcat
    16021 ttgctcagga agggggccga tgaaatgcag gccgcactga gcacaggttt cagcgaactc
    16081 ggggttctcc gagaggaaac catacccagg atgcacggct tcggcgccgg tgatctccgc
    16141 tgcagcgatg atcgcaggga tgtggagata gctgcgcttg gggtccgctg ggccgataca
    16201 gactgcctcg tcggctagcc tcgtgtgcaa agcatcggcg tcgacatcgc tgtggacggc
    16261 aaccgtgcgt agtccgcgtt cccggcaaga gcgaatcacg cgcagcgcga tttcccctcg
    16321 gtttgcgatg aggactttct taaacaccgt tcagctcggt tcgattcgaa agagccggtc
    16381 gccgtattcc accggtttgc cgtcctcgac gagcacctcg acgatggtgc atgcgagctc
    16441 ggcttcgatt tcgttcatga gcttcatcgc ttcgacgatg cagagcactt gacctgggac
    16501 gacggtgtca ccgacctcga caaagcagtc cgcttcgggg gaaggagccc gatagaaggt
    16561 gccgacgaac ggagaggtca cgaagtcacc ctctgactcg gcaggggcgg gctcggatcc
    16621 tgggggcagg ttgcttgctg cccccgcagg cgcgaccgaa tgcgaaatcg cgccgactaa
    16681 tggcgcaggg tcgcgacgca cgcggatgcg gtcgtcgtcg tgctcgactt cgatctcggt
    16741 gagattggat tcatcgagga ttcgaatgag gtcccgcagt tgatccagat ccaacttcat
    16801 tgtgtttccc ggccgcagtg ccctagccgg cagcgttgag atagtcgatt agtgcacgaa
    16861 ggccaagcgt gtagctgctc tttccgaaac caatcacgga accgatacac acggcggcag
    16921 tgacagactg atgacgaaac gattctcggg cctgcgtatt ggaaatgtga acctcgacga
    16981 ccggcaatcc ggtggcagcg attccgtcgc gcaaggagac cgaggtgtgt gtgagaccac
    17041 ccgggttgat gacgacacca gcgaaggcgt cccgggcatc gtgaagccag tcgatgagtt
    17101 gtccttcttg attggactgg cggcattcca cgtgcgcgcc gagatctttg ccgaggtcca
    17161 tgacggcctt ctcgatgtct ttcagggtcg tggagccata gacgtcgggc tcgcgagtac
    17221 ccaacaaatt gagattgggc ccgttgagca cgagaatgcg tttggcctgg gcggtcatga
    17281 gctcagaggt tttcgatgag cttgggcgcg ctggtacgga gcagataccg cgccttcccg
    17341 atgtttccgc ccggcgagat cgcgagggct acgaagtact cgtccgtcag catccggacc
    17401 acggtggtca atcgttcggt cgttaccgaa acttcattgg tagagcccgt gtcgagcatc
    17461 tcggcggcgc gctgcacgcc cttgagcacg acgctgtact ccattccgac cgcctcgatg
    17521 tcgaagggac cgtcacccgt cgaatattga tcgattgcga ttccgtccga ggccatcagg
    17581 agcccagcta cgccgccatc cgctcgctcg acgaggtctt gcagtatctc tttgaacatc
    17641 gctcctccgc ctagcaatca ccgcatggac ggcgcctcgg gtcaagtatc gcgtcttgcg
    17701 atgcgctcgg ctgttcgcac tccgccgagt gccacggcca aggtgctttg gccgagcagc
    17761 accgagtcac ctacgagata aaggccgcgc gcagctcgtc gcggccagag ggagcggggc
    17821 cggtagtgcc tgaggcctac gcgtctgggg actccgccta cgaacccctc gtgtcggccg
    17881 gtgaatcgtt cgaacgtgcg ggggcttgcg gtcagctcga agcgcgctcc ttcgagcagt
    17941 cccggggcgc ggcggcgaat cgtcgttctc attcgctcct gaatggctcc gacgtactct
    18001 gcccgtgcct cggcgcccag cgtgcggagg gccttcatcg gcacgtgggt cgatgccgtt
    18061 accgtgcgct ccccgtttgg tgtacgcgca gtctcgtcag cggcgctgat cgaacagaag
    18121 acgtggtttc cttccacgaa aggcgaggtt ctgtcgtcga cgagttgcag gtgatgagcc
    18181 tcgtctcctc caaccgcctc ggcatccact gcgagataga gcatgcatgc cccccatccg
    18241 tcttcgacgg agcgctccac gtcgtctagc gacccctcga acggcgtctc gctcatcgcg
    18301 gacaatgact gtggcaagac attggccacc accgctgacg aacggagctc gcccttccgc
    18361 gttgtgactc gccatcggcc gttctcgcga acgatgctct tgactcgatt cgccagctcg
    18421 acctgtccac cggctttctg gatcgcgtga accaatcccc aggcaagtga accgatgcct
    18481 ccatggacgt gtccagtgcc gcgaaagaag tagtctgccg ctgccatcgc aaacggcgct
    18541 tcggcttcct gagcgctcgc ctgcacggta atctgcgaga cagcgtccag atagatacgg
    18601 agcggctccc agtcggcgag cccgtgatga gcgacgacgt cactcaggga gcggcgcatc
    18661 caccggacga ccggcgcgta ggaatgcgtg cggtgcaagt gtcggagtag ggcgctgccg
    18721 ttcagagggg gcagcagact cggatcgtcg aagagacgcc aaagagcgtc agctaccccg
    18781 cgctgtgtat cgaagaaccg ctcgactgcg acggggttgg cgcctgggag cgcaacgaag
    18841 cgcctgagaa actccgtcgg atcggagggg atcttcagag agaagtcgtt ggttcgaagc
    18901 tcgaccagag ggtcgagtgc gtcgaatcga acccccaggt tccagatgcg attccattcc
    18961 gagaacaact ggccgtctcc gattcccgaa aagagcgtcg ctccagcttc aaactgatat
    19021 ccattccgcg tgaaggtgct ggcgcagcca cctggatatc gaagcgtctc gcagagcacg
    19081 acgtccgccc ctagctccgc cagccgcagc gccgtggcga ggccgccaaa tccagccccg
    19141 atgacaacta cgtcggcttc gcgcatcgag cgcttctaag gtcgccgcgc ctcctgtcca
    19201 gtgtgcctga gctcgaccgt gagacctccg ccgtcggtat cccgaagcgt gaggcccaag
    19261 ccatgccgcc gtgcaacgtc acttgcgatg tgaaggccca agcccatccc atgcggatga
    19321 cgagtgcgcg cctggttccc gcgaaatgcg cgttcgagcg cacgtttgcg ctctccttca
    19381 gggatgcctg ggccatcgtc cacgacggtc aagtcccatg catcgtcgct cccacgcaag
    19441 acgaccgcta cgtggccatt tgattcgttg tagcggagcg cattttggat gatgttgccg
    19501 agcgcctgtt cgagaagcgt gaggtcacct cggaagtgaa tagaggtctc cggtacggcg
    19561 gagtccagcg cgatgtgttt tcgtattgcg atcggtcggt gccgctcgac cacgcgctcg
    19621 accagtcggt tcagatcgac tgaatctcga tgcgccagtc cttcgatgga ttcgaggcgc
    19681 gccatcgcat tgaggttgtg caagagagaa cccaaatact gcgcttcctc gatgcttggt
    19741 acgactgcag ccacgtcgag ggtttcaccc gcctcgatcc gctgctggag cgaaacgaga
    19801 tggccttgaa gcacactcag ggggagcatc acgtcgtgcg tggtgttggc gatgtagttg
    19861 cgaagcgccg tttcgcggtc gcgaagggat tcgagctgcg cgcggatggt cagacggctc
    19921 tgctcgaacg cggcgccgag ctcggcgatc tcgtcgttgc cgcggacgtg aatgcgttca
    19981 ttcgagtcgt ttccgagcct tcgaaccgac tcggtcaagt cacgcaccct tccgacgatc
    20041 ggacctgcgg caaagagtgc gaccaagatc atgaccacgg cgaccagcag cgcgggcatg
    20101 agaaccgcgc cggagaacca tttgggtggg cccttggccc gcatcagcac gaccgagcaa
    20161 ggcccatcga cccagggcat gcgaaccgcg acctcggtgc gacgccggtc cggtgagcgg
    20221 ctcgcagcga tatcgtcgcc ggcttcgagt cggctcgcca actccggcgg aaaaggtggg
    20281 ttctcgggat tgaggctctg gaagtcggcc ccgtatgcga agatccgata tggcgggagc
    20341 cgacgcgggg gcgggccggg gcgccgctcg gaacgtcgca ccgactgcgg tccaggccaa
    20401 cgtgcagggt cggcctcgca acgttcgcgc tcgccggatt ccatgcgatg caccgcggcc
    20461 tcggccaaag cctctccgcg cagttgcgaa tgccagcggg attgggccca acccaggagt
    20521 acgagcaagg aaaccgtcgc caggctgagc gtcaacgcca atcgtgcccg cagcttcatg
    20581 cgtcttccga gagtcggtag ccgatgcccc agaccgtttc gagtcgtgtc gcgtgctctc
    20641 cgagcttgct gcggagcctc gagatgtgga cgtccagtgt gcgtcggctt ccctcatgtg
    20701 cagggtcgag ggcagcgtcg actagcgcgt cgcgcgtcac ggccgagcct ttccgccgag
    20761 ccaaaagaga gatgatctcg aactctaccg gtgtgagtga aagcactccc tggccgtgac
    20821 tgacttcgcg acgctcgagg tcgacgcgga tggggccaac ggtgatgagc ttcgtgctca
    20881 ccagattggg gcggcgcaag caggccttga tgcgtgcgat caactcttcg ggaaagaatg
    20941 gcttcgtcac gtagtcgtcc gctccgagct cgaggccgcg gatcttgtcg gccgtgtcgt
    21001 ctcgagcgct caagatcagc accggcacct ccgatttcac acggaagcgc ttgagtagat
    21061 ccatcccgta agtgccgggc agcatgaggt cgaggacgat cacgcgatac gcgtcgggat
    21121 cgagttggcg agcggcgtca ccatcttgga cccagtgcgg atcgaacccc gctcgtgaaa
    21181 gcgtctcgac gacttgctgg ccaagggcga ggtcgtcttc cactagcagg attcgattcg
    21241 tcaccgagcg ctccagttac ttggtttctt cggcgtcgcc gcgcccgaag tgccgttcca
    21301 tgcgttccac tttgtgctcc cgctggagca gtcggagatc atcgcgctgc tcacaactga
    21361 ggttggcata gatctgatcc tccgtcgcga aacggagatc cttcatggcg acgaatttct
    21421 ctttgcctcg gggcatctcc ttgatttcct caccccgcgc cgccgcgtcc tccatgatcg
    21481 cgcggaccgc ggtttcctgc gcggcgctca ggttcagccg ttcggacatt tccgcaagat
    21541 gctcctccat tctttcgggc gaccatgggc ctccctggcc gtgggggcct ccgtgcggag
    21601 caccgtgcgc gagcgctacg ccgcctacgg tcagagcgag ggccgtgacg ccgccgagaa
    21661 tggcggcgac gaatgtttct ttcttgatga gcttcatgat tttcctttcg ggaggactcg
    21721 cctcctcatg aagtaaacgg ctcgaatctt aagcgggtct taagacgggc gatcttttct
    21781 gttttccgct agtttcagcc cgtggctcga tccttcttgg tgctcgatat cgaaaccgtt
    21841 cccgacgccg acttggcttg ggatgccgcg gtcgatgggt ttccacccgc tccgtttcat
    21901 caagtggtgg cgctcggggt cctgtggctc gacggggacc attctctcaa aaagctcggt
    21961 gcgtttggcg gtgaggaaaa tgcaccgccc gacgaatcga aagtgctcgg cgagtttgcg
    22021 gagttcatcg gagagcgcca tccgaccatg gtgaccttca acggacgacg ctttgattta
    22081 ccagtgcttg cgaatcgcat gctgaagcac ggagttccgt ttcccgcata ctacgccggc
    22141 cgcagcgaag gccccgacta tcgctatcga tacaacgacg agggccacat ggacctcgcc
    22201 gatctgctca ccgactttgg cgcgagccgc atgccgagcc tgacggccct ggcccagttg
    22261 gtgggcatgc ccggcaagat ggacgtggac ggctccaaag ttcaaagcat gtacgagcac
    22321 ggccggcacg cagagattcg ggattactgc cttcacgatg tggtccaaac gaccttcgta
    22381 ttcctgcgct ccgagctcct ccggggccgc ctttcgaagg acgagtatcg caaccgagcc
    22441 gaagccctct ggaccgcgct agagagcgat cctcgcgccc tcccggttct cgagcgagcg
    22501 aaccaagcgc aggcgcttct cagtctttga gtggcgtcag acctcatttt cagggcgcct
    22561 taaggcatct gtaaggtttg accgcgtcgg tctgggtgaa tgcggggctg ccccgcaacg
    22621 actcatgaaa gacgagggac gaacatgcga catctacttc attttgggct ttttgtgttt
    22681 tttctcaccg ctgcgacgcg ggccgacgct caggataggc gtcgggtcta tccgcagacg
    22741 agggtcacgg tcgtttcaac ggatggcctg cgagccgctc atcccgggcg cgcgtacttc
    22801 gaggagcgcc aagacctcga agagatcacc cggatcgcgc gaggttggta ccgggcagcc
    22861 gcgaacggca accggcgcgc cgagctcgcg ctggatcgtc ggctcgacgc ctggctcgat
    22921 cgggagatcc gtgaatcgat ccggactccc cgtgatcatc gatacgcgat gcagatccgt
    22981 gcgctcggcc agcagctcgc aacgctcgag cgacgccctt atcacgccta cggccacggc
    23041 cacggccatg gccatagcca cggccaggtc tcgcatcgag agacccatcg aagcctaggt
    23101 ggctactatg agcggaaagc catgatcatc gacgagctag tcgagctttc cgagcaacgc
    23161 gtgagaaatg cgtacgcggc gattcaccga ccgattcgca tgtcgtttgc ccaccgctga
    23221 gccacggagt cgcctcgctc gaaagcgcct cggatcaaac cggggcgctt tcctttcggc
    23281 tacccactga acgcgcgccg ctcctcgtcg agtgcgcgct cgatgttgcc gcagacgatt
    23341 gcacagagct cgaagaccgc agggtcggcg acagagtagt acgcggagtt tccacgctgc
    23401 tcgcgcctga ccagacgatg cgcaaggagg gtcgtgaggt gcttcgacac gttgggctgg
    23461 gtgctcccgg tcgcctccac gagagcggtc actgtacgct cgccgtgggc cagctcgttc
    23521 agaatctgca gccgaagggg cacccccagg actcggaatc gcgtagcgac agcatccaga
    23581 ttctgcggag tgaggacggt tttcggcatg gcccctccta tataaggcca atctcgagaa
    23641 aaatcaccac ttgactatat gcttatgtgg tcatataggt gtgggcaagt ccaaacggag
    23701 ttgaccgcat gaaacgaaag attctcgatt ttccccttca gcacgctcgc ctggtcttcg
    23761 tcggcctggc ggttctcacg atcggcctcg gttcggccat cccttggatc caggtcgaca
    23821 ccgacccgga gaacatgctg tccgagcggg atcccgcccg cgtcttccac aaccagacca
    23881 aggaagagtt cgcgcttcac gacctgatcg tcgtcggcgt ggtgaacgag gtcgagcccg
    23941 ctggggtgtt caatcccgag tcgctcgccc gcgtccacga gctcaatgag cggctgaagt
    24001 cgatcgacgg agtgatcgcg cgcgacgtgc tcggtccctc gaccatggac aacatcgagc
    24061 aggcgggtcc cggggcgatt cgattcgagt ggctgatgga acagccgccc aagacacccg
    24121 aggaagcgct tgcgatcaag gaggcggcgc ttcgattgcc gatgctgcgc gggacggtcg
    24181 tgtcggagga cggcaagagc attgcattgt atgtcccgat tgaaagcaag gatcagagcc
    24241 accgaatctc ggaagagatt cgaaccacga ttgccggatt cggcgacaac acggacgact
    24301 tctacatcac cgggctaccg gttgcagaag ataccttcgg cgtagagatg ttcaagcaaa
    24361 tggcggtctc cgcaccgttg gctgcgctcg tcattcttct cctgatgtgg tggttctttc
    24421 gatcctttgc gctggttctc tcaccgatga tcgtcgcgct cacgacggtc acgatcacga
    24481 tgggggcgct gatcgcaagc gggttcacgg tgcacatcat gagctcgatg atcccgatct
    24541 ttctcatgcc gatagcggtc gtcgattcgg ttcacttgct ctccgagttc gtcgatagct
    24601 atgcgaagca gcgcgatcgg aaagcggcca tcaaggaggt cgtcggccat ctgttcgctc
    24661 cgatgctgta cacctcgttg acctcagctg ccggctttct ctctctgacg ttcactccaa
    24721 tccctccagt tcgcgttttc ggagtgtttg tcgctctcgg gattggcatc gcattcgtcc
    24781 tgaccattct cttcgttccc gcatacatcg ctgtgcttcc ggagaagcgc ttggcagcac
    24841 tgctctcgag taagcgcgac aaaaaagacg gcggacttct cggtcgcgcg gtcgctgccg
    24901 tgggcgacaa gagccttcga ttcgccaagc cgatcctggt cgtgaccgtc gctgcaatcg
    24961 cgatcagcgc gtacggcatc accaagatca acatcaacga caacccagtg cgttggttca
    25021 aaccttctca tgagattcgc gtggccgacg ccgtgctcaa tcgcgagctc gcggggacgt
    25081 acacggcgta tctcatcatg aagcaggaca ccgatgccga gcgccagatc gtcgaagcag
    25141 gcgacgccgc ggtccaaggc gctagcgatg gggtgcgtgc caagtgggcg gagctcaaga
    25201 ccgaatcaga gactgccagc agagagggcg tgacgaatgt ctcggatgct ctcgtcgaag
    25261 ccacctccga tctggtcttc gatacggctg gggaagagtc cgagcagtgg cagtccgtcc
    25321 agaccgcgct cgaaaaggcg caaagcgcga gcaagacttt tcagaaccct gagcatctcg
    25381 cctacatcga gcgtctgcaa cgagcagccg aagcgctgcc cttggttggt aagacgagct
    25441 cgatcgccga cgtcgtcaag accgtttatc gcgagctcat cagcggcgaa gacaaagact
    25501 atcggattcc cgaaagcaac gagggagtcg cccagaccct ccttcagttc cagtcatctc
    25561 accgcccgaa cgatctatgg catctggtga gccccgacta ccgcgagagc gcaatctgga
    25621 tgcagctcaa gagcggcgac aatcaggaca tgatgtccgt caaggccgag ctcgatgcgt
    25681 tcctttccgc gaatccgccg cctgacggac tgcgcgcaca atgggccggc ctgacctatc
    25741 tcaacgtcgt ttggcaggac gagatggtcg gcggaatgct cgaaagcctg atgggcgcgt
    25801 tcgtcatcgt cttcctgatg atggtgatcc tgttccgctc gcccgtgtac ggcgtgctcg
    25861 ccatgatccc cctgacgatc acgatcttgt tcacctacgg catgatcggt ctcgtgggta
    25921 aggactacga catgccggtc gccgtactct catccctcac gctgggactc tccgtcgact
    25981 tcgcgattca cttcctcgag cgggcgcgtt ccatccaagc cgtgaccggc gactggaagg
    26041 aaacgatggc gctcatgttc caggagccgg ggagggcgat cacacgcaac gcgatcgtca
    26101 tcgccatcgg cttccttcca ctgctcgccg cgccgctcgt tccgtacaac acggtgggct
    26161 tcctgatggc cgcgatcatg gccgtctcga gcatcgtcac ggttctgctg ctgccctcgc
    26221 tcatgatgca aatccaaaac tggctctctc cccggaagga gaaagtgtca acgaacgcag
    26281 aggacgcctc cgcgtcgctc gcgaccaaca aggagcaaac gatatgagaa ccctcagcaa
    26341 gacctcattg gcggccattg ccgccgcgat tctcttctcg accccgacgc tgtccgccga
    26401 ggccggggac ctcaaagacg cgagcgagat cgtgaaccgc acgaacagcg cgcagtacta
    26461 cgcagccaat gacgggcggg cgctggttcg gatgaagatc gtcgatgaca aggggcgctc
    26521 gcagcgtcgg cagttcgctg tcctccgtcg cgacgtgaaa gatggagccg atcagaacta
    26581 tctcgtcgtc ttcgatcagc ccgccgacgt tcgaaacacg gcgtttctgg tgaagaagca
    26641 cctgaataag gacgacgacc gatggctcta cttgccgggc ctcgatctcg tgaagcgaat
    26701 cgcggcgagt gacgagcgta ccagcttcgt tgggacccac ttcttctacg aagacatttc
    26761 gggacgccat cccagcgagg acacgcacca gttgctcgag acgacggaca ccgaatacgt
    26821 cgtcaagagc acgccgaaga gcagcaaagg tgtcgagttc gcgcactaca ccctgcgcat
    26881 cgacaagaag acatttttgc cgagcaaggt cgagtacgtg aatgcgcagg gcaaagtcta
    26941 ccggcgcatc gaaacggtca aagtggagga catgcagggt cacccgacga tcactcgact
    27001 caaagtgagt gacctcgact ccggtggata cacgctgagc gacgtaagca acgtgcaata
    27061 cgatctcggg atccctgacg acattttcgt cgagacctcg ctccgcaatc cgccgcagaa
    27121 gtggctccgc tgagcggagc gcgtgaagac acgaggggaa ccaggtgatg aaaccgacga
    27181 tctgtgcaag caagcgatgc cgagccgggc gaggatccct aaggtgtttt gcggtcgccg
    27241 cgctcgtttt cgtggctctc gccttcgcct cgggtgcctt cgctcaaggt ccacgggacg
    27301 gcgccccgcc tgtcggcgat gaggcgagcg gagaacccga agaggagccc gccagttcgt
    27361 cgaggtcgga gccgccttta gacccctcgt cagaagaggc cctcgaagcc ggggcggagg
    27421 ggccggtcga tgcggcgaaa accacgaaga ctccagagga gctcgctggg gacgtcgaac
    27481 caagcgatga ggccggccct gatgaagtct gggacgagtt cgatgactgg gaagaggaaa
    27541 agggagatga ctctggtgga aaagcctggg actttcagat cgggggattc gtcgagggcc
    27601 tcatcgcgcc tcgcgtggtt caaagcagcg cgagcgagaa cgagttcgta gccaacgagg
    27661 ctcgctttcg actcaacctc gacgccgtac acgagattgc cagcgcgcga ttccgcggcg
    27721 atgtctatgc agatgcggtc acgcgaacga tttggttcga cattcgagac gcgtccgttt
    27781 tcctgcgcgg tggcaactgg ttcaacatga ggctcggacg tcaggtgctc acctggggca
    27841 cgggtgactt cgtcttcttg aacgacctct tccccaagga cttcaactcg ttcttcatcg
    27901 gacgcgccga cgagtacctc aaggcgccct cgaactcgat catcacgacc ttcaacatca
    27961 aaaaggtcgg gctcgatctc gtctggacgc cgatcttcga gccggaccgc ttcatcgatg
    28021 gcgaacgttt gtcgtttttc aatcccttgc ttggcgagat caccggcgat cgttccgact
    28081 tcaccccaat cgatcccgtc tatccttcac agcgactcaa gaacggaacg tttcacggac
    28141 gcctgtacgg caccgcaggt gcatacgagc tcgcggccta cggctatgca gggttctgga
    28201 tgcagcccac ggcgctcgat ctcagcgagc tccaaccgag gccgacctat gcgcgacttg
    28261 ccgtctacgg ggccagtgct cgcggtccct tgttcggcgg cctcttcaac gtcgaggctg
    28321 cattctacga ttcgttcgat gattgggaag gaaccgaccc gacgattccc aattcgcagt
    28381 ttcggggcct cgtaggctat gagcacgagc tcttcccgaa gttccaactg ggtctgcagt
    28441 attacctcga atacacgttc aagtatgacg agctgatcgc gaactcactc tgggctgact
    28501 acgagcagaa cgagttccga catctcatca cggttcgact cacccaattt ctcgtgatgg
    28561 acaagctcga gctctcgctg ttcgtcttct tctcgccaga cgagctcgac acgtacatcc
    28621 ggccgcgcat cacctacaag atcatcgatc ccctcgcgat cgtcatcggt gcgaacctca
    28681 tgttcggtcg cgatgactac agcttcttcg ggcagctcga gcagaacacg aacgtctacg
    28741 cgagacttcg ctattcgttc tgagaattca acaaggaaag gaactggaag atgaacatcg
    28801 acaaactggt atttcgcgtt gcgggaatca tggtcaccac aggcgtggta ctcgcctaca
    28861 gccaccacat cgcctggctc gccctgccgc tcttcgtcgg tctgaacatg tttcaggcct
    28921 cgttcacggg attctgtccc atggcgacga ttgcaaagcg cttcggcatg accgagggca
    28981 aggcgttctg agcaacgaac cggatgcgct cgtcagtgtc ggccgtccga atggcagtcg
    29041 aggcaaaaat tgggaggcgc cgaaccggtg ggatagttgc gcacctcgcg gtgcttcggg
    29101 tccatctttg ctcgcgtgtg ctcgccagca tgacacccca cacaatcggt gttctgacca
    29161 gcaacgggtg agccgagcgc aacgttgtgg caagatgcgc agtcgttccg gtatctcgag
    29221 tgcgctcccg attggattgg gaaagcactt tcaggatggg cgccgcccgt ggccggaagc
    29281 caggctgagg tcgcatggca acccaggcag tcgcccgagg cggggtgtgt cgacgtgctg
    29341 ggccgttgcc attcgtgaca gtcgtagcag cgagtcggct tcgggacgtg cgcaaaggcc
    29401 gtctgcgtcc aatccggata cgagtggtgg caggtcgcac actcagggtc gtacgtttgc
    29461 gaatggaatg ggctgttcaa cctgggttcg tttccgtgac atccgatgca ggcggccgga
    29521 agtggcgtat gaatcgctac cggatcggcg agtgcccagt cgttcacgac gtgacagccg
    29581 gcacagtccc cgcggcctgc ggtcgtttga tgggttggag tcgagggtcg cagatcgctg
    29641 tggcaagtat tgcaggtgct cggtacaggg gtgtgtgcga agtcggtttg tgtccagtcc
    29701 ggatagttcc ggtgacagct cgcacattcg cgctgagtga cctgagtgtg gaagatcgtc
    29761 ggctgccgcg gttcgttctg gtgacaggtt aggcaggatg ccggaagcgg cgtgtggagt
    29821 gtggcggggt cggctgcgct ccagttggtg acgaggtggc atgaggcgca atcgccctgg
    29881 ccggccgcgg tttggtgcgt ggctgtgttc ggtctccgat cggagtgaca gtccaggcag
    29941 ctgctgggta ccggcgtgtg gaggaagtcg acctgcgtcc aatccggata gtcccggtga
    30001 cagctggcgc attcgggctc gaagatctga gcgtggaaga tcgtgggctg ccgagcctcg
    30061 ttttgatgac aggtccggca agacgcagga agtggtgcgt ggagcgcggc cgggtcggtg
    30121 acggtccagt cgtccgcgac gtggcaacgg gcgcagtccc cgaggccagc agccgattga
    30181 tgtgtcggcg tcgcgggtcg tagatcgctg tggcacgcgt tgcacgtgcc cggtgctggc
    30241 acgtggagga agtcggctgg tttccaatcc ggatagctcc ggtgacagct gcggcactcg
    30301 gagtcgaagg cctgggtgtg gaaaatcgtt tgcggttgcg gggtgttttg gtgacaggtc
    30361 ctgcacgacg ccggaagcgg cgtatggatt gctgccggat cggttgcggc ccagtcatcc
    30421 acgacgtgac agctggcgca gtcgccgcgg cctgcggtgg attgatgggt tggattcgtc
    30481 ggtctcagat cgctgtggca ggcgttgcac gtgctaggtg caggggagtg tgcgaagtcg
    30541 gtttgcgtcc aatccggata gttcgtgtga cagctggcgc attctgtctc ggcggcttgg
    30601 ctgtggaaga tcgtttgcgg ttgctggtcg ttttcgtggc acgtcacgca ggatgtcgga
    30661 agcggggtgt ggagcgccgc cgggtcggtt acggcccagt cgctgactat gtggcacgag
    30721 gcgcaatcgc cctgaccctg cggagtttgg tgcgttggcg tgtcgggtct ccgatcggag
    30781 tgacagtcta agcacgcgcg cggtgctggt gtgtgaagga agttcgcttg cgtccaatcc
    30841 gggtagttcc ggtggcagct gacgcagtcg gtgtccacga cctggatgtg aaaggcggtc
    30901 tccggtcgtg ggtcgttttg gtgacaggac acgcagcttg ccggaagcgg agtgtggatc
    30961 gtggtcgaca ccgctggact ccactggcgt tcggtgtggc agtcctggca ggtcgttggg
    31021 aggctgtcgt gcggtggact cagcgctccc ttgagatctg cttcatgaca cgtgacgcat
    31081 tgggcttgaa cgatggtc
//
LOCUS       DASRZF010000308         4903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1612533, whole genome shotgun sequence.
ACCESSION   DASRZF010000308 DASRZF010000000
VERSION     DASRZF010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 4903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4903
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1612533"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VFG22_13535"
     CDS             <1..236
                     /locus_tag="VFG22_13535"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HET6417312.1"
                     /translation="TFAVGAAAPLLTAFMSPTERIPMVVSFTSLGFLALLGGVSARTG
                     GAPVGRAVARVTFWGALAMAATAGIGALFGVAA"
     gene            393..2348
                     /locus_tag="VFG22_13540"
     CDS             393..2348
                     /locus_tag="VFG22_13540"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET6417313.1"
                     /translation="MTIRRPRRQTLWATACVLCAAALSFLAYESSANLSSEFAHELVA
                     KLSGTVYFLAVAFGGFFVYLVASLEDAPFTCRAAAALATPFLWMTKESVRLLESHPLS
                     ECLYYFLNPLNLWLLLLVVLEMAAATLVALRLRPNLSEPGRVLTLAPLMTIAVSLSLF
                     VGFYAWGRGENIYVQFLSGYRGLFGAGVAVPWDADDGVKRDIAGDHEAVSSRAKQAHH
                     PDDQPAPSGRPDIVFIVGDNHNAETMSCAGHPIIETPALDRLAREGVRFENAFNTTSL
                     CSPSRASILTGAYAHSHGVKNNHTPWTGEMPTFLEHLSNAGYATAFIGKWHMPGDGLP
                     EMPFLDLFVSYTYREGQGAYFDCPMVVNGREIASRKPYITEEVTDYAIEFIERAVAEP
                     AEERRPFAVYLAHRPGHPPYQAPAGIDGMYDEADVRETLPPNVDAWWFGKTNGNVFQG
                     TMMGSYFDQYRGYLETLTAMDEDIGRLLERIDELGLSENTVVVYMGDNGQQWGTHGRH
                     GIREPYADSARLPLIVRAPWLIPDPGTRREQIALNIDIAPTLLEVAGLDPPHHVDGES
                     LVPILRNGIAKSRDAFLMEFWRYFPENTPSYRGVRTQTHMYIEFEKGREPWLFDIARD
                     PDELHNLYTTPEGNQVLPDLLELLERL"
     gene            complement(2633..2917)
                     /locus_tag="VFG22_13545"
     CDS             complement(2633..2917)
                     /locus_tag="VFG22_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417314.1"
                     /translation="MSGITRTTRDHETIRKWTEARGGQPAHVPGTERTGAGVIRLQFP
                     ESEGASDLEPIGWDHFFEKFDEKRLALVYQEATSDGHVSRFNRIVESPTT"
     gene            3356..4087
                     /locus_tag="VFG22_13550"
     CDS             3356..4087
                     /locus_tag="VFG22_13550"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET6417315.1"
                     /translation="MTTTLYQFALCPFCNKVRAGLQLKGIPFEEVEVSPRSKVELPPL
                     PEGAPKKVPVLEVGDTVLWDSTQILSQLDEAFPETVRLTPTDEATRASADAMEQWVDE
                     EFIQSLPPVLYGTWREASKASKIIAQHSRFGAGEGLAVKLGGPVIMHAVAKRILKRNG
                     RTDAHAWVRENLDYFEQALGERDFVCGDVLSVADVAMHGALTCVSAFPIFENVQSRPR
                     LYAWFERVEAMKEPTADVAQQSSGC"
     gene            complement(4267..4521)
                     /locus_tag="VFG22_13555"
     CDS             complement(4267..4521)
                     /locus_tag="VFG22_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417316.1"
                     /translation="MPSPGYADVGELLRRLVDAGVEFIVVGGAAAVLHGAPITTEDLD
                     IVHRRSPENVSRLKTLLDELDARVRELANRRLRPQESALR"
     gene            complement(4499..4681)
                     /locus_tag="VFG22_13560"
     CDS             complement(4499..4681)
                     /locus_tag="VFG22_13560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417317.1"
                     /translation="MSDEPEEAPDVEELVRAAAREVDRALIQWALSFSPLDRLRASMR
                     SAASLQAFRDAKSRVR"
BASE COUNT          944 a         1532 c         1486 g          941 t
ORIGIN      
        1 cgacatttgc ggtgggcgcc gcggcgccgc tcctgaccgc cttcatgtcg ccgaccgaac
       61 ggatcccgat ggtcgtctcc ttcacgtctt tgggttttct cgctttgctc ggtggggtct
      121 ctgcgcgtac cggtggtgcc ccggtcggaa gagccgtggc tcgggtcact ttttggggcg
      181 cactggcgat ggcagcgacc gctgggatcg gcgccctttt cggagttgcc gcgtaaggcc
      241 tcggagatcg ggagttgcgg gggcctgcta cgcacagcgt tgttcggcgg aatttggggt
      301 gccgatggag gcggtgatgg ctgcgacgcg cgcgttccta aaacgattca agaacgcgcc
      361 tggacctccc ccatctggta caccctgtag caatgacgat tcggcgtcct cgaaggcaaa
      421 cgctgtgggc caccgcctgc gtcttgtgcg cggctgctct ctccttcttg gcttacgagt
      481 cttccgcaaa cctgagcagt gagtttgcac acgagctcgt cgcaaagctc tccggtaccg
      541 tttacttcct cgcggtcgca ttcggggggt tcttcgtcta tctggtcgca tcgctcgagg
      601 atgcgccctt cacttgcaga gcggcggccg ctctcgccac accgtttctc tggatgacga
      661 aggaatccgt tcgcctgctg gagtctcatc cgctgagcga gtgcctctac tacttcctca
      721 acccgctcaa cctctggttg ctgcttctcg tcgtgctgga aatggcggcg gcgacactcg
      781 tggcactaag gttgcgcccg aaccttagtg agccagggcg cgtgctcacg ctggcgccgc
      841 tcatgacgat cgccgtcagc ctctcactct tcgtagggtt ctacgcttgg ggccgtggcg
      901 agaacatcta cgtacagttt ctctcaggct accgtgggct cttcggcgcc ggagttgcgg
      961 tcccttggga cgcggacgat ggagtaaaac gcgacatcgc gggagatcac gaggcggttt
     1021 cgagccgcgc gaagcaggca catcaccctg acgaccagcc cgccccaagt ggccgccccg
     1081 acatcgtctt catcgtaggc gacaaccaca acgccgaaac gatgagctgc gcggggcatc
     1141 ccatcatcga gactccggcc ttggaccgcc tggcgcgcga gggcgtgcga ttcgagaatg
     1201 cgttcaacac gacctcgctg tgcagtcctt cgcgcgcgag cattctcacc ggcgcatacg
     1261 cgcactccca cggtgtcaag aacaaccaca ctccttggac cggcgagatg cctaccttcc
     1321 tcgaacacct gagcaacgct ggctacgcca ccgcattcat cggcaagtgg cacatgccgg
     1381 gggatggcct gcccgaaatg ccattcctcg acttgttcgt ctcctacacc taccgcgagg
     1441 gtcagggagc ctacttcgac tgccccatgg tcgtgaatgg gcgcgagatc gcctcacgca
     1501 agccctacat caccgaggaa gtcaccgact atgcgatcga attcatcgag cgggcggtgg
     1561 cagagcccgc cgaagagcgt cgtccgttcg ccgtctacct tgcgcaccgc cccggccatc
     1621 ctccctacca ggcgcccgcc ggcattgacg gaatgtacga cgaggccgac gtccgagaga
     1681 cccttcctcc gaacgtggat gcgtggtggt ttgggaagac gaacggcaac gtgtttcagg
     1741 gcacgatgat gggctcgtac ttcgaccagt accgtggata tctggagact ctcactgcga
     1801 tggacgagga catcggccgg ctgctcgagc gcatcgacga gctcggtctg agcgagaaca
     1861 ccgtcgtcgt ctacatgggc gacaacggcc agcagtgggg aacgcacggc cgccacggca
     1921 tccgcgaacc ctacgcagac agcgcgagac tgcccttgat cgtgcgcgct ccgtggctca
     1981 tcccggaccc cggcacgcgc cgcgagcaga tcgccctcaa catcgacatc gccccgacgc
     2041 tcttggaggt ggccggcctc gatcctcccc atcacgtcga cggagagagc ctcgtgccga
     2101 tcttgcgcaa cggcatagca aagagccgcg acgcgttctt aatggagttc tggcgctact
     2161 tcccagagaa cacgccttca taccgcgggg tgcgcactca aacgcacatg tacatcgagt
     2221 tcgaaaaggg acgggagccc tggctgttcg acatcgcgag agatccagat gagctgcaca
     2281 atctctacac tacgccggag gggaaccaag tcctcccgga tttgctcgag ttgctcgaac
     2341 gtctgtaggc cggaaacggg ttgtagaggg tctccccgaa ggggggcggc gtggtcccaa
     2401 agtgttccaa gaagccggcc ttgaccatgg ttgtcggggc ccgctacgca cagcgttgtc
     2461 cagtggagtt tgggacgaag atggaggcgg tgattgctgc atgagcaccc gaaaacaggt
     2521 agaaaatgct gagcgcaaac ctgcaaatct accaaagaac atcgcgcgtg cggaacaccg
     2581 ttcactcaac aacccgtggg tgcgttcaag gcggcgcgcg tacgcgcttt gtctaagttg
     2641 tcggagattc gacgattcga ttgaaccgac ttacgtgtcc gtcgctggta gcctcttggt
     2701 acacgagcgc gagtcttttt tcgtcgaact tctcaaagaa atggtcccag ccgatgggct
     2761 cgaggtcgct cgctccctcg ctctcgggga actggaggcg gatcactccg gctccggttc
     2821 gctcggttcc tggcacgtgc gcaggctgac cgccccgcgc ttccgtccat ttacgtatgg
     2881 tctcgtggtc tctggtcgtt ctcgtaattc ctgacatgga tctgaatttc ctttcgtgtc
     2941 gctgacgagg gccggagcgc tcccttcgga gcgcgctata ggcgtcgcaa agattcgtcg
     3001 ctcgaacctc atgaggtgcg tcgactcggt gaacatcgac cgttgcagtc gcccgcgcac
     3061 atcttcgccc gtccgttcaa tgggaacacg acgaggtgca ctcagcaggc tctcccccaa
     3121 gtccgcgtga gcataaaaac taccacattc gcagcaaaaa cacaagttct tgggggagga
     3181 acagatcgac cgaggttgcc cagaaagtcg gaatgtcacg caagcgcgtc ggctctttga
     3241 ccgataagcg tgccccgcat gtacgtcgcg gctcacgtct cgcggcggac gacgttttga
     3301 ggaacgaccg gggcttgcac caagtccaac gaccccgtag gccgagcgca agccgatgac
     3361 cactaccctt taccagttcg cgctctgccc cttctgcaac aaggttcgtg ctggacttca
     3421 gctcaagggc atcccgttcg aagaggtcga ggtgagcccc cggtccaagg tcgagttgcc
     3481 tccgctaccc gaaggggcgc cgaagaaggt cccagtcctc gaggtcggag ataccgtctt
     3541 gtgggactcg acgcagattc tatcgcagct cgacgaagca tttccggaaa cagttcgtct
     3601 caccccaacc gacgaagcca cgcgtgcgag cgccgacgcg atggaacaat gggtggacga
     3661 ggagttcatc cagtctctcc cgccggtgct ctatggaacg tggcgcgaag ccagcaaggc
     3721 ctccaaaatc attgcgcagc acagtcgctt tggcgctggc gagggtttgg ccgtcaagct
     3781 tggcggtccg gtgatcatgc atgcggtcgc gaaacgcatc ctcaagcgga atggacgcac
     3841 cgacgcacac gcttgggtcc gcgaaaactt ggactacttc gagcaagcgc tgggagaacg
     3901 agatttcgtt tgcggcgatg tcttgagtgt cgctgacgtt gcgatgcacg gcgcactcac
     3961 ctgcgtcagc gcgtttccga tcttcgaaaa cgtccaatcg cgacctcggc tctatgcatg
     4021 gttcgagcgg gtcgaggcga tgaaggagcc aactgccgac gtcgcccagc aaagcagcgg
     4081 atgttgaaag aggaatgatg aagtcgatgg ccgctcgacg aaccgtcaga agcaccctca
     4141 gggcaccgag caaaaagaaa cgcccctagg ggctcggggt gccgatagag gcggtccttg
     4201 gctcgcccgg ttttggtctt gagctctatc aacgttggca agtccaacac gaggaattcg
     4261 gcgccctcat cgcagggccg attcttgtgg gcgcagccga cgattggcaa gctcacgtac
     4321 gcgggcgtcc agctcgtcta gcagggtctt gagccgggaa acgttttcgg gacttcttcg
     4381 atgaacgatg tcgaggtcct cagtcgtgat cggtgcgccg tgaagcactg cagcagcgcc
     4441 gcccactacg atgaactcca cccccgcatc cacgaggcgt cgaagaagct cgccaacgtc
     4501 agcgtaccct ggacttggca tctctgaagg cctggagcga agcggcggaa cgcatcgaag
     4561 cacgcagtcg gtccagcggc gaaaacgaaa gcgcccattg gatcagcgcg cggtcgacct
     4621 cgcgcgcagc cgcccgtacg agctcttcga catcaggcgc ttcctccggc tcgtcagaca
     4681 cccgtggagg ctacaccgct tgcgccgcgg gagccaaccg ggggaccttg tcgccggccg
     4741 atcgagtacg ctggcactga atccggcaca gacgggaaaa ggccggctcg aaagagccgg
     4801 ctttgaccaa ggttgcaggg gccggatgtg aaccgacgac cttcgggtta tgagttcagg
     4861 aagcccggtt ttcacagtcc attacaacac atccgaatca ctc
//
LOCUS       DASRZF010000309         3373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1265020, whole genome shotgun sequence.
ACCESSION   DASRZF010000309 DASRZF010000000
VERSION     DASRZF010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1265020"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VFG22_13565"
     CDS             complement(<1..329)
                     /locus_tag="VFG22_13565"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET6417318.1"
                     /translation="MIDSLYAIIDLDAADHPLELTFKALNAGCAFVQLRAKHADDATF
                     LAIAKRMRSACTHAEVPFVINDRADIARLVHADGLHLGQDDLSVREARCVVGEMKIGR
                     STHDLDQA"
     gene            complement(322..1185)
                     /gene="aguB"
                     /locus_tag="VFG22_13570"
     CDS             complement(322..1185)
                     /gene="aguB"
                     /locus_tag="VFG22_13570"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR03381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0050126 - N-carbamoylputrescine amidase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HET6417319.1"
                     /translation="MTASSISIGIVQCALGGSREANVDRIESLIRDAATRGAKIILTP
                     ELFEGPYFPQSEDEHAFGLAAPLEGHPTVARMRDLARELDVVLPVSFFERAGQVYYNS
                     IVTIDADGTELGVYRKSHIPDGPGYEEKFYFRPGDTGFRVWKTRHGAIGVGICWDQWF
                     PEAARSMALQGAEILLYPTAIGSEPTAPEEDSAAPWRRVMLGHAVANAMPVAAANRIG
                     VEGDLTFFGTSFVCDQRGEIRAELGRDEEGVATVAFDRESLGRYRANWGFFRDRRPEL
                     YRKLTGDDPVD"
     gene            complement(1182..2180)
                     /locus_tag="VFG22_13575"
     CDS             complement(1182..2180)
                     /locus_tag="VFG22_13575"
                     /inference="COORDINATES: protein motif:HMM:NF016274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HET6417320.1"
                     /translation="MKERLRLPAEWEPHAACWLGFPHLEEEWPFNLPDAQRSVAALCQ
                     SIADAGNEPVNLLVQNDRLEERARSLIGDLRNVEYVRTDYGDCWLRDTAPLLGHTSSR
                     SLGALCFEFNGWGGKYLMPSDDRVRGWLTDRLAARRFDCDLFLEGGALESNGSGTILT
                     TASCALNENRNPGLTRDAFEKALGELVSMERLVWLERGLEHDHTDGHIDMIARFVATD
                     TVASMRSRPGAPNAGVLASIEQQLLASGFEVLSLPAPPEVKAPDGAPLPASYCNFYIA
                     NEAVIVPTYQVPEDELAIKELANAFPRREVIGLPARDLLWGGGVFHCVTQAQPVAP"
     gene            complement(2190..3170)
                     /locus_tag="VFG22_13580"
     CDS             complement(2190..3170)
                     /locus_tag="VFG22_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417321.1"
                     /translation="MPLLLLIWPLVGASQQAPDSFLGEPDAQRIESLRSAEIVEVKKG
                     KGGRSLAFQITLADGTQGYFKPQQTFSAAHWYSEIAAYYLDRELGLGRVPPTTGRRFQ
                     WSQLRAAANGDERISELGIAPDGTIKGAFIWWIPEPLKRVRMGRNWERWIRVQSALPI
                     TPYQRPVDYRSDLNHRPGIREATDTRRPIAQSPDTEERPAELSDLIVFDYLTQNVDRW
                     GGDFTNVRSRGEGGPLVYLDNGAGFWLGNQRLGLMEARLKAVQRFRRSTVEAVRSLDI
                     ERFSKRLSADPLAPVLNDKQLDGLAQRRRALLEHVDAMIARFGEARAVPW"
BASE COUNT          639 a         1070 c         1029 g          635 t
ORIGIN      
        1 gcctgatcga ggtcgtgagt agagcggccg atcttcattt ctcccacaac acaccgagcc
       61 tctcgtacgg aaaggtcgtc ctggcccaga tgcaagccat ctgcgtggac gaggcgcgcg
      121 atatcagcgc ggtcgttgat cacgaaggga acttcagcgt gggtgcacgc agatcgcata
      181 cgcttcgcga tcgccaagaa ggtggcgtcg tctgcgtgct tggcccgaag ctgcacgaaa
      241 gcgcagccgg cgttcaacgc cttgaacgtg agctcgagcg gatgatccgc agcatcgagg
      301 tcgatgatgg cgtagagcga atcaatcaac ggggtcgtct cccgtcagct tgcgatagag
      361 ctccggacgg cgatctcgga agaaccccca gttcgcccga tagcgtccga ggctctcccg
      421 gtcgaaagcg acggtcgcca cgccttcctc gtctcgaccg agctccgctc gaatctcgcc
      481 ccgctggtca cagacgaacg aggtaccgaa gaaggtgaga tcgccttcta cgccgattcg
      541 gttggccgca gcgacgggca tcgcgttggc cactgcgtgc cccagcatga cccgtcgcca
      601 gggcgctgcg ctgtcctctt caggtgcagt tggctcgctg ccgatcgccg tcgggtagag
      661 caaaatctcc gcaccttgaa gcgccatgct ccgcgcggct tccggaaacc attgatccca
      721 gcaaatgccg acgccaatcg cgccgtggcg tgtcttccaa acgcgaaacc cggtgtcgcc
      781 aggacgaaag tagaatttct cctcgtagcc cggtccgtct ggaatgtggc tctttctata
      841 aacgccaagc tccgtaccgt cggcgtcgat cgtcactata ctgttgtagt agacctgccc
      901 ggcgcgctcg aaaaacgaga ccggaaggac gacgtcgagc tctcttgcga ggtcgcgcat
      961 tcgcgccacg gtcggatgcc cctcgagtgg tgccgcgaga ccgaacgcgt gctcatcttc
     1021 cgactgcgga aagtatgggc cttcgaaaag ctcgggcgtg aggatgatct tcgcaccgcg
     1081 cgtggcagca tcgcgaatca aggattcgat gcggtcgacg ttggcttctc tgctgcctcc
     1141 gagggcgcat tgaacgatgc cgatcgagat ggatgaggcg gtcatggtgc gacgggctgg
     1201 gcttgtgtga cgcagtgaaa gactccgcct ccccatagca ggtcccgtgc cggtaggccg
     1261 atgacctctc gtcgagggaa cgcgttggcg agctccttga tcgcgagctc gtcctcaggg
     1321 acctggtagg tgggcacgat caccgcctcg ttcgcgatgt agaaattaca ataactcgcc
     1381 ggcagagggg ctccatcggg agcctttacc tccggcggcg ctggaagaga gagcacctcg
     1441 aacccgcttg cgagcagctg ctgctcgatc gacgcgagaa ctcctgcgtt gggggcccct
     1501 ggcctgcttc gcatcgacgc aaccgtatcg gtcgcgacga accgcgcaat catgtcgata
     1561 tgtccgtccg tgtgatcgtg ctcgaggcca cgctcgagcc atacgagtcg ctccatcgaa
     1621 accagctcac cgagggcctt ctcgaaggcg tcccgcgtca atccggggtt tcggttctcg
     1681 ttcagtgcgc atgaggctgt cgtcaagatg gttcccgaac cgttcgattc gagcgcgcca
     1741 ccctctagga acagatcgca atcgaatcgt cgcgcagcga gtcgatccgt cagccatcct
     1801 ctgactcgat cgtcggatgg catcaggtac ttcccccccc agccgttgaa ctcgaagcaa
     1861 agggctccga gtgacctgct cgaggtgtgc ccgagcagag gcgccgtgtc gcgcagccag
     1921 caatcgccgt agtctgtcct cacgtactcg acgttgcgca gatcaccgat caacgagcga
     1981 gcccgctcct cgagtcgatc gttctgcacc aacaggttca ccggctcgtt cccggcgtcg
     2041 gcaatgcttt ggcaaagagc cgccacggag cgctgcgcat cgggaagatt gaatggccac
     2101 tcctcctcga gatgaggaaa tccaagccag catgccgcgt ggggctccca ctcggccgga
     2161 agccgaagtc gttctttcac ggccggaatc taccacggca cggcccgcgc ctcaccgaag
     2221 cgagcgatca tcgcgtccac gtgctcgagc agcgcgcgcc tgcgctgggc gaggccgtcc
     2281 agctgcttgt cattcaagac cggagcgagg ggatcagcac tcaaacgctt tgaaaaacgc
     2341 tcgatatcga ggctccgcac ggcttcgacc gtcgaacgcc gaaaacgctg cacggccttc
     2401 aaacgcgcct ccatgaggcc gaggcgctgg ttcccgagcc agaaccctgc accgttgtcc
     2461 aagtagacga gaggcccgcc ctctccgcgc gaacgaacgt tcgtaaagtc tcctccccag
     2521 cggtcgacgt tctgtgtgag gtagtcgaag acgatcaaat cggagagctc cgcgggccgt
     2581 tcctcggtgt ccggggattg cgcgatagga cgccgcgtgt cggtggcctc acggatcccc
     2641 gggcggtgat tgaggtcgga gcgatagtcc acggggcgct ggtacggtgt gatcggcaga
     2701 gcgctctgaa cacggatcca acgctcccag ttgcgtccca tgcgaacgcg cttgagtggc
     2761 tcggggatcc accagatgaa tgcacccttg atcgtgccgt caggcgcgat tccgagctcg
     2821 gagattcgct cgtcgccatt tgcagcagct cgcagctggc tccattgaaa cctgcgcccg
     2881 gtcgtcggtg gaacgcgacc gagaccgagc tcgcgatcca agtagtaggc cgcgatttcg
     2941 gaataccaat gcgccgctga gaacgtctgc tggggtttga agtagccctg cgttccgtct
     3001 gcgagtgtga tctggaaggc aaggctgcgg ccccccttgc ccttctttac ctcgacgatt
     3061 tccgccgatc ggagcgactc gatcctttga gcatccggct ctcccaggaa tgaatccggc
     3121 gcctgctgag aagcgccgac aagcggccaa atcagcaaca gcaatggcat gaatggagcg
     3181 cctcgatgca ccgcgccagc ctactgctca atgttcagaa accccatttt tgcgctagaa
     3241 ggggcgtccg agggttctcg ccactggtcc ggtttcccta gaccttggtg gacgcgtcga
     3301 gtcctaggag atcgatctcg cgtcccaggg atcgcgccct agcctcgtcg tagatcgcgc
     3361 gtgcaaggga tac
//
LOCUS       DASRZF010000310        28446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4705093, whole genome shotgun sequence.
ACCESSION   DASRZF010000310 DASRZF010000000
VERSION     DASRZF010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 28446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28446
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4705093"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..438)
                     /locus_tag="VFG22_13585"
     CDS             complement(130..438)
                     /locus_tag="VFG22_13585"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HET6417322.1"
                     /translation="MPIHGGSGRFMFSVISSRFVQHVGYADIPTGNSYIQTVTWDETE
                     CPDAYAVLTYSQSTNPASAHYADLTQVYSDKSWNDMPYCDADIQAAKIDEIDISNLEN
                     "
     gene            complement(527..976)
                     /locus_tag="VFG22_13590"
     CDS             complement(527..976)
                     /locus_tag="VFG22_13590"
                     /inference="COORDINATES: protein motif:HMM:NF019473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="limonene-1,2-epoxide hydrolase family protein"
                     /protein_id="HET6417323.1"
                     /translation="MQSTAEVRADGTSAKSAEEVVRAFVAALERLDLDSAVSLAAPKI
                     RWVNVPWKTATDKARFKKVLEGMFKDAKRFEVQYFDIHERGDGVVYTDRVDIFEGGGL
                     TMHLPVQGEFRVQDGLITEWVDRFSWAKLVADMGRSLPGIVKYRLGR"
     gene            1050..2294
                     /locus_tag="VFG22_13595"
     CDS             1050..2294
                     /locus_tag="VFG22_13595"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET6417324.1"
                     /translation="MLAKFVILQSPMWVLPRSRLLLSLLVSMLAFLAACAHLPKRSPL
                     PAEEIDAAQVLGIPRARMWGDQPPPWEHEWIEKSKTELKERYSGVYGRPQTYLAISGG
                     GENGAFVAGLLQGWSESGDRPVFTTVTGISAGALVAPFAFLGSEYDEVLRRVSMDLRP
                     EDVYKKRGMIRALRTDSLATTEPLEALIAKYVDEEVMEKIAAAHRDGRVLNIGTANLD
                     SMRPVIWRIGAIANSGHPGALALIRKILLASASVPAAFSPVLIQVEVDGKTYDELHVD
                     GGAASQVFLYPVGLDYEMVLEKLEVPGRPKVYVIRNSRLEPIYKQVPNRLFPLAGRSL
                     ESLVRTQGIGDLYRIYLETCRDGLDFNLAYIPSDFTEEPKSEFDTEYMKKLYDLAYER
                     AKNGYAWDKMPPELERAPLQCR"
     gene            2345..2791
                     /locus_tag="VFG22_13600"
     CDS             2345..2791
                     /locus_tag="VFG22_13600"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET6417325.1"
                     /translation="MRTLFLSLALMVFVLGCKDASPAAATEPGPSDAPAVKAEISEIS
                     VDDASKALESGAIAVDANSASTREKNGTVPDAIILTSSYKYDLAQLPEDKSKDLIFYC
                     SNTNCTASDAAAERASSNGYEKVSIMREGIKGWKQAGKPTKPYPQS"
     gene            complement(2837..4237)
                     /locus_tag="VFG22_13605"
     CDS             complement(2837..4237)
                     /locus_tag="VFG22_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HET6417326.1"
                     /translation="MHVHLIGVAGTGMSALAALLVEAGHRVSGSDIAFDPPVGPYLES
                     LGIECLRGWQASNLDSRPDLVIVGNVCRPDNAEAREAIKREIKTVSMPGALAENVLLE
                     RKPLVVAGTHGKTTTSTLLAYLLRQTGSDAGFFIGGIPLNFDRSSRLGAVGAPFVIEG
                     DEYDSAFFEKVPKFWSYRPSAAILTSIEYDHVDIFPDEAAYLAAFRRFVELIPKDGWL
                     FAWAGDPRVREVAADAHCKVRFYALDGDECGDVEPMWLGIRAPGGAIDLFGGGSLCGR
                     ASLPLRGRHNARNTVAALAMGSEAAGAPLDRLIAALPGFRGSKRRQELVGITKGIEVY
                     DDFAHHPTAVKETLLAFRERAGGRLIAVFEPRSATASRRLHEEAYPDAFVSADVSILA
                     PVGRSEIPADEKLDIAAIAEAITRSGRKAHACATVDEIIDAAAAEAKPGDTIVVMSNG
                     RFEDAPDRILLALVER"
     gene            4321..4470
                     /locus_tag="VFG22_13610"
     CDS             4321..4470
                     /locus_tag="VFG22_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417327.1"
                     /translation="MEKKTKNLVDRVRDVMDTLVEALEGLLNPPPALVPVRVRPQYPA
                     IKRRR"
     gene            complement(4478..6658)
                     /locus_tag="VFG22_13615"
     CDS             complement(4478..6658)
                     /locus_tag="VFG22_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HET6417328.1"
                     /translation="MTSTHYRTCNLCEAMCGLEVELDGAEVVSIRGDERDVFSRGHIC
                     PKATALKDLYDDPDRLKQPVRKVGDRWEPISWDEAFDMAVDRIHQIQEKHGRSAVGLY
                     FGNPNAHNFGTMLYGPAFFRALGTKNRFSASSCDQWPLMLASYFMYGHQLLFPVPDVD
                     RTDFMMLIGANPLASNGSIMSAPGIKKRLEGIQERGGKVIVVDPRRSETARIADEHIF
                     IQPGTDALFLLGLLHEVCASGVDLGRLEPHVKNLDRLLDIAKAYPPESSESITGVPAA
                     TVKRLAREMQAAPKAVAYGRMGACTQEFGGLNMWLVNAVNAATGNLDEPGGSMFATPA
                     IDTLKKIGGFGIGRGSYGRWRSRVRGLPEFGGELPSSVMAEEILTEGEGQIRAMVTLA
                     GNPILSTPNGGRLDQAFESLEFSLAIDFFINETSRHADLILPPVSPIQRSHYDLALYL
                     VAVRNTANYSAPPFTNSKDELDDWQILSELSCRMAAKRHGKLSKEYLSIRAVQKAGPE
                     RVLDLGLRLGPYGKRLNPLGTGLSLGKLRKEPHGVDLGPLQSALPDRVPEAHGAIDLA
                     PDLFVEDLERLRARFAAEALPTPEGKLLLIGRRHLRSNNSWMHNTARLMKGKLRCTLM
                     MNPDDAAKIGVQSGDTVTITSRVGEVSAKAEVTSDMMQGVVSLPHGFGHGRDGVRLSV
                     ASGHPGVSVNDLTDDQFIDLISGNAAFSGVPVTVQAAEQIAAAE"
     gene            complement(6704..8911)
                     /locus_tag="VFG22_13620"
     CDS             complement(6704..8911)
                     /locus_tag="VFG22_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6417329.1"
                     /translation="MKKLTLRLDSYDDDARRYVAAAQQLADERRHAEVETIHLWYTLV
                     DESPVAQSALEAAGVEPRNAIVEAEWALRRLARTSEGVEAYLSSGFLELLSRAELEAT
                     RHAGLPVTPADLLLACAQEPAGLVRLVLRASGLSSAILRESLPARTRSAGATPVGDGG
                     ILEVYGRDLTRLATQDAFDPFVGRDAELRRMLQVLARREENNPLLVGEIGTGRTALVE
                     ALASRIAAGEVPEMLADKRIIALDAGSLVAGTKLRGELEERMRAILDAVRDSGGRVIL
                     FLPNLASFLRAKGGAGDMLASALSRGEVHALARCTPDELREIDDDASALSRRFVSIQV
                     EEPSADEALAILEGVRPRFESAHGVRIADQALEAAVRFARRYVAGRVLPKSAIDLIDE
                     SAARVRLGLDGDGSDQAVGPEDVAAVVSLWTGIPAAKMMEEEAEKLLHMESRLRERVV
                     GQEQAVSAVSKAVRRGRVGLRDPKRPIGSFLFLGPTGVGKTELAKALAEFLFDDEAAL
                     TRLDMSEFMEKHMVARLLGAPPGYVDSEAGGFLTEAVRRRPYSVVLFDEIEKAHPDVF
                     NILLQVLEDGRLSDSRGRVARFHDTVIIMTSNVGSELILEHDGSDTALREAIQDRLRD
                     YFRPEFLNRVDEILIFDRLDKDAIRQILGIQLGQIRALLVQQELELKLTDAAKTRLID
                     LGYEPAFGARPLKRVLVRELQDPLAEALLEGRFTAGDTIKVDSNGVSFSFESG"
     gene            complement(8908..9189)
                     /locus_tag="VFG22_13625"
     CDS             complement(8908..9189)
                     /locus_tag="VFG22_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HET6417330.1"
                     /translation="MNEDDEAPPHIDFNTFILSLSTSALMHLGDLPGAEEDSTLGLAH
                     AKQSIDCIALLEAKTRGNLTGEEERLITEVLYDLRLRFVKAKKAADSKA"
     gene            complement(9195..9374)
                     /locus_tag="VFG22_13630"
     CDS             complement(9195..9374)
                     /locus_tag="VFG22_13630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417331.1"
                     /translation="MATLGNKLMDKRIVERNIAKGLVTKEEYEQHLANLADCEGDYER
                     VEIDPGDSTDQSRAE"
     gene            9545..11017
                     /locus_tag="VFG22_13635"
     CDS             9545..11017
                     /locus_tag="VFG22_13635"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6417332.1"
                     /translation="MRELEILIRAYHPLLLVDTLEEERVHALLEHAAERTSLPLFSWT
                     QVDGLRRELPDPGQTPETGPPQKALAFIERSNLEAIFHLRGLGPYLHEPAIIAQIKNI
                     HKKYFGHRGALVVSGHGIDMPPSIEHLFTPMNLHAPSAEAYHSFVQAVLRDLAKRLHV
                     KVQLSSADVSKLLSALHGLTFFEVQKIITQAVIEDGVLGREDIVKVLDAKRQIIERSG
                     VLEYFPHEHDMGEVAGLGELKAWLRKRQAAFEDPKRAKEYGLTPPKGLMLIGVQGCGK
                     SLCAKAVAAEWKLPLIRLDPSNLYNRYVGESEKNLKRAIKLAEAMAPVVLWIDEIEKA
                     LSQEDAEGGPAQRIFGTFLAWLQDKKESVFVIATANDISKLPPELMRKGRFDEIFFVD
                     LPTGEAREKIIRIHLERRKRDPAKFDIARLARISDGFSGAELEQAVVSALYTAFSKQE
                     DLTTEGVVEEIEMTKPLSVTMAERIDSLRAWAKDRAVFAG"
     gene            11084..12835
                     /locus_tag="VFG22_13640"
     CDS             11084..12835
                     /locus_tag="VFG22_13640"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HET6417333.1"
                     /translation="MRRCPFLAIAALAVFLSACGSEATSVAGSGGSGGNDSARGPVVD
                     TDSGPVAGVENDGVISYRGIPYAAPPVGDLRWQPPVDPSRWADVLQATEKPSICPQES
                     FAGLPVPGFMPSEDCLYLNVDAPVEGSDLPVMVWIHGGGFTLGEGVQTDGGTAGDLIA
                     REAGLVVVSMNYRLGQLGFLAHAALSDESADGASGNYGLMDQAAALRWVRQNIAAFGG
                     DPTNVTIFGESAGAFSVCSHLASTSSAGLFEKAILQSGSCERPWPTLAAAEAQGTAFA
                     AALGCDSEDDVLGCMRAKSADEVLAALPPAPNFGFSPSDEASGRWGPVFDGFFFAEQP
                     RDSFVSGEFNQVPTLIGFTREEARLFVWLGQISDPPLEVTADNYISLIAYFLGGDQEL
                     AASAAEEYPLEDYAEPALALAAVATDMIFRCPGKLEATKLSGYVPTHLYQFEYPDGHS
                     QLEVALPFIGASLPSYDLAAFHGSDIPYVFGYDPLLEIDFENFATTLNSWAPDTRDEA
                     LWLEMLGYFARFAASGDPNVEGAVDWPVYDPETDRHLVLDAEPSLGENAAPNCTFWEG
                     EDYLVPELSLEPLLSLQ"
     gene            complement(12839..13324)
                     /locus_tag="VFG22_13645"
     CDS             complement(12839..13324)
                     /locus_tag="VFG22_13645"
                     /inference="COORDINATES: protein motif:HMM:NF022161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 domain-containing protein"
                     /protein_id="HET6417334.1"
                     /translation="MPHLTINYAQDIDTVYRFVTDPERIKKRCEASGEKNVRIQVEES
                     GGTLVITSTREIDSDLPGFAKKLFSATNTIVERREWRDAGDNKTCRSHIDVVGTPGKI
                     DSNISISPSGSGCTYDIEFEVTAKVPLIRKKLEEFVAKTTMEGLREEHEFNQRELNAA
                     G"
     gene            complement(13382..14764)
                     /gene="mgtE"
                     /locus_tag="VFG22_13650"
     CDS             complement(13382..14764)
                     /gene="mgtE"
                     /locus_tag="VFG22_13650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HET6417335.1"
                     /translation="MRLAPLIGPDLQDAIAIGPEAVREAVSEFHPEDIAELLEDLSAH
                     EAVVLVRALPTEAAADVVERLSPQRQVLIFSAIDTGRAVELLGEMDPDDRVDLLQELE
                     EEDARSLLAALERREPEAAEEVRELERYEPETAGGLMTTEFAALPPETKVWEAMEAAR
                     RFGREEMAEMLYYIYVCRPNGELLGVVSLRDLILSEPGQPLSEIMTENVFQVSAFDDQ
                     EKVAHAMARYDLAALPVVDNLSRMLGVVTIDDVVDVVIEEATEDAQKMGGVVPLEDSY
                     FATGWAEFVWKRGSWLVLLFLAQLLTATVIRENRDMLAATVELVLFIPLIIASGGNAG
                     SQSATLIIRAMAVGELRPGDWARVAGRELLIGLSLGIALGLIGFVRGWFAGETVAPLA
                     IATAVGLSILAIVTLSTMIGSLLPLLIRRAGLDPAVSSTPFIASVVDVLGLIVYFAVA
                     QVILHVVFGA"
     gene            complement(14814..15530)
                     /locus_tag="VFG22_13655"
     CDS             complement(14814..15530)
                     /locus_tag="VFG22_13655"
                     /inference="COORDINATES: protein motif:HMM:NF015686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HET6417336.1"
                     /translation="MLELVTNPDAWVALLTLTLLEIVLGIDNIVFIAILASRLPVEKQ
                     ALAYRLGLMGAMVTRIALLMTINWVMQLTQPLFEVVGHPFSGRDLILLGGGLFLIAKS
                     AQEIYNKVEGEHQGDIQGWFSGLPGVVGQIMVLDIIFSLDSVITAVGMAEHIEVMVTA
                     VVIAIGVMLLFAKQIGDFVNKYPSMKILALSFLLLIGVLLTAEGLGQHVNKGYIYFAM
                     AFALMVELLNIRFRSKREPA"
     gene            15615..16763
                     /locus_tag="VFG22_13660"
     CDS             15615..16763
                     /locus_tag="VFG22_13660"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HET6417337.1"
                     /translation="MAYRFATIAAHAGRLSERPGDPVTAPITLSTTFLQSGPGESEEH
                     AYSRVGNPSRDALERTLTELEAGSSAAVFSSGCAAMHAVLQLLRPGEQLIACGDVYGG
                     TYRLVEQLVKPMGVDVIWVDMCDLEAVDAAMNDRSKMLWIETPTNPLLRIFDITALSN
                     LAHARGAWCVVDNTFATPLLQRPLQLGADFVVHSMSKYLNGHSDVVAGAVISQAADLS
                     DRLRFIQRAGGAVPSPFDCYLVARGIKTLPIRMTRHCESAEALAQWLKQRAGVDEVHY
                     PGLEAHPQHELAARQMSAFGGMLSFEVSGGLERARSVLQRVRLFSLAESLGGVESLIG
                     QPALMSHASMPEDVRKTQGIRDGLIRVSVGLEDVEDLREDLDQALSTP"
     gene            16799..17407
                     /locus_tag="VFG22_13665"
     CDS             16799..17407
                     /locus_tag="VFG22_13665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417338.1"
                     /translation="MGLGMNMTRRSICVLLPLLALSCAEGTSPVTVDAQWNLTCPADS
                     EVGCGALAPETCLGPIGQRTIAGEFREISCTGDPIIAVCNSVERADGTTFIELEANVG
                     NDFAFELTAIVNRNDDSVGATCDVTIVEDQLPYDVGACGREPPSMDQPCQLTNISTDG
                     AEVSFDLQCASLLSSTSSLGFDVGAVGGGPTTIRFQNCTGSF"
     gene            complement(17404..18942)
                     /locus_tag="VFG22_13670"
     CDS             complement(17404..18942)
                     /locus_tag="VFG22_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HET6417339.1"
                     /translation="MAQFALKTALPNYLRDEQQRIEAFARTVGLDFFPTIFEMLSYDQ
                     MNEVAAYGGFPTRYPHWRWGMEYERLSKSHEYGLSKIYELVINNNPAIAYLLEGNSMV
                     DQKLVMAHVYAHVDFFKNNFCFRMTSQGRDSRTSADVRKWIDTMANNGSIIRKWANRI
                     GIETVEQFIDACLSLENLIDPQKPFLPKDLTPSTSEEKEPPEKPELQLLRVDRDYMES
                     FINPEEFVESQRKKLEEEAEQAQRFPREPARDVLGFLLENAPLQRWERECLAVVRTEA
                     YYFLPQMQTKIMNEGWASYWHSRLMTENICDASEIVDYADRCASVLATAPGQLNPYKL
                     GIELFRHIEDRWNKGQFGKEWDDCDDWELRRHWDRRTGLGREKIFEVRALYNDVTFID
                     EYLTEDFVFDQKMYSFGYNQRNDRWEIESRTFENVKTQLLTSLTNAGNPLISVVDANH
                     GNRSELLLEHTHQGADLRLDWAKEVLKALSRVWTRPVEVHTVVDEKPTALRFDGKDHS
                     QKPL"
     gene            complement(18946..20055)
                     /locus_tag="VFG22_13675"
     CDS             complement(18946..20055)
                     /locus_tag="VFG22_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HET6417340.1"
                     /translation="MSLRIDQDHSRFRNIVRGRIRQNLRKYISKGELLGRQGKDIVSI
                     PIPHIDIPKLRFGDKQQGGVAQGDGDPGDPLSGSPQEGDGQGKAGDQAGDHPLEVELT
                     LDELADILGEELELPNIENKGKSAIVDKKDRYVGVRNVGPNSLRHFKRTFRSALRRQI
                     ALGTYDPKRPVIIPTRDDMRYKSWKTEEEPVANAVIIYMMDVSGSMGDEQKEIVRIES
                     FWIDTWLRKQYKDIETRFIIHDAAAREVDRDTFFRTRESGGTMISSAYRLAADMIEQN
                     YPSEQWNIYPFHFSDGDNWSVDDTLLCVDILKKLLLPASNVFCYGQVDSPYGSGQFIK
                     DLKEHFSGDEQLITSEINDRDAIVESIRVFLGKGK"
     gene            complement(20059..22104)
                     /locus_tag="VFG22_13680"
     CDS             complement(20059..22104)
                     /locus_tag="VFG22_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase"
                     /protein_id="HET6417341.1"
                     /translation="MGEMVKQIAALQDYESYQDLHWEGSFEDYLQIVREKPQVTRNAF
                     QRLYDMVVSYGEEEYIDNKKRLIRYPFFKDPIENGKDAIFGLDIPLMRLVHVLHAAAQ
                     GYGPEKRIILLHGPVGSSKSTIARLLKKGLERYSRTAEGALYTYEWTNLHQTGLAGGD
                     DVFPCPMHDEPLRLIPTEWRERAVQELRLGDDRRRIQVPGELNPACRFIFRSLLERYN
                     GDWSKVMANHIKVKRLILSEQDRVGIGTFQPKDEKNQDSTELTGDINYRKIAEYGSDS
                     DPRAFNFDGEFNIANRGVVEFVEVLKLDVAFLYDLLGATQEHKIKPKKFAQTDIDEVI
                     IGHTNEAEYKKLINNEFMEALRDRTIKIDIPYITKMSEEIKIYQKDFSSERLRGKHVA
                     PHTLEVAAMWAVLTRLEEPKKHQLALLQKLKLYDGKILPGYTQDNVKELRKEANREGL
                     EGMSPRYVQDKISNSLVRDGNEGYINPFMVLNELEKGLSASLTVADEEGKRYAELIGV
                     VKAEYDEMVKNEVQRAISADEEAIGRLCANYIDNIRAYLMKQKVKNKYTGKDEEPDER
                     LMRSIEEKIDITDPRKDDFRREIMNYIGHLALEGKKFTYETNDRLRRALEMKLFEDQK
                     DSIKLSSFVSNVIDKETQDKIDIIKNRLIKYYGYNEASATDVLAYVASIFARGDVKE"
     gene            complement(22156..22917)
                     /locus_tag="VFG22_13685"
     CDS             complement(22156..22917)
                     /locus_tag="VFG22_13685"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET6417342.1"
                     /translation="MVEELSYQSLAILAAAGVLATVVNVMAGGGGMIVLPALMALGLP
                     ADVANGTYRLAVVTQSIAGTSAMHRAGKLETGSVVPILIPTTLGAVLGALLATWVPRE
                     VLKPIMLVTMIVMAALIAFRKQTLIPDDGPILRPNQAPKSYAGLFLAGVYGGFIQAGV
                     GFLLLGVLIGTLRHSLITGNALKLVVTLAFGTVSLGIFAWAGLVSWTPAIVLAAASIV
                     GARIGVRLMLKVPAAGLRWFVFACVVATCIAAWLR"
     gene            22996..24318
                     /locus_tag="VFG22_13690"
     CDS             22996..24318
                     /locus_tag="VFG22_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET6417343.1"
                     /translation="MKKTYVLDTNVVLHDPLAVYKFEDNAVVLPIFVIEEVDQFKKEL
                     SELGQNARHLSRILDDLRNQNGHTLDQGVPLASTGGELRVAVPPRMPKRASGPGEMDR
                     FILEMALELRDENPEQPLVFVTMDTNLRIRADALGLKAETYEGGRVAIDDLYSGVITV
                     DGDRELVDNLARREPVEASALGVELYPHAGIVLREHDRPRHTALGRFDPKSGNIVPLR
                     VGREGVWRVRPRNLEQYFALDMLLSPDIHLLTLVGKAGTGKTLLAIAAGLQSVVQEGT
                     YSRILVSRPIFPMGRDVGYLPGTVEEKLNPWMQPIFDNLEYIFSSGRGRLSEHRNYGD
                     LISSGVIQVEPLTYIRGRSLPNQFLVVDEAQNLTPHQVKTVITRCGADTKIVLTGDPY
                     QIDNPYVDAASNGLTVVAQRFQNESIGAHVTLSKGERSELAERATQLL"
     gene            24284..25522
                     /locus_tag="VFG22_13695"
     CDS             24284..25522
                     /locus_tag="VFG22_13695"
                     /inference="COORDINATES: protein motif:HMM:NF019626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine/phenylalanine carboxypeptidase
                     domain-containing protein"
                     /protein_id="HET6417344.1"
                     /translation="MSSQSARRNFFERPRVRLRAELDVALRRLHGKARLLVGSTTHSD
                     PAERHRLCQCVARGEHAKPRWEWRPVAIERSVWRELARARELAEASEAAALYLPRLEE
                     LEIELLLLESLGRSKQVRPMAARLFGTGSERLFPDSNHSILDAAHEILSNASLEQEEK
                     TIPAASVEGVNLRDLMLAYGKHVGLHIAVRIDPGLIANAAVGERTVFIADRCFGAHEA
                     QRLATHEVYGHLVSGFNGRTQTLGIFSIGTAGSYGDQEGVAIYLEELAGLLDSSRQRT
                     LAGRLLATHAMHAGVSFGDAARVLVSEHDFSPEAAVTLCERAFRAGGVARDAVYLTGW
                     LRVRRAVARGETNLAELQLGKVSLSALPELRRLARDGLLTQPVYLSNLAKSLGSTGAG
                     TRLATLPPSLATSLTRLDAT"
     gene            complement(25414..25908)
                     /gene="coaD"
                     /locus_tag="VFG22_13700"
     CDS             complement(25414..25908)
                     /gene="coaD"
                     /locus_tag="VFG22_13700"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HET6417345.1"
                     /translation="MRGIAIYPGSFDPITKGHTAILKSGLVAFEKIIVAVLNNDNKKP
                     LFSVAERMEMIRESADDMGVEPGRIDVDNFDGLLVGYARRRNARIILRGLRAVADFEY
                     ELQMANMNRHLSPEIETVFIMANDAYFYVASNLVKEVARLGGNVASLVPAPVLPRLLA
                     KFDK"
     gene            complement(25905..26531)
                     /gene="rsmD"
                     /locus_tag="VFG22_13705"
     CDS             complement(25905..26531)
                     /gene="rsmD"
                     /locus_tag="VFG22_13705"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HET6417346.1"
                     /translation="MRLWRVWAPGAEVMRIVGGRLSGRRFSAPLGRGTRPTSDRVREA
                     LGSALEARNAFGDAQVLDLFAGTGALSFEALSRGAKHSVLVEQDPGAIRQINQSAKEL
                     GLAGKIRTIRLDLLADPSGVIRKLPATESSFTLLFADAPYSDTDSVSALLNALTASGR
                     LAPGALLVVEHSATHDWRWPKGLAPEADYRYGQTGISLGVYAPGKGPQ"
     gene            26742..>28446
                     /locus_tag="VFG22_13710"
     CDS             26742..>28446
                     /locus_tag="VFG22_13710"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET6417347.1"
                     /translation="MRKICLRCGSKFGSRAEYCPHDGSLLVTDDEIPDPLIGTVLLEQ
                     FRIDEQIGTGGMGTVYRANQTTVGRDVAIKVLRAELARDEQAVFRFEREARVAISLDH
                     PNLVRVFLSGRLKDGRLYIVMELLEGRSLADELDQEGVPSLERVLIMIMKLCAGLGAV
                     HAAGIVHRDIKPENVYLVRRGRDNDFVKLVDFGIARILAGEGIGPTTTQSGRVFGTAT
                     YISPEAATGEETDQRSDIYSMGVLSYQLLTGELPFEGKTAGAVLMQHVHQEPPLLQTV
                     GRGVEVPDDLAGVVMRALSKDPDARQQSLAEFLNALSQAAGEAGLLNDARALLFGTIW
                     GEDLAAPGSYLAELLGSSAPPGSPSALAETQPAQLGRDPKEEALLSSAPKPFGTTAYD
                     LVAPVRKANGRKHRWLWASLGLLCLIMIGLVVGAWWLQRPPRATIDETIAEPVLAPPA
                     VEIGVSNETEQQKPPVEKAVPDEPVLEAKVEPNPKKKKRTTTKRTGVEAGTAAAAAAA
                     AATEIGTAAETGTGTETGTGTGTGTETATGEGAAPDDGIDWSVPDFPQEPPAAANAAP
                     SE"
BASE COUNT         5313 a         8796 c         8596 g         5741 t
ORIGIN      
        1 tccgctggcg cgccggcatc cggctcgtcg gccagcgtag ccgaggcgca cacgagcaca
       61 aggatcacga ggatcgctgg ggccagggaa cgcatcggcc gcagaatgcc agccgatgag
      121 ggttcggagc tagttttcca agttgctgat gtcgatttcg tcgatcttcg cagcctggat
      181 gtccgcatcg cagtacggca tgtcgttcca gctcttgtcg gagtagacct gggtcaggtc
      241 cgcgtagtgg gccgacgcgg ggttcgtcga ctgcgagtag gtcaacaccg cgtatgcgtc
      301 tggacattcg gtttcatccc aggtgacggt ctggatgtag ctgttgcccg tgggaatgtc
      361 cgcgtagccc acgtgttgga caaagcgcga gctgatcacg ctgaacatga accgtcccga
      421 gccaccgtgg atcgggatgt cctccccggt ctaagtcgct cggtcacccg aacgaggccg
      481 cgaccctacc aggagccacc gattcgtgca gtcgcttttt ccggggctat cgcccgaggc
      541 gatacttgac gatcccgggc aggctcctgc ccatgtcagc gacgagcttc gcccagctga
      601 atcggtccac ccattcggtg atgagcccgt cctggacgcg gaactccccc tgcacgggga
      661 ggtgcatcgt taggccgcct ccctcgaaaa tgtcgactct gtccgtgtaa acgaccccat
      721 ctccgcgctc gtggatgtcg aagtactgaa cctcgaatcg cttcgcgtcc ttgaacatcc
      781 cctcgagcac cttcttgaac cgggctttgt cggtagccgt cttccaaggg acgttgaccc
      841 agcggatttt cggcgcggcc agtgacacgg cggaatccaa atcgagccgt tccagcgcgg
      901 caacaaaagc cctcacgacc tcctctgccg acttcgcgct cgttccgtcc gcccggactt
      961 cagctgtgct ttgcatcata gactaactat aggggcatcg cgtgtggcga aaaggggcga
     1021 tggtcgaatc ctttgggcgc ggagctccca tgctcgcaaa gtttgtgata cttcagtcgc
     1081 ccatgtgggt tctgccgcgg tcccgactgc tcctctcgct actggtatcg atgctcgcgt
     1141 tccttgcggc ctgtgctcac ttgcccaagc ggtcgccact ccccgctgag gagatcgacg
     1201 cagcccaggt gctcggaatt ccaagggccc gcatgtgggg ggaccagcct ccgccgtggg
     1261 aacacgagtg gatcgagaag agcaagaccg agctgaagga gcggtactcc ggtgtttacg
     1321 gccgtcctca gacgtacctg gcgatttcag gcggcggaga gaacggtgcc ttcgtggccg
     1381 gacttcttca gggctggagc gaatctggag accggcccgt tttcacgacc gtgacaggca
     1441 tcagcgccgg cgcgctcgtc gcaccttttg ccttcctagg ttccgagtat gacgaggtat
     1501 tgcgacgcgt ctcgatggac cttcggcccg aggacgtcta caaaaagcgc ggcatgatcc
     1561 gagcgcttcg gacggactcc ttggcgacca ctgaaccgct tgaggccttg atcgcgaagt
     1621 atgtcgatga ggaggtcatg gaaaagatcg cggcggctca tcgggacggc cgcgttctga
     1681 acatcggcac tgcaaatctg gactcgatgc gaccggtgat ttggcgcatc ggcgccatcg
     1741 ccaacagcgg ccatccgggc gcgctggctt tgattcgcaa gatcttgctg gcctcagcgt
     1801 ccgtgcctgc tgcgttctcc ccggtgctga tccaggtcga agtggatggg aagacctacg
     1861 acgagctgca cgtcgacggt ggagccgctt cgcaagtgtt cctctatccg gtggggctcg
     1921 attacgagat ggtcctcgag aagctcgagg tcccgggtag acccaaggtc tacgtcatcc
     1981 gcaactcacg gctcgaaccg atatacaagc aggttccaaa ccggcttttt cctctcgccg
     2041 gccgttcgct cgagtccttg gttcgaactc aggggatcgg agacctctac cgtatctacc
     2101 tcgaaacctg ccgcgacggt ctggacttca acttggccta cataccctcg gacttcaccg
     2161 aggagccgaa gagtgagttc gacaccgagt acatgaagaa actctacgat ctggcctacg
     2221 aacgggcgaa aaacggctac gcttgggata agatgccccc cgagctcgag cgcgcgccgc
     2281 tccagtgccg atgacgccgc aagatggcgc ttgctggtcc gcgcgccggc tctaagctcc
     2341 aaagatgcga actctcttcc tctccttggc cctcatggtt ttcgtgctgg gctgcaaaga
     2401 tgccagcccg gccgcggcca ccgagccggg gcccagcgac gccccagccg taaaggccga
     2461 gatttcagaa atctccgtcg atgatgccag caaggcgctc gagtcggggg ccatcgccgt
     2521 ggatgcaaac tccgcttcga cgcgcgaaaa gaacggcacg gttcccgacg cgatcattct
     2581 cacctcgtcg tacaagtacg acctggcgca gctccccgaa gacaagagca aggatttgat
     2641 cttctattgc tcaaacacca attgcaccgc ctccgacgcc gccgccgaac gcgcctcgag
     2701 caatggctat gaaaaggtca gcatcatgcg cgaaggcatc aagggctgga aacaagccgg
     2761 caagccgacc aagccgtacc cgcagagcta agggttctcg cgcgaagggt ccgttgggtg
     2821 cagctcgccc ctggcctcag cgctctacga gcgcgagaag gattcgatcc ggggcgtctt
     2881 cgaatcttcc gttcgacatc actacgatcg tgtcgcctgg ttttgcttcg gctgcggccg
     2941 catcgatgat ctcatccacg gtcgcgcaag cgtgcgcttt ccttccgctt cgcgtgatcg
     3001 cctctgcgat cgctgcgatg tcgagcttct catccgctgg gatttcactc cgcccaaccg
     3061 gggccaagat gctgacgtca gcggacacaa aagcgtctgg atacgcctcc tcgtgcaagc
     3121 gcctcgaggc ggtcgcgctt ctaggctcga acacggcgat gagcctgcca cctgcacgtt
     3181 cgcgaaatgc gaggagtgtc tctttcacag cggtcgggtg atgcgcgaaa tcatcgtaga
     3241 cctcgatgcc cttggtgatg ccgacgagct cttgccggcg cttgctcccc cgaaagcccg
     3301 ggagtgcagc gatcagccga tcgagcggtg caccggccgc ttcactcccc atcgccaaag
     3361 cagccaccgt atttcgcgca ttgtgacgtc cgcgaagcgg taagctcgca cgaccacaaa
     3421 gcgacccgcc gccgaagagg tcgatcgcgc cccctggggc ccggattccg agccacatcg
     3481 gctccacgtc cccgcactcg tcaccgtcga gcgcgtagaa tcgaaccttg caatgcgcgt
     3541 cagcggcgac ctcgcggacg cgtgggtcac cggcccaggc aaacagccag ccatctttcg
     3601 ggatcagctc gacgaatctt cgaaacgcgg ccagatacgc ggcctcatcg gggaagatgt
     3661 cgacgtgatc gtactcgatc gaggtgagaa tcgcggcgct tggacggtag ctccagaact
     3721 tcggcacctt ctcgaagaac gcgctgtcgt actcatcgcc ttcgatcacg aatggtgcgc
     3781 ctacggcgcc aaggcgtgag ctacggtcga agttcaaggg gattccgccg atgaagaatc
     3841 cggcgtcgct ccctgtttgc cgaagcaggt acgccagcag cgtcgaggtc gtcgtctttc
     3901 cgtgcgtgcc tgccaccacc aggggcttgc gttccaagag cacgttttca gccagcgcgc
     3961 ccggcatcga aaccgtcttg atctcgcgct tgatcgcctc tcgggcctcg gcgttgtccg
     4021 gcctgcagac gttgcccaca atcacgaggt cgggtctcga gtcgaggttc gaggcctgcc
     4081 aaccccgaag acattcgatt ccgagcgatt ccaaatacgg accgaccggc ggatcgaatg
     4141 cgatgtcgct gccgcttacg cgatgccctg cttcgacgag cagcgcggcc agcgcactca
     4201 tccccgttcc ggcgacaccg atcagatgaa catgcatcta ggctccgcta acccacaatt
     4261 gccgagcaat atcgacccag gtgttgacac tgtcacgact gggcccatta tgaagtgtag
     4321 gtggagaaaa agacgaaaaa ccttgtcgac cgtgtccgcg atgtcatgga cacgctcgtt
     4381 gaagctctgg aaggtttgct caacccgcct cctgctctgg tgccggtgcg tgtgcgcccg
     4441 cagtaccctg ccatcaagcg ccgccgctaa ggggggctca ctctgcggct gcgatttgct
     4501 ccgctgcttg caccgtgacg ggcacgccgc tgaaggcggc attaccactg atgaggtcga
     4561 tgaactgatc gtccgtgaga tcgttcacgc tcacgccagg atggccgctc gcgacactca
     4621 atcgcactcc gtcgcgaccg tgaccgaatc cgtgaggtag gctcactacg ccttgcatca
     4681 tgtctgatgt gacctcggcc ttggcggaca cctctccgac acgcgaagtg atggttaccg
     4741 tgtcgccgga ctgcacgccg atcttcgccg catcgtccgg attcatcatg agcgtgcaac
     4801 gtagcttacc tttcatcaga cgcgccgtat tgtgcatcca ggagttgttg ctcctgaggt
     4861 gccggcggcc gatgagcaag agcttcccct ccggcgtcgg taaggcctcg gcggcgaacc
     4921 gagcacggag gcgttcgagg tcttcgacga aaagatcggg tgccaggtcg attgcgccat
     4981 gcgcttcggg gacgcgatcc ggcaatgcgc tttgcagcgg tccgaggtcc actccgtgcg
     5041 gttccttcct gagcttgccc aacgagagac cggtgccaag tgggttgagc cgcttgccgt
     5101 agggtccgag tcgaaggccc aggtctagca cgcgctcggg tcccgctttc tgaacggcac
     5161 ggatcgagag gtactccttg ctgagcttcc cgtgccgctt ggcggccatc cggcagctca
     5221 gctcgctcaa gatctgccaa tcgtccagct cgtccttcga gttggtgaac ggaggcgccg
     5281 agtagtttgc ggtattgcga acggcgacga gatagagggc gaggtcgtag tggcttcgct
     5341 gaataggaga cacgggcggc aggatcaggt cggcgtggcg gctcgtttcg ttgatgaaga
     5401 agtcgatagc gagtgaaaac tcgagcgact cgaacgcctg gtccagcctg ccgccattgg
     5461 gggtggacaa tatgggattt ccagcgaggg tcaccatcgc tcgaatctgc ccctcgcctt
     5521 cggtcaggat ctcctcggcc atcaccgagg atggaagctc accgccgaat tccgggagtc
     5581 cgcgtacccg gcttcgccag cggccgtagg agccgcggcc gataccaaag cccccgatct
     5641 tcttcagggt atcgatcgca ggcgtcgcga acatcgagcc accgggctca tcgagattgc
     5701 cggtcgctgc gttgacagcg ttgacgagcc acatgttgag cccgccgaac tcctgggtgc
     5761 aggcgcccat tctgccgtat gcgacggcct tcggagcagc ttgcatttcg cgcgcgaggc
     5821 gcttcaccgt cgccgcgggc acgcctgtga tcgattcact cgattcgggc ggataggcct
     5881 tggcgatgtc gaggaggcga tcgaggtttt tcacgtgcgg ctcgagccga ccgagatcga
     5941 cccctgaggc acagacctcg tggagcaggc ccagaaggaa gagcgcgtcg gtacccggct
     6001 gaatgaaaat gtgctcatcg gcgattcgtg cggtctccga gcggcgcggg tcgaccacga
     6061 tcacctttcc gcctcgctct tgaatcccct cgagacgctt cttgatgccc ggcgcggaca
     6121 tgatgctccc gttcgacgcc agcggattgg cgccgatcaa catcatgaag tcggtgcgat
     6181 cgacatcggg gaccgggaac aagagctggt gcccgtacat gaagtacgag gccagcatca
     6241 gcggccactg gtcacatgag ctcgccgaga atcggttctt ggtcccgagc gcacgaaaga
     6301 acgcgggccc gtataacatg gtgccgaagt tgtgagcatt ggggttgccg aagtagagac
     6361 ccactgcgct ccgcccatgc ttctcctgga tctggtgaat ccgatcgacg gccatgtcga
     6421 aggcctcgtc ccaggagatc ggctcccaac ggtcacccac cttgcgaact ggctgcttca
     6481 atcgatccgg atcgtcatag aggtccttca acgctgtcgc cttcggacag atgtgcccgc
     6541 ggctgaatac gtcccgctcg tcgccacgaa tcgaaacgac ttcggcgcca tcgagctcga
     6601 cctcgagccc acacatggct tcgcagaggt tgcaggttcg gtaatgggtg ctggtcatcg
     6661 gcgaagcata cccgagcatc ggctccaggc gtagccgcgg cgctcagccg ctctcaaacg
     6721 agaacgagac gccgttcgag tccactttga tcgtatctcc agcggtgaag cgaccttcca
     6781 gcaaagcctc cgccaaaggg tcctgaagct cccgaacgag gacccgcttg aggggacggg
     6841 ccccaaacgc gggctcgtaa ccaaggtcga tcagtcgtgt ttttgccgca tcggttagct
     6901 tcaattcgag ctcctgctgc acgagcagcg ctcgaatctg cccgagttga atcccgagaa
     6961 tctgccggat cgcgtccttg tcgaggcgat cgaagatcag gatctcatcg acacggttga
     7021 ggaactccgg tcgaaagtag tcgcgcaggc ggtcttggat cgcttcgcgc agcgcagtgt
     7081 ccgaaccatc gtgttcgagg atgagctcgc ttccgacgtt cgacgtcatg atgatcacgg
     7141 tgtcgtggaa gcgcgcgacc ctgccgcgcg aatcgctcag cctcccgtcc tccagcactt
     7201 gcagcaggat gttgaacacg tcgggatggg ccttctcgat ttcgtcgaag agcacgaccg
     7261 agtaaggcct acggcgtact gcctccgtta gaaacccgcc cgcttcgctg tctacgtacc
     7321 cgggtggcgc cccgagtagg cgcgccacca tgtgcttttc catgaactcg ctcatgtcga
     7381 ggcgagtgag agcggcctcg tcgtcgaaga gaaattcagc aagcgctttt gcgagctcgg
     7441 tcttaccgac acccgtaggt ccgaggaaca gaaacgagcc tatcggacgt ttgggatctc
     7501 ggagacccac ccggccccgc cgaacggcct tgctcacggc gctcactgcc tgctcctgcc
     7561 cgacgacccg ttcgcggagt cggctttcca tgtgcaggag cttttcggct tcctcttcca
     7621 tcatcttggc tgccgggatg ccggtccaga gcgacaccac cgctgccacg tcttcggggc
     7681 cgacggcctg atcggagcca tcgccgtcga ggccgagacg cacccgggcc gcgctttcat
     7741 cgatgaggtc gatggcgctc tttggaagta cgcggccagc gacgtagcgg cgcgcgaatc
     7801 gtacggcggc ctcgagggcc tgatcggcga ttcggacgcc gtgtgcagat tcgaacctag
     7861 gccgtacccc ttcgaggatc gccagggcct cgtccgcaga cggctcctcg acctggatcg
     7921 acacgaacct tcgtgacagc gcgctcgcat cgtcgtcgat ctcacgaagc tcgtctggag
     7981 tgcatcgcgc gagcgcgtgg acctcgccgc ggctcaatgc gctggcaagc atgtcgcctg
     8041 cgcctccctt ggcccgaaga aacgatgcca agttgggtag gaagagaatc acccgtccac
     8101 ccgagtcgcg gaccgcgtcg agaatcgctc gcatgcgttc ctcgagctca cctcggagtt
     8161 tcgtccctgc aacgagcgag ccggcgtcga gtgcgatgat ccgtttgtcc gccagcatct
     8221 cggggacttc gcctgccgca atgcgagacg cgagcgcctc gaccagagcg gtgcgccccg
     8281 ttccgatctc acctacgagc aaggggttgt tttcctctcg acgcgccaga acctgaagca
     8341 tgcgtcgaag ctcggcgtct cggccaacga agggatcgaa cgcatcctgc gtggccaggc
     8401 gtgtgaggtc gcggccgtac acttcgagga tgcccccgtc gcccaccggc gtggcccctg
     8461 cgcttctggt cctggccggc agagattcgc gcaagatcgc actgctcaag ccggaggcgc
     8521 gcaggaccaa ccgaaccagg cctgcgggtt cctgcgcgca tgccaggagc agatcggccg
     8581 gagtgaccgg caggccagcg tgccgggtcg cttcgagctc cgcgcgtgac aagagctcca
     8641 agaagcccga cgaaagatag gcctccacac cctcgctcgt tcttgcgagg cggcgcaggg
     8701 cccattcggc ctccacgatg gcattgcgag gctcgacccc cgcggcttcg agcgccgatt
     8761 gcgccaccgg cgactcgtcg accagcgtgt accagagatg gatcgtctcg acttccgcgt
     8821 gtcggcgctc gtccgcgagt tgctgcgctg ccgcgacgta tcgacgtgcg tcgtcgtcat
     8881 acgagtccag gcgcagagtg agcttcttca agccttggag tccgcagctt tcttcgcctt
     8941 cacgaaccga agcctcagat cgtagagaac ttccgtgatg agacgttctt cctcgcctgt
     9001 gaggttgcca cgcgtcttcg cttcgagcag cgcgatgcag tcgatcgatt gcttggcgtg
     9061 ggcaagaccg agggtcgaat cctcttcggc accgggcaga tcacccaggt gcatcagcgc
     9121 ggacgtgctg agcgacagga tgaacgtgtt gaagtcgatg tgcggaggtg cctcgtcgtc
     9181 ctcattcatg ggcgctactc cgctctggat tggtctgtag agtcacccgg gtcaatctcg
     9241 acccgttcat agtcgccttc gcagtcggcc aggttcgcga gatgctgctc gtactcttcc
     9301 tttgtgacca agcccttcgc gatgttccgt tccacgatgc gcttgtccat caacttgttt
     9361 cccaaggttg ccatgcggcc gagtgtagat tcgccccacc cgctgtcaac tcagccctcg
     9421 gtgcgcctgc caatgaggtg ccgagacccc acgcctgtcg cctgaggcac acaagtttga
     9481 cagtccccag cccacccccg atcctgaaat cgtgctcagg ggggttccac catcggctga
     9541 ccagttgcgc gagctggaga ttctcattcg cgcctaccat ccgctcttgc tcgtggacac
     9601 cctcgaagag gagcgggttc acgctttgct cgaacatgcg gcggagcgca cgagcctgcc
     9661 gctcttttcc tggacccagg tcgacggtct tcgtcgcgag ctgccggacc cgggccagac
     9721 ccccgaaacc ggaccgcctc agaaggcact ggcgttcatc gagcggtcga acctagaagc
     9781 gatttttcac ctgcgcgggc tcggtccgta tctgcacgag cccgcgatca tcgctcagat
     9841 caagaacatc cacaaaaagt acttcggtca tcgcggagcg cttgtggtct ccggtcatgg
     9901 catcgacatg cctccaagca tcgagcattt attcacgccg atgaacctgc acgcgccctc
     9961 ggccgaggcg taccattcgt tcgttcaagc ggtcttgcga gacttggcca agcgactgca
    10021 cgtcaaggtt cagctttcga gcgccgacgt gtccaagctg ctctcggccc tgcacgggct
    10081 cacctttttc gaggtgcaga agatcatcac gcaggccgtc atcgaggacg gggtgctcgg
    10141 gcgcgaagac atcgtaaaag tgctcgatgc caagcggcag atcatcgaac gctcgggcgt
    10201 tctcgagtac tttccgcatg agcacgacat gggggaggtc gccggcctcg gagagctcaa
    10261 ggcctggcta cggaagcgcc aagccgcgtt cgaagacccg aagcgtgcca aggagtatgg
    10321 actcactcca ccaaaggggc tgatgctgat cggagttcag ggctgcggaa agagtctttg
    10381 tgccaaggcg gtcgcggcgg agtggaagct ccccttgatt cgcctcgatc cttccaatct
    10441 ttacaaccgc tacgtcggcg agagcgagaa gaacctcaag cgggcgatca agctcgccga
    10501 ggccatggct cccgtggtcc tctggatcga cgaaatcgaa aaagccctct cgcaagaaga
    10561 tgccgagggc ggacctgcgc agcgaatctt cggcaccttc ctcgcatggc ttcaggacaa
    10621 gaaggagagc gtgttcgtca tcgcaacggc gaatgacatc tcgaagcttc ctcccgagct
    10681 catgcgaaag ggtcgcttcg acgagatctt cttcgtcgac ctcccgaccg gggaggcgcg
    10741 cgaaaaaatc attcggattc atctcgaacg caggaagcgg gatcccgcaa agttcgacat
    10801 cgcccggctg gcccggatca gtgacggatt cagcggcgcc gagctcgaac aagcggtggt
    10861 atccgcgctc tacacggcgt tctcgaagca agaggacctc acgaccgaag gggtcgtcga
    10921 ggagatcgag atgaccaagc cgctctcggt caccatggct gagcgaatcg actcgctgcg
    10981 cgcctgggcc aaggaccgcg ccgtcttcgc cggctgagcc cgcccggatg gcggccaggg
    11041 ccggcatgcc gtaggttccc gttcttggat tgagaggtct tggatgcgtc gttgcccgtt
    11101 tctcgcgatc gccgcgcttg cggttttctt gtctgcttgc ggtagtgagg cgacgagcgt
    11161 tgccggttcg ggtggctcag gcggcaacga cagcgctcgt ggacccgtcg tcgacaccga
    11221 ctcggggccg gtggcgggcg tcgagaacga tggcgtcatc tcctatcgcg gcatccccta
    11281 cgcggccccg ccagtaggcg atctccgatg gcagcccccc gtagatccct ctcgatgggc
    11341 agacgtgctc caagccactg aaaagccgag catctgccct caggaatcat tcgctggcct
    11401 ccccgtgccg ggcttcatgc caagcgaaga ttgcctctac ctgaatgtgg atgccccggt
    11461 cgaaggctcg gacttgccgg tcatggtctg gatccacggt ggcggcttca cgctcggaga
    11521 aggtgtgcaa accgatggcg gaaccgccgg tgacctgatc gcgcgtgagg ccggtctagt
    11581 cgtcgtgagc atgaactacc gacttgggca gctcggattt ctcgcgcacg ccgcgctcag
    11641 cgatgagagc gcggatggag cctccggcaa ctatgggctc atggatcagg ccgctgcgct
    11701 cagatgggtt cggcaaaaca tcgcagcgtt tggaggcgac ccgacgaacg tcaccatctt
    11761 tggcgagtcc gctggggcat tcagtgtctg tagccacctc gcctccacgt cgagcgccgg
    11821 acttttcgag aaggcgattc tgcaaagcgg ctcttgcgaa cggccctggc ccacgcttgc
    11881 tgcggccgaa gctcagggga cggcatttgc cgctgccctc gggtgtgact ccgaggacga
    11941 cgtgcttggc tgcatgcgtg ccaagtccgc cgacgaggtg cttgccgcct tgccgccggc
    12001 gcccaatttc ggcttcagcc cgagcgacga agcatcgggc agatggggtc cagtcttcga
    12061 tggtttcttc ttcgcagagc aaccgcggga ttcgttcgtc tctggtgagt tcaaccaggt
    12121 tccgaccctg atcggcttca cccgcgaaga agcgcgtctt ttcgtctggt tgggtcagat
    12181 ctccgacccg cccctagagg tgacggcgga caactacatt tcgctgatcg cctacttcct
    12241 aggcggagac caggagctcg ccgctagtgc agccgaggag tatcccctcg aagactatgc
    12301 agagcctgcg cttgcccttg ccgcggtggc taccgacatg atcttccgct gcccgggcaa
    12361 gctcgaggcc acgaagctct cgggctacgt gcccacgcac ctctatcagt tcgagtatcc
    12421 ggatggacac tcgcagctcg aagtggccct gccgttcatc ggtgcatcct taccgagcta
    12481 tgatctcgcc gcgtttcacg gttcggacat cccctacgtc ttcggatacg atccgcttct
    12541 cgagatcgac ttcgaaaact tcgcgaccac gctcaacagt tgggctcctg acaccaggga
    12601 cgaagcactg tggcttgaaa tgctcggcta cttcgctcgc tttgcagcaa gcggcgaccc
    12661 caacgtcgag ggcgccgtcg actggccggt gtacgacccg gaaaccgaca ggcatctggt
    12721 tctcgacgcc gagccatctc tcggcgagaa cgcggccccg aattgcacct tttgggaagg
    12781 cgaggactat ctcgtgccgg agctatctct cgaaccgcta ctcagcctgc aatgagattc
    12841 agccggcggc gttgagctcg cgctggttga actcgtgctc ttcgcgcagg ccttccatcg
    12901 tcgtcttggc gacgaactcc tcgagcttct tccggatgag cggaaccttc gcggtgactt
    12961 cgaactcgat gtcgtaggtg caaccgctgc cgctcggtga gatgctgatg ttcgagtcga
    13021 tcttacctgg tgttccgacg acgtcgatat ggctcctaca agtcttattg tcgccggcat
    13081 cccgccattc tcgacgctcg actatcgtat tcgttgcgct gaagagcttc ttcgcgaatc
    13141 ctggaaggtc gctatcgatc tcacgtgtgg acgtgatgac cagcgttccg ccggattcct
    13201 cgacttgaat gcggacgttt ttttcgcccg acgcttcgca acgctttttg atccgctcgg
    13261 gatcggtgac gaagcgataa acggtgtcga tgtcttgcgc gtagttgatg gtcagatgcg
    13321 gcatatcttg ctcccggtac gaagacgaga gcctaacacg cctgcggcga agatcgagag
    13381 gctaggcgcc aaacacgacg tgcagaatca cttgcgcaac cgcgaaatag acgatgagac
    13441 ccaatacgtc gacgaccgaa gcgatgaacg gcgtcgaaga gacggccggg tcgagacctg
    13501 ctcgtcgtat cagcagtggc aggagcgatc caatcatggt gctcaaggtg acgatcgcga
    13561 gaatgctcaa tcccactgcc gttgcgatcg cgagtggggc gaccgtttcg cctgcaaacc
    13621 agccccggac gaagccgatc agaccgagtg caatcccgag cgaaaggccg attaggagct
    13681 cccggcctgc cactcttgcc caatcgccag gccggagctc gccgactgcc atcgcgcgga
    13741 tgatcagcgt tgcagactgc gagccggcgt ttccgccgga ggcgatgatc agggggatga
    13801 agaggacgag ctcgaccgtc gctgcgagca tgtcacggtt ctcccgaatc accgtggcgg
    13861 tcagcagttg cgcgagaaag agaagcacga gccaggaccc gcgcttccac acgaactccg
    13921 cccatccggt tgcaaagtaa gagtcctcca gaggtacgac gccgcccatt ttctgtgcgt
    13981 cttcggtcgc ctcttcgatc acgacgtcga ccacatcgtc gatggtgacc acgccgagca
    14041 tgcgtgaaag attgtcgacc accgggagtg cggccaaatc gtagcgggcc atcgcgtgtg
    14101 cgaccttttc ttgatcgtcg aacgccgaaa cttggaagac gttctccgtc atgatctctg
    14161 agagaggctg cccaggctcg ctcaggatta ggtcccgcag cgagacgaca cccagcagct
    14221 cgccgttggg ccggcagacg tagatgtaat atagcatctc ggccatctct tcgcgtccga
    14281 agcgccgagc tgcttccatg gcttcccaga ccttcgtctc cggaggaagc gcagcgaact
    14341 cggtggtcat caaaccacca gccgtctccg gctcgtatcg ctcgagctcg cggacttctt
    14401 ctgccgcttc cggctcgcgt cgttcgagag ccgcaagcag ggatctggcg tcctcctcct
    14461 cgagctcctg aagcagatcg acgcgatcgt cggggtccat ttccccgagg agctcgaccg
    14521 cccttccggt gtcgatcgcg ctgaagatca agacctgacg ctgcgggctc aggcgctcca
    14581 ccacatccgc agccgcttcg gtcggcagtg cgcgcacgag caccacggcc tcgtgcgccg
    14641 acaggtcctc tagaagctct gcgatgtcct ccgggtggaa ctcggagacc gcctcgcgaa
    14701 cggcttctgg cccgatcgcg atcgcatctt gaaggtccgg tcctatgaga ggcgccaagc
    14761 gcatcgcggc tgattctgtc gcatgcggag agctgccgca gcaaaattct cgctcaagcc
    14821 ggttctcgtt tggagcgaaa tcggatgttg agaagctcga ccatcaacgc aaaggccatc
    14881 gcaaagtaga tgtagccctt gttgacgtgc tgcccgaggc cttcggccgt cagcaagact
    14941 ccgatgagca ggaggaagga caaggccagg atcttcatcg atggatactt gttgacgaag
    15001 tcaccgatct gtttggcgaa cagaagcatc actccgatcg cgatgaccac cgctgtgacc
    15061 atcacctcga tatgctccgc catgcccact gcggtgatca cggagtcgag cgagaagatg
    15121 atgtcgagga ccatgatctg tccgacgacg cccggcagac cgctgaacca gccctggatg
    15181 tctccctggt gctcgccctc gactttgttg tagatctctt gggcgctctt ggcgatgagg
    15241 aacaggccgc cgccgagcag gatgagatcg cgacccgaga acggatggcc gacgacttcg
    15301 aacaacggct gagtaagctg cattacccag ttgatcgtca tcagcagggc gattcgggtt
    15361 accatcgctc ccatcaggcc gaggcggtac gcaagcgcct gcttctcgac tggaagtcgc
    15421 gacgccagga tcgcgatgaa cacgatgttg tcgatgccca gtacgatttc gagcagcgtc
    15481 aacgtcagca acgcgaccca tgcatccgga ttggtgacta gctcgagcat gttcgcggcc
    15541 attcttagct tgactcaccc attcgccgca aacccctctt ggcagccgtc gtctgccgag
    15601 gtacgcccag gcctatggca tatcgatttg cgaccatcgc ggcgcacgcc gggagactca
    15661 gcgagcggcc cggcgacccg gtcaccgctc cgatcactct ttccacgact ttccttcaga
    15721 gcgggccggg agagtcggaa gagcacgcgt atagccgcgt cggaaacccg agccgagatg
    15781 cgctcgagcg caccctgacc gagctagaag cgggatcgag cgctgctgtg ttctcttccg
    15841 gatgcgccgc gatgcatgcg gtgcttcagc ttctacgccc aggagagcaa ctcatcgcct
    15901 gcggcgacgt ctatgggggc acttaccggc tagtcgagca actggtgaaa ccgatgggcg
    15961 tcgacgtgat ttgggtggac atgtgcgatc tcgaagctgt cgacgccgcc atgaacgatc
    16021 ggagcaaaat gctttggatc gaaacaccca ccaatcccct gcttcggatt ttcgatatca
    16081 cggcgctctc gaacctcgct catgcccggg gcgcatggtg cgtcgtcgac aacaccttcg
    16141 cgacaccgct cctgcaaaga cctctgcagc tcggtgcaga tttcgtcgta cattcgatgt
    16201 cgaagtatct caacgggcat agcgacgtcg tcgcgggcgc ggtcataagt caggccgctg
    16261 acttgtcgga ccgtttgcgc ttcatccagc gcgccggtgg agccgtgcct tcgccgttcg
    16321 attgctacct cgtcgcacgc gggatcaaga cgctcccgat acgcatgacg cgtcattgcg
    16381 aaagcgcgga agccctcgcc cagtggctaa agcaaagggc gggtgtggac gaggtgcact
    16441 atccgggcct cgaagcgcat ccccagcatg agctcgcggc tcgccagatg agcgccttcg
    16501 gtggaatgct ctcgttcgag gtctcgggtg gcctcgagcg cgcccgctcg gtgcttcagc
    16561 gggtccgtct tttttctctt gccgagagcc tgggcggggt cgagtcgctg atcggccagc
    16621 cggccctgat gagccacgcc tcgatgcccg aggacgttcg caagacacag ggaatccggg
    16681 atggcctgat tcgggtgtcc gtcggcctgg aggacgtcga agacctgcgc gaggacctcg
    16741 atcaggccct ttcgacgcca tagttgccgg gctttggcgc tctcgctaac ctgatcgcgt
    16801 ggggttaggc atgaacatga cgaggcgttc gatctgcgtg ctgctgccgt tgctggcgct
    16861 gtcctgcgcc gagggcactt ctccggtcac ggtggacgca cagtggaatc tcacctgtcc
    16921 agcggacagc gaggtcgggt gcggcgctct ggctcccgaa acctgcctcg gtccgatcgg
    16981 tcagcgtacg atcgcggggg aattccggga gatctcgtgc acgggcgatc caatcatcgc
    17041 cgtgtgcaac tccgtggagc gcgcggacgg tacgactttc atcgagctcg aagccaacgt
    17101 cggtaacgac ttcgcgttcg agctcacggc aatcgtcaac aggaacgacg actccgtagg
    17161 agccacgtgc gacgtaacca tcgtcgagga ccaactacct tacgacgttg gcgcttgcgg
    17221 tcgggaaccg ccgtcgatgg accagccttg tcagctcacg aacatcagca ccgacggcgc
    17281 tgaagtctcc ttcgaccttc aatgtgcttc gctcttgagc agcacgtcca gcctcggatt
    17341 cgacgtaggg gccgtcggtg gtggtcccac gacaatccga ttccagaact gcactggcag
    17401 tttctagagc ggcttctgag agtggtcctt gccgtcgaag cgaagagctg tcggcttctc
    17461 atcgacgacg gtgtgaacct cgaccgggcg tgtccaaacc cggctgagcg ccttgagaac
    17521 ctctttggcc caatcgagcc gcagatcggc gccctggtgc gtgtgttcga gtaggagctc
    17581 ggagcggttt ccgtggttgg cgtcgaccac cgaaatcagt ggatttcccg cgttggtgag
    17641 gctcgtgagg agctgggtct tcacattctc gaaagtgcgg ctttcgatct cccagcggtc
    17701 gtttcgctgg ttgtagccga acgagtacat cttctggtcg aacacaaagt cctcggtgag
    17761 gtactcgtcg atgaaagtga cgtcgttgta gagcgcgcgg acttcgaaga tcttttcgcg
    17821 cccaaggccg gtcctccggt cccaatgtcg ccgcagctcc caatcgtcgc aatcgtccca
    17881 ctccttgccg aactgccctt tgttccagcg atcctcgatg tgccgaaata gctcgatacc
    17941 gagcttgtag gggttgagct gccccggagc cgtcgcgagc acgctggcgc agcggtccgc
    18001 gtaatccacg atctcgctgg cgtcgcagat gttctccgtc atgagccgcg agtgccagta
    18061 gctcgcccaa ccttcgttca tgatcttggt ctgcatctgc ggcaagaagt aataagcttc
    18121 cgttctaacg accgcgaggc actctcgttc ccatcgctga agcggtgcgt tttccaacaa
    18181 gaagccgagc acgtcgcgcg cgggttccct ggggaaccgc tgcgcttgct ccgcctcttc
    18241 ttcgagcttc ttcctctgag attcgacgaa ttcctccggg ttgatgaacg actccatgta
    18301 gtcgcggtcg acgcgaagaa gctgaagctc tggcttctcc gggggctctt tctcctctga
    18361 ggtgcttggc gtgaggtcct tcggcaagaa cggtttctga gggtcgatca ggttctcgag
    18421 actcagacag gcatcgatga attgttcgac ggtttcgatg cctattcggt ttgcccactt
    18481 tcgaattatc gagccgttgt tcgccatggt gtcgatccat ttgcggacat cggcgctcgt
    18541 tcgcgaatct cgtccctggc tggtcatccg gaagcaaaaa ttgttcttga agaagtcgac
    18601 gtgcgcgtag acgtgcgcca tcacgagctt ctggtcgacc atgctgttgc cttcgagcag
    18661 gtacgcgatg gccgggttgt tgttgatcac gagctcgtag attttcgaga ggccatactc
    18721 gtggctcttg ctcagtcgct cgtactccat cccccaacgc cagtgcgggt agcgagtagg
    18781 aaaaccgcca tacgcagcga cttcattcat ctggtcgtag ctcagcatct cgaaaatcgt
    18841 cggaaagaag tcgagcccga cggttcgcgc gaacgcctcg atacgctgct gctcgtctcg
    18901 caggtagttt ggaagcgcgg tcttgagggc gaactgggcc acgcgctatt tgcctttccc
    18961 caggaacact cgaatcgact cgacgatggc gtcgcggtcg ttgatctcgc tcgtgatcag
    19021 ctgctcgtcc ccggagaagt gctctttgag gtccttgatg aactggccgg acccgtaagg
    19081 gctgtccacc tgaccgtagc aaaacacgtt cgatgcgggg agcaagagct tcttcaggat
    19141 gtccacgcag agcagcgtat cgtccacgct ccaattgtcg ccgtccgaga aatggaacgg
    19201 atagatgttc cactgttcgg agggatagtt ctgctcgatc atgtccgctg cgagtcggta
    19261 cgcggaagag atcatcgtgc cccccgattc ccgtgttcga aagaatgtgt cgcgatcgac
    19321 ttcacgtgcc gctgcatcgt ggatgatgaa tcgagtctcg atgtccttgt attgcttgcg
    19381 aagccaggta tcgatccaga atgattcgat gcgcacgatc tccttttgtt cgtcccccat
    19441 cgagcccgaa acgtccatca tgtagatgat caccgcgttg gcgacgggct cttcttcagt
    19501 cttccaggat ttgtatcgca tgtcgtcgcg ggtcggaatg atcaccggcc gcttaggatc
    19561 gtaggtgccg agcgcgatct gacggcggag cgcgcttcgg aacgtccgct tgaaatggcg
    19621 aagcgagttg ggcccgacgt tgcgaactcc gacgtatcga tccttcttgt cgacgatcgc
    19681 gctcttgccc ttgttctcga tgttggggag ctcgagctct tcaccgagga tgtctgcaag
    19741 ctcgtcgagt gtgagctcca cctcgagcgg gtggtcgccg gcttggtcgc ctgctttgcc
    19801 ctggccgtca ccttcctgag ggctgcccga caaagggtcg cccgggtctc cgtctccttg
    19861 agcgacaccg ccttgctgtt tgtcgccgaa gcgcagcttg gggatgtcga tgtgcgggat
    19921 cggaatcgag acgatgtcct ttccctgccg accgagcagc tcgcccttgg agatgtactt
    19981 gcggagattc tggcgaatcc gaccgcgaac gatgttgcgg aaccgggaat gatcctggtc
    20041 gatgcgcagc gacacccgtt actcctttac gtcgcccctt gcgaagatgc tggcgacgta
    20101 cgcgagcaca tcggtcgcag acgcctcgtt atacccatag tacttgatga gccgattctt
    20161 gatgatgtcg atcttgtctt gcgtctcttt gtcgatcacg ttcgaaacga agctcgacag
    20221 cttgatcgag tccttctggt cctcgaagag cttcatttcc aacgcgcgcc gcagccggtc
    20281 gttcgtttcg tatgtgaact tcttcccctc gagcgcgagg tgaccgatgt agttcatgat
    20341 ctcgcgccgg aagtcgtcct ttcgaggatc ggtgatgtcg atcttctctt cgatggaccg
    20401 catcagccgc tcgtcgggct cttcgtcctt gcccgtgtac ttgttcttta ccttttgttt
    20461 catcagatag gcgcggatgt tgtcgatgta gttcgcgcac agtcggccaa tcgcttcttc
    20521 gtcggcgctg atggctcgct gcacctcgtt cttgaccatt tcatcgtact cggccttcac
    20581 gacaccgatc agctcggcgt aacgtttgcc ctcttcatcg gcaacggtga gcgatgcaga
    20641 aaggcctttt tcgagctcgt tgagcaccat gaacggattg atgtagccct cgttaccgtc
    20701 acgaactagc gagttgctga tcttgtcttg cacgtagcga ggcgacatcc cttccagtcc
    20761 ctcgcggttc gcctccttgc ggagctcctt cacgttgtcc tgcgtatagc cggggagaat
    20821 ctttccatcg tagagcttca gcttctgaag aagagcgagc tgatgcttct tcggctcttc
    20881 gagtcgggtg agcactgccc acatcgccgc tacctcgagc gtgtgaggtg cgacgtgctt
    20941 gccgcgcagc cgttccgagc tgaagtcctt ttggtagatc ttgatctctt cgctcatctt
    21001 ggtgatgtac ggaatgtcga tcttgatcgt ccggtcgcga agcgcctcca tgaactcgtt
    21061 gttgattagc ttcttgtatt ccgcctcgtt cgtgtgaccg atgatcacct cgtcgatgtc
    21121 ggtttgagcg aacttcttgg gcttgatctt gtgttcctgc gtcgcgccaa gcaggtcgta
    21181 gaggaaggcg acatcgagct tcaagacctc cacgaactcc accacgccgc ggttggctat
    21241 gttgaattcg ccgtcgaagt tgaacgcgcg cgggtccgag tcggagccgt attccgcaat
    21301 tttgcgatag ttgatgtcgc cggtgagctc ggtcgaatcc tggttcttct cgtccttcgg
    21361 ctgaaacgta ccgataccga ctcggtcctg ctcgctcaaa atcaggcgct tcaccttgat
    21421 gtgattggcc atcactttgg accagtcgcc gttgtaacgt tcgagcaggc ttcggaagat
    21481 gaatcggcag gcggggttga gctcgccggg cacctgaatg cggcgccgat cgtcgcccag
    21541 ccgaagctct tgcaccgcac gctctcgcca ttcggttggg atcaggcgca gtggctcgtc
    21601 atgcatggga cacggaaaca catcatcgcc acctgcgagc cccgtctggt gcaagttcgt
    21661 ccactcgtac gtatagagag ccccctccgc cgtgcgtgag taacgctcga gccccttctt
    21721 gagcagacgc gcgatcgtgc tcttcgaaga gccgacggga ccatggagca agatgattcg
    21781 tttctcgggg ccgtagcctt gcgcagccgc gtgaagcacg tgcaccaaac gcatcagtgg
    21841 aatgtcgagg ccgaagatcg cgtccttgcc attctcgatc ggatctttga agaacggata
    21901 gcggatgagc cgctttttgt tgtcgatgta ctcttcttca ccgtacgaga cgaccatgtc
    21961 gtagaggcgc tgaaacgcgt tgcgcgtaac ctgcggtttc tccctaacga tctgaaggta
    22021 gtcttcgaag gagccttccc agtgtagatc ctggtagctt tcgtagtcct gcagggcagc
    22081 aatctgcttg accatttccc ccattgcgaa gtcctccttg gcgatcaggg tatcactaag
    22141 gcggatcctt gttaactatc gaagccaggc ggcgatgcat gtggcaacca cacatgcgaa
    22201 cacaaaccac cggagccccg cggcggggac cttgagcatc agacgcacgc cgattcgggc
    22261 gccgacgatc gatgcggcag ccaatacgat ggccggtgtc cacgacacca acccggccca
    22321 ggcaaatata ccgagggaga ccgtgccaaa ggcgagcgta accaccaact tgagcgcatt
    22381 cccggtgatc aggctgtgac gcagtgtccc aatgaggacg ccaagcaaga gaaaacccac
    22441 tcccgcctgg atgaaccctc cgtaaacacc agccagaaac aggcctgcat acgatttggg
    22501 ggcttggttc ggtctcagta tcggtccgtc atcgggaatg agtgtctgct ttcgaaaagc
    22561 gatcagcgcc gccatgacga tcattgtcac cagcatgatg ggcttcagaa cctcgcgtgg
    22621 aacccaggtg gcaagcagcg cgccgagcac cgctccgagc gtcgtgggga tcaggattgg
    22681 aacaaccgat ccggtttcca attttcccgc acggtgcatc gcgctggtgc ctgcgatgct
    22741 ttgcgtgacg actgcgaggc gatacgtgcc gttcgcaacg tccgcgggca acccgagcgc
    22801 catcagtgcc ggcagcacga tcattccgcc accgcccgcc atcacgttga ccaccgtcgc
    22861 gagtaccccg gctgccgcga ggatcgccag gctctggtaa ctgagctcct cgaccatgaa
    22921 agttccgctg tcccactata gccggttttc gtttctaggt acttcggatg ccaaagcgtg
    22981 ctagcgtccg ctgacgtgaa gaagacctac gtcttggata ccaatgtggt tcttcacgac
    23041 ccgttagcgg tctacaaatt cgaggacaac gcggtcgtcc tacccatctt cgtgatcgaa
    23101 gaggtggacc agttcaagaa agagctcagc gagctcggac agaacgcccg acacctatca
    23161 cggattctcg acgacctccg aaatcagaac ggtcacactc tcgaccaagg ggtgccgctc
    23221 gccagcaccg ggggcgagct tcgagtagca gtgcctccca gaatgcccaa gcgggcgagt
    23281 ggcccaggcg agatggatcg cttcatcttg gagatggcgc tcgagcttcg cgatgagaat
    23341 ccggaacagc ccctggtgtt cgtcaccatg gatacgaacc tccgaattcg cgccgacgcg
    23401 cttgggctca aggccgaaac ctacgagggc gggcgtgttg cgatcgacga cctctattcg
    23461 ggcgtgatca cggtcgacgg ggatcgggag ttggtcgata atctggctcg gcgcgagccc
    23521 gtcgaggcga gcgctctcgg cgtcgagctc tacccgcatg ccggcatcgt tcttcgagag
    23581 cacgaccggc ctcggcatac ggcgctcggg cgcttcgatc cgaagagcgg gaacatcgtt
    23641 ccgctccgag tgggaaggga aggcgtctgg cgggttcgtc ctcgcaacct cgagcagtat
    23701 ttcgcgctcg acatgcttct ctctcccgac atccacttgc tgaccttggt aggcaaggct
    23761 ggcaccggaa agaccttgct cgccattgca gcaggcctgc aatctgtggt ccaggagggc
    23821 acttacagtc gaattctggt ttctagaccg attttcccga tgggccgtga cgtgggttat
    23881 ctgccgggta cagtggaaga gaagttgaac ccttggatgc aacctatctt cgacaatctc
    23941 gagtacattt tcagcagtgg ccgtggccgg ctgtcggagc accgaaatta cggcgacctg
    24001 atctcatcgg gcgtcatcca agtggaacct ctcacgtaca tccgtggtcg ctcgcttccg
    24061 aatcagttct tggtcgtcga cgaagcgcag aatctcacgc ctcatcaagt caagaccgtc
    24121 atcacccggt gcggtgccga caccaagatc gtcctcaccg gcgatcctta ccaaatcgac
    24181 aacccctatg tggacgctgc gagcaacgga ctgaccgtcg tcgcccagcg cttccagaac
    24241 gagagcatcg gggcccacgt cacactctcg aaaggtgagc gcagtgagct cgcagagcgc
    24301 gcgacgcaac ttctttgagc gtcctcgcgt tcgccttcgg gccgagctcg acgtcgcgct
    24361 tcgccgcctg cacggtaagg cgcgcttgct cgtcggttcg accacgcaca gcgatcctgc
    24421 ggagaggcac aggttgtgcc aatgcgtggc caggggagag cacgcgaaac ctcgctggga
    24481 atggaggcct gtagcgatag agcgcagcgt ttggcgtgag ctcgcgcgcg cacgagaact
    24541 tgccgaagct tccgaagcag cagcgcttta tcttccacgc ctcgaagagc tcgaaatcga
    24601 gctgctgctt ctggagtcgc tcggccgcag caagcaagtg cgtccgatgg cggcgcggct
    24661 cttcggaacc ggcagcgagc ggctattccc ggattcgaac cacagcatcc tcgacgccgc
    24721 tcacgagatc ctctcgaacg cgtcgctcga acaagaagag aagacgattc cggctgcatc
    24781 cgtcgagggg gtcaatctgc gcgatttgat gctcgcctat ggcaagcacg tgggtttgca
    24841 catagcggtt cgcatcgatc cgggtctaat cgccaacgcc gccgtcggcg agcgcaccgt
    24901 gttcatcgcc gacaggtgct ttggcgccca cgaagcacag cgccttgcga cacacgaggt
    24961 gtatgggcat ttggtgtccg ggttcaacgg caggacgcaa acgctgggga tcttctccat
    25021 cggcacggcc gggtcgtacg gcgaccagga gggcgttgca atttatctcg aggagctcgc
    25081 agggctcctc gactcctctc ggcagcggac tctggctggc cgtctgctgg ccactcatgc
    25141 aatgcacgcg ggcgtatctt tcggtgacgc ggcgagggtc ctcgtaagtg agcacgattt
    25201 ctcgccggag gcagcggtga ccctctgcga gcgagcattc cgcgcagggg gtgtagcgcg
    25261 cgacgcggtc tatctcactg gctggttgcg agtgcgccgc gccgtcgctc gaggcgaaac
    25321 caatctcgcg gagctccagc tcggcaaggt ttcactgagc gccttgccgg agctgcgccg
    25381 tctagctcgc gacggtctcc tgactcagcc ggtctatttg tcgaatttgg ccaagagcct
    25441 tggaagcacg ggtgcgggca cgaggctcgc cacgttgcct ccgagcctag cgacctcttt
    25501 gacgaggttg gacgctacgt agaagtaagc gtcgttcgcc atgatgaaca cagtctcgat
    25561 ctcggggctg agatgtcggt tcatgtttgc catttgaagc tcgtactcga aatccgcgac
    25621 ggctcgaaga ccgcggagga tgatgcgggc gttcctccgt cgcgcgtaac cgaccaagag
    25681 gccatcgaag ttgtccacgt cgattcgccc gggttcgacc cccatgtcgt ccgctgattc
    25741 acggatcatc tccattcgtt ctgccacgct gaagaggggt ttcttgttgt cgttgttgag
    25801 tacggcgacg atgatctttt cgaacgccac gagcccactc ttcagaatgg cagtgtggcc
    25861 cttcgtgatc ggatcgaagg aacctggata aatcgcgatg cctctcactg cggacctttc
    25921 ccgggtgcgt agacgcccaa gctgataccg gtctgaccat agcggtagtc tgcttcaggc
    25981 gcgagcccct ttggccatct ccagtcgtgc gtggctgagt gctcgaccac cagcaaagcc
    26041 cctggcgcca ggcgccccga ggctgtgaga gcattcagca gcgctgatac cgagtcggtg
    26101 tccgagtagg gtgcatctgc aaacagcaga gtgaagctcg actccgttgc gggaagcttg
    26161 cggattacgc cggacggatc agcgagcaga tcgaggcgaa tcgttcgaat ttttcctgcc
    26221 aaccctagct cttttgcgct ctggttgatc tgtcgaatcg cgccagggtc ctgctcgacc
    26281 agcactgagt gcttggctcc acgcgagagt gcctcgaagc tgagggcgcc cgtgccggcg
    26341 aagaggtcga gcacctgcgc gtcgccaaac gcattgcgcg cttccagagc cgaaccgagt
    26401 gcttcccgga cccgatccga agtcggtcgc gtgccgcggc cgagcggtgc actgaaccgt
    26461 cggccagaga gccgtcctcc gactatgcgc atgacctcag cacctggtgc ccagacccgc
    26521 caaagccgca tccatgacca aaaacactca tcgcaatgcc tcgtgccgaa ttgctttgga
    26581 gcacagcgag caacgctctt caactccgaa atgcgttgcg aatcactttg gaaaacgacc
    26641 gcgaagactg aagctcggag ccgaatgcgc gtctgaaggg ggaattgtac tacccactgc
    26701 cagatgctac gcagagcgag cgttcaggaa agcctgccga catgcggaag atttgccttc
    26761 gttgcggttc gaagttcggg agccgcgccg aatactgccc gcacgacggc agtcttctcg
    26821 tcaccgatga tgagatcccg gacccattga tcggaacggt cttgctcgaa cagttccgga
    26881 tcgacgagca gatcggtacc ggcgggatgg ggacggtcta tcgcgcaaat cagaccacgg
    26941 tcgggcgcga cgtggccatc aaggtcttga gggccgagct ggcgcgtgac gagcaggcag
    27001 tgtttcgatt cgagcgtgaa gctcgggtcg cgatctccct cgaccacccg aacctcgtcc
    27061 gagtgtttct ctccggtcgg ctgaaggatg gacgcctcta catcgtgatg gagcttctcg
    27121 aaggcagatc gctcgccgac gagctcgatc aggaaggggt gccgtctctc gaacgcgtgc
    27181 tgatcatgat catgaagctg tgcgccggtc taggcgctgt acacgcggcc ggtatcgtcc
    27241 accgggacat caaaccggag aacgtctact tggtgcggcg ggggagggac aacgatttcg
    27301 tgaagctcgt ggacttcggc atcgctcgca tcctcgcagg ggaaggcatt gggccgacca
    27361 cgacgcaaag cggccgcgtc ttcggaacgg cgacctacat ttctcccgag gctgcaaccg
    27421 gtgaggagac cgaccagcgg agcgacatct attcgatggg ggtgctctcg taccaactct
    27481 tgaccggaga gcttccgttc gagggcaaaa ccgcaggcgc ggtcctgatg cagcatgtgc
    27541 atcaggaacc gccgctgctc cagaccgtgg gccgcggcgt ggaggttccc gatgacctcg
    27601 caggtgtcgt catgcgcgcg ctcagcaaag accccgatgc gcgccaacaa tcgcttgccg
    27661 agttcctgaa tgcgctttcg caggcggcgg gtgaggccgg gcttctcaac gacgcgcgcg
    27721 ctcttctgtt tggaacgata tggggtgagg atttggctgc cccgggctca tatctggccg
    27781 agctgctcgg ttcgagtgcc cctccgggct cgccttccgc cctggccgaa acgcaacctg
    27841 cgcaattggg tcgtgaccca aaggaagagg ccctgctctc gtctgcgcca aaaccgttcg
    27901 gcactacggc gtacgacctg gtggctcccg tgcgtaaggc caacggtcga aagcaccgct
    27961 ggctctgggc ttcgctcggg ctgctctgcc tgatcatgat cggcctggtc gtgggagcct
    28021 ggtggttgca gcgcccgccc cgggccacca tcgatgagac aatcgccgag cccgtgctgg
    28081 ctccgccggc ggtcgagatc ggtgtctcga acgagaccga acagcagaag ccacccgtcg
    28141 agaaagcggt tccggacgaa cccgttttgg aggcgaaggt ggagccgaat ccgaagaaga
    28201 agaaaagaac gacgacgaag cgaacgggag tggaagcagg gacagcggca gcggcagcgg
    28261 cagcggcagc tacagagata gggacagcgg cagagacagg gacagggaca gagacaggga
    28321 cagggacagg gacagggaca gagacagcga ctggggaggg tgctgcgccc gatgatggga
    28381 tcgattggag cgttcctgat tttcctcagg agccgccagc agctgcgaac gcggcgccga
    28441 gcgaac
//
LOCUS       DASRZF010000311        14427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_583653, whole genome shotgun sequence.
ACCESSION   DASRZF010000311 DASRZF010000000
VERSION     DASRZF010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 14427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14427
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583653"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..1707
                     /locus_tag="VFG22_13715"
     CDS             175..1707
                     /locus_tag="VFG22_13715"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET6417348.1"
                     /translation="MPRIPAFLRSTRVCLILSLGLSSCATDTTAPVGEWPTASPEQQG
                     FDSAEIAAVVEEIDSKGLPVDSLQVVRNGVLILDAYFYPYLGDRPHDLASVTKSVTST
                     LVGIAVDQGLLTLDQNLIASFPELVPVPPTDGKEDIELSHLLTMTSGLDCGRLPGEQE
                     LNAMLGSDDFVEYALGIPMAAPPGGEFAYCSPGTHLMSAMLADAADSNTLEFAQENLF
                     GPLGITELVWPTDPQGVNRGWGDLQLYPLDLAKVGQLFLDGGEWNGERIVSKSWVDEA
                     TRAHVLADTDGTGYGYQWWRPAAFEGVYQAIGRGGQSMIVWPDKNVVAVFTGRGVDTT
                     GEVALALAAALKGDEPLPPNREGNARLNTAIQEASEPPPVQPIAPLPPMAEEVSGKVY
                     RLAPNQFDVKCITLRFDSPSEVWFDLSLGTGEFELPVGMDGVPRFSDTGPTGIPVGVL
                     GEWTEPSLFSMQYDEVAGPNHLRIRGDFGDSAETVELEFTDPGEYFPAQTVQGSAAAS
                     CN"
     gene            1786..2370
                     /locus_tag="VFG22_13720"
     CDS             1786..2370
                     /locus_tag="VFG22_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6417349.1"
                     /translation="MGKGEDTKAAIVDQALNLVSTVGLEKLTIGALASATGMSKSGLF
                     AHFDSKEQLQLQVLDEARKRFVDAVIAPALKKPRGEARIRALFENTMQKWETALPGGC
                     VFSAVAAELDDQPGPARDYLVEVQKDYQDVVKRAAQVAIDVGEFRKNLDLGQFAFEFG
                     SITAAYHHFGRLLHDSRAEQHAYSAFEALLSRSH"
     gene            2577..3278
                     /locus_tag="VFG22_13725"
     CDS             2577..3278
                     /locus_tag="VFG22_13725"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6417350.1"
                     /translation="MLRGATEMTLAGMNAWSWGEGPTILLVHGWNGRATQLGAFVSPL
                     MTRGYRVVAFDAFGHGESPGNYLSLPELATCIRQVTDELGGVYGIVAHSLGGAATTFA
                     LAKGLEIERAVFVSPPADPREFLAAFSAALGVSDEVRTLTMRGLEERVGTAMEGMRAT
                     ALAPSMRTPLLVIHDRDDKEVPARVGQSIVEAWPGAELILTRGLGHQRILRDATVTHF
                     AVRFIDAPKHRQAAA"
     gene            3299..3493
                     /locus_tag="VFG22_13730"
     CDS             3299..3493
                     /locus_tag="VFG22_13730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417351.1"
                     /translation="MNTEIRDPQFAISINEIANAIRWPDPSFALSPIEAAIDVLVAFY
                     DAFARPDRVILEHALKGIAF"
     gene            complement(3531..3902)
                     /locus_tag="VFG22_13735"
     CDS             complement(3531..3902)
                     /locus_tag="VFG22_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417352.1"
                     /translation="MRFLATAASLLLALLLTTGCGDDRPPGTAGNESTLVGGPCEDNL
                     ECDEGLCQKGALFPDGICTISCPSSNLCPSGSSCAETTLGWLCLVDCETNSDCREGYA
                     CESMTEAGTSGSSTVLVCIAP"
     gene            complement(4115..5005)
                     /locus_tag="VFG22_13740"
     CDS             complement(4115..5005)
                     /locus_tag="VFG22_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6417353.1"
                     /translation="MVASKIREERVAFEVESVDVVGVLRVPVGAHASAALVFAGPMTS
                     VKEQATGNYAMALAARGFVTLAFDHRHFGESGGEPRQYEHPGRKVEDFRAAFGFVASR
                     PEVDPNRIGAVGVCAGAGYLAPAVAGDDRCKAWGTVAGFFHDALIQRRWFGDAYEPRL
                     EEAIDAREHYEDTREALAIPAVGDGDVAMPLHEAFEYYGTERGVVPNYVNQFAVMSGE
                     HTLPWDAQSSAYDITVPTIMVHSERAAAPPLARKFFKSLAGLKDEVWLDTENHIDFYD
                     RSEQIDAAANELSRHFRSHL"
     gene            complement(5056..6570)
                     /locus_tag="VFG22_13745"
     CDS             complement(5056..6570)
                     /locus_tag="VFG22_13745"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET6417354.1"
                     /translation="MESNLHTFVVSDMHLTEAEEPHPDRPLWMAYKRREFFIDQDFAA
                     FLDHIEEKTNGPIELLLNGDIFDFDSVTALPNENRHIEWLERSRGLGSEEWKSQFKMQ
                     RIIEDHPLWFERLGRFIARGHRAVFIIGNHDVELYWPSVQRMICDALGAPLPSNINEE
                     EDDDPIVFCNWFYISGGDTYVSHGHQYDPNCVVRDPIDPLIEVRGQPRVRIPFGDLAA
                     RYMLNGMGYFNPHQSENYIMGAVAYLRFFFRYMLRTQPLLIWTWLWGAYATLWVSLRT
                     HWLPAMRDPMLVDDKVRSIATKSQATPSMVRKLNALHVPSATNNPFRIARELWLDRAF
                     FLLAALFLSWQVVLLINIAWPISPLWVFVPSLIFMLPYLAYASSVRPTVFETPLLTEQ
                     LADLIFKITGARRVVFGHTHQPQCEQVGPVTLYNGGFWSLAFADPECTIRLGEQTFVW
                     IRPSEEGFERVAELCEWKAGEKTPMRSLCTDGSEALKSHSHPALQDQGRGLSAS"
     gene            complement(6654..7298)
                     /locus_tag="VFG22_13750"
     CDS             complement(6654..7298)
                     /locus_tag="VFG22_13750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417355.1"
                     /translation="MSKAMDAFYRFLDEPIFPWARVVLALLTIFVGLSFTAPMWYINM
                     KAPQYPEGLDLYIYSYKVEGGNDGNDVQEINVLNHYIGMAPLDRAALSDLDWIPFLFA
                     AMIIFALRTAVIGKVSSMIDLLVMTTYAGLFGFGRLYYKLYTFGHNLDPRAPVTVDPF
                     TPVMLGKKQIANFLTEAGPGLGTLYVTIFATGLTLLLLAHLFIGRKRHLASQGK"
     gene            complement(7325..8167)
                     /locus_tag="VFG22_13755"
     CDS             complement(7325..8167)
                     /locus_tag="VFG22_13755"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET6417356.1"
                     /translation="MNRQLRSYWAVAQLDLAEVLRSRWLLFCVVVYAVLAGVFLLVGM
                     RESTVFGFTGTDRVLVSYVHALLVLLPLMSLIATGQVVNKARDDGTLEILFSQPISRG
                     AFFSAISSVRFLVLLLPLALLMAAVGLFGRFAFGQQIAWSFIGHCLLLCSVLVWSFCG
                     LGLWVSTHVQNSARATMYLLLIWVAGIALMDFALAGMMLQWRMEPRVVFALAALNPVQ
                     SIRMALLSSADPELSVLGPVGFYLANRIGSWGLYALGVGWPTVLGTLAWLSGLRTLQR
                     GDVV"
     gene            complement(8164..8619)
                     /locus_tag="VFG22_13760"
     CDS             complement(8164..8619)
                     /locus_tag="VFG22_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417357.1"
                     /translation="MASKLMKALLLALALVVVVVGFMRFNEVTPPEGPVPVVWDSEVC
                     QHCKMHVGDPRFAAQLQTSDGGVMNFDDPGCLFDYLQSHPVIIHALYFRNHEGEGWLS
                     ESEVGFLPVEDSPMGYGIRAVPGTAPEAQNIDWAKAQVMNRPQRWDGGS"
     gene            complement(8609..9496)
                     /locus_tag="VFG22_13765"
     CDS             complement(8609..9496)
                     /locus_tag="VFG22_13765"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6417358.1"
                     /translation="MRVEGKAVRKRFGKVQALRGIDFTIPSGSKVGLIGPNASGKSTL
                     IRIILGLLNCEGELLLDGEKKRQMELADRIAYVPQIAPKFGASVGEVVKAITRVREMS
                     PDAVLACGAEVGIDLGAVGRQAFCNLSGGAKQKTLIALALASQASLYVLDEPTASLDT
                     QSRRDLFHLLSERTKDATLILCSHRLEEIRTLVDQVMVLEEGTLAYFGPTEDYLDRMT
                     LSTIDVQMKNGAGSEDLESLGFQPGVAGWWTRTATRAEKLELVAQLSDGLEGRIMNLL
                     VRDADAVQLDRQGGSDDGQ"
     gene            complement(9486..10844)
                     /gene="nosD"
                     /locus_tag="VFG22_13770"
     CDS             complement(9486..10844)
                     /gene="nosD"
                     /locus_tag="VFG22_13770"
                     /inference="COORDINATES: protein motif:HMM:TIGR04247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071941 - nitrogen cycle metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HET6417359.1"
                     /translation="MSRSAARVGLELNARNHACVCFLVLMTVAACATEAAPPLGKEAA
                     LAPPPRPNDCEVVAAGANLQAVVDGAEEGAALCLESGSYSGPLSVAKSVEIWGPRDAV
                     VRSAGEGTTIEVQADGSSLSGFTVDGSGGRFDTLDAAVHVVADDVRVEGLRVQNAAFG
                     LLIEKSNRAAILNNVVQGPDEGPLGLRGDGIRLWETRHSEVRDNQVIGCRDMVVWYSS
                     NNRLVRNTVVRSRYGTHFMYSHENHVEGNRYLDNVVGIFIMYSRNLTLRENLLARSSG
                     PGGMGLGIKESGNLTVADNAFIANTKGVYMDTAPLEPDDFNTFENNAFFHSDVAVLMH
                     SSETRNAFRGNQFVSNHSQLQVEGGGNALEVEWRGNSFDDYAGYDMDRDGFGDIPYEL
                     RRLSSQLESTYPQLQFFRGAITLELLDAISSLFPMIEPETTLIDPRPRMSAVDWKILD
                     AR"
     gene            complement(10841..12811)
                     /gene="nosZ"
                     /locus_tag="VFG22_13775"
     CDS             complement(10841..12811)
                     /gene="nosZ"
                     /locus_tag="VFG22_13775"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR04246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HET6417360.1"
                     /translation="MRFKRMGALCASGVAALCAAVVSIGCGPQQAAQVSQAPASSTAD
                     LMKQRGLSEADVSAALQTYTPTGKKDAYLTFASGGHGGNLIVIGVPSMRILKYIGVFT
                     PEPWQGYGYGDQSGEVLAGGSRFGKEMTWGDMHHPALSETNGEYDGKFIFVNDKANAR
                     IAVIDLHDFVTREIVTSTLIQSDHGATFVTPNTEYVVESSQYPAPLGGGYEPIENFQD
                     KYRGAVIFWKFDREKGKIDRANSFAMELPPYMQDLADAGKLDSEGWVFINSFNTEMAW
                     GGTMEGNPPMESGASQNDMDYLHIIDLKKAESVYKAGKTTKIAGMDVISLETTNAEGL
                     LHLVGEPKSPHGVDVTPDGKELVVSGKLDTHATVYSFEKLKGLIEAKKYDGKDQYGVP
                     ILPFNDSIRGQVEIGLGPLHTQYDDKGNAYTSVFIESTVAKWSLKDLKVLEKVKVHYN
                     VGHIVAAEGDTVSPDGGYLIAMNKWALDRFSKVGPLLPQNFQLININSEPMQLIYDMP
                     LPLGEPHYAQMIKADKLDPVDVYKPSGYDVVTQAHSPNAVKAKEERIEREGKDVHVYM
                     TSVRSHFTPDIIRVKKGDNVHLHITNVEQAHDATHGFTIGSYNVHSSLEPGKHVDITF
                     LADKEGTFPFYCTEFCSALHLEMAGYLLVEPS"
     gene            complement(12964..13389)
                     /locus_tag="VFG22_13780"
     CDS             complement(12964..13389)
                     /locus_tag="VFG22_13780"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET6417361.1"
                     /translation="MRAKWIGLLIGLIVGISGCTPKDAAPAEQKAAEPAPAPAPAAEP
                     ASDPVAEAAEIFQSRCTVCHGESGMGDGPGSAALEPKPRDFTSAEWQASVTDEHLQKI
                     IVYGGSAVGKSPSMPANPDLDAKPEVVAELVKYVRDLAK"
     gene            13591..14154
                     /locus_tag="VFG22_13785"
     CDS             13591..14154
                     /locus_tag="VFG22_13785"
                     /inference="COORDINATES: protein motif:HMM:NF014522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HET6417362.1"
                     /translation="MRGFVKITVIGGLLCGLLVAGCKKQEAAPAEPTPSEVAPAQEVS
                     NIPPLNENNIVSIAINSKDHTTLVAAIKAADYVTSVAASGPLTVFAPTNEAFAKLPEG
                     TVENLVKPENVATLRNILQYHVTTSALQLDWFKDGQTVSMANGKRTTMNVKDGKVTIN
                     GANILATIPAENGIVYVIDSVLLPPEK"
     gene            14164..>14427
                     /locus_tag="VFG22_13790"
     CDS             14164..>14427
                     /locus_tag="VFG22_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417363.1"
                     /translation="MTLDSAWSLVRGLRVVAALLSMTPLFACTNGGVSDVPLDAGMDA
                     GPLEPVEDGTYFVHDDDERVVILRGMNIMSSAKGHPERLPNLEE"
BASE COUNT         2879 a         4425 c         4390 g         2733 t
ORIGIN      
        1 cctgctcgcc gaccaccgtc atgctagaac tgacgcgcgg ccgtgccaaa tctttagacg
       61 agacgcccat ttttgcctaa tgcttgcgct cgcgagtgct gcttcgtcga atggacctgg
      121 ccgctgaacg caggtaagct gccgtacagc accgtggtgt tggagctgct tctcatgcca
      181 agaatcccag catttctgcg ctcaactcgc gtatgcctga tcctttctct cggcctctcg
      241 agttgtgcga cggacaccac agcgccggtt ggcgaatggc caacggcaag cccggagcag
      301 caaggtttcg actccgcaga gatcgcggcg gtcgtcgagg agatcgactc caaaggactt
      361 ccggtcgata gcttgcaggt cgtgcgcaat ggcgtgctga ttctcgacgc ttacttctat
      421 ccgtatctcg gcgaccgtcc tcatgacctg gcctcggtca ccaagagtgt gacatcgacg
      481 ttggtcggca tcgccgtcga ccagggtctg ctgacgctcg accagaatct aatcgcctcg
      541 tttccggaat tggtgcccgt gccaccgacg gatggcaaag aggacatcga gctcagtcat
      601 ctattgacga tgacctccgg tttggactgc gggcggttgc caggcgagca ggagctcaac
      661 gcgatgctgg gcagcgacga cttcgtcgag tacgcgctcg ggattcccat ggccgcgcca
      721 ccaggaggag agttcgctta ctgcagcccg ggcacacatc tgatgtctgc gatgctcgcg
      781 gatgccgctg attcgaacac actcgaattt gctcaggaga acctattcgg ccctctggga
      841 attacagagc tggtatggcc gacggacccg cagggcgtga accgtggctg gggcgatctg
      901 caactctacc cgctcgactt ggcaaaggtg ggtcagctgt tcttggacgg cggcgagtgg
      961 aacggcgagc gcatcgtgtc caagagttgg gtcgacgaag cgacccgggc ccacgtgctc
     1021 gcggacaccg acggcaccgg ctacggctat cagtggtggc ggccagccgc attcgaaggc
     1081 gtgtaccagg cgatcggtcg cggaggacag agcatgatcg tctggccgga caagaacgtc
     1141 gtcgcggtct tcaccggacg cggggtcgac acaacaggtg aggtggcact ggccttggcc
     1201 gcggcgctga agggcgacga gccccttccc cccaaccgcg agggcaatgc ccgactgaac
     1261 acggcgattc aagaagcctc cgaaccgccg ccggttcagc cgatcgctcc gctaccgccg
     1321 atggccgaag aggtttcggg gaaggtgtac cgcctcgcgc cgaaccagtt cgacgtgaaa
     1381 tgcatcacgc tccgtttcga ctccccttcg gaggtctggt tcgacctcag cctcggcact
     1441 ggtgagttcg agcttcccgt gggaatggac ggtgtgccgc gattctccga caccggaccc
     1501 acggggatcc ctgtgggggt cctcggtgaa tggaccgagc ccagcctgtt ctcgatgcag
     1561 tacgacgagg ttgctggacc caatcacctt cgaattcgag gtgatttcgg tgacagcgcg
     1621 gagacggtcg agctcgagtt caccgacccg ggagagtact tccctgcgca gacggtacag
     1681 ggctcggcgg ccgcgagctg caactgaagc gcttgattta actggctaat tacgattctc
     1741 cgaatctttt gttgacgaaa cgaacggtcg tactattatc gtcacatggg caaaggtgag
     1801 gacactaaag cagccatcgt cgaccaggcg ctcaatctcg tgagcacggt tggcctggaa
     1861 aagctcacca tcggagcgct ggcgagcgcc acgggcatgt ccaagagcgg cctcttcgct
     1921 cacttcgatt cgaaggaaca actccagcta caggtcctcg atgaggcgcg gaagcgcttc
     1981 gtcgacgcgg tgatcgcgcc agccctgaag aagcctcgag gggaggcacg cattcgggcc
     2041 ttgttcgaga acacgatgca gaagtgggaa acggcgctcc ccgggggctg cgtgtttagc
     2101 gccgttgccg ccgagctcga cgaccagcct gggccggcgc gcgactatct cgtcgaggtt
     2161 cagaaggact accaggatgt cgtgaagcgc gccgcgcagg tcgccatcga tgtcggcgag
     2221 ttccggaaga acctggacct cgggcagttc gccttcgagt tcggatcaat caccgcagcc
     2281 tatcaccact ttggccggct cctgcacgac tccagagccg agcagcatgc ctatagcgcg
     2341 ttcgaagcgc tgctttcgcg ttcgcactga ggagggagac gatgaccact gtcactgccg
     2401 atacaacaag acgacccatt aaaagcacga tcgttcgttc taataacttc ccgccgccat
     2461 ggatgagatc gacattttcc gcggcgagtc agctcgcacc cgggctcacg gcaagagtcg
     2521 ccgcgaggct gtttcgaagg acacggaggc gaaacgcccg acccggggag cgcgaggtgc
     2581 tgcggggggc gaccgagatg acgctcgcag gcatgaacgc ctggtcctgg ggcgaaggcc
     2641 ccacgattct cctcgtgcac ggttggaacg gccgggccac gcagctcggc gccttcgttt
     2701 caccgctgat gactcgtggc taccgagtgg tggcgttcga tgccttcggc cacggtgagt
     2761 cgccggggaa ctacctctcg ctccccgagc tcgcaacctg cattcgtcaa gtcaccgatg
     2821 agctcggggg cgtgtacggc atcgtcgccc actcgctcgg tggagcggca actacgttcg
     2881 ccttggccaa aggtctggag atcgagcgtg cggtcttcgt ttcgccgccg gccgacccca
     2941 gagagttcct cgccgccttc agcgctgcac tgggtgtcag cgacgaagtt cggacgctta
     3001 cgatgcgggg gctcgaagag cgcgttggaa ccgcgatgga gggcatgcgg gcgactgcgt
     3061 tggcgccgtc gatgcgaacg ccgcttctcg tcatccacga ccgagacgac aaagaagtac
     3121 cggcgcgcgt tggtcagagc attgtcgaag cctggcccgg agccgagctc atcctcactc
     3181 ggggcctggg acaccagcga attcttcgcg atgcgaccgt aactcatttc gcggttcgtt
     3241 tcatcgatgc accaaagcat cggcaagcgg ccgcctgaat caagaaaggc agagaaccat
     3301 gaataccgaa atcagagacc ctcaattcgc aatctccatc aatgaaatcg caaacgcgat
     3361 ccgatggccg gatccctcat tcgcgctgag tcctatcgaa gccgcgatcg acgtgctggt
     3421 cgcgttctat gacgcgttcg cccggcccga ccgcgtgatt ctcgagcacg ctctcaaggg
     3481 gatcgcgttt tgaatcgaca cgagccgatg acccgaaacc aggaagccgc tcagggggcg
     3541 atgcagacca agacagtcga gctgccgctc gtccccgctt ccgtcatcga ttcgcaggca
     3601 tagccctcac ggcagtccga atttgtctcg caatcgacca ggcaaagcca tccgagcgtg
     3661 gtttccgcac aggacgagcc agaagggcag aggttggagc tcgggcaaga gatcgtacag
     3721 atgccgtcgg gaaagagcgc gcccttctga cagagccctt cgtcacattc gaggttgtcc
     3781 tcgcaaggcc ccccgacgag cgtactctcg tttccggcag tgccgggcgg acggtcatcg
     3841 ccgcagcccg ttgtgaggag cagggcaagc aacaaagacg cagccgtggc taggaacctc
     3901 atgtcggccg gacgatagat caaggagagc ggccgaacca gcgccgccgg gccctgccat
     3961 gtaacattcc tgtcaccttt ttggcggctc cgctcaacga cgctcttcga gctcggagat
     4021 ccgggtttcg atgcgaacgt cctcttcgaa cggctcgcga cctgctaaac cgaattcggg
     4081 caacgctggc tcttaccggc acctacccct ttgtctacaa gtgagaccgg aagtgccggc
     4141 tgagctcgtt cgccgctgca tcgatttgtt ccgaccgatc gtagaaatcg atgtggttct
     4201 cggtgtcgag ccaaacctcg tccttgagac cagcgagcga cttgaagaac tttctcgcga
     4261 gcggaggggc cgcggcgcgt tctgagtgaa ccatgatggt gggcaccgtg atgtcgtaag
     4321 ccgagctctg cgcgtcccat ggaagcgtgt gctcaccgga catcaccgcg aattggttca
     4381 cgtagttggg aaccacaccg cgttcagtac cgtagtactc gaacgcctcg tgaagtggca
     4441 tggcaacgtc accatctccc acggcaggta tcgccagagc ctcgcgcgtg tcctcgtagt
     4501 gctcgcgcgc atcgattgcc tcctcgagcc gcggttcgta cgcgtcgccg aaccagcgtc
     4561 gctgaatcaa cgcgtcatga aagaaaccgg ccacggtgcc ccatgccttg caccggtcgt
     4621 cacccgcgac ggccggtgca agatagcccg cgccagcgca gacacccacg gcaccgattc
     4681 gattcggatc gacctccgga cggctcgcaa cgaatccaaa cgcggcgcgg aagtcctcga
     4741 ccttgcggcc cgggtgctcg tactgtcgcg gctcgcctcc gctctcgccg aaatgccggt
     4801 ggtcgaatgc aagcgtgacg aatccacgcg cggccaacgc cattgcataa ttacccgtgg
     4861 cttgctcctt gacgctggtc atcgggccag cgaagaccag agcggccgag gcatgcgcac
     4921 ctaccggcac gcgcaagact ccgactacat cgacggactc gacctcgaat gcaactcgct
     4981 cttctcgaat cttggaggcc accatgaggt ctcaacgtcg gtctacatac gttcccgtca
     5041 agtgtcgaca aggaatcaag atgctgagag cccccgtccc tgatcttgga gggcaggatg
     5101 cgagtgggac ttcagggcct cggacccgtc ggtgcaaagg gagcgcatgg gtgtcttctc
     5161 gcccgccttc cactcgcaga gctcggcgac tcgctcgaaa ccctcttcgc tcgggcgaat
     5221 ccaaacgaat gtttgctccc cgaggcggat ggtgcactcc gggtcggcaa acgcgagcga
     5281 ccagaagcca ccgttgtaga gggtaacggg acctacttgc tcgcactgcg gctggtgtgt
     5341 gtgcccaaaa accacacgac gcgcgccagt gatcttgaag atcaggtcgg cgagctgctc
     5401 ggtgagaagc ggtgtctcga acaccgtcgg tcggaccgag gacgcgtacg cgagatacgg
     5461 aagcatgaag atcaaactcg gaacgaagac ccaaagaggg ctgatcggcc aggcgatgtt
     5521 gatgagcagc acgacctgcc aagagaggaa tagcgcggcc agcaagaaga aagcacggtc
     5581 gagccagagc tcccgcgcga tccggaaggg gttgttcgtg gccgacggaa cgtgaagtgc
     5641 gttgagcttt cgaaccatcg aaggcgtggc ctgcgacttc gtcgcaatcg accgaacctt
     5701 gtcgtcgacc agcatgggat cgcgcatcgc cgggagccag tgcgtccgca aagagaccca
     5761 cagtgtcgcg tatgcccccc agagccaggt ccaaatcagc agcggttgcg ttcggagcat
     5821 gtatcggaag aagaaccgga ggtaagcgac ggcacccatg atgtagtttt cggactggtg
     5881 ggggttgaag taccccatgc cgttcagcat gtaacgcgcc gccaagtctc cgaacgggat
     5941 tctgacgcgc ggctgcccac gaacttcgat caaggggtcg atcggatcac gcactacgca
     6001 gttggggtca tactggtggc cgtggctcac gtacgtgtcc ccgccgctga tgtagaacca
     6061 attgcagaat acgatcgggt cgtcgtcctc ttcctcgttg atgttcgaag gtaggggtgc
     6121 gccgagggcg tcgcagatca tgcgttgcac cgaaggccag tagagctcga cgtcgtggtt
     6181 tccgatgatg aacactgcac ggtgaccgcg tgcaatgaac cgcccgaggc gctcgaacca
     6241 aagaggatgg tcctcgatga tgcgttgcat cttgaactgg ctcttccact cctcactacc
     6301 caagccacgg gagcgctcga gccactcgat gtgtcgattc tcgttgggga gggctgtgac
     6361 gctatcgaaa tcgaagatgt cgccattgag cagaagctcg atcggcccgt tcgtcttttc
     6421 ctcgatatga tcgaggaatg cggcgaagtc ctggtcgatg aagaactccc gacgcttgta
     6481 tgccatccac agaggtcgat cgggatgagg ctcttccgcc tccgtgagat gcatgtcgga
     6541 gacgacgaac gtatggaggt tcgattccat cggggctcct ccattgtgtc aggcctcgga
     6601 gccggactcc agctaacggg ctccgagctc ggcttccgag ggcggctacc tgctcattta
     6661 ccctgggagg ctaggtgtcg cttgcggcct atgaacagat gggcgagcag taagagggtc
     6721 agtcccgtcg cgaaaatcgt gacgtacagg gtgccgagac cgggccccgc ttccgtgagg
     6781 aagttcgcga tttgcttctt tcccaacatg accggggtga acggatccac ggtcacgggc
     6841 gccctagggt ccagattgtg cccgaaggtg tagagcttgt aatacaaacg cccgaaaccg
     6901 aagagccccg cataggtcgt catcacgagc aagtcgatca tggagctgac tttgccgatg
     6961 accgcagtcc gcagcgcaaa aataatcatc gcggcgaaca ggaacggaat ccagtccagg
     7021 tcgctcagcg ctgctctgtc gagcggcgcc atgccgatgt agtggttcag aacgttgatc
     7081 tcttgaacgt cgttcccgtc gttcccaccc tctaccttgt acgagtagat gtagaggtcg
     7141 agcccctccg gatattgcgg cgccttcatg ttgatgtacc acatgggcgc ggtgaagctc
     7201 agccctacga agatcgtgag aagcgcgagc acgacgcggg cccaagggaa aatgggctcg
     7261 tccaagaatc gatagaatgc gtccattgcc tttgacattg tgcctcctcc tgggccttca
     7321 cttcttaaac gacgtcaccg cgttgcagcg tcctgagccc gctcagccaa gcgagcgtcc
     7381 cgagcaccgt gggccaaccg acaccgaggg cgtagagacc ccagcttcca attcgattgg
     7441 caagatagaa gccgaccggt ccgagaacgc tgagctctgg gtcggcggat gagagcaaag
     7501 ccattcggat gctctggacg gggttgagcg ccgcgagagc gaagacgact ctgggctcca
     7561 tccgccattg cagcatcatc ccagctaagg cgaaatccat cagcgcaatc cccgccaccc
     7621 agatcagcag caagtacatc gtcgcgcgcg ccgagttctg cacgtgggtc gacacccaga
     7681 ggccgagccc gcagaacgac cagaccagga cggagcaaag taggagacag tgtccgatga
     7741 agctccaggc gatctgctgg ccgaacgcga atcggccaaa caaccccacg gcggccatca
     7801 gcagcgcgag aggtagaagc aagacgagga accggacgct cgaaatcgcg ctgaagaaag
     7861 cgccgcggga gatcggctgg ctgaacagga tctccaaagt accgtcgtcc ctcgccttgt
     7921 tcaccacttg gccggtcgcg atcagagaca tcagcggcaa gagcacgagc aaggcgtgca
     7981 cgtacgagac gagcactcta tcggtgccgg tgaacccaaa gaccgtcgac tcgcgcatgc
     8041 cgaccagcag aaacaccccc gcaagcactg catagacgac gacgcagaac agcagccagc
     8101 gagacctgag aacctccgcc agatcgagct gggcgaccgc ccaataggat cgcagctgac
     8161 gattcatgag cctccgtccc agcgttgggg gcggttcatg acctgggcct tggcccagtc
     8221 gatgttctgc gcttcaggtg cggtgccggg aaccgctcga attccgtagc ccatcgggga
     8281 atcttcgacg gggagaaaac ccacctcgga ctcactgagc cagccttcgc cctcgtgatt
     8341 cctgaagtag agcgcatgga tgatgaccgg gtgcgactga aggtagtcga agaggcaacc
     8401 gggatcgtcg aagttcatca cgccgccatc actggtctgg agctgcgcag cgaaacgtgg
     8461 gtcgcccacg tgcatcttgc agtgttggca gacctcgcta tcccagacca ccgggaccgg
     8521 cccctctgga ggcgtaacct cgttgaagcg catgaacccg acgaccacca cgacgagggc
     8581 gagcgcgagc aagagggcct tcatgagctt actggccatc gtcgctcccg ccttgccggt
     8641 cgagctgcac cgcgtcggca tcgcgcacga gcaagttcat gatccgtcct tccaggccat
     8701 ccgagagctg ggcgacgagc tcgagctttt cagcacgggt ggccgtacgt gtccaccagc
     8761 ctgcaacgcc aggctgaaag cccaaactct cgaggtcctc gctgcccgcg ccgttcttca
     8821 tctgtacgtc gatggtgctg agagtcatgc gatccaggta atcctcggtc ggcccgaagt
     8881 aagctagcgt gccctcttcc agcaccatga cttgatcgac cagcgtgcga atctcctcga
     8941 gccgatgcga gcagaggatc agggtcgcgt ccttggttcg ctccgacaac aagtggaata
     9001 gatcgcgtcg cgactgcgtg tcgaggcttg ccgtcggttc gtcgagcacg tagagcgagg
     9061 cttggctggc cagcgcgagc gcgatcaagg tcttctgctt ggcgccaccc gagagattgc
     9121 aaaacgcttg gcgccccacg gccccgaggt cgatgccgac ttccgctccg caggcgagaa
     9181 ccgcgtccgg cgacatctcc cgcactcgag tgatcgcttt gaccacttcg cccacggagg
     9241 cgccgaactt tggcgcgatc tgcggaacgt aggcgatgcg atcagcgagc tccatctggc
     9301 gcttcttctc tccatcgagg agaagctcgc cctcacaatt gagcaagccg aggatgatcc
     9361 ggatcaaggt cgacttcccg gaagcgttgg gaccgatgag gccgaccttg gagccgctcg
     9421 gaatcgtgaa gtcgattccc cgaagcgctt ggactttgcc gaagcgcttt cgcaccgctt
     9481 tgccttcaac gcgcatcgag gattttccaa tctactgcgc tcatacgggg gcgaggatcg
     9541 atcaaggtcg tctcgggctc gatcatcggg aacaaggagc tgatcgcgtc caaaagctcc
     9601 agcgtgattg cgccacggaa gaactgcagc tgcggatacg tgctctcgag ctggctactc
     9661 agccgccgaa gctcgtaggg gatgtcgcca aagccgtcgc gatccatatc gtacccggcg
     9721 tagtcgtcga aagagtttcc cctccactcg acctcgagcg cgttgccgcc accctcgacc
     9781 tgaagctgag agtgattgct cacgaactga ttgccgcgaa aagcattccg cgtttcgctc
     9841 gaatgcatga gcaccgccac gtcggagtga aagaacgcgt tgttctcgaa cgtgttgaag
     9901 tcgtccggct cgagcggagc agtgtccatg taaacgccct tggtgtttgc gatgaaggcg
     9961 ttgtcagcga ccgtcaagtt gcccgactcc ttgataccga gccccattcc gccgggcccg
    10021 gaggagcgcg ccaagaggtt ctcgcgcaga gtgagattgc ggctgtacat gatgaagatg
    10081 ccgacgacgt tgtcgagata gcggttgcct tccacgtggt tctcgtggct gtacatgaag
    10141 tgtgttccgt accggcttcg gaccacggtg ttccgtacga gtcggttgtt gctcgaatac
    10201 caaaccacca tgtcgcggca gccgatgact tggttgtcac gcacctccga gtgccgagtt
    10261 tcccaaaggc gaattccatc gccgcgaagc ccgagcggcc cctcgtccgg gccttgaacc
    10321 acgttgttga ggatcgcggc gcggttggat ttttcgatga gcaagccaaa cgcggcgttc
    10381 tgcacgcgta gaccctcgac acggacgtca tctgcgacca cgtgaaccgc cgcgtccaag
    10441 gtatcgaagc gtccaccgct tccatcgacg gtgaaacctg agagcgagct gccgtcggct
    10501 tgcacctcga tcgtcgtccc ctccccggcc gaacgaacta ccgcatctcg agggccccat
    10561 atctcgacgc tcttcgcgac gctcaatggg cccgagtacg aaccggactc gaggcagagg
    10621 gccgccccct cctcggcacc gtctacgacc gcctgcaggt ttgcgcccgc agcaacgacc
    10681 tcacagtcgt tcggccgcgg cggcggtgcg agcgccgcct ccttgccaag aggcggcgca
    10741 gcttcggtcg cacacgccgc tacggtcatg agcaccagga agcacacaca cgcatggttt
    10801 cgagcgttca gctccaagcc gacgcgtgcg gcgcttcggc tcaactgggt tccaccaaga
    10861 ggtaccccgc catctcgagg tggagggccg agcagaactc ggtgcagtag aacgggaacg
    10921 tgccctcttt gtccgccagg aacgtgatgt cgacgtgctt gccgggttcg aggctgctgt
    10981 gaacgttgta cgaaccgatt gtgaacccat gagtcgcgtc atgggcctgc tccacgttcg
    11041 tgatgtggag gtgcacgttg tcgccctttt tgacgcgaat gatgtccggg gtgaagtggc
    11101 tgcgcaccga ggtcatgtag acgtggacgt ctttgccctc gcgctcgatg cgctcttcct
    11161 tggccttcac ggcgtttgga ctgtgcgcct gggtcacgac gtcatagcca gaaggcttgt
    11221 acacatcgac ggggtccaac ttgtcggcct tgatcatctg cgcgtagtgc ggctctccga
    11281 gcggaagcgg catgtcgtag atgagctgca tcggctcgct attgatgttg atcagctgga
    11341 agttctgggg gagcaaagga ccgactttgg agaagcggtc cagcgcccac ttgttcattg
    11401 cgatcaggta cccgccatcc ggactgaccg tgtcgccctc ggccgctacg atgtgaccga
    11461 cgttgtagtg aaccttgacc ttctcgagaa ccttgaggtc cttgagtgac catttggcca
    11521 ccgtcgattc gatgaaaacg gaggtgtagg cgttgccctt gtcgtcgtac tgagtgtgca
    11581 gcgggccgag gccgatctcg acctggccac gaatgctgtc gttgaacggc aaaatgggca
    11641 caccgtattg gtccttgccg tcgtacttct tggcctcgat gagacccttg agcttctcga
    11701 agctgtacac cgtcgcgtgc gtgtcgagct tgcccgaaac gacgagctcc ttgccatccg
    11761 gcgtgacgtc cactccgtgg gggctcttcg gctcgccaac caggtgcaag aggccttcag
    11821 cattcgtggt ttcgagggaa atcacgtcca tgccagcgat cttcgtcgtc ttgcccgcct
    11881 tgtacaccga ctcggccttc ttgagatcga tgatgtgcag atagtccatg tcgttctgag
    11941 aggcgccgga ctccatgggc gggttgcctt ccatggtgcc gccccaggcc atctcggtgt
    12001 tgaacgagtt gatgaacacc cagccttcgc tgtcgagctt tccagcatcc gcgaggtcct
    12061 gcatgtacgg aggaagctcc atagcgaagg aattcgctcg atcgattttg cccttctcgc
    12121 ggtcgaactt ccagaagatc accgcgccgc ggtacttgtc ttggaagttt tcgatcggct
    12181 cgtagccacc gcccagaggt gccgggtact ggctagactc gacgacgtac tcggtatttg
    12241 gagtgacgaa agttgcgcca tgatcgctct ggatcagcgt ggaggtgacg atctctctgg
    12301 tgacgaaatc gtgaagatcg atcaccgcaa tgcgagcgtt ggccttgtcg ttcacgaaga
    12361 tgaacttgcc gtcgtactcg ccgttggtct cggagagcgc cggatggtgc atgtcacccc
    12421 aggtcatctc cttgccgaaa cggctccctc ccgcgaggac ctcgccggac tgatcgccgt
    12481 agccgtagcc ctgccagggt tccggcgtga agacgccgat gtacttgagt atgcgcatgg
    12541 acggtacgcc gatgacgatc aggtttccgc cgtgaccccc ggaagcgaag gtcagatagg
    12601 cgtccttctt cccggtgggc gtataggtct ggagcgccgc actcacgtcg gcctcggaga
    12661 gcccgcgctg cttcatcaaa tccgccgtcg agctcgccgg tgcctgagac acctgagctg
    12721 cctgctgcgg cccacatcca atgctgacca ccgcagcaca gagagctgcg acacccgatg
    12781 cgcaaagcgc acccatcctc ttgaacctca tcacaactcc tcacttccct cagcgaaagg
    12841 gaaatcctcc aaaacggccc ccctcagcga aaggggagct ctcctcgttt tcgtgaggcc
    12901 acccccaagc gatggggaga cctccacgcc agagcgcagt gcaatatcta agccaggaac
    12961 ctgttacttc gcgaggtcgc gaacgtactt gaccagctcg gccaccacct cgggcttggc
    13021 gtcgagatcc gggttggcgg gcatgctcgg agacttgccg accgcggagc cgccgtacac
    13081 gatgatcttc tgaagatgct cgtcggtgac cgacgcctgc cactcggccg aagtaaaatc
    13141 acgtggtttt ggttcgagcg cagccgagcc ggggccatcg cccatcccgc tttcaccgtg
    13201 gcaaaccgtg cagcggctct ggaagatctc agcggcctct gcgacggggt cgctcgcagg
    13261 ctctgcggct ggcgccggtg cgggggcggg ctctgctgct ttctgctcag cgggcgcggc
    13321 gtccttcggg gtgcatcccg aaatcccgac gatcaatccg atgagcaacc caatccattt
    13381 cgcgcgcata gagcctccta attgggcggc agtctaggct cagaggaggt gggatcaagg
    13441 catattgagg agaaaaaatt gcgcccaaat cgcgccaagc gaaggatttg cgcggcgcgc
    13501 tgaaacccca cttttcgaat tccgacgcgg aaattgtatg tctatactcc gctcgcgctt
    13561 ggtggcgttt cctagtcagg ggaggatgtg atgagaggtt ttgtgaaaat cacggtcatt
    13621 ggtgggcttc tctgcgggct cctcgttgct ggatgcaaga agcaagaagc tgcgcccgcg
    13681 gagcccactc cgagtgaagt ggcaccggcg caagaagtgt cgaacattcc cccgctgaac
    13741 gagaacaaca tcgtcagcat tgcgatcaat tccaaggatc atacgactct ggtcgccgca
    13801 attaaggcgg cagactacgt cacctcggtg gcggcttccg gtccgttaac ggttttcgca
    13861 cccaccaacg aggcattcgc caagcttcca gaggggaccg tcgagaacct ggtcaagcca
    13921 gagaacgtgg ccacgctgag gaacatcctg caataccacg tcaccacgtc ggcacttcag
    13981 ctcgattggt tcaaggacgg ccagaccgtc tcgatggcga acggcaagag gacgaccatg
    14041 aacgtcaagg acggcaaggt gacgatcaac ggggcgaaca tcctcgccac gatccccgct
    14101 gagaacggga tcgtgtatgt gatcgactcc gtgctccttc cgcccgagaa ataggccgcg
    14161 ccgatgacgc tcgactcagc gtggtcgctt gtccgcggcc tgcgggtcgt cgcagctctg
    14221 ctcagcatga cgccgctgtt cgcttgcacg aacggtggcg tttccgatgt cccattggac
    14281 gccggtatgg acgccggtcc gctggaaccc gtggaagacg gcacatattt cgtgcatgac
    14341 gacgacgagc gcgtcgtcat cttgcgcggc atgaacatca tgagctccgc gaaggggcat
    14401 ccagaaaggc tgccgaacct cgaagaa
//
LOCUS       DASRZF010000312        17334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1962321, whole genome shotgun sequence.
ACCESSION   DASRZF010000312 DASRZF010000000
VERSION     DASRZF010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 17334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17334
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1962321"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..1189
                     /locus_tag="VFG22_13795"
     CDS             158..1189
                     /locus_tag="VFG22_13795"
                     /inference="COORDINATES: protein motif:HMM:NF015376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HET6417364.1"
                     /translation="MTRPRWFVHLVLVGALALAWSCTKKPPPAQSSRTLAPGSANQAV
                     QTETPEPLDWSQFDSTSLRNPSDGALRGGVPLPLAAPGLRFNPKRDPKTRFGTVEVVR
                     GLLAAGSRVDEALQGLPITINDLSYAEGGPIPHHRSHQSGRDVDVLFYQLGPDGAPIE
                     SVGAFFDPSGEGVDFRDLSNPSDDVALSLDIPRNWLFLRSLIEDEDAQLQQIYLAEHL
                     RALLLDYARREGEPSAAVERFAEMSCQPSYPHDDHFHFRFFCAPDDIAKGCLDSPPMY
                     PWQLQKLEASGVRPRPLAPNRAEARARIVTHEEAREAAGPMDAEVERWLDRRKNWLKR
                     PHPGRTYCP"
     gene            1225..2370
                     /locus_tag="VFG22_13800"
     CDS             1225..2370
                     /locus_tag="VFG22_13800"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HET6417365.1"
                     /translation="MPDFKWISKHAELEELVDELTRADAYALDTEFHRERTYLPELAV
                     VQLATRERIALVDALAVDVSPLRRALESEAICVMHAGSQDLEILDLVCGTVPKRMFDT
                     QVAALFCGYRTSALGKLVEGFLGVQLDKSAQLSDWTRRPLPEADLRYAASDVAHLLEL
                     RAALVGRLEQRGRLDWAEEEIEEQRIKDRSPADPDTLWWKLRGKTKLGGKARGVAQEL
                     AKWRDATARKQNRPARTVLSDMALLALSQRPARTAEQLRAVRNFDLRRFKHTEELLGA
                     IRRGSELPTSALRLPPKKPENLPSVEGVIALCLAWLSQRAAQEDLDTSVLGTRDDVTR
                     LVLRQPSRLSSGWRDTLVGCDLRSIIEGMAALGVNGTELELVNRAPS"
     gene            2390..3451
                     /locus_tag="VFG22_13805"
     CDS             2390..3451
                     /locus_tag="VFG22_13805"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET6417366.1"
                     /translation="MAVAKGGRIVLLLASVVVIGAGIKLAAPLLVPVLVASFIAIVTA
                     PLVMWLCDRGLPRLLSVLSGLMLDVTAGTVLGFSLAAAVATFTDRAPEYAAMLAGKQE
                     ALELWLTNYGIHLEAIYDFSEPTWMLNFATNMAQSAASFVSQIILVLLIVAFMLFEAT
                     GLREKLGRIATPAQINELSEAAREVNTYLVAKTVMSLLTGVLVFVWCWWRGVDVPVLW
                     GLLAYLLNYIPTVGQIIAAIPAITLALIQFGPGEALVVAAGFGGINLAIGAMEPRVMG
                     QALGLSSLVVLVSMVVWGWLLGPVGALVSAPLTMVIKHALAHSDDLRWLAVLLGRAPR
                     PAGKAGANTADPSDELSRA"
     gene            complement(3438..4436)
                     /gene="meaB"
                     /locus_tag="VFG22_13810"
     CDS             complement(3438..4436)
                     /gene="meaB"
                     /locus_tag="VFG22_13810"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HET6417367.1"
                     /translation="MKRLSAQAYVDGVRGGDRAILARAITLIESEQPAHAELAQEVLE
                     ALLPDTGGAHRVGISGVPGAGKSTFIDALGTMLTSRGHRVAVLAVDPTSSVSGGSILG
                     DKTRMSSLSIDPGAFIRPSPTSGTLGGVARKTRETILLCEAAGFDVVLVETVGVGQSE
                     TIVAGMVDFFVVLLIAGAGDELQGIKRGILEVADLLAINKADGDNRSRAMRAQASYQS
                     ALRLMRGDTVPPVVTCSALEKTGLSELWASVVQSRADLEASGAFQQRRQSQQVRWMWD
                     MVENGLRRALRGDPELSALIAQLEDEVGNGRATPSAAAAQVLRRFAERAGEALRRG"
     gene            complement(4433..6610)
                     /gene="scpA"
                     /locus_tag="VFG22_13815"
     CDS             complement(4433..6610)
                     /gene="scpA"
                     /locus_tag="VFG22_13815"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF006944.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HET6417368.1"
                     /translation="MSRLPDFSTIDFDATQEGGSSSIADWKASAGELAEQRWKTPEGI
                     EVKPVYTAEDLDPVTHLDTMPGLPPYLRGPYSTMYVRRPWTIRQYSGFSTAEASNAFY
                     RRNLAAGQKGLSIAFDLATHRGYDSDHPRVAGDVGMAGVAIDSIKDMRILFDAIPLDQ
                     MSVSMTMNGAVLPILALYVVAAEEQGVDSKQLTGTIQNDILKEFMVRNTYIYPPSPSM
                     RIVADIFKYTAAHMPRFNSISVSGYHMQEAGATQDLELGYTLADGLEYVRTGMAAGLG
                     VDEFAPRISFFWAVGTNFFMEVAKLRAGRLLWAKLIEQFGPKNPKSLALRTHCQTSGW
                     SLTAQDPYNNVVRTCIEAMAATQGHTQSLHTNSLDEAIALPTDFSARIARNTQLYLQL
                     ESGTTKVIDPWGGSYYVERLTHELARAAWAHIKEVEELGGMTKAIEAGLPKLRIEEAA
                     ARTQARIDSGQQTIVGINKYRLEKEDAIEVLKVDNTAVREAQIARLRELRAERDEAAL
                     RPKLEALTKCAETGDGNLLELAIDAARHRGTVGEISDALERVWGRYQATIRSIEGVYR
                     SEVGKDSEAVDRVRARSDEFLLRHGRRPRILVAKMGQDGHDRGQKVIATAFADLGFDV
                     DIGPLFQTPEESAKQAIENDVHVVGASSLAAGHLTLVPQLKQALRDQGREDILVVVGG
                     VIPPSDYDALLKAGAVAIFGPGTVIAEAAIELLEKIDAQLGGS"
     gene            complement(6607..8655)
                     /locus_tag="VFG22_13820"
     CDS             complement(6607..8655)
                     /locus_tag="VFG22_13820"
                     /inference="COORDINATES: protein motif:HMM:NF013781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase subunit beta"
                     /protein_id="HET6417369.1"
                     /translation="MVNPDERMNAFPPLSKADWIAKVEADLKSVSLDALRSTLPGGLK
                     LEPLYTADDARDLRAPGLPGVFPYLRGAAPLGGWTIRQEYDDPRPSVCKKLIAEDLER
                     GVEALYLRLGPRNGCRVLTIDSLDGLLESVHLEKTSIWLDAGSDALAVAAGLLAVAKR
                     RGVSYALLDGCFGFDPVGLIAEEGRLQGGLRARFSELRDLASWCSQNAEGLRAVEVSS
                     EPYHGGGASTVQELAYSIATGIEYLRQLVDGGMGVDSAARQIAFSYSVSGDFFTEIAK
                     LRAARWLWAKVVVAAGAEPNAGAMHIHARTSRFTKAQRDPWVNMLRATAECTAAVLGG
                     ARSVATQPFDCAIGPADELAQRVARNTQVVLREEAHLGKVADPAGGSWFVESLTNDLA
                     RAAWAELQSIEGGGGIVAALGSGKLIDAVSEVADAREQDLARRKTPIVGVSEFPNLRE
                     DAVQREPVSEEEVQASFKASLDALELGAHRDRLLALVKLANDLERPAGALTEACVAAA
                     SDGADMYSIATVLQHGQPDFHLEPILQWRQSEIWEELRARSDRARSPLLAFLANLGPT
                     PSHKARSTWTQNLLAAAGIDAKGNAGFSEASSLASAWNESGASLAVIAGSDEDYETML
                     EPAIAELKTAGCPVVLVAGRPGDRESRLREAGASDFIFVGADVLSVMSRVLDSIGVKP
                     "
     gene            complement(8701..11199)
                     /locus_tag="VFG22_13825"
     CDS             complement(8701..11199)
                     /locus_tag="VFG22_13825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417370.1"
                     /translation="MDEGTYDLEDGNESAARPKRPGLPVEPRRLLRILAEHRKPLLKA
                     FLIAAGVSILGSFFVPRTFESSAQLLYEGTPVLERKEQETTADASAASAMAQERLREV
                     RDRINWSGSLTNLERHLDATVEDELRTSLRISARAGDAEEAHAMATAMLEVFLEHQAS
                     FNARRLEILSAETRVALESAKARRDQATQEYDAFRRKSGKPNLLQEQDQLLKRAALLR
                     SQADEAAVEVLAQQARIEELERAQEDLPRQIVSSAKKGSPIDSPLSEARSELAAARAS
                     LSDQHPTVQALTQRVESLQAQRKGEKSELGEQTVISNPARATVDQEIATARAAFAAAK
                     ERESALRVLLKANKAETESLAPEEGEARRIVGELEAASDRVKSLSERSAVLRDAAMGP
                     LTGFRVLSSPAVPEASTPSPLYVALLILLPLLTVTIFALVVITRQLRSLTLEAPREVA
                     WWGNGPVLGTSVWPRDSTALDTFVDELEDHGIYGAGRTLVVPATDAEREIALSFAMRL
                     AQAPWLAAAILDIDERASSRPYGASLVTPPPGGYGRHLTPPPVAPPRRLSAQGMPSMP
                     PGRVIQTPPPQSSTPGPSPSRPPRKKTMVGLPAVQSSHPPSAAASSTGSPTTSDAPSS
                     SSGPEPFRRKRRARASVRMVVPVNESLGTSGAASSARDSAPDEEAFLLTRPVPIATEQ
                     TEQTEPRVGRAVHVATEMPPASASDAVMRAAVRLLGNGEDDITGPRPSNPPNSRVVGE
                     VTGVALAWNGPLSGPVLRRAARLAHRVMVVVSSGMSAVDLARIHTRLGRENGVGYVLV
                     NVSDAYVDLKDRVGPVEEFWAGLRDADPLDSRRP"
     gene            11291..11839
                     /locus_tag="VFG22_13830"
     CDS             11291..11839
                     /locus_tag="VFG22_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417371.1"
                     /translation="MRPWVEKLLAAWGIAWVSLLLGRALWRLTPLAMEPWTEDMMTPA
                     QKGIYIAWLVANAYLEGYRGFQLRFSPRVVSRAIYLAQNPRPLWVILALPFCMSLFHS
                     TRRQKTVSWVFIGAIVLLVWSVRSLPQPWRGIVDGGVVLGLAWGLAVIWWLFLRYLLG
                     AKVQLPPDLPTPQLVEKQPAPG"
     gene            12030..12935
                     /locus_tag="VFG22_13835"
     CDS             12030..12935
                     /locus_tag="VFG22_13835"
                     /inference="COORDINATES: protein motif:HMM:NF015376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HET6417372.1"
                     /translation="MPWVCGLIAAFLVWLSGSFGTSASAELPMTGDPVLREAQHAESH
                     AEPSDEDLERIARMPRSVAPSDLEPNPSVSVGYPNRGLLRFGMRINDDKDLRVKVGSL
                     DSRHGTGELVRLIEHAAHEVAFRYPRAELTVGDLSRSGGGRFRPHVSHQSGRDVDIGF
                     YMLDRRATPVNVHMFVLFNNQGIGRQWGNLYKFDAARNWALIESLLSHPTTDVQHIFV
                     SNSIKRRLLDQAIKEGANPDLLMRAKRVITQPRHGAPHRSHFHVRVYCADDDQPMCKD
                     RPPFYAWHQSAHEAPAVSALSPNDS"
     gene            12932..13870
                     /locus_tag="VFG22_13840"
     CDS             12932..13870
                     /locus_tag="VFG22_13840"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6417373.1"
                     /translation="MSWKGHFWTLGPFLRHNLLPPEVPEWQRWETSVRDGVVGTLDLS
                     GRLSQDGQADTIVVIVHGLGGSATSYYARQAAQAAARVGIDSLRLNLRGADRGGADYY
                     HAGLTDDLDAALKSPALARYQNIFLLGYSLGGNMMLRYLAGSPDPRVRAAAAVCSPID
                     LKPGARAIDRPRGLFYRRHILSALKDIYSQVAARREVPLPLEQAVRIDTIESWDERVI
                     SPRHGFAGAEDYWARTSACNILEEIKTPTLFIASERDPMVPIDTVLPWLQTASQLRWV
                     VTPKGGHVGFPQKVNLGLGSGGTVEDQILQWMQAPT"
     gene            13948..16953
                     /locus_tag="VFG22_13845"
     CDS             13948..16953
                     /locus_tag="VFG22_13845"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HET6417374.1"
                     /translation="MPQRPASCPETRQVEPPLANVAPEHETLAYWLERGEARGGLDKL
                     LLSPAGVRRHNAALREPRDGEPIGQADLRAPVDRSTLDAEVEERLAYLRKRIEAAELV
                     NASGHAMSAAELAYFALPASAPSVQAVEQWNLIEDLTPLRCGPYDGGLHTRPVDLDFD
                     RNRCSTMRQGEIVQVLARWPNGMALARTPYALGWVASDALSSPLDRELLEDRLERKEL
                     LPFTRRALLSEAFSMLGEPYGWGGKDGGYDCSRFLLEVFGRFGIDLPRHSARQAEAGT
                     FSLDVSEVEDLNEKKLLIEAAARRGIVLLHFPGHIMLYLGTTEAGTPMAMHAFSEYLT
                     PCTETGLETVNRVDRVAVSDLSLGAGSSRNDFLRRITRVTVLGGAPGPALSADAELRA
                     SAPISFPEDRCADTKRFAIFHTPQRPNPSQPLRVIVTSDADPGPASLVLFAPDGSQVI
                     PDSHRLDGPPHGRWIEVEAPTAGEWTALFADGDRVHACERITVRKHPAQPVRRAFQAP
                     AWEPRRQWGHDTENLYAAFVEQLFREPAGEDVTWSSLQELIGSPESNLLYDHNLPGED
                     ALLDLEPDCADLPYFLRAYFAWKLGLPFAYRTCTRGTKASPPVCEPTVLSNLDSVPDS
                     NEADAFRRFVRRMAGTVHSSSPRTVPSDDQTDLYPVRLNRRAMPPGTVFADPYGHVLV
                     VARWKPQGVTDYGVLMGADAQPDGTVGRRRFWRGSFLFTPNTELVGAGFKAWRPVRYD
                     PSVLPGAVTGNADGNIEGAPEPAAAPQPWAIPSNEELRKAGGTRAWSDVQYDGSADDF
                     YATIEGLINPRALDPVRMQISLVDALEESVQRRLSSVQNGEDFMKSQEYAPIDMPHGA
                     ALFLTSGPWEDYSTPSRDMRLLISIDAVMTFPDSVGAHPERFGIPEGEQASAAQQVRD
                     ALRAELAQRSFEYTRSDGSAWALTLGDLVARAKPMEMAYNPNDCPELRWGAAEGSQEH
                     ATCKRRAPAAQQSRMRTYRNWFAKRERPG"
     gene            complement(16967..>17334)
                     /locus_tag="VFG22_13850"
     CDS             complement(16967..>17334)
                     /locus_tag="VFG22_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417375.1"
                     /translation="STAALDCIKRRIGALALRPLEDTAQSETASTVIEIKYEPPLTIE
                     TAQGTPEPELRNVRDPLPTPESLPLSGTPIQESPGNPPKGGEAKQRDIDDPEPRYPRG
                     PKPRTTDGYEVDDNVEVWR"
BASE COUNT         3027 a         5711 c         5408 g         3188 t
ORIGIN      
        1 gctcaaacct gagctatact ccccgcgtca agatgcaagt cgctcggacc cgcctacaag
       61 tagacgttcg cagacagcag ctccgcgagc tcgggctcga gctgctcgtc cagatgtcag
      121 gcgtcgtgtt ctccgtcgtc ctccaccccg tgacaccgtg acgaggcccc gctggttcgt
      181 tcacctcgtc ctcgtcggcg cattggcgct tgcctggagc tgcacgaaaa agcccccgcc
      241 cgcccaatct tcgcgaaccc tcgctcccgg gtcagcaaac caggcggtac agacggaaac
      301 gcccgaaccg ctcgactgga gtcaattcga ctcgacgtcc ctgaggaacc ccagcgatgg
      361 agctcttcgg ggcggcgttc cacttccgtt ggccgccccc gggctgcggt tcaacccgaa
      421 gagggacccc aagacgcgtt tcggcacggt cgaggtcgtt cgcgggctcc tcgctgcggg
      481 cagccgagtc gacgaggcgc ttcaggggct tccgatcacg atcaacgacc tcagctatgc
      541 ggaaggcggg ccgatcccac atcatcgctc gcaccaaagc ggccgcgacg tcgatgtgct
      601 gttctatcaa ctgggacctg acggagcccc gattgaaagt gtgggcgcgt ttttcgaccc
      661 cagcggtgaa ggcgtcgatt ttcgcgacct atcaaatcct tcggacgatg tcgccctcag
      721 cctcgacatc ccacgcaact ggctgtttct tcgctcgctc atcgaagacg aagacgcgca
      781 gcttcagcaa atctacctgg cagagcatct gcgcgcgctt ctgctcgact acgcgcgtcg
      841 cgaaggggag cccagcgctg cagtcgaacg tttcgccgag atgagctgcc aacccagtta
      901 tccgcacgac gatcacttcc atttccgatt cttctgcgct ccggacgaca tcgccaaggg
      961 ctgcctagac tccccaccca tgtatccgtg gcagctccag aagctggagg catccggggt
     1021 tcgcccgcgt ccccttgccc cgaaccgcgc cgaagcgagg gcccggatcg tcacgcacga
     1081 agaggctcgg gaagcggcag gacccatgga cgcggaagtc gaacgctggc tcgatcgacg
     1141 aaagaactgg ctgaagcggc cccatccggg acgaacctat tgtccttgag cgggcggcca
     1201 tccctcgatg cgctagagtg gcccgtgccc gatttcaaat ggatcagcaa gcatgcggag
     1261 ctcgaagagc tcgtcgatga gctgactcga gccgacgcat atgcactcga taccgagttt
     1321 caccgggagc gaacctatct gcccgagctt gccgtcgttc agctcgcgac gcgtgagcgc
     1381 atcgcactcg tcgatgcgct cgcggtcgac gtgagcccac ttcgccgagc gctcgaaagc
     1441 gaggcgatct gcgtcatgca tgcgggttct caggacctcg agattctcga tctagtgtgc
     1501 ggaacggtgc ccaagcgcat gttcgacacc caggtcgctg cccttttctg cggctatcgt
     1561 acgagcgcgc tcgggaaact cgttgaagga ttcctggggg tccagctcga caagagcgcg
     1621 cagctgagcg actggacacg ccgccccctg cccgaagcag acctgcgcta cgcggcctcc
     1681 gatgtcgcgc atctgctcga gctgcgcgcg gccctcgtgg gtcgcctcga gcagcgcgga
     1741 cgcctcgatt gggccgagga agagatcgaa gagcagcgca tcaaggaccg ttcaccagcc
     1801 gatcctgaca cgctatggtg gaagcttcgt ggcaaaacga agctcggggg caaagcgcgg
     1861 ggcgtggcgc aggagctcgc caagtggcgc gacgcaacgg ccaggaagca gaaccgccca
     1921 gctcggacgg tgctctcgga tatggccctt ctcgccctgt cccagcgccc ggcacgaacc
     1981 gccgagcaac taagagccgt tcgcaacttc gacctgcgcc gcttcaagca taccgaggag
     2041 cttctcgggg ccatccggcg aggtagcgag ctcccgacga gcgcgctccg cctacccccc
     2101 aaaaagcccg agaacctgcc cagtgtcgaa ggcgtgatcg cgctctgtct cgcgtggctc
     2161 agccagcgag cggcccaaga ggatctcgac acgagcgtgc tgggcacacg ggacgacgtg
     2221 actcgcctgg ttcttcgcca gccgtcccgt ctttcgagcg gctggcgcga caccctggtg
     2281 ggatgcgact tgcgttcgat catcgagggg atggccgcgc tcggcgtcaa cgggaccgag
     2341 ctcgagctcg tcaatcgcgc accctcctga ttcgcgctac attgtcggaa tggctgtggc
     2401 caaaggcggg cgtatcgttt tgctcctggc atcggtcgtc gtgatcgggg cgggcatcaa
     2461 gctcgcggcg cccctgctcg tgcccgtgct cgtggcttcg ttcatcgcga tcgtaaccgc
     2521 tccgctggtg atgtggcttt gcgatcgcgg cctcccccgg cttctctcgg tgctctcagg
     2581 gctcatgctg gatgtcacgg ccggtactgt cctcgggttc tctctcgctg cagcggtcgc
     2641 gaccttcacc gatcgcgccc cggagtacgc ggcaatgctc gccgggaagc aggaagccct
     2701 cgagctctgg ctcaccaatt acgggatcca cttggaagcg atctacgact tctcagaacc
     2761 tacgtggatg ctgaattttg cgacgaacat ggcgcagtcc gccgcatcgt tcgtgtcgca
     2821 gatcattctg gtcctcttga tcgtagcgtt catgcttttc gaagcgacag gtcttcgtga
     2881 gaagctaggc agaatcgcga ctcccgctca gatcaacgag ctctcggagg ccgcgcgcga
     2941 ggtcaacacg tacctggtgg cgaagaccgt gatgagcctt cttaccggtg tgctggtgtt
     3001 cgtctggtgc tggtggcgcg gcgtggatgt ccctgtcctc tggggattgt tggcgtacct
     3061 gctcaactac atcccgaccg ttggtcagat catcgcagcg attcccgcga tcacgctcgc
     3121 cctcatacag ttcggcccgg gggaggcttt ggtcgtcgcg gcgggcttcg gcggcatcaa
     3181 tttggcgatc ggcgcgatgg aaccccgggt catgggtcag gcgcttggtc tgtcctccct
     3241 ggtcgttctc gtctcgatgg tggtctgggg ctggttgctc ggccccgtcg gcgcgctggt
     3301 ctccgcaccg ctcacgatgg tgatcaagca tgccttggcg cacagcgacg atttacgatg
     3361 gctggccgtc ctcctcggtc gcgctccgag gcccgcgggc aaggccggcg caaacaccgc
     3421 agacccttca gacgagctca gccgcgcctg agagcttcgc cggctcgttc tgcaaaccgg
     3481 cgcagcacct gcgccgcggc cgcgctcggc gtcgctcgac cattgcccac ctcgtcttcg
     3541 agctgggcga tcagcgcaga cagctcggga tcccctcgaa gcgcacgccg caagccgttc
     3601 tccaccatgt cccacatcca tcggacctgc tgcgactggc gcctctgctg aaacgcgccc
     3661 gacgcttcca agtccgcgcg gctctgcacc accgaggccc agagctcgct caggccggtc
     3721 ttctcgagcg cgctgcaggt cacgaccggc gggaccgtgt cgccacgcat gagccgtaat
     3781 gcgctctgat aagaggcctg cgcccgcatg gcacgcgagc gattgtcgcc gtcggccttg
     3841 ttgatcgcca gcaagtctgc aacctcgaga atgccgcgct taatgccttg gagctcgtcg
     3901 ccggcgccgg cgatcaggag cacgacgaag aaatcgacca tcccggcgac gatggtctcc
     3961 gactgcccca cgccaaccgt ttcgacgagc actacgtcga agcccgccgc ttcgcacaag
     4021 agaatcgtct cgcgagtctt gcgcgccacc ccacccaggg taccgctcgt cggcgagggg
     4081 cggatgaagg cgcccgggtc gatgctcagc gacgacattc gcgttttgtc gcccaagatg
     4141 cttccgccgc tgacgctgct cgtcggatcg acggccagga cggcgactcg atgccctcga
     4201 gacgtgagca tcgttccgag cgcgtcgatg aatgtgctct tcccggcgcc cgggacgccg
     4261 ctgatgccta cccggtgtgc cccaccggta tcgggtagca gtgcttcgag aacttcctgc
     4321 gcaagctcgg catgggccgg ttgctcgctc tcaatcaagg tgatcgctcg cgccaggatc
     4381 gcacgatcac cgccccgcac tccgtcgaca taagcttgcg cggaaaggcg tttcacgacc
     4441 ccccgagttg cgcatcgatc ttttcgagga gctcgatcgc tgcttctgca atcaccgttc
     4501 caggaccgaa tatcgcgacg gcgccggctt tcagcagcgc gtcgtagtcc gagggaggga
     4561 tgacgccacc aaccacgacg agaatgtctt cgcggccctg gtctctcagt gcctgcttca
     4621 actgcggcac cagcgtcaag tgaccggcgg ccagggagct tgcaccgacg acatgcacgt
     4681 cgttctcgat cgcttgcttc gcgctctcct ccggggtctg aaacagcgga ccgatgtcga
     4741 catcgaaccc gaggtctgca aacgcggtcg cgatcacttt ctgaccccgg tcgtggccat
     4801 cttggcccat cttggcgacc agaatacgtg gccgacggcc gtgcctcaga aggaactcgt
     4861 cgctccttgc gcgcacgcgg tctacggcct ccgaatcttt gcccacttcg cttcgataca
     4921 cgccttcgat actccgaatc gtcgcttgat aacggcccca gacgcgctcg agcgcgtcac
     4981 tgatctctcc gacggtaccc cgatgcctgg cggcgtcgat cgccaactcg agcagattgc
     5041 cgtcaccagt ctccgcgcac ttcgtcaatg cctcaagttt cggtcggagg gcggcttcgt
     5101 cgcgctccgc tcgcagctct cgaagacgcg cgatttgcgc ttcccggacc gcggtattgt
     5161 cgaccttcag aacctcgatc gcgtcttctt tttcgagccg gtacttgttg attcctacga
     5221 tagtttgctg cccggagtcg atgcgtgcct gggttcgcgc cgcagcctcc tcgatccgaa
     5281 gcttggggag cccggcttcg atcgccttgg tcatcccacc gagctcctcg acctctttga
     5341 tgtgcgccca ggctgcccga gcaagctcgt gggtcaatcg ctcgacgtag taactcccgc
     5401 cccatgggtc gatgaccttg gtggtgccac tctcgagctg aagatagagc tgggtgttgc
     5461 gagcaatgcg cgcgctgaaa tccgtgggaa gcgcgatcgc ctcgtcgagc gagttggtgt
     5521 ggagactctg agtgtgaccc tgcgtcgccg ccatggcttc gatgcacgtg cgaaccacgt
     5581 tattgtaggg gtcttgcgcc gtgagactcc atcctgaagt ttggcaatgc gtgcggagcg
     5641 cgaggctctt cgggttcttt ggtccgaact gctcgatcaa cttggcccag aggaggcgcc
     5701 cagccctgag ctttgcgacc tccatgaaga agttcgtgcc cacggcccaa aagaagctga
     5761 tgcggggcgc gaactcatcg acaccgaggc ccgcggccat ccccgtacgg acgtactcga
     5821 gaccgtccgc cagcgtgtag ccgagctcca agtcctgtgt cgctccagcc tcctgcatgt
     5881 gatagccgct cacgctgatg ctgttgaagc ggggcatgtg ggcggcggtg tacttgaaga
     5941 tgtccgcaac gatccgcatc gaaggcgacg gcggatagat gtacgtgttg cgcaccatga
     6001 actctttgag aatgtcgttc tgaatggtgc cggtgagctg cttggaatcg acgccctgct
     6061 cttcggccgc cacgacatag agcgcaagaa tgggcaggac cgccccgttc atggtcatcg
     6121 aaacgctcat ttggtcgagc gggatggcat cgaagaggat gcgcatgtcc ttgatcgaat
     6181 cgatcgcgac ccccgccatg ccgacgtccc ctgccaccct cgggtgatcg gagtcgtagc
     6241 cacggtgagt agccaaatca aatgcgatcg agaggccctt ctggcccgcg gcgaggttgc
     6301 gtcgataaaa cgcattgctg gcttcggccg tcgagaagcc ggagtactgg cgaatcgtcc
     6361 agggccggcg aacatacatc gtcgagtacg gtccacgcag gtacggcgga aggccgggca
     6421 tcgtgtcgag atgcgtaacg ggatcgagat cctctgccgt gtagacgggc ttgacctcga
     6481 tgccttcggg cgttttccac cgttgctcgg ccagctctcc ggccgacgcc ttccagtccg
     6541 caatcgaact gctgccacct tcctgcgtgg cgtcgaaatc gatcgtgctg aagtcgggaa
     6601 ggcggctcat ggtttcaccc cgatcgaatc gagaacccgg ctcatcacac tgagcacgtc
     6661 agcgcccacg aagatgaaat cgctcgcccc ggcctcgcga agtctgctct ctcggtcgcc
     6721 cgggcggccc gcgaccagaa cgacggggca cccagctgtc ttgagctcag cgatcgccgg
     6781 ctcgagcatc gtctcgtagt cctcgtcgct gcctgcgatc accgccagcg acgccccgga
     6841 ctcgttccat gcggacgcga gtgagctcgc ctcactgaaa cccgcattac ctttcgcgtc
     6901 gatcccggcc gcggccagca ggttctgggt ccatgtagaa cgcgccttgt gtgatggagt
     6961 cggtccgagg ttcgcgagga atgcgagcag cggggagcgg gcgcgatcgc tcctcgctcg
     7021 gagctcctcc cagatttctg actgcctcca ctgcagaatc ggctcgaggt gaaagtcagg
     7081 ctgtccgtgc tgcagcacgg tggcgatgct gtacatgtcg gccccgtcgc tggccgccgc
     7141 aacgcaggcc tcggtaaggg cgccagcggg gcgttccaga tcgttggcga gcttcacgag
     7201 cgcgagtaag cggtctcggt gcgcaccgag ctcgagcgcg tcgagcgacg ccttgaacga
     7261 ggcctgcact tcctcttcag aaacgggctc gcgctgaacc gcgtcttctc ggagatttgg
     7321 aaattcgctc actcccacga taggcgtctt gcgccgggcg agatcttgct cccgggcatc
     7381 cgcgacttcg ctcactgcat cgatgagctt gcccgacccg agcgcggcaa cgattccccc
     7441 cccgccctcg atcgattgga gctcggccca ggcggcgcga gcaaggtcgt tcgtcagtga
     7501 ttcgacgaac cagctgcccc ctgccggatc ggcaaccttg ccgagatggg cctcctcccg
     7561 aagcacgact tgggtgttgc gcgctaccct ctgcgccagc tcatcggccg gcccaatcgc
     7621 acaatcgaag ggctgcgtcg cgacactccg cgcacctccg agcactgccg cggtacattc
     7681 tgccgttgcg cgaagcatgt tgacccaggg gtcccgctgg gccttcgtga agcgcgaagt
     7741 tcgggcgtgt atgtgcatgg ctcccgcgtt gggctcagcg cccgccgcga cgaccacctt
     7801 ggcccagagc catcgtgccg cgcgaagctt cgcaatctca gtaaaaaaat cgccagacac
     7861 cgagtaactg aaagcgattt ggcgagccgc cgagtcgacg cccatgccgc cgtcgacgag
     7921 ctgcctgaga tattcgatgc cggtcgcgat gctgtacgcc agctcctgaa ccgtgctcgc
     7981 cccgccgcca tggtagggct cgctcgacac ctctacggcg cgcaaaccct cagcattctg
     8041 agagcaccag cttgccaagt cgcgcagctc cgagaacctg gctcgaaggc ctccttgcag
     8101 cctcccctcc tccgcaatta ggcccaccgg atcgaaaccg aagcagccgt ccagcaacgc
     8161 gtacgaaacg ccgcggcgct ttgcgacggc gagcaggccg gccgccacgg cgagcgcatc
     8221 ggaaccggca tcgagccaga tcgaggtctt ctcgagatgc accgactcga gaagcccgtc
     8281 gaggctatcg atggtcagca ccctacagcc gttgcggggt ccgagacgaa gatagagcgc
     8341 ttcgacaccg cgctccaggt cctctgcgat cagtttcttg cagacgctcg gacgcgggtc
     8401 gtcgtattct tggcggatcg tccagccgcc gagcggcgcc gcgccacgca gataagggaa
     8461 cacgccaggc agtccgggag cgcggaggtc ccttgcatca tcagccgtgt ataacggttc
     8521 gagcttcaga ccacctggga gggtcgaacg cagcgcatcg agcgagacgc tcttgaggtc
     8581 tgcctccacc ttggcgatcc agtcggcctt gctcagaggt ggaaacgcat tcattcgctc
     8641 gtcgggattc accacagcga gaactaaata gactagagag attgcagccg caacgcgcgc
     8701 ctagggccgc ctcgaatcca aggggtccgc gtcgcgcagc cctgcccaga actcctctac
     8761 cggtccgacg cggtccttga ggtccacata ggcgtcgctc acgttgacca gcacgtagcc
     8821 cacgccattc tcacggccta gacgcgtgtg aatccgcgct agatcgacag cgctcatccc
     8881 ggaggacacg accaccatca cgcggtgggc gagacgcgcc gcacgccgaa gcacgggacc
     8941 gctgagcggc ccgttccacg cgagcgccac gccggtgact tcaccgacca cgcgcgaatt
     9001 cggcgggttc gagggacgcg ggcccgtgat gtcgtcttcg ccgttgccga gaagcctcac
     9061 tgcggcgcgc atgacggcat cggatgcact ggcggggggc atttccgtgg ctacgtgaac
     9121 tgcgcgcccg acgcgcggct cggtctgctc cgtctgctcc gtcgcgatgg gaacgggcct
     9181 cgtcaagagg aacgcctcct cgtcgggggc cgagtcgcgc gccgaggaag ctgcaccgga
     9241 agtgccaagg ctctcgttca cgggtacgac catgcgcacg ctggctcgcg cgcgacgttt
     9301 acgccgaaat ggctcgggtc ctgaagacga gctgggagcg tcggacgttg tcggcgagcc
     9361 cgtgctcgag gctgcggccg atggcggatg ggacgactgt accgccggca agccgaccat
     9421 cgtcttcttc cggggcggcc tcgacggtga aggccccgga gtgctcgact gaggaggcgg
     9481 tgtttgtatg acgcgccccg gaggcataga gggcattcct tgtgccgaga gtcgtcgcgg
     9541 cggagccact ggcggtgggg tcaggtgccg cccatatcca ccgggcggtg gcgtcaccaa
     9601 ggacgcaccg taaggcctgc tgctggcgcg ctcatcgata tcgaggatcg ccgctgcaag
     9661 ccacggcgct tgggcaaggc gcatcgcgaa cgaaagcgcg atctctcgtt ccgcgtccgt
     9721 ggccggcacc accagtgtgc gcccggctcc gtagattcca tgatcctcga gctcgtcgac
     9781 gaaggtatcg agggcggtgg aatcacgggg ccagacgctc gttccgagca ccggcccgtt
     9841 cccccaccag gctacttccc gcggcgcctc caaagtcaag ctccgcagct ggcgcgtgat
     9901 gaccaccagc gcgaaaatgg tgaccgtgag caggggcaat aagatgagaa gcgcaacata
     9961 caacggagag ggcgtcgacg cctcgggcac ggccggcgaa gacaggacgc ggaagccagt
    10021 cagcgggccc atcgcagcat cacgaagcac ggcggagcgc tcgctcagcg acttcacgcg
    10081 atcgctcgct gcttccagct cgcccacgat ccggcgcgcc tcgccctctt cgggtgcaag
    10141 cgactcggtc tcggccttgt tggctttcaa gaggacccgg agcgcggatt cccgttcttt
    10201 cgctgcggca aaggccgcgc gcgccgtggc gatttcttgg tcgaccgttg cgcgcgctgg
    10261 gttcgagatg accgtctgct caccgagctc gctcttctcg ccctttctct gcgcttgcag
    10321 gctctcgact cgctgcgtga gggcctgaac cgtcggatgc tgatcactca gggacgcgcg
    10381 ggcagccgcg agctcggaac gcgcctcgga gagcggacta tcgatcggcg agcccttctt
    10441 ggcgctcgag acgatctgcc gagggagatc ctcctgagcc ctctcgagct cttcgattcg
    10501 ggcctgctgt gcaagcacct cgacggcggc ttcatccgct tgcgaacgaa gcagcgccgc
    10561 acgcttcaag agttgatcct gctcctggag caaattgggc ttgccactct tccggcgaaa
    10621 ggcgtcgtat tcttgagtgg cctggtctcg ccgggccttg gcgctttcga gtgcaacgcg
    10681 cgtctcggcg ctgaggatct cgagcctccg cgcgttgaag ctcgcttgat gttcgagaaa
    10741 gacctcgagc atcgcggtcg ccatggcgtg cgcctcctct gcatcacctg cccgcgcgga
    10801 gatgcgcagc gaggttcgca attcgtcttc aaccgtcgca tcgaggtggc gttcgagatt
    10861 cgtgagcgaa ccgctccaat tgatgcggtc gcgcacctcg cgaagtcgtt cctgcgccat
    10921 cgctgaagca gcggaagcat ctgctgtcgt ctcctgctcc ttgcgctcta gaacgggtgt
    10981 tccctcgtac aagagctgcg cggaggactc gaacgtgcgc ggcacgaaga acgaccccag
    11041 gatggagact cccgcggcga tcaggaatgc cttgagcagc ggctttcggt gctccgcgag
    11101 aatgcggagg agccgccgcg gctcgaccgg gaggccggga cgcttgggcc tggcggccga
    11161 ctcattcccg tcttctaggt cgtatgtgcc ctcgtccaag tcgctccttc taccctaaag
    11221 agcatggcgg tgcagacgcc ccttgggcaa gtcgacatcc ttagtgagac tgctaacttc
    11281 gcggccgacg atgcgcccat gggtcgaaaa gctattggcc gcctggggaa tcgcctgggt
    11341 ttcgctgctg ctcggaaggg cgctctggag gctcactccg ctggcgatgg aaccctggac
    11401 cgaggacatg atgacccctg cacaaaaggg gatctacatc gcctggctcg tggccaatgc
    11461 ctatctagag gggtaccggg gattccagct gcgattctcg ccccgtgtcg tttcgcgcgc
    11521 catctatctt gcgcagaatc cgaggcccct ctgggtgatc ttggcccttc cgttttgcat
    11581 gagcctcttc cactccacac gtcgccaaaa gacggtgagc tgggtcttca tcggagcaat
    11641 cgtgctcctg gtttggtctg tcagatcgct cccgcagccc tggcgaggga tcgtcgacgg
    11701 gggcgtcgtc ctcggccttg cctgggggct tgctgtgatt tggtggctat ttctgcgcta
    11761 ccttctgggt gcaaaagtcc agctaccgcc cgatttgccg actccacagc tggttgaaaa
    11821 acagcccgcg cccggttgat ctgtcgtttt tttccataaa tctgcccaga tggataaaga
    11881 aaacgctata aaaagcaggc tgaacgggct ctcagccttg gcacctgggt tgactaatgg
    11941 tcaaactggc acttgagggg atacggggca cactgggtct cgccaaaatg ggaaagcagc
    12001 acacaaatgg gggaacgaga tctaggctgg tgccctgggt ctgtggccta attgcggcgt
    12061 tcttggtttg gctctcggga tcgttcggca cctccgcgtc cgccgagctt ccgatgaccg
    12121 gcgaccccgt cctccgcgaa gcgcagcacg ccgagtcgca cgccgagccc agcgacgagg
    12181 acctcgagag gattgcccgg atgcctcgca gcgtcgcgcc ctcagacctc gagccgaacc
    12241 cgagcgtctc ggtcggctac ccgaaccgcg gactgcttcg cttcggaatg cgcatcaacg
    12301 acgacaaaga tctgcgcgtg aaagtcgggt cgctcgactc tcggcacggc accggggagc
    12361 tggttcgctt gatcgaacat gccgcccatg aagtcgcttt ccgctacccg cgcgccgagc
    12421 tcacggtggg tgacctctct cgatctgggg gcggtcgatt ccggccgcac gtttcgcacc
    12481 agagcggccg tgatgtcgac atcgggttct acatgctcga tcggcgcgca acgccggtga
    12541 acgtgcacat gttcgttctg ttcaacaatc aaggcatcgg taggcaatgg ggcaacctct
    12601 acaagttcga tgcggcacgc aactgggcgc tcatcgaatc attgctcagc catcctacga
    12661 ccgacgttca gcacatcttc gtatcgaatt cgatcaaacg tcgcctcctc gatcaagcga
    12721 tcaaggaagg cgcgaacccg gacttgctga tgcgcgcgaa gcgggtgatc acgcagccgc
    12781 gccacggcgc tccgcaccgc agtcacttcc acgttcgggt ctactgcgcg gatgacgatc
    12841 agccgatgtg caaggaccgc ccgcccttct acgcgtggca ccaaagcgcc cacgaagcac
    12901 ccgccgtcag cgctctttcg cccaatgatt catgagctgg aagggtcact tctggaccct
    12961 cggcccgttt cttcgtcata acctgctccc gccagaggtc ccagaatggc aacgctggga
    13021 aacgagtgtg cgggacggtg tggtcgggac gctcgatctg agcgggcgac tcagccagga
    13081 tgggcaggcc gacaccatcg tggtgatcgt gcatgggctc ggaggcagcg cgacgagcta
    13141 ctacgcgcga caagcagccc aggctgctgc ccgtgtcggg atcgatagct tacggctcaa
    13201 ccttcgcggt gcagatcgcg gcggagcaga ctactaccac gctgggctca ccgatgatct
    13261 cgatgccgcg ctcaaaagcc ccgcgctcgc ccgctatcag aacatcttcc ttctggggta
    13321 ttcgctcggt ggcaacatga tgctccgcta tctcgccggt tctccggatc cgcgggtgcg
    13381 ggccgcggct gcggtttgct cgccgatcga cttgaagccc ggcgcccggg caatcgaccg
    13441 accgcgcggg ctcttctatc gaaggcacat cttgagcgcg ctgaaggaca tctacagtca
    13501 ggtcgctgcg cgccgcgagg ttcctctgcc actggagcag gcggtccgta tcgatacgat
    13561 cgagagctgg gacgagcgcg tgatttcgcc gcgtcacggt ttcgcgggcg ccgaggatta
    13621 ctgggcgcga accagtgctt gcaacatact cgaggagatc aaaacgccca cgctcttcat
    13681 cgcaagcgag cgtgacccga tggtgccgat cgacacggtg ctgccttggc ttcagactgc
    13741 gagccaatta cggtgggtcg tcaccccaaa ggggggtcac gtgggcttcc cgcagaaagt
    13801 gaacctgggt ctgggctcag ggggtaccgt cgaagaccaa atcctccaat ggatgcaggc
    13861 acccacatag caaaccgggc gaccctcgcg gccgccctct gctgttgctt cataggcggc
    13921 tgtcgacgtg aggagacccc tcgtgaaatg cctcagcgcc ccgcgtcttg tccggaaacg
    13981 cggcaggtcg aaccgccctt ggccaacgtc gcacccgagc acgaaaccct ggcctattgg
    14041 ctcgaacggg gtgaggctcg aggggggctc gacaagctgc tcctcagccc cgcgggagtg
    14101 cgtaggcaca acgccgcact tcgagaaccg cgcgacggcg agccgatcgg acaagcggac
    14161 ctaagggcgc ccgtcgatcg atcgaccctc gacgctgagg tcgaggaacg cttggcctat
    14221 ctgcggaagc ggatcgaagc ggctgagctc gtcaacgcca gcgggcacgc aatgagcgcg
    14281 gcggagttgg cgtattttgc gcttccggcg tccgcgccaa gcgtccaagc ggtcgaacag
    14341 tggaacctca tcgaagatct gacgcccctt cgttgcggcc cttacgatgg cggtttgcac
    14401 actcgccccg tcgacttgga cttcgatcgg aatcgatgca gcacgatgcg ccaaggtgaa
    14461 atcgttcagg tcttggcgcg ctggccaaat ggaatggctc tggcgcgaac gccctacgct
    14521 ctcggttggg tcgcgagtga cgccctctcc tcgcctctcg atcgcgagct gctcgaagac
    14581 cggctcgaac gaaaggagct cctgcctttc acgcggcgtg ccctgctgag cgaggcattt
    14641 tcgatgctcg gtgagccgta cggctggggc ggcaaagacg gaggctacga ttgctccagg
    14701 tttctcttgg aggtctttgg acgattcggg atcgacctgc cccgtcacag tgcgcggcag
    14761 gcggaagcag ggacgttcag tctcgacgtg tccgaggtcg aagacctcaa cgagaagaag
    14821 ctcttgatcg aagccgcggc acggcgaggc atcgtcctgt tgcacttccc gggacacatc
    14881 atgctttacc tcggcacgac ggaagccggc acgcccatgg cgatgcacgc cttttcggaa
    14941 tacctcacgc cgtgcaccga aaccgggctc gagaccgtga accgcgtcga ccgtgtggcg
    15001 gtgagcgacc tcagcctcgg cgcgggaagc tcccgcaatg actttctccg ccgaatcaca
    15061 cgggtcaccg tgctgggggg tgcgcccggt cccgcgctga gcgcggatgc cgagctccgc
    15121 gcgagcgcgc ccatctcatt tcccgaggac cgctgcgccg atactaagcg atttgcgatt
    15181 ttccacacgc cgcagaggcc gaacccgtct cagccgttgc gcgtgattgt cacgagcgac
    15241 gcggatcccg ggccggcatc gctcgtcctc ttcgccccgg acgggtcgca ggtcattcct
    15301 gattcgcatc ggctcgacgg accgccgcat gggcgctgga tcgaagtcga agcgcccaca
    15361 gcaggcgaat ggaccgcctt gttcgcagac ggcgaccggg tacacgcgtg cgagcgcatc
    15421 accgtccgca agcatccggc gcaaccggtt cgacgcgctt tccaggcgcc ggcatgggag
    15481 ccgcgccggc agtggggtca cgacaccgag aatctctacg ctgctttcgt cgagcagctg
    15541 ttccgtgaac ccgccggaga agatgtgacc tggtcgagct tgcaggagct gatcggatcg
    15601 cccgagagca acttgctcta cgatcacaat ctcccgggag aggacgcgct gctcgacctg
    15661 gaacccgatt gcgccgacct gccgtacttc ctgcgcgcct actttgcttg gaagctcggt
    15721 ttgcccttcg cctaccgcac atgcacccga ggcacgaaag catcccctcc agtctgcgag
    15781 cccaccgtgc tgagcaacct cgattcggtc cccgacagca acgaggcaga tgcgttccga
    15841 aggttcgtcc gccgcatggc cggaacggtg cactcgtcga gcccccgcac ggttccgagc
    15901 gacgatcaga cggacctcta ccccgttcga ctgaataggc gggccatgcc accaggaacc
    15961 gtctttgccg atccctacgg ccacgtgctc gtcgtcgccc gttggaagcc ccagggtgtg
    16021 accgactacg gggtcttgat gggcgccgac gcccaacctg acgggaccgt aggacggcga
    16081 cgcttctggc gcggctcctt cttgtttact cccaataccg agcttgtcgg cgccggattc
    16141 aaggcatggc gacccgttcg atacgacccg agcgttctgc cgggcgccgt gaccgggaac
    16201 gcggatggga acatcgaagg cgcgccggag ccagctgccg caccccagcc ctgggcgatc
    16261 ccgagcaacg aggagcttcg caaggcaggc ggaacccgcg catggtccga cgtgcagtac
    16321 gacggcagcg ccgacgattt ctacgcgacg attgaaggtc tcatcaaccc gcgcgcgctc
    16381 gatcccgtgc gaatgcaaat cagcctggtc gacgcgctcg aggagtctgt gcagcgccgc
    16441 ctgagctccg tgcagaacgg cgaggacttc atgaagagcc aggagtatgc accgatcgac
    16501 atgccccacg gtgccgcgct gttcctgacc agcggcccct gggaggatta ctccacacct
    16561 tctcgggaca tgcgcctgct gatcagcatc gacgcggtga tgacgttccc cgacagtgtc
    16621 ggcgcccatc ccgaacgctt tgggattccc gaaggcgagc aggcctccgc ggcgcagcaa
    16681 gtccgcgatg ccctccgagc agagcttgcg caacgctcat tcgaatacac acgctctgat
    16741 ggaagcgcct gggcgctcac gctgggggat ctcgtcgctc gggcaaagcc aatggagatg
    16801 gcgtacaacc cgaacgattg cccggagctt cgctgggggg cagcagaggg ctcacaggag
    16861 cacgcgactt gcaagagacg ggctccggca gcgcagcaat cgaggatgcg gacctacagg
    16921 aactggttcg caaagcggga gcgtccgggt tgagcagcgt cgagccctag cgccagacct
    16981 cgacgttgtc gtccacttcg tacccgtcgg tcgtccgtgg ctttggtccc cgggggtacc
    17041 ggggctcggg gtcgtcgata tctcgctgct tggcctcccc gcccttggga ggatttcccg
    17101 gcgattcttg aatcggcgtg ccgctcagcg ggagggactc gggcgtcggc aagggatccc
    17161 tcacgtttct gagctctggc tcgggcgtcc cctgagccgt ctcgatggtg agcggaggct
    17221 catacttgat ctcgatcacc gtggaggcag tctcggactg agcagtatcc tcgagcgggc
    17281 gcagggccag ggcgccgatt cggcgtttga tgcaatcgag cgcggcggtc gaaa
//
LOCUS       DASRZF010000313         2188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3337053, whole genome shotgun sequence.
ACCESSION   DASRZF010000313 DASRZF010000000
VERSION     DASRZF010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3337053"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="VFG22_13855"
     CDS             complement(<1..680)
                     /locus_tag="VFG22_13855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417376.1"
                     /translation="MRQRLENGRDEPRRVWCAWLVTLAWIAMSGLAPNMVRAEEVAEV
                     SQEALSKAQQELDEAQAKINEAQAKLDAATKALPEGEREPTAEEELDPVDPDDDSTDE
                     VEPVGPGLVAQDGEAKKLQWALNEDGSRYWRMALWLQVWTRAMQLNPGTTIGDDPATP
                     GETEGKEPAWYGDVAIRRAHVLMFGEIFPRVFLLMHFGINNQTFKRSNFKETFFFHDL
                     WVEFAAVK"
     gene            complement(716..>2188)
                     /locus_tag="VFG22_13860"
     CDS             complement(716..>2188)
                     /locus_tag="VFG22_13860"
                     /inference="COORDINATES: protein motif:HMM:TIGR03648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HET6417377.1"
                     /translation="NSARIVAVLCAVFVSFTYVAGQMRGVGVVFSRFLEVDINVGVLI
                     GMAIVFFYAVLGGMKGITYTQAAQYCVLIFAYLVPAIFISILLTDHLIPQIGFGGSLG
                     GEVGSRIANGETVTLLDKLNGLNTELGFAEYTSGKKSMLDVLCITGALMAGTAGLPHV
                     IVRFYTVPKVRDARLSAGWALFFIALLYTTAPAVAAFARANLIETVSGASYAQVEGEK
                     SIPQWFKTWEDTGLLAWADKNGDGKVQYVAGDALAGSKPEFTDVRGASGERMVSNANP
                     ETENELYVDRDIIVLANPEIARLPAWVIALVAAGGLAAALSTAAGLLLVVSTSVSHDL
                     LKRVFVPSITEKQELLAARVAAGVAVMIAGYFGINPPGFVAQVVAFAFGLACSSFFPA
                     IILGIFVKRANREGVISGMLVGITFTAAYIIYFKFLGGTPDQYWFGISPEGIGALGML
                     INFAVAFVVSRFTPPPPQEVQDMVEHIRVPVGSKAPTGMH"
BASE COUNT          447 a          699 c          634 g          408 t
ORIGIN      
        1 tccttcaccg ccgcaaactc cacccagaga tcgtgaaaga agaacgtttc cttgaagttc
       61 gaccgtttga aggtctggtt gttgataccg aagtgcatca acagaaacac gcgcgggaag
      121 atctcaccaa acatcagcac gtgcgcacgg cggattgcga cgtcaccgta ccaagcgggt
      181 tccttgcctt ccgtctcgcc gggggtagcc gggtcgtccc caatcgtcgt gccagggttc
      241 agttgcatcg cccgggtcca gacctgcaac cagagcgcca tgcgccaata ccgcgagccg
      301 tcttcgttca aggcccactg cagcttcttg gcctcgccgt cctgggcgac gagccccgga
      361 ccgacgggct cgacctcgtc ggtcgaatcg tcatccggat cgacgggatc gagctcttcc
      421 tcggccgtcg gttcacgctc gccctcaggg agcgctttcg tcgccgcatc tagcttggcc
      481 tgggcttcgt tgatcttggc ctgtgcttca tcgagctctt gctgagcctt gctcagcgcc
      541 tcctgcgaga cctctgcgac ctcttcggct ctcaccatgt tgggagccag tcccgacatt
      601 gcaatccacg ccagggtcac gagccaggcg caccacactc gtcgcggctc atcacggccg
      661 ttttccagtc tttgcctcat cacaaccctc ctatctgcgc tgcgtcagcc gttcattaat
      721 gcatgccggt tggtgccttc gagcccacgg gtacgcgaat gtgttcgacc atgtcctgta
      781 cctcttgggg aggagggggc gtgaatcggc tcaccacgaa ggcaactgcg aagttgatga
      841 gcatgccgag cgcgccgatg ccctcgggcg aaatcccgaa ccagtactga tccggagtgc
      901 cacccaggaa cttgaagtag atgatgtatg ccgccgtgaa cgtgatgccg accagcatgc
      961 cgctgatcac accttcgcga ttggctcgct tgacgaagat gccgaggatg atcgcgggga
     1021 agaacgaaga gcacgcgagc ccgaacgcga aggccacgac ttgtgcaacg aaaccaggtg
     1081 ggttgatgcc gaagtagccc gcgatcatca ccgcaactcc tgctgcgact cgggctgcca
     1141 gaagctcctg tttctcggtg atcgaaggca cgaacacgcg tttgagcagg tcgtgactga
     1201 ccgaagtgct caccacgaga agcaagcccg cagccgtcga aagagctgca gccaagccgc
     1261 ccgccgcgac gagcgcgatg acccaggctg gaagacgcgc aatttctgga tttgccagga
     1321 cgatgatgtc gcggtcgacg tagagctcgt tctcggtttc ggggttggcg ttcgacacca
     1381 tgcgttcgcc gctcgcaccc cgtacatcgg tgaactcggg cttactaccg gcgagcgcgt
     1441 cccccgccac gtactgaacc ttgccatcgc cgttcttgtc ggcccaagcg agtagtcccg
     1501 tgtcctccca agtcttgaac cattgcggta tcgacttctc gccctcgacc tgagcgtagc
     1561 tcgccccgct taccgtctcg atcaggttcg cacgcgcgaa cgcagccact gccggcgcgg
     1621 tggtgtagag gagcgcgatg aagaagagcg cccagccagc gctgagacgc gcatcccgca
     1681 ccttgggaac ggtgtagaaa cgcacgatga cgtgaggcag gccggccgtg cctgccatga
     1741 gcgctcccgt gatgcatagg acgtccagca tggacttctt gcccgacgtg tactcggcaa
     1801 aaccaagctc cgtattcagt ccgttgagct tgtccagcag agtgaccgtc tctccgtttg
     1861 cgatgcgaga gccgacctcg ccacccagcg agccgccgaa gcctatctgc ggaatcaaat
     1921 ggtccgtcag caggatgctg atgaagatag ccggcacgag atacgcgaaa atcagaacgc
     1981 aatactgagc agcctgagtg taggtgatgc ctttcatgcc accgagaacg gcgtagaaga
     2041 acacgatcgc catgccgatc agaacgccga cattgatgtc gacctccaag aagcggctga
     2101 agacgacgcc gacaccccgc atctgacccg caacgtaggt gaaggagacg aagaccgcgc
     2161 aaagcaccgc gacgatacgg gccgaatt
//
LOCUS       DASRZF010000314         4106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1964925, whole genome shotgun sequence.
ACCESSION   DASRZF010000314 DASRZF010000000
VERSION     DASRZF010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1964925"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /locus_tag="VFG22_13865"
     CDS             complement(<1..1161)
                     /locus_tag="VFG22_13865"
                     /inference="COORDINATES: protein motif:HMM:NF024628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HET6417378.1"
                     /translation="MQQLNSDLLAISFDSPASPTIHFKKGNRPDGWGFAWYPRDGVGA
                     SVLKDPHVEYDMAVATAVQRWDRFRSSVFVCHSGTHRGRSFTDTHPFVRSHAGRDWIF
                     AHNGDLGRNLSVSLPLDERLGFEPVGHTDSEHALCWLMGQVRAQGARSFADIGWQTIR
                     AWLHELNRLGTLNLVWSDGLHLVAYHDQNGFNELHHLRRLPPPTSEKLESDLLELDLS
                     NSLDENRTSTIISTRPLSGEPWSRFVPGEMRVISRGHVIETGETIPPPPPKRAFDTHS
                     SSQVDAEASQVGTRTQTSQVDVGMSQVGKGVGQRGAQMQRGGARLGGISPDPGSRART
                     MPAPGLPALGKKVSSHTYEVTHETIYRYETPVETSSHTVRLQPVRDLTQDVLE"
     gene            complement(1142..2140)
                     /locus_tag="VFG22_13870"
     CDS             complement(1142..2140)
                     /locus_tag="VFG22_13870"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HET6417379.1"
                     /translation="MISRVAGNCFWLNRYMERAGSMARLLRVNRAFVPGVDLPHLHRW
                     HPMIVVAGEEEQYLRHFGEDENGETVQEYLTWDSRNTTSVVESVRWARENARTAREIV
                     SSEMWETLNSLWHWLQGGPGRRLYRSDRDHFYQHIRAMGELFHGQADETMLNGEPLAF
                     MRLGRYLERAGQTARLMDVHYHTFPDEGAIDDPIRLAQWSALLACCSATEPFQKRSFG
                     TPTAREVVSFLLFEPDLPRSVSHCLRAARHSLVQVRGYGSNRIGMVSTALLEALLSHV
                     ALREGQTIIGADLHDELTYLVDSLTDLCASIEQEFFQSTALQAQILGEDHGAAAQQ"
     gene            complement(2137..3627)
                     /locus_tag="VFG22_13875"
     CDS             complement(2137..3627)
                     /locus_tag="VFG22_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HET6417380.1"
                     /translation="MQAETEQVVGLGADVTLLDGYAPLAKTYDELFRADGTPREDVAG
                     LVAELEQLGRVEFMTRQKLAEGALFRGGVTFSVYSDSRGQEKIFPFDLIPRVISAPTW
                     EVIERGLVQRVTALNMFLTDLYDGQRILKERRIPEAVVLGTSGYLKELHGIRPREGVF
                     VNIAGIDLIRGSKGEFLVLEDNLRTPSGVSYVLENRNVMKRVLPGVFRRSRIRTVESY
                     PAHLGDALMSVSPRDPDESRVVVLTPGPYNSAYFEHSFLARQMGCELVQGSDLFVHDL
                     HVYVKTTAGPQRVDVIYRRIDDDFLDPRVFREDSALGVPGLVGAYAAGNVALANAPGN
                     GVADNKAVYPFVPDMIRFYLGEEPVLGQVETYLCARPDDRQRVLENLKEMVVKAVDGC
                     GGYGMLFGPRASRNEIAEFAVKIRENPDGYIAQPVVELSTCPTWVDGEAAPRRVDLRP
                     FVLTGKRSWVLPGGLTRVALKAGSYVVNSSQGGGSKDTWVLEGPVS"
     gene            complement(3983..>4106)
                     /locus_tag="VFG22_13880"
     CDS             complement(3983..>4106)
                     /locus_tag="VFG22_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET6417381.1"
                     /translation="RAFRAAYREAYEAWRNGHRQTEFPLGTWWMVARFGVPVAP"
BASE COUNT          856 a         1298 c         1191 g          761 t
ORIGIN      
        1 ctcgagcacg tcttgcgtga ggtcccgaac cggctggagg cgaaccgtgt gggacgacgt
       61 ctcaacaggc gtctcgtacc ggtaaatcgt ctcatgcgta acctcgtacg tgtgcgagct
      121 gaccttcttc ccaagagcag gcaaccccgg agccggcatc gtccgcgcgc gtgaaccggg
      181 gtccggagag atccccccca accgagcccc accccgctgc atctgcgccc cgcgctgccc
      241 cacacccttc ccaacctggg acattcccac atcaacctgg gacgtctgcg tccgcgtccc
      301 aacctgggac gcttcagcat caacctggga agaactgtgc gtgtcgaatg cgcgtttggg
      361 tggtgggggc ggaatggtct cgcctgtctc gatcacatga ccacggctga ttacccgcat
      421 ctcgccgggc acaaaacgcg accagggttc tccagacaag ggtcgcgtgg aaatgatggt
      481 cgacgtccgg ttctcgtcga gcgagttcga gaggtcgagc tcgagcaagt ccgactcgag
      541 cttctcagag gttggtggtg gcaggcgccg aagatggtgt aactcattga atccgttctg
      601 atcgtgataa gcaaccaggt gcaacccgtc gctccagacc aagttgagcg tgccgagtcg
      661 gttgagctca tgcagccaag cacggatggt ttgccagcca atgtcagcaa agctacgcgc
      721 gccttgagcg cgaacctgtc ccatcaacca gcaaagcgca tgctccgaat ccgtatggcc
      781 gacaggctcg aatccaagtc gctcatcgag cggcagcgag acgctgagat tgcgcccgag
      841 gtctccgttg tgtgcgaaga tccagtcgcg tcccgcatgg ctgcgcacga atgggtgggt
      901 atcggtgaat gatcggccgc gatgggtgcc actgtgacaa acgaagacgc tagaccgaaa
      961 acggtcccag cgctggaccg ctgtggcaac ggccatgtcg tattcgacgt gcggatcctt
     1021 tagcaccgac gcgccaactc cgtccctcgg gtaccaggcg aaaccccagc cgtcgggacg
     1081 gttgcccttc ttgaagtgga tcgtcggcga agctgggctg tcgaaggata tcgcgagaag
     1141 atcactgttg agctgctgca ccgtggtcct ccccaagaat ctgagcctgc agcgcggtcg
     1201 actggaaaaa ctcctgctcg atcgaagcgc aaaggtcggt cagcgaatcc accaaatacg
     1261 taagctcatc gtgaaggtcc gctccgatga tggtttgacc ttcgcgcaaa gcaacatggc
     1321 tgagcagcgc ctccaaaaga gcggtcgaca ccatcccgat gcgattggag ccgtacccgc
     1381 gaacctgaac cagagaatgg cgtgccgcac gcaggcaatg ggacacggag cgcggtagat
     1441 ccggctcgaa tagcagaaag ctcacgacct cccgcgcggt cggcgtgccg aacgaacgct
     1501 tttgaaacgg ctcggtcgcg gaacagcacg cgagcaaagc ggaccattgc gcgaggcgaa
     1561 tggggtcatc gatggcgccc tcgtcgggaa acgtgtggta gtgcacgtcc atcagacgcg
     1621 cggtctgccc tgcgcgctcg aggtatcgcc cgagacgcat gaacgccaac ggttcgccgt
     1681 tgagcattgt ctcgtcagcc tgcccgtgaa agagttcgcc catggctcgg atgtgctgat
     1741 agaagtggtc tcggtccgag cgatagagcc gccgccccgg tccaccttga agccagtgcc
     1801 agagcgagtt gagcgtctcc cacatctctg agctcacgat ctcccgcgcc gtccgcgcgt
     1861 tctcgcgagc ccagcgcacc gactcaacca cggacgtcgt gttccggctg tcccaggtta
     1921 gatattcctg aaccgtctcg ccgttctcat cctcgccaaa gtgacggaga tattgctctt
     1981 cctcgccggc gacgacgatc atcgggtgcc agcgatggag atgcggcaag tctaccccgg
     2041 gcacgaaagc gcgattcacc cgcaaaaggc gcgccatgct tcccgcacgt tccatgtagc
     2101 ggttgagcca gaagcaattg cctgcaaccc gggaaatcac gaaacgggcc cttccaatac
     2161 ccaggtgtcc ttggacccgc ccccttggct cgagttgacg acgtacgacc cggccttcag
     2221 cgcaacccgg gttaatcctc ctggcaaaac ccagctccgt ttcccggtca acacgaacgg
     2281 ccggagatca acccgtcgag gcgctgcctc cccatcgacc caggttgggc aagtcgagag
     2341 ctcgacgacc ggttgcgcga tgtagccgtc ggggttttca cgaatcttta ccgcgaactc
     2401 tgcgatttcg tttcggctcg cccgtggccc gaacagcatg ccgtagcctc cgcagccatc
     2461 gacggctttg acgaccatct ctttgaggtt ctcgagcacg cgttggcgat cgtccggccg
     2521 agcgcagagg taggtctcga cctgtccaag aacgggctcc tccccgaggt agaaacggat
     2581 catgtcggga acgaacgggt acacggcctt gttgtccgcg accccatttc ctggcgcatt
     2641 cgccaaggcg acgttcccgg ccgcgtaagc gccaaccaaa cctgggacgc ctagagcgct
     2701 gtcctcccga aacacgcgcg ggtcgaggaa atcgtcgtcg atcctccgat agatgacgtc
     2761 gactcgctga ggacctgccg tggtcttcac gtagacgtgc aggtcatgaa caaacaggtc
     2821 gctcccctgg acgagctcgc agcccatttg gcgggccaaa aaactgtgct cgaagtacgc
     2881 tgagttgtaa gggcctggcg tcagcacaac gactcggctt tcgtccgggt cgcgcgggga
     2941 gacactcatg agcgcgtcac ccaggtgggc gggataactc tcgacggtgc ggatgcgact
     3001 tctccgaaat acgccgggaa gtacgcgttt catcacgttg cggttttcca aaacgtaact
     3061 gacacccgac ggcgttcgga gattgtcctc gagcactaag aactcgccct tcgaacccct
     3121 gatcaggtcg atcccggcaa tattgacgaa aacgccctcg cggggtcgga ttccgtgcag
     3181 ctccttcaag tagccactgg tgcccaaaac gaccgcttca ggaatccgcc tttccttcag
     3241 gatgcgctgc ccgtcgtaga ggtcggtcag aaacatgttc agagccgtaa cgcgctgaac
     3301 cagcccccgc tcaatcactt cccaggttgg agcggagata acgcgcggga tgaggtcgaa
     3361 gggaaagatt ttttcttgtc cacgagaatc agaatagaca gaaaatgtga ccccaccgcg
     3421 aaagagggcg ccctctgcga gtttttggcg agtcatgaac tcgacacgac cgagttgttc
     3481 tagctcggcc accagaccgg cgacgtcctc acgaggagtt ccatcggctc taaatagctc
     3541 gtcgtaggtc ttcgccagcg gcgcatagcc atccagcaat gtgacgtccg caccgagacc
     3601 aactacctgc tctgtctcag cttgcatgat gatgagacaa gatgggcgca gccagtaccg
     3661 cgatccagct caacttcagt gacaagccta cttcttacct ccgcgaaaca atgccgaaat
     3721 catcggaaac tttcccaggt tcgtctccgc atgtcgcgtg ctgtccactc tcgtgacccc
     3781 ctgcaggatc gacgttgctg ggaccgggac aactgaacct atcgaacatc agctgggctg
     3841 ccccccgcgg cgaacgatcg ccactcctgc acccgcatcg atatgggctc tgcagagggc
     3901 acgcagtcgc tagcgaagcg acccacgcat cagagcgtgt ggaatagatg aggcatctca
     3961 ggagacactt cgccgagaac gtctatggcg cgactggaac gccgaagcgg gcgaccatcc
     4021 accatgtccc caaggggaac tccgtttgcc tatggccgtt tcgccatgcc tcgtatgctt
     4081 ctcggtacgc cgctcgaaag gctcga
//
LOCUS       DASRZF010000315         5099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1620251, whole genome shotgun sequence.
ACCESSION   DASRZF010000315 DASRZF010000000
VERSION     DASRZF010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 5099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5099
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1620251"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VFG22_13885"
     CDS             <1..275
                     /locus_tag="VFG22_13885"
                     /inference="COORDINATES: protein motif:HMM:NF013067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HET6417382.1"
                     /translation="AVLGHVDAIVFTAGVSEHSADIRERACEGLEGLGVQLDEARNRA
                     GAKGVRSIHRRGAPLAVLVVPTNEELEIAEQTLECIERDLTEVGGE"
     gene            391..2766
                     /locus_tag="VFG22_13890"
     CDS             391..2766
                     /locus_tag="VFG22_13890"
                     /inference="COORDINATES: protein motif:HMM:NF003619.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HET6417383.1"
                     /translation="MSNISERALSTEDIDNIDAYWRACNYLAAGMIYLQANPLLREPL
                     RPEHIKSRLLGHWGASPGLSFMYVHLNRLIRMHDLNAIFLAGPGHGAPGVLAPVYLEG
                     TYSERYPEKSEDVDGMRRFFKQFSFPGGIGSHCTPETPGSIHEGGELGYSLSHAFGAA
                     FDNPDLLVAVAIGDGESETGPLATAWHSNKFLSPVTDGAVLPILHLNGYKINNPTILA
                     RISHEELEQLFRGYGWTPHFVEGDDPADMHLRMAATLERCLVEIRDIQGEARAAGKAT
                     RARWPMIVLRTPKGWTGPAEVDGHRVEGFWRAHQVPLTGVREDPKRLAQLESWLKSYR
                     PDELFDQTGRLVPELKALAPIGDRRMSANPVANGGLLRKALRMPDFREYGLKVERPAQ
                     TRAENTRPLGRLLRDVMRRNPDRFRVFGPDENTSNKLDAIYEVSKKLWLADYLPEDED
                     GGELARDGRVLEMLSEHTLEGWLEGYVLTGRHGFFSTYEAFVHVIDSMYNQHAKWLAI
                     CNEIPWRAPISSLNLLITSTVWRQDHNGFTHQDPGFLDVVVNKNPEVTRIYLPPDVNT
                     LLSVSDHCLRSMNNINVIVSDKQKHQQYMDMDDAVNHCTKGIGTWDWASNDQGSEPDV
                     VMVGCGDVPTQEALAATAILREHFPALKIRFINVVDLFKMQPEGEHPHGLSDRDFDGL
                     FTKDKPVIFNFHGYPWLIHRLAYRRTNHANLHVRGYKEKGNINTPLELAMDNEIDRFS
                     LAIDVINRVPELQVAGAHVKDTLRDRQIECRAYAHRHGVDVPEVSDWRWPY"
     gene            3110..4132
                     /locus_tag="VFG22_13895"
     CDS             3110..4132
                     /locus_tag="VFG22_13895"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HET6417384.1"
                     /translation="MRASKLLLCGLLLIFACGDNDDPGPSSVADVLYVGRFDDSDPGA
                     VRLSWSGSGFVVRFRGTGARATFRGGRYFTVVVDGDVRPDPLAVSDIVGATYDFASNL
                     PDGEHTIEVYRRTEASLGPTVVGGVEVDGEPLAVPRSTRQLEIVGDSWSTGDGVDGSV
                     EVCDSSAQNHFLTWGAIAARAVDAELSTVAWGDKGIVREPLSALYDRVVGTEAGSTGT
                     IRPADAVFVSLGANDFIEGELPNGFVPAYASLLEQIRLQHPDAIIACIWPELPDASET
                     SLARSAIDAAIAERAAAGDARVTGADLELDELVDFACDNGHPGVETHALMAENTIAFL
                     RAELGW"
     gene            4431..4811
                     /locus_tag="VFG22_13900"
     CDS             4431..4811
                     /locus_tag="VFG22_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417385.1"
                     /translation="MDRRIKFFHWAPRTLCILAILFVSMFALDAFEGTHTLWEQLGHF
                     FMHLIPSFVLLIVFFVATKWELIGGILFTLIGVALSPPLFILNYRMNESIGMSLGVIM
                     AITGPFIVVGILFVISSRLKKNHQ"
BASE COUNT          945 a         1581 c         1599 g          974 t
ORIGIN      
        1 tggcagtcct aggccacgtc gatgcgatcg tcttcactgc gggcgtgagc gaacactcgg
       61 cggacatccg cgagcgcgcc tgcgaggggc tggaggggct cggcgtgcaa ctcgatgaag
      121 cgcgcaaccg ggccggtgcg aagggcgtcc gatcgatcca ccgtcgaggc gctccgctgg
      181 cggtgctggt cgtgccaacc aacgaggagc tggagatcgc cgagcaaacc ctagagtgca
      241 ttgaaagaga tcttactgag gttggcggcg agtgaacgcg ccgtgccggc ttgatcggtc
      301 gtcgacctcg cgtatcagat gctgtcggcg ttaaggcatg ccgaccacgg acccctgggc
      361 taggcaggtc ggagacggag agaaatgcag atgagcaaca tttcagaacg cgcgctttcc
      421 acagaggaca tcgacaacat cgatgcgtac tggcgggcat gcaactacct cgccgccggc
      481 atgatctatc ttcaggcgaa cccgctcctc cgcgagccgc tcaggcccga gcacatcaag
      541 agtcggctgc tcggccactg gggcgcaagt cccggtctct cgttcatgta cgtgcacctg
      601 aaccggctca ttcggatgca cgatctgaat gccatcttcc tggcgggacc cggccacggc
      661 gcgcccggcg tgctcgcgcc cgtgtatctg gagggcacct actcggagcg ctacccggag
      721 aagagcgagg atgtcgacgg catgcgacgg ttcttcaagc agttctcttt ccccgggggc
      781 attggcagcc actgcacacc cgagacgccc ggctccatcc atgagggcgg tgagctcggc
      841 tatagcctct cgcacgcatt tggcgcggcg ttcgacaatc cggatctgct ggtggcggtc
      901 gcgatcggag acggcgagtc cgagaccggt ccgctggcca cggcctggca ctcgaacaag
      961 tttctgagtc ccgtcaccga cggtgctgtg ctgccgatcc ttcatttgaa cggatacaag
     1021 atcaacaacc ccacgatcct cgcgcgcatc tcacacgaag agctcgagca actcttccgc
     1081 ggctatggtt ggacgccgca tttcgtggaa ggcgacgacc cggccgacat gcacctgcgg
     1141 atggcggcga cgctggaacg ttgtctcgtt gagatccgcg acattcaagg cgaggcgcgg
     1201 gcagccggaa aggcgacgcg cgcgcgctgg ccgatgattg tgctgcgtac gccgaagggc
     1261 tggaccggtc ccgccgaggt ggacggccat cgggtcgagg gattctggcg cgcgcatcag
     1321 gtgcctctca ccggcgtacg ggaggacccg aaacgcctcg cccagctcga gagctggctg
     1381 aagagctaca gaccggatga gctcttcgac cagaccgggc gcctcgtacc ggagcttaag
     1441 gcccttgctc ctatcggcga ccgccggatg agcgccaacc ccgttgcgaa cggaggattg
     1501 ctccgcaaag cactgcgcat gccggatttc cgcgagtacg ggttgaaggt ggagcgtccc
     1561 gcgcagacac gcgcggagaa cacccgaccg ctggggcgac tgctgcgcga tgtgatgcgg
     1621 cgcaacccag accgctttcg cgtgttcggt ccggacgaga acacctcgaa caagctggac
     1681 gcgatctatg aagtgagcaa gaagctttgg ctcgcagatt acctgccgga ggacgaagat
     1741 ggcggtgagc ttgcccggga cggccgcgtg ctggaaatgc tctccgagca tacgctggag
     1801 ggctggctgg agggctatgt gctcaccggc cgccacggct tcttctcgac ttacgaggcc
     1861 ttcgtgcacg tcatcgactc gatgtacaac cagcatgcga aatggctcgc catctgcaac
     1921 gagatcccct ggcgtgcgcc gatctcttcg ctgaatctac tgatcacctc gacggtgtgg
     1981 cgccaggatc acaacggctt cacccatcag gatcctggct tcttggacgt ggtcgtaaac
     2041 aagaaccccg aggtgactcg catctatctg ccgccggacg tgaacacgct gttgtcggtg
     2101 tcagaccact gcctcaggag catgaacaac atcaacgtca tcgtttccga caaacagaag
     2161 catcaacagt acatggatat ggacgacgcg gtgaaccact gcacgaaagg tatcgggacc
     2221 tgggactggg ccagcaacga ccaggggtca gagcccgatg tggtcatggt cggctgcggc
     2281 gatgtcccca cgcaggaggc cctcgccgcc accgcgatcc ttcgcgagca tttcccggcg
     2341 ctcaaaattc gcttcatcaa cgtggtcgat ctgttcaaga tgcaacccga gggcgagcat
     2401 ccgcacggcc tctctgatcg cgacttcgac ggcctgttca ccaaagacaa gccggtgata
     2461 ttcaacttcc acggttatcc atggctgatc caccggcttg cctacaggcg cacgaatcat
     2521 gcgaatctac acgtgcgtgg ctataaggag aagggcaaca tcaacacgcc gctggagctc
     2581 gccatggaca acgagatcga ccgcttcagc ctggcgatcg acgtcatcaa ccgcgtgcca
     2641 gagcttcagg tggccggggc gcacgtgaaa gacacgcttc gtgaccgaca gatcgaatgt
     2701 cgcgcgtacg ctcaccggca cggcgtcgac gtgcccgagg taagcgattg gcgctggccg
     2761 tattgacgaa caccgttgtt gggcacggcg ttgtggtggc ctgtcgttcc tcagaacgtc
     2821 gtgcgtcgcg gggtgtgagc cacgatgctt gggaaggcga ccagccgggc agagctcgga
     2881 cccttgtcag tttgtggcac cctcgagtca gtggattgca tcgcgaactg ccggaacctg
     2941 aacggttgcg gaaaattcgg aagctaggtg agggacgaga cggcggcacg ggccgcagag
     3001 ttcgtcgagg agcgggtgaa gcgcttccgg caggggacca aaccggccta gactggcact
     3061 ttctcggatt gtcctcggcg aactcggttt cgatggaata aggtgcggca tgcgcgcttc
     3121 caagctgttg ttatgtggcc tgctgctcat tttcgcctgt ggggacaacg acgaccccgg
     3181 tccatcgtcc gtggccgacg tgctctacgt gggtcgcttc gacgattccg atccgggcgc
     3241 ggttcgtctt tcgtggtccg ggagcggctt cgtggttcgc ttccgcggga ccggcgcgcg
     3301 ggccacattt cgcggtggac gctacttcac ggtggtcgtc gacggtgacg tccggcccga
     3361 tccgctcgcg gtgtccgata tcgtcggcgc gacctacgac ttcgcctcga atctcccgga
     3421 cggcgaacac accatcgagg tctaccgccg caccgaagcc tctcttggac cgaccgtggt
     3481 cggcggggtg gaggtcgacg gggagccgct cgcggtgcct cggtcaaccc gccagctgga
     3541 gatcgtgggc gactcgtgga gcacgggaga cggcgtggac ggttccgtcg aggtctgcga
     3601 ctcgagcgcc caaaaccact tcttgacgtg gggcgccatc gcggcccggg cggtcgacgc
     3661 cgagctcagc accgtggcct ggggggacaa gggcatagtc cgtgagcccc tgtcagccct
     3721 ctacgaccga gtggtcggga ccgaggccgg cagcaccggg actatccgcc cggccgacgc
     3781 ggtgttcgtc tctcttggcg ccaacgactt catcgaaggc gagctgccca atgggttcgt
     3841 gcccgcctac gcctcgcttc tggagcagat tcgccttcag caccccgacg cgatcatcgc
     3901 ttgcatttgg cccgagctcc ccgacgcgtc ggagacgtcg ctcgcccgct cggcgatcga
     3961 cgcggccatc gccgagcgcg ccgctgccgg cgacgcccgg gtcaccgggg cggacctcga
     4021 gctcgatgag ctcgtcgact ttgcctgcga caacggccac cccggtgtcg agacgcacgc
     4081 cttgatggcc gagaacacga tcgcgttcct gagggccgag ctcggttggt agctccggca
     4141 ttccagagct ttatcgccag cctgaggact gcttaggttt cgagcgcgac atctctacgc
     4201 tggcctccgg cacggtccca cgtcgaagct catcatgctc tcatccgaag cccgtcccac
     4261 ccttcgatga aagcggaaaa ttggttccaa cttcgcatcg ttggggtcac cacgaagaat
     4321 cccctgcact tcgcggggat ttctttctgt gggtcggggc gtcgcgccga cagggccttt
     4381 gttttcgcta cgctcttctt ttcgatagtc tgtggtaagc ctgcagaact ttggataggc
     4441 gtatcaaatt cttccattgg gctccgcgaa cactttgcat tctcgccatt ctcttcgtga
     4501 gcatgtttgc cctcgacgcc tttgaaggca ctcacactct ttgggaacag cttgggcatt
     4561 ttttcatgca cctcatccct tcattcgtcc ttttgatcgt cttcttcgtg gcgaccaaat
     4621 gggaactgat cgggggtatt ctcttcacct tgataggtgt tgcgttgagc ccgcccttat
     4681 tcatcctcaa ctacaggatg aacgagtcga tagggatgag tcttggggta atcatggcga
     4741 ttaccggacc gttcatagtc gtaggcatac tgttcgtcat cagtagcagg ctgaaaaaga
     4801 accatcagta gcaagctgat agccgctcaa gaaacgcctt tttcccccgg ccgaaagtag
     4861 acagagaagg cgtctatcga cgacgccaac gaaacgtgat ctcgggctgc aacgcccgtg
     4921 aacggttcgc gagagagacc gagggcctac ttcgtgcgcg tggcgatcgg gtcgccacat
     4981 tcgctgcagc tcaccagttg gctgccggcg aacagctcgt cacacagagt gtaggcttcc
     5041 tctgtgcacg tgggatcggg gtcgtcacac ttgccgcagc tcacgagcga gccgccgcc
//
LOCUS       DASRZF010000316         4333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_5053802, whole genome shotgun sequence.
ACCESSION   DASRZF010000316 DASRZF010000000
VERSION     DASRZF010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 4333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4333
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5053802"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            363..1517
                     /locus_tag="VFG22_13905"
     CDS             363..1517
                     /locus_tag="VFG22_13905"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HET6417386.1"
                     /translation="MTATTRIENGTAEGTTLFVSFELGESTWQLAFTTGRGQKARQKT
                     ITGRKLDTVIAELERARRRFDLPAGCRVASCYEAGREGFWLHRWLVSRGIESHVVDAA
                     SIEVNRRLRRAKTDRLDASRLLSLLVRFAEGECSVWSVVHVPSPEDEDRRHLHRELLT
                     AKRDRTRNANRIQGLLANQGVRVDWRKPLPAQLSMLRLWDGSSLPAGLCERLGHEWDR
                     LELLNQQIEGLERTRREQIREAADPASEKAKRLVALKAIGSNSAWLYVMEFFGWRKFR
                     NRREVGALAGLTPTPYQSGESSREQGIAKSGNRHVRAMAIEIAWGWLRFQPKSQLARW
                     YEERFGRGSSRLRRIGIVALARKILIALWRYLETGVVPEGAELKLAATVR"
     gene            1898..2395
                     /locus_tag="VFG22_13910"
     CDS             1898..2395
                     /locus_tag="VFG22_13910"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6417387.1"
                     /translation="MAKKRDEERPAVSLREITSENLRMVLALRVSEEQKQAYPRSNAY
                     SIAEGHYPRDSDPVWMRAIYADEAPVGFIMTSEARENGEYFLWRLMIDQNHQGKGYGS
                     AAVGLLVARIEASGNGRYLVTDHLEEDGNAGSFYTRHGFRYTGEVLSEGELVMRRDLN
                     GRDVR"
     gene            2661..2873
                     /locus_tag="VFG22_13915"
     CDS             2661..2873
                     /locus_tag="VFG22_13915"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HET6417388.1"
                     /translation="MKFRILVEQDEDGAFVASVPSLPGCVSQGATRQEAVASVREAIE
                     GYLESLRDRGEPIPPSIHEEVIDVAV"
     gene            3257..3865
                     /locus_tag="VFG22_13920"
     CDS             3257..3865
                     /locus_tag="VFG22_13920"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HET6417389.1"
                     /translation="MRLLDFWFEFGSTYSYPAAMRIEALAKSEGIAIAWRPFLLGPIF
                     HDQGWDDSPFNIYPAKGRYMWRDLERVCAQQGLPVRRPSQFPRNGLLAARLAFRFPSE
                     PWLPEFVRRVYVANFAEDRDIADSQVLESVLQALAQPVALLAEAQSVEAKAGLRSQTE
                     RAVSMGIFGAPSFTVGGELFWGNDRLEAALSYAKSEAAKYAV"
     gene            3992..>4333
                     /locus_tag="VFG22_13925"
     CDS             3992..>4333
                     /locus_tag="VFG22_13925"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET6417390.1"
                     /translation="MRVALLSDIHGNRIALDAVIEDARCFGIDVFCAVGDLAAIGPEP
                     VSVLEALAELENITIVRGNTDRYIVTGEGPPPTLEQAQADPRLVDLHARVRASFAWTR
                     GFVTSAGWFEWL"
BASE COUNT          794 a         1323 c         1429 g          787 t
ORIGIN      
        1 gcacgagctc gacggcctcg cgcaggttgt ctcgcgcctc atcgagcgtg gcgccctggg
       61 tgtttgcacc gggaagctct tccccgaaag cgatatatcc ctcgggaaca cgcctaaaca
      121 cggccgtgaa tgccatctcc atcgccattt ctccgagcct cccagctggc taacaagccg
      181 ctgcagctga cggccgcaag ctctgcccgc atggggccat tatcgcccgt caggcatggt
      241 agcatccccg gcggccgcag ctgagcggcc atccgttgga tcctatgcgc atgcaaggga
      301 gagagagcgg gtcgctgcgg ttaagctcct tcgaagtcgt cacacaacgg aggagcacga
      361 cgatgaccgc aacgacccgc attgagaatg gtactgcaga gggcacgact ctctttgttt
      421 cgttcgagct cggggaatcg acctggcagc tggccttcac gacgggccga gggcagaagg
      481 cccgtcagaa gacgatcacg ggccggaagc tggacacagt catcgcggag ctcgagcgcg
      541 cgcggcggcg cttcgatctg ccggcgggct gccgggtcgc gagctgctac gaggctggcc
      601 gggaaggctt ctggctgcat cgctggctgg tgtcgcgtgg gatcgagagc cacgtagtcg
      661 acgcggcgag catcgaagtc aatcggagac tgcggcgtgc gaagacggat cggctggatg
      721 cctcgcggct gctctcattg ctggttcgtt tcgcggaagg agagtgcagc gtctggagcg
      781 tggtccacgt gccgagcccg gaggacgagg accggcggca ccttcaccgc gagctgctga
      841 cggcgaagcg agatcgcaca cggaacgcga atcggatcca gggcctgctc gccaaccagg
      901 gcgtccgggt ggactggcgc aagccgctac cggcgcagct ctccatgctc cggctctggg
      961 atggctcgtc gcttcccgcg ggcctttgcg agcggctcgg ccacgagtgg gatcggctcg
     1021 agctcctcaa ccaacaaatc gagggactcg agcgcacgcg ccgcgagcag atccgcgaag
     1081 cggccgaccc cgccagcgag aaggcgaagc gcctggtcgc cctcaaggcc atcggctcga
     1141 acagcgcctg gctctacgtg atggagttct ttggctggcg caagttccgc aaccggcgcg
     1201 aggtgggagc gctggcgggt ctgacgccga ctccctacca gagcggggag tcgagtcggg
     1261 agcaaggaat cgcgaagtcc ggcaaccggc acgtgcgggc gatggcgatc gagatcgcct
     1321 ggggctggct ccgcttccag cccaagagcc agctcgcccg gtggtacgag gagcgcttcg
     1381 gccgcggcag cagccggctg cgccggatcg ggatcgtggc gctggcgcgc aagatcttga
     1441 tcgcgctctg gcgatacctc gagaccgggg ttgtgccgga aggcgcggag ctcaagctgg
     1501 ccgccacggt gcggtaggga aagaaaggac gcgaccagag gatcgagcga gttggtgcgg
     1561 aaggcccgtt attgccgccg ggttcgccac gcgaaccgac cgttggatgg ggtctccgcc
     1621 acggcacttc ccagtcgcct gcgggcgaac acaggatgcg gtgaccggcc tccacaagcg
     1681 ccggcacgga tagagggtcg tccggggctc gaagccctga cgcaagaaga agtcgccagc
     1741 tcggccggta ttctgcttca tcggcacgga tcgcccatgt agaatcgagt ctcaacggag
     1801 cttgacagca acggccacat agagggcggc atgagttgcg acgaaagctc gatattcccg
     1861 aagaccgcta acaattcgtg gaagaggcca agctgtgatg gccaagaagc gtgatgaaga
     1921 gaggccggcg gtgagcctcc gcgagattac aagcgagaac ctcaggatgg tgctcgcgct
     1981 cagagtctcg gaagagcaga aacaggcgta tccgcgctcc aatgcttact cgatagctga
     2041 aggacactat ccacgtgata gtgatcctgt gtggatgcgc gcaatctatg ccgacgaagc
     2101 tcctgttggg ttcatcatga cttccgaggc tcgtgaaaac ggagagtact tcctttggcg
     2161 gttgatgatt gatcagaatc atcagggcaa aggatacggc tcggcggctg taggtctgct
     2221 tgttgcccga attgaagcca gtggaaacgg caggtacctt gtaaccgatc atctcgagga
     2281 agatggcaat gcagggagct tctacactcg ccatgggttt cgctacacag gagaagttct
     2341 gagcgaaggc gagctagtaa tgcggagaga cctcaacggc cgcgatgttc ggtagcatcc
     2401 ccagccaggt gagtgccaat cgcggacatc cgcccaacaa agcgttgaac gcggacgtgg
     2461 gaacagctcg cttcaatcga gccaggtagc cttctggcat cacacttcac tggctcggcg
     2521 agtaccggcg gcgctgttcc cacgctggtt aacgccgatc cgttgggcag cgccaagaag
     2581 acgccgggcg ggcgcgctcg gcgacgtttg cagccggcgt tatccttgcg gcgagtcatc
     2641 gattctcgga ggccggcctg atgaagtttc gaatcctcgt ggagcaagac gaagatgggg
     2701 ccttcgtggc ctccgtgccc tcgcttcccg gatgcgtctc acagggggcc acccgccaag
     2761 aagccgtcgc cagcgtccgc gaagcgatcg aggggtacct tgagagcctt cgggatcgag
     2821 gcgagcccat ccccccgtca attcacgagg aagtcatcga cgtcgcagta tgagcacgct
     2881 tccgcggatc tcgggtcgcg acgcggtggc tgcattccgg cgtcttggat acgagatcga
     2941 ccgccagaag ggcagccata ttgttctccg ccacccggcg cctcccttcc tcggctcacc
     3001 attcccgatc atcgcgagct tgcaaaggga acgctccggg ccctgattcg cgaggcggga
     3061 atcacagtgg aagagttcgt ctctctcgtc cgttagcagc gtgcgctgtc caacaagggc
     3121 ttgcagctgt cgggagcccg gtgtgagctt tcggtccgtt tgggtgtctg gcatcctgct
     3181 caggggtttc ggcggaactc ggacggcccg ggctctcgca gctgaagccc gatccgttag
     3241 accgcgcgag aggcaaatga ggctgctcga cttctggttc gagttcggaa gcacctactc
     3301 gtatccggca gcgatgcgga tcgaggcact cgccaagtct gaaggtatcg cgatcgcttg
     3361 gcgccccttt cttctcgggc cgatctttca tgaccagggg tgggacgact ctcccttcaa
     3421 catctacccc gcgaagggtc gctatatgtg gcgcgacctc gagcgtgtct gcgcacagca
     3481 gggtcttccg gttcgccgcc cctcgcagtt tccgcgcaac ggcctccttg cggcacggtt
     3541 ggcgtttcgc tttccgtccg agccttggct tcccgagttc gtccgccgcg tctacgtggc
     3601 caacttcgcg gaggaccggg acatcgccga ctcccaggtg cttgaatccg tcctccaggc
     3661 gctggcccag cccgtggcgc tcctcgccga ggcgcagtct gtcgaggcca aggcagggct
     3721 tcgctcacag actgagcggg ccgtatccat gggcatcttt ggggccccaa gcttcactgt
     3781 gggtggtgaa ctcttctggg ggaacgaccg tctcgaggcg gccctctcct acgcgaagtc
     3841 ggaggcagcg aaatacgcgg tctaacaagg cgatgaagct gacggccatc gctcgggccg
     3901 actttggtag cgtcctggcg gcgatgctgg ttgcctcgct gctgtcggtc agcggctggc
     3961 agcttgtcgc tgatccgtaa gacagtggga catgcgagtc gccttgttgt cggacatcca
     4021 cggaaaccga atcgccctag atgccgtcat cgaggacgcg aggtgcttcg gaatcgatgt
     4081 cttctgcgcc gttggggatc tcgcggcgat cggaccagaa cccgtgtcgg ttctcgaagc
     4141 gctagccgag ctcgagaaca ttacaatcgt tcgtggcaac actgaccgct atatcgtgac
     4201 tggcgaaggc ccgcccccaa ccctcgagca agcccaagcc gatccgaggc tcgtggacct
     4261 ccatgctcgc gtcagggcat cctttgcttg gactcgtggc ttcgtcacgt cggcaggatg
     4321 gttcgagtgg ctc
//
LOCUS       DASRZF010000317         5880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2308670, whole genome shotgun sequence.
ACCESSION   DASRZF010000317 DASRZF010000000
VERSION     DASRZF010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 5880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5880
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2308670"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2824
                     /locus_tag="VFG22_13930"
     CDS             <1..2824
                     /locus_tag="VFG22_13930"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HET6417391.1"
                     /translation="NIDADPNSMNQLVMYENLVFSDAYPTPGELSDEDLVPGYFKDAP
                     LRDESSFDSVRTVTDGTRAARIGRDEMGVPHIFGEARGDVLFGTGYATATDRMFAIDV
                     LRHVGRGRMSDFLGPAGGNYSSDRGLARFGGYDEPEIQAQIDQLAPRFGEDGVQAQDD
                     VDNFVRGINRYIDDILSGAEGAERVPIEYAALGLELLPFTARDVLAVAALVQSTFATG
                     GGSEHRQVELLQGLAALFPGDTAAACETWRDIRQANDPERPNTTEVPFETQSPATIDE
                     SACPLDEGFAAAYPGAVLFDEGSLEELELLTIEDCVSPEDAGPGDIECPALGEDVVDD
                     EVPAASAMMIFRHETGESTWLKDGIPYRLGEPSRDALQTGRARARATVEGIRLALNGL
                     SFPDAMSNAILVNGEETEDGHPIAVFGPQTGYFSPQMLVEFSQQGGGIHSRGMAFAGL
                     PYVIIGRGIDHAWSATSSGDDITDVRVLRLCEPGGGAPTRASTSYLYDGVCTPMLERT
                     DEWTAETNLTTAGTPNQRVRRNILRAPDYGPVFATATVNGAPVALAVQRSTFFGEVDS
                     VRPFLAATRNDMVDPDAFFDVFNSLTGTFNWFYVDANNIAYFNSGLLPIRAPGIHPDL
                     PQWGTGEFDWQQTGTGRINSEFSFDNFVPLLGHPHEANPEKGYFANWNNAQAPGFYAN
                     DGQTGYGRLYRSELLSRRLDAFREQPGAPGLTRANMVQIMIDAGTTDLRGQEILPRVF
                     EVLGDVSDLSESEQEIVLLMKDWVANGPNGLGAMRRDRNGPGLDTSSLEYEDHAAVAF
                     MDRWWNPMIDALLPQIKEVEDAGVMIGGRHDSPGAIGSAFQGGYYGYVARVLEMALGE
                     SGAPYNRIACAGTGELVDCRAALVASLQQTMSDLGADMAQWDPTLEQDDAINHTALGL
                     ADPPNIHWQNRPTWQQVVQPTEDLLQ"
     gene            complement(2847..3041)
                     /locus_tag="VFG22_13935"
     CDS             complement(2847..3041)
                     /locus_tag="VFG22_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417392.1"
                     /translation="MKRTIQRITLLVVAVLCLGTSDCYTGTYVGYGYGPGYGPSYYND
                     VFYSPPIDRYGYGYGFYGRP"
     gene            complement(3038..3946)
                     /locus_tag="VFG22_13940"
     CDS             complement(3038..3946)
                     /locus_tag="VFG22_13940"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6417393.1"
                     /translation="MTQSAKLVRIESLLTAVALLCSASILGCSSSPSLPPLVEDQDAP
                     ALPGKFVWHNLITNDGEAARTFYGELLGWKFDVKKDGRYSVITYRGHNLGGILDASKD
                     ENPPKSGRWLSAISVADLDESLAALSEAGGKQVDAPINVSGVGRVVTIEDADGALLHL
                     LASEHGDPPDIETKTHTWLWHELLANHADRAVAFYEAAFGYQTEQLPDRPSGDYRVLT
                     SAGAPRAGVIDNPFESTRSIWIPYLRVDDPAALAERASALGGTVVLAPNPSLRDGNLA
                     LILDPTGAPIALQKWSRKEEAEGASL"
     gene            4073..4837
                     /locus_tag="VFG22_13945"
     CDS             4073..4837
                     /locus_tag="VFG22_13945"
                     /inference="COORDINATES: protein motif:HMM:NF006100.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HET6417394.1"
                     /translation="MSVRFETRNHVAIITLDRPEARNAINGVMSTGIEAALDQYESDD
                     ALWAAVLTGNGPVFCAGADLKEIASGNAAATATARGGFGGIVHRERSKPLIAALNGPA
                     LAGGCEIALACDMIVASDASFFGLPEVKRSLIAVAGALFRLPRAIGMTQAMEVILTGE
                     PLLAARAYELGLVNRLVPVDRVVEEALGLAEKIASNAPLAVQASRALAARAFSADEAT
                     LWTEGFEAFQKIMQSEDAKEGPLAFIQKRAPSWKAR"
     gene            complement(4853..5392)
                     /locus_tag="VFG22_13950"
     CDS             complement(4853..5392)
                     /locus_tag="VFG22_13950"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HET6417395.1"
                     /translation="MHTVGDPFPEFSLQAVVSTEPGKEFEEITNRSDPDKWQVIFFWP
                     MDFTFVCPTEIADFGRKNAAFEERNAQVLGGSTDTHYVHLAWRNDHPDLKKLPFPMLA
                     DTKRELSTALGILHPDAGVCLRATYIVDPEGIIRFVSVNDLSVGRNVDEVLRVLDALQ
                     TGGLTACGWTPGEATLDAA"
     gene            complement(5416..5841)
                     /locus_tag="VFG22_13955"
     CDS             complement(5416..5841)
                     /locus_tag="VFG22_13955"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HET6417396.1"
                     /translation="MLSDDTTTLETHGVKPSAQRVAIAGYVLHTKDHPSADEVWEKVK
                     RAFPMVSRATVYNTLNLFVEKGLIRHLVLTEGRLVFDPNVDKHHHFIDEDSGKIYDLP
                     WDTLKVSRVEQLEGFDVRDYQVLVRGKKRKEKRQTKSKY"
BASE COUNT         1076 a         1773 c         1935 g         1096 t
ORIGIN      
        1 gaacatcgac gcagacccca actccatgaa ccagctcgtc atgtacgaga acctggtgtt
       61 cagtgacgcg tatcccacgc ccggggagct gagcgacgag gacctcgtcc caggatactt
      121 caaggatgcg ccgctgcgcg acgagagctc gttcgattcg gttcgaacgg tgacggacgg
      181 cacccgcgct gcgcgcatcg gccgcgatga gatgggcgtt ccgcacatct tcggcgaggc
      241 ccggggcgat gtgctcttcg gcaccgggta cgcaacggcg accgaccgga tgtttgccat
      301 cgacgttctg cgacacgtgg gtcggggacg gatgagcgac ttcctcggtc ctgccggcgg
      361 gaactactcc tccgatcggg ggctcgcccg gtttggtggt tacgacgagc cggagattca
      421 ggctcagatc gaccaactcg cgccgcgatt cggcgaggac ggcgttcagg cacaagacga
      481 cgtcgacaat ttcgtgcgcg gaatcaatcg gtacatcgat gacatcctga gcggtgcgga
      541 aggtgcggag cgcgtaccca tcgagtacgc ggcgctcggt ttggagctgc ttccgtttac
      601 ggcgcgcgat gtgctcgccg tcgcggcctt ggtgcagtcc acgtttgcga ccggaggcgg
      661 tagcgagcat cggcaggtgg agctcctgca agggcttgct gcgctttttc cgggggatac
      721 tgcggcagcc tgtgagacct ggcgcgacat ccgtcaggcc aacgacccgg agcgtcccaa
      781 tacgaccgag gtccccttcg agacgcagtc acccgccacg atcgacgaat cggcatgccc
      841 gctcgacgaa ggattcgccg cggcgtatcc cggagccgtg ctcttcgatg agggtagcct
      901 cgaggagctc gagctgctca caatcgagga ttgcgtttcg cccgaggacg cgggccctgg
      961 ggacatcgag tgtccggcgc tcggagagga cgtcgtcgac gacgaggtgc ccgcagcgag
     1021 cgcgatgatg atcttcaggc acgaaacggg agagtccacc tggctgaagg acgggattcc
     1081 gtatcgcctg ggcgaacctt cccgtgatgc gctgcaaacc ggtcgagccc gcgcgcgcgc
     1141 gaccgtcgag gggatccggc tcgcgctcaa cgggctgagc tttccggatg cgatgtcgaa
     1201 cgcaattctg gtgaacgggg aagagactga agacgggcat ccgatcgccg tattcgggcc
     1261 gcaaaccgga tacttctcgc cccagatgct cgtcgagttc tcgcagcaag gcggcgggat
     1321 ccacagccgc gggatggcgt ttgctgggct tccctatgtc atcatcggtc gcggcatcga
     1381 ccacgcctgg agtgcgacct cttcgggcga cgacattacg gacgttcgtg tgctccggct
     1441 ctgcgagccc ggtggcggcg cgcccacgcg cgcttcgacg agctatctct acgacggagt
     1501 ctgtacgccg atgctcgagc gaacggacga gtggacggcg gagaccaatc tcacgacggc
     1561 ggggacgccc aaccaacggg tcaggcgcaa cattcttcgc gcgcccgact atggtccggt
     1621 ctttgccacg gcgacggtca acggagcccc ggtggccctc gcggtgcagc gatccacgtt
     1681 cttcggtgaa gtcgattcgg tgcgaccgtt tctcgcggcc acacggaacg acatggtcga
     1741 ccccgacgcc ttcttcgatg tgttcaacag cctcaccggc acgttcaact ggttctacgt
     1801 cgatgcgaac aacatcgcct atttcaactc gggcctcttg ccgatccgcg cgccaggcat
     1861 tcatccagac ttgccgcagt gggggacggg cgaattcgat tggcagcaga ctggcactgg
     1921 gcgaatcaat tcggagttca gcttcgacaa cttcgtgccg ctgctcggcc acccccacga
     1981 ggcgaaccca gagaagggct acttcgcgaa ttggaacaac gcgcaggccc caggcttcta
     2041 cgcgaacgac ggacaaaccg gctatggccg cctgtaccgt tccgagctct tgtctcgacg
     2101 gctggatgcg tttcgcgagc agccaggggc cccgggcctc acgcgcgcga acatggttca
     2161 gatcatgatc gatgcgggta cgacggatct tcgggggcag gagatactgc cccgcgtctt
     2221 cgaggtactc ggggacgtga gcgacctcag cgaatccgag caggagatcg tattgctgat
     2281 gaaggactgg gtcgcgaatg ggccaaacgg cctcggagcc atgcgacgcg acaggaacgg
     2341 gcctgggcta gacacctcga gcctcgagta cgaggaccat gcggcggtcg cgttcatgga
     2401 tcgctggtgg aacccgatga tcgacgcctt gctgccgcag atcaaagagg tcgaggacgc
     2461 aggcgtcatg atcggcggcc ggcacgattc gcccggggcc atcggctccg cgtttcaggg
     2521 tggctactac ggttacgtgg cgcgcgttct cgagatggcg ctcggagaga gcggggcgcc
     2581 gtacaatcga atcgcctgcg cgggaaccgg cgagctcgtg gattgccgcg cggcgctggt
     2641 cgcgagcctt caacagacga tgtcagacct cggcgccgac atggcgcaat gggatcccac
     2701 gctcgagcag gacgacgcga tcaaccacac ggcgcttggg ctggcagacc cacccaacat
     2761 ccactggcag aatcggccga cctggcagca ggtcgttcag cccaccgaag acctgttgca
     2821 gtgacgtcga ggacctggat cagcgctcag ggtcgtccgt agaacccgta tccgtagccg
     2881 tagcggtcga tcggcgggga atagaagacg tcgttgtagt agctcggtcc gtatccaggc
     2941 ccgtagccat aaccgacata ggtcccggtg tagcagtcgg aggtacccag gcagaggacg
     3001 gccacgacca gcagcgtgat gcgttggatg gtgcgtttca caggctcgct ccttctgctt
     3061 cttctttgcg cgaccatttt tgcagggcga tcggtgcgcc ggttggatcg aggatcaagg
     3121 cgaggttgcc gtcgcggaga ctcggattcg gagcgagcac gacggtgccc ccaagcgcgg
     3181 acgcgcgttc ggcaagagct gccgggtcat cgactcgaag gtatggaatc catatcgagc
     3241 gggtagactc gaacgggttg tcgatgactc ctgcacgcgg tgcacctgca ctggtgagaa
     3301 cgcgatagtc tccgctcggc cggtccggga gttgttcggt ctggtaccca aacgccgctt
     3361 cgtagaacgc gaccgcgcgg tccgcgtggt ttgcgaggag ctcgtgccag agccaagtgt
     3421 gagtcttcgt ttcgatgtcg ggcgggtcac cgtgctcgga agcgaggaga tggagcagag
     3481 cgccatccgc gtcctcgatg gtcaccacac ggcccacccc ggacacgttg atcggagcgt
     3541 cgacctgctt gccgcctgcc tcgctgagcg cggccaggct ctcgtcgagg tccgcgaccg
     3601 agatcgcgct cagccagcgt cccgacttcg gtgggttctc gtccttgctc gcgtcgagga
     3661 tgccgccgag gttgtgcccc cggtaggtga tgacgctgta tcgaccatcc tttttgacgt
     3721 cgaacttcca gccgaggagc tctccgtaga acgttcgcgc agcttcaccg tcgttggtga
     3781 tgaggttgtg ccagacgaac ttgccgggaa gcgccggtgc gtcctgatct tcgaccaggg
     3841 gaggaaggct ggggctgctg ctgcagccga ggatcgaagc cgagcagagc agggcgacgg
     3901 ccgtcaagag gctttcaata cggacgagct tggctgattg ggtcatgcgc ctccttggcg
     3961 gggtcttgaa acgagctgga ccgtacacca tctagctctt tgcgtcgaaa gcaggtatcg
     4021 acgcatgcgc aagatatgcg cacgacctcg aacactgcga aggagcccct gcatgagcgt
     4081 ccgatttgag acaaggaacc acgtcgccat catcaccctc gaccgacccg aggcgcggaa
     4141 cgccatcaac ggagtgatgt ccaccggcat cgaggcggcg ctcgaccagt acgaatccga
     4201 cgacgcgctt tgggcggccg tgctgaccgg aaacggccca gtcttttgtg ctggcgccga
     4261 cctcaaggag attgcatcgg gcaatgcggc cgcaacggcg acggcgcggg gcggcttcgg
     4321 gggcatcgtt catcgcgagc gcagcaaacc cctaatcgca gccctcaacg ggccggctct
     4381 tgccggtggc tgcgagatcg cgctcgcctg tgacatgatc gtcgcctccg acgcgtcgtt
     4441 cttcggcctg cccgaggtga aacgatccct cattgcggtg gcaggagcgc tctttcgtct
     4501 gccgcgcgcc atcggcatga cgcaggccat ggaagtgatc ctcacgggcg agcccttgct
     4561 ggcggcgcgc gcgtacgagc tcggtctcgt caaccggctc gttccggtgg accgcgtcgt
     4621 cgaggaggcg ctagggcttg ccgaaaagat cgcctcgaat gcgccgcttg ctgtacaggc
     4681 gagccgggcg ctggccgcgc gagcgttctc cgcagacgag gcaacgctct ggaccgaagg
     4741 gttcgaggcg tttcagaaga tcatgcagag cgaggacgcc aaggaaggtc cgctcgcgtt
     4801 cattcaaaag cgcgcgccat cttggaaagc gcgatgagcg ctcagctggg tgttaagccg
     4861 cgtccaaagt cgcttcgccg ggagtccagc cacaagcggt gagcccgcct gtttgcaggg
     4921 catcgagcac tcggagcact tcgtcgacat ttctaccgac ggagaggtcg ttgacgctca
     4981 cgaagcggat gattccttcg gggtcgacga tgtacgtcgc tctcagacag acgccggcgt
     5041 ccgggtgcag aatgccaagg gccgtcgaaa gctcgcgctt ggtatcagct agcatgggga
     5101 aggggagctt cttgagatcg ggatggtcgt ttcgccaggc cagatggacg tagtgcgtat
     5161 cggtgctgcc ccccaagacc tgggcgttgc gttcttcgaa tgccgcgttc tttcggccga
     5221 agtcggcgat ctccgtcggg caaacgaacg tgaagtccat cggccagaag aatatgacct
     5281 gccatttgtc ggggtcagag cggttcgtaa tctcttcgaa ctccttccct ggctcggtgc
     5341 tcaccaccgc ttggagtgag aactctggga atggatcacc tacagtatgc atgtttcctc
     5401 cttgtgaaac ggcaactagt atttggattt ggtctggcgc ttttccttgc gcttctttcc
     5461 gcggaccagg acttggtagt cgcggacgtc aaagccttcg agctgctcga ctcgtgacac
     5521 cttcaaggtg tcccaaggca agtcgtaaat cttgccgctg tcttcatcga tgaagtggtg
     5581 gtgcttgtcg acgtttgggt cgaagacgag gcgaccttcg gtgaggacca gatgacggat
     5641 caggcccttc tccacgaaga ggttgagcgt gttgtacacg gtcgcgcgag acaccatggg
     5701 gaacgcgcgc ttcacctttt cccagacctc atcggccgag ggatgatcct tcgtgtgcag
     5761 aacgtagccg gcgatggcga cgcgctgcgc cgacggcttg acgccgtgcg tttcgagggt
     5821 cgtcgtgtcg tcgctcagca taaaactaga tatagtctaa agttagacga tgtcaacgaa
//
LOCUS       DASRZF010000318         9847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_590301, whole genome shotgun sequence.
ACCESSION   DASRZF010000318 DASRZF010000000
VERSION     DASRZF010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 9847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9847
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590301"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VFG22_13960"
     CDS             <1..207
                     /locus_tag="VFG22_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HET6417397.1"
                     /translation="GRYASLKGIMQAKKKPIDKMSLSDLGVDASKALNYTKFELPPAR
                     TGETTFVADVTELVDKLHNTAKVI"
     gene            238..1200
                     /locus_tag="VFG22_13965"
     CDS             238..1200
                     /locus_tag="VFG22_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HET6417398.1"
                     /translation="MANVLVVAELLEGKARKTTLCAVTAAKQIAEGTGGSFDILAIGE
                     GAAAAAAELAGYGAGKVLKAEIAGGYVCEKYAATVAETGKAYDVLVACASAYGKDLLP
                     RAAAKLDAGVASDISAVHTDGGKLSYTRPMYAGNVSGTVEILSEKQVVTVRQSEFAAA
                     EASGGASPSEDVALQTDPAAGKVEFLGVEVVKSERPDLTEADIVVSGGRALKSSENFT
                     NVIEPLVDALGAAMGASRAACDAGYVSPELQVGQTGKVVAPKLYVAIGISGAIQHLAG
                     MKGSKTIVAINKDKEAPIAQVADYFLVADLFDAVPALTEAVKKL"
     gene            1504..1821
                     /locus_tag="VFG22_13970"
     CDS             1504..1821
                     /locus_tag="VFG22_13970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417399.1"
                     /translation="MCLGEFSPKNRFCVRDGAELTDGRSEGSFAQGMICPTCRRGYPF
                     DSRFCPEDADDLVPYGLFGAASATRPSLRLDANKICPECGVRHAPAHSFCGNDGTELV
                     VVN"
     gene            2001..3704
                     /locus_tag="VFG22_13975"
     CDS             2001..3704
                     /locus_tag="VFG22_13975"
                     /inference="COORDINATES: protein motif:HMM:NF025597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYF domain-containing protein"
                     /protein_id="HET6417400.1"
                     /translation="MKIVCDSCGAKYSIADEKVAGKIFKIRCKKCSSVLEVRGDQTGA
                     HPFEETSPAQEQGGDSTWYIVVDGEQKGPLPPIELSQLFAKGSVGLDSYVWKEGFDDW
                     KVAGDVPELTQIFGGGAGRAKAAPSEIDDTSSFGTGGDLFGDGSGREPTSEIETDGNM
                     FAGFDESPTPTGSQDSPFAAAAGNDASGGLGMGLGGAGMGASAPQRMTGQRNENSVLF
                     SLSNLQQLASRGGSAAESAPVASRASVAASAQGAPQAAGEGSGLIDIRSLAKSTTPGT
                     RAAPSQAVDDLLSLGTSGAALGTPLLGPAPLETSDGNRWLWIGGAAIAVIAIVGAGIG
                     VGMALGGSDDDALEITAEPAQQPSQPVPGSSAIAAAAQAPAEPPGQALAVAEPIEAAT
                     IEEDPIEEEAAPSIETPEPRKRTSTSSQRRQRPTRSSGTRSEAEASPPPKTRSGNNLD
                     ALMDEVVGGSSPSKPKQATKSSSPSDSSLPDAPSRDDVKTALQGVSGAVKGCKQDEGG
                     TATVDVTFSGSSGRATGAKVASGPFKGTPVGSCIESAVKRARVPRFKQSTFKVTFPYR
                     I"
     gene            complement(3708..5177)
                     /locus_tag="VFG22_13980"
     CDS             complement(3708..5177)
                     /locus_tag="VFG22_13980"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HET6417401.1"
                     /translation="MKISLSEGTWDNSNVDIVALAIPSGKTNFNRALGRIEVVTGKGS
                     IKPLASDERFEGKPTQTLKVAAGGKAKSRWLLLVGIGDSKDAQKIAWNLGHALATTAG
                     AQKSGAVELPEVTSESVRAASQGLVAGAYRYTEYKSDKAADSTLGSAVILGARKGAPG
                     LNAAVRAGKAIAESVNFARDLVNCPPNDLNPVTLARAASSESKKLGLSCRVWNKTRIE
                     KEGMNLLLAVNKGSAIEPRVVHVVYKPARAKKKVVFVGKGLTFDAGGLCIKPAASMVD
                     MKCDMAGAASTLGIVFAAARLKLPVEVHALVGSTENMTGAKAYRPGDVYKSLEGKTVE
                     VINTDAEGRLVLADVLTWAVRKLKPDYMIDHATLTGACLVALGPWRAALYTDDDALAS
                     KYLDAAQGEDEAFWRMPLDSDLRSTLKSSIADIKHIGGRFGGSITAALFLKEFVGETT
                     WMHLDIAGPAFVERAHGRLPKGGTGFGVATGVRFLESLG"
     gene            5382..5597
                     /locus_tag="VFG22_13985"
     CDS             5382..5597
                     /locus_tag="VFG22_13985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417402.1"
                     /translation="MLEENKVKIAYTVVERNKDGRKFWVRVGAAFVNRDGSLNVRLDA
                     MPVNGELQIRDYQPREARDPADSERAA"
     gene            5594..5899
                     /locus_tag="VFG22_13990"
     CDS             5594..5899
                     /locus_tag="VFG22_13990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HET6417403.1"
                     /translation="MIRAILLLALVLSVLPAGALANAGAASPDQGPPSVEQDQRININ
                     EAGPEELVRLPGVGPARAQAIIAEREKRRFRRIEDILRVPGIGRKTFDRLRQSIRVR"
     gene            5940..6428
                     /locus_tag="VFG22_13995"
     CDS             5940..6428
                     /locus_tag="VFG22_13995"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6417404.1"
                     /translation="MAQTIEVRDADASDRIALIDFHRSLYQEHRDKVVAAEDLPLIEY
                     RDYDQVLHDDLDALLRDRSGYVLLAESEGAALGYITGRVRTEPDRVLPRRGVIEDWFV
                     VPEARGSGVGAKLLHALEKRFAATGCEVIESATWAGNTGARHAHDALGFREIRVMYRK
                     RV"
     gene            complement(6930..7502)
                     /locus_tag="VFG22_14000"
     CDS             complement(6930..7502)
                     /locus_tag="VFG22_14000"
                     /inference="COORDINATES: protein motif:HMM:NF017939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-(ms[2]io[6]A)-hydroxylase"
                     /protein_id="HET6417405.1"
                     /translation="MPRLRLATSPSWIEVVLEDFDSFLVDHAACERKASATALKLVSH
                     YSDRTLLVRELIPFAQEELDHYAQVMKIILERGLETRADEKDPYVGALMQLIKRGPEQ
                     YFLDRLLVLGVVEARGCERFGLVADALEPGALKDFYVEITRSEARHHGLFVRLAKEYF
                     PTPRVQARLDELLDAEAKIVSELPHRPAVH"
     gene            complement(7513..8151)
                     /gene="hisG"
                     /locus_tag="VFG22_14005"
     CDS             complement(7513..8151)
                     /gene="hisG"
                     /locus_tag="VFG22_14005"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HET6417406.1"
                     /translation="MPRRNRDNIVIAVPKGRVLEQLSARFEKAGISTEALTAKSRKLV
                     REDKEAGLSYLLLKPDDVPTYVEYGAADLGIVGRDVLMERNYDVFAPVDLDIGKCQMM
                     VCGRPKEPIEGKGERPLRVATKFPHIAGQYFQRKGTPVELIFCQGSVELAPLTGLADV
                     IVDLVETGETLRQNGLVPLEKIAEISSVVIANRAALKLRRDAIQPILDALRA"
     gene            complement(8144..9409)
                     /gene="murA"
                     /locus_tag="VFG22_14010"
     CDS             complement(8144..9409)
                     /gene="murA"
                     /locus_tag="VFG22_14010"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET6417407.1"
                     /translation="MDSIRIIGGNRLVGSVPISGAKNAALPIMTAALLADGEHVFRNV
                     PNLRDVHTMTSLLERLGAKAEYVDGRVHVSLSGPIEPTAPYDLVKQMRASVLVLGPLV
                     ARCGKAMVSLPGGCAIGARPIDQHLKGLSAMGAKVELHHGYVEVNVPGGRLRGSDIAL
                     DFPTHTGTENLMSAAVLARGRTTLTNCAREPEIEELARVLNKMGARIEGAGTSVISIE
                     GVDSLHPIDHAIIPDRIEAGTFMVAAAATSGDLLLEGIGFEPLEAVIAKLRESGTVIE
                     REGDGIRVVGVRPIRPTDVITQPHPGFPTDMQAQFMMLMCLAEGTSRITETVFENRFM
                     HVSELNRMGARIQVNGRTATVQGVDRLEGAEVMATDLRASASLVVAGLVAKGTTYVRR
                     VYHLDRGYEQIEKKLAPLGANIERVAGNA"
     gene            complement(9448..>9847)
                     /locus_tag="VFG22_14015"
     CDS             complement(9448..>9847)
                     /locus_tag="VFG22_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417408.1"
                     /translation="GDPQGALLAWRSLSGGLAAARFLYSGEDPRREKADAQIARLMAK
                     DRAPGIDAGSSEEQLAADHRRLLSERVSPSPWWGSLLLLGMTGWIVALLILVRRGFDS
                     SGRFRWAGARAPLCGAVIGFVSFALGLLFA"
BASE COUNT         1877 a         3065 c         3154 g         1751 t
ORIGIN      
        1 ggccgatatg catctctcaa gggcatcatg caggccaaga agaagcccat cgataagatg
       61 tcgctcagcg acctcggcgt cgatgcgagc aaggcgctca actacaccaa gttcgagctg
      121 ccgccggccc gcacgggcga aacgacgttc gtcgcggacg tcacggagct cgtcgacaag
      181 ctccacaaca ccgccaaggt catctaagcc tcgagaaacg gaaaggaagc atcagacatg
      241 gctaatgttc tcgttgttgc ggagttgctt gagggcaagg cacgcaaaac cactctttgc
      301 gcggtgacgg ccgcaaaaca gatcgcggaa ggaaccggcg gctcattcga cattctcgcc
      361 attggagagg gtgcagctgc ggcagccgca gagctcgcag gctacggcgc aggcaaggtg
      421 ctgaaggcgg aaatcgctgg cggttacgta tgcgaaaaat acgcggccac cgtcgcagag
      481 accggcaagg catatgacgt gctggtcgcc tgtgcgagcg cgtatggcaa ggacctcctc
      541 ccgcgcgcgg ccgcgaagct cgatgccggt gttgcttccg atatctcggc ggtgcacacg
      601 gatggcggaa agctctcgta cacgcgaccg atgtacgcgg gcaacgtgtc tggcacggtc
      661 gagattctga gcgagaagca ggtagtcact gtgcgtcagt cggagttcgc cgccgcggaa
      721 gcctcaggcg gagcgagccc ctccgaggat gttgcgcttc agaccgatcc ggcagcgggg
      781 aaggtggagt tcctgggcgt cgaggtcgtg aagagcgagc gtccggatct cacggaggcc
      841 gacatcgtcg tctccggcgg ccgtgcactc aagagctcgg agaacttcac caacgtgatc
      901 gagccgctag tcgacgcgct cggggccgcc atgggtgcgt cccgtgccgc ttgcgatgcg
      961 ggatacgtct cccccgagct ccaggtcggt cagaccggca aggtggtcgc gccaaagctg
     1021 tatgtcgcca ttggtatctc cggagcgatt cagcacttgg ccggcatgaa gggcagcaag
     1081 acgatcgtgg cgatcaacaa agacaaggaa gccccgatcg cccaggttgc ggactacttc
     1141 ctcgttgcgg acctcttcga cgccgttccg gccttgacgg aagccgtgaa aaagctctga
     1201 gccccggggc cagataatcg agaaatccag ctcgagaagc ccgggagtct agcctcccgg
     1261 gcttttttgt ggctttgagc ggcggcgatc ggtcacaatt cagagatggc gtggtctctc
     1321 gttgttccgg cgacactcgg cgcggtgctt gcggtgctcg cagtgctggg gctgcagcgc
     1381 agacgtgcaa aaaaagcgac tgaccgttca ggttccagcc gcccgcccct cgctgcggag
     1441 aagggacctt cggagggagc caagcgaggc aagcccgagc ccgccccagt gaagagctgc
     1501 ccggtgtgcc ttggggagtt ctcgccgaag aatcggttct gcgtccgaga tggcgccgaa
     1561 ttgaccgatg gacgctccga gggctcgttt gcgcaaggca tgatctgccc gacgtgccgg
     1621 cgcggctatc ccttcgattc gcgcttctgc cccgaagatg cagatgatct ggtgccgtac
     1681 gggctcttcg gggctgccag cgcgacccgc ccttccctcc ggctcgatgc caacaagatc
     1741 tgccccgaat gtggggttcg ccacgccccc gctcactcct tctgcggcaa cgacgggacc
     1801 gagctggtgg tggtcaatta gaaaagaccc aaggattcga cgagatgagg gtccaagcac
     1861 gcgagcagaa ccttcgtgga cttcttgctt ggcatgggac aggaccgcta tgatcgcggt
     1921 cctaaagggg aatcttcgat ccggctgata tcgagtcatt tgtcgactgg atttgggcag
     1981 gagggagacg ggggcaacag atgaagatcg tttgcgatag ctgcggggcc aaatactcga
     2041 tcgccgacga aaaggtcgcg ggaaaaatct tcaagatccg gtgcaagaag tgctcatcgg
     2101 tactcgaagt tcgcggtgac cagaccggcg cacatccttt cgaagagacg agcccagcgc
     2161 aggagcaggg cggtgattcg acctggtaca tcgtcgtcga cggagagcag aaaggacctt
     2221 tgcctccgat cgagctgagt cagttgtttg ccaagggcag cgtcggcttg gactcgtacg
     2281 tttggaagga aggcttcgac gattggaagg tcgcgggcga cgttccagag ctgactcaaa
     2341 tatttggcgg cggtgcagga cgagcgaagg cagcgccgag cgaaatcgac gacacttctt
     2401 cgtttggcac cggcggcgat ctgttcggtg acggcagcgg acgcgaaccg accagcgaga
     2461 tcgaaaccga cggaaacatg tttgccgggt ttgatgaaag ccccaccccg accggatcgc
     2521 aggattcgcc cttcgctgca gcggcaggaa acgacgcaag cggcggattg gggatgggtc
     2581 tcggaggagc cggcatgggc gcatcggcgc cacagcgcat gaccggccaa cgcaacgaga
     2641 actccgtcct cttctcgctc agcaatcttc agcagctcgc gtcacgcggt ggctcagcag
     2701 cagagtcggc cccagtggcg agccgcgcat cggtggccgc aagcgcacaa ggcgctccac
     2761 aagccgcggg tgagggctcc ggtctgatcg acattcggtc gctcgcgaaa tcgaccacac
     2821 ctggcaccag ggcagcgcct tcgcaggcgg tcgacgatct gctttcactc ggaaccagcg
     2881 gagcggcgct cggcacacca ttgcttggtc cggccccgct cgagacatcc gatggaaatc
     2941 gctggctctg gatcggcggc gccgcgatcg cggtgatcgc catcgtgggt gcaggcattg
     3001 gcgtgggcat ggcgctcgga ggatcagacg acgacgcgct cgagatcacc gccgagccgg
     3061 cgcagcaacc cagccaaccg gtcccaggat cgagcgcgat cgcggccgca gcgcaggcgc
     3121 ctgcggaacc gccaggtcag gccctcgccg tcgcagagcc catcgaagcg gcgacgatcg
     3181 aagaggaccc catcgaagaa gaggctgccc cctcgatcga gacaccggag ccacgcaaac
     3241 gaacctcgac cagctcgcaa cgccgccagc gcccgactcg gagctccggg actcgcagcg
     3301 aggctgaggc gtcacctccg ccgaagacgc gttcgggcaa caacctcgat gcactgatgg
     3361 acgaagtcgt cggcggttcg tcaccgtcga agcccaagca ggctacgaag tcatcgagcc
     3421 cgagcgactc gagcctccca gacgcacctt cgcgtgacga cgtgaagacc gcccttcaag
     3481 gcgtgagcgg tgccgtcaaa ggctgcaagc aagacgaggg cggaacggcc accgtcgacg
     3541 tgacattcag cgggtcctcg ggccgcgcca ccggcgcgaa ggtggcctcc ggtccgttca
     3601 agggcacccc ggttggctcg tgcatcgaat ccgcggtcaa acgcgcacgc gttccgcgat
     3661 tcaagcaatc gaccttcaag gtgaccttcc cctaccggat ctgacgatca gccaaggctc
     3721 tcgaggaaac ggacgccggt tgcaacgccg aagccggtgc ctcccttggg aagccgacca
     3781 tgcgcgcgct cgacgaatgc cgggcccgcg atgtcgaggt gcatccaggt ggtttcgccg
     3841 acgaactcct tgaggaaaag cgccgccgtg atcgagccgc cgaagcggcc accgatgtgc
     3901 ttgatatccg cgatcgagct cttgagcgtg gagcgaagat cggaatcgag aggcatacgc
     3961 caaaacgctt catcctcccc ttgcgcagcg tcgaggtact tcgatgcaag cgcatcgtca
     4021 tcggtgtaaa gcgcggctcg ccagggaccg agggcgacca ggcacgcacc tgtgagtgtc
     4081 gcgtgatcga tcatgtagtc tggcttgagc ttgcgcacgg cccaggtcag cacgtcggcg
     4141 agcaccaagc gcccctcggc gtcggtgttg atgacctcga ctgtcttgcc ctccaaagat
     4201 ttgtacacgt cgccgggccg gtaggccttg gcgccggtca tgttctcggt ggagccgacc
     4261 agcgcgtgca cctcgacggg tagcttgagc cgcgcagcag caaacacgat gccgagcgtg
     4321 gaggcggcgc ctgccatgtc gcacttcatg tccaccatgg aggccgcggg cttgatgcac
     4381 aggccgcccg catcgaaggt gaggccctta ccgacgaata cgactttctt cttcgcacgt
     4441 gcgggcttgt agaccacgtg aaccactctc ggctcgatgg cgcttccctt gttcaccgcg
     4501 agcaagaggt tcatcccttc cttctcgatg cgggtcttgt tccagacgcg acaagacagc
     4561 ccgagctttt tggattcgct cgacgcggcg cgtgccaagg tgaccggatt caaatcgttc
     4621 gggggacagt tcaccaaatc tcgggcgaag ttgacgctct cggcgatggc cttgcctgct
     4681 cgcacagcgg cattgaggcc cggagcgccc ttgcgcgcac cgagaatcac ggcggacccg
     4741 agcgtcgagt ccgcggcctt gtcgctcttg tactcggtgt agcgatacgc tcccgcgacc
     4801 aggccttggg aagccgcgcg gaccgattcc gaggtgacct ctggcagctc gaccgcgccg
     4861 ctcttttgcg cacccgcggt ggtcgcaagt gcatgaccta gattccaggc gatcttctgg
     4921 gcatctttcg aatcgccgat gcccacgagg agcagccaac gagacttggc cttacctccg
     4981 gccgcgactt tgagcgtctg ggtcggcttg ccctcgaatc gttcgtccga agcaagtggc
     5041 ttgatgctgc ccttgccggt cacgacctcg atgcgcccga gggctcgatt gaagttcgtc
     5101 ttcccggatg gaatggcaag cgcaacgatg tcgacgttgg aattatccca ggtgccctca
     5161 gacagtgaaa tcttcatctg actccttatc aacttggtcg gccggatact gacaggaact
     5221 tctgcagcca acaaacgaac gcgtcgcaaa gatggcgtgg cggtcgctgt tgccctcggc
     5281 cgcttcgccg attcgagtgc cgaagcgagt ggggatcact gggagcagcc ctggcattgc
     5341 gcgtgcatgt ctccgagctt gcattccaac taggaggaca gatgctcgaa gaaaacaagg
     5401 tgaagatcgc ttacacggtg gtggagcgaa acaaggatgg tcggaagttt tgggtgcgcg
     5461 tcggcgcggc tttcgtcaat cgtgacggca gcctcaacgt ccggctcgac gcaatgccgg
     5521 tcaacggtga gctgcagatt cgagattacc agccgaggga ggcccgtgac cctgcggaca
     5581 gtgagcgggc tgcatgatta gagcgattct cctgctagcg ctggtcctga gcgttctccc
     5641 cgcgggtgcg ctcgcgaacg ccggagctgc gtcgccggac caagggcccc cgagtgtcga
     5701 gcaggaccag cggatcaaca tcaacgaggc cgggcccgag gagctcgttc ggctcccggg
     5761 cgtcggcccg gcccgcgcgc aggctatcat cgcggagcgc gaaaagcggc ggttccggcg
     5821 catcgaagac atcctgcgtg tgcccggtat cggccggaaa accttcgatc ggctgcgcca
     5881 atcgattcga gtccgttagc ggctaccgac cggcgacaga accgcctaga ttcgggcgca
     5941 tggcccagac gattgaggtg cgcgacgcgg atgcatccga ccgcatcgcg ctgatcgact
     6001 tccaccgcag cctctaccag gaacaccgcg acaaggtggt ggccgccgaa gatctccctc
     6061 tcatcgaata tcgagactac gatcaggtgc ttcacgatga tctggacgcc ttgcttcgag
     6121 acaggagcgg ctatgtgctg ctcgccgaat cggagggcgc cgcgctaggt tacatcacgg
     6181 gtcgcgtccg tacggagccg gatcgtgtct tgccacgccg cggagtcatc gaggattggt
     6241 tcgtcgtgcc cgaggcgcgc ggttcaggcg tcggcgcaaa gctgcttcat gcgctcgaaa
     6301 aacggtttgc cgccacgggt tgcgaggtga tcgaatccgc aacctgggcc ggaaacacgg
     6361 gcgcgaggca tgcacacgac gctctcgggt ttcgggagat cagggtaatg taccgcaagc
     6421 gggtttgacc cggacgctgg cgctgtggag atcggagggc ggctcgcgac gcacgtgctc
     6481 gcgattggga cccgctcggc cgtctctgta cgactccgta cgtctagcct cccgcccaat
     6541 cgctgtgccg cgcatcccga gccgccctcc tttgtacacg gcgaagcacg cccacggcac
     6601 taacgctgac actggcgctg acactggcgc tggcactggc gctggcgctg gcgctggcgc
     6661 tgggagtgta cggaggggcg gggcggaacg cgatgtggga ttccggcggc gtagcgagtg
     6721 acaatcggtc aaaaagcaac cacctccgat tggcgcgagc tccgaggcga ggcttgcgtg
     6781 gcccccggcc tcaagtccta ccggccacaa ttgcaagccg agccgtccga cggagtcccg
     6841 cgtcgtgttc cgccccgccc cgatcgccca atcgcgagca cgtgcgtcgc gagccgttct
     6901 ccggtgatac agtcgcgcat cacgtgctgt cagtggacgg cgggacggtg cgggagttcg
     6961 cttacgatct tggcctctgc gtcgaggagc tcgtcgaggc gcgcctggac tcgcggcgtt
     7021 ggaaagtact ccttggcgag acgaacgaac aggccgtggt gtctcgcttc cgatctcgtg
     7081 atctcgacgt agaaatcctt caaggctccc ggttcgaggg cgtctgctac gaggccgaag
     7141 cgctcgcaac ctcgggcttc gacgacaccg aggacgagga gccggtcgag aaaatactgc
     7201 tccgggccgc gtttgatcag ttgcatgagg gcgccaacgt acgggtcctt ctcgtccgcg
     7261 cgcgtctcga ggcctcgctc gaggatgatt ttcatgacct gggcgtagtg gtcgagctct
     7321 tcctgagcga aggggatcag ctcgcgtacc aagagggtgc ggtccgagta gtgtgagacg
     7381 agtttgagcg cggtcgccga ggcttttcgc tcacaggcgg cgtggtcgac gaggaacgag
     7441 tcgaagtcct ccaagacgac ttcgatccag gacgggctgg ttgcgagacg tagtcgcggc
     7501 atctgctctc acctaggcgc ggagcgcatc gaggattggc tggatggcgt cgcgccggag
     7561 cttcagggca gcgcggttcg cgattaccac cgagctgatc tcggcgattt tctcgagcgg
     7621 caccaggccg ttctggcgca gcgtctcgcc ggtttccacc aaatcgacga tcacatcggc
     7681 gagaccggtg agcggggcga gctcgacgga accctggcag aagatgagct cgacgggcgt
     7741 ccccttcctt tgaaagtact ggcctgcgat atgaggaaac ttcgttgcca cgcgcagcgg
     7801 acgttcacct tttccctcga tcggctcctt ggggcgcccg cagaccatca tctgacactt
     7861 gccaatatcg agatcgaccg gggcgaatac gtcgtagttg cgttccatca agacgtctcg
     7921 gccgacgatc cccagatctg cggccccgta ctctacgtag gtggggacgt cgtcgggctt
     7981 gagtagcaag taactgagcc cggcttcttt gtcctcccga acgagctttc ggctcttcgc
     8041 ggtgagggct tcggtggaaa tgccagcctt ctcgaaccgg gcactcagtt gttctagcac
     8101 tcggcctttg ggaaccgcga tgacgatgtt gtctcgattg cgtctaggca ttgccggcta
     8161 ctcgctcgat gttggcccca agtggggcca gctttttctc gatctgctca taaccccgat
     8221 cgaggtggta cacccgccga acgtaggtcg tgccctttgc gaccagtccc gcaacgacca
     8281 aggacgcgct ggcccggagg tcggtagcca tcacctcggc gccttcgagg cgatcaacgc
     8341 cctgcacggt cgcggtccgg ccattgacct gaatcctcgc gcccatgcga ttgagctcgg
     8401 agacgtgcat gaagcggttt tcgaataccg tctctgtaat gcgactcgtc ccttcggcga
     8461 ggcacatcag catcatgaac tgagcttgca tatcggtcgg aaacccggga tgcggttggg
     8521 tgatcacgtc cgtcggacga atcgggcgca caccgacgac gcgaatgccg tcgccctcgc
     8581 gctcgatcac ggttccggat tcgcgaagct tagcgatcac agcctcgagc ggctcgaagc
     8641 caattccctc gaggagcagg tctcccgatg tagctgcagc tgcaaccatg aaggtgccgg
     8701 cctcgatgcg gtccgggatg atcgcgtggt cgatcggatg aagagagtcc acaccttcga
     8761 tcgagatcac cgacgtgccc gctccttcga tgcgcgctcc catcttgttg aggacacggg
     8821 cgagctcttc gatctccggt tcgcgcgcgc agttcgtgag cgtggtgcga ccccgagcca
     8881 agaccgccgc ggacatgagg ttttcagttc cggtgtgtgt cgggaagtcg agggcgatat
     8941 cggatccccg gagtcttccg cctggaacgt tgacttcgac gtagccatgg tggagctcga
     9001 ctttcgcccc catcgccgaa agacccttga gatgctggtc gatgggtcga gccccgatcg
     9061 cgcagcctcc aggcagcgac accatggcct ttccgcaacg cgcgaccaga ggtccgagaa
     9121 ccaagaccga tgcgcgcatc tgcttcacca gatcgtaggg agccgtcggc tcgatcggtc
     9181 cggacaaact cacgtgcacc cggccatcga cgtactcggc cttggcgccc aatcgttcga
     9241 gaaggctcgt catcgtatgg acgtcacgaa gattgggaac gttacgaaac acgtgctcgc
     9301 catccgccag aagggcagcc gtcatgatcg gcaaggccgc gttcttcgcg ccgctgatcg
     9361 gcacggaacc cacgagcctg tttcctccga tgattcgaat gctgtccacg acgcccctgt
     9421 taccccgtgg ccccgcttcg ggcaacttca ggcgaagagc aatccgaggg caaatgaaac
     9481 gaacccgatc acggcgccgc aaagcggagc cctcgcaccg gcccagcgaa agcgcccgct
     9541 cgagtcgaaa ccgcgtcgca ctagaatcag caacgccaca atccagcccg tcattcccaa
     9601 taagagcagg ctgccccacc atggactagg agagactcgt tccgagagga ggcggcgatg
     9661 gtctgcagcg agttgctctt cgctcgaacc ggcatcgatg cccggggctc gatccttggc
     9721 catcagccgc gcgatctgtg cgtctgcttt ctctcgccga ggatcctccc cggagtagag
     9781 aaaacgcgct gcagccagac caccagagag cgatcgccaa gccaacaagg ccccttgcgg
     9841 atcgccc
//
LOCUS       DASRZF010000319         9211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4035317, whole genome shotgun sequence.
ACCESSION   DASRZF010000319 DASRZF010000000
VERSION     DASRZF010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 9211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9211
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4035317"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VFG22_14020"
     CDS             <1..286
                     /locus_tag="VFG22_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417409.1"
                     /translation="NAMSLLQRAGQETLVIYVIHLLLLYGSPISPGLNTLLDGALNLQ
                     GASIVAALVLLLSTAVALLWSYGRRRWPTRFRSFRYALAALVLFVALVKP"
     gene            353..1237
                     /locus_tag="VFG22_14025"
     CDS             353..1237
                     /locus_tag="VFG22_14025"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET6417410.1"
                     /translation="MIHFWLRLPGFHDVNSLENRNPRVIQRWAEIAQPILWRWFRPEV
                     RGLERIPEGSALYVCNHSGGFMTPDTWIFTITLLRERGVEAIPYALAHQAVIEAPGTN
                     QILTAWGTLAASPENAHRVFAAGFKVLVYPGGDLDVFRPSRDRNRIIFGPRRGYVRLA
                     LREAVPIIPVVSAGGHSGWWVLSDGRWLSRLLHTHRFLRTDVLPITLSLPWGLTVGAP
                     PYLPVPTRVLLEVLEPICFERSGPEAADDEEYVEACHDRVLETMQSALDRLTQERRTR
                     RRERLRGWIGETLYRGSE"
     gene            complement(1300..1920)
                     /locus_tag="VFG22_14030"
     CDS             complement(1300..1920)
                     /locus_tag="VFG22_14030"
                     /inference="COORDINATES: protein motif:HMM:NF039596.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6417411.1"
                     /translation="MGSKSRRLPASERRAQLIEVGRSVFAKHGYEGTSVEEIAKRAKV
                     SKPIVYEHFGGKEGLYAVIVDREMDYVERRISEAISSGSPRERVERASLAFLSYVRDH
                     PDGFAVLSQDSPLSSSRGTISSLLNDLAERVGDVFVSALKEAGYDTKAAPIYANALIG
                     MVTFVGTWWTEERKPPIEEVAKHISALAWMGLRHLPKRPTLQRSAD"
     gene            2084..3184
                     /locus_tag="VFG22_14035"
     CDS             2084..3184
                     /locus_tag="VFG22_14035"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HET6417412.1"
                     /translation="MVSMSKMGAVWRSAGSTVAERLFLDRQAEFWIGELDPVRSFGEL
                     RARVVEVIEETRDTKTFVLRPNAAWRGHRAGQYTSVEAEINGVRVRRCYSISSGPSEA
                     LVSITVKRVADGRVSTWLHENVRAGHILGLGAPAGDFVLPSPTPDKLLLLSGGSGITP
                     VMSMLRDLAVRDAIRDLVFVHHARSHADVIFGEELADLAARHSGLRLVLCLDDDASSP
                     RGFDEAYFAKLVPDFAERSTFLCGPKPLMDRAERLFESVGASDRLELERFTPALRLLP
                     TVTSVDGVQVRLGRSERNVVAKGQGSLLEQLERAGERPAHGCRIGICHTCKCRKQSGT
                     VENLVTGEVSSEPDQEIQLCISMPRSDLELDL"
     gene            3196..4338
                     /locus_tag="VFG22_14040"
     CDS             3196..4338
                     /locus_tag="VFG22_14040"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HET6417413.1"
                     /translation="MNTQSRNLTREEADAFGRELDALRQETVADLGERDVAHIRQVIR
                     TANRSEAAGRVLLHFGLGPVSFVLGTSALGLSKILENMEIGHNIMHGQYDWTRDPALD
                     SHRYEWDNVCTGDDWRHSHNYEHHTFTNILGKDRDVGYGFLRVTPEQEWEPIHLTQPI
                     VAAFLALFFQWGVGGHDLRIDELFSGKQTPAQLGKRARPFLTKAAWQLFKDYAFFPAL
                     ALGNAPRVFLGNLFANGTRNLWTFAVIFCGHFPEGVRVYEQTETENESRGQWYVRQLN
                     GSANIEGNRWLHVLTGHLSHQIEHHLYPDIPAARYPEMAPRVREICARYGQSYNTGSF
                     RRQLSSVIGRIVRLSLPGPKLAEKPMRPASGEVAAAHFASAGRAAA"
     gene            complement(4372..5448)
                     /locus_tag="VFG22_14045"
     CDS             complement(4372..5448)
                     /locus_tag="VFG22_14045"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6417414.1"
                     /translation="MGRRAGVETASVPVTAFVITGLAAQLANAIASIPFRGPWVGGSS
                     RLENMGIAVTREVVRSFIGYAVSLPTPEFRSLEIVINDLCRATLSPLLRPLDVALTDI
                     EIGGVPALSCRPRGGRPRGQILYLHGGGYIGTSPAMYLMFTGWLAHETQCEVLIPDYR
                     LAPEFPFPASLVDAAAVYEGLLAAGHSPDRLFVAGDSGGGGLVNALILDARAEHLPAP
                     AGLLLFSPEVSLTLDEPSITGNASMDILPWNIPVQPYLHGVASDDRRHSVNDANLRRF
                     PPTFVAYGEDEMFRDAIREFVDRLKQSKIETMVVEEPHMFHVFPILMPWAQASRRVYQ
                     AAQEFVTLQLSRATTAACEVQSVS"
     gene            5599..6225
                     /locus_tag="VFG22_14050"
     CDS             5599..6225
                     /locus_tag="VFG22_14050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417415.1"
                     /translation="MSNNEPPRGKSWHSARTLGLRIGAVLLALIGCDRQPAEEATPKS
                     TAPVREDLLIGYGLLADTLSDESHLGTLDLFKTLTLNAPNDAIAKMMQTLSEASTRRA
                     DELSKLRHLAPDVSDDPTQASPIGDAITAVAKEFGKSEMLSRNGGFDVRFVLLQAQAT
                     RMVAAMATAIARFEPNPQRKKWLKSLANEYEGYRGDLIKYLSVRADSP"
     gene            complement(6253..7101)
                     /locus_tag="VFG22_14055"
     CDS             complement(6253..7101)
                     /locus_tag="VFG22_14055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417416.1"
                     /translation="MRAVLRLLLWVALSMFLLLVLSGALLVWTSPGRGILAGWVEDLA
                     SSQIPGSMKIGKLESVGFIHPVATDVEFFTPGGERVIRVDTVEAQWNLKDLVKGKVGF
                     DAARADGGEVLIEIKEGGRTNMEAAFKSPEGEGEEKAQLQLDNMHFQGMTVLLRLSGE
                     TRFVVKDVQGFLSVWRRGTPGVRVSLARVTGTFEKPEITGDKIELIDMHGEVWAQETH
                     VVSMKLKTLIGGGGIDASFDYYKREENPAELILRPETGSGARLAAMVIHVRSLFSDKL
                     HVTIAN"
     gene            complement(7218..8261)
                     /locus_tag="VFG22_14060"
     CDS             complement(7218..8261)
                     /locus_tag="VFG22_14060"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET6417417.1"
                     /translation="MTGASQRLTVLASLSVVLVVLHLGRPIVIPFLLGLLIAIGVSGV
                     LRLSDRGIPRWLTVSLACVVAVGLCAGLGWLFAIAIDDLAADWPTYREAWNRLQIQIV
                     RWFWRHRLGASATVVRDLDPEETAMDLALPGIAVASRIGSSILLALMIAVFLLIEGAA
                     VSPKLKRMEKLGVLDAAVFREAASKVQRYLWIKTATSFATGVLAGALTAMVGLRHALL
                     FGLLAFVLNFIPNVGSLIAMIPAVVLGVLTLGWPTGLALIAGYALINLAIGVILETRW
                     MSEAAGLSPSIVILSMVFWGFVLGPAGALLSVPLMMIVRITAGQSPDWAWLEILLESP
                     RRVFKEEKKSQPP"
     gene            complement(8248..8376)
                     /locus_tag="VFG22_14065"
     CDS             complement(8248..8376)
                     /locus_tag="VFG22_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417418.1"
                     /translation="MSIGKPESVGCAHPLASKVELASKRCAWKSKIETEQLVHDRS"
     gene            complement(8820..>9211)
                     /locus_tag="VFG22_14070"
     CDS             complement(8820..>9211)
                     /locus_tag="VFG22_14070"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HET6417419.1"
                     /translation="MECFLVGAVIVAAATGRFQTLAIVWLGPALIAVAALALAFDFLP
                     HYPYESQERYLDTRVYPGRILNALLLGQNYHLVHHLWTTIPWYRYQRVFGEIQDELAE
                     RGARIEWRKEPAGQTMPSAGLSNAGSD"
BASE COUNT         1786 a         2918 c         2839 g         1668 t
ORIGIN      
        1 gaacgcgatg agtctccttc aacgggccgg ccaggaaacc ctcgtgatct acgtcattca
       61 cctgttgctg ttgtacggat cgcccatcag tcccggcctc aatacgcttc tagatggggc
      121 gttgaacctc caaggcgcat cgatcgtcgc cgccctcgtg cttctgctca gcacggccgt
      181 ggcgctgctg tggagctacg ggcgccgacg atggcctacc cggtttcgtt cgttccgcta
      241 cgccctcgcc gccctcgtgc tgttcgtcgc cttggtcaaa ccttgaggtg gcccctgtgc
      301 gactcgagaa ggccggcgtt gtggcgttgc aagtgacgcg gtactgtcaa tcgtgattca
      361 tttctggctt cgcttgcccg gttttcacga cgtcaactcc ttggaaaacc gcaatcctcg
      421 ggtgattcaa cgttgggccg agatcgctca gcccatcctg tggcgatggt ttcggccgga
      481 ggtgcgcggc ctcgaaagga ttcccgaagg cagcgcgctc tacgtgtgca accatagtgg
      541 tgggttcatg acgcccgaca cttggatctt caccatcacg ctgctccgtg agcgcggcgt
      601 ggaggccatc ccgtacgccc tcgcccacca ggccgtgatc gaggcaccgg gcacgaacca
      661 aatactcacg gcctggggca ccctggcggc gagccccgag aacgcacacc gggtattcgc
      721 cgcgggtttc aaggtgctgg tgtatccggg cggcgacctc gatgtctttc gtccgtctcg
      781 cgaccgaaac cgcatcatct tcggtccgcg ccggggttac gtgcgtctgg cgctccggga
      841 ggccgtgccg atcatccccg tggtctccgc gggtggtcac agcggctggt gggtattgag
      901 tgatggacga tggctctcgc gccttctgca cacgcaccga tttcttcgga ccgacgtcct
      961 tccgatcacc ttatcgctgc cctgggggct caccgtgggc gcgccgccct atctgcccgt
     1021 gccaacccgg gtcctcttgg aagtgctcga gccgatctgc ttcgagcggt ccgggccaga
     1081 ggcagcggac gacgaggagt acgtcgaagc ctgccatgac cgtgtactcg agacgatgca
     1141 gagtgcgctc gacaggctca cgcaggagcg acgcacgagg cgacgcgagc ggcttcgcgg
     1201 atggattggt gagaccctct accgcggcag cgaatagcgc ctgcatcgat cgcgtttccg
     1261 cgcgctcctg caggcccggc atcggaattc gatctcacgt cagtctgcgc tgcgttggag
     1321 tgtggggcgt ttggggaggt ggcgtagacc catccaggcc agggcactga tgtgtttggc
     1381 gacctcttcg atgggcggtt tgcgctcttc ggtccaccac gtgccgacga aggtgaccat
     1441 gccgatgagc gcgttggcgt aaatgggagc agctttggtg tcgtagccgg cttccttgag
     1501 cgccgagacg aacacgtcgc cgacgcgctc ggccaagtcg ttcagtaggc tcgatatggt
     1561 gccgcgcgat gagctcagcg gggagtcctg ggaaagcacc gcgaagccgt cgggatggtc
     1621 cctcacgtag ctgaggaatg cgagtgaggc gcgctctacg cgctcgcgtg gcgacccgga
     1681 cgagatggct tcgctaatgc ggcgctccac gtagtccatc tcgcgatcga cgatgaccgc
     1741 gtagagcccc tccttgcccc caaagtgctc gtagacgatg ggcttcgaga ctttcgcgcg
     1801 cttggcgatc tcctccaccg aggtgccctc gtagccgtgc ttggcgaaca ccgagcggcc
     1861 tacctcgatg agttgagcgc gacgctccga agctggtaga cggcggcttt tgcttcccat
     1921 tcgaccacag tactacgtat gcgtaagtta cgccatcggt aaataagcga gaaaacacag
     1981 atgcttagcc aatgtattcg gactcttaca aaacaaagac attgaccatt ccctacgaag
     2041 acgtaagtta cttcatcgta agttactgat acggaggtta gcgatggttt cgatgtcgaa
     2101 gatgggagcg gtctggagga gcgcagggag cactgtggcc gagcgcttgt tcctcgaccg
     2161 acaggcagag ttctggatcg gcgagctaga ccctgttcgg tccttcggcg agctccgcgc
     2221 ccgcgtggtc gaggtcatcg aggagacccg agataccaag accttcgtgc tccggccgaa
     2281 cgcagcctgg cgcggacatc gcgccgggca gtacacgtcg gtcgaggcgg agatcaacgg
     2341 cgtacgcgtg cgccgctgct actcgatctc gtcgggcccc agcgaggcgc tggtttcgat
     2401 cacggtcaag cgcgtggcgg acggccgcgt gtcgacgtgg ctgcacgaga acgtgcgggc
     2461 cggtcacatt ttgggcctcg gcgctcccgc cggtgacttc gtcctgccct ccccgacacc
     2521 cgacaagctg ctgctcctga gcggcggcag cggcatcacg cctgtcatgt cgatgctgcg
     2581 cgatttagcg gtgcgggacg cgattcgcga cctcgtgttc gttcaccacg cgcggagcca
     2641 cgccgacgtc atcttcggcg aggagctcgc ggaccttgcc gcacgccaca gcggcctgcg
     2701 cctcgtcttg tgcctcgacg acgacgcctc gtccccccgc ggattcgacg aggcgtactt
     2761 cgcgaagctg gtccccgatt tcgcggagcg ctcgacattt ctctgcggcc cgaagcccct
     2821 catggaccgc gctgaacgac tcttcgagag cgtcggcgca tccgatcggt tggagctcga
     2881 gcgattcacc ccggcgctgc ggctgttgcc gacggtaacc tctgtagacg gtgtgcaggt
     2941 gcggctcggg cgctctgagc gaaacgtcgt ggcgaagggc caaggcagcc tgctggagca
     3001 actcgagcgt gccggcgagc gcccggctca cggttgccgc atcggcattt gccacacctg
     3061 caagtgccgc aagcagagcg ggaccgtgga gaacctggtc accggggagg tgtcgagcga
     3121 gcccgatcaa gagattcaac tgtgtatttc aatgccccgc tccgacctgg agctcgacct
     3181 ctgaaaggaa accccatgaa cacccaaagc agaaatctga cgcgcgagga ggccgatgcg
     3241 ttcggccgcg agctcgatgc gctccgacag gaaactgtcg cagacctcgg cgagcgcgac
     3301 gtggcgcaca tccgccaagt catccgtacg gccaaccgaa gtgaggcggc cggccgggtg
     3361 ctcctgcact tcggtctcgg acccgtgagc ttcgtgctcg gcacgagcgc gctcggtctc
     3421 tcgaagatcc tcgagaacat ggagatcggg cacaacatca tgcacggcca gtacgactgg
     3481 acgcgtgatc cggcgctcga ttcgcaccgc tacgagtggg acaacgtgtg cacgggcgac
     3541 gactggcgtc actcgcacaa ctatgagcac cacaccttca caaacattct cggcaaggac
     3601 cgcgacgtgg ggtacggctt tttgcgagtg actcccgaac aggagtggga accgatccac
     3661 ctcacgcagc cgatcgtcgc tgcattcctc gcgctgtttt tccaatgggg cgtgggaggt
     3721 cacgaccttc gtatcgacga gctgttttcg ggcaagcaga cgcctgcgca gctcggcaag
     3781 cgcgcgcggc cgttcctcac gaaggcggcc tggcagctct tcaaggacta cgcgttcttc
     3841 ccggcgctgg cgctcggcaa cgcgccgcga gtgttcctcg gcaacctctt cgccaatggg
     3901 acgcgcaacc tctggacctt cgcggtcatc ttctgtggtc atttcccgga gggcgtgcgc
     3961 gtctacgagc aaactgaaac cgagaacgag agccgcggcc aatggtacgt gcgccagctc
     4021 aatggctcgg cgaatatcga ggggaaccgc tggctgcacg tgctcaccgg ccacttgagc
     4081 caccagatcg agcaccacct ctacccggac atcccggcgg cacgctaccc cgagatggct
     4141 ccacgcgtcc gcgagatctg cgcgcgctat ggacagagct acaacacggg aagctttcgg
     4201 cgccagctga gcagcgtcat cggacgcatc gtgcgcctgt cgctacccgg gccgaagctt
     4261 gcggagaagc cgatgcgccc ggcgtcggga gaagtcgcgg ccgcccactt cgcgtctgcc
     4321 ggccgcgcgg cggcgtgatg caacacccaa gcggtgtgtc gtgtaggcgc ttcacgaaac
     4381 tgactgaacc tcgcaagcgg cggtggtagc cctcgacaac tgcagcgtca cgaactcctg
     4441 tgccgcttgg tacaccctgc ggctcgcctg tgcccagggc atcaggatcg gaaacacgtg
     4501 gaacatgtgt ggctcctcga cgaccatggt ctcgatcttg ctctgcttca gtcgatcgac
     4561 aaattcgcgt atcgcatcac gaaacatctc gtcctcacca taggccacga acgtaggtgg
     4621 gaagcggcgc aggttggcgt cgttgactga gtgccgccgg tcgtcggaag cgacgccgtg
     4681 caggtacggc tgaacgggaa tgttccacgg aagaatgtcc atggaggcgt tcccggtgat
     4741 cgagggctcg tcgagagtga ggctcacctc gggggagaag agcaggagcc ccgctggcgc
     4801 gggcaggtgc tccgcgcgag cgtcgagtat cagcgcattg acgagcccgc cgcctcccga
     4861 atctccggcg acaaatagtc gatcggggga gtgtcccgct gcgaggaggc cctcgtacac
     4921 ggcggcggcg tcgacgaggc tggcaggaaa cggaaactcc ggtgcaaggc ggtagtccgg
     4981 aatcagcact tcgcattgag tctcgtgcgc gagccaaccc gtgaacatga gatacatggc
     5041 gggggaggtg ccgatgtaac caccgccgtg gaggtagagg atctggcctc gcggtcttcc
     5101 accacggggt cgacaggaaa gcgccgggac gcctccgatc tctatgtccg tcagcgcgac
     5161 gtcaagtggc ctgagcaagg gggacagagt tgcgcggcac aaatcattga tgacgatctc
     5221 gagcgatcga aactctggcg ttgggaggga caccgcgtat ccgatgaagg atcggacgac
     5281 ttcgcgtgtc accgcgattc ccatgttctc aagccgactc gacccaccga cccatggccc
     5341 gcggaatgga atcgacgcaa tggcgttggc aagctgcgct gccagcccgg tgatcacaaa
     5401 ggcggtgact ggcaccgatg cagtctccac gcctgctcgt ctacccatta ccatgatgca
     5461 ccgccttcgc tgctccttat cgtaccgaac ctcgagccat gcaactggcc cgcgcagcat
     5521 cgcaggcgtt ttgcctcggg tttcgtggac tacacggttc agagtgccta tctccagaac
     5581 atcgagtagg aggaacgaat gtcgaacaat gaaccgccta ggggcaaatc ctggcattcc
     5641 gcgaggacgc tcggcctgcg catcggtgcc gtgctgctgg cgctgattgg ttgcgaccgg
     5701 cagcctgctg aagaagccac acccaagtca accgcacccg tacgcgagga cctgctcatc
     5761 ggctatggcc tgctggctga caccctgtca gatgagtcgc acttggggac tctggatctc
     5821 ttcaagacgc tgacgctcaa tgctccgaac gacgccattg ccaagatgat gcagaccctg
     5881 agcgaggcgt cgacacggcg cgccgacgag ctttcgaagc tgcgtcacct cgcgccggac
     5941 gtgtcggacg atccgacgca ggcaagccca atcggtgacg cgatcactgc ggttgccaag
     6001 gaattcggca agtcggagat gctcagccgc aacggcggat tcgacgtccg ttttgtgctc
     6061 cttcaggcgc aggcaacccg aatggtggct gccatggcga cggctatcgc gcgcttcgaa
     6121 cccaaccctc agcgcaagaa gtggttgaag agcctcgcaa acgagtacga aggctaccgc
     6181 ggcgacctga tcaaatacct cagcgtacga gcggactcgc cgtagcccga accttgggtc
     6241 agggtcgtct cttcaattcg cgatcgtgac gtgaagcttg tccgagaaca aggaccggac
     6301 gtgaatgacc atcgcggcaa gccttgctcc cgaaccggtc tccgggcgga gtatgagctc
     6361 ggcggggttc tcttcacgct tgtagtagtc gaaggaagcg tcgatccctc ccccgccgat
     6421 gagggttttg agcttcatcg agaccacatg agtttcttgc gcccacacct ctccgtgcat
     6481 gtcgatcagc tcgatcttgt cgccggtgat ctcgggcttc tcgaaagtac cggtcacccg
     6541 cgcgaggctg actcgcacgc ccggtgtccc tcgtcgccac acgctgagga agccctgaac
     6601 gtctttgacg acgaatcggg tctcgcccga caagcgcagc aagaccgtca tgccctggaa
     6661 gtgcatattg tcgagctgga gttgagcttt ttcctcgccc tcgccctcgg gagacttgaa
     6721 cgcagcctcc atgttcgtgc ggccaccctc tttgatttcg atgaggacct caccaccgtc
     6781 ggcgcgcgcc gcgtcaaaac cgacctttcc tttgacgagg tctttgaggt tccactgcgc
     6841 ctctactgtg tcgactcgaa tcacgcgctc gccgcccggc gtaaaaaact ccacgtcggt
     6901 cgctacaggg tggatgaaac cgacggactc gagcttgccg atcttcatcg atccaggaat
     6961 ctgcgagctc gccaagtctt cgacccaccc agccaagatc ccgcgacccg gcgaagtcca
     7021 tacgagcagt gccccgctaa gcacgagtag caggaacatc gacaacgcga cccagagcag
     7081 caagcgcaag acagcgcgca ccgtgcgatc atgcccgaca accacgaatc agcgccactc
     7141 aaaaatcgat cgaccgggga cttcgacaag tgaggctctt tggcccggcg cctccgccgc
     7201 aacggcgtgg cgttgcgtta cggcggctgt gactttttct cctccttgaa gacgcgcctc
     7261 ggcgactcga gtaggatttc gagccaagcc caatcgggcg attgccccgc cgtgatgcgc
     7321 acgatcatca tcagtggcac ggagaggagc gcgcccgcag gcccaagtac gaagccccaa
     7381 aaaaccatcg acaggatgac gatactgggc gagagccctg ccgcctcgct catccaccgg
     7441 gtttcgagga tcaccccgat cgccaggttg atgagagcgt acccagcgat gagtgccaga
     7501 ccggtcggcc aacccagcgt cagcacgccc aaaacgaccg cagggatcat ggcgattaga
     7561 ctgcccacgt tggggatgaa gttgagaacg aacgcgagaa gcccgaacag caacgcgtgt
     7621 cgcaagccga ccatcgcggt cagcgccccc gcaagtacac cggttgcgaa gctcgtcgcg
     7681 gtcttgatcc acagatagcg ctgcaccttg gacgctgcct cacgaaagac agcggcgtcg
     7741 aggacgccca gcttctccat ccgtttgagc tttggtgaca ccgcggcccc ttcgatcaac
     7801 aaaaagacgg cgatcatcag cgcgagcaat atcgaggaac cgattcggct tgccacggcg
     7861 attcccggta gggccagatc catggcggtc tcctccggat cgagatcgcg cacgacggta
     7921 gcggacgcac cgagtcgatg gcgccagaac caacgaacga tctgaatttg aaggcgattc
     7981 catgcctcac gataagtcgg ccaatcggcg gcaagatcat cgatggcgat ggcgaacaac
     8041 cagccgagtc cagcacagag tcctacggcg accacacagg ccaaggaaac ggtcaaccaa
     8101 cgcggtatgc cccggtcact gagccgaagg acgccagaga ccccgatcgc gatcaagagc
     8161 cctaggagaa acggaatgac gatgggtcgc cctaggtgaa gaacgacgag cacgactgac
     8221 agagacgcga gcaccgtcag gcgttgacta gctccggtca tggacgagtt gctcggtctc
     8281 gattttcgac ttccatgcgc atctcttcga agcgagctcg actttgctgg ccaagggatg
     8341 ggcgcaaccg accgattcgg gctttccgat gctcatcgac ctcgcgagaa ccccctgcac
     8401 tgactccacc gagcgcgagg atatcgaaga tgcaccgtac cagccagcac aaggacattg
     8461 cgacgagagc ttagccggaa cagaggccct ctcggcgcgg cccccccacg cgacatatca
     8521 actagttctg cgcaattggg ctctgcacat gggtcttgct ggctaataga gttccggctt
     8581 cgggcgcaga gtgctggcgc gtgggtgcac ctctgaccgc ttccactcaa cccgagcgcg
     8641 ccacggctcg cgacggtcaa tgcgactcct gtctctcagg aactggaggg cacgtaagta
     8701 agaggccggc ttggatgccg ttgacagcga tacgcgagaa acccgagtgt ccgcgtaggg
     8761 ctagcgagag ctagggggtc gtcgctttcg aatgatcgtt tcaccctagt gacaccgcat
     8821 caatcgctac ccgcgtttga caggcctgcg ctcggcatgg tttgccccgc cggctccttg
     8881 cgccactcga tgcgggcccc acgttccgcc aactcatctt ggatctcacc gaatacgcgc
     8941 tggtagcggt accaagggat ggtcgtccag agatgatgga cgagatggta gttctggccg
     9001 agcagcaacg cattgagaat ccgccccgga tagacccgtg tgtcgagata acgctcctgc
     9061 gattcgtagg gatagtgcgg caggaaatcg aaagccaacg ccaaggccgc gaccgcgatg
     9121 agagcggggc ccagccagac gatggcaagt gtctggaatc ttcctgttgc cgcagccaca
     9181 atcaccgcac cgaccaagaa gcattccatc g
//
LOCUS       DASRZF010000320         2993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3000074, whole genome shotgun sequence.
ACCESSION   DASRZF010000320 DASRZF010000000
VERSION     DASRZF010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3000074"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VFG22_14075"
     CDS             complement(<1..339)
                     /locus_tag="VFG22_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417420.1"
                     /translation="MADVRFPSSLDELTPEVVTALLGEQRPGARVTNLRVLRSANRGD
                     GVASTADRVALELAYESDVGLPSRMLLKTVLLHRSLRFGASSIQWTGRLFGLLGALPL
                     GSRVRPAVFSL"
     gene            complement(350..775)
                     /locus_tag="VFG22_14080"
     CDS             complement(350..775)
                     /locus_tag="VFG22_14080"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET6417421.1"
                     /translation="MITYETETTIDASPSEVWKHLADFARHDEWSRHFKLRGRPVAGE
                     TGRVEFTLFGKTSQVDVRIEKVEELRELRWRGGPKGLVTGSHYFILEGLDGGNRTRFR
                     HGETFGGLLAPIVWAFLKAQLGPSYAGFNEDLKRRVEGA"
     gene            complement(826..2331)
                     /locus_tag="VFG22_14085"
     CDS             complement(826..2331)
                     /locus_tag="VFG22_14085"
                     /inference="COORDINATES: protein motif:HMM:NF017809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HET6417422.1"
                     /translation="MPVFTPDHIQAFLRQDARLDGHFVAGVTSTRVYCKPSCSAKKPV
                     PEDVELFDTPAEAEAAGFRACRVCQPRTSPDAIASDGTSTTVSRALRLIEDGVLDHAS
                     IDALCERLDIGARQLRRLFHKHLGTSPVQVARIRRAHFARRLIETTSLSMANIARAAG
                     FGSVRRFNAVINEVYGCPPTALRKAPTTRPARLELEIPVKGPFPWARMLEFLEPWTAS
                     GVEQVVGDRYYRTASFGKVAGEICVTYAPNVGALHVRVSRSLGENLLDVISSVRRLFD
                     VDAPTDEIAAHLGGDLALRARIESTPGLRVPGAFDHFETAVVMLMNQHMRPEEASDLM
                     DRVVEKYGTRIETSQPSLTHVFPSPYVLSTASLEAVGVPKRRARSIQALARAVHEGAL
                     RLDGSPSLEAAIESLRSISDVSATSAEYIAMRVYRESDAFPSNNHWLRSAVAEDGPSV
                     SIQELESRAETWRPWRAYAAMYLWDSYLADERDARRLWDRDSAPPEATQVA"
     gene            complement(2471..>2993)
                     /locus_tag="VFG22_14090"
     CDS             complement(2471..>2993)
                     /locus_tag="VFG22_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417423.1"
                     /translation="CGTGESCDPTSAGDICRPTGTGTEGSVCGSVNACEAGLGCVAVP
                     TDAATTYCRPLCDAGSGVSCVDGNTCIGLTAPPNLGGCLLDSCDLYASACPSGSGCYP
                     TTAYGDVCWLAGAGAAGASCSNYTDCVAGTTCVEDSPGGPAFCREICDLGNPVCSGVE
                     VCTATTGSYGACQ"
BASE COUNT          599 a          969 c          922 g          503 t
ORIGIN      
        1 gagcgaaaac accgccggac gaacacgcga cccgagcgga agcgcgccga gtagtccgaa
       61 gagcctgccg gtccactgaa tcgaagatgc gccaaatcgc aagctcctgt gaagcagcac
      121 cgtcttgagc aacatccgcg acggcagccc gacgtcggac tcataggcga gctcgagcgc
      181 aacacggtcc gctgtcgacg ctaccccatc gccgcgattc gcgctccgca agacacgcag
      241 attcgtcacg cgtgcccccg gtcgctgctc gccgagcaga gccgtcacca cctcgggagt
      301 cagctcgtcg aggctcgatg gaaaccggac gtccgccatc atacgctcat caggcgcctt
      361 cgacgcgccg cttcaggtcc tcgttgaacc ctgcgtaaga aggcccgagc tgcgccttca
      421 agaacgccca gacgatggga gcgagaagcc caccgaaggt ctccccatgc cggaagcgcg
      481 ttcgattccc gccgtcgagc ccctccaaaa tgaagtagtg actgcccgtc acgagcccct
      541 tcggcccacc acgccagcga agctcccgta attcctcaac tttttcgatc cgcacatcaa
      601 cctgggaagt ttttccgaag agggtgaact cgacgcgccc ggtttcgccg gcgaccggcc
      661 ggccgcgcaa cttgaagtgc cggctccatt catcgtgccg cgcgaagtcg gcaagatgct
      721 tccacacctc gctcggcgac gcatcgatcg tcgtttcggt ttcgtacgtg atcattgcgc
      781 cgagcgtgtc acgactcggg cgctcggacc agtgccgacg acgcgctacg cgacctgagt
      841 tgcctcgggc ggagccgaat cccgatccca aagtcgccga gcatcgcgct cgtcggcaag
      901 gtaggaatcc caaagataca tggcggcgta ggctcgccaa ggtcgccatg tctccgcacg
      961 gctctcgagc tcctgaatcg agactgacgg cccgtcttcg gcgaccgcgc tccgtagcca
     1021 gtgattgttc gaagggaagg cgtcggactc gcgatagacg cgcattgcga tgtactcagc
     1081 gctggtggcg cttacgtcgc tgatggaccg caagctctcg atggcggcct cgagcgaagg
     1141 cgagccgtcc aacctgaggg cgccttcgtg aaccgcgcgc gcgagcgctt gaatcgaacg
     1201 agcccggcgc ttgggcacac cgacggcttc caggcttgcc gtgctcaaga cgtaaggcga
     1261 agggaaaacg tgagtgaggg acggttgcga ggtttcgatt cgcgtgccgt acttttccac
     1321 gactcggtcc atgaggtcgg aggcctcttc cggacgcatg tgctgattca tgagcatgac
     1381 cacggcggtc tcgaagtgat caaaggcgcc gggcacacgt agccccgggg tcgattcgat
     1441 gcgcgccctg agggccaaat cgccgccgag gtgtgcggcg atttcatctg tcggagcatc
     1501 gacatcgaac aagcggcgca cactggaaat cacgtcgagc aaattttcgc caagagacct
     1561 gctcacccga acgtgcaggg caccgacgtt cggtgcgtac gtcacgcaga tctcccctgc
     1621 cacctttccg aatgacgcag tcctgtagta tcggtcgcca accacctgtt cgacacccga
     1681 cgcagtccaa ggctccaaga actcgagcat ccgagcccaa ggaaacggcc ccttgacggg
     1741 aatctcgagc tcgaggcgtg ctgggcgcgt ggtgggggcc tttcggagag ccgtaggagg
     1801 gcaaccgtac acttcgttga tcacggcatt gaaccgacga acgcttccaa agcccgccgc
     1861 ccttgcgatg ttggccatcg acagactcgt cgtctcgatc agacggcgcg cgaagtgcgc
     1921 gcgtcgaatg cgcgccactt ggacgggaga cgttcccaag tgcttgtgga agagccgccg
     1981 gagctggcgc gcgccaatat caagacgctc gcagagcgca tcgatgctcg cgtgatcgag
     2041 cacgccgtct tcgatcaggc gaagcgctcg ggatacggta gtcgaggttc cgtctgacgc
     2101 gatcgcatcg ggcgaagttc gaggctggca tacgcgacac gcacgaaagc cggccgcttc
     2161 cgcttcggcg ggcgtgtcga acaattcgac gtcttcggga acaggcttct tcgcggagca
     2221 gcttggctta cagtacacgc gcgtggacgt cactccagct acgaagtgac catccaaacg
     2281 ggcatcctgg cgaagaaacg cctggatatg atccggcgtg aatacgggca tggggggaac
     2341 aaaaagaata aaccgttttc gacaatagcg ttagcacttt tcggacactt ctcggttgat
     2401 acagcgacct acatctgccg ggggagtcgg agtctgtgag atggctcaca catcgtgctg
     2461 cgctgcactt ctattggcag gcgccgtacg aacccgtcgt cgccgtgcag acttctactc
     2521 cgctacacac cgggttccca aggtcacaaa tctcgcggca gaaggccggg ccgccagggc
     2581 tgtcctccac gcatgtggtg ccggctacac agtcggtgta gttcgaacag gaggctcccg
     2641 ccgcaccggc gcccgcgagc cagcagacgt cgccgtaggc tgtcgtcggg tagcaaccgg
     2701 aaccgctcgg gcaggccgag gcatagaggt cgcagctatc gagcaggcag cctcccagat
     2761 tgggcggagc cgtcaggccg atgcaggtgt tgccgtcgac gcaagagacg ccgctgcccg
     2821 cgtcgcagag cggcctgcaa tacgtagtgg cggcatcggt gggaaccgcg acgcagccaa
     2881 gccctgcctc acaagcattc acagaaccac aaacagagcc ctcggtgccg gtcccggtcg
     2941 gacgacagat atcgccggcc gaggtcgggt cgcagctctc ccccgtcccg cag
//
LOCUS       DASRZF010000321         8117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4378913, whole genome shotgun sequence.
ACCESSION   DASRZF010000321 DASRZF010000000
VERSION     DASRZF010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 8117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8117
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4378913"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VFG22_14095"
     CDS             complement(<1..297)
                     /locus_tag="VFG22_14095"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HET6417424.1"
                     /translation="MDLLDRRFLFVVGKGGVGKTTVATGLALAASRRGKKVLLALVNC
                     KERVSQLLDTDPIGPDIVTVRENLDVVNMTPDAALEEYGLMILKVRAVFNAVFKN"
     gene            421..1119
                     /gene="ccsA"
                     /locus_tag="VFG22_14100"
     CDS             421..1119
                     /gene="ccsA"
                     /locus_tag="VFG22_14100"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HET6417425.1"
                     /translation="MSSTRQSGSSTVWSLVLFATLALFAATLYAIFIYAPVEQQMGIV
                     QKIFYFHVPSAYAMYIGFGVSAVGSAGYLLKRDARWDAVGVAGAEVGTLFCLIVLLTG
                     PLWARKAWGVWWTWDPRLTTTLLAGMIFAAYLALRSMGNAGEVEKRFAAGLALFGLVD
                     LPLIHYSVQRWRGVHPTVITGKGGGLESEMYWALGLSFIAFTGLAILLIWARASIERQ
                     REETAQLRFELMHR"
     gene            1130..1468
                     /locus_tag="VFG22_14105"
     CDS             1130..1468
                     /locus_tag="VFG22_14105"
                     /inference="COORDINATES: protein motif:HMM:TIGR04391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HET6417426.1"
                     /translation="MRALIAAIVLGCTILFTPVAIAQTQPDEPDAAAEAPEEAAATED
                     AAEERAAQFVGVRGPEAESVPGGALLLGAYAIVWLLLFLFLMRMRGLQRQTAEELERL
                     SLEIRASGDR"
     gene            1468..1596
                     /locus_tag="VFG22_14110"
     CDS             1468..1596
                     /locus_tag="VFG22_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417427.1"
                     /translation="MPSIAHIIYIPGILLIGFALGFRMGARAVRAELERREQERRR"
     gene            1612..2760
                     /locus_tag="VFG22_14115"
     CDS             1612..2760
                     /locus_tag="VFG22_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6417428.1"
                     /translation="MLDFELTDEQKALVEASRRFTREKIIPVAAQADKTHEFPMDVFN
                     EAWELGFVGPNIPEAYGGAGMGEVDHVLLTEELAFGCTGIQTSMTANVLAATPILIAG
                     SEAQKKKYLGMLTSEPVFASYAITESVAGSDSAGIQTRCRKDGNDWIINGEKIYITNG
                     AWARWYIVFATVDPSLRHKGIMAFVIDRDAKGVSVGKTEDKLGQRASNTAVINFDDVR
                     VSSDNLLAEQGHGFKLAMQTFDRTRPDIGAGACGLMRRALEESVAYALERKTFGVPIA
                     EHQMVQAMIAEMGIKYEATRLLVWKAAWQIDQGGRNSLVASYSKAFGADSAMQVATDA
                     VQVFGGNGYITEYPVEKLMRDAKILQIYEGTSQIQRMVIAKNLFAGVR"
     gene            2837..3688
                     /locus_tag="VFG22_14120"
     CDS             2837..3688
                     /locus_tag="VFG22_14120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417429.1"
                     /translation="MGSNVDQDELLEVISTAKSRSFKPVGHSSVVLRMRTVAHVTAGL
                     KERSKAIEDGYQYEIAAYRISRLLGLDNVPPAVYRRVAWREVQNRFHEDKLDQRASIR
                     RKVLWDDDGSAPAAAVYWVKGMKSIGLEKRSTWESWLRDGEIPESRAALARDLSTMVV
                     FDFLIGNWDRFSGGNLPIDSSRERAILRDNDRSFSNPLHERRYEMLLDGLTRTSRFSK
                     NLVRRLSELDEASLRAELAEDPSQATQPLLNDAQIADFLERRATVLSYIAAMIEDRGE
                     PQVLFFP"
     gene            3714..4421
                     /locus_tag="VFG22_14125"
     CDS             3714..4421
                     /locus_tag="VFG22_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417430.1"
                     /translation="MAKPKVIDPDEWGHVLGLLVHDLRNPAATITANVDFLQEVGLVD
                     TDSVEALQDVKLAVGELRRGLDMLAWISLWLSGQVPLEAANGDVGMFLERLRRVDTPV
                     PVSVEIAEGQQLYAQGAQAAVEVVELLLHNTRANVADPRATVRVFQQGGHVVIEVEDA
                     GEAIAPELRDGAFSLDGQRALKGRLDGRYSRFVGLFAAAVVLDGVGGTIEADGEKGAA
                     IFRIFLRGARPTRPPPR"
     gene            complement(4372..5721)
                     /locus_tag="VFG22_14130"
     CDS             complement(4372..5721)
                     /locus_tag="VFG22_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HET6417431.1"
                     /translation="MDLFERQAESSSPLADRMRPKRLEDVVGQAHLLGDGHILARAIR
                     ADRIPSMILWGPPGTGKTTLAHVIAYETHSIFVPFSAVLGGVPELRKLIAAAKEQKSY
                     YGKKSILFIDEIHRFNKAQQDALLPHVEDGTVTLIGATTENPSFAVNSALLSRARVFD
                     LKPLEASDLHRLLERALGDEEHGVGHVPHRFEADALAAISQNAGGDARRALSALELAS
                     DFAQSAGVETVTVGIVQEALATQTLLYDKSGEEHYNVISAFIKSMRGSDPNASVYWLM
                     RMLEAGEDPLFVLRRMLIFASEDIGNADPRALDLAVSSDAAFRRLGMPEGIFPIAQCC
                     IYLASAPKSNASYKAWTAAQTDVRELGALPVPLKLRNASTGAMKSWGYGDGYRYPHDE
                     SGFSTGETYLPDALVGRTYYRPTTRGFEARIRERLRELGERSSEEADESGEPPEGKS"
     gene            complement(5737..6843)
                     /locus_tag="VFG22_14135"
     CDS             complement(5737..6843)
                     /locus_tag="VFG22_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HET6417432.1"
                     /translation="MGSPTLDTVTDALRKVEDPIFQKPLGALGTLIDARLEDGRVWAK
                     VRVSSPSEEVRRTLHARVKAALEPLGIEFTELELEVNVPTREATSTDPIPDVRNVILV
                     MSGKGGVGKSTVATNLALALRRLGTRVGLLDADIYGPSLPTMLGIEGQPTSKDGKRIQ
                     PLQRFGIKLMSIGFLLEDPKQAVVWRGPMLHGALQQFVSDVDWGGLDYLIIDSPPGTG
                     DVALTLAQKLRITGAIIVTTPQEVALQDVYKSVSMCKKLNVPILGVVENMSHFIDSAG
                     VKHEIFGSGGGAKIAEFAEAPLFGQVPIDTLVREWGDQGTPIVQAAPASAAAQAFAGV
                     ADALAERIAKEHFDRGGGESAPEGEGPKRLRILR"
     gene            6948..>8117
                     /locus_tag="VFG22_14140"
     CDS             6948..>8117
                     /locus_tag="VFG22_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417433.1"
                     /translation="MSIWERSWAVPLAIFLLCSGVYCATAGGRLFRPSPNNHYVHLAD
                     AWLHGRLDLGAHAPGTNDWACFDTQDRGPCPSGRYSFSPPESTRYRWYVSFPPLPAMI
                     LLPLVAVFGLGTLDALFWAVFAGLAPMFLFTVLRYLRESGRSHRSLRDDLIITALFAV
                     GSVYYFVAVQGTVWFAAQVVASAFICLYLLASFGARRPALAGLALGLAFLSRPSTLLL
                     GAFFVLQAISAANERGQPSRRARAQLIGKLIKFALPLTFLLGAAMWHNAARFDDPFEF
                     GHRFLQVRWRSRIETWGLFGTHYLPRNLKVFFLSLPWILESNPFVRITRHGLAFWFTT
                     PNLVWSLFPKKLDSTIVALWAAVLPTAICTLLYQNTGWVQFGYRFALDYLPFLFVL"
BASE COUNT         1481 a         2467 c         2511 g         1658 t
ORIGIN      
        1 gttcttgaaa acagcattga acacggcgcg caccttcaag atcatcaagc catactcttc
       61 gagcgccgcg tccggggtca tattcacgac gtcgagattt tctcgaaccg tcacgatgtc
      121 cggaccgatc gggtcggtgt cgaggagctg gctcacgcgc tccttgcaat tgaccaaggc
      181 gaggaggacc ttcttgccgc ggcgacttgc cgcgagggcg agcccggtcg ccacggtggt
      241 cttcccgacg ccacccttgc caacaacgaa aaggaaccgc cggtcgagca aatccatctg
      301 cgccgtcttt agcggtgagc tggaagggcg gcaaaccgac cggatttaat cgggcaattc
      361 tgggatggga gaggctgacg cccaagcagc cgcatgctag cgtccggacg agcgcaatac
      421 atgagcagta cacgtcagtc cggtagctcg accgtgtggt cactagtgtt gttcgcaacg
      481 ctagcgctct tcgcggcgac tctctacgcg atcttcatct atgcgcctgt cgagcagcaa
      541 atgggcatcg tgcagaagat tttctacttc cacgtcccaa gcgcctacgc gatgtacatc
      601 ggttttggcg tttcggcggt gggcagtgcg ggatacctgc tcaagcgcga tgcgcgctgg
      661 gatgcagttg gggtggccgg cgcggaggtt ggcacactgt tctgtctcat cgtcttgctc
      721 accggcccac tctgggcgcg caaggcctgg ggtgtttggt ggacctggga tccacggctc
      781 acgactacgc tgctcgccgg catgatcttc gccgcatatc tcgcgctccg atccatgggc
      841 aatgctggcg aggtcgagaa gcgattcgcc gcagggctag cactcttcgg tctggtagac
      901 ctgccattga ttcattacag cgtgcagcgc tggcgtggcg tccaccccac ggtcatcacg
      961 ggcaaagggg gagggctcga gtccgagatg tattgggcgc tcgggctcag cttcatcgcg
     1021 tttactgggc tcgcgattct cttgatttgg gcccgcgcgt cgatcgagcg ccagcgagaa
     1081 gaaaccgccc agctacgttt cgagttgatg caccgatgaa gggaacgaaa tgcgagcttt
     1141 gatcgccgcg atagtgctgg gatgcaccat acttttcacg ccagtggcca tcgcccagac
     1201 gcagcccgac gagcccgatg cggcagctga ggctccggag gaagcggctg cgacagagga
     1261 cgctgcagag gagcgagccg cacagttcgt cggcgtacgc gggccggagg ccgagagcgt
     1321 tcctggcggc gctctgttgc tcggagccta cgcgatcgta tggctgctct tgttcctgtt
     1381 cttgatgcga atgcgcggcc tacagcgtca aaccgccgag gagctggaac gacttagtct
     1441 ggagattcgg gcctccgggg accggtaatg ccatccattg cacacatcat ttacataccg
     1501 ggcatattgc tgatcggctt tgcgctcgga tttcgaatgg gagctcgcgc cgtgagggcc
     1561 gagctggagc gccgcgaaca agaacggcgc cgttgatcga gaggtagcag catgttggat
     1621 ttcgagttga ccgacgaaca gaaggcgctg gtagaggcga gccgtcgctt cacgcgagag
     1681 aagatcatcc ctgtcgccgc ccaggcggac aagactcacg aattcccgat ggacgtcttc
     1741 aacgaggctt gggagctggg cttcgtgggg ccgaacatcc ccgaggccta cggtggcgcc
     1801 ggcatgggcg aagtcgacca tgttctgctg accgaagagc tcgcgttcgg ctgcacgggg
     1861 attcagacca gtatgaccgc caacgtgctc gccgcgacgc caatcctcat tgccggcagc
     1921 gaagcgcaaa agaagaagta cctcgggatg ctgacctcag agcctgtgtt cgcatcctat
     1981 gcgattaccg aatccgtcgc cggaagcgat tcggcaggca ttcagacacg ctgccgcaaa
     2041 gacggaaacg actggatcat caatggggag aagatctaca tcaccaacgg agcgtgggcg
     2101 cggtggtaca tcgtgttcgc cacggtcgac ccttcgcttc gccacaaagg gatcatggct
     2161 ttcgtcatcg atcgcgacgc aaagggcgtg agcgtcggca agaccgagga caagctaggc
     2221 cagagggcga gcaacacagc agtcatcaac ttcgacgatg ttcgggtttc gtctgacaac
     2281 ctgcttgccg agcagggcca cggtttcaag ctggccatgc agaccttcga ccgcacgcgg
     2341 cccgatatcg gtgccggggc ttgcggactc atgcggcgtg cgctcgagga gtcggtcgcg
     2401 tacgcgttgg agcgcaaaac gttcggagtt cccatcgcgg agcatcagat ggtccaggcc
     2461 atgatcgccg agatgggcat caagtacgag gctacccgcc tcttggtctg gaaggctgcg
     2521 tggcagatcg atcagggagg ccggaactcg ctcgtcgcct cgtacagcaa ggcttttgga
     2581 gccgatagcg cgatgcaggt ggctaccgat gcagtgcaag tcttcggtgg gaatggctac
     2641 atcaccgagt acccagtcga gaagctcatg cgcgacgcga agatccttca gatctacgag
     2701 ggcacctctc agatccaacg gatggtcatt gcaaagaacc tcttcgcggg cgtccgctag
     2761 cctcgttcta gcgacggttg ttgccggttg gctcagctcc accgctagcg cggagctcga
     2821 gccgcctccg ccgttcgtgg gctcgaatgt cgaccaagac gagctcctcg aggtgatctc
     2881 cactgcgaag tcgcggagct tcaagcccgt cggacacagc tctgtggttc ttcggatgcg
     2941 taccgttgct cacgtcacgg ccgggctcaa agagcgtagc aaggcgatcg aggatggcta
     3001 ccaatacgag atcgcagcct atcggatcag caggttgctc ggcctagaca acgtgccgcc
     3061 ggccgtttac aggcgcgtcg cctggcgtga ggtccaaaac cggttccacg aagacaagct
     3121 cgatcagcgc gcctcgatcc ggcgcaaggt gctttgggac gacgacggct cggcccctgc
     3181 cgctgccgtt tactgggtca aaggcatgaa atcgatcggt ctcgaaaagc gatcgacctg
     3241 ggagagctgg ctgcgagacg gagagattcc cgagtctcga gcggcactcg cacgtgacct
     3301 ctcgaccatg gtcgttttcg attttctcat cggcaactgg gatcgcttca gtggaggcaa
     3361 tctgccgatc gattcgagcc gggagcgggc catactacga gacaacgatc gctcattttc
     3421 gaatccgctg cacgagcgac gctacgaaat gctgctcgac ggattgaccc gaacgagtcg
     3481 attctcgaag aacctggttc gtcgcctcag cgagctcgac gaagcttcac tcagagccga
     3541 gctcgccgag gacccgagcc aggcgacgca gccgctgctg aacgacgccc agatcgcgga
     3601 cttcctggag aggcgggcca cggttctttc gtacatcgca gccatgatcg aagaccgcgg
     3661 cgagcctcaa gtgctcttct ttccttgagt attgccccgt ggtaggaaaa ggcgtggcca
     3721 agcccaaagt gatcgatccg gacgaatggg ggcacgtgtt gggtctgctc gttcatgatc
     3781 tgcgaaaccc cgcggccacg attacggcca acgtcgactt ccttcaagag gtcgggttgg
     3841 tcgacacgga ctcagtggag gctttgcagg atgtgaagct agccgttggg gagctgaggc
     3901 ggggtctcga catgctcgct tggatcagcc tctggctcag cggtcaggtt ccgctcgagg
     3961 ctgcaaatgg cgacgtaggc atgttcctag agcggctccg acgagtcgat acaccggtgc
     4021 cagtttccgt cgagatcgca gagggccagc agctttacgc ccagggcgcg caggctgcgg
     4081 tcgaggtggt cgagcttcta cttcacaaca cacgcgccaa tgtggccgac cctcgtgcga
     4141 ccgttcgcgt ctttcagcaa ggtggccatg tcgtgatcga ggtcgaggac gctggggaag
     4201 ccatagcgcc ggagctccgg gacggcgcat tctcgctcga tggtcagagg gccttgaagg
     4261 gtcgcctcga cgggcgctac agccgtttcg tagggctgtt cgccgcagcg gtggtcctcg
     4321 acggagtcgg tggcaccatc gaggcagacg gcgagaaagg cgcggcgatc ttcaggattt
     4381 tccttcgggg ggctcgcccg actcgtccgc ctcctcgcta gagcgctcac caagctcgcg
     4441 cagccgttct cgaatgcgcg cttcgaatcc tcgtgtcgtg ggccgatagt atgtccggcc
     4501 gacgagggcg tcgggcaggt acgtctcacc agtggaaaag ccgctttcgt cgtgagggta
     4561 tcgataaccg tctccgtagc cccagctctt catggctccg gtggacgcgt ttcgaagctt
     4621 gaggggaacg ggaagcgctc cgagctcgcg cacgtcggtt tgcgccgccg tccaggcctt
     4681 gtagctagcg ttgctctttg gtgcgctggc taggtagata cagcattgcg cgatcggaaa
     4741 gatgccttct ggcattccga gccgacgaaa cgccgcgtct gagctcaccg cgagatcgag
     4801 cgctctaggg tccgcgttac cgatgtcctc gcttgcgaaa atcaacatgc ggcgaagaac
     4861 gaagagcgga tcctcaccgg cttcgagcat tcgcatcaac cagtagaccg aggcgttcgg
     4921 gtcactgccg cgcatcgatt tgatgaacgc gctgatgacg ttgtaatgct cttcaccgga
     4981 cttgtcgtag agcaacgtct gcgttgcgag tgcctcttgg acgattccca ctgtgaccgt
     5041 ttcgactccg gccgactgag cgaagtcgct cgcgagctca agcgccgata gagcgcgccg
     5101 agcgtcgcct ccagcgtttt gtgagatggc agcgagcgcg tccgcttcga aacgatgagg
     5161 cacgtgtcca acaccatgct cttcgtcccc tagggcgcgc tcgagcaagc ggtgcaagtc
     5221 ggaagcctcc agcggtttga ggtcgaacac gcgcgcccgt gatagcagcg ccgagttgac
     5281 ggcaaacgat gggttctccg tcgttgcgcc aatcagtgtc accgttccgt cttcgacgtg
     5341 tggtagtaag gcatcctgct gggccttgtt gaagcggtgg atctcgtcga tgaacaagat
     5401 cgacttcttt ccgtaatagc tcttctgttc cttcgccgcg gcgatcagct tccgaagctc
     5461 cggcacgcca ccgagcaccg cgctgaatgg cacgaaaatc gaatgggtct cgtacgcgat
     5521 gacgtgtgcg agcgtggtct ttccggtacc aggaggaccc cagaggatca tcgatggaat
     5581 gcggtcggca cgaatcgctc gcgccagtat gtgtccgtcg cctagcagat gcgcttgccc
     5641 gaccacgtcc tcgagccgct tcggccgcat tcgatcggcc agcggggagc tcgattccgc
     5701 ctgtcgctca aagaggtcca cttcgagcct tataactcag cgcaggatgc gtagtctctt
     5761 cggaccttca ccctcggggg cggattctcc tccgccccgg tcgaagtgct ccttggctat
     5821 ccgctcggct agggcgtccg caacgccggc aaaagcctgg gcggcagccg aggcaggggc
     5881 tgcctggacg atgggcgtgc cctgatcgcc ccattcacga accaaggtgt cgatgggcac
     5941 ctggccgaac agcggcgcct cggcgaactc ggcgatcttc gcgccgcctc cactgccgaa
     6001 gatctcgtgc ttcacgcccg cgctgtcgat gaagtgactc atgttctcga cgacgccgag
     6061 gatcggaaca ttgagcttct tgcacatgga aaccgatttg tagacgtctt gcagcgcgac
     6121 ttcctgcggc gtcgtgacga tgatcgcacc agtgatgcgg agcttctgtg cgagcgtgag
     6181 tgccacatcg cccgttcctg gcggcgaatc gatgatgagg taatcgagcc caccccaatc
     6241 gacgtcactc acgaactgct gaagcgcccc atggagcatc ggacctcgcc agacgactgc
     6301 ctgcttcgga tcctccagga ggaagccaat gctcatcagc ttgattccaa agcgctgaag
     6361 cggctgaatc cgctttccgt ctttggacgt gggctgccct tcgatgccca gcatggtcgg
     6421 cagcgacggg ccgtagatgt ccgcgtcgag aaggcctacg cgggttccca ggcgacggag
     6481 tgccaaggcg agattcgtgg cgaccgtgct cttacctaca ccgcccttgc cactcatcac
     6541 caggatgacg tttcggacat ctggaatcgg atcggtgctg gtcgcttcgc gcgtgggaac
     6601 gttgacctcg agctcgagct cggtgaactc gatgccgagc ggctcgagcg ctgccttcac
     6661 cctcgcatgg agcgttcgcc gcacttcttc ggacggagag ctcactcgca cctttgccca
     6721 gacgcgcccg tcttcgaggc gcgcatcgat gagcgttccc aaggctccga gcggcttctg
     6781 aaagataggg tcttcgactt tgcgtagcgc gtcagtgact gtgtcgaggg taggggaccc
     6841 catcgaaacc tccaggtcgg ttcaaccaaa gggctctagg gcggcggttt atccgcgtca
     6901 agttggacaa acttcagttc gctcttggcg gcggttatgc tcccgtcgtg tcgatctggg
     6961 agcgaagctg ggcggttccg ctcgcgatct ttctactctg ctcgggcgtc tactgcgcga
     7021 ccgcgggtgg gcggctgttc cgaccttccc ccaacaatca ctacgtgcac ttggcagatg
     7081 cctggttgca cggccgcctc gacctcggcg cgcacgcacc aggaaccaac gactgggcgt
     7141 gtttcgacac ccaggaccgc ggtccgtgtc cgtcggggcg gtattcgttc tctccgcccg
     7201 agtcgacccg ctaccgttgg tacgtctcct ttcctccact gccggcgatg atactgctgc
     7261 cgctggtcgc ggtcttcggc ctgggcacat tggacgcgct cttctgggct gtcttcgccg
     7321 gtctcgcgcc catgtttcta ttcaccgtcc tgcgctacct gcgcgaatcg ggacgcagcc
     7381 accgttcact ccgtgacgat ctgatcatca ccgcgttgtt tgcagtgggc tcggtctact
     7441 acttcgttgc agtgcaggga acggtttggt ttgctgcgca ggtcgtcgcc tcggcattca
     7501 tctgcctcta cttgctggcc tcgtttgggg cgcgtcgacc cgcattggca ggactggctc
     7561 tcggcctcgc gttcctcagc cgaccgtcga ctctgctcct cggtgcattc ttcgtgcttc
     7621 aagcgatctc tgccgcgaac gaaagaggac aaccgagccg gagggccagg gctcagctca
     7681 tcggcaaact aatcaagttc gcgctgcccc tgaccttttt gctcggagcg gccatgtggc
     7741 acaacgccgc aagattcgat gaccccttcg agttcggtca ccgattcctt caggttcgct
     7801 ggcgttccag gattgaaacc tggggtttgt tcgggacgca ctacctcccg agaaatctga
     7861 aggtcttctt tctctccttg ccctggatcc tcgaatccaa tcccttcgtc cgcatcacgc
     7921 ggcatggact ggcgttctgg ttcacgacgc caaacctcgt ctggagcctc ttcccgaaga
     7981 agctcgattc gacgatcgtc gcgctttggg cagcggttct gcccaccgcg atctgcacct
     8041 tgctctatca gaacacggga tgggttcagt tcggatatcg gttcgcgctc gactacctac
     8101 cgttcctatt cgtcctc
//
LOCUS       DASRZF010000322         5652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2659354, whole genome shotgun sequence.
ACCESSION   DASRZF010000322 DASRZF010000000
VERSION     DASRZF010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 5652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5652
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2659354"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VFG22_14145"
     CDS             complement(<1..244)
                     /locus_tag="VFG22_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417434.1"
                     /translation="MSFEQRYIGEILVRRGALEPDKLENALELAADRHVDLADFLVAT
                     HAVEEDKLVRALADEVDMEFLEKIATELIPEELIDTV"
     gene            complement(244..3009)
                     /gene="gspD"
                     /locus_tag="VFG22_14150"
     CDS             complement(244..3009)
                     /gene="gspD"
                     /locus_tag="VFG22_14150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HET6417435.1"
                     /translation="MISRKQLICLVVGSLLLSTEHAAIAQQDVAKPATPPARIPSSPL
                     PLRPAPKADPKAEVAEEEPPELENELPQFETGVEFQPMSPRTRVTFNLEDAELPDLVR
                     LISNMTGRRFILPTKLRAIKATVFAPTKVTVAEAYQAFLSVLEVNGFTVVPSGRYLKI
                     VETTNIEQQTIPLYEDGSPTPASDRYITRIHHLENVSAEDVTNLLARFKSASGNITSY
                     GPTNMLIITDTGAQIRRMLRLVAAIDLPRSGTQTWIEPIHYANAAELASRLLEIFPAD
                     TAAASPAAQKARPAPRAGSNANDTALVGDVAAESAIRNIIADERTNSLIIISTERAYL
                     RILEMIRQLDIALEGEGRIHVHYVQHGAATDIAGALTALVGASSGRPTPGGPRAQRAA
                     ATPVTTGTLFEGAIAVTAYEPANALVITSSLHDYTALRNVIERLDAPRKQVFIEAVVM
                     ELGIERSSELGFSFHGGAGNFPTDGSLSLFGFNAPSSIDITQNNLTGLALGVRGPTIE
                     NSQQLVGFSVPGFGVVVTALAQSGDADVLSTPHIIAIDNTEAEISVGENIPLQTNGVA
                     PGTFAGAGSLGSLAQAGAAGADLGALGGLAGGLGSVARQDVGTTIRVTPHINANNEVR
                     LEIEEEISEQGATSGTLGVVSINRRTARTEVMVRDQQTIVIGGLMRDAIQNKEDKVPV
                     LGDIPILGALFRKTSKQKRKTNLLLILTPYIIRDPGDLREIFERKMQERQQMIDRYFV
                     FGEDKFEPHIDYSRTRGLVAEIVNEIDGVEEEILLAKAAELEPPPDHVPRAPIGSYGA
                     PELREEETVVIGPNGVEEMETEEPGSTISAPPTAADEQEGGSAPPLPIEDADDAEGAE
                     DQAPSEPGAPEEPTAPGGPEEVIEPDGPAEGPAETVDANEPGSLPLPAPVVEEPPPAT
                     RDVEGLE"
     gene            complement(3045..4028)
                     /gene="gspC"
                     /locus_tag="VFG22_14155"
     CDS             complement(3045..4028)
                     /gene="gspC"
                     /locus_tag="VFG22_14155"
                     /inference="COORDINATES: protein motif:HMM:NF041515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HET6417436.1"
                     /translation="MATARENRFSMAILLATLAACAFFLAQGTTRVLAAELLAVDGGP
                     AAPTGRRSTPASSEFPRKRDAAIILRRNIFDSTRGDLTAEPIPEVAIGADGEPLEEWD
                     PSQPPAKCTSKLRLVGSVMSPGYPDWSFAAIAGSSGGKTMLYREGSTVDGSRILAVYP
                     SSVVVSSSSGACQLLMFEEEEDAGSVRTPAIASRPAPSTPANARNAGLSDAELEDGIE
                     KISDTKFSIQRSLVDKALANQGSLMRAARVIPHEENGRVVGVKLYGIRRNSLLGRLGV
                     RNGDMLRTINGFSMTSPDTALEAYSRLRTADKLSLAIKRQNNEMTIDYNIE"
     gene            complement(4185..5543)
                     /locus_tag="VFG22_14160"
     CDS             complement(4185..5543)
                     /locus_tag="VFG22_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6417437.1"
                     /translation="MVDSNQISVLVVDDEAPNIDSLRRIFEREGFSVLTATSGQKALD
                     VCRRNAVHVVVSDLMMPGMNGIELVKALKTVVPDAEVVLMTAFGTVEKAVEAMRAGAY
                     DFVEKPLKRIQMVKTVRKAAERHQLVVENKDLRQQLSQLSDRSIVGSAASLRAALEIA
                     TQAAPSSANVLILGESGTGKELLARFIHERSGRSRFVGVNLSALPETIVESELFGHEK
                     GAFTGAIARRDGRIAEAEGGTLFLDEIGELSAAVQVKLLRVLQEGEYQPVGGHTKQAD
                     FRLIAATNRDLRAAVETGEFREDLYYRLNVIALTSPPLRARRDDIPLLAEHFVETYCK
                     KNDKDPLAIERDAVQQLVAYDWPGNVRELQNVIERAVVLNKSGVIKLADLPDPVARAE
                     PQLDAFAFPVGTPLSEVERGVIHGTLKHTDGDKQLAAQLLGISARTIYRKLGAEKGDE
                     LG"
BASE COUNT          979 a         1728 c         1736 g         1209 t
ORIGIN      
        1 gcaccgtatc gatgagctcc tctggaatga gctcggtggc gatcttctcc aggaactcca
       61 tgtcgacttc gtccgccagc gcgcgaacga gcttgtcttc ctccaccgca tgggtggcga
      121 ccaagaagtc ggcgagatcg acgtgccggt cggccgccaa ttcgagcgcg ttctcgagct
      181 tgtccggctc cagcgcacca cgccgaacga ggatttcacc gatatagcgc tgctcgaagc
      241 tcattattcc aagccctcca catctcgggt cgccggcggc ggttcctcaa cgacgggggc
      301 cggcaaggga aggctaccgg gctcgttcgc gtcgacggtt tccgcagggc cctcggcggg
      361 cccgtcgggc tcgatcactt cctccggacc gcccggtgcc gtcggctctt ccggcgctcc
      421 cggctcggat ggggcttggt cttccgcacc ttccgcgtcg tccgcatctt ctatcgggag
      481 aggtggcgct gagccgcctt cctgttcgtc ggcagccgtt ggcggcgctg agattgtaga
      541 acccggctcc tcagtctcca tctcttcgac gccgtttgga ccgattacga cggtctcttc
      601 ttctctcagc tcgggcgctc cgtacgagcc gatcggggca cgcggaacgt gatccggcgg
      661 aggctcgagc tccgcagcct tggccagaag gatctcttct tccactccat cgatttcgtt
      721 gacgatctcc gcgactaggc ctcgcgttcg cgagtagtcg atatggggtt cgaacttgtc
      781 ttctccgaag acgaagtacc gatcgatcat ctgctggcgc tcctgcatct tgcgctcgaa
      841 gatctcacgc aggtcgcctg ggtctcggat gatgtacggg gtgaggatga ggaggaggtt
      901 ggtcttgcgc ttttgcttgc tggtctttcg gaagagcgca ccgaggatcg gaatgtcgcc
      961 aagcaccgga actttgtctt ccttgttctg gatggcatcg cgcatgaggc ctccgatgac
     1021 gatcgtctgc tgatcgcgca ccatcacttc cgtgcgcgcg gtgcgccggt tgatcgaaac
     1081 aacgcctaag gttccagagg ttgcaccctg ctccgagatt tcctcttcga tctccagtct
     1141 cacttcgttg tttgcgttga tgtgcggtgt gactcggatc gtggtgccca cgtcttggcg
     1201 tgcgacgctt ccgagaccgc cggccagacc ccccagggcg ccgagatccg ccccagctgc
     1261 acccgcctgg gccaaggagc ccaagctacc cgcacccgcg aaggtcccag gcgccacgcc
     1321 gttggtttgc agcggaatgt tctcacccac gctgatctca gcctcggtat tgtcgatcgc
     1381 gatgatgtgc ggcgtcgaaa gcacgtcggc atcgccggat tgcgccagcg ccgtcacgac
     1441 gacaccgaat cccgggaccg agaaaccgac gagctgctgc gagttctcga tcgtcggtcc
     1501 tcgaacacca agcgcaagac ccgtcaaatt gttctgcgtg atgtcgatgg atgaaggagc
     1561 attgaatccg aatagagaaa gcgaaccgtc ggtcgggaag ttccccgcgc ctccgtggaa
     1621 cgagaatccg agctccgatg agcgctcgat gccgagctcc atgacgaccg cttcgatgaa
     1681 tacctgcttg cggggcgcat cgaggcgctc gatcacgttt ctcaacgcgg tgtagtcatg
     1741 aagcgaggat gttatgacga gtgcattggc tggctcgtac gcggtcacgg caattgcgcc
     1801 ttcgaagagc gttcccgtcg tcacaggcgt cgccgcggca cgctgcgccc ttgggccccc
     1861 gggcgttggt cgtccggacg aagcgccgac gagtgccgtg agcgctcctg cgatgtcggt
     1921 tgcggcaccg tgctggacgt agtgtacgtg gatgcgtccc tcgccttcga gggctatgtc
     1981 gagctgacga atcatttcca agatgcgtag gtacgctcgc tcggtggaaa tgatgatgag
     2041 ggagttggta cgctcatcgg cgatgatatt tcgaatggcg gactcggctg ccacgtcgcc
     2101 gacgagtgcg gtgtcgtttg cattactgcc cgccctcggc gccggtcgtg ccttttgggc
     2161 ggccggcgat gccgcagcgg tgtccgccgg aaagatctcc aacaagcggg aagcgagctc
     2221 cgcggcgttc gcatagtgga ttggttcgat ccaagtttgc gtaccgctcc gcggcaggtc
     2281 gatcgcggca accaggcgca gcatgcgacg gatttgagcc cccgtatcgg tgatgatgag
     2341 catgttcgtc gggccgtacg aggtgatgtt tcccgaggca gacttgaagc gtgcgagaag
     2401 gttggtgacg tcttccgcag atacgttttc gagatgatga atccgcgtga tgtagcgatc
     2461 ggatgcgggc gtcggactgc catcctcgta gaggggaatg gtctgctgtt cgatgttcgt
     2521 cgtttcaacg atcttcaggt accggccgga ggggaccacc gtaaacccat tgacttcgag
     2581 cacggatagg aatgcctggt acgcctctgc gacggttacc ttggtcggcg cgaataccgt
     2641 ggctttgatc gcacgaagct tcgtcggaag gatgaagcgc cgccccgtca tgtttgaaat
     2701 caggcgcacc agatccggca gctccgcatc ttcgagattg aacgtcactc gggtcctcgg
     2761 cgacatcggc tggaactcga cgcccgtctc gaactgcggc aactcgttct cgagctcagg
     2821 gggttcttct tcggcaacct cagctttggg atctgccttc ggagccggcc gcaagggcaa
     2881 tggcgagctc ggaatccgtg ccggaggagt tgctggcttg gctacgtctt gttgagctat
     2941 cgcagcgtgt tccgtgctca gcagcagcga gccaacgacc aagcaaatta gctgttttct
     3001 agaaatcacg attcattccc cgcctgagtt cccaaggtgg cgcatcattc aatgttgtag
     3061 tcgatggtca tttcgttgtt ctgccgcttt atcgccagcg agagcttgtc cgccgttcgc
     3121 agacgcgaat aggcttcgag ggccgtgtcc gggctggtca tgctgaatcc gttgatcgtg
     3181 cggagcatgt cgccattccg aacgcccagc cgtccgagca gactattgcg gcgaatcccg
     3241 tagagcttga cgccgaccac tctaccgttc tcttcgtgcg gtatgactcg cgctgcccgc
     3301 atcaagcttc cctgattggc cagggcctta tcgaccaagc tccgctggat gctgaacttg
     3361 gtgtcgctta tcttttcgat gccatcctcg agctcggcgt cgctcagtcc cgcgttgcgt
     3421 gcgttcgctg gagtgctcgg agcgggcctg ctcgcgatgg ccggggtgcg gaccgagccc
     3481 gcgtcttctt cttcttcgaa catcagcagt tggcaggctc cactcgagct gctgaccacc
     3541 acgctcgaag ggtagaccgc gagtatgcga gagccgtcca cggtcgatcc ctcgcgatag
     3601 agcatcgtct tgcctccact cgaacccgcg atcgcggcga aggaccaatc cggatagccc
     3661 gggctcatca ccgagccgac gaggcgcagc ttgctcgtgc acttggcggg aggctggctc
     3721 ggatcccatt cctcgagtgg ctccccatcg gcgccgatcg cgacctccgg aattggctca
     3781 gcggtgaggt cgccgcgcgt cgagtcgaag atgttccttc gaagaatgat cgcagcgtcg
     3841 cgcttgcgtg gaaactcgct ggaagccggc gtgctccgcc tgccggtcgg cgcggccggc
     3901 cccccatcga ccgccagcaa ctcagccgcc agtacgcggg tcgtcccctg ggccaggaag
     3961 aaagcgcagg ccgccagcgt tgcgaggaga atcgccatcg agaatcggtt ttcgcgcgca
     4021 gttgccaccg aggttcacta cagcaaccac cgtgccgaag cgaattgtcg gaaagaccaa
     4081 tcatttcgtg actggaggca gagtgcggag ctcgtctcat ggacaagctg gcggtcctga
     4141 agtgcctcga ttgacaaaat gacagaagag cagggcgcgg ctgttcagcc gagctcgtcg
     4201 cccttctcgg cgccgagctt gcgatagatc gtacgcgctg aaattccgag gagttgcgcc
     4261 gcgagttgtt tgtcgccgtc ggtgtgtttc agcgtgccgt ggatcacgcc tcgttccacc
     4321 tcgctgaggg gcgtgccgac tggaaaagcg aaagcgtcga gttgaggttc tgccctggcg
     4381 accggatcgg gtagatccgc gagcttgatc acgccgctct tgttcaacac aaccgcgcgc
     4441 tcgatcacgt tctgcagctc ccgcacgttg cccggccagt cataggccac gagttgttga
     4501 acggcgtcgc gctcgatggc gagtgggtcc ttgtcgttct ttttgcagta ggtttcgacg
     4561 aagtgctcgg ccagaagcgg aatgtcatcg cgtcgtgctc gcagcggcgg gctcgtcagc
     4621 gcgatgacgt tgagccggta gtagagatct tcgcgaaact cgccagtttc caccgcggct
     4681 cgaaggtcgc gattggtggc tgcgatcaat cgaaaatcgg cctgcttcgt gtgcccaccg
     4741 accggctgat attccccttc ctgtagaacc cggagtagct tcacctgcac ggccgcagag
     4801 agctcaccga tctcgtcgag gaacaaagtg ccgccctctg cctcggcgat gcggccgtcc
     4861 cgacgtgcga tggcgcccgt aaaagcgcct ttttcatggc cgaaaagctc cgactcgacg
     4921 atcgtctccg gtaacgcgga aaggttgacg ccgacgaacc ggctcctgcc gctgcgttcg
     4981 tggatgaaac gcgcaaggag ctccttgccc gttccgctct ccccgagaat gagcacgttt
     5041 gccgatgatg gggccgcctg cgttgcaatt tcgagggcgg cgcgaagtga tgctgccgag
     5101 ccgacgatgc ttcggtcgct cagctgggac agttgctgcc gaaggtcttt gttctcgacc
     5161 acgagttgat gacgttcggc tgccttccgc acagtcttga ccatctggat tcgcttgagc
     5221 ggcttctcga cgaagtcgta tgcgccggct cgcatcgctt ccaccgcctt ttcgacggtc
     5281 ccaaacgcgg tcatcaagac gacctccgcg tccggaacga cagtcttgag cgctttgacc
     5341 agctcgatgc cgttcatgcc gggcatcatc aggtcgctca cgacgacgtg aacggcgttg
     5401 cgccggcata catcgagggc cttctggcca ctcgttgcgg tcagcacgct gaacccctct
     5461 ctctcgaaga ttcgccggag cgagtcgatg ttgggcgcct cgtcgtcgac gacgagaacc
     5521 gaaatctggt ttgaatcgac catgggagac tccgtgacaa tcaactttgc cactatgtca
     5581 ggcgatgaca agtcgtccaa actttctgac gccatgtaat tagctgtaat cactgagttt
     5641 atctccgtaa aa
//
LOCUS       DASRZF010000323        16725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_249729, whole genome shotgun sequence.
ACCESSION   DASRZF010000323 DASRZF010000000
VERSION     DASRZF010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 16725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16725
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249729"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1626)
                     /gene="recN"
                     /locus_tag="VFG22_14165"
     CDS             complement(<1..1626)
                     /gene="recN"
                     /locus_tag="VFG22_14165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HET6417438.1"
                     /translation="MLTCLRVRSFAIIEALEVELDAGLNIVTGETGAGKSILVDALQL
                     VLGARGRPEVVRTDADAAEVEALFDLSGQAEVLQSLRDQGVETDGELLIRRVVTSTGR
                     TRAYVNGRLVTLAQLKEVTKGLADISSQHEYHSLADARHHLSYLDAFANHGALLDEVG
                     QAHATLSEAAARLEQVVTEERGKADREDLLSYQVNEIESLREALESEEALLVERERLR
                     HAEKLGIASGEAEAQLYSDDDSICGAVARIAGSLEQASAFDPSLETIANQVREALLQL
                     EDAASELGSYAQDLSFDPARLGTLEDELDRLSRVKRKYGSTGEEVLAYRDQAAAELDA
                     LVRYEERLDRHQRELDEALEEASRVALTLRTKRKAAAAKLSKAISKELSSLGMGEAKV
                     TVELAALEGGPRELQVHGARLSPTGIDHAELLIAPNRGEEAKPLRKIASGGELSRAML
                     AIKRVLAGIGPASLYVFDEVDAGVGGAVAEVIGRKIHEVAQHSQVLCITHLPQISAYA
                     DAHYRVHKEVVGKRTKSDIRLLTKNERLEEIARM"
     gene            1687..4350
                     /gene="alaS"
                     /locus_tag="VFG22_14170"
     CDS             1687..4350
                     /gene="alaS"
                     /locus_tag="VFG22_14170"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HET6417439.1"
                     /translation="MASSSEIRESFLRFFAEREHERVASGPLVPPNDPTLMFANAGMV
                     QFKDVFTGKEQRPYARATSSQKCIRISGKHNDLENVGVTARHHTFFEMLGNFSFGNYF
                     KRDAIKFAWDYFIDTLELDPERLVVTVFGGEEGFAPADDEAAAIWKDVTGFPNERIIR
                     CGAKDNFWQMGDTGPCGPCSEIHYCLGLDDVDPRRFGEEPAADGRGWFELWNLVFMQF
                     ERHAGGKMDLLPAPSIDTGAGLERIAVVKQGVLSNYDTDLLRPIVDLAAEISGKRYGE
                     SQSHDDVSMRVIADHARTTAFLISEGVFPDKDGRAYVLRRVMRRAIRHGHRLGIGEPF
                     FHHCALRVVDDMGDVYPQLVERRALIEEITQQEEERFRRTLDRGLQLISENHEWIESN
                     GARSMPGAVAFKLYDTYGFPLDLQNVIGEEQGFTIDQDGFDRELGEARLRSQGSKVGS
                     SAVAEIYPALVQEVGAVEFLGYDSEVARSEVLALVQSGDSVKELGSGASGEIVTRATP
                     FYPEQGGQVGDRGVIELAGARFEVEDTQKPVEGLIVHRGKVISGSFQPGAEVELRVDH
                     GLRSATRRNHSATHLLHWALRQVVGATAAQKGSLVGPERLRFDYSGTRPLEAAEIERI
                     EDLVNARVLENTDITTEALAMDAAKERGAIGIFEEKYGDVVRMLTIGPSLELCGGTHA
                     ARTGDIGLFKVLSETGLAAGVRRIEATTGLNALRHLRRLDSELSGAAALLKAPAQQLR
                     DRVERLIEQQKESQREIDRLKRSLMSGDSADLSADAREVDGARVLGAVVELGDAGALR
                     EMADQLRDKLAPAVILLGSPSKDGKKALLVCSVSKGLTKRFRAGDIVKAAASIVGGNG
                     GGRPDFAQAGGPDATKIEDAVRQVYELTGVA"
     gene            4351..5655
                     /locus_tag="VFG22_14175"
     CDS             4351..5655
                     /locus_tag="VFG22_14175"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HET6417440.1"
                     /translation="MSAKSKLVGAFLNVYLNSRPAKHALHRAGRVLRGGRRRARLYYR
                     ADDPYSHLLAQVAPRLRDAYGLEIQIIPVAHPSMAANPAPEMLLEHAIQDAAILAESY
                     GLSFPLAAQAPAEDRVRRAHAVLLERRPADEQVDVAIELGEAVWRNDGTSLAAIVDRH
                     GSLSGESVRPALEANYTALERTGHYQPGMLYYDGDWYWGIDRLHYLEERLQRERLEGN
                     ISLTRGSTPELARLLRRPEGSRPGMEAFLSFRSPYSYLAVPQLIQLRDRYGVDVDVRP
                     VLPMVMRGLPVSLAKRLYIVHDAKREADRLGIPFGRVSDPLGEGIGYCMAVFFNCAVD
                     KGLGLEFARSVMQGIWAEARDVAHVPDLLFLAERAGIAEAEVMAALETGNWEQRAQAN
                     REALTEMGLWGVPSFRIGEYSTWGQDRISFIESEIARFSAPA"
     gene            5713..6150
                     /locus_tag="VFG22_14180"
     CDS             5713..6150
                     /locus_tag="VFG22_14180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417441.1"
                     /translation="MTVSEEKLAGADQALAELGRSEAEIAEVRGRFANAAAVDLEATD
                     AILSTLAEGVSVELTSSEARASVAQSVEDVWDDEHTEVEIIDESDFVLLVDENELEEL
                     EKVGEDDAVRSLPPALPQEGADGEEGDGFFKKLFGNRRSSNRP"
     gene            complement(6166..6855)
                     /locus_tag="VFG22_14185"
     CDS             complement(6166..6855)
                     /locus_tag="VFG22_14185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417442.1"
                     /translation="MIRFVLLPSLAFALVFGSTEAARAEVVVVQPNDQSSPVIVKTKP
                     PTVVVVERQSIPQPQAQAPTPPKRDRKLGLHFDVGGTFGPQVLMGGLSGALRFRPKEH
                     VAIDLGSGYFAGNDYQGFYRTEIPLTVNVLFFVNPQHKAQVYFLLGPGVSFGRVDTYD
                     ELRHMTYIGGQAGMGLELRLAPAFALNVDVRGVLRHRIDNDPRPEFFDGTRSTDTSGG
                     AVLAFGATFYF"
     gene            complement(6952..7551)
                     /gene="recR"
                     /locus_tag="VFG22_14190"
     CDS             complement(6952..7551)
                     /gene="recR"
                     /locus_tag="VFG22_14190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HET6417443.1"
                     /translation="MTANDPIASLISLLSRLPGIGTRTAARLAFHVLEGEQQYAEALG
                     EALRGIHDQVTRCELCGNYGAESRCAICRDPRRDASLICVVAKVPDLIALEALGSFRG
                     RYHVLHGLLAPLDGIGPNQLPLDALERRIAEEGVNEVIVATPLNVEGEATALYVADAL
                     SASAVRVSRIAAGVPHGGELEFTDQVTLGRALEDRRAVR"
     gene            complement(7555..7875)
                     /locus_tag="VFG22_14195"
     CDS             complement(7555..7875)
                     /locus_tag="VFG22_14195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HET6417444.1"
                     /translation="MNFRGGMGELMRQAGRVQRKVEKRREELREETFEARAGNDKVTA
                     IANGIPELAEIRIDKTLLEEEDIEMVQDLVVAAANAALTKAREHVDAEIEKITGGLSI
                     PGIG"
     gene            complement(7885..9681)
                     /gene="dnaX"
                     /locus_tag="VFG22_14200"
     CDS             complement(7885..9681)
                     /gene="dnaX"
                     /locus_tag="VFG22_14200"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HET6417445.1"
                     /translation="MAYTVLARKYRPQSFEDLVGQEHVARTLANAIESDRVAHAFLFT
                     GVRGVGKTTTARLLAKALNCENGPTPTPCNDCGPCRDITAGIDVDVQEIDGASNNSVE
                     DVRRLQESLPYRPQRDRYKIVIVDEVHMLSAGAFNAFLKTLEEPPDHVKFIFATTESH
                     KVPITIRSRCQQYDFRLIPHRLIAERVREILETESLSADDEAISVVAREAAGSMRDAL
                     TLLDQLVAISDARITADTVAASLGIAAREALYEVASAVLEGDGARVVKAVDGLADKSV
                     DMQHFARQLLQLIRDLVVLKLDAGDPADWVPEERETAMQLIEDVDILELQRAFRAASI
                     VMDEMAQSPNPKLMLEMGLVRVATRPPLQSVAELIARLDGLQGKPGPSGGSPGGSRAR
                     TTKPKPEPAPAKPKAEATPAPKPKVEVDAAPRSASDPEPKPQNPNQPVMPATKHAGGF
                     QTSTGEVLTPIRRAALQEWEELVGTLEQQQPALAAVLEHGIPKTVGPDRIVLSFKDGS
                     FYGRQAGSPESIAAIVRIAEQQLGGRPEIEIRFDAQGEAATKTVAAVAAQRREAELDA
                     KRKEALNHPRVRDAVDVFPESAGKLKVLIEAD"
     gene            complement(9771..11354)
                     /locus_tag="VFG22_14205"
     CDS             complement(9771..11354)
                     /locus_tag="VFG22_14205"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HET6417446.1"
                     /translation="MNAVRERKLPFLIMVTIIVFLPFLGKGGILASAIIAAALVGSPL
                     FVLIGVATLGSLFLWSGFTELNQFTIVIEGIRSLADSPTLLAIPFFIMSGAVMSKGQI
                     SQRLIEFAQAFIGWVPGGMAMSGVLACMLFAAISGSSPATVVAIGSMMGPALIAEGYR
                     ERFSHGLLTSAGSLGILIPPSIPMIVYPIVNQGAVIEVERLFASGFGPGLVMGSILIG
                     YSFYQGIVDKIPTSPFKFQKLIAASRDGFWALMFPLLILGGIYGGIFNAVEAAAVSVM
                     YAIVVEVWLHRALKLKAIPAIFGETGVFLGSFLVIMVMALALGEFLEKEEIPARMVEW
                     IQGKDLEYWQFLVLLNLLLLAVGMMMDILSAMFVFVPLLAPIAASMGVDPMHFGIIFI
                     VNLEIGYLTPPVGLNLFVASALYEKGLGHIIRSVAPFVGLMLIGLGIITWYPQVSIGL
                     GNAIMGADPNALLDEDGDGVDDRGEDAGGMPTMEEMMQEAMEADDDGDDDEDLDDDED
                     FDDDEDLGDDEDLGDDDVE"
     gene            complement(11351..12358)
                     /locus_tag="VFG22_14210"
     CDS             complement(11351..12358)
                     /locus_tag="VFG22_14210"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HET6417447.1"
                     /translation="MAEPGKENPYEGRFGIIRRIDDWIFHVEMGVLWTFLGASAIMVF
                     LDVVYRRLAAPDSKVAELCARIFGVETPEGMKTLTTIGPTATAVVGIGLVYFAFWTAE
                     NHAAEPGKRSRTKPIVEAIVACSGLGLLGWIMLQPTVESRWFYLLLYGLCALAWLVQL
                     LRQRAAGWVSKLIAFFVVTALLVYIIVNYFPDGYSWSKELSLIMLLWVGFLGASVCAH
                     EGKHIQVGALKRVVPPSLSKWTDAIGYITTAGFCFFMAALGYVYAKEALQLEGRFEQT
                     NIPDWVATIAVPAAFAMTTIRYVAAAFSSIMGGSYGAAPEDEALALAEQAKAAKQGAE
                     G"
     gene            12427..13605
                     /gene="dinB"
                     /locus_tag="VFG22_14215"
     CDS             12427..13605
                     /gene="dinB"
                     /locus_tag="VFG22_14215"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HET6417448.1"
                     /translation="MRTVIHFDMDAFFASVEQLDDPDLRGKPVLVGARSRRGVVAAAS
                     YEARPSGVRSAMSMVEALRRCPDAIVVPPRRSRYSELSTKVFQVFRRYTPLVEGLSLD
                     EAFLDVTASRSLFGDGPEIARRIKKEVRAETGLSGSAGVAANKFVAKIASDLHKPDGL
                     TIVPADASAEFLDPLPLERMWRVGPKARVRLRDAGLQTIGDLARTDVRTLERLLGTWG
                     PVARDLARGLDERPVVVGAPPKSLGSESTFEQDLRSCEALLKPILEQSMLLADRLVRK
                     GLWANVVTLKIKYGDHRIRTRQIRLARAVSDTDAIFEAATELLTRFDALGAGVRLTGV
                     SVSSLTKVPDAELFPDAERARRERLAATTGALRERFGTASLTRASLLASPDDIGDSHR
                     "
     gene            13725..15380
                     /locus_tag="VFG22_14220"
     CDS             13725..15380
                     /locus_tag="VFG22_14220"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HET6417449.1"
                     /translation="MNARHTKFIFVTGGVVSSIGKGLAAASMGALLEARGLRVTNMKL
                     DPYINVDPGTMSPFQHGEVFVTDDGAEADLDLGHYERFTKATMTKANNFTTGKIYDSV
                     ISKERRGEYLGATIQVIPHITDEIKARVREATRNVDVAIVEVGGTVGDIESLPFLEAV
                     RQLRGELGPQNSVSVHVTLIPHIAAAGELKTKPTQHSVKELLGLGIQPEILLCRVDRE
                     LPHALKEKIAHFCNVPVKAVIAAPDVRVIYELPLTLHEQGLDRLLAERLNIWSRDPDL
                     SVWRRTVERYLAPDRGHVSIALVGKYVHLRDSYKSLHESLVHAALECDVKLDLDYIDS
                     EELESGNVEARLSRHDAILVPGGFGDRGAEGKILAIQFARENDIPFFGICLGMQMAVV
                     EYARNVCGLQGANSAEFDPQAEHLVVDIMPDQRGVEDKGATMRLGAYPCTIKPDSLAL
                     QIYGTSRISERHRHRFEVNNAYRERLEDGGLVLSGVSPDGRLVEMVELPAHAHFIGCQ
                     FHPEFKSKPHTPHPLFTSFVRAAIEHHARRGKSVSGATEALVN"
     gene            15449..16384
                     /locus_tag="VFG22_14225"
     CDS             15449..16384
                     /locus_tag="VFG22_14225"
                     /inference="COORDINATES: protein motif:HMM:NF014789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6417450.1"
                     /translation="MNPRFPWGIPALMYGEQPPRSDLESVWDGYRQALQRGSSPFGAA
                     LEVASSVDRLGLAFAAGYSAALQTLVPDVVLPCALCVTEIEGSHPRAIKTTLRAAEVG
                     SSYALNGTKTFVTFGNLAKTLIIAARAGEHADGTPELVVVRIPTDRDGVSLIDLPPIS
                     FVPEVPHSRVELENVCVDPSERMSGDGYLEYVKPFRTVEDIHVYGSAVAYFFGLGKRT
                     GAPAGFLAEMVSILVALESLREATPLDPNTHVALHGVGEHLSHLITSDEFLGIWDAAP
                     PEERDRWERDRKLLDVAGSARRARFQKAATQLGLC"
     gene            16525..>16725
                     /locus_tag="VFG22_14230"
     CDS             16525..>16725
                     /locus_tag="VFG22_14230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417451.1"
                     /translation="MTRPARRKWLTIPTRIVLGYILVLVAFAVVGSLSFVQHHRTSQT
                     LGLLEDGYLPLALTLSQAKATQS"
BASE COUNT         3080 a         5179 c         5290 g         3176 t
ORIGIN      
        1 cattcgggca atctcttcta ggcgctcgtt cttcgtgagc agccggatgt cgctcttggt
       61 ccgtttgccg acgacttctt tgtgcacacg gtagtgcgcg tcggcgtagg cggagatctg
      121 aggaaggtgg gtgatgcaga gcacctggct gtgctgggcc acttcgtgaa tcttgcggcc
      181 gatcacttcg gcgacagcgc caccgacgcc ggcgtcgact tcgtcgaaca cgtagaggct
      241 cgcgggtccg atgcctgcca gcacgcgctt gatcgccaac atcgcgcgcg agagctcgcc
      301 accgctggcg atcttacgaa gcggtttggc ctcttcgccg cggttcgggg cgatcagcag
      361 ctctgcatgg tcgatgcccg tcggcgagag tcttgcacca tggacctgaa gctcgcgtgg
      421 accgccctcg agcgcggcca gctccacggt gactttcgcc tctcccatgc cgagcgagga
      481 aagctctttg ctgatcgcct tgctgagctt ggccgccgcg gccttccgct tcgttcgaag
      541 cgtcagagca acccgcgacg cctcttcgag tgcttcatcg agctcacgct ggtgccggtc
      601 gagacgttcc tcgtacctca ccaatgcgtc gagctcggca gccgcctggt cgcgataggc
      661 tagaacctct tcgcccgtgc tgccgtactt gcgcttgact cgtgacaagc ggtcgagctc
      721 gtcctcgagc gttccgagac gggcaggatc gaaagaaagg tcctgtgcgt agctacccag
      781 ctccgacgca gcgtcttcga gttgcagcag cgcttctcga acctgattcg cgatcgtctc
      841 gaggctggga tcgaaagccg aggcttgctc gagggaaccc gcgatccgcg caacggcacc
      901 gcagatcgaa tcatcatccg agtaaagctg tgcctcggct tctccggatg cgatcccgag
      961 cttctcggca tggcgaagcc gctcccgctc gactagcaag gcttcttcgc tttcgagcgc
     1021 ttctcgtagg gattcgatct cgttgacctg ataactaagc agatcctcac gatcggcctt
     1081 gccgcgctct tcggtgacga cctgctcgag ccttgccgca gcctcggaaa gagtcgcatg
     1141 tgcctgaccg acctcgtcga gcagcgctcc gtggttcgcg aaggcatcca gataggacag
     1201 atggtgccgc gcgtccgcca aggagtgata ctcgtgctgc gaagagatgt cggcgaggcc
     1261 tttcgtgacc tctttgagct gcgcgagcgt gaccagccgc ccgttgacgt aggctctcgt
     1321 tcgtcccgtc gatgtcacca cccggcgaat caagagctcc ccatcggttt cgacgccctg
     1381 gtcgcgcagg gactggagaa cctctgcttg cccgctcagg tcgaaaagcg cttcgacttc
     1441 tgcggcatcg gcgtccgtgc ggaccacttc gggccggcca cgcgctccca gcaccagctg
     1501 aagcgcatcg accaggatcg actttcccgc gccggtctca cccgtgacga tgttgagccc
     1561 ggcatcgagc tcgacctcga gcgcttcgat gatggcgaag ctccgcacgc gcaggcacgt
     1621 cagcacggcg caagctctag cacaggcgtt gcgcctgcgc tgcacaagcg ctagcaatcg
     1681 tcgaccatgg cctcttcatc ggaaattcgg gaatcgtttc tgcgattctt cgcggagcgc
     1741 gagcacgaac gcgtcgcgtc gggtccgctg gttccgccga atgatccgac tctcatgttc
     1801 gcgaacgcgg gcatggtgca gttcaaggac gtcttcaccg gcaaggaaca acggccctac
     1861 gcgcgtgcca cctcgtcgca gaagtgcatt cggatcagcg gcaagcacaa cgatctcgag
     1921 aacgtcggcg tgaccgcccg gcaccacaca ttcttcgaaa tgctcgggaa cttctcgttt
     1981 gggaactact tcaaacgcga cgccatcaag ttcgcttggg actatttcat cgacaccctc
     2041 gagctcgacc ccgagcgtct cgtcgttact gtattcggtg gcgaggaagg gtttgctcct
     2101 gcggatgacg aggccgcagc gatttggaaa gacgtcacgg gctttccgaa cgagcgcatc
     2161 atccgctgcg gcgcaaagga caacttctgg cagatgggcg atacaggtcc ttgcggcccc
     2221 tgctccgaga ttcactactg cctcgggctc gacgacgtcg acccgcgcag attcggcgaa
     2281 gagcctgcag ccgacggccg cggttggttc gagctgtgga acctcgtctt catgcagttc
     2341 gagcgtcatg ccggcggcaa gatggatctg ttgcctgcgc cttccatcga caccggtgct
     2401 ggcctcgagc gcatcgcggt cgtgaagcag ggcgtcctga gcaactacga caccgacctc
     2461 ttgcgtccaa tcgtcgactt ggctgctgag atttcgggga agcgctacgg ggagagccaa
     2521 tcccatgacg acgtcagcat gagagtcatt gcggaccatg cccgaacgac ggcgtttctc
     2581 atttcggagg gcgtcttccc agacaaagat ggccgcgcct acgtccttcg ccgcgtgatg
     2641 cgccgcgcga ttcgccatgg ccatcggctc ggaatcggcg aacctttttt tcaccactgc
     2701 gcgcttcgtg tggttgacga catgggggac gtgtatcccc agctcgtcga acggcgtgcg
     2761 ctcatcgagg agatcaccca acaagaagag gagcgattcc ggcggactct cgaccgggga
     2821 ctccaactga tttccgaaaa tcacgaatgg atcgaaagca acggtgcgcg ctccatgccc
     2881 ggagccgtag cgttcaagct ctatgacact tacggattcc ccctcgacct tcagaacgta
     2941 atcggcgagg agcagggctt cacgatcgat caagatggct tcgatagaga gcttggcgag
     3001 gctcgtttgc gcagccaggg ctcgaaggtc ggcagcagtg cggtcgccga gatctacccg
     3061 gcgctggtgc aagaggtggg ggcggtcgag ttcctcggat acgacagcga ggttgcgcgg
     3121 agcgaggtcc tcgccttggt gcagagcggt gactccgtaa aggagctcgg ctccggtgca
     3181 tcgggtgaga tcgtcacacg cgctacgccg ttttatccgg agcaaggcgg ccaagtcggt
     3241 gaccggggtg tgatcgagct cgcgggcgcg cgcttcgaag ttgaggatac ccaaaagccc
     3301 gtcgaaggat tgatcgtcca tcggggcaag gtcatctcgg gttcgttcca gccgggcgct
     3361 gaggtcgagc tccgagtcga tcatgggctt cgaagcgcca cgcggcggaa ccatagcgcg
     3421 acccatctct tgcactgggc tttgcgtcag gtcgttggcg ctaccgctgc tcagaagggc
     3481 tcgctcgtag gtcctgaacg tcttcgcttc gactatagcg gcacccgccc actcgaagcc
     3541 gccgagatcg agcgaatcga agacctggtg aacgcgcgag tgctcgagaa caccgatatc
     3601 accaccgaag cgctggcgat ggacgcggcc aaggagcgag gcgcgatcgg catctttgaa
     3661 gagaagtacg gggatgtggt gcgtatgctc acgatcggcc cgtcgctgga gttgtgtggc
     3721 ggaacccacg cggcgcgcac gggcgacatc gggttgttca aggttctctc agagacgggt
     3781 ctggcagcag gagtgcgccg catcgaggcc accacggggc tgaacgcgct ccgccatctg
     3841 cgtcggctgg attccgagct cagcggcgcg gccgcattgc tcaaggcccc ggctcagcag
     3901 cttcgcgatc gggtcgaacg tctgatcgag cagcagaagg agtcgcagcg cgagatcgat
     3961 cgcctcaagc gctcactgat gtcgggcgac agcgcggacc tctctgccga tgcgcgcgaa
     4021 gtggacggcg cgcgggttct cggtgccgta gtggagttgg gcgatgccgg cgcgctgcgt
     4081 gaaatggccg accaattgcg agacaagctc gcgccagcgg tgatcttgct gggctcaccg
     4141 agcaaagacg ggaagaaagc cctgctcgtc tgcagtgttt cgaagggact gaccaaacga
     4201 ttccgggcgg gcgacatcgt caaggccgcc gcttcgatcg tcggaggcaa cggtggcggg
     4261 cggccggact tcgcgcaggc cggcggaccc gacgcgacca agatcgaaga cgcggtgcgc
     4321 caggtctatg agctcactgg agtcgcctag gtgtcggcga agtcgaaact cgttggcgcg
     4381 tttctcaacg tctatttgaa ttcccgtccg gctaagcacg cgcttcatcg cgccggcagg
     4441 gtcttacgcg gtggtcgccg ccgcgcacgc ctctactacc gtgccgacga tccctacagt
     4501 cacttgctgg ctcaggtcgc gccgcgattg cgggatgcat acgggctcga gatccagatc
     4561 attcccgtcg ctcatccgag catggccgcc aatccagccc ccgaaatgct tctggaacac
     4621 gcgatccagg acgctgcgat actcgccgag agctacgggc tcagcttccc cctggcggct
     4681 caggctcccg ctgaagatcg cgtccggcgt gctcacgccg tccttctcga acgacggccc
     4741 gccgacgaac aagtcgacgt ggcgatcgag ctcggcgaag cggtctggcg caatgatggc
     4801 acctcgctgg cggcgatcgt cgatcgccat ggcagtctct ccggcgaatc cgtccgtcca
     4861 gcgctcgagg ccaactacac cgcgctcgag cggaccggtc actatcagcc cgggatgctc
     4921 tactacgacg gcgactggta ctgggggatc gaccgacttc actacctcga agaaaggctc
     4981 cagcgcgaac ggctcgaagg gaacatcagt ctcacgcgag gttccacccc ggagctggcg
     5041 aggctgcttc gccgcccaga agggagcagg ccaggcatgg aggctttcct ttcatttcgg
     5101 agcccctatt cgtatcttgc agttccgcag ctgatccagc tccgcgatcg ttacggggtc
     5161 gacgtcgacg tgcgtcccgt cttgccgatg gtgatgcgcg gcttgcccgt ctctctagca
     5221 aagaggctct acatcgttca cgatgccaag cgcgaagccg ataggctcgg aatccctttc
     5281 ggccgggtga gcgacccgct cggagagggc atcggatatt gcatggcggt gttcttcaac
     5341 tgtgccgtcg acaaggggct tggactcgag ttcgcgcgct ccgtgatgca gggcatctgg
     5401 gcagaggctc gagacgtcgc ccacgttccc gacctcctgt tcctggccga gcgcgcaggg
     5461 atcgccgagg ccgaggtgat ggccgctctg gagacgggca actgggagca gagagcccag
     5521 gcgaaccgcg aggccctcac cgaaatgggg ctctggggcg taccctcgtt tcgaatcggg
     5581 gagtattcca cctggggtca ggaccggatt tcgttcatcg agtcggagat cgctcggttt
     5641 tctgcccccg cctaggtgtc tttttgaagc gaaagacttt acggagcctg cttttcctgt
     5701 gacattgatt acgtgaccgt ttccgaagag aagctggctg gggcggacca ggcactcgcg
     5761 gagctcggaa ggagcgaagc cgagatcgcc gaggtacgag gtcgttttgc gaatgcggcg
     5821 gccgtggatc tcgaggccac cgatgcaatc ttgagcacgc ttgccgaagg tgtctcggtc
     5881 gagctcacgt caagcgaggc gcgagcctcg gtcgctcaat cggtcgaaga cgtttgggac
     5941 gacgagcaca ccgaggtcga gatcatcgac gaatccgatt tcgtgctcct cgtggacgag
     6001 aacgagctcg aagagctcga gaaagtcggc gaagacgacg ccgtgcggtc gctccctcct
     6061 gcgcttccgc aggaaggcgc tgacggcgaa gaaggcgatg gcttcttcaa gaagcttttc
     6121 ggcaaccgtc ggagcagcaa ccggccatag cggcggggct ccgattcaga aatagaaagt
     6181 cgcaccaaag gcaagcaccg cgccacccga cgtgtcggtg cttcgcgtgc catcgaagaa
     6241 ttcgggtcga ggatcgttgt cgattcggtg ccggagcacg cctcgcacgt cgacgttcaa
     6301 cgcaaacgcc ggcgcgagcc tcagctcgag gcccatgccc gcctgccctc cgatgtacgt
     6361 catgtgacgg agctcgtcgt acgtgtcgac acgcccgaac gagacgcctg ggcccagaag
     6421 aaagtacact tgcgccttgt gctgcgggtt gacgaagaac agcacattga cggtcagtgg
     6481 gatctccgtt cggtagaagc cctggtagtc gttgcccgcg aagtagcccg aaccgaggtc
     6541 gatggccacg tgctccttgg gccgaaagcg cagcgcaccg gagagcccac ccatcaggac
     6601 ctgcggccca aaagtaccgc cgacgtcgaa gtgcagaccc agttttcggt cgcgcttggg
     6661 cggtgtcggg gcttgggctt gcggctgtgg gattgactgt cgctcgacca cgacgacggt
     6721 cggcggtttc gtcttgacga tcaccgggga cgactggtcg ttgggttgga ccacgacgac
     6781 ctcggcccgt gccgcctcgg tgctaccgaa gacgagggcg aaggcgaggg aagggagcag
     6841 aacgaatcgg atcattgggc acctctcggc tttttacgag gggacccggt caaagattca
     6901 accgaggcga gtcaatgcag gaaacgccct gatttcaagc ttctaatttg ctcatcgaac
     6961 ggcgcgacgg tcttcgaggg cgcggcccag cgtcacctgg tcggtaaact ccagctcgcc
     7021 accatgcggc actccggctg caatccgtga gacgcgtacc gcagaagcgg agagcgcatc
     7081 cgcgacgtag agcgcagttg cttccccctc tacattgagt ggcgttgcta cgatgacctc
     7141 attcacgcct tcctctgcga tgcgccgttc gagtgcgtcg agtggcagct gattcggtcc
     7201 gattccatcg agcggcgcca gcaaaccatg caagacgtgg tagcgacctc gaaagctacc
     7261 gagcgcttcg agcgcgatca aatcagggac tttcgcaacg acgcagatta gggaggcgtc
     7321 acggcgcggg tcccggcaaa tcgcacagcg cgactccgca ccatagtttc cgcagagctc
     7381 gcatcgggtg acttgatcat ggatgccacg cagggcctcg cccagcgcct ctgcatattg
     7441 ctgctccccc tctagtacgt gaaaggcaag ccgcgccgcg gttcgcgtgc cgatccctgg
     7501 caagcgggag agcaacgaaa tcaaagacgc aatcggatcg ttggcggtca tcggtcagcc
     7561 gattccgggg atcgaaaggc ctcccgtgat cttttcgatc tccgcgtcga cgtgctcgcg
     7621 tgcctttgtc agggcagcgt tggctgcggc gacgacgagg tcttgcacca tttcgatgtc
     7681 ctcttcttcg aggagggtct tatcgatgcg gatttcggcc agctccggaa tgccgttcgc
     7741 gatcgccgtc accttgtcgt tgccagcgcg agcttcgaac gtttcctctc gcagctcttc
     7801 tctgcgcttc tcgaccttgc gctggacgcg acccgcttgc cgcatcagct ctcccattcc
     7861 gccacgaaaa ttcattcgat cctctcagtc cgcttcgatg agcactttca gcttcccggc
     7921 cgattcagga aacacgtcga cggcatcacg cacgcgtggg tggttgagcg cttctttgcg
     7981 cttggcgtcg agctcggcct cccttcgctg ggctgcgact gccgcgaccg tcttcgtcgc
     8041 tgcctcgccc tgagcatcga agcggatctc gatttccgga cgtccgccga gctgctgttc
     8101 ggctatccgc acgattgcag cgatcgattc gggcgatccc gcttggcgac cgtagaacga
     8161 gccgtcctta aacgaaagga cgatccggtc ggggccgacg gtcttgggaa tgccgtgttc
     8221 gaggacggcc gccaaagccg gttgctgttg ctcgagggtg ccgaccagct cttcccattc
     8281 ctggagggcg gctcttcgga tcggcgtcag tacctcgccg gtgctcgtct gaaagccacc
     8341 ggcgtgtttc gtcgccggca tgaccggctg attgggattc tggggtttgg gttctgggtc
     8401 cgacgcagag cgtggtgcgg cgtcgacttc gaccttgggc ttgggtgcag gtgtcgcttc
     8461 cgctttcggc ttggctggag ccggttccgg ctttggcttc gtcgtgcgag cacgcgaacc
     8521 accgggtgag ccgccactcg gccctggctt gccctgtaac ccatccagtc gcgcaatcag
     8581 ctcagcgacc gactgaagtg gaggcctggt cgcgacccgc acgagcccca tttcgagcat
     8641 gagctttggg ttggggcttt gggccatttc gtccatgacg atagacgcag ctcgaaacgc
     8701 gcgctgaagc tcgagaatgt cgacgtcttc gatcagctgc atggcggttt cccgctcttc
     8761 ggggacccag tcggcggggt ccccagcgtc gagcttgagc accacgaggt cgcggatgag
     8821 ctgcagcagc tggcgcgcaa agtgttgcat gtcgacgctc ttgtcggcga ggccatcgac
     8881 agctttcacc acgcgagcgc cgtcgccttc gagcacggcg ctcgcaacct cgtacaaggc
     8941 ctcgcgcgcg gcgatgccca ggcttgcagc cacggtatcc gcggtgattc gagcgtccga
     9001 gatcgcgacg agttgatcga gcagggtgag cgcgtcgcgc atggaccctg ccgcctccct
     9061 cgcgaccacc gagatcgctt cgtcgtcagc cgagagggat tccgtctcga gaatctcacg
     9121 aacgcgctcg gcgatgagcc ggtgcggaat cagtcgaaag tcgtactgct gacaccgtga
     9181 gcgaatggtg atcggaacct tgtggctttc cgtggtggca aagataaatt tcacgtggtc
     9241 cggcggctct tcgagagtct tcaaaaacgc gttgaaggcg cccgccgaga gcatgtgaac
     9301 ctcgtctacg atcacgatct tgtagcggtc ccgctggggt cgataaggga gcgactcttg
     9361 aaggcgccgc acgtcttcga cgctgttgtt cgaggctcca tcgatctcct ggacgtcgac
     9421 gtcgataccg gccgtgatgt cgcggcaagg gccacagtcg ttgcacgggg tcggagtcgg
     9481 cccgttctcg cagttcaagg cctttgcaag caggcgcgcg gtggtcgtct taccgacgcc
     9541 tcggacaccg gtgaacagga atgcatgggc aaccctgtcc gactcgatcg cgtttgcgag
     9601 agtccgcgcg acgtgctcct gacctacgag atcttcgaag ctttgcggtc ggtatttcct
     9661 cgcgagaacg gtgtatgcca tcgggcgagc gagcctatca cggcagcccg ctcggcgcag
     9721 ccgcactcgc tcggccgact cagagctcgc agcggtctgg ggccaggtgc ctattcgacg
     9781 tcgtcatcgc ccaagtcctc gtcatcgccg aggtcctcgt catcgtcgaa gtcttcgtcg
     9841 tcgtcgaggt cctcgtcgtc gtccccgtca tcatcggctt ccatggcttc ttgcatcatc
     9901 tcttccatgg tgggcatgcc gcctgcatcc tcgccccgat cgtctacgcc gtcgccgtcc
     9961 tcgtcgagca gtgcattggg atcagcaccc atgatcgcgt tgccgagtcc gatcgacacc
    10021 tgcggatacc aggtaatgat gccgagaccg atcagcatga ggccgacgaa gggcgcgacg
    10081 ctccgtatga tgtggccgag ccccttttca tagagtgcgc tggctacgaa gaggttcagg
    10141 ccgaccggtg gggtgaggta gccgatctcg aggttgacga tgaagatgat cccgaaatgc
    10201 atcggatcga cgcccatcga ggccgcgatc ggcgccaaca agggcacgaa gacgaacatc
    10261 gcactgagga tgtccatcat catcccgacg gccaggagca gcaggttcag caggactagg
    10321 aactgccagt actcgaggtc cttgccctga atccactcga ccattcgagc gggaatctcc
    10381 tctttctcga ggaactcacc gagcgcgagc gccatgacca tgatcaccag gaaggaaccg
    10441 aggaacacgc ccgtttcgcc gaagatcgcg gggatggctt tgagcttgag cgcgcggtgc
    10501 agccagacct cgaccacgat cgcgtacatg acgctgacgg ccgcggcttc gaccgcgttg
    10561 aagataccgc cgtaaatgcc gccgaggatc agcaggggga acatcagtgc ccagaagccg
    10621 tcgcgactgg ccgcgatgag cttctggaac ttgaacggtg aggtcggaat cttgtcgacg
    10681 atgccctgat agaacgagta accgatcagg atcgagccca tcacgaggcc ggggccgaag
    10741 ccggaggcga acaggcgctc gacctcgatg accgcgccct gattgacgat cgggtagacg
    10801 atcatcggaa tcgacggggg gatgagaatg ccgagggaac ccgcggaggt gagcaggccg
    10861 tgggagaagc gctccctgta gccctcggcg atgagcgcag gtcccatcat ggagccgatc
    10921 gcgaccaccg ttgcggggct cgagccgctg atcgcggcga agagcatgca tgcgagaaca
    10981 ccgctcatcg ccatgccgcc ggggacccaa ccgatgaacg cctgggcgaa ttcgatcaag
    11041 cgctggctga tctgtccctt gctcatgacc gcgccgctca tgatgaagaa cggaatggcg
    11101 agaagcgtgg ggctgtcggc gaggctccga atgccttcga tcacgatcgt gaactggttg
    11161 agctcggtga atccgctcca aaggaacagg ctgccgagcg tagcgacgcc gatcagcacg
    11221 aacagagggg agccgacgag cgcagcggcg atgatggcgg acgcgaggat ccctcccttg
    11281 ccgaggaacg gcaggaagac gatgatcgtg accatgatga ggaagggcag cttccgttcg
    11341 cgtacggcgt tcatccttcc gctccctgct ttgcggcctt tgcttgctca gcaagagcca
    11401 gggcctcatc ttcaggggcg gcgccgtacg agccccccat gatggagctg aacgccgcgg
    11461 ccacgtagcg aatcgtcgtc atggcgaacg cggccggaac cgcgatggtc gcgacccagt
    11521 cggggatgtt ggtttgctcg aatcgaccct cgagctggag ggcttccttg gcgtacacgt
    11581 agccgagcgc agccatgaag aagcagaagc cggctgtcgt gatgtagccg attgcatcgg
    11641 tccatttcga caaggatggg gggacgactc gtttcagcgc gccgacttga atgtgcttgc
    11701 cctcgtgtgc gcatacgctc gcgccgagga agccgaccca gagcagcatg atcagtgaga
    11761 gctctttcga ccagctgtag ccgtccggga agtagttgac gatgatgtag accaggagcg
    11821 cggtgacgac gaagaaggct atcagctttg agacccaacc cgctgcacgc tggcgcagca
    11881 actggacgag ccatgccaat gcacagagcc cgtacaggag tagatagaac cagcgagact
    11941 cgaccgtcgg ctggagcatg atccagccca agagcccgag accactgcag gccacgatgg
    12001 cctcgactat cggtttggtc cgcgaacgct ttccgggctc ggccgcgtgg ttctccgcag
    12061 tccaaaacgc gaaatacacg aggccaatgc ccacgaccgc cgtggcggta ggaccgatcg
    12121 tggtcagtgt cttcattcct tcgggagtct cgacgccgaa gatgcgagcg cagagctcgg
    12181 ccaccttgct atccggagcc gcgagccgcc ggtaaacgac atccaagaac accatgatcg
    12241 cggacgcgcc gaggaaggtc caaagcacgc ccatttcgac gtggaagatc caatcgtcga
    12301 tccgccgaat gatgccaaac cgaccctcgt aggggttctc tttgcccggt tccgccatag
    12361 cgccggacgg tacgtttgat cgtgctggca atcaagactt gttgctgtag ggtcgtcaaa
    12421 gaagacatgc gtacagtcat ccacttcgac atggacgcgt tttttgcgtc ggtggagcaa
    12481 ttggacgacc cggatttgcg cggaaagccg gttctggttg gcgcccgctc gcgcagaggc
    12541 gtggtcgccg ctgcctccta tgaggcacgt ccaagcggag tgcgctcggc gatgtcgatg
    12601 gtcgaggccc tccgtcgatg ccccgacgcg atcgtggttc cgccacgaag gagtcgctat
    12661 tcggagctga gtaccaaggt ttttcaggtt ttccggcgct acacaccgct cgtggaaggc
    12721 ctgtcgctgg acgaggcctt tctcgacgtc accgccagcc ggtctctttt cggtgatggg
    12781 cctgaaatcg cgcgccgcat caagaaggag gtgcgcgccg agaccgggct cagcgggtcg
    12841 gcgggggtcg cggccaacaa gttcgtcgcc aagatcgcca gcgacctcca taagccagat
    12901 gggttgacca tcgtccccgc tgacgcctcc gcggagtttc tcgatcccct cccactggag
    12961 cggatgtggc gggtcggtcc gaaggcaagg gtccggctgc gggacgccgg tctacaaacg
    13021 atcggcgacc tcgcgcgcac cgacgttcgt acactcgagc gcttgctcgg gacttggggg
    13081 cccgttgcga gggatttagc acgcggactc gatgagcgac cggtcgtcgt cggcgcgccg
    13141 cccaagagcc ttgggagcga atcgaccttc gagcaagacc tcagaagctg cgaggcgctt
    13201 ctcaagccga tcctcgagca atcgatgttg ctggccgatc gactcgtgcg caaggggctc
    13261 tgggcaaatg tggtcaccct caagatcaag tacggcgacc atcgcattcg cacgcgccag
    13321 attcggctag cgcgggcggt ctccgacacc gacgcgatct tcgaagcggc cactgagctg
    13381 ctcacgcgct tcgatgctct tggtgccggt gttcgcctga ccggggtctc ggtttcgtcg
    13441 ctcactaagg ttcccgatgc ggagctgttt ccggacgccg agcgagcgcg gcgcgagcgt
    13501 ctcgcggcga cgacaggagc gcttcgcgag cgcttcggaa ccgccagtct cacacgtgct
    13561 tcgttgctcg cgagcccgga cgatatcggt gattcgcacc gctagaaaat tcgcagaatc
    13621 acggtatcgg caacatcaac caagagtcgc gttgacacca tcggggacgg catctagggt
    13681 ccggctgttg cgtgcaacac gcaatgctct aggagcccca ccggatgaac gcacgacaca
    13741 ccaagttcat cttcgtcaca gggggtgttg tcagctcaat cggaaaaggc ctagcggctg
    13801 cttcgatggg cgccctcctc gaagcacgag gattgcgggt caccaacatg aagttggacc
    13861 cgtacatcaa cgtcgacccc ggcacgatgt cgcctttcca acatggagag gtcttcgtga
    13921 ccgacgatgg agcggaagcc gacctcgacc tcggtcacta cgagcgcttc accaaagcga
    13981 cgatgaccaa ggcgaacaat ttcacgaccg gcaagatcta cgattccgtg atctcgaagg
    14041 agcgccgcgg ggagtatctc ggcgcgacga ttcaggtgat tccgcacatc accgacgaga
    14101 tcaaggccag agttcgagag gcgacccgca acgtagacgt tgcgatcgtc gaagtgggcg
    14161 ggactgtcgg tgacatcgag tcgcttccgt ttctcgaagc ggtccggcaa cttcgcggag
    14221 agctcggccc gcagaatagc gtttcagttc acgtcacgct cattccgcac atcgcagcgg
    14281 ccggcgagct caaaaccaag cccacccagc actcggtaaa ggagcttttg ggactgggaa
    14341 ttcagcccga gattcttctt tgtcgcgtcg atcgcgagct acctcatgcg ctcaaggaaa
    14401 agatcgccca cttctgcaac gtgcccgtca aagcggtcat cgccgcccca gacgttcgcg
    14461 tgatttacga gcttccgctg acgctccacg agcagggtct cgatcgcctc ttggccgagc
    14521 ggctcaacat ctggtcgcgc gatccggatc tctcggtttg gcggcgtacg gtcgagcgtt
    14581 atctggctcc ggaccgcgga cacgtttcaa ttgcgctcgt cggcaagtac gtccacctcc
    14641 gcgattcgta caagtcgctc cacgagtcct tggttcacgc ggcgctcgag tgtgatgtga
    14701 agctcgacct ggactacatc gacagcgaag agctcgaaag cggcaacgtc gaggcgcgac
    14761 tcagccggca cgacgcgatc ctcgtccccg gtggtttcgg agaccgtggt gcggaaggta
    14821 agattctggc aattcagttt gcgcgggaga acgacattcc cttcttcggt atctgtctcg
    14881 gcatgcagat ggcggtcgtg gagtacgcgc gaaacgtttg cggcctgcaa ggcgccaaca
    14941 gcgccgagtt tgatcctcag gcggagcacc ttgtggtcga catcatgccc gaccagcgcg
    15001 gcgtcgaaga caagggcgcg acgatgcgcc tcggcgccta cccctgcacc atcaaaccag
    15061 actctctggc tctgcagatc tacggtacgt cccgcatcag cgagcggcac cggcaccgct
    15121 tcgaggtcaa caacgcgtac cgcgagcggc tcgaggatgg tggcctggtg ctctccgggg
    15181 tctcgcctga tgggcgcctg gtcgagatgg tggagctgcc ggcacacgcc catttcatcg
    15241 gctgccagtt ccaccccgag ttcaagagca aaccgcacac cccgcatcct ctgttcacga
    15301 gcttcgtccg agcggcgatc gagcaccacg cccgtcgagg aaagtcggtg agcggcgcaa
    15361 ccgaagcact cgtcaactga gcgctgctca agtttcgcgc cgcgtgggag aattggtctc
    15421 cggcggcggc ccgggagggg ctcgctgatt gaatccgaga ttcccgtggg gtattccggc
    15481 gctcatgtac ggggagcaac caccgcgctc agatctcgag tcggtatggg atggctaccg
    15541 acaggctctc cagcgcgggt cgagtccatt cggcgcggcg ctcgaggtcg cgtcgagtgt
    15601 cgaccggctg ggcttggcgt tcgcagccgg ctattccgca gcgcttcaaa ccctggtgcc
    15661 ggacgtcgtc ttgccctgcg cgctttgcgt gaccgaaatc gagggcagcc acccacgcgc
    15721 gatcaaaacg actcttcgcg ccgccgaggt cggctcctcg tatgcgctca acggcacgaa
    15781 gacattcgtc accttcggga acctggcaaa gacgttgatc atcgccgcgc gcgcaggcga
    15841 gcatgcggat gggacgcccg agctcgttgt ggtgcgcatt ccgaccgacc gagacggcgt
    15901 ctcattgatc gatctgccac cgatttcgtt cgttcctgag gttccgcatt cgcgcgtcga
    15961 gctcgagaat gtgtgcgtcg accccagcga gcgcatgagc ggcgacgggt atctcgagta
    16021 tgtgaagccg tttcgaaccg tcgaggacat tcatgtctac ggcagcgctg tcgcgtactt
    16081 tttcggcttg ggcaaacgga ccggtgcgcc agccggcttc ctggcggaga tggtatcgat
    16141 cctcgtggca ctcgagagcc tgcgcgaggc gacaccattg gatccaaaca cgcacgtggc
    16201 ccttcatgga gtgggcgagc atttgtcgca cctcattacc agtgatgagt tcctgggcat
    16261 atgggacgcg gctccgcctg aggagcgaga tcgctgggag cgcgatcgaa aactcctcga
    16321 cgtggccgga agtgcacgcc gcgcgcgctt tcagaaggcc gccacgcaac ttgggctgtg
    16381 ttagcctttc gctgctcgtt ggacgcggcg gatccagtgg ctccgctcag cggatcgctt
    16441 gcgaatcagg ggcggttttc gcagtctggc ggtcgcgagg actagaacct cgccaggttc
    16501 cgctaccagg tctaggatcc acccatgact cgtccggctc gccggaaatg gctcacgatc
    16561 ccgactcgca tcgtgcttgg ctacatactg gtgctcgtcg ccttcgccgt cgtaggtagc
    16621 ttgagcttcg tccagcacca tcggacgtcg cagacgctcg ggctgctcga ggacggatac
    16681 cttcctttgg cgctcacgtt gagtcaggcc aaggcgacgc agagc
//
LOCUS       DASRZF010000324         3736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3687128, whole genome shotgun sequence.
ACCESSION   DASRZF010000324 DASRZF010000000
VERSION     DASRZF010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3687128"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VFG22_14235"
     CDS             complement(<1..347)
                     /locus_tag="VFG22_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417452.1"
                     /translation="MTRTFACWGAAMLVAGCWNTEAPSPDAQHDSANETRSESPQMPI
                     ETPTLERRDQEWSGAASLAVVEDPLAASAADAPATADSPATAANAQPDESTQRDLAAE
                     LKAALGIPGDCLQ"
     gene            complement(455..1468)
                     /locus_tag="VFG22_14240"
     CDS             complement(455..1468)
                     /locus_tag="VFG22_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417453.1"
                     /translation="MFQKLMLVMFLLATAACQPKFTPQSTQVPEYRRPQRVLPTIRIL
                     DAGLTPRAPLRYRVPPGASETVYLELVRAQALRVGNEGGQSGMPPIQLQVQMGPSEPT
                     PEGFIRHPLKITQLRIANEAQKMSPAQREQMEKTLAPLLHVEGWSEMDIQGRVRRSEF
                     RGLEDVPPDLIAMLGNIRTALLTVPFPDEPLGVRSRWEVERKIQLSGFWADQVATYHI
                     VKMDRNQIELQIAARQSATPQSLANGRLEAYQVSVIGSAVVRLDSLSAFSEAEATSQM
                     RIQQRTGSGSQLLQVDSRTMVRLYPADAGKPLAEEAPEQDAVDQEDANVITDPGKQKL
                     KWR"
     gene            complement(1474..2397)
                     /locus_tag="VFG22_14245"
     CDS             complement(1474..2397)
                     /locus_tag="VFG22_14245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417454.1"
                     /translation="MNRLIIAASFVAALVTLGCKKEKTAPPPPELAVTMIDSGDAPRE
                     PLRYAIAPGTVVQSILEIRSVDEAHERTEKSQNAFGVLPGFRLLLRAGPTVLQAKGAR
                     YVLRISKADSILPEGVTERQAEEVERGIQVLRGMRGRFDINEQGIVIDADVPWAQGHQ
                     ALHPRIAITIGNVRSAIATIPVPNEPVGVGAIWEVRRPLRIWSARVTQVTRYQLTDRA
                     GDRFRVAVTVDQVAAPQVADLNPDLEMRVRSYEMHARGHVLADLGLPLALEASLESHS
                     AADIALVSPEATEHLTSSRRSVLRLVSKSER"
     gene            2466..3338
                     /locus_tag="VFG22_14250"
     CDS             2466..3338
                     /locus_tag="VFG22_14250"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HET6417455.1"
                     /translation="MSTSRWTKGCDATRHLSVLDDSVKQLIDRPQTLILWLTGAGVSA
                     ESGIPTFRGEEGYWRVGSANYQPEQMATRAAFRQMPNEVWAWYLYRRGVCRSAEPNAA
                     HHALVKAEGRGAKRFLLVTQNVDGLHLRAGSTPERTYQIHGNIDFMRCAKECLPAPVP
                     IPDGIDLAWSKGRSISEPELQQLRCPACGAMARPHVLWFDETYDEPNFRFQSSIDAAR
                     RASLVVVVGTSGATSLPMHIGTIAAQRGVPMIVVNPEPNPFSAFVRRTGRGAFLAGTA
                     GEVVPELVRALGSA"
     gene            3381..>3736
                     /locus_tag="VFG22_14255"
     CDS             3381..>3736
                     /locus_tag="VFG22_14255"
                     /inference="COORDINATES: protein motif:HMM:NF012280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417456.1"
                     /translation="MARLFVWIFCIGFLSGCAKDGEEARYPDRPGGACSRAAGASCDA
                     EFVCHYAQEAECGETGAQGACFPMPSTCTQDYSPVCGCDGRTYLNICVAHSHGVSPRL
                     PGTCPNAALPAGPAGS"
BASE COUNT          650 a         1170 c         1201 g          715 t
ORIGIN      
        1 tcctgcaggc agtcgccggg aattcccaga gcagccttga gctccgcggc caagtcgcgc
       61 tgggtgcttt cgtctggctg agcgttcgcc gcggtcgcag ggctgtccgc ggtcgcagga
      121 gcgtccgcgg cggacgcggc aagcgggtct tccacgacgg cgaggctcgc ggcgccagac
      181 cactcttgat cgcgccgctc gagcgttggc gtctcgatag gcatctgcgg actttcgctg
      241 cgcgtctcgt tcgccgaatc gtgctgcgcg tccgggctcg gtgcctccgt gttccaacag
      301 cccgcgacga gcatcgcggc gccccagcag gcaaaagtgc gcgtcatggc tcgatcctag
      361 cccaaaatgc gaagcggcgg aatgggcgag caaagcccag gactcgtgaa tctccgtcga
      421 ggtccatccg acgactcgac gctggatcgg cggctcatcg ccacttgagc ttctgcttac
      481 ccggatccgt gatcacattg gcgtcttcct ggtcgaccgc gtcttgctcc ggtgcttcct
      541 ctgcgagcgg ttttccggcg tctgccggat agaggcggac catggttcgg gagtcgacct
      601 gcagcagttg cgatccagag ccggtccgct gctgaatgcg catctgcgaa gtggcttccg
      661 cctcggaaaa ggcgctgaga gaatccaatc gcacgacggc cgagccgatc accgagactt
      721 gatacgcctc tagtcgaccg ttcgccagcg attgtggagt tgcagactgc cgcgccgcga
      781 tctgaagctc gatctgattt cggtccatct tgacgatgtg ataggtcgcc acctgatcgg
      841 cccaaaaccc ggagagctgt atcttgcgct cgacttccca gcgtgaacgg actccgagcg
      901 gctcgtcggg gaacggcact gtgagcaagg ccgtgcgaat attgcccagc atcgcgatca
      961 gatcgggtgg cacgtcttca agtcctcgga attcgctccg gcgtacgcgg ccctgaatgt
     1021 ccatctcact ccagccctcg acatgcaaaa gcggcgcaag cgtcttctcc atctgctcgc
     1081 gttgggccgg gctcatcttc tgagcctcgt tcgcgatgcg cagctgagtg atcttcagcg
     1141 gatggcgaat gaaaccctcg ggagttggct ccgaaggtcc catctggact tggagttgaa
     1201 taggcggcat cccactttgc cccccttcgt tgccaactcg cagcgcctgg gcgcgaacca
     1261 actcgaggta gacggtttcg ctcgctccgg gtgggaccct atagcgaagt ggagcacgcg
     1321 gtgtgagtcc ggcgtccaga atccgaattg ttggcaggac acgctgcgga cgccggtatt
     1381 cggggacctg agtggactga ggcgtaaatt tgggttggca ggcggctgtc gccagtagga
     1441 acatgacgag catgagtttc tgaaacacgg atctcacctt tcgcttttcg acaccagtcg
     1501 tagcacggac cgtcgcgatg acgtgaggtg ttccgtcgcc tctggggaaa ccaacgcgat
     1561 atcggcggcc gagtgcgact cgagcgatgc ctcgagtgcg agcggaagac ccaggtccgc
     1621 gagtacgtga ccgcgcgcgt gcatctcgta gctacgcacg cgcatctcga gatccggatt
     1681 gaggtccgcg acctgaggcg ccgcgacctg gtccaccgtc acggcaactc ggaatcgatc
     1741 gcctgcgcga tcggtcagct ggtagcgtgt cacctgggtt acgcgggcgc tccagatgcg
     1801 caaggggcgc cgtacttccc agatcgcacc gacgcctacg ggctcgttcg ggacaggaat
     1861 ggtcgctatg gcggaacgca cattgccaat ggtgatcgca attcgagggt gaagtgcttg
     1921 gtggccctga gcccagggaa cgtccgcgtc gatgacgatg ccctgctcat tgatgtcgaa
     1981 tcggccgcgc ataccgcgaa ggacttgaat gccccgctcc acctcctccg cttgacgctc
     2041 cgttacgccc tctggaagaa tcgagtctgc ctttgagatc cgcaagacgt accgtgcgcc
     2101 cttcgcctga aggaccgtcg gcccggcgcg gagcaatagc ctgaacccag gcagcacacc
     2161 aaacgcgttc tgtgatttct ccgtccgctc gtgcgcctca tcgacacttc gaatctcgag
     2221 gatcgactgg acgacggtgc cgggcgctat cgcgtagcgc aagggctcgc gaggggcgtc
     2281 cccgctgtcg atcatggtga cggcgagctc cgggggcggc ggtgccgtct tctctttctt
     2341 gcagcccagc gtgacgagcg cagcaacgaa gcttgctgcg atgatgagtc tattcatttg
     2401 ccgccgaagt tcctttgctg ccgtgtcttc tagagagtaa tgaacttcga ggcaagcgtg
     2461 ggtcgatgtc gacttcgcgg tggacgaagg gttgcgatgc cactaggcac ctcagcgtgc
     2521 tcgatgactc cgtgaagcaa ctcatcgacc ggccgcagac gctgatcctc tggctcaccg
     2581 gcgccggggt ctccgccgag agcggcatcc ccacctttcg aggcgaggaa ggctattggc
     2641 gggtcggttc ggcgaactat cagccagagc aaatggcgac cagggccgcg tttcggcaaa
     2701 tgccaaacga ggtctgggcc tggtacctct atcgccgcgg ggtctgccgg agcgcggaac
     2761 cgaacgcagc gcaccatgcg ctcgtcaaag cggagggacg cggggccaag cgattcctcc
     2821 tggtcactca gaacgtggac ggacttcacc tgcgcgctgg cagcacgccc gagcgcacct
     2881 accagatcca cgggaacatc gatttcatgc gctgcgcgaa ggaatgcctc cctgccccgg
     2941 tgccgattcc cgacggcatc gatctggcct ggtccaaggg gcgctctatc agcgagcctg
     3001 agctccagca gttgcgctgc cctgcctgtg gcgcgatggc acgaccgcac gtcctttggt
     3061 tcgacgagac ctacgacgaa ccgaactttc gtttccagag ctcgatcgat gcggcgcggc
     3121 gcgcctccct cgtggtcgtc gttggcacta gcggagcgac ttcgctaccg atgcacatcg
     3181 gcaccattgc ggcgcagcgg ggagttccga tgattgtcgt caacccggag ccgaatccct
     3241 tcagcgcatt cgtacgacga acgggtcgcg gcgcatttct ggcgggcacg gcgggtgagg
     3301 tcgtgccgga gctcgtacgg gccctcggct cggcgtagcg ggccttgcct tgctcgcggt
     3361 tcggggatac ctacgaagac atggcacgcc ttttcgtatg gatcttctgt atcggtttcc
     3421 tgagcggctg cgcaaaggac ggcgaagaag cgcgttaccc cgatcgacca gggggagcct
     3481 gcagccgagc ggcgggggca agttgcgatg cggagttcgt ctgtcattac gcgcaagagg
     3541 cggaatgcgg tgaaacgggc gctcaagggg cctgctttcc gatgccgtcc acctgcacgc
     3601 aagactacag cccggtctgc ggctgtgatg ggcgcacgta tctcaacatc tgcgtggccc
     3661 actcgcatgg cgtttcaccg agactgcccg ggacctgccc aaatgcagcg ctgccagcgg
     3721 ggccggccgg aagctg
//
LOCUS       DASRZF010000325         3775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4719513, whole genome shotgun sequence.
ACCESSION   DASRZF010000325 DASRZF010000000
VERSION     DASRZF010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4719513"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="VFG22_14260"
     CDS             <1..629
                     /locus_tag="VFG22_14260"
                     /inference="COORDINATES: protein motif:HMM:NF024981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HET6417457.1"
                     /translation="CPGGTASNVIAYLAKADVALSVSMTAFSTMLAAAFTPLLTAWIV
                     GSRVDVDGWGLFLSTAKVVLLPIAVGLFMRRYLPRLTKTLLPAAPAAAVLMIVLIVAA
                     ILAAQKDAVLESGLQLLGAVVTAHSLGFLFGYLLGGLSGGFGRVARTISIEVGMQNSG
                     LGAVLAQAHFANPLTAIPSAISAITHCIIGSALAAVWSRRPALEAPDM"
     gene            678..1949
                     /locus_tag="VFG22_14265"
     CDS             678..1949
                     /locus_tag="VFG22_14265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417458.1"
                     /translation="MLDFKGVPSGAKPMKSMRTTLLIALLLSLPACNVYEADLVEKGL
                     ASIPDRPPPSTSSSSDDVEAIFAFRNISLDQSGDRWRRFGLDLDGMNTKSIGDAAECI
                     AANGNPPLDGDKGIDNAFGQYVLPTVVSLISCLEDNIALNQGSGRGTVLLRLRHWNGT
                     PNDSKVDVAVLSAVDGTSLDDVSGLVWGGAEGSTLMLPETSEGAPDPAWDGEDYFFVD
                     PSSLVAGNLERPEVWKTDAYISQGRLVLPLDTAATFVFLTGPGSFSITLNGFLLADIS
                     EDGQILEKGMIAGRFPAGELVATLKPLGICNDAFRGSVVGLLTDNLDLRVDPDVGSPE
                     DECTATGVAFSFQGIRARIADQIAPVELPIPDPCANVGQEGGPEPAFDRCCRSVELKS
                     PDALPAECSAEDLALYASLPNPIPVPLEEGF"
     gene            complement(1946..2290)
                     /locus_tag="VFG22_14270"
     CDS             complement(1946..2290)
                     /locus_tag="VFG22_14270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HET6417459.1"
                     /translation="MSEAAGITLTPKAVQMAKQKVADAGAPVEGLRLGVKGGGCSGYY
                     YVYDFATKIRPNKDLVFDFDGLKVVIDNRSIEFLKGAVLDWEQSLMSYGFKWKNPNAT
                     GDCGCGESFEVS"
     gene            complement(2341..3546)
                     /locus_tag="VFG22_14275"
     CDS             complement(2341..3546)
                     /locus_tag="VFG22_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET6417460.1"
                     /translation="MSKMPIYMDHQATTPVDERVVEAMLPYFSGRFGNAASRTHAHGR
                     VARDAVERGRSQVADLIGAKPEEVIFTSGATESDNLAVRGVAEARIGDGKHIVTVRTE
                     HKAVLDTCAALEREGWRISRLDVDSNGLLSVDALADALTDETVLVSVMLANNEVGVAQ
                     DLRAIGALTRRRGITFHCDAAQGLGYLPWSLDEMHIDLASLSAHKMYGPKGVGALYVR
                     ESLQTRGIPAPQIHGGGHERGLRSGTLNVPGIVGFGLAAEIMKNEGATEAERIGSLRD
                     ALKNQLFAEIEELRLNGAADPRHPGNLNLSFGYLDGARLLLELAEVVSVSSGSACSSA
                     QPGPSYVLEAMGVPKDWSGASIRFGLGRMSTAPDVQTVGDSVVATVGRLRERSPHWAR
                     RANGKPLDW"
BASE COUNT          648 a         1229 c         1151 g          747 t
ORIGIN      
        1 gctgtcccgg tggaaccgcg tccaacgtga tcgcgtactt ggcgaaagcc gatgttgcgc
       61 tctcggtctc gatgaccgca ttctccacga tgctcgccgc ggcattcaca ccgcttctca
      121 ccgcatggat cgtcggcagc cgcgtggacg tcgacggatg gggcctgttc ctgagcaccg
      181 caaaggtcgt tctacttccg attgcggtgg ggctcttcat gcggcgatat ctgccgaggc
      241 tcaccaagac gcttctgccg gcagcacctg cggccgccgt tctgatgatc gtgctcatcg
      301 tcgcggccat cctcgcagcc caaaaagacg cggtcctcga atccggtctg cagttgctcg
      361 gcgcggtggt gaccgcacac agtctcggat ttctcttcgg ttacctcctg gggggactca
      421 gcggcggctt cggaagggtg gcgcgcacca tctcgatcga ggtcgggatg cagaactcgg
      481 ggctcggagc cgtgctcgca caagcccatt tcgccaatcc gctgaccgcg atccccagcg
      541 ccatttctgc gatcacgcat tgcatcattg ggagcgcgtt ggcggccgtc tggtcccggc
      601 ggccggccct cgaggcgccc gacatgtgag ctaaatcaca ttcgccatga atcttgtgtg
      661 actgttttgt tctagcgatg ctagatttca agggcgtccc gagtggcgcc aaaccgatga
      721 aatcgatgcg gaccaccttg cttatcgctc tcctgctgag cttgccggcg tgcaacgtct
      781 acgaggccga cctcgtagag aaaggactcg cgagcatccc ggaccggccg ccgccgagca
      841 ccagctctag ctctgacgat gtcgaagcga ttttcgcgtt ccgcaacatc tctctcgacc
      901 agtccggcga ccgctggcgc cgctttggac tcgatctcga tggaatgaac acgaagtcga
      961 tcggcgacgc ggcagagtgc atcgcggcca atggcaaccc cccactcgac ggggacaagg
     1021 gaatcgacaa cgccttcggt cagtacgttt tgccaaccgt ggtgagcctg atctcctgcc
     1081 tagaagacaa catcgcgctc aaccaaggat ccggtcgagg cacggtcttg ttgcgcctgc
     1141 ggcactggaa cgggaccccg aacgattcca aagtggatgt ggccgtcctt tccgccgtgg
     1201 atggcacgtc actcgacgac gtttcaggac tggtatgggg cggcgcggag gggtcgacct
     1261 tgatgcttcc tgaaacttct gagggagcgc cagacccggc ctgggacggg gaggactatt
     1321 tcttcgtcga tccgtctagc ctcgttgccg gcaacctcga gcgtccggag gtatggaaga
     1381 ccgacgcata catcagccag ggccgccttg tgcttccctt ggacacggcc gcgacattcg
     1441 tgtttctcac cgggcccggg agcttctcga tcacgctcaa tggtttccta ctcgccgata
     1501 tcagcgagga cgggcaaatc ctcgaaaagg gcatgatcgc ggggcgattt cccgccggtg
     1561 agctcgttgc aactctgaag ccgctcggca tctgcaacga cgcgttccgt ggaagtgtcg
     1621 tcggtctgct gacagacaac ctggatctgc gcgtcgaccc ggacgtggga tccccggagg
     1681 atgaatgcac agcaaccggc gtggctttca gcttccaggg gattagagcc cggatcgccg
     1741 atcagattgc tccagtcgag cttcccattc cggatccttg cgcgaacgtc ggacaagagg
     1801 gcggacccga gccggcgttc gatcgctgct gccgttcggt cgagctcaag agccccgacg
     1861 cgcttcccgc cgagtgcagc gctgaagacc tcgcgctcta cgcgagcctg cccaatccca
     1921 ttccggtgcc gctcgaagaa gggttctaac tgacttcgaa agactcgccg caaccgcagt
     1981 cgccggttgc gttgggattc ttccacttga aaccgtagct catcaagctc tgttcccagt
     2041 cgagcaccgc gccctttagg aactcgatgc tccgattgtc gatcacgact ttgaggccgt
     2101 cgaagtcaaa caccaggtcc ttgttgggac ggatcttcgt cgcgaagtca tagacatagt
     2161 aataacccga gcagccgccg cccttcacac cgagccgaag gccttccacc ggcgcaccag
     2221 cgtcggcgac cttctgcttg gccatttgca cggctttggg ggtcagggtg atacctgctg
     2281 cttcgctcat catcctctat gtaaggcggg acgcgtcgct tcgccagcgt cgctcaatac
     2341 tcaccagtcg agcggtttgc cgttggccct gcgcgcccaa tgtggagagc gctcgcgcag
     2401 ccgcccgacg gtggcgacca cagaatcgcc gacggtctgt acatccgggg ccgtgctcat
     2461 cctgcctagg ccgaaccgaa tcgaggcccc gctccagtct ttggggactc ccatggcttc
     2521 gagcacgtag ctcggtcccg gctgcgcgct gctgcaggcc gagccgctcg agaccgaaac
     2581 cacctcggca agctcgagaa gcagccgcgc tccatcgagg tagccgaacg acaggttgag
     2641 gttgcctgga tgccgtggat cggcggcccc gttgagccgt agctcttcaa tctcggcgaa
     2701 gagctggttc ttcagggcgt cacgtagcga tccgatccgt tcggcttcag tggcgccttc
     2761 gttcttcatg atttctgcgg ccaggccgaa cccgacgatg cctggcacgt tgagggttcc
     2821 agaacgaaga ccacgttcgt gcccgcctcc gtggatctgc ggagccggga tgccgcgcgt
     2881 ctggagcgac tctcgaacat agagggcgcc gacgcccttc ggtccgtaca tcttgtgcgc
     2941 cgagaggctt gcgaggtcga tgtgcatctc gtcgagcgac cagggcagat acccgagccc
     3001 ttgtgccgca tcgcaatgga acgtgatgcc gcgtctgcgg gtaagtgcgc cgatggcgcg
     3061 caggtcctgg gcgacgccga cctcgttgtt tgcgagcatg acgctcacga gaacggtctc
     3121 atcggtgagg gcatccgcga gcgcgtccac cgagagcagc ccgttcgagt cgacatcgag
     3181 ccggctgatg cgccagcctt cccgttcgag cgcagcgcaa gtgtcgagca cggccttgtg
     3241 ctcggttcgg acagtcacga tgtgcttgcc gtctccgatg cgagcctcgg cgacaccacg
     3301 gacggcaagg ttgtcgctct cggtggcccc ggaggtgaag atcacctcct ccggcttcgc
     3361 gccgatcaga tctgcgacct gcgacctgcc ccgctcgacc gcgtctcgcg ccacccgccc
     3421 gtgggcgtgg gtacggctgg cggcgttccc gaatcgaccg ctgaagtacg ggagcatcgc
     3481 ctcgaccacc cgctcgtcga ccggggtcgt cgcctggtgg tccatataaa taggcatttt
     3541 gctcatcgca gaaccccacc ttagcgccga ccatgtgcgc ctccaacgtg ttgatctatg
     3601 ctcttaccat ggctggagcg agcatccttt gtgcaactga tctgacctct gcggccccgc
     3661 ggaccgtcga cctcgcggtc gcggcggcgc aagcgttcgg cgtgcggatc gatctggtcc
     3721 acgtgttcga cgacacggat tcctggtggc ccgagtcccc cgatctccaa gctgc
//
LOCUS       DASRZF010000326        12205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_5062181, whole genome shotgun sequence.
ACCESSION   DASRZF010000326 DASRZF010000000
VERSION     DASRZF010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 12205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12205
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5062181"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..377
                     /locus_tag="VFG22_14280"
     CDS             81..377
                     /locus_tag="VFG22_14280"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET6417461.1"
                     /translation="MSIDPSTPPWTFLSNHGHILLSIARDPNIRVREIARSVGITERA
                     VQRILGELEEAGVISRLRQGRRTHYEIDRSQPLRHPVEAAHSVGELLRLARSRR"
     gene            complement(383..1717)
                     /locus_tag="VFG22_14285"
     CDS             complement(383..1717)
                     /locus_tag="VFG22_14285"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HET6417462.1"
                     /translation="MTRTRPAIHCSIEAVGFLAFALLAIAPMHSLAQEPPPKEPEASP
                     ESVSEVAMEEAVVDSAAQEVLENLTTSPGGVPASAADAETAIEMVKLSMAAVERQELA
                     GIGDDYMIQFGDTFDWVRLVSGEWLKGTVKRMRDDTLEFDSDKLDMQHIDFADVTHIH
                     SPTVHTYAFDDRVSATGRAVVTPDKVIVETEEGTKTFARSEMESIVSGGEREKDWWSM
                     KLRFGLTLNRGNTNQLTFNVDFNTRREDELTLLDLNYNTSFGRTGGVQNVKRHLGELK
                     FKVFLSHRWFLAPLFGQLFNDRFQNIQFRATPASGAGVHIIDAPKVKWDFQTGVGYQY
                     LKYPEGSSISPDGKNPQNDFFIPLFTYADFDITGDIDLTLSWLTNLVVTTIGNTNHTG
                     KADLAIELNSVIDLDIAFLFFRTEDPAPSSDPDVTVYKNDYQLVIGVSLELG"
     gene            1845..2369
                     /gene="purE"
                     /locus_tag="VFG22_14290"
     CDS             1845..2369
                     /gene="purE"
                     /locus_tag="VFG22_14290"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HET6417463.1"
                     /translation="MTSSKLNKNDPPVVAILTGSPNDLPVVLKGRDVLDELDIPCDVR
                     VLSAHRTPRETAEYIAAAEKLGVEVFIACAGMAAHLAGVVAAHTTRPVIGVPIASGAL
                     QGVDALLATVQMPPGVPVATVGIDGAKNAAFLAARIVALSHPEVGKRLRDQLEESKKR
                     YDSPDPSTPGQAAT"
     gene            2411..3583
                     /locus_tag="VFG22_14295"
     CDS             2411..3583
                     /locus_tag="VFG22_14295"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET6417464.1"
                     /translation="MSLGRILIFLGVISGLTIGTHYYLWARLIRDPMWPSPWRQVATI
                     TLVVLALSIPASMMAWRTLPRNVAIPVSWVGYVWMGSMFLLLVLLWGGELARWSWVKY
                     ASMTSVNGGRRELLAQILASGVGVFGLALSGWGVWSAIRPVEVKRVSVRLKKLPGSLN
                     GLRLVQLTDMHIGLTIGRDFVEDVVRKVNALEPDIVAITGDLIDGSVEDLGAAVAPLG
                     EIEARLGTFFVTGNHEYYSGADSWLAFLGTLDIRVLRNEHVELSKDGEAIHLAGVDDW
                     TAHQFGNGHGSDMARAMAGRDASKPVILLAHQPIQFDEARAHGVDLQISGHTHGGQIF
                     PFGLLTRLAQPFLSGLHQRGDSQIYVSSGTGYWGPPMRIAAPAEITLIELDRDAAV"
     gene            complement(3580..5565)
                     /locus_tag="VFG22_14300"
     CDS             complement(3580..5565)
                     /locus_tag="VFG22_14300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417465.1"
                     /translation="MTWSAWASSLAFFLAGLFPLFTADAYGHLAQGRQIVALGRVPEV
                     DLFSFWKPTPQPWMNYEWAYDWLSWLLYDHLGASALVLSKCLALAALGYLLVLLADRL
                     ARSAALAAPFALIALLIALPVARFRFTARPQIIGLVFPAVLLMGIAALYGELSSPRRK
                     AWILAGLGLLQIVWVNSHGSHLFGVAVTLIFAVFAVRTHAFRWMIGLLLVQAAATACT
                     PFGASIASDAISHLIQPEYRDLVVEWAAWSSKDPLRLLLAPITVALFVLLSMRGVTRS
                     SRFGLAYGVLCVLLSLMAFRSMRFVAHQLLFCAPFIAAGIARLPGFERLRRSLPALVG
                     LAALASLLWTAQMQPSLGFGLGESKRGYPAASAEVIERNVENPRILASIEDSWFLMFE
                     APNGRLLIDGRVPFYGPEMMQRISRSFADADLFAQQVSEYELNTVVIDHTRSDHIAAT
                     EYLSGREDWALAFIEDGHSLFIRRDASHELEPFRILGAGYRAGRVLDASFSDSALGAE
                     VGRIGTQANTASIHAWHEGLALLRPLARAGDRAGTRMHRDPDERALARAAYERLGIPA
                     SSYPGFTSIELYRAMAALSACDVEEARIALGRARYGGQTRETSLLGLELGLRAGDAAE
                     RSKAMAHLVQLQAHPQSRSDPWVIAIAEDSAVRCAGP"
     gene            complement(5631..6536)
                     /locus_tag="VFG22_14305"
     CDS             complement(5631..6536)
                     /locus_tag="VFG22_14305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HET6417466.1"
                     /translation="MASPEKIAVTGASGLVGSALVSELRRKGIDVVRLVRRAPASKDE
                     VRWDPDAGTIDVAGLGGITGAIHLAGDNIASGRWTDAKKASIRNSRVRGTEVLAHALA
                     QLSPKPRVFVSASAIGYYGARGDQAVDESAAQGSGFLASVCGEWEAAADEARAAGIRV
                     VHPRIGVVLASEGGALAKMKLPFLFGIGGRIGDGAQYMSWIALEDLVSALIFALGRED
                     LEGPVNFVSPTPVTNADFSATLGRVLHRPAILPVPKFALRLGVGSEMADEMLIGGVRV
                     IPASLHAHGFRWEHTSLEPALRSLL"
     gene            6681..8027
                     /locus_tag="VFG22_14310"
     CDS             6681..8027
                     /locus_tag="VFG22_14310"
                     /inference="COORDINATES: protein motif:HMM:NF019924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HET6417467.1"
                     /translation="MATLAKVMRRADHAVDLVSPLYGHIDTAKLALARRLTKLEIELG
                     GRAWSCELYTSRNPDGVGLTFIGNEEVLLSAASLTGEGEQLDASRVGVFAAAVTELVR
                     QNASDFDLVHGHGWAAAAAIARLRTELPEMPTVLTVYDASKQGCFDEGDADSLGLGEE
                     ARTADGINVLKAGLLTADRITMLSPNYTHRLQRADSENPLSDVFREIAPKLDSIRHGL
                     DPAVWNPATDGHLPARFDPFDMEGKDKCKAELQRELVLPVRSDVPLFGAIGHVDDDGM
                     ALLAKIGPDFLRNDVQLVVQLDGDGELVAIYEELWDQWPDRIQIRTGNDEAFRHALIA
                     ASDFLVVPGMGAPSDNVQMRAQRYGALPIVHREGGLADEVVDCEASLKTGSGILYDEP
                     TTRSLLAALQRAAAAFANNKAFRDVQSRVMRMDHSWERSTRLYERAYREAIAAHTS"
     gene            complement(8024..8746)
                     /gene="tsaB"
                     /locus_tag="VFG22_14315"
     CDS             complement(8024..8746)
                     /gene="tsaB"
                     /locus_tag="VFG22_14315"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HET6417468.1"
                     /translation="MTTLCIETATEHGLVAIVRDDGSSAFARWRSASRHGENLFGYIE
                     RVLAELGATREELSLIGVDTGPGRFTSLRVGLGTAKGLALGLQLPIVGVSSLRVLARS
                     IEADPSVVRVPIMNAYRGDVFAAAYRFRDGVVEEISAPQFGPPERVFARIGALLDGNR
                     IALGGEGVEPHAAAAQKAFGVAFDESVRGPEAPSPEAIAAEVREVYRTAGPSDLDSLE
                     PQYLRPSDAKLPTQEQRTGRRS"
     gene            8835..9569
                     /gene="lepB"
                     /locus_tag="VFG22_14320"
     CDS             8835..9569
                     /gene="lepB"
                     /locus_tag="VFG22_14320"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET6417469.1"
                     /translation="MSNHQDKSASKGASKAKKGDGWKSNLLTIGGALLLALFIRVTLF
                     EAFAIDGPSMEPTLLDGDRVVVAKYPFGLFLPFTHEAVVNWGGPDPGDVIIVHSPADN
                     EDIVKRVIGVGGDEILIRDGKISRNGEVLETIDLGPCSPEEQKNLDPTCRVYQETLDG
                     DPHRISHSKHDFPLDRLPLRVPEGHVYVLGDHRDSSNDSRNPLVGAIPNSRVKGKSLF
                     MYMSWKNTPGSVWERIRWSRVGSSVD"
     gene            9597..11096
                     /locus_tag="VFG22_14325"
     CDS             9597..11096
                     /locus_tag="VFG22_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET6417470.1"
                     /translation="MSDPKGEILTRMADEIGAIQKDAPEAVALLYPSPYRVGMSSLGY
                     QTIYRSINARDGRAAHRAFLPDDLNAWEQTRVPLVTYETLRPVSDYRVIAVSVAYETE
                     LAGLIQCLELAGVPPLAEERGEQHPFILAGGPLTFSNPLPLAPFVDAVIMGEADQSVH
                     SALDLIFSSDSKASALRALGSQPHVFVPSMHGDMLPSIDKADASALPAFSQIVTPHTE
                     LRNMFLIEPERGCHRGCTYCVMRRSTNDGMRVIPMDRILSLVPEAAQRVGLVGAATTD
                     HPQIVDIVNALADSGREVGLSSLRADRLTDSLVAALRRGGHRILTTASDGASQRMRDI
                     IQRRTSEEQLRSAAKLARRHGMKRMKLYVMVGLPDETDDDIDELIEFGTELSSLIPVS
                     LGVAPFVAKRNTPLDGAPFAGMPLVEARLRRLRRGVRGRVDVRATSARWAWVEYVLAQ
                     GGRAEGRAVLEAVHGGGRFSDWKRAFKALPKDRPRRLLVRPGDRLGRAS"
     gene            complement(11103..>12205)
                     /locus_tag="VFG22_14330"
     CDS             complement(11103..>12205)
                     /locus_tag="VFG22_14330"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HET6417471.1"
                     /translation="SIFLGLETMSLGAYALVGYRRTSARAVEAAVKYFLLGSFAAAIL
                     LFGSALLYGATGHTDLAGIERVVAAGEANAILVILALVMLIVGLAFKLGAVPFHMWVP
                     DTYEGAATPVTTFMSVGVKAAAVAAMLRVLVGAFGDPVSMGLYTGWTPVIVLLAVATM
                     VYGNFAAIAQSSVKRMLAYSSIAHAGYILVGLAAVYQVGSFAVSAVLYYVAAYTVSNV
                     LAFGSLILMGSKGKEAVSFDDLAGAGRRHPLAAFPFVIGVLSLLGMPPTAGFFGKYYV
                     FSAAVQAGGPMVWLAVLGVLASAVGAYYYLKVIVYLYMREPADDQPIAVPMQSIYVVV
                     ALVLAGYYVVKMGITPSRYLDWAVSAASSFVG"
BASE COUNT         2367 a         3830 c         3913 g         2095 t
ORIGIN      
        1 gctgctccaa gcatgataat tttttcctgc ttgtcaatac atgttttctg ttttaggtaa
       61 tgagagacag gaaaatgtcg atgtcgattg acccaagcac cccaccctgg actttcctca
      121 gcaatcacgg gcacatcctg ctctcgatcg cccgagatcc gaacatccgg gtccgggaga
      181 tcgcgcgctc ggtcggaatc accgagcggg ccgtgcaacg cattctcggg gaattagagg
      241 aggcaggcgt gatttcgcgc ctccgtcagg gccgaagaac acactacgaa atcgaccgaa
      301 gccagcccct gcggcacccc gtggaggccg cccactcggt aggggagctt cttcgcttgg
      361 ctcgttcgcg acgctagatc gctcaaccca gctcgaggct cacgccgatg acgagctggt
      421 agtcgttctt gtagaccgtg acgtcagggt ccgacgaagg cgccggatcc tccgtcctaa
      481 agaagaggaa tgcgatgtcg aggtcgatca cgctgttgag ctcgatcgcg aggtcggcct
      541 tgcccgtgtg gttggtattg ccgatcgtgg tgaccacgag gttggtgagc caactcagcg
      601 tgagatcgat atcgccggtg atgtcgaagt ccgcataggt gaagagcggg atgaaaaagt
      661 cgttctgtgg attcttacca tcgggggata tcgagcttcc ctccggatat ttcaagtatt
      721 ggtatccgac gcccgtctgg aaatcccact tcaccttcgg cgcatcgatg atgtgcacgc
      781 cggctccgga agccggtgtg gcccggaact ggatgttctg gaaacggtcg ttgaagagct
      841 gaccaaacag cggcgcaagg aaccatcggt gcgaaagaaa caccttgaat ttgagctcgc
      901 cgagatgacg tttgacgttc tgcacgcctc cggtgcggcc gaacgaagtg ttgtagttca
      961 ggtcgagcag ggtcagctca tcttcacggc gggtattgaa gtcgacgttg aaagtgagct
     1021 ggttggtatt gcctcggttg agcgtgagac caaagcgaag cttcatcgac caccagtctt
     1081 tttctcgctc gccgcccgaa acgatggact ccatctcgct ccgagcaaat gtcttggtgc
     1141 cttcttctgt ttcgacgatg accttgtcgg gcgtgacgac cgcgcgaccc gtcgccgaaa
     1201 cgcgatcatc gaaggcgtac gtatggacgg tcggcgaatg gatgtgtgtg acgtcggcaa
     1261 aatcgatgtg ctgcatgtcg agcttgtcgc tgtcgaactc gagcgtgtcg tcgcgcatgc
     1321 gcttgaccgt gcccttgagc cactcgcccg agaccaggcg gacccagtcg aaggtgtcgc
     1381 caaactgaat catgtaatcg tcaccgatgc ccgcgagctc ctgccgctcc accgccgcca
     1441 tactgagctt caccatttct atggcggtct ccgcatccgc cgccgaagct gggacgccac
     1501 caggtgaggt ggtcagattc tcgaggactt cctgggctgc gctgtccacg acggcctctt
     1561 ccatggcgac ctcagaaacc gactctgggc tggcctcggg ctcctttggg gggggctcct
     1621 gcgcaagcga gtgcattgga gcgatggcga gcagcgcgaa tgccaggaag ccgacggctt
     1681 caatgctgca atggatagct ggccgagttc tagtcatggt tgcggctgtt tatagaagga
     1741 ccggcggaaa cggtaaaggc ctgccggtgc ggtttctggc ctctcacgga gacgggtgac
     1801 gtctggacgc ctttgagcta gaagctcggc gtttgggagg caaggtgacg agcagcaaac
     1861 tcaacaagaa cgatcctccg gtggtcgcga ttctcacggg cagccccaac gatctgcctg
     1921 tagtgctcaa gggccgcgat gtgctggacg agctcgacat cccatgcgac gtccgagttc
     1981 tctctgccca tcgcactccg cgggagaccg ccgagtacat cgcggcggct gaaaagctcg
     2041 gcgtcgaggt gtttatcgcc tgtgcgggaa tggccgcgca tctcgctggt gtagtcgccg
     2101 cgcacacgac tcgccctgtc atcggtgtgc cgatcgcatc aggtgcgctt cagggcgtcg
     2161 atgcattgct cgcgacggtg caaatgccgc cgggcgtccc ggtggcaacc gttggtatcg
     2221 atggagccaa gaacgcggcg ttcttggcgg cccgcatcgt ggcgctcagc catcccgagg
     2281 ttgggaaacg actgagggat cagctcgaag agagcaagaa acgttacgac tcgcccgatc
     2341 cctcgacgcc gggccaagcg gcgacttgac gcggtctgcg cggatcctta tgcgccttta
     2401 gtcgccgctc atgtcgcttg gccgcattct catcttcctc ggagtcatct ccggactgac
     2461 gatcggaacc cattattacc tctgggcccg tttgatccgc gatcccatgt ggccttcgcc
     2521 ctggcgccag gttgcaacga tcacgctggt ggtgctcgcg ctgtcgatcc cggcctccat
     2581 gatggcctgg cgcaccttgc ctcgtaacgt cgcgattccc gtctcgtggg tcggctacgt
     2641 gtggatgggt tcgatgttcc tcttgctcgt ccttctctgg ggaggtgagc tcgcgcgctg
     2701 gagctgggtg aaatacgcga gcatgacctc cgtcaacggc ggacgtcgcg agctcctggc
     2761 gcagatactc gccagtggcg tcggtgtgtt tggactggcc ctatcgggct ggggtgtctg
     2821 gtcggccatc cggccggtcg aagtgaagcg cgtctcggtt cgcttgaaga agctgccggg
     2881 ctcgctaaac ggactacgcc tggtccaact caccgacatg cacatcggtc tcacgatcgg
     2941 acgcgatttc gtcgaggatg tcgttcgcaa ggtcaacgcg ctcgagcccg acatcgtggc
     3001 gatcacgggt gatctgatcg atggctcggt cgaggatctg ggcgccgcgg ttgcgccgct
     3061 cggcgagatc gaggcgcggc tcggaacctt cttcgtcacc ggcaaccacg agtactactc
     3121 gggcgcggac tcctggctcg cgtttcttgg gactctcgac atccgcgtct tgcgaaacga
     3181 gcacgtcgag ctcagcaaag acggcgaagc catacacctg gcaggggtcg atgactggac
     3241 ggcgcatcaa ttcggcaacg gccatgggtc cgacatggcg cgcgccatgg cagggcggga
     3301 tgcgagcaag ccggtgatcc tactggctca ccagcccatc caattcgacg aggcgcgagc
     3361 acacggagtg gaccttcaga tctcggggca cactcacgga ggacagatct ttcctttcgg
     3421 gctgctcacc cggctcgcgc agccgttcct gtccggactt caccagcggg gcgactcaca
     3481 gatctacgtg agctcgggca cgggctactg gggcccgccc atgcgcattg ccgctcccgc
     3541 ggagatcacg ctcatcgagc tcgatcggga cgcagccgtt taagggcctg cacagcggac
     3601 ggcgctgtcc tcggcgatgg cgatcaccca gggatcgctg cggctttggg gatgcgcttg
     3661 aagctgcacg agatgtgcca tcgctttgga acgctccgcg gcatccccag cccgcagtcc
     3721 gagctcgaga ccgagcagcg aggtttcacg cgtctgcccg ccgtagcggg cgcgaccaag
     3781 cgcgatgcga gcttcctcga catcgcaggc ggagagcgca gccatcgctc ggtagagctc
     3841 gatgctggtg aagcctggat aactcgaagc cgggatgcca aggcgctcgt aggctgcccg
     3901 cgccagtgcc ctttcgtcag ggtcgcggtg catgcgcgta ccggcgcgat cgccagcccg
     3961 cgcgagcgga cgcaggaggg cgaggccttc atgccaagca tgaatggagg ccgtgttcgc
     4021 ttgcgtaccg atccgtccga cctccgcgcc gagggccgag tcggagaagc tcgcatcgag
     4081 gacccgcccg gcacggtatc ccgcacccag aatgcgaaac ggctccagct catgggacgc
     4141 gtcacgccgt atgaacaggg agtgcccgtc ctcgatgaaa gcgagcgccc agtcctcgcg
     4201 gccgctcagg tactcggtcg cagcgatgtg atcggagcgt gtgtggtcga tgacgacggt
     4261 gttgagctcg tactcgctga cttgctgggc gaagagatcg gcgtcggcaa agctccgcga
     4321 tatgcgttgc atcatctccg ggccgtagaa tgggactcgg ccgtcgatga gaagccggcc
     4381 gtttggagcc tcgaacatca gaaaccagct atcctcgata ctcgcgagaa tcctggggtt
     4441 ctcgacattg cgctcgatga cctcggcaga cgctgcgggg taaccacgct tcgactcgcc
     4501 aaggccaaaa ccgagggagg gctgcatctg cgcggtccaa agcaagctcg cgagcgccgc
     4561 caggccgacg agcgcgggca gacttcgccg caggcgctcg aagccgggca gtcgtgcaat
     4621 gccggccgcg atgaacggag cgcagaacag caactgatgt gcgacgaatc gcatcgaacg
     4681 aaaggccatc aaactgagca agacgcaaag cactccgtac gccagaccga agcgagatga
     4741 gcgggtcact ccgcgcatcg agagcaggac gaacaaagcc acggtgatag gcgccagcaa
     4801 caagcgaagg gggtctttcg acgaccatgc cgcccattcg acgacgaggt ctcggtactc
     4861 gggctgaatc aagtgcgaaa tcgcgtccga cgcgatcgaa gctccaaatg gggtgcatgc
     4921 ggtggcggcg gcctgcacga gcaacaaacc gatcatccag cgaaacgcat gagttcgaac
     4981 cgcgaagacg gcaaagatga gcgtgactgc gacgccgaag agatgggagc catgggagtt
     5041 cacccagacg atctgcagca agccgagccc cgcgaggatc caggcctttc ggcgcggtga
     5101 gctcagctcc ccgtagagcg cagcgatgcc catcagcaga actgcaggaa acactaagcc
     5161 gatgatctgc gggcgcgcgg tgaaccgaaa gcgcgccacg gggagcgcaa tcaggagcgc
     5221 gattagggcg aagggcgcgg caagcgcagc gctccgggcc aggcggtcgg ccaagagcac
     5281 cagcaggtaa ccgagtgctg cgagcgcgag gcacttcgag agaaccagcg cgcttgcgcc
     5341 gagatggtcg tacagcagcc agctcagcca gtcataggcc cattcatagt tcatccaggg
     5401 ctgaggcgtc ggcttccaaa aggagaagag atcgacctcc ggtacccgtc cgagcgcgac
     5461 gatctggcgg ccctgagcca gatgaccgta agcatccgcc gtgaagagcg ggaacaagcc
     5521 cgcaaggaag aacgccaggg acgacgccca ggcgctccat gtcaccgtcc ccttcggcac
     5581 ggagctgagt gtccgtcgaa ggccgacgaa gggtcaaaca aggcgcagcg ctacaagagc
     5641 gaccgaagcg ccggctcgag gctcgtgtgc tcccaccgga agccgtgggc atggagcgaa
     5701 gcgggaatga cgcgcacccc gccgatcagc atctcgtcgg ccatctccga cccgacgccg
     5761 agacggagcg caaacttcgg cacggggaga atcgctggcc ggtggagaac acggccgaga
     5821 gtcgcggaaa aatctgcgtt ggtgacgggc gtaggcgaga cgaagttcac aggaccttcg
     5881 aggtcctcgc gcccgagcgc gaagatgaga gccgagacca aatcctccaa ggcgatccag
     5941 ctcatgtact gagctccgtc accgatgcgg ccaccgatgc caaagagaaa cggcagcttc
     6001 atcttggcga gggcgccgcc ttcggatgca agcacaacgc cgatccgggg gtgcacgacg
     6061 cggatacccg ccgcgcgggc ctcgtcggca gccgcctccc attcgccgca gaccgatgcg
     6121 aggaagccag agccctgggc agcgctctcg tcgaccgcct gatcgccgcg cgcgccgtag
     6181 tatccaatcg ccgaagccga aacgaacaca cgtggttttg gcgaaagctg agcgagcgcg
     6241 tgggcgagga cctccgtgcc acggacacgg ctgtttcgaa tgctcgcttt cttggcgtct
     6301 gtccatcggc cagacgcgat attgtcgcca gccaagtgga tcgcccccgt gatgccaccg
     6361 aggcccgcga cgtcgatggt ccctgcgtct gggtcccagc ggacttcatc tttggaggcc
     6421 ggcgcgcgcc tcacgagacg gacgacgtcg attcccttgc gccggagctc cgaaaccagc
     6481 gcgctcccaa cgagccctga ggctccagta actgcgattt tctcgggact tgccattggg
     6541 gataagcgtg gaccagcctg cttgcggcgc aagcaggctt tccacgtggc cggggagccc
     6601 aacactcgct atggtccagc actttgcgaa ttctcttcat tgcccctcaa gccttcaccc
     6661 cgagtcgaga tgcccaggaa gtggcaacct tggcaaaggt gatgcggcgc gcggaccacg
     6721 ccgtcgacct ggtatcaccc ctctacggtc acatcgacac cgcgaagctc gctctcgccc
     6781 ggcgtctcac caagctcgag atcgagctcg gcggcagggc ctggtcgtgt gagctctaca
     6841 cgagccgaaa ccccgatggt gtaggactga ctttcatcgg taacgaggaa gtcctcttgt
     6901 cggcagcatc cctcaccggc gaaggcgagc agctcgatgc atcgagagtc ggtgtctttg
     6961 ccgctgctgt gacggagcta gtccgtcaga acgcgagcga cttcgatctg gtgcacgggc
     7021 acggctgggc ggcggcggcg gccatcgcac ggcttcgcac agagcttccc gagatgccta
     7081 cggtcctcac ggtgtacgat gcttcgaagc agggatgctt cgacgagggc gatgcggact
     7141 cgctcgggct tggcgaggag gcgcggaccg cggacggaat caacgtcttg aaggccggtc
     7201 tgctcaccgc cgaccgcatc acgatgctct cgccgaacta tacacaccgt ctgcaaagag
     7261 ccgacagcga gaacccgttg agcgatgtgt tccgggaaat cgctcccaag ctggacagca
     7321 tacgccacgg actcgacccg gcggtctgga atcccgcaac ggacggacac cttcctgctc
     7381 gcttcgatcc tttcgacatg gagggcaagg acaagtgcaa ggccgagctt cagcgcgagc
     7441 tggtgctgcc cgtccgaagc gatgtgcctc tattcggtgc catcgggcac gtggacgacg
     7501 atggcatggc cctcctggcg aagatcggac ctgactttct gcgcaacgac gtccaactcg
     7561 tcgtacagct cgacggcgac ggggagctcg tggcgatcta cgaggagctc tgggaccagt
     7621 ggcccgaccg catccagatc aggacgggca acgacgaggc atttcgtcac gcattgatcg
     7681 cagcgtccga cttcttggtc gtgcctggaa tgggagcccc ttcagacaac gtccagatgc
     7741 gagcgcagcg ctacggagcg ctgcccatcg tgcaccgcga aggcggactc gcagacgaag
     7801 tggtggattg cgaagcctcg ttgaagaccg gctccggaat tctctacgac gagccgacga
     7861 cgagatctct gctcgctgcg ctacagagag ccgcagccgc gtttgccaac aacaaagcgt
     7921 tccgcgacgt gcagagccga gtgatgcgca tggatcattc ctgggaacgc agcacgcgct
     7981 tgtacgagcg agcttaccgc gaagcgatcg ccgcgcacac gagttagctc cggcggccgg
     8041 ttctctgctc ctgggtagga agcttggcat cgctgggccg cagatattgc ggttcgaggg
     8101 agtcgaggtc ggagggtccg gcagtgcggt agacctcgcg gacctcggct gcgatcgctt
     8161 cgggcgacgg cgcttcgggc ccgcgcacgg attcgtcgaa ggccacgccg aacgccttct
     8221 gcgcggcagc ggcgtgcggc tcgacgccct ccccgcccag cgcgatccga ttcccgtcca
     8281 gcagggcccc aattcgagca aacacgcgtt ccggtgggcc gaactggggc gctgagatct
     8341 cttcgacgac gccgtcccgg aaccggtatg cggccgcaaa gacgtctcct cgatatgcgt
     8401 tcatgatcgg aacccgaacg acgctcgggt cagcttcgat cgaccttgcg aggacccgca
     8461 gcgagctgac cccaacgatc ggaagctgga gcccgagagc caagcccttg gccgtgccga
     8521 gtccgacacg caagctggtg aatcggccag gccccgtatc gacgccgatc agcgaaagct
     8581 cctcacgcgt cgcgccgagc tccgccaaaa cgcgctcgat gtaaccgaag aggttttcgc
     8641 catgacgaga agccgagcgc cagcgtgcaa aggcggacga gccgtcgtcg cgcacgatcg
     8701 cgacgagccc gtgctccgta gcggtttcga tgcagagagt ggtcataggg tcgcttgacg
     8761 catgcctacc cgcagactag cgtccgcgcc agcgaagcgt ctgcttgcgc cccttgctgg
     8821 caagtcccgc acgaatgagc aaccaccaag acaaatcggc ttcgaaaggg gcgagcaagg
     8881 cgaagaaggg ggacggctgg aagtcgaacc tgctcacgat tggcggcgcc ttgctcttgg
     8941 cactgttcat ccgtgtgacc ctattcgagg cgtttgcgat cgacggccct tcgatggagc
     9001 cgacgctgct cgatggcgac cgggtcgtcg ttgcaaaata cccgttcggt ctgttcttac
     9061 cgttcaccca tgaagccgtg gtcaactggg gtggcccaga tcccggcgac gtgatcatcg
     9121 tccacagccc ggccgacaac gaggacatcg tcaagcgtgt gatcggcgtg ggcggagacg
     9181 agatcctcat ccgtgacggc aagatctccc gaaacggcga agtgctcgaa accatcgact
     9241 tgggaccgtg ctcgccagag gagcagaaga acctcgaccc gacgtgcaga gtttaccagg
     9301 aaaccctcga cggcgacccg caccggatca gccattcgaa gcacgacttt ccactcgacc
     9361 ggcttccgct tcgcgtccct gaagggcacg tctacgtcct cggcgaccac cgcgacagct
     9421 ccaacgacag ccgtaatccc ttggtcggcg cgattcccaa ttcgcgtgtc aagggcaagt
     9481 cgctgttcat gtacatgtcc tggaagaaca cccctggctc agtttgggaa cgcatccgct
     9541 ggtcccgcgt agggagcagc gtcgactaac cctccgcacc gccctaggct tgcgctgtga
     9601 gtgatccgaa gggagagatc ctgacgcgta tggccgacga gatcggggcg atccagaagg
     9661 acgctccaga ggcggtggcc ttgttgtatc cgagcccgta tcgggtcggg atgtcgtcgc
     9721 tcggctatca gacgatctac cgctcgatca acgcgcgcga cggtcgtgcc gcccatcggg
     9781 cctttctgcc ggacgatttg aacgcgtggg agcaaacgcg cgtacctctg gtcacttatg
     9841 aaacattgcg gccggtttcc gattaccggg tgatcgccgt ctcggtggcc tacgagaccg
     9901 agctggcggg gctcattcag tgcctcgagc ttgcgggcgt ccccccactg gccgaggaac
     9961 gtggagaaca gcatccgttc attctcgccg gtggccccct caccttcagc aatccacttc
    10021 ccttggcgcc gttcgtcgac gccgtgatca tgggcgaggc ggaccaatcg gtgcattccg
    10081 ctctcgatct gatcttcagc tcggactcca aggcaagcgc cctgcgcgcg ctcggcagcc
    10141 agcctcacgt cttcgtgccg agcatgcacg gcgacatgct cccatccatc gacaaggcgg
    10201 acgccagcgc cctgccggcg ttctcgcaga tcgtcacgcc gcacaccgag cttcgcaaca
    10261 tgttcctcat cgaaccggag cgcggatgtc accgaggctg cacctattgt gtgatgcggc
    10321 gctccacgaa cgatggcatg cgcgtcatcc cgatggatcg aatcctctcg ctggttccgg
    10381 aagcagccca gcgtgtcggt ttggtgggcg cggcgacgac cgaccatcct cagatcgtcg
    10441 acatcgtcaa cgcgctcgca gactcgggac gagaggtggg cctttcgtcg cttcgcgcag
    10501 acaggctgac cgattcgctc gtggcggcgc ttcgccgtgg tgggcaccgc atcctcacga
    10561 cggcgagcga cggtgccagc cagcgcatgc gtgacatcat ccagcgacgc accagcgagg
    10621 aacagcttcg aagcgccgcc aagctcgcgc gacgccacgg tatgaagcga atgaagcttt
    10681 acgtcatggt gggtctcccc gacgaaaccg atgacgacat cgacgagctg atcgaattcg
    10741 gcacggagct ctcgtcgctc atcccggttt cactgggagt ggcgcccttc gtcgcgaagc
    10801 ggaacacccc gctcgacggc gcgccctttg cgggcatgcc cctggtggag gcgcggcttc
    10861 ggcggctaag acgcggggtt cgaggccggg tggacgtgcg cgcgacgagc gcgcgctggg
    10921 cctgggtcga gtacgtgctc gcgcaaggcg gacgtgcgga ggggcgagcg gtgctggagg
    10981 cggtgcatgg aggcggtcgt ttctccgatt ggaaacgcgc gttcaaggcc ctgccgaagg
    11041 atcgtccccg gcgcctgcta gttcgcccgg gcgaccgcct gggtcgcgcg agctagcgca
    11101 ggctagccga cgaagctgga cgccgccgag acggcccagt ccaagtagcg gcttggggtg
    11161 attcccatct tgaccacgta gtacccggcg agaaccagcg cgaccacgac atagatagat
    11221 tgcatgggaa ccgcgatggg ctggtcgtct gcaggctccc gcatgtagag gtagacgatg
    11281 accttgaggt agtagtaagc gcccaccgcg ctcgccagga cgcccagcac cgcgagccat
    11341 accatcggac cgccggcctg cactgcagcg ctgaacacgt agtacttgcc gaagaaaccc
    11401 gcggtcggcg gcatgccgag caaggagagg acgccgatga cgaagggaaa cgcagccaag
    11461 ggatgccgac ggccagcgcc cgccaggtcg tcgaagctaa ccgcctcctt gcccttcgag
    11521 cccatgagaa tgagagagcc gaacgcgagc acgttggaga ccgtgtacgc cgcgacgtag
    11581 tagagaaccg cgctgacggc gaaggatccg acctggtaga cggcggctag gcccaccaga
    11641 atgtagcccg cgtgcgcaat cgaggaatac gccagcatgc gcttcacgct gctctgcgcg
    11701 atagcggcga agttcccgta aaccatggta gcgacggcga ggagcacgat cacgggcgtc
    11761 cagccggtat aaagacccat gctgaccggg tcaccgaacg caccgaccag aaccctcagc
    11821 atggccgcaa ccgcagccgc cttgacgccc acggacatga acgtggtgac cggcgtggcc
    11881 gcaccctcgt aggtatcggg cacccacatg tggaacggaa cggcgccgag cttgaacgcc
    11941 aggccgacga tgagcatgac cagggcgaga atgaccagga ttgcgttggc ctcgccggcg
    12001 gctaccaccc gctcgatgcc agccaagtcg gtgtggccgg tcgcgccata gagcagagcg
    12061 ctgccaaaga gcagaatcgc tgccgcgaaa gagccgagca ggaaatactt caccgcggct
    12121 tctactgcgc gggcgctggt acgccggtag ccgaccagcg cgtacgcgcc gagcgacatc
    12181 gtttcgagcc cgaggaagat gctca
//
LOCUS       DASRZF010000327        33986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4040226, whole genome shotgun sequence.
ACCESSION   DASRZF010000327 DASRZF010000000
VERSION     DASRZF010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 33986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33986
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4040226"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="VFG22_14335"
     CDS             <1..726
                     /locus_tag="VFG22_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417472.1"
                     /translation="QSRKVQYLTRRIEDAVTAWTGAILNGLLRDAEKEALSIDLYDAS
                     FRPDNDHQGLYEWERQWFRRRLPGPPASILIGAAGAGREACALRELGYRVHAFEPSTS
                     AFRVCQIKVGSEFVDQASYQDLVATVLRGESTRLRLANEAKFDAVLLGWGSFGHVLQR
                     ADRLELLRACDLLAPNGPILLSIFEPNATASTNGTYYMPWGGFLVQPTPEELDQHAEA
                     LNRQLVMSLQAPSPYATLIPNGY"
     gene            complement(671..1570)
                     /locus_tag="VFG22_14340"
     CDS             complement(671..1570)
                     /locus_tag="VFG22_14340"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET6417473.1"
                     /translation="MIARVDHAAQDRLRTTLIGELALSLANQVFEACAERGIVLMPLK
                     GAFLLARWPALRGRRDLVDIDLLVRSSDIEAVALVLHALGFEATVDSSAGSTFASDLW
                     PLSIDVHHHLFPHGLFGMSTDDVFSRAAIDASLFAAPVARMSDQDLFAHLIGHFVKGR
                     GTFRQDTSLDDIRWLLKENLYRLEDAETLGGHFRALGLQRAAGYVLGHESFRDDPIAC
                     AANRALELSRWDRILIAMVRLDTSANAGSPRWWTPHLLDRSLVAGSRSLVAHAYEAAR
                     RVLTRFSSRWGSKWRTGSAPEAT"
     gene            1685..2368
                     /locus_tag="VFG22_14345"
     CDS             1685..2368
                     /locus_tag="VFG22_14345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417474.1"
                     /translation="MATHQRLGDLAEALEAEGADELRVHVVRRAREFKRSWVNMAEAL
                     VEVRNRESYLDWGYEDFYSYCSLELQLKQATADKLTGSYVALTRHAPSVLKRDGLNER
                     IPTCDAVDYFAKALQRNPSNDADGERGVRQEVVDQLREAVFEEGAPVAELRKRFNPVF
                     SPKPEGAEYLDGIRRATATARRLEKMLEEIDGLPRPTVRAALDSLEALREDLTQLLER
                     TKAQYAKSA"
     gene            2409..2858
                     /locus_tag="VFG22_14350"
     CDS             2409..2858
                     /locus_tag="VFG22_14350"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HET6417475.1"
                     /translation="MTEKTLAPAVEGLFTMDPREPHLLGTRCRACGTYFFPAERTFCR
                     NPACDEADLEEVPLSRTGKVWSYTSASYKPPAPFVAKEPFEPFAIAAVELEAEGITIL
                     GQVADGIGVDALKTGMPMELVLRELYQDEEHVYLTWNWKPGDDGGAA"
     gene            2855..4042
                     /locus_tag="VFG22_14355"
     CDS             2855..4042
                     /locus_tag="VFG22_14355"
                     /inference="COORDINATES: protein motif:HMM:NF004715.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HET6417476.1"
                     /translation="MSKDVAVLGVGMHPWGKWGQSFVGYGVKAAQDALKDAGVQWRDI
                     QLVAGGDTIRNGYPGFVAGATFAQALGWSGAKVSSSYGACATGAQAIGVARAHILAGL
                     SEVALVIGADSAPKGFFAPTGGRRPEDPDWLRFHIQGITNPTYFGLYARRRMDLYGAT
                     QQDFAQVKVKNSKHGLHNPNARYRKEVALEDVMGSVLVADPLHLLDICATSDGAAALV
                     LSSMDFARKRTTNPVKIAAVSTVTPVYPNDVIEMPNLATDSKAGVGLPELGFKESIAH
                     RAYEEAGLGPEDMDLAEVYDLSTALELDWYEQIGLCPPGDAEKILRDGDTRIGGRVPV
                     NPSGGLSCFGEAVPAQAIAQVCELTWQLRGQAEGRQVEGARAGVTVNQGLFGHGSSVI
                     CTI"
     gene            complement(4044..4496)
                     /locus_tag="VFG22_14360"
     CDS             complement(4044..4496)
                     /locus_tag="VFG22_14360"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HET6417477.1"
                     /translation="MLRTASALFLLLALGCAPVPVDEAPPESRQVQTIVRNTLSPFCP
                     GKTLDSCPSPKAGEWRRDIRSWVAEGVPEPEIRTRLQERVPEFDLRIPPVKSGWVIPL
                     VAVVLSTLWLIIMALKFRGQGSSQRGAEARPWESNLDARLDEELARLD"
     gene            complement(4576..5877)
                     /locus_tag="VFG22_14365"
     CDS             complement(4576..5877)
                     /locus_tag="VFG22_14365"
                     /inference="COORDINATES: protein motif:HMM:NF019285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HET6417478.1"
                     /translation="MRSMMSDLGSVPFWQRLVSLAGLLAMVGIAWLLSRHRDRVPWRV
                     VSWGIGLQVSFGVFVMKTDLGLRVFAILNDMMLALLGFTAKGTEFVFGDFASEKFTIA
                     INVLPTLIFFSSLMTILYYLGIMQRLVGLFAWVMRRTMGTSGSETLSAAANTFVGQTE
                     APLMVAPFVRTMTISELMAVMTGGFATVAGGVLAAYVGLLKGVFPDIAGHLIAASVMS
                     APAALVIAKVMCPETEVSETAGSLGVEIDQPDANVIDAAARGAYEGMKLALNVGAMLL
                     AFIALIALANALIGLPFGLYNDWMGLEGSAAIEPLTLQRILGWLFWPFAFLIGVPAAE
                     CASVGTLLGEKLVLTEFIAYLHLYESLSEGVAALSPRTVVITSYALCGFANFGSIGIQ
                     LGGIGGIAPERRHDIARLGLRAMFGGLLASLMTAAVAGMLL"
     gene            complement(5915..7183)
                     /locus_tag="VFG22_14370"
     CDS             complement(5915..7183)
                     /locus_tag="VFG22_14370"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET6417479.1"
                     /translation="MSSDVITSNFERAYRPAAIALANRIGRALSRVGIGGKPITVTGV
                     LDDARRKVGLRDFGDEGFLEPLEILVDSINREAALNPVGHMIIRGRIVGVLVNKLVAQ
                     NTIERHPEILEIPVEAPIVVAGLARTGTTMLHRLIAEDPGIRSLASWEAINPAPAKPK
                     RLGKRDPRFAQAAAAAKGLKYMSPGFFAIHPAEPDAPEEEVILLEQAFLTTTPEAMMN
                     VPSYSKWLEEQDHVPAYQALKRMMQYLQWQRPGIGHDLRWVLKTPHHQEYFDPLLEVF
                     PNATIVHTHRDPLKTSPSLFSMLTHLQMIFSDEVDPNRVAEHWLYKIENMARRTMATR
                     ERVNDEGFVDVSYYDLVVDPIPEVARVYEAAGHELSSEALAAMNASRKINKQHKYGRH
                     SYSLADFGMTRDDVESRIAPYRARFKVPYE"
     gene            complement(7192..8292)
                     /locus_tag="VFG22_14375"
     CDS             complement(7192..8292)
                     /locus_tag="VFG22_14375"
                     /inference="COORDINATES: protein motif:HMM:NF018452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HET6417480.1"
                     /translation="MTDELSKEEREILDGTVWNRFCDTLKMAGNVVLGPNTPSDPMNR
                     MEGFRYLSRITRAALQTFVEHNDPLAPVLQRVVHETAKMGADHPDNFYQNAAISGEHE
                     YRIVGNRGSVHYLGFFTQTGNYGQGRGMPPTGYLEASTMKIEPDGSFEIILSSEKKPG
                     NWLPMTRETGTLIVRQTFLDRETEKLAELRIERIRGDGQPSPFDPVKCAEGLTTSAGL
                     VVGAAIMFASWAENFKKHTNELPRMPQEVSNQAGGVPDIAYYHSYWKLAPDEALVIDA
                     MPPDCDHWNFQLNNYWMESLDYRYYPIHVNKHTAKYRPDGSVQVVVAAKNKGFDNWID
                     TVGHEEGTMCWRWVRANENPQPQTRVVKLSEL"
     gene            complement(8302..9315)
                     /locus_tag="VFG22_14380"
     CDS             complement(8302..9315)
                     /locus_tag="VFG22_14380"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6417481.1"
                     /translation="MGNRTHDNPVAAVLSGLLDQRSKEPKVEAVAESVRLDGRTCLVT
                     GANSGLGKAIAIGLAKRGARVIMACRSGIPEAGEDVRAESGSDQVVMMEVDLSDFDSI
                     AAFCDRLRDENVTLDVAVFNAGVVPVTARTNKHGLELMFAVNFLAKFVLLNRFLHDGV
                     IPNAVYGNNSRAEDPPRIIFVSSETHRSSIPIDFEAFGAPVQYGVADGVKYYGLSKLH
                     LTTYFHELSRRLNPGSNGEDPDVCVHALCPGAVNSNMAREAPAWLKPILKPVMALFFQ
                     SPEKASIPADYLAVSEEMGHKTGEYMHMMRIKESSAASMNPANGKLLWSKTEDLLRRY
                     GVI"
     gene            complement(9349..10089)
                     /locus_tag="VFG22_14385"
     CDS             complement(9349..10089)
                     /locus_tag="VFG22_14385"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HET6417482.1"
                     /translation="MVRLSVNVNKVATLRNSRGGAVPSLLEAVRACVDAGAPGITVHP
                     RADERHIRPSDVYEIAEQLEPMRDRVEYNIEGDPRPALVELVEQVRPHQCTLVPVRPG
                     ELTSEAGWPAETPKSELIEIVERFQRHGVRVSVFVDPEPAPIDWAAELGADRVELYTE
                     PYARAFEQGGEVLVASLERYRRAIERATARGLGINAGHDLDLTNLPTFLTLSDIDEVS
                     IGHALMARALFVGLDRVVAEYLQITGSR"
     gene            10231..11016
                     /locus_tag="VFG22_14390"
     CDS             10231..11016
                     /locus_tag="VFG22_14390"
                     /inference="COORDINATES: protein motif:HMM:NF019463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417483.1"
                     /translation="MSAKAVVLEVLSAGESIYDGGLPVRSLVKAASVFDIAENSVRVA
                     IVRLRAEGLLESPGRGEYRLGPSAQVVNQKIHGWRTVSARVGSWDGSWTGVFTADLSR
                     TDRPALRRRLRALRFYGFEELKPGLFLRPNNLKPGIDGIREELVPLGLDADALVFRMD
                     ELDSDQERQARAMWNGASLEKNYAKLEGNLKLSMERLDKLPVDDSVREAFLLGREGIR
                     QVVLDPLLPGPLVDETRRASTVEVLKSYCDKGLNLWARFLQID"
     gene            11046..11978
                     /locus_tag="VFG22_14395"
     CDS             11046..11978
                     /locus_tag="VFG22_14395"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6417484.1"
                     /translation="MKSADFAFQTIDGANLHVSGWTVEQPKAIVQILHGMAEYGSRYA
                     RLAGALASAGYSTYAHDHRGHGRSIPVGSEPGHAADSDSWNRVVEDAHGVNREIAKRH
                     PGIPIIILGHSMGSFVLQQLLFEHPNDMVAAALSGSNGKPPAIAVLGKLIARLERLRV
                     GKRKPSPLLQRLTFGEYNKPYAPARTEFEWLSRDPGEVDKYVADPLMGFAVSTQTWLD
                     LLNALDRISNPSNIAKVPKGMPLYLFAGDQDPVGDKGKGMKNLYDAYKRAAIFDVRLK
                     LYPEGRHEMLNETNRQEVMDDFIAWCDEIIGARS"
     gene            complement(11975..12874)
                     /locus_tag="VFG22_14400"
     CDS             complement(11975..12874)
                     /locus_tag="VFG22_14400"
                     /inference="COORDINATES: protein motif:HMM:NF013978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis-aminopropyl spermidine synthase family
                     protein"
                     /protein_id="HET6417485.1"
                     /translation="MYEGAFRILRALGDGRSLSRDELVHTAGEASSVVREVTDRYPKW
                     FEIDERRIRCSECGLAALARELVARGSGQPRDASWRTAYEEIAAERATPKRALDQFYV
                     TTDTAVRRAELLLEAGEHQRGLVFLGDDDLTSAAMQLAGAARKVTALDSDEEILEVLR
                     RNAEKRAWELATVSHDLRQPIPKAMRARYGAVFTDPPYALEGFRLFLSRAVDLLKPDG
                     RLYVCFGQSRRASERGVQKQRALGDAGFLIEAVIEDFNEYHGAEAVGSRSALWRCRMT
                     PETSPLLRGEVEGPLYTSRTPDP"
     gene            complement(12883..13161)
                     /gene="infA"
                     /locus_tag="VFG22_14405"
     CDS             complement(12883..13161)
                     /gene="infA"
                     /locus_tag="VFG22_14405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HET6417486.1"
                     /translation="MARQGPRGRKRKFDRNFEQEDKRDDHIKLSGVVSQVYAGGQFEV
                     TTDSGAAVRAQLCGRMRKFRIRVILGDRVTVALSPYDLTHGMIVFRDK"
     gene            13297..13833
                     /locus_tag="VFG22_14410"
     CDS             13297..13833
                     /locus_tag="VFG22_14410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417487.1"
                     /translation="MVSIPVLQRHPDDGWALADPMKVGAPMRRVLLKLADAILPPNPR
                     LETTLEDVTRHALICLRYMPRSSALLFLLAMRVLNWSPLWRLRGFRPLTSLTSDAARR
                     QLVSASQSRWLPVRLLMYGPLGLFMSSYFDQDYVHRELGYAPVPFITGRIELRRAWVE
                     GQEPGADQEIRHLPVRAP"
     gene            13830..15335
                     /locus_tag="VFG22_14415"
     CDS             13830..15335
                     /locus_tag="VFG22_14415"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HET6417488.1"
                     /translation="MTILAHRDYRKLEEHQAEIVVIGTGAGGAVVGAELAEAGRDVLF
                     LEEGGYHPTSSFNPYLTESLPRLYRDAGGTIIMGRPPIPFAEGRCVGGSTVVNGGMAW
                     RPPETVLADWERITRQPDLGLKAMEPLLERVEERIHAKPQHASSIGEDSRLLEEGARR
                     LGWDYAHNRRAQDRCVGSNQCLTGCPTGAKQSTLLSYMPRALSTGARCLTEVRAQRLW
                     IERGRCVGVFGKAINPRTREEDVDVRVRAKAVVVACGAIQTPFLLLGHRDGRPSKQLG
                     KNFLCHPNAKVIAIYPDPIYAWQGVSQGGQIREFRNEGILMAENAVPPGTTAAQIPFL
                     GQEALDLMKRYDSMVMTGVLVEDSHSGTVARRFGGPMARYSITRYDHARFLRGVQHLA
                     TLHFEMGAERVILPFSNFHIARHVDELAGIERSQGSRSTIELFTVHMMGTCRMGSRPS
                     HSVVNLSGEVWDLPGCYVADASLFPTAVGVNPQVTIMALATRVAQRLQVPN"
     gene            15372..16376
                     /locus_tag="VFG22_14420"
     CDS             15372..16376
                     /locus_tag="VFG22_14420"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HET6417489.1"
                     /translation="MLDLPRLKRIRLMKRPIGQVFFGHTVLGPNYRFLPGIGIDIAGI
                     DKIPNEPVIYAMNHTDRFNYFPFMYEMWKKKERYITVWVKGKYYENPIVGTFMELTSN
                     LPTVSRGYIITKDFALTVGRRPTSAEYESLRVLVNEEAAPNGDSRTVDLSGIPRELLE
                     TKRDILGVPFDPGSCSYAVGINRVFTAMMAEFVRLNERSFELGLDLLVFPQGTRSIRL
                     PRGHIGMMEVALRYKKTIVPVGCNGCDLVYTGSLPIGKAGKIVYRVGEPIRYEELAEF
                     HIDEDFVPFSAEAEHKHRDKFQGAVDLVMNRINDLLDPEYQFSDDLESTGVRGTSRFI
                     "
     gene            16420..17757
                     /locus_tag="VFG22_14425"
     CDS             16420..17757
                     /locus_tag="VFG22_14425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417490.1"
                     /translation="MDTLQALRDATEPKPVVAIIGGAVAGSEAAAACAARGAIAVVFE
                     QGARPYGKIEDGLPRWHEKLRDKEYKKIDANLGSPGVIFVPKTEIGGDLKWSELRDEL
                     GFSAIVLANGAWSDRGLPVEGANHYEHRGLVFQNELVYWFNHYEEQGYQGPVFEIPDG
                     TIVIGGGLASIDVVKIVNLELYARALRARGIEVDIVEMEIKGIERTLEAHGLTAEELG
                     VKGCTLYYRRRKEDMPLASADNPTPEQLAKLQAARVKIMDRVIRKYFVKFEELSVPVG
                     MLLEDERLVGIRFQKTELVGGRVRALEGSEFDVRAPMIISSIGSIPRPIDGVPTKGEL
                     YHYDSWETGEVHGLPGVFGLGNVLTGKGNIKDSRANAIEIIEKVVAAYLGVGDSPEEA
                     PDLPSADAEALAERAVSGVPMSAEAMRRLAAKIEERHEAIGYEDYSGWIQASA"
     gene            17809..18834
                     /locus_tag="VFG22_14430"
     CDS             17809..18834
                     /locus_tag="VFG22_14430"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET6417491.1"
                     /translation="MLKLSPAVIDLTEFVYDLDVPREDWVPALLETGLPLFDHGHGVL
                     ATTYIRPLEGGLPVPTKFYQRTGPSDLAERIFVAAYEADEATLRDINRSGVVTTLSEE
                     QHRIPGGLKTFERYLAPAKDLLAITAVDPNGMGLSLSILLPEVTQLTGRDREIWKMVG
                     AHLVSGFRLRQSIEEARQAIDQRSSLPENAEAVLDPKSLQIIDAVGRAEDGGTGEFIR
                     EAARLIDRARGRMRKDDPDEALRIWKALVEGRWSMVDWFDSDGRRFVLAHPNPPHLRD
                     PRGLTERERQVATYAVLGETNKLISYRLGISQPKVSMALKSAMHKLGVQTRPQLVEKL
                     RGFRSVA"
     gene            complement(18845..19234)
                     /locus_tag="VFG22_14435"
     CDS             complement(18845..19234)
                     /locus_tag="VFG22_14435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417492.1"
                     /translation="MPFAVEISGLVGVLALLVGVWGVLAPRRIAELVSRFGSAGGLWF
                     AAAIRLVFGLGLWFAAPASRAPLLLQVLGVIALIAALVLPFLGVDRFKSLLDWWTKLS
                     PTAMRLNCLVAVAFGAAVLWALLPAAS"
     gene            complement(19236..19658)
                     /locus_tag="VFG22_14440"
     CDS             complement(19236..19658)
                     /locus_tag="VFG22_14440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417493.1"
                     /translation="MPTWIDIKDHARSSYKLAEEQEHGFKVVFEYPGGRLQAVIVSHY
                     QTMGRSWVDFSSACCRADRLDPEAALHQSFNLAVGSLCQDGDVYVVRHTVMVDHMRVE
                     DIELSMHAVARAADQIEKSLPIYEPGSDILRDVEEERV"
     gene            19733..21520
                     /locus_tag="VFG22_14445"
     CDS             19733..21520
                     /locus_tag="VFG22_14445"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET6417494.1"
                     /translation="MFERLTSRPWRSSIAAELRFGPKALRGTVPWLLRTRLGQIDSLL
                     HVALKNAREAPHDLAYEMEHEHLTWSELASASSRVAHVLAAAGVKPGDVVAFLAENSP
                     FYIASVLGISRVGATAALLNTNLRGRPLSHAVEVSGAKVVLVSDSLEPGLRECTDTCR
                     SLNRILVFDDNPYAGLLANAPSTSYPPAPVRAEDDFIYIYTSGTTGLPKPCRVSHARA
                     ILAGAGFGPLMYDFRPGDKLYCVLPLYHANGLLLGAAATMLARVPMAMRRSFSASAFW
                     DDVHRYKATTILYIGELCRYLVNSPPHPRELPNPVRVAVGNGLRPDIWPRFKARFGIE
                     HIREFYGATEAPGFIANISGREGSVGRVPLGGLVGWLRIVRYDVDVDKHLRDERGFCI
                     PCADDEPGELLIRVPKISTGGLEYRGYTDESATEKKLLRNVFKKGDQYFRSGDLLRRD
                     ADGFYYFVDRIGDTFRWKGENVSTAEVADVITHDESIDEATVIGIPVPNMDGQAGLAA
                     VVPAGKFSPDRFFRVVSDLPPYAQPRFVRVMSSLAKTGTFKIQKNDLRKDGVDPSSID
                     DPIYVRTANGYELLTEDRWVDVKEGRLKL"
     gene            21556..21960
                     /locus_tag="VFG22_14450"
     CDS             21556..21960
                     /locus_tag="VFG22_14450"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET6417495.1"
                     /translation="MKNSFPQARMPVEIEELRDGYLRIRVPVSDRYLRPGGTVSGPTL
                     MTIADTAMYYLVLGMIGPVALALTTNLNMSFLRAPGLADVIAETKMLKLGKRLAVGQV
                     TIYSDGSEDPVAHATVTYSIPPDAKRPSTPSR"
     gene            complement(21932..22516)
                     /locus_tag="VFG22_14455"
     CDS             complement(21932..22516)
                     /locus_tag="VFG22_14455"
                     /inference="COORDINATES: protein motif:HMM:NF025244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4202 domain-containing protein"
                     /protein_id="HET6417496.1"
                     /translation="MPALDEALRRFDAANADDPNTELVDGQPLPKELVYGQRMSARLS
                     VFAPEASDAVQLAARAQHIRRWEVPRSSYPEGRAGYLKWRTDLYKRHGEIAGAIMKDV
                     GYDDALVMRVQTLLRKRGLKTDPEVQLLEDVICLVFLEHYFHDFAQKHSEEKLLPIVQ
                     KTWKKMSEQAHDAALQFEYAPEDRSIIEKALKGA"
     gene            complement(22565..23143)
                     /locus_tag="VFG22_14460"
     CDS             complement(22565..23143)
                     /locus_tag="VFG22_14460"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HET6417497.1"
                     /translation="MTNGILEADQEMLGTIKRHAGVGTAVGILVVVAGILALLSPLVA
                     GLAIAIAVGVLLIASGVSRLFLAFRMGSFGHGLLVFVLGALAIIIGGYMISRPGMALG
                     TLTLVLAVYFAVDGVFQILWAFRLRPIKGWGWALFSGIVSLALGVMIWRQFPVSGVWA
                     VGTLAGIQMVFGGSSVASVCAAARGAAKDASP"
     gene            23282..23890
                     /locus_tag="VFG22_14465"
     CDS             23282..23890
                     /locus_tag="VFG22_14465"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6417498.1"
                     /translation="MDVRAQILTHATRLFAEQGYDGTSVQQIADAVGIRKPSVLYHFK
                     SKDELREEVLSEMLAHWNTVLPGLLLKASAEERFDATMEALTEFFIVDPERARLFLRE
                     TLDRPEQMQSMLGAFVQPWVELLGDQLERAKAQCLVRRDVDPHAYAVEVITMAVAGTA
                     VIDTLRVILPDDPVRGSTRIRHARELIRIARSSLYTENEGEK"
     gene            23893..25821
                     /locus_tag="VFG22_14470"
     CDS             23893..25821
                     /locus_tag="VFG22_14470"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6417499.1"
                     /translation="MANFFKDNDDLQYYFDKGIDWDTVVRITEHEFADAEGDGFKDTQ
                     EAVEFYREIVEMFGEFVAEEIKPHEKEIDEEGVALVNGEVVFPTRLAGIFEKINELDL
                     HGLPLPRELGGMNAPMLVYFMNSELMARADVSTMAHHGFHAGIAMAALVFSAIEGSSK
                     IDPKTGRIDETRWRHVIEEIAAGKAWGCMDITEPDAGSDMAALRAVGEQDADGNWHVT
                     GEKIFITSGHGKYHFVIARTEKSDPDDPMDGLNGLSMFLVPAYEEDAEGNRRRIVQLS
                     RVEEKLGHHGSATCSLVFDKAPADLVGERGEGFKYMLTLMNNARLGVGFECIGLSESA
                     YRVATQYASERASMGKTIDRHEMIAEYLETMRTEIQGVRAMAVTACFHEEMAQKLSLA
                     VRFRDLDASTRQQMERLMRSHQKKARRITPLLKYFAAEKAVEHARTALQIHGGNGYTT
                     EYLPEKLLRDALVMPIYEGTSQIQALMAMKDTLGAIIKSPQAFVRRLAQARWRSISSR
                     NPLERRVAQLQSLSLSAQQHLVLKTAGAKIRGLRGKPIGDWSDDLTKNWNPKRDFAFA
                     MLHAERLIQILGDTTIAELLLAQAAKHPERAEVLERHLERAEPRARYLLDQITTTGQR
                     LLEKLSPESAEAMGQAAE"
     gene            complement(25856..26851)
                     /locus_tag="VFG22_14475"
     CDS             complement(25856..26851)
                     /locus_tag="VFG22_14475"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6417500.1"
                     /translation="MAGPKPDGKVLITGASGFIGSRLRAALIAAGSDVVAIRRPGSPP
                     SKGGRSVQADYARLEDLERIVGDEKPDYVLHVAGVTKGVSYQDFQTGNVMPTRNLLTA
                     LGRAHVDVKRFVLVSSLTSYGPSATSAPHREADPPRPIEYYGQSKLEAERVVEEESAG
                     IPWTILRPSGVYGPGDVDYFNLFKSAMRGWNVFFGNRDRLMSVIYVDDCVRGILEGAQ
                     HPSSEAKGYFLTSEQQVSWEQFQSEILRVVDRRVRTINLPSQLVTVAALGGELATRVD
                     KKPRLLNLQKAKMGAQQAWTCVGDAARRDFGFEAQIGLSEGIRLTHEWYQANDWY"
     gene            26930..28324
                     /locus_tag="VFG22_14480"
     CDS             26930..28324
                     /locus_tag="VFG22_14480"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET6417501.1"
                     /translation="MTGSFKTISPVDGSVVFERSLDGPKEIEGTLAAASKAFESWHQV
                     ALEERCTIVRRFVELAVHDTDAVAQELTMQMGRPIRDGGGEAGGWLLRGRTMIDLAPE
                     ALADIRLEARDGFTRFIRREALGVVFVVAPWNYPWLTAVNALVPALVAGNSVVLKHSE
                     QTPLVAERINRASREAGLPKGLLQVLYLDHAGVADVIADPRIAFVAFTGSVEGGHAIQ
                     RAASKRFIGAALELGGKDPAYVCADADVARSAANLVDGSFYNAGQSCCGIERIYVHRE
                     VYDTFLEAFVSVANELVLGDPRDSLTTLGPVVRVRNAESIQAQIDEALTAGARPLLDP
                     KHFQASKLGLPYMAPQALVDVDHSMRIMTEETFGPAVGIMRVEDDAEAIAMMNDSAYG
                     LTASVWTSDLDHAQEIGSRVDTGTWFMNRCDYLDPELAWVGVKDSGRGCSLSTLGYAQ
                     LTRPKSFHLRHAPG"
     gene            28363..29106
                     /locus_tag="VFG22_14485"
     CDS             28363..29106
                     /locus_tag="VFG22_14485"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6417502.1"
                     /translation="MKPPVLRCRHVTKRYGKFEALKDLSLEVYEGEIFALLGPNGAGK
                     SSLIHCITGLSKQTDGSIEVLGFDTLNDFRTTRRLVGLVPQEINYDPFFTPLESLMIQ
                     MGLMGVEPSRARALSLLETFSLDEKRDAYTRTLSGGMKRRLLVAKALVHEPRLLFLDE
                     PTAGVDVELRRELWDEVEALRMAGTTIVLTTHYIEEAEKLADRIGILHKGELLLVESR
                     DSLMERHGGRSLEDIYLELIQKADGRHAA"
     gene            29103..29864
                     /locus_tag="VFG22_14490"
     CDS             29103..29864
                     /locus_tag="VFG22_14490"
                     /inference="COORDINATES: protein motif:HMM:NF011648.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6417503.1"
                     /translation="MSTIALKTLFLKEIRRFSKVWLQTVLSPLVTTSLYFLVFGVALG
                     SRLREISGVPYIQFVVPGLVMMTMVRDSFLNTSSSLFQSKINGTIIDILVAPIGALEM
                     LLAYVGAAMLRAMIVGFLVYLVALTFTWFPLHHLGWILFFTIFVTATLALLGMVAALW
                     AQKFDHLAIFPNFVLLPLAFLGGVFYSIDMLPEPWNTISRLNPLLYMVNGLRYGFLGV
                     ADVSPGASAAIVVISFFAVLGVASALLRSGYNLRT"
     gene            29884..31482
                     /locus_tag="VFG22_14495"
     CDS             29884..31482
                     /locus_tag="VFG22_14495"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6417504.1"
                     /translation="MSSSTYFADPAARAFLQRRVAAFGLIGALLGGTALLFRMIMGIL
                     FGFIRQQITDPGFLIHAAAILPLLGIWLICRRGKLSVNAIHAVENTGIFLASVCYIGM
                     GMDIRAEVGADTITAFVLALMMFARSVYVPSSAKRTTILGILIGVPLVAAMYYHYLGV
                     DLRIWRSFGYEGQSVQRVATSQAIITTMWWTLTTGLAALASRVIHRLRKEVSDIQSLG
                     QYKLERKLGEGAMGVVYEATHGMLKRPTAIKLLRPELADPDALERFRREVQLTAKLTH
                     PNTVTIYDYGRTPEGLFYYAMELLSGATLTQVVTASGPQPVERVVRILRDAALALNEA
                     HDVGLIHRDVKPSNIMLARQGGVPDVTKVLDFGLVKNLGKVDDLEQTNTMSIKGTPHY
                     LSPEAIQDPQGIDARTDLYALGAVGYYLLAGRHVFDGKTIMEVCLHHLQTKPVSLAEV
                     CQTEVPAGLEELIFACLEKRQADRPATAQELADALDRLKVARWTRADAEAWWDVFGQE
                     IEKAKQPLSSSGTEQTLAMDLDER"
     gene            31545..33833
                     /locus_tag="VFG22_14500"
     CDS             31545..33833
                     /locus_tag="VFG22_14500"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HET6417505.1"
                     /translation="MKASVSALALSIAVLLGSCSSDSQVGVAQNIFADLGAPLPSATA
                     AELRSFERGRTAALRRFTPSDGLGPEFNLTFCAGCHEKPVFGGGASHYRDFLLVGDEL
                     YPDTVVPRGKNGVQRQFSLETGRAPSDPLTNISATRNPIPFFGAGLLAEIPDTEIVSH
                     ADPDDSDGDGISGRVNYDGVLVGRFGRKAQTSRLELFVRGPLFNHLGITSEPLSEARR
                     AELRSFSASAGVVTAIPKQAVIPDEPTVDLDDVPDPELSEDELFDLVAFASLLAAPRP
                     DESTDETERGAITFSEVGCDRCHLPSLSGPRGSIPAYTDLLLHDMGDELADRFPMGQA
                     TGREFRTQPLWGIAAASPYLHDGRAATLDEAIQWHGGEATEIRDAYAALPAGGRADLI
                     AFLESLGGRAQSSPGLMPPDAPVPSQGEYGAPVVGLSAEEQALFEQGRLLFDRDFSFS
                     EGVGPSFNGDACRSCHFDPVIGGAGPSGVDAMRQGRVEAFIFSAPDGGTAIPRHAVNG
                     VRPEADPSSNFFERRQTPSTLGLGLLEQVPRETIEALADPSDENDDLISGRVHILDDG
                     RMGRFGWKANVPSVREFVRDAFSGELGVTVPEEPGFTFGLAADDDGVADPEVDVSSID
                     AITAFIQFLDAPPRDRSDETLEDRGEAIFDVVGCAQCHVPNLETRDGTELHAYTDLLL
                     HEIAKASDLGIEEGDAGIHEFRTPPLWGLSRTAPYLHDGRASTIEDAIAEHSGEALTV
                     RGAFEALSPADQDALLAFLRSL"
BASE COUNT         6765 a        10478 c        10450 g         6293 t
ORIGIN      
        1 cagagtcgca aagttcagta cctgacgcgt cgaatcgagg acgcggtcac cgcttggacc
       61 ggagccatcc tgaacgggct gcttcgtgac gccgaaaagg aagccctgtc aatcgacctc
      121 tacgatgcct cgtttcgacc cgacaacgat catcaggggc tctatgaatg ggagcggcaa
      181 tggttcagac gacgcttgcc cggtcctccg gcgtccatcc tgatcggtgc cgccggtgcg
      241 gggcgtgaag cctgtgccct tcgagagctc ggctatcggg ttcatgcgtt tgagccctct
      301 acatcagctt ttcgggtctg tcagatcaaa gtcggctcgg agtttgtcga ccaagcgagc
      361 tatcaggatc tcgttgcgac ggtactccgt ggcgagagca cgcgactacg actcgcgaac
      421 gaagcaaaat tcgatgccgt gctgctgggc tgggggagct tcggacacgt gcttcagcgc
      481 gccgaccgct tggagttgct ccgtgcctgc gacctcctcg cgccgaatgg cccgattctg
      541 ttgagtatct tcgaaccgaa cgccacagcc tcgacgaatg ggacctacta catgccgtgg
      601 ggtggcttcc tggtacagcc tacacccgaa gagctcgatc agcacgcgga ggccttgaat
      661 cggcaactcg tcatgtcgct tcaggcgccg agcccgtacg ccactttgat ccccaacggc
      721 tactgaaacg cgtcaacacg cggcgcgccg cttcgtacgc gtgcgcaacg agcgaacgcg
      781 aacccgctac cagggagcga tccaagaggt gtggtgtcca ccatcgaggc gagcctgcgt
      841 ttgcgctcgt atctagccgt accatcgcga tcagaatccg gtcccagcgc gagagctcca
      901 atgcgcgatt tgctgcgcat gcgatggggt catcgcgaaa cgactcatgg ccgagcacat
      961 agccagctgc acgctgaagg cccaacgcgc gaaaatggcc tcccaaggtt tctgcgtctt
     1021 cgagtcgata gaggttttcc tttagcagcc atcgaatgtc gtcgaggctc gtgtcctgac
     1081 gaaatgtccc gcgtcccttt acgaaatgac cgatcaaatg ggcgaagagg tcttgatcag
     1141 acatccgtgc gactggcgcc gcaaaaagcg atgcgtctat cgctgcccgc gagaaaacgt
     1201 catccgtaga catgccaaac aggccgtgtg gaaagaggtg gtggtgcacg tcgatgctga
     1261 gcggccacaa atcgctggca aaagtactcc ctgcgcttga atccaccgtc gcttcgaacc
     1321 ccagcgcgtg cagcaccaag gccaccgctt cgatatccga gctgcgtacc aggaggtcga
     1381 tgtcgaccag atcccgccgc ccccgcaagg caggccacct cgccagcaag aacgcgccct
     1441 tcagtggcat caggacgatg ccccgctcgg cacaagcctc gaagacctga tttgctagac
     1501 tgagggccag ttcgccgatg agcgtggtgc gaaggcggtc ttgagcagca tggtccactc
     1561 gggcaatcat cgaccgtcat cgatgcgcgc gtcaagacgg ccatagccga ccccccaagg
     1621 ctagtgggac gccgtcgagg acgacctggc atgcgcctag gccgttcggt ggtatagagc
     1681 gccgatggct acgcatcaaa ggctcggcga tcttgcagag gcgctcgaag cagagggggc
     1741 ggacgagctg cgcgtgcacg tcgtccggcg cgcgcgcgag ttcaagcgct cctgggtgaa
     1801 catggcggag gcgctcgtcg aagtccggaa tcgagagtcc tacctggact ggggctatga
     1861 agacttttat tcgtattgct ccctcgagct tcagctcaag caggcaacgg cagataagct
     1921 caccggctcg tacgtcgcgc tcacgcgcca tgcgccctcg gtactgaagc gcgatggtct
     1981 gaatgagcgc atcccgacct gcgacgcggt cgactatttc gcgaaggctc tccaaagaaa
     2041 tccgagcaac gatgcggacg gcgaacgcgg cgtgcgccag gaagtggtcg atcagctgag
     2101 agaagcggtc ttcgaagaag gcgccccggt ggccgagctc cgaaagcggt tcaacccggt
     2161 tttcagtccc aagcccgagg gtgcggagta tctggacgga atccggcgcg cgacggcgac
     2221 cgctcgacgc ctcgagaaaa tgctcgaaga gatcgacggg ctgccgcggc cgacggtacg
     2281 cgccgcgctc gacagcctgg aggcgctccg agaagacctc acgcagctcc tcgagcgaac
     2341 caaggcccag tacgcgaagt cggcttgaac atgagtgaaa cgtgttctag tccatgggac
     2401 cctgcgccat gactgagaaa acgctagcgc cggccgtaga aggcctcttc accatggacc
     2461 cgcgcgagcc gcatttgctc ggaacgcgat gccgcgcctg tgggacctat ttcttccccg
     2521 ccgagagaac cttctgtcga aacccggctt gtgacgaggc tgacctcgaa gaggtgccgt
     2581 tgagtcggac cggcaaggtc tggtcgtaca ccagcgcgag ctacaagcct cccgctccct
     2641 tcgtcgcgaa ggagcccttc gagccgttcg cgatcgccgc cgtcgagctc gaggccgagg
     2701 gcatcacgat cctcggtcag gtcgcggacg gcatcggtgt cgatgcgttg aagaccggca
     2761 tgccgatgga gctggtgctc cgcgagctct accaagacga ggagcacgtg tacctcacct
     2821 ggaactggaa gccgggcgac gacgggggcg cagcatgagc aaagacgtcg cggttctagg
     2881 cgttggtatg cacccctggg gcaagtgggg tcagagcttc gtcggctacg gagtcaaagc
     2941 cgcgcaggat gcgttgaaag atgcgggcgt gcagtggaga gacatccagc tggtcgctgg
     3001 aggcgacacg attcgaaatg gttacccagg attcgtcgcg ggcgcgacct ttgcgcaggc
     3061 actcggctgg tcgggggcaa aggtgtcgag ctcgtacgga gcctgcgcaa ccggagctca
     3121 agcgatcggc gtcgcgcgcg cacacatcct cgcgggcctc tcggaagtgg cgctggtgat
     3181 cggcgccgac tcagctccca aaggcttctt cgcccccacc ggcggccgtc ggcccgagga
     3241 cccggattgg ctccggttcc acatccaggg gatcaccaac ccgacctatt tcggcttgta
     3301 cgcgcgccgg cgcatggatc tgtacggcgc aacccagcag gacttcgctc aggtgaaggt
     3361 aaagaactcg aagcacggcc ttcacaatcc gaacgcgcga taccgcaaag aggtggcact
     3421 cgaagacgtg atgggctcgg tgctggtcgc cgacccgctt caccttctcg acatctgcgc
     3481 aaccagcgat ggcgccgcgg cgctcgttct ctccagcatg gacttcgcac ggaagcggac
     3541 gacgaatccg gtcaagatcg ccgcggtgtc gacggtgaca ccggtttatc cgaacgacgt
     3601 catcgagatg ccgaacctcg cgaccgactc gaaagccggc gttggcctgc cggagcttgg
     3661 gttcaaggaa tcgatcgcac accgagcgta cgaagaagcc ggactcggcc cggaggacat
     3721 ggatctcgcc gaagtctacg acttgtcgac ggcgctcgag ctcgactggt acgaacagat
     3781 cggcctctgc ccgccaggcg acgccgagaa gatcctgcgg gacggggaca ccaggatcgg
     3841 cggccgcgtg cccgtgaacc cgagtggcgg cctctcttgc tttggtgaag cggtgccggc
     3901 ccaggcgatc gcacaggtct gcgagctgac ctggcagctc cgcggccagg ccgaaggacg
     3961 ccaggtcgag ggtgcacgag ccggtgtgac ggtgaaccag ggcttgttcg gccacggctc
     4021 gtcggtgatt tgcaccatct aggtcaatcg aggcgcgcga gctcttcgtc tagccgagca
     4081 tcgaggttgg actcccatgg cctcgcctcc gctccgcgtt ggctcgagcc ctgaccccgg
     4141 aacttcagag ccatgatgat gagccagagc gtcgaaagca ccacggcgac gagcgggatc
     4201 acccagccag atttcacagg cggaatcctg aggtcgaact cgggcacgcg ttcctgcagc
     4261 cgggttcgaa tttcaggctc cgggacgcct tctgcgaccc aggagcggat gtcgcgccgc
     4321 cactcccccg ccttcggact cgggcaagag tcgagggtct tgcctgggca gaacgggctg
     4381 agagtgttgc gaacgatggt ctgaacttgt cggctctcgg gaggagcctc gtcgacgggc
     4441 accggcgcgc agcccaaggc gagcaagagg aaaagcgcgg acgcggtacg caacatcaag
     4501 cgactacata atcacccaca gtccgagctg taaggtggca aattgtcgcg gtaccggctc
     4561 tgcactaggc ctccgctaaa ggagcatacc ggcgacggcc gccgtcatca gggacgccag
     4621 cagtcccccg aacatcgctc gcagccccaa gcgtgcgatg tcgtggcggc gctcaggggc
     4681 gattccgccg atcccaccaa gctgaatccc gatggagccg aaatttgcaa atccgcacaa
     4741 agcgtagctc gtgatgacga cggtgcgcgg agatagcgcc gctacaccct cggagaggga
     4801 ctcatagagg tgcaggtacg caatgaactc cgtgaggacg agcttttcgc cgagcaaggt
     4861 tccgacgctg gcgcactcgg cggcgggcac cccgatgagg aatgcgaacg gccagaacag
     4921 ccaacccaag atccgctgaa gcgtgagagg ctcgatcgcc gcggagcctt cgagccccat
     4981 ccaatcgttg tagagcccaa atggcaggcc gatcaacgca ttcgcgagcg cgatgagggc
     5041 gatgaacgcc aacaacatgg ctccgacgtt gagagccagc ttcatgccct cataggcgcc
     5101 tcgcgccgcc gcgtcgatga cgttggcgtc cggttgatcg atctcgacgc ccaacgatcc
     5161 ggcggtctcg gatacttcgg tctcgggaca catgaccttt gcaatgacga gggcagccgg
     5221 cgcactcatg acgctggccg cgatgagatg ccccgcgatg tccgggaaca cgcccttgag
     5281 gagtccaaca tacgcagcga ggactccgcc cgccacggtc gcaaagcctc cagtcatcac
     5341 ggccatgagc tccgagatcg tcatggtgcg aacgaacggc gcgaccatca gaggcgcttc
     5401 ggtctgaccg acaaatgtgt tggcggcagc ggacaaagtc tccgagcccg aagtgcccat
     5461 cgtgcggcgc atgacccaag cgaagagccc gaccaagcgc tgcatgatgc cgaggtagta
     5521 gagaatcgtc atcaacgaag aaaagaagat gagcgttggc aagacgttga tcgcgatggt
     5581 gaacttctcg gacgcaaagt caccgaagac gaactcggtc cctttcgccg tgaacccgag
     5641 cagggcgagc atcatgtcgt tcaagatcgc gaacacgcgc agcccgaggt cggtcttcat
     5701 cacgaagacg ccaaacgata cctgcaaccc gatcccccag ctgacgaccc gccatggcac
     5761 gcgatcgcga tgcctggaga ggagccaggc aatgcccacc atcgcgagga gcccagccag
     5821 cgaaaccagg cgctgccaaa aaggaaccga tcccaggtcg ctcatcatcg accgcatgct
     5881 aacgccttgc cgaggcgcca gagggctttt ttgcctactc gtagggaact ttgaaccggg
     5941 cgcgataggg ggcgattcgg gactcgacgt cgtccctggt cattccgaag tcggccaggg
     6001 agtagctgtg gcgaccgtac ttgtgctgct tgttgatctt ccgagaggcg ttcatggccg
     6061 cgagggcctc tgagctcagc tcgtggccgg cggcttcgta gacacgggcg acctcgggaa
     6121 tcgggtctac gaccagatcg tagtacgaca cgtctacgaa accctcgtcg ttcacgcgct
     6181 cgcgggtcgc catggtacgt cgcgccatgt tttcgatctt gtagagccag tgttccgcaa
     6241 cccggtttgg atcgacctcg tcgctgaaga tcatttgcag gtgcgtgagc atgctgaaaa
     6301 gcgacgggct cgtcttgagc gggtcgcggt gcgtgtggac gatcgtcgcg ttgggaaaca
     6361 cctcgagaag tggatcgaaa tactcctgat ggtgcggggt cttgagcacc catcgcaggt
     6421 cgtggccgat ccctggtcgc tgccactgca gatactgcat catgcgcttc agggcttgat
     6481 acgcaggaac gtgatcctgc tcctccagcc atttggaata gctgggcacg ttcatcatgg
     6541 cctcgggcgt cgtcgtgaga aacgcctgct cgaggagaat gacctcttct tccggtgcat
     6601 cgggctccgc cgggtgtatg gcgaaaaacc cgggagacat gtacttgagc cccttcgcgg
     6661 ccgcggcggc ttgagcgaaa cgcggatccc tcttcccgag gcgctttggt ttcgccggtg
     6721 ccggattgat cgcctcccag gaggcgagcg aacgaatccc cggatcctcc gcgatcagcc
     6781 tgtgcagcat ggtcgttccg gtgcgcgcga gcccggccac gacgatgggt gcttcgaccg
     6841 gaatctccag gatttccggg tgccgttcga tcgtgttttg cgcgacgagc ttgttgacca
     6901 agacgcctac gatccggccg cgaatgatca tgtggccgac ggggttgaga gcggcctcgc
     6961 gattgatcga atccacgaga atttcgagcg gctcgagaaa gccctcgtcg ccgaagtcac
     7021 gaagccccac cttgcgccgc gcatcatcga ggacgccggt cacggtgatc ggcttgccgc
     7081 cgatgcccac acgggaaagc gcgcgcccaa ttcgattggc aagcgcgatt gcagcgggcc
     7141 ggtacgcccg ttcgaagttg ctcgtgatta cgtcggacga cattcgaagg ctcagagctc
     7201 ggagagcttc accactcggg tctgaggctg agggttctcg ttcgcgcgca cccagcgcca
     7261 acacatcgtg ccctcttcat ggccgaccgt gtcgatccaa ttgtcgaagc ccttgttctt
     7321 cgcggcgacc actacctgca ccgacccgtc aggccgatac ttcgccgtgt gcttgttcac
     7381 gtgaatcggg tagtagcgat agtcgagcga ttccatccag tagttgttga gctgaaagtt
     7441 ccaatgatcg cagtcaggag gcattgcgtc gatcaccagc gcttcatccg gcgcaagctt
     7501 ccaatagctg tggtagtaag cgatgtcagg cacgccacca gcttggttgg agacctcctg
     7561 cggcatccgc gggagctcgt tcgtgtgctt cttgaagttc tcggcccagc tcgcaaacat
     7621 gatggcggcg ccgaccacga ggcccgcgga ggtcgtgagc ccctcggcac acttcacggg
     7681 gtcgaatggc gacggctgcc cgtccccacg aattcgttcg attcggagct cggcaagctt
     7741 ctcggtctcg cggtccaaga acgtctgtcg cacgatcaac gtgccggttt ctctagtcat
     7801 cggaagccag tttcctggct ttttctccga gctgagaatg atctcgaagc tgccatcagg
     7861 ctcgatcttc atcgtcgacg cctcgagata ccccgtgggc ggcatgcccc gaccctgccc
     7921 gtagttaccg gtctgcgtga agaaaccgag atagtgcact gaacctcggt tgccgactat
     7981 ccggtactcg tgctcgccgc tgatcgcagc gttctgataa aagttgtcgg gatggtcggc
     8041 gcccatcttg gccgtctcgt gaaccaccct ctgcaagacc ggagccaatg ggtcgttgtg
     8101 ctcgacaaat gtctgcaaag ctgcgcgcgt gattcggctg agatagcgaa agccttccat
     8161 gcggttcatc ggatcgctcg gcgtgttcgg accaagcacg acgttgcccg ccatcttgag
     8221 tgtgtcgcag aagcgattcc aaaccgttcc gtctaggatc tctcgttctt ccttgctcag
     8281 ctcgtcggtc attcggcctt cctaaatcac accatacctg cgcaagagat cttccgtctt
     8341 actccagagg agcttcccgt tcgcgggatt catcgaggcc gcggaagact ccttgatgcg
     8401 catcatgtgc atgtattcgc cggtcttgtg ccccatctcc tcggagaccg cgagataatc
     8461 tgccgggatc gaagccttct cggggctctg aaaaaacaga gccatgaccg gcttcaggat
     8521 gggcttgagc caggcgggcg cttcacgcgc catgttggag ttcaccgcgc cgggacaaag
     8581 cgcatgcacg cacacgtccg ggtcctcgcc attgctgccc ggattgagac gccgtgaaag
     8641 ttcgtggaaa taggtcgtga ggtgcaattt gctgagacca tagtacttta ccccatcggc
     8701 cacgccgtac tggaccggcg cgccgaaggc ttcgaaatcg atcgggatgg agcttcgatg
     8761 ggtttcagaa gagacgaaga tgatcctcgg aggatcttcg gcccgggaat tgttcccgta
     8821 gactgcgttc ggaatcacgc catcgtgtaa gaagcgattg agcaaaacga acttggccaa
     8881 gaagttcacc gcgaacatca gctcgagccc gtgcttgttg gttcgcgcgg tcacgggaac
     8941 gactcccgca ttgaacaccg ccacgtcgag cgtgacgttt tcatcacgaa gtcgatcgca
     9001 gaaggcagcg attgaatcga agtcgctgag gtcgacttcc atcatgacga cctgatcgct
     9061 tccgctctcg gcgcggacgt cctctcccgc ttcaggaatc ccgctccggc aggccatgat
     9121 cacgcgcgcg ccgcgcttcg ccagaccgat tgcgatggcc ttccctaagc cgctgttggc
     9181 accggtgacg agacaggtac gcccgtctag ccgcaccgat tccgcaacgg cctcgacctt
     9241 gggctctttg ctgcgctgat cgagcaggcc gctgaggacg gccgcgacgg gattgtcgtg
     9301 ggttctgtta cccatggccg cggaactcta gggatctggg cttctctgtc aacgcgagcc
     9361 cgtaatttgc agatattccg caacgactcg atcgagcccg acgaaaagcg cacgcgccat
     9421 cagcgcatgg ccgatcgaaa cctcatcgat gtcggaaagt gtcaaaaacg taggcaggtt
     9481 cgtgaggtct agatcgtggc ccgcgttgat gccgagacct cgtgcagtcg cgcgctcgat
     9541 agcccgtcga tatcgctcga ggctcgcgac gaggacctcg cctccctgct cgaacgcccg
     9601 cgcatagggc tccgtgtaaa gctcgacgcg gtcggctcca agctcggcgg cccaatcgat
     9661 cggcgcaggc tccggatcga cgaacacgct cacgcgcaca ccatgccgct gaaagcgttc
     9721 aacgatctcg atgagctcgg actttggcgt ctcggcaggc catccagcct cactggtaag
     9781 ctcacctggg cgaaccggca cgagcgtgca ttgatgtggc cggacttgct ccaccaactc
     9841 gaccagcgcg ggccggggat ccccctcgat gttgtattcg acccgatccc gcatgggctc
     9901 gagttgctcg gcgatttcgt acacgtcgga tggccgaatg tgccgctcgt ccgcgcgggg
     9961 atgcacggtg atccccggcg cgccggcgtc cacgcaggcc cggacggctt ccagcaagct
    10021 cggtaccgcg ccgccccggg aattccggag cgtggccacc ttgttgacgt tgacgctcag
    10081 ccgcaccatc gcggccattc ttagctcccg aagggacgtc tgtcagcatc ggctggcctt
    10141 gcgaagatgc atgcgctaga agaaaatgaa acacgttcta ttacgcctga tggaatgtgc
    10201 cctaaaatcc agtaatattt cgccatgaac gtgagcgcca aagctgtcgt gctggaagtt
    10261 ctttcggcag gcgagtcgat ctacgacggt gggctgcccg tccgttcgct cgtgaaggcc
    10321 gcttcggtgt tcgacatcgc ggagaacagc gtccgcgtcg ccatcgtgcg gctgcgagcc
    10381 gaaggtttgc tcgagtcgcc aggacgcggc gaataccgac tcggccccag tgcgcaagtg
    10441 gtcaaccaga agattcacgg ctggcgaaca gtgtcggctc gcgtcggctc gtgggacggc
    10501 tcgtggacgg gcgtgttcac cgctgatctc tcgcgtacgg atcgccctgc cctgcggcgt
    10561 agacttcggg cgctccgctt ctacggattc gaagagctca aaccggggct ctttctgaga
    10621 ccgaacaatc tcaagcccgg aatcgacggc attcgagagg agctcgttcc gcttggtctc
    10681 gacgcggatg cgttggtctt ccgcatggac gagctcgatt cggatcagga acgacaggca
    10741 cgggcgatgt ggaatggcgc atccctggag aagaactacg cgaagctcga gggcaacctc
    10801 aagctgagca tggaacgcct cgacaagctg ccagtcgacg actcggtgcg cgaagcgttc
    10861 ctgctgggcc gggagggcat acgtcaggta gtgctcgacc cgctgctccc cgggcccttg
    10921 gtcgacgaaa ccaggcgtgc ttccacggtc gaagttctaa agagctactg tgacaagggc
    10981 ctaaatctct gggctcgctt cctccagatc gattaagggt tcaattcgag tatgctgcca
    11041 ttgatatgaa gtcggcggat tttgcgtttc agacaataga cggtgcgaac cttcacgtga
    11101 gcggctggac ggtggagcag ccgaaggcga tcgtgcagat cctgcatggg atggccgagt
    11161 acgggtcgcg gtatgcgcgg ctcgctgggg cactggcctc cgccggatac agcacctacg
    11221 cgcacgacca tcggggccac ggcaggagca ttccagttgg cagcgagccc ggacacgcgg
    11281 cggacagcga cagttggaat cgtgtcgtgg aggacgccca cggcgtgaat cgcgaaatcg
    11341 cgaagcgcca tcctggaatt ccgatcatca tactcggcca ctcgatgggc tcgttcgtcc
    11401 tacagcaact tttgttcgag cacccgaacg acatggtcgc tgcggcgttg tcgggatcga
    11461 acggaaagcc gcctgccatc gcagtgctcg gcaagctgat cgcgcgcctc gaacgtttgc
    11521 gtgtgggcaa gcgcaaaccg agcccgctgc tccaaaggct cacctttggc gagtacaaca
    11581 aaccttacgc ccccgcgcga acggagttcg aatggctctc gcgcgaccct ggcgaagtcg
    11641 acaagtacgt tgcggatcct ttgatgggat tcgccgtgtc gactcagacg tggctcgact
    11701 tgctgaacgc gctcgaccga atctcgaatc ccagcaacat tgccaaggtc ccgaagggga
    11761 tgccgctcta tctcttcgcc ggcgaccagg atccggtcgg cgacaagggc aagggaatga
    11821 agaatctgta tgacgcgtac aaacgcgccg cgatcttcga cgttcggctc aagctctacc
    11881 cggagggtag gcacgaaatg ctcaatgaga cgaatcgcca ggaagtgatg gacgacttca
    11941 tcgcatggtg cgacgagatc atcggggcgc gttcctaggg atccggtgtg cggctcgtgt
    12001 agagcggccc ctcgacctcc cctcggagca gtgggctcgt ctcgggcgtc attcggcacc
    12061 gccacagcgc gctgcgtgaa ccgaccgcct cggccccgtg gtactcgttg aagtcttcga
    12121 tcaccgcttc gatcagaaag cctgcatcgc cgagcgcacg ctgcttctga acgccacgct
    12181 cgctagcccg acgactctgg ccgaaacaga cgtagagcct accgtcgggc ttgagcagat
    12241 ccacagcccg gctcaggaat agccgaaagc cctcgagcgc atagggcgga tcggtgaata
    12301 ccgcaccgta gcgagcccgc atcgctttgg gaattggttg ccgtaaatcg tgggaaaccg
    12361 tggccagctc ccaggctcgc ttttcggcgt tacgtctcaa gacttcgaga atctcctcgt
    12421 cggaatcgag cgccgtgact ttgcgggctg cgcccgcgag ctgcattgcc gcgctggtca
    12481 aatcgtcatc gcccaggaac acgagcccgc gctggtgctc acccgcctcg agcagaagct
    12541 cagctcggcg aaccgccgta tccgtcgtga cgtaaaactg atcgagcgca cgctttggcg
    12601 tcgcacgctc ggccgcaatt tcttcgtaag ccgtgcgcca ggaagcgtcc cgcggctgac
    12661 ctgaaccccg ggcaaccagc tcgcgcgcca gtgcggccaa gccgcactcc gagcagcgta
    12721 tgcggcgctc gtcgatttca aaccattttg ggtatcgatc cgtgacctcg cgaacgaccg
    12781 acgaagcttc gcctgccgta tggacgagct cgtcgcgaga caacgatcga ccatcgccga
    12841 gtgcccggag gatgcgaaac gctccttcgt acacggcggg atctacttgt cgcgaaacac
    12901 gatcatgccg tgcgtcagat cgtaggggga aagcgcgacc gtcacgcggt cgccgagaat
    12961 cacgcgaatc ctgaacttgc gcatgcgacc acaaagctgt gcgcgtactg ccgcgccgga
    13021 gtcggtcgtc acttcgaatt gcccccccgc gtaaacctga ctcaccacgc cactcagctt
    13081 gatgtggtca tcacgcttgt cttcctgctc gaagttgcgg tcgaacttcc gtttcctgcc
    13141 tcttgggccc tgtctcgcca ctcgttctcc ttcttgcttt cgccgcaacc taagtccact
    13201 gggaccaact cgctagttcc agtggcatcc ttcttgatac ctgcagcgca ttggtttttg
    13261 tagcactccc catggaaaag tcagccagac cggatcatgg tgtcgattcc ggtgctccaa
    13321 agacaccccg acgatggctg ggcactcgcc gatcccatga aggtgggggc gccgatgcgc
    13381 cgggttctgt tgaagctggc agacgcgatc ctgccaccaa accctcgcct cgaaacgacc
    13441 ctcgaggacg tgactcggca cgcgctcatc tgccttcggt acatgccgcg ctcgtccgcg
    13501 ctgctcttcc tgctggcaat gcgcgtcctc aactggtcgc ccctgtggcg cctgcgcggg
    13561 tttcgcccgc tgacgtcgct caccagtgat gcggcccggc gccagctcgt cagcgctagc
    13621 caaagccgat ggctgccggt gcggcttctc atgtatgggc cgctcgggct cttcatgtcc
    13681 tcgtacttcg atcaggatta cgtccatcgg gagcttggct atgcgcccgt tcccttcatc
    13741 accgggcgga tcgagcttcg gcgcgcctgg gtcgagggtc aggagccggg cgccgaccag
    13801 gagattcgtc acctgccggt gcgcgcacca tgaccattct ggcccaccgc gactatcgaa
    13861 agctcgaaga acatcaagcc gagatcgtgg tcatcggtac gggcgcgggt ggcgcggtcg
    13921 tgggagcaga gctcgccgag gcgggtcgcg acgtgctgtt tctcgaagaa ggcggctacc
    13981 atccgacctc gagtttcaat ccctacctca ccgaatcgct cccccggctc taccgggatg
    14041 ccggcggcac catcatcatg ggcaggcctc ccattccttt cgcagaaggc cgctgcgtcg
    14101 gagggagcac tgtcgtcaac ggaggcatgg catggcgtcc gccagagacc gtgctcgccg
    14161 actgggaacg gatcactcgg cagcccgatc tcggtctgaa agcgatggag cctctgctcg
    14221 agcgggtaga agaacgcatc catgcaaaac ctcagcatgc atcgtcgatt ggcgaagaca
    14281 gtcgcttgct cgaggaaggc gctcgacgtc ttggctggga ctacgcgcac aatcgccgag
    14341 cgcaggatcg atgtgtcggc tcgaatcagt gtctcacggg ctgtccgacc ggtgcaaagc
    14401 agtcgacctt gctcagctac atgccgcgcg cgctgtcgac cggggcgcgt tgtcttacgg
    14461 aagtgcgcgc ccagcgactc tggattgaac gtggccgctg cgtgggagtg ttcggcaagg
    14521 cgatcaatcc tcggacgcgg gaagaagacg tcgacgttcg agttcgcgcc aaagccgtgg
    14581 tcgtcgcttg cggcgcaatc caaacaccat ttctcttgct cggtcaccgc gacggtcgtc
    14641 cgagcaagca actcggcaag aatttcctct gccaccccaa cgccaaggtc atcgcaatct
    14701 accccgaccc gatctacgcc tggcaaggcg tcagccaagg tggtcagatt cgggagtttc
    14761 gaaacgaggg catcctcatg gcggagaatg ccgtcccgcc gggaaccact gctgctcaga
    14821 ttccttttct cggtcaggaa gctctcgatt tgatgaagcg gtacgactcg atggtgatga
    14881 cgggtgtgtt ggtcgaggat tctcacagcg gcaccgtcgc acggagattt ggcgggccga
    14941 tggcgagata cagcatcacc cggtacgacc acgcgcgctt tctccggggg gttcagcatc
    15001 tggcgaccct ccacttcgag atgggcgcgg aacgagtgat cctgcctttc agcaactttc
    15061 acatcgccag gcacgtcgac gagctcgccg ggatcgagcg cagtcaagga tcccggagca
    15121 cgatcgagct atttaccgtt cacatgatgg gaacttgtcg catgggcagc cgaccgagcc
    15181 actccgtcgt gaacctcagt ggtgaggtct gggacctgcc gggctgctac gtcgcggacg
    15241 cgagcctctt tccgaccgct gttggtgtga acccgcaggt gacgatcatg gcgcttgcca
    15301 cgcgcgtcgc gcaacggttg caagttccga attgacaagg cgctagcttt tgagcagatc
    15361 gtaatcgacc gatgctcgat cttccacgac tcaagcgcat ccggctcatg aagcgaccga
    15421 tcggccaagt gttctttggc catacggtct tgggtccgaa ctacaggttc ctgcccggga
    15481 tcgggatcga catcgccgga atcgacaaga ttccgaacga gcccgtcatc tacgcgatga
    15541 accacacgga tcgcttcaac tactttccat tcatgtacga gatgtggaag aagaaggagc
    15601 gctacatcac ggtctgggtg aaagggaaat actacgagaa cccaatcgtc ggaaccttca
    15661 tggagctcac cagcaacctg cccacggtgt cccgtggcta tatcatcacc aaggacttcg
    15721 ccctcacggt gggcaggcgt ccaacgagtg cggagtatga aagcctcagg gtgctggtga
    15781 acgaagaggc ggcgccgaac ggggactcga ggacggtcga cctctccgga atcccgcgtg
    15841 agctcttgga aaccaagcgc gatatcttgg gcgtgccatt cgaccccggg agctgctcgt
    15901 acgcggtcgg gataaaccgg gtattcactg caatgatggc tgagttcgtt cgcttgaacg
    15961 agcgctcatt cgagctcggg ctcgacttgc tcgtctttcc gcaaggtacc cgctcgatcc
    16021 ggcttccacg cggacacatt ggaatgatgg aagtcgcgct ccggtacaag aagacgatcg
    16081 tgccggtagg ctgcaatgga tgcgacctcg tgtacaccgg ctccttgccg atcgggaagg
    16141 cggggaagat cgtctaccgt gtgggcgagc cgatccgtta cgaggagctt gccgaattcc
    16201 acatcgacga agacttcgtg ccgttttccg ctgaagcgga gcacaagcac agagacaagt
    16261 ttcagggggc agtagatctg gtcatgaacc gtatcaacga cctgctcgat ccggagtatc
    16321 agttcagcga tgatctggag agcacgggcg tccgtggtac gtcacgtttc atctagcgcg
    16381 tagttgttcc atgggacgaa tcgagctaag cggaataccg tggatacgct ccaggcgttg
    16441 agggacgcga cggaaccgaa gcctgtcgtg gcgatcatcg ggggagccgt cgcagggtca
    16501 gaggccgcag cggcgtgcgc agcgcgcggg gcgatcgccg tcgtgttcga acaaggtgcg
    16561 cgtccctatg gcaagatcga agacggccta ccccgctggc acgagaagct gcgcgacaag
    16621 gaatacaaga agatcgacgc gaacctcggc agcccgggag tcattttcgt accgaagacc
    16681 gaaatcggcg gagacctgaa atggtccgag ctccgagacg agcttggatt tagcgccatc
    16741 gtgctggcca acggcgcctg gtccgaccgg ggcttacccg ttgaaggcgc caatcactac
    16801 gaacatcgag gcctcgtctt tcagaacgag ctcgtgtact ggttcaacca ctacgaggaa
    16861 cagggctacc aggggccggt cttcgagatc cccgatggca cgatcgtcat aggcggcggg
    16921 cttgcttcga tcgatgtcgt caagatcgtc aatctcgagc tctacgcacg cgcgctgcgg
    16981 gcacgaggaa tcgaagtcga catcgtcgag atggagatca aaggcatcga gcgaacactc
    17041 gaagcgcacg gcctgaccgc ggaagagctc ggggtgaagg ggtgtacgct ctactaccgt
    17101 cggcggaaag aagacatgcc gctggcgagt gcggacaacc ctaccccgga gcagctcgcc
    17161 aagctccaag cggcacgcgt gaagatcatg gaccgcgtca tccgtaagta cttcgttaag
    17221 ttcgaagagc tgtccgtgcc cgttggcatg ctcctcgagg acgaacgcct ggtggggatt
    17281 cgcttccaga agaccgagct ggtgggaggt cgcgttcgcg ccttggaggg gagcgaattc
    17341 gacgtgcgcg cacccatgat catcagctcg atcgggagca tccccaggcc gatcgacggt
    17401 gttccgacca aaggggagct ctaccactac gacagctggg agaccggcga ggttcacggg
    17461 ctccctgggg tattcggcct cggaaacgtc ttgacgggca agggcaacat caaggacagc
    17521 cgggcgaacg cgatcgagat catcgagaaa gtcgtggcgg cttaccttgg tgtcggcgat
    17581 tctccggagg aagcgccgga tttgccttcg gcagacgctg aagcgcttgc cgagcgcgct
    17641 gtctccggtg tgccaatgag cgccgaggcg atgaggaggc tggcagccaa gatcgaagaa
    17701 cgacacgaag cgatcggcta cgaggactac agcggctgga tccaggcgag cgcttgacga
    17761 gcgtagcgac ctgagcttcg atcacgaagc tcactcgctg gagctcccat gctcaagctc
    17821 agccctgcag tcatcgattt gacggagttc gtctacgatc tggacgtacc gcgggaggac
    17881 tgggtgcctg cgttgctcga gacggggtta ccgctgttcg atcacggtca cggagtcttg
    17941 gcgacgacgt acattcgccc actcgaggga ggactacccg ttcccacgaa gttctaccaa
    18001 cgaaccggcc cttcggacct cgccgaacgg atcttcgtgg cggcctacga agcggacgaa
    18061 gcgactttga gggacatcaa tcgttcgggt gtcgtgacga cgctttcgga ggaacagcat
    18121 cgcattccgg gcggattgaa gactttcgaa cgctatctcg cgccagctaa agatctgctc
    18181 gcgatcacgg cggtcgaccc aaatggcatg gggctcagtc tctcgattct gctgccagag
    18241 gtgacccaac tcaccggcag agatcgcgag atatggaaga tggtcggcgc gcatctagtg
    18301 tcggggttcc gattgcgcca gagcattgaa gaggcgcgtc aagcaatcga ccaacgatct
    18361 tcgcttccgg agaacgccga ggcggtgctc gacccgaaga gcctccagat catcgacgcg
    18421 gtgggccgag ccgaagacgg aggcacggga gaattcattc gggaggctgc ccgtttgatc
    18481 gaccgagccc gcggacgaat gcgcaaggac gatccagacg aggccctacg aatctggaaa
    18541 gctctggtcg agggcaggtg gtccatggtc gactggttcg acagcgacgg ccgccgtttc
    18601 gtattggcgc accccaaccc accccatctg agagaccctc gcggcctaac cgaacgtgaa
    18661 cgacaggtcg ccacctacgc cgtgctcggc gaaaccaaca agttgatcag ctatcgcctg
    18721 ggtatctcgc agcccaaggt ttcgatggcc ctgaagtctg caatgcacaa attgggtgtc
    18781 cagacacgtc cccaactcgt cgagaaactt cgtggattcc gaagcgtcgc ctgagggggt
    18841 gcggctagct cgccgccggc aggagtgccc agagcaccgc ggcgccgaag gcaactgcaa
    18901 cgaggcagtt gaggcgcatg gcagtcggcg agagcttggt ccaccagtcg agcaacgatt
    18961 tgaaacggtc gacgccgagg aaaggaagca ccagcgctgc gatgagcgcg atcacgccaa
    19021 gcacctgaag cagcagtggt gcgcgggagg caggagcggc aaaccaaagc ccaagtccaa
    19081 agactagacg gatggccgcg gcaaaccaaa gaccgccggc cgagccgaaa cgagacacca
    19141 gctcggcgat gcgccgagga gcgaggacgc cccagacccc gacgagaagc gcaagcactc
    19201 cgacgaggcc cgagatttcg acggcaaacg gcaatttaga cgcgttcctc ttccacgtcg
    19261 cggaggatat cagacccagg ctcgtaaatg gggagcgact tctcgatttg atcggcggcg
    19321 cgcgcgaccg cgtgcatcga cagctcgatg tcttcgaccc gcatgtggtc gaccatgacg
    19381 gtgtggcgaa ccacgtatac gtcgccgtcc tgacagagcg agccaaccgc caagttgaag
    19441 ctttggtgga gcgcagcctc cggatcgagg cgatcagcgc gacagcaggc cgacgagaag
    19501 tcgacccagg accgccccat ggtttggtag tgcgacacga tcaccgcctg cagccgtccg
    19561 cccggatact cgaagaccac tttgaagccg tgctcctgct cctcggccag cttgtagctg
    19621 gacctcgcgt gatccttgat gtcgatccac gttggcacgg gccgatcctt gtatcatatc
    19681 gactcgaaaa aagtggctcg aatctgcgac ggcaacgcgt acacttaccg acatgttcga
    19741 gcgcctcacc tcgcgtccgt ggcggtcttc gatcgccgcg gagctcagat tcgggccgaa
    19801 agcattgcga ggaacggtcc cctggcttct ccgaacgcga ctcgggcaaa tcgatagcct
    19861 tctgcacgtc gcactcaaga acgctcggga agctccacac gatctggcct acgagatgga
    19921 gcatgagcac ctcacctggt cggagctggc cagcgcgagc tcacgggtgg cgcacgtgct
    19981 tgccgcagca ggcgtgaagc caggagacgt cgtagcgttc ctggccgaga actccccttt
    20041 ctacatcgcc tcggtgctag gcatctctcg tgtaggtgcg accgccgcct tgctcaacac
    20101 gaatctgcgg ggccgccccc tctcccacgc cgtcgaggtt tcgggcgcaa aggtcgttct
    20161 ggtcagtgac agcctcgaac caggtctccg cgaatgcaca gacacctgtc ggtctctgaa
    20221 tcgcattctc gtcttcgacg acaacccgta cgcgggcctc cttgccaacg ctccctcgac
    20281 ttcgtacccg cctgcacccg ttcgagcaga ggatgatttc atatacatct acacctccgg
    20341 aacgactggg ctgccgaagc cgtgtcgcgt ctcacacgcg cgcgcgatcc tcgcaggcgc
    20401 aggctttgga ccgctgatgt acgacttccg gccgggagac aagctctact gtgtcttgcc
    20461 gctctatcat gccaacggcc tcttgctcgg cgctgccgcc accatgcttg ctcgcgtgcc
    20521 gatggcgatg cggcgttcct tcagcgcgag cgcattctgg gacgacgttc atcgctacaa
    20581 ggcaaccacg attctctaca tcggcgagct ctgccgttac ctggtcaact cgcctcccca
    20641 tccgagagag ctacccaatc cagttcgcgt tgccgtcggg aatgggctcc gccccgacat
    20701 ctggccgagg ttcaaggctc gctttggcat cgagcacatt cgcgagttct acggtgcgac
    20761 cgaagcgccc ggattcatcg cgaacatcag tggtcgcgag ggctccgtcg gccgcgttcc
    20821 actcggaggg ttggtggggt ggctgcggat cgtcaggtac gacgtcgacg tggataagca
    20881 ccttcgagac gagcgggggt tttgcatacc ctgcgcggac gatgagcccg gagagttgct
    20941 catacgtgtg cccaagatct ccacgggagg gctcgagtac cgcggctaca cggacgagtc
    21001 cgctaccgag aaaaaactcc tccgcaacgt gttcaaaaag ggcgaccagt actttcgctc
    21061 cggcgacctg ctgcgcaggg acgccgacgg cttctactac ttcgtggatc gaatcggcga
    21121 cacttttcgc tggaaaggtg agaacgtgtc gactgccgaa gtcgcagatg tgatcaccca
    21181 cgacgagagc atcgatgaag cgacagtcat cggcatcccc gttccaaaca tggacggaca
    21241 agccggcctg gccgccgtcg tgcccgcagg caagttttcc cccgaccggt tcttccgcgt
    21301 agtctcggat ctgcccccat atgcgcagcc gcgcttcgtt cgcgtgatga gcagcctcgc
    21361 gaagaccggg accttcaaga ttcagaagaa cgatctgcga aaagatggcg tcgatccgtc
    21421 atcgatcgac gacccgatct acgttcgaac ggctaacggc tatgagttgc tcacggaaga
    21481 tcgctgggtc gatgtcaagg aaggacgcct caagctctga tgccggagat gaatgcagag
    21541 cagattcaga gcttcatgaa aaattccttc cctcaggcgc ggatgcctgt cgagatcgag
    21601 gaactgcggg acggctacct gcggatccgc gttccggtct ccgatcggta ccttcgcccc
    21661 gggggcacgg tatccggccc caccttgatg acgattgccg acacggcgat gtactatctc
    21721 gtgctcggca tgatcggccc cgttgcgctt gcgctgacta ccaatttgaa catgagcttc
    21781 cttcgtgcac ccggactcgc cgatgtcatc gccgaaacga agatgctcaa gctcggaaag
    21841 cggctagccg tcgggcaggt gacgatctac tccgacggct ccgaagatcc ggtcgctcat
    21901 gccaccgtga cgtactcgat tcctccggat gctaagcgcc cttcaacgcc ttctcgatga
    21961 tggagcggtc ctccggggcg tactcgaact ggagcgcggc gtcgtgcgcc tgttcgctca
    22021 tcttcttcca ggtcttctgg acgatgggga gtagtttctc ctccgagtgc ttctgcgcga
    22081 aatcatggaa gtagtgttcg agaaaaacta ggcaaatcac gtcttccaga agctgcacct
    22141 cggggtcggt ctttaggccg cgcttccgca agagagtctg tactcgcatg acgagcgcat
    22201 cgtcgtagcc gacgtctttc atgatcgccc cggcgatttc gccatgccgt ttgtagaggt
    22261 cggtgcgcca tttgaggtaa cccgccctgc cctctggata gctcgagcgc ggaacctccc
    22321 aacggcgaat gtgctgagct cgcgcggcga gttgcaccgc gtcggaagcc tcgggcgcaa
    22381 agaccgaaag acgagccgac atgcgctgcc cgtagacgag ctccttgggc agcggctgac
    22441 catcgactag ctcggtgttc gggtcgtccg cgttcgccgc atcgaagcgc cgaagtgctt
    22501 catcgagagc tggcatgcga cgattgtatc atggcgacgc cgcttagcgc cgaccgttgc
    22561 tcagctatgg agaggcatcc ttggcggcgc cacgtgcggc agcgcagacc gatgccacag
    22621 acgaacctcc gaacaccatt tgaatgccgg cgagcgtgcc cacggcccaa acaccagaga
    22681 cgggaaactg acgccagatc atgacgccga gcgcgagcga aacgatgccg ctgaacagcg
    22741 cccagcccca gcctttgatc ggtcggagtc gaaacgccca gaggatttga aagacgccgt
    22801 cgaccgcaaa gtagaccgcc aagacgaggg tgagcgtccc tagcgccata ccagggcgcg
    22861 aaatcatgta cccgccgatg atgatggcga gcgcgcccag cacgaacacc agcagcccgt
    22921 gcccgaacga gcccattctg aacgcgagaa acaaacgcga aacgcccgag gcgatcagaa
    22981 gcaccccgac ggcaattgcg atcgcaaggc cggcgacgag cggcgagagc agcgccaaaa
    23041 tgcccgccac gacgaccagg atgcccacgg ccgttccgac acctgcatgc cgcttgatgg
    23101 tgccgagcat ctcctgatcg gcttctaaaa tcccgttcgt cataggtggc ctccaagggg
    23161 gcttatagga gcccggccta ggaaaacgca agcgaaaccg aggtggtatc gatgcccggg
    23221 ggcctcgact tgccatctct ctaccgtttg gtagacaaat ggcgaccaat tggtagggac
    23281 gatggatgta cgtgctcaga ttctaaccca tgcaacacgc ctgtttgccg aacagggcta
    23341 tgacggcacc tcggttcagc aaatcgcgga tgcggtggga attcggaagc cgagcgtgct
    23401 ctaccacttc aagtcgaagg acgagctacg cgaagaggtg ctctcggaga tgctcgccca
    23461 ttggaatacg gtcctgcctg gactgctctt gaaggcgtca gccgaagagc ggttcgacgc
    23521 gacgatggaa gcactcaccg aatttttcat cgtggatccg gaacgcgcgc ggctattcct
    23581 gcgcgaaacg ctcgaccgcc cggagcagat gcaatcgatg ctcggcgcgt tcgtgcaacc
    23641 ctgggtcgag ctgctgggcg atcagctgga gcgagcgaaa gcgcaatgct tggtccgccg
    23701 agacgtggat ccccatgctt atgccgtgga ggtaatcacg atggcggtcg caggtaccgc
    23761 tgtcatcgac accttacgag tcatcctgcc cgatgatccg gtccgcggca gcacacgaat
    23821 ccgacacgcg cgcgagttga ttcgcatcgc gcgctcgagc ctctacacag agaacgaagg
    23881 cgagaaatag tcatggccaa tttcttcaaa gacaatgacg atcttcagta ctacttcgac
    23941 aaggggatcg actgggacac ggtcgtgcgg atcacggagc acgagtttgc ggacgcggaa
    24001 ggcgacggct tcaaagatac ccaggaagcc gtcgaattct atcgcgagat agtggagatg
    24061 ttcggagagt tcgtagccga ggagatcaag ccccacgaga aggaaatcga cgaagaaggc
    24121 gtcgctctag taaacggcga ggtcgtgttc cccacccggc tcgctggaat cttcgagaag
    24181 atcaacgagc tcgacctgca cgggctgccg ctcccgcgag agctcggcgg gatgaacgca
    24241 ccgatgttgg tctacttcat gaacagtgag ctgatggcgc gagccgatgt atcgacgatg
    24301 gcgcaccatg gattccatgc gggtattgcc atggcagcgc tcgtattctc ggcaatcgag
    24361 ggcagctcca agatcgaccc caagaccggt cgcatcgacg agacccggtg gagacacgtg
    24421 atcgaagaga tcgccgccgg caaagcctgg ggctgcatgg acatcaccga gcccgatgcc
    24481 ggttcggaca tggcggcgct tcgcgctgtc ggcgaacagg acgcagatgg aaactggcat
    24541 gtcacgggcg agaagatctt catcacttca ggtcacggga agtatcactt cgtaattgcg
    24601 cgcacggaga agagcgaccc cgacgatccg atggacgggc tcaatgggct atccatgttc
    24661 ttagtgcccg cttacgagga ggacgcagaa ggaaaccgtc ggcgaatcgt tcaactgagt
    24721 cgtgtggaag agaagctcgg gcaccatggg tcagcgactt gctcgctcgt gttcgacaaa
    24781 gctccagccg atctggtcgg tgaacgcggc gaagggttca agtacatgct gacgctcatg
    24841 aacaacgcgc ggttgggcgt gggcttcgag tgcatcggac tcagcgagtc ggcctaccgg
    24901 gtcgcgacac agtacgcatc cgagcgcgcc tcgatgggca aaaccatcga caggcacgaa
    24961 atgatcgccg agtacctaga aacgatgcga accgagatac agggcgttcg ggccatggcg
    25021 gtgacagcgt gcttccacga agaaatggcc cagaagctct cgttggccgt ccgttttcga
    25081 gacctcgacg caagcacacg gcagcagatg gagcgactca tgcggtcgca ccagaagaag
    25141 gctcgccgaa tcacgccact tctgaagtac ttcgccgcag aaaaggccgt cgagcacgcg
    25201 cgaacggcgc ttcagattca cggcggcaat ggctacacca ccgagtacct gccagagaag
    25261 ctgctgcgcg acgcgctcgt catgccgatc tacgagggca cgagccagat tcaggcgctg
    25321 atggcgatga aggacacgct cggagcaatc atcaagagcc cgcaggcctt cgtgcggcgg
    25381 cttgcacagg caaggtggcg atcgatctcc agccgcaacc cactcgaacg acgagtcgcg
    25441 caactgcaat cgctttcgct gagcgcacag cagcacttgg tgctgaagac agcgggagcg
    25501 aagataagag gtttgcgcgg gaaaccgatc ggggactggt cggacgatct cacgaaaaac
    25561 tggaatccca agcgcgactt tgcgtttgcc atgttgcacg cggagcggct gatccagatt
    25621 ctcggcgaca cgacaatcgc cgagcttctc ctggcgcaag ccgcgaagca tcccgagcga
    25681 gcagaagtcc tcgaacggca tctcgagcgc gcagagccaa gagcgcgcta cctgctcgat
    25741 cagattacga caacgggcca gcgcttgttg gaaaaactgt caccggagtc ggcggaggcg
    25801 atgggccagg ccgcggagtg agcgccctca acgcggatcc cacggattcg agcctctaat
    25861 accaatcgtt cgcctggtac cactcgtgcg tgaggcggat gccctcggaa agccctattt
    25921 gggcctcaaa cccgaaatcg cggcgcgccg cgtcgccgac acaggtccac gcctgctgcg
    25981 cacccatctt cgctttctgc aagttcagaa gtctgggctt cttatcgacg cgcgtcgcaa
    26041 gctcgcctcc gagcgccgcg acggtgacca gctgtgaagg caagttgatg gtccgcactc
    26101 ggcgatccac gacccggagt atctccgact ggaactgctc ccagctaacc tgctgttcgc
    26161 tggtcaagaa atagcccttc gcctcgctgc tcgggtgctg ggccccctcg aggattccgc
    26221 gaacgcagtc atcgacgtag atgacggaca tgagccggtc gcgattgccg aagaacacat
    26281 tccaaccgcg catggcgctc ttgaacaagt tgaaatagtc gacgtccccg gggccgtaga
    26341 cgcccgaggg ccgtagaatc gtccacggga tgccggcaga ctcctcttcg accactcgct
    26401 ccgcttcgag cttgctctgt ccgtagtact cgatcggccg cggtgggtcc gcttctcgat
    26461 gaggggcaga ggtcgcagac ggaccgtacg aagtcagcga tgacaccaag acgaagcgct
    26521 tgacgtccac gtgcgccctg cccagcgctg tgagcagatt tctggtgggc atcacgttgc
    26581 ccgtctggaa gtcctggtac gagacgccct tcgtcacacc tgccacatgc aagacatagt
    26641 cgggcttttc atcgccgacg atgcgctcga gatcctcgag acgagcgtaa tccgcctgta
    26701 cggagcggcc tcccttggac gggggcgagc cgggtcgacg aatcgcgacg acgtcgcttc
    26761 cggccgcgat gagtgcagcg cgtaatcggc ttccgatgaa gccgctcgct cccgtgatca
    26821 ataccttgcc gtctggtttg ggcccagcca ttcgcccgtc ctgcctcgca agcggccgat
    26881 gtgcaagccc ggattgacgc ggaccaactg ctgggaaacg ctatgagcca tgactggtag
    26941 cttcaagacg atctctccag tggacggcag cgtcgtgttt gaacgatcgc tcgacggacc
    27001 aaaagaaatc gaaggaacac tggctgccgc cagcaaagcg ttcgaatcat ggcaccaagt
    27061 ggcgctcgaa gagcgctgta cgatcgtcag gcgcttcgtg gagctcgcgg tgcacgacac
    27121 cgatgccgtc gcgcaagagc tgacgatgca aatgggccga ccgattcgtg acggtggggg
    27181 tgaggcgggg ggttggttgc ttcgtggccg aacgatgatc gatctggctc ccgaagccct
    27241 ggccgacatc aggctggaag cgcgcgacgg gttcacccgg ttcatccgcc gcgaggcact
    27301 tggcgtggtg ttcgtcgtcg cgccatggaa ctacccttgg ctcaccgccg tgaacgcgtt
    27361 ggtgcccgca ctcgtcgccg gcaactcggt ggtcctcaag cactccgagc aaaccccgct
    27421 cgtcgcagaa cgaatcaacc gggcttccag ggaagccggc ctgccgaagg gcttgctcca
    27481 agttttgtac ctggaccatg cgggcgtagc agacgtgatc gcagacccgc gcatcgcgtt
    27541 cgtggcgttc accggctccg tcgaaggtgg gcacgcgatt cagcgagcgg catccaaacg
    27601 cttcattggc gcagcgctcg agctcggagg gaaagacccg gcctatgttt gcgcggatgc
    27661 tgacgtggcg cggtcggcag caaacctcgt cgatggctcg ttttacaacg cggggcagtc
    27721 ctgctgtggg attgaacgaa tctacgtgca tcgcgaggtt tacgatacat tcctcgaagc
    27781 gttcgtttcg gtcgcgaacg agctcgtgct cggtgaccct cgcgattcgt tgacgactct
    27841 cggaccggtc gtgcgagttc gcaacgccga aagcatccaa gcccaaatcg acgaagcgct
    27901 caccgcggga gcgcgcccgc tgctcgaccc caagcacttc caggcttcca agctcggcct
    27961 gccgtacatg gcgccacagg ccctggtcga cgtggatcat tccatgagaa tcatgaccga
    28021 ggaaaccttc gggcctgccg tcggaatcat gagggtagaa gacgatgcag aggccatcgc
    28081 gatgatgaat gatagcgcct acgggctcac cgcatccgtt tggacgagtg acctggacca
    28141 tgctcaagag atcggttccc gagtagacac cggaacctgg ttcatgaacc gctgtgacta
    28201 cctcgacccg gagcttgcat gggtgggggt gaaagattcg ggacgaggtt gtagcctatc
    28261 taccctcggg tatgcgcagc tcactcggcc gaagtcgttt catctccggc atgctccagg
    28321 gtagcctggt caacggcgct cgggtcgcta tagtcggcct cgatgaaacc gccggtgctc
    28381 cgctgccgtc acgtcacaaa gcgctacggg aagttcgagg ccttgaagga cctctcgctc
    28441 gaggtctacg aaggggagat cttcgccctc ctcggcccca acggcgcagg aaagtcgagc
    28501 ctcatccact gcatcaccgg gctttccaag cagaccgacg ggagcatcga agtcctcgga
    28561 ttcgacacgt tgaacgattt tcgaacgaca aggcgcttgg ttggcctcgt gccgcaagag
    28621 atcaactacg acccgttctt cactccgctc gaaagcctta tgattcaaat gggcttgatg
    28681 ggtgtcgagc ccagccgggc gcgcgcgctc agtctcctcg agacgttctc cctcgacgag
    28741 aaacgtgacg cgtacacgcg tacgctctcg ggcggcatga agcgccgcct gctcgtagcg
    28801 aaggcgctcg ttcacgagcc acgtttgctt tttctcgatg agccgaccgc gggcgtggac
    28861 gtcgagctcc gtcgcgagct ttgggacgag gtcgaggcgc tgcgtatggc gggaaccacg
    28921 atcgtgctca ctacgcacta catcgaagag gcagagaaac tcgccgaccg aatcggcatt
    28981 ttgcacaagg gggagctgct cctggtcgag agccgcgaca gcctgatgga gcgccacggc
    29041 ggacgttcgc tcgaagacat ctacctggag ctcattcaaa aggccgatgg aaggcatgcg
    29101 gcatgagcac gatcgcgctc aagacgctct tcctgaaaga aattcgccgc ttcagtaaag
    29161 tctggttgca gaccgtgctg agcccgctgg tgacgacgag cctatacttt ctggtgttcg
    29221 gcgtcgcact cggttcacgc cttcgagaga tttccggcgt gccctacatc cagttcgtcg
    29281 tgccggggct cgtcatgatg accatggttc gagactcgtt cctaaacacc tcttcgagcc
    29341 tgtttcaatc gaagatcaac ggaacgatca tcgatatcct cgtcgccccg atcggcgcac
    29401 tcgagatgct gctcgcatac gtcggcgccg cgatgctccg agcgatgatc gtgggctttc
    29461 tcgtctacct cgtcgccctc actttcacct ggttccccct tcatcacctc ggttggattt
    29521 tgtttttcac catattcgta acggccaccc tcgcactcct gggcatggtc gcagccctct
    29581 gggcccagaa gttcgaccat ctggcgatct tcccgaattt cgtattgctg cccctcgcat
    29641 tcctaggtgg cgtcttctac tcgatcgaca tgctgccgga gccctggaac acgataagcc
    29701 gcctaaaccc acttctttac atggtgaacg gtcttcggta cggctttctc ggcgtcgctg
    29761 acgtttcacc cggcgcgagt gccgcgatcg tcgtgatttc gttcttcgcg gtgcttggcg
    29821 ttgccagcgc cctcttgcgc agtggctaca acctgagaac ttagtcacca tgagctccct
    29881 ggcatgagca gcagcaccta cttcgcagac cctgcagccc gcgcattcct ccagcgtcgc
    29941 gtcgccgcgt tcggactgat cggtgcgctc ctcggcggca cagccctgct gtttcgcatg
    30001 atcatgggca tcctgttcgg cttcatccgc cagcagataa ccgaccccgg gttcttgatt
    30061 cacgccgctg cgatccttcc actgctcgga atttggctga tttgccggcg cgggaagctc
    30121 tcggtgaatg ccattcacgc ggtcgagaac actggcattt tcttggcgag cgtttgctac
    30181 atcggcatgg ggatggacat ccgtgccgag gttggcgccg atacgatcac cgcgttcgtc
    30241 ttggcgctga tgatgttcgc acgaagcgtc tacgtgccga gctcggcgaa gcgaacgacg
    30301 attcttggga ttctgatcgg cgtccccttg gtcgcggcga tgtactacca ctacttgggg
    30361 gtcgacctgc gcatctggcg gagcttcggc tacgaaggtc aatcggtgca gcgggtcgcg
    30421 acatcgcaag cgatcatcac gaccatgtgg tggaccctca ctacggggct cgcggcgctg
    30481 gcgtctcgtg tcatccaccg tttgcgcaag gaggtcagcg acatccaaag cctcgggcag
    30541 tacaagctgg agcgcaagct cggcgaaggc gcgatggggg tcgtctacga ggccacgcac
    30601 gggatgctga agcgtccgac ggcgatcaag ttgctgcgcc ccgagctagc cgatccggac
    30661 gctttggagc gcttccgcag agaggttcag ctgaccgcca agctcactca ccccaatacc
    30721 gtcacgattt acgactacgg tcgtacaccc gaggggctct tctactatgc gatggaactt
    30781 ctgagcgggg cgacgctcac gcaagtggtc acagctagcg gaccacaacc agtcgagcga
    30841 gtcgtgcgca tcctccgcga tgcggccctt gcgctgaacg aggcccacga cgtcgggttg
    30901 atccatcgcg acgtgaaacc gagcaacatc atgctcgcgc gccagggcgg cgtccccgat
    30961 gtgacgaagg tgctcgactt cggtctcgtg aagaacctcg gcaaggtcga cgacctcgag
    31021 cagaccaata cgatgtcgat caaaggtacg ccgcactatt tgagcccgga ggcgattcaa
    31081 gaccctcagg gaatcgacgc gcgcaccgat ctctacgcgc tcggcgcggt tggctactac
    31141 ctactggccg ggcgtcacgt cttcgacggc aagacgatca tggaggtctg ccttcaccac
    31201 ttgcagacca agcccgtttc cctggccgag gtctgccaaa ccgaggtgcc cgctggtctc
    31261 gaagagctga ttttcgcctg cctggaaaag cggcaggccg atcggcctgc gacggctcag
    31321 gagctagccg acgcgctgga cagactcaag gtcgcgcgct ggactcgggc cgatgcggag
    31381 gcctggtggg acgtttttgg ccaggaaatc gagaaagcca agcagccgtt gtcgagctca
    31441 ggcaccgaac agacgctcgc aatggacctg gacgagcgct gattgtccgg caaacacggc
    31501 ggtgtatatt tgatgcattc gtcttgcttg attggatcct ccacgtgaaa gcctctgtct
    31561 cagctctcgc gctctcgatc gcggtgctct tgggaagctg cagctcggac tcgcaggtcg
    31621 gagtggcgca gaacatcttc gctgatctcg gcgctccgct cccttccgca acagccgcag
    31681 agcttcgaag cttcgagcgt ggtcggaccg ccgcgcttcg tcggtttacc ccctcagatg
    31741 gactcggccc ggagttcaat ctcacgttct gcgccggctg tcatgagaag cccgtctttg
    31801 gcggaggcgc cagccattac cgggatttcc ttctggtcgg cgatgagctc tacccagaca
    31861 cggtcgtgcc gcgcggcaaa aacggggtgc agcgacaatt ctcgctcgaa accggccgcg
    31921 ccccttccga cccgctgacc aacatcagcg caactcgaaa tccgattccg ttttttggtg
    31981 caggacttct cgccgagatt ccggacaccg agatcgtgag ccacgccgat cccgatgact
    32041 cggacggcga tggaattagc ggccgcgtga actacgacgg cgtgctcgtc ggacgtttcg
    32101 ggcgaaaagc gcaaacctcg agactcgagt tgttcgtccg gggccccctc ttcaatcacc
    32161 tggggattac ctcggaaccg ctctccgaag cgcggcgggc agagctccga agctttagtg
    32221 cgtcggccgg cgtggtaact gcgattccga aacaagctgt gatcccggac gagcccaccg
    32281 tcgacctcga tgatgtcccg gaccctgagc tctccgaaga cgagctcttc gacctcgtgg
    32341 catttgcgtc gttgctcgct gcaccgagac cggacgagtc gaccgacgaa accgagcgcg
    32401 gagcaatcac gttcagcgaa gtcgggtgcg atcgatgcca tctcccctcg ttgagtggtc
    32461 cgcgcggaag catcccggcc tacaccgact tgctcctgca cgacatgggt gacgagctcg
    32521 cggatcgctt tccgatgggc caagcgacgg gccgagagtt tcgcactcag ccgctctggg
    32581 gaatcgcggc ggccagcccc tatctgcacg atgggcgcgc agcgacgctc gacgaagcga
    32641 ttcaatggca cggcggtgaa gcaaccgaga ttcgggatgc ctatgcagcg cttccagccg
    32701 gcgggcgtgc agatctgatc gcatttctgg agtcgctcgg cggcagggct cagtcgagcc
    32761 caggcttgat gccccccgat gccccagtac caagccaagg cgagtacggc gcgccagtag
    32821 tcgggctgag cgcggaagag caggctctct tcgagcaggg ccgcttactt ttcgacaggg
    32881 acttcagttt ctctgaaggg gtgggccctt cgttcaacgg ggacgcatgc cgatcttgtc
    32941 acttcgatcc ggtaatcgga ggcgccggcc caagtggcgt ggacgcaatg cgccagggac
    33001 gcgtcgaagc gttcatcttc agcgcccctg acggaggcac cgccatacca cgccacgcgg
    33061 taaacggggt gcggcctgaa gcggatccgt cctcgaactt cttcgagcgg cggcagacgc
    33121 cctcgacgct cggattgggc ctgctcgaac aggtcccccg cgagacgatc gaggccctcg
    33181 cagatcccag cgatgagaac gacgatctga tctcgggacg ggtgcacatt ctcgacgacg
    33241 gacgcatggg gcgattcggc tggaaggcca acgtgccatc ggttcgcgag ttcgtgcggg
    33301 atgcgttcag cggcgagcta ggcgtcacgg tgcctgaaga gccaggtttc acgttcggtc
    33361 tcgccgcgga cgacgacggt gtggccgacc cggaggttga tgtcagcagc atcgacgcga
    33421 tcaccgcgtt catccagttc ctcgatgcac caccacgaga tcgcagtgac gagacactgg
    33481 aagatcgagg cgaggcgatc ttcgacgtcg tcggctgcgc tcagtgtcac gtcccgaatt
    33541 tggaaacacg ggacggcacc gagctgcacg cctacacgga cctccttctg cacgagatcg
    33601 ccaaagcgag cgatttggga atcgaagaag gcgatgctgg cattcacgag ttccgcaccc
    33661 cgccactctg gggcctcagc cggacggccc cttatctgca cgacggccgg gcaagcacga
    33721 ttgaagacgc gatcgcagag cactccggtg aagctttgac ggttcgcggc gcgtttgaag
    33781 cgctgagccc cgcagaccag gatgcgctcc tcgccttcct gaggtcgcta tgagcaagtc
    33841 gctcgccgtt ctggcgctcg cgctgatctt cggctgtggg gacggcgagt cgctgcagcg
    33901 cgcagagctc ttggactcgg agggccaggt actgctcggc ctccgagtcg cgatcgcgga
    33961 cacggaatat gaacgccaag aaggac
//
LOCUS       DASRZF010000328         9804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_5063521, whole genome shotgun sequence.
ACCESSION   DASRZF010000328 DASRZF010000000
VERSION     DASRZF010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 9804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9804
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5063521"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VFG22_14505"
     CDS             <1..679
                     /locus_tag="VFG22_14505"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HET6417506.1"
                     /translation="LLKRQMVNQNPAAGLRSPKIAKTLPRFLSVDQAFRVMEAPPKED
                     KRAKPLKARDQAMLETLYGTGVRVGELAGMNVEHCDFSDAAVRVLGKGGKERIVPLGK
                     AALQALQAYLPERRGLLQKAKAAEPDALWLSQGGRRLSVRQVQNIVRRHGTQGAGRGD
                     LHPHAMRHSCATHLLDAGADLRSIQELLGHSSLSTTQRYTHVSVDHLMEVYDKAHPLA
                     RDKSGEG"
     gene            717..1271
                     /gene="hslV"
                     /locus_tag="VFG22_14510"
     CDS             717..1271
                     /gene="hslV"
                     /locus_tag="VFG22_14510"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013939585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HET6417507.1"
                     /translation="MHQPDPPVFRSTTIVAVRRDGKAAMAGDGQVSLGQTIMKGHAKK
                     VRRIADGKVVAGFAGATADAFTLLDRFEAKLKEHRGSLQRSAVELAKDWRTDRYLRRL
                     EAMLVVMDAESTLLISGTGDVINPDEGVIAIGSGGSYALAAARALLRNTDLSASDIAR
                     RSLEIAAEICVYTNDEIIVEEMGG"
     gene            1268..2683
                     /gene="hslU"
                     /locus_tag="VFG22_14515"
     CDS             1268..2683
                     /gene="hslU"
                     /locus_tag="VFG22_14515"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003740291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HET6417508.1"
                     /translation="MTLGNFTPRETVGELDRYIIGQHKAKRAVAIALRNRWRRQQVAG
                     ELRDEIAPRNIIMIGPTGVGKTEIARRLAKLARAPFVKVEASKFTEVGYVGRDVESMI
                     RDLVENAIQICEKEALEGVQTRAQDAAEERLARMVLAAQRPPTQTQQSQPSPQTHQFG
                     PLSFQITPQQAPQPQAFYSDSEVNDMRQRLRNGELDEIEVELDVTDTQNPFMTIFGAQ
                     GTEEVNLQDMMSQMPGFKGRTKRRRVKVPDALEILQQQEAKKLIDHDKVQREAVQRTE
                     QAGIVFLDEIDKVGSDRSTGGPDVSREGVQRDLLPIVEGSTVSTKYGTVKTDHILFIA
                     AGAFHVSKVSDLIPELQGRFPIRVELDSLEREDFVRILTEPKNALTRQYQALMATEGI
                     EVVFEAEAIEELAGFAAEANRRAENIGARRLYTIMEALLEELSYDASERAQSKFIVDV
                     EYVKSTLDPILANEDLARYIL"
     gene            complement(2696..3334)
                     /locus_tag="VFG22_14520"
     CDS             complement(2696..3334)
                     /locus_tag="VFG22_14520"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HET6417509.1"
                     /translation="MNRYEQVFENNRRWVEEKTAHDPEFFRKLAGGQDPDFLFIGCAD
                     SRVPANEIMGLNPGDVFVHRNVANLVVSTDINAQSVIEYAVAHLDVKHIVVCGHYGCG
                     GVAAAMQPADLGLLNGWLREIRDVYRLYQAELDAIKDENARYRRLVELNIQEQCVNVI
                     KTAVVQKTYLRTGHPTVHGWVYDLANGILHDLKIPFGEILGKIQEIYRLEPS"
     gene            3415..4845
                     /locus_tag="VFG22_14525"
     CDS             3415..4845
                     /locus_tag="VFG22_14525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HET6417510.1"
                     /translation="MPLHEQLTREIRTGPSGPSIGAFFDLDQTLFAGFSATAFTRDQF
                     SSGRLSPRDLRDSLRATLSFALGRTGFSTFVSATSAVYRGMQESVLEEAGQLTFEKHL
                     ATQIYPESRALVEAHQAMGHTVAIITSATRYQAAPVARELGIEHLLYTQLGVENGVLT
                     GEVVRPTCYGEGKAAAARSLESKYGLDLDQSYFYSDSHEDLPLFEIVGRPRPLNPNRK
                     LAQIAKERQWPVRRFTSRGTPTFGDVIRTGLIYGSLAPAVAFGTAAGLLNRSKREAFN
                     VMGSVWGDLAASAAGIDLRVEGQEHLWSHRPAVFIFNHQSGLELVLLLKLLRRDFTGI
                     AKQELRRNPIFGPLFSAAGVVFIDRTNTVKAIQALEPAVEALRQGRSLIIAPEGTRST
                     TALPGPFKKGAFRLAMEAKVPIVPVVFRNVLDALPKGALIVRPAMIEAVVLPAIDTST
                     WTLDTLDEKIESIHDRYLAILSGAEA"
     gene            complement(4873..5172)
                     /locus_tag="VFG22_14530"
     CDS             complement(4873..5172)
                     /locus_tag="VFG22_14530"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET6417511.1"
                     /translation="MEEPSWTFLTNHSHVLLCLVQDPEARMRDVAERVGITERAVQRI
                     VSELESAGYVSRTREGRRNTYEVNGTLPLRHPIEGHQSLDALIKLILKTRPRTKH"
     gene            5377..5886
                     /gene="purE"
                     /locus_tag="VFG22_14535"
     CDS             5377..5886
                     /gene="purE"
                     /locus_tag="VFG22_14535"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HET6417512.1"
                     /translation="MKVNPESEPESPPRVAVIMGSQSDWSTMQEAARVLAELGIPHET
                     KVVSAHRTPDLLFSYCEQAEARGIEVIIAGAGGAAHLPGMAAAKTLLPVLGVPIKSSA
                     LNGIDSLLSIVQMPKGVPVGTLAIGEPGAVNAALLAAAFLARGDAELRDRLQAFRSAQ
                     TARVLEAEL"
     gene            5883..7067
                     /locus_tag="VFG22_14540"
     CDS             5883..7067
                     /locus_tag="VFG22_14540"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004676.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HET6417513.1"
                     /translation="MSAILPGATIGIIGGGQLARMMVLEARRMGYRVCVLSPDPSAPA
                     VSLADSWIRGNIDDVAAGERFAHSVDVITVDTEHVPASLLAQLELLRPVRPSPQVLRT
                     VQDRHAQRLFLDAVDAPQPVCVAVDSLPELFHEADRVGFPCVLKSRHAGYDGKGQTVV
                     RERGGLASAWANVGSTGAMLEELIDFDFEISVLLARNPGGEVRFYPIAHNVHRHHVLH
                     ATMAPARIDTAVEHEAKRIANRIANALDHVGMMAVEMFVVDGTRLLVNEIAPRPHNSG
                     HYTFGACVTSQFEQHIRAIADLPLGDPSLPRPAVMVNLFGDLWTDGVPDWTPVLSKPE
                     VRLHLYGKAEPRPGRKMGHVLVLDPDPYRALRTGEALLDGLRARCADGPKSEIENSTC
                     VS"
     gene            7129..8547
                     /locus_tag="VFG22_14545"
     CDS             7129..8547
                     /locus_tag="VFG22_14545"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HET6417514.1"
                     /translation="MKISVAMGDGIGPEIMGTVLRVFEKASVPLEYELVEMGKEVVLS
                     GEQTGISASARQSVESSGILFKGPMETPQGGGYKSVNVTARKLWGAFANKRVYRSFPG
                     VPAPIGSRHLDLTMVRENIEDTYGAVEHMQTHDVAQCRRFITRPGSEQVHRYTFEMAA
                     RKGARHVTCAHKANIMKLTDGLFLEVFYDVAKDYPEIEADDVIVDALAMRLVLDPSEF
                     EVIVLPNLQGDILTDLAAGLVGGLAYAPSANIGDGICIFEAVHGTAPDMVGQDLANPS
                     ALVLSGTMMLRHLGLVPHATAIEKALEQTLRSMHKRPDLGQPIPVFRTSVFEEKMLDQ
                     LDAVHIPEGELPRMEVLRRTEPKMRISPTPEHTVLRGVDVFLESGLPPKLVEAQLNEI
                     GGWLKLTMISNRGTQVAPTGSAYTDCVNHYRARFETQQPVSQESIVSQLAAVGERFRL
                     CGAEWLREIDGKQGYSLAQGQS"
     gene            8594..9463
                     /gene="argB"
                     /locus_tag="VFG22_14550"
     CDS             8594..9463
                     /gene="argB"
                     /locus_tag="VFG22_14550"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HET6417515.1"
                     /translation="MDHLIEKAATLQEALPYIREFHGDIFVIKYGGHAMVDPALRDSF
                     ARDVVLMKYVGLHPVVVHGGGPQIDQMLKSLGVVSERLDGLRVTDDHTMEVVEMVLGG
                     KLNQEIVSLIAGHGGRAVGLTGKDDAFLRATKVDKMQTKSGKWVDPGRVGVVSQVNPE
                     VLRRLVDDGFIPVIAPVAVDAQGKSLNVNADTVAGKIAEALPARKFVLLTDIEGVRGK
                     DGEVASSLSAAQIDALKADGIIEGGMIPKVDCALSALAGGVRKVHIIDGRVRHAVLLE
                     IFTDEGIGTEITR"
     gene            9532..>9804
                     /locus_tag="VFG22_14555"
     CDS             9532..>9804
                     /locus_tag="VFG22_14555"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET6417516.1"
                     /translation="MTRQDDMLRTAKEVQLGNYAPAPFVLTHGRGRRVTDASGREYLD
                     LSGGISVLSVGHSHPTLAAAIGEQAARLMHVSNVFYNDRAIELAEAI"
BASE COUNT         1929 a         3019 c         3048 g         1808 t
ORIGIN      
        1 cctgctcaag cggcagatgg tgaatcagaa tccggctgcc ggcttgcgat cgcccaagat
       61 cgcgaagacc ttgccgcggt tcctcagcgt cgatcaggcg tttcgcgtca tggaagcccc
      121 gccgaaagag gacaaacggg caaagccgct caaggcgcgg gaccaggcca tgctcgaaac
      181 cctgtacgga acgggcgtcc gcgtcggtga gctcgcaggg atgaacgtcg agcattgcga
      241 cttcagcgac gctgcggtgc gggttctagg caaaggcggc aaggagcgca tcgtcccact
      301 cgggaaggcg gcccttcagg cgctgcaagc ttacttgccg gagcgtcgcg gtttgctcca
      361 aaaggccaaa gccgccgagc cggatgcgct atggctgtcg caaggtgggc gaaggttgag
      421 cgttcggcag gtgcagaaca tcgttcgacg acatggcacg cagggggcag ggcgaggtga
      481 tctacaccct cacgcaatgc gccatagctg cgcgacgcac ctgctcgatg ccggcgccga
      541 ccttcgctcg attcaggagt tgctggggca ctcgagcctt tcgaccactc agcggtatac
      601 ccatgtgagc gtcgatcacc tgatggaggt ctacgataag gctcatcccc tggcgcggga
      661 caaaagcggc gaaggttgac agcccaaaag gccgacttag gttccttcga ctcaatatgc
      721 atcagccaga tccgcccgtt ttccgctcca ctaccatcgt cgccgtgcga cgagacggta
      781 aggccgccat ggccggagac ggccaagtct ccctcggtca gacgatcatg aagggtcacg
      841 ccaagaaggt ccgtcgcatc gccgacggca aggtggtcgc cgggtttgcc ggtgcaaccg
      901 ccgacgcctt cacactgctc gatcgcttcg aggccaagct caaggagcac cggggcagcc
      961 tgcagcgttc ggcggtagag ctggcgaagg actggcgcac cgaccgctac cttcgacggc
     1021 tcgaagcaat gctcgtggtg atggacgcgg agtcgacact tctcatcagc ggaacgggcg
     1081 acgtcatcaa cccggacgaa ggcgtcatcg ccattggctc aggcggttcg tacgctctag
     1141 ctgcggcacg cgcgctattg cgcaacacgg acttatcggc ttctgacatc gcccgccgct
     1201 cgctcgaaat cgccgcggag atctgtgtgt acaccaacga cgagatcatc gtcgaggaga
     1261 tgggcggatg acgctaggaa acttcacgcc gagggaaacg gttggcgagc tcgaccgcta
     1321 catcatcggg cagcacaagg cgaaacgtgc cgttgccatc gcgcttcgaa accgctggcg
     1381 gcgtcagcaa gtagcgggcg agctacgcga cgagatcgca ccccgcaaca tcatcatgat
     1441 cgggccgacg ggggtcggta aaacggagat tgcgcgcagg cttgcgaagt tggcgcgtgc
     1501 ccccttcgtc aaagtcgagg cgtcgaagtt caccgaggtc ggttacgtcg ggcgcgacgt
     1561 cgagtcgatg atccgcgact tggtggaaaa cgccattcag atctgcgaaa aggaagcact
     1621 cgaaggcgtg caaacgcgcg ctcaagatgc cgccgaagag cggctagcgc gaatggtttt
     1681 ggcagcccag cgtccgccca cgcagacaca gcaatcccag ccgagcccgc aaacgcatca
     1741 gtttgggccg ctgagctttc agatcacgcc gcagcaggcg ccacagcctc aggcgttcta
     1801 ctccgacagc gaggtcaacg acatgcgtca gcggctccgc aacggggagc tcgacgagat
     1861 cgaagtcgag ctcgacgtca ccgatacgca gaaccccttc atgacgatct tcggcgccca
     1921 aggcaccgaa gaggtgaacc tgcaagacat gatgtctcag atgccggggt tcaaggggcg
     1981 caccaagcgg cgtagggtga aggttccaga cgccctcgag attttgcagc agcaggaagc
     2041 gaagaagctc atcgatcacg acaaggtgca gcgcgaagcg gtgcaacgca ccgagcaagc
     2101 tgggatcgta ttcctcgacg agattgacaa ggtgggttcc gaccgcagca cgggcggccc
     2161 cgacgtatcg agggaaggtg tgcagcgcga ccttcttcca atcgtcgaag gctctaccgt
     2221 gagcacgaag tacggcacgg tgaagacgga tcacatcttg ttcattgccg ccggcgcgtt
     2281 ccacgtctcg aaggtctcag acctcattcc cgagcttcag ggccgctttc ccattcgagt
     2341 cgagctcgat tccctcgaga gggaggactt cgtccggatc ctcaccgagc cgaagaatgc
     2401 gctcaccaga cagtatcagg ccttgatggc taccgaagga atcgaggtcg tcttcgaggc
     2461 tgaagcgatc gaagagctcg ccggattcgc ggctgaagcc aatcgacgcg cggagaacat
     2521 tggagcgcgc cgcctctaca ccatcatgga agctctgctc gaggagctct cctatgacgc
     2581 atcggagcgc gcccagtcga aattcatcgt cgatgtcgag tacgtgaaga gcaccctgga
     2641 cccgatcctc gccaatgagg acctggccag gtacatcctc tgagcttcgc tcgcctcagc
     2701 tcggttcgag gcggtagatt tcctgaatct ttccgagaat ctcgccgaat ggaatcttga
     2761 gatcgtgcaa gatgccgttc gcgaggtcgt acacccagcc gtgcacggtg gggtgacccg
     2821 ttcgcaagta ggtcttctgc acgaccgcgg tcttgatcac gttgacgcac tgctcttgga
     2881 tgtttagctc gacgaggcgc cggtaccggg cattctcgtc tttgatcgcg tcgagctcgg
     2941 cttggtagag ccgatagaca tcgcggatct cccggagcca gccattgagc aggccgaggt
     3001 cggcgggttg catggcggca gccacacctc cgcacccgta gtggccgcaa accacgatgt
     3061 gctttacgtc caggtgggcg accgcgtatt cgatgacgga ctgcgcgttg atgtcggtgc
     3121 tcacgaccag attcgcgacg ttccgatgca cgaacacatc gcctggattg agtcccatga
     3181 tctcgttcgc gggcacgcgg ctatcggcgc atccgatgaa caagaagtcc gggtcctgcc
     3241 cacctgcgag cttcctaaaa aattcggggt catgcgcggt cttctcttcg acccagcggc
     3301 ggttgttctc gaagacttgc tcgtatcggt tcatgttccc ctcatgtctg ggtgtacatc
     3361 ttcgaccgcg actcttatca caaccgagcg agtactgtat gctctggggt ccacatgcct
     3421 ctccacgagc agctgacgcg tgaaattcga acgggcccct cgggtcccag tatcggtgcg
     3481 ttcttcgacc tggatcagac cctctttgcg ggtttttcag caaccgcgtt tacccgcgat
     3541 cagttttcgt cggggcgtct ctcgccacgg gatctccgcg attcgctgcg agcgaccctc
     3601 tcatttgccc tcgggcgtac cgggttttcg actttcgtct ccgcgacctc ggcggtctat
     3661 cgcggcatgc aggagagcgt tctcgaggaa gcgggtcagc ttacattcga aaagcatttg
     3721 gccacgcaga tctacccgga gtcacgcgca ctggtcgaag cgcatcaagc catgggtcac
     3781 acggtggcga tcatcacgtc ggcgacgcgc tatcaagccg ccccggtggc ccgggagctc
     3841 ggcatcgagc acctgctcta cacgcagctc ggcgtcgaaa acggagtgct caccggagaa
     3901 gtcgtgcggc ccacctgtta cggagaagga aaagccgccg ccgctcgcag tctcgagtcg
     3961 aagtatggtc tcgacttgga tcagagctat ttctattccg atagtcacga agatcttccc
     4021 ctgttcgaga tcgtagggag gccgcgcccg ctcaatccca accggaagct cgcccaaatt
     4081 gcgaaagagc ggcaatggcc cgttcgacgt ttcacgagtc gaggaacccc gacgtttggc
     4141 gacgtcatcc gcacgggtct gatttacggc agcctcgctc ctgcggtcgc gttcggaacc
     4201 gccgcagggc ttctaaaccg atccaaacga gaagcgttca atgtcatggg atccgtctgg
     4261 ggagatctcg ccgcatcggc cgctggaatt gacctccgag tcgaaggcca ggagcacctc
     4321 tggtcgcacc gccccgcggt gttcatcttc aatcaccaga gcgggctgga gctcgtcctc
     4381 ttgctcaagc ttctccgacg cgatttcacg gggatcgcca agcaggagct tcgtcgcaat
     4441 cccatctttg ggcctctctt cagcgccgca ggagtcgtgt tcatcgatag gacgaacacg
     4501 gtgaaggcga ttcaagcgct cgagccggcg gtcgaagcgc tgcgtcaagg tcgctcgctg
     4561 atcatcgcgc cggaaggcac gcgctcgacg accgctctgc cgggcccttt caagaagggg
     4621 gcgtttcgac tggccatgga ggccaaggtg ccgatcgtcc cggtcgtgtt ccgcaacgtc
     4681 ctcgatgcgt tgccgaaggg agccctcatc gttcgtcccg ccatgatcga ggccgtggtt
     4741 ctcccggcca tcgacacctc gacctggacc ctcgatacac tcgacgagaa gatcgagagc
     4801 attcatgatc gctatctcgc catcctcagc ggcgcagagg cctgacgagt tacattcgta
     4861 aacgaaggcg cgttagtgct tggtgcgcgg ccgcgtcttg aggatgagct tgatcagagc
     4921 gtcgaggctc tggtgacctt ctatggggtg tcgcagcgga agggtgccgt tcacctcata
     4981 cgtattgcgg cggccctcac gagtgcgcga cacgtagccc gcggactcca gctccgacac
     5041 gatgcgctga acggcacgct cggtaatgcc cacccgttcg gcgacgtcgc gcatccgggc
     5101 ctctggatcc tgcacgaggc agagaagaac gtgtgagtgg ttcgtgagga acgtccagct
     5161 gggttcctcc acctgattct ttgcctttgc caagctcgtt gccccgcgct cacgaacggt
     5221 tccgcaccga cgcaggagga gcatatacga cggccatttc gtgagtcaat tcggcatcaa
     5281 gccgatttct atggcttttt ccgactcggg gccttgcgct ttcggcacac tggagatata
     5341 tgaaacacga aatctacttc gcgaatgaga tagcgcatga aagtcaatcc cgagtccgaa
     5401 ccggaatccc cgccgcgagt tgccgtgatc atgggcagcc agagcgactg gtccacgatg
     5461 caagaggccg cgcgggtgct cgcggagctc ggcatccctc atgagaccaa ggtcgtgtcc
     5521 gcgcaccgaa ctccggacct cctattcagc tattgcgagc aggccgaggc aagaggcatc
     5581 gaggtgatca ttgccggcgc aggaggggcc gctcacctgc cgggaatggc cgcggcaaag
     5641 acgcttctgc cagttctggg agtgccgatc aagagcagcg cattgaatgg aatcgattcc
     5701 ctattgtcga tcgtgcaaat gcccaagggt gtgccggtcg gcacgctggc cattggtgag
     5761 cccggcgcgg tcaacgcggc actactagcg gctgcatttc tcgcgagagg cgacgcagag
     5821 ctccgtgacc gactgcaagc gtttcggtcc gcacaaaccg caagggtact ggaggcagag
     5881 ctatgagcgc gattcttccg ggggccacga tcggtatcat cggtggcggc caactggctc
     5941 gcatgatggt cctcgaggct agacgtatgg gataccgggt ctgcgtgctc tcgccggacc
     6001 catcggcgcc agcggtctcc ctcgcggatt cttggattcg gggaaacatc gacgatgtcg
     6061 ccgctggcga gcgctttgct cactcggtcg acgtcatcac cgtggacaca gagcatgtac
     6121 cagcctcgct gctggcccag ctcgagcttc tccgacccgt gcgaccgtca ccccaggtcc
     6181 tgcgcaccgt gcaagacaga catgcgcaaa gactcttcct cgatgccgtt gatgcgcccc
     6241 aaccggtttg cgtcgcggtc gattcacttc ccgagctatt ccatgaggca gacagagtcg
     6301 gcttcccgtg cgtgctcaag agccggcacg ccggctacga cggaaagggg cagaccgtag
     6361 ttcgtgagcg aggggggctc gcgagcgcat gggcaaatgt cggctccacc ggggcaatgc
     6421 tcgaggagct gatcgatttc gacttcgaga tttccgtgct gttggcgaga aatcccggag
     6481 gagaggttcg cttctacccg attgcccaca acgttcatcg tcatcacgtc cttcacgcga
     6541 cgatggctcc cgcacgcatc gacacggctg tcgaacacga ggccaaacgg atcgcaaacc
     6601 ggatcgcaaa cgccctcgat cacgtcggaa tgatggcagt cgagatgttc gtggtcgacg
     6661 gaacccgctt gctcgtgaac gagattgcgc cgcggccgca caacagcggg cactatactt
     6721 tcggagcgtg cgtgacttcg cagttcgagc agcacatccg agctatcgcc gacctgccac
     6781 ttggcgaccc ttcgctgccg cgccccgcag tcatggtgaa tctcttcggc gacctctgga
     6841 cggatggcgt cccggattgg acgccggtcc tatccaagcc cgaggtgcgg ttgcatctct
     6901 acggaaaggc ggagcctcgt cccggtcgaa aaatgggaca cgtgctggtt ctcgatcccg
     6961 atccatatcg ggcgcttcgg acaggcgaag ccctactgga cggtttgcgc gccaggtgcg
     7021 ccgatggccc aaagtccgaa atcgagaaca gcacatgtgt gagctgatcg acgtcgacgc
     7081 ggaatgcgtt gccccgaccc ctccggtcga cgacgaaatg ggggagtgat gaagatcagc
     7141 gtagccatgg gcgatggcat cggtcctgag atcatgggca ccgtactgag agtcttcgag
     7201 aaggcttcgg taccgctcga gtacgagctc gtcgagatgg gcaaggaggt ggtgctttcc
     7261 ggcgagcaga ccgggatcag tgcctcggct cgccaatccg tcgagtcatc cggaatactc
     7321 ttcaaggggc cgatggaaac cccgcagggc ggaggttaca agagcgtcaa cgtgacggcg
     7381 cgaaagctct ggggagcctt cgccaacaag cgcgtctatc ggtcctttcc aggcgtcccc
     7441 gcaccgatcg gcagccggca tctcgatctc acgatggtgc gcgagaacat cgaagacacc
     7501 tatggagcgg tcgagcacat gcagacccac gatgtcgcgc agtgccgccg gttcatcaca
     7561 cggccaggga gcgagcaggt gcatcggtac accttcgaga tggctgcccg aaagggcgct
     7621 cgccacgtga cctgtgcgca caaggcgaac atcatgaagc tcaccgatgg gctcttcctc
     7681 gaagtgttct acgacgtggc caaagattac cctgagatcg aggccgacga cgtcatcgtc
     7741 gatgcccttg ccatgcgcct ggtcctcgac ccctccgagt tcgaagtgat cgtcctgccc
     7801 aacctgcaag gagacattct cacggaccta gcggctgggt tggtgggcgg gcttgcgtac
     7861 gccccctcag caaacatcgg ggacggcatc tgcatcttcg aagcggttca tggaacggct
     7921 ccggatatgg tcggccagga cctggcgaac cccagtgcgc tcgttctgag cggaaccatg
     7981 atgctccggc atctcggact ggtccctcac gccacggcca tcgagaaggc gctcgaacag
     8041 acgctgcggt cgatgcacaa gcgacccgac ctcggtcagc ccatccccgt gttccgcacc
     8101 tcggtcttcg aggaaaagat gctcgaccaa ctcgacgccg tgcacattcc cgaaggagaa
     8161 ctgccccgca tggaggtcct acggaggacg gaacccaaaa tgcgcatcag ccctacgccg
     8221 gaacacacgg ttcttcgcgg cgtcgacgta ttcttggagt ccgggctgcc cccgaagctc
     8281 gtggaggcgc agctcaatga gatcggcgga tggctgaagc tcacgatgat ctcgaatcga
     8341 ggaacccagg tcgcgccgac cggatcggca tacacggact gcgtcaacca ctaccgcgct
     8401 cgcttcgaaa cgcagcagcc cgtatcgcag gagtcgatcg tgtcccagct cgcggccgtc
     8461 ggggagcgat tccggctctg cggggcggag tggcttcgcg agatcgacgg caagcagggc
     8521 tactccctcg cgcaggggca gagctgagct cgcacgcgct gccggtcttc gtcattgtgc
     8581 tatagcgccg tcggtggacc acctgatcga aaaagccgcg acgcttcagg aggcgctccc
     8641 gtacatccgg gagtttcatg gagacatctt cgtcatcaag tatggcggcc acgcgatggt
     8701 ggatccggcg cttcgcgaca gcttcgcgcg tgatgtcgtg ctcatgaagt atgttggctt
     8761 gcatcccgtc gtcgttcatg gcggcggccc gcagatcgac cagatgctca agtcgcttgg
     8821 cgtggtctcg gaacgactcg acggcctccg ggtgaccgac gatcacacga tggaagtcgt
     8881 cgaaatggtc ctgggcggaa agctcaatca ggagatcgtg tccctgattg caggtcatgg
     8941 cggccgcgcg gttgggctca cgggcaagga cgacgcgttt ctgcgcgcga cgaaggtcga
     9001 caagatgcaa accaagagcg gcaaatgggt cgatccgggc cgcgtcgggg tcgtcagtca
     9061 agtgaaccca gaggtcttgc gccggctggt cgatgatggc ttcattccgg tgatcgcacc
     9121 ggtcgcggtg gacgctcaag gcaagtctct caacgtgaac gccgacacgg ttgcgggcaa
     9181 gatcgcggag gcgctccctg cgcggaaatt cgttctgctc accgacatcg aaggcgttcg
     9241 ggggaaggat ggcgaggtcg ccagctcgtt gagcgccgct cagatcgacg ccctcaaggc
     9301 cgacggcatc atcgaaggcg gcatgattcc aaaggtcgac tgcgccctga gcgctctcgc
     9361 aggcggcgtc cgaaaggttc acatcatcga tggccgtgtt cgccatgcgg tgctgctcga
     9421 gatcttcacc gacgagggca tcggcaccga gatcacgcgc tgaacgagct gcgcaagggt
     9481 cgacggccct cggcaatggt ggtatgtcgc gggcttcccc caaggcacac catgaccagg
     9541 caggatgaca tgcttcgcac cgcgaaagag gttcagctcg gcaactacgc gcccgcgccg
     9601 ttcgttctga cgcacgggcg cggccgtcgc gtgaccgatg cctcggggcg cgagtatctg
     9661 gatctgtcgg gtgggatttc cgtgctctcc gtggggcaca gccacccaac gctcgccgcg
     9721 gcgatcggcg aacaggccgc gcgcctgatg cacgtctcga acgttttcta caacgatcgg
     9781 gccatcgagc tcgcagaagc gatc
//
LOCUS       DASRZF010000329         3606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1282347, whole genome shotgun sequence.
ACCESSION   DASRZF010000329 DASRZF010000000
VERSION     DASRZF010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282347"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(449..967)
                     /locus_tag="VFG22_14560"
                     /pseudo
     CDS             complement(449..967)
                     /locus_tag="VFG22_14560"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(992..1366)
                     /locus_tag="VFG22_14565"
     CDS             complement(992..1366)
                     /locus_tag="VFG22_14565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417517.1"
                     /translation="MADAYKMYLSATKMKPGSRDNCQRFWRDVESHIGHLYIEEVDTS
                     LIDELRSRLSTRLGPESINNGVKIVRAVLRFMWKRGHLKSVPYIPTQSAPELAAQWYT
                     EEERDRLLTGMFEMYPQWYAFF"
     gene            complement(1762..>3606)
                     /locus_tag="VFG22_14570"
     CDS             complement(1762..>3606)
                     /locus_tag="VFG22_14570"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HET6417518.1"
                     /translation="SMAQLLPLESTVYDLRDPLPDVGAMGSNGYGIGSDGSQTEYGLQ
                     LGNPHFPWRGSLRFYMQHLTIPGELDIMGATITGLPVVVIGFNQNIAWSHTVSTANRF
                     AIYEVEIVEGEPLKYRYDDEERDIEAVPVTIEVLLEDGTTEERTDNIYFTHYGPVIDG
                     GVLNPLLGGWPNPFDTLLTYRDANLTNNRAISYWDAMDKSQSIDDLEEASKIIGNPWT
                     NTIAADRNGEAFYGDISVVPNISTEKLDLCNDSFITMQINDFGYPALNGSESACEWGS
                     DADAPEEGIFGYGNLPKLRNREYVGNSNDSYWLSNPDQLLTGFSRVIGREEYPQSLRT
                     RQGFTQAEARMAATDGKSDTPGYNLALLQDMLYSDMDIAEQMTRTDVVALCDEVLDWS
                     GGDCNPDEDGAQPYSQNPNVAATACGILRDWDGLYQLDSVGSSLWREFWARVRGAANL
                     WAVPFDATDPVNTPNTLNVDDDGVVESVLCSIGGAVDRLVTAGVALDLPWGEAQYRPT
                     GDGVGRTPIHGGNGQSTFSVIGSRLVDGEGYSDIPAGNSYIHTVTWDETQCPDAFAVI
                     TYSQSSDPASPHYSDMTQLYSEGGWNDMPFCPEDIEATKISEIDVSTN"
BASE COUNT          599 a         1063 c         1163 g          781 t
ORIGIN      
        1 aacccggcac ggtgccgggt tttggctgcg ctttgccgtg actgacggcg cccttcggcc
       61 cctcgctgaa tcgattcgtc tgcgacatcg gcggggtttg tgatcgacaa gcgcggtgcg
      121 ctggccgcga tcaagcccgc ccggtgaccc aacgacgcaa agccaaatga tagccagatt
      181 tggtcagttt ctctcttcgt ttgtgttttg ttgtcgcgcc tcggtgccgt gagggtgccg
      241 tggcgatctt tgatcatgtg aaatcgagtg acgtgacgtg aacatggcga cgcgtgtact
      301 cgtgcatgat cattagggaa gcattgtttt cattgacgct ttacgagccg gcgaaacgga
      361 ccattggggt ccgtgccggg tcgccccttt tcttaatggg gctccggcga ttgggatgtg
      421 attctaggcg cttgcgggtt tgccgctgct accggttctc gttcgttgac ctgacgttga
      481 cgtgaggcgg cgaggcaggc cgcagggcct ccacgatgcg gagctgagct cgctgcccaa
      541 ggtgggcgta ttgatgcgtg cttctggccg acgtgtgccc gagctgggcc tgaatcgcct
      601 tgatcgagtg gccgccggtg gcggcctggc tggccaccga atggcggccc acccggtggt
      661 gaccggtttc ccgcaggccg agggctcgct gcacggcccg caggggcagt gcatgggagt
      721 agatgcgcgt ggggaagcac ccgtccttgc tgaacaggaa ccccgggccg gcgtactctt
      781 cccggatgtg ccagcgaatg gggtcgacca cgtcctcggt gagggcaagg gtcaacacct
      841 cgttgttctt gcgcggcccg agcgtggcag ggcgctcctt gttcccacgc tgcaccgatc
      901 ggtcgacgat gagctgcggg ggaatgtccc ggattcggtc cctcgagatg gcgtagactt
      961 cgcccgtgcg aagaccgagc cgcgtggtga gctagaagaa cgcgtaccac tgcgggtaca
     1021 tctcgaacat gcctgtgagc agcctgtcgc gctcctcttc ggtgtaccac tgagcggcta
     1081 gttcgggtgc gctctgcgtc ggaatgtatg gcaccgattt gaggtgccct cgtttccaca
     1141 tgaaccggag aacagcccgc acgatcttga cgccgttgtt gatggactcc ggtccgaggc
     1201 gcgtcgacaa cctgctcctg agctcgtcga tcaaagaggt atcgacttct tcgatgtaca
     1261 ggtgcccgat atgcgactct acgtcgcgcc agaagcgctg gcagttgtct cggctgccag
     1321 gcttcatctt ggtcgcggac aggtacattt tgtacgcgtc cgccatgcgg aagcgctttt
     1381 gccccaagat gagctcttcg cgctttcgtc ttccgcaatg aggtagcgct tgaagtgggc
     1441 gaccatgtgg aggccctggc gccgttgcgc ctccgcgctg agaccgggct ctctcagccc
     1501 attgggcgca ttcgatgaac cgatgcgctg taccaaatgt catgtcgcgt gcggcccgaa
     1561 gcgaaaaggg cgcgccggcg tcctgctcga gtggcgttcc agcgtcaggc tcttgagccg
     1621 ttcacacgga ggcgctcccg agaatcgtga ccactaggta ggatcagtct tcgtcgccgg
     1681 ccgtatccat cgcgcaatca aacgaaaacg gggccgaccc cgcggagagc cgaccccatc
     1741 ttcagcgtgt gtcgtgacag cctagttcgt gctgacgtcg atctccgaga tcttggtcgc
     1801 ctcgatgtcc tcggggcaga acggcatgtc gttccagccg ccctccgagt agagctgggt
     1861 catgtccgag tagtgcgggg acgccgggtc cgacgactgc gagtaggtga tgacggcaaa
     1921 cgcgtcaggg cactgggtct catcccaggt gaccgtgtga atgtagctgt tgcccgcggg
     1981 gatgtcggag tatccctcgc cgtccaccaa tcgtgaaccg atcacactga acgtcgattg
     2041 cccgttgccg ccgtggatcg gagttctccc cacgccatct ccagtaggac ggtactgggc
     2101 ctctccccag ggaaggtcga gcgcaacgcc cgcggtaacg agccgatcga cagcgccacc
     2161 gattgaacag agcacgctct cgacgactcc atcatcgtcg acgttgagcg tattgggcgt
     2221 gttgacggga tccgttgcgt cgaagggaac ggcccaaaga ttggctgcgc cgcgcacgcg
     2281 ggcccagaac tctctccaga gcgaagagcc cacgctgtcg agttggtaga gcccgtccca
     2341 gtcccgaagg atgccacatg ccgtggcggc gacgttcggg ttttggctgt agggctgggc
     2401 gccgtcctcg tctgggttgc agtctccgcc actccagtcg aggacctcgt cgcagagcgc
     2461 gacgacgtcc gtgcgagtca tctgctctgc gatatccatg tcgctgtaga gcatgtcttg
     2521 aaggagcgcg aggttatacc ccggcgtgtc gctcttgccg tccgtcgcgg ccatgcgtgc
     2581 ttcggcctgc gtgaagccct gcctcgtacg aagcgattgg gggtattcct cgcgtccgat
     2641 gacccgcgag aaacccgtta gcagttggtc ggggttgctc agccagtagc tgtcgttgga
     2701 gttgccgaca tactctcggt ttcggagctt cggtagattc ccgtagccga aaattccttc
     2761 ctccggtgcg tctgcgtcgc ttccccactc gcaagcactc tcggacccat tgagggccgg
     2821 gtagccgaaa tcgttgatct gcatggtgat gaagctgtcg ttgcacagat cgagcttctc
     2881 cgtcgagatg ttcggtacga ccgaaatgtc accgtagaac gcctcaccgt tgcggtcggc
     2941 ggcaatcgtg ttcgtccaag gattaccgat gattttggat gcttcttcca gatcgtcgat
     3001 cgattgggac ttgtccatcg cgtcccagta gctgatcgcc cggttgttgg tgaggttggc
     3061 gtctcggtac gtgaggagcg tgtcgaaggg gttcggccag ccgccgagga gaggattgag
     3121 cacaccgccg tcgatgaccg gcccgtagtg cgtgaagtag atgttgtcgg ttctttcctc
     3181 ggtcgtcccg tcctctagca ggacttcgat agtgaccggt accgcctcga tgtcgcgctc
     3241 ttcatcatcg tagcggtact tcagcggctc cccctctacg atctcgacct cgtatattgc
     3301 gaagcggttc gcggtcgaca cggtgtgtga ccacgcaatg ttctgattga agccgatgac
     3361 gacgacggga agtccggtaa tcgtcgcgcc catgatgtcg agctcgccgg ggatcgttag
     3421 gtgctgcatg tagaaccgaa gactgccgcg ccaaggaaag tgcggattcc ctagttgcag
     3481 gccgtactcg gtttgggagc cgtcggagcc aatgccgtac ccattgcttc ccatcgcgcc
     3541 gacgtccggt agcggatcgc ggaggtcgta gacggtgctc tcgagtggga ggagctgtgc
     3601 catcga
//
LOCUS       DASRZF010000330         2137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1980742, whole genome shotgun sequence.
ACCESSION   DASRZF010000330 DASRZF010000000
VERSION     DASRZF010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1980742"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="VFG22_14575"
     CDS             complement(<1..939)
                     /locus_tag="VFG22_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417519.1"
                     /translation="MDADVISIGSEAEGTLSLVQIADLIESLHGGDCKHPRDLKALFG
                     IPDEDWERVQRELGPAHRAAQRRESGVDARHRDLNRALRDLALAFPPYSTESDLDLIQ
                     KLVGPPLRQRELESAMQDLVLRPATRARVLVGRFEKLAPFDQFAYVIDSAYFAYCRGN
                     VVASLMCVLPVIEGVIVRWAEDVGGTLETGFGGYAEWVESTPTRAPLLGNPAFADSYA
                     ETCGHILSEHLYKKTWEGPAFGLFNRHVALHLKEERGFATPQNLVRAFLLLDLMSELL
                     ICERWDRDPMFHVTSEEEAPLCSAYQQAATSQLTPPT"
     gene            complement(1091..1468)
                     /locus_tag="VFG22_14580"
     CDS             complement(1091..1468)
                     /locus_tag="VFG22_14580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417520.1"
                     /translation="MLTSKAPSLLAVLLLLALASCREPTALSNEYSSLAGTWRAGSNC
                     FALDRSGTTSYALEGDTSSQYLRGGQLYSIGDGYIEVGLPRAVWPDRVEVTTTPYLSS
                     SGLAIRVAGVEAYRVTDSGPCGI"
     gene            complement(1878..>2137)
                     /locus_tag="VFG22_14585"
     CDS             complement(1878..>2137)
                     /locus_tag="VFG22_14585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417521.1"
                     /translation="STGADEDAMKLSALTDFVDRLDAAEIHYTLSSIRAGAILVEVSI
                     EDERWEVEFMTDGDVEVEIFTSNGDIHDASILEDLFERDSD"
BASE COUNT          396 a          632 c          693 g          416 t
ORIGIN      
        1 tgtgggtggc gtgagctgac tggtcgctgc ttgctgatag gcactgcaaa gcggcgcttc
       61 ctcttccgaa gtcacgtgaa acattggatc tcgatcccag cgctcgcaga tgagtagctc
      121 cgacatgagg tccaagagta ggaatgcccg cactaggttc tgtggagtag caaagcctcg
      181 ctcctccttg aggtgtaagg cgacgtgacg attgaagagt ccgaaggcag gcccttccca
      241 cgtcttcttg tagaggtgct ccgaaagaat gtgcccgcat gtctccgcgt acgaatcggc
      301 aaacgccgga ttccctaaca atggtgccct agttggagtc gactcaaccc attcggcata
      361 gccaccgaac cccgtttcta gggtgccgcc gacatcttcc gcccatcgca cgatcacgcc
      421 ttcgatcact ggcaagacac acatgaggga tgcaacgacg ttccctcgac agtatgcgaa
      481 gtaggcggag tcgatgacgt aggcgaattg atcgaaggga gcgagctttt cgaaacgacc
      541 cacaagaacg cgagctcgag tggcaggtcg gagaactaag tcctgcatcg cgctctcaag
      601 ctcgcgctgt ctcaggggcg gacctactaa cttttggatc agatcgaggt cactctcagt
      661 cgagtacggc ggaaatgcga gcgcgaggtc acgcagagct ctgttgaggt ctcggtgcct
      721 tgcatcgact ccggactcac gcctttgagc ggcacgatga gctggaccca gctcgcgctg
      781 cactctttcc cagtcttcgt cggggatgcc gaagagcgcc ttcagatccc tggggtgctt
      841 gcagtcgccg ccgtgcagac tctcgatgag gtcggctatc tgaaccaagc tcaaggttcc
      901 ttctgcttcg ctcccgatcg agattacgtc tgcatccatg ctcaggtcgt ataacggatc
      961 ggcgttcagc ggcgtgcgcc tacctggcac agcgagcgtg gacgcctcgg ttcacgcgat
     1021 tctatcacga cccatatcgg cacagcgtga gcaagtccgc tgcaacggat tgttaggcgg
     1081 cggctcgcgc tcagatcccg cacgggccgc tgtcggtgac gcgatacgcc tcgactcctg
     1141 caacccggat cgccagaccc gagctcgaga ggtacggggt ggtggtgacc tcgacacgat
     1201 ctggccagac ggctcgcgga agcccgacct cgatgtagcc gtctccgatc gagtagagct
     1261 gaccacctct gaggtactgc gaggaagtgt cgccttcgag tgcgtagctc gtggtgccag
     1321 agcgatcgag tgcgaagcag ttggatccgg ctcgccaggt ccctgcgaga gacgagtact
     1381 cgttggaaag tgccgtgggt tcacgacaac tcgcaagcgc gaggaggagg agcaccgcga
     1441 gaagcgaggg agctttcgac gtaagcattc actttgctcg cgaggctggc gacgcggccg
     1501 cctaactact tatttgcccg agacaccgat cgtcttggag tgacccgatc gtcgcataat
     1561 tggcactggg ctggaaggaa ggtggcgtcg tcgtggggga atctgggtgc gccgtgttgt
     1621 tttgcggcgt tgcggagcgg tgctggagac gccgtgaagt tccacagcgg gggcgccggg
     1681 gctgggccgg caatggcggg cgcgtccccg cgccggttct gggttgaagc aaccggaccc
     1741 gatctgcccg gccggacgtg ccccgcgggc cgggcgggac aggcccagcg cggggcgggg
     1801 agcgttgacc gcgcggacac ctcgggtgtc gcgtccccca ccgcgccgct ttgttgggcg
     1861 cgtacccggc cgagggttca atccgagtcg cgctcgaaga gatcttcgag gatcgacgcg
     1921 tcgtggatgt cgccgttgct cgtgaagatc tcgacttcga cgtcgccgtc ggtcatgaac
     1981 tcgacctccc agcgttcgtc ctcgatcgag acctcgacga ggatcgcgcc cgctcgaatg
     2041 ctcgaaaggg tgtagtggat ctcggctgcg tcgaggcgat ccacgaaatc ggtgagtgcg
     2101 gatagcttca tggcatcctc gtcggcgcct gtcgacg
//
LOCUS       DASRZF010000331        12074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_5067737, whole genome shotgun sequence.
ACCESSION   DASRZF010000331 DASRZF010000000
VERSION     DASRZF010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 12074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12074
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5067737"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="VFG22_14590"
     CDS             complement(<1..766)
                     /locus_tag="VFG22_14590"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HET6417522.1"
                     /translation="MFDLSNTFYGNEIRSWLIALGIGIGTLLVLHVLEQVLVVRIERL
                     TRKTHTIIDDLVVGALRKTKLLFLFVVSSFIGTLSLQLPAEVRDILWKVTIVAAIVQA
                     GIWISTALTIWLENYRKGEEDGGNRMTMNALSFLGRIVLWATALLLILDNLGVDVTAL
                     VAGLGIGGVAIALAVQNILGDLFASLSIVLDQPFVLGDFIVVGDLAGSVEHIGIKTTR
                     IRSISGEQLVFSNADLLGSRIRNFGRMERRRVVFSIG"
     gene            complement(768..1382)
                     /locus_tag="VFG22_14595"
     CDS             complement(768..1382)
                     /locus_tag="VFG22_14595"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HET6417523.1"
                     /translation="MSQLPKGLVRGEDGLARCWWAAKTPDYAAYHDEEWGWPVHDDQR
                     LFEKICLEGFQSGLSWLTILRKRDAFRRAFSDFDYHRVARFNQRSVARLLGDASIVRH
                     RGKIESTINNAKRALELEEEFGSLAAYFWEYVPSNGERPRRMTQAALRKLSETPTSTM
                     LSKDLKRRGWSFVGPTTAYAFMQAMGLVNDHLETCFVRERIART"
     gene            complement(1438..1629)
                     /locus_tag="VFG22_14600"
     CDS             complement(1438..1629)
                     /locus_tag="VFG22_14600"
                     /inference="COORDINATES: protein motif:HMM:NF024851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HET6417524.1"
                     /translation="MKILFDRSKCQLHAQCRGAAPELFSFAPDGTLVILDENPPEELR
                     EALQDAVDACPEGAIAIGD"
     gene            complement(1641..2843)
                     /locus_tag="VFG22_14605"
     CDS             complement(1641..2843)
                     /locus_tag="VFG22_14605"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET6417525.1"
                     /translation="MENKCPAKFSNVDFCAPETHDNPYEMYDYFLEHEPIYFDEKNGV
                     WEVFRYDDIVAIERDTETFINGEGVRPNIPPDPGMIYQDGEQHTKQRALVASGFTPRQ
                     MRKMEDRAREICVDLVDKMLAKDEVDIVEDLAGALPARLIADMLDIPEEKRAAVMSWI
                     NAITAGGCGPDYVTDEVNEAFENFCMHHEEMYQDRIASGCTGADLLTIWINAELNGEK
                     LDESQLLFEHVLLNVGGAETTRNAIAGGLQQLSEDQKQYDAISEDPSLIPNAVEEIIR
                     WVCPFVNMYRTATRDYELHGKVIKEGQQVGLTYPAANRDPRKFEDPHRFNIFRDFSKE
                     AKHLSFGLGPHFCLGASLARLEVRLSLEEMTQRVKRVRIPEGRKVEYVSSSFTRGLKK
                     FPAVLEPR"
     gene            complement(3027..3899)
                     /locus_tag="VFG22_14610"
     CDS             complement(3027..3899)
                     /locus_tag="VFG22_14610"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6417526.1"
                     /translation="MTEIANDNLGFVECWNEILTPKWIRFRHLLSGNGKIHSDIAYSD
                     YGIRKGDKVLDIGCGFGETALEIGEMVGPGGEVVGIDCTDAFVDIANQERDAAGAKHV
                     RYAVADAQICDLPEGYFDVVYSRFGVMFFQSAVRALRNANRALKPGGKVCLIVWRSLA
                     DNPAWGAAKEVVLRYLPPPGDAASTCGPGPFSMAGEDTDRAMLAAAGFSEVEVFRRID
                     ADICIGRDIDEAIDYQILVGPSGEIVREAGEEGQRKLPEIRRELHALMQPYLREGGVF
                     MPSSTWSIMARKPL"
     gene            complement(3952..4617)
                     /locus_tag="VFG22_14615"
     CDS             complement(3952..4617)
                     /locus_tag="VFG22_14615"
                     /inference="COORDINATES: protein motif:HMM:NF016705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HET6417527.1"
                     /translation="MPKRIWIKLIATVLLLGAVGAFIAVLRGGKMAYESPRYEIVERM
                     DGLEVREYEPYLVAETRVQGTLETAGNQGFRILAAYIFGNNQGKQRIAMTSPVSQEGA
                     EGSKIAMTAPVTQERVGAEFVIRFMMPSELSLSTLPTPNDPRIEIREIPARRLAAIRY
                     SGRWTKERYEVYLTKLRETLRRNGYEPVGEPLWARYNPPFMPWFLRRNEILTDFAPIP
                     KSG"
     gene            complement(4650..5027)
                     /locus_tag="VFG22_14620"
     CDS             complement(4650..5027)
                     /locus_tag="VFG22_14620"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HET6417528.1"
                     /translation="MIVVTNRIPVAEGYEIDFEDRFRKRVHLVDQAKGFIRNEVHRPR
                     PMELDHQTGEWTRGPVGSGYYEVKTWWESFDDFVAWTTSPSFAEAHRNRPPKEMFRGP
                     NELTIHEVFLSTDGTSTSEAQAD"
     gene            5083..6432
                     /locus_tag="VFG22_14625"
     CDS             5083..6432
                     /locus_tag="VFG22_14625"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET6417529.1"
                     /translation="MDLEKPRIVIVGGGFAGVAAARALRRAKANVVLIDKHNHHLFQP
                     LLYQVATAALHPGTIAAPIRSLVSSQPNCRVLMKEVEDIDVESRTLTLGGEPYTYDYL
                     VLATGYATNYFGNDQWKTVAPGLKTIDEATDVRARFLLAFEQAEFELDKAAQRAALTF
                     AIVGAGPTGVEMAGALAGIADSVRKDFRSIDTRTARIILIDFADRVLTSFHPELSERA
                     KRDLESLGVEILLETRVTDVDERGLTAETRDGAVRIDANNVIWAAGVKGCPLAEALGA
                     ELDRGGRVLVEADMSIPGHPEVFVAGDLAHLTDPKTAQPVPAVAQGAIQGGKFVGQTI
                     AAEVNALAAGEPPPPRKAFQYRDKGSMAIIGRNRAVAEIGSLRFGGYFAFVLWAMIHI
                     LFLIDFRRKLVVFVEWTWLYFTGGRGARLITGDDRMPKTIQPPPDFRLIARDSQPHE"
     gene            6471..8129
                     /gene="gpmI"
                     /locus_tag="VFG22_14630"
     CDS             6471..8129
                     /gene="gpmI"
                     /locus_tag="VFG22_14630"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HET6417530.1"
                     /translation="MTDWKLKKHRTFPGVRGPVLIVVMDGVGCGASDEGDAVWLARTP
                     TLDRLAGVSLTTRLRAHGTAVGLPSDDDMGNSEVGHNAFGAGRVFDQGAKRVQMAIDS
                     GEMFEGEEWKKLVRRVNESGEPMHFIGLLSDGNVHSHIEHLFAMLRRCNDEGVQHARV
                     HILLDGRDVPETSALEYVDALEKVLNELNEQGGRDYRIASGGGRMFITMDRYNADWSM
                     VERGWKLHVRGEGRGFASATEAIQTIREENPGMIDQDLLGFVIVDSEGRAVGPIRDGA
                     AVVLFNFRGDRALELTRAFTEPELSKFERGPIPDVEFVGMMEYDGDLKLPRHYLVQPP
                     KIDRTMGEYLARNQVSQFACSETQKFGHVTYFWNGNRTGMFDEVYEEYVEVPSDVVPF
                     EQRPWMKAAEITDATIKALTGGGVRHARINYANGDMVGHTGNLDASVIAVEAVDLSLG
                     RIIPVIEKLGGALIVTADHGNCDEMFEVDKKSGAFKRDAAGHLRSKTSHTLNAVPCYL
                     YVPGHETLRLDESIAHPAITNISATTFQLLGYERPEGYDSSLLE"
     gene            complement(8146..8814)
                     /locus_tag="VFG22_14635"
     CDS             complement(8146..8814)
                     /locus_tag="VFG22_14635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417531.1"
                     /translation="MTSKKKTKEEETDAVAATPAKKSAGGGAGAAARKILSDESPQSK
                     IAEHGETMMEGPRTAATQSARVLSEVISEKPELVVSLVAKFAKGVSSSNKRVVQTSAE
                     ALPAIARIAPARVARQLEVLKTSFATANDVGKDGLVKTFAALCTASVAYQKRLEPVLT
                     LALKGADGKTLLAWTQIVLPALKGEPHARARAVVEGRLDLIPRSYAQQIADFLGIKLR
                     IRYR"
     gene            complement(8811..9824)
                     /locus_tag="VFG22_14640"
     CDS             complement(8811..9824)
                     /locus_tag="VFG22_14640"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HET6417532.1"
                     /translation="MRSPPMKGRVVRTSHESQCLRDNPLGDPHRRDLYVYLPPQYDES
                     KRFPVVMLLAGFGATNHSIAAWSPWKPNTLELFDRLVAEGRCQPAVLVLPDCFNRWGG
                     SQFIDSEGTGRYQTYLADEVFAFVDAEFRTIPDRLARAVVGRSSGGFGALRLGMDRPD
                     VVSVIGSHAGDAAFEVSMRPMLTSAAIAIDLAGGLHAFAEEIPASGPKNAKEFDAIFV
                     LAAGAAYSPDPGAFPFAQIPVDPSTGQIDEAVWARWLEQDPLGRIEASLAALQRMSII
                     YVDAGNRDEHGLHFAARLLDEALRKHGLPVRYEEYEGGHRGTSWRYEASLPLIVEALQ
                     GPE"
     gene            complement(9867..11627)
                     /gene="argS"
                     /locus_tag="VFG22_14645"
     CDS             complement(9867..11627)
                     /gene="argS"
                     /locus_tag="VFG22_14645"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HET6417533.1"
                     /translation="MLLERYLESLGSQAIQDALQLDGPRPAIVRPTQDPKFGDFQING
                     AMPLGKELGKPPRELAQPIAEALSRIDAIEKAEVAGPGFVNIHLAPAWVASKLGEALT
                     DASRDGVPVVEPAEKIIVDFSSPNIAKQMHVGHLRSTIIGDAIARILSFMGHDVVRDN
                     HLGDWGTQFGLLIVGMREWGEESALLSDPIKELERVYKLASERAGNEEVFADRARAEL
                     AKLQNGDPENRALWEHFVEVTRGALETVYAELDVSFDVWLGESAYHDALPKVVDELLE
                     RGLAREDDGAVCIFWDEIDGAPKALRKQKTPFIVRKKDGAFLYSTTDIATVEYRRDHF
                     QADRALYVVDNRQGLHFQQLFTIMKLLGVEMELEHVGFGTVLGDDGKPLRTRDASGAV
                     ITLASLLEESKERARQRIDEGIAAGRLRVRPEEMDEVSKVVGIGAVKYADLRQNRLSD
                     YQFDWDKMISFQGNAGPYLQYAYARCASIFAKGGIEMDSVAVGTTIALDAAAEQTLGK
                     HLLRFSDVVYQAGATSQPHLICEHVYELARAFNGFYAECPVLDAEGDVRESRLGLTAL
                     TARQIRRGLGLIGIGVLDRM"
     gene            11663..>12074
                     /locus_tag="VFG22_14650"
     CDS             11663..>12074
                     /locus_tag="VFG22_14650"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6417534.1"
                     /translation="MNVIRAMMGGLRPVIRARRAIFGPRRPTWTLELETVAEVLRRSA
                     NLSTVLPLQIQRRVIDPPRPVTKPMREVDMKPVDAGGVSAEWFSQPASDRSRVILYLH
                     GGGYSTGSINSHRDFLARLCIASGMRVLAADYRLA"
BASE COUNT         2244 a         3790 c         3687 g         2353 t
ORIGIN      
        1 ccccgatcga aaacaccacg cgtcgccgtt ccattcgccc gaagttgcgg atccggctac
       61 ccagaaggtc ggcgttggaa aacacgagct gctcacccga gatgctccga atccgggtgg
      121 tcttgatgcc gatgtgctcc acggagccgg ccaggtcccc gacgacgatg aaatcaccca
      181 gtacgaatgg ctggtccagg acgatcgaga gcgaggcaaa gaggtcgccg aggatgttct
      241 gcaccgcgag cgcgatcgcc acgccaccga tgccaaggcc tgccaccagg gcggtcacgt
      301 cgaccccgag gttgtcgaga atcagcagca gcgccgtggc ccagagcacg attcggccga
      361 ggaagctcag ggcgttcatc gtcatccgat tgccgccgtc ctcctccccc ttgcggtagt
      421 tctcgagcca gatcgtgagc gcggtgctga tccagatccc ggcctggacg atcgccgcca
      481 cgatcgtgac cttccagaga atgtcgcgca cctctgctgg cagctgcagc gagagcgtgc
      541 cgatgaacga tgacaccacg aatagaaaca agagcttggt cttgcggagc gcgcctacga
      601 ccaggtcgtc gatgatggtg tgcgtcttgc gggtgagccg ctcgatccga acgaccaaga
      661 cctgctccag cacatgaagg acgagcaggg ttcctatgcc gatccctagc gcgatgagcc
      721 agctgcgaat ttcattgccg tagaacgtat tgctgaggtc aaacactcta ggttcttgcg
      781 atgcgttcgc gaacgaagca agtctctaaa tggtcgttga ccagccccat cgcttgcatg
      841 aacgcatagg cggtggtcgg accgacgaaa gaccagccac ggcgcttgag gtccttgctc
      901 agcattgtgg aagtgggcgt ctccgaaagc ttcctcagcg cagcctgcgt catccgtcgg
      961 gggcgctccc cgtttgacgg cacgtattcc cagaagtacg cggcaagcga gccgaactcc
     1021 tcctcgagct cgagagcccg tttggcattg ttgatggtgg attcgatctt gccccggtgc
     1081 cgaacgatgc tcgcgtcccc gagaagccgc gccacgctcc gctggttgaa gcgcgccacg
     1141 cggtgatagt cgaagtccga aaaggccctc cggaatgcgt cacgctttcg caggatcgtg
     1201 agccagctca gcccggactg aaaaccctcc aggcagatct tctcgaacag gcgctggtcg
     1261 tcgtggaccg gccatcccca ctcttcgtcg tgatacgcgg cgtaatcggg ggtcttcgca
     1321 gcccaccagc atcgggccag cccgtcctcg cctcggacga gccctttcgg cagttgagac
     1381 atcggcgcca aacctaccat gcaagcggcg aggctcgagc gggttcgaag ccgaagctca
     1441 gtccccgatg gcgatggcgc cctctgggca ggcgtcgacc gcgtcttgaa gggcttcgcg
     1501 gagctcctcg ggtgggttct cgtccaagat cacgagggtc ccatcggggg cgaacgaaaa
     1561 gagctcgggc gcggcaccac ggcattgggc gtgcagctgg cacttacttc ggtcgaacaa
     1621 gatcttcatg attcacccca tcatcgcggt tcgagcaccg cagggaactt cttgaggccc
     1681 cgcgtgaacg aggaggacac gtattcgacc ttcctgccct ctgggatccg aacgcgcttc
     1741 acacgctgcg tcatctcctc gagtgaaagg cgcacctcga ggcgcgccag agaagcgcca
     1801 aggcagaagt gcggcccaag cccgaaggac aggtgcttcg cttccttcga gaaatcccgg
     1861 aagatgttga atcgatgcgg gtcctcgaat ttacgagggt cgcgattcgc agccgggtag
     1921 gtgagcccga cctgctggcc ttccttgatg acctttccgt gaagctcgta gtcccgggtt
     1981 gcggttcgat acatgttcac gaacgggcag acccagcgaa tgatctcttc gaccgcattg
     2041 gggatcagcg acgggtcctc gctgatagcg tcgtattgct tctggtcctc ggaaagctgc
     2101 tgaaggcctc cggcgattgc attgcgcgtg gtttcggccc cgcccacgtt gagtaggacg
     2161 tgctcgaaca ggagctgtga ctcgtcgagc ttctcgccgt tgagctcggc gttgatccag
     2221 atcgtgagca gatcggcccc tgtacagccc gacgcgatgc gatcctgata catctcttcg
     2281 tgatgcatgc agaagttctc gaatgcctcg ttcacctcat cggtgacgta gtcgggaccg
     2341 cagcctccag cagtgatcgc gttgatccag ctcatcaccg cagcgcgctt ctcttcgggg
     2401 atgtccagca tgtcggcgat cagacgggcg gggagcgccc cagcgaggtc ctccacgatg
     2461 tcgacctcgt ctttggcgag catcttgtcg acgaggtcta cgcagatctc ccgtgcacga
     2521 tcttccatct tccgcatctg gcgtggcgtg aatcccgaag ccacgagcgc cctctgtttc
     2581 gtgtgctgct cgccgtcttg gtagatcatg ccgggatccg gcggaatgtt tggccgtacg
     2641 ccctcgccgt tgatgaacgt ctccgtgtcg cgttcgatcg cgacgatgtc gtcgtagcga
     2701 aagacctccc aaacgccgtt cttctcgtcg aagtagatcg gttcgtgctc gaggaagtag
     2761 tcgtacattt cgtacgggtt gtcgtgcgtc tcgggggcgc agaaatcgac gttgctaaac
     2821 ttcgctgggc acttgttttc caaaggatcc tccgtatatc cgtcagacgg gatataactt
     2881 tactacaagt aaactaaccg tagtgtagaa caagttctac tcgggtcaag cgcaatgcag
     2941 cccattgacc gaaagatgac cgaacgactg cgcgacggca ggaggcgagc gcgccggagc
     3001 ttcgccattt cccacgtgcc gccgcttcag agcggcttgc gcgccatgat cgaccaggtg
     3061 ctcgagggca tgaagacgcc gccttcgcgc aggtagggtt gcatcagcgc gtgaagctcg
     3121 cgtcggatct ctggaagctt gcgctggcct tcctcgccgg cctcccgaac gatctcaccc
     3181 gagggtccca cgaggatctg gtaatcgatg gcttcatcga tgtcgcggcc gatgcaaatg
     3241 tcggcgtcga tacgtctgaa cacctcgact tccgaaaagc ctgctgccgc gagcatggcc
     3301 cgatcggtgt cttcaccagc catcgagaac gggcccggtc cgcaggtcga tgcggcatca
     3361 ccgggcggcg gcagataccg aagcacgacc tccttcgcgg caccccaggc cgggttgtcc
     3421 gccagcgagc gccagacgat cagacacacc ttgccccccg gcttgagcgc gcgattcgcg
     3481 tttcgaagcg cacgtaccgc gctctggaaa aacatgacgc cgaagcggga atacacgacg
     3541 tcgaaatacc cttccggaag atcgcaaatc tgcgcgtcgg cgactgcgta gcgcacgtgc
     3601 tttgcgccgg ctgcatcgcg ctcctgattg gcgatatcga cgaatgcgtc ggtgcagtcg
     3661 attccgacca cctctccccc aggcccgacc atctcgccta tttcgagagc ggtctcacca
     3721 aagccgcagc cgatgtcgag gactttgtcg ccttttcgga ttccgtagtc cgaataggcg
     3781 atgtcactgt gaatcttgcc gttccctgaa agcagatgcc ggaatcgaat ccacttggga
     3841 gtgaggatct cgttccagca ttcgacgaat cccaaattgt cgttcgcgat ctcggtcatt
     3901 ttcaccgccc agctcgattc tgcccttctg cggactcgat ctcaacgatc ttcatccgga
     3961 cttcggaatc ggggcgaagt cggtcagaat ctcgtttcgt cgcaaaaacc atggcatgaa
     4021 cggcggattg taccttgccc agagcggttc gccgaccggt tcgtagccgt tgcggcgaag
     4081 cgtctcccga agtttcgtca ggtaaacttc gtatcgttct ttggtccatc gcccggagta
     4141 gcgaatcgcg gccagccgac gcgccggtat ctcgcggatt tcgatgcgtg ggtcattcgg
     4201 agtcggcaag gtcgaaagtg agagctcgga tggcatcatg aatcgaatca cgaactcagc
     4261 gccgacacgc tcctgcgtaa ctggcgcggt catcgcgatc ttgcttccct ccgctccttc
     4321 ctggctgacc ggcgaggtca tcgcaattct ctgcttgccc tggttgttgc cgaaaatgta
     4381 cgcagccaag atccgaaacc cttggtttcc cgcggtctcc agggtcccct gcacgcgagt
     4441 ctccgcgacg aggtagggtt cgtattctcg cacctcgagg ccgtccatgc gttcgacgat
     4501 ctcgtatctc ggactttcgt aggccatttt gccacctcgt aagactgcga tgaacgcccc
     4561 cactgcgccg agtagaagca cagtcgcaat gagcttgatc cagatccgtt tcggcaccgg
     4621 gtccttatac accgcgagca tcgccttgct cagtcggcct gcgcctcgct cgtgctcgtt
     4681 ccgtcggtgc tgagaaacac ctcgtggatc gtgagctcgt tggggccccg gaacatttcc
     4741 tttggaggtc ggtttcggtg cgcttcggcg aacgaaggac tcgtggtcca ggcgacgaaa
     4801 tcgtcgaacg attcccacca agtcttgacc tcgtagtagc cgctgcccac cgggccccgc
     4861 gtccattcgc cggtttggtg gtctagctcc atcggtcgcg gccggtgcac ttcatttcga
     4921 atgaacccct ttgcctggtc cacgaggtga acgcgcttgc ggaaacggtc ttcgaaatcg
     4981 atttcgtagc cctcggctac ggggatgcgg tttgtcacga cgatcatggg ccaatcatac
     5041 cccgcttccg ctttggtgca gccggcgcta gagctgcagg gcatggacct cgaaaaacct
     5101 cgtatcgtga tcgtaggcgg cggatttgct ggagtggccg cggctcgagc ccttcggcgc
     5161 gccaaggcca atgtcgtcct catcgacaag cacaatcacc atctctttca gccccttctg
     5221 taccaggttg ccaccgctgc tctgcatcct ggcaccatcg cagcgcccat tcggagcctc
     5281 gtcagcagcc agccaaattg ccgcgtattg atgaaagagg tcgaagacat cgacgtcgag
     5341 tccaggaccc tcacgcttgg cggcgaaccc tacacgtacg actatctggt gctcgcaacg
     5401 ggctacgcga cgaactactt cggcaatgat cagtggaaga cggtggctcc cggtctgaag
     5461 acgatcgacg aggccacgga cgttcgcgcc cgcttcttgc tggcgttcga gcaagccgag
     5521 ttcgagctcg acaaggctgc gcaacgggca gcgctcacat tcgcaatcgt cggcgccggg
     5581 cccacaggcg tagaaatggc aggggcgctc gcgggcatcg cagactccgt gcgcaaggac
     5641 tttcgctcga tcgacacccg aaccgcgcgc atcatcctga tcgactttgc cgaccgggtt
     5701 ctgacctcgt ttcacccgga gctctcggag cgcgcgaagc gggacctcga gtctctcggc
     5761 gtcgagatct tgctggagac gcgcgtcacg gacgtcgacg aacgaggcct cactgcggaa
     5821 acccgagacg gtgcagtccg gatcgacgcg aacaacgtga tctgggccgc tggcgtcaag
     5881 ggctgcccat tggccgaggc gcttggcgcc gagctcgacc ggggagggcg tgtgctcgtg
     5941 gaggcggaca tgtcgatacc gggccacccc gaggtgttcg tcgccggtga cttggcacat
     6001 ctcaccgatc ccaagaccgc tcagccggtg cctgcagtgg cccagggtgc gattcaagga
     6061 gggaagttcg tcggccaaac catcgccgcg gaagtcaacg ccctcgctgc tggtgaaccg
     6121 ccgccgccgc gcaaggcgtt ccagtatcga gacaaaggtt cgatggcgat catcggccgc
     6181 aaccgcgccg tcgcggaaat cggtagtcta cgtttcggcg gctatttcgc gttcgtgctc
     6241 tgggcgatga tccacattct gttcttgatc gatttccgaa gaaagctggt cgtcttcgtc
     6301 gagtggacct ggctctactt cacgggaggc cgcggtgcgc ggctgatcac gggcgacgac
     6361 cgaatgccga aaacgatcca accgccacca gatttccgcc tcatcgcccg cgactcgcag
     6421 ccgcacgagt agctcgtcga agttcgagtg gactgtgcta ggaaaccccg gtgaccgatt
     6481 ggaagctcaa aaagcaccga acgttccctg gcgtacgcgg tccggtcctg atcgtcgtga
     6541 tggatggcgt cggctgcggc gcttcggacg agggcgacgc ggtgtggctt gcgcgaacgc
     6601 cgacgctcga tcggctcgcc ggcgtttctc tgactacgcg gctccgcgcg cacggaacag
     6661 ccgtcgggct tcctagcgac gacgacatgg ggaacagcga agtcgggcac aacgcgtttg
     6721 gagcgggccg cgtcttcgac cagggcgcca agcgcgtcca gatggcgatc gacagtggcg
     6781 agatgttcga gggcgaggag tggaagaagc tcgtccgccg tgtgaacgag agtggcgaac
     6841 cgatgcattt catcggattg ctctccgacg gcaacgttca cagccacatc gagcaccttt
     6901 tcgcgatgct ccggcgatgc aacgatgagg gcgttcagca tgcacgcgtg cacatcctgc
     6961 tcgatgggcg cgacgtcccg gaaaccagcg ccctcgaata cgtcgacgcg ctcgaaaagg
     7021 ttttaaacga gttgaacgag cagggcggac gcgactatcg aatcgcgtcg ggtggcggcc
     7081 gtatgttcat cacgatggac cggtacaacg ccgactggtc gatggtcgag cgcggctgga
     7141 aactccacgt gcgcggcgag ggtcgcggct ttgcgagcgc cacagaagcg attcagacga
     7201 tccgggagga gaatcccggc atgatcgatc aggatctgct cggcttcgtc atcgtcgatt
     7261 ccgaaggccg ggcagtcggc ccgattcgcg atggcgccgc cgtcgtgctc ttcaattttc
     7321 gtggcgaccg agcgctggag ctcacccggg cgttcaccga gccggagctg agcaagttcg
     7381 aacggggtcc gattccggat gtcgagttcg tcggcatgat ggagtacgac ggcgacctca
     7441 agctgccaag gcactacctc gttcagccgc cgaagatcga ccgcacgatg ggcgagtacc
     7501 tcgctcgtaa tcaggtcagc cagtttgcgt gcagcgagac gcagaagttc ggtcacgtca
     7561 cctatttctg gaatggcaat cgcaccggaa tgttcgatga ggtgtacgaa gagtatgtcg
     7621 aggtgccgag cgacgtggtt cccttcgagc agcgtccctg gatgaaagcc gcagagatca
     7681 ccgatgcgac catcaaggcc ctcacgggcg gcggagtacg gcacgcccgc atcaactacg
     7741 ccaatggaga catggtcggt cacaccggca acctggacgc atccgtcatc gccgtggaag
     7801 ccgtcgattt gtccttgggg cgaatcattc ccgtcatcga gaagctaggc ggcgcgctga
     7861 tcgtgacggc cgatcatggg aactgcgacg agatgttcga ggtggacaag aagtcgggag
     7921 cgttcaagcg ggacgctgcc ggacatctca ggagcaagac cagccacacg ctcaacgccg
     7981 taccctgcta cctctacgtg cccgggcacg agactttgcg cctcgacgaa agcatcgcgc
     8041 acccggcgat cacgaacatc tccgcgacga cgttccaact gctcgggtac gaacgccccg
     8101 aaggctacga ttcgtcgctc ctcgaatgac cgcgcgtccg cagggtcagc gataccgaat
     8161 ccgcagcttg atgccgagaa aatcggcgat ctgctgggcg taggagcggg gaatcaaatc
     8221 aagccgaccc tccaccacgg cgcgggcgcg cgcatggggc tctcccttga gtgcagggag
     8281 aacgatctga gtccaggcca ggagcgtctt tccgtcggct cccttgagcg cgagtgtcag
     8341 cacgggctcg agacgcttct ggtaggcgac cgacgctgta caaagtgcgg cgaacgtctt
     8401 cacgagaccg tctttgccga catcgttggc cgttgcgaac gaagtcttga ggacctcgag
     8461 ttgtcgcgcg actcgggcgg gtgcgatgcg tgcgatggcc ggaagagctt ccgccgaggt
     8521 ctgcacgacg cgcttgttcg acgaggacac ccccttcgca aattttgcaa ctagggatac
     8581 gacgagctcg ggcttctcgc tgatcacttc gctcagcacg cgtgcagact gagtcgccgc
     8641 cgttcgcgga ccctccatca tggtttcgcc atgctccgcg atcttcgact gcgggctttc
     8701 gtccgagagg atctttcttg ccgcggcccc agcgcctccg ccggcggact tcttggctgg
     8761 ggttgccgcc actgcgtccg tctcttcttc tttggtcttc ttcttgctcg tcattcgggt
     8821 ccttgaagtg cttcgacgat cagcggcaat gaggcttcgt agcgccacga ggtgcctcga
     8881 tgtccgccct catattcctc atagcgaacg gggagcccat gcttgcgaag cgcctcgtcg
     8941 agcagccgcg ctgcgaagtg tagcccgtgc tcatccctgt tgcctgcatc gacgtaaata
     9001 atcgacatcc tttgcaaagc ggccaaggaa gcctctatcc gcccaagcgg atcctgctcg
     9061 agccatctgg cccagaccgc ctcgtcgatt tgtccggtcg agggatcgac ggggatctgg
     9121 gcaaacggga acgcgccagg gtcgggcgag taggcggctc cagcggcgag cacgaagatc
     9181 gcgtcgaact ccttcgcgtt cttcggtccg gacgctggaa tttcctcggc gaacgcatga
     9241 agcccgccgg cgagatcgat cgcgatggcc gcgctcgtga gcatcggtcg catgctcact
     9301 tcgaaggcgg cgtcgcccgc atgacttccg atcacggaga ccacgtcagg ccggtccatg
     9361 ccgaggcgaa gtgcgccaaa gcccccagac gagcggccga ccacggcccg cgccaggcga
     9421 tcgggaatcg tacggaactc cgcatcgacg aaggcgaaca cctcgtcggc caggtaggtt
     9481 tgataacgcc cggttccttc ggagtcgatg aactgcgagc cgccccagcg gttgaagcag
     9541 tcgggcagga ccagaacggc gggctggcac cgtccttccg ccacgagccg gtcaaacagc
     9601 tcgagtgtgt tcggtttcca agggctccaa gccgcgatcg agtgattggt cgcgccaaaa
     9661 ccggcgagta gcatcaccac cgggaaacgc ttcgattcat cgtactgcgg aggcagatac
     9721 acatagaggt cgcgcctatg gggatcgccg agcgggttgt ccctcagaca ctggctttca
     9781 tgggatgtac gaacgacccg gcccttcatt ggcgggcttc gcatagcaca ttcgccaagt
     9841 cgctgcggga aataccgaga ctttcgctac atgcgatcga gcacgccgat gccaatgagc
     9901 ccgaggccgc gccgaatctg acgtgcggtc agtgccgtga gcccgagacg gctctctctg
     9961 acgtcgccct cggcgtccag cactgggcac tccgcgtaga agccgttgaa cgctctcgcc
    10021 agctcgtaga cgtgctcgca gatcaggtgt ggctggctcg tcgcgccggc ctgatacacc
    10081 acgtcggaga agcgcaggag gtgcttcccg agggtttgct ccgccgctgc gtcgagggcg
    10141 atcgtcgtgc caaccgcgac cgagtccatc tcgattccgc ctttggcgaa gatcgacgcg
    10201 cagcgcgcat atgcgtactg aaggtacgga ccggcatttc cctggaatga aatcatcttg
    10261 tcccaatcga actggtagtc gctcagccgg ttctggcgaa ggtccgcgta cttcaccgcg
    10321 ccgatgccca cgaccttgga aacctcgtcc atctcttcgg ggcgaacgcg cagacgacct
    10381 gcggcgatcc cctcgtcgat gcgttgtcgc gcgcgttcct tcgactcctc gagcaaagat
    10441 gccagcgtga tcaccgcacc gctcgcatct ctcgttctca gcggttttcc atcatcgccg
    10501 agaaccgtcc cgaatccaac gtgctcgagc tccatctcga caccgagcag cttcatgatc
    10561 gtgaagagct gttggaagtg aagcccttgg cgattgtcga ccacgtagag cgcgcggtct
    10621 gcttgaaaat ggtccctccg atactcgacg gtagcgatat cggtcgtcga gtagagaaag
    10681 gccccgtctt tctttcgaac gatgaatggg gtcttctgct tgcgcagcgc tttcggcgcc
    10741 ccgtcgatct cgtcccagaa gatgcaaacg gcgccgtcgt cttcgcgtgc gagcccgcgc
    10801 tccagcaact cgtccacgac tttgggcaaa gcatcgtggt acgcgctttc accgagccag
    10861 acatcgaacg agacatcgag ctcggcgtac acggtctcga gcgccccccg cgtcacctcg
    10921 acgaaatgct cccagagggc gcgattctcc ggatcgccgt tctgaagctt cgcgagctcg
    10981 gcacgtgcgc ggtccgcgaa aacttcctca ttaccggccc gttcggaggc gagcttatag
    11041 acgcgctcga gctctttgat cggatccgat agcagcgcgc tctcctcgcc ccattcccgc
    11101 attcccacga tcaggagccc gaattgagtg ccccagtcgc ccagatgatt gtcacggacg
    11161 acgtcgtgac ccatgaacga caagatccga gcgatcgcgt cgccaatgat cgtcgaccgc
    11221 aggtgcccta cgtgcatctg cttggcgatg ttcgggctcg agaagtcgac gatgatcttt
    11281 tccgccggtt cgacgacggg caccccgtct cgtgatgcat cggtaagcgc ttcgccgagc
    11341 ttcgatgcga cccaagcggg agcgagatga atgttcacga atcccggtcc ggccacttcg
    11401 gccttctcga tggcgtcgat cctgctgagg gcctcggcga tcggctgggc cagctcgcgc
    11461 gggggcttcc ccagctcttt gccgagcggc atcgcgccat tgatctggaa gtcaccgaac
    11521 ttcgggtcct gcgtgggccg aacgatcgcc ggccggggtc catcgagttg gagggcgtct
    11581 tgaatggctt gggagccaag tgactcgagg tagcgctcga ggagcatggg cggaggctta
    11641 ccacgtgcta cgctccggcg ccgtgaatgt cattcgggcg atgatgggtg gtttgaggcc
    11701 cgtgattcga gctcgtcgcg cgatttttgg gccgcggcgg ccgacctgga cgctcgagct
    11761 agaaacggtc gccgaggtgc tgcgacgctc ggccaacctc tcgacggtgc tgccgctcca
    11821 gattcagcgc cgcgtcatcg accctccacg accggtcacc aagccgatgc gcgaagtcga
    11881 catgaagccc gtcgacgccg gcggggtgtc tgccgaatgg ttttctcagc cggccagcga
    11941 tcggagtcgc gtgatcctct atctgcacgg tggcggctac tccacgggct cgatcaactc
    12001 gcatagggac ttcctggccc ggctctgcat cgcgagcggg atgcgcgttt tggcggccga
    12061 ctatcggcta gcgc
//
LOCUS       DASRZF010000332         6887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1633667, whole genome shotgun sequence.
ACCESSION   DASRZF010000332 DASRZF010000000
VERSION     DASRZF010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 6887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6887
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1633667"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="VFG22_14655"
     CDS             complement(<1..713)
                     /locus_tag="VFG22_14655"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET6417535.1"
                     /translation="MIERLVGWSVQNRRLVALLVLLAALFGAVAGSQLRFDALPDVTG
                     KQVIVLTRAEGLTPEEVERLVTRPVEMALGGLPLSKGQRSISRYGLSSVTVLFEDQAD
                     ILQARQLVQERLLNVAGSMPPGVDSPALGPLTGGLGEIYQFTVSSPDKSAAELLELVR
                     FRVMPLLSAVPGVVEVNSWGGERRTMDVVGDAARMARYRITLSDLTEATRKGTGYAAG
                     ATLDAGESGVLLRGVAWPQ"
     gene            complement(710..1768)
                     /locus_tag="VFG22_14660"
     CDS             complement(710..1768)
                     /locus_tag="VFG22_14660"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET6417536.1"
                     /translation="MRIARATVILLALAACTESAAPAGEQEAAPAASAFSRFVAISRP
                     TDASILEAPAVVRAESAASGEITAPTPLRIARVHVQLGDVVSTGDAIVDVYAPEVLDA
                     AAAYLATASQSDTHERRADQLEALLSEGLVDRSEVFQLRARAAELRAARLRAIAILRS
                     SGVDPKDAGKLIERGVVTLRAPVDGVVTELSARVGRSFQPGVTSIARLMGKALARIEV
                     RTAEHWPEASRIVFSAGDGTEIELEPTPVASVVVPEDGTIRSWFAPKDSVALPDGLVG
                     TAKVLAAEDVWQVPSRSIRQQDGESVLMRRRGDTTERIDVEVLTASGTSALVRGPFTL
                     GDLVASSFPPGETRESTP"
     gene            complement(1765..3051)
                     /locus_tag="VFG22_14665"
     CDS             complement(1765..3051)
                     /locus_tag="VFG22_14665"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET6417537.1"
                     /translation="MALEEEIPAPAGRWKRLAIMAIVPVIFSMTDRARAEEISFDQAI
                     ALGEHTPEVSGPRDTLEARESGDRQIGGTAGGTHLIFMPGALLIEGKNEGFEMQATIT
                     QGWSLRDLGGARRDAARSERGVLQASVRAAALRSRLEAARRWIDLATLSEIAEVMESR
                     VRGLEELVESRERALSAGAGTAAPLAEARVMLAELRQSRLDLEGDEFAAATQLSLSVG
                     RAPQAEPLRTAGPMPEPSLPEEPEIRDRMTDVEGAPDLVVERLQETAARARAVEASAQ
                     YAPVLTLGAQGEHSALGPWVIFGVTGLSFQGFGQKHRWSSFAEGEAAGASAATTSARL
                     RVHAELEEAVHELRHSAEVVNVIEGQTLPALRALVASLERAVALGEATRFALIAARDR
                     ELAALGSAYRAHGAVTWAKVHLWLLLAELAVGDGMP"
     gene            complement(3149..4615)
                     /locus_tag="VFG22_14670"
     CDS             complement(3149..4615)
                     /locus_tag="VFG22_14670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417538.1"
                     /translation="MTHGFKGLMLLGLIAGLGLDISSARADVPEAPAPSPVTDADADA
                     DADADTGTAPGTGTAPGTGTAAGTGTGADAVADADTAPDSNPVLDTGTVGGEPTIILA
                     PGEIAPEQPRAGMFEMEVDPMRGLQPARIRREQRTTIGGYGELHLNMEKPEEGSFESI
                     LDLHRFVLFFAHRFNDRFQFYSEVELEHAFVATSGGVAVPGSVQIEQLLIDWRVLRGS
                     SEALYLRAGVILIPMGIVNQWHEPPIFNGVERPQVDRVIIPTTWREGGIGIWGQPTEA
                     FRYELYVVGGLDAAGFSGASGIRGGRLKVTQAVLNGPAFTGRAEWEPTLGMIVGISPY
                     FGWAGPRDLSDVNLRVSGISADWRVLRKGFESRAVIAYFHISDIDALREIPPGETEPT
                     STVGSDLFGVYGELGYNVLYYVDTEMALVPFVRVDYFDTTFRESDSAYDLPAFLVPTI
                     GLTYRPIPQVVFKFDYQYELPSTGLAINRWNFGVGYMF"
     gene            4790..>6887
                     /locus_tag="VFG22_14675"
     CDS             4790..>6887
                     /locus_tag="VFG22_14675"
                     /inference="COORDINATES: protein motif:HMM:NF021355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HET6417539.1"
                     /translation="MRRFYGIPFLIVLGLIGGPIACSNSSEVSDYEAALKQAASCDEV
                     LAAIQEDAIAKVDLELKSFIDQDYYGWGGPVAFGRAEDGAGGASGDPAVPSAGGDSGA
                     EAPSGFSDTNRQVGDVDEADIVKVGDEGRKLYVIRGNGFYEFDSWPAAQTSKVADLEI
                     EGGVMEMFVEGDRAVVFSNTYGVESLDDGALCRDYYGAPGLADYDVAYYCGRSFVKLT
                     VIDLAGDVPEAVREIYVDGWYTSSRRHGNIVRAIVQSSMQRPASVPQLWDIVWGGDVQ
                     PESRDAEILLARAWANKAKEAIRDTTLDQWVPVWGERVDGEIQKQPVQCADFYSPEPG
                     LTNYGLTQIVGLDMASDSATAITSILGQATQVYSNAETLILAQPDWTWVERGTSSDRT
                     AVHRFAVSFEAQRTPYQGSGFVQGIVADQFSIDERAGVIRVATTRTSWPNRDTDPVAP
                     DVWIPPTTDNLVSTLRLNGDALELAGSTPPLAEGERIFSARFIGDLGYIVTFRQVDPL
                     FAIDLSDAENPKVLGELKIPGFSDYMHPLGDDHLLTIGRDIDPDTQVDNGTALQIFDV
                     SDPVNPRLAHKETVGQGYSEANYNHKAFNFYADRGLLAFPFVSYEGDFSSTLELWDVS
                     AADGFTRRGAVDHSDLVLDDCGGVPYPVEDFYYNCGYQPQITRGVFISDDTTDYIYSI
                     SYGGIRVHDMTNLTTPL"
BASE COUNT         1271 a         2252 c         2120 g         1244 t
ORIGIN      
        1 ctttgaggcc aggcgacgcc gcggagcagc acgccggatt cgcccgcgtc cagcgtcgcg
       61 cccgccgcgt agcccgtgcc cttgcgcgtc gcctcggtca ggtccgagag cgtgattcgg
      121 tacctcgcca tgcgcgctgc gtctccgacc acgtccatcg tgcggcgttc gccaccccag
      181 gaattgactt ccaccacacc cgggacggcg ctcaggagcg gcatgactcg gaaccgcacc
      241 aactccagaa gctcggccgc gctcttgtcg ggtgagctga ccgtgaactg ataaatttcg
      301 ccgaggccgc ccgtcagtgg accgagtgca ggagaatcga cgcctggcgg catgctcccg
      361 gcgacgttga gaagacgttc ttgcaccagt tggcgagctt gcagaatatc cgcctggtcc
      421 tcgaagagaa cggtcacaga cgataagccg taccgggaga tgcttcgttg ccctttggaa
      481 aggggaagac ctccaagtgc catctcgacg ggacgcgtga ccagacgctc gacctcttcg
      541 ggtgtgagac cctcggcccg ggtcagcacg atgacctgtt tccccgtgac gtcgggaagc
      601 gcgtcgaatc tcagctgtga gcctgcgacg gcaccgaaaa gcgccgcgag cagaacgagg
      661 agagcaacca ggcgccgatt ttggacggac cagccgacca gcctctcgat cacggcgtgc
      721 tctccctggt ttccccgggc ggaaaggagc tcgcgacgag atcaccgagg gtaaatggtc
      781 ctcgcacgag cgccgaggtg cccgatgccg tgagcacttc gacgtcaatt cgctccgtcg
      841 tatcgccgcg cctccgcatc agcacgctct ctccgtcttg ctggcgtatc gatctgctcg
      901 gcacttgcca aacgtcttcc gcagccaaga cctttgccgt cccaaccaag ccgtcgggaa
      961 gcgccacaga atctttcggg gcgaaccagc ttcgaattgt gccgtcctct ggcaccacga
     1021 ccgaagccac cggggtcggc tcgagctcga tctccgttcc gtctccggcg ctgaagacaa
     1081 ttctgctcgc ctcgggccag tgctccgccg tacggacttc gatccgcgcc agggcctttc
     1141 ccatcagccg cgcgattgac gtcacgccgg gctgaaagct gcggccgact cgcgccgaga
     1201 gctcggtgac cacaccatcc acaggcgccc gcagtgtcac gacacctcgt tcgatcaact
     1261 tgcccgcatc cttgggatcg accccagagg aacgaaggat ggcgatggcc cgaagtcgag
     1321 ccgcacgaag ctccgcggcc cttgcccgaa gctgaaagac ctcggaacga tcgacgagcc
     1381 cttcggagag caatgcttcc aactggtcgg cacgccgttc atgggtgtca ctctgggatg
     1441 cagtcgcaag gtaggcagcg gccgcgtcca gcacctcggg cgcgtagacg tcgacgatcg
     1501 cgtctccggt ggatacgacg tcccctagct gcacatgaac gcgcgcgatg cgtagggggg
     1561 tcggcgcggt gatctcgccc gatgcagccg actctgcgcg cacgaccgca ggagcctcga
     1621 ggatcgatgc gtcggtcggt ctcgagatcg cgacgaacct cgagaaagcg ctcgctgccg
     1681 gagctgcttc ctgctctccg gcgggcgctg ccgactcggt gcacgccgct agagcgagca
     1741 ggatgacggt cgctcttgca atcctcatgg catgccgtcc ccgaccgcga gctcggccag
     1801 aaggagccac aagtgcacct tggcccaggt caccgcacca tgcgcccggt atgccgatcc
     1861 aagggcagcg agctcccgat cgcgggcagc gatcagcgcg aatcgcgtcg cctcgccgag
     1921 cgcgacagcg cgctcgaggc ttgccaccag tgcacgcaaa gcggggaggg tttgcccctc
     1981 gataacattg acgacttcgg cgctgtgccg gagctcgtgc actgcttctt cgagctcggc
     2041 atggactcgc agtctagcgc tcgtcgtcgc cgcgctcgcg ccagcggcct ctccctctgc
     2101 gaaggagctc caacgatgct tctgcccgaa tccctgaaaa ctcaaaccgg taacgccgaa
     2161 gatcacccaa ggcccaagcg cactgtgctc cccctgcgcg ccgagggtca gcactggcgc
     2221 atactgtgcg gacgcttcga ccgctctcgc gcgggctgcg gtttcttgga gccgctcgac
     2281 gacgaggtcg ggtgctccct cgacgtcggt catccgatcg cgaatctcag gttcctccgg
     2341 cagagatggc tccggcatcg gacccgccgt gcggagtggc tccgcttgag gtgcgcgccc
     2401 tacggagagc gatagctggg tggcggcggc gaactcgtct ccctcgagat ccaggcggct
     2461 ctgccggagc tccgcgagca tgacccgggc ttcggccagc ggcgctgcgg ttccagcgcc
     2521 agccgacaga gcgcgctccc ggctctcgac gagctcttcg aggcctcgca cccgtgattc
     2581 catgacctcc gcgatctcgg aaagcgtggc gaggtcgatc cagcgccgcg cagcctccaa
     2641 acgcgaccga agggcggcgg cccgaaccga cgcttgcaag acaccccgct cgcttctcgc
     2701 tgcatctctg cgggcgcccc cgaggtcacg gaggctccag ccctgcgtga tcgtggcttg
     2761 catctcgaag ccctcgttct tgccctcgat gagcagcgct cctggcatga agatcagatg
     2821 ggtcccacct gctgtgccgc ctatctgtcg atcacccgac tcgcgcgcct ccagcgtgtc
     2881 tcgcggaccg gaaacctctg gcgtgtgctc tccaagagca atagcctgat cgaagctgat
     2941 ctcctcggcg cgcgcacggt ccgtcatcga aaagatgacc gggacgatcg ccatgatggc
     3001 gagtctcttc catcggccgg ccggcgccgg aatctcttct tcgagggcca tcgaagtcgg
     3061 acgctttgca ccagccagct ccaaggtcca tccatcggat gttacatacc gcgcatcccc
     3121 atgctttcac tgcgcaatct tggactgctt agaacatgta gccgactccg aagttccagc
     3181 ggttgatggc caggcccgtg gatggaagct cgtactggta gtcgaacttg aagacgactt
     3241 gcgggatcgg ccgataggtc aggccgatgg taggcaccaa gaaggccggc aggtcgtatg
     3301 ccgagtccga ctcgcggaac gtggtatcga agtagtcgac ccggacgaat ggaacgagcg
     3361 ccatttcagt gtcgacgtaa tagagcacgt tgtaaccgag ctctccgtag acgccgaaga
     3421 ggtccgagcc gacggtgctg gtcggttcgg tctcgccggg cgggatctct ctcagggcgt
     3481 cgatatcgct gatgtggaag tacgcaatca ctgcgcggga ctcgaatcct ttgcgcagga
     3541 cccgccaatc cgccgagatc ccgctcaccc tcaagttcac gtctgaaagg tctcgaggcc
     3601 cggcccatcc gaagtagggc gaaataccca cgatcatgcc gagagtgggc tcccattccg
     3661 cgcgcccggt gaacgccgga ccatttagaa ccgcctgggt gactttcaac cgtcccccgc
     3721 gaatgccgct tgcgcccgag aatcccgcgg cgtcgaggcc cccgaccacg tagagctcat
     3781 agcgaaacgc ttccgtgggc tgaccccaaa tcccgattcc tccttcgcgc caggtggtcg
     3841 gaatgatcac ccgatcaacc tgggggcgtt cgacgccgtt gaagatcggc ggctcgtgcc
     3901 actggttgac gatgcccatc gggatcaaga tcacgccggc tcgtagatag agtgcttcgc
     3961 tcgatcccct gagcacgcgc cagtcgatga gtagctgctc gatctgtacg gagcctggaa
     4021 cggcaacacc gccgcttgtc gcgacgaacg catgctccag ctcgacctcc gaatagaact
     4081 gaaatcggtc gttgaaccga tgcgcgaaga aaagaacgaa acggtgtaga tcgagtatcg
     4141 attcgaagct cccttcttcg ggcttttcca tgttcaggtg aagctcaccg tatccgccga
     4201 tcgtcgttcg ctgctctcga cggatgcgtg ccggttgcag gccacgcatg ggatcgactt
     4261 ccatttcaaa catgcccgcc cttggttgct ccggcgcgat ctcgccggga gccaagatga
     4321 tcgtcggctc tccgccgacc gtcccagtgt cgaggacagg gttgctgtcg ggcgcggtgt
     4381 cggcgtcagc gaccgcgtca gcgccagtgc cagtgccagc ggcagtgcca gtgccagggg
     4441 cagtgccagt gccaggggca gtgccagtgt cagcgtcggc gtcggcgtca gcgtcagcgt
     4501 cagtgaccgg ggagggggcg ggcgcctccg gcacgtctgc ccgcgccgac gagatgtcta
     4561 ggcccagccc cgcgatgagg ccaagcagca tcaaaccctt gaatccatga gtcattggcg
     4621 accgctcctt gaagcaaatg aaaatgattt gcaacttcac tagcatgtcg caaaagacct
     4681 gcaaggatga tcgagatggc acagcctggc tcagtggtta tcaagagatc acagggtttt
     4741 tcgccaggca cgctgtttgc ttgtcttaga cgcagctagg aggacgagga tgcgtaggtt
     4801 ttacggaata cctttcttga ttgttttggg gctgatcggc ggcccgatcg cgtgcagcaa
     4861 cagctcggag gtgagcgact atgaggctgc cctgaagcag gcggccagtt gcgatgaggt
     4921 gctcgccgcg attcaagagg acgcgatcgc caaggtcgac ttggagctca agtccttcat
     4981 cgatcaggac tactacggct ggggcggccc cgttgcgttc gggcgcgctg aggatggcgc
     5041 aggtggggcc tccggcgacc ctgcggtccc gagcgcggga ggtgacagcg gggccgaagc
     5101 gccatcgggc ttctccgaca ccaaccgcca ggtcggcgac gtcgacgagg ccgacatcgt
     5161 caaggtcggt gacgaagggc gcaagctcta tgtgattcgc ggcaacggct tttacgagtt
     5221 cgattcctgg cccgccgcgc aaacctccaa agtcgcggat ctcgaaatcg aaggtggcgt
     5281 gatggagatg ttcgtcgaag gcgatcgtgc cgtggtgttc tcgaatacct acggcgtgga
     5341 gtcgctcgac gatggtgccc tctgtcgtga ctactacgga gctccggggc tcgccgatta
     5401 tgatgtcgcg tactactgcg gtcgctcctt cgtgaagctc accgtgatcg atctcgctgg
     5461 cgacgtcccc gaagcggtcc gcgagatcta tgtggacggc tggtacacct cctcgcgacg
     5521 ccacggcaac atcgtccgcg ccatcgtgca gagcagcatg cagcgcccgg cgtcggtgcc
     5581 acagttgtgg gacatcgtct ggggcggcga cgtacagccc gaatcgcggg acgcagagat
     5641 ccttctcgcc cgcgcatggg cgaacaaagc caaggaagcg attcgcgata cgacgctcga
     5701 tcaatgggtg cccgtttggg gtgagcgcgt cgacggcgaa attcagaagc agcccgtcca
     5761 atgcgccgat ttctactctc cggagccagg tctcacgaac tacggactca ctcaaatcgt
     5821 cggcctcgac atggcgagcg acagcgcaac cgcgatcacc tcgatcctcg gccaggcgac
     5881 tcaggtctat tccaatgctg aaaccctcat cctcgctcaa ccggactgga cttgggtcga
     5941 gcgcgggacc agcagcgatc gcacggccgt tcaccgcttc gcggtctcgt tcgaggccca
     6001 gcggacgcca tatcagggct ctggattcgt ccaagggatc gttgcggatc agttctccat
     6061 cgacgagcgt gcaggagtga ttcgagttgc gacgacccgg accagctggc ccaatcgcga
     6121 caccgatccg gtagcgcctg acgtttggat tcctccgacc accgacaacc tggtcagcac
     6181 tttgaggctc aacggcgacg cgctggagct cgcgggctcc acaccgccgt tggctgaggg
     6241 ggagcggatc ttctccgcgc gcttcattgg agacctcggg tacatcgtga cgtttcgtca
     6301 ggtcgaccct ctctttgcca tcgacctcag cgacgccgag aacccgaagg tcctcggcga
     6361 gctcaagatt cccggcttca gcgactacat gcatccgctg ggcgacgacc atctactgac
     6421 catcggtcgc gacatcgacc cagacaccca ggtcgacaac ggcacggctc ttcagatctt
     6481 cgacgtgtcc gacccagtga atccgcggct cgcgcacaag gagaccgtcg ggcaggggta
     6541 ctcggaagcg aactacaacc acaaggcgtt caacttctac gccgacagag gactgctagc
     6601 gtttccgttc gtgtcctatg agggcgactt ctccagtacg ctcgagctct gggacgtcag
     6661 tgctgcggat gggttcaccc gccgcggcgc cgtcgaccac tccgatctag ttctcgacga
     6721 ctgcggagga gtcccgtacc ccgtggagga cttctactac aattgcggct accagcctca
     6781 gatcacgcgg ggcgtcttca tcagcgacga taccacggac tacatctatt cgatcagcta
     6841 cggcggcatt cgggtgcacg acatgaccaa tctcacgacg ccgctgg
//
LOCUS       DASRZF010000333         5526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4387483, whole genome shotgun sequence.
ACCESSION   DASRZF010000333 DASRZF010000000
VERSION     DASRZF010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 5526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5526
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4387483"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="VFG22_14680"
     CDS             complement(<1..662)
                     /locus_tag="VFG22_14680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HET6417540.1"
                     /translation="MSERIAVVGLGYVGLPVALAFARKFEDTVGFDISEARVNALRAN
                     EDWTGEVSGAVLEASPLKLSSDPADLRGCTFFVVAVPTPIDSDRRPDLGPLFSASRIV
                     GSALAPGAVVVYESTVYPGVTEEQCGPLLAAESGLRQTHDFKLGYSPERINPGDKLHT
                     FEKIVKVVAGEDAESLERVARAYESVVDAGVHRASSIKVAEAAKVIENTQRDVNIALM
                     NEL"
     gene            complement(659..1603)
                     /locus_tag="VFG22_14685"
     CDS             complement(659..1603)
                     /locus_tag="VFG22_14685"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET6417541.1"
                     /translation="MKILVTGGAGFIGSHVAEAAVEAGHEVLIVDDLSTGRTENLPAR
                     VDFHPLDIRNREALRELALHFRPDAISHHAAQASVTMSVRDPILDADVNVMGSLNVAN
                     VAIESGSRLIFASTGGALYGEVPEGKSAGEDWPARPLSPYACSKASFELYFRAFGQAS
                     DLRYTVLRYANVYGPRQDPHGEAGVVSIFLQRLLRGEPIQVNARIKKGDGGCVRDYVY
                     VRDAVRANLAAFEGALDGCTINVGTGQPTSTRALAETLVDFVDRPAIVNDGPHRSGDL
                     ERSVLDPTVMVSILGEPTPLARGLTATTEWFRDRVAST"
     gene            complement(1600..3534)
                     /locus_tag="VFG22_14690"
     CDS             complement(1600..3534)
                     /locus_tag="VFG22_14690"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HET6417542.1"
                     /translation="MSRLKTRTSQFLIDIAVLRFAFVLAMLVRFDWDVPWVMFKQLMI
                     VLPYVVLLQYGFLSAFGTTRFSWRYISLRDTVRIFGAIASAAALLVALRLLSPVLVDR
                     FPLARYGIAPLGVLLGDFVLAFLGLTGVRALRRMLGERVASKQHQGDGEPQEPTLLVG
                     AGQGGFLVAQEIARRPDLGILPVAFVDDDPVKQGAVIHGIKVKGTTQDLLRLAERHSA
                     KQALITISNAPGAAIRRITESCKQAGLTVKIVPGTYEIVGGRVNLSRIREVAIEDLLR
                     REPVKLNTEAVSGVVRGQPVMVTGAGGSIGSELCRQVAAYRPSRLVLVERSENNLFEI
                     HSELQNRFSEVEIIPCVADITDRPRIAQVFHDHRPMVVFHAAAHKHVPMMEWNPAEAA
                     KNNVLGTQIVADLADSLAVERFVMISTDKAVNPSSVMGATKRAAELYVQALSLNSATH
                     FATVRFGNVLGSAGSVIPIFRRQIEDGGPITVTHPGMTRYFMTIPEAAQLVVQAAALS
                     RGGETFILDMGQPVRILDLAHDLIRLSGLEPDKDIEVRFTGIRPGEKLFEELSNMAEG
                     IQRTSHPKIFTGTYDSPHLPRLRAQFDTLGALCRTADPSAIRQALEQLVPEYAKGDAD
                     SEPPARSRTMMPDSKSMGSL"
     gene            complement(3531..4511)
                     /locus_tag="VFG22_14695"
     CDS             complement(3531..4511)
                     /locus_tag="VFG22_14695"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET6417543.1"
                     /translation="MKKILLTGAAGFIGSNTASALLRRGDTVVGVDNLNDYYDPARKE
                     ANLEEVRASAPDVRKFIFHREDIRDEGAMDSLFAEHGFDAVIHLAALAGVRASIDDPK
                     LYLDVNLGGTLSLLEAANRHGQPLFVFASTSSVYGRTKVIPFVETDRADRPLAPYPAS
                     KRAAELLGYSYFHLFGQNFTALRFFTVYGRRGRPDMMAYKVLDNIFRGAEVPLYNNGQ
                     MHRDWTYVDDIVEGVIAAADRPLGYEIINLGRGQPTLLADFVGLIEKMAGKRSNLASK
                     PMPAADIEYTYANIYRAKELLGYAPKVTVERGVEEFWAWYRESVLKGEGR"
     gene            4586..>5526
                     /locus_tag="VFG22_14700"
     CDS             4586..>5526
                     /locus_tag="VFG22_14700"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HET6417544.1"
                     /translation="MLPVQAMLLAAGLGTRLRPLTDLRPKPIVPLANRPLAGFAMEHL
                     ASCGVQHIVANTHPHAELVESALKAACPSDVSLRFSREARLLGTGGGLRKARPLFDDP
                     GSPVIVMNGDTLFAPDLAKALVKHEARGAVATMILRPTNEPERFGVIGIDAEGWVRSL
                     LGAPRPLPGHRALMFTGIHILAPSAWAQMPESGCVIRSAYRYWIDSGAPVLGIVDESP
                     WADLGTVSEYHRLNLELASGSFAWPGVRPRAGCILPDDLDPSEQVRRSVIGHDVQLAE
                     GVTLDCCVVWPHSNVRRSASHAVITPEHRIELSIPDDL"
BASE COUNT         1033 a         1770 c         1701 g         1022 t
ORIGIN      
        1 agctcgttca tcaaggcgat gttcacatcg cgctgcgtgt tctcgatgac cttggccgcc
       61 tccgcgactt tgatcgacga ggctcgatgc acgccggcat cgacgacgct ctcataggcc
      121 cgggccacgc gctccagcga ctctgcgtct tcgcccgcaa ccaccttgac gatcttttcg
      181 aacgtgtgga gtttatcgcc agggttgatc cgttccggag agtatccgag cttgaagtcg
      241 tgtgtctgtc gaagccctga ttccgccgcg agaagcgggc cacactgctc ctcggtgact
      301 cccgggtaaa cggtcgactc gtagaccacc acggcacctg gagcgagcgc gcttccgacg
      361 attcgactcg ccgagaagag cggcccgagg tcagggcgtc gatccgagtc gatcggcgtg
      421 ggcaccgcga cgacgaagaa ggtacagccc cgaaggtccg ccgggtcgct gctgagcttc
      481 aaggggctcg cttcgagcac cgctccgctc acctccccag tccagtcctc gttcgccctt
      541 agcgcgttga ctcgagcctc tgagatgtcg aagcccaccg tgtcctcgaa cttccgagca
      601 aacgccaagg ccactggaag acctacgtac ccgagcccca caactgcgat tcgttcgctc
      661 atgtcgatgc cacccgatct cgaaaccact cggtcgtggc cgtcaagcct cgtgctagcg
      721 gcgttggctc cccgagaatc gaaaccatca ccgtggggtc cagcacagaa cgctcgaggt
      781 cgccagagcg atgcggcccg tcgttcacga tcgccggccg gtcgacgaag tcgaccagcg
      841 tttcggcgag cgcccgagtg ctcgttggtt ggcccgtgcc gacgttgatc gtgcagccgt
      901 cgagagcgcc ctcgaatgcg gcgagattcg cgcgaacagc gtcacgaacg tagacgtaat
      961 cacggacgca gcctccgtct ccctttttga ttcgcgcgtt gacctggatc ggctcgccgc
     1021 gaagaagcct ctggaggaat atcgaaacga cgccagcctc accgtgcggg tcctgcctcg
     1081 gcccgtagac gttggcgtag cggagaaccg tgtaccgcaa gtcagaggcc tgaccaaagg
     1141 cgcggaaata gagctcgaag ctcgccttac tacaggcata cggactcagg ggacgcgcag
     1201 gccagtcttc gccggcggac ttgccctcgg ggacttctcc atagagcgcg ccacctgtac
     1261 tcgcgaaaat caaccggctc ccagactcga tcgccacatt ggcaacgttg agagaaccca
     1321 tcacgttgac gtcggcatcc aggatcggat cgcgcacgct catcgtgacg ctggcttgag
     1381 cggcgtgatg gctgatcgcg tcgggacgaa aatgcagcgc gagctcccga agcgcttcgc
     1441 ggtttcggat gtcgaggggg tgaaaatcga cacgcgccgg aagattttca gtgcgcccgg
     1501 tgctgaggtc gtcgacgatc aggacttcgt ggccggcctc gacggccgcc tcggcgacat
     1561 ggcttccgat gaagcccgca cctcccgtca cgagaatctt cacaggcttc ccatcgactt
     1621 cgaatcgggc atcatcgtgc gcgatcgcgc agggggttcg ctgtccgcgt cccccttggc
     1681 gtactcgggg accagctgct cgagtgcctg gcgaatggcg ctcggatcgg cggtcctaca
     1741 aagcgcgccg agcgtgtcga attgggcgcg caacctagga aggtgcgggg agtcgtaggt
     1801 tccggtgaaa atcttcggat ggctggtcct ctgaatgccc tcggccatgt tcgaaagctc
     1861 ctcgaagagt ttttcacccg gacgaatgcc tgtgaaccga acttcgatgt ccttgtccgg
     1921 ctccaggccg ctcagccgaa tgaggtcgtg ggcgaggtcg aggatgcgga cgggttgccc
     1981 catgtccaag atgaacgtct cgccaccgcg actcaacgct gccgcctgga ccaccaactg
     2041 cgcggcctca gggatcgtca tgaagtaccg tgtcatgccc gggtgggtga ctgtgatcgg
     2101 gccgccatct tctatctgtc tacggaaaat ggggattacg cttccagccg agccgagcac
     2161 gttcccaaag cgcaccgtcg cgaagtgagt ggcgctgttg agcgaaagcg cttggacata
     2221 gagctccgcc gcacgtttcg tcgcgcccat caccgagctt gggttcaccg ccttgtcggt
     2281 cgagatcatg acgaagcgct cgaccgccag gctgtcggcg agatccgcga caatttgagt
     2341 gccgagcacg ttgttcttcg ccgcctcggc cgggttccac tccatcatgg gcacgtgctt
     2401 gtgggcggcg gcatggaaga ccaccatcgg acggtggtca tggaacacct gcgcaatacg
     2461 gggccgatcg gtgatgtccg ccacgcaagg aatgatctcg acttcggaaa atcggttctg
     2521 gagctcggag tgaatctcga acagattgtt ctccgaacgc tctaccagga cgagacggga
     2581 agggcgatac gcagccacct ggcggcagag ctccgaaccg atgcttccgc ccgcgcctgt
     2641 caccatgacc ggctgtcccc taacgactcc ggaaaccgcc tcggtgttca gcttcacggg
     2701 ctcgcgacgc agcaggtcct cgatcgcgac ttcgcgaatt cgcgacaggt ttactcggcc
     2761 gccgacaatc tcgtatgtgc ccgggacgat ctttacggtg agcccggcct gcttgcagct
     2821 ttcggtgatt cgcctgatcg ccgcgcccgg tgcgtttgaa atcgtgatga gcgcctgttt
     2881 ggcagagtgg cgctcggcca ggcggagcaa gtcctgggtc gttcccttga ccttgatccc
     2941 gtgaatgaca gcgccctgct tgaccgggtc gtcgtcgacg aacgcgaccg gcaagatgcc
     3001 gaggtcggga cgacgcgcga tctcctgcgc aaccagaaaa ccgccttggc ccgcccccac
     3061 cagcagagtc ggctcctgcg gctctccgtc gccctgatgc tgcttcgacg ccacgcgctc
     3121 gccgagcatc cgtcggagcg cacggactcc ggtcaagccg aggaatgcca agacgaaatc
     3181 ccccagcaag acgccaaggg gtgcgatgcc gtagcgtgcg agcggaaacc ggtcgaccag
     3241 gaccgggctg agcaatcgaa gcgccacgag aagggccgcc gccgaggcga tggctccgaa
     3301 aatccgaacc gtgtctcgca gactgatgta gcgccacgaa aagcgcgtcg ttccgaatgc
     3361 ggagagaaaa ccgtactgca ggagcacgac gtacgggagc acgatcatca gctgcttgaa
     3421 catcacccag ggcacgtccc aatcgaaccg gacgagcatg gcgagcacga acgcaaacct
     3481 cagcaccgcg atgtcgatca agaactggga agtacgggtt ttgagcctgc tcatcgccct
     3541 tcgcccttca aaacggactc gcgataccag gcccaaaact cttccactcc ccgctcgacc
     3601 gtcacctttg gagcgtaccc gagcagttcc ttggcgcggt agatgttggc ataggtgtat
     3661 tcgatgtccg ccgccggcat cggcttgctg gccaagttcg aacgcttgcc cgccattttc
     3721 tcgatgaggc ccacgaaatc tgccaggagc gtcggctgcc cccggcccag gttgatgatc
     3781 tcgtaaccga gcggccggtc cgccgcggcg atcacgccct ccacgatgtc gtccacgtac
     3841 gtccagtccc ggtgcatctg gccgttgttg tacaaaggca cctcggcgcc tcgaaagatg
     3901 ttgtccaaga ccttgtacgc catcatgtct ggcctgccgc gccggccgta gaccgtgaag
     3961 aaccgaagcg cggtgaagtt ctgaccgaat agatgaaagt acgaatagcc caagagctct
     4021 gcggctcgtt tgctggctgg atacggagcg agcgggcgat ccgctcgatc ggtttcgacg
     4081 aatggaatga ccttcgtccg tccgtatacc gaggaggtcg aagcgaagac gaacaagggc
     4141 tggccatgcc ggttcgccgc ctccaggagg ctcagggtgc ctcccagatt gacgtcgagg
     4201 tagagcttgg gatcgtcgat ggaagctcgc acacccgcca gcgcggcaag gtgaatgacc
     4261 gcatcgaagc cgtgttcggc gaagagcgag tccatggcgc cctcgtctcg aatgtcttcc
     4321 cggtggaaaa tgaatttcct gacgtctggc gctgatgcac gaacctcctc gaggttggct
     4381 tccttgcggg cagggtcgta gtagtcgttg aggttgtcga ctccgacgac cgtatcgccg
     4441 cgccgcagca gcgcggatgc ggtgttgctc ccaatgaagc cggcagcccc agtcagcaag
     4501 atcttcttca tcgccccctc gtgcccagtt agtataggcc cttatcgcgc cagattgctt
     4561 ggtccgattg ctgcggggga ataccatgct ccccgtgcag gccatgctct tagcagccgg
     4621 gctcggaacg cgcctccgac cgctcaccga cctcaggcca aagcccatcg ttccgttagc
     4681 aaatcggccg cttgcgggct ttgccatgga acatctggcg tcttgtgggg tgcaacacat
     4741 cgtagccaat acccacccac atgccgagct cgtcgaatcc gccttgaaag ccgcgtgccc
     4801 gagtgacgtg agcctgcggt tttcccgtga agccaggctt ctcggtaccg gcggtgggct
     4861 gagaaaggcg cggcccctgt tcgacgaccc cgggagcccg gtgatcgtca tgaacggaga
     4921 taccctgttc gcccccgatc tcgcgaaggc gcttgtgaag cacgaggcga ggggagctgt
     4981 tgcaacaatg atactccgcc ccacgaatga gccggaacgc ttcggggtga tcggaatcga
     5041 cgcggaaggc tgggttcgaa gtctgctggg cgcgcctcgc ccgctgccgg gccatcgagc
     5101 tttgatgttc actgggattc acatcttggc gccgtcggcc tgggcgcaga tgcccgaatc
     5161 gggctgcgtc atccgcagcg cctatcgcta ctggatagac agcggcgcgc cggtgctggg
     5221 catcgtcgac gaatctccct gggcggatct aggcacggtt tcagagtatc accgactgaa
     5281 cctagagctc gcctcaggtt cgtttgcatg gcctggcgtc cgcccgcgcg ccggctgcat
     5341 cctgccggac gatctcgacc ccagcgagca ggtccggcga agcgtgatcg ggcacgacgt
     5401 gcaactcgct gagggcgtca ctctcgactg ctgcgtcgtc tggccgcaca gcaacgtacg
     5461 gcgctccgcg agccacgcgg tgatcacccc ggagcaccga atcgagctga gcatcccgga
     5521 cgatct
//
LOCUS       DASRZF010000334        18595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4727053, whole genome shotgun sequence.
ACCESSION   DASRZF010000334 DASRZF010000000
VERSION     DASRZF010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 18595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18595
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4727053"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(396..608)
                     /locus_tag="VFG22_14705"
     CDS             complement(396..608)
                     /locus_tag="VFG22_14705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417545.1"
                     /translation="MNAPSRRCSPAAPIRMRRPLPGPSIRSRFDPSTGDAELIVPFIK
                     AGEQSIGDFAVGPEDGLLYVIDRFTV"
     gene            798..2201
                     /locus_tag="VFG22_14710"
     CDS             798..2201
                     /locus_tag="VFG22_14710"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET6417546.1"
                     /translation="MEQPDVDPAILRAVGDVGRPERPYEDLASLRHIPGKDGAITGVM
                     NLWGWMRHGRAHLEAQDKKYGPIYRIRVGPTVYIAVNDPTIGAQILRNADGAWSAALG
                     NLVLYGGIPPDGPMDAPVTLDFEPHRDVRRLLQPAFKADALASYVETANRHLATATDR
                     WVTAREVKFKPEARGLFARVAADVFLGVEDQERAEELDKAMSDFWHGITAVVRNEWLS
                     PTWRAARRQYRGLIERFRAQVAARRATDGVDLFTRLCQTNEKVDWLDDEGLVRVLTGL
                     MAAAFDTTSAAVTSMAYVLATDPEWQESLRAEAIDLETETVTYEQIKDLNQLDWVWKE
                     TLRFYPVVPTVWRLALRDVDVGDYTIPAGGLIMVGISMALRDPSSWKYPNRFDPERFS
                     PPRQEDKHNRAAFLPFGAGHHACIGSQLAGLEAKAFWYQFLRKARIRLARPYGARHGY
                     GPLGMVSGDVTLVVEPV"
     gene            2593..2757
                     /locus_tag="VFG22_14715"
     CDS             2593..2757
                     /locus_tag="VFG22_14715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417547.1"
                     /translation="MFLLGSYRTGCRGGIDAALWTIQDGPHVPFFYSPGGEEPAFTDL
                     VTDWLLEHSR"
     gene            complement(2795..4258)
                     /locus_tag="VFG22_14720"
     CDS             complement(2795..4258)
                     /locus_tag="VFG22_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417548.1"
                     /translation="MNRFLLLPSALVALSAAACSSNDSGGTAPADPFAPQADTSEGLT
                     NVSADLMEVLEDGALHGACEAYWDAVADGTDTRRARLLCGKEMFFYEGFDTVGVPTVL
                     LETNMALFPDLIGAGATGVGMIEDPTSADGLPLGFGPGKDFAEGVPSLAFTCASCHFG
                     QLPDGRYVMGAPNHDFEYGELNLAIAVLPTATILQQDLDPAAEAKLAPYFQAIEDDPS
                     IVDGLIASLAPLIGAGASIPEFSAETQAAYAGWKSGTMDFVITPLPLDDEVHTVSKIM
                     SLWGIADEADVAAYEMDGALLAHTGVTASVANFLEGFVILGDGDLDAWDAERLGPLDD
                     YLHSLRAPSNPEPSDAVRVAEGQQLFGTADCIDCHDGARGSSRTVFTFDEIGSDTAMM
                     YWLDRDLDGQPCCDVDLLGRDALTHGLRAQRLTGVWSAKRLLHNGSVDSLEDLFCVNG
                     MRPTITESPYSDRGHMFTCDGLSETEKAALIAYLRSL"
     gene            complement(4255..4425)
                     /locus_tag="VFG22_14725"
     CDS             complement(4255..4425)
                     /locus_tag="VFG22_14725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417549.1"
                     /translation="MTIRLLLNLGQIFCTRGEAETHAAAHTLVLVWAASGGFDSRLAV
                     LRAPRSTLEILG"
     gene            complement(4538..4612)
                     /locus_tag="VFG22_14730"
     tRNA            complement(4538..4612)
                     /locus_tag="VFG22_14730"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4578..4580),aa:Gly,seq:gcc)
     gene            complement(4706..6274)
                     /locus_tag="VFG22_14735"
     CDS             complement(4706..6274)
                     /locus_tag="VFG22_14735"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6417550.1"
                     /translation="MSYSLQIGVRYLRSKKRATVSMITLISIIGVALGVAALLAVLSI
                     TTGFEKAFRDKVLGVNAHVLVLKYGREFRDYRDVLSTVGEMPEVAGVAPFSINEMMLT
                     KGERRSVVLVKGVTPEGVRTVLDLPNQMVAGGLEGLRRPGSTPTRAVEPAEDQDWQWL
                     RDLRDGKTESTKPPSPKAEQAPVQRKTFWEEEKERRAAEREAAERARRPRIGAVAAPV
                     EIETMLGELDEEALALPDDPAWESGLSYGAAQEPIDSLPGIIVGKTLAEDLELELGDR
                     VRLISPMAALGLALSEPSGSTTRSREFRVIGVFQAGFQEYDSGLVYADLFEAQRLFDE
                     GDNVTGVELRVHDLETSYELARRIEQDLGGAFHSLDWSELNRNLFTALEVQKIALTLV
                     IATIIFVAAFNVIATLIMIVIEKKREIAILKAMGATDGTVLGIFMVQGVVVGVIGTLA
                     GVLVGGGLVAYLDKIRFPLDPKVYLIDHLPVVLNPTVFLVTIGIALLICTVSTIAPSW
                     WAARMLPVDGLRYE"
     gene            6370..8571
                     /locus_tag="VFG22_14740"
     CDS             6370..8571
                     /locus_tag="VFG22_14740"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HET6417551.1"
                     /translation="MKRIWPFAMSIGLLVACHQQQNVRREVWPSANQPKPPAAAAEAP
                     TPPPKVLDPIVVVLPEKSPVMQVRKQLDKGNPGKAREVAEAALPAASARDKGRLHWLA
                     AKAALDEGYSSIALAHLDTLSNFNHPLARWAKLRRASLLERTDPVTAAQIAASLTDDW
                     AGSKRARTIEVRSGGSADVSDAHLEIRLDGTLPAKTSEAPAPQQDVRPRMQQSLDKAQ
                     ALYDSKRYKEAERLYGKLVKHLKTGSSAWCDARYKQGRALLQDRERTRGAPVMREVAD
                     KCGRADPETRTWARYHSARAYGRIGDWNESIRQYAKLEAEDPAHSLADDASYRAALAE
                     LENGRPKAAIARLSSIPRRYPEGDMWPRALFKLGWVHLRDGDYENAYERFDELVARGG
                     DEREEGITGRANYWRARTLFMMGRRIDAIAAYEEIVQRWPLRYYAQQALWRIQEIVPA
                     QAEALLVQMRAGDERTRMVFAWRDEFDDPAFDRMLELLRVNEITYASSEMSTLHMLGK
                     NVDPEMAVVGAVLLQHAGAETKLSRVVRTHFEDFEGLLPKGEGKLIWEATYPQAFSPL
                     IEEIASHEGIPASFVRAIAREESSFDPEAVSWAKAYGLVQLIMPTAKRFANEVGEKAT
                     PRTLKKPEVNLRIGARYMAWLWERLGENPALVPSAYNAGEGAVRRWLEEDAARPLDVF
                     IEEIPYDETRRYTRRVLQTYGVYQWLADEKLPALPAKLPSTNAPQRFSALR"
     gene            complement(8576..10351)
                     /locus_tag="VFG22_14745"
     CDS             complement(8576..10351)
                     /locus_tag="VFG22_14745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HET6417552.1"
                     /translation="MMQPLTGLDSSFLYLEDPHQPMHVGSVLVFEGSMTFDEFRETMA
                     SRVHLVPRLRQRLAMVPFGIGKPYWVEDPDFNLDMHLQHVALPSPGSWKELRRLAARI
                     FSVPLDRSRPLWEMTFVEGLDQIPQVPPGSIATINKVHHSAIDGVSGSDMLSVLLDMT
                     KEPRRFIPPPPRAIPPMPNELQVLSHTARKIMGKPTEIKRVAGEINEALRAAAKVKSK
                     GVDAPPVPMTAPHTPINQRVTGQRIWNTALLELDRVKEIRKITGCTLNDVVVAICAGA
                     LRRYLEEKDALPDKPLIAMIPVSTRGKDEHGKMGNKVSAMFLDIATDVADPVERLKVI
                     QEHGKGGKAFEKAGATRAFVDLWDFIPFGLANRVLRLYSRFRVSDMHNPVFNVVITNV
                     PGPQMDMYMAGHKLLATMGMAPLVDGMGLLITVLSYNGVLSISPTSCPKVMPDLDLFT
                     RYLRESANELEAAVLALRKPEDEASRAQSKAVAEMTAAFVEQMRSTLAAAPAERSLGE
                     GTFQLRVTGAADQSWMIDLQERSVTEGNGRPTDATLTIRDAHLAEILRGKLDPQIAFV
                     QGKLRVDGDINKAIEFGALLPKVVA"
     gene            complement(10424..11518)
                     /gene="dnaJ"
                     /locus_tag="VFG22_14750"
     CDS             complement(10424..11518)
                     /gene="dnaJ"
                     /locus_tag="VFG22_14750"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HET6417553.1"
                     /translation="MSKPDYYEVLEVSREVTVTELKKAYRKKALELHPDRNPDNPEAE
                     EKFKVCAEAYSVLSDPQKRDVYDRFGHAGLSGGGGPGFADIGDVFSQFQDIFGDLFGG
                     SGGFGFGGFGRRRRDPNGPQRGADIRTDLSLSLREAAFGSSREIDLSHPSPCEACHGT
                     GAKDGELEGCATCGGRGQVARAQGAFVMTTGCPTCQGRGVRAKAACEECEGGGQVDAE
                     RVVKVTVPGGVDSGQSLRLSGRGQEGVRGGPAGDLYVTVHIEPDPDFQREGFDLVHRL
                     SVTYPQAALGAKVDIPALEEDEDAIQTIKVPSGTQAGETLRVRGAGVPRLHGRGRGDL
                     ICVVEVEVPKRLSREQKRLLKELDRTFDES"
     gene            complement(11609..13075)
                     /gene="nadA"
                     /locus_tag="VFG22_14755"
     CDS             complement(11609..13075)
                     /gene="nadA"
                     /locus_tag="VFG22_14755"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF014498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HET6417554.1"
                     /translation="MTLPVFPSLRITADALIPEGAFAEAQATFLKPNPELVSRLDALL
                     REKNTGVVAHFYMDPELQGVLSACSWPHVHVSDSLAMADRAIAMAEQGVSTVAVLGVD
                     FMSENVRAMLDASGHEQIPVYRVATDPIGCSLAESAEAAAYGAYLMEASETPHSLHVV
                     YINTSLVTKAKAQAIVPTITCTSSNVVRTVLQAAAQIPDVHIWFGPDTYMGENLAQMF
                     RSLAEMPDAQIQQLHPDHDQASVRSLLARFHYFEQGTCIVHHMFGREVVDLVRAHYSD
                     VFVTAHLEVPGEMFELGLQKQQDGNGVVGSTSNILAFVLAKVDEAIAKGEGANLRFIL
                     GTEAGMITPIVRQVQAKLRDYASEGGPELSAEIIFPVASEAIAQDAQSGLGIVPGVAG
                     GEGCSTAGGCATCPYMKMNSLHALLGLLERVDAEPRSNLVPFEPRKYAQEIRGRTAAD
                     LGGEPILHMRAFQRSGELPEALVSDVLQRRPAPASSLV"
     gene            13125..14279
                     /gene="hemW"
                     /locus_tag="VFG22_14760"
     CDS             13125..14279
                     /gene="hemW"
                     /locus_tag="VFG22_14760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HET6417555.1"
                     /translation="MSSEPTETSVYIHFPWCTRKCPYCDFATEPIRSSALPHQEYADA
                     VLRELEARRSDLKGRKLKSVFFGGGTPSLWGPHALGRTLRGIIGAFDDLDEALEVTVE
                     CNPSSLHQENAALLREAGVNRLSLGLQSLRDLHLRFLGRLHDSERALASLGDACKEMP
                     RVSADLMFGMPGQTLEELAEDISRIAEIGVRHVSAYALTIEEKTQFGSLHRAGKLEVA
                     PEDQYADLFEHVEDRFSDLGWTHYEVSNYAAEGEESRHNLHYWRGGAYLGLGAAAVGC
                     LDQGQGRAERWRNDPNPQRYLRQSTLESEVEMLGPKEIVREALMLGLRTIDGMNVAST
                     ERRAGCDPKTGREREIERAIDQGKLLQVGDWLRVPQKHWLKLDGIVRDLF"
     gene            14291..14947
                     /locus_tag="VFG22_14765"
     CDS             14291..14947
                     /locus_tag="VFG22_14765"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6417556.1"
                     /translation="MKLDFEHVSVTRLAKSYGPTLALAGVDLGFDAGTVTAIQGSNGS
                     GKSTLLWLLAMLAYPTRGEIRYGVHGVSRSDGLRGRIGFVAHQLQLYPGLSGRENLVL
                     AAKLQSIPYAEKRADDLCEALTLGEYWNRPVRTYSRGQAQRVSIARALLHEPRLLLMD
                     EPATGLDVKSAASLVELVEREKQQGSIVIIVTHEAKFAELVADRVLYMDRGRIVEPSA
                     "
     gene            14944..15624
                     /locus_tag="VFG22_14770"
     CDS             14944..15624
                     /locus_tag="VFG22_14770"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HET6417557.1"
                     /translation="MNTLRAALLVALKDLQLELRTKEILTSTGLFAVLVVTLGSLAFY
                     TDPVSSPNVAPGVLWISILFCGILLIGRSWSLERENDAVFALLLAPIPRASIYIGKTI
                     SALAMLFAIELLLVPLCLVFFQIEPKGVVLPLLALVFLGSLGFVSTASLFSAVGIRTR
                     ARDLMLSVLVFPLVAPALLASAVATRELFAGASMSEIIGWMRILTAFDLLFVGFGVWV
                     SRHLLAET"
     gene            complement(15628..16236)
                     /locus_tag="VFG22_14775"
     CDS             complement(15628..16236)
                     /locus_tag="VFG22_14775"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET6417558.1"
                     /translation="MPKREVIATFIVAAIAFPLMFIFTGAIVDGRARAREAPFRALLG
                     NSRFEELMEGQGGVPHYLGASLLAPDFSLPTQGGGEWKMSEQRGKVVVMNFWTVTCRP
                     CIQEMPTIELLAEITEDWGDVEIVAVSTDRSWEEAETIIPRTSRITSLLDADRQVVTG
                     MFGTKLFPETWIIDADGVVRFRFDGSLDWSDPVALDLIRAYR"
     gene            complement(16246..16740)
                     /locus_tag="VFG22_14780"
     CDS             complement(16246..16740)
                     /locus_tag="VFG22_14780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417559.1"
                     /translation="MDPAQFLKGRTRETEIRRDASGRWFNGEDRIEHPKLARSFDSWV
                     DRAEDGRFCLSNSINWAYVEIEGPAYFVKDVEANPEGIELHLSGDLRESLDPSSLAQG
                     PEGALWCVVRDGRCPARFDNHAVSKMSGSIGEDDDGVFVLIGGQKVRPPELSDPLSLG
                     LGKR"
     gene            16769..18160
                     /locus_tag="VFG22_14785"
     CDS             16769..18160
                     /locus_tag="VFG22_14785"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HET6417560.1"
                     /translation="MVRKSRKLVVVSNRGPYRHEAARGKERWVRSAGGLVAALDPVLQ
                     MRGGVWVSAKPAKDFDSVTVPSPDLAYDLAHISLKRGDQRGFYEGISNAVLWPLLHGF
                     EPTIQVGEAPWSSYLGANQAFADTTLATSGPSDLIWIQDYHLMLVPAMIRAKRSKARI
                     GWFCHIPWPPPDTFGILPWREEILEGLLGADVLGFHLPEYAEHFRACVERFTTYRVSR
                     GAIRYRGRSVKTIAAPIGIPVDELQALAIDPDVGREVERIRQLMVNRKLILGVDRLDY
                     TKGIPERLAAFERLLARDKDARKRYALIQIMVPSRTDVQAYADLKQEIDRMVGDINGR
                     YAETGRVPIHYLYRNLSQRALFAHYRAADVALVTPLRDGMNLVAHEYAAARTDEDGVL
                     ILSEFAGAAKHLKGAMLVNPYDVDSTTNAIHRALTMKPAEKRKRMRSLREEVMRLDVH
                     LWADRYIAALEGK"
     gene            18157..>18595
                     /locus_tag="VFG22_14790"
     CDS             18157..>18595
                     /locus_tag="VFG22_14790"
                     /inference="COORDINATES: protein motif:HMM:NF014418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HET6417561.1"
                     /translation="MTRIDSLIERLLRLERPMLLAFDVDGTLSPIVRDPDLARIPEET
                     LRTIAELVESPMTEIALITGRDLSSLGRMEQLDGIWRAVEHGGLVLAPGDRPAPRQLR
                     AEQEAALDRFRHWVDAHAEGAFIEYKPQAIAVHVRAIAETDPER"
BASE COUNT         3512 a         5946 c         5799 g         3338 t
ORIGIN      
        1 ttcagctcgt aagcgcggac tccgcatgac tcgaatcgat cagggtcagc gatacgacga
       61 aaaccggccc cgcaaagcgg agccgttgtc gccagatgga gccccgtaga acggggcgga
      121 gcgggaaatg agattcgaac tcacgacttc aaccttggca accctttcgg ggccctgaac
      181 accattcggg aatttcaaca acatcaacga catagtgtcc cgtggcgttg cgtgccgtcc
      241 cgaggtgtcc cgtgggaagt ggaaggttgg gtggatcagc cacccccgct ggagcctgtg
      301 ccttggctcc tcgccagcgg gaagaagtcg atgacgttcc cggtgttgac ggggaacgtc
      361 tggccggagc tcgccccggt attgggatcg aaccgctaga cggtgaaccg gtcgatgacg
      421 tagaggagcc cgtcctccgg ccctaccgcg aagtcgccga tgctttgctc gcccgctttg
      481 atgaacggca cgatgagctc cgcgtctccg gtcgacgggt cgaatcgact tcggatcgat
      541 ggccccggca gcggccgccg catccgaatc ggtgccgccg gcgagcagcg tctggagggc
      601 gcgttcatgc cgtggcgaca gcctcggttg ggtcggcgcg cactggtcgg cagtgtctcg
      661 tggctgcggc aaactgcggc gtcttgtagt catctcgcct ggaccaaggc cttccagcac
      721 cctgggcgca tcagcagcga ggaacgtctt gacccgatcg gcgaggcgag agagaacagc
      781 tgcggcagtc agtagcaatg gaacaaccag acgtagaccc agcgatcctg cgtgctgtgg
      841 gcgacgtggg tcggcccgag cgcccctacg aggacctggc aagcctcagg catatcccag
      901 gcaaggacgg ggccatcacg ggcgtgatga acctttgggg ctggatgagg catgggcgcg
      961 cgcacttgga ggcgcaggac aagaagtacg gacccatcta tcgcattcga gttggaccga
     1021 cggtctacat tgcggtcaat gatccgacca ttggggcaca gatccttcgc aacgcagacg
     1081 gtgcatggtc ggcagcgctc ggcaacctgg tactatacgg cgggattcca cctgacggcc
     1141 cgatggatgc gcccgtcacc ctggatttcg agccgcatcg agacgttcgg cgattgctac
     1201 aacccgcctt caaggccgac gcgctcgcga gctacgtaga aaccgcgaac cggcacctcg
     1261 cgacggcgac cgaccgatgg gtgaccgcga gggaggtcaa gttcaagcca gaagcgcgag
     1321 gactgttcgc tcgcgttgcc gccgacgtat ttcttggcgt cgaggaccaa gagcgggcgg
     1381 aagagctgga caaagcgatg agcgacttct ggcacggcat caccgcagtc gtcaggaacg
     1441 aatggctgag cccgacatgg cgggccgcac gccgccagta ccgcggcttg atcgagcgtt
     1501 tccgcgcaca ggtagccgct agacgagcaa cggatggagt ggacctattc acgcggctct
     1561 gtcagacgaa cgaaaaggtc gattggctcg acgacgaggg attggtgcga gtgctcacgg
     1621 gcttgatggc agcggcgttc gacacgactt cggcggccgt gacgagtatg gcgtacgtgc
     1681 tcgcgaccga cccagagtgg caggaatcgc tgcgggccga ggcgatagac ctcgaaaccg
     1741 agaccgtgac gtacgagcag atcaaggatc ttaaccagct cgattgggtc tggaaggaaa
     1801 ccctgcggtt ctacccggtc gttccgaccg tttggcggct tgcgctacgc gacgtagacg
     1861 ttggtgacta tacgattccg gctggcggtt tgatcatggt cgggatcagc atggcccttc
     1921 gtgacccgtc gagttggaag tacccgaacc gattcgatcc cgagcgattc tccccccctc
     1981 gccaggaaga caagcacaac cgcgcagcgt tcttgccgtt cggtgcgggg caccatgcct
     2041 gcatcggatc gcagctggct gggctcgaag ccaaggcgtt ctggtaccag ttcctacgca
     2101 aggcgcgcat tcgcctcgct cgtccatacg gagcacgcca cgggtatggc ccactgggca
     2161 tggtgtcggg cgatgtgact ctggttgtgg agccggtgta ggcagcactc tcgaacaact
     2221 tcgaccttgg cagccctttc cgacgcctga gcaccgcccc gaacaggccg cgcggcctgt
     2281 tgtaaacacc ctgaattggg cacccgctga attgaaaaag gctccgcgaa aacggagcct
     2341 tctggagccc cgtagaacgg ggcggagcgg gaaatgagat tcgaacttac gacttcaacc
     2401 ttggcaatat tcgcctagct cccaacacca ttcgggaaat tcaacaacat caaccacaaa
     2461 gtgtcccgtg gcgttgcgtg ccgtcccacg gtgtcccctg gaaagtggaa ggttcagtgg
     2521 aaggtgtgta ggtacctcga gctccatttc tccagctcaa agccagccgc ataaggctgc
     2581 gggagcgggc gtttgttttt gctaggctcg taccgcacgg gctgccgggg aggcatcgac
     2641 gctgcactct ggacgattca ggacgggccc cacgtcccgt tcttctactc gcccggagga
     2701 gaggagccgg ccttcacgga cctggtcacg gactggctcc tcgaacacag ccgctagcct
     2761 gccggcgcgg gcttccgcca ctcgcggtag tcactcagag cgagcgcagg tacgcgatca
     2821 gggcggcttt ttcagtctcc gagagcccgt cgcaggtaaa catgtgacca cgatcgctgt
     2881 acggcgattc ggtgatcgtg ggtcgcatgc cgttcacgca gaagagatct tcgagcgagt
     2941 cgacggagcc gttgtgaagc agtcgcttgg ccgaccaaac cccggtgagc cgttgcgcgc
     3001 ggaggccgtg cgtcagtgca tcgcgcccga gcaagtcgac gtcgcaacag ggttggccat
     3061 cgagatcgcg gtcgagccag tacatcatgg cggtgtcgga gccgatctcg tcgaaggtga
     3121 agacggtgcg gctcgaaccg cgggcgccgt cgtgacagtc gatgcagtct gcggtgccaa
     3181 aaagctgctg cccttcggcc acgcgaaccg cgtcggacgg ctccgggttg ctgggcgcgc
     3241 gcaacgagtg gagatagtca tccagcgggc cgagccgttc tgcgtcccat gcgtctaggt
     3301 cgccgtcgcc aagaatgacg aaaccctcga gaaaattcgc gacgctggcg gtcacacccg
     3361 tgtgagccag cagtgcccca tccatctcgt aggcagcaac gtctgcctcg tcggcgatcc
     3421 cccagagcga catgatcttg ctgacggtgt gaacctcgtc gtcgagcggc aacggtgtga
     3481 tcacgaaatc catcgtgccc gacttccagc ctgcgtaggc ggcctgagtc tccgcgctga
     3541 actccggaat gctcgcccca gcaccgatga gtggcgcaag cgatgcgatg agcccgtcaa
     3601 cgatgctggg gtcatcctcg atggcttgga agtacggcgc gagcttcgcc tcggcggcgg
     3661 ggtctaggtc ctgttgaagg atggtggcag tcggcaagac cgcgatggcg aggttgagtt
     3721 cgccgtactc gaaatcgtga ttgggcgcgc ccatcacata acggccgtcc ggcagctggc
     3781 cgaagtgaca gctcgcgcag gtgaacgcca ggctcgggac gccctcagcg aagtccttgc
     3841 cgggcccgaa cccaagtggc agcccgtccg ccgaagtcgg gtcttcgatc atgccgacgc
     3901 cagttgcacc tgctccgatg aggtccggaa acaacgccat gttggtttcg agcagcacgg
     3961 tgggcacgcc gaccgtgtcg aagccttcgt agaagaacat ttccttgccg cacagcagtc
     4021 gcgcacggcg tgtgtcggtc ccgtcggcaa cggcatccca atacgcttcg cacgcaccgt
     4081 gcagcgcccc atcctccaag acttccatga gatcggcgga gacattggta agcccctcac
     4141 tcgtgtcggc ttgaggggca aaggggtcgg ccggcgcagt gccgccgctg tcgttgctgc
     4201 tgcaagccgc agcgctgagc gcaacgaggg cgctgggcaa aagcaagaat cgattcatcc
     4261 caggatctcc aaagttgagc gtggtgcgcg tagcacggct agccgggaat cgaaaccgcc
     4321 cgacgctgcc cagaccagaa ccagcgtgtg agcggcggcg tgcgtttcgg cttcacctcg
     4381 agtgcagaag atctgaccga ggttgagcag aagtctgatt gtcacgcaac cggattggtc
     4441 ctccgaccca taagcgcagc ctccgcgtcg ctcagcctag tcgtcccgac cccccaaacg
     4501 aaaaacggcc ccgcagtccg gagccgtcat cgccatttgg agcgggaaat gagattcgaa
     4561 ctcacgactt caaccttggc aaggttgcac tctaccgctg agttattccc gcaaaaggac
     4621 gcctttggta gcgcggccag gggcagccgt caagctcttg gcgccctgcc gaaagccctc
     4681 cgaaaacccg aaatagcccg ggttttcact cgtagcgcag cccatccaca ggcagcatcc
     4741 gggcagccca ccaggacggg gcgatcgtcg agacggtgca gatcagaagg gcgatgccga
     4801 tggtgaccag gaataccgtg gggttgagca cgaccggcaa gtggtcgatg agatagacct
     4861 tggggtcgag cggaaagcgt atcttgtcga gataggcgac gagcccaccg ccaacgagta
     4921 ccccggccaa ggttccgatc acgccgacga ccacgccttg caccatgaag atgccgagga
     4981 cggtgccatc ggtcgcgccc atcgctttca ggatcgcgat ctcgcgcttc ttctcgatga
     5041 cgatcatgat caaggtcgcg atcacgttga aggccgcgac gaagatgatg gtggcgatga
     5101 ccaaggtgag cgcgatcttc tgcacttcga gcgcggtgaa gaggttgcgg ttgagctctg
     5161 accaatcgag cgagtgaaac gccccgccga ggtcttgctc gatgcggcgc gcgagctcgt
     5221 acgaggtctc caggtcgtgc acgcggagct cgacgcccgt cacgttgtcg ccctcgtcga
     5281 agagccgctg tgcctcgaat agatctgcgt agacgaggcc cgagtcgtac tcctgaaagc
     5341 ctgcttgaaa gacgccgatg acccggaact ccctcgagcg ggtcgttgag ccactcggct
     5401 cggacaaggc gagcccgaga gcggccatgg gcgagatcaa gcgcacccga tcgccgagct
     5461 cgagctcgag gtcttcggcg agcgtcttcc cgacgatgat ccccggcaag ctgtcgatcg
     5521 gttcttgggc tgcaccgtac gagaggccgg attcccaggc ggggtcatcc ggtaacgcca
     5581 gcgcttcctc gtcgagctct ccaagcatcg tttcgatctc gaccggcgcg gccacggcgc
     5641 cgattcgcgg tcgacgggcg cgctcggcgg cttcgcgctc ggccgccctc cgttccttct
     5701 cctcttccca gaacgtcttt cgttggaccg gcgcctgctc agccttggga ctcgggggct
     5761 tggtgctctc ggtcttccca tcgcgcaggt cgcgtagcca ttgccagtcc tggtcttctg
     5821 ccggctcaac ggcgcgggtc ggggtcgagc cgggccggcg aagaccttcg agtccacccg
     5881 caaccatctg attgggaagg tcgagcaccg tacggacccc ttcgggggtc acgcctttga
     5941 cgaggacgac acttcggcgc tcgcccttgg tgagcatcat ctcgttgatc gagaagggcg
     6001 cgacgccggc tacttccggc atctcaccca cggtcgagag gacgtcacgg tagtcgcgaa
     6061 actcgcggcc gtacttcagg accaggacat gcgcgttgac gcctaagacc ttgtcgcgga
     6121 aggctttttc gaaacccgtc gtgatcgaga gcactgccag tagggccgct acgccgagcg
     6181 cgacgccgat gatcgagatc agggtgatca tcgaaaccgt tgcccgcttc ttcgaccgca
     6241 aatagcgaac accgatctgg aggctgtaac tcactccgct ggggcttaac acggcgccgc
     6301 cggggcggcg aacacgcgcc ctcgctcctg aacttgcccg gcaggtgcgg actcactagg
     6361 gttcgtcaga tgaagcggat ctggcccttc gcgatgagca tcgggttgct ggtggcctgc
     6421 catcagcagc agaacgtgcg gcgcgaagtc tggccgtcgg caaaccagcc gaagcccccc
     6481 gcggccgccg ccgaggctcc gactccgccc cccaaagtcc tcgacccgat cgtcgtcgtg
     6541 ctcccggaga agtctccggt gatgcaggtc cgcaaacaac tcgacaaggg caaccccggc
     6601 aaggcacgag aagttgccga ggcagcgctt ccggcagcaa gcgcacgcga caaggggcgc
     6661 cttcactggc tggccgccaa ggcagcgctc gacgaagggt acagcagcat tgcgctggcc
     6721 cacctcgaca cgctcagcaa cttcaaccat ccgctggcgc gctgggcgaa gctacgccgc
     6781 gcttcgctct tggagcgaac cgacccggtg accgccgctc agatcgccgc gtccttgacc
     6841 gacgactggg ccggcagcaa acgggcgcga acgatcgaag tgcgcagcgg gggcagcgcc
     6901 gacgtgtcgg acgcccatct cgagatacgt ctcgacggca cactgcccgc gaagacatcg
     6961 gaagcgcctg ccccccagca ggacgtccgt ccgcgcatgc agcaaagcct cgacaaggcc
     7021 caggccctct acgactcgaa gcgctacaaa gaggccgagc ggctctacgg aaagctcgtc
     7081 aaacatctca agacaggaag cagtgcgtgg tgcgacgcac gctacaagca aggccgcgcg
     7141 cttctgcaag accgcgagcg cacgcgtggc gcaccggtga tgcgcgaggt ggcggacaag
     7201 tgcggcaggg ccgacccaga aacccggacc tgggcacgtt accactcggc gcgtgcctac
     7261 ggacgcatcg gcgattggaa cgaatcgatt cggcagtacg ccaagctcga agccgaggac
     7321 ccggcgcaca gcttagctga cgacgccagc taccgggcgg cgctcgccga gctcgaaaac
     7381 ggcaggccca aggctgcgat cgcacgcctg tcctcgatcc cgaggcgcta tcccgaaggc
     7441 gacatgtggc cccgggccct gttcaagctt ggctgggtgc atctgcgcga cggtgattac
     7501 gagaacgcct acgaacgatt cgatgagctg gtggctcgcg gtggcgacga acgcgaggaa
     7561 ggcataaccg gccgcgcgaa ctactggcgc gcacgcactc tcttcatgat gggacggcgc
     7621 atcgacgcga tcgccgccta cgaagagatc gttcagcgct ggccgctccg gtactatgcg
     7681 caacaggcgc tctggcgcat tcaggagatc gttccggccc aggccgaggc tctgctcgta
     7741 cagatgcgcg cgggagacga gcggacgcgc atggtcttcg cctggcgcga tgagttcgac
     7801 gatccggcgt tcgatcggat gctggagctc ttgcgggtga acgagatcac gtatgcatcg
     7861 tccgagatgt ccacgcttca catgctcggc aagaacgtgg atccggagat ggccgtggtg
     7921 ggcgccgtgc tgcttcagca tgcgggcgct gagaccaagc tcagccgggt cgtccgaacg
     7981 catttcgaag acttcgaagg cctgcttccc aagggcgaag gaaaactgat ctgggaagcg
     8041 acctacccac aagcgttctc accgctgatc gaagaaatcg caagccatga aggcatcccg
     8101 gcctcgttcg tgcgcgcgat cgcacgcgaa gagagctcgt tcgacccgga ggccgtgtcc
     8161 tgggccaagg cctacgggct ggttcagctg atcatgccga cggcaaagcg cttcgccaat
     8221 gaggtcggcg agaaggccac ccctcggacg ctcaagaaac ccgaggtgaa tctccggatc
     8281 ggcgcgcgct acatggcctg gctctgggag cgtctcggcg aaaaccccgc gctagtgcca
     8341 tcggcttaca atgccggcga gggcgccgtt cgccgctggc tcgaagaaga cgctgcccgc
     8401 cctctcgacg tgttcatcga ggaaatcccc tacgacgaaa cacgacgcta cacgcgcagg
     8461 gtcttgcaaa cctacggcgt ctatcagtgg ctggctgacg aaaagctgcc cgcgctgcct
     8521 gcgaagctcc catcgacgaa cgcgccgcag cgcttcagcg cgcttcgctg agccctcagg
     8581 cgacgacctt cggcagcagt gcgccgaact cgatcgcctt gttgatgtcg ccatccacgc
     8641 gcagcttgcc ctgaacgaac gcgatctgcg ggtcgagctt gccacgcaag atctcagcga
     8701 gatgtgcgtc acggatggtg agcgtcgcgt ccgtgggacg accgttgccc tcggtgacgc
     8761 tacgttcctg cagatcgatc atccagctct ggtcggctgc gccggtgacg cgaagctgaa
     8821 acgtgccttc accgagggag cgttccgctg gcgccgcggc aagcgtcgat ctcatctgct
     8881 cgacgaaggc agccgtcatc tcggccacag ccttggattg cgcccggctg gcttcgtctt
     8941 ccggtttccg gagcgccagc actgccgctt cgagctcgtt ggcagattcg cgaagatagc
     9001 gggtgaagag gtccaggtcg ggcatgacct tcgggcagga cgttggactg atcgaaagca
     9061 ctccgttgta gctcagcacc gtgatcagca ggcccatccc gtcgacgagc ggcgccattc
     9121 ccatcgtcgc gaggagcttg tgccccgcca tgtacatgtc catctgcggc ccgggcacgt
     9181 tggtgatgac gacgttgaac accgggttgt gcatgtccga gacacgaaac cgcgagtaga
     9241 gccggagcac tcggttcgcc aagccgaagg gaatgaaatc ccagagatcg acgaacgccc
     9301 gcgtcgcacc ggccttctcg aaagccttgc cgcccttgcc gtgctcctga atcaccttca
     9361 ggcgctcgac cggatcggcc acgtcggtcg cgatgtcgag gaacatcgcg gagaccttgt
     9421 ttcccatctt tccatgctcg tctttgccac gagtcgaaac ggggatcatc gcgatcagcg
     9481 gcttgtcggg gagcgcgtcc ttctcttcga gataccggcg gagcgccccg gcacagatcg
     9541 ccacgacgac gtcgttcaag gtgcagcccg tgatcttgcg aatctccttc acgcgatcga
     9601 gctcgagaag cgccgtgttc caaattcgct gtcccgtcac cctttggttg atcggcgtgt
     9661 gaggcgcagt catcggaacg ggaggcgcgt cgacgccctt gcttttcacc ttggccgcgg
     9721 cgcgcagggc ctcgttgatc tcgcctgcga ctcgcttgat ctccgtcggc ttgcccatga
     9781 tcttccgagc ggtgtgtgaa agcacttgga gctcgtttgg cattggaggg atcgcgcgag
     9841 gcggaggggg gatgaacctg cgcggctcct tcgtcatgtc gagcagcaca ctcaacatgt
     9901 ccgatcccga aaccccatcg atggccgagt ggtgcacctt gttgatggtg gctatcgacc
     9961 cgggcggcac ctgcggaatc tggtccagcc cttcgacgaa ggtcatttcc caaagaggcc
    10021 tcgaacggtc gagcggcacg ctgaagatcc gagccgcgag ccgacgaagc tctttccagc
    10081 ttccggggct cgggagggca acgtgctgca agtgcatgtc taggttgaag tctgggtcct
    10141 cgacccagta cggctttccg atcccgaaag gcaccatcgc gagtcgttgc cggagccgcg
    10201 ggacgagatg aacgcgcgag gccatcgtct cgcgaaactc gtcgaaggtc atggagcctt
    10261 cgaaaactag cacgctgccg acatgcatcg gttggtgcgg gtcctcgaga tagaggaaag
    10321 aggaatcgag tccggtaaga ggctgcatca tgggtcttcc tagggcactc cccaaagtag
    10381 acgactagcc tacacccaga ggcgtacgga tctccagcgc tactcaggac tcgtcaaaag
    10441 tacggtcgag ctccttcaag agccgctttt gctcgcggct gagccgcttg ggcacctcga
    10501 cttcgaccac acagatcagg tcaccacgcc cgcgaccgtg cagtcggggt acgccagcgc
    10561 cccgaactcg caacgtttcg cccgcttgcg ttcctgaggg caccttgatc gtttggatgg
    10621 cgtcttcgtc ctcttcgagc gccgggatgt cgaccttggc cccgagcgca gcctgtgggt
    10681 aggtcaccga aagccgatgc accaggtcga agccctctcg ctgaaagtcc ggatcgggct
    10741 cgatgtgaac cgtcacgtag aggtcgcctg cgggtccacc tcgcacgcct tcctgacccc
    10801 ggccgctcaa gcggaggctc tgaccactgt cgaccccgcc aggcacagtt accttcacga
    10861 cgcgttcggc gtcgacttgg cccccgccct cgcattcctc gcaagcagct ttggctcgca
    10921 caccccggcc ctgacatgtt gggcagcccg tcgtcatcac gaatgcgcct tgcgcgcgcg
    10981 cgacctggcc tctgcctccg caggttgcac agccttcgag ctctccgtcc ttcgcgccgg
    11041 ttccgtgaca ggcctcgcag ggcgacggat gcgaaaggtc gatctcgcgg ctgctgccaa
    11101 atgccgcttc gcgcagcgac aggctcaagt ccgtgcgaat gtcggcgccg cgttgagggc
    11161 cgttcggatc acgccgccgt cggccaaagc caccgaagcc aaaacctccg ctgcctccaa
    11221 agaggtcacc aaagatgtct tggaactggc tgaagacgtc accgatgtct gcaaagcccg
    11281 gtccgccacc gccgctcagg ccggcgtgtc cgaagcgatc gtacacatcg cgcttctgag
    11341 ggtcgctcaa gacagaatag gcctcggcgc aaactttgaa cttctcttcg gcttccggat
    11401 tgtctgggtt gcggtccgga tgaagctcga gggccttctt cctgtacgcc ttcttgagct
    11461 cggtcacggt gacttcgcgg gagacttcga ggacttcgta gtagtcaggc ttcgacatgg
    11521 gctcttaggg ctgaacggcg cgtgcaacat aagtacgccg tatttccggt caaggtccgg
    11581 atttggccga gaaactgtgt tttctccatc aaactaggct gctcgccggt gcgggccggc
    11641 gctgaagaac atcgctcacg agggcctcgg gaagctcgcc cgaacgctga aacgcgcgca
    11701 tgtgcaggat cggctcgccc ccgagatcgg ccgccgttcg accccgaatc tcctgagcgt
    11761 acttgcgcgg ctcgaaggga accagattgg atcgaggctc ggcgtcgacc cgttcgagca
    11821 ggcctagcaa ggcgtggagc gagttcattt tcatatacgg acaggtcgca cagccgcccg
    11881 ctgtcgagca accttcgcca ccggcgacac ccggcacgat tccgagcccg ctttgcgcgt
    11941 cctgagcgat cgcctcgctt gccaccggaa agatgatctc cgcggagagc tcgggaccgc
    12001 cttccgaggc gtagtcccga agctttgcct gcacctggcg aacgatcggc gtaatcatcc
    12061 cggcctccgt cccgagaatg aatcgaaggt tggccccttc acccttggcg atcgcttcgt
    12121 cgaccttggc gagcacgaag gccagaatgt tcgaggtcga gccgacgact ccgttgccgt
    12181 cttgctgttt ctgcaaaccg agctcgaaca tctcgccagg gacctcgagg tgggcggtga
    12241 cgaacacatc gctgtagtgt gcgcgcacca agtcgaccac ttcgcggccg aacatgtggt
    12301 gaacgatgca agttccctgc tcgaagtagt gaaatcgcgc cagaagcgag cgaacggagg
    12361 cctgatcgtg atcgggatgc agttgctgaa tctgcgcgtc cggcatctcc gcgaggctcc
    12421 ggaacatctg cgcgaggttc tcccccatgt acgtatcggg cccaaaccaa atgtgcacgt
    12481 ccggaatctg cgccgcagcc tgcagaacag tccgcaccac gttcgaggac gtgcaggtaa
    12541 tggttggcac gatcgcttgc gccttcgcct tggtcacgag gctcgtgttg atgtaaacga
    12601 cgtgaagcga atggggcgtt tcggacgctt ccatcaagta ggctccgtag gccgcggcct
    12661 cggcggactc ggccagagaa caaccgatcg gatcggtggc gactcgatac acggggatct
    12721 gctcgtgtcc cgatgcgtcg agcatggctc gaacgttctc gctcatgaag tcgacaccga
    12781 ggacggcgac agtgctcacg ccttgctcgg ccatcgcgat cgcgcggtca gccatcgcca
    12841 gagagtccga gacgtgaacg tgcggccagc tgcaagcaga gagcacccct tgaagctccg
    12901 gatccatgta gaagtgagcg acgacgcccg tgttcttttc tcggaggagc gcgtcgagcc
    12961 gcgagacgag ctcggggttc ggcttcaaga aggtcgcttg cgcctcggca aacgcgccct
    13021 cggggatgag ggcatcggcc gtaattcgta gtgatggaaa caccggcaag gtcatctgcg
    13081 accagctcca aaaagcggag tataccttct gcccggcgca ccgcatgtca agcgaaccca
    13141 cagagacttc ggtctacatc cactttccat ggtgcactcg caagtgtcca tattgcgatt
    13201 ttgcgaccga gccgattcgc tcgagcgcct tgccccacca agaatacgcg gacgcagtgc
    13261 tacgcgagct cgaagcgcgc cgttcagatc tgaaagggcg caagctcaag tccgtgttct
    13321 tcggcggcgg cacgccatct ctctggggcc ctcatgcgct gggtcggacc ctcagaggca
    13381 tcatcggcgc gttcgacgat ctcgacgagg cgctcgaggt cacggtcgaa tgcaatccga
    13441 gctcactcca tcaggaaaac gccgcgctct tgcgagaggc cggagtcaac cgtctctcac
    13501 tcgggcttca atcgttgcgc gacctgcatt tgcgcttcct cggacgctta cacgactcgg
    13561 agcgcgcgct ggcgtcactt ggagacgcct gcaaagagat gccgcgcgtg agcgcggacc
    13621 tgatgtttgg aatgcccgga cagacgctcg aagagcttgc cgaggacatc tcgcggattg
    13681 ccgagatcgg cgttcgtcac gtctcggcat acgccctcac catcgaggag aagacgcagt
    13741 tcggctcact tcatcgcgcc ggcaagctcg aggtcgcgcc cgaagatcaa tacgcggatc
    13801 tcttcgagca cgtagaggat cgcttctccg atctcggctg gacgcactac gaggtctcca
    13861 actacgcggc cgaaggcgaa gagtctcgac acaatcttca ctactggcgc ggcggcgcat
    13921 acctcggact cggcgctgcc gcggtcggtt gcctcgatca gggtcaaggg cgcgcggaac
    13981 gctggcgcaa cgatccgaat ccgcagcgct atcttcgcca gtcgaccctc gagtcggagg
    14041 tggagatgct cggccctaag gagatcgtgc gcgaggccct gatgctcggc cttcgaacga
    14101 tcgacgggat gaacgtcgct tcaactgagc ggagagccgg ctgcgacccg aaaaccgggc
    14161 gtgagcgcga gatcgaacgc gccatcgacc aaggcaagct cctgcaagtg ggagactggc
    14221 tccgcgttcc gcagaaacac tggctgaaac ttgacggcat cgtgcgagac ttgttctaga
    14281 gtgcgaaccg gtgaagctcg attttgaaca cgtgtcggtg actcggctag ccaagtccta
    14341 cggaccgacc ttggcactgg ccggcgtgga cctcgggttc gatgccggaa ccgtgaccgc
    14401 gattcagggc tcgaacggct cgggcaagtc cacgctcctc tggctgctcg cgatgctcgc
    14461 gtacccaacc cgaggcgaaa ttcgatacgg cgttcatggc gtctcgagat cggacgggtt
    14521 gcgcggccgc atcggcttcg tcgcacatca actccaactc taccccggcc tcagcggacg
    14581 ggagaacctg gtgctcgccg ccaaattgca gagcattccg tatgccgaga agcgcgccga
    14641 tgacctctgc gaggcactca cgctcggcga gtattggaac cgcccagtac gcacctattc
    14701 ccgtggccag gcccaacgcg tctcgatcgc cagggccctt cttcacgaac ctcgtctcct
    14761 tctgatggac gagcccgcga ccggcctcga cgtcaaatcc gccgcctcgc tggtcgagct
    14821 ggtcgaaaga gaaaagcagc aggggtccat cgtcatcatc gtgacccatg aagcgaagtt
    14881 cgcagagctc gtggcagacc gtgtcctcta catggaccgc ggccgaatcg tggagccgag
    14941 cgcatgaaca cactccgcgc cgccctgctg gtcgccctca aagacttgca gctcgagctc
    15001 cgcaccaagg agatcctcac ctcgaccggg ctctttgccg tgctggtcgt aacgctcggc
    15061 tcactcgcgt tctacaccga cccggtgagc tcccctaacg tcgctcctgg cgtgctctgg
    15121 atctcgatcc tgttctgcgg gattctcctc atcggccgaa gctggtcgct cgagcgcgag
    15181 aacgacgcgg tcttcgcgct gctactggca ccgatcccga gggcgtcgat ctacattggc
    15241 aagacgatca gcgcgctcgc gatgctgttt gcgatcgagc tgctgctcgt ccccctatgt
    15301 ctggtcttct ttcagatcga gcccaagggc gtggtcctgc cgctgcttgc cctcgtgttc
    15361 ctcggctcgc tcggcttcgt ttccaccgcc tcgctctttt ccgccgtagg catccgcacg
    15421 cgcgctcgcg atctgatgct gagcgtgctc gtatttcctt tggtcgcccc ggcccttctt
    15481 gcctcggcgg tcgcgacacg cgagcttttc gctggcgcca gcatgagcga gatcatcggt
    15541 tggatgcgga ttctcacagc cttcgatcta ttgttcgttg gctttggggt ctgggtttcg
    15601 cgccatctcc tcgcggagac ctgagcctca gcgatatgcg cgaatcagat cgagcgcgac
    15661 tggatcggac cagtcgagcg agccgtcgaa tcgaaagcgg acaacgccat ccgcatcgat
    15721 gatccaggtc tcgggaaaga gcttggtgcc gaacatgccg gtcaccacct ggcggtccgc
    15781 gtccagcaag ctcgtgatcc gagaggttcg cggaatgatc gtctcggctt cttcccagct
    15841 tcgatcggtc gagacggcaa cgatctcgac atcgccccag tcctcggtga tctcggccag
    15901 gagctcgatg gtcggcatct cttgaatgca gggccggcag gtgaccgtcc aaaagttcat
    15961 cacgacgacc ttgccgcgct gttcggacat cttccattca ccgccgccct gcgtcggaag
    16021 cgaaaagtcc ggcgcgagca gcgaagcccc gaggtagtga ggcaccccgc cctggccctc
    16081 catgagctcc tcgaagcgtg aattgccgag gagcgcgcga aacggagcct cacgggcgcg
    16141 cgcacggccg tcgacaatgg cgcctgtgaa gatgaacatc aaaggaaacg cgatggcggc
    16201 cacgatgaat gtggcgatga cttctcgttt gggcactccg ggggcttagc gctttccgag
    16261 gccaagtgaa agtggatcgc tcagctcggg cggacgcacc ttttgcccgc cgatcaggac
    16321 gaaaacgccg tcgtcatcct cgccgatgga gccgctcatc ttgctcacgg cgtggttgtc
    16381 gaagcgcgcc gggcatcgac catcgcgaac cacgcaccag agcgccccct ctggcccttg
    16441 agcaagcgag ctcggatcga ggctctctcg caggtctccc gagagatgga gctcgattcc
    16501 ctcgggattc gcctcgacgt ccttcacgaa gtacgcaggg ccttcgatct cgacgtaggc
    16561 ccagttgatc gagttgctga gacagaaacg accgtcctcg gcgcgatcga cccagctgtc
    16621 gaacgaacga gcgagcttgg ggtgctcgat ccggtcctcg ccattgaacc agcgcccgct
    16681 ggcgtcgcgg cggatctcgg tctccctggt tcgcccctta agaaactgcg ctgggtccat
    16741 gaccccacgc agactacaat gcccttcgat ggtgagaaag agccgaaagc tcgtggtcgt
    16801 ttcgaaccgc ggcccttatc ggcacgaagc cgcccgaggc aaggaacgct gggtccgctc
    16861 cgccggtggc ctcgtcgctg cgctcgaccc ggttctacag atgcgcggcg gcgtctgggt
    16921 aagcgccaaa ccggccaagg acttcgattc ggtcaccgtt ccatcgcctg acctcgcgta
    16981 cgaccttgct cacatctcac tcaagagggg cgatcagcgc ggattctacg aagggatttc
    17041 gaacgccgtg ctctggccgc tcctgcacgg tttcgagccg acgattcagg tcggtgaagc
    17101 cccctggtcg agttatctcg gggcgaatca ggctttcgca gacaccacct tggccaccag
    17161 cggaccaagc gacctgatat ggattcagga ctatcacctc atgctcgtgc cggcgatgat
    17221 ccgcgccaag cgcagcaagg cgcgaatcgg ctggttctgc cacatcccct ggcctccgcc
    17281 cgacaccttc ggcatcctcc cctggcgcga ggagatcctc gaagggcttc tcggggcgga
    17341 cgtcctcggg ttccatcttc cggagtacgc ggagcatttt cgagcctgcg tcgaacgatt
    17401 cacgacctac cgggtctctc gtggggcgat tcgatatcgg ggacggtcgg taaagaccat
    17461 cgcggcgccg atcgggattc cagtcgatga attgcaggcg ctcgcgatcg acccagacgt
    17521 cggacgcgag gtggagcgca ttcgccaact gatggtcaac cgtaagctca ttctcggcgt
    17581 cgaccgcttg gactacacga aaggcatccc agaacggctc gctgcattcg agcgcttgct
    17641 cgcccgggac aaggacgcac gcaagcggta cgcgctgatt cagatcatgg ttccgagccg
    17701 aaccgacgtt caagcctacg ccgacctcaa acaggagatc gatcggatgg taggcgacat
    17761 caatggccgc tatgcggaga ccggccgcgt gccgattcac tacctctacc gaaatctgag
    17821 ccagcgcgcc ctattcgcgc actatcgtgc agcggacgtg gccctcgtga cgccattgcg
    17881 cgacggcatg aacctggtgg ctcacgagta tgccgcggcg cgaaccgacg aagacggcgt
    17941 gctgatcctc agcgaattcg ctggagccgc aaagcatctc aagggcgcga tgctggtcaa
    18001 cccgtacgac gtcgattcga ccaccaacgc gattcatcgc gcgctcacga tgaagcctgc
    18061 cgagaagcgc aaacgcatgc gttcgctgcg cgaggaggtc atgcgcctgg acgtgcacct
    18121 ctgggccgat cgctacatcg ccgccctcga gggaaaatga cgcgcatcga ttccctgatc
    18181 gagcggcttc tccgactgga gcgccccatg cttttggcgt tcgacgtcga cggaacgctg
    18241 tctccgatcg tccgtgaccc ggacctcgct cgcattcccg aagaaacgct ccgcaccatc
    18301 gcggagctcg tcgaatcacc gatgaccgag atcgcgctga tcaccggtcg agatctgagc
    18361 tcgctcgggc gaatggagca gctggatggg atctggcgcg ccgtcgagca cggaggtctg
    18421 gtcctcgctc cgggtgacag acccgcgcct cgccagctca gggcggagca agaagccgcg
    18481 ctcgatcgat ttcggcactg ggtcgacgcg cacgccgagg gcgctttcat cgagtacaag
    18541 ccccaagcca tcgccgtgca cgttcgcgcg atcgccgaaa cggaccctga gcgcg
//
LOCUS       DASRZF010000335        14001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3356589, whole genome shotgun sequence.
ACCESSION   DASRZF010000335 DASRZF010000000
VERSION     DASRZF010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 14001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14001
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3356589"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VFG22_14795"
     CDS             complement(<1..300)
                     /locus_tag="VFG22_14795"
                     /inference="COORDINATES: protein motif:HMM:NF024940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6417562.1"
                     /translation="MDSGLVVMSRREIERAHVMRAVQERRLTQKQAASQLALSVRQVE
                     RLYARYKRDGPTGLVSRRRGQRNHVLPDGLREEALSIVRTRYSDFGPTLAHEKLLE"
     gene            complement(450..845)
                     /locus_tag="VFG22_14800"
     CDS             complement(450..845)
                     /locus_tag="VFG22_14800"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HET6417563.1"
                     /translation="MASSGARLIVSSLLAAIFVAGAPTDGKASYSGQLDAFAVRQKIQ
                     KDLPKINRCYESALRYEPELSGKVSVRFAVIRNGYVKGVQVLENTTGNDKVERCVARV
                     VSALRFPSRRTGKSLRFTFPFVFAPQAMR"
     gene            952..1986
                     /locus_tag="VFG22_14805"
     CDS             952..1986
                     /locus_tag="VFG22_14805"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6417564.1"
                     /translation="MRRTPSELSCLERTKMRILRHTHSVLGEGLVGSLSRLGRLHPLS
                     NPSRYGVQVIRDVEYLQSGNPRHRLDIYRPSKSVGTVPIVIYMHGGGFNLLSKNTHWL
                     MGLAFARAGYLVFNVDYRLAPQNPFPAAVSDTCAALEWVAKNAEAYGGDLGRLVFAGE
                     SAGGNLATALTICCCYRRPEPFAQRAFATGLVPTAVAPACAPLQVSNPERLNRKRKLP
                     RWVNDILEGMNESYIGTLHAPLPGELDLADPLHVFERREHPDRALPAFFVPIGTRDPL
                     LDDTRRLDRALKAMGVECEARYYEGELHAFHALVWRRQARRCWREQLAFLARALQHPR
                     EPRHEFVAQA"
     gene            complement(1997..3280)
                     /locus_tag="VFG22_14810"
     CDS             complement(1997..3280)
                     /locus_tag="VFG22_14810"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase family
                     protein"
                     /protein_id="HET6417565.1"
                     /translation="MEPLARSLTLPCGAILSNRIAKAAMTEGLADPDDRASEGLATLY
                     GRWSDGGAGLLLTGNVMVDRRYLERPGNVVIDGNGGEAGLRTWAAAGTRSGNHLWMQI
                     SHPGRQCTRMSSSHPVSPSSEKLQGMMGLIAPPRALGVDEIHEIIERFAEVARTAKDN
                     GFTGVQIHSAHGYLSSQFLSPRANKRTDEWGGSLENRARFLLETYAAVRAAVGSDFPI
                     AVKLNSADFQKGGFSKEESARVAGWLAERGLDLLEISGGTYEQMALLGRGDASAEAKA
                     ESTRQREAYFLEYARDIRKSAEGLPLMVTGGFRTPQLMRDVLDSGEVDLIGIARPFCV
                     APDFPRELLAGGLEALPMPEKEIRLGPGIFGRASSSRSMRTLNSQAEVAWFYRQIIEM
                     SEGREPDPRFGTWSCLFGHFAGEFRRARRRTFAEG"
     gene            complement(3318..3869)
                     /locus_tag="VFG22_14815"
     CDS             complement(3318..3869)
                     /locus_tag="VFG22_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417566.1"
                     /translation="MANETEELWQSEVLVSDSIGRLIEFWGFKRNMGRLWAVLYLTDQ
                     ALSAPDIQERLQLSSGAVSMTLNELTRWGVVKKVWLQGERRDHYTAEGNLWKMISRVF
                     NERERVEVLDAIDIMEDAIRFVKDKAKEPQERARAQFQEARIRELLDLARLGKQLIDA
                     LIKDAKVDGSPLMSVLLGEAEKR"
     gene            3934..4218
                     /locus_tag="VFG22_14820"
     CDS             3934..4218
                     /locus_tag="VFG22_14820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417567.1"
                     /translation="MERPTFEAMLEAADGVEQNGNDYSVAEGYSLCVYVGQPGQAMEV
                     SEVGALRLDTAFCEATSREHNSVYFLEYSSLHGLCVRPPAGSGGRRAGFS"
     gene            4283..5668
                     /locus_tag="VFG22_14825"
     CDS             4283..5668
                     /locus_tag="VFG22_14825"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6417568.1"
                     /translation="MSARILVCDDEASLREMLRILLSREGYRVDVVDGVHAAREQLGS
                     ADPYDVVITDLVMPDGTGMEVLDAARARSPDTQTLMITAYATTEQAVEAMRKGAYDYV
                     QKPFRNHELLATLEKALEKRAIVAENQTLREQVKARWTEGQLIGKSAAMDRLRDLIRR
                     VANTTTSVLITGESGTGKEMVARALHFQSPRAEEAFVVLNCGAVPENLIESELFGHVK
                     GAFTGATSAKEGLFRAADHGTLFLDEIGELPPQLQVKLLRVLQDQRVRPVGGSAELEV
                     DVRVVAATNRDVESEVEAGTFREDLFYRLNVIRIEVPPLRERPEDIPVLANYFLQKHA
                     AVQERRLEFSPEAMRWLAQQSFAGNVRELENMVERGVTLSPGSTVNREDLGDTHPSKK
                     ALNLSQIPPGKFDLDEYLAQVERELLFRALDQAGGVRTKAADLLGMSFRQFRYRLAKH
                     ISPELGDVAGD"
     gene            5694..6221
                     /locus_tag="VFG22_14830"
     CDS             5694..6221
                     /locus_tag="VFG22_14830"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET6417569.1"
                     /translation="MSRHPRSAGFTTIELLIGVAIVGILAALAVPTFKGYVYRGRVSE
                     AVTVLNEIKSRQEAYRSRFGNYAAVSGGGDWSSATYTPSTIPGVQAVAWPSSANWEEL
                     GLSSPGTVRFRYATVAGQPGSTPPTGSNLDNRNFWYAAQAEGDLDGDGNTFILEVYSD
                     SRIMYNSAVGQGGWE"
     gene            6218..6727
                     /locus_tag="VFG22_14835"
     CDS             6218..6727
                     /locus_tag="VFG22_14835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET6417570.1"
                     /translation="MRQKQGFTITELMIAVVIMGVLATLAIPTFTGYIYKARVTEASN
                     FLGEIKQRQESYRNEFGQYCAVNGDDWGTYNPSTIPGIDPVGWVTTPEWQQLGAAPDG
                     PIRFQYATVAGVPGVAAPSGTNLDTDDHWFAARAQGDLDEDGTDFFLEIYSQSNHIYN
                     SATAFGGWE"
     gene            6914..7486
                     /locus_tag="VFG22_14840"
     CDS             6914..7486
                     /locus_tag="VFG22_14840"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET6417571.1"
                     /translation="MNKLLSKKEGFTLIELMIVVAIIGILAAIAIPAFINYVKRSKTS
                     EAGANLKSLFQGAAAYYEAENWGQGMVTAGGTAAASTHCTVSSAAQSTTPSDVKQIID
                     WSAETDAASFTALNFAPADPIYFDYNVTADPAATACGNGPSDTGVYTFTANGDLDGDS
                     TLSTFSLAAGTNPDNALYRSPGIYSNNPLE"
     gene            7578..8426
                     /locus_tag="VFG22_14845"
     CDS             7578..8426
                     /locus_tag="VFG22_14845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417572.1"
                     /translation="MRIGVRVVAVVLMLGALAGIWSLRGRSMAHYLGTQTYEDIYYLP
                     PPQWLQVMSLGHRRALADLIWLRALIYFGDEFENRGAVKHVFNYGESMLALDPDFRRV
                     YRWVGVAGVYTPTGSPPEFIERAIDVLRRGVERFPDDGELAWDAGATITYELLPNLAK
                     EDPNRERLSAEGNEYMMAAARLGAGPEWLVITNATSLRKLGERDRELRHLEEMYAIIR
                     DPTVRAQIETRLSQLRDQAYAEAFRNANEEFEQRRREEFPYLPSTLYFFVADPIETTA
                     GLEGAS"
     gene            8499..9002
                     /locus_tag="VFG22_14850"
     CDS             8499..9002
                     /locus_tag="VFG22_14850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417573.1"
                     /translation="MEAALLLCILGSVLAVFVPTFLRRVRTNKISEAAELLQEMSERA
                     AAYYATSWDSGLRHCLPPPAGPTPGAPSVDPAEVDFFVPEQLGHETWSALDFQPDRPI
                     RYSYSYRPDENGCDLEGSKGAGIVSFRAEGDLDGDGVRSTFERRATLEAGVLAPEGVL
                     HVHQRIE"
     gene            complement(9019..9828)
                     /locus_tag="VFG22_14855"
     CDS             complement(9019..9828)
                     /locus_tag="VFG22_14855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417574.1"
                     /translation="MRARSSATTVCRVLIGLDRAEASGTLQVLAGGRSALIFLETGQV
                     TGANVDRRVATSQRQVFERFLEICNWDDVVLRLVGLSEAASWWKLQEPIPARTLALQT
                     MRAAVNGSELSSMRADLGDAVYHLTDSGEALSRLAVLRPEETAVLFWLRRGVRAEELA
                     GLPGCGMTGYRFLWMLKLLGAAAPRTNGSYPLLLRKRRELRNDASAHALLDLPEGAGG
                     RDARLALRKLVRDIHPDRFGDGAPLALRRASGEIVSALVDAEARIASRNRD"
     gene            complement(9869..10804)
                     /locus_tag="VFG22_14860"
     CDS             complement(9869..10804)
                     /locus_tag="VFG22_14860"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6417575.1"
                     /translation="MGERAITVEELWEQAEEALEASSKRAEALLTELARAAEEGSDAS
                     LFAHRHLAELHLEENPWKAALHLRRVLTFCPDDDMLHALMGLSHALLGNYRVAVRAYC
                     EASSLAPQTPWYHHNLGHLMDVALDDPSAAEHHLRRAYQLEPEHDEIIGSLAHCVARL
                     GDLDEAQRLAELAHEKGPFNPDHRALLKWIEDGATDAPATAATVRRPPGDPVAETVEL
                     KMGEAGFSREEIDRATQLWSDMKAHCELRVTKPEVLAAALEYAIAVVERRPGCTQAEV
                     ARRYGVAPTSVSARFAQIRNALALRPGDPRYAASR"
     gene            11027..11500
                     /locus_tag="VFG22_14865"
     CDS             11027..11500
                     /locus_tag="VFG22_14865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417576.1"
                     /translation="MPEEYDEKASADPERERRWSEGVLRDTLRKAVERGIEAGVGTLR
                     SADSAIRGVTQDGRLPKEVIGYVFASVDETKNALVRGVAKEVRDFLEATDIAGEFYRA
                     LTSLSFEVKTEIRFIPNDAGGVRPQVKARAVQKRSRRKGVVETEPIIPPDPSGEP"
     gene            11506..13047
                     /gene="cobA"
                     /locus_tag="VFG22_14870"
     CDS             11506..13047
                     /gene="cobA"
                     /locus_tag="VFG22_14870"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HET6417577.1"
                     /translation="MRSAGKVYLVGAGPGDPELITARGLRRLREADIVLYDALVHPDQ
                     LNAARQDAELIFVGKRAGRASQRQAEINRQLAQAARDGKIVVRLKGGDPYLFGRGSEE
                     AELLAAEGIPFEVVPGVPSPLAATAYAGLSLTHRELASSVAYITATESVEKDRTGHDW
                     SKLATATQTLVIFMGMRKLDSLMKLLIEHGRPADTPVAVVQWASLPKQRTVVGTLADI
                     HERAAEAGLGLPALTIVGEVVRLRRNLRWFDSKPLFGKRILVTRAVEQAGSLATLLRD
                     EGAQPLLAPTIRIAPPLDPGPLRDAVTHLDCYDWILFTSTNSVQALFTFLSDASLDLR
                     ALAKAKVCAIGVKTRDALLAQGLRPDLVPEDARAEGVIAALRPHLRPRTRILLPRAEV
                     AREILPDSLRDAGAEVDVVTAYRNLPPEPREVGRIRSLVDPGECDCVLFTSSSTVQNL
                     VDLLDEGARKRLNSLDLFSIGPITTQTAERLGLRIAATSAEQTIEALVETLRAYYAPE
                     QDADA"
     gene            13040..13441
                     /locus_tag="VFG22_14875"
     CDS             13040..13441
                     /locus_tag="VFG22_14875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417578.1"
                     /translation="MHSVTKLAGLAALGLLFTACGDPCGDPEDRLVQLSELPCNMTNA
                     DGVWQSHPWPPVADEECYWLEFRGCSQYEIEHPLGRIPALVVGYTSFDPDGGFATIGS
                     GNSFIVEEASTSTVTIRNDQNQLFYLKLVLE"
     gene            13536..13868
                     /locus_tag="VFG22_14880"
     CDS             13536..13868
                     /locus_tag="VFG22_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HET6417579.1"
                     /translation="MGERFENDAGTITQVKPEKKLKRPRLYRVLLHNDDYTTREFVVQ
                     ILHTVFHLGEQAAIRVMLHVHNNGVGVAGVFTREVAETKIKRVEQLAREQEFPLRLTM
                     EPEEEEDA"
BASE COUNT         2688 a         4496 c         4292 g         2525 t
ORIGIN      
        1 ctcgaggagc ttctcgtgcg ccaacgtcgg gccgaaatcc gaataccgcg tccggacgat
       61 gctcagggcc tcctcacgca gcccgtcggg caagacgtga ttgcgctggc cacgccgacg
      121 cgagacgagt ccagtgggtc cgtcacgctt gtagcgagcg tagagccgct ccacctgacg
      181 cacgctcagc gcgagctgtg acgcggcctg tttctgggta agccggcgct cttggaccgc
      241 acgcatcacg tgcgctctct cgatctcgcg ccggctcatg acgacgagcc cggagtccat
      301 tgggggcggg taaacccgac atttgaatct ggcacaaacc cgacacttct atattgtttc
      361 tacacatcaa tttcgcagaa cgtatattat gtaaactttt ctgacactgg cgctgacact
      421 ggcgctgaca ctgccgctga cactgctggc tagcgcatgg cctgcggcgc gaagacgaat
      481 gggaacgtga atcgcaggct cttgccagtc cggcgactgg ggaatcgaag cgcgctcacc
      541 acccgcgcca cgcagcgttc gaccttgtcg ttccccgtgg tgttctcgag aacctgcacg
      601 cctttgacgt aaccattgcg tattacggcg aaacgaaccg agaccttccc ggagagctcg
      661 ggctcgtaac gaagcgccga ctcgtagcag cgattgatct tcggaaggtc tttctggatc
      721 ttctgccgaa cggcgaaggc atcgagctgg cccgaatagc tggccttgcc gtccgtcgga
      781 gctccggcga cgaaaatcgc cgcaagaagc gacgagacga tcaaccttgc acccgaggaa
      841 gccatccccg agggatacgc cgtccttggg ttttcccgca aaaaatcggc atttcctagc
      901 ccccgatcgc caaatccgtc gagatcggtg ggagcgtgat acaagttgca aatgcgccga
      961 acgccatcgg aactgtcctg tctagagcgc accaagatgc ggatccttcg ccacacccat
     1021 tcggttctcg gcgaaggcct ggtcggcagc ctctcacgac tcggacgtct gcacccgctc
     1081 tcgaacccgt cacggtatgg tgtacaagtg atcagggacg tggagtatct ccaaagtggc
     1141 aatccacggc accgactcga catctatcgg ccgagcaagt ccgtcggcac cgttccgatc
     1201 gtcatctaca tgcacggcgg cggcttcaac ctcttgtcga agaacaccca ctggctcatg
     1261 ggcttggcgt tcgcgcgcgc cggctatctg gtgttcaacg tcgattatcg actcgcaccc
     1321 caaaaccctt tcccggccgc ggtctctgac acctgcgcgg cgctcgaatg ggttgccaaa
     1381 aacgccgaag cgtatggcgg cgacttgggc cgactggtgt tcgctggtga gtcggccggt
     1441 ggtaatctcg cgaccgcgct caccatttgt tgctgctacc ggcgccccga accctttgcg
     1501 caacgcgcgt tcgccacagg gctcgtgccg actgctgtgg cgccagcctg cgcaccgctc
     1561 caggtcagca accccgaacg cttgaaccgc aaacggaagc tcccgcgctg ggtcaacgac
     1621 attctagaag gcatgaacga gtcgtacatc ggaacgcttc acgcccctct ccccggtgag
     1681 ctcgatctcg ccgacccgct acacgtcttc gaacggcgag agcatccgga ccgcgccctt
     1741 cccgcgttct tcgtgcccat cggtacgcgc gatcctctgc tcgacgatac gcgccggctc
     1801 gaccgcgcgc tcaaagcgat gggcgtagaa tgcgaagcgc ggtactacga aggcgagtta
     1861 cacgcgttcc atgcgctcgt ttggagacgc caggcgcgac gatgctggcg agaacagctc
     1921 gcgttcctgg cacgcgcgct tcagcatccg cgcgagccgc gccacgagtt cgtcgcgcaa
     1981 gcctaggagc tcggagctat ccttcagcga acgttcgccg ccgcgcccgg cgaaactcac
     2041 ccgcgaagtg gccaaacaag cacgaccagg ttccgaatct gggatcaggt tcgcgcccct
     2101 cgctcatctc gatgatctgt cgatagaacc aggcgacctc ggcttggctg ttcagagttc
     2161 gcatcgagcg gctgctgctc gcgcgaccga agataccggg gcccaggcga atctccttct
     2221 cgggcatggg tagtgcttcg agaccgcctg cgagcagctc gcgggggaag tccggggcga
     2281 cgcagaacgg tcgagcgatc ccgatcaaat cgacctctcc cgagtcgagg acatcacgca
     2341 tcaactgcgg cgttcgaaat ccgcccgtca ccatgagcgg taggccttcg gccgacttcc
     2401 gtatgtcgcg agcgtattcg aggaagtacg cctctcgttg ccgtgtgctc tccgctttgg
     2461 cttcggcaga agcgtcgccg cgcccgagca gcgccatttg ctcgtaggtg ccgcccgaga
     2521 tctcgaggag atcgagcccc cgctcggcaa gccagcctgc gacacgtgcc gactcctctt
     2581 tgctgaagcc gcccttctgg aaatcggcgg agttgagctt gaccgcgatc gggaaatcgc
     2641 tcccgacggc tgcacggacg gcggcgtagg tctcgagcag aaagcgcgcc ctgttctcca
     2701 aagaaccgcc ccactcgtcc gttctcttgt tggcacgcgg cgacaagaac tgactcgaga
     2761 gatagccgtg cgcggagtgg atctgcacgc cggtgaagcc gttgtccttg gcagttcttg
     2821 caacctcggc gaagcgttcg atgatctcgt gaatctcgtc gacgccaagt gcgcgaggag
     2881 gcgcgatcaa gcccatcatt ccctggagct tctccgatga aggagagacc ggatgactgc
     2941 tcgacatgcg cgtgcactgc cgccccggat gactgatctg catccagaga tggttcccgg
     3001 agcgcgttcc agcggccgcc caggttcgga gcccggcctc ccctccgttc ccgtcgatca
     3061 cgacgttccc gggtcgctcc aggtatcgac ggtcgaccat cacgttgccg gtcaagagca
     3121 gcccggcacc accatcggac cagcgtccgt acagagtggc cagaccctcc gaggctcggt
     3181 cgtccgggtc agccagacct tcggtcatcg ccgccttggc gattctgtta cttaggatcg
     3241 cgccgcaagg cagcgtcaag cttcgagcga ggggttccat gaggagcgga gtctagacgg
     3301 atttggaccg gatgcgacta gcgcttttcg gcttcaccga gaagtacgct catcagcggg
     3361 cttccgtcca ccttcgcatc cttgatcagg gcatcgatca actgcttgcc gaggcgcgcg
     3421 agatcgagaa gctcgcgaat acgagcctct tggaactgcg ccctcgcccg ctcctgcggt
     3481 tccttggcct tgtccttcac gaaccgaatg gcgtcttcca tgatgtcgat cgcgtcgagg
     3541 acctcgaccc gttctcgctc gttgaagacc cgggagatca tcttccagag gttgccctcg
     3601 gcggtatagt ggtctcgccg ttcgccctga agccagacct tcttgacgac gccccagcgc
     3661 gtcagctcgt tcagagtcat cgaaaccgca ccgctgctga gttgcagtcg ctcttggata
     3721 tctggtgcgc tgagggcctg atcggtgaga tagaggaccg cccagaggcg ccccatgttt
     3781 cgcttgaacc cccagaactc aatcagtcgg ccgatcgaat cgctgaccag gacctcgctt
     3841 tgccagagct cctccgtttc gttcgccact gtagcggcct accctgaaca ccatggcttg
     3901 accagccgcc aagtcccatc aaagctgcgg cgcatggaac gacctacgtt cgaggccatg
     3961 ctcgaggcag cagacggtgt ggagcaaaac ggaaacgact actccgtcgc cgagggctac
     4021 agcttgtgtg tctatgtcgg ccagccgggc caggcgatgg aagtgtccga ggttggtgcg
     4081 ctgaggctcg acaccgcatt ttgcgaggcc acatcgcgtg agcacaattc cgtgtacttc
     4141 cttgagtaca gcagcttaca cgggctttgc gttcggccgc cggcgggcag cggtggtcgt
     4201 cgcgctggtt tcagctgagg cgtgcaagtc tcgtggccgc aggccggttt tgacgtagga
     4261 tgtgaacggg ggtgcatcga acgtgtccgc acgcattctt gtctgtgacg acgaagccag
     4321 cttgcgcgaa atgctgcgga tcttgctgag ccgtgagggc taccgtgtcg acgtggtcga
     4381 tggcgttcac gcggcgcgcg aacagctcgg aagcgccgat ccctacgacg tcgtcatcac
     4441 agacctcgtg atgccggatg gaacgggcat ggaggtgctc gacgccgcac gagctcgctc
     4501 cccagacacg caaacactca tgatcaccgc gtacgccacg accgaacagg cggtcgaggc
     4561 catgcgaaaa ggcgcttacg actacgtcca gaagcccttc agaaaccacg agctgcttgc
     4621 cacgctcgaa aaggcgctcg agaagcgcgc gatcgtcgcg gagaaccaga cgcttcgcga
     4681 gcaggtcaaa gcgcgatgga ccgaaggcca gctcattgga aagagcgcgg cgatggaccg
     4741 gctccgcgac ctgatccgac gtgtcgcgaa cacaacgacg agcgtgctca tcaccggcga
     4801 gagcggcaca ggcaaggaga tggtggcacg cgcgctccac ttccagagcc cgcgcgccga
     4861 agaggcgttc gtcgtgttga actgcggcgc tgtccccgag aatctgatcg agagcgagct
     4921 cttcggtcac gtcaaaggtg cgttcaccgg agccacctcg gcgaaggagg gcctcttccg
     4981 tgctgccgat catggcacct tgttcttgga cgagatcggg gagctgccgc cgcagttgca
     5041 ggtgaagctt ttgagagtcc ttcaggacca gcgagtgcga cctgtgggcg gcagcgctga
     5101 gctcgaggtc gacgtccggg tggtcgctgc gaccaaccgg gatgtcgagt ccgaggtgga
     5161 ggccggaaca tttcgcgagg acctcttcta tcgactgaac gtgatccgca tcgaggtccc
     5221 gcccttgcgc gagcgacccg aggacattcc ggtgctggcc aactactttc ttcagaagca
     5281 tgcggccgtg caggagcgtc gcctggagtt ttcgcccgaa gcgatgcgct ggttggcgca
     5341 gcagtcgttc gccgggaatg tccgtgagct cgagaatatg gtcgagcgag gcgtgacgct
     5401 ctcgcctggc agcaccgtca accgcgagga cctcggggac acgcatcctt ccaagaaggc
     5461 gctgaatctc tcccagatcc cacccgggaa attcgacttg gacgaatacc tcgctcaggt
     5521 cgagagggag ctgctctttc gcgcgctcga tcaggccggc ggcgtccgaa cgaaggctgc
     5581 agatctgctc gggatgagct ttcgtcaatt ccgatatcgt ttggcaaaac acatatcgcc
     5641 cgaactgggc gatgtggccg gcgattgata tagtgggcct tggaggctct tctttgtcgc
     5701 gacatccccg aagcgctggc ttcacaacca tcgaattgtt aatcggcgtc gcgatcgtcg
     5761 gcatcttggc ggcgctcgcc gttccgacgt tcaagggcta cgtgtaccgc ggccgcgtca
     5821 gtgaagcggt cacggtcctc aacgagatca agtcgcgtca ggaagcgtat cggagccgtt
     5881 tcggcaacta cgcggcggtc agcgggggcg gcgactggtc gtctgcgacc tacacgccgt
     5941 cgaccattcc cggggttcaa gccgtagcct ggcccagctc cgcgaactgg gaggagctcg
     6001 gcctcagctc gccgggaacg gtgcgatttc gctacgccac ggtggccggg caacccggat
     6061 cgacgccccc aaccgggagc aatctcgaca ataggaattt ctggtacgcc gcacaggccg
     6121 aaggcgacct cgacggggat ggcaacacgt tcatcctcga ggtctattcg gactcgcgca
     6181 tcatgtacaa cagcgcggtc ggccaagggg gatgggaatg aggcagaagc aaggctttac
     6241 cataacggag ctcatgatcg cggtcgtgat catgggcgtc ctggcgaccc tcgcgattcc
     6301 gaccttcacc ggctacatct acaaggcccg tgtgaccgag gcgtcgaact tcctcggcga
     6361 gatcaagcag cgtcaggaat cctaccggaa cgaatttggg cagtactgcg cggtcaacgg
     6421 cgacgactgg ggcacctaca acccttccac catcccgggc atcgacccgg ttggctgggt
     6481 gaccaccccc gaatggcagc agctaggagc agccccggac gggcccattc gctttcagta
     6541 cgccaccgtg gccggcgtcc ccggcgtggc cgctccgagc gggaccaacc tagacacgga
     6601 tgaccattgg ttcgccgcac gggctcaggg ggatctcgac gaggacggca cggatttctt
     6661 cctcgaaatc tactctcaat cgaaccacat ctataacagt gccactgcat ttggtggatg
     6721 ggagtgacaa ttcccgtcac cttcgggtga cgagaactgt ccctcacgcc ccgaccttgg
     6781 cagcgttgat gccggttttg ccaataattt ccgtattggc accgggttcg agagaatcgg
     6841 cacggcagtt gcattgtcct ggtgcaactc tgatttatgc gggcgctggg cccgggggaa
     6901 agggaactaa cgaatgaaca agctactttc gaagaaagag ggatttaccc tcatcgagct
     6961 gatgatcgtt gtcgccatca tcggcattct cgccgcaatc gcgattccgg cgttcatcaa
     7021 ctacgtcaag cgttcgaaga cctcggaagc cggtgcgaac ctcaagtcgc tcttccaggg
     7081 cgcagccgcg tactacgagg ctgagaactg gggccagggc atggtcaccg cgggcggcac
     7141 cgccgcggct tcgacgcact gcaccgtcag ctcggccgct cagagcacca ccccgagcga
     7201 cgtcaagcag attatcgact ggtcggctga gaccgatgcc gcgtcgttca ccgcgctcaa
     7261 cttcgctccg gcggacccga tctacttcga ctacaacgtg accgcggacc cagccgcaac
     7321 ggcttgcggc aacggcccca gcgacaccgg cgtatacaca ttcacggcga acggcgacct
     7381 cgacggcgat tccacgctct cgacgttcag ccttgccgcc ggcaccaacc cggacaatgc
     7441 gctctaccgc tcgccgggca tctacagcaa caacccgctc gagtgatcga cttagggtaa
     7501 tccgtgaaag gccgcggcgg atgtcgcggc cttttgcatt taaggaggtc ggaccccttt
     7561 ttcgctatac tgcaaatgtg cgcattgggg tacgcgtcgt cgccgtcgtt ctgatgctgg
     7621 gagcgctggc agggatttgg tcgcttcgcg ggcgctcgat ggcgcactac ctcggcacgc
     7681 agacctacga ggacatctac tacctgccgc ccccacaatg gctgcaggtg atgagcctcg
     7741 gtcaccggcg cgccctcgcg gacctgatct ggttgcgtgc cctgatctac ttcggcgacg
     7801 aattcgagaa ccggggcgcc gtgaaacacg tcttcaacta tggcgagtcc atgctggccc
     7861 tcgatcccga cttccgtcgc gtataccgct gggtcggagt ggcgggggtc tatacgccga
     7921 caggaagccc acccgagttc atcgagcgag cgatcgacgt cctccggcgc ggagtcgagc
     7981 gctttcccga tgacggggag ctcgcctggg atgccggagc gacaatcacc tacgagctgc
     8041 tcccgaacct cgccaaggaa gacccgaacc gggaacggct gtcggccgaa gggaacgaat
     8101 acatgatggc agcggcgcgc ctgggcgccg gtcccgagtg gctggtcatc accaacgcaa
     8161 cctcgctccg aaagctcggt gaacgggatc gtgagctgcg ccatctcgag gagatgtacg
     8221 cgatcatccg ggatccgacc gtcagagcac agatcgaaac ccggctctcg cagctccgcg
     8281 atcaggccta cgccgaggcc ttccgaaacg cgaacgagga gttcgaacag cgacggcggg
     8341 aagagttccc gtacttgccc tcgacgctct actttttcgt agccgatccg atcgaaacga
     8401 ccgcgggcct cgaaggcgcg agctaggctc ttgccaaagg ccgtcaggcc tcgcagagtc
     8461 ccgcgagctt gtcccgtgcc cgaccgacat tgagcttgat ggaagccgcg ctcttgctct
     8521 gcatcctcgg cagcgttctg gctgtgttcg ttcctacttt cctccggcgg gtccgcacga
     8581 acaagatcag cgaagccgcc gagctcctcc aggagatgag cgagcgtgct gccgcgtact
     8641 acgcaacgtc atgggactcg ggactccgcc actgtcttcc gccccctgcg ggcccaacgc
     8701 ccggggcgcc cagtgtggac cccgcggagg tcgacttctt cgtccccgag cagctaggtc
     8761 acgaaacctg gagtgccctc gattttcagc ccgatcgacc gattcgctac agctacagct
     8821 acaggcctga tgagaacggc tgcgatctag agggcagcaa gggggctggg atcgtctcgt
     8881 ttcgcgccga aggtgatctc gatggagacg gcgttcgctc gacattcgaa cggcgggcga
     8941 cactggaggc gggagtgctc gcgccggagg gcgtgctgca cgtgcatcaa cggatcgagt
     9001 gagcggctcg gagcggtgct agtcgcgatt ccgagaagcg attcgcgcct cggcatcgac
     9061 cagggcgctg acgatctctc cggacgcccg ccgaagggcg agcggcgcac cgtcaccgaa
     9121 gcgatcgggg tggatgtctc tcacgagctt acgaagagca agtcgggcgt cgcgcccccc
     9181 cgccccctcg ggaagatcga gaagcgcatg cgcggacgcg tcgtttcgga gctctctgcg
     9241 cttcctcaag agcaagggat aggaaccgtt cgttcgcgga gcagccgcgc cgagaagctt
     9301 gagcatccac aggaatcggt aaccggtcat gccacagccg ggcagcccag cgagctcctc
     9361 ggcacgaacc cctcggcgca gccagaacaa gaccgcggtc tcctcgggtc gaaggactgc
     9421 gagccgagag agagcctcac cggagtcggt gaggtggtag acggcgtcgc cgagatccgc
     9481 tcgcatgctc gacagctccg aaccattgac ggccgcccgc atggtctgaa gcgccagcgt
     9541 ccgggcggga atcggttctt gcagcttcca ccagctcgcc gcctcgctga gcccgaccag
     9601 gcggagaacg acgtcgtccc agttgcagat ctccaagaag cgctcgaaca cctgacgctg
     9661 cgatgtcgcg acccggcgat cgacgttcgc gcctgtcacc tgtcccgtct cgaggaagat
     9721 gagggcgctg cgtccaccgg caagcacctg caaggttccg ctcgcctccg cccgatcgag
     9781 accgatcagg acgcggcaca ccgtcgtagc actcgaacgt gcgcgcatga atctccggat
     9841 aggcgattca tcgatcgctg gccagttttc agcgtgacgc cgcgtagcgc ggatcgcccg
     9901 gtcgcaaagc cagcgcgttt cgaatctgag cgaagcgtgc cgacaccgac gtgggcgcca
     9961 caccgtatcg tcgcgcgacc tcggcttgcg tgcacccggg acgccgctcg acgaccgcaa
    10021 tcgcgtattc gagggcggca gcgaggacct cgggtttcgt cactcgaagc tcacagtgag
    10081 ctttcatgtc cgaccatagc tgcgtggcgc ggtcgatctc ttcacgcgag aacccggcct
    10141 cgcccatctt caactcgacg gtttccgcaa ccggatcgcc cggcgggcgc cgcaccgttg
    10201 ccgcggtcgc cggggcgtcg gtagcgccgt cttcgatcca cttcagaagc gcgcgatgat
    10261 cggggttgaa ggggcccttt tcgtgggcga gctcggcgag cctttgcgct tcgtcgagat
    10321 cgccgagacg ggcgacgcaa tgggcgagcg aaccgatgat ctcgtcgtgt tccggctcga
    10381 gctggtacgc acgtcgcaag tggtgctcgg ccgcactcgg atcgtcgagc gcaacgtcca
    10441 tgagatgacc gaggttgtga tgataccagg gtgtctgcgg cgccaagctc gacgcctcgc
    10501 aatacgcccg aacggcaaca cgatagtttc caagcagcgc atggctgagg cccatgagcg
    10561 cgtggagcat gtcgtcgtcg ggacaaaaag tgagaacacg ccgcaggtgc aaggcggcct
    10621 tccagggatt ctcctcgaga tgaagctccg cgaggtgccg atgtgcgaag agcgaggcgt
    10681 cgctcccctc ctctgcggcg cgggcgagct ccgtcagcaa agcctcggcg cgcttggaag
    10741 atgcctcgag tgcttcttct gcttgctccc agagctcttc gaccgtgatt gcccgttcac
    10801 ccatcagcat tcgtatagcc gttcactttt ttgaacgcaa gactcgtcga gagtgcgtgg
    10861 caccaccgac gcgtgctccg acacgtctac agagaaggta tcacgcgtgt tgtatgtgaa
    10921 accatccggt ccaccggggg tgagtttcac gaaactgcaa tccgtgcggg catgaggtat
    10981 gcttgcgggg cgaaccctcg agaggttcgc ggaactcgag acgacaatgc cagaagaata
    11041 cgacgaaaaa gcttctgcag acccggaacg agagcgtcga tggtccgaag gcgttcttcg
    11101 cgacacgctt cgcaaagcgg tcgagagagg catcgaagcc ggcgtcggca cacttcggag
    11161 cgccgacagc gccatacgcg gcgtcaccca agatggaagg ctccccaaag aagtgatcgg
    11221 ttacgttttc gcctcggtgg atgagaccaa gaacgccctc gttcggggag tggccaaaga
    11281 agttcgagat ttcctcgaag caaccgacat cgctggggag ttctaccggg cgctcacttc
    11341 gttgtcgttc gaggtcaaaa cggagatccg tttcattccc aacgatgccg ggggcgttcg
    11401 accgcaagtc aaagcccgag cggtgcagaa gcgcagtcgg cgcaagggag tggtcgagac
    11461 cgaaccgatc atcccacccg acccctcggg cgagccctga gcgggatgcg gtctgccgga
    11521 aaagtgtacc tggtgggtgc cggcccgggc gatccagagc tcatcactgc ccgcggactg
    11581 cgacgcttaa gagaagcgga catcgtgctc tacgatgctc tcgtgcaccc cgaccagctc
    11641 aacgcggcga ggcaagatgc agagctgatc ttcgtcggca agcgtgctgg ccgagcgagc
    11701 caacggcagg ccgagatcaa ccgtcaactc gcgcaggccg cccgcgacgg gaaaatcgtc
    11761 gtccgcctca agggcggtga tccgtatcta tttggtcgag gatccgaaga agccgagctc
    11821 cttgccgccg agggcattcc gttcgaagtg gtcccgggtg tcccttcccc gctggccgcg
    11881 accgcctacg cgggcctctc actcacccac cgcgagcttg cttctagcgt cgcttacata
    11941 accgcgaccg aatctgtcga gaaggaccgc accggccacg actggtcgaa gctcgcgacc
    12001 gcgacgcaga cgctcgtcat tttcatgggg atgcgcaagc tcgattcgct gatgaagctc
    12061 ctgatcgagc atgggcgccc tgcggacacg ccagtcgccg tcgttcagtg ggcctccttg
    12121 ccgaagcagc gtacggtcgt cggcacactt gccgacatcc acgaacgcgc agcggaggcg
    12181 ggactgggcc tacccgcgct cacaatcgtc ggagaagtcg ttcgtctacg acgcaacctc
    12241 cgttggttcg acagcaaacc gctcttcggg aagcgcattc tcgtcacgcg ggccgtcgag
    12301 caagcaggct cgctcgcgac gctccttcga gacgaagggg cccaaccgct cctggcgccc
    12361 accatccgga tcgcgcctcc cctagaccct ggccctctgc gggacgctgt gacccatctg
    12421 gactgctacg actggattct gttcacgagc accaacagcg tgcaggccct gttcacgttt
    12481 ctctccgatg cgagcctcga cctacgggcg ctcgcaaagg ccaaggtctg cgcgattggc
    12541 gtcaagacga gggatgccct gcttgcgcaa gggcttcgtc ccgacctcgt tccggaagat
    12601 gcccgggccg aaggagtgat cgcagccctt cgtccgcatt tgcgaccccg cacgcgaatt
    12661 cttctcccac gagcggaagt cgcccgcgag atcttgcccg attcgcttcg cgacgcaggg
    12721 gccgaagtcg acgtcgtgac ggcataccga aaccttccgc ccgagccgcg ggaagtcggt
    12781 cgcatccgaa gcctggtcga tcccggcgaa tgcgactgcg tcttgttcac ttcctcgtcg
    12841 actgttcaaa atctcgtcga tctcttggat gagggggccc gcaaacgact gaactcgctc
    12901 gacctcttct cgatcggccc gattacgacc cagaccgcgg aacgcctcgg ccttcggatc
    12961 gcggccacat ccgccgagca gacgatcgaa gccctggtcg agaccctgcg cgcgtactat
    13021 gcccccgagc aagatgcgga tgcatagcgt tacgaagctg gcgggcctcg ccgcgcttgg
    13081 actcctgttc acggcatgcg gcgacccctg cggcgacccc gaggaccgct tggtgcagct
    13141 cagcgagctc ccttgcaaca tgaccaacgc cgatggggtc tggcagtccc atccctggcc
    13201 gccggtcgcc gacgaggagt gctactggct cgagtttaga gggtgcagcc agtacgagat
    13261 cgagcatccg ctcggccgaa ttccggccct ggtcgtcggc tacacctcat tcgaccctga
    13321 cggcggcttc gcgacgatcg gatcgggcaa cagcttcatc gtcgaggaag cgagcacatc
    13381 gaccgtgacg attcgcaacg atcagaacca gctcttctac ctgaaactcg tgctcgaatg
    13441 acccccctag cgcatgtcgg acaccggcct tagattctca ggaatgccag atgaaaccgg
    13501 gctgggccgg gtcacagaaa gcgctaaaat agggtatggg cgaaaggttc gagaacgacg
    13561 ccgggacgat cactcaggtc aaaccggaga aaaagctcaa acgaccgcgg ctctaccggg
    13621 ttctcttgca caatgatgac tacacaacca gggagttcgt ggttcagatt ttacatacgg
    13681 tattccacct cggagagcag gctgcgatcc gagtcatgct gcacgtccat aacaatggcg
    13741 tcggcgtcgc gggcgtgttc acccgcgagg ttgcagaaac caagatcaag agagtcgaac
    13801 aactggcccg cgagcaggag ttcccactcc gcttgacgat ggagcccgaa gaggaggaag
    13861 acgcgtaaac atggctggcc ccgtcatcga caaagagctt caagagacgc ttcgcaaggc
    13921 attcgaccgt gcaggcttga tgcgccacga gtacgtgacg ctcgagcacc tgctcctagc
    13981 cctgacggag gatccgaatg c
//
LOCUS       DASRZF010000336        19961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1287790, whole genome shotgun sequence.
ACCESSION   DASRZF010000336 DASRZF010000000
VERSION     DASRZF010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 19961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19961
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1287790"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VFG22_14885"
     CDS             complement(<1..271)
                     /locus_tag="VFG22_14885"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET6417580.1"
                     /translation="MPRVEQEFARAFASDWIDAWNRRDLDAVLVHYTDDFELSSPVII
                     DAVGEPSGTLRGKPAIREYWTKALARLPDLHFDLDEVLTGIDTITL"
     gene            complement(283..1452)
                     /locus_tag="VFG22_14890"
     CDS             complement(283..1452)
                     /locus_tag="VFG22_14890"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HET6417581.1"
                     /translation="MGKIVVVDAVRSAVGRAKKGSLANRRPDELAGEVIRALMARNPG
                     VDPATVEDLVLGCAFPEGEQGMNVARMVGRLGGLPEESSGMTINRFCSSGLQAIALAA
                     GSVKAGFDDVVIAGGMESMTMVPMTGNKPSASPEVMEKYPETYTPMGITAENVANRFK
                     ISRTQQDEFAAWSHEKALAAIKRGAFVDEIVPVHGVTFDKNGERQMFEFKVDEMPRPG
                     TTVEALSQLRPVFAAKGSVTAGNSSPLSDGAAAAIVMKKKRAEKLGIAPMAIFKSFVT
                     VGVDPSIMGIGPLPAVQKLLRKNDLSIDDIDIFEVNEAFASQAVYVQKELGIPNDKLN
                     VNGGAIALGHPLGCTGAKLTATALNELKRRGGKYAVVTMCIGGGMGAAGLFEAID"
     gene            complement(1497..3863)
                     /locus_tag="VFG22_14895"
     CDS             complement(1497..3863)
                     /locus_tag="VFG22_14895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HET6417582.1"
                     /translation="MTKPVRRAGVIGAGVMGSGIMAHFANAGVDVVMLDIVPPGLSDE
                     EKKNPAKRNQFAAGGLQGALKAKPAAFFHPNYAKRVTVGNLEDDIGLLKGCDLVIEAI
                     IENIDIKRSLFEKLKKTLDEGTVVASNTSGLRIADMLHGRGEAFRKNFLVMHFFNPVR
                     YMKLLELVAGEETDPATVERMKVFGENQLGKGIVIGKDTPNFVGNRIGCYSMSLTMNE
                     MIDAGLTPEDVDAITGPPMGHPKSASFRTADMVGLDTFKHVSDNCYEALVDDPERDVF
                     KPPHFLVAMVEQKILGNKTRGGFYKKTREGIETFDPVKLEYRPKAGDEDIKKFCKGLK
                     GSPADRVKALVANDGPAGKFAWNVLSRTLAYSATMIGEIADEVEAVDNAMKWGYNWDL
                     GPFEAWDAIGFKAAYERMKADGLSIPASIDRMAESGAQSFYTDDGRVFSLVTGEYEAR
                     EIDPRNASLTIMRQGDAPVFSNRGSEAWDLGDGILGLTFTTKANSIDDTVIEGITKAV
                     EIAESDFRGMVIYNDGDHFCVGANLFAVVMAAQQKAWDQLRATISGLQGGLQRMKYST
                     IPVVAAPFGMTVGGGFEVCMGADAVQAASETYVGLVEVGVGLLPGGAGNMNMLWRALE
                     GIPDGTDVDTLPFVSRTFQNIAMARVATGAGEAREFGYFRKSDGISFDKARLLTEAKG
                     RAIGMAEAGYHPPVRRAYRLPGESGIATLEMMIDSLEAGGFASAHDALIARKVASVLC
                     GGPSGNAHEVTEEQMLELEREAFVSLCGEPKSQERMQHMLTTNKPLRN"
     gene            complement(3950..4753)
                     /locus_tag="VFG22_14900"
     CDS             complement(3950..4753)
                     /locus_tag="VFG22_14900"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET6417583.1"
                     /translation="MSHGFDIRRIGGPGGLRALEDVTRRAGDAALKHFRQGVVPEKKP
                     DRSPVTIADREAEEIIRDHVLAAHPDAGFLGEETGTHKGSTGIRFIVDPIDGTRAFVR
                     GWPTWSVLMGVEADGVPVVGIAYMPADGDFFVAVQGGGATHNGNTVRVSKVSPLADAT
                     VTHGGLQQFTIAGVGEALLRLSDACDIARGCPDFDGYRQILLGRSDAMVDPGVQPYDI
                     CAPAVLIREAGGRFTSFRGEETIYGGGAIASNGLIHDELVAALTATPYK"
     gene            4859..5788
                     /locus_tag="VFG22_14905"
     CDS             4859..5788
                     /locus_tag="VFG22_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET6417584.1"
                     /translation="MAQGGSIVPFAGWRRSRLRGESPLRQAVRSVGDRVVRFAEEVVG
                     QDLDARIAGISTHLNEVGHDPFGFDPETSRYALALAALLHRRYFRTEVFGIERVPQGR
                     VLIVANHSGQLPVDAVMIASALMLDADPPRFPRSMVETWTAELPFISTLYPRLGQVIG
                     SPENARRLLRQEETLIVFPEGARGISKTFDQRYQLTDFGLGFIRLALETGTPIVPVAV
                     IGGEEQYPSIADFKPLARLLKMPAFPVMPQVFFGMIAPLPTKYRLYFGEPFLFGGDPD
                     DDDRAIEEKVWLVKSTIQSMVNRGLRERKGVFI"
     gene            5803..6927
                     /locus_tag="VFG22_14910"
     CDS             5803..6927
                     /locus_tag="VFG22_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET6417585.1"
                     /translation="MPNPQATNRNKPSARRGRNPIEERPSARAPVARGVFGHETEAAD
                     PIEASPPPQPITGTHPKPATEGAVLVTGVCGRLGKLLARELHRSDRVVGVDRRPFDDR
                     PKDIVHHQVDMRRKKMKDIFRGRDIRAVVHLGILHDPRRSDIERHSWNIVAFQKLLDY
                     MTQYEVPKLVVLSGANVYGPQPNNPQFLTEDSPLLGAQHFGGMRDLVELDMLAQSFFW
                     KNPNTETVVLRPCHIVGRVHNAASNYLRLERPVMVMGFDPMMQVVHERDVVRAIQLAL
                     RPGVRGIFNIRGPGELPLSHLVRKAGARPRLVPSPLARVALDQLWKLRLSSFPSQELD
                     HLRYVCMVDDTRARAELGYEPVHSIDRALKDVRKARFQMA"
     gene            complement(6924..7865)
                     /locus_tag="VFG22_14915"
     CDS             complement(6924..7865)
                     /locus_tag="VFG22_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417586.1"
                     /translation="MANLRASSLLPIVVLLAGCPVYGPEPVQREVQVSCLSDFDCPSD
                     AFCDPDANSCVAYDFGICLTDGDCPVGSYCDRADGGCYIPGVALCTADPDCMSGFECD
                     FRDSCRPEVAGACLADGDCPSGSLCIENLCTPIAETCQFDFQCAAGFSCANNRCRMLC
                     GPETRCPSGTACEESLCQPVVGECVDSSECPDLATNCVEGICLLRCEEGCNPATELCD
                     SEGFCRPRTLPDPKAPAPFCRTDADCDGTVCVEGMCRTSCDSSAPDPGAICASYDGQL
                     PLCGADDLCYAESEVESECRVQPDCADGQDCVDGKCR"
     gene            complement(7911..8420)
                     /locus_tag="VFG22_14920"
     CDS             complement(7911..8420)
                     /locus_tag="VFG22_14920"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HET6417587.1"
                     /translation="MPQGLKLRAQPIAKPFHVVLVEPEIPPNTGAIARTCGATQSPLH
                     LVGPLGFRIDEHAVRRAGLDYWNLVQIHRHESFGAFEEAHSDVRIHFFSAGASRSYLQ
                     ADFEPGDALVFGRESVGLPSELLEDRQNVWAIPTLGPVRSLNLSNAVAIVLYEALRQN
                     RALDDTFLG"
     gene            8449..9441
                     /locus_tag="VFG22_14925"
     CDS             8449..9441
                     /locus_tag="VFG22_14925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417588.1"
                     /translation="MAKRGWFWVILGALVAVLVWRESRPEPSAALLQSVNVVALISPP
                     QGTGWDRIEMHPRVSEAILGTDPDRLRETMRDAGLEGIWVAVEPESPSGPELPLAARF
                     AAGGVVRGFRGKRFTSQGLLYVLDETSWPVVLSDRALARVARGVLEGSKPPPLDAFPE
                     ALTRPQAVEVLVLLTGGRGPRLWRSARAQSIAEALTTASLAARERWEERTETMGGPLG
                     ERLDQLDVEVALLIDDGTFDPTALSLIDTLVKPQHGVAYEQPARWRYLLPRATKDAGT
                     PTEAFRRLFRDNGLPDESFERADLRLYRFRMQTISIDQGSTGSRRTPAPSGSKD"
     gene            complement(9387..11522)
                     /locus_tag="VFG22_14930"
     CDS             complement(9387..11522)
                     /locus_tag="VFG22_14930"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6417589.1"
                     /translation="MDARTQTSFIAALLCFALAASVLLRTQRQRDRWLFGILATNTGL
                     WYVSTFMLGVVGRVPFWERFNLICGVLLPLSAVRFFGVMVPWETGRTRFLRRASVVAA
                     VVLLGAMLTPFYDHAIVVGGLLTYVTVFLSLALGYLYKLGFETDSRVEGARLRYIALV
                     GGFGGLFTLVEYLPYAGLDIPPVGTVLILVFLYALSRSITHYRLIDLYELAGRLGVLT
                     ALAFVLAFILWLMRLVSGEQLFLHVVVSAFVVLILFDPVRTKVQVWISQVFFHERFEL
                     ERTILALRRSLTNVLDVSELGQLLIDGLDASPRFTQGALYLLGPDARAFILKNHFGPK
                     PTDRVEMAPARPLLDRISKSGAAVLENAEREFEERRLLGDDREAETAHDIAVTMRALQ
                     SSLCLGLRGDSGDLYGFLTLRDDRLRDAFSREEVQLLAGLATQVAVTLENSQLYQQMK
                     ERDRLAALGEMAAGLAHEIRNPLGSIKASAQYLSDTFETPSEASEFLGIIVDEVDRLN
                     RVVSSFLDYARPGHTDPVPIHVNSAVQLTLQFLRPECDAAEVALHVAMEPDLPKVRID
                     IEHLRQVLINLVQNAVQAMTSGGEIFVETGIQDRFRIGGGARRWVQISVRDTGPGIAP
                     GLLANLFVPFVTTKQQGTGLGLAISQRIVSEAGGRIDVRSREGFGTTFVILLPAEGDP
                     QLDTFEAERAPAQSLDPDGAGVRLEPVEP"
     gene            11578..12396
                     /locus_tag="VFG22_14935"
     CDS             11578..12396
                     /locus_tag="VFG22_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HET6417590.1"
                     /translation="MSVQILDGAIGTELISRGLELTAPEWSARAVWDAPDLLAAVHAE
                     YAQAGATLHTANTFRTQPRALGPGWESALAAAVRIARESALPGQSVLGSMAPVEDCYR
                     PDLSPGAAARTEHRRIALALASAGCDRILCETFAHPSEALVAAEEAMATGLPVWVSLT
                     AGPFGQLLSPKALAEVARDLASSGIERVLLNCIAATRMDPYLEALSSLAAPIGVYANA
                     GREEEALGWGASGPRAAQAYADLAERWKNAGATVIGGCCGTGPLHIRAISERFA"
     gene            complement(12393..13817)
                     /locus_tag="VFG22_14940"
     CDS             complement(12393..13817)
                     /locus_tag="VFG22_14940"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6417591.1"
                     /translation="MSLEIIRWIANQLRLALLEEDESGAVTGNVHALESLGGKTPTDL
                     KSGAEQLLGRFESTDLTRLIESARGGTEASFAPRPGVQVLAIPAGADRAAVLITSQES
                     PLAASMQAKAAAADLAAGVSHEVANALSAIVGWAQVARERPDKAPPEEALALIEKSAQ
                     VARDATQDLLRMVRHSNHERACTDLSVVIRDVVRLLRPEAQSKRVQVRAEVEDSCWID
                     GKRSQLFSIVWNLAHNAVQMVPPGGEVSIHTSVHGKLIDLEVRDNGPGMSESQKVRAF
                     EPYYTTRSEGTGLGLAIVRGTVESLGGRIRLDTSPGRGAQFRIQLPEADMKRESQLVP
                     KPTRISRVMQPEQAERSRVLVVEDDEGVRGLISTTLALSGVSSTCVGTAAEALASSGP
                     FSAALIDLTLPDERGDILLGQLRKKGLVTRAGIMSGAPPPADADPDGQPERWLRKPFD
                     PSDLLLTVRQLIDSDAGTRNAAGS"
     gene            13943..14248
                     /gene="gatC"
                     /locus_tag="VFG22_14945"
     CDS             13943..14248
                     /gene="gatC"
                     /locus_tag="VFG22_14945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HET6417592.1"
                     /translation="MADAKITLEQVLHVAKLARLRLSEEEAQQMQEQLDAILGYMEGL
                     DELDVSDVPPTFHSIPMDAPLRPDVPERCSDRSETLGQAPESEAGGYAVPLVLEVDP"
     gene            14245..15711
                     /gene="gatA"
                     /locus_tag="VFG22_14950"
     CDS             14245..15711
                     /gene="gatA"
                     /locus_tag="VFG22_14950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HET6417593.1"
                     /translation="MSEFPWQGAFEIRTQIVDGSTSAVDVTRAYLDRAETLNPKLSCF
                     LTIDHEGSLAAASQVDRARAAGEALGPLAGVPIAVKDNLCTRGLRTTCASKILGNYIP
                     PYDAHAIEALRASGAVILGKTNLDEFAMGSSNENSAFANVHNPWALDRAPGGSSGGSA
                     CATAAGLAPIALGSDTGGSVRQPAAFCGITAIKPTYGRVSRYGLVAFASSLDQIGPMA
                     RTVREAAQLLEVIAGHDRRDATSADRETGAYIAACDRGVKGLRVGVVRDLVEGLQDAD
                     VRIGVEAALRVLQEQGATLVDVALPHAKHAVSVYYLIATAEASSNLARFDGIRYGLRV
                     RGEDLTETYTNTRAEGFGPEVRRRIMLGTYALSAGYYDAFYLKAQRVRTLIRRDYDTA
                     FESCDVVCTPTTPTPAFALGEKTGDPLEMYLADIFTLPPSLAGLPAISTPCGLSSAGL
                     PIGLQLVAPPFEEEVLCSASQAIEDGSGLAAAHPSALG"
     gene            complement(15715..17229)
                     /locus_tag="VFG22_14955"
     CDS             complement(15715..17229)
                     /locus_tag="VFG22_14955"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET6417594.1"
                     /translation="MPSEFGQRGKSPETFRVLVVDDSASIRRRVVQKLETMDFHITEA
                     SSGEEALALTLEQTFDLVVSDIEMGAITGVQLCRVFRGDPGMADVAFVLLTAAQETKT
                     RFWGRNAGADVYLAKEEMDEKLLPAIEKLMKGRTPRDSVRPELTGTPLERVSAVLDDH
                     LFDVVVQSEVRRLMDYVHDRIEFGNKVLELAAEVIGCPYLVLELLGPGGPTYAIRARG
                     PWPGGKSTAGLAALGVPEQNLGSAHTTVQPEPKFGCGENVVAGRSNTFDIRVRDEKLG
                     ALRVFGGTNVCAPVDVRSTELIADALGVVVKSLFLVEETQLLALTDGLTGLYNRRHAS
                     KRLEEEIARARRNSTGLCVAMCDVDHFKAINDEFGHSAGDRVLQQIAGSLTEYVRRND
                     IVSRWGGEEFLVIFSEIKLTAARIVAERLRGRLASTPKVDGGPDQISVSIGLAMLHAG
                     VTAEALIEQADQALYRAKARGRNRVEVAGEERRSSASRIVAAEDGDRTERAETK"
     gene            17332..18795
                     /gene="gatB"
                     /locus_tag="VFG22_14960"
     CDS             17332..18795
                     /gene="gatB"
                     /locus_tag="VFG22_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HET6417595.1"
                     /translation="MTSYEPVIGLEVHAQMRSRTKLFCGCPTDYGAPPNEHTCPVCLA
                     LPGALPVPNAKAIELAVQAGLALQCEVASHSVFARKNYFYPDLPKGYQISQFELPLNG
                     KGHLDIEIDGGTKRIGITRIHVEEDAAKNIHGTGSSAVTLVDFNRGGTPLVEIVSEPD
                     LRSADEAEAYLRKLRDLLMFIGVNDGNLEEGSFRCDANVSIRPVGQEELGTRAELKNI
                     NSFRFVKKAIEHEIARQEGVLQSGGTVVQETRTWSEAQSRTISMRGKEEAHDYRYFPD
                     PDLPPIVVTEKRIAELRAALPELPDAKRSRWQKELGITEYDAGVLSGHPEVAKYFEEA
                     TRLFIEMSGAKPSEAGKKVGNFVQSEVLRHTSTDGLEALFPVAPKAIAELLASVEDGT
                     INGKIAKKVFTSMVETGSAPKAIIEQQGLAQVTDTGAIEDAVRRILEASPSQVAQYKE
                     GKTSIIGYFVGQVMKATKGAANPKAVNEILGRLLDEQ"
     gene            18792..19868
                     /gene="mtnA"
                     /locus_tag="VFG22_14965"
     CDS             18792..19868
                     /gene="mtnA"
                     /locus_tag="VFG22_14965"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HET6417596.1"
                     /translation="MSAGLSRAPLSGAEWFAVEFDPSTAELVMLDQRLLPGEVVFRRY
                     RKPDEVADAIREMVVRGAPAIGIAAAYALALVARNEAGDAKMFLVANGVAGRILNGTR
                     PTAVNLGWAIARMSRKAGEVCELPPELRYQGMLEAAESIHREDVAACRRIGELGAADV
                     PEGATILTHCNAGALATGGFGTALGVIRAAHAAGKNVRVLASETRPYLQGARLTAWEL
                     HRDGIPVEVITDNMAGHFFQMGDIQFVVVGSDRIAANSDIANKIGTYSLAVLANQHGV
                     PFTVAAPWSTVDIRTRSGADIPIEERSREEVAHVAGVTIVADGIPCRHPAFDVTPARL
                     IDAIYTERGVIRPKIGERPDVLRG"
BASE COUNT         3615 a         6347 c         6338 g         3661 t
ORIGIN      
        1 ggagggtgat cgtgtcgatc ccagtcagca cctcgtctag atcgaagtga aggtcgggca
       61 agcgggccag cgccttcgtc cagtactcgc gtatcgcggg cttgccccgt agcgtcccgc
      121 tcggctcacc cacggcgtcg atgatcacag gcgacgagag ctcgaagtcg tcagtgtagt
      181 gtaccaagac cgcgtcgagg tcgcgccggt tccacgcgtc gatccagtcg ctggcaaaag
      241 cgcgcgcgaa ctcctgctcc actcgtggca caaccgcctc cgtcagtcga tggcttcgaa
      301 gaggcccgcc gcgcccatgc cgccgccgat gcacatcgtg accaccgcgt acttgccgcc
      361 tcgacgcttg agctcgttga gtgccgtcgc cgtgagcttg gcgcccgtgc agcccaaggg
      421 atggccgaga gcgatggccc cgccgttgac gttgagcttg tcgttcggga tgccgagctc
      481 cttctggaca tagacggcct gactcgcgaa ggcctcgttg acctcgaaga tgtcgatgtc
      541 gtcgatgctg aggtcgttct tgcgaaggag cttttggacg gccgggagcg ggccgatgcc
      601 catgatcgac gggtcgacgc cgaccgtcac gaacgacttg aagatcgcca tgggtgcgat
      661 gccgagcttc tcggcccgct ttttcttcat cacgatggcc gcggccgctc cgtccgagag
      721 gggcgaggag ttgcccgcgg tgacgctgcc cttggcggca aacaccggcc gcaattgcga
      781 gagcgcttcc accgtcgtgc cggggcgtgg catctcgtcg accttgaact cgaacatctg
      841 acgttcgccg ttcttgtcga acgtcacgcc atgcaccggg acgatttcgt cgacgaaggc
      901 cccccgcttg atggcggcga gcgccttctc gtggctccag gccgcgaact cgtcctgctg
      961 cgtacgactg atcttgaatc ggttggccac gttctccgcg gtgattccca tcggcgtgta
     1021 cgtctcgggg tacttctcca tgacctcggg cgaggcgctc ggcttgttcc cggtcatcgg
     1081 gaccatggtc atcgactcca tgccgcctgc gatgacgacg tcgtcgaagc ccgccttgac
     1141 ggagcccgcg gccaaggcga tggcctggag cccgctcgag cagaagcggt tgatcgtcat
     1201 gcccgagctc tcctcgggga gacccccgag tctgccgacc atgcgcgcca cgttcatgcc
     1261 ttgctcccct tcggggaacg cgcagccgag aaccaggtcc tcgaccgtgg ccgggtcgac
     1321 cccggggttg cgcgccatca aagcgcgaat cacctcaccc gcgagctcat ccgggcggcg
     1381 attggccagg gacccttttt tggcgcgccc aacagcgctt cgaaccgcat ctacgacgac
     1441 aatcttaccc atgtctttac tcctgattcc tagttggctc ggcgtcccgg gttggcctag
     1501 ttcctcaacg gcttgttggt ggtgagcatg tgctgcatgc gctcctggct ctttggctcg
     1561 ccacaaaggc tcacgaacgc ctcgcgctcg agctcgagca tttgctcctc ggtgacctcg
     1621 tgggcgttgc ccgacgggcc gccacaaagc acggacgcga ccttgcgagc gatcagcgcg
     1681 tcgtgcgcgc ttgcgaaacc acccgcttcg agcgagtcga tcatcatctc gagcgtcgcg
     1741 atgccgctct cgccaggcag ccggtacgca cgtcgaaccg gcggatggta gcccgcttcg
     1801 gccatgccga tcgcgcggcc cttggcctcg gtcagcaagc gcgccttgtc gaacgagatc
     1861 ccgtcagact ttcggaagta tccgaactcc cgcgcctctc ctgcaccggt ggccactctg
     1921 gccatggcga tgttctggaa ggtgcgcgac acgaagggca gggtgtcgac gtcggttccg
     1981 tcgggaatcc cctctagcgc ccgccagagc atgttcatgt tgcctgcgcc gcctggaagc
     2041 agaccgacgc ccacttccac gagaccaacg taggtctcag acgccgcttg caccgcgtcc
     2101 gcgcccatgc agacctcgaa gccgccgcca acggtcatcc caaacggggc cgccacgacc
     2161 ggtatggtcg agtacttcat gcgctgaagg ccaccctgca agccgctgat cgtcgcgcgg
     2221 agctgatccc aggccttctg ctgcgccgcc atgaccaccg caaagaggtt ggcgccgacg
     2281 cagaagtggt cgccgtcgtt gtagatcacc atcccgcgga aatcgctttc ggcgatctcg
     2341 accgccttcg tgatgccctc gatgacggtg tcgtcgatgc tgttggcctt cgtggtgaag
     2401 gtcagaccga ggatcccgtc gccgagatcc caggcttcgc ttccgcggtt gctgaagacc
     2461 ggtgcgtcac cctggcgcat gatcgtgagg ctcgcgttgc gcgggtcgat ctcccgggct
     2521 tcgtactcgc cggtgaccag gctgaacacc cgcccatcgt cggtgtagaa gctctgcgcg
     2581 cccgactccg ccatccgatc gatcgacgcg ggaatcgaga gtccgtcggc cttcatgcgc
     2641 tcgtaagccg ccttgaagcc gatcgcgtcc caggcctcga agggaccgag gtcccagttg
     2701 tagccccact tcatcgcgtt atcgaccgct tccacctcgt cggcgatctc gccgatcatc
     2761 gtggcggagt aggccagcgt gcgcgagaga acgttccagg cgaacttgcc cgcagggccg
     2821 tcgtttgcga ccagcgcctt cacacggtct gcgggtgagc ccttgagtcc cttgcagaac
     2881 ttcttgatgt cctcgtcgcc cgctttcggg cggtattcga gcttgaccgg gtcgaaggtc
     2941 tcgatgcctt cgcgtgtctt cttgtagaag cctccgcggg tcttgttgcc gaggatcttc
     3001 tgttcgacca tcgcgaccag gaagtgcggc ggcttgaaga cgtcgcgctc cggatcgtcg
     3061 accagggcct cgtagcagtt gtcggacacg tgcttgaacg tgtcgagacc gaccatgtcc
     3121 gcggtccgga agctcgcgct cttcggatga cccatcggcg gcccggtgat cgcgtccacg
     3181 tcctccggcg tgaggcccgc gtcgatcatc tcgttcatcg tgagagacat ggagtagcaa
     3241 ccgatgcggt tgccgacgaa gttcggcgtg tccttgccga tgacgatgcc cttgcctagc
     3301 tggttctcac cgaagacctt catgcgctcc acggtcgctg ggtcggtttc ctcgcccgcc
     3361 acgagctcca ggagcttcat gtaacggacc gggttgaaga agtgcatcac gaggaagttc
     3421 ttgcggaatg cctcgccgcg gccgtgcagc atgtccgcga tccgaagtcc ggaagtgttc
     3481 gacgcgacga ccgttccctc gtcgagagtc ttcttgagct tctcaaagag gctgcgcttg
     3541 atgtcgatgt tctcgatgat cgcctcgata acgaggtcgc accccttgag caggccgatg
     3601 tcgtcttcca ggttgccgac ggtcacgcgc ttggcgtagt tcgggtgaaa gaacgcagcc
     3661 ggcttcgcct tgagcgcgcc ctgcaggccc cccgccgcaa attgattgcg cttcgcaggg
     3721 ttcttcttct cttcatcgct cagccccggg ggcacgatgt cgagcatcac cacgtcgacc
     3781 ccggcgttgg caaagtgcgc catgatgccg ctgcccataa cgcccgctcc gatcacacct
     3841 gctcgccgta ctggtttcgt catacttctc tcctgaggtt cgggcggggg aactttccgc
     3901 aagacgcgct cgaaagcaag ccgttacgcc aatcgcccag atagtgccgc tatttgtaag
     3961 gagtagcggt gagagctgcg accaactcgt cgtgaatgag accgttgctc gcgatcgcgc
     4021 cgccgccgta gattgtctct tcacctcgga aggaggtgaa tcttcctccc gcctcgcgga
     4081 tcagtaccgc cggcgcgcag atgtcgtagg gctgaacacc cggatcgacc atggcatccg
     4141 agcggccgag aaggatctgc cggtatccgt cgaagtcagg gcagccgcgc gcgatatcac
     4201 aagcgtccga taagcgaagg agcgcctcgc caactcccgc gatcgtgaac tgttgaaggc
     4261 cgccatgggt gaccgtcgcg tctgcgagag gagaaacctt cgacacgcga acggtgttcc
     4321 cgttgtgcgt tgcaccgccg ccctgcaccg ccacgaagaa atcgccgtcg gccggcatgt
     4381 aggcgattcc aacgaccgga acgccatccg cctcgactcc catgagcacg gaccaagtcg
     4441 gccaaccgcg cacgaaagcg cgcgttccgt cgatcggatc aacgatgaaa cggatccccg
     4501 tgctgccttt gtgcgttcct gtttcctcgc cgaggaaccc agcgtccgga tgtgcggcga
     4561 gcacgtggtc gcgaatgatc tcctcggcct cacgatcggc gatcgtgacg gggctgcgat
     4621 cgggtttctt ctccggcacc acaccctgtc ggaaatgttt caacgcggca tcccctgcgc
     4681 ggcgcgtgac gtcttccaac gcgcgcaacc cgccgggtcc accgatacga cggatgtcga
     4741 agccgtggct catgcgcgaa gcatgacctg tctaggcgct tccgtgaagt gacagtgtcg
     4801 acaggttgta acgtggattc cgattgacgc gtgcggcttc tgtctacacg atggctgcat
     4861 ggcgcaaggg ggctcgatcg tgccattcgc cggctggcgc cggagtcggt tgcgggggga
     4921 atcccctctg cggcaagcgg tgcgatccgt cggcgatcgt gtcgtgcgat tcgccgaaga
     4981 ggtcgttgga caagacctcg atgcgcggat cgccggaatc agcacccacc tcaacgaggt
     5041 cggccacgat ccgttcggat tcgacccgga gacgagccgc tatgcgctcg cactggcggc
     5101 gcttttgcat cgccgctact tccggacgga ggtctttggt atcgagcgcg tgccgcaggg
     5161 cagggtgctc atcgtcgcga atcactcggg ccagctcccc gtggacgcgg tcatgatcgc
     5221 gagcgcgctg atgctcgacg ccgatccgcc acgctttcct cgaagcatgg tcgaaacttg
     5281 gaccgccgag cttcccttca tttccaccct ctatccgcga ctcggccagg tgatcggctc
     5341 accggagaac gcgaggcgcc tccttcgaca ggaagaaacc ctgatcgtct tccccgaggg
     5401 agcccgtggc atcagcaaga ccttcgacca gcggtatcag ctcaccgact ttggactcgg
     5461 gttcatccgc ttggctctcg agaccggcac tcccatcgtt ccggtcgccg tcataggcgg
     5521 cgaggagcag tatccttcga tcgcagactt caagcctctc gcacgtctgc tgaagatgcc
     5581 tgcgttccct gtgatgcctc aggtcttctt tgggatgatt gccccgctgc cgacgaagta
     5641 ccggctctac ttcggagagc ccttcctttt tggtggcgat cccgacgacg acgaccgagc
     5701 gatcgaagag aaagtttggc tcgtgaaaag cacgattcaa agcatggtga accgcggctt
     5761 gcgcgaacgt aaaggtgtgt tcatctaagc gagatccggc ccatgcccaa tcctcaagcc
     5821 acaaaccgaa acaagccgag cgcgcgccgc gggcgcaatc cgatcgagga gcgtccttcg
     5881 gctagggcgc cggtagcaag gggtgttttt ggtcacgaga ccgaggccgc tgacccaatc
     5941 gaggcctcgc caccgcccca gcccatcacc ggcactcacc cgaaacccgc gacggagggc
     6001 gccgtgctgg tcaccggagt ctgcggccgc ctaggcaagc tactcgcccg ggagctccat
     6061 cgaagcgatc gcgtggtggg cgtcgatcgg cggccctttg acgaccggcc caaggacatc
     6121 gtccatcacc aggtcgacat gcggcgcaag aagatgaaag acatcttccg gggtcgcgac
     6181 attcgagccg tggtccacct cggaatcctc cacgatccga ggcgaagcga catcgaacgg
     6241 cattcctgga acatcgtcgc cttccaaaag ctcctcgact acatgaccca gtacgaggtg
     6301 cccaagctcg tcgtgctgtc cggggccaac gtctacggtc cgcagccgaa caatcctcag
     6361 ttcctcacgg aggattcacc tttgctcggc gcccagcatt ttgggggcat gcgcgatctc
     6421 gtggagctcg acatgctcgc ccagagcttc ttctggaaga accccaatac cgagacggtg
     6481 gtgctgcgac cctgtcacat cgtcgggcgt gttcacaacg cagcgagcaa ctatctgagg
     6541 ctcgagcgtc ccgtgatggt catgggcttc gatcccatga tgcaggtcgt gcacgaacga
     6601 gacgtcgtcc gcgcgatcca actcgcgctg cgccctggcg ttcgtggcat cttcaacatc
     6661 cgcggccctg gcgagctgcc gctgtcgcac ttggtacgga aggcgggtgc gcggcctcgc
     6721 ctcgttcctt cgcccctggc aagagtggcg ctcgaccagc tttggaagct gcggctcagc
     6781 tccttcccgt cgcaggagct cgaccacctc cgctacgtct gcatggtcga cgacacacgg
     6841 gcccgcgccg agttggggta cgagcccgtg cattcgatcg accgcgccct caaggacgtg
     6901 cggaaggcgc gcttccagat ggcttagcgg cacttgccgt cgacgcaatc ttgcccgtct
     6961 gcgcagtccg gctgcacccg gcactcgctc tcgacttcgc tctctgcgta gcaaaggtcg
     7021 tctgccccgc agaggggaag ctgcccgtcg taggaggcgc agatcgcacc gggatccggt
     7081 gcgctgctgt cgcatgaggt ccggcacatc ccctccacgc acacagtgcc gtcgcagtct
     7141 gcgtcggtgc ggcagaaggg agctggcgcc ttggggtcgg gaagagtgcg aggacggcag
     7201 aaaccctccg agtcgcagag ctcggtggcg gggttgcagc cctcttcgca acggagcagg
     7261 cagatccctt cgacgcaatt ggtcgctaga tccgggcatt cgctgctgtc gacgcattcc
     7321 ccgacgacgg gctggcaaag actctcctcg caggcggttc cggatggaca tcgcgtctcc
     7381 ggcccgcaga gcatccggca gcgattgttg gcgcagctga accctgcagc gcactggaag
     7441 tcgaactggc aggtctcggc gatcggcgtg cagaggttct cgatgcaaag cgatccgctc
     7501 gggcaatcgc catcggccag gcatgccccc gcaacctcgg gccggcaact gtcgcggaag
     7561 tcgcattcga atccagacat gcagtcaggg tcagccgtgc acagagcgac tcccgggatg
     7621 tagcagcctc cgtcagcacg atcacagtag ctgccaaccg ggcaatcgcc gtcggtgaga
     7681 cagatcccga aatcatatgc aacacaggag tttgcgtcgg gatcgcagaa cgcatctgaa
     7741 ggacaatcga agtcgctcag gcaggaaacc tggacctctc gctgcacggg ttcaggcccg
     7801 tagacggggc agccggcgag gagcactacg attgggagaa gggagctcgc gcgtagattg
     7861 gccatgtcac tcggacgcag ccccgccggg ggtattcaag agaaatggcg ctagcccaga
     7921 aaggtgtcat cgagggcacg gttctggcgc aatgcctcgt acaagacaat ggcgacggcg
     7981 ttgctcaggt tgaggctgcg gaccgggccg agcgtgggga tcgcccagac gttctggcgg
     8041 tcctcgagca actcgctcgg gagccccacc gactcccggc cgaagacgag agcgtcgccc
     8101 ggctcgaaat ccgcttgaag ataggacctc gatgcgcctg cggaaaagaa atgaattcgg
     8161 acatctgagt gagcttcttc aaaagctcca aaggattcat gtcgatggat ctggaccagg
     8221 ttccagtagt ccagacccgc gcgccgcacc gcatgctcat cgatccggaa gcctaagggc
     8281 ccgaccaagt gcagcggtga ttgggtcgct ccgcaggtcc gggcgatcgc gccggtgttg
     8341 ggcgggatct ccggctcgac caagaccacg tggaaaggct tcgcgatcgg ctgagctcgg
     8401 agcttgaggc cttggggcat gcccgttctt acgatacagc gtagcgacat ggcaaagcgg
     8461 ggttggttct gggtcatctt gggcgcgctc gtggcggtgc tggtctggcg cgagagccgg
     8521 cccgagccct ccgctgcgct gctgcaatcg gtgaacgtcg tcgcactcat ctccccgccc
     8581 cagggcactg gctgggaccg gatcgagatg catcctcgag tgtcggaagc gattctcggc
     8641 accgaccccg atcgacttcg ggagacgatg cgagatgccg ggctcgaggg catttgggtt
     8701 gccgtcgagc cggaatcgcc ctccgggccc gagcttccgc tcgcggctcg ctttgctgcc
     8761 ggcggcgtgg tgcgcgggtt tcgaggaaag cggttcacct cccaagggct cctctacgtc
     8821 ctcgacgaga cgagctggcc ggtggttctg tcggaccgcg cgctcgcgcg ggtcgcacgc
     8881 ggagttctcg aaggaagcaa gccccctcca ctcgacgcgt ttcccgaagc actcactcgg
     8941 ccacaggcag tcgaagtcct cgtcttgctc acgggaggtc gaggtccacg tctttggcgt
     9001 tcggcgcgtg cgcaatcgat cgcggaagcg ctgacgacgg cgtcgcttgc cgcgcgcgag
     9061 cgatgggagg agcggaccga aacgatgggc ggacccctcg gagagcgtct cgatcagctc
     9121 gacgtcgagg tcgcgttgct catcgacgac ggcacgttcg atccgactgc gctctcgttg
     9181 atcgacacgc tagtgaaacc acagcacggc gtggcctacg agcagcctgc gcgttggcgc
     9241 tatctgctgc cgcgcgcgac caaggacgcc ggaaccccga ccgaagcgtt tcgccgactg
     9301 ttccgggaca acggtctccc tgacgagagc ttcgagcgag cggacctccg actttatcgc
     9361 ttccggatgc agaccatttc gatcgatcag ggctcgacgg gttcgaggcg caccccagct
     9421 ccatcggggt ccaaagactg agcgggcgcg cgttcggcct cgaacgtgtc gagctgagga
     9481 tcgccttcgg ctggcagcag aatgacgaaa gtggtgccga agccttcgcg actccgaacg
     9541 tcgattcggc ctccggcctc ggaaacgatg cgttgtgaga tcgcgagccc gagcccggtg
     9601 ccttgctgct tggtggtcac gaagggaacg aagaggttgg cgagcagtcc gggcgcgatt
     9661 cctggcccgg tgtcgcgcac gctgatctgg acccagcgcc gggcgccgcc gccgatccga
     9721 aagcggtcct ggatgcccgt ttcgacgaag atctctccac cactcgtcat cgcttgcacg
     9781 gcgttctgaa cgagattgat cagcacctgc cggagatgct cgatgtcgat tcgaaccttg
     9841 ggcaggtccg gttccatcgc gacatgaagg gcgacttcgg cggcatcgca ctccggacgg
     9901 aggaactgca gcgtgagttg aaccgcggaa ttgacatgga tcggcactgg gtccgtgtgg
     9961 ccaggccgcg cgtagtcgag gaacgagctc acgactcgat tcaatcggtc gacctcgtcg
    10021 acgatgatcc ccaggaactc gcttgcttcg ctcggcgttt cgaaggtatc cgacaggtac
    10081 tgagcgctcg ctttgatcga gccaagtggg tttcggatct cgtgcgcaag gcccgcggcc
    10141 atctcaccga gagccgcgag tcgatccctt tccttcatct gctggtagag ctgcgagttc
    10201 tccagggtga ccgcgacttg agtggccagg cccgcaagca attggacctc ctcccgcgag
    10261 aacgcgtcgc gcaagcggtc gtcgcgaaga gtcagaaagc cgtagagatc ccccgagtcg
    10321 ccccggagtc cgaggcatag ggaagattga agcgcacgca tggtgaccgc gatgtcgtgt
    10381 gcggtctccg cttctcggtc gtcgcccaag agccgccgct cctcgaactc gcgctctgcg
    10441 ttctccaaaa ctgccgcgcc acttttcgag atgcgatcga gcagcgggcg cgccggcgcc
    10501 atttcgacac gatccgtggg tttgggaccg aagtggttct tcaagatgaa cgcgcgcgca
    10561 tccgggccga gcaagtacag cgcgccctgg gtgaatcggg gcgaggcatc gaggccgtcg
    10621 atcaagagct gaccgagctc ggagacgtcg aggacgttgg tgagcgatct gcgcagggcg
    10681 agaatggtgc gttcgagctc gaaacgctcg tggaagaata cctgcgaaat ccaaacctgc
    10741 accttggttc gcactgggtc gaagagaatg aggaccacga acgcggatac gaccacgtga
    10801 agaaagagct gctcaccgga gaccagacgc atcagccaca agatgaacgc cagcacgaac
    10861 gcgagggcgg tcagcacgcc tagacgtccg gcgagctcgt agagatcgat cagccggtag
    10921 tgggtgatcg atcgcgagag ggcgtagagg aagaccagga tcaggacggt acccaccggg
    10981 ggaatgtcga ggcctgcgta agggagatac tccaccaaag tgaagagccc gccaaagcca
    11041 ccgacgagcg cgatgtagcg aagacgggcg ccttcgactc gggagtcggt ttcgaaaccc
    11101 agtttgtaga ggtatccgag agccagggag aggaagaccg tcacgtaggt gaggagcccg
    11161 ccaacgacga tcgcgtggtc atagaacggc gtcagcatgg cgccgagcaa caccacggcg
    11221 gctacgaccg acgcgcggcg gaggaaacga gtgcggccgg tctcccacgg gaccatcacg
    11281 ccgaagaacc gaactgcgga gagcggcaag aggacgccgc aaatcaagtt gaatcgctcc
    11341 cagaacggaa cgcggccaac cacaccgagc atgaaggtgc tcacgtacca gagccccgta
    11401 tttgtcgcga ggatgccaaa aagccatcgg tcacgttgac gttgcgtacg taaaaggacg
    11461 ctcgcggcga gcgcgaagca gagcagcgca gcgatgaacg aggtttgtgt tcgcgcgtcc
    11521 accggcggag cgtaacagct ccctttcgtc tggtcagttg ctagagtgcg tctcgagatg
    11581 agcgtgcaga tcctagatgg ggcgatcggt accgagttga tttcacgcgg cttggagctc
    11641 acggcacccg agtggagcgc gcgggcggtc tgggatgcgc ccgatctgct cgctgcggtg
    11701 cacgccgagt acgcccaggc aggcgcaact cttcacaccg ccaacacgtt tcggacgcag
    11761 ccacgtgctc ttgggccggg ctgggaatcg gcgctcgcgg cagccgttcg catcgcacgg
    11821 gagtcggcgc tgcccgggca gagcgtgctc gggagcatgg cgcccgtaga agattgctac
    11881 cggcccgatc tcagtccggg agctgcggcg cggaccgaac atcgcaggat agctttggcg
    11941 ctcgccagcg caggttgcga ccggattctc tgtgagactt ttgcccaccc cagcgaggct
    12001 ttggtcgctg cggaagaggc aatggccacc ggcttgccgg tgtgggtgtc cttgaccgcc
    12061 gggccgttcg gccaactcct ctcaccgaag gcgcttgccg aggtcgccag agacctggca
    12121 tcgagcggta tcgaacgggt gctgctcaac tgcatcgccg cgacacgaat ggatccgtat
    12181 ctcgaggcct tgagctcgct tgccgcgccg attggggtgt acgccaatgc cggacgagaa
    12241 gaagaagccc tcggctgggg cgcgagcggt ccccgcgccg cgcaagccta tgcagacctc
    12301 gcggaacgct ggaagaacgc aggcgcaacc gtcatcggtg gctgctgcgg aacgggcccg
    12361 cttcacatcc gagccatctc ggaacggttt gcctaagagc cggccgcgtt gcgggttccc
    12421 gcgtcggagt cgatcaactg tcgcaccgtg agaaggagat ccgagggatc aaaaggcttg
    12481 cgaagccagc gctcgggctg gccgtcgggg tccgcatccg ccggtggggg agccccgctc
    12541 atgatcccgg cgcgggtgac caaacccttc ttacggagtt gcccgagcag gatgtcgccg
    12601 cgctcatcgg ggagcgtgag atcgatcagt gcggctgaaa acggccctga agaagcaagt
    12661 gcttcggcag ccgtcccgac gcatgtcgag gaaacgccgc tgagcgcgag cgttgtcgag
    12721 atgagacccc gcacgccttc gtcatcttcg acgacgagca cgcgtgaacg ctctgcttgc
    12781 tccggttgca tgacgcggct gatccgagtg ggcttgggca ccaactggga ttcccgcttc
    12841 atgtcagcct ccggaagctg gattcgaaat tgcgcacctc ggcccggtga ggtgtcgagc
    12901 cggatccggc cgccaagcga ttccaccgtg cctcgtacga tcgccaagcc gaggccggtg
    12961 ccttccgagc gggtcgtgta atagggttcg aaggcgcgca ccttctggga ctccgacatg
    13021 ccaggtccgt tgtctcgcac ttcgaggtcg atcaacttcc cgtggacgct ggtgtgaatc
    13081 gaaacctctc cgcctggagg aaccatctgc accgcgttgt gtgcgaggtt ccagacgatt
    13141 gagaaaagtt gcgatcgctt cccatcgatc cagcaggagt cctcgacctc ggcgcgaacc
    13201 tggacgcgct tggactgcgc ttcgggccgc agcagccgta cgacgtcgcg gatgaccacc
    13261 gaaagatcgg tgcaggctcg ctcgtgattg ctgtgtcgca ccatccgcag aagatcttgc
    13321 gtggcatcgc gggcgacctg cgcgctcttt tcgatcaggg caagagcctc ctcgggcggg
    13381 gccttgtccg ggcgctcccg agcgacctgc gcccagccga cgatggcgct gagcgcgttt
    13441 gcgacctcgt gggacacgcc ggcggcgaga tctgcggcag cagccttggc ctgcatgctt
    13501 gcggcgagcg ggctctcttg cgaagtgatc aagactgcgg cgcggtcagc gccagcgggg
    13561 atcgccaaga cctgcacacc gggtcgcggc gcgaaagacg cctcggttcc tccgcgtgcg
    13621 ctctcgatca gccgggtgag gtccgtcgac tcaaaacgac cgagcagttg ctccgcaccg
    13681 gacttcaggt ccgtcggcgt cttgccgcca agcgactcga gcgcgtgcac gttgccagtc
    13741 acggcgccgc tctcgtcttc ttcgaggaga gccagtctca gttggttagc aatccaacga
    13801 attatttcta atgacaccgc aaacgtccgt ttcaagcaaa atgtaagctg cagtggcctg
    13861 cttcgtcaat cgaaaggcat gttttggtta gtgaatacgc agacctcgcg ccaaagaacg
    13921 gatctgctac cttcggcgcc ccatggcgga cgcgaaaatc accctcgagc aggttttgca
    13981 cgtcgcgaag ctggccaggc tgaggctgtc cgaggaggag gcgcagcaga tgcaggagca
    14041 gctggatgcg atcctcggct acatggaggg gctcgacgag ctcgacgtct cggacgttcc
    14101 gccgaccttt cattcgatcc cgatggacgc cccgctgagg cccgatgttc cggagcgctg
    14161 cagcgaccgc tcggagaccc ttgggcaagc gcccgaaagc gaggccggtg gatacgcggt
    14221 gccgctggtc ctcgaggtcg acccatgagc gagttcccct ggcaaggcgc cttcgagatt
    14281 cgcacgcaga tcgtggacgg cagcacgagc gcggtggacg tgacgcgggc gtatctcgac
    14341 cgcgccgaaa cactgaaccc gaagctttcg tgctttctga cgatcgacca tgaaggctcg
    14401 ctcgctgctg catcgcaggt cgaccgtgca cgcgcagccg gtgaagcgct cggcccgctg
    14461 gcgggtgtgc ccatcgccgt gaaggacaac ctctgcactc gcggcctgcg caccacctgc
    14521 gcttcgaaga tcttgggaaa ctacattccg ccctacgacg cgcatgcaat cgaagcctta
    14581 cgtgcatcgg gcgcggtgat cctcgggaag accaatctcg acgagtttgc gatgggctcg
    14641 tcgaacgaga actccgcttt cgcgaacgtg cacaatccct gggcgctcga tcgcgcgccc
    14701 ggcggctcgt cgggcggttc agcctgcgcc accgctgcag ggctagctcc gatcgcgttg
    14761 ggaagcgaca ccggcggctc ggtccgacag cctgccgcgt tttgcggaat cactgcgatc
    14821 aaaccgacgt acgggagagt gtcgcgctat ggcctcgtcg cattcgcctc gtcactcgac
    14881 cagattggac cgatggcgcg tacggttcgg gaagccgcgc agctcttgga ggtcatcgct
    14941 ggccatgatc ggcgtgacgc gacctccgct gaccgtgaaa ccggagctta catcgccgcc
    15001 tgtgatcgcg gcgtgaaggg gctcagggtg ggcgtggttc gcgatctcgt cgaaggactt
    15061 caggatgcgg acgttcgtat tggagtagag gccgcactgc gcgtgctcca agagcagggc
    15121 gcgaccctgg tggacgtggc gcttccgcac gccaagcacg cggtttcggt ctactatctc
    15181 atcgccacgg cagaggcgtc cagcaatctc gcgcgcttcg acggcattcg ctacggcctt
    15241 cgggtgcgcg gtgaagacct caccgagacg tataccaata cgcgcgccga gggctttggc
    15301 cccgaagtca ggcgccggat catgctcggg acatacgcgc tctcggcggg gtactacgac
    15361 gccttctatc tcaaggcgca aagagttcgc accctgatcc gccgcgacta cgacaccgcg
    15421 ttcgaaagtt gtgacgtcgt ctgcacgccc acgacgccga ccccggcgtt cgccttgggc
    15481 gagaagacgg gcgacccgct cgaaatgtat ctcgccgaca tcttcacgct tcctccgagt
    15541 cttgccggcc tgcccgcgat cagcacgcct tgtggcctct cgtcagcggg gctgcccatt
    15601 gggcttcagc tcgtggcgcc gccgttcgag gaagaggttc tttgcagcgc ctcgcaggcc
    15661 atcgaagacg gttctggcct ggcggccgcg catcccagcg cactcggctg aaggctactt
    15721 ggtctcggcg cgctcggtcc ggtcgccatc ctccgcggcg acgatcctcg atgcgctgct
    15781 gcgacgctct tcgccggcga cctcgacgcg gttccgtcct ctcgccttcg ctcggtagag
    15841 cgcctggtct gcctgctcga tcagcgcctc ggcggtgact ccggcgtgca gcatggccag
    15901 cccgatgctc acgctgattt gatcagggcc accatcgacc ttcggggtgc ttgcgagccg
    15961 tcctcgaagc cgctcggcga cgattcgcgc ggctgtgagc ttgatctcgg agaagatcac
    16021 cagaaactcc tcgccacccc aacgggaaac gatgtcgttg cgcctgacgt actcggtgag
    16081 cgagcctgcg atctgttgca gcacacggtc cccggcgctg tgtccgaatt cgtcgttgat
    16141 cgccttgaag tgatcgacat cgcacatcgc gacacagagg ccggtgctgt ttcggcgggc
    16201 gcgcgcgatc tcctcctcga gtcgcttgct tgcgtgccga cggttgtaga gccccgtgag
    16261 cccgtccgtg agcgcgagta actgcgtttc ttcgacgaga aagagcgact tcaccaccac
    16321 gcccagggca tcggcgatga gctccgtgct tcgcacgtct accggagcgc acacattggt
    16381 accgccgaat acccgcaaag ctccgagctt ctcgtccctc acacggatgt cgaaggtgtt
    16441 cgaacgaccg gctactacgt tctcaccgca gccgaatttc ggttcgggct ggaccgtcgt
    16501 atgggcggat ccgaggttct gctccgggac cccgagcgcc gcgagaccgg ccgtcgactt
    16561 gccgcccggc cagggtcctc tggcgcgaat cgcataggtt gggccgccag gcccgagaag
    16621 ctccaagacg agatacgggc aaccgatgac ctcggctgcg agctcgagca ccttgttgcc
    16681 gaactcgatc cggtcatgga cgtagtccat caaacgacgg acttcggact gcaccaccac
    16741 gtcgaagaga tggtcgtcca gcacggcgct gactcgttcg agcggggtac cggtcagctc
    16801 ggggcgaacg ctgtctcgag gcgtgcggcc tttcatgagc ttttcgatcg ccggcaagag
    16861 cttctcgtcc atctcttcct ttgcgagata gacgtccgcg ccggcattgc gaccccagaa
    16921 ccgtgtcttg gtctcctggg cggccgtgag cagaacgaag gccacgtcgg ccatgcccgg
    16981 atcaccccga aagactcggc agagttggac gcctgtgatc gcacccattt cgatgtcgct
    17041 cacgacgagg tcgaaggttt gttctagggt cagagcgagg gcttcttccc cggagctcgc
    17101 ctcggtgatg tggaaatcca tcgtctcgag cttctgcacg acacggcgtc gaatcgacgc
    17161 actatcgtcg accaccagaa cccgaaacgt ctcgggcgat tttcccctct gcccaaactc
    17221 cgaaggcatt tgtacccttc tccatgatgg gtaaatccct agtgaaatgc caatgtatct
    17281 ctggcttgga cgccgtagcc ttctgaccct ggtgcgctat aacccgccga catgaccagc
    17341 tatgagcccg tgattggact cgaagtgcac gcgcagatgc gttcacgaac caagctcttt
    17401 tgtgggtgtc ccaccgacta tggggccccg cccaatgagc acacatgtcc agtgtgcctg
    17461 gcccttccgg gggctctccc cgtgccgaac gcgaaggcga tcgagctcgc ggtgcaagcc
    17521 ggcctggcgc tccaatgtga ggtcgcctca catagcgtgt tcgcgcgaaa gaactacttc
    17581 tatccggatc ttcccaaggg ctaccagatc tcgcagttcg agcttccgct gaacggcaag
    17641 ggtcatctcg acatcgagat cgatggcggt accaagcgga ttgggattac ccgcatccac
    17701 gtcgaggagg acgcggccaa gaacattcac ggcacgggtt cgagcgcggt caccctggtg
    17761 gatttcaatc gcggtggcac tccgttggtt gaaatcgtga gtgagccaga cctgcgaagc
    17821 gccgatgagg ccgaggcgta tctccgaaag cttcgcgacc tgctgatgtt catcggcgtc
    17881 aacgatggca acctggagga gggctctttt cgctgcgacg ccaatgtgtc gattcgcccc
    17941 gtcggccagg aggagcttgg aacccgcgcc gagctcaaga acatcaactc gtttcgtttc
    18001 gtgaagaaag cgattgagca cgagattgcg cgtcaggaag gcgtcctgca aagcggcggc
    18061 acggtggttc aggaaacccg cacctggagc gaggcacaga gccggacgat ttccatgcgc
    18121 ggcaaagaag aagcgcacga ctatcgctac tttccagatc cggacttgcc accgatcgtg
    18181 gtgaccgaaa agcgaatcgc cgagcttcgt gcggcgcttc ctgagcttcc cgacgcaaag
    18241 cgctcgcgct ggcagaaaga gctggggatc acggagtacg atgccggcgt gctctcgggc
    18301 catccagagg tggccaagta cttcgaagaa gccacgcgtc tgttcatcga gatgagcggc
    18361 gcgaagccga gtgaagcagg gaagaaggtt ggcaacttcg ttcagtccga agttctgcgt
    18421 cacacgagca cggacgggct cgaggccttg tttccggtgg cccccaaggc aattgctgag
    18481 ctgctggcct cagtcgagga cggaacgatc aacggcaaga tcgcgaagaa ggtgtttact
    18541 tcgatggtgg agaccgggag cgcgcctaag gcgatcatcg agcagcaagg tttggctcag
    18601 gtaacggaca ccggagccat cgaggacgct gtgcgtcgga ttctcgaagc cagcccttct
    18661 caagtggcgc agtacaaaga ggggaagacg agcatcatcg gctatttcgt gggtcaggtc
    18721 atgaaggcga ccaagggcgc cgccaatcca aaagccgtca atgagattct cgggcgcctt
    18781 ctggatgagc aatgagcgct gggctttcgc gcgcaccgct cagcggcgcc gaatggtttg
    18841 cagtcgagtt tgaccccagc accgcagagc tcgtgatgct cgatcagcgc cttcttccag
    18901 gggaggtggt gttccggcgc tatcgaaagc ccgacgaagt cgctgatgca attcgcgaga
    18961 tggtcgttcg tggagcgccg gcgatcggga tcgccgcagc gtatgcgctc gcattggtcg
    19021 ctcgaaacga agccggcgat gcgaaaatgt tcttggtggc aaacggcgtg gccggccgca
    19081 ttttgaatgg gactcggccc accgcggtca atctcggctg ggcgattgcg cgcatgtccc
    19141 gcaaggcggg ggaggtctgt gagctcccgc ccgagcttcg ataccagggc atgctcgaag
    19201 ctgcagagtc cattcatcgt gaagatgtcg ccgcatgtcg ccggattgga gagctcggag
    19261 cggcagacgt gcctgaaggc gcgacgatcc tcacacactg caacgccggg gccttggcga
    19321 ccggaggctt tggaactgcg ctcggcgtga tccgggcggc ccatgcggca ggtaagaacg
    19381 tgcgggtact cgccagtgag acgcgaccct atcttcaggg cgcgcgcctc accgcttggg
    19441 agcttcatcg agatggcatc ccggtcgagg tgatcaccga caacatggcg ggccatttct
    19501 ttcagatggg tgacattcag ttcgtcgtcg tcggcagtga ccgcatcgcg gccaattccg
    19561 acatcgcgaa caaaatcgga acctactcac tggccgtgct tgcgaatcag cacggtgtgc
    19621 cgttcacggt tgcggcgccc tggagtaccg tcgacatccg aacgcgaagc ggcgctgaca
    19681 ttcccatcga ggaacgctcg cgcgaagaag tggcgcacgt ggcgggtgtc accatcgtcg
    19741 ccgacggaat cccctgcagg catcctgcgt tcgacgtcac gccggcccgg ctcatcgacg
    19801 caatttacac cgagcgcgga gtgattcgcc cgaagatcgg cgaacgcccc gacgtgttgc
    19861 gcgggtgatc gatcagagac gcaagagagc ggcgccccag ttgagacccg caccgagtgc
    19921 gaggaaacac aatagatcgc cctcgcgaat cgctccatcg c
//
LOCUS       DASRZF010000337        16692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1288351, whole genome shotgun sequence.
ACCESSION   DASRZF010000337 DASRZF010000000
VERSION     DASRZF010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 16692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16692
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1288351"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            334..816
                     /locus_tag="VFG22_14970"
     CDS             334..816
                     /locus_tag="VFG22_14970"
                     /inference="COORDINATES: protein motif:HMM:NF021435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HET6417597.1"
                     /translation="MVLTKWAGTGLVAMTLMGSLLWSPSSDAQIDPDPAAKNVLTLEI
                     SGIRETEGKIACALFWENKGFPRKHRRALRRTWVDVDGETVTCVFKRTGLGEYAASVF
                     LDRNGNGKLDTNAIGSPKEPWGVSQNASAKRFGPPLWKDAKFEYEGGPVTIPITLVYD
                     "
     gene            complement(802..1986)
                     /locus_tag="VFG22_14975"
     CDS             complement(802..1986)
                     /locus_tag="VFG22_14975"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6417598.1"
                     /translation="MRRIPVLFVPSFFGSWLTAELAMHHIVWQAIATVLFVELGALSN
                     WQGLLGMGVTLASWLGLLVLFHDGRKTRNTFDEALADLAEPNDAARLPLTQLLMPFRF
                     RRRGVTVVRDIVYREVAGKTLKLDVAMAEGSAAKRPAIMQIHGGGWVIGDKREQGWPL
                     IGHLAANGWVCFNVNYRLSPSATWPDHLVDLKYALKWIREHSDEYGVDPRFIAVTGGS
                     AGGHLTAMMALTANDPEYQPGFEEADTSLQAAMPVYGVYDFTNRLGTMQPQFRTRMLE
                     PMIMKAFFDKEPDEFHRASPIDRVHPDAPPFLIVHGDRDTLAPVEDARLFVDRLRAIS
                     HAPVMYTELKGAQHAFDIFASPRTARMLDGALRFLTAMRQRAKNATSAPAADRRVGSV
                     VD"
     gene            2117..3439
                     /gene="nhaA"
                     /locus_tag="VFG22_14980"
     CDS             2117..3439
                     /gene="nhaA"
                     /locus_tag="VFG22_14980"
                     /inference="COORDINATES: protein motif:HMM:NF018648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HET6417599.1"
                     /translation="MGKDDLAYSRNTHLPNRPIQRLQRPFQAFMHVESASGVVLLLCT
                     VIALVAANSSFAEAYDGFWHQDLRLAVGQFELVHSLAHWVNDGLMAVFFFVIGLEIKR
                     ELVIGELSDPKKVVLPVAAALGGVLVPVMIYLAFQRGQPGQGGWAVPMATDIAFVVGC
                     LSLLGKRIPYGLGILMLSLAIVDDLMAVGVIAIFYTSSISASWLVGAGVGIIAILVLN
                     RLGVRSVWIYFIAGAWVWLCTLESGVHSTIAGVIVGLLTPAHPWLGGPQFLGFLRHTD
                     DMLRKEETHVAEARDLVADLSFASREAVSPLYRIESALHPWVAFGIMPLFALANAGVP
                     IELSGIGEPVAVAVAAGLFFGKPTGIFGASWLTVKLGWATRPEGVTWPILGGASFLGG
                     IGFTMALFIASLGLSGALLVSAKIGIILGSFASAVIGMLILSSVTKKI"
     gene            3439..4080
                     /locus_tag="VFG22_14985"
     CDS             3439..4080
                     /locus_tag="VFG22_14985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417600.1"
                     /translation="MRALIVAFVLMLPAIAQAQSPPIFYSYTGSSGRTVYVNRFSMVP
                     PEKRSSARAIDLSEVSLNEELAEKLSGAVEDELRQLKDSVPCIEARQEDEAGAWWHVW
                     HRHGPWILASLAAMVLVLMTPWMVARTPPGVWSRLLMVALPALAMTALVAVSATRASS
                     SLEAVHELSKLCDANEKEASPQKQVVRLNEMRGYIEKLYKDRFDRIDAIIEIE"
     gene            4077..4289
                     /locus_tag="VFG22_14990"
     CDS             4077..4289
                     /locus_tag="VFG22_14990"
                     /inference="COORDINATES: protein motif:HMM:NF025733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich CWC family protein"
                     /protein_id="HET6417601.1"
                     /translation="MTEPSKSNETGRCPLCGEPNECAIVAGRDPESCWCMSATMSPRA
                     LAAIPDELQGKVCICARCASGASAVD"
     gene            complement(4317..5228)
                     /locus_tag="VFG22_14995"
     CDS             complement(4317..5228)
                     /locus_tag="VFG22_14995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET6417602.1"
                     /translation="MGHQHHHHHEASVADAAGNRRRLAMALVLTAAYMVAEIVGGLAA
                     NSLALLADAGHMFSDAAALGLSLVAVILAQRPATSQRTYGFHRAEILAALLNGAALLT
                     LSVLIAREAWERLSAPPEVEGGLMLLIAVGGLAINLANLRILSGGRQSSLNVRGAWLH
                     VMADTLGSVGAISAGAAIYFLGWRWADPVASFIIASLVLYSAWGLIRETLDVLMEGVP
                     KGISIEEVTAALTELGGVMGTHDLHVWSLTSGRNIATAHLIITEDADHQGIIDAANRM
                     LASRFSIHHATIQVEHDQLASQCHPCG"
     gene            5365..6018
                     /locus_tag="VFG22_15000"
     CDS             5365..6018
                     /locus_tag="VFG22_15000"
                     /inference="COORDINATES: protein motif:HMM:NF018595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HET6417603.1"
                     /translation="MSDKKLATYDGEEADVQWDGRLCIHVGECGRANNNLFVGGRQPW
                     CQPDRVSVEEVADVVARCPSGALTYTRKDGGPEEVAETENVVSVMYNGPLYLRGELEV
                     DGAADDMTGVRFRAALCRCGQSKSKPFCDNSHEAAGFRDYGAVGESGQGFEAPGGPLK
                     VGRAPNGPLLLNGNFSIVAASGRTAWKGSKAALCRCGQSKNKPFCDGAHKDAGFKAE"
     gene            6048..7676
                     /locus_tag="VFG22_15005"
     CDS             6048..7676
                     /locus_tag="VFG22_15005"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HET6417604.1"
                     /translation="MSFSTMMDRPLLISDLFEHGRRLYADSRVITAGAQNDRISSYAE
                     VADRTEQLAKALAKLGVQREDRVGTFCWNNQEHLEAYFAISSMGAVMHTLNIRLFPEQ
                     LTFVINHARDKVIIVDDSLVPLLGKVAKQMATVEHIIVVGDGDDSPLREAPRATLHRY
                     DALISSESPGIDWPLLDERAPASMAYTSGTTGDPKGVVYSHRSTVLHSYGITSGAVTG
                     LSEGDMVLTIVPMFHANCWGLPYAGWWVGTDFLQPERFLQPEPLVAMIKRHRPTFAGA
                     VPSIWTGVLHYGEREDIDLSSFRLVICGGSAVPKSLMKAFEERYGVTILQLWGMTETS
                     PLGSIASVPPGVEGEEAWTYRVRTGRPVAGIELRIVDEQGAALPWDGKSVGEIEARGP
                     WVTGSYYGVDAPEKFHDGWLRTGDVGFVDARGYMQITDRSKDVIKSGGEWISSVELEN
                     EIIAHPDVAEAAVVGIPDEKWDERPLACVVRKDGSNISPNELRAYLENKVAKWWLPES
                     WAFIEEIPKTSVGKFDKKVLRARHAAGDLTVVST"
     gene            complement(7696..8130)
                     /locus_tag="VFG22_15010"
     CDS             complement(7696..8130)
                     /locus_tag="VFG22_15010"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET6417605.1"
                     /translation="MSSVVEDVIAFYRMFASFEREEICCGTVSPAQCVLLQTLTEGEW
                     DVSSLAFQARVTKSAMTRLIDGLETRDWVEREKAEDDGRRVLVKLTSDGKKEAKRLRG
                     LTEQSINTILETIPRAERPQVIRSIHLLRDAAERTRTRLDCC"
     gene            complement(8127..8939)
                     /gene="arsM"
                     /locus_tag="VFG22_15015"
     CDS             complement(8127..8939)
                     /gene="arsM"
                     /locus_tag="VFG22_15015"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HET6417606.1"
                     /translation="MDKNRIHEVVRSGYAEVARQGGGCCGNTAAKAHGQTAALIGYTE
                     ADISGTAAEGNLGLGCGNPTALASLKPGETVVDLGSGAGFDALLSAEKLGPEGRFIGV
                     DMTPEMLERARINAVNAGFARTVEFREGLIENLPVASESVDVVISNCVINLSPDKAPV
                     FREAYRVLKPGGRLAVSDIVLTEPLPTDIARFAASWIACVGGALTEEEYFGHIRAAGF
                     ENIEHNRKPAAPMLTSSLQDPMWKAAAELVGEERIQELSNTVFSYSITAVKR"
     gene            complement(8996..9868)
                     /locus_tag="VFG22_15020"
     CDS             complement(8996..9868)
                     /locus_tag="VFG22_15020"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6417607.1"
                     /translation="MLADSSFENVTRGGQTWRYSDSGRGHPVVLIHGFPDTPHSYEGI
                     ARALNAAGYRTILPYLRGYHPDTLVPERPYDALHLAEDAIGLLDALGLESAVLIGHDW
                     GATLVYGAAALAPERVDAIVPIAIPHPLTLKPKHALQAIGLLVMARHFLYFQLPWAEA
                     GTRRGDLAYIEALYQRWAPNWTGPERDAALARVKEAFANPKVLSAAIDYYRAVGTKID
                     RRVLAPIACRGLMVAGGKDFGGHLGPYKKSLALFGGGAELMVIPEAGHWPHRENEPLF
                     TRNLVNFLGREAAP"
     gene            9996..10727
                     /locus_tag="VFG22_15025"
     CDS             9996..10727
                     /locus_tag="VFG22_15025"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6417608.1"
                     /translation="MPEPTPSSERAAILVAEDVSKVYQMGDVQVHALRGVDVTLYEGE
                     LMVLLGPSGSGKSTLLNILGALDVPSSGRVLYRDADIAGHGERELTRFRRDHVGFVFQ
                     FYNLVPSLTAKENVALVTEIAKTPLDPREALELVGLTERMDHFPAQLSGGEQQRVAIA
                     RAVAKQPEILLCDEPTGALDFKTGIRVLEVLDEVNRSLGTTTAVITHHAPIASMADRV
                     VSLSDGIVASDHTNQAKVSPAELTW"
     gene            10721..13090
                     /locus_tag="VFG22_15030"
     CDS             10721..13090
                     /locus_tag="VFG22_15030"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6417609.1"
                     /translation="MVKVLDRKLLRDLTRLKSQAFTIALVVAVGVANYITLKSAWRAL
                     GDSRAAYYEEYRFADIFARLKRAPDSVADRIQDLKGVASVYPRVVQDVLLPLEDMITP
                     AHGQIISLPMHGRPQMNDVYVKAGRFPEPGRANEVLVYEPFALEHGLLPGSTVAAILN
                     GKLHELIVVGLALSPEYVFAVEPGGITSDDERFAPMWMSKSAVAAAYQMEGAFNDLVL
                     RLEPGANERAVLVAVDRMLRPYGGLGAYGRDLQVSNSILSGELAQIEAMVIFLPGVFL
                     AVAAFLLNVVFSRLVTLQRGQIAVIKAVGYTNLAIALHYLKMVFVIVLIGSLLGVGLG
                     AWLADAMLDLYAQFFRLPMARGRIDLPVAINAIIASLVAALLGIGLAVRNAVTLPPAE
                     AMRPPSPARYRASPIERFPGLRSIHQTTMMVFREIWRQPLRTVLSSTGIAFALAIIIL
                     GRFGKDAHGPILDLQFGRQQREDISVNFTEEVDQRALGYLGHLPGVDQVDGFRTTPVR
                     FRSGPRWRDSVLIGLPPDSHLRRLFNRQFRPARIPHTGVLITDVLADILGAKPGDFIE
                     VELKEGDHGTRRVRITGTIDEAFGIQGYMQKSRLHELLRENPTINTALLRVDPARESE
                     LQTRLADIPTVLSIHRKRTLLEKFEEQTSRTMRVITLILTLFATAIAVAVVYNNARVS
                     LSVRSRDLATLRVLGFTRAEISAILLGEQAVQVFLGIPFGLVLGTWGAKALLELQADP
                     EQFRMPMLISSQTYAFSVVVILAAAVLSALLVRRRLDTLDLIGVLKTRE"
     gene            13104..14309
                     /locus_tag="VFG22_15035"
     CDS             13104..14309
                     /locus_tag="VFG22_15035"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET6417610.1"
                     /translation="MDKASGLRVWIRRALIALALIAVVGLIVVAWIPNPVEVEVAKVS
                     RGRLVVTVNEDGRTRVKDRYTVSAPLTGNLTRVTLDPGDSIEEGQVLLRLVPLPPPLL
                     DSRSRAEAKARVDGAMAARRQAQAAVNRARFQRDFAKQESERALAVVQQGGLARSDAD
                     RAVSTYRSSEEELHSAEFGARVAEHELTLAQSALMRLSGKGEGEELDIISPVAGQVLK
                     IYQESEGVVQGGTPILEVGDPAALEVVVDVLSQDATRIPIGASTTIERWGGKVPLRSH
                     VRVVEPSAFTKLSALGVEEQRVNVIIDMDEDHERWSSLGDGYRVEARITVWEGKDVLK
                     VPASAVFRSDEAWATFVVDEGAAIIRTVELGETNGLETEVLSGLDEGESVIAYPSDSV
                     RDGVSVKAR"
     gene            14317..15180
                     /locus_tag="VFG22_15040"
     CDS             14317..15180
                     /locus_tag="VFG22_15040"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HET6417611.1"
                     /translation="MNPIIDSTTLTEVFQDRQIMSHLVATLVLLVSVLSIRYVALRFV
                     RRALPSKDKLRLRWSSQIRGLSYAILAFGLLTIWAQELQTLAVSFVVLAMAIVWATKE
                     TIACVQGAVYRVSSNAFEVGDRIAVGSVRGDVIDAGLLSTLVLEVGQGHQRTGRTISI
                     PNSLLMTEPVLNESLAGEYMLHLMSIPVDRNADLAAIERKALSAARDACAEFIDDVRR
                     PIAARYRRHGLVPPIVDPRVTYQVVDKNTVNIMLRIPTPSRLERQVEQRVLRAVLGVP
                     KDRDTLPPPSR"
     gene            15177..15680
                     /locus_tag="VFG22_15045"
     CDS             15177..15680
                     /locus_tag="VFG22_15045"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET6417612.1"
                     /translation="MNSDDLEIRIARLEAIEAIKALKARYWHCCDTKDVEGVRDCFLD
                     GPVEIHYDGPVGLVNHRDGLYQVFKDVACQTQIVEMHHGGPPNIEVQGPDRASGRWGL
                     VYHLMNTDLRTVSVVGGYYDDEYTRVDGKWWISKARFEVCSAVTYGWKDAGLRMLHAA
                     AFLPEAK"
     gene            15727..>16692
                     /locus_tag="VFG22_15050"
     CDS             15727..>16692
                     /locus_tag="VFG22_15050"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET6417613.1"
                     /translation="MEEIRPVDGLRCLVTGSAGFVGSNLVRALLERGCEVHGFDRAPA
                     TFEDPKLRWFRGDIRNRDALSEAMKGVDTVFHTAAMIETLTYTPRPFAEQVRSVNIEG
                     TRSVLQTARDSGVRRLVHTSSIITVFGDGHSGVDETAPYSTASDLYSSTKVASEQLVL
                     AANGESGMFTCALRPGGIYGPGERNTLIGPMIKALKQGVPIVVFGDGATRMDYTYIDN
                     LVDAQIRAAERLVEGSPVCGEAYFVTDGDPINTGDFSQTLVGQMGLDARTLRVPGRLA
                     RAIATAGERVFQLLGKPKPPVSIVDVELCVHDSYFSIDSARRDLGY"
BASE COUNT         3101 a         5127 c         5261 g         3203 t
ORIGIN      
        1 cgaaaccctg ctgctccggg cttgccgtcg gccattcccc aaccggcgct gtggtgtccg
       61 ccgcacaact cgacaggccg agagaaagga tcacgcatat gcgagccgag cacagaaatg
      121 cctggattct tggcatggga agcagctcca acgttaggcc gacgtgcggc agactacctg
      181 cgttcaccgg accgatccat gcgacgaatc agcactcgcg agcgcaagca tttggcaaaa
      241 atgggcgtct cgtctaaaga tttggcacgg ccgcgcgtca gttctagcat gacggtggtc
      301 ggcgagcagg gcagggaacg cggggagcat tggatggttt tgactaaatg ggcgggcacg
      361 ggcttggtcg cgatgacgct catgggctcc ctcctatggt ctccatcttc ggacgcccag
      421 atcgaccctg accctgcggc caagaacgtc ctcacgttgg agatcagcgg cattcgcgag
      481 accgaaggca agatcgcctg tgctctcttc tgggaaaaca agggctttcc gcgaaagcat
      541 cggcgtgcgc tgcggcgcac ttgggtcgac gtcgacggag aaacggtcac atgcgtattc
      601 aagcgtacgg ggctcggcga gtacgcggca tcggtgtttc tcgataggaa cggcaacgga
      661 aagctagaca ccaacgccat cgggagccca aaggagccct ggggtgtctc gcagaatgct
      721 tccgccaaga ggtttggccc gccgctctgg aaggacgcca agttcgagta cgaaggcgga
      781 ccggtaacga ttccgatcac gctagtctac gactgacccg acgcggcgat cggccgctgg
      841 agccgaggtc gcattttttg cgcgttggcg catcgccgtc aggaaacgca gagctccatc
      901 gagcattcga gctgttctcg gcgacgcaaa aatgtcgaac gcgtgctgtg ctcccttgag
      961 ctccgtgtac atcactggcg cgtgtgaaat cgctcgaagc cggtcgacga acagccgagc
     1021 atcctccact ggtgccaggg tatcccggtc tccatgaacg atgaggaagg gaggtgcgtc
     1081 cgggtgaacc cgatcgatgg gggaagcgcg atgaaattcg tcaggctcct tgtcgaagaa
     1141 agccttcatg atcatcggct ctagcatgcg ggtccgaaat tgcggctgca tggtgccgag
     1201 ccggttggtg aaatcataga ctccgtacac tggcatggcc gcttgtaagc tcgtgtctgc
     1261 ttcttcgaag cccggttgat actccgggtc gttcgcggtc aaggccatca tggccgtgag
     1321 gtgtccgccc gcggagcctc cggtcacggc gatgaagcgc gggtcgacgc cgtattcgtc
     1381 cgagtgctcg cgaatccact tcagtgcgta tttcaaatca acgaggtgat cgggccaggt
     1441 tgcagaaggg ctcagccgat agttgacgtt gaagcagacc caaccgttcg ctgcgaggtg
     1501 cccaatcagc ggccacccct gttcccgttt gtcgccgatg acccacccgc ctccgtggat
     1561 ctgcatgatc gccggccgtt tggccgccga accctccgcc atggcgacgt ccaatttcag
     1621 tgttttgcca gcgacctcgc ggtagacgat gtcgcgaacg accgtcaccc cgcggcgccg
     1681 aaatcggaaa ggcatcagca gctgcgtcag cggcagccgc gctgcatcgt tcggttcggc
     1741 gagatccgcc agcgcttcat cgaacgtgtt gcgtgtcttc cgaccatcat ggaacaagac
     1801 gagaaggccg agccaggaag ccagcgtgac gcccatcccg agtaggcctt gccagttcga
     1861 gagcgcccca agctcgacga aaagcaccgt cgcgatggcc tgccaaacga tgtggtgcat
     1921 cgccagctct gcggttagcc acgagccgaa aaaactaggc acgaagagca ctggaattcg
     1981 gcgcacgggt aagaacgcat tcaccgtgaa caccgcgccg accagactga ccccgagaaa
     2041 cagtcacccg acggtggctt cgctgatcca cataggtcct ggccttctag cacgttccgc
     2101 gataagccgg tcgccgatgg gcaaggacga cctcgcgtat tctcgcaaca cccacctgcc
     2161 gaatcgtccg attcagcgcc ttcagcgacc gttccaggcc ttcatgcacg tcgaatcggc
     2221 gagcggcgtc gttctcttac tctgcacggt catcgcgttg gttgctgcca attcgtcgtt
     2281 tgcggaggct tatgacggct tctggcatca ggacctgcgc ctcgccgtgg gccagttcga
     2341 gctcgttcat tcgctcgctc actgggtcaa cgacgggctc atggcggtat tcttcttcgt
     2401 gatcggtctc gagatcaaac gcgagctcgt aatcggagag ctcagcgatc cgaagaaggt
     2461 cgtgctgccg gtcgcagcgg cgctaggtgg agtgctcgtg ccggtcatga tctatctcgc
     2521 gtttcagcgc gggcaaccgg gccaaggcgg gtgggccgtt ccgatggcaa ccgacatcgc
     2581 attcgtggtc gggtgcttgt ccctgctcgg caaacgcatc ccctatggac tcggcattct
     2641 gatgctttcg ctcgcaatcg tggatgacct catggcagtc ggcgtgattg caatcttcta
     2701 tacctcgtcg atcagcgcga gctggttggt cggcgcaggg gtcgggatca tcgcgatcct
     2761 cgtcctgaac cgcctcggcg tacgctcggt ttggatctat ttcatcgcag gtgcttgggt
     2821 ctggctctgc accctcgaat cgggcgtgca ctcgacgatc gctggtgtga tcgtcggctt
     2881 gctcacgccc gcgcacccat ggctaggagg gccacagttc ctcggcttct tgcgccacac
     2941 cgacgacatg cttcgcaagg aagagaccca cgttgcggag gcgagggatc tggtcgccga
     3001 cctctcgttt gcatcccgcg aggccgtgtc gcccctgtac cgcatcgaaa gcgccctcca
     3061 tccctgggtc gcgtttggca tcatgccgct cttcgcgctg gccaatgccg gtgtccccat
     3121 cgagctgagc ggaatcggag agccggttgc cgtggcagtc gcagccgggc tgttctttgg
     3181 caagccgacc ggcatctttg gcgcatcctg gctgacggtg aaactcggat gggctacgcg
     3241 gcccgagggc gtcacctggc ctattctcgg aggcgcttcc tttctcgggg gcatcggatt
     3301 caccatggcg ctgttcattg cctccctggg cctctccggg gcactcttgg tgtccgcgaa
     3361 gatcggcatc atcctaggct cgttcgccag tgccgtcatc ggcatgctga tcttgtcctc
     3421 ggttaccaag aagatctgat gcgagctctc atcgtcgctt tcgtcctcat gcttccggcc
     3481 attgcccaag ctcaatcccc accgattttc tattcctaca cggggagctc cggccgcacc
     3541 gtctatgtga accgattctc gatggtgccg ccggagaaac gttcctcagc gcgcgcgatc
     3601 gacttgtccg aggtcagtct caacgaggag ctcgccgaaa agctatccgg cgccgtcgaa
     3661 gacgagcttc gccaattgaa ggactcggtt ccctgtatcg aagcccgcca agaagacgag
     3721 gcaggggctt ggtggcacgt ctggcaccgc catgggccgt ggattttggc tagcctcgct
     3781 gcgatggttc tcgtgctgat gacgccctgg atggttgcgc gcacgccacc tggtgtctgg
     3841 tcacgcttgc tgatggtggc gctgcctgcc ctcgcgatga cggccttggt cgccgtgtcg
     3901 gcgacgcggg caagcagctc cctcgaagcg gttcatgagc tcagcaagct ctgtgacgca
     3961 aacgagaagg aggcgtcgcc tcagaaacag gtcgtgaggt tgaacgagat gcgcggctac
     4021 atcgagaagc tctacaagga ccggttcgac agaatcgacg ccattatcga gatcgaatga
     4081 ctgaacccag caaatcgaac gagaccggca ggtgccctct ctgtggcgag cccaacgagt
     4141 gcgccatcgt cgcaggacgg gacccggagt cctgctggtg tatgagcgcg acgatgagcc
     4201 ctagggcgct cgcagcgatt ccggacgaat tacaaggcaa ggtctgcatc tgcgcgcgct
     4261 gtgccagcgg cgcatccgcc gtcgactgag cagagcgggc aacgaggcgg gccccgtcag
     4321 ccgcaggggt gacattggct tgcgagctga tcgtgctcga cttgaatcgt cgcgtgatgg
     4381 atcgaaaagc ggctcgctag catccggttc gccgcgtcga tgatgccttg gtgatcggcg
     4441 tcctcggtga tgatcagatg ggccgttgcg atgttacgcc cactggtcag cgaccaaacg
     4501 tgcaggtcgt gcgtacccat cacgccccca agctcggtga gcgccgccgt gacctcttcg
     4561 attgaaatgc ctttcgggac accttccatc agtacgtcga gcgtttcccg tatgagcccc
     4621 caggcggagt acaagaccag cgacgcgatg atgaaggacg cgaccgggtc ggcccagcgc
     4681 cagcccagaa aatagatggc agcacccgct gaaatcgcgc ccacgctgcc gagagtgtca
     4741 gccatgacgt gcagccatgc accgcgcacg ttgagactgc tttggcgacc tccgctgagg
     4801 attcgcaggt tcgccaagtt gatggccaag ccgccaacgg cgatcaagag catcaacccg
     4861 ccctccacct ccggtggcgc ggagagtcgc tcccaggcct cgcgcgcgat gagaacggag
     4921 agggtgagca gtgcggcgcc attgaggagt gccgccagaa tttccgcgcg gtggaatccg
     4981 taggttcgct gcgaggtcgc aggacgctgc gcgagaatca ctgccaccag agagagtccg
     5041 agcgccgcgg catcggagaa catgtgtccc gcatcggcca gcaacgcgag agaattggcg
     5101 gcgagcccgc cgacgatctc ggcgaccatg tatgccgcgg tcagtaccag ggccatggcg
     5161 agccgccggc ggttcccggc ggcatccgcg acgcttgcct catggtggtg gtggtgctgg
     5221 tgtcccatct cggcttcctg cttaccacgc cgccacgcgc cgggcgattc agcgtcagtt
     5281 cgctgtggtc gtttgctcgt ggctgtagta gaaaatcgga ctcggggtgg tgcccatcga
     5341 agctgaaacg tgaggagaaa ccccatgagt gacaaaaagc tagcaactta cgatggcgaa
     5401 gaggcagatg tgcaatggga cgggcgtctc tgcattcacg tcggcgagtg tggtcgcgcc
     5461 aacaacaacc tgttcgtcgg cggccggcag ccctggtgcc agccggaccg cgtgagcgtc
     5521 gaggaggtcg ccgacgtggt ggctcgctgc ccgagcggag cactgaccta cacgcgcaaa
     5581 gacgggggcc cggaggaggt cgctgagacc gagaacgtgg tttcggtgat gtacaacggg
     5641 ccgctctatc ttcgtggtga gctcgaggtg gacggagcgg cggacgacat gaccggggtg
     5701 cgttttcggg cggcgctctg ccgttgcggg cagtccaaga gcaagccgtt ttgcgacaac
     5761 agccacgaag cagcgggctt cagggactac ggagccgtgg gtgagagtgg gcaggggttc
     5821 gaggctcccg gtgggccctt gaaggtcggc cgcgcaccga acgggccgct ccttctcaat
     5881 gggaacttca gcatcgtcgc ggccagcggc cgtacggcat ggaagggcag caaagccgca
     5941 ctgtgccgat gcgggcaatc caagaacaag ccgttctgcg acggagctca caaggacgcc
     6001 ggtttcaagg ccgagtaagc agcgcacggt cgctacggga ggtttaagtg agcttttcga
     6061 cgatgatgga tcggccgctt ctgatatcgg acctgttcga gcatgggcgg cgcttgtacg
     6121 ccgatagccg ggtgatcacc gcaggcgctc agaacgatcg aatcagcagc tacgccgagg
     6181 ttgcggatcg gaccgagcaa cttgccaagg cgctcgcgaa gctcggcgtg cagcgcgagg
     6241 atcgcgtcgg cacgttctgc tggaacaacc aagagcactt ggaggcatac ttcgcaattt
     6301 cctcgatggg cgccgtcatg cacacgctca acatcaggtt gtttcccgaa caactcacgt
     6361 tcgtgatcaa ccatgcccgc gacaaggtga tcatcgtcga cgacagcctc gtgccattgc
     6421 tcggaaaagt cgcgaagcag atggcgacgg tggagcacat catcgtggtg ggggatggcg
     6481 acgactcgcc gctgcgcgaa gctccgcgcg ccacccttca tcgatacgat gcgctcatct
     6541 cttcggagtc gcctgggatc gattggcccc tgctcgacga gcgggcgccc gcatccatgg
     6601 catacacgag cggcacgacc ggcgatccaa agggcgtcgt ctacagccat cgctctacgg
     6661 tgctgcattc gtatggcatc acctccggag ccgtcaccgg gctgagcgag ggggacatgg
     6721 tgctcaccat cgtgccgatg tttcacgcga actgctgggg gctgccgtac gccggctggt
     6781 gggttggtac agacttcctg caaccggaac gattcctgca gcccgaaccc ttggtcgcga
     6841 tgatcaaacg gcatcggccg acattcgcgg gggcggtccc gagcatctgg actggggtgc
     6901 tccactacgg cgagcgcgag gacatcgact tgtcctcgtt tcgactggtg atctgcggcg
     6961 gctctgccgt tcccaagtcc ttgatgaagg ccttcgaaga gcgctatggg gtcacgatcc
     7021 tacagctctg gggaatgacg gagacgagcc cgctcggctc gatcgcatcg gtgcctcctg
     7081 gcgtcgaagg ggaagaagcc tggacgtatc gggtccgaac cgggcgaccc gtcgccggga
     7141 tcgagctccg catcgtcgat gagcagggcg cagcgctccc ctgggacggc aagtccgtgg
     7201 gcgaaatcga agcgcgcggc ccctgggtga ccgggagcta ctatggcgtc gacgcgccgg
     7261 agaagtttca cgacggttgg ctgcggaccg gcgacgttgg attcgtggat gcgcgcggct
     7321 acatgcagat caccgaccgc tcgaaggacg tgatcaagtc cggcggcgaa tggatttcct
     7381 cggtcgagct cgagaacgaa atcatcgcgc accccgacgt tgccgaagcg gccgtcgtag
     7441 gcatccctga cgagaaatgg gatgagcgcc ccctcgcttg tgtcgttcgt aaagacggca
     7501 gtaacatcag cccaaacgag ctccgcgcct acctcgaaaa caaggtcgcg aagtggtggc
     7561 tccccgagag ctgggccttc atcgaggaga tcccgaagac cagcgtgggt aagttcgaca
     7621 agaaggtgct gagagcacga catgctgccg gcgatctaac ggtagtcagc acctgagcgg
     7681 ggcgtcacct gcttcctagc agcagtcgag ccgggtgcgt gttcgttcgg cagcatctcg
     7741 gagcaggtgg atactgcgaa tcacttgcgg ccgctccgct ctcgggatcg tctcgagaat
     7801 cgtattgatg ctctgctccg tcaggccgcg aagtcgcttg gcttccttct ttccgtcgga
     7861 cgtgagcttc accagcacgc gccgaccgtc gtcttccgcc ttctcgcgct cgacccaatc
     7921 gcgggtttcg aggccgtcga tgaggcgcgt catcgcgctc ttggtgactc tggcctgaaa
     7981 ggccaggctg ctcacatccc attcgccctc ggtcaaggtt tgaaggagca cgcattgggc
     8041 cgggctcacg gtcccgcagc agatctcttc gcgctcgaac gaggcgaaca tgcggtagaa
     8101 ggcgatgacg tcttcgacga ccgagctcat cgtttcacgg cggtgatcga gtagctgaag
     8161 accgtgttgg agagctcctg aattcgctct tctcccacga gctccgcggc cgccttccac
     8221 attggatcct ggaggctcga cgtcagcatt ggggctgccg gtttacggtt gtgctcgatg
     8281 ttctcgaagc ccgcggcacg gatgtgccca aagtactcct cctcggtcag ggcgccgccc
     8341 acgcatgcga tccaacttgc agcgaatctg gcgatgtcgg tgggcaaggg ctcggtgagg
     8401 acgatgtcac tgacggcgag gcgcccgcca ggcttgagca cccgatacgc ttcgcggaag
     8461 acgggcgcct tgtcggggct gaggttgatc acacagttgg agatcaccac gtccacgctc
     8521 tccgatgcga ccggcaggtt ttcgatcaga ccttcgcgaa actcgacggt gcgcgcgaac
     8581 cccgcgttca ccgcattgat ccgcgcccgt tcgagcattt ccggcgtcat gtccacgccg
     8641 atgaatcggc cttcggggcc cagcttttcg gcggagagca aggcatcgaa gcctgctccg
     8701 gagcccaggt ccaccacggt ttcgcccggc ttcaggctgg ccaaggccgt cgggttgccg
     8761 cagccaagcc cgaggttgcc ttccgccgcc gttccgctga tgtccgcttc ggtgtagccg
     8821 atcagggcgg cggtctggcc gtgggccttc gcggctgtgt tgccgcagca gccgccacct
     8881 tgccgcgcaa cctcggcata tcccgacctc accacctcat gtatgcgatt cttatccatc
     8941 tgagcctccg atagttgcat gacacactca attagttgca tcgctcaacc atttgtcaag
     9001 gcgcggcttc tcgcccgaga aaatttacca gatttcttgt aaataggggc tcgttttctc
     9061 ggtgaggcca gtgcccggct tccgggatca ccatgagctc ggcgccgccg ccgaagaggg
     9121 ctagcgattt cttgtacggg ccgaggtggc cgccgaagtc cttgcctccc gcgaccatca
     9181 gaccgcggca tgcaatcggc gccagcacgc ggcggtcgat cttcgtgcct actgcgcggt
     9241 agtagtcgat cgctgcgctc agcaccttcg ggttcgcgaa agcttccttc acgcgggcta
     9301 gcgcggcatc tcgctcaggg ccggtccagt tgggtgccca gcgctgatag agcgcctcga
     9361 tgtaggcgag atcgccgcgc cgcgtcccgg cttcagccca aggcaactgg aaatacagga
     9421 agtgcctcgc catcacgagc aggccgatcg cttggagcgc gtgcttcggc ttcaacgtga
     9481 gcgggtgtgg aatggcgatg ggaacaatcg cgtctacacg ttcaggagcc agagccgcgg
     9541 caccatacac cagcgtcgca ccccaatcgt ggcctatgag gaccgcggat tccaagccca
     9601 gcgcgtcgag gagcccgatc gcgtcctcgg cgaggtgcag cgcatcgtac ggtcgttcag
     9661 gcacgagagt gtccgggtgg tagccgcgca agtagggcaa gatcgttcgg tagcccgctg
     9721 cgttgagagc acgcgcaatc ccctcatacg aatgtggggt gtcgggaaac ccgtgaatga
     9781 gcaccaccgg atgccctcga cccgagtcgg agtatcgcca agtctgcccg ccgcgcgtga
     9841 cgttctcgaa gcttgaatcg gcgagcatgc gaatctccgt cggtgatgaa tgcgtccctg
     9901 cgatggtgat agcctccaag tccagatcca ttcaaggatg agttgacccg cttcgacaca
     9961 aagcgtacga acgtgcacgc aactgggacg acgccatgcc agagcccacc ccgagcagcg
    10021 aacgtgccgc cattctcgtc gccgaggatg tgagcaaggt gtaccagatg ggcgacgtgc
    10081 aggtgcacgc gctgaggggt gtggacgtga ccctctacga gggcgagctc atggttttgc
    10141 tcggcccctc aggaagtgga aaatcgactc tgctcaacat actcggcgcc ctcgacgtgc
    10201 cgagcagcgg gcgagtgctc taccgagacg ctgacatcgc aggccatggc gagcgagagc
    10261 tcacccggtt ccgtcgcgat cacgtaggct tcgtgttcca attctacaat ctcgtgccga
    10321 gcctcacggc caaggagaac gtcgcactcg tcaccgagat cgccaagacc cccttggatc
    10381 cccgcgaagc gctggagctc gtcggcctga ccgaacggat ggatcacttt ccggcgcagc
    10441 tctccggtgg cgaacagcag cgcgttgcca tcgcgcgcgc agtggccaag cagcccgaga
    10501 ttctcctttg cgacgagccc acgggtgcac tggacttcaa gacgggcatt cgggtgctcg
    10561 aggtcctcga cgaggtcaac cgctcgctcg gaacgacgac ggccgtgatt acccaccatg
    10621 cacccatcgc gtcgatggcg gatcgtgtcg tttcgctctc cgatgggatt gtcgccagcg
    10681 atcataccaa ccaagcaaag gtgtccccag ccgaactcac atggtgaagg tcctcgaccg
    10741 aaagctcctc cgcgacctca cgcgcctcaa gagccaggct ttcacgatcg ctctggtcgt
    10801 tgcggtcggc gtggccaact acatcacctt gaaaagcgcg tggcgcgcgc tcggcgactc
    10861 gcgagccgca tactacgagg aataccgctt cgctgacatc ttcgcgcgtc taaagcgggc
    10921 gcccgactcc gttgcggatc gaattcagga cctcaagggc gtcgcgtccg tctacccgcg
    10981 agtcgtgcag gacgttctgc ttcccttgga ggacatgatc acgcccgccc atggtcaaat
    11041 catctcgctg cccatgcacg gccggccgca gatgaacgac gtgtacgtca aagccggtcg
    11101 ttttcccgag cccggacggg caaacgaggt cctcgtctac gagccgtttg ccctcgagca
    11161 tggccttctg ccgggatcga ccgttgctgc cattctcaat gggaagctcc acgagctgat
    11221 cgtcgtcggt ctcgcgctgt ccccggagta cgtcttcgcg gtggagcccg ggggcatcac
    11281 ctccgacgac gagcggttcg ccccgatgtg gatgtcgaaa agcgccgtcg cggctgccta
    11341 tcaaatggag ggcgctttca acgatctcgt tctccgcctg gaacctggcg cgaacgaacg
    11401 cgcggtgctg gtcgcggtcg atcgcatgct gaggccgtac ggtgggctgg gcgcctacgg
    11461 tcgagatctt caggtctcca actcgatcct cagtggagag ctcgcccaga tcgaggccat
    11521 ggtgattttc ctccccggcg tcttcctggc ggtcgccgca tttctcctga acgtcgtgtt
    11581 ctccaggctg gtgacgcttc aacgcggcca gatcgcggtg atcaaagccg ttggctacac
    11641 caacctggcc attgcgctgc actacttgaa gatggtcttc gtcatcgtcc tgatcggctc
    11701 attgctcggg gttgggctgg gggcctggct ggctgacgcg atgctcgacc tctacgccca
    11761 gttcttccgc ctgccgatgg cgcgcgggcg catcgatctc ccagtggcga tcaacgcgat
    11821 catcgcgagc ctcgtggccg cgctgctggg gataggcctc gcggtacgaa acgccgtgac
    11881 ccttccacca gccgaagcga tgcgtccccc ttctcccgcc cgctaccggg cctcgccgat
    11941 cgagcgattt ccggggctcc gctcgattca ccagaccacc atgatggtct ttcgggagat
    12001 ctggcggcag cccttgcgca cggttctctc gtccaccgga atcgcattcg cgctggcgat
    12061 catcattttg ggtaggtttg gcaaagacgc gcatgggccg attctcgacc ttcaattcgg
    12121 tcgacagcaa cgcgaagaca tcagcgtgaa ttttaccgaa gaggtcgatc agcgtgcgct
    12181 cggatatctc gggcatcttc ctggcgtcga tcaggtcgat gggtttcgaa ctactcccgt
    12241 gcggtttcgc tcgggaccgc gttggcgaga cagcgtactg attgggctcc cgccagacag
    12301 ccacttgagg cggctattca accggcagtt taggcctgcg cgcattccac acaccggtgt
    12361 cctgatcacc gacgtcttgg cagacattct cggcgcgaaa cccggtgact tcatcgaagt
    12421 cgagctcaaa gaaggcgatc acggaacgag gcgggttcgc atcacaggca cgatcgacga
    12481 ggcgtttggc atccaaggct acatgcagaa gagccgactt cacgagctct tgagagagaa
    12541 tccgacgatc aatacggcgc tgctccgcgt cgaccctgca cgtgaatccg agctccagac
    12601 acgccttgcc gacattccga cggtactgag catccatcga aagcgaacgt tgctcgagaa
    12661 attcgaagag cagacgagtc gcaccatgcg ggtgattacg ctcattctca ctctctttgc
    12721 gaccgcgatt gccgtcgcgg tcgtgtacaa caatgctcgc gtgagcctct ccgtacgcag
    12781 ccgggacctc gccaccctgc gagtgcttgg attcactcgt gccgagatct cggcgatctt
    12841 gctcggcgag caggcggtcc aggtgttctt ggggataccg ttcgggctcg tgctcggaac
    12901 ctggggcgca aaggccttgc tggagcttca ggcggacccg gagcaattcc ggatgccgat
    12961 gctgatatcg tcccaaacct atgccttctc cgtcgtggta atcttggccg cggccgtgtt
    13021 gagcgcgctg ctcgtccggc ggaggctcga cactttggat ctaattggcg tgctcaagac
    13081 gagggagtaa agagggatgg cccatggata aggcgtccgg tttgcgagtt tggattcgaa
    13141 gggctctgat cgctctggcc ctgatcgccg tagtggggct gatcgtcgtt gcctggattc
    13201 ccaaccccgt cgaggtcgaa gtcgcgaagg tctctcgcgg tcgcttggtg gtgacggtga
    13261 acgaggacgg cagaacccgg gtcaaggacc gttatacggt gtccgctcca ctcactggca
    13321 acctcacgcg ggtcacgctc gaccccggag actcgatcga agagggacag gtcctcctgc
    13381 gactggtacc gttgccgccc ccgctgctcg attctcgttc gagggccgag gcaaaggctc
    13441 gcgtggatgg agcaatggcc gcgcggcgtc aggcccaggc agccgtcaat cgcgcgaggt
    13501 ttcagcgtga tttcgccaag caggagtccg agcgcgccct tgctgtcgtg caacaaggcg
    13561 gcctggcacg cagtgacgca gatcgtgcgg tgtccaccta tcgcagcagc gaagaagagc
    13621 tgcattccgc ggagtttggt gcgcgtgtag ctgaacacga gctgacgctc gcccagtcgg
    13681 cgctcatgcg gctcagcggt aagggagagg gcgaggagct cgacatcatc tcgccggtcg
    13741 ccggtcaggt tctcaagatc tatcaggaaa gcgaaggtgt cgttcaaggg ggcacaccga
    13801 tactcgaggt cggagatccg gcggcgctcg aagtcgtcgt cgacgtcctg agccaggatg
    13861 caacacggat tcccattggc gcgtcgacca cgatcgaacg ttgggggggc aaggtcccgt
    13921 tgcgcagcca tgtgcgtgtc gtcgaaccct cggcgtttac gaagctttca gcgctcggcg
    13981 tggaagaaca gcgcgtgaac gtcatcatcg acatggatga agaccatgag cgctggtcca
    14041 gtctgggcga cggatatagg gtcgaagcac gaatcacggt ctgggagggc aaggatgtct
    14101 tgaaggttcc tgcgagcgcc gtattccgtt ccgacgaagc ctgggcgaca ttcgtcgtcg
    14161 atgagggcgc tgcgatcatc cgaaccgtcg agctcggtga aaccaacggt ctcgagaccg
    14221 aggtcctttc gggtctcgat gaaggcgagt cggtgatcgc ctacccgagt gacagcgtcc
    14281 gcgacggtgt gtcggtcaag gccaggtgag cggagcatga acccgatcat cgattcaaca
    14341 acgctcaccg aggttttcca ggatcgccag atcatgagtc atctggtcgc gacgctggtc
    14401 ctgctggtct cggtcctgtc gatccgctac gtggccctcc gtttcgtgcg tcgggccctc
    14461 ccgagcaagg acaagctgcg actgcgctgg tcctcccaga ttcgcggcct ttcctacgcg
    14521 attctggcat tcggcctgct cacgatttgg gcacaagagc ttcaaaccct cgcggtttct
    14581 ttcgtcgtcc tcgcgatggc catcgtctgg gcgaccaagg aaacgatcgc ctgcgttcag
    14641 ggcgccgtgt atcgcgtgag ctccaacgcc ttcgaggttg gtgaccgaat cgccgtcggg
    14701 tcggttcgcg gagacgtcat cgacgccggg ctgctcagca cgctggtgct cgaggtaggg
    14761 caagggcacc agcggaccgg gcgtacgatc agcataccca atagcctcct catgaccgag
    14821 cctgtgctca acgagagtct tgcgggtgag tacatgctgc acttgatgag cattcctgta
    14881 gatcgcaacg ccgacctcgc cgcgatcgag cgcaaggcgc tctctgccgc cagggatgca
    14941 tgcgcagagt tcatcgacga cgttcgtcgg ccgatcgcgg ctcgctatcg ccgtcatggc
    15001 ctggtcccgc ccatcgtcga tccgcgggtc acctatcaag tcgtcgacaa aaataccgtc
    15061 aacatcatgc tgagaattcc aacaccgagt cgcctggaac ggcaggtcga acaacgcgtt
    15121 ctgagagcgg ttctcggtgt tccaaaggac cgcgacacgc tcccaccgcc ttcacgatga
    15181 acagcgacga cctagaaatc cgaatcgccc ggctcgaagc catcgaagcc atcaaggcgc
    15241 tcaaggcgcg ctactggcat tgctgcgata cgaaagacgt cgaaggcgtt cgcgattgct
    15301 tcctcgatgg gccggtggag attcactacg acggccccgt tggtctcgtg aaccatcggg
    15361 acgggctcta ccaggtgttc aaggacgtcg cttgccagac ccagatcgtc gagatgcacc
    15421 acggcgggcc gccaaacata gaggttcagg gcccggatcg tgcaagcgga cgctggggcc
    15481 tggtctatca tttgatgaat accgacctgc gaaccgtgag cgtggtgggc ggctactacg
    15541 acgacgaata tacgcgcgtc gatggcaagt ggtggatcag caaggcgcgc ttcgaggtat
    15601 gttcggccgt cacgtacggt tggaaagacg cgggactccg aatgctccac gcggccgcgt
    15661 tccttcctga ggccaagtga aggggcggtt gccccggccg ggctgatttg ccaaggtgcg
    15721 ggcgcgatgg aagaaatcag gcctgtcgat gggctccgct gtctggtaac cggcagcgcc
    15781 ggcttcgtcg gaagcaattt ggttcgggcg ctcctcgagc ggggctgtga agtgcatggg
    15841 ttcgaccggg ccccagcaac gttcgaggat ccgaagctgc gttggtttcg tggagacatc
    15901 cgcaacagag acgcgttgag cgaggccatg aaaggcgtcg acaccgtctt tcacaccgct
    15961 gccatgatcg aaacgctcac ctacacacct agaccattcg cggagcaggt tcgtagcgtg
    16021 aacatcgaag ggacgcgcag cgtgcttcag accgcccgcg actccggtgt acgacgtctc
    16081 gttcacacca gctcgatcat cacggttttc ggtgacggac actcgggcgt cgacgagacc
    16141 gcgccataca gcaccgcttc ggatctctat tcgagcacga aagtcgcatc cgaacagctc
    16201 gtcctggctg ccaatggcga gtccggcatg ttcacgtgcg cccttcggcc gggaggaatt
    16261 tacggaccgg gcgagcgtaa cacgctgatc ggtccaatga tcaaggcgct caagcagggc
    16321 gtgcccatcg tcgtgttcgg agatggggcg acgcgcatgg actacaccta catcgacaat
    16381 ctggtcgatg cgcagattcg tgccgcagaa cgcctcgtcg aagggtctcc cgtatgcggc
    16441 gaggcttact tcgtcaccga cggcgatccg atcaacacgg gggacttcag tcagacgctc
    16501 gtcggtcaga tgggtctcga cgcacggaca cttcgagtcc caggtcggct ggcgcgggcg
    16561 attgcaacag cgggcgagcg cgtgtttcag cttctcggca agccaaagcc gcccgtgagt
    16621 atcgtcgacg tcgagctttg tgtgcacgac agctatttct cgatcgacag tgcgcggcgt
    16681 gatctgggat ac
//
LOCUS       DASRZF010000338         6116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_951035, whole genome shotgun sequence.
ACCESSION   DASRZF010000338 DASRZF010000000
VERSION     DASRZF010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 6116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6116
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951035"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VFG22_15055"
     CDS             <1..218
                     /locus_tag="VFG22_15055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417614.1"
                     /translation="RGQPPVLFAHVDESWELLSTESRQAEAELLFKTAHQRWGTRDGF
                     IHRGEALVAQCWGKKVTVFGSLHGDSK"
     gene            215..625
                     /locus_tag="VFG22_15060"
     CDS             215..625
                     /locus_tag="VFG22_15060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HET6417615.1"
                     /translation="MNASARISNKRRRQAGYTLIDMMTVVLIISVLGGLGFNQYNKYV
                     ARARRPETVMAFRAIAAAQREYLLLHGKYAGTFGELGFRMERGKLISPTEIQGLTYSY
                     RIIQDQGPRSWYVVATGNIDGDPFPDIVAASNPR"
     gene            703..1182
                     /locus_tag="VFG22_15065"
     CDS             703..1182
                     /locus_tag="VFG22_15065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417616.1"
                     /translation="MKAKRWTLGVVLAAAVSLVGLVGAGASPFSSYILSDDLGNSVRG
                     SAPEASEWDDGSGAEDGTAGGGASADTGSDGTGKSNNGHGNNADGVDSSNPGQGKGGP
                     NGAADASCPEGGTCVDDETKGGGTTSADTTTDTSTDTSTDTSTDTKTNNGKANGKKK"
     gene            complement(1237..2853)
                     /locus_tag="VFG22_15070"
     CDS             complement(1237..2853)
                     /locus_tag="VFG22_15070"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET6417617.1"
                     /translation="MGDTRKRSFWGWGWADKFPDANARKAIAEHAQLMLGFSPKRLDE
                     PPALESIELSRARLSPPGSLAHSISADHEARVRHTYGRNYRDVMRGFRGDFAAAPDLV
                     ASPADEAEINDVLDWASRAKVAVVPFGGGTSVVGGIEADVDDRYQGVLSLDLRRLDRV
                     VEVERSSLSARIQAGATGPVLETQLAEHGLTLRHFPQSFEFSTLGGWLATRAGGHFAT
                     VYTHIDDLTQSIRAITPRGPWESRRLPGSGAGPSPDRWMLGSEGIFGVITEAWMRVRP
                     RPQFRATATTHFADFRRAVEAVRALSQSGLYPSNCRLLDKREAALNGVTMDGTHVLIV
                     AFESADHPVRPWMDRALELVADHGGACPKGPQHRERGERSDADDGAGAWRDAFIDAPY
                     LLNVMASMGVIVDTFETACTWDRFEELHGGVIAAVREAMKETCGAGFISCRFTHVYPD
                     GPAPYFTFVAPGRFGAEIDQWMTIKKAASDAVISHGGTITHHHAVGRVHRPWYERQRP
                     PLFGDALLAVKRVLDPDGVMNPGVLVDPQR"
     gene            2932..3759
                     /locus_tag="VFG22_15075"
     CDS             2932..3759
                     /locus_tag="VFG22_15075"
                     /inference="COORDINATES: protein motif:HMM:NF004196.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6417618.1"
                     /translation="MSFPKPLRAVVTGAGSGLGRAMALDLAARGASLIVSDINASSAE
                     ATAELVRAQRAVAESIPCDVTDRDAVFALIEETERRLGGLDLIANNAGVAVGGPFDEI
                     SIEDWRWAVDINLWGVIYGCQAAIPRMRAQKRGYIINVASAAGLLAPPGMSAYNVTKA
                     GVVSLSETLYAEYKPQGIRVAVLCPTFFRTNIVESGRGATTEKEDAQVIRWMERSKVQ
                     AADVAKEAIDAVRDGKLYVQPMRDGRMAWRLKRTAPQRFYDSLSRAHEKFLRSRSKR"
     gene            3802..4638
                     /locus_tag="VFG22_15080"
     CDS             3802..4638
                     /locus_tag="VFG22_15080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417619.1"
                     /translation="MRPTHTWLLLIALLSVATSASRARAEDVADEERVIAELEEPGAP
                     GIVAQEPDAFDRERLAYNATILDELLTRASEQTIREERFAAAAAITGGSILLGLGTWR
                     LVEKDPQSQFSRGLGVMFLTLGMADFTTGIFAATRIAHERHRLDRWKHARRQGITAVE
                     LAHFEGELQAAQEMRQGERLLVRWNAFTHAFAGVLVLALTPIPDSMSGADRVSGYVIG
                     GVFFAVGVATFAASFRPTPSEKAWNDYQKRRMPTRGRELSWGVAPSLSRSGVGVAFGG
                     AF"
     gene            4687..5430
                     /locus_tag="VFG22_15085"
     CDS             4687..5430
                     /locus_tag="VFG22_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6417620.1"
                     /translation="MDLDQVKVIITGGASGLGEHFAKQLLAAGARVAVGDVNEEGLAA
                     LPKGIHRRRLNVGNPDEFADFVAWAAEQMGGLNSLINNAGIIRDGLWVGKSRSTGEIR
                     RMSREDWDLVIAINLTGASLMVQEVVAHMIERGEPGVIVNISSIARHGNRGQTNYVAA
                     KAALAANTRTWMKEFAPYGIRVAAVAPGATETAMTAGMNQKALDMIRASVPLGRMGQP
                     EDIWRAVKFIYDCDYFTGETIDVHGGAAL"
     gene            complement(5435..5767)
                     /locus_tag="VFG22_15090"
     CDS             complement(5435..5767)
                     /locus_tag="VFG22_15090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417621.1"
                     /translation="MVFVLNTLVSALVIGAAAWLSRRNPETAGFLIALPLATMLALPL
                     AYLQHRDPVSAFQMARSILVALPITLLFFVPFLMRDRFSFWGAYALGCALLPLGYWAH
                     GAIMRALG"
     gene            complement(5786..>6116)
                     /locus_tag="VFG22_15095"
     CDS             complement(5786..>6116)
                     /locus_tag="VFG22_15095"
                     /inference="COORDINATES: protein motif:HMM:NF028121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HET6417622.1"
                     /translation="LDPDFMFMIGKMTMGVLAASEFPPDMDRAKLILDEVLEKTERRN
                     LMVQMSMARYYAVNAGDSQLFRDLLEEVVEAGDVLPEARLSNAIARRRAQRYLDRIEY
                     YFSDLSE"
BASE COUNT         1211 a         1939 c         1948 g         1018 t
ORIGIN      
        1 ctcgaggtca accccccgtt ctcttcgcac atgtggacga gtcctgggag ctcttgtcca
       61 ccgaaagcag gcaagcagaa gccgaattgc ttttcaagac cgcacatcag cgctggggaa
      121 cgcgggacgg tttcatacat cgtggcgaag cgttggtcgc gcagtgctgg ggcaagaaag
      181 tcacggtgtt cggaagcctt cacggagatt caaaatgaac gctagcgcgc ggatatccaa
      241 caagaggcgg cggcaagctg gctacacgct catagacatg atgaccgtcg ttctcatcat
      301 cagcgttctg ggaggtctgg ggttcaacca gtacaacaag tacgttgccc gagcgcggcg
      361 cccggaaacg gtcatggcgt ttcgcgccat cgcggcagct cagagggaat atctcctcct
      421 ccacggaaag tacgcgggca cgtttgggga gctcgggttt aggatggaac gcgggaagct
      481 gatctcgcca accgagattc aaggcctcac ttacagctac cgcatcatcc aggaccaggg
      541 ccctcggtcc tggtacgtgg tcgccacggg caatatcgac ggggatccgt tcccggacat
      601 cgttgccgcg agcaacccac ggtaatctga acggtcggta atatacccca cttgaggact
      661 gtaaatttct tcatatactg aaatatgaaa ggggcatctg aaatgaaagc aaagcgttgg
      721 acactagggg tcgtgcttgc ggcggctgta agtcttgttg gactcgtagg ggccggcgca
      781 agcccatttt cttcctacat tctgagcgat gatctcggga actcggttcg gggcagcgct
      841 ccagaggcga gcgagtggga tgacggatcg ggcgcagagg acggcaccgc cggcggtggc
      901 gccagtgctg acaccggcag cgacggcaca ggcaagagca acaatggtca tggcaacaac
      961 gccgacgggg tcgactcgag caacccgggg caaggcaagg gtggcccgaa cggcgcagcg
     1021 gacgcatcct gcccagaagg cggaacctgc gtcgacgacg agaccaaggg cggcggcacg
     1081 acgagcgccg acaccacgac cgacacgtct acggacacgt ctacggatac gtcgaccgac
     1141 accaagacca acaacggcaa ggcgaacggc aagaagaagt agcctcaccg cttgattgga
     1201 acctcaggaa ggcgccctac ggggcgcctt cttcttttaa cgctggggat cgaccaggac
     1261 gcccggattc atcacgccgt ccggatcgag aacgcgcttc accgcgagga gcgcgtcgcc
     1321 gaacagcggc ggacgctgcc tctcgtacca gggccgatgc acacgaccca cggcatggtg
     1381 atgcgtgatc gtgccgccgt gcgagatgac cgcgtccgac gcggccttct tgatcgtcat
     1441 ccattgatcg atctcggcgc cgaagcgtcc gggcgcgacg aaggtgaagt acggagccgg
     1501 gccatcgggg tagacgtgcg tgaacctaca actgatgaag cccgccccac aggtctcctt
     1561 catcgcctct cgaaccgcag cgatcacgcc gccgtgtagt tcttcgaaac ggtcccaggt
     1621 gcatgcggtt tcgaaggtgt ccacgatgac ccccatgctc gccatgacat tgagcagata
     1681 cggcgcatcg atgaacgcgt cccgccaagc gcccgcaccg tcgtccgcgt cgctgcgctc
     1741 tcctctctcg cgatgctgag gccccttcgg acaggcgccc ccatgatcgg cgacgagctc
     1801 gagggcgcgg tccatccagg gtcgtacggg atgatccgca gattcgaagg ccacgatcag
     1861 cacatgggtt ccgtccatcg tgacgccgtt gagcgccgcc tcgcgcttgt cgagaagcct
     1921 gcaattgctg ggatagagcc cggattgcga aagcgctcga accgcctcga ccgcgcgccg
     1981 aaagtcggcg aagtgggtcg tggcggtcgc gcggaactga ggtcgcggcc gaacccgcat
     2041 ccatgcctcg gtgatgacgc cgaagatccc ttcggagccc agcatccatc ggtcggggct
     2101 cggcccagca ccggagccgg gcaagcgtcg agactcccag ggtcccctgg gcgtgatcgc
     2161 tcggatcgac tgcgtgaggt cgtcgatgtg cgtgtagacg gtagcgaagt gaccacccgc
     2221 gcgagttgcg agccaaccac cgagcgtcga gaactcgaac gactgcggga agtgacgaag
     2281 cgtgaggccg tgctccgcaa gctgcgtctc cagcaccgga ccggttgcgc cggcctgaat
     2341 acgcgcggag agagagctcc gctccacctc gacgacgcga tcgaggcgcc ggaggtccag
     2401 gctgagcact ccctggtagc gatcatcgac atccgcttcg atgccaccca ccacgctggt
     2461 ccctccgccg aacggaacta cggcaacctt ggcccggctc gcccagtcga gaacgtcgtt
     2521 gatctccgct tcgtccgctg gactcgcgac gagatccgga gcggcggcga agtcacccct
     2581 gaagccacgc atcacgtcgc ggtagttgcg gccgtaggta tgccggacgc gcgcctcgtg
     2641 atcggcgctg atcgaatgcg cgaggcttcc gggcgggctc aggcgcgcgc ggctcaactc
     2701 gatcgattcc aatgcgggtg gctcatcgag ccgcttcggc gagaagccga gcatgagctg
     2761 agcgtgctcg gcgatcgctt tgcgcgcatt tgcgtccggg aacttgtccg cccagcccca
     2821 gccccagaaa ctacgcttcc gtgtgtcgcc catggaccgc gaccttccac catcgcgcca
     2881 tcggtttgca agcgccttga cggacccccg gatcgaggct tccatccatg aatgtctttc
     2941 cccaagccgc ttcgagcggt ggtcaccggg gccggcagcg gtctcggccg tgccatggct
     3001 ctcgatctcg ccgcgcgcgg cgcttccctg atcgtctccg atatcaacgc tagctctgcg
     3061 gaggcaaccg ccgagctcgt tcgcgcacag cgagctgtgg cggagtccat cccctgcgac
     3121 gtcaccgatc gagacgccgt gtttgcgctc atcgaagaga cggagcgacg cctcggagga
     3181 ctcgatctaa tcgccaacaa cgccggagtg gccgttggcg gaccgttcga cgagatctcg
     3241 atcgaagact ggcgctgggc cgtcgacatc aatctctggg gcgtgatcta cggctgccaa
     3301 gcggcgattc ctcgaatgcg cgcgcagaag cgcggctaca tcatcaacgt cgcctcggcc
     3361 gcagggctgc tcgcccctcc gggaatgtcc gcctacaacg tgaccaaggc cggcgtggtg
     3421 tcgctgagtg aaacgctcta cgcagagtac aagcctcagg gcatccgtgt cgccgtgctc
     3481 tgtcccacct tcttccgcac gaacatcgtc gaatcgggcc ggggcgccac caccgagaaa
     3541 gaagacgcac aggtcattcg atggatggag cgatccaagg tgcaggccgc agacgtcgcc
     3601 aaagaggcca tcgatgcggt gcgcgacggc aagctctacg ttcagcccat gcgagacggg
     3661 cgaatggcgt ggcgtctcaa acgcactgct ccgcagcgat tttacgattc gctcagtcga
     3721 gcgcatgaga agtttctccg aagccggagc aagcgctaat tggccatggt cggggggcgc
     3781 tgctaaccag actggacgcc catgcgaccg actcacactt ggctgcttct catcgcccta
     3841 ctctccgtgg cgacctctgc cagcagagct cgggccgagg acgtcgcaga cgaagagcga
     3901 gtcatcgcag agctcgaaga gccgggggcc ccgggtatcg ttgcccagga gccggatgcg
     3961 ttcgatcggg aacgattggc gtacaacgcg acgattctgg acgagctgct gacacgagcg
     4021 agcgagcaga cgatcaggga agagagattt gccgccgccg ccgccatcac cggtgggtcg
     4081 atcttgcttg ggctcggcac ctggcgactt gtcgaaaagg atccgcagag ccagttttcg
     4141 agaggcctgg gcgtgatgtt cctgaccctc ggcatggccg acttcacgac aggcatcttt
     4201 gccgcgaccc gcatcgcaca tgagcgacat cggctcgacc gatggaagca tgcgcggcgc
     4261 caagggatca ccgccgtcga gctggcgcat ttcgaaggcg agctgcaagc ggctcaggag
     4321 atgcgccagg gcgaacggct cctggtccgc tggaacgcct tcacccatgc tttcgcgggc
     4381 gtcctcgttc tcgccttgac gccgattccc gatagcatga gcggcgccga tcgtgtctca
     4441 ggctacgtga tcggcggggt cttcttcgcg gtgggcgtcg ccaccttcgc cgcatcgttt
     4501 cgcccgacac caagcgagaa ggcttggaac gactaccaga agcggcgaat gcccaccaga
     4561 ggccgggagc tcagctgggg agtcgcccct tcgctctcgc gcagcggagt cggcgtcgca
     4621 ttcggcggcg cgttctagcc cgaccggctt gcgcggcggc tgcatcagcg agtagtaagg
     4681 gccctcatgg acctcgacca agtcaaagtc atcatcacgg gaggcgcatc cgggctcgga
     4741 gagcacttcg ccaaacagct actcgccgcg ggcgctcgag tcgccgtcgg agatgtcaac
     4801 gaagaaggcc tcgcggcgct tcccaaaggc attcaccgaa gacgactcaa cgtgggcaac
     4861 cctgacgaat tcgccgactt cgtcgcctgg gcggccgagc agatgggcgg cttgaacagt
     4921 ctgatcaaca acgcgggcat catccgcgac gggctctggg tcgggaagag tcgcagcacg
     4981 ggtgaaattc gacggatgtc gcgggaggac tgggacctcg tgatcgcgat caacctcacg
     5041 ggcgcttcgc tcatggttca ggaggtggtg gcccacatga tcgaacgggg cgagcccggc
     5101 gtcatcgtga acatctcctc gatcgcccgt cacggaaacc ggggtcagac caactacgtg
     5161 gccgccaagg ccgccctcgc cgcaaacacc cgcacctgga tgaaggagtt tgctccttat
     5221 ggcattcgtg tagcggccgt cgcacctggc gccaccgaaa cggccatgac ggccggcatg
     5281 aaccagaagg cgctcgatat gatccgcgca tcggttcctc tcggccggat gggccagccc
     5341 gaagacatct ggcgagcggt gaagttcatc tacgattgcg actacttcac cggcgagacc
     5401 atcgacgtgc acggcggcgc cgcgctctaa ctaatcaacc gagcgcgcgc atgatcgcgc
     5461 cgtgcgccca gtacccgaga ggcaagagcg cgcatccgag cgcgtaagcg ccccagaacg
     5521 agaatcgatc gcgcatcagg aacggaacga agaacaggag tgtgatcggc agcgcaacca
     5581 gaatgctccg cgccatctga aacgcactca ccgggtcccg atgttgcaag tacgcgagcg
     5641 gcaaggcgag catcgtcgcc aatggcaggg cgatcagaaa cccagcagtc tccggatttc
     5701 gacgtgagag ccacgctgcg gcgccgatga cgagtgccga tacgagcgtg ttcaagacga
     5761 agaccatgtc ggctcgccta agcgctcatt cgctgagatc gctgaagtag tactcgattc
     5821 gatcgaggta gcgctgggcg cggcgccgcg cgatggcgtt cgagaggcgc gcctcgggga
     5881 gcacgtcgcc cgcttcgacg acctcttcca gaaggtcgcg gaaaagctgc gagtcgccag
     5941 cattgacggc gtagtaacgg gccatgctca tttggaccat caagttgcgt cgctcggtct
     6001 tctcgaggac ctcgtcgagg atcagcttgg cgcggtccat atcgggcggg aactccgagg
     6061 ccgccagcac gcccatggtc atcttcccga tcatgaacat gaagtccgga tcgagc
//
LOCUS       DASRZF010000339         4622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3695681, whole genome shotgun sequence.
ACCESSION   DASRZF010000339 DASRZF010000000
VERSION     DASRZF010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3695681"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2022)
                     /locus_tag="VFG22_15100"
     CDS             complement(<1..2022)
                     /locus_tag="VFG22_15100"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HET6417623.1"
                     /translation="MVRLTNILEKVKGYHPRADTDLINKAYVYAARMHDGQTRKSGDD
                     YFVHPVSVADIIADMRLDASSVCAALLHDVVEDCEVSLSEIETVFGDEVAFLVDGVTK
                     LGKVNFASREDRQAESFRKMLVAMARDIRVLLVKLADRLDNMRTLEHMSPSSQERIAR
                     ETIEIYAPLAGRLGIQWLKAELEDLSFKHLEPDAYEVLDEQVRGVAKSTDVYITDVTA
                     KLKKMLLTRGFSVQINGRRKHLYSLHRKMKSSGIGFEQVHDFVAFRVITESVADCYAT
                     LGVIHSEWTPVPGRFKDYIALPKSNMYQSLHTTVIGPGNRRVEIQIRTHEMHRTADYG
                     IAAHWQYKEHTGGIDPKDAARFVWLRQLMEFQKEVADPEEFYESVKVDLFPEDVYVFT
                     PKGDVMTFPRGATPVDFAYAVHSEVGHRCSGARVNGAIVPLRHKLQNGDVVEIITSKN
                     HHPSKDWLEFVITSRARSRIRFYLRTEERKHAIKLGKELLEKEMRKRDMSLGRFLKSK
                     HVKEVLARFRVGTEEGLYAKIGIGKLALPTVMEAIVPSEDTERDSLRPGFIERTVQRV
                     AGDDEAAGIRIQGLDNMLVRFAQCCSPVPGDPVTGWITRGRGVSVHRRGCPRVLELDP
                     ERRVQVTWADDVEVDLPVSLRIMTDDRAGILASVSSVFSANGVSISEASC"
     gene            2200..2511
                     /locus_tag="VFG22_15105"
     CDS             2200..2511
                     /locus_tag="VFG22_15105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417624.1"
                     /translation="MASQLAGVWPREVEIEYLLEEGIQAVDVDYVQEGETVTSARFTK
                     AALKGSNVRMSVRLQPGEYLALVTVYRTDGIGVEQSRKLVVPAQGVTRFDLREASGGS
                     D"
     gene            2514..>4622
                     /locus_tag="VFG22_15110"
     CDS             2514..>4622
                     /locus_tag="VFG22_15110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET6417625.1"
                     /translation="MLGAENARQPKRMAVLFTALGATVALTLWMANREPFAHGPLWAA
                     LIALLAGLSWVSWLVPPAAGAVLSLQETAFGKQKGEPWSPVTGAGLALLTLVLGAWLG
                     GYEWLPVVILLSLACLLPSALRRPGLAVMIAIAVLYLPLLGTTSLWDPWETHYGEVAR
                     EILARNDWISLWWAQDKWFWSKPVLLFWMEALSMGFLGVDFMPDANPANPEWALRLPA
                     VLMALAALAVLYATVRRYFGRRAGALAVLVTATMPQFFFISHQAITDLPLVAAITIAI
                     CCLLVALEEDPAREATVFQIGGWRVSFQHLVLLGILMLVLPQALYLISRNISWIEGHG
                     LVAHADRFLYGSAGNIDVPGNPTLHDQGPRVRGLGGQPFVQGVLWLTVLSGLTLILRK
                     ERRIRRLLMASFYFFCAIAFMAKGLPGLAIPGAAALFYLIASRRWSLLTSGELQVSRG
                     ILIVTVVSMPWYLAMYVRHGPAFTNRLLIHDHINRLASAVHGDTGTIEYFVGQIGYAT
                     FPWIALIPAALLLGAWIRSRSERDGSREALLFIAMWLISSFVLFSVMVTKYHHYILPA
                     LVPAGVLIGIAMAECWGPRRPLATLLAAASGCCAILGFAWLLGDPRGVIPADAMGVED
                     WILQQANPVRAYGLLGLGLILALLARSGTAKAETRLAPARWSTALGVALLSGACVLAF
                     VGRDLSWITSARPQGNERLIH"
BASE COUNT          785 a         1451 c         1429 g          957 t
ORIGIN      
        1 gcacgaggcc tcactgatgc tcaccccatt tgcggagaaa accgacgata cggaggccag
       61 aatccccgcc cgatcgtccg tcatgatgcg caaagagacc ggcagatcca cctcgacgtc
      121 gtcggcccat gtgacctgca cgcggcgctc tgggtcgagc tcgagaacgc gcgggcaacc
      181 tcttcgatga acggaaaccc cgcggccgcg ggtaatccaa cccgtgacag ggtctccagg
      241 cacgggacta cagcactgcg caaaacgcac gagcatgttg tccaagccct gaattcgaat
      301 tccggcggcc tcgtcatcgc ccgcgactcg ctggacggtc cgctcgatga agcctgggcg
      361 caggctgtcg cgctcggtgt cttcgctcgg tacaatcgct tccatgaccg tgggtagcgc
      421 gagcttgcca atgccgatct tcgcgtagag cccttcttcc gtgccgacgc ggaaccgtgc
      481 aagcacctct ttgacgtgct tgctcttcag gaatcggccg agcgacatgt cgcgcttccg
      541 catctctttt tcgagcagct ctttgccgag cttgatcgcg tgtttgcgct cctcggtgcg
      601 caagtagaat cgaattcggg atcgtgcgcg gctggtgatc acgaactcga gccagtcctt
      661 gctagggtgg tggttcttgc tcgtgatgat ctcgacgacg tcaccgtttt gcagcttgtg
      721 gcgtagggga acgatcgcgc cgttgactcg ggcacccgag caccggtgac ccacctcgga
      781 gtggacagcg taggcgaaat cgacgggtgt agcgccgcga gggaacgtca tgacgtcgcc
      841 cttgggcgtg aacacgtaga cgtcctccgg gaagaggtcg accttcacgc tctcgtagaa
      901 ctcttcggga tcggcgactt ccttctggaa ctccatcagc tgacgaagcc agacgaatcg
      961 cgcagcgtcc ttcggatcga tgcctccggt atgctctttg tactgccaat gggcggcaat
     1021 tccgtagtcc gcggtgcggt gcatctcgtg ggttcgtatc tggatctcca cgcgccgatt
     1081 acctgggccg atcaccgtcg tgtgcagcga ctgatacatg ttggacttgg gaagtgcgat
     1141 gtagtccttg aaacgacccg gaacgggggt ccattccgag tggatcacac cgagcgtggc
     1201 gtaacaatcc gcgacgctct cggtgatgac ccgaaacgcc acgaagtcgt gcacctgctc
     1261 gaacccaatg ccactcgact tcatctttcg gtgaagcgaa tagaggtgct tgcggcgccc
     1321 gttgatctgc acggaaaatc cgcgcgtcag caacatcttc ttcagcttcg cggtgacgtc
     1381 ggtgatgtag acgtcggtgc tcttcgcgac ccctctgacc tgctcgtcca gcacctcgta
     1441 agcatctggt tcgagatgct tgaagctcag gtcctcgagc tccgccttga gccactgaat
     1501 gcctagacgt cctgcgagcg gcgcatagat ctcgatcgtt tcccgggcga tgcgttcctg
     1561 cgaggagggc gacatgtgct cgagcgttcg catgttgtcg agtcggtccg cgagcttgac
     1621 caagagcacg cgaatgtcgc gcgccatggc gacgagcatc ttgcgaaagc tctccgcttg
     1681 acggtcttcg cgcgatgcga agttgacctt gccgagcttc gtcacgccat cgacgaggaa
     1741 cgcgacctcg tcgccgaaca ccgtctcgat ctcggacagg ctgacctcgc aatcctcgac
     1801 gacgtcgtgc agtagcgcag cgcagacgct cgaagcatcg agccgcatgt cggcgatgat
     1861 gtccgccacg gagactgggt gaacgaaata gtcgtcaccg gacttgcgcg tctgaccgtc
     1921 gtgcatgcgg gccgcataga cgtaggcctt gttgatcaga tcggtatccg ctcggggatg
     1981 gtatcccttc accttttcaa gaatattggt gaggcggacc atagcggcgc actaacgggc
     2041 aaacctcaat ttaacaccgt ctccaattgg tgccaaggtt gtgacccttc ctggacttgc
     2101 gtaaaagcct gtttcgtcat gagcttaaag ctaaacatag ggccatcgtc aacgaagcgc
     2161 aggatcgcga ccgcagtagc catagccggg ctgattctgg tggcaagcca gctggcaggc
     2221 gtctggcccc gcgaggtcga gatagaatac ctgctcgaag agggtatcca ggcggtcgac
     2281 gtggattacg ttcaggaggg cgagacggtg acgagcgccc ggttcaccaa ggcggccctc
     2341 aagggttcca acgtgcgaat gagcgttcga cttcagcccg gcgagtatct ggcgctggtc
     2401 acggtctatc ggactgatgg aatcggcgtc gagcagagcc gaaagcttgt cgttccggcc
     2461 cagggggtca cccgcttcga cctcagagaa gcaagcggcg ggtcagattg agcatgctgg
     2521 gcgcggagaa cgcgaggcag ccgaagcgga tggcggtgct gttcacggcc ctcggcgcca
     2581 cggtcgccct gacgctctgg atggccaacc gcgaaccgtt cgcgcacgga ccgctctggg
     2641 ctgcgctcat cgcccttctt gcaggcctgt cctgggtatc ctggctggtc ccgcccgcgg
     2701 cgggcgcggt gctttccttg caggaaaccg cctttggaaa acagaagggc gagccctggt
     2761 ctccggtcac cggtgcgggt cttgcgctcc tcacactggt cctaggcgct tggctgggag
     2821 gctacgagtg gctgccggtc gtcatcttgc tcagcctcgc ctgcctcctc ccatcggcgc
     2881 ttcgacgtcc gggcctggcc gtgatgatcg cgatcgccgt gctctacctg ccactgctcg
     2941 gcaccacgag tctctgggat ccatgggaga cccactacgg cgaggtcgcc cgggaaatcc
     3001 ttgcacgaaa cgactggatc agtctctggt gggcccagga caagtggttc tggtcgaagc
     3061 cggtgctgct cttctggatg gaagcgctga gcatggggtt cctcggtgtc gacttcatgc
     3121 cggacgcgaa tccagcaaac ccagaatggg cgctgcggct ccccgccgtc ctgatggctc
     3181 tcgccgcctt ggcggttctc tacgcgaccg tgcggcgcta cttcggccgg cgagccgggg
     3241 cgcttgcggt cttggttacg gcgacgatgc cccagttctt cttcatctcg catcaggcga
     3301 tcaccgactt gccactggtc gctgcaatta cgatcgcgat ctgctgcttg ctcgttgcgc
     3361 tcgaggaaga tccagcacgc gaagcgacgg tattccagat cggcggatgg agggtctcgt
     3421 tccagcacct ggtcctgctc ggcatcctga tgctggttct tccgcaggca ctctatttga
     3481 tctcccggaa catctcgtgg atcgagggtc acggcctggt cgcccacgcc gaccggtttc
     3541 tctacggttc ggcgggcaac atcgacgtcc ctggaaaccc gaccctgcac gaccagggcc
     3601 cgcgtgtccg cggtctcggc ggccagccct tcgtgcaggg ggtcctctgg ctgaccgtac
     3661 tctccgggtt gacgctcatc ctccgcaaag agcggcgcat tcggcgtctg ctgatggcct
     3721 cgttctactt cttttgtgcg atcgcattca tggccaaggg cctcccgggg ctagcgattc
     3781 ctggagctgc tgcgctcttc tatttgatcg cctcgcgacg ctggtcgctt ctcaccagcg
     3841 gtgagctcca agtctcgcga ggcatactca tcgtcactgt ggtgagcatg ccctggtacc
     3901 tcgccatgta cgtgcggcac ggcccagcgt tcaccaatcg cttgttgatt catgaccaca
     3961 tcaaccggct cgcgagtgca gtgcacggag acacggggac catcgagtac ttcgtcggac
     4021 agatcgggta cgcgacgttc ccctggatcg ctctgattcc agcggcgctc ctgctgggcg
     4081 cctggatccg cagtcgctct gagcgcgatg gaagtcgcga ggccttgctc ttcatcgcga
     4141 tgtggctgat ttcctcgttc gtcctcttca gcgtgatggt gaccaagtac catcactaca
     4201 ttctgccggc gctcgttccc gcaggagtgc tgatcgggat cgcgatggcc gaatgctggg
     4261 gtccgagacg tccgctggcg accttgctcg cagcggcgtc aggctgctgc gcgatcctgg
     4321 gtttcgcatg gctcctcggg gacccacgag gtgtcattcc ggccgacgcc atgggcgtcg
     4381 aagactggat cctgcagcag gcgaacccgg tgcgtgccta cggcttgctt gggctcggcc
     4441 tgatcctggc gctgctcgct cgatcgggca cggccaaggc cgagacccgc ctcgcacctg
     4501 ccagatggag caccgcgctt ggcgtagccc tgctctcagg cgcctgtgtg ctcgcgttcg
     4561 tggggcgcga cctcagctgg atcacctcgg cgcggccgca aggaaacgaa cgactcatcc
     4621 at
//
LOCUS       DASRZF010000340         9419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3358271, whole genome shotgun sequence.
ACCESSION   DASRZF010000340 DASRZF010000000
VERSION     DASRZF010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 9419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9419
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3358271"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="VFG22_15115"
     CDS             <1..581
                     /locus_tag="VFG22_15115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417626.1"
                     /translation="KFIGVDSAGLSEQRLALENDYRRAGQRGGELLDMLGSGTASGEA
                     LRIRVAARTATVTQLALAGAFGLQAMLRILARWMRLDPEAVLVVPNLDFADDRMASSE
                     LVQAMTAKTLGAPWSLRSIHVNMQERGLTDMSYEDELEELGREAPLTQGSSDPDGPEA
                     DEPEDDSEDDPNGSDDGESEDPEDEDPKADGE"
     gene            598..1857
                     /locus_tag="VFG22_15120"
     CDS             598..1857
                     /locus_tag="VFG22_15120"
                     /inference="COORDINATES: protein motif:HMM:NF016148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HET6417627.1"
                     /translation="MPLRSKQRKTQLPGPLRTSNDEFFDALLRHQIGLMLFASSLGRR
                     LQVLLDAVEADLRDKVRRGLSRFPASGRGLTTPASVRRLERLLADVRRVRLGALQGVE
                     GLMLEEMSALVLAEPKFVQMAFQTVIPVRLSTGLPAPERLKAIVSVHPFEGKILRDWA
                     RDIRRADIERIEAQIRIGMVQGESIPDISRRITGSARLRGRNGVTEITRRQAQGLART
                     ATNAIATEARNAFFLANREFFDAEIYVATLDSRTTPICRSLDGTRYKVGEGRYPPLHW
                     NCRSIRVPLLDADPLGNRPSKPVHQRELLREFAKQRGIDPPPALRKNLPYGTKGAFDA
                     FARRRTRQMIGVVPAKTTYEDWLRRQPAAFQDDVLGNTKGRLFRRGDLSLDRFVDSTD
                     AEKTLADLARSDAEAFRLAGLDPDDFL"
     gene            1979..2824
                     /locus_tag="VFG22_15125"
     CDS             1979..2824
                     /locus_tag="VFG22_15125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417628.1"
                     /translation="MALKAVHESLDEIPDEYRTLYTEKSGKYELTGIQGVKTQADVDR
                     LSEAIKVERAKTASVKERLAAWGDLDHEEVKRQLDRVPELEAAAKGKLDEDEIERIVE
                     RRVDGTIRSRLSPIERQLKTIEKERDELKSANDAWTQRDRTRKVHDSVLKSAVDAGVE
                     PDVHDDVLLWADRLFEVREDDGQVVTKDGMDVTPGITPADWLSEIQPKKKRIWWGDSV
                     GGGSTGSNGRRVPGFSGKNPFSAEHWNMTEQSRVYREMGRERCEQLAKAAGTTLGGQR
                     PAAKK"
     gene            3587..4573
                     /locus_tag="VFG22_15130"
     CDS             3587..4573
                     /locus_tag="VFG22_15130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417629.1"
                     /translation="MTEQKSRLIQSGALSRSPILDNFLAGGGLTVNVPSFTDLDDDDD
                     NVSTDDVADVIAATAGAAWPSPSLDATPLKITADQEIAVRLSRNNSWSSADLAAALAG
                     ADPMSSIADRVAFYWSRKLQKAYVATWNGVIKDNTANDSGDYTNDISGGSFVDGVTNF
                     SAEAFIDATATLGDSSDDLGIMMVHSIVKARMKKNNLIDFIPDSEGRVNIPTFQGYIV
                     IEDDGMPVTSSVYDTWIFGSGATMLGTGSPKVPTEAYREPLAGNGGGEEILTNRVEWC
                     IHPIGHAYTGTAPNGGPGNGTGANQLNVAGSWNRVYPERKQIKFARLVTREA"
     gene            4597..5418
                     /locus_tag="VFG22_15135"
     CDS             4597..5418
                     /locus_tag="VFG22_15135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417630.1"
                     /translation="MTTQALFEAAMGGSLIPLKEIEFARTSGDICTATAHGLATGAGP
                     FKVTTTNADAPSGITVAAHSSLAYTPSTDVEDETVTVAGKVYTWKNAPSADGEVDVNA
                     DDKIAAENLAAAINLEPTVGTSYGAAMTANPTVFANVAPDTAVCTVYAKTLDATVGDA
                     IAVAESASGAWAGAGTVLANGASGTDYYVISLTDDTFSLATTKALAIAGTAVTLADAG
                     TGTHFLVMTAKALATGLNDVLTNFLTATGTRCIPAADNVANFWATAIDGVAVHRS"
     gene            5421..5942
                     /locus_tag="VFG22_15140"
     CDS             5421..5942
                     /locus_tag="VFG22_15140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417631.1"
                     /translation="MTKGLPYSMSRGAPLRQEIIKQTVTVRDAAVTVDGATGVGFGSV
                     VIGDIPEGNILILGAVAYMQFTGPTSASLDDDWVGDYGIGSTPADDATITTTDINIIG
                     STAIAAATAEASPRTRGTSNTTDLDHILDNTDGSLEINLTLLVDDANIGADGLDFTVN
                     GELTLAYIMLLDD"
     gene            6003..6701
                     /locus_tag="VFG22_15145"
     CDS             6003..6701
                     /locus_tag="VFG22_15145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417632.1"
                     /translation="MNLLQALESLDPLNSEQWTMQGLPRIEAVSEIMGREVTRMDITN
                     TAPRLVRENARPKAQPATVDTEGNVIGESADAPERVEIEDPKMTAHVGPHDDVVGMPA
                     AQVYGSAELIDRALDEFSRQSDILTARREAIAEKIKELSKRTELLTRARNTLARAGKI
                     KDTATPISDYLKSQQHARAMRAERARRFIDAGTTERDVILGMKGVSPLDAAIAAKNTK
                     AKAPQPLRVSMGQG"
     gene            7365..8027
                     /locus_tag="VFG22_15150"
     CDS             7365..8027
                     /locus_tag="VFG22_15150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417633.1"
                     /translation="MATARRRTLLRVNAIARRKRKDSHRGYPDLHQTHTIAIGTSDAI
                     ANHFDSGDMAGPVTFKFRVDRTSDSATGTVLALGNESTIGFDSGDLEIFTGGGSGDTF
                     TGTFIGAPRDSAEVVVALHPSTGQVRAWVDSVRVIAGTVSTYPWSWAATGTLDYDGIS
                     GLSVRQDLYAYVGQIPRHFFEVSVGGAGSGGVVFDDLLFRASRQATDLATSRFPYMER
                     PD"
     gene            8027..9154
                     /locus_tag="VFG22_15155"
     CDS             8027..9154
                     /locus_tag="VFG22_15155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417634.1"
                     /translation="MGNVVHARRDVSASSSGVTDHGALTGLTDDDHTQYLLEDGSRAM
                     SGDLDMGTNAITNVGNVDGVDVSAHAGDATIHFTEGSIDHTAITNIGTNTHAQIDTHV
                     ADSTIHFIEGSIDHTAIANIGTNTHAQLDTHVADSTIHFTEASIDHTAITNIGTNTHA
                     QLDTHVATSNIHFTEASIDHTAITNIGSNSHAAIDTHIADDEIHFTYAQNSQPSNPST
                     DGVTWLRTGSGAAEIYSWDNTNGFWISFGDWCWMISSAASDVSASPSIAQFVLAKMVQ
                     ITMNDTGKSIRAPFNLQMYRLAMNAQDVPLSSDGIDIKVNAATTTVYWDGTNNYIVQD
                     INATADVTNRIRFQWRFDSTKPAATQRLSAQIYVRRRIPAL"
     gene            9167..>9419
                     /locus_tag="VFG22_15160"
     CDS             9167..>9419
                     /locus_tag="VFG22_15160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417635.1"
                     /translation="MTTDIVAFVTNNDAPLTSPTDEPTIRIRRLDTGALVVTDQDMTE
                     IGDGLWSYSFDESSLLTYGVRVDADPAASSQVTAAERYYS"
BASE COUNT         1890 a         2752 c         3023 g         1754 t
ORIGIN      
        1 caaagttcat tggcgtggac agtgccggac taagcgagca gcgcctggct ctggagaacg
       61 actaccgacg cgcgggtcag agaggcggtg agctgctgga catgctcggg tctggcaccg
      121 catctggcga agcactgcgc atccgagtcg cggcacgcac ggcgacggtg acgcagcttg
      181 cgcttgctgg cgccttcggg ctgcaagcga tgctgcggat cttggcgcgc tggatgcgac
      241 tcgatccaga ggccgtgctt gtggttccga acctcgattt tgccgatgac cgcatggcct
      301 cgagcgagct ggtgcaggcg atgacagcga agaccctggg cgctccgtgg tcgttgcgtt
      361 cgatccacgt caacatgcag gagcgtgggc tcacggacat gagctatgag gacgagctgg
      421 aggagctggg tcgtgaggcg ccgttgacgc agggatcgag tgacccggat gggccggagg
      481 cagatgagcc cgaggatgat tctgaggatg accccaatgg gtctgatgat ggcgagtctg
      541 aggatcctga ggatgaggac cccaaggctg acggtgagta ggtagcggtc tctccccatg
      601 cccctgagat ccaagcagcg caaaactcag ctcccagggc ctctccgcac gtcgaacgat
      661 gaattcttcg atgcgctgct gcggcatcag atagggctga tgctttttgc atcgagcctg
      721 gggcgtcggt tgcaggtgct gcttgatgcg gtcgaggctg acttgcgcga caaggtgcgg
      781 cgtggcctgt cgcgcttccc ggcgagtggt cgcgggctca cgacaccagc gagtgtgcgg
      841 cgattggagc gcttgctggc agacgtgcgt cgagtcaggc ttggtgctct tcagggcgtc
      901 gaggggctca tgctcgagga gatgagcgcc cttgtgctcg cggagccgaa gtttgtgcag
      961 atggcgtttc agacggtgat accagtgcgc ctctcaacgg ggctcccggc gcctgagcgg
     1021 ctcaaggcca tcgtgagcgt gcatccgttc gagggcaaga ttctgcgcga ctgggctcgt
     1081 gacatacggc gtgccgacat cgagcgcatc gaggcgcaga tccgcatcgg catggtgcag
     1141 ggtgagtcga tcccggacat ctcgcgacgc attactggat ctgcacggct gcgcgggcgc
     1201 aatggggtga cggagatcac gcggcgtcag gcgcaaggct tggcgcgaac ggcgacgaat
     1261 gccatcgcga ctgaggcgcg taacgcattc ttcctggcga atcgagagtt cttcgacgcc
     1321 gagatatatg tggcaaccct ggactcgcgc acaacgccga tctgtcggtc tttggatggc
     1381 acgcggtaca aggttggcga ggggcgctac ccgccgctgc actggaattg ccgctcaatc
     1441 agggtgcctc tgctcgacgc ggatcctctg ggcaatcgtc catcgaagcc agtgcatcaa
     1501 cgggagcttt tgcgtgagtt tgccaaacag cgcggcattg atccgccgcc agcgctgcgg
     1561 aagaacctgc cttacggcac caagggcgcc ttcgatgctt tcgcgcgtag gcgcactcgc
     1621 cagatgatcg gggtcgtgcc agcgaagacg acgtacgagg attggctgcg acggcagccc
     1681 gccgcgtttc aggacgacgt tctcggcaac accaagggcc gcctgttccg tcgcggggat
     1741 ctgtcgctgg atcgattcgt tgattcgacg gatgcggaga agactcttgc cgacctcgca
     1801 cgctccgacg cggaggcgtt ccgtctcgcc gggcttgacc ctgacgactt tttgtagtat
     1861 gccgcgcatg gtgcgtttat tgcgcatctt gcacgcgcac gatcaaagcg aggcgggatg
     1921 acccgtccgt gaacacgcgg agcatggctc cgtgcatctg tatgaggagg agaaggccat
     1981 ggcgctcaaa gccgttcacg agtcactgga cgagatcccg gacgagtatc gcacgctgta
     2041 taccgagaag agcggcaagt acgagctgac cgggattcaa ggggtgaaga cgcaggcaga
     2101 tgtggaccgc ttgagtgagg cgatcaaggt ggagcgcgcg aagacagcca gcgtcaaaga
     2161 gcggctggcg gcatggggcg atctggacca tgaagaggtt aagcgacagc tcgacagggt
     2221 gccggaactc gaggctgctg ccaagggcaa actcgacgaa gacgagatcg agcggatcgt
     2281 ggagcgccgg gtggacggca cgatcaggtc tcggctctcg ccgatcgaga ggcagctaaa
     2341 aaccattgag aaagagcgcg atgagctgaa gagcgcgaac gacgcatgga cccagaggga
     2401 tcgaacgcgc aaggtgcatg acagcgtgct gaaatctgcc gtggatgctg gcgtggagcc
     2461 ggatgtccat gatgacgttc ttttgtgggc tgaccgtctc ttcgaggtcc gggaggatga
     2521 cggccaggtc gtgactaagg atggaatgga tgtaacgcca ggcatcactc cggctgactg
     2581 gctctcagag attcagccga agaaaaagcg tatttggtgg ggtgacagcg ttggtggtgg
     2641 ctcgactgga tccaacggtc gcagggtgcc cggattctct ggcaagaacc cgttcagtgc
     2701 tgagcactgg aacatgaccg agcagtcccg cgtttaccgt gagatggggc gtgagcggtg
     2761 tgagcagctc gccaaggcgg cggggacgac gctcgggggg cagcgtccgg cggcgaagaa
     2821 gtagcgcgcg gggcagtccc tcaagtggcg caagaatggc cgaaaggagg agcacggtga
     2881 agaataggag ggcgacgggc cagctcggcg gcccaggaac agaagcggga cccggcagtt
     2941 agcttgcagg cagccaccgg atcccgactc gagactctct caccaacgta accacttggg
     3001 gtacgcaggt ttgccgggca ggcctctaca acccagtccc gacgggctcg actctcgtgc
     3061 gctccacttt atacccaacc gctagagatc ggcaaggagc cgtttcctcg atacgttcac
     3121 agagatccat ttagatgcgg cgcgaaacgc accgcgataa aggtccatag cgcgccatca
     3181 gcacctatcc ctggggggct gaaactagga caccatccga ctgaccgggg gcaaagggac
     3241 gaactggtcg ccgatcatag cgagtccgct ggcggtgtga gccccattga tgggcatggt
     3301 cggattccca tccagatttc ccgcttgagt ctttctccgc gccgtgcaag actcaatttt
     3361 gcgatacggc acatggtgct gtgatcgccc cgctggcggc gggggctcgg cccgcatggt
     3421 cgatcgtcga gaaaggccgc aacggcggag ccggttacat ggtgatcgga tccggggttt
     3481 ttcctcgctc tcgatcccat gaggcacgca cgctatggcc gcaggtccgc tgacccaaat
     3541 caccgatatg atcgtccttg aagttttcac tccctacgtt cagcaggtga ccgagcaaaa
     3601 gtctcgactg attcagtccg gcgctctatc acgcagcccg attctcgaca atttcctggc
     3661 gggtggcggg ctcacggtca atgttccgag cttcaccgac ctcgacgacg acgacgacaa
     3721 cgtctcgaca gatgacgtcg cggatgtgat tgcggcaacc gctggtgcgg catggcctag
     3781 cccgagtctc gatgccactc cgctcaagat cactgcggat caggagattg cggttcgtct
     3841 ctctcgaaac aattcctggt cgagcgcaga ccttgcggcc gcgctggctg gtgcggaccc
     3901 catgagttct atcgccgacc gcgtcgcgtt ctactggtcg cgcaaactcc agaaggcgta
     3961 cgtcgcgact tggaacggtg tcatcaagga caacactgcg aacgactccg gcgactacac
     4021 gaacgacatc tcaggcggct cgttcgtcga cggcgtcaca aacttctctg cggaggcgtt
     4081 catcgacgcg accgcgactc taggcgactc ctccgacgac ctcgggatca tgatggttca
     4141 ctccatcgtc aaggcgcgga tgaagaagaa caatctgatc gacttcatcc ccgactccga
     4201 aggtcgcgtc aacatcccga cgttccaggg atacatcgtc atcgaggatg acgggatgcc
     4261 cgtgacttcg agcgtgtacg acacctggat cttcggatcc ggcgcgacca tgcttggcac
     4321 cggctctccc aaggtgccga ccgaagccta tcgcgagccg ctcgctggca acggtggtgg
     4381 cgaggaaatc ttgactaatc gcgtcgagtg gtgcatccat ccgatcggtc acgcctacac
     4441 cggaacggcg ccgaatggcg gccctggtaa tggtacgggc gcaaaccagc tcaacgtcgc
     4501 tggatcctgg aaccgtgtgt atcccgagcg gaagcagatc aagttcgctc gcctcgtcac
     4561 tcgtgaagcg taagctgagc ggaaggaggt gacgacatga cgactcaagc attgttcgag
     4621 gcggcgatgg gcggctcgct catccctctc aaggagatcg agttcgctcg aacctctggt
     4681 gatatctgca ccgcaactgc gcacgggctg gcgacgggtg cgggaccgtt caaggtcacg
     4741 actaccaacg ctgacgcgcc gtccggaatc acggttgccg cgcattcaag ccttgcctac
     4801 acgccgtcga ccgatgtcga ggacgagacg gtgacggtcg cgggcaaggt ctacacgtgg
     4861 aagaatgcgc cgtctgccga tggtgaggtg gacgtgaacg cggatgacaa gatcgcggcg
     4921 gagaatctgg ctgcggcgat caacctcgag cccacggtgg gcacgagtta cggtgcggcg
     4981 atgacggcca atccgacggt cttcgcaaac gtcgcgcctg acacggctgt ctgcacggtc
     5041 tacgcgaaga cactcgatgc cacggtgggc gacgcgatcg cggtcgcaga gtctgcgtct
     5101 ggcgcgtggg ctggcgcggg aaccgttctc gccaacggcg cgagcggtac tgactactat
     5161 gtcatcagcc tcaccgatga cacgttttcg ctggcgacaa ccaaggcgct tgccatcgcg
     5221 ggcactgcgg tgacgctcgc ggacgctggc accggcacgc acttcctcgt gatgacggcg
     5281 aaggcgctgg cgaccggcct gaatgacgtg ctgaccaact tcctcaccgc tactggcacg
     5341 cggtgtatcc ctgcggcgga caacgtcgcg aacttctggg caaccgcgat cgatggtgtc
     5401 gcggtgcatc ggagctgacc atgaccaaag gacttccata ttcgatgagt cgtggcgctc
     5461 ccttgcgtca ggagatcatc aagcagaccg tgaccgtgcg cgatgcagcg gtaaccgtgg
     5521 atggggccac tggcgttgga ttcgggtccg tggtgatcgg cgacatcccc gaagggaaca
     5581 tcctcattct gggcgcggtg gcgtacatgc agttcaccgg cccaacctct gcgagtctcg
     5641 atgacgactg ggttggtgac tacggcattg ggtcaacccc tgccgatgat gcgacgatca
     5701 cgacaactga catcaacatc atcggatcca cggcgatcgc tgcggcaact gctgaggcgt
     5761 caccgagaac gcgcggcaca tcgaacacga ccgacctcga tcacatcctc gacaacacgg
     5821 atgggtcact ggagatcaac ctcacgctcc tcgtggatga cgcaaacatc ggcgccgatg
     5881 gcctcgactt cacggtaaat ggcgagctga cgctggccta tatcatgctg ctggacgact
     5941 gagaaatcgg cccaccgtcg cagaaattcg tgccggtggg cctcaacagg agacgacgcg
     6001 acgtgaatct tctgcaagcg ctggagagcc tggacccgct gaattctgag caatggacga
     6061 tgcagggact ccctcgtatc gaggcggtgt ccgagatcat gggtcgtgag gtcacacgca
     6121 tggacatcac caacacggcc ccaagactcg tgcgtgagaa cgcacgtccc aaggcgcagc
     6181 ccgccacggt ggataccgag ggcaacgtga tcggtgaatc cgcagatgct ccggagcgcg
     6241 tcgagattga agatccgaag atgacggcgc atgtcggccc tcatgatgat gtcgtgggga
     6301 tgcccgctgc tcaggtgtac ggcagcgccg agctgatcga tcgtgcgctc gatgagtttt
     6361 cgcggcagtc tgacatcctg accgctcgcc gtgaggcgat cgcggagaag atcaaggaat
     6421 tgagcaagcg gacggagctt ctgaccagag cgcgcaacac actcgcacgt gcgggaaaga
     6481 tcaaggatac ggccaccccg atttcggatt acctcaagtc gcagcagcac gctcgtgcga
     6541 tgcgggcgga gcgtgctcgg cgcttcatcg acgcggggac aaccgagcgg gacgtgattc
     6601 tcgggatgaa gggggtttct cccttggatg cagcgatcgc cgcgaagaac acgaaggcga
     6661 aggcgccgca gcccttgcgc gtctctatgg gccaagggtg atctgtgcca gacactccgc
     6721 agatcagtaa gccgctgtct gatctcgagc aagctgtctt ttttcatgct cgagagcgac
     6781 ggcgctcgct tgctggtgtg ccggagatgt atgggacgca catcattccg attccgacgc
     6841 gcccaatcct tggaaatcgg tttccgtccg ttgatatccc ggcgacgttt tggactgcaa
     6901 tcaggattac cggcgcatct cccgcggggc tcatattcga gcttggcaac gacaccacgg
     6961 gcatcgcggc gtgggttgac gatgacacga ttgggtttcg agccggagat gcggctgctg
     7021 gtgattttgg gtccgcgctc tttgtgaacg cggcggggac gctgccggat gggcttgagc
     7081 ttgatctcgt gtttgcggtg cgccctggcg acgggcgagt tcgggtgtgg ggtaatgggc
     7141 gtgagatcgc gcgaggggtc gcagcgaacg gccagcttcc gaatggttgg tcgagcggca
     7201 cgaacggatc atttgctctg aataggtctg ggaacactcc tccagacgtg acggagtttg
     7261 ggatcccagc agactttgcc tgcattgaac cgttgcgcgc tttcatgggt cagatcccgc
     7321 gccatttcgt gtgatagacc aaggaggttc agccggtggc aagtgtggcg acagcaaggc
     7381 ggaggacgct tctgcgggtc aacgccatcg ctcgtcgcaa gcgcaaggat tcccaccgcg
     7441 gctatcccga cctccaccag acgcacacga ttgccatcgg cacgtcggac gcgatcgcga
     7501 atcacttcga ctctggtgac atggcggggc cggtgacgtt caagttcagg gttgatcgca
     7561 ctagcgactc ggcgaccggg accgtgctgg cgcttggcaa cgagagcacg attggtttcg
     7621 actccggcga cctcgagata ttcactggcg gcggatcggg agacacattc accggcacct
     7681 tcatcggggc gccccgagac tccgccgagg tggtcgtggc gctccatcca tcgacgggac
     7741 aggtgcgcgc ctgggtcgat tctgtgcgcg tgatcgcggg cactgtctcg acttatccat
     7801 ggtcgtgggc tgcgacgggg acgctcgatt acgatgggat ctccggactg tccgtgcgcc
     7861 aggacctcta cgcctacgtc ggtcagatcc cgcgccactt cttcgaggtt tcggtgggcg
     7921 gtgccggatc cggcggggtc gtgttcgatg atctgctgtt tcgagcatcg cggcaggcca
     7981 cggacctcgc gacgtcgaga ttcccgtaca tggagaggcc ggactgatgg gcaacgtcgt
     8041 acacgcacgc agggatgttt ccgcgtcttc ttctggcgtg acggaccatg gtgcgctcac
     8101 cgggctcact gacgatgacc acacccagta cctcctcgaa gacggctcgc gtgcgatgtc
     8161 cggcgacctg gacatgggca ccaacgcgat cacgaacgtc ggcaacgtcg atggggtgga
     8221 cgtgtcagcg cacgctggag acgccacgat ccatttcacg gaaggctcca tcgatcatac
     8281 tgcgatcacc aatatcggga ccaacacaca cgcgcagata gacacgcatg tagccgacag
     8341 cactattcac ttcatagaag gatccatcga tcatacggcg atcgccaaca tcggcacgaa
     8401 tacccacgcg cagctcgata cccacgtggc ggactcaacg atccacttca ctgaggcgtc
     8461 catcgaccat acggcgatca ccaatatagg gacgaacacg cacgctcaac tggataccca
     8521 cgttgccaca tcgaacattc actttaccga ggcatcaatc gatcacaccg ccatcactaa
     8581 tatcggatcg aactcgcacg cggcgatcga cactcatatt gctgacgacg agattcactt
     8641 cacgtatgcg cagaactcgc aaccgtcgaa tccaagcacg gacggtgtga cttggctgcg
     8701 cactgggtct ggcgcggcgg agatatactc ctgggacaac acgaacgggt tctggatttc
     8761 ctttggtgac tggtgctgga tgatctccag cgcggcctcg gacgtgtcag cgtccccgtc
     8821 gatcgctcag tttgttctgg caaagatggt gcagatcacc atgaacgaca ccggcaagtc
     8881 gatccgcgcc ccgttcaacc tccagatgta tcgcttggcg atgaacgctc aggatgttcc
     8941 gctgagttcg gatggcatcg acatcaaggt gaacgccgcc acaacaaccg tgtactggga
     9001 tggcaccaac aactacatcg tgcaggacat caacgccacc gccgacgtga ccaaccgcat
     9061 ccgtttccag tggcgtttcg acagcaccaa accagcggca acgcagcgcc tctcggcgca
     9121 aatctacgtt cggcgaagga ttccggcgct ttgatggggg ctctcgatga caactgatat
     9181 cgttgccttt gtcaccaaca acgacgcgcc tcttacctct ccgactgacg agccgacgat
     9241 caggattcgg aggctcgata ccggcgcgct cgtggtcacg gatcaggaca tgacggagat
     9301 cggagacggc ctctggtcct actccttcga tgagtcgtca ctgctcacct acggagtgag
     9361 agttgacgca gatcccgccg catcaagtca ggtgaccgcg gctgaaaggt actattccg
//
LOCUS       DASRZF010000341         8097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2326186, whole genome shotgun sequence.
ACCESSION   DASRZF010000341 DASRZF010000000
VERSION     DASRZF010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 8097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8097
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2326186"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..828
                     /locus_tag="VFG22_15165"
     CDS             235..828
                     /locus_tag="VFG22_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417636.1"
                     /translation="MLRFRLLPVILGPVLAALLFTSPAHGSIVQGLDLEELVSNADRI
                     VLGRVVLSESFPRPNGQIATWHRIEVKRELRGHAPDETEVIVETLGGQLGDLGMRVEG
                     EPSFEVGERVIVFIRDGGPYTAFRPVGMGQGVMRVRKEQGVDTVTQNRDGLLLMRRGT
                     KGVLERSPGALPHKERLDTLLLKLQDLVARSAGGIDE"
     gene            821..1789
                     /locus_tag="VFG22_15170"
     CDS             821..1789
                     /locus_tag="VFG22_15170"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HET6417637.1"
                     /translation="MNSRRLYAASLGLALMTAFLADHASAYCRMTTQGGAQVGGAACS
                     EKGEPLVWDTACLSYAIDSRGSRWMDSEDVDFAVDGAFASWMDADCGGSPPNLIFQPL
                     QPSTCQRAEFNDTGNVNTIAFLDPWKDPCAESGGYDPFAFAVTVVWHNTSTGEIYDAD
                     MMINDRLATGSNAGGPYAICPDTGCPSGNPGPADLQSIVTHEAGHFIGIGHSDVEEAT
                     MLATAERDSVEKRTLASDDIEAVCDIYPPGNLDSSCNAAPLCGLELDCEAAACSSTTG
                     TGDCGKSGGGCSASGTPSDAPWMTLLAGMLGITVMRRRSARLAARS"
     gene            complement(1743..3782)
                     /locus_tag="VFG22_15175"
     CDS             complement(1743..3782)
                     /locus_tag="VFG22_15175"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET6417638.1"
                     /translation="MRRSWTLLSLGGVFALACVAQVRAQDAAESAASAREQQLVEVQN
                     SFSGPTGGIRIVDAGSGPKGSFRLALNTEFFIISDFFVPTDRSHYFAGNLSLSVTPTE
                     YLEVFASAAVTSAWDDSNDPMLIQRVADVLLGLKGFHRVRPWLTLGGDASVVFPGGVG
                     DAGATFRATSFGFRGNVRFDFRERERRAVPLITRFNAQYWFDNSAELTASIEERRYAA
                     LGGTVPPDLETRQLLTPFERYAYGVNRTDFVRIAAGFEAPLHAGKVGLHPMLEWQWGI
                     PVNRQGFDCAQAASANDDSCLADAGLRAFPMLLTMGLRILSPPKGLAFTVAVDVGLTG
                     TRQLVRELAPTAPYDIILGIAYAFDARSPAPSIPVPVEEVVEAVAITGEVRGRVLDEG
                     SGSPVAGALVAVVGQDASPQATDEDGRFVTFGIPEGEVQLEVSHPEYEPSRCSVSVPS
                     DSDADCRLVPSSLDGQLRLLAIDRNGEPVPAIAITVRGPSEHRLVSDQTGVARVDALK
                     PGAYTTHLDDPVYLIAVADVEILPRQETTVQLRVLPKPSRPGVVIKKTQIALRRQISF
                     ATGSDEILPNSEPILLEVADALLRNPQLELIEIQGHTDSRGDPNLNMRLSQQRAESVQ
                     QWLIRHGVEPARLMAKGYGSTRPIAPNITQQNRAHNRRVQFMIVRRVELNAAASP"
     gene            complement(3848..4156)
                     /locus_tag="VFG22_15180"
     CDS             complement(3848..4156)
                     /locus_tag="VFG22_15180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417639.1"
                     /translation="MRRVAAIVVCFLSLMATSAVKADAVVHVKVRSADNKPVDGRVQL
                     SGDGGTFTCTTSQGSCTLSAVPGGRYVAVFTPKSGSATSPKKVMIPPDGSADLHIAAK
                     "
     gene            complement(4163..5344)
                     /locus_tag="VFG22_15185"
     CDS             complement(4163..5344)
                     /locus_tag="VFG22_15185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417640.1"
                     /translation="MSQVLTVPTHFNDIGELSEGFLDRVERDTLILYGPVAYEDGSEI
                     EFVVLLADGSPALEGAGRVRAAVDGGADRVPETRYDVVVEALVLDGRYEVVFERLVLA
                     RQAVSDRPLASDDEPVTNEMDSADESDAPDVDDVELTAEEVFSLAPGEIEVETIPPEA
                     AAVDTVPPDAFEDEDEPEYEAATVIAAVDAEAMMAEAEAAAESVSVSASAAVSEPAPV
                     SASAPEPVYDSEPQLEAQSETDLDEEAFGNNEDGETRFDSEYAFEHGIAMAPQVGMPV
                     PVPSMAAPPGAPPEPPRLRLAEPPKGLTRPSLALVEESSFTTDGEVSDVHSTGLFAYE
                     DGLPVPARPPLPDPNRQRAKPSVEAHPDYDVEDSEPPNAQDDEYEEIRLSDFAEAAEE
                     G"
     gene            complement(5344..5655)
                     /locus_tag="VFG22_15190"
     CDS             complement(5344..5655)
                     /locus_tag="VFG22_15190"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF002140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HET6417641.1"
                     /translation="MAILMTRGFGCCMLENMGKSKVRGDSSEETPFEEVMDRLQTVVE
                     QLEQGDLPLEQSLAMFEEGVRLSRAGAKRLDEAERRVEELLAAGDELQTSPLEESEAG
                     E"
     gene            5696..6670
                     /locus_tag="VFG22_15195"
     CDS             5696..6670
                     /locus_tag="VFG22_15195"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HET6417642.1"
                     /translation="MGEGGARSPLRAPDLMRETDSVVTPGNHDGVHLGHRALIDAAKR
                     TADAQGWETLAMCFEPHPTAVLAPERAPVLITEMERRVELLKGAGCDDVVVLPFDDEF
                     ARMTPDRFVERVLLDACRAKGVVVGPDFHFGHKRAGNIATLRRWGEAAGFGVQLVEPV
                     MFGSKAVSSTRLRRILSEDGNVEDAALMLTRVHETSGRVVPGDQRGREIGFPTANLEV
                     APVQLPKDGVYAAVARVLDQPDAPLLHGIANLGNRPTFDAGHSVEVHLFGFEGDLYGR
                     RLRLGFVARIRGEAKFGGLEALRSQIDRDCDQAKNALQQLSPDLVRWI"
     gene            6661..7302
                     /locus_tag="VFG22_15200"
     CDS             6661..7302
                     /locus_tag="VFG22_15200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417643.1"
                     /translation="MDLTRLYNLTTPDLRQEAERLGVSDTYAMSRGQLIHAIRSRAQG
                     PEPEGLLGKVLGFAKWAIQAAQEEPRKEEAKTFRKPSEVPMPSIGSRAESVPASESAA
                     VPESAAAAAAEPAAVPEPVPAAESEPAAVHESGPPSRPPAGVFARSSGMFEEPFPTRT
                     MARILAEQGHFKRALAIYGMLLREAPQDGELRAEADEARARSRARRSETHERP"
     gene            7348..7707
                     /locus_tag="VFG22_15205"
     CDS             7348..7707
                     /locus_tag="VFG22_15205"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6417644.1"
                     /translation="MTQGQTQKRQNDRFPVRISCELTISGQTHHAETKNVSLGGAAIL
                     FQRALSVSEVVTVGFFLTEDGIEDPDRTPFECAASVRWTKPAGPSLHEAGLQFLSPSA
                     DQRANLALFLQRAQPIG"
     gene            complement(7704..>8097)
                     /locus_tag="VFG22_15210"
     CDS             complement(7704..>8097)
                     /locus_tag="VFG22_15210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417645.1"
                     /translation="SFLAFGAYLHALGMISPRASLTASLYSSAAAVLGAILFLGGWPL
                     VGSVAGIAVLVAKSGAIMLASLGFSMNPKTASACAALALGLAALGLIVDFGALFPHWS
                     ALAAGSVCAIVLRALAPPLRRESAPALV"
BASE COUNT         1534 a         2614 c         2550 g         1399 t
ORIGIN      
        1 cgcgcgagtg aacagccccg ccgtgctgtc ggcggtaaac ttcgcgacgt cgcaagcgcc
       61 gagcgccgag acacaaacaa gaacgcaaaa caggccagcc gccgatcgca gacggcggga
      121 catcagatcc tggtacatag agcgaggtgt acgaaattta gagggaaatt gtcaatcgag
      181 atcgtgtggg gtcgatcgct tgacggcgcc gagcgcggcc cgcagcatta ggaaatgctt
      241 agattcaggt tgctgcccgt gatcctcggg ccagttcttg ccgctctact gttcacgagc
      301 ccagcgcatg ggtccattgt ccaaggcctg gacctcgagg aattggtctc caacgccgac
      361 cggatcgtgc tcggccgtgt ggttctttcc gagtcgttcc cgcgccccaa cggccaaatc
      421 gccacctggc accggatcga ggtcaagcgc gagctccgcg gtcacgcccc cgacgaaacc
      481 gaggtgatcg tcgaaaccct cggaggccag ctcggcgacc tcgggatgcg ggtcgaagga
      541 gagccctcct tcgaggttgg cgagcgtgtc atcgtcttca ttcgcgacgg cggcccatac
      601 acggcgttcc ggccggtcgg catgggacag ggcgtcatgc gggtgcgcaa ggagcaaggg
      661 gtggacacgg tcacccagaa tcgcgacggg ctgctgctca tgaggcgcgg caccaaggga
      721 gtgttggaac gcagtcccgg cgcattgccc cacaaagagc gtctcgacac cttgctcctg
      781 aagcttcaag acctggtcgc ccgcagcgcg gggggcatcg atgaatagcc gccgcttgta
      841 tgccgcgtcc ctcgggctcg cactcatgac cgctttcctc gccgaccacg cgtcggccta
      901 ctgccggatg acgacccagg gcggagcaca ggtcggcggg gcagcctgtt cagagaaagg
      961 cgagcccttg gtttgggaca ccgcttgtct gtcctatgcg atcgattcga gaggctcgcg
     1021 ctggatggac agcgaagacg tcgactttgc cgtggatggc gcattcgcga gctggatgga
     1081 tgccgactgc ggaggctcgc cgccgaacct gatcttccaa ccgctgcaac cttccacctg
     1141 tcagcgtgca gagttcaacg acaccggcaa cgtcaacacc atcgccttcc tcgacccctg
     1201 gaaggacccc tgcgccgaga gcggcggcta cgatcccttc gcgttcgccg tcaccgtggt
     1261 ttggcacaac acatccacag gcgagatcta cgacgcggac atgatgatca acgaccggct
     1321 cgccacgggg tccaacgccg gcggccccta cgcgatctgc cctgacacgg gttgcccttc
     1381 tgggaaccct ggaccggcag acttgcagag catcgtcacg cacgaagccg gacacttcat
     1441 tggtatcggc cactcggacg tcgaagaagc caccatgttg gccaccgccg agcgcgactc
     1501 ggtcgagaag cgcactctgg cctccgacga catcgaggct gtctgcgaca tctacccgcc
     1561 gggcaatctc gattcgtcct gcaacgctgc gccactctgc ggactagaac tggactgcga
     1621 ggctgccgcg tgctcgagca ccacgggcac tggcgactgc ggtaagagcg gaggcggctg
     1681 cagcgcatca gggacgccgt ccgacgcacc ctggatgacg cttctcgctg gcatgctcgg
     1741 catcacggtg atgcggcggc gttcagctcg actcgccgca cgatcatgaa ttgaacccgg
     1801 cgattgtgag cccggttctg ctgcgtgatg ttcggcgcga tcggacgcgt ggagccgtat
     1861 cccttcgcca tgagccgcgc gggctccacg ccgtggcgga tcagccactg ctgcaccgac
     1921 tctgcgcgct gctgcgagag ccgcatgttg aggttcgggt cgccgcggct gtcggtgtgg
     1981 ccctggatct cgatcaactc gagctgtgga tttcgtagga gagcgtccgc aacctcgagc
     2041 aagatcggct cgctattcgg taggatctcg tcgctgccag tcgcaaagct gatctgcctg
     2101 cgaagtgcga tctgcgtctt cttgatgacg acaccgggtc gcgaaggctt gggcagcaca
     2161 cgaagctgta cggtggtctc ctgacgcggg aggatttcca cgtcggccac ggcgatcaga
     2221 tagaccggat cgtccaagtg cgtcgtgtag gcgccaggtt tcagcgcatc gacccgcgca
     2281 acgccagtct gatctgagac cagccgatgc tcggacgggc ccctaaccgt gatcgcgatc
     2341 gctggaaccg gttcgccatt gcgatcgatg gccagcaatc gaagctgccc atcgagactc
     2401 gacggcacga gcctacagtc cgcgtcggag tccgacggaa ccgagacgct gcatcggctg
     2461 ggttcatact ctggatgaga aacctcgagc tgaacctctc cttctgggat tccgaacgtg
     2521 acgaatcgcc catcttcgtc cgtggcctgc ggggacgcat cctgaccgac caccgccacg
     2581 agcgcgcccg caaccggaga gccgctgccc tcatcgagca cccggcctcg cacctcaccc
     2641 gtgatcgcca cagcttcgac gacctcctcg acaggaacgg ggatcgaggg tgccggagac
     2701 cttgcgtcga acgcataggc aatgcccaga atgatgtcgt aaggcgccgt gggcgctagc
     2761 tcacggacca actggcgggt cccggtaagc cccacatcga ccgccaccgt gaaggccagc
     2821 cccttgggtg ggctgagaat cctgaggccc atggtgagca gcatcgggaa tgcccggaga
     2881 cccgcgtcgg caaggcacga gtcgtcgttc gcactggcgg cttgggcgca gtcgaagccc
     2941 tggcgattga ccggaatgcc ccactgccat tcgagcatcg gatgcaatcc aaccttccca
     3001 gcatgcagcg gtgcctcaaa gccggcggcg attcgaacga agtcggtgcg gttcacgccg
     3061 tacgcatagc gctcgaaggg ggtcagaagc tgacgcgtct cgaggtcagg agggaccgtg
     3121 cctccgagcg cagcgtaccg gcgctcctcg atcgatgcgg tgagctcagc cgaattgtcg
     3181 aaccagtact gagcgttgaa gcgcgtgatc aagggcacag cgcgacgctc acgctctcgg
     3241 aagtcgaatc ggacgtttcc tcgaaagccg aagctggtgg cgcgaaacgt ggcgccggca
     3301 tcgcccaccc cgcccgggaa aactacgctt gcgtccccgc caagggtcaa ccagggcctc
     3361 acccgatgga atcctttgag gcccagcagc acatcggcga cacgctggat caacatcgga
     3421 tcattggagt catcccaggc agacgtgacc gccgcagaag cgaagacctc caggtactcc
     3481 gtcggcgtca cggagagcga aaggttgccc gcgaagtagt gcgaccgatc ggtcggcacg
     3541 aagaagtcgc tgatgatgaa gaactcggtg ttcaaggcga gccggaagct tcccttgggc
     3601 cccgatcccg catcgacgat gcggatgcca ccagtggggc cgctgaagct gttctgaacc
     3661 tcgacgagct gttgctcgcg cgcgctagca gccgactcgg cggcgtcttg cgcacgaacc
     3721 tgtgcgacgc atgccaatgc gaaaacgccc cccagtgaga gcagtgtcca gctcctgcgc
     3781 attcccctgc ctgtccccca cccaaaatga tgccatttca gcggtgcctc acgccaactt
     3841 gacgggccta tttcgcagcg atgtgaagat ccgcgctgcc atccggcggg atcatcacct
     3901 tcttgggaga ggtcgcgctg ccgctcttcg gggtaaatac cgcgacgtat cgaccgccgg
     3961 ggaccgcgct cagcgtgcag ctcccctgac tggttgtaca ggtgaaggtg ccaccgtctc
     4021 cgctcagctg tacgcgccca tcgacaggct tattgtccgc gctgcggacc ttgacgtgaa
     4081 ccacggcgtc ggccttcacc gcgctcgtcg ccatcaggct caggaagcaa acgacaatcg
     4141 cggcgacgcg tctcatctgg gctcatccct cttctgcggc ctcggcgaaa tcgctgaggc
     4201 gaatttcttc gtactcgtcg tcctgcgcat tcggtggctc ggagtcttcg acgtcgtagt
     4261 ccggatgtgc ttcaacgctt ggcttggccc gttgccggtt cgggtccggg agcggcggtc
     4321 tagctggcac gggcaaaccg tcttcatagg cgaaaagacc agtcgaatgc acgtcgctga
     4381 cctctccgtc cgtggtgaac gaagattctt cgaccaaggc gagagaaggc ctcgtaaggc
     4441 ccttcggggg ctcggcaagt cgcaacctcg gcggctcggg cggcgcccca gggggagcgg
     4501 ccatcgaagg aactggcacc ggcattccaa cttgcggtgc catggcaata ccgtgctcaa
     4561 atgcgtactc ggaatcgaag cgggtctcgc catcctcgtt gttgccaaag gcttcttcgt
     4621 ccagatccgt ctccgactga gcctcgagct gcggctccga atcgtacact ggctctggcg
     4681 ctgacgccga cactggcgct ggctctgaca ctgccgctga cgccgacact gacactgact
     4741 ctgccgctgc ttccgcctcg gccatcatcg cttcggcatc caccgccgca atgaccgtag
     4801 cagcctcgta ttcaggctca tcctcgtcct cgaacgcgtc gggcggaacg gtgtcgaccg
     4861 ccgcggcttc gggaggaatg gtctccactt cgatctcgcc gggtgctaag gaaaacacct
     4921 cctccgcggt gagctcgacg tcgtcgacat caggcgcgtc gctttcgtcc gctgagtcca
     4981 tttcgttcgt gaccggctcg tcgtctgaag cgagaggtcg atcagacacc gcttgtcgcg
     5041 cgagcacgag ccgctcgaag acgacttcgt aacggccgtc gaggaccaaa gcttcgacga
     5101 cgacgtcgta gcgtgtctcg ggaacccgat cggccccgcc atcgacggcg gcacgtacgc
     5161 gacccgcccc ttcgagggca ggagagccgt cggcgagcag gaccacgaat tcgatctcgg
     5221 agccgtcttc gtaggcgacg ggcccgtaga gaatgagtgt gtcgcgttcc acacgatcga
     5281 gaaagccctc ggagagctct cctatgtcgt tgaagtgtgt tggcaccgtg aggacttggc
     5341 tcattattcc cccgcttcgc tttcttcgag agggctcgtc tgcagctcgt ctccggctgc
     5401 aaggagctct tctactctac gctcggcttc atccagtctt ttcgctccgg cccgcgatag
     5461 gcgaaccccc tcctcaaaca tcgccagaga ctgctcgaga ggcaaatctc cttgctctaa
     5521 ttgttcaacg accgtctgga ggcggtccat gacctcctcg aatggagtct cctcagagga
     5581 atcgccccgc acctttgatt tccccatgtt ttcaagcata cagcacccaa agccccgcgt
     5641 catcaagatg gccatggaat gcattcgagt tgaagtgttc ccgacggcgc tgtaggtggg
     5701 tgaaggagga gcgcgttcac cgctccgggc cccggacctg atgcgcgaaa ccgattctgt
     5761 cgtcacccct ggcaaccacg atggcgtgca cctgggtcac agggcgttga tcgacgccgc
     5821 caagcgaacg gcggacgccc agggctggga aacgcttgcc atgtgcttcg agccccaccc
     5881 cacagccgta ttggcgcctg agcgggcgcc cgtcctgatc accgaaatgg agcgtcgcgt
     5941 cgagcttctc aagggggcgg gctgcgacga cgtcgtggtc ttgcccttcg acgacgagtt
     6001 cgcccgcatg acacctgatc ggttcgtcga gcgggtcttg ctggacgcct gccgggccaa
     6061 aggcgtcgtc gtcgggcccg acttccattt cgggcacaag cgcgccggga acatcgcgac
     6121 gctgcgtcga tggggcgagg cagcaggttt cggcgtccag ctcgtggaac ccgtgatgtt
     6181 cggtagcaag gcggtttctt cgacgcgcct gcggcggatc ttgagcgaag acggaaacgt
     6241 ggaagatgcg gcgctcatgt tgacccgggt tcacgagacc agcggtcgtg tcgttcctgg
     6301 cgatcagcgg ggccgagaaa tcggatttcc gacggcaaac ctcgaagtgg cgcccgtgca
     6361 gctcccaaag gacggcgtct acgcggcggt agcccgggtg ctcgaccagc cggacgcccc
     6421 attgcttcac ggaatcgcaa acctgggcaa ccgtcctacg ttcgatgccg ggcattccgt
     6481 cgaggtgcac ctcttcggct tcgagggaga tctgtacgga aggcgcctac gattgggctt
     6541 cgtcgcacgc atccgcggtg aagcgaagtt cggcggtttg gaggcccttc gttcacagat
     6601 cgatcgagac tgcgatcagg ccaagaacgc gctacaacag ctgagcccgg acctggttcg
     6661 atggatctga ctcgacttta caatctcacg acccccgatc tccgccaaga agccgagcgc
     6721 ctgggcgtga gcgacacgta cgcgatgagc cgcggccaac tcattcacgc gattcgaagt
     6781 agagcccagg ggccagagcc cgaaggcttg ctcggaaagg tcctcggctt cgcaaagtgg
     6841 gcgattcaag ccgcccagga agagccccgg aaagaggaag cgaagacctt ccgaaagcca
     6901 tcggaagtcc caatgccctc gatcgggtca agagcagagt cagtgccggc gtcagagtca
     6961 gcggcagtgc cagagtcagc ggcagcggca gcggcagagc cagcggcagt gccagagcca
     7021 gtgccagcgg cagagtcaga gccagcggcg gtgcatgagt ccggccctcc gagccgccct
     7081 ccggctgggg tctttgctag aagctccggg atgttcgaag agccgtttcc gacgcgcacg
     7141 atggcgcgga tcctcgccga gcaaggccac ttcaagcgcg cgctggccat ctacgggatg
     7201 ctcctccgcg aagcccctca ggacggcgag ctgagagccg aagccgacga agcgcgagcc
     7261 cgttcgagag cccggcgatc agagactcat gagcgcccgt agcaccggca attttgatcc
     7321 gggccgcatc tttcgcatca tgggaatgtg acgcagggac agacgcagaa gcggcagaac
     7381 gatcggtttc cggttcgcat ctcttgcgag ctcacgatct cgggccagac gcatcacgcc
     7441 gaaacgaaaa acgtcagctt gggcggcgct gcgatcctct ttcaacgcgc cctcagcgtg
     7501 agtgaagtcg tgactgttgg cttcttcctc accgaggacg gtatcgagga cccggaccgc
     7561 acgccattcg agtgcgctgc gtccgtcagg tggaccaagc cagcggggcc gtctctccat
     7621 gaagccggac tgcagtttct gtcgccctca gcagatcagc gggcaaacct cgcgctcttc
     7681 ctccaacgcg cgcagccgat cggctagacc agcgcaggcg cggattcgcg tcggagcggc
     7741 ggcgccaggg cgcgcaggac gatcgcgcaa accgacccgg cagcgagcgc gctccagtgc
     7801 ggaaagagag cgccgaagtc cacgatgagc ccgagcgctg ccaggccgag cgcgagcgct
     7861 gcgcaagccg aagctgtctt cggattcatg gaaaatccca gcgacgcgag catgatggcc
     7921 ccggacttgg caacgaggac agcgatgcct gcgacggagc cgaccagcgg ccagcccccg
     7981 aggaacaaga tcgcaccgag gacggcggcg gcggacgaat agagcgacgc cgtcagcgag
     8041 gcacgggggc tgatcatccc gagcgcgtga agataagctc cgaaggcgag gaacgat
//
LOCUS       DASRZF010000342         2081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4730229, whole genome shotgun sequence.
ACCESSION   DASRZF010000342 DASRZF010000000
VERSION     DASRZF010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4730229"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="VFG22_15215"
     CDS             <1..634
                     /locus_tag="VFG22_15215"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HET6417646.1"
                     /translation="DQLEATLEALRPHVKLVDLSLPFDLAQIVPLYVRLLLGVIGADM
                     PGPLKALARVLLPYYALADRLGIETDLVIKNAVRGIHLSHADWNRANEGRTRLRWQCH
                     ALFQEIDVLLTPIGPVTAFPHQTGGNHLSRRLRVNGQSRPYMDHLCWVALASTAFLPA
                     TSAPVGFTEEGLPVGIQILGPYLGDRTTIRFAELLSEVRGGFIPPSLTPR"
     gene            673..1914
                     /locus_tag="VFG22_15220"
     CDS             673..1914
                     /locus_tag="VFG22_15220"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HET6417647.1"
                     /translation="MTTLPRKRDRSRELDIVLWGATGFTGRLVAEYLVKNYIGGETGL
                     RLALAGRNGEKLEAVAREIGAPELPILLGDSFDVEALDALASRTEVLISTVGPYAKYG
                     AELVGACVRHGTDYCDLTGETQFVRAMIDVHHAEARKTGARIVHCCGYDSIPSDLGTL
                     MVQEEFKKRHGRYAGEVKMAAVDMRGAFSGGTIATMMNIVDEIKENPSIRKILGNPYA
                     LNPKGVRGPDKGDQTGARFDKDFDMWTGPFIMAAINTRIVRRSHALMGEPWGSEFRYS
                     EVMGTGQGPKGFARAASIAAGMVAFMASLAIPLTRPFVERRLPSPGEGPDAEARAKGR
                     FKTLLLALGDARVVRGTVADDRDPGYGSTAVMISESALCLALQGSELASEGGILTPAT
                     AMGMKLVERLRAAGMTFEVAV"
BASE COUNT          379 a          691 c          637 g          374 t
ORIGIN      
        1 cgatcagctc gaagcgacgc tcgaagctct gcgtccgcac gtaaagctcg tggacctcag
       61 cctgccgttc gacttggcgc agatcgtgcc gttgtacgtg cgcttgctcc taggcgtcat
      121 cggtgcggac atgccagggc ccctgaaggc gctcgcgcgc gtcttgcttc cgtactacgc
      181 gctcgccgac cgccttggca tcgagaccga cctcgtcatc aagaatgccg tccgggggat
      241 tcacctctcc cacgctgatt ggaaccgtgc caacgaagga cgcacgcggc tccgatggca
      301 gtgtcacgcg ctcttccagg agatcgacgt tctgctcacg cccatcggcc cggtcactgc
      361 atttccacat cagacgggcg gcaatcacct ctcgcgccgg ctccgcgtaa acggccagag
      421 ccgaccctac atggaccatc tttgctgggt cgctctcgcc agcaccgcgt ttttaccggc
      481 gacgagcgca ccggtgggct tcacagagga aggcctccct gtgggcatcc agatcctggg
      541 tccgtacctc ggggaccgaa ccaccattcg cttcgcagag ctgctctcgg aggtgcgagg
      601 cgggttcata cctccaagcc ttaccccgcg ctgacgctgg cgctgtccct cagactgccc
      661 tatgtctcca agatgacgac actgcctcga aaacgcgacc ggtcccgtga gctcgacatc
      721 gtactctggg gcgcaaccgg cttcaccgga cgcctggtcg ccgagtacct ggtcaagaac
      781 tacatcggcg gcgagacagg ccttcgactc gcgctcgcgg gtcgcaacgg ggagaagctc
      841 gaagcggtcg cgagagagat cggcgccccc gagctcccca ttctgcttgg cgatagcttc
      901 gacgtcgaag cgctcgatgc acttgcatcc cgaaccgaag tgctcatcag caccgtcggc
      961 ccctacgcga agtacggcgc cgagctcgta ggcgcctgcg tgcgtcacgg aaccgactac
     1021 tgcgacctca ccggcgagac ccagttcgtg cgcgcgatga tcgatgtaca ccacgctgaa
     1081 gcgcggaaga cgggtgctcg catcgtgcac tgttgcggct acgactcgat tccctcggac
     1141 ctcggcacac tgatggttca ggaagagttc aagaagcggc acggacgata cgcaggcgag
     1201 gtcaagatgg cggccgtgga catgaggggc gccttcagcg gcggcaccat cgcaaccatg
     1261 atgaacatag tcgacgagat taaggagaac ccctcgattc gaaagatcct cggcaacccg
     1321 tacgcgctca atccaaaagg cgtccgtggt cctgacaagg gcgatcagac gggcgcacgc
     1381 ttcgataagg acttcgatat gtggaccggc cctttcatca tggccgccat caacacgcgc
     1441 atcgtgcggc gcagccatgc gctgatgggc gagccctggg gctccgagtt tcgctactcc
     1501 gaagtgatgg gcaccggcca aggcccgaag ggctttgcac gagccgcgtc catcgctgcc
     1561 ggcatggtgg cgttcatggc gtcgcttgcg attccactca cgcgtccatt tgtcgaaagg
     1621 cggctcccct cgccgggcga aggaccggac gcagaggcgc gggccaaggg ccgattcaag
     1681 accctgctcc tggccctcgg cgacgcacgc gtggttcgcg gcaccgtcgc agacgatcgc
     1741 gaccctggat acggctcgac ggccgtgatg atctccgagt ccgccctctg tcttgcgctg
     1801 cagggcagcg agctagcctc cgaaggcggc attctcacac cggcgacggc catggggatg
     1861 aagctcgtcg aacgccttcg cgccgcaggg atgaccttcg aggtcgcggt ctagcagctt
     1921 gctcggaatg gtcgcggggg ctctgaacac cggtcgcgct tgcgtgcgcc cccacaatgg
     1981 ccgtatggat cagccgctag gagcaaatcg atgcgaaaac taggtgtagc gcttgcgttt
     2041 ctggtgactc tgctcttcgc gggtaccctc tactacttca t
//
LOCUS       DASRZF010000343         6366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1291867, whole genome shotgun sequence.
ACCESSION   DASRZF010000343 DASRZF010000000
VERSION     DASRZF010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 6366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6366
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291867"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="VFG22_15225"
     CDS             <1..752
                     /locus_tag="VFG22_15225"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HET6417648.1"
                     /translation="ENYARLQVDDTSAVRVIVKAGDDAVTELMIGKYGNGMTMVRVGD
                     RDEVFGASGSLRYAFDRELKGWRDRKIVDVAADGVQSIRFESPNGTFQFQRGEQGWTI
                     VEGRKALDEFDPKQLDALVTTAARLIASDFAADEISPARAGLTEPGATVTLTVAEDPN
                     PIVLDLGDSTAESGEVYLRRQGNETLYLVSTYLADRLQPGATAFKKPEAPAAPPPGMP
                     SPVPGGEQQPQLPPEVMRQLQEQIRAQQQQR"
     gene            complement(749..1144)
                     /locus_tag="VFG22_15230"
     CDS             complement(749..1144)
                     /locus_tag="VFG22_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HET6417649.1"
                     /translation="MSRIFVILSGLLGFAAVALGAFGAHGLRSRLDALPDGVKRLDWW
                     NTAAHYQLSHALALAVAAWLVHRGAGTSALVAGWSFVGGVALFSGSLYLMTITGQTKL
                     GAITPFGGLLFLIGWGSIAVAGWRLGAAD"
     gene            1258..4197
                     /locus_tag="VFG22_15235"
     CDS             1258..4197
                     /locus_tag="VFG22_15235"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HET6417650.1"
                     /translation="MISEFGVNQGLVEEQFLKWVDNPLAVDEAWRTYFEGLAPNELPQ
                     ISSAGTVLAPITSVGHLSPAPLPREGNGAAARGPASPSELEYEEGVRNSVFLPPTAET
                     TLAPIISEGRTSFPPSAEVLAANELQARLSALINAYRVRGHLFANLDPLGLHRAPPDD
                     ELLLKRYGLAHVDPETVFSTGDLAGPPTEPLRAVLERLKDTYARTIGVEFTFLENQEA
                     RDWLRDRMELTGNHIDLSREQQIRILTKLTDAEIFEQFLHTKYTGAKRFSLEGGESVI
                     PMLDILTERASELGVEEIVIGMAHRGRLNVMVNIMEMNVRQIFAGFEDDDPEKYVGRG
                     DVKYHLGYSLDRKTASGNDIHMTLAFNPSHLEWVNPVVEGRVRAKQDRKNDVERISVL
                     PLLIHGDAAFVGQGVVAETLNLSRLQAYETGGTVHVVINNQIGFTTNPEDSRSSSYCT
                     DITRMLRCPVFHVNGEDPEAVAQAVTLSAEYRQRFHADVVLDLFCYRKYGHNEGDEPR
                     FTQPEMYAAIDRKQTVREVYIEHLVKTGKLTVEQAEEIKIRRQQDLENALEDTRKNGN
                     YNLIPASMLGLWTEYRGGSDLDVPDADTRFPRERLDNALDRLTTYPDWFTPHPRIERF
                     VLDKQRKVMETGEGVDWGTAENLAFASLLLEGVRCRLTGQDVRRGTFSHRHAVIFDSE
                     TGRRYTRLGHLSPDQAQLEIYDSSLSEAGVVGFEWGYSLDSPDALVCWEAQFGDFVNT
                     AQVIIDQFISSSEDKWHRLSGLVMLLPHGMEGQGPEHSSARLERFLLLCAEDNMQVVN
                     LTTPAQLFHCLRRQVLRPWRKPLIVMSPKSLLRHRRAISTMDELAEGAFQRIIPDDSA
                     IDPKKVRRLVMCTGKIYYDLLERRERDEVDDIGIIRVEQLYPLRASELQKRLAPYRKD
                     VDLVWVQEEPWNMGAWYFMRARLPEILGNEQPLRCIARPESASPATGSGASHKLEQKL
                     LVDEVFS"
     gene            complement(4255..4668)
                     /locus_tag="VFG22_15240"
     CDS             complement(4255..4668)
                     /locus_tag="VFG22_15240"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET6417651.1"
                     /translation="MLVRWDPFEEMNRLHDHFFSGKGLAKQAFQVAVDIREEDDAFFV
                     DAEVPGLTADDVNIDVEKNVLTIRGERKVEKEETEGTYRRVERQYGSFTRSFTLPETV
                     DADSISADLKDGVLALRLPKKEAPTPRSISVNASK"
     gene            4840..5364
                     /locus_tag="VFG22_15245"
     CDS             4840..5364
                     /locus_tag="VFG22_15245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417652.1"
                     /translation="MRDLRLVFVLALTTGVVSLGCVEDVRFNPSGCDVHVRGRWRVDG
                     QDPTPEVCSNIGLVELVIIDEPELQFWSPPEFLLRCEAADDSNAVVIDGGAYLDTLIV
                     TRNRCDGTGAVLKDTLDGQYKSRWRAITDLKFIVDCSPIEVNTITPIDGGTQLLLDVG
                     TVEFQTRDAGPICP"
     gene            5410..6105
                     /locus_tag="VFG22_15250"
     CDS             5410..6105
                     /locus_tag="VFG22_15250"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET6417653.1"
                     /translation="MFRFPPLTPFVKKLLIALFGAWILQILLQNWAGVPIFEVLALNT
                     ARLGVATLWQLGTHVLAFPTGPQAMFSMLFSLLFLWLMLAPFEQRFGQKRTIQLCVVA
                     VLASAALALMVGLLVPSPRALYGAQAILLGSIAAFAWSYRGQGRMSFFGVIDMKPVHI
                     IYLVLALSALMFMLSGDAVALAADVGAIGGGMGFIEWLRRPPRRRRKKKRKKKGSLDL
                     VQGGRNDKPEWLN"
     gene            6107..>6366
                     /locus_tag="VFG22_15255"
     CDS             6107..>6366
                     /locus_tag="VFG22_15255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417654.1"
                     /translation="MLVRGLILAILGTSISAGATGCADESTVIIPVYDACFTVDECVE
                     TATLCEELAVDFAGFTYSNSICTLTCGAQGSVSPDCPIAWVGR"
BASE COUNT         1265 a         1966 c         1922 g         1213 t
ORIGIN      
        1 ccgagaacta tgcccgcttg caagtcgacg acacgagcgc cgtccgtgtg atcgtcaagg
       61 ccggtgacga cgccgtcacc gagctcatga tcggcaagta tggcaatggc atgacgatgg
      121 tgcgggtcgg cgatcgggac gaggtcttcg gcgcaagtgg ctcgctccgc tacgcattcg
      181 atcgggagct caaaggctgg cgcgaccgta agatcgtcga cgtagcggcc gatggcgtgc
      241 agtcgattcg gttcgaaagc cccaacggca ccttccagtt ccaacggggc gagcagggct
      301 ggaccatcgt cgaaggccga aaagcgttgg acgaattcga tccgaagcag ctcgatgcgc
      361 tggtcacgac cgcagcgcgg ctgatcgcgt ccgatttcgc ggcggatgag atctccccag
      421 cgagagccgg gctcacggag cccggggcga cggtaacctt gactgtcgcc gaggatccga
      481 atccgatcgt gctcgacttg ggcgatagca ccgctgaatc aggagaggtg tatcttcgac
      541 gccagggcaa cgagaccctc tatctcgttt cgacctacct cgccgaccga cttcagcccg
      601 gtgcgaccgc gttcaagaag cccgaagccc cggcggcccc gccgcccgga atgccaagcc
      661 cggtgccggg aggggagcag cagccccagc ttccacccga ggtcatgcga caactccaag
      721 agcagattcg ggctcagcaa caacagcgct agtcagccgc gcctagtcgc catcctgcaa
      781 cagctatcga tccccaaccg atcaagaaga gcaggccgcc gaagggagtg atcgccccga
      841 gcttcgtctg cccggtgatc gtcatgaggt agaggctgcc gctgaaaagc gcaacgcctc
      901 ctacgaacga ccagccggcg accagagccg aggtccccgc accccgatga accagccacg
      961 ctgcgaccgc cagcgctagc gcatgactca gctgatagtg cgcagcggtg ttccaccagt
     1021 ccaaacgctt cacgccgtct ggaagcgcgt cgagacgtga tcgcagccca tgcgcgccaa
     1081 acgcccccag ggccacggca gcaaaaccga gcaagcctga tagaatgacg aagatcctgg
     1141 acattgcccc aatcatgggc caactcactc tgcgtaacca gggcgctcgc tagcacgtcg
     1201 gaatcggacg ggttaggctt ggccggcgac cggggtgttt ggagccacga ctcggagatg
     1261 atctcagaat tcggtgtaaa ccaaggactg gtcgaggaac agtttctcaa gtgggtcgac
     1321 aaccctcttg cggtcgatga agcctggcgc acgtactttg aaggcctcgc tccgaacgag
     1381 ctacctcaga tctcgagcgc agggacggtg ctcgcgccga tcaccagtgt cggccacttg
     1441 agccccgctc cgctcccgcg ggagggcaac ggggctgctg caaggggccc ggcgtcgcct
     1501 tcggagctcg agtacgagga aggcgtgcgc aactcggtgt tcttgccacc gacggcggag
     1561 accacgttgg ctcccatcat cagcgaaggg cgcacttcgt tcccgccatc tgccgaggtc
     1621 ctcgcagcca atgagcttca agcgcgcctc tctgcgctga tcaacgcgta ccgcgtgcgc
     1681 gggcatctgt ttgcgaacct cgatccattg ggtctgcacc gcgcaccacc cgacgatgag
     1741 ttgctcctca agagatacgg tctggcacac gtcgacccgg agacagtctt ttcgacgggt
     1801 gacttggcag gtccgccgac cgaacccctt cgcgcggtgc tcgagcgctt gaaggacacc
     1861 tacgcgcgaa cgatcggcgt cgagttcacg ttcctcgaga accaggaagc acgggattgg
     1921 cttcgtgacc gcatggagct caccggaaac cacatcgacc tcagccgtga gcagcagatt
     1981 cgcattctca ccaagctcac cgatgccgag atattcgagc agttcctgca cacgaaatat
     2041 acgggcgcaa agcgtttctc tctcgaaggt ggtgagagcg tgattccgat gctcgatatt
     2101 ctcaccgaac gcgcatcaga gctgggggtc gaggagatcg tcatcggcat ggcgcaccga
     2161 ggacgtctca acgtgatggt caacatcatg gagatgaacg tccgccagat cttcgcgggt
     2221 ttcgaggacg acgacccaga gaagtacgta ggacggggtg acgtcaaata ccacctcggc
     2281 tattcactcg accgcaagac cgcaagcggc aacgacatcc acatgacctt ggcgttcaac
     2341 ccgagtcatc tcgagtgggt caaccccgta gtcgaaggtc gcgttcgcgc taagcaagac
     2401 cgcaagaacg acgtcgagcg gatttcagtg ctaccactgt tgattcacgg tgatgccgct
     2461 ttcgtcgggc aaggcgtcgt tgccgagacg ctgaacctca gtcgcctcca agcttacgaa
     2521 acgggtggca ccgtccacgt ggtcatcaac aaccagatcg gcttcacgac caaccccgag
     2581 gactcccgct cgagctcata ttgcacggac atcacacgga tgttgcgctg tccggtcttc
     2641 cacgtgaacg gagaggatcc agaggcggtg gcccaagcgg tgacgctctc tgcagagtat
     2701 cgtcaacgct tccacgcaga cgtcgtgctc gacctgtttt gttatcggaa gtacgggcat
     2761 aacgagggag acgagccccg attcacgcag ccggaaatgt atgcggccat cgaccgaaag
     2821 cagacggtgc gcgaggtgta catcgagcat ctggtcaaga ccggtaagct caccgtcgaa
     2881 caagcagaag agatcaagat ccgtcgccag caagacctcg agaacgcttt agaagacact
     2941 cggaagaacg gaaactacaa tctcatccca gcttcgatgc tgggactctg gaccgagtat
     3001 cggggcggca gcgacctgga tgtccccgac gccgatacgc gcttcccccg tgaacggctc
     3061 gacaacgcgc tcgaccgcct gacgacctac cctgactggt tcacgcccca tcctcgaatc
     3121 gagcgcttcg ttctcgacaa gcagcgcaag gtcatggaga cgggtgaagg cgtcgattgg
     3181 ggtacggcgg agaacctcgc ttttgcaagc cttctgctcg aaggcgtccg gtgccgcttg
     3241 actggccaag atgttcgccg cggcactttc agccatcgcc acgcagtgat cttcgactcg
     3301 gaaaccggcc gtcgatacac gcggctcggt catctgagcc cggaccaggc gcagctcgag
     3361 atctacgaca gctcgctttc cgaggcgggc gtggtgggtt tcgaatgggg ctattcgctg
     3421 gattcgcccg acgctctcgt ctgctgggaa gcgcagttcg gtgacttcgt gaacaccgca
     3481 caggtgatca tcgatcagtt catttcatcg tcggaagaca agtggcatcg cctgagcggc
     3541 ttggtgatgc tcctgccaca cggtatggag ggtcaaggtc ccgagcattc gagcgcaagg
     3601 ctcgaacgct ttctcttgct ctgcgccgaa gacaacatgc aagtcgtcaa tctgacgaca
     3661 ccggcacagc tcttccactg tcttcgcagg caggtgctgc gaccctggcg caagccgctc
     3721 atcgtgatga gccccaagag cctcctgcga caccggcgtg cgatcagcac gatggatgag
     3781 cttgcggagg gcgctttcca gcgaatcatc cccgatgatt ccgcgatcga tccgaagaag
     3841 gttcggcgct tggtcatgtg tacggggaag atctactacg acctcctcga gcgtcgcgag
     3901 cgggacgaag tcgacgacat cgggattatt cgtgtggagc agctctatcc attgcgcgcg
     3961 agcgagcttc agaagcgcct ggctccatat cgcaaggacg tcgacttggt ctgggtccag
     4021 gaggaaccgt ggaacatggg cgcatggtac ttcatgcgcg cgcgccttcc cgagatcctc
     4081 ggaaacgaac agccacttcg ttgcatcgcg cgcccagaaa gcgccagccc tgcaacaggc
     4141 tcaggggcct cacacaagct cgaacagaag cttctggtcg acgaagtgtt cagctagagc
     4201 ccaacgagcg gccccgtcca tgaacagggc gccgctcgga gcgagactct cagcctactt
     4261 cgatgcattg acggaaatgc tgcgcggggt cggcgcttcc ttcttgggaa gtcgcaatgc
     4321 gagcacgccg tctttcagat ctgcactgat gctgtcggca tcgaccgttt cgggaagggt
     4381 gaaggatcgg gtgaaacttc cgtactgccg ctcgacgcga cgataggtgc cttcggtctc
     4441 ctccttctcg accttacgct cgccgcgaat ggtgagtacg ttcttttcga cgtcgatgtt
     4501 gacgtcatcc gcagtcaagc ctggcacctc cgcatcgacg aaaaacgcat cgtcttcttc
     4561 gcgaatgtcg accgcgactt gaaatgcttg tttcgcaagc cccttgccac tgaagaagtg
     4621 gtcatggaga cgattcatct cttcgaatgg atcccaacga accaacatga ttgactcctc
     4681 cgtcgacccg catgcgcagg cccggtgatg gtcggatcag accgcgacgc caccatcgcg
     4741 tctcggacat gctccggcga ttgaggcaaa ctaagcacgg aaaatgggct gtcaactctc
     4801 gaattgactg ctttttgggg cgcgttaggc ttgcggcgga tgagggattt gcgactggta
     4861 ttcgttctgg cgctcacgac tggcgtagtg tcgctcgggt gcgtcgagga cgttcggttc
     4921 aatccctcgg gctgcgacgt tcacgtgcgc gggcgttggc gggtcgacgg tcaagacccg
     4981 acacccgaag tttgttcgaa catcggtctc gtcgagctcg tcatcatcga cgaaccggag
     5041 ctacagttct ggtcccctcc ggagtttctt ctgaggtgcg aggccgccga tgactccaac
     5101 gccgtggtca ttgacggggg cgcatatctc gacacgctca tcgtcacgcg aaaccgttgc
     5161 gacggaaccg gagcggttct caaggacacg ctcgacgggc aatacaagtc ccgttggcgc
     5221 gcgatcaccg atctgaaatt catcgtcgac tgctcaccca tcgaggtcaa cacgatcacc
     5281 ccgatcgacg gcggaactca attgctcctg gatgtgggga ccgtcgagtt ccaaaccagg
     5341 gacgcgggtc cgatctgccc ttagccgcca tgccttgcgg acgggcccga cgatagctag
     5401 accatcaccg tgtttcgttt tccgccgctg accccgttcg tgaagaagct gctgattgcg
     5461 cttttcgggg cttggattct tcagatcctc cttcagaact gggcgggagt gccgatcttc
     5521 gaggtgctgg ctctcaatac ggctcgcctc ggggttgcaa cgctctggca gctcggcacg
     5581 catgtcctgg ccttccccac gggcccgcag gcgatgtttt cgatgctgtt ttcgctcctg
     5641 ttcctctggt tgatgctcgc tccgttcgaa cagcgctttg ggcagaagcg cacgatccaa
     5701 ctgtgcgtcg tcgcggtgct cgcgtctgcc gccctagcgc tgatggtggg gctactggtg
     5761 ccgagccccc gcgcactgta cggcgcccag gccattcttc tcggatcgat cgcggcgttt
     5821 gcctggtcgt accgaggcca agggcggatg agcttcttcg gcgtgatcga catgaagccc
     5881 gtgcacatca tttacctggt gctcgccctg agtgcgttga tgttcatgct ttcgggcgac
     5941 gccgtggctc tcgcggcgga tgtcggcgcc atcggcggtg gcatgggatt catcgaatgg
     6001 ctgcgccgcc ctccacggcg gagacgaaag aagaagcgga agaagaaggg ctccttggac
     6061 ctggtgcagg gcggccgcaa cgacaaaccc gagtggttga attgacgtgc ttgtccgcgg
     6121 cctgattctg gcgatcttgg gcacgtcgat atccgcgggt gcaacaggct gtgccgacga
     6181 gagcacggtc atcatcccgg tctacgacgc gtgcttcacc gtcgacgagt gcgtcgagac
     6241 cgcaacgctc tgcgaggagc tcgcggtcga cttcgccggc ttcacctact cgaattcgat
     6301 ctgtacgctc acctgtggag cccaaggatc cgtttccccg gactgtccga tagcctgggt
     6361 gggccg
//
LOCUS       DASRZF010000344         2012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1291881, whole genome shotgun sequence.
ACCESSION   DASRZF010000344 DASRZF010000000
VERSION     DASRZF010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291881"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VFG22_15260"
     CDS             complement(<1..587)
                     /locus_tag="VFG22_15260"
                     /inference="COORDINATES: protein motif:HMM:NF013663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HET6417655.1"
                     /translation="MRPRVLVIYKKSTYQRYVGRAQERLKALIAHSDVSVDGLLHEHE
                     IHQETLRRAKKALRDLGARAVLRYRPEPMPEEGSWDMIVTLGGDGTLLWASHLASSST
                     PMLAINSAPDTSVGYFCAGDGHNVDEVLGAALEGKLKSSRLARMRVDVGDETLSTRVL
                     NDALYCHESPAATSRYIIQHGGQQERQMSSGVWVGP"
     gene            complement(624..1271)
                     /gene="fsa"
                     /locus_tag="VFG22_15265"
     CDS             complement(624..1271)
                     /gene="fsa"
                     /locus_tag="VFG22_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HET6417656.1"
                     /translation="MQIFIDSADIEEIRDAAAMGVIDGVTTNPSLVAKTGRPFKDVLV
                     DICEVVDGPISAEVIATDYEGILKEARVLAEMHGNIVVKVPLIAEGLKAVRTLTDEGI
                     RTNVTLCFSPTQGLLAAKAGATYISPFIGRMDDIGGNGMELVEQLVTIYQNYGMHTQV
                     LAASIRHPVHVVQASLIGAHVATIPHKVILQLTKHPLTDLGLERFLADAKKIPTG"
     gene            complement(1355..1510)
                     /locus_tag="VFG22_15270"
     CDS             complement(1355..1510)
                     /locus_tag="VFG22_15270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417657.1"
                     /translation="MRAQSKWTATGVVLWLIAAVAATLLVCSETAPPPETAATPQGDE
                     VEVELVE"
     gene            1579..>2012
                     /locus_tag="VFG22_15275"
     CDS             1579..>2012
                     /locus_tag="VFG22_15275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417658.1"
                     /translation="MRSFLTVLVFLLTWSSAALAENRSGEVDATVARLGSLDPAERAL
                     LVPYLAQGPVVLTEFQNRQTDLPAVIYAARIEAPAETVAGVIGNPKEYPRFMRALTEV
                     DVKSVNGQMMAFDWTWGVTLFSMSGQNVMTTYPGDSDQGYRF"
BASE COUNT          367 a          616 c          632 g          397 t
ORIGIN      
        1 gggcccaccc agactccgct cgacatctgg cgctcttgct ggccgccgtg ctggatgatg
       61 taccgtgacg tcgcagccgg gctctcgtga cagtagaggg catcattgag cacccgtgtc
      121 gacagtgttt cgtcgccgac gtcgaccctc atccgcgcca gacggctcga cttcaatttc
      181 ccttcgagcg cagcgccaag gacttcgtcc acgttgtgcc cgtcacctgc gcagaagtag
      241 ccgacgctcg tatccggggc gctgttgatc gccaacatcg gcgtcgacga gctcgcgaga
      301 tgcgaagccc agagaagcgt cccgtctccg ccaagcgtca cgatcatgtc ccatgaaccc
      361 tcctcgggca tgggctcggg acggtaccgg agcactgcgc gcgcgcccaa gtcgcgaagt
      421 gccttcttgg cccgacgcag cgtttcctgg tgaatctcgt gttcgtgaag gagaccgtcg
      481 accgagacgt cgctatgcgc gatgagtgcc ttcagccgtt cctgtgcacg acctacgtaa
      541 cgttggtacg tgctcttctt gtagatgacg aggactctgg gacgcatgcg tgactcgtgg
      601 tgaggttcgc gatctgccgc gccctacccg gtcggaatct tcttggcgtc cgcgagaaag
      661 cgctcgaggc cgagatccgt gagaggatgc ttggtcaact gcaggatgac cttgtgggga
      721 atggtcgcga cgtgggcgcc gatcaacgag gcctgcacca cgtggacggg gtgccgaatg
      781 cttgccgcga gaacctgcgt gtgcatgccg tagttttggt agatcgtgac gagctgctcg
      841 acgagctcca tcccgtttcc gccgatgtca tccatgcggc cgatgaaggg gctgatgtag
      901 gtcgcacccg ctttggcggc gaggagcccc tgcgtcggac tgaaacagag ggtcacgttg
      961 gtccgaatgc cttcatcggt gagcgtccga acggccttta gaccttctgc gatcagaggg
     1021 actttgacga cgatgtttcc gtgcatctcc gcgaggaccc tggcctcctt cagaatgccc
     1081 tcgtagtcgg ttgcgatcac ctcggcgctg atcggtccgt ctacgacctc gcagatatcg
     1141 accagcacat ccttgaacgg gcgccccgtc ttcgcgacca gcgaagggtt ggtcgtcacg
     1201 ccatcgatga cgcccatcgc ggcggcatcg cggatctctt cgatgtctgc ggagtcgatg
     1261 aaaatctgca tggggctagt aaagagcgat ggcctcgcag ggtcaagcag agcctcatcg
     1321 aagccaaatc gcgaccgaga aaccccggat ccactcactc cacgagctcg acctcgacct
     1381 catcgccctg aggggtggcg gcagtctcgg gtggaggcgc cgtttccgag caaaccaaca
     1441 gggtcgcggc gacggcggcg atcagccaca atacgactcc ggttgcggtc catttgctct
     1501 gcgcgcgcac ggccgggctc tccgaggacc gttttgcaac gggattgaag aacgagcaac
     1561 cgaggccgtc caagcgacat gcggagtttt ctcacagttt tagtcttctt gctgacctgg
     1621 agcagtgccg cgctggccga gaaccgaagc ggcgaggtcg acgcgaccgt cgcgcgcctt
     1681 ggcagcctcg accccgcgga acgggcgctg ctcgtaccct atctggcgca aggacccgtc
     1741 gtgctgacgg agtttcagaa tcgccaaacg gatctgcctg cggtcatcta tgcggcgcgc
     1801 atcgaagctc cagcggagac cgtggcgggg gtcatcggga atccaaagga gtatccgcgc
     1861 ttcatgcgtg cattgaccga ggtcgacgtg aagtcggtca acggtcaaat gatggccttt
     1921 gactggacct ggggagtcac gctcttttcg atgtcgggtc aaaacgtcat gacgacgtat
     1981 ccaggcgata gcgatcaggg ttaccgcttc ga
//
LOCUS       DASRZF010000345        13123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4732089, whole genome shotgun sequence.
ACCESSION   DASRZF010000345 DASRZF010000000
VERSION     DASRZF010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 13123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13123
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4732089"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..1228
                     /locus_tag="VFG22_15280"
     CDS             248..1228
                     /locus_tag="VFG22_15280"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HET6417659.1"
                     /translation="MGASVGIDVSKRCLDWALGSEARSTRTLNTQSGIRKLSEQLSKH
                     SVDRIIVESTGGDERPVVDALASVGLPVIVVNPWRVRRFAEGLGIHAKTDALDAQILA
                     LFGERVNPTQRPIPGPRERELADLVQRRRQLIAMVVAEKNRLEHGSEAVRRDIVSLIQ
                     VLERRIAKLDRAIDQAIAKDEVSAEKRVQLESVPCVGPGIARALIVDLPELGTLGRRQ
                     IASLVGVAPFANDSGKKSGYRRIRAGRSGPRTALYLAAMNASRFNSALKPVYDRLVAA
                     GKPRKVALIAVARKRLTILNAMVRDHALWRETTAQSELSATVACWAAPSI"
     gene            1262..1492
                     /locus_tag="VFG22_15285"
     CDS             1262..1492
                     /locus_tag="VFG22_15285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417660.1"
                     /translation="MGEPKRATVYFDMKIHRALRLKAAETDRSVSDLVNEAVRESLSE
                     DAADLGAFKERSRERRHKFEDVLKQLKRRGAI"
     gene            1479..1742
                     /locus_tag="VFG22_15290"
     CDS             1479..1742
                     /locus_tag="VFG22_15290"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HET6417661.1"
                     /translation="MARYSVEIRTSAIKELETVTPKRERQRIARKISTLADEPRPRGC
                     EKLAAQGDRYRVRQGRYRIIYAVDDARLLVDVVKVGHRKEVYR"
     gene            complement(1873..2304)
                     /locus_tag="VFG22_15295"
     CDS             complement(1873..2304)
                     /locus_tag="VFG22_15295"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6417662.1"
                     /translation="MLRGGINHLDLTVRDPRISEPFYRAVLGFLGFEPKRLAHGNTHL
                     PLFHSTEGERLFSIALQQAKHEAEHDRYSPGLHHVAFSAASRDDVDSLHHLLMEMGAI
                     VLDPPAEYPQYALGYYAVFFADPDGLKLEFVHMPSEPEFAA"
     gene            complement(2626..3792)
                     /locus_tag="VFG22_15300"
     CDS             complement(2626..3792)
                     /locus_tag="VFG22_15300"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HET6417663.1"
                     /translation="MSKQTAAASTIQISQEQQTLMAAMELGETGWLLGFASAYGQKPL
                     RRKIGSRDRRALMREIERAKAVLGLEPGVRVVSCYEAGRDGFWLHRFLKSEGVESLVV
                     DSSSIEVNRKKRRAKTDRVDLVALLDLLSRHLAGSAKPVWSVVRVPSVEDEDRRHLHR
                     ELKLAKKDRTRVSHRMKGLLSNHGLTLDLRVDLAAQLAVMRQWDGSPLPPGLRGRLER
                     YLGEYQYFTNRIHDLDSERRRILREEKSPLLEKVLQLYSLRALGINSSWMLTTEFFGW
                     RDFRNGKQVGSMAGLTPTPFNSGGGEREQGIGKDGSRWVRGGAIELAWGWLRFQPQSA
                     LSQWYMRRFGSGSKRLRKIGIVALARKLLIALWRFLETGVVPDGALLKMDLRLR"
     gene            complement(3997..4518)
                     /locus_tag="VFG22_15305"
     CDS             complement(3997..4518)
                     /locus_tag="VFG22_15305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417664.1"
                     /translation="MDLTEALLGIAALAVALAGFAGVAIALSGEAHRNVGDRYRIICL
                     LSSTLPIIPLALLPLVLLFWPVSSAITWRVASALGVLHCTGFAYLMRRNLREYRDHPD
                     FPIMYRTTWTIIAVPIILVVQLVQFMNAVGWPIDPNPGAFLLNLMVWVSYAAAVFADI
                     IFVRPSGGPGATA"
     gene            4727..5329
                     /locus_tag="VFG22_15310"
     CDS             4727..5329
                     /locus_tag="VFG22_15310"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET6417665.1"
                     /translation="MNNNTGGDPLKKITLVGIDVSARKLDLALEPNRGKPLALAFDNT
                     PVGHRKLVKRIKRAGHATRVCLEATGVYHLDLCLALHSAQGIEVMVVNPRAAKDFARA
                     QLQRSKTDQVDARSLLEFLRRMDFAPWNPPSRSIRDLRALSRRIQSLVVSRAQEKNRR
                     HADEVVSDCVHRSIVITQIGHRDHSGAEAAPIGRSVIVNT"
     gene            5474..7024
                     /gene="istA"
                     /locus_tag="VFG22_15315"
     CDS             5474..7024
                     /gene="istA"
                     /locus_tag="VFG22_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HET6417666.1"
                     /translation="MASPRLSMDKIREILRQKWSLGRSHREVARSQGVSVGMVGATLR
                     RARRAGLEDFASIEPLTETALEAQLYGPARSGSRPVPDPLWIHTERQRKGVTLELLHH
                     EYLEQHPEGYRYTQFCEHYRRWCKKRRLSMRQVHRAGEKLFVDYAGHKPKVIDRTTGE
                     EREVELFVATFGASSFTYAEATETQRVADFIGSHSRALTFFGGVPELVIPDQLKSGVT
                     RACRYEPGLQRSYDEWSRHVGTTIVPARPRKPRDKAKVEVAVQVAERWILARIRNESF
                     FSLGELNHRIAELLEGLNGRSMRAYGQSRRERFEAIDRPALRPLPTEAFELAEWKHAR
                     VNLDYHVEFNRCFFSVPHALVHERVEIRSTATALEVFCKGRRVALHHRPTYYAPGGYS
                     TIPEHMPKAHRKHLEWTPTRLIHWGASVGPKTAELVESILEHRPHPEQGYRSCLGILR
                     LAKQYGNERLEAACDRAVLVRARSYRHVQSILKNGLDRVPPATKSAPTQRALPLTHEN
                     VRGGDYYN"
     gene            7048..7788
                     /gene="istB"
                     /locus_tag="VFG22_15320"
     CDS             7048..7788
                     /gene="istB"
                     /locus_tag="VFG22_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HET6417667.1"
                     /translation="MLMQPTLEQLNELRLHALARAWQAQHEDPSLDDLGFDDRLALLV
                     EAEWTDRQNKRLSRLLREAKLRIAGACLEDLEYAKERKLERPLMRQLATGRWIEAHQN
                     VVITGATGVGKTYVACALAQQACRRGHRVLYRRAPRLFDELTLTRADGTYAKLLARFA
                     RTDVLVLDDWGLAPVHDVERRDLLEIMEDRHGLRSTIWTSQLPVTQWHDHLGDPTVAD
                     AICDRLLHNAHRIVLQGPSRRKTEANKD"
     gene            7948..8430
                     /locus_tag="VFG22_15325"
     CDS             7948..8430
                     /locus_tag="VFG22_15325"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET6417668.1"
                     /translation="MAAINEEIETLTETALEVVRTDPSLSSKLEHLISVKGIAVASAI
                     AILAELALLPSDMSARQWVAHAGIDPRYAESGSSVRGQVRISKVGNRHLRAALFLPAM
                     VASQHEPRIKAYYQKLIAKGKKPMQALVAIMRKLLHAIHGMFRHDADFDGSRFLAERA
                     "
     gene            complement(8650..9045)
                     /locus_tag="VFG22_15330"
     CDS             complement(8650..9045)
                     /locus_tag="VFG22_15330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417669.1"
                     /translation="MNWDAIGAIGEAIGAAGVIASLLYLAVQVRASTRASAIESKLAS
                     SRIYTGFLGSLIQSPELNRLFVQGRHDLASLSSEDDYYRFSNLALQSFSHFSATYFQY
                     RQGTLSASDWFESRAVIHYWLRGAGCQAW"
     gene            9280..10275
                     /locus_tag="VFG22_15335"
     CDS             9280..10275
                     /locus_tag="VFG22_15335"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HET6417670.1"
                     /translation="MKKPLLCGIDVGSNELVVAIDPGTGKVWDGTFPNDGTGHRKLIR
                     RLSRTKAAARICVEATGIYHLDLCLALDQAKGIEVMVANPRTTKDFARAQLRRSKTDR
                     TDALSLLEFVRRMNFEPWSPPGRPILSLRALSRRIQALLVSRAQEKNRLHADESVEQT
                     PAAIRDSIDRHIDAINIEIETLTEAALDLVRADDTLGSRFAHLVSVKGIAQASAITLL
                     AELSVLPADMSTRQWVAHAGIDPRHYESGTSVSAAVRISKVGNRHLRAARFLLAMVAS
                     QHEPQVRAYYEKLLAKGNKPMQALVAVMRKLLHAIHDMFKHDADFGGTKFFAKKT"
     gene            complement(10435..10833)
                     /locus_tag="VFG22_15340"
     CDS             complement(10435..10833)
                     /locus_tag="VFG22_15340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HET6417671.1"
                     /translation="MGLPRSVVRIANAITNAAIITGVPRPPYTRRNALVIETIGRRSG
                     KRRRIPVGYLDDNGRIIVVVEDGASSHWVQNALAQDGLRIHLRGEWKSAKLRMLDVEP
                     EAYLCRMNRFHAGLVRSHSSTPSVAELVLE"
     gene            complement(11020..11352)
                     /locus_tag="VFG22_15345"
     CDS             complement(11020..11352)
                     /locus_tag="VFG22_15345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417672.1"
                     /translation="MSHGVPESDWKVFRELREVALERLCERILDEVATVISNSSRTHH
                     ERFGELFGLIRDRNHDIARGFDGPKRSAMLAQLSVNYSLDLLKPSDMARFSAETRESV
                     ESLANLRR"
     gene            11594..12537
                     /locus_tag="VFG22_15350"
                     /pseudo
     CDS             11594..12537
                     /locus_tag="VFG22_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(12602..12985)
                     /locus_tag="VFG22_15355"
     CDS             complement(12602..12985)
                     /locus_tag="VFG22_15355"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET6417673.1"
                     /translation="MNADDARSFAEKWLPAWTGNRPELLASFYTPDTVYSDPAIPAGV
                     RGRDALLAYFSKLLAKNPEWVWVHRGSIPIEDGFLNLWHASVPAGQHTVEAEGVCTVQ
                     LRAGLIYANHVFFDRSELLTALSSS"
BASE COUNT         2644 a         4204 c         4027 g         2248 t
ORIGIN      
        1 gagctgttgg gtgtcagctg caaccgctct tatggcggac ccgtggtaga agtgggtagt
       61 cggcaggtag ctgcaacgac ctgccgacct ttacccggag tcggcagacc gcaaacgcct
      121 cggtcccgta tgagaccgtc aacaagcaag gggcaccgtg ctccattgct gcagcgaagc
      181 tcgaacggtt gcctgggcca tgagcccgta tccaagagtc cgagttcgtt gttctggaga
      241 gcataccatg ggcgcttctg ttggaatcga tgtctccaag cggtgtctcg actgggcgct
      301 aggcagtgag gcaagatcga cccgaacact gaatactcaa tcgggcattc gtaagctcag
      361 cgagcaactg agcaagcact ctgtcgatcg gatcatcgtc gagagtacgg gcggcgacga
      421 gcgtcccgtg gtcgacgccc tcgcgtcggt cgggctaccc gtcatcgtgg tgaacccgtg
      481 gcgggtgcgc cgcttcgccg aggggctggg tatccacgcc aagaccgacg cgctcgatgc
      541 gcagatcctc gccctcttcg gcgagcgtgt gaatccgaca cagaggccca ttccaggccc
      601 gagagagcgc gagctggccg atctcgtgca gcgacggcgt cagctcatcg ccatggtcgt
      661 agccgagaag aaccgcctcg agcacggctc cgaagcggtg cggcgcgata tcgtctccct
      721 catccaggtg ctcgaacggc gcatcgcgaa gctcgatcgc gcaatagacc aggctatcgc
      781 caaagacgag gtgagcgccg agaaacgcgt gcagctcgag agcgtgcctt gtgtcggccc
      841 gggcatcgcg cgggcgctca tcgtcgacct cccagagctc gggaccctcg ggcggagaca
      901 gatcgcttcg ctggtcggcg tcgcgccctt tgcgaacgac agcggcaaga agagcggcta
      961 ccgccgaatt cgtgccggtc ggtccggtcc ccgcaccgcg ctctatctcg ccgcgatgaa
     1021 cgcttcgcgc ttcaactcgg ccctcaaacc agtctacgac cgactggtcg ctgccggtaa
     1081 accacgcaag gttgccctga tcgcggtggc tcggaagcgc ctcaccatcc tcaatgcgat
     1141 ggtcagagac cacgcgttgt ggcgcgaaac gaccgcccaa tccgaacttt cagccacggt
     1201 tgcttgttgg gcggctccct cgatctagcg ttataacgtc aaagcgctat aatggattgc
     1261 catgggagaa ccgaagcgag cgaccgtgta cttcgacatg aagattcacc gagcacttcg
     1321 gctgaaggca gcggaaacgg accgctccgt atcggatctg gtgaatgagg ctgtgcggga
     1381 gagcctttcg gaggacgccg ccgatctggg cgcattcaag gagcgatcga gggagcgccg
     1441 ccacaagttt gaggacgtgc tgaaacagtt gaagcgtcgt ggcgcgatat agcgtcgaga
     1501 tccggacgtc cgccatcaag gaacttgaga ccgttacacc gaagagggaa cggcagcgga
     1561 tcgcgcgaaa gatctcgacg ctcgcggacg agccacgccc tcgcggttgc gagaagctcg
     1621 ctgcccaagg ggaccgatac cgcgtccgcc aggggcgcta ccggatcata tacgccgtag
     1681 acgacgcgag attgctcgtg gatgtcgtga aggtcggcca ccgcaaggaa gtctatcgct
     1741 gagccgccca acggactggg cgctcagctg caccggaccg cgctgaccgg ctcgcttagc
     1801 cccggattga gacgccagag agtaccacgc ctcgatcacg gcgtgcggtt cgctgtcagc
     1861 tgcagcgact tgttaggcgg cgaactcggg ctcactcggc atgtgaacga actctagctt
     1921 gaggccgtcc ggatcggcga agaacactgc atagtagcca agcgcgtatt gcgggtactc
     1981 cgccggagga tcgaggacga tcgcacccat ctccatgaga agatggtgga gactgtccac
     2041 gtcgtcacgg ctcgccgccg agaaggcgac atggtgtagg cccgggctgt agcggtcgtg
     2101 ctcagcttcg tgtttggctt gctgcagcgc gatcgagaac aggcgctcgc cttctgtgga
     2161 gtggaagagc ggaagatgcg tgttgccgtg ggccaaacgc ttcggctcga agcccaagaa
     2221 ccccaggaca gcacggtaga agggctccga tatcctggga tctctgacgg tcagatcgag
     2281 atgattgatt ccacctcgga gcatgcttcg ccgccttcta tgtaggccgt gctgtcaacc
     2341 tccatggtcg acgatcctga ttctacatgg atgttgctcc caagtgcatg ctcgaccgtc
     2401 gcgctctcga cctggatcct tggcggcttc ctgaagcgtc gcggctatgc cgcatacgac
     2461 cttctatccg tgtcgatggc gttgcatcga gcacccgagc ctttgtacgc acactgcgtg
     2521 ggtgaagcct cttggcagcc agccccatct aacgggcggg gtctctagga cacctggcgg
     2581 ctattcgagc cgactgcacc aagatccggt tccctttttg gatccctaac ggagtcggag
     2641 gtccatcttc aacagagcgc catccggcac cactcccgtc tcgaggaagc gccacagcgc
     2701 gatcaggagc ttccgcgcca gcgctacgat cccgatcttg cgaaggcgtt tgctccctga
     2761 accgaagcga cgcatgtacc actgcgacaa cgcactctgc ggctggaaac gaagccaacc
     2821 ccacgccaac tcgatcgctc ccccgcgcac ccagcgactg ccgtcctttc cgattccttg
     2881 ctcccgctca cccccaccac tgttgaaggg cgttggcgtc agacccgcca tcgaacccac
     2941 ctgcttcccg ttacgaaagt cccgccagcc aaagaactcc gtcgtcagca tccaggagct
     3001 gttgatcccc aaggcacgaa gcgagtacaa ctgaagaacc ttctccagca gcgggctctt
     3061 ctcttctcga aggatccggc gacgctccga gtccaggtcg tggatccggt tcgtgaagta
     3121 ctggtactcc ccgagatagc gctccagacg cccgcgcaga cccggcggca gcgggcttcc
     3181 gtcccattgt cgcatcacag cgagctgcgc agcgagatcg acgcgaaggt ccagcgtcag
     3241 cccatggttc gagagcaagc ccttcatgcg gtgactcacc cgggtgcgat ccttcttcgc
     3301 cagcttgagc tcgcgatgaa gatggcgccg gtcttcgtcc tccaccgagg gcacccgaac
     3361 cacgctccac accggcttcg cacttcccgc caggtgacgc gacaagagat cgagcagcgc
     3421 caccaggtcg acgcgatccg tcttcgcccg gcgcttcttg cggttcacct cgatgctgga
     3481 agagtcgacc accagactct ccactccttc ggacttcaga aagcggtgaa gccagaagcc
     3541 gtctcgaccg gcttcgtagc agctcaccac acgcactccc ggctccaggc cgagcactgc
     3601 cttcgcccgc tcgatttccc gcatcaatgc tcgccgatcc cgagagccga tcttccgccg
     3661 cagcggcttc tggccgtacg cgcttgcgaa accgagcagc cagcccgtct cgcctagctc
     3721 catcgccgcc atcagggtct gttgttcttg gctaatctga atcgttgagg ccgctgctgt
     3781 ttgcttggac atcgtgtccc tccattgtgt ggttacaagg aggtttacag cagtggcctt
     3841 ctctatccgt gagcccatgg ggcataggct ctaacggcta tggcgttcag ctgcaggcgg
     3901 ggtactagcg cccaagcggc gagaacgccc agaagcacgc agggtaccac agcacccgat
     3961 gggccgacgc tccagcctgt ctgctacaac gccttgttag gcggtagccc ccggtcctcc
     4021 gctgggccga acgaagatga tgtcagcgaa tactgcagcg gcgtaagaca cccagaccat
     4081 cagattgagc aggaatgcgc ccgggttggg atcgatgggc cagccgacgg cgttcatgaa
     4141 ttgcacaagt tgcactacga gaatgatcgg aacggcgatg atcgtccatg tcgtacgata
     4201 catgatcggg aaatcagggt ggtcccggta ttctctcaaa tttcgccgca tcagataggc
     4261 aaagccggtg cagtggagca ctccgagggc actagcgact ctccaggtaa tggccgagga
     4321 cacgggccag aagagcaaca ccagaggcag cagagccaat gggataattg ggagggtcga
     4381 actgaggaga cagattatcc tgtatcggtc accgacgttg cgatgcgctt cgccagacaa
     4441 cgcgatagcc acgccagcga agcctgcgag cgcaactgcg agcgccgcga tgccaaggag
     4501 cgcctcagtg aggtccatcg ctctgccgcc ttgtatatcg ctcccacggc ctctcgcgag
     4561 atgcgacgct agtgattcat ccggggagat cgttgccgcc tgaggccccc gagcctgtgc
     4621 gttagatgga tcccccgccc tctttgccca tgccgaggag tatctgaggc ccgagagcct
     4681 gcatctacaa gaccggtagg caacggttca gggtcagggg acccggatga acaataacac
     4741 cggaggggat cccttgaaga agatcacgct cgttggcata gatgtcagcg cccgcaagct
     4801 cgacctggct ctcgagccga atcgcgggaa gccattggcc ctggctttcg acaacacgcc
     4861 agtcgggcac cgaaaacttg tcaagcggat caagcgtgcc ggtcacgcga cccgcgtctg
     4921 cctcgaggcg actggtgtct atcacttgga tctctgtctc gccctccact cggcccaagg
     4981 catcgaagtc atggtcgtca atccccgggc cgcaaaggac tttgcacgtg cgcagctgca
     5041 acgctcgaag acagaccagg tggacgcgcg ctcgctcctc gagttccttc gccggatgga
     5101 cttcgcgccc tggaatcccc ccagccgctc gattcgggac ctacgggcac tctctcggcg
     5161 aattcagagc ctcgtggtga gccgcgcaca ggagaagaac cgacgccatg cggacgaggt
     5221 cgtctccgac tgcgtacatc ggtccatcgt gatcacccag atcggtcatc gtgatcactc
     5281 cggggccgag gcggctccga tcggtcgatc ggtcatcgtg aacacctaga tcggagtcgt
     5341 gatcacctag atcgggatcg tgatcacgcc gatcggtgat cgtgaacggt gcattctccc
     5401 cccaggtgag agggaggaag gcgtcgctgg acaggccggc ttggcatcga gcctccacga
     5461 aacgggaggc gagatggcaa gcccgaggct gtccatggac aagatccgag agattttgag
     5521 gcagaagtgg agcctggggc gaagccaccg ggaggtggct cgcagtcagg gcgtgagcgt
     5581 ggggatggtg ggcgccacgc tgcgccgcgc ccgcagggcg ggcctcgagg actttgcgtc
     5641 gatcgagccg ctgacggaga cggctctcga ggcgcagctc tacggtcccg ctcggtcggg
     5701 aagccgtccg gtgccggatc cgctgtggat ccacaccgag cgccagcgca aaggcgtcac
     5761 cctcgagctg cttcaccacg agtacctgga gcagcacccg gagggctacc ggtacaccca
     5821 gttctgcgag cactaccgcc gctggtgcaa gaaacgacgg ctctcgatgc gccaggtcca
     5881 ccgtgctggc gagaaactct tcgtcgacta cgcgggtcac aagccgaagg tcatcgaccg
     5941 aaccaccggc gaggaacgcg aagtcgagct gttcgttgcc accttcggtg cttcgagttt
     6001 cacgtacgcc gaagcgaccg agacccagcg tgtggccgac ttcatcggca gccacagtcg
     6061 cgctctgacc ttcttcggcg gagtgcccga gctcgtgatc ccggatcagt tgaagagcgg
     6121 cgtgacgcgc gcgtgtcgct acgagcccgg tctccagcgg agctacgacg agtggagccg
     6181 ccacgtgggg acgacgatcg tgcccgcgcg acctcgcaag ccccgcgaca aggccaaggt
     6241 cgaagtcgcc gtccaggtgg cggaacgctg gatcctggcc cggatccgca acgaaagctt
     6301 cttctcgctc ggtgagctga atcatcggat cgcggagctc ctcgaaggac tcaatggccg
     6361 gagcatgcgc gcctacgggc agagccggcg cgagcgcttc gaggcgatcg accggcccgc
     6421 actgcgcccg ctgcctacgg aggccttcga gctggcggag tggaagcacg cgcgcgtgaa
     6481 cctcgactac cacgtcgagt tcaatcgttg cttcttctcc gtcccccatg cgctcgtcca
     6541 cgagcgcgtc gagatccgct ccaccgcgac agccctggag gtcttctgta aggggcggcg
     6601 cgtggccctc caccaccgac ccacgtacta cgcgccgggc ggctattcga cgatccccga
     6661 gcacatgccg aaggcgcacc gcaagcacct ggagtggacg cccacgcggc tcatccactg
     6721 gggagcgtcg gtgggcccga agacggcgga gctggtcgag tcgatcctcg agcatcgtcc
     6781 gcacccggag cagggctacc gctcgtgcct cgggatcctg cgtctggcaa agcagtacgg
     6841 caacgagcga ctcgaggcgg cctgcgatcg cgcggtcctc gtccgcgcgc gctcctaccg
     6901 ccacgtgcag tcgatcctga agaacggcct cgaccgggtc cccccggcga cgaagtccgc
     6961 gccgacccag cgcgcactgc cgctcacgca cgagaacgtg cggggcggcg actactacaa
     7021 ctgaacgaag acaaggagat cgcccccatg ctcatgcaac cgacgctcga gcagctcaac
     7081 gagctgcgcc tccacgcact ggcgcgtgcc tggcaagcgc aacacgaaga ccccagcctc
     7141 gacgacctcg gcttcgacga tcgactggcg ctgctcgtcg aggccgagtg gacggatcgc
     7201 cagaacaagc gtctctcgcg cctgctccgc gaagcgaagc tccggatcgc aggggcttgc
     7261 ctcgaagacc tcgagtacgc gaaggagcgc aagctcgagc ggcccctgat gcggcaactc
     7321 gccacggggc gctggatcga ggcccaccag aacgtggtca tcacaggggc cacgggtgtc
     7381 ggcaagacct acgtcgcttg cgccctggcc cagcaggcct gccggcgcgg ccaccgcgtc
     7441 ctctatcgcc gagccccgag gctcttcgac gagctcaccc tcacgcgtgc ggatggcacc
     7501 tacgcgaagc tgctcgcccg cttcgcccgt accgacgtcc tggtgctcga cgactgggga
     7561 ctcgcgcccg tccatgacgt ggaacggcgc gacctgctcg agatcatgga agaccgccac
     7621 ggcctccgct cgacgatctg gaccagccag ctcccggtga cccagtggca cgaccacctc
     7681 ggcgatccga ccgtcgccga tgccatctgc gatcgactcc tccacaacgc ccaccgcatc
     7741 gtgctacaag gaccctcgcg aaggaagacg gaggcgaaca aagactgaaa acccgtccag
     7801 cgataccaga caccgatcac gatcaccgat ctgggtgtcc acgatcaccg atctacgtga
     7861 tcacgatgaa ccgatctggg tgatcacgat cgccggaata cgcactccga cacaacgcct
     7921 gcgatccgag agagcatcga gcggcatatg gccgcgatca acgaagagat cgagacactc
     7981 acggaaactg ccctggaggt cgtccggacg gacccaagtc tctcttccaa gctcgagcat
     8041 ctgatctccg tcaaggggat cgccgtggcc agtgcgatcg caatccttgc cgagctcgcg
     8101 ttgcttccgt ccgacatgtc ggcacgacag tgggtcgctc acgcagggat cgatccccgc
     8161 tacgcagaat ccgggtcgtc ggtccggggg caggtccgga tcagcaaggt ggggaaccga
     8221 catctgcggg cggcgctgtt tctgcccgcg atggtggcgt cgcagcacga gccccgaatc
     8281 aaggcgtact accagaaact gatcgcgaag ggaaagaagc cgatgcaagc gctggtggcc
     8341 atcatgcgaa aactgctcca cgcgatccac ggcatgtttc gacacgacgc agacttcgat
     8401 ggaagtcggt tcctcgcgga aagagcctga ctggagacgg agtatctaac ggatcggcgt
     8461 tcagcggctt gcggcggcct gcacgcagct gcgaaacacc tgggatcaac gcgatgccat
     8521 cacgaaccaa ttcgactcaa gcgttagcaa gtccgctgca acgtcttgtt aggcagcatc
     8581 gataccgtgg atctccgagt cgatgaaggt gacgaaatca ggtccgaaca tgtgcttgcc
     8641 gagcttgtgc taccacgcct ggcatccggc gccgcgaagc cagtagtgaa ttacagccct
     8701 gctctcgaac cagtcagagg cgctcagcgt tccctggcga tactggaagt acgtggcgga
     8761 gaagtgcgag aaagattgaa gcgcaaggtt cgagaatcgg tagtaatcgt cttcggagga
     8821 cagagaagcc agatcgtggc gtccctgcac aaagaggcgg ttgagctcgg gcgactggat
     8881 cagcgaaccc aagaagccgg tgtagattcg gctcgaggca agttttgatt cgatcgcgga
     8941 ggcccgcgtg ctggcgcgaa cctgcaccgc gaggtacagc agcgatgcaa ttacgcccgc
     9001 tgctccgata gcctctccga ttgctccgat tgcgtcccag ttcacgccta tgctgccttg
     9061 tatatctccc ccgcctagcg aacgagatga gatagttcag tttcgtccgg ggagatcgtt
     9121 gctgcctgag gccccgagcc tgtccgttag atggatcccc cgccttcttt gcccatgccg
     9181 cggagtatct gaggcccaga gagcctgcat ctacaaggtc ggtaggcaac ggtacaaggt
     9241 cgggggaccc ggaggtcagc ctaccaccga ggagccctca tgaagaagcc tcttctctgc
     9301 gggatcgatg tcggctctaa tgagctcgtc gtcgccattg accccggcac cggaaaggtt
     9361 tgggacggga ctttccccaa cgatggcacc ggccaccgca agctgatccg tcgtctgtcc
     9421 cgcaccaaag ccgcggctcg catctgtgtc gaagcgaccg gcatctatca cctcgatctt
     9481 tgcctagcac tcgaccaggc gaagggcatc gaggtcatgg tcgcgaatcc tcgcaccacg
     9541 aaggacttcg cacgcgcgca gctgcgacgc tcgaagaccg accgcacgga tgcgctctcg
     9601 ctgctcgagt tcgtgcggcg catgaacttc gagccctggt cgcccccggg ccgtccgatt
     9661 ctgagcctgc gcgccctctc gaggcgaatt caagcccttc tcgtgtcgag ggcccaggag
     9721 aagaatcgcc tgcacgcaga tgaatccgtg gagcagacgc cggcggccat ccgcgacagc
     9781 atcgatcgac acatcgatgc gatcaacatc gagatcgaga ccctgaccga ggccgctctc
     9841 gatctcgtgc gcgccgacga cacgctcggc tcgcgcttcg cccacctcgt gtccgtgaag
     9901 ggcattgccc aggccagtgc gatcacgctg ctggccgagc tctcggtgct cccggccgac
     9961 atgtcgactc gccagtgggt ggctcacgcc gggatcgacc cgaggcacta cgaatcaggc
    10021 acttcggtga gcgcggctgt ccgcatcagc aaagtgggaa atcgccacct tcgcgcggcg
    10081 cgcttcctgc tggccatggt ggcgtcgcaa cacgagcccc aggtccgcgc ctactacgaa
    10141 aagctgctgg cgaaaggaaa caagccgatg caggcgctcg tcgcggtcat gcgaaagctg
    10201 ctccacgcga tccacgacat gttcaagcac gatgccgact tcggtggaac gaagttcttc
    10261 gcaaagaaga cttgaccggg aacggagtat ccaacggatc ggcattcgcc ggccggcaac
    10321 agcgctgacc gggtagccga agtcgaccca gcatgacgcc agacggcaac caaggtcaat
    10381 tgaaccgagc tggtgccggg ccggtgcaat aacttgttag ccggccgttc atcgctactc
    10441 gaggacgagt tctgcaacgc ttggagtgga tgaatggctt cggaccaggc ccgcgtggaa
    10501 gcgattcatg cggcacaaat acgcttcagg ctccacgtcg agcatccgga gcttcgccga
    10561 tttccattcg cctcgcaggt gaattcgcag accgtcttga gcgagggcgt tttgaaccca
    10621 gtgggaactc gccccgtctt ccacgacgac aatgatcctt ccgttgtcgt cgagataccc
    10681 aacggggata cggcggcgct ttcccgagcg cctgccgatt gtctcgatga ccagcgcgtt
    10741 ccttcgcgtg tagggcggcc gaggtacgcc cgtgatgatc gccgcgttcg tgatggcatt
    10801 tgctattcgg actacggatc gcggaagtcc cataaggccc cgctgtgccg gctaacggat
    10861 cggcgtcaac cggccggcaa cagcgctgac cggctacgcc aagacaccga agtatgccgc
    10921 cagacagcaa ccatggtcaa ttgaaactgg ctgttgaccg atccgcgttc aacgccttgt
    10981 tatacggctg ttcgcgatga gcacgttttg gccgcggaac tatcgcctga ggttcgcgag
    11041 ggactcgacg gattcgcggg tttccgcgga aaagcgcgcc atgtcgctcg gtttgagtag
    11101 gtcgaggcta taattgactg agagctgcgc gagcatcgcg gatcgctttg gtccgtcaaa
    11161 accacgtgct atgtcgtgat tgcggtcgcg aatgaggccg aagagctcgc cgaatcgctc
    11221 gtggtgcgtg cggctggaat tcgaaatgac agtcgcgact tcgtcgagaa ttcgctcaca
    11281 cagacgctca agcgcaacct cgcgaagctc gcggaacacc ttccagtctg actccgggac
    11341 tccatgtgac atcggcttct tgccgtataa cggacacgcc gttcagcggc cggcccgcgg
    11401 gaacctcccg ccggagctgc tgaatggccc tgccagaggg taccacggtc gggacggcga
    11461 acggtggccg gtccgctgca acggcttgtt atgcggggcg gattcataaa ctaagtgcaa
    11521 cagggcactc cgaagaagag gtgacctggg atacgccact ctttctcaaa cgaccaagcc
    11581 tgaggaagag ctcatgacct atcaccagat caccctcgag gaacggtacc ggatcgcggc
    11641 gctgcgtcag gaggatcagg ctccggccga gatcgcgcgg cgtgtcggaa ggcaccggag
    11701 caccatttcc cgggaactga tgaggaaccg ctctcgctgg gacgagagct atcggcccag
    11761 gagcgggcca acgggtgccg ctcccggtcg cgccgcaacc ggcgcttcgg atctcgagac
    11821 tgtaggctcg tcgagtcctt gctgcgcagg cagtggagcc ctgaacaggt gtcgggccta
    11881 ctccagaaga gccggaagct ctcgatcagc cacgagacca tctaccgcta cgtctgggac
    11941 gaccgagtcc ggggaggctc ggtgtaccag gacttccgcc aggccgcgaa gaagcgcaga
    12001 aagcgatatg gcacctacga cagccgaggg cgactcgccg gcaagcgaca catctcggag
    12061 cgacctcagg tcatcgaaca gcgcacacag cccggtcact gggagatcga cacggtcatg
    12121 ggcaacggcg ccacgcactg cgtgctgact ctcgtcgaga gagcgacagg cttcgtcgtg
    12181 atcggaaagc cgaagacgag aggaagccac gagacggcgc gcaaggcgat ccagctcatc
    12241 cgccgctacc ccggaaactt caagaccatc acctcggaca acggaacgga gttccacgcc
    12301 tataagcgcg tagagaaggc gacgggcgtc aagttctact tcgccacgcc ccaccacgcg
    12361 tgggagcggg gaacgaacga aaatgcgaac ggtctgatcc gacagtatct acccaagcga
    12421 accagcatgg cccacgtgac acagcgcgat tgcaacacca tcgcgaagaa gctgaacact
    12481 cggccgcgaa agagatacga ctacgacacc ccggaggagc gcttccatgc cgtctagaga
    12541 atgttgcagt tcaaacttga tactaagcgg ccacccaagc gttcggctcg cttccccccg
    12601 actacgaact ggagagcgct gtgagtagct ctgatcggtc gaagaagacg tggtttgcgt
    12661 agatcagacc agcgcggagc tgaaccgtgc acacgccctc tgcctccacg gtgtgttggc
    12721 cggctgggac agatgcgtgc cacaagttca ggaagccatc ctcgatcggg atggagccgc
    12781 ggtgtaccca cacccactcg gggttctttg cgagaagctt gctgaagtac gctaggaggg
    12841 cgtcgcgacc acggacacct gccggaatcg ccgggtccga gtagaccgta tccggcgtgt
    12901 agaacgaggc gagcagctct ggccgattcc cggtccaggc aggcagccac ttctccgcga
    12961 acgagcgtgc atcgtccgcg ttcattccgt gaaactgcat agacccccgc gtggccgtct
    13021 aacggatcgg gcttcagctg caacagacag cgctgaccgt cagcgctatg ccacagaagt
    13081 cccgacgcca gaatgctaca gaattcagga acgcccagct gtc
//
LOCUS       DASRZF010000346        22934 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4732936, whole genome shotgun sequence.
ACCESSION   DASRZF010000346 DASRZF010000000
VERSION     DASRZF010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 22934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22934
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4732936"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /gene="aroC"
                     /locus_tag="VFG22_15360"
     CDS             complement(<1..674)
                     /gene="aroC"
                     /locus_tag="VFG22_15360"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HET6417674.1"
                     /translation="MSGSTFGQAFRVTTAGESHGPANLVIIDGCPPGLPLSEEDIIPD
                     LERRRPGQSKIVTQRKEPDAPEILSGVFEGQTTGTPIAIIVRNKDQRSRDYGNIKDVY
                     RPGHADYTFDAKFGRRDYRGGGRSSARETVARVAAGAVAKKLISEAFGGEVLGYVTQV
                     GDIRATVPAKVTLEQVEWLPDGEPNIVRCPDPVAAGQMVKLIESVRKDQDSIGGVAEI
                     VARNVPA"
     gene            complement(681..1454)
                     /locus_tag="VFG22_15365"
     CDS             complement(681..1454)
                     /locus_tag="VFG22_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HET6417675.1"
                     /translation="MAESHGQHGSTHAGSPTIDTRERGAPKDGEPQFMDRRLFMQLLV
                     FQVPTGASIAVNQEALMSALKDANIPAVVYADANDPRGVALLTFGEDPALFVDAVRPL
                     FEAEPLRHWTLRPELTMIGRTYSSGYEQNLEFWLLDRPRQTVLNPEWNWAVWYPLRRT
                     GAFEQLEGREKGGILREHATIGRAYGDQDLAHDVRLACHGLDANDNEFLIGLIGSELH
                     PLSHVVQSMRGTVQTSKYIQQMGPFFVGRAIGRNAGPQK"
     gene            1513..2259
                     /locus_tag="VFG22_15370"
     CDS             1513..2259
                     /locus_tag="VFG22_15370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417676.1"
                     /translation="MMRAFKILAVLLILGLLGLMVVGYSLNEPRPEGKPGPAADALAR
                     SMEAAVDKEAWDRTGAVRWSFFERHHYVWDRNRGLVELRWGDSRALFRTADQTGRIWR
                     GGTEQTGEDAKEGLSAAYAYWINDSFWLNPVVKFFDPGVERTLVKLDDSRDALLVSYS
                     SGGVTPGDAYLWIPGPDGLPEAWRMWVQIIPIGGIETSWEGWTELSTGAKISTQHEGW
                     GRTMTFISDVQGAEDVEALGVEPGLFDPLL"
     gene            complement(2256..3089)
                     /locus_tag="VFG22_15375"
     CDS             complement(2256..3089)
                     /locus_tag="VFG22_15375"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET6417677.1"
                     /translation="MLVGTLFFSVMSVLAKLAGERLPTMELVLARVIVTLVMSCWAIR
                     SLGIYPWGQNKKLLLVRGFAGFMGLSCYFYAINHLPLADATVIQFCNPMLAALIAVFA
                     LKERLRGLDLLATLFSMTGVVLVAQPTFLFARGAPLDPVAVTIGVVGAIFSAIAYVVI
                     RRLGSTEHHMVVVFYFPLVTGPASIPLLAYEGVVLPEGIEWLLLLGIGVAAQLGQIEI
                     TKGFKLETAGRASSVTYIQIVLAYTWGILLFGEYPNAISILGAVLVVLGVFSVTRRGM
                     P"
     gene            3196..3771
                     /locus_tag="VFG22_15380"
     CDS             3196..3771
                     /locus_tag="VFG22_15380"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HET6417678.1"
                     /translation="MRTLTRSFAQGLLVLAPVGITIWVVYFTVTTLDGWLGTSIPGLG
                     IVIAAAGITLIGYLAGNVVGTKLISWLELGLQRVPLIRILYNSLRDLFGAFVGSKRKF
                     DKPVAVELNKHGLKVLGFLTNERFDDPQLAGHVSVYLPESYNFAGNLIVVPKERVHPL
                     DADGAEFMAFIMSGGVTDMNAARTVVDRQRP"
     gene            3830..4294
                     /locus_tag="VFG22_15385"
     CDS             3830..4294
                     /locus_tag="VFG22_15385"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HET6417679.1"
                     /translation="MAAVIFKSPHDLKGSVGKHLGYSDWLTMEQDRVDKFADATEDHQ
                     WIHVDVERAKSGPFGATIAHGYLTLSLVSAFMTQIFEVRGFEFAVNYGTNKVRFPAPV
                     KVGSRIRGGAEVISVEESKGGILSVVRVTVEIEGEDRPACIAEPVSLYFPKK"
     gene            4291..4803
                     /locus_tag="VFG22_15390"
     CDS             4291..4803
                     /locus_tag="VFG22_15390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417680.1"
                     /translation="MNRGTWRCRAGINIPAMSEHTLGPSFIPLPSRFDGVSYFAVTGH
                     EHQWGTNVYVEVAESADAPGMPVYDIPTFLWDEPETVTYNDDPFTAPTAVASGSPATG
                     KPERQSRLFRHGSRRRDVLSLGLLLPEPQGSGLRQRALRLQLGLRKTCVQPARSPHAL
                     KLVHFPAKKW"
     gene            4865..6574
                     /locus_tag="VFG22_15395"
     CDS             4865..6574
                     /locus_tag="VFG22_15395"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET6417681.1"
                     /translation="MNGKSPRAFNRGWVAILLLATGVSSSAAAQNTKIDLNQFRPAEL
                     STDGFAASTPDGQGHLKFGFMVWLDYSDDPLTIENAAGGRTEIVEQQLTGHLMLNLGL
                     WDHLVLFVDLPYHFMIDSSGPGSNLLKNQNLGDLYFGARGNLYGTRDDVFQVGLQATM
                     TINTASLASSGQTFAGQADTKPYLGGWFELLLNFNAGDVVRIPIQVGYKLGTQGQNVV
                     PGELFVGNEFTYGGGLMFMMGQDHFMISAEAFGRTAANSTVNFWTKNETPVEVLGGFK
                     WLPEFGFTLGVGGSAGVTDGYGAPDWRVFGMLGYTMPADDEKAPDADGDKIPDELDNC
                     PTEAEDFDGFEDEDGCPDLDNDGDGIADADDKCPNEPEDMDGFEDEDGCPDFDNDNDG
                     ILDVDDQCPNDAGSAANNGCPDPDRDGDGVPDRIDNCPDEPGTVENQGCQAKQLVVIG
                     DDQLEILEKVYFQSGSAKLQKRSWALLDQVAAVLIAHPEIKQIRVEGHSDKTGSLKYN
                     MILSKKRADTVVRYLVGRGHVNRSRLVARGFGPNKPLVPNATTKEEEAMNRRVEFHIV
                     TKE"
     gene            complement(6884..8113)
                     /locus_tag="VFG22_15400"
     CDS             complement(6884..8113)
                     /locus_tag="VFG22_15400"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HET6417682.1"
                     /translation="MSWITEKAHAELQRVRDAQAKQIYPYFRPFESGGLHTTINGKPI
                     VNFSSNDYLGLTNHPKVKEAAKAAVDQFACGLSSSRVQATATLHVELEERLAGWFGFE
                     KSLIFTTGYQAMVGTIMSLADKDTTIVLDNLSHACILDGTFLAAGTPMRAPEVRFFNH
                     NSAKALDRVLRKRDRKNALVIIEGIYSLDGDEAKIQEFIEVCAQHENVAIVCDDAHGT
                     GTLGAGGRGILEKYGLQGQIPVLISTFSKTFGGIGGILLGEADVVDFIKHNARSFLFS
                     ASLPVPIVAAASTILDMLESEGEALVSELHEKAAYMRKGLTDIGFDLGESNSHIMPIM
                     IRDETKAMFTHVALLENGVLMVPIMYPAVKEGQERLRLNVTRGHTYEDMDRALDLLRT
                     YASSFSLVDDSPKAAAP"
     gene            8231..8470
                     /locus_tag="VFG22_15405"
     CDS             8231..8470
                     /locus_tag="VFG22_15405"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET6417683.1"
                     /translation="MSDELKDELRRIITEVTEVEDIPEDTAFADLGIDSMMAIEIVAD
                     VEKEYEITIGEDELNELVNLRAVYDKVREKLGEAD"
     gene            complement(8485..9378)
                     /locus_tag="VFG22_15410"
     CDS             complement(8485..9378)
                     /locus_tag="VFG22_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HET6417684.1"
                     /translation="MSGREPKRHQDLEDVIDVITSSYDSEEEINNLDSAALPNKRAVI
                     TAFNHIMPVLYMGFYSTRALNRTNLRYAISEHLYPAQDILVEQIDRAVRYQERMGQLS
                     EQKPEGWSEDVVLRLFENIPALRRVLNTDVRAAYDGDPAASSIEEVVFSYPSVQAITA
                     YRVAHELQLRDVPMIPRIITEHAHSKSGIDINPGAQIGERFFIDHGTGVVIGATAVIG
                     RNVKLYQGVTLGALSVSRGDANGNRGSKRHPTIEDDVTIYAGASIHGGDTVIGKGSVI
                     GGNVWLTKSVPPGSKLFGRAK"
     gene            complement(9428..10408)
                     /locus_tag="VFG22_15415"
     CDS             complement(9428..10408)
                     /locus_tag="VFG22_15415"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET6417685.1"
                     /translation="MIALVTGGGGRLGNVLVRRLVEKGQRVRVLEPGGVPESLAGLDI
                     EFQSGSVLDSGDVDRATDGVDVVFHLAAKIDLSPRKDPMMYRINVDGTRKVVDACLSR
                     GLRLVHTSSHHAVVREPLDQPLTEDKPLALDEKCQYHRSKAIGETIVLDACERGLDAV
                     IVNPGSMIGPHDYEPSMIGAALIDMYFGRVPVLLELLSDYVDVRDVADGMIAAAERGR
                     RGERYFLTGDVIPIMEMVSLYGELTGAKVPTRALPLWVGWALLPFALAGSAVTKKPAF
                     ITADMLRASVSNEVVSHEKASRELGYTIRSLRESLGDAVGWYRERGWLGA"
     gene            complement(10441..12090)
                     /gene="pgm"
                     /locus_tag="VFG22_15420"
     CDS             complement(10441..12090)
                     /gene="pgm"
                     /locus_tag="VFG22_15420"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HET6417686.1"
                     /translation="MTAHPLAGQPAPKGVLVDVPKLIAAYYEGKPDPNEPAHRVSFGT
                     SGHRGSSFRLSFNEDHILATTQAICDYRVAEGIDGPLFLGIDTHALSVPAARTAIEVL
                     SANGVETRIAAEGEFTPTPAVSFAILGHNRNSAKDADGIVVTPSHNPPEDGGFKYNPP
                     HGGPADTEVTSWIEDRANRLLAARLKDVSRNTYEAALRAETTQVYDFITPYVDGLETV
                     LDMRTIASAGLRIGVDPMGGAGIAYWPRIAERYGLRLDIVNQTIDPTFSFMTLDHDGK
                     IRMDCSSPYAMANLVDLRNQYDIAFANDTDFDRHGIVTSSWGLLNPNHYLSVAISYLF
                     TERNWNERAAVGKTLVSSAMIDRVAKDLRRRLAEVPVGFKWFVDGLIDGSYGFGGEES
                     AGASFLRKDGTVWTTDKDGILLNLLAAEITATTGRDPGECYQSLTTQFGAPIYERTDA
                     PATPKEKAALKGMSESDLDASELAGEPIKQVLTKAPENGAAIGGLKVTTENGWFAARP
                     SGTEDVYKIYAESFVGREHLAAIQGEAKDLIGRVFDKDASA"
     gene            complement(12123..12599)
                     /gene="bfr"
                     /locus_tag="VFG22_15425"
     CDS             complement(12123..12599)
                     /gene="bfr"
                     /locus_tag="VFG22_15425"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HET6417687.1"
                     /translation="MKGNDEVIQVLNEVLTSELTAINQYFIHSKMCEDWGFEKLAAKK
                     RAESIEEMKHADIVIARILYLEGVPNMQRYFPVKVGEDAVEQHRLDLAVEYDAVKRLN
                     AGISLCREKGDNGTRDLLETILKDEEEGIDWLEAQLHLVETVGRERYLSEQMGGGN"
     gene            complement(12970..13992)
                     /gene="bioB"
                     /locus_tag="VFG22_15430"
     CDS             complement(12970..13992)
                     /gene="bioB"
                     /locus_tag="VFG22_15430"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HET6417688.1"
                     /translation="MIDVRNDWTKAEARALHDLPLTDLLFRAQEVHRATQPRDAVQLC
                     SLLSIKTGACQEDCSYCPQSSKYDTGVEAERLMDVADVLDKARQAKEAGASRFCMGAA
                     WRSPKKGSRQFQQVLEMVRGVRELGLEACATLGMLDEEQALELKDAGLTAYNHNLDTS
                     EEFYGEIITTRTYKDRLDTIGRVAAAGISVCAGGIIGMGETIDDRVGMLVTLANLSPQ
                     PESVPVNALVAVPGTPLSEQTPVDSLELVRMCATARIMMPGARVRLSAGRAALSREAQ
                     LLCFMAGANSIFFGDKLLTTGNPDHESDLTLLRDAGLAALAPEGAGQGPASESGRGDA
                     VSGAAE"
     gene            14087..15091
                     /locus_tag="VFG22_15435"
     CDS             14087..15091
                     /locus_tag="VFG22_15435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417689.1"
                     /translation="MAAITSDEHERSAYLAAAPPLSWMAGAAALADMVINRLLVPLGS
                     DAWSAATLLELSRWGGFARNLSVVSALVALSFCLVSLSSPRTGVSFSGRAAISSFGWV
                     LVPIIALMTFLPRALTRVELVLVVAGLTNALILLLVLAGVQWRATRPVAVSLMLTMVA
                     ALSGMLSMVVTAVGGRAYWEHTERLSNAFRWSGELAYLAVPIAIGLAISIPWREARGK
                     AAVMLSALTAAFVATSMVLSKRAARLDLPELVYGAMRLDLLPDDDFVLYAIPLGVGSA
                     VTMAAILSRDPIRRQLGAALLLFLSAGYAPRTPSALLISVLGVALLARAGIALEQRVR
                     "
     gene            complement(15111..16319)
                     /locus_tag="VFG22_15440"
     CDS             complement(15111..16319)
                     /locus_tag="VFG22_15440"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET6417690.1"
                     /translation="MSERIPVAQRLSAVKPSATVAVAQRARELKEQGIDVLSFSVGEP
                     DFGTPAHIGEAAKKAIDSGATRYTAVGGTVELRKAICEASAKRRAGVTYGPSEVLVSV
                     GAKHTLFNLALALYDEGDEILIPAPYWVSYPEQVLLAGAKPVIVETSEENGFSMTPEA
                     LRAAITPKTKALILCSPSNPTGAAYTGAQLRALADVAAEHNFWIIVDEIYGQLVYGGF
                     DQKSIIEVAPELKDRIIIVDGVSKTFAMTGWRIGWMLAPEYVVKACEKVQGQATTNPS
                     SIAQSAAVAALRGPWGPMEEMRKAFEERRSIIVDGLNAIPGISCSMPEGAFYAFANVQ
                     GLIGKQGGGKTLQSDLDVSGYLLEEARCAVVPGTAFGAPGFVRISYAASNEMIREGLR
                     RIGEAVSKLE"
     gene            complement(16351..16962)
                     /locus_tag="VFG22_15445"
     CDS             complement(16351..16962)
                     /locus_tag="VFG22_15445"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HET6417691.1"
                     /translation="MRSKASGLVLLGLWGLLVLAAQGCSKSAADVPVLGELPEFSLID
                     QNEQAFTRESMAGNLWVSAFVFTHCRSTCPRITAHMKGLQSRLSDVAGASFVSVSVDP
                     RNDTPEVIKAYMVKNDLDERNWRFVTGSEEAIRHVVVEGFRVPIGDEDSRAAGADDIM
                     HSNSFVLVDKEARVRGYYRANNDGIGELERDLRALAAASDAKR"
     gene            complement(16952..17833)
                     /gene="cyoE"
                     /locus_tag="VFG22_15450"
     CDS             complement(16952..17833)
                     /gene="cyoE"
                     /locus_tag="VFG22_15450"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HET6417692.1"
                     /translation="MTVATHPATMILTVRDLIALTKPRITLMVLVTAAGGMWMAPGSL
                     SIRSMAVMLLTTGMVVGAANTLNCYLERDTDRLMARTANRPLPDRRLEPSWALTLGVL
                     MGLFAVPTLVLAVNPVTGLLGFVALASYVAIYTPMKQYTPAALFVGAVPGALPPLMGW
                     TAVTGRIDVPGLVLFGVLFFWQIPHFIAISIFRQEEYERAGLKVLPSVHGIRSAKLQS
                     VIYAGALWLVSIMLVPYGLAGTIYLGAALVLGAYFFWKALLGFKAEDDKVWAKQFFLA
                     SLIYLTALFAALIVDAL"
     gene            complement(17932..18993)
                     /locus_tag="VFG22_15455"
     CDS             complement(17932..18993)
                     /locus_tag="VFG22_15455"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6417693.1"
                     /translation="MSDPYLNKEVAGQFRIVEKIGKGGMGAVYKAEQPDMNRYVAIKI
                     LHSRYLSRSDLVSRFRREARAMSQLSHPNTARVFLYGQLDDGACYFVMEHLVGRNLAQ
                     IVRAEGAMKPKRAMRIMIQVCGALEEAHRAGIVHRDLKPENIFVTSQGGIRDFPKVLD
                     FGLAKVTERQMRPGSMVLTQQGMVFGTPEFMSPEQAHGKTLDARSDIYSLGVILYELL
                     TGKLPFDANQPIEFIQLHVNATPIPLSQRRPETDFPPGLEDVVMAALAKKPEDRYASA
                     AEFAAALENVLQVEAGEAEAISLPTPIPAAPVRDPAPAIEPLRGEARPKTPWIMLGVG
                     VALAVAGILALLAAFGPGS"
     gene            complement(18990..19922)
                     /locus_tag="VFG22_15460"
     CDS             complement(18990..19922)
                     /locus_tag="VFG22_15460"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HET6417694.1"
                     /translation="MRSPNRFAVAASALLVYTVAVVLWGAYVRATLSGDGCGAHWPLC
                     NGVALPVAPSLKTIVELTHRVTSGLCWLWTLLMWIAALRIFERGHATRSAAGWSLFFM
                     STEALVGAGLVLFRMVADNPSTARAAWMSAHLVNTFLLLSALTWMAFSAFQKQGTRFP
                     GDRRSRLLLGGSAAVVLLVGVSGAVAALGDTLFPASSLADGLLEDLSPTSHLFLRLRL
                     WHPVIAILGSSYLIWLASVFLRRTPDRWVRLLALALGALVLIQIAVGLINIALLAPVA
                     TQLVHLLIADATWICLTLLALSSPTPTSDANLVS"
     gene            complement(19944..20540)
                     /locus_tag="VFG22_15465"
     CDS             complement(19944..20540)
                     /locus_tag="VFG22_15465"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HET6417695.1"
                     /translation="MSHYLTALFAAILVATTFGCDKSKLYEDLTDDSTWETSVGEEPI
                     ESGAVEDPPKRNDAWEEKHDPMDGAAPAEESKPESSLEQSYPRVSTGVIAREALDPVL
                     DGGLGRFLQNVETEPAFHKGAFVGFRILSLFPGEPEFASLDLRPGDTVTRVNGKPIER
                     PEQAIAVWEDLRTASKLVVDYRRDGEPHTLRFTIVDEG"
     gene            complement(20537..21190)
                     /gene="tmk"
                     /locus_tag="VFG22_15470"
     CDS             complement(20537..21190)
                     /gene="tmk"
                     /locus_tag="VFG22_15470"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HET6417696.1"
                     /translation="MIEGHFIVLEGIDGSGTTTQSAILADRLSEKGLPVHTTHEPSDG
                     PIGVLIRQILTGRVVVPGRHGVRPPNWGTMATLFASDRLDHLQAEIIPNLTDGVTVIC
                     DRYDHSSVAYQTVSSGSGVEVSNWVRELNRRARRPDLTLVLDVDPAQAARRRGTRSGS
                     PELYEVDEMQAELAKFYAKIDELFPDDTIHHIDANQSVDAVATAIWSQVTALRGESA"
     gene            complement(21187..>22934)
                     /locus_tag="VFG22_15475"
     CDS             complement(21187..>22934)
                     /locus_tag="VFG22_15475"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET6417697.1"
                     /translation="TSGAAIRIDGEPSGTVPVRTTLSPGRHLVQVGKRGFVTFSKWVD
                     LIGGQVLTIPVVLQRQAAKTGSLLVTADVTGIPVFIDGQQRGATPLVVDGLSEGEHLV
                     EIRSPGEGYQPFFEKVTIRASERTTVEATIRIAPDLGSLRVITNVPGAIISLDGIDIG
                     VAPAAKGGLTPGEHVVMARASGYQAVDQAVTVIAGRERVVSLRFTTASTDVARILVRA
                     SVPEATVTIDGEDYGNPPIAIEPAEFGTHSIVVRAPGFREVRRTCSVGPTRNCDVYAE
                     LNPLGVPVRVEANVSQAQLYVDGELLGPVPWEGDLAAGTHQLEVRAAGYGNHVEQIRL
                     QKSPRIRLIQVILSSAAAPANQSKNDKESERSDPLRNTVTHAGAPNPASVATLDFSTG
                     WPWLASVGLRAGLKPFLDVGIYVATFGRLTDFWGSTKAGWRVSRYFSVGGDLRVGGGV
                     GPSHRDVDGGSHPTNNFFISLDAILSLHFPPRGAFSVWIAGDFNSDRWDFEGTNSNVY
                     VTSSRQNLARARLGGSVEIVVTQKWNVWLQFEGIVAGDRRRVLGDIFGGGHPDSKVYG
                     QFGATFKFGGAKEGS"
BASE COUNT         4397 a         7074 c         7102 g         4361 t
ORIGIN      
        1 gccggaacgt tacgtgcgac gatttcagcg actccgccta tcgagtcctg atcttttcgc
       61 acactctcga tgagcttgac catctgcccg gctgcgaccg ggtcggggca acgaacgatg
      121 ttgggctcgc cgtctgggag ccattcgacc tgctcgagtg tgacctttgc agggaccgtt
      181 gctcgaatgt cgcctacttg ggtcacgtaa ccgagcacct cgccaccgaa tgcctcggag
      241 atcagtttct tcgcgaccgc gccggcggca acgcgcgcga ccgtttctct ggcgctgctc
      301 cgccctccgc cacggtagtc acggcgcccg aacttcgcgt cgaatgtgta gtcggcgtgc
      361 ccgggccggt acacgtcctt gatgttgccg tagtctcgcg agcgctggtc cttgttacga
      421 acgatgatcg cgatgggcgt tcccgtggtc tgcccctcga acacgccgga gagaatttcg
      481 ggagcgtcgg gctcttttcg ttgcgtgacg atcttgcttt ggcccggcct gcgtcgctcg
      541 aggtcgggaa tgatgtcttc ttccgacaag ggaagacccg gcggacaacc gtcgatgatg
      601 actagattgg ctgggccatg gctctcgccc gcggtggtca cgcggaaggc ctggccgaac
      661 gtactacctg acatggcggg tcacttctgt gggcctgcgt tacggccgat cgctcgcccg
      721 acgaagaatg gccccatctg ttggatgtac ttcgaggtct gcacggtacc ccgcatcgac
      781 tgcaccacat gagagagcgg atgcagctcc gagccgatga gtccgatcag gaactcgttg
      841 tcgttcgcgt cgagaccgtg gcaggcgagg cggacgtcgt gggcaaggtc ttgatcgccg
      901 tatgcgcggc cgatcgtagc gtgttcccga agaatgccac ccttctctct tccttcgagt
      961 tgctcgaagg ctccggtccg acgcagtgga taccaaacgg cccagttcca ctccgggttg
     1021 agcacggtct gtcgcgggcg gtcgagcagc cagaactcga gattctgctc gtacccgctg
     1081 ctgtacgtgc gaccgatcat cgtgagctcc gggcgcagcg tccagtgccg aagaggctcc
     1141 gcctcgaaca gcgggcgaac cgcgtccacg aaaagtgccg gatcttcgcc aaaagtcaga
     1201 agcgcaacgc cgcgagggtc gttggcgtcg gcgtagacta cggccgggat gtttgcgtct
     1261 tttagcgcag acatcagagc ctcctggttg acggcgatgg acgcacccgt gggcacctgg
     1321 aaaaccagca gctgcatgaa caagcggcga tccatgaact gtggctctcc gtccttggga
     1381 gcaccacgct cgcgtgtgtc gatcgtgggc gatccggcgt gcgtgctgcc atgttgtccg
     1441 tgcgattcag ccatgaccgg actcatagcg cttttgtcgc ctgtctgcac agtgtaggct
     1501 caactgactt caatgatgcg cgcgttcaag atcttggcgg tgctcctgat tctgggcctt
     1561 ctcggcctca tggtcgtcgg ttactcgctc aacgaacccc ggcccgaagg aaaacccggg
     1621 cctgcggccg atgctctcgc tcgatcgatg gaagccgccg tcgacaagga ggcctgggac
     1681 cgcaccggtg ccgttcgctg gtccttcttc gagcggcatc attacgtctg ggatcggaat
     1741 cgcggtctgg tggagcttcg ctggggcgac tcacgggccc tcttccgcac ggcggatcaa
     1801 acggggcgca tctggcgcgg aggcaccgag cagacgggcg aagacgcgaa agaaggtctg
     1861 agtgcggcgt acgcgtactg gatcaacgac tccttttggc tcaacccggt cgtgaagttc
     1921 ttcgatccgg gcgtcgagcg gacgctggtg aagctcgacg atagccggga cgctctattg
     1981 gtcagctaca gctcgggggg tgtcacacca ggtgatgcgt acctctggat tccaggtccc
     2041 gacggccttc ccgaggcctg gcgcatgtgg gtccagatca ttccgatcgg tggcatcgaa
     2101 accagctggg agggttggac cgagctcagc acgggcgcga agatctcaac ccagcacgag
     2161 ggctggggac gcacgatgac gttcatcagt gatgtacagg gcgcggaaga tgtggaagcg
     2221 ctcggtgtgg aacccggatt gttcgatccg cttctctagg gcatcccgcg gcgcgtgacg
     2281 ctaaacacgc cgagcacgac caggaccgca ccgaggatgc tgatcgcgtt gggatactcg
     2341 ccgaaaagca gaatgcccca agtgtaagcc agaacgatct ggatgtaggt caccgatgat
     2401 gcgcggcccg cggtttcgag cttgaatccc ttggtaatct cgatctgacc gagttgggct
     2461 gccacgccga tacccagcaa gagaagccac tcgattcctt cgggaaggac cacgccttcg
     2521 taggccaaga gcggtatgga cgctgggccc gtgacgagtg gaaagtagaa cacgacgacc
     2581 atgtgatgct cggtagagcc caagcgccgg atcacgacgt acgcaatggc cgaaaagatc
     2641 gcgcctacca ctccgatggt cacagccacc ggatccaggg gcgcgcctcg tgcaaacaag
     2701 aacgtgggct gtgcgactaa gacgaccccg gtcatgctga acagcgtcgc gagcaagtcc
     2761 aggccgcgaa gcctttcctt gagggcgaag accgcgatca gcgcagcgag catcgggttg
     2821 cagaattgaa tcaccgtggc gtctgcgagt ggcagatggt tgatcgcgta gaagtaacaa
     2881 ctgagaccca tgaagccggc aaagccgcgc actagaagca gcttcttatt ctgaccccag
     2941 ggatagatgc cgaggcttct gatcgcccag caactcatga cgagtgtgac gatgacgcgc
     3001 gcgagcacca gctccatcgt cggcagccgt tcgccggcga gcttcgccaa aacgctcatc
     3061 acgctgaaaa acagagtgcc caccaacatg tggcgaatcc cgagggaaag gccttgccaa
     3121 cgtggaaaag ggagcggcac tcagcgcgtt ctggctggta atgcgctgga cgcaagcggg
     3181 tgttagtaga tacgcatgag aacgctcacg agaagcttcg cgcaggggct cctggtcctt
     3241 gcaccggtcg gcatcactat ctgggtggtc tacttcacgg tcaccacgct cgatggctgg
     3301 ctcggaacgt ccattccggg gctcggcatc gtcatcgccg cagccggaat caccctgatc
     3361 ggctacctag ctggcaacgt agtgggcacg aagctcattt cgtggttgga gctaggcttg
     3421 cagcgggttc ctctgattcg aattctctac aactcgttgc gagacctctt tggcgcgttc
     3481 gtagggagca agcgcaagtt cgacaagccg gtcgcggtag agctgaacaa gcacggtctg
     3541 aaagtacttg ggttcctcac gaacgaacgc ttcgacgacc cgcagctcgc cggccacgtt
     3601 tcggtgtact tgcccgagtc gtacaacttt gcgggcaatc tgatcgtcgt ccccaaggag
     3661 cgcgtccacc ccctggatgc ggacggcgct gagttcatgg ccttcatcat gtcgggcgga
     3721 gtcaccgaca tgaacgccgc caggactgtc gtcgatcgcc aacgacccta gaaacgagac
     3781 aactggcgct ggcagcgaga gagactagta tcgtcgaccg gagcaagtga tggcagcagt
     3841 aattttcaaa tccccacacg atctcaaagg ctccgtcggc aagcatctcg gatacagtga
     3901 ctggctgacg atggagcagg accgggtcga caagttcgca gacgcgaccg aagaccatca
     3961 gtggattcac gtcgacgtgg agcgggcgaa gtcagggcca tttggtgcga caattgcgca
     4021 cggatacctg acgctgtcgc tcgtgagcgc gttcatgacc cagatcttcg aagtgcgcgg
     4081 cttcgagttc gcggtgaact acggcaccaa caaggtccgc ttccccgctc cagtgaaggt
     4141 cggctcgcgc attcgcggag gggccgaggt catctcggtc gaggagtcga agggcggtat
     4201 cctctcggta gtccgcgtca ccgtggaaat tgagggcgaa gaccgccccg cctgcatcgc
     4261 agagccggtt agtctgtatt tcccgaagaa gtgaaccggg ggacctggcg ctgccgcgcc
     4321 ggcatcaaca tcccggcgat gagtgagcac acgcttggtc cgagcttcat tccgctccca
     4381 tctcgcttcg acggagtgag ctatttcgcg gtcaccggac atgagcatca gtggggcacg
     4441 aacgtctacg ttgaggtcgc ggagagtgca gatgcgcccg ggatgcccgt ctacgatatc
     4501 cccaccttcc tgtgggacga gccggagacg gtcacctaca acgacgaccc gtttaccgcc
     4561 ccgacggcgg tggcttcagg atcacctgcg actggaaaac cggagcgaca gtcccgtcta
     4621 tttcggcacg ggagtcgacg acgagatgtg ctttctctgg gcctactact tcccgagccg
     4681 cagggctctg gtctgcgcca acgggctctt cggctccaac taggcttgcg caaaacctgc
     4741 gtccaaccag ctcggtcgcc ccatgcgctc aagctagtcc actttccagc caagaagtgg
     4801 tagacacgta ggcgctcgcg gggcccgaga gtgccccgga tacggaaatg ggaggaagag
     4861 cgtgatgaac ggtaagagcc caagggcatt caatcggggt tgggttgcga tcctgttgct
     4921 ggcgacgggg gtctcctcca gcgcggctgc gcagaacacc aagatcgacc tcaaccagtt
     4981 tcgacctgct gagctctcga cggacggctt cgccgccagc acaccggacg gccaaggaca
     5041 cctcaagttc ggcttcatgg tctggctcga ctattcggac gacccactca ccatcgagaa
     5101 cgccgcagga ggcagaaccg agatcgtcga gcagcagctc acggggcatc tcatgttgaa
     5161 tctcggcttg tgggaccatt tggttctgtt tgtcgacctg ccttaccact tcatgatcga
     5221 ttcctcgggt ccgggaagca acctcctcaa gaaccagaat ctcggcgacc tctacttcgg
     5281 cgccaggggc aacctctacg ggacgcgcga cgacgtcttc caggtcggtc ttcaagcgac
     5341 gatgacgatc aacaccgctt cgctggcaag cagcgggcag accttcgctg ggcaagccga
     5401 cacgaaaccg tacctcggag gctggttcga gctcctactc aacttcaatg ccggcgacgt
     5461 cgtccgtatc ccgattcagg tcggctacaa gctcggtacg caggggcaga acgtcgttcc
     5521 tggagagctc ttcgtcggaa acgagttcac ctacggcggc ggcctgatgt tcatgatggg
     5581 acaggaccat ttcatgatct cggcggaggc cttcggccga accgcggcaa acagcaccgt
     5641 gaatttctgg acgaaaaacg agactccggt cgaagtgctc ggcggcttca agtggcttcc
     5701 ggagttcggg ttcacactcg gcgtcggcgg cagtgcgggg gtcacggacg gctatggagc
     5761 gcctgattgg cgcgtgttcg ggatgctcgg atacaccatg cctgccgacg acgagaaggc
     5821 gccggacgcc gatggcgaca agattcccga cgagctcgac aattgcccga ccgaagcgga
     5881 agacttcgac ggcttcgaag acgaagacgg ttgcccggac ctcgacaacg acggggatgg
     5941 catcgccgac gcggacgaca agtgtcccaa cgagcctgag gacatggatg gcttcgagga
     6001 cgaggatggc tgtcccgact tcgacaacga caacgacgga atcctcgacg tcgacgatca
     6061 gtgccccaac gacgctggct cagcggcaaa caacggctgt ccagacccgg atcgcgatgg
     6121 cgacggtgtt ccggatcgca tcgacaattg cccggatgag cctggcacgg tcgagaatca
     6181 aggctgtcag gcgaagcagc tcgtcgtgat cggcgacgac cagctcgaga ttctcgagaa
     6241 ggtctacttc cagtctggca gcgccaagct gcaaaagcgc tcctgggcgc tgctcgacca
     6301 ggtcgctgcg gtgctgatcg cccacccgga aatcaagcag attcgggtcg aagggcacag
     6361 tgataagaca ggaagcctca agtacaacat gatcctgtcg aagaagcgcg ccgacacagt
     6421 ggtccgctac ctcgtcggca ggggtcacgt gaacaggtcg cgtctggtcg cgaggggctt
     6481 cggtccaaac aagccgctcg tgcccaacgc cacgacgaaa gaagaagagg cgatgaaccg
     6541 acgcgtcgag ttccatatcg tcacgaagga atagaccggg gagcaatcct cagtcgtcac
     6601 ggctgacggg ctccgagcta acgctcgggg cccgtcgcgt ttgaggacac cggcgctgac
     6661 accggcgctg acaccggcac tgacactgcc gctgacactg ccgctgccgc tgccgctggc
     6721 actgacatgc ggagggggat ggctcgggat gggcagcgca gcgattgggt ccagcctagc
     6781 cgtaggtcgt cttagggaac tgggggcgag tgggtcccaa tcgcgagcac gcccgtcgcg
     6841 agccgtcctc cggtgacgca gtcgcgccaa gtgactggcg ctgtcagggg gcggcggctt
     6901 ttggcgaatc atcgacgagc gaaaacgagc ttgcgtatgt tcgcaagagg tccagcgccc
     6961 ggtccatgtc ctcgtacgtg tgcccgcggg tgacattgag ccggaggcgt tcctggcctt
     7021 ccttcacggc gggatacatg atcggaacca tcaggacgcc gttctccaag agcgcgacgt
     7081 gggtgaacat cgccttggtc tcatcacgaa tcatgatggg catgatgtgc gagttgctct
     7141 cgccaaggtc gaagccaatg tcggtgagcc ccttgcgcat gtaggccgcc ttttcgtgaa
     7201 gctcgctgac cagcgcctct ccttcggact cgagcatgtc gagaatcgtg ctggccgcgg
     7261 ccacgatcgg cacgggcagg gacgcgctaa acaagaatga acgtgcgttg tgcttgatga
     7321 agtccaccac gtcggcttcg ccgagcaaga ttccgccgat gcccccaaag gtcttcgaga
     7381 aggtgctgat gagcaccggg atctgacctt gaagaccgta cttctcgagg attcccctcc
     7441 caccagcgcc caaggtgccc gtgccgtgcg cgtcgtcgca gacgatcgcg acgttctcgt
     7501 gctgcgcgca gacttcgatg aattcctgaa tcttcgcctc gtcgccatcg agagaataga
     7561 tgccttcgat aatcacgagg gcgttctttc gatcgcgctt tcgtaggacc cggtcgagtg
     7621 ccttggcgct attgtggttg aagaatcgga cttccggcgc ccgcatcggc gttcccgccg
     7681 ccaagaacgt gccgtcgaga atgcaggcgt ggctgaggtt gtcgagaacg atcgtggtat
     7741 ccttgtcggc cagcgacatg atcgttccga ccatcgcctg gtatccggtg gtgaagatca
     7801 aagacttctc gaatccgaac cacccagcca agcgctcctc gagctctacg tggagcgtcg
     7861 cggtcgcctg aactctcgac gagctcagtc cgcacgcgaa ctgatccacc gccgcctttg
     7921 ccgcctcttt aaccttggga tgattggtca gcccgaggta atcattcgag ctgaagttga
     7981 cgatcggctt tccgttgatc gtcgtgtgca gcccgccgga ctcgaagggg cggaagtagg
     8041 ggtagatctg cttggcctgt gcatcgcgga ctcgttgcag ctccgcgtgc gccttttctg
     8101 taatccagct catatgcttt gcgggcccta acgtaccgta atttccagtg gataccacat
     8161 tcgaaacagg ccaatcggcc agttttcaaa cgcttgacgc tcatggtgaa cccaagcata
     8221 tacgggcggc atgtcggatg agctgaaaga cgagctgcgg cggatcatca ccgaggtgac
     8281 agaagtcgag gatatccccg aggatacggc attcgcggac ctggggatcg acagcatgat
     8341 ggcgatcgaa atcgtggccg acgtcgagaa ggaatacgag atcacgatcg gcgaagatga
     8401 gctcaacgag ctggtcaacc tcagggccgt ctacgacaaa gtcagagaaa agctcgggga
     8461 agccgactga ccgcttcgga agcgctactt cgcgcggccg aagagcttcg aacctggtgg
     8521 cacggatttg gtgagccaga cattgccgcc gatgaccgag cccttgccga tcacggtgtc
     8581 tcctccgtga atcgatgcgc ccgcgtagat cgtgacatcg tcctcgatcg tggggtgccg
     8641 tttgctgccg cggtttccgt tggcatcgcc gcggctgaca ctcagcgcgc ctagggtgac
     8701 gccctggtat agcttcacgt tgcggcctat caccgctgtt gcgccaatca cgacgcccgt
     8761 tccgtgatcg atgaagaagc gctcgccgat ttgggcaccg gggttgatgt cgatcccaga
     8821 cttgctgtgc gcatgctccg tgatgatgcg gggaatcatc ggcacgtctc tgagctgcaa
     8881 ctcgtgcgcg acacggtacg ccgttatcgc ctgcacactc ggatagctga agacaacctc
     8941 ttcgatgctc gaagcggccg ggtctccgtc gtacgcggcg cgcacgtcgg tgttgagcac
     9001 gcggcggagc gcggggatgt tctcgaacaa acgcagcacc acgtcctcgc tccagccttc
     9061 gggcttctgc tcggagagtt ggcccatccg ctcttggtac cgcacggccc ggtcgatctg
     9121 ttcgaccagg atgtcttgcg ccggatacag gtgctcgctg atcgcgtaac gaaggttggt
     9181 ccggtttagc gcgcgagtac tgtagaaacc catgtagagc acgggcatga tgtgattgaa
     9241 ggccgtgatc acggcgcgct tgttcggaag agcggcgctg tcgagattgt tgatctcttc
     9301 ttcgctgtca taggatgagg tgatgacgtc tatgacgtcc tcgaggtcct ggtgacgctt
     9361 gggctctctt ccgctcatga cttctgtatg tgtagcacgg cggatgcgtc aatcgcgaaa
     9421 acggttgtca tgcgccgagc caaccacgct cccggtacca gcccactgcg tcgccgagcg
     9481 actcgcggag gctgcggatc gtgtacccta gctcgcggct tgccttttca tgggaaacca
     9541 cctcgttcga gacgctggca cggagcatgt cggcggtgat gaacgctggc ttcttcgtga
     9601 ctgcgctccc cgccagggca aacggcaaga gggcccaacc cacccaaagc ggcagggcac
     9661 gcgtgggtac cttggctccg gtcagctcgc cgtagaggga gaccatctcc atgatgggaa
     9721 tgacatcacc tgtgaggaag tagcgctctc cgcggcgacc tctctccgca gcggcgatca
     9781 tcccgtcggc aacgtcgcga acgtcgacgt agtcactgag cagctcgagc aggacgggca
     9841 ctcgaccgaa atacatgtcg atcagcgccg cgcctatcat cgacggctcg tagtcatgcg
     9901 ggccgatcat cgagcccgga ttcacgatca ccgcgtcgag accgcgctcg caggcatcga
     9961 gcacgattgt ctccccgatc gctttcgagc ggtgatactg gcacttctcg tcgagggcga
    10021 gcggtttgtc ctcggtgagc ggctgatcga ggggctcgcg caccacggca tgatgcgagc
    10081 tcgtgtgcac cagccgcaag ccacgagaaa gacacgcatc gacgaccttg cgagtgccgt
    10141 cgacgttgat gcgatacatc atcgggtctt ttctggggct gaggtcgatc ttcgcggcca
    10201 agtggaagac gacgtctacg ccatccgtgg cccggtcaac gtcaccggaa tccagcaccg
    10261 aaccggactg gaactctata tccagcccgg cgagggattc gggcacgcca cctggctcca
    10321 agacacgaac ccgctggccc ttttcgacga ggcgcctcac taagacgttg ccgagacggc
    10381 cgccaccacc tgtaacgagt gcaatcacgc ggcgcagtga aacacgattg ggctaggccc
    10441 tcaagcgctc gcgtccttgt cgaatacgcg accgatgaga tccttggctt cgccttgaat
    10501 cgcagccaaa tgctcgcggc cgacaaagct ctcggcgtag atcttgtaca cgtcttcggt
    10561 cccggaaggt cgcgccgcga accagccgtt ctccgtcgtg actttcagac caccaatcgc
    10621 agccccgttt tccggtgcct tcgtgagcac ttgtttgatc ggctcgcctg caagttcgga
    10681 cgcatcgagg tccgactcgc tcattccctt gagcgctgct ttttccttgg gagtggcggg
    10741 cgcatctgtg cgctcgtaga tcggcgcgcc gaactgtgtg gtgaggctct ggtagcattc
    10801 accgggatcg cgacccgtcg tcgcggtaat ttcggccgca aggaggttga gtagaattcc
    10861 gtccttgtcc gtcgtccaaa cagtgccgtc cttgcgaagg aacgaagcac ccgcactttc
    10921 ctctccgccg aatccgtatg agccatcgat gagtccatcg acgaaccact tgaagccaac
    10981 cggaacctcg gcaagtcggc gtcgcaggtc tttggccact cgatcgatca tcgcgctgct
    11041 caccagcgtc ttgccaaccg ccgcgcgctc gttccagttg cgttcggtga acaagtagct
    11101 gatcgcgacg ctgagatagt gattgggatt gagcaacccc caactggaag tcacgatccc
    11161 gtgccggtcg aagtccgtgt cgttggcgaa agcaatgtcg tactggttac gtagatcgac
    11221 caagttcgcc atggcgtacg gcgaagagca atccatgcgg atctttccgt cgtggtcgag
    11281 cgtcatgaac gaaaaggtcg ggtcgatggt ctgattaacg atgtcgagcc gaaggccgta
    11341 gcgctctgca attcgcggcc agtacgcgat cccagcgccg cccatcgggt cgaccccaat
    11401 ccgaaggcct gcacttgcta tcgttcgcat gtcgaggacg gtctcaagtc cgtcgacgta
    11461 gggcgtgatg aagtcgtaga cttgggtcgt ctcggcacgc agcgccgcct cgtacgtgtt
    11521 gcgacttacg tccttcaggc gagccgctaa caagcgattc gcccggtctt cgatccacga
    11581 cgtgacctcg gtatccgcag ggccgccatg cggtggattg tatttgaagc cgccatcttc
    11641 aggcgggttg tgcgaaggag ttacgacgat gccatcggca tctttcgcgc tgttccggtt
    11701 gtgcccgaga attgcgaacg acaccgccgg cgtcggcgtg aactcacctt cggcagcgat
    11761 gcgtgtctcg accccgttcg ccgagagaac ctcgatggcg gtgcgtgcag ccggcacgga
    11821 aagcgcatgt gtatcgatcc ccaggaacaa aggaccatcg atcccctcgg cgaccctgta
    11881 gtcacagatg gcctgcgtcg tcgcgaggat gtgatcttcg ttgaagctga gccgaaagga
    11941 ggagccccga tgcccggatg ttccaaagct cacacggtga gccggttcgt tcgggtcggg
    12001 cttgccttcg tagtaggcag cgatgagctt cggaacgtcg acgaggacgc ccttgggcgc
    12061 gggctggcca gcaagcggat gtgcggtcat cgctgagctt ctagcagccc agcgtgcggg
    12121 agctagttcc cgccgcccat ctgctcggag aggtagcgct cacgacccac ggtttccacc
    12181 agatggagct gcgcctcgag ccagtcgatg ccctcttctt cgtccttgag gatcgtttcg
    12241 aggagatcgc gggtgccatt gtcgcccttc tcgcgacaga ggctgatccc tgcgttgaga
    12301 cgcttcacgg cgtcatattc gaccgcgagg tctagcctgt gctgttcgac cgcatcctcc
    12361 ccaactttga ccgggaagta ccgctgcatg ttgggcacgc cctcgaggta gagaatgcgt
    12421 gcgatgacga tgtccgcatg cttcatctcc tcgatcgact ccgcccgctt cttcgcggcc
    12481 agcttttcga aaccccagtc ctcacacatc ttcgagtgaa tgaaatactg gttgatggcg
    12541 gtgagctctg aggtgaggac ctcgttgagc acctggatga cttcgtcgtt gcccttcatg
    12601 gattttcctt caggcgagca tagggacctc gaacctgggt ggcaatccag caagtttgaa
    12661 gatgaaagcc gttatcagtc ggcgccctag ccttgcgccc cgttggcacc tgactggctc
    12721 tgactgccat ggtgcagctc aatcaaacga tcgatcaccg ggcgacaact cccgcaggtc
    12781 ccgccggccc cacaggccgc gcgaacttcg ctctcgctgc gcgcgccagc gtcgacgcat
    12841 cggcgaatca tgtgatcgtt aaccgcatgg cagtggcaaa ccaacatgct gtatttatag
    12901 ctgctggaac ttgcaaatca aattcaataa cgacttatct aaaaaccgta atgatttcga
    12961 gggtgaattc tactcagcgg cgccagaaac cgcgtcgccc cgaccggatt cgctggccgg
    13021 tccctgcccc gccccctctg gggcgagcgc tgcgagtccg gcgtcgcgca ggagggtcag
    13081 gtcggactca tggtcagggt ttccagtcgt gaggagcttg tcgccgaaga agatgctgtt
    13141 cgctcccgcc atgaagcaaa gcagctgcgc ctctcggctg agcgcagcac ggcccgccga
    13201 aagacggacc cgcgcgccgg gcatcatgat gcgagccgtc gcgcacatgc gcacgagctc
    13261 gagcgagtca accggggtct gttcgctcag cggcgtgcca ggcaccgcca ccaaggcgtt
    13321 caccggtacg ctctcgggct gcgggctgag gttcgcgagc gtcacgagca tgccgacgcg
    13381 atcgtcgatc gtctccccca ttccaatgat gccgccggcg cagacggaaa tcccggcggc
    13441 ggccacacgg ccgatcgtgt cgaggcgatc tttgtaggtt cgggtcgtga tgatctcccc
    13501 gtagaactcc tcggacgtgt cgagattgtg attgtaagcg gtgagtcccg cgtccttgag
    13561 ctcgagcgcc tgctcctcgt cgagcatacc gagcgtcgcg cacgcctcca ggccgagctc
    13621 gcggacaccc cgcaccatct cgagaacctg ctggaactgc cgactgcctt tcttcgggct
    13681 tcgccaagcg gcgcccatgc aaaaacggga ggcaccggcc tctttcgcct gtcgtgcctt
    13741 gtcgagcacg tcggcgacgt ccatcagccg ttcggcctcg acaccggtat cgtacttgga
    13801 gctctgaggg cagtacgagc aatcttcttg gcacgcaccg gtcttgatcg acagaagtga
    13861 acagagctgc accgcgtccc ggggttgcgt cgcacggtgg acttcctgag ccctgaagag
    13921 cagatcggtc agaggtaagt cgtggagcgc acgggcctcc gctttggtcc agtcgtttcg
    13981 gacgtcgatc atcggtcggg ttctagcaga gagccttgaa tacgctagat tgcacgcgta
    14041 gtgtccccgg accttgaccg agggcctcga atcatcccta gcttccgtgg ctgccatcac
    14101 ctctgacgaa cacgaacgct ccgcctatct tgccgcagcc cctcctctct catggatggc
    14161 cggcgccgcg gcgctcgccg acatggtcat caatcgcctc ctggtcccgt tgggaagtga
    14221 cgcctggtct gcagccaccc tgctggagct cagccgctgg ggcggctttg cgcgcaacct
    14281 cagtgtcgtg agcgccctgg tcgcgctgtc attttgcctg gtatcgctct cgtcccctcg
    14341 aactggagtt tcgttctcgg ggcgggccgc gatctcgagc ttcggctggg tcctggtgcc
    14401 catcatcgcc ctcatgacat tcctgccccg cgccttgacc cgggtggagc tcgtcctcgt
    14461 cgtggctggg ctcaccaacg cgttaattct gcttctggtt ctggccggcg ttcagtggcg
    14521 tgcaactcgt cccgtcgcgg tgtcactcat gctcacgatg gtcgcggcgt tgagcggaat
    14581 gctgtctatg gtcgtgacgg cggtcggtgg tcgcgcgtac tgggagcaca ccgaacggct
    14641 ctcgaatgca tttcgctggt ccggcgagct tgcatacctc gccgtgccga tcgcgatcgg
    14701 gctcgccatc tcgattccct ggcgggaggc acgtggaaaa gcggcggtca tgctttccgc
    14761 tctgaccgct gcgtttgtgg caacctcgat ggtgctctcg aagcgggcgg cccgactcga
    14821 tctgccagag ctcgtttacg gcgcgatgcg cttggacttg ctaccggacg acgatttcgt
    14881 tctctatgcg atcccactcg gcgtgggctc agcggtaacc atggcagcga tactctcgag
    14941 ggacccgatt cgacgacaac tcggagcggc actgctgctg ttcctgagcg ccggctacgc
    15001 gccgcgaaca ccgagcgcac ttttgatctc ggtgctcggc gtcgcgctgc tcgcccgcgc
    15061 cggaattgca ctcgagcaac gagtgcgctg acaccctcga cgctcgcgcc ttactcgagc
    15121 ttcgacaccg cttcgccgat gcgtcgcaga ccttcgcgaa tcatctcgtt cgatgcagcg
    15181 tacgaaatac gaacgaaccc cggtgcgccg aaggcggtgc cgggaacgac ggcgcagcgc
    15241 gcttcctcga ggaggtagcc ggacacatcg aggtcggatt gcagcgtctt gccaccgccc
    15301 tgcttgccga tgagcccctg aacattcgca aacgcgtaga acgcgccctc gggcatgctg
    15361 cagctaattc ctggaatggc gttgagtccg tcgacgatga tgctccggcg ctcttcgaag
    15421 gcctttcgca tttcctccat ggggccccag ggcccgcgaa gtgcggcgac ggccgcgctc
    15481 tgtgcgatcg agctggggtt ggtcgtcgcc tgaccctgca ccttttcgca cgccttcacg
    15541 acgtactcgg gcgcgagcat ccagccgatg cgccagccgg tcatggcgaa cgtcttgctg
    15601 acaccgtcca cgatgatgat tcgatccttg agctccggag cgacctcgat gatcgatttc
    15661 tgatcaaagc cgccgtacac cagctgaccg tagatctcat cgacgatgat ccagaaattg
    15721 tgctcggcag caacatcggc aagcgcccga agctgcgctc ccgtgtacgc cgcgcccgtc
    15781 gggttcgacg gcgagcaaag aatgagggcc ttcgtcttcg gcgtgatcgc cgctcggagc
    15841 gcttcaggcg tcatgctgaa gccgttctct tcactcgtct cgacgatgac gggtttggcg
    15901 ccagcgagga gcacctgctc cgggtagctc acccaatacg gtgccgggat caagatctcg
    15961 tcgccttcat cgtagagcgc caaggctagg ttgaagagcg tatgcttcgc tcccacagac
    16021 accaggactt ccgacgggcc gtaagtaaca cctgcgcgac gctttgcgga cgcttcacag
    16081 atcgccttgc ggagctcgac ggttcctccc accgccgtgt agcgcgtggc accggagtcg
    16141 atcgctttct tggcggcttc accgatgtga gcgggcgtgc cgaaatccgg ctcgccgacg
    16201 ctgaaggaaa ggacatcgat accctgctcc ttgagctcac gagcacgctg ggcgactgcg
    16261 actgttgcgg aaggcttcac ggcgctgagc cgctgcgcta cggggattct ttcactcatg
    16321 ccgcgggctt aacaccgaag cggagtccta tcaacgctta gcgtcggaag cagctgccag
    16381 ggcgcgtaga tcgcgctcga gctcgccaat accgtcgttg ttcgcgcggt agtagccccg
    16441 aacccgggcc tctttgtcga ccagtacgaa ggagttcgag tgcatgatgt cgtcagcgcc
    16501 ggcggccctg ctgtcttcgt ccccgatcgg cacccggaag ccctcgacca ccacgtgccg
    16561 aatcgcttcc tcgcttcccg ttacaaagcg ccaattgcgt tcgtcgaggt cgttcttgac
    16621 catgtaggcc ttgatcacct cgggggtgtc gttccgggga tcgacactca ctgagacgaa
    16681 gctggcgccc gcaacgtccg aaagtcggga ctggagtccc ttcatgtggg cagtgatccg
    16741 cgggcaggtc gagcggcagt gagtgaagac gaatgccgaa acccaaaggt tcccagccat
    16801 cgattcacga gtgaaggcct gctcgttctg gtcgatcagg gaaaactcgg gaagctcccc
    16861 cagcacgggc acgtcggccg cgctcttgga gcagccctgg gccgcgagga ccaagaggcc
    16921 ccagaggccc agcagcacga ggcctgaggc cttagagcgc atcgactatc agagccgcaa
    16981 aaagagcggt gagatagatg agagaggcaa ggaagaactg cttcgcccag accttgtcgt
    17041 cctcggcttt gaagcctagg agcgccttcc agaagaagta agcgccaaga accagggccg
    17101 caccgaggta aatcgtgcca gccaaaccgt agggcacgag catgatggaa accagccaca
    17161 gcgcgcccgc atagatgacc gactgcagct tggcggatcg gattccgtga accgagggaa
    17221 gaaccttgag gcctgcccgc tcgtactctt cctgtcgaaa gatcgagatc gcaatgaagt
    17281 gcgggatttg ccaaaagaaa agcacgccga acaagacgag ccccggcacg tcgatcctgc
    17341 cggtgacggc agtccagccc atgagcggcg ggagggcgcc ggggaccgcg cctacgaaga
    17401 gcgccgccgg cgtgtactgc ttcatcggcg tgtagatcgc gacgtagctc gcaagtgcga
    17461 cgaacccgag caagccagta acgggattga ccgccaagac gagggtcggt accgcgaaca
    17521 agcccatcag aacaccgagt gtcagtgccc aggaaggctc cagccggcga tcgggaagcg
    17581 gacggttggc agtgcgtgcc atcagccggt cggtgtcacg ctcgagatag cagttgagcg
    17641 tgtttgccgc gccgacgacc atgcccgtcg tgagcagcat gacagccatg gagcggatgc
    17701 tgagggaccc cggggccatc cacattccgc ccgctgccgt cacgagaacc atcagcgtaa
    17761 tccgcggctt ggtgagcgcg atcagatcgc gcacggtcag gatcatcgtg gctgggtgag
    17821 tcgctacggt cattgggggt tccaggctgg aagcgggcgc gcactctgac gctcccgccg
    17881 aaaacggtca agctgcctcg gaggcgcagc tagggccagg ctttccctag gtcaagaacc
    17941 cggtccgaag gccgccagca gcgccagaat tcctgcgacc gccagcgcga cacccacgcc
    18001 gagcatgatc catggggttt tgggtcttgc ttcgcctcgg agcggctcga tcgctggggc
    18061 cggatcccga acgggcgccg cgggaatggg tgtgggtagg ctgatcgcct cggcctctcc
    18121 ggcctcgacc tgaagcacgt tctcgagcgc ggcggcgaac tcggccgccg acgcgtatcg
    18181 atcctccggc ttcttcgcga gggcagccat gaccacatct tcgaggccgg gtgggaaatc
    18241 cgtctccggg cgtcgttggg acaaagggat tggtgtcgcg ttcacgtgga gctgaatgaa
    18301 ctcgatcggc tgattggcat cgaacggcag cttgccagtc aagagctcgt agagaatcac
    18361 accgagcgaa tagatgtcgg atcgcgcgtc gagtgtcttt ccgtgcgcct gctcggggct
    18421 catgaactcg ggcgtaccga ataccatgcc ctgctgcgtg agcaccatcg agcccggtct
    18481 catttgcctc tcggtaactt tcgccagacc gaagtcgagc accttgggga agtcgcgaat
    18541 gcccccctgg ctggtcacga agatgttctc cggtttgagg tcacggtgaa cgatccccgc
    18601 acgatgcgct tcttcgagtg cgccgcagac ctgaatcatg atccgcatgg ctcgcttggg
    18661 cttcatcgca ccctcggctc gcacgatctg agcgagattc ctgcctacga gatgttccat
    18721 cacgaagtaa caggcgccgt catcgagctg accgtagaga aacacgcgcg cggtgttcgg
    18781 gtgcgagagc tggctcatcg cgcgcgcttc acggcgaaag cgcgacacca gatcgctccg
    18841 cgatagatat cgtgaatgaa gaatcttgat cgctacgtag cgattcatgt cgggctgctc
    18901 ggccttgtag accgccccca ttccgccctt gccgattttc tcgacgatgc ggaactgccc
    18961 agctacctct ttgttgagat acggatcgct cacgagacaa ggttagcatc cgacgtcggc
    19021 gtcggtgagg aaagtgcaag cagcgtaaga caaatccacg tcgcatcggc gatgaggaga
    19081 tgaacgagtt gcgtggcgac gggagcaaga agggcaatgt tgattaaccc cactgcgatt
    19141 tggatcagca ccagtgcgcc gagcgccagg gctaggagcc gaacccatcg gtcgggcgta
    19201 cggcgaagga agaccgacgc gagccaaatc aagtagctag aacccaggat cgcgatgacc
    19261 gggtgccaga gacggagccg caagaacaga tgagaggtcg gggagagatc ctccagcaag
    19321 ccgtcggcca ggctcgatgc gggaaacagc gtgtccccga gcgcggcaac tgcgccactg
    19381 acgccgacga gtaagacgac cgccgcagaa cccccgagca ggagccggct acgccggtcg
    19441 ccaggaaatc gggtgccctg cttttggaag gcactgaatg ccatccaagt gagcgccgac
    19501 aacaagagga aggtgttgac tagatgggcc gacatccacg cggcccgcgc agtcgatgga
    19561 ttgtcggcca ccattcgaaa gagcacgagg cctgcaccga cgagcgcctc ggtgctcatg
    19621 aagaacaagc tccagcccgc cgccgaacga gtagcatgcc cgcgttcgaa gattcgaagc
    19681 gcggcgatcc acatcagcag ggtccagagc cagcaaaggc cggaagtcac gcggtgagtc
    19741 agctcgacga tcgtcttgag cgacggcgct accggtaggg ccacgccgtt acacaatggc
    19801 cagtgcgcac cacaaccatc cccggagagc gttgcccgga cgtacgcgcc ccagagcacg
    19861 accgctactg tgtagacgag tagcgcagac gccgcgaccg caaacctatt gggtgaccgc
    19921 accgcagaac gatggagtcg aactcagccc tcgtcaacga tggtgaatcg cagcgtgtgg
    19981 ggttctccat cgcggcgata gtccacgacg agcttcgaag cggttcgcag gtcttcccag
    20041 acggcgatcg cttgctcagg acgctcgatg ggcttgccat tgacacgcgt gaccgtatcg
    20101 ccggggcgga gatcgagaga cgcgaactca ggctcgcctg ggaacaaact caagatccgg
    20161 aatccaacga aggcgccctt gtgaaatgca ggctcggttt cgacgttttg taggaacctg
    20221 ccgaggccgc cgtccagcac cgggtcgagc gcctcgcgcg caatgacacc agtggagacc
    20281 cgcgggtagg attgttcgag actgctctcg ggcttggatt cctcggccgg cgccgcacca
    20341 tccatgggat cgtgcttctc ctcccaagcg tcgttccgtt tgggcggatc ctcgacggct
    20401 ccgctttcga tgggctcctc cccgacgctg gtttcccagg tcgagtcgtc ggtcagatcc
    20461 tcgtagagct tgctcttatc gcagccaaac gtggtcgcga cgaggatggc agcgaacagt
    20521 gcagtcagat agtggctcat gcggactccc ctcgtagcgc tgtgacctgc gaccaaatcg
    20581 cggtagccac ggcgtcgaca ctttgattcg catcaatatg gtgaatcgta tcatctggga
    20641 agagctcgtc gattttcgcg tagaacttgg cgagctcggc ctgcatctcg tcgacttcgt
    20701 agagctctgg gctgcccgaa cgggtccctc gacgacgtgc agcttgcgcg ggatcgacat
    20761 cgagcaccag cgtcaagtcc gggcgtcgtg cgcgccggtt gagctcacgc acccagttgg
    20821 acacttcgac gccgctgccg ctgctcaccg tttggtaggc aaccgaggaa tggtcgtagc
    20881 gatcgcaaat gactgtgacg ccgtcggtga ggttcgggat gatctccgct tggagatgat
    20941 cgaggcggtc ggaagcgaac agcgtcgcca tcgttcccca gttgggcggc cggaccccgt
    21001 gccgaccggg caccacgacc ctccctgtca ggatctgacg aatcaaaaca ccgatgggtc
    21061 cgtcgctcgg ttcgtgcgtc gtgtgcacgg gaagcccctt ctcgctcagc cgatcggcaa
    21121 ggatcgcgct ttgggtcgtc gtgcccgatc cgtcgatgcc ttcgagcacg atgaaatggc
    21181 cttcgatcac gagccctcct tcgcgccgcc gaacttgaaa gtcgctccga actggccgta
    21241 gaccttggag tcggggtgcc cgccgccgaa gatgtcgcca agaacccgtc gccgatcacc
    21301 agcaacgatg ccctcgaatt ggagccacac gttccacttc tgcgtcacga cgatttccac
    21361 tgagccgcca aggcgagcac gcgcgagatt ctgccgtgag cttgtcacat aaacattgct
    21421 attggtacct tcaaagtccc atcggtcact gttgaaatcc cccgcgatcc agacggagaa
    21481 cgcacccctc ggcggaaagt ggaggctcaa gatcgcgtcg agactgatga agaagttgtt
    21541 ggtcgggtgg cttccgccgt ctacgtcgcg gtgcgacgga cccacgccac cgccgacccg
    21601 aagatctccg ccgacggaaa agtagcgaga aacacgccag ccggccttcg tgctgcccca
    21661 gaaatcggtg agacgcccaa acgtggccac gtagatgccc acgtcgagaa acggcttgag
    21721 cccggcgcgc agccccaccg acgccagcca tggccagccc gtggagaagt ccaaggttgc
    21781 cacgctcgca gggtttggag cgcccgcatg agtcacggtg tttcgaagcg gatcactgcg
    21841 ttcggattct ttgtcattct tgctctggtt cgccggcgct gcggcagagc tcaggatcac
    21901 ctggatcaac cggatgcgtg gcgacttctg caaccgaatc tgctccacgt ggtttccgta
    21961 tcctgcagcc ctcacctcca gctgatgggt gccggcggcg agatcgccct cccaggggac
    22021 ggggcctaga agctcgccat ccacgtagag ttgtgcttgt gaaacgtttg cttcgacgcg
    22081 aacaggcaca ccgaggggat tgagctccgc gtacacatcg cagtttcgag tgggtccgac
    22141 ggaacacgtg cggcgcacct cacgaaaacc tggggcccgc acgacgatcg agtgcgttcc
    22201 gaactccgct ggctcgatgg caatcggcgg gtttccgtaa tcctcgccat cgattgtgac
    22261 cgtggcctcg ggaacgctgg cgcgaaccag gattcgcgcc acatccgtgc tcgccgtggt
    22321 gaacctgagg gagacgactc gttcccgccc cgcaatcacg gtgacggcct gatcgaccgc
    22381 ctggtagccg cttgcgcgcg ccatcacgac gtgctcaccg ggcgtcaatc cgcctttggc
    22441 ggcaggggca acaccgatgt cgattccatc gagactgatg atcgcaccag gcacgttcgt
    22501 gatcacgcgc agacttccca ggtcgggtgc tatgcgaatc gtcgcctcca cggtcgtccg
    22561 ctcgctcgcc cggatcgtca ctttctcgaa gaacggttga tagccctcgc ccgggctgcg
    22621 gatctcgacc aggtgctcac cctcgctgag gccatccacg accagagggg ttgccccgcg
    22681 ctgctgtccg tcgatgaaaa ccggaatccc cgtgacgtcg gcagtgacca ggagcgagcc
    22741 ggtcttggcc gcctgacgtt gaagcacgac cggaatggtg agaacttggc cgccgatcaa
    22801 gtccacccac ttcgagaaag tcacgaagcc gcgtttgcca acttgcacca ggtgtcggcc
    22861 agggctgagc gtcgtgcgga ccggaacggt gccagatggc tcgccatcga tgcgaatcgc
    22921 cgcgccggaa gtgg
//
LOCUS       DASRZF010000347         3662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_608174, whole genome shotgun sequence.
ACCESSION   DASRZF010000347 DASRZF010000000
VERSION     DASRZF010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608174"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="VFG22_15480"
     CDS             <1..784
                     /locus_tag="VFG22_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET6417698.1"
                     /translation="VQPLANGALVCALDTDFDGCMEIVQKLTTEQGIYLANSMNSLRL
                     EGQKTVAIEIVQQFDWKVPDWVIIPGGNLGNVAALAAGFDMMFDLGLTSKRPRICLAQ
                     AEKANPLYRAYKQGWDKFEPMTAGDTEASAIRIGNPVSVRRAIRALQAFDGVVEQASE
                     SELAEASAAADRAGSYTCPHTGVALAALEKLRAKGLIEPGQRVVVISTANGLKFTQFK
                     IRYHENEIPGVESRHPNRPVHLPSEFEAVKSAVFRELEKRAV"
     gene            complement(791..1072)
                     /locus_tag="VFG22_15485"
     CDS             complement(791..1072)
                     /locus_tag="VFG22_15485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417699.1"
                     /translation="MKLRLESGEDAEARAFDGSHFTLWSPRAFAPGSPVRFLAWFEEG
                     ERPLEGRALGSKRVDEHQFEVRVRFVNLRRDDRQRLLEQLESQRFDESD"
     gene            complement(1077..1529)
                     /gene="smpB"
                     /locus_tag="VFG22_15490"
     CDS             complement(1077..1529)
                     /gene="smpB"
                     /locus_tag="VFG22_15490"
                     /inference="COORDINATES: protein motif:HMM:NF003843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HET6417700.1"
                     /translation="MELLVCRNPKAAQRYDIDERLEAGMVLYGTEVKSLRARRANLEG
                     AYCRIDNDELFLFGMHIAAYEQGGHAGHETQRRRKLLVHKREIENLRGRLTMRGYALV
                     PLQVYFKNGVAKVQLGLGRGKKKGDERESIRRQEDLKEARAAMERQKR"
     gene            complement(1546..2322)
                     /locus_tag="VFG22_15495"
     CDS             complement(1546..2322)
                     /locus_tag="VFG22_15495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417701.1"
                     /translation="MRRLIPCCAFLLSCVQPEPVQDPLDYLRVGVDPAPEADVIMEDL
                     KRHGFRIGRRVEQTDFVAFDAFRGPDSTVRVVTSRGPSFAIQVPDARWPERLRVELDD
                     HPPPDFDRDGRRDVVVAIHEHERTCLAWAEVDTLGFVEEVFRANADWGQSPCVIEIDP
                     AWPRLLLEVSVPDSPVPNAKVRLPIKASARSWVLDESPFASARWERERQQREDALQSA
                     ESQDDSSAAERLRVELGWLEQLRKAEDPVLEPASDVEEAR"
     gene            complement(2332..2955)
                     /locus_tag="VFG22_15500"
     CDS             complement(2332..2955)
                     /locus_tag="VFG22_15500"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HET6417702.1"
                     /translation="MCTIVRGFLALLAPLRCAACDEPVDEPVAFCGACSASAQRATLP
                     GAVFEYGGPVADAIQRFKYGGRSELAAALGLLMTADAIRWGGQVDAVVPVPLHWRRRR
                     TRGYDQAALLAKPVAKALAVPPLLRALRRVRNTPSQVDLPFRERQRNMRRAFAPSRIR
                     GARRVLLIDDVRTTGATIRAASEALKAGGAVEVHSLVLAARVLTEAT"
     gene            complement(3437..>3662)
                     /locus_tag="VFG22_15505"
     CDS             complement(3437..>3662)
                     /locus_tag="VFG22_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HET6417703.1"
                     /translation="HDLAQWLGRAENESVQTVVFLIGGSYGLPEALSKQADLRLSLSA
                     MTLPHRLARLFLAEQIYRGFSILRGEPYDH"
BASE COUNT          680 a         1175 c         1177 g          630 t
ORIGIN      
        1 ggtgcagccc ttggcgaacg gtgccctcgt gtgtgcgctc gataccgact tcgacggttg
       61 catggagatc gtccagaagc tgaccaccga gcaagggatc tatctcgcga actccatgaa
      121 ttcgctccgc ctcgaaggac agaagacggt cgccatcgag atcgtgcagc agttcgattg
      181 gaaggttccc gattgggtga tcatccccgg cgggaatctg ggcaacgtcg cggcgcttgc
      241 ggcgggcttc gacatgatgt tcgacctcgg actcacctcc aagcgcccgc ggatttgcct
      301 cgcgcaggcg gaaaaggcga atccgctcta ccgagcgtac aagcagggct gggacaagtt
      361 cgagccgatg accgcgggcg acaccgaagc cagcgcgatt cggattggca acccggtgag
      421 tgtgcgccgt gcgattcgcg cgcttcaggc cttcgacggc gtggtcgagc aggcaagtga
      481 gtccgagctc gccgaagcgt ccgctgcggc cgatcgagcc gggagctata cctgcccgca
      541 caccggcgta gcgctcgcag ccctcgagaa gctccgagcc aagggcctga tcgagcccgg
      601 ccaacgcgtc gtcgtcatct cgacggcaaa tggactgaag ttcactcagt tcaaaatccg
      661 ctaccatgaa aacgaaattc cgggcgtcga aagcaggcac ccgaaccgac ctgtgcacct
      721 tccgagcgag ttcgaagcgg tcaagtcagc cgtctttcgc gagctcgaaa agcgcgccgt
      781 ctagcagtat tcagtcgctt tcatcgaagc gctggctctc gagctgttcc agtagccgtt
      841 ggcgatcgtc acgccgcagg ttgacgaagc gcacgcgcac ctcgaactgg tgctcgtcga
      901 cccgtttgga accaagcgcg cgaccctcga gcgggcgctc gccttcctcg aaccatgcca
      961 agaagcgaac cggagacccc ggcgcgaagg cacgcgggct ccagagcgtg aagtggcttc
     1021 cgtcgaaggc gcgagcctcg gcatcttcgc cgctctccaa gcgaagcttc acagggtcat
     1081 ctcttctgac gctccattgc ggcgcgcgcc tctttgaggt cttcctgacg gcgaatcgac
     1141 tcgcgttcat cgcccttttt cttgccgcgt ccaaggccta gttgaacttt ggccacgccg
     1201 tttttgaaat agacttgaag gggaaccaat gcgtagccac gcatcgtgag gcgtcctcga
     1261 aggttttcga tttcgcgctt gtgcaccagg agcttgcggc gacgctgcgt ctcgtgcccg
     1321 gcgtggccgc cctgctcata cgcggcaatg tgcatgccga agagaaagag ctcgtcgttg
     1381 tctattcggc aatacgcgcc ttccaggttg gcgcggcgcg cccgcaagct tttcacctcg
     1441 gtgccgtaga gcaccatccc tgcttcgagc cgttcgtcga tgtcatagcg ctgggctgcc
     1501 ttcggattgc ggcataccag gagctccatc ccaggaactc ctttgtcaac gcgcttcttc
     1561 gacatcgctt gcgggctcta gcacggggtc ttcggctttg cgaagttgct cgagccagcc
     1621 gagctcgacg cgcaagcgct cggcggcgct ggagtcgtcc tgcgactccg ccgattggag
     1681 cgcatcttcg cgctgctggc gctcacgctc ccaacgcgcg ctggcaaagg gagactcgtc
     1741 caagacccag gatcgcgccg atgccttgat cggcagccga accttggcat tcggaaccgg
     1801 cgagtctggc acggagactt ccagcagcaa gcgcggccac gcgggatcga tttcgatgac
     1861 gcaaggggat tgcccccaat cggcgtttgc gcgaaacact tcttcgacga accctagtgt
     1921 gtcgacctct gcccaggcga ggcacgttcg ctcgtgctcg tggattgcga ccaccacgtc
     1981 tcgcctgcca tcacgatcga agtcgggtgg aggatgatcg tctagctcga cccggagcct
     2041 ctcgggccaa cgcgcgtcgg gaacctgtat cgcaaacgac ggtcccctcg acgtcaccac
     2101 ccgaaccgtc gaatccgggc cgcggaatgc gtcaaaggcc acgaaatcgg tttgttcgac
     2161 acgccgtccg attcggaatc cgtggcgctt cagatcttcc atgatgacat ccgcttcagg
     2221 ggcgggatcg acaccgacgc ggaggtagtc gagcggatcc tgaaccggtt cgggttgcac
     2281 gcaggacagc aaaaacgcgc agcagggaat caggcgacgc acgccgatac tctacgtggc
     2341 ttcggtgaga acgcgagccg ccagcacgag gctatggact tcgaccgcac cacctgcctt
     2401 gagggcctct gacgcggcgc gaattgtcgc gccggtggtc cgcacgtcgt cgatcagtag
     2461 aacgcgcctg gcgccccgaa tgcgggatgg cgcaaacgcg cggcgcatat ttcgctggcg
     2521 ttcgcgaaat ggcagatcga cttggcttgg ggtgtttcga acgcggcgca gcgcccgcag
     2581 caacggcggc accgcgagcg cctttgccac gggcttggcg agcagcgccg cttgatcgta
     2641 gccacgggtc cggcgcctgc gccaatgaag aggaaccggc acgacggcat caacctgccc
     2701 tccccatcgg attgcgtccg ccgtcatgag caagccgagc gccgccgcaa gctcgctccg
     2761 ccctccgtat ttgaagcgct gaatggcgtc ggcgacggga ccgccgtact cgaacacggc
     2821 gcccggcaga gtggcgcgct gggcggacgc ggagcacgcg ccgcagaacg cgaccggctc
     2881 gtcgacaggc tcgtcacaag cggcgcaacg cagaggagct agcaaggcga ggaatccgcg
     2941 gacgatggtg cacatgaccg gcgtatcgga cggattccac gaacagttgc gctggtatcg
     3001 acatcggcgc caacactggc acgaacaccg atgtcggcgg tacggcgatc ggagagcggc
     3061 tcgcgacgca cgtgctcgcg attgggaccc gcgcggccgg ctctccaagg ctccctacgt
     3121 ctcgcctccc gcccaatcgc tgtgccgcgc atcccgagcc gcccccctaa gtagataccg
     3181 acgcatgaat gccgacactg gcactggcac tggcactggg agtctatgga ggggcccggg
     3241 ggaacgcgat gcatacgtgg acgcacggag ggggacggct cgggatgggc agcgcagcga
     3301 ttgggtcgag cctagccgta ggtcgtctta gggaattggg ggcgagtggg tcccaatcgc
     3361 gagcacgccc gtcccgagcc gtcccccggt ggctcagccg cgcaacacaa ttgctggcgc
     3421 tggcactgac actggctcag tggtcgtacg gttcgccgcg caggatcgag aagccgcggt
     3481 agatttgctc ggccaggaac aagcgggcga ggcgatgggg caaggtcatc gcggagagcg
     3541 ataggcgcaa gtcggcttgc ttggagagcg cctcgggcag accgtaggag ccgccgatca
     3601 agaagacgac cgtctggacc gattcgttct ccgcacgtcc gagccactgc gcgaggtcgt
     3661 ga
//
LOCUS       DASRZF010000348         7552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2674525, whole genome shotgun sequence.
ACCESSION   DASRZF010000348 DASRZF010000000
VERSION     DASRZF010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 7552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7552
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2674525"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /gene="pip"
                     /locus_tag="VFG22_15510"
     CDS             <1..786
                     /gene="pip"
                     /locus_tag="VFG22_15510"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HET6417704.1"
                     /translation="SKYRIILFDQRGCGRSTPHAELQNNTTWHLVDDIERIRVHLGIE
                     RWQLFGGSWGSTLSLAYAQANPERVTELILRGIFLLRRREIDWYYQEGASRIFPEAWQ
                     KYLEPIPEEERGDMLSAYYRRLTSSDREERIGAARAWSMWEGSTSRLIPDPVLIERTG
                     DITFAEAFARIECHYFTNAGFLEEDDRLLRQVGIIRHIPAIIVQGRYDIVCPAESAYQ
                     LHQAWPESVLRIAARSGHSALEPEISSELVAATNELSARTSGT"
     gene            complement(761..2434)
                     /gene="ettA"
                     /locus_tag="VFG22_15515"
     CDS             complement(761..2434)
                     /gene="ettA"
                     /locus_tag="VFG22_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HET6417705.1"
                     /translation="MAEFIFTMKGVTKVHPPDKKIIEDLYLSFLPNAKIGVIGVNGTG
                     KSSLLRIMAGVDDSFTGEAWIRPGAKVGYLSQEPDLGDAKTVLEAVEVGVAPVRAILD
                     EFNELSMKLGEPMSDEEMAKLLDRQGALQDKIDAVDGWNLESTLEIAMDALRLPPADA
                     DPKLLSGGEKRRVALCRLLLEKPELLLLDEPTNHLDAESVAWLQGYLQKYPGCVILVT
                     HDRYFLDEIVEWILELDRGEAHPFHGNYSQWLEAKDARLAQEEKKDSSRKRKIARELE
                     WVRSSPKARQAKSKARITAFESLVADAAKARGDANEIRIPPGPRLGTQVFELKGVSKA
                     YGDKLLFEDLSFIVPRGAIVGIVGPNGAGKTTLFRMLVGEETPDSGTIVKGDTVALSY
                     VDQSRDSLDPSNTVFKEITGGADEIDVGGGTVKSRAYVGWFNFRGSDQQKPVKNLSGG
                     ERNRVHLAKLLKSGGNVLLLDEPTNDIDVETLRSLEEAILSFPGCALIISHDRWYLDR
                     ICTHILAFEGDSRVVWHEGSYADYAADRKARLGTDADVPHRIKYRKFSR"
     gene            complement(2438..4054)
                     /locus_tag="VFG22_15520"
     CDS             complement(2438..4054)
                     /locus_tag="VFG22_15520"
                     /inference="COORDINATES: protein motif:HMM:NF021597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HET6417706.1"
                     /translation="MKQTIAVLGLCVLTSWPTTSRAFCGFYVSGADASLYNNATMVVM
                     MREGTRTVLSMQNNYQGPPEDFAMIVPVPVVLQEDNVKTLPPEIFSRVDKLAAPRLVE
                     YWEQDPCRQMVVYEMAAGMPQAARRKATMNDEAESDLGVTIEAQFKVAEYDIVILSAK
                     DSGGLDKWLRKNEYNIPKGANEVLRPYVEQNTKFFVAKVDSKKVKFRDGKAVLSPLRF
                     HYDAPTFTLPVRLGLLNSAGKQDLIVHILGKAQRYEVANYKNASIPTNIRVEHEVRST
                     FGAFYDALFERTIEKNPGAVVTEYAWDATTCDPCPEPPLRQDELATLGADVIVPVEED
                     PYSDIPGVVPQRKVRPSWQPSGFVLTRLHYRYDKDGLDEDLVFRTAGPIVGGRGMPDQ
                     TGELGERRAQASSVNNFQGRYVMLNPWEGEISCENPQRGIWGGPPDGPTPRPFAAGNT
                     ALEGTSTKSLTSQLPALIQTDIPELDLEAGTVDLSPKGQPDSKPEQSSPTGDAGASEG
                     CSVGAAKSASLGWIALSIVAMWLGRRRTAR"
     gene            complement(4197..5159)
                     /locus_tag="VFG22_15525"
     CDS             complement(4197..5159)
                     /locus_tag="VFG22_15525"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET6417707.1"
                     /translation="MKNFWTLLSCVALAAALTACKSGASAPEEGDNAEATETEAAADE
                     KPAGASEEVAQKPVAEGAAPDAIPAPPDVAAPPETAERSASGLAWTVLQAGTGDKHPS
                     AHDIVTVNYTGWTTDGRMFDSSTKRGKPAEFPLNRVIKGWTEGVQLMVPGEKRRFWIP
                     GDLAYGEALPNDPHAAMGPPKGTLVFDVELLSFKEAPKPPDTPKDVAAIPKNAKKTKS
                     GLGSRVLKKGEGKEHPSATSVVTVNYSGWTTDGQMFDSSVMRGTPATFGLNQVIPGWT
                     EGLQLMVVGETRRLWIPEDLAYGGRPGRPQGMLVFDVELLEIKN"
     gene            complement(5192..5683)
                     /locus_tag="VFG22_15530"
     CDS             complement(5192..5683)
                     /locus_tag="VFG22_15530"
                     /inference="COORDINATES: protein motif:HMM:NF016102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HET6417708.1"
                     /translation="MGRLFLLFTVVPLIELYLLVAVGRILGPIPTIGLVLLTGAVGAW
                     FARLEGARVIRRWQEAIARQELPKDGVIDGFLIFIGGVMLITPGILTDIAGLSMVMPP
                     TRRVIAGFVRAWFERQIASGNVQVYASGYNVGRSRDQDVIDVESEVVEVVRKPDDQNR
                     LEK"
     gene            5811..6809
                     /locus_tag="VFG22_15535"
     CDS             5811..6809
                     /locus_tag="VFG22_15535"
                     /inference="COORDINATES: protein motif:HMM:NF015951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HET6417709.1"
                     /translation="MNTAADGSLGPKWGLRCLLGGALMGLANLVPGISGGTMLLAAGI
                     YPRFIESLADLSALRFRKSSIMVLFLVTGAAAVAILLGAGTVKDAVVEHRWAMYSLFI
                     GLTLGGVPVLWRMARPAANVFWAGMFAGLLVMAALAWTQVGGASSGESREGMVIMFVA
                     GVVGASAMILPDVSGGYLLLVLGVYVPVLSAIDSLKEALRANDLGSASDPILGVVLPV
                     GIGVAVGVLLISNLIKTVLERFEQPTLGVLMGLLVGAVFGLWPFQEGVAPKVGEIFKG
                     QALTAERLAALTPDKYPTAIYTPSFTDVMMAIGLIGAGYMITSLVAQIGGTAPKGR"
     gene            complement(6821..>7552)
                     /locus_tag="VFG22_15540"
     CDS             complement(6821..>7552)
                     /locus_tag="VFG22_15540"
                     /inference="COORDINATES: protein motif:HMM:NF027666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET6417710.1"
                     /translation="DKEHIEALHDGEISYHDLYFGKFLVKLRELGLDENTILVVTSDH
                     GEEFQEHGSWGHGHSVYQELLGVPLIFRWPGVIPAKTRIAPVVSTMDIGPTVLEATGV
                     AIPDVFEGHSLLGFMLGDWPTGPYVAFSDFLDHRRVIRGGDWKMIIRGNLSQVMFDLG
                     NDYWEKKELDGSAQPIAQRYLRTLHGQFLGADNRGRWLAASSVAAGKNKPALTEEKQE
                     MTPELCRQLVALGYISAECDALLDN"
BASE COUNT         1361 a         2352 c         2308 g         1531 t
ORIGIN      
        1 tcgaagtaca ggatcatcct cttcgatcag cgtggttgcg gccgaagtac gccccacgcg
       61 gagttgcaaa acaacaccac ctggcaccta gtcgacgaca tcgagcgcat tcgtgtgcat
      121 ctcggtatag aacgctggca actgttcggg ggctcctggg gcagcacgct ttccttggcc
      181 tacgcgcaag cgaatcccga acgcgtcact gaattgatcc tgcgcggcat cttcctgctc
      241 aggcgccgcg agatcgactg gtactaccaa gaaggcgcga gccgaatctt cccggaagcc
      301 tggcagaagt acctggagcc gatcccggaa gaagagcggg gagacatgct gagcgcgtac
      361 taccgccgac tcacgagctc ggatcgggaa gagcgcatcg gcgccgcgcg cgcatggagc
      421 atgtgggagg gcagtacgag ccggctgatt ccagaccccg tgctgatcga acgcaccgga
      481 gacatcacat ttgcggaagc gttcgcccga atcgaatgcc actacttcac aaatgccggc
      541 ttcttagagg aagacgatcg actgctgaga caggtcggca tcatccgcca cattccggcg
      601 atcatcgtgc agggccgcta cgacatcgtt tgccctgccg agagcgccta tcaacttcac
      661 caagcctggc cggagagcgt tttgcggatc gccgctcgtt caggtcactc ggctctcgaa
      721 cccgagatca gctccgagct cgtggccgca accaacgagc tcagcgcgag aacttccggt
      781 acttgattcg gtgaggaacg tcggcgtcgg tgcctagccg ggcctttcgg tcggcagcgt
      841 agtcggcgta agaaccctcg tgccagacca cccggctgtc gccttcgaag gcgaggatat
      901 gcgtgcagat tcggtcgagg taccagcgat catggctgat gatcagcgcg cagcccggaa
      961 agctgagaat cgcctcttcg agcgaacgca acgtctcgac gtcgatgtcg ttcgtgggct
     1021 cgtcgagaag cagcacgtta ccaccgctct tgaggagctt ggcgaggtga acgcggttgc
     1081 gttcgccgcc ggataggttc ttgacgggct tctgctgatc gctcccgcgg aaattgaacc
     1141 agcccacgta ggcgcgtgac ttcacggtgc cgccgcccac gtcgatctcg tcggcgcctc
     1201 cggtaatctc cttgaagacc gtattgctgg ggtcgagcga atcgcggctc tgatcgacat
     1261 acgaaagcgc gacggtgtcg cccttcacga tcgttccaga gtccggggtc tcttcgccaa
     1321 ccagcattcg aaacaaagtc gtctttccgg ctccattcgg tccgacgatg ccgacgatcg
     1381 caccgcgcgg cacgatgaag ctcaagtctt caaaaagaag cttgtcgccg taagccttgc
     1441 tcaccccttt gagctcgaac acctgcgttc cgagtcgtgg cccaggaggg atgcgaatct
     1501 cgttggcgtc accgcgagcc ttcgccgcgt ctgcgacgag gctctcgaat gccgtgatgc
     1561 gcgccttgct cttggcttgc ctggccttcg gcgacgaacg cacccactcg agctcccgcg
     1621 cgatcttgcg ctttcgagag ctgtctttct tctcttcttg ggcgagccgt gcgtccttgg
     1681 cttcgagcca ctgcgagtaa ttgccgtgga aagggtgcgc ctcaccgcgg tctagctcga
     1741 ggatccactc cacgatctcg tccaagaagt agcgatcgtg ggtgaccaaa atcacgcagc
     1801 ccggatactt ctgaaggtac ccctgcagcc aggcgaccga ctcggcatcg agatggttgg
     1861 tgggctcgtc gagcaagagg agctcgggct tctccaacaa caggcggcaa agcgcgaccc
     1921 tgcgtttctc gccgccggaa agcaacttcg gatctgcgtc ggccgggggc agtcgaagcg
     1981 cgtccatggc gatctccaac gtgctctcta gattccagcc atccactgcg tcgatcttgt
     2041 cctggagcgc tccctgtcga tcgagcagct tggccatctc ctcgtcgctc atcggttcgc
     2101 cgagcttcat gctgagctcg ttgaactcgt cgaggatcgc gcggacgggc gctacgccga
     2161 cttcgacggc ctcgagcacg gtcttggcat ccccgagatc cggctcctgc gaaaggtaac
     2221 ccaccttcgc gcccggacgg atccacgcct ctccggtgaa gctgtcatcg acccctgcca
     2281 tgatgcgcag gagcgacgac ttgccggtgc cattcacacc gatgacccct atcttcgcgt
     2341 tcggaaggaa cgaaaggtag aggtcttcga tgatcttctt gtccggggga tgaactttcg
     2401 taaccccctt catcgtgaag atgaattctg ccatgagcta tcgagcggtg cgtctcctgc
     2461 cgagccacat agcaacgatg ctcagcgcaa tccaaccgag cgaggcgctc ttcgccgcgc
     2521 cgacgctgca accctcggag gcgcccgcat ccccagtggg actgctctgc tcgggcttgg
     2581 aatcaggctg gcccttgggc gagaggtcga cggtgccagc ctcgaggtcg agctcgggga
     2641 tgtcggtttg gatcaaggcg ggcagctggc tggtcaggct tttcgttgaa gtgccttcga
     2701 gtgcggtgtt cccggcggca aagggccgag gcgtcggtcc gtccggcggg ccaccccaga
     2761 tgcctcgctg gggattctcg cagctgatct cgccctccca aggattcagc atcacgtatc
     2821 gtccctggaa gttgttcacg ctcgaggcct gcgcacgccg ttcgccgagc tctcccgttt
     2881 gatcgggcat gccacggccg cccacgatcg ggcctgcggt gcgaaacacc aggtcttcgt
     2941 cgagtccgtc cttgtcgtag cgatagtgca agcgcgtcag cacgaagccg gagggctgcc
     3001 aagatggcct aaccttgcgc tgtggaacca ctccagggat gtcggagtac gggtcctcct
     3061 cgacgggcac gatcacgtcg gcgccaagcg tcgcgagctc atcctgcctc agcgggggct
     3121 ccggacacgg atcgcaggtc gtcgcatccc aggcgtactc ggtgacgacc gctcccgggt
     3181 tcttctcgat ggtgcgctcg aagagtgcgt catagaacgc cccgaaggta ctgcgaacct
     3241 cgtgctcgac gcggatgttc gtcgggatcg acgcgttctt gtagttggcg acctcgtagc
     3301 gttgagcctt gccgaggatg tgaacgatca agtcctgctt gcccgcgctg ttgagcaagc
     3361 cgagccgcac gggcaaggtg aaggtgggcg catcgtagtg gaatcgcagg ggtgagagta
     3421 ctgccttgcc atcgcgaaac ttcaccttct tcgaatccac cttcgcgacg aagaacttcg
     3481 tgttctgctc cacgtaggga cgaagcactt cgttggcgcc cttggggatg ttgtactcgt
     3541 tcttgcgcaa ccacttgtcg agtccgcccg aatccttcgc gctcaagatg acgatgtcgt
     3601 actcggcaac tttgaactgt gcctcgatcg tgacacccaa gtcggactcc gcttcgtcgt
     3661 tcatcgtggc ttttcttcgc gccgcttggg gcatgccagc cgccatttcg tagacgacca
     3721 tctgccggca aggatcctgc tcccagtact cgaccaggcg cggcgccgcg agcttgtcca
     3781 ctcgcgaaaa gatctccggc ggcagcgtct tcacgttgtc ttcttgcaag accaccggta
     3841 ccggcacgat catcgcgaag tcttctggtg gaccttggta gttgttctgc atcgagagca
     3901 cggttcgtgt gccctcgcgc atcatcacga ccatcgtggc gttgttgtag agcgaggcgt
     3961 cggccccact cacgtagaac ccacaaaacg cacgtgacgt agtcggccag gacgtcagca
     4021 cgcagagacc cagaacagcg attgtttgct tcattgattg ctcctcggtt gtcctggtag
     4081 acgcccgagc tcgcgatttg gatctaactc gagaaaatcg cttgattacg gcatgatggc
     4141 aatcagctcg atgtcgaagg cgacggtgcc catcggccgg cgcacagcct tatcgtctag
     4201 tttttgatct cgagaagctc gacgtcgaac accagcatgc cttgcgggcg tccggggcga
     4261 ccaccgtagg cgagatcttc cggaatccaa agccggcgcg tctcaccgac gaccatgagt
     4321 tgcaagccct cggtccagcc gggaatgact tggttcaagc cgaaggtggc tggggtgcca
     4381 cgcatcacgg agctgtcgaa catctggccg tcggtggtcc aacccgagta gttgacggtt
     4441 acgacgctgg tcgcgctcgg gtgctctttg ccttcgccct tcttcaagac acgcgagccg
     4501 aggcccgact tggtcttttt cgcgttcttg ggaatggcgg ccacgtcctt gggtgtgtcg
     4561 ggcggcttgg gtgcttcttt gaagctcaag agctcgacat caaacacgag cgtgcccttc
     4621 gggggaccca tcgcagcgtg cggatcgttc gggagcgctt cgccatatgc gagatctcca
     4681 ggaatccaga atcggcgttt ctccccaggg accatcaatt gaacgccctc cgtccaacct
     4741 ttgatgacgc ggttgagcgg aaactcggcg ggcttgcctc tcttcgtgga gctatcgaac
     4801 attcgcccgt ccgtcgtcca acccgtgtag ttgacggtca cgatgtcgtg cgcgcttgga
     4861 tgcttgtcac cggtgccagc ttggagaacg gtccaagcga gacccgaggc gctgcgctcg
     4921 gcggtctcgg gtggcgccgc cacgtcgggg ggggcgggaa tggcatccgg tgcagcacct
     4981 tcggcgacgg gtttttgtgc gacctcttcg ctcgcgccgg ctggtttctc gtcggcggcg
     5041 gcttcggttt cggttgcctc ggcgttgtca ccctcttcgg gggcggatgc gccgctcttg
     5101 caggcagtca acgcggcggc gagcgccacg cagctgagaa gggtccagaa atttttcatg
     5161 tgggcttggt agccgactga acgcgcgctg tctacttctc gagtcggttc tggtcatcgg
     5221 gtttccgcac gacctcgaca acctcgcttt cgacgtcgat cacgtcttgg tcccgagagc
     5281 ggcccacatt gtagcccgag gcgtacactt gtacgtttcc gctcgcgatc tgtcgctcga
     5341 accatgcgcg gacgaacccg gcgatcacac gacgggtcgg aggcatgacc atcgaaagac
     5401 cggcaatgtc ggtcaggatt ccaggcgtga tcagcatcac gccgccgatg aaaatcagga
     5461 acccatcgat gacgccatcc ttcggaagct cttggcgcgc gatggcttcc tgccaacggc
     5521 gaatcacccg cgctccctcg agtcgcgcga accaggcgcc caccgcgccg gtgagaagca
     5581 ccaaaccaat cgtcgggatg ggtccgagga tacggccgac cgcgaccaga aggtacaact
     5641 cgatgagggg aacgacggtg aaaagcagga aaaggcggcc cacccaatga acctaggtcc
     5701 gatccccgga ctgcgctacc cagcccctag aaatgcagcg accgtcgttt gcctgcgagt
     5761 agcgcgcgct ttgcttggcg tgttcccgta tctgcggtac gcccggccgc atgaacacag
     5821 cggcggacgg gtccctgggc ccgaaatggg gcctgcgttg cttactcggg ggtgcattga
     5881 tgggactcgc caatctcgtc ccgggcatca gcggcggcac gatgttactc gctgccggca
     5941 tttatccgcg cttcatcgag tcactcgctg acctcagcgc acttcggttt cgcaagagct
     6001 ccatcatggt cttgttcctc gttaccggag cggctgcggt agcgattctg ctcggggcgg
     6061 gcacggtgaa agatgccgtc gtcgagcacc gatgggcgat gtacagcctc ttcatcggtt
     6121 tgacgctcgg cggagtgcca gtgctttggc ggatggcgcg cccagccgcg aacgtgttct
     6181 gggctggcat gttcgctggg ctcctcgtaa tggcggccct ggcctggact caggtgggcg
     6241 gcgcaagctc gggtgaaagc cgtgagggca tggtgatcat gttcgtcgcc ggtgtggtgg
     6301 gcgccagcgc gatgattctc ccagatgtga gcggtgggta tctgctgctg gtgttgggcg
     6361 tttacgtccc cgtgctgagc gcgatcgact cgctcaaaga agcgctccgc gccaatgacc
     6421 tcgggagcgc ctcggacccg atcctcggcg tcgtgcttcc ggtaggcatc ggcgtagcgg
     6481 tcggcgtcct gctgatcagc aacctgatca agacggtcct cgagcgattc gagcagccaa
     6541 cgctcggcgt gctcatgggc ctgttggtcg gcgcggtctt tggcctctgg ccttttcagg
     6601 agggcgtcgc gccaaaagtc ggcgagatat tcaaagggca ggctttgacc gccgaacggc
     6661 tcgcggcact gacaccggac aaatatccaa ctgcaatcta cacgccgtca ttcacagacg
     6721 tgatgatggc gatcggtctg atcggtgcgg gatacatgat cacctcactg gtcgcccaga
     6781 tcggcggcac agcgccgaaa ggccgctaga gctcggcgtc tcaattatcg agaagcgcgt
     6841 cgcactcagc gctgatgtac cccagcgcca cgagctgtcg gcagagctcc ggggtcatct
     6901 cctgcttctc ctccgtaagc gcgggcttgt tcttccccgc ggcgacggag ctcgctgcca
     6961 gccatcgacc tcggttgtct gcgccgagaa actgcccatg aagggtgcga agatagcgct
     7021 gcgcaattgg ctgagcgctg ccatcgagct ctttcttctc ccaatagtcg ttcccgaggt
     7081 cgaacatgac ctgcgagaga ttccctcgaa tgatcatctt ccaatcaccg cctcgaatca
     7141 cgcgccggtg gtccaggaaa tcgctgaagg cgacataagg acccgtcggc caatcgccga
     7201 gcatgaagcc gagcaagctg tgcccctcga acacatccgg aatcgctaca cccgtggcct
     7261 cgagcaccgt cgggccgatg tccatcgtgc tgacgaccgg agcaattcgg gtcttggctg
     7321 gaatgacccc cggccaccga aagatcagcg gtacaccgag aagctcttga tacacggagt
     7381 gcccgtgacc ccaagagccg tgctcctgga actcctcgcc gtggtccgaa gtcaccacga
     7441 ggatggtgtt ctcatcgagg ccgagctcac gaagcttgac gaggaatttt ccgaaataca
     7501 ggtcgtggta gctgatctca ccatcgtgca gcgcctcgat gtgctctttg tc
//
LOCUS       DASRZF010000349         6937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4394981, whole genome shotgun sequence.
ACCESSION   DASRZF010000349 DASRZF010000000
VERSION     DASRZF010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 6937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6937
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4394981"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /locus_tag="VFG22_15545"
     CDS             <1..805
                     /locus_tag="VFG22_15545"
                     /inference="COORDINATES: protein motif:HMM:NF023385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET6417711.1"
                     /translation="QWESYEPRIHENVDRILEVLSRKDQRATFFCLGWIAERYPEVIR
                     TIHGLGHEIGSHSYAHNTLSDQNPEDFKRDVSRSLLALEDVIGEKVRCYRAPGFSLTV
                     ANVWAFDCLMELGIEVDCSLSTGSGFYGSFEILTSAQPLWIEHGGRRLKEFPLGAFPV
                     MGKRMIFSGGGYFRLLPYAAVRAMSRNSSYVMAYFHPRDFDPDQPVLSGLTIDRRFRS
                     YVGLSSSLAKLEQFLDDFTFVDIATALNTIDWDHAPLLRLPPPEQHRLA"
     gene            complement(822..1457)
                     /locus_tag="VFG22_15550"
     CDS             complement(822..1457)
                     /locus_tag="VFG22_15550"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6417712.1"
                     /translation="MNRRFQLFSQLIKPFSEKSRKRRMKIFAEVMALSPGTRILDLGG
                     QPGAWRNVVVPLDLTILNLPGVAEASVELPQHRVRYVEGDACNVVQFDRGDFDILHSN
                     SVIEHLGPEENQEAFASEVKRLSRRYWVQTSSEWFPIEPHCGMPFWWFYPEPARQWFI
                     RRWSQQVPRWAEMIAGTRLVTKKRLQSLFPETSSYVEWSYGFPKSYVVYQS"
     gene            complement(1576..2703)
                     /locus_tag="VFG22_15555"
     CDS             complement(1576..2703)
                     /locus_tag="VFG22_15555"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET6417713.1"
                     /translation="MDKAFLARSAVERLQAVSRETLYLHGGLDRTRPVVVQGMVTERC
                     NYRCEYCAFGRRESTADEISSEEWCAALLTLREFIGPFLIQFAGGEPFVKRGFLDILD
                     FCHEQGIHFGLITNGSGLSERNCRRLSNARPFNVDVSVDASRAEIHDAVRGVPGSLES
                     IEAGVGRLLEARRTVGAVFPVRIKTTVHRRNQGTLSDLVRWVEAIGATSVDFSPVRRW
                     TPEVEEDLWIRDSKSLQSLEREIAELIRLKRAGAPIETSEEKLSAFVDHFEDREGSLA
                     VSPCRVGMREYHIRPNGDVKVCWYHPAIGNVRRGDARSIWHGEKARAVRLNTVQCELF
                     KVHSCAHSCLGHQPIRKLVARTWMLMRHSGRGAARDARKEG"
     gene            2894..3181
                     /locus_tag="VFG22_15560"
     CDS             2894..3181
                     /locus_tag="VFG22_15560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6417714.1"
                     /translation="MQSILILLLALVAILEAGPDVARAPEPATRPNTLLIVWGDLDEF
                     LLVDNAHRLHERLPRSRLQVFERCGHFSYQDKPEEFAQMILDWVGGGYRGV"
     gene            complement(3283..3633)
                     /locus_tag="VFG22_15565"
     CDS             complement(3283..3633)
                     /locus_tag="VFG22_15565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417715.1"
                     /translation="MIILVGLTWCVFWMDNESLSSRVNITFIGILSVVAYYFVILDNV
                     PEVSYLTLVDAFIISTFLILAAGVILGVIMETLGHSGRTELGARVDRICRWAFPTAYL
                     FTTAALGIVFFNLY"
     gene            complement(3673..4173)
                     /locus_tag="VFG22_15570"
     CDS             complement(3673..4173)
                     /locus_tag="VFG22_15570"
                     /inference="COORDINATES: protein motif:HMM:NF014927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417716.1"
                     /translation="MDPRLAYDPAEYGMPEDWVPGDYSRAPRRIYQDNFAVNEAFQGW
                     RPHVVIPNGIGDRSTTNMAISVWPDGWVAYSETFYAKVEAPMDLRRFPFDVQDLEIFF
                     HPFIYQRDEIVLVPDDRLARTWNQNLGIADWSRESVLMKERSTEIAYFDDSRSAISEF
                     VVTLRI"
     gene            complement(4464..5009)
                     /locus_tag="VFG22_15575"
     CDS             complement(4464..5009)
                     /locus_tag="VFG22_15575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417717.1"
                     /translation="MIRSLHLAIPMIAVLVVAVANPERGLAEGSPIRALVGTFAYAGS
                     SERGRIMIDRAIELATEEMGFLRESIASDRLQEKTAIAPRIVIAQEKNMVTVEFQGAT
                     YRSRINGGWVTATSPGGESISVRHRIRGDKLVQDIRTQKGRRVNLFRARGKDHLNLAV
                     VISSPHLPEDVRYDLTYRRVE"
     gene            5308..6207
                     /locus_tag="VFG22_15580"
     CDS             5308..6207
                     /locus_tag="VFG22_15580"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6417718.1"
                     /translation="MLLDFARCPDCQGALRAEEGMALVCQHCSERYPIVDGIPQLVGQ
                     KSAINASEVETQDYVTDFYENLRYKLPWARAYHEHTLQQMLGLSNLKGRVLDNGCGNG
                     VFLERIAREGLPGTELIGTDISLGMLAHAKRRLSACGSSTTLVQADACRLPFAEDSFD
                     LVFARGLLHHLPDPGAGAREMARVLRAGGVVIALDPNRTLISELPRRLLRGSSHFDED
                     HKNFTAGQMREILSPYFRIDAVHHMGYVAYPLLGFPDITNFGKILPLNALAPVLMKLD
                     DGLAEVPGIRRLGWGLMVRAVVD"
     gene            complement(6169..>6937)
                     /locus_tag="VFG22_15585"
     CDS             complement(6169..>6937)
                     /locus_tag="VFG22_15585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6417719.1"
                     /translation="GYGGALKTGMRAASRPYVAWFDADNEHRVEDLERMVERIQSESL
                     AAVIGRRPKAGPSVVRNYGKSAIRMLARTLGVRLGKDLNCGLRVFRRSVILRYLSLLP
                     DSFSASMTSTMVLIEEGYPFAFEEIDVEERIGTSKVKLGDGFSTMALVLRMVMLFAPL
                     RIFLSFGGFFLLSGTAYGIVRAILSGRGLPVAGLLLIVTGVLLVMFGLVADQISRMRL
                     TQIGGPAPDDYEEVSNSPAERGEPALVDDGADHEAPT"
BASE COUNT         1433 a         2119 c         2015 g         1370 t
ORIGIN      
        1 gcagtgggaa tcgtacgagc cgcgtattca cgagaacgtc gaccgaatcc tcgaagtgct
       61 ctcacgcaaa gatcagagag caacgttctt ctgcctcggc tggatcgccg agcgctatcc
      121 ggaggtcatc cgcacaatcc acggcctcgg acacgagata ggatctcact cctacgcgca
      181 taacactctc tctgaccaga accccgagga cttcaaacgc gacgtttcgc gctcactatt
      241 ggcgttggaa gacgtgatag gagagaaagt gcgctgttat cgcgctccag gattctccct
      301 gacagttgca aacgtctggg cattcgactg tttgatggag cttggcatcg aggttgactg
      361 ctccttatcc accggcagtg ggttctacgg gagcttcgag atcctcacgt cggcacagcc
      421 cctttggatc gagcacggtg gccgacgcct caaggagttt ccgctcggcg cgttccccgt
      481 catgggaaag cgcatgatat tctcaggggg cgggtacttt aggctgctgc cctatgcagc
      541 cgtgcgagcc atgtcgcgaa attcgtctta cgtgatggcg tactttcatc cgcgtgactt
      601 cgatccagac cagccagtcc tttctgggct caccatcgat cgacggtttc ggtcctacgt
      661 agggctttcg tcgtccctcg ccaagttgga gcagttcttg gacgacttca cgttcgtgga
      721 catcgcaacg gcgttaaaca ccatcgactg ggaccacgcg ccacttcttc gccttccgcc
      781 gccggagcag catcgtctcg cttagcggca ctggagcggc ttcagctttg gtacacgaca
      841 tatgacttcg ggaatccgta cgaccattcg acgtatgacg aagtctctgg aaacagcgac
      901 tgaagccgct tcttggtgac gagacgggtc cctgcgatca tctctgccca cctgggcacc
      961 tgctggctcc agcgccgaat gaaccattgc cgcgcgggct cggggtagaa ccaccaaaac
     1021 ggcattccgc agtgtggctc aatcgggaac cactcggagg atgtctgaac ccagtagcga
     1081 cgactcagcc gcttcacttc ggaagcaaag gcctcttggt tttcctcggg ccccaggtgc
     1141 tcgatcacgc tgttgctgtg aaggatatcg aagtctcctc tatcgaactg caccacgttg
     1201 caggcgtctc cctcgacata gcggacacgg tgctgcggca gctcgacgct cgcctctgcg
     1261 actccaggca gattgaggat cgtgagatcg agcggaacaa ccacgttcct ccaagctccg
     1321 ggctgtccac ccagatcgag aatacgagtt cccggggaca gtgccatgac ctcggcaaag
     1381 atcttcattc ggcgcttccg agacttctcg gagaatggct tgatgagctg cgagaacagc
     1441 tggaaacgcc ggttcattgt gacaaatatg ccttcctgag cgcgtgccga caaatcgcgg
     1501 tcgaaccacg cccctcgagc gaagcggtcg ggtgctcgtc atcgctggtc taggagaggc
     1561 ccaggactcg caggcttacc cttctttgcg cgcgtctcgc gctgcgccgc gtccactgtg
     1621 tcgcatcaac atccaggtcc tcgctaccag cttccgaatc ggctggtgtc cgaggcagga
     1681 atgagcgcag gaatgaacct tgaaaagctc gcattgcacc gtgtttagac ggaccgcacg
     1741 cgccttttcg ccgtgccaaa tcgacctcgc atctccgcga cgaacgttgc caatcgcggg
     1801 atgataccaa cacaccttca cgtccccatt ggggcgaatg tgatactccc gcattccaac
     1861 tcggcagggc gagacggcga gcgacccctc acggtcctcg aaatgatcga cgaatgcaga
     1921 gagcttctcc tcggatgtct cgataggcgc cccggcccgc ttcaagcgaa tgagctccgc
     1981 gatctccctt tccagagact gcaagctctt ggagtcgcga atccaaagat cctcttcgac
     2041 ctccggcgtc caccgccgga ctggactgaa gtccacactc gtcgcgccga tcgcttccac
     2101 ccaacgcact agatcggata gcgtcccctg gtttcttcga tggaccgtag tcttaatgcg
     2161 aacgggaaag accgcgccaa ccgtgcgccg cgcctcaagc agccgaccga ctccagcctc
     2221 gatgctctca agcgacccgg gtacgccgcg cacggcgtca tggatttccg cccgcgaggc
     2281 atcgaccgaa acgtcgacgt tgaacgggcg cgcgttgctc aaccgtctac aattccgctc
     2341 gctcagtccc gagccgttgg tgatgagtcc aaaatggatc ccttgttcgt gacagaaatc
     2401 gaggatgtcg agaaagccac gcttgacgaa cggctctcct cccgcgaact ggatgaggaa
     2461 gggtccgatg aactcgcgaa gcgtcaggag cgcggcgcac cattcctcgc tcgagatctc
     2521 gtcggcggtc gactccctac gcccaaacgc acagtactcg cagcggtagt tgcagcgttc
     2581 tgtaaccatc ccctgcacga cgactggacg tgtccggtcg aggcctccgt gaagatagag
     2641 agtctctcga gacaccgcct ggagacgctc gactgccgac ctcgcaagaa atgctttgtc
     2701 catgagattt cttcctgtaa cgaacgcgac gcgggcgcgc gctcgcgccc aggtgctcat
     2761 tcgtactcag actgccaacc taacgtcaag gccgagtcag agcacagccg aaagcggata
     2821 gattcctcat tcacgactac cgcaaaatcg cgtatccgct agagtgctgc cgcgaacacc
     2881 gagcgggatg ccaatgcaat caattctcat tttgttactc gcgctcgtcg cgatcctcga
     2941 agctgggccc gatgttgccc gcgcccccga accggcgaca cgaccgaaca ccctactgat
     3001 cgtctggggc gatctcgacg agttcctctt ggtggacaat gcccatcgac tgcatgagcg
     3061 cctcccgcgc agtcggcttc aggtgttcga gcgctgcggc cacttctcct accaggacaa
     3121 gcccgaggag tttgcgcaga tgatcctcga ctgggtgggc ggcggatacc gaggggtttg
     3181 agtggagaaa gcgccttgct cgccgtgctg agccgcgagg tatgaagggt ttgcggcggc
     3241 tctgctgggg ctacccgtcg ccgcagtggc caccattctc gctcaataca gattgaagaa
     3301 gacgattccg agagccgcgg tggtgaacaa gtaggccgtc ggaaacgccc agcggcagat
     3361 tctatccacc ctggcgccga gctctgtacg gccagagtgg cccaaggttt ccatgatcac
     3421 gccgagtatg acgcccgcag cgaggatcag gaaagtggag atgatgaagg catcgacgag
     3481 cgtcagataa ctgacctccg ggacgttatc gaggatgaca aaatagtacg caacaacgct
     3541 caatataccg atgaacgtga tgttgacgcg gctagacagc gactcgttgt ccatccagaa
     3601 aacgcaccat gtcaggccga cgagtatgat catcgggaag atgatgctga tcaggaagtg
     3661 cgtcggcctg cgctagatcc gcaaggtgac cacgaattcg gatatcgccg aacgcgaatc
     3721 gtcgaagtag gcaatttcgg tcgacctttc tttcatgagg acggactctc gtgaccagtc
     3781 agcaatcccg aggttctggt tccaggtgcg cgcaagcctg tcgtcgggga ccagcacgat
     3841 ctcatcccgc tggtagatga atggatgaaa gaagatctct agatcctgta catcgaatgg
     3901 gaatcgccgt aaatccatcg gcgcttctac tttggcatag aacgtctctg agtaggcgac
     3961 ccaaccatca ggccatacgc tgatcgccat attcgtggtc gaccgatctc caataccgtt
     4021 gggtatcacg acatgtggac gccatccctg aaaggcttcg ttcactgcga agttgtcctg
     4081 gtagatacgc cgcggcgctc tcgaataatc cccgggaacc caatcctccg gcataccgta
     4141 ctcggcgggg tcgtacgcca gcctcgggtc catccattct gggcatgagc ggagagcgcc
     4201 accacacaga cgagaaagcc gactagagat tctattcgca tgcagagccc cgagaatcct
     4261 ccggggacgt ttagtgaacg acgctcgcct tgtcgagggc cggaacgagc ggttcgtcga
     4321 cgtgccgttc aaagacggca aggtcgtgtc ggagcatctg cggataggcg aaacaaaggt
     4381 ggtcttgggt ccaccgcagc tcggcatagc gagctagcgc cgcgaaattc ggactcccga
     4441 ctgggcccaa tgagctaaga aagctattcc acccttctgt acgtcaagtc gtagcgaaca
     4501 tcttcgggaa gatgagggct cgaaatgacg acggcgaggt tcaagtggtc cttgccccgg
     4561 gcgcggaaga gattgacgcg ccgccccttt tgggtgcgaa tatcctggac gagcttgtca
     4621 ccccgaattc ggtgacgaac actgatcgac tcgcctccgg ggctcgtcgc agtgacccag
     4681 ccgccattga tccgactgcg atacgtcgcg ccctgaaact cgactgtgac catgttcttc
     4741 tcttgagcga tgacgatacg cggtgcgatc gcggtcttct cttggaggcg atccgacgca
     4801 atagattctc tgagaaagcc catttcttcg gtcgccagct cgatcgcgcg gtcgatcatg
     4861 atgcggccgc gctccgacga gcccgcatat gcgaacgtgc ccacgagagc ccttatgggt
     4921 gaaccctccg caagaccgcg ctctgggttg gcgaccgcca ccaccaagac ggcaatcatg
     4981 ggaatcgcga ggtggagcga tcggatcaat ctggctcaac ctcgctctcc aaacgagatc
     5041 gcgcaagcca ttgttgtgcg ttcggacggc tccagcgggc ggataggcga aacaaaggcg
     5101 gtcttgggtg caccgcaacg cgacataacg agccagtacc gcgaaattcg gctctcagtt
     5161 tgatcccgat gggcgagccc agtgaagtct gctcccaggt gcgctgattc atcttgtatg
     5221 attcaggccc cacagccggc gcggttggac ggattgttcg cgcgacgacg cggcgctacg
     5281 gttggcgtgt aggaatgaaa ggtagagatg ctattggatt tcgcccgatg tcctgactgc
     5341 cagggggctc tcagggccga agagggcatg gcactcgttt gccagcattg ctcggagcgg
     5401 tacccgatcg tcgatggcat tcctcagctg gtcggccaga agagcgcgat caatgcgagc
     5461 gaggtcgaaa cccaggacta cgtaacggac ttctacgaaa acctgcggta caagctgccc
     5521 tgggcgcgcg cctatcatga gcacactctt cagcagatgc ttgggctctc caacttgaag
     5581 ggcagagtgc tcgacaacgg ctgtgggaat ggagtgttct tggagcggat cgcacgcgag
     5641 ggcctgccgg gcaccgaatt gatcggcacc gatatcagct tggggatgct tgcccacgcc
     5701 aagcgtcgac tgagcgcttg tgggtcctcc accacgctgg tgcaggccga cgcgtgtcgt
     5761 ttgccctttg cggaggacag cttcgatctc gtcttcgcgc gtggcttgct tcaccactta
     5821 cccgaccctg gggccggcgc gcgcgagatg gctcgagtcc ttcgcgcggg cggggtcgtc
     5881 atcgctctcg accccaaccg gacgctgatc tccgaactcc cgcgtcgtct cttgcgcgga
     5941 tcttctcact tcgacgagga ccacaagaac ttcaccgcag gtcagatgcg cgagattctc
     6001 tcaccctact ttcggatcga tgcggttcac cacatgggtt acgtggcata cccgctgctc
     6061 ggatttcccg acatcacaaa ctttggcaaa attctgccgc tgaacgcgct ggcgccggtt
     6121 ctgatgaaac tggatgacgg gctggcagag gttccaggga tacgccgtct aggttggggc
     6181 ctcatggtcc gcgccgtcgt cgactagtgc gggctcgcct cgttcggccg gtgaattcga
     6241 cacctcctcg tagtcatcgg gggcaggccc gccgatctgc gtgagacgca tacggctgat
     6301 ctggtccgca acgaggccaa acatgacgag caggacgccg gtaacgatga gcaggagacc
     6361 agccaccggg agaccccgtc cgctgagaat cgcacgcacg atgccgtagg ccgtcccact
     6421 caggaggaag aagcctccga aactcaggaa gatacggagt ggtgcgaaca gcatcaccat
     6481 tcggagaacc agcgccatcg tcgagaagcc gtcgccaagc ttcaccttgc tcgtgccgat
     6541 gcgctcttcg acgtcgatct cttcgaatgc gaacgggtac ccctcttcga tgaggaccat
     6601 cgtgctcgtc atgctggcac taaacgaatc gggaagaagc gaaaggtagc ggaggatgac
     6661 cgacctgcga aacactcgga ggccgcaatt gaggtccttg ccgaggcgga cgcccagcgt
     6721 tcgggccagc atgcggatcg ccgattttcc atagttgcgg acaaccgaag gcccggcttt
     6781 cggtctccgg ccgattacgg cggcgaggga ttcgctctgg attcgctcga ccatgcgctc
     6841 gagatcctcg acgcgatgct cgttgtcggc atcgaaccat gccacgtacg gacgcgaggc
     6901 ggccctcatc ccggtcttca acgcacctcc gtagcca
//
LOCUS       DASRZF010000350         3486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2675521, whole genome shotgun sequence.
ACCESSION   DASRZF010000350 DASRZF010000000
VERSION     DASRZF010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2675521"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VFG22_15590"
     CDS             complement(<1..449)
                     /locus_tag="VFG22_15590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417720.1"
                     /translation="MSIPITRQERQFNRFAWAIRMSPLLVAIILATLWIAGTPPEVPD
                     LRLSAPLVAQPGTSIGLRAWQLAEDENGSPVILAPGVLVELQNEVGLVLATTELVESL
                     VQGREGRLSIPAGVDEVLTLVAHAEIDGRDVSVERALYVQEGIESRLP"
     gene            493..963
                     /locus_tag="VFG22_15595"
     CDS             493..963
                     /locus_tag="VFG22_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HET6417721.1"
                     /translation="MGATAEHDVEQLRARLNDYMTKHGLRSTEQRRLVTEMFFASSDH
                     LSIEDLLDKVRVEEPKIGYATVYRTLKLLKECGLAFERHFGDGVSRYEVAWADEHHDH
                     LICVECEKIIEFEDEGIEELQHKVAARKGFKLVRHKLELYGFCADCQAKNARAK"
     gene            complement(991..2832)
                     /locus_tag="VFG22_15600"
     CDS             complement(991..2832)
                     /locus_tag="VFG22_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HET6417722.1"
                     /translation="MAQAINRYRADLRDFRFLFFEQFDLPAVLGKEPYSEWSADECNM
                     VLDEVYRFVCDVTGPLNGTGDAQGCRIEDGRVITPDGFKEAWDKTWEAGWRTIASPSR
                     WGGQDAPNMIGSFVEEMMCGANTAFTMYTGLTLTAAELIMEFGTEEQKQKYVPKMMSG
                     EWTGTMCLTEPHAGSDVGSASSGATRNDDGTYNVRGTKIFISAGDNDLGTNVIHLVLA
                     RIEGAEAGTKGLSLFLVPRVRINEDGSLGELNDVHVPSIEHKMGINASATCVMQFGDE
                     DACIGEVVGGVEHQGIRQMFHMMNAARIGVGIQGLSVASAAYLSALEYARERKQGASI
                     KQFKDANAPRVPILDHPNIRRDLLAMKARVEGIRALVMKLSSHGDRLRATQGKDDESE
                     AYHKGQVDLLTPLVKAYGTDAAFEVSSRAIQVYGGHGFLKDHPVEQYCRDSKIFQIYE
                     GTNFIQSMDLVSRKLGQAGGANTQAFLMDIQNFITENKDTEELKQGIGHLQAAHEAVA
                     ASVMQFIGWFKGGKLEHIPLGAEDFLTIMSDLAVGWLLLDQASIALKKLPETSETHPD
                     HAFYAGKKFSAQYFANNVLSTLPSRVQRLSEIDTLPVEIPDEAFATI"
     gene            complement(2924..>3486)
                     /locus_tag="VFG22_15605"
     CDS             complement(2924..>3486)
                     /locus_tag="VFG22_15605"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HET6417723.1"
                     /translation="RVYCWLIEELGIHPDHIVIGGESAGAGLTLSTLVSLRDAGLPLP
                     AGAVLVSGWFDLDSRSTSMQFNSHYDFVTRIGIRTMAKRFAPRSEHGNPLVSPVHADM
                     SGLPALLIQVGAAETLLDDNLREAEAARSAGVEVKLEVWPDMIHAWHVFAPMLEEGRR
                     AIARIGEFVKERTALADPAGLDATRL"
BASE COUNT          678 a         1064 c         1068 g          676 t
ORIGIN      
        1 ggaaggcgag attcgatccc ctcctgcaca tagagggcgc gctcgaccga gacgtcgcgt
       61 ccgtcgattt ctgcgtgtgc gaccaaggtc agcacctcgt cgaccccggc gggaatgctc
      121 agccggcctt cacgtccctg cacgagggac tccacgagct ctgtcgtcgc aaggacgagg
      181 ccgacttcgt tttggagctc gaccagcacg ccaggtgcca ggatgaccgg agatccgttc
      241 tcgtcttcgg ccagttgcca cgcacggagc ccaatgctgg ttccgggttg cgcgacgagc
      301 ggcgcactca aacgaagatc gggaacctct gggggggtcc ccgcgatcca gagcgttgcg
      361 aggataatcg cgacgagcaa cggcgacatt cggatcgccc aggcgaatcg gttgaattgt
      421 cgttcctgtc gggtgatggg gatggacaag ttcgagatct taccttagga acggtgggga
      481 tatagagcgc gaatgggagc caccgccgaa cacgacgtcg agcaactccg tgctcgcttg
      541 aacgactaca tgaccaagca cggtctgcgg tcgaccgagc agcgccgcct cgtcaccgag
      601 atgttcttcg cgagcagcga tcacctgtcg atcgaggacc ttctcgacaa ggtgcgcgtc
      661 gaggagccca agatcggcta cgccaccgtg taccgcacgc tcaagctctt gaaggagtgc
      721 gggctcgcat tcgagcgaca ctttggagac ggcgtatcac gctacgaggt cgcctgggcc
      781 gacgagcatc acgaccatct gatctgcgtc gagtgcgaaa agatcatcga attcgaggac
      841 gaggggatcg aagagctgca gcacaaagta gccgctcgca agggtttcaa gctggtgcgg
      901 cacaagctcg agctctacgg cttctgcgcg gactgccaag ccaagaacgc gagagccaag
      961 tagacccggc agtccggagt cgtcatacgc tcagatggtg gcaaacgctt cgtccgggat
     1021 ttcgacggga agcgtatcga tttcactcag tcgttgcacg cggctcggca aggtcgagag
     1081 cacgttgttc gcgaagtact gcgcagagaa cttcttgccc gcgtaaaaag catggtccgg
     1141 gtgggtctcc gaggtttccg gaagcttctt cagcgcaatg gatgcctggt cgagcagcaa
     1201 ccagcctacg gccagatcgc tcatgatggt gaggaagtcc tctgcgccga gcggaatgtg
     1261 ctccaacttg ccgcccttga accatccgat gaactgcatg accgacgcag ccaccgcttc
     1321 gtgcgcggcc tggaggtggc cgatgccctg cttcagctcc tcggtgtctt tgttctccgt
     1381 gatgaaattc tggatgtcca tcaggaacgc ctgagtgttg gctccgccgg cctggccgag
     1441 cttccggctc acgaggtcca tcgattgaat gaaattcgtg ccctcgtaga tctggaatat
     1501 cttggaatcg cggcagtact gctcgaccgg atggtccttc aggaagccgt ggccgccata
     1561 aacctggatg gcgcggctcg acacctcgaa cgccgcgtcg gtgccgtagg ccttcacgag
     1621 tggggtgaga agatccacct ggcccttgtg gtaggcctcg gactcgtcgt ccttgccctg
     1681 cgtcgcacgg agacgatcgc catggctcga gagcttcatc actagcgcgc gaatcccctc
     1741 gacccgcgcc ttcattgcaa gaaggtcccg acgaatgttc gggtggtcga ggatgggaac
     1801 gcggggagcg ttcgcgtcct tgaattgctt gatgctcgcg ccctgcttgc gctcgcgggc
     1861 gtactcgagc gcactcaggt aagcggcgga ggctacggat agcccttgaa tcccaacccc
     1921 gatgcgcgcg gcgttcatca tgtggaacat ctgacgaatg ccttggtgct ccacaccgcc
     1981 gaccacttca ccgatgcatg cgtcctcgtc accgaactgc atcacgcaag tagccgaggc
     2041 gttgatgccc attttgtgct cgatcgaagg aacatggacg tcgttgagct ccccgagcga
     2101 cccgtcttcg ttgatccgta cccgcggcac gagaaagagc gaaagcccct tcgtgccagc
     2161 ctccgcacct tcgatgcgcg cgagcacgag atgaatcacg ttggtgccga ggtcgttgtc
     2221 tccggcactg atgaagatct tggtgcctcg gacattgtac gtgccgtcgt cgttgcgcgt
     2281 ggcaccgctc gaggcggagc caacgtcgga gccggcgtgc ggctcggtga gacacatggt
     2341 cccggtccac tcaccgctca tcattttggg aacgtacttc tgcttctgct cctcggtccc
     2401 gaactccatg atgagctctg ctgcggtgag cgtgaggccg gtgtacatcg tgaacgcggt
     2461 gttcgcgccg cacatcatct cctcgacgaa ggagccgatc atgttcggtg cgtcttggcc
     2521 gccccagcgc gaaggcgagg caatcgttcg ccagcccgct tcccaggtct tgtcccaagc
     2581 ctctttgaaa ccatcgggcg tgatgacgcg accgtcctcg attcggcagc cttgcgcatc
     2641 tcccgttccg ttgagcgggc cggtcacatc gcagacgaac cgataaacct cgtcgagcac
     2701 catgttgcac tcgtcagcgc tccactcgga gtacggctct tttccaagaa ctgcgggcag
     2761 atcgaactgc tcgaagaaca gaaagcgaaa gtcccgtaga tccgcacgat agcgattgat
     2821 tgcttgcgcc acgtcagcct ccagtgttcg aaccggtcac cctcgagctt cgcttgatta
     2881 cgggatgaag ccctgatgga tgccagctgt aagaaagctg aggtcagagc cgcgttgcgt
     2941 caagcccggc agggtccgca agtgctgtgc gttccttcac aaattcgccg atccgggcga
     3001 tcgcgcgacg cccctcttcg agcatgggag cgaacacatg ccaggcgtgg atcatgtcgg
     3061 gccagacctc gagcttgacc tcgacacctg ctgaacgcgc cgcctccgct tcgcggaggt
     3121 tgtcgtctag caaggtttct gcagctccaa cttggatcag caacgcgggc aagccactca
     3181 tgtccgcatg cacgggtgac accaaagggt tgccgtgctc ggacctcggg gcaaagcgtt
     3241 tggccatcgt ccggattccg attcgggtga cgaaatcgta gtggctgttg aactgcatgg
     3301 aggtgctgcg ggaatccagg tcgaaccagc ccgagaccaa tactgcgccg gcaggcaaag
     3361 ggaggcccgc gtcgcgcagc gaaaccaagg tggatagcgt gaggcctgcg cccgccgact
     3421 cgccgccgat gacgatgtgg tccggatgaa tcccaagctc ctcgatcagc cagcaataga
     3481 cgcgat
//
LOCUS       DASRZF010000351         2013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_265121, whole genome shotgun sequence.
ACCESSION   DASRZF010000351 DASRZF010000000
VERSION     DASRZF010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265121"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            291..1166
                     /locus_tag="VFG22_15610"
     CDS             291..1166
                     /locus_tag="VFG22_15610"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6417724.1"
                     /translation="MSISIEQWRERGHVARLMDRKIFCVDSGELDKPVIFLIHGFPTA
                     SWDWERIWPLLNESHRLVALDMLGFGFSDKPNPHEYSIMEQADLCEALIAERKLAKFH
                     VLAHDYGDTVAQELLARQNEGSGEGTWLSVCFLNGGLFPETHRARLIQKLLLSRLGPL
                     INQLTTKRTFDKSFSAVFGPDTKPSSAQLESFWNLINYNGGRHCFHNLISYMSDRKKH
                     RQRWVRALQDSRAPIGLINGSIDPVSGAHMVSRYREVVSRDHFTVELPRVGHYPQVEA
                     PAEVAHAYFDFLRRL"
     gene            complement(1163..>2013)
                     /locus_tag="VFG22_15615"
     CDS             complement(1163..>2013)
                     /locus_tag="VFG22_15615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417725.1"
                     /translation="VATRAGESWRQMAITEPGVGTAWEPDVAIDPVTGNYHVTWLDLR
                     NGGRPKPWVARSEDGESWETRQVDPGNAVVDNPRGDAAFPKISARDGSVFVAFSDFRE
                     FSWDVYLSASGDGGLTFAPATRINPRAKDVIPVPSADPIESERIHGDIAIALDSSGRP
                     SVAWTERQDRRYESRIRVWREGETARVDDAPGTIDAWRPSLATDGLGQVLTVWQDMRE
                     GTNQIRFAQTAGPSLEPNASVRIDDAAPATHLYAPQIANHGEQILVAWEDPRSGYARV
                     RISASD"
BASE COUNT          380 a          655 c          571 g          407 t
ORIGIN      
        1 cctctgccag ttcaattggg aatcgcttca aatacgtgag gtccgacgtg gcgcggtgaa
       61 gtgccgttac gttccagcct tggttcacga ggcgctcgat cagattgatc ccaatgaacc
      121 cggtgccccc ggtcacgaat gcagattgcg ccatccttga tgtccttcgt tcggtcgcga
      181 tactctccag cacttcgcgg cgctgtcaat gagaaccgcg cgtccaatga ctccatggtt
      241 cgctcacgct cgtgctattc gcggcgatct cgtttagcct cggatccagc atgtcgattt
      301 cgatcgagca atggcgcgaa cgcgggcacg tcgcccgctt gatggaccgc aagatctttt
      361 gcgtcgattc cggcgagctc gacaaacccg tgatctttct catccacggg tttcctaccg
      421 cttcctggga ttgggaacgc atctggccgt tgctcaacga atcccaccgc ttggtggctc
      481 tcgacatgct cggattcggt ttcagcgaca agccgaaccc gcacgagtac agcatcatgg
      541 agcaggcgga cctctgcgaa gccctgatcg ccgaacgcaa gctcgcgaag tttcatgttc
      601 tcgcacacga ctacggcgac acggtcgcgc aggagttgct agccaggcag aacgaaggaa
      661 gcggcgaggg tacttggctg tcagtctgct ttctcaacgg aggcctcttc cctgagacgc
      721 atcgggcacg cttgatccag aagcttcttc tgagccgcct cggcccgctc atcaaccagc
      781 tgaccacgaa gcgcacattc gacaagtcgt tcagtgcggt cttcggcccg gacacgaagc
      841 catcctccgc gcagctcgag agcttctgga acttgatcaa ctacaacggc ggaaggcact
      901 gctttcacaa tctgatcagc tacatgagcg accggaagaa gcatcgccaa cgatgggtcc
      961 gtgccctaca agacagtcga gctccaatcg gcttgatcaa cggatcgatc gacccagttt
     1021 cgggcgcgca catggtctcg cgataccgcg aggtagtcag tcgagatcac ttcaccgtgg
     1081 agctcccgcg cgttggacac taccctcagg tagaggctcc cgccgaggtg gctcacgcat
     1141 acttcgattt cttacgccgg ctctagtcgc tcgccgaaat gcgcactcga gcgtatccgg
     1201 aacgcggatc ctcccaagcc acgagaattt gctcgccgtg gttcgcgatc tgtggggcgt
     1261 agagatgggt agccggcgca gcatcgtcga tacgcacact cgcgttgggc tcgagcgagg
     1321 gtcccgcagt ttgagcgaag cggatctgat tcgtgccctc gcgcatgtct tgccacaccg
     1381 tgagcacctg gcccaggccg tccgtagcca gagacggacg ccaagcatcg atggtgccgg
     1441 gcgcatcgtc gacccttgcc gtttcgcctt cgcgccacac tcgaatccgg ctctcgtagc
     1501 gccggtcttg ccgctccgtc catgcgacgc tcggccgccc agacgagtcg agtgcgatgg
     1561 ctatgtctcc atggatacgc tcggattcga tcgggtcagc gctcggaacc gggatgacat
     1621 ccttcgcgcg gggattgatt cgagttgctg gcgcaaacgt caatccacca tccccgctcg
     1681 ccgacagata gacgtcccaa ctgaactccc taaaatccga gaacgccacg aacaccgaac
     1741 catcgcgtgc cgagatctta ggaaacgcgg cgtctccccg cgggttgtcg acgacggcgt
     1801 tgccggggtc gacctggcgc gtctcccagc tttccccgtc ctcactccgg gcaacccaag
     1861 gcttcggtcg tccaccgttg cgaaggtcga gccaggtcac gtggtaattg cccgtgaccg
     1921 ggtcaatcgc cacgtccggc tcccaggctg tgccaacacc cggctcggtg atcgccattt
     1981 gcctccagga ttctccggca cgggttgcga cta
//
LOCUS       DASRZF010000352         2775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4735897, whole genome shotgun sequence.
ACCESSION   DASRZF010000352 DASRZF010000000
VERSION     DASRZF010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4735897"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VFG22_15620"
     CDS             complement(<1..525)
                     /locus_tag="VFG22_15620"
                     /inference="COORDINATES: protein motif:HMM:NF025597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HET6417726.1"
                     /translation="MVRKSGAPEWHVSINDVPVGPVHLEEMGHKIDAGAVSEYSLVWR
                     EGFEEWRPLATVPALMALLHERRHSGPPPRSSFSSMPPFVDSRTSVDDSVAFAATVAA
                     AEARSSSGMAMGMDFTPLADSLQPEGSEPDMFREMSQPPAPLGPSDVFGSSPQLGYFS
                     GFPAGPDETVAVPSA"
     gene            complement(1029..1490)
                     /locus_tag="VFG22_15625"
     CDS             complement(1029..1490)
                     /locus_tag="VFG22_15625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417727.1"
                     /translation="MALSASLLAAVGCTKNIPNTTVKDTPENRAVITFMENYRNAVES
                     RDVGALLAMAHPQYLDDNGTPTGDDDLDYRALQRKLSRWRERVTDVRYEIKYRTITRE
                     MDRVMIAYRYSASFRIAYDEEDQRWSRRIGENRLVLLYDDIERRYYVLSGM"
     gene            complement(1573..>2775)
                     /locus_tag="VFG22_15630"
     CDS             complement(1573..>2775)
                     /locus_tag="VFG22_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6417728.1"
                     /translation="VRMSGMTGIELCAELKARSSLATVILMSAYGSVDLAIDAMKAGA
                     YDYISKPFKQDEVLLALTKAEERESLRRENRALKQAIRKEQTFHGILGKSEAIEKVFA
                     TIGKVADYKTTVLIQGESGTGKELVARALHDGGPRKNKPFVPLNCGAIPETLLESELF
                     GHKRGAFTDAHADKKGLFAEAHQGTLFLDEIGELPLTLQVKLLRVLQEGRMRPLGTAQ
                     DQEVDVRVIAATVRDLRREVEENRFREDLFYRLNVLQINVPPLRDRRDDIMLLVEHFI
                     ERNNLRLGTKIRDIDQRTRKLLLNYPWPGNVRELENTIERAVVLAEGETITVLDLPER
                     LREPADPVAASLSTGELSIKKTSRFMEETLIRRALEKTGGNRTSASKLLEISHRALLY
                     KIKDYGIQ"
BASE COUNT          520 a          864 c          832 g          559 t
ORIGIN      
        1 cgcggagggc acggcgacgg tctcgtccgg ccctgccggg aagcctgaaa aataaccgag
       61 ctgaggcgaa gagccaaaga catcggatgg gccgaggggc gccggcggct gcgacatctc
      121 tctaaacatg tccggctccg agccttcggg ttggagcgaa tcggcaagcg gggtgaagtc
      181 catgcccatg gccatgccag agctcgagcg agcttcagcc gccgcgaccg ttgcagcaaa
      241 ggccacggag tcgtcgaccg aggtgcgcga atcgacgaac ggtggcatcg aggagaacga
      301 gctacgcgga ggtggacccg agtgtcggcg ttcgtgcaac aaggccatca gcgccggtac
      361 cgtcgcaagc gggcgccact cctcgaaacc ctcacgccaa accagcgagt actcgctcac
      421 tgcgccggcg tcgatcttgt gtcccatttc ttcgagatga accggaccca ccggaacgtc
      481 gttgatcgaa acgtgccact cgggagcgcc gctttttctc accacaggtc cggtggcaac
      541 cggtactggt ggcgtcaagc cgggatgctg accaacgaac gcactgcttg ccgcagccgg
      601 taccgaggac agtcttcgcg actgcgcgga cgggcggcgc ggaggcaccg acggtggacg
      661 cctgcgaact ggtgacgagg ttcgccgtgt gcgcgcggtt ggcgcttcgt gcccaaaggg
      721 cgacaccggc tctgaggttc gcgccacctc ttgaaagcca tatgctgcgc cagccggaac
      781 agtgacatcg ggatcacctc gacggccgtc gatcagaatg ttgtgattgc acttccgaca
      841 cgggaagcga acgacgcgac cccgaacttt ttcgtccgag atcttgtact tggcgtggca
      901 gctatcgcac tcgaatctca tgggcttctt tgcttgtggg cagggacact cagctgcgcc
      961 ctttccagct tgtcatcgac ccgatcgtag cgcgaattcg catgtgtgtt caataagaac
     1021 gcaaaatttc acataccaga gagcacatag taccggcgtt caatgtcatc ataaaggagg
     1081 accagccggt tctcaccgat gcgacgcgac cagcgctgat cctcttcatc ataggcgatc
     1141 cggaagctgg cgctgtaccg gtaggcgatc atcacacggt ccatttccct agtgattgtg
     1201 cggtacttga tctcataacg aacgtcggtg acccgctccc gccaacgtga gagcttcctt
     1261 tggagggccc tgtagtcgag gtcgtcatca cccgtagggg tgccattgtc atccaggtac
     1321 tgggggtgcg ccatcgccag aagcgctccc acatccctcg attccaccgc gttccggtaa
     1381 ttctccatga aggtgatcac cgcccggttc tccggggtgt ccttcacggt cgtattcggg
     1441 atgttcttgg tgcaaccgac cgccgcaagg agcgatgcag acaacgccat cgtggccagg
     1501 gccctacgca tacagcacat cacgagatca acgctcctct ttcccaggcc attcccgatt
     1561 atccactggc ggctactgaa tcccataatc tttgattttg taaaggagag cgcggtggct
     1621 gatctcgagc aatttcgagg ccgaggtccg gttgccgccg gtcttctcga gagcccgccg
     1681 aatcagcgtt tcctccatga aacgcgaggt tttcttgatg gagagctccc cagtcgagag
     1741 gctcgcggcg accggatcgg ccggctcccg caatcgctcg ggcaggtcga gcacggtgat
     1801 cgtctcgccc tccgccaaca ccacggcacg ctcgatcgtg ttctcgagct cgcgcacgtt
     1861 accgggccag gggtagttga gcagcagctt gcgggtgcgc tggtcgatgt cgcggatctt
     1921 ggtgccgagc cgcaggttgt tccgctcgat gaagtgctcg acgagaagca tgatgtcatc
     1981 gcgtcgatcg cggagcggcg gcacgttgat ctgcagcacg ttcaagcgat agaagaggtc
     2041 ctctcgaaat cggttctcct ctacctcccg gcggaggtcg cgtaccgtcg ccgcaattac
     2101 gcgcacgtcg acttcttgat cttgggcggt cccaagaggc cgcatgcgac cctcttgcag
     2161 cacccgtaag agcttcactt gtagcgtaag cggcagctct ccgatctcgt ctaggaagag
     2221 cgtgccctga tgcgcctcgg cgaagagccc cttcttgtcg gcatgcgcat ccgtaaaagc
     2281 gcctctctta tggccgaaca gctcactctc taggagggtc tccgggatcg cgccgcagtt
     2341 gagcggaacg aacggcttat tcttacgcgg gccgccatcg tgaagcgcac gcgccacgag
     2401 ctctttaccg gtcccgctct cgccttggat caaaaccgtc gtcttatagt cggcgacctt
     2461 gccgatggtc gcgaacactt tttcgatcgc ctcgctcttg ccgaggatcc cgtggaaggt
     2521 ctgctccttg cggatcgcct gtttgagcgc acggttctca cggcggagcg attcccgttc
     2581 ttcggctttc gtcagcgcga gcagaacttc gtcctgcttg aagggcttgg agatgtagtc
     2641 gtacgccccg gctttcatgg cgtcgatcgc gaggtccacc gacccgtaag cgctcatcag
     2701 gatcacggtc gccagggacg agcgggcctt gagctccgcg caaagctcga ttcccgtcat
     2761 tccgctcatg cgaac
//
LOCUS       DASRZF010000353        11025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4397239, whole genome shotgun sequence.
ACCESSION   DASRZF010000353 DASRZF010000000
VERSION     DASRZF010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 11025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11025
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4397239"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="VFG22_15635"
     CDS             complement(<1..786)
                     /locus_tag="VFG22_15635"
                     /inference="COORDINATES: protein motif:HMM:NF042462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbnP family copper-binding protein"
                     /protein_id="HET6417729.1"
                     /translation="MKKSMILVAALASAGFGCDSSSGGDASQSVAIAFEAMVGSEVFV
                     CGTTYDNLGADDSSIEVSDFRFYVQDIELRNADGDYVAVELDTDNNIWQVDNVALLDF
                     EEGCTNFGDAGTNRVVTGTVPEGSYDGLRFKMGVPFELNHANPATAEPPLNLTSMEWN
                     WQGGYKFLRIDTGDSSETTWRMHLGSTGCDGDPFAGGTTTCAAPNRVDVELDPFDPES
                     DTVVADFAQLVAGASLDVNQPETPVGCMAGPTDGDCAALFENLG"
     gene            complement(807..1535)
                     /locus_tag="VFG22_15640"
     CDS             complement(807..1535)
                     /locus_tag="VFG22_15640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417730.1"
                     /translation="MTSRLLTVIVLLSAAALPQCMDVTDGIEVDVAYQPAATTAAIRT
                     DMGYAVRLERALIAVGKVELIRCDNYVADLWNLIVPARARAHELSTPTSLGVPLIIDL
                     MESAGVPLFAGTLRPPPGRYCGIRVLGMPADQDTEGLTAETLDMMHHSVLISGRVEDE
                     STGEETPLLARISEILECEMLFDRPLVFEGPVLESVSIQIDQRTWFDGIDFAPQDSVA
                     VHQQITENVRSSLKAALSNWGADL"
     gene            complement(1525..2415)
                     /locus_tag="VFG22_15645"
     CDS             complement(1525..2415)
                     /locus_tag="VFG22_15645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417731.1"
                     /translation="MKGGWVIAALAASVWFAAKAYACGTCAVGDPTLTVVGMEQPLGK
                     RVRTSATLRYRNDRIGVAAVDEMLLSEQRMELAGAYSPSDRWTLSLMMPVVHRLVTAV
                     NLAEFDIWAPGDLEFRARAVIWRDRKFAPRHLVSLIGGLEFPTGSVERDAEGTPLPLE
                     FQAGTGSWDPIAGASYGMFHDPWALYVASVGIFPTVGTADTDVGASFRQTLLVQIQPW
                     NFLAFQLGTEFRFDQPSYIGEVRDPNTGGHITYGSLGLIGLPHEKLVLHFTVAVPMIQ
                     RLDGDHREGLAITAGLIYDI"
     gene            complement(2473..3048)
                     /locus_tag="VFG22_15650"
     CDS             complement(2473..3048)
                     /locus_tag="VFG22_15650"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6417732.1"
                     /translation="MTDAHENAAPKPSILLVDDDERLRARMARAFTARGYEAREADGY
                     DAAIAIAELESTEYAVVDLRMPGKSGLEVVRELHRIDPATKVVVLTGYGSIATALEAV
                     RLGATHYLTKPADVDEVIASFDRKVAEDESGLDAKTEGEPQTPSLARVEWEHIQRVLT
                     DCGGNITKAAERLGIHRRSLQRKLSKFPMRR"
     gene            complement(3045..4286)
                     /locus_tag="VFG22_15655"
     CDS             complement(3045..4286)
                     /locus_tag="VFG22_15655"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6417733.1"
                     /translation="MSAPERASASGAEWLARMRWGALLIQASAILIASAALGVALPLV
                     PMCALVGLGALSNLWLASRKDEAGHLGAFLVVDVILLTGLLYLSGGPTNPFSIIYVVY
                     LAMSAVMLTPRWTWAIAALSIVSFATLFLVHVPSEHLGHAGHEGGFQTHLYGMFIALA
                     LAVALITYFVSQLSLELRRRERALAEAEERTHRWGKLASIATLAAGAAHELGTPLGTI
                     AVAAKEMERQARHVPGAEALVEDAELIREELERCRLVLDRMAAAGGEHVGEVSSLFPV
                     ESLFFEVRARLAPEQARRWVTSTAIDEIEAPKDALVQMVENIARNGFDACESGRVSLD
                     VRAVPGGVELRFVDEGHGMDERAAKRATEPFFTTKNTVDRMGLGLFLARTLVDSLEGR
                     LAIESATGEGTSIVIVLPQDR"
     gene            complement(4283..6130)
                     /gene="dnaK"
                     /locus_tag="VFG22_15660"
     CDS             complement(4283..6130)
                     /gene="dnaK"
                     /locus_tag="VFG22_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HET6417734.1"
                     /translation="MGKIIGIDLGTTNSCVAFVDNGTPQVIPNAEGSRTTPSVVSFAR
                     DGERRVGSVAKRQGVMNPENTVFAIKRLMGLAFDAEPAQKHARLVPYKVVESERGDAW
                     IQIDDHQYSPPEISALVLEKMREVAEAYLGEKVDEAIVTVPAYFNDAQRSATIAAGKI
                     AGLEVRRIINEPTAAALAYGFEEKEGKRVAVYDLGGGTFDISILEIANGVFSVKSTAG
                     DTHLGGEDLDNELVEELARRFEASCGIDVRKDRVALQRLKEQAEKAKHELSSSFETEI
                     NLPFIASDDSGPKHLETKLKRSELEILVSEIIDRTLGPCKQALDDAGIKKEDIDEILL
                     VGGQTRMPMVQSKVTDFFGKKPHKGVNPDEVVAMGAALQGAALTGEVEEVLLLDVTPL
                     SLGVETGGGVFHKLIPRNTTVPTRAKEVFTTSVDNQSFVPIHVMQGEREMAEDNKSLA
                     KFELAPILPAPRGVPEIEVTFDIDADGVVNVSAKDLGTGREQNIRVVASSGLTEKDIE
                     CIIGEADAYKTTDSKRRELAELRNAAEALLYTSERAVAECRELVPEDVIAQVESDIET
                     LHHCIHAGDAIAIRDALETLELSAYKIAEAMYGNVDLDAPAEGEAQSDS"
     gene            complement(6211..6792)
                     /locus_tag="VFG22_15665"
     CDS             complement(6211..6792)
                     /locus_tag="VFG22_15665"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HET6417735.1"
                     /translation="MSEDEGEKPTDEGAEGADEDLIFPEEEAADEPTDLEKAHAERDR
                     MRDQLLRVAADFDNFRKRSRKEIEEVRRRTIEDTLREVLPIVDNLERAADAMSTATEV
                     SAVTDGVHMVLRGFEDIAGRLGLKRVATVGHQFDPTCHDAVQQEETNEHAPGTIVSEV
                     VPGYYLGERLLRPAMVVVAKPPAKTEDPVVGEA"
     gene            complement(6812..7870)
                     /gene="hrcA"
                     /locus_tag="VFG22_15670"
     CDS             complement(6812..7870)
                     /gene="hrcA"
                     /locus_tag="VFG22_15670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HET6417736.1"
                     /translation="MTIVLDERHRRILFAVVTEYIASGQAVSSRRIAKRYAINLSPAT
                     IRNVLADLEDAEFLRQPHTSAGRVPTDAGFRCFVDAIANAGQISRAHRTALIRRLQDL
                     EPGADAVRETGQLLSRLTGTATIMVRPSAAETTLDQIRFMRLSGGRTLAVLVARSGAV
                     ENRVVIMEIEVDDQALERVNNYLAELVGGRTLRDVRDVLAVRLATEEGQYQLLREQAQ
                     QMVQATVEGQNEPAQIFIEGQGALFDRPEFGDAEKIRRYLNAFEEKQKLLVMLEQTLR
                     TDGVQVFIGSETDLDDVDDISVISSPYRRDGAAIGSFGIVGPTRMDYKKYVPLVGFAA
                     DVLSDVLDGRLPGKSKDS"
     gene            7965..8249
                     /locus_tag="VFG22_15675"
     CDS             7965..8249
                     /locus_tag="VFG22_15675"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HET6417737.1"
                     /translation="MRPSAALLPFALMVMAIMTVPTLVLDEQGLPRYQHLQEELHELR
                     RSNEELVKEITTLKGEIDALRSDPSYVERIARDELGMVRGEEFVFQFPPP"
     gene            8333..10420
                     /locus_tag="VFG22_15680"
     CDS             8333..10420
                     /locus_tag="VFG22_15680"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET6417738.1"
                     /translation="MTALVQAALRVRRTARSAWGLAAALLLGAFLIAGFFVTSRAALG
                     WGQVAWLVCWVGLFAHRALQVRRHGSDARSTFELSVLLLVGVHALVQHRGGVASEVYP
                     LTYVAVALVTSFATGSVAIGLLVFALVLGFAIAFLGERIADPALLGLNALFTVCFGVL
                     SYVFTRAEIARVRRKSELELAAQKEKVRDDTRLFRLVAPSSDGVRDEDRLYQTSVQEV
                     RQALYHALQLLHQTLELHTCVLLMPTENEEELRIAELVTKSEDIADGPFALGAGAVGA
                     AVRRQLTTNLQQVRPGYLGICYYRGPSAVRSFIAVPVLERGNLRAVLCADRVVDRAFS
                     PEEEELLRSSTSHVLRAFENERVFMQLERAKREQEILFRVSQSLSTALTQDAVMEVGL
                     SAAKEIVAHDFAAITEYQVEGRRHLVRRATGERALDFQGLRFRDNTSLTAMVVKNRHY
                     LPYRGGFDAKQQVLFTKKAKLKGMDSLLVLPLVVREEPIGTLIVAAKAPGAFGTSVRA
                     TLQALSNQLAVAMANARAVRQLEEMATTDGLTGCLNKRAFLDQIEQKLMAAERFGRKL
                     SVIVTDLDHFKAVNDTYGHAAGDRVLQALGQVLKRVKRETDLVARFGGEEFCVLCEET
                     DARGAELLAERVREELENTELLTELGPLRVTASLGVATFPDHASNAADLFIQGDKALY
                     EAKNSGRNRVCTV"
     gene            complement(10445..10930)
                     /locus_tag="VFG22_15685"
     CDS             complement(10445..10930)
                     /locus_tag="VFG22_15685"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET6417739.1"
                     /translation="MEVLLVRHGEAVDMRSAQTDHDRWLTDAGRQTVTEVGRLLTRLD
                     LRYSCVYTSPLVRAVQTAEILAATQPGFHGPLEVHPALASEAGSTAEALEPLDHADDE
                     DRIVMVTHMPKIGVLAARLSQLARFPSFETAAVCLVRIEDGRGRFQWMLDPETLELRR
                     A"
BASE COUNT         1937 a         3455 c         3402 g         2231 t
ORIGIN      
        1 tccgaggttc tcgaagaggg cagcgcaatc gccgtcggtc ggcccggcca tgcaaccaac
       61 cggggtttcc ggctggttca cgtcgaggga ggcgcctgcg acaagttgag cgaagtcggc
      121 gacgaccgtg tcgctctctg gatcgaacgg atcgagctcg acatcgacgc ggttcggcgc
      181 cgcgcacgtc gtcgttccac ccgcaaaggg atcgccgtcg caacccgtgc tgccgaggtg
      241 catgcgccag gtggtctcgc tggagtcccc cgtgtcgatc cgaaggaact tatagccacc
      301 ctgccagttc cactccatcg acgtgaggtt gagcggcggt tccgctgtgg ccgggtttgc
      361 gtgattgagc tcgaagggca cgcccatctt gaagcgcagg ccgtcgtagc tgccttcggg
      421 aacggttccc gtcactacgc ggttcgttcc cgcgtcaccg aagttggtgc aaccctcttc
      481 gaagtccaga agcgcgacgt tgtcgacctg ccagatgttg ttgtccgtat cgagctcgac
      541 cgcgacgtag tccccgtcgg cgttcctgag ctcgatgtcc tggacgtaga atcggaagtc
      601 gctcacttcg atcgagctgt cgtctgcgcc gagattgtca tacgtggttc cgcatacgaa
      661 cacttcgctt cctaccatcg cctcgaaggc gatcgccacg gactgggagg cgtctcctcc
      721 gctgctgctg tcacaaccaa agcccgccga agcaagcgct gccaccaaga tcatgctttt
      781 cttcatctgc tatttccctg cggtctctag aggtcggcac cccaattcga aagtgctgcc
      841 ttcaacgacg accgtacgtt ttcggttatc tgttggtgca ccgccactga gtcttgcggt
      901 gcaaaatcaa tgccatcgaa ccatgtccgc tggtcaatct gaatggagac gctttccaaa
      961 accgggccct cgaagaccag gggccgatcg aagagcatct cacattcgag aatctccgaa
     1021 attcgcgcga gcaaaggggt ctcctcgccc gtggactcgt cctcgacccg tcccgagatc
     1081 aggacgctat gatgcatcat gtccagggtt tccgcggtta aaccttctgt gtcctggtcg
     1141 gcgggcatcc cgagaacccg gatgccgcag tagcggccgg gcggcggccg aagcgttcca
     1201 gcgaatagcg gcacacccgc gctctccatc aggtcgatga tcagcggcac gccgaggctc
     1261 gttggtgtgc tgagctcgtg ggctctggcc ctcgccggta cgatcagatt ccagagatcc
     1321 gcgacataat tgtcacatcg gatcagctcg actttgccga cggcgatcag ggcgcgctcg
     1381 aggcgcacgg cgtatcccat gtcggttcgg atcgctgcgg tcgttgccgc gggctggtaa
     1441 gcgacatcga cctcgatccc gtcggtcaca tccatgcact gaggaagcgc tgccgcgctc
     1501 agcagcacga tgaccgtgag tagtctagat gtcatagatc agccctgcag tgatcgcgag
     1561 accttccctg tggtccccgt ccagacgttg aatcatcgga actgcgactg tgaaatgcag
     1621 cacgagcttc tcgtgcggca atccaatcag gcccaagctg ccgtaggtga tgtgtccgcc
     1681 ggtgttggga tcgcggactt cgccgatgta gctcggttga tcgaatctga actcggtgcc
     1741 cagctgaaac gccaagaagt tccacggctg gatttgcacg agcagcgttt gtcgaaagct
     1801 cgcgccaaca tccgtgtccg ccgtgcctac ggtaggaaag atcccgaccg acgcgacgta
     1861 gagcgcccaa ggatcgtgga acatgccgta cgaagcgcct gcgatggggt cccaagagcc
     1921 ggtgcccgcc tggaactcca agggtagcgg tgtgccttcc gcatctcgtt cgaccgagcc
     1981 cgttggaaac tcgagacccc cgatcaggct gaccaagtgt cgcggcgcga attttcgatc
     2041 gcgccagatc acggctcgcg ctcgaaattc cagatctcct ggggcccaga tgtcgaactc
     2101 cgcgagattg acggcggtca ccaggcgatg gactaccggc atcatcagcg aaagggtcca
     2161 tcgatccgat ggcgagtacg cgcctgcgag ctccatgcgc tgctcagata ggagcatctc
     2221 atcgaccgcg gccacgccga tgcgatcgtt ccggtagcgc agagttgccg aggtgcggac
     2281 tcgcttgccc aacggctgct ccattcccac gaccgtgagg gtcgggtcgc caaccgcaca
     2341 ggttccgcag gcatatgcct ttgcagcgaa ccacaccgac gcggctagtg ccgcgatcac
     2401 ccaaccaccc ttcactgccg gagcttagat acggacggct ccgatttgta cgtggcaaat
     2461 cgtcgcagcc gctcagcgcc gcatcgggaa tttcgagagt tttcgctgca gtgagcgacg
     2521 gtgaataccg agtctttccg cggctttggt gatattcccg ccacagtctg tcagtacgcg
     2581 ttggatgtgt tcccattcga cccgagccag cgatggagtc tgcggttctc cctcggtttt
     2641 cgcgtcgagc ccggattcgt cttccgcgac ctttcgatcg aaggaggcga tgacctcgtc
     2701 gacgtccgct ggcttggtga gatagtgggt cgcgcccaag cgaaccgctt cgagagcggt
     2761 tgcgatgctt ccgtatccag tcagcaccac caccttggtc gccgggtcga tccggtgcag
     2821 ctcccgaacg acctccaatc cagacttgcc tggcattcgg aggtctacga ctgcgtattc
     2881 ggtgctctcg agctcggcga tcgcaattgc ggcgtcgtag ccatctgctt cccgggcttc
     2941 gtaaccgcgg gccgtgaatg cacgtgccat gcgagcgcgc agccgctcgt catcgtccac
     3001 gagcaggatc gatggctttg gtgcggcgtt ttcgtgtgcg tcggtcatcg gtcttgagga
     3061 agtacaatca cgatcgaggt cccttcaccg gtcgccgact cgatcgccag ccggccctcg
     3121 agcgagtcta cgagcgtccg cgcgaggaac aagcccagac ccatgcgatc gaccgtgttt
     3181 ttcgtcgtga agaacggctc ggtcgcgcgc tttgcggcac gctcgtccat cccatggcct
     3241 tcatcgacga agcgaagctc cactccgccc ggtacggcac ggacgtctag cgacacgcgg
     3301 cccgattcgc aggcatcgaa accattgcgt gcgatgttct ccaccatctg cacgagggcg
     3361 tccttgggcg cctcgatctc gtcgatcgcc gtgctggtga cccagcgcct ggcctgctct
     3421 ggcgccaatc gggcgcgcac ctcgaagaat agggactcga ccgggaagag gctcgaaacc
     3481 tctcccacgt gctcgccgcc cgctgccgcc atccgatcga gcacaagacg gcatcgctcg
     3541 agctcttcgc gaatgagctc agcatcttct accaatgcct ccgcaccggg tacgtgtctc
     3601 gcttgacgtt ccatttcctt ggcggcgacg gcgatcgttc cgagcggcgt tccgagctcg
     3661 tgggcggcgc cggccgccag cgtcgcgatc gaagcgagct tgccccatcg gtgcgtgcgc
     3721 tcttctgctt cggctagcgc gcgttcgcgg cggcgtagct cgagcgagag ctggctcacg
     3781 aaataggtga tcagagccac cgccaaggcc agcgcgatga acatcccgta gagatgcgtc
     3841 tgaaaaccac cttcgtgtcc cgcatgaccc aggtgctcgc tagggacgtg aacgaggaac
     3901 aaggtcgcga acgagacgat cgacagcgcg gcgatcgccc atgtccacct gggcgtgagc
     3961 atgaccgcgg acatcgcgag atacaccacg tagatgatgc tgaaggggtt cgtcggacca
     4021 cccgaaagat agagcaagcc agtcagcagg atgacgtcca cgaccaagaa tgcgcccaga
     4081 tgaccggctt cgtccttgcg cgaagccagc cacaggttcg atagggcgcc aagcccgacg
     4141 agcgcgcaca tcggcacaag cggaagcgcc actcccaagg cggcgctcgc gatgaggatc
     4201 gccgatgctt ggatcagcaa cgctccccag cgcattcgtg ccagccactc cgcaccgctg
     4261 gcagaggccc gttctggcgc gctcacgagt ctgactgagc ttcgccctcg gccggcgcgt
     4321 cgaggtcgac gttgccgtac atcgcctcgg cgattttgta ggcgctcagc tcgagcgtct
     4381 ccagcgcgtc gcggatcgcg attgcatccc ccgcatgtat gcagtggtgc aaggtctcga
     4441 tgtctgactc gacttgggcg atcacgtctt cgggaacgag ctcgcgacac tcagcgaccg
     4501 cgcgttcgct ggtatacagc agggcctccg cagcgttgcg cagctccgcg agctcgcgcc
     4561 gcttcgagtc cgtcgtcttg tatgcgtcgg cttcgccgat gatgcactcg atgtctttct
     4621 ccgtgagccc gctcgaagcg acgacacgaa tgttttgctc gcgaccggtt ccgagatcct
     4681 tcgcgctcac gttgacgaca ccgtccgcgt cgatgtcgaa cgtcacttcg atctctggga
     4741 ccccgcgtgg tgcagggagg atcggggcaa gctcgaattt ggccagcgac ttgttgtcct
     4801 ccgccatttc gcgctcgcct tgcatcacgt gaatgggaac gaagctctgg ttgtcgacgc
     4861 tcgtggtgaa gacctctttc gccctcgtcg ggacggtggt gttccgcgga atcagcttgt
     4921 ggaacacgcc accaccagtt tctacgccca ggctcagtgg cgtgacgtcc agaaggagca
     4981 cctcctcgac ttcgccggtg agcgccgcac cctgaagcgc tgcgcccatg gccacgacct
     5041 cgtcgggatt gacgccctta tgaggctttt tcccaaagaa gtcggtcacc ttggactgaa
     5101 ccatcggcat ccgggtttgg cccccgacga gcagaatctc gtcgatgtct tccttcttga
     5161 tgccggcgtc gtcgagcgct tgcttacagg gtcccagcgt tcggtcgatg atctcggaga
     5221 ccagaatctc gagctcgctc cgcttcagct tggtctcgag atgctttgga cccgagtcgt
     5281 ccgatgcgat gaacggcagg ttgatctcgg tttcgaacga tgaggagagc tcgtgcttgg
     5341 ccttctcggc ctgctctttg aggcgctgaa gtgcaacgcg atccttccgc acgtcgatac
     5401 cgcagctggc ttcaaagcga cgcgcgagct cttcgacgag ctcgttgtcc aagtcctcgc
     5461 ccccgaggtg cgtgtcaccg gcagtcgatt tgacgctgaa gacgccgttt gcgatctcca
     5521 ggatcgaaat gtcgaacgtt cctccgccca aatcgtagac cgcgacgcgc tttccctctt
     5581 tttcttcgaa tccatacgcg agtgcagctg ccgtgggctc gttgatgatg cgccggactt
     5641 cgaggcctgc gatcttgccg gccgcgatgg tggccgagcg ttgggcgtcg ttgaagtacg
     5701 cgggcaccgt cacaatcgct tcgtcgacct tctctccgag atacgcctca gcgacctcgc
     5761 gcatcttttc gagcaccagc gccgagatct caggcggcga gtattggtgg tcgtcgattt
     5821 ggatccaagc atcgccccgc tcgctctcga cgaccttgta tggaaccaaa cgtgcgtgct
     5881 tctgcgcggg ctccgcgtcg aaagcaagcc ccatgaggcg cttgatcgcg aagacggtat
     5941 tttcagggtt catgacgcct tgccgtttgg cgacgctccc tacacggcgc tcgccgtccc
     6001 ttgcgaacga gaccacggac ggtgtcgtgc ggctgccttc tgcgttcgga atgacctgtg
     6061 gtgtcccgtt gtcgacgaat gcgacacagg aattggtcgt accgagatcg atcccgatga
     6121 tcttccccat cttgccctag ccttctttat accccgagcg cgcgggttgt cgcatctaag
     6181 gggtgacgat tgggcggggt agcggccatc tcaggcctcg ccgacgaccg gatcttcggt
     6241 ctttgcgggc ggctttgcca cgaccaccat ggcgggccgg agcaggcgtt cgccgaggta
     6301 gtacccaggc accacctcgg agacgatggt accaggtgca tgctcgttgg tttcctcctg
     6361 ctgcaccgcg tcgtgacaag tagggtcaaa ttgatgaccg acggtcgcga ctcgcttcag
     6421 cccgaggcgg ccggcgatgt cttcgaaccc ccgaagcacc atatggacac catccgtgac
     6481 ggccgaaacc tcggttgcgg tgctcatcgc gtcggccgcg cgctcgagat tgtcgacgat
     6541 tggaaggact tcgcgcagcg tgtcttcgat cgtccgccgc cggacttctt cgatttcctt
     6601 ccgagaacgc ttccgaaaat tgtcgaaatc ggcagcgacc cgcaacagtt ggtcacgcat
     6661 ccggtcgcgc tcggcgtggg ccttctcgag atccgtcggc tcgtcggctg cctcttcctc
     6721 cgggaagatg aggtcctcgt ccgcgccttc agctccctca tccgtcggct tttccccctc
     6781 gtcttcactc atttgatcac gtctgctttc ctcacgagtc tttcgatttg cccgggagac
     6841 gtccgtcgag gacgtcgctt agcacatccg ccgcaaaccc aaccaagggt acgtacttct
     6901 tgtagtccat gcgagtcggc ccgacgatcc caaaggaccc gatcgccgca ccgtcccggc
     6961 gatatgggct cgaaatcacg ctgatgtcgt cgacatcgtc gaggtcggtc tccgagccga
     7021 tgaagacctg aaccccgtcc gtgcgcaaag tctgctcgag catcaccaga agcttctgct
     7081 tctcttcaaa ggcgttgaga tatcgccgga tcttctcggc atcgccgaac tcgggccgat
     7141 cgaagagggc gccctgccct tcgatgaaga tctgggcagg ctcgttttga ccttctaccg
     7201 tggcctgcac catctgctgg gcttgctctc ggagtaattg gtactggcct tcctcggtcg
     7261 cgagccggac tgcgagcaca tcacggacgt cgcgaagcgt gcggccgcca acgagctcgg
     7321 cgaggtagtt gttcacccgc tcgagggcct ggtcgtccac ttcgatctcc atgatgacca
     7381 ccctgttctc gacggcgccc gagcgcgcga cgaggacggc gagtgttcga ccaccggata
     7441 gccgcatgaa tcgaatttga tcgagtgtcg tctcggcggc agagggccgc accatgatgg
     7501 tcgccgtgcc ggtcagcctg ctgagaagct ggcccgtctc acgcaccgcg tctgcgcccg
     7561 gttccagatc ctgaagacgc cgaatcaggg cggtacggtg cgcgcgcgag atctgtccag
     7621 cattggcgat ggcatcgacg aagcaacgaa accccgcatc cgtagggact ctgccagcgc
     7681 tcgtgtgcgg ctggcgaaga aattccgcgt cctccagatc ggccagcacg tttcggatcg
     7741 ttgccggaga cagattgatc gcatagcgct ttgcgatccg tcgtgagctc accgcctgac
     7801 ccgaggcgat gtactcggtc acgaccgcaa agagaattcg ccgatggcgc tcatcgagga
     7861 ctatcgtcac cttctcagga aagtagaagc tcggtagctt ctgtcaagcc tgcgaaagac
     7921 ggcaaaaatt tcgcttcggc ccgggtttgc ggcaggctcg cctcgtgaga ccaagcgccg
     7981 cgctgcttcc attcgccctg atggtgatgg cgatcatgac ggtcccgacg ctggttctag
     8041 acgagcaggg tctaccccgg taccagcact tgcaggagga gctgcacgag cttcgccgct
     8101 cgaacgagga gttggtaaag gaaatcacca cgctcaaagg ggagatcgac gctcttcgta
     8161 gcgacccgag ctatgtcgag cgtatcgcaa gggatgagct cggcatggtc cgtggcgagg
     8221 aattcgtctt ccagttcccg ccgccctgat ggcgctggta ggtgggcaga ttgcccacat
     8281 ttcggcgctt ttcttgctga tcgaaggaga gcgagcgtaa caacccgttg gtatgacggc
     8341 cctcgtccag gcagcgcttc gggtaaggcg cacagcacgg tcggcatggg ggttggcggc
     8401 ggcattgctc ctcggtgcgt tcctgatcgc cggcttcttc gttacgagtc gagcggcctt
     8461 gggttggggc caggtcgcgt ggctcgtttg ttgggtcggg ctcttcgcgc accgagcgct
     8521 tcaggtgcgg cggcatggga gcgacgccag gtcgactttc gagctgagcg tcttgttgct
     8581 cgtgggtgtt cacgcgctgg ttcagcatcg tgggggcgtt gctagcgaag tctaccctct
     8641 cacctacgtc gcggtggcgc tggtgacatc gttcgccacg ggaagcgtag caattggatt
     8701 gctggtgttt gcgctggtgt tgggtttcgc catcgcgttt ctcggcgagc gaattgccga
     8761 ccctgcccta ctcggattga acgcgctatt caccgtctgc tttggcgtgc tgagctacgt
     8821 cttcactcgg gcggagatcg cgcgcgtccg gcggaagtcg gagctcgagc tcgcggccca
     8881 gaaggaaaag gttcgcgacg atacccggct ctttcggctg gtcgcgcctt cgtccgatgg
     8941 cgtgcgcgat gaggaccgtc tctatcagac cagtgtgcag gaggtccgcc aagcgctcta
     9001 ccacgctctg cagctgctcc atcaaacgct agagctgcac acctgtgtgc tcctcatgcc
     9061 cacggagaac gaggaggagc ttcggatcgc ggagctcgtc accaagagcg aagacatcgc
     9121 cgatggaccg ttcgccctcg gcgccggggc cgtgggagcg gccgtgcgtc gacaactcac
     9181 gaccaacctt cagcaggttc gacccggtta cctcggtatt tgctactatc gcggtccgtc
     9241 ggctgtgcgc tcgttcatcg cggtgcccgt actcgaacgg gggaatctaa gggctgtgct
     9301 ttgcgcggat cgggtcgtcg atcgcgcgtt tagcccggag gaagaggagc tccttcgcag
     9361 cagcacgtcg cacgtcttgc gcgctttcga gaacgaacgg gtgttcatgc agctcgagcg
     9421 ggcaaagcgc gagcaagaga tcctctttcg ggtctctcag tctctcagca ccgcattgac
     9481 ccaggacgcc gtgatggagg tcggtctgag tgcagcgaag gaaatcgttg cgcatgactt
     9541 tgcggccatc accgagtatc aggtcgaggg tcggcggcat ctcgtccgtc gtgcaactgg
     9601 cgagcgggcg ctcgactttc aaggtctccg gttccgcgac aacacctcgc tgacggcgat
     9661 ggtcgtgaag aaccgacact atttgccgta ccgcgggggc ttcgatgcga agcagcaggt
     9721 tctcttcacg aagaaagcga agttgaaggg aatggactcg ctcctggtct tgcctctggt
     9781 cgtgcgtgag gagccgatcg gcacactgat cgttgctgcg aaggccccgg gtgcgtttgg
     9841 aacctcggtg cgggcaacgc tccaggccct atccaatcag cttgcggtgg cgatggccaa
     9901 cgcgcgtgcg gttcgacagc tcgaggagat ggccacgacc gacgggctca ccggctgcct
     9961 caacaagcgg gcgttcctcg accagatcga gcagaagctc atggcggcgg agcggtttgg
    10021 tagaaaactc tcggtgattg tgaccgacct cgatcatttc aaggcggtca acgataccta
    10081 cggtcacgct gcaggtgatc gagttctgca agcgcttggg caggtgctca agcgcgtcaa
    10141 gcgcgaaacc gatttggtgg cccgctttgg tggagaagag ttctgcgtgc tctgcgagga
    10201 gaccgatgcg cgtggcgccg agctcctcgc cgagcgcgtt cgggaggagc tcgagaacac
    10261 cgagttgctg accgagctag gcccgctgag ggtcactgcc tcgctcggtg ttgccacgtt
    10321 tccagatcac gcgtcgaacg cggccgatct cttcatccaa ggcgacaagg ccctctacga
    10381 agcgaagaac agcggtagaa accgggtgtg cacggtttga cgggtgctga gcgcccgtcg
    10441 catctcaggc gcgcctgagc tccagcgttt cggggtcgag catccactga aagcggccgc
    10501 gaccgtcttc gatgcgcacc aggcagaccg cggccgtctc gaacgagggg aacctcgcga
    10561 gttggctgag gcgggcagcg aggacgccga tcttgggcat gtgggtcacc atcacgattc
    10621 gatcttcgtc atcggcgtgg tcgagcggct ctagggcctc agccgtgctt cccgcttcgc
    10681 ttgcgagagc gggatgcact tcgaggggcc cgtgaaaacc cggctgggtc gcggcgagga
    10741 tttccgcggt ctgtacggcg cgcaccagcg ggctcgtgta gacgcaggag tatcgaagat
    10801 ccaatcgggt gagcagcctg cccacctcgg tcaccgtctg acggccagcg tccgtcagcc
    10861 agcgatcatg gtcggtctgc gccgaacgca tatcgacggc ctctccatgc cggacgagta
    10921 ggacctccat gcccgaagct tggcatgccg gctagcgcgc gtcccgtcga tgctgcgcgg
    10981 aaagtgcgaa agattcttcg ggagccaaga caagccttcg acggg
//
LOCUS       DASRZF010000354        27751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3703777, whole genome shotgun sequence.
ACCESSION   DASRZF010000354 DASRZF010000000
VERSION     DASRZF010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 27751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27751
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3703777"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /locus_tag="VFG22_15690"
     CDS             complement(<1..955)
                     /locus_tag="VFG22_15690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417740.1"
                     /translation="MRLKLIAGNLAIVLMLGLGSYLVVRTQLRSELSMQLEDRIGDDS
                     ELFARSWRADGARLAEGVSNRAASKAVENVFTASGEDKRRQRAFEAAQHVSKWFQDPA
                     RGRGERPHIVAVIDETGRAIARDTDPNRMVGEPMLAQVTVLRSVLKRGGSRYGVWQHQ
                     DKLLQVGVAAVRNDQGGVVGALLVGYDLSNGFAKRESALVGHDLLFIVADGIYSTSTT
                     VEVRDALQEALYSPPLDVDTKAALGGKPTLSWRADLAGAMYRGITAALPMARGVEAGY
                     VVLAHEGKHNALAEVATMILWLTLLALLGVIIYGLIISNNLM"
     gene            1093..1227
                     /locus_tag="VFG22_15695"
     CDS             1093..1227
                     /locus_tag="VFG22_15695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417741.1"
                     /translation="MFGIDQLAYKGQFDMRSGFEEVGEVAPGGYPSSRGDAEGVEHFL
                     "
     gene            1199..2515
                     /locus_tag="VFG22_15700"
     CDS             1199..2515
                     /locus_tag="VFG22_15700"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HET6417742.1"
                     /translation="MPKVWSIFCDCPELAVDVARDAERIGLSVEPSVLERPWEGAIEA
                     LEAGKLAAMAVEDPLEANILVRLAEAMRAGTTKLPLAIIGPQPLGLPHDLGLPAVHET
                     GPLLAVAGLLELGTERPWAATTKELPSVDRIRLGESVSHRSDGRFVRIDDGLIAHAGD
                     GTKTQPIGTPRDVAAALRAMRASQDPSRPKVPVVQGVEPRTVRDVILGPRRALSDPAS
                     KAALAQYDVPLPIEELCATPSRAASEASRIGFPVRVALASPDLRLWDHPDLAAEVYSA
                     GQARDAFRQIMAMAKNRAADARLLGVSVSASTIARTLLRVRMEPLAAGPVLCEIGFAD
                     PHGRASADTTHTVLPATAKALERVLLRLRGSSLLLAGTPQERTLAVSAIGDVLMRLAA
                     FVHQWRAEVEAVEVNPLAILVGGELELREACVTVGDAFSRSLNASG"
     gene            complement(2546..5308)
                     /gene="uvrA"
                     /locus_tag="VFG22_15705"
     CDS             complement(2546..5308)
                     /gene="uvrA"
                     /locus_tag="VFG22_15705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET6417743.1"
                     /translation="MTADIVIRGAREHNLKNLTVTLPKERLTVVTGPSGSGKSSLVFD
                     TLYAEGQRRYIESLSGHARRVLTKLRRPDVDVIEGLSPAIAVREHAPSRNPRSTVGTL
                     TEVNDYLRVLYSTVGEVHCPVCGERLQAHSVAQVVREALRFPEGERFSVLAPIARAGD
                     AIPKSLVAELRAKGFVRLRVNDAATDLEDVGALPSGVSVDLVIDRLSVRDGIRSRLAE
                     AVELAYQLGDGVVELRLEDGVRHRFSQGYLCFDRHTSIPKISPQLFSFNTPLGACASC
                     DGLGRRRRFTEELAVTDASLSIRRGAIVAWGKPKLAYYRSMMEKIGAAGVDLDAPFAN
                     LPPEQRSIALQGGGGFEGILPGLERRAQDYARRKLAEGGDEERVLEFLDEELGQFART
                     ETCPECMGSRLNEKARSVRITDRAISDVSALDIASLRAWLDTVSVSRQLEEAVRPILR
                     SIEDRLVFLDRVGLGYLSLDRAAATLSAGEAQRVHLATQLGARLSGVLYVLDEPTAGL
                     HPADAQRLLSTLHELRDLGNTLVVVEHDEQTMRAADHLVEMGPGAGEHGGRVMAEGAL
                     DDFVALEQCLTGAFLSGRRRIAVPTSRREGSGSLRVAVGDLHNLHDLRAELPLGSLCC
                     VTGVSGSGKSSLIVDALLPAVRSNVGLSAEAPPGVVVEGGRGLGRVSFVDSAPIGRSG
                     RSNPATYLGLLGPLRDLYAGVPEARARGYQAGRFSFNVKGGRCDACKGEGVQRISMQL
                     LPDAEVECEICGGERYNAETLQIRYRGLSIAEALSLSVDEAYALFEAVPAIRSRLEAL
                     RRVGLGYLALGRRSTTLSGGEAQRIKLARDLAQPARKPTLYIFDEPTRGLHFVDVEQL
                     IKILHELVDEGHSVIAVEHHLDVIRNADYVLELGPGSGPAGGRVVATGTPEELRQTDS
                     ATAKFI"
     gene            complement(5305..6321)
                     /gene="pdxA"
                     /locus_tag="VFG22_15710"
     CDS             complement(5305..6321)
                     /gene="pdxA"
                     /locus_tag="VFG22_15710"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HET6417744.1"
                     /translation="MLAPLAISAGDPAGVGPVVTAAALARCLGTDRALVYGDAAWMER
                     ALQRYGFEALRRIVPADAGLLAPGEVGLVHVADWPLSMVHARAPTIDGGGVQREALEA
                     ACDAAIAGRVRAIVTGPVSKEAVSLSGVSFRGQTEHLARRAGVHVDNVTMMFLGPRLK
                     VALVATHWPIKRVPSTVTRQHIERSVRHLTDALGRWVPDTRPLRIEVSGLNPHAGEGG
                     LLGTEEETIIGPTLEALREEVPYSNGEVELLGPTPAEAAFRYAADGRAHGVVAMFHDQ
                     ATIPSKLLDWGEAVNVTWGLPFVRTSVDHGVAYDAAASGTASESGMIAAIRAAQRLTG
                     GMTE"
     gene            complement(6321..6998)
                     /locus_tag="VFG22_15715"
     CDS             complement(6321..6998)
                     /locus_tag="VFG22_15715"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6417745.1"
                     /translation="MWILVIGREGLEEREGTAVACLHELGCEVKTASLWDRLDTDELA
                     SNPPAVVLIEAGDEADAGRGALVRVRSAAPLQEVPVLIGVTVNALSRLDPSDGFDDFA
                     LMPYVPAELYMRIRGVEWTRSDFAQQERIKMGPLCIDLAAHEVTIDGRPRQLTHQEFA
                     LLSFLCQNRGRVFSRRQLLERVWGVDYYGSSRTVDIHVRRLRMKLGHTVNNLETVRGV
                     GYKMKEP"
     gene            7095..8327
                     /locus_tag="VFG22_15720"
     CDS             7095..8327
                     /locus_tag="VFG22_15720"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET6417746.1"
                     /translation="MATKYWKVEISRLGESEPLFVGAVEAASWPAALQAGRAALGESG
                     GVPAGASCNVNPNGTVTIQDARERRRYQILPTAAPASAPKPPKAAPSTPPPTPAAVRE
                     LGAGAPKPSSPPPATVENKLILLAARDEAPSGSSPIHFRERMYAVPVPLPPGTGEKLA
                     TKLLRSVQEEVQRERGAKFIRIELFDHIWKRAPEHPPVVRVEWKDWKKSIEIEFPLED
                     ETRDSELPRISSVPPPPTEDRLSVAFEACHDLLFLKNRAEALEFAVHLLEDLVPSEAT
                     AAFLLDINTDEFRVVAARGTGARTRQGQALPSSVGLLGAASTLAEHAVLVLADAAADP
                     RYDERVDGVPGLDVRGLLYRPLIHRGRLFGVLQLANGVARRMFSEADCEVVDYVTQQL
                     SAFVARGASLPKAAAVQG"
     gene            complement(8360..8935)
                     /locus_tag="VFG22_15725"
     CDS             complement(8360..8935)
                     /locus_tag="VFG22_15725"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET6417747.1"
                     /translation="MFAKSSISERQAQRFSHRCVRTLASGLGGLVFVVSVASALAPGD
                     TPPAIDMPDQAGKKVDLEELAGKVILIDFWASWCGPCRQEMPVLQAFHEKYAADGLVI
                     IGVNIDSSLKKMNKFLERAPVSFRIVRDPKLAIASRYEPPTMPSSYFISRDGRIRFLH
                     AGFSKEDAPGFEARIKTLLAEPSTRSGAEGR"
     gene            complement(8910..9800)
                     /locus_tag="VFG22_15730"
     CDS             complement(8910..9800)
                     /locus_tag="VFG22_15730"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET6417748.1"
                     /translation="MRSTALCIFMVFASLGCERSTEADSGSAGDAPEIKPAPKASKEP
                     IRSLPQVDISSLDSFSAGIWIDMVNELLSPCGEPVSVARCVSESRKCKRCVQAARYVV
                     RLVDDGYSRDEIRDIYRVRYGDDTKIEKLSSHDSPLRGSPMAPVTLYEFSDFQCPHCK
                     TTAPVLKKIVEESEGKVKLVFKQYPLPMHPKAREAAKAAVAADKQGKFWEMHDLLFEN
                     QDTLQAANFDDFAKQIGLDMKRFKADMAAKATEQKIEADIAEGHAAGVDATPSIYVND
                     RRFVFPPDQLADYVREELDQ"
     gene            complement(9845..10099)
                     /locus_tag="VFG22_15735"
     CDS             complement(9845..10099)
                     /locus_tag="VFG22_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417749.1"
                     /translation="MNELARYLMENAYIDFQGGITIEEVRKFLRDEDSRESRALLSRL
                     IEDKGMDDLMVTIADCLKEYIRTGINEDIVRAQLTSYSES"
     gene            10230..10628
                     /locus_tag="VFG22_15740"
     CDS             10230..10628
                     /locus_tag="VFG22_15740"
                     /inference="COORDINATES: protein motif:HMM:NF025008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HET6417750.1"
                     /translation="MEPRTHLGADPRFCGTLLDLGAGSAQVALQAVSEMAVDTQGLVH
                     GGFIFGLADYAAMLAVNDPHVVLAAAETRFLKPVRLGDRVIANARTVVAKGKQQEVRV
                     EATVNSQKVFEGTFTCIVLERHVLERVGST"
     gene            10628..12376
                     /locus_tag="VFG22_15745"
     CDS             10628..12376
                     /locus_tag="VFG22_15745"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HET6417751.1"
                     /translation="MAEDGGAIIGRVLASRGVKQLFTLCGGHISPILIGAQTNGIKVV
                     DVRDEVSAVFAADAVARMTGVPGVAAVTAGPGVTNTVTAVKNAQLAQSPLVIFGGAAA
                     TLLKGRGALQDIDQISLMEPITKWAVSIKKVNSIQPTVERALDLAQDGVPGPVFVEVP
                     IDVLYPEEMVREMFMKESGVKEGKNLGSKALELYMRGHLYRQFHQPHVSIPPPIRDSL
                     PPKPSSKSHHVDKVASLLRKAERPALVIGSQALVNTRNPESIAGAVTALGVPTWLGGM
                     ARGLLGRHSDIQFRHKRTAALKEADLVIVAGFPFDFRMGYGRSISKRATVVAANLSSS
                     EMRKNRRPEIPVQMHAGEFLRELANTVGGGLGPWSNWFDTLRARERQRDEEIGEQANA
                     GTDLVNPLQLFLRLEEKMADDAVLVVDGGDFVATASYILRPRKPLSWLDPGVFGTLGV
                     GGGFAVGASFVRPGKEVWLIYGDGSSAYSLAEMDTCVRHGLSPIAVIGTDGSWSQIAR
                     EQVPMLGTDVGTVLRRTAYHEVAQGYGAIGLLLDDPSRIDATLDEAKALAASGKPVVI
                     NVHIASTDFRKGSISV"
     gene            12382..14229
                     /locus_tag="VFG22_15750"
     CDS             12382..14229
                     /locus_tag="VFG22_15750"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HET6417752.1"
                     /translation="MATWLQRRKAQSRLRDEVGLWYHPQYKAEALVESARVPGIEVAR
                     GEKILGSLAAEGLIRPKQVRPAPLIPLRELAKAHAESYLEKTARPEYLGRIFGLEAHD
                     IDVDPILIAQRRQVGGTVAAARWAVREPRRVAFNIGGGFHHAEPEAGAGFCVYNDVAV
                     AIRVLRADGFEAPIAIVDLDYHQGNGNIVTFEDDKSVLTYSIHGSIWSHVEAAADQQY
                     QLPSRVDDATYLSKLRDTLPVSLETIHPGLIFYVAGNDVLTGDRLGEFELSRAGVLER
                     DCFVIEMAQKLGCPAVVTLGGGYSDDAWRASSDFIRWLLTGELRVSTETPKSLFEQYA
                     QVAMQLDPQELQGSSGEWILTEADLMGDLTGPQYKSTRMLDYYSRHGIEFALEKYGLA
                     AEVRDRGFTDLKLAVDPTDPERQHLTLHATKNGSEHLLVDLVLRRVTRPAPEGLEPPD
                     EIRLLYIEWMMLQNPTEAFSLRQPQWPGQDHPGLGVGEKAMHMLFQGAQRLGLDGLAH
                     HPSRYHIAFIGGGQSFFLDPELQGRFDAIREVLAPLDLAEAAWKMERGEVCWSDGEPV
                     AWIPEDMVIPASDRFFAYLGSIPYQRPRAESQARAKARGIVLEPTRRTS"
     gene            complement(14210..14503)
                     /locus_tag="VFG22_15755"
     CDS             complement(14210..14503)
                     /locus_tag="VFG22_15755"
                     /inference="COORDINATES: protein motif:HMM:NF015541.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HET6417753.1"
                     /translation="MSSLEILAWVTRLDENSPGNVPTGVPILLIQGQQDRVVPILLTN
                     VLENRLCEIGNEVDYRVFPRYGHNDSTEKTMPLMLEWTAARFAGEPASTTCGE"
     gene            complement(14805..15188)
                     /gene="rnpB"
                     /locus_tag="VFG22_15760"
     ncRNA           complement(14805..15188)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFG22_15760"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(15225..17522)
                     /locus_tag="VFG22_15765"
     CDS             complement(15225..17522)
                     /locus_tag="VFG22_15765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417754.1"
                     /translation="MTAAPAAAQEVACPPGNLLAGKTPVARPGVTHSERLNDGVKAGE
                     GDPWESELTSVLADAESQVIYDLGATTRVTAVDLQGDNNDDYIVELSEDRRNFSPLWV
                     GDPVSGQGMRRRSARGLEGQGRYVRVRGQGGDGYFSLAEIQVFCETPAAWPPPVEVKN
                     TSKWWWNKILEKRDHRYRLALAVLGLLFFVALFRIEESKKALVGASALSVAMLGLAGY
                     RLYGARLAPWFASWGVYVLGILVVAWAARGAWLARQEREPEGRTWWEQGALVWIIVAS
                     AIAWVNFGVFHSSRVVHYWDTFHYYVGSKYFEENEYERLYQCVLAADYEDRGEAELQK
                     RKIRDLVDNRLHFVSKADALAYQSHCAEHFTQERWDAFRQDTRSFRTVMGSAWWKDML
                     MDHGYNASPISNMVAAFLTNIGWEEQVPRISSAQVAPDDLKRFQERILRYTMIDLGLY
                     AGAFLMILWAFGLRSTALAVLVWGTGYPWAYFWTGGSFARVPWLFMAVAAVCLLKRGF
                     PLLSGFALSWSALLRLFPAALAGGPMASLIDRFIRRKKIARPWLDSTDKRFILGGILG
                     LLVLVTASVAINGVDAHSKFLGNTIKHSETPLTNHMGLPTILSYRPSTVGRFTKDSSL
                     DDPWAKWKQARKETKHDRRWLHALILLGMFVLLALAGRRLAGWAVLAASSILIIGFFE
                     LTCYYYSFVILMAPLAIERLRYSVAFILMGITGLILQFFIGWYDEQYIWETVAVLLAL
                     TYILTDLVREPLPESSPASADIQAL"
     gene            17694..18038
                     /locus_tag="VFG22_15770"
     CDS             17694..18038
                     /locus_tag="VFG22_15770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417755.1"
                     /translation="MMGSNSSKKAPAASTETDMVLVYGQSEDGQRYGVIRQRGAEIQA
                     GTMRPLQEGKPILGEVVRLKPREESPALFDVEVQHQGSSARGRPAKVASEDYRRGWES
                     IWSNKRRNQALN"
     gene            complement(18102..18629)
                     /locus_tag="VFG22_15775"
     CDS             complement(18102..18629)
                     /locus_tag="VFG22_15775"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HET6417756.1"
                     /translation="MKDAASAEVVDGDSNPEEERESLADALDEPGTDERRGRRKRRSR
                     ARARTISIRRLSKAELNRGRELFPETDYWKPRSRLECKDMERPCPFVSCKYHLFIDVH
                     PVRGSIKLNFPDIEIWEMTETCALDVADRGGITLEEVGEIMNLTRERVRQVETAGLAR
                     LQEYRDVERLKDYVT"
     gene            complement(18653..19213)
                     /locus_tag="VFG22_15780"
     CDS             complement(18653..19213)
                     /locus_tag="VFG22_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET6417757.1"
                     /translation="MTLPRGQQLRYRESEARTWSDGELEQIERAHAEGMSVQQIVETF
                     TARGSRLSEATFRKYVQLGLLPRSVRVGRKGKHRGSQGLYPATAVRQIDHIRRLMHQG
                     FTMEEIQKEFLFVRGDIDALRRQLKRVYRAIEDAVHEQEREGSDDLGVGDALNDVREL
                     GKELVQKLEAIERRLTMRARMARAAV"
     gene            19432..20286
                     /locus_tag="VFG22_15785"
     CDS             19432..20286
                     /locus_tag="VFG22_15785"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6417758.1"
                     /translation="MDSRAFRRFRRNKGALAGLVLVVAVSVLGLLGPWLAPFDPVEQF
                     TDSLLLYNGLPAGPGVEPGHWLGGDVLARDELSRLLSGARVSMLVAYLATALAVLIGV
                     TLGLISGYVGGRVDTFLMRMVDVVLSMPFLLIAIVMQRVWDAPGIWTICIVLGVLSWT
                     TLARVTRAKTMEARELDYIQAARALGLRHFRILARHVLPNVIGPIIAIATILVARMII
                     AESALSFLGLGVRPPTASWGSMLNDSQSLLLGVPRLTLYPAVLISMAVFGFNLFGEGL
                     RDAFDPKD"
     gene            20264..21994
                     /locus_tag="VFG22_15790"
     CDS             20264..21994
                     /locus_tag="VFG22_15790"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6417759.1"
                     /translation="MRSIPRTERNAPTRWLGGAAAILLAGLLVAWVVAHFPKAPLGPR
                     YIGAGHHTPQSGGTFIFAAGSNIHTLDPHFAYDTLSTAACRLIYDGLLDYDYEGNMIP
                     SLAAELPEISDGGRVFRFKLRDGIKFHNGREITAEDVSWSLHRLLSESVGSPGYPFFK
                     SIVGAQQYHAGEVEQIQGIVVIDEKTIEIQLDEADQTFLNALAMPFAYPIAKEHVEKL
                     EAEEGRDAVGRHPVGAGPFKFSRWERGVQVELERFEDYWAPAARPDRMVFLENISADV
                     ASARFRNGDLDILYRPSKVHRLFLRESEAWAPYLAEAPSASLFALGLNCELPPFDNVH
                     IRRAVAFALDRSKLEKLDPGQVIAATQILPPMLAPYDPELPTKQRFDLARAKEEMRLA
                     GFPDGLSEPITLWVRGESEVRVAQLFQQDLKAIGIEIELKMVSFATYLKETGKPRVAQ
                     AAFTGWHQDFPDPSNFMDILFHSRSIHPENSENRSFYRNPKLDEILDRARPEIDREKR
                     LALYAEANAILADEAPWAFLYYPVDMFAWQPYVKGFRPHPVWLNEYRNIWLDLPRSRV
                     SQAIYSEEAP"
     gene            21991..22992
                     /locus_tag="VFG22_15795"
     CDS             21991..22992
                     /locus_tag="VFG22_15795"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6417760.1"
                     /translation="MIRFIAQRALWGILVVMSVVTLVFFLIFVAGDPALTVLGPQARA
                     EQIADFKAKKGLDRPVPEQFLSYLGVMPCVRKDSSKYERDDGSRGFCGILQGDLGESY
                     SHHESVANVIGYRLPRTLLLASLAVLFELIIGLTAGIIAALRRNRWSDTIIMFATFVG
                     ISLPTYVTGPIALLLFAFLLGWFPFGGYGVDPLDHVYHALLPSVILAIAGAATYARVM
                     RGELIETLRHDHVRTARAKGMPMAQVVIKHAIRNALLPIATLIGLSLPFLVGGAIITE
                     KIFAWPGLGLLTIESIESLDAPVIMAVVLMFGIAVQIGNLLADIAVAALDPRIRLRD"
     gene            complement(22989..23762)
                     /locus_tag="VFG22_15800"
     CDS             complement(22989..23762)
                     /locus_tag="VFG22_15800"
                     /inference="COORDINATES: protein motif:HMM:NF015444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF296 domain-containing protein"
                     /protein_id="HET6417761.1"
                     /translation="MTSSDELLSFETPLSRHILGRVPRGAGLQDAFRKLVDEHRLRTA
                     WVSAMGAFEWIELTEYNQAERRYEAPHRFEGCEILSLQGNLSERDGAPFWHMHVTISL
                     RENGRDVTYGGHVVDGSVFALEFRIDCFDELVLQRANDEATGLQLWTEATAPDSGGGA
                     VSPMAPRSEGVSWAMAAALSARAEPRAALEHKPEQGDWIEHAKFGLCRIEGVSGDGVC
                     FIKLPDSRRKKIKIDAMQVSAPRIDDGRRIFQVSIKPRG"
     gene            complement(23759..24403)
                     /gene="pgsA"
                     /locus_tag="VFG22_15805"
     CDS             complement(23759..24403)
                     /gene="pgsA"
                     /locus_tag="VFG22_15805"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HET6417762.1"
                     /translation="MEEATPKRPSIREDAINLPNLLTMLRIALIPGVLWLLWDGTPKA
                     SFWAAMLYIGTALTDLIDGYLARKWGQISVLGKFLDPLADKLLVMATLISLAYLGRLP
                     FLGVVAVIIMLGRELAVTALRTIAMSEGVVMAAMRTGKDKTALQMVAILMLIVHHRYT
                     IYFGFYQTEFDFAQVGLVLLYLSLALALLSAGEYFKLFVDAVDAKEERLTQAAS"
     gene            24547..25518
                     /locus_tag="VFG22_15810"
     CDS             24547..25518
                     /locus_tag="VFG22_15810"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET6417763.1"
                     /translation="MLELTRNVADRARTTLGIATQNFAVQDAIAVAFHAYLFIRISLA
                     PSSPDAIVAGRLALALLVVTACSMILSRGELIRHQKARALTYRLGLFVPMVLSYFEMR
                     ILLPALHPELMDYRLFAIDLWLLGTTPSVWMQAWNLQPIVEWFSFSYYTHFFVLIIIL
                     IPALIFGKGRRLQELMAGGMLIVALGHFLYTLVPGAGPYATLGFAEPLHGGFWWNQVR
                     ITVATAGAQLDIFPSLHTAWPAYFTLHAYAHRKVQPFRALWPVLGFIALNIIIATMFL
                     RWHWFIDVAMGLVLAVVARRFAVAVATREEERGSRSDPRQPVWERLY"
     gene            complement(25515..25793)
                     /locus_tag="VFG22_15815"
     CDS             complement(25515..25793)
                     /locus_tag="VFG22_15815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417764.1"
                     /translation="MIRLARACVSALLLSVAWAADLAYACPSCASPLEENRQAFVDTT
                     IFLTLVPLMMMGGFIWWLRRRFRAMDEAPEIPVPESLLRDAGRFSKGG"
     gene            complement(25790..26380)
                     /locus_tag="VFG22_15820"
     CDS             complement(25790..26380)
                     /locus_tag="VFG22_15820"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HET6417765.1"
                     /translation="MPDDALGRPYGVPARRAPVLPNGVLGMLIFVATEIMFFSGLISA
                     FVIGKSNAIGGWPPPGQPRLPVEETALNTAALLASGVALYFANRAFQRSSPNTKRLLL
                     LAIGLGGFFVLFQGAEWVALIREGLTLTSGQLGGFFYLLVGTHALHVVAGFIILLYTY
                     RRLSEGNLDRFTFWPAQIFWYFVVGVWPVLYWQVYL"
     gene            complement(26399..26824)
                     /locus_tag="VFG22_15825"
     CDS             complement(26399..26824)
                     /locus_tag="VFG22_15825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HET6417766.1"
                     /translation="MAEHQSHADDHGQAHYVKIWAVLLVLLILSVLGPIVAPHVEFGF
                     LKAWMITLMTAFGIAVVKAYLVAANFMHLNVEKKYISYLLATMLMLMLLFFAGTAPDV
                     MEHKGQNWENVAAETEVEQALESQSEAKTHAGEHHDAQH"
     gene            complement(26837..27415)
                     /locus_tag="VFG22_15830"
     CDS             complement(26837..27415)
                     /locus_tag="VFG22_15830"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HET6417767.1"
                     /translation="MSMTKPIATTRSATGIATGKLAVWWVIGSEIVIFGGLLASYIMH
                     RLGHPEWAEYASHTNTWFGAANTFVLLTSSLFAVLAHHAAEHGDGKKAAKWLLGTCGG
                     GLVFLCIKAVEWTGEIRHGYTITANGFWHFYYTAAGLHALHVIAGCIIMLIIANEARR
                     GKELQRVELIGIYWHFVDIVWIFLFPLLYIAK"
     gene            complement(27426..>27751)
                     /locus_tag="VFG22_15835"
     CDS             complement(27426..>27751)
                     /locus_tag="VFG22_15835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417768.1"
                     /translation="RIYNFQNFPDLAGLQDLRVLATSALLVMLVFQAVFLFNFVTSLI
                     KGKPAGKNPWKANTLEWTAESPPPHGNWPELPNCYRGPYEYSVPGRDEDYWPQDVPPD
                     TAPAE"
BASE COUNT         5252 a         8856 c         8405 g         5238 t
ORIGIN      
        1 ccatcaagtt gttggagatg atcaatccgt agatgatcac gccgagcagc gccagcaggg
       61 tcagccagag gatcatcgtc gccacttccg caagcgcgtt gtgtttgccc tcgtgggcca
      121 aaaccacgta gccagcctcc acgccgcgcg ccatcggcag cgcagcggtg atccctcggt
      181 acatggcgcc tgcgaggtca gcacgccaag aaagggttgg tttaccgcct agcgctgctt
      241 tcgtgtcgac atcgagcggg ggtgaataga gggcttcttg gagcgcatcg cgcacctcga
      301 cggtcgtcga cgtgctgtag atgccgtccg ccacgatgaa cagaaggtcg tgtccgacca
      361 gcgcagactc ccgcttggca aagccgttcg aaaggtcata gccgaccagt agcgccccta
      421 cgactccgcc ttgatcgttt cgaaccgccg ctacaccaac ctgcagcagc ttgtcctgat
      481 gctgccaaac gccatagcgc gagccgccac gtttgagaac actgcgcaga accgtaacct
      541 gagcaagcat gggctcgcca accatgcgat tcggatcggt gtcgcgggcg atggcccggc
      601 cggtctcgtc gatgacagcc acgatgtgcg ggcgctcccc gcgtccgcga gccggatcct
      661 gaaaccactt ggagacatgc tgcgccgcct cgaaggcgcg ctgccgccgc ttatcctcgc
      721 cagacgcggt aaacacgttt tcgacggcct tgctcgcagc acgatttgac accccctcgg
      781 ccagtcgagc cccatcggca cgccaggagc gggcgaaaag ctcggaatcg tcgccgattc
      841 ggtcctcgag ctgcatcgaa agctcgctcc gcagctgggt acgtacgacc aggtacgacc
      901 ccaacccgag catcaatacg atggcgaggt tgcccgcaat cagcttcagg cgcatgtcgc
      961 gactctcctc agaatgaccc ccagccgctg attctccctt ttgatcagag gggttacacc
     1021 agcggagccc ggattatcac cctgcgcttc tgggcgtcaa gctcgtgtca ccctaaacct
     1081 agagctgatc cgatgtttgg gatcgaccag ctcgcgtaca aaggacaatt cgacatgagg
     1141 tccggattcg aggaagtggg cgaagtggca cctggcgggt atccttcgag tcgcggcgat
     1201 gccgaaggtg tggagcattt tctgtgactg tccggagctg gcggtcgacg tggcgcgcga
     1261 cgccgagcgg atcggtttgt ccgtggagcc cagcgtcctc gagcgaccct gggaaggcgc
     1321 gatcgaggca ttggaagcgg gcaagctcgc cgccatggcg gtggaagatc cgctcgaagc
     1381 caacatcctc gtgcggctgg ccgaggcgat gcgcgccgga acgacgaaat tgccgctcgc
     1441 gatcataggc ccgcagccgc tcggcctccc tcacgatcta ggccttcccg cggtccacga
     1501 aaccgggccg cttctggcgg tggccgggct tctcgagctc ggcacggagc gcccctgggc
     1561 tgcgaccacg aaggagctcc cctcggtcga ccgaattcgg ctcggcgaaa gcgtgagcca
     1621 tcggagcgac ggtaggtttg tgcgaatcga cgatggccta atcgcgcatg cgggcgacgg
     1681 gaccaagacc cagccgatcg gtacaccgcg cgacgtggcc gcggcactcc gcgcgatgcg
     1741 cgcatcgcag gacccgtcgc ggccgaaggt tccggtggtc cagggagtcg agccccgcac
     1801 ggtccgcgac gtgattctcg gcccgaggcg cgccctttcg gatcccgcga gcaaggccgc
     1861 cctcgcgcag tacgatgttc ctcttccgat cgaagagctt tgcgcgactc catcacgcgc
     1921 cgcctcggag gcctcccgaa tcggatttcc cgtccgtgtc gcgctggcct cacccgatct
     1981 ccgactctgg gatcacccgg atttggccgc cgaggtctat tccgcgggtc aggctcgcga
     2041 cgcgtttcgc cagatcatgg ccatggcgaa gaaccgtgcg gccgatgcgc gattgcttgg
     2101 agtgagcgtg agcgcatcga ccatcgcgcg cacgcttcta cgcgtccgga tggagccgct
     2161 tgccgctgga cctgtgcttt gcgagatcgg ctttgcggac ccgcatggca gagcctccgc
     2221 ggacacgact cacacggttc ttccagcaac ggcgaaggcc ctcgaacgcg ttctgctgcg
     2281 gcttcggggc agctcacttc tcctcgcagg caccccccag gaacgaaccc tggcggtgag
     2341 cgcgatcggt gacgtcctga tgcgactcgc agccttcgtc caccagtggc gggctgaagt
     2401 cgaggccgtc gaggtcaacc ctcttgcgat tctggtgggg ggcgagctcg agcttcgcga
     2461 agcctgcgta accgtaggag acgccttcag ccgttcgctc aacgcgagcg gttgactggg
     2521 gtcgcccgca ctcggcctcc ccggttcaga tgaacttcgc agttgcagag tcggtttggc
     2581 gcagctcctc cggagtgccc gtggctacga ctcgtccacc tgccgggcct gagcccgggc
     2641 cgagctcgag gacgtaatcg gcgttgcgaa tgacgtccag atggtgctcg acggcgatga
     2701 cactgtgccc ctcgtccacg agctcgtgga ggatcttgat cagttgttcc acgtcgacga
     2761 agtgcaagcc tcgggtcggc tcatcgaaga tatagagagt gggcttgcgc gcaggctggg
     2821 cgaggtcgcg ggcaagcttg atgcgctggg cttcaccgcc gctgagggtg gtcgagcgcc
     2881 ggccgagcgc caaatacccg agaccgacac gacgtagagc ttcgagacgg ctacgaatcg
     2941 cggggaccgc ttcaaacaac gcgtaggcct cgtcgaccga cagagagagc gcttcggcga
     3001 tgctcaggcc acggtatcga atctgcaagg tctccgcgtt gtatcgctcg cccccgcaga
     3061 tctcgcactc gacctccgca tccggaagga gctgcatcga gatgcgctga accccttccc
     3121 ccttgcacgc gtcacatcga ccacccttca cgttgaagct gaaacggccg gcctggtagc
     3181 cccgtgcacg ggcctcggga actcccgcat agaggtcgcg caaaggaccg agcaaaccga
     3241 gataggtcgc cggattcgag cgcccgctcc ttccaatggg tgcggagtcg acgaaggaaa
     3301 cgcggccgag acctcgcccc ccttcgacga cgacccccgg gggcgcctcc gcgctcaaac
     3361 cgacgttgga ccgaaccgct ggcaggagcg cgtccacgat cagcgagctc tttccgcttc
     3421 cactgacgcc tgtgacacag cagagggagc ccaatggaag ctcggcgcgc aggtcgtgca
     3481 agttgtggag atccccgacc gcaacacgga gcgagccgga accctctcga cgactcgttg
     3541 gaaccgcaat cctgcggcgc ccagagagga acgcgcccgt caagcactgc tcgagcgcaa
     3601 cgaaatcgtc gagcgcgccc tcggccatga cccgaccgcc atgctcgccc gcccccggtc
     3661 ccatttcgac tagatggtcc gcggcacgca tcgtctgctc gtcgtgctcg acgacgacca
     3721 aggtgtttcc taggtcgcga agctcatgga gagtgctgag taagcgctga gcgtcggctg
     3781 gatgcagccc ggcggtcggc tcgtcgagaa cgtagagcac ccccgaaagc cgcgccccca
     3841 actgcgttgc gagatgaacg cgctgcgctt cgcccgccga aagggtcgcg gctgcgcgat
     3901 cgaggctcag gtagccgaga cccacgcgat cgaggaacac gagacgatct tcaatcgacc
     3961 gtagtatcgg ccgcaccgct tcttcgagtt gccgggaaac cgaaacggtg tcgagccatg
     4021 ccctcagcga agcgatgtcg agcgcgctca cgtcgctgat cgcgcgatct gtgatccgca
     4081 ccgatcgcgc tttctcgttg agccgcgagc ccatgcattc ggggcaggtc tcggtgcgtg
     4141 caaactgtcc gagctcttcg tcgaggaatt cgagcacgcg ttcttcgtcc ccgccttctg
     4201 caagcttgcg tcttgcgtag tcctgcgcgc gacgctcgag cccgggcaag ataccctcga
     4261 atcctcctcc accttggagc gcgatgctcc gctgctcagg aggaagattc gcgaacggag
     4321 catcgaggtc gacgccagcg gcaccgattt tctccatcat ggatcgatag taggcgagct
     4381 tgggcttgcc ccaagcgacg atcgcgcctc gccgaatcga gagactcgca tcggtcaccg
     4441 ccaactcctc ggtgaaccgt cgtcttcttc ccagtccgtc gcagctcgcg cacgcaccaa
     4501 gcggcgtatt gaatgaaaag agctgcggac tgattttcgg aatcgaggta tgccggtcga
     4561 agcacaggta cccctgtgag aaccggtgac gcacgccgtc ctcgagccgc aactcgacga
     4621 cgccgtcgcc cagttggtaa gcgagctcga ccgcttcggc gagccgggat cggatcccat
     4681 cgcgcaccga caatcggtcg atgaccaggt cgaccgacac gcccgatggc aatgccccaa
     4741 cgtcctccaa atcggtcgcg gcatcattta cgcggaggcg aacgaatccc ttcgcccgga
     4801 gctcggcgac cagcgatttg ggaatcgcgt cgcccgcacg tgcgatgggc gcaaggacag
     4861 aaaagcgctc gccttccgga aagcgcagag cttcgcggac cacctgcgcc acggagtggg
     4921 cctgcaagcg ctcgccacat accgggcaat gcacctctcc gacggtggaa tagagcacgc
     4981 gcaaatagtc attgacctcg gtcagcgtgc ctaccgtgga acgtggattg cgcgaaggtg
     5041 cgtgctctct cacggcaatc gcaggagaca gaccttcgat gacgtcgacg tcgggacgcc
     5101 gcagcttcgt gagaacccgc ctcgcatgcc cactcaacga ctcgatgtat cggcgctgcc
     5161 cttccgcgta gagcgtgtcg aacaccagac tcgacttccc actgccactc ggaccggtga
     5221 ccaccgtgag cctctccttg ggaagggtca cggtcaggtt ctttagattg tgctcgcggg
     5281 ctcctcgaat gacgatatcc gccgtcattc cgtcataccg ccggtgaggc gctgggcggc
     5341 acggatcgcg gcaatcattc cgctctccga cgcggtaccg ctcgctgccg cgtcgtaagc
     5401 gacgccgtgg tcgacactcg tgcgaacgaa cggcagtccc catgtgacgt tcaccgcttc
     5461 accccagtcg aggagcttcg acggaatcgt ggcctggtcg tggaacatgg caacgacccc
     5521 atgcgcgcga ccatcggcgg cgtagcggaa cgcggcctcg gcgggcgtcg gcccgaggag
     5581 ctcgacctca ccgttcgagt acggcacctc ttcgcggagc gcctcgagtg tggggccgat
     5641 gatggtttct tcctcggtcc cgaggagtcc gccctctccg gcgtgcgggt tgaggccgga
     5701 aacctcgatg cgaagcggtc tcgtatccgg cacccatcgt ccgagcgcgt cagtcaagtg
     5761 acgaacgctg cgctcgatgt gttggcgagt cacggtggac ggcacacgct tgatgggcca
     5821 gtgcgtggcg acgagggcga ccttcaatcg cgggcccagg aacatcatgg tcacgttatc
     5881 gacgtgaacg ccggcgcgcc gcgcgagatg ctcggtctgg ccgcgaaatg agacgccgga
     5941 caggcttacg gcctccttgc tgaccggccc ggtcacgatc gctcgtacac ggcccgcgat
     6001 cgcggcatca cacgcggcct cgagggcctc gcgctggacg cctccgccgt cgatcgtggg
     6061 ggctcgcgcg tgcaccatgc tcagcggcca atccgcaacg tgaaccaggc cgacttcgcc
     6121 tggagctaag agtcccgcat ctgcgggaac gattcgacgc aaggcttcga agccatacct
     6181 ctggagcgcc cgctccatcc aggcggcatc gccatagacg agcgctcggt ccgttcccaa
     6241 acaccgagcc agcgccgccg ccgtcaccac agggccgaca cctgccgggt cgcctgcgga
     6301 aatcgcgagc ggggcaagca tcacggctcc ttcattttat agccaacacc ccgtacggtc
     6361 tcgaggttgt ttaccgtgtg cccgagcttc atgcgcaggc gccgcacgtg aatgtcgaca
     6421 gtacggctgc tgccatagta gtccacgccc cagacccgct cgagaagctg ccgccgcgaa
     6481 aagacgcggc cgcggttctg gcaaaggaag ctgaggagcg cgaactcctg atgggtgagc
     6541 tgccgtggac gcccgtcgat cgtgacttca tgcgcggcaa gatcgatgca gagcggaccc
     6601 atcttgatgc gctcctgctg agcgaagtca ctccgggtcc attcgacgcc ccgaattcgc
     6661 atgtacagct cggcgggcac gtagggcatg agagcgaagt cgtcgaaacc atcgctaggg
     6721 tcgagtcgcg aaagcgcatt gacggtcacg ccgatgagga ccgggacctc ctgaagcggc
     6781 gcggccgaac gaactctgac cagggccccg cgaccggcat cggcctcatc gccggcctcg
     6841 atcaacacca ccgccggggg gttgctcgcc agctcgtccg tatccaagcg atcccagagg
     6901 gaagccgtct tcacctcgca gccaagctcg tgaagacagg ccacggccgt tccttcacgt
     6961 tcctcgagcc cctcacgacc gatgactagg atccacaccc gcggttccta caacggcgac
     7021 caagagcatg ccacccggga tgcgacttcc taggaaatct taggtatcat gcttgggcgc
     7081 aagatcgggt tcaggtggct accaagtact ggaaagtaga aatcagtcgg ctcggcgaat
     7141 ccgagccgct cttcgtcggc gccgtcgaag cggcgagctg gccggccgct ttgcaagctg
     7201 gccgtgccgc actcggcgag tccggtggtg tgcccgcggg cgcgagctgc aacgtgaacc
     7261 cgaacggaac cgttacgatt caggatgcac gagagcgccg gaggtatcag atcctcccaa
     7321 ctgcagcccc cgcctcggcg cccaagcctc cgaaagcggc gcctagcacg ccaccaccga
     7381 cccccgcggc cgtgcgagag ctgggggccg gcgcaccaaa accgagcagc cctcctccgg
     7441 cgactgtcga gaacaagctg atcttgctgg ctgcgcggga tgaagcgccc agcggctcga
     7501 gtccgatcca ttttcgtgag cggatgtacg ccgtgcctgt tccgctcccg cccggaaccg
     7561 gagaaaagct cgcgactaaa ttgctgcgca gcgttcagga agaggtccag cgcgagcggg
     7621 gcgcgaagtt catccgaatc gagctcttcg atcacatctg gaagcgagcg cccgagcatc
     7681 cgccggtcgt gcgggtcgaa tggaaggact ggaagaagag catcgagatc gagtttccgc
     7741 tcgaagatga aactcgcgat tccgagttgc cacgaatcag cagcgtcccg ccccccccta
     7801 cggaggatcg gctctcagtc gcgttcgaag cctgccacga cctcttgttt ctcaagaacc
     7861 gcgcggaggc tctcgagttc gcggtccatc ttctagagga tctagttccg tccgaggcga
     7921 cggcagcgtt cctgctggat atcaacaccg atgagttccg cgtcgtcgcg gcccgaggca
     7981 cgggtgcgag aactcggcaa ggacaagccc ttccctcatc ggtaggcctc ctcggcgcag
     8041 ccagcacact ggccgaacac gcggtgctgg tcttggccga cgctgccgcg gacccgcgct
     8101 acgatgagcg agtcgatggg gtgcccggtc tcgacgtgcg tggactcttg tatcgccctt
     8161 tgatccatcg aggccgcctg ttcggagtgc tccagctagc caacggagtg gcgcggcgaa
     8221 tgttcagcga agccgactgc gaagtcgtcg actacgtcac tcagcaactg agcgccttcg
     8281 tcgcgagagg cgcttcgcta ccgaaagcgg cagcggttca gggttgatcg cggattcttc
     8341 caagcccccg agtctcagct catcgcccct cagcgccgct tcgggtcgac ggttcagcaa
     8401 gaagagtttt gatgcgcgct tcgaatccgg gcgcgtcctc cttgctgaat cctgcgtgta
     8461 agaaccgaat tcttccgtcg cgcgagatga aataagacga gggcatcgtc ggaggctcgt
     8521 acctcgacgc gatcgcaagc ttagggtcgc gcacgattcg aaagctgacg ggagcacgct
     8581 caagaaactt gttcatcttc ttgaggctgc tgtcgatgtt gacgccgatg atgacgagcc
     8641 catcggcggc atacttctcg tggaacgcct gcaggaccgg catctcttgt ctgcatgggc
     8701 cgcaccagga cgcccaaaaa tcgatcaaga tgacctttcc tgcgagctcc tccagatcga
     8761 ccttctttcc cgcttgatcg ggcatgtcga tcgccggcgg cgtatcaccg ggcgccagag
     8821 cgctggccac cgagacgacg aaaaccagcc cgccgagtcc cgaggcgagc gttctcacgc
     8881 agcggtgcga aaatcgctgc gcctgccgct cactgatcga gctcttcgcg aacatagtcg
     8941 gcaagctgat caggtggaaa cacgaaccgg cggtcgttca cgtagatcga tggcgtcgcg
     9001 tcgactcccg cggcgtgccc ttctgcgata tccgcctcga tcttctgttc ggtggctttt
     9061 gctgccatgt ccgctttgaa gcgcttcata tcgagcccga tctgcttggc gaagtcgtcg
     9121 aaattggcgg cctggagcgt gtcttgattc tcgaagagca agtcgtgcat ttcccagaac
     9181 ttgccttgct tgtccgccgc gacggcagcc ttggccgcct cgcgcgcctt cggatgcatc
     9241 ggtagcgggt actgcttgaa gaccaatttc accttgccct cggattcctc gacgatcttc
     9301 ttgaggacgg gagcggtcgt cttgcagtgc gggcactgga aatcgctgaa ctcgtagagc
     9361 gtcaccggcg ccatcggtga gcctcgcaga ggcgaatcgt gagaggagag cttctcgatc
     9421 ttggtgtcgt ctccgtagcg cacgcggtaa atgtcgcgga tttcatcgcg agaatagcca
     9481 tcatcgacga gccgcacgac gtagcgcgcg gcctgaacgc agcgcttgca cttccgactc
     9541 tccgaaacgc aacgggccac gctcaccggc tcaccgcagg gcgacaggag ctcgttgacc
     9601 atatcgatcc aaatccccgc gctgaacgaa tcgagcgagc tgatatcaac ttggggcagg
     9661 ctccgaatgg gctccttgga ggccttcggg gcgggcttga tctccggcgc gtcgccagcc
     9721 gaaccgctgt cggcctccgt ggagcgctcg caccctagcg acgcgaagac catgaaaatg
     9781 cagagtgccg tcgaacgcat gggcgaccgg atagtagcag acattccgaa gcgataccgt
     9841 cggtctagga ctcgctgtag ctggtgagtt gcgcccgaac gatgtcctcg ttgattcccg
     9901 ttcggatgta ttccttcagg caatcggcaa tcgtcaccat caagtcatcc attcccttgt
     9961 cctcgatcag ccgggaaagg agggccctgg actcgcggct gtcttcgtcg cgcaagaatt
    10021 tccgtacctc ttcgatggtg atcccgccct ggaaatcgat gtaagcgttt tccataaggt
    10081 aacgcgcaag ctcgttcaca tgaccctccg tctcactgtg cttcagtcat ctattagaca
    10141 cccgatcggc caaacggcaa gtgataaagg cccagttcaa gccctatcct gcttttctag
    10201 acgtcccgct cgtgtaatcg tgcccggcca tggagcccag aacccacctc ggagccgatc
    10261 cccgcttctg cggcaccttg ctcgacctgg gcgcgggttc cgcgcaggtc gccttgcagg
    10321 ccgtctccga aatggccgtc gacacgcagg gcctggttca cggcggcttc atcttcggcc
    10381 tggccgatta cgcggcgatg ctggcagtca acgacccgca cgtggtcttg gcggcggccg
    10441 agacacgttt tctcaagccg gttcggctcg gagaccgcgt gattgcgaac gccaggacgg
    10501 tcgtcgcaaa gggaaagcag caagaagtcc gagtcgaagc aacggtcaat tcgcagaagg
    10561 tgttcgaagg caccttcact tgtatcgtgc tcgagcgcca tgtgctcgag cgagttggga
    10621 gcacctaatg gcagaagacg gcggggcaat catcgggcga gtccttgcct ctcgaggggt
    10681 gaagcagctg ttcacgcttt gcggcggaca catctctccg atcctcattg gggcgcagac
    10741 gaacggcatc aaagtggtcg atgtacgaga cgaggtgagc gccgtgtttg ccgccgatgc
    10801 ggtcgcgcgc atgaccggtg tgcctggggt cgcagccgtg accgccggcc cgggggtgac
    10861 caacacggtg accgcggtga agaacgcaca actcgctcag tctccgttag tgatcttcgg
    10921 gggtgccgcg gcgactctgc tcaagggccg cggagcgctc caggacatcg accagatctc
    10981 gctgatggag ccgatcacca aatgggcggt ctcgatcaag aaggtgaaca gcatccaacc
    11041 gaccgtggag agggcgctcg acctcgcgca agacggggtg cccgggcccg tcttcgtcga
    11101 agtcccgatc gacgtcctct accccgaaga gatggtacgg gagatgttca tgaaagagag
    11161 cggcgtcaag gagggaaaga acctcggttc caaagcgctc gagctctaca tgcgtggtca
    11221 tctgtatcgg cagtttcacc aaccccacgt gagcatcccg ccgccgatcc gcgattcgct
    11281 cccgcctaaa ccgagcagca agagccacca cgtggacaag gttgcctcct tgcttcgcaa
    11341 ggcagagcga cctgcactcg tcatcgggag tcaggcgctc gtcaacacgc gcaaccccga
    11401 atcgatcgca ggagcagtga ctgccctcgg tgttccaact tggcttggcg gcatggcgcg
    11461 cgggctcctc ggccgacata gcgacatcca gtttcgccac aagcgaacgg ccgcgctcaa
    11521 ggaagcggat ctggtgatcg tagccggctt tccgttcgac tttcggatgg gatacgggcg
    11581 gagcatttcg aagcgcgcca ccgtagtcgc cgccaaccta agctccagcg aaatgcgaaa
    11641 gaaccgacga cccgagattc cggtccagat gcatgccggc gagttcctcc gcgagctcgc
    11701 caacacggtc ggcggggggc tcggcccgtg gagcaactgg ttcgacacac tgagggcgcg
    11761 tgagcgccag cgcgacgaag agatcggcga gcaagcaaac gccgggaccg atctcgtgaa
    11821 tcccctacag ctctttctgc gcctcgagga aaaaatggcc gacgacgccg tgctggtcgt
    11881 cgatggaggc gacttcgtcg caacggcgtc gtacatcttg cggcctcgca agcccttgag
    11941 ctggctcgac cctggcgtct ttggaacctt gggagttgga ggaggctttg cggtcggagc
    12001 ctcgttcgtg cgacccggaa aggaggtctg gctgatctat ggcgacggct cgagcgcgta
    12061 tagcttggcc gaaatggata cctgcgttcg gcacgggctt tcaccgatcg cggtgatcgg
    12121 cacggacgga tcttggtctc aaattgcgcg tgaacaggtt ccgatgctcg gcaccgacgt
    12181 cggcaccgtc ctgaggcgga cggcgtatca cgaggtcgcc cagggctacg gcgccatagg
    12241 cctgctgctc gacgatccct cgcgaatcga tgcaactctg gacgaagcca aagccctcgc
    12301 cgcctccggc aagccggttg tgatcaacgt gcacattgcg agcaccgact tccgcaaagg
    12361 ctccatctcg gtttgaacac gatggctact tggctccaac ggcggaaggc gcaaagccgg
    12421 ctgagagacg aagtcggcct ttggtaccat ccccagtaca aggccgaggc cttggtcgag
    12481 agcgcacgcg tgccggggat cgaagtcgcc cgcggcgaga agatcctcgg gtcattggcc
    12541 gccgaagggc tcatccgccc caagcaggta aggccggcgc cgctgatccc actgagggag
    12601 ctcgcgaagg cgcatgccga aagctacctc gaaaagacgg cgcgcccgga gtatctaggc
    12661 cgcattttcg gcctggaagc gcacgacatc gacgtcgacc caatcttgat cgctcaacgc
    12721 agacaggttg gcgggacagt cgcggcggcg cgctgggccg ttcgagaacc acgtcgcgtc
    12781 gcgttcaaca tcggcggagg ctttcatcac gccgaacccg aagccggagc cggattctgt
    12841 gtttacaacg acgtcgcggt tgcgatcaga gtactgcgcg ccgatggctt cgaggctccc
    12901 atcgcgatcg tcgatctgga ctatcatcaa ggcaacggaa acatcgtcac cttcgaggac
    12961 gacaaatccg tgctcacgta ttcgattcac ggctcgatct ggagtcatgt cgaagcggcc
    13021 gcggaccagc agtatcagct gccttcccgg gtcgatgacg cgacgtattt gagcaagctt
    13081 cgggacacgc ttcccgtatc tctcgaaacc attcaccctg gtttgatctt ttacgtggcc
    13141 ggcaacgacg tcctcacagg ggatcgtctc ggcgaattcg agctcagccg agcaggcgtc
    13201 ctcgagcgag attgcttcgt catcgagatg gcgcaaaagc tgggctgccc cgcggtcgtg
    13261 acgctcggcg gtggctatag cgacgacgcc tggcgtgcgt cgagcgactt cattcgctgg
    13321 ctgctcacgg gcgaactgag ggtgagcacg gagacgccta agagcctctt cgaacaatac
    13381 gcgcaagtcg ccatgcagct cgatccacag gagctccaag ggtccagcgg agagtggatt
    13441 ctcaccgaag cggatctcat gggcgatctg actgggccgc aatacaagtc gacccgcatg
    13501 ctcgactatt actcccgtca tggcatcgag ttcgcgctcg agaagtacgg cttggctgcc
    13561 gaagtccgag accgaggttt cacggatctg aaactagctg tggacccgac ggatccagaa
    13621 cgacagcacc tcacgcttca cgcgacgaaa aatggctcgg agcatctgct cgtcgaccta
    13681 gtccttcgac gtgtcacgcg ccctgcccca gagggactgg agccgccgga cgagatccgc
    13741 cttctctaca tcgaatggat gatgctgcag aaccccacgg aagcattttc cctgcgccag
    13801 ccccagtggc ccggccagga tcatcctggc ctcggggtcg gcgaaaaggc catgcacatg
    13861 ctctttcaag gggcacagcg cctcgggctg gacgggttgg cgcaccaccc ttcgcgctac
    13921 cacatcgcat tcatcggagg cggtcagtcg ttcttcctcg atcctgagct tcaagggcga
    13981 ttcgacgcga tccgggaagt gctggcgccg ctcgacctcg ctgaagccgc ctggaagatg
    14041 gaacgtggcg aggtgtgctg gtcagacggg gagccggtgg cctggatccc cgaggacatg
    14101 gtgattccag cgagcgaccg tttcttcgcg tatctaggct cgatccctta ccagcgaccc
    14161 cgggcagagt cgcaggcccg agccaaagcg cgcggcatcg tgctcgagcc tactcgccgc
    14221 acgtcgtaga cgcgggctcg ccagcgaaac gcgctgccgt ccactccagc atgagcggca
    14281 tggttttctc cgtagaatcg ttgtgtccgt atcgggggaa cacccggtag tcgacttcat
    14341 ttccgatctc gcaaaggcga ttctcgagaa cgttggtgag caagatgggg acgacccgat
    14401 cttgctgtcc ctggatgagc agaatcggaa cgccggtggg gacgttccct ggagagttct
    14461 catcgagacg ggtgacccag gccaggattt cgagagaact catcgcaaac ccggcaccgt
    14521 cgaacgtcgc tgcgacttcg ccgaactcgg cgccgcacgc ctgaggtgat gttcgcttga
    14581 aactcgctgg cgggcgcggc gctgatcacg ccgagaacgt cgagcccagg cgcccaggtc
    14641 ggcgcgattt cgccagcaaa tagcgcccca tctccgccct gcgaacggcc ccagattacg
    14701 acgcggcttc ctgcgcgccc ggtatcctcg gagcgcgaac gcggtcaaat gtaatgactc
    14761 atgcgacctt aaaacagcac ggcgccccgc agaagcgagg cgccgtggtt agagggttcg
    14821 taagccgagt tttgtagccc gcgctcgtcg ccgaggcgcg ggtcgtgatc attcttctgg
    14881 gaccgatgtc gccatcggcc tcgagcgacg ctacccggag acttgggcgg gaccgccctc
    14941 aacgcctccc tatttcgtct tgcttcggat ggggtttgcc gtgcgaccga cgttaccgcc
    15001 agcccggtga gctcttgcct caccctttca cccttgccgc gcggcctttc ggcggcgagg
    15061 cggtttgctt tctgtggcac tttcctcggg gtcaccccca ccaggcgtta cctggcatcc
    15121 tgccctgcga agctcggact ttcctcccgc gtgcagaagc acgccagcga tcacgtgaac
    15181 ctgtctaacc gcttgagggt aagtccgggg acggaccctt gcaactacag cgcctgtatg
    15241 tccgcggacg ccggcgagga ctctggcagc ggctcccgga ctagatcggt gagaatgtag
    15301 gtgagcgcca agagcacagc gacggtctcc cagatgtact gctcgtcgta ccagcctatg
    15361 aagaactgca gaatcagacc ggtgatcccc atcaggatga acgcgaccga atagcgtagt
    15421 cgctcgattg cgagcggtgc catcaagatc acgaaactat agtagtagca agtcagctcg
    15481 aagaacccga tgatcagaat gctgcttgct gcgagcaccg cccacccggc gaggcggcgg
    15541 ccggcaagcg cgagcagcac gaacatcccg agaagaatga gcgcgtgaag ccagcgcctg
    15601 tcgtgcttgg tttccttgcg cgcctgtttc cacttggccc agggatcgtc gaggctcgag
    15661 tccttcgtaa atcgaccgac cgtacttgga cggtaggaaa gaatcgtagg cagccccatg
    15721 tggttcgtca gcggggtttc gctgtgtttg atcgtgttcc cgagaaactt cgaatgcgca
    15781 tctacgccat tgatcgcgac gctggcagtg accagaacga gcaagcccag aatgcctccg
    15841 aggatgaagc gcttgtccgt cgagtccagc cagggacggg cgattttctt ccggcgaatg
    15901 aaccggtcga tcaagcttgc catgggccca cctgcgagcg cggccgggaa caatcgcaag
    15961 agcgcgctcc atgagagcgc gaaacccgag agcagaggaa agccacgctt caagaggcag
    16021 accgctgcca ccgccatgaa tagccaaggc acccgcgcaa agcttccgcc cgtccagaag
    16081 taagcccaag gataaccggt tccccagacg agcacggcga gtgcggtcga acggagaccg
    16141 aatgcccaga ggatcatgag gaaggcgccg gcatagagcc ccaaatcgat catcgtgtag
    16201 cgtaggattc gttcctggaa ccgcttcaag tcgtcgggcg caacttgggc ggaggaaatt
    16261 cgaggcacct gttcttccca gccgatgttc gtaagaaacg cggcgaccat gttggaaatc
    16321 gggctcgcgt tgtacccgtg gtccatcaac atgtctttcc accaggccga gcccatcacg
    16381 gtgcggaagg atcgtgtgtc ctggcgaaac gcatcccaac gttcctgagt gaagtgctcg
    16441 gcgcaatggg actgataggc gagcgcatcg gctttgctga cgaagtggag gcgattgtcg
    16501 actagatcac gaatcttgcg cttctgcagc tccgcttccc cacggtcctc gtagtcggct
    16561 gcgagcacgc actggtagag tcgctcgtac tcgttctcct cgaagtactt cgatccgacg
    16621 tagtagtgga acgtgtccca gtagtgcact acgcgggagc tgtggaagac accgaagttc
    16681 acccaggcga tcgcggaagc gacgattatc cagacgagtg cgccctgctc ccaccaagtt
    16741 cgcccctccg gctcccgttc ctgcctggcg agccaggctc cgcgtgctgc ccatgcgact
    16801 acgaggattc ccaggacgta cacgccccaa ctcgcgaacc agggagcgag tcgtgcaccg
    16861 tagaggcggt agccggcgag cccgagcatt gcgacggaca aagccgaggc cccgaccagc
    16921 gccttcttgc tttcctcgat tcgaaagagc gccacgaaaa acaagagccc gagaacggcg
    16981 agcgcgagcc gatatcgatg gtcgcgcttc tccaggatct tgttccacca ccacttggag
    17041 gtgttcttca cctcgacggg cggcggccaa gccgcgggcg tttcgcagaa gacctgaatc
    17101 tcagcaaggc tgaagtaacc atccccgccc tgcccgcgca ctcgaacgta gcgcccctga
    17161 ccttcgagtc cgcgcgcgct gcggcgccgc atcccctgtc cggaaaccgg atcgcctacc
    17221 caaagagggc tgaaattcct tcgatcctcg gatagctcga cgatgtagtc gtcgttgttg
    17281 tcaccctgga ggtcgaccgc ggtcacacgg gtggtggcgc cgaggtcgta gatgacttgg
    17341 ctttccgcgt cggcgagcac cgaggtgagc tcgctttccc aagggtcgcc ctctcctgct
    17401 ttgacgccat cgttcaggcg ctcggaatgg gtgacacctg gccgcgcaac cggtgttttt
    17461 ccggcgagca ggtttccagg gggacaggct acctcttgcg ccgcggccgg agccgcagtc
    17521 aacacaaaaa gaaagcaaag aacggggatt cggtacacga tggcgcgaaa cctacgcaaa
    17581 ggagcccagc ctgacaagcc cacgtcgagc tcctccgcgg attcgtcgtc gggggcgaga
    17641 cccctctccc aattcccctc tagacttccc gcccggccgg tcttattggt ctcgtgatgg
    17701 gttcaaattc ctcgaagaag gctccggctg catcgacgga aaccgatatg gttctcgtct
    17761 atggccagtc cgaggacgga cagcgttacg gcgtgattcg ccagcgcggc gctgagattc
    17821 aagctgggac gatgcgcccg cttcaagaag gcaagccgat tctcggcgag gtcgttcgcc
    17881 tcaagccccg cgaagagtcc ccggccctgt tcgacgtgga ggtgcagcac cagggatcca
    17941 gcgcgcgcgg ccggccagcg aaggtcgcca gcgaggatta ccggcgtggc tgggaatcga
    18001 tctggtctaa caagcggcgc aaccaagcgc tgaattaaaa agggtgccgc ccatcggcga
    18061 cacccttccc cctgctgttc ttactgtccc tggagtggcc cttaggtcac gtaatccttg
    18121 agtcgctcga catctcgata ctcctgaagc ctggcgaggc ccgccgtctc cacctggcgc
    18181 acgcgctctc gagtgaggtt catgatctct ccgacctctt cgagagtgat gccgccacgg
    18241 tccgcgacgt cgagcgcaca ggtttcggtc atttcccaga tctcgatgtc ggggaagttg
    18301 agcttgatcg aaccgcgcac cggatgaacg tcgatgaaca ggtggtactt gcaggatacg
    18361 aatgggcacg gccgttccat gtccttgcat tccaagcggc ttctcggttt ccagtagtcg
    18421 gtttccggga agagctctcg gccacgattg agctccgcct tgctcagtcg cctaatcgaa
    18481 atcgtgcgag cccgtgcgcg tgagcgtcgt tttctacgac ccctacgctc gtcggttcca
    18541 ggctcgtcga gtgcgtccgc gagtgactca cgctcctctt ctggattgga atccccgtcg
    18601 acaacctctg cgcttgctgc gtccttcatg acatctctcc ccctcgaatc cgtcagaccg
    18661 ctgcccgcgc catacgggcc cgcatagtca ggcgccgctc gatggcctcg agcttttgaa
    18721 cgagctcctt gccgagctcg cgaacgtcat tgagcgcatc gccgacgccc aggtcgtcgg
    18781 atccttcgcg ctcttgctca tggaccgcat cttcgatcgc ccgatacaca cgcttgagtt
    18841 gccggcgcag ggcatcgatg tcgccacgaa cgaagaggaa ctccttctgg atttcctcca
    18901 tcgtgaaccc ctgatgcatc agacgtcgga tgtgatcgat ttggcgaacg gccgtcgccg
    18961 gatacagccc ctgggagccc ctgtgcttgc ccttgcggcc gacacgaacg ctccggggca
    19021 gtagcccgag ctggacgtac ttgcggaacg tcgcctcgct cagtctcgag ccacgggcgg
    19081 tgaaggtctc gacgatctgc tggacgctca tgccctcggc gtgcgccctc tcgatctgct
    19141 cgagctcacc gtccgaccag gtcctggcct ccgattcccg atatctcagc tgctgtcccc
    19201 taggtagagt catttgaata acttatagcg aatgtattcc attcaaaata attaggtcaa
    19261 cggcaaaata tataaagtta taaaaccatg taattattga attttatgga ttccaaaatc
    19321 caacctgggt cgtttttgaa ttgcttttga gatcggcgta tcacctctga gatcggtttt
    19381 gcggggggaa attgacggcg acactcgact ccagtacctc ccgacaggcg catggattca
    19441 cgggcgtttc gacggtttcg gcggaacaag ggggctctcg ccggcctggt cctagtcgtc
    19501 gcggtgagcg tcctcgggct tctgggcccc tggttggcgc ccttcgaccc agtcgagcag
    19561 ttcaccgata gcctgctgct ctacaacggc ctgcctgcgg gtccgggggt cgagccgggt
    19621 cactggctcg gcggcgatgt gctcgctcga gacgagctga gccgcctgtt gagcggcgct
    19681 cgcgtttcga tgctggtggc ctatctggcg acggccttgg cggtgctgat cggcgtgacc
    19741 ctcggactga tctcgggcta cgtcgggggc cgcgtcgaca ctttcctcat gcgaatggtc
    19801 gacgtcgtgc tttcgatgcc gttcctcttg atcgccatcg tcatgcagcg cgtctgggat
    19861 gcgccaggga tttggacaat ctgcatcgtg ctcggcgtgc tctcctggac gaccttggcc
    19921 cgtgtgacgc gtgccaagac gatggaagcg cgcgagctcg actacattca ggccgcgcgt
    19981 gcactcggcc ttcgacactt tcgaattcta gcgcgtcacg ttctcccaaa cgtcatcggt
    20041 ccaatcatcg cgatcgcgac gattctagtc gcgcgaatga tcatcgccga gagcgctctc
    20101 tcgtttctag ggctgggcgt ccggccgccg accgcgagtt ggggctcgat gctcaatgac
    20161 agtcagtcct tgctgctcgg cgttccccgg ctgacgctct accctgctgt cttgatcagc
    20221 atggcagtct ttggtttcaa cctttttgga gaagggctcc gtgatgcgtt cgatcccaag
    20281 gactgagcgc aacgccccga cccgctggtt aggcggcgcg gccgcgattc ttctagccgg
    20341 cctgctcgtc gcgtgggtcg ttgcgcactt cccaaaggct ccgctggggc ctcgctacat
    20401 cggcgcggga caccacacgc ctcagtcggg gggcacgttc atcttcgcgg caggcagcaa
    20461 cattcacaca ctcgatccgc atttcgccta cgacacactc tctaccgcgg cgtgccgcct
    20521 catctatgac ggactgctcg attacgacta cgaagggaac atgatcccaa gtcttgccgc
    20581 cgagcttccg gagatctccg acggcggccg cgtcttccgg ttcaagttgc gcgacggcat
    20641 caaatttcac aatggacgcg agatcaccgc ggaggacgtt tcctggtctc tgcaccggct
    20701 gctctcggag tccgttgggt ctcctggcta tccgttcttc aagtcgattg tgggagcgca
    20761 gcagtatcac gctggggagg tcgagcagat tcagggaatc gtcgtcatcg atgagaagac
    20821 gatcgagatc caactcgatg aagcagatca gaccttccta aatgcgctcg ccatgccatt
    20881 cgcctaccct atcgcgaaag agcacgtcga gaagctcgaa gccgaagagg gtcgcgacgc
    20941 ggtgggccgt catcccgtgg gcgccggccc attcaaattc tcgcgctggg agcgaggcgt
    21001 ccaggtcgag ctcgaacgct tcgaggacta ctgggcaccc gctgctcggc cagaccgaat
    21061 ggtcttcctc gaaaacatct cggcggatgt cgcaagcgcc cgcttccgaa acggcgatct
    21121 cgacatcctc taccggccga gcaaggtcca ccgactgttt ctgcgagagt ccgaggcctg
    21181 ggcgccttat ctcgcagaag cgccgagcgc atccctcttt gcgctgggtc tcaactgcga
    21241 gctcccgccg ttcgacaacg ttcatatccg ccgcgctgtg gccttcgccc tagaccgttc
    21301 gaagctcgaa aagctcgatc cgggtcaggt gatcgcagcg actcagatct taccgccgat
    21361 gcttgcccct tacgatcccg agcttcctac aaagcagcga ttcgatctcg cacgcgccaa
    21421 agaagagatg cgtttagcgg ggtttccaga cgggctcagc gagccgatca cgctctgggt
    21481 ccgcggcgag agcgaggtgc gcgtggcaca gctgttccaa caggacctca aagcgatcgg
    21541 catcgagatc gagctcaaga tggtttcgtt cgcaacgtac ctcaaggaga cgggaaagcc
    21601 gcgcgttgcc caggcggcct tcacaggctg gcatcaggac ttcccggatc catcgaactt
    21661 catggacatc ctcttccaca gccgctctat ccaccccgaa aactccgaga atcgctcctt
    21721 ctaccgaaac cctaagctcg atgagatcct cgaccgtgca cgcccggaga tcgatcgaga
    21781 gaaacgccta gcgctttacg ccgaggccaa cgcaatccta gccgacgagg ccccctgggc
    21841 gtttctctac tacccggtcg acatgttcgc atggcaaccg tacgtcaaag gcttccgtcc
    21901 gcatcccgtc tggctcaacg agtatcgaaa catctggctc gacctgccca ggagccgcgt
    21961 gagtcaggcg atctactccg aggaggctcc gtgattcgat tcatcgcgca acgagctctc
    22021 tggggcatct tggtagtcat gtccgtcgtg acactggtct tctttctcat tttcgtcgct
    22081 ggcgatcccg cattgaccgt tctcggtcct caggcccgcg ccgagcagat cgccgacttc
    22141 aaagccaaga agggcttgga tcggcccgta cccgagcagt tcctcagcta cctaggcgtc
    22201 atgccctgcg tgcgcaaaga ctcgtccaag tacgagcgag acgatggctc gcgcggcttc
    22261 tgtggcatct tgcagggcga cctcggcgag tcgtactcgc accacgaatc ggtggcgaac
    22321 gtaatcggat accgcctccc tcgcacgctt ctgctcgctt cgcttgcggt ccttttcgag
    22381 ctgatcatcg gtctcaccgc gggaatcatc gcagcgctcc gacgcaaccg ttggtccgac
    22441 acgatcatca tgttcgcgac gttcgtcggc atcagtttac ccacttacgt gacgggccct
    22501 atcgcgcttc tgctctttgc gttcctcctc ggctggtttc ccttcggggg ctacggcgtc
    22561 gatcccctcg atcacgtgta ccacgcgctc ttgccctctg tgattctggc aatcgccgga
    22621 gcagcaacct atgcccgcgt catgcgcggc gagctcatcg aaactctaag gcacgatcac
    22681 gtacgaaccg cgcgagccaa ggggatgccc atggcgcagg tcgtgatcaa gcacgcgatt
    22741 cgcaacgcgc ttctcccgat cgctaccttg atcggtctga gcctgccatt cctggtcggc
    22801 ggtgccatca tcacggagaa gatcttcgcc tggccgggtc tcgggcttct cacgattgag
    22861 tcgatcgaat cgctcgatgc gccagtcatc atggcagtcg ttctgatgtt cggcatcgcg
    22921 gttcagatcg gcaatctgtt ggcggacatt gcggtcgccg cgctcgaccc acgtattcgg
    22981 ctccgcgact agccccttgg cttgatgctc acctgaaaga ttcgccgtcc gtcgtcgatc
    23041 cgtggagccg atacctgcat cgcgtcgatc ttgatcttct tgcgccgcga gtcgggaagc
    23101 ttgatgaagc agaccccatc acccgatacg ccctcgatcc tgcagagccc gaatttagcg
    23161 tgctcgatcc aatccccttg ctcgggcttg tgctctagcg cagctcttgg ctctgctctg
    23221 gctgacaggg ccgccgccat cgcccaggac acaccctctg aacgaggcgc catcggcgac
    23281 acggccccgc ccccggagtc cggcgcagtc gcctcggtcc agagttgaag accggtcgct
    23341 tcgtcattcg cccgttgcag caccagctcg tcgaagcaat cgatccgaaa ctcgagcgca
    23401 aacaccgaac catcgaccac gtgccctcca taggtcacgt cgcgcccatt ttcacgcaga
    23461 gagatggtca catgcatgtg ccaaaatgga gctccatcgc gctccgatag attgccttgt
    23521 aggctgagga tctcgcagcc ctcgaagcga tggggcgctt cgtaacgtcg ctcggcctga
    23581 ttgtattcgg tgagctcgat ccattcgaac gcgcccatcg cgcttaccca tgccgtgcga
    23641 aggcgatgct cgtcgaccag cttgcgaaac gcgtcctgaa gcccggctcc acgcgggact
    23701 cgaccgagaa tgtgtcgaga cagcggcgtc tcgaatgaca acagctcgtc ggaagaggtc
    23761 acgacgccgc ttgcgtgagg cgctcttcct tcgcgtcgac ggcgtcgacg aagagcttga
    23821 agtactcgcc cgcgctcaag agagccagag cgaggctgag gtaaagaagc accaggccga
    23881 cttgtgcgaa gtcaaactcg gtttggtaga atccgaaata gatcgtgtag cggtgatgca
    23941 cgatgagcat cagaatggcg accatctgaa gtgcagtctt gtctttgccc gttcgcatcg
    24001 cagccatcac tacgccctca ctcatcgcga tcgtgcgcag cgccgtgacc gcgagctctc
    24061 ggccaagcat gatgatcacg gcaaccaccc cgagaaacgg caggcgaccg agatacgcaa
    24121 gagagatgag agtcgccatg acgagcagct tgtccgccaa gggatcgagg aactttccga
    24181 gcacgctgat ttgaccccac ttacgagcca ggtatccgtc gatgagatcg gtgagcgcgg
    24241 tcccgatata gagcattgca gcccagaagc tcgccttcgg cgtgccgtcc cagagcagcc
    24301 acagcacccc ggggatcagg gcgatgcgca acatcgtcag caggttgggc agattgatgg
    24361 cgtcctcgcg aatcgaagga cgtttgggag ttgcctcttc catgcagcgc gttgtacccc
    24421 aggttgccac tcccgtccgc cgtcgaagag gatactcctt taggggtaag cggctgaggg
    24481 gcactgcgca tcggatgctt gcactgcgtt cgcgctctcg acacactccc cgccaccggg
    24541 aatcgcatgc tggagctcac gcggaacgtc gcagaccggg cgcgaacgac cctaggcatc
    24601 gcgacccaaa atttcgcggt tcaggatgcg atcgccgtcg cctttcacgc ctatttgttc
    24661 attcgcatca gcctggcgcc cagtagccca gacgcgatcg tagcgggccg cttggccctc
    24721 gcactactcg tcgtcaccgc ctgctcgatg atcctcagcc gtggcgagct gatccgccat
    24781 cagaaagccc gcgcgctcac ttaccgcttg ggtctgtttg tgccgatggt gctgtcgtac
    24841 ttcgagatgc ggatcctgct ccccgcgctc cacccggagc tcatggacta ccggctattt
    24901 gccatcgacc tctggctcct cggcaccacg ccatcggtat ggatgcaggc ttggaacctc
    24961 cagccaatcg tcgaatggtt ttcgttctct tactacacac acttcttcgt cttgatcatc
    25021 atcctcattc ccgcgctcat tttcgggaag gggcgacgtc ttcaagagct gatggcgggc
    25081 ggcatgctga tcgtcgccct ggggcacttc ctctacacgc tggtccccgg tgctggcccc
    25141 tacgccaccc tggggttcgc tgagccgttg catggcggct tctggtggaa tcaggtcagg
    25201 atcacggtgg caacggcggg cgctcaactc gatatctttc cttcgctcca cacggcgtgg
    25261 cctgcgtatt tcacgctgca tgcctacgcc caccgaaagg tgcagccttt ccgagccctt
    25321 tggcccgttc tcgggttcat cgctttgaac atcatcatcg ccacgatgtt tctccgctgg
    25381 cattggttca tcgacgtagc gatgggcctc gtgctggcgg tcgtagcccg ccggttcgcg
    25441 gtggccgttg cgacgcgcga agaggaacgc ggaagccgca gcgacccccg ccagcccgtc
    25501 tgggagcggc tctactagcc gcccttggag aatcgcccag cgtcgcgcag aaggctctcc
    25561 ggaaccggga tctccggcgc ctcatccatt gcccgaaacc tgcggcgcag ccaccagatg
    25621 aagcctccca tcatcatgag cggcacgaga gtcaggaaga tcgtggtgtc cacgaacgcc
    25681 tgcctgttct cttcgagcgg cgatgcgcac gacgggcagg cataggcgag gtcggccgcc
    25741 caggcgaccg aaagcagcag agcggagacg caagctcggg ctaggcgaat caaaggtaca
    25801 cctgccaata gaggacgggc caaaccccga cgacgaagta ccagaagatc tgcgccggcc
    25861 aaaaagtgaa gcggtccaga ttgccctcgg aaagccggcg ataggtgtag agcaagatga
    25921 tgaacccggc gaccacgtgc agcgcatggg taccgacgag gagatagaag aagcctccga
    25981 gttgcccgga ggtgagtgtg agaccctctc tgatgagcgc gacccactcg gctccttgaa
    26041 agagcacgaa gaagcctccg agccctatgg cgagcagtaa caggcgcttg gtgttgggag
    26101 agctccgttg gaacgccctg ttcgcgaaat agagcgccac gccgctcgcc aagagtgccg
    26161 cggtgttgag cgcggtctct tcgactggaa ggcgtggttg tccaggaggt ggccagccac
    26221 cgatggcatt gctcttgccg atcacgaacg cgctgatcaa gcccgaaaag aacatgatct
    26281 ccgtcgcaac gaagatcagc atgccgagca caccgtttgg aaggactggc gcgcgcctgg
    26341 ctggaacgcc gtacgggcgg cctagtgcat catcaggcat tggctggtcc tcctttcctt
    26401 agtgctgggc gtcgtggtgt tccccggcgt gcgtcttggc ttcgctctgg gattcgaggg
    26461 cctgttcgac ttcggtttcg gccgcaacgt tctcccagtt ctgtcccttg tgctccatga
    26521 catccggagc cgtacccgca aagaacagca gcatgagcat cagcatcgtg gcgagcaggt
    26581 acgagatgta cttcttctcc acgttgaggt gcatgaaatt cgccgcaacg agatacgctt
    26641 tgacgaccgc gattccgaat gcggtcatca aggtgatcat ccaggccttg aggaaaccaa
    26701 attccacgtg gggggcgacg atgggcccca ggacactcag gatgagcagg accagaagca
    26761 cggcccaaat tttaacgtag tgggcttggc cgtggtcgtc tgcgtgactt tgatgttcag
    26821 ccatgatcgt cagtcctcac ttagcgatgt agagcagcgg gaagaggaag atccacacga
    26881 tgtccacgaa gtgccagtag atgccgatga gctcgacgcg ctgaagctct ttgcccctac
    26941 gcgcttcgtt cgcgatgatc agcatgatga tgcaaccggc gatgacgtgg agcgcgtgaa
    27001 ggcccgcggc tgtgtagtag aagtgccaga agccattggc ggttatcgtg tagccgtgcc
    27061 ggatctcgcc ggtccattcg accgccttga tgcaaaggaa gaccaggcca cccccacacg
    27121 ttccgagcag ccacttggcc gccttcttac cgtccccgtg ttcggccgcg tggtgcgcta
    27181 gcacagcaaa cagcgatgag gtcaggagca cgaaagtgtt cgcggcgccg aaccaggtgt
    27241 tggtgtgaga cgcgtattca gcccactcgg gatggccgag cctgtgcatg atgtaggaag
    27301 ccagaaggcc tccaaaaatc acgatctccg agccgatgac ccaccagacg gctagcttgc
    27361 cggtggcgat gcccgtggcg cttcgggtcg ttgctatggg tttggtcatg ctcatcggtg
    27421 cctcgtcatt cggcgggggc tgtgtccggc gggacgtctt gcggccagta gtcctcgtcg
    27481 cgacccggca cgctgtactc gtagggacct cggtagcagt tcgggagctc cggccaattg
    27541 ccatgaggcg gcggggactc cgcagtccac tcgagcgtgt tggctttcca tgggttcttt
    27601 cctgcgggct tacccttgat cagactcgtc acgaagttga acaagaacac ggcctgaaag
    27661 acgagcatca ccaagagtgc gctcgttgcg aggactcgga ggtcttgaag gcctgcaagg
    27721 tcgggaaagt tctggaagtt gtaaatgcgt c
//
LOCUS       DASRZF010000355         3550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3706305, whole genome shotgun sequence.
ACCESSION   DASRZF010000355 DASRZF010000000
VERSION     DASRZF010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3706305"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VFG22_15840"
     CDS             <1..349
                     /locus_tag="VFG22_15840"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET6417769.1"
                     /translation="HPLAVAEALRALGPETVIVKRGEYGALLFLDEDIFCAPAYPLKS
                     VADPTGAGDSFAGGFLGYLAENGHTDLRQAVICGSATASYCVQDVGVGALQELSRERL
                     EKRIQDFRELTCF"
     gene            412..654
                     /locus_tag="VFG22_15845"
     CDS             412..654
                     /locus_tag="VFG22_15845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417770.1"
                     /translation="MNLDELKGTLRGLVRKTIETRFSGANYATLAQSRGYADGYMRAL
                     LDAGLVDQKQLLELVNAERRLFVDEANKLSDATRAA"
     gene            complement(690..2084)
                     /locus_tag="VFG22_15850"
     CDS             complement(690..2084)
                     /locus_tag="VFG22_15850"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET6417771.1"
                     /translation="MELADGRPRVVILGGGFGGLAAARALGGAPVRVTVVDRSNHHLF
                     QPLLYQVATALLPAPDIAAPLRNLLSKHDNIRVLLAEAQRIDIEKRVVMLDHAEPLEY
                     DFLIVATGLKHNYLGHPEWERHAPGLKTLYEALEIRSRILLAFEAAERLTDPDEKRAF
                     LTFVVVGGGATGVELAGQLSEISRNALRRDFRTIDPSSARIVLIEGADRIFRDYHPSL
                     SARAQRDLESLGIEVRTSTFVTDIDGDGVTVGEGERIQAHTVLWAAGVRASSLAEQLG
                     AELDNMGRVKVTPYCTVPGHDEIAVLGDLIHFPRKKDGQPLPAVAQVALQSGKLVAQN
                     IVRRTNREAPRPFEYFDKGLMATIGRNRAIVESRGFKMSGLPAWLAWLFIHIAFLVGF
                     RNRVAVLSQWIWAYVTNKRASRVVFSHPKEQGPTVLEPRPSLEPSAAEQATAEMGVPS
                     ARPSGAWAWSRSDD"
     gene            2300..3217
                     /locus_tag="VFG22_15855"
     CDS             2300..3217
                     /locus_tag="VFG22_15855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET6417772.1"
                     /translation="MTRRRIIEPGRVWALSRRTTRRYFLLNPDEARQIEQAYWYCLGH
                     AAEVHGVVVHAACLMSTHSHEVVTDVRGQLSLFLQAFHRHFALCTKAIRGWPGEVFDK
                     RSTGAHELLTPEATLESLAYLIANPVEAMAVRYARDWPGAQTLPRDIGTRVVTVPRPP
                     YYFNPDNAEWPQAIELRMQMPTALECDYGTELAQERIAQRVRDREHRAWQESKRTGIP
                     FMGRRRLLRQAHTRRALGHEVSGSLNPQFAAAGNREAASAATKRIRAFNAQYDEALAA
                     WTSGMRTALFPQGTWWMRVHHGARCGPAP"
BASE COUNT          642 a         1142 c         1138 g          628 t
ORIGIN      
        1 tcaccccttg gcagtcgcag aagcgttgcg cgcgctcggt ccagagacgg tgatcgtgaa
       61 gcgcggcgag tacggcgcct tgctcttcct cgacgaggat atcttctgcg cgccggctta
      121 cccactcaag tcggtggccg atccgaccgg cgcgggcgac tcgttcgcag gcggctttct
      181 ggggtacctc gcggagaacg gccacaccga tctccggcag gccgtgatct gcggctccgc
      241 tacggcgtcg tactgcgttc aagacgtcgg cgtaggcgcc ctgcaagagc tcagccgcga
      301 gcgcctcgaa aagcgaatcc aggacttccg cgagctcacc tgcttctagc ccgccgattt
      361 cttgaccctg gcccctacaa atcgacactg cgggctgagg aaggaacggc catgaaccta
      421 gacgagctca aggggaccct gcgcggcctc gtgcgaaaga cgatcgaaac acgcttcagc
      481 ggagccaact acgcgactct cgcccagtca cgaggttatg ctgatggcta catgcgtgcc
      541 ctgctcgacg cgggcttggt cgatcagaaa cagttgctcg aattggtcaa cgcggagcgt
      601 cggctcttcg tcgacgaagc caacaagctc agcgacgcca ctcgcgcggc ctgagcacga
      661 gctcgttcgt tccggagcgg acgggatcct caatcgtccg agcgcgacca ggcccaggca
      721 ccagacggtc gagcgctagg aacgcccatt tctgccgtgg cttgctctgc cgcgcttggc
      781 tctagtgaag gtcgcggctc gagcacggtc gggccctgct cctttggatg gctgaacacg
      841 acgcgcgaag cgcgcttgtt ggtcacgtac gcccagatcc actgcgagag aacggcgacg
      901 cgatttcgaa agcctacgag gaacgcaatg tggatgaaga gccaggcgag ccacgcaggc
      961 aggccgctca tcttgaaacc ccgcgactcg acgatggctc gattccgacc gatggtcgcc
     1021 atcagacctt tgtcgaagta ttcgaagggc cggggcgctt cacggttcgt gcgacgcacg
     1081 atgttctgtg caacgagctt gccgctctgc aatgccacct gggcgacggc cggaagcggc
     1141 tggccgtcct tcttgcgggg gaaatggatc aagtcgccga gcaccgcgat ttcgtcgtgg
     1201 cccggtaccg tgcaataggg agtgaccttg acgcggccca tgttgtcgag ctcggcgccg
     1261 agctgttcgg cgagggagct ggctcggacg cccgctgccc agagaaccgt gtgcgcctga
     1321 atgcgctcgc cttcgccgac tgtcacgccg tcgccgtcga tatccgtgac gaaggttgac
     1381 gtgcgcacct cgatgccaag ggactcgagg tcccgctggg cgcgcgcgga gaggctcggg
     1441 tggtagtcgc gaaagatgcg gtccgcgccc tcgatcagca cgatgcgagc gctcgaagga
     1501 tcgatggtgc ggaagtcgcg gcgcaaggcg ttgcgtgaga tttcggagag ctgaccggcg
     1561 agctcgaccc cggtcgcgcc gccaccgacc acgacgaaag tgaggaatgc acgcttctcg
     1621 tccgggtcgg taagccgctc tgccgcctcg aaggccagaa ggatgcggct ccgtatttcg
     1681 agggcctcgt acagcgtttt gaggccggga gcatggcgct cccactcggg gtgaccgagg
     1741 tagttgtgtt tgagacccgt ggcgacgatc aagaagtcgt attcgagtgg ctcggcgtga
     1801 tcgagcatga caacccgctt ttcgatgtcg atgcgctgcg cttcggcgag gaggacgcgg
     1861 atgttgtcgt gtttggacag aagattccgc agcggtgccg cgatgtcagg agcggggagg
     1921 agcgcggtgg ccacctggta gagcaagggc tgaaagaggt gatgattgga gcggtcaacc
     1981 accgtgacgc gcaccggtgc accgccaagc gcccgtgccg ccgcaagccc accaaatccg
     2041 cccccaagaa tgacgacacg cggacgtcca tcggcaagtt ccatcccgac ttcatcgtcg
     2101 ctaaccccca ccctcgcaag tggatcccac cgacgatggc tcaaacgccg cagccccctc
     2161 ggtattcccg tgtgttctcg ggggctgacg gccggcagcg gctcttgacg agccggcagg
     2221 gcacagcgcc tgcgtgagtg gtaatccttg agcaaactcg gccgcgggcg gcggcacgct
     2281 tttcgctcct gtcctagaca tgacccgacg acgcatcata gaacccggca gggtctgggc
     2341 gctatcacgc cgtaccactc gccgctactt tcttctcaat cccgacgaag cccgccagat
     2401 cgagcaagcc tattggtact gcctcggaca tgcggcagag gtccacggcg tggtcgtgca
     2461 tgccgcgtgt ctcatgtcga cgcactctca cgaagtcgtc accgacgtgc gtggccagct
     2521 ctccttgttt cttcaggcgt tccatcgcca tttcgccctc tgcaccaaag ccatccgggg
     2581 ttggcctggg gaagtcttcg acaagcgaag cacgggagcc cacgagcttc taacacccga
     2641 ggcgaccttg gagtcactcg cctacctcat cgccaaccca gtggaggcga tggccgtgcg
     2701 ctatgcccgg gactggccgg gcgcccagac gctgccccgt gacatcggca cccgagtcgt
     2761 cacagtgccg cgacccccat actatttcaa tcccgataac gccgaatggc cgcaggccat
     2821 cgagctccgc atgcaaatgc caacggcact cgagtgcgac tacggcaccg agcttgcgca
     2881 ggaacgaatc gctcagcgtg tccgggaccg cgaacaccgg gcctggcagg agtccaagcg
     2941 caccggtatc ccgttcatgg gccgacgccg tttgcttcga caggcacaca ccaggcgggc
     3001 ccttggccac gaagtgtctg ggagcctcaa tccgcaattc gcagcggctg gaaatcggga
     3061 ggcggcgagc gcggcgacca aacggattcg agcgttcaat gcacagtacg acgaagcgct
     3121 cgcagcctgg acgtcgggca tgcgcaccgc gctcttccca caaggcacct ggtggatgcg
     3181 agtgcaccac ggagcacggt gcggacccgc gccgtagcgt cacgcgtcga aggggattga
     3241 ccccggtgag cacacgccag gttcggagcg atgccccgag acgcgggggt gcgccttcaa
     3301 ggtctagcga tgcgcgccgt agagcaatgg agggtcgaag acggacacga tcgggcgttt
     3361 taggctcacg gcgggggagc tcatagcggc cgcgagtcgt ggctcgtgcc tcgtctgatc
     3421 gtcattctgc tttcgcgtgc cgccaacctc ggtgggtttc ctttattctg ctttcgcgtg
     3481 ccgccaacct cggtgggttt ccttagtgcc tcgtctgatc gtcattctgc tttcgcgtgc
     3541 cgccaacctc
//
LOCUS       DASRZF010000356        28113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_270177, whole genome shotgun sequence.
ACCESSION   DASRZF010000356 DASRZF010000000
VERSION     DASRZF010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 28113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28113
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270177"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..729)
                     /locus_tag="VFG22_15860"
     CDS             complement(181..729)
                     /locus_tag="VFG22_15860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417773.1"
                     /translation="MHDGRFDPSGFYEFNLNAGMVRTRTGERVAIVSEDVLSSLVAAA
                     ARDGDLTPLRRLGELLGEQVIGGLDRPASVLSPEAVLGHASAVTSLFGWGRLTFERWG
                     SALVIVLRDKPELDEDELGAAALLGGMFSEISQRQVSCVPTGDSKFIMVDFEVAETVW
                     GWFKDGADLPAIVGMLETKRAS"
     gene            778..2118
                     /locus_tag="VFG22_15865"
     CDS             778..2118
                     /locus_tag="VFG22_15865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417774.1"
                     /translation="MPRVKSNLACVVLAISLQAQAARAQGDLLGADEMAEAIVSMTSP
                     VWTGPRTPPPDRVGRPARASQVLHSTNSLLAVHADPEVDPLVVRRALRALEYARGRLD
                     AMGWPSPISDGNLGGGPELDLYLSGELPPDAYFDGLARWSYLDRGSAFAALSPATPSE
                     SVDACVTAIYAETLLLSMDPAETRTWRRATAAWLTWELTGRFGCDEAVSAQQADPFRS
                     WIAGAAGDGAGGALWLAYLSARHDSVPGRFMRDVWGLASQRTWEGQELRAEPDLWSAI
                     ETAVNLSGDQLLDNVEALAVLRWFVGRSDLGDSVLAGIDDDAEVPIAREMKRLPTRVA
                     APRPLQSFGSAYIVMNASAWRGAPRLRAWLRGEYGVHWSFVAVQLDADGNEVRRLAAP
                     HTGSTPTAYVPIDLDETTQRLLFVVTNLADGLPDADEAEVHQRSFELILDRAED"
     gene            complement(2119..3477)
                     /locus_tag="VFG22_15870"
     CDS             complement(2119..3477)
                     /locus_tag="VFG22_15870"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HET6417775.1"
                     /translation="MTRVIDSDFGQREDDERGELGEEPPEPIVYAYAIPRRLVDQDAA
                     KVIRRLNQHGHTAYLVGGGVRDLMLGRKPKDFDLATSARPYEVRDLFRNCRVIGRRFR
                     LAHVLFSGGKIIEVATYRRDPSQTYEVIKPKIAKKIPLCAGPAPVRLIPSRRAITEVE
                     DADLLITNDNVFGEPHEDSIRRDFTINGLFYDLERGEVIDFVGGVADLEEHLLRTIGD
                     PNVRFREDPIRILRAIKFSARLDMGLDPEVYDAMVRHRGDLRRAAAPRVLEEILRFLR
                     GGAAHRSVFLSWDVGVLSEILPEVASFLDDDLEGASLTFGRLQALDALASEDRLPGDA
                     VLLAALLLGPIDEALDGVSDVPVAYEEFVRELSLRLSLPRRLKDRIRLLIMAQRRLRT
                     GRIQSIARREYFSDAATLFALDRMARGEGLPSWAQEPNDAVYESELPRPRRRRRRRRP
                     SA"
     gene            3578..4648
                     /gene="nagZ"
                     /locus_tag="VFG22_15875"
     CDS             3578..4648
                     /gene="nagZ"
                     /locus_tag="VFG22_15875"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HET6417776.1"
                     /translation="MSEIERMAGNVLVCGFDGMEVPSTLRRWLSTGTVAGLILFKRNI
                     ENVDQAVELIASCLEDADPELPQLISVDQEGGRVARFVDPVLRLPPMQKLASAGDPAL
                     TRDAAAVLGQQLRAIGINLDFAPVLDVNTNPLNPVIGDRAFGDTQEVVVEHALAFAAG
                     LHDGGVLSCGKHFPGHGDTDLDSHLALPILRHPLSRLRDVEFRPFRAAAADFPSLMTA
                     HVIFEAIDPNVPATMSPAAIEGLLRKELGFGGAVFSDDLEMKAITDRYSIEEAGVLAI
                     EAGCDLLLVCSDLEAAGRLRETLATEASHSRSFHTRLTEAVSRGYELRRRVLSLPPAL
                     PLRNALESAEACSVTERLQQIA"
     gene            4645..5637
                     /locus_tag="VFG22_15880"
     CDS             4645..5637
                     /locus_tag="VFG22_15880"
                     /inference="COORDINATES: protein motif:HMM:NF022427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HET6417777.1"
                     /translation="MTATPSLPAAPRSILVGEQWQIGVYRTPFEQAELEMLGLKRFRL
                     KEWHYVSFTTDEWFVAVGLVQLGYVANLFCYVFDRIQEGPAHEYGVLSPLGRALSFAP
                     SSLLGTTSWKNRNASVSITANEGWDLKLDIPVGAERLRGHAHIEAQDSLAMLHRLGPR
                     RVAYTHKAAGWPASGQLELGTRPIRLDTGLAASDWTRSQASRITKWKWASSSGYLRDG
                     TALGLNLSAQVYDDEKGHSVENALWLNGVVQRLSGVRFEMPSNPKTGRWAIRSIDGHE
                     VDMEFEPLGAREEHSNFGLVRTDFIQPYGRFSGSVAGHDITGCFGVVESHLSVW"
     gene            5650..7320
                     /gene="hflX"
                     /locus_tag="VFG22_15885"
     CDS             5650..7320
                     /gene="hflX"
                     /locus_tag="VFG22_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HET6417778.1"
                     /translation="MEIYGDTSGLSPSETTALQRIYRRRVPLERLTTPELTRALVGVS
                     HSTGRQVGVLVDRRGTVHNVILGDASKLMLPDVGRMRAGAGRLRGLRLIHTHLRNEPL
                     SRDDLVDLTRLHLDMVVAICLAPGEERALCLYYGHNFPVAEDTNEPPFRTFGPIPYAK
                     ARDNPAEIIETLEQEFARAARARGVTAKDGRAILIHVCDKRSAAQAEDSLRELRELAR
                     TAGVEVADTILQVRDRVDPRYALGRGKLEDVVIRAMQLDAETLIFDRELRPAQANNIA
                     SMTDLKVVDRTQLILDIFAQHAETNDGKLQVELAQMRYLLPRLGQRDDALSRLTGGIG
                     GRGPGETKLEVGRRRARERITRLQAQLKRLGRQREQRRSRRMRSGTPTVAIVGYTNAG
                     KSTLLNALTGSDVIAEDKLFATLDTRSRTMRLPGGQDVIMTDTVGFIRDLPKDLFAAF
                     RATFDETQDADLLLHVIDASDPCRREHVETTEKLLVDLGLERVPRILVYNKCDKLETP
                     QDTLLDRGVPISALDPGSLGALKRAIEDRIEVSHPMLQANEPTSSQPA"
     gene            complement(7298..8146)
                     /locus_tag="VFG22_15890"
     CDS             complement(7298..8146)
                     /locus_tag="VFG22_15890"
                     /inference="COORDINATES: protein motif:HMM:NF041621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5187 C-terminal domain-containing protein"
                     /protein_id="HET6417779.1"
                     /translation="MTPKEFEIMLSDAEVQLTRIKHLYEQWFQGLERIEPQIPRKQFD
                     RLLHELRKQQPRNTGLRFRFQTLIQRYTTLQTYWKRIGRQIEEGTYRRDLLRARRRRE
                     AAREQRALDRQRGSNSPVELDPNDDLDMDRLVADASDRMDQLLKAPDPSSKGATLDLD
                     DSTASAAASPATARFGKPKSRRPMPPVREQSISATMPSRRAPPPIAARSKGPGEARMR
                     QIYESYVEAKRNNRERTDKIDYETVAKSLKKMVPKLDRKHKGKRIDFQVVVKDGKVGI
                     KPVVKK"
     gene            complement(8157..8756)
                     /locus_tag="VFG22_15895"
     CDS             complement(8157..8756)
                     /locus_tag="VFG22_15895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417780.1"
                     /translation="MSCLLFGGCTPQGDPGIGTGFSDDFERTQLGKDYKSTGGNWRVE
                     RGQLHVHGAKNRPLWLLRVLPHDARVEFDARSDSAEGDIKVEVFGDGASYAEQDSYVA
                     TSYVVIFGGWSNSANVLARMDEHGEDRAVGRPTKVVPGKTYRFRIDRIDGTLTVWVDD
                     EVLVKMDDSEPLTGRGHDHFGFNNWKSSVWFDNLKVTPL"
     gene            complement(8812..9945)
                     /locus_tag="VFG22_15900"
     CDS             complement(8812..9945)
                     /locus_tag="VFG22_15900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417781.1"
                     /translation="MARFVAVFALLLAFIASASASAQSDNTIAQPINTDRFRPSLDRF
                     GFLGIQGSATPGHKRFNLGLMTWYANEPLRIRYTDGPQTGTAVELVDHRMVGDLFFEV
                     GLFGRWALALEIPLVFYQTGETDQPVNGRTSFTGFAVEDPRFTTKIRLVGKKTEETQN
                     RPDGPGLGFVLTMPVPIGSESFYVGEGQFTFDARLIGDFHLLGAGAGVMLGWQYRVNE
                     RTFGAATLGQEMRYGFALKPPIPPANDLFGLLELRGTTSFRGRSTNTLEGDIGVRWTK
                     SNVTMTAALGMGFIRGLGQPLFRALVGVAWSPQTDDLDADGIPDSRDECPRLPEDIDG
                     FEDADGCMDPDNDNDFIPDVDDRCPNDEAIEGQDLDEDGCTDR"
     gene            complement(9951..10739)
                     /gene="ttcA"
                     /locus_tag="VFG22_15905"
     CDS             complement(9951..10739)
                     /gene="ttcA"
                     /locus_tag="VFG22_15905"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007972.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HET6417782.1"
                     /translation="MNSNLAAPASDGYPSRVGHATLEKRLAHEMGRCIADYQLIEPGD
                     RVMVAISGGKDSYTLLHLLERARQRAPVRFEIIAVHLDQGQPGYDGAALEAWLEQQGY
                     AHRIVRKDTYRLVTDRIPEGQTYCSMCSRLRRGILYNVAEDLGCTKIALGHHRDDAIE
                     TLMLNMMFNGALSAMPARLRSDDGRNTVIRPLLYSSERDIAAYAEQLDFPIIPCNLCG
                     SQENLWRQQVKEMLDDLETRAPKVRQSMLAALKNVHTSHLLGTS"
     gene            complement(10736..12007)
                     /gene="hisD"
                     /locus_tag="VFG22_15910"
     CDS             complement(10736..12007)
                     /gene="hisD"
                     /locus_tag="VFG22_15910"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HET6417783.1"
                     /translation="MLTIYARGESGYDDTLASLSRRGDEDLARVEPDVRAILDAVRER
                     GDRAVLEYTERFDGRRPDSLVLKREDWLKAARTVDPAVREALTIAGERIRRYHEHQRD
                     PGFRYEEDGIELGQRVRPVAAAAVYAPGGKARYPSTVLMTAIPATVAGVERIVLVTPN
                     PTPEILAAAEVAGVTEVVDAGGAQAIAAVAYGTESVARVDKVVGPGNIYVACAKRLVF
                     GVVDIDSIAGPSEILVVADEGADPEIVAADLLSQAEHDEAAYPLAVVLSRTQADAVDA
                     ALQRQLASLPRRAIAGEAVRNNGYCFIVGDLQEAGRVATLLAAEHLSLSVADPEAMLG
                     QIGAAGAIFLGYHTPEAAGDYAAGPSHVLPTGGAVRFASPLGVYDFVVRSSLIRYSQA
                     AIDGQSDLLEGLARLEGLEAHARAVTLRRAK"
     gene            12091..13590
                     /gene="cysS"
                     /locus_tag="VFG22_15915"
     CDS             12091..13590
                     /gene="cysS"
                     /locus_tag="VFG22_15915"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HET6417784.1"
                     /translation="MNVRLYNTLTGQKEPFKPAEANHARIYVCGPTVYDYAHLGHARC
                     YVVYDVLVRHLRASGMGVTYVRNVTDVDDKILKRAAESGTEPTELATRFANAYSEDMH
                     RLGNLDPDVEPRVSTHLEDICNLVQRLVDGGHAYLSDGDVYFSVESFPEYGKLSHRDL
                     GQMKLGASDRLDERQTARKRHPADFALWKKSEKDAWGWDSPWGRGRPGWHIECSAMSM
                     KHLGDTLDLHGGGLDLVFPHHENEIAQSEAATGKQFARCWMHNGFVEVDKEKMSKSLG
                     NFFTARDLFDRVEPEAIRYFMMTVHYRAPLNLDWTLDDAGNVTGFPQFEDAERRLAYL
                     YKTKQRLESLPPNRVVDQDGATAPEIAGFSKALRESLDDDLNMPIALAKLADFLKAVN
                     ELGDRAMRKKGKASRAAVEQAGAGFRALEAELGIGAEPADQILTRIRDRRAKARGLDA
                     SVIEGRIADRTAARKSKDFGEADRIRDELAAQGVELLDSAEGTGWTISD"
     gene            13717..15360
                     /locus_tag="VFG22_15920"
     CDS             13717..15360
                     /locus_tag="VFG22_15920"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HET6417785.1"
                     /translation="MTSSVPKPLAEGTLEATPFGHVLLSIQKKGLSGTLAVWPEEERS
                     GQDRILFRSGVPAVGRFLDPAADLERGILNIFHRQNAPYAFYEADLVGDSAGALRGQT
                     DILALIATSLRADVPKDAMVRVIGKLGLERQRVKTGAPLPRLGLQSNEMAFVELMRAE
                     PATAAILIEQFGDPKVAQRMLYLLAITECLEPYRPVSQQKLRAVSPPPQGAQRSSTPP
                     AARISVTPPASSGSAFPSRGRSSSPPGLLDSMLPGGDSVLPKSLSNLPPAKTLSGEIV
                     APRSLPPEPPVDVDPELKSRWEEVARVISMMDRQNYFQMLGAKESEDSSGIRTKYMTL
                     AKKWHPDRLPPDLAALRPWVEEIFHLLTVANDTLSDTKKRAEYQRAVMQGGGTPESER
                     KLNVVVEAAINFQKVDILVKRKRYDDALKICDAAMAVVRKEADYPAMKAWILMLRDGV
                     DDDEVVDEIRGLLRTTFAINPDHVHGHYVRAHFLKRRGQHDKALKHFKKVAKLDPKNL
                     EAAREVRVGTMRQSRGRSSAPPPRGGGKRPSLFGKFFKK"
     gene            complement(15429..16355)
                     /locus_tag="VFG22_15925"
     CDS             complement(15429..16355)
                     /locus_tag="VFG22_15925"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6417786.1"
                     /translation="MKTRIAALIIAGLVTSLAWTFVKRPLPVPKTMGPRLPPSVEVDS
                     VTFGVIRGAEMKSQEGFSVRGGSLTTPFTSGLVAFVVEHPRGRLLIDAGVGRDVSEHL
                     KTTPLLLRTLASLTVQRPTIDALAARDLAPSDLRGIVLTHSHWDHVSGLADLREVPVW
                     IAPEELAHARSEDEGGKLYRQLEAEGPFRLEVLTYPSGAYGPFSASHDFFQDGSVVLV
                     PMPGHTPGSIGVFVSLPNGQRFLIVGDTSWTKEGVDWPAEKPWLARRMVDHDPAGVRE
                     QLVLLHQLQRANPELIIVPAHDARVHETLESF"
     gene            16442..17206
                     /gene="radC"
                     /locus_tag="VFG22_15930"
     CDS             16442..17206
                     /gene="radC"
                     /locus_tag="VFG22_15930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HET6417787.1"
                     /translation="MGLGEAPGIWVSWTGTGAALDCGMNGPRERLAHDGVKSLSDADL
                     LAVLLGTGPAGEDVITLASRVLKEAGGLWALARMGATELARIRGIGPAKAARLAAAFE
                     AGTRGLVSPDTSTQPLSNSELVFLRYGRPLTTSAIERFVVISVDAKNRARAAREVARG
                     GRVSCQVDPAEVFRLLVSESASGAIFLHNHPSGDPEPSPQDLELTERLVAGGSLLGIR
                     ILDHLIIGGGRYRSLRDLDLWPSNLAKSSSSARRRS"
     gene            17276..17425
                     /gene="rpmH"
                     /locus_tag="VFG22_15935"
     CDS             17276..17425
                     /gene="rpmH"
                     /locus_tag="VFG22_15935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HET6417788.1"
                     /translation="MKRTYQPHRKRRLRTHGFRARMKTRGGRSVINARRRKGRTRLTV
                     SCYKK"
     gene            17443..17835
                     /gene="rnpA"
                     /locus_tag="VFG22_15940"
     CDS             17443..17835
                     /gene="rnpA"
                     /locus_tag="VFG22_15940"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HET6417789.1"
                     /translation="MPQTSETTGASSDEPQRFRRGDRLKKRYEFRQAQLRGRRIHTPH
                     FLIVVLPNALQNTRLGITVTKKVGTAVERNRIKRVVREVFRRNRTLFPAGHDLVFIAK
                     RGSTDIAYDSLLSEVERAAKKLQPEAAR"
     gene            17832..18089
                     /gene="yidD"
                     /locus_tag="VFG22_15945"
     CDS             17832..18089
                     /gene="yidD"
                     /locus_tag="VFG22_15945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HET6417790.1"
                     /translation="MMRAALLFLIRLYQMTLARLIGPSCRFEPSCSRYTATCIERFGA
                     LRGSWLGIKRIGRCHPLNPGGFDPPPNVKHTHDHPREAQHG"
     gene            18082..19863
                     /gene="yidC"
                     /locus_tag="VFG22_15950"
     CDS             18082..19863
                     /gene="yidC"
                     /locus_tag="VFG22_15950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET6417791.1"
                     /translation="MDDRKQPPIALLMLPLVALGAMLAVQYFAPPKAETEEPPTELTA
                     PVQAEQPSGETAPAQIAPSDSRSRTEAQTLYTLENDRFAATFTNLNTAMVSLRVKGER
                     YVDKSGEPFELVTTDKEAYLPLAPSFTGVTLPPDARWRIAESSGSKLVFQWAGDGVSI
                     TRTWELAEPPYQLHSSIEVRNDDPNTRSIGLGITSAHYVRREDEKGGFMARPSPALSH
                     GICQQGDETIREDRDGLLSEDETRPLQPHSYGPGVEYVALTSVYFAIVAAAEGEPAER
                     CDMRTTERGGTVDDPDGSLFEARLAYAPREIGPGETAAFRTAVYAGPKDYDALEGFGH
                     GASNIIDLGWFSFIAHGMSWLLRTIYGFIGNWGVAIILLTLLVRVLLLPFVIPQFRNM
                     AKQRALKPEIDKINELYKDDREKKGAAMMELWRKHKVNPLGGCLPVLLQMPIFFALYQ
                     TLSTSIELYHAPFVLWWTDLSSPDPFYVLPVVLGALMFIQQKLTPVQMDATQAKVMLY
                     AMPLMMTFFMLLLPTGLCLYMVTSSAVGITQQRYMYWKMDQEASALSASSDDPEEDDN
                     QAAEGSKAAAPKQAKPRRKKRTRGGRA"
     gene            19850..20392
                     /locus_tag="VFG22_15955"
     CDS             19850..20392
                     /locus_tag="VFG22_15955"
                     /inference="COORDINATES: protein motif:HMM:NF024483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HET6417792.1"
                     /translation="MDELKGRPRDGALILDQPTQEDPLQYAGDGKAEEAAEILEELMN
                     LMDLDVRVDIRSDGERIELDVTGEDSGRVIGKKGATLDALQFLINKMVNRFPEGRRHV
                     IVDSGDYRERHDDGLVSMAKRQAERAVAEGKVVTLQPMNARDRRVIHMSLAKFDGVST
                     MSQGEGTRRRIQIIPARRRR"
     gene            complement(20408..21022)
                     /locus_tag="VFG22_15960"
     CDS             complement(20408..21022)
                     /locus_tag="VFG22_15960"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmG family class I SAM-dependent
                     methyltransferase"
                     /protein_id="HET6417793.1"
                     /translation="MSGSRAIGKVCDAAGVSEPGGWDAYFDLVKAWSARTDLTSARSD
                     LELAEILFLDAAQLIRAAWPTSSASIVDIGAGVGAPSIPLMLSDASLHGTLVEPRRIR
                     VAFLRTAVGTLGLAERVTTLEIKVDPTEPKVEGTPFTVALSRATFSPEQWLTIGARLA
                     DEVWVLTAGAEPAPIGDLRLTRRLDYEVPSSSAPRTILAYAHRD"
     gene            complement(21019..21858)
                     /locus_tag="VFG22_15965"
     CDS             complement(21019..21858)
                     /locus_tag="VFG22_15965"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6417794.1"
                     /translation="MKDTKTIGLGTVLAGRYRLVAEIARGAMGNVFEATDQLDGSKAA
                     VKVLRAEYLADESMRRRFRREGAILKAIDHPAIVQLLELGVDEDGQVFLVTEYVEGET
                     LATRLLHGPLTITAADALVDALCGGLGAVHDHGVLHGDLKPANVIWHEDGDPRLVDFG
                     ASKILGLDRLTATGELSGTPAYMAPEVLTGKASVDQRIDVYGLGVVLYEALSTERPFR
                     DSHLGRLVMKIDAGDCLPLDAVAEVPARIAEVVGRTMHHNKDERIASVAEVKAAWQEA
                     RRP"
     gene            21922..22989
                     /locus_tag="VFG22_15970"
     CDS             21922..22989
                     /locus_tag="VFG22_15970"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET6417795.1"
                     /translation="MSLPNLHRPLAKLYGEPLPSFRIVKLRGDASTRSYYRVYAEGSA
                     GGFPESVIVMRLPDDALGSDEGGPQHSTHRLPFVEVAELLRSKGLPVPGIFVEDLENG
                     ILLLEDLGDTTFEQGLRGVPSSDWEGLYARAIDLLADLHERCADLPKDSIVSRRRFDR
                     GLLEWELEHFREWGLEALWGPLDGLQSRTLSRAFSDIVLEIEAMPYGFVHRDYQSKNL
                     MLGPGGVLTLIDFQDALLGPRAYDLVALLCDSYVSIGLELQESMIEHYAALRQIQVAT
                     LRREFWLVALHRKLKDAGRFIFIDRVRKNPDFLQWYPQSMEYVGRAAAQAQGFEPLAA
                     VLGLKIRGFPDEVQKPSSSVE"
     gene            23138..23524
                     /locus_tag="VFG22_15975"
     CDS             23138..23524
                     /locus_tag="VFG22_15975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417796.1"
                     /translation="MSGVDFRFAFMRTLFQALALAAILFLDAASADAADCICDQLSTE
                     GCTQSAQVRDEPIAPPLWCERTDDPRCMPASTHGTSTSTLVPVAMSWAQPIRWGAPPR
                     TGIQMAVRVEGKARTEHSRRVERPPR"
     gene            23632..25629
                     /locus_tag="VFG22_15980"
     CDS             23632..25629
                     /locus_tag="VFG22_15980"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET6417797.1"
                     /translation="MSKGAAILLAIVIAIGAFLVGRLVSSPGDTDNGTAEAGGEEAKA
                     ADVAIADDVERYKVPVTDAQPSKGPKDAPVTIVEFSEFQCPFCARVLPTMKEVVDTYG
                     DKVRVVWRNNPLAFHNNAGPAAAVAMEAYAQGGSEKFWEVHDVLFANQKALDRAQLEE
                     YAQKAGLDMDKVKKAIDEGTHSKAIGDDQTLASQLGANGTPYFFINGRQLRGAMPFTA
                     FKKVIDEELATAEALTKQGVKPEQMYATLTKDGLTKAAAAKPTPQGAQPGQPDPSAVY
                     KVPLKGDEPQKGPDDALVTIVEISDFECPFCGRVEPTLKEVLDKYGDDVRVVWLNNPL
                     PFHRNAKGAANAALEAHAQKGNAGFWAMHEKMFENQKALTTDNLEKWAKELGLNMAQF
                     KKALADDKYGKKIEEQQALASSLGARGTPAFFINGRNLRGAQPLTAFTALVDEELVKA
                     KALVAKGTPKAKVYATIIANGETGPKTAPAAAQPDANKVYDIPVPKKAPTKGAKNAKV
                     IIQEFSDFQCPFCGRVNPTIAQLMTEYGDKVKIVWRDYPLPFHQDAQPAAQAAREVFA
                     QKGDKAFWAYHDLLFQNQKTLTRENLEKFAEQVGGIDMKAFKAALDSGKHKAAVDADI
                     AAVSKAGARIGTPSFFINGKLLQGAQPYPAFKAAVDEALKN"
     gene            25758..26663
                     /locus_tag="VFG22_15985"
     CDS             25758..26663
                     /locus_tag="VFG22_15985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417798.1"
                     /translation="MVRAKAAAAKRVSKKPIVEELYFRSDSAYLCLLLNRRTRSIRVI
                     DFRAGALPAKRLYIQSVATQENVEKVITLVEKDEVSSWTRVGFVREGTIPGFYKRSDG
                     HLCGCVIGDKTASIEVSDASAKLTERTINAAKKAASDIPEKIKGASVRSASEKDALAA
                     RDAAWRKQPAFGSFDMFGRDAQRIYFDMSLRRSKTNYLSAEFQDCFGHALVEVLRLPT
                     NENETLAVTAALRTLGDNLQERGIVSTFAFAPNDNVEISTAFVAAGYRKTGLLAQGIL
                     DADGGRKDAILWTLKFAGALGEEFE"
     gene            26720..27112
                     /locus_tag="VFG22_15990"
     CDS             26720..27112
                     /locus_tag="VFG22_15990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417799.1"
                     /translation="MRSREDIESYLVRADVSYEEVGDDGMWLVHHGSLSENIAIIAAG
                     PLLLFRVKVLELREIEDKASLYERLLSLNASDLVHGAYGISDDSVVLTCALQLENLDY
                     NELQGVLDDFSLALANHYEQLTKFRIAT"
     gene            27125..27913
                     /locus_tag="VFG22_15995"
     CDS             27125..27913
                     /locus_tag="VFG22_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HET6417800.1"
                     /translation="MGFLSRLAQLIKSNINDLINKSEDPEKMLNQVIVDMNQQLVEAK
                     KQVAIAIADEKRLYKQLGNEAKKSEDWEKKAMLAVRSGDDELAKEALLRRKEHDAIAL
                     QYQEQWERQKALTDQLKTALKALNHKIGEAQRKKNVLIARKRRAEAMKNIQETMSGLS
                     DASAFETFDRMAEHIEQMEAEAEAGAEVAEEHTGDILKHRFSELEATAGADLELEALK
                     RKMGLLPSEPPVVSARIASPGEAYEEEELAELEEALEELKRRER"
BASE COUNT         5646 a         8785 c         8641 g         5041 t
ORIGIN      
        1 ccggcgagga ggattgggcc cccggccgca tcggtggtga ggtctgcgcg gccgccgccg
       61 aagtcgtacc cgaagccgtg cgcattcgct cgagcgcttc tgcagcgtga gtcgttcgct
      121 cctcgggccg cttcgctcga actttgttga gcagggaccg aagtctgtct tcgcggtcgt
      181 tcatgatgcc ctcttcgtct cgagcatgcc tacaatcgcc ggcaaatctg ctccgtcctt
      241 gaaccatccc caaacggttt cggccacttc gaaatctacc atgatgaatt tcgagtcgcc
      301 cgtcggcaca caggaaacct gccgttgaga gatttcggag aacattccac cgagcaaggc
      361 tgcggcgccg agctcgtctt cgtcgagctc gggcttgtcg cgcagcacga tcaccaatgc
      421 agaaccccag cgctcgaagg tgaggcggcc ccagccaaac agcgaggtca ccgcggatgc
      481 gtggccgaga accgcttccg gcgaaaggac cgaagcgggc cggtccaaac caccgatcac
      541 ctgctcacca agcaactctc ccaatcgccg aagcggggtg agatcaccat cgcgagctgc
      601 ggctgcaacc aaagaagaga gcacatcctc gctgacgatg gccacgcgct cgccggtgcg
      661 agtgcgaacc atgcccgcat tcagattgaa ctcgtagaag ccgctaggat cgaaacggcc
      721 gtcgtgcata ggtccccctc cagtagggat gtagaaggaa taggcggatc gtgtcaaatg
      781 ccacgggtga aatcaaatct cgcttgcgtc gtactggcga tcagcttgca ggcgcaagcc
      841 gcacgggcgc aaggcgattt gctgggcgcc gacgagatgg ccgaggcgat cgtgtcgatg
      901 acctcgccgg tctggacggg cccccgaacg ccgcccccgg atcgggttgg gcgacccgct
      961 agggcttccc aggttttgca ctccacgaat tccctactgg cggttcacgc cgacccggag
     1021 gtggacccgc tcgtggtgcg gcgggcgttg agagcgctcg agtatgcaag agggcgcctc
     1081 gacgcgatgg gttggccgtc gccgatctcg gacggcaacc tcggaggcgg acccgagctc
     1141 gatctctact tgagcggcga gctccccccg gacgcgtact tcgacggctt ggcgcgctgg
     1201 agctatctgg accggggctc ggcctttgct gcgctcagtc ccgcgacacc gagcgagagc
     1261 gtcgacgcgt gtgtaacggc gatttacgcg gagacgctct tgctgagcat ggaccccgcc
     1321 gagaccagga cgtggcgccg tgccactgcg gcctggctga cctgggagct caccgggcgc
     1381 tttggctgtg acgaagccgt gtcggcccag caagcggatc cgtttcgctc gtggatcgct
     1441 ggagctgccg gcgatggcgc gggcggcgcg ctttggctcg catacctatc cgctcggcat
     1501 gacagcgtac cgggtcgctt catgcgggac gtctgggggc tcgcgagtca gcgcacctgg
     1561 gaggggcagg agcttcgcgc tgaaccagac ctgtggtctg cgatcgaaac cgcggtcaat
     1621 ctgagcggcg accagctgtt agacaatgtg gaagcgctcg cagtgcttcg ctggttcgtg
     1681 gggcggtccg acttgggcga ttcggtgctc gctggaatcg acgacgatgc tgaggtcccg
     1741 atcgctcgcg aaatgaagcg cctcccgact cgggtagcgg cgccgaggcc gctccaatca
     1801 ttcggaagcg cgtatatcgt catgaatgcc tctgcatggc gcggagcacc ccgtctgcgc
     1861 gcgtggcttc gaggcgaata cggtgtccac tggtcgttcg tcgcagttca gctggacgcc
     1921 gatggaaacg aggttcgccg actcgcggct ccgcatacag gctcgacgcc cactgcatat
     1981 gtcccaatcg acctggacga gacgacccaa cgtctattgt tcgtcgtgac gaatctggcc
     2041 gatggattgc ctgatgcgga tgaggccgag gtacatcagc gttcgttcga gctgatcctg
     2101 gaccgcgccg aggactgact aagcgctcgg gcggcggcgg cggcggcgac gccgtgggcg
     2161 cggcagctcg gactcgtata ccgcatcgtt cggctcctgt gcccaggaag ggagtccttc
     2221 ccctcttgcc atacgatcga gcgcaaacaa ggtcgcggca tcggagaagt actcgcggcg
     2281 cgcgatcgat tggatgcgtc ccgtgcgaag acggcgctgc gccatgatca gcaaccgaat
     2341 gcggtctttg agacgccgcg gcaatgaaag tcgaagagaa agctcgcgca caaactcctc
     2401 gtaagccacc ggaacgtccg atacgccgtc gagcgcctcg tcgattggtc cgagtaagag
     2461 tgccgcgagc aagacagcgt cgccgggcaa gcgatcttcg gaggccaggg cgtcgagcgc
     2521 ctgcaagcgc ccgaacgtga gcgatgcacc ttcgaggtcg tcgtccagga acgaagccac
     2581 ctcaggcaga atctcgctga gaacccccac gtcccaggag agaaagaccg aacggtgtgc
     2641 cgcaccccca cggagaaagc gcagaatctc ttcgagcacc cgcggcgccg cggcacgccg
     2701 gaggtcacca cggtgccgca ccatggcgtc gtaaacctcc gggtcgagcc ccatgtcgag
     2761 gcgagcgctg aacttgatcg ctcgaaggat gcggatcggg tcttctcgga agcggacgtt
     2821 cgggtcgccg atcgtgcgaa gcaagtgctc ttcgagatcc gcaacgccac ccacgaagtc
     2881 gatcacctcg ccacgctcga ggtcgtagaa caaaccattg atcgtgaagt cgcgtcgaat
     2941 ggagtcctcg tgcggctctc cgaatacgtt gtcgttcgtg atcagaaggt cggcgtcttc
     3001 tacctcggtg atcgcccgtc gcgatgggat caagcgaact ggcgcaggac cggcgcaaag
     3061 gggaatcttc ttggcgatct tgggtttgat cacctcgtag gtctgggagg gatcgcggcg
     3121 gtacgtcgct acctcgatga tcttgccacc agagaacagg acgtgcgcca atcggaagcg
     3181 acggccgatg acgcggcagt tgcgaaacag atcacggacc tcgtaaggcc gcgcgctcgt
     3241 ggcgagatcg aagtcctttg gcttccgtcc cagcatcaag tcacgcacac cccctcctac
     3301 gaggtaggcc gtgtggccat gttgattgag gcggcgaatc actttcgcgg catcttggtc
     3361 aacgagccta cgcgggatcg cgtaggcgta gacgatcggc tcaggcggct cttctccgag
     3421 ctcgccacgc tcatcgtctt cacgctgacc gaagtcagaa tctataactc gggtcatcaa
     3481 agtccaaagc ccgcacaggg caagaagtaa ttaccagctt gtagcaacaa cgggccctga
     3541 ccgcaatcta agaccaatgc tacggtctgc gaacaatatg agcgaaatag aacgaatggc
     3601 gggcaacgtg ctcgtctgtg gcttcgatgg aatggaagtg ccctccacgc tgaggcgatg
     3661 gctatcgacg ggaaccgtag ctggtttgat cctcttcaag cgcaacatcg agaacgtcga
     3721 tcaggcggtc gagctgatcg cttcctgctt ggaagacgcc gatcccgagc tcccgcagct
     3781 catctccgtc gatcaggaag gcgggcgagt agctcgtttc gttgatcccg tcctgcgact
     3841 tcctcccatg caaaagctgg cgtcggcggg tgatccggcg ctgacgcgcg atgcagcagc
     3901 cgtcctcggg caacagctgc gcgcgatcgg aatcaacctc gacttcgcac ccgttctcga
     3961 tgtgaacacc aacccgctca acccggtgat tggcgaccga gcgttcggcg acacccagga
     4021 agtcgtcgtt gagcacgccc tcgcgttcgc cgctggcctc cacgatggcg gagtcctctc
     4081 ctgcggcaag cacttcccgg gtcacgggga caccgacctc gacagccacc ttgcccttcc
     4141 gatcctccgt catccgctct cccgtctacg ggacgtggag tttcgtccct ttcgcgcagc
     4201 cgccgccgat ttcccatcgc tcatgaccgc acacgtaatc tttgaagcca tcgacccaaa
     4261 tgtccccgcg accatgtctc ccgcggcgat cgaagggctt ctccgaaaag agctcggatt
     4321 tgggggcgcg gttttctccg atgatctaga gatgaaagcg attacggacc ggtactcgat
     4381 cgaggaggct ggcgtactgg cgatcgaagc gggttgtgat cttctactcg tctgctcgga
     4441 cctcgaggcc gctggcaggc ttcgagaaac tctggcgacc gaggcgagcc acagcaggtc
     4501 gttccacaca cgactgaccg aagccgtgtc acgcggatac gagctccgaa ggcgagtcct
     4561 cagcctgccc cctgcccttc cgctgcgaaa cgcgctagag agcgccgaag cgtgctccgt
     4621 cacggaacgc cttcagcaaa tcgcatgacc gcaaccccgt cacttcccgc cgcgccacgc
     4681 tcgatcctcg tcggcgagca atggcaaatc ggcgtctacc gaaccccctt cgagcaagca
     4741 gagctcgaga tgctgggctt gaagcgcttt cgcctcaagg agtggcacta cgtttcgttt
     4801 accacggatg aatggttcgt tgccgtcggt ctggtgcagc tgggctacgt ggccaacctg
     4861 ttttgctacg tgttcgatcg gattcaggaa ggccctgcac acgagtacgg agtcttgtcg
     4921 ccgctcggcc gcgcgctgag cttcgccccc agctcgctgc tcggaacgac ctcatggaag
     4981 aaccgaaacg cgagcgtctc catcaccgcc aacgagggct gggacctgaa gctcgacatc
     5041 ccagtcggcg ccgagcggct gagagggcac gctcatatcg aggcacaaga ttcacttgca
     5101 atgcttcacc gactcggtcc gcggcgggtg gcctacaccc acaaggccgc aggctggccc
     5161 gcctcggggc agctcgagct cgggacccgc ccgattcgcc tcgacacagg gctcgccgcc
     5221 agtgactgga cccgttcgca agcgagccgc atcaccaaat ggaagtgggc ctcctcgagc
     5281 gggtacttgc gcgacggcac cgcgctaggc ttgaacctct cggctcaggt ctatgacgat
     5341 gaaaaggggc attcggtcga gaatgcgctc tggctgaacg gagtcgttca acgcttgtct
     5401 ggcgtccgct tcgaaatgcc gtcgaacccc aagacgggac gatgggcgat tcgaagtatc
     5461 gatggccatg aggtcgacat ggagttcgag cccctcggcg ctcgcgagga acacagcaac
     5521 ttcggcctgg tgcgaaccga cttcattcaa ccctacggtc gattctcggg cagcgtcgcg
     5581 ggacacgaca tcaccggttg cttcggcgtc gtcgaatcac acctttcggt ctggtagcct
     5641 gaggtagctt tggagattta cggcgacact agcggcctca gcccctcgga aaccacggcg
     5701 ctgcaacgga tctatcggcg gcgcgtgcct ctggagcgcc tcacgacgcc cgagctcaca
     5761 cgcgcgctcg tgggcgtttc gcactcgacg ggaagacagg ttggcgtcct cgtcgaccgc
     5821 cgtggcaccg tgcacaacgt gatcttgggc gacgcctcca agctcatgct gccggacgtg
     5881 ggccgcatgc gcgccggggc gggcaggttg cgcgggcttc gactgatcca tacgcatctg
     5941 cggaacgagc cgctcagccg cgatgacctc gtcgacctca cgcgcctgca tctcgacatg
     6001 gtcgtcgcga tctgtctcgc tcccggcgag gagcgtgcgc tgtgtttgta ctacggccac
     6061 aatttccccg tcgccgagga tacgaacgag cccccgtttc gcaccttcgg tccgattccg
     6121 tatgcgaagg cacgcgacaa tccggctgag atcatcgaaa ctctggaaca ggagttcgcc
     6181 agagccgcgc gcgctcgtgg agtcaccgcc aaagacggcc gcgcgatttt gatccacgtc
     6241 tgcgacaagc gaagcgccgc tcaggcggag gactcattga gggagctgcg ggagctcgcg
     6301 cgcactgccg gtgtcgaggt ggcggacacc atcttgcagg ttcgcgaccg cgtcgaccct
     6361 cgatatgcgc taggccgagg gaaactcgag gatgtagtaa tccgcgcgat gcagctcgac
     6421 gccgaaacac tcattttcga tcgagagctg cgccccgctc aggcaaacaa catcgcgagc
     6481 atgactgact tgaaggtcgt cgatcgcacc cagctcattc tcgatatctt cgcgcagcat
     6541 gccgaaacca acgatggcaa gctccaagtg gagctcgcgc agatgcgcta ccttctgcca
     6601 cgtctcggtc aacgcgacga cgcactctca cgtctcaccg gcggcatcgg agggcgaggg
     6661 cctggcgaga ccaagctcga agtcggccgg aggcgcgcca gggagcgaat cacccggctc
     6721 caagcacagc tgaagcggct gggtcggcaa cgagagcaac gacgctcccg tcgaatgcgg
     6781 agtggcactc cgacggttgc gatcgtcgga tacaccaacg cggggaaaag cacgctcctc
     6841 aatgcattga ccggaagcga tgtgatcgca gaggacaagc tcttcgccac cctggatacg
     6901 cgctctcgaa ccatgagact tcccggcggc caggacgtga tcatgacaga taccgtcggg
     6961 ttcattcgtg accttccgaa ggatctcttc gccgcattca gggcaacttt cgacgagacg
     7021 caagacgccg acctcctgct ccacgtgatc gacgcgtctg atccctgccg ccgggagcac
     7081 gtcgaaacca cggaaaagct gcttgtcgat ctgggtctcg agcgagtccc cagaattctc
     7141 gtgtacaaca aatgcgacaa gttggagaca ccgcaagaca ccttgttaga tcgcggtgtt
     7201 ccaatcagcg cgctcgaccc aggctcgctc ggggcgctca aacgcgcaat cgaagaccgc
     7261 atcgaggtct cgcacccaat gcttcaggca aacgaaccta cttcttcaca accggcttga
     7321 ttccgacctt gccgtccttg acgacgactt gaaagtcgat gcgcttgccc ttgtgcttgc
     7381 ggtcgagctt gggaaccatc ttcttgaggc tcttggcgac ggtttcgtag tcgatcttgt
     7441 cggtgcgctc gcggttgttc cgcttggctt cgacatagct ttcgtagatc tggcgcatgc
     7501 gcgcttcgcc cggacctttt gagcgcgccg cgattggcgg cggcgcgcgg cgactcggca
     7561 tggtcgcaga aatcgactgc tcgcgcaccg gtggcatcgg cctgcgagac ttgggctttc
     7621 cgaaacgtgc cgttgccgga cttgccgctg cagaggcggt cgagtcatcg agatcgagtg
     7681 tcgccccttt cgacgatggg tcgggcgcct tcaagagctg gtccattcgg tcgctcgcgt
     7741 cggcaaccag tcgatccatg tcgagatcat cgtttggatc gagctcgacc ggactattgc
     7801 tgccgcgctg ccgatccagt gcgcgttgtt ctcgcgcggc ctctcgcctt cgtctcgccc
     7861 ggagcaagtc gcgacggtac gtgccctctt cgatctggcg cccgatgcgt ttccagtagg
     7921 tttgcagcgt ggtgtaacgc tggatcaagg tttgaaaacg aaagcggagc ccggtgtttc
     7981 tgggctgctg ctttcgcaac tcgtgaagca ggcgatcgaa ctgcttgcgg ggaatctgcg
     8041 gctcgatgcg ctcgaggccc tgaaaccatt gctcgtatag atgcttgatg cgcgtgagct
     8101 gcacctcggc atccgacaac atgatttcga attctttcgg cgtcattacc caagcatcag
     8161 agcggtgtga ctttcaaatt gtcgaaccag acggaagatt tccagttatt gaagccgaaa
     8221 tggtcgtgtc ccctgcccgt gaggggctca gaatcgtcca ttttgacgag aacctcgtcg
     8281 tccacccaca ccgtcagagt gccatcgatc cggtcaatcc gaaaccggta ggtcttgccg
     8341 ggcaccactt tggtggggcg tccgacggca cggtcctcgc cgtgctcgtc cattcgggcc
     8401 aagacgttcg cagagttgct ccatccgccg aagatgacga cgtagctcgt ggcgacatag
     8461 ctgtcctgct ctgcgtagga tgctccgtct ccgaaaacct cgaccttgat gtcgccctcg
     8521 gcgctatcgc ttcgcgcatc aaattcgacc cgtgcgtcgt gcggcaaaac tcgaagcagc
     8581 cagagcggcc ggttcttcgc gccatggacg tgaagttgcc cacgctcgac acgccaattc
     8641 cccccggtgc tcttgtagtc cttgccgagt tgcgtgcgct cgaagtcgtc cgagaatccc
     8701 gtcccaattc cgggatcccc ctgcggggta cagccgccga aaagcagaca agacaacact
     8761 gccaccgtcg ctgacactga cgctgacgct gacgctgaag atgtccccag cttaccgatc
     8821 ggtgcacccg tcttcatcca agtcctggcc ttcgattgcc tcgtcgtttg ggcacctgtc
     8881 gtcgacgtcg ggaatgaagt cgttgtcgtt gtcgggatcc atgcagccgt ctgcgtcctc
     8941 aaacccgtcg atatcttcgg gaaggcgcgg acattcatct ctggaatccg gaatcccatc
     9001 ggcgtcgagg tcgtcggtct ggggagacca ggcgactccg acgagcgcgc gaaacaacgg
     9061 ttgaccgaga ccgcggatga agcccattcc gagcgctgcc gtcatcgtca cgttgctctt
     9121 ggtccagcga acgccaatgt ccccttcgag cgtgttggtg ctgcgcccgc gaaacgatgt
     9181 ggtgccacga agctccagaa gcccaaacaa atcgtttgcc ggggggatcg gtggcttcaa
     9241 ggcgaagccg taccgcatct cctgacctag cgtggcagcg ccgaacgtcc tttcgttcac
     9301 gcggtattgc catcccagca tcacgccggc gcctgcgccg agaagatgaa agtcaccgat
     9361 cagcctcgca tcaaacgtga actggccttc gcccacgtag aagctctcgc ttccgatggg
     9421 cactggcatc gtgagcacga aacccaagcc aggaccgtcg ggacgattct gggtctcctc
     9481 ggtcttcttg ccgaccagcc tgatcttggt ggtgaagcga gggtcttcga cggcgaatcc
     9541 ggtgaacgag gttcggccgt tgaccggttg gtccgtttcg ccggtttgat aaaacaccag
     9601 tggtatctcg agggcgagag cccacctgcc gaagaggcct acctcgaaga acaagtcgcc
     9661 aaccatgcgg tgatcgacga gctcgacggc cgtccccgtc tggggtccgt cggtgtaccg
     9721 aatccgcaac ggctcgtttg cgtaccaggt catcagaccg agattgaagc gtttgtggcc
     9781 cggggtcgcg cttccctgga tgccgagaaa accgaagcgg tcgagcgacg gccgaaagcg
     9841 atcggtgttg atcggctgcg cgatcgtgtt gtccgactgt gcggacgccg atgccgaggc
     9901 gatgaacgct agaaggagcg cgaagacagc gacgaaacgc gccatctgcg tcaactcgtt
     9961 ccaagcagat gagaagtgtg aacgttcttg agcgccgcaa gcatgctctg ccgcaccttg
    10021 ggggcccgcg tttcgaggtc gtcgagcatc tccttgacct gttgacgcca gaggttctcc
    10081 tgggatccgc acagattgca cgggataatt gggaaatcca actgctcggc gtacgccgcg
    10141 atgtctcgct ccgacgaata gagcaagggc cgaatcaccg tattacgccc gtcgtcactg
    10201 cggagcctcg caggcattgc gctgagagcg ccattgaaca tcatgttgag catgagggtt
    10261 tcgatcgcat cgtcccgatg atgtccgagg gcgatcttcg tgcagcctag gtcttcggcc
    10321 acgttgtaca aaattccgcg acgaaggcgt gagcacatgc tgcaataggt ctgcccctcc
    10381 gggatacgat ccgtcaccag tcgataggtg tccttgcgca cgatccgatg cgcgtatcct
    10441 tgctgctcta gccaggcctc cagtgccgcg ccgtcgtacc ctggttgccc ttggtcgaga
    10501 tggacggcga tgatctcgaa ccgcaccggc gctctttgcc gagcccgttc caaaaggtgg
    10561 agcaaggtgt aactatcctt gcctccgctg atcgccacca tgacccgatc accaggctcg
    10621 atgagctgat agtccgcgat acagcggccc atctcgtgcg cgagacgctt ttccaaagtg
    10681 gcatgcccca cgcgggatgg gtacccgtcg gacgccggcg ccgcaaggtt cgagttcact
    10741 ttgctcttcg tagtgtgacg gcgcgcgcgt gcgcttccaa gccctcgagc cgggcgagcc
    10801 cttccaggag atccgactgg ccgtcgatgg ctgcctgcga gtagcgaatc agtgacgagc
    10861 ggacgacgaa atcataaaca ccgagcggag aggcgaagcg aaccgccccc cctgtcggca
    10921 agacgtggct cggaccggca gcgtagtccc ctgccgcctc aggcgtatga tagccgagga
    10981 agatggcgcc agcggcccca atctggccaa gcatggcctc gggatccgcg acggacaggc
    11041 tgaggtgctc tgccgcgagc aacgtcgcga cgcggccggc ctcttggagg tcgccgacga
    11101 tgaaacagta cccattgttg cgcacggcct caccggcgat ggctctccga ggcagtgagg
    11161 cgagctgccg ttggagcgcg gcatcgactg cgtcggcctg cgtgcgtgaa agaacgaccg
    11221 cgagcgggta cgcagcctcg tcgtgctctg cctgagacag aagatcggct gcgacgatct
    11281 ccggatcggc tccctcgtcg gcaacgacga gaatttcact tggaccggca atggagtcga
    11341 tgtcgacgac gccaaacacc aagcgctttg cgcacgcgac gtagatgttg ccggggccca
    11401 cgaccttgtc gacgcgcgca accgactctg tgccgtacgc gacggcggcg atagcctgcg
    11461 cgccacctgc atcgacgacc tcggtgacgc cggcgacctc ggctgcagcg aggatctcgg
    11521 gcgtgggatt gggcgtgacg agaacgatcc gctcgactcc cgcgacggtc gccgggatcg
    11581 ccgtcatcag cacggtcgac ggatagcgcg ccttgccacc cggcgcgtac accgctgcgg
    11641 cggcgaccgg ccgaacgcgc tgaccgagct cgatgccgtc ttcttcgtaa cgaaaacccg
    11701 ggtctcgttg atgctcgtga tagcgacgaa tcctttcgcc ggcgatcgta agcgcttcgc
    11761 gcaccgctgg atccacggtg cgggccgctt tcaaccaatc ttcgcgtttc agcaccagcg
    11821 aatccggacg ccgaccgtcg aagcgctcgg tgtactccag aacggctcga tcgccccgct
    11881 cgcggactgc atcgagaatc gcgcgcacgt cgggctctac gcgggcgaga tcttcgtcgc
    11941 cgcgacggct caaagacgct agcgtgtcgt cgtagccgga ttcgccccga gcgtagatgg
    12001 tgagcatttg ctgtttctag ccgccggaac aatggggtca accatgcaag cttgccctct
    12061 ggggcggaaa gtgctcagac tgcgccgacg atgaatgtcc gcttgtacaa caccctgacg
    12121 ggccaaaaag agcccttcaa acccgctgag gcgaaccacg ctcggatcta cgtctgcggg
    12181 ccgacggtct acgactacgc acacctcggc catgcccgct gctatgtcgt ctatgacgtc
    12241 ttggttcgcc acctgcgcgc cagcggaatg ggggtcacct acgtccgaaa cgtcaccgac
    12301 gtggatgaca agatcctgaa gcgagccgcg gagtcgggaa cagagcccac cgagctcgcg
    12361 acgcgctttg caaacgcata ctcggaagac atgcatcgac tcggaaacct ggatcccgac
    12421 gtcgaaccgc gtgtgagcac gcatctcgaa gacatctgca acctggtgca acgactggtc
    12481 gacggaggcc atgcctacct atcggacggc gatgtctatt tctcggtcga gtcgtttccc
    12541 gaatacggca agctgagcca ccgggacctg gggcaaatga agctcggcgc aagcgaccgg
    12601 ctcgacgaga gacaaaccgc gcgcaagcgc catccggccg acttcgccct gtggaagaag
    12661 agcgaaaaag atgcctgggg atgggatagt ccctggggcc gcggtcgtcc cgggtggcac
    12721 atcgaatgct cggcgatgtc gatgaaacat ctcggagaca ccttggactt gcacggcgga
    12781 ggcctggatc tcgtgttccc gcaccacgag aacgaaatcg cccaaagcga ggctgcaacg
    12841 gggaagcaat tcgcgcgatg ctggatgcac aacggcttcg tcgaggtcga caaagaaaag
    12901 atgagcaaga gcctcggcaa tttcttcacc gcgcgcgacc tgttcgatcg cgtcgagccc
    12961 gaggcgattc gttacttcat gatgacggtt cactaccgcg cccccctcaa tctcgattgg
    13021 acgctcgacg acgctggcaa cgtgaccggg tttccccagt tcgaagacgc tgagcgccgg
    13081 ctcgcgtacc tctacaagac caagcagcgg ctcgaaagcc ttccgcccaa tcgggtggtc
    13141 gatcaggatg gggcgactgc gccggaaatc gcaggattca gcaaggccct gcgcgagtcc
    13201 ctggatgacg acctcaacat gcccatagcg ctcgccaagc tcgccgactt cctgaaggcg
    13261 gtcaacgagc tcggcgaccg ggcgatgaga aagaagggca aggcttcccg ggccgcggtg
    13321 gagcaggccg gggcggggtt ccgagcgctc gaggcggagc tcggcatcgg agcggagccg
    13381 gccgaccaaa ttctcacgag aattcgggac cggagggcga aggctcgggg actggacgct
    13441 tccgtcatcg aggggcgaat cgctgaccgc acggcagcgc gaaaatccaa ggacttcgga
    13501 gaagcggacc ggatccgcga cgagctggct gcccaaggcg tcgagttgct agacagcgca
    13561 gaagggaccg gttggacgat ctcggactga tggtatcatc aggtgggtgc gggtagccat
    13621 tccggaccga cccgtgtgca atccaatcga tcgggtgcat atctgtcact cgcagcttcg
    13681 ttatggcacc ctgatatttc cttggagcat cgagctttga cgtcctctgt cccaaaaccg
    13741 ctggcggaag gcacactgga ggccacgcca ttcggtcacg tgcttctgtc gatccagaag
    13801 aagggcctgt cggggaccct cgccgtctgg cccgaggagg agcgctcagg acaggaccgg
    13861 atcctatttc gctctggggt gcccgcggtg ggccggttcc tcgatccggc ggcggacctc
    13921 gaacgaggca tactcaacat cttccatcgg cagaacgcgc cgtatgcgtt ctacgaggcg
    13981 gatctcgtag gcgatagcgc gggcgccctg cgcggtcaga cagacatcct ggctttgatc
    14041 gcgacctcgc ttcgcgctga tgtcccgaag gacgcgatgg tgcgggtgat tggaaagctc
    14101 ggcctcgagc ggcaacgcgt caagacggga gcgccgttac cgaggctcgg actgcagtcg
    14161 aacgagatgg ccttcgtcga gctgatgcgt gctgagcccg ccacggcagc gattctcatc
    14221 gagcagttcg gtgacccaaa ggtcgctcag cgcatgctct acttgctcgc gatcacggaa
    14281 tgcctggagc catatcgacc ggtgtcgcag cagaagctca gggccgtctc gccgccgcct
    14341 caaggtgcgc agcgttcatc gacaccacct gctgcgcgaa tctccgtcac tccaccggcg
    14401 agctcaggga gcgcatttcc atcgaggggc cgctcttcga gcccacccgg gttgctagat
    14461 tcgatgttgc ccggaggcga ttcggttctc ccgaaatcgc taagcaacct gcctccggca
    14521 aagacgctat ccggtgagat cgtcgcgcct cgatccctgc cacccgagcc tccggtcgat
    14581 gtcgaccccg agttgaagtc gcgctgggaa gaagtcgctc gcgtcatcag catgatggac
    14641 cgtcagaact acttccaaat gcttggcgca aaggaatccg aagacagcag cgggatcaga
    14701 acgaagtaca tgacccttgc aaagaagtgg cacccggatc gattgcctcc ggaccttgcc
    14761 gcgctgcgcc cctgggtgga ggagatcttt cacctcctga ccgtcgcgaa cgatacactc
    14821 agcgacacga agaagcgcgc ggagtatcaa cgggccgtaa tgcagggcgg cggcaccccc
    14881 gaatccgaac gaaagctcaa cgtcgtggtg gaagcggcca tcaacttcca aaaggtggat
    14941 atcctggtca agcggaagcg ctacgacgac gccctgaaga tctgcgacgc cgcgatggcc
    15001 gtcgttcgaa aagaagccga ctaccccgcc atgaaggctt ggatcctcat gctgagagac
    15061 ggtgtcgacg acgacgaagt cgtcgacgag atccgcggcc ttctcaggac gacctttgcg
    15121 atcaatcccg accacgtaca cggtcactac gtgcgcgcac atttcctcaa gaggcggggc
    15181 caacacgaca aggcgctcaa gcacttcaag aaggtggcga agctcgaccc caagaacctc
    15241 gaagcagccc gcgaggttcg ggtcggcacg atgcgtcagt ccagaggacg aagcagcgcc
    15301 ccaccccctc gcggcggtgg caaacgaccg agtctcttcg gtaagttctt caagaagtag
    15361 acctctgacg agcgtgcgtg agagctcggc ccggtgttgg gccgaaccag gacttcaggc
    15421 ttcccgaatc agaagctctc gagagtttcg tggactcgcg cgtcgtgcgc gggaactatg
    15481 atgagctctg ggttcgcgcg ctgcagttga tgcaggagca cgagttgctc gcgcactccc
    15541 gccgggtcgt ggtcgaccat gcgacgagcg agccaaggct tttcggcggg ccagtcgacg
    15601 ccttctttgg tccaagaggt gtccccgacg atgagaaatc gctgtccgtt tggtaggctc
    15661 acgaagaccc cgatcgaacc cggcgtatgc ccgggcatgg ggaccaatac gacgcttcca
    15721 tcctgaaaga aatcgtggct ggccgaaaaa ggaccgtagg caccgctagg gtaagtcagc
    15781 acctcgagtc ggaaagggcc ctctgcctcg agctgcctgt agagcttgcc gccttcgtct
    15841 tccgaacggg catgcgcgag ctcttcggga gcgatccaaa ccggaacctc gcgaagatct
    15901 gcgagcccgc tgacgtgatc ccagtgcgag tgcgtgagca cgattccccg aaggtcgctc
    15961 ggcgcaagat cgcgcgccgc aagcgcgtcg atcgtcggcc gctggaccgt caagctcgcg
    16021 agcgttcgca gcagcaaggg tgtggttttc aaatgctcgg aaacgtcgcg accgacgccg
    16081 gcgtcgatga gcaagcgtcc acggggatgc tcgaccacga aggcgaccag accactggtg
    16141 aacggcgtcg tcaatgaacc gccgcggaca ctgaatcctt cttggctctt catctccgcg
    16201 ccgcgaatga cgccaaaggt gacggagtcg acctccacgc ttggcggaag ccggggaccc
    16261 atggtcttgg gaacgggcaa gggtcgcttg acgaaggtcc aggcgagcga agtgacgagc
    16321 cctgcgatga tcaatgcggc gatcctggtc ttcatgcagc gacgaccata gccctgcgta
    16381 cccccgacgt cgcccctgga ccgggaatcg gtgggcgccg gcgggccgag gcgcgcgcca
    16441 ggtggggctc ggcgaagccc cgggaatctg ggtctcttgg actggcacgg gtgctgcact
    16501 cgactgcggc atgaacggcc cgcgggaacg actggctcac gacggcgtga agtccctttc
    16561 ggacgcggac ttgctcgcag ttctgctggg caccggcccc gccggcgagg atgtgatcac
    16621 cctggccagc agggttctga aagaagcggg cggactctgg gcgctggcgc ggatgggcgc
    16681 gaccgagctc gctcgtattc gcggcatcgg acccgcaaaa gcggcgcgct tggccgccgc
    16741 attcgaggcg gggacgcgcg gcttggtttc acctgacacg agtacccaac ctctttcgaa
    16801 cagcgagctc gtgttcctca gatatggtcg tccgctcacg acaagcgcca ttgaacgctt
    16861 cgtggtgatc tccgtcgatg cgaagaatcg agctcgcgcc gcacgcgagg tcgcccgagg
    16921 gggtcgagtc tcttgccagg tggatccggc ggaggtcttc aggctcttgg tctccgaatc
    16981 ggcctcgggc gcgatcttcc tgcacaacca tccaagcggc gacccggagc ccagccctca
    17041 ggacctcgag ctcactgagc ggctcgtcgc aggaggttcc ctgctcggaa tccgcatcct
    17101 cgatcacctc atcattggcg gtgggaggta ccgcagcctc agagacctcg atctctggcc
    17161 aagtaacctt gcaaaatcaa gctcttccgc tcgtcgtcgg agctgatctg ggcgacttga
    17221 cactccgacg gcctcgtgct ctcttcccgc actcttattt ctcgggagca tggctgtgaa
    17281 gcggacctat caaccacatc gtaagcgccg tcttcggaca cacgggttcc gcgcgcgtat
    17341 gaagaccagg ggcgggcgga gcgtgatcaa cgctcgtcgt cgcaaggggc gaacgcgcct
    17401 caccgtgtcc tgctacaaga agtagccgga agcggtacaa aaatgcccca aacatcggaa
    17461 acgacggggg cttcgagtga cgaaccgcaa cggtttcgac gcggcgatcg gctcaagaag
    17521 cgttacgagt tcaggcaagc acaactccgc gggcgacgaa tccacacccc ccactttctc
    17581 atcgtcgtcc taccaaatgc actacaaaac acgcgacttg ggatcaccgt gacgaagaaa
    17641 gtgggcaccg cagtcgaacg aaaccgcatc aagcgggtgg tccgcgaggt gttccgtcga
    17701 aatcgcactc tgtttcccgc tggtcacgac ttggtcttca tcgcgaagcg gggttcgacc
    17761 gacatcgcat acgactcgct attgagcgaa gtggagcgcg ccgccaagaa gcttcagccg
    17821 gaggccgctc gatgatgcgg gccgcgctgc tttttctgat ccgcctctat caaatgacgc
    17881 tcgctcggct catcggcccg agttgccggt tcgagcccag ctgttcgcgc tataccgcca
    17941 cttgcatcga gcggttcggc gcactgcgcg ggtcgtggct tggtatcaag cgaatcggac
    18001 gctgtcaccc cttgaaccct ggcggcttcg atcccccacc gaacgtgaag cacacgcacg
    18061 atcatccccg cgaggcgcag catggatgat cgcaagcagc cgccaatcgc acttctaatg
    18121 ctgcctctgg tcgcgctcgg cgcgatgctg gcggttcaat atttcgcgcc acccaaggcg
    18181 gagaccgagg agcctcccac cgagctgacg gcgcccgtgc aagccgagca acctagcggc
    18241 gaaacggccc cagcgcagat cgcgcccagc gattctcgat cgagaaccga agcgcagacg
    18301 ctgtacacgc tagaaaacga tcgattcgct gcgacgttca ccaacctcaa caccgcaatg
    18361 gtgagcctgc gggtcaaggg ggagcgctac gtcgacaaga gcggcgaacc gttcgagctg
    18421 gtcaccaccg acaaggaggc gtacctgccg ctcgcgcctt cattcaccgg cgtaacgctt
    18481 ccccctgatg cgcgttggcg gatcgccgag tcatctggga gcaagctggt ttttcagtgg
    18541 gccggtgacg gcgtctccat cacacgcacc tgggagctcg ccgagccgcc gtaccagctt
    18601 cactcctcga tcgaagttcg gaacgacgac cccaacacca gatcgattgg actggggatc
    18661 acctcggccc actacgttcg gcgtgaggac gaaaagggcg gcttcatggc gcgaccctcc
    18721 cccgcactga gtcacgggat ctgccagcag ggggacgaaa cgattcgtga agaccgggac
    18781 ggccttctga gcgaggatga gacccgaccg cttcagcccc acagctatgg cccgggcgtc
    18841 gagtacgtcg cgctgaccag cgtctacttc gcgatcgtcg ctgcagccga aggcgagccc
    18901 gcggagcgct gcgacatgcg caccaccgaa cgtggtggaa ctgtggatga cccggacggc
    18961 agtctcttcg aagcacggct agcctatgca ccacgggaga tcggcccggg cgagacggcc
    19021 gcatttcgca cggccgtgta tgcagggccg aaggactacg acgcgctcga agggtttggg
    19081 cacggcgcct ccaacatcat cgatctcggc tggttcagct tcattgcgca cggaatgagc
    19141 tggctgctcc gcaccatcta cggcttcatc gggaattggg gagtggcgat catcctgctc
    19201 actctcctgg ttcgtgtcct gctcctaccc ttcgtgatcc cgcagtttcg caacatggcg
    19261 aagcagcgcg cgctcaaacc agagatcgac aagatcaacg agctctacaa ggatgatcgc
    19321 gagaagaagg gcgccgcgat gatggagcta tggcgcaaac acaaggtgaa cccgcttggt
    19381 ggttgtttgc ccgtgctgct ccagatgccg atctttttcg ccctctacca aacgctctcg
    19441 acgagcatcg agctctatca cgcgcccttc gtgctgtggt ggaccgacct ttcttctccc
    19501 gacccgttct acgtactgcc ggtcgttctc ggcgctctga tgttcattca gcagaagctc
    19561 actccggtgc agatggacgc gacccaggcg aaggtcatgc tttacgccat gcctttgatg
    19621 atgactttct tcatgctgct tctgcctacc ggcctctgcc tgtacatggt gacgagctcc
    19681 gcggtcggca tcacgcagca gcgctacatg tactggaaga tggaccaaga ggcgtcggcc
    19741 ttgagcgcca gctcggacga ccctgaggaa gacgataatc aagcggcgga gggttccaaa
    19801 gccgccgcgc ccaagcaggc caagccccgc agaaagaaga ggactcgtgg tggacgagct
    19861 taaaggtaga ccccgtgacg gagcgctgat tctcgatcag ccgacgcagg aggacccctt
    19921 gcagtatgca ggggacggca aggccgaaga ggcggctgaa atcctcgagg agctgatgaa
    19981 tctcatggac ctcgacgtac gcgtcgacat tcgctccgac ggcgagcgta tcgagctcga
    20041 cgtgacggga gaggattccg gacgggtgat cggcaagaag ggggccacgc tcgacgcact
    20101 gcagttcctc atcaacaaga tggtgaatcg ctttccggaa gggcgacgtc acgtcatagt
    20161 cgattcaggc gactaccggg aacgtcacga cgacggtcta gtatcgatgg ccaagcgcca
    20221 agcagaacgc gccgtcgctg aaggcaaggt ggttacgctt cagccaatga acgcgcgtga
    20281 ccgccgggtc atccacatgt cgctggccaa gttcgatggt gtgagcacga tgtcgcaagg
    20341 cgagggaact cggcggcgca ttcagattat acccgcacga cgtcggcgct aacaccagcc
    20401 gccggtatca atcgcggtgc gcgtaggcga gtatcgttct aggcgcactg ctcgacggaa
    20461 cttcgtagtc cagcctacgc gtcagccgaa gatcgccgat cggcgctggc tctgcgccgg
    20521 cggtcaagac ccagacttcg tccgcaagtc gcgctccgat cgtcaaccac tgctccggcg
    20581 aaaacgtggc gcgagagagg gcgacggtga acggcgtgcc ctcgacctta ggttccgtcg
    20641 gatcgacctt gatctcgagc gtcgtcacac gctctgccag gccaagcgtg cccaccgccg
    20701 ttcgcaaaaa cgcgacgcgg attcgcctcg gctccacgag cgttccgtgg agactcgcgt
    20761 ccgaaagcat gagcgggatc gagggtgcgc caaccccagc accgatgtcg acgatcgacg
    20821 ccgatgaagt gggccatgcc gcgcggatca actgcgcggc atcgagaaac agaatctccg
    20881 cgagctcgag gtcgctacgg gccgatgtaa gatcggttcg cgcgctccaa gccttcacca
    20941 aatcgaaata ggcgtcccaa ccgccgggct cggaaacgcc ggcagcgtcg cagacctttc
    21001 caatggcgcg agaaccgctc atggtcgccg ggcctcctgc caagcagcct tcacctcggc
    21061 cacgctcgca atgcgttcgt ctttgttgtg atgcatggtc cggccaacca cctctgcgat
    21121 ccgggcaggg acctcggcga ctgcgtcgag tggcaggcag tcgccggcgt cgatcttcat
    21181 cacgagtcga cccaggtgag aatctcgaaa cggtctctca gtgctgagcg cctcgtagag
    21241 aacgaccccg agtccgtaga cgtcgatccg ctgatcgacc gaggcctttc ccgtcaggac
    21301 ttccggagcc atgtaggctg gggttccgct caattcgccg gtcgcggtga ggcgatcgag
    21361 accaagtatc ttcgaggcgc cgaagtccac tagccgtggg tctccgtctt cgtgccaaat
    21421 gacgtttgcc ggcttcaaat cgccatggag gacgccatga tcgtgaacgg ccccgaggcc
    21481 tccgcaaaga gcgtcgacca atgcgtcggc cgcggtgatg gtgagggggc catgcaagag
    21541 acgtgtggcc agggtttccc cctcgacgta ctcggtgacc aagaagacct gtccgtcctc
    21601 gtcgacgccg agctcgagga gttgcacgat cgcaggatga tcgatcgctt tgagtatcgc
    21661 accttcacgg cgaaacctcc tgcgcatcga ctcgtctgct aggtactcgg ctcgaagcac
    21721 cttgaccgcg gctttggatc catcgagctg gtcggtggcc tcgaacacgt ttcccatcgc
    21781 tccgcgggcg atctccgcca ccaaccgata gcgacccgcg agtaccgttc caagtccgat
    21841 ggttttggta tccttcatgc ctcggagtgg aacatacctc tcttcgcaaa ctcgcccaac
    21901 tggcgtaggc tccgctcgtc catgtcgctg cccaatctcc atcgccccct cgcgaagctg
    21961 tacggggagc cacttccatc gtttcggatc gtaaagctgc gaggcgacgc ttcgacgcgg
    22021 agttactatc gagtgtatgc ggaaggttcg gccggcggat ttcccgaaag cgtgatcgtc
    22081 atgcgacttc cggacgacgc tttgggtagc gacgaaggag gcccgcagca cagcacccat
    22141 cggttgcctt tcgtcgaagt tgctgagctg ctccgttcga aggggctgcc ggttccgggg
    22201 atcttcgtcg aggacctcga aaacggaatt cttctgctgg aggacctggg ggacacgact
    22261 ttcgagcaag ggctccgcgg agtcccgagc tcggattggg aagggctcta tgcgcgagcg
    22321 atcgatctac tcgccgacct gcatgagcga tgcgctgacc tgccgaagga ctcgatcgtc
    22381 tccaggcgcc gctttgaccg agggctactc gaatgggagc tcgagcactt ccgggagtgg
    22441 ggattggagg cgctctgggg cccgctcgac ggactgcaaa gccggacatt gagccgcgca
    22501 ttctcggaca tcgtgctcga gatcgaggcg atgccctacg gattcgtgca cagggactat
    22561 cagtcgaaga acctcatgct tgggcccggc ggcgttctca cgctgatcga tttccaagat
    22621 gcgcttctcg gtccccgcgc atacgacctc gtggccctgc tgtgcgattc atacgtgtcg
    22681 attggactcg agcttcagga atccatgatc gaacattatg ccgcactgcg tcaaatccag
    22741 gtggcgacac tgagaagaga gttctggctc gtcgctcttc accgcaagct gaaggacgca
    22801 ggacgcttca tcttcatcga ccgcgttcga aagaacccag acttccttca gtggtaccca
    22861 caaagcatgg agtatgtggg gcgcgccgcg gcccaagcac aaggctttga gccactcgcc
    22921 gccgttctgg ggctgaagat tcgagggttt cccgatgagg tgcagaaacc ctcttcctcg
    22981 gtggaataga tcagcgggag cctgcgttca ccccaggagc gacggggggt gaaaccggac
    23041 accaaaccgt actctctcct ctcccaccat ccgacatcct tcactcccgg tttcaccccc
    23101 ctgccgtccg aaaggccggt atttcttgca ggcacccatg agcggggtag actttcgttt
    23161 cgccttcatg agaaccctct ttcaggcact ggcccttgcc gcgatactct ttctcgacgc
    23221 ggcatctgca gacgcggccg attgcatttg cgatcagctg tcgacggagg gttgtacgca
    23281 gagcgcgcaa gtgcgtgacg agcccatcgc gccgccgctc tggtgcgagc gcaccgacga
    23341 cccacgctgt atgcctgcga gcacgcacgg cacctcgacg agcacactgg tgccagtggc
    23401 catgagctgg gctcaaccga ttcgatgggg cgcgccgcct cgcaccggca tccaaatggc
    23461 tgtccgggtc gaaggcaaag cgcgtaccga acactcacgt cgagtggagc gacccccacg
    23521 ctagcgccgg tcgccccagt cggggtggcc gtccaggtat ttgggttcgt tcattttgaa
    23581 cgttgagttt gtttcaagct cgccgtgcaa acgcggcggg aggataaaga tatgagtaaa
    23641 ggtgcagcaa ttttactggc catcgtgatt gccataggtg cattcctggt cggacgtctg
    23701 gtgagctcac caggcgatac ggataatgga accgcggaag ccggtggaga agaagcaaag
    23761 gccgcggacg tagccatcgc cgatgacgtc gaacgctaca aggtgccagt caccgacgcg
    23821 caaccgtcca agggccccaa ggatgcgccg gtcacgatcg tcgagttcag cgagttccag
    23881 tgcccattct gcgcgcgcgt cttgccgacc atgaaagagg tcgtcgacac gtacggtgac
    23941 aaggttcgcg tggtttggcg caacaatccc cttgcattcc acaacaacgc cggcccagca
    24001 gccgccgtcg caatggaagc gtatgcacaa ggcggtagcg aaaaattctg ggaggttcac
    24061 gacgttctct tcgccaacca aaaagcgctc gatcgcgcgc aactcgagga gtacgcgcag
    24121 aaggccggcc tcgacatgga caaagtgaaa aaggccatcg atgagggcac tcactcgaag
    24181 gcgatcggcg atgatcagac cttggcgagc cagctcgggg ccaacggcac gccctacttc
    24241 ttcatcaacg ggcgtcagct ccgtggggcg atgcctttca cggcattcaa gaaggtcatc
    24301 gacgaggagc tcgccacggc ggaggcgctg acgaagcaag gcgtcaagcc cgagcagatg
    24361 tacgcgacac tcaccaaaga cggcctgacg aaggcggccg ccgcaaagcc tacgccgcag
    24421 ggagcgcagc caggacaacc cgatccaagc gccgtctaca aggtcccact caagggcgac
    24481 gagccgcaga agggtccgga tgacgcgctc gtaaccatcg tcgagatcag cgatttcgag
    24541 tgcccgttct gcggacgcgt ggagccgact ctcaaggagg tgctcgacaa gtacggcgac
    24601 gacgttcgag tcgtgtggct caacaatccg cttcccttcc acaggaacgc caagggcgca
    24661 gcgaacgctg ccctcgaagc gcatgcgcag aagggcaacg ccggcttctg ggccatgcac
    24721 gagaaaatgt tcgaaaatca gaaggctctg acgaccgaca acctcgagaa atgggccaag
    24781 gaactgggcc tgaacatggc tcaattcaag aaggcactcg cagacgacaa gtacggcaag
    24841 aagatcgagg agcagcaagc cctggcgagc agcctaggcg cacgtggcac cccggcgttc
    24901 ttcatcaacg gccgaaacct ccgaggtgct cagcccctta cagcgttcac cgcgctggtc
    24961 gatgaggagc tggtaaaggc caaggcgctc gtcgcaaagg gcacgcccaa ggcaaaggtc
    25021 tatgcgacca tcatcgccaa cggcgagacc ggtccgaaga ccgctcccgc ggcggctcag
    25081 cccgacgcga acaaggtcta cgacatcccg gttccgaaga aggcgccgac gaagggtgca
    25141 aagaacgcca aggtcatcat ccaggagttc agcgacttcc agtgcccctt ctgcggccgc
    25201 gtcaatccga cgatcgcgca gctgatgacg gagtacgggg acaaggtgaa gatcgtgtgg
    25261 cgggactacc cgctcccctt ccaccaggat gcccagccag ctgcgcaggc cgcccgtgag
    25321 gtgttcgcgc agaagggcga taaggcgttc tgggcgtatc acgatctcct ctttcagaac
    25381 caaaagactc tcacgcgaga aaacttggag aaattcgccg agcaggtcgg cggaatcgac
    25441 atgaaggcgt tcaaggcagc gctcgactcc ggcaagcaca aggccgcggt cgatgcggac
    25501 atagcggcgg tgagcaaggc cggcgcacgc atcggcacgc cgtcgttctt catcaatggc
    25561 aagttgctcc agggcgctca accctacccg gcgttcaagg ccgcggtcga cgaagccctc
    25621 aagaactagt cgcaccgaca cacttagaaa ggggcaggtt cgcaagtcgg gcctgcccct
    25681 tccttttgac acgaagaggt ggacacacta cattccgtct cgtccgaaaa accaaagtat
    25741 tttcaaggtg ttgacgcatg gtccgagcga aagctgccgc cgctaaacga gtctccaaga
    25801 agccgatcgt cgaagagctc tatttccgga gcgacagtgc ttatctctgc ctcctcttga
    25861 accgtcgaac gcggagtatt cgcgtgatcg actttcgggc gggcgcgctt cccgccaagc
    25921 gtctctacat ccaatcggtc gcgacccaag agaacgtcga gaaggtgatc acactcgtag
    25981 aaaaagacga ggtgtcttca tggactcggg ttgggttcgt tcgcgaaggc accattccgg
    26041 gtttctacaa gcgcagcgac ggtcacctct gcggatgcgt catcggtgac aagactgcat
    26101 cgatcgaagt gagcgatgca tccgccaagc tcaccgaacg gacgatcaac gctgccaaga
    26161 aagccgccag cgacatcccc gagaagatca agggcgcttc ggttcgaagc gcatccgaga
    26221 aagacgcgct cgccgcccgc gacgcggcat ggcggaagca gccggcattt ggctctttcg
    26281 acatgttcgg acgggacgcg cagcggatct atttcgacat gagtcttcgc cgcagtaaga
    26341 ccaactacct ctccgccgaa ttccaggact gctttggaca tgccctggtc gaggtcttgc
    26401 gtctaccgac caacgaaaac gagactttgg cggtgacggc cgcgctccga acacttggcg
    26461 acaaccttca ggagcgaggc atcgtatcga cgtttgcctt cgcacccaac gacaacgtgg
    26521 agatttccac cgctttcgtc gctgcgggct accgaaaaac cgggctcctc gctcagggaa
    26581 tcctggacgc cgatggcggt cggaaagacg caattctctg gaccctcaag ttcgcgggtg
    26641 cgctgggcga agagttcgag tgagccttga ccgccctgat tgggggccgc taggctcgtg
    26701 aacgagcaag gagacgcgca tgcgctcacg cgaggatatc gaatcgtact tggtccgcgc
    26761 cgacgtttcc tacgaagagg tcggtgacga cgggatgtgg ctggtgcatc acggcagcct
    26821 cagcgagaac atcgccatca tcgctgccgg tccattgcta ttgttccggg tgaaggtact
    26881 cgagctccgg gagatcgagg acaaggcatc actctatgaa cgacttctga gcctgaacgc
    26941 ttcagacctg gtgcatggcg cctacgggat ctctgacgac tcggtggtac tgacttgcgc
    27001 gcttcagctc gagaacctcg actacaacga gctccaaggg gttctcgatg atttttcact
    27061 cgccctagca aaccactacg agcaactgac caagttccgg atcgccacgt aaaggacacg
    27121 tgcaatgggg ttcctcagcc gtttggcaca actcatcaag agcaacatca acgacctcat
    27181 caacaagtcg gaggatccgg aaaagatgct gaatcaggtc atcgtggaca tgaatcagca
    27241 actggtggaa gcgaagaaac aagtcgcgat cgccatcgcc gacgaaaagc ggctctacaa
    27301 gcagctcggg aacgaagcga agaagtccga agactgggag aagaaggcca tgctcgcggt
    27361 tcgctcggga gacgacgagc tcgcgaaaga ggcactgctc cgccgcaaag aacacgacgc
    27421 aatcgcgcta cagtatcaag agcagtggga gcgtcagaag gcgttgaccg atcagttgaa
    27481 aaccgccctc aaggcgctca accacaagat cggtgaagcg cagcgcaaga agaacgtcct
    27541 catcgcccgc aagcggcgcg ccgaggcgat gaagaacatt caggaaacga tgagcggcct
    27601 ctccgacgcg agcgcgttcg agaccttcga tcgcatggcg gagcacatcg agcagatgga
    27661 ggccgaagcc gaagcgggtg ccgaggtcgc cgaagagcac accggcgaca tcctcaagca
    27721 ccgcttttcc gagctcgaag ccacggcggg cgcagacctg gagctcgagg ccctgaagcg
    27781 aaagatgggt ctgcttccct ctgagcctcc ggtcgtgagc gcccgcatcg cgagccccgg
    27841 cgaggcgtac gaggaagagg agctcgccga gctcgaggag gcgctcgagg agctcaagcg
    27901 gcgagagcgc tgagcttgac agaaggcccc ggccgcttaa gttgcgggcc gaatactcgc
    27961 tatgcccacg tacgactacc attgcgacca ctgcggccac gagttcgagc gtgagcagag
    28021 gatcaccgag aaccccgtca agaaatgccc ctcgtgcggc aagctcaagg tgcgccggat
    28081 gatcggcggc ggaggcttca tcctcaaggg cgg
//
LOCUS       DASRZF010000357         5552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4062093, whole genome shotgun sequence.
ACCESSION   DASRZF010000357 DASRZF010000000
VERSION     DASRZF010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 5552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5552
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4062093"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="VFG22_16000"
     CDS             complement(<1..814)
                     /locus_tag="VFG22_16000"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET6417801.1"
                     /translation="MTIKTIVVGTDLGESGHRAMQLAVPTARATGATLWLVHASEVGL
                     DHAWHELPAGARSAGEALRARLKGRFENSLQELEKERKRCEALGLSCQTACEEGRPWE
                     TILRTAVSVSADLIAVGDPAGAIGLPERILGSTAERVVAQAPCSVLVSCGRLREDYRG
                     ARIAVGVDFSAHGIQAVRWARDVAEGIEGGVDLFHVVPHPIAEAIMPREWPSVLEGLA
                     SAARSRLEQIVASEGLDSATGIHVLDGPVGKMLCNATVEVDAGLLFVGCRGQG"
     gene            complement(884..1315)
                     /locus_tag="VFG22_16005"
     CDS             complement(884..1315)
                     /locus_tag="VFG22_16005"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET6417802.1"
                     /translation="MAQLLVRDLMTQPAITVAPTSPLRESIRLMESHNIRHVAVVEDS
                     GRLLGLISQRDVLRSQEGSLSGVLSSEQLHMNRWIEARWVMTKEVRTVHSETPALEAA
                     LILRSHRYGCVPVVDRGMLVGIVTDSDFLEYVIHMLSDPGM"
     gene            1497..2924
                     /locus_tag="VFG22_16010"
     CDS             1497..2924
                     /locus_tag="VFG22_16010"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HET6417803.1"
                     /translation="MADTFYERLSALDATFLDVESKAAPMHVGAALIFDAKPLMLEHG
                     GLDIERLTRYTEAAVDSIPRYRQRVDWVPGFGHPVWVDDDRFNMHFHLRHTRLPLPGD
                     ERSLKRLVGRLFSQHLDRSRPLWEFWVVEGVENDRFALIIKVHHCMVDGVAGVQLLEA
                     LLQISPDAVEREPQDWRPRPAPSRAQLLLNEANHRVEGLRKLSHYMPRVRENFKGLSS
                     VLRSGMKPAPKTAFTEHDISPYRRFDWTSFSLDEVKAIKGALGGTINDAVVAVTTGAV
                     RRFLLRRGENVDAMKGFRTVLPVNTRKAGGLSVGNHVAMLLADLPISEPDPLLRLKEV
                     IRVTTELKKESNQTGGAELIEDVADLTTKRIVSELYRAAMRLRTYNLIITNVPGPPFP
                     LYMLGAPMKAIYPMVPLMQNQNLGIALFSYSGALHWGFNADWESFPDVHEFVEDTEAS
                     FAEYKQLAAMRAPELSENATSPAGP"
     gene            2973..4400
                     /locus_tag="VFG22_16015"
     CDS             2973..4400
                     /locus_tag="VFG22_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6417804.1"
                     /translation="MTTVNTAPNMSEISNPANATGSKGRVLIVDDEANARTALAELLD
                     DAGYSISTAADGRTALLQIEQVDPDVVLTDLKMPGMDGLSLIEQGRPMSPHTTFIVMT
                     AFATIDTAVQAIKLGAESYLTKPLELDAVMAIVDRALERTRLSREAAQLRERVDERFQ
                     FGNILGEHPSMQRLMKNVAQVARSRATVLIHGETGTGKELIAAAIHQNSTRKDKPFVR
                     LNCASLSETLLESELFGHERGSFTGASGRREGRFKQADGGTLFLDEVSEIPASVQIKL
                     LRFLQEREFERVGGNETLKVDVRVVAATNRNLKQRVDDGRFREDLYYRLKVVQLDVPP
                     LRVRRSDIPLLAYAFLRKYAAENNRSVQGLSEEALQHLMIYPWPGNVRELENAIERAV
                     VMCNASQIERDDLPTSAHGDMQNGSVMALIPGITMAELERIAILRTLDAVDGSTARAA
                     EILGISQRKIQYRVKEWGYQSPILD"
     gene            complement(4397..>5552)
                     /locus_tag="VFG22_16020"
     CDS             complement(4397..>5552)
                     /locus_tag="VFG22_16020"
                     /inference="COORDINATES: protein motif:HMM:NF022995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protoglobin domain-containing protein"
                     /protein_id="HET6417805.1"
                     /translation="LKDYVGFDAESARRLRAMGPLMRASHHRIVDRFYEAILADPGAR
                     SVLKDQAQVVRIRGSLLHWLEGLFEGEYDEAYFEKRAKIGRVHVKVGLEQRYMLAAMN
                     VVRTGLHQALAEASGDSFGEFKDHCAIDQICDIELAIMLETYREDYVLKKTAEAESLA
                     VMGKLTAGLAHEIRNPLNAAKLQLDVLRRTAASVGDASTRRKIERRTNVVQDELRRLS
                     LLLDDFLNLAKARRLEPVECDAYALLEEVVELRRPEIESQGIEFIEDFAPASCALVAE
                     RDRLKQVVNNLITNAVEAVSDSSRPTIRITSRMLGEGRWEVAICDNGPGIPVDLAERA
                     FESFVTTKEAGTGLGLAIVKRIVDLHGGSATLTPLPEGGTRASFWVPAAP"
BASE COUNT         1155 a         1755 c         1606 g         1036 t
ORIGIN      
        1 gaccttggcc cctgcagccg acgaaaagaa ggcctgcatc gacctcgacg gtggcgttgc
       61 aaagcatctt gccaaccggc ccatcgagga catgaattcc agtcgccgag tctaatccct
      121 cgctcgccac gatctgctcg agtcgggagc gcgcggccga tgcaagcccc tcgagaacgc
      181 tcggccactc ccgcggcatg atcgcttcgg cgatcggatg aggcactacg tggaagagat
      241 cgaccccgcc ttcaatacct tcggcgacgt cgcgcgccca acgaacggcc tgaattccat
      301 gcgccgaaaa atcgacaccg accgcgatgc gcgcacctct gtagtcctca cgaaggcggc
      361 cacaggaaac cagcacggag caaggtgcct gcgcgacgac ccgctcagcg gtggagccga
      421 gtatacgctc gggaaggccg atcgctcccg cgggatctcc gacggcgatc aggtcggcgc
      481 tcaccgaaac cgccgttcgc aagatcgttt cccagggacg accctcttcg caggcagtct
      541 gacagctcag gccgagcgcc tcacagcgct tgcgctcttt ctcgagctct tgcaaggagt
      601 tctcgaagcg ccccttgagg cgtgctcgta gcgcctcgcc cgcagatctc gcaccggcgg
      661 ggagctcatg ccaagcatga tccaagccca cctcgctcgc atgcaccagc cagagggttg
      721 ccccggtcgc acgtgccgtc ggaacggcga gctgcatcgc gcggtgccca gactcaccga
      781 ggtcggttcc cacgacgatg gttttgatgg tcatatcgga atcagcggag caaacaccgt
      841 gccaggtccg gaacgacagc tccgcaactc cacgaccgcg ggctcacatc ccggggtcgg
      901 aaagcatgtg gatcacgtat tccaagaaat ccgagtcggt tacgatgccc acgagcatcc
      961 ctcgatcgac caccggcacg cagccgtatc gatgagatcg caggatgagg gcagcctcca
     1021 acgcaggagt ctccgaatga accgtgcgca cttctttcgt catcacccat cgggcttcga
     1081 tccaacgatt catgtgaagt tgttcgctcg agagcacacc cgagagactc ccttcttgac
     1141 tgcgcagcac atcacgctgc gaaatcaggc ccagcaggcg cccagaatcc tccaccaccg
     1201 cgacgtgacg tatgttgtgg ctctccatca gccgaatcga ttcgcggagc ggactcgtcg
     1261 gagcgaccgt gatcgccggc tgcgtcatca agtctcggac caaaagctgc gccatgaaca
     1321 acccctgttc gcatgtttta tgccagggct agccagttgg gtccgaaacg catattatgc
     1381 gcacattaaa attgtggccg caggatttgc gcgtggttat caggaatcca cgcataacca
     1441 tcggaatctc ggcaatgagg ttctcaggct tacattttgc tagtagtcat tccgccatgg
     1501 ccgacacttt ctatgagcgc ctgagcgcgc tggatgcgac gttcctcgac gtcgagagca
     1561 aggcagcccc catgcacgtg ggcgcagcgc tgatctttga tgcaaagccg ctgatgctcg
     1621 agcatggagg cctcgatatc gagcgtctga ctcgatacac cgaggctgca gtggattcga
     1681 ttccccgcta tcgccagcgg gtcgattggg tgcccgggtt cggccacccg gtctgggtgg
     1741 atgacgaccg tttcaacatg cacttccacc tccggcacac gaggcttccg cttcccggcg
     1801 acgagcgctc gctcaagcgc ctcgtcggca gactgttttc gcagcacctg gaccgcagca
     1861 gaccactctg ggagttctgg gtcgtcgaag gcgtcgagaa cgatcgattc gcgctgatca
     1921 tcaaggtgca ccactgcatg gtggatggtg tcgcaggcgt tcagctgctg gaagccttgc
     1981 tgcaaatcag ccccgacgcc gtcgagcgcg agccccagga ttggcgccca agaccggctc
     2041 cgagccgcgc gcagctactc ctgaacgaag cgaaccaccg ggtcgaaggg cttcggaagc
     2101 tcagtcatta catgccgcgc gtccgcgaga acttcaaggg tctatcgagc gtcctgcgtt
     2161 ccggaatgaa gccggctccg aagactgcat ttacggagca cgacatcagt ccatatcgtc
     2221 ggttcgactg gacttccttt tcactagatg aagtgaaggc gatcaagggc gccttgggcg
     2281 ggacgatcaa cgacgcggtc gtcgccgtca cgaccggtgc agttcgtcgc ttcctcctgc
     2341 gccgtggtga gaacgtcgat gcaatgaagg gattccgcac ggtgctgccc gtgaacacca
     2401 ggaaggccgg cgggctttcg gttgggaatc acgtagccat gctcctcgcg gaccttccca
     2461 tctcagaacc cgacccgctg cttcgcctca aagaggtcat ccgcgtaacg acggagttga
     2521 agaaagaatc gaaccaaacc ggcggggccg agctgatcga agacgtggca gacctcacga
     2581 cgaagaggat cgtgtcagag ctgtatcgcg cggcgatgcg actgcgcacg tacaacttga
     2641 tcatcaccaa cgtgcctggg ccaccgttcc ccctctacat gctcggtgcg ccgatgaagg
     2701 ctatctaccc gatggttccg ctcatgcaga accaaaatct cggtattgca ctcttcagtt
     2761 actccggcgc gctccattgg ggcttcaacg ccgattggga atcttttccc gacgtccacg
     2821 agttcgtcga ggatacggaa gcgtcgttcg cagagtacaa gcagctcgcc gcgatgcgcg
     2881 cgccggagct atccgaaaac gcaacatccc ctgcagggcc ttagaaacca agacgcctca
     2941 ttctggcagc tgacttgaac tagaagatga ccatgacaac tgtgaacacc gcaccgaaca
     3001 tgtccgagat ttcgaaccca gcgaatgcaa ccgggtccaa aggtcgcgtg ctcatcgtcg
     3061 acgacgaagc gaacgcccgc actgcgctcg cggagctctt ggatgatgct ggatattcga
     3121 taagcaccgc agccgacgga cggactgccc ttttgcagat tgagcaagtc gacccagacg
     3181 tcgtgctgac cgatctcaag atgcccggca tggacggatt gtcgctcatc gagcaggggc
     3241 gcccgatgtc gccccatacg acgttcatcg tgatgacggc gtttgccacc atcgacacgg
     3301 cggtccaagc gatcaagctg ggcgctgaga gctacctcac caaacccctc gagctcgacg
     3361 cagtgatggc gatcgtcgac cgagcgctcg agcggacccg actatcgcgc gaagccgcgc
     3421 agcttcgtga gcgcgtggac gagcgattcc agtttggcaa catcttgggc gagcacccgt
     3481 cgatgcagcg cctgatgaag aacgttgctc aagtcgctcg gagtcgcgca acggttctga
     3541 tccacggcga aaccggcacg ggcaaggagc tgatcgcggc tgccatccac cagaattcca
     3601 ccagaaaaga caagcccttc gtccgattga attgcgcgtc attgagcgaa accttgctcg
     3661 aatcggagtt gttcggacac gaacgcgggt cgtttaccgg agcgtcggga aggcgcgaag
     3721 gacgattcaa gcaggccgac ggaggcacct tgttcctcga cgaggtcagc gagatcccgg
     3781 cgagcgtcca gatcaagctt cttcgctttc tgcaggaacg cgagttcgag agggtcggcg
     3841 gcaacgaaac tctcaaagtt gatgttcggg tggttgctgc gacgaaccgc aacctcaagc
     3901 agcgcgtgga cgacgggcgc tttcgcgaag atctctacta caggctcaag gtcgtccagc
     3961 tagacgtccc cccactccgc gtacgccgca gcgacatacc cttgctggcc tatgcattct
     4021 tgcgaaagta cgctgcagaa aacaatcgat ccgtgcaggg gctctcggaa gaggcgcttc
     4081 aacacctgat gatctatccc tggcccggca acgttcgcga gctcgagaat gcgatcgagc
     4141 gcgccgtggt catgtgcaac gcaagccaga tcgagcgaga tgacctaccg acctcggcgc
     4201 acggtgatat gcagaacggc tctgtgatgg cgctcattcc gggcatcacg atggctgagc
     4261 tcgaaaggat cgcgattctc cgaaccctgg atgccgtgga tgggtccacc gcgcgagcgg
     4321 ccgagattct cgggatcagt cagagaaaga ttcagtaccg cgtcaaagaa tggggctacc
     4381 aatcgcccat tctcgactag ggcgccgcgg gcacccagaa tgaggcccgt gtaccgccct
     4441 cgggcagcgg ggtcagcgtc gcggaaccgc catgtaagtc gacgattcgc ttcactatcg
     4501 ccagaccgag cccggtgcca gcctccttcg tcgtgacgaa cgactcgaag gctcgctccg
     4561 ccaagtctac tggaattccc ggaccgttgt cgcaaatggc tacctcccat cttccttctc
     4621 cgagcattcg gctcgtgatg cgaatcgttg gccggctgga atcggagacc gcttccaccg
     4681 cattggtgat gaggttgttg accacctgtt tgaggcgatc tcgctccgcg acgagcgcgc
     4741 acgaagccgg tgcgaaatcc tcgatgaact cgatcccctg ggattcgatc tcgggacgcc
     4801 ggagctcgac gacctcttcg agcaacgcat acgcatcgca ctccacgggt tcgagtcgcc
     4861 ttgccttcgc caaattcagg aagtcgtcaa gcaataggga cagtctgcgc agctcatcct
     4921 gaaccacgtt agtgcgtctc tcgatcttgc ggcgagtcga cgcatctccg acgctggccg
     4981 cggtccgacg cagaacgtcc aactgaagct ttgccgcgtt caggggattg cgaatctcgt
     5041 gcgccaggcc ggcggtcagc ttacccatca ctgcgagtga ttccgcctcg gcggtcttct
     5101 tgagaacata gtcttcgcga tacgtctcga gcatgatcgc gagctcgata tcgcaaatct
     5161 ggtcgatcgc gcagtggtcc ttgaactcac cgaagctgtc cccggacgct tctgccaagg
     5221 cttgatgcag cccagttcga accacgttca tcgcggcgag catgtaacgc tgttcgaggc
     5281 ccacctttac gtggacccgc ccgatcttcg ctcgcttttc gaaatacgcc tcatcgtact
     5341 cgccctcgaa cagcccttcg agccaatgca gaagagatcc tctgatgcgc acaacttgcg
     5401 cttggtcctt cagcaccgaa cgagctccgg gatcggcgag tatggcctcg tagaagcggt
     5461 ccacgatacg atgatgcgag gcacgcatga gagggcccat ggcccgaagc cgtcgggcgg
     5521 actcggcatc gaagcccaca tagtccttca gc
//
LOCUS       DASRZF010000358         3429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_963599, whole genome shotgun sequence.
ACCESSION   DASRZF010000358 DASRZF010000000
VERSION     DASRZF010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963599"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VFG22_16025"
     CDS             <1..236
                     /locus_tag="VFG22_16025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417806.1"
                     /translation="SSVGESRFFIIFGLLQAWSIAAGFVLGVGFTIGSAFDVIVLLAC
                     SVLALSTSRRVHVAGLAVVWIAYGASAVLGASV"
     gene            377..649
                     /locus_tag="VFG22_16030"
     CDS             377..649
                     /locus_tag="VFG22_16030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417807.1"
                     /translation="MTDARIPADRKMLNTLQALTGMRIGEACGRRWRDYDRATPGLGA
                     IHVWSQYDDRPLKTGRASHEKERFVPVHPVLAKALAEWRLGGFAEV"
     gene            698..1135
                     /locus_tag="VFG22_16035"
     CDS             698..1135
                     /locus_tag="VFG22_16035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417808.1"
                     /translation="MVARTQNKAGKDHRTDAEVLGVYVLGRLTHGLRRWFISTCRNAS
                     ARTEVVELMTHNAKGDVIDAYTSWEWSTLCGELSKLEVDLNPAKLIVLPVAKSETPEI
                     TAVSHQVFASDFATRDQSKMISDAYRWRRWESNSRGYTRFRRF"
     gene            complement(1553..2473)
                     /locus_tag="VFG22_16040"
     CDS             complement(1553..2473)
                     /locus_tag="VFG22_16040"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HET6417809.1"
                     /translation="MKRTSKRPKLTNADRAFWVALSRLWPDWQDALILVKPETVIRWH
                     RKGFKLYWTWKSRHRGGRPRIDAEIRMLVRRMARENPTWGAPRIHGELLMLGFNVSEA
                     TVSRYMPHRRKPPSQTWRSFLRNHAKDLVSVDFFVVPTATFRILYVFLVLEHDRRRIV
                     HFNVTEGPSARWTGQQLINALPYDSAPKYLIRDRDTIYGAAFVRRVRAMGIEQVLTAP
                     RSPWQNAYCERAIGTLCRECLDHNIVLGEQHLRRILREYLEYYHSSRTHLALDKDAPE
                     SRERESTDEGKVIALPMVGGLHHRYTRVAA"
     gene            complement(2828..>3429)
                     /locus_tag="VFG22_16045"
     CDS             complement(2828..>3429)
                     /locus_tag="VFG22_16045"
                     /inference="COORDINATES: protein motif:HMM:NF023649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HET6417810.1"
                     /translation="FSAFQRKEVYGTSGPRIRLRFFGGWDFSSKDTGRKDMAAVGYDR
                     GVPMGGDLTKAPTGKAPSFLIHAVKDPLDGNLDRVQVIKGWVDASGTSQERIYNVAWS
                     GERTLAANGDLGPVGNTVDVDKGIYTNDIGASQLVASWEDPDFDPEARAFYYVRVLQI
                     PTPRSSLYDTLALREDASKSGQPAFIQERAYSSPIWYTP"
BASE COUNT          653 a         1009 c         1117 g          650 t
ORIGIN      
        1 actcgagtgt cggtgagagc cgctttttca tcatattcgg tctgctgcag gcctggtcga
       61 tcgccgcggg ctttgttctg ggcgtcggat tcacgatagg cagcgctttc gatgtgattg
      121 ttcttctggc ttgttcggtg cttgcattgt cgacgagccg gcgtgttcac gtggcagggc
      181 ttgctgttgt gtggatcgcc tacggagcaa gcgcggtact cggcgccagc gtctagcgaa
      241 agcccacgtt acgtcgggtc aggtattgct tctcggcgtg cctgttgctc gagcgacgcc
      301 agatcgattg gaccggtccc cgaaaaagcg agggaaggga ggcatcccgt accggcgaca
      361 cgaagccatc gcattgatga ccgatgcgcg gatccctgcg gaccgaaaga tgctgaacac
      421 actccaggcg ctcacgggca tgcgcattgg cgaagcgtgc ggccgtcgct ggcgcgacta
      481 cgaccgcgcg actccggggc tcggggccat ccacgtgtgg agccagtacg acgatcggcc
      541 gctcaagacg ggccgggcct cgcacgagaa ggagcgcttc gtgcccgtgc acccggtgct
      601 cgccaaggcc ctggccgagt ggcgcctagg cggctttgcg gaggtctagg gcaggcctcc
      661 gagcggagac gacttcatcg tcccggaccc cgcaacgatg gttgcgagaa cgcagaacaa
      721 agcaggcaag gatcaccgta cggacgccga agtgctcggg gtctacgtcc tgggtaggct
      781 tacccacggc ttacgtcgct ggttcatctc gacgtgccgc aatgcgtccg cgcgcacgga
      841 ggtggtcgag ctgatgacgc acaacgcgaa gggcgacgtg atcgatgcct acacgtcgtg
      901 ggagtggtcg acgctatgcg gcgagctcag caagctggag gtcgatctca acccagccaa
      961 gctgatcgtt ttgccggtgg caaaaagtga aacacctgaa ataaccgcgg tttctcatca
     1021 ggtttttgcc agtgattttg ccacccgaga tcaaagcaaa atgatctcgg atgcttatcg
     1081 gtggaggcgg tgggaatcga acagccgggg atacacccgg ttccgacgat tctagaacgg
     1141 tttcgcggac aggtatcgat gagaacacgt ccaaagaaca agaacaaaac gcgttaccgg
     1201 acattcttgt gacaatgtca caaaccccct tgcccgatgt gaaccgggag ctcgcggagg
     1261 ccggcgcgat gttctcggtg ttcgatgcgc tgcgtggaaa gcgttagcgc ccgacatcgc
     1321 agccttgccc cgcggaagcg ccgcgcctgg gtgtcgttcc tcaaaacccc gtctgttgcg
     1381 agatgtgagc cgcgatcatt gcggaggcga ccaagcggac ggaggtcggg tcgtcaggtg
     1441 cgtcggtgac ggctcgaaac gcacgagcca agcgcgaaat ccgcctgcgc cccgcgcagc
     1501 acgcacagct caagctcatg tccgacgttc gttgtgggac ggagactctc gctcaggctg
     1561 cgactcgggt gtagcgatga tgcagcccgc cgaccatcgg cagcgcgatg accttccctt
     1621 cgtcggtgga ctcgcgctcg cgggattcgg gggcgtcctt atcgagcgcg aggtgggtcc
     1681 gggagctgtg atagtactcg aggtactcgc gcaggatgcg ccgcagatgc tgctctccga
     1741 ggacgatgtt gtggtcgagg cattcccggc aaagggtccc gattgcgcgc tcgcagtagg
     1801 cattctgcca cggagaccga ggcgcggtca ggacctgctc gattcccatg gcacggacgc
     1861 gacgaacgaa ggccgcgccg tagatcgtgt ctcggtcccg gatgaggtat ttcggggctg
     1921 aatcataggg caaggcgttg atcaattgct gacctgtcca ccgggcggac ggcccctcag
     1981 tgacgttgaa gtgtacgatg cgtcggcgat cgtgctcgag cacgaggaag acgtacagga
     2041 tgcgaaatgt ggccgtaggc acgacaaaga agtcgacgga aacgaggtct ttggcgtggt
     2101 ttcgcaggaa cgaacgccag gtctgcgatg gcggctttcg ccgatgcggc atgtaccgcg
     2161 atacggtggc ttctgagacg ttgaagccaa gcatgagcaa ctcgccgtgg atgcgaggcg
     2221 ctccccaagt cggattctcg cgggccattc gacggacgag cattcgaatc tccgcatcaa
     2281 tccggggacg acccccccgg tgtcggctct tccaggtcca atagagcttg aagcccttgc
     2341 ggtgccagcg gatcacggtc tcgggcttca cgaggattag tgcgtcttgc cagtccggcc
     2401 agagacgcga aagcgcgacc cagaatgcgc ggtccgcgtt ggtcagcttg gggcgcttgc
     2461 tcgtgcgttt cacgacggcc agctgttgtc gcaaagcgag gttctcgagc gcgagctcgc
     2521 gctgcgacct caggctcgat cgggcggtcg cgagtagggc ggacagcaaa tgaagcgtcg
     2581 gcgtcggcta gcttgatcct gagcgcggaa acgtcaatga tcgcgcggtc gacgaggttt
     2641 tgaggaggca caggcgacaa atgcccacag ggccaggtag tcatgtcgct ccatccgtct
     2701 cggcttgcta ccacggtcgc gtctgggaca ttgtctacaa ggcaacaatc acatgccaac
     2761 ccggacgctc gaccacggaa tccctggaat tctccaagtg acgcatgatc cttgtcttcc
     2821 tcgagaacta cggcgtgtac cagattggtg agctgtatgc gcgctcctga atgaacgccg
     2881 gctgaccgct cttcgaagcg tcctcccgca aggcgagcgt gtcgtagaga gaggatcgag
     2941 gagtcggaat ctgaagaacg cgcacgtagt agaacgctcg cgcctcggga tcgaagtccg
     3001 ggtcttccca ggaggccacc agctgggatg caccgatgtc gttggtgtag atccccttgt
     3061 cgacgtcgac cgtgttgccg accgggccga gatcgccgtt ggcagcgagg gtccgctcgc
     3121 ccgaccaggc cacgttgtag atcctctctt gcgacgttcc gctcgcgtcg acccagcctt
     3181 tgatcacctg aacacggtcg aggttgccgt cgagtgggtc tttgaccgcg tgaatcagga
     3241 acgagggcgc cttgcccgtc ggagccttcg tcaggtcacc acccatcgga acgcctcggt
     3301 cgtatcccac cgcggccatg tccttcctgc cggtgtcctt gctcgagaaa tcccagccac
     3361 cgaagaaccg aagccgaata cgcgggccgc tggtgccgta gacctctttt cgctgaaacg
     3421 ccgagaaca
//
LOCUS       DASRZF010000359         4100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_965600, whole genome shotgun sequence.
ACCESSION   DASRZF010000359 DASRZF010000000
VERSION     DASRZF010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965600"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VFG22_16050"
     CDS             <1..285
                     /locus_tag="VFG22_16050"
                     /inference="COORDINATES: protein motif:HMM:NF023044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspJ"
                     /protein_id="HET6417811.1"
                     /translation="EQNRIDEDPRRGGKSQILVENVEEFNLDYFDPLLSDWVQTWDTE
                     DILAQPNRLPSQVRIRLAIKDPRHRGKTQTFGTRVTIPLTYALNHANYNP"
     gene            282..1634
                     /locus_tag="VFG22_16055"
     CDS             282..1634
                     /locus_tag="VFG22_16055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417812.1"
                     /translation="MNDTSRKRRSKRSEEGFALIIAIATVAILSVMLTDMHETTGTAF
                     AVSTSQRDALQAEYMAKSAINLTRLLIAKEPEVRSFVNPLYKAATGRPAPQLPVWNFI
                     DELLSPFCTPEDQRSTLVQLGIDFGNTVGFDDLPGECHVVAVSENGKINVNDPLFLDG
                     ELARNSVANQLFSLTGGHLPESPYDALFNKQDENGTLTTRIDLITAVIDWWDQDIQRT
                     DFDPGAGITRTGGTGAEDDSFYQMRDDSYRNKNAAFDSLQELRLVRGFSDDFWATFVE
                     PVPNDPKSRIMTIYASGLVNINEANPQVLLGRICSYAPEVSLCVDPLEGLKFTQILTT
                     IRQLIPIPLFSRPTDLIDFAEGKGSDKDLYGMLTGFLGEDSELIFTPIEIPAEQRTAL
                     ARSLATSAQIFTIVATGLVGRSQVRIESVVNFHTRWVPPPPNTGRMPGLGVFHYYRVN
                     "
     gene            1702..3105
                     /gene="pilM"
                     /locus_tag="VFG22_16060"
     CDS             1702..3105
                     /gene="pilM"
                     /locus_tag="VFG22_16060"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HET6417813.1"
                     /translation="MRGSEMEAYAEAALPFSAESESYADLLAAAVAQVLAVGSRPPDR
                     VIASLNGESASLRFLDLPRGVEKKVADVLPGELGALLPFDIDDAIIDYQVVGRDESTI
                     QLMAAAAPRTSIATRLRELSAAGIDPRELAVGAASFDGLGALLGEAIANTTVVVVDVG
                     PKNTDFAVLSNGSCTFARTLSGGMDLVESGRRAQLGGALQRTLSSYRAQRSEEPSRVL
                     LAGETAPMESARQWLSEQLGIECGVPPLPPAPGADEDTLPKFAKAAALAARAISRRKQ
                     LDLRRGEFASAAAAGQLRQHMRLLAVCLTAIVLSFAISLLARYRVASAEHEQLTASLA
                     EVSRNLLGEEARSALHARELLTAGPRIDDPLPRFDAYDVLEAISESIPTDIQHDTRRL
                     LIEIDDSGDEGRFEIQGTVGSIAERDRLVDQLQSHRCFADVEKGSISTAVEDRKDYKI
                     VVKVECADVDPDEGKDE"
     gene            3107..3676
                     /gene="gspM"
                     /locus_tag="VFG22_16065"
     CDS             3107..3676
                     /gene="gspM"
                     /locus_tag="VFG22_16065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HET6417814.1"
                     /translation="MAFGDALDNVRSWADALSDRERNLLSMMAAVFMAIVIVVPMYIG
                     ISSISDIETENEEITQVLKDIQRSEPRLQQQKADRRAVQQLYNRKAPSLGGYLEERAE
                     QYGVSGLGITDQPKLDIGAYSRRSVRVSLPSVELRPLIEMLADIENSSYPISIEQIQM
                     TGGRMRTGYTVKLAVSAYDREGPVVPAEE"
     gene            3680..>4100
                     /gene="gspN"
                     /locus_tag="VFG22_16070"
     CDS             3680..>4100
                     /gene="gspN"
                     /locus_tag="VFG22_16070"
                     /inference="COORDINATES: protein motif:HMM:TIGR04411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspN"
                     /protein_id="HET6417815.1"
                     /translation="MERIPIKRILKWAGYPLFFFACFLFFAYKTFPYDRLAERLVQEA
                     QMRGYEIEITDLTNSGLTGLSFENFRVIMPPEEDGAPPIDIVFEELTVGTSLFSMLSD
                     TKSYTFNAELGGGDVEGDIALGADTMDLDVQIDDINLE"
BASE COUNT          869 a         1245 c         1194 g          792 t
ORIGIN      
        1 gaacagaatc gaatcgacga agacccccgt cgaggtggca agtcacagat cctcgtcgag
       61 aacgtcgagg aattcaatct cgactacttc gacccacttc tcagtgactg ggttcaaact
      121 tgggacaccg aagacatcct cgcccagccg aatcgcctcc cctcacaggt cagaattcgt
      181 ctggctatca aggacccgcg gcaccgtgga aagacgcaga ccttcggcac acgagtgacc
      241 attccactca cttacgccct caaccacgcg aactacaatc catgaacgac acgagcagaa
      301 aacggcgcag caagcgctcc gaggaaggct tcgccctcat catcgcaatc gcgacggtgg
      361 cgattctatc ggtaatgctc accgacatgc acgaaacgac cggcacagcg ttcgccgttt
      421 cgacgtcaca aagagacgcg cttcaggccg aatatatggc caagagcgcc atcaatctca
      481 cgcggttgct gattgcgaag gaaccagagg ttcgaagttt cgtcaatccg ctctacaaag
      541 ccgcgaccgg tcgaccggct cctcaattgc ccgtgtggaa cttcatcgac gagctcttgt
      601 ccccgttctg cacgcctgaa gaccagcgca gtactctggt ccaactcggg atcgacttcg
      661 gaaacacggt cgggttcgac gaccttcccg gtgagtgcca cgtcgtggcg gtcagcgaaa
      721 atggcaagat caacgtcaac gacccgttgt tcctcgatgg tgagctcgcg cgaaacagcg
      781 tggccaacca gctcttttct ctcacgggag ggcaccttcc cgagagtccg tacgacgcac
      841 ttttcaacaa gcaggacgag aacggcacgc tcaccacccg catcgacctc atcaccgcgg
      901 tcatcgactg gtgggatcag gacatccagc gcaccgattt cgaccctggc gctggcataa
      961 cccgaaccgg gggcaccgga gcggaggacg actcgttcta ccaaatgcgc gacgactcgt
     1021 atcgaaacaa gaatgccgcc ttcgactcac ttcaggagtt gcgattggtg cgcggcttct
     1081 cggacgattt ctgggcgacc ttcgtcgaac cagtgccaaa cgaccccaag tcccgaatca
     1141 tgacgatcta cgcgtcgggg ctcgtcaaca tcaacgaagc caaccctcag gtccttctcg
     1201 ggcggatttg ttcctacgct cccgaagtca gcctatgtgt ggatccgctg gaaggtctga
     1261 agttcactca gattctcacc acgatccggc agctcatccc gatccccctc ttcagccgtc
     1321 cgaccgacct aatcgacttt gccgaaggca agggtagcga caaggacctg tacggaatgc
     1381 tcacagggtt cttgggtgaa gacagcgagt tgattttcac gccaatcgaa attccggccg
     1441 agcaacgaac cgcccttgcg cgctcgctgg cgacctcagc ccagatcttc accatcgtag
     1501 ccaccggact cgtggggcgt tctcaagtgc gaatcgaatc ggtcgtcaac ttccacacga
     1561 ggtgggtacc accaccgccc aacaccggaa gaatgcctgg tttgggtgtt tttcactact
     1621 atcgggtaaa ttgaaatgtc tttggttctg ggaatagagg ttactccccg cgcagttcgc
     1681 ggcgcctttt tgaggacgac gatgcgcggc tccgagatgg aagcatacgc cgaagcggcg
     1741 ctgcccttct cggcggagtc cgagtcgtac gcggacctgc tggcggcggc ggtggcccag
     1801 gtgctggcgg tagggtcgcg gccgcccgat cgcgtcatcg catctttgaa cggtgagtct
     1861 gcgtcccttc ggtttttgga cttgccgcgt ggagtcgaaa agaaggtggc cgacgtgctc
     1921 ccgggtgagc ttggtgccct tcttcccttc gacatcgacg acgcaatcat cgactaccaa
     1981 gtcgtcgggc gcgatgaatc caccattcaa ctgatggcgg ccgcagcgcc tcgcacgagt
     2041 attgcgactc ggcttcggga gctaagtgcg gctggcatcg acccgcgaga gctggccgtg
     2101 ggagccgcat ccttcgacgg tcttggggca cttctgggtg aggcaattgc gaataccacc
     2161 gtagtcgtgg tcgacgtcgg cccgaagaac acggacttcg ccgttctcag caacggtagc
     2221 tgcacgtttg cgagaacgct gtcgggcggc atggatctgg tggagtccgg gcgtcgtgct
     2281 cagctaggcg gagctctcca acgtacgctt tcgagctacc gcgcgcagcg ctccgaggag
     2341 ccctctcgtg tcttgctcgc cggggaaacc gcgccgatgg agagcgcgcg tcaatggctc
     2401 agcgagcaac tcggcatcga gtgcggcgtc ccgccactcc cccctgcacc aggagcggat
     2461 gaagacactt tgccgaagtt cgccaaggcc gctgccctcg ccgcgcgagc gatctcgcgc
     2521 agaaagcaac tcgatctcag gcgaggagag ttcgcatcag ctgcggctgc gggccaactt
     2581 cggcaacaca tgcggctgct cgccgtctgc ttgaccgcca tcgttctttc gttcgctatt
     2641 tcgttgctgg cacgctaccg ggtggcaagc gccgagcacg agcagttgac cgcgtcgctg
     2701 gcggaagtta gccggaatct tctcggcgaa gaagcgcgca gcgcactgca cgcgcgtgaa
     2761 ttgctgactg cgggaccgag gatcgacgac ccgttgccac gcttcgacgc ctacgacgtg
     2821 ctcgaggcga tctccgaaag cattcccacg gatatccagc acgatactcg gcggcttctg
     2881 atcgagatcg acgactccgg cgacgagggt cgcttcgaaa tccaagggac ggtcggctcc
     2941 atcgcagagc gggacaggct cgtcgaccaa ctgcaatcac atcgttgctt cgcggacgtg
     3001 gagaaaggct cgatctcgac ggccgtggag gaccggaaag actacaagat cgtggtcaaa
     3061 gtcgaatgtg cggacgtgga tccggacgag gggaaggacg agtaggatgg ccttcggaga
     3121 cgccctagac aacgtacgga gctgggccga cgcattgagc gatcgcgagc gtaacttgct
     3181 gagcatgatg gccgctgtgt tcatggccat cgtgatcgtg gtgccgatgt atatcggcat
     3241 ttcgagcatc tcggacatcg agacggagaa cgaggaaatc acccaggtgt tgaaggacat
     3301 ccagcgatcc gaaccccgcc tgcagcagca gaaggcagat cggcgtgcgg tgcagcagct
     3361 gtacaaccgc aaggccccgt ctcttggtgg ttacctcgag gaacgagcag agcaatacgg
     3421 tgtgagcggc ctcgggatca ccgatcaacc caagctcgac atcggcgcgt acagccgacg
     3481 ttcggtgcga gtttccctgc catccgtcga gttgcgacca ctcatcgaga tgctcgccga
     3541 catcgaaaac agctcctacc ccatctcgat cgagcaaatt caaatgacgg gcggccgtat
     3601 gcgaaccggc tacaccgtga agttggcggt cagtgcatac gatcgcgaag gccccgtcgt
     3661 accggctgag gagtaggcgt tggaacgaat cccaatcaaa cggatcttga aatgggcggg
     3721 atacccgctg ttcttcttcg cgtgtttcct attcttcgcc tacaagacgt ttccctacga
     3781 tcgattggcg gaacgtctcg ttcaagaagc gcagatgaga ggctacgaga tcgagatcac
     3841 cgatctcacg aactccggtt tgactggtct cagttttgag aattttcggg tgatcatgcc
     3901 gcctgaagag gacggcgccc ctcctatcga catcgtcttc gaggagctca cggtcggcac
     3961 gtctctcttc tccatgctct ccgatacgaa gtcgtacacg ttcaatgcag agctcggggg
     4021 aggagacgtc gaaggtgata tcgcgctcgg tgccgacacc atggatctcg atgtgcaaat
     4081 tgacgatatc aacctcgagt
//
LOCUS       DASRZF010000360        10760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3373115, whole genome shotgun sequence.
ACCESSION   DASRZF010000360 DASRZF010000000
VERSION     DASRZF010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 10760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10760
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3373115"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(564..644)
                     /locus_tag="VFG22_16075"
     tRNA            complement(564..644)
                     /locus_tag="VFG22_16075"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(609..611),aa:Leu,seq:taa)
     gene            complement(663..1070)
                     /gene="panD"
                     /locus_tag="VFG22_16080"
     CDS             complement(663..1070)
                     /gene="panD"
                     /locus_tag="VFG22_16080"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HET6417816.1"
                     /translation="MRRRMFLGKIHRATITHADLDYEGSVTIDADLLDAAGILEHEEV
                     QIWNVTQGTRLATYALEGPRGSGVICVNGAAAHHMSPGDLAIIATFGEMTDKEARAHK
                     PKVVLVDSKNRIVDTGPERPGPRMPRRVDELIH"
     gene            complement(1193..1531)
                     /locus_tag="VFG22_16085"
     CDS             complement(1193..1531)
                     /locus_tag="VFG22_16085"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6417817.1"
                     /translation="MIPVADRLSSILPKGGADASIHRGARRQCRRWALDAEIDLLTPA
                     RGSGLAINASVGGLRVAVDTGVPVDQICTLRVRTAPDHETIEHARVVWSKAQPDGYVL
                     GLEFIAAPIS"
     gene            complement(1636..2541)
                     /locus_tag="VFG22_16090"
     CDS             complement(1636..2541)
                     /locus_tag="VFG22_16090"
                     /inference="COORDINATES: protein motif:HMM:NF038402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HET6417818.1"
                     /translation="MIEVVDAIDRHVQLGRPATRVVSLVPSETVSVADLVGMDRLVGR
                     TDYCVEPLGEVERVPSVGGTKGFDVAAVKALRPDLVLANKEENSRPQVQALIAAGVPV
                     HVSFPCTLDESIAYLENLCSLLGLDPDAATQVLDCRTAARRVALEQLTPPLPVFVPIW
                     KDPWMTFDRGAYASDILAACGANNVFSERVRRYPLAADLGKVSPWSEERVEARDTRYP
                     RIRLEEVLERGARVVLLPDEPYAFGPDDMKELSALAGSHPLIVERVDGKDLFWYGTRA
                     ARAIDRLSVRIKSLHSTCFGVRANN"
     gene            2597..3106
                     /locus_tag="VFG22_16095"
     CDS             2597..3106
                     /locus_tag="VFG22_16095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417819.1"
                     /translation="MPEICATRVEGGTFVHPKPGLAASGLEETLSIQDIQRALAEAGV
                     EIYRSEARELQIAERVRLHIMDSGVRVVLNGNLLVQFTARTQRSDAPSAQSEELFRRV
                     REEIGAKAGNRGYVEASAEIVEVRDPVDDARVLDVWHEVTYRKPLEAIGDAVTEVRWA
                     LELEKYVHP"
     gene            complement(3110..3334)
                     /locus_tag="VFG22_16100"
     CDS             complement(3110..3334)
                     /locus_tag="VFG22_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013859272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HET6417820.1"
                     /translation="METGLVKWFNNAKGWGFIAGRDGSDIFVHYSHIAGDGFRTLREG
                     QQVRYDLRQGPRGQFAENVQVNEELARAAS"
     gene            complement(3432..4013)
                     /gene="pdxH"
                     /locus_tag="VFG22_16105"
     CDS             complement(3432..4013)
                     /gene="pdxH"
                     /locus_tag="VFG22_16105"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00558.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HET6417821.1"
                     /translation="MSHEDPILRFAREYERAGASEEFEVSRCALATADAGGRPAVRFV
                     LLKGFDDDGFVFFTNLESPKAKDLAANPHAEIAFHWHTTGIQVRVRGPVSRVSDEESE
                     AYFATRDRGSQLGAWASRQSQVLENRESLEAAVIDVAKRFRGVSVERPPFWGGYRIAP
                     IAIEFWGNRDDRLHDRWLYERDGDSWRITQRYP"
     gene            complement(4010..5008)
                     /locus_tag="VFG22_16110"
     CDS             complement(4010..5008)
                     /locus_tag="VFG22_16110"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6417822.1"
                     /translation="MSERGDRDNVIRVGFGEDGKLTRAVTPEPAPTQPAPPRSDPRQG
                     LYTVAEVARLFGYKPSRLRYWHRSGLIGPSARVAGRNYYRFQDLVEVRVAKTLLDQGV
                     SLQQVRVSVESLRRSFPETVRPLSELRVLGDGGNVVVQDEEGKYEPSTGQAVFDFRID
                     GLESDVIRLLHFDHDDRSRAYAHYLEGCRCDEDEETYEQAEQAYRKALSLDPTLSNAL
                     TNLGNLEYRRDQPVAAEQYYRRALASDPEQPEALYNLGFLHFERDEIEEAVRLFRSAL
                     DSDPSFADAHFNLAMALEEQGERTQARPHWQRYLELEPDGAWSDLALKHLQQGTAP"
     gene            5104..5775
                     /locus_tag="VFG22_16115"
     CDS             5104..5775
                     /locus_tag="VFG22_16115"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET6417823.1"
                     /translation="MAHPPVAAPIAFLSDVHGNLEALEAVLSELERMGVKRLFVAGDL
                     LLGGEQPLEVWQRLQALGATCTRGPSDLALGSVDPGSLVPIDNEQRRQARLFAETRAA
                     IGDLVAERLRRLPEQQRIPLVDGREVLMVHGSPADSSHEIGHDLSDDEILSLLDDDPA
                     DIIVCGSTHVPFQRTVEEYHIVNVGSVGAAPEGRVAHYTVLTPRMSSADILQDWVSYE
                     EPDAS"
     gene            complement(5772..7127)
                     /locus_tag="VFG22_16120"
     CDS             complement(5772..7127)
                     /locus_tag="VFG22_16120"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HET6417824.1"
                     /translation="MRALEVLGNLRAHRTLDVPSTQLAVAMLGGVGLGALLAAVGKGL
                     HSLGEAGLHALSEADDRSARVAMRVLRDLDSVHARIFLGRALSLALTASFVGYSLLRS
                     SGLGVALAGIVGISLAYVVAGTIAIAVIRQRSGRATLRVWQWLRPFDLLLTPLAAPLN
                     WIRDLIERFVPMVQLDDPHRVTELTVEHVIEQGEEIGTIAEDHAQMLRSVLEFKNTVA
                     REIMVPRTQVVAFDIKTGIDEVSVQINESGHSRYPVYRGQIDQVEGILYAKDLFQALR
                     DSSDAANILLADVLRKPVFFAAESQKIGVLLREMQRRRSHLAVVVDEFGGAAGIVTLE
                     DIVEEIVGEIQDEHDEEAPLVYERAPGHYFVDASISVYDLEEHLGESICEDKSEFDSL
                     GGMLVHLLGQVPAIGETLLAGPYRFVVLDADERRVRRVEMLRVKSEEPGDAQEPKVAA
                     V"
     gene            complement(7137..8351)
                     /locus_tag="VFG22_16125"
     CDS             complement(7137..8351)
                     /locus_tag="VFG22_16125"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF001770.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HET6417825.1"
                     /translation="MAASPKKIVLAYSGGLDTSVILTWLKETYDAEVIAFCADVGQGE
                     ELSGLDEKARATGASKCIIADLKEEFARDFCFPMLRANAVYEGDYLLGTSIARPVIAK
                     AMMEVARQEGADAISHGATGKGNDQVRFELTAMAIDPRIQVIAPWRTWTLKSRSDCIA
                     YCKDRKIPIEVTAEKPYSMDANLFHISYEGGILEDTWNEPLKDMFRWTTDPQDAPDSP
                     EYVVIGYEKGDPVSVNGERLSPAVLIERLNTLGSKHGVGRVDVVENRFVGMKSRGVYE
                     TPGGTILQRAHRAVESITCDREVLRIRDGLIPEYASLVYRGFWFAPEREMLQALIDEA
                     QKDVTGEARIKLYKGSASVVGRRSPNSLYDPEFATFEADEVYDQQDATGFIRLNALRL
                     RIRALRDQLRDA"
     gene            complement(8400..9002)
                     /locus_tag="VFG22_16130"
     CDS             complement(8400..9002)
                     /locus_tag="VFG22_16130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417826.1"
                     /translation="MKSARPRSSDSKFPSVLGSDGAIDLTGGLGDRGIEERPTSRPSA
                     DAWVIDRDRRSLIPSASEVEPISLRGSDESSSHEIHRDIADHFALGNYAAALNAAELQ
                     LGLDPEDESARRYAENSRARLETRYTTRIGSLDYVFNLGVPAAKVRWLGLDPQASFLL
                     SLVDGQTTVAEVLDLCKMGRLEALRVFTELLEAKAIIRVA"
     gene            complement(9006..>10760)
                     /gene="dnaK"
                     /locus_tag="VFG22_16135"
     CDS             complement(9006..>10760)
                     /gene="dnaK"
                     /locus_tag="VFG22_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HET6417827.1"
                     /translation="IANRGGYKVTPSMVAVTEGGKRLVGHIAKRQAVTNAENTVFACK
                     RLIGRKWDTPQVKNAIQTTPFHIVEGPHGDCRIKMRDKVYSIPEISAMVLQEMKMVAE
                     DYLGEEVNKVVVTVPAYFNDNQRQATKDAGAIAGLDVIRIINEPTSAALAYGFGKNID
                     RTVAVYDLGGGTFDISILEIASTGVFKVISTAGDTFLGGEDFDQRVIDWLVEGFQHEH
                     GIDLRQDRMALQRLKDAAEKAKCELSAVTETEVNLPFIISSARNEALHLQRLLTRATL
                     EELTADLVDRTVQICELTLGEAGLQKDEIEDIILVGGMTRMPKVQERVSKFFQRDPCK
                     GVHPDEVVGLGASIQGAALVDHSPELDMVLLDVTPHTLGIMVVGGYFEELIPQNTTVP
                     TSRSKPFTTVRDNQTAMKILVLQGESRRAEDNELLGEFILTGLRRAAAGQVEVEVTFE
                     INADGIVSVHAKDVETGKEQSITVTATSGLTEDEIDEMMVDASDFAVTRREDEASERI
                     RQEAETLIAEIERLFPEVESIVAGSDFGRDAIEKAKGVVDNARTLLERGDLAGLKDEL
                     ESLGRTQRMFKGVVGKSA"
BASE COUNT         2048 a         3421 c         3225 g         2066 t
ORIGIN      
        1 atccggctgc agttcaaacg cgccgacgtc gcacctctct cctccttcat cgcaccccac
       61 catcagggcg aggctcagcg cgagtgcaaa ccaaaacctg aatccgtgtc gcatggccca
      121 cctccggttt cgtgcgcctg ccaccgatca aagggcaagc atcagccgtg ccagcccgtt
      181 ttcggagcgg tttccgttac cctgacgcgc acggtttgcg tttgctggga gggcggcgaa
      241 gggagtgcgt ggctatggct gcacctcaaa cgcacccagg tcgcacagcc cggagttagg
      301 ctcagagctc ggctggaagg caatatggga atgtgcccgg ctcttcgtgg gacgaccgcc
      361 acctaaccat cctggaagcc gcgttggcac tccacgcaga acgccgcctc gggcttggca
      421 gcaaggcgca actccgactc gtcgagtctt tgaagccttc ggcgccacct cacgtcaacc
      481 tcaggtcaac ggggaccgac gatgaaaagc gctaacgctg tcaagctccc tcaatcacga
      541 acgaagtgcg gatgccgtga tagtgccggg ggcgggattc gaacccgcac gtgttgccac
      601 gggcgatttt aagtcgcctg cgtctgccat tccgccaccc cggcgacgcg gcgagcatcg
      661 cgttaatgga tgagctcgtc cactcgtcgg ggcattcgcg ggcccggacg ctctgggcca
      721 gtgtcgacga ttcgattctt gctgtcgacg agcacaactt tgggcttgtg cgccctcgcc
      781 tctttgtccg tcatttcgcc aaaggtcgcg atgatcgcca gatctccagg gctcatgtga
      841 tgcgctgccg cgccgttgac acagatcacg ccggagcccc gtgggccctc gagcgcgtag
      901 gtcgccaagc gcgtgccctg cgtcacgttc caaatctgaa cttcttcatg ctcgaggatg
      961 ccggcggcat cgagcaggtc agcatcgatc gtcacgctgc cttcgtagtc caagtccgcg
     1021 tgggtgatcg tagctcggtg aatttttccg aggaacattc gtcggcgcat gtttttgaac
     1081 ctccgtagcg tcccggattg ggtacgctca gataacgaga agttaacccc ggtcgagcgc
     1141 cgccgcaagc aatcccatca tccgcgcagg cgcccccaaa aaccgcacaa atttaggaaa
     1201 taggcgccgc aatgaactcg agcccgagca cgtagccatc gggctgcgcc ttcgaccaaa
     1261 cgacccttgc gtgctcgatg gtttcgtggt ccggggcggt gcgaactcgg agtgtgcaga
     1321 tctggtcgac cggcacgccc gtgtcgacgg caactcgaag cccgccaacg cttgcgttga
     1381 tggccaagcc cgagccgcga gcaggagtga ggaggtcgat ctcggcatcg agcgcccaac
     1441 gccggcattg gcggcgtgcg ccgcgatgaa tggaggcgtc agcgccgccc ttcgggagaa
     1501 tcgaagagag tctgtctgca acgggtatca aagcatccgg aatgtatcga agcgtccgat
     1561 ccttggctag gcttttctgc ttgcgagcgc catgaaaagc gatgcgagtc tacccttgag
     1621 gcggagacac ccgggttagt tgttcgctcg cacaccgaaa cacgtcgagt gcaggctctt
     1681 gatgcgaacg ctgagccggt cgatcgcccg cgcggcgcgc gtcccgtacc agaacaagtc
     1741 tttcccgtcg acacgctcga cgatgagcgg atgagacccc gccagcgcag agagctcttt
     1801 catgtcgtcc ggaccaaatg cgtagggctc atcggggagc agcaccacgc gcgctcctcg
     1861 ctcgagcacc tcttccagcc gaattctcgg gtaccgtgtg tcccgtgctt caacacgctc
     1921 ctcactccag gggctgactt tcccaagatc cgcggccaaa gggtagcgac gcacacgttc
     1981 cgaaaacaca ttgttcgcgc cgcatgctgc gaggatatcg cttgcgtacg cgccgcggtc
     2041 gaatgtcatc cagggatctt tccagatcgg aacaaacacc ggtaacggcg gggtcaactg
     2101 ttccagtgcc acgcgccgcg cggccgtgcg gcagtccagc acctgcgtgg cagcatcggg
     2161 atcgagcccc agcaaggagc agaggttttc caaatacgca atcgattcat cgagcgtgca
     2221 gggaaagctc acatgaaccg gaacgcccgc agcgatcaag gcctgaacct gcggccggct
     2281 gttttcttct ttgttcgcaa ggacgagatc cggcctcagc gctttcacgg ccgccacgtc
     2341 gaatcccttc gtaccaccca ccgacggaac tctctccacc tcaccgagag gttccacaca
     2401 gtagtcggtt cgccctacga gccgatccat accgacgaga tctgcaaccg acacagtctc
     2461 gcttggaacg agcgaaacga cccgggttgc gggacgtccg agctgcacgt gccgatcaat
     2521 tgcatctacg acttcgatca tgggaccctt caggcgtccg agtgagacac ccggtcaatt
     2581 actagcactc cctcaaatgc ctgagatttg cgccacccgt gtcgaaggtg gtacgtttgt
     2641 ccacccaaaa cctgggcttg ctgcgagtgg gttggaggaa acgctgtcga ttcaggacat
     2701 tcaacgagcg ctggccgaag ccggtgtcga gatttaccga agcgaggctc gggagctcca
     2761 aatcgccgaa cgcgtgcgac ttcacatcat ggattcgggc gtgcgagtcg tgctcaacgg
     2821 caacctgctc gtgcagttta ctgcgcgaac ccagcgctcg gacgcgccct cagctcagtc
     2881 ggaagagctg tttcggcgcg tgcgggaaga gatcggcgcg aaggcaggga accggggata
     2941 cgtcgaagcc tctgctgaaa tcgtagaggt gcgggacccc gtcgatgacg cgcgtgtgct
     3001 cgatgtttgg catgaagtga cctatcgcaa gccgctcgag gccatagggg atgcagtcac
     3061 ggaagtccgc tgggcgctcg agctcgagaa gtacgtacat ccctgagagt cagctcgcgg
     3121 ctcgggctag ctcttcgttg acttgtacat tttcggcgaa ctgcccgcgc gggccttggc
     3181 gaaggtcata gcggacctgt tgcccttctc ggagcgtccg aaacccgtca cctgcgatgt
     3241 gggaatagtg cacgaagata tcggagccat cacggccggc gatgaacccc caacccttcg
     3301 cgttgttgaa ccacttgacc aatcctgttt ccatgacccc cagaacctcg atgtgagacc
     3361 actgtgttcc cccggttaga tcaaagatct ccagtcgacc ggtcttcgtc aaggatcaat
     3421 ctgcggctcg gctaggggta gcgctgcgtg atgcgccaac tgtcgccatc tcgctcgtag
     3481 agccagcgat cgtgcagacg atcgtcgcgg ttcccccaaa actcgatcgc gattggagcg
     3541 atgcggtagc cgccccaaaa cggagggcgc tcgaccgaaa caccacggaa acgtttcgca
     3601 acatcgatga ccgcagcttc gagcgactcg cggttctcga gaacctgact ctggcgcgaa
     3661 gcccatgcgc cgagctggct accccgatca cgggttgcaa aatatgcttc ggattcttcg
     3721 tccgacacgc ggctcaccgg gccgcgcact cgcacctgaa tgcccgtcgt gtgccaatga
     3781 aaggcgatct cggcgtgcgg gtttgcagcc aggtccttcg ccttcgggct ctcgaggttc
     3841 gtgaaaaaaa cgaacccgtc gtcatcgaac ccctttagca agacgaagcg cactgctggc
     3901 cgccctccgg cgtctgccgt cgccagcgca cagcggctga cttcgaactc ctcgctggcg
     3961 cccgcacgct cgtactcgcg cgcgaaacgc aagatcgggt cttcatgact catggtgccg
     4021 tgccctgctg caagtgcttc aaggcgaggt cggaccaggc tccgtcgggc tcgagctcga
     4081 ggtaccgttg ccaatgcggc ctcgcctgcg ttcgctcacc ttgctcctcg agcgccatgg
     4141 cgagattgaa atgcgcatcg gcaaacgaag ggtcgctatc gagcgcgctt cgaaaaagac
     4201 gcaccgcttc ttcgatctcg tcacgctcga agtggaggaa ccccaggttg tagagagcct
     4261 ccggttgctc gggatcggag gcgagcgcac ggcgatagta ctgctctgcc gcgaccggtt
     4321 ggtcacggcg gtactcgaga ttgcccagat tggtcagcgc gttggaaagt gtggggtcca
     4381 aggaaagagc tttccgatag gcctgctcgg cctgttcgta cgtctcctca tcctcgtcgc
     4441 aacggcagcc ctcgaggtaa tgcgcatatg cgcgcgatcg gtcgtcgtga tcgaaatgaa
     4501 gcagccgaat gacgtcggac tctaggccat cgatgcgaaa atcgaagacc gcttgacccg
     4561 tgctgggctc gtacttgccc tcctcgtcct gcacgaccac gttcccccca tctcccaaga
     4621 cgcgaagctc ggagaggggg cgtaccgttt ctgggaacga gcgccgcagc gactccaccg
     4681 aaacgcgaac ctgctggagg gaaacgccct ggtcgagaag ggttttcgca acgcgaacct
     4741 cgacgaggtc ctgaaagcgg taatagttac ggcctgcgac gcgagccgag gggccgatca
     4801 gtccggagcg gtgccagtac cgaagacgag aaggcttgta gccgaagagg cgcgcgactt
     4861 cggcgaccgt atacaaacct tggcgcggat cgctccgtgg tggcgctggc tgagtaggag
     4921 ccggctcagg cgtgactgcc cgagtcagct tcccgtcttc accgaaaccg acccgaatca
     4981 cgttgtcccg atcgccgcgt tcgctcaagt ccctctccct gcccgagaat aggccgagct
     5041 tgcctcaacg caagcgtcca tgccgggcgg acgaacccgc cggggcagtg ctacgctccc
     5101 gccatggcgc atccaccagt cgcggccccg atcgcgtttc tctccgatgt tcacgggaat
     5161 ctcgaggccc tggaagccgt cctgtcggag cttgaaagga tgggagtgaa gcgcttgttc
     5221 gtcgctggag accttctcct cgggggagag cagccgctcg aggtctggca gcgattgcag
     5281 gcgctgggtg cgacctgcac acgcggtccg tccgatctgg cgctcggctc cgtcgatcca
     5341 ggaagcctcg tcccaatcga caacgagcag agacgtcaag cccggctgtt cgccgagacc
     5401 cgagccgcca tcggagacct ggtcgccgaa cggctgcgac gacttcccga acagcagcgc
     5461 attccactcg tagacggccg tgaagtcctg atggtccacg gctcaccggc cgacagcagt
     5521 cacgagatcg gtcacgacct cagcgacgac gagatcctct cgctgctcga cgacgaccct
     5581 gccgacatca tcgtctgcgg ctctacgcac gtgccgtttc agcggaccgt cgaggagtat
     5641 cacatcgtga acgtcggctc ggtgggcgcc gcccccgagg ggcgagtggc acactacacc
     5701 gtcctcacgc cacgcatgag cagcgcagac atcctacagg actgggtcag ttacgaagag
     5761 cccgacgcgt cctagacggc agcaaccttg ggctcctggg catcgccggg ctcctcggat
     5821 ttcacgcgga gcatttcgac acgacggaca cggcgttcgt ccgcgtcgag gacgacgaaa
     5881 cggtaaggcc ccgcaaggag agtctcgccg atggcaggca cctgaccgag cagatgcacc
     5941 agcattccgc caagtgaatc gaactcgctc ttgtcttcac agatcgattc gccgagatgc
     6001 tcttcgagat catagactga aatgctcgcg tcgacgaagt agtggcctgg cgcccgctcg
     6061 tagaccagcg gtgcttcttc gtcgtgctcg tcctgaatct ccccgacgat ctcctcgacg
     6121 atgtcttcca aggtgacgat gcccgcggct ccgccaaact cgtcgacgac gacggcaagg
     6181 tgcgagcgtc ggcgctgcat ctcgcgaagg agaacaccga tcttctgact ctcggctgcg
     6241 aagaacacgg gcttccgaag aacatcggcc aaaagaatgt tcgccgcgtc gctgctgtcg
     6301 cgcagcgcct ggaagagatc tttcgcgtag agaatccctt cgacttggtc gatctgaccg
     6361 cggtacaccg ggtaacggct atggcccgac tcgttgatct ggaccgacac ctcgtcgatg
     6421 cccgttttga tgtcgaacgc cacgacctga gtgcgcggca ccatgatttc gcgagccacc
     6481 gtattcttga actcgaggac gctccgaagc atctgagcgt gatcctcggc aatggtcccg
     6541 atctcctcgc cctgctcgat cacgtgctcg acggttagct ccgtcacgcg atgggggtcg
     6601 tcgagctgaa ccatcgggac gaatctctcg atgaggtccc tgatccagtt caagggggca
     6661 gcaaggggtg tgagaagcaa atcgaacggt cggagccact gccacactct gagtgtcgcc
     6721 cggcctgagc gctgccgaat caccgcaatg gcgattgtcc cagccacgac gtaggccaac
     6781 gaaatcccga cgatgccagc gagcgccacc cccaagccgg atgagcgcag caatgaatat
     6841 ccgacgaagg aagccgtgag ggccaaggag agcgctcggc ccaagaagat gcgggcgtga
     6901 accgagtcga ggtcgcgcaa gacgcgcata gccaccctgg cgcttcgatc gtcagcttcg
     6961 gaaagggcat gcagaccggc ttccccgagt gaatgcaatc ccttgccaac cgctgcgagc
     7021 agcgcgccga ggcccacgcc gccgagcatc gcgacggcca actgtgtact gggaacgtcc
     7081 aaagtcctat gcgctctcag gttaccgagg acctcgagtg ctcgcaacgg cagggctcag
     7141 gcatcgcgga gctggtcccg aagcgcccga attcgcaggc gaagtgcgtt gagtcggatg
     7201 aagcccgtcg catcctgctg gtcgtagacc tcatcggctt caaacgtcgc gaattcagga
     7261 tcgtagaggc tattcgggct tcgccggccc acgacacttg cgctgccctt gtagagcttg
     7321 attcgcgcct cacccgtgac gtccttctgt gcctcgtcga tgagcgcctg gagcatttcc
     7381 cgctcgggtg cgaaccagaa gccccgatag accagcgaag cgtactcggg aatcaggccg
     7441 tcgcgaatgc gaagaacttc ccgatcgcag gtgatcgact ctaccgcccg gtgcgccctc
     7501 tgaaggatgg ttccacccgg tgtctcgtac acgcctcggg acttcatccc gacgaagcgg
     7561 ttctcgacga cgtcgacacg gcccacgcca tgcttcgaac cgagcgtgtt gagtctctcg
     7621 attaggaccg ctggtgagag acgctcgccg ttgacactca ccgggtcgcc cttctcgtaa
     7681 ccaatgacga cgtactctgg actgtcgggc gcgtcctgag gatcggtagt ccaccggaac
     7741 atgtctttca gcggctcgtt ccaggtgtct tcgagaatgc cgccttcgta gctgatgtga
     7801 aaaaggttgg cgtccatcga gtatggcttc tcggccgtca cctcgatggg aatcttgcga
     7861 tctttgcagt aggcaatgca gtcgcttcgt gatttgagag tccaggttct ccagggggcg
     7921 atgacctgaa tgcgcgggtc gatcgccatc gcggtcagct cgaagcgaac ctgatcattg
     7981 cccttaccgg tcgctccgtg agaaatcgcg tcggcgcctt cttgtcgagc gacttccatc
     8041 atcgctttgg cgatgacagg gcgcgcgatg gacgttccga ggaggtagtc gccttcatag
     8101 accgcgttcg cgcggagcat cggaaaacag aagtcgcgcg caaactcctc tttgaggtcg
     8161 gcgatgatgc acttcgacgc accggtcgca cgagctttct cgtcgaggcc gctgagctct
     8221 tcgccttgac ccacgtccgc gcaaaacgcg atgacctcgg cgtcgtaggt ctctttgagc
     8281 catgtgagga tgacggatgt gtcgaggccg ccgctatagg cgagcacgat cttcttgggg
     8341 gacgctgcca tggggccgga tggtactgcg ccgccgaccc ggtgcaagcg ggcacgcgct
     8401 caggcgaccc ggatgatggc cttggcctcg aggagctctg taaagacacg aagcgcctcg
     8461 agccgcccca tcttgcagag atcgagcacc tcggcgacgg tggtctggcc gtcgacgagg
     8521 gacagaagaa atgaagcctg ggggtccagt ccgagccatc tcactttcgc ggccggcact
     8581 ccgaggttga acacatagtc gagcgagccg atgcgggtcg tgtacctggt ttcgaggcga
     8641 gcgcgactgt tctctgcgta tcgccgagcg ctctcgtcct ccgggtccaa gccgagttgc
     8701 aactctgccg cgttcagggc agccgcgtaa tttcctaggg caaagtggtc cgcgatatcc
     8761 cggtggatct catggctgct cgactcatcc gatccgcgaa gtgaaatcgg ctccacttcc
     8821 gatgcggacg ggatcaggct tcgccgatct cggtcaatga cccaggcgtc tgcagagggc
     8881 cgactcgtcg gccgctcctc gatccctcga tcgccgagcc cgccggtcaa atcgatcgcg
     8941 ccgtcggaac cgagcacgct cggaaacttg gaatcgctcg aacgcggcct agctgatttc
     9001 attcgtcatg ccgacttgcc gaccacgccc ttgaacatgc gctgcgttcg gccaagcgac
     9061 tccaactcgt ctttgagacc tgcgagatca ccgcgctcga gcaaggtgcg tgcattgtcg
     9121 accacgccct tcgccttttc gatggcatca cggccgaagt cgcttccagc aacgatcgac
     9181 tcgacttcgg ggaacagtcg ctcgatttct gcgatcagcg tctccgcttc ctgtcggatg
     9241 cgctcggaag cctcgtcctc gcggcgcgtc accgcgaagt ctgaagcgtc gaccatcatc
     9301 tcgtcgatct cgtcctcggt gagaccgctg gtggccgtga ccgtgatcga ctgctctttc
     9361 ccggtctcca catccttcgc atggacgctc acgatgccgt ctgcgttgat ctcgaaggtg
     9421 acctcgacct ccacctggcc ggcagcggcc cgtcgcagac cggtcaagat gaactcgccc
     9481 agaagctcgt tgtcctccgc gcgccgagat tcgccctgca agaccagtat cttcattgcc
     9541 gtctgattgt cgcgcacggt cgtgaacggc ttgctgcgcg aggtcggaac ggtcgtgttc
     9601 tgcggaatca gctcttcgaa gtagcccccg acgaccatga tgccaagcgt gtgcggtgtg
     9661 acgtcgagaa gcaccatgtc gagctccgga gagtggtcga cgagcgccgc tccctgaatc
     9721 gacgcaccga gcccgacgac ctcgtccgga tggacgcctt tacaagggtc gcgctgaaag
     9781 aacttgctca cgcgctcttg gaccttcggc atgcgagtca tgccgccaac caagatgatg
     9841 tcttcgatct cgtccttctg gagtccagcc tcgccgaggg tgagctcgca tatttgaacg
     9901 gtacggtcga ccaagtccgc agtcagctct tcgagagtgg ctcgggtgag caaccgctga
     9961 agatgcagcg cttcgttgcg agcgctcgaa atgatgaacg gcaggttgac ctcggtttcg
    10021 gtgacagcgg aaagctcgca cttggccttc tccgcggcat ccttgagcct ctgcagcgcc
    10081 atgcggtctt gccgcaggtc gatcccatgc tcgtgctgga acccttcgac gagccagtcg
    10141 atcacgcgct gatcgaaatc ctcgccaccg aggaacgtgt cgcccgccgt gctgatgacc
    10201 ttgaaaacgc ccgtactcgc gatctccaag atggagatgt cgaacgttcc gcccccgaga
    10261 tcgtagaccg caaccgtacg atcgatgttc ttgccgaacc cgtacgcgag ggccgccgag
    10321 gtgggctcat tgatgatccg gatcacgtcg aggccggcga tcgcacccgc gtccttggtc
    10381 gcttggcgct ggttgtcgtt gaagtatgcg ggcacggtca cgacgacttt gttcacctct
    10441 tctccgaggt agtcctccgc gaccatcttc atttcttgaa ggaccatcgc gctgatctcg
    10501 gggatgctgt agaccttgtc gcgcatctta atgcgacagt ccccgtgcgg cccttccacg
    10561 atgtggaacg gagtggtttg gatcgcgttc ttgacctgag gcgtgtccca tttgcggccg
    10621 atgaggcgct tgcaggcaaa tacggtgttc tcagcgttgg tcacggcctg tcgcttggcg
    10681 atgtggccca ccaaacgctt gccgccttca gtaacggcca ccatcgaggg ggtgaccttg
    10741 tatccccccc gattcgcgat
//
LOCUS       DASRZF010000361         2267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4744941, whole genome shotgun sequence.
ACCESSION   DASRZF010000361 DASRZF010000000
VERSION     DASRZF010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4744941"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            299..2005
                     /locus_tag="VFG22_16140"
     CDS             299..2005
                     /locus_tag="VFG22_16140"
                     /inference="COORDINATES: protein motif:HMM:NF011291.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HET6417828.1"
                     /translation="MSEPKGKTESQENAPNFIHDLVRADIESGKHGGRVVTRFPPEPN
                     GYLHIGHAKSICLNFTTAELAAQGRCHLRFDDTNPEAEDTEYVESIREDVRWLGFDWG
                     SHEYFASDYFERLYDCAADLIKRGLAYVDSQSLEEIREGRGDFYKPGVNSPFRDRRVE
                     DNLALLAKMRAGELDEGAHVVRAKIDMASKDVNLRDPLMYRIRKVTHHRTGDDWCIYP
                     MYDYAHPMSDAFEGITHSVCTLEFAHHNPLYQWFVSHFEFDPIPWQYEFARLNLTYTV
                     MSKRLLKRLVEEGHVQGWDDPRMPTIAGMRRRGYTPAAIRRFAERIGVSRRDSIVDVG
                     LLEHALREDLNATSPRTMAVLRPLKLVIENLPEDEELEIEAPFSPDDESFGSRRLHLT
                     REVFIERDDFMEEPMKKWFRLAPGKEVRLRYGALVTCNEIVKDEAGEIIELRCTWDPD
                     SRGGNAPDGRKVRGTIHWVSAKHAVDAEVRLYDRLFNEENPLDEAHAGDFTARINPDS
                     LEVLKDAKLEPNLGSRGAGESVQFERLGYFVVDTDSTPERPVYNRTIQLKDSWSKLIK
                     KN"
     gene            complement(2015..>2267)
                     /locus_tag="VFG22_16145"
     CDS             complement(2015..>2267)
                     /locus_tag="VFG22_16145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417829.1"
                     /translation="GVGLALGYWSGRLLGCHRYDARAISIEVGIQNSALALVLIFGFF
                     DGLGGMAIIAAWWGVWHLISGLTLATYWSKHPVPIPASS"
BASE COUNT          505 a          678 c          671 g          413 t
ORIGIN      
        1 ctgccaccat gctccgcgcg tcacttccag agacccgcac tgagctgtag cgctggtaaa
       61 ggacgaaccc cacgatgacg gctaagacga cgaggataat tttcgaaatc atgcgtcgaa
      121 ggatagtccc gcccggggag cgcgccagag ctccgaagtt cctcgtccta gatgcggcaa
      181 ggcgatggat cgcatacggc ttcgtcatgc tctgcgcgag gaatcccgcg cttgacattc
      241 gcgatgcgcg actgaggcaa atgcggcgtt tgggcggaaa ccgcgctaga cctgcggcat
      301 gagcgagccg aagggcaaga ccgaaagcca agagaacgcg ccgaatttca ttcacgacct
      361 ggtgagggcc gacatcgagt ccgggaagca cgggggtcgg gtggtgacgc ggttcccccc
      421 cgagccgaat ggctacctcc acataggcca tgcgaaatcg atctgcctga atttcactac
      481 ggcggagctc gctgcgcagg gacgatgtca tcttcgcttc gacgacacga accccgaagc
      541 agaagacacc gagtatgtcg agtcgattcg cgaggacgtg cgctggctag gcttcgactg
      601 gggtagtcac gagtatttcg cttccgatta cttcgagcgt ctttacgatt gtgcagcgga
      661 cttgatcaaa cgcggtctcg cttacgtaga cagtcagagc ctggaagaga tccgcgaggg
      721 ccgcggcgat ttctacaaac ccggcgtcaa cagcccgttt cgagaccgaa gggtcgaaga
      781 caacctcgcc ttgctcgcga agatgcgtgc cggcgagctc gacgaagggg ctcatgtggt
      841 gcgcgccaag atcgacatgg cgtccaagga cgtcaacttg cgcgatcccc tgatgtaccg
      901 gattcggaaa gtcacccatc accggacagg tgacgattgg tgcatttacc cgatgtacga
      961 ctacgcccat ccgatgtcgg atgcgttcga aggaattact cattccgttt gtacgctcga
     1021 gttcgctcac cacaatccgc tctaccaatg gttcgtttcg cacttcgaat tcgatccgat
     1081 tccctggcaa tacgagtttg cgcgcctgaa cctcacctac acggtgatga gcaagcggct
     1141 tctcaagcga ctcgtcgaag aggggcacgt ccagggatgg gacgacccac gaatgcctac
     1201 catcgccgga atgcggcgcc gtggttacac accagcggcg atccgccgtt ttgcggagcg
     1261 aattggagtg tcgcgccgcg acagcatcgt cgacgtcggc ctgctcgagc atgcccttcg
     1321 agaggatctc aacgcgactt cgccacgtac catggccgtg cttcgccctc tcaagctggt
     1381 gatcgaaaat cttccggaag acgaggagct agagatcgaa gcgccgttca gccctgacga
     1441 tgagagcttt ggctcgcgaa ggcttcacct cacgcgcgaa gtgttcatcg agcgggacga
     1501 cttcatggag gaacccatga agaagtggtt ccgcctggcg ccaggcaagg aagtgcgcct
     1561 tcgctatggc gcgctggtga cttgcaatga aatcgtcaaa gacgaagccg gtgagatcat
     1621 cgagcttcgc tgtacctggg atcccgattc ccggggcggg aacgctcccg atggtcgcaa
     1681 agttcgaggc acgatccact gggtgagtgc caagcacgcc gtcgacgcgg aggtgcgcct
     1741 ctacgaccgc ctcttcaacg aagagaaccc actcgacgag gcgcatgcag gcgatttcac
     1801 cgcccggatc aatccggact ccctcgaggt attgaaagac gccaagctgg agccaaacct
     1861 ggggagtcgc ggtgcaggcg aaagcgttca gttcgagcgt ttgggttact tcgtggtcga
     1921 taccgacagc acgccggagc ggcccgtcta caaccgtacc attcagctca aagactcctg
     1981 gtccaaactg atcaagaaga actagccatt tggctcagct cgatgcaggt atcggaaccg
     2041 ggtgcttgga ccagtaggtc gcgagcgtga gccccgaaat caagtgccag acgccccacc
     2101 aggccgcgat gatcgccatg cctccgaggc cgtcgaagaa gccgaagatc agaaccaagg
     2161 cgagcgccga attctgaatt ccgacttcga ttgaaatcgc ccgcgcgtca taacgatgac
     2221 agccgagaag ccttccactc caatagccca gcgcgagccc caccccg
//
LOCUS       DASRZF010000362        19508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1305197, whole genome shotgun sequence.
ACCESSION   DASRZF010000362 DASRZF010000000
VERSION     DASRZF010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 19508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19508
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305197"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VFG22_16150"
     CDS             <1..344
                     /locus_tag="VFG22_16150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417830.1"
                     /translation="KPGGSIKIRVKTYAHSLKLREELLEDIAARKAELQRRIDEKRLE
                     LLRMKHDQLASHRRADLGLERQIHELAEKVNRYRIEAVESEDDAALPGAVSESLIRLG
                     RKTKDGRVVRV"
     gene            356..2257
                     /locus_tag="VFG22_16155"
     CDS             356..2257
                     /locus_tag="VFG22_16155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HET6417831.1"
                     /translation="MADGFEIDIDKELAAFEANERARLGLNDDREQWTDAMIDPFFSA
                     KERATTTILNGGLTMAHDEFVASALKGIGYNTRSMECADVASLRYGKEFGNKAQCNPT
                     YFTVGNLVKELTRLRDEEGMTAQEIIESYVFLTAGACGPCRFGMYVTEYRKALRDAGF
                     DGFRVVLFQQQSGFKQATGEEIGLVLNPQFFMALARGLFAGDVLNVLSYRIRPYEVEE
                     GATDRAIAAVKGEVHEALQQNKSVLRALWRGRKYLQAVEVDRLRAKPKVAIIGEFWAM
                     TTEGDGNYHLQRFVESEGGENDIQTLVNWLLYTLWEMRHDTERRMTLRGTDQSYYGLE
                     GSDVGYKMAGVWLGELALRATFATFSAVAGLKGNKLPDMDEIAKVGHSFYNNELRGGE
                     GHMEVAKLVLNVAKQKANMTLSVKPFGCMPSSGVSDGIQTVVTELHPDAIYCPVETSG
                     DGAVNFQSRVQMYMFKARERARQEHQAALDQYGITEDQAREFIKGTRYAATFYKAPHV
                     VAGTSADLIHEIGPLIGKSRIGRAKVHATRALTRTRGFLRQDLPHAKETFDKMAPYLP
                     SLGRLIYVELKEKIPAPKEAWTSLMRRAVPKSEESAESEVPSNHEYISAPEPREEHDL
                     DTVRLQVVG"
     gene            complement(2281..3864)
                     /locus_tag="VFG22_16160"
     CDS             complement(2281..3864)
                     /locus_tag="VFG22_16160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417832.1"
                     /translation="MRIWFSIMLTVACLWTTLASAQSPDICAPVERLDKYRYLRQLTL
                     DLYGRIPTVEEYERLHEMDDVTDEMIDEMIGSTEFFEQLRSYHRNLLWSFLGDDNLLG
                     QVVEEEGDETFRVWVNREKRDNYRGRDVLCLDMEHTNFDADGRALPMIENYLEGDCAG
                     GEGCRMDGWVEVSPYWAPGATIRICAFDAQPNTDATPNDNGDARTCDQVGPGDPLCGC
                     GPNLRHCTAREDDGNYNAVLQALLEEPARIFEQVISSGEPYMNAFSTRQTAMNGALAH
                     YYRYLSGDNDSELRNAPALPYDAEWQMVERESYHSGILTTLGFLRRFASHRARVNRLY
                     TAFLCDPFEAPAGGLPSATDECSSNPDLSSRCGCATCHEAIEPATTHWGRWREGDDFV
                     YQEDISTFSDNCANCEKGSCSNFCRTFYITRELETTPGSVESELGKFKVLGWRTDEEA
                     SALDAGPSALVSRPEYQQQLASCAVRNFAEHVLGRELSADEKTGWLTDKAASFAAAGH
                     DFLKMVKDVVTDERYRRIE"
     gene            complement(3861..5228)
                     /locus_tag="VFG22_16165"
     CDS             complement(3861..5228)
                     /locus_tag="VFG22_16165"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HET6417833.1"
                     /translation="MSKKHGNQLHSNCGRVDLCGPHLRRRTLLQGVLGGAAMYALAPI
                     RPLFAQETGGHRFVACFLEGGWDVLLGADPRDPSGNYEGIDLGTERLALEFRDPRPVS
                     MGGTEHLWGPTMLALEPHAELATFFRGINMNTVSHETAQSYSHTGFQPAGALARGDSI
                     GTVAAAVHDKGSLLPNAAISVPSFNRDFDNRFSAIRMNDPREIRDLIGPTQNSLSEGI
                     EALLGQALDDAYSCVSPAYSGRLPEDELRISRDRIKRLQDQNVVTRFDFNADTPEMNE
                     MRALYPLDSNPGRSVATAAQLLRTGLSTAVTTRVHGGFDTHGNEWAQNQPQRLVESFN
                     AIGAMISHLRADDPSFDRTTVMVYSEFARTPKINGDGGRDHWFADSVMIFGSTLKKGV
                     FGASNEQDLGLISVNTTTGQPDESGVQLRPEHVMATLVQSLGGDASPYREEPLSAWIV
                     KELTP"
     gene            complement(5238..5873)
                     /locus_tag="VFG22_16170"
     CDS             complement(5238..5873)
                     /locus_tag="VFG22_16170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417834.1"
                     /translation="MMWTKGMATGIAVAIACSSLAFGCSSETAGFDNPRRIIDPEQVP
                     ETKRIRRLTADQFFASLKIATGQQWDDERRFAATLGRPDFAGVTVQGREMSVGFAKLA
                     GDAARQTCRRAVGQDRDTADPASRVILNRVSEVGQLDPFDEQVGENLRYLLLRFHGVL
                     ATDDADRRLRPWFTLLQDGLSATDEARFDEAEDRWAAVCTGLALHPDFLTY"
     gene            complement(5870..7345)
                     /locus_tag="VFG22_16175"
     CDS             complement(5870..7345)
                     /locus_tag="VFG22_16175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417835.1"
                     /translation="MLDLRLSRPWLALLLLFGCGDAQLGNSQAGAATTVSVVASCETL
                     AEGESLMGVSPEGEAWVEGDAGMRQLSPDGSSRPIDARFTRSDELVGWDASSAFVVGD
                     NTLWSATFDGSEPVSLPAELGKPRFVCGDPRNTGGSFLVSTRGLFERRDGAWLRWAIP
                     VELLESMTIRDLQGACSGEEPVMYVEAGRSLWEARYGDVASFRELADLSEMTAKGTDV
                     QVGFVAVRDGALVRFDGGAWIEIPFDEGTVTAMSVADGVLWAGVGAELYRRNRFEKWE
                     RLETATWPFPITRIESYAAGGAWLEKGDQLCHVAHPDTIRVDGVRPYQRLGEGAAMSF
                     GVSAGARAEAALSARLDGQSLEVSGATGRWTVSGVSALQPGWHSLTLDITAPTEGLRR
                     VLKFKVEGAAGGPPPPAPEPTVFWETDIRPIYEASCAVCHGDGGNQTFLGSYEAFSAL
                     GQRALDLVSSGEMPPAASAVEALTPAEVALLETWVQEGMEP"
     gene            complement(7466..8821)
                     /locus_tag="VFG22_16180"
     CDS             complement(7466..8821)
                     /locus_tag="VFG22_16180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417836.1"
                     /translation="MNSRFSLGVLLGALSCLGAAGAQAQEPKVVVMDFAGHGGATARA
                     EVLRGLRDEVVFEKREAARKVLSSRRLELSSVAGRAAIAKELDLDYVVWGRVRGRGAA
                     ARAEIRIAGPQGRQITERDAGPPGQSKGNAKIQKAARAALSKAIQVAPPDRAEPVVAT
                     IIVEEIQVSLADDLPTEKPLAPSEESKPEKKSADPAPEKAKKPSASPEAIGPVFNIMA
                     GAGGRTRQVEINVEDAAGDPGTRTYDSGIFLDIVFRLELRPWARSETKGLRGLALEAD
                     GDFGLGLETQVPGSAAKLDTKTWRVLGQLGYLHRLGKHEIGGLLGIGFDALKLETNGN
                     LPSLEYLFLRVGPAYAYFFVERALFLRVDAGFRYAFSYGALEDTFGEATGFGFDAGLS
                     LGGELDVGFSYRVRVSSEFFKPQFGELSGGLPPGLPAAAQGRDGTDLAINFNVMVGWS
                     F"
     gene            complement(8936..9964)
                     /locus_tag="VFG22_16185"
     CDS             complement(8936..9964)
                     /locus_tag="VFG22_16185"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HET6417837.1"
                     /translation="MSQIKWALTILAAIAVLGCTKKEPPAPAAEKAVAVEQEKPAAVA
                     VEPLKPLPNSVDVNMDKVLLGRSLYHDTALSGDGTISCATCHSLDGGGAEPRKTSIGI
                     NGQVGPINSPTVLNSGYNFVQFWDGRAKDLQEQAAGPVANPGEMGETWENVVEKLKKN
                     PEYVASFSKLYDDGITQNNVTDAIAEYEKSLITPSRFDAYLLGDENAITETEKEGYAT
                     FKEVGCTACHSGINVGGTMFQKLGLVKDYFTDRGTPLTDADLGRFNVSKNPAEKHFFK
                     VPTLRNVELTAPYLHDGSRDTLEETVQIMGKYQLGRDLSEAQIKSIVAFLKSLTGELP
                     SHAKPPAG"
     gene            10022..10708
                     /locus_tag="VFG22_16190"
     CDS             10022..10708
                     /locus_tag="VFG22_16190"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET6417838.1"
                     /translation="MSHRRRIYLLRHGDVSYFEPGQEPGDHDPPLNERGVAQAEALAR
                     LLADAPIDLAVCTGLRRTQQTARLAIGERGLEIGLIEGLSEAKTGSFSDVESAEDMEA
                     LFTRAFDGAEQPGARFLTGETYEELGLRVTAAWQGLLSRDDWSQALVACHGVVNRVIL
                     ALGLGTGPEIYRRIEQDAGCLNVLDIEGQGSQARVAYVRLMNFTPYNATKLGMQETTM
                     ETLWRQFLEG"
     gene            complement(10705..11190)
                     /locus_tag="VFG22_16195"
     CDS             complement(10705..11190)
                     /locus_tag="VFG22_16195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HET6417839.1"
                     /translation="MPVDQDAFREALRGWASGVTVVTSRSGDKVHGMTVSAFSSVSAD
                     PPLVLVCANRSSKTHEIIEEGGVFAVNILAAHQAEVSNVFASSKHEDSRLERVDWVEG
                     STGAPLISEALASLECTVTSSHREGSHTIYVGRVESVLVREVEPLLYYKGGYRSLHCD
                     D"
     gene            11284..12489
                     /locus_tag="VFG22_16200"
     CDS             11284..12489
                     /locus_tag="VFG22_16200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417840.1"
                     /translation="MPQQAFLKAIQSYWDALGQPGSPPELTDSRIDAFVDLLHVTSSA
                     EHGFRLLELLDSPYSGIAVGEKSRPWRLHWAIEVGEVEPFVAPGLDGLIFLADTIADP
                     KGQHRVYTIEDGARGDLEFSDLTHVLHWMTAQVRHAKGEIDDAELEAIQSEASSALDD
                     EWESGPTSGLNIVEELLDTPLVEAWDAISRGQWPLVESDGSSPAVEREDGWQRRLSLW
                     LTRRFLATRALELPEEVGVSDMDAIHRALVDHLIDFEQAIHAGDVPRVIEETAASEDR
                     KLASLAREWIERHDGWRTAASVPAPDEDDPFIDEPPPFQHTPFTRKLMHALSASLDRM
                     VERGELEIDPDRKEALLVELVTAGSDARSAKHMLKKITSALVDSEHVEEIYPSDDKIQ
                     DLLKEDLGG"
     gene            12486..13268
                     /locus_tag="VFG22_16205"
     CDS             12486..13268
                     /locus_tag="VFG22_16205"
                     /inference="COORDINATES: protein motif:HMM:NF016915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417841.1"
                     /translation="MIEPPGTQLWADARWRDGWRLQSRWNAGTHRLLDPAGRIVCRGS
                     LSKCEDALDDACPSPLPAEHLVVLLHGLGRTRRSLARLDRALVHAGFTTARLDYPSTR
                     RTIQEHAATIAALLDHVPTPKKLSFASHSLGGLVVRQLLTYDAPWRSVVERIVMLAPP
                     NQGASLAGLLDKGGILRGILGPAYGQIAEGYAESLPVPDVPVAIFAGDAAGLAGDGLL
                     TVDETRLGGLGEHHVVPAVHTFVMNEPFVIRRAVSFLAGAPN"
     gene            complement(13248..14219)
                     /gene="dgcA"
                     /locus_tag="VFG22_16210"
     CDS             complement(13248..14219)
                     /gene="dgcA"
                     /locus_tag="VFG22_16210"
                     /inference="COORDINATES: protein motif:HMM:NF042940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-D-Glu racemase DgcA"
                     /protein_id="HET6417842.1"
                     /translation="MRAESWPIRGGFRIARGARSAAEVVVLELHDGPHSGHAECVPYA
                     RYGESIESVCAEIEAGCRRLHGTEARAEAHAMPSGAARNAIDCALWELEAKRAGQPVW
                     QLAGLDAEPEPVRTMRTVSVGSVDEMRAAALALDGSRVIKVKVDGGPDLERIEAVHQA
                     VPTASLVVDANEAWSVEQMNAWLPRLPELGVSVLEQPLHAGRDSALDGLPRGVLICAD
                     ESFHDRQSFSSIADRYDMVNIKLDKAGGLSEALYCMRLAQSSGVQVMLGCMVSTSLAI
                     EPALLLAPGADYVDLDGPLLLETDREGALHDRGLGLLRPSPSIWGAG"
     gene            complement(14240..15202)
                     /locus_tag="VFG22_16215"
     CDS             complement(14240..15202)
                     /locus_tag="VFG22_16215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417843.1"
                     /translation="MPETTPSVPPRVFEHTSRKDWGRSVLLMEIPDKRTFLFEDGAER
                     SFRPDYWHKMEIIEVQPNEAVRIDRLARRNQEPAPGSSRKSKAPPKKPDITFEQQVAY
                     FMKLYPVGFEDDSYIKAERGNPEGKGSDKLKNAAIERAEDLLSQKNLDELLGNASYEE
                     IHQMIYDLLTGTKSTTQKAEATRFKNLSEDLREQAAVALRELLYGDRTYPIRFDSFVA
                     ALDVEDGATWPLATLLPALVYPTEHLFVKPTFFKKQALILDVDPKYDTTPNSTTYTQF
                     LETANKTMELLQKAGQRPRDLWDVHVFVCKTLSPKAIKEATGSE"
     gene            15387..17771
                     /locus_tag="VFG22_16220"
     CDS             15387..17771
                     /locus_tag="VFG22_16220"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET6417844.1"
                     /translation="MRRSVWLPLAIALALGASCERAGESEPQPGREAVKAPESNPRLP
                     GFASTDEALQKRLGRALASKGPDYVPRTRHLDENGAPRFTNRLIEQGSPYLLQHAHNP
                     VSWYAWSDEAFERAKRENKPIFLSVGYSTCHWCHVMERESFEDEEIAAFLNRHFIAIK
                     VDREERPDIDSVYMSAVNILTGRGGWPMTVIMTPDKEPFFGGTYFPPRKGARGSGAGL
                     SDILAEMLRVYTNEPQQAVARAQELSQRIEQVASVRAGPGVPSDKMIAIAAQQYATMF
                     DRVDGGFTGAPKFPQPSRLLLLLRYARRTGDDGATAMVATTLDRMAAGGIYDQIGGGF
                     HRYSTDAEWLVPHFEKMLYDNAQLALVYLEAWQLTADSDCARVAREILDYVAREMTSP
                     DGGFYSATDADSSTPQGHEEEGWFFTWTPAELEPLLGADDARIVAAAYGVTERGNFEG
                     RNILHRALSERELASDLGVQRKRVAEVLENARKVLYAARSSRPPPIRDEKIIAAWNGM
                     MISAFAKAGWALGEQSYTQVAERAAKFVLERMRARDGSLVRTYAQGEKGVTSFLDDYA
                     SMIAASLDLYETTGNAAWLERAIALQTEQDARYGDSQVGGYYLTPSDGETLLVREKPT
                     YDSAVPSGNSVAASNLLRLHDLTGQEKWRRKAERLFAMLGFRVTRSPTGYPLLLAALD
                     HYYDTALEVAVIAPTHREEAAPLSDPLRKTFVPNKAFVVLSSDDAVKQRTQVPWLEGK
                     VAIGGKSTAYVCERGRCELPTRDASVFQRQIARRQPYPSFAKEPPPRLPFERSK"
     gene            17771..18394
                     /locus_tag="VFG22_16225"
     CDS             17771..18394
                     /locus_tag="VFG22_16225"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HET6417845.1"
                     /translation="MVKRPEDWVLASHNAGKVDELNRLFADMPVNLKLAAELDLPEPE
                     ESGSTFEANAELKALAAAVATGLPALADDSGVEVHALGGEPGIHTGRWAGEKRDFDIA
                     RRRVRARLEELGPQVGHRATYVCVLCVAWPDGRTRTFRGEALGTLVWPIRGELGHGFE
                     PMFLPDGYAITYGEMTPEHRLRVNARAAAIRKLREELFAPAGSTAGT"
     gene            complement(18378..>19508)
                     /locus_tag="VFG22_16230"
     CDS             complement(18378..>19508)
                     /locus_tag="VFG22_16230"
                     /inference="COORDINATES: protein motif:HMM:NF015376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HET6417846.1"
                     /translation="PKAANVHKTEPRSGNRVGSGSDDDGIDWTPPFDYAAAAAEVEIE
                     AEDEPTPRIAARDPETAVAARSYDKPAKKKTLRRRYRVQSGDTLAEIALRHDTTIADL
                     LEANPGLDANRIHPGQTIDLGAPMPAVVFKLERGDTVLAAASRYGVSSRDLQRWNGDL
                     EPRGARPGTSIRIHTRVPVSPSEAIGPTNRGRLENAVRLPPHRGYTIRNPDRAYGTEE
                     TTRWIMEAIDAVSSKYKYTKVVRIHDISDKNGGKLRDHKSHQNGRDADISYYQEDCPR
                     TGCPFEEVKSSDLDVARQWALLEYWLRNGQAEMIFIDYRLQAKLYRYAKRKGATKAQL
                     EQWMQYPRGRYEAEGIIRHFPNHEDHLHVRFVCAYSDVRCRP"
BASE COUNT         3655 a         6129 c         6034 g         3690 t
ORIGIN      
        1 acaagcctgg cggctcgatc aagattcgtg tcaagacgta tgcgcactcg ctcaagcttc
       61 gtgaggagct tctcgaagac atcgctgcgc gaaaagccga gctccaaagg cgcatcgacg
      121 agaagcgcct cgagctcttg cggatgaagc acgatcagtt ggcctcccat aggcgcgccg
      181 acctggggct cgagcgccag attcacgagc tcgcggaaaa ggtaaatcga tatcgcatcg
      241 aagcggtgga gtccgaagac gacgcagctt tgcccggtgc ggtgtccgaa agtttgattc
      301 gtctcggaag aaagaccaag gacggcagag tcgtccgagt gtgaaggggc tagacatggc
      361 tgatggtttc gaaatcgaca tcgacaaaga gctcgctgca ttcgaggcga acgaacgtgc
      421 ccgcctaggg ctgaacgacg atcgggagca gtggaccgat gcgatgatcg atcccttctt
      481 ttcggccaaa gagcgagcaa ccacgacgat cctcaacggc gggctcacga tggctcacga
      541 cgagttcgtc gcgtctgcgc tcaagggcat cggctacaac acgcgctcga tggagtgcgc
      601 ggatgtcgcg tcgctccgtt acggcaagga gttcgggaac aaggctcagt gcaatcccac
      661 ctacttcacg gtgggcaacc tggtgaagga gctgacccgt ctgcgtgacg aggaggggat
      721 gaccgcgcag gaaatcatcg agagctacgt cttcctcacc gcaggcgcat gtggcccttg
      781 ccgcttcggc atgtacgtca ccgaataccg caaagccctg cgagacgccg gcttcgatgg
      841 attccgcgtg gttctctttc aacagcagtc gggcttcaag caggctacag gcgaagagat
      901 cggtctcgtc ctcaacccgc agttcttcat ggcgctcgcg cgaggtctgt tcgctggcga
      961 cgtgctcaac gttctgagtt atcgcattcg tccctacgag gtcgaagagg gcgccacgga
     1021 tcgagccatc gccgcggtga agggcgaggt ccacgaggcg cttcagcaaa acaagagcgt
     1081 gcttcgtgcc ctctggcgcg gccggaagta ccttcaggcg gtcgaagtcg atcgccttcg
     1141 cgcgaaaccc aaggtcgcga ttatcggtga attctgggcg atgacgaccg agggcgatgg
     1201 caactaccat ctccaacgct tcgtcgaatc cgaaggcggg gagaacgaca tccaaacctt
     1261 ggtgaactgg cttctctaca ccctttggga gatgcgccac gataccgaac ggcgcatgac
     1321 gcttcgggga accgaccaat cgtactacgg actcgaaggc tccgatgtcg gttacaagat
     1381 ggcgggggtt tggctcggcg agctcgccct tcgcgccacg ttcgcgacct tcagcgcggt
     1441 cgctgggctc aagggcaaca agctcccgga catggacgag atcgccaagg tcggccacag
     1501 cttctacaac aacgagctgc gtggcggcga aggtcacatg gaggtcgcca agctcgtcct
     1561 caacgtggcc aagcaaaagg cgaacatgac gctcagcgtg aagcccttcg gatgcatgcc
     1621 gagctccggt gtttcggacg gcattcagac cgtcgtcacc gagcttcatc cagacgcgat
     1681 ctactgtccc gtcgagacca gcggggacgg tgcggtgaat tttcagtcac gcgtgcagat
     1741 gtacatgttc aaggcccgag agcgggcacg ccaagaacac caggcagcgc tcgaccagta
     1801 cgggatcacc gaagaccagg cccgcgagtt catcaagggt actcggtacg cagccacctt
     1861 ctacaaggcc ccgcacgtcg tggcaggaac cagtgcggat ctcattcatg agattggacc
     1921 gctgatcgga aaaagccgca tcggacgcgc caaggtgcat gcgacgcgag ccctcactcg
     1981 aaccagaggc ttcctccgcc aagacctgcc tcacgcgaag gaaaccttcg ataagatggc
     2041 gccctatctg ccgtcgctcg gacgattgat ctacgtcgag ttgaaagaga agattccggc
     2101 accgaaagag gcctggactt ctctcatgag gcgcgcggtg cccaaatccg aggagagcgc
     2161 cgagtccgag gtgccaagca accacgagta catttctgca ccggagcctc gtgaggagca
     2221 cgacctggac actgttcgtc tccaggtcgt aggctgagcg ccgagcaggt ctggttttcg
     2281 ctattcgatg cggcggtagc gctcgtcggt cacgacgtcc ttgaccatct tcagaaagtc
     2341 gtgcccggcg gccgcgaagc tcgcggcctt gtccgtgagc cagccggtct tctcgtctgc
     2401 gctgagctcc cgtccgagaa cgtgctctgc gaagttccgt accgcgcagc tcgcgagctg
     2461 ttgctgatac tccggcctcg acacgagggc gctcggcccg gcgtcgagtg ccgaggcttc
     2521 ttcatcagtg cgccagccga gcactttgaa ctttccgagc tcgctttcga cggaacccgg
     2581 cgtcgtttcg agctcgcggg tgatatagaa ggtccggcaa aagttcgagc aagatccctt
     2641 ttcgcagttc gcgcagttgt cgctgaatgt gctgatgtct tcttggtaga cgaagtcatc
     2701 gccctctctc cagcgacccc agtgcgtggt ggccggctcg atcgcttcgt gacaggtcgc
     2761 gcagccgcag cgtgaagaga gatccggatt gctcgagcac tcgtcggtcg ccgacggcaa
     2821 gccgcctgct ggagcctcga atgggtcaca gaggaacgcg gtgtagagtc ggtttacacg
     2881 tgcccgatgc gaggcgaagc gccggagaaa gcccagcgtc gtcaagatgc cgctgtgata
     2941 gctttcgcgt tcgaccatct gccactccgc atcatacggc agcgccggag cgttcctcag
     3001 ctccgagtcg ttgtcgccag agagatagcg gtagtagtgg gcgagcgcac cattcatcgc
     3061 ggtttgccgc gtagagaaag cgttcatgta cggctctccg gaggagatga cttgctcgaa
     3121 gatgcgggcc ggctcttcca gcaaggcttg gagcaccgca ttgtagttgc cgtcgtcttc
     3181 ccgtgccgtg cagtgacgaa gattgggacc gcagccgcat agcggatcgc ccgggccgac
     3241 ttggtcgcag gtacgcgcgt ctccgttgtc gttcggtgtg gcgtccgtgt ttggctgcgc
     3301 gtcgaacgcg caaatacgaa tcgtggcgcc gggtgcccag tagggcgaaa cctcgaccca
     3361 accgtccata cggcagcctt cccctccggc gcaatcgcct tcgaggtagt tctcgatcat
     3421 gggaagcgca cgcccatcgg cgtcgaagtt cgtgtgctcc atgtccaggc acaagacatc
     3481 gcgacctctg tagttgtcgc gtttctcgcg gttgacccag actcgaaacg tttcatcgcc
     3541 ctcttcttcg accacctgtc cgaggaggtt gtcgtcaccc aggaacgacc acaagaggtt
     3601 ccgatgatag ctgcgaagct gttcgaaaaa ctcggtggat ccaatcatct cgtcgatcat
     3661 ctcgtcggtg acgtcatcca tctcgtggag tcgttcgtac tcttcgacgg taggaatacg
     3721 tccgtagagg tcgagtgtga gctggcgcag atatcggtac ttgtcgagac gctcgactgg
     3781 agcgcaaatg tcgggtgact gagcgctcgc aagcgtcgtc cagaggcaag cgaccgtgag
     3841 catgatcgaa aaccaaatcc tcatggcgtc agctccttta cgatccacgc cgacagcggc
     3901 tcttctcggt acggcgaagc gtctccgccg aggctttgca ccagagtagc catcacgtgc
     3961 tcgggccgga gctgcacccc cgactcgtcc ggctgacctg tcgtggtgtt caccgagatc
     4021 aatcccaagt cctgctcgtt gctggctccg aacacaccct ttttgagagt gctgccgaag
     4081 atcatgacgg agtccgcaaa ccaatgatcc cgtccgccgt cgccgttgat cttgggagtt
     4141 cgagcgaact ccgaatagac catcaccgtc gtgcgatcga agctcgggtc gtctgcacgg
     4201 aggtggctga tcatcgctcc gatcgcgttg aacgactcga ccaggcgctg cggctgattt
     4261 tgcgcccact cgttgccgtg cgtgtcgaag ccgccgtgga ccctggtggt caccgcggtg
     4321 ctcagccccg tccgcaagag ctgcgccgcg gtcgcgacgg agcggcccgg attggagtcg
     4381 agcggataga gcgcacgcat ctcattcatc tccggggtgt ccgcgttgaa atcgaaacgc
     4441 gtgacgacat tctggtcctg caagcgtttg atccggtctc gactgatgcg taattcgtct
     4501 tctggaagcc gcccgctata ggctggcgac acgcacgagt acgcatcgtc gagggcttga
     4561 cccaagagag cttcgatgcc ttcgctgagc gaattctggg tcggcccgat gaggtcgcga
     4621 atttctcggg gatcgttcat tcgaatggcc gagaagcggt tgtcgaagtc tcgattgaag
     4681 ctcggcaccg aaatcgcagc gttcggaagt aggctgccct tgtcatgtac tgcagcggcc
     4741 accgttccga ttgaatctcc gcgcgccagc gcgccggcgg gctggaaacc cgtgtggctg
     4801 taggactgcg cggtctcgtg cgaaaccgtg ttcatgttga ttccgcgaaa gaacgtcgcg
     4861 agctccgcat ggggttcgag cgcgagcatc gtgggacccc agagatgctc cgtcccgccc
     4921 atcgaaaccg gccgtggatc tcggaattcg agagccaggc gttcggtccc gaggtcgatc
     4981 ccttcgtagt tgccggatgg gtcgcgtggg tctgccccga ggagcacatc ccagcctccc
     5041 tccaggaaac aggccacgaa acgatgtccg cccgtttcct gggcgaaaag aggcctgatc
     5101 ggtgcgagcg cgtacatggc cgcgccgccg agcacgcctt gcaagagcgt cctccgacgc
     5161 aagtgaggac cgcagagatc cactctgccg caattcgagt ggagctggtt tccgtgtttc
     5221 ttgctcatgc tggtctctca gtacgtcagg aagtcggggt gaagggccaa gccagtgcag
     5281 accgccgccc agcggtcctc ggcctcgtcg aagcgcgcct cgtccgtggc ggagaggccg
     5341 tcttgcagaa gggtgaacca gggccgaaga cgacgatctg catcgtcggt ggcaaggacg
     5401 ccgtgaaagc gcagcagcag gtagcgcagg ttctcgccta cctgctcgtc gaagggatcg
     5461 agttggccca cctcggagac acggttcagg atgacccgtg acgcggggtc ggcggtatct
     5521 cggtcctgtc cgaccgcacg gcgacaggtc tggcgcgctg catcaccagc gagcttggca
     5581 aagcccaccg acatctcacg tccttggact gtaacccctg cgaaatcggg ccggcccaag
     5641 gtcgccgcga accgccgttc atcatcccat tgctggccgg ttgcaatctt caacgaagca
     5701 aagaactgat cggccgtcag gcgtcggatc cgtttggttt ctggaacctg ctccgggtct
     5761 atgatgcgcc gcggattgtc gaagcctgcg gtttcactgc tacaaccgaa cgcgagcgac
     5821 gagcacgcga ttgcaacggc gatgcccgtc gccataccct tggtccacat cacggttcca
     5881 ttccttcctg cacccaggtt tccaacaagg cgacttcggc tggagtgagc gcttcgacgg
     5941 cactcgcggc cggtggcatc tccccgctcg acacgagatc cagagcgcgc tgtccaagcg
     6001 cgctaaacgc ctcgtagcta ccgaggaacg tctgattgcc gccatcgccg tggcatacgg
     6061 cgcagctcgc ttcgtagatc gggcgaatgt cggtttccca aaacacggtg ggttccggcg
     6121 ccggcggagg tggccctccc gcagcccctt cgaccttgaa cttcaatacg cggcggagtc
     6181 cttccgtagg cgcggtgata tccagagtga gcgagtgcca accgggttga agggcactca
     6241 cccccgagac agtccaacga ccagtagcgc cactcacttc caaggattgc ccatctaggc
     6301 gggcgctcag cgcagcttcg gcgcgggctc cagccgaaac accaaacgac atcgcagccc
     6361 cttccccgag tcgctggtat ggccgaacgc cgtctacgcg aatcgtgtct gggtgggcta
     6421 cgtgacaaag ctgatcgccc ttttcgagcc aggcgccccc ggccgcgtag ctctcgatcc
     6481 gcgtgatcgg aaagggccaa gttgcggtct ccaagcgctc ccatttctcg aaacgattgc
     6541 gacggtagag ctccgctccg acacctgccc agagcacccc atcggccaca ctcatggcgg
     6601 tcacggtgcc ttcatcgaac gggatttcga tccaagcgcc gccatcgaag cgaaccagcg
     6661 cgccatcgcg caccgccacg aaaccgacct gcacgtccgt gcccttcgcg gtcatctcac
     6721 tcaagtcggc aagctcgcgg aaggaagcca catcgccata gcgcgcctcc cagagagatc
     6781 gccccgcctc gacgtacatc accggctctt cgcccgagca ggcgccttgt agatcacgta
     6841 tcgtcatcga ttccaggagc tcgacgggga tcgcccagcg gagccaggct ccatcgcgcc
     6901 gctcgaacag gccgcgcgtg gagaccagaa aggagccgcc ggtgttccgc gggtcgccgc
     6961 agacgaatcg cggctttcca agctcggccg gaagcgaaac gggctcggag ccatcgaagg
     7021 tcgcactcca aagcgtgttg tctccgacga cgaaggccga ggatgcatcc cagccgacga
     7081 gctcgtcgga ccgcgtgaag cgcgcgtcga tgggtctaga ggacccgtct ggcgacagct
     7141 gccgcatgcc agcgtcgcct tccacccagg cttcgccctc agggctcacg cccatcagcg
     7201 attcgccctc tgcgagggtc tcacaggagg cgaccacaga gacggtcgtc gccgcgccag
     7261 cctgactgtt cccgagctgc gcgtcaccgc aaccgaagag caaaagtaag gccagccatg
     7321 gccgggaaag ccgcagatcg agcactcact cagcttactg gccctgaggc gattgtcacc
     7381 ccaagggcgt aagaaagcta gattacagaa ggatttcctc tataaatcac acatgaaagg
     7441 gtgtatttta ccccgatcga ccgtctcaga acgaccagcc gaccatcacg ttgaagttga
     7501 tcgccaggtc ggtgccgtcc ctgccctgcg ctgctgccgg caggccaggc ggtaggccgc
     7561 cgctgagctc tccgaactga ggcttgaaga actcagacga gacccggacc cggtacgaga
     7621 agccgacgtc gagctctcct ccgagcgaca agcctgcatc gaagccaaag ccggtagcct
     7681 cgccgaacgt gtcctcgagc gcaccgtagg agaaggcgta tcgaaacccg gcatcgaccc
     7741 ggagaaagag ggcccgctcg acgaagaagt aggcgtacgc aggcccgacg cgcaggaaca
     7801 ggtactccaa cgacggaagg tttccgttgg tttcgagctt cagggcgtcg aagccgatcc
     7861 cgagcaagcc gccgatctca tgcttgccga gccggtgcaa gtacccgagc tgcccgagca
     7921 cgcgccaggt cttggtatcg agcttcgcgg cggagccagg cacctgcgtt tcgagcccca
     7981 gtccgaagtc accgtcggcc tcgagcgcaa gtccgcggag cccctttgtt tcgcttcgcg
     8041 cccagggccg gagctccagt cgaaacacga tatcgaggaa gataccggaa tcgtaggtac
     8101 gcgtcccggg atcaccagcc gcatcttcga cgttgatctc gacctgacgg gtgcgcccgc
     8161 ccgcgccggc catgatgttg aataccggtc cgatggcctc tggactcgcg ctcggcttct
     8221 tggccttctc gggcgctggg tctgccgact tcttctcggg ttttgactcc tccgagggtg
     8281 ccagaggttt ctccgttggg agatcatcag ccagtgaaac ttgaatctct tccacgatga
     8341 tcgtcgcgac cactggctcg gcacggtcgg gcggtgcgac ctgaatcgcc ttcgacagag
     8401 cggcgcgcgc ggctttctga atctttgcgt tgccctttga ttgtcccggc ggacctgcgt
     8461 cgcgctccgt gatctgcctg ccttgcggcc ctgcgatgcg aatctctgcg cgtgctgcag
     8521 ctccgcgacc acgcacgcgg ccccaaacga cgtagtccaa gtcgagctct ttcgcgatgg
     8581 ccgcgcgacc ggcaacactc gagagctcga gtcgacgcga ggagagcacc ttcctggccg
     8641 cttcccgctt ctcgaagacc acttcgtcgc ggagcccgcg gagaacctcc gcacgtgcag
     8701 tggctccccc atggcctgcg aagtccatga ccacgacttt cggctcttgc gcctgcgcgc
     8761 ctgcagcgcc caggcaagac aaggccccga ggagcacccc caaggagaaa cgtgaattca
     8821 tcccaacgtc acgttagcag cagcatcgag ggtgatgcac agtacttgcg cagcgggaag
     8881 gccccattgc ggcgttcgac cgaggcctcg gcagatgtga aaccgcccgg tcccctcaac
     8941 ccgcaggggg tttggcgtgc gaagggagct ccccggtgag gctcttgaga aacgccacga
     9001 tgctcttgat ctgcgcctcg ctgagatccc gaccgagctg gtacttgccc atgatctgga
     9061 ccgtctcttc cagcgtatcg cgcgaaccat cgtgcaggta gggcgcggtc agctcgacat
     9121 ttcggagcgt gggaaccttg aagaagtgct tctcagcggg attcttgctg acgttgaagc
     9181 gtccgagatc ggcgtccgtg agcggggtac cacggtcggt gaagtagtct ttcacgagcc
     9241 ccaacttctg gaacatcgtg ccaccgacgt tgatcccgct gtgacaggcc gtgcatccca
     9301 cctctttgaa cgtcgcgtag ccttccttct cggtctcggt gatcgcattc tcgtccccca
     9361 agagataggc gtcgaatcgc gacggcgtga tcagcgactt ctcatactcg gcgatcgcat
     9421 cggtcacgtt gttctgcgtg atcccgtcgt catagagctt cgagaatgac gcgacgtact
     9481 ctgggttctt cttgagcttc tcgacgacgt tttcccaagt ctcgcccatc tctccagggt
     9541 tcgcgacggg gccggctgcc tgttcttgca aatctttcgc gcgtccgtcc cagaactgaa
     9601 cgaaattgta ccccgagttc aagaccgtcg gggaattgat cgggccaacc tgcccgttga
     9661 tgccgatcga ggtcttgcga ggctctgcgc cgcccccgtc caaggagtgg caggtcgcac
     9721 aggaaatggt cccgtcgccc gatagcgccg tatcatgata gaggcttcga ccgagcagca
     9781 ccttgtccat gttgacgtca acggagttgg ggagcggctt gagaggctcg accgcaacgg
     9841 ccgctggctt ttcctgttcg acagccacgg ctttttcggc ggccggagca ggaggttctt
     9901 tcttcgtgca acccaagacc gcaatcgcgg caaggatcgt gagcgcccac ttaatctgcg
     9961 acatgggacg gaacctagca gctggggcac gaagaggcac ccgctgctat gatgcgcgcc
    10021 ggtgagtcat cgacgaagaa tctacttgtt gcggcacggt gacgtatcct acttcgagcc
    10081 cgggcaggaa cctggcgacc acgatccacc actcaacgag aggggtgttg ctcaggcaga
    10141 ggccctggcg agactcctgg cggacgcacc gatcgacctt gcggtctgca ccggccttcg
    10201 acgaactcag caaaccgcgc gcctcgcgat cggcgaacgc ggccttgaaa tcggcctcat
    10261 cgagggcctg agcgaagcga aaaccgggag tttctcggac gtggaaagcg ccgaagacat
    10321 ggaagccctg ttcacccggg cgttcgacgg cgccgagcag cccggtgcgc gctttctcac
    10381 tggggagacc tacgaggagc ttgggctccg agtcaccgcc gcttggcagg ggcttttgtc
    10441 gcgcgacgac tggagccagg cgctggtcgc ctgccatggg gtggtcaatc gggtgatcct
    10501 cgcgctcggt ttgggaaccg ggcccgagat ctaccgccgg atcgagcaag acgctggctg
    10561 cctcaatgtc ctggacatcg aaggccaagg aagccaggcg cgagtcgcct acgttaggct
    10621 gatgaacttc acgccctaca acgcgaccaa gctcggcatg caggagacga cgatggagac
    10681 gctttggcgt cagttcctcg aaggctagtc gtcgcaatgc aacgagcggt agcctccttt
    10741 gtagtagagc aagggctcga cctcacggac caacacggat tcgacacgac cgacgtaaat
    10801 cgtgtggctt ccctcacggt gggagctggt gacggtgcat tccaaggaag ccagggcctc
    10861 ggagatgagc ggcgcgccag tcgacccctc gacccagtcg actcgctcca gtcgcgaatc
    10921 ctcgtgcttc gacgatgcga aaacgttgga cacctcggct tggtgagcgg ccaggatgtt
    10981 caccgcgaag acgccgcctt cctcgatgat ctcatgcgtc tttgaggacc gattggcgca
    11041 gacgagcacg aggggagggt cggccgagac gctcgagaaa gccgaaaccg tcatcccgtg
    11101 gaccttgtct ccgctgcggg acgtcaccac cgtgactccg cttgcccagc cccggagggc
    11161 ctctctgaac gcgtcctggt cgactggcat gctcttgctc cctgggtcta ggcaaaaata
    11221 gaacgtgttt caagtcgacg ttgggctttc gcctcgccga atttgggcta gtttccggcc
    11281 cctatgccgc aacaggcctt tctcaaggcg atacagtcgt actgggacgc tctcggtcag
    11341 ccgggctcgc cgcccgagct caccgattct cggatcgatg cgttcgtcga tcttcttcac
    11401 gtgacgtcga gcgcagagca cggctttcgt ttgctcgagc tgctcgactc cccgtactcg
    11461 gggatcgccg tcggcgagaa gtcgcgtcct tggcgcctgc attgggcgat cgaggtcggc
    11521 gaggtcgagc cgttcgtcgc accagggctc gacggcctga tttttttggc cgacaccatt
    11581 gcggacccca aagggcagca ccgcgtgtac acgatcgagg atggtgctcg gggcgatctc
    11641 gagttttcag atctcactca cgtccttcat tggatgacag cgcaggtgcg gcatgcgaag
    11701 ggtgaaatcg acgacgcgga gctcgaggca atccagagcg aagcatcgtc ggcgctcgat
    11761 gatgaatggg agagcggccc gacttcgggt ctcaacatcg tcgaggagtt gctagacacg
    11821 ccgctcgtcg aagcatggga cgcgatttcc cgcggccagt ggcctctggt cgagtcggat
    11881 ggcagcagcc cagcagtcga acgcgaggac ggatggcagc ggcggctctc gctctggctc
    11941 acacgcaggt tcctggctac ccgcgcgcta gagctccccg aggaagtcgg agtctccgac
    12001 atggacgcga ttcatcgcgc attggtcgac cacctgatcg atttcgagca agccatccac
    12061 gccggcgacg tccccagggt gatcgaagaa accgccgcgt ccgaagaccg gaagttggct
    12121 tccctggcgc gtgaatggat cgagcgacac gatggctggc gtactgcggc gagtgtccca
    12181 gcacccgatg aggacgatcc tttcatcgac gaaccgcccc catttcagca tacgccattc
    12241 actcgaaagc tgatgcacgc gctttcggcg tcgctcgacc ggatggtgga gcggggcgag
    12301 ctagagatcg accccgaccg caaagaggct ttgctggtcg agctggtcac cgccggttcc
    12361 gatgcgcgct cggccaaaca catgctgaag aagatcactt ccgccctcgt cgattcggag
    12421 catgtggaag agatttaccc gagcgacgac aaaatccagg atttgctgaa agaagacctt
    12481 ggcggatgat cgagccgccg ggcacgcagc tctgggccga tgcgcgctgg cgtgacggct
    12541 ggcgcctgca aagccgatgg aacgccggta cccatcgctt gctcgatccc gcgggtcgga
    12601 tcgtctgtcg tggatcgctt tccaagtgcg aggatgccct cgatgatgcg tgtcccagtc
    12661 cgttgccggc cgagcatctc gtagtcctgt tgcacgggct gggccgcacc cgtcgttcac
    12721 tcgctcgctt ggaccgggcc ctcgtgcacg ccggattcac tacggcccgg ctcgactacc
    12781 cgagtacgcg aaggaccatt caagagcacg ccgccaccat cgctgcgctg ctcgatcacg
    12841 ttccgacccc caagaagctc tcgttcgctt cccatagcct cggcggactg gtcgtccgac
    12901 aactgctgac ctacgacgca ccttggcgct cggtcgtcga gcgaatcgtg atgctggcgc
    12961 cgccgaatca gggcgcctct cttgcgggcc tgctcgacaa aggcggcatt ctgcggggca
    13021 tcttgggacc ggcgtacggc cagattgcag aagggtacgc ggagtcgctt ccggtgccgg
    13081 atgtgcctgt tgcgatcttt gcgggcgatg ctgcaggcct tgccggggac ggcttgctaa
    13141 ccgtcgacga gacgcggctc ggagggctcg gtgagcacca cgtcgtaccc gcggtgcaca
    13201 ctttcgtgat gaacgagccc ttcgtcattc gaagagcggt gtcgtttcta gccggcgccc
    13261 caaattgaag gcgacggccg caggagaccc aagccccgat cgtggagcgc gccttcccgg
    13321 tcggtttcca gcagcaaggg cccatccagg tcgacgtaat ctgcgcccgg cgccaagagc
    13381 agggcaggct cgatcgcaag cgaggtgctc accatgcagc ccagcatcac ctggacgccc
    13441 gagctttgtg ccagcctcat gcagtagagc gcctcgctga gtccgcccgc cttgtcgagc
    13501 ttgatgttca ccatgtcgta acgatcagcg atgctcgaaa acgattgacg atcatgaaac
    13561 gattcatcgg cgcaaatcaa aacaccgcgc ggcagcccgt cgagggcgga atcgcggccc
    13621 gcatgaaggg gctgctcgag gacgctgaca ccgagctcgg gcagcctcgg taaccaggca
    13681 ttcatttgct cgaccgacca ggcttcgttt gcgtcgacga ccaagctggc ggtcggcacg
    13741 gcctgatgca ccgcttcgat gcgttccaag tccgggcctc cgtccacctt gaccttgatc
    13801 actcgagacc cgtccaacgc caaggcggcc gcccgcatct catcgacgct gccgacactc
    13861 acggtgcgca tcgtgcgcac cggctcgggt tccgcatcga gaccggccaa ttgccaaacc
    13921 ggttgcccgg cccgcttcgc ctctagctcc cagagcgcgc aatcgatcgc gtttcgtgcc
    13981 gccccagagg gcatcgcatg tgcttcagca cgggcttcgg tgccgtgaag ccggcggcag
    14041 cctgcctcga tttcggcgca gaccgattct atcgactcgc catagcgggc gtagggcaca
    14101 cattccgcgt gcccactgtg tgggccgtca tggagctcga gcaccacgac ctcggccgcg
    14161 gagcgagcgc cgcgagcgat tcgaaagcct cctcgaatcg gccaggactc agctcgcacc
    14221 gaagccttca tcgcaggggc tactcgctgc cggtagcttc tttgatcgcc ttggggctga
    14281 gcgtcttgca gacgaatacg tgaacgtccc agaggtcgcg tgggcgctgc ccggccttct
    14341 ggagcagctc catcgtcttg ttcgctgtct cgaggaactg cgtgtatgtt gtcgagttcg
    14401 gtgtcgtgtc gtatttggga tcgacgtcca agatcagcgc ctgtttcttg aagaaggttg
    14461 gcttcacgaa caggtgttcc gtcgggtaga ccagtgccgg gagaagggtc gcaagcggcc
    14521 aggtcgcgcc gtcttccaca tcgagcgcag ccacgaacga atcgaatcga atcgggtatg
    14581 tcctgtcgcc gtagagcagc tcacggagcg caacggcggc ctgttcgcgc aagtcttcag
    14641 agaggttctt gaagcgagtg gcctcggcct tctgcgtggt gctcttggta cccgtgagaa
    14701 ggtcgtaaat catctgatgg atttcttcgt agctcgcgtt gccgagcaac tcgtcgaggt
    14761 tcttctggct cagaagatcc tcggcgcgct cgatggcggc gttcttgagc ttgtcgctac
    14821 ccttgccttc ggggttgcct cgttcggcct tgatgtagga atcgtcctcg aagccgacgg
    14881 gatagagctt catgaagtaa gccacctgct gctcgaaggt gatatcgggc ttctttggtg
    14941 gggccttgga cttccgcgag gacccggggg cgggttcctg attgcgccgg gcgagtcgat
    15001 cgatgcgaac cgcctcgttc ggctgaactt cgatgatctc catcttgtgc cagtagtccg
    15061 gccggaagct gcgctcagcg ccgtcttcga agaggaacgt gcgcttgtcg gggatctcca
    15121 tgaggagcac tgagcgcccc cagtccttgc ggctggtgtg ctcgaaaacg cggggcggta
    15181 cggagggtgt agtctctggc atcggttaca gatcctggaa aatcgggtag aagccggccc
    15241 cgggggccag aaaatcgagc ccgtcgtgta tcacggacat gcggcccttg catccgcttg
    15301 cgatcgtccg ggtgtagccc tacgagccct gctcggcaac ctcccgttct cattggggtt
    15361 tgggcagaat gaaccaattt tcagtaatgc gccgctccgt ttggcttccg cttgcgatcg
    15421 cgcttgctct cggtgcctcg tgcgaacggg ccggcgagag cgagcctcag cccggccgcg
    15481 aagcggtcaa ggcgccggag agcaacccga ggctgccggg cttcgcctcc accgacgagg
    15541 ctcttcagaa gcggcttgga cgcgcgctcg catcgaaagg gccggactac gtcccacgta
    15601 cgcggcacct cgatgagaac ggcgccccca ggttcaccaa tcgtttgatc gagcaaggaa
    15661 gcccgtatct tcttcaacac gcgcacaatc cggtgagttg gtacgcctgg tccgatgaag
    15721 ccttcgagcg cgccaagcgc gagaacaagc cgatcttctt gtcggttggc tactcgacct
    15781 gccactggtg ccacgtgatg gagcgcgaat ccttcgagga tgaggagatc gccgcgtttc
    15841 tcaaccgcca cttcatcgcg atcaaggtcg acagggaaga acggcccgac atcgacagcg
    15901 tatacatgag cgccgtcaac atcctcacgg gccgcggcgg ttggcccatg acggtcatca
    15961 tgacaccgga caaagagcca ttctttggag gcacgtactt cccgccccgc aagggagcgc
    16021 gcgggagcgg cgcgggcctc agcgacatac tcgcagagat gctgcgagtc tacaccaacg
    16081 agccccagca agccgtggca cgggctcagg agctgagcca gcgcatcgag caagtcgcct
    16141 cagtgcgagc agggcccgga gtgccgagcg acaagatgat cgcgatcgcg gcgcagcagt
    16201 acgcgacgat gttcgatcga gttgatggcg gcttcacggg ggcccccaag ttcccacagc
    16261 cctcacgtct cttactgctt ctgcgatatg ctcgtcgcac gggcgacgat ggagccacgg
    16321 ccatggtggc cacgacactc gaccgcatgg cagctggcgg catctacgat cagatcggcg
    16381 gcggcttcca ccgttactcg acggatgctg agtggctcgt tccgcatttc gaaaagatgc
    16441 tctacgacaa cgctcaactg gccctcgtct atctggaggc ctggcaactc acggccgact
    16501 ctgattgtgc tcgcgtcgcc cgcgagattc tcgactacgt cgcacgggag atgacctcgc
    16561 ccgatggcgg cttttactcg gcaaccgacg ccgatagctc gacgccccag gggcacgagg
    16621 aggagggttg gttcttcacc tggacgcctg ccgagctcga gccactgctc ggcgccgacg
    16681 acgcgcggat cgtcgctgcc gcatacggcg tgacggaacg agggaacttc gaaggacgca
    16741 acattctcca ccgagctctg tccgagcgcg aattggcctc ggacctcggg gtgcagcgca
    16801 agcgcgttgc cgaggtactg gagaacgccc ggaaggttct ctacgcagcg cgtagctcac
    16861 ggccgcctcc catccgcgat gagaagatca tcgcggcctg gaacggaatg atgatcagcg
    16921 cattcgccaa agccggctgg gcgctcggcg aacagtcata cacacaagtc gccgagcgcg
    16981 cggcgaagtt cgtgctcgaa cgaatgcgcg ctcgagacgg ctcgctggtc cgcacctatg
    17041 cccagggaga gaagggcgtg acgtcgtttc tagacgacta cgcttcaatg atcgcagcaa
    17101 gcctcgatct ctacgagacg acgggcaacg cggcttggct cgagcgcgcc atcgcgcttc
    17161 aaaccgaaca ggacgcacgc tacggcgact cgcaagttgg tggctattac ctcacgccga
    17221 gcgatggaga gacactgctg gtgcgggaaa agccgaccta tgatagcgcc gttccctcgg
    17281 ggaattccgt ggcggcgagc aacctcctcc gcctccacga tctgacgggg caggagaaat
    17341 ggaggcggaa ggccgagcgc ctcttcgcca tgctgggatt ccgggtcact cgctcgccta
    17401 cgggataccc tttgttgctg gccgcactcg accactacta cgacacagcg ctcgaggttg
    17461 cggtgatcgc cccgacgcat cgcgaggagg ctgcaccgct ttccgatccg cttcgcaaga
    17521 ccttcgtccc caacaaggcg ttcgtggtcc tttcaagtga cgacgcggtg aaacaaagga
    17581 cccaggtccc ctggctcgag ggcaaggtcg ctatcggcgg gaagagcacg gcatacgttt
    17641 gcgagcgggg caggtgtgag ctcccaaccc gcgatgcgtc cgtgtttcag cgacagatcg
    17701 cgcgacgcca gccgtacccg agctttgcga aggaacctcc accccgccta ccgttcgaac
    17761 ggtcgaagtg atggtcaaac gcccggaaga ttgggtcctc gcgagtcaca acgccggcaa
    17821 ggtcgatgag ctcaatcggc tcttcgccga catgcccgtc aatctcaagc tcgcagcgga
    17881 gctcgactta ccagagccag aagaaagcgg atcgacgttc gaagccaatg cagagctcaa
    17941 ggcactggcg gctgccgtgg ccacggggct ccccgcattg gcggacgact caggcgtcga
    18001 agttcatgcg ctcggcggcg agccaggtat tcacaccggc cgctgggcgg gcgagaaacg
    18061 cgacttcgat attgcacgcc gccgcgttcg agcacgcctc gaggagctgg gtccacaggt
    18121 tggccatcgc gcaacttacg tctgcgttct atgcgttgcc tggcccgacg ggcgcacgcg
    18181 cacgtttcga ggcgaagcgc tcggcacgct ggtctggccg atccggggtg agttggggca
    18241 cggcttcgag ccgatgttcc tgcccgacgg ttacgcaatc acgtacggcg aaatgacgcc
    18301 cgagcatcgg cttcgcgtca atgcacgcgc cgctgcgatc cgaaagctca gagaagagct
    18361 ctttgctcct gcgggttcta cggccggcac ctgacgtccg aatacgcgca cacgaaccga
    18421 acgtgaagat ggtcctcgtg atttgggaaa tgtcgaatga tcccctctgc ttcatatcgg
    18481 ccgcggggat attgcatcca ttgctcgagt tgggccttgg tcgccccttt gcgcttcgcg
    18541 tagcgatata gctttgcctg caaacgatag tcgatgaaga tcatctcggc ctgtccgttg
    18601 cgaagccaat attcgagcag ggcccactgc cgcgcgacgt cgagatcgga cgatttgact
    18661 tcctcgaagg ggcagccggt tcgagggcaa tcctcctggt agtagctgat gtcggcatcg
    18721 cgtccgtttt gatggctctt gtggtcgcgc agcttaccgc cgttcttgtc actgatgtcg
    18781 tgaatgcgga ccactttcgt gtacttgtac ttcgagctca ccgcgtcgat cgcttccatg
    18841 atccagcggg tagtctcctc ggtgccgtat gcccgatcgg gatttcgaat cgtatacccc
    18901 cgatgcggag gcaggcggac cgcattttcg aggcgcccgc ggttggtcgg tccgatcgcc
    18961 tccgagggac tcaccggaac tcgcgtgtgg attcgaatgc tcgtgccggg acgtgcacct
    19021 cggggctcta ggtccccgtt ccagcgttga agatctcgcg agctaacgcc gtagcgactc
    19081 gcagctgcta ggacggtgtc cccacgctcg agcttgaaga ccaccgctgg catcggcgct
    19141 cccaagtcga tcgtctggcc cgggtggatt cggttcgcgt ccaatccggg gttggcctcg
    19201 agcagatccg caattgtcgt gtcgtgcctc agagcgattt ctgcaagcgt gtcgccgctc
    19261 tgcacacggt agcgtctacg gagggtcttc ttctttgccg gtttgtcgta actgcgtgcg
    19321 gcgaccgcag tttcggggtc gcgggctgca attcgcggag tgggctcatc ctccgcctcg
    19381 atctcgactt ccgcagccgc cgctgcgtag tcgaacggag gcgtccaatc gatgccgtcg
    19441 tcatcggatc cagaacctac ccgattcccc gagcgcggtt cggtcttgtg cacgttcgcg
    19501 gcctttgg
//
LOCUS       DASRZF010000363         5110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_968073, whole genome shotgun sequence.
ACCESSION   DASRZF010000363 DASRZF010000000
VERSION     DASRZF010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5110
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968073"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VFG22_16235"
     CDS             <1..258
                     /locus_tag="VFG22_16235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417847.1"
                     /translation="VVALLLSAWVTYKFFGVFIPVLTWLRAALAGLSGYWLAAAVPHG
                     SAWMTIVALLLGFVSAVIVLVITRELGNDDWQALRRVARRD"
     gene            complement(255..743)
                     /gene="moaC"
                     /locus_tag="VFG22_16240"
     CDS             complement(255..743)
                     /gene="moaC"
                     /locus_tag="VFG22_16240"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HET6417848.1"
                     /translation="MNENEELTHLDRDGQARMVDVGEKDATQREARARASLRMNPETR
                     RVLLTGQVPKGDALAAARIAGIQAAKRTSELIPLCHPLAITGCDVAFEPADDEERFVI
                     ETSVRCTGRTGVEMEALTAAAVAALTLYDMLKAIDREMAIEEIVLVEKQGGRSGHYRR
                     GR"
     gene            complement(743..1357)
                     /locus_tag="VFG22_16245"
     CDS             complement(743..1357)
                     /locus_tag="VFG22_16245"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET6417849.1"
                     /translation="MTSSERLMEIAERLAELDPVRTYAQRGAALIAGLVGAHSFRLER
                     PDADTLSSESALITSEPTISMPLRQGRTNIGTLHLEIVGDAPNGNQLELLKWVSRLYA
                     RGLGYTERLSDGGSRRPGEAVEQTLRRAPLTPRERDVVSFLVGGSSTRDIASETGLTV
                     STVNTYLKRIFSKLGVHSRVELIARMAGTATIPPRPNHDGTLAD"
     gene            complement(1413..2312)
                     /gene="gluQRS"
                     /locus_tag="VFG22_16250"
     CDS             complement(1413..2312)
                     /gene="gluQRS"
                     /locus_tag="VFG22_16250"
                     /inference="COORDINATES: protein motif:HMM:NF012951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HET6417850.1"
                     /translation="MASTYRTRFAPSPTGKMHPGHARTALVTWLQARKQGGRIVMRIE
                     DIDRPRVVEGSADEICRDHEWLGLDWDEGPVFQSDHGEAYERALAQLEDAGLVYPCTC
                     SRREIAAAASAPHGDDGPRYPGTCRNAVARPGKPNALRFRFEDPSPGFCDALCGPYPE
                     GLAGGDFVLRRADGIWAYQLAVVVDDANANISQVIRGDDLLASTPRQIALYRALDRPV
                     PSFAHVGLVLDAGGQRMATRDGATSIASLRRAGRSAQQLIGELACSLGLTESNAPIRA
                     EELISSFELSKIQRSPFTWKPRG"
     gene            2302..3126
                     /locus_tag="VFG22_16255"
     CDS             2302..3126
                     /locus_tag="VFG22_16255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HET6417851.1"
                     /translation="MEATAHPLYDLRRGGHAVHLPTVRGRTDVCGCFPTGRHYPGIAD
                     LIAQAIQNVEGLSHEFDPGHSWEDIPITVIDFETTGRDPETDRVIEIGLVTFEKGRVS
                     SRESWLVNPGIPVPEESRAVHGITDEELQGAPDFATVMPRLLEHLRGKLPAAYNAPFD
                     RGFLHAEIARVAPEGMTPGDLPPAAREDVVWVDPLVWARELLKELKTRRLGDVAKHLS
                     IPLEQAHRAAGDAEATGRILLALAPQLPRVYGELVRLQKRYAAFQDAEFAAWKRFR"
     gene            3123..4022
                     /locus_tag="VFG22_16260"
     CDS             3123..4022
                     /locus_tag="VFG22_16260"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HET6417852.1"
                     /translation="MSPEITKARLIWFPFALMAVGSWTPVLGMWLNGIEWSGAAGRAV
                     AFWYTVPALIAILLVQGPWLKKPILEPLGIRFSINRWWGIGWLLPVAILGIGMWVTWA
                     AGFDPVLTVQQLIENKRSTVPPDQLAAFDTYLAESPPPHPLMLIVMGMPAGLTFNLIP
                     GLCEEIAFRGYYFREISGGFWARSTKIGLLWWLWLAPSVAIGNLYGVPSVWGVALALP
                     WCIVASWVLVYLRVRSESVIAVGVARGTLLALTAAAHDLTFGAPTWLAPFYGIGGLVG
                     LLVVWLAFWIHDRSRAKGRLTTA"
     gene            complement(4243..4374)
                     /locus_tag="VFG22_16265"
     CDS             complement(4243..4374)
                     /locus_tag="VFG22_16265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417853.1"
                     /translation="MIVERDLEERIEQRDARVVHEKSNLVPFRPASGLVLQVRFREI"
     gene            4436..4615
                     /locus_tag="VFG22_16270"
     CDS             4436..4615
                     /locus_tag="VFG22_16270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417854.1"
                     /translation="MVRDKEFGPEFNTFRGEGPWNPLLVARAVLRGLGMSGDDAKAAL
                     RPDQWEAWAYQRGED"
     gene            complement(4718..4791)
                     /locus_tag="VFG22_16275"
     tRNA            complement(4718..4791)
                     /locus_tag="VFG22_16275"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4755..4757),aa:Asp,seq:gtc)
BASE COUNT          887 a         1598 c         1624 g         1001 t
ORIGIN      
        1 gtcgtcgcgc tgcttctctc ggcgtgggtg acctacaagt tcttcggagt gttcatcccc
       61 gtgctgacct ggttgcgtgc agcattggcg gggctgagcg ggtactggct cgcagccgcc
      121 gttccacacg gttcggcatg gatgaccatc gttgccttgc ttctcggctt cgtgagcgcc
      181 gtcattgttt tggtgattac ccgcgagctc gggaacgacg actggcaagc gcttcggcgc
      241 gtagcgcgcc gggactagcg tcctcggcga tagtgaccgc ttcgcccgcc ctgcttctcc
      301 accaacacga tctcttcgat cgccatctcg cgatcaatcg ccttcagcat gtcgtagagc
      361 gtgagggccg cgacggccgc ggcggtcagc gcttccatct cgacccccgt gcggcccgtg
      421 caccgaaccg atgtttcaat cacgaatcgc tcctcatcgt cggctggctc gaaagccacg
      481 tcgcaaccgg tgatggcgag gggatgacaa agtggaatca gctcggaggt ccgcttggct
      541 gcctgaatgc ccgcaatgcg cgcggcagcg agcgcgtcgc ccttggggac ctgaccagtc
      601 aagagaacgc gccgcgtttc tggattcatg cggagggagg cacgcgcacg ggcttcgcgt
      661 tgggtcgcgt ccttctcccc gacgtcgacc atccgggcct gaccgtcgcg atccaaatgt
      721 gtgagctcct cgttctcgtt catcaatcgg cgagcgtccc gtcgtggttg ggccgcggcg
      781 ggatggtagc tgtcccggcc atcctggcaa tgagctcgac cctcgaatgc acaccgagct
      841 tcgaaaaaat gcgtttgagg taggtgttga cggtggacac ggtgagtcct gtctccgacg
      901 cgatgtcgcg ggtgctcgaa cctccgacca agaacgagac gacatcgcgc tcacgcggag
      961 tgaggggggc gcgacgcaga gtctgctcga cggcctcacc cggccggcgg ctgccgccat
     1021 cgctgagtct ttccgtatag cccagtcctc tcgcatagag tcgcgagacc catttcagca
     1081 gctcgagttg gtttccattg ggagcgtctc cgacgatttc caagtgcagc gttccaatgt
     1141 tcgtgcgccc ctggcgaaga ggcatcgaaa tcgtcggctc cgaagtgatt agggcggact
     1201 cactgctgag agtgtcggca tcgggccgct cgagtcgaaa cgaatgggcc ccgaccaggc
     1261 ccgcgatcaa tgcggcgcca cgctgcgcgt acgtcctgac cgggtcgagc tccgctaggc
     1321 gctcagcgat ctccatcagc ctttcgctcg atgtcactgc ccgttcctca agtcacttga
     1381 gactcaaatc agggtggaaa accgaattaa tgtcaacccc ggggtttcca ggtgaacggc
     1441 gaccgttgaa tcttgctcag ctcgaagctc gaaatcaact cctcggcgcg aattggagca
     1501 ttgctttcgg tgagtccgag ggagcaggcg agctctccga tcagctgttg ggccgaacgg
     1561 ccggctcttc gcagggaggc gatcgacgtt gccccatccc gtgtggccat tcgctgaccg
     1621 ccagcgtcca gcaccaatcc tacatgtgcg aagctcggca cgggccgatc gagcgcacgg
     1681 tagagtgcga tctggcgcgg cgtggaagcg agtagatcgt caccccggat cacttgactg
     1741 atgttcgcat tcgcgtcgtc gacgaccacc gccagttggt acgcccagat gccatcggcg
     1801 cgccggagga cgaagtcacc gcccgcaagt ccctcgggat acggaccgca aagcgcatcg
     1861 caaaaacccg gcgagggatc ctcgaatcga aagcgcagag cattgggctt gccaggccga
     1921 gcgacggcgt tgcggcaagt gcctggatac cgtggaccgt catctccatg cggggcgctt
     1981 gcggcggccg cgatctctct ccgagagcag gtgcaagggt agaccagtcc ggcgtcctcg
     2041 agctgggcaa gcgctcgttc gtacgcttcg ccgtgatcgg actggaacac tgggccttcg
     2101 tcccagtcga gacctagcca ttcgtggtcg cggcatatct catcggcgga cccttccacg
     2161 acccgcggtc tgtcgatgtc ctcgattcgc atcacaatcc ggccgccttg cttgcgggct
     2221 tgcagccacg tcacgagggc cgtgcgcgcg tgcccggggt gcatcttgcc ggtcggtgaa
     2281 ggtgcgaagc gggttcgata cgtggaagcc acagcccatc ctttatacga cttgcgccgg
     2341 ggcggccatg cagtgcatct tccgaccgtg cggggacgaa ccgatgtgtg cggctgcttt
     2401 ccgactggac gccactatcc agggattgcg gacctgatcg ctcaagcgat tcagaacgtc
     2461 gaaggcctca gccatgagtt cgatccgggt cactcttggg aggatattcc gatcacggtg
     2521 atcgatttcg aaacgaccgg tcgggatccc gaaaccgacc gggtcatcga gatcgggctc
     2581 gtcactttcg aaaagggtcg cgtcagcagc cgcgagagct ggctggtcaa tcctggaatt
     2641 ccggtgcccg aggagtcccg tgcagtacac ggcatcacgg acgaagagct tcagggcgcg
     2701 cctgatttcg cgaccgtgat gccacgactg ctcgagcatc tgcgaggaaa gttgccagcc
     2761 gcatacaacg caccgttcga ccgtgggttc ttgcacgcgg agatcgcgcg cgtcgcgccc
     2821 gagggaatga cacctggcga cctgccgcca gccgctcgcg aagacgtggt ctgggtggat
     2881 ccgctggtct gggcgcgtga gctcctcaag gagctgaaga cccggcgcct tggcgacgtg
     2941 gcgaagcatc tgagcatccc tctcgagcag gcccaccgcg ccgcagggga tgccgaggcg
     3001 actgggcgca tcctgctcgc gcttgcgccc cagttgccgc gagtctacgg cgagctcgtt
     3061 cgcttgcaga agcgatacgc cgcatttcag gacgccgagt ttgctgcctg gaaacgcttt
     3121 cggtgagccc ggagatcacc aaggcacgcc tgatttggtt tccgttcgcc ctgatggccg
     3181 tcgggagctg gacaccggtg ctcggcatgt ggctgaacgg catcgaatgg tcgggagcgg
     3241 caggccgggc cgtcgcgttt tggtacacgg ttcccgcgct catcgcgatt cttctggtcc
     3301 agggaccctg gctgaagaag cctattctag agccgcttgg gattcggttc tcgatcaatc
     3361 gctggtgggg catcggctgg ctcttgccgg tggcgatcct cgggatcggc atgtgggtca
     3421 cgtgggctgc gggcttcgat cccgtgctca cggttcagca actcatcgag aacaagcgct
     3481 cgacggtgcc gcccgatcag ctcgccgcgt tcgacacata cctcgccgaa agccctccgc
     3541 cgcatccact gatgctgatc gtgatgggaa tgcctgcggg cctcaccttc aatctcattc
     3601 cggggctctg cgaggagatc gcgtttcgcg gctattactt tcgggagatt tcgggtggct
     3661 tctgggctcg atcgacgaag atcggtctcc tctggtggct ctggctcgct ccatcggtgg
     3721 ccattggaaa cctctacggc gtaccgagcg tctggggagt tgccctagcc ctcccctggt
     3781 gcatcgtcgc ctcctgggtg ctggtctatc tgcgtgttcg aagtgaatcc gtcatcgcgg
     3841 tcggcgtagc gcggggcaca cttctcgctc tgactgcggc cgcgcatgac ctcaccttcg
     3901 gagcacccac ctggctcgcg ccgttctacg gaattggagg gctcgttggg ctcctcgtcg
     3961 tttggcttgc tttctggatc cacgaccgca gtcgtgcaaa aggtcgattg accacggcct
     4021 gaggagatcg acttggcaga caacacgagg ctcgggttga aagctcgtta cggaggttgt
     4081 gcgctcgtca ctggcgcggc gcggggaata ggacgcgcgt tcgcccagct tctcgcggct
     4141 gaaggcttcg atctcttgct cgtggaccgg gaagaggcgg aaacgaaggc gctcgccgac
     4201 gaacttcacg gaacctacgg aatcgaggcc acagcccaga tctcagatct cgcgaaaccg
     4261 gacctggaga acgaggcccg aagctgggcg gaacggtacg agattggact tctcgtgaac
     4321 aacgcgggca tctcgttgct cgatccgttc ttcgagatct cgctcgacga tcacctcagc
     4381 accttggact tgaactgtcg ggcaacgctc gtgctcacgc acatcatcgg ccgccgtggt
     4441 aagggacaag gaattcgggc cggagttcaa cacgttccgc ggagaggggc cttggaaccc
     4501 gttgctcgtc gcacgcgctg tcttgcgcgg ccttggtatg agcggggacg acgcgaaggc
     4561 cgctcttcgt ccggaccaat gggaagcctg ggcctaccag cgcggcgagg actaggcggc
     4621 tggtccagcg tgtcacgagg ccgcgaaaac taccggcata ggtaccggca cccgaaacga
     4681 aaaaacccgg gagatcattt tctcccgggt tttccagcgc ggcggacggg actcgaaccc
     4741 gcggcctccg gcgtgacagg ccggcgttat aaccaactta actaccgccg catccacctc
     4801 agggcatcgc cctgcgggac cggccttgta gcctgggggt tgtgggctgt caaagagtgg
     4861 aagcgccttt cgctgcaacg cgactcacct gcaatttcgc gcaagctcga gcgctctgct
     4921 tcgttgcttt cggccgccgt tagagaagcc aaaccgacgc ggttctcgtc actgagcccg
     4981 tcgcgcggga gcccgtccta cacccttgca agacgtgatg tgcacgttgc ttgccagaag
     5041 gcgatcgcta cgccagggct cgaacgcgac cgcgacagac gcgtgcactt ttccgcttca
     5101 gatgcaacaa
//
LOCUS       DASRZF010000364         3729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4067536, whole genome shotgun sequence.
ACCESSION   DASRZF010000364 DASRZF010000000
VERSION     DASRZF010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4067536"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..875)
                     /locus_tag="VFG22_16280"
     CDS             complement(87..875)
                     /locus_tag="VFG22_16280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417855.1"
                     /translation="MSLPVLVAIAGFFAVLGTSVFVAWLAFSVGRWLGSREALRADRE
                     HEGPVGSVVGATLGLLAFTLAITFNMAHTKYNDRKQLVREDVRAIETAYDRALLLPEE
                     ASHAAHEKLVEYARIRAFDTFANPDLPIAAIIARSEKIQQELWAQALAHRDNAQIRFY
                     MDALSAMRNIHAERVTVGIHDHVPIPVWIALIFVTALGMVTMGYQASLVGSKRARAMI
                     PLIVAFCVILTMALDLDRPQQGILRVDQGPMIRLDASLEHRSDR"
     gene            complement(958..1578)
                     /locus_tag="VFG22_16285"
     CDS             complement(958..1578)
                     /locus_tag="VFG22_16285"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6417856.1"
                     /translation="MKKTGPGVAMKRERMPQAERRRITRGRILDATIESLIEIGYPRT
                     TTVEVGERAGLSRGAQLHHFPSKADLLVAAIEHLADERSNEFEEALRARLDRGEEPID
                     TMVDMLWAMFSDPLYWATIELMVAARTDPELLEKLEAFERSLGGRIYRAFKELSGRGD
                     RDARIAVEMTLYFMRGLAMERIFRENDAHYDELVQRWKRQLHAMLG"
     gene            complement(1592..2770)
                     /locus_tag="VFG22_16290"
     CDS             complement(1592..2770)
                     /locus_tag="VFG22_16290"
                     /inference="COORDINATES: protein motif:HMM:NF006102.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HET6417857.1"
                     /translation="MGRKVNVIGVGMVKFAKPGQSEDYNVMAAKAIKAALADGGVDFG
                     EVEQAVAGYVFGDSTCGQRAVYDVGLTGIPVYNVNNNCSTGSSALMLAKQMVEGGIVE
                     CALAVGFEQMQKGALGSAWNDRPNPLGLHVNMMNETQGFTSAPPAAQMFGGAGREYRW
                     KYGAKRETFAKVNAKARQHAAKNPYALFRDLLTVEEILASPEVFDPLTRYQCCPPTCG
                     AAAAIVCSDDFAKKKGISNPVYIAGQTMRTDYGSSFGDSMIKMVGFDMAKNAAQAVYE
                     EAGIGPEDVDVVELHDCFTTNEVLTYEALGLCKEGEAEKFIEEGDNTYGGKFVTNPSG
                     GLLSKGHPLGATGLAQCTELVWHLRGQAEDRQVEGARVALQHNLGLGGAAVVTMYRRD
                     "
     gene            complement(2801..>3729)
                     /locus_tag="VFG22_16295"
     CDS             complement(2801..>3729)
                     /locus_tag="VFG22_16295"
                     /inference="COORDINATES: protein motif:HMM:NF026214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HET6417858.1"
                     /translation="SNVYPGDTLITEMWKESDPRIVFQCKVKERDGLVISNAAIELFE
                     KLPKPKEKKKTPTPAAEAPEAAAEPIAADVIAAIDHFLKENPAIAEKAQTVFQLKLSE
                     PESLWTIDLRAGNAGAGETAKPDVTLQLSEANYVALQRGEADPIKLFSTGKLKVGGDM
                     MSVNKLEALGAMPFELVLEKAAARASGGETPAPVAALKAREPLAPKLFEALATRLEEQ
                     PNLATEVGAVLQFYVRNPDSKWVVDLANQPPALKAGETDGATTTITIDDADLVELSSG
                     QMTTQALFQRGKLRVDGEVQPAHRLNFLKDLI"
BASE COUNT          630 a         1185 c         1144 g          770 t
ORIGIN      
        1 ccggttcgga tttccaggtg ccgaggccca gcgcgggcat cttcgttccg tcgagtagct
       61 ccaaggtctg catggttccc tccgaatcag cgatcgcttc gatgctcgag cgaggcgtcg
      121 agcctaatca tggggccttg atcgactcgt agaatgccct gttgagggcg gtccaagtcg
      181 agggccatgg tgaggatcac gcaaaacgcg acgatcagcg ggatcatcgc gcgagctctc
      241 ttcgaaccca ccagcgaggc ctgatagccc atggtcacca tcccgagcgc ggtcacgaag
      301 atgagcgcga tccagaccgg aatcggaacg tggtcgtgaa ttccgaccgt gacgcgctcg
      361 gcgtggatgt ttcgcatggc gctcagcgcg tccatgtaga atcggatctg cgcgttatcg
      421 cggtgagcga gcgcctgcgc ccagagctcc tgctggatct tctcggaccg agcgatgatc
      481 gccgcaatcg gcaggtcggg gttggcgaac gtgtcgaagg cccgaatccg agcgtactcg
      541 acgagtttct cgtgtgccgc gtgggaggcc tcttccggca gcagcaaggc gcgatcgtag
      601 gcggtctcga tcgctctcac gtcctcgcga accaactgct tgcggtcgtt gtacttggtg
      661 tgagccatgt tgaacgtgat cgccaaggtg aacgccagaa gcccgagcgt agcgccaacg
      721 accgagccga ccggaccctc gtgctcgcga tcggctcgca gcgcctcgcg cgaacccagc
      781 catcgtccga cggagaacgc cagccaggcc acgaaaaccg acgtacctag aaccgcgaag
      841 aatccggcaa tcgcgacgag taccggaagg ctcatgacaa acgatccatg attggactca
      901 ggcgtatatc gcagccggga ggcgcgctag caacttgcct ggaaggacgc tgggaattca
      961 gccgagcatc gcatgcagtt gccgcttcca gcgctgcacg agctcgtcgt agtgcgcgtc
     1021 gttctcacga aatattcgct ccatggcgag accgcgcatg aagtagagcg tcatctcgac
     1081 cgcgattcga gcgtcacgat cgccacggcc gctgagctcc ttgaacgctc gatagatccg
     1141 gccgccgagg ctgcgctcga atgcctcgag cttctcgaga agctctggat cggttcgcgc
     1201 cgccaccatg agctcgatgg tcgcccaata gagcgggtcg gagaacattg cccagagcat
     1261 gtccaccatc gtgtcgatcg gctcttcgcc ccggtcgagg cgagccctca gcgcttcttc
     1321 gaattcgttc gagcgctcgt cggcgagatg ctcaatcgcc gcgaccagca aatccgcttt
     1381 gctcggaaag tgatggagct gagctcctcg ggagagtccg gcgcgctcgc ccacttcgac
     1441 ggtcgtggtc cggggatatc cgatctcgat caggctctcg atggtcgcat cgaggattct
     1501 gccacgggtg atgcggcgcc gctccgcttg cggcatgcgt tctcgcttca tagcgacgcc
     1561 gggtcccgtc ttcttcacgc cgatctgctc ctcagtcgcg ccggtacatc gtgaccacgg
     1621 ctgcaccgcc gaggccgagg ttgtgctgca gcgcgacccg agcaccctcg acctggcgat
     1681 cctctgcctg accgcgaaga tgccaaacca gctcggtgca ttgcgccagt ccggtcgcgc
     1741 cgagcggatg ccctttcgaa agaaggcccc cactcggatt ggttacgaat ttgcccccgt
     1801 aggtgttgtc accctcttcg atgaacttct cggcttcgcc ttccttgcaa agccccaggg
     1861 cctcgtaggt gagcacttcg ttggtggtga agcagtcgtg gagctcgacg acgtcgacat
     1921 cttcgggccc gattccggcc tcctcgtaca ctgcctgcgc ggcgttcttg gccatgtcga
     1981 agccgaccat cttgatcatc gagtcgccga agctcgagcc gtagtcggtc cgcatcgttt
     2041 ggcctgcgat gtagaccgga ttcgaaatgc ccttcttctt tgcgaagtcg tccgagcaga
     2101 cgatcgccgc cgctgcgccg caggtcgggg gacagcactg gtagcgcgtg agcggatcga
     2161 aaacctcggg cgaggcgagg atctcttcga cggtgagcag atcccggaag agcgcgtagg
     2221 ggttcttcgc agcgtgctgg cgcgcctttg cgttcacctt ggcgaacgtc tctcgcttgg
     2281 caccgtactt ccaacgatac tcacgacccg ctccaccgaa catctgcgcg gcggggggcg
     2341 ccgaggtgaa accctgggtt tcgttcatca tgttcacgtg aaggccgagg ggattcggcc
     2401 gatcgttcca ggcgcttccg agcgcgccct tctgcatctg ctcgaagccc acggcgagcg
     2461 cgcactctac gatgccgccc tcgaccatct gcttggccag catcagggca ctcgaaccgg
     2521 tcgagcaatt gttgttgacg ttgtagaccg gaattcccgt gaggccgaca tcgtagaccg
     2581 cacgctgtcc gcaagtgctg tctccaaaca catagcctgc gacggcttgc tcgacctcgc
     2641 cgaagtcgac gccgccatcg gccaaggccg ccttgatcgc cttggcggcc atcacgttgt
     2701 agtcctcgct ctggccgggc ttggcgaact tcaccatgcc aactccgatt acgtttacct
     2761 tgcgtcccat cgttgctcct tcgcgctcct tcgcgcgaga ttaaatcagg tccttgagaa
     2821 agttgagtcg gtgtgcaggt tggacttcgc catccacgcg aagtttgcct cgctgaaaga
     2881 gggcctgcgt tgtcatctga ccgctgctga gctctacgag atccgcgtca tcgatcgtga
     2941 tggtcgtagt ggcgccgtcg gtttcgcccg ccttgagcgc cggcggctgg ttcgcgagat
     3001 cgacgaccca cttggagtcg gggttccgaa cgtagaactg aagcaccgcg cctacctcgg
     3061 tcgctagatt gggttgctcc tcgagtcgcg tcgcgagcgc ttcgaacagc ttgggcgcaa
     3121 gcggctcgcg tgccttgagt gcagcgaccg gcgccggcgt ttcaccaccg cttgcgcggg
     3181 cggcggcctt ctcgaggacg agctcgaagg gcatggctcc gagcgcttcg agcttgttga
     3241 cgctcatcat gtcgccgccc accttgagct tgccggtcga gaagagcttg atcggatcgg
     3301 cttcacctct ttggagcgcg acgtaattgg cttcggaaag ctgcagggtc acgtcgggct
     3361 tcgccgtctc ccccgccccg gcattccccg ccctgaggtc gatcgtccag agggactcgg
     3421 gctcgctcag cttgagctgg aacaccgtct gtgccttctc cgcgatcgct gggttttctt
     3481 tcaagaagtg atcgatggcg gcaatgacgt cggcggcgat cggctctgcg gcagcctcag
     3541 gtgcttcggc tgctggcgtc ggcgtcttct tcttctcttt gggcttcggc agcttttcga
     3601 agagctcgat ggccgcgttg gaaatcacca gcccgtctcg ttccttgacc ttgcattgaa
     3661 acacgatgcg cgggtcggat tccttccaca tctccgttat gagcgtgtcg ccagggtaca
     3721 cgttgctgg
//
LOCUS       DASRZF010000365        10019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1655801, whole genome shotgun sequence.
ACCESSION   DASRZF010000365 DASRZF010000000
VERSION     DASRZF010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 10019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10019
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1655801"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="VFG22_16300"
     CDS             complement(<1..739)
                     /locus_tag="VFG22_16300"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HET6417859.1"
                     /translation="MWADRDNIATFVEVVRRNSLAAAARYLEVPKSTVSRRLSRLEEQ
                     LDTKLVHRDARRVTPTPEGIRFYESVVDAVDVLDTAVAMIERSSTEPRGTIRLTAPSD
                     LGRMLLIAELSRFLDRYPDISLDLMFTNRFVDLVQERVDVALRAGEVTEPNLIARKLL
                     PSDFQLAAHPQHEIDCHDISGLEAHPFVLYRRRGRTQVFRLEKGSGEEQETVELTVSG
                     RVSVDDYSAMVELVAAGQGLGLMPELHV"
     gene            824..1837
                     /locus_tag="VFG22_16305"
     CDS             824..1837
                     /locus_tag="VFG22_16305"
                     /inference="COORDINATES: protein motif:HMM:NF017536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HET6417860.1"
                     /translation="MVIEQSRRGVLATIGAAIAALAAWGISRKLLGRAESEASMKAQS
                     KNTPAGARPLQGVYPPGPEHWVGDGFFVKTVFWPQLGAEMLSPFLLLDHAKRKHFDPS
                     PWRRGVGEHPHRGFETVTFAYEGEVEHRDSAGGGGRIGPGDVQWMTAASGVVHEEKHS
                     QAFSERGGEFEMVQLWVNLPASQKMSAPRYQPLLDREFPRIQLGAAEARLVAGELGSA
                     RGPARTHTPIATFDLRFGEDGTSEVELPAGWTTLVFSLEGDLSAGPEHTPIPARHFGV
                     FERNAAGAVQVHATKGTRALVLAGEPLGEPVVAYGPFVMNTPEEIGQAFRDYQSGKMG
                     RLL"
     gene            1857..2858
                     /locus_tag="VFG22_16310"
     CDS             1857..2858
                     /locus_tag="VFG22_16310"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF024801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET6417861.1"
                     /translation="MGKLVDGAWRDIWYDTKSTGGKFRRSNSEFRNWITPNGKNPPNG
                     KAFEAEAGRYHLYVSYACPWAHRTLIFRKLKGLEDMIGVSVVNHFMGGQGWTFHEGDG
                     VIPDPILNAEFLHEIYTAAELRFTGRVTVPVLWDKKTETIVSNESSEIIRMFNSAFDH
                     LGAKPGDFYPETLRAEIDEVNERVYATLNDGVYKCGFATTQEAYEEAFVALFDTLDWL
                     EERLGQQRYVAGDQITEADWRLFTTLVRFDAVYFSHFKANLRRIVDYPNLWAYTRELY
                     QVPGVKGTVNMHHIKHHYYGSHQTINPTGIVPMGPVIDFEAPHRRDSMLDPLSDAAE"
     gene            complement(2874..3452)
                     /locus_tag="VFG22_16315"
     CDS             complement(2874..3452)
                     /locus_tag="VFG22_16315"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET6417862.1"
                     /translation="MVRFASLGFSALALVLATSPVHAAGEPEVRTAQIVEEGSKISWG
                     HAIAVIDSPIDEVLPVVVDYANYVLFMPNFTTSKVLTQRGNRAMVYMEVSVASGTFTL
                     WGQLNFSESAPEGETRVIEAQLLEGNMRAFRASWRLTPVEGGARTEVDFKIYVDPDMP
                     LPSSVFSRENEKAAGKTLRALNTRVRDSGKRS"
     gene            complement(3667..4647)
                     /locus_tag="VFG22_16320"
     CDS             complement(3667..4647)
                     /locus_tag="VFG22_16320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417863.1"
                     /translation="MNWSSPQIVAWTLVLSVFAFGCDSSSSTDKSDPCLTALRINLEV
                     ADGTVIDEVEYVISGNGMEPMGGTIDTSAPGATASVESFGIPPGEGYIVTMVASSVDG
                     SLMCGGSASFDVGPGVSTEVEVMLHCKGSQQFGGVRVNGKLNICAELEKVIVAPLQTA
                     SGYALEVSANASDEEGDPVDYSWTATGGSFGDASAAATVFTCGEASEEQITIEVSDDA
                     FEYCADSWTIDVNCVDDVGTGGTGGDGGGGGTAGSGGTGGSAGSGGTAGSGGSGGTAG
                     SGGTGGSAGSGGTGGSAGSGGTGGSAGSGGGTAGNGGGAPPECTVSVSVD"
     gene            4923..5957
                     /locus_tag="VFG22_16325"
     CDS             4923..5957
                     /locus_tag="VFG22_16325"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET6417864.1"
                     /translation="MELEPLDGKRCLVTGGAGYLGRNLVAALRARGCRVHVLDRAPSP
                     GKQEGVYWFAGDVRSYPDMQAACEGVDTIFHTAAVIEAITHARRSVVERIESINVGGT
                     ENVIRVAQERGVRRLVHTSSIVASFCMDAAGSDESAPYSNSRDLYTATKIAGERAALG
                     ANGQKGLLSCAIRPGGIYGPGERLTYGRLVRALKMGVPLVVFGDGRARLDYVYIDSLV
                     DAELRAAERLVEGSPVCGQAYFVSDETPINAGQFSIELVKNMGLEPRLVRVPDPVARG
                     MGTAFERIYQLFGVVKPLFTLANVKLCDIDHYFSIDKAKQHLGYVPLVDTEEGLRRTA
                     VEARAYYDSL"
     gene            6001..7140
                     /locus_tag="VFG22_16330"
     CDS             6001..7140
                     /locus_tag="VFG22_16330"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6417865.1"
                     /translation="MILTEQQEMVRESAARFAEERLAPNSRAWEAAGEVELSALREMG
                     ELGFMGMTVPEAYHGAGLDYVSYALALIEIAAGDGAVSTIMSVNNAPVCAILQSAASE
                     TLRERYLKPLAAGEMIGAFALTEPQAGSDAANQQTRATRTPDGYSITGTKQFITSGRI
                     AGAVIVFAVTDPSAGKRGISAFLVPAGTPGMSVASLEHKMGQEASDTAQLSFEDAHVP
                     TDHLIGNEGDGYRIALANLEAGRIGIAAQCVGMARAALDHALAYAKERTAFGKSISEH
                     QAVAFRLADMATQVHAATTMVLEAAELRDAGRPCLEQACMAKLFASEAAERVCSDAIQ
                     IFGGYGYSRELPVEKIYRDVRVAQIYEGTSDVQRIIISRELLRGA"
     gene            7183..7683
                     /locus_tag="VFG22_16335"
     CDS             7183..7683
                     /locus_tag="VFG22_16335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417866.1"
                     /translation="MFRHFAIRDLTILVATIVLWWMSVTGDSGTELTSAFSIAAGVGA
                     AISAYNLHEWGHLLGAHWTQSVYVPAKRWYSPFLFSYDAERNTRGQFLLMSLAGFAAT
                     ALFVIGFLLWMPRDQVAGRIALRGALILAGLTAVIELPIFFRALFGNSVPRTGMFKGP
                     TVGGRD"
     gene            7771..9750
                     /locus_tag="VFG22_16340"
     CDS             7771..9750
                     /locus_tag="VFG22_16340"
                     /inference="COORDINATES: protein motif:HMM:NF005539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6417867.1"
                     /translation="MSSANEAFVTGGTGLIGRALVERLLRRGVNVTLMLRDDAGERRK
                     TELDVLRVFAKGAEGSLSEVAGDLSRPELGLTQAGAAALARAGHCFNLAALYDIEADP
                     ESIEKTNVEGTKHLLSALRSAGFQGRLHHMSSIAVAGDYAGTFTEAMLEEGQGFPHAY
                     HRSKLESEKLVRESGFDYRVFRPSGVVGDSKTGAMDRIDGVYFGFGAIKKIAYALPGW
                     VRLPVPKVRGGFNLVPVDYVADALAQIAFAKTDARVFHLVDPKPPSLTKMTDMFVAAA
                     GGPRLGPAIDFAKMPGMKKTGALVSMLPSVRELRDALLSDLGLPAGGLGMMNIKARFD
                     ESNTRAALEGSGIHCPPLHSYAKTLYRYYEDHLDPMKQRPARYRRVLTGKVVLVTGAS
                     RGIGLVVAEMAAEAGAKVLLVARDASKLEEAVARIRAAGGDAHAHVADLSSFDEVDAL
                     AEAVLAQHGGVDVLIHNAARSIRRPIVASIDRFHDYERTMALNYFAPVRLTLRFLPSL
                     RERGGTISHVLTMGVLIPGPYFAAYLATKSALDAFGDSLMSELQHEGIHVSSVYLPLV
                     KTEMMAPVEEYAGRRDVMTPKRAATMILDGVVDRQRRVMTPVGRFFSFSNRITPATTS
                     RILNLLERTFPIGDTPSEFPMEKAFITNAIGGSPI"
     gene            complement(9747..>10019)
                     /locus_tag="VFG22_16345"
     CDS             complement(9747..>10019)
                     /locus_tag="VFG22_16345"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET6417868.1"
                     /translation="PAFEALSQELAEEPIRFVRINTAEQPALSQPFHIHSVPTLLFVN
                     DGEILDVRVGALPGPQLAKKAQWLLRKARGEGFFSGLFGGKQTRES"
BASE COUNT         1866 a         3188 c         3159 g         1806 t
ORIGIN      
        1 cgacatgaag ctcgggcatg agcccaaggc cctgccctgc ggcaacgagc tcgaccatcg
       61 cgctgtagtc gtcgacgctc acgcggcccg agaccgtgag ctcgacggtc tcctgctctt
      121 cgccgcttcc cttctcgagt cgaaacacct gggtccgtcc gcgcctgcga tagagaacga
      181 aggggtgcgc ttcgaggccg ctgatgtcgt ggcaatcgat ctcgtgttgc ggatgagccg
      241 cgagctgaaa atccgacgga agcagtttgc gcgcgatgag attgggttcg gtgacttcgc
      301 ctgcacgcaa agcgacgtcg acacgctctt ggacgagatc gacaaatcga ttggtgaaca
      361 tgagatcgag cgaaatgtca gggtaccgat cgagaaaccg agaaagctcc gcgatgagga
      421 gcatgcggcc gagatcgctg ggcgctgtca accgaatcgt tccccgaggc tcggtgctgc
      481 ttcgctcgat catcgcgacc gccgtgtcga gcacgtcgac tgcatccacc actgattcgt
      541 agaaacggat gccctccggc gtgggcgtca cgcggcgcgc gtcccggtgc acgagcttgg
      601 tgtcgagctg ctcctccaga cgcgaaagcc gccggctcac ggtcgacttg gggacctcga
      661 gatatcgcgc tgcggccgcc aggctattgc gccgtaccac ctccacaaac gttgcaatat
      721 tgtccctgtc agcccacatg gcgcttataa gttgcgttaa cgcaactcta cgtcaagtcg
      781 atgcgctctg tcgagggtgg gcggcactcc ttacactccg ccggtggtga tcgagcaatc
      841 gaggcgaggt gtgctggcga cgatcggtgc cgcaatcgcg gcactcgccg cttggggcat
      901 ttcgcgcaag ctgctcggac gcgccgagtc ggaggccagc atgaaagccc aatcgaagaa
      961 cacgccagca ggcgcgcgac cccttcaagg cgtgtacccg ccaggccctg agcactgggt
     1021 cggcgacggc ttcttcgtga agaccgtgtt ctggccgcaa ctcggtgccg agatgctgag
     1081 cccgtttctt ctgctcgatc acgcgaaacg gaagcatttc gatccgtctc cgtggcggcg
     1141 tggggtcgga gagcatccgc accgcggctt cgagaccgtg accttcgctt acgagggcga
     1201 ggtggaacac cgggactccg cgggcggggg cggtcgcatc ggacccggcg atgtgcagtg
     1261 gatgaccgcg gcctccgggg tcgtgcacga ggaaaaacac tcccaggcgt tctcagagcg
     1321 cggtggcgag ttcgagatgg tgcagctctg ggtgaacctg cctgccagcc agaagatgag
     1381 cgcgccgcgc taccagccgc tcctcgaccg cgagttcccg cgcatacaac tcggcgccgc
     1441 cgaagcacga ctggtggccg gtgagctcgg ctccgcgaga ggcccggccc gaacgcacac
     1501 gccgatcgcg accttcgacc ttcggttcgg tgaagatggt acgtccgagg tcgagcttcc
     1561 cgcgggctgg acgaccctcg tgttttctct cgaaggggac ctcagtgcag gaccggagca
     1621 cacgcccatc cctgcacggc acttcggtgt attcgaacga aacgcagcgg gagcggtgca
     1681 agttcatgcc acaaagggga cgcgtgccct cgtgctcgcg ggcgagccgc tcggtgagcc
     1741 ggtagtggcc tacggcccct tcgtcatgaa cacaccagaa gaaattggac aggccttccg
     1801 cgactatcag agcgggaaga tgggccgtct gctttgactc ctaggaaggt acgaaaatgg
     1861 gaaagctagt tgacggcgct tggcgcgaca tttggtacga cacgaagagc acgggcggga
     1921 agttccggcg ttcgaacagc gagttccgga actggatcac cccaaacggc aagaacccgc
     1981 cgaatggaaa ggccttcgaa gcggaagcgg gtcgctacca cctttacgtg tcctatgcct
     2041 gcccatgggc gcaccggacc ttgatctttc gcaagctcaa gggtctcgag gacatgatcg
     2101 gggtctccgt cgtgaatcac ttcatgggcg gtcagggctg gacgtttcat gagggcgacg
     2161 gcgtcattcc cgatccgatc ctgaacgccg agttcctaca cgagatctat acggcggccg
     2221 agctgcggtt taccgggcgc gtgaccgtgc ccgtgctctg ggataagaag accgagacca
     2281 tcgtgagcaa cgagtcgtcc gagatcatcc gcatgttcaa cagcgcgttc gatcatttgg
     2341 gcgccaagcc cggcgacttt tacccggaga cgctccgggc cgagatcgac gaggtgaacg
     2401 agcgcgtgta cgcaacgctc aacgacggcg tgtacaaatg cggattcgct accactcagg
     2461 aagcctacga ggaagcgttc gtcgccttgt tcgatacctt ggactggctc gaagagcgcc
     2521 tcggacagca gcgctacgtc gccggcgatc agatcacgga agccgactgg cgcttgttca
     2581 ccacgttagt tcgattcgat gcggtctatt tcagtcattt caaagccaac ttgcgacgca
     2641 tcgtcgacta tccgaatctc tgggcgtaca cgcgcgagct ctatcaagtc cctggcgtca
     2701 agggcactgt caacatgcat cacatcaagc accactatta cggcagccac cagaccatca
     2761 atcccacggg catcgtcccc atggggccgg tgatcgattt cgaagcgccg caccgacgcg
     2821 acagcatgct ggatccgctg agcgacgccg ctgagtaacg aacacaaaga gagtcaggag
     2881 cgcttgcctg agtcccggac gcgcgtgttg agggcgcgca aggtcttgcc ggccgccttt
     2941 tcgttctcac ggctgaacac cgatgagggc aagggcatgt cgggatcgac gtagatcttg
     3001 aagtcgacct cggttcgggc gccaccctcg accggagtca ggcgccagct cgctctgaac
     3061 gcgcgcatgt tgccctcgag cagctgggcc tcgatcacgc gcgtctcgcc ctctggcgcg
     3121 ctttcggaga aattcaactg gccccaaagc gtgaaggtcc cggacgccac gctgacctcc
     3181 atgtagacca tcgcccgatt gccgcgctgc gtgagcacct tggacgtggt gaagttgggc
     3241 atgaagagaa cgtagttggc gtagtcgacg acgaccggga ggacctcgtc gatcgggctg
     3301 tcgatcactg cgatcgcatg accccaggag atcttcgagc cttcctcgac gatctgcgcg
     3361 gtccgaacct ccggctcacc ggccgcgtgg accggactcg tggccaagac gagcgccaat
     3421 gcgctgaagc caagagaagc aaatctaacc atccgggaat tctgcctagg gtcgctacaa
     3481 caatccattt cctgcctccg gtgagcggca gcattgcaga ggccgtgcca atgctccgat
     3541 gggcaaaaac tcccagggta tccaaggctt gcggcgattc cgctcacaac gtagcagagc
     3601 tcggtcggtc aagaatgtcc gactggcaac tggagtagct acctcgaggc tgcgctcgga
     3661 agacctttag tcgaccgaaa cggagacggt gcactcaggc ggggctccgc cgccattgcc
     3721 agccgtgccg ccgccgctgc ccgcgcttcc gcccgtgccg ccgctgcccg cgctgccgcc
     3781 cgtgccgccg ctgcctgcgc tgccgcccgt gccgccgctg cctgcggttc caccgctgcc
     3841 gccgctgcct gcggttccac cgctgcctgc gctgccgccc gtgccgccac tgcctgcggt
     3901 tccaccgccg ccgccgtcac caccggtacc accggtacca acatcgtcga cgcaattcac
     3961 gtcgatggtc cagctatccg cgcaatactc aaacgcgtcg tcggacacct cgatggtgat
     4021 ctgctcttcg ctcgcctctc cgcaggtaaa gaccgtggct gccgcgcttg cgtcgccgaa
     4081 ggaaccaccg gtcgcggtcc agctgtagtc gacgggatcg ccttcttcgt cacttgcatt
     4141 tgcagatact tcgagtgcgt atccacttgc ggtctgaagt ggcgcaacga tgaccttctc
     4201 gagctccgcg cagatgttga gcttgccgtt cacccgaacg ccaccgaact gctgagatcc
     4261 cttgcaatgg agcatgacct cgacctcggt gctcacaccg gggcccacat cgaagcttgc
     4321 cgaaccgccg cacatcaagc ttccatcgac cgagctcgcg accatcgtga cgatgtagcc
     4381 ctcaccggga ggaataccga agctctcgac cgaagcggtg gcgccgggcg cgctcgtgtc
     4441 gatggtgccg cccatgggct ccatcccgtt gccgctgatc acgtactcga cctcatcgat
     4501 aaccgtgccg tccgccactt cgagattgat gcgaagagcg gtgaggcagg gatcgctctt
     4561 gtcggtgctg ctgctgctgt cacagccgaa ggcgaaaacc gagagcacga gtgtccaagc
     4621 aacgatctgt ggcgagctcc aattcatggt cgtcttcttc ctcggtacta gctccggcgg
     4681 gaggggggaa accgccggat acactgcggg cgcagaggag ccagtctagc ctcaagtcgg
     4741 gtgcgcgagt caatccccaa cttacgtatc gttccaaaat tgttgggaga aatgcatttg
     4801 ggcgcgccta tttgtgagct tcgttacaag ccgcacgtgc acatcgaccc agcggctcga
     4861 tgagggtggc agagccaacg ctgcgcgagc cgcaccccag tgctatcagg tgcgccgcgc
     4921 agatggagct cgaaccactc gatgggaagc gatgcctcgt gaccggaggc gctgggtatc
     4981 ttgggcgaaa tctcgtggcg gcgttgcgag ctcggggttg tcgggtccac gtgctcgata
     5041 gagctccctc gcctggaaag caggaaggag tttactggtt tgccggcgac gtccgaagct
     5101 acccggacat gcaagcggca tgcgagggcg tggatacgat attccacacg gcggccgtca
     5161 tcgaagcgat cacgcacgcg cgccgctcgg tagtggagag gatcgagtcg atcaacgtcg
     5221 gcggaactga gaacgtcatt cgagtcgcgc aggagagggg cgtgcggcga ctggtgcata
     5281 ccagctccat cgtggcgtcg ttctgcatgg atgcagcggg cagcgacgag tccgccccgt
     5341 acagcaattc gcgcgacctc tataccgcca cgaagatcgc cggcgaacgc gcggcactcg
     5401 gcgccaacgg gcagaaggga ctgctgagct gtgcaattcg tcccggaggg atctacgggc
     5461 cgggcgagcg gctgacctac gggcgcctgg tccgtgcgct caagatgggc gtgcctctgg
     5521 tcgttttcgg cgacggtcgc gcgcggctcg actacgtcta catcgacagc ctcgtcgacg
     5581 cggagctccg tgccgccgag cgcctggtcg aagggtcgcc ggtctgcggc caggcgtact
     5641 tcgtgagcga cgagaccccg atcaacgccg gtcagttcag catcgagctc gtgaagaaca
     5701 tgggcctcga accccgcctg gttcgcgtgc ccgacccggt cgccaggggc atgggcaccg
     5761 ccttcgagcg catctatcaa ctctttggcg tggtcaagcc cttgtttact ttggcgaacg
     5821 tgaagctctg cgacatcgac cattatttct cgatcgacaa ggcgaagcag catctcgggt
     5881 atgtgccact ggtcgatacc gaagaaggcc tgcgccgcac cgccgtcgag gcgcgtgcct
     5941 actacgacag cctttgaccg actagcgccg cgagaccggc tgttctattc cgaacgggca
     6001 atgattctca ccgagcaaca ggaaatggtt cgcgagtcgg cggcgcgatt cgcggaagag
     6061 cggttggccc ccaacagccg cgcctgggaa gcagcaggcg aggtcgagct cagcgccttg
     6121 cgcgagatgg gcgagctcgg gttcatgggg atgaccgtac cggaggcgta ccacggagct
     6181 gggctcgact atgtctccta tgcgctcgcg ttgatcgaga ttgcggccgg cgatggcgcc
     6241 gtctcgacca tcatgagcgt caacaacgca cccgtatgcg cgatcctaca atccgcagcg
     6301 agcgagacac tgcgcgaacg gtacctcaag cccctggctg caggagagat gataggcgcg
     6361 ttcgcgttga cggagccgca agccgggtcc gatgccgcga accaacaaac gcgcgccacg
     6421 cgaacgccgg acggctactc gatcacgggg accaagcagt tcatcacctc gggcaggatt
     6481 gctggcgccg tcatcgtctt tgcggtgacg gacccttcag ccgggaagcg cggaatttcg
     6541 gcgtttctcg ttccggccgg cactccgggg atgagcgtcg cctcactcga gcacaagatg
     6601 ggacaagaag cgtcggacac cgcccagctc agcttcgaag atgcccacgt gcccaccgac
     6661 catctcatcg gaaacgaagg agatggatat cgaatcgcgc tggccaactt ggaggccggg
     6721 cgcatcggta ttgccgctca gtgcgtgggt atggctcgtg cggcgctcga ccatgctctg
     6781 gcctatgcca aagagcgaac cgctttcgga aagtcgatct ccgagcatca ggcggtcgca
     6841 ttcaggctcg cagacatggc gacccaggtt catgccgcaa ccacgatggt gctcgaagcc
     6901 gcggagctcc gtgacgccgg gcgtccctgc ctcgagcagg cctgcatggc gaaactgttt
     6961 gcgtccgagg ccgccgagcg cgtttgctcc gatgccattc agattttcgg aggatatggg
     7021 tactctcgcg agcttccggt cgagaagatc taccgcgacg tgcgggttgc acagatttac
     7081 gagggaacgt cggacgtgca gcgcatcatc atcagccggg agctgcttcg cggcgcctga
     7141 gccctccctt gacgcccggc gccgggggcg tagaaagcgg aggtgtttcg ccacttcgcc
     7201 attcgagatc tgacgatcct cgtcgcgaca atcgtgcttt ggtggatgag cgtcacgggc
     7261 gattcgggaa ctgagctcac ctcggcgttt tcgatcgccg ctggtgtagg cgctgccatc
     7321 tcggcgtaca acctccacga gtggggccat ttgcttggcg cgcactggac ccagagcgtc
     7381 tacgtgcccg cgaaacgatg gtattcgccg ttcctcttca gctacgatgc ggagcgcaac
     7441 acgcgcggcc aatttttgct gatgtccctc gccggcttcg ccgcgaccgc gctcttcgtg
     7501 atcgggttcc ttctctggat gccccgcgat caagtggccg gacgcatcgc gcttcgcgga
     7561 gccctcatcc tagcgggtct caccgcggtc atcgagctgc cgatcttttt ccgggcgctc
     7621 ttcggcaaca gtgtgccgcg taccggcatg ttcaagggtc caacggtggg cgggcgcgac
     7681 tgaggcctgc aagtcggcaa agccgctcgg cgccgacatg catgcagggc cgctggcaca
     7741 atcgatgcgc tcggttcatt ctcgcttgtg atgagctcag cgaatgaggc gtttgtaacg
     7801 ggtggaactg gactgattgg acgcgcgctg gtcgagcgcc tactgcgccg cggtgtgaat
     7861 gtcaccttga tgcttcggga cgacgcgggt gagcggcgca aaacggagct cgatgtgctc
     7921 cgggtgtttg ccaagggcgc tgagggctcg ctcagcgagg tcgcaggcga tctttctcgg
     7981 ccggagctcg gcctaaccca ggcgggcgcc gctgcactcg ctcgtgcggg tcactgcttc
     8041 aacctggccg ccctctacga catcgaggcc gaccccgaat cgatcgagaa gacgaacgtc
     8101 gaaggcacga agcacttgct ctcggccctc cgaagcgccg gctttcaggg gcggcttcat
     8161 catatgagct caatcgcagt cgcgggcgac tacgcaggga cattcaccga ggcgatgctc
     8221 gaagagggcc aagggtttcc tcacgcgtat catcggagca agctcgagtc ggagaagctc
     8281 gttcgggagt ctggattcga ctatcgcgtc tttcgcccga gcggggtggt gggagactcg
     8341 aagaccgggg ccatggaccg catcgacggc gtctacttcg gctttggagc gatcaaaaag
     8401 atcgcctacg cgctccccgg ctgggtccgc ctccccgtgc ccaaggtccg cggcggcttc
     8461 aacctggttc cggtcgacta cgtcgcggac gcgctggctc agatcgcgtt cgccaagacc
     8521 gacgcgcgcg tgttccacct cgtcgacccg aagcctcctt ccctcacgaa gatgaccgac
     8581 atgttcgtgg ccgccgcggg tggaccacga ctgggcccag cgatcgactt cgccaagatg
     8641 cccggcatga agaagaccgg cgcgctcgtc tcgatgctcc cgagtgtccg tgagctccgc
     8701 gatgcgctcc tgagcgacct cggacttccc gccggcggac tcgggatgat gaacatcaag
     8761 gcccgcttcg acgagagcaa cacgcgggcg gcgctagagg gctcaggaat tcactgccct
     8821 cccctccaca gctacgccaa aacgctctac cgctactacg aagaccatct cgacccgatg
     8881 aagcagcggc cggcacgata ccggcgggtc ctcactggca aggtagtgct cgtcaccggt
     8941 gcatcgcgcg gaataggcct ggtggtcgcc gagatggccg ccgaggcggg ggccaaagtc
     9001 ctgctggtcg cgcgggacgc atcgaaacta gaggaggccg ttgcacgaat tcgcgcggcc
     9061 ggcggcgatg cgcatgcaca tgtggcggac ctctcgtcct tcgacgaggt cgatgcgctg
     9121 gcggaagccg tgctcgcgca gcacggcggt gtcgatgtgc tcatccacaa tgcggctcgg
     9181 tcgattcggc gcccgatcgt cgcctcgatc gatcggttcc acgactacga gcgcacgatg
     9241 gccttgaact actttgcacc cgtccggctg accctgcgct tcctaccctc gcttcgggag
     9301 cggggcggaa cgatctcgca cgtcctgacc atgggagtgc tcattccagg accgtacttc
     9361 gctgcatacc tggccacgaa gtccgctctc gacgccttcg gcgactcgct gatgtccgag
     9421 ctgcaacacg aaggcatcca cgtctcctcc gtctacttgc cgctcgtgaa gaccgagatg
     9481 atggcgcctg tcgaggagta cgccggacgc cgcgacgtca tgaccccgaa aagagccgca
     9541 acgatgatcc tcgacggggt ggtcgatcgc caacgccgcg tgatgacccc ggtgggccgg
     9601 ttcttctcat tcagcaaccg aatcaccccg gcgaccacca gccgaatctt gaatctcctc
     9661 gagcgaacgt tcccgatcgg agacacgccg agcgagttcc cgatggagaa agcgttcatc
     9721 acgaacgcga tcggcggaag cccaatctag ctctcacgcg tttgctttcc cccgaaaagg
     9781 ccggaaaaga aaccctcgcc gcgcgcctta cgaaggagcc attgcgcctt tttggcaagc
     9841 tgagggccag ggagcgcccc aacgcgaacg tcgaggatct ccccgtcgtt gacgaagagc
     9901 aaagtcggta ccgaatgaat gtggaatggc tgagagagcg ccggctgctc ggcggtgttg
     9961 atccgcacga agcggatcgg ctcctcggcg agctcctgcg aaagcgcctc gaaggcggg
//
LOCUS       DASRZF010000366         2438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_276358, whole genome shotgun sequence.
ACCESSION   DASRZF010000366 DASRZF010000000
VERSION     DASRZF010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276358"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="VFG22_16350"
     CDS             <1..478
                     /locus_tag="VFG22_16350"
                     /inference="COORDINATES: protein motif:HMM:NF016994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HET6417869.1"
                     /translation="HNSHVGDARATEMGASRGEHNIGQLCRQAFGDAAFSIGFGTHTG
                     TVAAATDWGGPMQIKKVRPSHPSSYERLCHDSGVPAFLLALRYPTRESVREELASPRL
                     ERAIGVIYRPETELQSHYFQAALPWQFDAYAWFDETRAVHALDADAAHAVIPHVYG"
     gene            506..649
                     /locus_tag="VFG22_16355"
     CDS             506..649
                     /locus_tag="VFG22_16355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417870.1"
                     /translation="MSKQNEQEGPARHSELTSLRKRIEALEARVTELEKELREAQELA
                     PTD"
     gene            731..>2438
                     /locus_tag="VFG22_16360"
     CDS             731..>2438
                     /locus_tag="VFG22_16360"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HET6417871.1"
                     /translation="MSPYFQAIAVDFDGTITELGRPSARTLAALSEVRAAGVKTLLVT
                     GRVVTDLMHVFPDAEQWFDVIVAENGAVIRRDGVSHALTSPVPLELDEPLVAQGVYFQ
                     RGQVLLACSGDDELTVLREVRRLEADCQLVRNRAALMVLPSEVSKGWGLAEALKGLGV
                     SYHSTIGIGDAENDLSLFRHCELGVAVGNAVESLKREADIVLSGSDGVAVADFLQNQL
                     LVEESLPPSRRWRVTIGLLDDGSGVSLPASRVNILFVGESGVGKSYAAGLLAERLIEL
                     DYSVCFIDPEGDHAPLGRLPKVLTVGGRGLLPDARDVPKLMSQQLGSVIVDLSFLAEA
                     ERTPYTAELLRALREERSNSCLPHWIMIDEAHMPFGAGGEVCGMLGGQKGLCLVTYDA
                     TRLCRSSGLAFDFLVAIPGQEGLDPAAAESIRELSGIDPFGEFSDPKQGEAWLVRLDG
                     PPRAERFRLGRRFVRHVRHWHKYAEARLPPARVFRFRSFSGPSGAQADNLSAFRRELL
                     LCDSKVLQHHAANHDFSRWLEHAISDETLARVAHELEKRSAVDASFEDLRRGLVEAIE
                     DRY"
BASE COUNT          452 a          733 c          776 g          477 t
ORIGIN      
        1 gcacaattcg cacgtgggcg acgcccgcgc gacggagatg ggcgcatcgc gaggcgagca
       61 caacatcggg caactgtgcc gccaggcctt tggggatgcc gcattttcaa ttggctttgg
      121 cacccataca ggaacagtgg cggccgccac ggactggggt ggaccgatgc agatcaagaa
      181 agtgcgcccc tcgcacccga gtagctacga acgtctttgc cacgactccg gcgttccggc
      241 gtttctgctg gcgctccgct atccgacacg cgagtcggtg cgcgaagagc tcgcttcgcc
      301 gcgcctggag cgggctatcg gcgtgatcta ccgtccggaa accgagcttc aaagccacta
      361 cttccaagcg gccctgccct ggcagttcga cgcttacgcc tggttcgacg aaacccgcgc
      421 cgtccacgcg ctcgatgcgg atgcggcaca cgcggtgatt ccacatgtct acggatgaag
      481 cacctcaatc aagaaggcga atatcatgtc aaaacagaac gaacaggaag gccctgcgcg
      541 acacagcgag cttacctcgc taaggaagcg catcgaagcc ctcgaagcac gcgtcacgga
      601 gctcgagaaa gagctgcgtg aggctcagga gctcgcgccc actgactagt gtggttcgtg
      661 cgccttacgg aacgctgatc gtggagcgtc cgatgcaaat gagcttggcg cttcatcgaa
      721 agatctctac atgtccccat acttccaagc cattgccgtc gacttcgatg gcacgataac
      781 cgaacttggg cgcccgagtg cccgcacgct cgccgcactg agcgaggttc gcgccgcggg
      841 agtgaaaacg ctgcttgtca ccggcagggt ggtcacggac ctcatgcacg tcttccctga
      901 tgcggagcaa tggttcgatg tgatcgtcgc ggagaatggc gcagtgattc gccgggatgg
      961 agtatcgcac gccctaacca gtcccgtgcc gctggagctc gacgaaccgt tggtggcaca
     1021 gggcgtctac ttccagcgcg ggcaagtgtt gctcgcctgc agcggcgatg acgagctcac
     1081 cgttctgagg gaggtgcggc gtctcgaagc ggactgtcag ttggtccgca atcgtgcagc
     1141 tctcatggtc ttaccctcgg aagtttcgaa aggttggggc ctcgccgaag ctttgaaagg
     1201 gctcggtgtc tcgtatcaca gcacgattgg gatcggtgat gcggagaacg acttgtcctt
     1261 gttccgccac tgcgagctcg gcgttgcggt cggcaacgcc gtcgagtcct tgaagcgaga
     1321 agcagacatc gtgctttccg ggagcgacgg cgttgccgtc gcggactttc ttcaaaatca
     1381 gttgctggtc gaggaaagtc ttcctccgtc gcgtcggtgg cgggtcacga tcgggctcct
     1441 ggacgatggc agcggggtga gcttaccggc atcccgagtc aacatcttgt tcgtgggaga
     1501 aagcggcgtg ggcaagtcct atgcagcagg gctcctcgca gaacggctga tcgagctcga
     1561 ctactcggtc tgcttcatcg acccagaggg agaccacgcg ccgctcgggc gccttcccaa
     1621 ggttctcacc gtgggcggtc gagggctatt gccggacgcg cgggacgttc ccaagttgat
     1681 gagccagcaa ctcggaagcg tgatcgtcga cctctccttt cttgctgaag cggagcgcac
     1741 gccatacacg gccgagctgc tccgcgcgct tcgcgaagag cggagcaact cgtgtttgcc
     1801 ccattggatc atgatcgacg aagcgcacat gccgtttggt gcaggtggtg aggtctgtgg
     1861 aatgttgggc ggccaaaagg ggctttgctt ggtgacctac gacgcgaccc ggctttgtcg
     1921 ctcatcgggg ctcgcgttcg acttcttggt tgccatcccc gggcaagaag ggttggatcc
     1981 agcagcggca gagagcatcc gtgagctatc tggcatcgac cctttcgggg aattttcgga
     2041 cccgaaacag ggcgaagcgt ggctcgtgcg actcgacggt cctccacgcg cagagcgctt
     2101 cagactgggc cggcgcttcg tgcgacacgt gcgccactgg cacaagtatg ccgaggctcg
     2161 ccttcctccc gctagggtgt tccgctttcg ctcgttctcc gggccctcgg gcgctcaagc
     2221 cgacaatctt tctgcatttc gccgggagtt actcttgtgc gattcgaagg ttctgcagca
     2281 ccacgccgcc aaccacgatt tctcgcgctg gctggagcat gcaataagcg acgagaccct
     2341 ggcgcgcgtc gcgcatgagc tcgagaaacg aagcgcggta gacgcgtcgt tcgaagacct
     2401 tcgtcgtggg ctggtcgagg cgatcgagga tcgctatc
//
LOCUS       DASRZF010000367         2742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1310855, whole genome shotgun sequence.
ACCESSION   DASRZF010000367 DASRZF010000000
VERSION     DASRZF010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310855"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="VFG22_16365"
     CDS             <1..601
                     /locus_tag="VFG22_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_274671342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3641 domain-containing protein"
                     /protein_id="HET6417872.1"
                     /translation="AEHRVELICSLPHYQSACTDVQRGDGVFEKSIRALRRLNELGYG
                     SGDSGLRLVLVTNPVGALLPAAQACLEVEWKRELERLYGITFDALYTITNMPISRHLE
                     WLLETGNLEGYMRSLVESFNPQAAHGLMCRSTISVGWDGRLYDCDFNQMLEISVSQDS
                     PGHVRDFDLSALEARTVQTDRHCFGCTAGTGSSCNGTTA"
     gene            699..1367
                     /locus_tag="VFG22_16370"
     CDS             699..1367
                     /locus_tag="VFG22_16370"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HET6417873.1"
                     /translation="MPDALAPTVTVDSDHAAVVSFAKESSRGARDARERAVKLYYAVR
                     DGIRYDPYSVLLTVDGLKASTTIERGRAWCVPKAVLLAAGCRALGIPARLGFADVKNH
                     LSTQRLRDTMKSDVFYWHGYTAIQLGGRWVKATPAFNIELCAKFRIKPLEFDGSEDSI
                     YHPFDLEGRRHMEYLRFHDEYDDVPIEEIMASYTKHYSGTVYDTPNGDFEREVDEETR
                     DQTC"
     gene            complement(1364..2296)
                     /locus_tag="VFG22_16375"
     CDS             complement(1364..2296)
                     /locus_tag="VFG22_16375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417874.1"
                     /translation="MLWPRTFGGSIRAGVPDVRAEIQDWCTREGIPRPSDALIADFYL
                     PMTNWLAEHVEEQTLVFGLSGAQGTGKSTLARLLQRLLERLHGLRAAILSLDDIYLTG
                     AERLQRAKTVHPLLVTRGVPGTHDVDLGLKVLRCLREGRPIALPRFDKAKDARVRVAD
                     WEPWEGRCDLVIFEGWCMGARPQRADELQTPVNDLERVEDPSGDWRWYVNRELGGKYQ
                     TLFAQLDLLVMLRPPDFDHVYAWREEQEARLRAAEAGTEVMTSDQVRRFVMHFERLTR
                     FMWEEMPGRADAVVLLGEDHLPERVSLGDATEGR"
     gene            2355..>2742
                     /locus_tag="VFG22_16380"
     CDS             2355..>2742
                     /locus_tag="VFG22_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HET6417875.1"
                     /translation="MPDPKIRILVAKPGLDGHDRGAKVVARALRDAGFEVVYTGLHQT
                     PDMIAVAAVQEDVDAVGLSIMSGAHNTLFPAVLDALSARGADDILVFGGGIVPEGDAK
                     ALLAKGVGAIFTPGAPLEEIVAWIRGN"
BASE COUNT          526 a          882 c          831 g          503 t
ORIGIN      
        1 cgccgagcac cgcgtcgagc tcatctgctc gttgccgcac tatcaatcgg cttgcaccga
       61 cgtccagcgc ggcgatggcg tcttcgagaa gagcattcgc gcgctgcggc ggctcaatga
      121 attgggttat ggatccgggg attcagggct tcgcctggtg ctggtgacca accccgtcgg
      181 cgctcttctt ccggccgcgc aggcctgcct ggaagtggaa tggaagcgcg agctggagcg
      241 cctttacggc atcacgttcg acgcactcta cacgatcacg aacatgccga tcagtcgaca
      301 cctcgaatgg ctgctcgaaa cgggaaacct cgagggttac atgcggagct tggtcgaatc
      361 gttcaatccg caggccgccc acgggctgat gtgccgcagc acgatctcgg ttggctggga
      421 tggtcgtctt tacgactgcg acttcaacca gatgctcgag atctccgtgt cgcaggattc
      481 gccggggcac gttcgggatt tcgacctcag cgcgctcgaa gcaagaacgg ttcagaccga
      541 ccggcactgc tttggatgta ccgcaggcac cggctcgagc tgcaacggca ccaccgcctg
      601 agcccaagag cggccatggc tctgccggcc gcggaaagcg aaccgatcga agccgcgccg
      661 acgtgctctt ccgtgtagga tacatcagaa tctgccgcat gcctgacgct ttggcgccca
      721 ccgtgacggt cgactccgac cacgctgccg tagtctcctt cgcgaaggag agctctagag
      781 gggcgcgtga cgcccgggaa cgcgcggtga agctctacta tgcggtgcgt gacggtatcc
      841 gttacgaccc ctactcggtg ctcctgacgg tcgacgggct caaagccagc accacgatcg
      901 agcgagggcg cgcctggtgt gtgcccaagg ccgttcttct cgccgctggc tgcagggcac
      961 tcggaattcc cgcgcgcctc ggattcgccg acgtgaagaa ccacctgtcc acccagaggc
     1021 tgcgcgacac catgaagagc gatgtgttct actggcatgg atacacggct atccaactgg
     1081 gcggaagatg ggtgaaggcc acgcctgcat tcaatatcga gctctgcgca aagttccgaa
     1141 tcaagccgct cgagttcgat gggtccgaag attcgatcta ccatccgttc gatctcgaag
     1201 gccggcgcca catggaatac ctccgcttcc acgacgagta cgacgatgta cccatcgagg
     1261 agatcatggc gtcatacacg aagcactatt cgggaacggt atacgatacg cctaacggtg
     1321 atttcgagcg cgaggtggac gaagaaacac gtgaccagac gtgctagcga ccttcggtcg
     1381 cgtctccgag gctgactcgc tcgggcaaat ggtcttcccc gaggagcacg accgcatccg
     1441 cgcgaccggg catctcctcc cacatgaagc gagtgaggcg ctcgaagtgc atcacgaagc
     1501 gacgcacttg atcggacgtc atcacctcgg ttccagcctc cgcggcacgc aatcgagctt
     1561 cctgctcttc gcgccatgca tagacgtgat cgaaatccgg gggtctgagc atcacgagca
     1621 ggtcgagttg agcaaagagc gtctgatact tgccgccgag ctcccgattc acgtaccagc
     1681 gccagtcccc gctcggatcc tccacgcgct cgagatcgtt gaccggggtt tgtagctcgt
     1741 cagccctttg gggccgcgcg cccatgcacc agccctcgaa gatcaccaga tcgcagcggc
     1801 cctcccaggg ttcccagtct gccacccgga ctcgcgcgtc tttggctttg tcgaagcgtg
     1861 gcagggcgat cggtctgcct tcgcgcaagc agcgaagcac cttcagtccg aggtcgacgt
     1921 catgtgtgcc gggcacccct cgggtgacca gcaggggatg aacggttttt gcgcgctgaa
     1981 ggcgctcggc gccagtgagg tagatgtcgt cgagtgacag gatcgctgca cgcaatccgt
     2041 gtagacgttc caagaggcgc tgaagcagac gggccagggt cgacttcccc gtgccttgcg
     2101 cgccgctgag cccaaacacc agcgtctgtt cctccacgtg ttccgcgagc cagttggtca
     2161 tcgggaggta gaaatccgcg atcaacgcat ccgacggtct gggaatccct tcgcgcgtgc
     2221 accagtcctg tatctccgcc cgcacgtcgg gaacaccggc acgaatgctg ccaccaaagg
     2281 ttcgaggcca aagcactccg cgagcatacc actggcactg gcaccgcgag tgcccctgga
     2341 ctaaaccttc gccgatgcca gaccccaaga ttcgaatcct agtcgcgaag cctggtctcg
     2401 atggccatga ccgcggcgca aaggtcgtgg ctcgtgcgct ccgcgacgcg ggtttcgaag
     2461 tcgtgtacac gggactacat cagacgcctg acatgatcgc cgtagcggcc gtacaagaag
     2521 acgtcgacgc cgtgggtctc tccatcatgt ccggcgcaca caacaccttg ttccccgcgg
     2581 tcctcgatgc gctctccgct cgcggagccg acgacatcct cgtctttggc ggtggcatcg
     2641 tccccgaggg ggacgccaaa gcgctactcg cgaaaggtgt gggcgcgata tttacgccgg
     2701 gcgcaccgct cgaagagatc gtcgcctgga ttcgcggcaa tg
//
LOCUS       DASRZF010000368         6012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_972652, whole genome shotgun sequence.
ACCESSION   DASRZF010000368 DASRZF010000000
VERSION     DASRZF010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 6012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6012
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972652"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VFG22_16385"
     CDS             complement(<1..287)
                     /locus_tag="VFG22_16385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417876.1"
                     /translation="MKRSDNFTPVRQATPLSTPSRWAILLYGTVCYAGFHAVFLWMVM
                     FLNDIPILPSVNSGTTRHWAAAIAINLGLVLLFGLQHSVMARRSFKKIWTQ"
     gene            complement(379..1761)
                     /locus_tag="VFG22_16390"
     CDS             complement(379..1761)
                     /locus_tag="VFG22_16390"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6417877.1"
                     /translation="MPSIRFCEATGRERLAYAIDGEGPTILFPAWWISHAEKDWEHDE
                     FREFFSALAQQHCVVRYDRVGVGLSDRIRETRDMTVEREVADLECLIDHLGLDEVTLF
                     AVSCGGPTAVTYAVRHPTRVRNIIFHGSYLRGCTIGNPEVRAAMLGLVRASWGLGSKT
                     LASIFTPDLSPEAIRRTTRMQRDAADSETAAQLLSLTYSGDVASIAPRVRVPCLVLHR
                     HDDSAIPFASGRELAANLPGACLAPLEGTAHPPWEGDTGPVLNAIFAFLGTGERSQTA
                     TQTSDSERNALLRQGDVWTLTFDAVQTHLKHSKGLADLAALLAAPGHEIAAAALMHGI
                     EQATPPGGSDEVLDERARNEYRARLQAIEEDLEEAQSFNDLGRIQRLEREHEAIFAEL
                     RAATGLGGRSRKLKDPGERARKAVTARIRESIQRIAEAHPAAGEHFTEAIRTGSFCCY
                     EPKTAVDWRV"
     gene            1959..2495
                     /locus_tag="VFG22_16395"
     CDS             1959..2495
                     /locus_tag="VFG22_16395"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET6417878.1"
                     /translation="MNQRRGALIRTYRKQPERAMIQDGARTAQTRYAPSDPLHSDVEI
                     GLGYGVRTPVGVHRGLGGLHDFPNPGDILCSALAACADSTIRQIAGQLHVEILTLEVS
                     VAGDIDLRGTLCVSQEVPVGFQHMSVSVRLELGPDTPPRMKDKLLAASEHCCVVLRTL
                     RQGVPVTIELDTAGDGEG"
     gene            complement(2570..4000)
                     /locus_tag="VFG22_16400"
     CDS             complement(2570..4000)
                     /locus_tag="VFG22_16400"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET6417879.1"
                     /translation="MRPSTNITPHTNQPSRRDSEVRPGDFGAPRPPRLSGAPVLGNLA
                     EFLSDPYGMFIRGYRSLGPVFSVRFLHRPMLVLAGAEGLRFLSKQGRELLESKPVWQE
                     FVTLFGGERALVSVDGELHAELRKRLRPSMSRSTLENRLPEVREVVQRCLRRWDGQTV
                     DVERFTRDLIFHELAALMDMAGIDPDEYYEDLLRVMHTSIEVTVGRRWPRFLLWNPRF
                     RAAKQRIMNMGIDLFRSVGQSNPEDGLFPILRRCVQDGLLGERDIPLLAMTPYFAGID
                     TVASTLSFALSAVYRRPDLQTALRKELEAASADSPTGREWLDRLKLAEAVKLEVSRRY
                     PVTLTMVRFARSPFVYAGRQVRAGDQLLISVGAQSLMHEYFEDPERFDPMRFVGPDAL
                     RPEAGTLNPYGMGPHTCLGAALADVQVIATLALLLELADIELIDPERPLEMKYTPYPL
                     PKGDRLRIRMRNRRRIAKKVTPTSEH"
     gene            4120..4572
                     /locus_tag="VFG22_16405"
     CDS             4120..4572
                     /locus_tag="VFG22_16405"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HET6417880.1"
                     /translation="MIPDESVAYALAAWKRFDDSVSDGLLRAVAGAFVLVAAADGELS
                     RSEADRFFDAVRSKRDVLAPIDFTALSKTFQDLCAAMFTDPEDGTRLALECVARVKGV
                     PQHAELVNGAAEIAAAADGRVASIEASVLQTIRRALGLEAPEEPTQLP"
     gene            4642..5649
                     /locus_tag="VFG22_16410"
     CDS             4642..5649
                     /locus_tag="VFG22_16410"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HET6417881.1"
                     /translation="MKAMIVREAGGPFTLDEREIPEPGPGEIRIKVEACGICHSDAFV
                     KFGQFPGLELPRAPGHEVAGVVDALGEGVDSFTVGHRVGVGWHGGHCFKCNPCRRGDF
                     INCTSAKIAGISYDGGYQEYMVCPWEAAARVPDELSPAEAAPLLCAGITTYNALRNSG
                     ARPGDLVAVQGVGGLGHLAIQFSNRMGFRTVAVSRGKDKEKLARDLGAHEYIDSQSTD
                     EVAELQKLGGANLILATAPNGKAIEGIVNGLHGRGKLMLVAAAGDPISISAFAMLSGK
                     LIHGWPSGTAVDSEDTMSFAALANVRPKIERFPLEAANEAFAKVMENTIRFRAVLDMA
                     I"
     gene            5666..>6012
                     /locus_tag="VFG22_16415"
     CDS             5666..>6012
                     /locus_tag="VFG22_16415"
                     /inference="COORDINATES: protein motif:HMM:NF016879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Coq4 family protein"
                     /protein_id="HET6417882.1"
                     /translation="MSVTSMRPIAAVRAIRLVFADPDATEHVFEVIDALQGPNLKRIR
                     ARLRRSDIGSRLLETKPNLLPLLSDRDALRALPEGSLGRAYLAFVESEGISADGLVEA
                     SVGARKSGIDGEFL"
BASE COUNT         1092 a         1798 c         1981 g         1141 t
ORIGIN      
        1 atctgggtcc agatcttctt gaatgagcgc cgtgccatca cgctgtgctg cagaccgaag
       61 agcaggacga ggccgaggtt gattgcgatg gcggcggccc agtgacgcgt cgtgcccgag
      121 ttgacggacg gaaggatcgg aatgtcgttg aggaacatca ccatccagag gaagacagca
      181 tggaaccctg cataacagac ggtcccgtag agcaatatgg cccagcggct gggcgtcgag
      241 agcggggtgg cttggcggac tggggtgaaa ttgtcactgc gcttcatggc gttctccttg
      301 gtggcttgga cggtcgtcgt ccttgcccaa gaggcgccag ggcgcgtcgc gcagcccgct
      361 agaccattcc tagcggggtc agacccgcca atcgacagcc gttttcggct cgtagcagca
      421 gaatgagccc gtacggatcg cctcggtgaa gtgttcgccg gcagcggggt gggcctcggc
      481 gatgcgctgg atactctcgc ggattcgtgc agtcaccgcc ttgcgcgcgc gttcgcctgg
      541 atccttgagc ttgcggcttc ggcctcccag cccggtcgcc gcacgaagct cggcgaagat
      601 cgcctcgtgc tccctttcga gacgctggat tcgcccgagg tcgttgaagg actgcgcctc
      661 ctcgaggtcc tcctcgatgg cctgcaagcg tgcgcggtac tcgttccgcg cacgttcatc
      721 caaaacctca tcactaccgc cgggcggcgt tgcttgctcg attccatgca tgagcgcagc
      781 ggccgcgatt tcgtgtcccg gagctgccag aagggcggca aggtctgcga gacctttgct
      841 gtgcttgaga tgtgtctgaa cggcgtcaaa tgtcagcgtc cagacgtcgc cttggcgcag
      901 aagtgcgttc cgctccgagt cactggtttg cgtcgcagtt tgagatcttt cgcccgtgcc
      961 gaggaaggcg aagatcgcgt tcagtacggg gccggtgtcg ccttcccacg gtggatgggc
     1021 cgtgccctcg agcggcgcaa gacacgcgcc cgggaggttg gcggcgagct cccgcccgga
     1081 ggcgaacgga atggcgctgt cgtcgtgtcg gtgcagcacg aggcatggca ctcgaacccg
     1141 aggcgcaatc gacgcgacat cgcccgagta ggtgagcgat agcagctgcg ccgcagtctc
     1201 gctgtctgcg gcatcccgct gcattctggt cgtacgccga attgcctcgg gtgagaggtc
     1261 tggcgtaaaa atcgaggcga gcgtcttcga gccaagtccc cagcttgctc gaacgaggcc
     1321 cagcatggct gcgcgcacct ccgggttgcc gatggtgcag ccccgaagat acgagccgtg
     1381 aaaaatgatg tttcgaaccc tggtcgggtg ccgaaccgcg taggtgaccg ccgttggtcc
     1441 gccgcacgag accgcgaaca gcgtgacctc gtcgagtccc aagtggtcga tgaggcactc
     1501 caaatcggct acctcgcgct cgacggtcat gtcgcgtgtt tcgcgtattc ggtcgctgag
     1561 ccctacgccc actcgatcgt agcgaaccac gcagtgctgc tgcgccagcg cgctgaagaa
     1621 ttcgcgaaat tcgtcgtgct cccagtcctt ctcggcgtga ctgatccacc atgccggaaa
     1681 caggatcgtc ggaccctcgc catcgatggc gtaggcgagg cgttctcggc cggtggcctc
     1741 gcaaaatcgg atacttggca cggcaggatc ttatccgtcc tgtcggcttc ggacaacggc
     1801 catgcgcgag ctagcggccg cttgttcggt tctggcgcct ccttggtgga gccgagctcc
     1861 gcaagaatca cgcaaagtgc acttacgcaa agtgccaact agaaaggcaa gccatgacat
     1921 ccgcacccgc tatcgcacca tcaagccccg gcgagctcat gaatcaacga cgcggcgcgc
     1981 tcatccggac ctatcggaag cagcccgaac gcgcgatgat tcaagacgga gcgcgcactg
     2041 cgcagactcg atacgcgcct agcgatccgc tgcacagcga tgtcgaaatc ggacttggat
     2101 acggcgtcag gacaccggtt ggtgttcacc gcggactcgg tggcctacac gatttcccaa
     2161 atcccggtga catattgtgc tctgcactag cggcatgcgc cgactcgacg attcgccaga
     2221 tcgcgggcca actccatgtc gagattctga cgctcgaggt ttcggtcgcc ggagatatcg
     2281 atctgcgcgg tacgctgtgc gtgtcgcagg aggtcccagt ggggtttcag cacatgagcg
     2341 tgagcgtacg actcgagctc ggtccagaca ctccgccacg aatgaaagac aagttgttgg
     2401 ccgcttcgga gcactgctgc gtggtcctga gaacgcttcg gcagggcgtg ccggtgacga
     2461 ttgagctcga cactgcgggt gacggcgaag gctaggattg gagccgggta agtggaattg
     2521 cggccgttgc cgagcttgca tggcggcgat gcgaagtcgc gtcttgttcc tagtgctcag
     2581 aggtcggagt gacctttttg gcgattcggc gccggtttcg cattcgaatg cgtagtcggt
     2641 cgcccttcgg cagtggatat ggcgtgtact tcatctcgag tggccgctcc gggtcgatca
     2701 gctcgatgtc tgcgagctcc agaagcaagg caagggttgc gatgacctgc acgtccgcca
     2761 gcgctgcacc gaggcaagta tgtgggccca tcccgtaggg gtttagggtc ccggcctcgg
     2821 gccgtagcgc gtcggggccc acgaaacgca tggggtcgaa gcgctccgga tcctcgaagt
     2881 actcgtgcat gaggctctgc gcgccgacac tgatcagcaa ttggtcgccc gcgcggacct
     2941 gccgcccagc atagacgaag ggtgagcgag cgaagcgaac catggtgagg gtgacgggat
     3001 atcgtcgcga cacctccagt ttcacggctt cagcgagctt caagcggtcg agccattcgc
     3061 gacccgtcgg cgaatcggcg cttgcggctt ctagctcttt gcgaagcgcg gtttgaaggt
     3121 cgggccggcg gtagaccgcg gacagcgcga agctcagggt ggaggcgacg gtatcgatcc
     3181 cggcgaagta gggtgtcatg gccaggagtg gaatgtcccg ttcgccgagc aagccgtcct
     3241 gaacgcaccg ccgcaggatg ggaaacaagc cgtcctccgg attgctttgc ccgaccgacc
     3301 gaaagaggtc gatgcccatg ttcatgatgc gctgctttgc ggcgcgaaac ctggggttcc
     3361 agagcaggaa ccgcggccaa cggcgaccga ctgtgacctc gatcgaggtg tgcatcacgc
     3421 gcagcaagtc ttcgtagtat tcgtccggat cgatgcccgc catgtccatg agcgcggcga
     3481 gctcgtgaaa aatcaggtcc ctggtgaagc gttcgacgtc gacggtttga ccgtcccagc
     3541 gccgcaagca ccgctgaacg acctcacgca cctcgggcag gcgattttca agggtgcttc
     3601 gcgacatgga cggacggagg cgcttgcgca gctcggcgtg taactcgccg tcgacgctga
     3661 ccagggcccg ctcgccccca aagagcgtga cgaactcctg ccagacgggc ttcgactcca
     3721 agagctcgcg accttgcttc gacaagaacc gtagaccttc ggcgcctgcc aagaccagca
     3781 ttgggcgatg caggaagcgc accgagaaca cgggacccag gctccggtag ccccggatga
     3841 acattccgta tgggtcgctg aggaactcgg ccaaattgcc gagcactgga gcgccagaca
     3901 gccggggcgg ccgcggcgcg ccgaaatcgc ccgggcggac ttccgaatcc cttcgcgatg
     3961 gctggttcgt gtgaggcgta atgttggtgc tcggacgcat gcttctctga ggctatagca
     4021 agcttagccg gagccggcgt tgcagagaag cacgtccacg tctcggatgc ttggccgcgc
     4081 ttcgagatcg ggttaggctc acaacctaga gagtgaccga tgatcccgga cgagtcagtc
     4141 gcatacgcac ttgcagcatg gaaacgcttt gacgacagtg tttcagatgg cctcttgcgt
     4201 gcagtcgcgg gggccttcgt tctggtcgca gccgctgatg gtgagctgag ccgttcggag
     4261 gcagatcgtt ttttcgatgc ggtgcggtcc aaacgggacg tccttgctcc gatcgatttc
     4321 acggcgcttt caaagacgtt ccaggatctt tgcgcggcga tgtttaccga tcctgaggac
     4381 ggtacgcgac tcgccctcga gtgcgtagca cgcgtgaagg gtgttcctca gcatgccgag
     4441 ctcgtgaacg gcgcagccga gatcgccgcc gcagctgatg gccgagtcgc gtcgattgag
     4501 gcgagcgtgc tgcagaccat tcgccgcgcc ttgggcctcg aagcccccga agaacccacg
     4561 cagttgccct gaggcaggcg tcggacgtgg tgattgccaa ggtttgctcg agccctatac
     4621 cgaacgctcg gaggctgaat catgaaggca atgatcgtac gagaggccgg cgggccgttt
     4681 accctggatg agcgcgagat ccccgaaccc gggcctggcg agattcgcat caaggtcgag
     4741 gcctgtggaa tctgccatag cgacgcgttc gtgaagttcg gacagttccc ggggctcgag
     4801 cttccgcgcg ctccagggca cgaggtggca ggcgtcgtgg atgcgcttgg agaaggtgtg
     4861 gactcgttta cggtcgggca ccgcgtcgga gtcggttggc acggtgggca ctgcttcaag
     4921 tgcaatccat gtcgacgcgg cgacttcatc aactgcacca gcgccaagat agccggaatc
     4981 agttacgacg gtggttacca ggagtacatg gtctgtccct gggaggcggc tgccagggtg
     5041 cccgatgagc tcagcccggc cgaagccgct cctctccttt gcgccggcat caccacgtac
     5101 aacgcgcttc gtaacagcgg cgctcgcccc ggcgatctgg tcgccgttca aggcgtgggt
     5161 gggctcggtc atctcgcgat tcagttttcg aatcgcatgg gctttcgcac cgttgctgtc
     5221 tcgcgcggga aggacaagga gaagctggcc cgagatttgg gggctcacga gtacatcgac
     5281 tcccagtcga cggacgaggt cgctgagctc cagaagcttg gcggtgcgaa cttgattttg
     5341 gcaacggcgc caaacggcaa ggcgatcgag ggcatcgtca atggccttca tggccgagga
     5401 aagctcatgc tcgttgcggc ggccggggat ccgatctcga tcagcgcctt tgcgatgctc
     5461 tccgggaagt tgatccacgg ttggccgagc gggacggcgg tcgacagcga agacacgatg
     5521 agcttcgccg cgctcgcaaa cgtgcggccc aagatcgaac gcttcccgct cgaggccgcc
     5581 aatgaggctt tcgccaaggt catggagaac acgatccgct tccgcgcagt gctcgacatg
     5641 gcgatttgat gctaaagagc ggttcatgag tgtgacttcc atgcgtccca tcgcggcggt
     5701 tcgcgcgatt cggcttgtgt ttgccgaccc ggatgccacg gagcatgtct tcgaggtcat
     5761 cgacgcattg cagggaccga acctcaagcg gatccgcgca cggcttcgcc gaagcgacat
     5821 cggtagccgg ctgctcgaga cgaagcccaa tctgcttccg ctgctcagcg accgggatgc
     5881 gcttcgtgcg ttgcccgagg gcagccttgg acgagcttac ttggccttcg tcgagtcgga
     5941 gggcatcagt gcggatgggc tcgtcgaggc ctcggtagga gcgcgcaaga gcggtatcga
     6001 tggcgagttc ct
//
LOCUS       DASRZF010000369         2995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3717141, whole genome shotgun sequence.
ACCESSION   DASRZF010000369 DASRZF010000000
VERSION     DASRZF010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3717141"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="VFG22_16420"
     CDS             <1..912
                     /locus_tag="VFG22_16420"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET6417883.1"
                     /translation="LESYAAQFELPVLNGVRVDRLSRNDGHYQLEAGSRVFRAEQVIV
                     AMSNYQRPSVPAFAKHLDAKIVQLHSMHYKAPSQLDPGPVLLVGAGNSGSELAMELSK
                     SRHVYMAGRSTGEIPFRVGGFLGRLLLARLVLRVLFHRILTVGTPIGRKLRPKLLHGG
                     GPLIRVKARDMAAAGVERTPRVTEVRNGQPVLKDGRALDVKNVIWCTGYETGFSWIDL
                     PILDEHAVPRHQAGLVADAPGLYFVGLHFLYSLSSEMIHGVGRDAERIAMLAHARLRE
                     GARSPAEISAADRAGLSAAMQASRPGC"
     gene            complement(875..1945)
                     /locus_tag="VFG22_16425"
     CDS             complement(875..1945)
                     /locus_tag="VFG22_16425"
                     /inference="COORDINATES: protein motif:HMM:NF018452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HET6417884.1"
                     /translation="MSKTHETFMQLLETLRELPETFTDEFKDLGPQSQLQGYRHLAHL
                     LSYGFELYLESDPLRPSFLPLARPTQKILGDNTDSVYCFTQARGDQRYRIWGNIGDAC
                     YLSFCAYAGEPDGSWSDSLAVNINHTQIDFDTDGSFEIFVGPAEPDAPNHFAIGARAV
                     CIISREYYFDRGKDRLAEIHIANTGSIEAPSPETDASLSTKLEAVTTFVTQTAAMIPP
                     PGTDEPNELGEPFGFDPDGMGWGTPDNIYAMGSFRLADDEVMVIEGRSPECCYWGVQT
                     WNHYLQSFDARYYQVSLNSKQVTLDPDGGWTIYVSKHDPGIGNWVGTAGHDEGIVFCR
                     WLLAETMPDRPSSRVVKLASLH"
     gene            complement(1942..>2995)
                     /locus_tag="VFG22_16430"
     CDS             complement(1942..>2995)
                     /locus_tag="VFG22_16430"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET6417885.1"
                     /translation="TEANLSLLGRLMVQGYTVDNLANRLRLVDWRKKHPEIANEEIVA
                     PLFIVGLPRTGTTILHALLEQDPANRSPLFWEVQFPVPPATPQTWDDDPRIEEDEKVL
                     RQLYRLVPGFEAMHPMAATMPQECVAVFTMCFMSEQLQVQFDVPSYQAWLDEQDMHST
                     YEFYKRFLQHIQSGGVRGKRLVLKSPAHLHLLDTLLDVFPDARIVHTHRDPLEVCASV
                     ASLTATLRGASTDAIDFKAIGRQQVAWWAKLIARSLEQRKRLAGRSEQFFDVKMSETV
                     ADPIDVVRRMYAHFGYALSAEVEAKMVAFMAENSRDKHGSHSYTPADFGIDLERDRGP
                     FEEYIDYFGLSETGEP"
BASE COUNT          556 a          894 c          939 g          606 t
ORIGIN      
        1 ctcgagtcgt acgccgctca gttcgagctc cccgtgttga acggagttcg cgtcgaccga
       61 ctgtcgagga acgacggtca ctaccagctc gaggccgggt ctcgcgtgtt tcgtgccgag
      121 caggtcatcg tcgcgatgag caactaccag cgaccgagtg ttcccgcgtt tgccaaacat
      181 ctcgacgcaa agatcgtgca actccactcc atgcattaca aggcgccgag ccagctcgac
      241 ccggggccag tgctgctcgt gggcgccggc aactccggct ccgagctcgc gatggagctc
      301 tccaagagcc gacacgtgta catggctgga cgcagcacgg gcgagattcc atttcgcgtg
      361 ggcgggtttc tcggccgtct ccttctcgcg cgcctggttc tccgagtgct gttccaccgg
      421 attctcaccg tgggcacccc gattgggcgc aagctccggc ccaagctact tcacggtgga
      481 gggccactga ttcgtgtcaa agcgcgcgac atggctgcgg ccggtgtcga gcgcacgccc
      541 cgcgtcactg aagtgcgcaa tggtcaaccc gtcttgaaag atggtcgcgc cctcgacgtg
      601 aagaacgtca tctggtgtac aggctatgaa acaggctttt cctggatcga tctcccgatc
      661 ctcgatgagc acgcggtgcc gcgccaccag gcaggcctgg tcgctgacgc accaggactt
      721 tacttcgtcg gcctgcactt tctctactcg ctatcctccg agatgattca cggcgtcggt
      781 cgcgacgcgg agcggatcgc catgctcgcg cacgctcgcc ttcgggaagg cgcccggtcg
      841 cctgcagaaa tttcggccgc ggaccgcgca ggcctcagtg cagcgatgca agcttcacga
      901 cccggctgct aggtctatcc ggcatggtct ccgccagaag ccagcggcag aagacaatgc
      961 cctcgtcgtg cccggcagtg cccacccagt tgccaatccc cgggtcgtgc ttggagacgt
     1021 agatcgtcca accgccatcc ggatcgaggg tgacctgctt cgagttgagc gaaacctggt
     1081 aatagcgcgc atcgaacgac tgcaagtagt ggttccaggt ttgaacaccc cagtagcagc
     1141 actccggtga gcggccctcg atgaccatga cttcgtcatc cgcaagtcgg aacgatccca
     1201 tcgcatagat gttgtcaggc gtgccccagc ccatgccatc cggatcgaag ccgaacggtt
     1261 cgccgagctc gttgggttcg tcggttccag gtggggggat catcgccgcg gtttgagtca
     1321 cgaaggtcgt gacggcttcg agcttagtgg agaggctggc gtcggtctcg ggggaaggcg
     1381 cttcgatgct ccccgtgttg gcgatgtgga tctcggcgag tcgatctttg ccacggtcga
     1441 agtagtactc gcgggagatg atgcacacgg cccgtgctcc tatcgcgaaa tggttgggag
     1501 cgtccggctc ggcgggaccg acgaagatct cgaagctgcc gtcggtgtcg aagtcgattt
     1561 gggtatgatt gatgttgact gcaaggctgt cggaccagga cccgtcgggc tcacccgcgt
     1621 acgcgcagaa gctcagatag caggcatccc cgatgttccc ccagatacga tagcgttgat
     1681 ccccgcgcgc ctgagtgaag cagtacaccg aatcggtatt gtcgccgagg atcttctggg
     1741 tcggtcgcgc aagcggtaag aacgaaggcc gcagcggatc gctctcgagg tagagctcga
     1801 aaccatagct cagtaaatgc gcgagatgcc gatagccttg aagctggctt tggggtccga
     1861 ggtccttgaa ttcgtcggtg aaggtttcgg gaagctcgcg gagcgtctcc agcaactgca
     1921 tgaaggtttc gtgtgtcttg ctcatggttc cccagtttcc gagaggccga agtagtcgat
     1981 gtattcctcg aacggtccgc ggtctcgctc gaggtcgatc ccgaagtctg ccggcgtgta
     2041 actgtgggag ccgtgcttgt cgcgactgtt ctcggccatg aatgcgacca tcttggcttc
     2101 gacctcggcg ctgagtgcgt agccgaagtg cgcatacatg cggcgaacga cgtcgatggg
     2161 gtctgcgacc gtctcgctca tcttcacgtc gaagaactgc tcgctgcgcc ctgcaagccg
     2221 cttgcgctgc tccagcgacc tcgcgatgag cttggcccac catgcgacct gctgccggcc
     2281 gatcgctttg aaatcgattg cgtccgtgga tgcgccgcga agtgtggcgg tgaggctcgc
     2341 caccgacgca cagacctcga ggggatcacg atgcgtatga acgatgcgcg catcgggaaa
     2401 cacgtcgagg agcgtgtcta gcaagtgaag gtgtgccggt gacttcaaga ccaacctttt
     2461 gcctcgtacg ccgcccgatt gaatgtgctg aaggaaacgt ttgtagaact cgtaggtcga
     2521 gtgcatgtcc tgttcgtcga gccaagcctg atacgaagga acatcaaact ggacctgtag
     2581 ctgctccgac atgaagcaca tcgtaaaaac cgctacgcat tcctgaggca tcgtcgcagc
     2641 cattggatgc atcgcctcga agccgggaac caggcgatag agctgccgca gaaccttttc
     2701 gtcctcttcg atccgtggat cgtcgtccca ggtttggggg gttgcgggtg gcacgggaaa
     2761 ctgaacttcc cagaacagcg gagagcgatt ggccgggtct tgttcgagca gggcgtgaag
     2821 gatggtggtg cccgttcgcg gaaggccgac gatgaacaac ggcgcgacga tctcctcgtt
     2881 cgcgatctcg gggtgcttct tgcgccagtc gaccagccgc agtcgattcg cgagattgtc
     2941 gaccgtgtat ccctgaacca tcagccggcc gagcagagag agattcgcct cggtc
//
LOCUS       DASRZF010000370         2209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1312504, whole genome shotgun sequence.
ACCESSION   DASRZF010000370 DASRZF010000000
VERSION     DASRZF010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1312504"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1426
                     /locus_tag="VFG22_16435"
     CDS             <1..1426
                     /locus_tag="VFG22_16435"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6417886.1"
                     /translation="GISREEQQKIFDRFHQTETSERRKQGGVGIGLALARELAQLHGG
                     SLTVDSVVGEGSTFTLFLLSGKEHFHTEIMERRQLQTDEHPGRRVEDHSTGTHTMEME
                     ATTVELRQSQRPPERLLLDRGRLPRVLVAEDEADLRGFIVGVLKSSYEVDAAANGAEA
                     LELMKANRPDLVLTDVMMPGTSGLDLTRAIKEDPSLRHIPVILLTARGESDAALEGYD
                     AGADDFVSKPFHTKVLQARIRAHLKMRGLSLQLADQARLASAGTLAAGLAHEVKNPLN
                     AAVNAVRVLAKGGSSRVSNEKLMTLIIEALNRIDGVVSALDAHARPADGTDLAPCNVR
                     KAVESTLNLLEHKMKHVTVHEDYEVTGDVFAPARAFNQVLLNLIDNSIRSEAKNVWIE
                     LRQVDKQISVAVADDGPGVPADVVHRIFDPFFTTRAEGQGTGLGLHLSRRIAQDCGGE
                     LRYEPRPGGGARFVMEIPAMEQAA"
     gene            1423..>2209
                     /locus_tag="VFG22_16440"
     CDS             1423..>2209
                     /locus_tag="VFG22_16440"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6417887.1"
                     /translation="MTAHVLFVDDEEPNLVVFEAVCGDDFPVLTASSAADGLLLMKEH
                     EIGVVLTDQRMPGMTGIELLEKVEIEYPETIRLLITAYSDLQAAEDAINRGHVRRYMR
                     KPWEPEVLRAELRDAIDLYNLNTRVKELEQRLLDTERAYSLSVVATGLADELRNPVGW
                     IRNNLTVIETSISAATAALEEKTPNVARARGTLAQVENAVLDARDGVKRILELTEEMA
                     KAPDEAVAEVVDLADAVRVALRKAESSGLRGRALIEVTTGVEPK"
BASE COUNT          454 a          637 c          728 g          390 t
ORIGIN      
        1 cgggatttcg cgagaagagc agcagaagat cttcgaccgc tttcatcaga cggagacttc
       61 ggaacggcgc aagcagggcg gcgtgggtat tggacttgcg ctggctcgcg agcttgccca
      121 gctccacggt ggctccctca ccgtggacag cgtcgtcgga gaggggtcga cattcacgct
      181 ctttctgctg agcggcaagg agcactttca cacagagatc atggagcggc gtcagcttca
      241 aaccgatgag catccagggc gccgagtcga agaccacagc accggaacgc ataccatgga
      301 gatggaggct acgacggtcg agcttcggca gagtcagcgt ccacccgagc gactcctgct
      361 tgatcgtgga cggcttccgc gggtgctggt agctgaagac gaagctgatc ttcgcggatt
      421 catcgtcggc gtcttgaaga gcagctacga ggtcgacgcc gcggcgaacg gcgctgaggc
      481 gcttgaattg atgaaggcga acagaccaga cctcgtgctg accgatgtga tgatgccggg
      541 cacaagtgga ctcgatctca cccgtgcgat caaagaggat ccgtcgcttc gccatatccc
      601 ggtgatcttg ctcacggccc gcggcgagag cgacgctgcg ctcgaaggtt acgacgcagg
      661 cgcagacgat ttcgtctcga agcccttcca cacgaaggtg cttcaagcgc gcattcgtgc
      721 acacctcaag atgcgaggtc tgagcttgca gcttgcggat caagcgcgcc tggcttccgc
      781 gggcacgctc gcggccggac tcgcgcacga ggtgaaaaac ccgctcaacg cagccgtgaa
      841 cgccgtgcga gtcctcgcaa aaggtggctc ttcacgggtt tcaaacgaaa agctgatgac
      901 gttgatcatc gaggcgctca atcgtatcga tggcgtcgtc tccgccctcg acgcgcatgc
      961 gcgcccggcg gatgggaccg atctggcgcc ctgcaacgta cgcaaggccg tcgagtccac
     1021 cctcaacctg ctcgagcaca agatgaagca cgtcacggtg cacgaagact acgaagtcac
     1081 cggcgacgtg ttcgcacctg cacgggcgtt caatcaagtg ctgctcaact tgatcgacaa
     1141 ctccatacgg tcagaggcga agaacgtctg gatcgagctg aggcaggtgg ataagcagat
     1201 ttctgtggcc gtggccgacg atgggcccgg cgttccagcc gacgtggttc atcgcatttt
     1261 cgaccccttc tttacaacca gagcggaagg tcaagggacc gggctcgggc tgcacctgag
     1321 ccgaagaatc gcgcaggact gtggtggtga gctaagatat gagcctaggc ccggtggggg
     1381 ggcccgcttc gtgatggaga ttcccgcgat ggagcaggcg gcatgacagc gcacgtacta
     1441 ttcgtcgacg acgaggagcc gaatctggtc gtcttcgagg cggtctgcgg cgatgacttt
     1501 cccgttttga ccgcgtcgag cgcggccgac ggtttgctat tgatgaagga gcacgagatc
     1561 ggagtcgtgc tcaccgacca gcgcatgccg ggcatgacgg ggatcgagct gctcgagaag
     1621 gtcgagattg agtatccgga aacgatccgg ctactgatca cggcgtactc ggacttgcag
     1681 gcggccgagg atgcgatcaa ccgcggccat gtccggcgct acatgcgtaa gccctgggag
     1741 cccgaggtct tgcgagccga gcttcgcgat gcgatcgatc tctacaacct caacacacgc
     1801 gtcaaagagc tggagcagcg cctgctcgat acagagcgcg cgtattcatt gagtgtggtg
     1861 gcaactgggc ttgccgacga gctacggaat ccggtgggtt ggattcgcaa caacctcacc
     1921 gtgatcgaga cttcgatctc ggcggcgaca gcggcgctcg aagagaagac acccaacgtc
     1981 gcgcgcgccc gtggaacgct ggcgcaggtc gagaacgctg tgctcgatgc gcgtgatggc
     2041 gtgaaacgga ttttggagct caccgaggaa atggcgaagg cgcccgacga ggccgtcgcc
     2101 gaggtggtcg atctcgcgga cgcggtgcgc gtggcgcttc gaaaggcgga gagcagcggg
     2161 cttcgtggtc gcgcgctgat cgaagtgacc accggtgtcg aacccaaag
//
LOCUS       DASRZF010000371         4426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3381533, whole genome shotgun sequence.
ACCESSION   DASRZF010000371 DASRZF010000000
VERSION     DASRZF010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3381533"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..928
                     /locus_tag="VFG22_16445"
     CDS             77..928
                     /locus_tag="VFG22_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET6417888.1"
                     /translation="MGLKNTVDWVRTEWGIFRKGGDMGWSERGRWVRQWIPFGTRTIG
                     WGLLSITVGPLTKGSASSWAAKRWSQSSAKGLHIYIEASGEENVPEGGFVYASNHESL
                     IDILVLGAALPGDFKWAAKRSVMNIPFLGWHLRLAGHVPVDRNGRKDSAAAVTEAFES
                     VLRNEHPLLVFPEGTRTEDGKLRPFKNGAFQAAVLTGKPVVPVALKGTFTLMSREAVD
                     TGSSKDPEDRLVTVRIGEPLYPRVELDETEAVIELRDRTRAAVLEMLAATTDAKAAAA
                     RAQPLRS"
     gene            complement(888..1331)
                     /locus_tag="VFG22_16450"
     CDS             complement(888..1331)
                     /locus_tag="VFG22_16450"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HET6417889.1"
                     /translation="MQYFEDIQVGDAKESTEDYELSAEEIIEFCKKWDPLPFHVDERA
                     AAATPVGKLFTSAIHSVAIAIRLGHQMQNEPTATVMGLGWDRVRFHTPACVGDRLRVR
                     GEIIETRPSESKPGLGIIRSLLTLSNQRGETVVSFEAAALLRQRP"
     gene            complement(1332..1766)
                     /locus_tag="VFG22_16455"
     CDS             complement(1332..1766)
                     /locus_tag="VFG22_16455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417890.1"
                     /translation="MNLNDVLSDEAKRASIIEDVCRLVDEEVAKQKGISGVAVKAGYR
                     LVQGVKPGFVRNVVKALLPDFAAALEPISAQAVSSGQTVSQYFARNTGEVAEALLAVT
                     DSRAQSSEHGSVKGAYGKLRSSARKNVESAVPGLGKIIEKYV"
     gene            complement(1741..2859)
                     /locus_tag="VFG22_16460"
     CDS             complement(1741..2859)
                     /locus_tag="VFG22_16460"
                     /inference="COORDINATES: protein motif:HMM:NF014177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HET6417891.1"
                     /translation="MIKYIGSKRLLVPHILKAVSAFPESGRVLDLFSGTSRVARGLKA
                     TGRYVIANDHLSYASTLARCYVQADANRWLEDAAQLIAELSELAPDAGYFTKAFCEDA
                     RYFQPRNGARVDAIREEIRRRSLQPELEAIALVSLMEAADRVDSTTGVQMAYLKQWAA
                     RAFKEIELRVPKILPGPGQALNLDAVEAAAEVEADIAYIDPPYNQHSYMGNYHVWESL
                     VRWDKPETYGIANKRIQCKEYKSDFNSKRRIRQGLQALFDAVRCKHMLVSFNNEGHVD
                     REEMTALLSTRGHVGVVAVDFKRYVGAQIGIHNPSGEKVGRVSHLRNKEYLFVVSERR
                     EIVDSVVVAIESSIAAASLASGPAKGKRMDHESERRVE"
     gene            complement(2856..>4426)
                     /locus_tag="VFG22_16465"
     CDS             complement(2856..>4426)
                     /locus_tag="VFG22_16465"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6417892.1"
                     /translation="RLGSNWARSAHRLMSRHDPTEVTRRHVFSEKSLSKKEEQARIVV
                     LAGTLAGDKYAIDKELTLGRGDNASVKLDDTLVSRIHARIYQHENGSYVIEDLESRNG
                     TQVNGMAVTRRVLTYGDRIQIGSSLLLFAHHDPMEQQVAQRRKLEAIGRLGTGVAHDF
                     NNLLGAVRSSIDFLNDLPGETKLSDPDVREAFNDIRSAAATATEMTSRLLGFAREVER
                     SHTEVDLSELCHHVVQLARHTFHRGIRIDARIDRGLKVSGDRGQLHQLLMNLLINARD
                     AMPNGGKLKISAKKADPVDLEKAPLNPLVAHAIITVEDTGIGMDDATRERAFDPFFTT
                     KGDQVGAGLGLAAVYEIAKSHNGHITVHSVPDRGSEFQVVFPLTSERPMIPTEPSLPA
                     IKLPATQLLIMLVDDEDVVRRSAGRVVRQSGHRVIFARSGDEALRIYTETQPRPDLVL
                     MDLNMPGMTGDQAFKKLKEIDPDAPVVFVSGYWDRDLERELRSMGALGFVQKPYEAAT
                     LRDAIVQAMTSQTS"
BASE COUNT          829 a         1362 c         1341 g          894 t
ORIGIN      
        1 gagtcccgtg cgtgcgatgg cctgagcaag gtcaggcgct tttcatgcgc ccgtcggggt
       61 ggtagaaggg ccgaggatgg ggctgaagaa caccgtcgac tgggtgcgca ccgaatgggg
      121 catcttccgc aagggcggag acatgggctg gagcgagcgt ggacgctggg tccggcagtg
      181 gattccattc ggcacccgaa cgatcggctg ggggctgctt tcaatcacgg taggcccgct
      241 caccaagggt tcggcctcca gctgggctgc caagaggtgg agccaatcct ctgcaaaggg
      301 actccacatc tacatcgagg caagcggcga agagaacgtt ccggaaggcg gtttcgtcta
      361 cgcgagcaat cacgaaagcc taatcgacat actcgtacta ggcgcggcgc tgcccggcga
      421 tttcaaatgg gccgcaaagc gttcggtgat gaacatcccc ttcctcggtt ggcacctgcg
      481 cttggcagga cacgtgccgg tcgatcgaaa cgggaggaag gattccgccg cggcagtcac
      541 cgaagcattc gaatccgtgc tccgaaacga gcatccattg ctcgtcttcc cggagggcac
      601 ccgcaccgaa gacggcaagc tgaggccgtt caagaatggc gcattccaag ccgccgtctt
      661 gacgggcaag cccgtggtgc cggttgccct caaaggcacg ttcacactga tgtcacgtga
      721 agcggtggac acgggctcga gcaaggaccc cgaggatcgg ctcgtgacag tgcgaatcgg
      781 cgagccgctc tatccgcgtg tggagctcga cgagaccgaa gctgtaatcg aactacggga
      841 tcgcacccgt gcagcggtgc tcgagatgct cgcggccacg acggacgcta aggccgctgc
      901 cgcaagagcg cagccgcttc gaagctaact acggtttctc cgcgctggtt gctgagggtc
      961 agaaggcttc tgatgatgcc gagccccggc ttgctttcgg aaggccgggt ctcgatgatc
     1021 tcacctcgaa cccgcagccg atcgcccacg caggcaggcg tatgaaagcg gactcgatcc
     1081 caaccaagcc ccatcacggt ggccgtcggt tcgttctgca tctggtgccc aagacgaatc
     1141 gcgattgcga ccgagtgaat cgcggaggtg aaaagcttcc cgaccggcgt cgctgcagcc
     1201 gctcgctcat cgacgtggaa cgggagcgga tcccacttct tgcagaactc gatgatctcc
     1261 tcggcgctca gctcgtagtc ctcggtcgac tccttcgcgt cgccgacctg gatgtcctcg
     1321 aagtactgca ttcagacgta cttctcgatg atcttcccga gacctggaac cgcactttcg
     1381 acgttctttc gggcggaact gcgcagcttt ccataggcgc ctttgaccga accgtgctct
     1441 gagctctggg ctcggctgtc ggtaacggcc agaagggctt cggccacctc cccggtgttc
     1501 cgggcaaagt actgactcac cgtctgacca ctggagacgg cttgtgcact aatcggttcg
     1561 agtgctgccg caaagtcggg aagcaaggcc ttcaccacgt tgcgcacgaa gccgggcttc
     1621 actccttgaa cgagcctgta cccggctttg accgcaacac cgctgatccc cttctgcttg
     1681 gcaacctctt cgtcgaccag gcgacacaca tcctcgatga tcgaagcgcg tttggcttca
     1741 tcactcaaca cgtcgttcag attcatggtc catccttttg ccctttgcag gccctgatgc
     1801 aagcgaagcc gccgcaatgc tcgattcgat cgcaacgact acggaatcga caatctcgcg
     1861 gcgctcgctc acgacgaaga gatactcctt gttccgaagg tggctcacgc ggccgacctt
     1921 ctctccactc gggttgtgga ttccaatctg cgcaccgacg tagcgtttga agtccacggc
     1981 tacgactccc acgtgccccc gcgtcgacag gagcgcggtc atctcctcac ggtcgacatg
     2041 cccttcgttg ttgaatgaaa cgagcatgtg cttgcatcgc accgcgtcga atagtgcctg
     2101 aaggccctgg cgaatgcgcc gcttcgagtt gaagtcgctc ttgtattcct tgcactgtat
     2161 ccgcttgttc gcgatcccgt acgtctcggg cttgtcccag cggaccagac tctcccagac
     2221 gtggtagttg cccatgtagc tatgctgatt gtaaggcgga tcgatgtacg cgatgtccgc
     2281 ttcgacctcg gcagccgcct cgacagcgtc gaggttcagc gcttgacccg gaccgggaag
     2341 gatctttgga acacgaagct cgatctcttt gaaggcgcga gcggcccact gcttcaagta
     2401 ggccatctga acgcccgtgg tggagtcgac tcgatcggcc gcctccatca aggacacgag
     2461 ggcgatcgct tcgagctcgg gttgcaagga ccgccggcgg atctcttcgc gaatcgcatc
     2521 gacacgcgcg ccgttccgcg gttggaaata ccgtgcgtct tcacaaaacg ctttagtgaa
     2581 gtagccggca tccggtgcta gctcggaaag ctcggcgatc aattgcgctg catcctcgag
     2641 ccagcggttc gcgtcggcct ggacgtagca gcgagccaag gtcgatgcgt agctcaggtg
     2701 atcgttcgcg atcacgtagc gcccagtcgc tttgaggcct cttgccacac gcgacgtgcc
     2761 ggaaaacaaa tcgagcaccc tgcccgactc cgggaacgcc gacacggcct tcaggatgtg
     2821 cggcaccagc agccgtttcg agcctatgta cttgatcatg aagtctgaga cgtcatcgcc
     2881 tggacgatgg cgtcccggag cgtagcagct tcatagggct tttgaacgaa acccaacgct
     2941 cccatgctgc gtagctcccg ctcgaggtcg cgatcccagt agccggagac gaagacgacc
     3001 ggcgcgtccg ggtcgatctc tttgagcttc ttgaaagcct ggtcgccggt cattccgggc
     3061 atgttcaggt ccatcaagac caaatcgggc cggggctgcg tctcggtata gatacgtagg
     3121 gcctcgtcgc cgctccgcgc gaaaatcacc cgatgtccgc tttgccgaac cacccgccca
     3181 gcacttcggc gcacgacgtc ttcgtcgtcg acgagcatga tcaaaagctg cgtcgcaggt
     3241 agcttgatcg ctggcaagct cggctcggtc gggatcatgg gacgctcgga ggtcagggga
     3301 aacaccacct gaaactcact gccgcgatcg ggcacgctgt gcaccgtgat gtggccgttg
     3361 tgagatttgg cgatctcgta cacggcggca agccccaacc cggcgcctac ctggtctccc
     3421 ttggtcgtga agaaaggatc gaacgcacgc tcccgcgttg catcgtccat cccaatcccc
     3481 gtgtcttcga cggtgatgat cgcatgagca accaaagggt tgaggggcgc tttctcgaga
     3541 tcgacgggat cggctttctt cgcgctgatc ttgagcttgc caccgttggg catcgcgtct
     3601 cgcgcattga tgagaaggtt catcagaagc tggtggagct gcccccggtc gccactcacc
     3661 ttgagcccgc ggtcgatgcg cgcgtcgatt cgaatgccgc ggtggaatgt gtgccgtgca
     3721 agctgcacga cgtggtggca cagctcgctg aggtccacct ccgtgtggct ccgctccact
     3781 tcacgggcga agccgagcag acggctggtc atttcggtcg cggtcgcagc agcagagcgg
     3841 atatcgttga acgcctcacg gacgtcggga tcactcagct tcgtttcacc aggaaggtcg
     3901 ttcaggaagt cgatactcga acgcacggca ccgagaagat tgttgaaatc gtgagcgacg
     3961 ccggttccga gtcgtccgat cgcttcgagc tttcgtctct gggcgacctg ctgttccatc
     4021 gggtcgtgat gggcgaacag aaggagcgag cttcctattt gaatgcggtc gccgtacgtg
     4081 agcacccgcc gcgtgaccgc catgccattg acctgcgttc cattgcggct ttcgagatct
     4141 tcgatgacgt aagagccatt ctcgtgctga tagatacggg cgtgaattct ggagacgagc
     4201 gtgtcgtcga gcttgacgct cgcgttgtca ccccgtccga gcgtgagctc tttgtcgatg
     4261 gcgtacttgt ccccagcgag cgtgccagcg agcacgacga tgcgcgcctg ctcctccttc
     4321 ttggagaggc tcttctcgga gaacacgtgg cggcgtgtga cctcggtggg atcgtgtcgg
     4381 gacatcagac ggtgggcact ccgggcccag ttagatccta gcctgc
//
LOCUS       DASRZF010000372         3496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2350928, whole genome shotgun sequence.
ACCESSION   DASRZF010000372 DASRZF010000000
VERSION     DASRZF010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2350928"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1308)
                     /locus_tag="VFG22_16470"
     CDS             complement(<1..1308)
                     /locus_tag="VFG22_16470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HET6417893.1"
                     /translation="MALVPLSRKGTRGASRPDTSAYLPTTRAEMDARGWHELDVLIVN
                     GDAYVDHPAFGGALIGRFLEGRGYRVGMIAQPKWDDPADVSRMGTPRLFVGITSGNLD
                     SMLNKLTAQKKVRSEDQYSPGGRTNMRPNRASIVYGNLCRQAFSGVPIVLGGIEASLR
                     RIAHYDYWSDSIRRSVLLDSKADMLIFGMGERPVWELADRLAAGESLEDVRNIRGTAH
                     VLRKGQWENIEPSRFIRDGNPVFLPSYEEIKASKERFSEMSGAFQKETNPGNGRPLIQ
                     PHEHEAVYLNPPAEPLSTELMDELYDLPFTRYPHPSYDAPIPAYETVKHSIVTMRGCF
                     GGCSFCSITEHEGRVIQSRSAESVLREVRALRRMDDFAGVISDVGGPTANMYQMKCKD
                     ERIEKACRRLSCVYPGVCENLVTDHAPLVELLGQVRKEPGIKKA"
     gene            1353..2642
                     /locus_tag="VFG22_16475"
     CDS             1353..2642
                     /locus_tag="VFG22_16475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417894.1"
                     /translation="MKGQEEPHARAGDRRSFEVESLLPSGEGVSGRTRIAGAFPGEQV
                     VARVEHVGKHASFATILEVERGRAGRRLPPCPRHVDLKDGSCTGCGLMALDESDQRTL
                     LREMLLAEHGLDVGEVVPARESLGYRWSAKRIAFGGPGKLRLGSFMRGTNRPADMKGC
                     LVDHPLIAAAADELAEAARDLGIAAHDEERKRLGLRAVWLRTNGTEVLATLVTSEANE
                     DELRRLSPRLTRCDGLAFSFHREGGNALRGAEATMLRGIEALDVNGTRIGPLGFLQPN
                     PRVSEQMYDDLVEGIGGGRILDLYAGSGATTRRLRAVASEVLACESHPEGASRLGIEA
                     ETAEAFLARQTEAPDAVVANPPRKGLGAAVTSQLLRLRPARIHVMACGPAGLAKDIAA
                     LADGYRLESLRAYDSLPQTPHLELIAKLVRIDEPASA"
     gene            complement(2665..3222)
                     /locus_tag="VFG22_16480"
     CDS             complement(2665..3222)
                     /locus_tag="VFG22_16480"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HET6417895.1"
                     /translation="MEATDMLMQEHRVIEKALDAMEGWSAKLGRATEPDEKAELARFV
                     AFIQGFADAYHHGKEEDILFVLMGQHGFPRHVGPIAVMLQEHDLGRSLVGVLGGLAKQ
                     SAPWSKEDRATIAQTVQEFSALLRQHIRKEDQVLYPMADARLPEPIKAEMFRRFQEFE
                     EQRTESGEQKRLRELGEALIQAHAP"
BASE COUNT          575 a         1036 c         1201 g          684 t
ORIGIN      
        1 ggccttcttg atgcccggtt cctttcggac ctggccgagg agctcaacga gcggggcgtg
       61 atcggtgacg aggttctcgc agacgcccgg atatacgcag ctcaggcgtc ggcaggcctt
      121 ttcgatgcgc tcgtccttgc atttcatctg gtacatgttc gcggtcggtc caccgacgtc
      181 gctgatcacg ccggcaaagt cgtccatgcg ccggagcgca cgcacttcgc ggaggacgct
      241 ctcggcgctt cgcgactgaa tcaccctgcc ctcgtgttcg gtgatcgagc agaagctgca
      301 gccgccgaaa cagccgcgca tggtcacgat gctgtgcttg accgtttcgt aggcaggaat
      361 gggcgcgtcg tacgaagggt gtgggtagcg cgtgaaaggg agatcgtaga gctcgtccat
      421 cagctccgtg ctgagcggct cggccggcgg gttgaggtag acggcctcgt gttcatgggg
      481 ctggatcaag ggccggccgt tgcccgggtt cgtttctttt tggaacgcgc cgctcatctc
      541 gctgaagcgt tccttgctgg ctttgatctc ctcgtagctc ggcaggaata cgggattgcc
      601 gtctcggatg aaacggctcg gctcgatgtt ctcccattgg cccttgcgca ggacgtgggc
      661 ggttccgcgg atgttgcgaa cgtcttcgag agactcaccg gcggcaagcc gatcggccag
      721 ctcccagacc gggcgttcgc ccatgccgaa gatcagcatg tcggctttgg agtcgaggag
      781 gacgctccgg cgaatcgaat cgctccagta gtcgtaatgc gcaatgcgac gaagggaggc
      841 ctcgatgcca ccgagcacga tcggcacgcc cgagaatgcc tgcctgcaaa ggttgccgta
      901 gacgatgctc gcgcgattgg ggcgcatgtt ggtgcggccg cctggcgagt attgatcctc
      961 ggagcgcacc ttcttctgcg cggtcagctt gttgagcatg ctgtcgagat tgccgctcgt
     1021 gatgccgacg aagagtcgcg gcgtgcccat gcgggatacg tcggcaggat cgtcccattt
     1081 gggctgggcg atcatgccga cacggtagcc acgcccttcg aggaagcggc cgatcagcgc
     1141 gccgccgaac gcggggtggt cgacgtaggc gtccccgttt acgatcagca cgtcgagctc
     1201 gtgccagccg cgtgcgtcca tttcggcccg ggtggtgggc aggtaggcgc tcgtatccgg
     1261 ccggctcgcg ccccgagtgc cttttcgcga taaggggacg agagccacgc cccaccttgc
     1321 ccatgagtga ccggtcgcgc aaacgtacac ccatgaaggg ccaagaagag ccgcacgcca
     1381 gagccggaga ccggcggagc ttcgaggtcg aatcgctgct gccttcgggg gaaggtgttt
     1441 cgggacgcac gcgcatcgcg ggtgcctttc ccggggagca ggtcgtcgcg cgtgtcgagc
     1501 acgtgggcaa gcacgcgagc tttgcgacga tcctagaagt ggaacgcggg cgcgcgggtc
     1561 ggcggctccc gccctgcccg cgtcatgtgg acctgaaaga cgggagctgc accggatgcg
     1621 gcttgatggc gctcgatgaa agtgatcagc ggacgctcct gcgggagatg ctgctcgccg
     1681 aacacgggct cgatgtgggt gaggtggtcc cggcgcggga atcgctcggc tatcgctgga
     1741 gtgcaaaacg cattgcgttt ggcggacctg ggaagctgcg cctcgggagc ttcatgcgcg
     1801 ggaccaatcg tcctgcggac atgaagggtt gcctggtgga ccatcctctg attgcggcgg
     1861 cggcggacga gctcgccgag gccgcacgtg acctcgggat cgcggcccat gacgaggaac
     1921 gcaagcgact cgggcttcgc gcggtctggt tgcgtaccaa tgggaccgag gtgctggcta
     1981 cgcttgtcac gagtgaagcc aacgaggacg agctacgccg gctcagcccg cggcttacgc
     2041 gctgcgatgg cctggcgttc agctttcatc gggagggcgg caatgcgctc cggggcgcag
     2101 aggcgacgat gcttcgcggg atcgaagcgc tcgacgtgaa cggtacgcgg atcggtccgc
     2161 tcggctttct gcaacccaac ccgagagtct cggagcagat gtacgacgat ctcgtggagg
     2221 gcattggtgg cgggcgcatc ttggatctct atgcaggttc gggcgcgact acgcgtcggc
     2281 tcagagccgt ggccagcgag gtcttggctt gcgagtcaca tcccgaaggc gcgagcaggc
     2341 ttgggatcga agcggaaact gcggaagcgt ttcttgcgcg gcagaccgag gcgccggacg
     2401 cggtggtcgc caatccacct cggaagggcc ttggcgctgc tgtgacgagc cagctgcttc
     2461 ggcttcgccc ggcgcgcatt cacgtgatgg cttgtggtcc ggctgggctg gcgaaggaca
     2521 tcgcagcgct tgccgacggg tatcggctgg aaagcctgcg cgcctacgac agcttgccgc
     2581 agaccccgca tctggagttg atcgcgaagc tggtccgaat cgacgaaccg gcatcggcgt
     2641 gaaccgcgtg cttggggcct cgcttcaggg agcgtgcgct tgaatcaagg cctcgccgag
     2701 ctcgcggagt cgtttttgtt cgccgctttc ggtccgttgc tcctcgaatt cttggaatcg
     2761 tcggaacatc tcggctttga tcggctccgg cagtcgggca tcggccattg ggtagagcac
     2821 ctggtcttct ttccggatgt gctggcgtag cagagcggag aactcctgca ccgtctgtgc
     2881 gatcgtggcc cggtcttcct tactccaggg cgccgattgt ttagcgagtc cgcctaggac
     2941 acccacgagg gaacggccga ggtcgtgctc ctgcagcatg acggcgatgg ggcccacgtg
     3001 gcggggaaag ccgtgctggc ccatcaggac gaagagaatg tcttcctcct tgccgtggtg
     3061 ataggcgtcc gcgaatcctt ggatgaacgc gacgaaacga gcgagctccg ctttctcgtc
     3121 tggctcggtg gctcggccca actttgcgct ccagccctcc atggcatcga gtgccttttc
     3181 aatgacgcgg tgctcttgca tcagcatgtc tgtggcttcc acttcggctc ccttcctcga
     3241 gttggcgagc tgcagcatcg cgaatggcgt ccggatggca acgctaccaa cccgacagtg
     3301 atctggcctc gtgaatcaca atgtgataca tgatttgcat gaatgtgtca cagcaatccg
     3361 ccgtagagca aaaagccctt acaaatcggg cacatgatga atcttcgtcc ttgcggtact
     3421 ggctggctgc ggtcgatgcc gtgctcaggg gcgtcgagga gaccgtctgg cgtctgcgtc
     3481 gcgaagcggt ggaggc
//
LOCUS       DASRZF010000373         9643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4415378, whole genome shotgun sequence.
ACCESSION   DASRZF010000373 DASRZF010000000
VERSION     DASRZF010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 9643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9643
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4415378"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VFG22_16485"
     CDS             <1..219
                     /locus_tag="VFG22_16485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417896.1"
                     /translation="EAYGTDAYYCDWLDTDESQALLNFQRISFEDFIGEMRRASRFTR
                     PLTRMFSPMIRWFMLRYSDAWQARKLDA"
     gene            complement(221..1003)
                     /locus_tag="VFG22_16490"
     CDS             complement(221..1003)
                     /locus_tag="VFG22_16490"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6417897.1"
                     /translation="MNKPPKYVEGHKLLEGKTVLVTAAAGTGIGFATAVRCAEEGASG
                     LMISDIHERRLGEAAEKIEQETGLRPDTVMCNVTNEDEVQAMFELAVEKMGHVDVLMN
                     NAGLGGYAKLVDMTDDQWNLVLDVTLNGCFRCTRAALKHMMPRGTGTIVNNASVLGWR
                     AQEGQSHYAAAKAGVMALTRCAAIEAAEHNVRINCVAPSLAMHPFLEKVTPPGLLEEL
                     KKREAFGRGAEPWEISNVMVFLGSEYSSYMTGEVLSVSSQRA"
     gene            complement(1000..2175)
                     /locus_tag="VFG22_16495"
     CDS             complement(1000..2175)
                     /locus_tag="VFG22_16495"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6417898.1"
                     /translation="MELQLSQEEESFRQEARAWLQEQLNGPFKDLRGRGASGDQDTYV
                     EERIAWGKVMGAAGWNCIGWPKAFGGRGCTIKEEAVFNEEYVRANGPGNVGHIGETLL
                     GPTLIHFGTQAQRERFLPPIVSGDEIWCQGYSEPGAGSDLANVQTKATLVGGEWVIEG
                     QKIWTSGAQYSDWCFVLCRTDSDAPKHRGISYILVPMKQDGIDVRPIKQMGGSAEFAE
                     VFFDGAKAASDHVVGGVNNGWKVAMGTLAFERGASTLGQQYLFQKEFEQIVEVAKRTG
                     ASKDPIFRQRIAAAWIGLKNMRVNALRTLEHEGADLSREALISKLYWSNWHRDLGKLA
                     MDLLGPEAELIEGPPYELTALQKWYLFSRADTIYAGSNQIQRNIIGERGLGLPKEPR"
     gene            complement(2175..3314)
                     /locus_tag="VFG22_16500"
     CDS             complement(2175..3314)
                     /locus_tag="VFG22_16500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6417899.1"
                     /translation="MEFSFSEAQDELRRAARRFLEVASSEERVRAAMETERGYDRGAW
                     TQLSEELAWTALTIPEELGGLGMSYLDLHPLMEEMGRVLLCAPYFSTICLGANALMLG
                     GSTEQQERHLPGIAAGELTATLAYSERNNRTDADGVETSYSWVGTECVLSGQKYYVLD
                     GHTADLLIVAARAKGSSGTEGVSLFLVPRDAAGMNCSWLPSMDQTRRLASVDLDGVRV
                     SSDALLGKEGSGWDLCERTLDLARIALAAEQVGAAEMCLDMSVEYAKVRKQFGRPIGS
                     FQAIKHKCADMLTMIESARSAAFYASALAAQGEEDLSEAASSAKAFCSDTFFHCAAEN
                     IQIHGGIGFTWEHPAHLYFKRAKASEVLFGAPSFHRERVAQRLEL"
     gene            complement(3319..4101)
                     /locus_tag="VFG22_16505"
     CDS             complement(3319..4101)
                     /locus_tag="VFG22_16505"
                     /inference="COORDINATES: protein motif:HMM:NF005893.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6417900.1"
                     /translation="MNEPLDFSGRAVLVTGGSRGIGKGIAEAFLAAGAKVAVCGRKKP
                     DVLPSMDGRTAVFVSADVRDPEQVEKMVEQVVAELGRIDVLVNNAGGAPFTDAATASP
                     RFSEKIIQLNLTAALHCAQQVNRVMQGQDSGGCIINIASVSGVRPSPGTAAYGAAKAG
                     LINLSATLAIEWAPKVRVNSIAAGLIRTEQAHLHYGNDEGIARVAETVPLKRLGTPED
                     VAGACLYFASPLAAYVSGANLLLHGGGETPAFLSAASVNRGD"
     gene            complement(4098..4976)
                     /locus_tag="VFG22_16510"
     CDS             complement(4098..4976)
                     /locus_tag="VFG22_16510"
                     /inference="COORDINATES: protein motif:HMM:NF005861.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6417901.1"
                     /translation="MIVTGAGRGIGREHALELARQGAQVVVNDLGVSNSGEGGGQGPA
                     DEVVDTIRAMGGKAVANGADIADWEQSEALVRQAIDEFGRLDAVVNNAGFVRDRMFVS
                     CSEEEWDAVIRVHLKGHFCVARHAAAYWRAEIKAGKEVDARIINTSSGAGVLGSVGQS
                     SYSAAKAGIATLTLVQAAELGRYGITANAICPIARTRMTEGAFGDTMKAPDQGFDEND
                     PANVSPFIAWLVSSQSREVTGRVWEVFGGTITVFDGYRREQTVDIGKRWDASEVGPAV
                     KGLLAKARPLVKVYGT"
     gene            complement(5000..6028)
                     /locus_tag="VFG22_16515"
     CDS             complement(5000..6028)
                     /locus_tag="VFG22_16515"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HET6417902.1"
                     /translation="MTRVGRQILLRRRPRGLPSTADFEARECEVASPGSGEVLLENLY
                     LSIDPAIRGWMSDAKSYLPPIGIGEPVRSGTLSRIVETRAEGWSVGQIVQALAAWESY
                     SLVPVGNLHGKVTPVEGIPLSAMLSVLGGTGLTAYFGLLEVGNPREGETVLVSAAAGG
                     VGSIVGQIAKIKGCRAVGLTGSDEKCAWLIDELGYDAAINYRASNLRAALKDACPNGV
                     DVFFDSVGGEILNTVLTRLNRHGRVVLCGAISQINESELPPGPKNYLQLLAKSARIEG
                     FTTLDFARRYDEARAQLAAWIRAGKIRYRDEIVEGLDAAPSHLLRLFNGAHRGKLMVK
                     LADAGVWE"
     gene            complement(6112..7014)
                     /locus_tag="VFG22_16520"
     CDS             complement(6112..7014)
                     /locus_tag="VFG22_16520"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HET6417903.1"
                     /translation="MNDTTIETDSGNFWRLTGACIAVLVPLNWTWYIWARGIPGSLTV
                     YIILGSALYFGLLRLVTGIRFSDISYEARFIVISSMLLLIFFLAFGRSDTYKHAIEPW
                     IPKGARSPLLGFFYFAASSVLLRFAIPYLLVRFKLEKAPRDYGYVVRGGTRYRWIYFG
                     LFVVMVPLVLWASTLPEFQASYPQTRGIVSGNEASVRLFVLYHAAYFLMFLSGESFWR
                     GYMTFGLGRDLDILALPWMVTLYSVGHYGKPILEVNGSIIAGLVLGYLALRHRSFFLG
                     ALLHWSIALTMDLAVLWQRGIVWN"
     gene            complement(7093..7632)
                     /locus_tag="VFG22_16525"
     CDS             complement(7093..7632)
                     /locus_tag="VFG22_16525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HET6417904.1"
                     /translation="MTELIGISGSLRKNSFNTALLTSASELVPEGTTLRVASLTSIPL
                     YNGDLDIDGGPEPIRALKEQIDAADGLVFATPEYNYGIPGVLKNAIDWVSRPAFRSVL
                     LGKPVAILGAAPGAVGTARAQAQLRQVLAGTLSEVFPHPEFLLAKAHERIQEGTLIDE
                     GSRVLLRDMLERYVSWIER"
     gene            complement(7637..8350)
                     /locus_tag="VFG22_16530"
     CDS             complement(7637..8350)
                     /locus_tag="VFG22_16530"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HET6417905.1"
                     /translation="MIRVRRSQDRGHAQHGWLDSRHSFSFGDYYDPAQMGVGPLRVIN
                     DDRVIPGEGFPTHPHRDMEIISYVVEGALEHKDSMGNGSLIRPGDVQRMRAGTGVTHS
                     EYNASASESVRFLQIWVIPSKRGLEPGYEQSFFGDERRGRLRLVASRDGAHGSVQIEQ
                     DVSMFASVLEKGQRVKHPLVTGRRAWLQVVRGALVVEGASLHEGDAALLTDVPWITID
                     SLDESELLLFDIASTEGEE"
     gene            8439..9323
                     /locus_tag="VFG22_16535"
     CDS             8439..9323
                     /locus_tag="VFG22_16535"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET6417906.1"
                     /translation="MDLNDLSIFVAVAEHGSFSKAASSLGLAKSTVSERVRALENELG
                     AQLLRRTTRRSNLTDAGRALLARGRTIVSLAAEAESELTAATQTAVGRLRMSAPISFG
                     LRFLTEIVAELARNQPQLAIDFQLEDRNVDLVAEGFDLALRIGRLPDSSMLAIGVGSS
                     RRLVVAAPAYVERHGAPTRPSELRDHECLLYTHQGEPDTWFFNRPDGSEERARVTGRL
                     KSNHGDALAELASEGAGLAWLPEFIVEPFIEQGRLVALLPDRCVAEVPIQVLFPPRSH
                     RTFKEQLLVDALKRRLAR"
     gene            9381..>9643
                     /locus_tag="VFG22_16540"
     CDS             9381..>9643
                     /locus_tag="VFG22_16540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417907.1"
                     /translation="MHRLKPAFLAISMSFATLPSTAIAEPSARAPSPEEADSSPGTAL
                     PAKYQATLAKPPNVEWSLTAELGFLSVVSHIVQFGQDGSRFNYR"
BASE COUNT         1843 a         3096 c         2789 g         1915 t
ORIGIN      
        1 gaggcatatg gtaccgacgc gtactactgc gattggctag acaccgacga aagtcaggcg
       61 cttctaaatt ttcaacgaat cagcttcgag gatttcatag gcgagatgcg ccgggcctcg
      121 cggttcaccc ggcccctgac acggatgttt tccccgatga tccgctggtt catgttgcgg
      181 tactccgatg cctggcaggc tcggaagcta gatgcctagc tcaggcgcgc tggctcgata
      241 ctgaaagcac ttcgcccgtc atgtacgagc tgtactcgct gccgaggaag accatgacgt
      301 tcgagatttc ccaaggctct gcgccgcgac caaaagcctc gcgctttttc agctcctcga
      361 gcagtcccgg tggtgtgact ttctccaaaa aagggtgcat cgccagcgaa ggtgcgacgc
      421 aattgatgcg cacgttgtgc tcggcagcct cgattgcggc gcagcgggtc aatgccatta
      481 cgcctgcttt ggcagctgcg tagtgcgatt gcccttcttg cgcccgccat ccgagcaccg
      541 aggcgttgtt cacgatcgtc ccggtgccac gcggcatcat gtgcttcagt gcagcgcgcg
      601 tacaacggaa acaaccgttg agcgtgacgt cgagaacgag attccactga tcgtcggtca
      661 tgtcgaccag cttcgcgtat ccacccaagc ctgcgttgtt catcaacacg tcgacgtgcc
      721 ccatcttctc gactgcgagc tcgaacatgg cctgcacttc gtcttcgttg gtgacgttgc
      781 acatcacggt atctgggcgc aggcccgttt cttgttcgat cttctctgct gcttcgccca
      841 gacggcgttc gtgaatgtcg ctgatcatca ggcccgaggc gccttcttcg gcgcaccgaa
      901 cggcggttgc aaatccgatg ccggtgcccg cggccgccgt gacgagcacg gtcttgcctt
      961 cgagaagctt gtgcccttcg acgtacttcg gtggcttgtt catcgcggct cctttggcag
     1021 tccgagtcct cgttctccga tgatgtttcg ttgaatttgg ttcgacccag cgtagatcgt
     1081 gtcagccctc gagaagagat accacttctg caaggcagtc agctcgtatg gcggaccttc
     1141 gatcagctcg gcctccggcc cgagcaggtc catggcgagc ttcccgaggt cacgatgcca
     1201 gttcgaccaa tagagcttgc tgatcagcgc ctcgcgactg aggtccgcac cctcgtgctc
     1261 gagtgtacga agtgcgttga cacgcatgtt cttgagcccg atccaggcgg cggcgatacg
     1321 ctgcctgaaa atcgggtctt tcgatgcgcc cgtacgtttt gcgacttcga cgatctgctc
     1381 gaattccttt tggaatagat actgctggcc gagcgtcgac gcgccgcgct cgaaggcgag
     1441 cgtgcccatg gcgactttcc aaccgttgtt cacgccaccc acgacgtggt ccgaagccgc
     1501 cttggcgccg tcgaagaaga cctctgcgaa ctccgcgctt ccacccatct gcttgatggg
     1561 gcgcacgtcg atgccgtctt gcttcatggg aaccaggatg taactgatgc cccgatgctt
     1621 cggcgcatcg gagtcggttc ggcaaaggac gaagcaccag tccgagtact gagcgcctga
     1681 agtccatatc ttctggcctt cgatgaccca ctcgccaccc accagggttg ccttggtctg
     1741 cacgttcgcc aggtccgacc ccgctccggg ctcggagtat ccctgacacc aaatctcgtc
     1801 cccagaaacg atgggaggca aaaagcgctc tctctgagcc tgcgtaccaa aatgaatcag
     1861 cgtgggccca agcagcgttt cgccaatgtg tccgacgttg cccgggccgt tcgcccggac
     1921 gtattcctcg ttgaatacgg cttcttcttt gatcgtgcag cctcggccgc cgaacgcctt
     1981 cggccagccg atgcagttcc aaccggccgc ccccatcacc ttgccccagg cgattcgttc
     2041 ctcgacgtac gtatcctgat cgcctgaagc cccgcggcct cgaagatctt tgaaggggcc
     2101 gttgagctgc tcttgcaacc aggcccgtgc ctcctggcgg aagctctcct cttcttggct
     2161 gagttgaagc tccatcacaa ctccaagcgt tgcgcgacgc gttcgcggtg aaaagaaggg
     2221 gcgccgaata ggacttcgga agccttggcc cgtttgaagt acaggtgcgc cgggtgctcc
     2281 caggtgaaac caatcccccc gtgaatctgg atgttttctg cggcgcaatg gaagaacgta
     2341 tctgaacaga acgccttcgc actggaggcc gcttccgata gatcttcttc cccctgggct
     2401 gcgagcgcac ttgcgtagaa tgccgccgaa cgcgccgatt cgatcatcgt gagcatgtcg
     2461 gcgcatttgt gcttgatggc ttggaacgaa cctatgggcc tgccgaactg cttgcgaacc
     2521 tttgcgtact cgaccgacat atccaggcac atctctgcag cgccgacttg ctcggccgcc
     2581 agagcaatcc gggccaggtc cagagttcgc tcacaaaggt cccagcccga gccttccttc
     2641 ccgaggagcg catcggagga gacgcgcacg ccatcgagat ctaccgacgc caagcgccgc
     2701 gtttgatcca tgctggggag ccatgagcaa ttcatgcccg ccgcgtctct cggcaccaag
     2761 aagaggctca cgccttctgt tccactgctg cctttcgcgc gagcggcaac gatgagaaga
     2821 tcggccgtgt gtccatcgag aacgtagtac ttctggccgc tcagaacgca ttccgttcca
     2881 acccatgagt agcttgtttc gactccgtca gcgtccgtgc ggttgttccg ctcgctgtac
     2941 gcaagcgtcg cggtgagctc gcctgcggcg attccgggca gatggcgctc ttgctgctcg
     3001 gtagatccgc cgagcatcaa cgcgttcgcg ccgaggcaaa tcgtcgagaa gtagggtgcg
     3061 cagagcaata cgcgacccat ctcctccatc aagggatgaa ggtcgagata actcatgccg
     3121 aggccgccga gctcttcagg aatggtgagc gcagtccatg ccaactcctc ggaaagctgc
     3181 gtccaggcgc cacggtcgta gcccctctcg gtttccatgg cggcgcgcac acgctcttcc
     3241 gaagaggcga cctccaagaa ccggcgcgcg gctcgtctaa gctcatcctg cgcttctgag
     3301 aatgaaaatt ccattcactc aatctcctcg attgacgctg gcggccgaga gaaacgcggg
     3361 tgtttctccc cccccatgaa gaagcaaatt tgcgccgctc acgtatgcgg caagaggcga
     3421 ggcgaagtac aggcacgcgc ctgcgacgtc ttcgggagtg ccaagtcgct tcaacggaac
     3481 ggtctcggcg acacgtgcga tcccctcgtc gttgccgtag tggaggtgcg cctgttcggt
     3541 tcgaatcaat ccggctgcga tcgagttcac tcgaaccttg ggagcccatt cgattgcgag
     3601 tgtggcactg agattgatca atcccgcctt tgccgcgccg tatgctgccg tcccgggaga
     3661 ggggcgcacg ccggagacgc tggcgatgtt gatgatgcaa ccgccagaat cctgcccttg
     3721 catgactcga ttgacttgct gagcgcagtg cagcgccgcg gttagattca attgaatgat
     3781 cttctctgaa aaccgtggtg atgcagtggc cgcatcggtg aaaggcgcgc cgcccgcgtt
     3841 gttgaccaag acgtcgatgc gtccgagctc tgcgaccacc tgctcgacca tcttctccac
     3901 ctgctcgggg tcgcgaacgt ccgcactcac gaagacggcg gtcctgccat ccatgctggg
     3961 gagcacatcg ggtttcttcc ggccacagac cgccaccttc gcgcccgccg ctaggaaagc
     4021 ctccgcgatc cctttgccga tcccgcggct tcctccggtt accaggaccg cgcggccgct
     4081 gaagtcgaga ggctcgttca tgtgccgtac accttcacca aggggcgcgc tttggcgagg
     4141 aggcccttca cggcgggccc aacctcagaa gcgtcccagc gcttgccgat gtcgaccgtc
     4201 tgttcgcggc gatacccgtc gaaaaccgtg atggttccgc caaaaacctc ccagacacgt
     4261 ccggtgactt cgcgcgattg cgagctgacc agccacgcga tgaaaggtga cacgttggct
     4321 gggtcgttct cgtcgaaacc ctggtctggc gccttcatcg tgtcgccgaa ggcaccttcg
     4381 gtcatccgcg tccgtgcgat cggacaaatc gcgtttgcgg tgattccgta tcgtccgagc
     4441 tcggcggcct gcactagcgt caaagttgca atccctgctt ttgcggcgga atagctcgat
     4501 tgaccgacac tgcccagcac tcccgcgccc gagctcgtgt tgatgatccg tgcatcgact
     4561 tcctttcccg ctttgatctc agctcgccaa tatgcggccg cgtgccgcgc aacgcagaag
     4621 tggcccttca aatgcacccg gatgaccgcg tcccactcct cttcgctgca actcacgaac
     4681 atgcggtccc gcacgaagcc cgcgttgttg acgacggcat cgaggcgccc gaactcgtcg
     4741 atggcctgtc ggaccagggc ctcggactgt tcccagtccg cgatgtccgc accgttcgcc
     4801 accgctttgc cacccatggc gcggatggta tcgacgacct cgtccgcagg gccttggccg
     4861 ccgccttcgc cactgttgct cacgcctagg tcattgacga cgacctgcgc cccttgcctc
     4921 gcgagctcga gcgcgtgctc ccgtccgatg ccgcggcctg cgccggttac aatcacagtc
     4981 cgtccatcac atatgccagt cattcccaaa cccctgcgtc ggccaacttg accatcagtt
     5041 ttcctcgatg cgcaccgttg aagagtctca gcaagtgcga cggagccgcg tcgagaccct
     5101 cgacgatctc gtcgcgatag cggatcttgc ccgcgcgaat ccaagctgcg agctgggcgc
     5161 gcgcttcgtc gtagcgtcgc gcgaaatcca aagtggtgaa gccctctatt cgagcgctct
     5221 tggcgagaag ctgcaggtag ttctttggac cgggaggaag ctcggactcg ttgatctggg
     5281 aaattgcgcc gcagagcacg acgcggccgt ggcggttgag tcgtgtgagc accgtgttga
     5341 ggatctcacc gccgacgctg tcgaagaaca cgtcgacacc attcggacat gcgtccttga
     5401 gcgcggcccg cagattcgaa gctcggtagt tgatcgctgc gtcgtagccg agctcgtcga
     5461 tcagccacgc acatttctca tcgctgcccg tcagcccgac ggcccgacaa cctttgatct
     5521 tcgcgatctg cccgacgatc gaacccacgc cacccgcagc cgcagacacg agaacggttt
     5581 ccccctcgcg cggattgccg acttccaaga gcccaaaata agcggtcagt ccagtgccgc
     5641 caagcacgct caacatcgcc gagagcggga tgccttccac cggcgtcacc ttgccgtgaa
     5701 gattgcccac cgggaccagg ctgtacgact cccaggccgc cagagcctgc acaatctgcc
     5761 caacggacca gccctcggca cgcgtctcga cgatccgtga aagagttccg ctccgaacgg
     5821 gctcgccgat cccgatcgga ggcaagtacg acttggcgtc gctcatccac ccgcgaatcg
     5881 ccggatcgat cgacagatag aggttctcga gcagcacctc tccggaacca gggctcgcca
     5941 cctcgcattc ccgggcttcg aaatccgctg tggaaggcag ccctcttggg cgtcttctca
     6001 acagaatctg ccgtccaacc cgggtcacag gtccaagact gaaacatgtt tcactttctg
     6061 caaggggacc cgcgcgcgag ctcggtttac gtcggcccga ggggggggtc cttagttcca
     6121 gacgatccct ctctgccaga ggaccgcgag gtccatcgtg agcgcgatcg accagtggag
     6181 gagggcaccg aggaagaacg agcggtgtcg aagcgcgagg tagcctagaa cgagcccggc
     6241 gatgatcgag ccgttgacct cgaggatggg tttcccgtag tggccgacac tataaagcgt
     6301 gaccatccaa ggcagcgcca agatgtccag gtcacgaccg aggccgaagg tcatgtagcc
     6361 ccgccagaag ctctccccgg ataggaacat caggaagtaa gccgcgtggt agagaacgaa
     6421 aagccggacc gaggcttcgt tgccgctgac gatccctcgc gtctgcggat agctcgcttg
     6481 gaactccggc agcgtcgagg cccaaaggac cagcggaacc atgacgacga acagcccgaa
     6541 atagatccat cgatagcgtg ttccgccccg aacgacatat ccataatcgc gaggggcctt
     6601 ctcgagcttg aagcgaacga gcaggtacgg gattgcgaag cggagcagca cactggaggc
     6661 cgcgaagtag aagaaaccaa gcaaaggtga gcgcgcgccc ttcggaatcc acggctcgat
     6721 cgcatgcttg taagtatccg agcgaccgaa cgccaggaag aagatcaaga gcagcatcga
     6781 gctgatgacg atgaatcgag cctcgtagga aatgtccgaa aagcgaatgc ccgtgacgag
     6841 gcgcagcagt ccgaagtaga gcgcgcttcc gaggatgata tagaccgtca acgagcctgg
     6901 aatcccgcga gcccagatgt accaagtcca attcaagggc acgagcacgg cgatgcaggc
     6961 tcccgtcaat cgccagaaat tcccgctatc ggtttcgatc gtagtgtcgt tcaagcgcgc
     7021 cgcctcaacc ggctttagcc ctttggcccg acttgatcaa aaagggcgta tcggagatcg
     7081 ctaggtcggc agctagcgct cgatccaact cacgtatcgc tcgagcatgt ctctcaggag
     7141 gacgcgagag ccctcatcga tgagagtgcc ttcttggatt cgctcgtgcg ccttggccaa
     7201 gaggaattct ggatgtggga acacctccga cagcgtcccc gcgaggacct gcctcagctg
     7261 cgcctgcgcc ctggccgtgc cgaccgcgcc cggcgcggct ccgaggatgg cgactggctt
     7321 gccgaggagc accgacctga acgccggccg cgaaacccag tcgatcgcgt tcttgaggac
     7381 cccgggaatg ccgtaattat actccggcgt cgcaaacacc aggccgtccg ccgcgtcgat
     7441 ctgctccttc aacgcccgaa tcggctcagg accgccatcg atgtccaggt ctccattgta
     7501 aagaggaatg gaggtgagcg aggcgactcg aagcgtcgtt ccctccggca ccaactccga
     7561 cgccgacgtc aggagtgccg tgttgaatga gtttttccgt aggcttcctg agattccaat
     7621 gagctcagtc atggtgttac tcctcgcctt ccgttgaggc gatgtcgaat agaagcaact
     7681 cgctctcatc gagcgagtcg atcgtgatcc acgggacatc ggtcagcaac gcagcgtctc
     7741 cttcatggag cgaagcgcct tcgacgacaa gggcgccgcg aacgacttgt agccaagctc
     7801 gtcggcccgt cacgagtggg tgcttcacac gctgcccctt ttcgagaacc gatgcgaaca
     7861 tcgacacgtc ctgctcgatc tgaaccgaac cgtgggcgcc atcgcgggac gcaacgagtc
     7921 gaagccggcc gcgacgttca tctccgaaga agctctgctc gtagccgggt tccagcccac
     7981 gcttcgaggg aatcacccaa atctgaagaa atcgcaccga ctcgctcgcc gaggcgttgt
     8041 actcgctatg cgtcactccc gttcccgcac gcatccgctg aacgtctcca ggacgaatca
     8101 gcgagccgtt acccatactg tccttgtgct cgagcgcccc ttcgaccacg taggaaatga
     8161 tttccatgtc gcgatgtgga tgcgttggga acccttcgcc gggaatcacg cggtcatcgt
     8221 tgatcacgcg cagcggtccg actcccattt gggccgggtc gtagtagtcg ccaaacgaga
     8281 agctatgtcg actgtcgagc cagccatgct gagcatggcc ccggtcttgg ctacggcgaa
     8341 ctctgatcat ggctgcatcc tccttcccga aaacctaagg agtttgaaaa gacaaacaat
     8401 atgacataca gtgacatcac tgttcggaaa tccgcacaat ggatctcaac gatctctcga
     8461 tattcgtggc cgtggcagag catggaagct tctcgaaggc ggcttcgtcg ctcgggcttg
     8521 cgaagtcgac ggtcagcgag cgagtccgcg ctctcgaaaa cgaattgggc gcccagcttt
     8581 tgcgtcgcac cacccgacgt tcgaatctga ccgatgcggg tagggcgctc ctcgctcgcg
     8641 ggcggaccat cgtctcactg gcggccgagg ccgagagcga gctgaccgct gctacgcaaa
     8701 cggcggtggg ccgacttcga atgagcgcac cgatctcgtt cgggctgcgg tttctgaccg
     8761 aaatcgttgc cgagctcgca cgaaaccagc cccagctcgc aatcgatttc cagctcgaag
     8821 atcgcaacgt cgatctggtt gcagagggct tcgacctcgc gctgcggatc ggtcggcttc
     8881 cggactcatc gatgttggcg atcggtgtcg gttcctctcg gcgtctcgtc gtggctgctc
     8941 ctgcctacgt cgagcgccat ggtgcgccga cgagaccctc cgagcttcga gaccatgaat
     9001 gcctcctcta tacgcatcag ggtgaaccgg atacttggtt cttcaatcga cccgatggat
     9061 cggaagagcg agctcgggtg acgggtcggc tcaagagcaa ccacggcgac gcactggctg
     9121 agcttgcatc ggaaggagcc gggcttgcct ggcttcccga gttcatcgtg gagcccttca
     9181 tcgagcaggg acggctcgtt gcgttgctcc ccgatcgctg cgttgcggag gtgcccattc
     9241 aggtgctgtt tccgcctcgc tcacatcgga ccttcaagga gcaactcctg gtcgacgcgt
     9301 tgaaacgtcg cctcgctcgc tgagcattgc cgtccttcct ctgcatctcc gaatgccggt
     9361 gtgataccct gcggggcagc atgcatcgac tcaagccggc atttctcgcc atctcgatgt
     9421 ccttcgcgac cttgccgtcg accgcgattg cagagccatc ggcccgagcg ccctctccgg
     9481 aggaggcgga ttcaagcccg gggactgctc tcccagccaa gtatcaggcg acgctggcaa
     9541 agccgcccaa cgtagaatgg tcgctcaccg cggagctcgg cttcctgtcg gtcgtgagtc
     9601 acatcgtcca gttcggccag gacgggagcc gtttcaacta ccg
//
LOCUS       DASRZF010000374         7543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_5096751, whole genome shotgun sequence.
ACCESSION   DASRZF010000374 DASRZF010000000
VERSION     DASRZF010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 7543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7543
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5096751"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="VFG22_16545"
     CDS             complement(<1..580)
                     /locus_tag="VFG22_16545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417908.1"
                     /translation="MSGRAKEVALDQLVRVLDQVPLVSSIKNDLAGLRSLLLRRRPPR
                     IAALGLASSGRSTLLRALIERRPTRDALGAEHGQWVHLEHEGAKLHWLEIDVDDEAAR
                     SQWKAALDRELPDLVFLTFEPRNAGDLERIVDRAKSLLPDLPEGTAALRVFPILTHSD
                     LIGDGPRDVETARKELEAKLQDRGLLADPPRAV"
     gene            648..1805
                     /locus_tag="VFG22_16550"
     CDS             648..1805
                     /locus_tag="VFG22_16550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417909.1"
                     /translation="MLEADWLDSLEDLGGRLVPAAKNFVDSQRPPLPGTGAKGIRWLA
                     EQLEDFVDRETDAPEDDRFVEGAGALLGLLLIDHLGGRAREREGSHRVQLGRFGWFDP
                     FGAIQEILDAEDPRACFAEYLSMAEREADANGPVSGTVRVFADALGRARPDLDIESQF
                     ELTVELNNGASVDLTRLERIAREVGDGDAATEAARRIISMLPGSNAPEDTDWQEAAPR
                     LLPRLVSEKFVASLPSEQDLYLRPIGGDVHLSLQLRYGARARYVRRAEVDAWALAETD
                     ARGRAIENLVSKSQQLRIEPVEEGILRVRQGDGLDGARLILPDLQARLAKFGPGTWLA
                     AAPHRDVLLLAGETAGEALSRRAQDAARRAPHPVSPAVFVITSEGPRLLRT"
     gene            complement(1802..2875)
                     /locus_tag="VFG22_16555"
     CDS             complement(1802..2875)
                     /locus_tag="VFG22_16555"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6417910.1"
                     /translation="MASKPQPNLSTRLRRAPTRYEPTKRIAVGGMAEVWHANAIFDDG
                     GLHPVAIKRVIPSIGDPMFRSMFLDEARLGMLLRHPNIVRVYDARDIDGTYIMVMELV
                     DGQSLRSLTRVAQAENRSMGIPLAAWIAQEVAKALAYAHRARDAAGRGLGIVHRDVSP
                     QNILLGVNGEVKLADFGLADATVHEHVRAEDMIGGKIAYLAPEVLRGEGGDHRTDLFA
                     LGVVFWEMLAGRQLFSGDNDAHTIRQIAKCEVPPLADLVSGGDEADMLIRRLVTRNPD
                     DRIHDAAILASQLRHWIKGKQWQVGREDVANAVRTYRAERSRMSQAPPVSTLLADELE
                     KFAEATADTPFDLGASPLDPASF"
     gene            2937..5714
                     /locus_tag="VFG22_16560"
     CDS             2937..5714
                     /locus_tag="VFG22_16560"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HET6417911.1"
                     /translation="MRDHPYAPFIDRVHKPAQYLGGEQGEAHKDWNEVDCRICLAFPD
                     LYEIGMSHLGYKILYDIVNRNPAFLAERAYAVWDDMERELRSSEQSLCSLESARPLRD
                     FDIVGFSLQYELTYTNVLQILDLGKIPLRSEDRTNADPLIVAGGPTATHAEPMAEFVD
                     AFLIGDGEAKLPELMKSWAEMRDQGLPRAERLLRVAKLGGFYVPALYQSVPVEETRMQ
                     VVEPIHPEAPFPVRRSIVPDLNEYPFPVDGPIANCQTVFDRASIEVARGCTEGCRFCQ
                     AGMIYRPVREREPDAIIDAMLRSVAEAGYDEASLTSLSTADYSAIAPLVHQSIDALAP
                     YQVSLSVSSLRAYGLSEDVLDDMKRQRAGGLTFAPEAGSQRMRDVVNKNVTDAQLMET
                     AERVFSRGWSKMKLYFMIGLPTEEDEDVRDIVRTGARALEVARRVQRNKRARVTVSVS
                     THVPKPHTPFQWCAMDSQDEIKRKQSVLRNEASSFRIQLKMHNSDGSWLEGMLARGDR
                     RLADVIQGAYVRGARFDSWDERLDLAHWTDSIEDAGIDPAWFLGTIPVSAQLPWSHID
                     VGLEQGFLAREYRKSLRNRLSPPCGKTVGSFVHHTNVKEANDEKRRLVCYDCGIACDL
                     SEMKDERIRFLESMNAFEPRKTPAPDEPADEDNVVDRRPFYGVDQGAPMRIRIGYRKI
                     GRTAYTSHLDLVRAFPRMLRRVGLPLFYSEGFRPLPKLTFGPALPVGTASLCEHVDVR
                     LRADEHPDLNRLCARLNDAAIDGIEFFEHRVLGPRDAALNRVIEEADYVAAFSRQELR
                     SLGIEDEDTLARMLSERPSELFIDREVQGVKKRIDVGAALVDLRVGEGRNALALAGLA
                     GDLLPIRLTLRIGGKTTPRPGEALRALTGLDELVPRVVRCGLFANRPTGRVAPLQLEL
                     LRVKPTLQAAE"
     gene            5711..6325
                     /locus_tag="VFG22_16565"
     CDS             5711..6325
                     /locus_tag="VFG22_16565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417912.1"
                     /translation="MTDRFDIVRSLVRAAADRAVDRLASTRVAAATKQLVERLTKQRW
                     RLSDETLTAAAAHAHGVDSAMVACHSGRLFVDASMTNGREVQFSLTPFSVRFAPRGAK
                     EISFDVDPPDAHDSSIVGALAGCIAKATWPMIASLDAVDMGGAIVERESPGRLRVDLR
                     SVPAVRKFASSQTATMIFDVLELESIRVEPGGLALKLKLPQLTP"
     gene            complement(6322..>7543)
                     /locus_tag="VFG22_16570"
     CDS             complement(6322..>7543)
                     /locus_tag="VFG22_16570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HET6417913.1"
                     /translation="LQGAPVYYDCSTDDARLTLETVIDAIRNGGVAVNWVRVDALTKN
                     EQGRVSGVVVKNVRDGSLREVKAHTVINATGPWTDEVLAMSGARKGKVLRPTKGIHIV
                     VDREKLPVEHAVVLFHPTDKRVLFALPWGERTYVGTTDTDYDGAPGEEAATLEDVDYL
                     IAAANYYFPTKQISRDDVISTWAGLRPLIAPEPEVGEMAESQVSREHQIMIGEDGLIT
                     IAGGKLTTYRKMAKECVDVAVNLLKLSGKLPEDIHSGQTFKFPLPGAVGWPEDDDHQK
                     VADEIGRLCKCDLSDKARLHLVDTYGMRALELAKLCAADPSLIEAIIPGRVEIMAQVD
                     FGVLEELAASLSDIMIRRTQIFFRDSRQGLGSAEKVASRMAELIGWSDEEKQKQIDDY
                     KAQVALSQRWREEL"
BASE COUNT         1382 a         2437 c         2320 g         1404 t
ORIGIN      
        1 acacggcacg cggtgggtcc gccaggagtc cgcgatcctg aagcttcgcc tcgagctctt
       61 tgcgggccgt ctccacatcg cgcggcccat ccccgatcaa gtcggagtga gtgagtatgg
      121 ggaacacgcg cagcgctgcc gtcccctccg gaagatcggg cagcaggctc ttggctcggt
      181 cgacaattcg ctcgaggtcg cctgcattcc tgggctcgaa ggtgaggaac acgagatccg
      241 gcaactctcg atcgagcgct gccttccact gtgaacgcgc cgcttcgtca tcgacgtcga
      301 tctccagcca gtgcagcttc gcgccttcat gctcgaggtg aacccattga ccatgctcgg
      361 cgccgagcgc gtccctcgtc ggccgccgct cgatcagtgc gcgcagcagc gtcgagcggc
      421 cgctgctggc aaggccgagg gcggcaatgc gcggcgggcg gcgacgcagg agcagtgaac
      481 gaagcccggc cagatcgttc ttgatactcg ataccagggg tacctgatcg agcactctca
      541 cgagctggtc caatgccact tcttttgcgc gcccgctcac cggccaattc attcgtccta
      601 cggtctaggc gtgcaacccg agtcatgtta acttcgccgc cgcgtagatg ctcgaagcgg
      661 actggctcga ttctcttgaa gacctgggtg gccgtctcgt gccggcggcg aagaatttcg
      721 tggactccca gcgccctccg ctccccggaa cgggagccaa ggggattcgc tggctcgcag
      781 agcaactgga ggatttcgtc gatcgcgaga ccgacgcgcc ggaggacgat cgcttcgtcg
      841 agggcgcggg ggcgcttctc ggtctcttgc tgatcgacca cctgggcgga agggcgcgcg
      901 aacgcgaagg cagtcatcga gtccagctcg gacgctttgg ctggttcgat cccttcggtg
      961 ccatccagga gatcttggac gcggaggacc cgcgcgcgtg cttcgcggag tacctcagca
     1021 tggcagagcg cgaggcggat gcgaacggac ccgtttccgg caccgtgcgg gtgtttgccg
     1081 atgcgctcgg ccgcgcccgg cccgatctcg acatcgagtc ccagttcgag ctcacggtcg
     1141 agctcaacaa cggagcgagc gtagacctca cgcgtctcga acggattgcg agggaagtcg
     1201 gcgacgggga cgcggctacc gaagccgccc gcagaatcat cagcatgttg cccggttcga
     1261 atgctcctga ggataccgat tggcaggaag cggctccgcg tctccttccg cgtctcgtct
     1321 cggagaagtt cgtcgcgtca ctgcctagcg aacaggacct ctacttgcgt ccaatcggcg
     1381 gtgacgtgca cctctcgctt cagcttcggt acggagctcg tgcccgctac gtgcgccgtg
     1441 ccgaagtcga cgcctgggcg ctcgcagaaa ccgatgctcg cggacgcgcg atcgagaacc
     1501 tggtctcgaa gtcgcagcag ctacgaatcg aacccgtcga agagggcatt ctacgcgtcc
     1561 ggcagggtga cggattggat ggcgcgcggc tgattttgcc ggaccttcaa gcgcgcctcg
     1621 ccaagttcgg cccaggcacc tggctagcag ctgcccccca tcgcgacgtg cttcttctcg
     1681 ccggcgagac ggcgggcgaa gcgctcagca gacgagccca ggacgccgcg cgccgtgcgc
     1741 cacatccagt gtcgcctgcg gtcttcgtca tcacttcgga aggcccacgc ctgcttcgaa
     1801 cctagaagct ggctggatcg agcggggacg cgcccaagtc gaatggcgtg tcggccgtgg
     1861 cctccgcgaa tttttcgagc tcatctgcga gaagcgtgga gaccggcggc gcttggctca
     1921 tgcggctccg ctccgctcga taggtccgaa ccgcattcgc cacgtcttcg cggccgacct
     1981 gccactgctt ccccttgatc cagtgccgaa gctgcgacgc gagaattgct gcgtcatgga
     2041 ttcggtcgtc cggattgcgc gtgacgagtc gtcgaatgag catgtccgcc tcgtcgcctc
     2101 ccgaaaccaa gtcggccagc ggtggcactt cacactttgc gatctgacgg atggtgtgcg
     2161 cgtcgttgtc cccggagaac aattggcgtc cggccagcat ctcccaaaag acgacgccta
     2221 gggcgaacag gtccgtgcgg tggtcgcctc cctcgccgcg caacacctcc ggggcgaggt
     2281 aggcgatttt gcctccgatc atgtcctcgg cgcgcacgtg ctcgtggacg gtcgcgtccg
     2341 ccaatccaaa gtctgcgagc ttaacttccc cattgacacc gagaagaatg ttctgcggcg
     2401 atacgtcgcg gtgcacgata ccgagtccac ggcctgccgc atcgcgcgca cgatgggcgt
     2461 acgcgagcgc cttggcgacc tcttgcgcga tccaggctgc cagcggaatc cccatcgaac
     2521 gattctcagc ctgggccacc cgtgtcagcg agcgcagaga ctgcccgtcg acgagctcca
     2581 tcaccatgat gtacgttcca tcgatatcac gcgcgtcgta gacgcgaacg atgttggggt
     2641 ggcgcaagag catcccgagc cgcgcctcgt cgaggaacat cgagcgaaac atcgggtcac
     2701 caattgaggg gatcacccgc ttgatcgcaa ccgggtgcag gccgccgtcg tcgaagattg
     2761 cgtttgcgtg ccagacctcg gccatgccgc cgaccgcaat tcgcttggtc ggttcgtatc
     2821 gcgtgggcgc gcgtcgcagc cgagtcgaga gattgggttg cggcttcgag gccatgatgc
     2881 gagcgtggca aactgctcgg gacccgtcaa actagtccat cattcgagcc ttcatcatgc
     2941 gagaccatcc ctacgcgccc tttatcgacc gagtgcacaa accggctcaa tatctaggcg
     3001 gcgaacaggg ggaggcgcac aaagactgga acgaggtcga ctgccgtatc tgcttggcct
     3061 tcccggatct gtacgagatc ggaatgagcc acctcggcta caaaattctc tacgacatcg
     3121 taaatcggaa ccccgcgttt ctggccgagc gcgcctacgc cgtctgggac gatatggagc
     3181 gcgagcttcg ctctagtgaa caatcgctct gctcactcga aagcgcacgg ccgctccgcg
     3241 atttcgacat cgttggcttc tcgcttcagt acgagctgac ctacacgaac gttctccaga
     3301 ttctcgacct cggaaagatc ccgcttcgaa gcgaagacag aacgaatgcc gatcccttga
     3361 tcgtagccgg ggggccgacg gccacccacg cagagcccat ggcggagttc gtcgatgcgt
     3421 tcctgatcgg agacggcgaa gcgaaactgc ctgagctcat gaagtcctgg gctgaaatgc
     3481 gggaccaggg actgccccgc gccgagcgct tgctgcgagt cgccaagctt ggcgggttct
     3541 atgtgcctgc gctctaccaa agcgtgcccg tcgaggagac gagaatgcag gtcgtggagc
     3601 cgatccaccc cgaggcgccc ttccctgtcc ggcgctcgat cgtgccggat ctcaacgaat
     3661 acccgttccc ggtagatgga ccgatcgcca attgtcagac cgtcttcgac cgggcatcga
     3721 tcgaggtcgc gcgtggctgc accgaggggt gtcgattttg ccaggccgga atgatctacc
     3781 gccctgtccg cgaacgcgag ccggatgcga tcatcgacgc gatgctgcgc tcggttgccg
     3841 aagcggggta cgacgaagcc tctctcacat ctctttccac agccgattac agcgcgatcg
     3901 cgccactcgt gcaccagtcg atcgacgctc tcgccccgta tcaagtctcg ctgagcgtat
     3961 cctcgcttcg cgcgtacgga ctgagcgagg acgtgctcga cgacatgaag cgccaacgcg
     4021 caggaggtct cacgttcgct ccggaagctg gtagtcagcg gatgcgggac gtggtcaaca
     4081 agaacgtgac cgacgcgcag ctcatggaaa ccgccgaacg agtcttctcc cgcggctgga
     4141 gcaagatgaa gctctatttc atgatcggac tgcccaccga agaggacgag gatgtccgag
     4201 acatcgttcg caccggggcc cgtgcactcg aagtcgcccg gcgcgtgcaa cgaaacaaac
     4261 gtgcgcgggt taccgtgagc gtgtccaccc acgttccaaa gccgcatacg ccgtttcaat
     4321 ggtgcgccat ggactcgcaa gacgaaatca agcgcaagca gtcggttttg cgcaatgaag
     4381 cgtcgagctt tcgcatacag ctcaaaatgc acaactcgga cggctcgtgg ctcgaaggca
     4441 tgctcgcacg gggagaccga cgtctggccg atgtcattca gggtgcctac gttcggggcg
     4501 cacgattcga ctcctgggac gagagactcg acctcgccca ttggacggat tccatcgagg
     4561 atgcaggaat cgaccctgcc tggtttctgg ggacgattcc tgtctcggcg cagttgccct
     4621 ggtcacacat cgacgtcggg ctcgagcagg gcttcctggc gcgcgagtac cgcaagtcgc
     4681 tccgcaatcg cctgagcccc ccatgcggca agaccgtcgg cagcttcgtc catcacacga
     4741 acgtgaaaga agcgaacgac gagaagcgcc gactggtttg ctacgactgc ggcatcgcct
     4801 gcgacctcag cgaaatgaag gacgagcgca tccgtttcct cgaaagcatg aacgcgttcg
     4861 agccgaggaa gacacctgct ccggatgagc cggctgatga agacaacgtc gtcgacaggc
     4921 gtccgtttta cggagtcgac cagggagcac cgatgcgcat tcggatcgga tatcgcaaga
     4981 tcggtcgaac cgcgtacacc tcccacctcg acttggtgcg cgcctttcct cgcatgcttc
     5041 gacgcgtcgg gcttccgctc ttctacagcg agggcttcag gcccttgcct aaattgactt
     5101 tcggacccgc tcttccggtc ggcacagcga gcctttgcga gcacgtcgac gttcggcttc
     5161 gcgccgacga gcaccccgac ctgaaccgcc tctgcgcgcg tctgaacgat gcggcgatcg
     5221 atgggatcga atttttcgag caccgcgtcc tcggcccgcg ggacgcagcc ctcaaccggg
     5281 tgatcgagga agcggactac gtcgctgcgt tctcgaggca ggagcttcga tcgctcggca
     5341 tcgaggacga ggacaccctg gcgcgcatgc tcagtgagcg cccgagcgag ctcttcatcg
     5401 accgggaggt ccaaggagtg aagaagcgaa tcgacgtcgg ggccgccctg gtcgacctcc
     5461 gcgtcggcga aggtcggaac gctctcgcgc tggccgggtt ggctggggac ctgctgccga
     5521 ttcgcttgac ccttcgtatt gggggaaaga cgacgccaag gcccggggaa gcgcttcgtg
     5581 ccctcaccgg actggacgag ctcgtccctc gcgtcgtccg atgcggctta ttcgcgaaca
     5641 ggccgactgg acgggtcgcg ccgttgcagc tggagctcct ccgggtcaaa cctacccttc
     5701 aggccgccga atgacagacc gtttcgacat cgtccgaagc ttggtacgcg cagcggccga
     5761 tcgcgctgtc gatcgccttg cgagcacccg agtcgccgcc gccacgaagc aactcgtcga
     5821 acgtctcacg aagcagcgct ggaggctcag cgacgaaacg ctcacggcag ccgccgcgca
     5881 tgcgcacgga gtcgattcag cgatggtcgc ctgccattcc gggcgcctct tcgtcgacgc
     5941 ctcgatgacc aacggaagag aagtccagtt cagtttgacc ccgttctccg ttcgcttcgc
     6001 gccacgcggg gccaaagaga tctcgttcga cgtcgatcca cccgacgctc acgacagctc
     6061 gatcgtggga gcgctggctg gatgcatcgc caaggcgacc tggcccatga tcgcctcttt
     6121 ggacgctgtc gacatgggag gtgccatcgt cgagcgcgag agcccaggac gactgagggt
     6181 ggacctgcga agcgtaccgg cagtgcggaa gtttgcatct tcccagaccg caacgatgat
     6241 cttcgacgtt ctcgagctcg agtcgatacg ggtcgagccg ggcgggctcg ctctaaagct
     6301 caagctaccg cagttgacac cctagagctc ttctcgccaa cgctgcgaca gtgccacttg
     6361 ggccttgtag tcgtcgatct gcttttgttt ctcctcgtca gaccagccga tcagctcggc
     6421 catccgggag gccacctttt cggccgagcc gagcccctgc cgggagtcgc gaaagaaaat
     6481 ctgcgtacgc cgaatcatga tgtcgctcag gctggccgcg agctcttcca gtacgccgaa
     6541 atccacctgc gccatgatct ctacgcgccc ggggatgatc gcttcgatca aggaaggatc
     6601 agcggcgcag agcttggcga gctccaaggc gcgcatcccg taggtgtcga ccaggtgaag
     6661 cctggctttg tcggagaggt cgcatttaca caaccgaccg atctcgtccg cgactttctg
     6721 atggtcgtcg tcctcgggcc aacccaccgc ccccggcaga gggaacttga aggtctgccc
     6781 ggagtgaatg tcttctggaa gcttgccgga aagcttcagc aagttgaccg caacgtcgac
     6841 gcactctttg gccatcttgc ggtaggtcgt gagcttcccc ccagcgatcg tgatcaagcc
     6901 atcttcgccg atcatgatct gatgttcgcg gctcacctgt gactcggcca tctcgcctac
     6961 ctcaggctct ggagcgatga gcgggcggag cccagcccaa gtcgagatca cgtcgtcgcg
     7021 ggagatctgc ttagttggga agtaatagtt cgcggcggcg atcagataat ccacgtcttc
     7081 gagcgtcgcg gcttcctcgc caggtgcgcc gtcgtagtct gtgtccgtcg tgccgacgta
     7141 ggtgcgctct ccccagggca gcgcaaaaag cacccgtttg tccgtcggat ggaacaagac
     7201 gactgcatgc tccaccggaa gcttttctcg gtcaacgacg atatggatgc ccttggtggg
     7261 ccggagcact ttgccctttc gcgcgccact catcgcaagg acctcgtccg tccaaggacc
     7321 ggtggcgttg atgaccgtat gcgccttgac ctcgcgaagg cttccgtcgc gaacgttctt
     7381 gaccacgaca cccgaaacac ggccctgctc gttcttggtc agcgcgtcta ctcgcaccca
     7441 gttcaccgca acgccaccgt tccggattgc atcgatgacc gtttcgagcg tcagtcgcgc
     7501 atcgtccgtg gagcagtcgt agtaaaccgg cgcaccttgc agg
//
LOCUS       DASRZF010000375         6012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2696275, whole genome shotgun sequence.
ACCESSION   DASRZF010000375 DASRZF010000000
VERSION     DASRZF010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 6012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6012
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2696275"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VFG22_16575"
     CDS             complement(<1..335)
                     /locus_tag="VFG22_16575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417914.1"
                     /translation="MAEFDIFNGDADGICALTQLRNAEPLESTLITGVKRDIELVVKA
                     DLSEGDRATVLDISFDKNREAVSKALDAGAEVFYVDHHFAGAIPEHEKLSTIIDPDPN
                     VCTSLLVNG"
     gene            complement(344..1090)
                     /locus_tag="VFG22_16580"
     CDS             complement(344..1090)
                     /locus_tag="VFG22_16580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET6417915.1"
                     /translation="MIECSGVLDGRTIAVVVPAYNEAELIRDTLGGLPAFVDQIIVVD
                     DASTDATGELVKAFFPSVVLLSHDANRGVGAAIATGCRRALALGADFTAIMAGDGQMH
                     PDDLPALLQPLIAEEVDFTKGNRLAWPSVRSEMPWHRRVGNQLFSSLTRQALGIDVHD
                     SQCGYAAMSRRASLAIEWDRLWRGYGYPNDLLSCLSLLGLRVRDVPVRPIYGAERSGI
                     RLRHVACIIPFVIIRGWLRRTALWPTSLVL"
     gene            1128..2156
                     /locus_tag="VFG22_16585"
     CDS             1128..2156
                     /locus_tag="VFG22_16585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6417916.1"
                     /translation="MAQVLFVSKPVVPPWNDSSKNLVRDIAASLERHSPVLVERSRHL
                     GIFRQLLFATSPDIWHFFFAPNVKTSAAARFARAVRRVPSVHTVCSLPAEQDAIERLL
                     FADVTVALSRAAFERLRLAGVDETALRLIPPCVPPLSEPGPDRRGELRKKHAIPENTQ
                     VWIYPGDLEFGGGAEIAVKGFAAWNRPDALLLIACRPKTARAAVERLRLMDLARRWGI
                     ASQVRWLGDTLQIHELLTLSDFVVLVNRSAYAKMDYPLVALESMCLARPVLVGRGTPS
                     EELAELGAAVAVEPDAEALADAVRRLSADEASLIELGHRAHSVATSTFSPKQVAAAYE
                     RLYEEIRA"
     gene            2149..2871
                     /locus_tag="VFG22_16590"
     CDS             2149..2871
                     /locus_tag="VFG22_16590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6417917.1"
                     /translation="MPDASEREVQRYYDDFSDSYERGRGSGYHRMIDDLEFEVAAQFA
                     EGARVLELGCGTGLILERIAAIANEAVGIDLSERMAQRARERGLEVFLGSLSELPFAN
                     EQFDLTYGFKVLPHVADIETAIGEAVRVTRPGGHLLLEFYNPWSVRRLAKRLAGPAPI
                     SERRTEADVYTRWDSPRAIRRLMPPDLVLLELRGVRVLTPFAAIHGLPVLGPGLRWAE
                     RSAGSSPLRYFGGFIIAVLRKR"
     gene            2925..3626
                     /locus_tag="VFG22_16595"
     CDS             2925..3626
                     /locus_tag="VFG22_16595"
                     /inference="COORDINATES: protein motif:HMM:NF044362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417918.1"
                     /translation="MAPARVTSPDRDVLFEARGVRQQVLHAALLIAVVAGGLAFARTV
                     IDAIERDAWRVVGEAVVMYAGFVALLFARRLPYKARALSFLGLLYVVGVVALLSVGYL
                     AAPVLVLAGQSVLASVLFGRRVTLIALAMNLLALLGVGAALSTGIATVETTAFYEPSL
                     FVNWLRVTAIFAVFCGIAVVSVDVITNHLNQSLRDQAELIENLKGAMQLRDESERQRR
                     AAESRLRDASKRAED"
     gene            complement(3674..4183)
                     /locus_tag="VFG22_16600"
     CDS             complement(3674..4183)
                     /locus_tag="VFG22_16600"
                     /inference="COORDINATES: protein motif:HMM:NF017090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417919.1"
                     /translation="MTASSNGLPLAQRKKDIFFVVCFSFFAFSSFFSDSWHALGLLEG
                     DAFWSRANRWYGEIAQDHFFLADHQFVRVNTGISGMIYGPFYLLLVFAFIKGKNWIRP
                     MALVYVGAMLHGCTEFLIYEYWIGPPPGNALVFWAFNGPYWVVPFLLGVRMWKPDPFG
                     TPAELGARS"
     gene            complement(4217..5101)
                     /locus_tag="VFG22_16605"
     CDS             complement(4217..5101)
                     /locus_tag="VFG22_16605"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET6417920.1"
                     /translation="MPAEFDWEKLQVFVAAADRGSLSGAARTLGISQPTAGRQVQALE
                     SQLGVQLFSRTARGLALTDTGMELLGYAREMSDAANRLALAALGRVEALGGVVRITAS
                     EIVATYRLPPVLLALRAAEPEIEVELVASNATDNLLQREADIAVRMYRPSQTDVITRS
                     LGEIELGVYAAESYLARRGCPRTPEDLLEHDVIGYDRDESMIRGFRSAGVTVERRFFP
                     IRTDDQVVAWRMVVEGNGIGFMQVAIGDAEPAVRRILPDLPLPTLPIWLTAHSELKTS
                     RPIRRVYDFIADSLRVAL"
     gene            5219..>6012
                     /locus_tag="VFG22_16610"
     CDS             5219..>6012
                     /locus_tag="VFG22_16610"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HET6417921.1"
                     /translation="MEKEVLVVGANGRIGRWSVEAFLDAGWKVRAFVRTGSSARVRGG
                     VIVFEGDAFDAEAVTAASEGVAVIVNALNPPYERWAEEVPKITQSILSAAEASGATVM
                     LPGNVYNYGDGMPPVLREDTPHVPTTRKGRLREEMEEAYRQASARGVQTIVLRAGDFI
                     ERKATGNWFDTYVGNKVEKGIVTYPGPLDRVHAWAYLPDVARAMVGLAAVRESLGAFE
                     TIGFGGFSLTGAELVETMERALERPLKLKRFPWGLVRLMGPFSAPMR"
BASE COUNT         1135 a         1843 c         1922 g         1112 t
ORIGIN      
        1 tagccgttga cgagaaggct cgtgcagacg tttggatcgg ggtcgatgat cgtgctgagc
       61 ttttcgtgct ctggaatggc gccggcaaag tggtggtcga cgtagaaaac ctccgcgccg
      121 gcatccaacg ccttcgacac ggcttcccga ttcttgtcga agctgatgtc gagaaccgtc
      181 gctcgatcgc cctcgctcag gtcggccttg acgaccaatt cgatgtctcg cttcacgccg
      241 gtgatgagcg tactctccaa aggctctgcg ttgcgaagct gggtaagcgc acagatgcca
      301 tcggcgtcgc cattgaagat gtcgaactcg gccatggacg cgtctatagc accaatgacg
      361 ttggccaaag cgcggtcctc ctcaaccagc cgcgaatgat cacgaatgga atgatgcacg
      421 ccacgtgacg aagtcgaatt ccgctgcgct cggcgccgta gatcggccgt accggcacat
      481 cccggacgcg caagccaagg agcgacaagc aactcagtag atcattcgga tacccgtagc
      541 cccgccagag tcgatcccat tcgatcgcga gcgaggcgcg tcggctcatg gcggcgtagc
      601 cgcactggga gtcgtggaca tcgattccaa gcgcctggcg cgtcaaggaa gagaagagct
      661 ggttcccaac ccggcgatgc cagggcatct cgcttctgac cgacggccaa gcaagccggt
      721 tccctttggt aaaatctacc tcttcggcga tgaggggctg caacaaggcg ggtagatcat
      781 cggggtgcat ctgtccgtcg ccggccatga tggcggtgaa atccgcgcca agcgccagcg
      841 cacgcctgca gcccgtcgcg atcgcggccc cgaccccccg gttggcatcg tgcgagagga
      901 gcacgaccga cggaaagaac gctttcacta gctcccccgt ggcatcggtg ctggcgtcat
      961 ccacgacgat gatttgatcc acgaaggctg gaaggccccc tagcgtatcg cgaatcagct
     1021 cggcttcgtt gtaggcgggc acgacgaccg cgatggttct tccatccagc actccggaac
     1081 actcaatcac ttgctccgag tggtcgcaag gcgtacctca attgccaatg gcgcaagtcc
     1141 tcttcgtttc gaagccggtc gtcccgcctt ggaacgacag cagcaagaac ctggttcgcg
     1201 acatcgcagc cagcctagag cgtcattcac ccgtgctggt ggaacgttct cgccatctag
     1261 gcatcttccg ccaactgctg ttcgcgactt cgccagacat ctggcacttc ttcttcgctc
     1321 ccaacgtaaa gacctccgcg gcggcgcgct ttgcacgcgc ggtccgaagg gtgcctagtg
     1381 ttcacaccgt ctgcagcctg cctgcggaac aagacgccat tgaaaggttg ctattcgcgg
     1441 acgtgactgt ggcgctttcg cgagccgcat tcgagcgctt gcgtctggca ggggtcgacg
     1501 agaccgcgct ccggctgatt ccaccttgcg tgcctccgct gtccgaaccg ggcccggaca
     1561 ggcgcggcga gctgcgcaaa aagcacgcga taccggagaa cacgcaggtc tggatctatc
     1621 ctggagacct cgagttcggc ggtggagcag agatcgccgt gaagggcttc gcagcatgga
     1681 atcgtccgga cgcgctactc ctgatcgctt gtcgcccgaa gacggcccgc gccgccgtgg
     1741 agcgtctccg gctcatggac ctggcgcggc gatggggcat cgcttctcag gtgcgatggc
     1801 tgggagatac tctgcagatc cacgagctgc ttaccctcag cgacttcgtg gtgctggtga
     1861 atcgcagcgc ctacgcgaag atggactacc ccctcgtcgc gctcgaatcc atgtgcctgg
     1921 cccggcctgt tctggtcggt cggggaacgc catcggagga gcttgccgag ctgggggcag
     1981 cggtcgcagt cgaacccgac gcagaggcac tcgccgacgc agtgaggcgc ttgagtgccg
     2041 acgaggcatc gctcatcgag ctcggacata gggcacacag cgtggcgact tcgacgtttt
     2101 ccccaaagca agtcgccgca gcgtacgagc gtctttacga ggaaatccgt gcctgacgca
     2161 tccgagcggg aggttcaacg atactacgac gatttcagcg actcgtacga acgagggcga
     2221 ggctcaggct accaccggat gatcgatgat ttggagttcg aggtcgccgc gcagttcgcc
     2281 gagggcgctc gggttctgga gctcggctgc ggcactggtc tgatcctcga acgtatcgcg
     2341 gcaatcgcga atgaggctgt cgggatcgat ctgtcggagc gtatggcgca gcgggcgaga
     2401 gagcgtgggc tcgaagtttt cctcggaagc ctcagcgagc ttcccttcgc caacgagcag
     2461 ttcgacctca cctacggctt caaggtcctc ccgcacgtcg cggacatcga gacggccatc
     2521 ggagaagcgg tgcgggtgac tcgtccgggc ggacacctct tgctcgagtt ctacaaccca
     2581 tggagcgttc gccgtctggc gaagaggctc gcggggccgg ctccgatcag cgagaggcgg
     2641 acggaagccg atgtctatac gcgctgggac tcgccccgag cgatacgaag actcatgcca
     2701 ccggacttgg ttctgctcga gctccgaggg gttcgggttc tcactccctt cgcggccatt
     2761 cacggtctcc cagtgctcgg acccggcctg agatgggcgg agcggtctgc aggaagctcg
     2821 ccacttcggt actttggcgg cttcatcatc gccgtgctgc gcaagcgcta gaagcggcaa
     2881 cgtggcctgg agctacgctc gctggcagag tagcattgcc agcagtggca cccgcacgcg
     2941 ttacctctcc agatcgagac gtcctcttcg aggccagagg cgttcgtcag caagtcctgc
     3001 acgcggcact gctgatcgcc gtcgtcgccg gaggtttggc cttcgcccga accgtgatcg
     3061 acgcaatcga gcgggatgcc tggagggtcg taggggaagc cgtggtgatg tacgccggct
     3121 tcgtcgccct gctctttgcg cggcgtcttc cctacaaagc tcgagctctc agcttcctcg
     3181 gcctgctcta cgtcgtgggc gtcgtcgctc tcttgtccgt cggctatcta gcggccccgg
     3241 tcctggtctt ggcgggccaa agtgtcctgg cgtcggttct gttcggacgc agggttaccc
     3301 tgatcgcgct agcgatgaac ctgctcgcac ttctgggagt gggagcggcg ctttcaacgg
     3361 gtatcgccac ggtggagacg accgccttct acgagccgtc gttgttcgtg aactggctgc
     3421 gcgtaactgc gatctttgcc gtgttctgcg gcatcgctgt cgtaagtgtc gacgtgatca
     3481 ccaatcactt gaaccagagc ctgcgcgacc aagccgagct gatcgagaat ctcaagggtg
     3541 cgatgcagct gcgcgacgaa tcggaacgtc aacgtcgggc cgccgagtcg cgcctccgcg
     3601 atgccagtaa gcgcgcagaa gactgagtcg gaggttcttg aacccgtccg agccgggaat
     3661 cgcgccccgc ctctcagctt cgagccccca gctcagcggg ggtcccgaat ggatcgggct
     3721 tccacatgcg cacgcccagc aagaacggaa ccacccagta tgggccgttg aaggcccaga
     3781 aaacgagcgc gttgcccgga ggaggtccga tccagtactc gtagatcagg aactccgtgc
     3841 agccgtgcaa catcgcaccc acgtagacca gcgccatcgg ccgaatccag ttctttccct
     3901 tgatgaacgc gaagacgagc aggagataga agggtccgta gatcatgccc gatatgcccg
     3961 tgttgacccg aacgaactgg tgatccgcga ggaaaaaatg atcctgtgcg atctcgccgt
     4021 accagcggtt cgcgcgcgac cagaacgcgt cgccttcgag aagaccgagc gcgtgccagg
     4081 aatccgagaa gaacgaggag aacgcaaaga acgaaaagca aacgacgaaa aagatgtctt
     4141 tctttcgttg tgcaagcggc aggccattac tcgatgcggt cattttctct cctcaaagac
     4201 ggcgccgtgt ataccatcaa agcgctacgc gaagcgagtc tgcaatgaaa tcgtagactc
     4261 gacggatggg gcggctggtc ttgagctcgg agtgcgcggt gagccagatg ggaagcgtgg
     4321 gtagcgggag atccgggagg attcggcgca cggcaggctc cgcatccccg atggcgacct
     4381 gcatgaatcc gatgccgttc ccttcgacta ccatgcgcca ggcgacgact tgatcgtcgg
     4441 ttcggattgg aaagaagcga cgttcgacgg tcaccccagc cgaacgaaac ccgcggatca
     4501 tcgactcatc acggtcgtat ccgatcacgt cgtgctcgag gaggtcctcg ggggttcttg
     4561 gacagccgcg tcgggcgaga tagctttcgg ccgcatacac gccgagctcg atctcgccga
     4621 ggctgcgggt gatgacgtcg gtctgcgagg gtcgatacat gcgaaccgcg atgtcggctt
     4681 cgcgctgcag gaggttgtcc gtcgcgttcg aggcgaccaa ctcgacctcg atctcgggct
     4741 ccgcggcccg aagggcgagc aagaccggtg gcagtctgta cgtcgcgacg atctcgctgg
     4801 cggtgatgcg cacgacgcct cccagcgctt cgactctgcc aagcgcggcc aaggccaggc
     4861 ggttggcggc gtcagacatt tcccgggcgt acccgagtag ctccatcccc gtatccgtga
     4921 gcgcgagacc gcgcgccgtc cggctgaaga gctggacacc caactgcgac tcgagcgcct
     4981 ggacctgccg tccagccgta ggctggctga tcccgagagt gcgtgcggcg cccgaaagcg
     5041 agccccggtc ggcggcagcc acgaagactt ggagcttttc ccagtcgaac tcagcaggca
     5101 tctaggtacc tatgcattat tgcatatcta tattcgatat ttggcggatt taccatcatt
     5161 ttcgcatgcg tatgattcag accgaaccgc agcgaactgc ggcaaggagc agggcagcat
     5221 ggaaaaagaa gtactggtcg tgggcgccaa tgggcgcatc ggacgctggt cggtggaggc
     5281 gttcctggac gcgggatgga aggtgcgggc cttcgtgcgg accggttctt ccgcgcgtgt
     5341 acggggcggc gtaatcgtct tcgaggggga tgcgttcgac gctgaggccg ttacggcggc
     5401 gtccgagggc gtcgcggtca tcgtgaacgc gctcaaccct ccctacgaac gctgggcgga
     5461 ggaagttccc aagatcacgc agagcatcct gagcgctgcc gaggccagcg gggccacggt
     5521 gatgctaccg ggcaacgtat acaactacgg ggatggcatg cctcccgtcc tgcgcgaaga
     5581 cacgccgcac gtcccgacga cccgcaaagg gcgcctccgc gaggaaatgg aagaggcgta
     5641 ccgtcaggcg agcgcgcgcg gcgtacaaac gatcgtgctt cgcgccgggg acttcatcga
     5701 gcgcaaggcg acgggcaact ggttcgatac ttatgtcgga aacaaagtcg aaaaagggat
     5761 cgtgacgtat ccagggcctc tcgaccgcgt tcacgcatgg gcctacttgc cggacgtggc
     5821 ccgggcgatg gtcggcctgg cggcggttcg cgaaagcctc ggcgccttcg aaacgatcgg
     5881 cttcggtggc ttttcgctta ccggggccga gctcgtagaa accatggagc gcgcccttga
     5941 gcgcccgttg aagctcaagc gatttccctg gggactggtt cgattgatgg gaccgttcag
     6001 tgcgcccatg cg
//
LOCUS       DASRZF010000376         5465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_5097164, whole genome shotgun sequence.
ACCESSION   DASRZF010000376 DASRZF010000000
VERSION     DASRZF010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 5465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5465
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5097164"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..1078)
                     /locus_tag="VFG22_16615"
     CDS             complement(164..1078)
                     /locus_tag="VFG22_16615"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HET6417922.1"
                     /translation="MSRGFVVSRSSIALAVLALGFLPSLASALEVPTLSGRVNDYAGI
                     ISPEAEVRIEQTLAALERDKGAQVVVLTIEALDGEQLEDYSLRVAESWKLGRADQDDG
                     ALLFIVKNDRKMRLEVGYGLEPYLSDVMSRRILDQILRPSFRAGDFDGGIERAVEAID
                     GLIRGTSTLPAPEPSPFEGDLPTQMFGLLWFLFLIPFVFTVLGTNPFQWFLYLFLIPF
                     VYVGGLTLLGPNASPVFVAAWLIGAPIVWSFFGRRRKKRKPGTRRRGSGWSGGSWSSG
                     GGGFSSSGGGGFSGGGGSFGGGGSSSSW"
     gene            complement(1053..1709)
                     /locus_tag="VFG22_16620"
     CDS             complement(1053..1709)
                     /locus_tag="VFG22_16620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HET6417923.1"
                     /translation="MQGHIETFFSEADREAIRAATTAAERNTAGEIVVYVVERCDLYP
                     ELAWKGALVGGALGAMCAALGAWWFGGWGAADHLGILIGLQVGLVLGWVASRFEPVAR
                     RLIGDELLESRVEGRAAEAFLEEQVFATRERSGVLIFVALFEHRVLILADQGIDACVE
                     ESAWAAISNEVAAGVRRRKPAEALIDAVQRCADLLVEHGMSPKDSKNELSNEPRFRRE
                     "
     gene            complement(1718..2305)
                     /locus_tag="VFG22_16625"
     CDS             complement(1718..2305)
                     /locus_tag="VFG22_16625"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HET6417924.1"
                     /translation="MSKGVIGCGVIVAIALALLATGVGSYNRLVELDEGVDNAWSQVE
                     NVYQRRADLIPNLVATVKGAKDFEQETLTKVVEARAKVGQVNLSSAPDAEQLAQFEAA
                     QSQLSSALSRLLVVVERYPELKATEAFRDLQAQLEGTENRITVERMRFNDATLAYNKT
                     RRRFPTVLLAKIMGFDDKPYFESTPGAEEPPKVEF"
     gene            complement(2375..3781)
                     /locus_tag="VFG22_16630"
     CDS             complement(2375..3781)
                     /locus_tag="VFG22_16630"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6417925.1"
                     /translation="MRVEFVGAVGGNVTGSRHVVHTEHARVLLDCGLFQGRRAETVER
                     NRSLGFRAKDIDVVVLSHAHIDHSGALPRLYRQGFRGPIYCTPATRDLCAAMLEDSAE
                     IQAQDSAYINRKISREGAKMDPVEPLYEQKDVVGTLELMVGIPYHRPQKIAEGVTLTY
                     YDAGHVLGSAMVALDVDVVGEIRRLVFSGDLGRRNMPLLRDPEIPPNASFLLMESTYG
                     DRTHDPIELMEEELLAVVTRTVERGGKVIVPSFALERAQEVVYSLKKLKDRGRLPDVP
                     VFVDSPLTVKITDVFRVHPDCLDREAQGTLLQGGSPFDFDGLRYVSSVEDSKAIDRSE
                     EPCIIISASGMCEFGRVVHHLANAIGSRDNTVMIVGFQAQHTLGRRLVEQRPRVRIFG
                     VERERHAEVVVLNGFSAHAGQDDLLEYAEAVREAGPLRQVALVHGEDPARKTLKGLLE
                     ERGFPSVHTPSQGDWLDL"
     gene            complement(3771..4523)
                     /locus_tag="VFG22_16635"
     CDS             complement(3771..4523)
                     /locus_tag="VFG22_16635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6417926.1"
                     /translation="MALFELRGLRKAFGRQLVLDGLDLDIVEGEVITIIGESGSGKSI
                     LLKAMMGLIEVDAGTIRFDGEDVTHQTEAEWNQTRRRIGMLFQESALFDSLDVYENVA
                     YGLREQTSLSEEEIVQRVAETLALVALPGIESMAPSDLSGGMRKRVALARAVAVRPAV
                     VFYDEPAEGLDPINVTRVNRLLLGLQKRLNVTSVVVTHNLKAAFAISDRLALIHDGVV
                     AVTGSPDAFLESEDPVVREFIGERMMRVLRAG"
     gene            complement(4536..4856)
                     /locus_tag="VFG22_16640"
     CDS             complement(4536..4856)
                     /locus_tag="VFG22_16640"
                     /inference="COORDINATES: protein motif:HMM:NF013517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-potential iron-sulfur protein"
                     /protein_id="HET6417927.1"
                     /translation="MKKTTISRREMLGQSVRLVVVGSVPVLLNACTKPELHCQDVSGL
                     SETDMELRTQLEYRDDSPYGEKKRCSTCAFYKAGKKDECGQCTLVKGPIHPLGYCNSW
                     SAKA"
     gene            complement(4898..>5465)
                     /locus_tag="VFG22_16645"
     CDS             complement(4898..>5465)
                     /locus_tag="VFG22_16645"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET6417928.1"
                     /translation="RGVLPYPRPAVRDPMWLAVAFGLLAGGVITGAIHLSFASGAWGA
                     RFRKALGVSACVAGLLGIVGWAEALPAGEHIRWVESFDLGKAKAVAEGSPMLVDFGAD
                     WCGACQELERDVLSDPRIVSEARRFVTIRVDLSTDQATDDEWTLLKAYNQPGLPLVVL
                     HNSDGSEASRITGLVESEAFLDLMKAVR"
BASE COUNT         1032 a         1844 c         1608 g          981 t
ORIGIN      
        1 ggccttcgcc tgaggcgacc accgaccaag ccgaagtggc tccctgtgat tgcatttcgg
       61 gtcgacccgg cgagctcgat ccccgaggcc tgggcgaact gggtctactg gcacggccgt
      121 ctcgacagcg atccctaaaa cccggcagct gcgcagcagc cgttcaccag ctgctcgacg
      181 agcccccgcc gccgaagctt cccccgccac cggagaaccc gcctccgccc gagctcgaaa
      241 agcctccgcc acccgaggac caagagcccc cggaccatcc cgagccgcgc ctgcgcgtgc
      301 ccggcttccg cttctttcgc cgccgcccga agaacgacca gacgatcggg gccccgatca
      361 gccaagccgc gacgaagacc gggctcgcgt tcggtccgag cagcgtcagt ccaccgacgt
      421 acacgaacgg gatcaagaac aaatagagga accactggaa cgggttcgtg ccgagtacag
      481 tgaagacgaa ggggatgaga aacaagaacc agagcaggcc aaacatctga gtcggcaagt
      541 cgccttcaaa cggactcggc tctggggccg gaagcgtgct cgtcccgcga atcagcccgt
      601 cgatcgcctc gaccgcgcgc tcgatgcctc cgtcgaagtc gcccgcgcga aagctcggcc
      661 tcaagatctg atccaagatc cgcctcgaca tgacgtcgct cagatacggc tcgagtccgt
      721 agccgacttc gagtcgcatc tttcggtcgt tcttgacgat gaagagaagc gctccgtcat
      781 cttggtcggc acggccgagc ttccagctct ccgcgacgcg caagctgtag tcctcgagct
      841 gctccccatc gagcgcttcg atggtgagaa cgaccacttg cgcgcccttg tctcgctcga
      901 gcgcagcgag cgtttgctcg attcggacct cggcttccgg cgaaatgatc cccgcatagt
      961 cgttgacgcg accgctgagc gtgggcacct cgagcgccga tgccaagctg ggcaagaaac
     1021 caagcgccag cacagccagc gcgatgctgc ttctactcac gacgaaacct cggctcattc
     1081 gagagctcgt tcttcgagtc tttgggcgac atcccgtgct cgacgagaag atctgcgcag
     1141 cgctgaaccg catcgatcag cgcctccgcc ggctttcggc gccgcactcc ggccgccact
     1201 tcgttcgaga tggcagccca ggcgctctct tccacgcacg cgtcgatccc ctggtcggcg
     1261 agaatcagca ctcgatgctc gaacagtgcg acgaaaatca gaacaccgct tcgctcgcga
     1321 gtcgcaaaca cctgctcctc cagaaacgcc tccgctgcac gcccttcgac gcggctctcg
     1381 agcaattcgt ctccgatcaa gcggcgagcg accggctcga agcgactcgc gacccagccg
     1441 aggacgagac cgacttgcaa gccgatcaag atccctaggt gatccgccgc gccccagcct
     1501 ccgaaccacc aagctccgag ggcagcgcac atggcgccga gcgccccgcc gaccagcgca
     1561 cccttccaag cgagctcggg atacaggtcg cagcgctcca ctacgtagac cacgatctcg
     1621 ccagccgtgt tccgctcggc ggccgtggtg gcggcgcgga tcgcctcacg atcggcttcg
     1681 gagaaaaagg tttcgatgtg cccctgcacg gcgggggcta aaactcgacc ttcgggggtt
     1741 cctctgcccc gggcgtcgac tcgaagtagg gcttgtcgtc gaagcccatg atcttcgcga
     1801 ggagaaccgt cggaaagcgc cgtcgcgtct tgttgtaagc gagcgtcgcg tcgttgaagc
     1861 gcattcgttc gaccgtaatc ctgttctctg tcccttcgag ctgagcctga aggtctcgaa
     1921 atgcctccgt tgccttcaac tcgggataac gctcgaccac taccagcagt cgggagagcg
     1981 cggaggagag ttgggactga gcagcctcga actgggcgag ctgctcggca tccggcgcag
     2041 agctcagatt cacttggccg actttcgcgc gcgcttctac gaccttcgtg agggtctcct
     2101 gttcgaagtc cttcgcgcct ttgacggtcg ctaccaagtt cggaatcaga tcggcgcgcc
     2161 gctgatagac gttctcgacc tggctccagg cgttgtccac cccttcgtcg agctcgacca
     2221 aacggttgta gctacccaca cccgtggcca gcagcgccaa cgcgattgcc acaatcacac
     2281 cacagccgat cacgcccttg ctcatgaacc ctcctcgttt ctcacgcgct cggagaatgc
     2341 gcgtagaccc tgcacgcggt caaggcctcc gctttcagag atcgagccaa tcgccttggc
     2401 tcggcgtgtg aacactcgga aatccgcgct cttcgagcag ccccttgagc gtctttcgcg
     2461 ctggatcttc gccatgaacg agggcgactt gccgaagcgg gcctgcttcc cgcaccgcct
     2521 cggcgtattc gagcagatcg tcctgcccgg catgcgcgct gaacccattg agcacgacga
     2581 cttccgcgtg ccgctcacgc tccacgccaa agatccgcac ccgaggccgt tgctcgacca
     2641 ggcggcgtcc gagcgtgtgc tgcgcttgaa agccgacgat catcacggtg ttgtcgcggc
     2701 ttccgatcgc gttggccagg tgatggacca cccggccgaa ctcgcacatt ccactcgccg
     2761 aaatgatgat gcaaggctct tcgctccggt cgatagcctt cgagtcctcg accgaggaga
     2821 cgtaacgcag cccgtcgaag tcgaacggag atcccccttg gagcaaggtc ccctgcgctt
     2881 cgcgatccag gcagtccggg tgaactcgaa acacgtccgt gatcttcacg gtcagaggcg
     2941 agtcgacgaa tacggggaca tctggcaatc gtccccggtc tttgagcttt ttgagcgagt
     3001 agacgacttc ctgggcgcgc tcgagcgcaa acgaaggcac gatgaccttg cctccacgct
     3061 cgacggttcg cgtgaccacg gcgagaagct cttcttccat gagctcgatc gggtcgtgcg
     3121 ttcggtcccc gtacgtgctt tccatcaaga ggaacgaggc attcgggggg atctccggat
     3181 cgcgcagcag aggcatgttg cgccgtccga ggtcacccga aaacaccagg cgccggattt
     3241 ctccaacgac atcgacatcg agcgctacca tcgcgcttcc gagcacgtgc ccggcgtcgt
     3301 agtaggtcag cgtgacgccc tcggcgatct tctgcggtcg atgatacgga atgcccacca
     3361 tgagctcgag cgttcccacc acgtcctttt gctcgtacag gggctcgacc ggatccatct
     3421 tcgcgccctc tctcgagatc ttgcgattga tgtacgccga atcctgcgcc tgaatctccg
     3481 ccgagtcttc gagcatggcc gcgcagagat cccgagtcgc aggggtgcag tagatcggtc
     3541 cgcgaaagcc ttgacgatag agacggggga gtgcgcccga gtggtcgatg tgggcatggg
     3601 aaagcaccac cacgtcgatg tctttggcgc gaaagccgag gcttcgattg cgttccaccg
     3661 tttctgcgcg acgaccctgg aacaaaccgc agtcgagcag cacgcgagcg tgttcggtgt
     3721 gcacgacgtg ccgtgagccg gtgacgtttc ccccgaccgc gccgacgaat tcaacccgca
     3781 cgcagcactc gcatcattcg ttcgccgatg aactctcgca ccaccgggtc ctccgactcg
     3841 agaaacgcgt cgggtgagcc cgtaaccgca accacgccgt cgtgaatgag cgcgagccgg
     3901 tcgctgattg caaacgccgc cttgaggttg tgcgtcacga cgaccgacgt gacgttgagc
     3961 cgcttctgaa gcccgagaag cagtcggttg actcgcgtga cgttgatcgg atccagacct
     4021 tcggccggct cgtcgtagaa aacgacggcg ggccgaacgg ccacggcgcg agccagtgca
     4081 acccgtttgc gcatgccacc ggagaggtcg gacggagcca tcgactcaat ccccggcaat
     4141 gcgaccaggg cgagcgtctc ggcaacgcgt tggacgatct cctcttcgct cagcgacgtc
     4201 tgctcgcgca acccatacgc gacgttctcg tacacgtcga gcgagtcgaa aagcgcagat
     4261 tcctgaaaga gcatgccgat ccggcgccgt gtttggttcc attcggcctc ggtctgatgg
     4321 gtcacgtctt cgccgtcgaa tcgaatggtg cccgcatcga cctcgatgag gcccatcatg
     4381 gccttgagca ggatgctctt ccccgagccg gactcgccga tgatcgtgat gacttctcct
     4441 tcgacgatgt cgaggtcgag cccatcgagg accagctgtc gtccaaaggc cttccgaaga
     4501 ccgcgtagct cgaacaatgc catggtctca ggacatcaag ccttcgcgga ccaggagttg
     4561 cagtacccga gaggatgaat cggtcccttg accaaggtgc attggccgca ttcgtccttc
     4621 ttgccggctt tgtaaaacgc gcaggtcgaa cagcgcttct tttcgccgta gggcgagtcg
     4681 tcccggtatt cgagctgggt gcgcaactcc atatcggttt cgctgagccc gctcacgtct
     4741 tggcaatgca gctcgggctt cgtgcaggcg ttgagcagga ccggaaccga gcctacgaca
     4801 accagtcgaa cgctctggcc gagcatctct cgtctcgaaa tggtggtctt tttcatccaa
     4861 tcactccttg gcgaaggcta acacagccgg gcctcgctca gcgaaccgcc ttcatcaagt
     4921 cgagaaacgc ttcgctctcg accaagccgg tgatgcgcga ggcctcggag ccatcgctgt
     4981 tgtgaaggac gactaggggc agaccgggct gattgtaagc tttgagcagc gtccactcgt
     5041 cgtcggtggc ctggtccgtg ctgaggtcga cgcggatcgt cacgaaacgc cgggcttctg
     5101 acacgatccg cgggtcgctc agaacgtcac gttcgagctc ctggcaggcg ccgcaccagt
     5161 ctgccccgaa atcgaccagc atcgggctcc cctccgcgac cgctttcgct ttgccaagat
     5221 cgaagctttc gacccatcga atgtgctcgc cggccggaag ggcctcggcc cagccgacga
     5281 tgccgagcaa gcctgcgacg cacgcgctga ccccgagcgc tttccgaaac ctcgcgcccc
     5341 atgcgccgct ggcgaaggaa agatgaattg ccccggtgat gacgccgccc gcgagaagcc
     5401 cgaacgccac cgccagccac atcgggtcgc gcacggcggg acgcgggtag ggcagcacgc
     5461 cgcgc
//
LOCUS       DASRZF010000377         3538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3722967, whole genome shotgun sequence.
ACCESSION   DASRZF010000377 DASRZF010000000
VERSION     DASRZF010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3722967"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VFG22_16650"
     CDS             complement(<1..366)
                     /locus_tag="VFG22_16650"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HET6417929.1"
                     /translation="MLGKILIYGSYGYTGDLIARFAKEDGVEVVLSGRNSERLRKQAE
                     RYGFQSQPADLSDPKSIRSALKDADVVIHCAGPFAHTYEAMARACLDTGTHYTDITGE
                     AEVYEGLWAMDEQAKRAGIM"
     gene            394..1371
                     /locus_tag="VFG22_16655"
     CDS             394..1371
                     /locus_tag="VFG22_16655"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET6417930.1"
                     /translation="MQINPLQATRHLTGAPRWIWYLLLVKAGQASRTAEYVALFRAIE
                     SSLPADRRLFEDPFARRFLRPKLAMLASLCQVPGVADLVARFVDSRWAGARSSAVART
                     RLIDDTIGRALGHGIEQLVILGAGFDARAYRIGALSDLPVFEVDHPDTLARKRRLLRR
                     VSAEAASHVRFVSIDFRSSGLDQVMTAAGYSKNLRAFILWEGVTNYLNEGAVDATLRW
                     CASAAPGSQVVFTYVHRAVLDDPNSFAGTERLFETLQAAGERWTFGLDPAELAQFLDE
                     RGLLLEEDIGSADYRARYMPRVSQIMRGYEFYRLARACVPGRATEEPIG"
     gene            complement(1368..2852)
                     /locus_tag="VFG22_16660"
     CDS             complement(1368..2852)
                     /locus_tag="VFG22_16660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photolyase"
                     /protein_id="HET6417931.1"
                     /translation="MIPAPNTRIRVLQDGPVRPEGTHVLYWMVGFRRPRWNYALQHAA
                     AHAAKLGRPLLILEALRVGYPWASDRLHRFILDGMRANARHFSERNVVYFPYVERGAG
                     EGKGLLTALSKDACLIVSDDYPTFFVPKMQEAAASRTNLRLELVDSNGLYPMRATDKV
                     FSRAFDFRRHLQKELPKHLGAQPLPDPLEGLAPVSKDDAQTLLASAAPNWKRASESVL
                     RGETLASLPIDHGVAPVELAGGFDAGEARIHDFLASAIDRYGEERSHPDADAASGLSP
                     WLHFGHVSAHQIFDELVKNEGWSSEQLAPKVNGAREGWWGMSSTAESFLDELITWREL
                     GFNMSAHRSDYDQYESLPGWARETLEQHESDERPYLYSLDQLAASETHDPIWNAAQTE
                     LRETGRLQNYLRMLWGKKILEWSPSARDALAVMIELNNKYALDGRDPNSYSGIFWILG
                     RYDRAWGPERPIFGKIRYMSSENTKRKLRMREYLERFGDQGSLL"
     gene            3033..>3538
                     /locus_tag="VFG22_16665"
     CDS             3033..>3538
                     /locus_tag="VFG22_16665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6417932.1"
                     /translation="MSKELRFDGRIAIVTGAGQGLGRSHALALAARGAKVVVNDLGGS
                     TSGEGKSAETADLVVEEIKKAGGEAVANYDSVEDGDSIVRSAMDSWGRVDIVVNNAGI
                     LRDKSFKNMTDADWDIIFRVHNFGAYKVTKAAWPIMTEQGYGRVLFTTSSAGLYGNFG
                     QTNYGSAKL"
BASE COUNT          639 a         1111 c         1100 g          688 t
ORIGIN      
        1 catgatcccc gcacgcttgg cttgctcatc catcgcccac aagccctcat agacctcggc
       61 ttcgccggtg atgtcggtgt agtgcgtacc cgtgtcgagg caggcccgcg ccatcgcttc
      121 gtaggtgtgt gcaaatggtc ccgcgcaatg gatcaccaca tccgcatcct tcaaagcgct
      181 acggatggac ttcgggtccg acaagtcggc aggctgggat tgaaagccgt atcgctccgc
      241 ttgcttccga aggcgctccg aatttcgacc ggataggacg acttccacgc cgtcttcctt
      301 cgcgaagcgt gcgatcaagt cgcccgtgta cccataggac ccgtagatca gaatcttgcc
      361 caacatcgtc aggctccctt gccacctcga acggtgcaga tcaatccgct ccaagcaact
      421 cggcatctga caggcgctcc gcgttggatc tggtatctac tgctcgtgaa agcagggcag
      481 gccagccgaa ccgcagagta cgtggctctg ttcagggcga tcgagtcttc gcttcccgcc
      541 gatcgacggc ttttcgaaga cccctttgcc cggcggttcc tgcgcccgaa gctggcgatg
      601 ctcgcgtctc tctgtcaggt gccgggcgtt gcggacctcg ttgcacgttt cgtcgacagc
      661 cgatgggcag gcgctcgctc atccgccgtc gcgcggacgc gactgatcga cgacacgatt
      721 ggacgagcac tcggacacgg gatcgagcag ctcgtcattc tcggcgcggg attcgacgcg
      781 cgagcctatc ggatcggcgc cctgtcggat ctccccgtgt tcgaggtcga tcatcccgac
      841 acgcttgcaa ggaagagacg attgctgaga cgggtgtccg cggaggctgc gtctcacgtt
      901 cgtttcgtca gcatcgattt cagatcatcg gggctcgacc aggtaatgac cgccgcgggc
      961 tactccaaga acttgcgcgc gttcatccta tgggagggcg tgaccaatta tctgaacgag
     1021 ggcgctgttg atgcgacttt gcgctggtgc gcgtccgccg cacctgggag ccaagtcgtc
     1081 ttcacgtacg tccatcgcgc tgtgctcgac gatccgaatt cgttcgcggg gaccgagcgc
     1141 ctcttcgaaa ctttgcaagc cgctggcgag cgctggacgt tcggcttgga tccggccgag
     1201 ctcgcgcagt tcctcgacga gcgcggcttg ctgctcgaag aggacattgg aagcgcggac
     1261 taccgcgccc ggtacatgcc tcgagtcagt cagatcatgc ggggctacga gttctaccgc
     1321 ctcgcccgcg catgtgtacc gggacgcgct acggaagagc cgatcggcta aagcagggaa
     1381 ccctggtccc cgaagcgctc caggtactcg cgcatccgca gctttcgctt cgtgttctcc
     1441 gagctcatgt aacggatctt gccgaagatc gggcgctctg ggccccaagc tcggtcgtag
     1501 cggccgagga tccagaagat gcccgaatag ctattggggt cacggccatc gagcgcgtac
     1561 ttgttgttga gctcgatcat gacggccagg gcgtcccgcg cgctcggaga ccattcgagg
     1621 atcttcttgc cccagagcat tcggagatag ttttggagac gcccggtctc gcgaagctcg
     1681 gtctgcgcag cgttccaaat tgggtcgtga gtctccgagg ccgcgagctg atccaacgag
     1741 tagagatacg gccgctcgtc gctttcgtgc tgctcgagcg tctcacgcgc ccagccgggc
     1801 agtgactcgt actggtcgta gtcgcttcgg tgcgcggaca tgttgaagcc cagctcccgc
     1861 caggtgatca gctcgtcgag gaacgattcg gcggtcgagc tcatccccca ccagccctcg
     1921 cgcgcaccgt tgaccttggg cgcgagctgc tcgctggacc agccctcgtt ctttacaagc
     1981 tcgtcgaaga tctgatgggc cgagacgtgc ccgaaatgga gccaaggcga tagcccgctc
     2041 gccgcgtccg catcgggatg gctcctctct tcgccgtagc gatcgatcgc cgatgccaga
     2101 aagtcgtgaa tgcgtgcctc gccggcgtca aaaccacccg ccagctccac cggggccacg
     2161 ccgtggtcga tcggaagaga ggctagcgtc tctccgcgga gcaccgattc cgaggctcgc
     2221 ttccagtttg gcgccgctga cgcgagcagt gtctgcgcat cgtccttcga gaccggtgcg
     2281 agcccctcca atggatctgg cagcggttgc gcgccgagat gcttcgggag ctccttttgc
     2341 aggtggcggc ggaaatcaaa cgctcggctg aagaccttgt ctgtcgcccg catcgggtag
     2401 aggccgttgc tgtctacgag ctcgagacgc agattggtgc gtgaggccgc ggcctcttgc
     2461 atcttcggca cgaagaacgt cggatagtcg tcgctcacga tcaggcaggc gtccttgctg
     2521 agcgcggtca gaagtccttt gccttcgcct gcacccctct ccacatacgg aaaatagacg
     2581 acgtttcgct ccgagaagtg ccgcgcgttc gctcgcatcc cgtcgaggat gaaccgatga
     2641 agtcggtcac tcgcccaggg gtagccgacc ctcagagctt cgagaatcag cagcggccta
     2701 ccgagcttcg ccgcgtgcgc agccgcatgc tggagcgcgt agttccagcg gggccttcga
     2761 aatcccacca tccagtaaag gacatgtgtg ccctccggcc gcaccgggcc atcctgtaga
     2821 acgcgaatgc gggtgttggg tgcagggatc acatgtcctc ggatacgttg ccgatgtcta
     2881 tgacccgcgc cgcttccgtc aagaagcccc ggaatcccag gcgccgtcct cggcaaatcg
     2941 ggggcctgca atacatcagg cagttgcggc attgacagcg gcgcgatttc gcataaacag
     3001 tcagctctga ttgtttctga agtcgaggaa gtatgagcaa agaactgagg tttgacgggc
     3061 gaatcgcgat cgtgaccgga gcgggtcagg ggctcggccg ttcccatgcg ctcgcgctgg
     3121 ccgcacgcgg cgccaaggtg gtggtgaatg atttgggggg gtcgacctcc ggcgaaggca
     3181 agtccgcgga aaccgccgat ctggtcgtcg aagagatcaa gaaggccggc ggcgaggcgg
     3241 tcgcgaacta cgactcggtc gaagatggcg actcgatcgt gcggagcgcg atggacagct
     3301 gggggcgcgt cgacatcgtc gtcaacaacg ccggcatcct gcgcgacaag tcgttcaaga
     3361 acatgaccga cgctgactgg gacatcatct tccgcgtcca caacttcggt gcctacaaag
     3421 tgaccaaggc cgcctggccg atcatgacgg agcagggcta cggccgtgtg ctcttcacga
     3481 cctcgtccgc gggcctttac ggaaacttcg gccaaaccaa ttacggatcg gcaaagct
//
LOCUS       DASRZF010000378        42387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_286689, whole genome shotgun sequence.
ACCESSION   DASRZF010000378 DASRZF010000000
VERSION     DASRZF010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 42387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42387
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286689"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..928
                     /locus_tag="VFG22_16670"
     CDS             68..928
                     /locus_tag="VFG22_16670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HET6417933.1"
                     /translation="MKIGIVSINVGGPATAENMIAVAQHAEAAGIESLWTFEHVVVPT
                     YYESKYPYDRSGKMPAAPETWFIDPLISLAYVAAATKTIRLGTGVNIFPQANPMLFAK
                     QTASLDALSGGRLTLGLGIGWLAEEYEAMGTPFERRGARFDDYLEAVKKVWSGEVVEH
                     ESEFLSWHGFKSYPTPAQKPHPPILIGGTTTKTLQRVVRSAQGWYAPSDSTRELSEKI
                     EELKKMAAQAGRPFESIDVTTNWRIAKHPDLLPELEELGIGRAIVLLGATGESDPRKA
                     IDLIASKVGL"
     gene            complement(1029..1466)
                     /gene="trxC"
                     /locus_tag="VFG22_16675"
     CDS             complement(1029..1466)
                     /gene="trxC"
                     /locus_tag="VFG22_16675"
                     /inference="COORDINATES: protein motif:HMM:NF008229.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HET6417934.1"
                     /translation="MGNPSRVLVCPNCQSANRIPSARIGDRPRCGKCKQSLFSGRPIS
                     LTDQSFDRHLSRSEIPLVVDFWAPWCGPCKMMAPFFERAAAELEPHVRLAKVNTDENP
                     MLAQRYQINSIPTTAVFKSGREAARQAGAMNLPQLLQWIRAQV"
     gene            1709..1978
                     /gene="rpsO"
                     /locus_tag="VFG22_16680"
     CDS             1709..1978
                     /gene="rpsO"
                     /locus_tag="VFG22_16680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HET6417935.1"
                     /translation="MALTADRKSELIGQFRNHEKDTGSPEVQIAILSERITYLTEHFQ
                     THKKDHHSRRGLLKLVSQRRRLLDYVRSQDVERYRKIISELGIRK"
     gene            2158..4572
                     /gene="pnp"
                     /locus_tag="VFG22_16685"
     CDS             2158..4572
                     /gene="pnp"
                     /locus_tag="VFG22_16685"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HET6417936.1"
                     /translation="MIIRESVKIGDKEITIETGRIAKQAAGSVLVSSGETMVLVTVCG
                     NKEARPGAPFFPLTVDYVEKTYAAGKIPGGFFKREARQRDHEVLTSRLIDRPCRPLFP
                     DGYMCETQVIATVMSADGSNQSDVLAMVGASAAIHISPLPWAGPIAGVRVARVDGKLI
                     ANPTYQEQENSECELIIAASKDAIVMVEGEASELSEADLIAAIQFGHESVQGVLELIG
                     KIREAVGQQKWPFESPKRDPKIDARVKEVGLAKVIEACNIAEKHPRYGRFSEIKKEVA
                     AELASEFPDLEGDIKAAYEDLRYNTMRAQVLDEKRRVDGRDYKTVRPIAIEVGLLPRV
                     HGSSLFTRGETQGIVTATLGTRQDEQKIDGLIDEYWKPFLLHYNFPPYSVGETKFLRG
                     PGRREIGHGTLAERALTKVLPSREDFPYTMRIVSEITESNGSSSMATVCGGSLSMMDA
                     GVPIKAPVAGVAMGLIMEGEKYAVLTDILGDEDHLGDMDFKVCGTDKGITAIQMDIKI
                     AGLSQEIMHEALEQAREGRLHILEKMNEVMPTHRPELSKYAPRIETLKVKPDQIRVVI
                     GPGGKMIKAITEQTGAKIDISDDGTVSISSPDGEALKKAIEIIESLTLEPEIGTKYKG
                     IVRRVENYGAFLEIAPGKDGLLHITDMDWGFIEKVTDVMDLGDEVEVMISDIDREGRI
                     RLSRKALLEKPEGYVEPERKERREGGGRGRDGDRGRGGRDGDRGRGRGGREGGRDRGG
                     RDRDRGGRGGERAGGREGGRDRDRGRDRDRGGERSGERSGERSGERSGERSSERSSDR
                     DRERKD"
     gene            4572..5018
                     /gene="dut"
                     /locus_tag="VFG22_16690"
     CDS             4572..5018
                     /gene="dut"
                     /locus_tag="VFG22_16690"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HET6417937.1"
                     /translation="MARLRVYKMRADAMIPRYETEGAAGLDLAACLDAPLRLEPGETL
                     RVPTGLRFALPPGHEGQVRPRSGLAARHGVTVLNAPGTIDEDYRGEVQVLLINHGRAA
                     FTIAPGDRIAQLIVAPVSHVEIESVHDEAALGGTKRSDGGFGSTGS"
     gene            complement(5015..5344)
                     /gene="trxA"
                     /locus_tag="VFG22_16695"
     CDS             complement(5015..5344)
                     /gene="trxA"
                     /locus_tag="VFG22_16695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET6417938.1"
                     /translation="MAGANVHSVNDLNFDTEVLSSDSPVLVDFSAVWCGPCKQIAPLI
                     DQLADEYVGKVKVTHLDIDESPGTASKYGIRGVPTLMVFKGGELVAQHIGAAPKTTIA
                     ELMDRAL"
     gene            5496..6296
                     /gene="ccsA"
                     /locus_tag="VFG22_16700"
     CDS             5496..6296
                     /gene="ccsA"
                     /locus_tag="VFG22_16700"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HET6417939.1"
                     /translation="MKAGLFIATAVLYALACILYLLLLTRGAERARRLPGTVFVAALG
                     AHIAFLLLEGPSTHELPLADMSQILSISSFGIGIAFLLASYRFDVTILGAFVAPLSLM
                     LFLASGLGSSYAPVSPPVQSAVLTLHIGANVLGLVAFALAFAAGLAYLVQERLLRRRQ
                     LSGVFQRLPSLDVLDTMSLRAVLVGFPLLTFGMITGTFWLLRSDGSQFFVSQALGLVA
                     WAIFAGVIVLRVAAGWQGRKAALGTMMGFLFTLLVLAGYALRAAAEGG"
     gene            6296..7654
                     /gene="hemA"
                     /locus_tag="VFG22_16705"
     CDS             6296..7654
                     /gene="hemA"
                     /locus_tag="VFG22_16705"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HET6417940.1"
                     /translation="MRDFVLVGLSHRTAPVEVRERLAVPRDRLGEELREIAASGRFDE
                     ALLISTCNRVELYATSSDPIDAAKRARDALAARLLDFAAEDALYQERGVDAVRHVFRV
                     ASSLDSLVVGEPQILGQVKEAFDAAKGAGTVGTLLGRCFTQAFATAKRVRNETGIAQG
                     TVSVSSIACELAKKIFGNLEGRRTLLLGAGEMGESAARSLRQTGTQLHVINRSEDRAH
                     SLAESCGGRAVPYERLTLELAEADVVIASTASPKFILTPEMMKGVVRSRRHRPLFIID
                     IAVPRDVDPRVGNMDNVFVYDVDDLQQVAEENLSVRAREASLAESIVEEELGAFLTWR
                     RSLELAPTIVALRKQFAEVAEQELRRALPRLDGVKDSDRAVLEAMSRSMVNKLLHQPM
                     TQLKAGAGEPDGALLIDAVRRLFALSEEDAGAKEASTEGSGEAKVTQLTTAAAGSSGE
                     ST"
     gene            7651..8631
                     /gene="hemC"
                     /locus_tag="VFG22_16710"
     CDS             7651..8631
                     /gene="hemC"
                     /locus_tag="VFG22_16710"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HET6417941.1"
                     /translation="MKLRIATRGSNLALVQTRWVGAQLRRHFPDLSIEEVHVRTTGDR
                     IQDRPLAQVGGKGLFVSEVEAVVVRGDADFAVHSLKDVPGDVPLADGMGILSVPVRED
                     PRDVLLTIDGVDLASLKRGAKVGTTSRRRVVQLQRQRPDLAFVPLRGNIETRIRKLRQ
                     GQCDAIVLAAAGLSRTGVLDETPHWILPPEICLPAVGQGALAIEGALSNQALREQLLA
                     IEDPTARIQITAERSMLQRLEGNCHSSIAGHAIAADGRLKLEAMVASYDGDQMLCATS
                     DTYLDLNSADAVEGARKLGEEVAEHLLGQGAADLIRQAGVAAMQTQLTSN"
     gene            complement(8613..9002)
                     /locus_tag="VFG22_16715"
     CDS             complement(8613..9002)
                     /locus_tag="VFG22_16715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417942.1"
                     /translation="MYERRTRRSQDPYRALCLQLEQSRSLGDLDAVVVAIDNGLLVAG
                     AGERSICEALGAIAPLLGSPSFNGRMPGALEGQSVDVCPVFLDGETVYVASVGRQGGD
                     PWLSQSINGVRRILGYGPQPESQLLVN"
     gene            9130..10272
                     /locus_tag="VFG22_16720"
     CDS             9130..10272
                     /locus_tag="VFG22_16720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HET6417943.1"
                     /translation="MSLADVLGSHRVVVTVGSGGVGKTTTAAAMAVHAAMEGRTVLCL
                     TIDPARRLANSLGLDEMRTSEQEVPGTLFERNGLECKGALSAMMLDTKRTFDELVEKY
                     ASDEGARDRILNNQIYQYVASSLAGTQEYMAMEKLHEVRKDPKWDLIVLDTPPTSSAL
                     DFLTAPERLIDAIDSPAMRWFLQVFEGAGKEAFGLVGRGATVLLKGIGRITGLEFLEQ
                     VAGFVGGINELFGGFKDRAEQVSNALRSPEVAFVLVTSPDPMAIGEARYFSDTLQASG
                     MNREAIIVNQVHAIIDPPDLTPEEQVRALREVLPPSIDAADLQPRLREALETEHLWAI
                     ADRAEVDRLDEQVGKDTLIVEVPAFDQDVHDLAALARVASYLTGTS"
     gene            10281..12311
                     /gene="glmU"
                     /locus_tag="VFG22_16725"
     CDS             10281..12311
                     /gene="glmU"
                     /locus_tag="VFG22_16725"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HET6417944.1"
                     /translation="MPTDSSTSWSAIILAAGEGTRMKNRRPKVLHEIAGRALVSWVVQ
                     SALDAGVSRCLVVVGHGRNDVERELLGRFGERVEMVLQPERRGTGDAVRCAMEADSRL
                     DGRILILYGDCPLIPPALLRELMKRSDEARADLGLVTATIAEPDGYGRIVRNESGQVQ
                     RIVEHRDCTEDELRIQEVNPGLYTMREDFLRKSITQLTPDNAQGQLYLTDLVELASKH
                     GRVVDLEGDMSNLSGVNDQRDLALAAAAQRQRVAHDLASSGVLVSDLATLYIDAECEV
                     EPGASLGANVHLRGRCLVRTGASIDVGCVLSNVVVEKDARVLPYTVATDSTIGEGAQV
                     GPFSHLRPHTELGPHSKIGNFSETKKTTIGVGSKINHLSYVGDGVIGDGVNIGAGTIF
                     CNYDGAQKHVTVLEDGVFIGSDTQLIAPVTIKKGAYVASGTTVTRDVPEDALAMSRTK
                     QENKEGYAARLRARLRGSKKPSPTGASESDREGSAARGGAEIEVRAFCAPLVILAGTR
                     DAPVGLVSFRRAARCGRCLESEKEWGTDVHSRRETTALALPLSRFDRSAHDAVDEHAR
                     NAQSSSGELLNGGSLLQGFRGIQKLRAARLLGAVIFAPDAFGAPDVWRRDDHMREADE
                     VAARREIRPTELVGLIVVLRRVYGGVGHAAAPSEEKAHPDVAPPGRLLLAVL"
     gene            12710..13762
                     /locus_tag="VFG22_16730"
     CDS             12710..13762
                     /locus_tag="VFG22_16730"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HET6417945.1"
                     /translation="MESVIFKVLITVAALSVLIIIHEGGHYLAARAFGMRVLRYSIGF
                     GPTLFRYQPKGSPTVFQVAVIPFLAYVQIAGMNPNEEVDPNDPELYPNKSLFARISTI
                     AAGPIANYLTASAIVFGLGVTNSLPPMQPAEPMEVGEVVPESPAAVAGLQEGDIIVVA
                     NGQAIKNVSDLIDATTPRAGEPTLYVVEREGQVLPPITITPADRGGRGQIGVSAKMIR
                     LYSNLNAWESAKLSIALPYQMTMAQLNGLAEMVERRSTEGIGGPVMMGKMVADAAEAG
                     LPAFLWILMFISVALGMFNLLPFPALDGGRLIFLGYEAITRRRANERFEMAVHAFGIV
                     LLLGVMILVTYRDIFG"
     gene            13801..14916
                     /gene="thiO"
                     /locus_tag="VFG22_16735"
     CDS             13801..14916
                     /gene="thiO"
                     /locus_tag="VFG22_16735"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HET6417946.1"
                     /translation="MHPDVIVVGGGLMGCSVAYRLAKDGVRVLVLERSIPGAEASSAA
                     AGILGPTVESFDDAFALQLGRRSRELHAELADELDELFGIDVGFRRSGVLKVAFDEEE
                     LAELDTQASKLEIHRVRCDRWSPKELLSEEPAASREALGALSIAEDAQVEPKKLLSAV
                     ALGAEREGATFRSGSTVRAVHTEGGRARGVQLGDELIEADQIVVAAGSWTTLIPGLPF
                     DSDTIYPVRGQMIATHTRPPVFRRIVFGAGGYVVTRPDGRVLCGSTMEHVGFERGITF
                     GGMADVIGKATRLAPGLRSAPIDDHWSSFRPGTSDGLPLVGETRIAGLFLASGHHRNG
                     ILLAPLTAELISDALSGKAPSREAQALSPKRFEESQT"
     gene            14913..15773
                     /locus_tag="VFG22_16740"
     CDS             14913..15773
                     /locus_tag="VFG22_16740"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6417947.1"
                     /translation="MTIGTDEVLVANGLRHHVIRWGDAPVDVVLCHGFLDIGWSFRAV
                     ANELVAAGHGVASFDWRGHGQTEWIGAGGYYHFPDYALDLEDLLPQLSREPVHLVGHS
                     MGGSACVMYAAARPERVRSLTLVEGIGPPEMEPRDPATRLRAWLDGVAKVRGRAARPM
                     SSVESAVARMRIQNPELSDELGAFLVSKSTRPVDGGLQWSFDPLHKTWSPRPFETQSF
                     CRLLEAISAPTLIVAGERGFRLADEEDRVAKIKQKRFVEIADVGHMIHWFKPRVLAEE
                     LADFFEAHAP"
     gene            15807..16751
                     /locus_tag="VFG22_16745"
     CDS             15807..16751
                     /locus_tag="VFG22_16745"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET6417948.1"
                     /translation="MRRIFLLSLVLVGVLAVQALAEDTPPSPDESRRGKVFAKVGTAT
                     ITVGDLEDIINARSPYARMRFVDPEVLRELADEQVRNELFYQGAEKLGYAENPVVRQF
                     VDQTLVQIFVREDFEQAVTPDDVPAEDVAQYYEEHSEEFRRPEMRRARHILVSSEKEA
                     NAILEELRSDADAAFRALAKQKSLDTETNMRGGDLLYFTEDGKLVGKEAGESINPTLA
                     KSAFALASTGDLSKPLDLGDGKWSVLELTGIRPERIQTLEQATGLIRRKLWRAEREGA
                     LDKLMADLRTELKPEVYPERMDAIVLAPSSEPIEPPNQ"
     gene            16838..18016
                     /locus_tag="VFG22_16750"
     CDS             16838..18016
                     /locus_tag="VFG22_16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6417949.1"
                     /translation="MSSTFQPFKEEHRIFRQQVRTFVESELVPNVDEWEEQKLFPNSV
                     FKRAGELGIIGAHYPEDVGGGGGDFWMSVVKSEELARCGSAGVTMGLLVQSDMATPVI
                     NDLGSREVKDEFLAPAISGDKVAALGVSEPGAGSDVAGLRTTARAVGDDYVINGSKTY
                     ITNGTRADFVTLMVKTDPDAGHSGISIIVCPTDVKGFSVSKKLEKAGNWSSDTAELFF
                     EDVRVPKRYLLGQEGMGFVYLMQNFQSERLVGCVSGLEGSKLALDRSVQFGRERVAFG
                     KPLIKREYWQQKFVDLYTKYEAAKALSYNACAAYNEEKFVNGGMLSMETTRIVSMAKA
                     YVGDVTAEIMDQCLQFHGGMGYLEELWVARAWRDARLFRIGGGATEVMRYAIAKMMGF
                     "
     gene            18109..18639
                     /locus_tag="VFG22_16755"
     CDS             18109..18639
                     /locus_tag="VFG22_16755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417950.1"
                     /translation="MKSVFMVALMVALMLVGCGDEAPPWSPGGITNIPGTITGGGGTS
                     GTGGMGGVGGSGGGASGACDNPSDLDAIEGAAPSIRDIARNCGLTSCALFVLNGPAYQ
                     ICVDACVESSVEGLSTPCARCYGALERCGRDAICFESCSLNTCSSFCTNCLAAAGCIT
                     EFEECRGLPGDDCPPP"
     gene            complement(18689..19720)
                     /locus_tag="VFG22_16760"
     CDS             complement(18689..19720)
                     /locus_tag="VFG22_16760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417951.1"
                     /translation="MTRLARREPLLVRRGARFDCVGDGLCCSDIHAVGALSDEDCEML
                     ASISEDAFDRHEGEDAAVLMMRSDTGRCVFWSDQGCAIHSQLGPQMKPTPCIQFPYAL
                     TATPQGGRISTQHRCTCRTLGSLPPVSVELARPCLLDSNGELKPEHAVLDEVAWSPSE
                     LISFAEYAEREARMIDALLAGKGLSTVLSAQPFPELDSMTWAEVADDLLDFGGPSRAA
                     FASRWFGDAIGFLVERRERTESGRPWADSFERAAERVVDPVPPNQVFGDWLADEVWSM
                     RWTRFGTFARARTELATRLAIARRIAGWLDISSPKSDNISAAEAVMIVDVIGTSDPWE
                     RVQEAIAQP"
     gene            complement(19717..21216)
                     /locus_tag="VFG22_16765"
     CDS             complement(19717..21216)
                     /locus_tag="VFG22_16765"
                     /inference="COORDINATES: protein motif:HMM:NF012730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HET6417952.1"
                     /translation="MLARDYLHGAFHDPRTQAYRVVESFVWILILLSITVLIAEPFFA
                     EGSAGDRVLQQIDRLLLWLFAIEVSTRILTYRPPELAVFNKPPLGRLRTEIFSRIRFA
                     LTPMMLIDILTVLALVPALRGLRALRLLRLLRTQRLFRYANPFRGLMHAFEENRLLFA
                     FAFAVLGVETILGGISLFLVERNVNPEITSVGDGLWWALVTITTVGFGDITPVSTLGR
                     VIGGVMMVGGLFTLALFAGIIGHSLLHAVLSIREEQFRMSGYVNHIVVCGYEMGSGML
                     LDVLSSEIDTEEQRVVLFGPYERPRELSPEFLWVQGDPTKESELDKVRITHASTVIVT
                     GSRRIKPQEADAATILTVFTIRSALTQSPAAQSRKIPVRIIAEVLDSENVQHARTAGS
                     DEVVETRRVGYSLLAHTVVYPGVADATSRMVFAGNQNLYVGRVPKGTEMPVRFDRLSG
                     QIRATTGCMVIGIRDPKTGKEQVNPGSDVIVTPGMEILYLGPAPRLESA"
     gene            complement(21277..23508)
                     /locus_tag="VFG22_16770"
     CDS             complement(21277..23508)
                     /locus_tag="VFG22_16770"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HET6417953.1"
                     /translation="MGPIWMTLLLVSTLGFFSWSAFRRIRQVKIGVPDPRFAWTGEQI
                     ADRAKTLLVYAFGQKKMPNYTLAGFAHVGIFVAFQVLLLNSLMLWGRGFDPGFDFWGL
                     LSTDHLVGKLYSFVKELAAFGAIAGSVVFLYLRWVKRGEDSGDPKAVKDKPRMTLGHH
                     ANRYIFTEPILILFIIITMMAADFLYVGSSLVLDARAYGEPGQGIHAGWWEPFGGIFA
                     QLLSGVDSTRIVTAFQHAGFWWHSAFVLIFLNILPYSKHFHVLTVIPNVFAYERRPNA
                     LPKVEDLEGKVEREESLGINRLTDLTYKHIMDLYTCTECGRCSDNCPAYITGKKLSPK
                     HLTLAIRDHLYATEKDMFGKAALVAEPSDKPVEPPKPSDGEQPIHTFPAAPEGGYFVG
                     ETVVDLVPNILHPDVIWGCTSCRACEEQCPVMISYVDKIIGMRREEVMIKNEFPGELQ
                     GAFNGIETNGNPWNISSMDRGDWAHGLGIPLLSDKPDAEVLYWVGCAASYDDRAKKVA
                     RAVSKLLKKARVDFAILGTEETCTGDPARRAGNEYLFQMLAEQNIETLNGYEASKKTI
                     ITACPHCFNILGNEYGDFGGTYDVVHHSDFLNGLLVAGKLVPSKAVKGKVVYHDSCYL
                     GRYNQVYDSPRQILEAIEGLELTEVPYWNKKKGLCCGAGGAQMFMEEQGEERVNNKRT
                     LQLLDTGATTIASGCPFCMTMLTDGLKKQDKDEEIGQRDIAEILADAIELDEVEQTPV
                     AAE"
     gene            23577..25199
                     /locus_tag="VFG22_16775"
     CDS             23577..25199
                     /locus_tag="VFG22_16775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417954.1"
                     /translation="MRRIEVKRRVLVAQDWEGPFHYDPRVRRLGSIFLLGVAACASPA
                     MRTVPRVVDGEVENGPFVSPYAYEWFIEGEALAAKGQHAQAAIAFENAAAAPDDDVLL
                     MTRLAEEYELSGAYRRADRTLSLARRNDPSSARVALAEGRILQSRGAEAEALSSFARA
                     MDLAPAWEEPVIEMAVSLNALGHPQRANAVLLDYVENSIASRSASARKFLLELAREAK
                     DGRLYERALLLDSSSTAESRAEAAGALSLEAGRPAMAARILGRALDSTKNTNLWIRAL
                     SQSGDRARALDFLAESQSDQVEGDVWRAEWLVRLGDAQRALDSLQAMSGSPRVELSRG
                     EALAARGEYASAAAVLSRIPFGASTFEASRLALVECSTSLGRAGAATEALSQAPHGSL
                     SVRAKVAEFYLEEGDLPAALRLFDPKNPKERALIARLFEEAGEFREAAAYYASLKPSF
                     TDGSPLAARASAERLAAHGHLGRAVSVLEGWRTAAPDDLYARVRLVELLLAQSRRNDA
                     MREGRDALGLIEDQMLSAHLSDLLTAQSNLSR"
     gene            complement(25207..25815)
                     /locus_tag="VFG22_16780"
     CDS             complement(25207..25815)
                     /locus_tag="VFG22_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6417955.1"
                     /translation="MSKALPPVRVLVVDDDQAILEFMETFLTKDGFEVTTLANPKDAP
                     NEVKEGGYHLVVLDLMMPGLGGVELLEQIRRVDSDVAVVIFTGYPSLDTAVQSMKLDA
                     VDYLKKPFNPDEFREVLDRVMRKKGLLRTPEENLHRVIGETIRGLRKDRALTLKQMAR
                     RTGLSVSLLSQIERAESSASISSLYKVATALDVRIQDLFGEY"
     gene            complement(25857..25930)
                     /locus_tag="VFG22_16785"
     tRNA            complement(25857..25930)
                     /locus_tag="VFG22_16785"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25894..25896),aa:Pro,seq:cgg)
     gene            complement(25981..27306)
                     /locus_tag="VFG22_16790"
     CDS             complement(25981..27306)
                     /locus_tag="VFG22_16790"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HET6417956.1"
                     /translation="MVAAARRIATRVLYRVVHDDAWAAPTLDAELRRESVGRSDAALA
                     TQIVYGTLRTIPALDASLARHARRLGKLDQWTHIALLSAVYQLHHLGRIPSHAVVNDT
                     VELVRAKRGKGLAGFVNAVLRKVAADRPAVPAPPPAVVVPDWLRDALTASIGTEHGAD
                     LLRIGDEPPSIDLRVRLDQDRDLIAEAILAAHPDAAVSRTSISPLGLRISGVGDPRAL
                     PGYSEGAFAVQEEGAQLIGLLLEAKPGERVLDACAGRGGKTAQLLEAVGESGRVIATD
                     FHEHRLEQIPAELQRLRLNTARLETACVDWTVGAGGVQGGFDRVLVDAPCTGLGTLRR
                     RPEILLRAGTDDAARMGETQRLVLENAAALVRPGGTLLYAVCSPLKEEGSALLERVLL
                     RGFELQVGRASGLKSLYFGSLGQLAIGPWVEGAGPWADAYQVYMWVNVG"
     gene            27426..28298
                     /locus_tag="VFG22_16795"
     CDS             27426..28298
                     /locus_tag="VFG22_16795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6417957.1"
                     /translation="MSALVLVADPDPFNLRLLHEACEVAGYDVVTAADGPEVLAMIAR
                     RPPDLLILDAKMSEIDAFEILRILKADAGLLDIPVLLVTDESDIDARRRGIELGAEDY
                     LTRPYRVFEIQRRVRNVLRATRRDRIGIPESTPPADQRDPVTGAGTPSQLLISLDYEH
                     TRAERYGHPLSCIVVKLGNSNEIAAASGDGTADQAMARIGTGLKACIRAVDHLFRLRR
                     DEFCILLPETDGDGAGYVLRRLEGQVESGKLWGEDVVPRPNIQIGLTSTPEERVKSGD
                     DLLQQTRRSLAKKS"
     gene            complement(28295..28750)
                     /locus_tag="VFG22_16800"
     CDS             complement(28295..28750)
                     /locus_tag="VFG22_16800"
                     /inference="COORDINATES: protein motif:HMM:NF022281.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HET6417958.1"
                     /translation="MSRTMMTSPDRTRANPHWQRLGWWALALGSVLVLLAAAWLEPDS
                     RGHGTHTQLGLPPCGFLLLTGAPCPGCGLTTAFAHGIRGSWVAAASANPLGLALFFAV
                     CACIPIGIVAGWRGWSLDAVIERFALNRWALALGAGAVALWVLRFASAL"
     gene            complement(28719..29270)
                     /locus_tag="VFG22_16805"
     CDS             complement(28719..29270)
                     /locus_tag="VFG22_16805"
                     /inference="COORDINATES: protein motif:HMM:NF016314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HET6417959.1"
                     /translation="MSIDEPDREILALQIGRPLRATSTVLKRCSLGLPVVADVPPLLD
                     TGEPFPTLYWLTCPLAHRRIARIEAEGGVRAEQARIDANPSLSEALKVRHDGYARARD
                     ARLPADAPLRPSGGVGGSSGGVKCLHAHYADFAAGNDNPVGRHVHESIGEPDCALPCV
                     VIIDGQPKRNPDWREPNHDDEPR"
     gene            complement(29267..30265)
                     /locus_tag="VFG22_16810"
     CDS             complement(29267..30265)
                     /locus_tag="VFG22_16810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417960.1"
                     /translation="MLPRPGARYTCFGDGLCCTDIHGIGPLTKKEVAEMRRIERKSAG
                     WDDDHDDYMLKTAADGGCVFLMSDQRCSVHAKLGPEFKPEGCRRFPLGLVATPVGGRI
                     TTEHRCPCRTMGERPLIEPEDVESSIANGGGQLVADRRIKRIPMTAKKEIEFSEWESI
                     EVEYLARLRGREPLLRILDAEPFPRLKGSSWDKQADEFIGARDGSQFGVAMAWVGDMI
                     SAIQNRRSPRPPPRPWAAAFDRAQARSPKPRTSRDVFGDWIADEIWSLKWAEDHNFAV
                     ARAELATRLTIAERISALLRDGGARADRASAEAVMMLEVVGESDFWTEIEDRMRIS"
     gene            30375..30905
                     /locus_tag="VFG22_16815"
     CDS             30375..30905
                     /locus_tag="VFG22_16815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417961.1"
                     /translation="MGASPSPLVGYNTNVRHKGKLYHIQTEDSGVKRPHVITQLFADG
                     GRIIASEKTSYAEHVESEDLTAIVKQLMQEQHKRVFIALRDGAYDEDGPSEARSDGQS
                     ATTSDLEVTTEVLEHAAARLVEGAPHYEQVRAGSVSQQRISRAPAAPFGHDVLSEKTL
                     DEVILSYLAEDLETGT"
     gene            30984..31307
                     /locus_tag="VFG22_16820"
     CDS             30984..31307
                     /locus_tag="VFG22_16820"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HET6417962.1"
                     /translation="MPDAKISFTENIPKSIAKDLEKARAVDAVFLFRVTGEEGGTWTV
                     NMKDEPGVTEGDAGNAECTIELSNEDWRAMTEKPGTAMQFFMHGKIKVRGNALLATKL
                     QAVIG"
     gene            31322..31597
                     /locus_tag="VFG22_16825"
     CDS             31322..31597
                     /locus_tag="VFG22_16825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417963.1"
                     /translation="MPIIKERIFMLLQDPRVMKLMRDPKVQAIVMKGFRYRGRVEGAF
                     DQGVQRIAGKLNLATQRDLRGLQRRIRHLERELREAEERLNEDQAAR"
     gene            complement(31572..32069)
                     /locus_tag="VFG22_16830"
     CDS             complement(31572..32069)
                     /locus_tag="VFG22_16830"
                     /inference="COORDINATES: protein motif:HMM:NF024822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HET6417964.1"
                     /translation="MVDPDRSISALRRWAERLVTLLDDRFRIPGTDFRFGLDPVIGIL
                     FPGIGDAVTGVGSVGLLTLALRRGVPRVVLMRMILNILVDVVLGALPIIGDIFDAAYK
                     SNRRNLELIREHESDASKPSRWDYAVVTLGVILAILSIIVPLVAMFYFGAYYGPALIE
                     RLGLR"
     gene            complement(32091..33407)
                     /locus_tag="VFG22_16835"
     CDS             complement(32091..33407)
                     /locus_tag="VFG22_16835"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6417965.1"
                     /translation="MRDLGVNAAAASEPVTDGENRIFATGELLDGVYEIMQLLGRGGM
                     GQVFEAHDHLLNRRVAIKAAWPNPLTPPLRNEARALAAFQHPSLVSVHTLGEHRGIDY
                     LVMERVYGVSLTQHASTRWGSGEQFTPAEVVQLLLPAAEGLSVVHRAGLVHRDIKPDN
                     IMLTPAHRVVLMDFGLVLPEFDVNGKQRVAGSPPYMAPEALLNTAATGSGHLVDIFGL
                     GVVAYELLTGGRPYPGTTIREVIASHEHAQLAPLAEARIDCPLALCQVVHEMLSPNPQ
                     VRIQSAEAVAWQLRAILDERPRSGGVARSGRSGPSVLIVDDDADLARILTFYVRQIIG
                     SSEIRVAYDGEEALSEVQKEEPDVMLVDLHMPRMNGVELCMQLKGEGLAQGCSIISVS
                     AGAQEHDVQLLHQLGIHHFVEKGSNLRERLRSVFVEVCGDHVLADA"
     gene            complement(33452..34258)
                     /gene="murI"
                     /locus_tag="VFG22_16840"
     CDS             complement(33452..34258)
                     /gene="murI"
                     /locus_tag="VFG22_16840"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HET6417966.1"
                     /translation="MAKPIGIFDSGLGGLTVARAIAGALPRENLVYLGDTARVPYGTR
                     SAQTVVKYAIGCAERLLSFDIKLLVVACNTVSGVALGELQSRLDIPVIGVIEPGARAG
                     VRATRTGRIGVLATSGTVASGAYERAIHAENEAMEVIATAAPLFVPLAEEGWVSGEVP
                     RLAARRYLQPLADADVDTIVLGCTHYPLLSGTIHEVAAELLGHELAIVDSADATAATL
                     GEILSPEPATPGPPGSLEILVTDLPGQFGAAASRFLGKAVEGLDIRQIDL"
     gene            34338..35726
                     /locus_tag="VFG22_16845"
     CDS             34338..35726
                     /locus_tag="VFG22_16845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET6417967.1"
                     /translation="MPALALISRDPTLYQELVAALDGTAWSVEWLGAEVLDGGELHQE
                     VVLLDATHPGAETWSTRGSKPVLLLVDGTGTQALEERADDIIFRPPGRRELEARLERL
                     HARTPLDELRRQYAKTFASMAPGIVVIDPDLQMVFANPRSYEIVGHREETIAASGIAR
                     VVAPKDLPRVRDALSQGQYPRGMDIHLIRRDGTQITCSTSFAPLIGAEDHTVISFEDV
                     TDIRATERELLKTMEFLESLIEASVDAIIATDMEQRVILFNPSAERVSGRHVSDVVGK
                     VKLEDLLGRASSELVTQRLLASDHGGEGRLEPTMLDLLDIHGARIPVQLSASLIYEHG
                     EPSAIVLIFADLRDRIRVEERLAEAQKKLAFTEKQGVLAELAGTAAHELNQPLTSMMA
                     YAELLLRQSEEGSSGAKAARVLIREAERMAEIVRKIGKITRYETTSYVGHQKIFDLDR
                     AVDTSSDPRTKG"
     gene            35895..37418
                     /locus_tag="VFG22_16850"
     CDS             35895..37418
                     /locus_tag="VFG22_16850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6417968.1"
                     /translation="MTDEKILRAYVHALERLYPNIRFAMRLSPRNLERASIIQATTHR
                     VSPDAMDVLSITEAGLRRAGCADTLEPPSEIELRRTYEPILLAGPDEESLPADGFDAP
                     LLRNDIVFGVLAVEFERGTDLPSRLEDAVLMTATQVSAAIEIARLRRESLHLRDYLEK
                     LLEHANIPVLVIGRDRSIRVVSGAFGRITGLDRADLVAKDVLQLAGQSERVRVLSAFV
                     SALRGRDVPPFELRLPKAGGGNARLQFTLAPIVDSEGRVAGVIAIGRDRTEVRELEGQ
                     VIHAEKLATLGQLAAGIVHEINNPLTSISVYGEYLLSKLSRTGAEQSDLNRVERILRS
                     SDRIMSFTRNLLTYARPSKEEPQPVSLNDVVEEAVVFCEHIVREAGVEVSCDYGEQLP
                     AVNAVAGQLHQVFVNLITNACNAAPEEGGFVRLATHLHGHDRIHVEVHDNGVGIQPSE
                     LSRVFEPFFSTRRKGKGTGLGLSIVKNIIEEHRGSIEIQSEVGKGTTVLVTLPCLDI"
     gene            37524..38915
                     /locus_tag="VFG22_16855"
     CDS             37524..38915
                     /locus_tag="VFG22_16855"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HET6417969.1"
                     /translation="MFGTTFRRPWVIALAATSAIAIAGGALASPKKQLIEDDVLERAL
                     AFETALTSVAEAVSPSVVSIQVEVTRPEANGIPFFFGGQSGSGIVRGGGSGVIVRSDG
                     YILTNNHVVNGANRIDVRLQNGKSFQAKLVGADSATDLAILKIDARGLPEATFASSED
                     ARVGQLVIAIGSPFGLDYTVTTGVLSAKGRDGIGANEIEDYLQTDASINPGNSGGPLV
                     DLRGSVLGINTMIVGRNSGIGFAIPSEIAQRVASQLIQNGSVKRAWLGISFQEITPEL
                     AAHFGGDFEGGALINAVVPDGPAEKAGLQAGDVITAIGDQRIQEGHELLRTVLRQGVG
                     ERVVLEVQRGGKTSKVALVTGARPSGNEPQSLQQGTEDDGVLGLALEDLTPNLRKRFG
                     YEGDGRVFVRGVKPGSDADRAGLRPGDIILQADREPVRSVADVRTALKDGQALLYIER
                     ESRRFFQPIARSK"
     gene            complement(38918..40492)
                     /locus_tag="VFG22_16860"
     CDS             complement(38918..40492)
                     /locus_tag="VFG22_16860"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6417970.1"
                     /translation="MNTSAGYYQAPRQQRVPLDEVLIKLHPEGFHQEFEADGINMGAG
                     GLSMRASVVPEVGSSLRCQLDNPTGGEAIEMIGRVVWAHESGPHVGEFGIRFTHLSND
                     DQSRIEALIQRWGRTTAKAPIVQLQLDGVGSPIMAEIRSETRTALSVEQPLPFLAIGS
                     TVLNQSAGRRGHLEAVELKMDEKTPKLVLSIGYEVDPAMGVSEEGADRDTVPEDMPAA
                     AQVAPRPREERIHDAPRIVRYDAAADEPGSEPALDRLRAYAKSAADQLASSVGGARMA
                     VTQGLIPRAASSAKEMFGVAGRFIAALRSRLDDKPRRKQVPRSEAARAHLKNTRRDRR
                     RYLIVAAMACGIMAAGWGIYLWAAASSGTDNEAVTEATTEVDELPVEATKSVKGTGAS
                     SGTKTPTKTESAAPIEATQLQAQGAPLPPKSVRGIAFGADAVPGGEKYVLRMSNPVTL
                     MDGIIETDGFSVTIPSSLSLSRAGPIASAHPDVEDASILNRGDFSELTVRFVAGRHPA
                     YRVEARGPAIEITIAR"
     gene            40625..41281
                     /locus_tag="VFG22_16865"
     CDS             40625..41281
                     /locus_tag="VFG22_16865"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET6417971.1"
                     /translation="MGVRITVQSLWTGADASALVYEFTQSRIVIGRSRSADVQLPHAA
                     VSGSHAIIREQDAGYVLVDEGSTNGTRINEVLVVPGRPKTLRSRDLVDLGGYRLSIDV
                     CVPVSQPMSARLTADHAKRILSEQLGSTNADEIDAELRAIQSGPDEHVELLPITGERV
                     SEAPLPRESRPSKPRESRPESGPPPEPVRLARSELAVYALASVVITICIVAMALLMKH
                     "
     gene            complement(41278..42042)
                     /locus_tag="VFG22_16870"
     CDS             complement(41278..42042)
                     /locus_tag="VFG22_16870"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HET6417972.1"
                     /translation="MLLVVDVGNTHTVLGLYDGERLVHDFRIETAKGRTSDEYHVLLL
                     NLLSLAGVDRSDVRASILASVVPSFSDTVIEAVDRAFDHEIMVVGPGIKTGMPVLYEN
                     PREVGADRIVNAVAAFERVKGAAIVVDFGTATTFDCVSEKGEYLGGAIAPGMQISANA
                     LFSRAARLPRSEIARPPRAIGRNTVHSMQAGIVFGYVGLVDGLVRRLAAEMESEVAVI
                     GTGGLASLIEPDSETIDEIDEFLTLEGLRLIYLRNA"
     gene            complement(42043..>42387)
                     /locus_tag="VFG22_16875"
     CDS             complement(42043..>42387)
                     /locus_tag="VFG22_16875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417973.1"
                     /translation="GAQATPFPSSFPTQLPSSPVLEPPTSPVMDSPTRYSSQPPRVER
                     PLSIEPRARSIRPSSAAPAAAHSSDRPQAIRPQSVAPTGQPVVYATDPPEFPRAKTFG
                     DLLDLTLSLRPR"
BASE COUNT         8298 a        13091 c        13287 g         7711 t
ORIGIN      
        1 cgcggagtga agccgatctc ggaatcggcc tgcgccctcc acaagcgatc atgataagaa
       61 ggtcgggatg aagatcggga ttgtatcaat caacgtcggc ggaccagcga cagccgagaa
      121 catgattgcg gtggcccagc acgccgaggc tgctgggatc gagtcgctgt ggaccttcga
      181 gcacgtggtc gttccgacct attacgaatc gaagtatcct tatgaccgct cgggcaagat
      241 gccggctgca cccgaaactt ggttcatcga cccgttgatc tcgctcgcct acgtggccgc
      301 cgcgacgaag accattcggc tcggcacggg cgtcaacatc ttcccgcagg ccaatccgat
      361 gctgttcgca aagcaaaccg cgtcgctcga tgcgctgagc ggcggtcgcc tgacgctcgg
      421 tctcgggatt ggctggcttg ccgaggagta cgaagcgatg ggcacgcctt tcgagcgacg
      481 cggcgcacgc ttcgacgact acctcgaggc ggtgaagaag gtctggagcg gtgaagtcgt
      541 cgaacacgaa agcgagttcc tctcgtggca tggcttcaag agctacccca caccggcaca
      601 gaaaccgcat ccaccgattc tcatcggcgg aacgacgacc aagaccttgc aacgcgtcgt
      661 ccgctcggct cagggctggt acgcaccgag cgattcgacg cgggagctca gcgagaagat
      721 cgaggagctc aaaaaaatgg ccgcccaagc gggacggcca ttcgagtcga tcgacgtcac
      781 gacgaactgg cgaatcgcga agcatcccga cctactgccc gagctggaag agctcggcat
      841 cggccgggcc atcgtgctac tcggcgcgac aggcgagtcc gatcccagga aggcgatcga
      901 cctgatcgcc tccaaggtcg gcttgtgatc agatgcggtg ccttacggcg tgaagaggca
      961 agtcgcccag gcactgatct ctgactcgca gaacacctca ccgagacccg tggtcccatc
     1021 cgtgacggtt agacttgggc tcgaatccac tggaggagct gcgggaggtt catcgcgcct
     1081 gcttgccttg cggcctcgcg accgctcttg aagaccgcgg tcgtcggaat actgttgatt
     1141 tggtagcgct gggcgagcat cgggttttcg tcggtattga ctttggccag tcgcacatgc
     1201 ggctcgagct ctgctgcggc tcgttcgaag aagggcgcca tcatcttgca ggggccgcac
     1261 caaggagccc agaagtccac gacgaggggg atctcgctcc ggctcaagtg tcggtcgaag
     1321 ctttggtcgg tgagcgagat cgggcgcccg ctgaacaagg actgcttgca cttcccgcag
     1381 cggggtctat cgccgatccg ggcgctgggg atgcggttgg cggactggca attgggacaa
     1441 acgaggactc gagacgggtt gcccattgcg tgtcaaggta agtccgaaat caggcttggc
     1501 tagggtatca tgaaggattc aagagcaatg agcagcagcg ccgagggctg cgtttccact
     1561 gcgtctggtc ggggagctga cagcaccgct gcgcctccgt cggagcctgg aaaacggttc
     1621 cttgacgcta cctggacccc ggcccatatt ccgccccctt caggcaaaga aatacccatt
     1681 ttttcaagag aattagcgcg agtaacgcat ggcactgacc gccgatagaa agtccgagct
     1741 gatcggtcaa ttccgcaacc acgagaagga cacgggttcc cccgaagtcc agatcgcgat
     1801 tttgagcgag cggatcacct acctgaccga gcacttccag acccacaaga aggaccatca
     1861 ctcccgtcga ggcctgctca agctggttag ccagcgacgc cggctgctcg actacgtccg
     1921 atcccaggac gtggagcgat accgcaagat catttcagag cttggcattc ggaagtaagc
     1981 tgagccccgg gacgttgtcc cagtagaacc ttggtggctt tgcgtcgggt ccggagttcg
     2041 ctcttcgctt cgatgatttg agttcgggct caaatcctgg aatcggggac cgagaccgct
     2101 ctcgatggca acgcggaccg catgcgcggt tgtgccgcgc ggaaggcaaa gagagcgatg
     2161 ataatcagag aatccgttaa gatcggtgac aaagagatca ccatcgagac gggccggatt
     2221 gccaagcagg cagccggctc cgtattggtg agcagtggcg agaccatggt gctcgtcaca
     2281 gtttgtggaa acaaagaagc gcggccagga gcgccgttct ttccactcac cgtcgactac
     2341 gtcgagaaga cgtatgccgc aggcaagatt cctggcggct tcttcaagcg cgaggcgagg
     2401 cagcgcgatc acgaggtgct cacctctcgt ctgatcgacc gaccctgccg gccgctcttc
     2461 cccgatggct acatgtgcga aacccaggtc atcgcgaccg tgatgagcgc agacggcagc
     2521 aatcagtccg acgtcctggc gatggtcggt gcctcggccg cgattcacat cagcccgctt
     2581 ccttgggcag gcccgatcgc tggcgtacgt gttgcccgcg ttgacggaaa gctgatcgca
     2641 aaccccacct atcaagagca agagaacagc gaatgcgagc tgatcatcgc cgcgtccaag
     2701 gacgcgatcg tgatggtcga aggcgaagct agcgagttgt ccgaggctga cctgatcgcc
     2761 gccatccagt tcggccacga gtcggttcag ggtgtgctcg agctcatcgg aaagatccgc
     2821 gaagcggtgg ggcagcagaa gtggcccttc gagtcgccca aacgtgaccc gaagatcgac
     2881 gcgagggtca aagaggtcgg tctggcgaag gtcatcgagg catgcaacat cgccgagaaa
     2941 cacccgcgct acggccgctt cagcgaaatc aagaaggaag tcgctgccga gctagcttcg
     3001 gaatttccag acctcgaagg cgacatcaag gccgcgtacg aagaccttcg ttacaacacg
     3061 atgcgggctc aggttctcga cgagaagcgc cgcgtcgatg gtcgtgacta caaaaccgtt
     3121 cgcccgatcg ccatcgaggt gggtctgctg cctcgcgtcc atggttcctc gctgttcact
     3181 cgaggcgaga cccagggcat cgtcacggcg accctcggca cccgtcagga cgagcagaag
     3241 atcgacggtc tcatcgacga gtactggaag ccgttccttc tccactacaa cttcccgccc
     3301 tactcggtcg gtgagaccaa gttcctccgc ggcccaggcc gtcgggagat cgggcatgga
     3361 accctcgccg agcgtgcgct cacgaaggtg ctgccctcgc gtgaggactt cccttacacg
     3421 atgcgcatcg tctcggagat caccgagtcg aacggctcgt catcgatggc gaccgtgtgc
     3481 ggcggaagct tgtcgatgat ggacgcaggt gtgccgatca aggctcccgt cgcgggtgtg
     3541 gcgatgggcc tcatcatgga gggcgagaag tacgcggtcc tcacggacat cctcggcgat
     3601 gaagaccacc tcggcgacat ggacttcaaa gtctgcggca ccgacaaggg catcacggcc
     3661 attcagatgg acatcaagat cgccggcttg agccaggaga tcatgcacga agcgctcgag
     3721 caggcgcgcg agggccggct tcacatcctc gagaagatga acgaagtcat gccgacgcat
     3781 cgccccgagc tctcgaagta cgctccgcgc atcgagactt tgaaggtcaa gcccgaccag
     3841 attcgcgtgg tcatcggacc aggcggcaag atgatcaaag cgattacgga gcagaccggc
     3901 gccaagatcg acatcagcga cgacggtacg gtgagcatct cgtcgccaga cggcgaggcg
     3961 ctcaagaagg caatcgagat catcgaaagc ctcacgctcg agccggagat cggcacgaag
     4021 tacaagggca tcgttcggcg cgtcgagaac tatggcgcgt tcctggagat cgctcctgga
     4081 aaggacgggc tgctccacat caccgacatg gactggggct tcatcgagaa ggtcacggac
     4141 gtcatggacc tcggcgacga ggtcgaggtg atgatcagcg atatcgatcg tgaaggacgc
     4201 atccggcttt cgcgcaaggc gctcctcgag aaacccgagg gctatgtgga gcctgagcgc
     4261 aaagaacgcc gcgagggtgg cgggcgcggg cgcgatggcg atcgaggccg cggaggacgt
     4321 gacggcgacc gaggacgtgg gcgcggcgga cgcgagggcg gcagagaccg tggcggacgc
     4381 gatcgggacc gtggtggccg tggcggcgaa agagccggtg gacgcgaggg cggcagggat
     4441 cgcgaccgcg gtcgtgaccg tgaccgcggg ggcgagcgca gtggcgagcg cagcggcgag
     4501 cgcagtggcg agcgcagcgg cgagcgcagc agcgagcgca gcagtgaccg cgatcgcgag
     4561 cgaaaagact gatggcgcgt ctgcgcgtct acaagatgcg cgccgacgca atgattccgc
     4621 ggtacgagac cgaaggcgcg gcggggctgg acctcgccgc gtgcctcgat gcgccgttgc
     4681 ggctcgagcc gggagaaacc ctgcgtgtgc cgacggggct acgattcgcg ctgccgcccg
     4741 gacatgaggg ccaggtccgc ccgcgctccg gccttgccgc acgccatggc gtgacggtgc
     4801 tcaatgcgcc gggaacgatc gacgaggact accgcgggga ggtgcaggtt ctcctcatca
     4861 atcacgggcg cgcggccttc acgatcgcgc caggtgaccg catcgcgcag ctcatcgtcg
     4921 cgccggtcag ccatgtcgag attgaatcgg tccacgacga agccgcgctc ggtggaacca
     4981 agcgcagcga cggtgggttt ggaagcaccg gctcctagag cgcgcggtcc atcagctccg
     5041 cgatggtggt cttgggcgct gcgccgatgt gctgggccac gagctcgccg cctttgaaga
     5101 ccatcaaggt cggcacgcct cgaatcccat acttcgaggc cgtcccgggg ctctcgtcga
     5161 tgtcgaggtg cgtcaccttg accttgccga cgtactcatc cgcaagctga tcgatgagcg
     5221 gtgcaatttg cttacaaggc ccgcaccaaa ccgccgagaa atcgacgagc acgggggagt
     5281 ccgagctcag aacctccgtg tcaaagttca ggtcgttcac tgaatgtacg tttgcgccag
     5341 ccatctcagc tcctattcgg attgttggag atgctgtaag taaggacggt ccgacgaatt
     5401 gtcaacgtgc cgcgcccgcc ggctaccgtg caataccccc acatggtagg ctttttcgcg
     5461 gccggaccca ccctgcggga ggccacgaac agaccatgaa agccggcctc ttcatcgcca
     5521 ccgcagttct gtatgccctc gcctgcattc tgtatctatt gctcctgaca cgcggcgcag
     5581 agagggcgcg cagactacca ggaaccgtct tcgttgcagc gctgggcgcg cacatcgcgt
     5641 tcctgctgct ggagggcccg tcgacgcacg agctgcccct ggccgacatg tctcagatct
     5701 tgagcatttc gtcttttgga atcgggatcg cgttcctgct ggcgtcgtat cgtttcgacg
     5761 tcaccatcct cggcgcattc gtagcgccgc tctccctgat gctcttcctc gcgtccgggc
     5821 tcggcagcag ctatgccccg gtctcgcccc ccgttcaatc cgcagtgctc actcttcaca
     5881 tcggtgcgaa cgtcctcggg ctggttgcat tcgcgctggc atttgcggca gggcttgcgt
     5941 acctggttca agagcgcttg ctgcgccggc gccaactgag cggcgtgttt caacgccttc
     6001 cttcgctcga cgttctcgat acgatgagtc ttcgtgcagt cttggttggg ttcccgctcc
     6061 tcacgttcgg aatgatcacg ggcacgttct ggctgttgcg ttccgacggt tcgcaattct
     6121 tcgtgtccca ggcgctcggc ctggttgcat gggcgatctt tgcgggcgtg atcgtccttc
     6181 gagtggcggc gggctggcag ggtcgaaaag ccgccctcgg cacgatgatg ggcttcctct
     6241 tcacactcct ggtcctcgcg ggctacgcgc tgcgcgctgc tgcggagggt ggctgatgcg
     6301 cgatttcgtg ctcgtcggcc tgtcccatcg aacggcaccg gtagaggtcc gggaacgcct
     6361 cgccgtgcct cgggaccgac tcggcgaaga gcttcgcgag atcgccgcca gtggacgttt
     6421 cgacgaggcg ctcttgattt ccacctgcaa tcgtgtcgag ctctacgcga ccagcagcga
     6481 cccgatcgac gcggcgaaaa gagcgagaga cgctcttgcg gcccggctcc tcgatttcgc
     6541 cgccgaggac gcgctctatc aggagcgcgg cgtcgatgca gtgcgccacg tgttccgcgt
     6601 cgcttcgagc ttggactccc tcgtggtcgg agagcctcaa attctcggcc aggtcaaaga
     6661 ggcgttcgac gcggcgaagg gcgctgggac ggtgggcacc ttgcttgggc ggtgcttcac
     6721 tcaagccttc gcgaccgcaa agcgcgtccg aaacgaaacc ggaatagcgc agggaaccgt
     6781 cagcgtgagc tcgattgcct gtgagcttgc gaagaagatc ttcggcaacc tcgaggggcg
     6841 tcgcactttg ctgcttgggg ccggcgaaat gggcgaatcg gcggcacgaa gccttcgaca
     6901 aacgggaacc cagctccacg tcatcaatcg aagcgaggat cgcgctcatt cactagcgga
     6961 atcctgcggc ggacgtgcgg tgccctacga gcgactcacg ctcgagctcg ccgaggcgga
     7021 cgtcgtcatc gcatcgacag caagcccgaa gttcattttg acccccgaaa tgatgaaagg
     7081 cgtagttcgc tcacgacgtc atcgcccgct tttcatcatc gacatcgccg tcccacgtga
     7141 cgtggatccg agggtcggca acatggacaa cgtcttcgtc tacgacgtcg acgacctcca
     7201 acaagtcgcc gaggaaaatc tttccgtgcg cgccagggaa gcctctttgg cagaaagcat
     7261 cgtcgaagag gaactcggtg cctttctgac ctggcgacgt tctctcgagc tcgccccaac
     7321 catcgtcgct cttcgaaaac aattcgccga agtggccgag caagaattgc gacgtgccct
     7381 gccccgcctc gacggggtca aagacagcga tcgagcggtg ctcgaagcga tgtcgcggtc
     7441 gatggtgaac aagttgctgc accaacccat gacgcagctc aaggcgggcg ctggcgagcc
     7501 ggatggcgct ctcttgatcg acgccgtccg tcgtctgttc gcgctctccg aggaagacgc
     7561 tggggccaaa gaagcctcga cggaagggtc cggcgaggcc aaggtcacgc agttgacgac
     7621 cgcagcagcc ggctcgtcgg gcgaatcaac gtgaaactac gcattgcaac acgcgggagt
     7681 aacctcgctc tcgttcaaac gcgctgggtg ggcgcccaac tccggcgaca ctttcccgat
     7741 ctctcgatcg aggaagtcca cgttcgaacg acgggcgacc gtatccaaga tcgcccactt
     7801 gcccaggttg gcggcaaggg actcttcgtg agcgaggtcg aggcggtggt cgtacgcggt
     7861 gacgccgact ttgcggtcca ctcgctcaaa gacgtgcccg gggacgtgcc cctggcggac
     7921 ggcatgggca tcctcagcgt gcccgtgcgc gaggatcctc gcgacgtcct gctgaccatc
     7981 gacggcgtcg atttggcttc cctcaagcga ggcgcgaagg tcgggaccac ttcgcgccgc
     8041 cgcgtcgtgc aacttcagcg gcagcgtccg gacctcgcat tcgtcccgct tcgcgggaac
     8101 atcgaaacgc gcattcgcaa gctccgccaa ggacaatgtg acgcgattgt cttggcagcc
     8161 gcagggctca gtcggacggg cgtgctcgat gaaaccccgc attggatatt gcctccagag
     8221 atttgcctgc cagcagtcgg acagggtgcg ctcgcaattg aaggcgcgct gagcaaccag
     8281 gctctgcgcg agcagctcct ggccatcgaa gaccctacgg cacgcattca gattacggcc
     8341 gaacgcagca tgctccaaag gctcgaagga aattgtcatt cttcgatcgc aggccatgcg
     8401 atcgccgcag acggaaggct caagctcgaa gccatggtcg catcgtacga cggcgaccaa
     8461 atgctttgtg cgacttcgga cacctatctc gacctcaact cagccgacgc cgtcgagggc
     8521 gcacgaaagc tgggcgagga ggtcgccgag catctcctcg ggcaaggagc cgccgacctg
     8581 atccgtcagg caggcgtggc cgccatgcag actcagttga ccagcaactg agactcgggc
     8641 tggggcccgt agccgaggat ccggcgaacc ccgttgatcg actggctgag ccaagggtca
     8701 ccgccctgtc ggccgacgct tgcgacgtac accgtttcgc catcgagaaa cacggggcag
     8761 acgtccacgc tctgcccttc gagcgccccc ggcattcgtc cattgaagct tggggagccc
     8821 aggagtggag cgatcgcacc gagtgcttcg caaatggagc gctcgcccgc gccggcgacc
     8881 agcaaaccat tgtcgatcgc aacgacgact gcgtccaagt cgcccagcga gcgcgactgt
     8941 tcgagctgaa ggcaaagcgc cctatatgga tcctggcttc ggcgggtgcg gcgttcgtac
     9001 atgaggactc cctggcagat gtaggtgcat gtagactatt ggcctacgag acgaggggca
     9061 agattccgtc gaaaccattg atttcagcga aatcgacacc gcgtacaaga ctgctagagt
     9121 cgcgctgcaa tgagtctcgc ggacgtgctt ggcagtcacc gcgtcgtggt gaccgtcggc
     9181 agcggcggag tcggcaaaac cacgacagcg gcagcaatgg ccgtgcacgc ggccatggaa
     9241 ggccggacgg tcctatgcct caccatcgac cctgcgcgac gcctagccaa tagcctcggg
     9301 ctcgatgaga tgaggaccag cgagcaggag gttccgggca cgctcttcga gcgcaacggc
     9361 ctcgaatgca aaggcgcgct ctccgcgatg atgctcgaca cgaagcggac tttcgatgag
     9421 ctggtggaga aatacgcttc cgatgaaggg gcgcgtgacc gcatcttgaa caaccagatc
     9481 taccagtacg tcgcgagctc gctggcggga acgcaagagt acatggcgat ggagaagctc
     9541 cacgaggtgc gcaaggatcc gaagtgggac ctgatcgtgc tcgacacccc gcccacttcg
     9601 agcgccctcg actttctgac tgcgcccgag cgactgatcg acgcgatcga ctctcctgcg
     9661 atgcgctggt ttctgcaagt gttcgaaggc gccggcaaag aggctttcgg cttggtggga
     9721 cgcggcgcga ccgtgcttct caaaggcatc ggaaggatta cgggcctcga attcttggaa
     9781 caggtcgcag gattcgtggg tgggatcaac gagctcttcg gcggcttcaa ggatcgcgcc
     9841 gagcaggtgt caaacgcgct tcgaagccct gaagtggcgt tcgtgctcgt gaccagcccc
     9901 gatccgatgg cgatcggcga agcgcgttac ttcagcgaca ccttgcaagc gtccggcatg
     9961 aaccgcgagg cgatcatcgt caatcaggtg catgcgatca tcgacccgcc cgacctcaca
    10021 cccgaagaac aggtgcgggc gctgcgcgag gtcctgcctc cgtcgatcga cgccgccgat
    10081 cttcagccac gtcttcgcga agccctggag accgagcatc tgtgggcgat tgccgaccga
    10141 gccgaggtcg accgattgga cgagcaagtg ggaaaagata cccttatcgt ggaagtgccc
    10201 gcattcgatc aagacgtcca cgacctcgcg gcactcgcaa gagtggcttc ctatctgacg
    10261 gggaccagct gatcgcaacc atgccaaccg actcatccac tagctggagc gccatcatcc
    10321 tcgctgccgg agaaggcacg cgcatgaaga accgccgccc caaggtcctc cacgaaatcg
    10381 caggacgtgc gctcgtttcc tgggtcgtgc agagtgctct cgatgccggc gtatctcgct
    10441 gcctcgtggt ggtgggtcac ggccgcaacg acgtcgagcg cgagctgctg ggccggtttg
    10501 gcgagcgggt tgagatggtg ctgcagccgg agcgtcgcgg gactggcgac gcagtgcgct
    10561 gcgcgatgga agctgactcg cgcctcgacg ggcgaatcct gatcctctac ggcgactgcc
    10621 ccctgatccc gccagcgcta cttcgagaac tgatgaaacg gtccgatgaa gcgcgggccg
    10681 acctcggatt ggtcaccgcc acgatcgccg agccggatgg ctacggccga atcgtccgca
    10741 atgaatcggg ccaagtgcag cggatcgtgg agcatcggga ctgcaccgaa gacgagttgc
    10801 gcatccagga ggtcaatcct gggctctaca cgatgcgcga agatttcctg cgcaaatcca
    10861 tcacgcagct gacccccgac aacgcccagg gccaactcta cttgacggat ctcgtcgagc
    10921 tggcctccaa gcacggtcgg gtggtggatc tggagggcga catgtccaat ctcagcggag
    10981 tcaatgacca acgggatctc gcgctcgccg ccgccgcgca gaggcaacgg gtcgctcacg
    11041 atttggcaag cagtggcgtg ctcgtttccg acctggcaac gctgtacatc gacgctgaat
    11101 gtgaagtcga acctggcgca tcgctggggg ccaatgtcca ccttcggggg cgctgcctag
    11161 tccggaccgg cgcaagcatc gacgtgggtt gcgttctcag caacgtcgtc gtcgagaagg
    11221 atgcgagagt tctgccctat acggtcgcga ccgactccac gatcggagaa ggcgcgcagg
    11281 tcggtccatt cagccactta cgcccccaca cggagctcgg tccacacagc aagatcggaa
    11341 acttctcaga gaccaagaag acgacgatcg gcgttggctc gaagatcaac catctttcct
    11401 acgttgggga cggcgtgatc ggggatggcg tgaacatcgg tgctgggacg atcttctgca
    11461 actacgacgg ggctcagaag cacgtcacgg tgctcgagga cggcgtcttc atcgggagcg
    11521 acacccaact catcgcaccc gtgaccatca agaagggggc atacgtggcg agcggaacga
    11581 ccgtcactcg cgacgttccc gaagatgcgc tggcgatgtc gcgaaccaag caagaaaaca
    11641 aggagggcta tgcagcccgc cttcgggcgc gcctccgcgg gagcaagaag ccctcgccga
    11701 ccggcgcctc agaaagtgat cgtgaaggtt ccgccgcccg gggtggcgct gaaatcgaag
    11761 tccgggcctt ctgcgctccg ctcgtaatac ttgctggaac acgcgatgcc ccggtaggcc
    11821 tcgtaagctt tagacgcgcc gcgcggtgcg gtcgctgtct ggagagcgag aaggagtggg
    11881 ggaccgacgt acattcccgc cgtgagacca cggctctggc ccttccactg agtcgctttg
    11941 accgttccgc ccacgacgcc gtagatgagc atgcccgcaa cgcccagagc tcctccggag
    12001 agctcctgaa cggaggcagc cttctccaag gtttccgtgg catacagaag cttcgcgcgg
    12061 cgcgcctcct cggtgctgtc atctttgcgc ctgatgcgtt tggcgcccca gacgtctggc
    12121 gcagggatga tcatatgcgt gaggccgacg aagtagctgc ccgccgcgag atacgcccaa
    12181 ccgaacttgt cggcctgatc gttgtcctgc gccgcgtgta cggcggcgta ggtcatgccg
    12241 ccgcccccag cgaggaaaag gctcatccag acgtagcgcc accgggtcgc ttgcttcttg
    12301 ccgtcctgta gcttcccttc gatgtacttg atgcgatact gcacctcgtc atcgctcagc
    12361 tcctcgatac agctataggc atcttgggct gtggccgctc gaggcggagc cagaatcgtg
    12421 aaagcaagcg cgcaggtcag gaccagcgtg atgcggtgga tgagtgagtt cgcgtttcga
    12481 tgatgttcag gcacaaccta cccctgttcg ccaggtggcc gggatagtac gcaaaacctc
    12541 cccaaactgc gatagtcatt cgcataatca gtcacttttt ttcggacctt cgaattcccc
    12601 gcaaatgtat ggatccggcg ggaactagat actgtcgggg gtgaaacgcg gggttcgggc
    12661 tctatcgcag ctgcctcgac gggccggatc ggggcgttat gctgggggcg tggaaagcgt
    12721 gattttcaag gttctcatca cagtcgcggc gctgtcggtg ttgatcatca ttcatgaggg
    12781 tggacactat ttggcggccc gagcgttcgg gatgagggtc ttgcgataca gcatcgggtt
    12841 tggcccgacg ttgtttcgct accagccaaa aggcagccca accgtcttcc aggtcgcggt
    12901 gatcccgttc cttgcgtacg tgcaaatcgc cggaatgaat ccaaacgagg aagtcgaccc
    12961 aaacgatccg gagctctacc cgaacaagag cctgttcgcg cggatctcga caatcgccgc
    13021 gggaccgatc gcgaattatc tcaccgcatc cgcgatcgtt ttcgggctgg gagtgaccaa
    13081 cagtctgcct ccgatgcaac ctgcggagcc aatggaggtc ggtgaggtgg tgcctgagtc
    13141 gcctgcggcg gtggccggtt tgcaggaagg cgacatcatc gtcgtggcga acggccaagc
    13201 gatcaagaac gtttccgatc tcatcgatgc gaccacgccg cgtgccggcg agccgacact
    13261 ctacgtggtg gagcgcgaag gccaggtcct gccgcccatc acgataaccc ctgcagaccg
    13321 aggcggacgc ggccagatcg gcgtcagcgc caagatgatt cggctctatt cgaacctcaa
    13381 cgcctgggaa tccgccaagc tctcgatcgc gcttccctat cagatgacga tggctcagct
    13441 caatgggctc gccgagatgg tggaacggcg gagcaccgag ggcatcggcg gcccggtgat
    13501 gatgggcaag atggtcgccg acgctgctga ggccggattg ccagcctttc tgtggatatt
    13561 gatgttcatc tcggtggccc tcgggatgtt caatttgctg ccgtttcctg cgctcgacgg
    13621 gggtcgactc atcttcctcg gatacgaggc gattacgcgc cgacgagcca acgagcggtt
    13681 cgagatggcc gtccacgcat tcggaatcgt gctgctattg ggggtcatga tcctcgtcac
    13741 gtatcgcgac attttcggat aggcgcttgc cgcgcaaagc ggagcgtgta gcttccggaa
    13801 atgcatcccg atgtcatcgt agtcggaggc ggcttgatgg gttgcagcgt cgcctaccgc
    13861 ctcgccaaag acggggttcg cgtcctcgtt ctcgaacgca gcatccctgg agccgaagcg
    13921 tcgtctgcgg cggcggggat cctcggaccc accgtcgagt cattcgacga cgcatttgcc
    13981 cttcagctcg gccggcgcag tcgtgagctc catgccgagc tcgctgacga gctcgacgag
    14041 cttttcggga tcgacgtcgg atttcggcgc tctggggttc tcaaggtcgc gttcgatgag
    14101 gaggagcttg cggagctcga tacgcaagcc tccaaactcg aaatacaccg cgtccgttgc
    14161 gatcgctgga gccccaaaga gctgctcagc gaagaacctg ccgccagtcg ggaagcactc
    14221 ggagcactca gcatcgctga agatgcgcag gtggagccta agaagttgtt gtcggccgtt
    14281 gctctcgggg cggagcgcga aggcgcgacc tttcgcagcg gatccacggt ccgcgcggtg
    14341 catacggaag gcggccgggc ccgcggcgtg caactcggcg acgagctcat cgaggcagat
    14401 cagattgtcg tcgctgcggg cagttggacc actctgattc cgggtctccc attcgattcc
    14461 gatacgatct atccggtgcg cggccagatg atcgcaaccc acacgcgtcc ccccgtcttc
    14521 aggcgaatcg tttttggcgc tggcggctac gtggtcacgc gcccagacgg ccgcgtcctt
    14581 tgcggctcta cgatggaaca cgtcggtttc gagcgcggaa tcactttcgg agggatggcc
    14641 gacgtcatcg gcaaggccac gcgtcttgcg cccggtctcc gatcggctcc catcgacgac
    14701 cactggtcga gctttcgccc cggtacgtcg gacggcctac cgctggtcgg cgagacgcgg
    14761 atcgccggat tgttccttgc cagcgggcat catcgtaatg gaatcctgct cgcaccgctg
    14821 accgccgagc tcatctcgga cgccttgtcc gggaaagcgc catcgcgcga ggcgcaggca
    14881 ctctccccga agcgcttcga ggaatcgcaa acatgaccat cggcacggac gaagttctcg
    14941 ttgccaacgg ccttcgacat cacgtgatcc gctggggcga cgctccggtc gacgtagtcc
    15001 tttgccatgg ctttctggac atcggttgga gctttcgcgc cgtcgcaaac gagctcgtcg
    15061 ccgcaggaca cggggtcgct tcgttcgact ggcgcggtca cgggcagacc gaatggattg
    15121 gagccggcgg ctactatcac tttccggact acgcactcga tctggaggat ttgctccctc
    15181 agctgagtcg agagcccgtc catctggtcg ggcactccat gggcggttcg gcgtgcgtca
    15241 tgtacgccgc agcacgcccc gagagagtgc gaagcttgac gttggtcgaa ggtatcggcc
    15301 ccccggagat ggagccgcgc gaccctgcga cacgcttgcg cgcttggctc gacggggtcg
    15361 caaaggtccg gggacgcgcc gctcgcccga tgagcagcgt cgagagcgct gtggcccgga
    15421 tgcgtatcca aaaccccgag ctgagcgatg agctcggtgc gtttctggtc agcaagagca
    15481 cgagaccggt ggatggcggg cttcaatgga gtttcgatcc actgcacaag acctggtccc
    15541 cgcggccctt cgagacccaa tcgttttgca ggcttctcga agcgatctcg gcccctaccc
    15601 tcatcgtcgc cggagaacgc ggcttccgat tggcggacga agaggaccgg gtcgcgaaga
    15661 tcaaacagaa gcgcttcgtc gaaatcgccg atgtcgggca catgattcac tggttcaaac
    15721 cgcgtgtgct cgctgaagag ctcgctgact ttttcgaggc gcatgcgcct tgaccgggtg
    15781 atagcctcgt gaaacgctgc ctttccatgc gacggatttt tttgctttcc ctcgtgctgg
    15841 tcggtgtcct ggcggttcaa gcgcttgccg aagacacgcc cccgagtccc gatgaaagcc
    15901 gccgcgggaa ggtctttgca aaggtaggca ctgcgacgat caccgtgggc gatctcgaag
    15961 acatcatcaa cgcgcgctct ccatatgccc ggatgaggtt tgtcgacccc gaggtgctca
    16021 gagagctcgc agacgagcag gttcgtaacg agcttttcta ccagggcgcc gagaagctcg
    16081 gctatgcgga gaatcccgtg gtccggcagt tcgtcgatca gacgctcgtt cagatattcg
    16141 tccgcgagga tttcgagcaa gccgtcacac cggacgacgt cccggcggaa gacgtcgcgc
    16201 agtactacga ggagcactcg gaagagtttc gtcggcctga gatgcgccgc gcaaggcaca
    16261 tcctcgtaag cagtgaaaag gaagccaacg ccatcctcga agagctgaga tctgacgcgg
    16321 acgcagcctt tcgcgccctc gcgaagcaaa agagcctcga caccgagacg aacatgcgag
    16381 gtggcgacct gctttacttc acggaggacg gaaaactcgt cggcaaagag gcaggcgagt
    16441 ccatcaatcc cacgctcgcg aaaagcgcgt ttgcgctcgc gagcacgggt gacttgagca
    16501 aaccgctcga cctcggcgat gggaagtgga gcgtgctcga gctcacgggg attcgaccag
    16561 agcgcatcca gactttggag caggcgaccg gcctcattcg tcgcaagcta tggcgagcgg
    16621 agcgcgaagg cgctctcgac aagctgatgg ccgacctgcg caccgagctg aagccggagg
    16681 tctatcccga gcggatggat gcgatcgttc tggcgcccag cagcgagccg atcgagcccc
    16741 cgaaccaata gaacacgcgg gcgctgctat gggcttgcca attcgctgcg ctccggctac
    16801 taaatgaacg ttcattcatt ttcggagcca gggagctatg tcatctacat tccagccctt
    16861 taaggaagag catcggatct tccgtcagca agttcggaca ttcgtcgaaa gcgagctcgt
    16921 gcccaacgtc gacgagtggg aggagcagaa gctgttcccc aactcggtgt tcaagcgagc
    16981 cggtgagctc ggcatcatcg gagcgcacta ccccgaggac gtgggcggcg gcggcggcga
    17041 tttctggatg agcgtggtca agtcggagga gctcgcgcgc tgcggttctg caggtgtgac
    17101 catgggcttg ttggttcagt cagacatggc cacacccgtg atcaacgatc tcggctcgcg
    17161 tgaagtcaag gacgagttct tggcgcccgc catcagcgga gacaaggtcg ccgcgctcgg
    17221 cgtgagcgag ccgggcgcag gctcggacgt tgcaggcctg cggaccaccg cccgagcggt
    17281 cggtgacgac tacgtgatca acggctcaaa gacatacatc accaatggca ctcgcgccga
    17341 cttcgtcact ctgatggtga agaccgatcc ggacgcgggc cacagcggta tttcaatcat
    17401 agtctgtccg accgatgtca aaggcttcag cgtttcgaag aaattggaga aagccggcaa
    17461 ctggtcgagc gacacggcag agcttttctt cgaggacgta cgagttccca aacgctacct
    17521 tctcggacaa gaaggaatgg gattcgtcta tctcatgcag aacttccagt cggagcgact
    17581 ggtcggctgc gtaagcgggc tcgaaggatc caagttggct ctggaccgtt ccgtgcagtt
    17641 tgggcgcgag cgcgtcgcgt tcggaaagcc cttgatcaag agagagtact ggcagcagaa
    17701 gttcgtcgac ctctatacca agtacgaagc cgcaaaggcg ctctcgtaca acgcatgcgc
    17761 cgcgtacaac gaagaaaagt tcgtgaacgg cggtatgttg tcgatggaga cgacgcgcat
    17821 cgtctcgatg gccaaggcct acgtcgggga cgtgacggcc gagatcatgg atcaatgcct
    17881 gcagttccat ggcggcatgg gatacctcga agagctctgg gtcgcgcgcg cgtggcgtga
    17941 tgcccggctc ttccggattg gcggcggcgc gacggaagtg atgcgctacg cgatcgcgaa
    18001 gatgatgggc ttctgagcga agggtcgccg ccgcgaccga ttgctcagag agcgatatcc
    18061 tcaagccgtt cgttgaccta aatcgcaaat cgggacagac ttggcgccgt gaagtcggtt
    18121 tttatggtcg cgctcatggt cgcgctcatg ctcgtcggtt gcggcgacga ggctccgccg
    18181 tggagtccgg gcggcatcac taacattccg ggtacgatca cgggcggggg cgggaccagc
    18241 ggaaccggag gcatgggcgg cgtgggcggc tcaggcggcg gcgcgagcgg cgcctgcgac
    18301 aacccgtcgg atctcgacgc gatcgaagga gcagcgccaa gcatccgtga catcgcgcgc
    18361 aactgcggcc tgacaagctg cgcgctcttc gtgttgaacg ggcccgcgta ccagatctgc
    18421 gtcgacgcgt gcgtcgaatc cagcgttgag ggtctctcca ccccatgcgc gcgttgctac
    18481 ggggccttgg aacgctgcgg gagagatgca atctgcttcg aatcgtgctc gctcaacacc
    18541 tgcagctcgt tttgcaccaa ctgcctggca gccgccggct gcatcacgga attcgaggag
    18601 tgtcgaggtc ttcccggaga cgactgccct cccccgtagc cacggcggat ctgcctgcaa
    18661 ctgacccagg tgctggcacc gacctggtct agggctgcgc aatcgcttcc tgcacccgtt
    18721 cccatggatc cgaggtgccg atgacatcga cgatcatcac tgcttccgcc gcggagatgt
    18781 tgtcgctctt cggcgagctg atgtcgagcc agcccgcgat tcggcgagcg atggcgaggc
    18841 gcgtcgcgag ctcggtgcgt gcacgagcga aagtgccaaa gcgcgtccag cgcatcgacc
    18901 agacttcgtc ggcaagccag tctccgaata cctgattggg tggaacggga tctacgacgc
    18961 gctcggccgc gcgttcgaac gagtcggccc acgggcgccc gctctcagtc cgctctcgac
    19021 gctcaaccag aaagccaatg gcgtctccga accagcgcga agcaaaggca gcacgcgagg
    19081 gccctccgaa atcgagcaga tcgtcggcta cttcggccca ggtcatcgag tcgagctccg
    19141 ggaacggttg cgcgctaagc acggtgctga ggcccttccc tgccaggagc gcatcgatca
    19201 ttctcgcctc gcgctcggcg tactcggcga acgaaatcag ctcgctcggg gaccaagcca
    19261 cttcatcgag caccgcatgc tcaggcttca gctcgccatt cgaatccaaa aggcaaggcc
    19321 gcgccagctc cacactaacc gggggaagag aaccgagggt gcggcaggtg cagcggtgtt
    19381 gagtcgagat tcgaccacct tggggtgtgg cagtgagggc gtacggaaac tgaatgcaag
    19441 gcgttggctt catctgggga ccgagctgtg agtgaattgc acatccttga tcgctccaaa
    19501 acacacatcg gccggtatcg ctccgcatca tgaggaccgc cgcatcctcg ccctcgtgcc
    19561 ggtcgaaggc atcttcggat atcgaagcga gcatttcgca gtcttcatcg ctcagcgcac
    19621 ccaccgcgtg gatgtcgctg cagcagagtc catcgcccac acaatcgaat cgtgcgcctc
    19681 ggcgaaccag aagcggctcc cgcctggcga gtcgggtcac gcgctctcca atcgcggcgc
    19741 agggccgagg tagaggatct ccattccagg ggtgacgatc acgtcacttc ccgggttgac
    19801 ctgctctttg cctgtcttcg ggtcgcgaat cccgatcacc atacaacccg tggttgcacg
    19861 aatctgtcca ctcagccggt cgaatctaac gggcatctcc gttcctttcg ggacgcggcc
    19921 cacgtagaga ttctggttcc ccgcgaacac catccggctc gtcgcgtcag cgacgcccgg
    19981 gtagacgacc gtgtgtgcaa gtagggagta ccccacgcgg cgagtttcga cgacctcatc
    20041 gctgcccgca gtccgagcgt gctgaacatt ctcggaatcg agaacctccg caatgatgcg
    20101 cacgggtatt ttccgggatt gggcagccgg actttgggtg agggcagaac gaatcgtgaa
    20161 caccgtgagg atggttgccg cgtctgcttc ctgaggcttg attcttctgg agccggtgac
    20221 gatgaccgtc gacgcatgcg tgatgcgcac cttgtcgagc tcactttcct tcgtcggatc
    20281 gccctgcacc cagaggaact ccggactgag ctctctcggt cgttcgtagg gcccgaacaa
    20341 gacgacccgt tgctcttccg tgtcgatctc cgagctcagc acgtcgagca gcattccact
    20401 gcccatctcg tagccacaaa ccacgatgtg attgacgtaa ccgctcattc gaaactgctc
    20461 ctcccggatg ctcaagaccg cgtgtaacag gctgtgaccg atgatgcccg cgaaaagggc
    20521 caaggtgaac aagccgccga ccatcatcac gccgccgatg actcttccca gggtcgagac
    20581 cggagtgatg tcaccgaagc caacggtggt gatcgtgacc agagcccacc ataaaccatc
    20641 gccgacacta gtaatctcgg ggttgacgtt acgttcgacg agaaagaggc tgatgcctcc
    20701 gaggatcgtc tcgacgccga ggacggcgaa cgcgaaagca aacagcaatc ggttttcctc
    20761 gaacgcatgc atgaggccac gaaatggatt cgcataccga aacaacctct gcgttctgag
    20821 cagacgaaga aggcgcaaag cgcgcaagcc acggagcgcg ggtaccagcg ccaagaccgt
    20881 gaggatgtcg atgagcatca tcggagtcag cgcaaatcgg atccgagaga agatctccgt
    20941 gcgcaacctc ccgagtggtg gcttgttgaa aaccgcgagc tcgggcggcc gatacgtcag
    21001 gatgcgcgtg ctcacctcaa tcgcgaagag ccacagcagg agccgatcga tttgttggag
    21061 gactcggtcg ccggccgatc cttccgcgaa gaaaggctcc gcgatcaaca ccgtgatcga
    21121 caagaggatc aggatccaaa cgaacgattc gacgacgcgg tacgcttgtg tgcgcggatc
    21181 gtgaaatgca ccgtgcaagt agtcgcgcgc gagcatgcgc cgcgagcatc aatcgacgag
    21241 gattgggtgt caaccgccct cctctggcag cttgggctat tcggcagcga caggcgtctg
    21301 ttcgacctcg tcgagctcga tcgcatccgc aaggatttcg gcgatgtcac gttggccgat
    21361 ctcttcgtcc ttgtcctgct tcttgaggcc gtcggtcaac atggtcatgc agaacggaca
    21421 tccactggcg atcgtcgtcg cacccgtatc gagtagctga agcgttctct tgttgttgac
    21481 tcgctcctcg ccctgctcct ccatgaacat ctgcgcgccg ccagcaccgc agcaaagacc
    21541 cttcttcttg ttccagtacg gaacctcggt cagctcgagc ccctcgatcg cctccaggat
    21601 ctggcggggc gagtcataga cctggttgta gcggccgagg tagcagctgt cgtggtagac
    21661 gaccttcccc ttcaccgctt tggaaggaac gagcttgccc gctaccagca ggccgttgag
    21721 gaagtcgctg tggtgaacca cgtcgtacgt cccgccaaag tcgccatact cgtttccgag
    21781 aatgttgaag cagtgcggac aggccgtgat gatggtcttc ttggaggcct cgtaaccgtt
    21841 gagtgtttcg atgttctgct ccgccagcat ctggaaaagg tactcgttgc ctgcacggcg
    21901 cgccgggtcg ccggtgcagg tctcttcggt tcctagaata gcgaagtcga cgcgagcctt
    21961 cttcaagagc ttagacaccg cgcgtgcgac cttcttggct cgatcgtcgt agctggcagc
    22021 gcatccgacc cagtacagaa cttcggcgtc aggcttgtcg ctcaagaggg ggatgccgag
    22081 cccatgcgcc cagtcacctc ggtccatcga agagatgttc caggggttgc cgttggtttc
    22141 gatgccgttg aacgcaccct ggagctcgcc ggggaactcg ttcttgatca tgacttcctc
    22201 gcgacgcatg ccgatgatct tgtcgacgta tgaaatcatg acgggacact gctcttcaca
    22261 ggcgcggcac gaggtgcagc cccaaatcac gtccggatgc agaatgttcg gtaccagatc
    22321 cacgaccgtt tcaccgacga agtagcctcc ctcgggggca gcggggaacg tgtgaatcgg
    22381 ctgttctccg tcgctcggtt ttggcggctc caccggcttg tcgctcggct ccgcgactag
    22441 agcggccttt ccgaacatat ccttctcggt cgcgtacaga tgatctcgaa tcgccaaggt
    22501 gaggtgctta ggcgagagtt tcttcccggt gatgtaagcc gggcagttgt cgctgcaacg
    22561 cccgcactcg gtgcaggtgt acaggtccat gatgtgcttg tacgtgaggt cggtgagtcg
    22621 gttgattccg agggactcct cccgctcgac cttgccctcg aggtcctcca ccttgggaag
    22681 cgcgtttggg cgacgctcgt aagcgaagac gttcggaatg acggtcagga cgtggaagtg
    22741 cttcgagtac ggcaagatgt tcaagaagat caacacgaaa gccgagtgcc accagaaacc
    22801 cgcatgctga aacgcggtga cgattctcgt gctgtccacg ccggatagaa gctgcgcaaa
    22861 gattcccccg aacggttccc accagccggc atgaatgcct tggccaggct cgccgtaggc
    22921 gcgcgcatcc aataccagcg aagaccctac gtagagaaag tcggcagcca tcatcgtgat
    22981 gatgatgaag agaatgagaa tcggctccgt gaagatgtag cggtttgcgt ggtggccgag
    23041 tgtcatgcgg ggcttatctt tgaccgcctt cgggtcgccg ctgtcctctc cacgcttcac
    23101 ccatcggagg tacaggaaca cgacggagcc tgcgattgcg ccgaaggcgg cgagctcttt
    23161 cacgaaagag tacaacttgc cgaccaagtg atcggtgctg agcaggcccc agaaatcgaa
    23221 cccggggtcg aagccgcgtc cccagagcat gaggctgttg aggagcagca cctggaacgc
    23281 aacgaagatg ccgacgtgcg cgaatccagc caacgtatag ttgggcatct tcttctgccc
    23341 gaaggcatag acgaggaggg tcttcgctct gtcggcgatc tgttcacccg tccaggcaaa
    23401 ccgtgggtcg ggcaccccga ttttgacctg gcgaatccgc cgaaacgcgg accaagaaaa
    23461 gaacccgagc gtgctcacca acagcagcgt catccaaatc ggacccatcg ttttctccct
    23521 gacttagagc gcggaatcag tacgaatctg ggctcgcgct gcgcgaaggt ttaggggtgc
    23581 ggcgcatcga agtcaaacgc cgcgtcctgg tcgcccaaga ctgggagggc ccgttccact
    23641 atgatcctcg ggtgcgccgt ttggggtcga tatttctgct gggagtcgct gcatgtgcat
    23701 cgccggcgat gcgaacggtg ccgcgagtcg tcgacggaga agtggagaac gggcccttcg
    23761 tgtctccgta cgcgtacgag tggttcatcg aaggtgaagc gctcgcggcg aagggacagc
    23821 acgcccaagc ggcgattgcg tttgagaacg cggcggcggc acccgatgac gacgttctgc
    23881 tcatgacccg tttggccgag gagtacgagc tcagcggagc ttaccgaagg gctgaccgaa
    23941 cgctcagctt ggcgcgccgc aacgatccga gctcggcgcg ggtcgccctc gcggaggggc
    24001 gaatcctgca aagcagaggc gcggaagcag aggccttgtc ctcgttcgcc cgcgccatgg
    24061 acctggcccc agcctgggaa gagccagtga tcgagatggc cgtgagccta aacgccttag
    24121 gccatccgca gcgggcaaat gccgtcctgc tcgactacgt cgagaacagc attgcctcaa
    24181 ggtccgcgag cgcccgcaag ttcttgctcg agctcgcgcg agaagcgaaa gatgggcggt
    24241 tgtacgaacg agccctcttg ctcgattcga gctcgaccgc cgagtcccga gcggaggcag
    24301 ccggcgcctt gtcgctcgag gccgggcgcc cggccatggc cgcccgaatc ctgggccgag
    24361 ctctcgattc gacgaaaaac accaatctct ggatacgcgc actctcgcag agcggcgatc
    24421 gagctcgagc gctggacttc ctcgccgagt cccaaagcga tcaggtagaa ggcgacgtct
    24481 ggcgggccga atggctagta cggctaggcg atgcccaacg cgcgttggac tcgctgcaag
    24541 ccatgtccgg atctccgaga gtcgagctga gccgaggcga ggcgctggcc gctcggggag
    24601 agtacgccag cgccgctgca gtcctgtccc gaattccgtt tggtgcgtcg acgttcgaag
    24661 catcgcgcct cgcgcttgtc gagtgctcga cgtcgctagg cagggccgga gccgccactg
    24721 aagccctgag tcaggcgccc cacggatcgc tatctgtgag ggcgaaggtc gcggagttct
    24781 atctcgaaga aggcgatctc cctgcggcgc tgcgattgtt cgatccgaag aatccgaaag
    24841 agcgcgcgct gatcgcaagg ctgttcgagg aagccgggga gttccgtgaa gccgccgcgt
    24901 actacgccag tctgaaaccg agtttcaccg acgggtctcc gcttgccgcc cgagcttcgg
    24961 cggagcgcct agctgctcac ggtcatctcg gaagagccgt ctcagttctg gaaggctgga
    25021 ggaccgctgc gccagacgac ctgtatgctc gcgtccggct ggtggagctg cttctcgcac
    25081 agagtcgaag gaacgatgca atgcgcgaag ggcgcgacgc gctcggcctg atcgaagacc
    25141 agatgctctc ggcgcacctg agcgacctgc tcactgcgca gtcaaacctg tcgcgctgaa
    25201 caggtctcag tactcgccaa acaggtcttg aatgcgcacg tcgagcgccg tcgcgacttt
    25261 gtagagcgag ctgatcgatg cgctggattc ggcgcgctcg atttgtgaaa gaagcgagac
    25321 gctcaatccg gtgcggcgcg ccatctgctt caaggtcagc gcgcggtcct ttcgcagtcc
    25381 ccgaatcgtc tctccgatga cgcgatggag gttttcttcg ggggtgcgca gcagcccctt
    25441 tttccgcatc acgcgatcga gaacttcgcg gaactcatcc gggttgaaag gtttcttcag
    25501 atagtcgacc gcatcgagct tcatcgactg gaccgccgtg tcgagcgatg ggtacccggt
    25561 gaagatcacc accgcgacgt cgctgtcgac ccttcggatt tgctcgagca actcgacgcc
    25621 gcccaggccg ggcatcatca agtcgagcac caccagatgg tagccgcctt ccttgacttc
    25681 attgggcgcg tccttggggt tggcgagcgt ggtcacctcg aagccgtcct tggtgaggaa
    25741 cgtctccatg aactccaaaa tcgcttggtc gtcatcgacg actagaactc ttactggtgg
    25801 gagcgccttt gacatcttgc ctccgctgca attgtgattg cagtgttcaa ttctagtcgg
    25861 ggcgagagga ttcgaacctc cgacctcacg gtcccgaacc gtgcgcgcta ccaaactgcg
    25921 ctacgccccg tatttcaagc tcggcggaat ctagcgttac cgtcgagagc tgtcaacgaa
    25981 ctatcctaca tttacccaca tatagacttg gtatgcgtca gcccagggtc cggcaccctc
    26041 tacccagggt ccgatcgcga gctgcccgag agatccaaag tacaatgatt tcaaaccgga
    26101 agcacgtcca acttggagct cgaagccccg caagagcacc ctttccagaa gcgccgaacc
    26161 ttcctccttg agcggtgagc agaccgcgta gaggagggtt cctccgggcc gcaccagcgc
    26221 ggccgcgttc tcgagcacga ggcgctgtgt ctcacccatg cgggccgcat cgtcggttcc
    26281 agcccgcagt aaaatttcag gtctacggcg caaagtaccg agcccggtgc acggagcatc
    26341 gacgagcact cgatcgaacc cgccctgcac gcctcccgct cctaccgtcc agtcgacgca
    26401 agcggtttcg agccgcgcgg tgttcaagcg gagccgctga agctcagctg gaatctgctc
    26461 gaggcgatgt tcatgaaaat cggtggcaat gacgcggccg gactcaccga cggcttcgag
    26521 gagctgcgcc gtcttgccgc cgcgacctgc gcaggcatcg agcacgcgct ctcccggttt
    26581 tgcctcgagc agcaagccaa tgagctgcgc tccctcctct tgcacggcga acgccccctc
    26641 cgagtaacca ggaagcgcgc gcgggtcacc taccccactg atgcgaaggc ccagcggaga
    26701 aatcgacgtc cgcgacaccg ctgcgtccgg gtgagcggcg agaatcgcct ccgcgatcag
    26761 atcacgatcc tggtcgaggc gaacgcgcaa atcgatcgac ggaggctcat cccctatgcg
    26821 aaggagatcg gcgccgtgct cggttccaat cgacgccgtg agcgcgtcgc gcaaccagtc
    26881 gggaacgaca accgctggcg gcggcgccgg tacggcgggg cgatcggccg ccaccttgcg
    26941 cagcacggcg ttcacgaagc ctgcaagccc ttttccgcgt ttcgcacgta ccagctcgac
    27001 cgtatcgttc acgaccgcgt gcgaagggat gcgccccaga tggtgaagct gatacaccgc
    27061 gctcaacaac gcgatatggg tccactggtc gagtttgccg aggcgtcgag catgccgcgc
    27121 gagcgaggcg tcgagcgcgg gtatcgtcct caaagttccg taaacgattt gtgtcgcaag
    27181 agcggcatcc gatcggccga ccgactcgcg acgaagctcc gcatccagcg tcggcgcggc
    27241 ccaagcatcg tcgtgcacca cccggtagag cacacgggtc gcgattcgtc gggcggcagc
    27301 taccacggcc ggagcttact cgggtcgggc ccgcaagcac ggagattgga ggctaggcgg
    27361 acgcatgata ccgtagcggc cgtgggggac gacggaagtc ggcaagtacc gatcggaacg
    27421 aggcgatgag cgcattggtt ctcgtggctg accccgatcc attcaacctt cgcttgcttc
    27481 acgaggcctg tgaagtggcg ggctacgacg tggtgaccgc agccgatggg cccgaggtgc
    27541 tcgcgatgat cgcgcgtcgt ccgccggacc tgctcattct cgacgccaag atgtcggaga
    27601 tcgatgcatt cgagatcctc cgcatcctca aagccgacgc cggcctgctc gacattccgg
    27661 ttctcctggt cactgacgag agcgacatcg acgcacgacg ccggggtatc gagctcggtg
    27721 ccgaagacta cctcactcgc ccttaccgag tattcgagat tcaacgaagg gttcgcaacg
    27781 tcttgcgtgc gaccagacgg gaccgaatcg gaattccaga gagcacgcct ccagcggacc
    27841 aacgagaccc agtgaccggg gccggcacgc catcgcagct gttgatcagc ctggactatg
    27901 agcacacgcg cgccgaacgg tacgggcacc cattgagctg tatcgtcgta aagctcggca
    27961 actcgaacga gattgcggcc gcaagcggcg acggcacggc cgaccaggcg atggcgcgaa
    28021 tcggcaccgg actcaaggca tgtattcgcg ccgtcgatca tctgttccga ctgcggcgcg
    28081 acgagttctg tatcttgctc ccggagacgg acggagatgg ggcgggctac gtgctccggc
    28141 gcttggaggg tcaggtcgaa agcgggaagc tctggggcga ggatgtcgtt cccagaccaa
    28201 acattcagat cggactgacg agcacgcctg aggaacgcgt caagagcggc gacgatctat
    28261 tgcagcaaac tcgccgttcg ctggccaaga agagctagag cgcggaggcg aagcgaagca
    28321 cccagagcgc cacggcacct gcgcccagcg cgagcgccca tcgattcaga gcaaagcgct
    28381 cgatcacggc gtcgagcgac cagccgcgcc agcccgcaac gatgccaatg gggatgcagg
    28441 cgcatacggc gaaaaagagt gcgagcccca agggattggc gctggccgcg gcgacccaac
    28501 tgccgcggat gccgtgagcg aaagccgtcg tgagcccgca tcctgggcag ggtgctccgg
    28561 tgagcaagag aaatccgcac ggtgggagcc cgagctgcgt gtgagttccg tgacctcggg
    28621 aatcgggctc cagccaggcg gctgccagca gcacgagcac cgagcccagc gcgagcgccc
    28681 accagcccag gcgttgccaa tgcgggttag ccctggtcct atcggggctc gtcatcatgg
    28741 ttcggctcac gccagtctgg attgcgcttc ggttggccgt caatgatcac cacgcagggc
    28801 agcgcgcagt cgggctcacc gatcgactcg tgaacatgcc ttccaaccgg attgtcattg
    28861 ccagcagcaa agtcagcgta gtgcgcatgc aagcacttta cacctccgga tgagcctcca
    28921 acgccaccac tcgggcgaag aggtgcatcc gccggaagcc gcgcgtcccg tgcgcgggcg
    28981 tagccatcat gtctcacttt gagcgcctca ctcaagctcg ggttggcatc gattctcgcc
    29041 tgctccgcac gaacgccccc ctctgcttca atccgcgcaa tgcgacgatg cgccagaggg
    29101 caagtgagcc agtagagcgt cggaaacggc tccccggtgt cgagcagggg aggcacgtcc
    29161 gcgactacgg gaagtccgag actgcagcgt tttagaacgg tcgacgttgc gcgcaacggt
    29221 cgcccgatct gaagggcgag aatctcgcga tccggctcgt cgatgctcat gagatgcgca
    29281 tgcggtcctc gatctcggtc cagaagtcgg actcgccgac gacctcgagc atcatgaccg
    29341 cttccgcgga cgcgcggtct gcgcgcgcgc cgccgtctcg cagaagcgcc gaaatacgct
    29401 ccgcaatggt gagccgtgtg gccagctcag cgcgtgccac ggcgaagttg tggtcctcgg
    29461 cccatttgag cgaccagatc tcatcggcaa tccagtcgcc aaacacatcg cggctcgtgc
    29521 gcggctttgg ggagcgggcc tgagctcgat cgaaggcggc cgcccatgga cgcggaggtg
    29581 gacgcgggct gcgccggttc tggatagcgg agatcatgtc gcccacccag gccatcgcca
    29641 caccgaactg cgacccgtca cgtgctccga tgaattcgtc ggcttgcttg tcccagctac
    29701 ttcccttgag ccgtgggaac ggctccgcgt cgagaattct cagcagcggc tcgcggccgc
    29761 gaagccgggc gaggtactcg acctcaatcg actcccattc ggagaactcg atctccttct
    29821 ttgccgtcat cgggatgcgc ttgatacgcc ggtcggcaac gagctgacca ccaccgttgg
    29881 cgatcgagct ctcgacatcc tcgggctcaa tcagcggccg ttcgcccatc gttctgcagg
    29941 ggcagcgatg ttccgtagtg attcgacccc caaccggggt cgccacgagt cccaatggaa
    30001 atcgacgaca accttccggc ttgaattcgg ggccgagctt ggcgtgcacc gagcaacgct
    30061 ggtccgacat caggaagaca cagccgccgt cggctgcagt cttgagcatg tagtcgtcat
    30121 ggtcgtcgtc ccagcccgcc gactttcgct cgatccggcg catctccgcg acttccttct
    30181 tcgtgagggg tccgatgccg tggatgtccg tgcagcacaa tccatcccca aagcacgtgt
    30241 accgcgcgcc gggtcgcgga agcatgggtc gaactttggt ctttttcttt ttgtttccag
    30301 ccatgaacgt acgtgggctt cctcagcacc cagtaaaagg ggactgtccc cttcttctag
    30361 gatatgattt cgggatggga gcgagtcctt caccgctcgt gggttacaac acgaacgtgc
    30421 gccataaggg aaagctgtat cacattcaaa ccgaagactc gggtgtgaaa cggccacatg
    30481 tcatcactca gcttttcgca gacggcggac gcatcattgc gtcggagaag acatcatacg
    30541 cagaacacgt cgaatccgaa gacctcactg ccatcgtcaa gcagctcatg caagagcagc
    30601 acaagcgagt gttcattgca cttcgagacg gcgcttacga tgaagatggc ccttccgaag
    30661 cacgaagcga cggtcaatcc gcaactacgt cagacttgga agtgacgacc gaggttctgg
    30721 agcacgcggc cgcgcgtctg gtcgagggcg cgccccacta cgagcaggtg cgggccggca
    30781 gtgtatccca gcagagaatc tctcgagcgc cggccgctcc attcggacac gatgtgttga
    30841 gcgaaaagac gctcgacgaa gtcatactct cgtatcttgc cgaggacctc gaaaccggca
    30901 cctgagcgcg ctcggcttga cactctttcg ggcgcgttgt tagctcctcg ccccattgcc
    30961 aacccgagtg aggcgatttc aagatgcccg acgcaaagat ttccttcacc gagaacatcc
    31021 ccaagtcgat agcgaaggac ctcgagaagg ccagagccgt ggacgcggtg ttcttgtttc
    31081 gagtgactgg cgaagaaggc ggcacctgga cggtcaacat gaaagacgag cccggagtca
    31141 ccgaaggcga cgcggggaac gcggagtgca cgatcgagct ttccaacgag gactggaggg
    31201 caatgactga aaagcccggg acagcaatgc agttcttcat gcacggtaag atcaaggtgc
    31261 gaggcaatgc tttgctcgcg accaagctcc aagccgtgat cggctagcgg acatcgccaa
    31321 gatgcccatt atcaaagaac gcatcttcat gcttctgcag gacccccgcg tgatgaagct
    31381 gatgcgggat cccaaggttc aggcaattgt gatgaagggt tttcgctacc ggggtcgtgt
    31441 agaaggggcg ttcgaccagg gcgtgcagcg catcgcgggg aaattgaatc tcgcgacgca
    31501 gcgtgacctt cgtggactgc aacgccggat tcgacacctc gaacgtgagc ttcgcgaggc
    31561 cgaggagcgt ctcaacgaag accaagccgc tcgatgagcg caggtccgta gtaagcgcca
    31621 aagtagaaca tggcgacgag gggcacgatg atgctcagga tcgcgaggat gaccccgagc
    31681 gtgacgaccg cgtagtccca tcggctgggc ttggatgcgt cggattcgtg ctcgcggatg
    31741 agctcgaggt tgcgacgatt cgacttgtag gccgcgtcga agatgtctcc aatgatcggg
    31801 agcgcgccaa ggaccacgtc gaccaatatg ttgaggatca tgcgcatcaa gacgacgcgt
    31861 ggcacccctc ggcgtagcgc caacgtcagg agaccaaccg agccaacgcc agtgaccgcg
    31921 tcgccgatac caggaaacaa gatcccgatg accggatcca atccgaaacg aaagtcggtt
    31981 cccggaatac gaaagcgatc gtccagcaag gtgacgaggc gctccgccca tcgcctcagc
    32041 gctgaaatcg aacggtccgg atcgaccatg ggtggactct aggcgcgcag ttaggcgtcc
    32101 gccagcacgt gatccccaca gacctcgacg aacacggacc gcagacgttc tcgaagattc
    32161 gagcccttct ccacgaaatg atggatgccc agttggtgaa gaagctgcac gtcgtgctct
    32221 tgcgcacctg cgctcaccga aatgatcgag cagccttgcg cgagtccctc gcccttgagc
    32281 tgcatacaga gctccacgcc gttcatccgg ggcatgtgca ggtcgaccaa catgacatcg
    32341 ggttcttctt tctgaacttc gctcagcgcc tcctcgccat cgtaggcgac gcggatttcg
    32401 gatgaaccga tgatctgccg cacgtagaag gtgaggattc tcgcgagatc tgcgtcgtca
    32461 tcgacgatca acaccgatgg tccgcttcgg cccgatctgg cgaccccgcc gcttctaggc
    32521 cgttcgtcga ggatcgcacg cagctgccag gctaccgcct cggcactttg aatgcgcacc
    32581 tgaggattgg ggctcagcat ttcgtgaacc acctggcaga gcgccagagg acagtcgatc
    32641 cgcgcctccg cgagcggcgc aagttgtgca tgctcgtgcg atgcgatcac ttcgcggatg
    32701 gtggttccgg gataaggccg gcctccggtg agcagttcgt acgcaacgac cccgagcccg
    32761 aaaatgtcga ccaggtgtcc gctcccggtc gcagccgtgt tgagcaaggc ttctggcgcc
    32821 atgtatgggg gcgagcccgc aacgcgttgt ttcccattca catcgaactc tggcaacacg
    32881 agaccgaagt ccatgaggac gacccgatgc gcgggtgtga gcatgatgtt gtctggtttg
    32941 atgtctcggt gaaccagtcc cgctcgatgg acgacgctga gtccttcagc cgcgggcaaa
    33001 agaagctgca cgacctcggc aggcgtgaac tgctcgccgc ttccccagcg agtcgacgca
    33061 tgctgcgtga gagaaacgcc gtacacccgc tccatcacca agtagtcgat tccgcgatgc
    33121 tcgccgagcg tatgaacact caccaaactg ggatgctgaa acgccgctaa ggcccgcgcc
    33181 tcgttgcgca gcggcggcgt gagggggttt ggccaggccg ccttgatggc caccctccga
    33241 ttgagcaggt ggtcatgggc ctcgaacacc tggcccattc cgcccctgcc gagcagctgc
    33301 atgatttcat agaccccgtc gagcagctcg ccggtcgcga agatgcgatt ttcgccgtcc
    33361 gtcacgggtt cgcttgctgc ggcggcgttt acccctaggt cgcgcatatc agtatttaca
    33421 tactatattc gtgacactgt aacaggcaag gtcagaggtc gatttgccga atgtcgagcc
    33481 cttcgaccgc ttttccaagg aatctcgagg ccgcagcccc aaattggccg gggagatcgg
    33541 tcaccaagat ctcgagggag cccggaggcc caggcgtcgc cggctctggg ctgaggatct
    33601 cgcccagggt agccgcggtg gcgtcggcgc tgtccacgat cgcaagctca tggcccagga
    33661 gctcggccgc aacctcgtga atcgtaccgg agagcagtgg atagtgggtg cagccgagga
    33721 cgatcgtgtc gacgtccgcg tcggccagtg gctgcaggta tcgccttgcg gccaagcgcg
    33781 ggacctcgcc gctgacccag ccctcttcgg ccaacggcac gaacagcggc gccgcggtcg
    33841 cgatcacctc catggcttcg ttttccgcgt gaatcgcccg ctcatacgcg cccgatgcga
    33901 ccgttcctga cgtcgccagg acgccgattc gacccgtgcg cgtggcccgc acccctgctc
    33961 gcgcgcctgg ctcgatgacc ccgatcacgg gaatgtcgag tcggctttgg agctccccga
    34021 gcgccacgcc gctcacggta ttgcatgcga cgaccagaag cttgatgtcg aagctcagca
    34081 ggcgctccgc gcagccgatc gcatacttga ccaccgtctg cgcgctacgc gtaccgtacg
    34141 gaacccgagc ggtgtcgccg agatagacga ggttctccct cggcagggcc cctgcaatgg
    34201 cgcgggccac cgttaggccg cccaggccgg agtcgaaaat gccaatcggc tttgccacgt
    34261 gcccctagtc gggcaagctt acacagccgc cccgaaagag ccaccagcgc ggtggtctct
    34321 gataggctga caagcacatg cccgcactgg cgctgatttc gagagatccg accctctacc
    34381 aagagctcgt cgccgcgctc gacgggacag cttggtctgt cgaatggctg ggcgcggagg
    34441 tcctcgacgg gggcgagctc catcaagagg tggtcttgct ggacgcgacg catcccggcg
    34501 cggagacctg gagcacgcga ggatccaagc cggttcttct tctagtggac ggcacgggca
    34561 cgcaagcctt ggaagagcgc gccgacgaca tcatcttcag accgccggga cgacgggagc
    34621 tcgaggcacg actcgagcga ctccatgccc gcacgccgct cgacgagttg cgacgccagt
    34681 acgcaaagac ctttgcatcc atggcgccgg gcatcgtggt catcgacccc gacctccaga
    34741 tggtattcgc gaatccacgg tcctacgaga tcgtgggaca tcgcgaagag acgattgctg
    34801 caagcggtat cgcacgcgtc gtcgcgccca aagacctgcc ccgtgtccga gacgctcttt
    34861 cgcaaggcca gtatccgcgt ggaatggaca ttcacttgat ccgccgtgac ggaacccaga
    34921 tcacttgcag cacatccttt gcgccactga tcggcgcaga agaccacacg gtaatctcct
    34981 ttgaagacgt cactgacatc cgggcgactg agcgggagct gctcaagacg atggagttct
    35041 tggagtcgtt gatcgaggcc agcgtcgatg cgatcatcgc cacggacatg gagcaaaggg
    35101 tgatcctgtt caatccgagc gcggagcgag tttcggggcg acacgtttct gatgtcgtcg
    35161 gcaaagtgaa gctagaagac cttctcggtc gggcgtcttc cgagctggtg actcagcgcc
    35221 tgctcgcttc ggatcacggc ggcgaggggc gactcgaacc aacgatgctc gacctgctcg
    35281 acatccacgg tgctcgaatt ccggtccagt tgtctgcctc gctgatttac gagcacgggg
    35341 aaccatcggc catcgtgctc atcttcgccg atctgcggga caggatccga gtcgaagaac
    35401 gtttggcaga ggcacagaag aagctcgcgt tcacggaaaa gcagggcgtg ctggcggagc
    35461 tcgcggggac agccgcgcat gagctcaacc agcctctaac cagcatgatg gcctatgcag
    35521 agctcttact gagacagtcc gaggagggct cgagcggcgc caaagcggct cgagtgctga
    35581 ttcgcgaggc ggagcgtatg gcggaaatcg tgcgcaagat cggaaagatc acacgatacg
    35641 aaacgacctc gtatgtagga caccagaaaa tcttcgatct cgatcgcgcg gtggacacgt
    35701 ccagcgaccc aaggaccaag ggctgagcga tggacgactc agctcatcag aagcctcccg
    35761 ctcgacgcag cctggaaccg cgggtttcgt ctgtgaaatc gatcgtgact ccgcggcgga
    35821 gggcggcgga gggtgcaacg cttcaagacg tcctcgagtc gctgaatcag gcgcagcgcg
    35881 aaacgaacgg attcgtgact gacgagaaaa tcttgcgtgc gtacgttcac gcgctggaga
    35941 gactctaccc caacatacgt ttcgcgatgc gactgtcccc ccgcaacttg gagcgtgctt
    36001 ccatcattca agccaccact caccgcgtca gcccggacgc gatggacgtg ctttcaatca
    36061 ccgaagcggg actacggcgc gcggggtgtg ccgacacgct ggagccgcca agcgaaatcg
    36121 aattgcgacg aacttacgag ccgatcctgc tcgccgggcc agacgaggag tcgctccctg
    36181 ccgatggctt tgacgcaccg ctcttgcgta acgacatcgt gttcggcgtg ctcgctgtcg
    36241 agttcgagcg tggaacggac ctcccctcgc gactcgaaga tgcggtgctg atgacagcca
    36301 ctcaggtcag cgcggcgatc gagattgcac gacttcgccg cgaatccctg catcttcgcg
    36361 actatctcga aaaacttctc gagcacgcca acattccagt gctggtgatc ggccgcgatc
    36421 ggagcatcag ggtggtcagt ggcgcatttg ggcgaataac cggattggat cgcgccgatt
    36481 tggtcgcgaa agacgtcttg cagctcgctg gccagagcga acgagtgcgt gttctgtcgg
    36541 ccttcgtgag cgcgctgcgc gggcgggacg tgccgccgtt cgagctcaga ctcccgaagg
    36601 ctggaggcgg aaacgcgcgc cttcaattca cactcgcacc gatcgtcgat tccgagggca
    36661 gagtcgccgg cgtcatcgcg atcggacgag accgaaccga agtacgggag ctcgaggggc
    36721 aagtcattca tgctgagaag ctggcgacgc tcggccaact tgcggcgggg atcgtgcatg
    36781 agatcaacaa tccgctaacg agcatctcgg tctacggtga gtacctgttg agcaagctct
    36841 ctcgaaccgg tgcggaacag tctgatttga accgagtgga gcgcattctc cgaagctccg
    36901 atcgcatcat gtcgttcact cgcaacctcc tgacgtatgc gcgaccatcg aaagaggagc
    36961 cccagccagt ctccctgaac gacgtcgttg aagaggccgt ggtcttctgc gagcacatcg
    37021 ttcgtgaagc gggagtcgag gtctcgtgcg actacggaga acaactcccc gctgtgaacg
    37081 ccgtggccgg acagctacat caggttttcg tcaatctcat caccaatgcg tgcaatgcgg
    37141 cgccagaaga aggcggcttt gtgcgtttgg caacgcatct tcacggtcac gatcgaattc
    37201 acgtcgaagt ccacgacaac ggcgtcggca ttcaaccgtc ggagctcagc agggtgttcg
    37261 agccattctt ttcgacccgc cgcaaaggca agggaactgg gctcggtctt tcgatcgtaa
    37321 agaacatcat cgaagagcac cggggcagca tcgagatcca gagcgaggtc ggcaagggca
    37381 ccaccgtcct ggtcacgctg ccctgcctag acatctaagc ctctggaatt acgaactttc
    37441 caggtaacgt tgggcagctt tgcacctata tttggaatca agccgcgccc tcggaggtta
    37501 ggcagtccat gggaggtatg tgcatgttcg gaaccacgtt tcggcggccc tgggtcatcg
    37561 ccctggccgc gacttcggct atcgccatcg ctggtggcgc tttggcgtcg cccaagaagc
    37621 agctcatcga agatgacgtc ctcgagcggg cgctcgcttt cgagactgcg ctgacgagcg
    37681 tcgctgaggc ggtctcgcct tcggtcgtca gcattcaagt cgaggtgacc aggcccgaag
    37741 ccaacggcat ccctttcttc ttcggcggcc agagtggcag cggaatcgtc cgtgggggcg
    37801 gctccggcgt catcgtgcga tcggacggtt acatcctcac gaacaaccat gtcgtgaacg
    37861 gggccaaccg catcgacgtc cgcttacaga acggcaagag cttccaagcc aagctcgttg
    37921 gagcggactc tgcaacggac ctcgctatct tgaagatcga cgcgcgaggt ctgcccgaag
    37981 ccacgtttgc ctcgtcggag gacgcgcggg tcggtcagct cgtcatcgca atcggctcgc
    38041 ctttcgggct cgactacacc gtgacgaccg gagtgctgag cgcgaaaggt cgcgacggga
    38101 tcggcgccaa cgagatcgag gactacctgc agaccgacgc cagtatcaac cctgggaact
    38161 cgggtggacc cttggtcgat cttcggggca gcgtgctcgg catcaacacg atgatcgttg
    38221 ggcgcaactc gggtatcggc tttgcaatac catcagaaat cgcgcagcgc gtggccagcc
    38281 aattgatcca gaacggctcg gtaaagcgcg cttggctggg tatttcattc caggagatca
    38341 ctccggagct cgctgcccac ttcggaggcg acttcgaagg cggagcgctc atcaacgcgg
    38401 tcgtgcccga cggaccggcc gaaaaggctg ggcttcaagc gggtgacgtc attacggcca
    38461 tcggcgacca gaggatccaa gaaggtcacg agctcttgcg caccgtcctt cgccagggtg
    38521 tcggtgagcg agtcgtgctg gaggtgcagc gcggaggcaa gacgagcaag gtcgcgctcg
    38581 tgaccggtgc gcggccgagc gggaacgagc cccagtcact acagcaaggt accgaggacg
    38641 acggagtgct cggtctggcg ctggaggatc tcaccccaaa ccttcgcaaa cggtttgggt
    38701 acgaaggtga cgggcgcgtt tttgtccgcg gagtcaaacc cggatcagac gcggatcggg
    38761 ctggacttcg ccccggtgac atcatcctgc aggccgatcg agagccagtg cgctccgtgg
    38821 ccgatgttcg cacggcgctc aaagacggtc aggcgttgct ctacatcgag cgcgaatccc
    38881 gacggttctt ccaaccgatc gctcgctcga aatagcgcta tcgcgctatc gtgatctcga
    38941 tcgcggggcc gcgcgcttcg acccggtatg cggggtggcg ccccgcaacg aatcgaaccg
    39001 tcagctcgga gaagtccccg cgattgagaa tgctggcgtc ttcgacatcg ggatgtgcgc
    39061 tggcgatggg acccgcccgt gagagtgaaa gactgctagg aatcgtgacg gaaaacccgt
    39121 ccgtttcgat gatcccatcc atgagcgtca ccggattgct catgcgcagc acgtacttct
    39181 cgccgcctgg cacagcatct gcgccgaacg cgataccgcg aacgcttttc ggaggcagcg
    39241 gtgcgccctg agcttggagt tgggtggcct cgatcggagc cgcgctctcg gtcttggtcg
    39301 gggtcttcgt ccccgaagat gcgcccgtcc ctttcacgct cttggttgcc tcgactggca
    39361 actcatctac ctcggtcgtg gcctctgtca ccgcctcgtt gtccgttccc gagctggcgg
    39421 ctgcccatag atagatcccc cagccggctg ccatgatccc gcatgccatc gctgcgacga
    39481 tcagataccg gcgacggtct cgccgagtgt tcttgagatg cgcgcgggca gcctcactgc
    39541 gaggcacctg cttgcgtcgc ggcttgtcgt cgagcctcga gcgcagcgcc gcgatgaatc
    39601 ggcctgccac cccgaacatc tctttcgcac tcgaagcggc gcggggaatt agcccctggg
    39661 tcacggccat gcgggcgccg ccaacactgc tcgcgagctg atccgcagcg gatttcgcgt
    39721 aggcgcggag acggtccaag gcgggctcgg acccgggctc atctgcggcg gcatcatagc
    39781 gcacgatccg gggcgcatcg tgtatccgtt cttcccgagg tcgcggggct acctgagccg
    39841 ccgcgggcat gtcctcggga acggtatccc ggtctgcacc ctcttcggat acgcccatcg
    39901 ccggatcgac ctcgtagccg atgctcagca ccagcttggg ggtcttctca tccatcttga
    39961 gctccacggc ctcgaggtgc ccgcggcgtc ccgcgctttg attgagcacg gtgctcccaa
    40021 tggccaagaa gggaagtggc tgctccactg agagcgcggt gcgtgtctcc gatcggatct
    40081 cggccatgat gggagagcca actccgtcca gctgcagttg cacgatcggt gccttggctg
    40141 tggtgcggcc ccagcgctga atcagtgctt cgatgcgcga ctggtcgtca ttcgaaagat
    40201 gggtaaatcg aatcccgaac tcgcccacat gtggaccgct ctcgtgtgcc cagacgaccc
    40261 ggccgatcat ttcgatcgcc tctccgcccg tggggttgtc caattggcag cgcagcgaag
    40321 aacccacctc cggcacgacc gaggcgcgca tcgagagtcc accagctccc atattgatac
    40381 cgtcggcctc gaactcttga tggaagcctt cggggtggag cttgatcaaa acctcgtcga
    40441 gtggcacgcg ctgctgccga ggtgcttgat agtaacccgc ggatgtgttc atcgaatgct
    40501 ctcctcagcg accggtgagt cgcccccatc aggactggtc ccaaaactcc gcgaacccgt
    40561 ccacttttct gcaacccgct tccttgaccc attcgcgacc ggaccccagt cttggcggct
    40621 acccatgggc gtgcgaatca cggtgcagtc gctttggacg ggggccgacg cctccgcctt
    40681 ggtgtacgag ttcacgcaat ctcgaatcgt gattgggcgc tctcggagcg cagacgttca
    40741 gctaccccac gccgcagtca gcggaagtca tgcgatcatt cgtgagcaag acgccggcta
    40801 cgttctcgtc gacgagggct cgacgaacgg gactcgcatc aatgaggtcc tcgtcgtccc
    40861 gggacgcccc aaaacccttc gcagccggga cctcgtcgac ctcggcggct atcggctttc
    40921 catcgatgta tgcgttccgg tatcgcagcc gatgtcggcg aggctcaccg ccgaccatgc
    40981 aaagcggatt ctctcggagc aactcggaag cacgaatgcg gacgagatcg acgccgagct
    41041 gcgcgcgatc cagagcggtc ccgacgagca tgtcgagctg ctgccgatca ctggagaacg
    41101 ggtgagcgag gctccgctcc cgagggagtc gcgtccttcc aagcctcggg aatcgagacc
    41161 tgagagcgga cctccgccag agcccgttcg actcgcgcga agcgagctcg ccgtctacgc
    41221 gcttgcctcc gtcgtgatca cgatctgcat cgtcgcgatg gccttgctca tgaagcacta
    41281 ggcgttgcgc agatagatga ggcgaagccc ttctagagtc aggaactcgt cgatttcgtc
    41341 gatcgtctcg ctgtcgggct cgatgagact cgcgagtccg ccggtgccga ttacggcgac
    41401 ctcggactcc atttcggcag ccagacgccg gacgagacca tcgaccaggc cgacgtagcc
    41461 gaagacgatg cctgcctgca tcgaatgcac ggtgttgcga cctatggccc gcggcggccg
    41521 cgcgatctcg ctccggggca acctcgcggc acgggagaaa agcgcattgg cactgatctg
    41581 catccccggc gcgatcgccc ctccgaggta ttcacccttc tcggagacgc aatcgaaagt
    41641 cgtggcggta ccgaaatcga ccacgatcgc ggcgcccttg acgcgttcga atgccgcgac
    41701 cgcgttgacg atccgatcgg cgcccacttc acgcggattc tcgtacagca ctggcatgcc
    41761 agtcttgatc ccggggccca cgaccataat ctcatgatcg aatgccctat cgacggcttc
    41821 gatcacggta tcgctgaaag aggggaccac cgaagcgaga atgctcgccc gcacgtcgga
    41881 gcggtcgacc ccagcaagcg acaagaggtt cagcaagagg acgtgatact cgtcgctcgt
    41941 gcgtcccttg gccgtttcga tgcggaagtc gtggacgagg cgttccccgt cgtagaggcc
    42001 gaggacggtg tgggtattgc cgacgtcgac gaccagaagc atctagcgag gccgcaagga
    42061 cagagtcagg tcgagcaggt cgccaaaggt cttcgccctt gggaactccg gtggatcggt
    42121 ggcgtagacc acgggctgtc ccgtcggagc aacgctttga ggtcgaatcg cctggggccg
    42181 gtcgctcgaa tgggcagccg cgggcgccgc agaggatggc cgaatcgacc gggcacgggg
    42241 ttcgatcgac agcggccgct ccaccctcgg tggctgcgac gagtagcggg tggggctgtc
    42301 catcacaggc gaggtaggcg gctccagcac gggcgaggac ggcaactgcg ttggaaaaga
    42361 gctcggaaac ggtgtggctt gagcacc
//
LOCUS       DASRZF010000379         6245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3387124, whole genome shotgun sequence.
ACCESSION   DASRZF010000379 DASRZF010000000
VERSION     DASRZF010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 6245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6245
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3387124"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VFG22_16880"
     CDS             complement(<1..390)
                     /locus_tag="VFG22_16880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET6417974.1"
                     /translation="MLNRSVALEGLVLVAVLLLTVCGIGSFGIWDPWELVVAEAARNL
                     SASGADSSLHTPLTTWLVAAAFDAFGAHEWSGRLPGVIAAILSGVLALVLLARPYGRR
                     AGIIALAVMASTPMFLLNARLLMGDAIA"
     gene            complement(419..1909)
                     /locus_tag="VFG22_16885"
     CDS             complement(419..1909)
                     /locus_tag="VFG22_16885"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HET6417975.1"
                     /translation="MSADPPKEKEPSGLRPLPSVDMALLPNELRAQRTRRLVVSALVG
                     AVVLGVLLLWIARSVIPQTWAWMEDALGRQDAKLAADLGVPEGLAPLDADAGIDETLS
                     EAEALAISRSRPNARYETPFGTVPSFRGALLNAGLENDECTAIEQALQHIVDFRRCRP
                     EHTLVVERDENADLKLFEYHPSPTEFVVVSRGEDGSFRAEQIRIKVERTPIARAASVE
                     TSIGDGLVGLGLPAGLATFFVEAFEGQINFALQARKGDVFRIIVDEERVDGEFLRYGR
                     VHALEYDGQRTGKVQAFYYAPANSTGQFYDDTGRAMQGGWLRTPLRYERLSSGFNPRR
                     FHPILKRTMPHLGVDYAAPTGTPVWAAAHGRVVFAGRKGANGNLVIIRHGGGFETAYA
                     HLLRIKGGIRPGKLVKQRELIGFVGSTGRSTGPHLHFGLKKYTRAIDPLSELNGPGLR
                     MSARELPAYKETVASWQAQLGSIDDAPKLVPGADEPLVEQVDEIMD"
     gene            2091..2165
                     /locus_tag="VFG22_16890"
     tRNA            2091..2165
                     /locus_tag="VFG22_16890"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2123..2125,aa:Val,seq:gac)
     gene            2299..3435
                     /locus_tag="VFG22_16895"
     CDS             2299..3435
                     /locus_tag="VFG22_16895"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET6417976.1"
                     /translation="MVKHALVVAFLLTLLAPAWVSAEEPSEGVSKESGEAAPNAFLDG
                     LEWQVMASGFYLFNAHRVAGPYNDLNYPYTGYMGFGLNFAGGDVSYTRKKFGLTLGLR
                     WGSAASQLTALAPVKQAYASWMPHRKLTFDFGWFDTIYGAELHDEWHNPTFTRGALYF
                     LMQPFNHLGLRITADLNEKLALKFLVVNEFVGVGRLAGTTIDTNETPALGVQLNITPV
                     DSVTLQLGYLTGASGLNGNRAWGQFFDLILTARIRRFTLTFNGNATVDPPVSSGQYSM
                     GAALSGDMRVHDHWRVGARVEYIAGTVALPAGRDPDLLTVTATVRYIPVQYLVISLEP
                     RFERAQQEIFFTRDSQTDASTGDRIADSDVYFGFVLGVSAYIGN"
     gene            complement(3703..4620)
                     /locus_tag="VFG22_16900"
     CDS             complement(3703..4620)
                     /locus_tag="VFG22_16900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET6417977.1"
                     /translation="MTRPRIVDPGATLAISRRTTRRHFLLHPDEARQMEQVYWYCLAY
                     AAKLHGIVVHAACLMSTHSHEVVTDVRGEYPKFLHTLHRYLALCTKAYRGWPEEVFNK
                     NSSGAHALLTPDAIIESIAYLIANPVEAGAVRYAKDWPGAHTLPGHVGTRVIRVKRPK
                     HYFNPENTDWPDELELRLEMPVVLELDYGAEVARERIAERVRIKEHRAWSEAKRNGLS
                     FIGPRRVLKLAHTKRAKSYEVFGSLNPQFAAAGNRAAATEAVKRLRAFKAQYARALAK
                     WTAGDRNACFPRGTWWMRVCHGARCGPGP"
     gene            complement(4950..5243)
                     /locus_tag="VFG22_16905"
                     /pseudo
     CDS             complement(4950..5243)
                     /locus_tag="VFG22_16905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_043767036.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(5799..6245)
                     /locus_tag="VFG22_16910"
                     /pseudo
     CDS             complement(5799..6245)
                     /locus_tag="VFG22_16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567368.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
BASE COUNT         1071 a         1881 c         2073 g         1220 t
ORIGIN      
        1 tgcaatcgcg tcgcccatga gtagccgcgc gttgagcagg aacatcgggg tggaagccat
       61 caccgcgagg gcgatgatcc ctgcgcgacg gccgtaggga cgcgcgagta gcaccaacgc
      121 gaggacgcca ctgaggatgg cggcgatgac tcccgggagg cgccccgacc attcgtgcgc
      181 cccgaatgcg tcgaacgccg ccgctacgag ccaagtcgtg agtggtgtgt gaagcgagga
      241 gtccgcgccg ctcgccgaga gattgcgggc ggcctcggcc acgacgagct cccagggatc
      301 ccaaatcccg aacgatccga tgccgcaaac cgtcagcaag aggactgcaa cgagcaccag
      361 gccttcgagc gcgacggacc ggttcaacac cgccggcagc ctaaccctcg ctcccgcctc
      421 agtccatgat ttcgtcgacc tgctcgacga gcggttcgtc tgcgccaggg acgagctttg
      481 gtgcgtcgtc gatgctgccc aactgcgctt gccagctcgc gaccgtctct ttgtatgcgg
      541 gcagctcgcg tgcggacatc cgaagaccgg ggccgttgag ctcgctcagc gggtcgatgg
      601 cgcgcgtgta cttcttgaga ccgaagtgca agtgcggacc ggtcgagcgt ccagtcgagc
      661 cgacgaagcc gatcagctcg cgctgcttca cgagcttgcc aggacggatg ccgcccttga
      721 tgcggagcaa gtgcgcatag gcggtctcga atccgccgcc gtgacgaatg atgaccagat
      781 ttccattcgc gcctttgcgc cctgcaaaga ccacccgccc gtgtgccgct gcccagaccg
      841 gagtgcccgt gggtgcggcg tagtcgactc cgaggtgcgg cattgttcgt ttcagaatcg
      901 ggtggaagcg cctcggattg aaacccgacg agagcctttc gtatcgtagc ggtgtccgca
      961 gccagccgcc ttgcatcgcg cgaccggtat cgtcgtagaa ctgccccgtg gagttggcgg
     1021 gtgcgtagta gaacgcttgc actttgccgg tgcgctgccc gtcgtactcg agcgcgtgca
     1081 ccctgccgta gcgaagaaac tccccgtcga cgcgttcctc atcgacgatg attcgaaaca
     1141 cgtcgccctt gcgtgcttgc aaggcgaagt tgatctggcc ttcgaacgcc tcgacgaaga
     1201 acgtcgccag ccctgcggga agcccgaggc cgaccaggcc gtctccgatc gaggtttcca
     1261 cggatgccgc tcgcgcgatc ggtgtgcgtt cgaccttgat tcgaatctgc tcggcgcgaa
     1321 aggaaccgtc ttcgccgcgt gagaccacga cgaactcggt cggggaaggg tggtactcga
     1381 aaagcttgag atcagcgttc tcgtcgcgct ccacgacgag ggtgtgctcg gggcggcaac
     1441 gtcggaagtc gacgatgtgc tgcagcgctt gctcgatggc ggtgcattca tcgttctcga
     1501 ggcctgcgtt gagcagtgcg ccgcgaaagc tcggaacggt accgaacgga gtttcgtagc
     1561 gtgcgttcgg ccgcgaacgg gagatggcga gcgcctcggc ttcgctgagg gtctcgtcta
     1621 taccggcgtc cgcatcgagg ggggcgagtc cttcgggaac gccgaggtcc gcggccaact
     1681 ttgcgtcttg ccggccgagc gcgtcttcca tccatgccca ggtttgcgga atgacactcc
     1741 gtgcgatcca gagaagcagt acgcccagga cgacggcgcc gaccagcgcg ctcacgacga
     1801 gtcggcgcgt ccgctgcgcg cggagctcgt tagggagaag cgccatgtcg accgacggca
     1861 acggacggag cccgctcggc tccttctcct tcggcggatc ggcgctcatc gaacgctcca
     1921 cttatcagcg gccccagtaa taagcaaagg gcggagcaaa gagcggagcg aagggcgccg
     1981 cgccatgcga ctgtgcagcg gcgggccccg agaagcgccc gctttacggg gttgacaccc
     2041 ctcagggtgg gggttaagac gccgcttcca ccaaggtgga cttgataaac aggcacgtag
     2101 ctcagtggga gagcactacc ttgacacggt aggggtcggc agttcaatcc tgcccgtgcc
     2161 taccacgctc gttgcgtggc cacgaaatcc ggtaagcgtg tggctcctca ttacggcctg
     2221 ggcccgcagc gccctccgtg gcaaagtttc aaccaccagg cgctgcgacg cgggtatggt
     2281 attcccagcg cctggtcgat ggtgaagcat gctctagtcg ttgcgtttct tctcacgctt
     2341 ctggctccgg cctgggtgtc cgccgaagaa cccagcgagg gcgtttccaa ggagtccggc
     2401 gaggcggcgc ccaacgcatt tctggacggt ctcgagtggc aggtcatggc gagtggcttc
     2461 tatttgttca acgcgcaccg cgtcgccggg ccgtataacg atctcaacta cccctacacc
     2521 ggctacatgg gcttcggtct caacttcgcc ggaggggatg tcagctacac caggaagaag
     2581 tttgggctca ccctcggcct acgctggggc tcggcggcat ctcagttgac cgccttggcg
     2641 cccgtgaaac aggcgtacgc aagttggatg ccgcatcgga agctcacgtt cgacttcggc
     2701 tggttcgata ccatctatgg tgcggagctc cacgacgagt ggcataaccc aacgttcact
     2761 cgcggcgctc tctatttctt gatgcagccg ttcaatcacc tgggattgcg gatcacggca
     2821 gacttgaacg agaaattggc gctgaagttt ttggtggtga acgagttcgt cggagtgggc
     2881 cggctcgcgg gcactacgat cgacacgaat gaaacgccag cgctgggcgt gcagctcaac
     2941 atcacgccgg ttgattcagt cacgcttcag ctggggtatc tgacgggggc aagcgggctc
     3001 aacggcaacc gggcgtgggg acagttcttc gacctcattc tcaccgcccg aatccggcgc
     3061 ttcacgctga ctttcaatgg caacgcaacc gtggatccgc cggtcagctc cggtcagtac
     3121 tcgatgggcg ctgcgctttc gggtgacatg cgggttcacg atcactggag ggtgggtgct
     3181 cgcgtcgagt acatcgcagg aacggtcgcg ttgcccgccg gcagagaccc ggaccttctc
     3241 acggtcaccg cgaccgtgcg ctacatcccc gtccagtact tggtgatttc gctcgagccg
     3301 cgcttcgagc gcgcgcaaca ggagatcttc ttcacgcgcg actcgcagac ggatgcgagc
     3361 actggggaca ggatcgcaga tagcgatgtt tattttgggt ttgtgcttgg tgtcagcgcg
     3421 tacattggca actaaagacg ggccggcgtg cgaagatccg accgtgcgaa aaagatccga
     3481 ccgtggttgg cggtgagact ggctggcgtg aagggggcgg agggcttgat ttaaacgcgt
     3541 ggtgggaacg gacgtgtggg ggatgcgcct gcgtcgcgct agttcgactc agaaatggct
     3601 tctatcacca ttgtcgcggg gcgaattgct actcgtggcc gcaaccggac gccgccacgg
     3661 tggttgcgtg gccgggagaa cctctgatca tctggcgagt cgctatgggc cgggtccgca
     3721 gcgcgcgccg tggcagactc gcatccacca ggtgccccta gggaagcatg cgtttcgatc
     3781 cccggcggtc cacttggcca gcgcccgtgc gtactgcgcc ttgaaggctc gaagccgctt
     3841 caccgcttcg gtcgccgccg ctcgattgcc cgcagctgcg aactgggggt tgagactccc
     3901 gaacacttcg tagctcttgg cgcgtttggt gtgagccagt ttcagaacac gcctcggccc
     3961 gatgaatgaa aggccgttgc gtttggcctc gctccacgct cggtgctcct tgatgcgcac
     4021 ccgctctgcg atgcgctcac gagcgacctc ggctccatag tcgagctcga gcaccaccgg
     4081 catttccaag cgaagctcga gttcgtcggg ccaatccgtg ttctcgggat tgaagtagtg
     4141 tttaggacgc ttcacacgga tcacgcgcgt gccgacgtgc cccgggagtg tgtgggctcc
     4201 gggccagtct ttggcgtagc gcaccgcccc cgcttcgacc gggttcgcga tgaggtaggc
     4261 gatcgactcg atgatcgcgt caggggtcag cagcgcatgg gccccgctac tgtttttatt
     4321 gaacacctct tcgggccagc cgcggtaggc cttggtgcat agcgcgaggt agcgatgcag
     4381 agtgtgcagg aacttcgggt actcgccgcg cacatcggtg acgacttcgt gggagtgcgt
     4441 cgacatgagg catgcggcgt ggaccacgat gccatgaagc ttcgcggcgt acgccaggca
     4501 gtaccaatac acttgctcca tctgccgcgc ttcatcggga tggaggagaa agtggcgtcg
     4561 ggtggtgcgc cgggagatcg ccaatgtggc accggggtcc acgatgcggg gtcgggtcat
     4621 gcaggggagg cagaagcgaa tggtgtgccg agtgcccttc gggtgatctc agctgcttcg
     4681 cggcatcatg gtgccgattc gggaatggcc gccggtgggc gacacctcgc gaaacaacac
     4741 ggcaatctgc tgatgcctgc gtcgtttgga ctaatgtcgg ttcgagcttc tactacagca
     4801 gtcgtgttct gtagactttt gcagcatttc ggtggcggtg ccgtgaacgc ccaccggcgg
     4861 gcgacgcctc acgaaaccaa acggcaatct accggtgcct gctgcgtttg ggccaatgtc
     4921 ggttggagct tcttgagctt ctcttcgagc ttctccccgg cgagggtcgg accgaagtcc
     4981 cggtagcgct cgcgcacaag accgaggacg tacgcttggt acgcctcggg aatccgattg
     5041 ggtgcaggtc ggccgcggcg tttgtctcgc aagcctgcgg gtccgtctct ttcaaacgct
     5101 cgcaggagcc tccgaacctg acgctcgctt cggccgagac agcgcgccgc ctcgacctgc
     5161 gtcatacgtc gctccgcgag ccgcgtgatg atcgccagtg tgtccatttc tctcgtgctc
     5221 agggtgaaga gatcgccggt catgggccgg gattgaaccc tggaccggac atctatgctt
     5281 gggagaaacc ggacactatg gctgggggct tacagcccat gtcaaaacca aactcaggcg
     5341 tcgggtcgct ctccaagatt gaaatgtcgg gtagggggtg ggtgcgagcc ggctgggagc
     5401 agcgtttgag ggcggtttgt gcaggacgaa cacgtcggtg cgtctggggg aacgtcgcgg
     5461 tggggcagga ggcggatggc ttgagagggc gtcgataggc attgcagggg cctgccggct
     5521 cagggtcccg gaggcggcaa cttgtttgcg aagcggtccc ggacgatgcg cttttcacgc
     5581 ttgctcttgg cggagcggac gagctcttgc tcgcgttgtt gttggcgctt gcggatttcg
     5641 gtcagggcac cgctcaggag tttgttctcg acgatggagc cttgggtcac gcagccgtct
     5701 ttgtgaaagg ggcgagcggc aagcgcgacg cctcgatatc gaatcgacac gccgccatcc
     5761 tcgtactcga cgaccgtcac gcgcttcttc gccacagctt tcgcttcctc ggtgggctcg
     5821 agcaggtaca tcgtgcgctt gtggtgcacg gtgagctgcg cggtcacgcg ccgctcttcc
     5881 tgccaggtga agatgtcgtc gagacggtcg tgctcgagca acggacgatg tgcatcgtgg
     5941 tcgtttcgcg ctgagcgtcc gaagcgtcga ttgtagtcgt ccatgaactc gggcaagtac
     6001 cgattggcgt cgtccatcgc gcagataccg cggagccgga gctctttgac gagccggtct
     6061 tgaagcgtct ggtgggcgcg ctcgacccgg cctttggccg ccggcgtatt ggcgcaaatc
     6121 acgtcgatat tgagctcgct catcgcgcgc ccaaactgcg tgaagccgtc gccggctttc
     6181 gggcgcttgg cattgacgcg gaacactccg gctttgtcgc tgtaaagcgc gaccggcttg
     6241 ccgtg
//
LOCUS       DASRZF010000380         5621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3725493, whole genome shotgun sequence.
ACCESSION   DASRZF010000380 DASRZF010000000
VERSION     DASRZF010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 5621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5621
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3725493"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VFG22_16915"
     CDS             complement(<1..423)
                     /locus_tag="VFG22_16915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417978.1"
                     /translation="MTDFRIDVVVDPGKAQKGLKTVEKSLDSVEKEAAQLRQAIVGAL
                     QARDAGVIGELKQLNANLTRSEGSARDLKQEISQVGSRVNTKPIDQIDTSLQRAESRA
                     SSLGSTLRRAFAVLGVSLGVRELVGFADQLTNLQNRLRI"
     gene            complement(420..593)
                     /locus_tag="VFG22_16920"
     CDS             complement(420..593)
                     /locus_tag="VFG22_16920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417979.1"
                     /translation="MCNFFANVILSLDAQYREHLTSEGEKEAKKQERQAKREASQKTT
                     GKMSGSMRAGRRR"
     gene            complement(848..1339)
                     /locus_tag="VFG22_16925"
     CDS             complement(848..1339)
                     /locus_tag="VFG22_16925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417980.1"
                     /translation="MTATKRKPRRTAVGSNGHFDFGLDELEVAGKVRRLEMPMVSPDA
                     YLLLVSTHDNPQYEEMLIRQSALESLKVGEDDEDPVEKDARELAFERERYARTIVKGW
                     GGIRQRGSNAQVPFTLEASLAFFQQIPKYLFNRVRAFCLNTKTFNGSGSESELDPRGL
                     AKN"
     gene            complement(1406..2992)
                     /locus_tag="VFG22_16930"
     CDS             complement(1406..2992)
                     /locus_tag="VFG22_16930"
                     /inference="COORDINATES: protein motif:HMM:NF039819.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HET6417981.1"
                     /translation="MARVLTNNTSLRVATETSIGVLPGTPQWTVVEFDTIGAYGAVLT
                     TTVRRPISQDRGRKKGTVTNLESSVEYDTDLTFEAFRLFAEGFMFSEFTNVEFNLRAT
                     TGTVPPPAVAAGGVFTIDAASTLLAGKMQYGAATVQTLLFAKGYTNAVNNGLHVLAAD
                     VAGTNTSVDVSTVLVDETPPSNATLEVCGIRTDDLTMAISGSTLTLTSAADLNWTTIG
                     LTVGQEIHIGSMDVSGVVQNAYDDGGTDDVYGYARITAITATVLSCDKIDPIIAAASG
                     SSPFSPETIDIMFGNFLRDVAVTADSDDTRYLERTFQFEAVFPDLGGIGTDQYEYAIG
                     NFANELSFNFPLQDKSTVNFGFIGTNADDITGTRKTGASTAKSPLRNTAFNTSSDVQS
                     LTTDLISSVSDVCFKSLTLTARNNVSPEYCLGTLGATFVNAGLFEVDVEGQMLFTSAS
                     IINAIKNNTTVTFASIFRNEDGAIAYDIPEATASGGGREYPLDQSVLVNLVLQAFTSD
                     TFGYSMGISLFPAVPGVLSS"
     gene            complement(3101..3523)
                     /locus_tag="VFG22_16935"
     CDS             complement(3101..3523)
                     /locus_tag="VFG22_16935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417982.1"
                     /translation="MARNQTRIVVGDLGDSINRVMRRLQLRLYQSLSTPTPVDTGFAR
                     GGWTPSTGAPDRSGPADQPSDRSAAIQLAKALFAQRSQESQRLAAAYMRESGPIYVVN
                     NVRYVIYLDQGSSAQAPAHFVEKGIRLAIDATAREVSK"
     gene            complement(3525..3962)
                     /locus_tag="VFG22_16940"
     CDS             complement(3525..3962)
                     /locus_tag="VFG22_16940"
                     /inference="COORDINATES: protein motif:HMM:NF024943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator-like protein"
                     /protein_id="HET6417983.1"
                     /translation="MTTQTEARDAVMDAIRDAWLADALTMGIALQWDNVKADPLGEDD
                     DGNPLPWARVSVRHIFSGQETIAGPGSRKHQTEGVVTVQVFTPSGDGHSLADQIVEVL
                     KPALRNVAIGNLWFYNVRVNELGTDGAWFVQNFLANFRYSELA"
     gene            complement(3979..4404)
                     /locus_tag="VFG22_16945"
     CDS             complement(3979..4404)
                     /locus_tag="VFG22_16945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417984.1"
                     /translation="MANFTKLAETAQRLVEENGRSVTLFRKSRTPANASEPWRGPDLT
                     TPDPAAGIGPVLVCFVPASGSGFGKVLFDEDPTIRVKIDQVGLLAANSIEELTPPFTA
                     ADVEQCDTLRDGAQVYKIKSVGHLKPATKSMLFVLGLSS"
     gene            complement(4414..5325)
                     /locus_tag="VFG22_16950"
     CDS             complement(4414..5325)
                     /locus_tag="VFG22_16950"
                     /inference="COORDINATES: protein motif:HMM:NF042800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding protein"
                     /protein_id="HET6417985.1"
                     /translation="MTVVVESGSGVIGANAYANAAYVTTYLTTRSRETAWSALTSAQR
                     DSYVVAATDYIEKRWGHRFLGIRQYAFDDVPAIASIVFADAPVADEVLAINDFTYTYK
                     AALSTDSPQGNNFEVLLGSAGADSASNLYDALVASADNAGSTFQTGTVANRHVIGVTL
                     ETATIALTSVAPGESGNYNTLTGPLTNVTLTTWAGGIDGGSQALSFPRLGLYNWSGRK
                     VEGVPLKLKQAMAEYADRVRTATLDPDPVFDDQGGSITKLREKVGPIEVETEYSDGTH
                     GRVLIRPYPAADRLLDEYVQPAGVIRA"
BASE COUNT         1004 a         1831 c         1698 g         1088 t
ORIGIN      
        1 aatgcgcagc cggttttgca ggttcgtgag ctggtcggca aatccaacaa gctcacgcac
       61 gcccagggag acgccgagca cggcgaatgc gcggcggagt gtggagccga ggctgctcgc
      121 gcggctctct gcacgttgca ggctcgtgtc gatctggtcg atgggtttcg tgttgacgcg
      181 cgagcctacc tgcgagattt cctgcttgag atcgcgcgca gacccttcgg agcgagttag
      241 atttgcgttg agttgcttca gctcgccaat gacaccagcg tcgcgagcct gaagagctcc
      301 gacaatcgcc tgcctgagct gcgccgcctc cttctcgacg gaatccaggg acttctcgac
      361 cgtcttcagc cccttttggg ccttgccggg atccacgacg acgtcgatgc ggaagtcagt
      421 catcggcgcc tacccgctcg catgctgccg ctcatcttgc cggtggtctt ctgcgacgcc
      481 tcgcgcttcg cctggcgctc ctgcttcttg gcctccttct cgccctccga cgtcaggtgc
      541 tcgcggtact gagcatcgag cgagagaatc acgttggcga agaagttgca catggggcgt
      601 ccgtagcctt gacggtccgc gaagtcccac gccttcgacc acgggattgg catcatgcca
      661 cccatcgagg ccggcctctc ggtcgtgagc agacaatagg cgttgaccca gaacgcatcc
      721 gcacgaccct ccctcggtct ctgcgtgtac cagtcaggca gccccccgcg tgtagagcgc
      781 tggtactggc ccgtctgaat ggcccacccg tcacggtccc agcgctcttt ccagaggagc
      841 cgccacttca gttttttgcc agccctcgcg ggtcgagttc ggactcggag cccgagccgt
      901 tgaaggtctt ggtattgagg cagaacgcgc gcacccggtt gaacaggtac ttcgggatct
      961 gctgaaaaaa cgccagactc gcctcaagcg tgaacggcac ctgagcgttg ctcccgcgct
     1021 gtcgaatgcc gccccagccc ttcacgatcg tgcgtgcgta gcgctcgcgc tcgaaggcaa
     1081 gctcccgcgc atccttctcg accggatcct cgtcgtcctc acctactttc agcgattcga
     1141 gcgccgactg acggatcagc atctcctcgt actgaggatt gtcatgcgtc gacaccagga
     1201 gtaggtaggc atccgggctc accatcggca tctccagccg ccgcaccttg cccgcaacct
     1261 ccagctcgtc gagcccgaag tcgaagtgcc cattcgagcc aaccgcggtg cgccgaggct
     1321 tccttttcgt tgcagtcatg ttgcctgtca ctcctcaggt ggtgtggatc tcaaatcagg
     1381 tcagccgcca tcaacccaga aggactcagc tcgaaagaac tccgggcacc gcggggaaga
     1441 gcgagattcc catggaatat ccgaaggtgt cggacgtgaa cgcttgcagc acgaggttca
     1501 cgagcaccga ttgatcgagc ggatactcgc gcccgccacc ggatgccgtg gcctccggga
     1561 tgtcgtaggc gatggccccg tcctcgttgc ggaagatcga cgcgaaagtc acggtcgtgt
     1621 tgttcttgat ggcgttgatg atgctcgcac tggtgaagag catttgcccc tcgacgtcga
     1681 cctcgaatag cccggcgttc acgaaggtgg cacccagcgt cccgaggcag tattcgggcg
     1741 agacgttgtt gcgcgccgtg agcgtcagcg acttgaaaca gacgtcggac accgaggaga
     1801 tcaggtccgt ggtcaggctc tgaacgtcgc tcgacgtatt gaaggccgtg ttgcgcagcg
     1861 gagacttcgc cgtggaagcc ccagtcttgc gcgtgcccgt gatgtcgtcg gcgttggtgc
     1921 cgatgaagcc gaagttgacg gtcgacttgt cctgaagcgg gaagttgaac gacagctcgt
     1981 tggcgaaatt accgatggcg tactcgtact ggtcggtgcc gatgccgccg aggtcgggga
     2041 acaccgcctc aaactggaac gtgcgctcga gatagcgcgt gtcgtcggaa tccgccgtca
     2101 ccgcgacgtc acgcaggaag ttcccgaaca tgatgtcgat cgtctcgggg ctgaatgggc
     2161 tgctaccaga agctgccgcg atgatcgggt cgatcttgtc gcaggagagc acggtagccg
     2221 tgatggccgt gatgcgagcg tagccgtaga cgtcatccgt gccgccgtcg tcgtaagcgt
     2281 tctggacgac gccgctcacg tccatgcttc cgatgtggat ttcctgaccg accgtgagtc
     2341 cgatcgtcgt ccagttgagg tccgcggcgc tggtgagcgt gagcgtcgag cccgagatcg
     2401 ccatcgtcag atcatcggtg cggatgccgc agacctccag ggtggcgttt gatggcggcg
     2461 tctcgtcgac gaggacggtg gacacgtcca ccgaggtatt ggtcccggca acgtcggcgg
     2521 cgagcacgtg aagcccgttg ttcaccgcgt tcgtgtagcc cttggcgaac agcagggttt
     2581 ggacggtggc ggcgccgtac tgcattttgc cggccagcag cgtggacgcg gcgtcgatgg
     2641 tgaacactcc gcccgcggcg actgcgggag gcggaacagt gcccgtggtg gccctgagat
     2701 tgaactcgac gttcgtgaac tccgagaaca tgaagccctc ggcgaacagc cggaacgctt
     2761 cgaatgtcag gtcagtgtcg tactcgaccg aactctccag gttggtgacg gtgcccttct
     2821 tgcgcccgcg gtcctgactg atggggcgac gcaccgtggt cgtgagcacc gcgccatagg
     2881 cgccgatcgt gtcgaactcg actacagtcc actgcggcgt ccccggcagc acgccgatgc
     2941 tcgtctctgt cgcaacccgc agagacgtat tgttggtcaa tacgcgcgcc atgcgtctcc
     3001 ccccatcgat ttcggaagcg tcacttcacc atcgatgcga gattagcaag attgcgaacg
     3061 ccgtggcttg ccagaaacgc gaaatcttgg acaagctgcg ctacttggac acctcgcgcg
     3121 ccgtagcgtc gatggcgagg cggattccct tctcgacgaa gtgcgcggga gcctgagcgc
     3181 tcgatccctg gtccaggtag atcacatagc gcacgttgtt cacgacgtag atcggtcctg
     3241 actcgcgcat gtaggctgcc gcaagccgct gcgattcctg cgatctttgc gcaaagagcg
     3301 ctttggctag ctggatggca gccgatcggt cagacggctg atccgccggc cccgagcggt
     3361 caggggcgcc cgtgctaggc gtccatccgc cgcgcgcaaa tccagtgtcc accggcgtcg
     3421 gcgtcgaaag cgactggtag agccgcagtt gcaatcgtcg catgacccgg ttgatcgagt
     3481 cccctaggtc cccgacgacg atacgggtct ggttgcgcgc catgtcaggc aagctcggag
     3541 tatcggaagt tcgcgaggaa gttctgcaca aaccacgcgc cgtctgtgcc cagctcgttc
     3601 acccgcacgt tatagaacca gagattcccg atcgccacgt tccgcaaggc cggtttcagc
     3661 acctcgacaa tctggtcagc cagcgagtgc ccgtcgcccg atggggtgaa cacctgcacg
     3721 gtcacgacgc cctcggtctg gtgcttccgg ctcccaggac cagcaatcgt ctcctgcccc
     3781 gagaagatgt gtcgcaccga cacccgcgcc catggcagcg gattcccgtc gtcgtcttcg
     3841 cccaaggggt cagctttgac gttgtcccac tggagcgcga tccccatggt cagcgcgtcc
     3901 gcgagccagg cgtcgcgaat cgcgtccatg accgcgtctc gagcttcggt ctgggttgtc
     3961 atgcttcccc tccagcgctc aagaactcag ccccaggaca aacagcatcg acttggttgc
     4021 aggcttcagg tgccccaccg acttgatctt gtagacctga gcgccatcac gcagagtatc
     4081 gcactgctcg acatcggcag ccgtgaaggg cggagtaagc tcctcgatgg agttggcagc
     4141 cagcagcccc acctggtcga tcttcacccg gatcgtcggg tcctcatcga acagcacctt
     4201 cccgaatccg ctgccagaag ccggcacgaa gcagaccagc acgggaccaa tgccagccgc
     4261 ggggtccggg gtcgtgagat ccggcccgcg ccacggctcg gaggcgttcg caggcgtccg
     4321 gctcttgcgg aagagcgtca cgctgcgacc attctcctcg accagccttt gcgctgtttc
     4381 cgctagcttg gtgaagttcg ccatggtcgc tccttacgct cgtatcacgc ccgctggctg
     4441 cacatactcg tcgagcagcc gatccgccgc cggatagggc cggatcaaca cgcgcccgtg
     4501 cgtgccatcg ctgtactcgg tttcgacctc gatcggaccc accttctcgc gcagcttcgt
     4561 gatggagccg ccttggtcat cgaacaccgg gtccggatcc agggtcgccg tgcgcacgcg
     4621 atccgcgtac tccgccatcg cctgcttgag cttgagcggc acgccctcga ccttgcgccc
     4681 cgaccagttg tagagcccga gccgcgggaa gctgagcgcc tgactgccgc catcgatgcc
     4741 cccggcccag gtggtcagcg tcacgttcgt gagcggcccc gtcagcgtgt tgtagttgcc
     4801 gctctcccca ggcgccaccg acgtgagcgc gatagttgcc gtctccagcg tcaccccaat
     4861 cacatgccgg ttcgccaccg tgcccgtttg aaacgtcgag cccgcgttgt cagcgctcgc
     4921 caccagcgca tcgtagagat tcgacgcaga gtcagcaccc gcagacccaa gcagcacctc
     4981 gaagttattc ccctgtggag agtcggtaga gagcgccgcc ttgtaggtgt aagtgaaatc
     5041 gttgatcgcc agcacctcat ccgcaaccgg agcgtccgcg aacacgatcg acgcaatcgc
     5101 cggcacgtcg tcaaaggcat actgccggat cccaaggaac cgatgccccc agcgcttctc
     5161 gatgtagtca gtagcggcga ccacgtagga gtctcgctgc gccgaggtca gtgctgacca
     5221 cgccgtctcg cgcgagcgcg tcgtgagata cgtggtcacg tatgccgcgt tcgcgtaggc
     5281 gttggcgccg attactcccg atccagattc cacgaccacg gtcatcggta gattcctcgg
     5341 ctcaaaacac cctcaccact caaacaagat gcacgccaca gcaaagagaa tcaccaccaa
     5401 aatcaccagc acaacctcag atccataccc gccccttcgg cgcgtcatgg gttcgttcgc
     5461 gtcagcccat cggcggagat cacgatgctt tcaatcaccg tggtctgatc gtcgttgtag
     5521 aagtctacgg ttttctgtcc gactccaggg gtgtcttccg atgtcaccgc cttattcaga
     5581 agcaccttgc gtgcgaggtc cggccccacg atggcggaat a
//
LOCUS       DASRZF010000381         2868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4759659, whole genome shotgun sequence.
ACCESSION   DASRZF010000381 DASRZF010000000
VERSION     DASRZF010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4759659"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="VFG22_16955"
     CDS             <1..860
                     /locus_tag="VFG22_16955"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET6417986.1"
                     /translation="RCQVGARGIGVMLGLQATFHPFMGFPSYKAISQVPLAERVKAMR
                     DPELKARMIAEKSEPVAGDGSMLPPLADYFLSNLEMVAMRLFRLGEKPNYEPSPTESF
                     GAAAMSRGEPVLSLIYDAMLEQDGEALLYFPVYNYTGMNLDAVHEMLTHPLALPGLSD
                     GGAHVGTICDASFTTFLMTHWARDRDHGRIPLERIIQMQANDTARFLGLSDRGLLAPG
                     RRADINIIDFDNLELLHPTMVKDLPAGGQRLMQHARGYVATLVNGEAIVENGKLTGTR
                     PGRLVRAGR"
     gene            869..1783
                     /locus_tag="VFG22_16960"
     CDS             869..1783
                     /locus_tag="VFG22_16960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-PLC domain-containing protein"
                     /protein_id="HET6417987.1"
                     /translation="MGKLGNCALTMLSALLLLSCGTEGESLVPDAGLDAGAQPDGGEP
                     DPKCNGHEELCLRRFDDVAFPMTHNSMSNAEAGWILPNQNFGITRQLNDGIRGLMLDT
                     YDEDGELLLCHSVCLAGSQPLVEGLAEIRSFLKANPEEVVAIIFENYITHAQTAGAFE
                     ESGLIDFVYAHEIGEPWPTLGTLIDAGTRLVVFQEKQPEDDAFPWLMNIWDHAWETPF
                     SFSAPEDFVCTPNRGNPANPLFLLNHFLTATLGGRPEFAEMVNYNPLFIDRAVQCDEE
                     GGALPNFVAVDFYDIGDLFEVVDTMNGF"
     gene            complement(1920..>2868)
                     /locus_tag="VFG22_16965"
     CDS             complement(1920..>2868)
                     /locus_tag="VFG22_16965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417988.1"
                     /translation="NWGDELDLALETEFWDLIGNSDHVHYPAWHSKAKAYVTANLNAD
                     PRLSMLISAGAVFSLTTLFQYDDPLAHLAEYSDYIADATVGIHDAIEPLGDHEPALVF
                     FHNNQAMLAFLLSENADLGICHLEKLRNLEDSNPTFHGTEGRAAAPFTYGMVDDPAYV
                     DYAIAMMEGCNNWICNWTSKLAPFKPIGQLMTLAELYAIQGSTAADSGVRAAAYDEMN
                     SLLDRAALLGTARGYPFMDRIESLRTELPTREYTDGIGLGRSPMPIYSNDSNCSNCHI
                     GGLSGRYGEDLDDPYPGAGSWSAKPGPPAFKSELPMSCD"
BASE COUNT          590 a          849 c          871 g          558 t
ORIGIN      
        1 tccgctgcca ggtcggcgcg cgaggaatcg gtgtgatgct cggacttcaa gcgaccttcc
       61 accctttcat gggctttccg agctacaagg cgatcagcca ggttccgctg gccgagcggg
      121 tgaaggccat gcgcgaccca gagctcaagg cccggatgat cgccgagaag agcgaacccg
      181 tggcgggcga tgggagcatg cttccgccgc tcgcagacta cttcctttcg aatctcgaga
      241 tggtggcgat gaggctcttc cggctcggag agaagcccaa ctacgaaccc tcgccaaccg
      301 aaagcttcgg ggctgcggca atgagccgag gcgagcccgt cctgagcctg atctacgatg
      361 cgatgctcga gcaggacggc gaggcgctct tgtacttccc cgtctacaac tacacgggga
      421 tgaatctcga tgcagtgcac gagatgctga cccacccgct cgccctgccc ggcttgagcg
      481 acggcggcgc acatgtcggc accatttgtg acgcgagctt cacgactttc ctgatgacgc
      541 actgggcgcg agatcgggat cacggtcgca ttccgctcga acgcatcatt caaatgcagg
      601 ccaacgacac ggcccgattc ctagggcttt cggatcgggg ccttctagcg ccaggacgac
      661 gcgcggacat caatatcatc gacttcgaca acctcgagct cttgcacccc accatggtca
      721 aggacctgcc cgcaggaggt cagcgtttga tgcagcacgc aaggggctac gttgcaacgc
      781 tcgtcaatgg tgaagcgatc gtcgaaaacg ggaagctcac gggcacgcga ccggggcgcc
      841 tcgtgcgggc ggggcgatga ggagcgccat gggtaagtta ggcaactgcg cgctcacgat
      901 gctctcggct ttgcttctcc tttcgtgtgg aacggagggc gagagcctgg tccccgatgc
      961 cggactcgat gccggcgctc agcccgacgg gggcgagccg gatcccaagt gcaacggtca
     1021 cgaagagctc tgcctcaggc ggttcgacga tgtcgccttt ccgatgacgc acaactcgat
     1081 gtcgaatgcg gaggctggct ggattttgcc caaccagaat tttggaatca cgcggcagct
     1141 gaacgacggc atacgcgggc tcatgctcga cacctacgac gaggacggtg agctgctcct
     1201 ctgtcattcg gtctgtcttg ccggaagcca acctctggtc gaagggctcg cggagatccg
     1261 cagcttcctc aaagccaatc cggaggaagt cgtcgcgatc attttcgaga actacatcac
     1321 gcacgcgcaa accgcaggcg cattcgagga gagcgggctg atcgacttcg tctatgccca
     1381 cgaaatcggc gaaccatggc cgactctcgg cacactgatc gatgccggca ctcggctcgt
     1441 ggtgtttcag gagaagcaac ctgaggacga cgccttcccc tggctgatga acatctggga
     1501 tcatgcgtgg gagacaccct tctccttttc ggctcctgaa gatttcgtct gtacgcccaa
     1561 tcgaggaaat ccagccaacc cgctttttct cctcaatcat ttcctcacgg cgaccctggg
     1621 cggaagaccc gaattcgcgg aaatggtcaa ctacaacccg cttttcatcg atcgcgcggt
     1681 ccagtgcgat gaagaagggg gggcgcttcc gaacttcgtc gcagtcgatt tctacgacat
     1741 cggtgacctt ttcgaggtcg tcgacacgat gaacggtttc tagggtgcag aggatgcaat
     1801 gggcaaatcc gaaaaccgac tggcgatgac gatgcagtgc aaggtagcgg atgaaaccgg
     1861 cagcgagccg gtccgctgcg agatcatttg ggcctgggtc ccgaagaagc gcgactagct
     1921 cagtcgcagc tcatggggag ctcggacttg aaggcaggcg gccctggctt ggcggaccag
     1981 cttccggcgc ccgggtaagg gtcgtcgaga tcttcaccgt accggcccga aagcccgccg
     2041 atgtgacagt tgctgcaatt gctatcgttg gaataaatag gcattggtga acgcccaagt
     2101 ccaatcccgt ccgtatactc gcgagtcggg agctcggtgc gaagtgactc gatcctgtcc
     2161 atgaatgggt agccgcgcgc agtcccaagc aacgctgcac gatccagcaa ggaattcatc
     2221 tcgtcatatg cggccgctcg cacccctgaa tcagccgccg tcgacccctg aatcgcgtat
     2281 agctcggcca gcgtcatgag ttgcccgatc ggtttgaacg gcgccaactt ggatgtccag
     2341 ttgcaaatcc aattgttgca gccttccatc atggcgatcg cgtaatcgac gtatgcagga
     2401 tcgtccacca tgccataggt aaacggcgcg gctgcccttc cctcggtgcc atgaaacgtc
     2461 ggattgctgt cctcgaggtt gcggagcttc tcgaggtggc agatcccgag gtccgcattc
     2521 tcgctcaaga ggaacgccag cattgcctgg ttgttgtgaa agaagacgag cgcgggctcg
     2581 tggtcgccga ggggctcaat cgcgtcgtga atgcctaccg tcgcatcggc gatgtaatcg
     2641 gagtactcgg ccaggtgcgc cagcggatcg tcgtattgaa agagcgtagt aagactgaag
     2701 acggcgcccg ccgagatcag catgctaaga cgtgggtcgg cgttgaggtt tgcagtgaca
     2761 tacgccttgg ctttcgagtg ccaagcgggg tagtgaacat gatcggagtt tccaatcaaa
     2821 tcccagaact cggtttcaag cgcgagatcg agctcatccc cccaattg
//
LOCUS       DASRZF010000382         4945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_634148, whole genome shotgun sequence.
ACCESSION   DASRZF010000382 DASRZF010000000
VERSION     DASRZF010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 4945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4945
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634148"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VFG22_16970"
     CDS             <1..201
                     /locus_tag="VFG22_16970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417989.1"
                     /translation="ASPAVGLRITDGYRDNVTAIIKELCEASRKHDVEIDVLLIDGHD
                     MLRKAREKAVVFYPWKPKPFGR"
     gene            248..1543
                     /locus_tag="VFG22_16975"
     CDS             248..1543
                     /locus_tag="VFG22_16975"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HET6417990.1"
                     /translation="MPAVRTPVPAAVGRAWVERLAGSECPALTTRRKRREEQTGANHD
                     PIVWVEAEGANVIDADGNRYVDLTSGFGVAFVGHRHPDVVRAVKRQADRLLHALGDLH
                     PSDVKIELLERLSALSPWPQARVMLSLSGADAVTAALKTAVMATGKTGVVAFEGSYHG
                     LTYGPLAVSGFAERFRQPFQAQLQRDVQFMPWPRHDTSLDEALQALPRDWTGIGAVIA
                     EPIQGRAGVRLPPAGFLAALALACRQNGALLIVDEIFTGMGRCGAWWRSVDEDVTPDV
                     ICIGKALGGGLPVSACLASEEVMGAWGTAEREAIHTGTFYGDPVGAAAALATIDLIER
                     EKLCERAAERGDSLAETLRSAGLSEVREVRHAGLMIGLQLERPMQALTVTRTLLERGY
                     LVLPAGAQADVLQLAPPVMIADAQLDGFIQALKATLEAG"
     gene            1540..2604
                     /locus_tag="VFG22_16980"
     CDS             1540..2604
                     /locus_tag="VFG22_16980"
                     /inference="COORDINATES: protein motif:HMM:NF016338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-protein synthetase"
                     /protein_id="HET6417991.1"
                     /translation="MSTRSRLKGRIQAMLSDFESGIGVDPAVRDELIEDLAEWQSQEI
                     PEYGRLQRLGPWPPALPTDVFRFRRIASQPAHEDVRVFRSSGTTASERSVHAFRDLSL
                     YDFAARVAARHMLFPDIERLRLVVLAPHEDEAPNASLEYMLARFAEWFGTDSIWAWSS
                     GALDLELLSGALRSAEASEEPIAVLGTSFAFVHAEDGLGDQVFSLPPGSRVMQTGGYK
                     GRSREVDKQVLLEAIGARYGVPAPWIINEFGATELSSQLYETTLREDIYGGPGPRRLW
                     VPPWVRATPVDPETLEPVEGNAVGILRIDDAANLDSVCCIQTADLAQRLADGIVVLGR
                     APGATPRGCSLAADQALGAR"
     gene            2601..3812
                     /locus_tag="VFG22_16985"
     CDS             2601..3812
                     /locus_tag="VFG22_16985"
                     /inference="COORDINATES: protein motif:HMM:NF017686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HET6417992.1"
                     /translation="MTQDAEAIRARAEDVAQAGVWLRSVSTKERVHWLSKASSTLARR
                     AEDACTALSEATGLSAPMVRWATRTTLDTIGEETLLELAAQASKRSSPSSKPIAMLSL
                     VLAGNVFTASVRGILVPLLLGVPVLAKVSSKDSLFPAMLLDAIRDSDPRLGASLDLVT
                     FTGGDAALEAAFVERADAVSAYGSDETIAAMALRLGAKPLIAHGHGVSVAYCDAEALH
                     ARAIDETIERLALDISAYDQRGCLSPQLVYVEETRHMTALSFARRLCEEGLGPMGLSL
                     PRGALPIATGAVQAQWRGLAEVEGDLLRGDAYAVAVRPSHPIRWSPGYRNVSLSPVSG
                     IDDALHAMKPIGSNLKCIGASLNSVSSLRDRLAQSRALGAYVCALGEMQIPALDAPAD
                     GRPIWYGLFRP"
     gene            complement(3831..4568)
                     /locus_tag="VFG22_16990"
     CDS             complement(3831..4568)
                     /locus_tag="VFG22_16990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET6417993.1"
                     /translation="MRLGIFSDVHANLEAMSAVMEAYGSEDIDVYYCLGDVVGYGGSP
                     NECSDIVRDVAEVTILGNHDAAVAGRMDYSYYYEAAREALDLHARELTPENMAWLKQL
                     PYKHCLKGIGVDLCHGSPVRLEEFEYIFAPDQAAECLPIWNELGHITFIGHSHLCKVF
                     ALRPGEVEELPPEDFTLQDGTKYIVSVGSVGQPRDYDNRASYTVYDSDSRSFHFKRIE
                     YDIEGAASKIFGARLERNFGQRLFIGV"
     gene            complement(4583..>4945)
                     /locus_tag="VFG22_16995"
     CDS             complement(4583..>4945)
                     /locus_tag="VFG22_16995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417994.1"
                     /translation="LRLLEAWSSPARIEEALSILSVPRTVFELSRRRIVADALRFGLA
                     MSDALVVQAVQFGFASEAKDLVHALGCKFEQIVPLASVQGLDEGQVRANRRALERLAM
                     MHGTSTELALSCTMEHSG"
BASE COUNT          920 a         1535 c         1577 g          913 t
ORIGIN      
        1 gcaagccctg cggtcggcct tcgaatcacc gacgggtacc gggacaacgt caccgcgatc
       61 atcaaggagc tctgtgaagc cagcagaaag cacgacgtag aaatcgacgt gctcctcatc
      121 gatggtcacg acatgctccg caaggcgcgc gagaaggcgg tcgtgttcta tccctggaag
      181 cccaagccct ttgggcgctg agctttcgga cgcatgctag aaggtccggg tgaccttcgg
      241 tgatgcaatg ccagccgtac gaacgccggt tccagccgcc gtgggcaggg cttgggtaga
      301 gcggcttgcg ggaagcgagt gccctgcgct aaccacgcgc cgcaaacggc gtgaagagca
      361 aaccggggcc aaccacgatc cgatcgtctg ggtagaagcc gagggcgcga acgtgatcga
      421 tgcagacggg aaccgatacg tcgatctcac ttcggggttc ggtgtcgcct tcgtaggaca
      481 tcgtcatccc gatgtcgtcc gggcggtgaa gcggcaggct gatcgattgc tacatgcgct
      541 tggcgatctg catccctcgg acgtgaagat cgagcttctc gagcgccttt cggccctctc
      601 gccatggccc caggcgcgcg tgatgctctc gttgagtggg gcagacgcag tcaccgccgc
      661 gctcaagacc gccgtcatgg ccacgggcaa gacaggcgtg gtcgcattcg agggcagcta
      721 ccacgggcta acctacggtc cgctcgccgt gtcgggattc gcggagcgtt ttcggcaacc
      781 gtttcaagcg caactgcagc gagacgtgca gttcatgccc tggccgcgac acgacaccag
      841 cctggacgaa gccctccaag cgctgccgcg agattggacc ggcatcggtg cggtgatcgc
      901 ggaaccgatt caagggcgcg caggcgttcg cttgccgccg gctggttttc tcgcggcgct
      961 cgcactggcc tgcaggcaga acggggcgct gttgatcgtg gatgagattt tcaccggtat
     1021 ggggcgctgc ggcgcctggt ggagatctgt ggacgaagat gtgactccag acgtgatctg
     1081 catcggcaaa gcactcggcg gcggactgcc cgtgagcgct tgcctcgcga gcgaagaagt
     1141 gatgggggcc tggggaacag cggagcggga ggcgattcac actgggactt tttacggaga
     1201 tccggtgggc gcggctgcag cgctcgccac aatcgatctg atcgaacgcg agaaactgtg
     1261 cgaacgcgct gccgaacgcg gcgactcgct ggcggaaacg ctgcgctccg ccggcctttc
     1321 ggaagttcga gaagtccgcc acgctggcct gatgatcggc cttcagctgg agcggccaat
     1381 gcaagcgctc accgtaacgc gaacgctgct cgagcgagga tacctggtct tgccagcagg
     1441 cgcgcaagcc gatgtgcttc agctcgctcc accggtgatg atcgccgacg ctcagctcga
     1501 tggcttcatc caagcgctga aggccacgct ggaggcgggg tgagcactcg atctcggttg
     1561 aagggacgca tccaagccat gctctccgac ttcgaaagcg ggatcggcgt cgaccctgca
     1621 gtccgcgacg agctgatcga agacctcgcc gagtggcagt cgcaagagat tcccgaatat
     1681 ggtcgattgc aacggctggg cccctggccg ccagcccttc cgacggatgt attccgattt
     1741 cgacgcattg cgagccagcc cgcacacgaa gacgtgcgtg tcttccgaag ctcgggcacg
     1801 acggcctccg agcggagcgt gcacgcgttc cgcgatctga gcttgtacga tttcgccgcg
     1861 cgcgttgctg cccgccacat gcttttcccc gacatcgaaa gactgcggct ggtggtgctc
     1921 gcgccgcatg aagatgaagc gccaaatgcc tcactcgagt acatgctcgc gcgatttgcg
     1981 gagtggtttg gaacagactc gatttgggcc tggagtagcg gcgcactcga cctcgagctg
     2041 ctcagcgggg cgctccgaag cgcggaagcg tcggaagaac cgatagcggt gctcgggacc
     2101 tcgttcgctt tcgtgcacgc cgaggatggg cttggagacc aggtcttttc actcccgcct
     2161 gggagccggg tgatgcaaac cggcggctac aaaggccgct cacgcgaagt ggacaagcag
     2221 gtcttgctgg aagcgattgg tgcgcgctac ggggtgcctg ctccatggat catcaacgag
     2281 ttcggtgcga ccgagttgag ctcacagctg tacgagacga cgcttcgcga ggacatctac
     2341 ggtggcccgg ggccgcggcg tctctgggtt ccgccttggg ttcgcgctac gccggtcgac
     2401 cccgagaccc tcgaaccggt cgaagggaat gccgtcggca ttcttcgaat cgacgacgcg
     2461 gcgaatctgg attcggtctg ctgcatccag accgcagacc tcgcgcagcg cctcgccgat
     2521 ggcatcgtcg tgctcggccg cgcaccggga gcgacgccgc ggggctgttc acttgccgcc
     2581 gatcaggccc tgggggccag atgacgcagg atgcagaagc gattcgcgct cgggcagagg
     2641 acgtcgccca agcgggcgtc tggctgcgct cggtgagcac gaaggagcgt gtgcactggc
     2701 tttcaaaagc gtcctccact ctggcccgac gagccgaaga cgcgtgcacc gcgctttcgg
     2761 aagcaactgg attgagcgcc cccatggtcc gctgggcaac acgaaccacg ctcgacacga
     2821 tcggcgaaga aacgctgctc gagttggccg ctcaggcatc caagaggagc tccccgtctt
     2881 cgaaaccgat cgccatgttg tcgctggtgc tggccggcaa cgtcttcacg gcctcggtgc
     2941 gcggcattct cgttccactc ctcctgggtg ttcccgttct cgccaaagtt tcatcgaaag
     3001 acagcctgtt cccagcgatg ctcttagacg cgatccgtga cagtgatcca cgattgggcg
     3061 cgtctctcga cctggtcacc ttcaccggcg gtgacgcggc attggaagcc gcattcgtcg
     3121 agcgggctga cgcggtatcg gcgtacggca gcgacgagac gatcgcggcg atggcgctgc
     3181 ggctcggcgc caagcccttg atcgcccacg gacacggggt aagtgttgcg tattgcgacg
     3241 ccgaagcgct gcacgccaga gcgatcgacg aaaccatcga gcgactcgct ctcgacatct
     3301 cggcctacga ccagcgcggc tgcctttctc cccaactggt ctacgtcgag gagacgcggc
     3361 acatgaccgc cctgagcttc gcgagacgtc tgtgcgagga gggcctcggc cccatgggtc
     3421 tctccttgcc gcgaggcgct ttgcccatcg cgaccggtgc ggtgcaggcg cagtggagag
     3481 gccttgccga ggtcgaaggc gacctgctgc gcggagatgc ctacgcggtc gccgttcgcc
     3541 ccagtcatcc gattcgatgg agcccaggct atcgaaacgt aagcctttcg ccggtatccg
     3601 gcatcgacga cgcgttgcat gccatgaagc cgatcggctc gaacctgaag tgcatcggtg
     3661 ccagcctcaa ttcggtctcc tcgcttcgtg accgccttgc tcaaagccgg gcgttgggcg
     3721 cctacgtttg cgctctcggg gagatgcaga ttccagcgct cgacgcgccc gcagatggcc
     3781 gaccgatttg gtacggcctc tttcgcccgt gagcgttagg acgccatcga ctagacgccg
     3841 atgaaaagtc gctggccgaa gttccgctcc agccttgcac cgaaaatctt cgacgccgcg
     3901 ccctcgatgt cgtattcgat gcgcttgaag tgaaacgagc gagagtcgct gtcatacacc
     3961 gtgtaacttg cgcgattatc atagtcgcgt ggttgaccaa ccgagcccac gctcacgatg
     4021 tacttggtgc cgtcctgcag agtgaagtct tcgggaggca gctcttcgac ctcgccgggg
     4081 cgaagcgcga agactttgca caagtgggag tgaccgatga atgtaatgtg accgagctcg
     4141 ttccagatcg gcaagcactc ggcagcctga tccggtgcga agatgtattc aaactcctcg
     4201 agccggaccg gcgacccatg acataggtcc acgccaatgc ccttgaggca atgcttgtac
     4261 gggagctgct tgagccaggc catgttctcc ggcgtgagct cgcgcgcgtg aagatcgagc
     4321 gcttccctgg cagcctcgta gtagtagctg tagtccattc gaccggcgac ggctgcatcg
     4381 tggtttccga ggatcgtgac ttcggctaca tcacgaacga tgtccgaaca ctcgttcggg
     4441 ctgccgccat agcccaccac gtcgcccaga cagtaataga catcgatgtc ctcgctgcca
     4501 taggcttcca tgacagcaga catggcctcc aaattggcgt gaacgtcgct aaatataccg
     4561 agccgcatga ttggttcgcc agttaccccg aatgctccat ggtacacgaa agggccagtt
     4621 cggtcgaagt accgtgcatc atagccagtc gctccagtgc tcggcgattt gcgcgaactt
     4681 gcccttcgtc gagtccttgt acggacgcca gaggcacgat ttgctcgaac ttgcagccaa
     4741 gggcgtgcac gaggtccttc gcttcagagg cgaatccaaa ctgcaccgcc tgcacgacca
     4801 gagcatccga catggcgagg ccgaaacgca atgcatcggc aacgatccgg cgcctggaga
     4861 gctcgaagac tgtcctcggc acgctcaaga tactgagtgc ctcttcgatg cgcgcggggc
     4921 tgctccaggc ttcgagtagt cgaag
//
LOCUS       DASRZF010000383        11310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2016891, whole genome shotgun sequence.
ACCESSION   DASRZF010000383 DASRZF010000000
VERSION     DASRZF010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 11310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11310
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2016891"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VFG22_17000"
     CDS             <1..258
                     /locus_tag="VFG22_17000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET6417995.1"
                     /translation="DWFGEMSIIDVQPRSASVRAVAPTLVLSVTAEHVENLLYRRDVK
                     DYALFIMNIARELSRRLRVADGILAQFYAALSHRYGAPPKP"
     gene            complement(283..1140)
                     /locus_tag="VFG22_17005"
     CDS             complement(283..1140)
                     /locus_tag="VFG22_17005"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HET6417996.1"
                     /translation="MASTDAKVIYVVSDSTGETAERVTRATLLQFPNHRIRLKIERRV
                     RDRRALTAILESAAAQEAMVVFTLVRPELRDHFNEVASRLQVRHVDVIASLIHHVGHY
                     LEADPMNIPTAELPLSPEYFRRVEALEFAVKSDDGKEPRNLDKADLVLVGVSRTSKTP
                     LSTYLAGRGLKVANVPIVLGVALPKEVYELPGYRVVGLTIDVDQLRDIRKQRLQQLGM
                     PADANYGLRDHVKAELEYAHSILRENPEWMVVDVTNCAIEETATIILEALKDREVLKG
                     LTRPPPLVF"
     gene            complement(1144..1278)
                     /locus_tag="VFG22_17010"
     CDS             complement(1144..1278)
                     /locus_tag="VFG22_17010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6417997.1"
                     /translation="MYLNIVNSDRSRKRNKAARRKAQRKAKNRRRVNRMHGRKLGRRG
                     "
     gene            complement(1362..2435)
                     /locus_tag="VFG22_17015"
     CDS             complement(1362..2435)
                     /locus_tag="VFG22_17015"
                     /inference="COORDINATES: protein motif:HMM:NF039588.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HET6417998.1"
                     /translation="MAKTNPGNFFEDFEVGAELVHAVPRTITEGEAALYIGLTGSRYP
                     LHCSAEFARSLGYPRETINDMLVFHIVFGKTVNDVSLNAVANLGYAGVRFLTPVYAGD
                     TLRSISKVIGKKENSNGKTGVVWVKTVGTNQRGEDVLEYFRWVMVNKRDPSMPTGAAD
                     RPAMPKEVTPAELLVPKDLDLSAYEDWPSGGNAYFEDYEPGEKIDHVDGMTIEESEHA
                     IATRLYQNTAKVHFDAVQTKDTRFGRRLMYGGHVISIARSLSFNGMEKVLGILAWNGG
                     AHANPTFAGDTIYAYSEILDKADLPGRSDCGALRVRLVGLKDVRPAEAGFPLKRSKDG
                     REVYEKSVVLDLDYWLLIPRRKR"
     gene            2598..3227
                     /locus_tag="VFG22_17020"
     CDS             2598..3227
                     /locus_tag="VFG22_17020"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR00080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HET6417999.1"
                     /translation="MQMEKQRARMVEQQLRRRGIGDTRVLAAMAEVPREAFVPEAHRA
                     NAYADGALPLAHGQTISQPLMVAMSVEALRLQGDETVLEIGAGSGYQAAVLSKLAKKV
                     YAIEIIPELVTNARRVLDSLGIDNVELICGDGRKGWPAGAPYDGIVVAAAAEKTPPAL
                     IEQLEEGGRLVIPVGGSWGQSLRVSRKRGDGLESEDLCRCVFVPLVYGG"
     gene            3253..3666
                     /locus_tag="VFG22_17025"
     CDS             3253..3666
                     /locus_tag="VFG22_17025"
                     /inference="COORDINATES: protein motif:HMM:NF015608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HET6418000.1"
                     /translation="MSTIVFRYLHFLGITFWIGTAVAVAIAASAPTQWESGIAQALRK
                     VTVRVTTPAMLFAFAGGFGMLIPSFAETYAKQGWVHAKLTLLLALAGATGVLTGKLRR
                     WADGQEVSSKTFGRLAWIISMLAILIITLAIFRPF"
     gene            complement(3676..4581)
                     /locus_tag="VFG22_17030"
     CDS             complement(3676..4581)
                     /locus_tag="VFG22_17030"
                     /inference="COORDINATES: protein motif:HMM:NF039685.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6206 family protein"
                     /protein_id="HET6418001.1"
                     /translation="MALVSKAEIEAFEGRLASAVRDGHAEDLDVIGYGEVTIAVKLTT
                     AHGSFACKRLVPFSSRAVAQQAASLIASYIEQLGRCGIDVVETETPIVECPEGNVLYC
                     VQPLLKPGSLGPDYLRGKSAEEAAPHVRRIFELIRASVTPSLAPDGQLSNWAFEGERL
                     RYLDVGTPFLRDSKGNDLFDFTEQTRALPAPVRVIVNRFLLRGILDNYHSTRGQALDF
                     LGNLVKEGLGEIFPPLVSVANEVFSLEPEITEDEVRAHYKSDAQTYAFIQAARRADRW
                     FFRKVLRKPYPYLLPPRIDRFGEGA"
     gene            complement(4581..5810)
                     /locus_tag="VFG22_17035"
     CDS             complement(4581..5810)
                     /locus_tag="VFG22_17035"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET6418002.1"
                     /translation="MSQEPHSEPFENFEDLASVFRQHVAPNKLAYYQRMGLQMVMGKR
                     EGIWFEDAYTKLRLINCHCNGGVFSLGHCNPRVTEAVAAALGKLDIGNHHFVSAQRTQ
                     LAARLSATLDDALPRVVFGVSGGEAVDVAIKAARGLTRRPGIVSAAGGYHGHTGLALA
                     AGDSQFRDPFGPNLPGFIQVPFNDVTAMEQAIDDQTAAVILEAIPATLGMPIPEPGYF
                     AEVRRLCSERGAKLLVDEVQTGLGRTGRWWGVQHEGVIPDAVISGKGLSGGIYPITAT
                     LLTREMHEVLDSEANPFVHISTFGGAELGCVAAQAVLDIVGEPGFLERVGALSERFAE
                     AFDPLPFVLRRRGLFMGLRFGSEMEALGALLRIMQQGVFCFPAGNDRQVLQFLPPLIL
                     TDDEAADLISRMTRAFA"
     gene            5922..7088
                     /locus_tag="VFG22_17040"
     CDS             5922..7088
                     /locus_tag="VFG22_17040"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF008398.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HET6418003.1"
                     /translation="MEDRPLNLAPASVADYRELARRRLPRQLFDYIDGGAYRETTMRS
                     NLSDLRDVRLRQRVLRDVSDIDLSADVLGERLSLPVVLAPVGLIGMFARRGEVQAARA
                     AEREGVRFCESTVSICSIEEVRQATKAPFWFQLYVMRDRAYAAALMERAQAAGCSTLV
                     LTVDLAVVGARYRDARNGMTESIPPRKKLVRAWDILSHPSWVWDVALGGKPLTFGNLE
                     AAVPGARSPNAFKDWVDSQFDPSVTWGDLDWVRQHWPGKLVLKGILDPEDAKEAARVG
                     ADAIVVSNHGGRQLDEVRSTASALPGIRDAVGDRLDVLVDGGVQSGLDVVKMIALGAK
                     ACLIGRAWAFAVAGGGEAGVAHVLRIIRDEMKVAMALTGNRRVEELGPDALDLL"
     gene            7273..8478
                     /locus_tag="VFG22_17045"
     CDS             7273..8478
                     /locus_tag="VFG22_17045"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6418004.1"
                     /translation="MNTALHAHGNAFPVAPEAASSTAWIDGLCADFSGHPFLRKSSDA
                     CPLIGRFAYALDRLAASAMSVPYKRKIHPHLSDTTLQEYVDYHAFYSQQRFLDAPGTA
                     FAAPPDGHSVIERATRRRSWDPTSASLASIDFYSPFQTLDPRFRNEYASLEPNDRVES
                     RAWFHRNGPRPVVIFLHGFSAPDYRINKMWFSAQKFYDAGLDVVFMNLPLHGGRMPKS
                     AAYHGTAIIQPSLWRLSEACAQGVLDLRILVDHLVKRGAPKVGIMGYSWGGFHASMMA
                     CLEPRTDFIVSVAPVVSIADLVMSWPTRSFFEDALDEPAMMIRELRKILAPFTPLSHS
                     LSIPKDRVLLVASGNDGIIPAVHTEALWKHFGRPEVYWSCGGHILYFDKGRVTERALR
                     FLQNIEVIA"
     gene            8483..9718
                     /locus_tag="VFG22_17050"
     CDS             8483..9718
                     /locus_tag="VFG22_17050"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET6418005.1"
                     /translation="MQIRGYSHPDFRAMREVVKWCVPSRGPGGAAISVYHRGELVADL
                     ACGTRDRQGRPFEPETLALSMSTGKGVMATLLHQMVDRGLVDLEAPVAKYWPEFGKNG
                     KGTITLRQAASHRAGLYSIARIIDSSEEMFDWNHMVHVIEDMWPVHKPDTDFGYHAWT
                     GGWIIGEVLQRVSGKSFPELLKENLADPLGLDGLYMGLPESELPRVAEFLIPSIPRRV
                     LQGRRRGVRAQVRRTASSTARWHSAVGQVVDAMIPPGLGDVLGDPRWLTSVIPSGNGV
                     FNAHSLARLYGALSRGGALNGTRLISASALAEATEDQNPRIGRVIPFPLRFKVGYMRP
                     VSLGLRLSLGKRHFDLGLPNPHAFGHFGFGGSGAWADPERELGVAIVTNCFGGRIPGD
                     LRPVAVATASVRCAEQRTS"
     gene            9762..10517
                     /locus_tag="VFG22_17055"
     CDS             9762..10517
                     /locus_tag="VFG22_17055"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET6418006.1"
                     /translation="MPTLALLFLLTAVLYASVGFGGGSTYNALLVLADTDYRILPSVA
                     LVCNLIVVAGGVWQYRRSGDLNLAFALPFVALSIPLAWFGGSVPIERETFVMLLGVSL
                     LAAGLVMWFQAPESKSASSGSWPVLWLAGLPLGAVIGFLSGMVGIGGGIFLAPALHLL
                     RLGDPKRIAATSSFFIMVNSIAGLVGQATKRGSAAHLAQLADYFWLGVAVLIGGQIGS
                     RLSAEALSGRVVKRLTAVLVLYVAGRLLYLSFS"
     gene            complement(10525..11307)
                     /locus_tag="VFG22_17060"
     CDS             complement(10525..11307)
                     /locus_tag="VFG22_17060"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HET6418007.1"
                     /translation="MAPGAGGFLDGLSRDPRQLRVGIATATDVFGVPMEPPSLEAVHQ
                     TTSLLSDLGHVVEEVPLPYDEWEVLRASIILGATNTAAVVTGLEARYGPAKVRGSLEE
                     VMLLLAAVGRSLPAEAVGSSRLLGCRVGLALSRFYERYDLLVTPTLGRVPQRLEDAEP
                     KPAEQRLLRFMVSRAAAGLFHLPQLRERVMEAQMQAFSQQVLHRTMVANLTGVPAMSV
                     PLHRTENGLPVGVQFIARFGDEATLLTLAAQLERARPWRVRR"
BASE COUNT         2069 a         3517 c         3534 g         2190 t
ORIGIN      
        1 gactggtttg gagagatgtc gatcatcgat gtgcagccgc gttccgcgag cgttcgggcg
       61 gtagcaccga cgttggttct ctccgtgacg gcagaacacg tcgagaacct gctctaccgg
      121 cgcgacgtca aggactacgc gctcttcatc atgaacatcg cgcgcgagct gagcaggcgc
      181 cttcgtgtgg cggatggaat tctggcccag ttctacgccg ccctatccca cagatacggc
      241 gcaccaccga agccttgagg caacgacagc gaagagccgc acttagaaca ccagaggtgg
      301 tggtctggtg agccccttga gcacttcccg gtctttgagc gcttcgagaa tgatcgtcgc
      361 ggtttcctcg atcgcacagt tcgtgacgtc gacgaccatc cactcggggt tttcgcgcag
      421 aatcgagtgt gcgtattcga gctcggcctt gacgtgatca cgtaggccgt agttcgcatc
      481 cgcgggcatt ccgagttgct ggagcctttg ctttcggatg tccctcagct ggtcgacgtc
      541 gatggttagc ccgacgaccc ggtaccccgg cagctcgtag acttccttgg gcagtgcgac
      601 gccgagaacg attggcacgt tcgcgacttt gagccctcgg cccgccaagt aagtggaaag
      661 cggggtcttg ctcgttcggc tcactccaac caaaacgagg tcggccttat ccaagttgcg
      721 tggttctttt ccgtcatcac ttttgacggc gaactcgagc gcttctacgc gacggaagta
      781 ctccggcgac agcggcaact cggccgtcgg gatgttcatc ggatcggcct cgaggtagtg
      841 gccgacgtga tggatgagcg acgcgattac gtcgacgtga cgcacctgga gccgagacgc
      901 cacctcgttg aaatggtcgc gcagctccgg gcgcacgagc gtgaatacga ccatcgcttc
      961 ctgggcggct gcactctcca gaatcgctgt cagggcccgt cggtcacgaa cccgtcgctc
     1021 gatcttgaga cggatgcgat ggttggggaa ttgaaggagt gtggcccgcg tgactcgctc
     1081 ggcggtctcg ccggtcgagt cgctgaccac atagatgacc ttcgcatcgg tggaagccaa
     1141 ggctcagccg cggcggccca gcttgcgccc gtgcatccgg ttcacgcgac ggcggttctt
     1201 cgccttgcgc tgcgccttgc ggcgggcggc cttgttgcgc tttcgcgagc ggtcgctgtt
     1261 gacgatgttc aagtacatga atgactccta agacgggggt ctaagggatg gccgggaggt
     1321 tcgcaagccg tcgaggcagg cccgagccac ctggattgaa atcacctctt tcggcgagga
     1381 atgaggagcc aatagtccaa atcgagcacc acgctcttct cgtatacctc cctgccgtcc
     1441 ttcgagcgct tgagcgggaa tcctgcttcg gcggggcgaa cgtctttgag cccaaccagc
     1501 ctcactcgga gggcaccgca gtccgaacga cccggcaggt cggctttgtc gagaatctcg
     1561 ctgtaggcgt agatcgtatc accggcgaac gtggggttcg cgtgcgctcc tccattccag
     1621 gcaaggatgc cgagcacctt ctccatccca ttgaaggaaa gagagcgcgc gatgctgatc
     1681 acgtggccgc cgtacatcag tcggcgtccg aagcgagtat ctttcgtctg cacggcatcg
     1741 aagtgaacct tggcggtgtt ctgatagagg cgcgtagcga tggcgtgctc tgactcctcg
     1801 atcgtcatgc cgtcgacgtg gtcgatcttc tcgccgggct cgtagtcctc gaagtacgcg
     1861 ttgccaccac tcggccaatc ctcataggca ctgaggtcga ggtccttggg aaccagcaac
     1921 tccgctggag tcacctcctt cggcattgcc ggccgatcgg ctgcaccggt cggcatggat
     1981 gggtctcgct tgttgaccat cacccagcgg aagtactcga gcacgtcttc tcctcgctgg
     2041 ttcgtaccga cggtcttgac ccagacgacg cctgtcttcc cgttgctgtt ctccttcttg
     2101 ccaattacct tggaaatgga tcgcagcgtg tcacccgcgt acacgggggt gagaaaccgg
     2161 acgcctgcat aaccgaggtt cgcaacggcg ttcagtgaga cgtcattgac ggtcttgcca
     2221 aagacgatgt gaaaaacgag catgtcgttg atcgtttcgc gcgggtatcc gagtgaccgt
     2281 gcgaattccg ccgagcagtg cagcggatag cgtgagccgg tgagcccgat gtagagcgcg
     2341 gcctcacctt cggtgatggt ccgcggcacc gcgtgcacga gctctgcacc gacttcgaaa
     2401 tcctcgaaaa aattgcccgg attggtcttg gccatgttct tcctcacttg cgacggccgg
     2461 actctagccc gtaaagcggg ctctgtcgcg cgggccgcgc ccgttggcgt cctctgcgat
     2521 tgccagatca gcaagcccgc gaaccgctcc gactcccact ggtgcaggag tccgatcgag
     2581 tggtatctag tgaaccgatg cagatggaga aacagcgcgc gaggatggtg gagcagcagc
     2641 tccgacgtcg cggaattggc gacacgcggg tgttggcggc catggccgaa gtgccgcgtg
     2701 aagcgttcgt gcccgaagcg cacagagcaa atgcttacgc ggacggcgct cttcctctcg
     2761 ctcacggaca gacaatctcg caacccttga tggtggcgat gtcggttgaa gcgcttcgac
     2821 tccaagggga cgaaaccgtc ttggaaattg gcgccggctc cgggtaccaa gccgcagttc
     2881 tgtccaagct cgcgaagaaa gtgtacgcga tcgagatcat tcccgagctc gtcacgaacg
     2941 cgcgccgcgt tctcgattca ctcggcatcg acaacgtcga gctcatctgt ggggacggcc
     3001 gcaaaggctg gcctgctggt gcgccatatg acggcatagt cgtcgctgcc gcggccgaaa
     3061 agaccccacc cgcgttgatc gagcagctcg aggaaggcgg tcgcctggtg atccccgtcg
     3121 ggggaagttg gggtcaatcg cttcgggttt cgcgcaagcg tggcgatggg ctcgaatccg
     3181 aagacctttg tcgatgcgtc tttgtgccgc tcgtgtatgg cggctagact ccgccccgtt
     3241 gaggagaacg caatgagcac gatagtcttt cggtaccttc actttctcgg gatcacgttc
     3301 tggataggaa cggcagtggc ggtggccatc gccgcgtccg cgccaacgca atgggagagt
     3361 ggaatcgctc aagcgctgcg caaagtcaca gtgcgcgtga cgacgccggc tatgctcttc
     3421 gcattcgcag gcggttttgg gatgttgatc ccgagcttcg ccgagactta cgcgaagcaa
     3481 ggttgggtac acgcgaagct gacactcctt ctggcactcg cgggcgcgac aggcgttctc
     3541 acggggaagc ttcgtcgatg ggctgatggc caagaagtgt cttcgaaaac cttcggtcgc
     3601 ctggcctgga tcatttccat gctcgcgatc ctcatcatca ctctggcgat cttccgcccc
     3661 ttctgaagcg cggccctacg cgccctcgcc gaagcggtcg atgcgcggcg gcagcaggta
     3721 tggatacggc ttgcgcaaaa ccttgcgaaa gaaccagcga tcggcgcggc gcgcggcctg
     3781 tatgaaggcg taggtttgcg cgtcactctt gtagtgagcg cgcacttcat cctcggtgat
     3841 ctcgggttcg agggaaaaca cttcattcgc aacggaaacc aaaggcggaa agatctctcc
     3901 aaggccttcc ttgacgaggt tgccgaggaa atcgagcgcc tggccgcgtg tcgagtgata
     3961 gttgtcgagg atgccgcgca agaggaagcg gttgacgatc acgcggaccg gagccggaag
     4021 cgcgcgcgtc tgctcggtga aatcgaatag gtcgttgccc ttcgaatcgc ggaggaacgg
     4081 cgttccgacg tccagatagc gcaaccgctc gccttcgaag gcccagttcg acagctgtcc
     4141 gtcgggggcc agggacggag tgacggatgc acggatgagc tcgaaaatgc gtcgaacgtg
     4201 cggtgccgcc tcctccgcgg atttgccacg cagatagtcc gggccgagcg agccgggctt
     4261 caacaagggt tggacacaat agagcacgtt gccctcgggg cattcgacga tcggagtttc
     4321 ggtctcgacc acatcgatcc cacagcgccc gagctgttcg atgtacgatg cgatcagact
     4381 cgcggcctgc tgggccaccg ctcgcgaaga gaacgggacg agtcgcttgc aagcgaagct
     4441 cccgtgcgcc gtcgtcaact tgaccgcgat cgtcacttca ccgtagccga tcacgtcgag
     4501 gtcctccgcg tgcccgtcgc gcacggctga ggcgagtcgc ccctcgaatg cttcgatctc
     4561 cgctttgctt accaaggcca tcaagcaaag gccctggtca ttcgcgaaat cagatcagcg
     4621 gcctcgtcgt cggtgaggat caagggcggc aggaattgca gcacttgccg atcgttcccc
     4681 gcggggaaac agaatacgcc ctgctgcatg atgcgcagaa gagcaccgag ggcctccatt
     4741 tcgctaccaa acctgagccc catgaagagg ccccgacgcc tcagaacgaa ggggagcgga
     4801 tcgaaagcct cggcgaatcg ctccgacagc gcaccgacgc gttcgaggaa ccccggctcg
     4861 cccacgatgt cgagaaccgc ctgcgcggcc acgcatccga gctcggcgcc gccgaacgtc
     4921 gagatgtgga cgaacggatt cgcctccgaa tcgagaacct cgtgcatttc gcgcgtcaga
     4981 agcgttgctg tgatcgggta gattcccccg gacagtccct tgccgctgat gaccgcatcc
     5041 gggatcacgc cttcgtgctg cacgccccac cagcggcccg tgcgcccgag ccccgtctgc
     5101 acttcgtcga cgaggagctt cgcaccgcgc tcgctgcaaa gccttcggac ctcggcgaag
     5161 tagccaggtt ctggaatcgg catgcccaaa gtcgcaggaa tggcttctaa aatgaccgcg
     5221 gccgtctgat cgtcgatcgc ctgctccatc gccgtgacgt cgttgaacgg cacctgaatg
     5281 aacccgggaa ggttcggacc gaagggatcg cggaactgtg agtcgcccgc cgccaaggcc
     5341 aacccggtgt gcccatgata tccacccgcg gccgatacga ttcctgggcg acgcgtcaag
     5401 ccccgtgcag ccttgatggc gacatcgacg gcctctccac ccgatactcc gaacacgact
     5461 cgaggaagag cgtcgtcgag cgtcgcggag agccgcgccg ccagctgtgt gcgctgagcc
     5521 gatacgaaat ggtggttgcc gatatcgagc ttgccgagcg ccgcagctac ggcctcggtc
     5581 acacgcggat tgcagtgacc gaggctgaag accccgccgt tgcagtggca gttgatcagg
     5641 cgaagcttgg tatacgcgtc ctcgaaccag atgccttcgc gttttcccat gaccatttga
     5701 aggcccatgc gctggtagta cgcgagcttg ttgggtgcga cgtgctgacg gaagaccgaa
     5761 gccaagtcct cgaaattttc gaatggctcc gaatgtggtt cctgcgacat ccgctgcagt
     5821 ctacctcagc tctctgcttt gttcagcatc cttcgggcgc agggtcgaga cgcgccgagg
     5881 gctcttgacg aagaggagga cgcgggagat cgtgccgacc catggaagac cgaccgctca
     5941 atctcgctcc cgcaagcgta gcggactacc gagagctagc gcgcagaaga ctgccccgac
     6001 agctctttga ttacatcgat ggtggcgctt atcgggagac gacgatgcgg tccaacctgt
     6061 cggacctgcg cgacgttcgt ctgcgacagc gagtgcttcg cgacgtttcc gacatcgacc
     6121 tcagcgcaga cgtgctcggc gagcggctct cactccccgt ggtcctcgcg ccggtgggcc
     6181 taatcggaat gttcgcgcga cggggtgagg tgcaggcagc gagagccgcg gagcgagagg
     6241 gcgttcggtt ttgcgagtcg acagtctcca tctgctccat cgaagaggtg aggcaagcga
     6301 cgaaagcgcc attctggttt cagctctacg tgatgcgaga ccgcgcctac gcggcagcgc
     6361 tcatggaacg cgcgcaagcg gccggctgct cgactctggt tttgaccgtc gacttggccg
     6421 tcgtcggtgc tcgctaccgg gacgcacgaa acggaatgac cgagtccatc ccgccgcgaa
     6481 agaagctcgt tcgcgcctgg gacatcctct cgcacccgag ttgggtttgg gacgtggcgc
     6541 tgggcggaaa accgcttacg ttcggcaacc tcgaagcagc agtgccggga gcgcgttcac
     6601 ccaatgcgtt caaagactgg gtcgacagtc agttcgaccc gagcgtcacc tggggggacc
     6661 tcgattgggt gcgtcagcat tggccgggca agctggtgct aaagggcatt ctcgaccccg
     6721 aggacgccaa ggaggctgcg cgagttggcg ccgatgcaat cgtggtttcg aaccacggag
     6781 gccggcaact cgacgaggtc cgatccaccg catccgcgtt gcctgggatt cgagatgccg
     6841 tgggagaccg gctcgacgta ctggtcgacg gcggagtgca gagcggactc gacgtcgtga
     6901 agatgatcgc gctcggagcc aaggcatgcc tcattgggcg cgcatgggcc tttgcggttg
     6961 ccggaggtgg tgaagccgga gttgcacacg tgcttcgcat cattcgcgac gagatgaagg
     7021 tcgcgatggc gctcacgggc aaccgccgag tcgaggagct tggacccgac gcgctcgatc
     7081 tgctttgagg ctgatgccgg tttcgagctc catcgcgcaa cggctgcgcg gacaaattga
     7141 tgtgacgcag tttctgcgct gaactttgac gggaaattgc tgaattgcct gacaaagcgc
     7201 gaagctattg ggcaacggca cccgaattgc agatggccca agcgttccgc tccgagcgcg
     7261 aagagaggtt tcgtgaatac agcactgcat gcccatggca acgcgttccc agttgcgcct
     7321 gaagcggcgt cgagcacggc ctggatcgac ggcctctgcg ccgatttttc agggcaccct
     7381 ttcctgcgaa agagcagtga cgcctgcccc ttgatcggca ggttcgccta cgcgctggat
     7441 cgtctcgcag cgtccgcgat gtcggtaccc tacaagcgca agatccatcc gcatctctca
     7501 gacacgacgc tgcaggagta tgtcgactat cacgcgttct actcgcagca acgtttcctc
     7561 gacgctcccg gcacggcgtt cgctgcgcca cccgacgggc actcggtcat cgagcgcgca
     7621 acccggcgtc gttcctggga ccccacgagc gcatcactgg cgagcatcga cttctacagc
     7681 ccatttcaaa cgctcgatcc acgcttcaga aacgaatacg cgtcactcga gccgaatgac
     7741 cgtgtcgagt ctcgcgcctg gtttcatcgg aacgggccgc gccccgtcgt catttttctc
     7801 catggcttct cggcgcccga ttaccgcatc aacaagatgt ggttctccgc gcagaagttc
     7861 tacgacgcag gcctcgatgt ggttttcatg aacctcccgc ttcacggcgg gcgcatgccc
     7921 aagtcagcgg cctaccacgg caccgcgatc atacagccat cgctctggcg gctgagcgaa
     7981 gcctgcgccc agggcgtgct ggatcttcgg atcctggtcg atcatctcgt gaaacgtggg
     8041 gccccaaagg ttggaatcat ggggtatagc tggggtggct tccacgcttc catgatggcg
     8101 tgcctggaac ctcggaccga tttcatcgtg agcgtcgcac cggtcgtgag cattgcagac
     8161 ctggtcatga gctggccgac gcgctcgttc ttcgaggacg cgctcgatga gccggcgatg
     8221 atgatccgcg agctccgaaa gatcttggcg ccgttcaccc cactctcaca cagcctcagc
     8281 attcccaagg acagagtcct tctcgtcgca agcggcaacg acggcatcat tccggcggta
     8341 cacaccgaag cgctctggaa gcactttggc cggccggagg tatattggag ctgcggcggc
     8401 cacattcttt atttcgacaa aggacgtgtg accgagcgcg cattgcggtt tctccaaaac
     8461 atcgaggtca tcgcttagga acatgcaaat tcgtgggtac agtcatcccg acttccgtgc
     8521 gatgcgcgag gtggtcaaat ggtgcgtccc gtcgcgtgga ccgggcggcg ctgcaatttc
     8581 cgtgtatcac cgcggcgagc tggttgccga tctcgcttgc ggcacccgag atcggcaggg
     8641 gcgcccgttc gagccggaga ccctcgccct cagcatgtcg actggcaagg gcgtcatggc
     8701 caccttgctc caccagatgg tcgaccgtgg gctcgtcgac ctcgaggcgc cggtggcgaa
     8761 gtactggccg gagttcggga agaacggcaa gggcacgatc accctccggc aagcggcgtc
     8821 acaccgtgcg ggcctctact cgatcgcgcg aatcatcgat tcgtccgagg agatgttcga
     8881 ctggaaccac atggtgcacg tgattgaaga catgtggccc gtacacaagc cggacaccga
     8941 cttcgggtac cacgcatgga ctggtggctg gatcatcgga gaagtccttc aaagagtgag
     9001 cggcaagagc tttccagagc ttctgaaaga gaacctcgcc gatccgctcg ggctcgatgg
     9061 gctctacatg ggcttgccag aaagcgagct gccacgcgta gccgagtttc taatcccgag
     9121 catcccacgc agagtcctac aaggacgtcg ccgcggcgtg cgcgcgcagg tgcgccgtac
     9181 cgcgtcgagt accgcgcgct ggcattcggc agtgggtcag gtcgtcgacg cgatgattcc
     9241 gccaggcctc ggcgatgtgc tcggcgatcc gcgatggctc acttcggtaa tcccgtccgg
     9301 caacggagtg ttcaacgcac actcccttgc gcgattgtac ggcgcgctca gtcggggcgg
     9361 cgcactcaac ggcacacgct tgatctcggc ctcggcgctt gcagaagcca ccgaggatca
     9421 aaatcctcga atcggacgcg tgatcccgtt tcctctgcga ttcaaggtcg ggtacatgcg
     9481 gccagtctcg ctcggtctgc gcctatcgct cgggaaacgg cacttcgacc tcggactgcc
     9541 gaacccacat gcctttgggc acttcggttt cggaggctct ggcgcctggg cggatcccga
     9601 acgggagctc ggtgttgcca tcgtgaccaa ctgcttcggt gggcgcatcc ctggcgacct
     9661 tcgccccgtt gccgtagcca cggcgagcgt ccgctgcgcc gaacagcgca cgagctagca
     9721 tcgcggttgc gcaggcgagt agcctcccgt atcgagtgcg gatgccgacg ctcgcgctcc
     9781 tgtttctgct gactgccgtg ctttacgcct cggtcggatt tggaggcggt tccacttaca
     9841 acgcgctctt ggtcttggcg gataccgact accgcatcct tccgtcggtt gccctggttt
     9901 gcaatctcat cgtagtcgcc ggaggcgttt ggcagtaccg ccgctcgggg gacttgaacc
     9961 tcgcgttcgc tctgccgttc gtcgcgctct cgattccgct cgcctggttc gggggcagcg
    10021 tccccatcga acgcgaaacg ttcgtcatgc tcctcggcgt ctcgctgctc gcggcaggcc
    10081 tcgtgatgtg gtttcaagcg cccgaatcga aatctgcatc atcaggttcc tggccggtcc
    10141 tttggctcgc aggtttaccc ttgggagccg tgatcggttt cctttccggg atggtcggga
    10201 tcggcggagg tattttcctg gcgcctgcct tgcacctgct tcggctcggc gaccccaaac
    10261 gcatcgctgc gacgtctagc ttcttcatca tggtgaactc gatcgccggc ctggtcggcc
    10321 aagctacgaa acggggcagc gcggcgcacc tcgctcagct tgcggactac ttctggctcg
    10381 gcgtcgcggt tctgatcggc ggtcagatcg gcagccgcct cagcgccgaa gcgctctccg
    10441 gacgggtggt caagcggctc accgccgtgc tcgtcctcta cgtcgccggt cgattgctct
    10501 acctcagctt ctcctgagcg acgttcagcg gcgcacgcgc cagggtcgcg cccgttcgag
    10561 ctgggcagcc agggtcagga gcgtcgcttc atcgccgaag cgagcgatga attggacgcc
    10621 taccggtaga ccgttctcgg ttcggtgcag gggaaccgac atcgcgggaa cgccggtcag
    10681 attcgcgacc atcgtgcgat ggagcacctg ctgcgaaaaa gcctgcattt gcgcctccat
    10741 gacccgttcc cgaagttgag gcagatgaaa cagcccggcg gcggcacggg agaccatgaa
    10801 acggaggagc cgttgctcgg ctggcttcgg ctccgcatct tcgaggcgct gaggcacccg
    10861 tccgagggtg ggtgtcacga gcagatcata cctctcgtag aacctcgaga gggccagtcc
    10921 aacacggcac ccgaggagcc gcgagctccc cacggcctcg gccggaagcg atcttccaac
    10981 ggctgcgagc aggagcatga cctcctcgag cgagccgcgg accttggcgg ggccgtacct
    11041 cgcttccagg ccagtcacca cggcggccgt attggtggcg ccgagaatga tcgaggcccg
    11101 gagcacctcc cattcgtcgt agggcagcgg aacctcctcc acgacatggc ctaagtcgct
    11161 caaaagacta gtagtttggt gcacggcctc gagcgacggg ggctccatcg gcacaccgaa
    11221 aacatcggtg gctgtcgcta taccgacacg caattggcga ggatcgcggc tcagtccatc
    11281 cagaaaacct cccgcacccg gtgccacata
//
LOCUS       DASRZF010000384        16997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_289007, whole genome shotgun sequence.
ACCESSION   DASRZF010000384 DASRZF010000000
VERSION     DASRZF010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 16997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16997
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289007"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /locus_tag="VFG22_17065"
     CDS             <1..977
                     /locus_tag="VFG22_17065"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET6418008.1"
                     /translation="PPEDISDLSPAEIEPFKTEFDVREGLRFLGHEVSMVGLSDELGP
                     LRTSIEEIKPHVVFNLLEEFRGQAIFDAHVVGYLELQGIPYTGCNPRGLLLARDKALS
                     KKVLAYHRIRVPRFHVFPRYKKIKRPKQLGFPLIVKSLIEEGSYGIAQASVVHNDKTF
                     EERVAFIHDKIRTDAVAEQYIPGREVYVSVLGNHRLQVLPTWEIFLDKLPEDAAKIAT
                     RKVKWDLEYQEKHGVRIGRAKGISDEMERRIARLSRRICRGLGTDGCVRIDFRLDEAG
                     ELYFLEANPNPDIGEGEEFAGAAEAAGMNYPSLLQKIVNLGIRRAERG"
     gene            complement(987..1475)
                     /locus_tag="VFG22_17070"
     CDS             complement(987..1475)
                     /locus_tag="VFG22_17070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418009.1"
                     /translation="MSYTRMNWMRFMALLTCGFALMACGESSTAEPRLRELVFCENPD
                     VSAPFCALSGYSMADDSALRTKLEGCASAGCHGTGGTAVTTWNLDLSASSVQGALIPL
                     TSVIGLSGDYLVDEFDPDCSYMLTKVTERPAGGSRMPLTPPHWSSDEIDCFRAYLNEM
                     SN"
     gene            1717..3894
                     /locus_tag="VFG22_17075"
     CDS             1717..3894
                     /locus_tag="VFG22_17075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418010.1"
                     /translation="MQKSHWCINGNLQAGFVLLLLGSALALPGCKSEPTLFGGCSTQN
                     LTACPQSEGFANPYEGQLELLTWPETLRKASLELTGKLPTERQTQMTIEHGQQGLEAA
                     LAEMMRDDGFYDRMIELYNDHFLTDKYLGRENAIELLDEEVWPNLRWFEKKYPDDEAK
                     ARVTNDSLAREPLELIAHVIRTERPFTEILTANYTMANGYSQLSMVGLPDVPLGIGEN
                     PESFREVKIPGIPHAGILTSAMFLNRFPTSDTNINRHRSRMTYWFFLDIDINQFGSRP
                     VDASNDFGENPTLNNPDCTVCHTTMDPVAGLYMNWNESGEYSGKGEWFDADYILPPGF
                     NGEALPAESRRNALQWFAERVALNPRFAQATVKTVFQGLTGQKILDISVPMTPEMASD
                     AMQEPDPMVDPDPMVDPAAGMDAGVGGMGGMGGIGGAAGLGGMGGTTPPPPPPPPLPE
                     VDPEIDPDVNPALERAYNVQQQILRTIQRRFIESGHNFKLLVLEIVLSPYFRAKNASP
                     LTSEMEIELSTFGTARLLTPEHLSRKIEATTGVRWRARPDRTDYLLDQYRIFYGGIDS
                     DLVTQRVTEPNGVMASLAQRMAYEVACLAVPYDFSKPANQRVLFPNIDRNAPLSEASD
                     QVRDTVRYLHERILGESLAPTDEEVEATMVVLMEAYSLGRQGLADGSVPEDLAGPCRL
                     NEDRITGESLPDERRINRDTEYTVRAWMAAVSYLLSDYRYLYE"
     gene            3908..5194
                     /locus_tag="VFG22_17080"
     CDS             3908..5194
                     /locus_tag="VFG22_17080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HET6418011.1"
                     /translation="MERRDFLRIASWAGLTCVLPAVMDPLRSSAQSSGYTGTYWIFMH
                     AGGGWDPTHLCDPKGQATANENNPMGRFLKSDIRTAGNISYAPLGDDGTALDAFFQTH
                     YQRLLVINGMDTTTNGHDQGTRGTWSGRTAEGHPPIGALIAATHGPNLPMPLIGYGGY
                     LDTQGVTNAVPLGDGGLLHNLAHPSRPDPTGSDENYFGHPDVPSIVQREQQLRLLRLQ
                     GKQRLPRIWDGQNELMLARLGQDDLKRVSDSLPENFSNNQLERQIQVALAAYQAGLSV
                     SVSVSMGGFDTHGNHDNNHLPRLIDFVNGANFAMQEAERMGIGDKVAVVMGSDFGRTP
                     GYNNGNGKDHWPISSMMMMGPGIPGNRVIGGTDDRHGLQLIDPVTLQATGSPDSAQGT
                     RLSIAHVHRALRNLSGVPDQYKAAFPLAGEDLPIFD"
     gene            complement(5191..5562)
                     /locus_tag="VFG22_17085"
     CDS             complement(5191..5562)
                     /locus_tag="VFG22_17085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418012.1"
                     /translation="MFVLLALAVALFIFVMGAWGVFAPGSIFAFISGWSSKTGFWLAV
                     VLRLCFGLALWFAAPDTRSPVLLQALGALAILLGLGLPIIGYERFERVLDWWIGHSPF
                     VMRLWSLLATAIGGLVLWSLT"
     gene            complement(5671..6570)
                     /locus_tag="VFG22_17090"
     CDS             complement(5671..6570)
                     /locus_tag="VFG22_17090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418013.1"
                     /translation="MSFLRGWGAIGLLLAGMMWLAPGEARAYPDEPFSLDEGEKAHTD
                     RFHILGEIGVYAGKGEADQKQVVISPLIEMRLQLAYSFLMQVVWGMSYANLNLEDGGS
                     DNSFRFGNPYVAFHYQGKKNQFSYRVGVGATIPAATLPDDINEQVTAATAYKDAAAMR
                     GNRMFWLWDPHTVSVIVPIAFERRKPSGFLWGAYLDTGALIKLNDKNPRTSKTDFIMQ
                     MAAMMGYQATDWLRVGSDFSLVLIPKYQEQKTQLAVQPFMRFGNENAFGALLLNINID
                     NPNGFSFDSRRVWGLRIAGGASF"
     gene            complement(6693..7862)
                     /locus_tag="VFG22_17095"
     CDS             complement(6693..7862)
                     /locus_tag="VFG22_17095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418014.1"
                     /translation="MLHRASRTILVLLGFSLVLLGCDSSSESAAGTGGSAGTGGIGGA
                     GGAPRLVVTADWLNQSLTLFDYDKLVDGQSDASQAIVDTIDLSNWEPGPIELEITPDG
                     KTAVVSVGPAFFDSLSGLLGDPEIPPGGTLLIVDLETGTADEIQTEDVPLGIAISPDG
                     RRAYTANYGTADARGDTLSVIDIPGRQLIEDVPVGSGPEQVALSPDGSLGIVNVVSGG
                     GIRIFQTSDVAGTLSEVLETGSDPSGVSFLDGNDRAVVANSFGFDVTLVDTSNPSSPS
                     VIESVPVGVGVPYGVTYCAVRNQILAPTNATGRDLPTQLVTFTVSAESLSAGPPLPLS
                     GGNFPLTAAIDSACDFAFVAHIVDRKLSIIDLASNAVRAIDWLAEPGPSYVAVQP"
     gene            complement(7893..8828)
                     /locus_tag="VFG22_17100"
     CDS             complement(7893..8828)
                     /locus_tag="VFG22_17100"
                     /inference="COORDINATES: protein motif:HMM:NF014646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET6418015.1"
                     /translation="MTNNVKEISGFYPMRVAARLTGLTADTIRVWERRYGVVDPDRTE
                     GNKRRYTGNDVRRLVLLRRATELGHSIGQIASLPDEEVRRLIGETGPDVSSTVSLYAA
                     VVEDYLEAIFDYDVQRAESMLTRTAAILSPMTLTLEVLVPLMRRVGEAWNSDQLRIAH
                     EHIISGQLRSLLGTLMRHAEPVTGAPRVIVATPPRHLHEFGAIIGAFMAASRGFEPIY
                     LGTHMPFSEISEAAKQSGAKLILLSIARDCEPDESQQLVAGIELLARSHQVWLGLPAT
                     HELASYRLPATLLHRYEDLDAALTADPAGAQQNLG"
     gene            complement(9000..9530)
                     /locus_tag="VFG22_17105"
     CDS             complement(9000..9530)
                     /locus_tag="VFG22_17105"
                     /inference="COORDINATES: protein motif:HMM:NF014522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HET6418016.1"
                     /translation="MQTKSILISALASIALLGCAKDAPSTAASTAASTAARINQPNIV
                     AVAAGSGQFSTLVAALTAADLVETLQSPGPFTVFAPTDEAFAKLPAGTVDSLLLPENK
                     AKLTAILKYHVVAGEVLAADVVKLSSADTVEGQPVTITAGNAGVRINDANVIKTDVMA
                     SNGVIHVIDTVLIPSS"
     gene            complement(9534..10973)
                     /locus_tag="VFG22_17110"
     CDS             complement(9534..10973)
                     /locus_tag="VFG22_17110"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET6418017.1"
                     /translation="MVSGPRPKVVIAGASGFVGRSLAPALSAAFEVIGLSRTEREPGG
                     GFDEFRVCDLFSLKDAEDALRGAEYAVYLVHNMMPSARLTQGDFADLDLVCADNFARA
                     AAGAGVKQIVFLGGLTPESGRLSSHLQSRCEVEKALGEHGVPVTTLRAGLILGGQGSS
                     FQLMARLVERLPVMVCPRWTNIETQPVALADVIQFISGVVGRQEHFGRTYDIGAPDVV
                     TYKQMMAMIASELGKRRLFIPSRFMSPGLSRLWVTLITGAPKSLVAPLVQSLKHKMVV
                     RDHGLAEELGISRTSTRDAIRQALAESDGTVPHAFRGFVSRERLVRSVQRMRLPKGWT
                     ALRAAAEYLDWLPRFLNGLVRVELDSPERFSFVLWPFRASLLQLSRASDRSGSDRQLL
                     YVTGGLLSREGGKPRFELRQVLGGNTLLTVVHDYEPRLPWLVYVSTQAVFHRWLMGRF
                     AAHLGRYAAVGEPAVDSQKVLPSQTLLED"
     gene            11145..12119
                     /locus_tag="VFG22_17115"
     CDS             11145..12119
                     /locus_tag="VFG22_17115"
                     /inference="COORDINATES: protein motif:HMM:NF039286.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET6418018.1"
                     /translation="MTNEKKTNPWAGFARGWFLLMFSDELAPGQVVPLKRFDQELVLF
                     RTESGEAKVLDAYCPHMGAHLGHGGKIEGEGIVCPFHAWKFDGSGKCIEIPYAKRIPP
                     KAGLACWPVLERNGMIFVWNDQAKSAPDWEIPVLEEWDDPEFLPWRYAKLQIKTPPLA
                     IAENLADAGHFIPVHNTHPIPGSFSNTYDAHVGQQKIKAIAYPVHGGKDEFESTATYY
                     GPAYQVTKMKGKGLGVIINAHTPIDDEHVMLRFGVSMKATKGMTDELIEHFINNMRDG
                     YLQDVQIWENKIYLDRPILCDGDGPIGEFRRWYAQFYTPRNEAADAAE"
     gene            12134..13315
                     /locus_tag="VFG22_17120"
     CDS             12134..13315
                     /locus_tag="VFG22_17120"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-dependent monooxygenase"
                     /protein_id="HET6418019.1"
                     /translation="MSMLTKPDFVKLARDAKSRLRERNSRADAERVIPKETIEELKEA
                     GFFRMMQPKRWGGYECHPLEFFDAAIEIASACPSTAWVLGVVAVHNWQLALFPEQAQK
                     DVWEGQNGLDTLIASSYMPVGKVTPDDGGYRLSGKWGFSSGSDHCQWAFLGAFAPKEE
                     GRPPDMFTLLLPRSDYELIDDWFVSGLKGTGSKSVKVDDVFVPAHRVHRMSDAYRLQS
                     PGNDVNTSPLYRVPFGQLFTRTVATPAIGMLQGALEAYLALNRGRKGRADGKSASDDP
                     FNLETCARATLAIREVRALLRNDWNEMMSKAEAGEKIEVEDRVAYRANAGAATDRMVK
                     ALDELFMASGGSAIFLDQDIQRFFQDIHGARAHHANNPLKTQRNYGGLLMGEQTTDFF
                     L"
     gene            13353..14396
                     /locus_tag="VFG22_17125"
     CDS             13353..14396
                     /locus_tag="VFG22_17125"
                     /inference="COORDINATES: protein motif:HMM:NF039286.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET6418020.1"
                     /translation="MSKKPRFPIPRYPRGWFQVAYSDEIAAGEVKALKYFGVDLVMFR
                     TEDGEVNVLDAYCPHMGAHLGHGGKVVGDGIVCPFHAWKFNGAGKCIEVPYAEHLPRK
                     ANITAWPVHEVNGLVMVWHDIEGKAPAWEIPTIPEWNNEDWTPYWRQHWKIRTHNQDM
                     CENVVDKAHFRYVHGMNIVPQPHTINLEYPHIKMITDTIYETPQGEVKGELEVDVHGF
                     GFSLSRFRGIVETTNMASVTPIDEEHCDVRFSFTVKKIGGADITAGVGRAFTKEIARQ
                     LEEDTPIWEHKAFFERPMLCDGDGPVAEFRKWCKHFYPDWYHKQAQDEYDGVKEVSKP
                     LTLAERRRRLAAA"
     gene            14397..14828
                     /locus_tag="VFG22_17130"
     CDS             14397..14828
                     /locus_tag="VFG22_17130"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HET6418021.1"
                     /translation="MDRGSERRSPATPDEIRARIRSEHAAISAQLARVEALTERIGDD
                     DRESVIALRDELQVLGEILLEHLHYEEYQLPSLAGTSEVAQMVEDMGEEHRAQREIFD
                     SVIRELDETAVGSKLVVGVRELSRAIRDDMEHEERELLNKP"
     gene            14844..15038
                     /locus_tag="VFG22_17135"
     CDS             14844..15038
                     /locus_tag="VFG22_17135"
                     /inference="COORDINATES: protein motif:HMM:NF018595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HET6418022.1"
                     /translation="MKLMYNPATCNHYGVCVEEAPEVFSFAADGSLSIQSYDVPPDLE
                     VKVRDACMMCPTQSIQIEEE"
     gene            complement(15087..15356)
                     /locus_tag="VFG22_17140"
     CDS             complement(15087..15356)
                     /locus_tag="VFG22_17140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418023.1"
                     /translation="MAVIIEVIIPLAILGLVGWGLWLWYRLRLARAEQAERMQEELSE
                     LRAYKQDTEARLQALETIATLGEGRFRIDYKELKEDEDPTRKRKG"
     gene            15425..16948
                     /locus_tag="VFG22_17145"
     CDS             15425..16948
                     /locus_tag="VFG22_17145"
                     /inference="COORDINATES: protein motif:HMM:NF037725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ligase PGM1-related protein"
                     /protein_id="HET6418024.1"
                     /translation="MSGEVDEMNAFRELQSQLGPRWALADGDGSEPRDIVVVPSLSFD
                     GIDTVSIDGVLHYEERMLFALNLLRHPRARLVYLSSAPIHPAAVDYMLSLISGIPNEH
                     MRRRLTVLSLYDNSLVPLSQKILQRPRVIERLRQLIRPECGLMTCFTVSELERDLAVA
                     LGVPLYGLDPDLAWLGTKSGSRWAFRKAGIPLLPGVEDLRTEEDLVQAINSLRDENEG
                     LRRVVVKQNYGFGGQGNAILELGALDSASRNEDLKAALPNMRFVGQRQTWTSFMEKFE
                     REGGIVEAFLSDESKRAPSCQLRISPEGSLDALSTHDQLVDGIDGQIYAGCRFPSDAA
                     YRADIQKDALQVAAVLQERGAVGRLAVDFLCVRADDGTWRRYAIETNLRLGGTTHPMM
                     LLRMMTDGRYDADTGLYLTPRGEPQFYVATDSMQSDSYRGLLVEDLLDIAAVNGLHYQ
                     AWSESGVLFHFTGALSEFGKLGITAVGKSAAEADHWFERTRLVLDRETKSHPCTSKK"
BASE COUNT         3569 a         5152 c         5170 g         3106 t
ORIGIN      
        1 tgcctcctga agacatcagc gacctctcgc ccgcggagat cgagcctttc aagaccgaat
       61 tcgacgtgcg agaagggctt cgctttctgg gacacgaggt ctccatggtg ggactcagcg
      121 acgagcttgg gccgctgagg acctctatcg aggagatcaa accacacgtc gtgttcaacc
      181 tgctcgaaga gttcaggggg caggcgatct tcgatgcaca cgtggtcggt tatctcgagc
      241 ttcagggcat accgtacacg ggatgcaacc cacgaggtct gcttctcgcc cgtgacaaag
      301 cgctttcgaa gaaggtcctc gcgtaccacc ggattcgagt gccgcggttt cacgtcttcc
      361 ctcgctacaa gaagatcaaa cgcccgaagc aactcggttt cccgctcatc gtcaaatcgc
      421 tcatcgaaga gggtagctac gggatcgccc aggcatcggt ggtgcacaac gacaagacat
      481 tcgaagaacg agtcgcattc atccacgaca agatccgcac cgatgccgtc gcagaacagt
      541 acatcccggg ccgcgaggtc tacgtctcgg tacttggaaa ccaccggctt caggtgcttc
      601 cgacctggga gatcttcctc gacaagcttc ccgaggatgc ggcgaagatc gcaacgcgga
      661 aagtgaagtg ggacctggaa taccaagaga agcacggcgt caggatcggc cgtgccaaag
      721 ggatttccga cgagatggag cgtcgcattg cgagactgtc acgccggatt tgccgcggcc
      781 tcggaaccga cggctgcgtg cgcatcgact tccgactgga cgaggccggt gagctctact
      841 tcctagaagc gaatccgaat cctgacatcg gtgagggtga ggagttcgcc ggtgccgccg
      901 aggcagccgg catgaactac ccgtccttgc ttcagaagat cgtcaacctc ggcatccggc
      961 gcgccgagcg aggttgagcg gcgccctcag ttgctcatct cgttaagata ggcgcggaag
     1021 cagtcgatct catcactgct ccagtgcgga ggcgtgaggg gcatccggct tccccctgcg
     1081 ggacgctcgg tgaccttggt gagcatatag ctgcagtccg gatcgaactc gtcgaccagg
     1141 tagtcgccgc tcagaccgat cacgctcgtc aaaggaatga gcgcgccctg cacggaagag
     1201 gcggagagat ccagattcca agtggtgacc gcagtccccc cggttccgtg acagcctgcg
     1261 gaggcgcagc cttcgagctt cgttcgaagg gccgagtcgt ccgccatcga gtagcccgac
     1321 agagcacaga acggtgccga tacatcgggg ttctcgcaga acacgagctc gcggagccgg
     1381 ggctccgccg tgctgctctc gccacaagcc atgagggcaa acccacaggt caaaagggcc
     1441 atgaatcgca tccagttcat ccgtgtgtaa gacattttct aggtcgctcc tggtaaaaaa
     1501 atccggtcag ggtctaatca tgagcaaaaa aataagccgc accaagggaa aaattcagtg
     1561 cgactttccc ttttgatctt cgcagaatag cgagatgctg caagcctgat cacgaataaa
     1621 atagacccat aaggggcata tttcaactgt atgggcttgt attacacctc aaaattagtc
     1681 ctgccgggtg ttttttcgca tgttaggctg acacttgtgc agaagagtca ctggtgcatc
     1741 aacgggaacc ttcaggctgg attcgtcctc ctgctgcttg gctcggcact cgcgctgccc
     1801 ggctgcaaga gcgaacccac gcttttcgga ggttgctcaa ctcaaaatct caccgcctgc
     1861 ccccagtcgg aaggcttcgc gaacccttac gagggccagc tggagctgct gacttggccg
     1921 gagacgctcc gaaaggcgag cctcgagctc accggcaagc ttccgaccga gcggcagacg
     1981 cagatgacca tcgagcatgg tcagcaaggg ctcgaagccg cgctcgcgga aatgatgcgc
     2041 gacgacggct tctatgaccg gatgatcgag ctctacaacg atcatttcct gacggacaag
     2101 tacctcggcc gcgaaaacgc gatcgagctc ctcgacgaag aggtctggcc caatcttcgc
     2161 tggttcgaaa agaagtaccc cgacgatgaa gcgaaggccc gggtcacgaa cgactccttg
     2221 gcgcgtgagc ctctcgagct gatcgctcac gtcatccgca cggagcggcc tttcacggag
     2281 atcctcaccg ccaactatac gatggcgaat ggttactcgc agctgtcgat ggtgggtctg
     2341 cctgacgttc cgctcggaat cggcgaaaat ccggagagct ttcgagaggt gaagattccg
     2401 ggcatcccgc acgcgggtat cctgacctcg gcgatgtttc tgaatcggtt tccgacgagc
     2461 gacacgaaca tcaaccggca ccgttcgcga atgacgtact ggttcttcct cgacatcgac
     2521 atcaaccagt tcggtagccg gccggtggat gcgagcaacg acttcggaga gaacccgacg
     2581 ctcaacaatc ccgactgcac ggtttgccat acgacgatgg atccggtcgc aggcctctac
     2641 atgaactgga acgagagtgg cgagtacagc ggcaagggtg aatggttcga cgcggactac
     2701 attctgcccc ctggcttcaa cggagaggcg ctcccagcag agagtcgaag gaacgcactt
     2761 cagtggtttg cggagcgcgt tgctctcaat ccaaggttcg cacaggcaac cgtcaagacg
     2821 gtgtttcagg ggctgaccgg acagaagatt ctggacatca gcgttccgat gacgccggag
     2881 atggcgagtg atgccatgca ggaacccgat cccatggtgg atcccgatcc catggtcgac
     2941 cctgctgccg ggatggatgc gggcgtcggt ggcatgggcg gcatgggtgg catcggtggt
     3001 gccgcgggcc tcggtggcat gggcggcacc acgccgcctc ctccgcctcc gccacctctt
     3061 ccagaggtcg atccggaaat cgacccggac gtcaatccag cgctcgagcg cgcctacaac
     3121 gtgcagcagc agatcctgcg aacgattcag agacgcttca tcgaaagcgg ccacaacttc
     3181 aagctcttgg tgctcgagat cgtcctcagc ccctacttcc gcgccaagaa cgcaagccct
     3241 ctgaccagcg aaatggagat cgagctttcg acctttggca cggcgaggtt gctcacgccc
     3301 gagcatctct cgcgcaagat cgaagccaca acgggcgtgc gctggcgcgc gcgccctgat
     3361 cgcaccgact atctcctcga tcagtaccgt atcttctacg gtggcatcga ctcggatctc
     3421 gtgacgcaac gcgtgaccga gccgaacggc gtcatggcaa gccttgccca gcggatggca
     3481 tacgaggtcg cctgcctcgc agttccatac gatttctcga aacccgccaa ccaacgtgtc
     3541 cttttcccga acatcgatcg gaacgctccg ctttccgaag catccgatca ggttcgtgac
     3601 acggttcggt atctacatga gcgaatcctg ggcgagtcgc tcgcgccaac ggacgaggaa
     3661 gtcgaggcca ccatggtcgt gctcatggaa gcctacagcc ttggacgcca aggcctcgcg
     3721 gacgggtccg tgcccgagga tctcgcgggt ccatgccgcc tcaacgaaga tcgaatcacc
     3781 ggcgagtcat tgccggacga gcgccgaatc aaccgggaca ccgaatacac cgtgcgcgcc
     3841 tggatggctg cggtgagtta tctcttgtcc gattaccggt acctgtacga gtgagggagg
     3901 actagctgtg gaacgtcgtg actttttacg aatcgcaagc tgggccggac tgacctgcgt
     3961 cttgccggca gtgatggacc ctctgcgcag tagcgcacaa tcttccggat acacgggcac
     4021 gtactggatc ttcatgcacg ccggcggcgg ctgggatccc acacatctct gcgaccccaa
     4081 gggtcaggcg accgccaacg agaacaaccc gatgggacgc tttctcaaga gcgacatccg
     4141 gactgctggc aatatctcgt atgcgcccct cggcgacgac ggaaccgccc tcgacgcgtt
     4201 ctttcagacg cactatcagc gcctgctcgt gatcaacggc atggacacga ccaccaacgg
     4261 tcatgaccag gggacgcgcg gaacctggag cggtcggacg gccgagggcc atccgcccat
     4321 cggagccctg atcgccgcga cgcacggccc gaacctgccg atgccgctca tcgggtacgg
     4381 tggatatctc gacactcagg gcgtcacgaa cgctgtgccg ctcggcgacg gcggtctgct
     4441 ccacaatctc gcacatccgt ctcgcccgga tccgaccggc tcggacgaga actacttcgg
     4501 tcatccggac gtgcccagca tcgtccaacg tgagcagcag ctccgcctct tgcggctgca
     4561 aggaaagcaa cgcctgccgc gcatctggga tgggcagaat gagctcatgt tggctcgtct
     4621 cggacaggac gacctcaagc gtgtgtcgga ctcgctgccg gagaacttct ccaacaatca
     4681 gctcgagcgc caaattcaag tcgctctggc cgcgtatcag gcaggtctct cggtgagcgt
     4741 gagcgtgagc atgggtggct tcgatacgca cggcaaccac gacaacaatc accttccgcg
     4801 cctcatcgac ttcgtcaacg gcgcgaactt cgcgatgcaa gaagccgaac ggatgggcat
     4861 cggcgacaag gtggcggtcg tgatgggctc ggacttcggg cgtacgcccg ggtacaacaa
     4921 cggcaatggc aaggaccatt ggccgatcag cagcatgatg atgatgggtc ctgggattcc
     4981 cgggaacagg gtcatcggcg gcaccgacga tcgccacggt ctgcagctca tcgacccggt
     5041 gacccttcag gccaccggct cgcccgacag cgcccagggc acccggcttt cgatcgcgca
     5101 cgtgcaccgc gcgctgcgca acctctcggg tgtgcccgac cagtacaagg cggcgttccc
     5161 gctcgcgggt gaggacctac cgatcttcga ctaagtcagc gaccagagga cgagccctcc
     5221 gatcgccgtg gcaagcaggg accagagccg catcacgaag ggcgagtggc cgatccacca
     5281 gtcgagcacg cgttcgaagc gctcatagcc gatgattgga aggcccagcc ctagcaggat
     5341 cgccaaggct ccgagcgctt ggagaagcac cggcgatcgc gtgtcgggcg cggcgaacca
     5401 tagggcgagc ccgaagcaga gacggagcac gaccgcaagc cagaacccgg tcttcgagct
     5461 ccaacccgag atgaaggcga agatcgaccc tggggcaaaa accccccatg cgcccatcac
     5521 gaagatgaag agcgccacag caagcgcgag cagcacaaac atatccgacc cagcctcggg
     5581 accgtaaacc ggccctgcgg cggcgggacg atatgccgag tctcttccga gcgcaacgcc
     5641 cgacgctcgg tggctcagtg cctcacgggc tcagaacgag gcgccgcctg cgatgcgcag
     5701 gccccagacg cggcgcgaat cgaacgagaa gccgttggga ttgtcgatgt tgatgttgag
     5761 cagcaaagct ccgaatgcgt tttcattgcc gaatcgcatg aagggctgaa ccgcgagctg
     5821 cgtcttctgt tcttggtatt tcggaatcaa caccaagctg aaatcggagc caactctgag
     5881 ccagtcggtt gcctggtagc ccatcatggc ggccatctgc atgatgaagt cggtcttgga
     5941 ggtcctcggg ttcttgtcgt tgagcttgat gagggcgccc gtgtcgagat aggcacccca
     6001 tagaaagccg ctcggcttac gccgctcgaa tgcgatcgga acgatgacac tcaccgtatg
     6061 cgggtcccag agccaaaaca tccggttgcc acgcatggcg gcggcatcct tgtacgcggt
     6121 ggcggccgta acctgctcat tgatatcatc gggaagcgtc gccgccggaa tggtcgcccc
     6181 gaccccgacc cggtagctga actggttctt cttgccctgg tagtggaacg ccacgtacgg
     6241 gtttccgaag cgaaaactgt tgtcggagcc gccgtcctcg aggttgaggt tcgcgtagga
     6301 cattccccac acgacctgca tcagaaagct gtacgccaac tgaagacgca tctcgatcaa
     6361 cgggctaatt acgacctgct tctggtccgc ctcgcccttg cctgcgtaga cgccgatctc
     6421 tcccaaaatg tggaaccggt cggtgtgcgc tttttcgccc tcgtccaggc tgaacggctc
     6481 gtcgggatac gcacgcgcct caccgggcgc cagccacatc ataccagcca gcaacaaacc
     6541 aatcgctccc caaccacgaa gaaaagacat caactcaccc ctcaggaaaa agagaccccc
     6601 ctccttttct ctcgatcagt ataagcagcc gcgagagccg gggcccatgt tccgtcttca
     6661 acggaactcg tgtctgaggt gtgttcgcgt gttcagggtt ggacggcgac gtacgacggg
     6721 cccggctctg cgagccaatc aatcgcccgc acagcattgc tcgcgagatc gataatcgag
     6781 agcttgcggt cgacgatgtg ggcgacgaac gcgaagtcgc acgcggaatc gatcgccgca
     6841 gtcaggggga agtttccacc cgaaagcggg agcggtggac cggccgataa ggattctgcg
     6901 ctcacggtga aagtgaccaa ttgagtgggc agatcgcgcc cggtcgcatt ggtcggcgcg
     6961 aggatctggt ttcgcaccgc acaataggtg acaccgtagg gaacgccgac gccgacggga
     7021 acgctctcga tcacgctcgg actgctcggg ttcgaggtat cgacgagcgt gacgtcaaag
     7081 ccgaatgagt tggcaacgac cgcgcgatcg ttaccgtcca agaacgacac gcccgacgga
     7141 tccgaacccg tttcgaggac ttcactgagc gttcccgcga cgtcggaggt ctggaaaatt
     7201 cgaatgccac ctccactcac gacgttgacg atgccgagac ttccatcggg actcagcgcg
     7261 acctgttccg gaccgcttcc gaccgggaca tcttcgatca gctgcctgcc cgggatgtcg
     7321 atgactgaaa gcgtgtcacc tcgagcgtcg gccgtaccgt agttcgcggt gtaagctcgg
     7381 cgtccgtccg gactgatcgc gattccgagt ggcacgtcct cggtttggat ttcgtctgcc
     7441 gttccggtct ccaagtcgac gatgagcaag gtcccgccgg gagggatctc cggatcgccg
     7501 agaagcccag acagcgaatc gaaaaacgcg ggacctaccg aaaccaccgc cgtcttaccg
     7561 tcgggtgtga tctcgagctc gatagggccg ggttcccaat ttgagaggtc gatcgtatcg
     7621 acgatcgctt gggaggcatc gctctgcccg tcgaccaact tgtcatagtc gaagagcgtc
     7681 aacgactgat tcaaccaatc ggcagtcacc acgagtcggg gagctccacc ggctccaccg
     7741 atgccgcccg ttccggcaga accgccagta cccgctgcag actcgctcga tgagtcacaa
     7801 cccaagagga cgagcgagaa accgaggagc acgaggattg tacgagaggc gcgatgtagc
     7861 atcggaggag tatacgccaa tcgtaccgat gctcagccga gattttgttg cgcgccggcc
     7921 ggatctgccg tcagagccgc atccagatct tcatagcgat gcaggagtgt ggccggaagc
     7981 cgatagctcg ccagctcgtg ggttgcggga aggcctaacc aaacttggtg gctgcgagcg
     8041 agcaactcga ttccggctac gagttgctgg gactcgtcgg gctcgcagtc acgcgcgatg
     8101 ctcaacagga tgagcttcgc cccactttgc ttggcggcct cgctgatctc tgagaagggc
     8161 atgtgcgtgc cgagataaat cggttcgaag ccgcggctcg ctgccatgaa cgcaccgatg
     8221 atggcgccaa actcgtgcag gtgacgggga ggcgtggcca cgatgactct cggcgccccc
     8281 gtgaccggct ccgcgtgccg catcaaggtc cccagcaagc ttcgaagctg cccggaaatg
     8341 atgtgctcgt gggcgatgcg aagttggtcg gagttccatg cctcgccgac tcgccgcatc
     8401 aggggaacga ggacttccag cgtgagcgtc atcggagata gaatcgcggc cgtccgagtc
     8461 agcatcgact ccgcacgctg cacgtcgtag tcgaagatgg cttcgaggta gtcctccacg
     8521 accgccgcat aaagagagac cgtgctgctg acgtccgggc cggtctcacc gatcagtcgg
     8581 cgcacttctt catccggaag gctcgcgatc tgaccgatcg agtgcccgag ctctgtggcg
     8641 cgccgcagca ggaccaggcg acggacgtca ttcccggtgt atctgcgctt gttgccctcg
     8701 gtgcggtccg gatcgaccac gccatagcgg cgttcccaga cgcggatcgt gtccgcagtt
     8761 aggcctgtta gacgcgctgc aactcgcatc gggtagaagc cgctgatttc ttttacgttg
     8821 ttggtcatga tcaaagtggc acagggcgag cacccacggc acaagtcacg ctgaatcgcc
     8881 tgacgatgaa tggggtggtg gtgctgcatc catcttgcac gctcctgcag tgagtacccg
     8941 ctctgcaccg ggaaatgaga atgaggacgg agctcgggag aggcccgacc tcggttcgct
     9001 taggaagagg gaatgaggac ggtgtcgatc acgtgaatga ctccattgga tgccatcacg
     9061 tcggtcttga tgacgtttgc atcgttgatg cgaacaccgg cgttgcccgc cgtgatcgtc
     9121 acgggttgac cctcgacggt atccgccgag gagagcttga ccacatccgc ggcgagtact
     9181 tcgcccgcaa cgacgtggta cttcaggatc gccgtgagct tggccttgtt ctccggcaag
     9241 aggagactgt ccacggtccc tgccggaagc ttcgcgaacg cctcgtcggt gggcgcgaag
     9301 acggtgaacg ggccagggct ctgaagggtt tccaccagat cagcggcagt tagcgcagca
     9361 accagcgtgc tgaattgacc ggagccggcc gcgacagcga cgatgttcgg ctggttgatg
     9421 cgggccgcgg tgctggcggc ggtgctggcg gcggtgctcg gagcgtcctt tgcgcaaccg
     9481 agtagtgcga tagaagccaa ggctgagatg agtatcgatt tcgtttgcat taactagtcc
     9541 tccaagagtg tttgagaagg caagaccttc tgagagtcga ctgcgggttc gccaacggcc
     9601 gcgtagcgac cgagatgcgc cgcaaaacgc cccatcaacc aacggtgaaa tacggcctgc
     9661 gtgctcacat agacgagcca ggggagcctc ggctcatagt cgtgcacgac ggtgagcagg
     9721 gtgttccctc cgagcacctg ccggagctcg aacctgggtt tgccgccttc tcgcgacaga
     9781 agtcccccag tgacatagag gagctgccgg tcagagccgc tgcggtcgga ggcgcggctg
     9841 agctggagca agctcgctcg aaaaggccag aggacgaagc tgaatcgctc tggcgagtcg
     9901 agctcgacgc gcacgagccc gttgagaaag cgaggcaacc agtcgaggta ctcggcagcc
     9961 gcccgcaggg ccgtccagcc ctttggcaat cgcattcgct gcacggagcg aaccaggcgt
    10021 tcacgagaaa caaagcctcg aaaggcatgt ggaaccgttc cgtctgactc tgcaagcgct
    10081 tgacgaatcg cgtcccgcgt cgacgtgcgc gagatgccga gctcttctgc cagcccgtgg
    10141 tcgcgaacga ccatcttgtg cttgaggctc tgtacgaggg gtgcaaccag cgacttcggg
    10201 gcgcccgtga tgagggtgac ccagagtctc gaaagaccag gcgacatgaa gcgagatgga
    10261 atgaacaatc gccgttttcc gagctcggat gcgatcatcg ccatcatctg cttgtacgtg
    10321 acgacgtctg gcgcaccgat gtcgtatgtc cgtccaaaat gctcttgccg acccaccact
    10381 ccgctgatga attgaatgac gtccgcgagt gcgacgggct gtgtttcgat gttcgtccag
    10441 cgtggacaga ccatgacggg caagcgctcg acgagtcgcg ccatgagctg aaacgacgaa
    10501 ccctgtcctc ccaagatcag ccccgcacga agggtggtga cgggaacccc gtgttcgccg
    10561 agcgccttct ccacttcgca acgactctgc agatgggaag aaagtctgcc ggattcaggg
    10621 gtgagaccgc cgaggaacac gatctgcttc acgcccgcgc ctgcggcggc cctcgcgaag
    10681 ttgtccgcgc aaacgaggtc caggtctgcg aaatcacctt gggtgagacg tgccgatggc
    10741 atcatgttgt gaacgagata gaccgcgtat tctgcccccc ggagcgcgtc ctcggcgtct
    10801 ttcagtgaga acaggtcgca gacgcggaac tcgtcgaaac ccccgccggg ctcacgctcc
    10861 gtacgggaaa gcccgatgac ctcgaaggct gcggacaacg caggggctag tgaacggccg
    10921 acgaacccgg acgctcctgc gatgacgact ttcggcctgg gcccggaaac catacttgtc
    10981 tatctaatgt ctaagttatt atggtcaata cctagacacc aatttcaagg cagcccgcca
    11041 aagcgatctc ttgccttggc atgagaaatg aaacatgttc tagtgaaaca cgttctagtt
    11101 ctcgcccgac gctcgggtcg ctgcgaaacg attggagccc tcgaatgacg aatgaaaaaa
    11161 agacgaatcc ctgggcaggc tttgcacggg gctggttctt actgatgttc tccgacgagc
    11221 tagcgcccgg gcaagtcgtg ccgctgaagc gcttcgatca ggagctcgtc ttgttcagaa
    11281 cggagtcggg cgaagccaag gtgctcgacg cgtactgtcc tcacatgggc gcgcatctcg
    11341 gacatggcgg caagatcgaa ggcgagggaa tcgtctgccc tttccatgcc tggaagttcg
    11401 acggcagcgg caagtgcatc gagattccgt atgcgaagcg gattcccccg aaggcaggcc
    11461 tcgcgtgttg gcctgttctc gagcgcaacg ggatgatttt cgtttggaac gaccaggcga
    11521 agagcgcacc cgactgggag atcccggtgc tcgaagagtg ggacgatccg gagttcctcc
    11581 cgtggcggta cgcaaagctc cagatcaaga cgccgccgct cgcgatcgca gagaacctgg
    11641 cggacgccgg tcacttcatt ccggttcaca acacgcatcc gatccctggc tcgttcagca
    11701 acacgtacga cgcccacgta ggacagcaga agatcaaggc gattgcttac cccgttcatg
    11761 gaggcaagga cgaattcgag tcgaccgcga cctattatgg acccgcgtat caggtcacca
    11821 agatgaaggg aaagggcctc ggcgtgatca tcaacgcgca cactccgatc gacgacgagc
    11881 acgtcatgct ccgattcgga gtctcgatga aggcgaccaa gggcatgacc gacgagctca
    11941 tcgagcattt catcaacaac atgcgagacg gctatcttca agacgtacag atctgggaaa
    12001 acaagatcta cctcgaccgg ccgattctct gtgatggcga tggaccgatc ggcgagttcc
    12061 gccgctggta cgcacagttc tacactccac gcaacgaagc ggccgacgcc gccgagtagc
    12121 gaaggcgagg cgcatgagta tgttgaccaa gcccgatttc gtgaagctcg cgcgtgacgc
    12181 gaagtcacga ctacgcgagc gcaactcaag ggccgatgcc gagcgcgtca tcccgaaaga
    12241 gacgatcgag gagctcaaag aagccggttt ctttcggatg atgcagccaa agcgctgggg
    12301 tgggtacgag tgccaccctc tggagttctt cgatgcggcg atcgaaatcg cgtcagcctg
    12361 cccgtcgact gcctgggtgc tcggcgtggt cgcggtgcac aactggcagc tcgcgctgtt
    12421 ccccgagcaa gctcagaaag acgtttggga aggccagaat ggtctggata cgctcatcgc
    12481 gtcttcgtac atgcccgttg gaaaggtgac gccggacgac ggtggatatc gattgagcgg
    12541 caagtgggga ttctcgagcg gctccgatca ttgccaatgg gcttttctgg gcgcgtttgc
    12601 tcccaaggaa gagggccgcc cacccgacat gttcaccttg ctgctgccgc gctccgacta
    12661 cgagctgatc gatgattggt tcgtttcagg tctcaagggc acggggagca agtcggtgaa
    12721 ggtcgacgac gtgttcgtcc ctgcgcaccg ggtgcatcga atgtcggacg cgtaccggct
    12781 tcaaagcccc ggcaatgacg tcaacacgag ccccctctac cgggtccctt ttggtcaact
    12841 cttcacgcgc acggtggcga cgccggccat tggcatgctt cagggtgcgc tcgaggccta
    12901 cttggccctc aaccgaggtc gaaaaggccg cgccgatgga aagagcgcgt cggacgatcc
    12961 cttcaacctc gagacttgcg cccgcgcaac actggcgatt cgtgaggtgc gcgcgctact
    13021 tcgcaacgac tggaacgaga tgatgtcgaa ggcggaagcc ggtgaaaaga tcgaggtcga
    13081 agatcgggtc gcatatcgcg caaatgccgg cgcggcgacg gatcgcatgg tcaaggcgct
    13141 ggacgagctc ttcatggcga gcggaggctc ggcgatcttt ctcgaccaag acattcagcg
    13201 cttctttcag gacatccacg gggcgcgcgc ccatcacgcc aacaatccgc tcaaaaccca
    13261 acgcaactac ggcggcctct tgatgggcga gcaaacgacg gacttctttc tatgaggcgc
    13321 aaagacggat tacgagcaat gggagaaaag ccatgagcaa gaaaccacga tttccgatcc
    13381 cccgatatcc tcgcgggtgg ttccaggtag cgtactccga cgagatcgcg gccggtgaag
    13441 tcaaagcgct caagtacttc ggggtcgatc tcgtcatgtt ccgcacggaa gacggtgagg
    13501 tcaacgtgct agacgcgtac tgcccccaca tgggcgcgca cttgggacac ggcggaaaag
    13561 tcgtaggcga tggaatcgtc tgtcctttcc acgcctggaa gttcaacggc gctggcaagt
    13621 gcatcgaggt tccctacgcg gagcatcttc cgcgcaaggc gaacatcacc gcatggccgg
    13681 ttcacgaggt caatggcctg gtgatggtct ggcacgacat cgaggggaag gcgcccgcct
    13741 gggagatacc gacgatcccc gagtggaaca acgaggactg gactccgtac tggcgccagc
    13801 actggaagat tcgcacacac aaccaggaca tgtgcgagaa cgtggtcgac aaggcccact
    13861 ttcgatacgt gcacgggatg aacatcgtgc cgcagccgca cacgatcaat ctagagtacc
    13921 cgcacatcaa gatgatcacc gatacgatct acgaaacgcc acaaggtgag gtcaaaggcg
    13981 agctcgaagt cgacgtgcat ggtttcggct tctcgctcag ccgatttcga ggaatcgtgg
    14041 aaacgaccaa catggcctcc gtcacgccga tcgacgaaga gcactgtgac gtgcgcttct
    14101 catttaccgt gaagaagata ggcggggccg acatcacagc cggggtaggt cgggcgttca
    14161 ccaaggaaat cgcgcgccag ctcgaggagg acaccccgat ttgggagcat aaggcattct
    14221 tcgagcgtcc aatgctctgc gacggggatg ggccggtcgc ggagttccgc aagtggtgca
    14281 agcacttcta ccccgactgg taccacaagc aggctcaaga cgagtatgat ggcgtcaagg
    14341 aggtcagtaa gcccctcacg ctagcagagc gacgtcgacg gcttgcggca gcatagatgg
    14401 acagaggttc tgaacgaaga agtccggcaa cgcccgatga aatccgggcg cgtatccgga
    14461 gcgagcacgc tgcgatctcc gctcagcttg cgcgcgtcga agcactcacc gaacgcatcg
    14521 gtgatgatga ccgcgaatcc gtgatcgcgc tgcgcgacga gttgcaggtg ctcggcgaga
    14581 tcttgctcga gcatttgcac tacgaggagt accaacttcc ctcattggcg ggcacgagcg
    14641 aggtcgccca aatggtcgaa gacatgggcg aggagcaccg agcgcagcgc gagatcttcg
    14701 acagcgtcat ccgtgagctc gacgagacag ccgtggggtc gaagttggta gtgggtgtgc
    14761 gcgagctctc acgcgcgatc cgcgacgaca tggaacacga ggagcgagag ctgctgaaca
    14821 aaccctgatt ccggagccga gaaatgaagc tcatgtacaa cccggcgacc tgcaatcact
    14881 acggcgtctg cgtcgaagag gccccggagg tcttttcttt cgcagctgac ggaagtctgt
    14941 cgatccaaag ctacgacgtt cctcccgacc tcgaggtcaa ggtccgtgac gcctgcatga
    15001 tgtgcccgac gcaatcgatt cagatcgaag aagagtagag tcgaccagcc gtaccgctca
    15061 ggactcgaaa gcgcagcgtc ccccgttcag cccttgcgct tccgagtcgg gtcctcgtct
    15121 tccttcagct ccttgtagtc gatccggaat cggccctcgc cgagtgtcgc aatcgtttcg
    15181 agcgcttgga ggcgagcctc cgtgtcttgc ttgtacgcgc gcaactcgct tagctcctct
    15241 tgcatccgct ccgcctgttc ggcgcgcgcg aggcgaagcc ggtaccagag ccagagcccc
    15301 caaccgacca gtcccaggat cgcaagggga atgatcacct cgatgatgac agccactggg
    15361 atcttgtgcc ctcacctcgc ctggaccgca agttaatccg gagcctgcat gctatacaga
    15421 acgcatgagc ggtgaggtcg acgaaatgaa cgcatttcgc gagctgcaat cccagctcgg
    15481 gcctcgctgg gcgctggccg acggagacgg atcggagccc cgggacatcg tcgtcgtccc
    15541 ttcgttgtcg ttcgacggca tcgacacggt ctcaattgat ggtgttcttc actacgaaga
    15601 gcgaatgctc ttcgcattga atctgctgcg gcaccctcga gcgcgcctgg tctatctcag
    15661 ctccgcaccg attcacccgg cggccgtcga ctacatgctt tcgctcatca gcggcattcc
    15721 gaacgaacac atgcggcgcc gactgaccgt gctctctctc tacgacaact ctctcgtgcc
    15781 cttgtcacag aagatcctgc aaaggccgcg cgtcatcgaa cggctgcgtc agctcattcg
    15841 ccctgaatgt gggctgatga cctgcttcac cgtctccgaa ttggaacgcg atctcgcggt
    15901 tgcgctcggc gttcctctct acggcctcga cccggatttg gcatggctcg gcaccaagag
    15961 cggttcacgc tgggcgttcc gaaaggccgg aatccctctg ctgcctggcg tcgaggatct
    16021 gcggaccgaa gaggatctcg tgcaggcgat caactcgctc cgggacgaga atgagggtct
    16081 tcgccgcgta gtcgtcaagc agaactacgg cttcggtggt cagggcaacg caattctcga
    16141 gctcggtgct ctcgattcgg cttcgcgcaa cgaggatctg aaggcagcgc ttccgaacat
    16201 gcgattcgtc ggtcagcggc agacctggac gagtttcatg gaaaaattcg agcgcgaggg
    16261 cggtatcgtc gaggccttct tgagcgatga gagcaagcgc gccccgagct gccagttgcg
    16321 aatctctccg gagggaagct tggacgcgct ctcgacccat gatcagctcg tcgacgggat
    16381 cgatggtcag atctacgccg gctgccggtt cccctctgat gccgcctatc gggcggacat
    16441 tcagaaggat gcgctgcaag tcgcagcggt tctccaggag cgaggtgcgg tagggcgcct
    16501 cgcagtcgat ttcctatgcg tgcgggccga tgacggtacc tggcgccgct atgcgatcga
    16561 aaccaatttg cgcctgggag gcacgacgca cccgatgatg ctcctgcgca tgatgaccga
    16621 cggccgatac gatgcggaca ccggtctcta cctcaccccg cgaggcgagc cacaattcta
    16681 cgtcgccacc gattcgatgc aatcggacag ctaccggggt ctgctggtcg aggacctgct
    16741 cgatattgca gcggtgaacg gtttgcacta tcaggcctgg tccgaatcgg gtgtactctt
    16801 tcacttcacc ggcgcgctca gcgagttcgg gaagctcggc atcaccgcag tgggcaagag
    16861 cgctgccgag gcggaccact ggttcgaacg aacccgtctg gtattggacc gcgaaacgaa
    16921 gagtcaccct tgcacatcga agaagtagga cctggcgtat tgcgggtttc gccctccgcg
    16981 gattggcccc gccccta
//
LOCUS       DASRZF010000385         3037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_5102071, whole genome shotgun sequence.
ACCESSION   DASRZF010000385 DASRZF010000000
VERSION     DASRZF010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5102071"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VFG22_17150"
     CDS             <1..182
                     /locus_tag="VFG22_17150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418025.1"
                     /translation="AASKGQRVQQEDKACRGNKPKPRSSDGGGEGNQTFEAHRRTVTA
                     GGKRVIGPQLQVNAK"
     gene            516..1835
                     /gene="ltrA"
                     /locus_tag="VFG22_17155"
     CDS             516..1835
                     /gene="ltrA"
                     /locus_tag="VFG22_17155"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HET6418026.1"
                     /translation="MTKPSISLQELRKRMYLKAKAEPNHRFWGLYVHVCKMETLREAY
                     RNAKANNGAPGIDGVTFEDVERSGAEALIEQLRDELTSRTYRPLRNRIHEIPKGDGRT
                     RRLGIPAIRDRVVQGALKLILEPIFEADFQDGSFGYRPKRSAHQAVQRVATAIAGGKT
                     HAIDLDLKSYFDTIPHGGLLAKVAKRVNDDDVMHLLKLILKAGGKRGVPQGGVISPLL
                     SNLYLNEVDEMLERARRTTMGANGWDRVQYARFADDLVILVDGHRRWEWLRVAVYRRT
                     LEELAKLDVRVNVEKSRRVDLTEAHASFTFMGFEFRRVRGGSGKWRPSFAPRAKSRTA
                     LLEKLKGVFRMHVSHPIRLVIAKINPILRGWVNYFRVGNSSRCFSYVRQWIERKIRRH
                     LMRARHRNGFGWQRWSKAWLYYALGLHADYAIRRMPRLAARPAEVAT"
     gene            2170..2985
                     /locus_tag="VFG22_17160"
     CDS             2170..2985
                     /locus_tag="VFG22_17160"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET6418027.1"
                     /translation="MPRANRYIVEGHAYHITHRCHDRSFLLRFGLDRDEYRRRLRAEL
                     KGSGVWLLAYCITSNHVHMLVTARTLERLSRFMQKLQGEFAEWYNVRRKRSNAFWGDR
                     YHCTMIESGTHLWNCMAYIDLNMVRAGEVKHPREWRWGGYDELVGERTRHTVLDMGRV
                     LEAAGAGSRDEFVRDYGAAIEEMLGRGDAGRQPHWTESIAVGSREYVTGISERIGGRT
                     RIKFREVVDGAWSVREPGAASGADATEDAEQPYGRFRGTKIERKRGFWRLDWA"
BASE COUNT          640 a          861 c         1032 g          504 t
ORIGIN      
        1 gcgcggccag caaaggccag cgcgttcagc aggaagataa ggcctgccgg ggcaacaagc
       61 cgaagccccg tagctcggat ggaggagggg agggaaacca aactttcgaa gcccatcgac
      121 gaactgtcac cgcgggtggc aagcgagtca tcgggccgca actacaagtg aatgcgaagt
      181 agcctcgaaa acccaagaac cgagggccga gcgctttggt ccggtgcgaa ggcagtagga
      241 acggagctga ccggctaccg tctgttcccc tcggcggggt gtttgcagcg gcacgttgac
      301 aaggacgcgt tgggaactgg cgaagccctc ggagccccga cggagaaatc tgcggaagcg
      361 aggtaggcga ctataaccgg agcccccggg aaatgacgcc gacggctcaa gggtgacgga
      421 cgggcccgta gtagcgatga agcggagtaa tgtccgtgga gcgaaggggc cctgctgacg
      481 acgtttcgaa tccaaaacgg aaaggcaggg gcggcatgac aaaaccgtct atcagtctgc
      541 aagaactgag gaaacggatg tacctcaagg cgaaggctga accgaaccat cggttctggg
      601 gtctgtacgt tcacgtctgc aagatggaga cgttgcgcga ggcgtaccgc aacgcgaaag
      661 ccaacaacgg agcgccgggc attgacggcg tgacgttcga ggatgtcgag cgatcgggag
      721 ccgaggcact catcgaacag ctccgtgacg aactgacctc acgcacgtat cggccactac
      781 gcaatcgcat ccacgagatc cccaaaggcg acggccgcac gcgtcgactt gggattccag
      841 cgatacgtga tcgcgtagtc cagggagcgt tgaagctcat cctggagccg atcttcgaag
      901 ccgactttca ggatggctcg ttcggttacc ggccgaaacg tagcgctcat caggcagtgc
      961 aacgcgtggc cacggcgatc gcgggaggca agacgcatgc gatcgatctg gacctcaagt
     1021 catacttcga cacaattcca cacggcggcc tcttggccaa agtggcgaag cgcgtcaacg
     1081 atgacgacgt gatgcatctg ctcaagctga tactcaaagc tggaggcaag cggggagttc
     1141 cgcagggcgg agtgatctca ccactgctca gtaatctcta tcttaatgag gtagacgaga
     1201 tgctcgagcg agcgcgccgc acgaccatgg gcgccaatgg ctgggatcgc gttcagtatg
     1261 cccgctttgc ggacgacctg gtgattctgg tcgatgggca tcggcgatgg gagtggctgc
     1321 gggtggccgt gtaccggcgc acactggaag agctggcaaa gctcgatgta cgagtgaacg
     1381 tagagaagtc acgcagagtt gatctcaccg aagcgcacgc atcgttcaca tttatgggat
     1441 tcgagttcag gcgagtgcga ggcggcagtg gcaagtggcg tccgtccttt gcgccacgcg
     1501 ccaagtcgcg cacggccctg ttggagaaac tcaagggtgt gttccgcatg catgtgtctc
     1561 atcccattcg cctcgtcatc gccaagatca atccgatctt gcgtggctgg gtgaattact
     1621 tccgagtggg caattcgtcg cgctgcttct cctacgttcg tcagtggatc gagcgcaaga
     1681 tccggcggca cctgatgcgc gcgcgacacc gcaatggatt cggctggcaa cggtggagca
     1741 aggcgtggtt gtactatgcg ctcggattgc atgctgacta cgcgatccgg cgtatgccac
     1801 gtcttgcagc acgaccggcc gaggtcgcca cataaccctt gaagtgaagt tgtcaggtga
     1861 gcgtagtgcg ggaaatccgc acgctgcgtt cgaagtggcg gggactggaa acggagtcat
     1921 ggtgatgctc gtgaggcact cccaacggaa acggggagta acagataggc acacctacgg
     1981 ccaccgcgcc agtcctcgac cctaccagga tgtgcttgtc cccacaggac ttgacccgga
     2041 atctcgaaca gaaaccccgc catacgctcg attttgggcc caacaaatcg ggcgtatggc
     2101 gttcggcgct cgagacgcac ggcgggcggc cttgccgcgc tgcgccggag cggtatgctg
     2161 ggcgcgccca tgccgagagc gaatcgctac atcgtcgaag gacacgcgta ccacatcacg
     2221 caccggtgcc atgaccggtc gtttctgctg aggttcggcc tggaccgtga cgagtaccgc
     2281 aggaggctgc gcgccgagct gaagggctcg ggcgtttggc tgctggcgta ctgcatcacg
     2341 tcgaaccacg tgcacatgct cgtgacggcg cgcacgcttg agaggctgtc tcgcttcatg
     2401 cagaagctcc agggcgagtt cgcggagtgg tacaacgtgc gcaggaagcg cagcaacgcg
     2461 ttctggggcg accgctacca ctgcaccatg atcgagagcg gcacgcacct gtggaactgc
     2521 atggcgtaca tcgatctgaa catggtgcgc gccggcgagg tgaagcaccc gcgcgagtgg
     2581 cggtggggcg gctacgacga gctggtcggc gagcgcacgc ggcacaccgt gctcgacatg
     2641 gggagggtgc tggaggcagc gggcgccggc agccgggacg agttcgtgcg cgactatggc
     2701 gcggcgatcg aagagatgct gggcaggggc gatgcggggc gtcagccgca ctggaccgag
     2761 tcgattgccg tcggaagccg ggagtatgtc acaggcatct ccgagcggat cggcggcagg
     2821 acgcgcatca agttccggga ggtcgtcgac ggcgcgtggt ccgtccgcga acccggcgcg
     2881 gcatccggcg ccgacgcgac ggaggacgcc gagcagccat acggccgatt tcggggcacc
     2941 aagatcgagc gcaagagggg gttctggagg ctggattggg cctaacctct gtcagtgtaa
     3001 ggagatcttg tggtccgacc ccagaacacc ctgcgca
//
LOCUS       DASRZF010000386         4661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2358394, whole genome shotgun sequence.
ACCESSION   DASRZF010000386 DASRZF010000000
VERSION     DASRZF010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 4661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4661
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2358394"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VFG22_17165"
     CDS             <1..264
                     /locus_tag="VFG22_17165"
                     /inference="COORDINATES: protein motif:HMM:NF024687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HET6418028.1"
                     /translation="VQVTWADDVEVDLPVSLRIMTDDRAGILASVSSVFSANGVSISE
                     ASCLSRDGRAENMFHFRVGDIDRLREIMRGVAKLKGVLDVERV"
     gene            complement(261..1094)
                     /locus_tag="VFG22_17170"
     CDS             complement(261..1094)
                     /locus_tag="VFG22_17170"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HET6418029.1"
                     /translation="MGGYLRALLIALAVPALNHLFLGLAGFLQLRSLSASSHEAIRAA
                     AQAPLNLALVQAASVGLIFIIAFPYKKREDGLLESVNVLPLAGGIVALSFLAGALMQI
                     PLAELGNLVQEVWPVSFEELARRHRLVNPTSWWGGSSVLLALVLVAPLTEELLFRGWL
                     LQDLKDQYGQRKALLWSSLLFGLVHLELSAILYATLGGLVLGAVALRTKSTLASIAMH
                     AGVNALPLLLPASLLRIEGFNTLSQRVEHVSVWLLLLSSGALVALLAILWRATEDASR
                     A"
     gene            1185..3302
                     /locus_tag="VFG22_17175"
     CDS             1185..3302
                     /locus_tag="VFG22_17175"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6418030.1"
                     /translation="MRIRFLPILGLLTLTVACAHSKSATVGSRVGSAEPIPQAEYPDE
                     ARRFMRMSLDDPERVGLRDRLVASVLSQAQSLAEVDDYEGVVSEVSELTTFLSPHDYA
                     NGILPKSVEPIAQYMAEEGGHRGDEGNVLAANFLLERLTGDEIYQQRYETVASWGHES
                     RANLDTVSEQYAGLIAVWNEHAELTPAPKVLDTLADLYIAGRDAVSRETVETRHRLGI
                     GGERQLRLAPLNVAAVYLSHGDIESAVSKVGTMGSGGELQSRLLDLLTKAQSADEAGA
                     AATFELVEGYRLARSDVAAGLCRSALLRFPKDERFPTCMARVAADSEQFADATAWYVI
                     AIELDPESIDLYDEALGQLDEFIETRLSDPHPERSSALAHGAETILEERQKRWPDSKP
                     PIAPNELEFLIGMLEMNAGHVVEAQQRFETSLAKDENVGALLQLGLLFERTGRPLEAE
                     QRYRRALELAPSDTLAEDVRRAEILENLGNAARAQGKTREMTTHYKSALKSWTSARDQ
                     VEGPTAALVEMRRGVLLDNLGRHDDAVEAFEDAMSHAPQWRDVYAGILSHLVSMPPDI
                     ELASSVWRRSQLHLTLPPEWKVYFTLWIQFITARAGEPPAEEHTELLRRLGASSNWWG
                     KLAKLGAGEIDYAALLSVASSLGEETEAIYYEGTRRFVAGDSEGAYEQFRRVLDTNMV
                     SFFEFEMARKLLLEEQKAGAPNP"
     gene            3299..>4661
                     /locus_tag="VFG22_17180"
     CDS             3299..>4661
                     /locus_tag="VFG22_17180"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HET6418031.1"
                     /translation="MIDFEKLGKLYLGRQFEPDRGEPNEVPVLYDSKDLTTHAVCVGM
                     TGSGKTGLCLTLLEEAAIDGIPALCIDPKGDLGNLMLTFPELRPSDFEPWIDPAEAER
                     KGRSVAAQAEAVSALWREGLAEWGQDGARIARFRDSVEVGIYTPGSAAGRPLRVLESF
                     DPPGVDADPDWQRDQIMGSVSSLLALLGLEPDPVNSKEHILLSNLLLHAWSRGESLEL
                     GDLVRGISDPPFARIGVMDLETFYPASERQKLAMRINGVLASPGFSAWLEGEPLDIQR
                     LLWTEEGKPRITILSIAHLSEPERMFFVSTLLNRVVSWVRSQSGTSSLRAILYMDEVF
                     GFLPPVAEPPSKTPLLTLLKQARAYGVGVVLATQNPVDLDYKALSNCGTWFLGRLQTE
                     RDVARVIDGLESASSAAGNALDRGAIERLLAGLRSRVFLLHNAHEEADVLFHTRWAMS
                     YLRG"
BASE COUNT          888 a         1442 c         1478 g          853 t
ORIGIN      
        1 gtgcaggtca catgggccga cgacgtcgag gtggatctgc cggtctcttt gcgcatcatg
       61 acggacgatc gggcggggat tctggcctcc gtatcgtcgg ttttctccgc aaatggggtg
      121 agcatcagtg aggcctcgtg cctgtctcgg gacggccgag ccgagaacat gttccatttc
      181 agggtcgggg acatcgaccg ccttcgagag atcatgcgcg gcgtggccaa gctcaaaggc
      241 gttctcgacg tcgagcgcgt ctaagcccgg ctggcatctt ccgtcgccct ccagaggatc
      301 gccaacaatg caacgagcgc gcctgacgag aggagcagca accagacgct cacgtgctcg
      361 acccgctgac tgagggtgtt gaacccttcg atgcgcagca agcttgccgg taagagcagc
      421 ggtagtgcgt tgactccggc gtgcatcgca atgctcgcca aggtggattt ggttcgcaga
      481 gccacggcgc cgagcaccag accgccgagg gtcgcgtaga ggatggccga aagctcgagg
      541 tgcaccaggc cgaagaggag tgagctccag agcagtgcct ttctttggcc gtattggtct
      601 ttgaggtctt gaagcaacca acctcgaaag aggagctcct cggtgagcgg cgccaccaag
      661 accagagcca acaagaccga cgatccgccc caccagctcg tcggattgac caatcgatgg
      721 cgtcgcgcga gctcttcgaa gctcaccggc cagacctctt gaaccaggtt gccgagctcc
      781 gcgagcggga tctgcatgag ggcgcccgct aggaatgaga gggcgacgat gcctccggcg
      841 agaggaagca cgttcacgga ttcgagaaga ccgtcctctc gcttcttgta tggaaacgca
      901 atgatgaaga tgagaccgac gctcgcagcc tgcaccagcg cgaggttcaa gggagcctga
      961 gcagccgccc gaatcgcttc gtgcgagctc gccgacaagc ttctgagctg aaggaaccct
     1021 gccagcccca agaagaggtg atttagggca gggaccgcta acgcgatcag gagtgctcgt
     1081 agatatcctc ccacgatgaa gcgtagcctg acacgtactc cagggttaca cgcaagatat
     1141 gagcctgccc tggtcggctg accctgctat agcggctctg caacatgcgg atccgtttcc
     1201 ttccaattct tggcttgctg actctcacgg tagcgtgcgc ccactcgaag tcggccacgg
     1261 tcgggtcccg cgtcgggagc gcagagccca tcccgcaagc ggagtacccg gatgaggccc
     1321 gccgtttcat gaggatgtcg ctcgacgatc ccgaacgggt cggcctgcga gatcgacttg
     1381 tagcctccgt tctttcgcag gctcagagcc ttgctgaggt ggacgactac gaaggcgtcg
     1441 tgtccgaggt cagcgagttg acgacctttc tctctccaca cgactacgcg aacggaatcc
     1501 ttcccaagag cgtcgagccg atcgcgcagt acatggccga agaaggcggt caccgaggtg
     1561 atgaagggaa cgtcctcgcg gcgaacttct tgctcgaacg cctgaccggg gacgaaattt
     1621 accagcagcg ctacgaaacc gtcgcaagct ggggccacga atcacgcgcc aatctggaca
     1681 ccgtctccga gcagtacgcc ggtttgatcg cggtctggaa tgagcacgcg gagctcacgc
     1741 cggcgcccaa ggtgctcgat acgctcgccg acctgtacat cgcgggacgc gacgcggttt
     1801 cgagggaaac cgtcgaaacc cgccatcggc tcggtatcgg aggcgaacgt caactgaggc
     1861 tcgcgccctt gaacgtcgcc gcggtctacc tttcacacgg ggacatcgag tccgccgtct
     1921 cgaaggtcgg gacgatgggg tctgggggcg agcttcagag ccgtctgctc gaccttctga
     1981 cgaaagctca gagcgccgac gaggccggtg ccgcggcgac cttcgagctg gtcgaaggct
     2041 accgcctggc ccgatccgat gtcgccgccg gactttgccg ctcggcgctg ctgaggttcc
     2101 ccaaagatga gcggtttccg acctgcatgg cccgtgtcgc cgccgattcg gagcaatttg
     2161 cggacgcgac cgcttggtac gtgattgcaa tcgagctcga tcccgagtcg atcgacctct
     2221 acgatgaagc gctcgggcag ctcgacgagt tcatcgagac gcggctttcc gacccacatc
     2281 ccgaacgatc gagcgccctg gcccacggcg ccgagacgat tctggaggaa cgccagaagc
     2341 gttggcccga ttccaagcct ccgatcgcgc cgaacgagct cgagtttttg atcggtatgc
     2401 tcgagatgaa cgccggccat gtcgtcgagg cccagcagcg attcgaaacg agcctggcca
     2461 aagacgagaa cgtgggagct ctgttgcagc tcggcttgtt gtttgagcgt accgggcggc
     2521 ctctcgaagc ggaacagcga taccgtcgcg ccctcgagct cgcgccctcc gatacgctag
     2581 cggaagacgt tcgccgcgcc gagatcctag aaaatctcgg aaacgcggct cgggcccagg
     2641 gcaagacccg cgagatgacc acgcactaca agagcgcact caagtcctgg acgagtgcgc
     2701 gtgaccaagt cgaagggccg accgccgcct tggtcgaaat gcgccgagga gtcttgctcg
     2761 acaatctggg gcgtcacgat gatgcagtcg aagcgtttga ggacgcgatg tcacacgcgc
     2821 cccaatggcg tgacgtctat gccggcattc tttcccactt ggtttcgatg ccgcccgaca
     2881 tcgagctcgc gtcctcggtc tggcgcaggt cacagttgca cctcacgctg ccgcccgaat
     2941 ggaaagtgta cttcacgctt tggattcaat tcattaccgc ccgcgcgggg gagcctcctg
     3001 ccgaggagca cacggagctg ctccgcaggc taggcgcgag ctccaattgg tggggtaagc
     3061 tcgccaagct cggcgccggc gagatcgact acgccgcgct gctgagcgta gcgtccagcc
     3121 tcggcgagga gacggaggcg atctactacg aaggcacacg gcgcttcgtc gctggcgact
     3181 ccgagggggc atacgagcag ttccggcgcg ttctcgacac caacatggtg agcttcttcg
     3241 agttcgaaat ggctcgcaag ctgctgctcg aggaacagaa ggccggggcg ccgaatccgt
     3301 gatcgacttc gagaagctcg gaaagctcta cttgggcagg cagttcgagc ctgatcgggg
     3361 cgagccgaac gaggttccgg ttctctacga ttcgaaggat ctgacgacgc acgcggtctg
     3421 cgtgggaatg acgggcagcg ggaagacagg cctctgcctc acactcctcg aagaagccgc
     3481 catcgacggc atccccgccc tttgcatcga tcccaaggga gatctcggga atctgatgct
     3541 cacctttccg gagcttcggc cgagtgactt cgagccttgg atcgatccgg ctgaagccga
     3601 gcgcaagggt cgatcggtcg cagcgcaggc ggaggcggtg tccgctcttt ggcgagaagg
     3661 tctcgccgaa tggggtcaag acggagcgcg aattgcgcgc ttcagggact cggtcgaggt
     3721 cgggatctat acgcctggca gtgctgcggg gcggccgctt cgggtcctcg agtcgttcga
     3781 cccgccaggc gtcgatgccg atccagattg gcagcgcgat cagatcatgg gttcggtgtc
     3841 gagtctcctc gcgctactgg gcctggagcc cgatccggtg aacagcaagg agcacatcct
     3901 cctctcgaat ttgctgctgc acgcctggtc caggggagag tcgctggagc tcggcgatct
     3961 cgtccgcggc atctcagacc cgccgttcgc tcgcatcggc gtcatggatt tggagacctt
     4021 ctaccctgca tccgagcgcc agaagctcgc gatgcggatc aatggcgtcc tggcgtcacc
     4081 gggcttctcg gcctggctcg agggcgagcc tctagatatc cagcgtttgc tttggacaga
     4141 ggaaggcaag ccacgaatca cgattctttc gattgcgcac ctgagcgagc ccgaacgcat
     4201 gttcttcgtg agcaccttgc tgaaccgtgt ggtgtcctgg gttcgctcgc agtccggcac
     4261 gtcgagcctg cgcgcgatcc tctacatgga cgaggtcttc gggttcctgc cacccgtagc
     4321 agagccgcct tcgaaaaccc cgctcctcac cttgctcaag caggcccgcg cgtatggagt
     4381 cggcgtcgtg cttgctacgc agaaccccgt cgacctcgat tacaaggcac tgtcgaactg
     4441 cggcacttgg ttcttagggc gccttcagac cgagcgtgac gtcgccaggg tgatcgacgg
     4501 cttggaaagc gcttcatcgg ccgccggcaa cgcgctcgat cggggcgcaa tcgaacgact
     4561 tctcgccggc ctgcgaagcc gagtgttcct gctccacaat gcccatgaag aagcggatgt
     4621 cttgttccac acgcgctggg cgatgtcgta cttgcgtggc c
//
LOCUS       DASRZF010000387         3538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_292343, whole genome shotgun sequence.
ACCESSION   DASRZF010000387 DASRZF010000000
VERSION     DASRZF010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292343"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1374)
                     /locus_tag="VFG22_17185"
     CDS             complement(<1..1374)
                     /locus_tag="VFG22_17185"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET6418032.1"
                     /translation="MSIHRYWAIVAITITLFGLGAVRVDAQARVDSGEVGTPTGESQT
                     LTEAPLADGDDAAAIALRSLYVPGGLTSREAVTQIVAVSPDLDRVRALLLEAKGGAME
                     AMSGVVPRLELIGRYSRLSPVDNPTFEIPGLGALTFPSLDQQIASDATLAYSFTGSLA
                     ESLPAYRAAKKNEVAAGFQVEAARNDVAFAGQQAYYAFARAKADLGVAEFALEAAESQ
                     RKETDALVQAGSAARVDLLRVEAQEEAARVAVERAKLALQVTTRSLQALMLTEELPTL
                     GEDLSQPVGGVPSEPEVALKERAFRERPDLHALRTYIEARKHQLRSAIGGKLPDLLVR
                     ASAQYSNPNLRVIPQEDRFVATWEAGAVIRWAPNDTVQAQGLSKRIQAELDRAHADVI
                     ALEQAIRVEVAQGYHGMHAAQSAMESARLGLEAAREGYRVTRDQLQAGIVNTTTLLQA
                     QSELIRAQ"
     gene            1460..2470
                     /locus_tag="VFG22_17190"
     CDS             1460..2470
                     /locus_tag="VFG22_17190"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF011456.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HET6418033.1"
                     /translation="MAQGLKVAVVGATGMVGTEMLRVLEQRAFPVSELVPVATARSAG
                     KKVTFNGVEHTVVEISPEVFEGVDLALFSAGGGTSREWSPIAASKGALVVDNSSAFRA
                     DPEVPLVVPEVNAAAAANRPKGIIANPNCSTIQMVVALKPLHDEGRLKRVVVATYQAI
                     SGSGALAVAAFEAQEQAMARGEQVDKQKLSGQLSRNLLMHWKPDPETGYQEEELKMVS
                     ETRKIFGDPDIAVSPTAVRVPVITAHSEAITVETHRPISAARARELLSQAEGVVVVDD
                     LSAGIYPQPIDAAGKDAVYVGRIRADVGNPGGIQMWVVSDNLRKGAALNAIQIAEKVL
                     LK"
     gene            2592..>3538
                     /locus_tag="VFG22_17195"
     CDS             2592..>3538
                     /locus_tag="VFG22_17195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418034.1"
                     /translation="MQRQILFLSTLVWALGAFAPNTYAQIVDRISSINGEDFQGNDQK
                     RIFNRSDCGLDPQGGTGGTGGTGGSALDAGVGGTGGGAATATATAPKGSPEETFFDIR
                     LGDSGSAVSSVFLWVGTGGAECEKLEERNGTQGNCAEMSGNPQQVGTNFLVSGLTLQN
                     LIDASSGFGDIVSCASSGLTGTPYKIFAFRNDPPSSDVPATNYGVTEFYVDVVSPAPP
                     LVNTTPQEASTFTISWANPNPPDDIQAWLADASDVDDPSTADPVGSPINLASRDVSFR
                     ADQLGLTEAGDTAYVFVQAYDQAFVSDSLGGNQSALSEGV"
BASE COUNT          647 a         1121 c         1139 g          631 t
ORIGIN      
        1 ctgcgcacga atcagctcgg actgcgcttg cagcaaagtg gtggtgttga cgatcccggc
       61 ttggagctgg tcccgcgtga cgcgatagcc ttcgcgcgcg gcttcgagcc ctagcctggc
      121 cgactccatc gccgactggg cggcgtgcat gccgtggtag ccctgggcca cctcgactcg
      181 gatcgcttgc tccagcgcga tgacatcggc gtgcgctcga tccagctccg cttgaatccg
      241 cttgctgagg ccctgcgcct ggacggtatc gttcggcgcc catcgaatga ccgcgccggc
      301 ttcccaggtc gccacgaatc gatcttcctg cggaatcact cgaagattcg gattcgagta
      361 ctgagcactg gctcgaacga gcaggtcggg aagcttgccc ccgatcgccg atcgcagttg
      421 atgtttgcgc gcttcgatgt aggtgcggag cgcgtgcagg tccggtcgtt cccgaaaggc
      481 gcgttccttg agcgcaacct ccggctcact cggaacgccg cccacaggtt gggagagatc
      541 ttccccgagt gtgggtaact cttcggtgag catgagagct tgaagtgagc gcgtcgtgac
      601 ttgcagcgca agcttcgcgc gttcgaccgc cactctggcc gcttcctcct gcgcttcgac
      661 gcggagaagg tcgacacggg cggccgagcc agcctgcacc agagcgtcgg tttctttccg
      721 ctgcgactcg gccgcttcga gcgcgaactc ggccacccca agatctgcct ttgcccgcgc
      781 gaacgcgtag taggcctgtt gccctgcgaa tgcgacgtcg ttgcgcgctg cttcgacctg
      841 aaagcccgct gcaacctcgt tcttcttcgc ggcacgataa gcgggaagcg attcggcaag
      901 cgaaccggtg aacgagtacg ccagcgtcgc gtcgctcgcg atttgttgat ccaaggacgg
      961 gaaggtcaaa gcgccgaggc caggaatctc gaaggtcggg ttgtccacag ggctcagccg
     1021 cgagtagcgg ccgatcagct ccagacgtgg aaccacgcct gacatggctt ccatggcgcc
     1081 gcctttggcc tcgagcagta gggcccggac tcggtcgaga tccggtgaga ccgccacaat
     1141 ttgagtcacc gcttcgcgcg aggtcagccc gcctggcacg tagagggaac ggagagcgat
     1201 cgcggcggcg tcatcaccgt ccgcgagcgg ggcctccgtc agcgtctggc tctcgcccgt
     1261 aggcgtcccg acttcgcccg aatcgacgcg cgcttgggca tcgacccgaa ccgcgcccag
     1321 gccgaaaaga gtgattgtga tcgcgacgat cgcccagtac cgatgaatgc tcatggatgc
     1381 tccttgccaa gggcatgata cgccagctcg ccgagggcca gcttgccggt tccacggcgc
     1441 tcctctatag ccgtcgccta tggctcaggg actcaaagtt gcggtggtgg gagccaccgg
     1501 aatggtgggc acggagatgc ttcgcgtgct cgagcagcgt gcgtttccgg tttcggagct
     1561 ggtgcccgtg gcgacagctc ggagcgccgg taagaaggtg accttcaacg gcgtcgagca
     1621 cactgtcgtc gagatctcac ccgaggtgtt cgaaggggtc gacttggcgc tcttctcggc
     1681 cggaggcgga acctcgcgcg aatggtcgcc tatcgcagca agcaagggcg cgctggtggt
     1741 cgacaactcg tcggcgttcc gagccgaccc cgaggtaccg ctggtcgtgc cggaggtgaa
     1801 cgccgccgct gcggccaacc ggcccaaggg catcatcgcc aacccgaact gctcgaccat
     1861 acaaatggtg gtcgcgctca agccgctcca cgacgagggg cggctcaagc gtgtcgtcgt
     1921 ggcgacctac caggcgatca gcggttcggg ggccttggcg gttgccgcgt tcgaggcgca
     1981 ggagcaagcc atggctcgcg gcgaacaggt cgataagcaa aagctttccg ggcagttgag
     2041 tcgcaatctt ctgatgcatt ggaagccgga cccggagacg ggctaccagg aagaagagct
     2101 caagatggtc tcggagactc gcaaaatctt cggcgaccct gacatcgctg tctctccgac
     2161 ggcggtccgt gttcccgtga tcaccgcgca cagcgaggcc atcacggtgg aaacgcaccg
     2221 gccgatcagc gctgcgcgcg cccgggagct gctcagccag gccgagggcg tagtggtcgt
     2281 cgatgacctg agcgccggga tctacccgca gccgatcgat gctgcaggca aggacgccgt
     2341 ctacgtgggc cgaatccgcg ccgacgtggg caatcccggc ggcattcaga tgtgggtggt
     2401 ctcggacaac cttcgcaaag gcgcggccct caacgcaatc caaattgccg aaaaagttct
     2461 attaaagtga cgaaatgtca gggcttggcg tagaagaagg tcggatcatg tcagattgtc
     2521 cgctgccccc tcgaaaacac acagctttcg ggcgccccac gttggcccgg ggattgcacg
     2581 cgaggaaggg catgcagagg caaatcttat tcttgtccac gctcgtctgg gcgctgggcg
     2641 ccttcgcgcc gaacacttac gctcagatcg tcgaccgtat cagctcgatc aatggcgagg
     2701 atttccaagg caacgatcag aagcgcatct tcaaccggtc ggattgcggg ctcgacccgc
     2761 agggtggcac gggcggcact ggaggtacgg gcggcagcgc tttggacgcc ggcgtaggcg
     2821 ggactggcgg cggcgcggcg actgcgactg cgactgcgcc taaggggtcg cccgaggaga
     2881 ccttcttcga catccgtcta ggggattccg gcagcgcggt atcgtccgtc tttctctggg
     2941 taggcaccgg gggcgcggaa tgcgaaaagc tcgaagagcg caacggcacg cagggcaact
     3001 gcgctgagat gtcgggtaat ccgcaacagg tgggaaccaa cttcctcgtg agcgggctga
     3061 ccttgcagaa tctgatcgac gccagctcag gcttcggcga tatcgtcagt tgtgccagct
     3121 ctggtctaac cggtacccct tacaagatct tcgcgtttcg caacgatcct ccctcgagcg
     3181 acgtcccggc caccaactac ggcgtgacgg agttctacgt agacgtcgtc tctcccgccc
     3241 cgccactggt caacacgacg ccacaagagg catcgacgtt cacgatctct tgggccaacc
     3301 cgaatccgcc ggacgacata caagcatggc tagccgacgc gtcggacgta gacgatccct
     3361 caacggcaga tcccgttggc agccccataa atctcgccag tcgagacgtg tcgttccgtg
     3421 cggaccaact cggcctcacc gaagcgggcg acacagccta cgtatttgtg caggcctacg
     3481 accaggcgtt cgtgtccgac tcgctcggcg gcaaccagag cgcactctcc gagggcgt
//
LOCUS       DASRZF010000388         8580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3730260, whole genome shotgun sequence.
ACCESSION   DASRZF010000388 DASRZF010000000
VERSION     DASRZF010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 8580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8580
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3730260"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1354)
                     /locus_tag="VFG22_17200"
     CDS             complement(<1..1354)
                     /locus_tag="VFG22_17200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418035.1"
                     /translation="MRSSCCSALLAFAVVGVLGTASSAHAWTLTVVNGARATIDVEPD
                     ATLSILLRLDVEVHAGWLHELELVDLGQGVELDRNRPPYVRSDDGEIFRPEFEVHEDG
                     RIHLTFPRREAPRRGEYRVFMRYRTKADVQAVELRDGPRARVVWSLPTWETGLHDVSV
                     EVRAPKGSSVPPELHDGAPGVDFQLSQHANRTVVAWRRTHLPRMTAWPLAVDVPVESI
                     AMPVTESDAAIPSGFRALPSPDERPIVWSLLLIAMLILVKRRSIEARLGERFLWVRAP
                     WGVVLAITAFVVAIGQWLGPNHLVCAIPLLAFALHRPASWSPTIQRKNWGPAPAKRLL
                     DNEMSAGDLLDGTTFVGIAALMAGAAALVVLAEPSAAILLLPLFFTGTRHHFHPTAAE
                     NTRMLYRFAADLRLPIDAPEMSFGWERATEDGALRLRAHLATQRAGLLTLGFVVATSP
                     V"
     gene            complement(1395..2315)
                     /locus_tag="VFG22_17205"
     CDS             complement(1395..2315)
                     /locus_tag="VFG22_17205"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET6418036.1"
                     /translation="MRRENTKWGLPLILIALTFASTFYVGAGMVLGAPPDSLEELRAG
                     WVFSVPLMAILLAHEFGHFFAGRYHHVDVSAPYFIPVPFFLLGTMGAVIQIREQIRSR
                     NALLDIGAAGPLAGMLVALPVLIYGIANSPVQPLPDTEYILEGRSLLYLGLLDLLKGP
                     IPEGYDIMLTPTALAGWAGLLVTMINIIPFGQLDGGHVAYALMGPRQDQHSRTVLRLL
                     PVLGVLVSAVYGISSYVEGIRGDALINDASAGMHWLVWALLLWALTRATGPGHPPTED
                     DTLSPRRRVVGWFTLSLFVLLFMPSWIRIQ"
     gene            2372..4267
                     /locus_tag="VFG22_17210"
     CDS             2372..4267
                     /locus_tag="VFG22_17210"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HET6418037.1"
                     /translation="MDLYSTAKRLNRPFVFRDEASKRALTVADHGLIGDGFSAALVRA
                     DGAIDWLCLPRFDSPSVFGALLDEGNGGSTSVSPVDYPFESLQRYDPGTNVLETVFKV
                     DGRGTLRLTDYMPWTDDPRSAMHEVHRRVQCVEGEVSVKVLFDPRFDYGRADTRVELD
                     GRSALATSATGERLIAVLGGTGQWENRPQGGVQAVFRLKAGERAWVVISWNTTESESV
                     LAYRPFDALRKTRRRWRDWAQQLDYDGPWRHHVIRSALLLKLLMYAPTGAMVAAPTTS
                     IPEWIGGVRNWDYRYTWTRDTAMAVRAANLLGCNREAREFFHFVRDTLERDRKLHIMY
                     AVDGGRVPDERTLDHFSGYRGSLPVRVGNGARDQLQLDTAGALMDAAYVYERGGGSLT
                     ARGWRRLREVVENVAATWGEPDHGIWEPRAGKRHNVHSKLMSWLALRRGAELAPLFGE
                     QPLRTRWNRVADQLHTELCARGLDRTGKRFVAAYDHEHPDASLLLLPIHGFLPPNDQR
                     VLRTVDWIQAELGDGPYVYRYRGDDGVGGEEGAFVLCGFWLAEVLAMAGRIDEAQQVF
                     VAHAEASNHLGLLSEEIEPGTKNLLGNFPQAFSHLGLINAAARIDLALRLRDEGSPRV
                     PEVEIGR"
     gene            4279..5259
                     /locus_tag="VFG22_17215"
     CDS             4279..5259
                     /locus_tag="VFG22_17215"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET6418038.1"
                     /translation="MSSADGVDVRGFVTARVGAYAALVALLTAAGLWFATGIRGVDVW
                     GSFAIASLVGIGGAVWGVRSALASVSGTSVVQPSEISRAESEAVESLTPSSEDTNDEP
                     QAEPDEVDYLMALRHEFRTPLNAVLGFSDVLLSGIDGEVNASQREDLEIIRASGIRLR
                     ILLDSALDLSQLTSGELRVSAERTDIRELVARVAVEAGQLWSNKRRAGCTLPDEPCVT
                     DADEARLRRSILVLADFLATDHRDAEIRLSLSRSAHYLAIEIAADPSDRLNIVALPTP
                     AEVLAAEDAMEIRRWPVAVTSEVIARHDGSLYHGDHPSRFMIRIPLRGAP"
     gene            5256..6224
                     /locus_tag="VFG22_17220"
     CDS             5256..6224
                     /locus_tag="VFG22_17220"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HET6418039.1"
                     /translation="MTNAGDRVVSNLRWTVIAISSYFTLCWMLIAGHDGYWGAPVLFG
                     LALTLGWGLLRRLIPSLRFTEPSVAPPAIHPSAPTSMEETIEAHSAHPELPERQRARF
                     LGALGHELRNPLNSVTGFSDMLLGRIDGELNAEQEQSVRTIRAGAERLLRLVSTVVDR
                     AKVEAGTLRLQQQWVAPDELISEAIGFVRSRAEDTSVVGGVSEGLPRVFVDKHRTVHA
                     LVSLAVLVIRSSETDIVMRVAPPRRDQVGKGFVQFDLMATLKDSSADTLTIVERLEKP
                     LSRSDALGLEVAVARSVIEAQGGRIWTEAGTAGSLRVCMTLPTSAH"
     gene            complement(6221..7060)
                     /gene="rsmI"
                     /locus_tag="VFG22_17225"
     CDS             complement(6221..7060)
                     /gene="rsmI"
                     /locus_tag="VFG22_17225"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HET6418040.1"
                     /translation="MPGRLSVVATPIGCLEDITLRALRVLREADLILAEDTRHTRTLC
                     SKHGIDTPLRSFHAHSKQEKVDIIVAELQAGAHYAMVSDAGTPVVSDPGVYLVSCAAR
                     AGIEVEAIPGPSAVLAALSVAGLPVRGFVFEGFLPRGGRDRTRVIERIARSELTVVLF
                     ESPHRIHSTLGELGSQLGTDRQVALCRELTKMHEQVIRGTIEEVERELSKPARGEITL
                     VITGKESSAPVADVDVEGLISEWTLEGLSTKEMSQRLQDDLGWKRNVAYQAVLDAQKS
                     EQS"
     gene            complement(7104..8090)
                     /locus_tag="VFG22_17230"
     CDS             complement(7104..8090)
                     /locus_tag="VFG22_17230"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF005143.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HET6418041.1"
                     /translation="MIGNDEETPDQIDEPEDVELMDEEPLEGGPEPRALAASASALER
                     RDPLGVYMRDVQRYPLLSKAEEHDLAVRYFENDDVDAAKLLVTSNLRLVVKIAYDYRQ
                     AYKNILDLVQEGNIGLMQAVKKYDPYKGVKLSSYAAWWIRAYILRYILNNHRLVKVGT
                     TQAQRKLFFNLQKEKAKLSAMGIEPTSQIIAERLNVPERDVISMDMRLASGDASLDAP
                     VGTADGRSVARVEILPSGAARADDTLADAEVGDRFSAKIHEFGNTLKGKEKKIFEDRL
                     VSEDPKTLQTLGDDFGVSRERVRQIEKRLLQKLKAYLRDELGETVVEVYEAT"
     gene            complement(8152..>8580)
                     /gene="gspN"
                     /locus_tag="VFG22_17235"
     CDS             complement(8152..>8580)
                     /gene="gspN"
                     /locus_tag="VFG22_17235"
                     /inference="COORDINATES: protein motif:HMM:TIGR04411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspN"
                     /protein_id="HET6418042.1"
                     /translation="ELTIEGLNVGDGESKIEIPGWGGLTLDRADVGDLTFIAGIEDGV
                     AEIERAEAHGKDLEFDALGRLGLSRPLKRSELNVMVRVKIQDAYKERSSKVATMLELA
                     SSGLEAAQSADGAIQYMVAGPIGGRMRPRAAGRLPFEAPK"
BASE COUNT         1599 a         2590 c         2704 g         1687 t
ORIGIN      
        1 caactgggct ggtcgcgacc acaaagccca aggtcaagag cccggcgcgc tgcgtcgcga
       61 ggtgagcgcg gagtcggagc gccccgtcct ccgttgcgcg ctcccaaccg aatgacatct
      121 ccggcgcgtc gattggaaga cgaagatcgg ccgcgaaccg atacagcatc cgagtgtttt
      181 cagcggccgt cgggtgaaag tggtgacgcg tcccggtgaa gaagagtggc aacaagagga
      241 tggccgcact cggctcagcc agaaccacga gagcagcagc ccccgccatc agcgctgcga
      301 tccccacgaa agtcgttccg tctagcaggt cgcctgccga catctcgttg tctaagagcc
      361 tctttgctgg tgccggaccc cagttctttc gctgaatcgt tggcgaccag cttgctggtc
      421 gatgtagagc gaacgcgagt agcgggatgg cacagacgag atggttcggg ccaagccatt
      481 gaccgatggc gacgacgaac gccgtgatcg ccaggaccac cccccagggc gcgcgaaccc
      541 acaggaagcg ctcgcccaat cgcgcctcga tggaccttcg cttgacgagg atcagcattg
      601 cgatcagcag cagcgaccag acgatcgggc gctcgtcggg actcggcagg gctcgaaacc
      661 ccgaggggat ggcggcatcc gactcggtca ccggcattgc aatcgattcg accggaacgt
      721 cgaccgcaag cggccatgcg gtcattcgcg gcaagtgagt tcgcctccat gcgacgacgg
      781 ttcgattcgc atgctgggag agctgaaagt ccacgcctgg ggcgccgtcg tgaagctcgg
      841 gaggaaccga ggatcccttc ggggcgcgga cctcgaccga tacgtcgtgg agtccggttt
      901 cccaggtagg cagtgaccaa acgacgcggg cgcgcggtcc gtctctcaac tcgacggctt
      961 ggacgtccgc tttcgtccgg tatcgcatga acacccggta ctcgcctcgt ctcggagctt
     1021 cgcgtctggg gaatgtgagg tgaattcggc cgtcttcgtg aacttcgaat tcgggacgga
     1081 agatctcgcc atcgtcggaa cggacgtacg gtggtcgatt gcggtcgagc tccacgcctt
     1141 gaccgaggtc tacgagctcg agctcgtgaa gccagccggc atggacctcg acgtccaggc
     1201 gtaggaggat gctcaaggtg gcgtcgggct cgacatcgat tgttgcgcgc gccccgttga
     1261 cgaccgtgag ggtccaggca tgggctgacg aggcggtacc gagcacgccg acgacggcga
     1321 aagcgaggag ggctgagcag cagctggagc gcatgccccg aagctaagga attccagcgt
     1381 ttcagtgaaa tttcctactg gattcggatc caggagggca tgaacagcag aacgaacagc
     1441 gaaagcgtga accagcccac gactcggcgc cgaggtgaga gcgtgtcatc ctcggtcgga
     1501 ggatggccgg gaccggtcgc gcgggtgagc gcccagagaa gcaatgccca caccagccag
     1561 tgcatccccg cggacgcgtc attgattagc gcgtcgcccc gaatgccctc gacgtagctc
     1621 gaaatcccat acactgcgct cacgagaact cccaatacgg gaagcagtcg gagcacggtt
     1681 cgcgagtgct ggtcttggcg cgggcccatg agcgcgtacg ctacgtggcc gccgtcgagt
     1741 tggccaaacg ggatgatgtt gatcatggtg acgagcaatc cggcccaacc ggcgagagcc
     1801 gtcggtgtga gcatgatgtc gtaaccctcc gggatcggtc ccttcaagag atcgagcagg
     1861 ccgagataga gaagggagcg gccctccaaa atgtactcgg tatccggaag aggctgcacc
     1921 ggggaattag cgatgccgta gatcagtacg ggaagcgcga ccagcattcc ggccagaggg
     1981 cctgctgcgc cgatgtcgag cagagcgttt cgggagcgga tttgctcccg gatctggatc
     2041 acggctccca tggtaccgag gagaaaaaag ggcactggga tgaagtacgg cgctgaaacg
     2101 tcgacatgat gatagcggcc ggcgaagaag tgtccgaact cgtgtgcgag taggattgcc
     2161 atcaggggga cggaaaagac ccagcccgcg cggagctcct cgagcgaatc gggcggagct
     2221 cctaggacca ttccggcacc cacgtagaag gtggacgcaa aggtcagagc aatgaggatg
     2281 agtggcagcc cccacttggt gttctcgcgc cgcaccgcgt gcctctagca tgtgcgatac
     2341 gaagcgcacg atctgctatt cttcgaagcc gatggatctc tacagcaccg cgaagcgact
     2401 caatcggccg tttgtcttcc gcgacgaagc ctcgaagcgg gcgctcaccg tcgcggacca
     2461 tggattgata ggtgatgggt tctccgccgc gctggttcga gcggacggtg cgatcgattg
     2521 gctttgtctc ccacgcttcg acagccctag tgtttttggc gccttgctcg atgaaggaaa
     2581 cggcggttct acctcggtga gcccggtcga ctatccattc gagagtcttc agcgatacga
     2641 tcccggcacc aacgttctcg agaccgtgtt taaggttgac ggtcggggga cgcttcggct
     2701 caccgactac atgccctgga ccgacgaccc tcgctccgcg atgcacgagg tacaccggcg
     2761 agtgcagtgc gtcgagggag aagtgagcgt caaggtcctc ttcgatcccc gtttcgacta
     2821 tgggcgtgcc gacacccggg tcgaacttga cggacgctcg gcgctcgcca cgagtgccac
     2881 aggcgagcgt ttgatcgccg tgctcggggg cactggccaa tgggagaacc gcccgcaagg
     2941 tggagtgcaa gccgtcttcc gcttgaaagc aggcgaacgc gcatgggtgg tcatcagttg
     3001 gaacaccacg gagtcggagt cggttctcgc atatcggccg ttcgacgctc tgcgcaagac
     3061 acgccggcga tggcgcgatt gggcgcagca gctcgactac gacggacctt ggagacatca
     3121 cgtcattcgc tcggcgttgc tcctgaaatt gctgatgtat gcgccgaccg gcgccatggt
     3181 cgccgccccg accacatcga ttccagagtg gatcgggggt gtacggaact gggactatcg
     3241 ctacacctgg acccgtgaca cggcgatggc ggtacgggct gcgaacctgc ttggatgcaa
     3301 ccgcgaggcc cgcgagttct tccacttcgt aagggacacg ctcgaacgcg accggaagct
     3361 tcacatcatg tacgcggtcg acggcggtcg ggttcctgac gagcggacgc tcgatcattt
     3421 ttccggatat cgtggctcac tgccggttcg tgttggaaac ggcgcacgtg atcagctgca
     3481 gctcgacacg gcaggtgcgc tcatggacgc tgcctacgta tacgagcgcg gcggtggatc
     3541 gctcaccgca cgtggttggc ggcgactccg agaggtggtc gagaatgtcg cagcgacctg
     3601 gggcgagccg gaccacggca tctgggagcc gcgcgctggc aagcggcaca acgtgcattc
     3661 gaagttgatg agctggctcg ccttgcgtcg cggcgcggag ctcgctccct tgtttggtga
     3721 acagcctctg cgaacccgat ggaaccgagt agccgaccag ctacatacag agctttgtgc
     3781 tcgcgggctg gatcgaacgg gaaagcgatt cgtcgccgca tacgatcacg agcacccgga
     3841 cgcgtccctg ctcttgctcc caatacatgg ctttcttccg ccaaacgacc aacgcgtgct
     3901 acgaacggtc gactggattc aggcggaatt gggggatggc ccttacgtct atcgctacag
     3961 gggcgacgat ggtgtcggag gcgaggaggg cgccttcgtc ctctgtggct tctggttggc
     4021 cgaggttctt gcgatggcag gccggatcga cgaggcacag caggtgttcg tcgcgcacgc
     4081 cgaagcgtcg aaccacctgg gccttctctc cgaggagata gaacccggga ccaagaacct
     4141 gctcggcaat ttcccacaag ctttcagtca tcttggactg atcaatgctg ccgccaggat
     4201 cgacttggcg ctgcgtctgc gggatgaagg ctcgccgcgt gttcccgagg tcgagatagg
     4261 gcgatagtcg gggtgggcat gtctagcgca gacggggtgg acgttcgcgg gttcgtgacg
     4321 gcgagagtcg gcgcgtacgc tgcgctcgtt gcgctcttga ccgccgccgg tctctggttc
     4381 gcgaccggaa tccgcggagt ggatgtttgg ggctcgttcg caatcgcttc cctcgtcggg
     4441 ataggcggcg cggtttgggg tgttcgttca gcgctagctt ccgtttcggg cacatccgtc
     4501 gtgcagccga gcgagatcag tcgcgccgaa tccgaagccg tcgagagctt gaccccgtca
     4561 tcggaagaca ccaacgatga gccgcaagcc gagcccgacg aggtcgacta cttgatggcg
     4621 ctccgtcacg aattccggac accgctgaac gcggttctcg gattttcgga tgtcttgctg
     4681 agcggaattg acggagaggt caacgcctca caacgagagg acctggagat cattcgggcg
     4741 tcgggaatac ggcttcgaat cctcttggac agcgctctcg atctctccca gctcacatca
     4801 ggcgagctac gcgtgagcgc cgagcgtacc gatattcgcg agctggtggc tcgtgttgcc
     4861 gtagaagcgg gccagctctg gtccaacaag agaagggcgg ggtgcacgct ccccgacgag
     4921 ccctgtgtca ccgatgcgga cgaagcgcga cttcgccgtt ccatcctcgt gcttgctgac
     4981 ttcttggcaa ccgaccacag ggacgcggag atcaggctca gcctatctcg ctctgctcat
     5041 taccttgcaa tcgagatcgc ggccgacccg tcggatcgac tgaacatcgt cgcgctccct
     5101 acgccagcgg aggttctagc cgccgaggat gccatggaaa tccgcagatg gcctgttgca
     5161 gtgacctccg aggtgatcgc gcgccacgat ggctctctct accatggcga tcacccttcg
     5221 cgattcatga tacggattcc attgcgaggc gcgccatgac gaatgccggc gatcgagtgg
     5281 tttcgaatct tcgctggacg gtcatcgcaa tctccagcta cttcacgctt tgttggatgc
     5341 tgatagcggg gcatgacggc tactggggtg cgcccgtctt gttcggcctc gcactgacgc
     5401 ttgggtgggg tctgttgcga cgactgattc cgagcttgag gttcacggag ccatcggttg
     5461 caccgcccgc gatccacccg tccgccccca catcgatgga ggaaacgatc gaggcacatt
     5521 cggcgcatcc tgagctcccg gagcgtcagc gcgcgcggtt cctcggtgcc ctggggcacg
     5581 agcttcggaa ccctctcaac tcggtcaccg gcttctcgga catgttgctc ggtcgcatcg
     5641 atggggagct caatgccgag caggagcaga gcgtacgaac gattcgtgcg ggagcggagc
     5701 gactgcttcg tctcgtttcg acggtcgtcg accgcgcgaa agtggaggcc gggacgcttc
     5761 gactgcagca gcagtgggtt gcgcccgacg agctgatttc ggaggcgatt gggttcgtgc
     5821 gctcacgggc tgaggacacc tcggtcgtcg gaggagtctc cgaaggcttg ccacgtgtgt
     5881 tcgtcgacaa gcatcgcacc gtgcacgcgc tggtcagtct cgccgtgctc gtgattcgct
     5941 catccgaaac ggatatcgtc atgcgcgtcg cacctccccg tcgtgatcag gtcggcaagg
     6001 gcttcgttca gttcgatctc atggcaacgc tgaaggattc atcagccgac acgctgacca
     6061 tcgtcgaaag gctcgagaaa cctctttcac gatcggatgc gcttgggttg gaagtcgccg
     6121 tcgccagaag cgtgatcgag gcgcaaggcg gacggatttg gacggaggcg ggaaccgcgg
     6181 gaagtctacg agtttgcatg acgctaccga cttctgcgca ttaggactgc tcggacttct
     6241 gcgcatcgag gaccgcttgg tatgccacgt tgcgtttcca tcccagatcg tcctggagtc
     6301 gctggctcat ttctttggtg gacagtccct cgagcgtcca ttcagaaatc agcccctcca
     6361 cgtcgacatc tgcaacgggc gcgctgcttt cctttcccgt gatgaccagc gtgatctctc
     6421 cgcgcgccgg cttggatagc tcgcgctcga cttcctcgat ggtcccgcga atcacttgtt
     6481 cgtgcatctt ggtgagctct cggcaaagag cgacctgcct gtccgtgcca agctggctcc
     6541 caagctctcc gagtgtgctg tggatgcgat gcggcgattc gaagagtacg acggtgagct
     6601 cggaacgtgc gatccgctcg atcacgcggg tgcgatctct gccacctcgg gggagaaagc
     6661 cttcgaagac gaacccacga accggcaggc cagccacgct gagcgcagct aagaccgcgc
     6721 tcggccctgg aatcgcctcc acctcgatgc ctgcgcgagc tgcgcaggaa acgaggtaga
     6781 cgcccggatc gctcaccacg ggcgtcccag catcgctcac catcgcatag tgtgcgccag
     6841 cttgcagctc ggcgacgatg atatcgacct tctcctgctt gctgtgcgcg tggaacgacc
     6901 gcagcggtgt gtcgattccg tgcttcgaac agagtgttcg cgtgtgcctc gtatcctccg
     6961 ccaagatcaa gtctgcttca cgaagaacac gcagtgccct gagcgtgatg tcttctagac
     7021 atccgatggg cgtcgcgacc accgaaaggc gacctggcat ctaccggagt taccacatct
     7081 ggatacggcg gcgacccgag cgtctaagtt gcttcgtaga cttcgactac ggtctcgccg
     7141 agctcatcgc ggagataagc cttcagtttc tgaaggagac gcttttcgat ctggcgcacg
     7201 cgctcgcggc tcactccgaa atcgtctccc agcgtttgca gtgtctttgg atcctcggat
     7261 accaagcggt cctcgaagat cttcttttcc ttgcccttca gggtgtttcc aaactcgtgg
     7321 atcttggcag aaaaacggtc gccaacttcg gcatcagcca gggtgtcatc tgcgcgggcg
     7381 gcgcccgaag gtaggatctc cacgcgagcg acggaacggc cgtccgcggt tccgacgggc
     7441 gcatcgagag aagcatcacc ggaggccaaa cgcatgtcca tcgagatgac gtctctctct
     7501 ggaacattga ggcgttcagc gatgatctgc gacgtcggtt cgatgcccat cgcggagagc
     7561 ttcgccttct ctttttggag gttgaagaag agctttcgct gcgcctgtgt agttccgacc
     7621 ttgacgagac gatggttgtt cagaatgtac cggaggatgt aggcgcggat ccaccaagcc
     7681 gcatagctcg aaagcttcac tcccttgtac ggatcatact tcttcacggc ctgcatcagg
     7741 ccgatgtttc cttcttgaac caggtctagg atgttcttgt acgcttgccg gtagtcgtac
     7801 gcgatcttca cgaccaagcg gagattggac gtcaccaaaa gtttcgcggc gtcgacgtcg
     7861 tcgttctcga agtaccgtac cgcgagatcg tgttcctccg ccttcgatag taggggatag
     7921 cgttgaacat ctctcatgta gacgccgagg gggtcgcgac gttcgagcgc cgaggccgat
     7981 gcagccaacg cacggggttc agggccgccc tcgagcggct cttcatccat cagctcgacg
     8041 tcttcgggtt cgtcgatctg gtctggtgtc tcttcgtcgt ttccgatcat cgattcggct
     8101 cgcgatcacc ttctctaaag cacagccgcc ctctgcgtcc agtgggggcc ctcacttcgg
     8161 cgcttcgaag ggcagtcttc ctgcagctct ggggcgcatt cgacccccga tgggccctgc
     8221 aaccatgtat tgaatggctc cgtctgccga ctgcgcggct tcgagcccag atgatgccag
     8281 ttcgagcatg gtcgcaactt tcgagctccg ttccttgtag gcatcttgaa tcttcacacg
     8341 gaccatgacg ttgagctcgg agcgcttgag cggtcgcgaa aggcccagtc ggcccaaggc
     8401 atcgaactcc agatctttcc cgtgagcttc tgcgcgctca atctccgcta cgccatcttc
     8461 gatccccgcg atgaacgtca ggtcaccgac gtcggcgcgg tcgagcgtca gtccacccca
     8521 tccggggatc tcgatcttcg attctccgtc gcccacgttc agtccctcga tcgtgagctc
//
LOCUS       DASRZF010000389         2101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3049073, whole genome shotgun sequence.
ACCESSION   DASRZF010000389 DASRZF010000000
VERSION     DASRZF010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3049073"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(287..1642)
                     /locus_tag="VFG22_17240"
     CDS             complement(287..1642)
                     /locus_tag="VFG22_17240"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET6418043.1"
                     /translation="MKRFNRRTLLRGSLAGGAVTIGLPLLDLFLNEHGTALAQGAPLP
                     LRFGAWMWGCGMNPQRWTPTAEGTDYELPIELQALDRELPSGGRLRDVVSILSGFDVK
                     LDGRPNFPHTAGLMGTLTGSLPLQDYTVPAPTLDTIIAGAVGGLTRFRSLEMSCTGNP
                     NQSYSYESQSVFNAPEISPMAMYTRVFGPEFSDPNAGPFVPDARVMLRQSVLSAVKED
                     RDRLLQRVGTHDKERLDQYFTSVRQLEQQLDILLAGPPNLESCAKAPAPAAESLGTEL
                     GQSMSTNRLMAELLSFALACDQTRVFSMMFSDRISGLRQPGSSSLHHQLTHDEALDPA
                     IGYQPEATEFVFASMEAWADFVERLAAVPEGDGTLLDNCLVFGHSDVSLAKVHGITGV
                     PMMIAGRASGALKTGVHVAGATSPSTRVGLTVQQVMGLNTESWGTRSMEAVQPVSEIL
                     A"
     gene            complement(1639..>2101)
                     /locus_tag="VFG22_17245"
     CDS             complement(1639..>2101)
                     /locus_tag="VFG22_17245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1588 domain-containing protein"
                     /protein_id="HET6418044.1"
                     /translation="SIVENTTGELRTARERLEKHVTNDSCAGCHSLMDPVGLALENFD
                     AVGMLRTEENGASIDPSGELDGVQYDDAPGMGRALRDHPELGPCLVRSLYRYAVGRAP
                     EAGEELLLEYLSERFAASGFRVTELMRELVLSDGFRTSSGSRSASESGGPT"
BASE COUNT          396 a          663 c          644 g          398 t
ORIGIN      
        1 gggacgtctt cacggacctc ttttccagag ctagtaagcg agactccgtt ctgttcgacc
       61 gagaaagtgc ctggtaggtc ctgcgcgctt gcgccggctg tcaccgtcaa aattgttccg
      121 agcacgaaag caaagcgttt catcggttcc ccctaaccgt tgattcgacg tgctccttca
      181 ggagtcacat cccccgtcga tcagtgtcga agcgctgatc caaagcgtca atgagaaggg
      241 ccgaacactg tgggatggat cacagttccc gtacagagga gtctgcttag gcgaggatct
      301 cgctgacggg ctgtacggct tccatcgagc gggtgcccca actctcggtg ttcaggccca
      361 tcacttgttg gacagtgagt cctacgcggg tgcttgggga ggtcgcgccg gcgacgtgaa
      421 ccccggtctt caaagccccg ctcgctctgc cggcaatcat catcggcacg cctgtgatcc
      481 cgtggacctt cgccagcgag acgtccgagt ggccaaacac gaggcagttg tcgagcagtg
      541 tgccatcgcc ttccgggacc gcggccaggc gttctacgaa atcagcccag gcttccatcg
      601 aggcgaacac gaattcggtc gcctcgggtt ggtatccgat cgccgggtcc agggcctcgt
      661 cgtgagtgag ctggtggtgc agcgagctcg atcccggctg ccgcaggccg gaaattcggt
      721 ccgaaaacat catgctgaag acacgggtct gatcgcatgc gagtgcgaag gatagaagct
      781 ccgccatgag tcgattggtc gacatcgact gaccgagctc ggtcccaaga gactcggcgg
      841 caggggccgg cgccttcgcg cagctttcga gattcggcgg gccggctagc aaaatgtcga
      901 gctgttgttc gagctggcgc acggaggtga aatactggtc gagcctctcc ttgtcgtgcg
      961 tacccactcg ctgcagcagg cgatcacgat cctctttgac cgcggagagg acgctttgac
     1021 gaagcatcac gcgtgcatcc ggcacgaatg gacccgcatt gggatccgaa aactccggtc
     1081 cgaacacgcg tgtatacatg gccatcggcg agatctcggg cgcgttgaac acgctttgac
     1141 tctcgtagct gtagctttga ttcggattgc cagtgcacga catttcgagt gaccggaatc
     1201 gggtcagacc gcccaccgct ccagcgatga tggtgtccag cgtgggagcc gggaccgtat
     1261 agtcctgcaa gggaaggcta cccgtcagag tccccatcaa acccgccgtg tggggaaaat
     1321 tcggccgccc gtcgagcttc acgtcgaagc cgctgagaat gctcaccacg tcccgcaacc
     1381 tgcctccgga gggtaactcg cggtctaggg cctgaagctc gatgggaagc tcgtagtcgg
     1441 tgccttccgc agtcggtgtc cagcgctgtg ggttcatgcc gcatccccac atccacgccc
     1501 caaaccgcag tggcaatggc gcgccctgcg ccagcgccgt tccgtgctcg ttgagaaaca
     1561 gatcgaggag aggaagaccg atcgtcaccg cgccaccggc aagcgagcct cggagcagcg
     1621 ttctacggtt gaaccgtttc atgtcgggcc tcctgactcg ctggccgagc gtgacccgga
     1681 gctggttcgg aacccgtcgc tcaacacgag ctcccgcatc agctcggtca cgcggaagcc
     1741 cgacgcagcg aatcgctcgc tcaggtactc gagcagcaac tcttcgcctg cttctggggc
     1801 gcggccgacc gcgtagcgat agaggctccg caccaggcat ggaccgagct cggggtggtc
     1861 cctcaacgcg cggcccattc caggagcgtc gtcgtactgc accccatcga gctcacccga
     1921 gggatcgatc gaggcaccgt tctcttcggt gcgcaacatg cccaccgcgt cgaagttctc
     1981 gagggcgaga cctacagggt ccatcaagct gtgacagcct gcgcaagagt cgttggtgac
     2041 gtgcttctcc aagcgctcac gcgccgtccg aagctccccc gtggtgttct ccacgatcga
     2101 g
//
LOCUS       DASRZF010000390         2065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3732651, whole genome shotgun sequence.
ACCESSION   DASRZF010000390 DASRZF010000000
VERSION     DASRZF010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3732651"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1956)
                     /locus_tag="VFG22_17250"
     CDS             complement(<1..1956)
                     /locus_tag="VFG22_17250"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6418045.1"
                     /translation="MSKELRFDGRVAIVTGAGQGLGRSHALALAARGAKVVVNDLGGS
                     TSGEGKSAETADLVVEEIKKAGGEAVANYDSVEDGDSIVQTAMDSWGRVDIVVNNAGI
                     LRDKSFKNMTDADWDIIFRVHNFGAYKVTKAAWPIMTEQGYGRVLFTTSSAGLYGNFG
                     QTNYGSAKLSLVGFANTLSLEGQRKNVLVNTIAPFAASRLTEGLLPPAVFDSLKPEYV
                     TPIVAYLCSEANETTGGVFEVGGGFYSSLRWERTKGKLFRLGREVLPEAIASNWNQIN
                     DFSDAEHISSVMESLGPIIRNVEAGPSKGGNEFIDVDEALGFRYPDRTSSYDERDLAI
                     YALGVGAAIDPTDEGGLRLVYEGHGSGMKALPTFSVIPGTTAILGFAKEGITAPGLKY
                     GLDRVLHGEQYIELTRPLPLKATLTTKAIVKDIWDKGKGALVVTALDSYDEDGDLLIK
                     NEMTAFIRGAGGWGGDRGPSAEINLPPSREPDLIVEDSIPQNQALLYRLSGDWNPLHA
                     DPGMAKAFGFERPILHGLCTFGYAGRRVLESFAPDGNPDFFKSIQVRFASNVYPGNTL
                     ITEMWKESDQRIVFQCKVKERDGLVISNAAIELFEQLPKPKEKKMPAPAAEAPAASAE
                     PIAVDVIAAIDHFLKENPTIAEKAQTVF"
BASE COUNT          372 a          643 c          614 g          436 t
ORIGIN      
        1 gaacaccgtc tgcgccttct ccgcgatggt cgggttttct ttcaagaagt gatcgatggc
       61 ggcaatgacg tcgacggcga tcggctctgc ggaagcggcc ggtgcttcgg ctgctggcgc
      121 cggcatcttc ttctcttttg gctttggcag ctgttcgaag agctcgatgg ccgcgttgga
      181 gatcaccagg ccatctcgtt ccttgacctt gcattgaaac acgatgcgct ggtcggattc
      241 cttccacatc tccgttatga gcgtgttgcc agggtacacg ttgctggcga agcgcacctg
      301 gatgctcttg aagaagtccg gattgccatc gggggcgaag ctctcgagga cccgccggcc
      361 cgcgtagcca aacgtgcata gcccgtggag aatgggacgc tcgaacccga aggctttcgc
      421 catcccggga tccgcatgaa gcggattcca gtcaccgctg agacgataaa gcaatgcttg
      481 gttctgcgga atcgagtcct cgacgatcag gtcgggctcg cgcgacggag gcaggttgat
      541 ctccgcgctc gggccgcggt cgccacccca gccaccggca ccacggatga acgcggtcat
      601 ctcgttcttg atcaatagat ccccgtcctc gtcgtatgaa tcgagggcag tcacgaccag
      661 cgcgcccttc cctttgtccc agatgtcttt gacgatcgcc ttggtggtga gcgtcgcctt
      721 caaggggagc ggacgagtga gctcgatgta ctgctcgcca tgaagaacgc ggtctagccc
      781 gtacttcagg ccgggcgccg tgattccctc cttcgcgaag cctagaatcg cggtggtccc
      841 agggatcacg gaaaaggtcg gcaaggcctt catgccgctc ccatgcccct cgtagacgag
      901 ccgaagccct ccctcgtccg ttggatcgat cgcggcgccg acaccgagcg catagatcgc
      961 caagtcccgt tcatcgtagc tcgatgtacg gtcgggatac ctgaagccaa gtgcctcgtc
     1021 gacgtcgatg aattcgttgc cacccttgct ggggcccgct tcgacgttcc gaatgatcgg
     1081 gccgagtgac tccatcacgc tgctgatgtg ctcggcatcg ctgaagtcgt tgatctgatt
     1141 ccaattcgac gcaatggcct cgggtaacac ttctcgaccc aggcgaaaca gctttccttt
     1201 ggtgcgctcc catcgaaggc tcgaatagaa tccgccgccc acctcgaaga cgccgccggt
     1261 ggtttcgttc gcctcactac aaaggtaggc gacgatcgga gtgacgtact cgggcttcag
     1321 cgagtcgaag accgccggag ggagcagccc ttcggtgaga cgagacgccg cgaacggcgc
     1381 gatcgtattc acgaggacgt tcttgcgttg gccttcgagc gacaatgtgt tcgcgaagcc
     1441 gacgagactc agctttgccg atccgtaatt ggtttggccg aagtttccgt aaaggcccgc
     1501 ggacgaggtc gtgaagagca cacggccgta gccctgctcc gtcatgatcg gccaggcagc
     1561 cttggtgact ttatacgcac cgaagttgtg gacgcggaag atgatgtccc agtcggcgtc
     1621 ggtcatgttc ttgaacgact tgtcgcgtag gatgccagcg ttgttgacga cgatgtcgac
     1681 gcgcccccag ctgtccatcg ccgtctgcac gatcgagtca ccgtcttcga ccgagtcgta
     1741 gttcgcgacc gcctcgccac cggccttctt gatctcttcg acgaccagat cggcggtttc
     1801 cgccgacttg ccttcgccgg aggtggaccc ccctaggtcg ttgacgacga ccttggcgcc
     1861 gcgtgcggcc agtgcgagcg catgggaacg gccgagcccc tgacccgctc cggtcacgat
     1921 cgcgacacgc ccgtcaaacc tcagttcttt gctcatactt cctcgacttc agaaacaatc
     1981 agagctgact gtttatgcga aatcgcgccg ctgtcaatcc cgcgactgcc cgatgtattt
     2041 caggcccgcg gtttgccgag gacgg
//
LOCUS       DASRZF010000391         5902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3395904, whole genome shotgun sequence.
ACCESSION   DASRZF010000391 DASRZF010000000
VERSION     DASRZF010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 5902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5902
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3395904"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="VFG22_17255"
     CDS             <1..566
                     /locus_tag="VFG22_17255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418046.1"
                     /translation="YDTGYYYDSLVSGVAYSTETEDGTVRTGVTGEDGDPGSFDYYQE
                     NATVSFSIGDTALGQSTAKDKVTPFDMAGVAEEAVGGCDVSGTLPEDDFRIVHNLAVL
                     LQTMDTDGDPSNGIIIGTDVAALFEGVSIDVDQSWEAFQADTDLLGVLEAANADNLLG
                     EETRSLVAREDALQALYEGIGLCPAAS"
     gene            969..1772
                     /locus_tag="VFG22_17260"
     CDS             969..1772
                     /locus_tag="VFG22_17260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418047.1"
                     /translation="MNRVLHFCSLTLLFALVACAGDDPSPSPGGGGTGGSGAEFPAAG
                     PASVVREDTDAFVYFFPADLDAETIRPALVWFNGASGYQEDFNYNGLLESVASWGFIV
                     IGGKSPGMNPEESDERSALLRRNEDPADVLFGQVDVARIALAGHSLGGFQTTESSSQY
                     RVAVAIQGAGTPTSSEAAPTLFMTSEGDEVVPSSIVITAFERAADDAWLAVHVSADHN
                     DPRTDGGVYREPLTAFLCWRLYDDDNGASWFVGDDCVLCNDADWGYETR"
     gene            1769..2662
                     /locus_tag="VFG22_17265"
     CDS             1769..2662
                     /locus_tag="VFG22_17265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HET6418048.1"
                     /translation="MSDSFYTIVNRAAGAGRCARLAPGALESLEARGLKLEVVFTDAP
                     KHATELARRKAESGGRRFLSVGGDGTAAEVVNGLVAAGVSSESELAMLPLGTGNSFLR
                     DFDISDVEAASAAIERGVSRPLDVLRLAHRDGVIHFINTMGTGFVARAGELTNERFKF
                     LGAAGYIAAVFVCVIRLQYEDNPLRYADRSDDSRTVLSSFSNSKFTGGSMKMAPDAEV
                     DDGLLDVVRAGPLGRAALISAFARIFSGTHTALDEVWTLQVPRVEFGNPSLQAVLIDG
                     DLYHLTPLSIDVLPNAIRLIA"
     gene            2659..3396
                     /locus_tag="VFG22_17270"
     CDS             2659..3396
                     /locus_tag="VFG22_17270"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET6418049.1"
                     /translation="MTETTTIWDRIVSLLIWILAGGFLVLVLSFLTLLYQFVRPDRTQ
                     ALSRWYCRTQLALTFNRQVYRVHSGVDPRTQYIFIQNHSSHLDHVAMYNATPHFKQGI
                     ELESHFDYPFYGWFMKARGTIPVSKDRARALESLRESIRAELDAGHSILGFPEGHRTE
                     TGRVGPFYDGLFRIAIELGVPVVPVAVTGLYEVLHKGSLVIRPGYEITVFVDEPIETR
                     DLSSEDVCDLRDRVRDTISAHVDAHFS"
     gene            complement(3450..4451)
                     /gene="dctP"
                     /locus_tag="VFG22_17275"
     CDS             complement(3450..4451)
                     /gene="dctP"
                     /locus_tag="VFG22_17275"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HET6418050.1"
                     /translation="MNRHFPFVLFLALVLFGLSGSVPSSSADEAVTLRIASLAPAGSS
                     WMKVLNAWKKTLEDETEGRLKLRFYPGGSQGDERDFVRKMRVGQLDGGVVTMTGMSML
                     VRPMVILVLPGFLDTYEKLDRVRLKMADQFEKMFQDEGFMMVAWGDAGKTRLFSQNKI
                     ERPSDIKSMRPWMWKDDLVFIEFYDVIGANGVRLGVPEVYPGLQTKMVDVVTSSALTA
                     VALQWYTRVKYMTGHNSAIIAGGMVMRKEKIDSLPPDLREAFLESSKRVETLLNKTIR
                     RDDQKAYEVVLKKGIIATDTSKYQAEWDAAAKEVRDRMTGRVFSKELLQAVAAAAAE"
     gene            complement(4475..5392)
                     /locus_tag="VFG22_17280"
     CDS             complement(4475..5392)
                     /locus_tag="VFG22_17280"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6418051.1"
                     /translation="MARDEGSALRERVADRAVMITGASSGIGEALAYRLGAAGAKVLL
                     VARSLEKLEVIQQGIEAHGGRAFVYAADLSDAKDTERLVAAVLAEHGRVDVLVNNAGI
                     SIRRSVQKSYERVHDFERTLELNFLGAVRLIMGFLPGMCAAKQGQILNVSTIGVQVNV
                     PRYGAYIASKAALDAFSRVLAVEALKDGVKVTTIYMPLVKTPMMKSTTIYDAFPMRTA
                     DEAADLIVDGIVRQPKRVATPVGNLFEFAYGLAPNAIDRVLNAAYQLYPESGDKKDQR
                     EPASGDAAMFQRVFKAVTRRAKRRRAGRH"
     gene            complement(5480..>5902)
                     /locus_tag="VFG22_17285"
     CDS             complement(5480..>5902)
                     /locus_tag="VFG22_17285"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET6418052.1"
                     /translation="QDYGVLIDTLECATSWDQLPKVHEHVRAYVKSRPQTVCMTHMSH
                     CYPQGANLYFIFIAKMDHEEFVEFHRGMLDAVQSSGATMSHHHGIGKMTAPWLEGQIG
                     SNELEVLRALKRHFDPKNVLNPGGTLALDLPDEQRRMP"
BASE COUNT         1069 a         1933 c         1771 g         1129 t
ORIGIN      
        1 cgtacgacac cggctactac tacgacagcc tcgtctcggg cgtcgcctac tccaccgaga
       61 ccgaggacgg cacggtccga accggcgtga ccggcgaaga cggcgatcct ggtagcttcg
      121 attattacca ggagaacgcc actgtctcgt tctccattgg cgacaccgca ctcgggcaga
      181 gcaccgccaa agacaaggtc acacccttcg atatggccgg cgtcgcagaa gaagcggtcg
      241 gcggatgtga cgtcagtggg acccttcccg aggacgactt ccggatcgtg cacaacctcg
      301 cggttcttct tcagacgatg gataccgacg gagaccccag caacgggatc atcatcggca
      361 ccgacgttgc cgccctcttc gaaggcgtga gcatcgacgt ggatcagagc tgggaagcct
      421 tccaagccga caccgacctg ctcggcgtcc tcgaagcggc gaacgccgac aacctcctcg
      481 gcgaggaaac acgcagcctc gtcgcgcgcg aggacgcgtt gcaggcactc tacgaaggca
      541 tcgggctctg cccggcggca tcctgagcta gggccagcca ggcgcggacc tcgacgagcc
      601 ctgggacatc gcatggcttc caaacggcac gcttctgctc agcgaacgaa ccggccaact
      661 caaggtgttc gtcgacggcg tcggtgaacc gccttcggtg attccgatca cggacgtggt
      721 cgcgcgtggt gaaggcggcg tgatgggcct cgaggtcgac ccggatgttg cgagcaacgg
      781 cttcatctgt gtatgcacca cctcggccgc cggccgcgct ttggtcctga cagcttcctc
      841 tgggtgggtg ccggggacgc cgcgatcggc gcaaactcac aagacgacgc ttcgctcggc
      901 ggcaaggtgc tccgcgtgag accggtaccg attgcgcctt tccgttgatc atgcacgctg
      961 aggtccagat gaatcgtgtt cttcacttct gttcgctcac cctcctcttc gcgctcgtcg
     1021 cctgcgcggg cgacgacccg agcccatccc caggtggcgg gggcactggc ggcagcggtg
     1081 ccgagttccc cgcggccggc cccgcatcgg tcgtgcgcga ggacaccgac gcgttcgtgt
     1141 acttcttccc ggccgacttg gacgccgaaa ccatccggcc cgcgctggta tggttcaacg
     1201 gggcgagcgg ctaccaagag gatttcaact acaacgggct tctcgagtcc gtggcatcct
     1261 ggggcttcat cgtcatcggt ggaaagtcac caggaatgaa tcccgaggag agcgacgagc
     1321 gcagcgcgct ccttcgacga aacgaggatc ccgctgacgt actgttcggc caggtcgacg
     1381 tggctcgaat cgcgctcgca gggcattcgc tcggcggctt ccagaccacg gaaagctcct
     1441 cgcagtatcg agtcgcggtc gccatacaag gcgcgggaac cccaacgagc agcgaggccg
     1501 cacctaccct cttcatgacg tccgaaggcg acgaggtcgt gccttcttcg atcgtcatca
     1561 ccgcgttcga gcgtgctgcc gacgacgcct ggctcgccgt ccacgtgagc gcggaccaca
     1621 acgatcctcg gacggacggc ggcgtctatc gcgagccgct caccgccttc ctctgctggc
     1681 ggttgtacga cgacgacaac ggtgcgtcct ggttcgtcgg cgatgattgc gtgctctgca
     1741 acgacgccga ctggggttac gaaacgcggt gagcgattcc ttctatacca tcgtcaatcg
     1801 agcggcaggc gctggacgat gcgcgaggct cgccccaggc gcgctcgaat cgctcgaagc
     1861 gagggggttg aagctcgagg tcgtgttcac cgacgcgccc aagcacgcca ccgagctcgc
     1921 ccggcgcaag gccgagagcg gcggccggcg tttcctctcc gtcggcggtg acggaaccgc
     1981 tgcagaggtc gtcaatggcc tcgtggccgc aggcgtctcg agcgagtcgg agctcgccat
     2041 gctgcccctc ggtacgggca actcctttct gcgggacttc gacatcagcg acgtcgaagc
     2101 cgcttcggct gcaatcgaac gtggggtcag cagaccgctc gacgtgcttc gcctcgcgca
     2161 ccgagacggc gtgattcact tcatcaacac catgggcacc ggcttcgtgg cgcgcgcggg
     2221 tgagctcacc aacgagcgat tcaagttcct cggcgcggcc ggatacatcg cagccgtttt
     2281 cgtctgcgtc attcgcctcc agtacgagga caatccactt cgatacgcag accgttccga
     2341 tgattcccgg accgtgctct cctcgttttc gaacagcaag ttcaccggtg gctcgatgaa
     2401 gatggctccg gatgccgagg tcgacgacgg cctgctcgac gtcgtgagag cgggcccctt
     2461 aggacgcgcg gccctgattt cggcattcgc ccgcatcttc tctggaaccc acaccgcgct
     2521 cgacgaggtc tggactctgc aagtgccgcg cgtcgagttc ggcaatccat cgctgcaagc
     2581 ggtgctcatc gacggtgacc tctatcacct cactccgctc tccatcgacg tgctgcccaa
     2641 tgcgattcgc ctcatcgcat gaccgagacc accacaatct gggatcgaat cgtcagtctg
     2701 ctgatctgga tcctagcggg cggattcttg gtccttgtgc tgtcgtttct caccctgctc
     2761 taccagttcg tgagacccga ccgcacgcaa gctctctcac gatggtactg caggacccaa
     2821 ctcgcattga cgttcaatcg gcaagtttat cgggtacact caggggtcga ccctaggact
     2881 cagtacatct tcattcagaa tcactcgagc cacctggatc acgtcgcgat gtacaacgcg
     2941 acgccgcact tcaagcaggg catcgagctc gaatcccact tcgactatcc gttctatggc
     3001 tggttcatga aggcgcgcgg cacgattccc gtgtcgaagg accgggcacg cgcgctcgaa
     3061 tcactgcgag agtcgatccg agccgagctc gacgcgggcc actcgatcct gggcttccct
     3121 gaggggcacc gcaccgagac gggtcgcgtc ggtccatttt acgatggctt gtttcgcatc
     3181 gcgatcgagc ttggcgtccc ggtggtgccg gtggcggtca cgggtctcta cgaggtgctc
     3241 cataaagggt cgctggtgat ccgacccggg tatgagatca cggtcttcgt cgatgagccc
     3301 atcgaaacgc gagacctcag cagcgaagac gtctgcgacc tacgagatcg ggtccgggac
     3361 accatttcgg cccacgtcga tgcgcatttc agctgaacgc tgcgctgcca ctgctcacaa
     3421 cattcgtgag cctttccacg tcgagcgggc tactcggcgg cggccgctgc aacggcctgc
     3481 agcagctcct tggaaaagac ccggccagtc atccgatcgc gaacttcctt ggccgcagcg
     3541 tcccattcgg cttgatactt gctggtgtcg gtcgcgatga tgcccttctt gagcaccacc
     3601 tcataggcct tctgatcgtc acggcgaatc gtcttgttga gcagtgtctc gacccgcttc
     3661 gacgattcca ggaacgcctc ccgaaggtcc ggcggcagac tgtcgatctt ctccttacgc
     3721 atgaccatgc cccccgcgat gatcgcggag ttgtggccgg tcatgtattt gacgcgcgtg
     3781 taccattgca gcgcgaccgc cgtcagcgcc gacgaggtca ccacgtcgac catcttcgtt
     3841 tgaagcccag ggtagacctc gggcacaccg aggcgtaccc cgttcgcacc gatcacatca
     3901 tagaactcga tgaagaccag gtcgtctttc cacatccacg gacgcatcga cttgatgtcc
     3961 gaggggcgct cgatcttgtt ctgcgaaaag agcctggtct ttccggcatc gccccaggcg
     4021 accatcatga agccttcatc ttggaacatc ttttcgaact gatcggccat cttcaagcgc
     4081 acgcggtcga gcttttcgta ggtgtcgagg aagccgggca gaaccaggat gaccatcggt
     4141 cgaaccagca tgctcattcc ggtcatggtg acgacaccgc cgtcgagctg tccgacgcgc
     4201 atcttgcgga cgaagtcacg ttcatctccc tgcgagccgc ccgggtagaa tcggagcttg
     4261 agccggccct cggtctcgtc ttcgagggtc ttcttccaag cgttgaggac cttcatccag
     4321 gacgagccgg cgggggcgag cgatgcgatt cgtagggtca cagcctcgtc ggccgagctg
     4381 ctcggaaccg agccggaaag cccgaaaagc acgagcgcca ggaacagcac aaacgggaaa
     4441 tgtctattca cgataagggt atagccccag cgccctagtg tcgtccagcc ctgcggcgct
     4501 tcgctctgcg cgtcaccgct ttgaagaccc gctggaacat cgccgcatcc cctgatgccg
     4561 gctcgcgctg gtccttcttg tcgcccgact ccggatacag ctggtaggcc gcgttcaaga
     4621 cccggtcgat ggcgtttgga gccaggccgt acgcaaactc gaacaaattg ccgaccgggg
     4681 tcgccacacg cttgggctgg cggacgatac cgtcgacgat caggtccgcc gcctcatcgg
     4741 ccgtacgcat cggaaacgca tcgtagatgg tcgtcgactt catcatcgga gtcttcacga
     4801 gcggcatgta gatcgtcgtg actttgacgc cgtccttcag ggcctccacc gcgagcacgc
     4861 ggctgaacgc gtcgagcgcc gccttgctgg cgatgtacgc tccgtaacgc gggacattca
     4921 cttgaacgcc gatcgtcgag acgttgagaa tctgaccttg cttggccgcg cacattcccg
     4981 gcaggaagcc catgatcagg cgtacggctc cgaggaagtt gagctccagc gtccgctcga
     5041 agtcgtgtac gcgctcgtat gacttctgaa cgctacgtcg aatcgagatc ccagcgttgt
     5101 tgacgaggac gtcgacccgt ccgtgctcgg cgagaaccgc cgccaccaag cgctcggtgt
     5161 ccttcgcatc ggagagatcc gcggcgtaca cgaacgcgcg gcccccatgc gcctcgatgc
     5221 cttgctgaat cacctcgagc ttctcgagag accgggcgac gagaagaacc tttgcgcccg
     5281 cggcacccag acgatacgca agcgcttcgc cgattccgct cgaagcgccg gtaatcatga
     5341 ccgcgcggtc cgccacgcgc tcgcgcaagg cgcttccttc atctctcgcc atgcgcgaag
     5401 cctagccgaa atgcgggttc cggtctcagg tttcaggttt ccctggcgag caaccgaacc
     5461 gcgatctctt cggccgcgct caaggcatcc ggcgttgctc gtcgggcagg tcgagcgcga
     5521 gcgtgccgcc ggggttcagc acgttcttcg gatcgaagtg gcgcttgagc gcacggagca
     5581 cttcgagctc gttgctgccg atctggccct cgagccaggg cgccgtcatc tttccgatgc
     5641 cgtgatgatg gctcatcgtg gcgccggagc tttgaaccgc atcgagcatc ccgcggtgga
     5701 actcgacgaa ctcctcgtga tccattttcg cgatgaagat gaagtagaga ttcgcaccct
     5761 gcggatagca gtgtgacatg tgcgtcatgc agaccgtctg cggacgactc ttcacataag
     5821 cgcggacgtg ctcatgcacc ttgggaagtt gatcccagct cgtcgcgcat tcgagcgtgt
     5881 cgatcaggac gccgtaatcc tg
//
LOCUS       DASRZF010000392         2231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_989551, whole genome shotgun sequence.
ACCESSION   DASRZF010000392 DASRZF010000000
VERSION     DASRZF010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989551"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VFG22_17290"
     CDS             complement(<1..266)
                     /locus_tag="VFG22_17290"
                     /inference="COORDINATES: protein motif:HMM:NF020921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418053.1"
                     /translation="MSNISERAMSKEDIDSIDAYWRACNYLAAGMIYLQANPLLREPL
                     RPEHIKSRLLGHWGASPGLSFMYVHLNRLIRMHDLNAIFLAGPG"
     gene            complement(388..1614)
                     /locus_tag="VFG22_17295"
     CDS             complement(388..1614)
                     /locus_tag="VFG22_17295"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HET6418054.1"
                     /translation="MNVLVLNSGSSSLKYQLFDMGQRALLAGGVAERIGESGSRLVHV
                     QRDRSGKLREETKSGDLSDHAEAIDRIAGLLADAGVAWDASELLGIGHRVVHGGEAFR
                     EPCLIDARVIETIRRLSSLAPLHNPANLLGIEVAMARQPNVPQVAVFDTAFHHSIPPR
                     AHRYAVPEEWYQSHGVRRYGFHGTSCAYVAKQAARELGRPLEELNLIVLHLGNGASVT
                     AIERGKSVDTSMGMTPLEGLVMGTRSGDIDPGVIVHLALQAGMSVEKIDADLNGESGL
                     FGLCGVGDVRDVLGREAAGDARARLALDVYTYRMRKYIGAYMAVLGHVDAIVFTAGVG
                     EHSADIRERACEGLEGLGVQLDEARNRAGAKGIRPIHRRGAPLEVLVVPTNEELEIAE
                     QTLECIEKNPVEFDGE"
     gene            1799..1927
                     /locus_tag="VFG22_17300"
     CDS             1799..1927
                     /locus_tag="VFG22_17300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418055.1"
                     /translation="MFVVADGAPGQSASAPWVAVQELTHAEGHPNDAKLERILRFH"
     gene            complement(1971..2102)
                     /locus_tag="VFG22_17305"
     CDS             complement(1971..2102)
                     /locus_tag="VFG22_17305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418056.1"
                     /translation="MIPKATKRTELGLLVALLTARHARVERGNRASPTQHAQLRMDY"
BASE COUNT          389 a          712 c          677 g          453 t
ORIGIN      
        1 tggccgggtc ccgcgaggaa gatcgcattc agatcgtgca tccgaatgag ccggttcagg
       61 tgcacgtaca tgaacgagag accgggactt gcgccccagt ggccgagcag ccggctcttg
      121 atgtgctcag gcctgagcgg ctcgcggagg agcgggttcg cctgaagata gatcatgccg
      181 gcggccaggt agttgcatgc ccgccagtag gcatcgatgc tgtcgatgtc ctctttcgac
      241 atcgcccgtt ctgaaatgtt gctcatttgt atctttctcc gtttccgtct cggtcctgcc
      301 tggcccgggg gcccgtggtt ggcatgcctt agcgctgaca acatctgacg cgcgagttcg
      361 acgaccgatc actccgtcac ggcgcgttca ttcgccgtca aactcgacag gatttttttc
      421 aatgcactct agggtttgct cggcgatctc cagctcctcg ttggttggga cgaccagcac
      481 ctccagcgga gcgcctcgac ggtggatcgg tcggatgccc ttcgcgccgg cccggttgcg
      541 cgcttcatcg agttgcacac cgagcccctc cagcccctcg caggcgcgct cgcggatgtc
      601 ggctgagtgt tcgcccacgc ccgcagtgaa gacgatcgca tcgacgtggc ctagcactgc
      661 catgtaggcc ccgatgtact tgcgcatgcg gtaggtatag acgtccaggg cgagccgagc
      721 tcgcgcgtcg cccgctgctt cgcgccctag gacgtctcgc acgtcaccga ctccgcacaa
      781 cccgaagagc ccgctctccc cgttgaggtc cgcgtcgatc ttctcgacgc tcatccccgc
      841 ctgaagcgcc aggtggacga tcactccggg atcgatgtca ccactgcgcg tgcccatcac
      901 caggccctcg aggggcgtca tgcccatgga ggtgtcaacg ctcttccctc tctcgatggc
      961 ggtcacgctc gcaccgttcc ccaggtgcag aacgatcaaa ttgagctctt cgagcggccg
     1021 accgagctcg cgagccgcct gcttcgccac ataggcacac gaggtgccgt ggaacccata
     1081 gcgacgcaca ccatgagatt ggtaccactc ctcggggacc gcgtatcgat gggctcgcgg
     1141 cggaatgctg tggtgaaagg cggtatcgaa gaccgccacc tgcggcacat tcggttgtcg
     1201 tgccatggcc acctcgatgc ccaacaggtt ggccggattg tgcagcggcg caagggacga
     1261 gagccgccga atggtctcga tcacacgtgc gtcgatcaaa cagggttcgc gaaaggcttc
     1321 acccccgtga acgacccggt gtccgattcc gagcagctcg cttgcatccc aagccactcc
     1381 cgcgtcagcg agcagacctg cgattctgtc gatagcttct gcatggtccg agaggtcgcc
     1441 ggatttggtt tcctcgcgaa gctttccgga ccgatcccgt tggacgtgca cgagtcggct
     1501 gcccgactcg ccgatgcgtt cggccacgcc tcctgcgagc agagcccgct ggcccatgtc
     1561 gaagagctgg tacttgagtg aggagcttcc ggagttgaga actagaacgt tcacgacctg
     1621 ctcttgtacg tcctcagaag atttcgaaca agagggcacg cttgctggga gacggttcat
     1681 ggtttcggcc caggccgcat tggaggtttg atcttgtccc tttcgaacag aacatccctg
     1741 tgtcgaccac agtacacgtc gaccgggtgc agtcgtcgag cgactcgtgg tagccgcagt
     1801 gtttgtagta gctgacggcg cgccgggcca gagcgccagt gcgccctggg tcgccgtgca
     1861 ggagctgaca catgcggaag ggcaccccaa cgacgcgaag ttggaacgaa ttctccgctt
     1921 tcattgaaga gtgaaacgag cttcggaggg cagcatgacc ggcttcgaca tcagtagtcc
     1981 atgcgcagct gcgcgtgctg cgtcgggctc gcgcggttgc cgcgctcgac tcgcgcatgt
     2041 cgagccgtca gcaacgccac taagagtccg agctctgtcc gtttggtcgc cttcgggatc
     2101 atgaaggctc acatcgcgcg acggacagcg ttctcagaaa cccgagcctt caacgcgtgc
     2161 tagattctgg tcgcctgata tggtggcctc gcaactcttc cgaacgcttt cgctggctct
     2221 gatttcttcg g
//
LOCUS       DASRZF010000393        13680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_5108082, whole genome shotgun sequence.
ACCESSION   DASRZF010000393 DASRZF010000000
VERSION     DASRZF010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 13680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13680
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5108082"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1134)
                     /locus_tag="VFG22_17310"
     CDS             complement(<1..1134)
                     /locus_tag="VFG22_17310"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HET6418057.1"
                     /translation="MQDRPALFPGRPSELGCTIRDVDPGAANRKNDRWRALDLLRLVA
                     VVLMVQGHVFTALLDRGLRHGPWYIRHSYVHGLTAPIFFFASGLAFGVVTLRNWSDHT
                     HWGPSAKRRFARYGWLLFIGYMLSLPGLSFRELWTSASPDTVRMFFRVDALENIAVSL
                     ILVQGLVWLTKKQRPFIGTVTVLGSALVLSAPAVWRLPIERWLPMGVAAYINVDTGSI
                     FPLFPWTGFLCAGVVTAYLSVDPHRGSLRRRLGWGLATTGGLLWLAARLLQDAGADPS
                     GNQDYWKTSPIFFLTRLGLILLGFAALCALESLWARRCAAPAVASTPGNAMGLLQRVG
                     QETLVIYVIHLLLLYGSPISPGLNTLLGRELNLQGASIVAALVL"
     gene            1178..2329
                     /locus_tag="VFG22_17315"
     CDS             1178..2329
                     /locus_tag="VFG22_17315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6418058.1"
                     /translation="MSYIETEEQQLLRQQVREIGRKYGREYIAKKGREGLSPDELWDE
                     VARQGFIGVNTPEEYGGGGLGIYELAIVCEELAAAGCPLLLLVVSPAICATLIARFGT
                     EAQKVRWLPRLASGEMKMAFAITEPNAGSNSHRLETAAVKDGEDWVLNGTKYYISGAD
                     DAEAILVVARTGGAVDGRGRLSLFIVDSNAPGLEKTLIPLQIQGADRQFTLFFDNVVV
                     SQDRLLGNEGQGLSQVFVGLNPERILIAALANGAALHAIEQASRYANERAVWGKPIGT
                     HQGIAHPLAESKIEVELARLMTMKAAWLFDHGKDAGEAANMAKFAAGEAATRAVDTAI
                     QVHGGNGMSEEYGLVLLWGLARTLRIAPVSREMILNFVAQHTLDLPKSY"
     gene            2387..2518
                     /locus_tag="VFG22_17320"
     CDS             2387..2518
                     /locus_tag="VFG22_17320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418059.1"
                     /translation="MSKDHKGTDTKNRPKLTAKEKKAKKAEKAAKAAGKAGLGIPHS"
     gene            complement(2571..2768)
                     /locus_tag="VFG22_17325"
     CDS             complement(2571..2768)
                     /locus_tag="VFG22_17325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HET6418060.1"
                     /translation="MAEGTIKRLTDRGFGFIETSPGNDIFFHMSNVDGVRFEDLREGQ
                     KVSFDEGQGPKGPRAENVRPL"
     gene            complement(2964..4676)
                     /locus_tag="VFG22_17330"
     CDS             complement(2964..4676)
                     /locus_tag="VFG22_17330"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: protein motif:HMM:NF010052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HET6418061.1"
                     /translation="MKGETDDQKLGGEDRIETRKRGVWLLKNPSTNKGLAFTKEERRQ
                     LGLDGLLPDQVLEIEQQVQLELEHIRAKSTDLEKYIGLTSLQDLNEVLFYRVLVENLP
                     ELLPIVYTPTVGLACQKFSHILRSARGIWITPSNIERVHEVLESAPFHDIRLLVVTDN
                     ERILGLGDLGAGGMGIPVGKLALYVAGAGIHPSKCLPVSLDVGTNNQELLNDPLYNGY
                     RGRRLTGETYFEFVERFVQAARDVFPRAIIQWEDFAKTHSFSLLEHYQKRVPSFNDDI
                     QGTAAVTLGGILAALRITGQAIRDQRILFIGAGGACIGIARLTSAAMREAGATDHEIA
                     RSIAAFDSQGLLHAEREIKEPFKREFALSKKSVEHYGLTQSSLTNPVEMIRAFKPTVL
                     VGATAQAGAFTRPMIEEMAKHVDRPLILPLSNPTSKSECTPQDAFEWTAGRALVATGS
                     PYDDVTYEGKRHVIGQSNNVFIFPGVGLGAIVSETREINDEMFLIAAQTLASCVSEER
                     LALGAIFPRESDLREVSFQIACAVVRYARDAHLGRVIPDEEIEATIRSGVWYPSYIPI
                     LAQR"
     gene            complement(4854..5801)
                     /locus_tag="VFG22_17335"
     CDS             complement(4854..5801)
                     /locus_tag="VFG22_17335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HET6418062.1"
                     /translation="MSIAYITHPSFMLHEMGAHHPEQPARLGAINDRLLSSGLNMALH
                     HYDAPRAEREHLLRVHDAAYIDRVFGASPREGLTWLDGDTAMNPYTLEAALHASGAAV
                     QAVDLVMAGDAQRAFCAVRPPGHHAERARAMGFCFFNNVAVGAAHALAAHGLERVLIA
                     DFDVHHGNGTEDIFGGDERVLFCSTFQHPFYPHSGCDTRAPNVVNVPLPAQTDAGAFR
                     EAVSTYWLPAIDAFEPQLVMISAGFDAHVEDDMANLRLHERDYAWITRKLCERADRYA
                     EGRIVSSLEGGYALGALGRSVAVHIKEFLEDRRDGMTCP"
     gene            5948..8629
                     /locus_tag="VFG22_17340"
     CDS             5948..8629
                     /locus_tag="VFG22_17340"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HET6418063.1"
                     /translation="MKKHYLLKVFEPTSVAVIGASDRESSVGGQLLRNIREGGFTGDI
                     YAVNPKRTTVQGLKSHASLSAIDRPVDLAVIAIPAKNIPDVMRECGNLGVGAAIVLSA
                     GFAEVGKAGTDLQSEIVDIARTHNIALVGPNCLGIVRPRVGLNATFAKSPARQGHVAL
                     VAQSGAFCTALLDWADTKGFGFSAVASLGATADVGFGEVLDYLAVDPETRSILLYVEG
                     VTNARSFMSGLRAAARLKPVIVVKSGRSDSGTRAAVSHTGALAGADRVFDAALRRAGA
                     VRVSSVSELFAAAQLLASGARVAGPRLAILTNGGGPGVMAADRAADLDVPLAELSVAT
                     VEQLGKVLPAHWSHSDPVDILGDADPERYRAATEIVLADPSVDGLLVLLTPQGMTDPT
                     GCADAVIDSSRESTKPVLACWMGAGLVNEGRQKFAEADIPQFRAPEAGVEAFGYLACY
                     RRNQTALLQAPGPMSRHKAPDVEGARLIIEHVLGERRQTLSSAEAKAVLHAFQIPTSP
                     SINVASAAEALVAAEGVGLPIAMKINSPDITHKSDVGGVRLNIREPRSVRTAFREMMD
                     SVRAQRPDARIDGVTIEPMVDRPNAREIMIGIATDPIFGPVISFGAGGTAVEIFADSN
                     VALPPLNEYLSRELIKGTRAARFLKHFRNLPEANEEKLIDVMQRVSEIACELREVREL
                     DINPLLVDEDGVIAVDARIVVAASVASTAHYGHMAIHPYPAELVTRWQLSDGNDVVIR
                     PIRPEDATMEQAFVQSLSAESKYFRFMQNLDKLTPEMLARFTQIDYDREMALVGVLED
                     GSSRAQMIGVARYVSNPDRHSCEFGLTVGDEYQRQGIGRELMRRLMAVARDRGIDVME
                     GEVLSKNTKMLTLCERLGFRVARDPEDPDLVQVRRHL"
     gene            8742..10376
                     /locus_tag="VFG22_17345"
     CDS             8742..10376
                     /locus_tag="VFG22_17345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HET6418064.1"
                     /translation="MGTLEGLDWVVVALYFIAVFGVAAWATVSERREQTSSADYFLAG
                     RNVGWFVIGASLFASNIGSEHLVGLAGTGAASGVAVGQFEILASLILLVLGWVFVPFY
                     LRSGVFTMPEFLERRYSPAARWYLAVISIVGYVLTKISVTIAAGGIVFESLMGIDFWT
                     GALVVVVATGIYTVLGGLRAVLYTDLLQMFVLVGGAVAVTWIGLDELGGWGAMVEGAG
                     PGFMSVWKPLSDPDFPWTGILLGAPILGVWYWCTDQFIVQRVLSAADEDNARRGTIFG
                     GLLKLLPLFIFVVPGVVAHALASRGEITLDSPDQALPILIGALLPRGIRGLVVAGLLA
                     ALMSSLSSVFNSCSTLFTRDIYLKLRPDASEARQVRVGQTATAALVALGLLWIPMMQF
                     ISGQLYQYLQSVQAYIAPPIAAVFLIGILWRRANAPGAIASLATGFVFGTLRLIAELT
                     KEEHEGFILAYAEINFLHFAALLFLVCSVVLVGVSLLTRAPDASKLDGLTFATTAPSE
                     NAQPSRWRTQDRWVSVLLVVLVGLVWFYFSPWVQAR"
     gene            complement(10346..10612)
                     /locus_tag="VFG22_17350"
     CDS             complement(10346..10612)
                     /locus_tag="VFG22_17350"
                     /inference="COORDINATES: protein motif:HMM:NF014147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418065.1"
                     /translation="MPGASFRIVASPWTAPPWMKDNGKYYDPGQRRGGRLLEGHYDTF
                     ARYFVAYLQAYRKAGVDIWALTPVNAPHRIASDSGHRACTHGLK"
     gene            10926..12368
                     /locus_tag="VFG22_17355"
     CDS             10926..12368
                     /locus_tag="VFG22_17355"
                     /inference="COORDINATES: protein motif:HMM:NF023165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HET6418066.1"
                     /translation="MTPNGLRVWLIPLVTISLASVLGVTPEAAAQDGDATPAAQPLGA
                     TDQTWNPEEMEALLAPIALYPDPVLTQVLVSSTNPQEVMDAGNWLVANPELEGDALDA
                     AAEAAGFTEPMRAVMPFRQIVDQMCLEMGWTTELGQAYTNDQPGVLAAVQRLRAQAQD
                     VGNLQSSEQMKVETKSQEGQQVIVIESPSPQIIYVPQYDPVTVYAPPPTTVVVEDKGP
                     SAGAMVATSMLSFGVGLAIGASTRPYYSRGYCYPNYYRGFVPPPPPYYYRPVYGGGYH
                     PSHHYNRSSNYNKVLSDNNVVVVNKNKDYWGGFDDKPRGKGRTDNVKSPITKARSDRP
                     ELKELERNSGKKSDWKGKSSYAGADPKARSKAKLPDKGNMPESKAVAKQAKANASAAK
                     ARKPSSGGDRGYAGKAKPGAASRPESGSKRSAAGPSSRPQRSPSSSGQGKLSGRGGGA
                     SSRTASARGRSSMPQGARTKGGGAAANRRR"
     gene            12378..13361
                     /locus_tag="VFG22_17360"
     CDS             12378..13361
                     /locus_tag="VFG22_17360"
                     /inference="COORDINATES: protein motif:HMM:NF022892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2950 domain-containing protein"
                     /protein_id="HET6418067.1"
                     /translation="MERTFAGMQKLPHRTSILLFALALASCTQKAEVEPQAQPKDESH
                     TSFPSAESAVEGLVAAVEAHDVAALEKIFGPGGESLVQWGDPVAENQDRARFLEMYRA
                     KHELVDGRDGEKVLEVGADDWPLPAPLVEKDGHWSFNGVEGVDELVYRRVGRNELGAI
                     RVAHGFVDAQYEYASKRRDGKIAGLFAPYLISDPGLHNGLYWPTERDEAPSPAGPFVA
                     AAAAEGYRKAAAGEPTPYHGYFYRMLYAQGADAPGGAMDYFEDGQIVRGFALLAWPSE
                     YGVSGVKTFIVNQDDIVYEKDLGDDTATAVNDIRIFNPDETWDQVRVEADQ"
BASE COUNT         2548 a         4324 c         4374 g         2434 t
ORIGIN      
        1 aagcacgagg gcggcgacga tcgatgcgcc ttggaggttc aactccctgc ctagaagcgt
       61 attgagaccg ggactgatgg gcgacccgta caaaagcaac aggtgaatga cgtagatgac
      121 gagggtttcc tggccgaccc gttgaaggag acccatcgcg ttcccaggcg tcgaagcgac
      181 ggcgggggcg gcgcacctgc gtgcccagag cgactcgaga gcacagagcg cggcgaaccc
      241 gagcaagatg aggcccaagc gcgtcaagaa gaagatcggg ctcgtcttcc agtagtcctg
      301 atttcctgac ggatccgcgc cggcgtcctg caagaggcgt gcagccagcc acaagaggcc
      361 ccccgtggta gccaagcccc agccaaggcg tcgccggagc gagccccgat gaggatcgac
      421 cgacaggtag gcggtgacca ccccggcgca gagaaaacca gtccagggaa acaaaggaaa
      481 gatcgacccc gtgtcgacgt tgatgtacgc ggcgacaccc atcggaagcc agcgttcgat
      541 cggaagccgc catactgcgg gcgcgctcaa aacgagggcc gacccgagaa ccgtgacggt
      601 ccctatgaac ggccgctgct tcttcgtgag ccagacgagc ccttgcacga ggatcagcga
      661 gaccgcgata ttttcgaggg cgtccacccg gaaaaacatc cggacggtgt cgggcgatgc
      721 ggacgtccac aactcccgaa agctcaagcc gggcaggctg agcatgtagc cgatgaagag
      781 caaccagcca taccgcgcga agcggcgctt tgccgatggg ccccagtgcg tatggtctga
      841 ccagtttcgg agggtgacga ccccaaaggc caacccggac gcaaagaaga atatcggcgc
      901 agtcaaccca tgaacgtaac tgtgtcggat gtaccacggc ccatgacgca gtcctctgtc
      961 gaggagcgcg gtgaacacgt ggccttgcac catgagcacc accgcaacga gtcgcagtag
     1021 gtcgagcgct cgccaacggt cgttctttcg attagccgca ccggggtcca cgtcgcgaat
     1081 cgtacatcca agctcgctcg ggcggcccgg aaagagagct ggccgatcct gcaatgctga
     1141 acaccacaaa gcgctcgcat tgtgatacga cccgcgaatg tcgtacatag agactgaaga
     1201 gcagcaactg ctgcggcaac aggttcggga gatcggacgc aagtacgggc gcgagtacat
     1261 tgcgaagaaa ggaagggaag ggctttcacc ggatgagctc tgggacgaag tggcgcggca
     1321 gggattcatc ggcgtcaaca cgcccgagga gtacggcgga ggaggtcttg ggatctacga
     1381 gctggcgatc gtctgtgaag agctggctgc ggccggctgt ccgttgctcc tcttggtcgt
     1441 gtctccggcg atctgcgcga cactgatcgc acgctttgga accgaagcac agaaggtgcg
     1501 ttggttgccc cggctcgcgt cgggcgaaat gaagatggcc ttcgccatca ccgagcccaa
     1561 cgccggctcc aactcgcatc ggctcgaaac ggcggctgtg aaggatggcg aggactgggt
     1621 tctcaacggg accaagtact acatctcggg cgccgatgat gcggaggcga tcctcgtcgt
     1681 agcgcggacc ggtggtgcag tggacggccg tggacgtctg tccctcttca tcgtcgattc
     1741 gaatgcccca ggtctcgaga aaacgctgat acctctgcag atccagggcg ccgaccgcca
     1801 gttcacgctc ttcttcgaca acgttgtcgt gagccaagac cggctgcttg ggaacgaagg
     1861 ccaggggctc tctcaagtct tcgtcggact caatcccgag cgcatcctaa ttgccgcgct
     1921 ggccaacggg gctgcgctcc atgcgatcga gcaggccagt cgctatgcga acgaacgtgc
     1981 cgtttggggc aagcctatcg ggacccatca aggcatcgcc catcccttgg ccgagtccaa
     2041 gatcgaggtg gagctcgcgc gcctgatgac catgaaggcc gcctggctct tcgatcacgg
     2101 caaggacgcc ggcgaggccg ccaacatggc caagtttgcg gctggcgaag cggcgacccg
     2161 agcggtcgac acggccattc aagtgcatgg cggaaacggc atgtccgaag agtacgggct
     2221 cgtgctgttg tggggcttgg cgcgcacgct gcgcatcgcg ccggtgagcc gcgagatgat
     2281 tctgaacttc gtggcgcagc acacgctcga cttgcccaag tcctactgag ctcatctcag
     2341 atgacatagg cgtttctcgg agcgaccgaa ccacaaaggg atctcgatga gcaaagatca
     2401 caagggaacc gacacgaaga acagaccgaa actcaccgcg aaagagaaga aggccaagaa
     2461 ggcagaaaag gccgccaagg cagcgggcaa ggcgggacta ggcattcccc acagctagag
     2521 gacagaccac gccctcgcgc gctgctctca tgcagatctg cggacaggcc tcaaaggggg
     2581 cggacgttct ccgcgcgtgg ccccttgggg ccctggcctt cgtcgaatga gaccttctgt
     2641 ccctcgcgca gatcctcaaa gcgcacgcca tccacgttcg acatgtggaa aaagatgtcg
     2701 ttgccgggag aggtttcgat gaaaccgaaa cctcggtcgg taagacgttt gatggtacct
     2761 tcagccatgt tgcattcgtt cctattcgag agcgttgtgc ccgtgatagc tctattcgcc
     2821 cctcggcgcc attcggtgag cacgcaagct ggttcggccg caattgagaa gccgatgagg
     2881 tcgattgcgt cgtgtgcggg gcgtgctgcg cgtggtagag cggccaggcg cgcccgggga
     2941 ttcgtcaccg agaacttgcc ccgttagcgc tgggcgagga tcgggatgta gctcgggtac
     3001 cagaccccgc tgcggatggt tgcctcgatc tcctcgtcgg ggatcactcg cccgagatgt
     3061 gcgtcgcgtg cgtagcgcac gacggcacac gcgatctgga agctcacttc gcgcagatcg
     3121 ctctcacgcg ggaagatggc gcccagcgca aggcgctctt cactgacgca gctggccagg
     3181 gtctgcgcag cgatcaagaa catttcgtcg ttgatttccc gtgtctccga gacgatggct
     3241 cccaggccca ccccggggaa gatgaagacg ttgttgctct gcccgatcac atgccgctta
     3301 ccttcgtagg ttacgtcgtc gtagggacta cccgtggcca cgagcgctcg gccagccgtc
     3361 cattcgaagg cgtcttgcgg agtgcactcc gacttcgatg tggggttgga gagtggcagg
     3421 atgaggggcc gatcaacgtg cttggccatt tcctcgatca tcggccgggt gaaggcgccc
     3481 gcctgagctg tcgcgcccac gagcacggtc ggcttgaagg cgcgaatcat ctcgacgggg
     3541 tttgtgaggg agctctgcgt aagcccatag tgttcaacgc tcttcttgct gagcgcgaac
     3601 tcgcgcttga acggctcctt gatctcgcgc tcggcgtgca gcagaccttg tgagtcgaac
     3661 gcagcgattg atcgtgcgat ctcgtggtcg gtggctcccg cctcgcgcat ggccgccgac
     3721 gtcaagcggg cgatgccgat gcacgcgcca cctgctccga tgaagagaat tcgctggtcg
     3781 cggatcgcct gcccggtgat ccgaagcgcc gcaaggatgc cacccagcgt caccgcggcg
     3841 gtcccctgga tgtcgtcgtt gaagctcgga acgcgcttct gatagtgctc gagcaggctg
     3901 aatgagtgcg tcttcgcgaa gtcctcccac tgaatgatgg cgcggggaaa cacgtcacgc
     3961 gcagcctgca cgaagcgctc gacgaactcg aagtaagtct cgcctgtcag ccggcgtccg
     4021 cgataaccat tgtagagtgg gtcgttcagc agttcttggt tgttcgtgcc cacgtcgagg
     4081 ctcacaggaa gacactttga cgggtgaatg ccggcgccag ccacgtagag tgccagcttg
     4141 ccgacgggaa tccccattcc cccggcaccg aggtcgccca gccccaggat gcgctcgttg
     4201 tcggtgacta cgagcaggcg gatgtcgtgg aagggcgcgc tctccaagac ctcgtgcacg
     4261 cgctcgatat tgctaggggt gatccagatg ccgcgcgctg accggagaat gtgactgaac
     4321 ttctggcagg ccagccctac ggtcggcgta tagacgatgg gaagcagctc tggcaggttt
     4381 tcgaccaaca cacggtagaa gagcacttcg ttcaggtctt gcaggctcgt gagcccaatg
     4441 tacttctcca ggtcagtcga ctttgcgcgg atgtgctcca gctctagctg gacctgctgc
     4501 tcgatttcga gcacctggtc cggcagcaga ccgtcgaggc cgagctgtct gcgctcctct
     4561 ttcgtaaagg ccagcccttt gttcgtggat gggttcttga gcagccaaac tcctcgcttg
     4621 cgagtctcga tgcgatcttc cccgccgagc ttctgatcgt cggtttcacc cttcactgcg
     4681 tgccctcccg gtacggcgct agcctagcac ttctgacatg gaacccacgg agtggcgtat
     4741 tcaactgtgt ctgcgggtac tctctgcgca acgaaacgag caaatcgcgt gatggcattc
     4801 cgcccgggga tccgcgctga cgtggagcac ggcatttgcc atggtcggag atttcagggg
     4861 caggtcatac cgtcgcggcg gtcttccaag aactccttga tgtgcaccgc gacgctgcgc
     4921 ccgagcgcgc ccaaagcata tccgccctcc agactagaca cgatgcgccc ttctgcgtaa
     4981 cggtccgcgc gctcgcacag cttgcgcgtg atccacgcat agtcgcgctc gtgcagtcgc
     5041 agattggcca tgtcgtcttc gacatgcgca tcgaatccgg ccgagatcat caccaattgg
     5101 ggctcgaaag cgtcaatggc gggcagccag taggtcgaca cggcctcgcg aaatgcgccc
     5161 gcgtctgttt gcgccggcag gggcacgttg accacgttcg gcgcccgagt gtcgcaccca
     5221 ctgtgcggat agaacggatg ctgaaaggtc gaacagaaca gaactcgctc atcgccgccg
     5281 aagatgtcct ccgttccatt gccatgatgc acatcgaaat ctgcgatcag cactcgctcg
     5341 agcccgtgcg ccgccagtgc gtgcgccgcc ccgaccgcca cgttgttgaa gaaacaaaac
     5401 cccatcgcgc gcgcgcgttc ggcgtggtgt cccggcgggc gaaccgcgca gaacgctcgc
     5461 tgcgcgtccc ccgccatgac caggtcgacc gcctgcacgg cagcgcccga ggcatgcagc
     5521 gcggcctcca aagtgtaggg attcatcgcc gtgtcaccat cgagccaagt cagaccctca
     5581 cgcggcgaag caccgaaaac ccgatcgatg tacgcggcat cgtgcacgcg aagcagatgc
     5641 tcgcgctcgg cccgcggtgc gtcgtagtgg tgcagcgcca tattcaagcc cgagctgagc
     5701 aaacgatcgt tgattgctcc gagccgtgcc ggctgttcgg gatggtgcgc gcccatctcg
     5761 tggagcatga aggagggatg cgtgatgtag gcgatggaca cgattttagt tccagggatg
     5821 cagcttggct gcaagcgcaa ccacacagag cctactgcat tgcagcattt cgtggatccg
     5881 ctaagagttc gactacagcc ggcgtgcgga cctcagcatt gcgctcgggt tctcgctccg
     5941 cagcaagatg aaaaagcact acctcctcaa ggtcttcgaa cccacctctg tcgccgtcat
     6001 cggcgcctcg gatcgggaga gctcggtcgg cggccagctc cttcgaaaca tccgcgaagg
     6061 cgggttcact ggcgacatct atgcggtcaa ccccaagcgc actacggtgc agggcctgaa
     6121 gagccatgcg tcgttgagcg ccatcgatcg cccggtcgac ctggccgtca tcgccatccc
     6181 cgccaagaac attccggacg tcatgcgcga atgcggcaat ctcggggttg gtgcggccat
     6241 cgtcctgtcc gcaggctttg cggaagtcgg caaggcggga acggaccttc agagtgaaat
     6301 cgtcgacatc gcacgcaccc acaacatcgc cctggtcggc ccgaactgcc tgggcatcgt
     6361 gcgcccgcgc gttgggctca acgccacgtt tgccaagagc ccggcacggc aagggcacgt
     6421 cgccctagtc gcccagtcgg gcgccttctg cacggcgctg ctcgattggg cggataccaa
     6481 gggctttgga ttttccgccg tcgcatcact cggggcgacc gcggacgtcg gcttcggcga
     6541 ggtgctcgac tatctcgcgg tggacccgga gacgcggagc atcctgctct acgtcgaggg
     6601 cgtcaccaac gcccgaagtt tcatgagcgg tctgcgcgcg gcggcccgac tcaagcccgt
     6661 gatcgtggtg aagtccggac gcagcgacag cggtacgcgc gcggcggttt ctcacaccgg
     6721 cgccctggcc ggcgccgacc gcgtgttcga tgccgctctg cggcgggccg gcgcggtacg
     6781 ggtcagctcc gtgagcgagc tcttcgccgc cgcccaactg cttgcctccg gcgcgcgggt
     6841 cgcagggccg cgtctggcga ttctcaccaa cggcggcgga cccggcgtga tggccgccga
     6901 ccgagccgct gatctcgacg ttccccttgc ggagctgagc gtggcgacgg tcgagcaact
     6961 cggtaaggtg ctgccggcac actggtcgca cagcgatccg gtcgacatcc tgggcgacgc
     7021 cgatcccgag cggtaccgcg cagctaccga aatcgtcttg gccgacccga gcgtcgacgg
     7081 cctgctggta ctgctcacgc cgcagggcat gaccgatccc acgggctgtg cggatgcggt
     7141 tatcgactcg tctcgggaat cgaccaagcc ggtgttggct tgctggatgg gcgcgggctt
     7201 ggtcaacgaa gggcgccaga agttcgccga ggccgacata ccgcagttcc gagctcctga
     7261 ggccggggtg gaggcgttcg gctatctggc gtgttaccgg cgcaaccaga ccgcgctgct
     7321 gcaggcaccg ggccccatgt cccgacacaa agcgcccgac gtcgagggtg cgcgcctcat
     7381 catcgagcat gtgctcgggg agcgccggca gacgctgagc agcgcggagg ccaaggccgt
     7441 gctgcacgcc tttcagattc ccacctcgcc gagcatcaac gtcgcttccg ccgcggaggc
     7501 attggtggcg gccgaaggcg tcggcctgcc catcgccatg aagatcaatt cgccggatat
     7561 cacgcacaag tccgatgtcg gtggcgtacg gttgaacatt cgcgaacctc gctcggtacg
     7621 cacggcgttt cgcgagatga tggatagcgt tcgcgcacag cgtcccgatg cccggatcga
     7681 cggggtgacc atcgaaccca tggtcgaccg tccgaatgct cgcgaaatca tgatcggcat
     7741 cgccaccgat ccgatcttcg gcccggtgat cagcttcggc gccggcggca ccgccgtgga
     7801 gatcttcgcc gacagcaacg tcgcactgcc gccgctcaac gagtacctca gcagggagct
     7861 catcaaaggc acccgagcgg cacgttttct gaagcatttc cgaaacctgc cggaggccaa
     7921 tgaggagaag ctgatcgacg taatgcagcg cgtctcggaa atcgcgtgtg agctgcgcga
     7981 ggtgcgggag ctcgacatca accctctgct ggtcgacgag gacggcgtca tcgcagtcga
     8041 cgcgcggatc gtcgtcgctg cctcggtcgc tagcaccgcc cattacggcc acatggccat
     8101 tcacccgtat cccgcagagc tggttacacg gtggcagcta tcggacggaa acgacgtggt
     8161 gatacgcccg attcgacccg aagacgcgac catggaacag gctttcgtac agtcgctatc
     8221 ggcggagagc aagtacttcc gcttcatgca aaacctcgac aagctgacgc cggagatgtt
     8281 ggcgcgcttc acccagatcg actacgaccg agaaatggcc ctcgtcggcg tgctggaaga
     8341 cggatcatcc cgcgcccaga tgattggcgt cgcgcgttac gtgtccaacc ccgatcgtca
     8401 ttcctgcgaa ttcgggttga cggtaggcga tgagtaccag cgtcagggaa tcggccgcga
     8461 gctcatgcgt cggctcatgg ccgtggcgcg cgaccgcggc atcgatgtca tggagggcga
     8521 ggtcctctcc aagaacacca agatgctcac cctttgcgaa cgactgggct tccgtgttgc
     8581 gcgagacccc gaagacccgg acctcgttca agtccgccgc cacctctagc gtccaaaggg
     8641 gatacgcaca tcccaagaag acgccttgat ttcagatcca gtcgggccgt tggcttcctc
     8701 ctccaaccgg ggctattcgc cgggctgtta tgctccgccc cgtgggcact ctcgaagggc
     8761 tggactgggt cgtcgtcgcc ctctacttca tcgcggtttt cggcgtcgcg gcgtgggcga
     8821 cggtttcgga gcggcgggag cagacgagct cggcagacta cttcctcgcg ggccgcaacg
     8881 tcggctggtt cgtgatcggt gcctcgctgt tcgcctcgaa catcggctcc gagcatctgg
     8941 tggggctcgc cggtaccggc gccgcgagcg gagtcgcggt agggcagttc gagatcctcg
     9001 cgtccctcat cttgctggtg ctggggtggg tcttcgtccc gttctaccta cggagcggcg
     9061 tcttcacgat gcccgagttc ctcgagcgcc ggtactcacc agcggcgcgc tggtacctcg
     9121 cggtgatctc gatcgtcggc tacgtgctca ccaagatctc cgtgaccatc gcggcggggg
     9181 gaatcgtctt cgagtccctg atgggcatcg acttctggac cggcgccctc gtcgtcgtcg
     9241 tcgcgacggg gatctacacg gtattgggcg ggctccgggc ggtcctctac acggacctgc
     9301 tgcaaatgtt cgtgctggtt ggcggcgcgg tcgcggtcac ttggatcggg ctcgacgagc
     9361 tcgggggctg gggcgcgatg gtcgaagggg ccggacccgg cttcatgtcc gtttggaagc
     9421 cgctctcgga tccggacttc ccgtggacag gaatcctcct aggcgcgccg atcctgggcg
     9481 tctggtactg gtgcaccgac cagttcatcg ttcagcgcgt gctgtccgcg gcggacgagg
     9541 acaacgctcg gcgggggacg atcttcggcg ggctgctcaa gctcttgccg ctcttcatct
     9601 tcgtcgtgcc tggtgtcgtc gcccacgcgc tcgcgtcccg cggggagatc accctcgaca
     9661 gcccggacca agcgctcccc atcctgatcg gcgcgctgct gccgcggggc atccggggcc
     9721 tcgtggtcgc aggcctgctc gcagcgctga tgagctcgct ctcgtcggtc ttcaattcct
     9781 gctccaccct gttcacccgg gacatctacc tcaagctccg acccgacgcg tcggaggcgc
     9841 gacaggtgcg cgtgggtcag acggcgaccg ccgcgctcgt cgcgcttggc ctgctctgga
     9901 tcccgatgat gcagttcatt tccgggcagc tctaccagta cctccagagc gtccaggcct
     9961 acatcgcgcc gcctatcgcc gccgtgttcc tcatcggcat cctctggcgc cgggcgaacg
    10021 cccccggtgc catcgcgtcc ctcgccaccg gattcgtctt cgggaccctg cgcctcatcg
    10081 ccgagctcac caaggaagag cacgagggat tcatcctggc gtacgcggag atcaactttc
    10141 tccacttcgc ggctcttctc ttcctggtct gcagcgtggt gctggtcggc gtctccctgc
    10201 tgacccgggc gccggacgcc agcaagctgg acggtctcac cttcgccacc accgcgccgt
    10261 cggaaaatgc ccagccttcg cgatggcgga cgcaggatcg atgggtctcg gtgctcctcg
    10321 tcgtgctggt cggcttggtc tggttctatt tcagcccgtg ggtgcaggct cgatgaccac
    10381 tgtctgaagc gatgcggtgg ggcgcgttga ccggggtgag tgcccagata tccaccccgg
    10441 cctttcggta cgcctggagg taggcgacga agtagcgcgc gaaggtgtcg tagtggccct
    10501 cgagaagccg acccccgcgg cgttgccccg gatcgtagta cttgccgttg tccttcatcc
    10561 agggcggcgc ggtccagggg gacgccacga tccgaaacga agccccgggc actgcctgcg
    10621 cgtcgatgat cagcttgaga agaccgttct gccggtcctc ttagatggag aatccggcga
    10681 ggctcggttc gtcgtcgggg gcgtagtcgt agctatactc agagaagtcc gagctcccga
    10741 catggctccg gtctcccgca tcgcatcagc ggcgcggcgc cgagctcggg cgcggtttct
    10801 ctcagagtag caacgtgagt gcggagtagg gttccgaacg cttgtggtaa aattcgcgat
    10861 ctggtactgc tggcgccacg cgaacgcttg acatgttgag agctaacaga agagaaggag
    10921 gcgtgatgac ccccaatggt ttgcgggttt ggctcattcc cctggtgacg atttccttgg
    10981 cctcggtgct cggcgtcact ccagaggcgg cagcgcaaga cggcgacgcg acccccgctg
    11041 cgcagcccct cggagccacc gaccaaacct ggaatccgga ggaaatggaa gcgctcttgg
    11101 cgccgattgc gctctatcca gacccggtgc tgacgcaggt gctcgtctca tcgaccaacc
    11161 cacaagaggt catggacgcg ggtaactggc tcgtcgccaa tccggagctc gaaggcgacg
    11221 cgctcgatgc tgctgccgag gctgcgggtt tcacggagcc gatgcgagca gtcatgccgt
    11281 tccggcagat cgtcgaccag atgtgtctcg agatggggtg gacgaccgag ttgggtcagg
    11341 cgtacaccaa cgatcagccc ggagtcctgg ccgctgtcca gcggttgcgc gcccaagcgc
    11401 aagacgtggg caaccttcag agctcggagc agatgaaggt cgagaccaag agccaggagg
    11461 gccaacaagt aatcgtgatc gagtcgccga gcccccagat catctacgtg ccacagtacg
    11521 atccggtgac cgtgtatgcg ccgccgccga cgaccgtcgt cgtcgaagac aaagggccga
    11581 gcgccggggc catggtcgcc accagcatgc tgtcgttcgg ggtaggcctc gcgatcggcg
    11641 cgtccacacg accctactac agccgaggct actgctaccc aaactactat cgcggcttcg
    11701 ttccgcctcc acccccctac tactaccgac ctgtgtatgg tggcggctac cacccgagcc
    11761 accactacaa tcgttcgtcg aactacaaca aagtgctgag cgacaacaac gtcgtcgtcg
    11821 tcaacaagaa caaggactac tggggcggct ttgatgacaa gccgcggggc aaggggcgga
    11881 cggacaatgt gaaaagtccg atcaccaagg cccgatccga ccgacccgag ctcaaagagc
    11941 tcgaacgaaa ctctggtaag aagtcagact ggaaaggtaa gtcgagctac gcgggagctg
    12001 atccgaaagc ccggtcgaag gccaagcttc ccgacaaggg caatatgccg gagtcgaagg
    12061 cagtcgctaa acaggcgaag gccaacgcgt ccgctgccaa ggcgaggaaa ccgtcgtccg
    12121 gcggcgaccg cggctacgct ggaaaggcca agccaggcgc cgcctcccgt cccgaatccg
    12181 gctccaagag gagcgccgcc ggaccgtcat cgcgccccca gcgctcgccg tcttcgagcg
    12241 gtcagggaaa gctctcgggt cgcggcggcg gagcttcgag tcgcactgca agcgcgcgcg
    12301 ggcgctccag catgccgcag ggcgcgcgaa ccaagggcgg cggcgcagca gccaacaggc
    12361 ggcggtagga gctcacgatg gagaggacat tcgcaggcat gcagaagcta ccccacagga
    12421 cgtcgatctt gttgttcgca ctcgcacttg cgagctgcac acagaaggct gaagtcgaac
    12481 cgcaagccca accgaaggac gagagtcaca cctccttccc ctcggccgaa tccgctgtcg
    12541 aggggctggt cgccgccgtc gaggcgcacg acgtggccgc gctcgagaag atctttgggc
    12601 ccggtggtga gtcgctggtg cagtggggcg atcctgtcgc cgaaaatcag gaccgagcgc
    12661 gcttcctcga gatgtatcga gcgaagcacg agctcgtgga cggccgagac ggggagaagg
    12721 tgctcgaagt cggcgccgac gactggcctc tgcctgcgcc actcgtagag aaggatggcc
    12781 actggtcctt caatggagtc gaaggtgtcg acgagctcgt ctacaggcgt gttggccgga
    12841 acgagctcgg cgcgattcgg gtcgcccacg gattcgtcga cgcccaatac gaatacgcgt
    12901 ccaagaggcg cgacggaaaa atagccggac tcttcgcccc ttacctcatc agcgacccag
    12961 ggctacacaa cgggctctac tggcccaccg agagggatga ggcaccgagc ccggccggcc
    13021 cattcgtagc ggcagccgcg gccgaggggt accggaaggc cgcggccggc gagccgacgc
    13081 cgtaccacgg ctatttctac cgcatgttgt acgcgcaagg agccgatgca cccggtggcg
    13141 ctatggacta cttcgaagac gggcagatcg tacgcggctt cgcgttgctc gcctggccct
    13201 ccgagtatgg cgtgagcggc gtcaagacgt tcatcgtcaa tcaagacgac atagtgtacg
    13261 aaaaagacct tggcgacgac accgccacgg ccgtcaacga tatcaggatc ttcaacccgg
    13321 acgagacttg ggatcaggtc cgggttgagg ctgaccagta ggcaactgct tttccatcct
    13381 gagcagcgag agagagatct cgagacgcat cctttccgtt tcccagtctg gtctcgcttc
    13441 cgtcgacgat tgcacgactt tcgctgcgac cgctgttgtc gggcaagccc gcgcaccccc
    13501 aatagcggaa gccaaaacga gccacgcagt gatttcgaga aatgtcgctc tcttgagcat
    13561 gtcgcgctgg gcgtcgcgct cgaaggcgtt tgtttttgcg aggctcggcg gggcgtttcg
    13621 cgtcttggct actgcgtcat catgatgcgc ttggcttcgg cttcgaggtc aatgtagggc
//
LOCUS       DASRZF010000394         5235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4768577, whole genome shotgun sequence.
ACCESSION   DASRZF010000394 DASRZF010000000
VERSION     DASRZF010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 5235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5235
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4768577"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..935
                     /locus_tag="VFG22_17365"
     CDS             165..935
                     /locus_tag="VFG22_17365"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET6418068.1"
                     /translation="MSGLNVTHEGGITRVVLDRPSVLNALSPGLLAELIELCADLARR
                     DSVRVVVLEGAGKCFSAGADLPAFFPLLAGPDARSTADLGRRATNAIAELPQITIAGV
                     RGHCVGGGLVLAGACDIRIASDDARFAIPELDAGIPLGWGGMEHLVRLVGTTLAADLV
                     LSCRTFGPEEALRGGLISRIVPVAALDDAISELAAAIARKPLSVLRITKRQLTEIRDG
                     EFDPGKDADALLTALKDPEVLDAGRRYVATRLRKPTDA"
     gene            984..1733
                     /locus_tag="VFG22_17370"
     CDS             984..1733
                     /locus_tag="VFG22_17370"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HET6418069.1"
                     /translation="MTSPAQNVTTISHRIRGIELDAEGSVSPGWLSRILEQTRWEVFA
                     MDDFVLRRRMEGGVARAGVFEYRVPLIFQDEVEILTWVARAGRTSLDFGHRVVRLRDG
                     AVAVRARMTVVNLGPSGPAPIDPMVASFVVDEAAPPVRPWPEGPRLHPWKRQWLVRPS
                     DQDSFRHVNQARYVDYIDDTRWFATEAGAPAGMEGRLGGLSVEYMRETHAGEQVQMET
                     WVTGDRSRAYELTRQPDGEVLARGQAFSQEH"
     gene            1775..2938
                     /locus_tag="VFG22_17375"
     CDS             1775..2938
                     /locus_tag="VFG22_17375"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET6418070.1"
                     /translation="MFPSPGGAHPPPEELFAGRYAVDGALPWGGLASYYRASSEGTPL
                     ILSVIPMDVSQSRRAEAAFAHLAHGLGSIRSRALPRILDAGVIDGVPYLAFNDTRGTL
                     LSDLLRARQLSSLTVLRIASDVLDALGEGHSHALMHGDLTPENIIVTRNRSGKLGARV
                     IGMGVSPLIRANPEASAHAAHTGSGKHAVAYMAPELFGGGSFGASADLYSVGALLHHM
                     VIGSPPIGWETGEGFDDIPGLPDVIRRAMARRPEQRFPHAAAMRAALEWLEVESAKQN
                     PHTQDIAPWMETSHVGSVPVASLASSLPPAYLGSSHPPGKILSTSGARPIPIEPIIIE
                     ALDDENKRHWSRIALLLVLLGTLVFSGYWWKSQTRPEEPAVPLGLELSAEHAE"
     gene            2928..3497
                     /locus_tag="VFG22_17380"
     CDS             2928..3497
                     /locus_tag="VFG22_17380"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET6418071.1"
                     /translation="MPNSERGSKASDEELMEAYVNGDDAAFRALFERYAPILLRLTRR
                     HLRNDELAEEIVQQTFFRLHGARKDFRPGSKLRPWIMTIAMNLVREHWRRTKRRKMTS
                     IEVDTQAAPEADLMPLELRQRSELLHVALEKLPTSQREVVELHWFQERPYAEVAQIIG
                     TSEGAVRVRAHRAYATLKQLLVLEIRGDE"
     gene            3494..4216
                     /locus_tag="VFG22_17385"
     CDS             3494..4216
                     /locus_tag="VFG22_17385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418072.1"
                     /translation="MTSVDNRPEDDLAERLRELDVADVDARSERMEHLFTGIKAECQK
                     SDRTVAGFLRSRSTMARRLIVLGVFSALAITGWYLFPIVGDHARTIQWTTSLVTFCVL
                     LLAAMIMVTRPVHLPELPRWQSVCLVCVALGATVLAAFWPYPAAQAATHGHDGSVMAG
                     ACMGVGILLGVPVYALLRLVDRGNAFGSLVAAAAAGLAGNFVLKAHCSVPGTSHELLG
                     HASVAVLFVLGLGLVHRWMPSH"
     gene            4330..>5235
                     /locus_tag="VFG22_17390"
     CDS             4330..>5235
                     /locus_tag="VFG22_17390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HET6418073.1"
                     /translation="MSEELRREFNEKGIRKVKVGGFDVDGVLRGKYISLEKFWSALDK
                     GFGFCDVIFGWDVSDRLYDNARVTGWATGYPDALARIDPSTFRVLPDAPDTANFLVDF
                     YTPDGAPHPACPRNLLKKVRQRAHAAGFSATFSAEFEFWVFQETPQSLHQKDFRNLEP
                     LSPGMFGYSWLRAGQHQFLVDDILETSAEYDIEIEGLHTETGPGVWEAALRYDDVLAA
                     ADKAALFKTTLKQICARHDLAVTFMAKWNADLPGSSGHLHQSLWDAGGQINLFAAAGS
                     DKLSKTGLHYLGGLVTLAPELTALYS"
BASE COUNT          926 a         1658 c         1666 g          985 t
ORIGIN      
        1 tgggtcgcgg gctgatcttc gatcctgact tgccgaagcg cgcccagtcc gatgccaact
       61 acgagaacgg ctgcaatcac tgcaaccaat gcgcgacgct gatcgaagcg ccgggcggga
      121 tctactgcgt cgagcggccc gccaactttg cttgaggcca ggtcatgtcg ggattgaacg
      181 tcacgcacga aggagggatc acgcgagtcg tgctcgatcg gccgagcgtt ctcaacgcgc
      241 tctcgccggg cctcttggct gagctcatcg agctctgcgc ggatctcgca aggcgcgatt
      301 cagtccgtgt cgtggtgctc gagggcgcag gaaagtgctt ctccgcaggg gccgacttgc
      361 ccgcattttt ccctctgctg gccggcccgg atgcgcggtc gacagccgac ttggggcgcc
      421 gcgcgaccaa tgcgatcgcc gagctgcctc agatcacgat cgccggtgtt cggggacatt
      481 gtgtaggagg cggattggtc ctcgcaggtg cttgcgatat tcggatcgcg agcgacgacg
      541 cccgattcgc cattccagag ctggatgccg gcatcccgct cggatgggga ggcatggagc
      601 acctggttcg actcgtcggc acgactctcg ctgctgatct ggtgctgagc tgtcgcacgt
      661 ttgggcccga ggaggcgctt cgtgggggct tgatcagcag aatcgtacca gtggcagctt
      721 tggacgacgc gatttccgag cttgccgcag ccatcgcgcg caagccgctc tctgtgcttc
      781 gcatcaccaa gcgtcaactc acggaaattc gtgacggcga attcgatcca ggcaaagacg
      841 cagacgccct cctcaccgca ctgaaggatc cagaagtgct ggacgcgggc cggcggtacg
      901 tcgcaacgcg tctccgaaag ccgacagacg cctgacgagc atcctgcccg tccccgcgag
      961 tcctgctagg ctcgccgccg cacatgacta gcccagctca gaacgtcacg accatcagcc
     1021 ataggattcg aggcatcgag ctcgacgccg agggttccgt gtcgcccgga tggctctccc
     1081 gcattctcga gcaaacccgc tgggaggtct ttgcaatgga cgacttcgtg ctccgcaggc
     1141 gcatggaggg tggcgtcgcg cgtgccgggg tattcgaata ccgcgtacct ctgatctttc
     1201 aggacgaagt ggagatcctg acttgggtcg cccgggccgg ccgcacgagc ttggacttcg
     1261 ggcatcgggt ggtccgcctg cgcgatgggg ccgtcgccgt gcgcgcccgc atgacggtcg
     1321 tcaacctcgg acccagcggc ccggctccta tcgaccccat ggtggcgagc ttcgtggtcg
     1381 atgaggccgc gccgcccgtt cggccctggc cggagggacc gcgcctgcac ccgtggaagc
     1441 ggcaatggct cgtccggccg agcgaccaag acagtttcag gcacgtcaat caggcgcgct
     1501 acgtcgacta catcgacgac acgcgctggt ttgcgaccga agcaggagcg cctgccggga
     1561 tggaaggacg tctaggggga ttgtcggtgg agtacatgcg ggaaacgcac gctggtgagc
     1621 aggttcagat ggaaacctgg gtgacggggg atcgtagccg cgcgtacgag ctcacccggc
     1681 aaccggatgg cgaagtgctg gctcgaggcc aggccttttc acaggaacac taaagattcg
     1741 cgccccggga gcaatcggtc tattctcgga ctcgatgttt ccctcgcctg gtggcgcgca
     1801 ccctccgcca gaggagctct tcgccggccg ctacgcggtg gacggcgcat tgccgtgggg
     1861 aggtctcgcc tcttactacc gcgcatcctc ggaggggact ccgctcatac tgagcgtgat
     1921 ccccatggac gtgagccaat ccaggcgtgc cgaagccgcg ttcgcacact tggcacatgg
     1981 cctcggttcg atccgttcgc gcgctcttcc gcgaatcttg gacgctggcg tgatcgacgg
     2041 cgtgccgtat ctggccttca acgacactcg cggcacgctt ctctccgacc tccttcgagc
     2101 tcgccagctg agctcgttga ccgtgcttcg gatcgcaagc gacgtgctcg acgccctcgg
     2161 tgaagggcac tcccacgcat tgatgcatgg cgacctcacg cccgaaaaca tcatcgtcac
     2221 tcgaaatcga agcggaaagc tcggcgcacg cgtcatcggc atgggtgttt cgccattgat
     2281 tcgagccaac ccggaggcga gcgcccacgc tgcacataca gggtccggaa agcacgcggt
     2341 cgcatacatg gcccccgagc tcttcggcgg ggggagcttc ggagcctccg ccgacctcta
     2401 ctccgtgggc gcgctgctac accacatggt gatcggatct cccccgatcg gctgggaaac
     2461 cggggaaggt tttgacgaca tccccggtct gcccgatgtc atcaggcgcg cgatggcaag
     2521 acgtcctgag caacgattcc ctcatgccgc tgccatgcgg gctgccctcg agtggctcga
     2581 agtcgagtct gccaaacaga acccgcacac tcaggacatc gcaccctgga tggaaacctc
     2641 gcacgtcggc tcggtgcccg ttgcgtcgct cgcctcgagc ctgccgccgg cgtacctcgg
     2701 cagtagtcac ccgcctggaa agatcctcag cacctcaggc gcgcgaccca ttccgatcga
     2761 gccgatcata atcgaagcgc tcgatgacga gaacaagcga cactggtcgc gaatcgctct
     2821 tctactggtc ttgctgggca ccctcgtgtt ctcggggtat tggtggaagt ctcagacccg
     2881 tccagaggag ccagccgtgc ccttgggttt ggagctgagc gcagaacatg ccgaatagcg
     2941 aacgaggctc gaaggccagc gacgaagagc tgatggaagc ctacgtcaac ggggatgatg
     3001 cagcatttcg tgctctgttc gaaaggtacg cacccattct tctccgcctc acgcggcgcc
     3061 accttcgcaa cgacgagctc gccgaggaaa tcgtgcagca gaccttcttc cgtcttcacg
     3121 gtgcgcgaaa agacttccga cctggttcga agcttcgccc ctggatcatg acgatcgcga
     3181 tgaacctagt ccgcgaacat tggcgccgga cgaagcgtcg aaagatgacg agcatcgagg
     3241 tcgacactca ggccgcgccc gaggcggacc tcatgccgct cgagcttcgc caacgctcgg
     3301 agctgctcca cgtggcgctc gagaagctcc cgacgagcca gcgcgaggtg gtggagctcc
     3361 actggttcca ggagcgccca tacgcggagg tagcccagat cattgggact tccgaggggg
     3421 ctgtgcgtgt tcgcgcacat cgtgcgtatg cgactctgaa acaattactg gtcttggaaa
     3481 tacgtggtga tgaatgacta gtgtcgacaa caggccagag gacgacctgg ccgagcgctt
     3541 acgcgagctc gatgtggcgg acgtggacgc ccggagcgag cggatggagc acctgttcac
     3601 cgggattaag gcagagtgcc agaagagcga tcggacggtg gcaggctttc ttcgaagccg
     3661 gtccacgatg gcacgccggc tgatcgtcct cggtgtattc tcagcgctcg ctatcacggg
     3721 ctggtatcta tttccgatcg ttggcgatca tgctcgcacg attcagtgga cgaccagtct
     3781 cgtgacgttc tgcgttctgc tactcgcggc gatgatcatg gtgacccgcc cggtccattt
     3841 gcccgagctt ccgcgttggc agtcggtctg cttggtttgc gtagcgctcg gcgcgacggt
     3901 tctggccgcg ttctggccct atcccgcggc gcaggccgcc acgcatggtc atgacggcag
     3961 cgtgatggcg ggcgcctgca tgggggtcgg catcctcctc ggcgtcccgg tctacgcctt
     4021 gctcaggctc gtcgatcgag gcaacgcgtt cgggagcctc gtcgcggccg cggcggccgg
     4081 cctcgcgggg aacttcgtgc tgaaggcgca ctgctcggtt ccgggcacct cgcacgagct
     4141 gcttgggcac gcatcggtag ccgtgctgtt cgtgttgggt ctgggcctgg ttcatcgttg
     4201 gatgccttcg cactagcgag cgattctcgc tgagcggttt cgtcgccgat gagccgcgtt
     4261 ggctgggccg ggatcgctcc gcacaagagg gcgaaaaagg agtatgcttc cggccgccga
     4321 ggaggaattg tgagcgaaga gctgcggcgt gagttcaacg agaagggaat ccgaaaggtc
     4381 aaagtcggag gtttcgacgt cgacggagtg ctccgaggca aatacatcag cctcgagaaa
     4441 ttctggtcgg cgctcgataa gggcttcggt ttttgcgacg tgatcttcgg atgggatgtc
     4501 tcggaccgcc tctacgacaa cgcgcgagtg acaggctggg cgaccggcta tccggacgcc
     4561 ctagcccgaa ttgaccccag cacgttccgc gtcctcccag acgctccaga cacggcgaac
     4621 ttcctcgtcg acttctacac gccggatggc gcgccgcatc cggcatgtcc ccgcaacctg
     4681 ctcaagaagg ttcggcagcg cgctcatgcc gctgggttct cggcgacttt ctcggccgag
     4741 ttcgagttct gggttttcca agagaccccc caatcgcttc atcagaagga cttccgaaac
     4801 ctcgagcctt tgagcccggg catgttcggc tactcctggc tgcgtgcggg ccaacatcaa
     4861 ttcttggtcg acgacatctt ggagacctcg gccgaatacg acatcgagat cgaagggctc
     4921 cacaccgaga cggggcccgg cgtttgggaa gcagcactgc gctatgatga cgtgctcgcc
     4981 gctgccgaca aggccgccct cttcaagacg accctgaagc aaatctgcgc tcgccacgac
     5041 ctggcggtta cgttcatggc gaagtggaac gccgacctcc ctggttcgag cggccacctc
     5101 catcagagcc tttgggatgc cggtggccag atcaacctct ttgcggccgc tggtagcgac
     5161 aagctgagca agaccggcct tcactacctg ggcggtctgg tgacgctggc cccagagctg
     5221 acagcgctct actcc
//
LOCUS       DASRZF010000395        39585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2709221, whole genome shotgun sequence.
ACCESSION   DASRZF010000395 DASRZF010000000
VERSION     DASRZF010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 39585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39585
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2709221"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="VFG22_17395"
     CDS             complement(<1..800)
                     /locus_tag="VFG22_17395"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HET6418074.1"
                     /translation="MRPEWKTGMTLEQLQSRGVVGRIRGSVSQRVIGVQHDSRCVEPG
                     DLFVAVPGATHDGSSFVRDALRSGAVALMAERELASDVPLMIVADARLGLGQAAELVY
                     GEPTSALPTVGITGTNGKTTTAYLLKQAIEGASGKPALVGTTGLIGLGDTTPSLHTTP
                     EGDDISRFARIALDQGATHLVLEVSSHGLAMRRVDALDFAVAAFTNLSRDHLDFHGSM
                     ERYGEAKARFFTELAPKHAVIHVDDAFGAELAERIPVPVTRCSRRPES"
     gene            complement(790..2844)
                     /locus_tag="VFG22_17400"
     CDS             complement(790..2844)
                     /locus_tag="VFG22_17400"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HET6418075.1"
                     /translation="MKNRRQRWFRARIALLGIGVMGVAMIVMVRAFHVQIASGDRLRG
                     MAESQHVRELRVSPRRGVIYDRHGAELAVSADVDSVYANPKRLKAMEQDPRTVARRIA
                     QILDVDAQKLAGRLASDRYFVWIERQVTPHEATRIRELDIPGVELTTEARRYYPNRHL
                     AAHLLGFADIDGRGIEGVELAYEDRLRGSDRRVEAIRDRRGQVVFADEMQDDRSMQGQ
                     NVVLTIDKAIQHIAERELALGVRTFEARGGSVVVMDPSTGEILALANYPPFNPNEPSK
                     HPTAHRRNRAVVDRFEPGSTVKPFTMAAALAAGTVKPNQSINCENGVTRLGGRLLHDA
                     HPYEWLTPTQVLAYSSNIGTAKIALDLGKKELYRGLRRFGFGEPTGLGVPGETAGILR
                     HYRRWYEIDTATVSFGQGMSVTNVQLATAMSAIANGGQLMQPLLVRRMTDGHGATIEE
                     NKPRVRRQVVPRRVAKLVGQMLTAVTEPGGTAVEAAVDGYLVAGKTGTAQKADYVHGG
                     YAKDQWLASFIGFAPADRPAIVISVVIDEPAIAHYGGTVAGPVFRRIAEVTLRHLGIA
                     PEGRQAVLAKKHQERIAISEVEPAAPDEPVEKGESAVPDVRSLPLRQAVMALHAQSLV
                     PEVQGSGLVVAQDPAPGKAVAHGAVVRLRLDRQRFDTEAATSGGSTNKLAAAAPRRPH
                     AP"
     gene            complement(2841..3146)
                     /locus_tag="VFG22_17405"
     CDS             complement(2841..3146)
                     /locus_tag="VFG22_17405"
                     /inference="COORDINATES: protein motif:HMM:NF016860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HET6418076.1"
                     /translation="MKGRFLPLWCVAVFATAAAFVVHLSIRFETVRLGYEVSEERGRQ
                     SELLQDRRLLSIEAATLRQSGRIETIARGTLGMEVPKPTRIVTMKEQKTRSAAGRVR"
     gene            complement(3143..4033)
                     /gene="rsmH"
                     /locus_tag="VFG22_17410"
     CDS             complement(3143..4033)
                     /gene="rsmH"
                     /locus_tag="VFG22_17410"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HET6418077.1"
                     /translation="MTGFEHTPVLLAELLEQLAPAPGGTYADVTLGGGGHARAILERS
                     APDGRLIGTDRDPSALDAARAELAEFGQRVTLRKARIRDLRDVLTSLDIEQVDGIVAD
                     LGVSSAQLDQAERGFSLANDGPIDMRMDPTEGNSALDLIAYSDAEELANLIYRYGEEH
                     RSRKIARALRHAYEEGELETTGDLRRAVHRATGPRRGRIDPSTKTFQALRIAVNDELG
                     ELESLLEQLPSVLRPGGVIAVISFHSLEDRIVKHGFRESEHLVPLTKRPIIASESERE
                     ANPRSRSAKLRAARRLEVAA"
     gene            complement(4030..4482)
                     /gene="mraZ"
                     /locus_tag="VFG22_17415"
     CDS             complement(4030..4482)
                     /gene="mraZ"
                     /locus_tag="VFG22_17415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HET6418078.1"
                     /translation="MFRGRYEHSIDAKGRTSVPSRFREVMTSQGDAKLVVTTGLDTCL
                     VAYPMAEWLAFEKRLSELPQFDTDVVTLKRIYVSGAVECDVDRVGRLLIPSALRKHAD
                     LKRDALWAGMGSYIELWAKASFEDLRKDVLADEDRRLAIAKRLAELGL"
     gene            4781..5125
                     /locus_tag="VFG22_17420"
     CDS             4781..5125
                     /locus_tag="VFG22_17420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418079.1"
                     /translation="MLFSALVDPIDEAWAQVEAGWGDEEAHRRFVGVCVALDRLPEAG
                     KRYRMVRETDPMRSEAASKQIDRLIGLATQQLQDTRVAPSGVAHKRTLTWAAFFIMLV
                     LMGAGVLFLLRA"
     gene            5158..5832
                     /locus_tag="VFG22_17425"
     CDS             5158..5832
                     /locus_tag="VFG22_17425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HET6418080.1"
                     /translation="MQVDFAAKELTVKLVYYGPALSGKTTNLRAIHWLGTSKACGELM
                     TLETQNDRTLFFDMLPITVSSESGIKVRLKLFTVPGQVMHNATRRLVLQATDGVAFIA
                     DSQRSEAAANRESFLNLKRNLAENGIDPDRVPIIIQFNKRDMDDIRTDEELEAIARQS
                     REPIYTAVATRGYGVMQTLRGLIRETWSKLEEEHGLHEKFGVSPVRFMQEVESKLKHD
                     PRQQER"
     gene            5832..7235
                     /locus_tag="VFG22_17430"
     CDS             5832..7235
                     /locus_tag="VFG22_17430"
                     /inference="COORDINATES: protein motif:HMM:NF021619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6418081.1"
                     /translation="MQDDTNAADILMGDVVRLEDVLSRDSVTSVSKSFFTLFGLPVRV
                     ISHEGDLLADVHRDRPLCNYLNTSPGGERACAQTVGAVRDLEPDGETIVHPCFTGAVY
                     RIVPLHYQGRPIGRFIIGPYVPAETKRAPASLTAVDPSIDAGAARGHFDRMPRVREDI
                     AEQLSAHLKGVLELLVFSGHRAQLASTMQVASVRENYRELAEKNEALKTSYDELKQLD
                     QLKSTFLATVSHELRTPLTSIIGYTEMLEGGAAGALNEGQSDFLETIRGKADQLLGLI
                     SSLLDLGRLEAKNLELLNEAVDPRTLLSDIGSTIVPNANRRNVALEIRVASGVPKIWG
                     DPVRLRQILLNLADNAVKFTDAGGEVELSAEAGELGGGTSSGLGAALFAASRPAVILT
                     VRDTGMGIPEDKLPRIFDAFYQIDAGTTRTHGGAGLGLSIVKQLVNGHDGKIEVTSSP
                     GEGTIFTVTLPAAHDDD"
     gene            7228..8178
                     /gene="thiL"
                     /locus_tag="VFG22_17435"
     CDS             7228..8178
                     /gene="thiL"
                     /locus_tag="VFG22_17435"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HET6418082.1"
                     /translation="MIDEFAKIEWLKARFELDGDSNGILVGIGDDAAVIDFGDRPSIV
                     TVDAHVENVHFRRDLISCRELGYRALVAAVSDVWAMAAVPRAALIALTLPPSFSDGDF
                     RELIEGIAEAATGTGAKVIGGNLSASESLTVTTTALGTPLGKPLMRAGARPGDAIYVT
                     GTLGAAALGLAVLESRSFGLEHGRRFVERWKRPPINGYALDVLAAVATAAVDVSDGAL
                     QDLGHLCTASGVGAVLRASSVPTATGYETSCAALDLDPTKLSLTGGEDYELIFTAPEC
                     SEAEKIGSKIGTVIEGNGTRVLDEAGEQIDLGNAGFRHFA"
     gene            complement(8166..9332)
                     /locus_tag="VFG22_17440"
     CDS             complement(8166..9332)
                     /locus_tag="VFG22_17440"
                     /inference="COORDINATES: protein motif:HMM:NF018743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HET6418083.1"
                     /translation="MRTGLDRIADGDLDAVRLVAGRNVGLLAHAASVDRRLRPAASVL
                     RAAGANVAALFGPEHGFAGAAQDMVGVGTSDQDEVPIYSLYGDDSGDLSPKPEWLRGL
                     DAIVIDLQDVGSRYYTYVWTAALTMKAAAAVGTEVVLLDRPNPLGGARVEGAPQRDGY
                     LSFVGLYPVAVRHGLTIAELMHWVCDLEKIDRSVLNIVPLDGWMRSMNWKDTGLPWVM
                     PSPNMPTPDTALVYPGGCLVEGTRLSEGRGTTRPFELWGAPGLDVRPLLELDLHGVTL
                     RPVELTPTFQKHAGLSCAGVQVHVTDPWVFRPYEAYLRMLAAVLPQLEPVERWRTDVY
                     EFVSDRPAIDLLTGGPEFRTAVDGGASLDTVLANEARGAALFDDERRSAWLYAK"
     gene            complement(9338..10291)
                     /locus_tag="VFG22_17445"
     CDS             complement(9338..10291)
                     /locus_tag="VFG22_17445"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HET6418084.1"
                     /translation="MKPFAHRSLLDIEGLTRVDVERILDTAEAFLQVSRRPVRKVPTL
                     RGKTVINLFYEASTRTRTSFELAGKRLSADVINMTVSASSVKKGETLQDTCRTLEAMH
                     PDVIVIRHSASGAASYVASKIGCSVINAGDGMHAHPTQALLDAFTIRREKGTLDNLVV
                     TIVGDIAHSRVARSNAHLLKLFGCEVRVVGPRTLLPAAVDSLGVRVFDQPAQALEGAD
                     VVMVLRIQQERLEGALLPSLREYARTYGIGPRAIARAKPDAIIMHPGPMNRGVEIESV
                     VADSERSVVLDQVEAGVAVRMALLYLFAGETSDAPAVGEAD"
     gene            complement(10288..10830)
                     /gene="pyrR"
                     /locus_tag="VFG22_17450"
     CDS             complement(10288..10830)
                     /gene="pyrR"
                     /locus_tag="VFG22_17450"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HET6418085.1"
                     /translation="MARNQKVVLDADAIQRSIRRIAVAILEATSGIEDLTLVGIRRGG
                     VALSRRIADEIAAVEGRPPPVGTVDISLYRDDAATALPNPKIGPSDIPFDVNGRHVVL
                     VDDVLHTGRTVRAAIECLQDHGRPARVWLAALCDRGGRELPIAPDFVGRKISMDPGGR
                     LDVWLDAAGPDRVVLSEGDE"
     gene            complement(10871..11641)
                     /locus_tag="VFG22_17455"
     CDS             complement(10871..11641)
                     /locus_tag="VFG22_17455"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6418086.1"
                     /translation="MISEGTVVVTGATRGIGRATVDAILARGGKVVAVARDPEALRLL
                     VASSEGRIRAVSADLEDVARVQDAAKRAIDAFGPIDGLVNCAGIARYEPVGAISLASI
                     EAQLHVNFVAPLLLSQALAEHFGTRGGSIVNVSSTLSERVAPNTLVYAASKAALNAMT
                     KTLALELARFGVRVNAVLPGGVETDMLRNPRLRPGESLAPDELDTRVAAQLKALAALH
                     PMGRLGEPREVAAVIVAVLDQTWQTGSLVTIDGGLSVA"
     gene            complement(11646..12392)
                     /locus_tag="VFG22_17460"
     CDS             complement(11646..12392)
                     /locus_tag="VFG22_17460"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HET6418087.1"
                     /translation="MDVLRIEELMVDCVVGVYPHERNASQPLRVDIEMRLQTEASAVK
                     ESLRSTIDYAATASQVVFLLRSCRFRLLETAAHVLARYILAPPAPDERRAQVDSALVR
                     LSKPGALRGFAIPSLEIERDASWCSVEVEQKPFGTVDVIYETKGAGIYRLNVAPGKSI
                     PLHVHHEMRESEMVLSNGLLCQGQACAPGTVHRWPRGAAHTYMNPSERHQNILCVDSP
                     RFIPEDEIEVTGQPADVPAEAPWGPIAGWG"
     gene            12441..13718
                     /locus_tag="VFG22_17465"
     CDS             12441..13718
                     /locus_tag="VFG22_17465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET6418088.1"
                     /translation="MSQPDSIPTNEIAEVAAFRPVPRTGVIYVTVEAQKRGFSLDAPD
                     WCNLGQGQPDTTALPGAPDRVTNIPVEPGDLDYAPVPGIWELREDVAGLYNKLFRRGM
                     KSQYSAENVAIAGGGRVAVMRACAAIAPIHLGHFLPDYTAYEELLDVFRRFLPIPILL
                     DPKQGYEFTREQLEEEILGRGLSAILASNPCNPTGKVVQGDALAGWVETARNSNCALL
                     LDEFYSHYVWNDEPTIVSAAQYVEDVDEDPIVVFDGLTKNWRYPGFRVSWIVGPKRVI
                     ESAASAGSFLDGGGVAPMQRAAIDLVQEAPTLAEAAAVHTEFKKKRDFLVKGLKDIGV
                     RFDLEPQGTFYAWGNLSGLPPSIRNSDDFFRAALDRKVICVPGHFFDVNPGKRRTGRP
                     SRFIEHVRFSFGPPMPVLEEALDRLRAMLAEAT"
     gene            13811..14677
                     /locus_tag="VFG22_17470"
     CDS             13811..14677
                     /locus_tag="VFG22_17470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HET6418089.1"
                     /translation="MTTSAAVALRLEQQPREAAPFVKWAGGKGRLLSQLRPLLPSGVE
                     HMRHVEPFVGGGALFFSRRPGRALLTDINPALVGTYVAIRDDLNGVISALRGLAKGHS
                     KEGYYQVRERYNQGRRISTAKRAAMFIYLNKTCFNGLHRVNRKGEFNVPVGSYKNPRI
                     LNEEGLQAASQALKHANLQCAPFDALLENAKPGDFVYFDPPYEPVSPTASFTSYARDG
                     FSQEDQTRLRDVYKALDRRGCKLMLSNSDVPFIRELYAEFEVETVAAPRAINCDARGR
                     GKVREVVVRNYV"
     gene            14895..15980
                     /locus_tag="VFG22_17475"
     CDS             14895..15980
                     /locus_tag="VFG22_17475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418090.1"
                     /translation="MFSIVVRQPLAKGTDSREPGLVRAVALVSVLGFSLLACSQEAES
                     DQLTPTLKHSFEPLMIAAGDEMTGVCQSWTLDNDEPLYVSKIRQRNDGAWHHSNWFFV
                     PEGTYAGPDGTWNCDDRDFHDVTAALEGGAFFAQSTQTLQEVQAFPDGAVLEIPPRHK
                     IVGGVHLVNVSASPLETALSFDIETVSEDRVEVRLRPIAFTNTALDIEPQAESRFSMT
                     CDLGKEFERFLGAPPNYNIYYVLAHYHEWGNYFRLSFVDETGGDRTIFELENSVGEPL
                     GITLDPPINSENAPKLRVECGYLNNTDERLKYGLGGKEMCVFLAYTDADLTFGATSGG
                     NNALGPNEDGIYMNETDCGIVLAVPAN"
     gene            complement(16027..16287)
                     /locus_tag="VFG22_17480"
     CDS             complement(16027..16287)
                     /locus_tag="VFG22_17480"
                     /inference="COORDINATES: protein motif:HMM:NF037605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5522 domain-containing protein"
                     /protein_id="HET6418091.1"
                     /translation="MKDRDEPPIDEQVGRALDETLTPDIVDAHRAALARGEEGYLDPR
                     TGLYVMTADTLRARRKCCGSGCRHCPYSADEQRAAGRPLIRR"
     gene            complement(16309..17463)
                     /gene="alr"
                     /locus_tag="VFG22_17485"
     CDS             complement(16309..17463)
                     /gene="alr"
                     /locus_tag="VFG22_17485"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET6418092.1"
                     /translation="MQSDFPTPLRPRVQSADGSLRPTRAEVMLDAIGHNLRVLRSAAG
                     GRKILAVVKADAYGHGVVPVARRLQRDGVDGFGVALAEEGIELREAGIDRAILVLNGI
                     SGGAHRDIIAVGLTPVLYELSEASAFDAVSDGRPIDVHLKVDTGMGRLGVPVAELSVF
                     LRELRRFPSIRIAGLMTHLSTADTDPEYVAEQLAGFARAEGLVRRFGHQPSVLHAANS
                     AAVFRHPESRFDWVRPGIALYGYPGADTVDAPLRHAMRWRTEILRLRTLQPGESAGYG
                     RSFRATRPTLLATIPVGYGDGLLRAASNRGQVLIDGVRCPIVGNIAMDLTTVDVSAIP
                     SVAIGDEVLLLGEQKGAVLDARDLAAAARTIPYEVLTNVSRRVPRIYVDG"
     gene            17540..18439
                     /locus_tag="VFG22_17490"
     CDS             17540..18439
                     /locus_tag="VFG22_17490"
                     /inference="COORDINATES: protein motif:HMM:NF016952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HET6418093.1"
                     /translation="MAGTERRMREAAKRAQHRSSGLLWAGMIAAVLIAVGVVVAPGLR
                     NAGEETVSPVDLGAAEVSPAPVLPQVEQLRVHVIRKYPHATDAFTQGLIWHGGVMYES
                     TGQYGKSELRRVRLKDGNVLARRKLDPGFFGEGLALVDQRLIQLTWRSGIAFVSDVAT
                     LQERETLRYAGEGWGLCYDGTSLVMSNGSSTLDFRDPGSMALIRKLNVTKDGLPVDEL
                     NELECVGREIYANVWRHDEIVRIDRATGRVTGSIDASGLLTRIESRRADVLNGIAYKP
                     DTKTFLLTGKLWPQVFEVDFEPR"
     gene            complement(18406..19275)
                     /locus_tag="VFG22_17495"
     CDS             complement(18406..19275)
                     /locus_tag="VFG22_17495"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HET6418094.1"
                     /translation="MRRIVQGDNLEVLPTLPRKHARLIYIDPPFNTGRQQSRQRIRVR
                     ASADGERSGFGGRRYDVERVDSPSYRDDFDAYVPFLMRRIEAALPCLTDDGSLFVHLD
                     SREVHYVKVALDGLLGRDCFFNEIIWAYDFGGRPKRRWPAKHDTLLWYVRDPARYVFQ
                     FDEMDRIPYMAPGLVGKEKAARGKTPTDVWWHTIVPTNGSEKTGYPTQKPLGVLERII
                     KVHSEPGDTILDFFAGSGTTGEAAARLGRGFVLVDEHPEAITTMRDRLASWEPDVEVR
                     GPAQRGSKSTSKT"
     gene            19363..19563
                     /gene="thiS"
                     /locus_tag="VFG22_17500"
     CDS             19363..19563
                     /gene="thiS"
                     /locus_tag="VFG22_17500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003110013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HET6418095.1"
                     /translation="MRLLVNGEEKQVQPSTTVKQLLASLGLADTLVAVERNEEVVPRA
                     RHESTTLNDGDRVEVVHFVGGG"
     gene            19564..20361
                     /locus_tag="VFG22_17505"
     CDS             19564..20361
                     /locus_tag="VFG22_17505"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HET6418096.1"
                     /translation="MSEDVLKIGQFEFRSRLFTGTGKYPDLETARNALRASACEVVTV
                     AVRRVDLKATGADSIMTVLREGNYTLLPNTAGCYTAEDAVRTARLARELLDTPLIKLE
                     VIGDERTLFPDVPATLEAAKILVDDGFTVLPYFNDDPVSARRLEDLGCAAVMPLAAPI
                     GSGLGVRNPFNIEIIVKHANVPVIVDAGVGTASDAAVAMELGIDGVLMNTAIAGAKDP
                     VRMARAMKAAVEAGRDAFLAGRIQRKLYATASSPLEGVIGSDQDGRT"
     gene            20371..21018
                     /locus_tag="VFG22_17510"
     CDS             20371..21018
                     /locus_tag="VFG22_17510"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET6418097.1"
                     /translation="MTQGLPRIWIITDPDHPDGPVAPLRRALEICPPGLVGVQLRAHG
                     VPDRQLIEWGLELRALTQAQGCTLAVNRRIDVAEIVGADGVHLPESGLPLKDVREHWP
                     AIHLLGVSRHDSVGLAAAERGGASYAYLSPVFTVPGKAAPIGIHGFRAAIADVDIPTY
                     ALGGIEAEDLNPLLAAGAFGVAIRRAIYASDSPEESLSRFLSALDKAGANGEERA"
     gene            21018..21644
                     /gene="lepB"
                     /locus_tag="VFG22_17515"
     CDS             21018..21644
                     /gene="lepB"
                     /locus_tag="VFG22_17515"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET6418098.1"
                     /translation="MGAIRTGLGCLSWVGAGLIVIALILKALFFDMAELAHNGMAPTL
                     LRGERVLIHKKGSPTLGSIAVCQHPSEEGWVVSRVAAIEGMKIDSFGKELRIDGEPVV
                     FDQHGTTSFYNADTDVTASVVWGDEHFGPKPHLIFMEEDGRHRIRETELATGEIYLLN
                     DYRAHWVQDSREFGPVDASSCRGTIVFRLFPVEGLDREIAHHYFELIR"
     gene            21714..22409
                     /gene="purQ"
                     /locus_tag="VFG22_17520"
     CDS             21714..22409
                     /gene="purQ"
                     /locus_tag="VFG22_17520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HET6418099.1"
                     /translation="MKVAVVQFPGSNADWDALHAVRDVLGADAWYAFHKEHGLGDADA
                     VVIPGGFSYGDYLRAGAIAGFSPIVSSIREFAGRGGPVLGICNGFQILTEMGLLPGAL
                     TRNAHLRFECRDIHLRVETEGPFTDMGKGTILRLPIAHADGRYHTTEDGLAELERNDQ
                     VAFRYTTPTGDPPNGSDANPNGSVSDIAGVYNREKNVLGMMPHPERASEGILGNEQGL
                     TLFTSLRRHLGFE"
     gene            22406..24688
                     /gene="purL"
                     /locus_tag="VFG22_17525"
     CDS             22406..24688
                     /gene="purL"
                     /locus_tag="VFG22_17525"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF002290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HET6418100.1"
                     /translation="MSRPANFPGEPDSSPEIAREFGLAQHEWDRILSTLSRAPSYAEL
                     GVISVMWSEHCSYKSSRVHLARLPTTGPQVVQGPGENAGAVDIGDGFCAVFKMESHNH
                     PSYIEPYQGAATGVGGILRDVFTMGARPVASLNSLRFGRPEHPKTPHLLKGVVAGIGG
                     YGNCIGVPTVGGEVQFDASYDGNILVNAFTVGIAKTDDLFFGVAEGVGNPIIYVGAKT
                     GRDGIHGATMASAEFGEETDSKLPTVQVGDPFMEKLLLEACLEIFATDCLSGVQDMGA
                     AGLTSSSVEMADRAGNGIELDLDAIPRRTKALSPYEMLLSESQERMLMVAKEGREQEV
                     FELCAKWDLDASIVGRVTDTGRFVCKATPGYDPFDGTGKPDPIVVVDLPVDLLTNAAP
                     KYDRPQQERAETASLVSMAGDAPDDLGDELVRLIGSPNIGSRSWIWRQYDHIVRDGTV
                     IRPGEGDAAVIRVFCDDADGTREKFLAISADCNGRHVELDPFAGAAMAVAECTRNVVC
                     VGAKPLGLTDCLNFGNPEVPEQMWRFARAIDGLAAACTALDAPVVSGNVSLYNETDGK
                     AILPTPTVAVVGQLEDPEHRLGLGFSNEGDLIAHLGRPSRGVLGGSEWLVQRTGSVTG
                     APVGIDLGDEAALQRAVLALARAKLLSSAHDVSDGGFAVCLAEACIAHRHGAKVSLPS
                     NEDAPAHALMFSEEPSRIIVSLSPGHLGEVEEICQRHGVPFEKLGIVTGQSLVIEGVC
                     DVSIAALEKSHSTALDAIVS"
     gene            complement(24789..28394)
                     /gene="smc"
                     /locus_tag="VFG22_17530"
     CDS             complement(24789..28394)
                     /gene="smc"
                     /locus_tag="VFG22_17530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HET6418101.1"
                     /translation="MKIKKLHIVGFKSFVDQTTVHFDHDITCVVGPNGCGKSNIVDAI
                     KWCMGEQSAARLRGKHMEDVIFSGSENRGPHGFAEVTLTFDNTDGLAPPEYSDYAEIQ
                     VTRRLDRQGNSDYQINRTPVRLLDVTNLFLGTGVGKRAYSIIEQGRIGFIVTSKPEDR
                     RSMIEEAAGISKFKLSKRAAERKMDQTRQNLLRVTDIIGELERSLASLKRQAQKAERY
                     KRYKKEMRDLDLWVASHRFLELRGQMGSVSASLSKATEDVQASRHALVAKEASVEAER
                     VLLQQMGFDVERGQARAYELDNLVRHLEGLIRQQEDRQAALHERKEIASRELRQLEEQ
                     RDALHAEASTVRLSLSELEALETQAGELLREVEAELQARKNSAEEADLALQNARSQLA
                     EGRARVAGAEAVLNGYEARRAEIRTRLERLRDELQTLSTREVEFSQQRGEHQARMVGL
                     RSGKSETSERQSEIETTLSTNKEALQQADSEVDRLREILAVTSSRLRSLNEIHERFEG
                     VGAGAQAVLTGYANATGHEQRSGLVGLVADRIECAPEHTRALGAALGGALEYLLVDDT
                     GAALRAADYLAREEKGRATFLPRSPSGAPRVVPQFPTGMGIVGPLLTLMRYSHADEAW
                     VRYLLFDVLVVDTLDTAARLHGQGFHGKLVTLDGQALLRDGSVVGGAKDESAAHLLRL
                     KREIRELGEAAAAEGAELERATARQGQLRASIAERQAAIDSVRVEAHDAELEILGAER
                     DLRHVDLELQRIGERRQEIAKAESEGRGSLSQVDVDEGKTRRELTDAQHLVSEAQEGA
                     RVAESVLVERRRVVDEQSAKATEVKVRATEARERADRERSMIGQLDRSIAELDAREDR
                     LREDVDVADRDLGQAAGRVLLHRGRLSETAHDASVAREELSRQRARFDEAQTLLGRSE
                     AALKELRSTIDKRSTELNALTLRERELTMSLEHLEEQVQDKHRIELAYVIGDHHARPV
                     PDAAITERAEELQGLIDRMGEINLTAIEEYEENAERYEYLTSQRRDLEEALTKLERAI
                     RQMNRESRAMFREAFTAINERFKRIFPMLFRGGKAELRLTDPHDMLETGVEIIAQPPG
                     KRLGSLELMSGGEKALTAVAMIFAIFQYKPSPFCLLDEVDAPLDEANIGRFAETIQQM
                     TQRSQFIIITHSKRTMEFADVLYGVTMEQPGVSKLVSVELRGDKRPVAATVEAAE"
     gene            complement(28551..29309)
                     /locus_tag="VFG22_17535"
     CDS             complement(28551..29309)
                     /locus_tag="VFG22_17535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0158 family protein"
                     /protein_id="HET6418102.1"
                     /translation="MNENDVGQDIEDIEDKEALDVPVAWEALEDAFENNAHEVHSYLH
                     FDTGEVIRIVDGVADPSMHTRIMNDKRYLRIDPVSSREQYRWMERFIATVEDEELRRR
                     LNIAIDGKGAFRRFKDALMSHPVDRERWFAFRSERLRSCMESWLTAHGIHSVERPTWH
                     VPTADEVHPEPDAPAVAPEGTPDLGSANELPLPAETGRTRLHELVDGLAVRDLEMAGA
                     FLEFLRARRHAPRPQVKAAPEPAVAAEAKSAETT"
     gene            complement(29306..30805)
                     /gene="glpK"
                     /locus_tag="VFG22_17540"
     CDS             complement(29306..30805)
                     /gene="glpK"
                     /locus_tag="VFG22_17540"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HET6418103.1"
                     /translation="MSGFLLSIDQGTTGSTALLLSPRGEILARANCEFPQHFPRPGWV
                     EHDLDEIWKSVGDAVGQAIARAGVSARDCLGIGITNQRETTVLWDRKSGKAVHKAIVW
                     QDRRTADRCRELKNAGKEEMFRQRTGLVLDPYLAGTKVEWLLDNVDGVRTRAEAGELA
                     FGTIDSWLVYRLSGGKVHVTDASNASRTLLYDIVKGRWDEELAAELHVPMALLPEVRS
                     CSEVYAVTSGVPWLPEGIPIAGMAGDQQAALFGQTCFEVGDAKCTYGTGAFLLMNTGS
                     EPVHSDNGLLTTIAWRLGDQTVYALEGSAFIAGAAVQWLRDGLGLIASSDEIEAKARE
                     VDSTGDVVFVPALAGLGAPYWDPRARGLIYGLTRDTTAAHLARATLEGIAFQIVDLVD
                     AMQADAKRPIRRLRVDGGAARNDLLMQFEADMLGVTVDRPENVETTALGAAYLAGMAV
                     GVFDGLGSVIDAYRIERSFEPQVTQPERDGHLRRWRDAVRRARSEMGIK"
     gene            complement(30855..31946)
                     /locus_tag="VFG22_17545"
     CDS             complement(30855..31946)
                     /locus_tag="VFG22_17545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418104.1"
                     /translation="MTGGSQRVYDLAAARPDGWFEAVLEQSDDFDKACQIIGRSTLAL
                     ALIAGARVLSITANPQSQDLSTVEFSIGQDPTVRQLPLSEFRQTIGQYLLVPLQGEGL
                     PDDPGVEALQMHIGGRYMLEAVLFQVQPLELRHELGLSEVTIEFNGARHVLTLDDLRD
                     ILDERVRSELGLHRREEVSIDLNVVDRAEEANERGNWSATVALLNPWLGPISMLLRTG
                     EAEGLSKEVRARLSTSLDLLGTAYAELGELDAANEVLRLGVQWAGESSEAGDLFLALG
                     RASVAAEKHGEAIGLLRRAIRLGASEHLSMPLLARSLSAREQWLAAMVCAERARELGA
                     ADSSIDSMTTELEEHLGEAWPRFRAWLSD"
     gene            complement(31948..32913)
                     /locus_tag="VFG22_17550"
     CDS             complement(31948..32913)
                     /locus_tag="VFG22_17550"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET6418105.1"
                     /translation="MLKGAHVLITGGAGFIGTALTARLAKDNHIRILDILRRNALSKA
                     GLDRHPNVELVVGDVRDMTAVRRAVTGCEYVIHMASIAGVDTVLKNPVMTMEISLEGT
                     MNVLRAANDCTGVKRVIDFSTSEVFGSYAFRVREADVTSLGAVGEARWTYAVSKLATE
                     HLAHNYWKQFGLPTVSIRPFNIYGPGQVGEGAVHAFVVRALQGEKILIHNEGDQIRSW
                     CYIDDIVNAILLAMEREEAIGESFNIGNPRSTITIYQLAQLIVRLTESKSPIEFVDWD
                     FPDVELRIPDVKKAEQLLGFRAHVELEQGLLDTIEWYRRKLSGEA"
     gene            33010..33690
                     /locus_tag="VFG22_17555"
     CDS             33010..33690
                     /locus_tag="VFG22_17555"
                     /inference="COORDINATES: protein motif:HMM:NF015945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HET6418106.1"
                     /translation="MGIFSRLNRVIKSNLNALIDQAEDPDKMIGQTVADMKAALKRAR
                     KDLIEAMGTVKRLESKERELDKEAAEWEQKAILALERDDDDLAREALRRKARTLSEAE
                     KVRARAAEQSTAAEAMRAQLERIEEKLDDLKSRQKTLAAQVRQARNQNPDPGQPTGER
                     LGGGAFSDLDRMADQIDQLDAEVEAHQLLDDPRRAELDARLRALETDEGDDAVDDELA
                     ALKAKLNG"
     gene            33703..36024
                     /locus_tag="VFG22_17560"
     CDS             33703..36024
                     /locus_tag="VFG22_17560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418107.1"
                     /translation="MERFVLWHRILPPAFEDEENTAAAGQWARYVTNQVQNGGGEVIS
                     SLAGTVVASFELHGLKTAINLALRLLTEAESQPVPAGGLPIAFGIATGDVQRVDDDTG
                     RLTNSGSAIDRAQLLANQARAGEVVLDVESREAASRTYLFGRSVSTSSFALHGEAIDR
                     SRPLLDECRRSVGLVHAAPIPASIRRALEPLEKAAAGSETIAFYLEGPLGIGARNGVF
                     ALRDELRPAVFLEIGAVPGGLEPLGGLRLALLKAWRSLEPTRQALKKVAPGVADAVKS
                     IACATPPQREAAVHSLKVMFRALRQEHALPWVYIDRISAVDPATLDVLGAALSDETVV
                     AVLLVRTTESSVPAGLRGLKRSTRIELPALEPSEALIVAAGVLGTDPDPEIAKQLVIT
                     GGTTVLGITEAARTMIATGDIVHEGGRFVWREADVERRQYLGPRALLEERFATLDTSS
                     MRFLEVACAALPASSAQVVDTAVALDGIPDESRHRALEALVNDGLLTPQAKPQSELLR
                     RAIVQRMPPARRTEVYRFVAQALHTAEPLLGPSMAATVGAYLCEGGDLVRGAHALLEA
                     GSLAADNGYTSAAVRLAAAAVQYQPDTETREGASEISRAVRLPSRPPEDEEEDRPTIP
                     VPEEELRNPTEEIIRVLRDRDHEKFDALIASAIASGGDLTGAHCLRVISYVIRDDLPA
                     AREALAAAKKSPNPTPGAPIRIAIASACIQLSRGRRRDAMIDALEALSTARSHQDGPG
                     EAAALKMVSATCLAAGLERDATRLAHVAEAVAS"
     gene            complement(36017..36880)
                     /gene="pheA"
                     /locus_tag="VFG22_17565"
     CDS             complement(36017..36880)
                     /gene="pheA"
                     /locus_tag="VFG22_17565"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HET6418108.1"
                     /translation="MNQTKETLLVGYQGTEGAYSQLAALRHFSGAAGSVRCVGYHSFR
                     EMLESLKAREIDYAVLPIENSIAGSINESYDLLARMGLSLVGEEFQPIAHCLIGAADV
                     PVSEIRRVYSHPVALAQCREFLETLEDCHVEAFVDTAMSVEKVKHDADPTQAAIASEQ
                     AAALHGLPILRRDIADHPENYTRMVIVAPEPAHYEHGVPCKTSLIFSTIHEQGALARC
                     ISIFAEQGLNLTKIESRPKPDTPFEYIFYVDFEGNVEREKTQAALHDLETVTTFLTVL
                     GSYPIRRAGVS"
     gene            complement(36892..37377)
                     /locus_tag="VFG22_17570"
     CDS             complement(36892..37377)
                     /locus_tag="VFG22_17570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HET6418109.1"
                     /translation="MPSFDVVSKIDQHEVDNARNQASKELAQRYDFKGTESTIEQTEE
                     GIVIRSNSEGRLDAARDVLETKMVRRQVSLKSLDPQKPQQAGGQMWRQLIKLKEGVSK
                     EKAKEIVKAIKDSKLKVQASIQGDSVRVSGKNRDDLQDTIAFLKEQDFDLPLQFVNFR
                     D"
     gene            complement(37402..38907)
                     /locus_tag="VFG22_17575"
     CDS             complement(37402..38907)
                     /locus_tag="VFG22_17575"
                     /inference="COORDINATES: protein motif:HMM:NF021382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acyl-CoA regulator family
                     protein"
                     /protein_id="HET6418110.1"
                     /translation="MAETNAAMGQRLRELRLRRGLQQSEAARRLKISPAYLSLIEKGK
                     RVVQLPLLFQALELYDVQVEDFMSSLGERRVDDGLAKLLDEPLLRSLNLSEEDLASIS
                     AEPRMITTITALFNLYKNTRSQLDHVMASVAQRERQAGDGSLRFDYSPYDEVTDFLEQ
                     HDNWFEQLEERAQQFRRDCAFGRRVTSDDLERGLLEQVGVRVHRIPERTDSSVIRTWN
                     AEERVLTVSSDLFEQRLKFQLAHTVALGLFDEEKLHEPILEGYSGQHGETRKLLKIHL
                     ANYFAGALLLPYPEFYREVTSSRYDVELLAQLFESSYETVAHRMCNLSDPRRRGAPMH
                     FLRVDVAGNISKRYSGDGIRFPHQDGSCPKMAVHLAFLTPSVITKQYSAFPDGSTYFC
                     FAKIVSEPQQGSLVKGTTYSIGLGTHADDAKHFAYADDLPFADPKKMAVPVGTTCRFC
                     ERTDCNMRSAPSYKFAFRVDEYTKKDNFFSPLVKGDSDGDSQKPGRRKLAP"
     gene            39143..>39585
                     /locus_tag="VFG22_17580"
     CDS             39143..>39585
                     /locus_tag="VFG22_17580"
                     /inference="COORDINATES: protein motif:HMM:NF013781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HET6418111.1"
                     /translation="MAEYGKRERDRPWIMRTYSGHSTAAASNALYRTNLAKDQTGLSV
                     AFDLPTQTGYDADDPLARGEVGKVGVPVSHVGDMRALFDGLPLDQMNTSMTINATAVW
                     LLSLYIAVAEDQGVDHHVLRGTTQNDIIKEYLSRGTHIFPPAASMR"
BASE COUNT         7409 a        12575 c        12196 g         7405 t
ORIGIN      
        1 ttgctttcgg gtcgtcgcga gcatcgcgtc acggggacgg gtatgcgttc ggcgagctct
       61 gcgccgaacg catcgtcgac gtggatgacg gcgtgcttcg gcgcgagctc ggtgaagaag
      121 cgtgccttgg cctcgccgta ccgctccatg ctcccgtgga agtccaggtg gtcacgcgag
      181 agattggtaa aggccgcaac cgcgaaatcg agcgcgtcca cccggcgcat ggcgagcccg
      241 tgactcgaaa cttcaaggac caggtgcgtc gcaccttgat cgagcgcaat acgcgcgaaa
      301 cgggagatat catcgccttc cggcgttgtg tgaagcgagg gcgtcgtgtc gcccaaaccg
      361 atgagacccg tcgtaccgac gagcgctggc ttgccggatg ctccctcgat cgcttgcttg
      421 agaagatacg ctgtcgtcgt cttaccgttg gtcccggtga ttccgacggt cggcagcgcg
      481 ctggtgggct cgccgtagac caactccgcc gcctgcccga ggcccagtcg ggcatcggcc
      541 acgatcatca gtggaacgtc cgaggcgagc tcccgctctg ccatcagggc gacggctccg
      601 ctgcgcaagg catcgcgcac gaagctcgat ccatcgtggg tggcacccgg cacggcgacg
      661 aaaaggtcgc ctggctcgac gcagcgcgag tcgtgctgca cgccaatcac gcgctgactg
      721 accgatcccc ggattcgtcc gacgacaccc ctgctctgta gttgctcgag cgtcatgccc
      781 gtcttccatt cagggcgcat gcggcctccg gggtgccgcg gcggcgagct tattcgtgct
      841 tccaccggac gtcgcagcct cggtatcgaa gcgctgacgg tcgagacgca gccgaacgac
      901 cgccccgtgg gcaaccgctt tgcctggcgc aggatcctgc gcgaccacga ggcccgatcc
      961 ttggacctcg ggcaccaaag actgggcatg caacgccatg acggcctgcc gcaaaggcag
     1021 cgagcgcaca tcggggacgg cactctcccc tttttcgacg ggctcgtccg gcgccgcagg
     1081 ttcgacctcc gagatcgcga tccgttcttg gtgctttttc gcaagaaccg cctgtcgccc
     1141 ttcgggtgcg attcccaagt gtcgaagcgt gacttcggcg attcgccgga acaccggacc
     1201 tgcaaccgtt cccccgtagt gcgcaatggc gggctcgtcg atgaccactg aaatcacgat
     1261 cgcaggccgg tccgcgggcg cgaatccgat gaacgacgca agccattggt ccttggcgta
     1321 cccgccgtga acgtagtctg ccttctgcgc cgtccctgtc ttgcccgcta ctaggtagcc
     1381 gtccaccgcc gcttcgacgg ccgtgccgcc aggctcggtc accgcggtga gcatttgccc
     1441 aaccagtttc gccactcgcc tcggaacgac ctgccgacga acgcgcggct tgttttcttc
     1501 gatcgtcgca ccgtggccgt cggtcatgcg gcggacgaga agtggttgca tgagttgccc
     1561 gccgttggcg atcgcactca tcgcggtcgc cagctgaacg ttcgtcacac tcatcccctg
     1621 gccgaacgaa accgtggccg tgtcgatctc gtaccaacgc ctgtagtgac gaagaatgcc
     1681 tgcggtttca cccggcacac caagaccggt cggctctcca aacccgaaac gcctcaagcc
     1741 gcgatacaac tccttctttc cgagatcgag ggcgatcttg gccgtgccga tgttgctgga
     1801 gtacgcgagg acctgcgttg gagtgagcca ttcataagga tgggcatcgt ggagcaggcg
     1861 gccacccaag cgcgtgaccc cgttctcaca gttgatcgat tggtttggtt tgacggtacc
     1921 cgcggcgagc gcagcggcca tcgtgaacgg cttcacggtg gagccaggct cgaagcggtc
     1981 cacgacggct cggttgcgcc gatgcgccgt cggatgcttc gagggctcgt tcgggttgaa
     2041 aggaggatag ttcgcgagcg ccagaatctc tccggtcgat gggtccatga cgacgacgct
     2101 tccgccgcgc gcttcgaagg ttcgcactcc caacgccagc tcgcgctccg cgatgtgctg
     2161 gatcgccttg tcgatggtga ggacgacgtt ctgcccttgc atcgagcggt cgtcctgcat
     2221 ctcgtcggcg aagacgacct gaccccggcg atcacgtatc gcctccacgc gccgatcgga
     2281 accccgaagt cgatcctcgt atgcgagctc gacgccttcg attccacgcc cgtcgatgtc
     2341 cgcgaatccg agcagatgag ctgcaaggtg gcggttcggg tagtagcgcc gcgcttcggt
     2401 ggtcagctcg acccctggaa tgtccaactc gcgaatgcgc gtagcctcat gaggtgtgac
     2461 ctggcgttcg atccagacga agtagcgatc ggaagccaga cgtcccgcga gcttctgggc
     2521 gtcgacgtcg agaatctgtg cgattcgcct ggccacggtg cgtgggtcct gctccatcgc
     2581 cttgagccgc ttgggatttg cgtagacact gtcgacgtcg gcgctgaccg cgagctctgc
     2641 gccgtgtcgg tcgtagatga ccccgcgcct tggcgagacc cgaagctcgc gaacgtgctg
     2701 gctctcggcc atgccacgca atcgatcgcc actcgcgatc tgcacgtgga aagcacgaac
     2761 catcacgatc atcgcgacgc ccatcacgcc tataccgagg agcgcaatgc gtgcccgaaa
     2821 ccatcgctgg cgacggttct tcatcgcacc ctcccggcag cggagcgcgt cttctgttcc
     2881 ttcatcgtta cgatgcgtgt cggtttaggg acttccattc cgagtgttcc gcgcgcgatg
     2941 gtctcgattc gaccgctctg ccgaagggtc gcggcttcta tcgaaagcag acggcggtct
     3001 tgaaggagct cactttgacg gccgcgctct tcgctcactt cgtatccgag tcgaactgtt
     3061 tcgaagcgaa tcgaaagatg cacgacaaac gccgcagcag tcgcgaacac cgcgacgcac
     3121 cagagcggca agaaacgccc cttcacgccg caacctccaa tcggcgggcc gcgcgaagtt
     3181 tcgcgctgcg tgatctcgga ttggcctccc gctccgactc cgaagcgatg atcggacgct
     3241 tcgtcagagg caccagatgc tcgctttcgc gaaagccgtg cttcacgatt cgatcctcga
     3301 gggagtgaaa actgatcacg gcgattacgc cgccaggtcg cagcacgctc ggaagctgct
     3361 cgagcaagga ttcgagctcc cccagctcgt cgttgaccgc gatgcgaagt gcctggaagg
     3421 tcttggtcga tggatcgata cgaccgcgac gagggccggt cgcgcggtgg acggcacgac
     3481 gcaagtcgcc ggtcgtttcg agctcgcctt cttcgtaggc atgtcgaagc gcccgcgcga
     3541 tcttccgtga tcggtgttcc tcgccgtacc ggtagatcaa gttcgcgagc tcttcggcgt
     3601 cgctgtaggc gatcaaatct agcgcgctat taccttcggt cggatccatt cgcatgtcga
     3661 tgggtccgtc atttgcgagt gaaaatcctc gctcggcctg atcgagctgc gcagagctga
     3721 cacccaagtc ggcgacgatg ccgtcgacct gctcgatatc gagcgatgtc aggacgtcgc
     3781 ggaggtcccg aattcgggct ttgcgtagtg tcacccgctg tccaaattca gcgagctccg
     3841 cgcgcgcagc atcaagcgcg ctggggtcgc ggtcggttcc gatcagtcta ccatcgggtg
     3901 cgctgcgctc gagaatcgct cgcgcgtgcc ctcctccacc aagcgtgacg tccgcatagg
     3961 tgccccccgg cgcgggtgcc agttgttcga gaagttctgc aaggagaacc ggcgtatgct
     4021 cgaagcctgt cacagcccga gctccgcgag ccgcttggcg atcgccaagc gccgatcttc
     4081 gtcagcgaga acgtctttgc gaagatcttc gaagctcgcc ttggcccaaa gctcgatgta
     4141 gctgcccatc cccgcccaga gcgcatctcg cttcaaatct gcgtgcttgc gaagagccga
     4201 agggatcagc aatcgcccga ctctgtcgac gtcgcactcg acagcgccgg aaacatagat
     4261 gcgcttgagc gtaacgacgt cggtgtcgaa ctgcggaagc tcggacaggc gcttctcgaa
     4321 cgccagccac tccgccatcg gataagcaac gagacaggtg tccaatcccg tcgtgaccac
     4381 gagctttgcg tcaccttgcg aagtcatcac ctcgcgaaag cgggacggga cggaagtgcg
     4441 ccctttcgca tcgatgctgt gctcgtagcg tcctcggaac aacctaagcc tctcgtccta
     4501 gtcagtagcc aattggcact tgatcccact agttcccact aaggctggaa actacggcgc
     4561 aagctcatcc catgtcaact tctttctgct ctccaagtga tagaattcaa tggacttttt
     4621 gggcacacac gggaagctct ggacggcggc cccggggtgg gagacacgcg cccccgtttt
     4681 gagaacgtcc ggcagtctaa acgcgcttcc ccagaacgct gcggagcgca atattggcgt
     4741 taatcgggat gtaatcggcc tatattgtca tcgtgctgcc atgctattct ctgctctggt
     4801 ggatccgatc gacgaggcct gggcgcaggt tgaggctgga tggggggacg aagaggctca
     4861 tcggcgcttc gtcggcgtct gtgttgcact cgaccggctg cccgaggccg gtaaacgcta
     4921 ccggatggtt cgggagaccg atccgatgcg cagcgaggcc gcatccaagc aaatcgacag
     4981 gctgatcggg ctggctacgc agcaactcca agacacccga gtcgcaccga gcggcgtcgc
     5041 gcacaaaagg acgctgacat gggctgcgtt ctttatcatg ttggtgttga tgggggccgg
     5101 ggtcttgttc ttgctacgcg cttagtcgtc gcgttcggaa cgaacctccg attcggaatg
     5161 caagttgatt tcgctgcaaa ggaactgacc gtaaagctgg tctattacgg accggcgttg
     5221 agcggaaaga cgacgaatct gcgagccatc cactggctgg gaacttcgaa ggcctgtggc
     5281 gagttgatga cgctcgagac tcagaacgac cggactctgt ttttcgacat gcttccgatc
     5341 acggtcagct cggagagcgg catcaaggtc cgtctcaagc tgttcaccgt gcctgggcag
     5401 gtaatgcaca acgcgacgcg cagactggtt ctgcaggcca ccgatggcgt cgccttcatc
     5461 gcagactcac aacgaagcga ggccgccgcc aatcgtgaat cgtttctcaa tctcaaacga
     5521 aatctcgcag agaacggaat cgatcccgac cgtgtaccga tcatcattca gttcaacaag
     5581 cgagacatgg acgacatccg cacggacgag gagctcgaag cgatcgcgag acagagccgc
     5641 gaaccgatct acacagccgt tgcgacgcgt ggctacgggg tcatgcagac gctccgcgga
     5701 ctgattcgcg agacatggag caagctggag gaggagcacg ggcttcacga gaagttcgga
     5761 gtgagcccgg ttcgattcat gcaagaggtc gaatcgaagc tgaaacacga tcccaggcag
     5821 caggagcgat gatgcaggac gataccaatg ccgcggatat cttgatgggg gatgtagtcc
     5881 gcctggagga cgtgctcagt cgcgactcgg tgacctcggt gtcgaaatct ttcttcaccc
     5941 tatttggact ccccgttcgc gtgatttccc acgaagggga tctgctggcg gacgtccatc
     6001 gcgaccgccc tctctgcaac tacctcaaca cctcgcccgg gggtgagcgc gcgtgtgcgc
     6061 agacggtcgg tgccgttcga gatctcgagc ccgacggcga aaccatcgtg cacccgtgct
     6121 tcactggtgc tgtctaccgg attgttcccc ttcactatca aggacgtccc attggccgct
     6181 tcatcatcgg cccttatgtt cccgcggaaa ccaaacgcgc ccccgcctcc ctcacagccg
     6241 tcgatccgtc catcgacgcg ggtgccgcac gtggacactt cgaccgtatg ccacgcgtgc
     6301 gcgaggacat cgccgagcag ctctcggccc acctcaaggg cgtcctcgag ctactcgtct
     6361 tctcgggcca ccgcgcacaa ctcgcttcga ccatgcaggt cgcaagcgtc cgtgagaact
     6421 accgggagct cgcggaaaag aacgaggcac tcaagacttc ctacgacgaa ttgaaacagc
     6481 tcgatcagct caagtccacg ttcctggcca cggtgagcca cgagcttcgg accccactga
     6541 cttcgatcat cggctacacc gaaatgctcg aaggtggagc agcgggcgca ctgaatgaag
     6601 gccaatccga cttcctcgag acgattcgag ggaaagccga ccaactactt ggcctcatca
     6661 gcagcctcct cgacctcggg cgactcgaag cgaagaactt ggagctcctc aacgaggcgg
     6721 tcgaccctcg caccctactc tcggacatcg gctcgaccat cgtaccgaac gcaaaccggc
     6781 gcaacgtcgc gctggagatt cgagtcgcga gcggcgtacc gaaaatttgg ggcgacccgg
     6841 tgcgactgcg ccagatccta ctcaacctcg ccgacaatgc cgtgaagttc accgacgcag
     6901 gaggcgaagt cgagctgagc gcagaagcgg gcgagctcgg tggagggacc tcttcgggcc
     6961 tcggcgcagc cctcttcgcc gcaagccgac cagcggtgat tctgacggtt cgagacacgg
     7021 ggatgggcat cccagaggat aagctcccgc gaatcttcga cgcgttctat caaatcgatg
     7081 cggggaccac acggacccat ggtggtgccg gactcgggtt gtcgatcgtg aaacagctcg
     7141 tgaacggcca cgacgggaag atcgaggtga ccagctcacc cggcgagggc acaatcttca
     7201 ccgtgacgct tcctgccgcc cacgacgatg attgatgagt tcgccaagat cgagtggctg
     7261 aaggcgcgct tcgagctcga cggcgactcg aacgggatcc tcgtcggcat cggtgatgat
     7321 gcggctgtga tcgactttgg tgatcgtcca agcatcgtga cggtcgatgc tcatgtcgag
     7381 aacgtgcact ttcggcgcga cttgatctcg tgtcgcgagc tgggctaccg agcgctcgtt
     7441 gcagcagtga gcgacgtctg ggcgatggcg gcggtgccga gggctgcact gatcgcgctc
     7501 acactccccc cgagcttttc cgacggcgat ttccgtgagc tcatcgaagg gatcgccgag
     7561 gcagcgaccg gcacgggcgc caaggtcatt ggcggcaatc tgagcgcgag cgagtccctc
     7621 accgtaacca cgacggcctt gggaacgccc ctgggaaagc cgctcatgcg agcgggtgct
     7681 cgaccaggcg acgcgatcta cgtaaccgga acgctgggtg ccgcggcact tgggctcgca
     7741 gtcttggagt ctcgttcttt cgggctcgaa catggacgcc gattcgtcga gcgatggaaa
     7801 cgaccaccga tcaacggcta cgcgttagac gtattggccg cggtcgcgac ggcagcggtc
     7861 gacgtctcgg atggcgcgct gcaagacctg ggacacctct gcacggcctc cggagtcggc
     7921 gcggttcttc gcgcgagctc agttccgacc gcgacaggct atgaaaccag ctgcgcggca
     7981 ctcgacctcg acccaaccaa gctctccctc actggcggtg aggactacga gctgatattc
     8041 acggcacccg agtgctccga ggccgaaaag atcgggagca agatcggaac ggtcatcgaa
     8101 ggcaatggaa cgcgtgtgct cgatgaagca ggcgagcaga tcgaccttgg caatgctggg
     8161 ttccgtcact ttgcatagag ccaagcggag cgacgctcgt catcaaatag cgccgcacca
     8221 cgcgcttcgt tcgcaagcac cgtgtcgagg gatgctcccc cgtcgacagc tgtgcggaac
     8281 tcggggccac cggtgagaag atcgatcgcg gggcgatccg agacgaactc gtagacatcg
     8341 gttcgccagc gttctactgg ctctagctgc ggaagcacgg ccgcaagcat tcggagatag
     8401 gcttcgtatg gccgaaagac ccatggatca gtgacgtgca cctgcacccc agcacagctt
     8461 aggcccgcgt gtttttggaa agtcggcgtc agctcgaccg gtcgcagcgt aacgccgtgg
     8521 aggtcgagct cgaggagcgg ccgaacgtcc aaacctggcg cgccccagag ctcgaacggc
     8581 ctcgtcgttc cccgcccctc cgacagcctg gtgccctcga ccaggcaccc cccgggatat
     8641 accagcgccg tgtcgggtgt gggcatgttg ggagagggca tgacccaggg cagaccggtg
     8701 tccttccaat tcatcgagcg catccagccg tccagcggga cgatgtttag gacgctgcga
     8761 tcgatcttct cgagatcgca gacccaatgc atgagctccg cgatggtgag cccgtgtctg
     8821 acggcgaccg gatagagtcc cacgaacgac agatagccat cgcgttgtgg ggcaccttcg
     8881 acgcgtgcgc cccccagcgg gttcggccgg tcgaggagaa cgacctcggt tccgaccgcc
     8941 gcggccgctt tcatcgtcag agccgcagtc catacgtagg tgtagtagcg cgacccaaca
     9001 tcttgcaggt cgatgacgat cgcatccagg ccgcgaagcc attcgggctt cggactcagg
     9061 tcgccggaat cgtctccgta aagcgagtag atcgggacct cgtcctggtc gctcgtcccc
     9121 acgccaacca tgtcttgcgc tgcgcctgcg aagccgtgtt cgggcccaaa cagcgcagca
     9181 acattggctc ccgccgcgcg aagaacagaa gcggcgggcc gaagccggcg atccacgcta
     9241 gccgcatggg cgagcaagcc gacgttccgt cccgctacga gcctaaccgc atccaagtca
     9301 ccatcggcga tgcggtcgag acctgtgcgc accgccgtca atccgcctcg cctaccgcag
     9361 gggcgtcact cgtttcacca gcgaagagat agaggagcgc cattcgcacg gctacgccgg
     9421 cctcgacctg atcgagcacg acgcttcgtt cgctatcggc gaccacgctt tcgatttcca
     9481 cccctcggtt catcgggcca gggtgcatga tgatcgcgtc gggcttggcg cgtgcgatgg
     9541 cccgaggacc gatcccatac gtccgcgcgt actctcgcag actcggcaga agtgcgccct
     9601 ccaagcgctc ttgttggatg cgaaggacca tgacgacatc ggccccttcg agtgcctggg
     9661 ccggctggtc gaaaactcgc acgccgagcg agtccaccgc agcagggagc aaggtacgcg
     9721 gaccgacgac ccgcacttcg caaccgaaca gcttcaagag gtgcgcattg gaacgagcaa
     9781 ctcgtgaatg ggcgatgtca cctactatcg tcacgaccaa gttgtcgagg gttccttttt
     9841 cgcggcgtat ggtaaacgcg tcgagcaatg cctgggttgg gtgcgcgtgc atcccatccc
     9901 ccgcattgat gacgctgcaa ccgatcttgg aggcgacata gcttgcagcc ccggacgcac
     9961 tgtgccggat gacgatcacg tccggatgca tggcttcgag tgtccgacac gtatcttgca
    10021 gcgtctcgcc cttcttcacg ctcgaggccg acaccgtcat gttgatgacg tccgcgctta
    10081 gccgcttgcc cgcgagctcg aaggaagttc gggtccgtgt agaggcttcg taaaagagat
    10141 tgatgactgt cttgccgcgg agcgtcggga ccttgcgcac agggcggcga gaaacctgta
    10201 ggaaggcttc tgcagtatcg aggatgcgct cgacatcgac gcgcgtgagg ccctcgatgt
    10261 ccagaaggct acggtgcgca aagggtttca ttcgtcgccc tccgagagca cgacgcgatc
    10321 gggacccgct gcatcgagcc agacgtccag acgcccgccc gggtccatgg agatcttgcg
    10381 tccgacgaag tccggcgcaa tcggcagctc acgcccccct cgatcgcaga gcgcagcaag
    10441 ccaaacgcgt gccgggcgtc cgtggtcctg gagacattcg atggcggcgc gcacggtgcg
    10501 ccctgtgtga agcacgtcgt cgacgagaac cacatggcgg ccattcacat cgaagggaat
    10561 gtcgctcggc cctatcttcg ggttgggaag cgcggtggcg gcatcgtcac ggtatagaga
    10621 gatgtcgact gtcccaacgg gtggaggcct accctcgacc gctgcgattt cgtcggcgat
    10681 gcgccgcgac agcgcaacgc caccgcgacg aatgccgacc agggtcagat cttcgatgcc
    10741 agaggtcgcc tcgagaatcg cgacggcgat acgtcgaatg cttcgttgga tagcgtccgc
    10801 atcgagcacg accttctgat ttcgagccac gttcggcgga gtgtgtgtga aaaccagcga
    10861 ggcttcaagc ctaggcgacc gagaggcccc catcgatcgt gaccagactg cctgtctgcc
    10921 aggtctggtc gagtaccgcg acgatcacgg ccgcaacctc cctgggctca ccgagccggc
    10981 ccatggggtg caaggcagcg agtgccttca gctgggccgc aacccgcgtg tcgagctcgt
    11041 ccggtgcgag cgattccccc gggcgcaatc gtgggttgcg caacatgtcg gtttcgaccc
    11101 caccgggaag caccgcgttg acccgcacgc cgaatcgcgc aagctcgagc gcaagggtct
    11161 tggtcatcgc gttgagcgct gctttgctcg ccgcatagac gagtgtgttg ggagctacgc
    11221 gctcggacaa ggtgctcgac acgttcacga tggacccgcc ccgcgtgccg aagtgctccg
    11281 caagcgcctg tgaaaggaga agcggcgcaa cgaagttcac gtggagttgc gcctcgatcg
    11341 atgcgagact gatggcgccg acgggctcgt accgggcgat gccggcgcag ttcaccaggc
    11401 catcgatggg cccgaaggca tcgatcgcgc gtttcgccgc gtcttgaacc cgcgccacgt
    11461 cttcgaggtc ggcagacacg gcacgaatgc gtccctcgga ggatgcgacg agcaatcgca
    11521 aggcctccgg atcgcgagcg accgccacga cctttccgcc gcgcgccagg atcgcgtcga
    11581 ccgtggcccg gccgattccc cgcgtcgctc cggtgacgac caccgtgccc tcggaaatca
    11641 tcgcctcagc cccacccggc gatcgggccc cagggcgcct cggccggaac gtcggccggt
    11701 tgtccggtca cttcgatttc gtcttcaggg atgaacctcg gcgaatcgac gcagagaatg
    11761 ttctggtgac gttcactcgg attcatgtac gtatgcgccg cgcctcgcgg ccagcgatga
    11821 accgttccag gcgcgcaggc ctgcccctga cagagcaggc cgttgctcag caccatttcc
    11881 gactcgcgca tctcgtgatg cacgtgcaga ggaatgctct tcccgggcgc gacgttcaga
    11941 cggtagatgc ccgcgccttt ggtttcatag atcacatcga cggtcccgaa gggcttctgc
    12001 tcgacctcga cgctgcacca ggatgcgtct cgctcgattt cgagcgaagg aatcgcgaaa
    12061 cctcgcaggg cgccgggctt gctgaggcgc accagagcgg aatcgacctg tgcgcggcgc
    12121 tcatcgggcg caggtggcgc gaggatgtat cgcgccagca cgtgggcagc ggtttccagc
    12181 aaccgaaacc ggcagcttcg cagcaagaag acgacttgtg aggcggtcgc cgcatagtcg
    12241 atcgtcgaac gtaggctctc cttgaccgca gacgcctcgg tttgcagacg catttcgatg
    12301 tcgacacgga gtggctgaga ggcgttgcgc tcgtgtgggt aaacgccgac gacacagtcg
    12361 accatcagct cctcgatgcg caagacatcc atcgggcaag gctatcgcat ttcgggactt
    12421 ctgctagagt gccgctgcca atgagccagc ctgattcgat ccccacgaac gagatcgcgg
    12481 aagtcgctgc atttcggccc gttccgcgca ccggcgtgat ctacgtcacg gtcgaagccc
    12541 agaagcgggg tttcagcctc gacgcgccgg actggtgcaa cctcggacaa ggccaacccg
    12601 acacgactgc gcttcctggt gcaccggata gggtaacgaa catccctgtt gaacctggcg
    12661 acctcgacta cgccccagtg ccgggtattt gggagctgcg cgaggacgta gcgggcctct
    12721 acaacaagct ctttcgccga ggaatgaagt cccagtactc cgcggagaac gtcgccattg
    12781 ctggcggagg ccgcgttgcg gtcatgcgcg cctgcgccgc gatcgcgccg attcacctcg
    12841 gacacttcct tcccgattac accgcgtacg aggagctgct cgacgtgttc cggcggttct
    12901 tgccgattcc cattctcctc gacccgaagc aagggtacga gttcacgcgc gaacaactgg
    12961 aagaggagat tctcggtcgg gggttgagcg cgatcttggc cagcaaccct tgcaatccca
    13021 ccggcaaggt cgtccaaggg gacgcgctcg caggttgggt ggagacggcg aggaattcca
    13081 actgcgcgct gttgctcgac gagttctact cgcactacgt ctggaacgac gaaccgacga
    13141 tcgtgagtgc agcgcaatac gtggaagacg tggacgaaga tccgatcgtg gtgttcgatg
    13201 ggctcacgaa gaactggcgc tacccaggtt ttcgcgtcag ttggatcgtc ggcccgaaac
    13261 gggtgatcga atcggccgca agtgccggct cgttcctgga cggaggcggg gtcgccccca
    13321 tgcaacgcgc cgcgatcgat ttggtccagg aagccccgac gttggctgaa gcggccgcag
    13381 tccacaccga gttcaagaag aagcgggact tcctagtgaa aggcctgaag gacatcggcg
    13441 tgcgtttcga cctcgagcct cagggcactt tctacgcatg ggggaatctg agcggattgc
    13501 ctccttcgat tcgcaacagc gacgactttt tccgggctgc gctagatcgc aaagtgatct
    13561 gcgtcccggg gcacttcttc gacgtgaacc ctggcaaacg gcggaccgga cgcccatcgc
    13621 gcttcatcga gcacgtgcga ttctccttcg gcccgccgat gccggtgctc gaagaagcgc
    13681 tcgaccgcct ccgcgcgatg cttgccgagg ccacctagtc ccaggccaca tagctgaatt
    13741 tacgcgataa ttcgccgtca gctcgccgct tttttccgct ccgccgtctg tgtaggcgat
    13801 catgctacat atgacgacat ctgcagcggt cgcgctcagg ctagagcagc agccaaggga
    13861 ggcagcccct ttcgtgaagt gggcgggggg caagggcaga ttgctcagcc agctgcgacc
    13921 gctgctccct tcgggggtag agcacatgcg ccacgtcgag ccgtttgtcg gcggtggcgc
    13981 gctctttttt tcgcggcgcc ctgggcgcgc gcttctaacg gacatcaatc cagctctcgt
    14041 cgggacgtac gtcgcgattc gcgatgacct gaacggtgtg atcagcgcct tgcgtgggct
    14101 cgcaaagggc cattccaaag aaggctacta ccaggttcgc gaacgctaca accaggggcg
    14161 gcgcatctcc accgcgaaac gggcggcgat gttcatctac ctcaacaaga cctgcttcaa
    14221 tgggcttcat cgcgtcaacc gcaaagggga gttcaacgtg ccggtcggct cgtacaagaa
    14281 cccacgcatc ctgaacgagg aggggctcca ggccgcgagc caagcgttga aacatgcaaa
    14341 tcttcagtgc gcgcctttcg acgctctact cgaaaacgcg aagccggggg acttcgtcta
    14401 cttcgacccg ccctacgagc ccgtgtcccc aactgcgagc ttcacgtcgt atgcgcgcga
    14461 cggcttttcc caggaagatc aaactcgtct gcgtgacgtc tacaaggccc tcgaccgtcg
    14521 aggctgtaag ctcatgctct cgaacagcga tgtccccttc atccgagagc tctacgccga
    14581 gttcgaagtc gagacagtgg cagccccgcg cgcgatcaac tgcgacgcga gaggacgcgg
    14641 caaagtccgc gaagtcgtcg tccgaaatta cgtctgagac tggcactggc tctgggggag
    14701 tgatgcctcc agctgtacgg atcgcagggc ggctcgcgac gcacgtgctc gcgattggga
    14761 cccgctcggc cgtctcttca agtttcccta cgtctagcct cccgcccaat cgctgtgccg
    14821 cgcatcccga gccgccctcc tatggagacg ccagcgcatg aacactggca ctggtgctgc
    14881 acagagcagc gcaggtgttt tcgatagtcg tccggcagcc ccttgccaag ggcaccgatt
    14941 cgagggaacc tgggctcgtg cgtgcagttg ccttggtttc agtgcttggc ttctcgctcc
    15001 ttgcctgctc acaggaagcc gagtcagacc agctcacgcc aacgctgaaa cattcattcg
    15061 agcctctcat gatcgctgca ggcgacgaga tgactggcgt ttgtcagagc tggaccctcg
    15121 acaacgacga gccactgtac gtctccaaaa ttcggcagcg caacgacggc gcctggcatc
    15181 actccaattg gttcttcgtt ccggaaggca cgtacgccgg ccccgatggc acctggaact
    15241 gcgacgaccg cgacttccac gacgtcacgg cagctctcga gggaggcgcg ttcttcgcgc
    15301 agtctacaca aaccctgcag gaggttcagg cgttccccga cggtgccgtt ctggaaattc
    15361 cgccgaggca caagatcgtg ggcggggttc acctcgtcaa cgtatcagcc agccccctcg
    15421 aaaccgcgct cagtttcgac atcgagaccg tctccgaaga tagggtggaa gtgcggctca
    15481 ggccaatcgc gttcaccaac accgcgctcg acatcgagcc gcaggcggaa tccagattct
    15541 cgatgacctg tgacctcggc aaggagttcg aacggttcct gggcgcccct cccaactaca
    15601 acatctacta cgttctggcc cactaccacg agtggggaaa ctattttcga ctcagcttcg
    15661 tcgatgagac gggtggcgat cgaacgatct tcgagctcga gaactcagtg ggcgagcctc
    15721 ttggcatcac tctcgacccg ccgatcaatt cggagaacgc tcccaagctt cgggtcgaat
    15781 gtggctactt gaacaacacg gatgaacgtc tcaagtatgg tctcggaggc aaggaaatgt
    15841 gtgtattcct cgcctatacc gacgccgacc tgacgttcgg cgctacctcg ggcggcaaca
    15901 atgcactcgg gccgaacgaa gacggcatct acatgaacga aaccgattgt ggcatcgtgc
    15961 tcgccgttcc cgcgaactga catcggcgcc atgaacgtga atgtcacacg ccgcttcgag
    16021 cgtcggtcac cgtcgaatga gcggacgccc tgctgcgcgc tgctcgtccg cggaataagg
    16081 gcagtgtcgg caaccggagc cgcaacactt ccggcgggcc ctgagcgtgt ccgccgtcat
    16141 gacatatagc cctgtcctcg gatcgaggta gccttcttcg ccgcgcgcca gtgccgctcg
    16201 gtgtgcgtca actatgtcgg gcgtgagtgt ttcgtccagg gcgcgcccaa cctgctcgtc
    16261 gatcgggggc tcgtcgcgat ctttcatgtc gcattccgat cgagtcggtc agccatcgac
    16321 gtagattcgc ggcacacggc gtgagacgtt ggtcagaact tcatagggta tcgttcgggc
    16381 ggcggcagcg agatcgcgcg cgtcgagaac agctcctttt tgctcaccga gcagcaagac
    16441 ctcgtcgccg atcgcgacgc ttggaatcgc cgaaacgtcg acggtggtga gatccatcgc
    16501 gatgtttccg acgatcggac atcgaacgcc gtcgatcagc acctggccgc ggttggaagc
    16561 cgcgcgcaag aggccatctc cgtagccaac cgggatggtc gccaagagcg tcggtcgagt
    16621 cgctcgaaac gatcgaccgt agcccgccga ctcacccggc tggagggtgc gcaaacgaag
    16681 gatttcggtg cgccagcgca tcgcgtgtcg aagcggtgcg tcgaccgtgt cggcgcctgg
    16741 gtacccgtac agcgcgatcc ccggacgcac ccagtcgaat cgggattcgg gatgccgaaa
    16801 cacggcggca ctgttggcgg cgtgcaggac cgaaggctga tggccgaatc gacggacgag
    16861 gccttcagct cgagcaaacc cggcaagctg ctcggccacg tactcagggt ccgtatccgc
    16921 agtcgagaga tgggtcatga ggcccgcgat tcgaatcgaa ggaaagcggc gaagctctcg
    16981 aagaaacacg ctgagctcgg cgaccgggac gcccagacgt cccatgcccg tgtcgacctt
    17041 gagatgcacg tcgatgggcc gcccgtccga gaccgcatcg aaagcactcg cttcagagag
    17101 ctcgtagagc actggcgtga ggccgactgc gatgatgtcc cggtgcgcac ctccactgat
    17161 cccgttgagg accaagatcg ctcggtcgat tccagcctcc cggagctcga tcccttcctc
    17221 ggcgagcgcc acgccaaacc cgtcgacgcc atctctttgg agtcgacggg cgacgggcac
    17281 caccccgtgc ccgtatgcgt cggccttgac gaccgccagg atctttcgtc caccggccgc
    17341 gctacgaagc acgcgcaagt tgtgcccgat cgcatccagc atcacctcgg cgcgcgtggg
    17401 acgcagcgac ccgtcggcgc tctgcacgcg aggcctcagc ggagtcggga agtcggattg
    17461 cacggcttgg ggctaatggt tgaacccacc ggcgtcaagc attggcgtcg catcgtccga
    17521 aggctaggct cccaaccgaa tggcagggac tgagcgacgg atgcgagagg ccgcgaaacg
    17581 ggcccaacac cggtcttccg gccttctttg ggctggcatg atcgccgccg ttctgatcgc
    17641 agtcggcgtc gtggtggctc cgggcttgcg caacgcaggc gaagagacgg tgagcccggt
    17701 cgacctcggc gctgcggaag tctcgccggc tccggttctt ccgcaggtag aacagctcag
    17761 ggtccacgtg attcggaagt acccgcacgc gacggatgcg ttcacgcagg gtctcatttg
    17821 gcacggcggc gtcatgtacg agagcacggg tcagtatgga aagtccgagc tccgtagggt
    17881 tcgactgaaa gacggcaacg tgctcgcaag acgaaagctc gacccgggat tcttcggtga
    17941 aggcttggca ctggtggacc aacgcctgat tcaactgact tggcgctctg gcatcgcatt
    18001 cgtgtccgac gtcgccactc ttcaggagcg cgaaactctg cgctacgccg gtgaaggctg
    18061 gggcctctgc tacgacggaa cgtcgctggt gatgagcaac ggctcctcaa ctctcgattt
    18121 ccgtgatccc gggtcgatgg cgctaattcg aaaactcaac gtcacgaaag atggtctgcc
    18181 agtcgacgag ctcaacgagc tcgagtgtgt cggcagggag atctacgcca acgtgtggcg
    18241 gcacgacgaa atcgtgcgga tcgatcgggc gacggggcgc gtcacgggca gcatcgacgc
    18301 aagtggattg ctgacgcgca tcgaatcgag gcgcgcggac gtgctcaacg gaattgccta
    18361 caagccggac acgaagacgt tcttacttac cggcaagcta tggcctcagg tcttcgaagt
    18421 ggatttcgag ccgcgttgag caggaccgcg gacctcgacg tcgggttccc agctcgcaag
    18481 gcgatcgcgc atcgtcgtga tggcctccgg atgctcgtcg acgagaacga aaccacgccc
    18541 cagtctagcg gcagcttcgc ccgtggtgcc gctcccggcg aagaaatcga gaatggtatc
    18601 gcccggctcg ctatgaacct tgatgatccg ctcgagcacg cccagcggct tctgcgtggg
    18661 gtacccggtt ttctcactcc cattggtggg tacgatggtg tgccaccaga cgtcggtcgg
    18721 cgtcttgccg cgagcagcct tctcttttcc cacgagccca ggcgccatgt agggaatccg
    18781 gtccatctcg tcgaactgga agacgtaccg tgctgggtcg cgcacatacc atagaagcgt
    18841 gtcgtgcttc gcaggccaac gtcgctttgg acgacctccg aagtcgtacg cccaaatgat
    18901 ctcattgaag aagcaatctc gcccgagcaa cccgtcgagt gcgaccttca cgtagtgcac
    18961 ttcgcgagaa tccagatgaa cgaacagtga gccgtcatcg gtgaggcatg gcagcgcggc
    19021 ttcgatgcga cgcatgagaa acgggacata ggcatcgaag tcgtcgcgat agctcgggct
    19081 atcgacccgc tcgacgtcgt agcgccgtcc accaaatccc gagcgctcgc catccgcgga
    19141 cgctcggact cggatacgct gacgcgactg ctgccgtccg gtgttgaatg gcgggtcgat
    19201 atagatcagg cgcgcgtgtt tgcgcggaag cgtcggaagg acctccaagt tgtcgccctg
    19261 cacgattcgc ctcacgcggc cgagcctatc atcctgcgcc gaccgcgccc atccgcaagg
    19321 cccgcttgca gcaagttggg ccgcgcccta ctctcacggc cgatgcgact gctggtcaac
    19381 ggcgaggaaa agcaggttca accgagcacc acggtcaagc aactgctggc ctcactcgga
    19441 ttggcagaca cattggtagc cgtagagcga aacgaagaag tcgtccccag ggctcgacac
    19501 gagagcacga ctctcaacga cggtgaccgg gtcgaagtag tccatttcgt aggaggtggt
    19561 taggtgtccg aagatgtcct caagatcggc cagttcgagt ttcgttcccg attgttcacc
    19621 gggaccggca agtacccgga cctagagacc gcgagaaacg cgctgcgggc ttcagcctgt
    19681 gaagtcgtca cggtcgcggt gcgtcgcgtg gatttgaagg cgacgggtgc cgactcgatc
    19741 atgacggtgc tccgcgaggg caactatacg cttcttccga acactgctgg ctgttacacc
    19801 gccgaggacg cggtccgtac tgcgcgcttg gcgcgagagc ttctcgatac gccgctcatc
    19861 aagctcgagg tcatcggcga cgagcggact ctgtttccgg acgtccccgc gactttggag
    19921 gctgcgaaga tcctggtcga cgacggtttc acggtcctgc cctatttcaa cgacgatccc
    19981 gtctccgccc ggcgcctgga ggatctcggc tgcgccgccg tcatgccgct cgcggccccg
    20041 atcggcagcg gcctcggcgt acgcaatccg ttcaacatcg agatcatcgt aaagcacgcc
    20101 aacgtcccgg tcatcgtcga tgccggggtc ggaacggcgt cggatgctgc ggtcgcgatg
    20161 gagctcggca tcgacggggt cttgatgaac acggcgatcg ccggagccaa ggacccggtg
    20221 cggatggcgc gcgccatgaa ggcggcagtc gaggcgggtc gcgacgcgtt tctggcgggt
    20281 cgaatccagc gcaagctcta cgccaccgca tcgagcccgc tcgaaggcgt gatcggctcg
    20341 gaccaggacg gtcggaccta ggtcacttcg atgacgcaag ggcttccgcg aatctggatt
    20401 atcaccgacc ccgatcaccc agacggtccg gtcgccccac tccgccgcgc tcttgaaatc
    20461 tgccccccag gcctggttgg ggtccaactt cgtgcgcacg gggtgcccga tcgtcagttg
    20521 atcgagtggg ggctggagct ccgagcgctc acccaagccc agggctgcac cctcgcggtc
    20581 aatcgacgga tagacgtcgc cgagatcgtc ggcgcagatg gggtacatct tccagaatcg
    20641 ggcctgccgc taaaagatgt gcgcgaacat tggcccgcga ttcacctact gggcgtctcc
    20701 cgtcacgact cggtcggctt ggctgcggcc gagcgcggag gtgccagcta cgcttacttg
    20761 agtccggtct ttaccgttcc gggcaaggca gctcccatcg gaatccatgg ttttcgagcc
    20821 gcgatcgctg atgtcgacat tccgacatac gcactcgggg gcatcgaagc agaggatctg
    20881 aaccctctgc tcgccgccgg ggcgtttggg gtggcgattc gacgcgcgat ctacgcgagc
    20941 gacagtcccg aggaatccct gagtcgattc ctcagcgcgc ttgacaaggc cggcgccaac
    21001 ggcgaagagc gagcctagtg ggagcgattc gaactgggct tggatgtctg tcctgggtgg
    21061 gtgctgggct gatcgtcatt gcattgatcc tgaaggcact ctttttcgac atggctgagc
    21121 tcgctcacaa tgggatggcg cccacgctgc tccgcggcga acgggtgctg atacacaaga
    21181 agggcagtcc gacgctcggt tcgatcgccg tctgtcagca tcccagcgag gaaggctggg
    21241 tggtcagccg agtcgctgcg atcgagggca tgaagatcga ctccttcggc aaagagctgc
    21301 gaatcgacgg cgagcccgtc gtattcgatc agcatggaac gacttccttc tacaacgccg
    21361 atacggatgt cacggcgtcc gtcgtttggg gggatgagca cttcggcccc aagccccacc
    21421 tgatcttcat ggaagaggac ggccggcacc ggattcgaga gaccgagcta gcgaccggcg
    21481 aaatctatct gctcaacgac taccgagctc actgggtcca agacagccgg gagttcgggc
    21541 cggtggatgc atcgagctgc agaggcacaa ttgtattccg cctcttcccc gtcgaagggc
    21601 tcgatcggga aattgcgcac cactatttcg agctgattcg atagtgagac ttccgcattg
    21661 cctagagcct cgtccgttct atgctccgcg cgccgaggac aacggaacca ccgatgaaag
    21721 tcgctgtcgt acaatttcca ggctccaacg ccgactggga cgcgcttcac gccgtgcgtg
    21781 acgtcctggg cgccgacgcc tggtatgcct ttcacaaaga gcacggtttg ggagacgccg
    21841 acgcggtcgt aattcccggc ggcttctcct acggagacta cctgcgcgcc ggcgcgattg
    21901 cgggattcag tcctatcgtg agctcgattc gtgagttcgc gggccgcggg gggccggtcc
    21961 tcggcatctg taacggcttt cagatactca cggagatggg gctccttccg ggcgcgttga
    22021 cccggaacgc acatcttcgg ttcgagtgcc gggacatcca tttgcgcgta gaaaccgagg
    22081 gccctttcac cgacatgggc aagggaacga ttcttcggct gccgatcgct cacgccgacg
    22141 gccgttatca caccaccgaa gacggtttgg cggagctcga gcggaacgac caggttgcgt
    22201 ttcgctacac cacccccacc ggcgatccgc caaacggaag cgatgcgaat ccgaacggtt
    22261 ccgtgagtga catcgccgga gtctacaacc gggaaaagaa cgtacttggc atgatgcccc
    22321 accccgagcg agcgagcgaa ggcatcctcg gcaacgagca agggctcacc ctattcacat
    22381 cgcttcgacg gcacttgggg ttcgaatgag cagacccgcg aattttcctg gagagcctga
    22441 cagctcgccc gagatcgccc gcgagttcgg gcttgcgcag catgaatggg atcgaattct
    22501 cagcaccctc tcacgcgcgc caagctatgc ggagctcggg gtgatcagtg tgatgtggtc
    22561 tgagcactgc tcgtacaagt cctcgcgcgt gcaccttgcg cgccttccca cgacgggacc
    22621 acaagtggtt caggggcccg gggagaacgc cggcgccgtg gatattggtg acggattctg
    22681 cgcggtgttc aagatggagt cgcacaacca tccgtcttac atcgagccct accagggcgc
    22741 agcaacgggg gtcggcggca ttcttcgcga tgtattcacc atgggcgcac ggccggtggc
    22801 atccctgaac tcgctacgat tcggccgacc cgaacatcct aagaccccac acctgctcaa
    22861 gggtgtggtc gccggcatcg gcggctacgg caactgcatc ggcgttccga cggttggtgg
    22921 cgaagttcaa ttcgatgcga gctacgacgg gaacatcctg gtcaatgcgt tcacggtcgg
    22981 cattgcgaaa accgacgacc tcttctttgg ggtcgccgaa ggcgtcggaa accccatcat
    23041 ctacgtaggt gcgaagaccg gccgcgacgg aattcacggt gccacgatgg caagcgccga
    23101 gtttggcgaa gagaccgaca gcaaactccc cacggttcag gtcggcgatc cgttcatgga
    23161 aaagcttctg ctcgaagctt gcctcgaaat cttcgccacc gattgcttgt caggcgtgca
    23221 ggacatgggt gccgccggtc tcacgtcctc gtccgtggag atggcagacc gcgcgggcaa
    23281 tggcatcgag ctcgatctcg atgcgatccc acgtcgaacg aaagccttga gcccctacga
    23341 aatgctcctg agcgagtcgc aagagcgcat gctgatggtc gccaaggagg gtcgcgaaca
    23401 ggaggtgttc gagctctgtg ccaagtggga cctggacgca tcgatcgtgg gtcgcgtcac
    23461 cgatacaggg cgcttcgtct gcaaagccac gccggggtat gaccctttcg atgggactgg
    23521 gaagcccgac cccatcgtgg tggtcgacct gcccgtcgat ctattgacca acgccgcccc
    23581 gaagtacgac cgacctcagc aagagcgagc cgagaccgcc tcgttggtga gcatggcagg
    23641 tgatgccccc gacgacctcg gcgacgagct cgttcgcttg atcggctcac ccaacatcgg
    23701 aagccggtct tggatctggc gccagtacga ccacatcgtc cgcgacggca ccgtgattcg
    23761 gccgggcgaa ggcgatgccg cggtgattcg cgtgttctgc gatgacgccg acggcacacg
    23821 ggagaagttc ctcgcgattt ctgcagactg caatggcagg cacgtggagc tcgatccctt
    23881 tgctggagcc gcgatggccg tggccgagtg cactcggaat gtcgtctgcg tcggcgcaaa
    23941 gccgctcggt ctcaccgact gtctcaattt cggaaacccc gaagtgcccg agcagatgtg
    24001 gcgctttgca cgcgcgatcg acggtttggc ggcagcatgt actgcgctcg acgcgcccgt
    24061 cgtctcgggc aacgtgagct tgtacaacga gacggacggc aaggcgatcc ttccgacacc
    24121 gaccgtcgcg gtcgtcggcc agctggagga tccggagcat cgcctcggcc tcgggttctc
    24181 gaacgaaggc gacctcatcg cgcacctcgg tcgtccttcc cgtggagtcc tcggtggctc
    24241 ggagtggctc gtccaacgca ccggatccgt gaccggagca ccggtcggga tcgatctcgg
    24301 cgatgaagcc gcacttcaac gcgcggtcct ggccctcgcg cgcgcgaagc tgctgagtag
    24361 cgcgcacgac gtcagcgacg gaggcttcgc ggtctgcctc gctgaggctt gcatagcgca
    24421 tcggcacggc gcgaaggtca gcctcccttc gaacgaggac gcgcccgcgc acgcgctcat
    24481 gttcagcgaa gaaccatcgc ggatcatcgt cagcctgagc ccaggccacc tgggtgaagt
    24541 cgaagaaatt tgccagcgcc atggcgtgcc cttcgagaag ctggggatcg tcactggtca
    24601 aagcctggtc atcgaaggtg tttgcgacgt ttcgatcgca gcgctcgaga aatcgcactc
    24661 gaccgctctg gacgccatcg taagctagac cgtatgcttc gctacgcttt ggctcggatt
    24721 gattcggccg aggcgatggc gtgaacgacg agccatacga ggcgttcgtc gcacccgtgc
    24781 cgaagtctct attccgcggc ttcgaccgtc gccgccacgg ggcgcttgtc accgcgaagc
    24841 tctacggaga cgagcttgga gacgcccggt tgctccatgg tcacgccgta gagaacgtca
    24901 gcgaactcca tcgtgcgctt ggagtgcgtg atgatgatga attgtgagcg ctgcgtcatc
    24961 tgctgaatcg tttcggcgaa gcggccgatg tttgcctcgt ccagaggcgc gtcgacctca
    25021 tccagcaagc agaaggggct cggcttgtac tggaagatcg cgaagatcat cgctaccgcc
    25081 gtcagagcct tctccccgcc actcatcagc tcgagcgagc ccagtctctt gcccgggggc
    25141 tgtgcgatga tctccacacc ggtttcgagc atgtcgtgag ggtcggtgag ccgcagctcg
    25201 gctttgcccc cgcgaaagag catcgggaat atgcgcttga agcgttcgtt gatcgcggtg
    25261 aacgcctccc ggaacatcgc cctgctctcc cggttcatct gccgaatcgc cctttcgagc
    25321 ttggtgagag cctcctcgag gtcgcgacgc tggctcgtga ggtactcgta acgctcggcg
    25381 ttctcctcgt actcctcgat cgcagtgagg ttgatctcgc ccatgcggtc gatcagccct
    25441 tgcagctctt cggctcgttc ggtgattgca gcgtcgggta cgggtcgtgc gtggtggtca
    25501 ccgatgacat acgcaagctc gatacgatgc ttgtcttgaa cctgttcttc gaggtgctcg
    25561 agcgacatgg tgagctcgcg ctcacgcagt gtcagcgcgt tgagctcggt cgatcgcttg
    25621 tcgatggtcg agcgaagctc cttgagcgca gcctcggacc gcccgagcag cgtctgcgcc
    25681 tcgtcgaagc gcgcacgctg acgggagagc tcctcgcgcg cgaccgaagc gtcgtgagcc
    25741 gtttcgctca ggcgcccccg gtgcagtaga acgcgcccgg ccgcttggcc cagatcgcga
    25801 tccgcgacgt cgacgtcttc gcgcaagcgg tcctctcgcg cgtccagctc ggcgatggag
    25861 cgatcgagtt ggccgatcat cgaacgctcg cggtcggcgc gttcgcgagc ctcggtcgcc
    25921 cgaaccttga cctcggtggc cttcgcggat tgctcgtcga cgacgcggcg cctctcaacc
    25981 agcacgctct cggcgacgcg cgccccttct tgtgcctcgc tcaccagatg ctgcgcgtcc
    26041 gtgagctcac gtcgggtctt gccctcatcg acgtcaacct gcgaaagcga gccccggccc
    26101 tcggattcag ccttggcgat ttcctggcgt cgctcgccga tgcgctgaag ctccaggtct
    26161 acgtggcgca gatcgcgttc agcgccgagg atttccagct cagcgtcgtg ggcctcgacc
    26221 ctgaccgaat caatcgcggc ctgccgttcg gcgatggacg cgcggagctg gccttgcctg
    26281 gccgtcgcgc gctcgagctc ggcgccttcg gccgcagctg cctctcccaa ttcgcggatc
    26341 tcgcgcttga gacgcagaag atgggcggca gactcgtcct tcgcgccccc gaccacggaa
    26401 ccatcgcgta gcaatgcttg gccatctagg gtgacgagct tcccgtggaa cccttggccg
    26461 tgcagccgcg ctgcggtgtc cagcgtgtcg acgaccaaga cgtcgaacaa gaggtagcgc
    26521 acccaggctt catccgcatg ggagtagcgc atcaaggtca acaaaggccc tacgatcccc
    26581 atgcccgtgg gaaactgagg cacgactctc ggtgcgccgc tcggcgaccg cggcaagaac
    26641 gttgctcggc ccttttcctc acgagcgagg taatcggctg ccctcaaagc tgcgcccgtg
    26701 tcgtcgacca gcaagtactc gagcgcccca ccgagcgcag cgcctaaagc ccgcgtgtgc
    26761 tctggcgcgc attcgatgcg atcggcaacc aagcccacga ggccggagcg ctgctcgtgt
    26821 ccagttgcgt tggcgtaccc cgtcagcaca gcctgcgcgc ctgcacccac accttcgaag
    26881 cgttcgtgaa tctcgttgag cgagcgcagt cgcgagctcg tcacggccaa aatctcgcga
    26941 aggcggtcta cctcggagtc ggcctgttgt agcgcctcct tgttggtcga cagagtcgtc
    27001 tcgatctccg actggcgttc gcttgtctcg gatttaccgc ttcgcaaacc caccatccga
    27061 gcttggtgct cgccgcgttg ttgcgaaaac tccacctcgc gagtcgagag tgtctgcagc
    27121 tcgtctcgca agcgttcgag gcgcgtgcgt atctctgcgc gtcgggcctc gtagccgttc
    27181 agcaccgctt cggcaccggc aacgcgggca cgcccctcgg ccagctgcga ccgcgcgttc
    27241 tggagggcca gatcggcctc ttccgcactg tttttgcggg cctgaagctc cgcctcgacc
    27301 tccctcagca gctcgcccgc ctgagtctcg agcgcctcga gctcgctcag cgaaagacgg
    27361 actgtcgacg cctctgcatg caatgcgtct cgttgctctt cgagctgccg cagctcgcgc
    27421 gatgcgattt ccttgcgctc gtgcagcgcg gcttgccgat cctcttgttg acgaatcaga
    27481 ccttcgagat ggcgcaccaa attgtcgagc tcgtacgcgc gcgcctgtcc tcgttcgacg
    27541 tcgaaaccca tctgctgaag caacactcgt tctgcttcga ccgacgcttc ttttgcgacc
    27601 aaggcatggc gactcgcttg gacgtcttcg gtagccttcg acaacgacgc cgagacggat
    27661 cccatttgcc cgcgcagctc caagaagcgg tgagaggcga cccagaggtc caggtcacgc
    27721 atctctttct tgtaccgctt gtagcgttct gccttctgtg cctgacgctt gagcgatgcg
    27781 agactgcgct cgagctctcc gatgatgtcg gtcacacgca gcagattctg cctcgtctga
    27841 tccatcttgc gttcggcggc gcgcttgctc agcttgaact tgctgatgcc cgcggcctct
    27901 tcgatcatcg agcggcgatc ctcgggcttg gaggtgacga tgaatccgat tcgcccctgc
    27961 tcgatgatcg agtaggcgcg tttgcctacg ccggtaccga ggaacaggtt ggtgacgtcc
    28021 aacagccgga cgggggttcg attgatctga taatcgctgt tgccttggcg atcgagtcgt
    28081 cgcgtgacct gaatctcggc gtagtcactg tattcgggcg gggccaggcc atcggtatta
    28141 tcgaatgtga gggtcacctc ggcgaagcca tgaggcccac gattctcgga cccgctgaag
    28201 atcacgtcct ccatgtgctt gccgcgcagg cgcgcagcgg actgctctcc catgcaccac
    28261 ttgatggcat cgacgatgtt ggactttccg cagccgttcg ggcccacgac acaagtgatg
    28321 tcgtggtcga aatgtaccgt cgtctggtcg acgaaggact tgaagccaac gatatggagc
    28381 ttcttgatct tcacgctgcc ccccaaagga gtagatggac gtggctatca ctgaacatac
    28441 gatattgcaa cgctttacgc gaccagaagc gagggtcagg cagtgacccc cactatatgt
    28501 tgtggttgtc tcatgaatcc acgcctaggc gcaaaccagg ctttggtgtc ttacgtggtt
    28561 tcggcagact ttgcctcggc ggccactgcg ggctccgggg cggccttgac ctgcggccga
    28621 ggggcatgac gtcgggctcg gaggaactcg aggaacgcgc ccgccatctc caggtcgcga
    28681 accgcgagcc cgtccaccag ctcatgaagc ctggtccgcc ctgtctccgc cggcaggggg
    28741 agctcgttcg ctgaccctag gtcgggcgtt ccctccgggg ccaccgcggg tgcgtcgggt
    28801 tcgggatgga cctcgtcggc cgtgggaacg tgccacgtcg gccgctccac cgagtgaatc
    28861 ccgtgggcgg tgagccagga ctccatgcac gagcgcagcc gttcggagcg gaacgcgaac
    28921 cagcgctccc tgtccacggg gtgactcatc aaggcatcct tgaaacgccg aaacgcaccc
    28981 ttgccatcaa tcgcgatgtt caaacgtcgg cgcagctcct cgtcttcgac cgtggcgatg
    29041 aagcgctcca tccatcggta ttgctcgcgg gacgaaacag ggtcgatccg cagataccgt
    29101 ttgtcgttca tgatccgggt atgcatcgaa gggtcagcaa ccccatcgac gatgcgaatc
    29161 acctcgccgg tatcgaagtg tagataactg tgcacttcat gtgcattgtt ctcaaaagcg
    29221 tcttcgagtg cctcccaagc tacgggtacg tctaatgctt ccttgtcttc tatgtcttct
    29281 atgtcttgcc cgacatcatt ctcgttcatt ttattcccat ctcacttcgc gcccgccgga
    29341 ccgcgtcgcg ccatcgcctc aagtgcccgt ctcgttcggg ctgggttacc tggggctcaa
    29401 agctacgttc gatacggtaa gcatctatta cggaaccaag accgtcgaat acacctacgg
    29461 ccatgccagc taagtaagca gcacctaagg ctgtggtttc gacattctcg ggacggtcga
    29521 ccgtcacccc gagcatgtcg gcttcaaatt gcatcaataa gtcgttccgg gccgcgcctc
    29581 cgtcgaccct gagtcttcgt atgggtcgct tggcgtccgc ttgcatggcg tctaccaaat
    29641 ccacgatctg aaaagcgatt ccttcgaggg tcgcgcgcgc caaatgggct gcggtggtat
    29701 ctctcgtcag cccgtagatc agcccgcgcg cacggggatc ccagtacggc gccccgaggc
    29761 ctgctagcgc tggcacgaac acgacgtcgc ccgtcgaatc gacctcccgg gccttggctt
    29821 cgatctcgtc cgaagaagcg atcaggccca gtccgtcgcg gagccattga accgcggcgc
    29881 ccgctatgaa cgcgcttcct tccagcgcgt agaccgtctg gtcaccgagg cgccaggcga
    29941 tcgtagtgag cagtccgttg tccgagtgga ccggttcgct cccggtattc atcagcagga
    30001 atgcgccggt gccgtaggta catttcgcgt cgccaacttc aaagcacgtc tgcccgaaca
    30061 atgccgcctg ttggtcccct gccatccctg cgattggaat tccctcgggc agccacggca
    30121 cgcccgacgt caccgcatac acctcggagc agcttcgaac ctcgggaagc aaggccatgg
    30181 gcacgtgaag ctcggcggcg agctcttcat cccaacgtcc tttgacgata tcgtagagca
    30241 gcgtgcgcga agcattgctc gcgtcggtga cgtggacttt gccacccgac aaacgataga
    30301 ccaaccagga gtcgatcgtg ccgaaggcca gctcacccgc ctcggccctg gtccgcacgc
    30361 catcgacgtt gtcgaggagc cattcaacct tggtgccggc caagtagggg tccagcacca
    30421 ggccggtccg ctgacggaac atctcttcct tgcccgcgtt cttgagctcc cgacagcggt
    30481 cggccgttcg tcgatcttgc cagacgatgg ccttgtggac ggccttgccg ctctttcgat
    30541 cccagagcac ggtggtttcg cgttggttcg tgatccctat gccgaggcaa tccctcgccg
    30601 aaaccccggc ccgcgcgatg gcttgcccca cggcgtcacc gacgctcttc caaatttcat
    30661 cgaggtcgtg ctcaacccag cccggccgcg gaaaatgctg tggaaattca caattggcgc
    30721 gagccaagat ctccccgcga ggagacaaga ggagcgcggt ggaacccgtg gttccctgat
    30781 cgatcgacaa aaggaagccc gacatgcgaa cgggtacggt acgaagcctc gggggcaatg
    30841 taaagcggct actctcagtc gctcaaccag gcccgaaacc gcggccaggc ctcgcctaga
    30901 tgctcttcga gctcggtagt catcgagtcg atcgacgaat cggctgcacc aagctcgcgc
    30961 gcgcgctcgg cgcaaaccat cgcagcgagc cactgctccc gcgcgctgag cgatcgggcc
    31021 agaagcggca tgctcaggtg ctcgctcgcc cctaagcgga tcgctcgccg gagaagtccg
    31081 atggcctcgc catgcttttc ggcggcaacc gacgcgcgtc caagcgcgag aaacaagtcc
    31141 cccgcttcgc tggactcccc ggcccattgc accccgagtc gcagtacttc gtttgctgcg
    31201 tcgagctcgc cgagctcggc gtaggccgtt cccagcaaat ccaggctggt cgacaagcgc
    31261 gcgcggacct ccttgctcaa gccctccgcc tcccccgtgc gaaggagcat cgagatcggt
    31321 ccgagccacg gattgagcaa ggcgacggtc gcgctccagt tcccgcgctc gttggcctct
    31381 tccgctcgat ccacgacatt gaggtcgatc gaaacctcct cgcgacggtg gagtccaagc
    31441 tcggagcgca ctcgctcgtc gagaatgtcc cttagatcat cgagggtgag cacgtgtcga
    31501 gctccgttga actcaatcgt gacctccgag agaccgagct cgtgtcgcaa ctcgaggggc
    31561 tgtacctgga acagcacggc ctcgagcatg tatcgcccac cgatgtgcat ctgaagcgcc
    31621 tcgacgccgg ggtcgtcagg caggccctcg ccttggagcg gcacgagcag gtactgtccg
    31681 atcgtctgac ggaactcgga aagtgggagc tggcggacgg tcgggtcctg cccgatcgag
    31741 aactcgaccg tgctgaggtc ctgcgattga ggattcgccg tgatgctgag cacccgggcg
    31801 cccgcaatca acgcgagcgc gagggtactg cggccgatga tctggcaggc cttgtcgaag
    31861 tcgtccgact gctcgagcac tgcctcgaac caaccgtccg gacgcgctgc agccaggtcg
    31921 tagacccgct gagaaccgcc cgtcacgtca ggcctcgccg ctaagcttgc gccggtacca
    31981 ctcgatcgtg tcgagcaggc cctgctcgag ctcgacgtga gcgcggaaac ccaggagctg
    32041 ctcggccttc ttcacgtccg ggatgcgaag ctccacgtcc gggaaatccc agtcgacgaa
    32101 ctcaatcgga gatttgctct ccgtcagacg aacaatcagc tgcgcgagct ggtagatggt
    32161 gatcgtactt cgcgggttgc cgatgttgaa gctctcgcca atggcttctt cgcgttccat
    32221 ggcgagcaag atcgcgttga cgatgtcgtc gatgtaacac caggagcgaa tctgatcgcc
    32281 ttcgttgtga attaagatct tttcgccttg cagcgcgcgc accacgaatg cgtggacggc
    32341 accctctccg acctgcccag gtccgtagat gttgaaaggt cgaatggaga cggtggggag
    32401 gccgaactgc ttccagtagt tgtgcgcaag gtgctcggtc gcgagcttgc tgaccgcata
    32461 cgtccatcgg gcttcgccta cggcgcccag gctggtgaca tccgcctcgc ggacgcgaaa
    32521 cgcgtaggat ccaaaaacct cactcgtcga aaagtcgatt acacgcttca ccccggtgca
    32581 gtcgttcgct gcacggagaa cgttcatcgt accctcgaga gagatctcca tggtcatgac
    32641 gggattcttt aagaccgtgt ccacgccggc gatcgaagcc atgtgaatga cgtactcgca
    32701 gccggtcacg gcccggcgga cggcggtcat gtcgcgaacg tccccaacca cgagctcgac
    32761 gttcggatgg cgatccaggc ccgccttgga gagcgcgttc cggcgcagga tgtcgaggat
    32821 gcgaatatgg ttgtctttgg ccagacgcgc ggtgagcgcg gtcccgatga accctgcccc
    32881 gcccgtaatc aggacgtgtg cccccttcaa catgaccccg agctagcaca atcggaactc
    32941 ggaagtcgag aaagctcagt ccctttacag ggggctcccc gcatgggatc gtgttgaaac
    33001 gagggggaca tgggcatctt cagtcggctc aaccgggtga tcaaatcgaa cctcaacgct
    33061 ctgatcgacc aggcggagga cccggacaaa atgatcggcc agaccgtggc cgacatgaaa
    33121 gcggcgctca agcgcgcccg caaggacctc atcgaggcga tgggaacggt caagcggctg
    33181 gaaagcaagg aacgcgagct cgacaaggag gcagccgaat gggaacaaaa ggcgatcctc
    33241 gcgctcgagc gggacgatga tgacctggcc cgcgaggcgc ttcgccgaaa ggcgcgtacc
    33301 ctcagcgagg ccgagaaggt tcgggctcga gccgcggagc agtcgactgc agcggaggcc
    33361 atgcgagcgc agctcgaacg gatcgaggag aagctcgacg acctcaagtc gaggcagaag
    33421 accctcgccg ctcaggtgcg acaggcgcgc aatcagaatc cagacccggg acaacccacg
    33481 ggcgaaagac ttggaggagg tgcgttttcc gacctcgacc gaatggccga ccagatcgac
    33541 caactcgatg cggaagtaga ggctcatcag ctcctcgatg accctaggcg ggccgagctg
    33601 gacgcccgat tacgtgcact cgaaaccgat gaaggagacg acgctgtaga cgacgagctc
    33661 gccgccctca aagcaaagct gaacggctga cggcgcagcg cgatggaacg tttcgtcctc
    33721 tggcacagaa tcctcccgcc cgccttcgaa gacgaggaga acacggctgc ggccggccaa
    33781 tgggcccggt acgtcacgaa ccaggtgcag aacggcggag gagaggtcat ctcctctctt
    33841 gcgggcacgg tggtcgcgag cttcgagctg cacgggctca agaccgcaat caacctggca
    33901 ctgcggctgc tcacggaagc cgaatcgcaa cccgtacccg caggcggtct gcccatcgcg
    33961 ttcggcatcg ccacgggcga cgtccaacgg gtggatgacg atacaggccg gttgacgaac
    34021 agcggaagcg cgatcgatcg ggcccaattg ctcgccaacc aggcgcgcgc cggcgaggtg
    34081 gttctcgatg tcgagtctcg cgaagccgct tcgcgcacat acctcttcgg ccgctcggtc
    34141 agcactagct cgttcgcgct tcacggcgaa gcgatcgacc gaagccggcc gttgctggac
    34201 gagtgccggc ggtcggtcgg tctcgtccac gcggctccca ttcccgcgag cattcgtcgc
    34261 gcgctcgagc cactcgagaa ggctgcggcc ggatcagaga caatcgcctt ctatctagag
    34321 gggcctcttg gaattggggc gcgcaacggg gtttttgcgc tgcgggacga gcttcgaccg
    34381 gcggtcttcc tcgagattgg cgccgttcct ggcgggctcg agcctctcgg cgggcttcgc
    34441 ctggccttgc tcaaggcatg gcggtcgctc gaacccacgc gccaggcact gaagaaggtc
    34501 gcgcccggag ttgccgacgc ggtcaagtcc atcgcttgtg ccacacctcc tcagcgcgaa
    34561 gcggcggtcc attctctcaa ggtcatgttc cgcgctcttc ggcaagagca tgcgctgcct
    34621 tgggtttaca tcgaccgcat ctcagcggtg gatccggcca cgctcgatgt cttgggcgct
    34681 gcgctctccg acgagacagt cgtcgccgtg cttttggtgc gaacgaccga gtcgagcgtg
    34741 cccgctggcc ttcgtggcct gaagcgaagc acgcgaatcg agcttcccgc gctcgagcca
    34801 agcgaagccc tcatcgtggc tgctggagtg ctcggaacgg accccgaccc ggagatcgcg
    34861 aagcaactcg tgatcacagg agggaccacg gtgctcggca tcactgaagc ggcgcgcacc
    34921 atgatcgcca caggcgacat cgtgcatgaa ggcggcaggt tcgtttggcg cgaagcagat
    34981 gtcgagcggc gccaatacct cgggccgcgc gcgcttctcg aggagcgctt cgccaccctg
    35041 gacacctcgt cgatgcgttt tctggaggtc gcatgcgccg ctcttcctgc ctcttccgcg
    35101 caggtcgtag acactgcagt cgcgttggac ggaattccgg atgagtcgcg ccatcgcgcg
    35161 ctcgaagcgc tggtcaacga cggtctcttg acgcctcagg ccaagccaca gtcggagtta
    35221 ctccgccgtg caatcgttca acggatgccg cctgcacgac gcacggaagt ctatcgtttc
    35281 gtagcgcagg ccctgcacac cgccgagccg ctgctcggcc cctcgatggc ggccaccgtc
    35341 ggcgcgtacc tctgcgaggg cggcgacctc gttcgcggcg cgcacgcact cctcgaagcc
    35401 ggctcgctcg cggctgacaa cggatacacg agcgccgcgg tccgtttggc cgctgccgcg
    35461 gttcagtacc aacccgatac cgaaactcgc gagggcgcat ccgagatcag ccgcgcggta
    35521 cgcctaccat cccgaccgcc cgaggacgaa gaagaggacc gcccgaccat ccccgtcccg
    35581 gaagaagaac ttcgaaatcc tacggaagag atcattcgag tgctccgcga tcgtgaccac
    35641 gagaagttcg atgcattgat cgcgtccgcg atcgcttcag gtggcgacct caccggcgca
    35701 cactgccttc gcgtgatctc gtatgtcatc cgcgacgact tgcccgcggc ccgggaagcg
    35761 ctggctgcgg ccaagaagag ccccaatcca actccgggcg cacccatccg aatcgcgatc
    35821 gcttcggcct gcatacaact ctctcggggt cgaaggcgcg acgccatgat cgatgcgctc
    35881 gaagcgctga gcacagctcg ctcccaccag gacggacctg gagaagcggc ggcgctcaaa
    35941 atggtctctg cgacttgcct cgccgcaggg ctcgagcgcg atgccactcg cttggcgcac
    36001 gtggcagaag ccgtggctag ctgactccgg cgcggcggat cgggtacgag ccgaggaccg
    36061 tcagaaaagt cgtcaccgtt tcgaggtcgt gaagagcggc ctgtgtcttc tcacgctcga
    36121 cgttgccttc aaaatccacg tagaagatgt actcgaacgg tgtgtcgggc ttcggtcgcg
    36181 actcgatctt tgtgaggttc aaaccctgtt cggcaaagat cgagatgcag cgcgccaagg
    36241 cgccctgttc gtggatggtc gagaagatca acgaggtctt gcacgggaca ccgtgctcgt
    36301 agtgtgccgg ctccggagcc acgatcacca tgcgggtgta gttctccggg tggtcggcaa
    36361 tgtcgcggcg caagataggc aagccatgga gggcggccgc ctgttcgctt gcgatggcgg
    36421 cctgagtcgg gtccgcatcg tgcttcacct tctcgaccga catggccgta tcgacgaacg
    36481 cctccacatg acagtcctcc aaggtctcca agaactcgcg acattgagcc agtgccaccg
    36541 gatgggaata gacgcggcga atctcgctga cgggcacatc ggcggcaccg atcaggcaat
    36601 gggcgatggg ctggaactcc tctccgacca gggacagccc catcctcgcg aggaggtcgt
    36661 agctctcgtt gatcgaaccg gcgatcgagt tctcgatggg gagcacggcg tagtcgatct
    36721 ctctggcttt cagtgactcg agcatctcgc ggaagctgtg gtacccgacg cagcgtaccg
    36781 aacccgcagc gcccgaaaag tgccgcagcg ccgcgagctg gctgtacgcg ccctccgtgc
    36841 cctggtaacc gaccaggagc gtctctttgg tctggttcat cgccaccgtc gtcagtcccg
    36901 aaagttcacg aactgtaggg ggagatcgaa gtcctgttct ttcaaaaacg caatcgtgtc
    36961 ctgcaggtcg tcgcggttct tgcccgaaac ccgaaccgag tcgccctgaa tcgaagcttg
    37021 gaccttcaac ttgctgtcct tgatggcttt gacgatctct ttggccttct ccttggagac
    37081 gccctctttg agcttgatca actgccgcca catctgcccc ccggcctgtt gcggtttctg
    37141 cggatcgagt gacttcagcg agacctggcg gcggaccatc ttggtttcga gcacgtcgcg
    37201 cgcagcatcg agccggcctt cgctgttgga acgaatcacg atcccttctt cggtttgttc
    37261 gatcgtgctt tcggttccct tgaagtcgta tcgctgcgcg agctctttgc tcgcttggtt
    37321 gcgcgcattg tcgacttcgt gttggtcgat cttggaaacg acatcaaacg acggcatctt
    37381 gacctccgag gccccacctt atcacggcgc gagctttcgg cgacccggct tctgcgagtc
    37441 cccatcggag tcgcccttca cgagcggcga aaagaagttg tccttcttgg tgtactcgtc
    37501 gacgcgaaac gcgaacttgt agctcggagc cgatcgcatg ttgcagtcgg ttcgttcgca
    37561 gaatctgcaa gtcgtcccga ccggcaccgc catcttcttg gggtctgcaa agggtaaatc
    37621 atcggcatag gcgaagtgct tggcgtcgtc cgcgtgggtg cctaggccga tgctgtaggt
    37681 ggtacctttc acgagcgagc cctgctgagg ctcactcacg atcttggcaa agcagaaata
    37741 ggtcgagcca tcgggaaacg ccgagtactg cttggtgatc acgctcgggg tgagaaatgc
    37801 cagatgcacg gccatcttgg gacaggagcc gtcctgatgg ggaaatcgaa tgccatcccc
    37861 cgaatagcgc ttggagatgt ttcccgcgac atcgacccga aggaagtgca tcggagcccc
    37921 gcgacgccga gggtccgaaa ggttgcacat cctgtgcgca accgtttcgt aggacgattc
    37981 gaagagctgc gcgagaagct cgacgtcgta ccgcgagctg gtgacctctc gatagaactc
    38041 cggataagga agaagaagcg ctccagcgaa gtagttggcc aggtgaatct tcagcagttt
    38101 tcgcgtctcc ccgtgttgac cgctgtagcc ttcgaggatg ggttcgtgaa gcttctcttc
    38161 gtcgaaaaga ccgagcgcga cggtgtgtgc caattgaaac ttcagacgtt gttcgaagag
    38221 atcgctcgag acggtgagca cccgctcttc ggcgttccac gttcggatga cggagctgtc
    38281 ggtgcgctct ggaattcgat gaacgcggac gcctacctgc tcgagaaggc cacgctctag
    38341 gtcatcgctg gtcacgcgtc gtccgaatgc gcaatcacgg cgaaactgct gcgcgcgctc
    38401 ttcgagctgt tcgaaccagt tgtcgtgctg ctcgaggaaa tccgtcacct cgtcgtacgg
    38461 gctgtagtcg aagcgaagcg aaccgtctcc cgcctggcgt tcgcgttggg caacactcgc
    38521 catgacgtgg tcgagctggc tgcgtgtgtt cttgtacaag ttgaacagcg ccgtgatcgt
    38581 cgtgatcatt cgcggttcgg cgctgatgct cgcgaggtcc tcttcgctca ggttgagcga
    38641 gcgaagcaac ggctcgtcga gaagcttcgc gaggccatcg tcgacccgtc gttctccgag
    38701 gctcgacatg aagtcttcga cctgcacgtc gtagagctcc agggcctgaa acaagagagg
    38761 caactgaacg acacgtttcc ccttctcgat caggctcaga taggcggggg agatcttgag
    38821 acggcgcgcg gcctcgctct gctgcaggcc ccgccgcagt cgaagctccc tcagacgctg
    38881 ccccatcgcg gcgttcgtct cggccatcag agtcgtttac cgcaaattta caaaatccac
    38941 cagggtccag aatgggcaaa aagcgctcgc cggcgggcgc ttgaccgatc ctggccgtgt
    39001 gataatcgaa gagagatcgg gtttgaccgt gtgaagctgt aaagagcacc gcaacggtcg
    39061 caagagccca gaaggcaagc gagctcgaaa acaagctagg cttctgcccc cttgggcgaa
    39121 tcgcagacga aaggaccggc gaatggcgga gtacggcaag cgggagcgcg acagaccgtg
    39181 gatcatgcga acctactcgg ggcacagcac ggcggccgca tcgaacgccc tctatcgcac
    39241 caacctcgcc aaggatcaga ccgggctgag cgtagcgttc gatctgccaa cacagactgg
    39301 ctacgacgct gacgatccgc tcgcccgtgg tgaggttggc aaagtcggcg tcccggtttc
    39361 acacgtcggg gacatgcgcg ctctgttcga tggcttgcca ctcgatcaga tgaacacttc
    39421 gatgacgatc aacgcgacgg cggtctggtt gctgtcgctg tacatcgccg tcgccgagga
    39481 ccagggcgtc gaccatcacg ttctgcgcgg caccactcag aacgacatca ttaaggagta
    39541 cttgtcgcgg ggcacgcaca tctttccccc ggcggcctcc atgcg
//
LOCUS       DASRZF010000396         2315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4427890, whole genome shotgun sequence.
ACCESSION   DASRZF010000396 DASRZF010000000
VERSION     DASRZF010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4427890"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VFG22_17585"
     CDS             <1..229
                     /locus_tag="VFG22_17585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418112.1"
                     /translation="RAAREFLKSREPDQSDMTMVAIARSALVEQGIPEEKHTPELVED
                     VLKLIEKRASLQADPRDSGGMIPPDQIQWAR"
     gene            439..2253
                     /locus_tag="VFG22_17590"
     CDS             439..2253
                     /locus_tag="VFG22_17590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418113.1"
                     /translation="MREARLPKPRDLSTEIPAATNTFGKITESVGQDLPLIAASSVAM
                     PVLAPAAPLLAMLGEIGLGVGASAAAAAAREAGASDATQIGTELLASAVVPGAVTSRG
                     PAVAKRIARHASQYDDVARLAAKHGVELSPEIFDRAASELKRRMLLDPVDPNRRIREA
                     SEVLGRDIEAFGGATGEGITPTLSQSVGELGGRNIIDLEADMARKDKDFASRTQGRVM
                     HVQDKLDADVTAMRPQGTLGQVEAAALKRFDDMEAETQSLWRAVPKDELPLVSTGKLN
                     ETIDDIIERVGVGYEGDLPREVKLIRDAGPEATIDELIRWRSRLLRRNRQANQFGASQ
                     VLQDEAAAGAPLIHALTEEIHAVGDTGGGQLRAALASTRRNKELFDSSTNPLWRELGQ
                     KGQRDPHKIVGAINRSVDPVESARQATSMLSESEEGLTGLRAVFFDDLMDGRTPRQIQ
                     KDLSNPLRRETMTEIFGEQGMTFLDEVARRSEVVARRTGAERGAAFRTGSNVGEAAQQ
                     LSPSSSANSLLWALSKKAQTWMRDKALNNQEIAHVMLIATEDIRVAKALLDLPTPRAE
                     AAWRIIMEQTVRNAKGKAAREAARAKLRSAPDRSEPGK"
BASE COUNT          516 a          632 c          758 g          409 t
ORIGIN      
        1 cagggcggct agagagttcc ttaaaagccg ggagcctgac cagagcgata tgacgatggt
       61 cgccatcgct cgttcggcat tggtcgaaca gggcatccca gaggagaagc acacccccga
      121 gctcgttgag gacgtgctca aactgatcga gaagcgggca agccttcagg ccgacccgcg
      181 tgatagcggt gggatgattc cacccgatca gatccagtgg gctcgataat gccgaccaca
      241 gaagaaatag ccaagcagat caagcgtcgt gcgcgactcc agcggaagcc gcctgcaccc
      301 ggcgtattgg actcgcttat gcgtggcatg tcgcagtccg gtttcttcga acggcaagag
      361 gcagaacccg gttcagtcga gccgaatccg aatttctcgg caccccgtaa ttggatgcct
      421 gaagagatgt atacggcaat gcgtgaagcc aggttgccga agccgcgcga tctgtcaact
      481 gagatcccgg cagcgacgaa cacgtttgga aagatcaccg agagcgtcgg gcaggatctt
      541 ccgttgattg ccgcgtcttc tgtggcgatg ccggtacttg cccctgcggc gcctttgctg
      601 gcgatgctcg gtgaaatcgg actcggagtc ggcgcctcgg cggcggcggc ggcggcccgt
      661 gaagccgggg cgagtgatgc gacacagatc ggtacggaat tgttggcgtc tgcggttgta
      721 cccggcgcgg tgacgagtcg cgggccagcc gttgcgaagc ggatcgcgcg gcacgctagc
      781 caatatgatg acgtggcacg actggctgcg aagcatggcg tcgagttgtc gcccgagata
      841 ttcgaccgcg cggcaagcga attgaagaga agaatgcttc tcgatccggt tgatccgaat
      901 cgacgaatcc gagaagcgtc tgaggtcttg ggtcgagata tcgaggcgtt cggtggcgca
      961 accggcgagg ggattacccc gactctgtct caatcggttg gcgagctcgg cggtcggaac
     1021 atcatcgacc tcgaggccga catggctcga aaagacaaag acttcgcgtc tcgcacgcag
     1081 ggccgcgtga tgcacgtgca ggacaagctc gatgcagacg tgactgcgat gagaccgcag
     1141 gggacgctcg gacaggtcga agcagccgct ctcaaacgat tcgacgacat ggaggccgag
     1201 actcaatcac tttggcgagc ggttcctaag gatgagcttc cgctcgtgtc gacgggcaag
     1261 ttgaacgaga cgatcgacga catcatcgaa cgagtgggcg tcggatacga gggcgacctg
     1321 cctcgcgagg tcaagctcat ccgggacgca ggaccagagg ccacgatcga tgagctgatc
     1381 cggtggcgtt cgaggctact ccgaaggaat cgtcaggcca atcaattcgg cgcgagccag
     1441 gtgcttcagg atgaggctgc ggctggggct ccgttgattc atgcgctgac cgaagagatt
     1501 cacgcggtag gcgacactgg aggtggtcag cttcgcgccg cattggcgag tacgcgccga
     1561 aacaaagaac ttttcgattc gtcgacgaat ccgctctgga gagagcttgg acagaagggt
     1621 caacgagacc cgcacaagat cgtgggcgcc atcaatcggt ccgtggatcc ggtcgagtcg
     1681 gctcgtcagg cgacttcgat gctgtcggaa tcggaagagg gactcacggg cctgcgtgcc
     1741 gtgttcttcg atgacctgat ggatggccgg acacctcgac agattcagaa ggacttgagc
     1801 aatccgcttc gccgtgaaac gatgaccgag atattcggcg agcaggggat gacattcctt
     1861 gatgaagtcg cacgccgttc cgaggtcgtg gcacgtcgaa ctggcgcaga gcgaggggcc
     1921 gcgttccgca cgggatcgaa tgtgggagag gccgcgcaac aactttcgcc gtcctcgtcc
     1981 gcaaactcgc tgctatgggc actcagcaaa aaggcgcaaa cttggatgcg cgataaggct
     2041 ctgaacaatc aagagatcgc gcacgtcatg ttgattgcca cggaggatat ccgcgtggca
     2101 aaggcgttgc tcgatctacc gacgccaaga gcggaagccg cttggcggat catcatggaa
     2161 cagaccgtca ggaatgcgaa aggcaaagca gctcgagagg ccgctcgcgc caagcttcgt
     2221 tctgcccccg atagaagtga accgggcaag tgatctaacc gccacccagc gggcttagga
     2281 gagagacgat gaagacactg agaattctga tcgca
//
LOCUS       DASRZF010000397        16311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3397744, whole genome shotgun sequence.
ACCESSION   DASRZF010000397 DASRZF010000000
VERSION     DASRZF010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 16311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16311
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3397744"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /locus_tag="VFG22_17595"
     CDS             complement(<1..1049)
                     /locus_tag="VFG22_17595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418114.1"
                     /translation="MGAFPLYSLDAYGHLAQGRQIAQLGRVPELDPFSFWKPSPAPWS
                     NYEWGYDLLSWLLYDRFGPNVLIGIKCILLAVLGYVLVLLAARLAKGAALAAPLAATV
                     AILFAPLARIRFTVRPQIIGLVFPAVLLLGIHALYSERLSSRAKLGLVFALGAMQLAW
                     VNMHGSHLLGLLITALFLLFSIRTGAFTSMLSLLAVQTVATFCTPFGIDIVTDAISHV
                     FRPEYRELVTEWAPWSPGHPLYLLLGPMAAAILLLVAMRPVVRGSRYGMAYGVFCVLL
                     SLMAFRSIRFVAHQLLLTAPFIGAGFAQLEWIRSARRTVAVALALAFAGALWVAPRLE
                     PFVPFGFGEPRLGHP"
     gene            complement(1146..1976)
                     /locus_tag="VFG22_17600"
     CDS             complement(1146..1976)
                     /locus_tag="VFG22_17600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418115.1"
                     /translation="MPWGILFLAAALLAGCEDDAALARTREQLREVDYPRVQALIQED
                     LQKHQAGVLEAAEKLGPGFALSDPVARESQVRAALRILQQPKKGIDEFVASPMSFLAA
                     IGIDGRVIARDREADRMKGQDFKERFEVVRKALGGAAATGLGEFYAEDPSAPSSWSIL
                     FAAPSVRDGQVLGVVLAGIPLSRLAQRLSRQFRVEQAEGAPVWVYLYKGNRLFYWDTP
                     PEVDALVRDPGARAKGLASSPAGYTEKVRLQGALEVYGVFPLGLLGPDVGTIIFRTPE
                     "
     gene            2110..2688
                     /locus_tag="VFG22_17605"
     CDS             2110..2688
                     /locus_tag="VFG22_17605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418116.1"
                     /translation="MSQPILQTSSPTSRPESITAVELHMMPRDEVLALYHQLEAPAFE
                     EMHGEFAASLLDQGHALGYLLSAFAVNAKGRWLCKAFEPSGPNEGHGYNSFMTPRGVR
                     RSARMRTHIGPSKLPGDTKDAFHLEYADFNDFQRGGPGGALLHTMFDEVRKVRAGLYL
                     GIGRVGFTKKARSEPHPFILEGPVARFMSARL"
     gene            complement(2701..3867)
                     /gene="moeB"
                     /locus_tag="VFG22_17610"
     CDS             complement(2701..3867)
                     /gene="moeB"
                     /locus_tag="VFG22_17610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HET6418117.1"
                     /translation="MAKTYADMLQEVKGAIRQVSLEDLKSRLDAREELILVDVREKDE
                     WRQGHVPGALHIPRGFLEMQSGSRLPDKDATIVTICAAGIRSAFAAKVLQDLGYNNVE
                     SANPGFNQWKDQGFPTTQPFAFTDAQLNRYSRHLLLPEVGEQGQAKLLQGRVLLLGAG
                     GLGSPAALYLAAAGIGTIGLVDGDTVDESNLQRQVLHGLDRVGESKVMSGKRTIENLN
                     PDVKVIPFNERLTRQNVDRIFDQDWDVIVDGLDNFATRYLVNDASVWKDIPVVHGSIF
                     RFDGQVTTFWPHHGPCYRCLYPEPPPPHLAPSCAEAGVLGVLPGAIGTLQATEAVKIV
                     LERGDPLVGRLLQYDSLQMQFRTFKLRRNKDCPVCGDHPTITEYIDYEHFCSGG"
     gene            complement(3979..4497)
                     /locus_tag="VFG22_17615"
     CDS             complement(3979..4497)
                     /locus_tag="VFG22_17615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6418118.1"
                     /translation="MQTKEQYNEEILPCVPELTRSALRLARGTALADDLVQETLARAW
                     QARISFRPGTNARAWTQRILFNTYINHYRKRKREKEALEELCRLACSSDRSSSEVLSD
                     EVLAALTQLRPEFREAVELVDIAELSYQDAAFRLECPVGTVMSRLHRGRKRLKSVLED
                     YAREEGILSSAA"
     gene            complement(4641..6149)
                     /locus_tag="VFG22_17620"
     CDS             complement(4641..6149)
                     /locus_tag="VFG22_17620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HET6418119.1"
                     /translation="MQQLFTRDESRDVDRGAMDELGVASLVLMENAGRGATDLICRSF
                     GDRLGRVVLIGGPGQNGGDAWVVARRLSLLGHEPLAVLLGDASRLRGDARANWALLGK
                     LGVESLEVAPENAESIEGTLADATLIVDGLFGTGLDRPVTGGYADAILAMNRASAPLV
                     ALDLPSGLDADTGAILGVAAQAALTVTFAAQKRGLHQYPGVQHAGEVRVADIGIRVIQ
                     APAASLWTGDDLPALIARRAPDAHKGTNGHLLVIGGAPGKTGAALLAGRAAFRAGAGL
                     VTIGARADSRAALEEKVIELMTSSLPEHVDQSAVEALCEGMRAVVLGPGMGLDELGRS
                     WAHCVALHAPVPSIIDADGLTNLGSEGLAALKGAAAPRILTPHPGEAARLLGRSSADV
                     QADRYAAAAALAEESGQTVILKGARSIVAGSGALRVCGEGTPALGVAGTGDVLSGVVG
                     ALAMSLAPLDAAIAGALLHALAGIAASTGDRGLIASELADAVPAVLAEQLGY"
     gene            6199..6666
                     /locus_tag="VFG22_17625"
     CDS             6199..6666
                     /locus_tag="VFG22_17625"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HET6418120.1"
                     /translation="MNAKHPSESFTEMTELVLPQHTNAIGTAFGGVIMSWIDICSSIA
                     AKRHCGNVCVTARVDALEFRAPIKVGDVVRLTARLNGAFTSSMEIGVRVEREHGRTSQ
                     RTLCADARATFVNLGEDGQPCPVPPLLAASEEDRQLAAQAAARRDQRRLGRNV"
     gene            6684..8543
                     /gene="ppk2"
                     /locus_tag="VFG22_17630"
     CDS             6684..8543
                     /gene="ppk2"
                     /locus_tag="VFG22_17630"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HET6418121.1"
                     /translation="MPILPRAQLFEFNDRDWVPESLRDTIVETLSRSLDWGGFLAPLA
                     PVIDEFLSAAGTTEVLDLCAGAGGPALILSQEAERLGLDAPRYLMTDLYPRVPLWTEA
                     TRRRPRSIDFIEDPVDATRIPASISRGRARVMVNAFHHFQPEMAGAILRDAVEQEASI
                     LISESFERDPRGAFPMIPIGIPALLATPLLSPRNRLAKAAWTWLTPIALAAGAWDAII
                     STLRIYSESDLRDLVAPFGHGYRWKYGTYDYPFGGRGCYFQGVPAAAQPVSPGKPADG
                     LRTFPRKPNGPSALTDRMTMDDEKTYQDTDGQRPGDGDATIAREANARRAELAAELKQ
                     EVDALEEPSGPQTDARTAEAMRKKALRRYLEEQSLRPYQAELIKMQRYLEESGTRMII
                     LFEGRDAAGKGGTIRRVTRYMNEKHYRVVALGKPTERERTEWFFQKYVQHFPRAGEVV
                     LFDRSWYNRAMVEPVFGFCTDREYKNFMRGVTGFEKDLVRQGTVLIKLYFSVTKDEQN
                     RRFERRKADPLRQWKLSEVDVQAQERWDDFTNVKYEMLKRTHTTHAPWKIIRSNDKHA
                     ARLNALKLILNSVPYERLDESLDFVPSSEIVISGARELEKMEAQRLRSGKFLV"
     gene            complement(8591..10840)
                     /locus_tag="VFG22_17635"
     CDS             complement(8591..10840)
                     /locus_tag="VFG22_17635"
                     /inference="COORDINATES: protein motif:HMM:NF018504.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase PrkA"
                     /protein_id="HET6418122.1"
                     /translation="MSEGGLKTALETIRGEVKARFEAQKRVLSYGEYLELVQDNPRRY
                     TRDAGRYLKDCMDFFGSYDVERPTGAFKRWKLFDLEFGRHNSGHQAELKLRDRLAGHE
                     ATQQAFYRILDGFEREGRANRLVLLHGPNGSAKSTFAACLMRGLEAYSDTDAGALYRF
                     SWIFPRAHEGAGIGFATSSDGLGKGDTFAHLPDDRIIAKLQSSVREHPLLLLPRDVRQ
                     RLISNIYESAGLSEAAPDWVWTGQLGRKNQQIFQALLTAYRGDLQQVLAHIQVERFYI
                     SRRYRVGAVTIGPQMSVDASERQITADRSIDSLPASLSALTLFEPYGELVDASGGIVE
                     FSDLLKRPLEAWKYLLLAIETGEVSLQMSNLPINAVMVASSNELHLSAFRQHPEYNSF
                     RGRIVRIRMSYLLDVHSEREIYDGQIVPQIPKHVAPHTTYVAALWAVLTRLRRPDASL
                     FSDSELGTIAASLSPLEKADLLADGVVPARLESDQAKILAAGIAEVEQQGANSSDYEG
                     INGASPREIRMLLLDSAANFDEPCVSPATFLNRLAEFCARDDYDFLKEQSDGLYRDHR
                     AFVDVVKERWLDIIESELRAASGLIDEQSHLGLFDRYVTQVSNWVKKERVFNPLTGKD
                     EEPDLDLMKSVEENLGADDPQTFRNELLSSIAAWAIDHPEQEVDYESLFPRYIEQVRQ
                     AYFGERRDQIGEIGRAIVSVLEDDDAIEAVLRESAQQALQVLVGVYGYERSSAKVALG
                     ALIDARYHH"
     gene            complement(10842..11378)
                     /locus_tag="VFG22_17640"
     CDS             complement(10842..11378)
                     /locus_tag="VFG22_17640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418123.1"
                     /translation="MQMRIQTSLTALSLLITLSACERGAPAPKATGHDHSRHNHDHPL
                     SDLPAEAPKPDEPAGPSVETGSFLLAVTQAGPGYSIGKTGEVEIALEGRGDWHVNQEY
                     PIRVDLKAGSGVALQKTELLKEDAKEFGEEKVRFLAGVQPSTAGEHELTCDVSFAMCT
                     EENCILEKRTVAMQLRVE"
     gene            complement(11429..11974)
                     /locus_tag="VFG22_17645"
     CDS             complement(11429..11974)
                     /locus_tag="VFG22_17645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418124.1"
                     /translation="MRALIAAIACWGIGCAGATSNVPASDFRPSDASLFDQAVDRVAT
                     PVTVDGDLSGVFEHRVVRSDFIAVVRVASLSSDWVKRQSAYRLTVKIKERLKGSSPKE
                     LVLIVHDDEPGYESVKSNEDRLLRDSFVAFLKWENQAGASERIVHWHLSPNSDAVREK
                     IDYLLGRPASDPNATVEPDRP"
     gene            complement(11985..13010)
                     /locus_tag="VFG22_17650"
     CDS             complement(11985..13010)
                     /locus_tag="VFG22_17650"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET6418125.1"
                     /translation="MSRPPRRPRAPLPPGCDPASIILTFPVAPEHAGQRLDRFIQHRI
                     PRLSRTRAQEVVRACAYRADGSRRRASERVRTGELVVLVRPPFEEPNVPLFFDVLYED
                     DDVLALDKPAGLPVHPSATYHKNTLTYLLRQRFGENAPQIAHRLDRETSGLLLCGRTR
                     AAERDLKNSFENRRVRKRYLAIVRGVMPEDEGKIELPIDRAREGLHILMEVTPEGEGY
                     PSLTRYRVVARKEDATLVALTPQSGRQHQLRVHLSALGFPIIGDKLYGPEGSEPFLDY
                     IETGMTDALRRRLGHDRHALHAYELEFMHPAQGEPMTLTAPFAPDLVRLWGESLDPSA
                     FASASAC"
     gene            complement(12994..13185)
                     /gene="rpmB"
                     /locus_tag="VFG22_17655"
     CDS             complement(12994..13185)
                     /gene="rpmB"
                     /locus_tag="VFG22_17655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014398134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HET6418126.1"
                     /translation="MAQTCDFCGKNRHKAHKVSHSNIKTRKWQQPNLQSIRALVDGEM
                     RRVSACTRCIRSGKVVKAA"
     gene            13291..15327
                     /locus_tag="VFG22_17660"
     CDS             13291..15327
                     /locus_tag="VFG22_17660"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HET6418127.1"
                     /translation="MRAAGAITGLLVLATFVIPGTYGRKTEIASGPGLAAQPSVFPSS
                     ELDVGFATLAGRIYDETGRPLAGATVSLAGSGFWPARSVRSGTDGRFFWPEIPTGIYE
                     LRASKGSLVAPEIEGLILDAGARRAFAIQLAKGWTLLGTVIDVFDGRAIPNAEVTVRS
                     AVLGLHTRQGRSDANGGFELPGIVGDELSLYVEADGYVAAGPLRHAAAEPSLTVRLER
                     ASAIEGHVVDERGRPIEGALVRAFGEDELRDTPPSGADSLKVTAGPVPPISAAASEAL
                     AFIRQVESNRDGSFRLGNLRPGPYTLAASHDEFSSAESEPIRLKPGATRSDVTLRMRA
                     GAELAGRVVDERGDGLEGIPVELRVAGERLPQMSVTASDGSFSFRGLSGELSVTALPY
                     DLPPVRASISIEERPHVTVELALPSTLYTLRGRVVDERGFGVGGALLSVSSSRAETPV
                     RRNAKSDADGMFSVPALPAPPFELSAEHPAYGPTRLADIERIDDVRVVMSTGVTLLGE
                     VRDSWTRDGLGGVLVTLDGPIDPSTRSRSDGTFVFRQLPTGTYEVSFSHADYESQRRR
                     VVLEPPQYVDRPQELDTVRLQPGGSIEGEVLDARSEPVPNAEVAWGDPPRWDLAARSD
                     ARGRFVLRGVPAGAIWITARHPSAGENSSNRLITVRPVETSPGAFVRLPGSVLE"
     gene            15361..16272
                     /locus_tag="VFG22_17665"
     CDS             15361..16272
                     /locus_tag="VFG22_17665"
                     /inference="COORDINATES: protein motif:HMM:NF016658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HET6418128.1"
                     /translation="MTRSWVRNIRDAGDILRRARSPVELARALVPNQWLARKVLNDGT
                     GAASEASASEVAESLRKLRNQLKQEALDERGQVDYAKLRGSATYAELARTSGLLHSVD
                     PAEMSDDDTRIAFWINLYNVLAIHGVLALGIRESVMEIPSFFGVVAYRVGDYVLTLDE
                     IENGVLRRNSPHPATKACLFDDDDPRLALCPSRIDPRIHAALVCASTSCPPVAFYEAS
                     KLEGQLDLAALNYVASDVEVDDRSRVVRMPITFRYYESDFGRRAGVLVFLLAHARGAQ
                     HEALQAAFDAGYALDYHRYDWSLNAMA"
BASE COUNT         3109 a         5128 c         5105 g         2969 t
ORIGIN      
        1 aacggatgcc ccaatctcgg ctccccaaaa ccgaacggca cgaatggctc gaggcgcggt
       61 gcaacccaga gcgccccagc gaatgcaaga gcaagcgcga ccgctacggt tcgacgtgcc
      121 gagcgaatcc attcgagctg cgcgaagccc gcgccgatga agggcgccgt caagagcagt
      181 tggtgtgcga cgaaccggat cgatcgaaac gccatgaggc tcaggagcac acaaaagaca
      241 ccataggcca tgccgtagcg actcccccga acgacgggac gcatcgcaac gagcagcaag
      301 atggcggccg ccatcggccc gaggagcagg tacaggggat gccccggcga ccagggggcc
      361 cactcggtga ccagctcccg gtactcggga cggaacacgt gggaaatggc atcggtgacg
      421 atatcgatcc cgaacggcgt gcagaaggtc gccaccgttt gcacggcgag caggctcagc
      481 atggaggtga acgccccagt ccgtatcgag aacagcaaga agagcgccgt gatcagcagg
      541 ccgagtagat gcgagccgtg catgttcacc caggcgagct gcatcgcgcc aagtgcaaag
      601 accagcccga gcttcgcgcg cgacgagagc cgctccgagt acaatgcgtg gatccccaga
      661 aggagcacgg cgggaaacac cagcccgatg atctgcggtc ggacggtgaa gcgaattcgg
      721 gccagcggcg cgaagaggat cgcgaccgtt gccgcaagcg gtgctgcaag cgctgcgcct
      781 ttggccaggc gggcggcgag caggacgagg acgtagccga gcaccgcgag gaggatgcat
      841 ttgatgccga tcagaacgtt tggaccgaag cggtcgtaga gaagccagct gagcagatca
      901 taaccccact cgtagttgct ccagggggct gggctcggct tccaaaagga gaacggatcg
      961 agctccggta cccggccgag ctgcgcgatc tgccggccct gggcgaggtg tccataggcg
     1021 tcgaggctgt agagaggaaa cgcccccatg agagagagcg cgagcgcaga aacccacgca
     1081 ctcccagaga cgcccttccc caacaccggc ttagtgttcg tcagaccctt gctggcggtc
     1141 aaacttcact caggcgtgcg gaagatgatc gtccctacgt cggggccgag gagcccgaga
     1201 ggaaagacgc catatacctc tagcgctccc tgaaggcgga ccttttcggt gtagccggcg
     1261 ggggagctag cgagtccttt cgcccgagcg ccgggatctc tcaccagagc atcgacttcc
     1321 gggggcgtgt cccagtagaa taggcgattg cccttgtaaa gatagaccca gaccggagcg
     1381 ccttcggcct gctcgacccg gaactgccgg ctcagacgct gcgcaagacg cgataaggga
     1441 atgcccgcga gaacgactcc aagcacctgg ccgtcacgga ccgagggggc cgcgaacaag
     1501 atcgaccagg acgaaggcgc actcgggtcc tctgcgtaga actctccgag cccagtggcc
     1561 gccgcgcccc caagggcctt tcgaacgacc tcgaagcgct ctttgaagtc ctgtcctttc
     1621 atgcggtcgg cctcgcggtc gcgcgcgatc acccgcccgt cgataccgat cgcggcgaga
     1681 aatgacatcg gggaggcaac gaattcatca atgcctttct tgggttgctg aagaattctc
     1741 aacgccgcac ggacttgcga ctcccgcgcg accggatcgc tgagtgcgaa acccggaccg
     1801 agcttctctg cggcctcgag gacgccagcc tgatgcttct gcaagtcctc ctgaatcagc
     1861 gcctggaccc tcgggtagtc gacctcgcgc agctgctccc gggtgcgggc aagcgcggcg
     1921 tcatcttcgc accctgcgag cagggctgcg gcgagaaaga ggatccccca tggcatgggc
     1981 tcagtgtatc agggtcagcc ccagtgtcag ggtcagcctc agtgctagtg cccgcgccag
     2041 tgcccttgcc agccgtccca tcgacaagct ctccgaccgg cgttacccgt ggatctggag
     2101 ggtatcacca tgtcccagcc tattttgcaa acgagctccc cgacgtcgcg tcccgagtcg
     2161 atcacggccg tcgagcttca catgatgccc cgcgacgagg tgctggccct ttatcaccaa
     2221 ctcgaggcgc cagcgttcga agagatgcac ggcgagttcg cggcctccct cctcgatcaa
     2281 ggccatgcac tcggatacct gctgagcgcg ttcgccgtga acgcgaaggg ccgttggctc
     2341 tgcaaggctt tcgagcccag tggacccaac gaagggcacg gatacaacag cttcatgacc
     2401 ccgcgcggcg tgaggcgatc ggcgcgaatg cgaacgcaca tcgggccctc caaattacct
     2461 ggggacacga aggacgcgtt tcatctggag tacgcggact tcaacgactt ccaacgaggc
     2521 ggccccggag gcgcgctgct ccacacgatg ttcgacgagg tccgcaaggt tcgagcgggc
     2581 ctctatctcg gaatcggccg cgtgggcttc accaagaagg cgcggagcga gccccacccc
     2641 ttcatcttgg agggacccgt tgcacgattc atgagcgcga ggctctgagc gcgaccgttc
     2701 tcagccgccc gagcagaagt gctcgtagtc gatatactcg gtgatcgtcg gatggtctcc
     2761 gcagaccggg cagtccttgt tccggcgaag cttgaaggtc cggaactgca tctgaagcga
     2821 atcgtattgc aagagacgac cgaccaaggg gtcgccccgc tcgagcacga tcttcacggc
     2881 ctcagtcgcc tgaagggtcc cgatcgctcc gggaagcacg ccaaggacgc ccgcttcggc
     2941 acacgacggc gccaaatgcg gcggaggcgg ctccgggtac aagcaacggt agcatggtcc
     3001 gtgatgcggc cagaaggtcg taacctgtcc gtcgaaccgg aagatcgagc cgtgcacgac
     3061 cgggatgtcc ttccaaacac tcgcgtcgtt caccagataa cgggtcgcga agttgtcgag
     3121 cccgtcgacg atgacgtccc agtcctgatc gaagatccga tcgacgttct ggcgggtgag
     3181 gcgctcgttg aacgggatga ccttcacgtc ggggttgaga ttctcgatcg tgcgcttccc
     3241 gctcatcacc ttggactcgc ccacgcggtc gagtccgtgc aaaacctgcc gctggagatt
     3301 cgactcgtcc accgtgtcgc catcgacgag accgatcgta ccgatacccg cggcagcgag
     3361 gtagagcgca gccggagaac ctaggccgcc agcaccgagc aagagaacgc gcccctgcag
     3421 gagcttggct tggccctgct ctccgacctc cggcaacaag aggtgtcgcg agtaacggtt
     3481 gagttgtgca tcggtgaacg caaacggctg cgtcgtcggg aatccctgat ctttccattg
     3541 attgaatcca ggattggccg actcgacgtt gttgtagccg aggtcctgaa gaaccttggc
     3601 agcgaacgcc gaccggatcc ccgccgcgca gatcgtgacg atggtcgcgt ccttgtccgg
     3661 cagccgcgaa cccgattgca tttcgagaaa gcctcgcggg atgtgaagcg cgccaggaac
     3721 gtgcccttgc cgccactcgt ctttctcccg aacatcgacc agaatcagct cttcgcgggc
     3781 atctaggcgg ctcttcaagt cctcgagaga gacctgccga atggcgccct tcacctcttg
     3841 gagcatgtca gcgtatgtct tggccatgct cgccactatg taacttcggg tagtttcgtc
     3901 aaggctaacg ggggcttaga gcatatctaa tggttttcac atcggttttg tcgggtatgt
     3961 cgttccgagc ggcccgagct aggctgcgga gctgaggatt ccctcttccc gggcgtagtc
     4021 ctccagcacg gacttgaggc gctttcgacc cctgtgaagt cgggacatca cggtgcctac
     4081 cggacactcg agccggaacg cggcgtcctg atacgaaagc tcagcgatgt cgaccagctc
     4141 caccgcctct cggaattcag gacgcaactg ggtcagggcg gccaggactt catcgctgag
     4201 gacttcgctg ctggagcgat ccgagctgca agcgagacgg cagagctctt cgagcgcctc
     4261 cttctcccgc tttcgtttgc gatagtggtt gatgtacgtg ttgaacagga ttcgttgcgt
     4321 ccaggcgcgc gcgttggtgc cggggcggaa gcttatgcgc gcttgccagg cgcgggccaa
     4381 ggtttcctga accaggtcgt cagcgagggc agtgcctcgg gccagtcgaa gcgcgctcct
     4441 ggtcagctcg ggcacgcagg gcagtatttc ttcgttgtac tgttctttgg tctgcatcga
     4501 gatggtctcc acctcgacct cattgcaacg gcgatgccaa tcgaaaaacc caagcatttc
     4561 gggcgcggca gcagccatgc agcgtcagct tgtcgtccgg gtgacacggc gtcgtgtcaa
     4621 gctgtcgcct gcctagacgc tcagtagccg agctgctccg ccaagacagc gggcacggca
     4681 tcagccaatt cagaggcgat caagcccctg tcgccggtcg aggcggcgat accggcaagg
     4741 gcgtggagga gcgcccccgc gattgcagcg tcgagcggcg cgagactcat cgccagcgcc
     4801 cccaccactc cgctcagcac gtcacccgtt cctgctactc caagtgccgg cgtgccctct
     4861 ccgcaaacgc gaagcgcgcc cgaccccgcc acgatgctcc gggcgccctt caagatgacg
     4921 gtttggcccg actcctcggc gagggctgcc gcggctgcgt agcggtctgc ttgaacgtcc
     4981 gcgctcgagc ggccgagcag gcgagcggcc tctccaggat gtggggtgag aattcgaggc
     5041 gcggccgcgc ctttgagcgc agcaaggcct tcgctgccga ggttggtgag accgtcggca
     5101 tcgatgatgc tcggaacggg ggcgtgaagc gcaacgcaat gcgcccagct acggcccagc
     5161 tcgtcgagac ccattccggg tccgagcacg actgcccgca ttccctcgca gagtgcttcg
     5221 accgcgcttt gatcgacgtg ctcaggaagc gagctcgtca tcagctcgat caccttctct
     5281 tcgagcgccg ctcgtgaatc ggcgcgggca ccgatcgtga caagacctgc tccggcgcga
     5341 aacgcggcgc gacccgcaag cagtgccgcg cctgtttttc ccggggcgcc cccgatcacc
     5401 agcaggtgac cgttggtgcc cttgtgtgca tcgggagcgc gtcgagcgat cagcgccggg
     5461 agatcatcgc cggtccacaa actcgctgcc ggtgcctgga tgacccgaat gccgatgtct
     5521 gcgactcgga cttcgccagc gtgttgcaca ccggggtatt gatggaggcc acgcttctgc
     5581 gcggcgaacg tgaccgtcag cgccgcctgc gccgcgacgc cgagaatcgc cccggtgtct
     5641 gcatcgagac cacttggaag gtccaacgcg accagcggtg ccgatgcgcg attcatcgca
     5701 aggatcgcgt cggcgtagcc gccggtgacc ggtcgatcga gcccggtgcc gaaaagtcca
     5761 tcgacgatca acgtcgcatc cgccaaggtg ccttcgatcg actcggcatt ctcgggtgcg
     5821 acttctaggc tctcgacccc gagcttcccc agcaacgccc agttcgcacg tgcgtcgccc
     5881 cgcaacctcg atgcatcccc cagcaagacg gcgagcggct catgtccgag cagtgagaga
     5941 cgccgggcca cgacccaggc atcgcctccg ttttgaccgg gtccgccgat cagtacgaca
     6001 cgtcccaggc gatctccgaa cgaccggcag atgagatcgg tggcgccacg tccagcgttc
     6061 tccatcagca ctaggctggc gactcctagc tcgtccatcg ctccgcgatc cacatcgcgg
     6121 gactcgtcgc gtgtaaatag ctgctgcacg attcgattat gcctttaatc cggcttcggc
     6181 acaccctatg ctcagcccat gaatgcgaaa cacccttcgg agtcgttcac ggagatgacg
     6241 gagctcgttt tgccgcagca tacgaatgca atcggtaccg cgttcggcgg ggtcatcatg
     6301 agctggatcg acatctgcag ctcgatcgcg gccaagcggc attgcggaaa cgtctgcgtg
     6361 accgcccgag tcgacgcgct cgaattcagg gcgccgatca aggtcgggga cgtcgtgcgc
     6421 ttgaccgcgc gcctcaatgg ggcctttacc agctcgatgg agatcggcgt ccgagtagag
     6481 cgcgagcatg gacggacgag tcaaagaacg ctctgcgccg atgcgcgagc gaccttcgtc
     6541 aacctcggag aggacgggca gccctgccct gtccccccgc tcctggctgc aagcgaagag
     6601 gacaggcagc tcgcggcgca ggcggctgcg cggcgagatc aacgacgtct tgggcgcaac
     6661 gtgtgaatcg gagccattct ctcatgccca tccttccccg agcacaactc ttcgagttca
     6721 acgaccgcga ctgggttccc gagagcttgc gcgacaccat cgtcgaaacg ctcagccgtt
     6781 cgctggactg gggcggtttt ctggcaccgc tcgcaccggt gatcgacgag ttcctctcgg
     6841 cggcaggcac gactgaggtc ctcgatctct gcgctggggc gggcggccct gcgctgatcc
     6901 tgagccagga agcggaacgg ctgggcctcg atgctccacg atacctgatg accgatctct
     6961 acccgcgtgt gcccctgtgg accgaagcta cacgtcgacg accacgaagc atcgacttca
     7021 tcgaggaccc cgtcgatgcg acgcgaatcc ccgcatcgat ttcccgaggt cgcgcgcgcg
     7081 tgatggtcaa cgcgtttcat cactttcaac ccgaaatggc gggtgccatc ctccgtgacg
     7141 cggttgaaca ggaagcgtcc atcctgattt cggaatcgtt cgagcgtgat ccgcgaggtg
     7201 cgtttccgat gattccaatc ggcatccccg cccttctcgc cacgcccttg ctctcgcccc
     7261 gcaatcgact cgccaaagct gcctggacct ggctcacgcc catcgccctc gccgcgggcg
     7321 cgtgggatgc gatcatcagc accctccgta tctactccga atcagatctc cgggatctcg
     7381 tggccccatt tggacacggc tatcgatgga agtacgggac gtacgactac ccgttcggtg
     7441 gacgaggctg ctattttcag ggcgtccccg cggcggccca acctgtctcg cccggaaaac
     7501 cggcagatgg cctgcggacg tttccgagga agcccaatgg ccctagcgcc ctgacagaca
     7561 ggatgacgat ggacgacgaa aagacttacc aagataccga cggccaacgg cctggtgacg
     7621 gcgacgcaac gattgctcgg gaggcgaacg cgaggcgcgc tgagctggcc gctgagctga
     7681 agcaggaggt cgatgcactc gaggagccct ccggacccca gacggacgcg cggaccgcag
     7741 aagccatgag gaagaaggcg ttgcgccggt atctagagga gcagagcctc aggccctatc
     7801 aagccgagct gatcaagatg cagcggtacc tcgaagagag cggcacgcgc atgatcatct
     7861 tgttcgaagg gcgcgatgct gccggcaagg gcgggaccat ccgtcgagtg acgcgataca
     7921 tgaacgagaa gcactaccgc gtcgtcgctc tcgggaaacc taccgagcgg gagcggacgg
     7981 agtggttctt ccagaagtac gtccaacact ttccccgcgc tggcgaagtc gttctgttcg
     8041 accgaagttg gtacaaccgc gccatggtcg agccggtctt cggattctgc accgatagag
     8101 aatacaaaaa cttcatgcgc ggggtcactg gcttcgaaaa agacctcgtc cgccaaggca
     8161 ccgtcttgat caagctctac ttcagcgtga cgaaggacga gcagaatcgt cgtttcgaac
     8221 gtcgcaaagc ggacccgctt cgccaatgga aactgagtga ggtcgatgtg caggcccagg
     8281 agcgctggga cgatttcacc aacgtcaaat acgaaatgct caagcgtacg cacacgacgc
     8341 atgccccttg gaaaatcatt cgctcgaacg acaagcatgc ggcccggttg aacgccctca
     8401 agctaatcct gaactcagtc ccgtacgagc ggctcgatga gagcctcgat ttcgtgccga
     8461 gctccgaaat cgtgatctct ggggcgcggg agctcgaaaa gatggaagcg cagcgccttc
     8521 gaagcggaaa atttctcgtc tagccggggg tcgctgccga aagaccgggc gaggcttgcg
     8581 gcccgccgcg tcagtggtgg tatcgcgcgt cgatcagagc tccgagtgcg acctttgcgc
     8641 tcgagcgctc gtatccgtag acgccgacca agacctgcag cgcctgttgc gcggactctc
     8701 ggagaacggc ctcgatggca tcgtcgtctt cgagcactga aacgatggca cgcccgatct
     8761 cgccgatctg atcgcggcgc tcgccgaaat aggcctggcg cacttgctcg atgtagcgtg
     8821 ggaagagcga ctcgtagtcc acctcctgct ccggatgatc gatggcccag gcggcgatcg
     8881 agctgagcag ctcgttccga aacgtctgag gatcgtctgc gccgagattc tcttcgacgc
     8941 tcttcatcag atccaagtct ggttcttcgt cttttccagt gagcggattg aacacacgct
     9001 ctttcttcac ccagttcgaa acctgcgtca cataacgatc gaacaaaccg agatggctct
     9061 gttcgtcgat cagaccgctg gcggcacgga gctcgctctc tatgatatcg agccagcgct
     9121 ctttcactac gtccacgaat gctcgatggt cgcgatagag cccgtccgac tgctccttga
     9181 gaaaatcgta gtcgtcgcgc gcgcaaaatt cggcaagccg attcaagaac gtcgcaggcg
     9241 agacgcatgg ctcgtcgaag ttggccgcgg aatcgagaag aagcattcga atttcgcgag
     9301 gcgaagcgcc attgattccc tcgtagtcag aggagttggc gccttgttgt tcgacttctg
     9361 cgataccggc ggcgaggatc ttggcctggt ccgactcgag tcgcgcaggg acgaccccat
     9421 ccgctagaag atcagccttt tcgagcggcg agagagatgc cgcgatagtg ccgagctcgc
     9481 tgtcgctgaa tagagaagcg tcgggccgac gtaggcgcgt gagcaccgcc cagagagccg
     9541 cgacgtaggt ggtgtgcggc gcaacatgct ttgggatttg tggcacgatc tgaccatcgt
     9601 agatttcccg ttcgctgtgc acgtcgagca gatacgacat gcgaatgcgt acgatgcgtc
     9661 cgcgaaaaga gttgtactcc ggatgctgcc ggaaggcgct caggtgaagc tcgttggagc
     9721 ttgcgaccat caccgcgttg atgggcaggt tagacatctg caacgaaacc tcgcccgttt
     9781 cgatcgcgag caacaagtac ttccaagcct ctaggggtcg tttcagcaag tcgctgaact
     9841 cgacgatgcc cccggaggca tcgaccagct cgccgtatgg ttcgaacaag gtcagcgcac
     9901 tcagtgatgc aggcaaggaa tcgatggagc ggtctgcggt gatctgccgt tccgaagcat
     9961 cgacggacat ctgcgggccg atggtcacgg cacctacgcg atagcgccgt gagatgtaga
    10021 aacgctcgac ctgtatgtgc gcgaggacct gttggagatc tccacggtag gccgtgagca
    10081 gcgcttggaa gatctgttgg ttcttgcggc cgagctgacc agtccagacc cagtcgggcg
    10141 cggcctcgga tagacctgcc gattcgtaga tgtttgagat caagcgctgc cggacgtcgc
    10201 gcggcagcaa gagtagaggg tgttcccgca ccgagctttg gagcttggcg atgatccggt
    10261 cgtcaggcag gtgcgcaaag gtgtcgccct tacccaagcc atcggaggag gttgcgaagc
    10321 cgatgccggc gccctcatgt gcacgtggga agatccatga gaatcgatag agcgcccccg
    10381 catctgtgtc cgagtacgcc tcgagccctc gcatcaaaca tgccgcaaag gtcgacttcg
    10441 cgcttccgtt cggtccgtga agaagcacca ggcgattcgc tcggccttct cgctcgaatc
    10501 catcgagaat ccgatagaac gcctgctgcg tcgcctcgtg acccgccaga cgatcgcgaa
    10561 gcttcagctc ggcctgatga ccgctgttgt ggcgcccgaa ctcgagatcg aaaagcttcc
    10621 agcgcttgaa cgcgcccgtt ggccgctcca catcgtacga gccaaagaaa tccatgcagt
    10681 ctttgagata gcggcctgca tcccgcgtgt agcgccgcgg attgtcttga acgagctcga
    10741 ggtactcccc gtaggagagc actcgtttct gggcctcgaa ccgcgctttg acttccccac
    10801 gaatcgtctc caacgcggtc ttgagtcccc cctcgctcat ctcattccac cctgagttgc
    10861 attgcgaccg ttcgcttctc gaggatacag ttttcttcgg tgcacatggc aaaggaaacg
    10921 tcgcaagtca gctcgtgctc gccggctgtg gaaggttgga ccccagctag aaaccggacc
    10981 ttttcctcgc cgaactcctt tgcgtcctcc ttgagcaact ccgtcttctg gagcgcgacc
    11041 ccggacccag ccttcaggtc tacgcggatt gggtactcct gattgacgtg ccagtcgcca
    11101 cgtccctcga gagcgatctc cacctcccca gtcttgccaa tggaatagcc gggcccggcc
    11161 tgggtcacag caaggagaaa ggagccggtt tcgacgctgg gacctgcggg ctcgtcaggc
    11221 ttcggggcct ctgcgggcag atcgctgagc ggatgatcgt gattgtgccg ggagtggtcg
    11281 tgcccggtgg ctttcggagc cggcgctcct cgttcgcacg cgctcagcgt gatgagcaag
    11341 gacaaggcgg tcaatgaggt ttggatacgc atctgcatca ctcctggctt agtgtacgac
    11401 gtaggcgctg gttacgtcga agtccattct agggtcgatc tggctcgacc gtggcgttgg
    11461 ggtcgctcgc cggccggcca agtaggtagt cgatcttttc gcgcactgcg tccgaatttg
    11521 gcgagaggtg ccaatgcacg atcctctccg aggccccagc ctggttctcc cacttgagga
    11581 atgcgacgaa cgaatcgcga agcagccgat cctcgttgga ctttacgctc tcgtaacccg
    11641 gttcgtcgtc gtggacgatc aggacgagct ccttcggcga gcttcccttg agtcgttcct
    11701 tgatcttgac cgtcaagcga taagccgact ggcgcttgac ccaatctgag ctgagggacg
    11761 ccactcgaac gactgcgatg aagtcggagc ggaccacacg atgctcgaac acaccgctaa
    11821 ggtctccgtc gacggtgacg ggtgtggcaa cgcgatctac ggcctgatcg aaaagagagg
    11881 cgtccgaggg tcgaaagtcg ctcgccggca cgttggatgt ggcccccgca catccgattc
    11941 cccagcaagc gatggctgca atcagcgcac gcacgggttg gagcttagca cgccgaagcg
    12001 gaggcgaatg ccgacgggtc cagagactcg ccccagaggc ggaccaaatc gggagcaaac
    12061 ggcgccgtca gtgtcattgg ttcgccttgc gcagggtgca tgaactcgag ctcgtaagcg
    12121 tggagagcgt gccgatcgtg gcccagccgg cgcctgagag cgtcggtcat cccggtttcg
    12181 atgtagtcca ggaagggttc ggagccctcc ggaccgtaaa gcttgtcgcc aatgattggg
    12241 aagccgagtg cgctcaaatg gacgcgaagc tggtgttgtc ggccactctg aggggtaagc
    12301 gcgaccagcg tggcgtcttc cttgcgcgca accactcgat agcgcgtgag cgagggatac
    12361 ccctctcctt caggagtgac ctccatcaag atgtgaaggc cttcccgcgc acggtcgatc
    12421 ggaagctcga ttttaccctc atcctcgggc atgacgccac gcacgatcgc cagatagcgt
    12481 ttgcgaacgc gacggttttc aaagctgttc ttcagatcgc gctccgcagc gcgcgtgcgt
    12541 ccgcaaagca gaagcccact ggtctcgcga tcgaggcgat gcgcaatctg tggcgcattc
    12601 tctccgaatc gctgtctgag caaataggtg agcgtgttct tgtggtaggt ggccgacgga
    12661 tgaaccggta ggcccgcagg tttgtcgagc gctagaacgt catcgtcctc gtaaagcacg
    12721 tcgaagaaga gcggaacgtt gggctcttcg aatgggggtc gaacgagcac cacgagctcg
    12781 ccggtccgaa cgcgctcgct cgcgcggcgg cggctgccat cggctcgata tgcacaggcc
    12841 cggacgacct cttgagcgcg cgtcctggag agcctcggga ttcggtgctg gatgaatcga
    12901 tcgaggcgct gcccggcgtg ctcgggtgca acgggaaagg tgaggatgat cgaagccgga
    12961 tcacaacccg gtggaagcgg ggcccgtgga cgtctaggcg gccttgacaa ccttacccga
    13021 acgaatgcag cgcgtgcagg ccgaaacgcg gcgcatctcg ccgtcgacca gcgcccgaat
    13081 cgactgcaga ttgggctgct gccacttccg ggtcttgatg ttcgagtggc tcaccttgtg
    13141 agctttgtgg cggttcttac cgcagaaatc acatgtctgt gccatggtcg gtttccgaat
    13201 cttcagggag ccgcgatgta gcaccggtgc cgaggtaccg caacaatgcg acctcgcccc
    13261 cagccccaat cgaagataaa accaagcgag atgcgagcgg cgggcgcaat cacggggctt
    13321 ttggtcctcg cgactttcgt aattccgggc acatatggcc gaaagacgga gatcgcctca
    13381 gggcctgggc tcgcggccca gccatctgtg tttccctctt cggagctcga cgtgggcttc
    13441 gccaccttgg cggggcgaat ctacgacgag accggccgcc cgctcgctgg ggcaaccgtc
    13501 tccttggcag gcagcgggtt ctggccggcg cgctcggtac gaagcggtac cgacgggcgc
    13561 ttcttctggc ctgagatccc gaccgggatc tacgagcttc gcgcgtcgaa agggtcgctg
    13621 gtcgcccccg agatcgaagg gctcattctc gacgccggcg cccgccgcgc attcgcgatt
    13681 cagctggcga aaggatggac gctactgggc acggtcatcg acgtcttcga tggacgagcg
    13741 atcccgaacg ccgaagtgac cgtcaggtcc gcggtcctcg gattgcacac gcgccaaggt
    13801 cgaagcgacg ccaatggcgg attcgagttg ccgggaatcg tcggcgatga gctgagcctc
    13861 tacgtggagg cggacggata cgtggcggct ggacctctgc gccacgcggc cgcggagccg
    13921 tctctcacag tgcgtctcga gcgggcatcg gcgatcgaag gccacgtcgt cgacgagcgc
    13981 ggccgcccca tcgaaggcgc gctagttcgg gctttcggag aagatgagct gcgcgacact
    14041 ccgccttcgg gtgccgattc gctcaaggtc actgccgggc ccgttccgcc gatctctgca
    14101 gccgcgagcg aagctctggc gttcattcgc caagtcgaat cgaatcggga tggcagcttt
    14161 cgcctcggca atcttcggcc gggcccgtac acgcttgctg ccagtcacga cgagttctcc
    14221 tctgcggagt ccgaacccat tcgtctgaaa cccggcgcga cacgctcgga cgtcacgctg
    14281 cggatgcgtg cgggagcgga gctcgccggc cgcgtggtcg acgagcgagg cgatggactc
    14341 gaaggaatcc cagtcgagct tcgcgtggcc ggcgagcgcc taccgcaaat gtcggttaca
    14401 gcatccgatg gctccttttc ctttcgcggt ctgagcggcg agttgagcgt cacggcattg
    14461 ccctatgatt tgccgccggt tcgcgcctcg atctcaatcg aagaacgacc gcacgtgact
    14521 gtcgagctcg cacttccgag cacgctctac acgttgcgcg gacgggtcgt cgacgagcgc
    14581 ggatttggtg tcggcggcgc actgctcagc gtttcgtcga gccgagcgga aaccccggtc
    14641 cggcggaacg cgaaatccga cgcagatggc atgttctccg tgcctgccct tccggctccc
    14701 cccttcgagc tgagtgccga gcacccggcc tacggtccga cgcgactggc cgatatcgaa
    14761 cgcatcgacg acgttcgcgt cgtcatgtcc acgggcgtaa ccctcctcgg cgaggtccgc
    14821 gatagctgga cgagagacgg gctcggaggt gtcctggtca cgctcgatgg cccgatcgac
    14881 ccgagcaccc gcagccggag cgacgggacc ttcgtgtttc gccagctacc aacgggaact
    14941 tacgaggtat ccttctcgca cgcggactac gaatcacaac gtcgccgtgt ggttctggag
    15001 ccccctcagt acgtcgaccg tccacaggag ctcgacacgg tccggctcca accgggtgga
    15061 agcatcgaag gtgaagtgct ggacgcccga agcgagccgg tgccgaacgc cgaggtcgcc
    15121 tggggagatc ctccgcgatg ggatctagcg gctcggagcg acgcacgcgg gcgattcgtt
    15181 ctacgaggcg tccctgcagg ggcgatctgg atcacggcgc ggcacccgtc agcaggcgag
    15241 aattcgagca accggctcat cacggttcgc cccgtcgaga cgtcgccagg cgccttcgtg
    15301 cgcctgcccg gctccgtctt ggagtgacga aaccgggatt gcgccgtacc ctcctctgag
    15361 atgaccaggt cctgggttcg gaacatacgc gatgcgggcg acatcctccg gcgagcgcgc
    15421 agtccggtcg agctggcccg tgccttggtc ccaaatcaat ggctcgcgcg caaggttttg
    15481 aacgacggta ccggtgccgc ctcggaggca tcggcaagcg aggtagccga atcgctgcgg
    15541 aagctgcgta accagctcaa gcaagaggcg ctcgatgaac gcggccaagt cgactacgcc
    15601 aagcttcgag gctccgcgac ctacgccgag ctggcgagaa ccagcgggtt gcttcactcg
    15661 gttgatcccg cggagatgtc cgacgatgac acacggatcg cgttttggat caacctctac
    15721 aacgttctcg ccattcatgg cgttctggca ctcggcatcc gtgagtccgt catggagatt
    15781 ccgtcgttct tcggtgtagt ggcttatcga gtgggtgact atgtcctcac tctcgacgag
    15841 atcgagaacg gcgtgttgcg gcgaaactct ccgcaccctg cgacgaaggc gtgcctgttc
    15901 gacgatgacg atcccaggtt ggctctttgc ccttcgcgca tcgatcctcg catacacgcg
    15961 gccctggttt gcgcgtcgac cagctgtcct cccgtcgcct tctacgaggc ttcgaagctc
    16021 gaggggcagc tcgatcttgc tgcgctaaac tatgtggctt ccgacgtcga ggtcgacgac
    16081 aggagccgtg tggtgcgcat gccgatcacg tttcgttact atgagagtga cttcggaaga
    16141 cgtgcgggcg tgctggtgtt cttactcgcg cacgcacgag gtgcgcagca cgaggcatta
    16201 caagccgctt tcgacgcagg gtacgcgctc gactatcacc gctacgactg gtcgctgaac
    16261 gcaatggcct gagcccgacc ccaggtgcca gcgccagtgc cagtgcccgc g
//
LOCUS       DASRZF010000398         4870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_5109198, whole genome shotgun sequence.
ACCESSION   DASRZF010000398 DASRZF010000000
VERSION     DASRZF010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5109198"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="VFG22_17670"
     CDS             <1..731
                     /locus_tag="VFG22_17670"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HET6418129.1"
                     /translation="ALGREAALRGVVVVSGGAIGVDRAAHEGAIDGGGSTLVVLPTGL
                     DEPYPPANRDLFERAIEAGCLLTEAEDGAAPQPGRFLTRNRLIAALGRSTVVVQAPAR
                     SGALSTARIAQRLGRAVFAVPASPWDSRGSGNLQLLSKGARICARPGDVLSEAAPERG
                     RQEARPRVQKEKSHDFNTLTQAEQTILASLGGRARHAEDLCQRTGIPAFEVQRSILRL
                     LVRGLIAEKPGGRYQSCRRGRKPY"
     gene            741..3140
                     /gene="topA"
                     /locus_tag="VFG22_17675"
     CDS             741..3140
                     /gene="topA"
                     /locus_tag="VFG22_17675"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HET6418130.1"
                     /translation="MSKTLLIVESPAKAKTIKKYVGAGYEVLASKGHIKDLPKKGGVD
                     IDNGFQETYELLEEKGKPEVVKAIRAAAKKADKVLLATDPDREGEAIAYHLMEVAQTA
                     NKDAEIKRVLFEEITKRGVKKGLDAPRDLDSNLYEAQRTRRVLDRIGGYPLSSLLWKK
                     LAFGLSAGRVQTPALRIIVDRQHERDAFVPVDYWIVDARLKGEKPHSFMASLQSVDGK
                     KLEKVGSRVAVLNEKDSARYVSDLKAADYLVKEISKRKASRKAPAPYTTSKLQQDAAN
                     RLSMPPKRTMRIAQQLYEGMTLGRGKNSETVGLITYMRTDSVRLSPDAVDECRAYVAK
                     KYGKDSVPEKPNFFKTKKQNVQDAHEAIRPTRLDLPPEAVSKYLNDPQRRLYKLIWDR
                     FVASQMTPAVFDQTGVEIEASREGRVYGLRASGSVLKVPGWRAVYGATTEPLAGEEPS
                     PEDRTDDDDSLPELREGEALQIVIPPGVEGHHKTTEPPPLFTEATLVKKLEEEGIGRP
                     STYAEIISKVEQRAYVEKDGNKLVPTPLGTLVIEKLVADNFDLADIGFTRKLEEDLDA
                     VGDARAKRLDVLQPFHDRLQAQIAHAVEQKGKWWPDPVEIGENCPESGDPLVRRWGRN
                     GPFIGCTKYPECRYTLNIGPDGEIEAPKEPELTEYDCHECGAKMSKRWGRNGWFLGCS
                     RYPECKHTRAVPLGFKCPKCKTGDIVPIKPKGRGRPFYGCTNYRSEEPCDFRVWQKPI
                     GMKCPNCGSDFLVAMGGKKNPKLKCANETCDFERPYDPSELADDGESEEVSPAADSAT
                     G"
     gene            3149..4525
                     /gene="trmFO"
                     /locus_tag="VFG22_17680"
     CDS             3149..4525
                     /gene="trmFO"
                     /locus_tag="VFG22_17680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HET6418131.1"
                     /translation="MADVVVIGAGLAGTECAWQLAQRGGHVRLIEQKPSSRTPAQHSD
                     QMAELVCSNSFRGAALHNAVGLLKEEMRRCGSLVMEVGELHRVPAGGAFAVDRDQFSA
                     EITRRVETHPLIEVVHEVVDRIPEERPVVIATGPLTSGGLADDIAAAVGAEHLAYYDA
                     IAPIVSADSINFERAFRASRYDKGGDDAYVNCPLNQDEYEAFVQALLDAEKVTPRDFE
                     KVRYFEGCLPVEVMAARGPKTLSFGCMKPVGLTDPRTGRWPKAVVQLRQEDEAGTAFN
                     MVGFQTRMTWPEQKRVFRMIPGLENAEFERLGSVHRNTFVNSPVVLDDDLSLRSRPGV
                     YLAGQISGVEGYVESAACGMLLGIKLAHELRGRVFSFPPGTTMLGGMIGHLREPNDDF
                     QPSNVMFSMVPTVGEPRLGKRARRDAQSERALQDLGVWLSSVVESNDALDGSARTADR
                     RLRSVPSG"
     gene            4464..>4870
                     /locus_tag="VFG22_17685"
     CDS             4464..>4870
                     /locus_tag="VFG22_17685"
                     /inference="COORDINATES: protein motif:HMM:NF014898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HET6418132.1"
                     /translation="MPSTDPLEQQIDAFALYLADERRSSARTVETYIRDLRSFRDYVL
                     DQGFTPDARELDIVALRGFLSSLFRSNQASTMKKKVSAIRSFFKFLLKRQMVNQNPAA
                     GLRSPKIAKTLPRFLSVDQAFRVMEAPPKEDKRA"
BASE COUNT          989 a         1503 c         1565 g          813 t
ORIGIN      
        1 acgcgctcgg gcgagaggcg gcgctccgcg gggtcgtggt cgtttcgggt ggtgcgatcg
       61 gcgtggaccg ggcggcgcac gaaggcgcga tcgatggagg aggaagtacc ttggtcgtcc
      121 ttccgactgg gctcgacgag ccgtatccgc cggccaatcg ggacctcttc gaacgggcga
      181 tcgaggctgg ctgtttgctg acggaggcag aggacggcgc cgctccccag cccgggcgct
      241 tcttgacgag aaaccggctc atcgcagccc tcgggcgctc gaccgtcgtg gtccaggccc
      301 ccgctcggtc cggcgccttg tcgaccgcgc gcatcgccca gcgtctcggg cgggcggttt
      361 ttgcagtgcc cgcttcccct tgggattcga ggggatcggg caaccttcag ctgctctcga
      421 agggcgcgag gatttgcgcg cgtcccggcg atgtcctatc ggaagcggca ccagagcggg
      481 gcaggcaaga agcccggccc cgcgtccaaa aagaaaaatc ccacgatttc aatactttga
      541 cacaagccga gcaaacgatc ctcgcctcct tgggaggccg cgcgcgccat gcggaggacc
      601 tttgccaacg caccggaatt ccggcgtttg aggtacagcg gtctattctg cggctcctcg
      661 ttcgcggtct gatcgcagag aagcctgggg gtcggtacca aagttgccga agaggccgca
      721 agccttacta aaggagatcg atgtccaaaa ccctcctcat cgtggagtcc cctgccaagg
      781 cgaagaccat caagaagtac gtcggtgcgg gctacgaggt tcttgcgtcc aaaggccaca
      841 tcaaggacct ccccaagaag ggaggcgtcg acatcgacaa tgggttccag gagacctacg
      901 agctcctcga ggaaaaggga aagcccgagg tcgtcaaagc gattcgggcc gcagccaaga
      961 aagccgacaa ggtcctgctc gccaccgacc cggaccgcga aggcgaagcg atcgcgtacc
     1021 acctgatgga agtggcccag acggccaaca aggacgcgga gatcaaacgc gtcctcttcg
     1081 aggaaatcac caagcgcggc gtgaaaaagg gtctcgacgc gcctcgcgac ctcgactcga
     1141 acctctacga agcgcagcgc acacgtcgcg tgctcgatag gatcggcggg tatccgcttt
     1201 cgagcctctt gtggaagaaa ctagccttcg ggctctcggc gggtcgcgtc cagaccccgg
     1261 cccttcgcat catcgtcgac cgccaacacg agcgcgacgc gttcgttccc gtggattact
     1321 ggatcgtcga tgctcggcta aagggagaga aaccgcattc gttcatggca agccttcaga
     1381 gcgtcgacgg taagaagctc gaaaaggtcg gctcgcgcgt cgcggttctc aacgagaagg
     1441 actcggcgcg ctatgtctcg gacctcaaag cggccgacta cctcgtcaaa gagatcagca
     1501 agcgaaaggc gtcgcgtaaa gcacccgctc cgtacacgac ctcgaagctt cagcaggacg
     1561 cagcgaaccg attgagcatg ccgcccaagc gcaccatgcg gatcgcgcag cagctctatg
     1621 aaggcatgac cctcgggcgc ggaaagaact cggagacggt gggtctcatc acctacatgc
     1681 gtaccgatag cgtccggctt tcacccgatg cggtagacga atgccgtgca tacgtcgcga
     1741 agaagtacgg aaaggattcg gttccggaga agccgaactt cttcaagacc aagaagcaaa
     1801 acgttcagga cgcccacgag gcgattcgtc cgacgcggct cgaccttccg cccgaggccg
     1861 tgagcaaata cctcaacgat ccgcaacgca ggctctacaa gctcatctgg gatcgctttg
     1921 tcgcctcgca aatgacgccg gcggtcttcg atcagacggg agtcgagatc gaagccagca
     1981 gggagggacg cgtctacggg cttcgggcca gtggaagcgt gctcaaggtg cccggatggc
     2041 gcgccgtgta tggagcgacg accgaaccgc tcgccggtga agaaccgagc cccgaagacc
     2101 gcaccgacga cgacgatagc ctgccggagc tccgcgaagg cgaagcgctt cagatcgtga
     2161 ttccgccagg cgtcgaaggg caccacaaga cgaccgagcc accgcctctt ttcaccgagg
     2221 cgacgctggt caagaagctc gaagaagaag gcatcggccg tccgagcacc tacgccgaga
     2281 tcatcagcaa ggtcgagcag cgcgcgtacg tcgaaaagga cggaaacaag ctcgtgccga
     2341 cgcccctcgg aaccttggtc atcgagaagc tggtcgccga caacttcgac ctcgcagaca
     2401 tcggtttcac ccggaagctc gaagaggacc tcgacgccgt cggtgatgcg cgcgcgaagc
     2461 gtctcgatgt gcttcaaccg ttccacgatc ggctccaggc gcagattgcg catgctgtcg
     2521 agcagaaggg caagtggtgg cccgaccccg tcgagatcgg cgagaactgc cccgagtccg
     2581 gcgacccact ggtgcgccgc tggggccgca acgggccgtt catcggttgc acgaagtacc
     2641 cggagtgccg ctacaccctc aacatcggtc cggatggcga gatcgaggca cccaaagagc
     2701 cggagctgac ggagtacgat tgtcacgagt gcggcgcgaa gatgtcgaag cgctggggcc
     2761 gcaacggctg gttcctagga tgctcacgct accctgagtg caagcacact cgcgccgtgc
     2821 ccctcggttt caagtgcccg aagtgcaaga cgggcgacat cgtgccgatc aagcccaagg
     2881 gtaggggacg tccgttctac ggctgtacca actaccgctc cgaagagccc tgcgactttc
     2941 gcgtttggca gaagcccatc ggaatgaaat gcccgaactg cggcagcgac ttcctggtcg
     3001 cgatgggtgg caagaagaac cccaagctca agtgcgcgaa cgagacatgc gacttcgagc
     3061 gcccttacga tccgtccgag ctcgccgatg acggtgagtc cgaagaggtc tcgcctgcgg
     3121 cagattcggc cacgggctga ggttcatcgt ggctgatgtg gtcgtcattg gcgctggcct
     3181 agcgggcacc gagtgtgcct ggcaattggc gcaacgcggc gggcacgtgc gtttgatcga
     3241 gcagaagcct tcgtcgcgca cgcccgcaca gcactccgat caaatggcgg agctcgtctg
     3301 ctcgaactcg tttcgcggcg cggcattgca caacgcggtc gggctgctca aggaagagat
     3361 gcgccgctgc ggctccctcg taatggaggt cggagagctt caccgagtgc cggcgggcgg
     3421 agcgtttgcc gtcgaccgcg accagttcag tgctgagatc acgcggcgcg tggaaaccca
     3481 tccacttatt gaagtggttc atgaagtcgt cgatcggatt cccgaagaga gaccggtcgt
     3541 catcgcgacc gggccgctca catctggggg gcttgcggat gacatcgccg ctgcggtcgg
     3601 cgccgagcac ctcgcctact acgatgcgat cgcgccgatc gtgtcggccg actcgatcaa
     3661 cttcgagcgc gccttccggg catcccgcta cgacaaggga ggggatgacg cctacgtgaa
     3721 ctgcccactc aatcaggatg agtacgaggc cttcgtgcag gcgctgctcg acgcggagaa
     3781 ggtgacccca cgtgatttcg agaaggtccg ctacttcgaa ggctgtctcc ccgtcgaggt
     3841 gatggctgcg cgaggaccca agaccctgtc gttcggctgc atgaagccag tcgggctcac
     3901 cgacccgcgc actggacgct ggcccaaggc cgtcgttcag ctgcgacaag aagacgaggc
     3961 gggcacggcg ttcaacatgg tcggctttca aacgcgaatg acctggcccg agcagaagcg
     4021 cgtgtttcgc atgatccccg ggctcgagaa cgcggagttc gagagactcg gcagcgtgca
     4081 caggaacact ttcgtcaact cgccggtcgt gctcgacgat gacttgtcgc tccgttcgcg
     4141 ccccggagtc tacctcgcgg gtcagatcag tggcgtcgag ggttacgtgg agagcgccgc
     4201 ctgcgggatg ctgctcggta tcaagctggc gcacgagctg cgcgggcggg tcttttcgtt
     4261 tcctccgggg accacgatgc tcggaggcat gatcgggcat ctcagggagc cgaacgacga
     4321 ttttcaaccg tccaacgtga tgttcagcat ggtgcctacg gtcggagaac cacgactggg
     4381 caagcgggcg cgtcgagacg cgcagtcaga gcgcgccttg caggatctcg gggtctggct
     4441 ttcgagcgtg gtagagtcga acgatgccct cgacggatcc gctcgaacag cagatcgacg
     4501 ccttcgctct gtacctagcg gatgagcggc gaagctcggc gcgcacggtc gaaacgtaca
     4561 ttcgcgacct acgcagcttt cgcgactacg tgctcgatca gggcttcacg cccgacgcgc
     4621 gcgagctcga catcgttgcg ctgaggggct ttctgtcgag cttgttccgt tcgaatcagg
     4681 cgagcacgat gaagaagaag gtgagcgcaa tccgctcgtt cttcaagttc ctgctcaagc
     4741 ggcagatggt gaatcagaat ccggctgccg gcttgcgatc gcccaagatc gcgaagacct
     4801 tgccgcggtt cctcagcgtc gatcaggcgt ttcgcgtcat ggaagccccg ccgaaagagg
     4861 acaaacgggc
//
LOCUS       DASRZF010000399         2082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_5109296, whole genome shotgun sequence.
ACCESSION   DASRZF010000399 DASRZF010000000
VERSION     DASRZF010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5109296"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1012
                     /locus_tag="VFG22_17690"
     CDS             <1..1012
                     /locus_tag="VFG22_17690"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET6418133.1"
                     /translation="EITQTKRLSVDAEPLVDHGPSTGDTLRFSTPPTARHLTLELVIS
                     DAELRDRPEPSGEDASETPSAEYLAQLPSMSLFAEVPAAVLTRIVSESSLVDLEPGQN
                     LIDRGTTADALFALVEGSVQVIRTADVDAIVLSEGDVVGISCLLDRVCYEGDATARTK
                     VRALRISKLLLDRLVAEHPALEDILLEMLGRRLVATLVRTSPIFASFDDETRLKVAGM
                     FEVRRAHQGTVILKAGKRADGLYIPMIGALTAIDANGAEMGSLKLGRALGQHSMLTRT
                     PSPLTVKAATDVLLLRMSARRFHSLVAAHPTMVAHLEELARHPSAPAFSLIPEPQLKS
                     GA"
     gene            1065..>2082
                     /gene="thrC"
                     /locus_tag="VFG22_17695"
     CDS             1065..>2082
                     /gene="thrC"
                     /locus_tag="VFG22_17695"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET6418134.1"
                     /translation="MSYSASFRCVAGCPGTYPLNQVIYSCPSCGELLEVHHDMQALKD
                     RSGSAWIKLFDQRWMTRDWPYGSGVWGKKEWVQPNLREENVVSTAEGGTNLFWAERYG
                     KTLGLDEVWIKNCGNSHTGSFKDLGMTVLVSTVKQMIEDGTPIRAIACASTGDTSAAL
                     AAYCASAGIPSVVLLPKGKVTTAQLVQPLANGALVCALDTDFDGCMEIVQKLTTEQGI
                     YLANSMNSLRLEGQKTVAIEIVQQFDWKVPDWVIIPGGNLGNVAALAAGFDMMFDLGL
                     TSKRPRICLAQAEKANPLYRAYKQGWDKFEPMTAGDTEASAIRIGNPVSVRRAIRALQ
                     AFDGV"
BASE COUNT          395 a          662 c          645 g          380 t
ORIGIN      
        1 tgagatcaca caaacgaagc gcctctcagt cgacgcggag cccctggtcg accacggacc
       61 ctcgacaggg gacacgctga ggttctcaac ccccccgacg gcgcgacacc tcacgctcga
      121 gctcgtcatt tcggacgcgg agctccgaga ccgccccgag ccctcgggcg aagatgcgag
      181 cgagaccccc tccgccgagt accttgcgca acttccgtcg atgtccctgt tcgcggaagt
      241 tccggcggcg gttctcacga ggatcgtcag cgagtcgagc ctggtcgatc tcgaacccgg
      301 tcagaacctc atcgacagag gcacaacggc cgacgccctt tttgctctcg tagaaggaag
      361 cgttcaggtg atccgcactg cggatgtcga cgccatcgtc ctatcggaag gcgacgtggt
      421 cggcatctcc tgcctgctcg atcgggtttg ctacgaaggc gacgcgaccg ctcgcacgaa
      481 ggtgcgcgcc ctcaggatca gcaagctctt actcgatcga ctcgtcgcag agcaccccgc
      541 gctcgaagac atcttgctcg agatgctcgg ccggcgtctg gtcgcgaccc tggttcggac
      601 atcaccgatc ttcgccagct tcgacgacga gactcgtttg aaggtcgccg gcatgttcga
      661 ggtccggcgc gcacaccagg gaacggtgat actaaaggcg ggaaagagag ccgatggtct
      721 ctacatcccc atgatcgggg cactgacggc gatcgacgcg aatggcgcag aaatgggtag
      781 cctcaagctc gggcgcgcgc tcgggcaaca ttcgatgctg acccgaaccc cctcgcctct
      841 gaccgtgaaa gcggcgactg atgtcctttt gctgcggatg tcggcccgcc gctttcattc
      901 gctcgtcgct gcgcacccga cgatggtcgc gcacctcgag gagctcgcgc ggcatcccag
      961 cgcaccagcc ttttccctca tccctgagcc gcaactcaaa agcggcgcct gagaccccag
     1021 tggcgatgtg cgctccttga cggagcgcct atactgatgc cctcatgagt tacagcgcct
     1081 catttcgttg cgtcgccggg tgtccgggca cctaccccct caaccaggtc atctactcgt
     1141 gccccagctg cggtgagctt ctcgaagtcc atcacgacat gcaggccttg aaggaccgca
     1201 gcggctcggc gtggatcaag ttgttcgatc agcgctggat gacgcgcgac tggccctacg
     1261 gtagcggcgt ttggggtaag aaggaatggg tccagcccaa cctgcgcgaa gagaacgtgg
     1321 tgtcgaccgc agagggtggc acgaacctct tctgggcgga gcgctatggc aagacgctcg
     1381 gtctcgacga ggtttggatc aagaactgcg gcaacagtca cacgggttcg ttcaaggacc
     1441 tcgggatgac ggttctggtg agcacggtca aacagatgat agaagatggg acgccgatac
     1501 gcgcgatcgc gtgcgcatcc acgggcgaca cctcggcagc gcttgctgca tactgtgcat
     1561 cggctgggat cccttccgtc gttctgttgc cgaaggggaa agtcacgacc gctcagctgg
     1621 tgcagccctt ggcgaacggt gccctcgtgt gtgcgctcga taccgacttc gacggttgca
     1681 tggagatcgt ccagaagctg accaccgagc aagggatcta tctcgcgaac tccatgaact
     1741 cgctccgcct cgaaggacag aagacggtcg ccatcgagat cgtgcagcag ttcgattgga
     1801 aggttcctga ttgggtgatc atccccggcg gtaatctcgg caacgtcgcg gcgctcgcgg
     1861 cgggcttcga catgatgttt gacctcgggc tcacctccaa gcgcccgcgg atttgcctcg
     1921 cgcaggcgga aaaggcgaat ccgctctacc gagcatacaa gcagggctgg gacaagttcg
     1981 agccgatgac cgcgggcgac accgaagcca gcgcgattcg gattggcaac ccggtgagtg
     2041 tgcgccgtgc gattcgcgcg cttcaggcct tcgacggcgt gg
//
LOCUS       DASRZF010000400        10231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3398748, whole genome shotgun sequence.
ACCESSION   DASRZF010000400 DASRZF010000000
VERSION     DASRZF010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 10231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10231
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3398748"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1299)
                     /locus_tag="VFG22_17700"
     CDS             complement(<1..1299)
                     /locus_tag="VFG22_17700"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6418135.1"
                     /translation="MTIAFDEFELDSAKRELRVAGRVRVLQPRVFDLLLYLVENHERV
                     VPKRELLDTLWPDSIVTESSIQRAVSLARSALGERGAELIQTFPRQGYRFIGRIRVSD
                     KPNAKQSLKPRYARSGDVHIAYTTLGEGATDIVIVLGWILSVRAMFKHPKTRAAIEAL
                     SEIGRVILFDKRGTGLSDRVKEVPIHEQRMDDLRAVLDACGSKRAIVIGFSEGGALAM
                     LYAATYPKRVHGLVLSGAFARMIRTPDYPYGYTAEEVEKLKSYIRGAWGKGASLRAIA
                     PSQLSDPDFVDWVAFSEQEGTSPGSALDLLEMNTRIDLRALLPTIRVPTVVMQAAQDA
                     MSHPGNGPYLAENIPNAKYIEAPGDDHVFFFGTQHEISNAVRWILSQEPRALDEENPF
                     LSTALVGHTAGAIDDDAWRALVGSFRGRAVPGKLWAYFDGP"
     gene            complement(1358..1939)
                     /locus_tag="VFG22_17705"
     CDS             complement(1358..1939)
                     /locus_tag="VFG22_17705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418136.1"
                     /translation="MNRAGQKVIRQAALVALCCHCLVAAAVPARAGAEEEEGRNALEV
                     GYTHTFQLLRTSPSEAEAGRSENLWGFVVAYERILIPDRLALVIAKPFHFTQDRFDSP
                     LDVFLKVTFPKGHWDPYVGAGVSGNLRLFSGELEQEEGRRVEYTFGVGAIGGCTYLFT
                     PHWGLSLEVGYLYFVNGLTQHGFIDALSGVWTF"
     gene            complement(1943..2305)
                     /locus_tag="VFG22_17710"
     CDS             complement(1943..2305)
                     /locus_tag="VFG22_17710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HET6418137.1"
                     /translation="MAAIITALIVFVILFHLWAMVLEMFLWTKPLGRKTFGLSKEDAE
                     SSKTLAANQGLYNGFLAAGLVLSFLVQNPNTAFTLQVFFLSCVALAGLYGGVTAKMSI
                     LFMQGLPAMIALGLLLLA"
     gene            2385..5078
                     /locus_tag="VFG22_17715"
     CDS             2385..5078
                     /locus_tag="VFG22_17715"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6418138.1"
                     /translation="MPKSSLGLRFSIQIVLIVGCLAAHASGQSAEPDAGVSPLGAEET
                     SAEAASAEEQALAAARERALAEANLAQTETRRLIAERRARLLSVKEGQARYTDELAEK
                     QVELAAALESALAWNRQVRELEDSVPEGRSRGAEADRFYDALVHDLTQARQNLGSALD
                     AVRSEPSAVPLLQAADSDRIPLDIDDGSLDELENELVGAASILQAEETKLRWDRAQTL
                     SRAIVIMNESRLRLLDMLSEARRESLEGFGPEGIAQVKREVEQIILEARFDLLSLPRE
                     ATERYEQLRSAPGPALAGFFQLFLLILGFRWWWKRADSTLEEMRRTWLQKRPQTLVTR
                     GVSTFAWYLRRIRKPLEWLLFFAILARIIARAGQVADTKYLHIVIRWLAVGAFVVQLI
                     DATASRQGFSTESLDKLRFRSLRLVGVSVVVVGLILSLTQTAVGKGAIYAWVASTFWF
                     LAIPIGLILVFWWRETIFERAKARTASRFLQWVASRDHTVLMYPAAVLGGAYLLIEGI
                     VGFFLRKISELAPLRRLLTYQMRRGVQKSTTAIPAERGTEPLPPELLEALGSGPPTAA
                     GLVTSYMSDRVHAMRALVRSDKRAVIAVVGERGLGKTTFLGRVTSDLEPDALCSVQCQ
                     PGGFPVLLAQFARALELPEGSPEEQIIESLRQRAPRVISVDDAQRLVRPLIGGLEDFD
                     HLTRFMRLVSPETRWLIAIGKPAWHYLRRARGDRSAFDQIVDLKPWEEPQLAALIEQR
                     TREAGIDPSFERLVVPRQVQTVPMSDEERTKRDFYRILLDYSDGNPEVSLYWWRASLY
                     RRPPDDTVHVRLFQGPTATQLDDLPSNFYFVLRSVVQLELAIEKDVIACTDLSPAEVA
                     DALRAAWMRGYIEDRGGFYHVDIAWYRAITGILRRKHLLFI"
     gene            5089..6012
                     /locus_tag="VFG22_17720"
     CDS             5089..6012
                     /locus_tag="VFG22_17720"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HET6418139.1"
                     /translation="MSSRKTTALLVAICGLVLVPSFVLAQDPAGEDAILEITQSIRWG
                     GVLTSVFVILGVWIALRFLSNFVEDLGDQFTTRRMLFQRIGTIVQFFVYIVTVVAVIT
                     LSVRFNDKVLTLLGGTAAVAVGFAVRDLVASFIAGIMIMVDQPFQVGDRVAFGGEYGD
                     ITAIGLRSVRLQTLDDSTVTIPNNKFLTDMTSCGNYGAIDMQIVMDFYIGIDQDVLAA
                     RDIVTEAGLTSRYVYLPKPVVVLVKQVIHENYVAVRLQLKAYVLDTQYEKSFETDVNL
                     RVMHAFAERQIGPPAILHRTISGGAVAAAPC"
     gene            6006..7205
                     /locus_tag="VFG22_17725"
     CDS             6006..7205
                     /locus_tag="VFG22_17725"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET6418140.1"
                     /translation="MLKLIAIAMTALSALGCAPRKPALPDDLSSAEDFERYVDSIVAT
                     GDPPGISVAIVRDTGLVYARGFGWADRPRQLRATPETAYQWWSITKVFTATAVLQLAE
                     ARKVELDAPVKRYLDFFEPKNAGDYEPATVRQLLSHSACLNDVGMAILGWVHFDPTER
                     RPQLEFAEEKLREHGKIVCEPGSEGRYSNLGYIVLAALIEKVSGQGYEDYVAEHIFEP
                     LGMKNSNFVYTVAMRGNEAAGSHPKNLMSWFAFKFFIDEKLAIRELIAGRYWFRQLYS
                     DQKGSTGAIGPATDLGRFATALLRGGELDGVRILSRESVELMARPVVRISKGGPRRGM
                     QFGLAWFREDDEGRATLSHRGGGMAFRAMLALHPEEKLGVVVLSNSTYLDGDGGARIA
                     KAAAMAN"
     gene            7272..7646
                     /locus_tag="VFG22_17730"
     CDS             7272..7646
                     /locus_tag="VFG22_17730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418141.1"
                     /translation="MRKSAVYMLALSSFPIRDAFATQLRRVLSIRRIRVLLVFGLSRH
                     PCGRSDSLIRQRMLDAEAPRPDRTALEAMGREWGSMHADFKRAVEDAPDWLWLKFENV
                     DDEVEAIRAGTRPFEIGLYEGF"
     gene            7719..8174
                     /locus_tag="VFG22_17735"
     CDS             7719..8174
                     /locus_tag="VFG22_17735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET6418142.1"
                     /translation="MRFTKESVIKASAQAVFAFHEAPDAFERLQPPWQESEVVQPPTS
                     LEVGTRVILRAKFGPLWQTIVAEHVEYEPGKMFADRMVEGPFPKWLHRHVVTPRGESE
                     CVLTDDIEYELPLGILGRVFGGWFATRNLERLFEFRHRVTREACEGKRT"
     gene            8171..8914
                     /locus_tag="VFG22_17740"
     CDS             8171..8914
                     /locus_tag="VFG22_17740"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET6418143.1"
                     /translation="MNESAAILNTFAVSHFCESARWTLDYKGVDYSEESWAPLLHVTR
                     TWRMKRTYTPILRIDGEVLQESAAICEYIEERFPEPNLIPKEHRDDVLRLADEARSIG
                     PHVRRMAYHAIGQSPEQLEQAWALNLPSREAKIQQLVFPLTRRMAFKAMKIDEAHAKK
                     SEQFVREYLADRDTWFATGRKYLVGERFTLADLTLAAMLSPLARPTEHPFYPRMDLGA
                     ASEALIESFRGYRLLDWVRRCYAEHRARR"
     gene            9030..>10231
                     /locus_tag="VFG22_17745"
     CDS             9030..>10231
                     /locus_tag="VFG22_17745"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HET6418144.1"
                     /translation="MSEEQFDFIIVGAGSAGCCLANRLSADSNNRVLLLEAGGKDNNP
                     LIKMPIGFTQLMYDARTTNLYTTEPEPHMNGREMSVVRGRVLGGCSSINGMVYMRGQR
                     QDYDDWAALPGCRGWSYANMLPYFKKSENFEPGPANEYHAKGGELNVTHVQMKYDISE
                     AYIKAAASVGIPRNEDINGEKQEGIGYVQVNMKGGQRWSSADAFLSPEVKKRPNLKIV
                     LKVLAKRVLLDGKKAVGIEYQDAKGNTHRATADREVVLSAGTYNSPPLLELSGIGKPE
                     MLRELGVEVKHALPGVGENLQDHLQLWVQQGVKTKQCLSEDGKFPRVIGAVLKYLFTK
                     KGPLTMPACNVGGFVPNEGAERPIFQIHFTPGAGGMDENGKMVATPDPGVNSTVCIIR
                     PTSRGSVHA"
BASE COUNT         1953 a         3060 c         3201 g         2017 t
ORIGIN      
        1 cgggccgtcg aagtacgccc agagcttccc cggcacggct ctgccgcgaa aggacccgac
       61 cagcgcgcgc cacgcgtcgt cgtcgattgc accggccgta tgacccacga gagcagtgct
      121 caagaatgga ttttcttcgt ccagcgctcg tggttcctgg gagaggatcc agcggacggc
      181 gttcgagatt tcgtgctggg tgccaaagaa gaagacgtgg tcatctcccg gcgcctcgat
      241 gtacttcgca ttggggatgt tttccgctag gtacggcccg tttcccggat gactcatcgc
      301 gtcctgtgcg gcctgcatga cgaccgtcgg cactcgaatc gtgggcagca gtgcgcggag
      361 atcaatgcgc gtattcatct cgagtagatc gagtgcgctc ccagggctcg tgccttcctg
      421 ttcggagaac gccacccagt cgacgaaatc cgggtcgctg agctggcttg gtgcgatcgc
      481 acgcaaggat gcgccttttc cccaggcccc tcgtatgtag ctcttgagct tttccacttc
      541 ttcagctgtg tatccatagg gatagtctgg ggtccgaatc atacgcgcga aggcgccgct
      601 gaggaccaat ccatggacgc gtttcggata cgtggccgcg tacaacatcg ccagcgctcc
      661 gccttcggaa aagccgatga cgatggctcg tttcgatcca caggcgtcga gaaccgcacg
      721 aaggtcatcc atgcgttgct cgtggatcgg gacttccttg actcggtcgg agagccctgt
      781 gcccctcttg tcgaagagaa tcactcggcc gatttccgag agagcttcga tcgccgctcg
      841 cgtcttcgga tgcttgaaca tcgcacgcac cgaaaggatc cagccgagca cgattacgat
      901 atccgtcgcg ccttcaccca atgtcgtgta cgcgatgtga acgtcgccgc ttcgagcgta
      961 tcgaggcttg aggctctgtt tggcgttcgg cttgtccgac acgcggatcc tgccgatgaa
     1021 ccggtatcct tgtcgcggaa acgtttggat gagctcagcg ccacgctcgc ctagcgcgct
     1081 cctcgccagg ctgaccgctc gctgaatcga gctttcggtc acgatggaat cgggccagag
     1141 ggtgtcgaga agctcacgct tcggaacgac tcgctcgtgg ttctcaacga gatagagcaa
     1201 gagatcgaac acccgaggct gcaggacgcg cactctgccc gctacgcgga gctcgcgttt
     1261 tgcagaatcg agctcgaatt cgtcgaaagc gatcgtcatt tcggtcgtcg cttggatcta
     1321 tcacagtcga tgccagccgc agcagtccga atgttcgcta gaatgtccag acgccggaga
     1381 gcgcgtcgat gaacccgtgt tgcgtcaggc cattgacgaa atacaggtag ccgacctcca
     1441 gcgagaggcc ccagtgaggc gtgaaaagat aggtgcatcc ccctatggcg ccgacgccaa
     1501 aggtgtactc gacccgtctg ccctcttctt gttcgagctc cccgctgaac aatcgcaggt
     1561 tgccgctgac ccctgcaccg acgtagggat cccagtgacc cttgggaaag gtcaccttca
     1621 agaagacgtc cagtggagaa tcgaagcggt cttgcgtgaa gtgaaacggc tttgcgatga
     1681 ccaacgccag tctatccgga atgagaatgc gctcgtacgc gaccacgaag ccccagaggt
     1741 tctcgctacg gcccgcttcc gcttcgctcg ggctcgttcg cagaagctga aacgtgtggg
     1801 tgtagccaac ttcgagtgca ttgcggccct cctcttcctc tgcgcccgct cgtgctggaa
     1861 cggctgccgc cacgaggcaa tgacagcaga gggcgaccaa cgccgcttgt cggatcactt
     1921 tctgacctgc gcggttcatc cctcaggcga gcagcaacaa accgagggcg atcatggctg
     1981 ggaggccttg catgaagagg atcgacatct ttgcggtgac ccctccgtag agcccggcaa
     2041 gtgccacgca ggagaggaag aatacctgca gagtgaaagc cgtattggga ttctgaacga
     2101 ggaagctgag caccagcccg gctgcgagga agccattgta gaggccctgg ttggcggcaa
     2161 gggtcttcga cgactcggcg tcttctttgg agaggccgaa ggtctttcgt cccaggggct
     2221 tggtccagag aaacatctcc agcaccatcg cccagaggtg aaataggatg acgaagacga
     2281 tcagagcggt gatgatggcg gccacgaatt gcagagcata ctacttgcgg attccccaaa
     2341 gaaagtgttt gtagcggctg tccgcttttc gcaattcgtc gcctatgccc aaatcaagcc
     2401 ttggacttcg attttcgatc cagatcgtcc tgatcgtggg gtgcctggca gcgcatgcga
     2461 gcggccaatc ggccgagccg gatgcggggg tgtcgccgct tggcgccgag gaaacgagtg
     2521 ccgaagcggc gagcgccgag gagcaagcgc tcgccgcagc gcgtgagcgg gcgctcgcgg
     2581 aggcaaatct ggcgcaaacc gagacgcgcc ggctaatcgc ggaacgacga gctcgcctgt
     2641 tgagtgtgaa agagggccag gctcggtata cggatgagct cgctgagaaa caggtcgagc
     2701 tcgctgccgc gctcgagtcg gcgctcgctt ggaaccgtca agtccgcgag ctcgaagact
     2761 ccgttcccga aggtcggtcc cggggcgctg aggccgacag gttttacgac gcgctcgttc
     2821 acgatttgac gcaggcacgt cagaatttgg ggtcggccct cgacgcggtc cgatccgagc
     2881 cgagcgccgt cccgcttctg caggccgcgg actccgacag aataccactc gacatcgacg
     2941 acggttccct tgacgagctc gaaaacgagc tcgtcggcgc ggcgtccatc ctgcaagccg
     3001 aggagaccaa gcttcgctgg gatcgcgccc aaaccctcag ccgcgccatc gtcatcatga
     3061 acgaatcgcg gttgcgtctc ctcgacatgc tcagtgaggc ccggcgcgaa agcctcgagg
     3121 ggttcggacc cgagggcatc gcgcaggtca agcgcgaggt tgaacagatc atcctcgaag
     3181 cccgattcga cttgctctcc ctaccgcgcg aagcgaccga gcgctacgag cagttacgat
     3241 ccgctcccgg gcctgctctc gccggattct ttcagctttt cctgttgatc ttgggcttcc
     3301 gctggtggtg gaagcgggcc gactccacgc tcgaagagat gcgaaggact tggctgcaaa
     3361 agcggccgca gaccctcgtg acccgaggcg tttcgacgtt tgcttggtat ctgcgcagaa
     3421 tccgaaagcc tctggagtgg ctgctatttt tcgcgatcct ggcccgaatc atcgcccgag
     3481 caggtcaggt cgcggacacg aagtatcttc acatcgtgat ccgctggttg gcggtcggcg
     3541 ccttcgtcgt ccagctgatc gatgccactg caagccggca aggcttctcg accgagtcct
     3601 tggacaagct tcgcttccgc tcgctgcgac tggttggcgt gagcgtcgtc gtggtcggct
     3661 tgattctttc gctgacccag acggcggtgg gaaagggcgc gatctacgcc tgggttgcca
     3721 gcacgttctg gtttctcgcg attcccattg gcttgatttt ggtcttctgg tggagggaga
     3781 ccatcttcga gcgcgcgaag gcccgtacgg cctctcgatt tctccagtgg gtggcgtctc
     3841 gcgaccacac ggtgttgatg tatcccgctg cggtgctcgg cggtgcctac cttctcatcg
     3901 aaggtatcgt cggcttcttc ctacggaaaa tcagcgagct ggcgccgctg cgacggctct
     3961 tgacgtacca aatgcggcgc ggcgttcaga agagcacgac tgcgattccg gccgagcgtg
     4021 ggacggagcc gcttcctcca gagctcttgg aggcccttgg atcggggcct ccgacggcag
     4081 ccggcctcgt cacctcgtac atgtccgacc gtgttcacgc gatgcgtgca ctggttcgct
     4141 ccgacaagcg cgcggtcatt gcagtcgtgg gcgaacgagg actcggcaag acgacttttc
     4201 ttgggcgcgt taccagcgac ctcgaaccgg atgccctttg ctcggtgcag tgtcaaccgg
     4261 gcgggtttcc ggtgctcttg gcgcagtttg cccgtgcgct cgagcttccg gagggatcgc
     4321 ccgaggaaca gattatcgag tcgcttcgac agcgcgcccc gcgagtcatc tcagtggatg
     4381 atgctcagcg gctcgtacgc cccttgatcg gaggccttga agatttcgat cacctgactc
     4441 ggttcatgcg cctcgtcagt cccgaaaccc gttggttgat cgcgattgga aagcccgcct
     4501 ggcactacct tcggagagcg cgcggagacc gttccgcgtt cgatcaaatc gtcgacctga
     4561 aaccatggga ggaaccacag ctcgccgctt tgatcgagca acgcacgcgc gaagcgggaa
     4621 tcgatcccag cttcgagcgg ctggtcgttc ctcgccaagt tcagaccgtg cccatgtctg
     4681 atgaagaacg caccaagcgt gacttctacc gcattcttct ggactactcc gatggcaatc
     4741 cggaggtttc gctctactgg tggagggctt ccctataccg gagaccgcct gatgacaccg
     4801 ttcacgttcg cctgtttcaa gggccaacgg cgacccagct cgatgatctt ccaagtaatt
     4861 tctatttcgt ccttcgcagt gtggtccagc tcgagcttgc gatcgagaaa gacgtcatcg
     4921 catgcaccga cctgagcccg gcagaagtgg ccgatgcctt gcgcgccgcc tggatgcgcg
     4981 ggtacatcga agaccgaggc ggtttctacc acgtcgacat cgcctggtac cgcgcgatca
     5041 ccggtattct tcgcagaaag catcttctgt tcatttgagg tgtccgccat gagctcacgg
     5101 aaaacaactg ctttgctcgt tgccatctgc gggctggttc tagttcccag cttcgtcctg
     5161 gcccaggacc ccgcgggcga ggacgcgatc ctggagatca cccaatccat tcgctggggg
     5221 ggcgtgctga cttcggtctt cgtaatcctc ggcgtctgga tcgcactgag gttcttgagc
     5281 aacttcgtcg aggacctggg agatcagttc acgacgcgcc gaatgctgtt tcagcggatc
     5341 ggcacaatcg tgcagttctt cgtgtacatc gtcacggtcg tcgccgtcat cacgctgagc
     5401 gttcgattca acgacaaggt gcttacgctc ctcggtggca cggccgcggt cgctgtcggc
     5461 tttgcggtgc gtgacctcgt cgcatccttc atcgcgggca tcatgatcat ggtcgaccag
     5521 ccgtttcaag tcggagaccg agtcgcgttt gggggagagt acggtgacat caccgcgatt
     5581 gggcttcgtt ccgtccggct gcagaccttg gacgacagca ccgtcacgat cccgaacaac
     5641 aagttcttga ccgacatgac ctcgtgcggg aactacggcg cgatcgacat gcagatcgtc
     5701 atggatttct atatcgggat cgatcaggat gtgcttgcgg cgcgcgacat cgtcaccgag
     5761 gccgggctca cgagtcgcta cgtttatctg cccaaaccgg tggtggtgct ggtcaagcag
     5821 gtgatccacg agaactacgt cgcggtgcgg ctgcagctca aggcctacgt gctcgacacg
     5881 cagtacgaaa agtcgttcga gaccgacgtc aacctgcgag tgatgcatgc gttcgccgag
     5941 cgccaaatcg gtccgcccgc aattcttcat cgaaccattt ccggtggggc ggttgcggct
     6001 gctccgtgtt gaagttgatt gcaatcgcca tgacggcttt gtcggcgctt ggatgtgctc
     6061 cccgaaagcc tgcgctgccg gacgaccttt cgagcgccga ggacttcgag cgctacgtcg
     6121 actcgatcgt agccacaggc gatccgcccg gtatctcggt cgcaatcgtg cgcgacaccg
     6181 gattggtgta cgcgcgcggc tttggttggg ccgatcggcc gcgccaactt cgggcgacac
     6241 ccgagaccgc gtatcagtgg tggtcgatca ccaaagtctt caccgcgacc gcagtcttgc
     6301 agctcgccga agcgcgcaag gtcgagctcg acgcccccgt caagcgatac ctcgatttct
     6361 tcgaaccaaa gaacgccggg gactatgagc ccgcgacggt tcgacagctc ctcagccaca
     6421 gtgcttgtct gaacgacgtc gggatggcga tcctcggctg ggttcacttc gatccgacgg
     6481 agcgaaggcc gcagttggag ttcgccgaag agaagttgcg cgagcacggg aagatagtct
     6541 gtgagcctgg gtctgagggg cgctactcga atctgggcta catcgttctg gccgctctga
     6601 tcgagaaggt gagcggtcaa ggctacgagg actacgttgc agagcacatt ttcgagccgc
     6661 tcgggatgaa gaacagcaac ttcgtctata cggtcgcgat gcgtgggaac gaagcagcgg
     6721 gttcgcatcc aaagaacctc atgtcctggt tcgcattcaa gttcttcatc gacgagaagt
     6781 tggcgattcg cgagctgatc gctgggcgat actggtttcg gcagctctac tccgatcaga
     6841 aaggctcgac cggcgcgatc gggcccgcga ccgacctcgg ccgattcgcc acggccttgc
     6901 tccggggcgg ggagctcgac ggagtgcgta ttctgagccg cgagagcgta gaactgatgg
     6961 cgcgcccggt cgtgcgcatc agcaaaggtg gacctcgcag agggatgcaa ttcgggctcg
     7021 cttggtttcg cgaggacgac gagggccgcg cgaccctttc tcacaggggt ggcgggatgg
     7081 cgtttcgcgc gatgctagcg ctccatccgg aagagaagct gggcgtggtc gtgctctcca
     7141 acagcaccta cctcgatgga gacggcggcg cgcggatcgc caaggccgcg gccatggcga
     7201 actagttgcg ggggcgagga agagcggaat agcggcttgc tcagtcgctg cgcgaagcgc
     7261 aaaaggtcgg tgtgcgcaag tctgctgtct acatgctggc tctctcgagt ttcccgatcc
     7321 gcgacgcttt cgcgacacaa ctccgtcggg tgctttccat tcgcaggatt cgagtcttgc
     7381 tcgtctttgg actcagccgg cacccctgcg gtcgctcaga tagcttgatt cggcagcgca
     7441 tgctcgatgc ggaggcgccg cgtcccgacc gaacggcgct cgaagccatg ggacgcgaat
     7501 ggggttcgat gcatgctgat ttcaagcgcg ccgtcgaaga tgcaccggat tggctctggc
     7561 tcaagtttga aaacgttgac gacgaggtcg aggcgatccg ggcggggacg cgaccgttcg
     7621 aaatcggcct ctacgagggt ttctagaaga gacgccaccg caatcttcga gcttgctgaa
     7681 agctgtcttg gcgctagagc tacgaaaggc tttgcctcat gagattcacc aaagaaagcg
     7741 tcatcaaagc tagcgcccaa gcggtgtttg ctttccacga agctccggat gccttcgagc
     7801 gactgcaacc gccctggcag gagagcgagg tggttcagcc cccgacgtcg ctcgaagtcg
     7861 gtacgcgagt gatcttgcgg gcgaaatttg gaccgctgtg gcagaccatc gtcgccgagc
     7921 acgtcgagta tgagcccgga aagatgttcg cggaccggat ggtcgaagga ccgtttccaa
     7981 agtggctcca ccggcacgtc gtgacgcctc gtggtgagtc ggagtgcgtg ctcaccgacg
     8041 acatcgagta cgagctcccc cttggcatcc tgggtcgggt gttcggggga tggttcgcaa
     8101 cgcgcaacct cgagcgtctc ttcgagtttc gtcatcgggt gacgcgcgag gcctgtgaag
     8161 gaaagcggac ttgaacgaat ccgcggccat cctcaacacc ttcgcggttt cgcatttctg
     8221 cgagagcgcg cgatggacgc tcgactacaa aggcgtcgat tacagcgagg agtcctgggc
     8281 gcccttgctg cacgtcactc gaacctggcg catgaagcgg acgtatacgc cgatcctgcg
     8341 gatcgatggc gaggttctcc aggagagcgc tgccatttgc gaatacatag aggagcgctt
     8401 ccccgagccg aatctgatac cgaaggagca tcgagatgac gtgcttcgat tggcagacga
     8461 ggcgcggagc atcgggccgc atgtgagacg gatggcgtac catgccatcg gccagagccc
     8521 tgagcagctc gagcaggcct gggcgctcaa cttgccgagc cgcgaggcaa agattcagca
     8581 gttggtcttc ccgctcaccc gtcggatggc attcaaggcg atgaagatcg acgaggctca
     8641 tgcgaagaag agcgagcagt tcgtccggga gtatctcgcc gatcgagaca cctggtttgc
     8701 gactggcagg aagtacctgg tcggcgagag gttcacgctt gccgacctga ccctcgctgc
     8761 aatgctttcg ccgctcgcac gcccgacgga acatccgttt tatccacgca tggacttggg
     8821 cgccgcatcg gaggcgctga tcgaatcgtt tcgaggctat cggctgctcg attgggtacg
     8881 gcgttgctac gccgagcatc gcgcccgccg ctgagggccc gctcattacg ccgggcgcca
     8941 agctagacca ggatcgataa ttctcggccg tcggcgctgc ctgtgatagg aacccagggc
     9001 tttcttgccc tcggagcgaa tgagaagaca tgtccgaaga acagtttgat ttcatcatcg
     9061 taggcgccgg gtccgccggc tgctgtctcg ccaaccgtct ttccgccgac tcgaacaacc
     9121 gggtcttgct gctcgaggcg ggcggcaagg acaacaaccc gctcatcaag atgcccatcg
     9181 gcttcacgca gctgatgtac gacgcaagaa cgaccaacct ttacaccacg gagcccgagc
     9241 cccacatgaa cggccgggag atgagcgtcg tgcgtggtcg cgtcctcggc ggatgcagct
     9301 cgatcaacgg catggtctac atgcgcggtc agcggcagga ctacgacgac tgggccgcgc
     9361 tccccggatg ccggggctgg tcgtacgcga acatgcttcc gtacttcaag aagtccgaga
     9421 acttcgagcc cgggccggcc aacgaatacc acgccaaggg cggcgagctg aacgtcacgc
     9481 acgtccagat gaagtacgac atcagtgagg cgtacatcaa agcggcggcg agcgtgggca
     9541 tcccgcgcaa cgaggacatc aacggcgaga agcaagaggg catcggctac gtgcaggtga
     9601 acatgaaggg cggccagcgc tggagctcgg cggacgcctt tttgagcccc gaggtcaaga
     9661 agcggccgaa cctgaagatc gtcctcaagg tgctggcgaa gcgcgtcctt ctcgacggaa
     9721 agaaggcggt tggaatcgag tatcaggatg caaaaggaaa cacgcatcgg gcgacggcgg
     9781 atcgcgaggt ggtgctctcc gccgggacat acaactcgcc gccactgctc gagctttcgg
     9841 ggattgggaa gcccgagatg ctccgggagc tcggcgtcga ggtaaagcac gcgctccccg
     9901 gcgtcgggga gaatttgcaa gatcatcttc agctttgggt tcaacagggc gtcaaaacga
     9961 agcagtgcct gtctgaggac gggaagttcc ctcgtgtaat cggggccgtg cttaagtacc
    10021 tcttcaccaa gaaggggccg ctcacgatgc cggcctgcaa cgtgggcggt ttcgtcccga
    10081 acgagggcgc cgagcgaccg atctttcaga tccacttcac gccgggtgcg ggcggcatgg
    10141 acgagaacgg caagatggtc gcaaccccgg accccggtgt taattccacc gtgtgcatca
    10201 ttcgcccgac ctcgcgcggc agcgtccacg c
//
LOCUS       DASRZF010000401         7712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4093374, whole genome shotgun sequence.
ACCESSION   DASRZF010000401 DASRZF010000000
VERSION     DASRZF010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 7712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7712
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4093374"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VFG22_17750"
     CDS             <1..312
                     /locus_tag="VFG22_17750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418145.1"
                     /translation="KGVANVYRETFDMSLRIGVDILRELGLRAYQSQRAAQKFRRHDE
                     LSLIKLAQLRHDRVQYLSAAREQIEYLEKTMLEDLSAPDDFKDTGWDAESLRKELAPK
                     S"
     gene            complement(341..973)
                     /locus_tag="VFG22_17755"
     CDS             complement(341..973)
                     /locus_tag="VFG22_17755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418146.1"
                     /translation="MPSDYAQQALAHLRDPSDFRWYVIPLLVIVLYIYHDQLGRKNYG
                     VVFAGLALWGMDWFNEIWNSLFFHFSGYAPVWAAPGDTAYLILIGLNIEICFMFAILG
                     IVTTVMLPAKEAKVLGLPNRWVFAVFFSALAVFIEYLLNAVNALTWDYSWWNRSAPWL
                     IFLIGYFPFFVVAYWVHDMKCIKRQAAAVGAILGLDALALIVFGGVLGWI"
     gene            1128..2525
                     /locus_tag="VFG22_17760"
     CDS             1128..2525
                     /locus_tag="VFG22_17760"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET6418147.1"
                     /translation="MLRFLSNRWSLLCLAALIAGISGGAVAFGEHASSGQQGDTEIID
                     GERVLRAPGLDGWNARKQSPKNNAGQGDRFGSAFHFDAYVYDKATSSPSVTGVVRRGS
                     VVAVGDKVSGSGCTDGSWYEATPFGYVCTSLGFHVSETPSTNRYGVPPAKVSEHLPYQ
                     YSRVITKRAPRYYRIPTVEEEKQARRAMDKKGSTPEVVSVLMEGDYFLALAEKETRKE
                     DGAVFYRTVRGRFVKEADVELRDPAVVRGEELGRDGWRLPLAIVYGEDRELLDLSGKQ
                     PRVKGKAEKHARFVVQEELSKNGVAYVSGSPGAVRRDEVRVARRTKRPSEVPAKGKWI
                     HVDLAEQTLVAYEGDEAVYATVISSGKEGYEPPTGLFEIQQKYVSTTMNATDPIDGFY
                     EVEEVPWTLYYFGGYALHGAYWHTDFGKVRSHGCTNIAPVDARWLYYWSEPEVPAAWH
                     AVRFQPETTWVEFTR"
     gene            complement(2515..3255)
                     /locus_tag="VFG22_17765"
     CDS             complement(2515..3255)
                     /locus_tag="VFG22_17765"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET6418148.1"
                     /translation="MEALAQIATPEFAAAFVVSFLAAALHSTVGFGFALISVPILSLL
                     NPLFAPVPQLLVVLPLTLAIVFRERHAVEVRSTLWIFAGRLPGAVLGVVLLKLLSAAA
                     LDVLMSVMVILGVAIVVTHASFRRTSAREFVAGVASGTMAMVSAIGGPPIALLYRNDA
                     GPTVRANLGLVFAVGLCITISVRAAAGEVSWDEVAIGAALLPAVLLGLSVSGVLIPRV
                     EGPRLRNAIVLVAGVAALMLLARGLLSA"
     gene            3286..4200
                     /locus_tag="VFG22_17770"
     CDS             3286..4200
                     /locus_tag="VFG22_17770"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HET6418149.1"
                     /translation="MRPTKMKTSGDPLKKTATRVLRLATSALDTAVRGLDNTRRVANF
                     LRGRLELEIRPSDVFISSYPRSGTTLTQWILYLLTHEEQPDPTHLTEVSPWFERSLAI
                     GELTAADLDRFPSPRIFKSHLPREWLPDGARYIYVERDGLDVLVSYFHFYCAYLGFQG
                     SLEDFHGLFIEGRLQYGSWFEHVAGWRARASDPDVLVVRYEELLNDRKASIEGIVDFL
                     GWTREERWVDRAVIESSFDAMKRRESVFDHATALLLERGVSPRSFLRSGQSGSGADAL
                     TQAQVLEIRERSSKGRRLAIRGLPAFLQ"
     gene            complement(4136..5059)
                     /locus_tag="VFG22_17775"
     CDS             complement(4136..5059)
                     /locus_tag="VFG22_17775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418150.1"
                     /translation="MSSYNGTDSSRGTLASLANRQELFARRQYEPVVSPEALESTTST
                     WFGVGVAADDASCGLPVDVLILVFAQEMVRRELGLAKSSILIADSNACNAGVPRLDVH
                     RAASLAQAPLEALIERFGFPVDIRRSSSLAPASEVERKQEALDIENGYVAQQLVQTEI
                     MRSRGAAVKIGWALSACTYDETYFDRLYRNRFGEGVSFAYTIGGRTLNPQRPRACPYL
                     CREPRDRLLLGTKENAVRKLYSDPGSPAVRGYHRLIGKLARAYNHLTGNNPGTPETFL
                     QFVVDASASPITAGKREARESRAGAPSSFAP"
     gene            5210..6640
                     /locus_tag="VFG22_17780"
     CDS             5210..6640
                     /locus_tag="VFG22_17780"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET6418151.1"
                     /translation="MRNLACFLTLAFLAAFTVSLTPSHAQETPWKRRLRTGVVVDEAP
                     EPAADGPRGALTADEMRRLGLRPRGGGLVDLSYWPDEPGSPARVRPSELAEAMRELCP
                     TSTRSSEIERYAAAIVQYSQEFAIDPWLLAALVYSQSDCKADIENSYGTGLTLLNVGM
                     HAGNIRAGKYYFGVLSHEGWERKELDVSAFELRRDALLSPQANIYFAAALLNVFGQQC
                     PQIDRAFDSVPHRHAVSHFVWGDRVADTAPEDRILLARRRLLNSHAMLQKHGKGLGGI
                     SFVSPLDGYPRIAISGLGEPRDNGHRLHRGIDFASEQGEPVRSIAAGTVTFAGVDWER
                     RAHIPLEPWGAVIVDQRHMGPRGLFVEVDHGNGVVSLYAHLASYDVEIGEQVDAGQRI
                     GEVGRTGIRDSGPHLHFGLFEDGEVRDPLVYLAAYLFPPNLTKRFHVDLASAHRHPNR
                     KQRSLRGTPGRSADRATDRRSPAPHF"
     gene            complement(6573..7205)
                     /locus_tag="VFG22_17785"
     CDS             complement(6573..7205)
                     /locus_tag="VFG22_17785"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HET6418152.1"
                     /translation="MLRSLTLSIVVSSALLCSSCSSSVVQTGTVEVQTDEDADFTEYQ
                     SFSVVTADFVDPDDLPDLSDEQIAFNDQVNDLIVQAMQAEPVCLEFIPPDEVTEENQP
                     DLWAANGLGKSTDGGYYYECVGDWWWGYWGWYWDPCAYWYPVYVEYDVGSLLILAGPP
                     PVGTDEPAPVFAGLAQSVLGTGPDLDTKVKNAVQAIFDQWPDPRSCPASP"
     gene            complement(7251..>7712)
                     /locus_tag="VFG22_17790"
     CDS             complement(7251..>7712)
                     /locus_tag="VFG22_17790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418153.1"
                     /translation="VNVGGGTVSVGTKVGKRKFVPGVNSRPPKGIEDMPPSVLGAFLE
                     TGVPGIHVTQIIDLAEDYGLEIAPHVIPEVGTGDIFQKRQPNRWLAGIVLALILLSLF
                     VIARAPWGTRMLRTTIAPESVLPPPPRAPSALGPRVDPSEVSQPPQSPTPD"
BASE COUNT         1610 a         2254 c         2470 g         1378 t
ORIGIN      
        1 aagggcgtcg cgaacgtcta tcgcgaaacg ttcgatatgt cgcttcgaat aggcgtggat
       61 atactgcgag agctcggcct ccgcgcgtat caatcacagc gagcagcgca gaaattccgt
      121 cggcacgacg agctatcgct gatcaagctt gcccagctac gacacgaccg tgtccaatat
      181 ctcagcgcag cgagggagca gatcgagtac ctggaaaaga cgatgctcga ggacctgagc
      241 gcgcccgacg acttcaagga cacgggctgg gacgcagagt ctctacgaaa agagctcgca
      301 cccaagtctt agcctggagt gtttcaatgg cagccgcggc ctagatccag ccgaggaccc
      361 caccgaagac gatcagggcc agagcgtcta acccgagaat tgcaccgacg gccgcggcct
      421 gccgtttgat gcacttcatg tcgtgcaccc agtaagcgac cacgaagaac gggaagtagc
      481 cgatcaagaa gatcaaccat ggggcggagc ggttccacca ggagtaatcc caggtcagcg
      541 cgttgaccgc gttcagcagg tattcgatga agaccgcgag cgcgctgaag aagacggcga
      601 acacccagcg gttgggaaga ccgagaactt tggcttcctt ggccggaagc atgaccgtgg
      661 tgacgatgcc tagaatcgca aacatgaaac agatctcgat gttcaggccg atcaggatga
      721 ggtacgcggt atcgcccggt gcggcccaga ctggcgcgta gccgctgaaa tgaaagaaca
      781 acgagttcca gatctcgttg aaccagtcca tcccccagag cgcgaggccc gcgaagacaa
      841 cgccgtagtt ctttcggccg agctggtcgt ggtagatgta gagcacgatc accagaagcg
      901 ggatcacgta ccagcgaaag tcggacggat cgcgaaggtg agcgagcgct tgctgagcgt
      961 agtcggaagg catgcgtcgg acttaccacc ggaaattggg caccgcctag tgcataaatt
     1021 gcgccgaaaa tcccaatccg ggccgatctt ttgaatcccg acgaagtggc gctatcctca
     1081 ggcgtcggcg cacggtgggt cgagcgctgg cctcgaacat gcttacgatg ttgcggtttc
     1141 tctccaatcg ttggtccctt ctatgtctgg cagctctgat cgcgggaatc agcggcggtg
     1201 cggttgcgtt cggcgagcac gcatcgagcg gccaacaggg cgacaccgaa atcatcgacg
     1261 gcgagcgcgt gttgcgggcg ccgggtctcg acgggtggaa cgctcggaag caaagcccga
     1321 agaataacgc cggacagggt gaccgcttcg gttcggcctt tcacttcgac gcatacgtct
     1381 acgacaaggc gacgagtagc ccaagcgtca ccggcgtcgt ccgaagaggt tcggtcgtcg
     1441 cagtcggaga caaggtgagc ggctcgggat gcaccgacgg cagttggtac gaagcgactc
     1501 ccttcgggta cgtctgtacg tctctcggct ttcacgtgag tgagacgccg agcacgaatc
     1561 gatatggggt gccaccggcc aaagtgagcg agcaccttcc ctatcagtac tctcgagtca
     1621 tcacgaagag ggcaccccgt tactatcgca ttccgacggt agaagaagag aagcaggccc
     1681 ggcgtgcgat ggacaagaag ggatctacgc ccgaggtcgt atcagtgctg atggaaggcg
     1741 attacttcct cgcgctcgcc gaaaaggaga cccggaaaga agacggggcg gtcttctaca
     1801 gaaccgttcg cgggcggttc gtcaaggaag ccgacgtcga gctccgcgac cccgccgtag
     1861 tgcgaggcga agagctgggt cgggacggtt ggcgactgcc acttgcgatc gtctacggcg
     1921 aggatcgcga gctgctggac ctgagcggca agcagccacg ggtgaagggc aaggccgaga
     1981 agcacgcgcg gtttgtcgtc caagaggagc tcagcaagaa cggggtcgcg tacgtgtcgg
     2041 gatcccccgg cgcggtgcgg cgtgatgaag tgcgtgtggc gcgtcgaacc aagcgaccga
     2101 gcgaggtacc ggccaagggc aagtggattc acgtggacct cgcagagcag accctggttg
     2161 cgtacgaagg cgatgaagcg gtctatgcga ccgtgatctc gagcggcaag gaggggtacg
     2221 agcctccgac gggtctgttc gagattcagc agaaatacgt ctcgaccacg atgaacgcga
     2281 ccgacccaat cgatggcttc tacgaggtcg aagaggtgcc ctggacgctc tactacttcg
     2341 gcggctacgc gcttcacggc gcgtactggc atacggattt cgggaaggta cgaagtcacg
     2401 gctgcaccaa catcgcgccc gtcgatgcac gctggctcta ctactggagc gagccggagg
     2461 tccctgccgc ctggcacgcc gttcgatttc agccggaaac gacctgggtg gagttcacgc
     2521 gctgagcagg ccgcgcgcga gcaacatcaa cgcagcgact ccggcaacga ggacgatggc
     2581 atttcggagg cgcggccctt ctacccgcgg aatgagcact ccgctcacgc tcaaccccag
     2641 tagaaccgcg ggcaagaggg ccgccccgat cgcgacctcg tcccaactga cttcacccgc
     2701 tgcggcgcgc acgctgatcg tgatgcagag tccgacggcg aacacgagtc cgaggttcgc
     2761 gcgcacggtt gggcctgcat cgtttcggta gagcaaagcg atgggcggtc ctccgatcgc
     2821 tgagaccatc gccatggtgc ccgaagccac accggcgacg aactctcgtg cagaggttcg
     2881 acgaaagctc gcatgggtaa cgacaattgc gacgccgagg atcaccatca ccgacatcag
     2941 cacgtcgagc gcagcggccg ataggagctt gaggagcacc acgccaagga ctgccccggg
     3001 gagccgaccc gcaaagatcc aaagggtgct gcgaacctcg acggcatggc gttcgcggaa
     3061 gacgatggcc agggtgagtg gcaacacgac gaggagttgg ggaaccggag cgaacagcgg
     3121 attgagaagc gagagaatgg ggacgctgat gagcgcaaaa ccgaagccga cggtgctgtg
     3181 gagggcggcg gcgaggaagc tcacgacgaa cgctgcggcg aactcaggtg ttgcgatctg
     3241 tgctagggct tccatcgggg atgctagggt agtcagcgtc cgatcatgcg gccaaccaag
     3301 atgaagacga gcggagaccc actcaaaaag acggcgactc gcgtccttcg tcttgccacg
     3361 agcgcactcg atacggcagt gcgtggcctg gacaacacgc gcagggtcgc gaacttccta
     3421 cgcggccgcc tggagctcga aattcgacca agcgacgtct tcatttcgag ctatcctcgc
     3481 tcgggaacca ctctgacaca gtggatcttg taccttctga cgcacgaaga gcagcccgac
     3541 cctacccatc tcaccgaagt ttcgccctgg ttcgaacgct cgctagcaat cggcgagctc
     3601 accgcagcgg acttggatcg gttcccttca ccgcgcattt tcaagagcca tctaccgagg
     3661 gaatggcttc ctgacggtgc gcgctacatc tacgtcgagc gggacgggtt ggatgttctc
     3721 gtttcctact tccatttcta ctgcgcgtat ctgggcttcc aaggctcgct cgaggacttc
     3781 cacggcttgt tcatcgaagg ccgcctgcag tacggctctt ggttcgagca cgtcgctgga
     3841 tggcgggcaa gagcgtctga ccctgacgtt ctggtggttc gctatgaaga gctactgaat
     3901 gatcgaaagg catcgatcga agggatcgtc gatttcctcg gctggacgcg cgaggaacgt
     3961 tgggtcgatc gagcggtgat cgaaagcagc ttcgacgcga tgaaacgacg cgagtcggtg
     4021 ttcgaccatg caacggcgtt actgctcgag cggggagtga gtccccgctc gtttctgcga
     4081 tcgggccaaa gcgggagcgg tgcagacgct ctgacgcagg cgcaagttct agagatcagg
     4141 gagcgaagct cgaaggggcg ccggctcgcg attcgcgggc ttcccgcttt cctgcagtaa
     4201 tcggacttgc agacgcatcc acgacgaact ggaggaaggt ctcaggggta cctgggttgt
     4261 ttccggtcag gtggttgtag gcacgggcaa gcttcccgat caatcggtga tatccgcgaa
     4321 ccgcaggaga gccagggtcg gagtagagct ttcgcacggc gttctctttg gtgcccagga
     4381 gcaagcgatc tcggggctcc cggcagaggt acggacaagc gcgcgggcgc tgtggattga
     4441 gggttcggcc cccgatcgtg taggcgaacg aaaccccctc gccaaatcgg ttgcgataga
     4501 gccgatcgaa gtaagtctcg tcgtaggtgc aggctgaaag ggcccaaccg atcttcaccg
     4561 cggctccacg gctgcgcatg atctctgtct gcaccagttg ctgtgcaacg tagccatttt
     4621 cgatatcgag cgcctcttgc ttacgttcga cttccgatgc cggtgccaat gaagacgagc
     4681 gacgaatgtc cacgggaaag ccaaatcgct cgatgagagc ttccaagggc gcctgcgcga
     4741 gcgaagctgc gcgatgcacg tcaagccttg ggacgccggc attgcacgcg ttgctatccg
     4801 cgatcaggat cgagctcttt gcgagcccga gctcccggcg aaccatttcc tgggcgaaga
     4861 cgagaatcag gacgtcgacc ggaaggccgc aactcgcgtc gtcagccgca acgccgacgc
     4921 cgaaccaggt cgacgtggtc gactcgagcg cttcgggaga gaccacaggc tcgtattgac
     4981 gccgagcgaa gagctcctgg cggtttgcaa gcgatgcgag ggtccccctc gatgaatctg
     5041 tcccgttgta actgctcatc ccacgtttcg ccgcatagcc cgaacggcac cgtagcggac
     5101 atggagcgag tcggcaaaac ctttgaagac acgaaacagt ccggttactt acgtcattaa
     5161 gttggaggct tcgattcttg gctgcatgct tagagcttcg gagagacgct tgagaaatct
     5221 cgcctgtttt ttaaccctgg ccttcctcgc tgcgttcacc gtttccttga cgcccagcca
     5281 tgcgcaagag acgccctgga agaggaggct ccggacgggg gtcgtggtgg acgaagctcc
     5341 cgagccggcc gccgatggac cgaggggtgc gctcacggcc gatgagatgc ggcgcctggg
     5401 gcttcgcccg cgcggaggag gtctcgtcga cttgtcgtac tggcccgatg agcctgggtc
     5461 acccgcccga gtgcggccta gcgagcttgc tgaggcgatg agagaactct gtccgacctc
     5521 gacgcgatcg agcgagatcg agcgctacgc tgcagcgatc gttcagtaca gtcaggaatt
     5581 cgccatcgac ccatggcttc ttgctgcact cgtatactca caaagcgact gcaaggcaga
     5641 catcgagaat agttacggta ctggactgac gctgctcaac gtcggaatgc atgcgggcaa
     5701 cattcgcgcc ggcaagtact acttcggtgt cctcagccat gaaggttggg agcgcaaaga
     5761 gctggacgtg agcgccttcg agctacgtcg agacgcgctg ctgagcccgc aagccaacat
     5821 ctattttgct gccgcgttat tgaacgtttt cggtcagcag tgtccgcaaa tcgaccgcgc
     5881 gttcgactcg gtgcctcatc gccatgccgt ctcccatttc gtgtggggag accgggttgc
     5941 cgacacggcg ccagaggacc gcattctgct tgcgaggcgc cgtctgctca acagtcatgc
     6001 aatgctccag aagcatggaa aagggctcgg ggggatctcc ttcgtgagcc cactcgatgg
     6061 ctacccgcga atcgcaatca gcggtctggg cgagcctcgc gacaatgggc atcgcctgca
     6121 ccgaggtatc gacttcgcgt ccgaacaagg tgagcccgtt cgttcgatcg cggcggggac
     6181 cgtcacgttc gctggcgtcg attgggagcg ccgagcgcac atcccattgg aaccttgggg
     6241 cgccgtgatc gtggaccaac gccacatggg acctcgggga ttattcgtgg aagtcgacca
     6301 cggaaacggc gtggtttccc tctacgcaca tctcgcgagc tacgacgtgg agatcggcga
     6361 gcaagtggac gccggccagc gtataggcga ggtgggtcgc acgggaatca gggactctgg
     6421 accgcacctg cacttcgggc tcttcgagga cggcgaggtc agagacccat tggtgtactt
     6481 ggcagcctat ctcttcccac caaacctcac gaagcgcttt catgtcgact tggcgagcgc
     6541 acaccgacac ccaaaccgca agcagcgttc cctcaggggg acgccgggca ggagcgcgga
     6601 tcgggccact gatcgaagat cgcctgcacc gcatttttga ccttggtatc gaggtctgga
     6661 ccggtaccaa ggaccgactg agcaaggcct gcgaagactg gagcaggttc gtcggtgcca
     6721 accggtggcg gaccagcgag gatgaggaga ctgccgacgt cgtactcgac gtacaccgga
     6781 taccagtatg cgcaggggtc ccagtaccaa ccccagtagc cccaccacca atcgccgacg
     6841 cactcgtagt agtagccgcc atcggtcgat tttccgaggc cgttggctgc ccagaggtcg
     6901 ggctgattct cttcggtcac ctcatcgggc gggatgaact ccaaacacac tggttccgct
     6961 tgcatggcct ggacgatcaa gtcattgacc tggtcgttga acgcgatttg ctcgtcactg
     7021 agatccggaa gatcgtcggg atcgacgaag tccgcggtga cgacgctgaa gctctggtac
     7081 tcggtgaagt ccgcatcttc gtcggtttgg acctctacgg taccggtttg gacgacgctg
     7141 ctcgagcaac tcgagcaaag aagcgctgac gacacgacaa tggacagagt gaggctacga
     7201 agcatttgaa ccctccaagt gcgatcggga tagcacgctg gggttgttca tcaatccggg
     7261 gtgggacttt gcggcggctg actgacctcg cttgggtcga ctcgtggccc aagcgcactc
     7321 ggcgcgcggg gcggaggcgg cagaacgctc tcaggcgcga tcgtcgtgcg aagcatgcgg
     7381 gtaccccagg gggcccgggc gatcacgaag agggagagca ggatcaaggc cagcactatg
     7441 ccagcaagcc agcgattggg ttggcgcttc tggaagatgt cccccgttcc cacctcgggg
     7501 atgacatggg gggcgatttc caggccgtag tcttcagcca ggtcgatgat ctgtgtcacg
     7561 tgaatccccg gcaccccggt ctccagaaac gctccgagca ccgaaggggg catgtcctcg
     7621 atgcccttgg gcggcctgga attgacgccc ggaacgaact tgcgcttgcc gactttcgtt
     7681 ccgacggaga ccgttccgcc gccgacgttg ac
//
LOCUS       DASRZF010000402         4487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3054989, whole genome shotgun sequence.
ACCESSION   DASRZF010000402 DASRZF010000000
VERSION     DASRZF010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 4487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4487
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3054989"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VFG22_17795"
     CDS             complement(<1..706)
                     /locus_tag="VFG22_17795"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HET6418154.1"
                     /translation="MELSTIVLAVAAVAGLYMAWNIGANDVANAMGTSVGSGALTLRR
                     AIIVAAIFEFGGAMLVGGTVTQTIRKGIVDVEAFGSDPMVIAIGMTCCLLAAALWLNI
                     ATYAGWPVSTTHSIVGAVVGFGLVAGGFDAVNWGVMVKIVASWVVSPLMGGLLGYLGF
                     VFIKRRILNVDRPLLALRGWGPIMVFPIFAILALSVLFKGLKPLNLDLGFGPAMGIAA
                     ATGLLASFISVPLLRKF"
     gene            complement(717..1391)
                     /locus_tag="VFG22_17800"
     CDS             complement(717..1391)
                     /locus_tag="VFG22_17800"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00153 family protein"
                     /protein_id="HET6418155.1"
                     /translation="MRIPLANLLARNPLPKVGDLMAEVLKTSDRIPELIRLLAKGDQA
                     GVERLAKEISAMEGKADDAKNAARSKMPVRLLMPVDRRDVLKLISQIDAIADCAEDVG
                     VLLTIRPLTVPDGMKPVLELFVERVLDTIRESAKLIDLIDDLVESGFSGPPAERVLEQ
                     AAILGRAEHEADKIQDRCAKALFKAEDSMPPAALFMWTKVLNKIGDMANHAENIGDQF
                     RLFVAG"
     gene            1585..2274
                     /locus_tag="VFG22_17805"
     CDS             1585..2274
                     /locus_tag="VFG22_17805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6418156.1"
                     /translation="MGSRILVVEDEIDLAELVAFNLRSAGHDVTVAHDGHTALAEIQR
                     GQPDLLVLDVMLPDLSGIEVCRRLRRNPQTVRLPVIMLTARSDEVDRVVGFEVGADDY
                     VPKPFSPRELVLRIEAILRRTSAVPAFEGPQLITLGDLSIDVPAHRVEVQGEEILLTA
                     LEFRLLLDLASRAGRVQSRDALLERVWGYSPNVETRTVDTHVKRLREKLGAGSKQIET
                     VRGVGYRALKS"
     gene            2311..3681
                     /locus_tag="VFG22_17810"
     CDS             2311..3681
                     /locus_tag="VFG22_17810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6418157.1"
                     /translation="MIQRYTLLGGVIALVMIALQAFWQGAAPGLPGWIAAMTALLIAC
                     LIGWAAARRDSRDLRRLQRQVAALSDRDFGKRTRMQRPDAIGELGRSLDALSERLSTS
                     EIKKQGRKDRLQTIVDAMAEAVLVTDAEGRIKLSNEVLADLVGFDPTGKTAIEAIRHP
                     DFHRAIEDAQQGFSGLHEIEINGLNARSRHILRASVSPLRNQQGVVAVFHDVTAERAA
                     AQVRRDFVANAGHELRTPLTAIRGFAETLRDGALDDPKAAMGFIEVILRHTRRLQALV
                     DDLADLSSFEGKELELDLVPVNAGAALSEVVRGLEAQSKAKGLKVNLTGLDDAPLVLA
                     EERALEQVLVNLVDNAIKYTPERGEIRIDIAPEGRDVFIEVANSGPGIPARHLSRVFE
                     RFYRVDAGRSRELGGTGLGLSIVKHLVAKMRGEINVDSSAGWTRFQLCLPLADSPNSD
                     TSVSRN"
     gene            3708..>4487
                     /locus_tag="VFG22_17815"
     CDS             3708..>4487
                     /locus_tag="VFG22_17815"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HET6418158.1"
                     /translation="MTPARFICHALVALGLTSTVLAQEDPSSTETGQTQQVVVEPPAA
                     DAMADVVEPTGDMTGDMAEPTEDVAEPLQAETPEVTEVVDAPPPEAETTHEGRRSAEE
                     LRPKREDEMMGDHPVGPGGIKFRPGTGLEINSEDDDFQLRIRVRVQFLYTLVKGNLGP
                     READEPEDLRNDFRIRRARFIFQGHAFGKNNKYKFELDPLRPDNVVLDYYLDFTQNRN
                     IEVRVGQYKISSNRQRVISSGNLQMVDRSAVNAEFSLDRDIG"
BASE COUNT          899 a         1376 c         1383 g          829 t
ORIGIN      
        1 tgaacttgcg gagcagcggg accgagatga agctcgccaa gaggcccgtc gcggcggcga
       61 tccccatcgc gggaccgaag ccgagatcga gattgagcgg cttgagccct ttgaacaaga
      121 cgctcagcgc gaggatcgcg aagatcggga aaaccatgat cgggccccaa cccctcagcg
      181 cgagcaaggg acggtcgacg ttgaggatgc ggcgcttgat gaagacgaag ccgaggtacc
      241 cgaggaggcc gcccatcagt ggggagacca cccagctggc gacgatcttg accatgacgc
      301 cccagttcac ggcatcgaaa cctcccgcga ccaagccgaa gcccacgacg gccccgacga
      361 tgctgtgcgt ggtcgaaacc ggccagcctg cgtacgttgc gatgttgagc cagagcgcgg
      421 cggcgagaag gcagcaggtc atgccgatcg cgatcaccat cgggtcgctg ccgaacgcct
      481 cgacgtcgac gatgcccttc cggatggttt gggtgacggt gcctccgacc agcatcgcac
      541 cgccaaactc gaagattgca gccacgatga tggcccgcct cagcgtgagc gcgccggagc
      601 ccacggaggt tcccatggcg ttcgccacgt cgttggctcc gatgttccag gccatgtaga
      661 ggcccgcgac ggccgctacg gccagtacga tcgtgcttag ctccatgaga accagcctag
      721 cccgctacga aaagtctgaa ctgatccccg atgttttcag cgtggttcgc catgtcgcca
      781 atcttgttga ggaccttggt ccacatgaac agcgcggcgg gcggcatgga atcctctgcc
      841 ttgaagaggg ccttcgcgca tcgatcttgg atcttgtcgg cctcgtgctc tgcccggccc
      901 aagatcgcag cctgttcgag cacacgctcg gctggcgggc cggagaatcc cgattccacg
      961 agatcgtcga tgaggtcgat cagcttggcc gactcgcgaa tcgtatccag cacccgctcg
     1021 acgaagagct cgagaacggg tttcattcca tcgggtacgg tcagcggacg aatcgtcaga
     1081 aggaccccga catcctcggc gcagtccgcg atggcgtcga tctgactgat cagcttgagc
     1141 acgtctcgcc gatccacagg catcaatagc cggaccggca ttttggagcg ggctgcgttc
     1201 ttcgcgtcgt ccgccttccc ttccatcgca ctgatctcct tggccaagcg ctcgacgcct
     1261 gcttggtcac ccttggcgag cagcctgatc agctccggaa ttcggtcgct cgtcttgagc
     1321 acctcagcca tcaggtcgcc cactttgggc aaggggttgc gcgcaagcag gttcgcaagt
     1381 ggtattctca tggcacctcg gggccaagcc tctcatccct tttggtgccg ctcaaatgct
     1441 aagtccaaag ggctgattcc ctcgtgtttc gtcctggttc cgcgttgagc ccaggggcgc
     1501 tccgtgtaga ctgctggttg ctcgcttgga accagcccgt cacagggacg tcacaagagg
     1561 tgcgcaggct acggttatcg caaaatgggt tcgagaatcc tagtcgtcga ggacgagatt
     1621 gatctggcag agctggttgc gttcaacctg cggtccgccg gccacgacgt cacggtcgcc
     1681 catgatggtc acacggctct cgccgagatc caacgtggtc agccggactt gttggtgctc
     1741 gatgtcatgt tgcccgatct ctccggcatc gaggtatgtc gacgacttcg gcgcaaccct
     1801 caaaccgttc gattgcccgt gatcatgctc acggcgcgaa gcgacgaagt agaccgcgtg
     1861 gtcggcttcg aagtgggagc cgacgactat gtgcccaagc ccttcagccc gagagagctc
     1921 gtactgagaa tcgaggcgat tcttcggcgt accagtgccg tgccagcgtt cgaaggtccc
     1981 cagctgatca ccttgggcga cttgagcatc gacgttcccg cgcatcgcgt cgaggtacaa
     2041 ggcgaagaaa ttctactcac cgctctcgag ttccgattgc tgctcgatct ggcgagccgt
     2101 gcaggccgcg ttcaatcgag ggacgcgctt ctcgagcgcg tttggggcta ctcaccgaac
     2161 gtcgagacga ggaccgtcga cacccacgtc aagaggctac gcgagaagct cggagcgggg
     2221 tccaagcaga tcgagacagt tcgcggagtt ggttataggg cgctcaagtc ctgagttggt
     2281 ccgggcccgg gacccgcgta ttcttagatc gtgattcaac ggtacacact gcttggcggg
     2341 gtcatcgctc tggtgatgat cgcccttcaa gcattttggc agggtgctgc gcctggcctg
     2401 cctggctgga tcgcggcgat gaccgcgctt ctgatcgcct gcctcatcgg ctgggcggct
     2461 gcgcgccgag attcccggga cctccggcgc cttcaacgcc aggttgccgc actgagcgat
     2521 cgcgatttcg ggaagcgaac tcgaatgcag cgacccgacg caatcggaga gctcggacgc
     2581 tcgctcgacg cactgagcga gcggctgtcg accagcgaga tcaagaagca gggccgcaag
     2641 gaccgcttgc aaaccatagt cgacgcgatg gcggaggccg ttctggtaac cgacgcagaa
     2701 ggacggatca agctaagcaa cgaagtgctt gccgacctcg tgggcttcga tcccactggt
     2761 aagaccgcga tcgaagcgat tcgtcacccc gattttcatc gggcgattga agatgcgcaa
     2821 cagggctttt ccggattgca cgagatcgaa atcaacggac tgaatgctcg gagccggcat
     2881 atcctccggg catcggtgtc cccactgaga aatcagcagg gcgtcgtagc cgtctttcac
     2941 gacgtcacgg ctgaacgagc agcggctcag gttcggcgcg acttcgttgc caacgcaggc
     3001 catgagctcc gcacgccatt gaccgcaatt cgcggctttg ccgagactct tcgcgatggt
     3061 gcgctcgacg atccgaaggc cgccatgggc ttcatcgagg tcatcctcag gcatacacgg
     3121 cgcttgcagg ccttggtgga tgacctggcc gacttgagca gcttcgaggg caaagagctc
     3181 gagctggacc tagtaccagt caacgccggc gctgcgctct ccgaagtcgt ccgcggactg
     3241 gaagcgcagt cgaaggcaaa gggcttgaag gtcaacctca ccgggctcga cgatgcgcct
     3301 ctggtgttgg ccgaggagcg tgccctcgaa caggttctgg tcaacctggt cgacaacgcc
     3361 atcaagtaca cgcccgaacg gggagagatc cggatcgaca tcgcgcccga aggaagggat
     3421 gtgttcatcg aggtcgcgaa ctccggtcca gggatccccg cccggcacct ttcacgggtt
     3481 ttcgaacgat tctatcgagt cgacgcaggg cgctcccgtg agctcggcgg gacgggtctc
     3541 ggactctcga tcgtgaagca cttggtggcc aaaatgcgcg gcgaaatcaa cgttgatagc
     3601 agcgccggat ggacccggtt ccagctctgc ctgcccctcg cagatagccc caatagcgac
     3661 acaagtgtgt cacgaaactg acacataatg cgcgtagcta gggtgccatg acacccgcac
     3721 gattcatttg tcacgcgctc gtcgccctgg gtctcacctc gaccgtgctc gcacaagagg
     3781 acccaagctc gaccgagact gggcagaccc agcaggtcgt ggtcgagccg ccggctgccg
     3841 acgcgatggc cgatgtcgtg gagccgaccg gggatatgac gggcgatatg gcggaaccaa
     3901 cggaggacgt ggcagagcct ctgcaggctg agaccccaga agtgacggag gtggtcgacg
     3961 cgccgcctcc cgaagccgag acgacgcacg agggccgtcg ctcagcagag gagctccggc
     4021 ccaagcgcga agacgaaatg atgggcgacc acccggtggg ccccggcgga atcaaatttc
     4081 ggcctggcac gggcttggaa atcaatagtg aggacgatga tttccaactg cgcatccgcg
     4141 ttcgcgttca gttcctatac acactcgtca aaggaaactt gggaccgcgc gaagcggatg
     4201 agcctgagga tctgcgaaac gatttccgca ttcgccgagc acgcttcatc ttccaaggtc
     4261 acgcgttcgg gaagaacaac aagtacaagt tcgagctcga tcctctacgg cctgacaatg
     4321 tcgtcctcga ctattacctc gacttcacac aaaacaggaa catcgaggtt cgtgttgggc
     4381 aatacaagat ttcttcgaac cgtcagcgtg tgatctcctc gggcaacctg cagatggtcg
     4441 atcgatctgc tgtcaacgcg gagttcagcc tcgaccgcga catcggt
//
LOCUS       DASRZF010000403        31211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2368320, whole genome shotgun sequence.
ACCESSION   DASRZF010000403 DASRZF010000000
VERSION     DASRZF010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 31211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31211
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2368320"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..1046)
                     /locus_tag="VFG22_17820"
     CDS             complement(126..1046)
                     /locus_tag="VFG22_17820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET6418159.1"
                     /translation="MARPRIAFVGSGGAAKGIAHIGVLRAMQELDIEADICVGASAGA
                     IAGAFYSQGYTPSQMIDWFRPFWQRGLEAPPLKARYFLGLPNKEQRRSWGYLTSGLLS
                     IDRFERFVASQLPSNDFRDLEKHLLITAADIDGRGRVVFGRGYRDDVPISQALAASTC
                     VPGLFRPHRIGDRFYVDGEVVRTLSIDLAVEAGADVVIISNVYRPHVGRVGSPSLVYD
                     GALAVARQSLNIILNEKEKRGIDLIHKMYPQMQVLVVSPDLGRMPFTSRLHARTMLRR
                     GYRDALRILAKAKRRGVFDVAPNVRSIGKA"
     gene            complement(1106..2014)
                     /locus_tag="VFG22_17825"
     CDS             complement(1106..2014)
                     /locus_tag="VFG22_17825"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6418160.1"
                     /translation="MRDVDDEVRQIKKEIVESRGLIIKTNNLVNALGADVKSIAKRQA
                     GYERRLNWNSWVAIAVIGAVSFAGLKLYFDAQTGGMRSAMAEAETTLEELRQDLDDEV
                     QRASERASAAAKAAKYYDEIRARKRVEVVRDYPAIAKEALSPAEAAVFRDFEQRFRQD
                     LSIEAYQEGLGLAGAKKHEEAIKHFEEAIELHPNGSQIPAVKRSLALSLRSDKRSAEA
                     LVVAQQVAEQTTDSALQPDGWWLVALCARDVGDLDTSREALKLLIKKWPRSALSRDAR
                     PMLRDVTRESYQGKKALVAPAADAAE"
     gene            2109..2525
                     /locus_tag="VFG22_17830"
     CDS             2109..2525
                     /locus_tag="VFG22_17830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418161.1"
                     /translation="MGLRGIIFLASSTILVVAGCNQPADYALVGSAFVPSAQGEIEVE
                     NGDGGEILINMTIDQLVSPERIELGLTHYVVWFTSVGALPQRQGTLDYDPETQTGHAS
                     IPTKLREFELQITAEAGETPNRPSDLVVASQKIREK"
     gene            2530..3093
                     /gene="efp"
                     /locus_tag="VFG22_17835"
     CDS             2530..3093
                     /gene="efp"
                     /locus_tag="VFG22_17835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HET6418162.1"
                     /translation="MADTSDIKKGFKMLIDGVPFAVVDHQFVKPGKGQAFVRTRIKNM
                     MTGAVIDRTFKSGEKLEKADTEERTMQYLYPEGEGRVFMDTQSYEQLALSNEQLGDSV
                     HYLLDGTQVDVMLFQGRPIGVTPPTFVELLVTETEPGFKGDTSSNTTKPATLETGLQV
                     NVPLFVNAGDRLKIDTRTGSYVERVKG"
     gene            3102..4016
                     /gene="epmA"
                     /locus_tag="VFG22_17840"
     CDS             3102..4016
                     /gene="epmA"
                     /locus_tag="VFG22_17840"
                     /inference="COORDINATES: protein motif:HMM:NF006828.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HET6418163.1"
                     /translation="MAESADRALLQILELRAGAFSAIRRFFGEREFIEVDTPSVVPSP
                     GLDPHLAAFEVRAPKGKSVGWLATSPEYQMKRLLSAGAKRIFQLSRSYRADEQGQHHE
                     REFTMLEWYRADAGSEDVIRDTEALVAFVAESLDQPAPEVQPPWERMTVEDALQFHAN
                     VTLESLVDDEESFFRIWVEQVQPRLGGEQPVVVTDWPASMASLARIKDNGMADRFEAF
                     YRGVELCNGFGELTDPDEQRRRFERDQEQRRALGVAVYPIDERFLDALKSGIPESGGN
                     ALGVERLLMLLLGADSIQAVMPFPEERL"
     gene            complement(4021..4806)
                     /gene="mazG"
                     /locus_tag="VFG22_17845"
     CDS             complement(4021..4806)
                     /gene="mazG"
                     /locus_tag="VFG22_17845"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HET6418164.1"
                     /translation="MSNQGESLTRLVELMRTLLGPDGCPWDREQTLQSLRQYVVEEAH
                     EVVDAIDHGTPEMLREELGDLLLQVVFQASLTEQKGWFDIDGVVDAICEKLVRRHPWV
                     FGEEKVDDASGAINSWEAIKASEKKESGALSGVPLALPSLLRAVRIGEKASAVGYDWP
                     DAGGPRDKIDEELKELDEAAESGDMAALEHELGDLLFALATYGRKQGLDPEAALRASL
                     NRFSSRFRHCERAAEAAGKGLRDYDERELDAMWERAKAVERQA"
     gene            4869..5474
                     /locus_tag="VFG22_17850"
     CDS             4869..5474
                     /locus_tag="VFG22_17850"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6418165.1"
                     /translation="MAYAKSAVSSQQIIDAAIRVLARQGYAKTSLLEIAKEAGMSKGA
                     LHYHYPSKEALIHAVLEAACNAVQLRTLAAWSPSDNPFEALRKSLEELWAARAERSDE
                     AMVVADLLALSLYNEALRPRLAEFYELGAEQIRAYLQEHLISLGLEPRISLDILPRVV
                     IGLLDGLVMQAFVDPEALSPDDVVDAVQTIAIAIFSGGSGK"
     gene            5471..6454
                     /locus_tag="VFG22_17855"
     CDS             5471..6454
                     /locus_tag="VFG22_17855"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6418166.1"
                     /translation="MSPLESVKAQPGAVRALMRAREHGRVASAYLFDGPSGVGKELAA
                     LALATEIVAGGDRRIAERIRSDAHPDVRVFRPRDEGKRNIQVEVLRTEILPLAQFAPF
                     EASSTFFIFPEADISFPPFQPEAANALLKTLEEPRPNVHFILTSERPESLLTTIRSRC
                     QRIRFGRLPDAVLTQILESEGVNEASIASAVALSSGRADLALALARDGSVEALTALVI
                     SVDDAVRGTGPGTLVELSEKLVKRDDLELVLLTLQTFYRDLALSSLGVPAEALGFSSL
                     SEDLKERAAPITTSAAAARVSLIHQCVQSMRKNASPQVALDSLLFGLRNLR"
     gene            6494..8479
                     /gene="metG"
                     /locus_tag="VFG22_17860"
     CDS             6494..8479
                     /gene="metG"
                     /locus_tag="VFG22_17860"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HET6418167.1"
                     /translation="MARPFYVTTPIYYVNDRPHIGHAYSTVAADVVSRYQRLRGKPTY
                     FLTGLDEHGLKIERRAKEEGLAPQDFVDRMAAPFRLAWEELGCAHDGFIRTTSTQHRE
                     NVQALWELMQANGDIYLDDYEDWYCVGCESFKTEKELLEGNRCPIHQKPVERIKERSY
                     FFRLSRYTKPLLDHFEAHPDFVQPEARFNEVKSFVKEGLRDLSISRTSFRWGVPVPND
                     PEHVVYVWLDALMNYASALGGPAKEGEAPLYDEFWNASEAIIHIVGKDILRFHAIYWP
                     AFLMSAGIRLPTQIWAHGWLTVNGEKMSKSLGNFLPPGPLVEAFGADVLRYYFMREVG
                     FGQDGDFSHRNLLNRYNGELANGLGNLMNRIVASIVKKSFGGRVPKSDLRSLEEIDRQ
                     LIEKARSTSLEVAKHFEALAFHRALDAIWELVSAANKYVDSTEPWRLAKQDDQTRLEQ
                     VCYTVLETLRWLSIMLFPVMPEKCNELRAQLGMSPLFPTEQVDLWPSAWGGLRSGTQT
                     QTATPLFPRFDESQEHSILERLGVEAPRAEKKKASAMKENEPEKKPFLEFDDFMKVDL
                     RVALIKEAEKVEKSKKLVKLIVDAGDPEPRQILAGIAEHYAPEDLVGRRVVIVANLQP
                     RKLMGLESQGMVLAASDADGLSILGVDRDVAPGSSAK"
     gene            8476..9486
                     /locus_tag="VFG22_17865"
     CDS             8476..9486
                     /locus_tag="VFG22_17865"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET6418168.1"
                     /translation="MIPPLAPTFEAALRDVRAQDPRFRTASAAKLGEAPMERRDEAVQ
                     GLLHLANDEVGAVREAAYESLGALAAIEGLRTVLDAFDDPNLGARQSAVMAAGRIAPD
                     ESADAITALLHDPRPEMRYSAIWTLSHLGTEYSSRIRAGLADADQEIRALTAQCLSEL
                     GATESSNELADLLTDPADAVRFAAATALAALGDARGASVLRAALGEPDRALPAAIGLG
                     DLHDQDSVPALKRLAAHPWRSPILRAAAARALVRLGDPLGTEVIRKIVRSWRIEARQY
                     AVQLVGELGLISVLPDLRRALRRSRLSERPVYETTLEQLAPSSSEAKALLASLRQANQ
                     SR"
     gene            9497..10276
                     /locus_tag="VFG22_17870"
     CDS             9497..10276
                     /locus_tag="VFG22_17870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET6418169.1"
                     /translation="MLFDTHCHLDDPRLFDELGEVLERAEQAGVRRITSIGCASDVES
                     VMSAVRLAKQHPNRISATVGVHPHDAKHLDDALYDAIRDAGSDASVVAIGETGLDFHY
                     DHSPRQAQEEAFRKQVTIARELKKPLVIHTRAAPEQTLRILREEQAKDVGGIIHCFSE
                     DAPFAAAALDLGFVSSFSGIVTFKKAVAVQEAALKQPSDALLIETDAPYLAPIPKRGK
                     RNEPAYVAYTAARIAELRGEDPELLAQTTYQNALRIFGIAD"
     gene            10363..11088
                     /gene="rplC"
                     /locus_tag="VFG22_17875"
     CDS             10363..11088
                     /gene="rplC"
                     /locus_tag="VFG22_17875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HET6418170.1"
                     /translation="MNTNPGLIGTKLGTTQIFLDDGEVRRVTAVKAGPCVVVGKRTLS
                     KDGYAALQLGFGSRREKLVSKPEAGNFKKAGVDAQRVVREFRIDESLLERFEVGQTLG
                     VADIFQDGQFVDVSATSKGRGFTGVMKRHNFAGAGTVGHGTHEAKRHGGSIGQNMTPG
                     HTRRGQKMAGQYGNKRATLLNLKIVKVLSDENIVLIEGGVPGPRNGVITVKAAVKKIV
                     PPLPERAAKAEANVPPPEATEEA"
     gene            11089..11679
                     /locus_tag="VFG22_17880"
     CDS             11089..11679
                     /locus_tag="VFG22_17880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HET6418171.1"
                     /translation="MGPRRQQDHHGRRARREGYPARSVYKLQEIDRRVGLFKKGQRVL
                     DLGASPGSWTLYSAEQVGPTGRVLGIDLNDPDVALPPQAEIRTLDAFEVDPASLGGPF
                     DLVISDMAPKTSGQRHADQYRSYELVMRALELAKTVLKPGGTFVAKIFQGAEFDDARK
                     SLRDSFGKVRTMRPEATRDESYEVFLVGIDFKPASS"
     gene            complement(11669..12553)
                     /locus_tag="VFG22_17885"
     CDS             complement(11669..12553)
                     /locus_tag="VFG22_17885"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6418172.1"
                     /translation="MSRPPASPEVLRGERLFSEGKVGEAKRAFEQALADDPTDPRAWL
                     DLGLVHEAKGDFGSAEAAYRRATELDQNFAEAYNNSGVLLRETGKLAEATTALERSVA
                     LDPDLTPARFNLALCYEELGKMADAEREYLATIEDLPNDPVPRINLAMMLIDLRRFDD
                     AAAQLRLVHPMVRGDVLLSIAVGEGFRRAGLPDDAVSVLRPALEQAPEPPPTELLGEL
                     SLAYYASGDLGSAEATMRRAVAQDELDPGLQYAYGSILAKQGQVGKARVHLRRAVSLD
                     PGGPYAERARARLESLKN"
     gene            complement(12649..15339)
                     /gene="glnD"
                     /locus_tag="VFG22_17890"
     CDS             complement(12649..15339)
                     /gene="glnD"
                     /locus_tag="VFG22_17890"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HET6418173.1"
                     /translation="MSDGSSPVVDLGGLAPTLGKTCSGYLGGYRRAFEDAVRRGDPGV
                     STARAHARALDGLLGALYCAADAATRAQGCEPGGRVALVAVGGYGRRTLGLHSDVDVV
                     FLCDDSDDPFVAALAEGLLYPLWDLGVDIGHAVRSVGETLRLAREDIRTTTTLIDFRR
                     IAGSGSLLDDLERGAREQVFEPYLVEFLGALEQDTETRHRRFGGSLYLLEPDVKQGRG
                     GLRDMDVAEWAARARWGARNEQDYVRTGALLAREVRELDAAREMLWRVRNLLHLRAGR
                     QQDRLTFGDQEDIAQELGFVDGVTLGVEQFMQAYYRHARIVALTAERMLDRARPRNRA
                     RATTFRKLADGIVLADGTISLEKPQRLDADPALAFRFYRQALRYQKRPDHSALDAIAR
                     IAPDKRWRLRLQGSEEATQLFLALLKDVDESPFRSGSTIAELHEVGLVSAMIPEFEPL
                     VGRVYHDVFHVYTADIHAVKALEYLQALTRGESQGRALSARLAAEAPRRLSLFLAVLL
                     HSLGRARGRDQDEAGARLAESVALRLGLSAVDAQHVAWLVREHKSLYRWATQRDIHDP
                     RGLTEVVKIVETPERLRDLFLCTVSVIATVNPKAMTSWKARALEDLYLATLAQIESGS
                     APAHTEGRVLEVKLQAIVGFAGDAGEEELQSFIDEMPDRYFLANPVDAVRRHARISRD
                     RSSGIATVRVGPGPSEDVEEVVVISADRPGLLADFTAVFAAHDLSILAAQLYTRRRKS
                     GAEAFDLFWIKVGDNTPTPTRLAEALQEDIDHRLTNRVSAQDFIARQSTPPPWSVRPG
                     PDVPTHISVDNNASSQYTIVDVFTRDRIGLLHEIARTLHQLDLTIALSKVNTEGQSVA
                     DVFYVSDEDGNKVHDPERLKKLQRVLYDRILGLHARAEVP"
     gene            complement(15387..16532)
                     /locus_tag="VFG22_17895"
     CDS             complement(15387..16532)
                     /locus_tag="VFG22_17895"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HET6418174.1"
                     /translation="MLGLLSLSLSCRSDSPPSPDRDLPRPLPELPEAVEPAREKARLV
                     RIDVYGVDSKDAAGARVVLLFEGAPLFQQKQLPPQGVMPARISIELQDAEWGAELPVS
                     QAVERAGLKRVRLATPNPRVVLDLLPGATGRVFYLTDPYRIVVDVSADRRPRRSDRPL
                     VVLDPGHGGPEPGAANDRYGLVESQIALELAELTANRLREIMPRARVMLTRTGDVDLS
                     LEERCAMANSLEADAFVSIHLNASSEQVRRGGVTTFVLDTTNNRQALRLAARENGTRV
                     AEVTGIQSLLAKHHRQTQAEGSLTLADKIQRHTLKRGRSLLPELSDRGVRRAMFYVLV
                     GARMPAVLVEASFLTYEPEARALAKRSYRRALADGVASGIVSYLQAH"
     gene            16652..17677
                     /gene="purM"
                     /locus_tag="VFG22_17900"
     CDS             16652..17677
                     /gene="purM"
                     /locus_tag="VFG22_17900"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HET6418175.1"
                     /translation="MAGLTYRDAGVDIEAGEQLVERIKPLAQSTRSEHVLGSIGGFAG
                     LCSLPSDIEDPVLVSGTDGVGTKLKLAFELERHDTIGIDLVAMSVNDIITVGARPLFF
                     LDYFATSKLDVDQGEAVIKGIAEGCRQAECALLGGETAELPGFYATGEYDLAGFAVGV
                     VSRKRIIDGESVRLGDQVVGVASSGLHSNGFSLARRVLFGGDTPLSVHDSLDSGIALG
                     EALLRPTRIYVKAARLALDHDVHAMCHITGGGLPENLPRVTPKGLGIEIDPSSWPIDP
                     IFTLIQERGSVADAEMRRTFNMGVGFVMVVNPGRAKGLVDALSAAGEKAFVMGEVVDR
                     EGVSFSG"
     gene            17686..18324
                     /gene="purN"
                     /locus_tag="VFG22_17905"
     CDS             17686..18324
                     /gene="purN"
                     /locus_tag="VFG22_17905"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HET6418176.1"
                     /translation="MDVVVLVSGRGSNLRAICTAIDAGACDAKIVGVVSDRKSAAALE
                     FAQARGVPTKVVPLRKSDDRDRWNELLAQEVAQLDPELIVLAGFMRVLGRPLLDRFPG
                     RIINVHPALLPSFPGHDGPQDALAGGVRVSGCTVHIVDAGVDTGPIIAQAAVPVLVDD
                     TAETLHARIQVQEHRLLPGVIHQIAIGAITLAPLTVRTAVADPTQSLTVPDA"
     gene            18329..19852
                     /locus_tag="VFG22_17910"
     CDS             18329..19852
                     /locus_tag="VFG22_17910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418177.1"
                     /translation="MPTSFYDVVVLGTNLEPLLCAALLAREGLRVLVLGQDVPEPSYT
                     LAGVEVEPYAMVVAGAQSPAVANTLDELALRQDLRQRIVERPSVLQLLLPEHRLNVHM
                     ENEKWLAELDRELPDVRGQAVDITRTLREVRTEIDTMVSRRLIWPPETFIERQRFSLA
                     ATTQRYDRLGLGWTSWNQLPKRHPLRTAFEAMLPHVSGLVPTQHSDATRARLHGHLLY
                     GATELSGGWPWFREALFARIRSWGSDVRPRDSARSIQLHGSRGHTIRLARSDEEIGCS
                     QVAHGTSISELSQLLPDRSSMTAMFERVGEPRSRAYRCTVHVLIDARGVPKALEPLAL
                     LCASATDTGKAFLLRTHEVDDERMLISTSGLIDEHRIDSTSSRLQFVREEALDAIRSV
                     IPFLDEHAIWVDSPHDGLPPRSLRGEGELSCSDPWVRGPSTMKSVYEFPTRRALGVCA
                     LPTRTPVRGVFLCNDQIAPGLGFEGSFLAATSVARLIGSEYRRQDWLRRGPWARRSV"
     gene            19888..21015
                     /locus_tag="VFG22_17915"
     CDS             19888..21015
                     /locus_tag="VFG22_17915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HET6418178.1"
                     /translation="MAAPEEAPRLTLRQLLRLMVERGSSDLHITTGSPPQLRIDGSLV
                     PLRTAPLKPRESKALCYEVMTEDQRLRFEKGHELDFSFGVKSLSRFRANVFLQRGAVS
                     GAFRAIPFNIRPLSELGVPPVVEELCTRPRGLVLVTGPTGSGKSTTLASMIDKINSEH
                     RHHIVTVEDPIEFLHSNKLCVVNQREVGSDTAGFKDALKYVLRQDPDVVLVGEMRDLE
                     TIEAALTISETGHLVFATLHTNTAVQSINRIIDVFPSGQQSQIRAQLSFVLQGVLSQM
                     LLPRADGPGRAMACEALVPNAAIRNLIREDKIHQMYGIMQTGQGSSGMQTMNQSLLHL
                     VAHGALDRELAEIRSPEPDEFRQMLVHLDQKGYHQAIRAGG"
     gene            21286..22041
                     /locus_tag="VFG22_17920"
     CDS             21286..22041
                     /locus_tag="VFG22_17920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418179.1"
                     /translation="MPTSTALLQAIRAHDKAGNSIRAANLALQLRDEYPNDERSREVA
                     KSVIAATKLDSVLVESHCEKECTLELDGALIQHPTFFVTPDVEHSLKAAFDNGETSTY
                     VQGAAGDVKNVTIAAPAPPPPPPVPRWAFFSSLGATVALGAVTVWSGIDANNGVSAYE
                     SAARTANSPGINGSGSPTPEQQAQSLLDEGQQKERRTNILIGVTAGMAASTIVLGVFT
                     NWKGKPPETTAKRLEPSIGVGAKGGSIAVKGRF"
     gene            22089..23651
                     /locus_tag="VFG22_17925"
     CDS             22089..23651
                     /locus_tag="VFG22_17925"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6418180.1"
                     /translation="MAGRQLGRYEILTQLASGGMASVYIARAQGVAGFERLVAIKVLH
                     PHLAYEQEFISMFLDEARLAARIRHMNVVPTLDMSDSPGDGYFLVMEYIEGDHLGALL
                     GCAAKQGERLPKPFVCRVLVDTLQGLAAAHRLTDENGNPLKLVHRDVSPHNILVGTDG
                     IARLTDFGVAKADVRMASTRAGQFKGKLSYMAPEQASSSETDQRSDLFSVGIILWECL
                     TGRRLFKGESNAATLNKLLNDAIVKPSVLWPDLEPFDAVVMKALNRDPERRFQSADEL
                     GEAIDLASGRNGIAKTRDVAQVVRTLDAEKLQEERQRVRDAIEMLGRAEFAESTIPMP
                     REGIPAKGTSIRHGSATGSYIHGSVSGVLEDGTPVVVRPAGDSNALKWFVMALAVLVF
                     LYAGFLVWELLLTPSARRAPEPSRAVEVAAPVPVAPEEPRATKRAEPGVEVAPDPTRE
                     TEQIDGVPERKSSAEPSRPARRRARAKSRANVPRAAESKKAENREAESPPSVPASKAD
                     PVDIDVRNPYRN"
     gene            23756..24967
                     /locus_tag="VFG22_17930"
     CDS             23756..24967
                     /locus_tag="VFG22_17930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET6418181.1"
                     /translation="MAEWVYEARTRAGEVQTGIIEADSKEAVQSRLRARQLNPVKIKK
                     KGRELKLSFGTGVTPKELVVFTRQFATMIDAGLPLVQCLDILSSRGENKALNAILKDV
                     KDHVEQGGTFSEGLARHPKLFDELFVNLIRAGEMGGILDTILNRLAGYIEKRVKLARQ
                     VRGAMVYPIAIFCVAIIVVVVMLAWVIPSFKGMFAEFGGEDSLPALTQIVISASEGFM
                     SNVHWIILATVGFAFGVSWVYRQPAGRHFFHRTLLVLPILGPVMRKISVARFTRTLGT
                     LLSAGVPILDSLDVVKKSAGNVVVENAIQQTSDKIREGRTMAEPLMETNVFPPMVVQM
                     IGVGEQTGALDTMLNKIADFYEDEVDIAVAALTSLLEPLMMVFIGGIVGVILISMYLP
                     IFSIAGKVNAD"
     gene            24964..26679
                     /locus_tag="VFG22_17935"
     CDS             24964..26679
                     /locus_tag="VFG22_17935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6418182.1"
                     /translation="MNQTQERLPQDHEEPNPHRRLQWVLGGRLIIATVLLGATMYLAL
                     DAIRYGRFTPTFLLILISAIYASSIVFGVWLLTGRQENRVAVAITTLDVLLITGLVYL
                     TGGAGSIFSFLYGAQILTAALTLGTNAAYYTAAASLACYFLQGVSLNLGWLPPPPDQP
                     LSQYVLSSRDFQLALVSNMVGIAAVALLATNLARRAQVAGARLIEAEESAARLVRLND
                     DIVRSIASGLITAGDDGLILGINRAARDILRDREGSLLGQSLSAVLPNYALERLSEPP
                     RRADAIGRRADGGEFPMGYTLSALFDAEGNRSGLLLAFQDLTEIRTLRNRAERAQRFA
                     ALGQLATGLAHEIRNPLSAISGSVEMVREGNALGPEDRRLLGIVISEVERLNSLVTTM
                     LQVGRPGQIEAEPHDLRSVASEVVAIARGEATASNGLEIEAVDPGIPIIVSLDPDRMR
                     QVVWNLVRNAVQASPRRGRVEVRTGRDPSGCAFLEVADEGPGIGADHRERLFDVFYSG
                     RTHGVGLGLALVKQIVDQHEGRIDIVDREGPGTCFRVTLPRGESSLRPNAAAQPDEHP
                     DPPSA"
     gene            complement(26624..27421)
                     /locus_tag="VFG22_17940"
     CDS             complement(26624..27421)
                     /locus_tag="VFG22_17940"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase"
                     /protein_id="HET6418183.1"
                     /translation="MTDYLSAILARTHRENLRRRRHRSRMRPVSRIAQPERSERGVRA
                     LRRMPGGAPSVIAEVKFRSPSAGEIRAQKPGEGVRVAEGYERGGAAVVSVLADGPGFG
                     GSPLLVRRVAQAVSVPVLFKGFVLDPIQVDLAYDVGASLVLLLVRALEDSDLRMLMAR
                     IRELGMEPVVEAANGEEVDRAVAAGSTIIGVNARDLGTFSVDKAAAQLCIERIPEGRI
                     AVFMSGVQSVEDFREVAKGRADAVLIGEGLMRSADPGARLAALLRSV"
     gene            complement(27418..28434)
                     /gene="trpD"
                     /locus_tag="VFG22_17945"
     CDS             complement(27418..28434)
                     /gene="trpD"
                     /locus_tag="VFG22_17945"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HET6418184.1"
                     /translation="MISTAIQALVSGRDLSADEVEAAMDVILDGKASGAQIAAFVVAL
                     RMKGESAVEIAAAALALRKHCQTIQPQVDGPLLDTCGTGGDGLHTFNISTAAALVAAA
                     SGIAVAKHGNRAVSSKAGSADVLEALGVRIDLPPERVERCIEEVGIGFLFAPSHHSAM
                     RHAAPVRRELGIRTMFNLLGPLSNPASATHQVVGVYDASRVEQLAQALGALGLTGAWV
                     VHGAGGLDEVSPAGPTKVAQLAEGEVTTFEISPRDFGLPEVPIEALRGGDAARNAEII
                     RAVLRGDEGPARVAVLLNAAAALRVAGLARDPVDAARRAAEAIDSGAAAVTLERWAQL
                     TQSP"
     gene            complement(28431..29015)
                     /locus_tag="VFG22_17950"
     CDS             complement(28431..29015)
                     /locus_tag="VFG22_17950"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HET6418185.1"
                     /translation="MILVIDNYDSFTYNLVQYLSELGADVAVERNDAVSVEEIRERRP
                     EGILISPGPGRPEEAGVSLDAIAELGADIPIFGVCLGHQSIAQHFGASVVRAERLMHG
                     RTSQIHHEGRGVFKDLPSPLTATRYHSLIVKRDTIPSTLEVTAWTDQDEVMGIRHVEL
                     PIEGVQFHPESFLTEHGHDLIRNWLSSVPASGKS"
     gene            complement(29050..29946)
                     /locus_tag="VFG22_17955"
     CDS             complement(29050..29946)
                     /locus_tag="VFG22_17955"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6418186.1"
                     /translation="MMPSPVEERRFVSFDGTEIAYQSVGQGRPILLINGLGGGWEAWS
                     HQIQYFRDRYRLLSWDYRGLYDSSRPADPATLAIADHARDALCLLNEVGAERAALVGW
                     SMGVQVALEVFRAEPERVASLAMVNGVAGRPWDYVFNLNLVGRLLPPFLRGLRSMPRT
                     IETVVHQAMRVPDPGGWIKRMGLAASTLDVQLVAALAEKFRTLDMDTFLRLLEKLGEH
                     DGWDLLPLIDVPMLVIAGTRDVFTPRSAAERMVRRTRGSEIMLIPGATHYAALEYPEM
                     VNLRLEKFFRERGYEPSPAGAS"
     gene            30034..31149
                     /locus_tag="VFG22_17960"
     CDS             30034..31149
                     /locus_tag="VFG22_17960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418187.1"
                     /translation="MSDELQKSKKVRDIRERLGRNTAAPPPQSLEEGGVAPPPPTLGG
                     VAPPPGFVGGLAAKRSGPFGATAAKAPAQQEVRIVVDEQAVATAHEGKSRRVRKLMMM
                     TGGGAALLGLFIGMLASTVMGKRSEYNIAVRDGKAVYEGVRAAADQVTEAKRLLDRAM
                     ASAKARPGEGPSVDYEAIEQLRALPTPFTAADFTGLNYTKFNRETVNSLFVYARQVGE
                     LWDQFDGLAGRVLPESRRAELDEAAQDETAITTSPTGCVPTLGEGGFRCGLVYVDMPE
                     NAAATTLKVRATRGSKAFEKELYMGQELRQNASNYVILTNPEQSAGVLGQRKNAFASY
                     RRDLAELGRLMETTLETQGTLEKGLGSVAGLEEIVSF"
BASE COUNT         5989 a         9915 c         9641 g         5666 t
ORIGIN      
        1 atcgcgagca cgcccgtcgc gagccgtcct ccggtcaccc agccgcgcaa cacagttgct
       61 ggcgccaaac gaacggggca cgggcgaacg cggcgcgtcc cggtgatccc gagagccgcc
      121 ggcgtttacg ccttgccgat gctgcgcacg ttcggcgcca cgtcgaagac cccgcgacgc
      181 tttgcctttg cgagaatgcg aagtgcgtcg cggtatccgc gtcgcagcat cgttcttgcg
      241 tgcagacggg acgtgaatgg catccggccg aggtccgggg aaaccaccag aacctgcatc
      301 tgcgggtaca tcttgtgaat caagtcgatg ccgcgctttt ctttttcgtt gagaatgatg
      361 ttcaacgatt ggcgcgcgac tgcgagagcg ccgtcgtaaa ccagagacgg gctccccact
      421 cggccgacgt gcggacgata gacgttggag atgatgacga cgtcggcgcc ggcttcgacc
      481 gcgagatcga tgctcaaagt tcgaacgacc tcaccatcca cgtagaatcg atcgccgatg
      541 cgatgtgggc ggaagagccc tgggacacag gtcgaggcgg ccagggcctg actgatcggc
      601 acgtcgtcac ggtacccgcg accaaagacc actcggccgc gaccgtcgat gtccgcagcc
      661 gttatcagca gatgcttctc gaggtctcga aaatcgttgc tcggaagctg gctcgctacg
      721 aagcgctcaa agcgatcgat cgaaaggaga ccgctggtca agtagcccca gctgcgcctc
      781 tgctccttgt tcgggagacc gaggaagtac ctggccttca gaggcggcgc ctcgaggcca
      841 cgctgccaga acggacgaaa ccaatcgatc atctgcgatg gcgtgtatcc ctgagaatag
      901 aacgcaccgg caatcgcacc cgcagaagct cccacgcaga tgtcggcttc tatgtcgagc
      961 tcctgcattg ctcggagcac gccgatgtga gctatgccct tcgcggcacc accggaacct
     1021 acgaatgcaa tacgcggccg ggccatcgat agacaacttg gcacacgtta gcgaccgagt
     1081 cgacacgcaa ttcctgcgcg gcgcttcact cggcagcgtc ggccgcaggc gcgaccagcg
     1141 ccttcttgcc ctgatacgat tcccgcgtca cgtcgcgaag catcggtcgg gcgtcgcgag
     1201 acaaagccga tctcggccac ttcttgatca gcagcttgag cgcttcacgg gacgtatcga
     1261 gatcgccgac gtcgcgcgcg caaagggcca cgagccacca gccatcgggt tgcagggcgc
     1321 tgtcggtggt ttgctcggcc acttgctgcg cgaccacgag cgcctccgcc gaacgcttgt
     1381 cgcttcggag agacaaggcc aggctccgct tgaccgcggg gatctgtgag ccgttcggat
     1441 gcagttcgat cgcctcttcg aaatgcttga tcgcttcctc gtgcttcttc gccccagcga
     1501 gccccaggcc ttcttgatac gcttcgatgg agaggtcctg gcggaaacgc tgctcgaagt
     1561 cgcgaaacac ggcggcctcc gccggcgaca gggcctcttt ggcgatggcc gggtaatcgc
     1621 ggaccacctc gacgcgcttc cgcgcgcgga tctcgtcgta gtacttggcg gccttggcgg
     1681 cagccgacgc ccgttcagat gctctctgca cctcatcatc caggtcctga cgcagttctt
     1741 cgagcgtggt ctcggcctct gccatggcag accgcattcc cccggtctgt gcatcgaagt
     1801 agagcttcag ccccgcgaaa gaaacggcgc cgatgacggc aattgcaacc cagctattcc
     1861 agttcagccg tcgctcgtat cccgcctgac gcttggcgat cgatttaacg tcggcgccga
     1921 gggcgttgac caggttgttg gtcttgatga tgaggccgcg gctttcgacg atctccttct
     1981 tgatctggcg cacttcgtcg tcgacatctc gcatgaagcc cggagtacaa ccgacccgga
     2041 cgcgaggcaa gggcaggcgc ttgacgggac cagatggtcc gcctacaagc cgtgccgagg
     2101 agcatttcgt ggggttacgc ggtattatct tcctggcatc gagcacgatc ctcgtggtcg
     2161 cgggttgcaa tcagccagct gactacgcac tggtcggcag cgcgttcgtg ccgtcggcgc
     2221 aaggggagat cgaggtcgag aacggcgacg gtggggaaat cctgatcaac atgaccatcg
     2281 accagctcgt ctcgcccgag aggatcgagc ttgggctaac ccactacgtg gtctggttca
     2341 cctctgtcgg cgcactaccg cagcgccagg ggacactcga ctacgacccc gaaacgcaaa
     2401 ccggccatgc ttcgattccc accaagttgc gggagttcga gctgcagatc accgcagaag
     2461 ccggcgagac cccgaatcga ccgagcgacc tcgtcgtcgc gtcccagaaa atccgcgaga
     2521 agtaatccca tggcagatac ctccgacatc aaaaaaggct tcaagatgct catcgacggc
     2581 gtgcctttcg cagtcgtcga ccaccagttc gtgaagcctg gcaagggaca agccttcgtt
     2641 cggacgcgca tcaagaacat gatgaccgga gcggtcatcg atcggacgtt caagagcggt
     2701 gaaaagctcg agaaggccga caccgaagaa cgcacgatgc agtacctcta cccagagggt
     2761 gaaggacgcg tcttcatgga cacgcagagc tacgagcaac tcgcgctgtc gaacgaacag
     2821 ctcggtgata gcgtgcacta cctcctcgat ggcactcaag tcgacgtgat gctctttcag
     2881 ggccgtccaa taggcgtgac gcctccgacg ttcgtcgagc tcctcgtcac cgaaacggaa
     2941 cccgggttca aaggcgacac gagcagcaat acgactaaac cggcgaccct agaaaccggt
     3001 ctgcaggtca acgtgccact tttcgtaaac gccggagaca ggctcaagat agacacgcgt
     3061 accggctcct acgtcgaacg cgtcaaaggc tgagcacgcg agtggcggaa tccgccgatc
     3121 gcgccctgct gcagattctg gagctccgcg cgggggcctt cagtgcaatt cgccgcttct
     3181 ttggagagcg cgaattcatc gaggtggata cgccctccgt cgtgccctcc ccgggactcg
     3241 acccgcactt ggcggcgttc gaggtgcggg cgcccaaagg caagtccgtt ggctggctcg
     3301 cgaccagtcc cgagtaccag atgaagcgcc tcctgagcgc aggcgccaaa cgcatcttcc
     3361 aactcagtcg gagctaccga gccgacgagc aagggcagca ccacgagcgc gaattcacga
     3421 tgctcgagtg gtaccgggcc gatgccggct ccgaggacgt gattcgcgac acggaagcgc
     3481 tcgtcgcctt cgtcgcggaa tcgctggacc agccagcgcc cgaggtgcag ccaccctggg
     3541 agcgaatgac cgtcgaagat gcgctgcagt tccacgcgaa cgtgacgctc gagtccctgg
     3601 tggacgacga agagagtttc tttcggatct gggtcgagca ggtgcagcct cgactcggcg
     3661 gcgagcaacc ggtggtggtc acggattggc cggcgtcgat ggcgtctttg gcaaggatca
     3721 aggacaacgg gatggcggat cggttcgaag cgttttatcg cggagtggag ctctgcaatg
     3781 gctttgggga gctcacggac cccgacgagc agcggcgtcg tttcgaacgc gaccaggaac
     3841 agcgccgggc cctcggagtg gcggtctatc ccatcgatga gcggttcctc gacgctctaa
     3901 aatccgggat acccgaaagc ggtggaaacg ccctcggcgt cgagcgactg ctgatgctct
     3961 tgcttggcgc ggactcgatt caagccgtga tgccgttccc agaagagcgc ctctagcttc
     4021 ctacgcttgc ctctccaccg ctttggcgcg ctcccacatc gcgtctagct cgcgctcgtc
     4081 gtagtcgcgc aagcccttac ccgcggcctc agcggcacgc tcgcagtgtc ggaatcgtga
     4141 agagaaccga ttgagagatg cacgaagcgc ggcctcggga tccaggcctt gtttgcgtcc
     4201 ataggtggcg agggcgaaca agagatcgcc caactcgtgc tcgagtgcgg ccatgtcccc
     4261 gctctcggcc gcctcgtcca actccttcag ctcttcgtcg atcttgtcgc gggggccgcc
     4321 ggcgtcgggc caatcgtagc cgacagcgct cgccttttcc ccgatgcgga cggcgcgcaa
     4381 taggctcggc aaagccaagg gaactccact gagagctccg ctctctttct tctcactcgc
     4441 cttgatagcc tcccagctgt tgatcgctcc gctcgcgtcg tcgacctttt cttcgccgaa
     4501 gacccaaggg tgtcgacgaa cgagcttctc gcagatcgcg tcaaccacac cgtcgatgtc
     4561 gaaccaacct ttttgctccg tgagtgaagc ttgaaagacg acctgcaaga gaaggtcccc
     4621 gagctcttcg cgcagcatct cgggcgtacc gtgatcgatc gcgtcgacga cttcgtgggc
     4681 ctcttcgacg acgtactgcc gcaggctttg cagcgtctgc tctcgatccc aggggcagcc
     4741 atcggggcct agcaacgtgc gcatcagctc gacgaggcgc gttaaggact caccttggtt
     4801 gctcatcggc tgtgctctag cacaagcggt tgacgagccc caggccgagg cgtcatggtc
     4861 tcgcattcat ggcgtacgca aagagcgcgg tcagctcaca gcagatcatc gacgcggcaa
     4921 ttcgagtcct ggcccgccaa ggctacgcca aaaccagcct gctcgagatc gccaaggagg
     4981 cgggcatgag caagggtgcc ctccactacc actacccgag caaagaggcg ctgattcacg
     5041 ctgttctcga ggcagcgtgc aacgccgtgc agctgcggac tctcgccgcc tggtcgccat
     5101 cggacaatcc gttcgaggcg ctgcgtaagt ccctcgagga gctttgggcc gcccgggcgg
     5161 agcgcagcga cgaagccatg gtcgttgccg atctcctcgc gttgagcctc tacaacgagg
     5221 cgctccgtcc gaggctcgcc gagttttacg agctgggggc cgaacagatt cgcgcatacc
     5281 tacaagagca cctgatctcg ctcggactcg agccacgaat ctcactcgac attctgccgc
     5341 gcgtcgtcat cgggctgctc gatggactcg tcatgcaggc attcgtcgac ccggaggctc
     5401 tgtctccgga cgacgtcgtg gatgcggtgc agaccattgc gatcgcgatc ttctcggggg
     5461 gctcggggaa gtgagcccgc tcgagtcggt gaaagcacag ccgggggcgg ttcgtgcgct
     5521 catgcgggcg cgggagcatg gccgggtggc ctccgcgtat ctcttcgacg gaccgagcgg
     5581 cgtcggaaag gagcttgccg ccctggcact tgcgacggag atcgtggccg gaggtgatcg
     5641 ccgcattgcg gaacgaatcc ggtcagacgc acatccggac gtgcgcgtat tccggccgcg
     5701 cgacgaaggc aagcgcaaca tccaggtcga ggtgctccgc acggagatcc tgccccttgc
     5761 tcagttcgct cccttcgaag cgtcttcgac cttcttcatc ttccccgaag cggacatttc
     5821 atttccgccc tttcaacccg aagcggcgaa cgccctgctc aagacgttgg aggagccacg
     5881 ccccaatgtg cacttcattc tgacgtcgga gcgcccagag tcacttctca cgaccattag
     5941 gtcgaggtgc cagcgaattc gctttggacg acttccagat gcggtcctga cgcagatcct
     6001 cgagtccgaa ggcgtgaacg aggcaagcat cgcttccgcg gtcgcactga gctcgggtcg
     6061 cgctgacctc gcgctcgcgc tagcccgcga tggctcggtg gaagccctca cggcgttggt
     6121 gatctcggtc gatgacgccg tccgcgggac gggtcccggg accctcgtcg agctttcgga
     6181 gaaactggtg aagcgagacg acttggagct cgtgctcctc acgcttcaga cgttctaccg
     6241 ggacctcgct ctgagctcgc tcggcgttcc tgccgaggcg ctcggcttct ccagcctctc
     6301 cgaagatctg aaagagcggg cggcacccat caccaccagc gctgcggcag cccgggtctc
     6361 cctcattcac cagtgcgtgc aatcgatgcg aaagaacgcc agtccccagg tcgctctgga
     6421 ctcgctgctg tttggtctcc ggaatctgcg ctagctggcg cgtcggccga tattggctag
     6481 tctcgggcgc gcaatggctc gccccttcta cgtcaccacg ccgatctact atgtcaacga
     6541 tcggccccac atcggccacg cgtactccac tgtggccgcg gacgtggtgt ctcgatatca
     6601 gcgactccgc ggcaagccga cgtacttcct gaccggactc gacgagcacg gcctgaagat
     6661 cgaacgccga gcaaaggaag agggactggc accacaggat ttcgtcgatc ggatggccgc
     6721 gccgttccga ttggcctggg aagagctcgg gtgcgctcat gatggcttca ttcgcacgac
     6781 ttccactcag caccgcgaga acgtgcaagc gctctgggag ctcatgcagg ccaacggtga
     6841 catctacctg gacgactatg aagattggta ttgtgtcggc tgtgagtcgt tcaagaccga
     6901 gaaggagctt ctcgagggaa atcgctgccc gattcaccag aaacccgtcg agcgcatcaa
     6961 ggagcgcagc tacttctttc gcctgagccg gtataccaaa ccgcttctcg accatttcga
     7021 ggcgcatccg gatttcgttc agcccgaagc ccgcttcaac gaggtgaagt ctttcgtcaa
     7081 ggaaggcctg cgagatctct cgatatcccg gaccagcttt cgctggggcg ttcccgtgcc
     7141 gaatgatccg gagcatgtcg tctacgtatg gctcgatgca ctgatgaact acgcgagcgc
     7201 gcttggcgga cctgccaaag aaggcgaagc accgctctac gacgagttct ggaacgcgtc
     7261 agaggcgatc attcacatcg tcggtaaaga catcctgcgg ttccacgcga tctactggcc
     7321 ggctttcctg atgagtgctg gaattcgact tccaacgcag atctgggccc atggctggct
     7381 caccgtgaac ggcgaaaaaa tgtccaaatc gctgggcaat tttcttccgc cgggtcctct
     7441 ggtagaggcc ttcggcgccg acgtgctgcg gtactacttc atgcgcgagg tggggttcgg
     7501 ccaggacggt gatttcagtc accgcaacct actcaaccga tacaacggcg agctcgccaa
     7561 cgggctgggc aacctgatga accgaatcgt cgcaagcatc gtgaagaaga gctttggcgg
     7621 tcgggttccg aagtccgatc ttcgttcatt ggaagagatc gatcggcaat tgatcgagaa
     7681 agcgagatcc acttcgctcg aagtggcaaa gcacttcgag gcgctcgcgt tccaccgcgc
     7741 cctcgatgcc atttgggagc tcgtctccgc cgcgaacaag tacgtcgatt cgaccgagcc
     7801 gtggaggctc gccaagcaag acgaccagac ccgactcgag caggtctgtt acacggtgct
     7861 cgaaacgctt cgctggcttt cgatcatgct gtttcccgtg atgcccgaga agtgcaacga
     7921 gcttcgcgcg cagctcggca tgtctccgct gttcccgacc gagcaggtcg atctctggcc
     7981 gagcgcctgg ggaggtctcc ggtccggcac ccaaacgcaa actgcaactc ccctgtttcc
     8041 gcgattcgac gagagtcagg agcattccat tttggagcgt ctaggagtcg aggctcctcg
     8101 cgcggaaaag aagaaggcct ccgccatgaa agaaaacgag ccagagaaga agccgttcct
     8161 cgagttcgac gatttcatga aggtcgacct gcgagtggcc ttgatcaaag aggctgaaaa
     8221 ggtcgaaaag agcaagaagc tcgtcaagct gatcgtcgac gcgggcgacc cggagccgcg
     8281 ccagatcctg gcaggaatcg ccgagcacta cgctcccgag gatctcgtgg gtcgccgcgt
     8341 cgtaatcgtc gccaatttgc agcctcggaa gctcatgggc ttggagtcgc aggggatggt
     8401 gctggccgcg agtgacgcgg atgggctgag catcctcgga gtcgatagag acgtcgcacc
     8461 cggatcctcg gcgaagtgat tcccccgctc gctcctacgt tcgaagccgc cctgcgcgac
     8521 gtgcgagctc aggacccgcg ctttcgaacc gcgtcggcag cgaaactcgg cgaggcgccc
     8581 atggagcggc gcgacgaagc cgttcagggc ttgttgcatc tagcgaatga cgaggtgggt
     8641 gccgtgcgcg aggcggctta cgagtcgctc ggcgccctgg ccgcgatcga aggactcagg
     8701 acggtgctcg atgccttcga cgacccaaac ctgggcgcac gccaatccgc cgtcatggca
     8761 gccggccgga tcgcgcccga cgagagcgcc gatgcgatca ccgccctgct ccacgatcca
     8821 cgcccagaga tgcgttactc ggcgatctgg accttgtcgc acctcggcac cgagtactcg
     8881 agccgaatca gggcgggtct cgccgacgca gaccaagaaa tccgagctct gacagcgcag
     8941 tgcctgagcg agcttggagc cacggagagc tcgaacgagc ttgcagatct tctcaccgat
     9001 cctgcggatg ccgttcggtt cgcggctgca acggcgctcg cggccttggg agatgcgcgt
     9061 ggtgcgtccg tgttgcgcgc tgccctcggt gagcccgacc gagcccttcc ggctgcgatc
     9121 ggtctcggcg acttgcacga ccaggactcc gtccctgcat tgaagcgtct cgccgcacat
     9181 ccctggcgct cccccatctt gcgtgcggcc gccgcccgcg cactcgttcg gctgggcgac
     9241 cccctaggaa ccgaggtgat tcgcaaaatc gtgcggagct ggcgcatcga ggccaggcag
     9301 tatgcggttc agctcgtggg cgagctcggc ctcatttcag tcctgccgga cctccgccgc
     9361 gctctgcgcc gcagtcgcct gtcagagcga cccgtctacg agaccacgct cgagcagctc
     9421 gccccgagca gctccgaggc aaaagccttg cttgcttcgc tccgccaggc gaatcaatct
     9481 cggtaaggaa ctaccgatgc tcttcgacac gcattgccac ctcgacgatc cacgcctttt
     9541 cgacgagctc ggtgaggtcc tcgagcgcgc cgagcaagct ggggtgcgtc gtatcaccag
     9601 catcggctgc gcaagtgacg tcgaatcggt catgtccgca gtccggctcg cgaagcaaca
     9661 ccccaaccga atctcggcga cagtcggcgt tcatccgcac gatgcaaaac atctggacga
     9721 cgccctctac gatgcgatcc gggacgctgg ctccgatgcc tcggtggtcg ccatcgggga
     9781 aaccgggctg gatttccact acgaccactc cccacggcaa gcgcaggagg aagcctttag
     9841 aaaacaggtg acgatcgcca gggagctcaa gaaacccctc gtgattcaca cccgcgcggc
     9901 acccgagcaa acacttcgca ttctgaggga agaacaggca aaggacgtag gaggcatcat
     9961 tcactgcttc agcgaggacg cgccctttgc ggcggcggcg ctcgatctgg gtttcgtttc
    10021 ctcgttcagc gggattgtga cgttcaagaa ggcggtcgct gtccaggagg ccgccctcaa
    10081 gcagccctcg gatgcactgc tcatcgagac cgatgcaccg tatctggcgc cgatcccaaa
    10141 acgaggaaaa cgcaatgaac ccgcgtacgt agcgtacacc gcggcccgaa ttgcggagct
    10201 ccggggcgag gatcccgagc tgctcgccca gacgacatat caaaatgccc ttcgaatctt
    10261 cggtatcgcg gactgagcgg acgggccgcc tgctcgacgc tccgccttga ctcgcgactg
    10321 gtcatcacta tctttgcggc ccttttcccg gggagaacac ttgtgaatac gaatcctggc
    10381 ctcatcggca ccaagcttgg cactactcaa atcttcctcg acgacgggga ggtccggcgc
    10441 gtcactgcag tcaaggcggg accctgcgtc gtggtaggca agcgcaccct gagcaaagat
    10501 ggctacgcag cccttcaact gggctttgga agccgtcgcg agaagctcgt gagcaagccc
    10561 gaggcgggca acttcaagaa ggccggcgtg gatgcgcagc gagtcgttcg cgagtttcgc
    10621 atcgacgaat cgctgctcga gcgcttcgag gtcggccaaa ctctcggcgt cgccgacatc
    10681 tttcaggacg gccagttcgt ggacgtctcg gcaacgagca aggggcgcgg cttcaccggc
    10741 gtcatgaagc gtcacaactt cgcgggcgcc ggtacggtcg gccacggtac gcatgaggcc
    10801 aagcgccacg gcggttccat cggccagaac atgacccccg gccacacgcg tcgcggtcaa
    10861 aaaatggcag gccagtatgg caacaagaga gcgacgcttc tgaatctcaa gatcgtcaaa
    10921 gtgctctccg atgagaacat cgtgctcatc gagggcggcg tacccggccc gcgcaacggc
    10981 gtgatcacgg tgaaggccgc ggtgaaaaag atcgtcccgc cactgccaga gcgtgcagcc
    11041 aaggcagagg ccaacgtgcc tccaccggaa gcgaccgaag aggcctaact gggccctcgg
    11101 cgccagcagg atcaccacgg acgccgtgcg cggcgcgaag ggtacccggc tcgctccgtc
    11161 tacaagcttc aagagatcga tcggcgcgtg ggcctcttca agaaggggca gcgcgtgctc
    11221 gacctaggag ccagtcctgg ctcttggacg ctctattcgg cagagcaggt tggaccgacg
    11281 ggacgcgtgc tcggaatcga tctgaacgat ccggacgtgg cacttccacc gcaggccgag
    11341 atccgtacgc tcgacgcctt tgaagtcgac cccgcgagcc tgggtgggcc ttttgacctc
    11401 gtcatcagcg acatggcgcc gaagaccagc ggacagcgtc atgccgacca gtacagaagc
    11461 tacgagctcg tcatgcgcgc actcgagctc gcgaagaccg tcctcaagcc cggaggcacc
    11521 ttcgttgcga agatctttca gggcgccgag ttcgacgacg cccgcaagtc cctgcgcgac
    11581 tcgttcggca aggttcgaac catgcggccc gaagcgactc gagacgagag ctacgaagtg
    11641 ttcctcgtgg gaatcgactt caagccagct agttcttgag cgactcgaga cgggcgcgcg
    11701 cgcgctcggc gtacgggcca ccgggatcga ggctcacggc gcgacgcaaa tgcactcgcg
    11761 ctttgccgac ctgtccctgc ttcgcgagaa tcgatccata ggcatactga aggcccggat
    11821 cgagctcgtc ctgagctacg gcgcgccgca tcgtcgcctc tgcagaaccg aggtcgccgc
    11881 tcgcgtagta agctagcgaa agctcaccga gcagctcggt cggtggcggc tcgggcgcct
    11941 gttcgagcgc tggacgcaac acagaaaccg cgtcgtcggg gagtcctgct cgtcgaaaac
    12001 cctccccgac ggcgatggag agcaacacgt cccctcgcac catcggatgg accagacgaa
    12061 gctgtgcggc cgcatcatcg aagcgccgaa ggtcgattag catcatggcg agattgatcc
    12121 gggggacagg atcgttcggc aggtcttcga tcgtcgcaag gtactcacgc tcggcgtccg
    12181 ccatcttccc cagctcttcg taacaaagag ccaagttgaa gcgcgctggg gtgagatccg
    12241 ggtccaaagc gactgaccgc tcgagcgccg tggttgcctc cgcaagcttt ccggtctcac
    12301 gcaagagaac gcctgagttg ttgtacgcct cggcgaagtt ctgatcgagc tcggtggcac
    12361 gccggtatgc ggcttccgcg gaaccgaaat cgcctttggc ctcgtgcacc aaacccaggt
    12421 cgagccatgc ccgtggatcg gtcgggtcgt cggcaagcgc ctgctcgaat gcgcgcttcg
    12481 cctcgccaac cttcccttcc gaaaacagcc gctcgccgcg caagacctcg gggctcgcgg
    12541 gaggtcggct cacgactgac gcaggaggag actccttggc ttcgccctgt ccggacgcgc
    12601 acccaagcgc gagcgaaaag atggccgcac agcagagtcg cgcactcgtc acggcacctc
    12661 ggcacgcgcg tgcaggccga ggatgcgatc atagagcacg cgctggagtt tcttcagacg
    12721 ttccgggtca tgaaccttgt tgccgtcttc atcggagacg tagaagacgt ccgcaacgct
    12781 ctgcccctcc gtgttcacct tggacagggc gatggtcagg tccaattggt ggagcgtgcg
    12841 cgcgatctca tgaaggagac cgatccggtc gcgggtgaag acgtcgacga tcgtgtattg
    12901 agaggaggca ttgttgtcca cgctgatatg cgtcggaaca tcaggccccg gccgcacgga
    12961 ccagggcgga ggcgtgctct ggcgcgcgat gaaatcctgc gcggagacgc ggttggtgag
    13021 tcgatgatcg atgtcttctt ggagcgcttc ggcgagcctc gtgggcgttg gggtgttgtc
    13081 gccgaccttg atccaaaaca ggtcgaatgc ctcggcgccg ctcttccgcc ggcgggtgta
    13141 gagttgcgca gccaaaatcg aaagatcgtg ggcagcgaag acagccgtga aatctgcgag
    13201 aaggccggga cgatccgccg aaatcacgac cacttcttcc acgtcttcac tcggaccggg
    13261 ccccactcgc accgtggcta tgccactgga gcggtcccgg gaaatcctcg cgtgacgccg
    13321 aaccgcgtcg acagggtttg caaggaagta ccggtccggc atctcgtcga tgaagctctg
    13381 gagctcctcc tcgccagcat ctccggcgaa acccacgata gcctgcagct tcacttcgag
    13441 cactcggcct tcggtgtgcg ccggagcact ccctgactcg atctgggcga gcgttgctag
    13501 gtagaggtcc tcgagcgcac gcgccttcca agaggtcatt gcttttgggt tgaccgtcgc
    13561 gatgaccgac acggtgcaga ggaacagatc gcgaagccgc tcgggagttt ctacgatctt
    13621 gacgacttcg gtcagtccgc gcgggtcgtg aatgtcgcgc tgcgttgccc atcggtagag
    13681 cgatttgtgc tcccgaacca gccaggcgac gtgttgagcg tcgactgcac tgagccccaa
    13741 acggagtgcc acgctttcag cgagtcgggc gcccgcctcg tcctggtcgc gcccacgtgc
    13801 acgcccgagt gaatgcaaaa gcactgccaa gaagagcgac agtcgcctcg gggcctcggc
    13861 cgcgagtcga gcagaaagcg cgcgaccctg gctctcgcca cgggtgagcg cttgaagata
    13921 ctcgagcgct ttgaccgcgt ggatgtcggc cgtgtagacg tgaaacacgt catggtagac
    13981 gcggcccacc agaggctcga actccggaat catcgcggac accagcccaa cttcgtggag
    14041 ctctgcgatc gtcgagccac ttcggaacgg cgactcgtcg acatccttta ggagagccag
    14101 gaagagctgc gtcgcctcct ccgaaccttg aaggcggagt ctccaacgct tgtcgggggc
    14161 gattcgggcg atcgcgtcca acgcagagtg gtctggtcgc ttctgatatc gcaaggcctg
    14221 acgatagaat cgaaacgcga gcgcgggatc agcatctagc cgctgtggtt tttccaggct
    14281 gatggttcca tccgccaaca cgatcccatc ggcgagcttg cgaaacgtcg tagcgcgagc
    14341 gcgatttcga gggcgtgctc gatcgagcat ccgttccgcg gtgagcgcaa cgattcgcgc
    14401 atggcggtag tacgcctgca tgaactgctc gacgcccagc gtgacaccat cgacgaaccc
    14461 gagctcctga gcgatgtctt cctgatctcc aaacgtcagt cggtcttgct gccgtccggc
    14521 acggaggtga aggagattgc gaacgcgcca gagcatttca cgcgcggcgt cgagctctcg
    14581 aacctcgcgg gcgagaagcg cgcccgtccg aacgtagtct tgctcgttac gtgcgcccca
    14641 tcgggctcgt gcggcccatt ctgcgacgtc catgtcccgc aaccctcctc ggccctgctt
    14701 cacgtcgggc tcgagcaggt agaggctgcc accaaatcgc cgatgccgtg tctcggtgtc
    14761 ctgttccagc gcgccgagga actcgacgag gtacggttcg aagacctgtt cgcgggcccc
    14821 gcgctccaag tcgtcgagca gagagccgct tccggcgatc cgccggaagt cgatcaaagt
    14881 cgtcgtggtc cggatgtctt ctcgcgctag gcgaagtgtc tccccgacgc tccgaacagc
    14941 gtggccgatg tcgactccga ggtcccagag cggatacagc aggccttcgg ccagcgcagc
    15001 cacgaaaggg tcatccgagt cgtcgcagag gaagacgacg tcgacgtcac tgtgtaagcc
    15061 gagagtccgc cggccatagc ctccgacggc caccaaggcg accctcccac ccggctcgca
    15121 accttgtgcc cgcgtggctg cgtccgctgc gcagtagagt gctcccaaga gaccatcgag
    15181 tgccctcgcg tgcgcccggg ccgtgctgac gccggggtcc ccacggcgaa ccgcgtcctc
    15241 gaacgcccgg cggtatccac cgaggtaacc gctacaggtc ttgccgagcg ttggtgcgag
    15301 gccacctaga tcgacgaccg gactggatcc atccgacaac ggtcggcgag tatgactgcc
    15361 gccgtcgggc cgggcaaggt attcactcaa tgcgcctgca ggtacgaaac gatgcctgaa
    15421 gcaacaccgt cggccagggc cctgcgatag cttcgtttgg ccagcgcgcg cgcttccggc
    15481 tcataggtca ggaacgacgc ctcgaccagc acggccggca tccgggcgcc gaccagaacg
    15541 tagaacatcg cgcggcggac gccgcggtcg gaaagctccg gcagaaggga gcggccccgt
    15601 ttgagcgtgt ggcgttggat cttgtccgca agagtgagcg agccttctgc ttgggtctgc
    15661 cggtgatgct tcgcaagtag actctgaatc ccagtcacct ccgcgacgcg ggttccattc
    15721 tcccgggccg caagccggag tgcttggcgg ttgttcgtgg tgtcgagcac gaacgtcgtg
    15781 acaccacccc tacgaacctg ctccgagctc gcgttcagat gaatcgagac gaaagcgtcg
    15841 gcctccaatg agttggccat cgcgcagcgt tcttccagcg agagatcgac atcgcctgtt
    15901 cgcgtgagca tcacgcgggc gcggggcatg atctcgcgca accggtttgc cgtgagctct
    15961 gcgagctcga gcgcgatctg cgactcgacc agtccgtatc gatcatttgc cgcgccgggt
    16021 tcagggcctc cgtgtcccgg gtccaagacg accagcggcc tgtccgatct tcgcggacgg
    16081 cgatcggcgc tcacatcgac gacgatgcga tacggatcgg tgagatagaa gacacgcccg
    16141 gtcgcaccgg gtaggagatc caagacgacg cgggggttgg gcgtcgccaa gcgcacacgc
    16201 ttcaacccgg ctcgctcgac cgcctgggaa acgggcaatt cggcgcccca ctccgcatct
    16261 tgcagctcga tggagatccg agctggcatg acgccttgcg ggggcagctg cttttgttga
    16321 aacagcgggg cgccttcgaa aagcaacacc acgcgggcgc ctgctgcatc tttcgagtcg
    16381 accccgtaaa cgtcgatacg caccaggcgc gccttctcgc gggccggctc gaccgcctcc
    16441 ggaagctcgg ggagcggtcg cgggaggtcg cgatcggggc ttggaggcga gtcggagcgg
    16501 cagctcaagc tcaaggagag caacccgagc aagcagactt tccatgtgtt atccccgctc
    16561 cgaatgaagg cgtgtttacg cgacgctcaa gcgagcgtca acgtcgcacc gctcgttgac
    16621 ccgcaccgag cgatcgccta gcttccgcgg catggcgggc ctgacctatc gcgacgctgg
    16681 tgtcgacatc gaagccggcg agcaactcgt cgaacggatc aaaccgctcg cacaatcgac
    16741 acgctccgag cacgtgctcg gctcgattgg cggttttgca gggctctgtt cgctcccaag
    16801 cgacatcgaa gacccagttc tcgtctcagg gaccgatggc gtcggcacca agctgaagtt
    16861 ggcgttcgag ctcgagcgac acgacaccat cgggatcgac ctcgttgcga tgtccgtgaa
    16921 cgacatcatt accgtcggtg cgaggcccct gttcttcctg gactactttg cgacctcgaa
    16981 gctcgatgtc gaccaaggcg aagcggtgat caaggggatc gcagaaggct gccgacaagc
    17041 agagtgcgca cttctcggag gagaaacagc ggagctgcca gggttctacg cgaccggaga
    17101 gtacgatctg gctggcttcg cagtcggcgt cgtaagccgc aagcggatca tcgacggcga
    17161 gtcggtgcgc ttgggcgacc aggtggtcgg agtcgcctcg tcgggtttgc actcgaatgg
    17221 attctccctg gcaaggcgag tcctcttcgg gggtgatact cccctctcgg tccatgattc
    17281 cttggattcg ggcattgctc tcggagaagc cctgcttcgc ccgactcgga tctacgtgaa
    17341 ggctgctcga ctcgcgctcg accacgatgt gcacgcgatg tgtcacatca ccggcggagg
    17401 cctaccggag aacctacccc gcgtgacacc caaaggtctc ggaatcgaga tcgacccgag
    17461 ctcctggccc atcgatccca ttttcaccct aatccaagaa cgtggcagcg ttgcagatgc
    17521 cgagatgcgg cgcaccttca acatgggcgt cggcttcgtg atggtggtga accccggacg
    17581 agccaaagga ctggtcgatg cgctgagcgc cgcaggcgag aaggcattcg tcatgggcga
    17641 ggtcgtcgat cgcgaaggtg tttcgttctc cggatagagc cctgcatgga cgtcgtggtt
    17701 ctcgtttctg gtcgcggttc gaatctgagg gccatctgta cggcgatcga cgctggcgca
    17761 tgcgatgcaa agatcgtcgg cgtcgtctcc gatcgaaaga gcgcggctgc cctcgagttc
    17821 gcccaggcgc gaggagttcc tactaaagtc gttccgctgc gaaagtctga cgaccgcgac
    17881 cgctggaacg agctgctcgc gcaagaggtc gctcagctcg atccggaatt gatcgttctg
    17941 gcaggtttca tgcgcgtcct cggcaggccg ctgctcgacc ggtttcccgg tcgcatcatc
    18001 aacgtgcatc ctgcgctgtt gccctcgttt cccgggcatg acgggcccca ggatgcactc
    18061 gcgggcgggg ttcgagtgag cggatgcacc gtgcacatcg tggacgcagg cgtcgacacg
    18121 ggtccgatca ttgcacaggc cgccgtccca gtcctcgtgg atgacaccgc cgagacctta
    18181 catgcgcgaa tccaggttca agagcatcgg ctcttaccag gtgtgattca ccagatagcg
    18241 atcggcgcca tcacgctagc gcccctgacg gtgcgcaccg cggtcgctga ccctacccaa
    18301 tcgctgacgg ttccggacgc gtagattcgt gccaactagc ttctacgacg tcgtggtcct
    18361 gggaacgaat ctggagccgc tgctctgcgc cgcgctcttg gcacgtgaag gactccgcgt
    18421 gctcgtcctc gggcaggacg ttcctgaacc ctcgtacacc ctcgcaggcg tcgaagtgga
    18481 accgtacgcc atggtcgtcg cgggcgcgca gagccccgca gtggccaata ccttggacga
    18541 gctcgctctg cgacaggacc tgcgccagag gatcgtggaa cgcccgagcg tacttcagct
    18601 cctgttgccg gaacatcggc tcaacgtcca catggaaaac gagaagtggc ttgctgagct
    18661 cgaccgggaa cttccggacg tcagaggtca agccgtagac atcacgcgca cgctccgaga
    18721 ggtacgaacc gagatcgaca cgatggtgag ccggcggttg atttggccgc cggagacatt
    18781 catcgaacgg caacggttct cgctcgcggc caccacgcaa cgctatgacc ggctcgggct
    18841 cggttggacc tcgtggaacc aactacccaa gcggcatccg ttgcgtactg cgttcgaggc
    18901 aatgcttcct cacgtctctg gactagttcc aacgcagcat tccgacgcca cccgggcgcg
    18961 cctgcatggt catctgctct acggtgccac ggagctcagt gggggctggc cctggtttcg
    19021 cgaagccctc ttcgcgcgga ttcgttcctg gggcagtgat gttcgtcccc gagacagcgc
    19081 gagatccatc cagctccatg gtagccgcgg tcacacgatc cgcctcgccc gcagcgacga
    19141 ggagatcggc tgctcgcagg tggcccatgg gacctcgatc agcgagctct cccagttgct
    19201 gccggaccgc agttccatga cagcgatgtt cgaacgcgtg ggggagcctc gttcgcgcgc
    19261 ataccgctgc accgtccatg ttctcatcga cgcgcggggc gttcccaaag ccctcgaacc
    19321 ccttgccctg ctgtgcgcga gcgcgaccga tactggcaaa gcgttcctgt tgagaaccca
    19381 cgaggtggac gacgaacgaa tgctgatcag caccagcggc ctcatcgatg aacaccggat
    19441 cgacagcact tcatcacggc ttcaattcgt gcgcgaagaa gctctcgatg caatcaggag
    19501 cgtcattccg ttcctggacg agcatgcgat ttgggtcgac agccctcacg atgggctgcc
    19561 accgcgctcg ttgcgcggtg agggggagct ttcgtgcagt gatccctggg tcaggggtcc
    19621 gtcgacgatg aaatctgtct acgagttccc gacgcggagg gcgcttggcg tgtgtgccct
    19681 acccacacgc acccccgtgc ggggagtgtt cctctgtaac gatcagatcg caccgggtct
    19741 cggtttcgag ggatcgttct tggccgcaac ctcggtcgcc cggctcatcg gttctgagta
    19801 tcgccgacag gactggctgc gccggggccc gtgggcgcgt cgcagcgttt agggcatcgg
    19861 gtccaaccct ctgatatgct ttagcgcatg gcggcacccg aggaggcacc aaggctcaca
    19921 ctgcgacagt tgcttcgact gatggtcgag cgtggctcgt ctgatttgca cataacgacc
    19981 gggtcgccac cgcaattgcg tatcgacggc tcattggtgc cccttcgcac cgccccgctc
    20041 aagccgcggg agagcaaggc gctttgctac gaggtgatga ccgaagacca gcgcctccgt
    20101 ttcgaaaaag gacacgagct cgacttctcg tttggcgtca agtcgttgtc tcgatttcgc
    20161 gccaacgtgt ttcttcagcg cggcgcagtg tcaggcgcct tccgagcgat tccattcaac
    20221 atccgacccc tgtcggagtt gggtgttccg cccgtcgtcg aagagctctg cacgcgtcct
    20281 cgcggcctgg tcttggtgac cgggccgacc ggctccggga agtcgacgac gctcgcctcg
    20341 atgatcgaca agatcaacag cgagcaccgc catcacatcg tcactgtgga agatccaatc
    20401 gagtttctgc actcgaacaa gctttgcgtc gtaaaccagc gtgaggtggg ctctgacact
    20461 gcgggcttca aggacgcgct caaatacgtt cttcgtcagg atccggacgt cgtgctcgtg
    20521 ggcgaaatgc gagacctcga aaccatcgaa gcggcgctca cgatttcaga aacgggccac
    20581 ttggtcttcg caacattgca caccaacacc gcggtccagt cgatcaaccg tatcatcgac
    20641 gtgtttccct cgggccaaca gagccagatt cgggctcagc tttcattcgt gctccaaggc
    20701 gtcctgagcc aaatgctctt gccgcgcgcc gatggacccg gtcgagcgat ggcctgcgaa
    20761 gccctcgtcc cgaacgctgc gatccggaac ctcattcgcg aggacaagat tcatcagatg
    20821 tacggcatca tgcagaccgg ccagggatcc tcgggaatgc agaccatgaa tcagtcgctt
    20881 ttgcacctgg ttgcacacgg cgccctggac cgggagctgg ccgaaatccg ctcgccggaa
    20941 cccgacgaat ttcgccaaat gctcgtgcac ttagaccaaa agggctatca ccaagccatt
    21001 cgtgctgggg gctgattcgg cgtcccgcca gattttgact attgttaagg gcgggaattg
    21061 ggtgatggaa accacaaccg aacaccggcg ccggatagcg ctgctcgcct tcacgatatg
    21121 cttgttattt tccgctgtgg ccgcagcaaa cacggatgac cccggcggag ccgtttcgtc
    21181 gagcaacgtg cgaaagcggc cgaggcatac gaccagggca cctcggctta tctcaccgag
    21241 cgctacgagc tcgccgcaca ctggtttgaa cgagcctatc gactcgtgcc gacttcgacc
    21301 gccctactcc aagccatccg cgcgcacgac aaggctggca attcgattcg agcagccaat
    21361 ctcgcactgc aactgcgcga tgagtatccg aacgacgagc gatccagaga agttgcaaag
    21421 tccgtgatcg cggcgacgaa actcgacagc gttctcgttg aatcccactg cgaaaaagaa
    21481 tgcacgttgg agctcgatgg agctttgatt cagcacccga cgttcttcgt gactcccgac
    21541 gtagagcatt cgttgaaggc cgcgttcgac aacggcgaaa cctcgacgta cgtgcaaggc
    21601 gctgccggcg acgtcaagaa cgtgaccatc gctgcgccgg ccccacctcc tcctccgccg
    21661 gtgcctcgct gggcgttctt ctcatcgctc ggcgcgacgg ttgcgctcgg cgcagtgacg
    21721 gtttggtctg gcatcgacgc gaacaatggg gtgagcgcgt acgaatctgc tgcacgcacc
    21781 gccaactcgc ccggcatcaa tggcagcggc tctccgactc ccgagcagca agcgcaatcc
    21841 cttctcgacg agggtcagca gaaggagcgc cgaacgaaca ttctgatcgg cgttaccgcg
    21901 ggcatggcag ccagcacgat cgttctcggc gtgttcacca actggaaagg taagccgcca
    21961 gaaaccacag ccaaacgcct cgagccatcg attggagtcg gcgcgaaggg cggatcgatc
    22021 gcggtcaaag ggcggtttta gtgaacgctc ctgccagcgc tccgggacca gatcggttgt
    22081 cacgacccat ggcgggtcgg caactcggcc gatacgaaat cctaacgcag ctggcatcgg
    22141 gcggcatggc ctcggtttac atcgcgcgcg ctcaaggggt cgctggcttc gaacggcttg
    22201 tggcgatcaa agtgcttcat ccgcaccttg cttacgagca agagttcatc tcgatgttcc
    22261 tcgacgaggc tcggctcgcc gcccgcattc gccacatgaa cgtggttcct acgctcgaca
    22321 tgagcgacag ccccggtgac ggatactttc tggtgatgga gtacatcgaa ggcgaccacc
    22381 tcggagcctt gctcggctgt gctgccaaac agggcgaaag actcccgaag ccgttcgtct
    22441 gccgcgtgct cgtcgacact cttcagggac tggccgctgc tcaccggctc acggatgaga
    22501 acggaaatcc tttgaagctc gtccaccgcg acgtttcacc acacaacatc ctcgttggaa
    22561 ccgatgggat cgcgcggctc accgacttcg gcgtcgcgaa agccgacgtg agaatggcat
    22621 cgacacgagc cggccagttc aagggcaagc tttcgtacat ggcgcccgag caggcgtcat
    22681 cgagcgaaac ggaccagcgc tcggaccttt tcagtgtggg aatcattctc tgggagtgcc
    22741 ttacgggtcg gcgactcttc aagggagagt ccaacgccgc gacgctgaac aaacttctaa
    22801 acgatgcgat tgtgaagcct tccgttctct ggccggacct agagcctttc gatgctgtgg
    22861 tcatgaaggc gctgaaccgg gatcctgaac ggcgctttca gagcgcggac gagctcggtg
    22921 aggcgatcga cttggcatca gggcgcaatg gtatcgcgaa gacccgcgac gtcgcccaag
    22981 tggttcggac tctcgacgcc gagaagctgc aagaggaacg acaacgagtt cgtgacgcca
    23041 tcgagatgct cggtcgcgca gaattcgcgg aatccacgat tccgatgccc cgggaaggca
    23101 ttccggccaa aggcactagc attcggcatg gcagtgcaac gggctcctac atccatggga
    23161 gcgtctcggg tgttttggag gacggaaccc cggtagtcgt gcgacctgct ggggactcaa
    23221 atgcgctgaa gtggttcgtg atggccctcg ccgtgctggt cttcttgtat gccgggttcc
    23281 tcgtctggga gctcctccta acgccgtcgg cgcgacgggc tccggaaccg agccgtgccg
    23341 tcgaggtcgc ggcccccgta cccgtcgctc ccgaagagcc tcgggctacg aagagagccg
    23401 aacccggcgt cgaggtcgct ccagatccta cgagagaaac tgaacagatc gatggggtac
    23461 cggaacgcaa gagcagcgca gagccgtctc gacccgcccg gagacgggct cgggcgaaga
    23521 gccgcgccaa cgttccgcgt gcagccgagt ccaaaaaggc cgagaaccga gaggctgaat
    23581 cacctccgtc cgtcccggct tccaaagccg atccagtgga catcgacgtc aggaatccat
    23641 accggaacta gtcttgtaga aggtagtcgg agcggaattg ggcctcgctt ttgaccagtc
    23701 gatccaagat cagctaggat tttggtaggc ccatagggga ttgggcggag aattcatggc
    23761 cgagtgggta tacgaggcgc gcactcgcgc gggcgaggtc caaaccggga tcatcgaagc
    23821 ggacagcaag gaagccgtgc agtcacgact gcgcgcgcgc cagctcaatc cggtcaagat
    23881 caagaaaaag gggcgagagc tcaagctctc gttcggaacc ggtgtcacgc ccaaggagct
    23941 ggtcgtcttc acccggcagt tcgcaacgat gatcgacgcc ggtctgccgc tggttcagtg
    24001 cctcgatatc ttgtcgagtc gcggcgagaa caaggcgctc aatgcgatcc tcaaagacgt
    24061 caaggaccac gtcgagcaag gcggcacgtt ttcggagggc ctcgcgaggc accccaagct
    24121 gtttgatgag ctcttcgtca acctgattcg cgccggtgag atgggcggta tcctcgatac
    24181 cattctcaac cggctcgctg gctacatcga gaagcgagtc aagctcgccc gacaggttcg
    24241 gggcgccatg gtgtacccga tcgcgatctt ctgcgtagcg atcatcgtcg tcgtcgtgat
    24301 gctcgcctgg gtcattccga gcttcaaggg aatgttcgcc gagttcggtg gtgaagactc
    24361 cctgcctgcg ctcacgcaaa tcgtgatctc ggcatccgag ggcttcatgt ccaacgttca
    24421 ctggatcatc ttggcgaccg ttgggttcgc cttcggggtg agttgggtct accggcagcc
    24481 agccggaagg cacttctttc acaggacttt gctcgtgctt ccaattctcg gaccggtgat
    24541 gcgcaaaatc tcggtcgcac gtttcacccg aaccctcgga acgctgctct cggccggcgt
    24601 gccaattctc gactcgctcg acgtggtgaa gaagtcggcc ggcaacgtgg tggtcgagaa
    24661 cgcgattcaa cagacgtcgg acaaaatccg ggagggccgc acgatggccg agcctctcat
    24721 ggaaaccaac gtattcccac cgatggtcgt tcagatgatc ggcgtcggtg agcaaaccgg
    24781 cgccctcgac acgatgctca acaagatcgc ggatttctac gaagacgagg tcgatatcgc
    24841 ggtcgcggcg ctcacatcct tgctcgagcc gctgatgatg gtgttcattg gcggcatcgt
    24901 cggcgtgatc ctgatttcga tgtacctgcc gatcttctcg atcgctggta aagtcaacgc
    24961 ggattgaacc agacgcaaga acgcctgccc caagaccacg aggagcccaa tccgcatcgg
    25021 cggctgcagt gggtgctcgg cggccgactc atcatcgcga cagtccttct gggcgccacg
    25081 atgtatctgg cgctcgatgc aattcgctac gggcgcttca cgccgacctt cttgctgatc
    25141 ctgatctcgg ccatttacgc ctcatccatc gtgttcggcg tttggctgct caccggacgc
    25201 caagagaaca gggtcgccgt ggcgatcacg acattggatg tcttgttgat caccggcctg
    25261 gtgtacttga cgggaggtgc cggaagcatc ttttccttcc tctacggcgc acagatcctc
    25321 accgccgcgc tcaccttggg tacgaacgcc gcctattaca ccgcggcggc gagcctcgcc
    25381 tgttacttct tgcaaggcgt gtcgctcaac ttgggctggt tgcctccccc gcccgaccag
    25441 ccgctcagtc agtacgtgct cagctcacgg gacttccagc tggcattggt ttcaaacatg
    25501 gtggggatcg cagcggtggc gctgctggcg accaacctcg cgcgccgagc acaagtggcg
    25561 ggcgcgcgcc tcatcgaagc agaggaaagc gcggctcgac tggttcggtt gaacgacgac
    25621 atcgttcgtt cgatcgcatc tgggctgatc accgcgggcg acgatggact catcctcgga
    25681 atcaaccggg ccgcccgaga catcttgcgg gatcgagagg gatcgctcct cggacagtcg
    25741 ctctcggccg tccttccgaa ctacgccctg gaacgattga gcgagccgcc gaggcgtgcg
    25801 gacgcgatcg gtcgccgggc agacggtggc gagttcccga tgggatacac gctgagtgcc
    25861 cttttcgatg cggagggcaa tcgctccggg ttgttgctcg cattccagga tctcacggag
    25921 attcgcacgc tcagaaatcg ggcagagcga gcccaacgat tcgctgcact cgggcagctc
    25981 gcgactggcc tcgcacacga gatcagaaac ccgctgagcg cgatctccgg ttcggtcgaa
    26041 atggttcgcg agggcaatgc actcggcccg gaagatcggc ggctcctcgg tatcgtgatt
    26101 tccgaagtcg agcgtctgaa ttcgctggtc acgacgatgc tccaggtcgg gcgtccaggc
    26161 cagatcgagg cagagccgca cgatcttcgc tccgttgcaa gcgaagtcgt tgccatcgcg
    26221 cgcggcgaag cgaccgcaag caacggactc gagatcgaag cggtcgaccc ggggatcccc
    26281 atcatcgtct ccctcgatcc ggaccgcatg cgacaggtcg tttggaacct ggtgaggaac
    26341 gccgtacaag catcgccacg ccgcggcagg gtcgaggtcc gaaccgggcg cgacccctcg
    26401 ggctgtgcgt ttctagaagt cgcagacgaa ggacctggca tcggagcaga ccatcgcgag
    26461 cgtctcttcg acgtgttcta ttccggacga acccatgggg ttggactcgg gttggcgttg
    26521 gtgaagcaga tcgtcgacca acacgaagga cgcatcgaca tcgtcgatcg cgagggaccg
    26581 gggacttgct ttcgcgtgac gcttcctcgc ggcgagtctt cgctcagacc gaacgcagca
    26641 gcgcagccag acgagcaccc ggatccgccg agcgcatgag cccttcgccg atcagcacgg
    26701 cgtcggcgcg tcccttggca acctctcgga agtcctcgac gctctggaca ccgctcatga
    26761 aaaccgcgat gcgcccctca ggaattcgct cgatgcacag ctgagccgcg gccttatcga
    26821 ccgagaaggt gcctaagtcg cgcgcgttga cgccgatgat cgtcgacccg gccgccacgg
    26881 cccgatcgac ctcttcgcca ttggcggctt ccacgactgg ctccatgccg agctcccgga
    26941 ttcgcgccat gagcatccgg agatcggaat cttcgagggc gcgaacgagc agcaagacga
    27001 gcgaagcgcc aacgtcgtaa gccaggtcga cttggatcgg gtcgagcacg aagcccttga
    27061 agagcaccgg gacgctcacc gcttgcgcga cgcgtcgcac gagcaaaggc gaaccaccga
    27121 aacccggtcc gtcagcgaga acgctcacca cagcagcgcc accgcgttca tacccttcgg
    27181 caacccgaac gccttctcca ggtttctggg cgcgaatctc tcctgcgctc gggcttcgaa
    27241 acttcacctc tgcaatgacc gatggcgccc ctcccggcat gcgacgaagc gcccgaacgc
    27301 cccgctccga gcgctcaggc tgcgctatgc gactgaccgg gcgcattcgc gagcgatggc
    27361 gccgacgccg caggttttcg cggtgcgtgc gcgctagaat ggcggagagg tagtcggtca
    27421 tgggctttgc gtgagttgag cccaacgctc gagggtcact gctgcggctc cgctgtcgat
    27481 cgcttcggct gcgcgtcgcg cagcgtcgac tggatcccgt gcaagtccgg ccacccgcag
    27541 ggcggccgcc gcgttgagca agactgccac gcgcgcaggg ccctcatcgc cccgcaagac
    27601 cgcacggatg atctcggcgt tgcgcgccgc gtccccacct cggagtgctt caatcggtac
    27661 ttccggaagc ccgaaatcgc gcgggctgat ctcgaacgtg gtgacctccc cttcggccaa
    27721 ctgagcaacc ttcgtcgggc cggcgggaga aacctcgtcg aggccgcccg ccccatggac
    27781 gacccatgct ccggtcagtc ccagcgcccc gagcgcctgc gcgagctgct cgactcgtga
    27841 agcgtcatag accccgacca cctggtgcgt cgccgaagcc gggttcgaga gcggtccgag
    27901 caggttgaac atcgtacgaa tgccaagctc gcggcggacc ggcgccgcat gccgcatcgc
    27961 ggagtggtgg cttggtgcga acaagaagcc gatgcccact tcctcgatgc aacgctcgac
    28021 ccgctcaggc ggaagatcga tgcgcacgcc aagcgcctcg aggacgtctg cgcttccagc
    28081 tttggaggaa acagcgcgat ttccgtgctt agccacggct atgcctgacg cggccgctac
    28141 caaggccgcg gccgttgaaa tgttgaaggt atgcaagccg tcgccccctg tcccacaggt
    28201 gtcgagcagt ggcccatcaa cctgcggctg gatcgtctgg caatgcttcc gaagggcgag
    28261 cgccgcggct gcaatctcca cggcggactc acccttcatg cgaagcgcga ctacgaacgc
    28321 tgcgatttgg gctcccgagg ccttcccatc gagaatcaca tccatcgcgg cctcgacctc
    28381 gtccgcagac aagtcacggc ctgagacgag agcttggatc gcggtcgaga tcacgacttg
    28441 ccgctcgccg gaacggagga cagccagttg cgaatcagat catggccatg ctcggtgaga
    28501 aaggattcgg gatggaactg caccccttcg atcggaagct cgacatgacg aatgcccatc
    28561 acttcgtctt gatccgtcca agcagtgacc tcgagcgtgc tgggaatcgt gtctcgcttg
    28621 acgatcaagg aatggtagcg tgttgccgtc agcgggctgg ggaggtcttt gaacacgccg
    28681 cgaccttcgt ggtggatctg ggaagtgcgc ccatgcatga ggcgctcggc tcgtacgacc
    28741 gatgcgccga agtgttgtgc aatgctttga tgcccgagac agaccccaaa aatcggaatg
    28801 tccgcgccga gctcggcgat cgcgtccaag ctgaccccag cttcttcggg ccttccgggg
    28861 cctgggctga tcaggatgcc ctcgggacga cgctcgcgga tctcctcgac ggagaccgcg
    28921 tcgttgcgct caaccgcgac gtctgcgccg agctcgctca ggtactgaac gaggttgtag
    28981 gtaaaggagt cgtagttgtc gatcacgaga atcatcggtg tcgatcgcta gcggcttgct
    29041 gagagcgcgt caactcgcac ctgcaggcga cggttcgtag cctctctctc ggaagaactt
    29101 ctcgagcctc aagttgacca tctcgggata ctcgagggct gcgtaatggg tcgcgcccgg
    29161 aatcagcatg atctcggacc cgcgagttcg ccggaccatg cgctcggcgg ccgacctcgg
    29221 cgtgaatacg tcgcgggtcc cagcgatgac cagcatcggc acgtcgatca gcggaagcaa
    29281 atcccagcca tcgtgctctc cgagcttctc cagaagcctg aggaacgtat ccatgtccag
    29341 ggtgcggaac ttctcggcga gggctgcgac cagttgcacg tccagggtgc tcgccgcaag
    29401 gcccattcgc ttgatccacc cgcccggatc cggaacccgc atggcctgat gcaccacggt
    29461 ctcgatcgtg cgcggcatcg agcgcagacc gcgtagaaat ggcggcaaca agcgtcccac
    29521 cagattgagg ttgaagacgt agtcccaggg gcgcccggcc acgccgttga ccatcgcgag
    29581 tgaggccacg cgctcgggct cggcgcgaaa cacctcgagc gcgacctgaa cccccatgga
    29641 ccagcccacg agcgcagctc gctcggcgcc tacctcattg agcaggcaaa gggcatcccg
    29701 cgcgtgatcg gcaattgcaa gcgtcgcggg gtccgcgggg cgcgaggagt cataaagccc
    29761 acggtagtcc caggacagaa gccggtagcg gtcgcggaag tactgaatct ggtggctcca
    29821 ggcctcccat ccccctccga gcccattaat cagcaagatc gggcgccctt ggcccacgga
    29881 ctggtacgcg atttcagtgc catcaaatga cacaaagcgg cgttcttcga ctggtgatgg
    29941 catcatggca gccggcgaag cgtatcaggg gcagcttctc ggcgcttgcc ttgacaagtt
    30001 cggccccgca cggataaatt tgaggaagct gaaatgtcag acgaactcca aaaatccaag
    30061 aaagtccgcg atatacgcga gcgactcggc agaaacactg cggcaccacc gcctcagagc
    30121 ctcgaagaag ggggcgtggc gccaccgcca ccgacgctgg ggggagtggc accgccgcct
    30181 ggcttcgtcg gcgggctggc tgcaaagcgg tccggaccgt tcggcgccac cgctgcgaag
    30241 gcacctgcac aacaggaagt ccgcatcgtg gtcgacgagc aggccgtcgc caccgcacac
    30301 gagggcaaga gccggcgtgt gcgcaaattg atgatgatga ccggcggggg agccgcgctc
    30361 ctcggcttgt tcatcggaat gctggcgagc accgtgatgg gtaagcgcag cgagtacaac
    30421 atcgcagtgc gagacggaaa ggccgtctac gaaggcgttc gtgcggcggc tgaccaggtg
    30481 accgaagcaa agcggcttct cgatcgagcg atggcttcgg ccaaggcacg tcccggcgaa
    30541 ggcccctcgg ttgactacga agcgatcgag cagcttcgcg cccttcccac tccgtttacg
    30601 gctgccgatt tcaccggtct caactacacc aagttcaatc gtgagacggt caactcgctg
    30661 ttcgtctacg cacgtcaggt cggcgagctt tgggatcagt tcgatggtct cgccgggaga
    30721 gttctccccg agtcgcgtcg cgccgagctc gatgaggccg cacaagacga gactgcgatt
    30781 acgacgagcc caaccggttg tgtccccact ctgggagaag gtggatttcg ctgcggcctc
    30841 gtatacgtcg acatgccaga gaatgcggct gcgacgacgc tcaaggtccg cgctacccgc
    30901 ggcagcaaag ccttcgaaaa agagctctac atgggtcagg aactgcgcca aaacgccagc
    30961 aactacgtga tcctaacgaa tccggaacag tccgccggtg ttctcggtca acgcaagaat
    31021 gcgtttgcct cgtaccgccg cgacttggcc gaacttgggc gcctgatgga aacgactctg
    31081 gagacgcagg gcaccctcga aaagggcctg ggctcagtgg ccgggctcga ggagatcgtg
    31141 agcttctgaa ctgaattccc ctcgagggat cgaggggttc cgggtgcaga tctttcgacg
    31201 cgatcgatcc t
//
LOCUS       DASRZF010000404        11959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3403995, whole genome shotgun sequence.
ACCESSION   DASRZF010000404 DASRZF010000000
VERSION     DASRZF010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 11959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11959
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3403995"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2909
                     /locus_tag="VFG22_17965"
     CDS             <1..2909
                     /locus_tag="VFG22_17965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HET6418188.1"
                     /translation="PVEMALGGLPLSKGQRSISRYGLSSVTVLFESQADILQARQLVQ
                     ERLLDVAGSMPPGVDSPALGPLTGGLGEIYQFTVSSPDKSAAELLELVRFRIMPLLSA
                     VPGVVEVNSWGGERRTMDVVGNAARMARYRITLSNLTEATRKGTGYAAGATLDAGESG
                     VLLRGVAWPERASDLASTVVSMSEEGAPIRIGDVAEVVDSAVARIGAATTDGRGETVY
                     VMVQMLLEANALDVLDRVKSQMPKVRQALPDDVEIQVVYDRSELVHATLRTVFTNLLE
                     GGLLVVAVLFLLLGSFRAGALVASVIPLSMLGAVIGMVLLGVPGNLMSLGALDFGLLV
                     DGAVVMVEAIFHSTHERGGPIADRVEETATRMARPVFFAVLIIILVYLPILSLAGVEG
                     KMFRPMALTVLLALLSALILSLTFVPAMSRLFLRDQDIPTRPPFLVRWVSEKYGPILA
                     WAMGHPRQVAGSAVLLLLLGGTLAALAGAAFVPQLDEGDLVVQTVRDADIRVDSAIAD
                     NTRMEAAILEAVPEVLHIASRIGSPEVATDIMGLEQADIFIDIAPRAEWRSGVTRDQI
                     IREIQDAVASSAPAQEIAFTQPIQMRFNELVGGSVTDVSLSIYGEDLAALHDYASAVV
                     TEMAAVPGAVDVRVLAPPSVGLLEVRPRPLEASRFGFRSEDILEHVKAARAGIPAGTT
                     YDGPVQIPIRVRLATRTNALSFDRFELPTPSGALVALSQVAHVSRERGPALVNHEMAQ
                     RRVVVGMNVRGRDLGSVVQDAEALLGSPNLQPPEGVLLVWGGQFESLQSAKKRLNLII
                     PAVLVLMLSMLLILFRQLPPALLILLNVPFAAVGGIAALTARGLPISVSAVVGFIALS
                     GIAVLNGVVLITALMNELDKGVNAVEAMRRAALSRLRPVLMTALVAALGFVPMMLATG
                     VGAEVQRPLATVVVGGLLTSTLLTLVILPAIVPPVLALSARARAKATPFTTGAS"
     gene            complement(2915..3475)
                     /locus_tag="VFG22_17970"
     CDS             complement(2915..3475)
                     /locus_tag="VFG22_17970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418189.1"
                     /translation="MSKSPLSSERRLLIGVLIAFLGVAVFAAIDISADLHEGTAIGHV
                     LVEGGILLVALLGSIFMARRLVLTLRSARAAHEEVLALTEQLETSRADASRWRSEARD
                     LVRGLAEAIDQQFDRWSLTPAEKEVALLLLKGLSHKDIAEMRAVTEATARQQARAVYK
                     KGGLSGRHDLAAFFLEDLMLPVERES"
     gene            3598..4122
                     /locus_tag="VFG22_17975"
     CDS             3598..4122
                     /locus_tag="VFG22_17975"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HET6418190.1"
                     /translation="MDQLIFHPKVVHLPIALAVLMPLISGGVLLGWWRGWFDRRVWVL
                     VFSLQAALVGSGVLAMNTGEHEEERVEEAVAERHIEAHAEAAEAFVWASAALLVLMAG
                     PLVLKEGRARQAVSLGAFLGTLIVFGLGFRAGEAGGKLVYEYGAAQVYVTGDAGASPI
                     AGGDEAEDGDSDSE"
     gene            4196..5329
                     /locus_tag="VFG22_17980"
     CDS             4196..5329
                     /locus_tag="VFG22_17980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418191.1"
                     /translation="MRFIVALLALMFLSSTARAEGWHGSAAGGADFAFIPEVTGFGFV
                     LFEATGEDKVGKGDVRLFYNTDTIHLEIERIPLAKNLELSVALRGEAVFAGLLHQYYE
                     QGLRRSGLGFNASYLVLLPKLQWHFADHHTLELLTNVRYWIFGKNQGESQGYILPPDT
                     VVFEPRLGYIYWKIDSDGEEFRASKLFPRIKGVAAGASVGVDVRSTVQTWGFVADQPD
                     RNDPTKAIWTLNQWLRAGWKTGERFRLELQETFNWGWNQDDITRMRVGGMNPYVLVVP
                     GLPWSAVISERLAIGQLSGHIRPKKDKPQELGVLVSGGTVNDPYRQGLLEEFGGIGGL
                     ALFTDLRWGRWQVYARVGWAFPTAWLVDNPHVSGFVGLGVDAF"
     gene            complement(5342..5923)
                     /locus_tag="VFG22_17985"
     CDS             complement(5342..5923)
                     /locus_tag="VFG22_17985"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HET6418192.1"
                     /translation="MKHWIRNALPLALGVLLLSSSAFAAFSALKLDRVHSKVAFTAAT
                     LIFDVDGEFTDFTVNIDGDPAKPETVKVEANIDVASIDTKNQKRDDHLRSPDFFDTAK
                     YPKIRFASIGVKSEGDELIVKGNLTIHGKTKLVSVPFKVARGKNGAGIDTTTYKAKLT
                     IDRTEFGVGTESIAAKISLEDEVEVKLLIVTFQ"
     gene            complement(5998..6939)
                     /locus_tag="VFG22_17990"
     CDS             complement(5998..6939)
                     /locus_tag="VFG22_17990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET6418193.1"
                     /translation="MSPSDPSQARAYETHLRRATYASVLTALVLIAAKLAAWLWTGSI
                     SMLASLVDSLMDSLASAINLVAVRYSLAPPDAEHRFGHGKAEPLAGLAQAGFVSGSAV
                     FLLFHAIDRLRYPRPLRDIGVGIVVMVFSIFLTAALLTLQRRVIKQTQSTAIRADALH
                     YATDLLTNLAVVAGLLLASFGWGWADPVFAIMIAVYIFYSAIRIGLEGFQQLMDRELP
                     EDTQEKILSVAREHPEVLGVHDLRTRRSGRLRFVQLHLELDDHLPLTRAHAVADEVEA
                     RILTLLPNAEVIIHQDPATSTDEPGREPWRSDAGSEA"
     gene            complement(6961..8328)
                     /locus_tag="VFG22_17995"
     CDS             complement(6961..8328)
                     /locus_tag="VFG22_17995"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HET6418194.1"
                     /translation="MTTTFAALQLLLLLPMSWAVGRSDSSRSARTQASLVMLAAGALA
                     LAQIAWNTFDLPGPGWSEGFLPVSNPHPWVPAFSLISCLMGFAAVSLSPIRETRPKTF
                     RRILLLISVALLFLSVRSPIGLAVLWSASAVLTWLELRDLKNAPNIAKLFAIYHLPSA
                     AAFAIGAVCLTQGYVAAGLVLLLAAIAIREAVIPLHSWFPPFVQFAPMGVVVAFFAPQ
                     LGVYAHMELLRGALPEQLAHGVAFFGAVTAVVAAALGLAQSNARRALAFLVMSQTGLV
                     AFGLDTSSSVAQVGALLTWQVLGVATAGFAMTLASLEARRGDLSLRHPAGSFTRTPRM
                     AVAFLFMGFASVGLPLTVGFVAEDLLVQGSVEEFPVLAFALILATALNGVSVMRAFFK
                     LFSGSSEHIGEQDLTRREAGTLTVVMATLLLAGLFPGAAVRGLERVADPQHIRPDLAR
                     IESAD"
     gene            complement(8325..9692)
                     /locus_tag="VFG22_18000"
     CDS             complement(8325..9692)
                     /locus_tag="VFG22_18000"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HET6418195.1"
                     /translation="MSELIVRWAVLGIVGFPALAVLVLGLRAWLYHPWSERTTVGIVG
                     AGFAASTIACVVTTVSLLMAGVREARVPLGAWFSAGHYSFEWNLIADRLSIPFATFSA
                     VLLGLVASFARRYLHREPGFARFYLLLAIFGAAVEFVVLAGSLDLAFLGWELVGVTSA
                     LLIAFFHQRPGPVEHGLRAFVTYRICDIGLLAAGVALHFMVGTSDVGPLTSGPWAGFV
                     VPPTWAGATVVGLLILWASMGKAAQVPFSGWLPRAMEGPTPSSAVFYGALSIHLGPYL
                     LLRAESILQSSWLAASAVIAVGSLTALHATFVGRTQSDIKSALSYASMTQVGLIYVEI
                     GLGFRYLAVAHIVGHAAIRTLQILRSPSLLHDHHHLEQAMGKQLPRTGGHLERLVPDS
                     LQPWLYRVALERGGFDALLKDRIVAPLLRFICRIDRLDQAWVDYLSKPTVARQRKSQP
                     NEVLR"
     gene            complement(9689..>11959)
                     /locus_tag="VFG22_18005"
     CDS             complement(9689..>11959)
                     /locus_tag="VFG22_18005"
                     /inference="COORDINATES: protein motif:HMM:NF021578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HET6418196.1"
                     /translation="IAYWQMPGREGGLLDGFRGLYGLGSGPLDPWMRGLSSELRRQQS
                     EEWDAEQTILSALEAMGVAESQWDAYIEETLLSLRGWAGMIRQLELRPDTAPVAPPNA
                     RLADYLALQLMLDLHAARYALRTHAGESIDPAEASERLNDDTKQRQLNLELVYEAFIV
                     AQLAGVGPAEIGSSTQASRWLEAVRSFDSNERRRLLHLAYERRHRISVLDGIVGHCRS
                     GRVSPSRASFQAVFCIDDREESLRRHLEELRPDVQTFGYAGFFGIAMAYRGIEDIRAR
                     PLCPVAVRPSHYVYERALDEHEAAAFAERRRRQGKVTHSIGLGSKMLTRGGLLSALFG
                     FLAVIPLLGRSLFPRATERLAHWAVHRTAPVPETRLSVEGTGEVRPDGLASGYTLDQM
                     TNVVANVLTTMGIQKDLTSLVLIVGHGSSSLNNPHEAAHDCGATGGGRGGPNARAFAQ
                     MANDARVREQLRAKDLPIPDTTWFVGAYHNTCDDSMAYYDEDLLPKEASDALDDAKRA
                     FEDACRLDAHERCRRFESASLEIDPESALIHAEAHSYDLAQPRPEYGHATNAVCIVGR
                     RERTRGLFLDRRAFLVSYDPSEDPTGTILAGLLRAVGPVGAGINLEYYFSFVDPTGYG
                     CGTKLPHNIVGLVGVMDGHASDLRTGLPWQMVEIHEPVRLLTIVEAKTEMLERVLALE
                     EGLANLVNKEWIQFVAWDPDSDALAVYEDGKFHPYAVENPAIAVVDRSADYYSRRRGH
                     LSPAHVKAGLLERGVA"
BASE COUNT         2324 a         3699 c         3731 g         2205 t
ORIGIN      
        1 gtcccgtcga gatggcactt ggaggtcttc cgctttccaa agggcaacga agcatctccc
       61 ggtacggctt atcgtctgtg accgttctct tcgagagcca ggcggatatt ctgcaagctc
      121 gccagctggt gcaagaacgt cttctcgacg tcgccgggag catgccacca ggcgtcgatt
      181 ctcctgcact cggtccgctg acgggcggcc tcggcgaaat ttaccagttc acggtcagct
      241 cacccgataa gagcgcggcc gagcttctgg agttggtgcg gttccgaatc atgccgctct
      301 taagcgccgt cccgggcgtg gtcgaagtca attcctgggg tggcgaacgc cgcaccatgg
      361 acgtggtcgg aaacgcagcg cgcatggcga gataccgaat cacgctctcg aatctgaccg
      421 aggcgacgcg taagggcacg ggctacgcgg cgggcgcgac gctggatgcc ggcgaatccg
      481 gcgtgctgct ccgcggcgtc gcctggcccg aaagggcttc agacctagca tccacagtcg
      541 tctcgatgag cgaagaaggc gctccaatcc gcatcgggga cgtcgccgaa gtcgtggaca
      601 gcgctgtcgc acgaatcgga gccgcgacga ccgacgggcg gggcgaaacc gtctacgtga
      661 tggtgcaaat gttgctggag gccaacgcgt tggacgtgct cgatcgcgtc aagagccaaa
      721 tgccaaaggt tcgtcaggcc ctgcccgacg acgtcgagat ccaagtcgtc tacgatcgca
      781 gcgagctggt tcacgccacg ttgcggaccg ttttcaccaa tttgctcgaa ggtggtctgc
      841 tggttgtcgc cgtgctcttc ctcttgctcg gcagcttccg ggccggtgcg ctcgtcgcgt
      901 cggtcatccc cttgtcgatg ttgggggcgg tgatcggaat ggtcttgctc ggtgttcctg
      961 gaaatctcat gagtcttggc gcgctggact tcggtctctt ggtcgacggc gccgtagtca
     1021 tggtcgaggc catttttcac agcactcatg aacgcggagg ccccattgcc gatcgagtcg
     1081 aggagaccgc cacacgcatg gcgaggccgg tcttctttgc cgtcctgatc atcatcctcg
     1141 tgtacctgcc gatcctgagc ctcgccggcg tagagggaaa gatgttccga ccgatggcgc
     1201 tgacggtgct gctcgcattg ctcagcgcgc tcatcctgtc gctcaccttc gtgcccgcca
     1261 tgtcccgact gtttctacgg gaccaagaca ttccgacgcg acctccgttc ctcgttcggt
     1321 gggtctccga gaaatacggc ccgatcctcg cctgggcgat gggtcatccg cgccaggtcg
     1381 ctggatccgc ggtgctcttg ctgctgctcg gtggaacgct cgccgccctg gctggtgcgg
     1441 cattcgtccc tcagctcgac gaaggtgacc tcgtcgtgca gaccgtccga gacgccgata
     1501 tccgtgtgga cagcgcgatt gcggacaaca cgcgcatgga agcggcaatc ctcgaagcgg
     1561 tgcccgaggt gctgcacatc gcctctcgaa tcggcagtcc ggaggtggcg acggacatca
     1621 tggggctcga gcaagcggat atcttcatcg acatcgcccc gcgagcagaa tggcgcagtg
     1681 gtgtgacgag agaccagatc atccgggaga tccaggacgc tgtcgccagc tccgccccgg
     1741 ctcaggaaat cgccttcacg cagccgattc aaatgaggtt caacgagctc gtgggcggga
     1801 gcgtgaccga tgtctcgctg agcatctacg gagaagacct tgctgcgcta cacgactacg
     1861 ccagcgccgt cgtgacggag atggcggcgg tgccgggggc agtcgacgtt cgcgtgctcg
     1921 cgccgccctc ggttggtttg ctcgaagtcc ggccgcgacc gctcgaagcg agtcgatttg
     1981 gatttcgctc ggaagacatc ctcgagcacg tcaaagcagc gcgggcgggc atccctgcag
     2041 ggacgacgta cgatggtccg gtacagatac cgatccgcgt tcgtctggcc actcgcacca
     2101 acgccttgtc attcgaccga ttcgagctgc caacgccaag cggggcgctc gttgcgctct
     2161 ctcaggtcgc gcatgtctcc cgagagcgtg gacccgcctt ggtgaaccac gaaatggcgc
     2221 agaggcgagt cgtggtcggc atgaacgttc ggggcaggga tttgggctcg gtagtccagg
     2281 acgctgaggc gctcctcggg tctccgaacc ttcagccgcc ggaaggagtc ctactggtat
     2341 ggggcggtca gttcgaaagt ctgcagagcg caaagaagcg cctgaacctg atcattcccg
     2401 cggtgctcgt gctcatgctc tcgatgcttc tgatcttgtt taggcaactg ccgcctgcat
     2461 tgctgatcct cctgaacgtt ccctttgcag ccgttggtgg cattgcagcg ctgacagcgc
     2521 gaggccttcc gatctccgtg tccgccgtcg tcggattcat cgcattgagt ggcatcgccg
     2581 ttctcaatgg ggtcgttctc atcaccgcct tgatgaacga gctcgacaag ggcgtgaacg
     2641 ccgtcgaggc aatgcggcgc gcggccctct ctcgattgcg gcccgtgctg atgaccgcgc
     2701 tggtcgcagc tctaggcttc gtacccatga tgctcgctac gggcgtgggc gccgaagttc
     2761 agcggcccct ggctaccgtc gtcgtggggg gcttgctgac gagcactttg ctcaccctcg
     2821 tgatcctccc tgccatcgta ccgcccgtgc tcgcactgag cgcacgcgcc cgcgccaaag
     2881 caacgccttt cacgacgggt gcgtcctgac ggtctcagct ctcgcgctcg accgggagca
     2941 tcaggtcctc gaggaagaac gcagccaggt cgtgacggcc cgacaagcct cccttcttgt
     3001 agaccgccct cgcctgctgc ctggcggtcg cttccgtcac agcgcgcatt tccgcgatgt
     3061 ccttgtggct caatcctttg agcagaagca gagcgacttc tttttcggca ggcgtcaggc
     3121 tccaacgatc gaactgctgg tcgatggcct ccgcaagccc cctaaccagg tctcgcgcct
     3181 cgcttcgcca ccggctcgcg tcggcccgac tcgtctcgag ttgttcggtc aatgcaagca
     3241 cttcctcgtg agccgcgcgc gcgctccgca gcgtgagcac gaggcggcgg gccatgaaga
     3301 tggaacccag gagcgccacc agtaggattc cgccttcgac gagaacgtgt ccaatggcgg
     3361 tgccttcgtg gagatcggcc gaaatgtcga tggccgcaaa gactgcaacc ccgaggaacg
     3421 cgatcaagac gccgatcagg aggcgccgtt cggagctgag gggcgatttc gacatcgatt
     3481 ctatccgccg gatgtagcac ggcacccgac caagcgctgg agatcattcg catatgacat
     3541 ccgcagcatg gcgcgagggc atcgcttgcg cccatcgtac gctcggaggt taccaccatg
     3601 gaccagctca tctttcatcc caaagtcgtt cacctgccga tcgcgctcgc cgtcctgatg
     3661 ccattgatca gcgggggcgt tctactgggt tggtggcgcg gttggttcga ccgccgtgta
     3721 tgggttctcg tgttctcgct gcaagcggct ctggtcggat cgggcgtact cgcgatgaac
     3781 accggagaac acgaagaaga acgggtcgaa gaggcagtcg ccgaacggca catcgaggcc
     3841 catgcggagg ccgcggaggc gttcgtctgg gcgagcgctg cgctcctcgt cttgatggcc
     3901 gggccgctgg ttctgaagga gggcagagca cggcaagcgg tctccctcgg tgcgtttctc
     3961 ggcacattga tcgtcttcgg actgggtttc agagccggag aggccggagg aaagctcgtc
     4021 tacgagtacg gtgcagccca ggtctatgtc acgggagatg cgggcgcgag cccgatcgca
     4081 ggcggcgacg aagcagaaga cggggacagc gacagtgaat agaaccggcg cagcggatct
     4141 gcagcgaccg tgctcgggca tatgcaatgc acagccgctg cagtacgctt tggccatgcg
     4201 gttcatcgtt gcactgctcg ccctgatgtt cctctcatcc accgcgcggg cggagggctg
     4261 gcacgggtcg gcagccggcg gtgcggactt cgccttcatc cccgaggtaa ccgggttcgg
     4321 attcgtgctc ttcgaagcca ctggcgaaga caaggtcggc aaaggcgacg tccgtctctt
     4381 ctacaacacc gatacgatcc acttggagat cgagcgcatt cccctcgcaa agaacttgga
     4441 gctctcggtg gcccttcgcg gggaagcggt tttcgcgggc ttgcttcatc agtactacga
     4501 gcagggtctg cgcagatcgg gtctggggtt caacgcgagc tacctggtgc tcttgccaaa
     4561 gctgcaatgg cacttcgcgg accaccatac cttggagctg ctcaccaatg tccggtactg
     4621 gatcttcggg aagaaccagg gcgagtcgca aggctacatt cttccgcccg acaccgtagt
     4681 cttcgagcca cggctggggt acatttattg gaaaatcgat tcagacggag aggagtttcg
     4741 ggcatcgaag ctcttcccac gcatcaaggg cgtcgctgcc ggtgcatcgg tcggcgtgga
     4801 cgtccgctcg acggtacaaa cctggggatt cgtcgccgat cagccggacc gcaacgatcc
     4861 gacaaaggcg atctggacct tgaatcagtg gctgcgtgcg ggctggaaga ccggcgagcg
     4921 cttccgcctg gagctccagg agacgttcaa ctggggatgg aaccaggacg acatcacgcg
     4981 gatgcgggtg ggaggaatga acccctacgt gctggtcgtt ccgggcctgc cctggtccgc
     5041 ggtcatttca gaacgcttgg cgatcggaca actgagcggc cacatccgac cgaagaagga
     5101 caagccacaa gagttgggcg tcctcgtttc ggggggcacc gtcaacgacc cgtatcgcca
     5161 gggtcttctc gaggagttcg ggggaatcgg cggcctggct ttgtttacag atcttcgctg
     5221 gggccgctgg caggtctatg cgcgcgtggg ctgggccttc ccgacggcat ggctcgtcga
     5281 caacccacac gtctcgggtt ttgtgggcct cggcgtcgat gcattctgag tctcgcccag
     5341 gtcactgaaa cgtgacgatg agcagcttga cctcgacctc gtcctcgaga ctgatctttg
     5401 cggcgatgct ctccgttcca acgccgaact cggttcgatc gatggtgagc ttggctttgt
     5461 aagtcgtcgt gtcgatccct gctccgttct tgcccctggc gaccttgaac ggaacgctga
     5521 ccaacttggt cttcccatgg atggtcaggt tccccttcac gatcagctcg tccccttcgg
     5581 acttcacccc gatcgatgca aaccggatct tcgggtactt cgccgtatcg aagaagtccg
     5641 ggctccttag atggtcgtca cgtttctgat tcttcgtgtc gatggaggcg acatcgatgt
     5701 tcgcctccac tttcaccgtt tcgggcttcg ccggatctcc atcgatgttc acggtgaagt
     5761 ccgtgaactc gccgtccacg tcgaagatca gcgtcgcagc ggtgaaagcc accttgctgt
     5821 gaacccggtc cagcttgagc gccgaaaacg ccgcgaacgc agacgagctg agcaacaaaa
     5881 caccaagagc cagcgggagc gcgttccgaa tccaatgctt catgtggttc ttcctttcgt
     5941 acacggccac cggtacgtgc cacgcgccaa gatgtttcgg ccgacccacc tgtttgctca
     6001 agcctcactc cccgcgtccg agcgccaggg ctcgcgcccc ggctcatcgg tcgaagtggc
     6061 cgggtcttgg tgaatgatga cctcggcgtt tggcaggagg gtcaggatgc gcgcttcgac
     6121 ttcgtcggcg acggcatgtg ccctggtgag aggcaagtgg tcatcgagct cgagatgaag
     6181 ctgcacgaat cgcaggcgac cggaacgtcg cgtgcgaagg tcgtgaaccc cgagcacttc
     6241 tggatgctct ctcgcgaccg agagtatctt ctcctgggtg tcctcgggca actcgcgatc
     6301 catcagttgc tggaaccctt ccagaccgat gcgaatcgcg ctgtagaata tgtagacggc
     6361 gatcatgatc gcgaaaaccg gatccgccca gccccagccg aaagacgcca gcagtaggcc
     6421 agcaacgacc gcgaggttcg tcagcagatc ggtcgcatag tgcagcgcat ccgcccgaat
     6481 cgcggtggac tgggtctgct tgatcacccg gcgttggagt gtaagcagcg cggccgtcaa
     6541 gaagatggaa aacaccatca cgacgatgcc gaccccgatg tcgcgcaaag gtcgcggata
     6601 gcgcaggcga tcgatcgcat gaaacagcaa gaacaccgcc gaaccgctca cgaaacccgc
     6661 ctgagcgagg ccagccagcg gttctgcctt cccgtgacca aagcggtgct ccgcatccgg
     6721 aggtgcgagt gagtaccgaa cagccaccaa attgatcgca gacgcaaggc tgtccatcag
     6781 cgaatcgacc agtgacgcaa gcatgctgat cgaaccggtc cagagccaag ccgcgagctt
     6841 cgccgcgatc aggaccagcg ccgtgagcac ggaggcgtac gtggcccggc gcaagtgcgt
     6901 ttcataggcc cgcgcctgac tcgggtctga agggctcatc tctccgagga tatcatttcc
     6961 tcagtcggcc gactcgatac gcgcgagatc gggtcggatg tgctgtggat ccgccacgcg
     7021 ctcgaggcca cggacggccg ctcctggaaa caagcccgcg aggagcaagg ttgccatgac
     7081 cacggtgagt gttcccgctt ctcgtcgcgt caagtcctgt tctccgatgt gctcggacga
     7141 accgctgaag agcttgaaaa acgctcgcat gacggatacg ccgttgaggg cagtcgccaa
     7201 gatgagggcg aaggcaagaa ccggaaactc ctctacggaa ccctgaacga gcagatcttc
     7261 cgccacgaat ccgaccgtta gagggagtcc gacgcttgca aagcccatga acaagaacgc
     7321 tacggccatc cgaggtgtcc gcgtgaagct ccccgccggg tgccgaagcg aaaggtcgcc
     7381 tcggcgcgct tcgagggatg ccagggtcat ggcgaaccct gccgtggcca cgccgagcac
     7441 ctgccaggtc aggagcgcgc ccacctgtgc aaccgaggag ctcgtatcca gaccgaacgc
     7501 gaccagaccg gtctgactca tgaccagaaa tgcgagcgcc cggcgcgcat tgctttgcgc
     7561 tagacccaag gctgccgcca ctactgctgt gaccgcgcca aagaaggcta cgccgtgagc
     7621 gagttgctct ggcagcgctc cacggagaag ctccatgtgc gcgtagacgc cgagctgtgg
     7681 tgcgaaaaag gcgaccacca cccccatggg cgcgaactgc acgaacgggg ggaaccagct
     7741 gtgtaaggga atgaccgcct ctcgaatggc aatggccgcc aggagaagca ccaggcccgc
     7801 agcgacgtag ccctgcgtga gacaaacggc gccgattgcg aacgcggcgg cactcggcaa
     7861 gtgataaatc gcgaacagct ttgcgatgtt cggagcgttc ttgaggtctc tgagctccag
     7921 ccaggtcagc acggccgaag cgctccacag gaccgcaagt ccgatggggc ttcggacaga
     7981 gagaaagagc aatgcgaccg agatgagcag caagatcctg cgaaacgtct tcgggcgggt
     8041 ctcgcggatg gggctgagcg aaaccgcggc gaaccccatg aggcacgaga tcagcgagaa
     8101 ggccggtacc cagggatgtg gattcgagac gggcagaaat ccttccgacc aaccgggacc
     8161 cggaagatca aaggtgttcc aggcgatttg cgcgagggcg agcgccccgg ctgcgagcat
     8221 gacgaggctt gcttgagtgc gcgctgagcg cgacgagtcg gaacgcccga ccgcccatga
     8281 catcggaagg agaaggagta gctgcaaagc cgcaaaagtc gtggtcatcg gagcacctca
     8341 ttgggctggc tcttccgctg cctcgcgacg gtcggtttgc tgaggtagtc gacccaggcc
     8401 tgatcgagcc gatcgatccg gcagatgaag cggaggagtg gggctacgat cctgtccttg
     8461 aggagcgcat cgaatccccc gcgctcgagg gccacccgat agagccaggg ctgaagagag
     8521 tctggaacca agcgctcgag gtgtccgccc gtccgcggca gttgtttgcc catggcctgt
     8581 tccaagtgat ggtggtcgtg gagcaggctc ggtgaacgca aaatctgaag cgttcggatc
     8641 gcagcgtggc ccacgatgtg cgccaccgcc aagtagcgga atccgagacc gatctcgaca
     8701 tagatcaatc caacttgcgt catcgacgca taggacaatg cgctcttgat gtcgctctgt
     8761 gttcgtccga cgaacgtggc gtgtagtgcc gtcaacgaac ccacggcgat gacggcgctt
     8821 gcagcgagcc atgaagactg caggatcgat tcggcgcgaa gcaagagata gggtcccaga
     8881 tggatcgaca gcgccccgta aaacaccgcg ctcgatggag tgggaccttc catggcgcgc
     8941 ggaagccatc ctgagaaagg aacttgagcc gccttgccca tcgacgccca gaggatgagc
     9001 agacctacca cggtcgctcc tgcccaggtc ggtggaacca cgaagccagc ccacggtccg
     9061 ctggtgagcg ggcccacgtc ggacgttcca accatgaaat gaagagccac cccggcggcg
     9121 agcaacccaa tgtcgcaaat tcgataggtg acgaatgctc gaagcccatg ctcgactggc
     9181 cctggccgtt ggtgaaagaa ggcgatcagc aaagcggatg taacgcccac gagctcccaa
     9241 cccaagaacg ccagatccaa gcttcccgcc aggactacga actccacggc cgccccgaag
     9301 atcgcgagca gcaagtaaaa cctggcaaag cccggctctc gatgcaggta tcgcctcgca
     9361 aatgaagcaa cgagtccgag caatacggcg gagaaggtcg cgaacgggat cgacaaacga
     9421 tctgcgatca ggttccactc gaacgagtag tgcccggccg agaaccatgc accgagtggt
     9481 acgcgtgctt cgcgcacgcc ggccatcaac aacgacacgg tcgtgaccac acaagcaatc
     9541 gtcgaagcgg cgaagccagc ccctacgatc cccaccgtcg ttcgctcgct ccaaggatgg
     9601 tagagccagg cgcgaaggcc caagaccaac acggccagag ctggaaatcc gacgataccg
     9661 agtaccgccc agcgtacgat gagctcgctc atgcgacacc tcgctcgaga aggcctgcct
     9721 tcacatgggc cggactcaaa tggccgcgtc tccgagaata atagtccgcc gatctgtcga
     9781 ctacggcgat cgccggattc tcgacggcgt aggggtgaaa ctttccgtct tcgtagaccg
     9841 ccagggcatc gctatccgga tcccaggcga cgaattgaat ccattccttg ttgaccaggt
     9901 tcgcgagccc ctcctctagg gcgagcacac gttccaacat ctcggtcttc gcttcgacga
     9961 tcgtgagaag tcgaacgggt tcgtggatct ccaccatctg ccaagggagg ccagttctca
    10021 gatcggacgc gtgtccgtcc atcacgccga cgagcccgac gatgttgtga ggcagcttgg
    10081 tgccgcaacc gtaccccgtg ggatcgacga agctgaagta gtattcgaga ttgatgcctg
    10141 cgcccactgg tccaacggct cgcagcagcc ccgccaagat cgtccccgtt ggatcctcgc
    10201 tgggatcgta agaaaccaga aatgcacggc ggtcgagaaa caggccgcgg gttcgctccc
    10261 ttcgacccac gatacagacg gcgttcgtcg catgtccgta ctcgggtcgg ggttgagcga
    10321 ggtcatacga atgcgcttcg gcgtggatca gcgcggactc tggatcgatt tccagagaag
    10381 ccgactcgaa ccggcggcat cgttcgtgcg catccagacg gcaagcgtcc tcgaacgccc
    10441 gcttggcatc gtccaaagcg tcgctcgcct ccttcggaag caagtcctcg tcgtagtaag
    10501 ccatggaatc gtcgcaggtg ttgtgatagg cgccgacgaa ccaagtcgtg tccggtatcg
    10561 gcagatcctt cgcgcgaagt tgctctcgaa ctcgggcgtc gttggccatc tgagcgaatg
    10621 cgcgtgcgtt cggccctccc cgaccgcctc cggtcgcgcc acaatcgtgg gccgcttcgt
    10681 gtggattgtt gagactggac gagccgtgac caacgatcag aaccaaggag gtgagatcct
    10741 tttggatccc catggtcgtg agcacgttcg ctacgacatt ggtcatttgg tcgagagtgt
    10801 acccggacgc cagtccgtcg ggacgaacct cgccggttcc ctcgactgag aggcgcgtct
    10861 ctggaacggg cgccgttcta tgcacggccc agtgagcaag gcgctcggtg gcgcggggaa
    10921 acaggctcct gcccagcaac ggaatcacgg cgagaaagcc aaagagcgcg gacagcaggc
    10981 caccacgcgt gagcatcttg ctgccaagtc cgatggaatg ggtgactttt ccctgacgcc
    11041 gtcgtctttc cgcgaaggcc gcagcttcat gttcgtcgag cgcccgctcg taaacgtagt
    11101 gactgggacg cactgccacc gggcaaaggg gcctcgctcg gatgtcctcg atccctcggt
    11161 aagccattgc tatcccgaag aagccagcgt agccgaacgt ttggacgtcg ggacgtagct
    11221 cttcgaggtg cctgcgaagc gattcctctc ggtcatctat acagaacacc gcttgaaagc
    11281 tagctctcga cggagacacg cggcccgacc gacagtgacc cacgatgcca tcgagcacac
    11341 tgatccgatg ccgacgttcg taggcgagat gcagaagtct tctgcgttcg ttggagtcga
    11401 aagatcggac cgcctcgagc catctgctcg cctgcgtaga gctgccgatc tcggcgggtc
    11461 cgactccggc caactgagcc acgatgaaag cttcatacac gagctcgagg ttcaactgtc
    11521 gctgcttcgt gtcgtcgttg aggcgttcgg aagcctctgc ggggtcgatc gactcgccgg
    11581 catgcgttcg aagcgcgtac ctcgccgcat gcagatcgag catcagctgg agggcgaggt
    11641 agtctgcaag gcgggcgttt ggcggtgcga cgggagcggt atccggacgc aattccaact
    11701 gccgaatcat gcccgcccat ccacgaagag agaggagcgt ctcttcgata tacgcgtccc
    11761 actgcgactc ggcgacgccc atcgcctcga gcgcggaaag tatcgtctgt tctgcgtccc
    11821 actcctccga ctgttgacgt ctcagctccg aggacaggcc acgcatccaa gggtcgaggg
    11881 gtcctgagcc caggccatag aggcctcgaa atccgtcgag caagccgcct tcgcgcccgg
    11941 gcatttgcca gtaggcgat
//
LOCUS       DASRZF010000405         3497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_651857, whole genome shotgun sequence.
ACCESSION   DASRZF010000405 DASRZF010000000
VERSION     DASRZF010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651857"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="VFG22_18010"
     CDS             <1..1098
                     /locus_tag="VFG22_18010"
                     /inference="COORDINATES: protein motif:HMM:NF027980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol-specific phospholipase
                     C1-like protein"
                     /protein_id="HET6418197.1"
                     /translation="CVSVACSSDPDPGSEQDQTLRINEIQVLGTHNSYHIQPRDEILE
                     LLRIFDTPETADSLEYTALPLQEQFDHGVRQIELDIFADPEGGLYATRRGLILVDGAN
                     PESGIPELDEPGFKVLHIQDIDFETHCLTFHACLTTLKGWSDSHHKHVPITVMLEAKE
                     EAISDPLDLGFVIPLEFGPEEVADIDREILAVFPSEQLITPDDVRGEEDSLEAAVLNG
                     GWLTLAEARGRVMFVFLNRSAARDHYIDGHPSLRGRVMFTNSEEGEAEAAWFNVNNAL
                     DDGERIRELVEAGYMVRTRADEDTHQARDNDYSLQEAAFASGGQFVSTDYVVPVPDFG
                     NDYFAEVPGGYVARCNPISAPADCDSELIRP"
     gene            1280..2197
                     /locus_tag="VFG22_18015"
     CDS             1280..2197
                     /locus_tag="VFG22_18015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418198.1"
                     /translation="MISLLAHVIALVAIGVDTRAVTTAETTSIDIRFSVAADPQSAAP
                     SHTVVEEPAARADPERAAPQKSRAAVPESSLTAASRKAAVQVEIGLPSREASVPLLNL
                     DPSSAARAFHLSQQTVPQKASGSEPLAEDADSESRNYFEGAGDKKHLSRREPPKLQRH
                     RDGSYRYRGHAFKATIANDGTVEFDDGYQQGRKIAFDVTDMLMRRRGEDPYRVEKRWF
                     LDSTEGLREELLELWRVKRERHSLLKLRGRLNRVAEDRTLSDQQKAARVVSMYQATTD
                     DDTGIAARDVISEFVAESMPGIVLPNRTD"
     gene            2292..3221
                     /locus_tag="VFG22_18020"
     CDS             2292..3221
                     /locus_tag="VFG22_18020"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET6418199.1"
                     /translation="MSDQIKSGFLPIRAVVARTGLNPDTIRAWERRYGAIEPDRSAGG
                     TRQFSEEDVTRLLCLKELRERGFSLQTIAQLPTAELKSMRSGDREAIPRVRPTQPPLS
                     DQQQAYLDAIENLDANGALEQLHAMSEGIGNLQLILGVAIPIMWEVGDRWSQGTLGTA
                     PEHLVRAQLKSLMTARFEQIPNPVGARKLLFTTPAGQLHEFGILAGSVLAKEAGFDVL
                     YLGPNLPTEDIIWSVAKSQADILVLSVVRRMDNEEIRQMERLVDTIAPTVPVWIGCPP
                     EHPLIARTPKATFISSIEDFQLAASSLLSRAPN"
BASE COUNT          724 a         1090 c         1054 g          629 t
ORIGIN      
        1 tgcgtctcgg tcgcctgcag cagcgatccg gatcccgggt cagagcagga tcaaacgctg
       61 cgaatcaacg agattcaggt gctgggcacc cacaacagct atcacattca gccgcgtgac
      121 gagattttgg agttgctcag gatcttcgac actccggaga cggcggacag cctcgagtac
      181 acggcccttc cgctgcaaga gcagttcgac cacggcgtcc gacagatcga gctcgacatc
      241 ttcgccgatc cggaaggggg actgtacgcg acccggcgcg gtctgatatt agtcgacggg
      301 gcaaatccgg agtccggcat tccggagctg gacgagccgg gcttcaaagt cctgcacata
      361 caggacatcg acttcgaaac ccactgcctc acgttccacg cctgcctgac cacgctcaag
      421 ggatggtccg atagccatca caaacacgtg ccgatcaccg tcatgctgga agccaaagaa
      481 gaggccattt ccgatcctct cgacctaggc ttcgtaatcc ccttggagtt cggccccgag
      541 gaagtcgccg acatcgaccg ggagatcctt gctgtgttcc cctcagagca gctcatcaca
      601 cccgatgatg tgcgcgggga ggaggactca ctcgaagcgg cggtgttgaa cggaggatgg
      661 ttgaccctcg ccgaggccag aggacgagtg atgttcgtct ttctcaacag gagcgcggcg
      721 cgagaccact acatcgatgg gcacccttca ctgcgagggc gagtgatgtt caccaactcc
      781 gaagagggtg aagcggaagc tgcatggttc aacgtgaaca acgcgttaga cgacggtgaa
      841 cgcatacgcg agctcgtgga agctggatac atggtgagga cacgcgccga cgaagacacg
      901 catcaagcgc gcgacaacga ctactctttg caggaagcgg cctttgcgag cggcggccaa
      961 ttcgtcagca ccgactatgt cgtccccgtc ccagacttcg gcaacgacta ctttgcggag
     1021 gtgcctggtg ggtacgtcgc gcgctgtaac ccgatctccg cgcctgcgga ctgcgactcc
     1081 gagctgataa ggccctagcc tgggctcagc tgccataggc caaggcaggt gctggacgca
     1141 cctcatcgcg ggaagtaggt tcttcgccga cgatcaaccg agcgcgactg gttcgaagtg
     1201 atcgtcttgc agcactaaga gctttctcat tttgtcacga cccaaacgcg aaccagcacg
     1261 acgatggatg gctgtcctcg tgatctctct cctggcgcac gtcattgcgc tcgtggcgat
     1321 cggcgtggac acccgtgcgg tcacgactgc cgagacgacc tcgatcgaca tcaggttcag
     1381 cgtggcggcc gatccgcaat ccgccgcacc tagccacacg gtagtcgagg agcccgccgc
     1441 tcgggcggac ccggagcgag cggcgccgca gaagtcgcgc gcagccgtcc ccgagagctc
     1501 gcttactgcg gcgagccgga aggccgccgt gcaggtcgaa atcggattgc caagccgaga
     1561 ggcgagtgtg ccactcctga acctcgatcc atcgagcgcc gcacgcgcct ttcacctctc
     1621 gcagcagacc gtgccgcaaa aagcttctgg ctcggagccc ctcgcagaag atgctgattc
     1681 cgaatctcgc aattacttcg aaggtgctgg tgacaagaag cacctcagcc ggcgtgaacc
     1741 acccaaacta cagaggcatc gggacggctc ctaccgctat cgtggtcacg cgttcaaggc
     1801 gaccattgca aacgacggga cggtcgagtt cgatgatgga tatcagcaag ggcgcaagat
     1861 cgcgttcgac gtgaccgaca tgttgatgcg gaggcgtgga gaggacccct atcgggtcga
     1921 gaagcgttgg ttcttggaca gcacagaggg gctccgcgag gagttgctcg agctctggcg
     1981 cgtcaagcgc gagcgccact cgctgctcaa gctccgtggc cgtctcaacc gagttgctga
     2041 agaccggaca ctttccgatc aacaaaaggc ggctcgtgtc gtctccatgt accaggccac
     2101 gacggatgac gacacgggga tcgcagctcg cgacgtgatc tcggagttcg tcgcagagag
     2161 catgccgggc atcgtgctcc cgaatcggac cgactaagcg aggtcccgcc ctggcatctg
     2221 ggttctagtc ggggtcagga ccaattcatg gattgcacgg cgcggatttc ctgtgcatgt
     2281 acaggattgc cgtgtctgat cagatcaaat ctggattctt gcccatccgc gccgtagtcg
     2341 cccgaacggg actcaacccg gacacgattc gggcctggga gcggcgctac ggggcgattg
     2401 agccggaccg aagcgcaggt ggcacccgcc aattcagcga agaagacgtg acccggctgc
     2461 tttgtttgaa agagctccga gagcgcggtt tcagtctgca gaccatcgcc cagctcccca
     2521 cggccgagct caagtccatg cggagcggcg accgggaagc cattccgcgc gtccgtccca
     2581 ctcaaccccc gctatcggat cagcagcagg cgtacttgga tgcgatcgaa aacctcgacg
     2641 ccaacggtgc gctcgagcag ctgcacgcga tgagcgaagg gatcggtaac ctgcagctga
     2701 tcctcggcgt cgccatcccg atcatgtggg aggtcgggga ccggtggagc cagggcactc
     2761 tgggcacagc gccggagcat ctggtgagag cacagctcaa gagcctcatg accgcccgct
     2821 tcgaacaaat cccaaacccg gtgggcgctc ggaagctcct gtttacgacg ccggccggtc
     2881 aattacacga gttcggcatt ctcgctggtt cggtgctcgc caaagaagct ggcttcgacg
     2941 tgctctacct gggccccaat cttccgaccg aggacatcat ttggagcgtt gccaaaagcc
     3001 aagcagacat cctcgtcctg tccgtggtcc ggcgcatgga caatgaggag atcaggcaaa
     3061 tggagcgcct cgtagacacg atcgcgccta ccgtcccggt ttggattgga tgtcctcccg
     3121 aacaccccct catcgcgcgg actccaaagg cgacgttcat cagctcgatc gaggatttcc
     3181 agctggccgc atcctccctg ctctcacgcg cgcccaacta atccgcgaca tcccgagggt
     3241 ttggcgttcg ccccggatgc acgtaggcac atgacagcag ccatcggaat atgtatcgtt
     3301 cgaccgcatg ccgtgcgtga ccgtctcacg aagatggaag agacatcatt gggcgtacgt
     3361 cgcctgtggc ctctggatcc taggcttcga cgtcgttccg ctggcccatg aggtttttca
     3421 cgactcgctc gatgcccacg atcacggcca tcaccatgat tcgcagcatg gtcataggca
     3481 tgagggtgac cccgaga
//
LOCUS       DASRZF010000406         2919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_652199, whole genome shotgun sequence.
ACCESSION   DASRZF010000406 DASRZF010000000
VERSION     DASRZF010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652199"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1817
                     /locus_tag="VFG22_18025"
     CDS             <1..1817
                     /locus_tag="VFG22_18025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418200.1"
                     /translation="PVISSVSTFGSILRVIFNAPPNPEAGVYDVVIQVCNACGSWDIP
                     IRLEVVDPVIIPTTDCGLSQATFPPSATPMDVGDQILAFRAGACITVDPVLPPDVCAT
                     LGAFPEFPLAPVTDRVVVVDGVNNCKLVPADDFLQGLTLCAQIENLITDDIEAGDQVV
                     AFRPGAPNVCVRGSGFDMVCSALQALAPLAGSPTTLVVGTDGGICGTLDLATLYGLLN
                     IPPAFDGGTIANPIEGPDGSCAAPTYSFTTDPTSGLLYEAGVPAVTLGYSNCSAFVRV
                     GDVVRIEASNSDFIEVGASVIADSENGFVTLRGGSAAPGANCGLIVETNAIERLRIGS
                     QGAWNLNAGGGGNLGDIITSQGPGLPPAWAPPAPVALACPVYLPCDGSYAGPSYSWGS
                     RPEAGFLMLDSSIAAPKIGVALEGIRFARMAIDDFNFDQYRRTFQLFGEQTLSTNVFP
                     RWHVTQFYNGHVNHPSIWMHLHSENAIGTGMEASSAALGNNAGYFAFNNDPALGVNGS
                     RNELNTSPGAIGLYIGNSPIANPNRIFVRLEDRVGPITLFQIARDETLNELNITINEG
                     FDGAAAAAGTLTNAPAAGDPTFWLRVTINGVQRFIPAW"
     gene            1821..2075
                     /locus_tag="VFG22_18030"
     CDS             1821..2075
                     /locus_tag="VFG22_18030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418201.1"
                     /translation="MDPKKLNPCEYEIYCKLVEWAETDCWCCTATRGLLVGLVAGITL
                     GLLLGGHPVGAVWFLIVSAPLTIAALVWARKAWADEGEEK"
     gene            2072..>2919
                     /locus_tag="VFG22_18035"
     CDS             2072..>2919
                     /locus_tag="VFG22_18035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418202.1"
                     /translation="MSQKITLFDVNSTSPTSAIINVGEKSCVQIRAFNLGAGEEVLVE
                     LVSLQAGSYPKGVINSCLPNPVASGPQVIAVTPYTPCGYQQFLLDETRNTIVIRSAGF
                     YQFQLSLAAVGTAIVEAIFYEGAEACDAIASECCCQPEIAWLGTSTNPCLTITPGGPA
                     GHSPVFGLDACCLLQSLPANPNPVLTDRLVFLSGPDCFTATIDEVFSMAVVCDSLGEF
                     GIGAYAAGDTLVALDAGENCKRLDIQAAVTATETPWTGTSNNPNLTITPGGVNGHAPI
                     FDYDLCA"
BASE COUNT          498 a          936 c          908 g          577 t
ORIGIN      
        1 tgccggtgat ctcgtcggtg tcgaccttcg gctctatcct gcgtgtgatc ttcaacgcgc
       61 caccgaaccc ggaggctggc gtctacgatg tcgtgattca ggtgtgcaac gcctgcggct
      121 cgtgggacat cccgatccgg ctcgaagtcg tcgacccggt catcatcccg accacagact
      181 gtgggctctc tcaggcgact ttccctccgt ctgcgacgcc gatggatgtc ggcgatcaga
      241 tcctcgcttt ccgcgccggg gcgtgtatca cggtcgatcc ggtcttgcct ccggacgtct
      301 gtgccaccct cggcgccttt ccagagtttc cgctcgcgcc agtcaccgat cgggtagtcg
      361 tcgtcgatgg ggtcaacaac tgcaagctcg tccccgctga cgacttcctc caaggtctca
      421 cgctctgcgc gcagatcgag aacctgatca ccgatgacat cgaggccggt gatcaggtcg
      481 tggcgttccg ccccggggcg ccgaacgtct gcgttcgcgg aagcggtttc gacatggtgt
      541 gctcggcgct acaggcgctc gcgccgctcg ccgggtctcc cacgacgctc gtggtcggaa
      601 cggacggcgg tatctgcggg acgctcgatc tcgccacgct ctacggactt ctgaacatcc
      661 cgccagcgtt tgacggtggg actatcgcga acccgatcga gggtccggat ggctcctgcg
      721 ccgccccgac ctactcgttc acgaccgacc cgacgagcgg ccttctctac gaggcgggtg
      781 tcccggcggt cacgctcggc tacagcaact gctctgcctt cgtgcgcgtc ggtgacgtgg
      841 tgcgtatcga ggcgtcaaac tctgatttca tcgaggttgg ggcgagcgtc atcgctgact
      901 cagagaatgg cttcgtcacg ctgcgaggcg gatctgcggc tccgggggcc aactgtggtc
      961 tgatcgtcga gacaaacgct atcgagcggc ttcgcatcgg atctcaaggc gcgtggaacc
     1021 tgaatgctgg tggtggcggg aatctgggtg acatcatcac gtcgcagggt ccggggctgc
     1081 ctcctgcgtg ggcgccgccc gcgccagtgg cccttgcgtg cccggtgtat ctgccgtgcg
     1141 acggaagcta tgccggaccg tcctactcgt ggggatctcg cccggaggcc ggcttcctca
     1201 tgctcgattc gtcaatagcc gcgccaaaga tcggcgtcgc gctggagggt atccgattcg
     1261 cgcgcatggc gatcgacgac ttcaacttcg atcagtaccg gcgcacgttc cagcttttcg
     1321 gagagcagac cctctcgacg aacgtgttcc cgcgctggca cgtcacgcag ttctacaacg
     1381 gccacgtcaa tcacccgtcg atctggatgc accttcattc ggagaatgcg atcgggacag
     1441 gcatggaggc gtcgagcgca gcgctcggta acaacgctgg ttactttgcg ttcaacaatg
     1501 acccggcgct tggtgtgaac gggtctcgca acgaactgaa cacgagcccc ggggcaatcg
     1561 gactctacat cggtaacagc ccgatcgcga atcccaaccg tatattcgtt cgcttggagg
     1621 accgggtcgg cccaatcacg ctgttccaga tcgcgcgaga cgagactctg aacgaactca
     1681 acatcacgat caacgaggga ttcgacggtg cggcagcggc ggcgggtacg ctcacgaacg
     1741 cccctgccgc cggagacccg acgttctggc tgcgcgtgac aatcaacgga gtgcagcgct
     1801 tcattcctgc gtggtgagac atggacccga aaaagctgaa cccctgcgaa tacgagatct
     1861 attgcaagct ggtcgagtgg gccgagaccg actgctggtg ctgcacggcc acgagaggct
     1921 tgctcgtggg tctcgtggcc ggcatcacgc tcggcctcct gcttggtggt cacccggtcg
     1981 gagcggtgtg gttcctgatc gtttctgcgc cgctcaccat cgctgcgctc gtgtgggctc
     2041 gcaaggcgtg ggctgacgaa ggggaagaga aatgagccag aagatcaccc tgttcgacgt
     2101 caactcgacg tcgccaacgt cggcgatcat caacgtaggc gagaagtcct gcgttcagat
     2161 ccgcgcgttc aacctcggtg ctggcgagga agtcctcgtc gaactggtga gccttcaggc
     2221 tggaagctac ccgaaaggcg tcatcaactc gtgcctgccg aacccggttg catcaggccc
     2281 gcaggtcatc gctgtcacgc cctacacgcc ctgcggctat cagcaattcc tgctcgacga
     2341 gacgcggaac acgatcgtca tccgttcggc cggcttctac cagttccagc tttctctggc
     2401 tgcggtggga accgcaatcg tcgaagcgat cttctacgag ggcgccgagg cgtgcgatgc
     2461 gatcgcatcc gagtgctgct gccagccgga gatcgcgtgg ctcgggacgt ccacgaaccc
     2521 gtgcctgacg atcactccgg gcggtcctgc ggggcattct ccggtgttcg gtctggatgc
     2581 gtgctgcctg cttcaatcgc ttccggcgaa cccgaatcca gtcctcacag atcgcctcgt
     2641 atttctgtcc ggcccggact gcttcaccgc gacgatcgac gaagtgttct cgatggcggt
     2701 cgtgtgcgac tcgctcggtg agttcggcat cggcgcctat gccgcaggcg acacgctcgt
     2761 tgcgctcgac gctggcgaga actgcaagcg tctcgacatt caagcggcgg tcacggcgac
     2821 cgaaacgcca tggaccggaa cctcgaacaa tccgaatctg acgatcacgc ccggaggggt
     2881 gaacggtcac gcgccgatct tcgactacga tctgtgcgc
//
LOCUS       DASRZF010000407         3336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2719178, whole genome shotgun sequence.
ACCESSION   DASRZF010000407 DASRZF010000000
VERSION     DASRZF010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2719178"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..1791)
                     /locus_tag="VFG22_18040"
     CDS             complement(76..1791)
                     /locus_tag="VFG22_18040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418203.1"
                     /translation="MGGLRLGALMVFLSVASSAGVASAEGSHAALLPFQGPQASKVRQ
                     SVQKGLSAKDVLLVPLKDVTAVVKKTKGYAKQAAKLEASALVRTRLRRVEGRWIADTE
                     VWNAEGRRVDKLRTTSSSLARLSNRVVGQLLETGRMPQPGAVVAKSSEREIAAPTKPR
                     IVIRPFSGVQSAKIRGAAVRGLRKESVELFPNDKFVAKARSMGVDLDAEGGHVAPASA
                     LAVSGLIEGDVLHEDGVWSAYVRLIEGDSRKVVSQHFYDANTSAVLAKVVQTNVGSDF
                     KRDIQKLGTAEPVAVVVAPVAAASAIPVEKAPSAKAAVEVEPRATRSKKQRPAAVDIE
                     VDFRMVNRRLSYADDLNGDLRDYQLKFGPGVGTKFQYYPGAHFTAGVGAQFGIDFEWQ
                     RIFDFESTRADGAQFPTQSQQFLIGMRWRYPKGRWEPSVALDYGVHTFEFGVSGPPQP
                     GEDTTSGVPGVRYEFVRFGGGFRVVIGKKEILIITTSIAYRAVFSVGSIGTYLWFPEA
                     TANGMDAMLMLGFALPKGFEVRLGVDYRRYGFDLNPVPPDPPYVAGGALDQYWGLSIG
                     LAWRR"
     gene            complement(1795..2241)
                     /locus_tag="VFG22_18045"
     CDS             complement(1795..2241)
                     /locus_tag="VFG22_18045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418204.1"
                     /translation="MKRFALYTFGVGLISLFVGCVGSLANPGDFLDGGTTQKSTEAVL
                     AESCGTVGCHDASSRAQAGLDLVSPNVASRVVDVNATGLGCGNDVLVVAGDPDNSYLL
                     DKVLDELGICGLPMPVVGSLSASEVEVLRQWIIDLGGSGGGTPDGG"
     gene            complement(2336..3220)
                     /locus_tag="VFG22_18050"
     CDS             complement(2336..3220)
                     /locus_tag="VFG22_18050"
                     /inference="COORDINATES: protein motif:HMM:NF016658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HET6418205.1"
                     /translation="MIDGRRPWFGRWWAVVLLIALVVLATAMLTVKALGLRSVEARAE
                     PFEQPLDDFDWGWWNQALGRWVRAAGVDYDAVRSDEGDLRRFVATLGAVGPRSTPERF
                     GTEPERLAYYINAYNTLVLFAVVDNWPIHTVHDVRGWLDPRAGFGFFYGLRFPLDGDS
                     TNLYDLENEVIRGFLDARIHAAINCASKSCPALMPYAFEAASLDEQLDSVMRGFCSSA
                     KHVRVDEEAREIQLSAIFDWYRSDFEEHARRLGRPATIVDFILAFATPEVAAELERSR
                     GDGFDVVFQAYDWDLNEI"
BASE COUNT          656 a         1123 c          955 g          602 t
ORIGIN      
        1 gtggtccacg cccggcacat gctcgaagcg taacctcgaa cggcagcgag gggtacttcg
       61 tgcggccgga ccgttctatc gacgccaggc caggccaatg gataagcccc agtactggtc
      121 cagcgcccca ccggcgacgt agggcgggtc gggcggaact ggattgaggt cgaagccgta
      181 gcggcgatag tccacgccga gtcggacctc gaagcccttc ggaagcgcga atccaagcat
      241 cagcatggca tccatgccgt tggcggtcgc ttcgggaaac cagaggtatg tgccgatcga
      301 gccaaccgag aacaccgcgc ggtacgcgat gctcgtcgtg atgatcaaga tctctttctt
      361 accgatcacc acgcggaagc caccaccgaa tcggacgaac tcgtatctca cgcccgggac
      421 gcccgaggta gtgtcctccc ccggctgcgg cggtccggag acgccgaact caaaagtgtg
      481 cacgccatag tccagcgcca ctgagggctc ccaccgccct ttcgggtagc gccatctcat
      541 gccgatcagg aactgctgag actgggtcgg gaactgtgcg ccgtccgcac gcgtcgactc
      601 gaagtcgaag atccgctgcc actcgaagtc gatgccgaac tgcgcgccaa cgcccgcagt
      661 gaaatgcgcg ccggggtagt actggaactt cgtgccgacg cccggaccga atttgagctg
      721 gtagtcgcga aggtcaccgt tcagatcgtc ggcataactc aagcgacggt tgaccatgcg
      781 aaagtcgacc tcgatgtcca ccgcagccgg tcgttgcttt ttggaccgcg tcgccctggg
      841 ctcgacctcg acggcagcct tggcgctggg cgccttctcg acggggatcg ccgatgccgc
      901 ggcgaccggc gccacgacga cggctacggg ctcggcggtg ccgagcttct ggatgtctct
      961 tttaaaatcc gatccgacgt tggtttgcac aactttcgcg agcactgccg atgtgttcgc
     1021 gtcgtagaag tgctggctga ccactttccg gctatcgcct tcaatcaggc ggacatacgc
     1081 cgaccagacc ccgtcttcgt gcaggacgtc gccctcgatg agaccactga ccgcaagggc
     1141 gcttgcggga gcgacgtgcc cgccctcagc gtcgagatcg acgcccatgc ttcgcgcttt
     1201 ggccacgaac ttgtcgtttg gaaagagctc gaccgactcc ttgcgcagcc ccctcaccgc
     1261 tgcgcctcga atcttggcgg actggacgcc tgaaaacggc cggatgacga ttcgcggctt
     1321 cgtcggtgcc gcgatctccc gctcgctgct tttcgccaca acggctcccg gctgcggcat
     1381 ccgtcccgtc tccagaagct gacccactac gcgattcgag aggcgcgcca ggctggacga
     1441 agtggtacga agcttgtcca cgcgtcgtcc ctcggcattc cagacttcgg tgtcggcgat
     1501 ccaacggccc tcaacccggc gaaggcgtgt tcgaaccagc gcgcttgctt cgagcttggc
     1561 cgcttgcttc gcgtaacctt tcgtcttctt gacgacggca gtcacatctt tcagcggaac
     1621 caagagcacg tccttcgcgc tcagaccctt ttgaacgctc tggcggacct tggacgcttg
     1681 cggcccttgg aatggcaaca gtgcggcgtg gctgccctcg gccgacgcaa cgcccgccga
     1741 cgaagccacg ctcagaaaca ccatcagcgc gccgagacgc aaccctccca tcgactaacc
     1801 tccgtccgga gttccgcccc cggaaccgcc taaatcgatg atccactgtc ggagcacttc
     1861 cacctcgctc gctgagaggc tgcctacgac cggcatcggg aggccgcaga tacccagctc
     1921 atcgaggacc ttgtcgagca ggtagctgtt gtccgggtcc cccgcgacga cgagaacgtc
     1981 gttgccgcac cccaatccag tcgcattgac gtcgactacc cgactcgcga cgttcggcga
     2041 aaccaaatcg agccccgcct gtgctcgcga ggacgcgtcg tggcagccca ccgttccgca
     2101 gctttcagcg agcacggctt cggtgctctt ttgcgtcgtt ccgccgtcca agaaatcgcc
     2161 gggattcgcc aaactcccca cgcagcccac aaacaacgaa atcaagccga ccccgaacgt
     2221 gtacaaagca aagcgtttca ttgcgcgcgt tgatcccccc gaatcatccc taaagcttag
     2281 taagctatgg cacccttcgt cgagttatgc agtatttttg tgcaacgaat cgttcctaaa
     2341 tctcgttgag atcccagtcg taggcctgaa aaacaacgtc aaaaccgtcg ccgcgcgatc
     2401 gctcgagctc tgcggccacc tcaggcgtgg caaacgccaa gatgaagtcg actatcgtgg
     2461 cgggccgacc tagacggcgg gcgtgctcct cgaagtcact gcggtaccaa tcgaagatcg
     2521 cggacagttg gatctcccgg gcctcctcgt ccacgcgtac gtgcttagcg ctcgagcaaa
     2581 agccacgcat gacggagtcc agttgctcgt cgaggctggc cgcttcgaat gcatacggca
     2641 tcagcgccgg acatgatttc gaggcgcagt tgatcgccgc atgaatccgc gcgtcgagga
     2701 acccgcgaat cacttcgttc tcgagatcgt agaggttcgt cgaatcgcca tcgagcggaa
     2761 agcgaagccc gtagaagaac ccgaatcctg cgcgcgggtc caaccagcct cggacgtcgt
     2821 gcaccgtgtg gatcggccag ttgtcgacga ccgcgaaaag cacgagcgtg ttgtacgcgt
     2881 tgatatagta agcgaggcgt tcaggctccg taccaaagcg ctcaggcgtc gaccggggcc
     2941 ccaccgcgcc cagcgtggca acgaaccggc gcagatctcc ctcgtcgctg cgcaccgcat
     3001 cgtagtccac gccagctgcg cgcacccatc ggccgagcgc ttggttccac cagccccaat
     3061 cgaagtcatc cagaggctgc tcgaacggct ccgcacgtgc ctcgacggaa cgcaaaccga
     3121 gcgccttcac cgtgagcata gctgttgcca gcacgaccag ggcgatcaag aggacgaccg
     3181 cccaccatcg tccaaaccaa ggtcttcgac catcaatcac gtcgggagta ttgcacggcg
     3241 ctccgattcg ttacatcccg ctcatcaacg acatgtcggg catcacgatt tggcacaatc
     3301 ctcgctgcag caagtctcgt cagactttgc agctca
//
LOCUS       DASRZF010000408        10604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_653843, whole genome shotgun sequence.
ACCESSION   DASRZF010000408 DASRZF010000000
VERSION     DASRZF010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 10604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10604
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653843"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="VFG22_18055"
     CDS             <1..1080
                     /locus_tag="VFG22_18055"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HET6418206.1"
                     /translation="EGIGYVQVNMKGGQRWSSADAFLSPEVKKRPNLKIVLKVLAKRV
                     LLDGKKAVGIEYQDAKGNTHQARANREVVLSAGTYNSPPLLELSGIGKPEVLRELGVE
                     VKHALPGVGENLQDHLQLWVQQGVKTKECLSEDGKFPRVVGAVLKYLFTKKGPLTMPA
                     CNVGGFVPNEGAERPIFQIHFTPGAGGMDENGKMVATPDPGVNSTVCIIRPTSRGSVH
                     ARSTDPKAFPKILHNYMSTEHDRSLAIEGFRLQRKIYQSEAFKPYATYEILPGESVQT
                     DEEIMEFWRREGMSVYHPVGSSKMGPAEDPNAVVDNELRVHGIQCLRIVDASIFPLLP
                     SGNTHAPTVAVAERAADLILGNSVA"
     gene            1125..1198
                     /locus_tag="VFG22_18060"
     tRNA            1125..1198
                     /locus_tag="VFG22_18060"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1159..1161,aa:Arg,seq:tct)
     gene            1522..2037
                     /locus_tag="VFG22_18065"
     CDS             1522..2037
                     /locus_tag="VFG22_18065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418207.1"
                     /translation="MRYLFGLICVLALSVMPLAGCSDDEGVGGSGGSAGTGGSGGGTA
                     GAGGMAGTGGSEPSPVGLWTGSGQDAPDGPLTICFNVREDGGALVASLDTNQECQGWS
                     LWVKFENCEGQFATNEEIPIVDGAFNLLLESLSSNISGTFDGNTASGEATTLAVGGGT
                     CAGTWQASPSP"
     gene            complement(2081..4315)
                     /locus_tag="VFG22_18070"
     CDS             complement(2081..4315)
                     /locus_tag="VFG22_18070"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET6418208.1"
                     /translation="MPKASKGGTIRHLGPLQATGVGVGAIVGGGILVLAGAAFKATGP
                     SAILAFTLNGIVAVITALSFAEMSSMFPESGGAYTFAKKVLTVRAAFVVGWILWFAYI
                     VAGVLYALGFAEYALLAIAELFDAVHGTEPDWLRTRAAAIALAVIATSAYGLLLIRKS
                     SGGGQWETIGKMVVFAVLILAGIWALAVSPSGTVERGMTPFFENGTSGLLTAMGFTFI
                     ALQGFDLIATVGGEVKDPERTIPRAMLTSLGIGLAVYIPLLFLISTVGTVPGHGIATM
                     SAERPATVMADAAYNFSGPIGYWLVIIAAVLSTLSALSANVLAASRIALQMASDRTLP
                     RVLQQRHSTRGTPVMAIYATALAMAIILLMVPSVSSAGAAASLIFLISFALVHWTNLL
                     ARLRSPVEAPFRTPWFPAVPVIGGLACAILALYQAVAVPAAGAIAVVWLGLGVILYFG
                     LFAGRAQAVDAFAEARDSNLGALRGRSPLVLVPVANPSSAVGMVTLANALAIPVVGRV
                     LLLTVVRRPSDFATAEQATRQALADAQEVVGQAMSASLAAGDAPEALTTIARDPWKEI
                     VRIADTRSCDSLLVGFSSLEGDGNVERIEHLLNEVDCDVVALRAPKDWSFNSETRIIV
                     PVGGRGGHDVLRARLLGSLGRAGLRNVCFVRATSERLRPATKAHLERALRIFAEEETY
                     DVPQTKVIECDDIVEALAGEAGPQDLIIVGLRHQRGKRLFSELALQVARRTPAATLMI
                     SRRY"
     gene            complement(4377..6713)
                     /locus_tag="VFG22_18075"
     CDS             complement(4377..6713)
                     /locus_tag="VFG22_18075"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET6418209.1"
                     /translation="MVPGLTEAQAERRQVQVTAVYDGPSPVFGAAIDDLSKEIQAQLS
                     DRFDVVFVPLSSVGDWTSAGVERQLNEAYANPGISAVFGFGHLTVHAVATRKNLPTPT
                     ILPFVTEGRKQGLPRNGDVSGKRNLTYITEDLDVSEEANWLRKVAGSKHIVLLAEESL
                     REILPAATGDRSISIAIAEPSVEALLTAIPSAADGVILTPMRQLSLEDRVRLLEGITE
                     RRLPNIAVGPTWLQQGAMMSIRSPNNAARRTQRAALDLDLILEGRPASQIHIDFEERM
                     ALQFNLDAARAVGVRPTYEVLLEADTMQQEEADDENQVDLGIAMLEAADQNLELLVSE
                     KFVEAGEEGVKRERGPLLPQGQLQVGATYRDPDRIAPGGQIAEWQGSTVARASQSIYS
                     AEAWARFRSRQLRQEGREYGYFSDVLDIMLQAGLDYVAVLRAEAQERIQRENIQLTRE
                     YLELARLRLEIGVANASELYRWQVTLAENQRSVVEARALVGQTKVELNRVLNRPLERP
                     IAPVDLPVDEAGRAAPPDDPIGKYMLDPWSFERLRDFMVREGLRNSPTVREIEAREAA
                     QERIREGRTLELWLPEFAVEGGVQHDFWRAGEGATTPPDLEAFGIPQPDKFGWDVGLY
                     LAIPLSRGGAGIANIRESERLVERLSAELARIEQNVDTGVRTELYNASAAMASVSLTR
                     KAAVAAADNLVLVTDLYRRGKVDIIRLVDAQTQSLVADLAAVNAVYDYLIALLFVNRE
                     ISHFRNLDTDEAKQEFSRRLNEFILASESASVTSAPRP"
     gene            6920..8029
                     /locus_tag="VFG22_18080"
     CDS             6920..8029
                     /locus_tag="VFG22_18080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET6418210.1"
                     /translation="MTPNNPSATLRLVPLLLVLAMLAACKKEELAAVEPPPRPIKIYR
                     LDGQLGATRPDYPGRIKAIKVVELAFEVEGRIIDWSVEEGQRVRKGQILSRLDARDYQ
                     AQVEASLARLRLADAEYRRESKLFASGSGTQRDLDVATRSRDVSRADLRISKKALEDT
                     KLRAIFDGVIARKLVSDFRNVAAREPVLLLQDESIMEVVVDVPERDLTARRDEVDLAE
                     WNDKAKPMVELSALPGRQFPVRLAELATAADPETRTFRATFNMKKPPDVGVLAGMTAK
                     LVLTGLRTTASDDFLLPVNAVAADSNDEPYVWLVDQNSMTVSKRPVEAGRVTGDRIVI
                     KSGLEGGEAVAMSGVHLLAEGMQVTEMAESDETRK"
     gene            8026..>10604
                     /locus_tag="VFG22_18085"
     CDS             8026..>10604
                     /locus_tag="VFG22_18085"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET6418211.1"
                     /translation="MNLTDFALRNRTLIVVLTIVSVFAGMKTFFGLPRLEDPEFTIKE
                     ALVVTPYPGATAFEVEQEVSDELELAIQKLGKIKKIETRNTPGKSNISVEMRSTVPGK
                     VLPQVWDELRRKVGDAQSKLPPGAGPSLVIDDYSDVYGVFFALHGDEYSYRELSDVAK
                     MLRKELILVKDVAKIELFGDVPEAIYVEFDRERLSQIGISTGDIGQQLVAEGIVVDAG
                     RVQAGPEIIKIQPDSLIHSSQDLGDILITKGDRTQVRLRDIANITRGYKEPATAKLHM
                     DGHRAIGIGVSTEQGGNVVNMGEAIKLRLAELDEQIPLGMELEVIYMQSDMVTTAIDA
                     FLVNLAEAVAIVIVVLLFFMGVRSGLIIGFVLVLTIAMTFIFMGPAGLALERISLGAL
                     IIALGMLVDNAIVIIDGMLVRINGGMDAEEAAKEVAGQTSMPLLGATAVAIIAFAAIG
                     LSPDSTGEFCSSLFTVIGISLSLSWVTAMTITPLLGIWFIKPPKPGTTGESDEAYGGG
                     FYGGYKKFLGLCLRFRWVTVAVVLGLFALSVYGFQFTDKAFFPASTTPKLRVDVFWPI
                     DQHIDETERKATVIERGLAELEGVTHVATSVGQGPLRFVLTFTPEKEHSGYAQFLVDI
                     EDYHTLAADIEKAEAYIQEVAPDAIAFGRTFDLGPSKPGKIEARLVGHDPNVLRALED
                     QVLEIFKADPDTKGSRGRWYERVKVIRPGLSEEQADAQGITTGMVADAIRSTFEGKQE
                     GVYREGDEVIPVVFRQPAPTRLDVGNLRQIPIWSPMAQRYVPLANVVTELETEWTDQV
                     VERRNRKRTLTVVTDPTEDSAPTLWNRLAPQVEALSFPPGYYVEWGGEYESQQDAQAA
                     LFAP"
BASE COUNT         2034 a         3238 c         3413 g         1919 t
ORIGIN      
        1 gagggcatcg gctacgtgca ggtgaacatg aagggcggcc agcgctggag ctcggcggac
       61 gcctttttga gccccgaggt caagaagcgg ccgaacctga agatcgtact caaggtgctg
      121 gcgaagcgcg ttcttctcga cgggaagaaa gccgttggga tcgagtatca ggatgcaaaa
      181 ggaaacacgc atcaggccag ggcgaatcgc gaggtcgtgc tctcggctgg gacttacaac
      241 tcgccgccac tgctcgagct gtcgggaatc gggaagcccg aggtgcttcg agagctcggc
      301 gtcgaggtga agcacgccct ccccggcgtt ggggagaact tgcaggatca tcttcagctt
      361 tgggttcaac agggcgtcaa aacgaaggag tgcctgtccg aggacgggaa gttcccgcgc
      421 gtggtcgggg ccgtgctcaa gtacctcttc accaagaagg ggccgctcac gatgccggcc
      481 tgcaacgtgg gcggtttcgt cccgaacgag ggcgccgaac gaccgatctt tcagatccac
      541 ttcacgccgg gtgcgggcgg catggacgag aacggcaaga tggtcgcaac cccggacccc
      601 ggtgttaatt ccaccgtgtg catcattcgc ccgacctcgc gcggcagcgt ccacgcgcgc
      661 agcacagacc cgaaagcgtt cccgaagatc ctccacaact acatgtcgac cgagcacgac
      721 cggagcttgg cgatcgaagg cttcaggcta cagcgcaaga tctaccagtc cgaggcgttc
      781 aagccgtatg cgacctacga aattcttccc ggcgagtcgg tgcagaccga cgaagagatc
      841 atggagttct ggcgaagaga aggcatgagt gtctatcacc cggtcggctc gtcgaagatg
      901 ggcccagccg aggatccgaa cgcggtcgtc gacaacgagc tccgagtgca cgggattcaa
      961 tgtctgcgta tcgtcgacgc gtcgatcttc ccgcttctgc cgtcgggcaa cacgcacgcg
     1021 ccgacggtcg ccgttgcaga gcgggcggcg gatttgattt tggggaattc agtggcgtag
     1081 cgagcgggac gcgaaatgcg tatcgtgcta gacagccgcg aaacgcgcct gtagctcagc
     1141 tggatagagc ggcggccttc taagccgatg gtcgggggtt cgagtccctc caggcgcgcc
     1201 gatttacaag ggattctgac aacgtcgaga tcaagctgtg cgaatgcacc tgcgagcact
     1261 gctatgagaa gtatgagtgg cgcgccagcc gatctcgaga cagcccgagc ccatatcggt
     1321 accttgcgtg ctggctaacg cggccctact tgcagctagc cctcgcagcc gtagccacgc
     1381 actcgttgcg ccgccacgcc cccgaaacgc aagatttgct agtcaccgtt gccgaaattt
     1441 ctgcgaaaaa cgggtctggc atagctgatg catccgcatg atccgacgga gttacgtcct
     1501 gcgactggat cggaggttgc tatgcggtac ttgttcggat tgatttgcgt gctcgcgctg
     1561 agcgtcatgc cgctggctgg ttgcagcgac gatgagggcg tgggcggtag tggtggctcg
     1621 gctggaaccg gcggtagcgg tggcggaact gcaggcgccg gcggcatggc tggtaccggc
     1681 ggtagcgaac catctcccgt aggcctctgg acaggctccg gccaggacgc tcccgacggt
     1741 cctctcacca tctgtttcaa cgtgcgagag gacggaggtg cgctcgtggc atccctggat
     1801 acaaatcagg aatgccaagg ctggtccctg tgggtcaagt ttgagaactg tgagggccag
     1861 ttcgccacaa acgaggagat ccccattgtc gacggggcct tcaatctgct cctcgagagc
     1921 ttgtcctcca atatcagcgg caccttcgac ggcaacaccg cttctggtga ggcgaccacc
     1981 cttgccgtgg gtggcgggac ctgcgcaggg acatggcaag ccagccctag cccgtgacaa
     2041 cagcttctac tgcaccgacg gcacgcgggc agccgcgcac tcagtaacgc cggctgatca
     2101 tcagcgtcgc agctggggtc ctcctcgcga cctgcaacgc caactcgctg aagagtcgct
     2161 tgccgcgctg gtgtcggagg ccgacgatga tgagatcctg gggaccggct tcgcccgcca
     2221 gcgcttcgac gatgtcatcg cactcgatga ccttcgtctg cggaacatcg taggtctctt
     2281 cttcggcgaa gattcggagc gcgcgctcca aatgggcctt ggtcgccggt cgcagccgtt
     2341 cgctcgtggc ccggacgaag cacacgtttc taagacccgc gcggcccagg cttccgagca
     2401 ggcgtgcgcg cagcacgtcg tgtccgccgc gtcctccgac gggaacgatg attctcgttt
     2461 cgctgttgaa cgaccagtcc ttgggcgcgc gtagcgcgac cacgtcgcag tcgacttcat
     2521 tcaagaggtg ctcgatgcgc tcgacgttac cgtcgccctc gagtgacgag aaccctacga
     2581 gcaggctgtc gcaggaacgg gtgtcggcga tgcgcacgat ttctttccat gggtcgcgcg
     2641 cgatcgtggt gagggcctcg ggtgcgtctc ctgcggcgag cgatgcggac atcgcctggc
     2701 cgacgacttc ctgggcgtcc gccagggctt ggcgggtcgc ttgttcggca gtggcgaagt
     2761 cgctcgggcg gcgcacgacg gtgagcaaga ggacccggcc gacgactggg atggcgaggg
     2821 cgttggccag tgtgaccatg cccacggcgc tcgaggggtt ggcgaccggt accaaaacga
     2881 gcgggcttcg gccgcgaagg gcgccgaggt tcgaatctcg cgcttcggca aatgcgtcga
     2941 cggcctgcgc tcggcctgcg aacaaaccga aatagagaat cacacctagg cccaaccaaa
     3001 cgacggcgat ggcgcctgcg gcggggacag cgacggcttg atagagcgcg agtattgcgc
     3061 aggccaggcc gccgatgacc gggaccgcgg gaaaccaggg tgtgcgaaaa ggggcctcga
     3121 cgggtgaacg aagccgcgcg agcaagttgg tccaatgcac gagcgcgaag gagatcaaga
     3181 agatcaggct tgccgcggcg ccagcagaag aaaccgacgg caccatcagc aagatgatcg
     3241 ccatcgccaa tgccgtggcg tagatcgcca tcaccggagt gccccgcgtc gagtgacgct
     3301 gttgcaggac cctcgggagg gtccggtcgc ttgccatctg gagcgcgatg cggcttgcgg
     3361 ccaggacgtt ggcggagaga gctgaaaggg tggagaggac ggccgcgatg atgaccagcc
     3421 agtagccgat cggcccgctg aagttgtacg cggcatcggc catgacggtg gcggggcgct
     3481 ccgcgctcat ggtcgcgatc ccgtgccccg gcacggttcc caccgtggaa atcaggaaga
     3541 gaagagggat gtacacggcc aggccgatgc ctagggaagt cagcatcgcg cgggggatcg
     3601 tccgctccgg gtctttgacc tcgccgccaa ccgtggcgat gaggtcgaag ccctgcagtg
     3661 cgatgaaggt gaagcccatg gcggtcaaca ggcccgaggt tccgttctcg aagaacggtg
     3721 tcatgccgcg ttccaccgtt ccggatggcg acaccgcaag cgcccagatt cctgcgagga
     3781 tcaaaaccgc aaacaccacc atctttccga tggtttccca ttggccaccg cctgagctct
     3841 tgcgaatgag tagcaagccg taggcagatg tggcgatcac ggcgagtgca atagcggcgg
     3901 cgcgggtgcg caaccagtcc ggctcagtcc catgaacggc atcgaaaagc tctgcgatcg
     3961 ccagcagcgc gtactccgcg aagcccaacg catagagaac gccggccacg atgtacgcga
     4021 accataggat ccagccgacc acgaaggcgg cgcggaccgt gagcaccttc tttgcgaacg
     4081 tgtatgcgcc gcccgactcc gggaacatgg acgacatttc cgcgaagctc agcgcggtga
     4141 tcaccgcaac gatgccattg agcgtgaacg cgaggatggc gctcgggccc gtcgctttga
     4201 aggcggctcc ggcgaggacg aggattccgc ccccgacgat cgcgccgacg cccacgcccg
     4261 tcgcctgaag cggacccagg tgtcgaatcg tccctccctt tgaagccttt ggcacggggg
     4321 ggaacatatt cgcttagtgc gtgctcgcca aacgatcggc gccgcgcagg gggtgtctac
     4381 gggcgcggag ccgaggtgac cgaggcggac tccgaggcca ggatgaactc gttcaggcgc
     4441 ctcgagaact cctgctttgc ctcgtccgtg tcgaggtttc ggaagtggct gatttcacgg
     4501 ttcacgaaga ggagagcgat aagataatcg tagaccgcgt ttacggcagc gaggtccgcc
     4561 acgaggctct gggtttgcgc gtcgacgagc cggatgatgt cgaccttgcc gcgacgatag
     4621 aggtcggtga ctagcacgag attatcggct gcggcgaccg ctgctttccg ggtcagggag
     4681 acggacgcca tggcggccga ggcgttgtag agctccgtgc ggactccggt gtcgacgttc
     4741 tgctcgatgc gagcgagctc cgccgagagg cgctccacca agcgttcgct ttcacgaatg
     4801 ttggcgatac ccgccccgcc gcgcgacaga gggatcgcga gatagagccc cacgtcccag
     4861 ccgaatttgt cgggttgagg gattccaaaa gcctccagat caggtggggt cgtggcgcct
     4921 tcgcctgctc gccaaaagtc gtgctggacg cctccttcga cggcgaattc cgggagccag
     4981 agctcgagcg tgcggccttc tctgatgcgt tcttgcgccg cttcgcgcgc ttcgatctcg
     5041 cggaccgttg gcgagttgcg gagaccttct cgaaccatga agtcgcgcag gcgctcaaac
     5101 gaccagggat ccaacatgta cttcccgatc ggatcgtccg gaggagctgc gcggcccgcc
     5161 tcatcgacgg gcagatcgac gggggcgatt gggcgttcga gagggcgatt gaggacccgg
     5221 ttgagctcga ccttggtttg accaacgagt gcccgcgctt cgaccaccga gcgttggttc
     5281 tcagcgaggg tcacttgcca acggtagagc tcgctcgcat tggccacgcc aatttcgagg
     5341 cgcaggcggg cgagctcgag gtattcacgc gtgagctgga tgttttctcg ttgaattcgt
     5401 tcttgcgcct cggcgcgcag cacggcgacg tagtccaaac cggcctgcaa catgatgtcg
     5461 agcacgtcgg agaagtatcc atactcgcgc ccttcttggc ggagctggcg cgatcgaaac
     5521 cgcgcccagg cctccgcgga gtagatgctt tggctcgccc gggcgacagt gcttccctgc
     5581 cattccgcga tctggccacc gggcgcgatg cgatccggat cgcggtacgt ggcgcccacc
     5641 tggagctgac cttgtggaag caagggcccg cgctcgcgct tgacaccttc ctcacctgct
     5701 tcgacgaact tctcggaaac caggagctcc aaattctggt cggccgcttc cagcatggcg
     5761 atgccgaggt cgacctgatt ctcatcgtcg gcctcctctt gctgcatggt gtctgcttcg
     5821 agcaatacct cgtaggtcgg tctcacaccc acggcgcgag cggcgtcgag gttgaactgc
     5881 agcgccatcc gttcttcgaa gtcgatgtgg atctgggatg ccggccggcc ctcgagaatc
     5941 aaatcgagat cgagggccgc ccgctgcgtc cggcgagccg cgttgttggg cgaccggatc
     6001 gacatcatcg cgccctgctg gagccacgtg gggccgaccg cgatgttggg aagacgtcgc
     6061 tcggtgattc cttcgagcag tcgcacacga tcttcgaggg agagctgccg catcggcgtg
     6121 aggatcacac cgtcggcagc gctcggtatc gcagtcagaa gcgcttcgac gctgggctct
     6181 gcaatcgcga tcgagatcga gcgatctcca gtggctgcgg gcagtatctc gcgcagcgat
     6241 tcttccgcaa gtagcacgat gtgcttggaa ccagcgacct tcctcagcca gttggcctcc
     6301 tcgctgacgt cgagatcctc cgtgatgtat gtcaggttcc gcttgccact gacgtcgccg
     6361 tttctaggca ggccctgctt tcgcccttcc gtgacgaagg gtagaatggt gggcgtcgga
     6421 aggttcttcc gcgtcgccac ggcatgaacg gtgaggtgac cgaaaccgaa tacggcgctg
     6481 atcccgggat tggcgtacgc ctcgttcaac tggcgttcga cgccggctga agtccaatcg
     6541 cccaccgagc tcaaggggac gaatacgacg tcgaaacggt ccgaaagctg cgcttgaatc
     6601 tccttcgaca aatcgtcgat cgcggccccg aagaccgggg aagggccgtc gtagacggcg
     6661 gtgacctgaa cctgacgcct ttccgcctgc gcttccgtca gaccggggac catcaataag
     6721 gcaaatatgg aaaccgaaag cagccgcata ggccgccgag catagtcgac ctggccacgg
     6781 atctcaggct ctcttttcaa aattctttcg ccacgcgcac gtcagcagca aaggattgac
     6841 cttggcaatg ctgatcgagg ttcctatgct gccgcgctac gcccagcttg tgatagaagc
     6901 ctagaatatg gagcgctcca tgaccccaaa taatcccagc gcgactctgc ggctggtgcc
     6961 tcttttgcta gtcctggcca tgctggcagc ttgcaagaag gaagagctcg ccgcggtcga
     7021 gccgcctcca agaccgatca agatctaccg actcgacggc cagctcggag ccacgcgccc
     7081 agattacccg ggccgcatca aggccatcaa ggtggtcgag ctcgccttcg aggtcgaggg
     7141 ccgaatcatc gactggagcg tcgaagaggg gcagcgagtc aggaagggtc aaatcctttc
     7201 gcgtctcgac gcgcgagact atcaagctca agtcgaggcg tcgctcgccc ggctgcggct
     7261 cgctgatgcc gagtacaggc gcgagagcaa gctctttgca tcgggctcgg gtacgcagcg
     7321 tgacctcgac gtcgcgacgc gttcacggga cgtcagccga gccgatcttc gcatctcaaa
     7381 aaaagccctc gaggacacca agcttcgagc gatcttcgat ggggtcatcg ctcgcaagct
     7441 cgtttcagat ttcaggaacg tggccgcgcg cgagcccgtg ctcttgctgc aggacgagtc
     7501 gatcatggag gtcgtcgtcg atgtgccgga gcgggatctc acggcgcggc gagacgaggt
     7561 cgatcttgcg gagtggaacg acaaggcaaa gccgatggtc gagctctcgg cgcttcccgg
     7621 gcggcagttt cccgtccggc tcgccgagct tgccaccgcg gccgatcccg agacgcgcac
     7681 gttcagggct acgttcaaca tgaagaagcc tcccgacgtg ggcgtgctcg ccgggatgac
     7741 ggccaagttg gttctcaccg ggctccgcac gaccgcgtcg gatgatttct tgctcccggt
     7801 gaatgcggtg gcggctgaca gcaatgacga gccgtatgtc tggctggtcg accaaaactc
     7861 catgacggtt tcgaagcggc ctgtcgaggc tgggcgcgtc accggagatc ggatcgtcat
     7921 caagagcgga ctcgaaggcg gagaggccgt cgcgatgtcg ggtgtccacc tcctagccga
     7981 gggcatgcaa gtcaccgaaa tggctgaatc ggacgaaact cggaaatgaa tctcaccgac
     8041 ttcgcccttc gaaaccgaac cctcatcgtc gtcttgacga tcgtctccgt gttcgccggc
     8101 atgaagacgt tcttcgggct tccgcgcctg gaggatccgg agttcacaat caaagaggcg
     8161 ctggtcgtga ctccgtatcc cggcgcgacg gcattcgaag tcgagcaaga ggtcagcgac
     8221 gagctcgagc tggcgatcca gaagctaggc aagatcaaga agatcgagac gcgaaacacg
     8281 ccgggcaaat ccaacatcag cgtcgagatg cgctcgacgg tgccgggcaa agtgctcccc
     8341 caagtctggg acgagcttcg ccgaaaagtt ggtgatgcac agtccaagct tccaccgggc
     8401 gccgggccgt cgctggtcat cgatgactac tccgacgtgt acggcgtgtt tttcgcactt
     8461 cacggagatg agtacagcta ccgcgagctc agcgacgtgg cgaagatgct tcgcaaggag
     8521 ctcattctcg tcaaagacgt cgccaagatc gagctcttcg gcgacgtccc cgaagcgatc
     8581 tacgtcgagt tcgaccggga gcgtctttcg cagatcggga tctcgaccgg cgacattggg
     8641 cagcagctcg tggcagaagg catcgtcgtg gacgccggcc gggtccaggc gggacccgag
     8701 atcatcaaga tccagcccga ctcgctgatc cattcctcgc aggatctcgg cgatattttg
     8761 atcaccaagg gcgaccgcac ccaagtgcgg ctgcgcgaca tcgcgaacat cactcggggc
     8821 tacaaggagc cggcgacggc caagctccac atggacggtc acagggcgat cgggatcggt
     8881 gtgtcgaccg aacaaggcgg caacgtcgtc aacatgggtg aagcgatcaa gctccgcctg
     8941 gcggagctcg atgagcagat ccccctcggg atggagctcg aggtcatcta catgcagtcc
     9001 gacatggtga ccactgcaat cgatgccttc ctggtcaatc tcgccgaggc ggtcgccatc
     9061 gtgatcgtcg ttctcctgtt cttcatggga gtgcgcagcg ggctcatcat cggcttcgtt
     9121 ttggtcctca cgatcgccat gaccttcatc ttcatggggc cggcaggcct tgccctcgaa
     9181 cggatttcgc tcggcgcgct catcatcgcg ctcggtatgc tcgtggacaa cgcgatcgtg
     9241 atcatcgatg gcatgttggt ccgcatcaat ggtggaatgg acgccgaaga ggcggccaag
     9301 gaggtggccg gccagacctc gatgccgcta ctgggcgcaa cggcagtcgc catcatcgct
     9361 ttcgcggcga tcgggctctc acccgatagc accggagaat tctgtagctc tctcttcacc
     9421 gtcatcggca tctctctctc gctcagctgg gtgaccgcca tgacgatcac gccgctgctc
     9481 ggtatctggt tcatcaagcc acccaaaccg ggcacgacgg gcgagagcga cgaggcctac
     9541 ggtggaggct tttacggggg ttacaagaag ttcctggggc tttgccttcg ctttcgctgg
     9601 gtcacggtcg ccgtcgtcct cggtttgttt gcgctttcgg tctacggttt tcagttcacc
     9661 gacaaggcgt tctttccggc gtcgacgacg ccgaagctcc gcgtggacgt cttctggccc
     9721 atcgaccagc acatcgacga gaccgagcgc aaggccacag tcatcgaacg aggattggcg
     9781 gagctcgaag gtgtcacgca cgtcgcgacc tcggtcggcc aaggtccgct tcgcttcgtc
     9841 ttgacgttca cccctgagaa agagcacagc gggtacgcgc agtttttggt cgacatcgag
     9901 gactatcaca ccctcgcggc cgacatcgaa aaggcagagg cttacatcca ggaggtcgct
     9961 ccggatgcca ttgcatttgg ccggaccttc gacctcggcc cgagcaagcc aggcaagatc
    10021 gaggcgcggc tcgtcggtca cgatcccaat gtcttgcgtg cgctcgagga ccaagtgctc
    10081 gagatcttca aagcagaccc cgacaccaaa ggttcgcgcg ggcgctggta cgagcgtgtg
    10141 aaggtgatcc gaccggggct ttcggaggag caagcggatg cccagggcat cacgacgggc
    10201 atggtcgccg acgccatccg aagcacgttc gaagggaaac aagaaggtgt ctaccgtgag
    10261 ggcgacgagg tcatccccgt cgtcttccgg caacctgctc cgactcgact cgacgtcgga
    10321 aatctgaggc agattcccat ctggagcccg atggcccagc gatacgttcc gctggcgaac
    10381 gtggtgaccg agctcgaaac ggagtggacc gaccaggtgg tcgagcgccg caaccgaaag
    10441 cgaaccttga ccgtggttac ggaccccacc gaggactccg cgcctacgct ctggaatcga
    10501 ctcgcgcccc aggtcgaggc gctttcgttc ccgcccggct actacgtcga atggggtggc
    10561 gagtacgaat cccaacagga cgcgcaagcg gcgctctttg cacc
//
LOCUS       DASRZF010000409        11728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2038792, whole genome shotgun sequence.
ACCESSION   DASRZF010000409 DASRZF010000000
VERSION     DASRZF010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 11728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11728
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2038792"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1345)
                     /locus_tag="VFG22_18090"
     CDS             complement(<1..1345)
                     /locus_tag="VFG22_18090"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HET6418212.1"
                     /translation="MRSQGAAFAFEGAPARETSREFLERALGRRTSEAENLIVTVPAP
                     LAPPDALLRAFPHEASILWDPPNSDACSGLGVAATLAPKASTMAEFFDRTNHVADAAS
                     PLPRVFGGSAFAPGAARHAPWDSFGDGFLLLPTWTYHAGERAWLSLAVCGTNGPDTQE
                     VLDVFDTIWDSLERPIGVIPSPVSVRRVDEADRQTWSRQVEDIRQRIQNGEFEKVVAA
                     RHCVVELETGAGSLDVLKRLEERFPGCTRFALWRGDSAFLGATPELLISRRGPSVLSE
                     ALAGSTAHGDAARMMASSKEREEHQLVVRAILRALEPYCESLRSAPEPSVRELPNLLH
                     MQTPIEGRLREPTHILSLVRALHPTPAVGGVPTQEAIRWIVEHEALARGWYSAPVGWA
                     NAAGDGEFVVALRSGLLHDGKAWVYAGAGIMADSDPEAEYTETELKMQALLGALHG"
     gene            complement(1342..2073)
                     /gene="ubiE"
                     /locus_tag="VFG22_18095"
     CDS             complement(1342..2073)
                     /gene="ubiE"
                     /locus_tag="VFG22_18095"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HET6418213.1"
                     /translation="MSEALRQTSSPNPGSGEMFDQIAGRYDLLNRVMSMGIDQSWRRK
                     TVAAMELPAGAQVLDLATGTGDLALMIARLHPDATVIGSDPSSRMLEVGVQKVARVGL
                     ADRVSLELGDAQALPYEDDAFDGCCIAFGIRNVPDRSAALAEMARVTRPGGRVAVLEL
                     GEPSVGWLSPIARFHVRKVIPKIGGMLSGSEEYKYLQQSIAAFPAADRFAEQMQASGL
                     NVLEVKPLTFGACTLFVAEPQESLS"
     gene            complement(2070..3284)
                     /locus_tag="VFG22_18100"
     CDS             complement(2070..3284)
                     /locus_tag="VFG22_18100"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0016054 - organic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HET6418214.1"
                     /translation="MSKQPPAVTSRIPGFYREHVEQRLRTMIETGLLSEDSVRHLQDG
                     GRLTLEVADHMSENVIGVHGLPLSVALNFRVNNNDVLVPMAVEEPSVVAAASNAARQV
                     RMTGGFFGEATAPIMTTQVQFDDVPNPEKAKARVEAERELIFQIADEAIPGMVSRGGG
                     CRDLDVRVLDEDDGVLVVHLYVDVGDAMGANVVDAVAEAVAPRIQELTGGTVGLRILS
                     NLPMRRRVKVRCDVSVDVLGGPELADGIAKASRFAELDPFRAVTHNKGVMNGVDAVAL
                     AMGQDWRSIEAGAHAFASVTGTYKPLVVWTRTETGVHGELDMPMAVGTVGGSTRVHRG
                     VRAGFELMGVKNAGELAIVMGAAGMASNLAALRALAGEGIQRGHMRLHSRKQEVHQNA
                     RVQAKKVERGAQ"
     gene            complement(3309..4379)
                     /locus_tag="VFG22_18105"
     CDS             complement(3309..4379)
                     /locus_tag="VFG22_18105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET6418215.1"
                     /translation="MSLIAAVRSDSSSDVLSSLQAVCESQGLGDLSAHLADLAELVSW
                     DMTALEQGIQSLPVGRSVVHKSAHHLLEVAGKRLRPMCVVLASRLGRGLDEKTREFGV
                     AVELVHCATLLHDDVVDAGDQRRGVPSARTLYGNAASIFAGDWLLVDALRRVRGAEMP
                     DVLVRLLDIIDEMIFAEAIQLENRGRIDARMRTYMQVVEGKTAALFRWAMFAGARAGG
                     LDPEACKALEEYGLHLGVAFQLIDDHLDYAGDGSTIGKSPFTDLREGKMTHPVIVALE
                     RDSSLKRVLEELIASPVDEVPEEAKARLLTALNDTGALESTRELAIRKADEACAALGA
                     LPDSRARNALFTVARATVHRES"
     gene            complement(4411..4902)
                     /locus_tag="VFG22_18110"
     CDS             complement(4411..4902)
                     /locus_tag="VFG22_18110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418216.1"
                     /translation="MGRLMEFWGFRRHMGRLWTVLYLSPDPMTTAELSDTLKLSSSAV
                     SLSLGELVRWGAVRKTWLPGERKDFYQAESSVWKLLRRVYERRELNLIREAIDAFGDA
                     QRHLDETRAQLSVRDRRRVDFMRKRLSRLSALSKAGERLVRLLVAGRMLNPADLQSVE
                     EED"
     gene            5107..6789
                     /gene="ilvD"
                     /locus_tag="VFG22_18115"
     CDS             5107..6789
                     /gene="ilvD"
                     /locus_tag="VFG22_18115"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF002068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET6418217.1"
                     /translation="MARKLNKYSSRITEPPSQGASQAMLHATGLTRADLKKPEVGISA
                     VWYEGNSCNMHLNDLAAFVKRGVVDAGLVGMRFNTIGVSDGISMGTDGMSYSLQSRDL
                     IADSIETVMSAQWYDANISIPGCDKNMPGCMIAIGRLNRPALMIYGGTIRAGRGSEGE
                     KLDIVSAFQSYGELLAKSIDEPKRLDIIEHACPGAGACGGMYTANTMASAIEAMGMSL
                     PYSSSTPAEDPAKQEECLRAGEAIKLLLEKDIKPRDIMTREAFENAMVTIMALGGSTN
                     AVLHLIAMARSVDVPLTIDDFQTVSDRVPFLADLKPSGQYVMEDLHAVGGTPAVLRYL
                     LAHGALDGSCLTVTGKTLAENLAELPDLSEGQDVIHPWDAPIKATGHLTILRGSLAPD
                     GSVAKITGKEGYAFRGPAKVFDCEEEMLAAMERGEIGKGNVVVIRYEGPKGGPGMPEM
                     LSPTSVIMGAGLGQDVALITDGRFSGGSHGFIIGHITPEAQDGGPIALVQDGDIIAID
                     AKKRCIDLELDDAELARRRAAWKAPALKATRGTLYKYIKNVKSASEGCVTDE"
     gene            6923..8080
                     /locus_tag="VFG22_18120"
     CDS             6923..8080
                     /locus_tag="VFG22_18120"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET6418218.1"
                     /translation="MYINKRGLREIGNVLFKRPTPGRLVTSAWIAPIYLGLRQTAAAF
                     RALDEVLYPEYRDQKVERPVFVFANPRSGTTLAHRLISMDDEVFTTVKLYQTIFPAIT
                     ATKTFKRLVGFSNTRLGSVLQRAYDFFNAPLESRWQDVHQISLDQAEEDVCTLLWSLE
                     SPATGLLFPFMDELPSQTWIDRQSDEERRAFMDCYENTIKRHVYEAGGKRFLNKNVFF
                     STRVRSVYEKFPDAVFVYLVRDPQDCLPSFLNMFYRAWKAHSPSIRPDGEEIQALKRL
                     GYDYYRYALECRKGIPDDQFIVIRYEDLVRDPKKTVLDLYERLGMPVSQAFKAKLDDA
                     MIAHSEYESSRSVELDFFGITREEVSEELSEVLEELGYDAPGSAQYLEAAE"
     gene            8171..9391
                     /locus_tag="VFG22_18125"
     CDS             8171..9391
                     /locus_tag="VFG22_18125"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/OsmC family
                     protein"
                     /protein_id="HET6418219.1"
                     /translation="MTTDLEQFEFTGGDGQVLAARLHLPAVEPVACAVFAHCFTCSKD
                     SRAARYISSALAEKGIATLRFDFTGLGDSEGEFESTTFSHNVTDIVAAARALGEMYEA
                     PILLVGHSLGGTAVLAAASRIPNAVGVATVAAPFRPEHIARLFEGSLDQIQSHGEADV
                     QIAGRNFTIRQAFIDDIREHCSAERIAALGRPLLLFHSPQDNVVGIDNAREIYQAARH
                     PKSFVSLDGADHLLGRPSDARYVAEVLAAWASRYLPGRAAEEIPENTPEGEVVVEGKT
                     SGFLQRVRARHLVFAADEPLEKGGTNLGPNPYELLLAGLGACTSMTLRMYAGRKEWPL
                     DSVRVTLRHDRVHAKDCEDCEQDTGMIDIIDKKLELEGELSEEQRQRLLEISARCPVH
                     RTLLNEIKIRSELV"
     gene            9388..11376
                     /locus_tag="VFG22_18130"
     CDS             9388..11376
                     /locus_tag="VFG22_18130"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET6418220.1"
                     /translation="MTTLASVHDVSLGLGGRTLFDGLSFQVHQGDHIGLVGPNGSGKS
                     SLFRLLAGSIEPDGGSVKTAPGTRIGFLPQEIASFPDEKVLTFVYDSVPGRPELRADL
                     QRHEARLAELEHAREEHREELVAVASEIAELHERIAALDARFSEHEAKRILHGLGFTQ
                     EDHDRSLSVLSGGWKMRAVLASLLFQKPGLLLLDEPTNHLDMPSVAWFSKFLSSYDQA
                     LLLVSHDREFLNEQVNRVISFEPEGVRSFRGNYESYLAQREEERELLENRAKNLEREK
                     AHLEQFVRRFRAKASKAAQVQSRVRKLEKMDTVELHRNAREVRFSFPPTERGARVPMR
                     VDELSKSFGEHVVLDQVSLAVERGDRIAIVGPNGAGKTTLLKILAGEIDASGGRVHLP
                     GQSNVRYFAQHHAEALNLERTVLQEVSYASDGTSVERVRKVLGALLFDELAIDKPVSV
                     LSGGERARVALAKILVRPSNVLLMDEPTNHLDLQTTEALTDALATYDGTLLFVSHNRA
                     FIRRLATKLWVVHDGGVESYPGTLDDYLWSCQERDVAPATDAAIKPVAQKRSRQARKE
                     QRRKEAEIRAKQNQGVKPLEKRVTKLEQTIAKLEQTIAAHNEELSKPEVYDDPERRDT
                     LLREVREAQSALDRSFADWTESHEKLEELRAELEVAIG"
     gene            complement(11373..>11728)
                     /locus_tag="VFG22_18135"
     CDS             complement(11373..>11728)
                     /locus_tag="VFG22_18135"
                     /inference="COORDINATES: protein motif:HMM:NF021355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HET6418221.1"
                     /translation="NYNHKAFNFYADRGLLAFPFVSYEGDFSSTLELWDVSAADGFTR
                     RGAVDHSDLVLDDCGGVPYPVEDFYYNCGYQPQITRGVFISDDTTDYIYSISYGGIRV
                     HDMSDLATPLATATY"
BASE COUNT         2349 a         3676 c         3643 g         2060 t
ORIGIN      
        1 cgccatggag cgctccaaga agcgcctgca tcttgagctc ggtctcggtg tattcggcct
       61 cgggatcgga gtcggccatg attccggcgc cagcgtacac ccaggccttc ccgtcgtgaa
      121 gcaatccgga gcgaagggct accacgaact cgccgtcccc tgcagcgttt gcccatccaa
      181 ctggggcact ataccagcca cgtgcgaggg cttcatgctc gacgatccac cggatcgcct
      241 cctgcgtcgg aacgccgccg accgccggcg tcgggtgcaa agcccggacg agggaaagga
      301 tgtgcgtggg ctcgcgaagg cggccttcga ttggggtctg catgtgcagc agatttggaa
      361 gctcccgaac gctcggttcg ggtgcagatc gaagtgactc acaatacggc tcgagtgcgc
      421 gcaagatggc tcgaaccacc agttgatgct cctcgcgctc cttcgagctc gccatcatcc
      481 gtgccgcgtc cccgtgggca gtcgagccag cgagagcttc gctcagaacg gatggcccac
      541 gccgcgaaat caagagctcc ggggtggcgc ccaagaaggc ggagtcgcct cgccaaagcg
      601 cgaagcgcgt gcatccggga aaccgctcct ctagccgctt gagaacgtcg agtgaaccgg
      661 ccccagtctc gagctcgacg acgcagtgcc gtgccgcgac gaccttttcg aactctccgt
      721 tctgaatccg ttggcgaatg tcttcgacct gacgcgacca ggtttgccga tcggcttcat
      781 cgacccggcg aaccgacacc ggtgagggaa tgacgccaat cggtcgttcg aggctgtccc
      841 agatcgtgtc gaacacatcg aggacctcct gggtgtccgg gccgttagtc ccgcaaaccg
      901 cgagcgacaa ccaggcgcgc tcacccgcat gatacgtcca ggtcggcaag agaaggaaac
      961 cgtcgccgaa gctgtcccag ggcgcatgtc gcgccgctcc tggcgcaaac gcggagcctc
     1021 caaacactcg aggcaacggg ctcgccgcgt cggccacgtg gtttgtgcga tcgaagaact
     1081 cagccatggt cgacgctttg ggcgccaagg tagctgcaac gccaagaccc gagcaggcgt
     1141 cgctgttagg cggatcccag aggatgctcg cctcgtgagg aaacgcccgc aggagggcgt
     1201 ccggtggagc aaggggggcc ggaacagtga cgatcagatt ctcagcctcc gacgttcgcc
     1261 gaccgagcgc tcgttccaag aattcgcgag aggtctcccg agccggagcg ccctcgaaag
     1321 cgaaagctgc gccctgggac ctcatgacag ggactcctga ggctcggcaa cgaacagagt
     1381 gcaggcgccg aatgtcagcg gcttcacctc gagcacattc aggccggaag cctgcatctg
     1441 ctccgcgaac cgatccgccg cgggaaatgc agcaatcgac tgctgaaggt acttgtactc
     1501 ctcggagccg gatagcattc cgccgatctt cggaatcact ttccgcacgt ggaaacgcgc
     1561 gattgggctc agccaaccga cgctcggctc accgagctcc aggaccgcaa ctcgaccacc
     1621 gggcctggtc acgcgtgcca tctccgcgag cgcggcgctt cggtcgggga cattgcgaat
     1681 gccgaaagcg atacagcatc catcaaacgc atcgtcttcg tacggcaggg cctgcgcgtc
     1741 accaagctcg agcgaaactc ggtcggcaag gccgacgcgc gcgactttct ggacgccgac
     1801 ttcgagcatg cggctcgacg ggtcactccc gatcaccgtc gcatcgggat gcagcctcgc
     1861 gatcatcagg gcgaggtccc cggtgcctgt ggccaaatcg aggacctgtg ctccggcggg
     1921 aagctccatc gcggctaccg tcttgcggcg ccaagactga tcgatgccca tcgacatcac
     1981 tcggttcaag agatcgtacc ggccagcgat ctggtcgaac atctcgccag aaccggggtt
     2041 aggcgacgag gtctgccgaa gcgcttcgct cactgtgcac ctcgttcgac cttcttggcc
     2101 tgaacccttg cgttctgatg cacctcctgc tttcgggagt gaagacgcat gtggccacgt
     2161 tggatgccct cccccgctag cgcgcggagc gcggcgaggt tcgacgccat gccagccgcg
     2221 cccatcacga tggccagctc gccggcgttt ttgacgccca tgagctcgaa accagcgcgg
     2281 acaccgcgat gcacccgcgt cgagccgccc acggtaccga cggccatcgg catgtcgagc
     2341 tcaccgtgaa cgccagtctc cgtgcgggtc cagacgacca gtggcttgta cgtgccggtc
     2401 accgaagcaa aggcatgcgc gccggcttcg atggagcgcc aatcctgtcc catggcaaga
     2461 gcgacggcat cgacgccgtt catgacgccc ttgttgtgcg tgaccgctcg aaacgggtcg
     2521 agctctgcga agcggctggc cttcgcgatg ccgtcagcca gctcgggtcc accgagcacg
     2581 tcgacgctca cgtcgcaccg gactttgacc cggcgcctca tgggaagatt gctgagaatc
     2641 cgaagcccga cggtgccgcc ggtgagctct tgaatgcgcg gtgcgactgc ctcggcaacc
     2701 gcatcgacga cgttcgctcc catcgcatcc ccgacgtcga cgtagaggtg caccacgagc
     2761 accccatcgt cctcgtcgag aacgcgcacg tccaggtcac ggcagccacc gccgcgcgac
     2821 accattcccg gaatggcctc atcggcgatc tgaaagatca gctctcgctc ggcctcgact
     2881 cgcgccttcg ccttctcggg gttcggcacg tcatcgaact gaacctgggt ggtcatgatg
     2941 ggcgcggtgg cttcgccgaa gaatccgccg gtcatccgaa cctggcgcgc agcgttcgaa
     3001 gccgccgcaa cgacagatgg ctcttccacg gccatgggca ccagaacgtc gttgttgttg
     3061 actcgaaaat tgagtgcgac cgaaagagga agaccgtgca cgccgatgac gttctcgctc
     3121 atgtggtcgg caacctcgag tgtgagtcgc ccgccatcct gcaaatgccg aaccgagtcc
     3181 tctgacaaga gccccgtttc gatcatcgtt cgaaggcgtt gttcgacatg ctcgcggtag
     3241 aaccccggga ttctgctggt cacggctggt ggttgcttag acatcaatga ctctttcttc
     3301 gttgctcgct aggactcccg gtgtacggtc gctcgggcaa ccgtgaacaa tgcgtttcgc
     3361 gcgcggctgt cgggcaacgc acccaacgcc gcacaggctt cgtcggcctt ccgaattgcg
     3421 agctcgcgcg tcgattccag cgcgccggtg tcgttgaggg ccgtaagcaa gcgggccttg
     3481 gcctcttcgg ggacttcgtc gaccgggctt gcaatcagct cttcgagaac gcgtttcagt
     3541 gaggaatcgc gctcgagggc gacgatcact gggtgcgtca tcttcccttc gcgcaggtcg
     3601 gtgaagggtg acttcccgat cgtcgacccg tcacccgcgt agtccaggtg gtcgtcgatc
     3661 agttgaaacg cgacgccgag atggagtccg tactcctcga gcgccttgca ggcctcggga
     3721 tcgagcccac ctgcgcgggc cccggcgaac atcgcccagc ggaatagcgc ggcggtcttg
     3781 ccctctacga cctgcatgta cgtgcgcatc cgagcatcga tgcgccctcg attctcgagc
     3841 tgaatggctt cagcgaaaat catctcgtcg atgatgtcga gcaggcgaac caagacgtct
     3901 ggcatttcag cgccgcgcac gcggcgaagc gcgtccacga ggagccaatc accagcgaaa
     3961 atagaagccg cattgccgta gagtgttcgc gccgagggca ctcctcgacg ttgatcgcca
     4021 gcgtcaacga cgtcatcgtg aagaagcgtc gcgcaatgca ccaactcgac ggcaacgccg
     4081 aactcccgtg tcttttcgtc gagcccccgg ccgagccgcg aagcgagaac gacacacatc
     4141 ggacggagtc ttttccccgc gacctcgagg agatggtgcg cgctcttgtg gacgacgctc
     4201 cgcccaacgg gaaggctttg aataccctgt tcgagagcgg tcatgtccca actcacgagc
     4261 tccgccaggt cggccagatg cgcagagaga tcaccgagcc cctgcgactc gcacacggcc
     4321 tgaaggctcg agagcacatc ggagctcgaa tcggatcgga ctgccgctat caatgacatc
     4381 gcagggctcc gctgagtctc gcctggtcca tcagtcctct tcttccacgg attggagatc
     4441 ggcagggttg agcatgcgac cggcgacgag cagtcggacc agccgttcgc cagcctttga
     4501 tagcgcactg agccgagaaa gtcgcttccg catgaagtcg actcgtcttc gatcccggac
     4561 gctcagctga gccctcgtct cgtcgagatg ccgctgtgcg tcaccgaagg cgtcgatcgc
     4621 ctcgcggatc aggttcagct cccggcgctc gtagactctt cggagcagct tccagacgct
     4681 actttcggcc tggtagaagt ccttgcgctc tccggggagc caggtcttgc gaacggcgcc
     4741 ccagcgcacc agctctccga gcgaaaggct caccgcgctg ctgctgagct tgagggtgtc
     4801 cgagagttcg gccgtggtca tggggtcggg cgaaaggtaa agaacggtcc agagccggcc
     4861 catgtgacgg cgaaaccccc agaactccat gagacggccc atggcctcgg cagcctgcag
     4921 ctcggcggct cgccataaag gctcggaatc atttgattct tgttgtttta cggcggcgct
     4981 ggacactcgg gcaactcagt tccttcaaat tttcttgaac agacgtagga ccggggctgg
     5041 agcgcgtcaa gccaacggct gggctggcgg agtgagcggt ttggggtgct agactccgca
     5101 gccccaatgg ccagaaaact caacaagtac agcagtcgaa tcaccgagcc gccgtcgcaa
     5161 ggcgcatcgc aagcgatgct ccacgcgacc ggtctcacac gggccgatct gaaaaagccg
     5221 gaagttggca tttcagccgt ctggtacgag ggcaacagct gcaacatgca tctcaacgac
     5281 cttgccgcct tcgtgaagcg cggcgtggtg gatgcaggcc tggtcggcat gcgattcaac
     5341 acgattgggg tgtcagacgg aatctcgatg ggcaccgacg gaatgagcta ttcgctccaa
     5401 tcgcgcgacc tcattgccga ctcgatcgag acggtgatgt cagcgcagtg gtacgatgcc
     5461 aatatctcca tcccgggttg tgacaagaac atgccgggtt gcatgatcgc gatcgggcgg
     5521 ctcaatcgcc cagcgctgat gatctacggc ggcacgattc gcgctggacg cggaagcgag
     5581 ggcgagaaac tcgacatcgt gtccgctttt cagtcctacg gagagctgct ggctaaatcg
     5641 atcgacgagc ccaagcgtct cgacatcatc gagcacgcct gccctggcgc gggcgcctgc
     5701 ggcggaatgt acacggcgaa caccatggct tcggcaatcg aggcgatggg catgtccctg
     5761 ccctacagct cctcgactcc cgcagaggat ccggcaaaac aggaggaatg cctgcgcgcc
     5821 ggggaagcga tcaagctgct cctcgaaaaa gacatcaagc cgcgagacat catgacgcgc
     5881 gaagcgttcg agaacgcgat ggtgacgatc atggcgctcg gcggttcgac gaatgccgtc
     5941 ttgcatctga tagccatggc gcgctccgtc gacgtgccgc tcacgatcga cgactttcag
     6001 acggtgagcg accgtgtgcc ttttctcgcc gatctcaagc ccagcgggca gtacgtgatg
     6061 gaggacttgc acgcggtggg cggcacacct gcagtgctgc gatatctgct tgcgcacgga
     6121 gcgctcgacg ggagctgcct gaccgtcacc gggaagacgc ttgctgaaaa cctcgcggag
     6181 ctacccgacc tcagcgaggg acaggacgtg atccaccctt gggacgcgcc aatcaaggcg
     6241 acgggtcacc tcacgatact tcggggtagc ctcgctcccg acggatcggt cgccaagatc
     6301 accggcaagg aaggctacgc gttcagaggc cctgccaaag tgttcgactg tgaagaagag
     6361 atgctcgccg cgatggaacg cggcgaaatc ggcaaaggca acgtggtggt cattcgctac
     6421 gagggaccga agggcggacc ggggatgccc gagatgctct cgccaacgtc ggtgatcatg
     6481 ggcgccgggc tcggtcagga cgttgcgttg atcaccgatg gacgcttctc cggcggctca
     6541 cacggtttca tcatcgggca catcacgcca gaggcgcagg acggcggtcc gatcgctctc
     6601 gtccaagacg gcgacatcat cgcgatcgat gccaagaaaa gatgcatcga tctcgagctc
     6661 gacgacgccg aattggcaag gcggcgcgcc gcatggaaag cgccagcact gaaagcgacc
     6721 cgtggaacgc tttacaagta catcaaaaat gtaaaatcgg cttccgaggg ctgcgtcacc
     6781 gacgaatgag ggagccgctc aatcgcgagc cctgaccgct cgcgcgccct gcggcaccgc
     6841 tcgcccggtt ttccccgcaa tttcgagccc cgaccctaga gtgaaggagg gcaagcgggg
     6901 atcgagccgc aggatagcga cgatgtacat caacaaacgg ggcctccgag aaattggaaa
     6961 cgtgctcttc aaacgaccga ctccgggccg cttggtcacg agcgcatgga tcgcgcccat
     7021 ctatctcggc ctccgacaga cggctgccgc ttttcgcgcg ctggacgagg tcctgtaccc
     7081 cgagtaccgc gatcagaagg ttgagcgccc ggtcttcgtc ttcgccaatc ctcgaagcgg
     7141 caccacgctc gcccaccggc tgatcagcat ggacgacgag gtcttcacca ccgtgaagct
     7201 gtaccagacg atattcccgg cgatcacggc aacgaagact ttcaaacgcc tggtcggatt
     7261 ttcgaacaca cggttgggct cggtgcttca gcgcgcgtat gacttcttca acgctccgct
     7321 cgaaagccgg tggcaggacg tccaccaaat cagtctggat caggccgagg aagacgtctg
     7381 cacgcttctc tggagcctcg aatcgccggc gaccggcttg ctcttcccgt tcatggacga
     7441 gctgccgagc cagacttgga tcgaccgcca gtcggacgag gaacggcgcg cgttcatgga
     7501 ctgctacgag aacaccatca aacgacacgt gtacgaagcc gggggaaagc gcttcttgaa
     7561 caagaacgtt ttcttttcga ctcgcgttcg ctcggtctac gagaagttcc ccgatgcggt
     7621 gttcgtgtac ctcgtccgcg acccgcaaga ctgcctccca tcgtttttga acatgttcta
     7681 cagggcctgg aaggcgcact caccgagcat ccgccccgac ggtgaagaga tccaagccct
     7741 caagcgcctc ggctacgact actatcgata tgcgctcgag tgccggaaag ggatccccga
     7801 cgatcagttc atcgtgattc gctacgagga cctcgtccgc gacccgaaga agacggttct
     7861 agacctctat gagcggctcg gcatgccggt cagccaggcg ttcaaagcca agctcgacga
     7921 tgcaatgatc gcgcacagcg agtatgaaag ctcacgcagt gtggagctcg acttcttcgg
     7981 gatcacccgg gaagaagtct ccgaggagct cagcgaggtt ctggaagagc ttggctacga
     8041 tgccccggga tccgcgcagt atctggaagc cgctgaatag caaccctttg gcgttttctt
     8101 tcgggcgagc gtggtgctgg gtaacgcccg ggcgcaagcg cggtacattt ggggactctt
     8161 gggaggctcc atgacgacgg atcttgaaca gtttgaattt acgggtggcg acggccaggt
     8221 gcttgcagcc cggcttcact tgcccgcggt cgaacccgtt gcctgcgcgg tcttcgccca
     8281 ctgcttcacg tgctcgaagg acagccgggc cgcgaggtac atcagctcag ctctcgcaga
     8341 aaaggggatc gctacgttgc gcttcgactt cactgggctc ggcgacagcg agggggagtt
     8401 cgagagcacg acgttttcac acaacgtcac cgacatcgtc gcagcggcgc gagcgcttgg
     8461 cgaaatgtac gaggcaccga tcttgctcgt cggacacagc cttgggggca cggcagtcct
     8521 cgcggcggcc agccggatcc cgaatgcggt cggagttgcg accgtcgccg cgccgtttcg
     8581 gccagaacat atcgcacgct tgttcgaggg gtcgctcgac caaattcaat cgcatggcga
     8641 ggcggacgtg cagatcgcgg ggcgaaattt cacaatccgc caggccttca tcgatgacat
     8701 tcgggagcac tgcagcgccg aacggatcgc agcgctcgga aggccattgc tgctgttcca
     8761 ttcgccgcag gataacgtcg tcggaatcga caatgcccgg gagatctatc aagcagcgcg
     8821 gcacccgaag agcttcgtct cgctcgatgg cgcagaccac ctgctcggcc ggcctagcga
     8881 cgcgcgatac gtcgccgagg ttctggcagc ctgggccagc cgctatctgc ccggaagagc
     8941 tgccgaagag atccccgaga acactcctga gggagaagtc gtcgtcgaag ggaaaacgag
     9001 cgggttcctg cagcgcgttc gggcccgcca cctcgttttt gccgctgacg agccgctcga
     9061 gaagggggga accaacctcg gaccgaaccc ctacgagttg ctcctcgcgg ggctcggcgc
     9121 ctgcacctcg atgactctca gaatgtatgc cggccggaag gagtggcctc tcgactcggt
     9181 ccgagtcacc ctgcgccacg atcgcgtcca cgcgaaggac tgcgaggact gtgaacagga
     9241 cacggggatg atcgacatca tcgacaagaa gctcgagctc gaaggagagc tcagcgaaga
     9301 acaacgccag cggctgctgg agatttccgc gcgttgcccc gtgcaccgta ccctgctgaa
     9361 cgagatcaag atccgctcgg agttggtttg acgacactcg cttcggtcca cgacgtttct
     9421 ctgggacttg gagggcgaac gctcttcgac gggctctcgt tccaggtgca ccagggtgat
     9481 cacatcggct tggtgggacc caatggctct ggcaagtcga gcttgttccg attgctggcg
     9541 ggaagcatcg agccagacgg tggttccgtc aaaacagcgc ccgggactcg cattggattt
     9601 ctgcctcaag agatcgcctc ttttccggac gaaaaagtgc ttactttcgt ttacgacagc
     9661 gtgccagggc gaccagagct cagagcagac ctccaacgcc acgaggcgcg cttggcggag
     9721 ctcgagcacg cgcgcgaaga gcaccgggaa gagctggtcg cggtcgcaag cgaaattgcc
     9781 gagctccacg agcgaatcgc cgcactcgat gcgcggttct cggagcacga ggcaaagaga
     9841 atcctacatg gactcggctt cactcaggag gaccatgatc gatccctctc ggtactgagc
     9901 ggaggctgga agatgcgcgc ggtcttggcc tcgcttctgt ttcagaagcc tggcctgctg
     9961 cttctcgatg agcccaccaa ccatctcgac atgccatccg tggcttggtt ctcgaagttc
    10021 ctgagcagct acgatcaggc ccttctgctg gtctcccacg atagggagtt tctgaacgaa
    10081 caggtcaacc gcgtgatcag cttcgaaccg gagggcgtgc gatcgttccg tgggaactac
    10141 gaatcgtatc tggcgcagcg agaagaagag cgcgagcttc tcgagaaccg ggccaaaaac
    10201 ctggaacgtg aaaaagccca cctggagcag ttcgtgcgcc gtttcagggc gaaggcgagc
    10261 aaggctgcgc aagttcagag ccgggtgcgc aagctcgaga agatggacac ggtggagctt
    10321 catcgcaacg cgcgtgaagt gcgcttctcg tttccgccaa ccgagcgcgg agcgcgcgtg
    10381 ccgatgcgcg tggacgagct cagcaaatcc tttggcgagc acgtagttct cgaccaggtc
    10441 tcactcgcgg tggagcgcgg agatcggatc gcgatcgtgg ggccgaacgg cgccgggaag
    10501 acgacgctcc tcaagattct cgctggcgag atcgacgcca gcgggggacg cgttcacctg
    10561 ccgggtcaat cgaatgtacg gtacttcgcg cagcatcacg ccgaggctct caacctcgag
    10621 agaacggtcc tgcaggaagt ctcatatgca agcgatggga cgagcgtgga acgggtgcgg
    10681 aaggtcctcg gagctctcct cttcgacgag ctggcgatcg acaagcccgt ttcggtgctg
    10741 tctggaggcg agcgcgcgcg agtcgcgctg gcaaagatcc tcgtgcgccc aagcaatgtg
    10801 ctgctcatgg acgagccgac caaccacctc gaccttcaaa cgaccgaggc cctcaccgat
    10861 gcgctcgcca cttacgacgg aacgctgctc ttcgtgagcc acaaccgggc cttcattcgg
    10921 aggctcgcga ccaagctatg ggtcgtgcac gacggtggag tcgaaagcta tccgggcacc
    10981 ctcgacgact acctctggtc ctgccaggag cgggacgtgg caccggcaac cgatgcggcc
    11041 atcaaaccgg tggcccagaa gcgaagtcgc caggcccgaa aggaacagcg ccgcaaagag
    11101 gcagagattc gcgccaaaca gaaccaggga gtgaagcctc tggagaagcg agtcaccaag
    11161 ctcgagcaga ccatcgccaa gctcgagcag acgatcgcgg cgcacaacga agagctcagc
    11221 aagcccgagg tctacgatga cccagagcgg cgagacacct tgctgcgcga ggtgcgagaa
    11281 gcccagtccg ccctcgatcg atcgtttgcg gactggaccg agtcccacga gaagctcgaa
    11341 gagcttcgcg cggagctcga ggttgcgatc ggctaatagg tcgcggtagc cagcggtgtc
    11401 gcgaggtcgc tcatgtcgtg cacccgaatg ccgccgtagc tgatcgaata gatgtagtcc
    11461 gtggtgtcgt cgctgatgaa gacgccccgc gtgatctgag gctggtagcc gcaattgtag
    11521 tagaagtcct cgacggggta cgggactcct ccgcagtcgt cgagaacaag atcggagtgg
    11581 tcgacggcgc cgcggcgggt gaaaccatcc gcagcactga cgtcccagag ctcgagcgtg
    11641 ctggagaagt cgccctcata ggacacgaac ggaaacgcta gcagtcctct gtcggcgtag
    11701 aagttgaacg ccttgtggtt gtagttcg
//
LOCUS       DASRZF010000410         2088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3748136, whole genome shotgun sequence.
ACCESSION   DASRZF010000410 DASRZF010000000
VERSION     DASRZF010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3748136"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..1534)
                     /locus_tag="VFG22_18140"
     CDS             complement(182..1534)
                     /locus_tag="VFG22_18140"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HET6418222.1"
                     /translation="MKPLGTVDTSFLLAEDRETPMHTGGLMLFSYPESAREDWLHQTL
                     SGNDDPKAVQYPFNQKLAWPLRRLGLPHWVTDEDLDTHYHVRHSALPKPGRYREMFSL
                     VSRLHSTRLHRDRPLWEAHIIEGVEDNRFALYMKTHHSMIDGVGAMRMLRACLSSDPN
                     KRDMPAPWNMPRPRRESIAAPNPRGAIAATAGAMGGVLKFAAAVRHRDKHGMIAPFQT
                     PLTILNGRVTGSRRLVAQSWSLLRIRTVAKALNATVNDIVLAMSGGALRRYLIDQGAL
                     PSEPLTAMTPVSFRPADAEVEGNSFSVVFASLATELGDPIDRLRAIQKSMGEAKRLLK
                     SMNTADIPIFTSIVALPVLVPHLLRISGRTRPTFNVTVSNVPGPQEQLYWNGAPLEGM
                     YPLSIVTHGQALNITVTSCGESLDFGIVACRRSVPSAQRLIDHMDAALVELEQRVERS
                     "
     gene            1610..1849
                     /locus_tag="VFG22_18145"
     CDS             1610..1849
                     /locus_tag="VFG22_18145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418223.1"
                     /translation="MSKILKYIVLGTAVLALSLSAACKEDASPTAKEISSGAETGAKV
                     GAEAGMDAASSGGAKEGVKTGGTEGGKAAASEIVN"
     gene            1868..>2088
                     /locus_tag="VFG22_18150"
     CDS             1868..>2088
                     /locus_tag="VFG22_18150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418224.1"
                     /translation="MEPKKSQGPGLAKIAGSALLVCLLLVGAAYLFKDRSQSASGEQG
                     SLATSRLQSILTRCEDTPVVDLDDGRERLR"
BASE COUNT          402 a          629 c          683 g          374 t
ORIGIN      
        1 cgaaaagcgt accaactcga cagtctcgtg gagcgctatg gcatcgatgt gccggagcga
       61 cacaccgccg caggtgacgc cctcgcaacc gcgctgctat ttcaacgctt gctgaagaag
      121 gccgggcgaa ggggaatccg cactcttggc gacctcttag cccactgaag gcgatacaca
      181 ctcacgatcg ctctactcgc tgctcgagct ctacgagcgc cgcgtccata tggtcgatca
      241 ggcgttgtgc gctcgggacc gaacggcggc aggccacgat tccgaagtcg aggctttcac
      301 cacagctcgt cacggtgatg ttcagcgcct ggccgtgcgt cacgatggag agggggtaca
      361 tgccctcgag cggcgcgccg ttccagtaga gctgctcctg cgggccgggg acgttggaga
      421 ccgtgacgtt gaacgtgggc cgcgtgcgcc ccgagattcg aagcagatgt ggaacgagca
      481 ccggaagagc cacgatgctc gtgaagatgg gtatgtcggc ggtgttcatg ctcttgagca
      541 ggcgcttggc ctcacccatc gacttttgaa tcgcgcgtag gcggtcgatc ggatcgccga
      601 gctccgttgc aaggcttgcg aagaccacgg aaaaggaatt gccttcgact tcggcgtcag
      661 ccggcctgaa cgaaaccggc gtcatcgccg tcagcggctc cgagggcaac gccccctggt
      721 cgatcaggta tcgccggagc gcgccgcccg acatcgcgag aacgatatcg ttgacagtgg
      781 cgttcagcgc cttggcgacc gtccgaatcc gcagcaacga ccaggactgc gcgacaagac
      841 gccgcgagcc cgtgacccgg ccgttcaata tcgtgagcgg cgtttggaaa ggagcgatca
      901 ttccgtgctt gtcacggtgg cgtaccgccg ctgcgaattt gaggaccccg cccatcgctc
      961 ccgccgtggc agcgatcgcc ccccgcggat tcggagcggc aatactttcg cgccgcgggc
     1021 gaggcatgtt ccagggggca ggcatgtctc gcttgttcgg gtcactgctg aggcatgcgc
     1081 gcagcattcg catcgcgccc acgccatcga tcatcgagtg gtgcgtcttc atgtagagcg
     1141 cgaagcggtt gtcttcgacc ccctcgatga tgtgcgcctc ccaaagtgga cggtctctat
     1201 gaaggcgcgt gctgtggagc cgagatacga gcgagaacat ctcgcggtag cggcctggtt
     1261 ttggcagcgc cgagtggcgc acatggtagt gcgtatcgag gtcctcatcc gtcacccagt
     1321 ggggcaggcc gagccgccgg agcggccagg ccagcttctg attgaacggg tactgcaccg
     1381 ccttcgggtc gtcgttccca gagagggtct gatgcaacca gtcttcgcgg gcgctttcgg
     1441 ggtagctgaa gagcatgaga ccccccgtgt gcatgggcgt ctcgcggtct tcggccagca
     1501 aaaagctggt atcgacggtt ccaaggggct tcatgggaca cagagaagta tcccacctgg
     1561 ccaagttttg gtaggctccg ccccactcaa ataggaggga aacagacaaa tgagcaaaat
     1621 tctcaagtac atcgtcctcg gaaccgcggt cttggcgctg agcctctcgg cggcctgcaa
     1681 ggaagacgcg agcccaacag caaaggaaat ttcgtccggc gcagagaccg gggccaaagt
     1741 gggcgccgag gcaggcatgg atgcggcatc gagcggcggc gccaaagaag gcgtgaaaac
     1801 gggcggaacg gaaggcggca aggccgcggc atccgagatc gtcaactaag caatcgatcg
     1861 tttcacgatg gagccgaaga agtcgcaggg gcccggtctc gccaagattg ccggtagcgc
     1921 cttgctcgtg tgcctcttgc tggttggcgc tgcgtacctt ttcaaggacc gcagtcagtc
     1981 agccagcggt gagcaagggt ctcttgcgac ttccaggctc cagtcgatcc tcacccgctg
     2041 cgaggatacg cccgtcgtgg atctcgacga tggtcgcgag cgcttgcg
//
LOCUS       DASRZF010000411        14015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2043168, whole genome shotgun sequence.
ACCESSION   DASRZF010000411 DASRZF010000000
VERSION     DASRZF010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 14015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14015
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2043168"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="VFG22_18155"
     CDS             <1..329
                     /locus_tag="VFG22_18155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HET6418225.1"
                     /translation="TTSLASLAAGNIAATGGIAQLLREQEFATQYHEGVRQNVHIQVV
                     SGRVILVVIFDNRTSLGLVRLRVKRATEHLTRIFEMVMAKAQDPFRNSPFTEITDDDI
                     DNLFND"
     gene            349..942
                     /locus_tag="VFG22_18160"
     CDS             349..942
                     /locus_tag="VFG22_18160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylation factor-like protein"
                     /protein_id="HET6418226.1"
                     /translation="MSFINYLSREINCKIVYYGPGLCGKTTNLQYIYNRTNPDAKGKM
                     ISLATETERTLFFDFLPLEIGEIRGFKTRFHLYTVPGQVFYDASRKLILKGVDGVVFV
                     ADSQMLRSEANTESMDNLRTNLAEQGYNLDTLPYVIQYNKRDMPNVSSVEELRYMLNT
                     GDVPDFGAVATTGEGVFETLKSVAKLVLTELKRGAAR"
     gene            complement(962..3409)
                     /gene="leuS"
                     /locus_tag="VFG22_18165"
     CDS             complement(962..3409)
                     /gene="leuS"
                     /locus_tag="VFG22_18165"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HET6418227.1"
                     /translation="MTRYDPATIEPKWQAFWDEDDTFRTTEGVDRPKAYILDMFPYPS
                     GSGLHVGHPEGYTATDIVARYHRAKGYDVLHPMGWDAFGLPAEQHAILTGTHPRDTTS
                     QNIATFRRQLKMLGFSYDWDREIDTTSPEYVKWTQWIFLQLFDRGLAYQSEIPVNWCP
                     ALGTVLANEEVIDGLSERGRHSVERVPLRQWVLKITAYADRLLEDLGEVDWPNSTRIM
                     QQEWIGRSEGAEVKFGVVGHDAAIEVFTTRPDTLFGATFMVLAPEHPLVDRVTTDAQR
                     SQVAAYVEQARNKSERDRQSSKEKTGVFTGGLAINPVNGAEIPIYIADYVLWGYGTGA
                     IMAVPAHDERDFEFATAHGIPIIEVVSKSGTASATPLNEAMVEQGIAVNSGKYDGLAT
                     AEFKKRVTGDLESNGQGRARVEYKLRDWNFSRQRYWGEPIPIYFPVETPGDPRAGADF
                     TIRYDEPIAVDESELPLRLPELDDYKPGEDPAGVLARATDWRFFQKDGQWFARETNTM
                     PQWAGSCWYYLRFIDPHNAEELCSPALAKEWLPVDLYVGGAEHAVLHLLYARFWHKVL
                     YDAGAVPTKEPFKKLVHQGMILGEGGQKMSKSRGNVVNPDDIVDEFGADSMRIYEMFM
                     GPLEATKPWSTSSIAGVRRFLDRVFNATLRADVKAKPDAALTRLLHQTIRKVTEDIEA
                     MRFNTAISAMMVLSNELTKLEQVPTEPLETLAKLLHPFAPHLGEELWEMLGQPASIQK
                     QPWPAYEQALCEEDEVEVPVQINGKVRGRITLPRDADQAQALAAAQGDPSLAAHLEGK
                     TLRKTIWVPAKILNLIV"
     gene            complement(3447..4532)
                     /locus_tag="VFG22_18170"
     CDS             complement(3447..4532)
                     /locus_tag="VFG22_18170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418228.1"
                     /translation="MRCLSFGVCLALLSLIACGGEEPSGSALEPRPEVQTCLSDLSAG
                     MPQRLSETGCFQDLASFEPGPDLIPYEVNSALWTDGAFKPRFMVVPSPKRIRIEDGGA
                     WTFPEGSILIKVFGFEFERGDPSSRRPVETRFMVLRDGVWEYTSYQWNEDGTDAERLD
                     EAKTVEYSIQDGNAVSVIEYQYPDYDACITCHGGAINEVLGPKTAQLNREHDYGGVVA
                     NQLRAMASIDLLELDAGEDFDPAMEPRMANPRLGEGSLEERARAYLDANCAHCHRPGG
                     WAPSDIGLDLRYEVTLEETGVCASMKYFGWAGRPRVLPGDPEGSGLVQRLTQEDALRM
                     PSLGTSIIDPFGVGLLSDWIARLQTCP"
     gene            complement(4582..5490)
                     /locus_tag="VFG22_18175"
     CDS             complement(4582..5490)
                     /locus_tag="VFG22_18175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HET6418229.1"
                     /translation="MDTPKPIAAALTYGTFPLIFGGVMGLGLYGVGQGWNPVATAMGL
                     TGAVVLLLLVLERVHPYQKDWLISHGDVRTDLIHNLVNFWIPELYTVVFVGGFTLAGA
                     GLSSALGMKLWPSGWPLLAQLLLALLVGELGTYWIHRWMHENPFLWRFHAAHHSAPRL
                     YWFNAARFHPMDLFAQQFLAVTPLVLLGADMRIIALHTLFTAIHGMFQHCNVDIRLGP
                     LNWFFSMAELHRWHHSKHLEEANTNYGANVILWDIVFQTRFLPQDRNPPTEIGIGDLP
                     RFPTGYWDQILSPFRWKEVEASARAK"
     gene            complement(5508..6257)
                     /locus_tag="VFG22_18180"
     CDS             complement(5508..6257)
                     /locus_tag="VFG22_18180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6418230.1"
                     /translation="MSNEATFDFSGAKVLVTGGSNGIGRAIAKAFAVAGAEVAITGTR
                     ASASEYDDDLSSYDYHPLQLTDKAGIESLAASLDRLDILVNNAGGNFPGGKHEAAPDV
                     FEESVAINLFGAYRLAVGCRDKLAASTLEGGASVINFASMAAFFAVPIVPGYGAGKAA
                     TVQMTKNLAASWAAEGIRVNAIAPGLVESNMTKIMKGVEPLEKPFIDRTPLRRWGAPE
                     DIAPVALFLASSSARFITGQTLLVDGGFSIT"
     gene            complement(6257..7261)
                     /locus_tag="VFG22_18185"
     CDS             complement(6257..7261)
                     /locus_tag="VFG22_18185"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET6418231.1"
                     /translation="MQQVKIHGPGKVELDEISPPEAGPRDAVVRVAACGVCGTDVRYV
                     RMGGLGRMMPLGHELAGVIETVGAEVTDLRPGARVVLNPTASGNMIGNGGPEGGFTHR
                     LLVRNAADGGCLFPIPDDLPMHLAALSEPLGVGMNAVNRSGVSAGDKVAIFGAGPIGL
                     SAIATLRDRGIDDVVAIDLSPRRLELARALGARATILASEQNPWVELKKLHGEAPLMG
                     APMAGTDAYIEASGAGSVITEVLQNAKAESSLTVVALHEQSVNVSFLWVMMKQMTIRG
                     AMEYPADFSDAVALLSRWDLSPMITHRFPLARIGEAIEVATDPGAGGKVMVDIDEGIA
                     "
     gene            complement(7261..8520)
                     /locus_tag="VFG22_18190"
     CDS             complement(7261..8520)
                     /locus_tag="VFG22_18190"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET6418232.1"
                     /translation="MTEQAADIRITDLRAPILTPVQKMVVDGFAGVEVELSVDAVLGE
                     AEKRTQLSDFGPADFQERLAVWCQAVDEDSELGPMGRARFFGDMVRYAANRLRFHDLI
                     GKHPEILEIEIREPIIIAGLPRSGTTHLLNLIAADSRLRSLPYWESLEPIPDPSVPFG
                     PKERDPRYLRCASEYQMLGQIAPLLKNMHDLAPDHIHEEIELAGLDFSSYQPEWLALV
                     PRWRDYYLAHDQRPHYQYMKNVLKALQWMHGPNRWVLKSPQHLEQLGPLVETFPDATV
                     ALTHRDPVSVVASAITMLTYGDRLRRTRVDPPAVAAYWIDRVEQLLRAAVRDRALLPE
                     SRSIDVLFHEFMSDDVAMVERIYQLANLPMNDEARAELDQYMTENPRGKHGRIVYDLR
                     GDFGVDPNALRERFEFYYERFPVQAEV"
     gene            complement(8517..9806)
                     /locus_tag="VFG22_18195"
     CDS             complement(8517..9806)
                     /locus_tag="VFG22_18195"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6418233.1"
                     /translation="MTSTPLHRSRPGGFEIRPASQPAAQRINEFIYVSQGLSNSYMIT
                     TQDGRVVINTGMGFEAPIHKRNFDAVDDSPVRYILLTQGHVDHVGGVDLIRDEGTEVV
                     AQAKNQAHQADDARIAAFRASRSAFAFADAIASAWKHIQENVGRGIPAQSKPTPEVTF
                     EERHSLELGGVRMELIATPGGETTDSMVVWLPDHKICFTGNLFSALFGHFPNLVTVRG
                     DRYREALGFIESLEKVLALEPELLLTGHHGPVVGRETIREELIRLRDAVQYVHDQTVR
                     GMNHGKDIHTLMREIRLPPELEVGEGYGKVSWSVRAIWENYAGWFHHRSTTELYAVPP
                     SSVHRDLVDLAGGANAVAARAQQRLIAGEPVEAIHLAEVALADSPSNVAALEVMIAAH
                     VQLEAESENFWLTQWLRKQLTKLRSTLHAAKSREEDS"
     regulatory      9865..9956
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            9977..11752
                     /gene="thiC"
                     /locus_tag="VFG22_18200"
     CDS             9977..11752
                     /gene="thiC"
                     /locus_tag="VFG22_18200"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HET6418234.1"
                     /translation="MTSFKIPLRGAHNPSSESAGTTPGSFANAPDIGGPRSFSSPDTP
                     GMPQPSDKTAWDFMPEDWRFENGHWTPPSGFEPVTQLEHARLGTVTAEMRRVAEREKH
                     LSPEQVRDEVAAGRMIIPANKVHLRHALDPMCIGRSGLTKINANMGASPVSSGTEEEV
                     EKLRWAERWGADTVMDLSTGGDLDATREAIIRNARVPIGTVPIYSMIIGRSIEELTLE
                     VILDTLRHQAEQGVDYFTIHAGVAREHLPFIRKRLIGVVSRGGSLLAKWMLHHGQENP
                     MFTHFEAICDVMREFDVSFSLGDGLRPGGLADATDDAQLGELDRLGELTERAWRKGCQ
                     VMVEGPGHVPFDQIEYNMKLQRQRCHGAPFYVLGPLVTDVFPGYDHITSAIGATAAGY
                     HGAAMLCYVTPKEHLGLPKREDVKQGCIAYKIAAHASDVALGIPGSRDWDDDLTRARA
                     ALNWEKHFDLAFDSDTARAFHDEDLDVDTDFCAMCGHDWCSVRISKEIVEFESGKASG
                     FVRERVMKSPALTPEQQEILRARGHLSPEEIHRLAGKTQHAVGAAEKAACHSDLVSDD
                     TQALRVQGEKLLQLRRKGTEGAPTA"
     gene            11778..12137
                     /locus_tag="VFG22_18205"
     CDS             11778..12137
                     /locus_tag="VFG22_18205"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET6418235.1"
                     /translation="MGKKLILGVMIGIVATAFGCSKESGGTTAYSLEDFDFPLVSTDV
                     AGGAETFAEFCEGCHPGGEKGDGPKISGEGISPSQMRWRVRSGGDDMPAFGPDKISNA
                     DLESLLAYAETLGAVSR"
     gene            complement(12540..12776)
                     /locus_tag="VFG22_18210"
     CDS             complement(12540..12776)
                     /locus_tag="VFG22_18210"
                     /inference="COORDINATES: protein motif:HMM:NF027672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4981 domain-containing protein"
                     /protein_id="HET6418236.1"
                     /translation="MQVDDFDFETGRMRITNRLDHIDLSERLVGHYVFTVDGEVLDEG
                     PFELPSLEPGSSAVVDLPSIEMAPPPGAEPRLRF"
     gene            complement(13138..13605)
                     /locus_tag="VFG22_18215"
     CDS             complement(13138..13605)
                     /locus_tag="VFG22_18215"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HET6418237.1"
                     /translation="MSYRDFINNFSVTPVGVWMAKTLASKVDPFLYKVSGGRFTSTGP
                     LTIPQLTLTTIGRKSGKARTVQLGYTEDGDDVLIVASNFGGTSHPAWSLNLDSNPAAS
                     ILLGAESKKVVATRLTDSEKAILWPKIADTIPQMKSYVKRTDRNIKVYRLSAK"
     gene            complement(13648..>14015)
                     /locus_tag="VFG22_18220"
     CDS             complement(13648..>14015)
                     /locus_tag="VFG22_18220"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HET6418238.1"
                     /translation="EMRGQLALPVQGSMRIGEASREGAPGLEFYGRPGGPVRAAADGR
                     VAFARNYGAYGLMVIIDHGNSFYTVYGGLGSTDVQTGDWVGMSTQIGTLDMGSPEAIL
                     FFEVRRGTRALDARSWLGL"
BASE COUNT         2661 a         4366 c         4334 g         2654 t
ORIGIN      
        1 acaccacgtc cctcgcctcg ctggcggccg gcaacatcgc agcgaccggg ggcatcgcgc
       61 agctgctccg ggagcaggag ttcgcaacgc aataccacga gggcgttcgg cagaacgtcc
      121 atattcaagt ggtgagcggc cgcgtgattc ttgtcgtcat cttcgataat cggacaagct
      181 tgggtctggt acgcttgagg gtgaagcggg ctacagaaca tctgacgcgg attttcgaaa
      241 tggtcatggc caaggcgcag gatccttttc ggaattcgcc gtttaccgaa ataaccgatg
      301 acgacatcga caacttgttc aatgattgac taggagaggc ttggggggat gtctttcatc
      361 aactacttat cgcgggagat caactgcaag atcgtgtact acggtccggg tctttgcggc
      421 aaaacgacca acctgcagta catctacaat cgcaccaacc cggatgcgaa aggcaagatg
      481 atttcgctcg cgaccgaaac cgagcgtacg ttgttcttcg acttccttcc gctcgagatc
      541 ggtgaaatcc gcggcttcaa gacgcgtttc catctgtaca ccgtgcccgg gcaagtgttc
      601 tacgacgcca gccgaaagct gatcttgaaa ggcgtcgacg gcgtcgtatt cgtcgccgac
      661 tcccagatgc tccgttccga ggccaacacc gagtcgatgg acaaccttcg caccaaccta
      721 gcggagcaag gctacaatct cgacaccctt ccctacgtca tccagtacaa caaacgagac
      781 atgcccaatg tctcttcggt ggaggagctt cggtacatgc tcaacaccgg cgacgtgccc
      841 gatttcggcg cggtggccac cacgggcgaa ggcgtcttcg aaaccctgaa atccgtcgcg
      901 aagctggttc tcaccgagct caagcgcggc gccgcgcgct gagccgcagc gcaggctgca
      961 tctagacgat caggttgagg atcttggcgg gcacccaaat ggtcttccgg agtgtcttgc
     1021 cctcgaggtg ggcggcgaga ctcggatccc cctgggccgc ggcaagagct tgggcttgat
     1081 cggcgtccct cggaagcgtg attcggccac ggactttgcc attgatctgg acgggcacct
     1141 cgacctcgtc ctcttcgcag agggcctgtt cgtaagccgg ccaaggctgc ttctggatgc
     1201 tggccggctg cccaagcatc tcccagagct cctcgcccag atgtggcgca aacggatgca
     1261 gaagcttcgc gagggtctcc aggggctccg tggggacctg ctctagcttg gtcagctcat
     1321 tgctgaggac catcatcgca ctgatggccg tgttgaaacg catggcctcg atgtcttcgg
     1381 tgaccttgcg aatcgtttgg tgcaacagtc gcgtcagtgc ggcgtcgggc ttggctttca
     1441 catcggcgcg aagcgtcgcg ttgaacacgc gatcgagaaa tcgtctcaca ccggcgatcg
     1501 agctcgtgct ccagggcttg gtagcctcga gcgggcccat gaacatctcg tagatccgca
     1561 tggagtcggc cccgaactca tccacgatgt cgtctggatt cacgacattg ccccgcgact
     1621 tgctcatctt ttggccgccc tcgccgagga tcatgccttg atgaacgagc ttcttgaagg
     1681 gctccttggt cgggaccgca ccggcgtcgt agagcacttt gtgccagaag cgggcgtaga
     1741 gaaggtgaag gacagcatgt tcggcgccgc ccacgtagag gtccacggga agccactctt
     1801 tggcgagcgc gggtgagcag agctcttcgg cattgtgcgg gtcgatgaag cgcaggtagt
     1861 accaacagga ccctgcccac tgcggcatcg tattggtttc gcgggcgaac cattggccgt
     1921 ctttctggaa gaagcgccag tcggtggcgc gggccaggac gcccgccgga tcctctccgg
     1981 gcttgtagtc gtctagctcg ggaagccgga ggggaagctc gctctcgtcg accgcgatcg
     2041 gttcgtcgta tcgaatcgtg aaatcagcac ctgcgcgagg gtctccgggc gtctccactg
     2101 ggaaatagat cggaatcggc tcgccccagt atcgctgacg agagaagttc cagtcgcgta
     2161 gcttgtactc gacgcgcgcc cggccctggc cgttcgactc gaggtccccg gtgacgcgct
     2221 ttttgaactc agccgtggcg agaccgtcat attttccgga gttcaccgcg atgccttgct
     2281 cgaccatggc ctcgttcaag ggcgtcgcgc tcgccgtgcc cgacttgcta accacttcga
     2341 tgatcggaat gccgtgagcc gtggcgaact cgaaatctcg ctcgtcgtgg gcgggcacag
     2401 ccatgattgc gccggtgccg tagccccaga ggacatagtc cgcgatgtag atcggaatct
     2461 cggcgccgtt gacgggattg atcgcgagcc cacccgtgaa gacgccggtc ttctccttgc
     2521 tcgactggcg gtcgcgctcg ctcttgttcc tcgcctgctc gacgtacgct gccacttggc
     2581 tccgctgcgc atcggtcgtg actcgatcga ccagagggtg ctccggcgcg agcaccatga
     2641 acgtagcgcc gaacaaggta tccgggcggg tcgtgaagac ctcgatcgca gcgtcatggc
     2701 cgactacgcc gaacttcact tcggcgcctt cagagcgacc gatccactcc tgttgcatga
     2761 tgcgcgtgct gttcggccag tccacttcac cgaggtcttc caggagacgg tcggcgtatg
     2821 cggtgatctt caagacccac tgccgaagcg ggacgcgctc gaccgaatgc cggccccgtt
     2881 cactcaatcc gtcgatcacc tcctcgttgg cgagcacagt accgagcgct ggacaccagt
     2941 tgacgggaat ctcgctctga taggccaggc cgcggtcgaa gagttggaga aagatccact
     3001 gcgtccattt cacgtattcg ggcgaggtcg tgtcgatctc gcgatcccag tcgtagctga
     3061 acccgagcat ttttagctgg cgacggaacg tcgcgatgtt ctgcgacgtg gtatcccgcg
     3121 gatgcgtccc ggttagaatc gcgtgttgct cagcgggcaa cccaaacgcg tcccagccca
     3181 ttggatgaag cacgtcatac cccttggcac ggtgatagcg cgcgacgatg tcggtcgcgg
     3241 tgtatccctc cggatgaccc acgtgcaggc ccgaccccga gggatacgga aacatgtcga
     3301 gaatgtacgc cttcggacga tccactccct cggtcgtgcg aaaggtgtcg tcctcgtccc
     3361 agaaggcctg ccacttgggc tcaatcgtcg ctggatcgta gcgcgtcatc gggcctgcca
     3421 tagcatgccc gcgcagagcc cgcgatctac ggacaagtct ggagacgagc gatccaatca
     3481 ctgagcaggc ccacaccgaa tggatcgatg atcgaagtcc cgagcgatgg cattcgtagc
     3541 gcatcttcct gggtgaggcg ctgaacgagg cccgatcctt ccgggtctcc gggtaggact
     3601 cgaggcctcc cggcccaacc gaagtacttc atcgaggcgc agacgcccgt ctcttcgagc
     3661 gtgacctcgt agcggaggtc gagtccgatg tcggagggcg cccaaccgcc ggggcgatgg
     3721 cagtgcgcgc agtttgcgtc gaggtaggct cgcgcgcgct cctcgaggct cccttctcct
     3781 aggcgcggat tcgccatccg gggctccatg gcggggtcga agtcttcgcc cgcatcgagc
     3841 tccaggagat cgatggaagc cattgcacgc agctggttgg cgaccacgcc tccgtaatcg
     3901 tgctcgcggt tgagctgcgc ggtcttcggt ccgagcacct cgttgatcgc cccgccatga
     3961 caggtgatgc aagcatcgta atcggggtac tgatattcga tcacgctgac cgcgtttccg
     4021 tcttggatgg agtactccac ggtctttgcc tcatcgagac gctctgcgtc cgttccgtcc
     4081 tcgttccatt gatacgaggt gtactcccag accccgtccc gcagcaccat gaagcgtgtc
     4141 tccacggggc gccggctcga gggatccccc cgttcgaact cgaatccgaa caccttgatc
     4201 aagatgctcc cttcgggaaa cgtccaagcg ccgccgtctt cgattcggat tcgcttcggg
     4261 ctcggcacga ccatgaagcg cggcttgaat gcgccgtcgg tccagagggc ggagttgacc
     4321 tcgtacggga tcaggtcggg tcccggctcg aaggacgcga ggtcctgaaa acaaccggtt
     4381 tcggagaggc gttgcggcat gcctgcagat aaatcggaca agcaggtctg cacctcgggg
     4441 cggggctcga gcgcagaccc agagggctcc tcaccgccgc aggctatcag cgagagaagc
     4501 gccaaacaga ccccgaagct gaggcaccgc atgattcagg tctagcggaa aatcgagcgg
     4561 cgcccaagct ccccgcggcg ctcattttgc gcgcgctgag gcctcgactt ctttccagcg
     4621 aaaaggagag agaatctgat cccaatagcc cgtcggaaag cgcggtagat ccccgatgcc
     4681 gatctcggtt ggcgggtttc gatcctgtgg gaggaaccgg gtctggaaga cgatgtccca
     4741 gaggatgacg tttgcgccgt agtttgtatt ggcctcttcc agatgcttcg aatgatgcca
     4801 gcggtgcagc tcggccatgc tgaagaacca gttcagcggg cccagccgaa tgtcgacgtt
     4861 gcaatgctgg aacatgccat gaatggccgt gaagagcgtg tggagtgcga tgatgcgcat
     4921 gtcggcgccg aggagaacca atggagtcac tgccaagaac tgctgcgcaa acaagtccat
     4981 gggatgaaat cgcgccgcat tgaaccagta gagccgcggc gcgctgtggt gcgcagcgtg
     5041 gaagcgccag aggaatgggt tctcgtgcat ccatcgatga atccagtagg tcccgagctc
     5101 cccgaccagc agcgcgagaa gcagctgtgc gagcaacggc cagcccgaag gccagagctt
     5161 catcccgagt gcgctcgata gccctgcccc ggcgagcgtg aatcctccga cgaagaccac
     5221 ggtgtagagc tccggaatcc agaaattcac gaggttgtgg atcaggtcgg ttcggacatc
     5281 tccatggctg atcaaccagt ccttctggta tggatgcact cgttcgagca cgagcagcag
     5341 cagcacgacc gcgcccgtga gtcccatggc ggtggcgacg gggttccagc cctgccctac
     5401 gccataaagg ccgagtccca tcacgcctcc gaagatgagc ggaaacgttc cgtacgtgag
     5461 tgcagcagcg atgggcttgg gcgtgtccat ggtgatggcg atcggcgcta ggtgatcgag
     5521 aaccctccgt cgacgagcag ggtctgcccg gtgatgaacc gcgccgacga gctggcgagg
     5581 aacaacgcca ccggcgcgat gtcctccggc gcgccccatc gccgtaaggg cgttctgtcg
     5641 atgaatggct tttcgagtgg ctccacgcct ttcatgatct tggtcatgtt gctctcgacg
     5701 aggccgggcg cgatcgcgtt gactcgaatc ccctcagcag cccaactggc agcgaggttc
     5761 ttggtcatct ggacggttgc agccttgccg gctccgtagc cgggcacgat tggcacggca
     5821 aagaacgcag ccatcgaggc gaagttgatg acgctggccc cgccttcgag cgtgctcgcc
     5881 gcgagcttgt cgcgacagcc caccgcgagc ctgtacgcgc caaagagatt gatcgccaca
     5941 gattcctcga agacgtcggg tgcagcctcg tgcttgccac cgggaaagtt gccgcctgcg
     6001 ttgttgacga ggatgtcgag gcggtcgagc gaagcggcca gggactcgat ccccgccttg
     6061 tcggtgagct ggagcggatg gtagtcgtag ctcgacaggt cgtcgtcgta ctcactggcc
     6121 gatgcacggg ttccggtgat cgccacctcg gcgcccgcaa cggcgaacgc cttggcgatg
     6181 gccctgccga tcccgttgga gccgcccgtc accaacacct tggcgccaga aaaatcgaat
     6241 gtcgcttcgt tgctcatcag gcgattccct cgtcgatgtc caccatgact ttgcctccgg
     6301 cacccggatc ggtcgcgact tcgatcgcct cgccgatgcg cgcgagcgga aagcggtggg
     6361 tgatcatggg cgaaaggtcc catcggctga ggagcgcgac cgcatcgctg aagtcggcgg
     6421 ggtactccat ggctccacgg atcgtcatct gcttcatcat cacccagaga aagctcacgt
     6481 tgaccgattg ctcgtggagc gcgaccaccg tgaggctcga ttccgctttg gcgttctgga
     6541 gcacctcggt gatgacagag cccgctccgg acgcttcgat gtaggcgtcg gtgcccgcca
     6601 tcggcgcacc catcagtgga gcctcgccgt ggagcttctt gagctctacc caggggttct
     6661 gctcagaggc gaggatcgta gcccgagccc cgagcgctcg cgccaactcg agccgcctgg
     6721 gagacaaatc gatcgccacg acgtcgtcga ttccgcgatc ccgaagcgtg gcgatcgcgg
     6781 aaagcccgat cggacctgcg ccaaaaatgg cgactttgtc tccggcagac acacccgatc
     6841 gattgaccgc gttcatgccg accccgagcg gctccgaaag cgcggcaagg tgcatcggca
     6901 agtcgtcggg gatcggaaag aggcaacccc catccgccgc attgcgaacc agcaatcggt
     6961 gcgtgaatcc tccctccggc ccgccattgc cgatcatgtt cccagatgcc gtcgggttca
     7021 gcacgacgcg agcgccaggc ctgagatcgg tcacttccgc gccgaccgtt tcgatcacgc
     7081 ccgctagctc gtgcccaagc ggcatcatgc ggccaagccc tcccatccgg acgtagcgaa
     7141 cgtccgttcc acacacaccg catgcggcca cgcgcaccac cgcgtcgcga ggaccggcct
     7201 cgggtgggct gatttcgtcg agctcgactt tgccggggcc gtggatcttg acctgttgca
     7261 tcatacctcc gcctggacgg gaaaacgttc gtagtagaat tcgaagcgct cgcgaagcgc
     7321 gttcggatcg actccgaaat caccacgcag atcgtagacg atgcggccgt gttttccgcg
     7381 cggattctcg gtcatgtatt gatcgagctc ggccctcgcc tcatcgttca tgggcaggtt
     7441 cgccaactgg tagattcgct ccaccatcgc cacgtcatcg gacatgaatt cgtgaaacag
     7501 gacgtcgatg ctccgagact ccggcaagag cgcacggtcg cgcacggccg ctctcaacaa
     7561 ctgctccacg cggtcaatcc aataagcggc cactgccgga gggtcgaccc gtgtccggcg
     7621 cagtcggtcg ccgtaagtca gcattgtgat ggccgacgca accaccgaaa cgggatcccg
     7681 gtgcgtgagc gccacggtgg catccgggaa cgtttcgacg agaggcccga gctgctcgag
     7741 atgctgcggt gatttgagta cccaccgatt cggtccatgc atccactgca gagctttcag
     7801 cacgttcttc atgtactggt agtgtggacg ttggtcgtga gccaaatagt agtcgcgcca
     7861 gcgcggaacg agtgccagcc actcgggttg gtacgacgag aaatccagcc ccgcgagctc
     7921 gatctcctcg tgaatgtggt cgggcgcgag gtcgtgcatg tttttgagca gaggtgcgat
     7981 ctggcccagc atctgatact cgctcgcaca cctgaggtac cgcgggtccc tctccttcgg
     8041 gccaaatggc acgctcggat ccggaatcgg ctcgaggctc tcccaatacg ggagcgaacg
     8101 gagccggcta tcggccgcga tcaggttcag caagtgagtc gtgccggagc gagggagtcc
     8161 ggcgatgatg atcggttcgc ggatctcgat ctcgagtatc tcgggatgtt tcccgatcag
     8221 atcgtgaaag cgcaaacgat tggccgcata acgcaccatg tctccgaaga agcgcgcccg
     8281 ccccatcgga ccgagctcgg aatcctcatc gaccgcttgg caccataccg ccaaacgctc
     8341 ttggaaatcc gcgggtccaa agtcagacag ctgagtgcgc ttctccgcct caccgaggac
     8401 cgcatccacg ctgagctcga cttccacccc cgcaaagcca tcgacgacca tcttttgcac
     8461 gggcgtgagt atcggcgccc tgagatcagt gattcgaatg tcggctgctt gctccgtcac
     8521 gaatcctctt ccctgctctt ggctgcgtgc agcgtcgaac gcagtttggt gagttgtttg
     8581 cgaagccact gcgtgagcca gaaattttcg ctctccgcct cgagctgcac gtgcgcagcg
     8641 atcatgacct cgagcgccgc aacgttcgat ggcgaatccg caagtgccac ttcggcaaga
     8701 tggatggctt ccacgggctc accggcgatc agtctctgct gcgcccgcgc ggcgaccgca
     8761 ttcgcgcccc ctgccaagtc gaccaagtcg cggtgcacgc tgctcggcgg gaccgcgtag
     8821 agctcggtcg tcgagcgatg gtggaaccag cccgcgtagt tctcccagat cgcccgaacg
     8881 ctccaggaaa ccttgccata gccctccccg acctcgagct cggggggaag cctgatctcc
     8941 cgcatcaagg tgtgaatgtc cttgccgtga ttcatgccgc gcaccgtctg gtcgtgcacg
     9001 tattgcactg catcacgaag acggatgagc tcctctcgaa ttgtctcccg acccacgacg
     9061 ggaccgtggt ggcctgtcaa gagcagctcg ggctcgaggg caagcacctt ttcgagcgac
     9121 tcgatgaagc cgagcgcttc gcggtaccga tcgccccgaa cggtcaccag gttcggaaag
     9181 tggccaaaga gcgcgctgaa gaggttgccg gtgaaacaga tcttgtgatc tggcagccag
     9241 acgaccatcg agtctgtcgt ctcacctccc ggcgtcgcga tgagctccat ccgaaccccg
     9301 ccaagctcga gcgagtggcg ctcctcgaag gtgacctcgg gcgttggttt cgactgcgcg
     9361 ggaatgcccc ggccgacgtt ttcctgaatg tgcttccagg cgctcgcgat cgcatcggcg
     9421 aaggcaaagg cgcttcgtga cgctcggaac gcggcgatgc gtgcgtcatc ggcttggtgc
     9481 gcctggttct tcgcctgcgc aacgacttcg gtgccttcat cacggatcag gtcgaccccg
     9541 cccacgtgat ccacgtgacc ctgagtcagc agaatgtagc ggactggact gtcgtcgacg
     9601 gcgtcgaaat tgcgcttgtg gatgggtgct tcgaatccca tccccgtgtt gatgacgacg
     9661 cgcccgtcct gggtcgtgat catgtatgaa ttggagaggc cttgggaaac gtagatgaac
     9721 tcgttgatcc gctgcgccgc gggctgtgac gccgggcgaa tctcgaatcc gcccggccgc
     9781 gaacgatgaa gaggggtgga ggtcatggtc tttctgagga cggaatcgcg aggatagctg
     9841 tgctcaacgc ctcatgctag agtcgcggct cgctcggggt gcccatgaag ggctgagaag
     9901 aacccgtcga acctgatccg gtttgaaccg gcgtagggaa gcgaaccatc ggcttcttcc
     9961 ctacgagagg agcaagatga caagtttcaa gatccctctc cgaggcgccc acaacccatc
    10021 gagcgagagc gcgggcacga caccgggctc gttcgccaac gcgcccgata tcggcggccc
    10081 gcgatctttt tcgtcgcccg atacgcctgg catgccccag ccgagcgaca agaccgcgtg
    10141 ggacttcatg ccggaggatt ggcgcttcga gaacggacac tggacccccc cgagcgggtt
    10201 cgaaccggta acccagctcg agcatgcacg gctcggcacc gtgaccgcgg agatgcgccg
    10261 cgtagcggag cgcgaaaagc acctaagccc ggaacaagta cgcgacgaag tcgctgctgg
    10321 gcggatgatc attccggcca acaaggtcca tctgcggcat gcgctcgatc cgatgtgcat
    10381 cggccgttcc ggcctcacca agatcaacgc gaacatgggc gcgtcgcccg tttcttccgg
    10441 caccgaggag gaagtcgaga agctgcgttg ggcggaacgc tggggcgcag acaccgtgat
    10501 ggacctttcg accggcggag acctcgacgc gacccgcgaa gcgatcatcc ggaacgctcg
    10561 tgtaccgatc ggaaccgtgc ccatttattc gatgatcatc ggacgcagca tcgaagagct
    10621 caccctcgaa gtgatcctcg acacccttcg ccaccaagcg gaacaaggag tcgattactt
    10681 cacgattcac gctggagtcg cgcgcgagca cctgccgttc atccgcaaac gcctgatcgg
    10741 cgtggtgtcg cgcgggggct cgttgctggc caagtggatg ctgcaccatg ggcaagagaa
    10801 cccgatgttc acgcattttg aagcgatttg cgacgtgatg cgcgagtttg acgtttcgtt
    10861 ttcgctcggc gacgggcttc gtccgggcgg gctggccgac gccacagacg atgcgcaact
    10921 aggcgagctc gatcgtcttg gcgagctcac cgagcgcgcc tggcgcaagg ggtgccaggt
    10981 gatggtcgaa ggtccgggac acgttccctt cgaccagatc gagtacaaca tgaagctcca
    11041 gcggcagcgc tgccacggcg ctccgttcta cgtgctcggc ccgttggtga ccgacgtatt
    11101 cccggggtac gaccacatca cgagcgcgat cggcgccaca gccgccggct accacggcgc
    11161 cgcaatgctc tgttacgtga cgcccaagga gcacttgggc ttgcccaagc gagaggacgt
    11221 gaagcagggc tgcatcgcgt acaaaatcgc cgcgcatgcg tcggacgtgg cgctcggtat
    11281 tcctggctca cgagactggg acgacgacct cacacgggct cgggccgcgc tcaactggga
    11341 aaagcacttc gatcttgcgt tcgacagcga cacggcgcgg gcatttcacg acgaggatct
    11401 cgacgtggac acggacttct gcgcgatgtg cggtcacgac tggtgctcgg tgcgcatatc
    11461 gaaggaaatt gtggagttcg agtccggcaa ggcgagtggc ttcgtgcgcg aacgcgtgat
    11521 gaagtcaccc gcactcacac ccgagcaaca ggaaattctc cgagcgagag gccacctgtc
    11581 acccgaagag atccaccggc tcgccggcaa gacccagcac gcagttggag cggcggaaaa
    11641 agcggcctgt cacagcgacc tggtgagcga cgacacccaa gccttgcggg tccagggcga
    11701 aaagctgcta cagttgcgac gtaaggggac tgaaggagcg ccaaccgcat aaggcgcccc
    11761 cgcggaaagg taagaagatg gggaagaaac tgatcctagg cgtgatgatc ggcatcgtgg
    11821 cgactgcatt tggttgctcg aaggagtctg gcggcacgac cgcgtactcc ctcgaagatt
    11881 tcgacttccc gctggtgtcg accgacgtcg caggtggcgc cgagactttc gccgagttct
    11941 gcgaaggttg ccatcccggt ggcgagaaag gcgacggccc caagatctcc ggtgaaggca
    12001 tctccccttc tcagatgcgc tggagggtcc gaagtggcgg tgatgacatg cctgccttcg
    12061 gcccagacaa gatcagcaac gccgatctcg aatcgcttct tgcctatgcg gaaacgctag
    12121 gcgccgtctc tcgctagcgc ctcacactgg gttgggcgcc cggactacag gctggtccta
    12181 gggcaggact tgccgtgcga tctccgctgg gttgtccccg gctctgagcg ctcgcagcca
    12241 aaattggtag ctctagggaa ttccccaagt cgttgtcggt catcgcgcgc cagaagtcgg
    12301 gtctcaacgg ctcgtcgaga cgctcgtttc catcggcgct aaacgatgtg agcgcaccgt
    12361 tggacttgtc gacacgaatc gagaaaccct ctccgctcac ctgaaccgag ctctcgtcct
    12421 cggcgacgac gagcgccggt gcttcggacg ggtcccgagg cgggccaggg atggccgcgg
    12481 gcggagcgaa gtcggcccag gccagctcat gcccagagtt gatcgtcttt gaaacgaaat
    12541 cagaaccgaa gccgcggttc cgcccctggt ggcggcgcca tttcgatcga cggaaggtcg
    12601 acgacggcgc tgcttccagg ctcgagactc ggcagctcga atggcccctc gtcgagcacc
    12661 tcgccgtcga ccgtgaatac gtagtggccg accagccgct cggacaagtc gatatggtct
    12721 aagcggttgg tgatgcgcat ccgtccagtc tcgaaatcaa aatcgtccac ctgcacgggc
    12781 tgccttggtg ttagtctcgt tcagctccgc ccgaaactcg aggcctcgta tctctgaggg
    12841 accaacggat agtggtgatt gaaatagatc ggctcgccat ggcctgacaa ctccacgttc
    12901 gacggaaccg gaatctcctc ccattcggag tcgtcgaagt ctgcggcctc aaatcccccg
    12961 ggacgcccgg cgggcgaatc ggagaatcga aaccgccacg agccattgag cgagatcgaa
    13021 taagcaggtt gggcgacatc ctgtgggaag tcgggggtgc ccgaaacacc ttcaggttca
    13081 acgagcttcc agaggccaca ggtcgctctg tcgctggcgc tgctgctgcc cctgacccta
    13141 cttcgccgac agcctgtaca ccttgatgtt ccggtcggtg cgcttcacgt aggacttcat
    13201 ctgaggaatc gtgtcggcga ttttcggcca gaggatggct ttttccgaat cggtcaagcg
    13261 cgtggccacg acctttttgc tttccgcgcc gagcaagatg ctggcagcgg gattcgaatc
    13321 gaggttgagt gaccaggcgg gatgactggt cccgccgaaa ttgctcgcga cgatgaggac
    13381 gtcgtcgccg tcctcggtgt atccgagctg gacggttcgc gccttgcctg acttgcggcc
    13441 gatcgtggtg agcgtgagct gcgggatcgt caaaggtccc gtgctcgtga aacggccgcc
    13501 gctgacctta tagaggaacg gatcgacctt tgacgcaagc gtctttgcca tccacacgcc
    13561 cactggcgtt accgagaagt tgttgatgaa gtcccgatag ctcatctcgc ctccaaggtt
    13621 gagcacctta gcacgagcgc agcggcttta gagtcccaac cagcttctcg cgtccagggc
    13681 gcgagtgccg cggcgaactt cgaagaagag aatcgcttcg ggcgacccca tgtccagggt
    13741 ccctatctga gtgctcatgc cgacccagtc gcccgtctga acgtcagtgg agccaaggcc
    13801 cccgtatacc gtgtaaaaac tgttgccgtg gtcgatgatc accatcagcc cgtaggcgcc
    13861 gtaattccgc gcaaaggcca ctcggccgtc ggctgctgcg cgcaccggac ctcctgggcg
    13921 cccgtagaac tcgagccctg gcgctccttc gcgggacgct tcgccgattc gcatcgagcc
    13981 ctggaccggt agcgccagct ggccgcgcat ctcgg
//
LOCUS       DASRZF010000412        34167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3751213, whole genome shotgun sequence.
ACCESSION   DASRZF010000412 DASRZF010000000
VERSION     DASRZF010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 34167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34167
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3751213"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VFG22_18225"
     CDS             complement(<1..518)
                     /locus_tag="VFG22_18225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418239.1"
                     /translation="MRALDDATSSLIDLIEVAYDLEISDSTWLPTVVETGAPILDQGL
                     GIYAADFVHPAADGGGAQVTVNSTHLHSLPEDYPERFDVARSLVPPGVYQKVSPPGFA
                     GTWSEMTKDYPEISKACLEALGCGDLLGISASDPNGVGVHINAPLPEASRLTPRVRER
                     WQMVGAHIASAFR"
     gene            complement(562..996)
                     /locus_tag="VFG22_18230"
     CDS             complement(562..996)
                     /locus_tag="VFG22_18230"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET6418240.1"
                     /translation="MNPIKEVRVRDFMMTKPIIFSRETDLLDAIRVLVDNRISGAPVV
                     DSRGNLVGVLTERDFLHAALVAGYHGERGGCIGDYMSAPAEAVNADDSLFDVATRFVQ
                     SKYRRYPVVQDNRVVGVVARSDVLRAIMNAFGRRRSGVRASM"
     gene            complement(1190..2416)
                     /gene="nqrF"
                     /locus_tag="VFG22_18235"
     CDS             complement(1190..2416)
                     /gene="nqrF"
                     /locus_tag="VFG22_18235"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HET6418241.1"
                     /translation="MGLQIVLAVTIFTGIIMLLSALILLARSRLVETGTVHMLVNGDK
                     EFQVAAGVKLIGALANVGLYLPAGCGGKGTCGQCRVKVLDGGGPLLPTEESLIGRLEA
                     AQNVRLGCQVSVRENVRVVIPEEVFGVRKWECTVRSTKGLSTFIKEIVLDLPSGEEVP
                     FRAGGYVQIECPRYRAKFSDFDIEPRFRDEWDRHDLWRFQAGTKTPTTRAYSMANYPD
                     EKGIILLNIGIAVPPPGSKESVPPGVVSSYLFSLKPGDEVTIAGPYGEFYARDTDNEM
                     VFIGGGTGMAPLRSHILDQLKRLKTDRKISFWFGVRSKRDMFYHEEFLRLAAEHPNFT
                     YVVALSDPKKGDDWDGPVGFIHRVAYDRYLGTHDTPEDCEYYMCGPPLMISAVQKMLT
                     DLGVPDENVMFDDFGS"
     gene            complement(2428..3036)
                     /gene="nqrE"
                     /locus_tag="VFG22_18240"
     CDS             complement(2428..3036)
                     /gene="nqrE"
                     /locus_tag="VFG22_18240"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HET6418242.1"
                     /translation="MGGYIELFIEAVFVENLALSFFLGMCTFLAVSKRIQTAFGLGAA
                     VVVVQLITVPTNTLLYHFVLEDGALGWAGLPDLDLSFLGLVTYIGVIAAMVQILEMVL
                     DRYFPPLFHALGIYLPLITVNCAILGGTLFSVQRDYSTSESIVYGIGSGFGWALALIG
                     LAGIREKLKYSDVPAGLQGLGITFIIVGLMSLGFMAFAGIHL"
     gene            complement(3036..3698)
                     /locus_tag="VFG22_18245"
     CDS             complement(3036..3698)
                     /locus_tag="VFG22_18245"
                     /inference="COORDINATES: protein motif:HMM:NF006777.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HET6418243.1"
                     /translation="MSSAAKRALLDPVVDSNPVTLHVLGICSALAVTTSLLPSLYMCL
                     ALTVVAACANAAVSTIRHVLPGSIRIIVQMTIIASLVIVVDESLKAFAFETSRELSVF
                     VGLIITNCIVLGRTEGFASKNPVGLSILDGLGNGFGYSVILILVASTRELLGAGTLLG
                     YTVLPKVADGGWYNPNGMMLLPPSAFFVIGLIIWAVRSWKAEQVEKPEFRIHEVHRTE
                     AL"
     gene            complement(3695..4498)
                     /locus_tag="VFG22_18250"
     CDS             complement(3695..4498)
                     /locus_tag="VFG22_18250"
                     /inference="COORDINATES: protein motif:HMM:NF003749.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HET6418244.1"
                     /translation="MPEPDQSSGAKTETVARTLLVALVVSLVCSSFVASAAVLLKPRQ
                     VANAVLDVRRNILRVAGLYDGGGDVEQLYRSIEPRIIELETGAYVQSADPEAFDPVEA
                     AKDSALSVEVPAEIDVARIGRRAKLARVYLVHGDAGIDKIILPVSGYGLWSTMYGFLA
                     LEADGNTIADITFYEHGETPGLGDFVGKPAWRALWREKRLFDESGELQIQIAKGHVSS
                     DDPMADYRVDGVSGATLTGNGVTNLLEYWLGDHGYGPYLRRIREHGGDL"
     gene            complement(4491..5705)
                     /locus_tag="VFG22_18255"
     CDS             complement(4491..5705)
                     /locus_tag="VFG22_18255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HET6418245.1"
                     /translation="MRPLRKALDKVGPHFANGGRFERFQAIYEMLDTLLYSPPDVSRN
                     APHVRDSIDLKRVMGLVVLGVLPCALVAMWNTGYQANLAIQDLGLPAPDGWRGAVLTA
                     FGVGHDPQDLAAATFYGMLYFLPIYLMTLAAGGVWEVLFAAVRNHEVNEGFFVTSILY
                     ALTLPATTPLWQVALGISFGVVMAKEVFGGTGKNFMNPALAGRAFLYFAYPIEMSGES
                     VWTPPIDVVTGATPLAIAAESGVPGLLDQGVTWMDAFLGTIQGSIGETSTLACLLGGV
                     FILYTGVASWRIVTGVMLGMVATAMLFNGIGSATNPMFAMPWHWHLVLGGFAFGMIFM
                     ATDPVTACVTNPGRWIFGILVGVLTVVIRVVNVAFPEGIMLAILFANIFAPTIDHFVM
                     RANITRRLRRSA"
     gene            complement(5705..7054)
                     /locus_tag="VFG22_18260"
     CDS             complement(5705..7054)
                     /locus_tag="VFG22_18260"
                     /inference="COORDINATES: protein motif:HMM:NF003759.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HET6418246.1"
                     /translation="MRIRIRKGLNIPIAGAPVQRIESGNDVGWVALVAKDYEGLRPRI
                     LVHIGDHVRLGQPLLTEKSDPSVVFASPGAGEVVAIHRGPRRSLQSIVIRLEGDEEQR
                     FQAFERGSLSKLDRADVLDLLLSSGMWTTLRTRPFGNIPAADAAPHSIFVTAMDTNPL
                     SADPSIIIAERAQDFSDGIAVLSRLTDGKVFVCQSPGAQAELPDLESVTAAFFDGPHP
                     AGLPGTHIHFLDPVSDKKSVWHLGYQDVIAIGSLFTTGRLSTERVIALGGPVVRRPRL
                     LRTRLGASTQDLLNGELEATDCRVISGSILGGRRAAGRARYLGPHDLQISVLHEDRSR
                     RFLNWLSPGFGRFSSIRAFTSALRPRSHRFPLTTSQNGSPRAMVPIGNFEKVMPLDIL
                     PAPLLKSLIVHDTETARALGCLELSEEDLALCSFVCCSKYEYGTYLRETLDLIEKRG"
     gene            complement(7183..8496)
                     /locus_tag="VFG22_18265"
     CDS             complement(7183..8496)
                     /locus_tag="VFG22_18265"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET6418247.1"
                     /translation="MKLITSHETTDFDALASCVALQRLFPGSVIGLSGGFAPNVHQYL
                     TLHGDRFRTERSQDLDLAEVDLLMVTDVRRRSRLTHVESVLERAARNPRSVRVLVWDH
                     HPSAPDDLHADEEHVQPVGSVTTLLVEELQARGIEIDREEATLFSLGIHEDTGSLAYA
                     RSSSRDAMALAWLLGQGAQPGLLQRFLRPPLDAVERQLLVRLLEGVRAVPRPGASIGI
                     AALETTEVPAGLARVSTEAFRILPHDALFCLYRMPSGKTQIIGRAEANTVPVGAILQE
                     LGGGGHAGAGSGVVHHASLAEVVMRLEAQVERLSCSPRCVRDLMSSPVRTVTPDMPLR
                     DLAQSLHEWGHTGAPVVDDGKLVGVISRRDVERAEREGRGSLSVKSCMAQNVYSIGPD
                     ELLTEALEQMMTHNIGRVPVLDGQRIIGILSREDVLGHLYEESPC"
     gene            complement(8493..8909)
                     /locus_tag="VFG22_18270"
     CDS             complement(8493..8909)
                     /locus_tag="VFG22_18270"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET6418248.1"
                     /translation="MLAKDIMTENPVTATELMSVAEALGILYELDVRHLPVVRGRELA
                     GIVSDRDLRAFIQGSEDDAIDAVENARSATIGTFMNTDPVRVDPETNVTEILELMLMH
                     RVGAIPVADLDTGDLLGIVSYVDLLRVLQGMLDPGT"
     gene            complement(8943..9794)
                     /locus_tag="VFG22_18275"
     CDS             complement(8943..9794)
                     /locus_tag="VFG22_18275"
                     /inference="COORDINATES: protein motif:HMM:NF025615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiC-like family protein"
                     /protein_id="HET6418249.1"
                     /translation="MGTVQRKMVPREHGAYAELLFPIVTVFLGGAPSTSTWLLATGAI
                     ACFLANEPLLILVGQRGTRTKREESGRAQRALLIFVLVALGSGIAGLLLAPRASQIAV
                     GVPLVLGASLVMLAIQGLERSMVGETLAAMTLSSIAIPLGLSAGLGLTETLAVALIWL
                     VTSLLATAVVRLTVARTRAKTESDQARVRMKRVLLVLVSVLVIAIGVAAPYGVRAGLW
                     VLAAAVPVAVVVLVMAVLQPTARRLRLMGWSLVAANLCSLIAVVTALKIIQDFESVEP
                     TLPFPMF"
     gene            9959..10687
                     /locus_tag="VFG22_18280"
     CDS             9959..10687
                     /locus_tag="VFG22_18280"
                     /inference="COORDINATES: protein motif:HMM:NF024778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET6418250.1"
                     /translation="MWTAVVAGAAAGLASVPHCTAMCGPLAAYACSGRPGVSGQGRYQ
                     LGRFLSYSVLGAIAGTIGGATAMSLPRAWGGALLSWSLALGLGLAALRLWRRPNQPLV
                     TLRTKDEGPQPSVVSRALGGLGRHPFFVGLGTALLPCGALAAAVLIAASSGSALLGSL
                     SMLAFSVVSGVGLVGATFLFERFARRPRPVTSRVLAVALALGSILFVIRPVHALRTGD
                     TAACHAPAGEQAAPDEHKHHAHSQ"
     gene            10741..13092
                     /locus_tag="VFG22_18285"
     CDS             10741..13092
                     /locus_tag="VFG22_18285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HET6418251.1"
                     /translation="MARCVHCQQPLVNDSLAPYCCRGCRAVHGILTRAGLGRYYELRR
                     GPGLPPADMESRRIDHKWLELIEADRSGEAGVKRVELDIQGIHCAACVWLIEELFRRH
                     GGTRTGTMSINPALGRVELRISARFPLSDWVDSVEDLGYLLGPARSTGARASDGLLLR
                     VGICAALAGNVMLFAAAIYLGLREGPIYQLLNELSYAAGALAVLVGGSVFIGSAARAI
                     RRRVLHLDVPIALGIVLAFAGSTYSFFFASGHAAYIDTLTVFVALMLVGRWLQQRMLE
                     RNRAQLLADDGTESLLTREIKDGVVRIVHCIGVQEGDRLLIAPGDLVPVELRLLDKTA
                     TCSLDWIDGESRPRRFGSGETVPAGAFNVGERAFTAIATSDFAASSLVGLLGRDPASR
                     GDGPLATRWWSQLSRYYVIGVITLAVCGIGYWWWRTSNPLFAIEVGTAVLVVTCPCAF
                     GIAAPLAYELVLTQLRRAGVFVRVGDFLDRVRTMTRIVFDKTGTLTTGMLEVVNPRAL
                     ETLSDPEITLLYSMSARSAHPKSVAVRRALEGHRAARFIELEVEELTGYGVSAFENGR
                     LYKLGAPHWAASRSVDDGDVVFSVDAEVIAVLETDEQLRPGARTEIERLESEGYQLAI
                     LSGDTPSRVVSLGVSLGMPEELCIGGQNPEQKAAYVREHEPARTLLVGDGLNDQVAMR
                     EAACSGTPAVNRPFMASRCDFYFVSPGLRPVAQVLHASDVLAHVVRRILAFAIAYNLV
                     AVSLAMAGLMKPWVAAVLMPLSSLVTLAYTVMMLTRKGLSWRS"
     gene            13080..13280
                     /gene="ccoS"
                     /locus_tag="VFG22_18290"
     CDS             13080..13280
                     /gene="ccoS"
                     /locus_tag="VFG22_18290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HET6418252.1"
                     /translation="MEILILLIFVCVVLVGVAVAFFAWTVRQGTFDHADRLALLPLDD
                     EKPMTPTTEREEDEYGNSADRL"
     gene            13255..15600
                     /gene="ccoN"
                     /locus_tag="VFG22_18295"
     CDS             13255..15600
                     /gene="ccoN"
                     /locus_tag="VFG22_18295"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF011053.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HET6418253.1"
                     /translation="METQRIVYNDKVVRQFVWASVLFGIVGMLVGIIIALQLAFWPAN
                     LPPYLSFGRLRPLHTNAVIFAFVGNMLFAGIYYSTQRLAKARLASDILSSIHFWGWQL
                     IIVSAAITLPLGITTAKEYAELEWPIDIAIALIWVVFAVNFFWTLAKRREKHLYVAIW
                     FYIATIITVAVLHIVNSFEIPVGLFKSYSVFSGVQDALVQWWYGHNAVAFFLTTPILG
                     IMYYFLPKAAERPVFSYRLSIVHFWGLVFLYIWAGPHHLLYTALPDWAQSLGMVFSLM
                     LWAPSWGGMLNGLLTLRGAWDKLRADPVLKFFAAGVTFYGMSTFEGPLLSIKSVSGLA
                     HYTDWIIGHVHSGGLGWNGLMAAGMFYWLVPRLYGTKLHSRAAANFHFWIATVGILLY
                     VVSMWVAGITQGLMWRAVSETGGLLYPNFVETLLAIKPMYWARLTGGLLYITGFILMA
                     WNLIKTAKSGAPVDGEIEVAVVTDPRDREIPWPKLLFGQPVMAAIIVMGLLFAMGLFD
                     GFISTVLALVALMWAVAAIAISIRGRDEDKISWHHALEGRAGVFTVLVVIGILVGGVA
                     EIIPLVISVPESIRTTKNVPYTPLELEGRDVFIREGCYTCHSQMIRPFTWETARYGAV
                     STLDDSLFDHPFQWGSRRIGPDLARVGRKYADVWHYKHMINPREISPGSNMPPYPHLA
                     TETIDFANTTVKLRAMRNVGVPYKADQIQTAEARAQAEAASIAAGLSESAGVKVCDTP
                     GEGCELLVNSRLVALIAYLQRLGHVPEGEPLAVSNTTEVAP"
     gene            15597..15782
                     /locus_tag="VFG22_18300"
     CDS             15597..15782
                     /locus_tag="VFG22_18300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418254.1"
                     /translation="MSRFASEFFEQSPVLLYPVLALVLFFIVFLVVIVRVMRMKASEA
                     DLCARIPLEEELEVPHE"
     gene            15775..16380
                     /locus_tag="VFG22_18305"
     CDS             15775..16380
                     /locus_tag="VFG22_18305"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HET6418255.1"
                     /translation="MSEAKRTDEIQGEILHVYDGIEEADNNLPTWWLLTFYGAVVFGL
                     IYWFYYHEYAIGQLQPQKYAEAVAAAEAERSIVTDESLDALALDESAVTAGSQIFATQ
                     CMACHDSKAQGRVGLGPNLTDKYWIHGGAPTSIHATITNGIAAKGMPAWAPILGEEGV
                     RQVVAYVLTLRNTEVPGVEPQGEVWEPGGTLADDAAGSKSP"
     gene            16384..17769
                     /gene="ccoG"
                     /locus_tag="VFG22_18310"
     CDS             16384..17769
                     /gene="ccoG"
                     /locus_tag="VFG22_18310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HET6418256.1"
                     /translation="MTKLPIVEQPASSLRSDGSRNYVHPADVSGRFTRLRYTIFSVLI
                     AIYVVLPFVKIGGHPAVFIDIVNRRFYLFGAVFNAQDFWLVFFLLSGVGLALLIVTTI
                     KGRLWCGYACPHTVFLEGVFRRIERLIEGPRAQRLRRNAEGLTFDKVWRKGLKHAVYI
                     VLALLLSHVFISYFVSLPSVLEMVQRSPAEHPTAFTWVVVMSGILYFNFSWFREQLCL
                     IICPYGRLQSAMTDRDTMVIGYDWNRGEPRGKASNPENGDCVDCKRCVVVCPTGIDIR
                     NGLQMECVGCAACVDACDEIMEKVSRPQGLVRYDSLNGLEGKAKHLGRPRLFFYGGVV
                     ALWLIGAVFAVAQSADFETNVLRLTGAPFSVVDGQVRNSLRVHLVNKTGQKQAFLVVP
                     ETESGIEYIVPQKRVELESLGSTYLPILAILPREQMRPGLKVQLTISMEGRDDRKASR
                     LVEAPFVGPGS"
     gene            complement(17782..18192)
                     /locus_tag="VFG22_18315"
     CDS             complement(17782..18192)
                     /locus_tag="VFG22_18315"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET6418257.1"
                     /translation="MSLIPRLVVAYDFSDPARRAFELTVFMGRQLKAEVHVVTVLDSK
                     ESEASSSGKEIEDLRQLLELRTSATVQSEDLQIRCHAVTGHPADAIVKVAREIDAPLI
                     ICGTTGKGAVARTLLGSVSHELVHKSGAIVMSVP"
     gene            complement(18196..18675)
                     /locus_tag="VFG22_18320"
     CDS             complement(18196..18675)
                     /locus_tag="VFG22_18320"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET6418258.1"
                     /translation="MDKPQTILVGTDFSDISDEALRAAALYAKTFGSRVLVTHVFDPT
                     PNVPPVVWSRPDLLERSIRVEIEEAIRETLNGKTAELLKGVPEVELSVIQHPSPGRAL
                     VEMAEDEAVDLLILGSHGRSGLSRAFLGSVAERVVRHAPCPVLVVRGRCDNKPKDGE"
     gene            18875..19336
                     /locus_tag="VFG22_18325"
     CDS             18875..19336
                     /locus_tag="VFG22_18325"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HET6418259.1"
                     /translation="MSDEPLMPSEVRRRVLSQHREIEQMLAELETGVTKLNDGSVDAG
                     QVKRAAYALRGILELHMSFEETHMAPAINDADGFGPERVRHLHAEHADQRAEMDRLIG
                     SIREAESRAELTKSVVKLAEMLRIDIEEEEREYVNEKLLSDHLIPSDPFGG"
     gene            19368..20261
                     /locus_tag="VFG22_18330"
     CDS             19368..20261
                     /locus_tag="VFG22_18330"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET6418260.1"
                     /translation="MQIKQILVPTDFSENAQHAVDYATELAKKCSAKLHLLHTPVVPT
                     YLLMDLSYSPGPEAVTRILNDAQEALDRQAKSLAGSGVELFTAIREGTIHEVIRDYAK
                     EHDVDLVVIGTHGRTGVSKLMYGSVTERVIKTVHTPIIVIPPVGGKLPSSIVIAYDFS
                     GPSKRAAEIAHAIHDVFHGPLHMVHCYLDVWGEYTDRGAVVGEAAEKRREALHLGLEE
                     MLQTEAKELFSEDPQGVQTHLLTGDPAEGLLSVAGKTGATLICAGTTGKSGIERLLIG
                     SVARRLLHDSKVPLLLTHDDG"
     gene            20311..21114
                     /locus_tag="VFG22_18335"
     CDS             20311..21114
                     /locus_tag="VFG22_18335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418261.1"
                     /translation="MLGRVVVMGVVAALLSGCECEPKRLPQASETPAGFDVVMKEHGQ
                     LSIISRNALIRGELPLAQQSMRKLLFFMEHVPFPEPGKEYARITKELATEVKEASDME
                     EACMAFSRLSHACGQCHHALDRGPPIKLEPAAGGDDLRMHMRRHYWAIERMWEALLSD
                     SPTAFQVAAGVLAESPLHGSHQPDGDNPPGTTRLEYEVHDLAFAAAVEGRISEDEYVP
                     KPGELLEDAPTTLGQAEIFGRLLFACEQCHALVDVKPALTPATNRGAVP"
     gene            21114..21422
                     /locus_tag="VFG22_18340"
     CDS             21114..21422
                     /locus_tag="VFG22_18340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418262.1"
                     /translation="MLQHRKQTLRAAMVLLLLAAACASGRVSGGQGTEMAQEEAYEPS
                     NAANPPTGNTDFTSGPIKAITPPPVLQVDLERRRPPTGPPIEPEPEPQSNPETQNEPR
                     "
     gene            21432..22700
                     /locus_tag="VFG22_18345"
     CDS             21432..22700
                     /locus_tag="VFG22_18345"
                     /inference="COORDINATES: protein motif:HMM:NF014594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HET6418263.1"
                     /translation="MSKLCTAEEAVNHVNSNQRVFLHGGVATPTRLVDALVRDANRLR
                     DVELIHLHTSGDGAYARPEYERSFRVANLFVGENMRHQLDGDRVDYLPCFLSEIPQLF
                     RSGRRALDIALVQVSPPDKRGYCSLGVSVDVARAAVDSARIVIAQVNRHMPRVHGDGF
                     VHIDQLDWLVEHDEPLPEVPARPPTPEEKAIGHHTAELVEDGATLQMGIGAVPDAVLA
                     SLKGHRHLGVHTEMWSDGLLPLLECGAVDNSQKVVHPGRTVSGFVVGTRRLYDYIDDN
                     PTVTQYDIGYVNRVNVINRNPKVTAINSAVEIDLTGQVCADSIGHRVISGVGGQMDFI
                     RGAMTSQGGKPIIALTSRTPKGIPRIVPTLRPGAGVVTTRAHVQFVITENGAVDLYGK
                     TLNERAQLLISIAHPDDQEGLERAWHDARA"
     gene            22966..24876
                     /locus_tag="VFG22_18350"
     CDS             22966..24876
                     /locus_tag="VFG22_18350"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HET6418264.1"
                     /translation="MDALPAAAITPDAEWLSVNAGGSFAMGTIDRCPRRKYHGLLTVR
                     EPGVGEPLNMVAEVEEWFRIGGERIALHSYNWGTAIEPHGIDYLSRFEPQPRWTYELK
                     GLSVTRELWLENGADAVWIRYSIEGLTEAVDLRLRPLIRCRPVHELTFANPFMNGELD
                     IEDGKSLRVVMHPYKGVPALIASLHGAPGVFRADGHWYEGVHYEWERERGYDDAEDLF
                     TPGEFRLQLEHDGAFCIRIGTTPAASHEPQVLSSVVDSRRSFLKELDDAAEAYLVELA
                     GGRTGIIAGYPWFGEWSRDALIALPGLCLARGQVDKALAILESLADRRENGLIPNIPQ
                     SGDVPSNGESIDASLLFIRAVALTAKRTRSRRIAALQEVSFDLIEQIAAGADPRVHVD
                     PQGFLFVDHGPWALTWMDALVDGWPVTPRQGYAVDLNALWLSGLGAALRWAKKLRPEF
                     IEKWSETAKKLSDHFEEKFWLQDQGFLTDTHDGNHPDGQLRPNQLWALSLPGVPIDKE
                     RKKRALQAVREKLLTPVGLRTLSPDDPAYRPFYRGGQTERDRAYHQGTVWPWLLGIYA
                     DAVEAIEGSDAAVEEMMPVLAALSAHLRTEGCIGQIAEVFDGDAPHRPGGTPAQAWSV
                     SEVLRVAKMAGP"
     gene            24873..26357
                     /locus_tag="VFG22_18355"
     CDS             24873..26357
                     /locus_tag="VFG22_18355"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6418265.1"
                     /translation="MKILMLGWEYPPHISGGLGTACEGLTTAVASRGIEIDFLVPRLY
                     GEEDAPHMRLLGAASADPMVQDESPPPVATMSHLAELIAAEPALATDPIFVAAVEAAS
                     KGMPQDSAGEIRTIRVPAMLKPYLDQATYRELITSGAISEIALSQIAATQIAHPVPDP
                     PGGVTRLQHAHVPGGDLPLAEQPATGAHYGHDLFAEVARYAMAATRRVAGLKYDLVHA
                     HDWMTFPAGIEIARRAGVPLLVHVHSLEYDRAGRGADRDIVSVERQGLEQATRVIAVS
                     YYTRSLINRVHGTPLDKISVVHNGVYERETVEAYTEQRSSKGPVVLFLGRVTFQKGPE
                     YFVQAAARVLEHVPNAIFVMAGSGDMLPRMKALVEELGITKSFRFPGFVRGAELERTF
                     STADVYVMPSVSEPFGIAPLEAMSYDRPVIVSKQSGVSEVLQNALKVDFWDVDKLASQ
                     IVALLELPELRRVIVERAREEIRHIHWDAAAEKLVPIYESVARG"
     gene            26366..27568
                     /locus_tag="VFG22_18360"
     CDS             26366..27568
                     /locus_tag="VFG22_18360"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HET6418266.1"
                     /translation="MPSVCFYFEVHQPYRLKPYGALQVGRDHEYFDDKLNAEVLRKVA
                     RKCYLPANESMLRLIDRTDGRFRVSYAVTGVAIEQMKLFAPEVMDSFVRLAETGAVEF
                     VAETYYHSLSALYDKDEYREQIELEMKLIKSEFGQTPRVFRNTELIYDDGIGQFISDL
                     GFDAILVEGADDVLDWRSPNFVYRVTDRKTRLLPKNYKLSDDIAFRFSNRGWREFPLT
                     AEKFAGWIHGHTGAGDVINLFMDYETFGEHQWKETGIFEFLELLPELIFTHPHWDILT
                     PSQAIDRYPAIGELPFHRTVSWADEARDISAWQGNAMQQRALREIYALGPAIRRRNNS
                     HLMALWRRLQTSDHFYYMATKSATDAAVHDYFSPYDGPYDAFIHYMNVMKDLKLSVLA
                     PAEKQIRL"
     gene            27565..31821
                     /gene="glgP"
                     /locus_tag="VFG22_18365"
     CDS             27565..31821
                     /gene="glgP"
                     /locus_tag="VFG22_18365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HET6418267.1"
                     /translation="MSKAVFFEISWEVCNMVGGIHTVLASRVPEVQQRHGEDRYVTIG
                     PDVPRTEGVAPEFRPDIWDPELAESLKDHEVSVLMGRWLVPGEPRCLLVNQSRLYERK
                     DEILGRYWEDYGLDSLFGAWDYYDPVLFAHGAGMIIEHIRDRFLLPARQRAIVQAHEW
                     MSAGAILHLKTAAPEIGTVFTTHATMLGRSLAGRRADPNFYKSLADVDPELAAKELNV
                     SSKHSMETVAAREADVFTTVSEITALECKYLLDRSPDVILPNGFGAKPVAPELRQRAR
                     EELFKLAELTTGDTYDRDKTLIFALAGRYEYINKGVDVYLDAATALAQDLESRDGKRV
                     IVYAMLPAGHAEPKRQLWDRAHGASAGAPLRCTHDLVDEANDPITNRLNALGLNNNPG
                     ARVHVVHVPIYLDGTDPLIRQKYWDLLPGADLGVFPSFYEPWGYTPLEAVAFGVPAIT
                     TDRAGFGRWVAAQGDGNRTGVRVLKREGVLTSEVTKALQDAMLEFIDLAQADRDSLGE
                     ACLRTAALTDWSHFMEHYEEAHRLALEAGAARRKGLPMDRLSAPSMPALSSDSSGVFG
                     LFVKPPVKEGEEAAPYTRSFMVTNALPPELQGLQGIASNLWWRWHPEVASLFKRIDPA
                     LWFKIEENPHALLDQVKPERLMDLALNEGYVADVARLHREMVESTETQDPRIAYFCME
                     FGLAGFLKLYSGGLGILAGDHLKAASDLNLPLCAVGLAYQDGYFRQIIDRDGHQEDLG
                     DTNDFGSPPYEQVMTGQYAPLRVTVPLPTGPVQVQAWQLKVGRVPLFLLDTNVPENRP
                     EDRAITNRLYGGDAAHRLRQEMILGLGGFQVLTELDIDPDVFHMNEGHSAFLIIARAA
                     HLIQRHGLRSQEALEYIRNTTIFTTHTPVPAGHDHFPEEMVRPYLTRFEHVLRMDWPR
                     LMAMGSTPSGAQNKFGTTALAVRNAGRINGVSAKHGEVAREMFLQFYPEYENANDVPC
                     TSITNGAHVSTWLAPRWQRLMEREMGHDWRSEIEDPYRWEWLRNHDASEIWSIHRELR
                     ADYVDWLKGHLTETWTRRREDLSLLQDILSRLDEDRMFIGFARRFAPYKRADLLLTEP
                     ARLAKLLNGNPGATIIYAGKAHPADGHGQALLQRVYQATKHPDLAGRVIFMEGYSIDS
                     ARRMVAGCDVWLNTPTRPLEASGTSGMKAAMNGCLNLSVDDGWWLEGYEENNGWVIDL
                     PATSIQDPSAYDRAVTMALLEGEILPTYHDRNETGVPIAWVDMMKASMASIIPKFSAG
                     RMVTDYADNFYEPAMKDAVALRGSNYRPLFALAELAEAIRLRWPEIAMDSVRFGGLGS
                     DELLVGTVVSAELSLHHPGLDARDLDVEAVVTFGSRLHDLSSRIVIPFETDGSDDRST
                     WRGAFTVRASGGHELRFRVRPKDRSPLRPAALGMHNQKWL"
     gene            complement(32096..32860)
                     /locus_tag="VFG22_18370"
     CDS             complement(32096..32860)
                     /locus_tag="VFG22_18370"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET6418268.1"
                     /translation="MANFEMPVSEYMSTPVETIGIGESLIAANGLFSEQGVSALAVVN
                     AEGELKGVVSRTDILHSAVFTQGETFRVPDRSVDELMQSPAIQVSPDAPLTEAAKLML
                     KHRVHRIFVTVGGKPEGVVSTRDLMRAVREKRIKTPISEFASGSVVKVKADDPIAMAV
                     DRLDLSNKHGLVVVEGKFPVGVFDQASALESRRLPPNTAVEHAMNVRILILPEGMALG
                     RAAEQALGMNVRRILIENDHGITGIIGGLDFARAIV"
     gene            complement(32906..33355)
                     /locus_tag="VFG22_18375"
     CDS             complement(32906..33355)
                     /locus_tag="VFG22_18375"
                     /inference="COORDINATES: protein motif:HMM:NF037928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HET6418269.1"
                     /translation="MFKTWVVVAHDAGARFFESDGAGQGLALVEEIEHSAGRARDSEM
                     ASDRPGRAFQKNSGDPRRSSMGQSESPHDRAVSDFARAVAAKLEHGRVTNRYTQLVLV
                     APPRFLGLLRSSLDGPTSQLVVGSLDKDFAAIEASELVERLSDVIAL"
     gene            complement(33378..33713)
                     /locus_tag="VFG22_18380"
     CDS             complement(33378..33713)
                     /locus_tag="VFG22_18380"
                     /inference="COORDINATES: protein motif:HMM:NF036463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5335 family protein"
                     /protein_id="HET6418270.1"
                     /translation="MTTRKLERSTWQKYFDDVAKRLPSMRVGVSVLGDEIGVQLQSEN
                     SALIGISYDSNDQVFEVATPGISHRVPSPKEIYVREQSGMLTSVEVVGEDDSKQIIEL
                     RPLPSLPAS"
     gene            33915..>34167
                     /locus_tag="VFG22_18385"
     CDS             33915..>34167
                     /locus_tag="VFG22_18385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418271.1"
                     /translation="MGNLKADLKAAGFELIETHISWVFLGASDVFKVKRPVNLGFLDF
                     TSLRQRREACDAELRLNRRLAADVYLDVVPITRDELGIHA"
BASE COUNT         6677 a        10642 c        10470 g         6378 t
ORIGIN      
        1 cgaaaagccg acgcgatgtg cgcgccgacc atctgccagc gctcacgtac cctaggcgtt
       61 agcctcgaag cctcgggcaa cggtgcgttg atatgaacgc caacaccatt ggggtcggat
      121 gcggatatgc caagaagatc cccgcagccc agagcttcga gacaggcctt cgatatctcc
      181 gggtagtcct tcgtcatctc gctccaggtg cccgcgaagc cgggaggtga caccttctga
      241 tagacgcccg gcggaaccag gcttctagcg acgtcgaatc gctcgggata atcctctgga
      301 agagaatgca agtgcgtgct gttgactgtc acctgcgcgc ctccaccgtc ggcagctgga
      361 tgcacgaaat ccgccgcata gattccgaga ccctgatcga ggatcggcgc tccggtctcg
      421 acgaccgtcg gcagccaagt cgaatctgat atctcgaggt cgtaagcgac ttcgatcagg
      481 tcgatcaggc tcgatgtggc gtcgtccaag gctctcatca gaccttccca ggatctggcg
      541 aagccctcca tacgtaattg atcacattga agcgcgcaca cccgagcggc gacgtccgaa
      601 agcgttcatg atcgctcgca agacgtcgct ccgcgcgacc actccaacca cgcggttgtc
      661 ctggacaacg ggataacggc gatacttgct ctgtacgaag cgggtggcga cgtcgaagag
      721 actgtcgtcg gcgttcacgg cctctgccgg tgcgctcatg tagtcgccaa tgcagccgcc
      781 gcgttcgccg tgatagcccg cgaccagcgc ggcgtgcagg aaatctcgct ccgtgagcac
      841 gcccaccaga ttgccgcggg aatcgacgac cggcgcgcct gagattcgat tgtccaccaa
      901 gacgcgaatc gcatcgagca gatcggtttc gcggctgaag atgatcggct tggtcatcat
      961 gaaatcccgc acccgtactt ctttgatcgg attcatgctg caggcttcct cgggcattcc
     1021 gagcgttcga tcgccgaccg accgcaggcc ccgcagccct tcccatcggg gttgttcaga
     1081 ccgccgcagg tgccgcggat gccaccacga ccagcgagca ggccgatgct caggccagtc
     1141 aagaccaaaa caaacactag gaagctcaat aggtacagca gcatggtcgt caactcccga
     1201 agtcgtcgaa catgacgttc tcgtcgggca cgcctaggtc cgttagcatc ttttgaacgg
     1261 cagagatcat caaagggggg ccgcacatgt agtactcgca atcctcgggc gtgtcatgag
     1321 taccaagata tctgtcatag gccactcgat ggatgaagcc gacgggtcca tcccagtcgt
     1381 cgcccttctt cggatcggag agcgcgacca cataggtgaa gttcggatgc tcagcggcga
     1441 gacgcaggaa ttcctcgtgg tagaacatgt cacgcttact gcgaacaccg aaccagaagg
     1501 aaatcttacg gtcggtcttc aagcgtttga gctgatcgag gatgtgcgat cgaagcggag
     1561 ccatgcccgt gccgccgccg atgaacacca tttcgttgtc ggtgtcccta gcgtaaaatt
     1621 caccgtatgg ccccgcaatc gtgacctcgt caccaggctt gaggctgaag agatacgacg
     1681 agacgacgcc cggtggcaca ctctctttcg agccgggcgg tggtacggcg atgccaatgt
     1741 tgagcagaat gatgcccttc tcatcggggt agttggccat cgagtaggca cgagtcgtgg
     1801 gggtcttggt tccggcctgg aatcgccata ggtcgtgacg gtcccattca tcgcggaatc
     1861 gcggctcgat gtcgaaatcc gagaacttcg cccggtagcg tgggcactcg atctggacgt
     1921 atccgcccgc gcgaaaggga acttcttcgc ccgaagggag atcgaggacg atctctttga
     1981 tgaaggtgct gaggcccttg gtggatcgaa ccgtgcactc ccacttgcga acaccgaaga
     2041 cctcctcggg aatgacgacg cgtacgttct cacgaacgga gacctgacag ccgagcctca
     2101 cgttttgggc cgcctcgagc ctgccaatca acgactcctc ggtcggaagc agcggtcctc
     2161 caccgtcgag caccttcacg cggcactgac cgcaggtgcc cttccctcca cagccggcgg
     2221 gcaagtacag acccacgttg gctaacgcac cgatcagctt caccccggct gccacttgga
     2281 actctttatc gccgttgacg agcatgtgga cggttccggt ctcgacgagc ctcgaacggg
     2341 cgagcaggat cagcgccgac aggagcatga tgatgcccgt gaagatcgtg acggccagga
     2401 caatctgcaa acccaccaac cgctccttta gagatgaatc ccggcgaacg ccatgaagcc
     2461 cagcgacatc aggccgacga tgatgaaggt gatgccgagg ccttgaagtc ctgcaggcac
     2521 gtcgctgtac ttgagcttct cacggatccc ggcgagccca atcaacgcca gggcccagcc
     2581 gaagccactc ccgatcccgt agacgatgct ttcggaagtg ctgtagtcgc gctgaaccga
     2641 gaagagcgta ccgcccaaaa tggcgcagtt cacggtgatc aacggcaagt agatgcccag
     2701 cgcgtgaaag agcggcggga aataacgatc gagcaccatt tcgaggattt gcaccatggc
     2761 cgcgatcaca ccgatgtagg tgaccaggcc caggaagctg aggtccaggt ctggaagccc
     2821 ggcccagccg agagcgccat cctcgagcac gaagtgatag agcaaggtgt tggtcggcac
     2881 cgtgatcaac tgcacgacga cgaccgcggc tccgagcccg aaggcggtct ggatgcgctt
     2941 cgaaaccgcg aggaacgtgc acattccgag aaagaacgag agcgcgaggt tctcgacgaa
     3001 gacagcctcg atgaagagct cgatgtaacc gcccatcaga gcgcctccgt tcgatgaact
     3061 tcgtgaatgc gaaactccgg cttttcgacc tgctcggcct tccaactgcg cacggcccaa
     3121 atgatgagtc cgatgacgaa aaacgcgctc ggaggcagga gcatcatccc gtttggattg
     3181 taccagccgc cgtcggcgac cttggggagg accgtgtaac cgaggagcgt cccggcgccg
     3241 agcaattctc gcgtgctggc gacgaggatc aaaatgacgc tgtagccgaa gccgttcccc
     3301 aggccgtcga gaatactgag gccaactgga ttcttcgaag cgaagccctc ggtgcgcccg
     3361 aggacgatgc aattggtgat gatcaggccg acgaacaccg agagctcacg gctggtctcg
     3421 aacgcaaagg ccttgagcga ctcgtcgacc acgatgacca gcgacgcgat gatcgtcatt
     3481 tgtacgatga tccgaatgct gccgggcaaa acgtgacgaa tcgtgctcac cgcggcgttg
     3541 gcacaggccg caacgacggt gagcgcaagg cacatgtaga gggacgggag caacgacgtg
     3601 gtcaccgcga gcgccgagca gatgccgagc acgtggagcg tgaccgggtt gctgtcgacg
     3661 acgggatcga gaagcgcgcg ctttgcagcc gagctcatag gtctcctccg tgttcgcgga
     3721 tccttcgaag gtagggtccg taaccgtgat cgcctagcca atactccagg aggttcgtga
     3781 ctccgttccc ggtaagcgtg gctccggata ccccgtccac gcgatagtcg gccatcgggt
     3841 cgtcggatga cacatgaccc ttggcgatct ggatctggag ctccccggat tcgtcgaaaa
     3901 ggcgcttctc gcgccaaagc gcccgccacg caggctttcc gacgaaatct ccgagacccg
     3961 gcgtttcgcc gtgttcgtag aaggtgatgt cggcgatcgt gttgccgtcg gcttccagtg
     4021 ccaaaaagcc gtacatcgtc gaccagagtc cgtagcccga aacgggcaaa atgatcttgt
     4081 cgatgccagc gtcgccatgg acgagataga cccgcgcaag cttcgcacgg cgcccaatac
     4141 gggcgacgtc gatttcggca ggcacctcga cacttagggc cgaatccttg gccgcctcga
     4201 ctgggtcgaa ggcttcgggg tcggcgctct gcacgtaggc gccggtctcg agctcgatga
     4261 ttcgcggctc gatgcttcga tagagctgtt ctacgtcacc gccgccatcg tagagcccgg
     4321 cgactcggag aatgttgcgg cgcacgtcca gcactgcatt ggcgacttgg cgcggtttga
     4381 gcagcaccgc ggcagatgcg acgaatgacg aacagaccaa gctcacgacg agcgccacga
     4441 gcaaggtccg cgcaaccgtc tccgtcttgg caccggagct ttgatcgggc tcaggcactt
     4501 cttctcagcc tcctagtgat gttggcccgc atgacgaagt gatcgatcgt aggcgcaaag
     4561 atgttggcaa agaggatagc cagcatgatc ccctcgggaa atgcaacgtt cacgacacga
     4621 atgaccacgg tgaggacccc gacgagaatc ccaaaaatcc atcgacccgg attcgtgacg
     4681 caggccgtga ccggatcggt agccatgaag atcattccaa aggcgaagcc gcccagcacc
     4741 aaatgccagt gccacggcat ggcgaacatg ggattggtcg ccgagccgat cccgttgaac
     4801 agcatcgcgg tggccaccat gccaagcatg accccggtca cgatcctcca cgaggccaca
     4861 cctgtgtaga ggatgaagac gccaccgaga aggcaggcga gagtcgaggt ctcgccaatc
     4921 gacccctgga tcgtacccaa gaaggcatcc atccaggtca caccttgatc aagaaggccg
     4981 gggaccccgc tttcggccgc gatcgcaagc ggcgtcgcgc ctgtgaccac gtcgatcgga
     5041 ggcgtccata cggactcgcc cgacatctcg atcgggtacg cgaagtacag gaaagctcgc
     5101 cccgccagcg cagggttcat gaagtttttc ccggtgccgc cgaagacctc cttggccatg
     5161 acgactccga acgaaatccc gagagccacc tgccaaagcg gggtggtcgc tgggagtgtc
     5221 agcgcgtata ggatcgacgt cacgaaaaag ccttcgttga cctcgtggtt tcgaaccgcc
     5281 gcgaacaaga cctcccacac cccacctgca gcgagcgtca tcagatagat cggcaagaag
     5341 tacagcatgc cgtagaacgt tgccgccgcg aggtcctggg gatcgtgacc gacgccgaac
     5401 gcggtcagca cggccccacg ccagccgtca ggagctggga gtccgagatc ttgaatcgca
     5461 agattcgcct ggtagccggt gttccacatc gccaccaggg cgcagggcaa gaccccgagt
     5521 acgaccaggc ccatgacgcg cttgagatcg atcgagtcgc ggacatgagg cgcatttcgc
     5581 gacacgtcgg gcggcgagta caggagcgtg tcgagcatct cgtagatcgc ctgaaagcgc
     5641 tcgaagcgtc cgccattcgc aaagtgtggg ccgaccttgt cgagagcttt gcgcagagga
     5701 cgcatcagcc cctcttctcg atcaggtcga gagtctctct gaggtacgtg ccgtactcgt
     5761 acttgctgca gcagacgaat gaacagagcg cgaggtcttc ttcggatagc tctagacaac
     5821 ccagcgcccg cgcggtctcc gtgtcgtgca cgatcagtga cttcagcagg ggagcgggca
     5881 gaatgtcgag cggcatgacc ttctcgaagt tgccgatggg gaccatcgca cgcgggcttc
     5941 cattctggga ggtggtgagc ggaaaccgat ggcttcgcgg tctcagcgca gacgtgaacg
     6001 cgcggatcga ggagaacctg ccaaagcccg gagacagcca attgaggaaa cgccggctcc
     6061 tgtcttcgtg aagcacgctg atctgaaggt cgtggggacc gaggtaccga gcgcgccccg
     6121 cagcgcggcg ccctcctagg atcgatcccg agatcacacg gcagtcggtc gcttcgagct
     6181 ccccattcaa gaggtcctga gtgctcgctc ccaagcgagt cctcagcaat cgcggccgac
     6241 ggacgacagg accgccgagc gcgatcacac gctcggtcga aagcctgccc gtcgtgaaga
     6301 gcgaaccgat ggcgatgacg tcctggtaac ccaagtgcca gacggacttc ttgtcgctga
     6361 caggatcgag gaagtgaatg tgtgttccag gcaggcctgc ggggtgcggt ccgtcgaaga
     6421 acgccgcggt caccgactcc aaatcgggaa gctcggcctg ggcgcctggt gactggcaaa
     6481 cgaacacctt gccgtcggta agccggctga gaacggcgat gccatcggag aaatcctgcg
     6541 cgcgttcggc aatgatgatg ctcgggtccg cagagagcgg attcgtgtcc atcgcggtga
     6601 cgaagatcga atgcggcgcg gcatcggccg caggaatgtt gccgaatgga cgtgtccgaa
     6661 gcgtggtcca catcccggag ctcaaaagga gatccagcac gtcggcgcga tcgagcttgg
     6721 agaggctgcc gcgttcgaac gcttggaatc gctgctcttc atcgccctcc aagcgaatca
     6781 cgatcgattg cagggacctc cgcggcccgc gatggatggc aaccacctct ccagccccag
     6841 gcgatgcgaa cacgacggaa gggtccgact tctcggtgag cagaggctgc ccgaggcgaa
     6901 catgatcgcc aatgtgcacg agtatgcggg gtcgtagtcc ctcgtagtcc tttgcaacca
     6961 acgccaccca gccgacgtca ttgccacttt cgatacgttg aacgggcgcg ccggcgatgg
     7021 ggatgttgag gcctttccga attcgaatgc gcatctgacg cggcggagtc taccccaaaa
     7081 ccttcggccg cgtgacagac tcactcgaag ccggccaaaa ttgccccacg aagacgcgtt
     7141 cgcacatgcg tcctgacgcc gctaaagctg gaccgcgacg agctagcacg gcgactcctc
     7201 atagagatgc ccgaggacgt cttccctcga gaggattccg atgattcgct ggccgtcgag
     7261 gaccggcacg cggccgatgt tgtgcgtcat catctgctcg agagcttcgg tgagcagctc
     7321 atcaggtcca atgctgtaga cgttttgcgc catgcagctc ttcacagaga gcgacccacg
     7381 accctcgcgc tcagcacgct ccacgtccct gcgagagatc acgcccacga gcttgccgtc
     7441 gtccacgacg ggagcgcccg tatgacccca ttcgtgaagc gattgagcca ggtcgcggag
     7501 aggcatatcg ggcgtgaccg ttcggaccgg gctcgacatg aggtcgcgca cgcaacgcgg
     7561 gctgcaactg agccgttcca cctgcgcctc caatctcatc accacttcgg ctagcgatgc
     7621 atgatgcacc acgccagacc ccgcacccgc gtgcccgcct ccgccgagct cctggaggat
     7681 cgcgccgact ggaacggtat tcgcttctgc gcgaccgatg atttgcgtct taccgctcgg
     7741 catgcggtac aggcagaaaa gggcatcatg aggcaggatc cggaacgcct cggtggatac
     7801 gcgggcaagc ccagcgggca cctcggtcgt ctctagggct gcgatcccga tcgaagctcc
     7861 aggtctcgga actgcgcgca cgccttcgag caagcgaacc aatagctggc gctccacggc
     7921 gtccagcggc ggacgcagga agcgttgaag aaggccgggc tgggcgcctt gccccagcag
     7981 ccaggccagc gccatcgcat cccgagagct cgaacgcgca taggccaacg agcctgtgtc
     8041 ctcgtgaatt ccgagcgaga acagcgttgc ctcctcgcgg tcgatctcga tgccgcgggc
     8101 ctgaagctcc tcgaccagga gagtcgtgac ggagcctacg ggctgcacgt gttcttcgtc
     8161 ggcatgcaag tcgtcggggg cgctcggatg gtggtcccag acgagaaccc ggacgcttcg
     8221 cgggttacgc gcggctcgct cgagaaccga ctcgacgtgg gtcaatcggc tccgccgacg
     8281 cacatcggtc accatcagca gatcgacttc cgcgaggtcg agatcctgag atcgctccgt
     8341 gcgaaaccga tcgccgtgga gggtcaaata ttgatgaacg ttgggggcga aaccaccact
     8401 gagccctatg accgagccgg ggaacagcct ctgcagagcc acgcacgatg ccagcgcgtc
     8461 gaagtcggtg gtctcatgcg aggtgatcag cttcacgtac ccgggtccaa catcccctgg
     8521 agaacgcgaa gcagatcgac gtaactcacg atgccaagga gatcgcccgt atcgaggtcg
     8581 gctactggaa tcgcgccgac gcgatgcatg agcatgagct ctaggatctc cgtcacgttg
     8641 gtctccggat cgactctgac ggggtcggtg ttcatgaacg tgccgatcgt cgcggatcgc
     8701 gcattctcga ccgcgtcgat cgcgtcgtcc tcgctcccct gtatgaaagc acgaaggtcg
     8761 cgatcgctca cgatgccagc caactcgcgt ccacgaacca cagggagatg ccgaacgtcg
     8821 agctcgtaca ggatcccgag tgcttcggcg acggacatga gctcggtcgc agtcacgggg
     8881 ttttcggtca tgatgtcttt tgcgagcaca agcttctcct ctcgacgtat tccgccaaaa
     8941 agctagaaca tggggaacgg caaggtcggt tcaaccgact cgaaatcctg aatgatcttt
     9001 agggccgtga ccactgctat cagcgagcag agatttgcgg cgaccaggct ccagcccatc
     9061 aaacgcaatc gtcgcgcggt gggctgaaga acggccatca cgagcaccac gactgccacg
     9121 ggtaccgccg cagcgagcac ccagagtccg gcgcgaacac catagggcgc cgcgacgccg
     9181 atcgcgatca cgagcaccga cacaaggacc agcaacacgc gcttcatgcg cacgcgcgcc
     9241 tgatcgctct cggtcttggc cctcgtccgc gcaacggtca gcctcaccac ggcggtcgcg
     9301 aggagggagg tgacgagcca gatgagcgcg accgccaatg tctccgtgag gcccaaaccc
     9361 gcagaaagac cgaggggaat ggcaatggag gacagcgtca tcgctgccag ggtttcgccg
     9421 accatgctgc gctcgagacc ctgaatggcc aacatcacca gtgaagctcc gagcacgagc
     9481 ggtaccccta ccgcgatctg agacgccctc ggcgcgagga ggagcccggc aatcccggaa
     9541 ccgagcgcga ctagaacgaa gatcaggagc gcacgctggg cgcgcccgct ctcctcgcgt
     9601 ttggtcctag tcccgcgctg cccaaccaga atcagcaagg gctcgttggc gaggaagcag
     9661 gcgatggcgc cggtcgccaa gagccaggtc gaggtgctcg gggcgccgcc cagaaagacg
     9721 gtgacgatcg ggaagaggag ctccgcgtag gcgccgtgtt cgcgcgggac catcttgcgt
     9781 tgtacggtgc ccatggtttc tgtggaatag caccgcttga ccctcaatcc atcgcatttc
     9841 gtgcgggctg gcggaggcgt cgcgcaatat ctgcgtattc gggtataagt agacccgcga
     9901 tgacctggca cttcgactgc gacgtgaccg acccactgat cgaccctagg acacggccat
     9961 gtggacggct gtagttgcag gggcggccgc gggccttgca tcggttccgc attgcacggc
    10021 gatgtgcggg ccgctggccg cttacgcctg tagcgggcgg ccgggcgtgt ccggacaggg
    10081 ccgctatcag ctcgggcggt ttttgagtta ctcggttctc ggtgccatcg ccgggacgat
    10141 cggcggcgcc accgccatga gccttcctcg agcctggggc ggcgccctgc tgagttggtc
    10201 tctcgctttg gggctcggac ttgcggcgct tcgtctctgg cgtcgtccga atcagcctct
    10261 agtgacactt cgaacgaagg acgaggggcc gcagccgtcg gtggtgtctc gcgcgctcgg
    10321 agggttgggc cgtcatccgt tcttcgtggg tctcgggacg gcgctgctcc cctgtggcgc
    10381 gctggctgca gcagtcctga tcgcggcctc atcgggctcg gccctactcg gctccctgag
    10441 catgctggcc ttttccgtcg tcagcggggt cgggctagtg ggcgcgacgt ttctcttcga
    10501 acgatttgca cgccgtccgc gacctgtgac ttcacgtgtt ttggcggtcg cactcgcgct
    10561 cggctcgatc ctattcgtca tccggccggt tcatgcattg cgtaccggcg ataccgcggc
    10621 ctgccatgca cccgccggcg agcaggccgc acccgatgaa cacaagcacc acgctcattc
    10681 gcagtgacct ccacgcagcc agccgtttgc gaagctcccc agcggcagtc cctgccctca
    10741 gtggcgcgct gcgttcattg ccagcagccc ttggtcaacg atagcctcgc accctactgc
    10801 tgccgtggct gccgggcggt gcacggaatt ctcacgaggg ccggactcgg ccgctactac
    10861 gagctgcgcc gcggcccggg cctgccgcca gccgacatgg aatcccgccg gatcgatcac
    10921 aaatggctcg agctcatcga agcggatcgg tccggcgaag ccggagttaa acgggtcgag
    10981 ctcgacatcc aaggcatcca ctgcgcggcc tgtgtctggt tgatcgaaga gctctttcgg
    11041 cgtcacggcg gcacacgaac cggcacgatg tcgatcaacc cggcacttgg gcgcgtggag
    11101 cttcggatct cagcacggtt cccactctcg gattgggtcg actcggtcga agacctcgga
    11161 tacctgctcg gtcccgcccg gtccacgggt gcaagagcat cggacggcct gctgctgcgc
    11221 gtcgggatct gcgcagcctt ggcgggcaat gtcatgctct ttgctgccgc gatctacctg
    11281 gggctccgcg aaggccccat ctaccagctc ctcaatgagc tcagctacgc ggccggtgca
    11341 ctcgcggtcc tggtcggcgg ctcggtattc atcggctcgg cggctcgggc catccgccgg
    11401 cgtgttcttc atctggatgt accgatcgct ctcgggatcg tcctcgcgtt tgccggctcg
    11461 acctactcgt tcttctttgc ctccgggcat gctgcgtaca tcgacacgct cacggtgttc
    11521 gtcgcgctca tgctcgtcgg gcgctggctt cagcaacgga tgctcgaacg caatcgtgcc
    11581 caattgctcg ccgacgacgg taccgaaagc ctgctgacgc gcgagatcaa agacggcgtc
    11641 gtgcgcatcg tccattgcat cggcgttcag gaaggcgacc ggctcctcat cgcccccggc
    11701 gacctggttc cggtggagct ccgcctgctc gacaagaccg caacctgctc gctcgattgg
    11761 atagacggcg agagccgtcc acggcgattc ggaagcggag aaaccgttcc cgcgggcgcc
    11821 ttcaatgtcg gggagcgtgc attcacggcc atcgcgacga gcgacttcgc ggcctcctcg
    11881 cttgtcggcc tccttgggcg ggacccggcg tcgagaggcg acggtcccct ggccacgcgc
    11941 tggtggtctc agctctcccg gtactacgtc atcggcgtca tcaccctcgc ggtctgcggg
    12001 atcggctact ggtggtggcg tacatcaaac ccgctcttcg ccatcgaggt cggcacggcg
    12061 gtactggtgg tgacctgccc ttgcgcgttc ggcatcgccg cgccgcttgc ctacgagctc
    12121 gtactcactc agttgcgccg cgcaggcgtc ttcgtgcgcg tcggcgattt cctcgaccgt
    12181 gtgcgtacga tgacacgcat cgtcttcgac aagacaggca ctctaaccac gggcatgttg
    12241 gaggtggtga atccgagggc gctcgaaacg ctctccgacc ccgagattac gctcctctac
    12301 agcatgtccg cacgcagtgc tcaccccaag agcgtagcgg tccgaagggc gctcgaaggg
    12361 catcgggccg cgcgcttcat cgagctcgag gtcgaagagc tgaccggcta cggcgtgagc
    12421 gccttcgaga atgggcgact ctacaagctc ggtgcgcccc actgggccgc ctctcgctcc
    12481 gtggatgatg gcgacgtcgt gttctcagtc gacgccgagg tgatcgcggt gctggaaacc
    12541 gacgagcagc tgcgcccagg cgcccggacg gaaatcgagc gcctcgagag cgagggttac
    12601 cagctcgcga ttctgagcgg ggacacgccc tcacgggtgg tgagcctcgg ggtgagcctg
    12661 ggcatgccag aggagctctg catcggtggt cagaaccccg aacagaaggc cgcgtacgta
    12721 cgcgagcacg aaccggcacg aaccttgctc gtgggggacg gcttgaatga ccaggtcgcc
    12781 atgcgagagg cggcttgctc gggcacccct gcggtgaatc gaccgttcat ggcatcacga
    12841 tgtgatttct acttcgtgtc gccgggttta cgccctgtgg cgcaagttct gcacgcttcc
    12901 gacgtgctcg cgcatgttgt gcgccgcatc ctcgcgtttg caatcgctta caatctggtc
    12961 gccgtttcgc tcgccatggc cggattgatg aaaccttggg tcgcagctgt gctaatgccg
    13021 ctcagctcgc tcgtcacgct tgcttacacc gtgatgatgc tgacgcgaaa agggctctca
    13081 tggagatctt gatcttactg attttcgttt gcgtagtgct ggttggcgtc gccgtggcgt
    13141 tcttcgcgtg gaccgtgcgg cagggaacct tcgatcatgc cgatcgcctt gccttgctac
    13201 cgcttgacga cgaaaagccg atgaccccaa cgacggagag ggaagaggac gagtatggaa
    13261 actcagcgga tcgtttataa cgacaaggtc gttcgccagt ttgtgtgggc gtcggtgctg
    13321 tttggcatcg tcggcatgct ggtcgggatc atcatcgccc tgcagttggc gttctggccg
    13381 gccaaccttc cgccgtacct atcgttcggc cgcctgaggc cattacacac gaatgccgtc
    13441 atcttcgcat tcgtcggcaa catgcttttc gcgggtatct attactcgac gcagcgcttg
    13501 gccaaggcgc gcctcgcatc ggacatcttg tcttcgattc atttctgggg ttggcagctg
    13561 atcatcgtct ccgctgcaat cacgctgcca ctcgggatca cgaccgccaa ggagtacgcc
    13621 gagctcgaat ggccgatcga catcgcgatc gctctgattt gggtcgtctt cgcggtgaac
    13681 ttcttctgga cgctggccaa gcgccgagag aagcatctct acgtcgcgat ttggttctac
    13741 atcgcgacca tcatcaccgt cgcggtgctg catatcgtca atagcttcga gatccccgtc
    13801 gggctcttca agagctactc cgtcttctcg ggcgttcaag acgcgctcgt gcagtggtgg
    13861 tacggccaca atgccgtcgc cttcttcctc accacgccga ttctcggcat catgtattac
    13921 ttcctgccca aggctgccga gcgaccggtg ttctcatacc gtttgtcgat cgtgcacttc
    13981 tggggcctgg tcttcctcta catctgggcc ggaccgcacc atctgctcta caccgcgctc
    14041 cccgactggg cacaaagcct cggcatggtc ttcagcctga tgctctgggc accgagctgg
    14101 ggcggtatgt tgaacggcct tctcacgctt cgtggcgcat gggataagct tcgggccgac
    14161 cccgtcctga agttcttcgc tgcaggcgtg accttctacg ggatgagcac attcgaggga
    14221 cctctgctca gcatcaagag cgttagcggt ctcgctcatt acaccgactg gatcatcggc
    14281 cacgtgcaca gcggtggcct cggctggaac ggcctcatgg cggccggcat gttctactgg
    14341 ctggtcccga ggctctacgg cacgaagctt cactcgcgcg cagccgcgaa cttccacttc
    14401 tggatcgcca ccgtgggcat tcttctctac gtggtttcga tgtgggtcgc cggcatcacg
    14461 cagggcctca tgtggcgcgc ggtcagcgag accggcgggc ttctctaccc gaacttcgtc
    14521 gagaccctcc tcgccatcaa gccgatgtac tgggcgcgcc tgactggcgg tctgctgtac
    14581 atcactggat tcattctgat ggcctggaac ttgatcaaga ccgcgaagag cggggctcca
    14641 gtggacggcg agatcgaggt tgccgtggtg accgatccgc gcgaccgcga gatcccatgg
    14701 cccaagctcc tcttcggtca gccggtcatg gcagcgatca tcgtgatggg gcttctgttc
    14761 gcgatggggc tcttcgacgg cttcatcagc acggtgcttg ccctcgtcgc cctcatgtgg
    14821 gctgtggccg cgatcgccat ttcgatccgt ggtcgcgacg aggataagat ctcgtggcac
    14881 cacgcgctcg aaggccgtgc cggtgtcttc acggtgctcg tggtcatcgg catcttggtc
    14941 gggggagttg ccgagatcat cccactggtc atctcggtgc cagagtccat ccgcaccacg
    15001 aagaacgtcc cctacacgcc gctcgagctc gaaggtcgtg acgtcttcat tcgcgaaggt
    15061 tgctatacct gccattcgca gatgattcga cccttcacct gggaaaccgc gcgctacggg
    15121 gcggtctcga cgctcgatga ttcgctcttc gatcacccgt tccagtgggg ctctcgccgg
    15181 ataggcccgg atctcgcacg ggtcggccgc aaatacgccg acgtctggca ctacaagcac
    15241 atgatcaacc cgcgagagat ctcgccgggt tcgaacatgc cgccctaccc acacctggcg
    15301 accgagacga tcgacttcgc caacacaacc gtgaagctga gggccatgcg aaatgtcggc
    15361 gttccataca aagccgatca gattcaaacg gccgaagccc gcgcccaggc tgaagcggca
    15421 tcgatcgcgg caggcctgtc cgagagcgca ggcgtgaagg tctgtgacac gccgggcgaa
    15481 ggctgcgagc tgctggtcaa cagccgcctg gtggccctga tcgcctactt gcagcgcttg
    15541 ggtcacgtgc ccgagggcga gccactcgcc gtctccaaca ccacggaggt ggcgccatga
    15601 gccgttttgc gtcggagttt ttcgaacaga gccctgtgct tctctatccg gtgctggcgc
    15661 tcgttctatt tttcatcgtg tttctagtcg tgatcgttcg cgtcatgcgg atgaaggcat
    15721 cggaggcgga tctctgcgcg cgcattccgc tcgaagagga gttggaggta ccccatgagt
    15781 gaggccaagc gaacagacga aatccaaggc gagatcctcc acgtctatga cggcatcgag
    15841 gaagccgaca acaacctgcc gacctggtgg cttctcacgt tctacggcgc ggtcgtcttt
    15901 ggattgatct attggttcta ctatcacgag tacgccatcg gccagctgca gccccagaag
    15961 tatgcggagg ctgtcgcagc cgcggaagcg gagcggagca tcgtaaccga cgagtccctc
    16021 gacgcgttgg cgctcgatga atccgcagtg actgccggaa gtcagatatt cgccacacaa
    16081 tgcatggcct gccacgacag caaggcgcag ggacgagtcg gtctgggacc gaacttgacc
    16141 gacaagtact ggattcacgg aggcgctccg accagcattc acgctaccat caccaatggt
    16201 atcgcggcca agggcatgcc tgcctgggcg ccgattctgg gagaagaagg cgtgaggcag
    16261 gttgtcgcat acgtgctcac ccttcggaac acggaggtgc ctggcgtcga gccgcaaggt
    16321 gaggtttggg aacctggtgg aaccctggca gacgacgccg ctggctctaa gagtccatga
    16381 cgcatgacaa agctgccgat cgtagaacaa cccgctagct ccctgcgctc ggacggaagc
    16441 cgaaactacg tacacccggc agacgtgtcg ggccgcttca cccgtctccg atacaccatc
    16501 ttcagcgtcc tcatcgcgat ctacgtcgta ctgcctttcg tgaaaatcgg tgggcacccg
    16561 gcagtattca tcgacatcgt caaccggcgc ttttatctgt tcggcgccgt attcaatgcc
    16621 caggacttct ggctcgtctt ctttctcctc agcggcgtcg gcctcgcgct tttgatcgtc
    16681 accacgatca aggggcgcct ttggtgcggc tacgcctgtc cgcacaccgt cttcttggaa
    16741 ggggtgttcc gtcgcatcga gcgcctgatc gaaggaccga gagcccaacg gctacgtcga
    16801 aacgcggagg ggctcacctt cgacaaggtc tggcgcaagg gactcaagca tgcggtgtac
    16861 atcgtgcttg cgctgcttct gtcgcacgtc ttcatcagct acttcgtctc gctcccgtcc
    16921 gtgctcgaga tggtgcagcg gagccccgcg gagcacccga ccgcctttac ctgggtcgtc
    16981 gtgatgtccg ggattctgta cttcaacttc agttggttcc gcgagcagct ctgcttgatc
    17041 atctgtccgt acggacgact gcaatccgcg atgaccgatc gtgacaccat ggtcatcggg
    17101 tacgactgga accggggcga accccggggc aaggcgagca acccggagaa cggcgactgc
    17161 gtcgactgca aacgctgcgt agtcgtctgt cccaccggga tcgacatccg caacggccta
    17221 caaatggagt gcgtcggctg cgcggcgtgc gtcgatgcct gtgacgagat catggagaag
    17281 gtcagcaggc ctcaaggact ggttcgctac gattcgctga acggcctcga gggcaaagcc
    17341 aagcacctcg gacggccgcg tttgttcttc tacggcggcg tcgtggcgct atggttgatc
    17401 ggtgccgtgt tcgccgttgc tcagagcgca gacttcgaaa cgaatgttct tcggctcacc
    17461 ggtgcaccct tctcagtcgt cgacggccag gtgcgcaatt cgttgcgagt gcatctcgtc
    17521 aacaagaccg gccagaagca agcgtttctc gtcgtaccag agaccgaaag cggcatcgaa
    17581 tacatcgtgc cgcagaagcg ggtcgagctc gagtccctcg gcagcaccta tctacccatc
    17641 ctcgcgatcc tcccgcgcga gcagatgcgc cccgggctga aggtccaact cacgatctcg
    17701 atggagggcc gtgacgaccg aaaggcgagc cgccttgtgg aggcgccgtt cgtcgggcca
    17761 ggatcctagc gcagcgcctt actagggtac cgacatcacg attgctccgc tcttgtggac
    17821 gagctcgtgt gagacgctac cgagcagcgt gcgcgccacc gcccccttac cggtcgtgcc
    17881 gcagataatg aggggagcat cgatctctct ggcgaccttc acgatggcgt cggcgggatg
    17941 gccggtcacc gcgtgacatc gaatctggag gtcttcggac tgcacggtgg ctgaggtccg
    18001 caattcgagg agctggcgca agtcctcgat ttcctttccc gagctcgatg cctctgactc
    18061 tttagagtcg agcaccgtaa cgacgtggac ttcagccttg agctgccgac ccatgaatac
    18121 cgtaagctcg aacgctcgcc tggcagggtc ggaaaaatcg taggcgacaa ccaaacgggg
    18181 aatcagtgac acgggctact ctccatcttt gggcttgttg tcacaacggc cacgcacgac
    18241 caagacagga caaggcgcgt ggcgaacgac tctctccgct acgctgccga ggaacgccct
    18301 cgacagccca cttcggccgt gggagccgag aatcaacaga tcaaccgctt cgtcctcggc
    18361 catctccacc aaggcccgac ccggggacgg gtgctggatg acgctcagct ctacctcggg
    18421 aacccctttg aggagctcgg cggtcttacc gttgagcgtc tcgcggatcg cctcttcgat
    18481 ttcgacgcga atgctgcgct cgagcaggtc tgggcgcgac cagacgaccg gaggcacgtt
    18541 gggcgtcgga tcgaacacat gtgtcaccaa gacgcgcgag ccgaaagtct tggcgtagag
    18601 agcggcggcc cgaagcgctt cgtccgagat gtcggagaag tccgtgccga cgaggatggt
    18661 ttgcggtttg tccatgagta cagccttgcg tatcaagctt cgtgccagac gaaagccccc
    18721 tgtttggcgg ggccagcata gcgaaaatgg cgcatcttct gcgaaaattt ccatccggac
    18781 gcagaacttg cgtgccgcac gaagcgaaat gctgctaaat cattggcgcg gtctttgcag
    18841 actctccaac cagcatgctc caagggagga caagatgagt gacgagccgt tgatgccttc
    18901 cgaggtccga cgaagggtcc tcagccagca ccgggagatc gagcaaatgc tggctgagct
    18961 cgaaactggt gtcaccaagc tgaatgacgg aagcgtggac gccggccagg tcaagcgtgc
    19021 ggcgtacgct ttgcgcggaa tcctggagct tcacatgagc ttcgaggaaa cccacatggc
    19081 tcccgccatc aacgatgccg atggcttcgg tcccgagcgc gtgcggcatt tgcatgccga
    19141 gcatgccgac caacgcgcgg agatggaccg gttgataggc tcgattcggg aagccgagtc
    19201 cagggctgag ctgaccaaga gcgtcgtcaa gctcgccgag atgctgcgca tcgatatcga
    19261 agaagaggaa cgcgagtacg tgaacgagaa gctcctcagc gaccacctga tcccaagcga
    19321 cccatttggc ggctagcggc cccaatcgag gcgacgaaag acatcatatg cagatcaagc
    19381 aaatcctagt tccgacggac ttctcggaga acgcgcaaca cgccgtggac tacgcgaccg
    19441 agctggccaa gaaatgctcc gcgaagctgc atctcctgca cacgccggtc gttccgacct
    19501 acctattgat ggacctttcg tacagtccag gacctgaggc ggtaacgcgc atcttgaacg
    19561 acgcgcagga ggccctcgac cggcaagcca agagccttgc aggctccggc gtggagctct
    19621 tcaccgcgat tcgagaagga acgattcacg aggtgattcg agactacgcc aaggagcacg
    19681 acgtcgattt ggtcgtcatt ggcacgcacg gaaggacggg cgtctcgaag ttgatgtatg
    19741 gcagcgtgac cgagcgagtg atcaagacgg tccacacgcc gatcatcgtg atcccgccag
    19801 tgggcggaaa attgccgagc tcgatcgtca tcgcatacga cttttctgga ccctcaaaac
    19861 gcgccgccga gattgctcac gcaatccacg acgtttttca tgggccgctg cacatggtgc
    19921 actgctatct cgacgtttgg ggcgaataca ccgaccgagg cgccgtagtc ggcgaggcag
    19981 cagaaaagcg ccgcgaggca cttcatcttg ggctcgagga aatgcttcaa accgaagcca
    20041 aggagctctt ctccgaagac ccccaaggcg ttcaaaccca cctactgacc ggtgatcccg
    20101 cagagggctt gctgagcgtc gcaggcaaaa cgggcgccac cctgatctgt gcgggaacga
    20161 cgggaaagag tggcatcgag cgacttctca ttggtagcgt cgccaggcgc ctcctccacg
    20221 acagtaaggt gcccttgctt ctcactcatg acgacggctg aagacacagc gtcttgaaag
    20281 agcgctcgaa ccgcgcatga tagggccgtg atgctcgggc gggtcgtcgt aatgggggta
    20341 gtggctgcac ttttgagtgg ttgcgagtgc gagccgaagc gcttgcctca ggcttcggag
    20401 acccctgcgg gcttcgacgt ggtcatgaag gagcacggcc agctctcgat catctcgagg
    20461 aacgcgctca ttcgaggtga gctcccgctg gcacagcagt cgatgcggaa gctgctgttc
    20521 ttcatggagc acgtgccttt tcccgagccg ggcaaggagt acgcacggat caccaaggag
    20581 ctcgccaccg aggtgaagga ggcgagcgac atggaagagg catgcatggc gttctcccgt
    20641 ctctcccatg cctgcggtca atgccaccac gcactcgacc gggggccccc gatcaagctc
    20701 gagcctgcag cgggaggtga cgacctccgc atgcacatgc ggcggcatta ttgggccatc
    20761 gaacgcatgt gggaggcgct tctgagtgat tcgcctacgg cgtttcaggt ggccgccgga
    20821 gtcctggccg aatccccgct tcacggaagc caccagccgg acggagacaa cccgcccggt
    20881 accacccgcc tcgagtacga ggttcatgac cttgctttcg ctgccgccgt cgaggggcgg
    20941 attagcgaag acgagtacgt gccgaaaccc ggcgagctgc tcgaggacgc gccgaccacg
    21001 ctcgggcagg ccgagatttt cggtaggctc ttgtttgcct gcgagcagtg ccatgcgctg
    21061 gtagacgtga aaccggcact gacgcccgcg acgaaccgag gagccgtgcc gtgatgctgc
    21121 aacatcgaaa gcagaccctt cgcgctgcca tggtgctact gctcctcgcc gcggcctgcg
    21181 ccagcggacg cgtcagcggc ggtcaaggca ccgaaatggc acaggaagaa gcatacgagc
    21241 ccagcaacgc tgcgaatcct ccgaccggaa acaccgactt cacttcgggg ccaatcaagg
    21301 ccatcacgcc ccctccggtc ctacaagtcg acctcgagcg acgccgaccg ccgaccgggc
    21361 cgcccatcga gcccgagccg gaaccgcaat ccaaccccga aacccagaac gagcccaggt
    21421 aagccaacga gatgtccaag ctttgcaccg ccgaagaagc ggttaatcac gtcaattcga
    21481 atcagcgcgt cttccttcac ggcggcgtgg caactcccac taggctcgtg gatgcgctgg
    21541 tccgagacgc caaccggctt cgcgacgtcg agctcatcca cctgcacacc tctggcgatg
    21601 gggcttacgc gagacctgaa tacgagcgtt cttttcgagt cgcgaacctg ttcgtcggcg
    21661 agaacatgcg acaccaactc gacggcgacc gcgtcgacta tctgccctgc ttcttgtccg
    21721 agattccaca attgttccgc tctggccgac gcgcgctcga tatcgcactc gttcaggtct
    21781 caccgcccga taagcgtggc tactgcagcc tcggcgtatc cgtcgacgtc gccagagctg
    21841 ccgtcgactc ggcgaggatc gtgatcgcgc aggtcaatcg tcacatgcca agggtccatg
    21901 gggacggttt cgttcacatc gaccaactcg actggctcgt ggaacacgat gagcccttgc
    21961 ctgaagttcc ggcgcggcct ccaaccccag aggaaaaggc aattgggcac cacaccgccg
    22021 agctcgtcga agacggcgcc acgctgcaaa tgggtatcgg cgccgtgccc gacgcggtgt
    22081 tggcatcgct caagggccat cgccatctcg gggtgcatac cgagatgtgg tctgacggct
    22141 tgctaccatt gctggaatgc ggggccgtcg acaacagcca aaaggtcgtt catccgggtc
    22201 gtacggtttc tggtttcgtg gtgggaaccc gtcgtctcta tgactacatc gacgacaatc
    22261 cgaccgtcac acaatacgac atcggctacg tgaatcgagt caacgtcatc aatcgaaacc
    22321 cgaaggtgac cgcgatcaac tccgccgtgg agatcgattt gaccggccag gtctgcgcgg
    22381 attccatcgg acatcgggtg atctccggcg tgggcgggca aatggacttc atccgaggcg
    22441 cgatgacttc ccagggcggc aaaccaatca tcgccctcac gagccgcacc ccgaagggca
    22501 ttcctcgcat cgtgcccacc cttcggcccg gcgcgggcgt ggtgacgacg cgggcacacg
    22561 ttcagttcgt cattaccgag aacggcgcgg tcgatctcta cgggaagacg ctcaacgaac
    22621 gcgctcagtt gctgatctcc atcgcacacc cggacgacca agagggcttg gagcgcgctt
    22681 ggcacgacgc gcgggcttga gaagacgcag cgcttgcgcg ccccgcgacc gcggccgcag
    22741 aagttgcgcc gcggctttac gaatgtctgc gacttgtgac ggaaaggccc tgcgtggagg
    22801 gccaggcttt ggcataactc ctgcttgggt agagggggtt tcgacccagc ctcgttttgg
    22861 gtctaagggg agcttattgt gattctgccg agttggtttc cgtctcgctc tgacagtggg
    22921 tctgggcttc gtcttctcag cagggctcca gggagcgatg cagacgtgga cgctttgccg
    22981 gctgccgcga tcacgcccga cgcagagtgg ttgagtgtca acgccggcgg ctcgttcgcc
    23041 atgggcacca tcgaccggtg tcctcgccgc aagtaccacg ggctgctgac agtgcgggag
    23101 ccgggggtgg gagagccgct caacatggtc gccgaagtcg aggagtggtt ccggattggc
    23161 ggcgagcgca tcgcgctcca cagctacaac tggggcaccg ccatcgagcc ccacggaatc
    23221 gactacctga gccggttcga gccgcaacct cgttggacct atgagctcaa aggactcagc
    23281 gtcacccgtg agctctggct cgagaacggc gctgatgcgg tatggattcg ctactcgatc
    23341 gaaggtctga ccgaggcggt cgatttgagg ctacggcccc tgatccggtg ccgtccggtg
    23401 cacgagttga cgttcgcaaa ccccttcatg aacggggagc tggacatcga ggacggtaag
    23461 tcgcttcgcg tcgtgatgca cccgtacaag ggcgtgccgg cgttgatcgc cagcttgcac
    23521 ggggcgccgg gcgtgtttcg cgccgacggt cactggtatg aaggcgttca ctacgagtgg
    23581 gagcgcgagc ggggctatga cgacgcagag gatctgttca cccccggcga gttccgtttg
    23641 caactcgagc acgacggtgc gttctgcatc cggatcggaa cgacacctgc cgcaagccat
    23701 gagccccagg tgctcagctc ggtggtggat tcgcgtcggt cctttctgaa agagctcgac
    23761 gacgccgccg aagcctactt ggtggagctc gcgggcggcc ggacgggcat catcgcgggc
    23821 tacccctggt ttggcgagtg gtcccgggac gccctcatcg ctcttcccgg tctctgcctg
    23881 gcgcggggcc aggtcgacaa ggccttggcg attctcgagt ccttggccga tcgtcgcgaa
    23941 aacggactga ttccaaacat tccacaaagc ggcgacgtcc cgtcgaacgg ggagtcgatc
    24001 gacgcttcac tgctcttcat ccgggccgtg gcgttgaccg cgaaacgaac acgcagtcgg
    24061 cggattgcgg ccctccagga agtctcattc gacctgatcg agcaaatcgc cgcgggcgcc
    24121 gaccctcgag tgcatgtcga cccgcaaggg ttcctgttcg tggatcacgg accctgggcg
    24181 ctgacctgga tggatgctct cgtggacggg tggccggtga cgccccgtca gggctatgcg
    24241 gtcgacctca acgcgctatg gctctccggt ctcggggccg cgctccgctg ggcgaagaag
    24301 cttcggccgg agttcatcga aaagtggagc gaaaccgcga agaaactctc cgatcacttc
    24361 gaagaaaagt tctggcttca agatcagggc ttcttgactg acacgcacga tggcaaccat
    24421 ccggacgggc agctgcgacc caaccagctt tgggctctca gtctgccggg cgtcccgatc
    24481 gacaaggaac gaaagaagcg ggcccttcaa gccgtgcgcg agaagctcct cacgccagtc
    24541 ggcctgcgaa ccctctcgcc ggacgacccg gcgtaccggc ccttctaccg aggggggcag
    24601 acagagcgcg accgggccta tcatcaggga acggtttggc cctggcttct cgggatctac
    24661 gcagatgcgg tcgaggcaat cgaaggatcc gatgcggcgg tcgaagaaat gatgccggtg
    24721 ctcgctgcac tttctgcaca tcttcgtacc gagggttgca ttggacagat cgccgaggtt
    24781 ttcgacggcg acgcgcctca ccgaccagga ggcaccccag cacaggcctg gagcgtgtcc
    24841 gaggttctgc gtgtcgcaaa gatggccggg ccatgaagat cttgatgctg ggatgggagt
    24901 atccgccgca catcagcggc ggactcggaa cggcgtgtga agggctcacc accgcagtcg
    24961 cgagccgggg catcgaaatc gacttcctcg tgccgaggct gtacggcgag gaggacgcgc
    25021 cgcacatgcg actacttggc gcggcttccg cggaccccat ggtccaggac gagagtccgc
    25081 ccccagtcgc gaccatgagc cacttggcag agctcatcgc cgcagagcct gcactcgcca
    25141 ccgacccgat cttcgtggcg gctgtggagg ccgcgagcaa gggaatgcct caggactcgg
    25201 caggcgagat tcgcacgatc cgcgtgccgg cgatgctcaa gccgtatctc gaccaggcaa
    25261 cataccggga gctcatcacg agtggcgcga tttcggagat cgcgctctct caaatcgccg
    25321 caacgcaaat cgcgcacccg gtccccgatc ctccaggggg cgtcactcgc cttcagcacg
    25381 ctcacgtccc gggcggagac ctgcctctcg cggagcagcc agcaacggga gcacactacg
    25441 gacacgatct cttcgcggag gtcgcgcgct atgcaatggc tgcgacgcgc cgcgtcgccg
    25501 gtctcaagta cgacctggta cacgcacacg actggatgac ctttcccgca ggtatcgaga
    25561 tcgcgcgccg agctggcgtc ccgctcctcg tccatgtgca tagcctcgag tacgaccgcg
    25621 ccggtcgtgg agccgaccga gacatcgttt ctgtcgaacg ccaagggctc gagcaagcta
    25681 cgcgagtcat tgcggtgagc tactacaccc ggagcctgat caatcgtgtg catggaacgc
    25741 cgctcgacaa gatttcggtc gtacacaatg gcgtatacga acgcgaaacc gtcgaggctt
    25801 acaccgagca acgatcctcg aaagggccgg tcgtgctgtt cctcggtagg gtcacgttcc
    25861 agaaggggcc ggagtacttc gtccaggccg cggcgcgtgt tctcgagcac gttcccaatg
    25921 cgatcttcgt gatggctggc tcaggcgaca tgcttccgcg catgaaagcc ctggtggaag
    25981 agctgggcat caccaaatcg ttccggttcc cgggtttcgt ccgtggtgcc gagctcgagc
    26041 gaacgttctc gacggctgac gtctacgtca tgccgtcggt ctcggagcca ttcggaatcg
    26101 ctccgctcga agccatgagc tacgaccgtc cagtcatcgt ttcgaagcaa tcgggcgttt
    26161 cggaagtcct tcaaaacgcc ctcaaggtcg acttctggga cgtcgacaaa ctcgcgagcc
    26221 agatcgtcgc gctcctcgag cttccagagc ttcgccgagt catcgtcgaa cgcgcgcgag
    26281 aagagatccg gcacatccac tgggatgctg ccgcggaaaa gctcgtaccg atctacgagt
    26341 cggtcgcacg tggttgaggt gaatcatgcc gtcggtttgt ttctatttcg aggtgcatca
    26401 gccctaccga ctgaagccat atggagccct tcaagtggga cgcgaccatg agtacttcga
    26461 cgacaagctg aacgccgagg tactgcgcaa ggtagccagg aagtgctacc tcccggccaa
    26521 cgaatcgatg ctgcgcttga tcgaccgcac cgacggtcgc tttcgcgttt cctatgccgt
    26581 gactggcgtg gcgatcgaac aaatgaagct tttcgcgccc gaggtcatgg acagcttcgt
    26641 caggctggcc gagaccggcg cagtcgagtt cgtcgccgag acgtactatc acagcctgtc
    26701 ggccctctac gacaaggatg agtaccgcga gcaaatcgag ctcgagatga agctcatcaa
    26761 gtccgagttt ggtcaaaccc cgcgcgtgtt tcggaacacg gagctcatct acgacgacgg
    26821 gatcggccag ttcatctcgg atctgggctt cgacgcaata ctcgtcgaag gtgcagacga
    26881 cgttctcgat tggagaagcc caaacttcgt ttaccgggtc actgatcgaa agacgcgcct
    26941 gctcccgaag aactacaagc tctcggacga catcgcgttc cggttttcca accggggttg
    27001 gcgcgagttt ccgcttactg cagagaagtt cgctggatgg attcacgggc acaccggggc
    27061 gggcgacgtg atcaacctgt tcatggacta tgaaacgttc ggtgagcacc agtggaagga
    27121 gacaggcatt ttcgagttcc tcgagctact tccggagctc atcttcacgc atccgcattg
    27181 ggacatcctc acgccaagtc aggccatcga ccgatatccg gccatcggag agctaccttt
    27241 tcaccgaacg gtttcctggg cggacgaggc gcgggacatc agcgcttggc agggcaacgc
    27301 catgcagcag cgcgccctca gggagatcta cgccctggga ccggctatcc ggcgacgtaa
    27361 taattctcac ttgatggcgc tttggcgccg cctgcagacc tcagatcatt tctattacat
    27421 ggccacgaaa tccgctacag atgcggcagt acatgactat tttagtccat atgacgggcc
    27481 atacgacgca ttcatccact atatgaatgt tatgaaagac ttgaagctct cggtccttgc
    27541 gcccgcagag aaacagattc gactatgagc aaagcagtat ttttcgagat ctcctgggaa
    27601 gtctgcaaca tggtcggtgg tatccacacc gtcttggcga gccgggtgcc cgaagtgcag
    27661 caacgccacg gtgaggacag gtatgtcacg atcggtcctg acgttcctcg aaccgagggc
    27721 gtcgcccctg agttccgtcc cgacatctgg gacccagagc tggcagagtc actgaaggac
    27781 cacgaggtca gtgtcctcat ggggcgctgg ttggttcctg gcgagccacg ctgcttgctg
    27841 gtcaatcagt cacgcttgta cgagcgcaag gatgagatcc tcgggcgata ctgggaggac
    27901 tacggcctcg attcattgtt tggcgcctgg gactattacg acccggtttt gttcgctcat
    27961 ggcgcaggca tgatcatcga gcacatccgc gatcgctttc tgcttcccgc ccgtcaacgc
    28021 gcgatcgtcc aagcgcacga gtggatgtcg gcgggcgcga tcctccactt gaaaacggcg
    28081 gcgcccgaga tcgggaccgt cttcacgacc cacgccacga tgctcggtcg ttcgcttgcc
    28141 ggacggcgcg cagatcccaa cttctacaag tcattggccg acgtagaccc cgagcttgcc
    28201 gccaaagagc tcaacgtctc atccaagcac tcgatggaga cggtagcagc ccgcgaagcg
    28261 gatgtgttca caaccgtcag cgagatcacc gcactcgagt gcaagtatct gctggatcga
    28321 tcgccagatg tgattcttcc caatggtttt ggagcaaagc ccgttgcacc tgagctgcgt
    28381 caacgagccc gcgaggagct tttcaagctg gcggagctca cgacgggcga cacctacgac
    28441 agagacaaga cgctgatctt cgctcttgct ggacgctacg agtacatcaa caagggtgtc
    28501 gacgtttacc tcgatgcggc caccgcactc gcgcaagatc tcgagtcccg cgacggcaaa
    28561 cgcgtgatcg tctatgcaat gcttccggcc ggtcacgcgg agcccaaacg ccagctctgg
    28621 gatcgagcgc acggcgcctc ggctggagct ccgcttcgct gcacacatga cctcgttgac
    28681 gaggcgaacg atcccatcac gaaccgtctg aacgctcttg ggttgaacaa caacccagga
    28741 gcccgtgtgc acgtggttca tgtgccgatc tacctcgacg gaacggatcc tttgatccgc
    28801 cagaagtact gggatctgct tccgggtgcc gacctcggcg tgtttccgtc gttctacgag
    28861 ccctggggtt acactccgct cgaagcggtc gcctttggag tgccagcgat cacgacggac
    28921 cgcgcggggt tcggacgctg ggttgcggcc cagggcgatg ggaaccggac tggagtgagg
    28981 gtgctcaagc gggagggcgt gctcacctcg gaagtgacca aggcgttgca ggacgccatg
    29041 ctcgagttca tcgatctcgc tcaggcggat cgcgattcgc tcggcgaggc ttgcctgcgc
    29101 acggcggctt tgaccgactg gtcgcacttc atggagcact acgaggaagc gcatcgtctt
    29161 gcactagagg ctggggcagc ccgccgaaaa gggctaccga tggaccgact gagcgcgcca
    29221 tcgatgccag cgctttcgtc ggactcatcg ggggtcttcg ggctcttcgt aaagccgccc
    29281 gtcaaggagg gcgaagaggc cgctccctac acgcgttcgt tcatggtgac caacgccctt
    29341 cccccagagc tacagggtct gcagggcatc gcctcgaatc tctggtggcg ctggcacccg
    29401 gaagtcgcct cgctgttcaa gagaatcgac cccgcactct ggttcaagat cgaagaaaat
    29461 ccgcacgcgc tcctcgacca ggtgaagccc gagcggctca tggatttggc cttgaacgag
    29521 gggtacgtcg ccgatgtcgc acggctccat cgagagatgg tcgagtcgac tgagacgcaa
    29581 gaccctcgga tcgcctactt ctgtatggag tttgggcttg ccggcttctt gaagctctac
    29641 tcgggaggtc ttggtatcct cgccggcgac catctcaaag ccgcgagcga tttgaatctg
    29701 cccttgtgcg cggtcggcct cgcctaccag gacgggtatt tccgccaaat catcgatcgg
    29761 gatggccacc aggaggacct gggcgacacg aacgatttcg gaagcccacc ctatgagcaa
    29821 gtcatgactg ggcaatatgc gccgctcagg gtgaccgttc cgcttccaac cggcccggtg
    29881 caagtgcaag cctggcaact caaggtcggc cgggttccct tgttcttgct cgataccaac
    29941 gtgcccgaga accgtcccga ggatcgcgcg atcaccaacc gtttgtacgg cggcgacgct
    30001 gcccatcgtc ttcgacaaga gatgattctc gggcttggcg gtttccaagt gctgaccgag
    30061 ctcgacatcg acccggacgt cttccacatg aacgaagggc acagcgcatt cctcatcatc
    30121 gcgcgcgcgg cccacttgat tcaacgtcac ggcctccgct cccaggaggc cctcgagtac
    30181 attcgcaaca ccacgatctt cacgacgcac acgccggtgc ccgcaggaca cgaccacttc
    30241 ccagaagaaa tggtgcgccc ctacctgacg agattcgagc acgtgcttcg catggactgg
    30301 ccgcgtttga tggcgatggg cagcacgcca agcggcgcgc agaacaagtt tggaacgacg
    30361 gccctagctg tgagaaacgc aggtcgcatc aacggtgtga gcgccaagca tggcgaggtc
    30421 gcgcgcgaga tgttcctgca gttctatcca gagtacgaga acgccaacga cgtgccctgc
    30481 actagcatca ccaacggcgc gcacgtgtcc acttggctcg cccctcgctg gcaacggctc
    30541 atggaacggg aaatgggtca cgactggcgc agcgagattg aagatccgta tcgctgggaa
    30601 tggttgcgca atcatgacgc gtccgagatc tggtcgattc atcgggaact tcgggcggac
    30661 tacgtggatt ggctcaaagg ccatctcacc gagacctgga cgcgtcggcg cgaagacctc
    30721 tctctgctgc aagacatcct ctccaggctc gatgaggatc gcatgttcat cggctttgcc
    30781 cggcgcttcg ccccatacaa gcgtgcggac ttgctgctca ccgagccagc gcgcctcgcc
    30841 aaactgctca acggcaaccc gggtgcgaca atcatctatg cagggaaggc ccatcctgca
    30901 gacggccacg ggcaggcgct gctgcagcga gtctatcagg ccacgaagca tcccgacctc
    30961 gcgggacgcg tgatcttcat ggaaggctac tcgatcgact ctgcgcgtcg catggttgct
    31021 ggatgcgacg tctggctcaa tacgccaacg cggccgctcg aagcgagcgg cacttcgggg
    31081 atgaaggccg cgatgaacgg ctgtttgaac ctcagcgtgg acgacggatg gtggctggaa
    31141 gggtacgaag agaacaatgg atgggtcatc gacctacctg cgacttcgat ccaggacccg
    31201 agtgcctacg accgtgccgt cacgatggca ctgttggagg gagagattct gcccacctat
    31261 cacgatcgca acgagacagg cgtccccatt gcctgggtcg acatgatgaa agccagcatg
    31321 gcgagcatca tcccgaagtt ctccgccggc cgtatggtga ccgactatgc cgacaacttc
    31381 tacgagcctg cgatgaaaga tgcggttgcg cttcgcggct ccaactaccg tcccttgttt
    31441 gctctcgccg agctggccga agcgattcgc ctgcgctggc ccgagatcgc gatggacagc
    31501 gtgcgattcg gcggcctcgg aagcgacgag ttgctcgttg gaacggtcgt atctgccgag
    31561 ctgtcgcttc atcacccggg gctggatgct agagacctcg acgtggaagc cgtggtgaca
    31621 ttcggaagcc gtctacacga cctgtcctcg cgaatcgtca tcccgtttga aacggatggc
    31681 tcggacgacc gaagcacttg gcgaggcgca tttaccgtgc gcgccagcgg cggccacgag
    31741 ctgaggttca gagtgaggcc caaggaccga agtccgctcc ggcccgcagc gttgggaatg
    31801 cacaaccaga agtggctgta gacagccgcc gacgccagcg ctgaacgaac ggtgcgcgcg
    31861 ctagtgtcag aggcagaagc agcggcaggt tcacacaggc accggcactg acaccctggc
    31921 tcacgtggat gcggacggag gaggacggat cgcgatgggc agcgcagcga ttgggtcgag
    31981 cctagccgta ggtcatctta gagaactggg ggcgagtggg tcccaatcgc gagcacgccc
    32041 gtcgcgagcc gtcctccggt gacgcatctg cgcaacacag tggctggcga gggtgtcaga
    32101 ctatggcgcg ggcgaagtcc aggcccccga tgatgccggt gatcccgtgg tcgttttcga
    32161 tcagtattcg tcggacgttc attccgaggg cttgctcggc ggcgcggccg agagccatgc
    32221 cttcaggcag aatcaggatt cgaacgttca tcgcgtgctc gaccgccgtg tttggaggca
    32281 ggcggcggga ttctagggcg ctggcctgat cgaagactcc gaccggaaat tttccttcga
    32341 cgaccacgag tccgtgcttg ttggacagat cgagacggtc gacagccatg gcgatcgggt
    32401 catcggcttt gaccttcacc accgagccag acgcgaattc ggagatcggc gtctttatgc
    32461 gcttctctcg gacggcgcgc atcaaatcgc gagtggagac gacgccttcg ggcttgccgc
    32521 caactgtcac gaaaattcga tgcacgcggt gcttcagcat cagcttggcg gcttccgtca
    32581 gtggcgcgtc cggagacacc tgaatcgcgg gcgactgcat gagctcatcg acggaccggt
    32641 cgggcactcg gaaggtctcg ccctgtgtga agacggcaga atgcaggatg tcagttcgcg
    32701 aaaccacgcc cttgagctca ccctctgcat tgactacagc caacgcggac acgccttgct
    32761 cactgaacag gccgttcgcg gcaattaggc tttcgccgat gccgattgtt tcgaccgggg
    32821 tcgacatgta ctcgctcacg ggcatttcga aattagccat cgggtcttcc tcttcaggtg
    32881 gtgcgctcgc cgaacgagca actagtcaaa gggcgataac gtcgctgagg cgctcgacta
    32941 gctcgctcgc ctcgattgcg gcgaagtctt tgtcgagtga gcccacaacg agttgagacg
    33001 tcggaccatc tagagaagag cgcagcaagc ccaagaaacg gggtggcgcg acgagcacca
    33061 gttgcgtgta tcgattcgtg acacggccat gctcgagctt ggctgcgact gctctcgcaa
    33121 aatccgaaac tgcccggtcg tgcgggctct cgctctgccc catcgaagaa cgacgtggat
    33181 cgccgctgtt cttttgaaag gcacgcccag gacgatcgga agccatttca ctgtcccgcg
    33241 ccctgcccgc ggaatgctcg atttcctcca cgagcgctaa tccttgccca gcgccgtcgc
    33301 tctcgaagaa gcgcgcccct gcatcgtgcg ccaccacaac ccatgtcttg aacatcatcc
    33361 acctccctag ctgtaactca ggacgctggc aacgagggga gaggccggag ctcgatgatc
    33421 tgcttcgagt cgtcctcgcc gacgacctcg acgctcgtaa gcatccctga ttgctcgcgt
    33481 acgtagattt ccttcgggct cggcacacga tgggagatgc cgggcgtcgc gacttcgaag
    33541 acctggtcgt tcgagtcgta gctgatcccg atcagcgcgc tgttctcgct ttgaagctgc
    33601 acgccgatct cgtcgcctag gacggatacg cccacccgca tcgatggcag tctctttgcc
    33661 acatcgtcga agtacttctg ccaagttgac cgttctagtt tccgtgtcgt catctgcgcc
    33721 tcccggactg gaactaagaa tgtaagcaaa cgccgtgccc atccatgaga gccactttcg
    33781 gccctactgt tggcgcgagg cgcaaacggt gcgtggaatg agcctcgggc gacagaaatg
    33841 cgtgggactt ctggctccta ggggtgagag agccgcgccc gcttggtata gtcacgaacc
    33901 gcgggccgac tctgatggga aatctaaaag cggatctcaa agcagccgga ttcgagctga
    33961 tcgaaacgca catctcctgg gtgttcttgg gcgccagcga cgtcttcaag gtcaagcgtc
    34021 cggtcaacct cggctttttg gacttcacgt cactgcggca gcgacgggaa gcctgcgacg
    34081 cagagctccg gctgaatcga aggcttgcag ccgacgtgta tctcgacgtg gttccgatca
    34141 cccgcgacga gcttggtatt cacgcga
//
LOCUS       DASRZF010000413         4266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2382161, whole genome shotgun sequence.
ACCESSION   DASRZF010000413 DASRZF010000000
VERSION     DASRZF010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2382161"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VFG22_18390"
     CDS             <1..317
                     /locus_tag="VFG22_18390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418272.1"
                     /translation="KIVEATLFSLQAIQENPVLRQVWQPATFDASVLHGFTQPASITF
                     TRIALSEMIEASGWDDAESTEAMEFMLRMILSLLASPEPRRSEQDLRAFLRRRLVPGL
                     AL"
     gene            331..1341
                     /gene="dctP"
                     /locus_tag="VFG22_18395"
     CDS             331..1341
                     /gene="dctP"
                     /locus_tag="VFG22_18395"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HET6418273.1"
                     /translation="MRTWKHTLVVGLALAALFLPGSAASSATADGPIVIRYASLAPPG
                     SAFGKILKAWGRQFKKETEGRAELRFYTGGSQGDERDFIRKIRAGQIDAAGITTTGMG
                     MVVRPILVLTAPGLITEMAQLYYIRDRLRERFETMFRDAGFELLTWGDGGKNRLFSTE
                     PFLRPEDLKRGRPWAWKDDPVFAGYLGVIGANPVRVGANEVYGGLQTRMIDTVPSSCI
                     MAVAIQWYTKLNYMAKQNFNIIIGGSIIKKEVFDQLTPGDQKILLDTAERGARAMDKI
                     VIRDDERAYQTLIERGITEVDLTPYQAEWDAVAKKAREQLAGRVYSKSLLDEVTKLAA
                     EK"
     gene            1379..2392
                     /gene="dctP"
                     /locus_tag="VFG22_18400"
     CDS             1379..2392
                     /gene="dctP"
                     /locus_tag="VFG22_18400"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HET6418274.1"
                     /translation="MQHRKFPLILALTLAALFVPGRPAVSQTPAAPKTTIRIASLAPP
                     GSSFVKVLQAWSRTLEKETEGRVELRVFSGGSEGSDRDLVQRIKAGKLDAAGVATTGL
                     SMVAPELQVLTAPGLLIDDAQLDRAREKLDAKLQGLIEDGGFKLLAWGDGGKNRVFSS
                     TEFRSPADLGGKPAWAGKENAVALEYVKALGANPVRVGASAVFPSLKDGKLEVVPASA
                     MAAVAFQWYTKLNYMSTSNFNIIVGGSIISKKKFNELNAADQKTLLDTAKRAASKLDE
                     IVGRDDARAYQTLLGRGISEVDTSANKAAWDAAAKQARERLAQQGVYSKSLLKAVETA
                     AQN"
     gene            2409..3980
                     /locus_tag="VFG22_18405"
     CDS             2409..3980
                     /locus_tag="VFG22_18405"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HET6418275.1"
                     /translation="MTPECILAIDLGTSGPKVALVTTDGDVLASEVESTELFLSDGGG
                     AEQDPDDWWRAIVAATRRITDRNLVPAEQILAVAVTSQWAGTVPVDQDGNALSRAIIW
                     LDSRGAKYVPEVAGGLIRIQGYGALKLESWIRKTGGVPSLAGKEPVAHILFLRHERPE
                     IYEAAYKFMEPKDYLNLRLTGKFLATTDSIALHWVTNNRDIDNIHYDDQLLAQTTLPR
                     EKLPDLARAVDVIGTLTPKAADELGLKQDTKVVGGTPDVPAAAVGSGAVGDFEGHIYV
                     GTSSWLTCHVPFKKTDIIHNMASLPSALPGRYFIGNEQETAGACLTWLRDDVLCRDDV
                     LDPGPPPDDVFERFGLLAAETPAGADKLIFTPWLYGEHTPVADPYLRAAFSNVSLRTT
                     RAHMIRAVMEGVSYNTRWLMGGVESFIGRPFEALRFIGGGAKSDLWCQIFADVLDRPI
                     DQVADPLSANVRGAAFVAAVGLGKLKVEDIASRVPIEKRFVPNQAHRSIYDELYKAFL
                     EIQKNSAAMYKRLNS"
     gene            4030..>4266
                     /locus_tag="VFG22_18410"
     CDS             4030..>4266
                     /locus_tag="VFG22_18410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418276.1"
                     /translation="MDIKDLASIRLGPLEPLAARIYRYAKRFPFVKEKLEAEYAPIME
                     ELRKSAKPYAGEVESHALLPKDGKARTDILDEIRQ"
BASE COUNT          862 a         1322 c         1340 g          742 t
ORIGIN      
        1 acaaaatcgt cgaggccacc ttgttcagcc tgcaagccat tcaggagaac ccggtccttc
       61 ggcaggtttg gcaacctgcg acgttcgacg cgagcgtctt gcatggcttc acgcagccgg
      121 cgtcgatcac cttcacacgc atcgcgctga gcgagatgat cgaggcgtcg ggctgggacg
      181 acgccgaatc cactgaggcc atggaattca tgctcaggat gatcctttcg ctgctcgctt
      241 ctccggagcc tcggcgaagt gagcaggatc tcagagcttt cctcagacgg cgattggttc
      301 cgggcctcgc cttgtagtaa ggaacccgca gtgagaactt ggaagcacac actggtggtt
      361 gggctcgcgc tggccgccct tttcttgccc ggaagcgcgg cctcgagcgc caccgccgac
      421 ggcccgatcg tcatccgata cgcttcgctg gcccctccgg gctccgcgtt cggcaagatc
      481 ctcaaagcct ggggccgtca gttcaagaaa gagaccgagg gtcgcgcgga gttgcgattc
      541 tacaccggag gcagtcaggg cgacgagcgc gacttcattc gcaagatacg cgcggggcag
      601 atcgacgccg ccgggatcac gaccaccgga atgggtatgg tcgtgcgacc gattctcgtc
      661 ttgacggcgc cggggctaat caccgagatg gcacagctct actacattcg agatcgactt
      721 cgagaacgct ttgaaacgat gttccgagac gcgggcttcg agctgctcac ctggggcgat
      781 ggtggcaaga atcgtctctt ctcgaccgag ccgtttttgc gtcccgaaga cctcaagcgc
      841 gggaggccct gggcctggaa ggatgatccg gtgttcgccg ggtacctggg cgtcatcggc
      901 gccaatcccg tgcgcgtggg tgcgaacgaa gtttacggtg gattgcaaac gcgcatgatc
      961 gacacggtcc cctcgtcctg catcatggcc gtcgcgatcc agtggtatac caagctcaac
     1021 tatatggcga agcagaactt caacatcatc attggtggat cgatcatcaa gaaagaggtc
     1081 ttcgatcagt tgacaccggg ggaccagaag attctgctcg ataccgctga gcgcggcgcg
     1141 cgcgcgatgg acaagatcgt gattcgcgac gacgagcggg cctaccagac actgatcgag
     1201 cgcggaatca cggaggtcga cctgaccccc taccaggccg aatgggacgc tgtggccaag
     1261 aaggcgcgcg agcagcttgc cggccgcgtc tacagcaaga gcttgctcga cgaggtcacc
     1321 aagctagccg ccgagaaatg aacaactgcg gcctccggcc gtccaaccgt gagccgatat
     1381 gcaacaccgc aaattcccgc tgatcctcgc tctgacactc gccgcgctgt tcgtacccgg
     1441 gcggcccgct gtctcccaaa ctcctgccgc tccgaagacc accatccgga tcgcgtcgtt
     1501 agcgccgcca gggtcttcgt tcgtcaaggt tctccaggcc tggagtcgga ccctcgaaaa
     1561 ggagacggag ggtcgggtgg agctccgcgt cttttcgggc ggaagtgagg ggagcgaccg
     1621 cgacctcgtc cagaggatca aggcgggtaa gctggacgcg gcaggtgtgg cgaccaccgg
     1681 tctcagcatg gtcgcgcccg agctgcaggt gctcactgcg cccggactct tgatcgacga
     1741 tgcacagctc gatcgcgccc gggagaagct cgacgccaag cttcaaggct tgatcgagga
     1801 tggtggattc aagctgctcg cctggggcga cggcgggaag aaccgcgtgt tctcctcgac
     1861 cgagtttcga tcgccggcag acctaggcgg aaagccagcg tgggctggca aagaaaacgc
     1921 ggttgctctc gaatacgtca aagcgctcgg cgcaaatccg gtgcgggtcg gtgcgtcggc
     1981 ggtgtttccg agcctgaaag atggaaagct cgaggtcgtt cccgcgtccg cgatggctgc
     2041 cgtcgctttc cagtggtaca cgaagctgaa ctacatgagt acgagcaact tcaacatcat
     2101 cgttggcggc tcgatcatca gcaagaagaa gttcaacgag ctcaatgcgg cggatcagaa
     2161 aacgctgctc gacacagcca agcgcgccgc gtcgaagctc gacgagatcg tcggccgaga
     2221 cgatgcacgc gcttatcaaa ccttgctcgg acggggtatc agcgaggtcg acacgagcgc
     2281 caacaaggcg gcctgggatg cggcagcgaa acaagcgcga gagcgactcg cccagcaggg
     2341 cgtctacagc aagagtctcc tcaaggccgt agagacggcc gcgcagaact agtcgggctg
     2401 aggccaccgt gacccctgag tgcattctcg caatcgacct cggaaccagt ggacccaagg
     2461 tcgccttggt cacgacggac ggtgatgtgc tcgcttcgga ggtggaatcg accgagctct
     2521 tcctcagcga tggcggaggc gccgaacaag accccgatga ctggtggcga gccatcgtcg
     2581 cggcaacgcg ccgaatcacc gaccgcaatc tcgtgccggc cgagcagatc ctcgccgtag
     2641 cggtgacctc gcaatgggct ggcactgtcc cagtcgacca ggatggcaat gcgctttcgc
     2701 gcgcgatcat ctggttggat tctcgggggg ccaagtacgt gcccgaagtc gccggcggat
     2761 tgattcggat acagggctac ggcgcgctca agctcgagag ctggatccgc aagaccggcg
     2821 gcgtcccgag cctcgcaggc aaggagccgg tcgcgcacat cctgtttctg cggcacgagc
     2881 ggcctgagat ctacgaagcc gcctacaagt tcatggagcc caaggactac ttgaacctca
     2941 ggctcaccgg gaaattccta gcgactaccg attccatcgc gcttcattgg gtgaccaaca
     3001 accgcgatat cgacaacatc cactacgacg accagctgct cgcgcagacg acgctgcccc
     3061 gagagaagct cccggacctc gcgcgggcgg tcgacgtgat cggcacgctg accccgaagg
     3121 cagcggacga gctgggcttg aagcaagaca ccaaagtcgt cggcggcact ccggacgtgc
     3181 cagcggcagc ggttggctcg ggcgcagtcg gggacttcga ggggcacatc tacgtcggga
     3241 cctcgtcttg gctgacctgc cacgtgccct tcaagaagac cgacatcata cacaacatgg
     3301 cgtcactgcc gtctgcgctt ccgggcaggt atttcatcgg caacgagcaa gagacggccg
     3361 gtgcctgctt gacctggctt cgggacgacg tgttgtgtcg cgacgatgtg ctcgatccgg
     3421 ggcctccacc agacgacgtg ttcgaacgct ttggtctgct ggccgcggag accccagcgg
     3481 gtgccgacaa gctgatcttc acgccgtggc tctacggcga acacacgccg gtggccgatc
     3541 cgtacctgcg agccgcgttc tcgaacgtgt cgctgcgaac gacgcgcgcg cacatgatcc
     3601 gcgcagtgat ggaaggggtt tcgtacaaca cccgttggct catgggaggc gtcgaatcgt
     3661 tcatcggccg ccccttcgag gcgctgcgct tcatcggcgg aggcgcgaag tcggatctct
     3721 ggtgccagat cttcgcggac gtgctcgacc gcccgatcga ccaagtcgcc gatccactct
     3781 cggcaaacgt ccggggtgcc gcgttcgtgg ctgctgtcgg actcgggaag ttgaaagtcg
     3841 aagacatcgc ttcacgagtc cccatcgaga aacgattcgt gccgaaccaa gcgcaccgct
     3901 ccatctacga cgagctctac aaggcctttt tggagattca gaagaacagc gcggccatgt
     3961 acaagcggct caacagctag cgtttcgaag aggctggccg atgcccccgt cctgcggtac
     4021 gcaagacgca tggacatcaa ggaccttgcg agcatccgcc tggggcccct cgaacctctg
     4081 gccgcgcgca tctaccgcta cgcgaaacgt ttcccgttcg tcaaagagaa gctcgaagcg
     4141 gagtacgcgc cgatcatgga ggagcttcgg aagtccgcca agccgtatgc cggcgaggtc
     4201 gagagccacg cactgctgcc caaggatggc aaagcgcgta cggacattct cgatgagatc
     4261 cgtcaa
//
LOCUS       DASRZF010000414         4804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_5126509, whole genome shotgun sequence.
ACCESSION   DASRZF010000414 DASRZF010000000
VERSION     DASRZF010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5126509"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VFG22_18415"
     CDS             <1..430
                     /locus_tag="VFG22_18415"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HET6418277.1"
                     /translation="PGFLPPTDNLNTTFAMAMIIFIVTHVYGVKEHGFVKYFSHFFGP
                     KVPWWIAWFINPLMFFIELISHIVRPITLAIRLMANMTADHLVLGIFVSLFAGMGLVL
                     LPVPVVFYLMGCLVITVQTLVFCLLSVIYITLAIQHEEEH"
     gene            471..791
                     /gene="atpE"
                     /locus_tag="VFG22_18420"
     CDS             471..791
                     /gene="atpE"
                     /locus_tag="VFG22_18420"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HET6418278.1"
                     /translation="MKKLALRLVSFAAPLALTATAFAQDAPMSEAEATAIAGRALAAG
                     LAIGIAAFGCGMGQGRAAAAALEGIARNPNASGKILVPLILGLALIESLAIYALVIAF
                     QVQP"
     gene            868..1377
                     /locus_tag="VFG22_18425"
     CDS             868..1377
                     /locus_tag="VFG22_18425"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HET6418279.1"
                     /translation="MSTEQDSTRAPDTSRKAGVPGAVKVGVVFIVLVGAAAWLLSGSD
                     NPFVYSKLVHEVMSEPDSFEGRRLRVEGELRQGSIEFREDPCEWRFVLYKEDKEMPVR
                     FPQCVVPDTFRDGMGIQVTVQGELGDDGVFLANQVIPRCPSKYEMQQRMEAGEEMPYD
                     PNAPLPGQS"
     gene            complement(1446..2006)
                     /locus_tag="VFG22_18430"
     CDS             complement(1446..2006)
                     /locus_tag="VFG22_18430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418280.1"
                     /translation="MRIVPALCIAFALIFGISTAAIPTASADAGDAVKSGAKKEASSA
                     KKEADAAKSDASKEAEKAKKEADKEAYRAKEKAIAAEDKAEKEAGKAKEKAETDAKAQ
                     KEAAKAKAEARTAEQEAKAASAKAARKERYLAQEEKRHETQLKQIEGRRARVIELHEK
                     QLRNIDAAEARENERYEKRVKQLKDQ"
     gene            2276..4567
                     /locus_tag="VFG22_18435"
     CDS             2276..4567
                     /locus_tag="VFG22_18435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET6418281.1"
                     /translation="MTWSHAVEPHRASSEPVPLGLAPGVARALEQRGIRELYSHQARA
                     FELASRGKHLVVSTPTASGKSLCYNLPVLQALADDPSATALYLFPTKALSRDQEEALR
                     GLIRDSDLSAGAITYDGDTPNDARRAARQRAGVLLTNPDMLHAGIMPHHTAWARLFSN
                     LKYIVVDELHTYRGVFGSHLSNVMRRLLRIARFHGSSPSFLFASATIGNPQEHASRIC
                     GAPVELIDESGAPTGPRQLVVYNPPVVNAELGIRQSYIKTAVRLTQDLVRAGVPTLVF
                     GNSRNGVEVMLKYLRDRLARDQIDPAAIHAYRGGYLPQTRRRIEGALRNGEIRCVVAT
                     NALELGIDIGALDAVVCAGYPGTMAGLWQRFGRAGRRQDLSLAVLVASSSPVDQYMAR
                     HPRQLISAPIEQARIDPDNIEILIQHLKCAAFELPFEPDDAYGDVPDDALAEALDYLA
                     DNGVVHPTSSAAGSIYHWASDSYPANNVSLRSASWDNFVIIDLEGNRTIAEMDWRSTH
                     TMLHEQAIYQHEGGQYQVERLDFDNHKAFVRHVKPDYYTTAMTHSKVAVLEQDQSATI
                     DLGEVPLDSGLGDVSVIEKVVGYKKIKFHTHENVGYGEVHLPEMQKHTSSFWLTFPED
                     FVQSQPEPRAVVVDALRGLSKAMHVVGAVGLMIDPRDLGRMLGSRNEDDAPPGKGQGP
                     GFDPTIFLYDTVAGGIGLASRLFEEREELLRRARRLIESCACDEGCPGCIGPDASGAD
                     EPEAARKRLVLGLLDAVGVSATH"
     gene            4574..>4804
                     /locus_tag="VFG22_18440"
     CDS             4574..>4804
                     /locus_tag="VFG22_18440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418282.1"
                     /translation="MSIKRNLARLSQSTPRHLDVASIRGALPGQRRETEHGELHLIDA
                     YLEPRHHHGRAPIAKALQVSTERLAQLALDPLL"
BASE COUNT          822 a         1536 c         1448 g          998 t
ORIGIN      
        1 tcccggcttt ctaccgccga ctgacaacct caacacgacc ttcgcgatgg cgatgatcat
       61 cttcatcgta acccacgtgt acggcgtcaa agagcacggc ttcgtgaagt acttctcgca
      121 cttcttcggg cccaaggtgc cctggtggat cgcctggttc atcaacccgc tgatgttctt
      181 catcgagctg atcagccaca tcgttcgccc gatcacactg gcgattcgtc tcatggccaa
      241 catgaccgcc gaccacctcg tgctcggcat cttcgtcagc ctgtttgcag ggatgggtct
      301 cgtgcttctg ccggtgcccg tcgttttcta cctgatgggc tgcctggtga tcacggtcca
      361 gactttggtc ttttgtttgc tgtcggtgat ttacatcacc cttgcgattc aacacgaaga
      421 agaacactaa gtttccctct cggaagagag gcttgaggag gctccggaaa atgaagaaat
      481 tggctctgcg acttgtcagc tttgctgcac cactcgctct cacggcgacc gctttcgcac
      541 aggatgcgcc gatgtcagag gctgaggcca ccgcgatcgc gggccgcgcg cttgccgccg
      601 gtctggccat cggcatcgca gcattcggct gcggcatggg tcagggtcgc gccgcggctg
      661 ctgccctcga gggcatcgct cgtaacccga acgcttcggg caagatcctc gtcccgctga
      721 ttctcggcct ggcgctcatc gagtcgctcg cgatttacgc gctcgtcatc gcatttcagg
      781 tgcagccgta agccataact cacgtcgggg cgtgctggtt ttggcgcgcc ctggcgggcg
      841 agggcttcga gcaccatact tgcgatcatg agcaccgagc aggattccac gcgagcaccc
      901 gacacctccc gcaaggccgg tgttccaggt gccgttaagg tcggcgtggt attcatcgtg
      961 ctcgtcggcg cggcggcttg gttgctttcg ggcagcgaca atccattcgt gtactcgaag
     1021 ctggtgcatg aagtgatgag cgagcccgac tccttcgaag gccgccgtct gcgggtcgaa
     1081 ggcgagctgc gtcagggctc gatcgagttc cgtgaagatc cctgtgaatg gcgtttcgtt
     1141 ctctacaaag aggacaaaga gatgcccgtg cgttttcccc agtgcgtcgt gcccgacacg
     1201 tttcgcgacg gcatggggat tcaggtcacc gtgcagggcg agctcggtga tgatggcgtc
     1261 ttcctcgcca accaggtgat tccgcgctgc ccctcgaaat acgagatgca gcagcgcatg
     1321 gaagccggcg aggaaatgcc ctacgacccg aacgccccgc ttcccggtca gagctagcca
     1381 gcaccagatg ccaaaaagcc cgcctcctca ctggagacgg gccttcggct tcgacatcgg
     1441 gcttgctact gatccttgag ctgcttgacc cgtttttcgt atcgctcgtt ctctcgggcc
     1501 tccgcggcat cgatgttcct gagctgcttc tcatgcagct cgatcacccg ggcgcgtcgt
     1561 ccctcgatct gtttcaactg agtctcatgg cgcttctctt cttgcgcgag gtagcgctct
     1621 ttgcgagcgg ccttggctga cgcggccttg gcttcctgct cggctgtgcg agcctcggcc
     1681 ttggctttgg cggcctcttt ctgagccttc gcgtcggtct cggccttctc tttggccttg
     1741 cccgcctctt tctcggcctt gtcctcggcc gctatcgcct tttccttggc tcggtacgct
     1801 tctttgtcgg cctctttctt ggccttctcg gcttccttcg aagcgtccga cttcgcggcg
     1861 tcggcttctt tcttggcgct gctggcttcc ttcttggcgc cgctcttcac cgcgtcgccg
     1921 gcgtcggcgc ttgcggtggg tatggcggca gtcgaaatgc cgaagatcag tgcgaacgcg
     1981 atacacaatg ctggaacgat tctcattctt ttcctcctct tggccgcgag cctcgacgaa
     2041 gtcgcccccg cccgcggtca ttgccaacct aggcccgaaa cctctctccc ccaagtggac
     2101 gccagcttct tgaccggcgc gactaaacaa ccgtacagtg ctgcgcatga gcgcagtggg
     2161 ggggcacaca agaccgcaaa atcagccgtt tcgaagggtc gatgagccct gggaacggcc
     2221 gcggggcacg ggcgaggttc tcgagcgctg gcggagcagt agcacttggc gaaacgtgac
     2281 ctggagtcac gcagtcgagc cgcatcgggc cagttccgaa cccgtgccgc tcggtttggc
     2341 gcccggggtt gcacgggccc tcgagcagcg tgggatccgg gagttgtaca gtcaccaggc
     2401 gcgcgcgttc gagctcgctt cccgcggaaa gcatctggtc gtctcgactc cgaccgcttc
     2461 ggggaaaagc ctttgctaca acctgccggt ccttcaagcg cttgcggatg acccgagcgc
     2521 aacggccctc tacctgtttc cgaccaaagc actgtcgcgc gaccaagaag aagcgttgcg
     2581 cgggctgatc cgcgattccg atctcagcgc tggggcgatc acgtacgatg gcgacacgcc
     2641 caacgatgcg cgtcgagcag cgcggcaacg tgccggcgtc ttgctcacga acccggacat
     2701 gctccacgcc ggcatcatgc cgcatcacac ggcttgggcg cgcctgttct cgaacttgaa
     2761 gtacatcgtc gtcgacgagc ttcacactta tcgcggcgtc tttggttcgc acctgtccaa
     2821 cgtgatgcgg cgcctcttgc gcatcgcgcg ctttcacggc tcgtccccga gcttcctctt
     2881 cgcctctgcc acgatcggca atccgcagga gcatgcgtct cgcatctgcg gcgcgccggt
     2941 cgagctgatc gacgaaagcg gcgcgccgac agggcctcgg cagctggtcg tgtacaaccc
     3001 acccgtcgtg aacgcggagc ttgggatccg gcagagctac atcaagaccg cggtgcgctt
     3061 gacccaggat ctcgtccgcg caggagttcc cacgctggtg ttcggcaact cgcgcaacgg
     3121 cgtggaggtc atgctgaagt accttcgcga tcgactcgct cgcgatcaga tcgacccggc
     3181 tgcgattcat gcgtaccgcg gcgggtatct tccgcagact cgccggcgca tcgaaggagc
     3241 cctgcgaaac ggcgagattc gctgtgtggt cgcgaccaac gcgctcgagc taggcatcga
     3301 catcggagcg ctcgatgccg tcgtatgtgc cggatatcca ggcaccatgg ccgggctttg
     3361 gcagcgtttc ggtcgagccg ggcgacggca ggatctctca ctggcagtgc tcgtcgcctc
     3421 gagcagtccg gtcgaccagt acatggcccg ccatccccgt caactgatct cggcaccgat
     3481 cgagcaggcg cgcatcgatc cggacaacat cgaaattctg attcagcatc tcaaatgcgc
     3541 cgcgttcgag cttccgttcg aacccgatga tgcatacggt gacgttccag acgatgcgct
     3601 cgctgaagct ctcgactacc tcgccgacaa cggagtcgtt caccccacgt cgagtgccgc
     3661 gggctcgatc tatcactggg catcggacag ctacccggca aacaacgttt cgcttcgaag
     3721 cgcgagttgg gacaacttcg tcatcatcga cctcgagggg aatcgcacca tcgccgagat
     3781 ggactggagg agcacgcata cgatgctgca cgagcaggcg atctatcagc atgaaggtgg
     3841 gcagtaccaa gtcgagaggc tcgacttcga caaccacaag gctttcgtgc gccacgtgaa
     3901 acccgactac tacacgaccg ccatgacgca cagcaaggtc gccgttctag agcaggacca
     3961 gagcgccacg atcgatctcg gagaggtgcc cctcgactct gggctcggtg atgtgagcgt
     4021 catcgagaag gtcgttggat ataagaagat caagttccac acacacgaaa acgtgggcta
     4081 cggcgaggtg catctccccg agatgcagaa gcataccagc tcgttttggc tcacgtttcc
     4141 cgaggatttc gtccagagtc agcccgagcc gcgggccgtc gtggtcgatg cattgcgcgg
     4201 tctttcgaag gcgatgcacg tggtgggcgc ggtcggcttg atgatcgatc cgcgcgacct
     4261 cgggcgcatg ctggggagcc gcaacgagga cgatgcgcca cccggaaagg gccaagggcc
     4321 cggcttcgac cctacgattt ttctctacga tacggtggcg ggtggaattg gcttggcgtc
     4381 acggctcttc gaggagcgcg aagagttgct gcggcgggcg cgccgtttga tcgagagctg
     4441 cgcatgtgac gagggctgtc ccggttgcat tggaccggat gcttcgggcg ccgatgagcc
     4501 cgaggcggcg cggaagcgcc tcgtgcttgg tttgctcgat gctgtcggcg tgagcgccac
     4561 ccactgagtt ccgatgtcga tcaaacgaaa cctcgcccga ctctcgcaat ctacgcctcg
     4621 gcatctcgat gtcgcgagca ttcggggcgc gcttccaggc cagaggcgcg aaaccgagca
     4681 cggagagctg cacctgatcg atgcttactt ggagccgcgt catcaccacg ggcgagcgcc
     4741 aatcgccaag gcgctccagg tctctaccga gcgactggct caactcgcgc tcgatccttt
     4801 gctt
//
LOCUS       DASRZF010000415         4799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4444919, whole genome shotgun sequence.
ACCESSION   DASRZF010000415 DASRZF010000000
VERSION     DASRZF010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 4799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4799
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4444919"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VFG22_18445"
     CDS             <1..320
                     /locus_tag="VFG22_18445"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HET6418283.1"
                     /translation="PPDQVAERLKRSIPDRSRVVALEVDGRALGSHDLAQWLGRAENE
                     SVQTVVFLIGGSYGLPQALSKQADLRLSLSAMTLPHRLARLFLAEQIYRGFSILRGEP
                     YDH"
     gene            complement(595..921)
                     /gene="cutA"
                     /locus_tag="VFG22_18450"
     CDS             complement(595..921)
                     /gene="cutA"
                     /locus_tag="VFG22_18450"
                     /inference="COORDINATES: protein motif:HMM:NF015077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HET6418284.1"
                     /translation="MKAENELILVLCTVPDEDTAQRLARGLVEARLAACVNAIPGVKS
                     YYRWKGKTEVDSEIQLLIKTQRTRFEELAAWIRANHPYEVPEIVALAADRVSESYLRW
                     ALEETS"
     gene            complement(935..1747)
                     /locus_tag="VFG22_18455"
     CDS             complement(935..1747)
                     /locus_tag="VFG22_18455"
                     /inference="COORDINATES: protein motif:HMM:NF024628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HET6418285.1"
                     /translation="MSRLIGYVANRADRMRDALYQERAVLRQESAQTDASGWGIGFYH
                     GDEVLHKKRPTRAGERVDWQSIAGDVHSDCAVLHMRQPTVGDFRAENAHPFRMRSWLF
                     AHQGTIGRFDAMRANLSEMIPDFIRRNIRGQTDSEYLFHTVLTLLHDAGQLDNPDVNQ
                     AQALPAISAAVTLVERLAAEVGSTELGLSFILTNGRSMYALRHGAPMSYVERQGLHDP
                     PDDYVPPPVGSTQLRYVMLVSGADEEPAGFESIPERAVAVVDRELGVTVHAL"
     gene            1911..2804
                     /gene="xerD"
                     /locus_tag="VFG22_18460"
     CDS             1911..2804
                     /gene="xerD"
                     /locus_tag="VFG22_18460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HET6418286.1"
                     /translation="MSAAFDGALDDYIQHLRVERGLSEHTVDGYARDLIRFGKWLVDE
                     RAALDEVDEARVAGYLVTLSQAGLSARTQARALSSIRGFFRFLVQERRQQLDPTELLE
                     GPRLLRKLPDVLNRDEVLRLLNAPIGVKPNRIRDRAMLHTMYAAGLRVSELVNLDLAD
                     LHLEEGFVSALGKGNKRRLVPIGDHARSALAEYLTEVRPRWAKPASRACFVTSRGKPM
                     TRQSFWSLVKRYARAAGITKPISPHKLRHSFATHLLSGGADLRSVQTMLGHVDISTTQ
                     IYTHVSGDHLRKMHERYHPRG"
     gene            2888..3550
                     /locus_tag="VFG22_18465"
     CDS             2888..3550
                     /locus_tag="VFG22_18465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418287.1"
                     /translation="MSSFDVWIIRFEPGTESPAKRLQAAFGMDAASARALEQSLPKIV
                     KHGIQAKAAGEMRQSLEAIGAVVECRPARDVKRVAGSKGSKEATIFHPPGEDLLPAGR
                     ISAIDPFESPSDARVPRISVDDVPLPAPRPRTAPPPEEPDVRRITSSLLATSREQQRR
                     QYAKQAVGSIVAGAAILGIGWFFGSSVLRGEADWIGIGFDGLGIYFIGAGVYDLVTTL
                     RS"
     gene            3556..4077
                     /locus_tag="VFG22_18470"
     CDS             3556..4077
                     /locus_tag="VFG22_18470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418288.1"
                     /translation="MQLTLPRILWFVAALAIAWGLNAWRREAASHGLKAAQRGDRSAA
                     ESWVDRALREGECELHEARGYLQSPVTRQYVRRPDYVESFVEKLYAGGAPKLEICDSD
                     LVGFRFAHYLLVTLPEDPRQQERVIADAQSFVRRDAVVYRGVSSTEVEAIVRDSTLVG
                     ERRVLLDLPAEAD"
     gene            4163..>4799
                     /locus_tag="VFG22_18475"
     CDS             4163..>4799
                     /locus_tag="VFG22_18475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET6418289.1"
                     /translation="MNIHEYQAKELYRKYGIPVPKGIACMSPKEATAAAEKLIQETGN
                     DVVVVKAQIHAGGRGKAGGVKVVRGVAAATAAANEIFGKTLITHQTGPEGKVVQRLLV
                     EQGLAIKSELYLALVVDRESRRIAIMASTEGGMDIEEVAAKTPEKIHTITIDPAVGLQ
                     GYQIRQLAFGLGLSKQEQKGFSKLLVGLAKLFEQEDCSIAEINPLILTEDEQ"
BASE COUNT          886 a         1451 c         1539 g          923 t
ORIGIN      
        1 gaccgcccga ccaggtcgcg gagcgactga agcgaagcat cccggatcgt tcccgggtag
       61 tggcgctcga ggtcgatggc cgcgcgctcg gcagtcacga cctcgcgcag tggctcggac
      121 gtgcggagaa cgaatcggtc cagacggtcg tcttcttgat cggcggctcc tacggtctgc
      181 cccaggcgct ctccaagcaa gccgacttgc gcctatcgct ctccgcgatg accttgcccc
      241 atcgcctcgc ccgcttgttc ctggccgagc aaatctaccg cggcttctcg atcctgcgcg
      301 gcgaaccata cgatcactag gtgagcgcgg atcgggcccc gggcgaacac gacacggggt
      361 tccatcgaac ggctcggctt gcaattgtgg ccggtgggac ttggggccgg gggccacgca
      421 agcctcgcct cggcgctcac gccaatcggg ggtggttgct ccctgaccga ttgtcattcg
      481 cgacgtcgcc ggaaccccgc atcgcgttcc gccccgcccc tccgtatgcc agtgccagtg
      541 ccagtgccag tgtcagcgcc agcgtcagcg tcagtgccag tgccagcgcc cggtctagct
      601 ggtctcttcg agggcccagc ggaggtagct ttcgctcacc cggtctgctg ctagcgcgac
      661 aatctctgga acttcgtacg gatggttcgc gcgaatccag gctgcaagct cctcgaagcg
      721 tgtgcgctgc gtcttgatca ggagctggat ctccgagtcg acctcggtct tgcccttcca
      781 tcgatagtac gacttcaccc cgggaatcgc gttcacacaa gccgccagcc gcgcttcgac
      841 caaaccgcgg gcgagccgct gcgcggtgtc ctcgtcgggc accgtgcaga ggaccagaat
      901 cagctcgttt tcggccttca ccgggtgcgc ctcgtcaaag ggcgtggacc gtgacgccga
      961 gctcgcggtc gacgactgca acggcgcgtt cagggatgga ttcgaatccg gccggctcct
     1021 cgtcggcgcc gctgaccagc atcacatacc gcagctgagt gctgcccacc ggcggcggca
     1081 cgtagtcgtc gggtgggtcg tgaagacctt gacgctcgac gtagctcatc ggtgcgccgt
     1141 gacggagcgc gtacatcgaa cgtccgttgg tgagaatgaa gctcaaaccg agctcggtgc
     1201 ttcctacctc tgcggcaaga cgctcgacca gcgtgaccgc tgcgctgatt gcgggcaggg
     1261 cttgcgcttg gttgacgtcc gggttgtcga gttgacctgc gtcgtgcagg agtgtgagca
     1321 cggtatggaa gagatactcg ctgtcggtct gaccgcggat gttgcgccga atgaagtcgg
     1381 gaatcatctc gctcagattt gcacgcatcg cgtcgaaacg tccgatggtc ccttggtgcg
     1441 cgaacagcca actgcgcatc cgaaacgggt gagcattctc tgcgcgaaaa tcacccacgg
     1501 tgggttggcg catgtgcaac acggcgcagt cggaatgaac gtcaccggcg atgctctgcc
     1561 aatcgacgcg ctcgcctgcg cgtgtcgggc gcttcttgtg aaggacttcg tcaccgtgat
     1621 agaagccgat gccccagccg ctcgcatcgg tctgtgcact ttcctggcga aggacggcgc
     1681 gttcttggta gagagcatcg cgcattcggt cggcccgatt cgcgacgtat ccgatgagcc
     1741 tactcatcgt ttgaacatcc aagcgaagtg taagtagagc atttcatcat gattcccctc
     1801 gttaatagat accacgcgcc tggatctgca tcaacgaact gaggtgctct ggggtcggat
     1861 gcgatcgtac acaaaatggg ctcggcgatc ggattgtggt agcgagtagt atgtccgccg
     1921 ccttcgacgg tgcgttggat gactacattc agcacttgcg tgtggaacgg ggcctttcgg
     1981 aacacacagt ggatggctac gcccgcgatc tcattcgctt tggcaaatgg ttggtggatg
     2041 agcgagccgc gctcgacgag gtcgacgagg cgcgcgtggc cggctatttg gtcacgctat
     2101 ctcaggcggg cctgagcgcg cggacgcagg cgagggccct cagctcgatc cgtggattct
     2161 ttcgcttcct ggttcaggag cggagacagc agctcgatcc taccgagttg ctcgagggcc
     2221 cgcgcttgct caggaagctg cccgacgtgc tgaatcggga tgaggtcctt cgacttctga
     2281 acgcgccgat cggggtcaaa ccaaaccgaa tacgcgatcg ggcgatgttg cacacgatgt
     2341 atgctgcggg gcttcgtgtg agcgagttgg tgaacttgga tctggccgat ctccatctcg
     2401 aagaggggtt cgtttcggct cttgggaagg gcaacaagcg acgtcttgtt cccattggag
     2461 atcatgcgag gtcggcgctg gcggagtacc tcaccgaggt caggccacgt tgggcgaagc
     2521 cggcgagccg tgcttgcttc gtcacttcgc gtgggaagcc gatgacgcgg cagagtttct
     2581 ggagtttggt caaacgctat gcgcgcgccg cgggcatcac caagccgatc tcaccgcaca
     2641 agctacgcca ctcgttcgcg acgcaccttc tgtccggcgg agcagacctg cgttcggtcc
     2701 agacgatgct tgggcacgta gatatctcga ccacccagat ttacacccac gtgagcgggg
     2761 accatctgcg aaagatgcac gagcggtatc atcctcgagg gtagtcgcta tgatcacaag
     2821 ttcaggttcg cgggcggttt ccttcaagta atcgcccgac cgcggccgag tcggtatcct
     2881 ccggggtatg tcgagcttcg acgtttggat cattcgcttc gagccgggca ccgaatctcc
     2941 ggcgaagcgc cttcaagcgg cgtttggaat ggatgccgcg agtgctcgtg cgctcgagca
     3001 gagcttgccc aagatcgtca agcacggcat tcaagcaaaa gcggctggtg agatgcgcca
     3061 gagtctcgaa gcgataggcg cagtcgtcga gtgtcgaccg gcgcgggatg tgaagagggt
     3121 cgccggttcg aaaggttcca aagaagcgac aatcttccat ccccctgggg aggacctgct
     3181 accggccggt cgcatctccg caatcgaccc tttcgagtcc ccttcggacg cccgcgtgcc
     3241 gcgcatctcc gttgatgatg taccgctgcc cgcgcctcgg cctcgaaccg caccgccacc
     3301 ggaggaaccc gatgttcgcc gtattaccag ctcgctactg gcgacttctc gcgaacagca
     3361 gcgccgccag tacgcgaaac aggcggtagg cagcatcgtc gccggcgctg cgatcctggg
     3421 catcggctgg ttctttggaa gctcagtgct tcggggagag gccgactgga ttggcatcgg
     3481 cttcgatggt ttgggcatct acttcattgg tgccggggtt tacgacctcg tcacgacgct
     3541 gaggagctag cttccgtgca actgacgctt ccgagaattc tctggttcgt agccgcgctc
     3601 gccattgcat gggggctcaa cgcatggcgc cgagaggccg cttcgcatgg cctaaaagcg
     3661 gcgcaacggg gagatcggag cgccgcggag agctgggtcg atcgggccct ccgagagggc
     3721 gagtgtgagc ttcatgaggc gagggggtac ctccaatcgc ccgtgacgcg gcaatacgtg
     3781 cgccgccccg actatgtcga gagtttcgtc gagaagctct acgccggcgg tgcccccaag
     3841 ctcgagatct gcgattcgga tctggtcggc tttcgtttcg cgcactatct attggtcaca
     3901 ctgccggagg atccacgcca acaagagcgg gtcatcgccg atgcccagag cttcgtgcgc
     3961 cgcgacgcgg tggtgtatcg cggcgtgagc tccacggaag tcgaggcgat cgtgcgcgac
     4021 tcgactctcg tcggggagcg ccgagtgctc ttggatctgc cagcagaagc tgactagagc
     4081 acgattcggg cgcctccgtc gactcgtgct atagccctgc cgctctgcac gcaatagaga
     4141 cgacgttgag gaggcaagca ccgtgaacat tcacgagtat caagcaaaag agctctatcg
     4201 aaagtacggg attcccgtac cgaagggcat cgcctgcatg tcgccgaagg aagcgacagc
     4261 ggcagccgaa aagctcatcc aagagaccgg caacgatgtc gtcgtggtga aggcgcagat
     4321 tcacgcaggt ggacgaggca aggccggtgg cgtgaaggtg gtcaggggcg tcgcggcagc
     4381 gacagcagcg gccaacgaga tattcggaaa gaccctgatc acgcatcaaa ccggtccgga
     4441 agggaaggtc gtgcagcgcc tcttggtcga gcaggggctc gcgatcaaga gcgagctcta
     4501 cctcgcgctg gtcgtcgatc gcgagagtcg tcgaatcgcg atcatggctt cgaccgaagg
     4561 cgggatggac atcgaggaag tcgccgccaa gacccccgag aagatccaca ccatcacaat
     4621 cgaccctgcc gtcggcttgc agggctacca gattcggcag ctcgccttcg ggctcggcct
     4681 ttcgaagcag gaacagaaag gcttctccaa gttgctggtc ggtctggcga agctcttcga
     4741 gcaggaggac tgctcgatcg ccgagatcaa tcctctcata ctcaccgagg atgaacagg
//
LOCUS       DASRZF010000416        11691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2045076, whole genome shotgun sequence.
ACCESSION   DASRZF010000416 DASRZF010000000
VERSION     DASRZF010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 11691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11691
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2045076"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VFG22_18480"
     CDS             <1..517
                     /locus_tag="VFG22_18480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418290.1"
                     /translation="MKVVVLNPGGVAGPYDANTWGQLFFLLRDNALPAASPGTTTISH
                     VHDIVAAHIAAVTKGRNGERYILASDDVELVDLIGEVARVSGIAKAPKKAPPFVIKAF
                     GRLSVIVAAITGKEPDVTPEIAAATSARGTSFSSEKAIRELGYRIQPMEVAVKDNYDW
                     LVKEGLLGQSG"
     gene            501..1514
                     /locus_tag="VFG22_18485"
     CDS             501..1514
                     /locus_tag="VFG22_18485"
                     /inference="COORDINATES: protein motif:HMM:NF021285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HET6418291.1"
                     /translation="MANLDDMAGVLVRPLQLLRSRPRNTLDRLVAYEGLRQDQLFPAP
                     ERLPRVRTRPRFSLPGFENEDLSFASLHEPIEPLFKEHYHARSRRIQTVYARRIRPKG
                     AEGRPRILYIHGYMQPETVVEEHGLLGVIARTLNVELVQLQPPYHGRRKPRSSPYDGE
                     RFWTADIVRSFEAMRQTIIDARTLLSIMLEESPTPVGVAGLSLGGSFSAILACLEPRL
                     FFSVPLIAHMDMGALFRDAPVLGAMRKDLARFGWGPRHFGQFFTRIGWDDLTPVIPAS
                     RMLLVAAEKDRFFDAEVLRKMWSRWDEPEIHWYPTSHMGFIPHMPSAIGHMRKFVNRI
                     SRS"
     gene            complement(1501..2361)
                     /locus_tag="VFG22_18490"
     CDS             complement(1501..2361)
                     /locus_tag="VFG22_18490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418292.1"
                     /translation="MKSRFSCFFPRAFLVLGLVSSSYLLGCKADALSEGGTTVVMLYA
                     EPVGCENLGVVIGRGGGLTGAYSKPRVNQESAENDALNEAAELGATHLLLHPEEVAQG
                     DGHQPTEQDTEPAMAHGYGTGSTVTVAGTAYKCAPGTLPPATATSIHAGSAVAESRES
                     QVPRSISLAPLGALKSITVFHRGPLASGTGVGETEVRKVQDPAEIERVVASLKQVAED
                     PMKYIPTDRVEFVGELGVQSLLYGFGYLQYAGGVYRLTTADFEEVLRLREEPPPSEAT
                     PESGEGSTSD"
     gene            2490..4376
                     /locus_tag="VFG22_18495"
     CDS             2490..4376
                     /locus_tag="VFG22_18495"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA synthetase"
                     /protein_id="HET6418293.1"
                     /translation="MSATVETRHRSLEDPEGFWGEVAEGIDWISRWDRVLDSSNPPFY
                     RWFPGAQINTCYNALDRHVEKRGNQAALIYDSAVTHQRRTYTYRQLRDDVARFAGALA
                     SQGVTKGDRVLLYMPMIPEAVIAMLACARIGAVHSVVFGGFAPQELATRIDDAEPKII
                     VSASCGIEGTRVIEYKPLLDQAIDLSEHKPSTCIIRQRESCECDLTEGRDISWQDAVA
                     AAKPHDCVPVLATDPLYILYTSGTTGIPKGVVRDHGGHAVALQWTMRNVYGVEPGDVY
                     WAASDVGWVVGHSYIVYGPLLNGNTTILYEGKPVGTPDAGAFWRVIEDHGVNCLFTAP
                     TAFRAIKKEDPNGLLLADYDLSTLRSLFLAGERSDPDTLKWAEDKLGVPVIDHWWQTE
                     TGWAICANCLGIEHLPVKHGSPTVAVPGWDVQVVDEACHQLKPGKTGALVVKLPLPPG
                     SLPTLWNDDERFVKSYMTEFPGYYKTADAGYIDEDGYVYVMSRTDDIINVAGHRLSTG
                     GMEEVVAAHPDVAECAVIGVADQLKGEIPLGFLVLNAGVDRPQHDIVSEVIEMVRNAI
                     GPVAAFKTAIVVQRLPKTRSGKILRGTMKKIADRSPWKTPATIDDPVILDEIRERLQA
                     IGYA"
     gene            4377..4544
                     /locus_tag="VFG22_18500"
     CDS             4377..4544
                     /locus_tag="VFG22_18500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418294.1"
                     /translation="MNREQERALLLFGLAVVLIATPVRAIWLQTWWSPFAFWLSMIAI
                     IALLHRIDHAA"
     gene            4534..7401
                     /locus_tag="VFG22_18505"
     CDS             4534..7401
                     /locus_tag="VFG22_18505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6418295.1"
                     /translation="MQLNHFVLFFLAAALLGLYILGSEVVERSPRWGRLGRNRFSFAL
                     ALGVYATTWTLFGSVGFAATEGYDFLAIYFGVTLSCIAIPFLWAPVAELVDRYRLGSI
                     ADLFAFRYQSRTLGAAVSIFMVAGLLPYIALQLRAVADGAAILVGQEPSEELGSVYAI
                     LLSAFAVALGVRYADGRAHRPGLIATLAFESIFKLVALLVVGAATLLTVFGGLRGLNE
                     YLRDRPELVESMTDPVMGDSWIALVFVTFCAAFLLPRQFFVAFVQRPGPRSLETARWA
                     LPLYLFAINLPVPILYWAGRDLASTGIRPDLYVLVAVQSSPALQTLAFLGAVSAASAM
                     ILVSSIALSGMVANYLVPRFGPSPESLLTRWAVFRRSTIVGVVLAGLLIHFTLPRTRS
                     LVDIGLASFVAVTHLIPGLLGALFWRRATRSGVLAGLIVGATSWAVLTASPLFGLELD
                     ASRLYALFGFTDPSARGPAIWLSLTAHGFMFIGVSLLTDRSAEERNAAEACREPRVTR
                     LLQLPDSPEALQERLTSYVGKETAIREIYEAAAAADVYWPPRDRPELSRLIAELEGRL
                     SERIGPLAARTYVQGGRPVRADALAERLRAFRELDSLDADASQRSSPAHAARRYLSQL
                     MSELPVGVCTVDEHEDVVVWNAQLERMSGLAEDEICGNPIHDIPSPWWPLFDELAHQP
                     TGSVIEREYNLGDRNIDLRAQTATIPGGGEDGRVFVVDDITALRQLERHVAHKDRLSS
                     IGTLASGVAHEVGNPLTGILMVAKNLASDPGAEDAAERLGIIIHEAQRIHGIVQSLLT
                     FSRRDESPLEVQTISVAAVVRAAAELAKLETQGCRIDLELAVDATAMASSDGLTQVMV
                     NLLNNARQACPAGATVCVRTRLDPERVYIDVDDEGPGIPEDLSERVFEPFFTTKSDGQ
                     GTGLGLSVSRQIVERFGGTLSYEPRETGGSRFTITLKAFEN"
     gene            7442..8776
                     /locus_tag="VFG22_18510"
     CDS             7442..8776
                     /locus_tag="VFG22_18510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6418296.1"
                     /translation="MAKILLVEDERVIRAEIRRILTRDGHEVRDVASVPAAEQERPES
                     FDLVITDVRLTGDTGDVLIERVGQVPVIVMTAYGSVGAAVDAMKRGAADYLSKPFEPE
                     ALSAAVDRVLRHRASSSTRDVDPAFDPFAIVTTESSPMQAALRCARKVAPTEATVLIL
                     GESGTGKELIARSVHNQSARTGRFVPVNCASIPETLLESELFGHEKGAFTGALARHDG
                     LVHAANGGTLFLDEIGELSHAAQARLLRMLQQREVRRVGAQIAEAVDIRLIAATHRNL
                     PTLVERQEFRADLYFRLKVVEIQLPPLCERGDDILALAEVFIDRAAEKLGRRPLELSP
                     GAAAAIERYKWPGNVRELQHAVERAVILHESGPITEDLLGLPRSTPPEHVANGAGGED
                     PSLEGYLRRFVLSHQDELSETELAKRLGISRKTLWERRQRMGIPRRESRPPE"
     gene            8936..10852
                     /gene="acs"
                     /locus_tag="VFG22_18515"
     CDS             8936..10852
                     /gene="acs"
                     /locus_tag="VFG22_18515"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HET6418297.1"
                     /translation="MTLTQPSLDSRKHITSLEEYETLYQRSLSDREEYWLAEGNELHW
                     FYEPHSVFEEHGPGEFVWFAGGRLNATINCVDRHADKTPDKTAIIFVKDEPGEYEHIS
                     FAQLKTAVGRIANSLKSMGVQKGDRVCIYLPMVPELAYTMLACARIGAVHSIVFGGFS
                     ADSLRDRIIDAQAKVLVTANEGLRGGKRIPLKKICDDAVEGLAVVEKVLVVKRTDTEV
                     NMEHDRDVWMHEAMAEQRVTCPAEWMASEDPLFVLYTSGSTGKPKGLVHTTAGYLTFA
                     ASTFKYVFDIQPDDIHFCAADIGWITGHSYIIYGPLANGITTVMFESVPTYPDASRYW
                     KVIDDIGATTLYTSPTALRALMREGSEPVQGSSRKSLRVLGTVGEPINPEVWRWYSDV
                     VGNGKLPIVDTWWQTETGGILISPLPNATPTKPGSATFPFFGIEPLLVDQDGKEIEGN
                     GVSGYLCIRHPWPGQARTIHGNHKRFIETYFSQYPPFYFTGDGCRRDEDGYYWITGRV
                     DDVINVSGHRMGTAEVESALVAHQAVAEAAVVPFPHEIKGQGIFAFVVPISGAEWEPH
                     ELQGALKEQVRQVIGPIATPDEIRVVTGLPKTRSGKIMRRILRKIAAGEGDQLGDLST
                     LADPSVVEALLKKV"
     gene            10885..11184
                     /locus_tag="VFG22_18520"
     CDS             10885..11184
                     /locus_tag="VFG22_18520"
                     /inference="COORDINATES: protein motif:HMM:NF025305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HET6418298.1"
                     /translation="MATESEELRSSKHTQDVADRYWKRNLRILFVLLAIWWGVSFGAG
                     IIWAGSLDNITIPGTGFKLGFWFAQQGSIYVFVVLIFVYTRLMNKLDKEFDVHED"
     gene            11212..>11691
                     /locus_tag="VFG22_18525"
     CDS             11212..>11691
                     /locus_tag="VFG22_18525"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418299.1"
                     /translation="MDVQTWTFIIVGGTFALYIGIAIWSRASTTGDFYIAGRGVHPIV
                     NGMATGADWMSAASFISMAGLISFMGYDGAVYLMGWTGGYVLLALLLAPYLRKFGKFT
                     VPDFVGDRYYSNSARIVAVLCAVFVSFTYVAGQMRGVGVVFSRFLEVDINVGVLIGMA
                     "
BASE COUNT         2207 a         3627 c         3552 g         2305 t
ORIGIN      
        1 tatgaaggtc gtcgttctga atcctggcgg cgtggcaggc ccttacgacg cgaacacctg
       61 gggtcagctt ttcttcttgc tccgggacaa cgcgctccct gccgcatcgc cgggaacgac
      121 gaccatttcg cacgtgcacg acatcgtcgc tgcgcatatc gccgccgtca cgaaaggtcg
      181 aaacggagag cgctacatct tggcgagcga cgatgtcgag ttggtcgatc tgatcggcga
      241 ggtcgcgcgc gtctccggga tcgcaaaggc accgaagaag gcgccgccgt ttgtcatcaa
      301 ggcatttgga aggctctcgg tcatcgttgc cgccatcacg ggcaaggagc cggacgtgac
      361 cccggagatc gccgccgcga cgagcgcaag gggcacgagc ttctcgagcg agaaggccat
      421 ccgtgagctc ggataccgaa tccagcccat ggaagtggcg gtcaaagaca actatgattg
      481 gcttgtgaag gaaggtctgc ttggccaatc tggatgacat ggcgggcgtc ctcgtgcgcc
      541 ccttgcagct cctgcgatct cgtccgcgaa atactctgga tcgactggtg gcgtatgaag
      601 ggcttcgcca agaccagctc ttcccggcgc ccgagcgact gccccgggtt cgcacgcgcc
      661 cccgattctc gctgccaggg ttcgagaacg aggacctcag ctttgcttct ttgcacgagc
      721 cgatcgagcc tcttttcaag gagcattacc acgctcggag ccgccgtatc caaaccgtgt
      781 acgccaggcg catcagaccc aagggggccg aggggcgccc gcgtatcctc tacatccacg
      841 gttacatgca gccagagacc gtcgtcgaag agcacgggct cctcggggtg atcgcccgga
      901 ccttgaacgt agagctcgtc cagcttcaac ctccttacca tggccgtcgc aagccccgaa
      961 gctcgccata cgacggcgaa cgcttctgga ccgcggacat cgtgcgaagc ttcgaggcga
     1021 tgcggcagac gatcatcgat gcgcgcacct tgctgtcgat catgctcgag gagtcgccca
     1081 cgccagttgg cgtcgccggg ctgtcacttg gagggtcgtt ttccgcgata ctcgcttgcc
     1141 tcgagccgcg tctcttcttt tccgtgcctt tgatcgcgca catggatatg ggcgcgctct
     1201 tccgtgacgc gccggtgctc ggggcgatgc ggaaggacct cgcgcgtttt ggctggggcc
     1261 cgcggcattt tggtcagttc ttcacacgca ttggttggga cgatctcaca ccggtgatcc
     1321 ccgcaagccg gatgctgctc gtcgccgcag agaaggaccg cttcttcgat gcggaagtgc
     1381 tccggaaaat gtggagtcgc tgggatgagc ccgaaatcca ttggtatccc acgagtcaca
     1441 tgggattcat tccacacatg ccgagcgcga tcggccacat gcggaagttc gtaaaccgca
     1501 tcagtcgctc gtagagcctt cacccgattc gggcgtagct tccgacggag gaggctcctc
     1561 tcggagcctc agcacctctt cgaagtcagc ggtggtcagc cggtagacac cacctgcata
     1621 ttgaagatac ccgaagccat agagcagcga ctgtacgccg agctcgccga cgaactccac
     1681 tcggtccgtg gggatgtact tcatggggtc ttcggcaacc tgcttcaaag aagcgactac
     1741 ccgttcaatc tcggcaggat cctggacttt ccggacctcg gtctcaccca caccagtccc
     1801 cgaagcgagg gggccccggt ggaacacggt gatgctcttc agcgcgccga gcggcgctag
     1861 agatatcgat ctcgggacct gcgactctcg ggactcggcg acggcgctgc ccgcgtgaat
     1921 cgacgtcgca gtggcaggag gcagcgtacc aggcgcgcac ttgtaggcgg tgcccgcaac
     1981 cgtcaccgtc gagccggttc cgtaaccatg tgccatggcg ggctcggtgt cttgttccgt
     2041 aggctggtgc ccatctccct gagcgacttc ctcagggtgc aacaagaggt gcgtcgcccc
     2101 gagctcggcg gcctcgttga gtgcgtcgtt ctccgcggac tcttggttga cgcgcggctt
     2161 cgagtacgcg ccggtgagtc cgcctccgcg cccgatcacc acacccaggt tctcacagcc
     2221 cacgggctcc gcgtagagca tgacgaccgt cgtgccaccc tcgctgagcg cgtcggcttt
     2281 gcagccgagc aaatacgagc tggacaccaa tccgagcacg agaaaagcgc ggggaaagaa
     2341 acaagaaaat cgagacttca tgggcgagac tccttcgcaa acactgccta gccagggcgg
     2401 gaagtcaacc ctcttgcgcg ccagcaggat cgatcacgct cgctttcgag tccaccccac
     2461 tgtattctgc cgcgccggag aagctcatca tgtcggcaac cgtggaaact cgccaccgtt
     2521 cgctcgaaga cccggaaggg ttctggggtg aggtagcaga aggaatcgac tggatctctc
     2581 gttgggaccg agtcctcgat agctccaacc cgccattcta tcgctggttc cctggggccc
     2641 agatcaacac ttgttacaac gcgctcgatc gacacgtcga gaagcgcggt aatcaagctg
     2701 ccctgatcta cgacagcgcc gtgacccatc agcggcgtac gtatacgtat cggcagctcc
     2761 gcgacgacgt ggcccgtttc gctggcgcgc ttgcgtccca aggcgtcacc aagggcgacc
     2821 gtgtcctcct gtacatgccc atgattcccg aagccgtgat cgcaatgctc gcttgcgcgc
     2881 ggattggggc cgttcattca gtcgtattcg gtggtttcgc gccccaggag ctcgccaccc
     2941 gcatcgacga cgcagaaccc aagatcatcg tttcggcatc ctgcggcatc gagggcacgc
     3001 gagtcatcga atacaagccc ttgctcgatc aagcgatcga tctctcggag cacaaaccct
     3061 ccacttgcat cattcgacaa cgtgagagct gcgagtgcga cctcacggaa ggccgtgata
     3121 tttcctggca agatgcggta gcggcggcca agccccacga ctgcgtcccc gtgctcgcca
     3181 ccgacccgct gtacatcctc tatacttccg gaacgacggg cattcccaag ggcgtggttc
     3241 gggaccacgg tggccatgcc gtagctcttc aatggacgat gcgaaacgtg tacggcgtgg
     3301 agccgggcga cgtgtattgg gccgcgtcgg acgtgggctg ggtcgtcggg cattcgtaca
     3361 tcgtctacgg gcccttgctg aacgggaaca ccacgattct gtacgagggc aagcccgttg
     3421 gaactccgga cgcgggggca ttttggcggg tgatcgaaga ccatggcgtc aactgtctat
     3481 ttacggcgcc aaccgcattt cgagcgatca agaaggaaga cccaaacggt ctgctcctcg
     3541 ccgactacga cttgagcacg ttgcggtctt tgttcttggc tggtgaacgc tcggatcccg
     3601 acactctcaa atgggccgag gacaagctgg gtgtcccggt gatcgaccat tggtggcaga
     3661 cagaaactgg ctgggccatc tgtgcgaact gcctcggaat cgagcacctc ccggtgaagc
     3721 atggctctcc aacggtggcg gtccccggtt gggacgttca ggtggtggat gaagcttgcc
     3781 accagctcaa gccagggaag acgggcgccc tggtggtgaa gctgccgttg ccgccgggct
     3841 cgcttccgac gctttggaac gatgacgaac gcttcgtcaa gtcgtacatg accgagttcc
     3901 cgggttacta caagacggca gacgcaggat acatcgatga agatggttac gtgtacgtga
     3961 tgtcgcgcac ggacgacatc atcaacgtcg cggggcatcg tttgtcgacc ggtggcatgg
     4021 aagaggtggt cgcagcacac cccgatgttg cagagtgcgc ggtcatcgga gtcgccgatc
     4081 aactcaaggg cgagatcccg ctcggttttc tcgtgctcaa tgccggtgtc gaccgccctc
     4141 agcacgacat cgtctcagaa gtcatcgaga tggttcgcaa cgcgattggg ccggttgcgg
     4201 cgttcaaaac ggcaatcgtc gtgcaacgtc taccgaagac ccgttccggc aagatcctcc
     4261 gtgggaccat gaagaagatc gcagatcgca gtccttggaa gacgcccgcg accatcgatg
     4321 acccagtcat cctcgacgaa atccgggaac gtctgcaggc aatcgggtac gcctaaatga
     4381 acagagagca agagcgtgcc cttttgctct ttggcctcgc cgtcgtcctc attgcgacgc
     4441 cagtgcgagc gatttggctc caaacttggt ggtctccatt cgcgttctgg ctctcgatga
     4501 tcgccatcat cgcgctgctt catcgcatcg accatgcagc ttaatcactt cgttctcttc
     4561 tttctcgcgg ccgccctctt gggcctctac atcctgggat ccgaggtggt cgagcgctca
     4621 ccacgctggg ggcgcctcgg gcgaaaccgc ttcagcttcg cgctcgcgct cggcgtgtat
     4681 gcgacgacct ggacgctttt cggaagcgta ggattcgcag caacggaagg gtacgacttc
     4741 ctagccatct atttcggagt gacgttgtcg tgcatcgcga ttccgttcct ctgggccccg
     4801 gttgcggagc tcgtcgatcg ctatcgactg ggaagcattg cagacctgtt cgcgtttcgt
     4861 taccagagtc gaacgctcgg ggccgcggtt tcgatcttca tggttgcggg tttgcttccg
     4921 tatatcgcct tgcagcttcg tgcggtggcc gatggtgcgg cgatcctcgt cggacaggag
     4981 ccaagcgaag agctgggctc ggtgtacgca attctgctga gcgcattcgc tgtcgcgctc
     5041 ggtgtccggt acgccgacgg acgcgcgcat cggcccgggc tgatcgctac tttggcgttc
     5101 gaaagcatct tcaagctggt tgccctcttg gtcgtcggcg cagcaacctt gctgacggtg
     5161 tttggaggac tgcgtgggct caacgagtac cttcgtgacc gacctgagct cgtcgaaagc
     5221 atgacagacc ccgtgatggg ggactcttgg atcgcgctcg ttttcgtgac gttttgcgcg
     5281 gcgtttctct tgccgcgtca attcttcgtc gccttcgtgc agcggcccgg gcctcgcagc
     5341 ctcgagaccg cgcgttgggc cctgcccctc tacttgttcg cgatcaacct tcctgttccg
     5401 attctctatt gggcaggtcg ggatcttgca tcgacgggta tacggccgga cctctacgtt
     5461 ctcgtggcgg tgcagagctc gcccgctctt cagacactcg cgttcctcgg cgctgtctct
     5521 gcggccagcg cgatgatcct ggtatcgtcg atcgcgctga gcggaatggt cgccaactat
     5581 ctcgttcccc gattcggacc gtctccggag tccctgctga ctcgctgggc cgtatttcgc
     5641 cgttcgacga tcgtcggggt cgttctcgct ggcctgctga tccattttac gcttcctcgg
     5701 acgcgctcac tggtcgacat cggtctcgcg tcgttcgtcg cagtcaccca tctgattccc
     5761 ggcttgctgg gcgcgctgtt ctggcgaagg gcgacgcgat ccggcgtgct cgcaggtctg
     5821 atcgtgggcg cgacttcttg ggccgtgctg accgcgagtc ccttgtttgg tctggagctc
     5881 gacgcgtctc gcctctatgc gcttttcgga ttcaccgatc cttcagcgcg aggtccagcc
     5941 atctggctga gtctcaccgc tcacggcttc atgttcatcg gcgtatctct gctcaccgac
     6001 cgctctgcgg aagagcgcaa cgcagccgaa gcctgtcgtg agccacgtgt gaccaggctc
     6061 ctacagctcc ccgattcgcc cgaggcgctc caggagcgcc tgacctccta cgtcgggaag
     6121 gaaaccgcaa ttcgagagat ctacgaagcc gcggcggcgg ccgacgtcta ctggccgccc
     6181 cgagatcggc ccgagctcag tcgactgatc gccgagctcg aaggtcggct gagcgagcga
     6241 atcgggccgc ttgccgctcg cacctacgtt cagggagggc gccctgtacg cgccgacgcg
     6301 ctcgctgaac ggctccgtgc atttcgagag ctagactcgc tcgacgccga tgcatcacag
     6361 agaagctcgc cggcgcacgc cgcgcgtcgc tacctttcac agctcatgtc tgagctcccg
     6421 gttggggtct gtaccgtgga tgagcacgaa gacgtggtcg tctggaacgc gcaactggaa
     6481 cgaatgtcgg gtctcgcgga agacgagata tgcggcaatc ccatccacga cattccaagc
     6541 ccctggtggc ctctcttcga cgagctcgcc catcagccaa ccggctcggt gatcgagcgc
     6601 gaatacaacc tcggtgatcg gaacatcgat ctccgtgccc aaaccgcaac gattcctgga
     6661 ggaggggaag atgggcgcgt cttcgtcgtg gacgacatca cggcccttcg tcagctagag
     6721 cgacacgtag cccacaagga tcgcctctcc tcgatcggga cactggcctc gggcgtcgcg
     6781 cacgaggtcg gcaacccttt gaccggaatc ctgatggtgg cgaagaacct cgcgagcgac
     6841 cccggtgcag aggatgccgc ggaacgtcta ggcatcatca tccatgaagc gcagcgcatt
     6901 cacggaatcg tgcagagcct cctcacgttc agtcgacgcg acgagtcgcc gctcgaagtc
     6961 cagaccatct cggtcgccgc tgtagtgcgt gcggctgcgg agctcgccaa gctcgaaacc
     7021 caagggtgca ggatcgatct cgagctcgcc gtcgacgcca ccgccatggc gagctcggac
     7081 gggctgactc aggtgatggt caacctgctc aacaacgctc gtcaggcctg cccggccggg
     7141 gccaccgtct gcgtgcgaac gcgactcgac ccagaaaggg tctacatcga cgtcgacgac
     7201 gagggccctg ggattccaga agatctctcg gagcgcgtgt tcgagccgtt cttcacgacg
     7261 aagtcagatg ggcagggcac gggcctcggt ctctccgtct cgcgccagat cgtggagcgc
     7321 tttgggggga ccttgagcta cgaaccccgg gaaacagggg gcagcaggtt caccatcacc
     7381 ctcaaagcct tcgaaaacta gggcattgat ggatcgagcc ctcctttggt actaatcacc
     7441 ggtggcgaag atccttttgg tggaggatga gcgggtcatc cgtgcggaga tcaggcgcat
     7501 cctcacgcgc gatgggcacg aagtgaggga tgtggcgagc gtgccggcag cggagcagga
     7561 gcgtccggaa tcgttcgacc tcgtcatcac ggacgtgagg ctcacgggtg acacaggcga
     7621 cgtgttgatc gagcgcgtcg gacaggtccc cgtgatcgtg atgaccgcgt acggctcggt
     7681 gggcgcggcg gtcgacgcca tgaagcgggg cgcggcggac tacctttcga aaccgttcga
     7741 acccgaagcg ctgagcgccg cagtcgaccg tgttcttcgt caccgcgcct cgagcagcac
     7801 gcgcgacgtc gatccggcct tcgacccctt cgccatcgtc accacggaat cgagcccaat
     7861 gcaggcggcg ctgcgctgtg cgcggaaagt cgctccaacc gaggcgacgg tcttgatcct
     7921 gggcgagtcg ggcaccggga aagagctgat cgctcgctcc gtgcacaatc agagcgcccg
     7981 taccggtcga ttcgtccccg tcaactgtgc atcgattccg gagacgctgc tggagtcgga
     8041 gctgttcggg cacgagaaag gtgcatttac cggcgcgctc gcgcggcacg atggcctggt
     8101 acacgcagcc aacggaggaa cgctgttcct cgacgaaatc ggagagcttt cacatgcggc
     8161 gcaggcgcgc ttgctacgca tgcttcagca gcgtgaagtc cggcgcgtcg gcgcccagat
     8221 cgccgaagca gtcgacattc gcctgatcgc cgcgacccac cgaaatctcc caaccctcgt
     8281 ggaacgccaa gaatttcggg cagatctcta ctttcgactc aaggtcgtcg aaatccaact
     8341 gcctccgctc tgcgaacgtg gcgacgacat tctggccctc gccgaggtgt tcatcgaccg
     8401 cgccgctgag aagctcggcc gccggcccct cgagctttcg ccgggtgccg ccgcggcaat
     8461 cgaacggtac aaatggccgg gcaacgtacg cgagcttcag catgcggtgg agcgggccgt
     8521 gatccttcat gaatccggtc cgattaccga agatctgctc ggcttgcctc ggtcgacgcc
     8581 acccgagcac gtcgcgaacg gcgcaggcgg tgaagacccc tcactcgaag gttacctccg
     8641 gcgcttcgtg ctctcgcatc aggacgagct cagcgagacc gagctcgcca agcgcctcgg
     8701 cataagccgc aagacattgt gggagcgccg acaacgcatg ggcattccgc ggcgcgagtc
     8761 acggccgccc gagtagtcgc gagacgtttc gagagcgttt cattccgtag cgccttgcta
     8821 cgaatcgtaa cacttgctgc ggcgcgttag accgagatcc ggagtcgttt cgcgaagtcg
     8881 aattttggta cacgtcttgc gtagaagtcg gtagacctcg gtgtaaggtg taccgatgac
     8941 actcacacaa cccagcctcg attcaagaaa gcacattact agcctggagg agtacgaaac
     9001 cctctatcaa cgaagcctaa gcgatcgcga agagtactgg cttgcggagg gcaacgagct
     9061 ccactggttc tacgaaccgc acagcgtctt cgaagaacat ggtcccggtg aattcgtgtg
     9121 gttcgcgggc ggacgcctca acgcaacgat caattgcgtc gaccggcacg ccgacaagac
     9181 ccccgacaag acggccatca tcttcgtcaa ggatgagccg ggcgagtacg agcacatatc
     9241 gttcgcgcag ctcaagacgg cggtcggccg aatcgcaaac tccttgaaat caatgggtgt
     9301 ccagaagggc gaccgggtct gtatttacct tccaatggtg cccgagctcg catacacgat
     9361 gctcgcatgt gcccgaatcg gcgccgtgca ctcgatcgtg ttcggcgggt tcagcgcgga
     9421 ctcacttcga gatcgcatca tcgatgcgca agccaaggtc ctggtcaccg cgaacgaggg
     9481 tctccgtggc ggaaagcgaa tcccgctcaa gaagatctgt gacgacgcgg tcgaagggct
     9541 tgcagtcgtc gaaaaagtcc tcgtcgtcaa gcgtacggac accgaagtca acatggagca
     9601 cgaccgagac gtttggatgc acgaggcgat ggctgagcag cgcgtcacct gcccggcgga
     9661 atggatggct tcggaagatc cgttgttcgt tctctacacg tccggatcga ctgggaagcc
     9721 caaggggctg gtccatacga ccgccggcta cctcacattt gcagcttcta cgttcaaata
     9781 cgtcttcgat atccagcccg atgacatcca tttctgcgct gcagacatcg gttggatcac
     9841 ggggcacagc tacatcattt acggtccgct cgccaacggc atcaccaccg tcatgttcga
     9901 gtcggtaccc acatacccgg acgcaagcag gtattggaag gtcatcgatg acatcggcgc
     9961 gacgaccctc tacacctccc ctaccgcgct ccgggccctc atgcgtgagg gctcggagcc
    10021 cgtgcagggc tccagccgca agtcgcttcg cgtcttggga accgtcggtg aaccgatcaa
    10081 cccggaggtc tggcgctggt acagcgatgt ggtcggcaat ggaaagctgc cgatcgtcga
    10141 cacctggtgg caaacggaga ccggtggcat cttgatcagc ccgctcccca acgctactcc
    10201 cacgaagccc ggctcagcaa ccttcccctt cttcggtatc gaacccttgc tcgtggatca
    10261 agatgggaag gagatcgaag gcaatggggt cagcgggtac ctttgcatcc gacatccgtg
    10321 gccgggccaa gcgcgcacga tccacggcaa tcacaagcgt tttatagaga catacttctc
    10381 gcagtatccg ccattctact tcaccggtga cggctgccgt cgcgatgaag acgggtacta
    10441 ctggatcacc gggcgggtcg acgacgtaat caacgtctcg ggtcaccgca tgggcacggc
    10501 cgaggtcgaa agcgcgctcg tggcgcacca ggccgtcgca gaagcggctg tcgtaccatt
    10561 tccgcacgag atcaaaggcc agggcatctt tgccttcgtg gttccgatct cgggcgcaga
    10621 gtgggagccc catgagctcc agggcgcact gaaagaacag gtgcgtcaag tcatcggacc
    10681 gatcgcgacg ccggatgaaa ttcgcgtcgt gactgggctc ccaaagaccc gatccggaaa
    10741 gatcatgcga cgcatccttc gcaagattgc agccggtgaa ggcgatcaac tcggagatct
    10801 ctcgaccctc gccgacccga gcgtagtcga ggcgctgctg aagaaagttt gagagccgcc
    10861 tgcggcggag aaacggaaga tacaatggcg accgaatcag aagaattgcg tagctcgaaa
    10921 cacacgcaag acgttgccga tcgatactgg aagagaaatc ttcgcattct cttcgttcta
    10981 ttggcgatct ggtggggtgt ctcgttcgga gccgggatca tctgggccgg ttccctcgac
    11041 aacatcacca tcccgggcac cggattcaag cttgggttct ggtttgctca acagggttcc
    11101 atctacgtat tcgtcgtgct catcttcgtt tacacgcggt tgatgaacaa gctcgataaa
    11161 gagttcgacg tgcacgagga ctagtcgttc gagtcatcta aggagagaac aatggacgta
    11221 cagacttgga cattcatcat cgtgggcgga acattcgcgc tctacatcgg catcgcgatc
    11281 tggtctagag cgagcacgac cggcgacttc tacatcgccg gcagaggcgt ccatccgatc
    11341 gtcaatggga tggcgactgg agcggactgg atgagcgcgg cctcgttcat cagcatggcg
    11401 ggcctgatct cgttcatggg gtacgacgga gccgtctacc tgatggggtg gacgggcgga
    11461 tacgtcttgc tcgcgcttct cttggcgccc tatctccgca agttcggcaa gttcaccgtc
    11521 cccgacttcg tgggagaccg ctactattca aattcggccc gtatcgtcgc ggtgctttgc
    11581 gcggtcttcg tctccttcac ctacgttgcg ggtcagatgc ggggtgtcgg cgtcgtcttc
    11641 agccgcttct tggaggtcga catcaatgtc ggcgttctga tcggcatggc g
//
LOCUS       DASRZF010000417         2520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1009310, whole genome shotgun sequence.
ACCESSION   DASRZF010000417 DASRZF010000000
VERSION     DASRZF010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009310"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VFG22_18530"
     CDS             complement(<1..201)
                     /locus_tag="VFG22_18530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418300.1"
                     /translation="MQQLVSALLCLWLLFPSLARAEESEPAPSEESELAALRHSRSYQ
                     EGLTRVRESLERAYDMGAVRGAA"
     gene            complement(256..807)
                     /locus_tag="VFG22_18535"
     CDS             complement(256..807)
                     /locus_tag="VFG22_18535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418301.1"
                     /translation="MRRFNVLLSVALLSLALTFCGDSSGSGGAVDSDAALQAFMQTIA
                     LDFAQVLADVAPSVEAMTVKENGAADCPEGGAASWTESGLGGSGTLTLDACQMRGIAV
                     SGTLAGYLELGFEPSVNGTMMTGQITGSGRFDAELFVNSLNISAQIPITDFFTYWDIT
                     ATTTGGRSLCAWSGGSGCAPSPF"
     gene            complement(974..2452)
                     /locus_tag="VFG22_18540"
     CDS             complement(974..2452)
                     /locus_tag="VFG22_18540"
                     /inference="COORDINATES: protein motif:HMM:NF014503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418302.1"
                     /translation="MTFVQIPFMPSFATFWLGFRVPLVALCLLLVACGGSRRMLKETA
                     ALYEPDVARARSRASTIVFVPGIMGSQLYDTSDGQPVWGTFWSGGAWEETLEKMALPF
                     AIDQPVSALEDSIVPGGELLVAELALQSGALRARGYPGLFEGLVQELTEADTHHTPSA
                     LSIEDAVEGRDPILGFGYDWRREIASEVQRLHEVVVAASRERQLRTGNPRIDIIAHSM
                     GNLLVRWYLRYGTAPVPDDDSLPEVTWAGVQYVERVLLVAPPNMGEARALEVLLRGAE
                     QHPLLPVYPPAVVATFPSAYQLLPRLEDARVVYSDTGEPVDIYDVKVWEGFGWGPFAE
                     DQDDELSLLMPGVETRAERLALLRRHMDACLIHARRFHRALDVPEHPPPELRMHVFVG
                     DAHPTLAVLRVNRQTGAVDWADPEPGDGTLTRTSALGQSRTDLEAQPVFHPDSVHFNR
                     ADHLQMVGDRAFLDQALYLLLEETDPSAPATPAAPASLPEQK"
BASE COUNT          482 a          802 c          817 g          419 t
ORIGIN      
        1 tgccgcgccg cggacggcgc ccatgtcata agcacgctcg agggattcgc ggactcgcgt
       61 cagaccctct tggtaagaac ggctgtgacg aagcgcggcg agctcgctct cttcgctcgg
      121 ggcgggctcg gattcttcag cgcgggccag cgacgggaac agaagccaca agcacagaag
      181 ggcagatacg agttgctgca ttgcgcaacg ttggcccggt cgctcgggga gtgtcaaggg
      241 ggctctccgc agccactaga aaggcgaagg agcgcagcca gaaccgccgc tccacgcgca
      301 gagcgagcgg ccacccgtcg tcgtcgccgt gatgtcccag tacgtgaaga agtcggtgat
      361 tgggatctgc gccgagatgt tcagactatt gacgaacagc tcagcgtcga agcgtccgga
      421 gcccgtgatt tggccagtca tcatggtccc gttgaccgat ggctcaaacc cgagctcgag
      481 atagcccgcg agcgtcccgc tgaccgcgat ccctcgcatt tggcaagcat ccagagtgag
      541 ggtgccgctt ccgccgagcc cgctctccgt ccagctcgcg gccccgccct cggggcagtc
      601 cgcggcgcca ttctccttga ccgtcatcgc ctcgacgctg ggtgcgacgt cggcgaggac
      661 ttgcgcgaag tcgagcgcaa tcgtctgcat gaatgcttga agcgcagcgt cactgtcgac
      721 agccccgccg ctcccgctgc tgtcgccgca gaacgtcagg gccaaactca gcagagcaac
      781 gctgagaaga acgttgaacc gacgcataag atcctccctt actatggccg tcaggccgca
      841 catgaggact tcgcaaagac aatgccaagt gtgaaaccca agaattgcct gcagtccgca
      901 aaaccgcacg cgcaagctat gcgttttgag cccaccaggc gcagggaatg cggtccggga
      961 ttcgagcgat cttctatttc tgctctggta gggacgcagg cgcagctggg gtcgcaggcg
     1021 ctgaaggatc ggtttcctct agcaagaggt agagcgcctg gtcgagaaag gcgcggtcgc
     1081 cgaccatctg taggtgatcg gcccggttga agtggacgct gtctgggtgg aaaacaggct
     1141 gcgcttcgag atcggtgcga gactgaccga gcgcgctcgt tcgggtcagt gtgccgtcac
     1201 cgggctccgg atccgcccag tcgactgcgc cagtctgccg gttcacgcgc agcacggcga
     1261 gagtcgggtg cgcatcgccc acaaagacat gcatgcgcag ctcgggcggt ggatgctcag
     1321 gtacatcgag ggcgcgatgg aagcggcgcg cgtggatgag gcacgcatcc atgtgccttc
     1381 gcaggagcgc cagacgctcc gcgcgtgttt cgacgcccgg catcagtaag ctcagctcgt
     1441 cgtcctggtc ttcggcgaag ggtccccagc caaacccttc ccagaccttc acgtcgtaga
     1501 tatcgaccgg ctcgccggta tcggagtaca ccacgcgtgc gtcctcgagt cgcgggagca
     1561 gttggtaggc cgatggaaac gtggcaacga ccgcgggtgg atacaccgga agaagagggt
     1621 gctgttccgc tccgcgcaga agcacctcga gcgcgcgcgc ttcacccatg ttggggggcg
     1681 cgaccagcag cacccgctcg acgtattgga cgcccgccca agtgacctcc gggagcgagt
     1741 cgtcgtcggg cacgggcgcg gtcccgtagc ggaggtacca gcgcaccagc agattcccca
     1801 tcgaatgggc gatgatatcg attcggggat tgcccgttcg caattgtcgt tcgcgggacg
     1861 ccgcgaccac cacctcgtgg agccgctgca cctcggatgc gatttcgcgc ctccagtcgt
     1921 atccaaaacc gagaattggg tcgcgtccct ccaccgcgtc ctcgatgctg agggcgctgg
     1981 gggtgtgatg cgtgtccgcc tcggtcagct cctggacgag tccctcgaac aagccaggat
     2041 agccgcgcgc gcgtagtgcc ccgctttgaa gagcgagctc ggccacgagc agctctcccc
     2101 caggtacgat ggaatcttcg agcgcgctga ccggctgatc gatggcaaag ggcagggcca
     2161 tcttctcgag cgtctcctcc caggcaccac cgctccagaa cgtgccccag acgggttgcc
     2221 cgtcactcgt gtcatagagc tggcttccca tgatcccagg cacgaacaca atcgtcgagg
     2281 cgcgggaccg cgcgcgagcg acgtcgggct cgtagagcgc cgcggtttct ttgagcatgc
     2341 gcctgctccc tccacatgcc accaagagca ggcagagcgc gacgagcggc acgcgaaagc
     2401 caagccagaa ggttgcgaac gaaggcatga acggaatttg cacgaaggtc acgctccttc
     2461 aactgcccaa gccaaatcgg tcgcggtagc cggcgaacag ggggccatgt tgaaggagcg
//
LOCUS       DASRZF010000418         3374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_316791, whole genome shotgun sequence.
ACCESSION   DASRZF010000418 DASRZF010000000
VERSION     DASRZF010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316791"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VFG22_18545"
     CDS             <1..265
                     /locus_tag="VFG22_18545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418303.1"
                     /translation="RSPEVEASLRRVYERARHLIAESIRSADPEVAELDVLADQVLGL
                     VQAAALAFHLEPDRKHLRARLRAIGRLIETHVAYASLGLGTHP"
     gene            292..1131
                     /locus_tag="VFG22_18550"
     CDS             292..1131
                     /locus_tag="VFG22_18550"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HET6418304.1"
                     /translation="MMTQASTTPVQIEIRRNIRFNFAGSPAVHHRANPYLSHFWNALS
                     VMAPSTERILMRVARDVRAEIRDERLLKDLDALVAQEALHTREHRRLNARLSELGYDI
                     DGVNAELDQMFDDYVARADKPAAVALMIAGEHVIYAMSRALLEDPRMTEGMDAEVKGL
                     FLWHAAEEMEHQSVAHDVYVHLFGDGLSHRLVRARAFADAGRLLLGSLASVMKRLLSR
                     EPEGTATQRLEFLRFMAISPGYGRQFGRQALRYFQPGFAPWRDAGDLELIRKTLGSVS
                     PTA"
     gene            complement(1172..1372)
                     /locus_tag="VFG22_18555"
     CDS             complement(1172..1372)
                     /locus_tag="VFG22_18555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418305.1"
                     /translation="MHGTDRIEVPGATRLSPGSLRETLDGAMSTFQALALIIGGTASA
                     IYLLIMLQREIRSLELVLETGL"
     gene            1516..>3374
                     /locus_tag="VFG22_18560"
     CDS             1516..>3374
                     /locus_tag="VFG22_18560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqB family beta-propeller domain-containing
                     protein"
                     /protein_id="HET6418306.1"
                     /translation="MTSTPALFLALFVAVACGDTSSAETPTAAAGPDQTVSEGDAVQL
                     DGGGSSDPGGPTLSYAWTQVSGPPIALSDPAAIDPTFDAPLTPIQTTATVELRLTVTN
                     PDGASASDEVNIEVRSSDFAVFIADLVTPGFGELFKYDAETGAVAKLSEPSPFEFEPP
                     PVDAFTISPDGKWVAFRERVADFFNDFSDLYVVAADGSGLSRVSGGAGLSNGFVIDFA
                     WAPDSSRIAFVYAPSDTPGATTQLLTVIAGGDFSAQQSVSGPLAPGATVNDFEWSPDS
                     SGVAYRVAESLFTATPEGKERVEVSDPLVLQQITTFAWAPDGSRIAYLANELSTILPD
                     GTDRTPVNGILPIGRSVVGFQWSPDSTRLAYLADLDTDDVLELFTDSPRGGARAKVNA
                     ALPAGQGVQGFSWAPDGSRLGYYSGNRLYTGNPPAAPSSQVGVGLVGDLLLVGKPFEW
                     SPDSQWIAYGAEQDIADVLEFYVASSDGSNNQKVSGAMAAGGYAQFPIADQVWAPDST
                     RVLYRADETGDGALEVFSSVRDGSASDQLSVTPPGIVNAFPSAGLWSPDGSRVLYISD
                     IGSGDELWLQTPDGAEDPLPAYVAPEGSVGVYRSGRPPVAWARQPSPGEIVYAP"
BASE COUNT          594 a         1085 c         1071 g          624 t
ORIGIN      
        1 gcgctctccg gaggtcgaag cctcgctgcg gcgcgtgtac gagcgtgcgc ggcacctcat
       61 tgccgagagc atccgttcgg ccgaccctga agtcgccgag ctcgatgtgc tcgcagacca
      121 agtgctcggc ttggtgcaag ccgccgccct cgccttccac ctcgagccgg atcgcaagca
      181 cttgcgagca cgcctgcgtg cgattgggcg gctcatcgaa acccatgtcg cctacgccag
      241 cctcggcttg ggcacacacc cttagcttcc cgcagaaaag gaatgaacac catgatgacc
      301 caggcctcga ccacgcccgt ccagatcgaa atccgacgaa acatccggtt caacttcgcc
      361 ggtagcccgg cggtgcacca ccgcgccaac ccctacctga gccacttctg gaacgcgctc
      421 tcggtgatgg cgccgtccac cgagcgcatt ttgatgcgcg tcgcccgaga cgtgcgtgcc
      481 gagattcgcg acgagcgcct gctgaaagac ctcgacgcac tcgtcgcgca ggaggcgctg
      541 cacacgcgcg aacatcggcg gctcaatgcg cgtctctccg agctcggtta cgacatcgac
      601 ggtgtgaacg cggagctcga tcagatgttc gacgattacg tcgcgcgcgc ggacaagccg
      661 gccgccgtgg cgttgatgat tgcgggggag catgtgatct acgccatgag tcgcgctctg
      721 ctcgaagacc cgcgcatgac cgaggggatg gacgcagagg tgaagggtct tttcttgtgg
      781 cacgccgccg aggagatgga gcatcaatcg gtcgcgcatg atgtctacgt ccatctcttc
      841 ggagacggtc tttctcatcg cctcgtccga gcgcgcgcgt ttgccgatgc cggtcggctc
      901 cttcttggga gcctcgccag cgtcatgaag cggctgcttt cgagggagcc ggagggaacc
      961 gcgacgcagc gcctggagtt tttgcgcttc atggcgatct cgcctgggta cggtcgccag
     1021 ttcggaaggc aggcgctccg ctatttccag ccggggttcg cgccctggag agacgctggg
     1081 gacctcgagc tcattcgcaa gacgcttggc tccgtgtcgc cgaccgcctg agggctctcc
     1141 gccgttattc cacggtgact gggacgccct cctataaacc cgtctccagc acgagctcga
     1201 gcgaccgaat ctctctctgg agcatgatga ggaggtatat cgcagaggcc gttcctccga
     1261 taatcaaagc aagcgcctgg aaagtagaca tggcgccgtc gagtgtctca cgcagagaac
     1321 cgggagacaa tcgcgtggca ccggggactt cgatgcggtc cgtgccgtgc atggtgatgg
     1381 cgtcctttcg cggttcgctc ttgggatcga ggttcgcggg ctttgtgggc tcaaacgaac
     1441 ttgtatgctt tgatgtattc gcacttgttc atgcgaacga gatgtagagg agacgatgcg
     1501 atgcgacgaa gccgtttgac gtcaacacca gccctgttcc ttgcgctttt cgttgcggta
     1561 gcatgcggcg ataccagcag cgctgagaca ccgactgccg cggccggccc cgaccagacg
     1621 gtcagcgaag gcgatgcggt tcagctcgac ggcgggggaa gcagcgaccc gggaggcccc
     1681 accctcagtt acgcctggac tcaggtcagc ggccccccca ttgcgctcag cgaccctgcg
     1741 gcgatcgatc cgacgttcga tgcgccgctt actccaatcc aaacgactgc gacggtcgag
     1801 ctcagactca cggtcaccaa cccggatggc gccagcgcca gcgacgaggt gaatattgag
     1861 gtccgctctt cggacttcgc cgtcttcatc gcggacctcg tgacgcccgg cttcggcgag
     1921 ctcttcaaat acgacgcgga gaccggagcg gtcgccaagc tcagcgaacc atccccgttc
     1981 gagttcgaac cacctccggt tgacgcgttc acgatctcgc cggacggcaa gtgggtggct
     2041 tttcgggaaa gagtcgcgga ctttttcaac gatttcagcg atctctacgt cgtggccgcg
     2101 gacggttcgg gcttgagccg ggtgagcggc ggcgccggcc tcagcaacgg gttcgtaatc
     2161 gacttcgcgt gggcccccga cagttctcgc atcgcgttcg tttacgctcc aagcgacact
     2221 ccaggcgcca cgacgcagct cctcacagtg atcgcggggg gggatttcag tgctcaacag
     2281 agcgtgagcg ggcccctcgc gccgggtgcc acggtgaacg atttcgaatg gtcgcccgat
     2341 agcagtgggg tcgcgtatcg ggtggccgaa tctctcttca ccgcgacgcc cgagggcaag
     2401 gagcgagtcg aggtgagtga cccgctggtg cttcagcaaa tcacgacatt cgcctgggcg
     2461 cccgacggga gccgtatcgc ttacttggcc aacgagctca gcacgatcct tcccgacggt
     2521 accgatagaa ccccggtcaa cgggatcctg ccaatcggac gttcggtcgt ggggttccag
     2581 tggtctccgg acagcactcg tttggcgtac ctcgcagatc tggacacgga cgacgtcttg
     2641 gagttgttca ccgacagtcc gcgaggcggc gcccgcgcca aggtcaacgc agcgctgcca
     2701 gccgggcaag gcgttcaagg cttttcctgg gcgcccgatg gctcccgcct cggctactac
     2761 tcaggcaatc gactgtacac cggcaatcca ccggcagcgc cgagctcgca ggtgggtgtc
     2821 ggcctcgtgg gcgacttgct cttggtgggc aagcccttcg aatggtcacc ggacagccaa
     2881 tggatcgcgt atggggcaga gcaggacatc gctgacgtcc tcgagttcta cgtggcaagc
     2941 tccgacggga gcaacaacca aaaggtctcc ggcgcaatgg ctgccggcgg ctatgcacag
     3001 ttccccatcg cggatcaggt ctgggcgccg gacagtacgc gcgtgctcta tcgggccgac
     3061 gagactggcg atggggcgct cgaagtcttc agcagcgttc gcgacgggag tgcgagcgac
     3121 caactctcgg tcacgccacc gggaatcgtc aacgcattcc caagcgctgg cctctggtcg
     3181 cccgacggat ctcgcgttct ttacatctcc gacattgggt ccggcgatga gctctggctc
     3241 caaacaccgg acggagcgga ggatccgctg cccgcctatg tggcaccgga gggctccgtt
     3301 ggggtctacc ggagtggtcg tcctccggtt gcctgggcgc gtcaacccag ccccggcgag
     3361 atcgtctacg cacc
//
LOCUS       DASRZF010000419         6455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4447053, whole genome shotgun sequence.
ACCESSION   DASRZF010000419 DASRZF010000000
VERSION     DASRZF010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 6455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6455
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4447053"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VFG22_18565"
     CDS             complement(<1..609)
                     /locus_tag="VFG22_18565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418307.1"
                     /translation="MEWPIAIVIGSVAMGAVLGLVAPGRGRLLDAIRTFAIVSAVAVV
                     VGRLIPDAISGAGPIVLVAVAIAFVAPWLIERAAQEDPEHDHSSTMGLELSYWGLMLH
                     KLGDGLALGSFTGEAHVGHHHGDVLTAIAVHSVPVTALITLAFTSAHGRRSGIRRAVG
                     LAIVGSLGVVLAQQTPIGAWPGAEPWIEAAVGGLLLHVVTHDW"
     gene            complement(648..905)
                     /locus_tag="VFG22_18570"
     CDS             complement(648..905)
                     /locus_tag="VFG22_18570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418308.1"
                     /translation="MYRCKLCNRVSRPGERATKVVLERRPAEYPSRGKAQKGSTERRS
                     KFQDDPGGAGYEIAKECIACAPCAEAHLAKEAAQEAESLSI"
     gene            complement(1001..2005)
                     /gene="hemB"
                     /locus_tag="VFG22_18575"
     CDS             complement(1001..2005)
                     /gene="hemB"
                     /locus_tag="VFG22_18575"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HET6418309.1"
                     /translation="MAQNATPEFPGTRMRRNRRSDWVRRLNRESKLTPDDLIWPIFVQ
                     EGQGLRTAVPSMPGIERLSIDLAVKAVGEAKALGIPAVALFPVTPPELKTANGKEATN
                     PKNLVCRTVRSIKDAHDEIGVICDVALDPYTTHGQDGIVRDGEILNDETVKVLCEQAI
                     VQAHAGCDVIAPSDMMDGRIGTIRTALDAEALERVALLAYSAKYASAFYGPFRDAVGS
                     AQNLGSADKRSYQMDPGNTDEALREVALDVSEGADMLMVKPGMPYLDIIRRVKEAFGL
                     PTYAYQVSGEYAMLRGAAERDWLEWDKVILESMLCFKRAGSDGVLTYAAPEIAKLLGA
                     "
     gene            complement(2090..2974)
                     /locus_tag="VFG22_18580"
     CDS             complement(2090..2974)
                     /locus_tag="VFG22_18580"
                     /EC_number="3.8.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF002938.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018786 - haloalkane dehalogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HET6418310.1"
                     /translation="MTTVSAEDPHPRKRVAIEDSEMSYVDTGGSGPIAVFLHGNPTSS
                     YLWRNVIKEVSPVARCLAPDLIGMGDSGESSSGYRFVDHRRYLDAWFDEVVGAQPVFL
                     VIHDWGSGLGFHWAHRHAEQIKGIAYMEAIVRPLESWDDWAEPARKIFQAIRSPAGEE
                     IILEKNVFIERILPSSIMRKLSDAEMDRYRKRFAEPGESRRPTLTWPRQIPIGGEPED
                     VHDIAAAYAAWLQTSDSPPKLFIDADPGIVLIGPQREFARNLPNQESITVKGLHFIQE
                     DSPVEIGQAVASFVQKHS"
     gene            2992..3873
                     /locus_tag="VFG22_18585"
     CDS             2992..3873
                     /locus_tag="VFG22_18585"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HET6418311.1"
                     /translation="MSASRKWSAPESDYLVPFDGSFRVSSAPTGPPARVDEDAFEKAR
                     EERTAEIYELQRKLYADNRYSALFVFQAMDAGGKDGTIRSVFTGVNPAGFQVSSFKAP
                     SEQELDHDFLWRIAQRLPERGRIGVFNRSHYEEVLVVRVHPEFLERQRLPNMNLETLW
                     SERFGSIRDFEKHLAENGTVIVKFFLHVSKEEQKRRLLRRIDKPSRNWKFSAGDVEER
                     KRWPEYMASFEDALNQTSRPWAPWYAIPADDKPFMRLEVARIVQQTLASLGLDFPKVD
                     DAARLALADCRAALERQ"
     gene            3870..4676
                     /gene="thiD"
                     /locus_tag="VFG22_18590"
     CDS             3870..4676
                     /gene="thiD"
                     /locus_tag="VFG22_18590"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HET6418312.1"
                     /translation="MTETPPIVLTVAGSDPSGGAGLQADLKTLHQHGVYGAAVPTLIT
                     VQSSDGVAEVELLSADLVRAQLSQLLEDMEPAAAKTGALGSPAVAHVLGSVMRETRFP
                     WVVDPIWLPSRGKALSEGDVVEAYKKAVIPHAALITPNALEAGLLAGLSVRTLDDARV
                     AAERIAELGAAAVLVKGGHLEGGARGTDLLLVEGELSELSPAALVPGRFHGTGCALSA
                     AIAARLALGDDIPTAVKGAKTWLGAALAHAFAVGKGAQPVNHFWPIDAKP"
     gene            4733..>6455
                     /locus_tag="VFG22_18595"
     CDS             4733..>6455
                     /locus_tag="VFG22_18595"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing)"
                     /protein_id="HET6418313.1"
                     /translation="MPAGFRMARRRKKKGVPQIELPMDSIEDASLVVEAQRRYLNYAL
                     SVITSRALPDVRDGLKPVQRRILYTMYNELRLRSDVKHRKSAAIVGDVMGKFHPHGDT
                     AIYDALVRMAQSFTMRQPLVDGRGNFGSPDGDSAAAMRYTEARLQKLADELLLELAKK
                     TVEFRPNYDGRLFEPIVLPARFPNLLVNGSQGIAVGMATSIPPHNLAEVIQASVAMID
                     GDLTTSQLMKFVKGPDFPTGGELISSKSELTSAYETGQGSLKLRGEWKLEKPRSGNPA
                     IVIHSIPYSIERSTVVEKIAEVIIARKLPVLLDVRDESTDDCRIVCEMKKEADPQLIM
                     AYLYKNTPLQTNVQVNLTCLVPTANPQVAAPQRIGLKAILRHFLDFRMEVVTKRLEFE
                     LDQLLKRIHILEGFVLVFDALDEAIRIIRRSEGKQDAAVKLMKRFALDELQVDAILEL
                     KLYRLAKLQILEIRSELDQKLKEAQRVQKLLKSPAARWKLIRAELQELASLYADPRRT
                     RISAKLDEPEFDAEAFIIDEDAMVIVTEQGWVKRQQNLKDVASTRVRKGDRVLAVLAG
                     STKSTVAF"
BASE COUNT         1156 a         2039 c         2069 g         1191 t
ORIGIN      
        1 ccagtcatgc gtcactacat gcaataggag gccgcctacg gccgcttcga tccacggctc
       61 tgcacccggc caggctccga taggggtctg ctgcgcgagc acgacgccaa gcgagcccac
      121 gatcgccaag ccgactgcac gccggattcc gctacggcgg ccgtgcgcag atgtgaatgc
      181 cagggtgatc aacgcggtga ccggaacaga gtgaacggcg atggccgtga gcacgtctcc
      241 gtggtggtgg ccaacgtggg cctcgccggt gaacgagccc agtgcgaggc cgtcaccgag
      301 cttgtggagc atcagccccc aatagctgag ctcgagaccc atggtggagg agtgatcgtg
      361 ctccgggtcc tcctgtgcgg cccgctcgat cagccacgga gccacgaatg cgatggccac
      421 ggcgaccagg acgatgggac cggccccgct gatcgcgtcc gggatcaggc gtccgacgac
      481 cacggcgacc gcggagacga tcgcgaaggt tcggatggcg tcgagtagcc taccgcgccc
      541 aggggcgacg agtccgagca ccgctcccat cgcgacggat ccgatgacga tcgcaatcgg
      601 ccactccatc gggcgggctt accacactcg gaacgattct ggcgccgtta gatgctgagc
      661 gattcggctt cttgcgcggc ttccttggcc agatgcgcct cggcacaggg cgcgcacgca
      721 atgcattcct tggcgatctc gtagcctgcg ccgcccggat cgtcctggaa tttggagcgc
      781 cgttcggtgc tccccttctg tgctttgccg cggctcgggt actcggcggg acggcgctcg
      841 agcactacct tggtggctcg ctctccgggg cgcgacactc gattgcaaag cttacatcgg
      901 tacattcagg gactcctcgt tctgcgagcg gcgggtttgg cacatgggag gggtccgggc
      961 aagccaagcg gctcggggga gtccgcgccc gatcggccgc tcacgcgccc aaaagcttgg
     1021 cgatttcggg tgccgcgtac gtcagaacgc cgtcggagcc ggctcgcttg aagcagagca
     1081 tgctctcgag gatgaccttg tcccattcga gccagtcacg ctcggcagcg ccgcgtagca
     1141 tcgcgtactc accgctgacc tggtacgcgt acgttggaag cccaaacgct tctttcacac
     1201 ggcgaatgat gtcgaggtag ggcattcccg gcttgaccat gagcatgtcg gcgccttcgc
     1261 tcacgtcgag cgcgacctcg cgcagtgcct cgtctgtgtt gccagggtcc atttgatagc
     1321 tgcgcttgtc agcggagccg aggttctgag cggaacccac agcgtcacga aatgggccat
     1381 aaaacgcaga tgcgtatttg gcggagtacg cgagcagggc aacccgttcg agcgcctctg
     1441 catcgagcgc cgtccggatt gtgccgatgc ggccgtccat catgtcgctc ggcgcgatga
     1501 cgtcgcagcc ggcatgcgcc tgcacgatgg cctgttcgca aagcactttg accgtttcgt
     1561 cgttgaggat ctcgccgtcc cggacgatgc catcctgccc gtgcgtggtg tacgggtcga
     1621 gggcgacgtc gcagattacg ccgatctcgt cgtgcgcgtc tttgatgctc cggacggtgc
     1681 ggcagacgag gttcttgggg ttggtggctt ccttgccgtt ggccgtcttg agctcgggag
     1741 gggtcaccgg gaaaagcgcc acggccggga tgccaagagc cttcgcttca ccgacggctt
     1801 tcacggcgag gtcgatgctc aaccgttcga tgcccggcat cgaagggacc gcagtgcgga
     1861 gcccttgccc ttcctggacg aagataggcc agatcaagtc gtcgggtgtg agcttggatt
     1921 cacggttgag gcggcgtacc cagtcgctgc ggcgattccg ccgcatccgc gtgcctggga
     1981 actcgggcgt agcgttctga gccatggatg tcggcagcct agcatgcagc tatgctctgc
     2041 gcgtgagaat cggtcttcta gttctaacga cggcgctctg cggtagcgct caactgtgct
     2101 tctgaacgaa gctcgccacc gcttggccga tctcgacggg cgagtcctct tggatgaagt
     2161 gcagtccctt cacggtgatc gattcctgat tgggcaggtt ccgcgcgaat tcccgctgcg
     2221 gtccgatcaa gacgatgccc gggtccgcgt cgatgaagag cttgggcggc gagtcgctgg
     2281 tttgtagcca tgctgcatag gctgcggcga tgtcgtggac gtcctcgggc tctccaccga
     2341 ttgggatctg tcgaggccag gtgagcgtcg gtcggcgcga ctcgcccggt tctgcgaagc
     2401 gcttccggta gcgatccatc tccgcgtcgc tgagcttgcg catgatcgaa gacggcaaga
     2461 tgcgttcgat gaacacgttc ttttcgagga tgatctcttc cccggcaggc gaacgaatcg
     2521 cctgaaagat cttccgcgcc ggttcggccc agtcgtccca gctttcgagc gggcggacga
     2581 tcgcctccat gtacgcgatg cccttgatct gctcggcgtg ccggtgcgcc caatgaaatc
     2641 caagtcctga accccagtcg tgaatcacca ggaacacggg ctgggcccct acgacctcgt
     2701 cgaaccatgc gtccaggtac cgccggtgat cgacgaagcg atatccgctc gagctctcgc
     2761 ccgagtcgcc catgccgata aggtcgggcg ccaggcagcg ggcaacgggg gagacctcct
     2821 tgatcacgtt ccgccagagg tacgaggagg tcgggttgcc gtgcaggaac acggcgatcg
     2881 gtcccgaccc gcccgtgtcc acataggaca tttcgctgtc ttcgatggcg acgcgttttc
     2941 ttggatgggg gtcttccgcg gagactgtgg tcatggtgta tagtccgccc gatgagcgcg
     3001 agccgcaaat ggagtgcgcc cgagagcgac tacctggtgc ccttcgacgg gagctttcgc
     3061 gtgagctctg cgccgacggg ccctcccgct agggtcgacg aggatgcctt cgaaaaggca
     3121 cgcgaagagc gtacggccga gatctacgag ctccagcgca agctctacgc cgacaaccgc
     3181 tactccgccc tcttcgtgtt tcaggccatg gacgcaggcg ggaaggatgg caccattcgc
     3241 agcgtgttca cgggggtcaa tcctgcgggg ttccaggtgt cctcgttcaa agccccttcc
     3301 gaacaggagc tcgaccacga tttcctatgg cgcatcgctc agcgcctgcc ggagcgcggt
     3361 cgaatcggtg tcttcaatcg cagtcactac gaagaggtgc tcgtcgttcg cgtgcatccc
     3421 gagtttctcg aacggcaaag gctgccgaac atgaatctcg agaccctctg gagcgagcgt
     3481 ttcggctcga ttcgcgattt cgaaaagcat ctcgccgaaa acggcaccgt catcgtgaag
     3541 tttttcctcc acgtctcgaa ggaagagcag aagcgccgct tgctaagacg catcgacaaa
     3601 ccgagccgca actggaagtt cagcgccggc gacgtggagg agcgaaagcg ctggcccgag
     3661 tacatggctt ccttcgagga cgcgctgaac caaacttcac gtccctgggc gccttggtac
     3721 gcgatcccag cggacgacaa gcccttcatg cgcctcgaag tggcgcgcat cgttcagcag
     3781 actttggcga gcctcggtct agacttcccg aaggttgacg atgcggcgcg cttggctttg
     3841 gcagactgtc gcgcagcgct cgagcgtcaa tgaccgagac gccgccgatc gtgttgaccg
     3901 tcgcaggctc ggacccctcg ggtggcgctg ggctgcaggc ggatctcaaa acgctccacc
     3961 agcacggtgt ctacggagcg gcggtgccga cgctgatcac cgtccaaagc agcgatggcg
     4021 tcgcggaggt cgagcttctc tcggcggacc tggtccgagc tcagctcagt cagctcctcg
     4081 aggacatgga gcccgccgcg gccaagaccg gcgcactcgg gtctcctgcg gttgcgcacg
     4141 tcctcggctc ggtgatgaga gagacgcgct ttccctgggt ggtggatccg atctggttac
     4201 cgtctcgggg caaggccctg tccgagggcg acgtcgtaga ggcctacaag aaggcggtca
     4261 ttcctcacgc ggcgctgatc actcccaatg cgctcgaggc ggggctcttg gcgggacttt
     4321 ccgtgcggac gctcgacgat gcccgagtcg cggcggagcg aattgccgag ctcggtgcgg
     4381 ccgccgttct cgtgaagggc ggccatctgg agggcggggc tcggggcacg gacctgcttc
     4441 tcgtcgaggg ggagctcagc gagctcagcc cagcggcgct cgtgcccgga cgattccacg
     4501 ggaccggctg cgcgctctcg gcagcgatcg cggcgcgcct tgccctcgga gacgatattc
     4561 cgacggcggt aaaaggcgca aagacctggc tcggcgcagc gctcgcccat gccttcgccg
     4621 tcggcaaggg cgcccagcct gtgaatcatt tctggcctat cgacgccaaa ccttgatcgg
     4681 attcgaagtt tcgagatcgc aatagttgcc ccgtcttttg cgaggctgct acatgccagc
     4741 aggttttcgg atggcgcggc gtcggaagaa gaagggggtt ccccagatcg agctgcccat
     4801 ggacagcatc gaggacgctt cgctcgtcgt cgaggcgcag cgcaggtatc tcaactacgc
     4861 gctgagcgtc attacctctc gcgcgctgcc agacgtccga gacgggctca agcctgtgca
     4921 gcggcgcatc ctctacacga tgtacaacga gctccggctc cgcagcgacg tcaagcatcg
     4981 caagtcggcg gccatcgtcg gcgacgtgat gggcaagttc caccctcacg gggataccgc
     5041 gatctacgat gcgctcgtcc gcatggcgca gtccttcacg atgcgccagc ccctggtcga
     5101 cggccgaggg aacttcggct cacccgatgg cgattcggcg gcggccatgc ggtacaccga
     5161 agcgcgcctt caaaagcttg cggatgagct tctgctggag ctcgcaaaga agaccgtgga
     5221 gtttcgcccg aactacgatg ggcgcctgtt cgaaccgatc gtactcccgg cccgctttcc
     5281 aaacctcctc gtaaatggct cgcagggcat tgcggttggc atggcaacct cgattccgcc
     5341 tcacaatttg gccgaggtga ttcaggcctc cgtggcgatg atcgatggcg acctcacgac
     5401 ctctcagctg atgaagttcg tgaagggtcc ggacttcccc accggcggag agctgatcag
     5461 cagcaagagc gagctgacct cggcctacga gaccggccag ggctcgctga agctccgagg
     5521 tgagtggaag ctcgagaagc cccgaagcgg taatcctgcg atcgtgattc actcgatccc
     5581 ctactcgatc gagcgcagca ccgtcgtcga aaagatcgcg gaggtgatca tcgcgaggaa
     5641 gctaccggtc ctgctcgatg tgcgcgacga gagcacggac gattgccgga tcgtctgcga
     5701 gatgaagaag gaggccgacc ctcagctcat catggcgtac ctctacaaga acacgccgct
     5761 tcagacgaac gtgcaggtca acctcacctg cctcgtaccg accgccaatc cgcaagtggc
     5821 ggcgccccag cgcatcggcc tcaaggccat ccttcggcac ttcctcgact tccgcatgga
     5881 agtggtcacc aagcggctcg agttcgagct cgaccagctc ctcaagcgaa tccacatcct
     5941 agagggcttc gttctcgtgt tcgacgcgct cgacgaggcg attcggatca tccggcgctc
     6001 cgagggaaag caggacgccg cggtcaagct gatgaagcgt ttcgcgctag acgagctcca
     6061 ggtagacgcc atcctagagc tcaagctcta ccgcctcgcg aaactacaga ttctcgagat
     6121 tcggtccgag ctcgaccaga aactcaagga agcccagcgc gtgcagaagc ttctcaagag
     6181 cccggcggcg cgctggaagc tgatccgtgc cgagcttcag gagctcgcct ctctctatgc
     6241 ggaccctcgc cgtacgcgga tcagcgccaa gctcgacgag cccgagttcg acgccgaggc
     6301 cttcatcatc gacgaggacg cgatggtcat cgtcaccgaa cagggctggg tcaagcgtca
     6361 acagaacctg aaggacgtgg cctcgacgcg agtgcgaaag ggcgaccgag tgctcgccgt
     6421 gctcgcgggc tcgacgaaga gcaccgtggc ttttt
//
LOCUS       DASRZF010000420         6192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3418013, whole genome shotgun sequence.
ACCESSION   DASRZF010000420 DASRZF010000000
VERSION     DASRZF010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 6192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6192
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3418013"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VFG22_18600"
     CDS             complement(<1..182)
                     /locus_tag="VFG22_18600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HET6418314.1"
                     /translation="MARRRLGRGLDGLLPAAPPAAERSAKNVAAIEDLHPGRMQPRGR
                     IEAEGLAELAASIREHG"
     gene            complement(182..952)
                     /locus_tag="VFG22_18605"
     CDS             complement(182..952)
                     /locus_tag="VFG22_18605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HET6418315.1"
                     /translation="MSRVVAIANQKGGVGKTTTAVNLGASLAVAEQRVLLVDLDPQGN
                     ASTGVGISPGDEDLEGSYELLLGEVETADVIYETDMPYMHVIAATQDLVGAERDLLRA
                     DDSATRLREALADVRDDYDFIVIDCPPSLGMLTINALTAADMVIVPLQCEYYALEGLS
                     QLVGTIERVRASVNEHLEIHGVLLTMFDSRNNLANEVADEVRRHFHVYETIIPRNVRL
                     AEAPSHGKPVILYDASSRGSYTYLNLARELLDDLEVAA"
     gene            1104..1283
                     /locus_tag="VFG22_18610"
     CDS             1104..1283
                     /locus_tag="VFG22_18610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418316.1"
                     /translation="MRPRGPKNYQSLAEFEREELRRHHKAGWSLDDLYSEATFEPGED
                     DSLVSDERQELDFDF"
     gene            complement(1309..1542)
                     /locus_tag="VFG22_18615"
     CDS             complement(1309..1542)
                     /locus_tag="VFG22_18615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418317.1"
                     /translation="MSDTYNNVPMSDSMAARTEKEDAFWTGPRLIGLSFATLAVAFAL
                     LWSMAQGIIGPTQHDAEYGHEQHLLQPGGGPLD"
     gene            1683..2540
                     /gene="map"
                     /locus_tag="VFG22_18620"
     CDS             1683..2540
                     /gene="map"
                     /locus_tag="VFG22_18620"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HET6418318.1"
                     /translation="MSAIELMTPRMLQDMRRSCQLAADCLTAVGEMIRPGIVTNEIDL
                     FVHEYIVSRDAYPSPLDYKGFPKSVCTSVNDCVCHGIPGDQRLEDGDIVNVDVTTYLP
                     KEHGYHGDTSVTFYVGEPSPEAILVVETARRCLELGIAEVKHGNRIGDIGAAIQEYAE
                     SKGCSVVRDYVGHGVGREFHMPPQIPHFGRRGKDKRMKAGMVFTIEPMINIGGYQTEV
                     MDDDWTVLTLDRSLSAQFEHTVLVTRDGVEVLTARKAIVRHSEDKPWSDVGPLGSPAA
                     WNARREKTG"
     gene            complement(2552..2905)
                     /locus_tag="VFG22_18625"
     CDS             complement(2552..2905)
                     /locus_tag="VFG22_18625"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HET6418319.1"
                     /translation="MEFWVCREDELHPGQVRTKQLGHDAKGLPISALLLRDESGAVVA
                     YRNLCRHLPVPLDGGTGDFLSEDGSHLVCGTHGATYRLRDGHCIEGPCEGLALERLKV
                     RSVDGDLYVCPPEAG"
     gene            complement(2911..3588)
                     /locus_tag="VFG22_18630"
     CDS             complement(2911..3588)
                     /locus_tag="VFG22_18630"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET6418320.1"
                     /translation="MSGQTFIVGDVHGCREELEDLLEASGWEDEDQLVLVGDLVAKGP
                     DSRGVIRLVRKLGARAVRGNHDEHCLKWWRAERAGEPVPALKPAHQQVVDTLEDEDWE
                     WLSGLPLWIDLPEHDALVVHAGLVPELPLEEQTPHDLMNMRSILDDGSASRSYEEGTP
                     WAELWPGPRLVVFGHDAVRGLQKRSHALGIDTGCVYGGWLTGLWLPARDLVSVPARDT
                     YALPGMD"
     gene            3682..4152
                     /locus_tag="VFG22_18635"
     CDS             3682..4152
                     /locus_tag="VFG22_18635"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HET6418321.1"
                     /translation="MLKEGRKAPAFNLPASTGGKLALKDLAGKYVILYFYPRDNTPGC
                     TTEANDFNKALSKIKARDAVVVGVSKDSIESHCKFADKYKLKFPLLSDPDGKMLEKYG
                     AWGEKNMYGKKSMGIIRSTVLIGPDGKVIKHFPKVNVKGHVDAVIDALNQARSN"
     gene            4188..5132
                     /locus_tag="VFG22_18640"
     CDS             4188..5132
                     /locus_tag="VFG22_18640"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HET6418322.1"
                     /translation="MSEPSPMPAPEAPEALDPNFRIELPNFEGPLDLLLHLIRKHELE
                     ILDLPISFITDKYLEYLGLMSELNLDIASEYLVMAATLAHIKSKMLLPRPPVDQDDEE
                     LDQLDPRAELIRRLLEYQKYKLVAADLAERAIAGRDVFSRGSPPPTADGLPPLADVSV
                     FKLLDALKKIAQRINASISLEVDAERMSIQDRIGDLVDLLRERRRCRFDELFEGVSTS
                     YELVVTFLALLEMAKMRLASIYQTDHEEPIYLEYTLLDATGEPMVPADVQQPTSDLVD
                     ADFEFGERVETVDDRRESVVVPAGTSAGYAWNEDEQER"
     gene            5129..5926
                     /gene="scpB"
                     /locus_tag="VFG22_18645"
     CDS             5129..5926
                     /gene="scpB"
                     /locus_tag="VFG22_18645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HET6418323.1"
                     /translation="MTQPNVQSDNGAADGGLEARLAPGADSLKNVVESLIFVSDSPVS
                     AKKLARAARATIAEVQPLLDELVADYQHRGVHLYFVAGGYQFRSARESADFVKTLVAP
                     KPIRLTRAQLETLAIVAYRQPLTRPEVDDIRGVDSGSSLKMLADRGLVKILGRKDEPG
                     RPLVYGTTPQFLEFFGLSALKDLPTLQEFSDLTDEHKALFEQKTGESIDIAAAELAAA
                     EALEQAEEELLAEEEAAIAAVEAATAAEDDDEDDEDDEDDEDDEDEA"
     gene            5923..>6192
                     /locus_tag="VFG22_18650"
     CDS             5923..>6192
                     /locus_tag="VFG22_18650"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HET6418324.1"
                     /translation="MTFQDLILALERFWAERGCLIVQPYNSEVGAGTFNPHTFLRAIG
                     PEPWNVAYVEPSRRPTDGRYGENPNRLQQFHQYQVILKPSPLDIQE"
BASE COUNT         1272 a         1970 c         1848 g         1102 t
ORIGIN      
        1 ccgtgctcgc gaatcgacgc cgcaagctcg gccaggccct ccgcctcgat gcgcccacgt
       61 ggctgcatgc gccccgggtg caggtcttcg atggccgcga cgttctttgc ggagcgttcc
      121 gcggcgggag gcgccgcagg aagcaatccg tccaggccac gccccaaacg tctgcgcgcc
      181 actatgccgc tacctccaga tcatcgagca actcgcgcgc caagttcaga tacgtgtacg
      241 aaccacgcga ggaggcgtcg taaagtatca cgggctttcc gtgtgaaggc gcctccgcaa
      301 gccgcacatt tctcggaatg atcgtctcat agacgtgaaa gtggcgacga acttcgtccg
      361 caacttcgtt ggccagattg ttccgcgaat cgaacatcgt gagaagcacg ccgtggatct
      421 cgagatgttc gttgaccgac gcccggacac gctcgatcgt gcctacgagc tgcgacaagc
      481 cctcgagcgc gtagtattcg cactgcaaag gaacgatcac catgtccgcc gcggtgagcg
      541 cgttgatcgt gagcatcccg agcgaaggcg ggcagtcgat gacgatgaag tcgtagtcgt
      601 cgcggacgtc agcgagcgct tcgcgcaatc gcgtcgcgct gtcgtcggct cggagcaagt
      661 cgcgctcggc acccaccaag tcctgcgtcg ccgcgatgac atgcatgtac ggcatgtccg
      721 tttcgtaaat cacgtcggcg gtttcgacct cgccgaggag gagctcgtag gagccttcga
      781 ggtcttcatc accggggctg atgccgaccc ctgtcgacgc gttgccctgc ggatcgagat
      841 ccaccaacag aacgcgctgc tccgcaacgg caagactggc gcccaggttg accgccgtgg
      901 tcgtcttccc aacaccgccc ttctgattgg cgatcgccac cacccgactc atggactgcg
      961 tctaccagtg ccagtgcgcg gtttcaatct cgtctgtttt tttgccatct cgatccgcct
     1021 gggtagggtg tgctgcagtg taggacaaag tagtcactcg ccataccgag ccttggtacg
     1081 ggagctggca aaagggaggg gacatgcgcc ctagaggacc gaagaactat cagtcgctgg
     1141 ccgagttcga gcgcgaggaa ttgcgccgcc accacaaggc tggctggtct ctcgacgatc
     1201 tctactccga agctaccttc gagccgggcg aggacgattc cctcgtgagc gacgagcgcc
     1261 aggagctcga tttcgacttc taagcgcgaa atcgcttaga agcttcaatc agtcgagcgg
     1321 accgccgccc ggctgaagca ggtgctgctc gtggccgtat tcagcatcgt gttgggtggg
     1381 cccaatgatt ccctgagcca tggaccagag cagggcgaag gcgacagcca gagtcgcgaa
     1441 gctgagaccg atgagcctag gcccggtcca aaacgcgtct tccttctcgg tgcgcgcggc
     1501 catcgagtcg ctcatcggaa cgttgttgta cgtgtctgac attgaaaagc cctagaaatc
     1561 ctctcggaac ggctacttag tgccatcccg cggacaggtc aaacctgctt cgaacggccc
     1621 tttcgctcga caatttgcga ccccgtccta gaagcactag cacgcggcct ttgaggaatc
     1681 ctttgtctgc tatcgagctc atgacacccc gcatgctcca ggacatgcgg cgatcgtgcc
     1741 aactggctgc cgactgcctg accgcggtcg gggagatgat ccgccccggc atcgtgacga
     1801 atgagatcga cctcttcgtc cacgagtaca tcgtctcacg ggacgcttac ccctctccgc
     1861 tcgactacaa gggatttccg aagagcgtct gcaccagcgt caacgactgc gtctgtcacg
     1921 gcattccagg ggaccagcgc ctcgaagatg gggacatcgt gaacgtcgac gtgaccacgt
     1981 atctcccgaa agagcacggc tatcacgggg atacgagcgt gacgttctac gtgggcgagc
     2041 cgagcccgga agcgatcctc gtcgtggaga cggcccgtcg ctgcctcgag ctcgggattg
     2101 cagaagtgaa gcacggaaat cgcatcggtg atatcggtgc cgccattcag gaatacgcgg
     2161 aaagcaaggg ttgctcggtc gttcgcgact acgtcgggca cggggttggg cgagagttcc
     2221 acatgccgcc ccagattccg cacttcggca ggcgtgggaa agacaaacgt atgaaggccg
     2281 ggatggtctt cacgatcgag cccatgatca acatcggggg gtatcagacc gaggtcatgg
     2341 acgacgactg gacggtactc accctggacc gttccttgtc ggctcaattc gagcacacgg
     2401 tcctagtgac ccgcgatggg gtcgaggtgc tgactgcgcg caaagcgatc gttcggcaca
     2461 gtgaagacaa gccatggtcg gatgtcggcc cgcttgggtc gccggcggct tggaatgccc
     2521 ggcgagaaaa aacgggctga cgaacgcggt ctcagcctgc ctccggcgga caaacgtaca
     2581 aatctccatc gacgctccgc accttcaatc tctcgagcgc gagtccttcg cagggtcctt
     2641 cgatgcagtg gccatcccgc aatcgatacg tcgctccatg cgtcccgcag accaaatgcg
     2701 aaccgtcctc gctaagaaaa tcaccggtgc cgccatcgag aggaaccggc aggtgccggc
     2761 acagatttcg gtacgcaacg acggcgccac tttcgtcgcg aagcagcaac gctgaaatgg
     2821 gaagcccttt ggcatcgtga ccgagctgct tcgtgcggac ctggcccgga tgaagctcgt
     2881 cttcgcggca gacccagaac tccaagacgc tcaatccatc cctggcaggg cgtaggtgtc
     2941 acgcgccggc accgacacga gatcgcgcgc cggcaaccag agccccgtca accagcctcc
     3001 atacacgcaa cccgtatcga tcccgagcgc atgcgatctc ttttggagcc cgcgaacggc
     3061 gtcgtgcccg aagaccacca ggcgcggccc aggccagagc tcggcccagg gcgtcccctc
     3121 ttcgtaactc cgcgacgcgc ttccatcgtc gagaatgctg cgcatgttca tcagatcgtg
     3181 gggtgtctgc tcttccagcg gaagctccgg aaccagccca gcgtggacca cgagcgcatc
     3241 gtgctcgggc agatcgatcc ataggggcag gcccgaaagc cattcccaat cctcatcttc
     3301 cagcgtgtcg accacctgct ggtgggcggg cttcagggcc ggcactggct cgccggcacg
     3361 ctcggcccgc caccatttca agcagtgctc gtcgtgattc ccgcgaacgg ccctggcgcc
     3421 gagcttcctc acgagtcgaa taacccctcg cgaatccgga cccttggcga ccaaatctcc
     3481 gaccagaacc agctgatctt cgtcttccca acccgaggcc tccagaaggt cttccagctc
     3541 ctcccggcag ccgtggacat cgcccacgat gaaagtctgg ccgctcatgt cccgatcttg
     3601 ggcgcgtctc tggaggattc caagttcgtg agccccgtgg agcgttgaca gaaggcgcaa
     3661 acccgcaata cacgcgtctc catgctgaaa gaaggccgca aggctcccgc gttcaatcta
     3721 cccgcatcca ccggtggcaa gctggccctg aaagatctcg ctggcaagta cgtcatcctc
     3781 tacttctacc cacgcgacaa cacgcccggc tgcaccacgg aagccaatga cttcaacaag
     3841 gccctgagca agatcaaggc gcgcgatgct gtggtcgtcg gagtcagcaa ggactcgatc
     3901 gagtcgcatt gtaagttcgc tgacaaatac aagctgaagt tcccgttgct cagcgacccg
     3961 gacggcaaga tgctcgagaa gtacggcgct tggggcgaaa agaacatgta cggtaagaag
     4021 tcgatgggga tcatccgttc gacggtgctc atcgggcctg atgggaaggt catcaaacac
     4081 ttcccgaagg tcaatgtgaa gggccacgtc gacgcggtga tcgacgccct caatcaagcc
     4141 cgtagcaatt gacactaggg agcgtcacag cgcatggttc cacagcgatg agcgagccca
     4201 gccccatgcc ggcgcccgag gccccggagg ctctggaccc caacttccgg atcgagctgc
     4261 ccaacttcga agggccgttg gatctgctct tgcacttgat ccgcaaacac gagctcgaga
     4321 tcctcgatct gccgatctcc ttcattaccg ataagtacct ggaatacctg ggattgatga
     4381 gcgagctcaa cctcgacatc gcctcggaat acctcgtgat ggcggcaacg ctggcgcaca
     4441 tcaagagcaa gatgctcctt ccgcggcctc ccgtggatca ggacgacgaa gagctcgacc
     4501 agctcgatcc tcgcgccgag ctcatccgcc gcctgctcga ataccagaag tacaagttgg
     4561 tcgccgcaga cttggcggaa cgcgcgatcg cggggcgaga tgtgttttcc cgcggcagcc
     4621 cgccgccgac cgcagatgga ttgccgccgc tcgcggacgt gagcgtcttc aagctactgg
     4681 acgcgctcaa gaagattgca caacgcatca atgcgtcgat ttcgctcgag gtcgacgcgg
     4741 agcggatgtc gattcaagac cgaatcggtg acctggtgga cttgctccgt gagcgtcgcc
     4801 gatgccgctt cgacgagctc ttcgagggag tgagcacgtc gtacgagctc gtcgtcacgt
     4861 tcttggcgct tctcgagatg gcgaaaatgc gtctagcgag tatctaccag accgaccacg
     4921 aggagccgat ctacttggaa tacactctgc tcgatgccac gggcgagccc atggtgcccg
     4981 cagacgtgca acagcccacc agcgatctcg tggatgcaga cttcgaattc ggtgagcgag
     5041 tcgaaacggt cgacgaccga agagaatccg tggtcgtccc ggcgggcact tcagccgggt
     5101 atgcatggaa cgaggatgag caagagagat gacccaaccc aacgtgcaat cggacaatgg
     5161 cgccgccgac ggcgggctcg aagcgagact cgcgcccggt gcggattcgc tcaagaacgt
     5221 cgtcgagagc ttgatcttcg tttctgactc cccggtgagc gcgaagaagc tcgcccgggc
     5281 ggcgcgggca acgatcgccg aggtccagcc tttgctcgac gagctggtcg cggactacca
     5341 gcaccgcggt gtgcatcttt atttcgtggc cggcggctat cagtttcgct cggcgcgcga
     5401 aagcgcggac ttcgtcaaga cgctggtcgc gccaaagccg attcgcctca cgcgggccca
     5461 gctcgaaacg cttgcgatcg tcgcgtaccg acagcctctc acccgacccg aggtggatga
     5521 catacgtggg gtcgactcgg gctcgtcgct caaaatgctg gctgaccgcg gtttggtgaa
     5581 gatcctcgga cgcaaggacg agccgggaag gcctttggtt tacgggacga caccccagtt
     5641 cctcgagttc ttcggcctca gcgctctcaa agaccttccg acgctgcaag agttcagcga
     5701 tctcacggac gagcacaagg cgctgttcga acagaagact ggagagtcga tcgacatcgc
     5761 cgctgccgag ctcgccgccg cagaagccct ggagcaagcc gaagaagagc ttctcgccga
     5821 agaggaggcc gccatcgccg cggtggaagc tgcgacggcc gctgaggacg acgacgaaga
     5881 cgacgaagac gacgaggacg acgaggacga cgaggacgag gcatgacctt ccaagacctg
     5941 attctcgccc tcgagcgatt ctgggccgag cgaggttgcc tcatcgtcca gccctacaac
     6001 tccgaggtcg gcgccggcac gttcaatccg cacacctttt tgagggcgat cggacccgag
     6061 ccctggaacg tcgcctatgt cgagcccagc cgcagaccga cggacggccg ctatggagag
     6121 aaccccaacc ggcttcagca gttccatcag taccaagtga ttctcaagcc ctcgccgctc
     6181 gacatccagg ag
//
LOCUS       DASRZF010000421         2167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1700509, whole genome shotgun sequence.
ACCESSION   DASRZF010000421 DASRZF010000000
VERSION     DASRZF010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1700509"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VFG22_18655"
     CDS             complement(<1..765)
                     /locus_tag="VFG22_18655"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HET6418325.1"
                     /translation="MKKLILVLIAMSLAALGCDSGSESPGGAGGDGGAGEMGGDGGAG
                     GAEPKCLTPQAMLRCDKVLNIAHRGGRRIRPEHTLLAYDQALADGADILELDVHETLD
                     GVLVVMHDDTVDRTTDCTGPIKEKTLAELRVCDAGYNFTRDDGATYPYRGTGLVVPTM
                     EEVFDRYPDTPFVIEVKQSDPPIVDHFVEVIREHGVEDKMTGSAFSDAVLAQLRAAAP
                     EIPTNTGVNETLQFWGFSFDELDPEYVPPAEFLQVPT"
     gene            858..>2167
                     /locus_tag="VFG22_18660"
     CDS             858..>2167
                     /locus_tag="VFG22_18660"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET6418326.1"
                     /translation="MSTRSAFTFCRICEATCGLKATIEGNRVTAVEPDPEHVVSKGYS
                     CIKGLRYHEIHHSPDRLRVPLKRIGDVFEEISWEQALDEIGAKVKQLVKEHGGDSISA
                     YLGNPISFSLLPPILTSGFLQGLGSRNLFQTGSQDCNNKFAAAQRVYGFPFIQPFADV
                     DRTQCLIIIGSNPAVSRSSFMQLPDPVRRLRAIEGRGGRVFLVNPRRTETAKQLGTQV
                     FIRPDTDVYFLLSFLHEVIQRDGIDHERVGRYMRGLNALRRVAEPWSPERSAEVTGIP
                     AETLRAMVDAYLEADGAALFLSTGVNQGSHGTLAFWIQEAINAITGNLDRLGGTLVGH
                     GFIKDFPKHARKGGHTMRKDLSRIGKLPSVADTFPAGLMADEILTPGEGQVRALFCVS
                     GNPLLTVPNANGRLEEALKALELLVVIDIFRNETANHAHYILPGT"
BASE COUNT          389 a          688 c          675 g          415 t
ORIGIN      
        1 cgtcggcacc tgaaggaact cggccggggg gacgtactcc ggatcgagct cgtcaaacga
       61 gaagccccag aactgaaggg tctcgttgac gccggtgttg gtggggattt cgggcgccgc
      121 ggcgcgcaac tgggcgagga ccgcatcgga gaacgccgag ccggtcatct tgtcttcgac
      181 gccgtgctca cggatcacct cgacgaagtg gtcgacgatc ggcgggtcgg attgcttgac
      241 ctcgattacg aatggggtgt ccgggtaacg gtcgaagacc tcttccatcg ttgggacgac
      301 cagacccgtg ccgcggtatg ggtaagtggc tccgtcgtct ctggtgaagt tgtagccggc
      361 atcgcacacg cgaagctcgg cgagtgtctt ctccttgatc gggccggtgc agtccgtcgt
      421 gcggtcgacc gtgtcgtcgt gcatcacgac gagcacgcca tcgagggtct cgtgtacgtc
      481 gagctcgaga atgtcggcgc cgtccgcgag cgcctggtcg taggcgagca gcgtgtgctc
      541 gggtcgaatg cgcctgccgc cgcgatgggc gatattgagc accttgtcgc agcgaagcat
      601 cgcctgcggg gtcaagcact ttggctccgc gccgccggcg ccaccatcgc cgcccatttc
      661 gccggcaccg ccatcgccgc ccgcgccccc agggctctcg ctgccgctgt cacagccgag
      721 ggccgcaagc gacattgcga tcaagaccaa gataagcttc ttcatggttc ttccctccga
      781 gctcgccttt tggcagccag ctcgcgaact gtcccctcgg ccttgtccgc acgagccggg
      841 cctggcacct ttcgccggtg tcgactcgaa gcgcgtttac gttttgccgc atttgcgagg
      901 ccacctgcgg cctcaaagcc acgatcgagg gcaaccgagt aaccgcggtc gagcccgacc
      961 cggagcatgt ggtgagcaag gggtactcct gtatcaaggg gcttcgctat cacgagatcc
     1021 atcactcgcc cgatcgtctg cgcgtgccgc tcaaacgaat cggagacgtt tttgaagaga
     1081 tcagttggga gcaggcgctc gacgagatag gcgcgaaggt gaagcagctc gtgaaggagc
     1141 atggaggaga ctcgatctcg gcgtatctcg gtaatccgat ctcgttcagc ttgcttccgc
     1201 cgattctaac ctcggggttc ttgcaagggc tcggctcgcg caatcttttt cagaccggct
     1261 cgcaggactg caacaataaa ttcgcggccg ctcagcgggt gtatgggttt ccgttcattc
     1321 aaccctttgc ggacgtcgat cgcacgcagt gcctgatcat catcggctcg aacccagcgg
     1381 tctcccgctc gagcttcatg cagctgcccg atcccgttcg gcggcttcgc gcgatcgaag
     1441 gccgcggcgg cagagtgttt ctcgtcaacc cgaggcgcac cgagaccgcc aagcagctcg
     1501 gcactcaggt gttcattcgc ccagacacgg acgtgtactt ccttctgagc tttctccacg
     1561 aggtgattca acgtgatggc atcgaccatg agcgcgtcgg ccgatacatg cgaggattga
     1621 acgcgctccg gcgcgtcgcc gaaccctggt cgccggaacg aagcgccgaa gtcacaggca
     1681 taccggcgga gacgctccgg gccatggtcg acgcgtatct cgaagccgac ggtgcggcgc
     1741 tgttcctctc gacaggcgtg aaccagggga gccacggcac cctcgcgttc tggattcagg
     1801 aggcgatcaa cgcgattacc ggcaacctcg accggctcgg aggcacgctc gtcgggcacg
     1861 gcttcatcaa ggatttccca aaacatgcgc gcaagggcgg gcatacgatg cgcaaggacc
     1921 tctcgcgcat cggaaagctt ccatcggttg ccgacacgtt tccggcaggg ctcatggccg
     1981 acgagatcct cacacctggg gaaggacaag tgcgcgcgct cttctgcgtc tccggcaatc
     2041 ctctgctcac ggtgccgaac gccaatgggc gactcgaaga ggctttgaag gcactcgagc
     2101 tcctggtggt catcgacatt ttccgcaacg agaccgccaa tcatgcgcac tacattcttc
     2161 ctggcac
//
LOCUS       DASRZF010000422         6909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4789729, whole genome shotgun sequence.
ACCESSION   DASRZF010000422 DASRZF010000000
VERSION     DASRZF010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 6909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6909
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4789729"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VFG22_18665"
     CDS             complement(<1..260)
                     /locus_tag="VFG22_18665"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET6418327.1"
                     /translation="MAQTIPDMLAQRADTLGKTKAIVDGDESLSFFDLAARVDRAARA
                     LIALGIESGDRVAIWAPNIWEWIVAALAIHTVGGVMVPVNTR"
     gene            528..962
                     /locus_tag="VFG22_18670"
     CDS             528..962
                     /locus_tag="VFG22_18670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418328.1"
                     /translation="MIDGTLSPIVLYDMRLPRQVEDEEIALMLSIADFHLDRGLPFIA
                     LVRHARGTGVIPARHRESFADWLELRRERLQRDDVSAVVSLPEAIFRAVLRVVYRFRA
                     PPLRTITTSDIKSAAEAVRKELLRIERPITPEIEALLERLNG"
     gene            1034..1795
                     /locus_tag="VFG22_18675"
     CDS             1034..1795
                     /locus_tag="VFG22_18675"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HET6418329.1"
                     /translation="MGISYEIIDGIAEITVDYPPVNAIPVAGWFELAAILTAAGNNPD
                     TRAVILRAEGRGFNAGVDIKEMQSTEGFESLLGANRGCWEAFKAVYECKVPVITAVHG
                     YCVGGGIGLAGNSDILIAAKGTRFALPEVDRGALGAATHLARLVPPLKMRAMVLTCEP
                     ASAEELHAWGSVYAVVEAEQLAAKAREVAQTFTKKIPRVVRVAKECLNNIDPVDVHRS
                     YRFEQGFTFELNLAGVADEARDAFVEKRDPDSGKK"
     gene            1805..2650
                     /locus_tag="VFG22_18680"
     CDS             1805..2650
                     /locus_tag="VFG22_18680"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HET6418330.1"
                     /translation="MTDKRMTLDEAVADVEDGMTIGIGGWGSRRKPMSLVRALVRKGV
                     KDLTVVSYGGPDIGILCATGQLKKAVYGFVSLDSIPLEPHFRKARQNRALEAVELDEG
                     MFLLGLRAAAQRLPFLPTRAGLGSDVLKINPHLKTVKSPYEDGEQLVAVPALNVDVAF
                     IHMNRADASGNGQALGRDPYFDHLFCMAAKKAYMSCEKIVSTDELVSGGPLQSLVVNR
                     MMVCGMIEAPGGAHFTECLPDYGRDERFQREYAASAKDDEAWDAFRAKYLELSEDEYQ
                     KAVAS"
     gene            2647..3411
                     /locus_tag="VFG22_18685"
     CDS             2647..3411
                     /locus_tag="VFG22_18685"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HET6418331.1"
                     /translation="MSNEEATRADVCTLAVADCFRGDGEIMVSAMGTTPSLGARLAKL
                     TFEPDLLMTDGFNLLLRRALPLGVEPSEGDVEGWMPFGAVFDTLWWGRRHVMMGASQI
                     DQFGNQNIACIGPWEAPRAQLLGVRGAPGNTINHVTSYWVPNHNARALVAKVDMVSGV
                     GYDRAAALGPAGARFHEIRRVVTNLGVFDFDTPDHRMRLRSLHPGVELQQVIDNTGFE
                     LVIPKTLDTSPTPTPDLIAVLRQLDPQGYAQKEVAV"
     gene            3408..4760
                     /locus_tag="VFG22_18690"
     CDS             3408..4760
                     /locus_tag="VFG22_18690"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HET6418332.1"
                     /translation="MTDKKRTTKAPSRERQSSAPKAKRAKKTGAKKRASVAPEARKAT
                     KAVKVIKSKPRKASSKKRRSGAPRSKAEPKSKARKSKSPIRGSSAGQPQPKPAPEPKS
                     ALHTRICELFGIEYPIIQTGMGWVSGASLTSATSQAGGLGILAAATMTFRELEEAIAK
                     VKSRTGNPFGVNMRADQSDIMKRVELLIREQIKVASFAQAPGERVIKTLKDAGVLTMP
                     TIGARRHAEKVQAWGVDAVIAQGQEGGGHTGQIPTSILIPDVSAAVDIPVVGAGGFRD
                     GRGLVAALALGAEGIAMGTRFLLTQESQVPDHVKQVYLDTKANGTVVTKAVDGHPQRV
                     IRTFLIDQLEKASPLTRFPRAALNALSLMKVTGTSLPELLEEGIAMKQNQELTWAQLA
                     MAANAPMLTKASMVDGRIEAGILPTGQVAGVIDEIPTVAELLRRIMTEARETLERLGA
                     "
     gene            4770..5930
                     /locus_tag="VFG22_18695"
     CDS             4770..5930
                     /locus_tag="VFG22_18695"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6418333.1"
                     /translation="MDLDFTESENAFREECRTWLEAHVPREALPSGDTKKGFALHLDW
                     EHQLFDAGWSAVSWPKQYGGREATLFEWLIFEEEYYRLGAPSRVTQNGIFLLAPTLFE
                     FGTEEQKERILRPMARGDVAWGQAWSEPNAGSDLASLKSSARRVDGGWRLSGQKTWST
                     RAAFCDMAYGLFRTDPGQARHRGLTYFMFPLRAEGITVRGVDRLDGDEGFAEIFLEDV
                     FVPDADVIGEVNKGWEVAMATTSSERGLSLRSPGRFMATASRLIDLYHRHRDRSDPIL
                     RDQVIQAWMDAEAYRWFTFQTVTRTVQGESIGPDSSMNKVFWSEMDVRTHETALAIIG
                     DALELDEGSESAVDSGYWMKGFQFALAGPIYAGTNEIQRNIIAERVLGLPRK"
     gene            5935..>6909
                     /locus_tag="VFG22_18700"
     CDS             5935..>6909
                     /locus_tag="VFG22_18700"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6418334.1"
                     /translation="MRFAFTDDQKMLRDTVREVLQRECAPDVVRAAWEGRPERAGAVW
                     KTLAETGVIGMTASEEAGGMAMRELDLVLPVEELGYAAMPGPLVDTVAVGIPLLEAAG
                     TEAQKERWLRPAIAGEARIVVSFEDQPIVAHAASAHVLIAERGGVAYCVPMSEVSVNA
                     ETSVDRTRELGRVSFEATARHQMREDVDAMGLFSMGRERATLGTAAQLIGLSRRMLDM
                     AVQYAKDREQFGKPIGAQQAVKHRLANALIEQEFARPMVYRAGWSMATSAQDAEIHVS
                     LAKIYAGRAARFVAKEALQVHGAIGYTIECDLHMWMKRAWALAAAHGDA"
BASE COUNT         1344 a         2108 c         2224 g         1233 t
ORIGIN      
        1 tagcgggtgt tcacgggcac catcacccca ccgacggtgt gaatcgccaa cgcggcgacg
       61 atccactccc agatgttggg cgcccaaatt gcgacccgat cgccactttc gatacccagc
      121 gcgatcagcg cgcgcgccgc ccggtcgacc cgtgctgcca ggtcgaagaa gctcagcgat
      181 tcgtcgccat caacgatcgc ctttgttttc cccagggtgt cggccctttg ggcaagcata
      241 tcggggatgg tttgggccac cgggctcttg atatcgccaa gccaggatgg gggcaacggg
      301 ccgtcttgga gaattcatct caaccatcgc cgttctcgga ttgaccagcg tcaggatatg
      361 agcatgtttg ccccgatttt gtgggggcct tcctttttgt gaggaactct cacttctttg
      421 actatatgaa tcttcaagcc gcaaatcgcg gctgaacgct ctagtaaaag gcttagaata
      481 tggacaggga ttggagtgta gatccgacct atcggtcgga gcgcttagtg atcgatggga
      541 cactttctcc gatcgtgctt tacgacatgc gactcccgcg tcaggtggag gatgaggaga
      601 tcgcgctcat gctcagcatt gcggacttcc acctcgatcg agggcttccg ttcattgcgc
      661 tcgtgcgaca cgcgcgcggc accggggtga tcccggctcg ccaccgcgag agcttcgccg
      721 actggctcga gttgcgacgg gagcgtctgc agcgcgacga cgtaagcgct gtagtttcgc
      781 tgcctgaggc gatctttcgg gcagtgctga gagtcgttta tcgctttcgt gctccacccc
      841 tgcgaacgat cacgacctcg gatatcaaga gcgcggccga ggcagtccgc aaggagttgc
      901 tccgaatcga gcggcctatc acgccggaga tcgaggcact gctcgaacgc ctgaacggct
      961 gagagctccc tcgtcgactt gacccgccga ctgaaacgcg tttcacttcg gccctgcttc
     1021 gaagggacgc gccgtgggca tttcgtacga aatcattgat ggaatcgccg agatcaccgt
     1081 cgactatcct ccggtcaacg cgattcccgt ggcgggctgg ttcgagctcg cggcgatcct
     1141 gacggccgcc gggaacaatc ccgacacccg agccgtgatc cttcgagccg agggccgggg
     1201 cttcaacgcc ggcgtcgaca tcaaggagat gcagagcacc gagggcttcg aatccctcct
     1261 cggagccaac cgaggctgct gggaagcctt caaagccgtt tacgagtgca aggtccccgt
     1321 catcaccgcg gtgcatggct actgcgtagg gggcggaatc gggctcgccg gcaactccga
     1381 catcctcatt gccgccaagg gcacccggtt tgccttacct gaggtggacc gaggcgcact
     1441 cggcgccgcg acacacctcg ctcggctggt cccgccgctg aaaatgcggg ccatggtcct
     1501 gacctgcgag cccgcgagcg ccgaggagct tcacgcctgg ggttcggtct acgccgtggt
     1561 cgaagcggag cagttggcgg ccaaggcgag agaagtcgct cagacgttca ccaagaagat
     1621 cccacgcgtc gtgcgcgtcg ccaaggaatg cctcaacaac atcgacccag tcgatgtgca
     1681 tcgttcatac cgttttgagc agggcttcac gttcgagctc aacttggctg gcgtcgccga
     1741 cgaggcgcgg gacgcattcg tcgaaaagcg cgaccccgat tccggcaaga agtgaggtag
     1801 cttcgtgaca gacaaacgca tgacgctcga cgaagccgtc gccgacgtcg aagacggaat
     1861 gactatcggc atcggcggat ggggctcacg aagaaagccc atgagcctgg tgcgcgcgct
     1921 ggtgcgcaaa ggggtgaagg acttgaccgt cgtctcctac ggcggaccgg acatcgggat
     1981 tctctgcgcg acaggccagc tcaagaaggc ggtctacggt ttcgtgtcgc tcgattcgat
     2041 tccgctcgaa ccgcatttcc gaaaggcacg gcagaaccga gctctcgagg cggtcgagct
     2101 ggacgagggc atgtttctcc tcggtcttcg cgcggcagca cagcggctgc cgttcttgcc
     2161 cacccgggcg ggtctcggtt cggacgttct gaagatcaat cctcatctaa agacggtgaa
     2221 atcaccctac gaggatggcg agcagctcgt cgcagtgccg gctctgaacg tcgatgtggc
     2281 cttcattcac atgaatcgtg cggacgcatc tggaaacggg caggcgctcg gccgtgaccc
     2341 gtatttcgac cacctattct gcatggctgc gaaaaaggcc tacatgagct gcgagaagat
     2401 cgtttccacg gacgagctcg tgagcggtgg tccacttcag agtctcgtag tcaatcgtat
     2461 gatggtctgt ggcatgatcg aagcccctgg cggcgctcat tttaccgaat gccttccaga
     2521 ttatgggcgt gacgagaggt ttcagcgcga gtacgcggcc agcgccaaag acgacgaagc
     2581 ctgggatgcg ttccgagcga agtacctcga gctcagtgag gacgagtacc agaaggcggt
     2641 ggcatcgtga gcaatgaaga agcgacgcgc gctgacgttt gtactctcgc cgttgcggac
     2701 tgctttcgcg gcgatgggga gatcatggtg agcgccatgg ggaccacgcc gagcctcggc
     2761 gcacgccttg cgaaactgac cttcgagccc gaccttctga tgacggatgg gttcaatctg
     2821 cttctccggc gcgccttgcc gctcggggta gagccgagcg aaggcgacgt ggaaggttgg
     2881 atgccgtttg gggctgtctt cgacacgctc tggtgggggc gccgccacgt gatgatgggt
     2941 gcgtctcaga tcgatcagtt tggcaaccag aacatcgcct gcatcggtcc ctgggaggcc
     3001 ccgagagctc agctactcgg agtgcgcgga gcacctggaa acacgatcaa tcacgtgacc
     3061 agttactggg tgccgaatca caacgcgcga gcgctcgtag ccaaggtcga tatggtgagc
     3121 ggcgtgggtt acgaccgcgc cgccgccctc gggcctgccg gggcccgatt tcacgagatc
     3181 cggcgcgtag tcaccaatct cggcgtgttc gacttcgaca ctccggacca ccgcatgcgg
     3241 ctgaggtccc tgcaccccgg tgtggagctg cagcaagtca tcgacaatac cggattcgag
     3301 ctcgtgattc cgaagacact ggacactagc ccgactccga ccccagacct cattgcggta
     3361 ttgcgtcagc tcgatccgca gggctatgcg cagaaagaag tcgctgtatg acagacaaga
     3421 agaggaccac caaagcgcca agccgagaac gccagtcatc cgcgccgaaa gccaagcgcg
     3481 ccaagaagac aggtgcgaaa aagcgcgctt cggttgcacc cgaggcgagg aaggccacga
     3541 aggccgtgaa ggtgatcaag tcgaagcctc ggaaagcctc gtccaagaaa cgcagatcag
     3601 gcgccccgag atccaaggca gagcccaaga gcaaggcccg caagagcaag tcgcccattc
     3661 gcgggagcag cgcggggcaa ccgcaaccca agcccgcgcc cgaacccaag tcggcgttgc
     3721 acaccaggat ttgcgagctg ttcggcatcg agtacccgat catccagacc ggtatgggct
     3781 gggtgtcggg cgcgagcctc acctcggcga ccagtcaggc gggtggtctc gggattctag
     3841 ccgcggccac gatgacgttc cgggagctcg aggaggccat cgccaaggtc aagagccgca
     3901 cggggaatcc cttcggtgtg aacatgcgcg ccgatcagtc ggacatcatg aagcgcgtcg
     3961 agctcctgat tcgtgaacag atcaaggttg cgagctttgc tcaggcgccc ggtgagcgcg
     4021 tgatcaagac cctcaaggac gccggcgtgc tcaccatgcc tacgatcggt gcgcggcgtc
     4081 atgccgagaa ggtgcaggcc tggggcgtcg atgcggtgat cgcgcagggt caagaaggcg
     4141 gcggccacac cggtcagatt ccaacctcga ttctcattcc ggacgtttcc gcggcggtgg
     4201 acatccccgt cgttggcgcc ggcggctttc gcgacggtcg cggcctcgtc gccgccctgg
     4261 cgctcggcgc agagggcatc gcaatgggga cgcgattcct gctgacccaa gagagccagg
     4321 tgcccgacca cgtcaagcag gtctacctcg acacgaaggc gaatggcacc gtggtgacca
     4381 aggccgtcga tggtcacccg cagcgtgtga tccgaacgtt cctgatcgat cagctcgaaa
     4441 aagccagtcc actcacgcgc ttccctcggg ccgcgctcaa cgcgctgtca ctgatgaagg
     4501 tgaccgggac ttcgctcccc gagctgctcg aggagggcat cgccatgaag caaaatcagg
     4561 agctcacctg ggcgcagctc gcgatggcgg ccaatgcgcc catgctcacc aaggcttcca
     4621 tggtggacgg ccgaatcgaa gcgggcattt tgccgacggg acaggtcgcc ggagtcatcg
     4681 atgagattcc cacggtggcc gagcttctgc ggcgcatcat gaccgaagct cgggagacgc
     4741 tcgaacggct cggtgcctga gcaactgcga tggacctcga cttcaccgaa tccgaaaacg
     4801 ccttccgcga agagtgccgc acttggctcg aagcccacgt gccccgtgag gccttaccgt
     4861 cgggcgacac gaagaagggc ttcgcactgc atctcgactg ggagcaccag ctcttcgatg
     4921 ccggttggtc tgcggtttcc tggcccaagc agtacggcgg ccgcgaagcg acgctcttcg
     4981 aatggttgat cttcgaagaa gaatactatc gtctcggcgc gcccagccgc gtgacgcaga
     5041 acggcatctt cctcctcgcg cccactctgt tcgaattcgg aaccgaagag cagaaggaac
     5101 gcattctccg cccgatggca agaggcgacg tggcctgggg acaggcctgg tcggagccga
     5161 acgcaggcag tgacctcgcc tcgctgaaga gcagcgcgcg acgcgtcgat gggggttggc
     5221 ggctgagcgg ccaaaagact tggtccacgc gggcggcctt ttgcgacatg gcgtacggct
     5281 tgttccgaac cgatcctggg caggcgcggc accgtggttt gacgtacttc atgtttccgc
     5341 tgcgcgccga ggggattacc gtccgtgggg tcgaccgcct cgacggtgac gaagggttcg
     5401 ccgagatctt tctcgaagac gtgttcgtcc cggatgccga tgtgatcggc gaagtgaaca
     5461 agggatggga ggtcgcgatg gcgaccacga gctccgagcg cggcctcagc cttcgcagcc
     5521 ccggtcgctt catggccacg gcatcgcgat tgatcgacct ctaccaccgt catcgggatc
     5581 gctcggaccc gatcctccgc gatcaggtga tacaggcttg gatggacgca gaggcttacc
     5641 gctggttcac ttttcaaacg gtcacccgca cggtgcaagg agagtcgatc ggaccggact
     5701 ccagcatgaa caaagtgttc tggtccgaga tggacgtgcg gactcatgag actgcgcttg
     5761 caatcatcgg cgacgccctc gagctcgatg aagggtccga gagcgccgtc gactcgggct
     5821 actggatgaa gggtttccaa ttcgctctcg ccggcccgat ctacgcgggg acgaacgaaa
     5881 ttcagcgcaa catcatcgcc gagcgagtgc tcggcctccc gcggaagtag cgacatgcgt
     5941 ttcgctttca cggatgacca gaagatgctc cgcgacacgg tacgcgaggt cttgcagcgt
     6001 gagtgcgcgc ccgacgtagt tcgggccgcc tgggaggggc gtcccgagcg agccggcgcg
     6061 gtttggaaga cactggctga gaccggtgtc atcggaatga ccgcttcgga agaggccggt
     6121 ggcatggcga tgcgcgagct cgacctggtg ctgccggtgg aggagctcgg ctacgctgcg
     6181 atgcctggtc cgctggtgga cacggtcgcg gttggtatcc cccttctcga ggccgccggg
     6241 accgaggcac aaaaagagcg ctggctgcga cctgccatcg caggcgaggc ccgcatcgtc
     6301 gtgtccttcg aagaccagcc gatcgtagcg cacgccgcgt cggcccacgt cttgatcgcc
     6361 gagcggggcg gggtcgcgta ctgcgtgccg atgtcggaag tctccgtgaa cgcggaaact
     6421 tcggtcgacc gcacgcgcga gcttggacgc gtttcgttcg aggcaaccgc caggcaccaa
     6481 atgcgcgaag acgtcgatgc gatggggctt ttctcgatgg gccgcgagcg ggcgactctc
     6541 ggcacggcag cgcagctcat aggtctctcg cgacgcatgc tcgacatggc agtgcaatac
     6601 gcaaaagacc gcgagcaatt cggcaagccg atcggcgcgc agcaagcggt aaaacaccgt
     6661 ctcgccaacg cgttaatcga gcaggagttc gcgcggccca tggtctaccg cgcgggctgg
     6721 tccatggcga cttcggcgca ggatgccgaa atccacgttt cccttgccaa gatctatgca
     6781 ggcagggctg caagattcgt ggccaaggag gcgcttcagg tccatggcgc catcggctac
     6841 acgatcgagt gcgaccttca catgtggatg aaaagggcat gggcgcttgc cgcggctcac
     6901 ggggacgcc
//
LOCUS       DASRZF010000423         4944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1352384, whole genome shotgun sequence.
ACCESSION   DASRZF010000423 DASRZF010000000
VERSION     DASRZF010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 4944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4944
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352384"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /locus_tag="VFG22_18705"
     CDS             <1..1066
                     /locus_tag="VFG22_18705"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HET6418335.1"
                     /translation="ADEILMPGQGQVRALFCVSGNPLLTVPNANGRLEEALKALELLV
                     VIDIFRNETANHAHYILPGTSAFQRADLPFVFQSLMGAQGIPYAQFTDALVPTEYEQR
                     DETLILLQLARASGSKLFGSSVANGFFRSWMGLEKLPWIGGRLGLTPQRFLGLMARAF
                     GLGSLKSLRNEPHGRLLPPNRGGDFLGRRVVTDDGLVDLAPEEFVLAAGELQESFELE
                     RAQRDKLKLISKREPLSHNSWMHNHPKFIEGKRSTNYLYMNPEDGEKAGVKSGAMVEV
                     SSSVASVRLPVQLTDEMMPGAVALPHGWGHQAADGLSVASTTSGANVNLLAADGPEEL
                     EYFSGMAKLNGIWVEVVPAD"
     gene            1198..1824
                     /locus_tag="VFG22_18710"
     CDS             1198..1824
                     /locus_tag="VFG22_18710"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET6418336.1"
                     /translation="MIKRWIGEAFLRIAGWKTEGERPTRDRYVIIAAPHTSNWDMPFM
                     LAMAFVYDIPVRWMGKHTLFKAPFGPFFKSLGGIPIVRDRPGGVVGQMVEHFENNDSL
                     VLMVPAEGTRSHVDYWKSGFYHIARAADVPVVLSFLDFGKKVGGIGPSIELTGDFKAD
                     MDKIRAFYADKQGHLPENVGVIRLREEDIEEVIDHEATEEIERLAGNL"
     gene            1901..3859
                     /locus_tag="VFG22_18715"
     CDS             1901..3859
                     /locus_tag="VFG22_18715"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET6418337.1"
                     /translation="MSESSIWLKVLEPGELPEGRVKAVTCKHRTLCLSHFEGEFAALD
                     NKCPHQGGPLGEGSIEKGLLRCPWHGWDFHPITGKPPRDFDDGVETFPVEVRDDGVYV
                     AFPEEQPHVRTSSDVMVETLVNWGIRWVWGMVGHSNLGFADAMRRQEAKGALGYFGIR
                     HEGAASFAASAYGKLTGRPAACFSIAGPGATNLLTGLWDAHVDHAPALAITGQVPTRL
                     IGRGAFQEVDLSAAFGGVAKYTTVAAKSSDFAEISSLACKHAILEQGVSHMVLPDDVQ
                     VIEAPDTEAGSAEGRLAAREISPPEDALSQALERIAAAKRPVIIVGHGARFVMDSVIS
                     LAERLNAPVTTTFKGKGLISDQHPLGCGVLGRSGTPVASWFMNEADLLIVFGASFSNH
                     TGITSYKPIVQIDYDPMMLGRFHSVTVPVWGEIGVTAALMNERLQSVAAIDQRADVVE
                     RWRIWRDEKARRLDETESGGVHSAAIFAALSRTAPEDAIIAVDVGNNTYSFGRYFECR
                     RQSVLMSGYLGSIGFGYPAAMGAWAATQESDRRFRGRKVLAVTGDGGFGQYMAEYTTA
                     VKYGMNITHVLLNNSELGKISKEQRSADYDVWETALHNPSFAEYAELCGALGIRVTEA
                     EQLDGALAEAIAHEGPSTVEVLADALLV"
     gene            3867..>4944
                     /locus_tag="VFG22_18720"
     CDS             3867..>4944
                     /locus_tag="VFG22_18720"
                     /inference="COORDINATES: protein motif:HMM:NF013784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HET6418338.1"
                     /translation="MTVTQLRQQNKIKVASWTSLEDRTPAYALVGDVDLVVLRYDDEV
                     SVLYGRCLHRGALMSDAKVEGENLVCGLHGWDYRYDTGVSSYDNAEVLQKFRAWIDEE
                     ADAVFVDESEIRAWAEKSPQPYDRDQYLGLYADVHGAPEEPFNKHIQELARNGLKNLG
                     HHGPVSAMGVPRQQLPSWEDVLILTAQLARKPLLDDGAVGSAVEIGPRAKKPLRLEIP
                     LFVSDMSFGALSQEAKVALAKGAEMAGTGICSGEGGMLPEEQASNSRYFYELASGRFG
                     WSLDKVKKCQAFHFKAGQGAKTGTGGHLPGNKVTEKIASVRGLNVGEPAISPSRFPDL
                     DRRADRSVVEQRLTSELRGQNQNVL"
BASE COUNT          967 a         1468 c         1598 g          911 t
ORIGIN      
        1 ggccgacgaa atcctcatgc ctggtcaagg acaagtgcgc gcgctcttct gcgtctccgg
       61 caatcctctg ctcaccgtac cgaacgccaa tgggcggctc gaagaggctt tgaaggcgct
      121 cgagctcctt gtggtcatcg acattttccg caacgagacg gccaatcatg cgcactacat
      181 tcttcctggc accagcgcct ttcagcgggc ggacctgccc ttcgtgtttc agtccctgat
      241 gggtgcgcag gggattccct atgcacagtt caccgatgcc ctcgtcccga cggagtatga
      301 gcagagagac gagacgctga ttctcttgca gctcgcccgg gcctctgggt ccaagctctt
      361 cggctcgagc gtcgcgaacg gtttcttcag aagctggatg ggactggaaa agctgccctg
      421 gatcggcggg cggctcggac tcacgccgca gcgttttctt ggcttgatgg cgcgcgcgtt
      481 cgggctgggt tcgctgaaga gtcttcgcaa cgagcctcac gggcgactgc tcccgcccaa
      541 tcgaggaggg gacttcctcg gacggcgcgt cgtcaccgac gacggtttgg ttgatttggc
      601 acccgaggaa ttcgtgctgg ccgcgggcga gctccaagaa tccttcgagc tcgagcgtgc
      661 acaacgcgac aagctcaagc tgatcagcaa gcgagagccg ctcagtcaca atagctggat
      721 gcacaatcac ccaaagttca tcgaaggcaa gcgctcgacc aactacctct acatgaaccc
      781 cgaggatggt gagaaggcag gtgtgaaatc aggcgccatg gtggaggtga gcagcagcgt
      841 cgcctcggtg cgcctccccg tgcagcttac cgacgagatg atgccgggcg ccgtcgcact
      901 gccgcacgga tggggacatc aggccgccga tggactgagc gtggccagca cgacctcagg
      961 cgccaacgtc aatctcctgg ccgccgacgg tcccgaagag ctcgagtact tttcgggaat
     1021 ggcgaagttg aacggcatct gggtcgaagt cgttccggca gactaggtca gcagtgaccg
     1081 gcgtcggcgt cgggaccttt ccgattgtaa aagttctgtc accaattcag gcatttctcg
     1141 taacccgcgc cgcggtctgc aatacaaagg acagctggcc gtaggttggc gtgcgcaatg
     1201 atcaagcgat ggataggcga agcgttcctt cggatcgcag gttggaagac cgagggtgag
     1261 cggccgactc gagatcgcta cgtcatcatc gcagcaccgc acacctcgaa ctgggacatg
     1321 ccgttcatgt tggcgatggc gttcgtctac gacattcccg ttcgatggat gggtaagcat
     1381 accttgttca aggcgccgtt cggtccgttc ttcaagagcc tcggcggtat tccgatcgtg
     1441 cgtgatcgtc ctggaggcgt cgtcggacag atggtcgagc acttcgagaa caacgactcg
     1501 ctggtcctga tggttccggc cgaagggact cgctcgcacg tcgactattg gaagtccggt
     1561 ttctatcaca tcgcccgcgc agcggacgtt ccggtcgtct tatcctttct cgactttggc
     1621 aagaaggtcg gaggcatcgg accgtcgatc gagctcacgg gcgacttcaa ggcggacatg
     1681 gacaagatcc gcgcattcta cgccgacaag caggggcatc ttccggagaa cgtcggtgtg
     1741 atccgtcttc gcgaggaaga catcgaagag gtgatcgacc acgaggcgac cgaggaaatc
     1801 gagcggctcg caggcaatct gtaacatccc ggctccctga acgtccttta cagctccgag
     1861 ccgatcgtgg aaggctccgg acttcgcgaa aggcgttcga atgagtgaga gcagcatttg
     1921 gctcaaggtt ctcgagccgg gggagcttcc cgaaggccgc gtcaaagcgg tcacctgcaa
     1981 gcatcggacg ctctgcctga gccatttcga aggagagttt gctgcgctcg acaacaagtg
     2041 cccgcatcag ggcggtccct tgggtgaggg ctccatcgag aaaggcctgc tgcgttgccc
     2101 ctggcatggc tgggacttcc accccatcac cggaaagccg cctcgcgatt tcgatgacgg
     2161 tgtcgagacg ttcccggtcg aggtgcgcga cgacggcgta tacgtcgcgt ttcccgagga
     2221 acagccacac gttcgaacca gcagcgacgt catggtcgaa acactggtca actggggcat
     2281 tcgctgggtc tggggaatgg tagggcattc gaatctcggc tttgctgatg cgatgcgaag
     2341 acaagaggcg aagggcgcgc tcggttactt cggcatccga cacgagggcg cggcttcgtt
     2401 cgccgcttct gcgtatggga agctcaccgg acgtcccgcc gcctgcttct ccatcgcggg
     2461 gcccggtgcg accaatctcc tcacggggct ttgggatgcg cacgtcgatc atgctccggc
     2521 gctcgcgatc accgggcagg ttcctacacg cctcattgga cggggtgcct ttcaagaagt
     2581 ggatctcagt gcagcgttcg gcggcgttgc gaagtacacc acggtggctg ccaagagctc
     2641 cgacttcgcg gagatcagca gtcttgcctg caagcatgcg atcctcgaac agggcgtctc
     2701 gcacatggtt cttcccgacg acgtacaggt catcgaggcg cccgacacgg aggccgggtc
     2761 agccgaaggc agattggcag cgcgagaaat ctccccgccc gaggacgcgc tcagccaagc
     2821 gctcgagcga atcgcggcgg cgaaaaggcc cgtcatcatc gtggggcatg gcgcacgctt
     2881 cgtgatggat tcggtcatct cgctcgccga gcggctcaat gcacccgtga cgaccacatt
     2941 caagggcaag ggtctgatct ccgatcagca tccgctcggc tgcggagtgc tcgggcgcag
     3001 cgggaccccg gtggcgagct ggttcatgaa cgaggccgac ctgctcatcg tgtttggcgc
     3061 gagcttcagc aatcacacgg gcatcacgag ctataagccg atcgtccaga tcgactacga
     3121 tccgatgatg ctcggcaggt ttcactcggt gaccgtgccc gtctggggcg aaataggcgt
     3181 gacggcggcg ctgatgaatg agcggctcca gtcggttgcc gcgatcgatc aacgcgctga
     3241 tgtggtcgaa cgttggagga tctggaggga cgagaaggcg aggcgtctcg acgaaaccga
     3301 gagcggcggc gtccattccg cagcgatctt cgcagcgctc agcagaaccg ctcccgagga
     3361 tgcgatcatc gcggtcgacg tggggaacaa cacctattcg ttcggccgct atttcgaatg
     3421 ccggcgacaa tcggtcttga tgagcggtta tctaggctcg atcgggttcg gataccccgc
     3481 agccatgggc gcttgggccg ccactcagga gagcgatcgg cgctttcgcg gtcgtaaagt
     3541 attggccgtg acgggcgacg gaggtttcgg tcaatacatg gcggagtaca cgacagcggt
     3601 gaaatacggc atgaacatca cgcacgtcct tctcaacaac agcgagctcg ggaagatcag
     3661 caaagagcag agaagcgccg actacgacgt gtgggagacg gcgcttcaca accctagctt
     3721 cgccgaatac gccgagctct gcggcgcgct cggcatacgg gtgaccgaag cggagcaact
     3781 ggacggcgcg cttgcagagg cgattgcgca cgagggcccg tcgaccgtcg aggttctcgc
     3841 cgacgcactg ctggtataag agagccatga ccgtgacgca gctacgacag caaaacaaaa
     3901 tcaaggtcgc gagctggacg tctctcgagg accgaacgcc cgcgtacgcg ctcgtcggcg
     3961 acgtcgactt ggtcgtgctg cgatacgatg atgaggtctc ggtcttgtac ggacgctgct
     4021 tgcaccgcgg cgccctgatg tccgacgcca aagttgaggg ggagaacctc gtctgtggcc
     4081 tgcacggctg ggactatcgc tacgacacgg gcgtgagctc ctatgacaac gcggaagtgc
     4141 ttcagaagtt tcgcgcctgg atcgacgagg aagcagacgc cgtgttcgtc gatgagtccg
     4201 agatccgcgc ctgggcggag aagtctccac agccctacga tcgggatcag tatttgggcc
     4261 tttacgccga cgtgcacggg gcccccgagg agccgttcaa caagcacatc caagagctcg
     4321 cgcgcaatgg tttgaagaac ctggggcacc acgggccggt ctcggcgatg ggggtgccgc
     4381 ggcagcagct cccctcctgg gaggacgtct tgattctgac cgcgcagctc gctcgtaagc
     4441 cgctgctcga cgacggagcg gtcggctcgg cggtcgagat cggaccgcgg gcaaagaagc
     4501 cgctccggct cgagatcccc ctcttcgtca gcgacatgag cttcggcgcg ctgagccaag
     4561 aggcgaaggt cgctctggcc aagggcgccg agatggcagg gaccggcatc tgctcgggcg
     4621 agggcggaat gcttcccgag gagcaagcga gcaattcgcg ctacttctac gagctggctt
     4681 cagggcgttt cggctggagt ctcgacaagg tgaagaagtg tcaggcgttt cacttcaagg
     4741 ccgggcaggg cgcgaagacc gggaccggtg ggcatctgcc cggcaacaag gtgactgaga
     4801 agatagcctc ggttcgtggg ctcaatgtcg gagagccggc gatcagccca tcgcgctttc
     4861 ccgatctcga ccgccgagcc gaccgctccg tcgtcgagca gcggcttacg agcgagctgc
     4921 gcggtcagaa tcaaaacgtc ctcc
//
LOCUS       DASRZF010000424         3629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2387318, whole genome shotgun sequence.
ACCESSION   DASRZF010000424 DASRZF010000000
VERSION     DASRZF010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2387318"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VFG22_18725"
     CDS             <1..582
                     /locus_tag="VFG22_18725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418339.1"
                     /translation="EDAPETGPDWRALDRDEPAANAVNDRAPEMAERIERQADGKFLL
                     TNSKSEREIRAEPQDPPPEQNTATVSDYFRAVDAVRSEQGAGDPNTFAMGLIKAGLGG
                     STAGFDQLIDDTERMELEMKQIRPAPGCEQYHQASIEALAEGRELLDDLKTAISERDI
                     QGLTTMARRAGELEAKAKSMDAMRAELEAGAHP"
     gene            complement(612..1181)
                     /gene="infC"
                     /locus_tag="VFG22_18730"
     CDS             complement(612..1181)
                     /gene="infC"
                     /locus_tag="VFG22_18730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HET6418340.1"
                     /translation="MAIARPPFGARERPRNFGPRINDRIRVPEIRVIGADGGMLGVMN
                     TDEARRMARSLDLDLVEVNPKASPPVCKIMDFGRFKYEEKKRQAETRKRQTQIELKEI
                     KLRPKTDEHDLEFKTKHVRRFLEEGNKVKITVRFRGREITHPETAQRQIEAIVQAVAD
                     IGAVETSARLEGRAMTAILSPRVKVTHAR"
     gene            complement(1168..3162)
                     /gene="thrS"
                     /locus_tag="VFG22_18735"
     CDS             complement(1168..3162)
                     /gene="thrS"
                     /locus_tag="VFG22_18735"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HET6418341.1"
                     /translation="MTNQATARTARELLEERSALAKDTVAARVDGKLYDLLTPLPDGV
                     SEVKPIGAHDPDALAVIRHSSAHVMADAVQRLFPGTKVAFGPATEDGFYYDYARPDGS
                     FSEEDLRAIEEKMAEIVEADSPFRREVVTRDEARALLQSLDEGFKLEHLERLEDPISL
                     YRHGDWVDLCEGPHVPSTGFLKAVKLTSVAGAYWRGDERNPMLQRIYGTAFASPKALR
                     AHLKQLEEAKQRDHRKLGKELKLFAFHPHAPASPFFLPRGSTIYNLLEAYVRRLYARY
                     GYEEVITPQIFDNELFHTSGHLPSYAENMYLAATSENLEKLQKRVEEKPPQSPDDWAE
                     VFNESLRFGVKPMNCPSHCLMYGMTKRSYRDLPMRMADFGRLHRFERSGVVQGLTRVR
                     TFAQDDAHIFCTLEQVQDEIKSFLDLVYEAYRDFGFTDVRIVIATRPEQRLGADEVWD
                     QSEEALEEAVKAKELPYEIAEGEGAFYGPKIEFHLKDAIGRPWQLGTIQADFNLPERF
                     DLSYVGDDNTTHRPVMLHRAILGSIERFFGVLTEHVGGSFPVWLAPEQIALLTVSEKF
                     NEYAEHVQHELQERGFRVTADLSSDKLGAKIRQARMMRIPYLGVIGQQEVEGQGLALR
                     SRDENKDLGFIPLSEVIERLTSENQPPSSPVSTTRGDSHR"
     gene            complement(3266..>3629)
                     /locus_tag="VFG22_18740"
     CDS             complement(3266..>3629)
                     /locus_tag="VFG22_18740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418342.1"
                     /translation="VQRAGAAIERNATRHYTGMAFATLASRALDISLYDTQCGAKLFA
                     RDLVPGLFSEPFLTRWLFDLELLMRLRRLLGREEFDRIVYEHPLSVWVEKGDSRIRLR
                     DALAVPYDLARIRRAYRL"
BASE COUNT          653 a         1102 c         1185 g          689 t
ORIGIN      
        1 gaggatgccc ctgaaacggg gccggactgg cgggcgctcg accgggacga gccggccgca
       61 aacgcggtga atgacagggc cccggagatg gccgagcgca tcgagcggca agcggacgga
      121 aagttccttc tcaccaacag caaaagcgag cgagagatcc gggcagagcc gcaagatcct
      181 ccgccggaac agaacacagc cacggtatcc gactacttcc gggcggtcga cgcggtccgt
      241 tcggagcagg gtgcaggcga tccgaacacg ttcgcgatgg gcctcatcaa agctgggctt
      301 ggaggttcta ccgcggggtt cgaccagctc atcgacgaca ccgagcgcat ggagctcgag
      361 atgaagcaga ttcgcccagc gccggggtgc gagcagtatc atcaggcgag catcgaagcg
      421 cttgccgaag ggcgcgagtt gctcgatgac ttgaagacgg cgatctcgga gcgagacatc
      481 caggggctca cgacgatggc acggcgagcc ggagagctcg aagccaaggc caagtcgatg
      541 gacgcgatga gagcggagct cgaagcgggc gcccaccctt aggcgcatgc cgcttcgggg
      601 cgaggtgcag gttagcgggc gtgggtgact ttgacccgcg gactcaggat ggctgtcatg
      661 gcgcgaccct cgagtcgggc gctcgtttcg acggcgccga tgtccgcgac cgcctgcacg
      721 atggcctcga tctgacgttg cgccgtttcc ggatgcgtga tctcacgccc gcggaagcgg
      781 acggtgatct tcaccttgtt gccctcctcc aagaagcggc gcacgtgctt cgtcttgaac
      841 tcgagatcgt gttcgtcggt cttcgggcga agcttgatct ccttgagctc aatctgcgtc
      901 tgcctctttc gggtctcggc ttgcctcttc ttctcttcgt acttgaagcg gccgaagtcc
      961 atgatcttgc agacgggcgg gctggccttc gggttcacct cgaccaggtc gaggtcaaga
     1021 ctccgcgcca tccggcgagc ctcgtcggtg ttcatgacac caagcatgcc tccgtcagct
     1081 ccgatgactc ggatctcggg gactcgaata cgatcgttga tacgcggtcc aaaattgcgc
     1141 ggacgttcgc gcgctccaaa gggcggtcta gcgatggcta tctcctcttg ttgtagaaac
     1201 gggtgacgaa ggcggctggt tctcgctcgt gagacgctcg atgacttcgg acagcgggat
     1261 gaaccctagg tccttgttct cgtctcgcga gcggagcgcc aggccctgcc cttcgacttc
     1321 ttgttggcca atcacgccga gatacggaat ccgcatcatg cgcgcttggc gaatctttgc
     1381 gcccagcttg tcgctgctga gatcggcggt gacccggaag cctcgttctt ggagctcatg
     1441 ctgcacgtgc tcggcatact cgttgaactt ctcactcacc gtcaacagcg cgatctgttc
     1501 tggagccagc cagaccggga aggagccccc gacgtgctcg gtcagcaccc caaaaaaccg
     1561 ctcgatggac cccaaaatcg ccctgtggag catcacgggc cggtgagtcg tgttgtcgtc
     1621 gcccacgtag ctgaggtcga agcgctccgg caggttgaag tctgcctgga tggtgccgag
     1681 ctgccaggga cgcccgatcg cgtccttcaa gtggaactcg atcttcgggc cgtagaaggc
     1741 gccctccccc tcggcgattt cgtagggcag ctccttggct ttgaccgctt cttcgagggc
     1801 ttcctcggac tggtcccaga cttcgtcggc gccgagtcgc tgctcgggcc gcgtcgcaat
     1861 cacgatgcgc acgtcggtga acccgaaatc acggtacgcc tcgtacacga gatccaagaa
     1921 gctcttgatc tcgtcctgca cctgctcgag cgtgcagaag atgtgcgcgt cgtcctgggc
     1981 aaacgtacga acacgcgtga gcccttggac cacgccgctt cgctcgaagc gatgcaggcg
     2041 tccaaagtcg gccatgcgca tcggaaggtc gcgatagctt cgcttggtca tgccgtacat
     2101 gaggcagtgg ctcgggcaat tcatcggctt cacaccgaag cggagcgact cgttgaacac
     2161 ctcggcccaa tcgtcggggc tctgtggcgg cttttcttcg acacgcttct ggagcttctc
     2221 gaggttttcc gaggtggccg ccaggtacat gttctcggcg tacgaaggga gatgtcccga
     2281 tgtgtgaaag agctcgttgt cgaagatctg cggcgtgatg acctcttcgt acccgtaacg
     2341 cgcgtagagc cggcgaacgt aagcctcgag cagattgtag atggtcgagc cgcgcggcag
     2401 gaagaacgga gaggccggcg cgtgggggtg aaacgcgaag agcttgagct ccttgccgag
     2461 cttgcgatgg tcgcgctgct tggcttcttc gagctgcttg agatgcgcgc gcaaagcctt
     2521 gggcgaagcg aaggccgtcc catagatgcg ctgaagcatc gggttgcgct cgtcccctct
     2581 ccagtacgca ccggcaacac tggtgagctt gacggctttc agaaaaccgg tggacggaac
     2641 gtgcggcccc tcgcagaggt ccacccagtc gccgtgcctg tagagcgaga tcgggtcttc
     2701 caagcgttcg aggtgctcga gcttgaaacc ctcgtcgagc gattgcagga gcgcacgcgc
     2761 ctcgtcacgg gtcacgacct cgcggcgaaa cgggctgtcg gcttcgacga tctccgccat
     2821 cttttcttcg atggcgcgaa ggtcttcttc cgaaaacgat ccatccggcc gcgcatagtc
     2881 gtaatagaag ccgtcctcgg tggcgggacc gaaggcgacc ttggtgccag ggaagagccg
     2941 ctgcacggcg tcggccatga cgtgcgcgct cgagtgccga atcaccgcga gggcgtcggg
     3001 atcgtgcgcg ccgatcggct tcacttcgga gacgccgtcg ggaagcggtg tcagcagatc
     3061 gtacagtttc ccgtcgaccc gggcggccac ggtgtccttg gctagcgcgc tgcgctcctc
     3121 gagcagctcg cgcgcggttc gcgctgtcgc ttgattcgtc atcggtcggt ggaccgaagt
     3181 atgtagctcg cgctcaagac cagtgttctc caggacgtcg gccacggggt tagctcatgg
     3241 cgccccggtg tgcaattcgc gggcctcaga gcctataagc ccgtcgaata cgagccaggt
     3301 catagggaac ggccagggcg tctcgcagtc ggatcctcga atcgcctttt tcgacccaga
     3361 cgctcagcgg atgctcgtag acgattcggt cgaactcctc ccgcccgagc agtctccgta
     3421 ggcgcatcag gagctcgagg tcaaagagcc agcgcgtgag gaacggctcg gagaagaggc
     3481 caggtacgag gtcgcgcgcg aacaacttcg cgccgcattg cgtgtcgtag agtgaaatat
     3541 cgagggcacg cgacgcgagc gtggcgaacg ccatacccgt gtagtggcgc gtggcgttgc
     3601 gctcgatggc ggcgccagcg cgttggacg
//
LOCUS       DASRZF010000425         3107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1704774, whole genome shotgun sequence.
ACCESSION   DASRZF010000425 DASRZF010000000
VERSION     DASRZF010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1704774"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VFG22_18745"
     CDS             complement(<1..234)
                     /locus_tag="VFG22_18745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418343.1"
                     /translation="MIQARRLMLLVLFLACMINLFSAADELPKIRISLSPVMSSAALA
                     FAEGWGLFEKHGLNVELVGLSDDEERSAALMAGD"
     gene            366..438
                     /locus_tag="VFG22_18750"
     tRNA            366..438
                     /locus_tag="VFG22_18750"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:400..402,aa:Glu,seq:ctc)
     gene            complement(504..944)
                     /locus_tag="VFG22_18755"
     CDS             complement(504..944)
                     /locus_tag="VFG22_18755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418344.1"
                     /translation="MVHDRPRIRRRIEALIWRRRLCSCTGYSGAPSGASWHGTSIDGV
                     ERSAELTPIDVPLPEIRESDVAFWFDADGDEPTCQRVAPHAKLMSETDLSFPIILSQD
                     GRVMDGMHRLCKALIEGRDTIQAVQFEVDPEPDYVSVPSDELPY"
     gene            1007..2830
                     /locus_tag="VFG22_18760"
     CDS             1007..2830
                     /locus_tag="VFG22_18760"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET6418345.1"
                     /translation="MALLAARNLSKTHASSTLFDRLSITFHPGERVGLIGPNGGGKTT
                     LLKILAGAERADSGVVERSKDARLAYLPQVDVFPADATVESTLLDAVRSVELEEYEYT
                     VRARKMLRRLGFETGDQPVDTLSGGWRKRLAIGCALVQEPDLLLMDEPTNHLDLAGIE
                     WLERFLARSKLSFILTSHDRYFLERVANRIVEINPRYPDGFLSVNGHYSDFLEKREAF
                     LQMQDAKRQALANEVRREVEWLRRGPKARSSKAGYRIDEAHRKIGELSKAKRRHDSET
                     EVGIDFRASGRRTHDLITVHGIEKSLGGRLLFRGLDAHLCPGTRLGITGNNASGKTTL
                     LKTLAGTLPPDDGHVKHATDLRIALFDQQRSQLDESETLRRALAPSGDTVSYLGRASH
                     VVAWAKRFGFAPDQLEMPVGELSGGEQARVLMAMMMREPADVLMLDEPTNDLDIASIE
                     VLERALDDFPGAVIVITHDRHLLDRICTELIGLHGDGRWGAYASVAQWLDVGAAAEKP
                     SPATGRALERSRKVVSDSGAGSPRKGLTYHEKQEFADMEERILAAEEKLDALQAQLND
                     PDVAGDHERLHATYEAYREAQQELDRLFARWEELEGKALGK"
BASE COUNT          627 a          992 c          948 g          540 t
ORIGIN      
        1 atctccggcc atcagcgccg cgctgcgctc ttcatcgtca ctcagcccca ccagttccac
       61 gttgagaccg tgtttctcga acagccccca tccttcggca aaggcaagag cagcagaact
      121 catgactgga gagaggctga tgcgaatctt gggcagctcg tcggccgcgg agaacagatt
      181 gatcatacaa gctagaaata gaacaagcag catcaggcgg cgagcctgga tcatgatatt
      241 cccccctatc tgtgaaccgt atgggtcgat tatactgtgc ttattgtcgg tgacaaaagg
      301 gtggatcttg cctccgcgtg cttgatgcag agtgggcgat tctggataat ttccagttga
      361 ctatcggtcc cgtcgtctag tggcctagga tatcgggctc tcatcccggc gacaggggtt
      421 cgaatcccct cgggaccaga aggccaagac gcaagacctt gtacctggcc caggatgggc
      481 agccacgcgc atcgctggcg agcctagtac ggaagctcat cggaagggac actgacgtag
      541 tccggttccg ggtccacctc gaactggacg gcctggatcg tgtcccttcc ttcgatcagt
      601 gccttgcaca agcgatgcat cccgtccatc acccgcccgt cttgcgacag gatgatcggg
      661 aagctcagat ccgtctcgct catcagcttg gcgtgaggag caacccgttg acaagtcggc
      721 tcgtcgccgt cggcatcgaa ccagaacgcc acatccgact cgcgaatctc cggcagcggc
      781 acgtcgatcg gcgtgagctc cgccgagcgc tcgaccccat cgatcgacgt cccatgccag
      841 gaggccccac tcggagcacc ggaatagcct gtgcaactgc ataatcttcg ccgccagatc
      901 agggcctcga ttcgtctccg aatgcgtggc cgatcgtgta ccatctggac ccatccggcc
      961 ggtcgagcct cttgtaggtc cggccgcccc cgaaggagcg tggacgatgg ccctgcttgc
     1021 ggcacgtaat ctctcgaaga cgcacgcctc gagcaccctg tttgaccgcc tatcgatcac
     1081 gtttcacccc ggcgagcgcg tcgggctgat cggaccgaac ggaggcggca aaacgacgct
     1141 gctgaagatt ctcgccgggg ccgagcgcgc cgactccggg gtcgtcgagc gttccaagga
     1201 cgcgcgcctg gcgtatcttc ctcaagtcga cgtctttccc gccgacgcca ccgtcgagag
     1261 cacgctgctc gatgccgttc gatccgtcga actcgaggaa tacgagtaca ccgtacgagc
     1321 acgcaagatg ctgcggcgcc tcggctttga gaccggcgac caaccggtcg ataccctttc
     1381 cggcggatgg cgcaagcggc tggccatcgg ttgcgccctg gttcaggagc ccgacctgct
     1441 attgatggac gagccgacga accatctcga cctcgcgggc atcgagtggc tggagcgctt
     1501 cctcgctcga tctaagctct ccttcatcct gaccagccac gaccggtact tcctcgaacg
     1561 tgtcgccaat cgcatcgtgg agatcaaccc tcgctacccc gatggcttct tgagcgtcaa
     1621 cggccactac agcgatttcc tcgagaaacg cgaagcgttc ctccagatgc aggatgcgaa
     1681 gcggcaggcg ctcgcgaacg aagtccggcg agaggttgag tggctgcgcc gagggcccaa
     1741 ggcacgatcg agcaaggccg gctaccggat tgacgaagcc catcgcaaga tcggcgagct
     1801 gtcgaaggcc aagcgccgcc acgacagcga aacggaagtt ggcatcgact ttcgcgccag
     1861 cgggcggcga acgcacgacc tgatcaccgt gcacggcatc gagaagagcc tcggcggccg
     1921 cctgttgttc cggggactcg atgcgcatct gtgccccggc acacggctgg gaataaccgg
     1981 caacaacgcc agtggcaaga ccacgctgtt aaagacactg gctgggaccc tgccgcccga
     2041 tgacgggcac gtcaagcacg ccaccgacct acgcattgcg ctgttcgatc agcagcgaag
     2101 ccaactcgac gaaagcgaga cgcttcgccg ggcgttggcc ccgagcgggg acacggtctc
     2161 ctatctcgga cgggcgtccc acgtcgtcgc ctgggcgaag cggtttgggt ttgcgcccga
     2221 ccagctcgag atgccggtgg gggagctctc cggtggagag caggcgcgtg tgttgatggc
     2281 gatgatgatg cgcgagccgg ccgacgtgtt gatgctcgac gagccgacca acgatctcga
     2341 catcgcgtcg atcgaggtgc tggagcgtgc gctcgacgac ttccccggtg ccgtcattgt
     2401 catcacccac gatcgccatc tgctcgaccg gatctgcacc gaactgatcg gactccacgg
     2461 tgacggccgc tggggcgcct acgccagcgt ggcccagtgg ctggatgtcg gcgcagccgc
     2521 agagaagccg tccccggcca ctggaagagc gctggagcgc agccgcaaag tggtttccga
     2581 ttccggcgcc ggctccccgc ggaaggggct gacctaccac gagaagcagg agttcgccga
     2641 catggaagag cgcatcctcg ccgccgagga gaagctcgat gcactacagg cacaactgaa
     2701 cgaccccgac gtggccggcg atcacgaacg gctacacgcc acgtacgagg cgtatcgaga
     2761 agcccaacag gagcttgatc ggctgttcgc ccgttgggaa gaactggagg gcaaagcgct
     2821 cgggaagtag gcggtccagt tgggcaggct gcccagtcac agctgaatct ggggccttct
     2881 ctttcggaga tcccgacgaa tgtttcgaac agccgaacca tgaatcttca atcagacggg
     2941 agtgaaactt gcaattggcc gaaggcaaaa gttacaatca gccgacatgg ttcccgacgt
     3001 gctttacccc cgattcgtgg agacatcgct cgcggaagcg gtatccgaca cgcccgtggt
     3061 gctcattcac ggaccacggc aatgcggcaa gaccacactg gccaaga
//
LOCUS       DASRZF010000426         3794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1356708, whole genome shotgun sequence.
ACCESSION   DASRZF010000426 DASRZF010000000
VERSION     DASRZF010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1356708"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VFG22_18765"
     CDS             complement(<1..197)
                     /locus_tag="VFG22_18765"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HET6418346.1"
                     /translation="MIRLPEDCKLAFCVGVGGVGKTTFAAALALREALNGRSVLVLTA
                     DPARRLADALGMHALRDAPSR"
     gene            complement(194..1120)
                     /locus_tag="VFG22_18770"
     CDS             complement(194..1120)
                     /locus_tag="VFG22_18770"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HET6418347.1"
                     /translation="MHAPNGEPLRRPVQFFTGKGGVGKSTVLGAVATAAARAGKRPLI
                     LELGIHTSSSHLFSSPDVGYVPAEVAPGVMASRVEFEPALVDYITSRLKLRPIATMVA
                     NNSSLRRLFLAAPGVDEIVTLHRVAQLVEDARWDLILVDLESTGHALMFFDLPSVLEV
                     FLKDGPLRQVLDSATKLVRDVDRSAIHIVTVPEPLAVNETILLYRRLLARDDLHLGHL
                     FVNRYPPIWFDEEEQRLLHAELSGPSRGQRWAADLELAEYLVRRRETADKCLRGLRRD
                     IDLPTTVFDAQDQGSAAIIEALSRAIELSEVW"
     gene            1169..2026
                     /locus_tag="VFG22_18775"
     CDS             1169..2026
                     /locus_tag="VFG22_18775"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HET6418348.1"
                     /translation="MKSWIQASRPLAHVNIAVPLVLGQVAAWHVTARFSWRWFAAAFL
                     WGLLDHLFVIFSNDFADREADSVQRTILSGGSGVIAEGRLSPHQVKRAARGAAISLIA
                     YSCLLAVAGRGWTPVYGLAAILLMWLYSFPPVRLSYRGGGELLQGIGMGIGLPSLGYY
                     LQTEGPFAPGWVIWPAALLGVCSNVLTALPDVENDARAGKRTWPVRLGIRSARRFGSA
                     GIAFAAFGVFLWTPGVPVPAKALLSIAPLLPLLVAARDEEPFHAAWWSSIALNLLLAL
                     WMLAMIVPL"
     gene            complement(2023..2775)
                     /locus_tag="VFG22_18780"
     CDS             complement(2023..2775)
                     /locus_tag="VFG22_18780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418349.1"
                     /translation="MAAIDTHMTAHATNHIGATLAEIDERIGLTAYGRLTLDPCRSTA
                     NRLDDLVMGLTDPGETPHLGAAAVAVADAQLRSFPENLFWDFDFFLASIHRDALASPD
                     YADHLRKVTRVTVGLMNLYGQQSAIRFRYVHDFMYGFDWARWVRRDPGSRQEAAPFGM
                     EFLQQSENRGRDILKLIDEDDALYPSLEDGVARNPFPFAREPEHELPLYRLLAERGEV
                     PVETWRFDARPDSSRDFDALREEAAACLGLSR"
     gene            complement(2751..3710)
                     /locus_tag="VFG22_18785"
     CDS             complement(2751..3710)
                     /locus_tag="VFG22_18785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HET6418350.1"
                     /translation="MRTSFTSDLPPESRLERWAYVLKPASWPKLFVPALFGQALGASE
                     KGALGLEALGWGFAFTLFGLGFIVLLNDWGDREVDAIKRNMFPDGCSPKTIPDRLIDS
                     RAIAIAGVLFGAATLLVAAGAERFLLRPFAFEAGLACMLLFVAYTLPPIRLNYRGGGE
                     LLEMAGVGLALPVYNAYLQAGSVALAAWPWILGFSALSLASGVASGLSDEESDRVGGK
                     RTFASMRGNAAARALTEALVVLGAGVWLAGSIAAPSRAPFYVSASAVLIVSWNFAAMR
                     RVSKSAATNAFREQAEYKRFLHRAIWHSTPLVAVLLWLRSILT"
BASE COUNT          703 a         1220 c         1175 g          696 t
ORIGIN      
        1 atccgcgagg gagcatcacg caacgcatgc attcccagcg cgtccgcgag gcgccgcgct
       61 ggatcggccg tcaagacgag caccgacctg ccgttgagag cctctcgcag tgccaatgcg
      121 gccgcaaaag tcgtcttgcc aaccccaccg acacccacgc agaacgcgag cttgcaatcc
      181 tcgggtagcc tgatcaccag acctccgaca gctcgatcgc tctcgagagt gcttcgatga
      241 tcgcggcgct tccctgatcc tgggcgtcga agacggtcgt gggtaggtcg atgtcgcggc
      301 gaagcccgcg taggcacttg tcggccgtct cccttcgccg caccaggtat tccgcaagct
      361 ccagatccgc ggcccagcgc tgcccgcggc tcggaccgct caactcggca tggagcagtc
      421 gttgctcctc ctcgtcgaac cagatcggcg ggtagcggtt gacgaagaga tggccgaggt
      481 gcaggtcgtc tcgtgctagc aggcgccggt acaagaggat cgtttcgttg acggcaagag
      541 gttcgggcac cgtcacgatg tgaatcgccg agcgatcgac gtctcgaacg agcttcgtcg
      601 cgctgtccaa gacctggcga aggggcccat ccttgaggaa tacctcgaga acgctgggaa
      661 gatcgaagaa catcagtgcg tgtcccgtcg actcgaggtc gacgagaatc aggtcccagc
      721 gggcgtcttc gacgagctgc gccactcggt gcagcgtcac gatctcgtcg acgccgggcg
      781 ccgccaggaa cagccgtcgg agcgagctgt tgtttgcaac catggtcgcg attggacgga
      841 gcttgaggcg cgaggtgatg tagtcgacga gcgcaggctc gaactcgaca cgggatgcca
      901 tcaccccggg ggccacctcg gcaggcacgt atccgacgtc cgggctcgaa aacaagtgcg
      961 acgaagatgt atggatgccg agctccagta tcagtggacg ctttcctgcg cgcgccgccg
     1021 ctgtggccac ggcgccgagt acggtcgact tgccgactcc accctttccg gtgaagaact
     1081 ggacgggccg cctgagtggt tccccgttgg gcgcgtgcac ctcgcatccc tagcaggatc
     1141 gaaacgtgat atcgagtgac ctgaccgtat gaagagctgg attcaagctt cgcgtccact
     1201 cgcgcacgtc aacatcgccg tgccgctcgt gctgggacaa gtagccgctt ggcacgtcac
     1261 cgctcggttt agctggcgat ggttcgctgc cgctttcctc tggggactct tggatcacct
     1321 cttcgtgatc ttttcgaacg acttcgctga ccgagaagcg gacagcgtgc agcgaacgat
     1381 tctctccgga ggttcaggcg tcatcgcaga gggcaggctc agcccgcacc aggtgaagcg
     1441 cgccgctcgt ggcgctgcca tctcgctcat cgcctattca tgcttgttgg ccgttgcggg
     1501 aaggggttgg acgccggtct acggcctcgc tgcgatcctg ctcatgtggc tctacagctt
     1561 cccgcccgtc cgcctgagct atcgtggcgg tggggagctc cttcaaggaa tcggcatggg
     1621 gatcggtctt ccttcgctcg gttactacct gcaaaccgaa ggtcctttcg ctccgggatg
     1681 ggtgatctgg cccgccgctt tgctcggagt gtgcagcaac gtcctcaccg ccctcccgga
     1741 cgtcgaaaac gacgcgcgag ccggcaagag aacctggccc gtccggctcg gcattcgctc
     1801 ggctcgccgt tttggctccg ccggcatcgc ctttgcggca ttcggtgtgt tcctctggac
     1861 cccaggggtt cctgtgcctg ccaaggcgct gctgtcgatt gcgccgctcc tgcccctgct
     1921 cgtagccgcc cgggacgaag agccattcca tgccgcctgg tggtcgagca tcgcgctcaa
     1981 tctcttgttg gcgctctgga tgctcgcgat gattgtgccg ctctagcggc tcaaaccgag
     2041 acaagctgcc gcctcttccc tcagcgcgtc gaaatcgcgg ctcgagtcgg gtctggcatc
     2101 gaaccgccaa gtctcgaccg gcacctcacc gcgttccgca agcagccggt agagcggaag
     2161 ctcgtgctcg ggttcacggg cgaagggaaa cgggtttcga gcgaccccgt cttcgaggct
     2221 cgggtagagc gcatcatctt catcgatgag tttgagaatg tcacgacccc ggttttcaga
     2281 ttgttggagg aactccatac caaagggcgc cgcctcctgg cgcgaccccg gatcccggcg
     2341 aacccaacgt gcccagtcga acccgtacat gaaatcgtgc acgtatcgaa accgaatcgc
     2401 tgactgttgg ccgtacaggt tcatgaggcc caccgtgacc cgggtgacct ttcgcaagtg
     2461 atccgcgtag tcgggcgagg ccaaagcgtc tcggtgaatc gaggccagga agaagtcgaa
     2521 atcccagaag aggttctcag gaaagcttct gagctgcgcg tctgccacgg caactgccgc
     2581 ggcgccgagg tgcggcgttt cgccagggtc agtgaggccc atgaccaagt cgtcgagccg
     2641 gttcgcagtg ctccgacagg gatcgagggt caagcgtcca tacgcggtta gcccgattcg
     2701 ttcgtcgatc tcggccagtg ttgcaccgat gtgatttgtc gcgtgggcgg tcatgtgagt
     2761 atcgatcgca gccatagaag caccgcaacc aggggagtac tatgccagat cgctcgatga
     2821 agaaagcgct tgtactcggc ctgttctcga aaggcattgg tggcggcgct cttgctgacg
     2881 cgccgcatcg cggcaaagtt ccatgaaacg atcagcacgg cgcttgcaga aacgtagaac
     2941 ggagcccggc tcggtgccgc gatcgatccg gccagccaaa ccccggcgcc tagcacgacc
     3001 agggcttccg tgagcgcgcg cgccgccgca ttgccgcgca tggacgcgaa cgtgcgcttg
     3061 cctccgaccc ggtcgctttc ctcgtcggag agcccgctcg cgaccccact ggccagactc
     3121 aaagccgaga accccaagat ccaaggccat gctgcgagcg ctacggaccc ggcctgcaag
     3181 taggcgttgt atacgggaag tgcgagaccg acaccagcca tctcgagaag ctctccaccg
     3241 cctcggtagt tcaaacggat cggcgggagc gtgtaagcaa cgaacagcag catgcaggcc
     3301 aatccggcct cgaacgcgaa gggtcgcaat agaaagcgct ccgcgcccgc cgcgaccagc
     3361 aaggtcgcag cgccaaagag gacgcccgcg atcgcgatcg cgcgcgagtc gatcaggcgg
     3421 tcaggtatcg tcttcggcga acagccatcc gggaacatgt tccgcttgat cgcgtcgact
     3481 tcgcgatctc cccaatcgtt caacaatacg atgaagccta gaccgaagag cgtgaacgcg
     3541 aatccccatc ccaaagcctc gaggcccagc gctcctttct cgcttgcgcc aagcgcctgc
     3601 ccaaagagag cgggcacgaa tagcttaggc caactggctg gcttcaatac atacgcccat
     3661 cgctctaggc gcgactccgg cggcaggtcg ctggtaaacg aagtcctcat gggtcctcaa
     3721 tcaggttccg gcggagtggg cggatcgctc tcgatgtgct tggcacggac ttcccacttc
     3781 gcgcgcgcca tggc
//
LOCUS       DASRZF010000427         6722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3423865, whole genome shotgun sequence.
ACCESSION   DASRZF010000427 DASRZF010000000
VERSION     DASRZF010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 6722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6722
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3423865"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="VFG22_18790"
     CDS             <1..1041
                     /locus_tag="VFG22_18790"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET6418351.1"
                     /translation="ELNLGRLRPLLPTIVALTGWAVVGTSVLLGLALFVAAPRLGFLE
                     GLEDTTLLAFCGMLGVVLSAQSPAVVMAMLSETRSDGPVSRTMLGTVVIADLVVIVLF
                     SGASAVVRSLMTGEGDAAGTARTIAWELFGSMGSGIVVGILLAYYLRKVRRDASLFVV
                     LLCIIVAEITTRVSLDPLIVMLSAGLVIANFVESDASSLVHDLQAASLPLYLVFFAVA
                     GTTLHLDLLFEFGAIAAILVALRALGFGLGGAIATRQTRSTVEIRRWAWTGLLPQAGL
                     ALALALVVQRSFGDWGKGASALLLGVIAMNELFMPIVLRNALLRSGEAMVRSSVAPAV
                     RLPAPSPPGEQP"
     gene            1076..2860
                     /locus_tag="VFG22_18795"
     CDS             1076..2860
                     /locus_tag="VFG22_18795"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HET6418352.1"
                     /translation="MAQPVSIEAWIACATVLAVLVLLASTRMAPDFVLAGALTMLVAA
                     GILSPSQALSGLSNPGLATVAVLFVVAAGLVDTGAVAAVSNTLLGKVRSVTGAQARMM
                     FPVIAVSAFLNNTPVVAILVPVVQDWCRRQRMSVSKFMMPLSFAAILGGTCSLIGTST
                     NLVVHGLVLAQTDLGPMGLFSIAKVGLPAAIIGAVYVLTMNRRLLPERRPPLTENPNA
                     REYAIEMLVREESPIVGRTIEEAGLRHLPGAFLAEIERGSSVLPAVAPTERLQVGDRL
                     VFVGAVDSVVDLVKLKDLVPAPDELFELDTPRPERKLVEAVISRASPLAGQTIRDGRF
                     RSRYGAVVIAVARDGERLLGKVGDIRLRAGDTLLIEARPSFLEQQRHSRDFLLVSEIQ
                     GASPPRHERALVSAVILSGMVVAAATFADMLLAALVAAGLMVATRCTTVARARASIDW
                     SVLAVIGASFGLGKSLEVTGAASHLAQSWLSLAGEGPWLALAAVYLVTMLFTEFITNN
                     AAAVLVFPIAQATAGALGVSFWPFVVAIMMAGSASFATPIGYQTNLMVYGPGGYRFTD
                     YIRFGLPLNLLLAAVTICLIPLFWPFYP"
     gene            2878..4188
                     /locus_tag="VFG22_18800"
     CDS             2878..4188
                     /locus_tag="VFG22_18800"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6418353.1"
                     /translation="MRLSLADALFYALMVGAGEVYFLADAVRLGATSLEQALVITLPL
                     CVGTLGPLMALGLLGRFRRRKAVVVSAATLQALILFTIVLTDTKGTLSPRVLIGLASA
                     YQMCAQAAGTAWASWYGDLVPARVRGRYFATRNRIAQVATSGSLIVAGLLLQRLEPGS
                     AGTVAPGAGGLGYAIIFGGAGLFRLVSAVLLALSPEPPAQLLSDRRETLSFARSDEAL
                     PVRRILLFAAALQLTVYMASPYFGPFMLEELRLSYTEYMAGSLAVVVLKFASLPSWGR
                     LIDRYGARAAFLLAVVLVALVPLPWLWVRGVAMVIVAQALSGLSWGGHEVSQFSLLLE
                     TSHARSRLHVFAMMSVFTGVAQLTGSLLGGWLLMSVDRNFLVVFVVSAACRLGVALAA
                     PAIIPASIGTPGIGRRRLFLRLLGFRASGGMESRPMPVDDIEGS"
     gene            complement(4185..5534)
                     /locus_tag="VFG22_18805"
     CDS             complement(4185..5534)
                     /locus_tag="VFG22_18805"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HET6418354.1"
                     /translation="MAVPAWKELATQGEGPTAAFVDATSTDFSKTRVLHGARRDSGDG
                     SRKAGEPVTREDTVELRVPLERVLRAGHPWVFRRALRKPPRSAGRVVAVHGRDGRFIG
                     RGISDDGAIAVRMFTTRDEPVDGRLFAERLAEAFKLREQILPPETNAYRLVHGEGDRL
                     PGFVVDLYGDYAVLRFDSPALHSWLPTWLEALVPMFGRLGVRGAVARLPKDKDFGVQQ
                     VWGEPPPELVTVVERGMKLVASLWHGQKTGLFLDHRESRWRIRELSEGCRVLNLYGYT
                     GGFSVAAGLGGAREVVTVDSARPALELATQSWNANALKGQRHRVVVSDVPRYMHALRD
                     DERFDIVISDPPSFAPNQRAVPRAIRAYQNLHQSCMQHVADDGLYLAASCSSHISLDV
                     FETSLRDAARATNRSATILETWGAAPDHPVLRVFPEGQYLKVCLLRIGDRRGTDQRA"
     gene            5766..6017
                     /locus_tag="VFG22_18810"
     CDS             5766..6017
                     /locus_tag="VFG22_18810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418355.1"
                     /translation="MRANVRSSVPAKDQSLVPPPDVDSADIDETEPKTKRAERRTGGW
                     AEDWTDSSKQHQVSALWPLMWLTLPLVLLVLYGLFSGHR"
     gene            complement(6051..6434)
                     /locus_tag="VFG22_18815"
     CDS             complement(6051..6434)
                     /locus_tag="VFG22_18815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418356.1"
                     /translation="MGKGVAILCLLAGGYVSVAALGVLPPEYAVIHAPRGVVLGGGAL
                     LVVIGFMSLARDHRMSDAIASMLLLALAAGAGWLTFYAPEGTLSRYVPFIPSSVNEAL
                     ARLLFGFGAAASVGMAIWGVRRIFR"
     gene            complement(6452..>6722)
                     /locus_tag="VFG22_18820"
     CDS             complement(6452..>6722)
                     /locus_tag="VFG22_18820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418357.1"
                     /translation="YETGNLGVGAIAALAWIKLGRVTDPRLGARATVRDGRRLAKQAA
                     WFLEVPWHEWLARPLDDVRRDLGVSGPVQYRPVRSSEVDLSLMHS"
BASE COUNT         1068 a         2252 c         2067 g         1335 t
ORIGIN      
        1 gagctcaatc tcggtcgctt gcgcccactg ctgccgacga tcgtcgccct gacaggttgg
       61 gcagtcgtgg gcacgagcgt tctcttggga ctggctttgt tcgttgcggc gccgagactc
      121 gggtttctag aggggctgga ggacactacc ttgctcgcat tctgcggaat gcttggcgtc
      181 gtgctcagtg ctcaatcccc agcagtggtg atggccatgc tgagcgaaac ccgctcggat
      241 ggccctgtca gcaggaccat gctcggaacg gtcgtcatcg ccgatctagt ggtgatcgtc
      301 ctcttcagcg gtgcttcagc ggtagtccgc tcgctgatga ccggcgaagg cgatgcggcc
      361 gggaccgcac gcacgatcgc ttgggagctc ttcggatcga tgggatccgg aatcgtcgtt
      421 ggtatccttc tggcctacta cttgcgcaag gtccggcgag acgcctcgct cttcgtggtt
      481 ctgctttgta tcatcgttgc ggagatcacg acgagagtga gcttggatcc cctgatcgtg
      541 atgctgagcg ccggactcgt catcgccaac ttcgtcgaat cggacgcttc ctcgctcgtc
      601 catgacctcc aggccgcctc ccttccgctt tatctcgtat tctttgcagt tgcggggacc
      661 acgcttcacc tcgacctctt gttcgagttt ggagcgatcg ctgcgatcct ggtagccctc
      721 agggccctgg ggtttggcct cggcggagcg atcgccaccc gacaaacgcg ctctacggtg
      781 gagatccgcc gttgggcttg gacgggccta ctgccccaag ccggcctcgc cctggcgctg
      841 gcactggtcg tgcagcgctc gttcggtgac tggggcaagg gcgccagcgc gcttcttctc
      901 ggcgtcatcg ccatgaacga gcttttcatg cccatcgttc tgcgcaacgc cctactccgg
      961 tcgggcgaag ccatggttcg aagctccgtc gcgccggccg ttcgccttcc cgcccccagt
     1021 ccgccgggcg agcagcccta gcgcactcaa ttggctagtc gtcccgaact tggctatggc
     1081 tcagcccgtg tccatagaag cctggatcgc ttgcgccacg gtgttggcgg ttctcgtgtt
     1141 gctggcaagt acccgcatgg cgcccgactt cgtcttggcc ggcgcgctga ccatgctcgt
     1201 ggctgccggc atcctctcgc caagtcaggc cttgtcgggc ttgtcaaacc ctggcttggc
     1261 caccgtggcg gtcctcttcg tcgtggccgc cggcttggtc gacaccggtg cggttgccgc
     1321 ggtgagcaac acactgctcg gaaaggtccg ttcggtcacg ggcgctcagg ctcgaatgat
     1381 gttccccgtc atcgcggtga gcgcctttct gaacaataca cccgtcgtcg cgattctggt
     1441 acccgtcgtg caggactggt gccggcgtca acgcatgtcg gtctcgaaat tcatgatgcc
     1501 cttgagcttc gccgccatcc tcggaggcac gtgctcgctc attggaacca gcaccaatct
     1561 ggtcgtccac ggcctcgtgc tcgcgcaaac cgatctcggt ccgatgggct tgttctcgat
     1621 cgccaaggtt ggattgcccg cggcgatcat cggcgccgtc tacgtgctga cgatgaaccg
     1681 gcggctattg cccgaacggc gcccaccgct caccgagaac cccaacgccc gcgaatacgc
     1741 cattgaaatg ctggtccggg aagagagccc gatcgttgga cgcaccatcg aagaagcagg
     1801 gctccgtcac ctacccggcg cgttcctcgc cgagatcgag cgcgggagct cggtgctccc
     1861 ggcagttgct ccaacggaac gcctacaagt tggggatcga ctggtcttcg tgggtgcggt
     1921 cgactccgtg gtcgatctcg tgaagctcaa ggacctggtt ccggcgcccg atgagttgtt
     1981 cgagctcgac acgccacgtc ccgagcgcaa gttggtggaa gcggtcatct cgagagcctc
     2041 tccattggca gggcagacaa ttcgggacgg ccgctttcgc tcgcgctacg gcgctgtcgt
     2101 catcgccgtg gcccgcgacg gcgaacgcct gctcggtaag gtgggcgaca ttcgactgcg
     2161 cgctggcgac accttgctca tcgaagcgcg cccttcgttc ctcgagcaac aacggcattc
     2221 gcgagacttc ttgttggtaa gcgagatcca aggcgcttct ccgccacgcc acgagcgcgc
     2281 actcgtttcc gccgtcatct tgtcaggaat ggtcgttgcc gccgcgacct ttgccgacat
     2341 gttgctcgca gcgctggtcg ctgcagggct gatggtggct acgcgctgca ccaccgtggc
     2401 gcgcgcacgc gcgagcatcg attggtccgt cctcgccgtg atcggcgcgt catttggtct
     2461 cggcaaatcg ctcgaggtga ccggggcagc atcgcacctg gcccagagct ggctttcgct
     2521 ggcgggcgag ggtccgtggc tcgcgcttgc ggccgtgtat ctcgtcacga tgctcttcac
     2581 ggagttcatt accaacaacg ccgccgccgt cttggtgttt cccatcgctc aggcgaccgc
     2641 cggagcattg ggcgtgagct tctggccctt cgtcgtggcg atcatgatgg cgggctcggc
     2701 aagcttcgcg actccgattg gataccaaac caatctcatg gtgtacggtc cgggcggcta
     2761 ccggttcacc gactacatcc gcttcgggct cccgctcaat ctcctcttgg ctgcggtgac
     2821 catctgcctc attcccctct tctggccgtt ctacccgtga gttacacgcg tcgcgccatg
     2881 cggctttcgc ttgcggacgc cctcttctac gccctgatgg tgggcgccgg cgaggtctat
     2941 ttcctcgcag atgccgtacg gctcggtgca acctcgctcg agcaagcact ggtgatcacc
     3001 ttgccgctct gcgtgggaac gctcgggccg ctgatggccc tgggcctcct cggccgtttt
     3061 cgtcgccgca aagcggtcgt ggtcagcgca gcgaccctgc aggctctgat cctctttacg
     3121 atcgtgctca ccgatacgaa ggggactctc tcgcctcgtg ttctcatcgg cttggcatcg
     3181 gcgtaccaga tgtgtgcgca ggccgccggc accgcctggg cgtcctggta cggagatctc
     3241 gttccggcgc gagtgcgggg ccgctacttc gcaacacgga accgcatcgc ccaagtggcg
     3301 acatcgggct cgttgatcgt tgccggcttg cttctacaac gtctcgagcc tggctccgcg
     3361 ggcaccgtcg ctcccggcgc cggcgggttg ggctatgcga tcatcttcgg cggtgccggc
     3421 ctgtttcgcc tggtctcggc cgtcttgctc gccctctccc cagagccgcc tgcgcagctg
     3481 ctgagcgatc gacgggagac tctctcgttt gcgcgctcgg atgaagccct ccctgtccgt
     3541 cgtattcttc tcttcgccgc cgcgttgcag ctcaccgtgt acatggcctc tccctacttc
     3601 gggccattca tgctcgaaga gctccgcctt tcctacaccg aatacatggc cggaagtctc
     3661 gcggtcgtgg tcctgaagtt cgcttcactc ccatcctggg gtcgcctcat cgatcgctac
     3721 ggcgcccgcg ccgcattcct gttggcggtc gtcctcgtcg cgctcgtgcc cctgccatgg
     3781 ctctgggttc ggggcgtggc catggtgatc gtcgcccaag ctttgtccgg cctttcctgg
     3841 ggcggacacg aggtgtctca gttctcgctg ctgctcgaga ccagccacgc gcggtcgcgc
     3901 cttcatgtct tcgcgatgat gagcgtgttc accggcgtcg cacagctcac cggaagcctc
     3961 ttgggcggct ggttgctgat gagcgtcgac cgaaacttcc tcgtcgtctt cgtggtgagc
     4021 gcggcctgcc gcctcggcgt agccctcgcg gcgccggcga tcattccggc gagcatcgga
     4081 acgccaggca tcggccgccg tcgccttttc ctcagactcc tcggcttccg cgccagcgga
     4141 ggcatggaaa gccgcccaat gccggtcgat gacatcgagg gaagctaagc tcgctggtcg
     4201 gtgccccgtc gatctccaat tcgcagcagg cacaccttga gatactgacc ttccggaaaa
     4261 acacgaagca ccggatggtc aggcgcagca ccccaggttt ccaagatcgt tgcggagcgg
     4321 tttgtcgccc gcgccgcatc acgaagcgat gtctcgaaaa cgtcgaggct gatgtggctc
     4381 gaacaggaag cggcgagata gagcccgtca tccgcgacgt gctgcatgca gctctgatga
     4441 aggttttggt atgcgcggat cgctctgggc accgcccgct ggttcggggc gaagctcggt
     4501 ggatccgaga tgacgatgtc gaaacgctcg tcgtccctca aggcatgcat gtaccgggga
     4561 acatctgaaa cgacgacgcg gtgtcgctgc cccttgaggg cattcgcatt ccagctttgc
     4621 gtcgcgagct ctagggcggg gcgcgccgag tcgaccgtca cgacctcgcg ggctccaccg
     4681 aggccagccg ccacggagaa cccgcccgtg tacccgtaga ggttcagcac ccggcaaccc
     4741 tcgctcagtt cgcggatgcg ccagcgcgac tcgcgatggt ccagaaagag ccccgttttc
     4801 tgcccgtgcc agaggctcgc cacgagcttc atgccacgct ccaccaccgt caccagctcg
     4861 ggtggaggtt ccccccagac ctgctgcaca ccgaagtctt tgtccttcgg cagccgagcc
     4921 acggcgcccc gcacacccaa tcgcccgaac atcggcacca aggcctcgag ccaggtcggc
     4981 aaccaggaat ggagcgccgg gctgtcgaaa cgcagcacgg catagtcccc gtacaagtcg
     5041 acgacgaacc ccggaaggcg gtcgccctcc ccgtgcacca gccgatacgc gttcgtttct
     5101 ggaggcagga tctgttcacg gagcttgaaa gcctccgcga gtcgctccgc gaataggcgc
     5161 ccatccaccg gctcgtcacg cgtcgtgaac atgcgcacgg cgatcgcgcc atcgtcggaa
     5221 ataccgcggc cgatgaagcg gccatcacgg ccatgcacgg caacgacgcg gccggcgctc
     5281 cgaggcggct tgcgcaaggc tctccggaag acccatggat gacccgcgcg caacacgcgc
     5341 tccaaaggaa ccctgagctc caccgtgtct tctcgcgtca caggctcgcc ggcctttctc
     5401 gacccatcgc cactgtctcg tcgagcacca tgtagcacac gggtcttgct gaagtcggtg
     5461 gaggtcgcgt cgacaaacgc tgccgttgga ccctccccct gggttgccag ctctttccaa
     5521 gccggcaccg ccatcggcgg tcgttgtggt tgataaccca gcgccctggc gatccagctt
     5581 ggcaagtgcc tggcgcggat ccagaatcag ggatgcggtt ggcattccag ctggtcgttg
     5641 tggcggtgct cggctcgggg ctgagcgacg accctcccga acgacctcgt tctttgttct
     5701 gagtcgaagt tcctggtttg ttcgatcaag cgccttccga gtcggacggc gtggaggtaa
     5761 gtgtgatgcg cgcgaacgtt agaagcagtg tccccgcgaa ggatcagtcg ctggttcctc
     5821 cgccggacgt ggattcagcg gacatcgacg agacggaacc gaaaacaaaa cgggccgagc
     5881 ggcgtaccgg gggctgggcc gaagattgga ctgactccag caagcagcat caggtgagcg
     5941 cactctggcc gctgatgtgg ctgacgctgc cattggtact gctggttctg tacgggctct
     6001 tctccggaca tcggtgaatc gcaggggtcc gctcaatccc gctctgcgct tcagcgaaag
     6061 atccggcgga cgccccagat cgccataccc acggatgccg cggccccgaa tccgaacaac
     6121 aagcgcgcta gcgcctcgtt gaccgaggat gggatgaacg gaacatagcg gctgagagtg
     6181 ccttcgggcg catagaacgt gagccatccg gctcctgctg ccagcgcaag caacaacatg
     6241 gacgcgatcg cgtcgctcat tcgatggtcg cgggcaagcg acatgaagcc gatcaccacg
     6301 agcagcgcgc cacctccgag aaccacgcca cgcggcgcgt ggatgacggc atactccggg
     6361 ggcaacacgc cgagagccgc gacggagaca tatccccccg cgagcaagca tagaatcgcg
     6421 actcccttcc ccactcccgc agcttagcgg atcaggagtg catcagcgaa agatcgacct
     6481 cgctggagcg gaccgggcga tactgaaccg gtccgctcac ccctagatcc cgacgcacgt
     6541 cgtcgagggg ccgagcgagc cattcgtgcc agggcacctc gagaaaccag gccgcctgct
     6601 tcgccaaacg ccgtccgtct cgaaccgttg ctcgggcgcc cagtctcgga tcggtgaccc
     6661 gacctagctt gatccaagcg agcgctgcga tcgcgcccac accgaggttg cctgtctcgt
     6721 ag
//
LOCUS       DASRZF010000428         6926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_5136565, whole genome shotgun sequence.
ACCESSION   DASRZF010000428 DASRZF010000000
VERSION     DASRZF010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 6926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6926
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5136565"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="VFG22_18825"
     CDS             <1..733
                     /locus_tag="VFG22_18825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418358.1"
                     /translation="PALMAVIYMWSVGVAHKRDPRYILPIGFAAGILVKLAYNLTYVH
                     LWHQGSWSFALSMLMTTFFFCVLVGDSYARLDARGTAKKALFIGYALVLIFSMGRQIL
                     GSAYVDHNRSYDFWADRVAIQEALDRLAPGAKLLELDDGIISFSIESPSIHAFGFAAD
                     KDSAMALREGRMLAHAHERGYDIFTSNGYVAIDPQMDAESIRTRLRESGAVQDELRSE
                     LDSFDFELLWVHEPTRTGFIRFKPL"
     gene            complement(806..988)
                     /locus_tag="VFG22_18830"
     CDS             complement(806..988)
                     /locus_tag="VFG22_18830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418359.1"
                     /translation="MSSELGLEDRACTPLVSAATGASGLFREQLHETHDTTPSNPIVA
                     NTLLLIPDPRAAALTQ"
     gene            1014..2510
                     /locus_tag="VFG22_18835"
     CDS             1014..2510
                     /locus_tag="VFG22_18835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418360.1"
                     /translation="MAGVVDYSRDIPFVDLFKSSRPWISGSDEKWDDKRPIDLDEDGW
                     VRSLMPGQVARTLMLTEGGHPGGEFIVVYDGEGEIAYGNELLQNQSRPGRQVIRVKKD
                     GAVRFAISKTSPSNPIRNIRVFPPGGSCEEDDALWCDGSHPCPTGRCLPFEDTHPDRI
                     FHPKFLQSTSNYSVLRFMDWMRTNDSTISTWSERPKLTHATWNRRGVPVEVMISLAVR
                     LQAEPWVNIPHLADDDYVRQFATLVRDTLPEEFRVWLEYSNEVWNPQFKQHHEVARCP
                     VGKSENGEFGAALVCQAERSDAIFQIWHQIFGESASRVVRVVATQAANAWTSKKVLTH
                     RDLYERVDALAIAPYFGIAVNRKTRADYTAMPVDELLERTEAEVMPKAIKRMQDQAEM
                     AARFGVTLVAYEGGQHFSAAAGAENDENINALFDAINRHPKMSDLYLRYLTAWREAGG
                     KLFVHYNNCGGYTKWGRWGLREYVDQPRAEAPKLDGVMRFIEQNPRWW"
     gene            2581..4020
                     /locus_tag="VFG22_18840"
     CDS             2581..4020
                     /locus_tag="VFG22_18840"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HET6418361.1"
                     /translation="MVQSYDVVCIGSGFASSFFLQEYLRHAPATARILVLERGPALSH
                     AQLIARRDELTYESFQHTFVNETPAKPWIFTLAHGGSSNCWGGNTPRMIPNDFRLRSR
                     YGVGFDWPISYDDLEPYYCEVEETFGVAGPDDTVYPRSKPYPCPPHQMSDPDRAMAAA
                     YPGLFFSLPVAKPTRSRLPARPQCCSSLRCNLCPVDSKFTILNGMRPEYADPRVELRA
                     NATAERIEHSAGIATGVHFTAEGAKLFAKAELVVLGANGIFNPHILLRSGLDGEWVGR
                     GLCEQVSILCTVELDGMDGFQGSTTQTALGYMLYDGPHRRDRAAAMFESYNIPNLRNL
                     RGRWRQRYVIKFVLEDLPQERNRVRLDTDDSSRPVTEYHGHSEYTHRGLEAVRASLQE
                     LLKPLPVESFRMHDEIEQTDYHIQCTTMMGRNPKDSVVDANCRHHRVRNLVVVGSSVF
                     PSAAPANPTLTLSALALRSARSLFGGQAT"
     gene            4017..4409
                     /locus_tag="VFG22_18845"
     CDS             4017..4409
                     /locus_tag="VFG22_18845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418362.1"
                     /translation="MSTSHRVTRRVFVTSLLGAAALTAGAGVWHWDSLRREWIVRKLI
                     DHFDYLDIDPAGVSRFVDTFERHQYPIRRNPLKLLFRPLPDEIYMNYLSSTDFFINGA
                     DESRPVRFVALYDPYVSPCYNPFRRVQA"
     gene            complement(4428..6170)
                     /locus_tag="VFG22_18850"
     CDS             complement(4428..6170)
                     /locus_tag="VFG22_18850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET6418363.1"
                     /translation="MECPRVQSARASLDEPSQSPASLGAGGKKWIDRTGPYLVVLAFG
                     WMVAAALVNPVAEIPLNDDWAYSHVVRTLFDEGRLVFTDWQSMPLLTQVLWGALFCLP
                     FGFSFTALRVSTLVAAYLGAVALFGLLRELGATRRQAFFAAACLLCSPLYFSLSFTFM
                     TDVPFVAACLGAVWLWVGGIKRGSTIWTVVAVGLSICATLNRQLGLALPLAFAAGELV
                     RLGGTRRVLLRMVLPVACTAAALLLYQHWLATGPGTPELYHQKEDALRAAISELLLLR
                     GWRLPLERTLGALLLLGGMLVPLLWLLPLPGLQKGPARRAAIAAAAAMALLAAVLGLP
                     ESTHGDIMVGAGLGVRTSLGTHWQPPDWLGKAVLVAACLPATAVAALVARALLDVALK
                     LWPARCPAPKAVHKLRNGLPSLAIEPGYAAIFVALLALLHAPTSIVHAAYFDRYTVVL
                     IPWALVLWWASSASKQGVTVKPRAGLLLLALFLIADVAATHDYLAWQRARWSAVEYLS
                     QHGVQPAQIRAGFEVDQYGPWAEPLAARADQVSPFYLVAISPIPGHRTLHAVPVNRWL
                     PFTASQVLVLRPDE"
     gene            complement(6274..>6926)
                     /locus_tag="VFG22_18855"
     CDS             complement(6274..>6926)
                     /locus_tag="VFG22_18855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418364.1"
                     /translation="YTRYHYSPADLPSMFRTARWPLLVLGGWAVAGLIGGGPTIIRLL
                     YDSRYWEAGWMTQIVAAGFWFGIVLGGTYAAVVLAVGRSDWTASMSFSKVLGMVAFIP
                     LGYWLGGFPGAVGGLAMSELIRYCVAVYGAAALGFDEHREDLAISVRVGISALAGWSA
                     VAGLTAFGISHVVIHALTVFVVVTAFWASPFIMLISRTRREKSLFGSDANLSKESV"
BASE COUNT         1414 a         2175 c         2098 g         1239 t
ORIGIN      
        1 ccccgccctg atggccgtga tttacatgtg gtcggtcggc gtcgcacaca aacgcgatcc
       61 tcgctacata ctgcccatcg ggttcgccgc gggcattctg gtcaagctcg cttacaacct
      121 cacctatgtg catctttggc atcagggaag ctggtccttc gcgctttcga tgctcatgac
      181 gactttcttc ttctgcgtgc tagtcggaga ctcgtacgca cggctcgatg cgcgaggcac
      241 tgcgaagaag gctctcttca tcggttacgc cctcgtcttg atcttctcga tgggccgaca
      301 gatcctcggt tcggcctacg tagatcacaa ccgttcatac gatttttggg cggatcgagt
      361 ggccatccag gaggcgctgg accgcctcgc tcctggggcc aagctgctcg agctcgacga
      421 cgggatcatc tcgttttcga tcgagtcgcc atcgattcac gcgttcgggt ttgcagcgga
      481 caaagactct gccatggcgc tacgtgaagg acgtatgctc gcgcacgccc acgaacgtgg
      541 ttacgacatc ttcacttcga acggctacgt ggctatcgat ccgcagatgg acgccgagtc
      601 gattcgaacg cgcctccgag aaagtggcgc cgtgcaggat gagctcaggt ccgagctcga
      661 ctcattcgac ttcgagcttc tgtgggtgca cgagccgacg cgcaccgggt tcattcgctt
      721 caagccgctc tagggctccg ttcgctgcaa gcttctcgag cacattgcgt gaattgctgc
      781 atagatgctt tcatgtcagc ttcactcatt gagtgagcgc agcagcgcgg gggtctggga
      841 tgagaagaag cgtgtttgcg acaatcggat tgctcggggt cgtatcatgc gtctcgtgca
      901 actgttcccg gaacaaaccg cttgctccgg ttgctgcgga aacgagcggt gtgcacgcgc
      961 ggtcttcgag gccgagctcc gagctcaatc gtacctcatc gataggatcc aacttggccg
     1021 gggtggtgga ctacagccgg gacatcccgt tcgtggatct gttcaagtca tcgcgcccgt
     1081 ggattagcgg tagcgacgag aaatgggacg acaagcgccc gatcgatctc gacgaagacg
     1141 gctgggtccg ttccttgatg ccaggccagg tggctaggac gctcatgttg acggagggcg
     1201 gtcaccccgg gggcgagttc atcgtcgtct acgacggaga gggcgagatc gcctacggca
     1261 acgagctgct gcagaaccaa tcgcgtcccg gccggcaggt gattcgcgtc aagaaggacg
     1321 gcgccgtccg tttcgcgatc tccaagacca gtccgagcaa tccgatccgc aacattcgtg
     1381 tgtttccgcc tgggggatct tgcgaggaag acgacgcgct ctggtgcgat ggctcccatc
     1441 cctgcccgac tggtcgctgt cttcctttcg aggatacgca tccggaccgg atctttcatc
     1501 caaaattcct gcagtctacg agcaactatt cggtcctgcg cttcatggat tggatgcgga
     1561 ccaacgattc gacaatcagt acttggtcgg agcgtcccaa gctgacgcac gcgacctgga
     1621 atcggcgagg cgtgcccgtc gaggtgatga tttcgctcgc agtgcggctg caggcagagc
     1681 cttgggtcaa catccctcat ttggcagacg acgattacgt tcgtcagttc gcgactctgg
     1741 tccgcgacac tctgcctgaa gagtttcgcg tctggttgga gtactccaac gaggtctgga
     1801 acccgcagtt caaacagcac cacgaagtgg cgcggtgccc agtggggaag agcgagaacg
     1861 gtgaattcgg ggccgcactc gtgtgccaag cggagcgaag cgacgcgatt ttccaaattt
     1921 ggcatcaaat cttcggggaa tccgcttcgc gggtcgtccg cgtcgtcgcg actcaggccg
     1981 ccaacgcttg gacgagcaag aaggtactaa cgcaccgcga cctgtacgag cgggtggacg
     2041 cgctagcgat agccccgtac ttcggcatcg cggtgaaccg aaagacccga gcggactaca
     2101 ccgccatgcc ggtggatgag ctcctcgagc gcaccgaagc cgaggtcatg ccaaaggcga
     2161 tcaaacggat gcaggaccag gccgagatgg ccgcgcgctt cggcgtgacc ctggtcgcgt
     2221 acgagggagg gcagcacttc tcagcagcgg ctggcgctga gaatgacgag aacatcaacg
     2281 ccctttttga tgcaatcaat cgccacccca agatgagtga tctctatctg cgctacctga
     2341 ccgcgtggcg cgaagcgggc gggaagctgt ttgtccatta caacaactgt ggtgggtaca
     2401 cgaagtgggg gcgctgggga ctgcgggagt acgtcgatca acctcgcgcg gaggcgccaa
     2461 agttggacgg cgtgatgcgg ttcattgagc agaacccgcg ttggtggtga ctgagacgcc
     2521 ctcgcccaag ggattcatgg catttgaagt catgaacagc ctctgctact cgctgcaagt
     2581 atggtgcaga gctacgatgt cgtttgcatt ggcagcgggt tcgcgtcgtc cttctttcta
     2641 caggaatatt tgcgccacgc accggcgacg gcacgaattc tggtgctcga acgcggtcca
     2701 gcgctttcgc acgcgcaact catcgcccgt cgcgacgagc taacgtacga atctttccag
     2761 cacacgttcg tcaacgagac ccccgcaaag ccatggatct tcacgctagc gcatgggggg
     2821 agctccaact gctggggtgg caacactcct cggatgattc cgaacgattt tcgactgcgc
     2881 tcgcgctatg gcgtcggctt cgactggccc atcagctacg acgacctcga accgtactac
     2941 tgcgaggtgg aagagacctt cggagtcgcc ggcccggacg acaccgtgta ccctcgcagc
     3001 aagccctacc cgtgtccgcc gcaccagatg agcgatccag accgtgcaat ggcggccgcg
     3061 tatcccgggc tgttcttctc tttgcctgtc gcgaaaccca ctcgttccag gctgcccgcg
     3121 cgcccgcagt gttgttcgtc actgcgctgc aacctctgtc ccgtcgattc caagttcacc
     3181 attctgaacg ggatgcggcc cgaatacgcg gatccacgcg tagagctgcg ggccaacgcg
     3241 accgccgagc gaatcgaaca ttctgcaggc attgcaacgg gggtgcattt caccgcagaa
     3301 ggcgcgaagc tgttcgcaaa ggccgagctg gtggtactcg gcgccaatgg tatcttcaat
     3361 ccacacattc ttctgcgctc cgggctagac ggcgagtggg ttgggcgcgg actgtgcgag
     3421 caggtcagca tcttgtgcac ggtcgagctg gacggcatgg acggctttca gggaagcacc
     3481 acccagaccg cactcggata catgctgtat gacgggccgc accgacgaga ccgagccgcc
     3541 gccatgttcg agagctacaa catccccaac ctacgcaatc tgcgtggccg ctggcgccag
     3601 agatacgtga tcaagttcgt gctcgaggac ctgccccagg agcgcaaccg tgtgcgtctc
     3661 gacaccgacg actcgagccg acccgtaact gagtaccacg gccattccga gtacacgcat
     3721 cgtggcttgg aagccgttcg cgcctcactg caagagctgc tcaagccgct gccagtcgag
     3781 tcgttcagga tgcacgacga gatcgaacaa accgattatc atatccaatg cacgaccatg
     3841 atgggccgta accccaaaga ctccgtagtt gacgcaaact gccgtcacca ccgcgttcgg
     3901 aacctcgtcg tggtgggctc gagcgttttt ccgtccgcag caccggccaa ccccacgctg
     3961 acgctgtccg ccttggcgtt gcgctccgcg aggagcctat tcggaggcca ggcgacatga
     4021 gcacgtcgca tcgagtcact cggagggtct ttgtcacgtc gctgctaggc gccgccgcac
     4081 tcaccgcggg cgcaggcgtt tggcactggg actcccttcg acgcgaatgg atcgtgcgga
     4141 agctgatcga ccatttcgac tatctcgaca tcgatcccgc cggggtgagc cggtttgtcg
     4201 ataccttcga gcgacaccaa taccccattc ggcgcaaccc gctcaagctg ctgttccgcc
     4261 cgctccccga cgagatctac atgaactatc tgagctcgac ggacttcttc atcaacggcg
     4321 ccgacgagtc gcgccctgtc cggttcgtcg ccctctacga cccgtacgtc agcccttgct
     4381 acaacccgtt tcggcgtgtt caggcctagc cccccctttt ttgctcctca ctcgtcgggc
     4441 cgcaggacca gcacttgcga cgccgtgaac gggagccagc gattcactgg cacggcgtgc
     4501 aatgtacggt gtcctgggat cggagagatc gccacgaggt agaacgggga gacttgatcc
     4561 gcgcgggcgg ccagcggttc ggcccaggga ccgtattggt ctacttcgaa gcctgcacga
     4621 atctgtgcgg gctgcacacc atgttgcgac aagtactcga ccgcgctcca ccgtgcacgc
     4681 tgccaagcca gatagtcatg ggtggcagcc acgtccgcga tcagaaacag cgcgagtagc
     4741 aacagtccgg cccggggttt cacggtcact ccttgtttgc tcgccgatga cgcccaccac
     4801 aggaccaagg cccagggaat cagcaccacc gtgtagcggt cgaagtaagc cgcatgcacg
     4861 atcgaggtcg gagcgtgcaa cagcgcgagc agagcgacga aaattgcagc gtatccgggc
     4921 tcgatggcca aagatggcag tccgttccgc agcttgtgaa cagctttagg cgccgggcaa
     4981 cgggcgggcc agagtttgag cgcaacgtcg agcagagcac gcgcaacgag cgccgctact
     5041 gctgtcgcgg gcaggcacgc agcgaccaat accgctttgc ccagccagtc ggggggctgc
     5101 caatgggtac caaggctggt gcgaacgccc agcccagcgc cgaccatgat gtccccatgg
     5161 gtcgactcgg gcagtccaag tacggccgcc aggagagcca tggccgctgc cgccgcgatc
     5221 gcggctcgac gtgccggccc tttctgtaat cccggcagcg gcaacaacca gagcaagggg
     5281 accaacatgc cgccaagcag caggagagcg cccaacgttc gctcgagcgg caggcgccat
     5341 ccgcggagca acagcaactc cgatatcgca gctcttaggg cgtcttcctt ctggtggtag
     5401 agctcaggcg tcccggggcc ggtcgccaac cagtgctggt acagcagcaa agccgctgcc
     5461 gtgcaggcaa cgggaaggac catccgcagc aggacgcgtc gcgtgccccc gagccgcacc
     5521 aactccccgg cagcaaaggc caacgggaga gccaggccta gctggcgatt cagcgtcgcg
     5581 cagatgctga gcccgacagc cacaactgtc caaatcgtgc tgccgcgctt aatgcccccc
     5641 acccagagcc acacggcgcc caggcacgcc gccacgaaag gcacgtcggt catgaatgtg
     5701 aacgagaggg agaagtacag cggcgagcaa agcaaacagg ccgctgcgaa gaacgcctga
     5761 cgccgggttg cgccgagctc gcggagcagg ccgaacagtg cgactgcgcc gagataggcc
     5821 gcgacgaggg tggataccct cagggccgtg aatgagaagc caaagggcaa acagaagagc
     5881 gcgccccaga gcacctgggt aagcagcggc atgctctgcc aatcggtgaa taccagtcgc
     5941 ccctcgtcga agagcgtccg tacgacgtgg gagtaggccc agtcgtcgtt gagcggaatt
     6001 tccgcaaccg ggttgacgag ggccgccgct accatccagc cgaacgccaa gacgaccagg
     6061 tatggtcccg tgcggtcgat ccacttcttt ccacctgcgc ccaaggaggc cggagactgc
     6121 gacggttcgt ccaaagacgc tcgtgcagac tgcactcttg ggcattccat gcggcggagc
     6181 ctagtcccag ctcgatccga ggcaacatcg gggtgaggcg gatccccaag catgtgacgg
     6241 ggaagcgggg caacttgccg agtgtcctcg cgttcagacg ctctctttcg agagatttgc
     6301 gtcgctccca aacaacgact tttcccgacg ggttctagaa atgagcatga tgaagggcga
     6361 cgcccagaac gcggtgacga ccacgaagac ggtcaatgcg tggatgacga catggctgat
     6421 tccaaacgcc gtcaggccag ccaccgcgga ccagccggcg agcgccgaga tcccaacccg
     6481 aacgctgatc gcgagatctt cgcggtgctc atcgaacccc agtgccgcgg ccccatacac
     6541 ggcaacgcaa taccggatga gctcggacat cgccaatccg cccacggcgc caggaaaccc
     6601 cccgagccag tagccaagcg gaatgaacgc gaccatcccc aatactttcg aaaaagacat
     6661 ggaagccgtc caatcgctgc ggcccacagc gagtacgacc gctgcatagg tgccgccgag
     6721 aacgattcca aaccaaaaac cggcggcaac gatctgggtc atccatcccg cctcccaata
     6781 tcgcgagtcg tacagaagcc tgatgatcgt ggggcctccg cctatcaagc cggcgacagc
     6841 ccatccgccc aagaccagca gaggccagcg ggccgtgcgg aacatgctgg gcagatcggc
     6901 gggagaataa tgatagcggg tataaa
//
LOCUS       DASRZF010000429         8082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3429222, whole genome shotgun sequence.
ACCESSION   DASRZF010000429 DASRZF010000000
VERSION     DASRZF010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 8082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8082
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3429222"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="VFG22_18860"
     CDS             <1..1077
                     /locus_tag="VFG22_18860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418365.1"
                     /translation="LAGLRSRVFLLHNAHEEADVLFHTRWAMSYLRGPLTRKEIRRLS
                     PPPSDQATSQSDARKAPPSIPAASRSVRPVVAAGIDEAFSSDEPGTYRPYLAASVSLH
                     YVRASHGLDAWEERLLLLPLDEESPWESLALLEPDALDLEHPAPTEAEFVEPRPGSVG
                     KTIFRSYGKQIKSRLYQDRPRVLWSCKPLKLKSTPGETKDQFAARAQLTKREHRDAAL
                     EKLSAELAKKLKKIDAGIRRAEDRVEREKGQYDQEKLQTGISMGATVLGAILGSRNMG
                     RATTAARGAGRVAREREDVRRAEDELQDLLGSRRELELEAKEEVRALRAAMDLESVEL
                     EEVLVRPRKSDISVSDLRLAWRLD"
     gene            complement(1052..1543)
                     /locus_tag="VFG22_18865"
     CDS             complement(1052..1543)
                     /locus_tag="VFG22_18865"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6418366.1"
                     /translation="MTTRPFKVLGIQQVAVGAADKSALRRLWVDILGLTPHGNFKSEK
                     ENVDEDIVTMGSGPFTVEVDLMQPIDPEKKPRVDTPPLNHIGLWVDDIDAAVKWLGDR
                     GVRFTPGGVRPGAAGHRVCFIHPKGNESAPISGEGVLIELVEAPQDVREAFEKISRAA
                     RPA"
     gene            1615..2577
                     /gene="trpS"
                     /locus_tag="VFG22_18870"
     CDS             1615..2577
                     /gene="trpS"
                     /locus_tag="VFG22_18870"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HET6418367.1"
                     /translation="MKTSLTGIKPTHLPHLGNYLGAIRPALSLVDRYRTLYFIADYHA
                     VTTVRDADALRGYIRDVAATWLACGLDPERTLLYRQSDVPEVFELTWVLACLLATGQL
                     ERGHAYKDAVARGESPNAGIFNYPVLMAADILLYDTDVVPIGKDQKQHLELTRDVAMR
                     LNHLYGEGTLVVPEALIGEAPLVPGTDGEKMSKSKGNGIPLFESPKKLRKVIMGIKTS
                     SEGLDDPKRAEGSTVYELYSLVAPDRAPEMKQKLETGGYGWGHAKEDLYQAIEEEIGP
                     KREAYLSIRADEAKLDSILHAGADRARQIAHATIQRVRSAVGID"
     gene            complement(2579..3088)
                     /locus_tag="VFG22_18875"
     CDS             complement(2579..3088)
                     /locus_tag="VFG22_18875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418368.1"
                     /translation="MAKRLAWAALLIGLSGCLMQNLSPETRMRDAVVELNEGARWGRM
                     DVASGHVAPPFRPQFKLSHMRWGGDIQIADTEILGMNPHTDEEGDGAFSQIAVRWYDQ
                     STMVLADTVIRQTWKKHKQTFLLTNESVESGHPGLLVIPEPEQAPTPSAKVEEYENFE
                     DDSQWSTVY"
     gene            complement(3125..5857)
                     /locus_tag="VFG22_18880"
     CDS             complement(3125..5857)
                     /locus_tag="VFG22_18880"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6418369.1"
                     /translation="MSDVEHRPTVLMVGQGEPMQTALGEALRRHGVSVAACATAELEQ
                     AVRVYAPDLVVLIGDAAVRGGASVVRRMAANRATDVLPVAVVSNDAVLKHEGQDFRSG
                     AVAIVPRGAGADAIARRLAELADEVPQRPGLMSGEINEANLQDLVDLVSNDMKTGILS
                     IRSPRGVLEGMPMVVETERASAHNMELILDRLREAMEASQPLQYEFHETSGGRLSTLP
                     MATEPEDIDLSRLKDTRVVVLDSDELRAEKLAHVLHARGVQIAAADFSPAVLPRIREV
                     DPQVVVLDSGAVGGQGLDFVRAMRKDPQLRWSSMLVVRWQDFWPNSAYDAEPDLAQLA
                     ARILPMIEQDSEMSRRAEDEVDFDTRLELIGPGRLLRALGSVPGVRHLSIGGHKRSIE
                     IDIADNSIVGAYATLHEGTKQNVQGMPALLALWGLNSGRVSVREQDLPSVANIMMPVD
                     EALGLVSHQLGFADDRSGVEVDAATIPPAEPEVEEHATVPPGAEPPAKLEDARTMAPP
                     RFRHLQAAVGGPPAQAFSDEETPTNKVRRMLGRVETQKLTVPTGRHVTESAGDVSHTD
                     HAVTRASKSTQLGLAPVFEMLPQSALDEQKTIPVPRKPTPPPPPPESRKPAAPRLFEA
                     DEEFEPELARMLEGPVTLLAPAPAPAGMPAWIQLLLLLTLLAGVGVFGWQLWVRSDSA
                     LVAEDEQPASPAEAMADRQSEPITGTVNGLEPEAREDEDVEGVEPEPEPIAEEPTIAE
                     APEQAPEPVAVEAKAEQREQARPKREEKSPPAADSSTLAIPTQNLPRDPSKASDVLVH
                     RALPLIRKGQLRLAEATLDRAWELDPSNPQAMAGYAALYIAKKDGARAVKWAKKAVRK
                     RSRRAEYHVLYGDALQLEGESEAAQKSWRKALKVDPENKAARSRLNKANGGSAN"
     gene            5934..6569
                     /locus_tag="VFG22_18885"
     CDS             5934..6569
                     /locus_tag="VFG22_18885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HET6418370.1"
                     /translation="MNIAPIEFLRESFPKLFARGVEVLESRAAGGDERAQRILADVKG
                     VVGAASLQIDGAPPVFLSARSGALSAGEAPADGVPIKIIASLPGDAVQLLLGEVAKSG
                     ALENEEVAIGAAQTASKRFEDALAGRPMTCHVTLRGVPELGDVQVRLGFNVAEVPEAP
                     GFTTELQYSDLETVQRGELSVQELFLGGKLKMAGDYSLALQTAMQLLTNPL"
     gene            6688..>8082
                     /locus_tag="VFG22_18890"
     CDS             6688..>8082
                     /locus_tag="VFG22_18890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET6418371.1"
                     /translation="MFRAIAKSRYLVPGFLCFALAFVVGPVGVASGVLAGVQAASIAG
                     VVSAVLGVLGLLIIASGLPASLTIEEVDAQIRTIHGEIESVEGVIGENIKALSSGLGE
                     QMVAVEETARALKSMAISLQAISDRVEALSSSTEESSSSILQMTATNQEVAENMGNLG
                     SSVRETASSIEEMTYSIKEVARNVDALASTAEETSSSMNEMDVSIDQVQSNANETARL
                     SEEVAADAETGADSILKIIGEINRIKTSSLEAVSTISNLGDRIEAIGDILNVIDDVAE
                     QTNLLALNAAIIAAQAGEHGKGFAVVADEIKDLAERAGASTKEIAELIKTIQSETKNA
                     IEAVERGASTVDRGVTVSAEAERALKKILESSKQSTAMVRGIARATVEQAKGSRQVTD
                     AIGRIAETVQQIAAATAEQARGSELIMKSADNMRLITQQMERSSQEQAEGGRQMTGAI
                     ENISDMVLQLHQSHR"
BASE COUNT         1483 a         2517 c         2578 g         1504 t
ORIGIN      
        1 ctcgccggcc tgcgaagccg agtgttcctg ctccacaacg cccatgaaga agcggacgtc
       61 ttgttccaca cgcgctgggc gatgtcgtac ttgcgtgggc ccctgacacg taaagagatc
      121 aggcgcctga gcccgccgcc gagcgaccag gccacgagcc aaagtgacgc gcgcaaagcg
      181 ccacccagca ttcctgctgc gtcgagaagc gtacgtcccg tcgtcgcggc gggcatcgac
      241 gaagcgttct cgagtgacga acccggaacg tatcgtccgt atttggcggc ctcggtgtcc
      301 ctgcattacg tgcgcgcatc gcacggactg gatgcctggg aagagcgact gctgctcctg
      361 ccactcgatg aggagtcccc atgggaatcg ctcgcactgc tcgagcccga cgcgctcgat
      421 ctcgaacatc cggcgcctac cgaggccgag ttcgtcgaac cacgacccgg atcggtcggt
      481 aagacgatct tcagaagcta cggaaagcag atcaagagcc gtctatacca agatcgacct
      541 agagttcttt ggagctgcaa gcccctgaag ctgaaatcga cgcccgggga aaccaaggac
      601 cagtttgccg ctcgagccca gctcacgaaa cgcgaacacc gcgacgcggc gctcgagaag
      661 ctgagcgccg agctcgccaa gaagctgaag aaaatcgatg ccggaattcg gagagcagag
      721 gaccgggtcg aacgcgagaa gggccagtac gatcaggaga agctgcagac gggaatctcg
      781 atgggtgcga ccgtgttagg cgcgattcta ggtagccgta acatgggccg cgcgacgacc
      841 gctgcgcgtg gcgcgggtcg ggtcgccagg gagcgggagg acgtgcgtcg cgccgaggac
      901 gagttgcagg acctgctcgg ttcgcgtcgc gagctcgagc tcgaggccaa agaagaggtt
      961 cgcgcgcttc gggcggcaat ggacctcgaa tccgtggagc tcgaagaggt gctggtccgt
     1021 ccgaggaagt cggacatctc ggtgtccgac ctcaggctgg cctggcggct cgactgatct
     1081 tctcgaacgc ttcccgcaca tcctgcggag cctctacgag ctcgatgagc acgccctcgc
     1141 cgctgatcgg ggctgattca ttgcccttgg ggtggatgaa gcaaacgcga tggcctgctg
     1201 cgccgggccg tactccgccc ggagtgaatc gcacgcctcg atcaccgagc cacttcaccg
     1261 ctgcgtcgat gtcatcgacc cagagaccga tgtggttgag ggggggcgta tcgacccggg
     1321 gtttcttttc gggatcgatc ggttgcatca ggtcgacctc gacggtgaag ggcccggaac
     1381 ccatcgttac gatatcctcg tcgacgtttt ccttttcgct cttgaagttt ccgtgtgggg
     1441 tcagacccaa gatgtcgacc cagaggcggc gaagggcgct cttgtcggca gcgccgacgg
     1501 caacctgctg aattcccaag accttgaacg gacgagtcgt catgtttcct ctcttgacgc
     1561 gaccgaagat accccggttg ccggtcatgt ccacatggga cagtctcacg ccccgtgaag
     1621 acctctctca ccggcatcaa gcccacgcac cttccacacc tcggcaacta tctaggtgcg
     1681 attcggcccg cgctttcgct cgtcgaccgg taccgcacgc tctacttcat cgcggattac
     1741 cacgcggtca ccacggtccg cgatgcggac gcgctgcgcg gctacattcg cgatgtggcc
     1801 gcgacctggc tcgcctgtgg gctcgatccc gagcgaacct tgctctaccg ccagtccgat
     1861 gttcccgagg tcttcgagct cacgtgggtt ttggcctgtc tcttggccac cggacagctc
     1921 gagcgtgggc acgcttacaa ggacgccgtc gcacgcggcg agtcgccaaa cgcaggaatc
     1981 ttcaactatc cggtgctcat ggccgcggac attctcctct acgacaccga cgtggtgccg
     2041 atcggcaaag accagaaaca gcatctcgag ctcacgcgag acgtggcgat gcgcctcaat
     2101 cacctctacg gcgagggaac cttggtcgtg cccgaagcgc tcatcggcga ggccccgctg
     2161 gtcccgggga ccgacggcga gaagatgagc aagagcaagg gcaatggcat cccgctgttc
     2221 gaatcgccga agaagctccg caaagtgatc atgggcatca agaccagctc ggaaggactc
     2281 gacgatccaa agcgggccga agggtccacg gtgtacgagc tctacagtct ggtcgcgccg
     2341 gaccgggccc cagagatgaa gcagaagctc gagaccggtg gttacggctg gggccacgcc
     2401 aaggaagatc tctatcaagc catcgaagag gaaatcggtc caaagcgcga ggcatactta
     2461 tcgattcgcg ccgacgaggc gaagctcgat tcgattctgc acgcgggcgc tgaccgggcg
     2521 cgccagatcg ctcatgccac gatccaacgc gtgcggagcg cggtcggaat cgattgactc
     2581 agtagaccgt gctccactgc gagtcgtctt cgaagttttc gtattcttcc accttcgcgg
     2641 agggagtggg ggcttgctcc ggctcgggga tcacgagcag tcccggatgt ccgctttcca
     2701 ccgactcatt cgtgaggaga aaagtctgct tgtgcttctt ccaggtctgg cggatcacgg
     2761 tgtcggcgag gaccatcgtg ctctggtcgt accaacgcac cgcgatctgc gagaatgcgc
     2821 catcgccttc ctcgtcggta tgtggattca ttcccaagat ctcggtgtcc gcgatctgaa
     2881 tgtctccgcc ccagcgcatg tgcgagagct tgaactgtgg tcggaacggg ggtgccacat
     2941 gtccgctcgc tacatccatt cggccccacc gagcgccctc gttgagctcc acgaccgcgt
     3001 cgcgcatccg ggtctccggg ctcaggttct gcatcaggca tccagagagc ccgatgagaa
     3061 gagctgccca agcaagtcgt ttagccatgg gccgacgcta ggcgcgaccc tccggatagg
     3121 gcaactaatt ggcgctgccg ccgttggcct tgttgagccg cgaccgagcg gccttgtttt
     3181 cagggtcgac cttgagcgct ttgcgccatg acttctgcgc agcctcgctc tcgccctcga
     3241 gttgcagggc atcgccgtat aggacgtgat actcagctcg gcgcgaacgt ttgcggactg
     3301 ccttcttcgc ccatttgacg gcgcgcgcgc cgtccttctt cgcgatgtaa agggccgcat
     3361 agcccgccat ggcttgaggg ttgctgggat cgagctccca ggcccggtcg agcgtggctt
     3421 cggcgaggcg cagctggcct tttcgaatca ggggcagtgc gcggtgaacg aggacgtcgg
     3481 aagctttcga gggatcgcgc gggagattct gagtcggaat cgccagagtg cttgaatccg
     3541 ccgcaggcgg gctcttttcc tcgcgtttgg gcctcgcttg ttcacgctgc tccgctttgg
     3601 cctcgaccgc gacgggctcc ggcgcttgct ccggagcctc ggcaatcgtg ggctcttcgg
     3661 cgatgggttc cggctccggc tccaccccct cgacatcttc gtcttccctg gcctcgggct
     3721 ctagtccgtt gacggtcccc gtaatcggtt cgctttggcg atctgccatc gcttcggccg
     3781 gtgatgcggg ttgctcgtcc tctgcgacta gggccgagtc actgcgcacc cagagctgcc
     3841 agccaaatac gccgacgcca gcgaggagcg tcagcaagag caggagttga atccaagcgg
     3901 gcatgccagc cggggccggg gctggcgcca gcagggtgac agggccttcc agcatccgcg
     3961 caagctccgg ttcgaactcc tcgtccgctt cgaacaaacg gggcgctgcg ggctttcggc
     4021 tttcgggcgg tggcggcggc ggcgtgggct ttcgaggtac gggaatcgtc ttctgctcat
     4081 cgagcgcgct ctgtggaagc atctcaaaca cgggcgccaa cccgagctgg gtgctcttcg
     4141 acgcccgagt caccgcatgg tccgtgtggg aaacgtcgcc ggcgctttct gttacgtgac
     4201 ggccagtcgg gacggtcagc ttttgtgtct ccacccggcc gagcatccgg cgcaccttgt
     4261 tggtaggggt ctcctcgtcc gagaacgctt gcgcgggtgg gcctccgact gcggcctgga
     4321 gatgtcgaaa acgtggtggc gccatcgtcc gcgcgtcttc caacttcgcc ggaggttcgg
     4381 ctccaggcgg gactgttgcg tgctcttcga cctccggctc tgccggcgga atcgttgctg
     4441 cgtcgacttc gacaccgctt cgatcgtcgg cgaagccgag ttgatgcgag acgagcccga
     4501 gggcttcgtc cacgggcatc atgatgttcg cgaccgaagg aagatcttgt tcgcgcaccg
     4561 acacgcggcc cgagttgagg ccccaaagcg cgagaagcgc tggcatgcct tgcacgttct
     4621 gcttggtgcc ctcgtgcagc gtcgcgtagg cccccacgat cgaattgtcg gcgatgtcga
     4681 tctcgatact acgcttgtgc ccgccgatcg acaggtgcct cactcccggc acggagccca
     4741 gtgcgcgcag cagacgtcct ggcccgatga gctcgagtcg ggtgtcgaag tcgacctcgt
     4801 cttcggccct acggctcatc tcgctgtctt gttcgatcat cggcaggatc cgagcagcga
     4861 gctgcgcgag gtccggctcg gcgtcgtacg cggagttcgg ccagaaatcc tgccagcgca
     4921 cgaccagcat cgaagaccaa cgcaattgcg gatcttttcg catggctcga acgaaatcga
     4981 gaccctgtcc gcccacggcg cccgagtcca agacgacgac ttgcggatcg acttctcgaa
     5041 tcctcgggag gacggcgggc gagaaatcgg ccgccgcgat ctgaacgcct cttgcgtgca
     5101 gaacgtgtgc gagcttctcg gctcggagct cgtccgaatc gagaacgacg acccgcgtgt
     5161 ccttgaggcg cgacaggtcg atatcttcgg gctcggtcgc catggggagg gtcgagagcc
     5221 ggccaccgct cgtttcgtga aactcatact gcagcggctg ggaggcctcc atcgcctcgc
     5281 gcaagcgatc gagaatgagc tccatgttgt gtgccgaagc ccgttccgtc tccacgacca
     5341 tcggcatgcc ttcgagcacg cccctcggcg agcgaatgct gaggatacct gtcttcatgt
     5401 cgttcgaaac gaggtcgacc aggtcttgaa gattggcttc gttgatctcg cccgacatca
     5461 ggcccgggcg ctgagggacc tcgtcggcaa gctcggcgag ccgtcgcgca atggcatccg
     5521 cgcccgcgcc ccgagggacg atcgccacgg cacccgaccg aaaatcctgc ccctcgtgct
     5581 tgaggaccgc gtcgttcgag acgacggcga cgggcagaac atcggttgca cgattcgctg
     5641 ccatacgacg tacgacgctc gcgccgccac gaactgcagc atcaccgatc aaaacgacca
     5701 agtccggggc gtacacacgg accgcttgtt cgagctccgc cgttgcacaa gcagcgacgg
     5761 atactccatg gcgccgaagc gcttccccca acgctgtttg catcggctcg ccctggccca
     5821 ccatcaagac agtaggccgg tgctccacat ccgacattgc agagatggtg gccgattatt
     5881 tcggatttcg ccagcttggt gctcaaggcg atgttggcta tgctccgcgc cgcatgaaca
     5941 ttgcacctat tgagttcctt cgggagagct tccccaagct ctttgcccgg ggggtggaag
     6001 tcctcgagag ccgcgctgcc ggcggcgatg agcgggctca gcgaatcctg gccgacgtga
     6061 agggcgtcgt tggagcggca agccttcaga tcgatggcgc gccccccgtg ttcctttccg
     6121 cgcgtagtgg agcactgagt gctggggagg ccccggctga cggagttccg atcaaaatca
     6181 tcgcctcgct tccaggcgac gccgtacagc ttctgctcgg agaggtcgcg aagtccgggg
     6241 ccctcgagaa cgaggaggtg gccatcggcg cagcacaaac ggcatcgaaa cggttcgagg
     6301 acgcgctggc tgggaggccg atgacctgtc acgtgacgct tcgcggcgtg cccgagctcg
     6361 gtgatgtcca ggttcggctc ggcttcaacg tcgcggaggt gccagaagcg cccggcttca
     6421 ccaccgagct ccaatattcc gacctcgaga ccgttcagcg aggagagctc agcgttcagg
     6481 aactgttttt gggcggcaag ctcaagatgg cgggggacta ttcgcttgcc ctgcagacgg
     6541 cgatgcaact cctcaccaac cccctctgaa cggaagatcc cctctgctgc gtgccaatcc
     6601 tcctgaaatg gcgggggaac ttgcccgcgg ctcctctggt tcgataccct gacgcttgtc
     6661 tagttcgtct ggttcgccgg gggggtcatg tttcgggcaa tcgccaagag tcgctatctg
     6721 gttccgggct tcctgtgctt cgcgctggcg ttcgtcgtgg gccctgtcgg ggtagcgtcc
     6781 ggcgttctgg cgggggttca agcggcttcc atcgcaggcg tcgtctccgc ggtgctcggc
     6841 gttctcggct tgttgatcat cgcttctggc ttgccggcgt cgctcacaat cgaggaggtc
     6901 gatgcgcaga ttcgcacgat ccacggtgag atcgaatcgg tcgaaggcgt gatcggagag
     6961 aacatcaaag cgctttcgag cggcttgggc gagcaaatgg tcgccgtgga agagaccgcc
     7021 cgcgccctca agtccatggc gatctcgctg caagcgatca gtgaccgggt cgaggcgctt
     7081 tcatcgagca ccgaagagag ctcctcgagc atcctccaga tgacggcgac gaaccaggaa
     7141 gtggccgaga acatgggaaa cctcgggtcc agcgtccgcg agacggcgtc ttccatcgag
     7201 gagatgacct acagcatcaa ggaggtcgcg cggaacgtcg atgccctcgc atcgaccgcg
     7261 gaagaaacct cctcgtcgat gaacgagatg gacgtttcaa tcgatcaggt tcaatcgaat
     7321 gcgaacgaga ccgcccggct ttcggaggag gtcgcggccg acgcggagac cggagccgat
     7381 tcgattctga agatcatcgg ggagatcaat cgcatcaaga cttcgtcact ggaggcggtg
     7441 agcacgattt caaacctcgg tgaccgaatc gaggcgattg gcgacatctt gaacgtcatc
     7501 gacgacgttg ccgagcaaac gaacctactc gctttgaacg cggcgatcat agcggctcag
     7561 gcgggtgaac atggcaaggg tttcgccgtg gtcgccgacg agatcaagga cctcgcggaa
     7621 cgagccggcg cgtctaccaa ggagatcgcc gagctgatca agaccatcca gtcggagacc
     7681 aagaatgcca tcgaagcggt cgagcgtgga gcgagtaccg tcgaccgagg tgtgacggtg
     7741 agcgccgagg cagagcgtgc gctcaagaag atcctcgaat cgtcgaagca gtcgacggcg
     7801 atggtacgtg gaatcgcgcg cgctacggtc gagcaggcca agggctctcg ccaagtgacg
     7861 gatgcgattg gtcgtattgc ggagacggtt cagcagatcg cggcggcgac cgccgagcag
     7921 gcacgcggct cggagctcat catgaagagc gcggacaaca tgcgattgat tacgcagcag
     7981 atggagcgca gcagtcagga gcaggccgag ggtggccgcc agatgaccgg cgcgatcgaa
     8041 aacatttccg acatggtgct tcaactccat cagtctcacc gc
//
LOCUS       DASRZF010000430        10774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2058558, whole genome shotgun sequence.
ACCESSION   DASRZF010000430 DASRZF010000000
VERSION     DASRZF010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 10774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10774
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2058558"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VFG22_18895"
     CDS             complement(<1..211)
                     /locus_tag="VFG22_18895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418372.1"
                     /translation="MKRLLSCLCFVVSASLFGAAIAQSTPGFIHVDEIRPGMKGYGLS
                     VFRGTAPERFDVEVIDVLHNFRPDQD"
     gene            complement(232..672)
                     /locus_tag="VFG22_18900"
     CDS             complement(232..672)
                     /locus_tag="VFG22_18900"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6418373.1"
                     /translation="MASKASSQKLESAARTIAESGPRHRSVLLVESDPDLQWSLARML
                     TVDGNRVVGTSSGEGALTVIEQWDADLALVAAQLPGDDGVEVARQLRAHNPDLVVILI
                     GEAAPEPVSKERSTDVSAYLPRPFRFEALRALIESLQLTPAPAE"
     gene            complement(839..3058)
                     /gene="fadJ"
                     /locus_tag="VFG22_18905"
     CDS             complement(839..3058)
                     /gene="fadJ"
                     /locus_tag="VFG22_18905"
                     /EC_number="1.1.1.35"
                     /EC_number="4.2.1.17"
                     /EC_number="5.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HET6418374.1"
                     /translation="MAHEGLSIEKRGDGVAIVRMDIPGEPMNTLKSDFVDTFMEVFGS
                     IESDPEIKAVVFTSGKKDSFIAGADISMLESVSTAEEGERISREGHKAMNRIESCPKP
                     VVAAIHGVALGGGLEVALACHARVASDSKKTKLGLPEAQLGLLPGAGGTQRLPRLIGV
                     QPALDLMLTGKQVNAKKAKKLGLVDDVVPPSILLETAAQLALSRIGYRPKRESFIDQL
                     RDKDAMTELALAKNPVGRKILFDQARKQLHKQTRGNYPAQDLIIDVVKAGLEDGFEAG
                     LQAEARGFGQLLMTTEAANLMSLFFATTALKKDSGVDDPNAKPREIKKVGILGAGLMG
                     AGIAYVTTSLAKLPVRLKDRDSDGVMAGLRYIRGIIDGGVKRKRLSEQQANHLMLQTS
                     GTTSYAGFHEIDIVIEAVFEDLSLKERMVRDVEEAGRDDVIFASNTSSLPITKIAAAS
                     KHPETVIGMHYFSPVHKMPLLEIIVTDKTADWVTATAVDLGKRQGKHVIVVRDGVGFY
                     TSRVLAPMMNEAAHLVAEGVPIEKIDSAMLDWGFPVGPIKLTDEVGIDVGAKVGKIML
                     EAFGERMSAPEGMKKLIDDERFGRKNGRGFYLYGKKKKGVDESVYEVLGVTPSNKSIR
                     NEDIAWRCALQFVNEACRCFGEGILRSARDGDIGAVFGLGFPPFRGGPFRFVDQVGAK
                     EVLGRLRELEKQLGPRFSPAPVLEEIARSGQTFHGPNPIQPGQSRGNKSQASASATA"
     gene            complement(3058..4350)
                     /gene="fadI"
                     /locus_tag="VFG22_18910"
     CDS             complement(3058..4350)
                     /gene="fadI"
                     /locus_tag="VFG22_18910"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014948809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HET6418375.1"
                     /translation="MESNGKRVAIVAGLRTPFAKQWSAYREVSALDLANIVVAELLQR
                     IDLDPKEIQQVVYGQVVPSVEAPNIAREVVLATGMPKSIEAYSVSRACATSYQSAVNV
                     AEAIMAGTIDTGLAGGADSASNVPITVSKRLAEALIAATKARSLGERMQAFAGLRPRD
                     LAPVPPAIKEFSTGLSMGESAEKMAKENHISREAQDEFALRSHSLAAKAWAEGKFDDE
                     VMEVFVPNRFKETIREDNLVRKDGEIEKYAKLKPAFDREHGTVTAGNSSPLTDGASAL
                     VLMREDKAKSLGLDVLGFIRSYAFAALDPAGQLLMGPSYASPLALDRAGVKVSDLDLI
                     DMHEAFAAQILSNTQAFESKEWAEKNLGRSEKIGEIDWDKFNVTGGSISIGHPFAATG
                     ARQITQTLRELKRRGGNLALCTACAAGGLGAAMVLEAE"
     gene            4472..5545
                     /gene="moaA"
                     /locus_tag="VFG22_18915"
     CDS             4472..5545
                     /gene="moaA"
                     /locus_tag="VFG22_18915"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HET6418376.1"
                     /translation="MRHLPLLQSRSAVYEDWPARPDDFDAIHFLRDARGRRYTYLRLS
                     VTDRCDLACVYCMPPSGEDDHAIRRDMLTFEEAARLVSVFAAAGIRRVRFTGGEPLVR
                     KDIVRLVELVHRRTEVEQLVMTTNATRLSELARPLREAGLHGVNVSIDTLDAQRFFEI
                     TRGGDLGRVLAGIHAALDVGLEVKLNTVPLRGQNDDELSNIVDFAWSLGATPRFIELM
                     PLGEGAKLPKSMRMNAADVLEQLADHVGQETAIGVEGQGPARYLAARDGSGHRVGFIT
                     PISDEFCDTCNRVRVTAKGDIRACLASRRAISLRNIMRAGGSDLDLAWAMVWSLSGKD
                     RGHHFLEPEEHEHERVGMSLIGG"
     gene            5561..5962
                     /locus_tag="VFG22_18920"
     CDS             5561..5962
                     /locus_tag="VFG22_18920"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET6418377.1"
                     /translation="MLIRMMVACLLMLGCSSEPANLRVWTPEDHAHAPDTEIDPNRVP
                     QLERPDLTVAELLWQRNCARCHGTDGQGGRDAKISFASAAWQDGIDDATIARTITGGN
                     PPMMPAFGELLTPEQIGVLVKHVRTLAATTE"
     gene            complement(6037..6963)
                     /gene="prs"
                     /locus_tag="VFG22_18925"
     CDS             complement(6037..6963)
                     /gene="prs"
                     /locus_tag="VFG22_18925"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HET6418378.1"
                     /translation="MKQATLLFATERYQELGEDIAGAGDFDLGVVERKVFPDTERYRR
                     IDTDCARRDVVLVGGTVDDADTLEIYDLACGLVHLGVHTLTLVLPWFGYQTMERASKH
                     GEIVAAKNRARLLSSIPAAGSGNRAVLLDLHSEGIPQYFSGDIRPVHLQARSVVLQAI
                     RDFGGEDFVLGATDAGRAKWVESLANDLLVDAAFVFKRRLSDTETEVTALAADVEGRP
                     VVIYDDMIRTGGSLISAALSYKDAGASEVYAVCTHGVFAEESLSRIRDSGALEGLACT
                     NTRPHVQALADGDFLRVYSVADLIAQFLIDSV"
     gene            complement(7001..7792)
                     /locus_tag="VFG22_18930"
     CDS             complement(7001..7792)
                     /locus_tag="VFG22_18930"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HET6418379.1"
                     /translation="MPRPYFGPTTPLAFAHRGGSKRWPENTLLAFRNAFELGYRQIET
                     DIHETSDGRFVCFHDPTLERTTNGRGNLRDLSLAELQRLDAGHNFLEAGGYPFRGAGA
                     RIPTLEEILDLPSELHYTLEIKPNDPALARRLWEFIDGHGIHDRVLVASGHDEVTDAF
                     RTASRGRVATSAGQQRARAFWARVLTGSWKHAMFPFDALQVPPRYHGIKLITPRFVEA
                     AHHHGVQIHVWTIDDPAEMRELLDTGADGIMTDVPDLLLEVMKEY"
     gene            7888..8715
                     /locus_tag="VFG22_18935"
     CDS             7888..8715
                     /locus_tag="VFG22_18935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6418380.1"
                     /translation="MAKDLSGKVAVITGGAGGLGKALGEEMAARGCYLVLADINRDLL
                     EATAAALRASGAQVDARTVDVRDAAAVQALVDDAFRELGRIDYLFNNAGVNLCAELRD
                     TTLEDWDLLIDVNFRGVIHGVHAAYPIMREQGFGHIVNVASAAGLIPAAAEGAYAATK
                     HAVVGLSSSLRIEAESFGVKVSVVCPGLIDTPILDSTKYVNFDTDAITSVSPEKPMEP
                     RRAARLILRDVDRNRFFIVLSATAQVLWRVHRYAPAASIWLGKLGVRRFRGIRTDAR"
     gene            complement(8718..9662)
                     /locus_tag="VFG22_18940"
     CDS             complement(8718..9662)
                     /locus_tag="VFG22_18940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HET6418381.1"
                     /translation="MFDPSKTLVIANPMARHGFVGANWPELSARIREGLGPVELQLTA
                     SRGDGVRIGKDAIARGVRTIVSFGGDGTHSEVVDAIMQSGANKELTLGILHAGTGGDF
                     RKMIPGAEEIESGCAAIREKPAVPIDIGWVQYVHDEGHTESRFFLNITSMGMGGLVDR
                     FVAASPSKSSGKAKYFKAVLRAQMKYKPARIRMRIDGKDEGEHEISNLCVCNGRWAGG
                     GMMFAPEAHVADGMFDVILLRAASTLRGLPVMARLYKGTHVRSPIVSTYRGRHIEVEM
                     LENTAWMDIDGEAPGVGPAEFRIHAGALRVIGIASEFL"
     gene            9712..10635
                     /locus_tag="VFG22_18945"
     CDS             9712..10635
                     /locus_tag="VFG22_18945"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HET6418382.1"
                     /translation="MLSRIWVFVFALACTGCGSDPASSVCEPLTAGECAEPDAGVPDG
                     GSPLESSVVVDVDWLEARLGDPDVQLIDTRSFGYEASRIPGAIELRPSALAKAIDGVS
                     SQLVSAIEAQPVLRAAALRNDVTALVYGGSPEYDAARVVWALRYYGHLDVRYLNGGFA
                     AWVEAGGALDTDPPNIEPSEYSIVAVNEDLLVRGDWVLSQLGEAPYEAPAIQLVDARS
                     SGEYEAGHIPSARSVNWSQNLDRGLLRSRTELEALYSGLAPDRTTVTYCASGLRGSFA
                     WLTLSALGYSDARLYDGSWNEWGAGGFPVEQ"
BASE COUNT         1854 a         3394 c         3430 g         2096 t
ORIGIN      
        1 agtcctgatc agggcggaag ttgtggagga catcgatgac ttcgacgtcg aagcgctcgg
       61 gagccgtgcc ccgaaatacc gagagaccat agcccttcat ccccggacga atctcgtcga
      121 cgtgaatgaa gcccggtgtc gattgggcaa tggcggcgcc gaagagcgac gcgctcacta
      181 cgaaacacaa gcaggagagc agccgcttca cgccgaagaa gcctagcagg cctactccgc
      241 gggggccggt gtaagttgga gcgactcgat cagcgcgcgc agcgcctcga atcgaaacgg
      301 cctgggcagg tatgcgctca cgtcggtgga ccgctctttg gagaccggct ctggcgccgc
      361 ttcaccgatg agaatgacca cgaggtcggg attgtgcgcc cggagctgtc gcgcaacctc
      421 cacaccgtcg tcgcccggca actgcgccgc gaccagggcg aggtcggcgt cccattgctc
      481 gatcaccgtg agtgcacctt cccccgagct cgtgcccacg acacgattgc cgtcgaccgt
      541 gagcattctc gcgaggctcc actgaaggtc tggatcgctt tcgacgagca agacgctgcg
      601 gtgacgggga ccgctttccg cgatcgtccg tgctgcgctt tcgagcttct gagagcttgc
      661 cttgcttgcc atcttcggtg cccagtcgct ctttggctga gacaacgaac caacgcccca
      721 cgcgcgtccg ttgttcccac ctccccgacc gtcaaacatc ataaagaggt cagggctcga
      781 gtgcaatcga cccctccgac tatcagcgaa tcagccgata gtccggtgaa atccactgct
      841 aagctgtcgc gctcgcagag gcctgggact tgtttccacg ggactggccc ggctgaatag
      901 gattgggtcc gtggaaggtc tgtccgctcc tggcgatctc ctcgagcacg ggcgcgggcg
      961 agaaccgagg tccgagttgc ttctcgagct cgcgtaggcg tccgagaacc tccttggcgc
     1021 cgacctgatc gacgaagcgg aagggcccac ctcggaaggg aggaaagccg agcccgaaga
     1081 ccgcgccgat gtcgccgtcg cgtgcgctgc gcaagatgcc ctcaccgaag cagcggcagg
     1141 cttcgttcac gaactgaaga gcgcagcgcc aggcgatgtc ctcgttgcgg atgctcttgt
     1201 tgctgggggt gacccccagg acctcgtaga cggattcgtc gacgcccttc ttcttcttgc
     1261 cgtagagata gaagcctcgc ccgttcttgc ggccgaagcg ctcgtcgtcg atgagcttct
     1321 tcattccttc cggggcgctc atgcgctcgc caaaggcctc gagcatgatc ttgccgacct
     1381 tggctcccac gtcgattccg acttcgtccg tgagcttgat cgggccgacc ggaaagcccc
     1441 agtcgagcat ggccgagtcg atcttttcga tcggcacccc ttcggctacc aggtgcgccg
     1501 cttcgttcat catgggcgcc agtacgcggc tcgtgtagaa cccgacgcca tccctcacga
     1561 cgatgacgtg cttgccctgt cgcttgccga ggtcgaccgc ggtcgcggtg acccagtcgg
     1621 ccgtcttatc ggtgacgatg atctcgagca gcggcatttt gtggaccggt gagaagtagt
     1681 gcatcccgat tacggtctcg gggtgcttgc tggccgccgc gatcttggtg atcggaagcg
     1741 aggaggtgtt cgacgcgaaa attacgtcgt cacggcccgc ttcttccacg tcgcgaacca
     1801 tccgctcctt cagcgaaagg tcttcgaaaa ccgcctcgat gacgatgtcg atctcgtgga
     1861 agccggcgta gctcgtggtt ccgctcgttt gcagcatcaa gtgattcgcc tgctgctcgc
     1921 tcagtcgctt gcgcttcacg cctccgtcga tgatcccgcg gatgtagcga aggccggcca
     1981 tcacgccgtc gctgtctcgg tccttgagtc ggaccggcag cttcgcgagc gatgtcgtga
     2041 cgtaggcgat gcctgcgccc atgaggcccg cacccaggat tccgaccttc ttgatttcgc
     2101 gcggcttcgc gttcgggtcg tcgacaccgg agtccttctt cagcgcggtc gtggcgaaga
     2161 agaggctcat caggtttgcc gcttcggtcg tcatcaagag ctggccgaag ccacgagcct
     2221 ctgcctggag acctgcttcg aagccgtctt cgagaccggc tttcacgaca tcgatgatca
     2281 gatcctgggc cgggtagttt ccgcgcgtct gtttgtgaag ctgcttgcgc gcctggtcga
     2341 agaggatctt tcgtccaacc gggttctttg caagcgcgag ctccgtcatc gcgtctttgt
     2401 cgcgaagctg gtcgatgaag ctttcccgct tgggtcgata cccgatgcgc gagagcgcga
     2461 gctgcgcggc ggtttcgagc aggatcgacg gcggaactac gtcgtcgacg aggccgagct
     2521 tcttggcctt cttggcgttc acctgcttgc cggtcagcat caggtcgagc gctggctgaa
     2581 cgccgatgag cctcgggagc cgctgcgtac caccggcacc cgggagcaag ccgagctggg
     2641 cttcgggaag accgagcttg gtcttcttgc tgtcgctcgc gacgcgcgca tggcacgcca
     2701 gggcgacctc gagtccgccg ccgagcgcga cgccatggat cgccgcaacg acgggctttg
     2761 ggcagctctc gattcgattc atggccttgt ggccttcacg cgagatacgc tcgccttcct
     2821 cggccgtgct gacgctctcc agcatgctga tgtcggcgcc ggcgatgaag ctgtccttct
     2881 taccggaggt gaagaccacc gccttgatct cggggtcgct ttcgatcgag ccgaagacct
     2941 ccatgaaggt gtccacgaaa tcgctcttca gcgtgttcat cggctcgcca ggaatgtcca
     3001 tgcgaacgat ggctacgccg tcgccgcgct tctcgatgct cagtccttcg tgcgccatta
     3061 ctctgcctcc aaaaccatgg ctgcgcccag gccacctgcg gcgcaggcgg tgcagagggc
     3121 caggttgcca ccgcgcctct tgagctcgcg gagcgtttgt gtgatctgac gtgcgccggt
     3181 cgcggcgaat ggatgtccga ttgaaatcga gccgccggtc acgttgaact tgtcccagtc
     3241 gatttcgccg atcttttcgg agcggcccag gttcttctcc gcccattcct tggactcgaa
     3301 tgcctgcgtg tttgacagga tctgcgcggc gaatgcctcg tgcatgtcga tgagatcgag
     3361 atcggaaacc ttcacacctg cgcggtcgag ggcgagcggt gaagcgtagg acgggcccat
     3421 caggagctgc ccggctgggt ccagcgccgc gaaggcgtag ctccgtatga agccgagcac
     3481 gtccagacca agcgacttgg ccttgtcctc tcgcatgaga accaatgcgc ttgcgccgtc
     3541 ggtgagcggc gagctgttgc cggcggtgac ggtgccgtgt tcccggtcga aagcgggctt
     3601 gagcttcgcg tacttctcga tttcgccgtc tttgcgaacg aggttgtcct cgcggatcgt
     3661 ctccttgaag cgattcggca cgaatacctc catcacctcg tcgtcgaact tgccttcggc
     3721 ccaggccttg gctgcgagcg aatggcttcg gagagcaaac tcatcctgag cttcccggct
     3781 gatgtggttt tccttcgcca tcttctctgc gctctcgccc atcgacaagc cggtcgaaaa
     3841 ctccttgatg gcgggtggca ccggggcgag atcgcgcggc cgcagtcctg cgaaggcctg
     3901 cattcgctcc ccgaggcttc gggccttggt cgcggcgatg agagcctcgg cgagccgctt
     3961 cgaaacggtg atcggaacat tgctggcgct gtcggcgcca cctgcgagcc cagtgtcgat
     4021 cgtgccggcc atgatcgctt cggccacgtt gaccgcggac tggtagctcg tggcgcaggc
     4081 gcgggaaaca ctgtaagcct cgatgctttt cggcattccg gtcgccaaga cgacctcgcg
     4141 cgcgatgttc ggcgcctcca ccgaaggcac cacctgaccg tagacgacct gctgaatctc
     4201 cttggggtcg aggtcgatcc gctgcagcag ctcagccacc acgatgttgg cgaggtcgag
     4261 agcgctgacc tctcgatatg ccgaccattg cttcgcgaaa ggggttcgca atccggcgac
     4321 gatcgccacg cgctttccat tcgattccat gcgcagtgct cctcaaaatg aacagtgatt
     4381 cagtgaaggg gagagcctga aacccggccc gagtgaagtc aaggcagcgt gcgcccgttg
     4441 accggggcgc ggcggcccct agactcgtgc ggtgcgtcac ctccccctcc tgcaaagccg
     4501 gtccgcggtg tacgaagact ggcccgcacg gcccgacgat ttcgacgcga ttcacttctt
     4561 gcgcgatgcg cgcgggcgcc gatacacgta cctgcgactc agcgtgaccg atcgctgcga
     4621 tcttgcatgt gtgtactgca tgccgccgag cggcgaagac gatcacgcga tccgccgcga
     4681 catgctgacc ttcgaggaag cggcgcggct ggtgtcggtc ttcgcggctg cggggatacg
     4741 ccgggttcga ttcactggcg gagagccctt ggtgcgcaaa gacatcgtac gcctggtcga
     4801 gctcgtgcac cggcgcaccg aggtcgagca actggtgatg acgacgaatg cgacccgcct
     4861 gtccgagctt gcgcggccac ttcgcgaagc aggtctccac ggggtgaacg tgtcgatcga
     4921 tacgctcgat gcgcagcgct ttttcgagat cacccgtggc ggcgatctgg ggcgcgttct
     4981 ggccggcatt cacgcggcgc tcgatgtggg gctcgaggtg aagctcaaca cggttccgct
     5041 tcgcggccaa aacgacgacg aactgtcgaa tatcgtcgat ttcgcttggt cgctcggcgc
     5101 gacgccccgt ttcatcgagc tgatgccgct cggcgagggc gccaagcttc cgaagagcat
     5161 gcggatgaac gccgcggacg tgttggagca acttgctgat catgtcggtc aggaaacggc
     5221 gatcggagtc gaaggccagg ggcccgcgcg ctacttggcg gcccgagatg gctctgggca
     5281 tcgcgttggt ttcatcacgc cgatcagcga cgaattttgc gacacctgca atcgcgttcg
     5341 ggtgacggcg aaaggcgaca ttcgtgcgtg tctggcctcg cgtcgcgcga tcagccttcg
     5401 aaacatcatg cgggccggcg gctccgacct ggatctggcc tgggccatgg tctggtctct
     5461 ttcgggcaaa gaccgtgggc accattttct cgaacccgaa gagcacgagc acgagcgcgt
     5521 cgggatgagt ctcatcggcg gataggctac agggggcgtt gtgttgatca ggatgatggt
     5581 ggcctgcctc ttgatgctgg gctgttcgag cgaacccgcg aaccttcgcg tttggacccc
     5641 ggaggaccat gcgcacgcgc ccgatacgga aatcgacccc aaccgcgtgc cgcagttgga
     5701 acgccccgac ctgaccgtcg ccgagctcct ctggcaacga aactgcgcga ggtgtcacgg
     5761 aacggacggt caaggcggcc gcgacgccaa gatcagcttc gcgagcgcag cctggcaaga
     5821 cggcatcgac gacgcgacga tcgcgaggac gatcactggg ggcaatcccc cgatgatgcc
     5881 cgccttcggc gagctgctga cacccgagca gatcggcgtg ctggtgaagc atgtgagaac
     5941 gctcgctgcg accaccgagt aagctcagcc tcagtgccag tgccagtgtc agtgccagtg
     6001 tcggtgtcgg tgtcagcgcc agcgccagcg ccagcgttag acgctgtcga ttaggaactg
     6061 cgcgatcagg tcggctacgg agtagaccct caggaagtcg ccgtcggcta gcgcttggac
     6121 gtgtgggcgc gtgttggtgc aggcgaggcc ttcgagagcg ccgctgtcgc ggatcctgct
     6181 gagcgattcc tcggcgaaga cgccgtgggt gcagaccgca tagacttcgc tggcgcccgc
     6241 gtctttatag gagagcgccg ccgaaatcag cgagccaccg gtgcgaatca tgtcgtcata
     6301 gatgacgacg ggacggccct ccacgtcggc agcaagcgcg gtgacctcgg tctccgtgtc
     6361 cgagaggcgg cgtttgaaca cgaacgcggc atccaccaag agatcgttgg caagactctc
     6421 gacccatttc gcgcggccgg cgtcggttgc gccgagaacg aagtcttcgc caccgaagtc
     6481 ccgaatcgct tgcaagacca cggatcgtgc ctgcaagtgg accgggcgaa tgtcgccgga
     6541 gaagtattgt ggaatgcctt ccgaatggag gtcgaggagc acggcgcggt tgcccgagcc
     6601 cgcggccgga atcgatgaaa gtagacgagc tcgattcttg gcggcgacga tctcgccgtg
     6661 cttcgaagcg cgctccatcg tctggtagcc gaaccagggc agcacgaggg tgagcgtgtg
     6721 caccccgaga tgaaccagac cgcatgccag gtcgtagatc tcgagcgtgt ccgcgtcgtc
     6781 gaccgttccg ccaaccaaga cgacgtcgcg ccgggcgcaa tcggtgtcga tgcggcggta
     6841 tcgctccgtg tcggggaaaa ccttgcgctc cacgacgccc aggtcgaagt cgcccgcgcc
     6901 ggcgatgtct tcgccgagct cttggtagcg ctcggtcgca aataggagcg tcgcttgctt
     6961 catcttggtg ctctcatttc agcatgggcg ctttcacaga ctaatactct ttcatgactt
     7021 cgagcagcag atccggaaca tcggtcatga ttccgtcagc ccccgtgtcg aggagctctc
     7081 gcatctcggc ggggtcgtcg atcgtccaga cgtgaatctg aacgccgtgg tggtgagccg
     7141 cctcgacgaa ccgaggcgtg atcagcttga tgccatggta tctgggaggc acctgaagag
     7201 cgtcgaaggg aaacatcgcg tgcttccagg atcccgtcaa cacgcgcgcc cagaaggccc
     7261 ttgcgcgttg ctgacctgcc gaggtggcga cccgaccgcg actcgctgtg cgaaacgcgt
     7321 cggtcacctc gtcgtgcccc gaggccacga gcacccggtc atgaatgcca tgcccatcga
     7381 tgaactccca aagccgccgc gccagtgcgg gatcgttggg tttgatctcg agtgtgtagt
     7441 gaagctcgga gggaaggtcg agaatctctt cgagggtcgg tattcgcgcg cccgctcccc
     7501 gaaacgggta gcccccggct tccaagaagt tgtgcccggc gtcgaggcgc tgcagctcgg
     7561 ccaagctcag atcgcggagg ttcccccggc cattggtcgt gcgctcgagc gtcggatcgt
     7621 ggaagcagac gaaccggcca tctgaagttt cgtgaatgtc ggtttcgatt tgccgatagc
     7681 cgagctcgaa tgcgtttcga aatgctagca gcgtgttttc gggccagcgt ttcgagcccc
     7741 cacggtgcgc gaaagccaag ggcgtcgtcg gtccgaagta ggggcggggc atcgcgttgc
     7801 gagcatagcg ctctttttct cgtgaggcgc acaacacttg tccctcatcg attttctgcg
     7861 catcgtgcgc cccgaaggag cccgaacatg gcgaaggatc tgtctggaaa ggttgccgtc
     7921 atcaccgggg gcgctggcgg cctcggaaag gctctcggag aagaaatggc agcgcgtggc
     7981 tgctatctgg tgctggccga catcaatcgc gacttgctcg aggcgacggc tgctgcgctt
     8041 cgagcatccg gcgctcaggt cgacgcgagg accgtcgacg tgcgtgacgc cgccgccgtt
     8101 caggcgctcg tcgacgacgc gtttcgagag ctcggccgca tcgactacct cttcaacaac
     8161 gcaggcgtga atctctgcgc cgagcttcga gacacgaccc tcgaggactg ggatctgctc
     8221 atcgatgtga actttcgcgg cgtcattcac ggcgtccacg cagcgtatcc gatcatgcgg
     8281 gagcagggct tcggccacat cgtcaacgtc gcttcggccg ccggactcat cccggccgca
     8341 gcagagggcg cttacgcggc gaccaagcat gcggtggtgg ggctttcctc gtcgctccgc
     8401 atcgaggcgg aatcattcgg cgtcaaggtg agcgtcgtct gccccggtct gatcgacacc
     8461 ccgattctcg attcgacgaa gtacgtgaac ttcgataccg acgccatcac gtcagtctcg
     8521 ccggagaaac cgatggaacc gcgcagggcg gcgcggctca tcctgcgcga cgtcgatcga
     8581 aatcgcttct tcatcgtact cagcgccacg gcgcaggtac tctggcgagt tcatcggtac
     8641 gcaccggcgg cttcgatctg gctcggaaag ctcggggtga ggcgatttcg cggcatccgc
     8701 accgacgcgc gctgatctca gagaaattcg gacgcgattc cgatgacacg aagcgcgcct
     8761 gcatggattc ggaattccgc cggaccgacg ccgggcgcct ccccgtcgat gtccatccag
     8821 gccgtattct cgagcatctc cacctcgatg tgccttccgc ggtacgtgct gacgatcggt
     8881 gagcggacgt gcgtcccttt gtagagcctt gccataaccg gaagcccgcg aagggtcgag
     8941 gcggcgcgaa gcaagatcac gtcgaacatg ccgtctgcga cgtgagcctc gggcgcaaac
     9001 atcatcccgc cgccggccca tcgtccgttg cagacgcaga ggttgctgat ctcgtgctcg
     9061 ccttcgtcct tgccgtcgat gcgcatacgg atgcgagcag gtttgtactt catctgcgcg
     9121 cgaagcacgg ctttgaagta ttttgccttg ccgctgcttt tcgacggtga cgccgcaacg
     9181 aatcgatcga cgagcccacc catacccatc gatgtgatgt tgagaaaaaa ccgactttcg
     9241 gtgtggccct cgtcgtggac gtattggacc cagccgatgt cgattggaac ggcgggcttc
     9301 tcgcgaatcg ccgcacagcc gctttcgatc tcttcggcgc cggggatcat ctttcggaag
     9361 tccccgcccg tgccggcatg aaggatgccg agcgtgagct ctttgttggc tccggattgc
     9421 atgatggcgt cgacgacttc gctgtgcgtg ccgtcaccac caaaagagac gatggttcga
     9481 acacctcgcg cgatcgcatc tttgccgatc cgtactccat cgccccgcga tgcagtgagc
     9541 tggagctcca cgggcccgag gccctctcga atgcgcgcac tcagctccgg ccaattggca
     9601 ccgacgaagc cgtggcgcgc catgggattg gcgatgacga gggtcttgct ggggtcgaac
     9661 atggcagagg gcctcgtacc gcatttcgga cctgtctggt agagtcgccg gatgcttagc
     9721 aggatttggg tcttcgtttt cgccctggcg tgcacgggct gcgggagcga cccggcgtcg
     9781 agcgtatgcg agccgctgac tgcaggggaa tgcgccgagc cagacgcagg ggtgccggac
     9841 ggtggttcgc cgctcgagtc gtccgtcgta gtcgatgtcg attggctgga ggcgcgcctc
     9901 ggagaccccg acgttcaact catcgataca cggagctttg ggtacgaagc ttcgcggatt
     9961 ccgggggcta tcgaattgcg cccatccgct ctcgcgaagg cgatcgacgg cgtttcgtct
    10021 cagctggttt cagccatcga ggcccagccg gtcttacgcg cggcagcact ccggaacgac
    10081 gtaacagcgc tggtctacgg cggatctcct gagtacgacg cggcccgcgt cgtctgggcg
    10141 cttcgttact acgggcacct cgatgttcgc tatctcaacg ggggattcgc tgcctgggtc
    10201 gaggctggag gtgcgctcga caccgatccg ccgaacatcg agccgtccga gtacagcatc
    10261 gtcgcggtga acgaagatct tctcgtcagg ggggactggg tgctctccca gctcggcgaa
    10321 gccccgtacg aggctccggc aattcaactc gtcgacgcgc gctcgagcgg tgaatacgaa
    10381 gccggccata tcccaagcgc tcgcagcgtc aactggtcgc agaatctcga ccgaggattg
    10441 ctacgctctc gcaccgagct cgaggcgctt tactccgggc tcgctcccga ccgaaccacg
    10501 gtcacttact gcgcctctgg cttacgaggc tcattcgcct ggctcactct aagcgcactc
    10561 ggctacagcg acgccagact ctacgacggc tcgtggaacg agtggggcgc gggcgggttc
    10621 ccggtcgagc agtagcgcgc gcagtgtcac cggaggacgg ctcgcgacgg gcgtgctcgc
    10681 gattgggacc cactcgcccc cgattcctta agacgaccta cggctaggct cgacccaatc
    10741 gctgcgctgc ccatcccgag ccgtcctcct ccgt
//
LOCUS       DASRZF010000431         9273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2398725, whole genome shotgun sequence.
ACCESSION   DASRZF010000431 DASRZF010000000
VERSION     DASRZF010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 9273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9273
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2398725"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..546
                     /locus_tag="VFG22_18950"
     CDS             190..546
                     /locus_tag="VFG22_18950"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HET6418383.1"
                     /translation="METRSVYETDDEVQALALQEALESDGIAAVLVNHRDTAYPGITD
                     RQRHGTEIRVEVDQVEMATALIEEFLAAQPVEGPPIAPPPDSSPAPGVAGPGWLVITL
                     IFLGLLGAAAYLLSGK"
     gene            605..784
                     /locus_tag="VFG22_18955"
     CDS             605..784
                     /locus_tag="VFG22_18955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418384.1"
                     /translation="MVVTETYGLRELAKLLNWNPAHCKVILKQLGADPAQPIDEDTAA
                     KVASKIRRPWPPQAA"
     gene            complement(781..2403)
                     /locus_tag="VFG22_18960"
     CDS             complement(781..2403)
                     /locus_tag="VFG22_18960"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HET6418385.1"
                     /translation="MSEYDYIVIGSGSAGAIIASRLSEDPAVTVLLLEAGGSDRSAVV
                     RKPGMISLVHQVKQLKRKVDWGFSTAPQKCLNERRIPYTRGKLLGGSSSVNGMLYLRG
                     HRRNYDDWAANGCAGWSFEDVLPFFKKLENHEDGETAYHGAGGPIQVTRHPADQISPV
                     SNAFVRAASRVCDIPISDDFNGENQNCAGMYQMSASGGVRSGTGEAYIQPSLQRPNFS
                     VEMRALVHRVVIENGRATGVEYSQPGGDQLARAKREVIVAGGAIGSPQLLMLSGIGPA
                     DHLREQGIEVELDLPGVGKNLHDHLFVPLTFRSPTSLHRGTASHFFAGMLKEYLLGNG
                     WFGRTVFEAGAFIKSDDSAPIPDIQIHTLPWGYPSPNQDGPERPYIDTGHCLTVMPTL
                     IYPKSRGEVLLASKSPDEGPQIDPHFLEEPEDLDLLLTAIRKCREICAAPELAEHLGE
                     ELSPGAQRSSKSELTEEIRLRATTVYHPVGSCKMGVDEMAVVDSKLKVRGVEGLRVAD
                     ASIMPQITGGNTNAPSMMIGERAAALIREDAS"
     gene            complement(2400..4103)
                     /gene="recD"
                     /locus_tag="VFG22_18965"
     CDS             complement(2400..4103)
                     /gene="recD"
                     /locus_tag="VFG22_18965"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HET6418386.1"
                     /translation="MLLSDYQSRGLLELLDVELARALGRITEDGRPEVELAIALTSRN
                     IRRGHSCFPIGISAAEVWPNEPSATDALPDPGGWREALRGSRLTQGGPLVLDSIGRLY
                     LRRYWQLERDIARELAARADVPPLMAGDSDWLGAALDALFPGEPTSAVRRAAENALRH
                     RISLLCGGPGTGKTTTVAAILALIIQGRLRESGEAPKVMLLAPTGKSAARLGEAVRRA
                     KSRIKAPDSILLHLPEEATTLHRALGMRRNGAGFRRNADLPLEADVIVVDEASMIDLV
                     LMRQLLDATPRESTLLIVGDPEQLSSVEAGSVLRDLVRASSETWWRGRVTELTTTYRY
                     DEKQPLGQLVAAIRNADQEAVEALLQSRSTHDLAWTPAKGLSSELDRAARHWSRALSS
                     LDPAEHFQFRARYLMLSPFRRGPIGTERLGAAIRDRLTAPRPGQPQVTPIIIEENSPE
                     LHVYNGDLAMLVDGEPPMATLQSEGEGFSSIAKARLPRFSDAFALSVHKAQGSEFDEV
                     LLVLPEEDAPLLTRELLYTAVSRARERVRIVGPMEVVRAALERRAQRYSGLVDAIGEA
                     R"
     gene            complement(4090..6576)
                     /locus_tag="VFG22_18970"
     CDS             complement(4090..6576)
                     /locus_tag="VFG22_18970"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET6418387.1"
                     /translation="MKRRREERAVLTFDDLLTIVYAPFDPGRANGTAFDRDRILETVS
                     GAYPLALIDEFQDTDSVQYGIFRAIYGEASAIYVGDPKQAIYAFRGADVFSYIGAVKD
                     VGRRVYTLTTNRRSDPGLVHAVNTLFSLRKPPFIIEGIEMEHAVPHHERPRSTLDPSM
                     EVVFLDRSQLEGGTAVSVSPIVANEIALLLESGAMIEARPVEPGDIAVLCRSNDQALA
                     VTEALRSLDIPVSLDGGSSVFETGIARELTAILEAALMPGESALLRRALLTSLLGVSP
                     YDLATMSDHDWSSWVSRFQNWNETWHRQGVVRFLEDLLRMTRAEIRIARLPAARRVLT
                     DLLHLEELLLRGERERHRDPVALMQWFRRLGENGPGESNLASEDLQRRPDTESGAVHV
                     STIHKSKGLEYGIVYCPFPWTRKFDEGAVKFHDEQGNIKIDLGSDQCKAHEARSDEEV
                     RSEGLRLLYVAVTRAKYRCTLYWGRTAGWKDAALGYLLHGSEGLDKVDEDGMRSALQQ
                     FAASSSGAVGWRSAHQERARARTGSSPTELLATRARSRHFSHAPRIASFTSLTGHDEK
                     TPGPRPIAPSPDRSASLFSDLPGGVRTGLFLHAVLEQADFQLLGDAETTLLIERQIRN
                     FGFDPALTPIVQADLQLVGGTPLTDEADAPRLIDLGRASQLRELEFTLHVDGPKLEDL
                     AGILMRHAAPAAAPRYHERLADLSAQTLQRFLRGYIDLMFEWEGRWYVADYKSNTLPN
                     YAPTEINELVQSAHYLLQAQLYTAAAQRYLRQRIADYDPETQWGGALFLFLRGMTGPT
                     EAGSSVFFDRQPAELLRAVDSWLGGVDAPL"
     gene            complement(6607..7575)
                     /locus_tag="VFG22_18975"
     CDS             complement(6607..7575)
                     /locus_tag="VFG22_18975"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET6418388.1"
                     /translation="MSSIAPQLIPFDAPSLVQASAGTGKTYTITTYFVRAILEQDLKP
                     EQILVVTYTKAATAELRVRTRERILDAIRLLDEPSGGVDVLQSVTSDAVERLGRRAAE
                     SRLRVALSQMDRAAILTIHGFCQRLLQEYPLLFGIDFDFEVSEDAATMYSELAVDFWA
                     TELYDAPEWLLRALEEERLNPRALARLASRALIPGIEILGPAPVELDPAVFERWLLLK
                     RQASELWSSDRKEIAEILASNEDLNRRSYQAQTVTSKWLPGLDDFFARTGFGFLPEFF
                     SKLATGRMKMKKGCAEPRHPFFEASALLLEAHESLAGGLNYAVFSL"
     gene            complement(7572..>9273)
                     /locus_tag="VFG22_18980"
     CDS             complement(7572..>9273)
                     /locus_tag="VFG22_18980"
                     /inference="COORDINATES: protein motif:HMM:NF037683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418389.1"
                     /translation="PFEGFLPRGAPSLSDAELLARLSRLCEVLFDFQRRSREPLSIED
                     WTRELEHFAALLFAEDDESSSAMRTLREAIADLRDVATRTGYDDPILLKTLRRELGAI
                     LQEKAPAVGFLRRGVTMTELVPLRSVPFRVVCLVGMSEESFPRADDRPSFDHSRATHQ
                     HGDRNKRDDDRHSFLQAVLCARDRLRISYSAPAHNPRRGANPSPVVWELCETLRRYYV
                     SGEGAPVLTPTMHPLHPFDARYFEPSALPRSCSERHLEIACALRQGGESPRRIELCTE
                     ADEALESLSVNELSAWLWNPMSTFVERVLRARFEATQLYEPSQALTTIGRREAALLGN
                     AALRVGLRDAALDAYLDAAPDFPDGNWGALERQRLASEIRMVDARGDALRTGHEIQST
                     LVSAELNDLLLEGRLDGIDGPQRLLKRFTKPGRRTELTIWIEHLLMQTFGELPKTTHL
                     VLRGTETRAETVSFAPVADPHAELRNLVTLYVQSRERPLPLLGESSWLFMETRNSSGS
                     GKAFSEAAKLLSSQRTWNPSLDYALGEEDPFSDSDWREAFEQAAAAVYEPLLRHRTLV
                     "
BASE COUNT         1775 a         2857 c         2892 g         1749 t
ORIGIN      
        1 tcgaccgggc tctcgagcgg tacgacgcag ccacgcccgc cctgaagcga gagatcatgc
       61 tcgcctgcgc cgcaaccgtc atggcggacg accaagtcac cgaccgggaa gccgagctcc
      121 tccgcgccat cggagacgca ctcgattgtc ccgtaccgcc tttcgtccaa tcgagctagc
      181 atgggcgtca tggagacacg aagcgtctac gaaaccgacg acgaagtaca agcgctcgcc
      241 cttcaagaag cccttgaatc cgatgggatc gcggcggtct tggtcaacca ccgagatacc
      301 gcttacccgg gcattaccga ccggcagcgg cacgggaccg agattcgtgt cgaagtcgac
      361 caagtcgaga tggcgacggc gctcatcgag gagttcctgg cggcccagcc cgttgaaggg
      421 cctcctatcg cgccgccgcc agactcgagc ccggcgcccg gagtggcagg acccggctgg
      481 ttggtaatca cactgatatt cctgggttta ttgggagccg cggcttattt actaagtggc
      541 aaataaccct tggatcaggt tccattttgc gctataaatt tacggttagt aaaggaagcg
      601 cacgatggtt gttacagaga catacggcct tagagagctc gcgaagctac tcaactggaa
      661 cccggctcac tgcaaggtga tcctcaagca gttgggtgct gacccggcgc aaccgatcga
      721 cgaggacacg gccgccaagg tggccagcaa gattcgccgc ccctggccgc cgcaggctgc
      781 ttaactcgcg tcttctcgaa tgagcgccgc agcgcgttca ccgatcatca tgctcggggc
      841 gttcgtgttt ccgccggtga tctgcggcat gatcgacgca tctgccacgc gtagcccttc
      901 gacaccgcgc accttcagtt tcgaatccac gactgccatc tcgtcgaccc ccatcttgca
      961 gctaccgacg gggtgataga ccgtcgtcgc gcgtaggcgg atctcttcgg tgagctcgct
     1021 cttgctcgag cgttgcgcgc caggactcag ctcctcgccg aggtgctctg cgagctcggg
     1081 agccgcgcag atctctcggc actttcggat cgcagttagc aacaagtcca agtcttcggg
     1141 ctcttcgaga aaatgaggat cgatctgcgg cccttcgtcg gggctcttag acgcgagcag
     1201 aacttcgcct cggctcttgg ggtaaatcag cgtcggcatc acggtcagac agtgacccgt
     1261 gtcgatgtac gggcgctcgg gaccatcttg gttcggcgat gggtaccccc atggaagcgt
     1321 atggatctgg atgtcaggaa tcggtgctga atcatcgctc ttgatgaacg cgccggcttc
     1381 gaagaccgtg cgtccgaacc agccatttcc gagcagatat tccttgagca ttccggcaaa
     1441 gaaatgtgag gcggttcctc ggtgcaggct cgtcggagac cgaaacgtga gtggcacgaa
     1501 gaggtggtca tgcaggttct tgcccacccc tggaaggtcg agctccacct cgatgccctg
     1561 ctcgcgcagg tggtcggcgg gcccaatgcc ggagagcatg aggagctgcg gggagccgat
     1621 cgcgccccca gcaacgatca cctcccgctt cgcgcgggcg agctgatctc caccgggctg
     1681 cgaatactcg acgcccgtgg cgcgaccatt ctcgatcacg actcgatgca cgagcgcgcg
     1741 catctccacg ctgaagttgg gacgttgcaa gctcggctgg atgtacgctt cgccggttcc
     1801 gcttcggacg ccgccgctgg cggacatctg gtacattcca gcgcagttct gattctcgcc
     1861 gttgaagtcg tcactgatcg ggatatcgca aactcgagat gcggctctca cgaaggcgtt
     1921 ggagacaggg cttatctgat cggcagggtg gcgcgtgact tggatcggac cgcccgcgcc
     1981 atggtatgcg gtctctccat cttcgtggtt ctcgagcttc ttgaaaaatg gaagaacgtc
     2041 ttcgaagctc caccccgcgc agccatttgc agcccaatcg tcgtagttcc gtcggtgacc
     2101 ccgcaggtag agcattccgt tcacggaact agagccgccc agcaactttc cgcgcgtgta
     2161 cggaatgcga cgctcgttca agcacttctg tggcgcggtg ctgaagcccc agtcgacctt
     2221 tctcttcagt tgcttgacct ggtgcacgag gctgatcatc ccgggcttgc gcaccacggc
     2281 gctgcggtcg cttccgccgg cctccagtag caagacggtc accgctggat cttcggagag
     2341 acggcttgcg atgatcgctc cggcggaacc agagccaatg acgatgtagt cgtactcgct
     2401 catcttgcct ctccaattgc atccacgagc ccagaatagc gctgagctct tcgttcgagg
     2461 gccgctctga cgacctccat ggggccgacg atccgtaccc gctctcgggc tcggctcacc
     2521 gccgtgtaaa gcaactcgcg cgtcaggagg ggcgcatcct cttcggggag tacgaggagg
     2581 acctcatcga actccgagcc ctgcgccttg tgaacgctaa gcgcgaaggc atcggagaaa
     2641 cgtggcagtc gcgcctttgc aatggaagaa aaaccttcac cttcgctctg cagcgtcgcc
     2701 atcggcggct cgccatcgac tagcattgca aggtccccat tgtacacatg aagctcggga
     2761 ctgttctctt cgatgatgat cggtgtcact tggggctggc cgggcctcgg agccgtgagg
     2821 cggtctcgaa tcgcagcgcc cagtctctcg gtcccgatcg gtcctctgcg gaacgggctg
     2881 agcatcaggt atcgagctcg gaactggaaa tgctctgcag ggtccaagct cgaaagcgct
     2941 ctcgaccagt gacgagcagc gcgatcgagc tccgagctca agcccttggc cggagtccac
     3001 gcaagatcat gggtgcttcg actttggagt aacgcctcca cggcctcctg gtcagcgttc
     3061 cgaatcgcgg cgacgagctg gccgaggggc tgcttctcat cgtatcgata cgtcgtcgtg
     3121 agctctgtga cacgacctcg ccaccaagtt tcgctgctcg ccctcaccaa gtcacgaaga
     3181 acggagccgg cctccacgga gctgagctgc tcggggtcgc ccacgatcaa gagagtcgat
     3241 tcgcgcggag tcgcgtcgag caactgacgc atcaagacga gatcgatcat ggaggcctcg
     3301 tcgacgacga tgacgtctgc ttcgagcggc agatcggcat tacgccggaa gcctgcgccg
     3361 ttgcgccgca tcccgagcgc acgatgcaac gtcgttgcct cctccgggag atgcagcagg
     3421 atggagtcgg gtgctttgat gcgactcttc gcgcggcgca ccgcctcgcc gaggcgtgca
     3481 gcagacttcc ccgtcggggc gagcagcatc actttgggcg cctctccgga ttcacgaagc
     3541 ctcccttgga tgatcaacgc gagaattgcg gcaactgtgg tcgtcttgcc ggtgcccggt
     3601 ccgccgcaga gaagagaaat gcgatgccgc agggcgttct cagcggcccg gcgcaccgct
     3661 gaggtcggtt cacccgggaa caacgcgtcg agggccgcgc cgagccaatc gctgtcaccg
     3721 gccatcagcg gcggaacgtc agctcgcgcc gccagctccc gggcgatgtc acgctcgagc
     3781 tgccagtatc gccgcaggta gagacgaccg atcgaatcga ggaccagcgg gccgccttgg
     3841 gtcagccgac ttcctcgcag tgcttcccgc catccgcccg ggtccggaag cgcatcggtc
     3901 gcagaaggct cgttcggcca gacctccgca gctgagatcc cgatcggaaa gcaactgtgg
     3961 cctcgccgga tgttgcggct ggtgagcgcg atggcgagct cgacttccgg tctcccgtct
     4021 tccgtgatcc gaccgagggc gcgtgccagc tccacatcga gaagctcgag gagaccccgc
     4081 gattggtagt cagagaggag catcgacccc cccgagccag ctgtctacag cacgcaagag
     4141 ctccgctggt tggcgatcga aaaaaacgct cgacccggcc tctgtcggtc ccgtcattcc
     4201 gcgcaagaac agaaagagag cgccgcccca ttgcgtttcg ggatcgtagt cggcgattcg
     4261 ttgcctgagg taacgctggg cagccgcagt gtaaagctgg gcctggagca ggtagtgcgc
     4321 gctctgcacc aactcgttga tctcagtcgg cgcgtagttt ggcagcgtgt tggacttgta
     4381 gtctgcgacg taccagcgac cttcccactc gaacatcaaa tcgatgtacc cgcgcaagaa
     4441 gcgctggagc gtttgtgcgc tgagatctgc aaggcgctcg tgataccgcg gtgcggccgc
     4501 aggcgcggca tgccgcatca agattccggc gagatcttcg agcttgggac cgtccacgtg
     4561 cagcgtgaac tcgagctcgc gaagttggga ggcccgaccg agatcgatca ggcgtggcgc
     4621 atcggcctcg tcggtaagcg gcgtcccgcc gaccaattgg agatctgctt gaacgatggg
     4681 tgtgagtgcg gggtcgaacc cgaaattccg aatctgtcgt tcgatgagca gcgtggtttc
     4741 ggcgtcaccg aggagttgaa aatctgcctg ttccagaacc gcgtgaagaa acaatcccgt
     4801 gcgtacgccg ccgggcagat ccgagaagag agaggcgctt cgatcaggcg acggcgcgat
     4861 cggtcgcggg ccgggggttt tctcgtcgtg tccggtcagg ctcgtgaagc tcgcgatgcg
     4921 tggggcgtga ctgaagtgcc gcgaacgcgc gcgcgtggcg aggagctcag taggtgatga
     4981 gcccgtgcgc gctctggctc gttcctgatg tgcgctgcgc cagccaacgg cacctgatga
     5041 cgaggcggcg aattgctgga gggcgctgcg cattccgtct tcgtcgacct tgtccaggcc
     5101 ctcggaaccg tggagcaggt agccaagcgc agcatccttc cagccggcgg tgcggcccca
     5161 ataaagtgtg cagcgatact tcgcccgcgt aacggcgacg tagagcaaac gcagtccttc
     5221 cgagcgtact tcctcatcgc tgcgcgcctc gtgtgccttg cactgatccg agccgaggtc
     5281 gattttgata tttccctgct cgtcgtggaa cttcaccgcg ccttcatcaa acttgcgagt
     5341 ccaggggaac gggcagtaca cgatgccgta ctccaggccc ttgctcttgt gaatcgtgga
     5401 gacgtggaca gcgccggatt ccgtatcggg acgccgttga agatcttcgg aggccagatt
     5461 gctttcaccg ggtccgtttt cgccgagccg tcgaaaccac tgcatgagcg ccaccgggtc
     5521 gcggtgacgc tcccgttcac cgcggagcag cagctcttcg agatggagta gatcggtcag
     5581 gacgcggcgc gcggccggaa gccgagcgat gcgaatctcc gccctcgtca tacgcagaag
     5641 atcctcgagg aagcgtacga cgccttggcg atgccaggtc tcgttccagt tttggaatcg
     5701 ggacacccag ctcgaccaat cgtggtcgga catcgttgcg agatcgtacg gggacacgcc
     5761 gagcagcgag gtgagaagcg cacgacgcag gagggcagat tcgcctggca tcagcgccgc
     5821 ttcgagtatg gctgtgagct ccctggcaat accggtctcg aagacgctcg agcctccatc
     5881 gagggacacc ggtatatcga gcgacctgag cgcttcggtg actgcaagtg cctggtcgtt
     5941 gctgcggcag agcacggcga tgtccccggg ctcgaccggt cgggcctcga tcatcgctcc
     6001 cgactccagc agcaacgcaa tttcgtttgc gacgatggga gatacggaaa ccgccgttcc
     6061 tccctcgagc tgagagcgat ccaagaacac gacctccatc gatgggtcga gggtcgaccg
     6121 ggggcgctca tggtgtggca ccgcgtgttc catctcgatc ccctcgatga tgaagggcgg
     6181 cttccgaaga gaaaatagcg tattcacagc atggaccaga ccgggatcgg atcgacgatt
     6241 cgtcgtgagc gtgtagacac gacgcccgac gtccttcacc gcaccgatgt atgagaacac
     6301 gtcagcgccg cgaaacgcat agattgcctg cttcggatct cccacgtaga tggccgacgc
     6361 ctcgccgtag atggcgcgaa agatcccgta ttgaacactg tcggtgtcct gaaactcgtc
     6421 gatgagggcc agagggtacg cacccgatac cgtttcgaga atacgatctc gatcgaaagc
     6481 ggtgccattg gctcgaccgg gatcgaaggg cgcgtagacg atcgtcagca agtcgtcgaa
     6541 cgtgaggacg gctcgttcct ctcgccgccg tttcacctgt tcgcgcgcga actcgatgaa
     6601 ccgtcgttac aaggagaaga ccgcatagtt caaccctcct gccagggatt cgtgggcctc
     6661 gagaagcagc gctgaggctt cgaagaatgg gtggcgcggc tccgcgcagc ccttcttcat
     6721 cttcatcctg cccgtcgcga gcttcgagaa aaactccggc aaaaaaccaa aaccagtcct
     6781 tgcgaagaaa tcgtcgaggc caggcagcca cttgctcgtc acggtctgcg cctggtagga
     6841 gcgtcggttg agatcctcgt tgctcgcgag gatctcggcg atctccttgc gatccgaaga
     6901 ccagagctcg gaagcctgcc gcttcagcag caaccagcgc tcgaagaccg cggggtcgag
     6961 ctcgactggt gcgggcccaa gaatctcgat ccccgggatc aatgcgcggc tcgccaagcg
     7021 cgccaaggct ctggggttga gcctttcttc ttcgagggcc cgcagcagcc attcaggtgc
     7081 gtcgtagagc tcggtggccc aaaaatcgac cgcaagctcc gagtacatcg ttgcggcatc
     7141 ctcggagact tcgaagtcga agtcgatccc gaacaagagt ggatattctt gtagcaggcg
     7201 ctgacagaac ccatggatcg tcagaatcgc cgcgcgatcc atctgcgaga gtgcaaccct
     7261 caaacggctc tccgcagcac gccgtccgag gcgctcgacg gcgtcggaag tcacggactg
     7321 gagaacgtcc actccaccgg aaggctcgtc cagcaaccgg attgcgtcga gaattcgctc
     7381 gcgcgttcgc accctgagct ccgcggtagc cgccttcgtg taggtgacca ccaggatttg
     7441 ctccggcttg agatcctgct ccaagattgc ccggacgaag tacgtcgtta tcgtgtaggt
     7501 cttgccggtt cccgcgctcg cctgaaccaa ggacggagcg tcgaacggga tcagctgcgg
     7561 agcgatggag ctcataccaa agtccggtgt cgaagaaggg gttcatagac cgcggccgca
     7621 gcctgctcga aggcctcacg ccagtccgaa tcggagaacg ggtcctcctc tccgagcgcg
     7681 tagtcgaggg aaggattcca agtgcgctgg ctgctcagca gcttggccgc ctcggaaaat
     7741 gccttccccg agcctgacga gttacgcgtt tccatgaaga gccaggacga ttcgccgagc
     7801 aatggaagcg gccgctcgcg ggactggacg tagagcgtca ccaggtttct cagctcggcg
     7861 tgcggatcgg cgacaggagc aaaagacacg gtctccgccc tcgtttcggt tccccgaagc
     7921 accaagtgcg tggtctttgg aagctcgcca aaggtttgca tgagcagatg ctctatccag
     7981 atcgtgagct ccgtacgtcg tccgggtttg gtgaaccgtt tcagcagtcg ctgtggcccg
     8041 tcgatgccgt cgagccgccc ttcgaggagc aagtcgttca gctccgcgga gacgagagtt
     8101 gattggattt cgtggccggt tcgaagcgcg tcccctcgtg cgtccaccat ccgaatctcg
     8161 cttgcgagac gttgccgctc tagagcaccc caattgccgt cggggaagtc gggcgcggca
     8221 tcgaggtatg catccaacgc agcgtcgcga agtccgacgc gaagcgcggc gttgcccagc
     8281 agagcagcct cgcgtcggcc aattgtggtg agcgcctgac tgggctcgta gagttgtgtc
     8341 gcttcgaatc gagcacgaag cactctttcg acgaaggtgc tcatagggtt ccatagccaa
     8401 gcggagagct cgttcacgga cagactttcg agggcctcat cggcctccgt gcaaagctcg
     8461 attcggcgtg gtgattcgcc tccctggcgg agagcgcagg caatctccaa atgccgctcg
     8521 gagcagctcc gaggcaaggc acttggctcg aaatacctcg catcgaaggg atgcagcggg
     8581 tgcatggtag gcgtgagcac cggtgcgcct tcgcctgaga catagtagcg acgcagcgtt
     8641 tcacaaagct cccagactac gggggaagga tttgcacccc gtcttggatt gtgggccggc
     8701 gcgctgtagc tgattctcag ccggtcgcgg gcacaaagaa cggcctgcaa gaacgaatga
     8761 cgatcgtcgt cccgcttgtt gcgatcgccg tgttggtgag tggctcggct gtgatcgaag
     8821 ctcggacgat cgtccgctct ggggaagctc tcctcgctca tgccgacgag gcaaacgacc
     8881 cgaaaaggaa cggaacgaag gggtacgagc tcggtcatcg taacgccccg gcgaaggaac
     8941 cccaccgcgg gcgccttctc ctggagaatg gcgccgagct ctcttcgcaa tgtcttcagc
     9001 aaaatcgggt cgtcgtaccc agtccgcgtc gcgacatctc gaagatctgc gatcgcctct
     9061 cggagcgttc gcattgcgga gctgctttcg tcatcctccg cgaagagcag ggcggcgaag
     9121 tgttcgagct cgcgagtcca atcttcgatg ctgagcggct ctcggctgcg acgctggaag
     9181 tcgaatagga cctcgcacaa ccgtgaaagc cgggccagca gctcggcgtc gctgagactc
     9241 ggagcaccgc ggggcagaaa gccttcaaaa ggg
//
LOCUS       DASRZF010000432        15416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2399601, whole genome shotgun sequence.
ACCESSION   DASRZF010000432 DASRZF010000000
VERSION     DASRZF010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 15416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15416
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2399601"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VFG22_18985"
     CDS             complement(<1..197)
                     /locus_tag="VFG22_18985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HET6418390.1"
                     /translation="MSDPISKELFARACKVIPGGVNSPVRAFRAVGGEPIFIKEAHGP
                     ILVGADGTEYVDYIGSWGPMIL"
     gene            complement(531..1286)
                     /locus_tag="VFG22_18990"
     CDS             complement(531..1286)
                     /locus_tag="VFG22_18990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HET6418391.1"
                     /translation="MQYHSVGLTDVGQRRKLNEDAFLVDDGLGLYVVADGMGGHAAGE
                     IASQEAVDTVHGMVSRGHEALDRVAQGDTSEESLQRAIRTVESAVQAATYMVFGLAQH
                     DPEQQGMGTTVSVLAMCGDYGITAQVGDSRIYLIRGHAAEPLTEDHTLVAWQLKQGII
                     TEAEAARSPHRNVITRAVGSREYVQVDTGFFEVQPSDAFLLCSDGLHGYLQPHEIPDV
                     IDMGPNGAAQRFIQMANSRGGRDNITAVVVTVR"
     gene            1440..2816
                     /locus_tag="VFG22_18995"
     CDS             1440..2816
                     /locus_tag="VFG22_18995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6418392.1"
                     /translation="MKHGEATQDLQSAWEALLRRQSQLASDRTLAELALGLIRELGNG
                     PAHREVLHRLAAAWTSDWQITLAAASMLIEQAGRRGMDEPPLRQDGPATWAADALQSS
                     LNALDENERANADIAGSLYATLGNALRLSGPNRDSEAQEAFTRAIELDPERGEWWYDA
                     GLLDKWRGRFDDGYAANEQARMRMGAERPVLWNLAICATALGKAEEAVEIWDELGVPA
                     RIDVARGMPYVAGLPPMLLRVLSRTPATDGTSELPEKAVGFELVWVAPLSPCHGVVQS
                     PTFRDAPIDYGDLVLWDGAPVAAHKTSEGDTVPVFPLLEILRRGDERRWPFVALEREP
                     GSVRSLEADLPEGARVFIQQERVEHHCAACEAGEPHGHDTIPATQTTPAFESAAGTLV
                     RGKLIVPEAQDLSKLRSAWEAAAGSRPLSISLPALYEELGDSKQAGQAHQAWRGIEGK
                     VLRRKAQA"
     gene            2813..3616
                     /locus_tag="VFG22_19000"
     CDS             2813..3616
                     /locus_tag="VFG22_19000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HET6418393.1"
                     /translation="MSQLAVLPSEQYEFEGLPRLDEAKIQERRQLVFLVLSGLFLGTL
                     AMLNILGVTRFIDLSFQLPGTQITVPMPLAVGVLPYPITFLCTDFISELFGRHRANQV
                     VWTGLALNLWVMFILWIGGALPGFEPAGQPETGVFFELRRLAFSAVTASMIAYLAAQF
                     VDVQIFHFWKDFTKGRHLWLRNNGSTLVSQLVDTVAVILITHFLANGIPIEAGKPLWP
                     QLFTFIAAGYTFKLTVALLDTIPFYLGATHLGRYLRIHPVHGTRPGSVS"
     gene            3744..4907
                     /locus_tag="VFG22_19005"
     CDS             3744..4907
                     /locus_tag="VFG22_19005"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6418394.1"
                     /translation="MSQTGVRLGKYQLVRRLDVAEAGEAHLAKMIGIEGFEKQVVLWT
                     FRAGPEHAPALIAKVTMEAKRAASLSHANIAQVLDLDVIDGQCVLTTEYVPGRTLEAV
                     LRESGRLPWSIAARVACEVAAALSYAHSQRTVDGELLGVVHRRLSPSRIVLGDHGDVK
                     VTGFGTSSAWTTSNAYPAPEEVRGEPVDGRADVFALGISLRESLRSTGVPEPLLRILG
                     RALNEYPEHRPSAAELGQDLSCVLRGADGPATHRDIAAFARGRPKLAAARAERSFAEY
                     LVRTSTALQMRGALFEALDRLELALASISTRGSSEAETALDLYERIGRLSVEAQVGDR
                     GIHQMTRALDLADSLGRDRQAALFCALRGELLAQAQRKDESRDWLERAAMLRR"
     gene            complement(4912..5319)
                     /gene="secG"
                     /locus_tag="VFG22_19010"
     CDS             complement(4912..5319)
                     /gene="secG"
                     /locus_tag="VFG22_19010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HET6418395.1"
                     /translation="MYTFISIVYVFVCLFLILVVLLQSGKGGGLGSAFGGGASQQIFG
                     GAGAGNLLTRLTAGFAFTFMALSVWLAFLSSSGEQALDRAVREQAAAQEVAAEVEEEE
                     LEEAVVGAEVEAEIEAELEAEEAAEETETPDSK"
     gene            complement(5324..6100)
                     /gene="tpiA"
                     /locus_tag="VFG22_19015"
     CDS             complement(5324..6100)
                     /gene="tpiA"
                     /locus_tag="VFG22_19015"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HET6418396.1"
                     /translation="MTKRKPLIAGNWKLHNTIAESQALAGAIAAHVGTGLACDVVVAP
                     VATSIAAVRESVRVSVVGLSAQNTHWEDSGAWTGELSPALLVDAGCGYCIIGHSERRQ
                     FFGETDAGVRKKVSALLVHKVVPILCVGESLEQREAGETLKVVLGQVEAATEGLDAVA
                     LAPLVVAYEPIWAIGTGRTAKAEDAQEVHLAIRGHLSALKGRSWADSVRILYGGSVKP
                     SNAHELLSQPDVDGALVGGASLSTDNFIPIIDAGVRRAES"
     gene            complement(6097..7272)
                     /locus_tag="VFG22_19020"
     CDS             complement(6097..7272)
                     /locus_tag="VFG22_19020"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HET6418397.1"
                     /translation="MRSIEDLSLESVTTFIRVDFNVPIKDGEIGDDTRIRAALPTIRY
                     ALEQGARVVLASHLGRPKGQVVPGLSLEPVAVRLAELLEDGEVRLTDDCIGDGARKVV
                     QDLRDGQVALLENLRFHPEEKSNDVDFARELGSGCTAYVNDAFGAAHRAHASVSALPG
                     LIEERAAGFLMQRELEALDRLRTDAPRPYIAILGGAKVSDKIGVMEALLTKVDALLIG
                     GAMANTFLAARGFDVGKSLCERDKLALARSLMAAAEEKRVDLLLPIDVRVGESTESTE
                     ARVTGVDAIPADSMALDIGPATERLYRERLRRALAVFWNGPMGLFENAAFAEGTLAVA
                     RAAADCPGFSVVGGGDSVAAVKQANLANRFDHVSTGGGASLEYLEGRSLPGVEALKP"
     gene            complement(7289..8299)
                     /gene="gap"
                     /locus_tag="VFG22_19025"
     CDS             complement(7289..8299)
                     /gene="gap"
                     /locus_tag="VFG22_19025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HET6418398.1"
                     /translation="MATKVGINGFGRIGRCVARIVAERGGDVEIVGINDLTSADMLAH
                     LLEWDSVHGHFSHPVKAEADAIWIGDRRIPVSAERDPAKLKWKELGAEVVLECTGLFR
                     TREKAAAHLAAGAERVIISAPGKGEIDGTFVVGINSDKFDPAKDHVISCASCTTNCLA
                     PLVKVLNDSFGIVKGHMVTVHSYTNDQGLLDQPHSDKRRARAAAVSMIPTSTGAATAI
                     GLVLPELKGKLDGTAIRVPTPNVSLTCLTAQVGRNTDREEVNRALKAAAEGPLKGIFY
                     FEERPLVSQDFMGNPHSSICDAAQTQVVGGDLVEVQSWYDNEWGFSNRMVDLVRIVSS
                     AD"
     gene            complement(8443..8595)
                     /locus_tag="VFG22_19030"
     CDS             complement(8443..8595)
                     /locus_tag="VFG22_19030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418399.1"
                     /translation="MDNRGIRIIAKSVYRELRNSGHSRSDIVAFTNAVLELVTTELRD
                     AQEAAE"
     gene            complement(8845..9471)
                     /locus_tag="VFG22_19035"
     CDS             complement(8845..9471)
                     /locus_tag="VFG22_19035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6418400.1"
                     /translation="MQGSEFIAAHEKMVHGLAHRLRRELSLSGELEDLIAFGFGGLLE
                     AHHRFDPGRGVQFQTFAYHRVRGAMLDGVRKMAQLPRRAHERLQASSEKVPTAAPGAF
                     DRVFMQMSAGLTTGTVLQGRFAEDSPEALLLKNESISRLLAALPRLSPRQRILVRGFY
                     FEGRSIDSMARELGISKSWASRLHTSALRSLREAIESSEAKSGRPAEI"
     gene            9587..10144
                     /locus_tag="VFG22_19040"
     CDS             9587..10144
                     /locus_tag="VFG22_19040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418401.1"
                     /translation="MTESRPGIASANRPRLGPFASLLNEVPHDQSALDALAFAYAELA
                     EAERGALVQAVVQDSNDPAQALLALIAVEEEPTLQRRIAGLIQRHSRIEKSAILEGTE
                     TEGESRLVQSRQGLGSESLRIAWKLSKISVIEVEPRTLSKIDGHLVPVATAVDRLLPL
                     LWRHIRAGGRLPEGAERFAGFFSAV"
     gene            complement(10146..13247)
                     /locus_tag="VFG22_19045"
     CDS             complement(10146..13247)
                     /locus_tag="VFG22_19045"
                     /inference="COORDINATES: protein motif:HMM:TIGR03900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5808 family serine peptidase"
                     /protein_id="HET6418402.1"
                     /translation="MTRIWKPLLGLVVVLAAFTVSFGWPVENGLQIDIDTSPRAQAAR
                     EKTPWDLKKLQVMNRVIHQVNEDYVDPKRVDHQKMMLAGLDAIQRDVAPVLVYYEDGA
                     DTLRIQVNSAESEFAVKDVTSPWALSGRFRDVFSFLQEQLKNEEDIELNEVEYTAVNG
                     MLRTLDPHTVLLPPEHFKEMQTSTRGEFGGLGIVISIRDGHLTVIRPIENTPASRAGL
                     QRNDRIVKIEDESTLNMPLQEAVNRLRGKPGSKVTIFVRRQSKAGDWAAPRRVPLTRE
                     NIPVPSIEHRMLRDGVGYIRIKNFQGNTTDDLRQALGSLHKKRMVGLVLDLRDNPGGL
                     LDQAVQVADTFLEEGPIVTTSSQDPRERTEEYAERAGTEPDYPIVLLINSQSASASEI
                     VAGALKNHDRALIIGETSFGKGSVQVLYPLQDSSALKLTVAQYLTPGDVSIQGVGIVP
                     DIAINPMTVDQEVMDLKVDALFRREVDLEAHLTHASATDSQKPAVVMGYYLNADLRKR
                     LMEAEPGEEENEDQEAFLIRFSRSLVLNAKSPSRPQLLRSAKPIIERVQQQETEKVAR
                     DLKKLGIDWSVGADNGPSKVRVKATTSSGDQPTRAGDPLSLRVEVTNEGGQTLYQLAA
                     ATRSDNPWFDRRELVFGKLAPGETRSWTTTLGNCTTEDEKRVCRVPRSMMDRADAIVV
                     EFEEAHGHVPEPAEVRTVIRALPEPRFAYGLQIADNLNGNGDGRVQPGEEVTLYFTVK
                     NVSDVDSFEVQANVQNKSGAGVLLHDGRFRRDSIPAGESWVVPFTFQVLPDFKQTEAA
                     LALSVIDTEIGTGTGQKIKVPIYREDLAVRALPAKRVVSLEAGTEVREWPDSNAPLVA
                     IVQKPTRLLHTASSGSFERVNLGGGRPGWVAAAAIAPAQGQVAQEPGLEWVTVNASPE
                     IDLAPLGTFVTSEDAVRIQGKATDGQRVRDLYISTSRHKVYYESNQGSANPKELDFDA
                     EVPLHPGMNSIMVVAREDNDSVSRHYFMVRRDAPDGSLLETTQFEGALLSNGGGHHSA
                     PER"
     gene            13345..14715
                     /locus_tag="VFG22_19050"
     CDS             13345..14715
                     /locus_tag="VFG22_19050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET6418403.1"
                     /translation="MPHRILRQQIAMPVLEICKELRNAGERAWVVGGCVRDTLRGQNV
                     NDWDVATTALPEKVQKTFRRVIPTGVDHGTVTVLWKGGAYEITTLRGEGAYSDGRRPD
                     NVVFVEDIDRDLARRDFTVNAIAYDPVDGRLADPFNGLDDMRGKVLRAVGNPNERFRE
                     DGLRILRGARFVATLDFELEEDTEAAFRGALDTFRKVSPERVREEWLKTMKAPVPSRA
                     FEVMRRTGILEVTYPELLEQVGCEQNQWHAYDVWGHTMRVLDESAADPVERIAALLHD
                     VAKPRTRAKSEKTGDWTFYHHEKVGADMAERWLRDYRFSNQERELVVSLIRHHLICYS
                     SEWTDAAVRRFIKRVGADRVEPLLRLGKADALGKGRHVDEELAALKELRTRIEQSIEE
                     GDALSTQDLAVGGQDVIEHLEGGAGPRVGEILRALLDRVIEDPSLNTRDKLMPLVEKL
                     AAKPSE"
     gene            14794..15228
                     /locus_tag="VFG22_19055"
     CDS             14794..15228
                     /locus_tag="VFG22_19055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418404.1"
                     /translation="MKLWAATVLTIVLFLPATGQAKKTEDFRHSYDQVWGAAVRLIRV
                     DQGYPIKDRDETVGYFLFDYRDDGRLYPGSVELVRIQDQGGGPIRVVVQIPAMPSYIE
                     RMLLDKLKRKLLDEYGEPAPPPPPPDPTPEEPAEPPSSEPDN"
BASE COUNT         2837 a         4857 c         4812 g         2910 t
ORIGIN      
        1 aggatcatcg ggccccagga gccgatgtag tcgacgtact cggtcccatc ggcccccacc
       61 aggatcggcc cgtgggcctc cttgatgaag atcggctcgc ctccgaccgc gcgaaaggcg
      121 cgaacgggcg agttgacgcc accggggatg accttgcacg cgcgagcgaa gagctctttg
      181 gagataggat cggacatgct gcggagcgtt cagattcatc gagcccctgt caagcaggat
      241 tggcgctgac actggcgctg gcgctggcgc tggcgctgac actgacactg gcactggcac
      301 tggcactggc gctggcactg gcactggcac tggcactggc actggcactg ggagtgtatg
      361 gagcaggacg gctcgggatg ggcagcacag cgattgggtc gagccgggcc gtaggtcgtc
      421 ttatggaatt gggggcgagt gggtcccaat cgcgagcacg cccgtcgcga gccgtcctcc
      481 gctgacgcag ccacgcaaca cggtcgctgg ctctgacgcc gaccgcggcg tcagcgcacg
      541 gtgacgacga ccgcagtgat gttgtcgcgg ccgcctcggg aattcgccat ctgaatgaaa
      601 cgctgcgcgg caccattcgg acccatgtcg atcacatccg ggatttcgtg tggctgcaaa
      661 tagccgtgaa ggccgtccga gcagagcaga aacgcgtccg acggctgaac ctcgaaaaag
      721 cccgtgtcga cctgcacgta ttcacggctt cctacggcgc gcgtgatcac gttgcggtgc
      781 ggcgatcgag ctgcctcggc ttcggtgatg atcccctgct tgagctgcca ggcgacgagt
      841 gtgtgatcct cggtgagcgg ctctgcggcg tgaccgcgta tcaaatagat ccggctgtct
      901 cccacctggg cggtgatgcc gtagtccccg cacatcgcaa gcaccgacac ggtcgtgccc
      961 atgccctgct gttcggggtc gtgctgtgcc aaaccgaaaa ccatgtaggt cgctgcctgc
     1021 actgcgctct ccacggtccg gatcgccctt tgaagcgatt cctcgctggt atcgccctgg
     1081 gcgactcgat cgagggcctc gtgcccgcga ctgaccatgc cgtgcacagt gtccacagct
     1141 tcctggctcg ctatctcacc agccgcgtgc ccgcccatcc cgtccgcaac gacgtacagc
     1201 cctaagccat cgtcgacgag aaacgcatct tcgttgagct tccgcctctg tccgacgtcg
     1261 gtcagtccga cactgtggta ttgcaagtca tcctcgttca gcagtggcgg ccgccgaacg
     1321 gcgctcgccc tcccaggtta gcgaaaaagg ctcccctcca ccaagaagag gtcggatttt
     1381 gcacttcacc agctccgcca gcgctgacaa gggaggggcg actgctaagc tccggccaca
     1441 tgaagcacgg cgaagcgacc caagatctcc aatcagcgtg ggaggcgctc cttcggaggc
     1501 agagccaatt ggcctcggac cgaaccctcg cagagctcgc actcggcctg atccgggagc
     1561 tgggcaatgg gccggcccat cgcgaggtct tacatcggct cgccgcggca tggacttctg
     1621 actggcagat cacactggcc gctgcctcca tgctgatcga acaggcaggc cggcgcggca
     1681 tggacgaacc gccgctcaga caagacgggc cggctacttg ggcggcggac gcgttgcaat
     1741 cgagcttgaa cgccctcgat gagaacgaac gcgcgaacgc cgacatcgcc gggagcttgt
     1801 atgccacgct cggcaacgcg ctgcgcctca gtggaccgaa tcgcgactcc gaagcgcaag
     1861 aagcattcac gcgcgcgatc gagctcgacc ccgaacgagg cgaatggtgg tacgacgcgg
     1921 gactgctcga caagtggcgg ggcaggtttg acgatgggta cgcggcaaac gaacaggcac
     1981 gcatgcggat gggcgcagag cgaccggtcc tgtggaatct cgccatctgc gcaaccgcgc
     2041 tcgggaaggc cgaggaggcc gtagagatct gggatgagct cggcgtcccg gcacgcatcg
     2101 atgtagctcg tggcatgccg tacgtcgcag gccttccgcc catgcttctc cgggttctct
     2161 cccggacccc tgcaaccgac ggcacctccg agctgcccga gaaggccgtc ggcttcgagc
     2221 tggtctgggt cgcgcctctg agtccctgtc atggcgtagt gcagtcacct acgttccgcg
     2281 acgcgccgat cgactatggc gacctggtgc tctgggatgg ggctccggtg gcggcacaca
     2341 agacctcgga aggggacacg gtccccgtct tcccgctctt ggagatcctg cggcgcggcg
     2401 atgagcgccg ttggcccttc gtcgctctcg agcgggagcc cggcagcgtg cgcagtctcg
     2461 aggccgatct ccccgaagga gctcgcgtgt tcattcagca agaacgcgtg gaacaccact
     2521 gcgccgcttg cgaggcggga gagccgcacg gtcacgacac gatacctgcg acgcagacga
     2581 cgccagcctt cgagtcggcc gccgggacgc tggttcgcgg gaagctgatc gtgcctgagg
     2641 ctcaggacct ctcgaagctt cgctcggcct gggaggccgc cgcgggctcc cgacccttgt
     2701 cgatttcgct gcccgccctc tacgaagagc ttggcgactc gaagcaagct gggcaggcgc
     2761 accaggcctg gcggggcatc gaagggaagg tgctgcgtcg gaaggcgcaa gcgtgagcca
     2821 gctcgcagtt ctaccttcgg agcaatacga gttcgaaggg cttcctagac tcgacgaagc
     2881 caagatccaa gaacgacgtc agctcgtctt cctcgtcttg tcgggactgt tcctcggaac
     2941 gctcgcgatg ctcaacatcc tcggtgtcac acgcttcatc gacctcagct ttcagcttcc
     3001 gggaacacaa atcaccgttc cgatgccgct tgccgtcggt gtcttgccgt atccgatcac
     3061 gttcctctgt accgatttca tctcggagct cttcggacgc catcgggcga accaggtggt
     3121 ttggaccggt ctcgccctca acctctgggt gatgttcatc ctatggattg gaggcgctct
     3181 gccaggtttc gagcctgcgg gtcagcccga gaccggtgtc tttttcgagc ttcgccgcct
     3241 cgcgttcagc gccgtgacgg catcgatgat cgcgtacctc gcggctcagt tcgtggacgt
     3301 tcagatcttt cacttctgga aagacttcac caagggccgc catctctggc ttcgcaacaa
     3361 tggttcgacg ctcgtgagtc agctcgtcga taccgtcgcc gtgattctaa tcacgcattt
     3421 tctcgccaac ggcatcccaa tagaagcagg aaagcccctc tggccgcagc tcttcacctt
     3481 catcgccgca ggctacactt tcaagctgac ggtggcgttg ctcgacacga ttccgttcta
     3541 cctcggcgcg acgcacctcg gtcggtatct gcgcattcat ccagttcacg gaacccgtcc
     3601 gggttccgtg agctaggcgt tcgaccagtg atcccggagt ttgtgtgcct cgcggcccgt
     3661 tccggcgtgc aggctcacac agcacagcac atgaagttga cataaaatgc gacgccttgg
     3721 gacttctgtt ggaggattcg cggatgtccc aaaccggggt tcgactcggc aagtaccaac
     3781 tcgtccgccg tctcgacgtt gccgaggcgg gcgaggcgca tcttgcgaag atgatcggca
     3841 tcgaagggtt tgaaaaacaa gtcgtgctct ggacgtttcg agcgggcccc gagcatgctc
     3901 cggcactcat cgcaaaagtg acgatggaag ccaagcgggc tgcgtctcta tcccatgcaa
     3961 acatcgctca agtgctcgat ctcgacgtca tcgatgggca atgtgttctc acgaccgagt
     4021 acgtccctgg ccgcacgctc gaggcggtcc tccgcgagtc gggaaggctg ccctggtcca
     4081 tcgcggcgcg agtcgcctgc gaagtggccg cggctctgag ctacgcgcat tcccaacgaa
     4141 cggtggatgg cgagctcctc ggtgtcgtgc atcgaaggct ctcgccaagt cgaatcgtgc
     4201 tcggcgacca cggtgacgtg aaagtcactg gcttcgggac ctcgagtgcc tggaccacct
     4261 cgaatgcgta tccggcacct gaggaggttc gcggcgagcc cgtagacggc cgcgcggacg
     4321 tcttcgcgct cggcatcagc ttacgcgagt ccctgcgttc cacgggggta cccgagccgc
     4381 ttctccggat ccttgggcgc gcgctgaacg aatacccgga gcaccgaccg agcgccgctg
     4441 agctcggtca ggacctcagc tgcgtccttc gaggggccga cggtccggcc acgcatcgag
     4501 acatcgcagc ctttgcgagg ggccgaccca agctcgctgc tgcgcgagcc gagcgttcct
     4561 tcgccgaata cctcgtacgc acatcgacgg cgctccagat gcgcggcgct ctcttcgaag
     4621 cgctcgatcg cctggagctc gcactcgcgt cgatctccac cagaggctcg agcgaagcag
     4681 agacggcgct ggacctctat gagcgcatcg gaaggctgtc ggtcgaggcg caggtcgggg
     4741 accgaggcat ccatcaaatg acccgcgcgc tcgacctagc cgacagccta ggtcgcgacc
     4801 gacaggcagc gctcttctgt gcattgcgag gagagctgct ggcgcaggcg cagcggaagg
     4861 atgaaagtcg cgattggctc gagcgcgcag cgatgctccg ccgctgagag actacttgct
     4921 gtcgggcgtt tcggtttctt ccgcggcttc ttcagcctcg agctccgctt cgatttcagc
     4981 ttcgacttca gccccgacga ccgcctcttc gagctcctcc tcttcgacct ccgctgcgac
     5041 ctcctgcgcg gccgcttgct cgcggaccgc ccggtcgagc gcctgctcac cagatgagga
     5101 gaggaacgcg agccagaccg aaagggccat gaacgtgaaa gcaaaccccg ccgtcaggcg
     5161 ggtgagcaga ttgcccgcgc cagcgccgcc gaaaatttgc tgactcgcgc caccgccaaa
     5221 agccgatccg agaccgccgc ctttgcccga ctgcaagagc acgacgagga tgaggaaaag
     5281 acacacgaat acataaacga tcgaaatgaa agtatacatg agctcagctc tccgcccggc
     5341 gaacgccggc atcgataatt ggaatgaagt tgtcggttga caaggacgcg ccgcccacga
     5401 gcgccccatc gacgtccggc tgcgagagca gctcgtgcgc gttgctgggt ttcacgctcc
     5461 caccatacag aatccgaacg gaatccgccc aggatctacc tttgagggct gaaaggtgac
     5521 cacgaatcgc aaggtggacc tcctgggcat cttccgcctt tgcggtgcgg ccggtcccga
     5581 tcgcccagat cggctcatac gcaacgacca gcggcgcgag ggcgacggca tccagcccct
     5641 cggtcgcggc ctcgacttgg cccaggacga ccttgagcgt ctcgccagcc tcacgctgtt
     5701 cgaggctctc cccgacgcag agaataggca cgactttgtg cacgagcagc gcggagacct
     5761 tcttgcgtac acccgcgtcg gtttcgccga agaactgacg gcgctccgag tgcccgatga
     5821 tgcagtaccc acaacccgcg tcgaccagca gagcggggct gagctcgccg gtccaggcgc
     5881 cggaatcttc ccaatgggtg ttctgcgcgg aaaggccgac caccgagacc cggaccgact
     5941 cccgtacggc cgcgatcgaa gtggcgaccg gcgcgacgac cacatcgcag gccaagccag
     6001 tccccacatg cgcggcaatc gcgccggcga gggcctgcga ttcggcgatc gtgttgtgca
     6061 gcttccagtt gccagcgatg agcggtttgc gtttcgtcat ggcttcagag cctccacacc
     6121 tggaagtgac cggccctcta gatactccaa cgaggcaccg ccgccagtgg acacgtggtc
     6181 gaagcgattc gcgaggttcg cctgtttcac tgcggccacg ctgtctccgc cgcccaccac
     6241 gctgaacccg gggcaatccg ctgctgcacg ggccacggca agggtgcctt cggcaaatgc
     6301 cgcgttctcg aagaggccca tcggcccatt ccagaacacc gcgagcgctc ggcgaaggcg
     6361 ctctcggtag agcctttcgg ttgccgggcc gatgtcgagc gccatcgaat ccgcggggat
     6421 cgcatcgacg ccggtcactc gtgcttcggt ggattccgtg gattcaccga cgcgcacgtc
     6481 gatcggaagc agaaggtcga cacgcttttc ctcagccgcc gccatgaggc ttcgagcgag
     6541 ggcgagcttg tcgcgctcgc agaggctctt cccgacgtcg aatccgcgag ccgcgaggaa
     6601 cgtattggcc atggcaccgc cgatcaggag tgcatcgact ttcgtgagca gggcctccat
     6661 gactccgatc ttgtcactga ctttcgcgcc gccgaggatc gcgatgtatg gacgaggtgc
     6721 atcggttcga agccggtcga gcgcctcgag ctcccgctgc atcagaaaac ctgccgcgcg
     6781 ctcttcgatg agaccgggga gggcgctcac cgaggcgtgc gctctatgcg cagcaccaaa
     6841 agcgtcgttc acgtaagctg tgcagccgct tccgagctcc cgcgcgaagt ccacatcgtt
     6901 gctcttctct tcggggtgga accgcaaatt ttcgagcagc gcaacctgtc catcgcgcag
     6961 atcttgaacg accttgcgcg caccgtctcc gatgcagtcg tcggtgagac gcacttcgcc
     7021 gtcttcgagc aactctgcga ggcgcactgc gacgggttcg agcgaaagcc ctggcacgac
     7081 ttggcccttg ggccggccga ggtgcgaggc caggacgacg cgggcgcctt gctcgagtgc
     7141 gtagcgaatc gttgggagtg ccgcacgaat ccgcgtgtcg tcgccgatct ctccgtcttt
     7201 gatggggacg ttgaaatcga cccgaatgaa ggtcgtcaca ctttcgaggc tcaggtcctc
     7261 gattgaacgg atcggctcag acacgcgctc aatccgcgct cgaaacgatc cgcaccagat
     7321 cgaccatgcg gttcgagaag ccccattcgt tgtcgtacca actctgcact tcgaccaggt
     7381 cgccgccgac cacctgggtc tgcgccgcat cacaaatcga tgagtgcggg ttacccatga
     7441 aatcttgcga aaccaggggc ctctcttcga agtaaaaaat gcctttgagc gggccctctg
     7501 cggccgcttt gagagcgcgg ttgacctctt cacggtcggt gttgcgaccg acctgtgccg
     7561 tgaggcaagt gagcgaaaca tttggggttg gcacgcgaat cgcggtgccg tcgagcttgc
     7621 ccttgagctc gggaaggacg aggccgatgg ccgttgccgc gccagtcgaa gtgggaatca
     7681 tcgagacggc ggcagcgcga gcacgacgct tgtcggagtg cggttggtcg agaaggccct
     7741 ggtcgttcgt gtacgagtgc acggtaacca tgtgcccttt tacgatgcca aagctgtcgt
     7801 tgaggacctt cacgagcggc gccaagcagt tggtagtaca cgacgcgcag ctgatgacgt
     7861 ggtccttggc cgggtcgaac ttgtcgctgt tgatcccgac gacgaaggtg ccatcgatct
     7921 cacccttgcc gggtgcgctg atgatcacgc gctccgcgcc cgccgcgaga tgtgcagcgg
     7981 ccttttccct cgttcgaaac aagcccgtgc attcgaggac gacctcggcg ccgagctctt
     8041 tccacttgag cttcgccggg tcccgttcag cggagacggg gatccgtcgg tcgccgatcc
     8101 aaattgcgtc agcctcggcc ttgaccgggt gggaaaagtg cccgtgcacc gagtcccact
     8161 ccaggagatg agcgagcatg tctgcgctag tgagatcgtt gattccgacg atctcgacgt
     8221 ccccaccgcg ctcagcgacg attcgagcta cgcagcgccc aattcgtccg aagccattga
     8281 ttccgacttt cgtagccatt cgttcgactc cctctcagcc gcgacgcggt tcgaatacgc
     8341 cgtcagcgag ggcagaacgt agcgcgagcc aagtcctggt caaggagcca aaggctcgaa
     8401 tagtttgcgc ttttgagcag agcgaaggtc gcgcgcgaac tgctactccg ctgcttcctg
     8461 tgcgtcacga agctcagtcg tcacgagctc gaggacagca ttggtgaatg caacgatgtc
     8521 agagcggctg tggccgctgt ttcgaagctc tcggtaaacc gacttcgcga taatccgaat
     8581 gccgcggttg tccaccgctt gtgccttcgg ccccaatgtt ctgctagccc ccatattggc
     8641 caaggatgat gcagtttgcg tgccatccga agaaagcagc aaaaggctgc gattcgaggc
     8701 acactttaca caggaattta caaggccgca ccggttttgc cacagctgag cccaaaatcc
     8761 tcacaccttc ccaaactgcg taattggttt cgcgtttgcg cggcaagcgc gcagcctgct
     8821 gcgcacgtaa cctgccggga acagctagat ttcggctggc cgcccggatt ttgcttcgct
     8881 cgattcgatc gcctcgcgaa gagagcgaag cgcgctcgtg tgcagccggc tggcccagga
     8941 cttcgaaatc ccgagctcgc gtgccatcga atcgatcgac cggccttcaa aatagaaccc
     9001 acggaccagg attcgctgac ggggcgataa cctcggcagc gccgcaagaa gacgggagat
     9061 gctctcgttc ttgaggagca aggcttcggg gctgtcttct gcgaagcgcc cctggagcac
     9121 cgtgccggtg gtgaggccgg cgctcatctg catgaacact cggtcgaacg cgcctggcgc
     9181 ggccgtaggg accttctcag aggacgcctg gaggcgctca tgggcccggc gcgggagctg
     9241 ggccatcttc ctcaccccat ccaacatggc gccccggacc cggtggtacg cgaaggtctg
     9301 aaattgaacg ccgcggccgg gatcgaagcg atgatgggcc tcgagcaggc cgccaaagcc
     9361 aaaggcgatc agatcctcga gctcgccgct gagcgagagc tcgcgccgga ggcggtgggc
     9421 gaggccgtgg accatttttt cgtgtgctgc gatgaactcg gatccttgca tgccaactcc
     9481 tgaacggact tcctttagcc gaaagggtca atgtggtcaa atgaaggttg tttgactaca
     9541 tacgatgaac ctggtggaat cgtcggccag ggggtatagc ggcgctgtga cggagtcccg
     9601 cccaggcatc gcaagcgcga atcgcccgcg tttgggtccg ttcgccagcc tgctcaatga
     9661 ggttccgcat gaccagagcg ccttggacgc cctcgcgttc gcgtatgctg agctggcgga
     9721 agcagagcgc ggagcgcttg tccaggcggt cgtgcaagac agcaacgacc cggcgcaggc
     9781 actgctcgcg ctcatcgccg tcgaggaaga gccgacacta cagcgacgga ttgcgggact
     9841 catccaaagg cacagccgaa tcgagaagtc tgccatcctc gagggcaccg aaaccgaggg
     9901 tgagtcgagg ctggttcagt cccggcaggg acttggatct gaatcgctcc gcatagcctg
     9961 gaaactatcg aaaatcagcg tcatcgaggt tgaacctcga acgctttcga agattgatgg
    10021 tcacttggtg cccgtcgcca ccgcagtgga caggctgctc ccgctgctct ggcggcacat
    10081 ccgggcgggc ggcaggcttc ccgagggcgc agagcgcttc gcgggattct tctcagcggt
    10141 ctagctcagc gttcgggcgc cgagtgatgg ccgccgccgt tactcagcag ggcgccttcg
    10201 aactgagtgg tctccaagag cgagccatcc ggggcatctc ttcggaccat gaagtagtgc
    10261 cgcgagacgg agtcgttgtc ctcgcgggcc acgaccatga tggaattcat accgggatga
    10321 agcggaacct cggcatcgaa gtcgagctcc ttcgggttgg cgctgccctg gttcgactcg
    10381 tagtagacct tgtgccggct cgtcgagatg tagaggtcgc gcacccgctg tccgtcggtg
    10441 gccttccctt gaatccggac cgcatcctcg ctggtgacga aggtaccgag cggcgccaag
    10501 tcgatttcag gcgaggcgtt cacggtcacc cactcgagcc caggctcctg cgcgacttgg
    10561 ccttgagccg gcgcgatcgc agcagcggcg acccagcccg gtcgcccccc gcccaagttg
    10621 acgcgctcaa acgatccgct gctggccgta tgcagcaagc gggtcggctt ttgcacgatg
    10681 gccactaggg gagcattgct atcaggccac tcacggacct cggttccagc ctcgaggctc
    10741 accacgcgct tggcgggaag ggcccgcacc gcaaggtcct cacgatagat tggtaccttg
    10801 atcttctgcc ccgtgcccgt gccgatctcg gtgtcgatga cgctcaaagc cagggctgct
    10861 tctgtctgtt tgaaatcggg aagaacctgg aaagtgaagg gcacgaccca ggattcgccc
    10921 gccggaatcg aatctctacg gaaccgccca tcgtgaagca aaacaccggc tccgctcttg
    10981 ttctgtacgt tggcctgcac ctcgaaactg tcgacgtcgc tcacgttctt cactgtgaag
    11041 tagagggtga cctcctcgcc cggctgcacg cggccatcgc cgttcccgtt gaggttgtct
    11101 gcgatttgta ggccgtacgc gaaacgcggc tcggggaggg cgcggatcac cgtccggacc
    11161 tcagcgggct cgggcacgtg accgtgcgcc tcttcgaatt cgacgacgat cgcatcggcg
    11221 cggtccatca tgctgcgcgg cactcgacac acccgtttct cgtcctcggt cgtgcagttc
    11281 cccaacgtcg tggtccagga acgcgtctcg ccgggcgcga gcttgccaaa cacgagctct
    11341 cgccgatcga accaaggatt gtcgctgcgc gtggccgcgg cgagctgata caaagtctgg
    11401 ccaccttcgt tggtgacctc gacgcgtaga gaaagtggat cgcccgcgcg tgtgggctga
    11461 tcaccagagc tagtcgttgc cttgacccgc accttgctcg gtccgttgtc ggcgccaacg
    11521 ctccagtcga taccgagctt cttgagatcg cgtgcaacct tctcggtttc ctgttgctga
    11581 acacgctcga tgatcggttt cgcgctacgc agtagctgcg gtcgactcgg tgacttggcg
    11641 ttaagcacga ggctgcgcga aaaccgaatc aggaaagctt cctggtcttc gttttcctct
    11701 tcgccgggct cggcctccat caaacgcttg cgtaggtcgg cgttgaggta gtagcccatg
    11761 accactgcgg gcttttgcga atcggttgcg cttgcgtgcg tgaggtgcgc ttcgagatcg
    11821 acctcccgac ggaaaagcgc gtcgactttg aggtccatga cctcttggtc gacggtcatc
    11881 gggttgatcg cgatgtcggg aacgattccg acgccctgga tcgagacgtc gccgggagtg
    11941 aggtactgcg cgacggtgag cttgagcgca gagctgtctt ggagcggata gagaacttga
    12001 accgagccct ttccgaagct cgtctcgccg atgatcagtg cccggtcgtg gttcttgagc
    12061 gctcctgcga cgatttcgct cgccgacgcg ctctgactgt tgatcagcaa cacgattgga
    12121 tagtcgggct cagtccccgc tcgttcggcg tactcctcgg tgcgctcccg cggatcttgg
    12181 gaggacgtgg tgacgatcgg tccttcctcg agaaacgtgt cggcaacctg cacggcctga
    12241 tcgagaagac cgcccggatt gtcccgcaga tcgaggacga gcccgaccat gcgctttttg
    12301 tgcaaggacc ctagcgcctg acggagatcg tccgtcgtgt tcccctggaa gttcttgatt
    12361 cgaatgtagc cgacgccatc tcgaagcatc ctgtgctcga tcgagggcac ggggatgttc
    12421 tctcgcgtga gcggaacgcg tcggggagca gcccagtcgc ccgccttgct ctggcgccga
    12481 acgaagatgg tgactttgga gccaggcttg ccgcgcaagc gattgacggc ctcctgaagc
    12541 ggcatgttga gcgtggactc gtcctcgatt ttgacgatcc ggtcgtttcg ctgcaatcca
    12601 gccctcgacg caggcgtgtt ttcgatgggc ctgatcaccg tgagatgccc atctcgaatc
    12661 gaaatgacga ttcccaagcc accgaactcg ccgcgggtgc tggtctgcat ctctttgaag
    12721 tgttcgggag gtagcaggac ggtgtgcggg tcgagcgtgc ggagcattcc gttgacggcc
    12781 gtgtactcca cctcgttgag ctcgatgtct tcttcgttct tcagctgctc ctggagaaag
    12841 gagaacacat ccctgaatcg tcccgaaagc gcccaagggc tcgtcacgtc tttgaccgca
    12901 aactcgctct ccgcgctgtt gacctggatt ctgagagtgt ccgcgccgtc ttcgtagtac
    12961 accaggaccg gagcgacgtc cctttgaatg gcatcgaggc cggccagcat catcttttgg
    13021 tgatctacac gcttcggatc gacgtagtcc tcgttgacct ggtgaatcac acggttcatc
    13081 acctggagtt tcttcaggtc ccagggcgtc ttctcccgtg ccgcctgcgc cctcggtgag
    13141 gtgtcgatgt cgatttggag gccgttctcg acgggccagc cgaaagagac ggtgaaggcc
    13201 gccaaaacga ccaccaaacc aagtaagggc ttccagattc ttgtcattcg attttcgggg
    13261 gggcggaggg cagactttcg attgtagcct cgttggactc ccaggccaat tgcgttggtt
    13321 tgtacctaag gttatcgagg caaggtgcca catcgaatac tccgccagca gatcgccatg
    13381 ccggtgctcg aaatttgtaa ggagctccgc aatgcggggg agcgcgcctg ggtcgtagga
    13441 ggctgtgtcc gggacacctt acgtgggcaa aacgtcaacg actgggacgt ggcgaccacc
    13501 gcactgcccg aaaaggttca gaaaaccttc cgcagggtga ttcctaccgg ggtcgatcac
    13561 gggacggtga cggtgctctg gaaaggcggg gcctacgaga tcacgacact gcgcggagag
    13621 ggcgcctaca gtgacggtcg acgacccgac aacgtggttt tcgtcgagga catcgaccgg
    13681 gacctcgcgc gtcgggactt caccgtcaac gccatcgcct acgatcccgt cgacggccgt
    13741 ctagccgatc cgttcaatgg cttggacgac atgcggggca aggtcttgcg cgcggtcgga
    13801 aatcccaacg agcgcttccg cgaagacggc cttcgcattc tccggggtgc acgcttcgtg
    13861 gccaccctcg acttcgagct cgaagaagac accgaggccg cgttccgtgg tgcactcgac
    13921 acgtttcgaa aggtgagtcc cgaacgagtc cgcgaggagt ggttgaagac gatgaaagcc
    13981 cccgtgcctt ctcgcgcgtt cgaggtcatg cggcgcacag gaatcttgga ggtcacgtac
    14041 ccagagctcc tcgagcaagt gggctgcgaa cagaaccagt ggcatgcgta cgatgtctgg
    14101 gggcatacca tgcgtgtcct cgacgaatcg gcggcagacc cggtcgaacg aatcgccgca
    14161 ctgcttcacg acgtggcgaa accccgtacg cgcgccaagt ccgagaagac cggcgactgg
    14221 accttctatc accacgagaa agtcggcgct gacatggcgg agcgctggct acgcgactac
    14281 cgattctcga atcaggagcg cgagctcgtc gtgagtctca tccgacatca cctgatctgt
    14341 tacagcagcg agtggaccga tgctgcggtg cggcgattca tcaagcgggt cggggccgac
    14401 agggtcgagc cacttcttcg cctcgggaaa gcggatgcac tcggaaaagg acgtcacgtg
    14461 gacgaagagc tggcggcact gaaagagctc cgcactcgga tcgaacaatc gatcgaagag
    14521 ggcgatgcgc tgagcaccca ggaccttgcg gtgggcggtc aagacgtcat cgaacacctg
    14581 gaagggggcg ccgggccgcg cgttggcgaa atccttcgag cgctactcga ccgtgtcatc
    14641 gaggacccgt cgctgaacac gcgcgacaaa ttgatgcccc tcgtcgagaa gcttgccgcg
    14701 aagcctagcg aataggaacc gcatcggctc ggatcgcccc gacccatgcg cttttctgga
    14761 cggcgccgag cgcttcggta gcgtagggaa gcgatgaagc tctgggccgc gacagtattg
    14821 acgattgtgc tcttcctgcc ggctactggc caggcgaaga agaccgagga cttccgccat
    14881 agctacgatc aggtctgggg cgccgcagtt cgattgattc gcgtcgacca gggctatccg
    14941 atcaaggacc gggatgagac ggtcggctac ttcctattcg actatcggga cgacggccgc
    15001 ctgtacccgg gcagtgtcga gctcgtccga atccaagacc agggcggcgg tccgattcga
    15061 gtggtggtcc agatcccggc gatgccgagc tacatcgagc gcatgttgct cgacaagctc
    15121 aagaggaagt tgctcgacga atacggcgag cctgcgcctc cgccgccacc tccggatcct
    15181 actccggagg agcccgccga gccgccgtcg tccgaaccgg acaactaggc gattcgggca
    15241 aggctcgacc ttcgactgag tgccggagct cgacctcggc tactgattgg aagcaggctg
    15301 ctcgaagcaa tagatcgctt ggtcgttgaa ggcgcagtcc ttcgtttggc gcgccgtcca
    15361 gccgttttgg gagctgatcc agtagccgct ctgcgcgggt gcccagtcga atccgt
//
LOCUS       DASRZF010000433         6078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_678402, whole genome shotgun sequence.
ACCESSION   DASRZF010000433 DASRZF010000000
VERSION     DASRZF010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678402"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VFG22_19060"
     CDS             complement(<1..572)
                     /locus_tag="VFG22_19060"
                     /inference="COORDINATES: protein motif:HMM:NF019045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Na+/H+ antiporter"
                     /protein_id="HET6418405.1"
                     /translation="MTTLWMLIAWPAIAAASGQAEPPTIVFPPSLDGYGDAGFTSLGA
                     ILRNRVEKVPFNLWATMLFIGAILHTFGTHRFRHISHVLEQRHDAKIRAGETRNRVSA
                     LARVFHFLGEPEVVFALWTVPLFFTIDLFYDQATAASYINDGVNFTEAVFVVVIMTIA
                     STRPILELAEGAMRQLARLGGETPVAWWLV"
     gene            655..2250
                     /locus_tag="VFG22_19065"
     CDS             655..2250
                     /locus_tag="VFG22_19065"
                     /inference="COORDINATES: protein motif:HMM:NF044849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6418406.1"
                     /translation="MPTDVTFELRLTLDESQSPEAMRKLAAKKLGVPLEDLPELHVLR
                     RAIDARGKGVHIQVLFGLGPQAPAEEWALPHPREVSLPSRVVIVGSGPAGLFCAYELA
                     RHGIASRIIDRGKRVQPRRRDIALLNREGKVDPDSNYCFGEGGAGTYSDGKLYTRAHK
                     RGSVRDVLEILALHGAPAAILEDARPHIGSNKLPKVVTALRERLESVGVEIHFEAKLE
                     RLLVEGQGGNRRVSGVELADGRTFEADAVVLATGHSARDVYEMLDAMDVTLEPKAFAM
                     GVRVEHPQALINKIQYGRDAGHAALPSAYYRLATSVEDRGVFTFCMCPGGWIVPATTE
                     AGAQVVNGMSLSKRDSPFANSGIVAGIEPADWQAQGFDGPLGGIRFQRELERAAYEAG
                     GGNLRAPATMMKDFLAGRGSSTVPKSSYKPGLTATRLDEVLDFSGVRISEMLRGGLTQ
                     FARRMPGFLSHAGVLLGVESRTSSPVRIPRRDDTLESPDVRGLYPSGEGAGYAGGIVS
                     AALDGKRVAEALQQQLAPRLPLT"
     gene            complement(2243..4600)
                     /locus_tag="VFG22_19070"
     CDS             complement(2243..4600)
                     /locus_tag="VFG22_19070"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HET6418407.1"
                     /translation="MRPAQLFGVAIALLTTQALFATAGMAQEAQFDRTELPIKGPWYP
                     PITTLDARDAKAPPVFQVTAPKDAPNVVVILLDDLGFGGTSAFGGVIETPNIDHLASQ
                     GLMYNQFHTTALCSPTRQALKTGRNHHSANQAKITEVATSFPGATGVLPNDVAGIGEM
                     LRLNGYSTAAFGKWHETAVWEISPSGPFARWPNLQGFDEFYGFLGGETNQWAPAVYHN
                     FNRIETPDDPSYHFMNDMATRAIDWIRFQQALTPERPFFVYFAPGAVHAPHHVPKSYI
                     EKQKGRFDEGWDVIRKRIFEQQKKLGIIPRGTKLAKKPDYIKDWKRLSAKEKKLFARQ
                     AEVFAAYLDMTDHETGRVIQAIEDMGEVDNTLIIYVIGDNGTSAEGLANGLYNEMTYF
                     NSEPRGSDVDFMLEHYDDWGGPTTYPHMAAGWAVAFDAPFTWTKQVASNYGGTRNGMI
                     MRWPDRIKAKGEVRSQWHHVIDVVPTILEAAGLPEPRIVNGTPQRPIEGVSMVYSWDD
                     ARSPSRHQIQYFEMFGNRGVYFDGWFAGTVHVAPWGKVENRFTEDVWELYHVEEDFSM
                     SKNLAKKHPEILTELQKVFLAEAIKYKVLPLDDRREELFNPKLAGRPDLMFGRKSLTL
                     YAGMNGLLENDFINIKNTSFEIVAEIETGEKPVNGVIVAQGGRFGGWSLYVKDGKPVF
                     MYNYLGIERYTATGSATLPKGKSTVKMDFVYDGGKPGAGGTATVFVNGKSVGSAKVAK
                     TQFAVFSADETAGVGIDTETPVSEDYTRANSAFTGTIDKVTIQLK"
     gene            4753..5175
                     /locus_tag="VFG22_19075"
     CDS             4753..5175
                     /locus_tag="VFG22_19075"
                     /inference="COORDINATES: protein motif:HMM:NF017936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET6418408.1"
                     /translation="MNPTEIIAALGLVPHPEGGHYRETWRHENGEERGSGTAIYYLLQ
                     AEERSHWHRVDAAEAWHFYAGSPLELLTHVEGAEVVFQVLGPDIVAGERPQIIVPPNA
                     WQAACSLGDWTLVGCTVSPAFEFGGFELARKGWSPTSR"
     gene            complement(5198..>6078)
                     /locus_tag="VFG22_19080"
     CDS             complement(5198..>6078)
                     /locus_tag="VFG22_19080"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET6418409.1"
                     /translation="AMNSTVTHLEDYDTEQQFEAVVVSSERITAEASGIEVREITLDV
                     NKPDFEFILGQSIGVLAPGSKAFGQKHHFRLYSVADLPERGGSGLPRIKIVVRRASYV
                     DKYSGEEYPGVASNYLCDLAPGDRITMSGPYGLAFKLPEEMDANLILIGTGTGIAPFR
                     AFIKHLYKNVPDWKGTVTLFYGAQSGLELLYMNDERDDFAQYYDRETFQAFKALSPRP
                     SWAAPIDWDGALAERGAELWRLLEQPNTYVYVAGLEKMRGELDKAFKEIAGSAQKWAR
                     RKAELSAGGRWVELLY"
BASE COUNT         1085 a         1836 c         1953 g         1204 t
ORIGIN      
        1 aagaccaacc accatgcgac cggggtttcg ccgcccagcc tcgcaagctg gcgcatggca
       61 ccctcggcca attccagaat tggtctcgtc gacgcgatgg tcatgatcac cacgacgaac
      121 acagcctccg tgaagttcac accatcgttg atgtaggaag cggcggtggc ttgatcgtag
      181 aagagatcga ttgtgaaaaa tagcggaacc gtccagagcg caaagaccac ttcgggttcg
      241 ccgaggaagt ggaatacgcg tgcaagcgcc gacacacggt ttcgcgtttc gccggcgcga
      301 attttcgcgt cgtgacgctg ctcgaggacg tgcgaaatgt gccgaaaccg gtgcgttcca
      361 aaggtatgga ggatggcgcc gatgaacagc atagtggccc agaggttgaa gggcactttc
      421 tcgacgcggt ttcgaaggat ggccccgagg ctggtgaagc cggcatctcc atagccgtcg
      481 aggctcgggg ggaaaacgat cgttgggggc tcagcctggc ccgaggcagc cgcgattgcc
      541 ggccaagcga tgagcatcca aagagtcgtc acggctgcca acttgcataa gcgaaggctg
      601 aaacgtttca cagcacccat ctttcgcgcg tatgctatca gctgtcagaa caccgtgccc
      661 accgacgtca ctttcgagct tcgactcaca ctcgatgaat cccagagtcc cgaggcgatg
      721 cgtaagctcg ctgccaagaa gctcggggtg ccgctcgaag acttgccgga gcttcacgtc
      781 ttgcgccgcg ccatcgatgc acgcggaaag ggcgtccata ttcaagtcct gttcggactg
      841 ggtccgcagg cccctgccga agaatgggcg cttccgcatc cccgagaagt tagtctcccc
      901 tcacgggtgg tgatcgtagg gtcggggccg gcaggtctct tctgcgcgta cgagctggcg
      961 cggcacggca tcgccagcag gatcatcgac cgcggcaagc gagtccagcc ccggcgccgt
     1021 gacatcgcgc tcctcaatcg tgagggcaaa gtagatcccg acagcaacta ctgcttcggt
     1081 gaaggcgggg cggggaccta cagcgacggc aagctctaca cgagggcgca caagagaggc
     1141 tcggttcgtg acgtgctgga gatcctcgcc ttgcacgggg ctccggcagc cattctagag
     1201 gatgcccggc cgcacatcgg aagcaacaag ttgcccaagg tcgtaaccgc gctgcgcgag
     1261 cggctcgagt ccgtgggggt ggagatccac ttcgaggcga agctcgaacg gctccttgtc
     1321 gaagggcagg ggggcaatcg cagggtgagc ggggtcgagc ttgccgatgg gcgtactttc
     1381 gaggcggacg cggtcgttct ggcaacgggg cattcggcac gcgatgtgta cgagatgctg
     1441 gacgccatgg acgtgactct agagcccaag gcgtttgcca tgggtgttcg cgtcgagcat
     1501 ccgcaggcgc tgatcaacaa gatccaatac gggcgcgacg cgggtcatgc cgcgctgccg
     1561 agcgcgtact accgattggc gacgagcgtc gaggaccgcg gcgtattcac gttctgcatg
     1621 tgcccgggcg gttggatcgt tccagccacg acagaggccg gcgctcaggt cgtcaatggc
     1681 atgagcctct cgaagcgaga ttcccccttt gcaaactcgg gcatcgtagc ggggatcgag
     1741 ccggcagatt ggcaagctca agggttcgat ggaccacttg gaggcatccg ctttcagcgc
     1801 gagctcgaac gagccgccta cgaggccggt ggaggaaatc tccgagcgcc ggccacgatg
     1861 atgaaggatt tcctagccgg ccgcggctcg agcacggttc cgaagagcag ctacaagccg
     1921 gggctgacgg ccacgcgtct cgacgaggtg ctggacttca gtggggttcg catctcggag
     1981 atgctgcgcg gcggactcac tcaatttgcc cgccgtatgc cggggttcct cagccacgca
     2041 ggcgtcctct tgggtgtcga gtcgcgaacg agctcgccag tccgtatccc gcgtagggac
     2101 gacacgctcg aatcacccga cgtgcgaggc ctttatccct ctggcgaagg cgccggctac
     2161 gcaggtggaa tcgtgagcgc cgcgctcgac ggaaagcgcg tcgcggaggc gctgcaacaa
     2221 cagctcgcgc cgcgtcttcc gctcacttga gttggatggt caccttgtcg atggtgcccg
     2281 tgaacgcgct gttcgcgcgc gtgtagtctt ctgacaccgg cgtttccgtg tcgatcccga
     2341 cgcccgccgt ttcgtctgca gagaagaccg cgaactgggt ctttgcgacc ttggcggagc
     2401 cgacggactt gccattgacg aagacggtgg ctgtgccacc cgctcccggt ttgccgccat
     2461 cgtaaacgaa atccatcttc acggtcgatt tccccttggg cagcgtcgcg cttccggtcg
     2521 cggtgtaacg ctcgattccg aggtagttgt acatgaagac cggcttgccg tccttgacgt
     2581 agaggctcca gccaccgaag cgcccgccct gcgcgacgat cacaccgttc accggtttct
     2641 cgcccgtctc gatctcggcc acgatctcga aagatgtgtt cttgatgttg atgaaatcgt
     2701 tttctaggag gccgttcatg cccgcgtaga gggttaggct cttccgccca aacatcaggt
     2761 cgggtcgccc ggccaacttg ggattgaaga gctcctcccg ccggtcgtcg agaggcaata
     2821 ccttgtactt gatggcctcc gctaggaaca ccttctggag ctcggtcagg atctccgggt
     2881 gcttcttcgc gaggttcttc gacatgctga agtcctcctc cacgtgataa agctcccaga
     2941 cgtcctccgt gaaacggttc tcgactttgc cccagggcgc gacgtgcacc gtacctgcga
     3001 accagccatc gaagtagacg ccgcgattgc cgaacatctc gaagtattgg atttggtgcc
     3061 gactgggcga tcgggcgtcg tcccagctat agaccatgct cacaccctcg atcggccgct
     3121 gcggcgtgcc gttgacgatg cgcggctcgg gcagtccggc ggcttcgagg atggtcggga
     3181 ccacgtcgat gacgtggtgc cattgcgagc ggacctcgcc tttcgctttg atccgatccg
     3241 gccagcgcat gatcatgccg ttgcgcgtac cgccgtagtt cgaggcgact tgtttggtcc
     3301 aggtgaaggg cgcgtcgaaa gcgaccgccc atccggcagc catgtgcgga taggtcgtcg
     3361 ggccgcccca gtcgtcgtag tgctccagca tgaagtcgac atccgagccg cggggctcgc
     3421 tattgaagta cgtcatctcg ttgtacaaac cgttcgccag cccttcggcg ctcgtgccat
     3481 tgtcgccgat gacgtagatg atgagcgtgt tgtcgacctc acccatatcc tcgatcgcct
     3541 ggatcacccg gcctgtctcg tggtcggtca tgtcgaggta agccgcaaac acctccgcct
     3601 ggcgcgcaaa gagtttcttt tccttggctg agaggcgctt ccagtccttg atgtagtcgg
     3661 gcttcttcgc cagcttcgtg ccgcgtggga tgatgccaag cttcttctgc tgctcgaaga
     3721 tgcgctttcg gatcacgtcc cagccttcgt cgaaacggcc cttctgcttc tcgatgtagg
     3781 actttggtac gtggtgcggg gcgtgcaccg cgcccggcgc gaagtagacg aagaatggcc
     3841 tttcgggtgt gagcgcctgc tggaaacgga tccagtcgat ggcccgcgtc gccatgtcgt
     3901 tcatgaagtg ataactggga tcgtccgggg tctcgatacg attgaagttg tggtagacgg
     3961 ccggcgccca ctggttggtc tcgccgccga ggaagccata gaattcgtcg aagccctgca
     4021 ggttcggcca gcgggcaaag ggccccgagg ggctgatctc ccagaccgcc gtctcgtgcc
     4081 acttgccaaa cgcggcagta ctgtagccgt tgagccgcag catctctccg atgccggcga
     4141 cgtcgttcgg cagcacgccc gtcgcgcccg ggaaggacgt cgccacttcg gtaatcttcg
     4201 cttgattggc cgagtgatgg ttgcggcccg tcttgagggc ctgtcgtgtc ggtgaacaga
     4261 gtgccgtcgt gtggaactga ttgtacatca ggccctggct ggcgaggtga tcgatgttcg
     4321 gcgtttcgat gacgccgccg aaggcgctag tcccgccaaa gccgagatcg tcgagcagga
     4381 tgacgaccac gttcggcgcg tctttcggcg ccgtcacttg gaagacgggc ggcgccttgg
     4441 catcgcgcgc atcgagggtc gtgatgggcg ggtaccaggg tcccttgatc ggaagctccg
     4501 tacggtcgaa ctgagcctct tgcgccatgc ctgccgtcgc gaagagggcc tgcgtcgtaa
     4561 gtagagcgat cgctacgcca aagagctgtg ccggtctcat tcttcctccc gcccaaagaa
     4621 ggcgcttcct gcagacgaat cgcatacacc agttcggcgt cgaaagcatc ccgagcgacg
     4681 cggacagctc gagcgcgatg gcttggaatg agaccgcggt gtacgatctc ctcgtgcgaa
     4741 accgggagct cgatgaaccc aactgaaatc attgccgctt tggggctcgt tccccatccg
     4801 gagggaggcc attacaggga gacctggcgg cacgaaaacg gcgaggaacg cggatcggga
     4861 acggcgattt actatcttct ccaggctgaa gagcgatcgc actggcatcg agtcgacgca
     4921 gccgaggcct ggcacttcta cgcgggttcg cctctggagc ttctcacgca cgtcgaggga
     4981 gccgaagtgg tgtttcaggt gcttggtcca gacatcgtgg ccggggagcg accgcagatc
     5041 atcgtaccgc cgaacgcgtg gcaggcggcc tgctcactgg gcgactggac gctcgttggg
     5101 tgcacggtgt cgccggcgtt cgagttcggg ggtttcgagt tggcccgcaa aggctggagc
     5161 cccacgtcgc ggtgacgccc gttcgaaacg atagccctca gtagaggagc tccacccagc
     5221 ggccgccggc gctgagctcg gccttgcggc gggcccactt ctgcgcggag ccggcgatct
     5281 ctttgaaagc tttgtcgagc tcgccgcgca tcttctcgag gccggcgaca tagacgtacg
     5341 tgttgggttg ctcgaggagc ctccagagct ctgcgccgcg ctcggcgagg gcgccgtccc
     5401 aatcgatcgg agcagcccag gagggtcggg gactgagcgc cttgaacgcc tggaaggtct
     5461 cgcggtcgta gtactgggcg aaatcgtccc tctcgtcgtt catgtagaga agctcgagcc
     5521 cgctttgggc gccatagaag agcgtcaccg tccctttcca atctgggacg ttcttgtaca
     5581 ggtgtttgat gaatgccctg aacggggcga tcccggtgcc cgtcccgatg aggatcaggt
     5641 ttgcgtccat ctcttcgggc aacttgaacg cgaggccgta cggacccgac atcgtgatgc
     5701 ggtcgccagg cgccagatcg cagaggtagt tgctcgcgac gcccgggtat tcctcgccgc
     5761 tgtacttgtc cacatagctc gctcggcgta cgacgatctt gatccgcgga aggccgctgc
     5821 ctccgcgttc gggaaggtcg gccacgctgt agaggcggaa gtggtgcttc tgtccaaacg
     5881 ccttgctgcc gggcgcgagc acaccgatgc tttggccgag tatgaattcg aagtcgggtt
     5941 tattcacgtc tagcgtgatc tctcggacct cgatgccgga ggcctccgcc gtgattcgct
     6001 cgcttgacac cacgactgcc tcgaactgct gttcggtgtc gtagtcttca agatgggtga
     6061 ccgtactatt catcgcat
//
LOCUS       DASRZF010000434         6326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3435135, whole genome shotgun sequence.
ACCESSION   DASRZF010000434 DASRZF010000000
VERSION     DASRZF010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 6326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6326
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3435135"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            332..880
                     /locus_tag="VFG22_19085"
     CDS             332..880
                     /locus_tag="VFG22_19085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HET6418410.1"
                     /translation="MHPWHDVYIDEDQLETVFPVVIEVPMGSKNKYELDKESGLLRLD
                     RVLFSAVHYPANYGFVPRSFCDDGDPLDALVLGQEAVVPLTIMNARAIGVMRMRDDKG
                     IDDKIIAVSVNDPVYQGLKELEDIPEHKLREIQRFFEDYKALENKEVVVEGMQERDEA
                     IQILRNGLVMYRQLRRGEIPKG"
     gene            1092..1826
                     /locus_tag="VFG22_19090"
     CDS             1092..1826
                     /locus_tag="VFG22_19090"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET6418411.1"
                     /translation="MHKASWVLLLAVSLMLTAGCKKDELRAKDARIAELMEQGQATGT
                     SLEQCKKDRENLNKLVKGYTGEINAMEAALAEAAAREERAANRLAEYNSMLKQLKSMI
                     DSGKLNVRIVRNKMVIELPEAVLFASGSAKLKTDGVRVLAELGPVLASIKGREFQVGG
                     HTDNKPIRTARFPSNWELSGARAIDVGELLIEYGVPGNRISAAAYADTQPVESNDTAE
                     GRAKNRRIEIALQPNLDELPDLSSFE"
     gene            2096..2974
                     /locus_tag="VFG22_19095"
     CDS             2096..2974
                     /locus_tag="VFG22_19095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418412.1"
                     /translation="MRGFDLILRLILGGMLAVAGCSSSDGGGQGGEGGMGGSTGSAVS
                     VGGIAWAFELPGLPYGRIKGATVSILEMPELSTESNDEGEFTIEGIPVGSQATFVLQH
                     DDHPLTYTKTFTIPDSDIDDLTFQIPTNFLFGQIEDGLVEGGIIEGIDVEKCQMVSTF
                     TRYGKTIGDPGPHGEAGAVLSVAPANNAEYGPMYFNDEVLPDPTRSYSSLDGGVLLLN
                     VDPGDYTLSASCVENPSDLYDDFLAEYPPATYPDEALRCQTEDVEFEPVLMKCRPGVF
                     LNASPSYGLQAFPHAP"
     gene            complement(3149..4171)
                     /gene="dctP"
                     /locus_tag="VFG22_19100"
     CDS             complement(3149..4171)
                     /gene="dctP"
                     /locus_tag="VFG22_19100"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HET6418413.1"
                     /translation="MYELARKSWLVFVLASACLLVEGSRAPHAGAEGKTVIRYATIAP
                     GGSSFGKVLKAWGRTLSEETEGRVELRYYSGGSQGDERDFVRKMRAGQMDAAGVTTIG
                     LGIMVRQALVLSAPGVIEDYEQLAGVREKMDGRFEAMFDEAGFVLLAWADAGRGRIMS
                     TMPIRRPDDLKSARPWAWKDDPIFSEFLKVVGANAVRLGVPEVYGALQTKMVDVVPAS
                     PIAAVALQWYTKLKYMSKESFGIILGASVVKKEKFEQLSPEDQAVLRDTAKRAGAALD
                     KVVRRDDAKAYDVLLKRGIELVDTGPYRAEWDKAAAETRERLTGRIFSKSLLAEVEAA
                     AKGTGP"
     gene            complement(4175..4960)
                     /locus_tag="VFG22_19105"
     CDS             complement(4175..4960)
                     /locus_tag="VFG22_19105"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HET6418414.1"
                     /translation="MQRHVPLSGQPNFRDLGGYEASDGRTVKWGLVFRSGELSQLSEA
                     DVEKLGALGIRGVVDLRSPQEVSARGGGRLPRGATLFPMPIASSDMFAKLIPMFLKGD
                     FSEVPTDLLDRVNRMLVRDFTEPYSGLLRTLSDPGNRPLVFHCTQGKDRAGFGAAMVL
                     SALGVPWETVVEDYLLSNHFRKEENEKMLGMIRAFAASRGGPEGEEIALSRVEGLMYV
                     KEQSLQAAHREIVERHGSVAGFLTEGLGFSAEALARLRDELLE"
     gene            complement(5010..6224)
                     /locus_tag="VFG22_19110"
     CDS             complement(5010..6224)
                     /locus_tag="VFG22_19110"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET6418415.1"
                     /translation="MADVRFPSNLDELTPEVLTVLLSEQRPGARVANLRVLRSASRGD
                     GVASTADRVALELDYESDVGLPSRMLLKTVLLHRSLRFGASSIQWTGRLFRLLGALPF
                     GSRVRPAVFSLMGAYQRRFPQAPDAMYLNEVRFYREIRPLLSIEAPQCFGSLFDVKRR
                     SFGVLMEDLTLRGARFPNALDGVGIEEIRGLIETLAALHARFWESAELQSRLRWLATP
                     VSGGMYPVFQALGLDLIRNQVETNPYKAALIEPLGKTVDELWSALWKAQAILSTGPQT
                     LLHGDPHIANTYLLPEGPGGFLDWQLVIKGRWAHDLTYLMITGLSTDERRAHERELIA
                     FYLDALRREGVETPPAPDEAWLRYRQAAIWGLVIGWLITPTENYGEAITAANLARLVA
                     AVEDLETLKALG"
BASE COUNT         1252 a         2141 c         1802 g         1131 t
ORIGIN      
        1 tattgatgga gcgatccgga cgaagcagaa atcgccggcc aatacatcgt cgcgtcacga
       61 gataggtggt gtttgggagc acgcaccggg gcaaggtcat gccgagtttt tcggtcagta
      121 ccccgaactg ttgtccagga acgaacctcg gaaagttcga gttgaattct agggctagtc
      181 ccgctgcggg tgcgtcacta acctgggaag atcgggtttg gggtggcgtc ggtcgcgggt
      241 tttcggattc gcgtggggcg cccaggtgct atgctccggc ccactccagc tatccggaaa
      301 cccgactgcc ctccgccgca agaagaacgc gatgcacccc tggcacgacg tctacataga
      361 tgaagatcag ctcgagaccg tgttccccgt agtcatcgag gttccgatgg ggagcaagaa
      421 caagtacgag ctcgacaagg agagtggcct actgaggctg gaccgtgtgc tcttcagcgc
      481 ggtgcactat ccggcgaact acggcttcgt cccccgaagc ttttgcgacg acggcgaccc
      541 gctcgacgct ctcgtcctcg gccaagaagc ggtagtgccg ctcacgatca tgaacgcccg
      601 cgccatcggc gtcatgcgca tgcgggacga caagggcatc gacgacaaga tcattgccgt
      661 tagcgtaaac gacccggtct accaaggact caaggagctc gaggacattc ccgaacacaa
      721 gctccgtgaa atccagcgct tcttcgagga ctacaaagcg ctcgagaaca aagaggtggt
      781 cgtcgaaggc atgcaagagc gcgacgaagc gattcagatt ctgcgcaacg ggttggttat
      841 gtaccggcag cttcgccgcg gcgaaatccc caagggctga gcggacgccc cgcctgctcc
      901 aatcttgcag gacctaatct ccggcctacc aagataccgg ccgattccgc ctaagacgaa
      961 ccaatctagc cacctgcggt cggcgcacag tttgcgtgca tccgcgatct agcgttggaa
     1021 gagcttgcct tgccccggcc ctttgataag gtgcgcgacc ctattggaaa taatcctttt
     1081 tggaggggta tatgcacaaa gcaagctggg tactgttgtt ggctgtcagc ctgatgctga
     1141 cggcaggttg taagaaggac gagctgaggg cgaaggacgc gcgcatcgcg gagctgatgg
     1201 agcagggtca agcgaccggc acctcgctcg agcagtgcaa gaaggatcgc gagaacctca
     1261 ataagctcgt caagggttac accggcgaaa ttaacgccat ggaagctgcc ctcgctgagg
     1321 ccgctgcacg cgaggagcgc gctgccaacc gtctcgccga gtacaactcg atgctcaagc
     1381 aactgaagtc gatgatcgac tccggcaagc tcaacgttcg tatcgtccgc aataagatgg
     1441 tcatcgagct tcccgaggcg gttctcttcg cgagcggcag cgcgaagctc aagacggacg
     1501 gagttcgtgt tcttgcggag ctcggcccgg tgctcgcgag catcaagggg cgcgagttcc
     1561 aggtgggcgg tcacaccgac aacaagccga ttcgcaccgc acgcttcccc tcgaactggg
     1621 agctttcggg tgcacgcgcg atcgatgtgg gtgagcttct gatcgagtac ggcgttcctg
     1681 ggaaccgaat ctcggccgcc gcgtacgccg acacccagcc cgtcgagtcg aacgacaccg
     1741 cggaaggccg ggcaaagaac cgtcgcatcg agatcgcgtt gcagccgaac ctcgacgagc
     1801 ttcccgacct ctcgagcttc gagtaacgcc tagaatcaca catcctgtct ctcacaggac
     1861 gggcgaacca agaacgtgcc ggcgtgcatc atgcgcccgg cacgttttca attgcacccc
     1921 gcagcccgtc cttcccaaag caagcacctt cccaggttga tgagggccca gcgtcccagg
     1981 cagccgcgtc tccggaaacg ccccggtctg attcgtgcac tggcccgttt tcgttttcgt
     2041 gaccactggc gcgtttttcg aaacgttgct acgccgcagg ccttggaggc taggcatgag
     2101 aggttttgac ttgatcctga gactgattct cggcggcatg ctcgccgttg cgggttgctc
     2161 cagctcggac ggcggcggtc agggcggcga aggtggaatg ggtggcagca ccggctccgc
     2221 cgtttcggtt ggcggcatcg cctgggcgtt cgagcttccc gggttgccct acggtcgaat
     2281 caagggcgca actgtgtcga tcctcgaaat gcccgagctc agcaccgagt cgaacgacga
     2341 gggcgagttc accatcgaag gcatcccggt cggctcgcaa gcaacgttcg tgctccagca
     2401 cgatgaccac ccgctcacct atacgaagac gttcacgatt cccgattcgg acatcgatga
     2461 cctcaccttt cagattccga ccaactttct ctttggccag atcgaggacg gcctggtcga
     2521 aggagggatc atcgaaggga ttgatgtgga gaagtgtcag atggtcagca cgttcacccg
     2581 ctacggcaaa acgatcggcg accctggtcc ccatggtgaa gcgggcgcgg tcctctccgt
     2641 cgctccggcg aacaacgccg aatacggccc catgtacttc aacgacgagg tgttgccgga
     2701 tccgacgcgg agctactctt cgctcgatgg cggcgtcctc ctgctcaacg tcgaccccgg
     2761 cgactacacg ctcagcgcgt cgtgcgtcga gaacccgtcc gatctctacg acgacttcct
     2821 cgccgagtac ccgcctgcga cctaccccga cgaggccctc cgttgccaga ccgaagacgt
     2881 ggagttcgag ccggtgctga tgaagtgccg ccccggggtc ttcttgaacg cgtcgccctc
     2941 gtatggactt caggcgttcc cgcatgcgcc gtagctagtt gccattggcg gcggcctcga
     3001 ctcccgccaa gagggtcttg ctgaagatgc ccggaccaac ttcccaggtt aaggctggat
     3061 cgtcccaggt tgggggaccg ggttggatca tcccaggttg ggattcgcgg ggttcagttg
     3121 agatgccccc cggagccggg cgcgcgcgtt agggcccggt ccccttggct gcggcctcca
     3181 cctcggccag caagctctta ctgaaaatcc gacctgtcag ccgctcgcgc gtctcggcgg
     3241 cagccttgtc ccattccgca cggtaaggcc cggtgtcgac cagctcgatc ccacgcttga
     3301 gcaaaacgtc gtaagccttc gcatcatccc gccgcacgac tttgtcgagc gccgcccccg
     3361 cgcgcttcgc cgtgtcccgc agcaccgcct ggtcctcagg agaaagctgc tcgaacttct
     3421 ccttcttcac caccgacgcg ccgagaatga tcccaaagct ctccttcgac atgtacttga
     3481 gcttcgtgta ccactggagc gcaaccgcag cgatcggcga cgccggcacc acgtccacca
     3541 tcttggtctg caaggccccg taaacctccg gcacacccag ccgcaccgca ttcgcgccaa
     3601 caaccttcaa aaattccgaa aagatcgggt catccttcca cgcccagggc cgcgccgact
     3661 tcaagtcgtc gggcctccgg atcggcatcg tcgacatgat ccgtccccgc cccgcgtccg
     3721 cccaagccag caagacgaac cccgcttcgt cgaacatcgc ctcgaagcga ccgtccatct
     3781 tctcccgcac gcctgccagt tgctcgtagt cctcgatgac tcccggcgcc gacaacacca
     3841 gcgcctgccg caccatgatg ccgagcccaa tcgtggtcac cccggccgcg tccatctgcc
     3901 ccgcgcgcat cttccgaaca aaatcccgtt cgtcgccctg acttccgccc gagtaatatc
     3961 gaagctcgac tcgcccctcg gtctcctcgc tgagcgtccg cccccaagct ttgagtacct
     4021 tcccgaacga cgatcctcca ggcgcgatcg tcgcgtaccg aatgaccgtc ttcccttcgg
     4081 cgcccgcatg cggcgcacgg gagccttcga ccagcaagca cgccgaagcc aacacgaaca
     4141 ccaaccaaga cttcctcgct agttcgtaca cttcctactc caacagctca tcgcgcaagc
     4201 gagcaagcgc ctccgcagaa aatcccagcc cctccgtcag aaacccggcg acgctcccgt
     4261 gccgttccac tatctcacgg tgcgcggcct gaaggctttg ctctttcacg tacatgagcc
     4321 cttcgacgcg tgaaagcgca atttcttcac cctccggtcc gccgcgactc gccgcaaaag
     4381 cccgaatcat cccgagcatc ttctcgtttt cttccttccg aaagtgattg gacagaaggt
     4441 aatcctccac aaccgtttcc caaggcacgc caagcgccga caaaaccatc gccgcgccaa
     4501 accccgcccg gtccttgccc tgtgtgcaat gaaacacgag cggccgattc ccgggatcac
     4561 tcagtgttcg gagcaagccg ctatacggct cggtaaaatc gcgcaccaac atccggttca
     4621 cgcgatcgag caggtccgtc ggcacttcgg agaaatcccc cttcaagaac atcggaatca
     4681 gcttggcgaa catgtcactc gacgcgatcg gcatcgggaa gagcgttgcg ccccgcggca
     4741 atctaccacc ccccctcgcc gacacttctt gtggcgatcg cagatcgacg acgcctcgga
     4801 tccccagcgc gccgagcttc tccacgtccg cctcgctcag ctgactgagc tcaccggacc
     4861 gaaacacgag cccccacttc accgtgcgcc catcgctggc ttcgtaacca ccgaggtccc
     4921 ggaagttggg ctggccggac aagggaacgt gtcgttgcat agactcttct atggcctccg
     4981 acccggtcgc gcgccatccc tgcggaggct cagccaagcg ctttcaaggt ttctaggtcc
     5041 tcgacggccg cgacgagccg cgcgagattg gccgccgtga tcgcttcgcc gtagttctcc
     5101 gtcggtgtga tgagccatcc gatcacgagt ccccagatcg ctgcttgcct gtatcgcagc
     5161 caggcctcgt ccggcgcggg cggcgtctcg accccctctc gtcgaagcgc gtcaaggtag
     5221 aacgcgatga gctcccgctc gtgcgcgcgg cgctcatccg ttgagagccc ggtgatcatc
     5281 agataggtca ggtcgtgcgc ccagcgtcct ttgatgacga gctgccagtc gagaaaccca
     5341 cccgggccct cgggcagaag atacgtattg gcgatgtgcg ggtctccatg cagcaaggtc
     5401 tgcggccccg tcgacaagat cgcctgcgcc ttccaaagtg cgctccaaag ctcgtccacc
     5461 gtcttaccga gcggctcgat cagcgccgcc ttgtaaggat tggtttccac ctggttccga
     5521 atgaggtcga gcccgagggc ctggaacacc ggatacatgc cgcccgacac cggtgtggcg
     5581 agccaccgaa gccgcgactg caattccgcg ctctcccaga accgcgcgtg cagcgctgct
     5641 aaggtctcga tcaagccccg tatctcctcg atgccaacgc catcgagcgc attcggaaac
     5701 cgagccccgc ggagagtgag atcctccatc agcaccccaa agcttcgacg cttcacgtcg
     5761 aagaggctgc cgaagcactg aggggcctcg atcgaaagca gcggccgaat ctcacggtag
     5821 aaccgaacct cgttgaggta catcgcatcc ggtgcctgcg gaaaacgccg ctggtacgcg
     5881 cccatgagcg aaaacaccgc cggacgaaca cgcgacccga acggaagcgc gccgagcagc
     5941 cggaacagcc tgcccgtcca ctgaatggac gacgcgccaa atcgcaagct ccggtgaagc
     6001 agcaccgtct tgagcaacat ccgtgacggc agcccgacgt cggactcata gtcgagctcg
     6061 agcgcaacgc gatccgcagt ggacgcaacc ccatcgccgc gactcgcgct ccgcaagacg
     6121 cgcagattcg cgacgcgcgc ccccggtcgc tgctcgctga gcaagaccgt cagcacctcg
     6181 ggcgtgagct cgtcgaggtt cgatggaaac cggacgtccg ccatcatacg ctcatcaggc
     6241 gccttcgacg cgccgcttca ggtcctcgtt gaaccctgcg taagaaggcc cgagctgcgc
     6301 cttcaagaac gcccagacga tgggag
//
LOCUS       DASRZF010000435         8635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3771002, whole genome shotgun sequence.
ACCESSION   DASRZF010000435 DASRZF010000000
VERSION     DASRZF010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 8635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8635
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3771002"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..717
                     /locus_tag="VFG22_19115"
     CDS             109..717
                     /locus_tag="VFG22_19115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418416.1"
                     /translation="MSEPPTLLIPEIDEDRTLVETRNANLDCSLAELSGERGPSWPVA
                     DRNARASRCASSVGRKLRLRRCAEGIVVFGLVAGLTFVGYQQWRIAESIRKSISGFGN
                     TESPPPNHDASPPQERVPRQIEPASDPNSMSQETLEARRDRLESVGAALIRANDFEAA
                     LNHYRALAQALPGEAVFRDLVLVLQSKLRCERSLSLTNVGCP"
     gene            714..1376
                     /locus_tag="VFG22_19120"
     CDS             714..1376
                     /locus_tag="VFG22_19120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418417.1"
                     /translation="MSKWTIALASVLTLVSCADSRSVDRPFSIVFIVESDPGLPLEGV
                     NVLVDGKSIGKTDANGSVRAEILAIPGARLQIEQQCPKGHQATEDAKSIRVRKFERLE
                     EAAPRTIQVTLKCKPLQRLAVFIVTARNGPDLPVFIDGNNVGRTNQLGVAQISRWGVP
                     GTEYAVEIDTRADPRLLPRSPVHVFTLPDAHEIFVVNESFQLQERRRSGHRRRARITK
                     IE"
     gene            1406..2752
                     /locus_tag="VFG22_19125"
     CDS             1406..2752
                     /locus_tag="VFG22_19125"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin monoamine oxidase family protein"
                     /protein_id="HET6418418.1"
                     /translation="MGDRTDVVVVGAGLSGLCAARKLRAEGASVLVVEARDRVGGRTR
                     TEQIGQGTFDLGGQWIGPGHKRVHALANELGIQTFPTHTDGKKILEVEGKVSTYKRSI
                     PSMSVPNLIQMQGALSYLKRVRKRISPTAPMSAEGAEELDAETLETWRSRFVKSSKIS
                     AVMDAAIRTIFGAEARDLSALYFLMYLNAGGGLLSLSEVRGGAQQDRFVLGAQSISRA
                     LAEELGDALKLGVAVRKLVQSQDGVEAVSDDETLGGRYALVAVPPTLAGRIEYQPSVS
                     VGRDQLTQRFAMGAAVKVLVTYERAFWREAGFSGEVVSSDGPLSVVYDNTSHDRQQPA
                     LVGFVVGSQARQWSAQPLADRKRRVVGALKRYFGEEAESLQEYHELDWGAEPWSRGCP
                     VGGLPPGMLTHSFAHLRKPEGRIHWAGTEAATEWIGYMEGAIESGERAADEILRRL"
     gene            complement(2776..5076)
                     /locus_tag="VFG22_19130"
     CDS             complement(2776..5076)
                     /locus_tag="VFG22_19130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HET6418419.1"
                     /translation="MTRERPSRAEPPETEDDLRVGAPQTKAAGLASVSSAFAHMLKHG
                     HSLPSAVRILGQMNQTDGFDCPGCAWPDPDDRAALTEFCENGAKAAAWESTRRRLDWK
                     FFQQYSIDDLATRSDYWLGLQGRLTEPLMLRPGSRFYEPIDWKEAFDLIAAGLKGLDS
                     PNEAIFYTSGRTSNEAAFLYQLFVRAFGTNNLPDCSNMCHESSGVGLGETVGIGKGSV
                     TLEDIHEADTIIVMGQNPGTNHPRMLTALQKAKKNGAKIIAINPLPEAGLMGFVNPKS
                     PSQVLTGGTQIADLFLQVQINGDVALLKALMCVLLELEAKRPGEVLDEDFIGLYTEGF
                     AELRAHLESQDIDALIEAAGIERGELERAVEIIVRSRKTIICWAMGLTQHKNGVGNVR
                     EIVNFLLMRGSIGVPGAGTCPVRGHSNVQGDRTMGIHDRPPKALLDRIEEVFGFDPPR
                     EDGFNTVEAIHAMLDGRAKLFFAMGGNFLQATPDTERTAHALEACEMRVHVSTKLNRS
                     HLVHNKWTFILPCLARSEIDEQAYGPQFVTVENSMGVVHSSRGHLKPAHPQLYSEPGI
                     VAWLAQRVLGPENPVSWCWLIEDYDRIRDLIAETIPGFEDFNERVREPGGFHLHNGAR
                     ERRFNTESGKAKFTINQAPDLHLPDGSYRMMTVRTHDQYNTTVYGNDDRYRGIRGGRR
                     VILMNESDMLEAGFSAGAKVDITNEFGGRLRIAPRFTVVPYPIPKRCVATYFPEANVL
                     VPLNNYAEGSYTPASKDVTVSLTLSA"
     gene            complement(5076..5954)
                     /gene="fdhD"
                     /locus_tag="VFG22_19135"
     CDS             complement(5076..5954)
                     /gene="fdhD"
                     /locus_tag="VFG22_19135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HET6418420.1"
                     /translation="MARTPVASTKVERIERERREVCSDLVAVEEPLQIILEYGEAHRR
                     RETPLAMTMRTPGHDHDLVAGFLYAEGVVAGISDLLSVRHCARSETPHNVVRAVLREN
                     VVVPSRLLERNLTVTSACGVCGERTVESLSSEGCGRISVEGDAFSPAAVRRALSSLEE
                     SQAWFRHTGGTHGAALFDASGELLLHREDVGRHNALDKLVGAWLRQSVCSPPGAFVVV
                     SSRASFELVQKTVRARFPILVAIGAASSLAIETARRFDLTLIGFARENRFNVYSRGER
                     IAEEAGEALFAQVEVG"
     gene            complement(6022..7287)
                     /locus_tag="VFG22_19140"
     CDS             complement(6022..7287)
                     /locus_tag="VFG22_19140"
                     /inference="COORDINATES: protein motif:HMM:NF013394.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HET6418421.1"
                     /translation="MNQQKQKLEFGGVPGNFAMIFGLPIFTAYLFFAVRFNDGAVLPG
                     AGADWEGFRDAMMPSWRAAEIYGVWFVFQALLQRFAPGREVLGAELPDGGRLPYRMNG
                     LFSLLATFIAVAILHWSGLFSIRELHDEFGALISVMTIFVFAFSAFLYSYGLRHGQSR
                     HLSGKFIHDFWMGTGHNPRVPPGRDGLDLKIFCEARPGLILWVLINTSFAYVQYEEYG
                     FVSTSMILVWIFQMFYIIDYFWNEEAILTTMDIKHENFGYMLAFGDLVWVPMTYSLQA
                     HYLIDRVHSLPWWGAVLIIGVNMLGLYIFRVVNLQKHKFRKDPENARIWGKKVEYIQT
                     EQGGKLLLSGFWGWSRHFNYVGDILMALSWSLPCLFGSALPYFYPIYFAILLIHRERR
                     DHNFCSKKYGADWTRYCEKVRYRIVPGIY"
     gene            7456..8325
                     /locus_tag="VFG22_19145"
     CDS             7456..8325
                     /locus_tag="VFG22_19145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418422.1"
                     /translation="MIIVTGTKRSGTSMWMQLLSAAGFPPIGDAFPRNWETTLKDANP
                     GGFWESELRRGIFHATNPDPKTGTYLFPEQTARHAVKVFIPGLIKTDRAFIGKVVATI
                     RPWRQYVRSVNRLYAMEREAQEAARKDPEERLPPAVFMSPVIEWWVENFSLFSDIVTR
                     RYPFYMVAYDSVLDQREETLRSVFGWLGDGDVDAAIRHVEPALRTQDRANDDQVSDDE
                     QGIEPEAIAVFDALYEVVLRQAPLEQAFVDRLNAANELLKPRISETIKRVAQARLERR
                     RFIEQRRASKTQL"
     gene            8362..>8635
                     /locus_tag="VFG22_19150"
     CDS             8362..>8635
                     /locus_tag="VFG22_19150"
                     /inference="COORDINATES: protein motif:HMM:NF012300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylation factor-like protein"
                     /protein_id="HET6418423.1"
                     /translation="MGAPGVGKTSLVRRFVEQRFDDDHDSSISMAISKGELQLGEVSL
                     EMMLWDPGGDEASAQYHRSFISGASGLVFVADATKPRTLDHLLEAQT"
BASE COUNT         1704 a         2531 c         2702 g         1698 t
ORIGIN      
        1 agatgggtcg cgccgtgtct cgcatgtcgc agaggtcctc ggatacgatg aaagccacgg
       61 ctatgagatt cgttatgcgt tcgaaagcca gtctttgcga gcgatcacat gagcgagcca
      121 cccactcttc tgattccgga gatcgacgag gacagaacct tggtcgaaac tcgaaacgcc
      181 aacctcgatt gctccttagc cgagctcagc ggcgaacgcg gtccgtcctg gcccgttgct
      241 gacagaaacg cgcgtgcatc gcgttgcgct tcgtccgtcg gccgcaagct ccgacttcgc
      301 aggtgcgctg aaggcatcgt ggttttcggt ctagtcgcgg ggctgacctt cgtgggatat
      361 caacagtggc gcatcgctga atccatccgg aagtcgataa gcgggtttgg gaacaccgaa
      421 tcgcctcctc cgaaccacga cgccagccct ccacaagaac gagtcccgcg ccagatcgag
      481 cctgcctcgg accccaactc gatgagtcaa gagacgctgg aagctagaag agatcggttg
      541 gagagcgtcg gtgcggcctt gattcgtgcc aatgacttcg aagctgcgtt gaaccactat
      601 cgagcactcg cgcaggcact tcccggggaa gccgtgttcc gcgatctcgt cctagtcctc
      661 cagtcgaagt tgcgttgcga gcgttcgctt tccctcacca acgttggatg tccgtgagca
      721 agtggaccat cgctcttgca agtgtgttga cactcgtgtc ctgcgccgac agccgtagcg
      781 tggacagacc cttctcgatc gtattcatcg tcgagtcgga tcccggtctt ccactcgaag
      841 gcgtgaatgt gttggtggac ggcaagtcga tcggtaagac cgacgcaaat gggtcggtgc
      901 gagccgagat tctcgccatt ccgggagcga gacttcagat cgaacagcaa tgcccgaaag
      961 gccatcaagc caccgaggat gccaagagta tacgcgtgcg caagttcgaa aggctcgagg
     1021 aggccgctcc gaggaccatc caagtgaccc tgaagtgcaa gccgctacaa cgcttggcag
     1081 tgttcatcgt gacagccagg aacggcccgg acctaccggt cttcatcgac ggcaacaatg
     1141 tgggacgaac gaatcagctt ggcgtagcgc agatctcacg ctggggtgtt ccaggcacag
     1201 agtacgcggt cgaaatcgac acgcgggcag atccgcgtct tctgcctcgg tcccctgtgc
     1261 atgtcttcac actgcccgat gcgcacgaga tcttcgtggt gaacgagtca ttccagctcc
     1321 aggaacgtcg caggtctggg cacaggcggc gcgccagaat caccaaaatt gagtagtcgc
     1381 ccctgcgcgt actataaccg aacgcatggg agaccggacg gacgtcgtgg tcgtgggcgc
     1441 aggactctcg gggctttgtg ccgcgcgcaa gctgcgcgcc gagggcgcgt cagttctcgt
     1501 ggtcgaggcg agagaccgcg tagggggtcg tactcgcacc gagcagattg ggcagggtac
     1561 gttcgatctc ggcggccagt ggataggacc cggacacaaa cgtgttcatg cgcttgccaa
     1621 tgagctcggg attcaaacct tccccacgca caccgatggg aagaagattc tcgaggtcga
     1681 gggaaaggtc tcgacctaca agcgatcgat tccttccatg tcggtgccga acctcattca
     1741 gatgcaaggg gcgctcagtt atctcaagcg agttcggaaa cgaatttctc cgacggcgcc
     1801 catgagtgcc gaaggcgccg aggagctcga tgccgagacg ctcgaaacct ggcgatcacg
     1861 gttcgtgaag tcgagcaaga tcagcgctgt gatggatgct gcgattcgca cgattttcgg
     1921 cgcggaggcg cgcgacctgt ccgcgctcta ctttctcatg tatctcaatg ccgggggagg
     1981 cctgctgagc ctttcggaag tccgtggcgg tgcacagcaa gatcgcttcg ttctcggcgc
     2041 gcagtcgata tctcgcgcac ttgccgagga gctaggcgat gcgctgaagc tcggcgtagc
     2101 ggttcgcaag ttggttcaga gccaggatgg cgtagaggcc gtgtctgatg acgaaacgct
     2161 tggcggtcga tatgcgctcg ttgcagtgcc accaacgctt gcggggcgta tcgagtatca
     2221 accttcggtt tcggtcgggc gagatcagct cacgcagcgc ttcgcgatgg gcgccgctgt
     2281 gaaagtgctc gtgacctacg agcgcgcgtt ctggcgcgag gctggattct ctggcgaggt
     2341 cgtgagctcc gacggaccgc tcagtgtcgt ttacgacaac acttcgcacg atcggcagca
     2401 accggcgttg gtcggtttcg tggtggggag ccaggcccgt caatggtccg cgcagccctt
     2461 ggcggatcga aagcgtcgag tcgttggcgc tctcaaacgt tacttcggtg aagaggccga
     2521 atcccttcag gagtatcacg agctcgactg gggcgcggag ccttggtcac ggggttgtcc
     2581 tgtcggggga ttgccccctg gcatgctcac acactctttt gcgcacctcc ggaagcccga
     2641 agggcgaatt cattgggccg ggacggaagc ggcgaccgaa tggatcggct acatggaagg
     2701 cgcgattgaa tcaggcgaac gcgcggctga cgagatcctc cgtcgcctct aatcgcatca
     2761 ggcaagccgc cgagctcatg cactgagcgt cagcgacacg gtgacgtctt tggatgccgg
     2821 tgtgtagctg ccctctgcgt agttgttgag cgggaccagc acgttggctt cgggaaagta
     2881 ggtggcgacg cagcgcttcg ggatggggta gggaactacc gtaaagcgag gcgcgatgcg
     2941 cagcctgccg ccgaactcgt tggtgatgtc gactttcgca cctgcggaaa agccggcctc
     3001 cagcatgtcg ctttcgttca tcagaatcac gcgtcgtccg ccgcgaatcc cccggtatcg
     3061 gtcatcattc ccgtacaccg tggtgttgta ctgatcgtga gttcgcacgg tcatcatgcg
     3121 ataggatccg tcgggaaggt ggaggtccgg cgcctgattg atcgtgaact tggctttgcc
     3181 gctctcggtg ttgaacctgc gttcgcgcgc accgttatgg agatggaagc cgccgggctc
     3241 tcgaacgcgc tcgttgaagt cttcgaatcc ggggatcgtt tcggcgatta gatcgcgaat
     3301 ccgatcgtag tcctcgatga gccaacacca gctcacagga ttctcggggc cgagcacgcg
     3361 ctgcgcgagc caagcgacga tccctggctc gctgtagagt tgcgggtgtg caggcttgag
     3421 gtgccctcgt gaggagtgga ccacgcccat cgagttctcg acggtgacga actgcgggcc
     3481 gtaggcttgc tcgtcgatct cggaacgcgc gagacaaggg agaatgaacg tccatttgtt
     3541 gtgaacgaga tggctgcggt tgagcttcgt gctcacgtgg acccgcatct cgcaagcttc
     3601 gagtgcgtgc gcggttcgct ccgtgtccgg tgtcgcctgc aggaagttgc cgcccatcgc
     3661 gaagaacaat tttgcccgcc cgtcgagcat ggcgtgaatg gcctcgaccg tgttgaaacc
     3721 gtcttcacga ggcgggtcga atccgaacac ctcttcgatg cgatcgagca gcgccttcgg
     3781 tgggcggtca tgaatcccca tcgttcggtc gccttggacg ttgctgtggc cgcggacagg
     3841 gcacgtgcct gcgcctggaa cgccgatgct gccccgcatg agaaggaaat tgacgatctc
     3901 ccgaacgttc cccacgccgt tcttgtgctg cgtgagtccc atggcccagc agatgatcgt
     3961 cttacggctg cgaacgatga tttcgacggc tctttcgagc tcgcctcgtt cgatgccggc
     4021 cgcttcgatg agcgcgtcga tgtcctgaga ctcgaggtgg gcgcggagct cggcgaaacc
     4081 ttctgtgtag aggccgatga agtcctcatc caggacttcc ccggggcgct tcgcttcgag
     4141 ctccagcaac acgcacatca gcgctttcag caatgcaacg tcgccgttga tttgaacttg
     4201 caagaataag tcggcgattt gagtgccacc cgtcagaacc tggcttgggc tctttggatt
     4261 cacgaacccc attagccccg cttcagggag cggattgatc gcgatgatct tggcaccgtt
     4321 cttcttggcc ttctgcaggg ctgtcagcat ccgcggatgg ttcgtgcccg ggttctgccc
     4381 catgacgatg atcgtgtcgg cttcgtgaat gtcttcgagc gtgacggagc ccttgccgat
     4441 gccgacggtt tcaccgagac cgacgccgct cgactcgtgg cacatgttgg aacagtccgg
     4501 aaggttgttc gttccaaacg cacgcacgaa gagctggtat agaaacgcgg cttcgttgct
     4561 ggtgcgaccc gaggtgtaga agatcgcctc gttcggagaa tcgagtccct tgagtccggc
     4621 ggcgatgagg tcgaacgctt ccttccaatc gatcggttcg tagaagcgcg agccgggtcg
     4681 cagcatgagg ggttcggtga gcctgccctg cagaccgagc cagtagtcgg atcttgttgc
     4741 cagatcgtcg atgctgtatt gctggaagaa cttccaatcg aggcgacgcc gagtgctttc
     4801 ccaggcggcc gcctttgcgc cgttctcgca gaattcagtc agggcggctc gatcgtcggg
     4861 gtcgggccag gcgcaaccgg ggcagtcgaa tccgtcggtc tggttcatct gaccgaggat
     4921 gcggacggcg gagggtaagg agtgtccgtg cttcaacatg tgcgcgaacg ctgagctcac
     4981 cgacgcgagt cccgcggcct tggtttgggg ggcgccgacg cgaaggtcgt cttcagtctc
     5041 tgggggctcc gcgcggctcg gtcgctcacg tgtcatcaac cgacctcgac ctgggcgaac
     5101 aaggcctcgc ccgcctcctc ggcaatacgc tcaccccgcg agtaaacgtt gaatcggttc
     5161 tcgcgtgcga agccaatgag tgtcaggtcg aatcggcgcg cggtttcgat cgccaagctc
     5221 gatgcagcgc cgatggcgac cagtatcgga aaacgggctc gtaccgtctt ttgaaccagc
     5281 tcgaagcttg cgcggctgct cacgaccacg aacgcccccg gcggcgagca aaccgattgg
     5341 cggagccacg cgccaacgag cttgtccaga gcgttgtgtc gcccgacgtc ctcgcggtgg
     5401 agtagcagct cgccgctcgc gtcgaaaagt gcggctccgt gtgttccgcc agtgtgccga
     5461 aaccaggctt gagactcctc caaagagctc aaggcccggc gcacggccgc cgggctgaac
     5521 gcatcgcctt cgacggagat gcgcccgcat ccctcgctcg aaagcgattc gaccgtgcgt
     5581 tcgccacaca cgccgcaagc gctcgtcact gtgaggtttc tttcgagcaa ccgggacgga
     5641 accacgacgt tctctctcag gaccgcgcga accacgttgt ggggcgtttc ggaccgagcg
     5701 cagtggcgaa cgctgagcag atcggagatg ccggcgacga caccctcggc atagagaaac
     5761 ccagccacga ggtcatggtc gtgcccgggc gtacgcatgg tcatcgccag cggtgtctcg
     5821 cggcgccgat gtgcctcacc gtactcgagg atgatctgaa gtggttcctc gacggcgacc
     5881 aggtcgctac aaacctcccg gcgctcgcgt tcgattcgct cgaccttcgt gcttgcaacc
     5941 ggggtccgtg ccatgaccgg gcaagcgtac agtgcatcca gctttcgaaa aaggggacgg
     6001 ccgccttttt gaagccgttg gctagtagat gcccgggacg atccggtacc ggaccttttc
     6061 acagtagcgg gtccagtcag cgccgtactt cttggagcag aagttgtggt cccgccgttc
     6121 gcggtgaatg agcaggatcg cgaagtagat tgggtagaag tagggaagtg ccgagccgaa
     6181 gaggcaaggg agtgaccagg acagggccat caggatgtcg ccgacgtagt tgaaatgtcg
     6241 tgaccaaccc cagaaacctg acagcaacaa tttaccgcct tgctccgtct ggatgtactc
     6301 gaccttcttt ccccagattc gggcgttctc cgggtctttg cggaacttgt gcttttgcag
     6361 attgaccact cggaagatgt agaggccgag catgttcacg ccgatgatca agacggcgcc
     6421 ccaccaggga agcgaatgaa ctcggtcgat caggtagtgg gcctgtagcg agtacgtcat
     6481 cggaacccag accaagtcac caaacgcgag catgtagccg aagttttcgt gcttgatgtc
     6541 catcgtggtg aggatggcct cttcgttcca gaagtagtcg atgatgtaga acatctggaa
     6601 gatccaaacg agaatcatcg aggttgaaac aaacccgtac tcttcgtact gaacgtaggc
     6661 gaacgaagtg ttgatcagca cccagagaat caacccgggt ctcgcttcac aaaagatctt
     6721 caagtcgagg ccgtctcgac cgggcgggac gcgcgggttg tggcccgtgc ccatccaaaa
     6781 atcgtgaatg aacttgccgc tcagatgtcg cgactgccca tgccgaaggc cgtagctgta
     6841 gagaaacgcg ctgaacgcga agacgaagat cgtcattacg ctgatcaagg caccgaactc
     6901 gtcatggagc tcgcgaatcg aaaacaaccc cgaccagtgc aggatggcga cggcgatgaa
     6961 ggtcgccagc agcgagaaca gaccattcat tcgatacgga agacggcccc catcggggag
     7021 ctcggcaccg aggacctcgc ggcccggcgc aaaccgctga aggagcgcct ggaagacgaa
     7081 ccagactccg tagatctcgg ctgcccgcca actgggcatc atcgcatctc ggaatccctc
     7141 ccaatcggcc cctgcgccgg gaagcaccgc tccatcgttg aagcgcaccg cgaagaagag
     7201 ataggccgtg aagatgggga gcccgaaaat catcgcgaag tttccgggta ccccgccgaa
     7261 ctcaagcttc tgcttctgct gattcatcgg cgcgactcta aggcaaatcg gcgctggcgc
     7321 gacagcaagt gcagaatttc gcccagaagc ggccgcaaga ggcggatttt tggccaaatt
     7381 gggccacgta cacgaggagt ttccccagct gttgcggcgt gcgactgcgc tatgctcgcc
     7441 tgggcccttc gctcgatgat catcgttaca ggaaccaaac gctccggcac ctcgatgtgg
     7501 atgcagcttc tgagcgctgc tggctttccg ccgattggag acgcctttcc acgcaactgg
     7561 gagaccacgc tgaaggacgc gaacccggga ggcttctggg agtccgagct gcgacggggg
     7621 atcttccatg cgaccaatcc ggatccgaag acggggacct atctttttcc cgaacagacc
     7681 gcccggcacg ccgtcaaggt gttcatcccg ggactgatca agaccgatcg cgctttcatc
     7741 gggaaggtcg tcgccaccat tcgtccatgg cgtcagtatg tccgctcggt gaatcggctc
     7801 tatgcgatgg agcgcgaggc ccaagaggcg gcacgaaagg acccggaaga acgcttgccg
     7861 cctgcggttt tcatgtctcc agtcatcgaa tggtgggtgg agaatttttc cttgttcagc
     7921 gacatcgtga cgaggcgcta tccattctat atggtcgcct acgactcggt gcttgatcag
     7981 cgcgaggaga ccttacgctc tgtttttgga tggctcggag acggcgacgt cgatgcggcc
     8041 attcggcacg tggagcccgc gctccgaacc caagaccgag cgaacgatga tcaggtcagc
     8101 gacgacgagc agggcatcga gcccgaggcc atcgccgtct tcgacgcgct ctacgaagtg
     8161 gttctgaggc aagctcccct cgagcaggcg ttcgtagacc gcctgaatgc agccaacgag
     8221 ctactcaagc ccaggatcag cgagacgatc aagcgggtgg ctcaggcccg gctcgaacgg
     8281 aggcgcttca tcgaacagcg acgcgccagc aagacgcagt tatagtgcgg ggcaatgcgg
     8341 cttaggcgga aagtctgcct gttgggcgct cccggggtag ggaagacgag cctcgtcagg
     8401 cgattcgtcg agcagcggtt cgacgacgac catgactcca gtatctcgat ggcgatctcc
     8461 aagggcgagt tgcagctcgg cgaagtctcg ctcgagatga tgctctggga tccgggtggt
     8521 gatgaggcct ccgcgcagta tcaccgctct ttcatatccg gagcttcagg cctcgtattc
     8581 gtcgcagatg ccacgaagcc acgaacgctc gaccacttgt tggaggcgca aacca
//
LOCUS       DASRZF010000436         2052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2403667, whole genome shotgun sequence.
ACCESSION   DASRZF010000436 DASRZF010000000
VERSION     DASRZF010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2403667"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="VFG22_19155"
     CDS             <1..1098
                     /locus_tag="VFG22_19155"
                     /inference="COORDINATES: protein motif:HMM:NF015410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HET6418424.1"
                     /translation="VVGIVSEHDILKTQGSHPTVILSEIGRAKTQERLRALRDQAEEL
                     LRQYLEDEVGMGFLSNMISEINDTLIRRAIQLSLSALSEQGKEPPVPFCWLALGSEGR
                     EEQLLRTDQDNAILYADPGDDDGAAEEFFLDLGKRIVDILVAAGFERCPGEVMASNPK
                     WNQPLSVWRQYFFDWVREPENVAVMHTNIFFDFRPVFGDDALAAELKAYIFERIKDDR
                     SFLAFFARNATQNPPPLSFFRNMVVERSGEHRDAFDIKARAMMPLADAARVLVYDLGI
                     NIYGSTAERWERIAETDENLARLAGEAGMAYEILMRIRALEGLNRGSSGRYVHIKELN
                     KIERQTLRSTFSVVKDVQLMLTSRYRIDFLR"
     gene            1106..1822
                     /locus_tag="VFG22_19160"
     CDS             1106..1822
                     /locus_tag="VFG22_19160"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HET6418425.1"
                     /translation="MLLDFIKRLIPGIPSASKSKDWPEPLREYMSKRTSPPKDKPLTD
                     VRFVVFDTETTGLDLAKNRLLSIAGVGMHGPEIQLDDAFDSMVTQSDVGGSAAAVIHG
                     LISNDLTGGLPEELAAAQFLAFAGSAVLVAHHAAFDLRMLQKAIAPYRGAKIWSPAVD
                     TAQLAQRLEDGLMSSNRAQGADQRKAYQLDSLVERYGIDVPERHTAAGDALATALLFQ
                     RLLKQAGRRGIRTLGDLLAH"
     gene            complement(1836..>2052)
                     /locus_tag="VFG22_19165"
     CDS             complement(1836..>2052)
                     /locus_tag="VFG22_19165"
                     /inference="COORDINATES: protein motif:HMM:NF018657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
                     /protein_id="HET6418426.1"
                     /translation="EQLYWNGAPLEGMYPLSIVTHGQALNITVTSCGESLDFGIVACR
                     RSVPSAQRLIDHMDAALVELEQRVERS"
BASE COUNT          414 a          637 c          615 g          386 t
ORIGIN      
        1 gtggttggaa tcgtctcgga gcacgacatc ctcaagacgc agggcagtca ccccaccgtg
       61 attctttcgg agattggccg tgcgaagacc caagagcggc tccgggcgct tcgcgaccag
      121 gcagaggagc tgctccgtca gtacctcgaa gacgaagtgg gcatgggatt tctctccaac
      181 atgatctcgg agatcaacga caccctcatt cgacgcgcca ttcaattgtc gctttcggcg
      241 ctctcggagc agggcaagga gccgccggtc ccgttttgct ggttggcact cggtagcgag
      301 ggtcgcgagg aacagctcct tcgaacagac caggacaatg ccatcctgta cgcggacccc
      361 ggcgatgacg acggcgctgc agaggagttc ttcctcgatc tcggaaagcg gatcgtggac
      421 atcttggtgg cagccggctt cgaacggtgc ccaggcgagg tcatggcgag caaccccaag
      481 tggaaccaac cgctctccgt ctggcggcag tacttcttcg actgggttcg cgagccggag
      541 aacgtcgcgg tcatgcacac gaacatcttt ttcgactttc gtcctgtctt tggcgacgac
      601 gcgctggccg cagagctaaa ggcgtacatc ttcgagagaa tcaaggacga tcgctcgttt
      661 ctcgcgttct tcgcgagaaa cgccacccaa aacccaccac cgctcagctt tttccgcaac
      721 atggtcgttg aacggagcgg cgagcatcgc gacgcgttcg acatcaaggc ccgcgccatg
      781 atgccgttgg ctgatgccgc acgcgtcttg gtctacgatc tcggcatcaa catctacggg
      841 agcacggcgg agcgctggga gcgaatcgca gaaacggacg aaaatctcgc acgcctcgcc
      901 ggcgaagcgg gaatggcgta cgaaatccta atgcgcattc gcgcgctcga aggactcaat
      961 cgcggctctt cgggtaggta cgtgcacatc aaggagctca acaagatcga gcggcagacg
     1021 ctccgaagca cattctcagt cgtcaaggac gtgcagttga tgttgacctc gcgctaccgc
     1081 atcgatttcc tacgctgagg ctcgcgtgct cctggacttc atcaaacgcc tgattcccgg
     1141 aatcccgtcg gcatccaaat cgaaagactg gcctgagcct ctgcgcgagt acatgtcgaa
     1201 acgaacgagc ccaccaaagg acaaaccctt gacggacgtc cgcttcgtcg tcttcgacac
     1261 cgaaacaacc ggtctcgatc tcgccaaaaa ccgccttctg tcgatcgctg gtgtcggcat
     1321 gcacggtccg gaaatccaac tcgacgatgc gttcgattcc atggtgactc agtccgacgt
     1381 ggggggctcc gccgccgccg tgattcatgg cctgatctcg aacgacctga cgggcggtct
     1441 gcctgaagag ctggcggccg cccagttcct cgcgtttgcg ggcagtgcgg ttctggtggc
     1501 gcatcacgcg gctttcgacc tgcgcatgtt gcaaaaagcc atcgcgccgt accggggcgc
     1561 caagatctgg agccctgcag tcgacacagc tcagctcgcg cagcggctgg aagacggcct
     1621 gatgagctcg aaccgagcgc agggtgcgga tcaacgaaaa gcgtaccaac tcgacagtct
     1681 cgtggagcgc tatggcatcg atgtgccgga gcgacacacc gccgcaggtg atgccctcgc
     1741 aaccgcgctc ctgtttcaac gcttgctgaa gcaggcgggg cgaaggggaa tccggactct
     1801 tggcgacctc ttggcccact gaatgcgaca cacattcacg atcgctctac tcgctgctcg
     1861 agctctacga gcgccgcgtc catatggtcg atcaggcgtt gtgcgctcgg gacagatcgg
     1921 cggcaggcca cgattccgaa gtcgaggctt tcgccacagc tcgtcacggt gatgttcagc
     1981 gcctggccgt gcgtcacgat ggagaggggg tacatgccct cgagcggcgc gccgttccag
     2041 tagagctgct cc
//
LOCUS       DASRZF010000437         7199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_1029318, whole genome shotgun sequence.
ACCESSION   DASRZF010000437 DASRZF010000000
VERSION     DASRZF010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 7199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7199
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1029318"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            479..1516
                     /gene="glnII"
                     /locus_tag="VFG22_19170"
     CDS             479..1516
                     /gene="glnII"
                     /locus_tag="VFG22_19170"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF041605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase GlnII"
                     /protein_id="HET6418427.1"
                     /translation="MSIIKAEYIWIDGTEPTPLLRSKTKMLEANIIEKVLGAITGVAA
                     LPNWGFDGSSTNQATGDKSDCQLFPVFACRDPLRGGDNLLVLCEVVDSTGKPHPTNTR
                     RAAAEIEERVEALDCWYGMEQEYTMMKDNWPLGFPEGGYPGPQGPYYCGVGSKYIFGR
                     EIVEEHMDACIKAGIKISGINAEVMPGQWEFQIGPAGITEVGDHMYMARYLLGRIAET
                     YGVDISYDAKPTKGDWNGAGCHTNFSTKGMREEGGLKLINEYCEKLGKPERIALHLKN
                     YGAGLEDRLTGAHETASYKEFKYGVSDRGASIRIPLQTSLDGKGYLEDRRPCANIDPY
                     TVARLLAEVAE"
     gene            complement(1572..2576)
                     /locus_tag="VFG22_19175"
     CDS             complement(1572..2576)
                     /locus_tag="VFG22_19175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HET6418428.1"
                     /translation="MRAVLLDTPAPIETHPLRLGDVPIPVPAQDEVLVKVRACGVCRT
                     DLHVVEGDLDVRRSPIIPGHQVVGEIEALGSDVGDRAMGERVGVAWLHRTCGCCSFCT
                     SRRENLCDAPEFTGWTVDGGFAEYVVAPARFTYPLPDGFSDLQAAPLLCAGIIGYRCL
                     TQTGVRDWSGARIGLYGFGAAGHVAIQIARARGAEVYVCTRDREKHQALAEELGAVWT
                     GGSTERPPVSLDAGIIFAPAGEIVPAALSHLDKGGTLVLGGIHMSPIPELRYELLYGE
                     RVVRSVANNTREDGHAFLREAAAVGVRSYTETFPLEEANEALERLQHDGIRGAAVLTL
                     "
     gene            2575..3591
                     /locus_tag="VFG22_19180"
     CDS             2575..3591
                     /locus_tag="VFG22_19180"
                     /inference="COORDINATES: protein motif:HMM:NF026066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418429.1"
                     /translation="MEPTLAPALTLALRPGRAPALRLALASFYWLHSHVDSRPSVVTN
                     AQIADILDAVADRLEGQHANIYRVDAYRAGAAVVRAHPDSIQEVALHEGREGLDALPQ
                     IGSSLASSIDEIAHTGRLGMLERIDGRMSPVELFKTVPGIGEELAERIHRELDIDTLE
                     ALELAAHDGRLDAVAGFGPRRLEGIRDILATLLTRSSRGRARRFEELRHAGASVVPPE
                     TAEVGPDVSLLLEVDRDYREKAEADELPRIAPKRNNPEGKAWLPILHRYEDGWHLTAL
                     YSNTDRAHELGRVRDWVVVYFERDGQEGQCTVVTEFRGPLTGKRVVRGREDEVAALDT
                     SPSQ"
     gene            complement(3585..4292)
                     /locus_tag="VFG22_19185"
     CDS             complement(3585..4292)
                     /locus_tag="VFG22_19185"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418430.1"
                     /translation="MREAEFGAIKVRLTGGEDREAGGDGPLVVLMHGFGAPGTDLVGL
                     WRVLDVPRSMRFAFPEAPNEVPGMPGARAWWMLDLARAEQAMSQGPRNYAAEVPPGME
                     EATDRVVKMLEAMQTELGVPDRRLIVGGFSQGSMAACNAVFTRSVSPAGLVILSGTPV
                     NLAAWKVGMPERQGMTVFQSHGERDPLLSFEAAEQLHDALGEAGLLTDWVQFRGGHEL
                     PMPVLEGLGRFLSTTGY"
     gene            complement(4304..5593)
                     /locus_tag="VFG22_19190"
     CDS             complement(4304..5593)
                     /locus_tag="VFG22_19190"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HET6418431.1"
                     /translation="MNDKATLEYGDESYDMAIVEGTYGEQAFDIRSLRKDTGLITLDS
                     GYMNTGATTSEITYINGEEGILLYRGYPIEQLAEASTFVETSYLLIYGELPTPSQLQS
                     FSNLLTYHSMLHEDMRHLFDGFPSNAHPMAILSSMVSALSAYYPDSLTPDTHNQHHIA
                     RVLSKVRTIAAFTYQKQLGRPVAYPKNELKYCANFLYMMFSHPAEPEYQPDPELVRAL
                     NVLLILHADHEQNCSTSTVRLVGSSRANLYAAISAGISALWGPLHGGANQAVIEMLEE
                     IRAEGGDSTAFMSQVKNRERRLMGFGHRVYKNFDPRASILKTYADKLLDHLKIEDPLF
                     DIARQLEEVALSDEFFIERKLYPNVDFYSGIIYRAMGIPTEMFTVLFALGRIPGWIAH
                     WKEMMEDPDLRIGRPRQVYTGAKSRDYIPMGQRSAQG"
     gene            complement(5682..6431)
                     /locus_tag="VFG22_19195"
     CDS             complement(5682..6431)
                     /locus_tag="VFG22_19195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418432.1"
                     /translation="MARGNLNRGMGIALGFIAIACGSETSSEPPSERSPDALATALTP
                     QQAPEYYVEQANKYFDTLDTSADRDSVPEYSVRVARWELPPWLWLTGYERENMIVTTN
                     AALALDPSTVPVRDCRAFSVQPFARCYVTFEYAEGSCPIYEEFVFNDQGEMTFIEAWS
                     DQPGYLPMADPSDRWAEGPDVYRLSTKVPGLGNELGLIDLESAGMQEAAAADAELADF
                     VRRAEDFWPAWLEASAAAGPDYFARGCGWID"
     gene            complement(6493..6933)
                     /locus_tag="VFG22_19200"
     CDS             complement(6493..6933)
                     /locus_tag="VFG22_19200"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET6418433.1"
                     /translation="MIQYENSIVIKAPVNEVFAYADDFLTMPDWLPGLVEVHNVIGAG
                     EGRQCDWTFKMVGVLLRGQAVVVDCVPNERSTHQTIGMLEATWTSIVEPHEDGTKLII
                     EVEYAIPVPVLGRLAEHLTVRRMERDLESALLNVKDIIESRSVA"
BASE COUNT         1407 a         2267 c         2197 g         1328 t
ORIGIN      
        1 ccgcaagcgg cgagcatcgc cagtccagcc aagcccacga atacaatcga aagccaatta
       61 cgcattacga actccccaac atgacaaagc caactatgtc aacattcttc actggctctg
      121 tacgtcccgc aaatgccaag aggccaatgg catagacgat tatctattag tttcgatatg
      181 ttatggagat tcacgctccg gccctcacga gctctactct tacgcgcctg gacggaatca
      241 ccaagaaata gtggatgccc ccgtttccgg cccgcaagta ccgagcccca cagcgctctg
      301 ctctcgcgtt ttttcacgta ggaaagggct cggaaacctc ttcgaaacaa tagcggggca
      361 gagatccggc aacccactcc tcggcgcggg attcaccggt cgggagtcga gcccgctgtc
      421 cacccccgac tccgttcgtg ggtgccccag cgacgagaac atctttagga gtagtacgat
      481 gagcatcatc aaggctgaat acatctggat cgacggcacc gagccgacgc cgcttcttcg
      541 ctcgaagacg aaaatgctcg aggcgaacat catcgagaag gtcctcggtg cgatcaccgg
      601 tgtggccgca ctgccgaact ggggcttcga tggttcgagc accaatcagg ccactggcga
      661 caagtcggac tgccagttgt tcccggtgtt tgcctgtcga gatccgctcc gcggcggtga
      721 caatttgctc gttctctgcg aggtcgtgga cagcactggc aagccgcacc cgaccaacac
      781 gcgtcgtgca gccgccgaga tcgaggagcg ggtggaggcg ctcgattgct ggtacggcat
      841 ggagcaggaa tacacgatga tgaaagacaa ctggccgctc ggtttccccg agggcggcta
      901 tcccggtccg caaggtccgt actactgcgg cgtcggatcg aagtacatct tcggccgcga
      961 aatcgtggaa gagcacatgg acgcgtgcat caaggccggg atcaagatct cgggcatcaa
     1021 cgccgaggtc atgcccgggc agtgggagtt tcagatcgga cccgcgggga tcaccgaggt
     1081 cggggaccac atgtacatgg ctcgctacct gcttggtcgc atcgcggaga cctacggcgt
     1141 cgacatctcc tacgatgcga agcccacgaa gggcgactgg aacggcgcgg gctgccacac
     1201 caacttcagc accaaaggga tgcgcgagga gggtggattg aaactcatca acgagtactg
     1261 cgagaagctc ggcaagccgg agcgcatcgc ccttcatctc aagaactacg gcgccggcct
     1321 cgaggatcgt ctcacgggag cccacgaaac ggcgtcctac aaggagttca aatacggcgt
     1381 gtcggatcgc ggcgcttcca tccgcatccc cttgcagacc tccctcgacg gcaagggcta
     1441 cctcgaggat cgtcgcccat gcgcgaacat cgatccttac acggtggcac gcctgttggc
     1501 agaggtcgcc gagtagagcg actgcgtttc caagcgaagg cgccgcgttc ctcggagcgc
     1561 ggcgtttttc gttaaagcgt caacacagcg gcgccacgga tgccgtcatg ttgcaagcgc
     1621 tccagcgctt cgttggcttc ctcgagcggg aaggtctcgg tgtagctgcg gactccgacc
     1681 gccgcggcct cccggagaaa tgcgtgtccg tcttcgcgcg tgttgttggc tacgcttcgg
     1741 acgacacgct cgccgtagag aagctcgtag cggagctcag gaatggggct catgtggatg
     1801 ccgccgagca cgagcgtgcc gcccttgtcg agatgggaga gggccgcggg gacgatctca
     1861 cccgctggcg cgaaaatgat ccccgcatcg agtgagacgg gaggcctctc ggtgctgccg
     1921 ccagtccaga ccgcgccgag ctcttcggca agcgcttggt gcttttcgcg atcccgggtg
     1981 cagacataga cctcggcgcc ccgtgcgcgt gcgatctgga tcgccacgtg acccgccgcg
     2041 ccaaaaccat agagaccaat gcgcgcgccc gaccagtctc gaacaccggt ctgcgtcaag
     2101 cagcgatagc cgatgatgcc cgcgcaaagg aggggagcgg cttgaaggtc ggagaagcca
     2161 tcgggcaagg gatacgtgaa gcgcgcgggc gccacgacgt actcggcgaa tccgccgtca
     2221 accgtccagc ctgtgaactc cggggcgtcg cagaggttct cgcgacggct cgtgcagaaa
     2281 gagcagcagc cgcaggtccg atgaagccac gcaacgccga cccgctcgcc catcgcacga
     2341 tctcctacat ctgagcccag agcttcgatc tcgccgacga cctggtgacc ggggatgatc
     2401 ggcgaacgcc tcacgtccaa atcgccttcg accacgtgca gatcggtgcg acaaacgccg
     2461 caggcccgca ctttgacgag gacctcgtcc tgcgctggca cggggatcgg cacatcgccg
     2521 aggcgcaagg ggtgtgtctc gatcggtgca ggggtgtcga ggagaaccgc gcgcatggag
     2581 ccgactctag caccggcgct cacgctcgca ctgcgccctg ggagagcccc tgcccttcgt
     2641 cttgcgctgg cgtcgttcta ctggcttcat tcgcatgtgg actcgagacc ctcagtcgtc
     2701 acgaatgctc agatcgcaga cattctcgat gctgtcgccg accgcctaga ggggcaacac
     2761 gcgaatatct atcgagtcga cgcgtaccgt gccggagccg cggtggttcg agctcacccc
     2821 gatagcattc aagaggttgc gctacacgag ggacgcgaag ggctcgacgc gctgccgcag
     2881 atcggttcga gtcttgcgag ctcgatcgac gaaatcgcgc atacgggcag gctcggaatg
     2941 ctcgagcgga tcgatggcag gatgtctccc gtcgagttgt tcaagacggt tccgggcatc
     3001 ggcgaagagc tggcggagcg aatccatcgt gagctcgaca tcgacaccct cgaagcgctg
     3061 gagctcgcgg ctcacgacgg ccgcctcgat gcggtggctg gcttcggacc gcgtcgtctc
     3121 gaagggattc gggacatctt ggcgacgctg ctgactcgct cttcgcgcgg cagagcgcgg
     3181 cggttcgaag agctccggca tgcgggcgcg agcgtcgtcc cgcccgagac cgccgaagtc
     3241 ggaccggatg tcagtctgct gctcgaggtc gatcgggact atcgcgagaa agccgaagca
     3301 gacgagctcc ctcgaatcgc acccaagcgg aacaaccccg aagggaaagc ctggttgccg
     3361 atccttcacc gttacgagga cggctggcac ctcacggccc tctactcgaa taccgaccgg
     3421 gcccacgagc tcggacgcgt gcgagactgg gtggttgttt acttcgagcg ggacggtcaa
     3481 gaaggacagt gcacggtggt caccgaattc cgcgggccct tgaccggaaa gcgcgtcgtt
     3541 cgcgggcggg aagatgaagt ggcggcgctc gacacttcgc cgagtcagta gccggtggtc
     3601 gacaagaaac ggccgagccc ttcgagcact ggcatcggaa gctcgtgtcc gcctcgaaac
     3661 tgcacccagt cggtcaaaag accggcctcg ccgagcgcgt cgtgaagctg ctccgcggct
     3721 tcgaacgaaa ggagcggatc acgctcacca tggctttgaa agacggtcat tccctgcctc
     3781 tcgggcatcc cgactttcca tgccgcgaga ttcaccggcg tgcccgaaag aatcaccaag
     3841 cctgccggtg acacgcttcg ggtgaacact gcattgcaag ccgccatcga accctgcgag
     3901 aagccgccca cgatcaggcg ccggtcggga acaccgagct cggtttgcat cgcctcgagc
     3961 atcttcacga cccgatcggt cgcttcttcc attcctgggg gaacctctgc cgcgtagttt
     4021 cgcggcccct gcgacatcgc ctgctctgca cgcgcgagat cgagcatcca ccaagcgcgt
     4081 gcgcctggca tcccaggaac ttcgttcggg gcctctggaa atgcgaacct catgcttcgc
     4141 ggcacatcga gaacccgcca tagaccgact agatcggttc ccggcgcgcc gaagccgtgc
     4201 atcaggacga ccagaggccc gtcaccgccc gcttcgcggt cctcgcctcc cgtgagacgg
     4261 accttgatgg ctccgaactc tgcttcgcgc atttgaaccg aacctagccc tgagctgagc
     4321 gctgtcccat tgggatgtaa tcccgactct tcgctccggt gtagacctgc cgcggacgac
     4381 cgattcgcag atccggatct tccatcattt ctttccagtg tgcgatccag ccggggatcc
     4441 gaccgagggc aaagagcacg gtgaacatct cggtcggaat tcccatcgcg cggtagatga
     4501 tgccgctgta gaaatcgacg ttggggtaga gcttacgctc gatgaagaac tcgtcggaca
     4561 gggccacctc ttcgagctgg cgcgcgatgt cgaagagcgg gtcttcgatc ttcagatggt
     4621 ccagcagctt gtctgcgtag gtcttgagga tcgacgcgcg tgggtcgaag ttcttgtaca
     4681 cgcggtgtcc gaatcccatc aggcggcgct cccggttctt cacttggctc atgaacgccg
     4741 tgctgtcgcc gccctctgcg cggatctctt cgagcatctc gatcaccgcc tggttggctc
     4801 caccgtgaag cgggccccac agagcggaga tgcctgcgct gatcgccgcg tagaggttcg
     4861 ctcggctgga acccaccaag cggaccgtcg acgtagagca gttctgctcg tgatccgcgt
     4921 gcagaatcaa cagaacgttg agcgctctaa cgagctccgg atcgggttgg tactccggtt
     4981 ccgccggatg tgagaacatc atgtacagga agttcgcaca gtatttcagc tcgttcttcg
     5041 gataggcaac cggcctcccc agctgcttct ggtaggtgaa cgctgcgatg gttctaacct
     5101 tcgagagaac acgcgcgatg tgatgttggt tgtgcgtgtc gggcgtgagg ctgtccgggt
     5161 agtaagcgga aagcgcgctc accatcgaag agagaatcgc catcggatgc gcgttcgaag
     5221 gaaagccgtc gaagaggtgg cgcatgtctt cgtgcaacat gctgtgatac gtcagcagat
     5281 tcgaaaacga ttgcaactgc gacggggtgg gcaactctcc gtagatgagc aggtagctcg
     5341 tttcgacgaa cgtcgacgct tcagccaact gctcgatggg gtatccccga tagagcagaa
     5401 tgccttcttc accgttgatg taggtgattt cgctcgtggt cgcgccggtg ttcatgtacc
     5461 cggagtcgag cgtgatgagg cccgtatcct tacgcaggct ccgtatgtcg aacgcttgct
     5521 cgccataggt gccttcgacg atcgccatgt cgtagctctc gtcgccatac tccagagtcg
     5581 ctttgtcgtt catgccttcc ctccggcttg cgccgcgagc ttagcgtcca aagggtaata
     5641 agaccaacgt caatgagcct tcgaacccgg tctgccgctg ttcaatcgat ccaaccacat
     5701 ccacgggcga agtagtcggg ccccgctgcc gcgctggcct ctagccaggc aggccagaaa
     5761 tcctccgcgc gccgcacgaa gtcggcaagc tccgcgtccg cggcggcggc ctcttgcatc
     5821 cccgcggact cgaggtcgat gaggccgagc tcattaccaa gaccaggaac cttggtcgag
     5881 agacggtaca cgtcggggcc ttcggcccag cggtcgctcg ggtccgccat tggcaggtaa
     5941 cccggctggt ccgaccaagc ttcgatgaaa gtcatctcgc cctgatcgtt gaacacgaac
     6001 tcctcgtaga tcggacagga accctctgcg tactcgaagg tcacgtagca acgggcgaac
     6061 ggctgaacgg agaacgcccg gcaatcccga acgggaaccg tcgacgggtc caacgcgagc
     6121 gcggcgttcg tggttacaat catgttctcg cgctcgtacc cggtgagcca cagccaggga
     6181 gggagctccc atcgggcaac ccgcaccgag tactcgggaa cactgtcccg gtccgcgctg
     6241 gtgtcgagcg tgtcgaagta cttgttcgcc tgctcgacgt agtactcggg cgcttgctga
     6301 ggcgtcaacg cagtcgcgag cgcatcgggt gagcgctcgg aaggaggctc tgagctcgtt
     6361 tccgagccgc aggcaatcgc gatgaatccc aaagcgattc ccatgcctcg attgaggtta
     6421 ccgcgtgcca tcgcggctga ggatagtcgc ggacttgttc cgagcaagtc gggttggcct
     6481 cgcccgtcgg tatcaagcta ccgatctgct ctcgattatg tccttgacgt tcaagagcgc
     6541 tgactcgagg tctcgctcca tgcggcggac cgtcaggtgc tctgccagcc gcccgagcac
     6601 ggggaccggg atcgcgtact cgacttcgat gatcagcttg gtgccgtctt cgtggggctc
     6661 gacgatgctc gtccaagtcg cttcgagcat cccaatggtc tgatgcgtag accgctcgtt
     6721 cgggacgcaa tcgacgacga cggcctgacc gcgaagcagg actccgacca tcttaaaggt
     6781 ccaatcgcat tgccgcccct cgccagcacc aatcacattg tgaacctcga ccagccctgg
     6841 aagccaatcg ggcatcgtga ggaagtcgtc cgcatatgcg aagacttcgt tcactggcgc
     6901 tttgatcacg atactattct catattggat catacgaaga cgcagccccc tctagcctga
     6961 acgtagaata gtgccaccag atcccgaaca acaaccgacc ccacatcggc attccacatt
     7021 ggccgcaagg gtgaccgcag cgcgcggccc ggccggaccg agacgcatcg ggccgccgct
     7081 gaccatcctg gccgcggcgt tgctcgcaat gctcttcctc tatgcgccgg tccgtgactt
     7141 cggcttcgtc tgggacgacg tggcggaggt gcagcgcaat ccccttcttc acggggctc
//
LOCUS       DASRZF010000438         5826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2404302, whole genome shotgun sequence.
ACCESSION   DASRZF010000438 DASRZF010000000
VERSION     DASRZF010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 5826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5826
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2404302"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(570..2600)
                     /locus_tag="VFG22_19205"
     CDS             complement(570..2600)
                     /locus_tag="VFG22_19205"
                     /inference="COORDINATES: protein motif:HMM:NF013148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium bicarbonate transporter family protein"
                     /protein_id="HET6418434.1"
                     /translation="MSLQKRLHDSEILLDLAARDLQGLVQQALDALVDLGRLSIRQRA
                     AIQDTLGARGEGELHDLGSGVGVLRVRYEAQNGDACRCALIRVPEGVRASDHERVHYV
                     WLIVAPYGGSTPSNEDLEPFGWMLHDERFSASIIGANDPMQVLATYQLYLEYVEAPPD
                     QRRPSLLPTYPSVPPPQTGLASGLIADVRRKLPFYRGDFVDGFNVKGLAAILFLYFAC
                     LAPSIAFGGLLAFLTHGEIGVVEALLATAIGGVTYALFSGQPLTLLGSTGPVTIFLAL
                     LYVLCGQLGVPFLPGLFWIGIWTAAFMMFLALTNASRYIRYFTRFTDEIFAALIALIF
                     ITEALRDIFADIFDAEVPTNGELLALILALGTFMIAQQLSRVRQKPLLTKTVREFLAD
                     FGPAIAIFTMTAAAWAMRPIQLEHLAVPHEFATTSGRAWLVNPMDAPSWFWIASIPIA
                     VLATVLLFLDQNITVRLVNSPQHRLKKGEGYNLDMAVIAVLVAACSTIGLPWVVAATV
                     RSLNHVRSLATVRHHARGEHIISVRENRFTPLMVHLLIGGSLVFLGVLREIPMSVVFG
                     LFLFMGIASLRSNQFVDRLKLWITDPTLYPPTHYIRRVSRTVLHGFTLIQVACLSVLW
                     IVKESSFGVLFPLFIALLVPFRYGLERIFNQRDLAFLDADENPEEEQYREMD"
     gene            complement(2684..4021)
                     /locus_tag="VFG22_19210"
     CDS             complement(2684..4021)
                     /locus_tag="VFG22_19210"
                     /inference="COORDINATES: protein motif:HMM:NF005913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET6418435.1"
                     /translation="MTEDDPELAEHAFRLCQQLLRIDTTNPPGNELPAAELLAEELEG
                     AGLEPLVMQSAPGRGNVVARLRGTGEKPPLLLTAHLDVVEADPSAWDHPPFCGEVHDD
                     CLWGRGAVDMKNMAAMSVAILTRLAREGVKPKRDLIFAGVADEEAGCRYGSLWLCENH
                     ADLVRSEFAIGEGGGFNIQVAGRSFFTVQVAEKGVCWVRARAIGEPGHGSMPREDSAV
                     IQLSEAIARLGKEGLPRHSSAPVQGFIAALASHLPAPIRPAIKLLASPTFGPAMLNIL
                     PDPSVRRAFRALLGNTASPTVLRAGNKTNVIPGLAEAEIDGRILPGQSRDDFMRELGD
                     VLGTGIALEIMHSLPPVVTEPKESSLYSLIHEVMSERAPSSPIVPFIMQGFTDAKAFT
                     SIGAKWYGFAPVKLPPSLRFADMFHGHNERIPVDGLKWGTHTLMQVVRRSIGA"
     gene            4162..4536
                     /locus_tag="VFG22_19215"
     CDS             4162..4536
                     /locus_tag="VFG22_19215"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HET6418436.1"
                     /translation="MSLFEQLGGEPALRAIIDDFVDRCFDDMMIGFLFARAERERIKR
                     FEYEHAAQHLGAPVEYHGRPLDEAHGPHRIFGGQFDRRRQILIETLKDHDASAEVVDA
                     WISHQDTLRTLITRDPDSNCQG"
     gene            4539..5450
                     /gene="rsgA"
                     /locus_tag="VFG22_19220"
     CDS             4539..5450
                     /gene="rsgA"
                     /locus_tag="VFG22_19220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HET6418437.1"
                     /translation="MVETIEGLVVRSTAGFIDVQVDGEVLQARIRGRLKNTARRTTDL
                     CVIGDRVRVSCPDERSYVVSEVMPRKTRFSRRQPGRGPNKEDVLVANLDQLLITFCYG
                     PPGLKTRLLDRFLVIAEHQDVHPVILMNKSDLRVPGDTEWQSTYQSIGYPVLSVSTRT
                     DEGMSELRETLEGKISAFVGPSGVGKSSLINRILPGLDLGIAKVSEHHGKGRHTTRVA
                     TLHALPGGGYLADTPGIRELGTWALPIEQLDRCFPELGSFRDRCAFRSCSHTHEPNCA
                     VIDAVNEGTVNRERYESYVRLREEEIG"
     gene            complement(5447..>5826)
                     /locus_tag="VFG22_19225"
     CDS             complement(5447..>5826)
                     /locus_tag="VFG22_19225"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET6418438.1"
                     /translation="TPVALTGLMRGDYRVRVESPGFASWSTKVEVSQGETAGVTARLL
                     GKQGGAQPGAFGRVSIETTPPADVYVAGDLIGRTPIETVYAPLGRVTLDFELPDGRKV
                     SREVNVKSDGVARARFDLGNAKR"
BASE COUNT         1213 a         1798 c         1776 g         1039 t
ORIGIN      
        1 cgccacccag acgctccgct cggcggcaag caccgcctcc atgacttcgt cgtagtgctc
       61 gcggtcggaa aggatacgga gagagaccga gcggcgcatt cgaagaagat tacgcgaagc
      121 caacggcgag tcggagaaac cggcagaacc cgggcactgg cgctgggaga gtgacgcttc
      181 aagctgtacc gatcggaggg ctgctcacga cgcacgtgct cgcgattggg acccgctcgg
      241 ccgtctcctc aagtttccct acgtctagcc tcccgcccaa tcgctgtgcc gcgcatcccg
      301 agccgccctc ctatgcagat cccgacggat gcacactggc ggtgacactg gcactggcat
      361 ggaagtctcc gtacggaggg gtgcggggga gcgcgatgcc gggttccggc gacgtcgcga
      421 gtgacaatcg gccaggaagc aaccaccccc gattggcgtg agcgccgagg cgaggcttgc
      481 gtggtcaaac cccgccagtc cttccgacca caattgcaag ccgagccgtt cgacggaacc
      541 ccgcatcgcg ttcccccgcg ccgcgatggt cagtccattt cccggtactg ctcctcttcc
      601 gggttctcgt ctgcatccag aaacgccaga tctctctggt tgaatatgcg ctctagccca
      661 taccgaaacg gcacgaggag cgcgatgaac agaggaaaca gaactccaaa agacgactct
      721 ttgacgatcc agagcactga aaggcaagcg acttggatga gcgtgaatcc gtggaggacg
      781 gtccgtgaaa cgcggcggat gtagtgcgtt ggcggataga gcgtggggtc ggtgatccac
      841 agcttgaggc ggtcgacgaa ctggttgctt cggagcgatg cgattcccat gaagaggaac
      901 aagccaaaga cgaccgacat cggaatctct cgaagcacgc ctaaaaatac cagtgacccg
      961 ccaatcaaca ggtgaaccat caatggagtg aaccggttct ccctcacgga gatgatgtgc
     1021 tcccctcgtg catggtgccg gactgtcgcc aagcttcgga cgtgattcaa cgaccgaacg
     1081 gtcgccgcga ccacccaggg gaggccgatt gtcgaacatg cggcgaccag cacggcgatc
     1141 acggccatgt cgaggttgta tccctcgccc ttcttgagtc gatgctgtgg actgttgacg
     1201 agacgcacgg tgatgttctg atccaagaag agcaggaccg tggcgagaac cgcaatcgga
     1261 atcgatgcga tccagaacca gctcggggca tccatcgggt ttaccaacca cgctcgtccg
     1321 ctcgtcgtcg cgaactcgtg aggaacggcg agatgctcga gctggatcgg ccgcatcgcc
     1381 caggccgcag ccgtcatcgt gaagatcgcg atagctggtc cgaaatccgc caggaactcg
     1441 cggactgtct tcgtgagaag cggcttctgt ctcactcgcg aaagctgctg cgcgatcatg
     1501 aacgtcccga gtgcgaggat gagcgcgagc agctcgccgt tcgtcggcac ctcggcgtcg
     1561 aaaatgtccg cgaaaatgtc gcgcaatgcc tcggtaatga agatcagcgc gatcagcgcg
     1621 gcgaagatct cgtcggtgaa gcgcgtgaag tatcgaatgt accggctggc gttcgtgaga
     1681 gccaggaaca tcatgaatgc cgcagtccag atgccgatcc agaacaggcc aggaagaaac
     1741 gggactccga gttgcccaca aagcacgtac aagagagcta ggaagatcgt gaccggaccc
     1801 gtgcttccga gaagtgtgag gggctgcccc gaaaaaagtg cgtacgtcac acctccaatg
     1861 gcagtcgcca acaaggcttc gaccaccccg atctctccat gagtcagaaa ggcgagcagg
     1921 cccccgaaag cgatcgatgg cgccaaacaa gcgaagtaca ggaacaagat cgctgcgagg
     1981 cctttgacgt tgaatccatc gacgaaatcg cctcggtaaa acggtagctt tcgcctaacg
     2041 tccgcgatca gtcccgaggc aagacccgtt tgaggcggag gaacgctcgg atacgttggg
     2101 agaagcgacg gccggcgttg atccggtggg gcctcgacgt actccagata gagctggtac
     2161 gtcgccaaca cttgcatcgg gtcattcgca ccgatgatgg aggcgctgaa tcgctcgtca
     2221 tgcaacatcc atccaaacgg ctcgagatct tcgttggaag gcgtcgagcc gccgtaaggc
     2281 gcgacgatca gccaaacgta gtgaaccctc tcgtgatcgc tcgctcgaac accctcgggc
     2341 acacgaatca atgcgcagcg gcatgcatct ccgttctgcg cttcgtagcg cacgcgaaga
     2401 acgcctacgc cgctgccaag gtcgtgaagc tcgccctcac cccgtgcgcc cagagtgtct
     2461 tgaattgcgg cgcgctgccg gatgctcaac ctgcccaagt cgacgagcgc atccaaagcc
     2521 tgctgaacca aaccctgcag atcgcgggca gccaggtcga gtaagatctc gctgtcgtga
     2581 aggcgcttct gcaagctcat cccggtcgaa gtaaacgagg ttcaccgatc tccgttcgat
     2641 tgtgaaggcc gaacttggcg tggggagcaa aaaggggcga agactaggcc ccgatggagc
     2701 gacgaacgac ctgcatcaaa gtgtgcgtcc cccacttcaa cccatctacc ggaatgcgct
     2761 cgttgtgccc gtgaaacatg tccgcgaaac gcagcgaagg tgggagcttc acgggtgcaa
     2821 atccgtacca tttcgccccg atcgaagtaa acgccttcgc gtcggtgaat ccctgcatga
     2881 tgaagggcac gatgggcgag ctcggggccc gctccgacat gacctcgtga atcagcgaat
     2941 acaacgatga ctctttgggc tcggtgacca cgggcggcag cgaatgcatg atttcgagcg
     3001 caatgcccgt gcccaagacg tcgccgagct ctcgcatgaa atcgtctctg ctctgacccg
     3061 ggagaatgcg tccgtcgatc tccgcttcgg ccaagccagg aatgacgttg gtcttgtttc
     3121 cggctcgaag cacggtgggt gacgcggtgt tgccgagcaa agcgcgaaac gcgcgcctga
     3181 ccgaaggatc aggcaagatg ttgagcatgg caggtccgaa cgtcgggctt gccaacaact
     3241 tgatcgcagg acggatcggc gcgggaagat gcgatgcgag cgcggcaatg aagccctgaa
     3301 ccggcgcgga cgagtggcgc ggcaagccct ccttgcccag tcgcgcaatg gcctcactca
     3361 gttgaatgac agccgagtcc tctcgcggca tcgagccgtg ccctggctcc ccgattgcac
     3421 gcgcccggac ccagcacacc cccttctcgg caacctgaac cgtgaagaag ctccttccgg
     3481 cgacttgaat gttgaagcca ccgccctccc cgatggcaaa ctcgcttcgc accaggtcgg
     3541 catggttctc gcaaagccag agggaaccgt atctgcagcc ggcttcctca tcagcgaccc
     3601 cggcaaagat gagatcgcgt ttgggcttca cgccttcgcg ggccagccgt gtgaggatgg
     3661 cgaccgacat cgccgccatg ttcttcatgt cgacggcccc gcgcccccat aggcagtcat
     3721 catggacctc gccacaaaag ggcgggtggt cccaggccga tggatctgcc tcgaccacgt
     3781 ccaggtgagc ggttaggagc agcggcggct tttctcccgt gccgcgaagg cgcgcaacca
     3841 cgttgccgcg acccggagcg ctctgcatga cgagtggctc gaggccggca ccctctagct
     3901 cttcggcgag tagctcggct gcggggagct cgttgcccgg cgggttggtc gtatcgatgc
     3961 gaagcagctg ttggcaaaga cgaaatgcat gctccgcgag ctccggatca tcttcggtca
     4021 tgggagtgat cgaggtttac tccaagcggc ctcctttgtg cgcgatttct tcaacagcgc
     4081 ccatttttcg ccacgaggcc agtgttgctg ccccgacgct gcttgatcgg agatcgacat
     4141 tggctcaggg tcacggacta gatgagtttg tttgaacagc tcggcggcga gccggcgttg
     4201 cgggccatca tcgacgattt cgtcgatcgc tgtttcgacg acatgatgat cggcttcttg
     4261 ttcgcacgcg ccgagcggga acgcatcaag cgcttcgagt atgagcatgc tgcacagcat
     4321 ctcggcgctc ccgtggaata ccacgggcga cctctcgacg aggcgcacgg ccctcaccgt
     4381 atcttcggtg ggcagttcga tcggcggcgc cagatcctga tcgaaacgct caaggaccac
     4441 gatgcgtcgg ccgaggtcgt cgacgcgtgg atttctcatc aagacacgct ccggacgctg
     4501 atcacgcgcg acccggattc gaattgtcag ggctgagcat ggtcgagacg atagaaggcc
     4561 tcgtcgtgcg ttccacagca gggttcatcg atgtgcaggt cgacggcgaa gtgctccaag
     4621 cccgcatacg cggacggctc aagaacacgg cgcgccgcac cactgacctc tgcgtcatcg
     4681 gcgatcgcgt gcgcgtgtcg tgccccgacg agcgtagcta cgtcgtgagc gaggtgatgc
     4741 ctcgaaagac ccgcttttcg aggcgccagc caggacgggg ccctaacaag gaggacgttc
     4801 tcgtcgcaaa cctcgaccaa ctcttgatca ccttctgcta cggcccgccg gggctcaaga
     4861 cacgccttct cgatcgcttt ttggtgatcg ccgagcacca agatgttcat ccggtaattc
     4921 tcatgaacaa gtcggacctc cgcgtccctg gggacaccga gtggcagtcc acgtaccaat
     4981 cgatcgggta cccggtactg agtgtgagca cgcgcaccga tgaaggaatg agcgagctcc
     5041 gcgaaacctt ggaagggaaa atctcagcgt tcgtgggccc ctctggggtc ggaaagagca
     5101 gcctcatcaa tcgaatcctg cctggactcg atctcggaat agcaaaggtc agtgagcatc
     5161 acggcaaggg ccgtcacacg acccgcgtcg ccaccctgca cgcgctacct ggaggcggct
     5221 atctcgcgga tacgcccggg attcgtgagc tggggacctg ggcactgccc atcgagcagc
     5281 tcgaccgctg ctttccggag ctcggctctt ttcgggaccg gtgcgcgttt cgaagctgca
     5341 gtcataccca cgagccgaac tgcgccgtca tcgatgcggt gaacgaaggc accgtcaatc
     5401 gcgagcgata cgagagctac gttcggcttc gtgaggagga aatcggctaa cgcttggcgt
     5461 tgccgagatc gaaacgcgcg cgcgcaacac catcggactt gacgttgacc tcacgactga
     5521 ccttccggcc gtcgggcagc tcgaagtcca aggtcacgcg acctagcggt gcgtagaccg
     5581 tctcgatcgg cgtccgtccg atcaaatcgc ctgcgacgta cacatcggct ggcggggtgg
     5641 tctcgatgct cacgcgacca aacgcccccg gctgtgcgcc gccttgcttg cccagcagtc
     5701 gcgctgtcac gccggcggtt tctccttgtg acacctctac cttggtcgac caggacgcaa
     5761 agccgggtga ttcgacgcgg actcgatagt cgccgcgcat gagcccggtc aatgcaacgg
     5821 gtgtta
//
LOCUS       DASRZF010000439        23832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4466171, whole genome shotgun sequence.
ACCESSION   DASRZF010000439 DASRZF010000000
VERSION     DASRZF010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 23832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23832
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4466171"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VFG22_19230"
     CDS             <1..553
                     /locus_tag="VFG22_19230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418439.1"
                     /translation="TDFGFATLMPTASAVNVNQVPADDVDAMVANGWSYVIEGTITRV
                     SDGATKEFLIQADVPSTYSACAVDGLEPGVNVSRNSSVDITLHGDHIFFNGFPEEEGN
                     VVRLAQWMWDIEDTDGNDVLTQTDFQAATDVGALFPSLRLGVYELTGGPISPIDDAWD
                     FIRAQLGTQGHIFGEGECEWTPL"
     gene            608..2074
                     /locus_tag="VFG22_19235"
     CDS             608..2074
                     /locus_tag="VFG22_19235"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET6418440.1"
                     /translation="MQEGNDFPIAIIGAGFAGIGAAIQLIKSGIHSFTIFERASEIGG
                     TWRDNTYPGAACDVPSHVYSLSFEQNPNWSRRYATSWEIQDYLLGVVAKWKLRERMRL
                     NTEIVGARFDEGSGTWSLETDRGNTFRARVVISGVGGLVDPSYPNIPGMETFEGEMIH
                     TARWKHEVDLSNKRVAVIGTGASAVQVVPSIAPLVAMLSVFQRTPAWVVAKFDHLYPR
                     WLRKFLARVPFFLHASRFVKYWLSELRGPIVFYDSKLLSKIGVLLSKWNLRRQVKNPE
                     LRKKLTPDFQFGCKRVLVSDDYWATFERENVDLITDAIDKIESKGVRTKDGTLYEADA
                     IVLATGFKLGLATAPFPVVGRGGRTLDEAWKNGAVAYKGMTVAGFPNWFIMMGPNTGP
                     GHTSVLVYTEAQISHAVQAIQKLMREGLKYIDVRQDVQDRYNAGIQERMKRMVWGGCK
                     SWYLNEDGSNHSLYPGFAAEYVLRARNLDLSDYEFAGF"
     gene            complement(2117..2719)
                     /locus_tag="VFG22_19240"
     CDS             complement(2117..2719)
                     /locus_tag="VFG22_19240"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6418441.1"
                     /translation="MESERPYHHGSLRQTLLETAFELVDKEGSGAISMRALARAAGVS
                     SAAPFRHFSDRSVLLRAVAEKAAAELDSKLDEASAECGDALTQFRAMTVAYVRYAAEH
                     PALFDLIQSTAPFFGGLLGGVNDERRLKLIALIYEGQNAGLIPEADPELIALSSEALT
                     HGLARMIVDQHPRVRNLSSEEARKLALAATQLLQSGIGLA"
     gene            3199..3624
                     /locus_tag="VFG22_19245"
     CDS             3199..3624
                     /locus_tag="VFG22_19245"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET6418442.1"
                     /translation="MAAKHILACIDFSKQSTLALNEVGEYARANGSKVTLIHISDPRA
                     FIPPQAVLQPATATNSKEQEAALEKLRDQHLKDLDVEIAVRSDHAPARAICDYAEEHD
                     ADLIVLGSHGRGGMERWLIGSVAERVVRHASSNVYIVRR"
     gene            3661..4530
                     /locus_tag="VFG22_19250"
     CDS             3661..4530
                     /locus_tag="VFG22_19250"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6418443.1"
                     /translation="MKKRRAWSILGNSQRLDGGAMFGNAPRALWQKWIEPDELNRIPL
                     ACRALLVEDEGRLILFEAGIGAFFPPQLRDRFGVQEDRHVLLDNLEKAGFAHEDVDCV
                     VLSHLHFDHAGGLLSAYDPAEPMRLLFPKATFVVSWDAWNRAKHPHARDRASFIAELP
                     PLLEESGRLEVVKGHRSETLGSDYKLWLSEGHTPGLMLTEIPGADGPLVYAADLIPGR
                     AWVHLPITMGYDRYPEQLIDEKTDLLARLVEQRGRLFFTHDHEVAIARVSRNEKGRFS
                     PEEPRAELTGAPL"
     gene            4565..6046
                     /locus_tag="VFG22_19255"
     CDS             4565..6046
                     /locus_tag="VFG22_19255"
                     /inference="COORDINATES: protein motif:HMM:NF023222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HET6418444.1"
                     /translation="MPLGNGDTPPRIETGRDYIESLRGRNLKVFLFGELVDEPVDHPM
                     IRPSINAVAETYDLAIREPEVATVESPLTGKRVNRFLHVALSAEDLVAQNMMQRKLGQ
                     NTGTCFQRCVGMDAFNSLYSVTYEIDEKHGTPYHARLKGFLETMQERNFVVGGAMTDV
                     KGDRSKGPSEQEDPDLFVHVTKRSAEGIWLKGAKAHQTGCINSHWIIAMPTMRLRPDD
                     KDYAVVAAFPVDAQGITYVYGRQSCDTRSMEGGSIDVGNARYSGQEAMVILDDVFVPW
                     SHVFMDGEHEFASMLVERFTCYHRRSYVCKTGVGDVLIGAAAQAAEYNGVDRASHIKD
                     KLVQMTHLNETMYAAGIASSHQARPTRSGCYLNDDMLANVCKHHVTKMPFEIGRLAQD
                     LAGGLVSTAPSEKELKHPELGRLLDKYLRGRADVPTESRIRVLRLIENMTMGRNAVGY
                     LTESLHGAGSPEAQRIQIGRQMNLDFKKGLARRLSGIDSDGED"
     gene            6091..7215
                     /locus_tag="VFG22_19260"
     CDS             6091..7215
                     /locus_tag="VFG22_19260"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HET6418445.1"
                     /translation="MRTRRWSWAIVAYGLVAIAGCDRGATAETPVQGIEVGATRTGGS
                     EREGANAERGADQEGLLIGEYELPDDAIVDGDTIRVRGIEDSIRLLSIDTEEKLRSRA
                     DRTAAKEDFEQYLRNKRGNATRPQKAGTPMGEKATEFAEAFFAGAEVVRLERDDPKDI
                     RGRFGRLLAYAFVEKNGRWTSYNVECVRAGMSPYFTKYGYSHRFHNQLSHAEAEAREA
                     GRGIWDPRSKGYGDYEERKAWWNARADFIRQFEHEANRRNDHIQLAQLDALEKLEENL
                     GREVTVLSTVDRIRHFKGLVRVSLALQSEGAFPLIFFDRDVYRRSGIERYKGEPVSVR
                     GKVERYEKGDYRTLQIVVHEPAQVTLPSLPWTENAQSVPD"
     gene            7291..8913
                     /locus_tag="VFG22_19265"
     CDS             7291..8913
                     /locus_tag="VFG22_19265"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HET6418446.1"
                     /translation="MSGDRTCTRAFACLLWLLGACGRETLLIEPRCDGLAPGDLVITE
                     VHANPDGSDGEAEYIELFNASPEQLSLDGLVLATGRADGTGVKTHRFQGVSISEGDFF
                     VVGNAAPDSMPAHLDYSYGTALGSLRNSDAFVSVECGETLIDEMRYEGTNDGRALELD
                     GGLAPNHELNDDTNLWCPTPEGTDEFVPGNIGTPGARNSSCAPIELEGNCLDDNVVRA
                     SRSPGPGQVQITEWMANPAGPDADFEWVEVLFGEAVDLHGFQLGPSPDSLEAVIDGED
                     CFPVDAGRRVVFGASPAATPRVDADLGFSLGNSGARSVVAGANGRVVDRVDYDGTVEG
                     VAWQIDPVGELCLASSDEEYATGNLGTPGEANTFCPQVLDPGTCLEDGVPRKIVSPKA
                     GEAWISEWMANPSSVGSREGEWLELRFDAEVDLNGLVLTDLTSSVTRIDQKDCIRVSA
                     GSHRVFARSSDSSENGGITGVLGELSFSLNNSDETITLSVEGQTLDTVSYERSTLGVA
                     TQVDELGKRCDAMHRYGDGDLGTPGLPNPWCS"
     gene            8904..10973
                     /locus_tag="VFG22_19270"
     CDS             8904..10973
                     /locus_tag="VFG22_19270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418447.1"
                     /translation="MLLRSSLVFACVLALAARSARAAQYEVFIDVQTEEDLYDLLVTG
                     QISKSSFEALLLLYQTRVDLNRAGRPQLYLLPNLDYDQVDRILAYRREAGSIHQLEDL
                     VESGALEPELVRALGSFVIVRPADAPRSRVDGVLRIQALWSGRNDRLPPAMALQARVK
                     AARNLDAGMAATLTRNGLGRVRWDRTRGALSAQPERARITAPKLYVAWSDARWEVIAG
                     TYRIGFGQRLTFDVTDQITPNGFIGDYGLRRGGELELRCKRAAGELYESPCSRERVQR
                     VTPDFDWSNRLSGIALGLRDQAIGKGWLQAYAWGSYQVHRAPQIELADRNLCDDPARD
                     ADPACRAPVVYVRGADPSAPSSTATFASLPVMYAEGLAGMNLTYFWSDRAQLGLTGYG
                     SVPRWLIDGAELGFQEFARKPFVGAFGALGLNGSFGFGAQDFFVELARSFDALRGSGG
                     LGAIARSVSTLPGAEIEVSARYYEPSYANPYARPISAPDELDGLRARDEAGLRIRVTA
                     VRGRRFGLRTAADGWRRLTSGAFGSVLFARADLQLASAWTLALWAEHRNTGVQRFLLA
                     TKLAYTRGRRVSVSAQLEHAWSQIRLAAGGAQQDIAAVLSLTTQPHELLRLRLRVRYD
                     FEDIWNNHRLPQAVWGYLNAALTLRERGLLRIRYDLRAFLDERGSTSARAPNPEHWIW
                     LEYAFRY"
     gene            10997..12586
                     /locus_tag="VFG22_19275"
     CDS             10997..12586
                     /locus_tag="VFG22_19275"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET6418448.1"
                     /translation="MGTFGNQIEAARVLYRAGVFKPIRPDKTLRIIKAAKGWGRSPAM
                     GFIALAIRQPDTVAVIDDEGEATFDEINRRSNALARGIQEAGISPGDVVGVMCRNHRG
                     FVETLIACCKVGANAVLLNTMFAAPQLAEVGRRESIRALFYDEEFGTLIESFRGMPRF
                     VVYGAGVDPAIGEVIANHADHDPEPPSIESRFTMLTSGTTGTPKGAKRGSPKGILPIA
                     SVLSRLPLRAGERTMIAAPLFHSWGLGQFQLGLLLGTTYVLNRRFDPEATLRAVSEHR
                     CTSLAVVPIMMQRILALPDDIKRKYDLSSLRVTAASGSALPGHLATEWMDTFGDNLYN
                     FYGSTEVAMASIATPEDMRAAPGTVGKPPLGTSVAILDGQGQRLPNGETGRIFVANAI
                     TFEGYTSGDDKERLGGMVSSGDLGHFDDDGRLFVDGRDDDMIISGGENVFPREVEDLI
                     ASHRSVAETAVIGVSDGEWGQRLKAFVVLRSGADLSEDGVRVYVKQRLAGYKVPRDVV
                     FIDALPRNATGKVLKRELEQH"
     gene            complement(12583..13515)
                     /locus_tag="VFG22_19280"
     CDS             complement(12583..13515)
                     /locus_tag="VFG22_19280"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6418449.1"
                     /translation="MAGPFSNLDGTSNAAGLSRILKWQLGFHDEKRPRTPATGIPVPA
                     VSNDGKALRHAVRDALTWVGHATFLVQLGGRSALIDPVMSQRLSGVVQRNVAPGLAWS
                     ALPKIDVVFITHNHRDHMDVPTLKRLGPDPVYVVPRGLGTSFERAGFRRVVEMDWWQE
                     EEIEGLRVTFVPSQHWSRRGLTDMNTTWWGGFVIERGGLRVYHSGDTAWFEGFSLIGA
                     RCGEIHASMLPIGAYAPRWFMRSQHMDPGDAVRAFSALGSEKFVAMHWGTFKLTDEHL
                     GEPPELLRDVWERAGLHDDQRFIPAIGETLPLDR"
     gene            complement(13536..14294)
                     /locus_tag="VFG22_19285"
     CDS             complement(13536..14294)
                     /locus_tag="VFG22_19285"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET6418450.1"
                     /translation="MNEIANAPDIRGEAVSYQAGGTRLEGYLAWDASKTGPRPGIIVV
                     HEWWGHNEYVRRRARMLAELGYAAFALDMYGDGKNTQRPEEAQKLLMAAVGDPAVAEQ
                     RFFAACEVLKKHPATDPSKIAAIGYCFGGAVVLQMARRGADLAGVASFHGTLSPQGPP
                     AQPGAVKAKVLVLHGAADPLVPAEQLAAFEKEMEAAGVDCSVIEYPGATHAFTNPEAT
                     ERGQKLKMPLAYDKSADEQSWAELRKFLQGLFAG"
     gene            complement(14344..16434)
                     /gene="fusA"
                     /locus_tag="VFG22_19290"
     CDS             complement(14344..16434)
                     /gene="fusA"
                     /locus_tag="VFG22_19290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET6418451.1"
                     /translation="MKDLSKYRNIGIFAHVDAGKTTTTERILKLTGKIHKIGEVHDGA
                     ATTDFMEQEQERGITIQSAATTCFWNGHQLNIIDTPGHVDFTIEVYRSLKVLDGGVGV
                     FCGSGGVEPQSETNWRYANDSKVARLIFVNKLDRLGADFYRVVGQIKSVLGAKPLVMV
                     LPIGTEGDFSGVIDLLTRKAYVWDDSGQPENYEVKEVPEDMKELVDKYRFELIEAAVE
                     QDDDAMEQYLEGTEPDLETIKKCIRIGTRDLVFFPTYCGSAFKNKGIQLVLNAVVDYL
                     PDPTQVPPQPEIDLEGNETGKFAIVDPDGPVRALAFKIMEDRFGALTFTRIYSGTINK
                     GDTLINTFTGKSERIGRMVEMHADDRTIIDSAQAGDIVAMVGLKDVRTGHTLADQKNP
                     ATLEPMVFPDPVISIAVSTKDKANSEKLGTALGKMVAEDPSFHVEVDQESGETILKGM
                     GELHLDIKVDILRRSHGVAVDVGAPQVAYRETVTQAIQDSYTHKKQTGGSGQYAKIDY
                     TIEPAEPGEGFVFESKITGGNIPKEFIPAVEKGFKVMKDEGPLCGFPLLDFKVSLTDG
                     SYHAVDSSQMAFETAARAAFRQSMPKAGPQLLEPIMKVDVYVPDANVGDVIGDLNRRR
                     GIVNSQEKGHTNVRIKADVPLSEMFGYIGDLRSATSGRGQFSMEFSNYSPVPRNILEK
                     VQIEVAERKAKKKK"
     gene            complement(16644..16865)
                     /locus_tag="VFG22_19295"
     CDS             complement(16644..16865)
                     /locus_tag="VFG22_19295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418452.1"
                     /translation="MAMFIRVDVDHKVVEKTPGLAAKLVEVCPVKIFKLGSKSNAVEV
                     VEENVDECTLCDLCMQASPEGVKVIKLYE"
     gene            complement(16883..17782)
                     /locus_tag="VFG22_19300"
     CDS             complement(16883..17782)
                     /locus_tag="VFG22_19300"
                     /inference="COORDINATES: protein motif:HMM:NF012758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HET6418453.1"
                     /translation="MAVIVHEDFQFIVQGITGREALNFTRECLQYGSKIIGGVTPGKG
                     GREVYGVPVYDTVREITEKERVDGTVITVPLAFAPDAAYEAIDAGIKEIIIITEGIPR
                     RDASAIVEYAELHGARLIGPNCLGLIVPDVCRFGSLGGPAVDCRKAFKPGVVGVMSRS
                     GGMTTEICNALTLSGLGVSTAISIGGDPVIGSTYADLIPLFEADEDTKAVVVYSEPGG
                     TAEAELARWTQEHKSRLPIVAFVAGRFMDEMPGMSFGHAGTVVNKKVDSPADKMRRMR
                     EAGISVAEEIGDIPNLVRERIGN"
     gene            complement(17794..18987)
                     /locus_tag="VFG22_19305"
     CDS             complement(17794..18987)
                     /locus_tag="VFG22_19305"
                     /inference="COORDINATES: protein motif:HMM:NF020031.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET6418454.1"
                     /translation="MRFYEFEAKQLLKKHRIPLVRGELAKTADEAKKIASDIGGPVVV
                     KAQAIAPGLAAASVRDAADPAAAKAVAAELLALDDGGRKPNGVLVEAKPVAEAVYSLS
                     FTYDGSSKRPVVAAAAMAGKIDDLGDTHPDRIVRRQFSALIPFSDYIAKELAKSLGLG
                     GSDLNRMTRIVSGLARLFLEYDLADLDITSMAKTADGNFVVCDVEADLEIEARHRQRP
                     MLDELGFSKDDLRQVREPTEFELEGARIDAEDPRGIISPIAEFDGNIGLVIGAGGGSL
                     TLTDAVRKHGGRPANYAAIGGNPSVDKARRLTKLVLSKPGVEKIAVMSNVVSNTRADL
                     VARGVIKGIKELGFEPADKILIFRVPGAWEADAAKILQKYGVEYCDRTVSISEAARRA
                     VEKVG"
     gene            complement(19026..19892)
                     /locus_tag="VFG22_19310"
     CDS             complement(19026..19892)
                     /locus_tag="VFG22_19310"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HET6418455.1"
                     /translation="MSKKHRPRRSVLYMPGANARALEKARSLPADSLILDLEDAVAPD
                     MKETARSQVVSAVKAGGYGRREVMIRTNGLNTPWGYDDLVAAAAVRPDAVLLPKVESA
                     ETVRQAESVLVSHGASPDLAIWCMMETPRAMLHAEEIADASPRLGGLVMGTSDLAKDL
                     QAQHTAMRLPLITALGLCLLAARAARLTILDGVYLDLNDHEGFVDSCRQGAELGFDGK
                     TLIHPKQLEAANEIFAPSEEELRLSRRIIEAYAEAEAQGKGVVVVDGKLIERLHVDHA
                     ERVVALAEAIAP"
     gene            complement(19904..20953)
                     /locus_tag="VFG22_19315"
     CDS             complement(19904..20953)
                     /locus_tag="VFG22_19315"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HET6418456.1"
                     /translation="MRNPKDFFKPMAIGAPEPPREIPFRPSRMIHFFDPSNPKMAAKA
                     PSIAAKCDVLLGNLEDAISADKKTEAREGFVKIAKENEFGDCQLWTRVNSLDSPWFLD
                     DMWQIVQEVGDKVDVIMIPKVEGPWDIHYVDRLLAQLEARAGIKRPILIHAILETALG
                     VANVEEISGASPRMQGLSLGPADLAASRRMKTTRVGGGHPGYLVRQDPDVDDPEAPRA
                     TAQQDLWHYTIARMVDACVANGIFAYYGPFGDIRDVVACEDQFRNAFLLGCVGAWSLH
                     PVQIDIAKKVFSPPVDEVLWAKKVIREMGDGTGAVMIDGKMQDDATVKQCKVMVELAR
                     MLAEKDADLKAAYQF"
     gene            21022..21894
                     /locus_tag="VFG22_19320"
     CDS             21022..21894
                     /locus_tag="VFG22_19320"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET6418457.1"
                     /translation="MEIGMNLPVMAPGLDRDTFLAWCERIDAGFFASLAAGERISFYN
                     PDMTAALAAASVLTKRVKIVSGVFVPMLHHPVMLAKQLATIDVLSGGRFVAGLGVGGR
                     EQDYQSVDAPLGKRLTRLAESVKTMRSVWRGEPVVEGALPVGPAPVQDGGPKLLAGSL
                     MVESIQTASRWADGLCGFSFGPSPAEMELQFGAARRAWKDRGRPAPWLGTGFWYALGP
                     NARAQLDAYLDRYLNFMAPVARESVKQICIATTPQALKNAIQQAKDAGADDVLLVPTT
                     SDPDDVHRVTDILG"
     gene            21953..22219
                     /locus_tag="VFG22_19325"
     CDS             21953..22219
                     /locus_tag="VFG22_19325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418458.1"
                     /translation="MRAIIVAMFLVGAFVAGLLIAEANPAAADGPPKKAQWQYQCFEA
                     DGVAQVTERANGMGEQGWELVTSAGTVKGPTLWCFKRPLWKPKK"
     gene            22294..23142
                     /locus_tag="VFG22_19330"
     CDS             22294..23142
                     /locus_tag="VFG22_19330"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET6418459.1"
                     /translation="MSFLDDPQVRRNVERLPIEFDDYGFDRFGLSRKALIRAYSPMAY
                     IYRRYLKVTAFGLENVPAKGRGLVIANHSGGLGADAAMIFTSLVLNDEAPRLGQGMAE
                     YFLTRSPFAAPALRKLGHLTGLPEHGEQLLNDERLVVVFPEGARGAGKLYKDRYKLVR
                     FGTGFMRLALKTGAPIIPCAFIGGEESFPQLFHIKWLAKLVNGPFVPVAPQLIFFPLP
                     VACQVYYGPPMHFEGDGSEPDHVIQQYIEEVRHSMERLISGGLDARPRAFMFERMPDA
                     EKERRP"
     gene            23139..>23832
                     /locus_tag="VFG22_19335"
     CDS             23139..>23832
                     /locus_tag="VFG22_19335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET6418460.1"
                     /translation="MKILITGISGTLARGVAYRLVRRGYQVVGIDRRPWPDVPEGVEM
                     HQADIRKRPAEDVFRISRPDALIHMATVTHFTTDTEERYRINLGGTRKLFEYCHEYGV
                     KQAVFVGRHTVYGATPDSPLYHTEDEPPLAGAAFKELADLVAADLYAGSALWRWPELN
                     TAVLRLCYFLGPSRGGTLASFLSKNRVPTVLGFDPLYQLMHEWDAEEAITLAVENQLR
                     GVFNVAGPPPVPL"
BASE COUNT         4500 a         7337 c         7445 g         4550 t
ORIGIN      
        1 caccgatttc ggtttcgcga cccttatgcc gaccgccagc gcagtgaacg tcaaccaggt
       61 accggcggac gacgtcgatg ccatggtcgc gaacggatgg agctacgtca tcgaaggtac
      121 gatcacgcgt gtttccgacg gagcgacgaa ggaatttctc attcaggcgg acgtgccctc
      181 gacctacagc gcgtgcgccg tggacggcct ggagccagga gtcaacgtca gccgcaactc
      241 gagcgtcgac atcacgctgc acggcgacca tatcttcttc aacggcttcc ccgaagaaga
      301 aggcaacgtc gtccgtcttg cgcagtggat gtgggacatc gaggacaccg acggcaacga
      361 tgtactgacc caaacggatt tccaagccgc aaccgatgtg ggcgccctat tcccatcgct
      421 gcggctaggc gtgtacgagc tcaccggcgg cccgatctcg ccgattgacg atgcctggga
      481 tttcatccgc gcccagcttg gtacccaggg ccacatcttc ggcgagggcg aatgcgaatg
      541 gacacccctc tgaggggttc gggcggggac tggcactggc gctggcgctg ctctaacttg
      601 cgcgaccgtg caagaaggca atgactttcc gatcgcgatc atcggggcag gcttcgctgg
      661 gattggcgcg gcgattcaac tgatcaagtc gggcatccat tccttcacga tcttcgagcg
      721 tgcgagcgag atcggcggga cctggcggga caacacctat ccaggcgctg cctgcgacgt
      781 accttcgcat gtttattccc tttcgttcga gcagaacccc aactggagcc gtcgctacgc
      841 aacatcctgg gagatccagg attaccttct cggcgtcgtg gccaagtgga agctgcgcga
      901 gcgaatgcga ctcaacaccg agatcgtcgg agcgcgtttc gacgagggct cgggcacctg
      961 gtctctcgag acggaccgcg ggaatacttt cagggctcgc gtggtcattt caggagtggg
     1021 cggcctggtc gatccttcct atccaaacat cccaggaatg gaaacgttcg agggtgaaat
     1081 gatccacacg gcgcgctgga aacacgaggt cgacctcagc aacaagcgcg tcgcggtgat
     1141 cggcacgggc gcgagcgccg tccaggtcgt accgagtatc gcgcccctcg tggcgatgct
     1201 gagcgtcttt caacgcactc ctgcctgggt ggtcgcgaag ttcgatcatc tgtacccgcg
     1261 ctggcttcgg aagtttctgg cgcgcgtgcc gttctttcta catgcgagtc gattcgtgaa
     1321 gtactggctg agtgagctca ggggaccgat cgtcttctac gactcgaagc tgttgtcgaa
     1381 gatcggggtt ttgctttcca aatggaatct caggcgccag gtgaagaacc cggagcttcg
     1441 gaagaagctg actcccgact ttcagtttgg ttgcaagcgg gtcctggttt ccgacgacta
     1501 ctgggcaacc ttcgaacgtg aaaacgtcga cttgatcacg gacgcgatcg ataagatcga
     1561 gagcaagggt gttcgcacca aggacggcac gctgtacgaa gcggacgcaa tcgtgctggc
     1621 gaccgggttc aagctcggcc tcgctacagc gccgtttccc gtcgttggcc ggggcggccg
     1681 cacgctcgac gaagcctgga agaacggcgc ggtcgcgtac aagggaatga ctgtcgccgg
     1741 cttccccaac tggttcatca tgatgggccc gaacaccggc cccggtcaca cctcggtcct
     1801 cgtctacacg gaggcgcaga tctcccacgc agtgcaagcg atccagaagc tcatgaggga
     1861 aggcttgaag tacatcgacg tccgtcagga cgtgcaggac cggtacaacg caggcatcca
     1921 ggagcggatg aaacgcatgg tctggggtgg ttgcaaaagc tggtacctca atgaggacgg
     1981 cagcaaccat tccctctacc caggcttcgc cgcggaatac gtgctccgcg ctcggaatct
     2041 cgacctcagc gactacgagt tcgctggctt ctgatcggaa ccaaaccagc cgtgatgccg
     2101 ccggagctac actcgctcaa gcaagcccga ttccgctttg gagcaactgg gttgcggcca
     2161 gagcgagctt gcgggcctcc tctgagctga ggttccgcac gcgcgggtgc tgatcgacga
     2221 tcatgcgggc caggccatgc gtgagggctt cactgctgag cgcgatgagc tccgggtcag
     2281 cctctggaat gaggcccgcg ttctgacctt catagatgag cgcgatcaac ttcagtctcc
     2341 gctcatcgtt gaccccgccc agaagacccc caaaaaaggg cgcagtgctt tggattaggt
     2401 cgaaaagcgc cgggtgctcc gcggcgtagc gcacgtatgc gactgtcatc gcgcggaact
     2461 gggtgagcgc atcaccgcat tccgcactcg cctcgtcgag cttgctgtcg agctcggcgg
     2521 cagccttctc tgcgactgcc cggagcaaca cgcttctgtc cgagaagtgt cgaaacggtg
     2581 ctgccgaaga aacgcccgcc gcgcgggcca gcgcacgcat gctgatcgct ccgcttcctt
     2641 ccttgtcgac gagctcgaaa gcggtctcga gcagcgtctg gcggaggctt ccgtggtggt
     2701 agggcctttc gctttccatc tgagccgaga ctagcaaagg atgttatcgg cgcttacttt
     2761 ctgcacgccc tgcatgtaag cagtgataac acctgaatct ctgcgatgtt agcgatgctc
     2821 acattgctca tcccaggatg ttatcaacgc ttacatccga tcggcgctca ttggcagact
     2881 ttctcggagt agtcgcattt gtagtcgtac gggcaatctt tgctttcgag gcaggggagc
     2941 cgctcacaaa gccctgtagc ctgattacag aagtaggggg ccgcgcagga atgcagtcgg
     3001 caggccacct cggaacgcga gagctttccc ggccgcggcg gggtttcctc gctgctgatc
     3061 ccggcagcgg tcgttccctc cggcttcacc gatctgttgc atccgagctg agcgaaggcc
     3121 aaacacgacg ccagcgcgaa cagatgccac caaggtcgac gcatgccgtt catctgacaa
     3181 agatgatagc tttcggccat ggccgcaaag catatcctcg catgcatcga cttctcgaag
     3241 cagtccacgc tcgcactgaa cgaggtgggc gaatacgcga gggcgaacgg tagcaaggtc
     3301 acgctcatcc acatctccga tccgcgagcg ttcattccgc cgcaggccgt attgcagcct
     3361 gcaacggcaa ccaactcgaa ggaacaggaa gccgccctcg agaagctccg cgaccagcat
     3421 ttgaaggacc tcgacgtcga gatcgccgtt cggagcgacc acgcgccggc cagagcgatt
     3481 tgcgactatg ccgaggaaca cgacgcagac ctgatcgtcc tcggatcgca cggccggggc
     3541 ggcatggagc gctggctcat aggaagcgtg gccgagcgcg tcgtgcgcca cgcctcttcg
     3601 aacgtgtaca tcgttcggcg gtgacttcaa aggatttggc taccctgcta agcttccccg
     3661 atgaagaaac gccgggcctg gtcgatcctg ggcaattcac agcggctcga cgggggagcc
     3721 atgtttggca acgcgccgcg cgcactgtgg caaaaatgga tcgagcccga cgagctcaac
     3781 cgtattccac tcgcttgccg ggccctactc gtcgaggacg aaggccggct gattctcttc
     3841 gaggctggta tcggtgcgtt ctttccccct cagttgcgcg accgcttcgg agttcaagag
     3901 gatcgacacg ttcttctcga caatctggag aaagcaggat tcgcacacga agacgtcgat
     3961 tgcgtggtcc tgtcgcattt gcacttcgat cacgccggcg gcctgctcag tgcctacgat
     4021 cctgcggagc cgatgcgttt gctcttcccg aaggcgacgt tcgtcgtgag ctgggacgcc
     4081 tggaatcgcg ccaagcaccc gcacgcccgc gaccgagcgt cgtttattgc tgagctcccc
     4141 ccattgctcg aagagagcgg acggctcgaa gtcgtgaagg gacatcgctc cgaaacgctc
     4201 ggttcagact acaagctttg gctctctgaa ggacacacgc caggactcat gctcaccgaa
     4261 attccgggcg ccgacgggcc tctcgtgtac gccgccgatt tgattcccgg ccgcgcctgg
     4321 gtgcaccttc cgatcaccat gggctacgac cgctacccag agcagctgat cgacgaaaag
     4381 acagatttgc tggcgcggct cgtcgagcaa cgaggtcggt tgttcttcac gcatgaccac
     4441 gaagtcgcca tcgcgcgggt cagccgcaac gagaagggac gcttttcgcc tgaagaaccg
     4501 cgggcggagc tcacgggcgc gcccctctaa tgggcgtggt aaagtctcag gcctaggaga
     4561 aacgatgcct cttggcaacg gcgacacgcc cccacgaatt gaaaccggtc gcgactacat
     4621 cgagagcctt cgcgggagga acctgaaagt gttcctcttc ggggagttgg tcgacgagcc
     4681 ggtggatcac ccgatgatcc ggccttcgat caacgcggtc gccgagacct acgacctggc
     4741 gattcgggaa cccgaggtgg ccaccgtcga atccccgctc accgggaagc gcgtgaaccg
     4801 ttttcttcat gttgccctca gcgccgagga cctggtggcg cagaacatga tgcagcgaaa
     4861 gctagggcag aacaccggca cctgctttca gcgttgcgtc gggatggatg cgttcaactc
     4921 gctctattcg gttacgtacg aaatcgacga gaaacacggc acgccctacc acgcacggct
     4981 caagggcttt ctcgagacga tgcaagagag aaacttcgtg gtcggcggcg cgatgacaga
     5041 cgtcaaaggc gaccgaagca aaggtccgag cgagcaggaa gatcctgatc tcttcgtgca
     5101 tgtcactaaa cgaagcgccg aaggcatttg gctcaagggc gcaaaagcgc accagaccgg
     5161 ctgcatcaat tcacactgga tcatcgcgat gcccacgatg cggctccgcc ccgacgacaa
     5221 agactacgcg gtggtcgcgg cctttccagt ggatgcgcaa ggcatcacgt acgtctatgg
     5281 gcgccagtcc tgcgatacgc ggagtatgga aggcggatcg atcgacgttg gcaacgcgag
     5341 gtactccggt caggaagcga tggtgatcct ggacgatgtg ttcgttcctt ggagtcacgt
     5401 gttcatggac ggcgagcacg agttcgcatc gatgctcgta gagcgcttca cctgctacca
     5461 tcgccgaagc tacgtctgca agaccggcgt aggcgacgtc ttgatcggcg ccgcagcgca
     5521 agcagcagaa tacaacggcg tcgaccgagc ctcgcacatc aaagacaagc tcgtgcagat
     5581 gacgcacctg aatgagacca tgtacgcagc agggatagcc tcctcgcacc aggcgcgccc
     5641 gacccgctcg ggctgttact tgaacgacga catgttggcg aacgtgtgca agcaccacgt
     5701 caccaagatg ccattcgaga tcggacgcct cgcgcaggac ttggccggcg gtctagtcag
     5761 cacggcaccg tcggagaaag agctgaaaca tccggagcta gggcggctac tcgacaagta
     5821 cctgaggggc cgggctgacg ttccgacgga gtctcgcatt cgcgttttgc gattgatcga
     5881 gaacatgacc atgggtcgga acgctgttgg ctatctgacg gaatcgctgc acggcgccgg
     5941 ctcgcctgag gcacaaagaa tccaaatcgg gaggcaaatg aacctcgact tcaaaaaagg
     6001 gctcgcccgg cggctctcgg gcatcgattc cgacggcgaa gactaagtgc ccactcgcgc
     6061 aagagcgcga aattgaatag tgtacgcggg atgcgaacgc gaaggtggag ctgggcgatc
     6121 gtcgcttacg ggctcgtcgc aattgctgga tgcgaccgcg gcgccactgc cgagacgcca
     6181 gtccagggga tcgaggtcgg cgcgacgcgc acgggcggaa gcgagcgcga aggagccaac
     6241 gcagagaggg gcgccgatca ggaaggcctg ctgatcggcg aatatgagct gcccgatgac
     6301 gcgatcgtcg acggcgacac cattcgcgtg cgcggcatcg aagactccat tcgactcctg
     6361 tcgatcgata ccgaggagaa gctgcgcagc agagccgatc gaacggccgc caaggaggac
     6421 ttcgagcagt accttcgaaa caagcgcggc aatgcaaccc gtccgcagaa ggctggaact
     6481 ccgatgggcg agaaggccac cgagtttgcc gaagcgttct tcgccggggc cgaggtcgtg
     6541 cgcttggagc gcgatgaccc aaaggacatt cgcgggcgct tcgggcgctt gctggcctat
     6601 gcattcgtcg agaagaacgg gcgctggacg agctacaacg tcgagtgcgt ccgcgccgga
     6661 atgagtcctt acttcacgaa gtatgggtac tcgcatcgct ttcacaatca gctttcgcac
     6721 gcggaagccg aggcgcgtga ggccggccgc ggaatctggg atccccgctc gaaggggtat
     6781 ggcgactacg aggagcggaa agcgtggtgg aacgcgcgcg cggacttcat tcggcaattc
     6841 gaacacgaag ccaatcgacg caatgaccac atccagctcg cgcagttgga cgctctcgag
     6901 aagctcgaag agaacctcgg ccgtgaagtg acggttcttt cgacggtcga tcggatcagg
     6961 catttcaaag gcctcgtccg agtgagcttg gcgcttcagt ctgaaggcgc attcccgctc
     7021 attttcttcg atagggacgt gtatcgtcgg agcggcatcg agcgctacaa gggcgagccc
     7081 gtcagcgtgc gaggcaaagt ggaacgctac gagaagggcg attaccgcac acttcaaatc
     7141 gtcgttcatg aaccagcaca agtgacactt ccgtcgctac cctggaccga gaacgcgcag
     7201 agcgtgcccg actagcccag tctcttcggc caggaaacgg cgcgaggacc cgtccgataa
     7261 cccgccaaat ctgagcgacg ggaggtcgag atgtcgggtg ataggacttg tacgcgtgct
     7321 tttgcatgct tgctctggct gcttggcgcc tgcgggcgag agacgttgct gatcgagcca
     7381 cgttgcgacg ggctcgcacc cggcgattta gtcatcaccg aggtgcacgc aaacccggat
     7441 gggagtgacg gggaggctga gtacatcgag ctcttcaacg cgagcccgga gcagctttca
     7501 ctcgacgggt tggtgcttgc aacgggtcgg gcagacggca cgggcgtcaa gacgcatcgt
     7561 ttccagggcg tatcgatcag cgaaggcgac ttcttcgtcg tcggcaatgc ggctccggat
     7621 tcgatgccgg cgcacctcga ttacagctac ggcacggcac tcggaagcct tcggaactct
     7681 gacgcgttcg tgtcggtcga gtgcggtgaa acgctcatcg atgagatgcg gtatgaggga
     7741 acgaacgacg gccgcgcgct cgagctcgac ggaggcttgg caccgaacca cgagttgaac
     7801 gatgacacga atctttggtg cccgacgccc gaaggtacgg acgagttcgt ccctggcaac
     7861 atcggaacgc ctggagcccg aaacagctcc tgcgctccga ttgagctcga gggtaactgc
     7921 ctggatgaca acgttgtccg tgcaagccgc tcgcctgggc cgggtcaagt gcagatcacg
     7981 gagtggatgg ccaatccggc cggtccggat gccgacttcg aatgggtcga ggtgttgttc
     8041 ggcgaagccg tcgacctcca tggattccaa ctgggtccga gtcccgactc actcgaagct
     8101 gtcatcgatg gagaagactg ttttccagtc gacgcgggga gacgggtcgt cttcggagcg
     8161 agtccggcgg cgaccccccg cgtcgacgcg gatctcggct tcagcttggg caattctgga
     8221 gctcgcagcg tcgttgcggg cgcgaacggc cgtgtcgtcg atcgagtcga ctacgatgga
     8281 acggtcgaag gggtcgcttg gcaaatcgac ccggtcggcg agctttgtct cgccagctcc
     8341 gacgaggagt acgccaccgg aaacctggga acgccgggag aggccaacac gttctgcccg
     8401 caggtcctcg acccaggaac ctgcctcgaa gacggtgtcc cgagaaagat cgtgagcccc
     8461 aaagccggcg aggcctggat cagcgaatgg atggcaaacc cttcgtcggt cggcagtcgt
     8521 gaaggcgagt ggctggagct ccgattcgac gcggaagtgg atctgaacgg gctcgtgctc
     8581 acagatctca cgtccagcgt gactcggatc gaccagaagg actgtatccg cgtcagtgcg
     8641 ggatcgcatc gcgtcttcgc gcggagcagc gactcgagtg aaaacggtgg catcacagga
     8701 gtgcttggcg agctttcttt ttctctcaac aatagcgatg aaacgatcac gctgagcgtc
     8761 gaagggcaga ccctcgatac ggtgagctac gagcgatcga ctctcggcgt ggccacccag
     8821 gtcgacgagc tcggcaagcg ctgcgatgcg atgcaccgct acggcgacgg cgatctcgga
     8881 acgccggggc tgccgaatcc ctggtgctcc tgagatccag cctcgtattc gcgtgcgtcc
     8941 tcgcgctcgc cgcacgaagc gctcgggccg cccagtacga ggtattcatc gacgtacaaa
     9001 ccgaggaaga cctctacgac ctgctcgtta cgggtcagat ctccaagtcc tcgttcgagg
     9061 cgctcttgct actgtatcaa acgcgggtag atctcaaccg cgctgggcgg ccgcaactct
     9121 atttgcttcc caatctcgac tacgatcagg tcgatcgaat cctcgcatat cgcagggagg
     9181 ccggctcgat tcaccagctc gaggacttgg tcgagtcggg cgcgctcgaa cccgagctgg
     9241 tgcgtgcgct tggctccttc gtgatcgtgc ggcccgcaga tgctccgcgg tcgagagtcg
     9301 acggtgtctt gcgcatccaa gccctttggt ccggccggaa cgatcgactg ccgcctgcga
     9361 tggcgttgca agcccgcgtc aaggctgctc gaaacctgga tgccggcatg gctgccaccc
     9421 tcacacgcaa tggattgggc cgcgtccggt gggatcgtac ccgtggcgcg ctcagcgccc
     9481 aacccgaacg cgcgcgcatc acggcgccga agctgtacgt agcttggagc gatgcgcgct
     9541 gggaggtcat cgccggaacg taccgaatcg gcttcggtca acgcctcacg ttcgacgtca
     9601 cggaccagat cacgccgaac gggttcattg gagactatgg gctcaggcgg ggcggggagc
     9661 tcgagttgcg ctgcaaacgc gccgctggcg agctgtatga gtctccgtgc tcgcgcgaaa
     9721 gggtccagcg tgtcactccg gacttcgact ggtcgaatcg gctgtctggc atcgcgcttg
     9781 gtctgagaga ccaggcgatc ggcaaaggct ggctgcaagc gtatgcctgg ggctcctatc
     9841 aggtccaccg ggccccacag atcgagcttg ccgatcggaa cctctgtgac gaccctgctc
     9901 gcgatgcaga cccggcatgc cgggccccag tggtctacgt tcgaggggcg gatccctctg
     9961 cgccgtccag tactgctacg ttcgcgagcc ttccggtgat gtacgcagaa ggcctggcgg
    10021 gtatgaacct cacgtacttc tggagcgacc gggcgcagct tgggctcacc gggtatggct
    10081 cggttccgag gtggctcatc gacggggcgg agctcggctt tcaagagttc gcgcgaaagc
    10141 cgttcgttgg cgctttcgga gcccttggtc tcaatgggtc ctttggattc ggcgcacagg
    10201 atttcttcgt agagctcgca cgaagcttcg atgcacttcg cggaagcggc ggcttgggag
    10261 cgatcgcgcg cagtgtgtcc acgcttcccg gagccgagat cgaagtgtcc gcgcgctatt
    10321 acgaaccgag ctatgccaac ccatatgcca gaccgatctc ggcgcccgat gagctcgatg
    10381 gtctacgggc gagggacgag gcgggactgc gtattcgggt gactgccgtt cggggccgcc
    10441 gcttcgggtt gcggaccgcc gccgacggat ggcggaggct cacttcgggt gcgtttggct
    10501 ccgtgctttt tgcccgcgca gacctgcagc tcgcttccgc atggacactc gcactctggg
    10561 ccgagcaccg caacacgggc gtgcagcgtt ttcttctggc gaccaagctc gcatacactc
    10621 ggggacggcg tgtcagcgtc tccgcacagc tcgagcacgc ttggagccaa atccgacttg
    10681 cagctggcgg ggcgcagcaa gacatcgcgg ccgtcttgtc gctcacgacc cagccacacg
    10741 agcttctgag gctcaggctg cgagtgcggt acgacttcga agacatctgg aacaatcacc
    10801 gtctgcctca ggcggtctgg ggctacctca acgcggcgct gaccctgcgc gagcgcgggc
    10861 tattgcgaat ccgatatgac ctgcgcgcct tcctggacga acgagggtcg acctctgccc
    10921 gcgctcccaa cccggagcac tggatttggc tcgagtacgc ctttcgctac taaagtggag
    10981 gcgctagtct cccggcgtgg gaaccttcgg aaaccagatc gaagctgcgc gtgtcttgta
    11041 ccgcgcgggc gttttcaaac cgattcggcc ggacaagacc ctgcgcatca tcaaggccgc
    11101 aaagggttgg gggcgctcgc ctgcgatggg gttcatcgcg ctggcgattc gtcagcccga
    11161 tacggtcgcg gtcatcgacg atgaaggcga agccactttc gacgaaatca atcgccgctc
    11221 gaacgcgttg gctcggggca ttcaggaagc cgggatctcg ccaggggacg tggtcggggt
    11281 catgtgccga aatcatcgcg gcttcgtgga gaccctgatc gcgtgctgca aggtcggcgc
    11341 gaacgcggtc ctgctgaaca cgatgttcgc cgctccgcag ctcgccgagg tgggcagacg
    11401 cgaaagcatt cgtgcgctgt tctacgacga ggagttcggg acgctgatcg agagctttcg
    11461 gggcatgcca cgattcgtcg tctacggcgc gggtgtcgac cctgcaatcg gcgaagtcat
    11521 cgcgaaccac gcggaccacg acccggagcc accgagtatc gaatcgcgtt tcaccatgct
    11581 gacttccggc accaccggaa cacccaaagg cgctaagcgc ggctctccaa aaggcatcct
    11641 tcccatcgcc tcggtgctgt ctaggttgcc tctgcgggcc ggcgaacgga cgatgatcgc
    11701 ggcgcctctg ttccattcct ggggccttgg gcagtttcag ttggggctct tgctgggcac
    11761 gacttacgtg ttgaaccggc gcttcgatcc ggaagcgacc ttgcgcgccg tctccgagca
    11821 ccgatgcacc tcgctcgcgg tggttcccat catgatgcaa cggatcctgg cccttcccga
    11881 cgacataaag cgcaagtacg acctcagctc gcttcgcgtt acggccgcaa gcggctcggc
    11941 tctaccaggc caccttgcga ccgagtggat ggacacgttt ggggacaatc tctacaattt
    12001 ctatggctcg acggaagtcg cgatggcgtc catcgcaacc cccgaagaca tgcgagccgc
    12061 gcccggaact gtgggcaagc cccctctggg cacatcggtg gcgatcctgg acggccaagg
    12121 ccaacggctc cccaacggag agaccggaag gatcttcgtc gccaatgcga taacgttcga
    12181 gggctacacc agcggagacg acaaggagcg cctgggtggc atggtgagca gcggagacct
    12241 gggccacttc gatgacgatg ggcgcctctt cgtcgacggc cgagacgacg acatgatcat
    12301 ctcgggtggc gaaaacgtct tcccccgcga agtcgaagat ctgatcgcca gccaccggag
    12361 cgtggcagag acagccgtca tcggagtgtc cgacggagaa tggggtcagc gactcaaagc
    12421 tttcgtcgta ctgcggtccg gcgcggattt gagtgaagac ggcgtgaggg tctatgtgaa
    12481 acagcggcta gcaggctata aagttccccg cgacgtggta ttcatcgacg ctttaccgcg
    12541 caacgcaacg ggcaaggtac tgaagcggga gctcgagcaa cactagcgat cgagcggcag
    12601 cgtctcgccg attgctggaa tgaatcgctg atcgtcgtgc agacccgctc gctcccagac
    12661 atctcgaagc aactccggtg gctcccccag atgctcgtcg gtgagcttga aggtgcccca
    12721 gtgcatggca acgaacttct cagatccgag cgctgaaaag gcacgcaccg catccccggg
    12781 gtccatgtgt tgagatctca tgaaccagcg cggcgcgtag gcgccgatcg gaagcatgga
    12841 ggcgtgaatc tctccgcagc gtgcccctat gagtgagaag ccttcgaacc aggcggtgtc
    12901 cccggagtga tagacgcgta ggccgccacg ttcgatcacg aacccacccc accaggtcgt
    12961 gttcatgtcg gtcaggcccc gacggctcca atgctgggag ggaacgaagg tcacccgtag
    13021 tccttcgatc tcttcttctt gccaccagtc catctcgacg acgcgtcgaa accccgctcg
    13081 ttcgaaggag gttccgaggc cgcgagggac cacgtacacg ggatcggggc ccaggcgttt
    13141 gagcgtgggt acgtccatgt ggtcacggtg attgtgcgtg atgaacacca cgtcgatctt
    13201 cggaagtgcc gaccaggcca atccgggcgc gacattgcgc tgaacgactc cactcaacct
    13261 ttgcgacatc accgggtcga tgagcgcgga tctccccccg agctgaacga ggaacgtcgc
    13321 gtgccctacc caggtaagcg catctcgaac ggcgtgccgg agcgccttgc cgtcgttgga
    13381 gacggctgga accggtatcc cggtggccgg ggtgcgcggg cgcttctcgt cgtgaaagcc
    13441 gagctgccac ttcaagatgc ggctcaatcc agcggcgttg gaagtcccgt cgaggttgga
    13501 gaacggcccg gccatgctga gaggtatagg gccccctagc cggcgaacaa tccttgcagg
    13561 aacttccgta gctcggccca actctgctcg tctgctgatt tatcgtaggc cagcggcatc
    13621 ttgagcttct gacctcgctc ggttgcctcg gggttcgtga acgcatgcgt tgcgccgggg
    13681 tactcgatga cgctgcagtc cacaccggcc gcctccattt ccttctcgaa agcagcgagc
    13741 tgctccgccg ggaccagtgg atctgcagcg ccatggagca cgagcacctt cgctttcaca
    13801 gccccgggct gcgcgggcgg gccctgaggc gacaaggtac cgtgaaagct cgccacccca
    13861 gcgagatcgg cgccgcgccg ggccatctgc aacacgacgg ctccgccgaa acagtatccg
    13921 atcgccgcga tcttcgaggg gtcggtcgct gggtgcttct tgaggacctc acacgccgca
    13981 aagaaacgct gctcggcaac agccggatca ccgaccgccg ccatcaagag tttctgagct
    14041 tcctcggggc gctgagtgtt cttcccgtcc ccgtacatgt cgagcgcgaa cgctgcgtag
    14101 ccgagctcgg caagcattcg cgcacgtcga cgaacgtact cgttgtgccc ccaccactca
    14161 tgcaccacga tgatgccagg ccgcgggcct gttttgctcg catcccaggc gagatagccc
    14221 tcgagcctgg taccgcccgc ttgataggac actgcctcgc cccgaatgtc gggcgcgttc
    14281 gctatttcgt tcatggcgat ctcctcgagc tcgagattag cgccgaaacc cgcagggcgc
    14341 ggcttacttc ttcttcttcg ccttgcgctc tgcgacctcg atctggacct tctcgaggat
    14401 gttgcgtgga accggcgagt agttgctgaa ctccatcgag aactgaccgc ggcctgacgt
    14461 ggcggaccgg aggtcgccaa tgtagccaaa catttcgctg aggggcacgt cggccttgat
    14521 ccggacgttg gtgtgtccct tttcctggga gttcacgatc cctcggcggc ggttgagatc
    14581 gccgatgacg tcgcccacgt tcgcgtcagg tacgtagacg tccaccttca tgatcggttc
    14641 gagaagctgc ggtccggcct tgggcatgct ctggcgaaag gccgcacggg ctgccgtctc
    14701 gaacgccatc tgagaagagt cgaccgcgtg gtagctgccg tcggtgagcg acaccttgaa
    14761 gtcgagaagc gggaacccac agagcggccc ctcatccttc atgaccttga agcccttttc
    14821 gaccgccgga atgaactcct tggggatgtt tccgccggta atcttggact cgaagacgaa
    14881 gccttcaccg ggctcggccg gctcgatcgt gtagtcgatc tttgcgtact ggccggagcc
    14941 accggtctgc tttttgtgcg tatagctgtc ctgaatcgcc tgcgtgacgg tctcccggta
    15001 cgcgacctga ggcgcgccga cgtcgaccgc aacaccgtgg ctacggcgaa gaatgtcgac
    15061 cttgatgtcg aggtggagct cgcccatgcc cttgaggatg gtttcgccgg attcctggtc
    15121 gacctcgacg tgaaacgagg ggtcctcggc gaccattttg ccgagcgccg tgccgagctt
    15181 ctcgctgttg gccttgtcct tggtgctcac cgcaatcgag atcacggggt cgggaaagac
    15241 catcggctcc agcgtagccg ggttcttctg atcggcgagc gtatgccccg tgcgcacgtc
    15301 tttgagacct accatggcga cgatgtcgcc agcctgcgcc gaatcgatga tggtgcggtc
    15361 gtcggcgtgc atctctacca tgcgcccgat tcgctctgac ttaccggtga atgtgttgat
    15421 gagagtgtcg cccttgttga tcgtacccga atagatccgg gtgaatgtga gggcgccgaa
    15481 gcgatcttcc atgatcttga acgccagcgc tcgaacgggg ccatcgggat cgacgatcgc
    15541 gaacttgccc gtctcgttgc cctctaggtc gatttcgggc tgcggaggaa cttgtgtggg
    15601 gtcggggagg tagtcgacga cggcgttcag aacgagctga atgcccttgt tcttgaatgc
    15661 ggacccgcag taggtcggga agaagacgag gtcgcgcgtg ccgatgcgga tgcacttctt
    15721 gatggtttcg agatcgggtt ccgttccttc gaggtattgc tccatcgcgt cgtcgtcctg
    15781 ttcgacggcg gcctcgatga gctcgaagcg gtacttgtcg accaactcct tcatgtcctc
    15841 ggggacttcc ttcacctcgt agttctcggg ctgtccggaa tcgtcccaaa cgtaggcctt
    15901 gcgcgtgaga agatcgatga cgccggagaa atcaccctcg gtcccgatgg gaagcaccat
    15961 gacgagcggc ttggcgccga gaacggactt gatctgcccg acgacgcggt agaagtccgc
    16021 gcccaaccgg tcgagcttgt tgacgaaaat cagccgagca accttggaat cgttagcgta
    16081 gcgccagttg gtttcggact gtggctcgac accgccggaa ccgcagaaca cgccgacacc
    16141 accgtctagc accttgagcg aacgatagac ctcgatcgtg aagtcgacgt gaccgggcgt
    16201 atcgatgatg ttcagttggt ggccgttcca gaagcaggtg gtcgctgcgg actgaatggt
    16261 gatgccgcgc tcctgctcct gctccatgaa atcggtcgtg gccgcgccat cgtgcacctc
    16321 accgatcttg tggatcttac cggtgagctt gaggatgcgc tcggtggtcg tcgtcttgcc
    16381 ggcgtccacg tgcgcgaaga tgccgatgtt tcggtattta ctaaggtctt tcattctttt
    16441 gctcgtcaga ggagtcgttt tgccacgcgc cggataccgc gaaagcaggc gcatgcaagc
    16501 ccacgaccgg cagtagaccg gaatttgctg cgaaaacgcc cccaagtgct cgtctatatc
    16561 gctcgaattc gtgccgtgac tgaccgtgac gctggcgagc gtggcgagct ggccaagcga
    16621 cagggttcgc gtgcgagcga acgctactcg tagagcttga tgactttgac gccttcgggc
    16681 gaggcctgca tgcacagatc gcagagggtg cattcgtcga cgttctcctc gacgacctcg
    16741 acggcgttcg acttcgatcc gagcttgaag atcttgacgg ggcacacctc gacgagcttc
    16801 gcggcgaggc caggggtctt ctccacgact ttgtggtcga catctacgcg tatgaacatg
    16861 gccatgatgg ttcctctgcc gctcagtttc cgattcgctc gcggacgagg ttgggaatgt
    16921 cgccgatctc ttcggcgacc gaaatacccg cctcgcgcat ccggcgcatc ttgtcggcag
    16981 gcgaatcgac cttcttgttc acgacggttc ccgcgtgtcc aaagctcatg ccaggcattt
    17041 cgtccatgaa gcggcccgcc acgaacgcca cgattggaag acggctcttg tgctcctggg
    17101 tccagcgcgc gagctcggcc tcggccgtgc ctccgggctc ggagtagacg accaccgcct
    17161 tggtatcctc atcggcctcg aagagcggta tcaaatccgc gtaggtcgag ccgatcaccg
    17221 ggtcgccgcc aatcgaaatc gcggtgctga cgccgagccc cgagagggtc agggcgttgc
    17281 agatctcggt ggtcatgccg cccgagcgcg acatgacacc gacgaccccg ggcttgaagg
    17341 ccttgcggca atcgaccgcc ggtccaccga ggcttccaaa gcgacagacg tccgggacga
    17401 tcaacccgag gcagtttggg ccgatgagcc gggccccatg aagctccgca tactcgacta
    17461 tcgcggatgc atctcttctc gggatgccct cggtgatgat gatgatctct ttgatgccag
    17521 cgtcgatcgc ttcgtatgcg gcgtccggag caaaggcgag cggtactgtg atgaccgttc
    17581 catcgactcg ctctttctcc gtgatctcgc ggaccgtgtc gtagaccggg acaccgtaaa
    17641 cctcgcgccc gcccttccct ggggtgacgc caccgatgat cttcgagccg tactgcaagc
    17701 attcgcgggt gaaattcagc gcctcacgtc ccgtgatgcc ctgcacgatg aattggaagt
    17761 cttcgtgaac gataacggcc attgatcttc cctctagccc actttctcga cggcgcgcct
    17821 cgccgcttcg ctaatggaaa ccgtgcgatc gcagtattcg acgccgtact tctggaggat
    17881 cttcgcagcg tccgcttccc aagccccagg caccctgaag atcaaaatct tgtcggccgg
    17941 ctcgaaaccc aactccttga tccccttgat gacgccgcgt gcgacgagat ccgcgcgggt
    18001 gttggaaacg acgttggaca tgacggcgat cttctcgacg ccgggcttgc tcagaacgag
    18061 cttggtcagc cttcgcgcct tgtcgacgct ggggtttccg ccgatcgcgg cgtagtttgc
    18121 gggccttccg ccatgctttc gcacggcatc ggtcagcgtg agcgaacctc cgccggcgcc
    18181 gatgacgaga ccgatgttgc catcaaactc cgcgattggg ctgatgatac cccgtggatc
    18241 ctcggcatcg attcgcgcac cctcgagctc gaactcggtc ggttcacgca cctgccggag
    18301 gtcatccttc gaaaaaccca gctcatcgag catgggtcgt tgccgatgac gtgcttcgat
    18361 ctcgaggtcg gcctcgacgt cgcagacgac gaagtttccg tccgcagtct tcgccatgga
    18421 cgtgatgtcg aggtctgcca gatcgtactc gaggaaaagc cgggcaagcc ctgacacgat
    18481 gcgcgtcatg cgattgaggt cgctgccgcc gagacccagc gacttggcga gctccttggc
    18541 gatgtagtcg gagaacggaa tgagcgcgga gaactgccga cgtacgattc gatcaggatg
    18601 cgtgtcgcct agatcgtcga tctttcccgc catggcggcc gcggcgacga ccgggcgctt
    18661 gctcgaaccg tcgtacgtga agctgagact gtagaccgct tcagcgactg gtttggcctc
    18721 gacgagcaca ccgttcggct tccggcctcc gtcgtcgaga gcaagcaact cagctgcgac
    18781 ggccttcgcc gcagctgggt cggccgcgtc cctcaccgag gcggcggcga gtcctggtgc
    18841 aatggcttgc gccttgacca cgactgggcc gccgatgtcg gaggcgatct ttttcgcctc
    18901 atcggctgtc tttgcaagtt cgcctcgaac cagcggaatc cgatgcttct tcagcagttg
    18961 cttcgcttca aattcgtaaa agcgcatagt ggaacgggga gactatccaa tcttcccgtt
    19021 gccgtctacg gcgcaattgc ttcggccaag gcaacgacgc gctccgcatg gtcgacgtgc
    19081 aacctttcga tgagcttgcc atcgaccacg accacgcctt tgccctgggc ttcggcctcg
    19141 gcataggcct cgatgatgcg tctggacaaa cgaagctcct cttcggaggg agcgaatatc
    19201 tcgtttgcgg cctcgagctg cttcgggtgg atcagcgtct tcccatcgaa accgagctcc
    19261 gcgccctgac ggcaggaatc gacgaagccc tcgtggtcgt tcaggtcgag gtagacgccg
    19321 tctagaatcg tgagacgcgc cgcacgggct gcgagcagac atagccccaa ggcggtgatc
    19381 agcggaaggc gcatggcagt gtgttgtgcc tgcaggtcct tggcgagatc ggaagtgccc
    19441 atgaccagac caccgagccg cggactcgca tctgcgatct cttcggcgtg cagcattgcg
    19501 cgcggcgttt ccatcatgca ccagatcgcc aagtcggggg aagcgccatg cgacaccagc
    19561 accgactccg cctgccgtac ggtttccgca ctttctactt tgggcagaag caccgcatcc
    19621 ggtcgaacgg cggcagccgc caccaagtcg tcatagcccc aaggcgtgtt caagccgttc
    19681 gtacggatca tgacttcgcg tcttccgtac ccacctgcct tcaccgcgct cacgacctgg
    19741 ctgcgggcgg tctccttcat gtccggtgca actgcatcct cgaggtcgag aatcaagctg
    19801 tcggcgggaa gcgaacgggc cttctcgagt gcccgcgcat tagcgccggg catgtacagg
    19861 acactgcgtc gtgggcgatg cttcttgctc atcgtgacac ctctcagaac tggtaggccg
    19921 ccttcaggtc ggcatccttt tccgcgagca ttcgggcgag ctcgaccatg accttgcact
    19981 gtttgacagt agcgtcgtct tgcatcttgc cgtcgatcat gacggcaccg gtgccatcgc
    20041 ccatctcgcg gatgaccttc ttggcccaga gcacctcgtc gaccggcggg ctgaagacct
    20101 tcttggcgat gtcgatctgg accgggtgaa gcgaccaggc gccgacacag ccgaggagga
    20161 aggcgttgcg aaactggtct tcgcacgcga ccacgtcgcg gatgtctcca aatggcccgt
    20221 aataggcaaa gatgccgttc gcgacgcagg cgtcaaccat gcgcgcgatg gtgtagtgcc
    20281 agaggtcttg ctgtgcggtg gcccgcggcg cttcgggatc gtctacatca ggatcctggc
    20341 gaaccaagta acccgggtga cctccaccga cgcgggtcgt tttcatccgc cgcgaggctg
    20401 cgaggtcggc cggcccgagg ctcaggccct gcatgcgcgg gctcgcgcca gagatctcct
    20461 cgacgttggc cacaccgagc gcggtttcga gaattgcgtg aatgagaatc ggcctcttga
    20521 ttccggcgcg cgcctctagc tgggctagga gacgatcgac gtagtggatg tcccagggcc
    20581 cttcgacctt gggaatcatg atcacgtcga ccttgtcgcc gacctcctgg acgatctgcc
    20641 acatgtcatc gagaaaccag gggctatcga ggctgttgac gcgggtccag agctggcagt
    20701 cgccgaactc gttctccttc gcaatcttca cgaagccttc tcgagcttcg gtcttcttgt
    20761 ccgccgagat ggcgtcctcg agattgccga gaagcacgtc gcacttcgcg gctatactcg
    20821 gtgccttcgc agccatcttg ggattgctag ggtcgaagaa atggatcatc cgcgacggcc
    20881 ggaacgggat ctcccttggg ggttccggtg cgccgatggc catgggtttg aagaagtcct
    20941 tgggattgcg catgttaccc ccagtcctag acgccaagtg gcggagatgc aacgagcgcg
    21001 caggcgcgac tagaggagca gatggaaatc ggtatgaacc ttccggtgat ggcgccggga
    21061 ctcgacaggg acacctttct ggcctggtgc gagcgaatcg acgccgggtt ctttgcgagc
    21121 ctggcggcag gtgagcggat ctcgttctac aacccggaca tgaccgcggc cctggccgcc
    21181 gcctccgtcc tcaccaagcg cgtcaagatc gtgagcggtg tcttcgttcc gatgctccac
    21241 catccggtca tgctagccaa gcagctcgcc acgatcgacg tgctctcggg agggcgcttc
    21301 gtcgccgggc tcggggtcgg cgggcgcgag caggactacc agtcggtcga tgctccgctt
    21361 ggcaaacgcc tcacgcgact cgccgagagt gtcaagacga tgcgcagcgt ctggcgcgga
    21421 gagccggtcg tagagggcgc gcttcccgtg ggcccggcac ccgttcagga cggcgggccg
    21481 aagcttcttg cgggctcact catggtcgag tcgatccaaa cggcctcgcg ttgggccgat
    21541 gggctctgtg gattcagttt cggaccctcc cctgctgaaa tggagttgca gttcggagcg
    21601 gcgcggcgtg cttggaaaga tcggggacgg ccagccccat ggcttggaac cgggttctgg
    21661 tatgcgctcg gtccgaacgc acgagcgcaa ctggacgcct atctcgatcg ctatctcaat
    21721 ttcatggcgc ccgtggcgcg cgagagcgtg aagcagatct gcatcgcgac gacgcctcag
    21781 gccctgaaga acgccattca gcaggccaag gacgcgggcg ccgacgatgt cctgctcgtc
    21841 cccactacgt cggaccccga cgatgtgcat cgcgtgaccg atatccttgg ctagaaacac
    21901 gccctcagag cttgagtcgc gtgttataag cgcggttcag aggaggtacg tcatgagagc
    21961 catcatcgtt gccatgtttc tggttggagc gttcgtcgca ggactgctga tcgcagaagc
    22021 gaacccggcc gcagccgacg gaccgcccaa gaaggcccaa tggcagtacc agtgcttcga
    22081 ggcggacggc gtagcgcagg tcaccgagcg cgccaacggg atgggtgaac agggttggga
    22141 gctcgtaacc tccgctggga ccgtgaaagg cccgacactg tggtgcttca agcgaccgct
    22201 ttggaagcct aagaagtaag cgcttggtcg ggctcgcgtt ggcgagcaag ttgagctatt
    22261 tgcccctcag ccgaggatcg cctcgcccag acgatgtcat tcctggatga tccacaagtc
    22321 aggcggaacg tagagcgcct tcccatcgag ttcgacgact acggattcga tcgtttcgga
    22381 ctctcgcgaa aagccctgat acgcgcctac tcgcccatgg cgtatatcta ccgtcgctat
    22441 ctcaaggtga cggcgtttgg gctcgagaac gttcctgcga aggggcgagg gctcgtcatc
    22501 gcgaaccact cgggcggcct cggcgcagac gcggccatga tcttcacgtc gttggtactc
    22561 aatgacgagg cgccaaggct cggacagggc atggcggagt acttcctcac gcgatcgcca
    22621 ttcgccgcgc cggcgctgcg taagctcggc cacctcaccg gactaccgga gcatggcgag
    22681 caactgctga acgacgagcg cctggtggtc gtcttccccg aaggcgcccg aggggcgggc
    22741 aagctctaca aagatcgcta caagctcgtc cggttcggaa ccgggttcat gcggctggcc
    22801 ctcaagaccg gagcgcccat catcccttgc gcattcatcg gcggagagga gtcgttcccg
    22861 cagctgtttc acatcaagtg gctcgcgaag ctcgtcaatg gaccgttcgt cccagtagcg
    22921 ccgcagctca ttttctttcc gctgcccgtg gcgtgccagg tttactacgg gcccccgatg
    22981 cattttgagg gcgacggctc ggagccagac cacgtcattc agcaatacat cgaagaggta
    23041 cgtcactcga tggagcgatt gatcagcggc gggctggatg cgcgtccgcg agcgttcatg
    23101 ttcgaacgca tgcccgacgc ggagaaagaa cgcaggccat gaagatcttg atcacgggga
    23161 tcagcgggac cttggcgcga ggcgtcgctt accggctggt ccgtcgaggg taccaggtcg
    23221 tcggcatcga ccgccgtccc tggccggacg taccggaagg cgtcgagatg caccaggccg
    23281 acatccgaaa gcgccccgcc gaggacgtgt tccgaatttc tcgcccagat gcgctcattc
    23341 acatggccac ggtcacacat ttcacgaccg acaccgaaga gcggtaccgg atcaatttgg
    23401 gtgggacccg caagctgttc gagtactgcc acgagtacgg ggtcaagcag gccgtcttcg
    23461 tcggccgcca caccgtctac ggcgccaccc cggattcacc tctctaccac accgaggatg
    23521 agcccccgct cgcgggcgcc gctttcaagg agctggcaga cctcgtggcg gccgatctct
    23581 acgccggcag cgcgctttgg cgctggcccg agctcaacac ggctgttctc cgcctttgct
    23641 acttcctcgg gccgagtcga ggcgggacct tggcgagctt cctctcgaag aacagggttc
    23701 cgacggtgct cggcttcgac cctctctacc aactgatgca cgagtgggac gccgaagaag
    23761 cgatcactct cgccgtcgag aaccagctcc ggggcgtctt caacgtagcc ggcccgccac
    23821 cggtgccact ct
//
LOCUS       DASRZF010000440         8188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_2749352, whole genome shotgun sequence.
ACCESSION   DASRZF010000440 DASRZF010000000
VERSION     DASRZF010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 8188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8188
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2749352"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..1362
                     /gene="tig"
                     /locus_tag="VFG22_19340"
     CDS             31..1362
                     /gene="tig"
                     /locus_tag="VFG22_19340"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HET6418461.1"
                     /translation="MQSEIREISPVLVEVTVEVPWDRIQKDLNDTYREIGRTARVKGF
                     RPGKVPRTVLKQVYGKQAAAQVAGTLVEQGLMQAVQEHELHIVAQPEIDEAPELQQGK
                     PLTFKAKVEVRPKIDSVAIDAIEAWQQKIDVPDEDVDAEIESLRNQHAEIQAPDPMRA
                     AREGDLITIDYTVTVDGEAKDDLGAEGRQVELSDEHLLPALKEGLLGMQPEEEKTIEV
                     AFEDDHPNPDLQGKTAHFKIKARQLHEKLLPELDDEFAKDCGDYETLLELRLKIREGL
                     EDGAKRRNEAQLKDQLIDSLIEHNDIPVPPSMVQQQQQMMMYELAQFMQMAGQQGAPG
                     ADFFSGIEKRAERRVQAGILLGSLARQEGIEVTEADVDAKFQEIAEKTGKHVAKVRAD
                     FQEGQREQLESQLLEEKVMALLAERAKINEGERPAPKAAAGETPDDEGEES"
     gene            1383..1970
                     /gene="clpP"
                     /locus_tag="VFG22_19345"
     CDS             1383..1970
                     /gene="clpP"
                     /locus_tag="VFG22_19345"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HET6418462.1"
                     /translation="MYMPWVVETTHRGERQWDIFSRLLKDRIIFLGSEVNDDMANAII
                     AQMLFLESDDPDKEITLYINSPGGSITAGLAIYDTMQYVRSPVSTVCLGQAASMAAWI
                     LASGTTGLRKCLPNSRIMIHQPLGGIRGQATDIEIHAREILKLREQMNTILSKHTGQS
                     VERIKDDTERDRYLSAEEAKEYGLIDSVIQATPGK"
     gene            2053..3312
                     /gene="clpX"
                     /locus_tag="VFG22_19350"
     CDS             2053..3312
                     /gene="clpX"
                     /locus_tag="VFG22_19350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HET6418463.1"
                     /translation="MVDRRRDDGHGNLQCSFCGKSQKEVKKLIAGPTVYICDECIGLC
                     NDIIAEEVEREESLTTTTPLPKPAEIKKILDEYVIGQDRAKKILSVAVHNHYKRIDSK
                     ITADDVELQKSNILLLGPTGSGKTLLAQTLARILNVPFAIADATTLTEAGYVGEDVEN
                     IIVQLLQNADHDVERAQRGIVYIDEIDKVARKGDNPSITRDVSGEGVQQALLKIIEGT
                     VANVPPKGGRKHPQQDFLQIDTGNILFICGGAFVGLDDVIERRIGERTLGFGADIPSR
                     KDKKIGALLERIEPEDLLRAGLIPEFIGRLPVIATLHELNEEALVDILTKPRNALVKQ
                     YQRLFEMDGVKLKFSKGALHAVAREALKRNAGARGLRAIMEKSMLDIMYDVPSQADIR
                     EVVVSEEVILSGEMPLIVYEKEEAELA"
     gene            3370..5874
                     /gene="lon"
                     /locus_tag="VFG22_19355"
     CDS             3370..5874
                     /gene="lon"
                     /locus_tag="VFG22_19355"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HET6418464.1"
                     /translation="MFFHRNENDEQNPGGDESGAESYPLLPLRDIIVFPHMVVPLFVG
                     REKSINALEEAMSQHKEILLAAQKKAKTNDPTPEDIFEVGTIGTIIQLLRLPDGTVKV
                     LVEGKRRARITQFVPSDEFFLCNVEPVEEVSSSNIEIEALIRSVQSAFDVYAKLNKRI
                     APEMLMTVQTIDDPSKLADTVVVHLTSIKLSDRQEILETIDPAKRLERLFQLMNAEIE
                     ILQVERKIRSRVKKQMEKTQKEYYLNEQMQAIQKELGERDEFKSEIQELETQLKQKKL
                     SKEAHLKARKELKKLKMMQPMSAEATVIRNYIDWVLALPWGDKSEENYDIDMAEQILD
                     EDHYGLKKPKERIVEYLAVQALVKKLKGPVLCLVGPPGVGKTSLAKSIARCTGREFVR
                     LSLGGVRDEAEIRGHRRTYIGALPGRMIQSLRKVGANNPVFLLDEIDKMSADFRGDPA
                     SALLEVLDPEQNHSFNDHYLDLDYDLSDVMFITTANTLQGIPLPLRDRMEILEISGYT
                     EFEKLNIAMRYLVPRQRTNAGLQDIDLEVSENAIRQIIHYYTMESGVRNLEREIGSVC
                     RKIARQVLKDGEDSKGKSYRVEAKDVAEYLGVTKYRPDKTNEKDHVGLTNGLAVNAYG
                     GSILECEVAVVPGKGKLNITGLLEKGMQESAQAAMSYIRSRQKMLGLEVDFHSKYDVH
                     IHFPSFVPKDGPSAGITMATSIASALLKIPVRKNVAMTGEITLRGRVLPIGGLKEKLL
                     AAHRAEIDVVLVPKENQKDLKEIPRRVLNALRIVLVEHMDEVLREALALSDPDALFGE
                     HQVRPVEYREGELFTPEEAETEDVPEPTVDAPGARQ"
     gene            5930..6001
                     /locus_tag="VFG22_19360"
     tRNA            5930..6001
                     /locus_tag="VFG22_19360"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5962..5964,aa:Val,seq:tac)
     gene            6126..6776
                     /locus_tag="VFG22_19365"
     CDS             6126..6776
                     /locus_tag="VFG22_19365"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6418465.1"
                     /translation="MAVAARRERERTARREAILDAAQELIAEEGYHGLRMDAVAEAAE
                     LSKGTLYLYFGNKDALCAAVAIRLLDTLIPVITSALENTPTGLDAVRQLLRSYYDFTQ
                     KNPHHFRFALAWLSAGERMDDSTEEFQSYRARVGQMLILVTSALKRGQDDGSIRAEVD
                     PLPQALQLWTSFLGVVLVGLNKEAMAQRIPASVDLDQLVPLHLDTMLRALSSEGTS"
     gene            6776..>8188
                     /locus_tag="VFG22_19370"
     CDS             6776..>8188
                     /locus_tag="VFG22_19370"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET6418466.1"
                     /translation="MRIAIPVLLASALIAGCNYKKETDIPPPLEVPESFSEGSEVAPP
                     EPEADALPEGKSGSETIRDVRWWRQMGDPTLTALIEEALRRNQTVRAGWARVQQSQYI
                     ANLVRAARMPTIGAVGAFNAAKNITPFAETTSLDAQGSIPVAYEVDLFARRAREHQAA
                     KLDAEAARLDQEALAIAISASVAEAWFDSVNARIQNAVVAEQLETDLKFLELVELRYR
                     EGLNSAVDVHQQRQRVAGQRALLAVADSLMELTDQRLSVLLGEAPGREFPVDGKTLPE
                     IGPTPAIEVPASLLDARPDIRAIQKRVEAEDRRVAAAIGARLPQVVIDFTPSYTWLRS
                     KITSTGALGGVPGETTDPITASGFTWNTGATFSVPLFDGIAGRSQIAANRAAVDALLE
                     TYAETILTALLEVESSLVLERQERLRIQFLEEEFSLANITLEATRDRYRAGLSDFLPV
                     LTALATRQTSELQLVASRRQL"
BASE COUNT         1845 a         2504 c         2489 g         1350 t
ORIGIN      
        1 aaccgcgcct gagaaacccc gaaaaatacg atgcaatctg aaattcgtga gatcagcccc
       61 gtcctcgtgg aggtcacggt cgaagtgccg tgggaccgaa tccaaaagga cttaaacgac
      121 acgtaccgag agatcgggag aaccgcccgc gtgaagggct ttcggccggg caaggtaccg
      181 agaaccgtcc tgaagcaggt ctacggcaag caagccgctg cccaggtcgc cggcacgctg
      241 gtcgagcaag gcctcatgca ggcggtgcag gagcacgagc ttcacatcgt ggcgcagcca
      301 gagatcgacg aggcccccga gcttcaacag ggaaagcccc tcaccttcaa ggcgaaggtg
      361 gaggtgaggc ccaagatcga ctcggtcgcg atcgacgcga ttgaagcctg gcaacagaag
      421 atcgacgtcc cggacgaaga cgtagacgcc gagatcgaaa gcctccggaa tcagcacgcc
      481 gagatccagg ctcccgatcc gatgcgtgcg gcacgagaag gcgacctcat cacaatcgac
      541 tacacggtga cggtggatgg cgaagccaag gacgaccttg gagcggaagg tcgtcaggtc
      601 gagctcagcg atgagcacct tctgcctgcg ctcaaggaag ggctcctcgg gatgcagccc
      661 gaggaggaaa agaccatcga ggtggcgttc gaagacgacc acccaaatcc agacctccag
      721 ggcaagacgg cccatttcaa gatcaaggcc aggcagctgc acgaaaaact gctgcctgag
      781 ctcgacgacg aattcgcaaa ggattgcggc gactacgaaa cacttctcga gctgcgattg
      841 aagatccgcg aagggctcga ggacggagca aagcgacgca acgaggcgca gctcaaggac
      901 cagctcatcg atagcctgat cgagcacaac gacatccccg tcccgccttc gatggtgcag
      961 cagcaacagc agatgatgat gtacgagctc gcgcaattca tgcagatggc gggtcagcaa
     1021 ggggcgcccg gcgcggactt cttctcgggc atcgagaagc gcgctgagcg tcgggtccag
     1081 gcgggcattc ttctcggctc cttggcgcgt caggagggca tcgaagtcac cgaagcggat
     1141 gtcgacgcga agttccagga gatcgccgag aagaccggga agcatgtggc gaaggtgcgg
     1201 gcggatttcc aagaggggca gcgagaacag ctcgaatccc agctactcga ggagaaggtc
     1261 atggcgttgc tggccgagcg tgccaagatc aacgaagggg agcggcctgc gcccaaagcc
     1321 gcggcaggcg agacgcccga cgacgaaggg gaagagtcat gagcgagcca acgaccgcac
     1381 agatgtatat gccctgggtg gtggaaacga cgcaccgcgg cgaacgtcag tgggacatct
     1441 tcagccggct cctgaaagac cgaatcatct tcctcggcag cgaggtcaac gacgacatgg
     1501 ccaacgccat tatcgcccag atgctttttc tcgagagcga cgacccggac aaagagatca
     1561 cgctctacat caatagcccg ggcggatcga ttacagcagg cctcgcgatt tacgacacga
     1621 tgcagtacgt tcgatcaccc gtttcgaccg tctgcctggg tcaggccgcg tcgatggcag
     1681 catggatttt ggcctctggc acgaccggat tgcggaaatg tttgcccaat agccgcatca
     1741 tgattcacca gccgctcgga gggattcggg gtcaggccac cgacatcgaa atccacgcgc
     1801 gagagatcct gaaacttcgt gaacaaatga acacgatcct gtccaagcat acaggccaga
     1861 gcgtggagcg tattaaggac gacaccgaac gcgatcgcta cctctccgcc gaagaagcca
     1921 aagagtacgg cctcatcgac tccgtcatcc aggcgacgcc ggggaaataa gacgccagaa
     1981 aacactgcgg atgcggggtg cgtagtcctt gccgttaaag atgcgggacg ctagactctt
     2041 ccaagcaggt atatggtgga tcgacggcga gatgacggac acggaaacct tcaatgttct
     2101 ttctgcggaa agtcgcagaa agaagtaaag aagctcatcg ccggcccgac ggtctacatc
     2161 tgcgacgagt gcatcgggct ctgcaacgac atcatcgccg aggaggtcga gcgcgaggag
     2221 tccctcacga cgaccacgcc gcttcccaag cccgccgaga tcaagaagat cctcgatgag
     2281 tacgtcatcg ggcaggatcg ggcgaagaag atcctctcgg tcgcggtgca caaccactac
     2341 aagcgcatcg actcgaagat caccgcagat gatgtcgagc ttcaaaagtc gaacatactc
     2401 ctgctcggac ccaccgggtc gggaaagact ctcctcgcgc agacgctggc gcggattctg
     2461 aacgttccct tcgcaatcgc ggacgccacc acgctgaccg aggccggcta cgtcggcgag
     2521 gatgtcgaga acatcatcgt tcagctcctg cagaacgcag accacgacgt cgagcgcgcg
     2581 cagcgtggca tcgtctacat cgacgagatc gacaaggtcg cgcgcaaagg cgacaaccct
     2641 tcgatcacgc gcgacgtgag tggtgaaggc gttcagcaag ccctcttgaa gatcatcgaa
     2701 gggaccgttg ccaacgtccc accgaagggc gggcgcaagc atccccaaca agacttcttg
     2761 cagatcgaca ctggcaacat cctgttcatt tgcggcggcg ccttcgtcgg cctggacgat
     2821 gtcatcgagc gccgaatcgg cgaacgaacg ctcggtttcg gcgcggacat tccgtcccgt
     2881 aaagacaaga agatcggcgc acttctcgag cgcatcgagc cagaggatct gctgcgagcc
     2941 ggcctaattc cagagttcat cggtcgtttg ccagtgatcg cgaccctgca cgagctcaac
     3001 gaagaagcgc tcgtcgacat tctcacgaag cccaggaacg ctctcgtcaa gcagtaccaa
     3061 aggctcttcg agatggacgg tgtgaagctc aagttctcca agggcgccct gcacgccgtg
     3121 gcgcgcgagg cactgaagcg aaacgccggc gcgcgaggcc ttcgggccat catggaaaag
     3181 tcgatgctcg acatcatgta tgacgttccg tcgcaggcgg acattcgaga ggtcgtcgtc
     3241 agcgaagagg tgatcctctc gggggagatg cctctcatcg tctacgaaaa ggaagaggcg
     3301 gagctcgcct agtgcgagcg tccctgcagt tcccggcttc tgcctcttca agaggagctg
     3361 agtagaccga tgttttttca ccgaaacgag aacgacgagc agaatcccgg cggcgacgag
     3421 tccggcgccg agagctatcc tctgctcccc ttgcgcgaca tcatcgtttt cccacacatg
     3481 gtcgtgcccc tgttcgtagg tcgcgagaag tcgatcaacg cgctcgagga agcgatgagc
     3541 cagcacaagg agatcttgct ggccgcccag aaaaaagcga agaccaacga tcccacgcct
     3601 gaggacatct tcgaggttgg gaccatcggc acgatcattc agctgctgcg cctgccggac
     3661 ggcacggtga aagtgctcgt agagggcaag cggcgcgctc gaatcacgca gtttgtcccc
     3721 agcgacgagt tcttcctctg caacgtcgag ccggtcgaag aggtctcctc ctcgaacatc
     3781 gaaatcgaag ccttgattcg ctcggtgcag tccgcattcg acgtctacgc caagctcaac
     3841 aagcgcatcg cgcccgagat gctgatgacc gtgcagacca tcgacgaccc gtcgaagctc
     3901 gccgacacgg tggtggtgca cctcaccagc atcaagctct cggaccgcca agagattcta
     3961 gagacgatcg atccggcgaa gcgcctcgaa cgtttgttcc agctgatgaa cgccgagatc
     4021 gagatcctgc aggtcgagcg aaagattcgc tcccgcgtca agaagcaaat ggagaagacg
     4081 cagaaggagt actacctgaa tgagcagatg caggccattc agaaggagct cggcgagcgc
     4141 gacgaattca agagcgagat tcaggagctc gaaacgcagc tcaagcaaaa gaagctcagc
     4201 aaggaggcgc acctcaaggc gcgcaaggag ctgaagaagc tcaaaatgat gcagccgatg
     4261 agcgcggagg ccaccgtcat ccgcaactac atcgactggg tgctcgcgct cccttggggc
     4321 gacaagagcg aagagaacta cgacatcgac atggccgaac agatcctcga cgaggatcac
     4381 tacggcctga agaagcccaa ggagcgcatc gtcgagtacc tcgcggtgca ggccctggtg
     4441 aagaagctca aaggccccgt gctttgtctc gtcggaccac ccggcgttgg caagacctcc
     4501 ctggcgaaga gcattgcgcg ctgcaccggg cgcgagttcg tgcgcttgtc gcttggcggc
     4561 gtgcgcgacg aggccgaaat ccgcggtcac cggcgcacgt acatcggcgc cctgcccggc
     4621 cgaatgattc aatcactacg caaagtcggg gcgaacaacc cggtatttct gctggacgaa
     4681 atcgacaaga tgtctgcgga cttccgcggc gacccggcct cggcattgct cgaagtgctc
     4741 gacccggagc agaaccacag cttcaatgac cattacctcg atctcgacta cgacctctcc
     4801 gacgtgatgt tcatcacgac cgcgaacacg ttgcagggga ttccccttcc gctccgtgac
     4861 cgcatggaaa tcctcgagat cagcggctac accgagttcg agaagctgaa catcgcgatg
     4921 cggtacttgg tgccacgcca gcgcacgaac gccggcctcc aagacatcga cctcgaagtc
     4981 agcgagaacg cgattcggca gatcatccac tactacacca tggaaagtgg tgtccgtaac
     5041 ctcgagcggg aaatcggcag cgtctgtcgc aaaatcgccc gacaagttct caaggatggc
     5101 gaggactcca agggtaagag ctaccgcgtc gaagcgaaag atgttgcgga gtatctcggg
     5161 gtcacgaaat accggcccga caagacgaac gaaaaggacc acgtcggcct cacgaacggt
     5221 ctcgctgtga atgcgtacgg cggctcgatt ctcgagtgcg aggtggccgt ggttcctggc
     5281 aaggggaagc tcaacatcac gggcctgctc gaaaagggca tgcaggagtc tgcgcaagcc
     5341 gcgatgagct acatccgttc gcgccagaag atgctcggcc tcgaggtcga cttccactcg
     5401 aagtacgacg ttcacattca cttcccgagc ttcgtcccca aggatggccc gagcgccggc
     5461 atcacgatgg cgaccagcat cgccagcgcc ctgctcaaaa tcccggttcg caagaacgtc
     5521 gcgatgactg gcgagatcac cttgcgcggc cgcgtgcttc cgatcggcgg cctgaaggaa
     5581 aagctcctag ccgcgcaccg ggcggaaatc gacgtcgtcc tagtccccaa ggaaaaccaa
     5641 aaggacctca aggagatccc gcgccgcgtt ctcaacgcgc tccggatcgt gctcgtcgaa
     5701 cacatggacg aagtcctgcg tgaggccctg gccctcagcg atcccgacgc cctctttggc
     5761 gaacatcagg tgcgccccgt cgagtatcgt gagggcgagc tgttcacgcc ggaggaagcc
     5821 gagaccgaag acgtcccgga gccgacggtg gacgcgcccg gggcccgtca gtagcggaaa
     5881 tgccccattg gacagcttgg gccgggggcg tcatgctcgc ggccactttg ggcggataac
     5941 tcagcggtag agtggggtct ttacacggcc taagcccccg gttcgaatcc gggtccgccc
     6001 accgagaccc caaaaaagca ggccctactc ctctttttgc tattggactg caggacttca
     6061 cgccctaggt ttgctaacta gtagtcagcg actgaccgtc ggtcagtcgc gctagagggc
     6121 aagcattggc ggtagcggca cgcagggaac gggagcgaac tgcacgtcga gaagcgattc
     6181 tcgacgcggc gcaggagctc atcgccgaag agggctatca cggtctacgc atggacgcgg
     6241 tcgcagaggc ggccgagctc agcaagggga cgctctacct ctacttcggg aacaaggacg
     6301 cgctttgcgc ggcggtcgcg atccggcttc tggatacgct gatcccggtg attacgtctg
     6361 cactcgagaa cacgcccacc ggtctcgacg cggtacggca gttgctccga agctactacg
     6421 attttactca gaagaacccg caccactttc gctttgccct ggcctggctc agcgcgggag
     6481 agcgtatgga cgattctacg gaggagttcc agagctatcg ggcccgtgtc ggacagatgc
     6541 tcatactcgt gacctccgcg ctgaagcggg ggcaggacga cggaagcatc cgggcggagg
     6601 tcgacccctt gccccaagcg cttcagttgt ggacgagctt cctcggcgtg gtgctcgtcg
     6661 gactcaacaa agaagcaatg gctcaaagaa taccggcttc cgtggatttg gatcagcttg
     6721 tgcccctaca tctcgacacc atgctcaggg cactttcgag cgaaggaaca tcatgatgcg
     6781 catcgcgatt cccgttcttc tggcctcggc gctgattgcc gggtgcaact acaagaagga
     6841 gaccgacatc ccacccccgt tggaggttcc ggagagcttc agcgagggct ccgaggtggc
     6901 gccgccagag ccggaagccg acgccctgcc ggagggcaag agcgggtccg agacgattcg
     6961 cgacgtgcgc tggtggcgtc agatgggcga ccccacgctc actgcgctga tcgaagaggc
     7021 gctgcggcgc aaccaaacgg ttcgcgccgg ctgggccagg gtccagcagt cccagtacat
     7081 cgccaacctg gttcgcgccg cgcgcatgcc gaccatcggt gccgtgggtg ccttcaatgc
     7141 ggcgaagaac atcacgccat tcgcggaaac gacttcgctc gacgcgcaag ggtcgatccc
     7201 cgtggcttac gaggtcgatc tcttcgcacg gcgtgcgcgc gagcaccagg ccgcgaagct
     7261 cgatgcggaa gctgcacggc tcgatcaaga ggccctggcg atcgcgattt ccgcatcggt
     7321 cgccgaagca tggttcgatt cggtcaacgc gcggatccaa aacgcagtcg tcgccgagca
     7381 gctcgaaacg gacctgaagt ttctcgagct ggtcgagctt cgctatcgtg agggactcaa
     7441 ctccgcggtc gacgtgcacc agcaacggca gcgcgttgcc ggtcaacgcg cgcttctggc
     7501 cgtggccgac tcgctgatgg agctgaccga tcagcggctt tcggtgctcc tgggcgaagc
     7561 gcccggccgc gagttcccag tggacggcaa gacactgcct gaaatcggtc cgacaccggc
     7621 catcgaagtc ccggcgtcac tgctagacgc ccgccccgac atccgggcca ttcagaaacg
     7681 cgtcgaagcg gaagatcgcc gggtcgcggc ggcaattggc gcgcgtctcc cccaagtcgt
     7741 gatcgatttc accccttcat acacctggct ccgatccaaa atcacctcca cgggcgcgtt
     7801 ggggggtgtc cctggtgaga cgaccgatcc gattaccgcc agtggcttca cctggaacac
     7861 aggggcgacc ttttcggttc cgctgttcga cgggattgcc ggcagatccc agatcgcagc
     7921 caatcgggct gccgtagacg cgctcctgga gacgtacgcc gaaacaatcc tgacggccct
     7981 cctcgaagtg gaaagctcgt tggtcctcga gcgtcaggaa cggctccgca ttcaattcct
     8041 cgaagaggaa ttcagcctgg ccaacatcac actcgaagcc acccgtgatc gctaccgtgc
     8101 tgggctgagt gacttcttgc ccgtgcttac ggcgctcgcg acccgccaga cctcggagct
     8161 tcagctcgtg gcctcacgaa gacaactg
//
LOCUS       DASRZF010000441         5899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3774174, whole genome shotgun sequence.
ACCESSION   DASRZF010000441 DASRZF010000000
VERSION     DASRZF010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 5899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5899
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3774174"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="VFG22_19375"
     CDS             complement(<1..888)
                     /locus_tag="VFG22_19375"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HET6418467.1"
                     /translation="MRTSACFAFLVSFMIGCGSPEEQFVGELTAPVVYGADDRVEVYN
                     HPNDKLRRIATESIVALIPSFRIDRQVDGNYALYTESLADLRGLCEDERFGNQPTAAA
                     CSGVLIDDDLVLTAGHCVDDHTPCDTYTYVFNYHLSDPNFLEAIRDEDVYSCARVVSQ
                     GNPSSGSFTPDFTVIQLDRPVEGQHAPVSIRPASPLAIQESLAMIGFGSGLPAKIDTG
                     GVVFDPRADSLDFFVAYLDAFQGHSGSATFDSENRLAGILIGGRTPDYVTLEGESCSR
                     VSVYQDSEAGEVVHNIAPIV"
     gene            complement(922..2355)
                     /gene="pyk"
                     /locus_tag="VFG22_19380"
     CDS             complement(922..2355)
                     /gene="pyk"
                     /locus_tag="VFG22_19380"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HET6418468.1"
                     /translation="MKLGIQPSQTRLTKIVCTIGPSTSSWDALAGLAQAGMNLARLNM
                     SHGDHVSHLQVIRNLKSLSKKLAHPVSILMDLQGPEIRTGELSKSLDLKSGEVFYFTV
                     LADDAEERSVHVNYLDLVKDLRVGERVTVDNGLINLEVLEVSEHRLKCRVLDGGTLGS
                     RKHVNLPGVRVNLPSITKKDREDITFAIEQDLDFIALSFVRGAEDVQEVREIVDAAGG
                     HQRLIAKIENQEGVEHFDSILEAADGIMVARGDLGVEVAFEELPVLQRDMIRRCAIAG
                     KPIIVATHLLESMIENPLPTRAEVTDVANAVYEQADAIMLSGETAAGKYPVRCVEVLD
                     TIARRIETVPGLDFYKERPAPTTQRAHIAQGACRLADSLGARAIVVMTRRGYFGQLVA
                     SYRPQRAIIYAFTNMSTTRRKLWLNRAVIPFRMNLSRDPEKTIVNALAKLRERNVIAS
                     GEPVVVVSDVATATGDLVTSIQVRVSE"
     gene            complement(2380..3453)
                     /locus_tag="VFG22_19385"
     CDS             complement(2380..3453)
                     /locus_tag="VFG22_19385"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HET6418469.1"
                     /translation="MKPPKAYRALFVTLLGMTLACGDPSNAADWGEEIGAASEKVVGG
                     WAATADQIFATVEIRHTGGASGFCSGTLISPTIVVTAAHCAVVEPEPGTLRPAQPASL
                     QVVAGHLVSRAAVPSMMRGVFKVIVHEDYDHEFIMGRSRSGAGQGGIGSPNDIALLFL
                     TAPFDGIPNAPLLSVQREAELVHHGDVAFVAGYGVYDLDDDLTGKLYISDAVIDIIGP
                     QELLTRRIDQLGDSCFGDSGGPLYVPTEEGDFLVGLVSRGRSDVKRDCGDGGVYTLLS
                     AYLDWIQETAGSRFEPSATSGPDADSFLEEGADPISVPKGGSLRRSAACAASSSSSTD
                     RSPISALALLLGFGLYRRRLSRV"
     gene            3607..4221
                     /locus_tag="VFG22_19390"
     CDS             3607..4221
                     /locus_tag="VFG22_19390"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6418470.1"
                     /translation="MERSSRRERRKEETLSRIETVAWDLFRRRGYEATSTREIAEGAD
                     IAAGTFFNYFPEKRSLLIHLMQRQIDSATNRAFETMAPSTLERELTELFAALTQCYAK
                     ERRLSRVFIKELLFTDGQRRTESAAWTFGLVKRISSLVERAQRRGELDPSIDPMEAAQ
                     QVFSTHYFGLVTWLGGTIPSRDAQEEQFRASLRLLLRGLRQSAA"
     gene            complement(4180..5115)
                     /locus_tag="VFG22_19395"
     CDS             complement(4180..5115)
                     /locus_tag="VFG22_19395"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6418471.1"
                     /translation="MEKLPAPDLPDWIAKDLPFTRYLLPVGDGLRMHVMESGRGRPVL
                     MVHGNPTWGYLYRKVAMCLQGQGLRLIMPDLIGFGLSDKPRDFSLHTIRNHSRWLGAL
                     IDQLDLHEVILVCQDWGGAIGTHPFTTRRERLGGMVVLNTVLDAPPPGFKPTAFHRFS
                     HTPLLSDFVFRVLPLPQAALHLAQGDKSSIRGKVALAYRYPFRNIRDRVAPLATARMA
                     VDRPDHPSIPALEECAALAGAFNGPKAMVWGRRDPVLARALKRTKALLGDPPVTETQA
                     GHFLQEEVPEEIAAAVIDVNARLSRALAQATEQQA"
     gene            5364..5693
                     /locus_tag="VFG22_19400"
     CDS             5364..5693
                     /locus_tag="VFG22_19400"
                     /inference="COORDINATES: protein motif:HMM:NF017451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HET6418472.1"
                     /translation="MKKNVYERAREVVRELVPVLQAIRDCDKSLADQMKRAAQSVVLN
                     IAEGRGSDAGNARARFSTACGSAKEVRAALHVASDWGYIESTTAARLDEKLDEVCAIT
                     WCLGRSR"
BASE COUNT         1154 a         1753 c         1900 g         1092 t
ORIGIN      
        1 aacgatgggc gcgatgttgt gcaccacttc gccggcctcc gagtcttggt agacggaaac
       61 gcggctgcag gactcgccct ccaaggtgac gtagtcaggc gtacgaccgc cgatgagaat
      121 gcccgctagg cggttctccg agtcgaaggt tgcgcttccc gagtggccct gaaacgcgtc
      181 caggtaggca acgaaaaagt ctaggctatc ggcgcgcggg tcgaacacga cgccgccggt
      241 gtcgatcttc gctggaagac cgctgccgaa gccgatcatg gcgaggctct cctggatcgc
      301 caaggggctc gccggtcgaa tcgaaacagg cgcgtgctgg ccctcgaccg ggcggtcgag
      361 ctggatgacc gtgaagtcgg gcgtgaagct ccccgacgaa gggttgcctt gcgaaacgac
      421 gcgggcacag ctgtaaacgt cttcatctcg tatcgcctcg aggaagttcg ggtcggagag
      481 atggtagttg aacacgtagg tgtaggtgtc gcagggcgtg tgatcgtcaa cgcagtggcc
      541 ggccgtgagg acgaggtcgt cgtcgatcaa gacgccggag catgccgcag ccgttggttg
      601 gttgccgaaa cgttcgtctt cgcagagccc tcgcaagtcc gccagtgatt cggtgtagag
      661 ggcgtagttg ccatcgacct gccggtcgat ccggaagctt ggtatcaggg cgacgatcga
      721 ttccgtggcg atccgccgaa gcttgtcgtt cgggtgattg tagacttcga cacgatcgtc
      781 ggctccgtag acgacgggcg cggtcagctc acccacgaat tgctcttcgg gggacccaca
      841 gccgatcatg aagctgacca gaaaagcgaa gcaggcagat gtacgcattg ataactgtct
      901 aaagccaaac cggcgcctcc gctactcgga gacccgaacc tgtatgctag tcaccaaatc
      961 tccggtcgct gttgccacgt ccgaaacgac cacgaccggc tctcccgatg cgatcacatt
     1021 gcgctcgcgc agcttggcca gggcattgac gatcgtcttt tcggggtcgc gcgagaggtt
     1081 catccggaac ggaatcaccg cccgattcaa ccagagctta cggcgggtag tgctcatgtt
     1141 ggtgaacgcg tagatgatcg cgcgctgagg tctgtagctt gcaacgagtt gaccgaagta
     1201 accgcggcgc gtcatgacga cgatggcccg cgcgcccagc gaatcagcca ggcggcatgc
     1261 gccctgcgcg atgtgagcgc gttgcgtggt cggggccggg cgctctttgt agaagtcgag
     1321 ccctggaacc gtctcgatgc gcctagcgat ggtgtccagc acttctacgc aacgcactgg
     1381 gtactttccg gcagcggtct caccgctcag catgatcgca tcggcctgct cgtagacggc
     1441 gttggcgacg tcggtgacct cggctcgggt cggcaacgga ttttcgatca tcgattcgag
     1501 cagatgggtt gccacgatga tcggttttcc ggcgattgcg cagcgacgaa tcatgtcgcg
     1561 ctgcagaact ggaagctctt cgaaggccac ttcgacgccg agatcccctc gggcaaccat
     1621 gatgccgtct gctgcttcga gaattgaatc gaagtgctcg actccttcct gattttcgat
     1681 cttggcgatg agacgctgat gcccgccagc ggcatcgacg atttcgcgaa cctcttgaac
     1741 atcttcggca cctcggacaa acgagagcgc gatgaagtcg aggtcttgct cgatcgcaaa
     1801 ggtgatgtct tcgcgatctt tcttggtgat cgacggcaga ttgacgcgta caccgggcaa
     1861 gttgacgtgc ttgcgagaac ccagcgttcc gccatcgagc accctgcact tgagccggtg
     1921 ctccgacact tcgagcactt ccaagttgat gaggccgttg tcgaccgtga cgcgctcgcc
     1981 gacgcgcagg tctttgacga ggtcgagata gttgacgtgg acgcttcgct cttccgcatc
     2041 gtcagccaag accgtgaaat agaacacctc gcccgacttc aggtcgagac tcttggaaag
     2101 ctcgccggtc ctgatctccg gaccttgcag gtccatgagg atcgagaccg gatgcgcgag
     2161 ctttttgctg agactcttta ggtttcggat gacttgaaga tggctgacgt ggtcgccgtg
     2221 agacatgttg agccgggcga ggttcatacc ggcctgggcc aaaccggcga gcgcatccca
     2281 cgagctcgtg gacggaccga tcgtgcagac gatcttcgtc agtcgcgttt gtgacggttg
     2341 tatccccagc ttcatcgctg cgtggagcct aggccaccgc tatacccgtg acaatcggcg
     2401 ccgatacaat ccgaagccca gcaagagagc gagagctgaa atgggcgatc ggtcggtcga
     2461 gctcgaggaa gacgcggcgc atgcggcaga tcgccggagg ctgccaccct tgggcacgct
     2521 gatcgggtcg gcgccctcct ccaggaacga atccgcgtcg ggaccgctcg ttgcgctagg
     2581 ctcgaatcgg ctccctgcgg tctcttggat ccagtcgaga tacgcagaga gcaaagtata
     2641 gacgccgccg tctccacaat cacgcttcac gtcggatcga ccccgcgata cgaggccaac
     2701 caggaagtcg ccctcttcgg tgggaacgta gagcggaccg ccggagtcgc cgaaacagga
     2761 gtccccgagt tgatcgattc gtcgcgtgag cagctcttgt ggaccgatga tgtcgatgac
     2821 cgcgtcggag atgtagagct tgccagtcag atcgtcatct aggtcgtaca cgccgtagcc
     2881 cgctacgaat gcaacatccc cgtggtgcac gagctcggcc tcgcgctgga cgctcagcaa
     2941 gggagcattc ggaatcccat cgaaaggcgc cgtcaagaag agcaaagcga tgtcattggg
     3001 cgagccgatg cccccttggc cggcgccgct acggcttcga cccatgatga actcgtggtc
     3061 gtagtcctcg tgcacgatca ctttgaagac gccgcgcatc atgctcggga cagcggctcg
     3121 gctgacgaga tgtccggcga cgacttgcag cgaagcgggc tgggctggtc gtagagtgcc
     3181 cggctcgggc tcgaccacag cgcagtgcgc agcggtcacg acgatcgtgg gcgagatcaa
     3241 ggtacccgag cagaagcccg aagcaccacc ggtgtgccgg atctcgacgg tggcgaagat
     3301 ctgatcggcc gttgcggccc aaccgcctac gaccttctcg ctcgcagcac cgatctcttc
     3361 gccccaatcc gcagcgttgg acggatcacc gcaggcaagg gtcatgccca aaagggttac
     3421 aaatagggct ctgtatgcct ttgggggttt catggggcaa ggtaggtgtc agtatcatgg
     3481 tcgaatcaag cttcatagag tgaacgcttg tcatcccctc tcgatccgct gattcggtga
     3541 gctgacgaac ctttgccaag gtgcggaaaa cttggtggcc gacgcgcgca gaacgacagt
     3601 cggcgagtgg aacgctcgag tcgacgcgaa cgccgcaaag aagaaaccct gagccgcatc
     3661 gaaacggtgg cctgggacct gtttcggcgt cgaggctacg aggctacgtc gacccgcgag
     3721 atcgcggagg gggctgacat cgccgcgggc accttcttca actacttccc ggaaaagcga
     3781 agcctattga tccacttgat gcagcgtcag atcgactctg caacgaatcg ggcctttgaa
     3841 acaatggcgc cctcaacgct cgagcgagag ctcaccgagc tctttgccgc cctcacgcaa
     3901 tgctatgcga aagagcgacg cttgagccgt gtcttcatca aggagctgtt gttcaccgat
     3961 ggacaacggc gcacggaatc cgcggcgtgg acattcggcc tcgtgaaacg catctcgagc
     4021 ttggtggaac gggcgcaacg tcgcggggag ctcgatccct cgatcgaccc gatggaagcc
     4081 gctcagcagg tgttcagcac gcactacttc ggcctcgtca cctggctcgg aggcacgatt
     4141 ccgtcacggg atgcgcaaga ggaacagttc cgcgcgtcgc tacgcctgct gctccgtggc
     4201 ttgcgccaaa gcgcggctta gcctcgcgtt gacgtctatg accgcagcgg cgatctcctc
     4261 gggcacctcc tcctgtagga agtgaccggc ctgggtctcg gtgacaggcg gatcaccgag
     4321 caaggccttg gtgcgtttca acgcacgcgc gagaaccgga tcgcgccggc cccagaccat
     4381 ggccttcggc ccattgaagg cgcccgccaa ggctgcgcac tcctcgagcg caggaatcga
     4441 agggtgatct gggcgatcca ccgccatacg ggccgtcgcc aagggtgcga cgcgatcgcg
     4501 gatgtttcgg aacgggtagc ggtaggcgag cgcgaccttg ccgcggatgc tggacttgtc
     4561 gccctgggcc aggtgcaggg ctgcctgcgg caagggaagg acgcggaaca cgaagtcgct
     4621 gagcaaggga gtgtgggaga accgatggaa tgcggttggc ttgaagccgg gcgggggcgc
     4681 gtcgagaacc gtgttcaaga cgaccatccc accaaggcgt tctctgcgag tcgtgaaagg
     4741 atgcgtgccg atcgcgccgc cccagtcctg gcagaccagg atcacttcat ggagatcgag
     4801 ctgatcgatc agcgccccga gccagcgact gtgatttcga atcgtgtgaa gcgagaagtc
     4861 cctcggctta tcgctgaggc cgaaaccgat caggtcgggc atgatcagcc gaagcccttg
     4921 cccttgcaga cacatcgcga ccttccggta gaggtatccc caggtgggat ttccatgcac
     4981 catcaagacc ggtcgtccgc gaccgctttc catcacgtgc atccgcaacc catcacccac
     5041 gggaagcaag tagcgggtaa agggaagatc cttcgcgatc cagtcgggaa ggtcgggcgc
     5101 aggcagcttt tccacgcgcg gagcgtaccg cctccagcgg agacacgaaa gccggcagaa
     5161 atcacgctgg acgctggacg ctggcgcggg cactgacact ggcgccggca ctggcactgg
     5221 cgctgacact ggcgctgaca ctggcactgg cactggcact ggcactggcg ctggcgcttc
     5281 tggagctctc cttgtcatcg cttgtccttg gattgggttc tggccgcacg ggtgaggcca
     5341 gaacccaatc caaggagtcg ctaatgaaga agaatgtgta cgagagggcg cgagaggtcg
     5401 tgcgggagct cgttcccgtg ctgcaggcga ttcgcgattg tgacaagtcg ctcgccgatc
     5461 aaatgaagcg ggctgcccag agtgtggtgc tgaatattgc agaaggacgg ggtagtgatg
     5521 cggggaatgc gcgggctcgg ttctctaccg cttgtgggtc agccaaagag gtccgggcgg
     5581 cgctccacgt cgcgagcgac tggggctaca tcgagtcgac gacggcggca cggctcgacg
     5641 agaagctcga cgaggtctgc gcgatcactt ggtgcctggg ccgcagccgc tagcggctcg
     5701 agagccgcca cgacatcgcg cagacttcgt cgagtgcggt gtcaatggct tggacctacg
     5761 cgccggggaa gtatccgtag gcgacgccga gctggagtcc ggcgcgcact tccttggcgg
     5821 agccacatgc cgacgcgaag cgcgctctcg ccgtgccgag atcgtttccg tcagcttcgg
     5881 cgatgttgag cacgacact
//
LOCUS       DASRZF010000442        16209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_339041, whole genome shotgun sequence.
ACCESSION   DASRZF010000442 DASRZF010000000
VERSION     DASRZF010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 16209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16209
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339041"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="VFG22_19405"
     CDS             <1..665
                     /locus_tag="VFG22_19405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418473.1"
                     /translation="SRWLWDGEYWGFQDAGWAAEGSARVLYRPTALPGGSASTVPVPD
                     SQQSEVELSVAPSSYSVYVWTGTWVAPPIVYPTAGSTGKDSIRYTRRYKQNMPSSTST
                     SELAAPVPDRLINVDSPPAVAKTHEAEREDEVELIPGVLHGGARGEEELRLMEEAKQR
                     EAQEQEDGVDSVYVPYYYDYYYGRYPARPRPPRPVRPDPSRPTPLPAPAPPSRRPRPP
                     IR"
     gene            908..4522
                     /locus_tag="VFG22_19410"
     CDS             908..4522
                     /locus_tag="VFG22_19410"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HET6418474.1"
                     /translation="MAIAQDDVERPDEPTHERSLSFLGRLRRLCLPVLLGLVGTCSLL
                     VVAGLWILANLDHRWVKSKVQHLLSDLVGTNVSYEQLSLSPFSGLQLHGLVLATPEAL
                     RSHAPEMLRLEELLVPIELGSLFSGDVVIPEIRGGSVAVTVVIADDGRNSFGELVQSE
                     EEPEQSSTPLSQSLDGLEHLSLSVGSIRLAPIRFRAVEVSAGAVTRQTDLESLGISSD
                     GISIGRESRGSISLAPHGRDDVVLSVLERAQTASTHAPPLRQAEVAPKVELRLESSRA
                     LALTASAELHEQSLFAELRPVRSLVRLESSVSFDPDDSRTNVHVEHLALLDSMLVATA
                     DAVLHDSSTARLDGHGELHAASLPWALPWLSIDDLTGRFEIQGLEIEPAGVSAGTATL
                     QGQLASARYAEGPASLELHGAAISGVISAPEDPALKAGKLTVEASVQKAVAKERGRFA
                     ATIGKLATTLELRGLGTEDSGMWGLQGTGSLDGSMDRASVKVKNVSAASRATVALDVD
                     LANHRIAAKLPIESFTLARVGHDRITIRGAQIDLVAHEPLNWTTEAGAPAIELKASVD
                     RVSVGKRHFRARKSSFEARRTEADRYAVAALLNADRVSWGNFRSEPESSLSFEAALDT
                     KRPALDATAALSIAGDATTELSLSASHDSPITRYSLDLHGAEAGPLLGAILFGDGGLR
                     SDELSFAFKSEGKFRGLVAKDRAGTPVLSKEPLRNTRGAHQSELRVERLVLVRAGITH
                     QLRGLTVQARSTHEAPGYGSLKASASIEEARYGEVAEPVQLLDYEQQLQVAYAALHGA
                     PSFSIETNGTLGQLDQPFVRQYPVRDVSFGASVDVDDTQVFAVRHAYWRNPAGGTSLE
                     ARGAYEGWQDAIRNTEVCTAGMAGCPEVASMYGREAATVTGTFEQDFGFWESTDSVKS
                     SGSLVVPFTIESGDLNTYRIVATAQFRDVVLELPQYGFVVEDLDALIPMEQEFATAPK
                     LFIVPSRTANAITQKRFFDLHPFTKRESFFTVDRVQLGQEVIGPVAANLQVVGSVIAM
                     DQLNAAYRDGFVTGQFLADLNRKDPKVVFRGNLTGVKTTHGRGVLDANLAMTFVPTTL
                     ILEGKAQIVRVSKDHLYEIIDVLDPYHEDEDMNRVRLGLKFGYPKFVLLKFNDGLMDA
                     KVDLGGLAGAIRIDEIKGIPVTPFMEQYVQPYIERISSPSLIYDVTPTETMSSEADGT
                     "
     gene            4519..5085
                     /locus_tag="VFG22_19415"
     CDS             4519..5085
                     /locus_tag="VFG22_19415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418475.1"
                     /translation="MISFGSNLSRFAALVVLASGAGCIKAPPIAVVDNRTAIEVQASG
                     EYPDLEFQSADATLQPGPSPVSGKDIVSKAGLSAAGADLDLFELSRSDAQLIDAMLLI
                     GCLGEGEDGLLKYTPDRCDQNVEVSELLRVAGRNNLHRRQVWEYLASRRPDQSAVLAR
                     DAWRSVHLEQVRCGAWIEKDGAWAQKTC"
     gene            5064..8648
                     /locus_tag="VFG22_19420"
     CDS             5064..8648
                     /locus_tag="VFG22_19420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418476.1"
                     /translation="MGAEDLLRGARALSALVTAVALALPSPALANGRDGTVNQEEERR
                     QTPALLKPRRLTVGDQNHFMGVLGPGGRYLYYVTDEYKSYDIFIQSPIDSAGQPLFEA
                     FGDVVWPTISPDGRELAYIRYETEAQGDACRRRIRNDGSARNKREDCHATDEADLQIH
                     WRNDGRLGVLLREELHGNLVLLDGAFEKDPQRKEANVVGLALSHDERWVAYVPLVRRH
                     DEIGVSFSNQIGTEGIRIRRTREGAREFGYEPKLPGVSGYPVFSPDDAYLYFSQFLND
                     TNEDGVIDGDDNGVLFRVPFRGDAEKPEFGQPRQLTNARWNCHYPSVRDTSMALTCAI
                     GRNLHIYLLPASGAVPADWNQERIAAETESVRDAWSELLLRQHLLVGARDDLERSEQL
                     RALTKLHLDLREYEAAIYYGEQRKELLRTSGVTDRWSELMILLAKHQRADKALSRGRL
                     SEEYVQESITLAEQVNSVDTDQSPDSAALQSLVLSKIESDSGDKAAAERLLDRVNLAP
                     VQDETVIDLAFQQFDHFYGLLADYEKRLARLKEVALHPAPSVTESLRHCKSFVDLLQR
                     GQPRDARVARLIDARRTVPDQSHLALALDLEIALDQLTEDNAKAVEEQLVGLFSATKN
                     AHVRRYLVVSALRAAHRRGVPSLEQALLERWVASIEQDDPERKTSTALYELVVLERAY
                     AAFDKGDMETAARYFDKARGATDSLAGHVGWIETELRLGSKNPQALDQRKGGKKHARH
                     AVSESEATRQFVEAYLLARDLGSIESNKDLERTVERAQTLLEAAIEEDPNQPLEHLLL
                     AHALHHRGMRLGRRDDMALAVRHYLLALDLAQKQPRVRAPTHAGMALAQASLGNHRRA
                     LEDYEARLDLPTISAEEEIGLLVEYARSLFHVNQQPKAIEALRGALALMHQDGEVAEV
                     SGATGHPTEGLERYEPLILDRLALYELDARQTEHSMETHAQLQRSLQSHPEADSAVNE
                     VKTWARLAAAHLAGGDPKSALGFATKGQERLSSADPLRPKDIDRRIRPVTHEFVYDEE
                     QLRILLAGLEASAARQTGDWEQARASLLIRKEALEERYERQEVDEDALELALTCLRLA
                     EVAQKRRDLQQTKRYLEEGLAYTDRHAGSTGSDVSEVGFHLLRAYAELHFSGGVDLAS
                     YQRDLEQDLLRYYVFLSEVRNPQWETERIRFEIYLSLLRMEKAPAPEP"
     gene            complement(8659..9483)
                     /locus_tag="VFG22_19425"
     CDS             complement(8659..9483)
                     /locus_tag="VFG22_19425"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HET6418477.1"
                     /translation="MQNARTVGAVFLAAIMGCQSANMNDTPKDNPITPTPGAGALGEL
                     QVDVFYRERMLLPPTAELKVTFEDSAKMDVAAEKIAEATVPIQGAPPYRVTLSYDPSK
                     LDARGRYGVRARIENEGQLMFTSTQMNPAFGVDGSKDGAPNDPVKVLVSRVAAARKPN
                     APTLTDTRWMLVKLRTEGATLGAGGKAPSLTLMADEQRISGFAGCNQMIGGYQLDGDK
                     LSFSKIAMTMRACPEGMDLEREVGRALEETRSYEISADVLRLHSEDGTIVAEFKAD"
     gene            9642..11405
                     /locus_tag="VFG22_19430"
     CDS             9642..11405
                     /locus_tag="VFG22_19430"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HET6418478.1"
                     /translation="MSSPLRALILLASLSALSGAAGCNRSPKAPASPSVDAADTIYVG
                     GDVVTIDDAQPTAEALAVKDGRIVAVGARADVERAHKGATTRIVDLGGKTLMPSFIDS
                     HSHYYNSLLVANQAKLYAPPSGPGKDVPSIVAELKKFAAERKIPKGEMIMGYGYDDTV
                     MPDGRLLNRDDLDAAFPDNPVRIDHVSMHGAVMNSLALKKYGFSAKTKTPPGGVIVRK
                     PGSKEPYGLIMEMAFLPVIERQEPMTPEQEIELTKAGQMLYAEAGLTTAHEGATHLAQ
                     LQTIKRASEAGANIIDVVAFPFITDVDKTLKEFPLESWGKYDQRLKIGGVKISIDGSP
                     QGRTAYFTTPYLTGGPGGEKNWTGDLLASQEVINQALKKVYDLDVPVLFHANGDGAID
                     SLITAHEFAAAGDPTRYRNVTAIHAQFTRKDQIPKFVEYKIRPSFYTLHTYYFAEAHI
                     ANRGLKQAMYISPMHDAIDAGLHPTNHTDFVVAPLDQMMMLWTAVNRISRAGAEIGLD
                     QRVTPLEGLKAMTIWAAEQYGEEGSKGSLAPGKLADLVILDRNPLEVDHMTIKDIKVV
                     ETIKEGKTIYARSSDAECCAR"
     gene            complement(11427..12008)
                     /locus_tag="VFG22_19435"
     CDS             complement(11427..12008)
                     /locus_tag="VFG22_19435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418479.1"
                     /translation="MKHIEKQSSTARFGTGAVIAAAFALISVTACGSPVRQVRTAQAE
                     AVATSSSDEGIPAGTTIPVVALLQIESRNADSEQPSTTGQQPYFAVVQDITNSEGTVL
                     VHEGARVMASVVRRKNPRIGRPGWMEVSFKSTTGANGTVVRLDDAPQRFEGKSRIKGS
                     ITLAVLTYGLGLLRTGGDVTLPEGTGLVARVVD"
     gene            12107..12970
                     /locus_tag="VFG22_19440"
     CDS             12107..12970
                     /locus_tag="VFG22_19440"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET6418480.1"
                     /translation="MERWDDLRIFLAAFREGSCAAAGARLGVNQSTISRRISALEREL
                     GVRLFDRTPEGLVPTAAAEEIVPRAELFEVTAAELIGAIEGLDTRPGGVVRIASPVLI
                     ASELLAPALPAFLDEQPGLRLELIVGDAIVDLSRREADLALRFVRPDRGDLLVRRVGT
                     LRFAVFGSKSYLDMHRGQRAEALAWLDWDTTQAQLPDAAWLRAEFPGIEPVLRTNNLG
                     VRLRATRAGLGVSVLARSLAARYSELEALEDLPVIPDIPVWLVGHRALRNLARIKAVW
                     AFLEELSAELT"
     gene            complement(13026..14489)
                     /locus_tag="VFG22_19445"
     CDS             complement(13026..14489)
                     /locus_tag="VFG22_19445"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HET6418481.1"
                     /translation="MSQTVPNDRIRDGPKHQHQENEMTLHDKESIREQLDDEIYASSE
                     LLVGMPKYRFPKSESLAKAVYAAVHDELMLDGNSRQNLATFCQTWAEPEMHKLLDECM
                     DKNIVDKDEYPQTAEIEARCVHMLADLWNSPEAASTIGCSTTGSSEAAMLGGMAMKRR
                     WENTRKAAGKSIEKPNLVTGPVQVCWHKFTRYWDIEHREIPMEHGRLLMTPEEVIKRC
                     DENTIGVVPTLGVTFTGQYEPVQAVAKALDEYEKKTGLDIPLHVDGASGGFLAPFCAP
                     DLVWDFRLPRVKSINASGHKFGLSPLGVGWIIWRDEHDLPDELVFWVNYLGGNMRDIG
                     LNFSRPGGQVVCQYYNFLRLGQQDYRRVHEACYDTAQYIAGEIEKLGPFEIIYPGDMR
                     SGIPALCWKIKDGVDPGFSLYDFADRLRSRGWQVPAYTLPPNCEDLAIQRILVRHGVS
                     HDLGSLLLDDMKRALDYFARHPVQSRLTKEEASGFHH"
     gene            complement(14563..>16209)
                     /locus_tag="VFG22_19450"
     CDS             complement(14563..>16209)
                     /locus_tag="VFG22_19450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017504.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6418482.1"
                     /translation="PYNPQTAEVEFEPAPLFGQVGQAVEQLRELGYRAHYGRWLVSGR
                     PHVVLLDVGSVGAMVNEIKYRVWADHGIPASDEPGVSDAVAFGEMCRALLSVLAQEDS
                     QRRRVVAHFHEWQAGLAIPMLRKERWPGSLVFTTHATLVGRYLATGNTSFYDQLPSFD
                     GDQEAKNFNIDAQHQIEKAAAHASHVFTTVSDLTAEECTHLLGRTPDMLLPNGLNIRR
                     FTATHEFQTLHQQHKERLHRFSMGHFFPSYNFDLDNTLYMFTSGRYEYLNKGMDLTIE
                     ALARLNHRLRETGSPVTVVFFIITKAPFHSINVRALQSRAMLREFRTASDAVKESVGD
                     GLFRAASTGEIPDLNILMDDYWRLRLRRAIHAWKSEALPGIVTHDLIDDAHDPVLNQL
                     RTCRLFNQQDDPVKVVFHPDFVQATSPLFGMEYEQFVRGCHLGVFPSFYEPWGYTPLE
                     AIALGVPAVTSDLSGFGSYLKQQLPDHASKGIYVVPRRHNDFHRAADELTDRLFRFAS
                     LSRRERVILRNSVEAFSEHFDWHNLGRHYDEAHDLALERH"
BASE COUNT         3153 a         4922 c         5004 g         3130 t
ORIGIN      
        1 catcacgatg gctctgggat ggcgagtatt gggggtttca ggatgccggc tgggcggcgg
       61 aaggcagtgc gcgcgttctc tatcgcccaa ccgctctacc cggtgggagc gcaagcaccg
      121 ttccggtgcc cgattcgcag cagtccgaag tcgagctgtc cgtcgctccg tcctcgtact
      181 cggtgtacgt gtggaccggg acctgggtcg cgcctcccat cgtgtacccg accgcaggca
      241 gcaccggcaa agattcgatt cgctacacgc gtcgctacaa acagaacatg ccgagctcca
      301 cgtccacgtc tgagctggca gcgccagtcc ccgatcgtct catcaacgtc gactctccac
      361 cggcggtcgc caagacacac gaggccgaac gggaagacga ggtggaactg attcccggtg
      421 tgctgcacgg cggcgcacgc ggcgaagaag agcttcgttt gatggaggag gcgaagcagc
      481 gcgaggcaca agaacaagaa gacggcgtcg actcagtcta cgtcccttac tactacgact
      541 actattacgg gcgctacccc gcgcgtcctc gaccaccgag gcccgtgcgg ccagacccgt
      601 cccgtccgac accgctgccg gcccctgctc caccatcgcg gcgtccgagg ccgccaattc
      661 gttagcgcaa cgtcgtgatg ctagtcagtc ggcgtcatcg cgatcttgca tgacgctttc
      721 agacgacagg ctccgcccga attcttcaag actcacgcga gcgcgccgaa ccgcgtggcg
      781 gccatcgacg aggtctaccc aaacggtgtg ccggctgggc tgaagcccta ggcatgggcg
      841 cccgccaggc gctgttcgcg cgactcgctg gcgatgccct gttttttgcg tcaggtttcc
      901 gcgcctaatg gcgatcgcac aagacgatgt ggaaaggccc gacgagccaa cgcacgagcg
      961 gtcgctatcc ttcctaggtc ggctgagacg gctctgtctt cctgtgctcc tgggcttggt
     1021 gggcacatgc tctcttctcg tcgtggccgg tctttggatt ctggccaacc tcgaccatcg
     1081 atgggtgaaa agcaaagtcc agcacttgct ttctgatctc gtgggcacga acgtcagcta
     1141 cgagcaattg tcgttgtccc cgttctccgg tttgcagctg cacggcttgg tgctggcgac
     1201 tccggaggcg ctgcggtcgc acgcgccaga gatgctgcgc ctcgaggagc ttctcgtgcc
     1261 gatcgagctt ggctcgctct tttcgggaga cgtggtgatt ccagagatac gcggcggctc
     1321 ggtggcggtc acggtcgtga tcgcggacga cgggcgcaac tccttcggcg agctcgttca
     1381 gtcggaagag gagccggagc aatcgtcgac tcccctatcc cagagtctcg acggactgga
     1441 gcatctgtcc ttgagcgtgg gctccattcg actcgcgccg attcgctttc gggcggtcga
     1501 agtgagcgcc ggcgccgtta cacgtcaaac cgatctagag tccctgggca tctcttcgga
     1561 cgggatttct atcggaagag agtcccgggg ttctatatct ctggccccgc acggacgcga
     1621 cgatgtcgtt ttgtcggtgc tggagcgcgc gcagacagcc tcaacgcacg cacctccact
     1681 tcgtcaagca gaggtcgcgc cgaaggtgga gcttcgacta gaaagcagtc gtgcccttgc
     1741 gttgaccgcc agcgcggagc tccacgagca gtcacttttc gccgagcttc gtccggttcg
     1801 ttcgctagtc cgcctcgagt cgagcgtgag tttcgatccc gacgattcgc gcacgaatgt
     1861 gcacgtcgaa catcttgcgc tgctcgactc catgctcgtg gccacggcag atgcggtgtt
     1921 gcacgactcg tccaccgctc gcctcgatgg tcacggcgag ctccatgcgg cctcgctgcc
     1981 atgggcgctg ccgtggctga gcatcgacga cttaactggc cgattcgaga tccagggcct
     2041 cgaaatcgag cccgccgggg tttctgcggg gactgcgacc ctccaaggtc aactcgcaag
     2101 cgcccgttat gccgagggtc ccgcgtctct tgagctccac ggcgccgcga taagtggcgt
     2161 gatctccgct cccgaggatc cagccctgaa agccggaaag ctgaccgtcg aagcctccgt
     2221 ccaaaaggcg gtggcaaagg agcgcggtcg ctttgccgcg acgatcggca agctcgccac
     2281 cactctggag cttcggggcc tgggtaccga agactcgggg atgtggggct tgcaagggac
     2341 cggttcgctc gacggttcga tggatcgggc atccgtgaag gtgaagaacg tgtcggccgc
     2401 gtcccgggcg acggttgcgc tcgatgtgga tctcgcaaac catcgcatcg ctgcgaagct
     2461 cccgatcgaa tccttcacgc tcgctcgcgt tgggcacgat cggatcacga tacgtggcgc
     2521 acagatcgac ctggtcgctc acgagccgct caactggacg acggaggccg gtgctccggc
     2581 catcgagctg aaggcgtcgg tcgaccgcgt gtccgtcggc aagaggcatt ttcgtgctcg
     2641 aaaatcgtct ttcgaagcga ggcgaaccga agccgatcga tatgcggtcg cggcgttgct
     2701 caacgcagac cgggtgagtt ggggtaattt tcgtagtgag ccggagagct cgctttcgtt
     2761 cgaggcagcg ctcgatacga aaaggccggc gctcgacgcc accgcggccc tttccatcgc
     2821 tggggacgcc accacggagc tctcgttgag cgcttctcat gattcgccga tcacccgtta
     2881 tagtttagac cttcatggcg cggaggccgg gcctctcctc ggagcgatcc ttttcggcga
     2941 cggtggactg cgctcggatg agctcagctt cgccttcaag agcgaaggca agttcagggg
     3001 cctcgtggcg aaggaccgcg ccggcacccc cgttctatcc aaggagccgc ttcgaaatac
     3061 ccgaggtgcg caccagtccg agctgcgagt ggagcggctg gtgctagtgc gggcaggtat
     3121 cacgcatcag ctccgcggtc tgaccgtcca agctcgctct acgcatgagg ctccgggata
     3181 cggctctctg aaggcgagcg cgtcgataga agaagctcgc tacggcgaag tcgcggagcc
     3241 cgtccagctg cttgactacg agcagcagct gcaggtcgct tatgcagcgc tccacggcgc
     3301 gccctccttc tcgatcgaaa cgaacggcac cctcggccag ctcgatcagc cgttcgtgcg
     3361 ccagtacccg gttcgcgacg tatcctttgg agcaagcgtc gatgtggacg atacgcaagt
     3421 ctttgcagtc cgtcatgcat attggcgaaa ccccgccgga ggcaccagtc ttgaagcgcg
     3481 cggtgcgtac gaaggctggc aggacgcgat ccgcaacacc gaggtctgca ctgcgggcat
     3541 ggctgggtgt ccggaggttg cgtccatgta cggtcgagaa gcggccactg ttaccggcac
     3601 cttcgagcaa gacttcggct tctgggagag caccgacagc gtcaagagca gcggatctct
     3661 agtggtgccg tttaccatcg agtcgggaga cctcaacact taccgcatcg tggcaacggc
     3721 gcagtttcgc gacgtggtgc tcgagctccc gcagtacggg ttcgtcgtcg aagatctcga
     3781 tgcgctgatt cccatggagc aggagttcgc caccgcacca aagcttttca tcgtgccctc
     3841 gagaacagcg aacgcgataa cgcagaagcg cttcttcgac cttcatccat tcacgaaaag
     3901 agagagcttt ttcacggtag accgggtcca actcggtcag gaggtcatcg gccccgtggc
     3961 cgcgaacctc caagtcgtgg ggtccgtcat agccatggat cagctcaacg ccgcataccg
     4021 agacggtttt gtcacaggcc agtttcttgc ggatctcaat cggaaggatc ccaaggtcgt
     4081 ttttcgtggc aacctcaccg gcgtgaaaac gacgcatggg cgtggcgtcc tagatgcaaa
     4141 cctggccatg accttcgtgc cgacgacgct catcttggaa ggaaaggccc agatcgtacg
     4201 ggtgagcaag gatcatctgt acgagatcat cgatgtgttg gacccttacc acgaagacga
     4261 ggacatgaat cgagtgcgtc taggcctgaa attcggatat ccgaagttcg tgctcctcaa
     4321 gttcaatgat ggcctgatgg acgccaaggt cgatctcggt ggtttggctg gggcgatacg
     4381 catcgatgag atcaagggga tcccggtgac gcccttcatg gagcaatatg ttcaaccgta
     4441 catagagcgc atctcgtctc cttcgttgat ctacgatgtg acgcccacgg aaacgatgag
     4501 ctcggaggca gacggaacat gatttcattc ggtagcaatc tttcgcgttt tgcggctctg
     4561 gtcgtcttgg cctccggtgc gggctgcatc aaagctcctc ccattgcggt tgtggacaat
     4621 cgcaccgcaa tcgaagtgca agcatccggc gagtaccccg atctcgaatt tcaaagcgcc
     4681 gatgcgacgc ttcagccagg accaagcccc gtttccggca aagatatcgt ctccaaggcg
     4741 ggcctctcgg cggcaggtgc tgatctcgac ctcttcgagc tctcgcggag cgatgcgcag
     4801 ctcatcgatg cgatgctcct gatcggctgc cttggagaag gggaggatgg cctgctgaaa
     4861 tatacgccgg atcgttgtga ccagaacgtc gaggtgagtg agctcttgcg cgtggccggc
     4921 cgcaacaatc tccatcgacg acaggtctgg gagtatctgg cttccaggcg acctgatcag
     4981 tctgcggttt tggcgcgaga tgcatggcgc tcggtgcacc tcgagcaagt gcgttgtggc
     5041 gcatggatcg agaaggatgg agcatgggcg cagaagacct gctgagggga gcgcgtgctt
     5101 tgagcgctct ggtgactgcc gtcgccttag cgctgccaag ccctgcactt gcaaacggac
     5161 gcgacggcac cgtcaatcaa gaggaggaac ggcggcagac gcccgccctg ctcaagccga
     5221 gaagacttac cgttggcgac cagaatcact tcatgggcgt tctaggacct ggcggccgct
     5281 acctctatta cgtcacggac gaatacaagt cttacgatat cttcatccag tctcccatcg
     5341 attcagccgg gcaaccgctt ttcgaagcct tcggggacgt cgtctggccg acaatcagcc
     5401 cagacggaag ggagctcgcc tacatacggt acgaaaccga agcgcagggc gatgcgtgcc
     5461 ggcggcggat ccgaaacgac gggagcgctc gcaacaagcg ggaagactgt catgccaccg
     5521 acgaagcgga cttgcagatt cactggcgca acgacggccg cctaggagtt cttctgaggg
     5581 aagaattgca cggaaacctc gttctcctgg acggagcatt cgagaaggat ccgcagcgaa
     5641 aagaagcgaa cgtcgtggga ctggcgctgt ctcacgatga gcgctgggtt gcctacgttc
     5701 ccctcgtgcg ccggcacgac gaaatcggcg tctctttttc gaaccaaatc ggcactgagg
     5761 gcattcgcat tcgtcggacc agagagggcg cgcgggagtt tggatacgag ccgaagctcc
     5821 cgggagtgtc gggctatccg gtgttctcgc ccgatgacgc gtatctctat ttctcgcagt
     5881 tcctcaatga tacgaacgaa gacggtgtca tcgacggcga tgacaacggt gtcctgttcc
     5941 gggtgccatt ccgtggcgat gccgagaagc cggagtttgg tcaacctcga caactcacca
     6001 atgctcgatg gaactgccac tatccgtcgg tacgagacac gtccatggcg cttacctgcg
     6061 ccatcggtcg gaacctgcac atctacctct tgccggcgag cggggccgtg ccggcagact
     6121 ggaaccaaga gcgaatcgcc gcagagacgg aatcggtgcg cgacgcgtgg agcgagcttc
     6181 tgctccggca acatctgctc gtcggcgcca gagacgacct cgagcggtcc gagcaactaa
     6241 gggctctgac gaaattgcat ctggatctgc gcgagtacga ggccgctatc tattacggag
     6301 agcaacgcaa ggagcttctt cgcacctctg gtgtcacgga ccggtggtca gagctgatga
     6361 tccttctggc gaagcaccag cgggcggaca aagcgctgtc gcgaggccgt ttgagcgagg
     6421 aatacgttca ggagtcgatc acgctcgctg agcaagttaa ctccgtcgat actgatcagt
     6481 ctccggactc ggctgcgctt caatccttgg tgctcagcaa gattgaaagt gattcgggag
     6541 acaaggcggc cgcagaacgg cttcttgatc gggtgaacct cgctcccgtt caggacgaga
     6601 cggtcatcga cttggcgttt cagcagtttg atcacttcta cggcctgctg gcggactacg
     6661 agaagcggct ggcgcggttg aaggaggtgg cgctgcaccc cgctccttcg gttacggagt
     6721 cgctccgtca ctgcaaaagc tttgtcgacc tgcttcaacg tgggcaaccg cgagacgcga
     6781 gggtcgctcg cttgatcgac gctcgacgaa cggttccgga ccaaagccac ctcgcactcg
     6841 ccctcgatct ggagatagcc ctggatcagc tcacggaaga taatgcgaag gccgtcgagg
     6901 aacagctcgt tggtctcttt agcgcgacga aaaacgccca cgtacgccgg tacctcgtgg
     6961 tcagtgcgtt gagggcagcg cacaggcgcg gagttccatc tttggagcag gcgctgctgg
     7021 agagatgggt agcgtcgatc gagcaagacg acccggagcg aaaaacctcg acggcgctct
     7081 acgagctcgt ggtgctggag cgagcctatg cagcctttga caagggagac atggaaaccg
     7141 cagcacgcta cttcgacaag gcccgtggag ctacagattc acttgcaggc cacgtagggt
     7201 ggatcgaaac ggagttgcgg ttgggttcca agaatccaca ggctctcgac cagaggaagg
     7261 gcggcaagaa acacgcccgc catgcggtct ctgaaagcga ggcgacgcgg caattcgtcg
     7321 aggcctacct gttggcacgg gatctaggat ccatcgagag caacaaagat ctcgagcgca
     7381 ccgtggagcg agcgcagacg ctgctcgaag ctgcgatcga agaggacccg aatcagcccc
     7441 tggaacacct gttacttgcg catgcgctgc atcatcgagg gatgcgccta ggtcgccgtg
     7501 acgatatggc gctagcggtt cggcactatc tattggcctt ggacctcgcc cagaagcaac
     7561 ctcgcgttcg tgcacccaca catgccggaa tggcgctcgc gcaggcttcg cttggaaatc
     7621 acagacgagc gctcgaggac tatgaggctc ggctcgatct tcccacgatc tccgcggaag
     7681 aggagatcgg gctcctcgtc gagtacgcgc gcagcctgtt tcacgtgaac caacagccca
     7741 aggctatcga agcgctcagg ggcgcgcttg cgctgatgca tcaagacggg gaagttgccg
     7801 aggtcagtgg agcgactggg catccgacag aagggctcga gcgatacgag ccgctcatcc
     7861 tcgatcgcct ggcgctatac gagctcgacg cgcggcaaac cgagcactcg atggagactc
     7921 acgcgcagtt gcaaaggtct ttgcagtcgc accccgaagc ggattcggcg gtcaacgagg
     7981 tgaagacatg ggcccgtctc gcagcggccc atctcgccgg aggagatccc aagtctgcgc
     8041 tgggctttgc aacgaagggg caggagcgtt tgagcagtgc agacccgctg cgaccgaagg
     8101 atatcgatcg gaggattcga ccggtgacgc acgagttcgt ctatgacgag gaacaactgc
     8161 gcatcttact cgcagggttg gaggcgagcg ccgctcgaca gaccggagat tgggagcaag
     8221 cccgcgcgtc actcttgatc cggaaagaag ccctcgagga gcggtacgaa cgccaagagg
     8281 tcgacgaaga tgcgctcgag ctcgcgctca cctgcctaag acttgcggaa gttgctcaaa
     8341 aacgaagaga cctccagcaa accaagcgct atctcgaaga gggtcttgcc tacacggatc
     8401 gacatgcggg tagcactggc agcgacgtga gcgaagtcgg ctttcacctc ctacgcgcct
     8461 acgcggagct tcatttcagc ggaggcgtcg accttgcgtc ctaccaaagg gatctcgaac
     8521 aagacctact ccgctactat gttttcctgt cggaggtacg aaacccgcag tgggagaccg
     8581 agaggatccg tttcgaaatt tatctttccc ttctcagaat ggagaaggct cccgcgcctg
     8641 agccttgaag gatcgcagct aatccgcctt gaattcggcg acgatcgtcc cgtcttcgct
     8701 gtgaagccga agtacgtcgg cggagatctc atagcttctc gtttcttcga gcgcccttcc
     8761 gacctctcgc tctagatcca taccctcggg acaggctctc atggtcatgg cgattttcga
     8821 gaacgacagc ttgtctccgt cgagctggta gcctccgatc atttgattgc accccgcgaa
     8881 gcccgagatt cgttgctcgt cggccatgag cgtcaaagag ggcgccttgc ctcccgcccc
     8941 caaggttgcg ccctcagtcc ggagcttcac cagcatccaa cgcgtgtcgg tgagcgtggg
     9001 agcgttgggt ttgcgcgccg cagcaactcg gctcaccaga accttcaccg gatcgttggg
     9061 cgctccgtct tttgagccat ccacgccgaa cgccgggttc atctgcgtgc tggtgaacat
     9121 cagctgtcct tcgttttcga tgcgcgcccg tacgccataa cgtcctctag cgtcgagctt
     9181 ggacgggtcg tatgagagag tgacgcgata cggaggcgcg ccctggatcg ggaccgtcgc
     9241 ctcggcaatt ttttcggcgg caacgtccat cttcgcgctg tcttcaaagg tcactttgag
     9301 ctccgcagtc ggtggaagca gcatccgctc ccgatagaag acatccactt gcagctcgcc
     9361 cagagcgccg gcccccgggg ttggagtgat tggattgtcc ttgggcgtat cattcatatt
     9421 agcgctctgg caccccatga tcgcggccaa gaaaaccgct cctaccgtcc ttgcattctg
     9481 catggcccgc cctccgcgac tgtgtcatgt ggcatctgag cagaggcggc aagggatgcg
     9541 cacagttcgc tcgccctgct cgtccaggct cgcatgtctg cgcgggaaca tccacgcaat
     9601 cacgttgatt cgaaagacgc caagccatat tcttttctgc catgtccagc ccactccgcg
     9661 ctttgatcct tctcgcctcg ctgtctgcgc tttcgggcgc ggcgggctgc aataggagcc
     9721 caaaggctcc cgcatcccca agcgtcgatg cggccgatac catctatgtg ggcggcgacg
     9781 tcgtcaccat cgacgatgcg caaccaacgg ccgaagcgct cgctgtcaag gacggcagga
     9841 tcgttgccgt gggcgctcgt gctgacgtcg aaagagcgca caaaggcgcg accacacgga
     9901 tcgtggacct cggcgggaag accctcatgc cgagcttcat cgattcccac agccattatt
     9961 acaactccct gctggtggcg aaccaagcga agctctacgc gccgccatcg gggcctggca
    10021 aggatgtgcc gagcatcgtc gccgagctga agaagtttgc agcggaacgc aagatcccca
    10081 aaggcgagat gatcatgggt tacggctacg acgataccgt catgccagat ggccgattgc
    10141 tcaaccggga tgacctcgat gcagcgtttc cggacaaccc cgttcgtatc gatcacgtgt
    10201 cgatgcacgg ggccgtgatg aacagcctcg ccctcaagaa gtacgggttc agcgcgaaga
    10261 cgaaaacccc gccgggcggc gtcatcgtcc gcaagccagg cagcaaggag ccctacggcc
    10321 tgatcatgga aatggcgttc ctgcccgtga tcgagcgcca ggaaccgatg acgccggagc
    10381 aggagatcga gctcaccaag gccgggcaga tgctctacgc ggaggcgggc ctgaccaccg
    10441 ctcatgaagg tgcgactcac ctggcccagc tgcagacgat caagcgcgcc tcggaagcag
    10501 gcgccaacat catcgacgtc gtcgccttcc cgttcatcac ggacgtggac aagaccttga
    10561 aagagtttcc gctggagagc tggggcaagt acgaccagcg gctcaagatc ggtggcgtca
    10621 agatcagcat cgacggctcg ccacagggac ggaccgcgta cttcaccacg ccctacctca
    10681 caggagggcc cggcggcgag aagaactgga ccggcgacct tctggcctcc caggaagtga
    10741 tcaaccaggc tctcaagaag gtctacgacc tcgatgtgcc cgtgcttttc cacgccaatg
    10801 gcgacggcgc catcgattcg ctgatcacgg cgcacgagtt cgcggcggcc ggcgacccga
    10861 cccggtatcg gaatgtcact gcgatccacg cgcagttcac acgcaaagac cagattccaa
    10921 agttcgtgga gtataagata cggccgtcgt tctacaccct tcacacctac tattttgccg
    10981 aggcccacat tgccaaccgt ggtctgaagc aagcgatgta catcagtccg atgcacgacg
    11041 cgatcgacgc gggcctccac ccgaccaacc acaccgactt cgtggtcgcc ccgctggatc
    11101 aaatgatgat gctttggacg gccgtgaacc ggatttcccg agcgggtgcc gaaatcggtc
    11161 tcgaccaacg cgtcacgccc ctcgagggcc tgaaggcgat gacgatctgg gcggccgagc
    11221 aatacggcga agaaggctcc aagggctcgc tagcgccggg aaagctcgcc gacctcgtca
    11281 tcctcgacag gaaccctctc gaggtcgacc acatgacgat caaagacatc aaggtcgtcg
    11341 aaacaatcaa ggaaggcaag acgatctacg cgcgcagctc tgacgcggaa tgttgcgcgc
    11401 gttgagtggt gcatgcgctg aacgcgtcag tcgacgaccc gggcgaccaa gccggttcct
    11461 tccggcaatg tcacatcgcc acccgtgcga aggaggccga gcccgtacgt gaggacggcg
    11521 agagtaattg aacccttgat gcgactcttg ccctcaaagc gctgcggcgc atcgtcgagg
    11581 cgtacgactg ttccgttggc accggtcgtg ctcttgaacg acacctccat ccaacctggt
    11641 cgcccgatcc gcgggttctt tctgcggacg acactcgcca tcacccgcgc accctcgtga
    11701 acgagaactg tcccctcgct gttggtgatg tcctgcacga cggcgaagta gggttgctgc
    11761 cccgtcgtgc tcggctgctc ggaatccgca tttcgcgatt cgatttgaag aagcgcgacc
    11821 acgggaatgg tggttccggc aggaatgcct tcgtccgacg agctcgttgc caccgcctcc
    11881 gcttgagcgg tccgcacctg gcgcaccggt gagccgcagg ccgttaccga aatgagcgcg
    11941 aaggcggcgg cgatgaccgc gccggtccca aatcgtgccg tagaactctg cttttcaata
    12001 tgtttcatcg agtgctcctt tgttgcggcc cagtctgtgc catgcaaaac agcattgaaa
    12061 tgagttttta tgcacttggt cgttgcaatc atgcaaggat aagacgatgg agcgctggga
    12121 cgaccttcgc attttcctcg ctgcttttcg cgagggcagc tgcgcagcgg cgggcgctcg
    12181 cctaggcgtc aaccagagca caatcagccg acggatttca gctctggaga gagagctcgg
    12241 agttcgcctc ttcgaccgga ccccagaggg actggtgccc accgcggcgg cagaggagat
    12301 cgttcctcgt gccgagctct ttgaagtgac ggcagctgag ctgatcgggg ccatcgaagg
    12361 gctcgacacg cgcccaggcg gggtggtgcg gatcgcctcg ccagttttga tcgcgagcga
    12421 gctcctcgcc cctgccctcc ctgcctttct agacgaacag ccaggtctgc gcctcgagct
    12481 gattgtcggt gatgcaatcg tagacctcag tcgtcgagag gcggacctcg cgctgcgttt
    12541 cgttcgaccg gataggggcg acttgctcgt acggcgcgtc ggcaccctgc gcttcgcggt
    12601 cttcggatcg aagtcttatc tcgacatgca tcgcggccag cgggcagagg cgcttgcatg
    12661 gctcgattgg gacacgactc aggcacaact gcccgacgcg gcctggctgc gcgcggagtt
    12721 cccgggaatc gaaccggtgc tgcgcaccaa caacttgggt gtgagactac gcgctactcg
    12781 ggccgggctg ggtgtatctg tcttggctag atcgctcgcg gcgcgctact ccgagctcga
    12841 ggcactcgaa gatctgcccg tgattccaga catcccggtc tggctggttg ggcatcgtgc
    12901 attgcgcaac ctagctcgca tcaaggccgt ctgggcattc ctcgaagagc tcagcgccga
    12961 actgacatga gtccgacacg atcgccatac cgcgtgcatc agcgaggtcg gcaaggccgg
    13021 cagactcagt ggtggaaccc tgatgcttct tctttggtta gccgggattg caccgggtgc
    13081 cgggcaaagt agtcgagcgc ccgcttcatg tcgtcgagca ggagcgagcc cagatcgtgg
    13141 ctgaccccat gtcgcaccag aatgcgctgg atcgcgagat cctcacagtt cggagggaga
    13201 gtatacgccg ggacctgcca gccacggctt cgcaggcgat ccgcgaaatc gtagaggctg
    13261 aatccgggat cgactccgtc tttgatcttc cagcagagcg cgggaatgcc gctgcgcatg
    13321 tcgcctggat agatgatctc gaaggggccg agtttttcga tttcgccagc gatgtactga
    13381 gcggtgtcgt agcaagcctc gtggaccctg cgataatcct gctgcccaag gcgtaggaag
    13441 ttgtagtact ggcagacgac ttgcccaccg ggcctcgaaa aattcagccc gatatcgcgc
    13501 atgttgccgc cgaggtagtt cacccagaac accagctcgt cgggaaggtc atgctcatcg
    13561 cgccagatga tccatccaac gcccaacggg gacaatccga atttgtgtcc cgaggcattg
    13621 atcgatttga cgcgcgggag acgaaaatcc caaacgagat caggagcgca aaacggagcg
    13681 agaaagccgc cactggcgcc gtccacgtgc agcgggatgt cgagcccggt cttcttttcg
    13741 tactcgtcca aggccttggc cacggcttgc accggctcgt actgccccgt gaaggtgacg
    13801 ccgagcgtcg gaacgacacc gatggtgttc tcgtcacatc gcttgatgac ctcttccggc
    13861 gtcatcagga gtcgaccatg ctccatggga atctctcgat gctcgatatc ccagtagcgc
    13921 gtgaacttgt gccagcagac ctgcactggg ccggtaacca gatttggctt ctcgatggac
    13981 ttgcccgccg ccttccgagt gttctcccag cggcgtttca tcgccatccc acccagcatc
    14041 gccgcttcgc tagagcccgt cgtcgagcat ccaatcgtgc ttgctgcctc cggtgaattc
    14101 caaagatcgg ctagcatgtg gacgcagcgc gcctcgatct cggcggtctg tgggtattcg
    14161 tccttgtcga cgatgttctt gtccatgcac tcgtccaaga gcttgtgcat ttcgggctcc
    14221 gcccaggttt gacaaaacgt ggccaggttc tgacgcgagt tgccatcgag catgagctcg
    14281 tcgtgcaccg ctgcgtagac ggccttggca agcgactcgg attttggaaa gcgatacttg
    14341 ggcatgccca ccagtagctc cgaggatgcg tatatctcat catcgagctg ctctcggatc
    14401 gattccttgt cgtgcagagt catctcattc tcctggtgct ggtgtttggg tccgtctcga
    14461 atgcgatcgt tggggacggt ttggctcatg ttctcacaac gagcaatgag ctcgaggcca
    14521 cgagaatctt cgctttgcag ccggctcacc tattcgggga gctcaatgcc gctcgagggc
    14581 caggtcgtgc gcctcgtcgt agtggcggcc gaggttgtgc cagtcgaaat gctcgctgaa
    14641 agcttcgacc gagttgcgga gaatcacgcg ctcccgtcgg ctgagcgatg cgaacctgaa
    14701 gagccgatcg gtcagctcgt cggccgcgcg gtggaagtcg ttgtgccggc ggggcacgac
    14761 gtagatgccc ttggacgcgt ggtccggcaa ttgttgcttg aggtacgagc cgaatcccga
    14821 caggtcgctg gtcaccgcgg gcacgccaag cgctattgct tcgagcggcg tgtagcccca
    14881 gggctcgtag aagctcggga acacgcccag atggcagccg cggacgaact gctcatattc
    14941 cattccgaat agagggctgg tggcctgcac gaaatcggga tggaagacga ctttgaccgg
    15001 gtcgtcctgc tggttgaaca gtcgacaggt ccgaagctgg ttcagcacgg ggtcgtgggc
    15061 gtcgtcgatg aggtcgtggg tcacgatgcc cgggagggcc tcgctcttcc aagcatggat
    15121 cgcgcgacgg agacggagcc gccagtagtc atccatcagg atgttgagat cggggatctc
    15181 gccggtcgag gcggcgcgga aaagcccgtc gccgacggac tccttcaccg cgtcggaagc
    15241 ggtgcggaat tcgcgaagca ttgcgcgact ctgcaacgcg cggacgttga tcgaatggaa
    15301 cggagccttg gtgatgatga agaacacgac cgtcacgggg ctccccgtct cccgaagccg
    15361 gtggttgagc cgggccaacg cctcgatggt caggtccatg cccttgttga ggtactcgta
    15421 ccgacccgag gtgaacatgt agagcgtgtt gtcgaggtcg aagttgtaac taggaaagaa
    15481 gtgccccatc gagaacctgt gtagccgttc cttgtgttgc tggtggagcg tctggaactc
    15541 gtgtgtcgcg gtgaaccggc ggatgttcag cccgttgggc agcagcatgt cgggcgtccg
    15601 cccgagcaga tgggtgcact cctcggcggt gaggtccgac accgtcgtga agacgtgtga
    15661 cgcgtgcgcg gcggctttct cgatctggtg ctgagcgtcg atgttgaagt tcttggcttc
    15721 ctgatctccg tcgaacgagg gcagctggtc gtagaacgag gtattgcctg tcgctaaata
    15781 gcggccgacc agagttgcgt gcgtggtaaa tacgagcgag cctggccagc gctcctttcg
    15841 cagcatcgga atggccagac cggcctgcca ctcgtggaag tgagccacga ctcggcggcg
    15901 ctgcgaatcc tcctgcgcga gcaccgacag cagagcccgg cacatctcac caaatgccac
    15961 ggcgtcgctc acgcccggct cgtcgctcgc ggggatcccg tggtcggccc agacccggta
    16021 cttgatctcg ttgaccatcg cgccgaccga accgacgtcc agcagaacca cgtgcggacg
    16081 gccgctaacc agccaacgtc cgtagtgtgc acgatagccc aactctcgca gttgctcgac
    16141 cgcttggccg acctggccaa acagcggcgc gggctcgaac tcgacctccg cggtctgcgg
    16201 gttgtacgg
//
LOCUS       DASRZF010000443         3209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_341030, whole genome shotgun sequence.
ACCESSION   DASRZF010000443 DASRZF010000000
VERSION     DASRZF010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341030"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="VFG22_19455"
     CDS             complement(<1..598)
                     /locus_tag="VFG22_19455"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6418483.1"
                     /translation="MIPDSYFDSFHTAKYRSENPVQRFLIRRFIEKLAGLFDLTQPSG
                     SVLEIGCGEGFVAGQLSARHSGESYVGVDLDAKDIENLRAKFPGVEAHQGSIYDLSFL
                     GREFDVVVCAEVMEHLEEPRAALEQIIERKPRWVILSVPHEPWFCLSNFARGKNITRL
                     GNDPEHVNLWGRRGFRKVIESHLVVERHETSYPWQLVLA"
     gene            complement(585..1583)
                     /locus_tag="VFG22_19460"
     CDS             complement(585..1583)
                     /locus_tag="VFG22_19460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET6418484.1"
                     /translation="MGLGEQIKGAVDAVVPKSRVPDVTLVVPGLNEAESLPLLAAQVK
                     EALGTEISYELIFIDDGSTDDSWRVIQALNQSDPAVRGIRLRKNFGKAMALAAGFERA
                     RAEIVVMMDADLQDDPADLPQMIEKIRGGLDVVVGWKVERHDPLNRRIFSRIFNGTVG
                     YVIGVHLHDMNCGFKAYRQEVLQSIPIYGDLFRFIPVLASWQGFSVGEMPVHHRARQF
                     GSSRYGLERILRGFFDLLSVSFLTKYSRKPMHLFGLLGLLFTTLGLLICGYLSVVWLQ
                     GGAIGSRPLLLLGVLLILVGVQSFSIGLLGEFMTFQYHRKHEREHLPVREVIGDPR"
     gene            complement(1650..2867)
                     /locus_tag="VFG22_19465"
     CDS             complement(1650..2867)
                     /locus_tag="VFG22_19465"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET6418485.1"
                     /translation="MSETPPDLKPGLPFSALNKLDRLSEKMIPIDSERVMKMAQRTTG
                     LDDWGNGGFRARLDAAVDGLNEAELNTTGLFGARYVLNWHLGNRLRVVDFAKRHPELD
                     DVDIERPVVITGFFRTGTTFLHNVLAADPNNRVAQAWELAYPVGRLGDPLGDVTWRRA
                     QAKFTFGFNQAAIPDQGVAHNVTADSYEEDFFLLENDMAVLTFWVGFAAYGYAREMLN
                     WDMMEPYRFHKLQLKILNAQQSAKRWVLKCPWHIWNMDALMAVYPDAQVIFTHRDISK
                     ALASHCSLSARMASKLKRSLDVKELGEFWLDYTRIGLERAMESRKNIPESQLYDVRLR
                     DLMANPMTVLQDIYAHFDLEFNEEIAGLLEARIGEKPTSQEGEHEYAIEDFGLTDERV
                     RETLKTYNERFGV"
     gene            complement(3015..>3209)
                     /locus_tag="VFG22_19470"
     CDS             complement(3015..>3209)
                     /locus_tag="VFG22_19470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418486.1"
                     /translation="DHDQPEDKEGVGISFALGLRYSFGKGDAAGLLIPPNYDPSAISV
                     IAVPTKINELAVNIAVNVAF"
BASE COUNT          620 a          996 c          975 g          618 t
ORIGIN      
        1 tcgcgaggac gagctgccac ggataggagg tctcgtgcct ctccaccacg agatgcgact
       61 cgatcacctt ccgaaagccg cgacgccccc agaggttcac atgctcgggg tcattgccga
      121 ggcgcgtgat gttctttccc cgtgcgaaat tgctcagaca aaaccagggc tcgtgtggca
      181 cggacagaat cacccagcgg ggctttcgct cgatgatctg ttcgagcgcg gctctgggct
      241 cttccaggtg ctccatcacc tccgcgcaga cgactacgtc gaattcccga cccagaaacg
      301 acaggtcgta gatgctccct tggtgggctt cgacgccagg gaacttcgct ctcaggttct
      361 cgatgtcttt cgcgtcgaga tccacgccga cgtacgactc accggaatgc cgggcagaaa
      421 gctgaccggc cacgaatccc tcgccgcatc cgatttccag tacggatccc gatggctgtg
      481 tgaggtcgaa gagcccagcg agcttctcga tgaaacgccg aatgagaaac cgctgcacgg
      541 ggttctcgct ccggtacttg gcggtatgaa agctatcgaa gtagctatcg gggatcaccg
      601 atgacctcgc gtactggcag gtgctcacgc tcatgcttgc gatggtactg gaaggtcatg
      661 aactccccca gtagcccaat ggaaaacgac tgcacaccga ccaggatcaa gagcacgccg
      721 agcagcagta acggccgcga gccgatcgct ccgccctgaa gccaaaccac ggagaggtat
      781 ccgcaaatca acaatccgag cgtcgtgaag agcaagccga gcaagccgaa gaggtgcatc
      841 ggcttgcggg agtacttggt gagaaacgag acgctcaaga ggtcgaagaa gccccgaaga
      901 attcgctcga ggccgtagcg cgagcttccg aactgtcgcg cacgatggtg cacgggcatc
      961 tcgccgacac tgaagccttg ccaggatgcc agcaccggga tgaagcgaaa caagtcacca
     1021 tagatcggga tcgactggag cacttcctgc cggtacgcct tgaaaccgca gttcatgtcg
     1081 tgcaggtgca cgccgatgac gtagccgacg gtcccgttga atatgcgcga gaagatccgg
     1141 cgattgagtg gatcgtgccg ttcgactttc cagccgacca cgacgtccaa gccccctctg
     1201 atcttctcga tcatctgcgg taggtccgca gggtcgtcct gaaggtcggc gtccatcatg
     1261 accacgatct cggcccgggc gcgctcgaag cctgcagcca gcgccatcgc cttgccgaag
     1321 ttctttcgga ggcggatccc ccgcacggcc ggatcgcttt ggttcagagc ttggatgacc
     1381 cgccagctgt cgtccgtgct cccatcgtcg atgaagatca gctcgtacga aatctccgtc
     1441 ccgagcgcct ccttcacctg cgccgcgagc agcggcaggc tctccgcctc gttgaggcct
     1501 ggaacgacta aggtcacgtc cggcacccgc gactttggca ccacggcgtc gaccgcaccc
     1561 ttgatctgct ctcccaatcc catcacggag ggctttttag gctccctgcc gtccgcgcac
     1621 aagccgcagc tccaaacgag cgcgaacccc tagaccccga agcgctcgtt gtaggtcttc
     1681 agggtctccc ggacgcgctc gtcggtgagg ccgaagtcct cgatcgcgta ctcgtgctcg
     1741 ccctcttgcg aggtcggttt ctccccgatt ctcgcctcga gaaggccggc gatctcctcg
     1801 ttgaactcca ggtcgaagtg cgcatagatg tcctgcagca cggtcatcgg gttggccatg
     1861 aggtcgcgaa gccggacgtc gtagagctgc gactcgggga tgttcttccg ggactccatc
     1921 gctctctcta gaccgattct cgtatagtcg agccagaact cgccgagctc cttcacgtcg
     1981 aggctgcgct tgagcttcga ggccatcctg gcggacaagc tgcagtggga cgcgagggcc
     2041 ttgctgatgt ctcgatgggt gaagatgact tgggcgtccg gatagaccgc catcagcgca
     2101 tccatgttcc aaatgtgcca ggggcacttc aagacccaac gcttggcgga ttgctgcgcg
     2161 ttcaagatct tcagctggag cttgtgaaac cgatacggct ccatcatgtc ccagttcaac
     2221 atctcccgtg cgtagccata ggccgcgaac ccgacccaaa acgtgagcac ggccatgtcg
     2281 ttctcgagca agaaaaagtc ttcctcgtaa gagtcggcgg tgacgttgtg ggcgacgcct
     2341 tgatcgggga tcgcggcttg attgaagccg aacgtgaact tggcctgagc gcgtctccag
     2401 gtgacgtctc cgagagggtc gccgagtcgc ccaacgggat acgcaagctc ccaagcctgg
     2461 gctacccggt tgttcgggtc tgcagccagc acgttgtgaa gaaacgtcgt tcctgtcctg
     2521 aagaagccgg tgatgacgac gggtcgctcg atgtcgacgt cgtcgagctc cgggtggcgc
     2581 ttcgcgaaat cgacgacgcg aagcctattg ccgaggtgcc agttgaggac gtagcgtgcg
     2641 ccgaagaggc ccgtggtgtt gagctccgcc tcgttcaggc cgtcgaccgc cgcatcgagt
     2701 cgcgccctga acccgccgtt cccccaatcg tctaggccgg tcgtgcgctg cgccatcttc
     2761 atgacgcgct ccgagtcgat cgggatcatc ttctcgctga ggcgatccag cttgttcagg
     2821 gccgagaaag ggagcccggg cttcagatcg gggggggttt cgctcatcgc cgctctagta
     2881 gaacgcggaa ccctggggtg tcaaagcaag ggtttcgccc tgagccgagc ggtcgcacag
     2941 gggtcgccaa cggtcttttg caggcgctca gcggtcgccg gctcgccggc gcgcgttcag
     3001 acaggcgctg cgattcagaa cgcgacattg accgcgatgt tgactgcgag ctcgttgatc
     3061 ttggtgggga ccgcgatgac cgagatggcg ctagggtcgt agtttggggg aatgagcagg
     3121 cccgccgcgt cgcctttgcc aaacgaatat cgaagaccaa gagcaaagga gatgccgact
     3181 ccctctttgt cctcgggctg atcgtgatc
//
LOCUS       DASRZF010000444         7558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_4811916, whole genome shotgun sequence.
ACCESSION   DASRZF010000444 DASRZF010000000
VERSION     DASRZF010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 7558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7558
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4811916"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VFG22_19475"
     CDS             complement(<1..208)
                     /locus_tag="VFG22_19475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418487.1"
                     /translation="MTRSNPSLRYRLLRGWALQSRKVQYFTRRIEDAVTVWTGGILDG
                     LLRDAEKEALSIDLYDASFRPDNDH"
     gene            complement(205..1026)
                     /locus_tag="VFG22_19480"
     CDS             complement(205..1026)
                     /locus_tag="VFG22_19480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418488.1"
                     /translation="MARGNLEQHEIFLSDLRVRVQSSCRRTNRYLRRHWSAAKLPMLP
                     TEVSCDIEVIADASPRIIVDGELVWADGVAEDLVAGFELWLYRAALAKHRGRFAVFHA
                     AALVSDGATVVFSGPSGAGKSSLALAAVRRGWKYFSDEFVVTDGQSVCGWPRAIRFDP
                     TEPGAPCPDYLVDLDTDQDAVESPQDVAPPYYAVRGNALQRVACSAREVRFVSIERGT
                     DTTLSRVSASVGLKYWTEAAFIEPTMSLGALVGEGRTWRASWRHPEELIDLIETT"
     gene            complement(1008..1301)
                     /locus_tag="VFG22_19485"
     CDS             complement(1008..1301)
                     /locus_tag="VFG22_19485"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HET6418489.1"
                     /translation="MPDASDRRVPKRKDTLTHEVTDTDEVVIYDGSGPQLLVLNDIAA
                     GVWLMIDGARTVDDITAEILNHLEAEPATVTRDVLAFLGQLEQRKLIAWQEAT"
     gene            complement(1294..2457)
                     /locus_tag="VFG22_19490"
     CDS             complement(1294..2457)
                     /locus_tag="VFG22_19490"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET6418490.1"
                     /translation="MDEAGGTVGEATMNATGYFLPDDWILWHSEGSLEATKLSSGDQI
                     RIEGTPEHDVLNGELPASIWDSLARFEPLLGQLRERLQERALQPLTRETVLRGSGWKQ
                     LFVELTAKCNEQCVHCYAESSPARTEALAWPEISKVLEDAKALDFGLVQFTGGDPLIS
                     PHCVQAVEFARDLGIPRVEIYTNGLALRGRIYERLRELAPSFAFSFYSHNPDTHDAIT
                     QTPGSHARTARAIRRAVDDGLNVRVGVISMERNQHDASKTYEYLLGLGVAGNSIGFDQ
                     VRDVGRGDAAPIETDTRPVGVRSGGVESARPRNFGGSAAVGYDGKVYPCIFSRHLPLG
                     SIHDTSLEAILRSAEPIASKKRSLLTARERWGEKLSCWECQARSVLLDGSCNA"
     gene            complement(2450..2656)
                     /locus_tag="VFG22_19495"
     CDS             complement(2450..2656)
                     /locus_tag="VFG22_19495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418491.1"
                     /translation="MTDEGETSELDRARRHFLRATAYMAPAILATITLNKAHAQASCM
                     PSTCNPGTCNPNGCNPLNCMPAGG"
     gene            3384..3575
                     /locus_tag="VFG22_19500"
     CDS             3384..3575
                     /locus_tag="VFG22_19500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418492.1"
                     /translation="MELLDRLGGMLFSSKADECKTPRAAGFAVLWNVNVNYLADLSKE
                     ITQLLVGRGKVEVPYEYLA"
     gene            complement(3681..4577)
                     /locus_tag="VFG22_19505"
     CDS             complement(3681..4577)
                     /locus_tag="VFG22_19505"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002627103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HET6418493.1"
                     /translation="MVSDTFDASKWIEVPGFDLQDITYHRAKEHGTVRIAFDRPEVRN
                     AFRPGTVDELYRALDHARQTSDVGCVLLTGNGPSPKDGGWAFCSGGDQRIRGKDGYKY
                     ETDSAGEIDPARLGRLHILEVQRLIRFMPKVVIAVVPGWAVGGGHSLHVVCDMTIASE
                     EHALFKQTDPDVASFDSGYGSALLARQVGQKKAREVFFVGLNYSAREAAAMGMVNAVV
                     PHAELENFALSWAREINSKSPTAMRMLKFGFNMIDDGLVGQQLFAGEATRLAYGTEEA
                     QEGRDAFLEKRPQDYKKFPWHY"
     gene            complement(4585..5199)
                     /locus_tag="VFG22_19510"
     CDS             complement(4585..5199)
                     /locus_tag="VFG22_19510"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6418494.1"
                     /translation="MSPSNRPSDPSRTRLSPEDRQQQLLRVTSDIIAKDGVDSVRIPH
                     VAAAAGITRPVVYKFFPNRHELIKGVLEDFREEFANRAPNVFDADDDADVASIARAFI
                     DAACDTIEEKGSGGWQLVRSVGPDREISAISKGFRRQLVQPWLIPLRELTGVDDAEAQ
                     ALADMMLTGAGEILQRWIEGEFSRQQVATLLGRIILAVLNEFTE"
     gene            complement(5264..5605)
                     /locus_tag="VFG22_19515"
     CDS             complement(5264..5605)
                     /locus_tag="VFG22_19515"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HET6418495.1"
                     /translation="MESAAEFFEKFEQRAAGNTDAATAPNAVYQFKITGDGGGEWNLD
                     LTKGKTGDFVSKGTHASPGATITVSAEDWLGMLNGSLNPMQAFMSGKIKIDGDMTLAM
                     SLQQVMNLAQD"
     gene            5894..7126
                     /locus_tag="VFG22_19520"
     CDS             5894..7126
                     /locus_tag="VFG22_19520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HET6418496.1"
                     /translation="MDALNQADTGWLMVSCALVMLMTPGLALFYGGMVNSRNVLSSFM
                     HSFIALGIITLQWVVIGYSLSFGSDAGGGFIGGFQYAFLSGVGLEPQEGSTIPHILFM
                     AFQMMFAIITPALISGAIAERMKFSAYFLFIILWATFVYDPICHWVWGPDGWLGARGA
                     LDFAGGTVVHLSSGAAALVCALVIGRRSRPGPPHNLTLTLLGAGMLWFGWFGFNAGSA
                     LAANANAALALVNTHLAAGAAAFAWAIVEWIKQGKPTALGVASGLVAGLVCITPAAGF
                     VGPTSAVLMGLIAGPVCYGAVMLKGTLGYDDALDAFGIHGVGGALGAVLTGVFASAVW
                     GGTDGLIGGNSQLFIENIIGVLAAAAYSVVVTLVILKVLDATVGLRVSKDQETEGLDT
                     NLHGEEGYHMGGAGALTE"
     gene            7261..>7558
                     /locus_tag="VFG22_19525"
     CDS             7261..>7558
                     /locus_tag="VFG22_19525"
                     /inference="COORDINATES: protein motif:HMM:NF012400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097159 - organic cyclic compound binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5-like heme/steroid binding
                     domain-containing protein"
                     /protein_id="HET6418497.1"
                     /translation="MRDYSLDELPEYDGSDPSKPLLVGVRGNVYDVTRGRDFYGPGGP
                     YAMFAGKDCTRALAKVSFDEELFTGDIRDLEAEELEKLDEWIEMFEGKYRRVGRL"
BASE COUNT         1352 a         2323 c         2297 g         1586 t
ORIGIN      
        1 gatgatcgtt gtcgggccga aacgaggcat cgtagaggtc gatggacagg gcttcctttt
       61 ccgcgtcacg aagcagtccg tccaagatgc ctccggtcca aacggtgacc gcgtcctcga
      121 ttcgacgcgt gaagtactga actttgcgac tctgcagcgc ccagcctcga agcaatcgat
      181 atcgtaggct tgggttgctt cgtgtcatgt cgtttcgatc agatcgatga gctcttctgg
      241 gtggcgccac gacgcgcgcc aagtccgtcc ttcgccgacc agcgcgccca aggacatcgt
      301 tggctcgata aacgcggcct ccgtccaata cttcaggccg acactcgctg agacgcgcga
      361 tagcgtcgta tctgtaccgc gctctatgct aacgaaacgc acctcgcgcg cagaacaggc
      421 gacgcgttgc aaggcgttgc cccggacggc gtagtagggt ggtgcaacgt cttgcggaga
      481 ctccacggcg tcttgatcgg tatcgaggtc gacgaggtag tcaggacaag gcgcgcccgg
      541 ctccgtagga tcaaagcgaa tcgcgcgcgg ccacccgcac acgctctggc cgtcggtgac
      601 cacgaactca tcggaaaaat acttccaacc gcgccgaacc gccgcgagtg caagcgagct
      661 cttcccggcg cctgagggtc cggagaaaac gacagtcgct ccatccgaca ccagcgctgc
      721 tgcgtgaaat acagcaaatc ggcctcggtg cttcgcgagc gccgcgcggt agagccagag
      781 ctcgaaacca gcaaccagat cttcggcgac accgtcggcc cacaccagtt cgccatccac
      841 gatgattcga ggtgatgcgt cggcgatcac ttcaatgtcg cagctgacct cggttggcag
      901 catcggcagc ttcgcggcgg accagtgacg tcgcaggtaa cgattcgttc tcctgcagct
      961 gctttgtacg cgaacacgaa ggtcggaaag aaagatctcg tgttgctcta ggttgcctct
     1021 tgccatgcga tgagctttcg ttgctcgagc tggcccaaga acgcgagcac gtcacgggtc
     1081 accgtcgcag gctccgcctc gagatggttc aggatttcgg cggtaatgtc gtcgactgtt
     1141 cgtgcgccgt cgatcatcag ccaaacgccg gcggcaatat cgttcaagac gagcaattgc
     1201 ggaccgctgc cgtcgtagat cacgacttcg tcggtatcgg tgacctcatg cgtgagtgtg
     1261 tccttgcgct tgggcacgcg tcgatcggaa gcatcaggca ttgcagctcc catcgagcag
     1321 tacggatcgg gcctggcact cccaacagga gagcttttca ccccatcgtt cgcgggccgt
     1381 cagcaggctt ctctttttcg aggcgatcgg ctctgcagac ctgagaatcg cctcgaggct
     1441 ggtgtcatga atcgatccga gtggcaggtg ccgactgaag atgcacggat acaccttacc
     1501 gtcgtagccc accgcggccg accccccgaa gtttcgcggt cgcgcgctct cgacaccacc
     1561 gctccggacg cccaccggtc gtgtatcggt ttctatgggt gcggcgtccc ctcgaccgac
     1621 gtcgcgcact tggtcgaaac cgattgagtt gccagccacg ccgagcccca gcaagtattc
     1681 gtacgtcttc gatgcatcgt gttggttccg ctccatcgag atgacaccga cgcgtacgtt
     1741 caatccatcg tcgaccgcgc gtcgaatcgc tcgcgcggtg cgtgcatgac tacctggcgt
     1801 ctgggtaatg gcgtcatggg tgtcgggatt gtgcgagtag aacgaaaacg caaacgaggg
     1861 cgcgagctcg cgcaagcgct cgtagatcct tccgcgaagc gcgaggccat tcgtgtagat
     1921 ctcgacgcgc ggaattccga gatctcttgc aaactctacg gcttggacgc aatgggggct
     1981 gatcagagga tctccacctg taaattggac caggccaaag tccagcgcct tcgcgtcttc
     2041 gagtaccttg ctgatctccg gccaggcaag tgcctccgtt cgggcaggcg acgattcagc
     2101 atagcaatgg acacattgct cgttgcactt cgcggtgagc tctacgaaaa gctgtttcca
     2161 tccggaaccg cgcaagacgg tttctcgcgt gagtggttgt agcgcccgct cttggagtcg
     2221 ttctcggagt tgccccagga gaggttcaaa ccgcgccagc gaatcccaaa tggatgcggg
     2281 cagctcgcca ttcaagacat cgtgctcggg cgtgccttcg atccggatct gatctccgct
     2341 gctcagcttt gtggcttcga gcgatccctc gctgtgccag aggatccagt cgtcgggcaa
     2401 aaagtagccg gtcgcgttca tcgttgcttc gccaaccgtt ccacccgctt catccaccgg
     2461 cgggcatgca gttcagtggg ttacacccgt ttgggttgca ggtccctggg ttacaggtcg
     2521 agggcatgca cgacgcctgc gcgtgtgcct tgttcagcgt gatggtcgcg agtatcgccg
     2581 gtgccatgta cgccgtggcc ctcaggaagt ggcgccgagc gcgatcgagc tcgctggttt
     2641 ccccctcgtc ggtcatcgtt gctagataca gcaagtccga ctcacacgcg acccgaagcg
     2701 agctttcggc acgccctaca acgacataga gggactcccg aaattcacgc cttcgcgcgc
     2761 gagcgaacgg gttcgctttt catcagggca gggcatccgc cgcgaatcag agagtccgga
     2821 acgtcccttt gctccggtcc agagcgccta gatccaagtt aactacctgt aacatttgaa
     2881 taattgtgac cgaaaatacc tgttgacaac cgtgtttgcg aacctttacc gtaggactgt
     2941 cggcggctga tttgttaccc gccggcgggc cagcagcgat tcacactcct tggggtacaa
     3001 caaaacgggc actcgggcgg ggttcacacc tcgcccgaga actttaaggt cttcgtcttg
     3061 gtcgccgacg cacccgagcg gttcggcacg ctacgggcga aacgccgtta gaggcttcgc
     3121 ttctttccgc gaagcccgcg gcgactgccc ttgggctttt tcggtcggtc gaacgaacga
     3181 gcgtcgccac cgccgccgcc gccgcgatcg taaccaccac cacggtcgta gctgccacca
     3241 cggtcgaagt caccaccgcc gggttcacca ccgcgattga aacgatccgg tggggggccg
     3301 ccgaaatcac gcctctgccg cggcgggcgg ggccgatcct ccgctatatc caggcggatc
     3361 tcgcgtccac cgacttcgcg tccgtggagc ttctcgaccg cctgggcggc atgctcttca
     3421 gcagcaaagc ggacgaatgc aaaaccccga gggcggccgg attcgcggtc ctttggaatg
     3481 tgaacgtcaa ctacctcgcc gatctctcca aagagatcac gcagctcttg gtcggtcgtg
     3541 gcaaagttga ggttccctac gaatatcttg cttgagatga cttactccgt tctccgatgt
     3601 gctcaattgg cacgttttag gactgtgtcc aagtgagcta gtgtaaccca gaactggttt
     3661 tttttccctc agactcgcct tcagtagtgc caggggaact ttttatagtc ctgagggcgc
     3721 ttttcgagga aggcgtcgcg cccctcctgg gcttcttcgg tgccataggc gagccgggtc
     3781 gcctctcccg cgaagagttg ctgacccact agaccgtcgt cgatcatgtt gaagccgaac
     3841 ttcagcatcc gcatcgcggt ggggctcttg gagttgatct ccctggccca ggagagggcg
     3901 aaattctcga gctcggcatg cggcaccacc gcgttcacca tccccatcgc cgcggcttcc
     3961 ctggcgctgt agttgagacc cacgaagaag acctcgcgcg ccttcttctg accgacctgc
     4021 ctcgcgagga gggcagagcc atacccgctg tcgaagctgg ccacgtcggg atcggtttgc
     4081 ttgaagagtg cgtgctcttc gctggcgatg gtcatgtcac aaaccacgtg gaggctatgc
     4141 cccccgccaa cggcccaacc tggcaccact gcgatcacga ccttgggcat gaatcggatg
     4201 agacgctgaa cctcgaggat gtgcaatcgg ccgagcctcg ccgggtcgat ctctcccgcc
     4261 gagtcggttt cgtacttgta gccgtccttg ccgcgaatgc gttgatcgcc gccggagcaa
     4321 aacgcccagc ccccgtcctt gggtgaaggg ccgttgcccg tcaggaggac gcagccgaca
     4381 tcggaggtct gccgtgcgtg gtcgagcgcg cgatagagct catcgacggt tcccggacga
     4441 aacgcattgc gcacctccgg acggtcaaaa gcgatgcgaa ccgtcccgtg ctccttggcg
     4501 cgatggtagg tgatgtcttg cagatcgaag cccgggactt cgatccactt cgacgcatcg
     4561 aatgtgtcgg aaaccatgct tctcttactc cgtgaactcg ttcaggacgg cgaggatgat
     4621 tcgtccgagc aacgtggcca cctgttgacg ggagaattct ccttcgatcc agcgctgaag
     4681 aatctcgccc gcgcccgtga gcatcatgtc ggcgagcgct tgcgcctccg cgtcgtctac
     4741 acccgtgagc tcacgcagtg gaatcagcca tggctgaacc agttggcgtc gaaaaccctt
     4801 gctgattgcg gagatttcac gatcagggcc aaccgatcgg accaactgcc aaccccccga
     4861 tcccttttct tcgatcgtgt cgcaggcggc atcgatgaat gctcgcgcga ttgaagcgac
     4921 atctgcgtcg tcgtcggcat cgaagacgtt cggcgcccgg ttcgcgaact cttctcggaa
     4981 gtcttcgagc actcccttga tgagctcgtg tcggttcggg aagaacttgt acacgaccgg
     5041 gcgcgtgatg cccgctgcgg ctgccacgtg cggaatgcgc acgctgtcca caccatcctt
     5101 ggcaatgatg tccgaggtga ccctcaacaa ttgctgttgc cggtcttcgg ggctcagcct
     5161 ggtgcgcgag gggtcgcttg gtcgattgga tggagacatt ggcttacgaa aaagggtggc
     5221 ccccgataga agccaccccg atcgaatcag acttccgttc ggctcaatct tgagccaggt
     5281 tcatcacctg ctgaaggctc atcgcgaggg tcatgtcgcc gtcgatcttg atcttgcccg
     5341 acatgaatgc ctgcatcggg ttgagcgaac cgttgagcat tccgagccag tcttctgcgg
     5401 acacggtgat cgtcgcgccc gggctggcgt gcgttccctt gcttacgaag tcgcccgtct
     5461 tgcccttcgt gaggtccagg ttccattcgc cgccaccgtc gccggtgatc ttgaactggt
     5521 agactgcatt gggagcggtg gccgcgtcgg tgttgcctgc ggctctctgc tcgaactttt
     5581 cgaagaactc agctgcggat tccattgata atgcctctct tgttatgtcg gccgccgggt
     5641 tcacgtgcga atcggtgtgt cgggccggac gggagttgta accgaaatca cggcggcatg
     5701 cacccctgta aactgacgaa aagcagtgtg atttcagggc cggtcacgga gggggtcggg
     5761 gcggctccgg gccgggcccg gtctttgggg ctgaggcgct cccccttgct ttgaaacaag
     5821 ccggaaacgt ccgttacatc cggcggaaac agctcggatc tagtctctgc gcacttcgaa
     5881 ggaggaaccg gggatggatg ctctcaatca ggcggacact ggatggctca tggtgtcgtg
     5941 cgctctcgtc atgctgatga caccgggttt ggcgctcttc tacggcggca tggtgaactc
     6001 gagaaatgtg ctctcgtcgt tcatgcacag cttcatcgcg ctgggaatca tcacccttca
     6061 atgggtcgtg atcggctact cgctctcgtt cggctcggat gcaggcggtg gcttcatcgg
     6121 tggcttccaa tatgcctttc tgagtggtgt cgggctcgag ccgcaggagg gctccacgat
     6181 cccgcacatc ctattcatgg ccttccagat gatgtttgcg atcatcacgc ccgccctgat
     6241 ttcgggtgca atcgccgagc ggatgaagtt ctcggcctac ttcctgttca tcattctttg
     6301 ggcgacgttc gtttatgatc cgatctgcca ctgggtctgg ggaccggatg gctggctcgg
     6361 cgcgcgcgga gcgctcgact ttgcaggcgg caccgtggtt cacctgtctt ccggcgccgc
     6421 cgcgctggtc tgtgccctgg tcatcggtcg aaggagccgc cccgggccgc ctcacaacct
     6481 cacactgacg ctgctcggag cgggcatgct ctggttcggc tggttcggat tcaatgccgg
     6541 cagcgcgctt gctgcgaacg ccaacgcagc gctcgccctc gtcaacacgc acctcgcagc
     6601 cggtgcagct gccttcgcct gggcgatcgt cgagtggatc aaacaaggga agccgacggc
     6661 gctcggcgtc gcctcgggcc tggtggccgg cttggtctgc atcactccgg cggctggttt
     6721 cgtgggcccg acctcagcag tgctgatggg tctcatcgca ggcccggttt gctacggggc
     6781 cgtcatgttg aagggcacgc ttggctacga cgacgcgctc gacgcattcg gcattcatgg
     6841 cgtaggcggc gcgctcggcg ccgtgctcac cggggtcttt gcgagcgccg tctggggcgg
     6901 caccgacggg ctgatcggcg gcaacagtca gctcttcatc gagaacatca tcggtgtgct
     6961 tgcagcagcg gcatactcgg tcgtggttac cttggtgatc ctgaaagtcc tagacgccac
     7021 cgtcggcctg cgcgtcagca aggaccagga aaccgaaggt ctcgacacca acctgcacgg
     7081 tgaggagggc taccacatgg gtggcgctgg agcgctcacc gagtagcctg gcgcagcgac
     7141 cgaaggccca ttgtctacgg cgcaacttcg tcgtaggcct caggcgtggg tctttctcgc
     7201 ttgatggcac gtctgtttcg aagggagccg ccgcggcgcg cgcctttgcc tgaggccgag
     7261 gtgcgtgatt actccctcga cgagctgccc gaatacgacg ggtctgatcc gagcaagccg
     7321 ctactcgtcg gcgttcgcgg caacgtttac gacgtgactc gcggtcggga cttctacggt
     7381 cctggcggtc catacgccat gtttgccggc aaggattgca cgcgcgcgct tgctaaggtg
     7441 tcctttgatg aagagctgtt cactggcgac attcgagatc tcgaagccga ggagctggag
     7501 aagctcgatg agtggatcga gatgttcgaa ggaaagtatc ggcgggtagg gcgtctcc
//
LOCUS       DASRZF010000445         2540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiales bacterium isolate SMAG_U14152
            k141_3778878, whole genome shotgun sequence.
ACCESSION   DASRZF010000445 DASRZF010000000
VERSION     DASRZF010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970303
            Sequence Read Archive: SRR8554511
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiales bacterium (soil metagenome)
  ORGANISM  Polyangiales bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:13:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,910
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,860
            CDSs (with protein)               :: 3,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Polyangiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3778878"
                     /isolate="SMAG_U14152"
                     /isolation_source="soil"
                     /db_xref="taxon:3034171"
                     /environmental_sample
                     /geo_loc_name="Finland"
                     /lat_lon="63.0472 N 21.7116 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VFG22_19530"
     CDS             complement(<1..190)
                     /locus_tag="VFG22_19530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6418498.1"
                     /translation="MFYSVLIVSEAIARARSAKASMYRSLVTASAEHLFATQGYEKTK
                     IQDIAAASGLSLGTLYSVF"
     gene            255..446
                     /locus_tag="VFG22_19535"
     CDS             255..446
                     /locus_tag="VFG22_19535"
                     /inference="COORDINATES: protein motif:HMM:NF018595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HET6418499.1"
                     /translation="MRIKVDFDLCQGHANCMGEAPEVFQVDEQGFLNILQEEPPEELR
                     EKVELAVKYCPTGAIAMED"
     gene            complement(462..1265)
                     /locus_tag="VFG22_19540"
     CDS             complement(462..1265)
                     /locus_tag="VFG22_19540"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HET6418500.1"
                     /translation="MVLIKRFFRRVVLDSWKEADAEADTAGAERLDFRPIVILVTTAL
                     VLTLARYFGGNSTFSQIFPYDSKVQTRAEWDLLARAWWSGFRFLTYVVIPMLTIILMP
                     GERIRDYYVSFRGFFRHLWIYAGLYLLVLPFIVLVARQDQFLQTYPFYKYANQSVSHF
                     LKWQVLYAAQFLSLEFFFRGYMLQGLRHKFGYGAIFVMVVPYCMIHYPKPLPETLGAI
                     IAGVALGTLSMRTRSIWGGVFIHVGVALTMDLLAVSECPPPGGPPCRSR"
     gene            1324..2298
                     /gene="gspC"
                     /locus_tag="VFG22_19545"
     CDS             1324..2298
                     /gene="gspC"
                     /locus_tag="VFG22_19545"
                     /inference="COORDINATES: protein motif:HMM:NF041515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HET6418501.1"
                     /translation="MAAASNKRVRNLIVLATLALSAFFSAQGATALLAAKVLSVAPEE
                     SRSPSKPTPSARAMRKHDPMLILRRNVFNSSLGDLSETPIAMPELPLEDLPPEDVETP
                     CKGSLRLIGTVVIPGELERSLAAIVGSDQKAGLHRGGAEVEGSKIRAIHSDGVVLQSS
                     AGLCRLAMFEVDGAASAPLQQKPALGRAQSRRNAAGPQSDRNAGLSDEEMEEGIERIN
                     DSNFNLSRSLLNKVLDNAGKLIGIAAVRPKIENGQSVGLEMGGVRPDTLLTKIGLQNG
                     DVLESVNGQSLSSPDAALGAYTTLRTADKFNLSIRRGGQPMTINYNLQ"
     gene            complement(2315..>2540)
                     /locus_tag="VFG22_19550"
     CDS             complement(2315..>2540)
                     /locus_tag="VFG22_19550"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6418502.1"
                     /translation="RPQVMFTSFGDSSLSFELRVYIRTVAQLFPTRHELHMAVDKAFR
                     EAGIEIAFPQRDLHLRTVPENWKPPSNRST"
BASE COUNT          592 a          698 c          794 g          456 t
ORIGIN      
        1 cgaaaaccga atacagagtg cctagcgaca ggccagacgc ggccgcgatg tcttggatct
       61 tcgtcttctc gtagccctgc gtcgcgaaga ggtgctcggc cgaggcagtg acgagagagc
      121 ggtacatcga cgctttcgcg ctgcgggctc gagcaatggc ttccgacacg atgaggacag
      181 aatagaacat cattactttt ttcgtaacaa tattctatat tccgatatat acgcgacata
      241 cggagggcat tttcatgcgg atcaaggttg atttcgatct ctgtcagggg cacgccaact
      301 gcatgggcga ggcgcccgag gtctttcagg tcgacgagca ggggttcctg aacatcctcc
      361 aggaggaacc gcccgaagag ctccgggaga aagtcgagct agcggttaag tactgcccca
      421 ccggtgcgat cgcgatggaa gactgaccgg gcaccgagcg actagcggga gcggcatggg
      481 ggcccgccgg gtggtgggca ttcggatacg gcgagcaggt ccatcgtgag cgcaacgcct
      541 acgtgaatga atacgcctcc ccagatggag cgcgtccgca tcgaaagcgt cccgagggcg
      601 acccccgcaa tgatcgcgcc cagggtctct gggagcggct tcgggtaatg gatcatgcag
      661 tacggcacta ccatcacgaa gatggcgccg tagccgaact tgtgacgaag accttgaagc
      721 atgtaaccgc gaaagaagaa ctcaagagac aggaactgag cagcgtagag gacctgccac
      781 ttgaggaagt gactcaccga ctgattggcg tacttgtaga aggggtaggt ttgaagaaat
      841 tgatcttgcc tcgcgacgag cacaatgaat ggaagcacga gtaggtagag acctgcgtaa
      901 atccagagat gtcgaaagaa cccgcggaac gaaacatagt agtcccgaat acgttcaccc
      961 ggcatgagaa tgatcgtgag catggggatg acgacgtagg tgaggaaccg aaaacccgac
     1021 caccatgcac gggcaagcaa gtcccattcc gcgcgcgttt ggaccttcga gtcgtaagga
     1081 aagatctggg aaaatgtgct gttgccgccg aaatatcgcg caagggtgag gaccagcgcc
     1141 gtcgtgacga ggatgacaat cggccggaag tcgaggcgct cggcacctgc agtgtccgct
     1201 tccgcgtcgg cctctttcca agaatccagc acgacgcgcc ggaagaaccg tttgatcaaa
     1261 accatcgaag cgcgggacta taaccgattg acggcggcga ttgtaggcta agctctaacg
     1321 taaatggcag cggcctcgaa caaacgagtg agaaacctca tcgtgctcgc tacgctggca
     1381 ctcagtgctt tcttctccgc tcagggggcg acggcccttc ttgcagccaa agtgctcagt
     1441 gtcgcaccgg aagagagccg ttcaccatcg aagccaactc catccgcgcg cgcgatgcgc
     1501 aaacacgacc ccatgctgat cctgaggcga aacgtcttca attcatcgct cggcgatctc
     1561 tcggagacgc caatcgcgat gccggagctg cctttggagg atttgcctcc ggaggacgtc
     1621 gaaactccgt gcaaagggtc gcttcgactc atcggcacgg tggtgattcc aggagagctc
     1681 gagcgctcac tagctgcgat cgtaggaagc gaccaaaagg caggccttca ccggggcgga
     1741 gcggaagtcg aaggatcgaa gattcgcgcg atccattcgg acggggtcgt gcttcagagc
     1801 tctgccggtc tctgtcgtct cgcgatgttc gaggtcgacg gggctgcgag cgcgccactg
     1861 caacagaagc ctgctcttgg aagggcccag agccggcgca acgcggccgg cccgcaatcg
     1921 gatcggaacg ctgggctgag cgacgaggag atggaggaag gcatcgagag gatcaacgac
     1981 agcaacttca atctctctcg atctttgctc aacaaggtgc tcgacaatgc tgggaagctc
     2041 atcggaatcg cggccgtccg tcccaaaatc gagaacggtc agtccgtggg cttggaaatg
     2101 gggggcgttc ggcccgacac gctgttgacc aagatcgggc tccaaaacgg cgatgtcctc
     2161 gaaagcgtca acggtcagtc tttgtcgagc cccgacgctg ctttgggcgc gtacaccacg
     2221 ctgcgaacgg cagacaagtt caacttgtcg attcggcgag gggggcagcc catgacgatc
     2281 aactacaact tgcagtgaga ccgcatcgca tgactcaggt tgagcggttt gaaggcggtt
     2341 tccaattctc cggcacggtc cgcaagtgca agtcgcgttg cgggaacgcg atctcgatgc
     2401 cggcctcgcg gaaggccttg tcgacggcca tgtgcagctc gtgccgtgtg gggaacaatt
     2461 gtgcgacggt ccgaatgtag acgcgaagct cgaaagaaag cgagctgtca ccgaagctcg
     2521 tgaacatgac ttgcggccgc
//
